LOCUS       DAOSIF010000001        36167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_1344_length_36167_cov_5.194561, whole genome shotgun sequence.
ACCESSION   DAOSIF010000001 DAOSIF010000000
VERSION     DAOSIF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 36167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36167
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1344_length_36167_cov_5.194561"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="PLT35_00005"
     CDS             complement(<1..2022)
                     /locus_tag="PLT35_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG27632.1"
                     /translation="MSTRTVVSAGTRLLLVVLAAAAAAPATHAQKAAPAQRPDEAYTR
                     LIKEYTQDPRISTELVDHMPASDKVPSPLKFFGRIPGTPGELTYAKDIHRYYEALAKA
                     SPRCRFWTIGKSEEGRDMVLLAVADEATIRNLDAYKAHLNALTDPRKTTEARARQLIK
                     TAKPVYWITAGLHSGETGGPETLIELAYRLAVEETPFIQQIRNNVIVLVTPVLEVDGK
                     EKHVDTYYYGKKTGKPRPPMVYWGKYVAHDNNRDAMGQVLKLTQNVNRAFLEWKPTIL
                     HDLHEASNYLYTSTGTGPYNEALDAITINEWWALANTEVMEMTKRGVPGVWTYGFYDG
                     WTANYLFYVAHTKNAVGRFYEVQSYGPDIVENLRLGTNATSREWYRPNPPLPSIKWGP
                     RNNVNIQQSALLIALNYVGKNREVFLENYWLKNQRAVTRGRSEAPFAWVIPVAQRRKA
                     EAADLVNNLRRQGLEISRADAPFKAGALDVRAGDYIVRADQPYRTMAEMYFSVQNFAP
                     GNPRPYDDTGWTMPLMRNVELVPVAEKEVLERPMTLVTADVLAPGGIEGSGPVLVVDH
                     TSDNAIVTFRFRHAGVKMLAAEEDFTLAGHRFRAGAMIVPGADRAALEPTLKELGLSA
                     WAVPAAPEVKTHDLDVPRIGYVHAWSSTQNEGWVRAAFDHYGVPYTYFGD"
     gene            complement(2198..4297)
                     /locus_tag="PLT35_00010"
     CDS             complement(2198..4297)
                     /locus_tag="PLT35_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG27633.1"
                     /translation="MRAALAAGRAACASLALGAAAAVAAGLAADARLAARARQGPVFK
                     SGVEVIAIDVRVIDKDGRPIGGLRPEQFEVSIDGRPRRVVSADFVEYAAGDGVRAAVG
                     KPEDVRRPRFSSSEEAAAAPSPGRLIYLAVDQGSFKPLAARGAMEAARRFIDRLQPGD
                     RVGFVAFPAPGPLVEASRDHAAARAATAQIVGRALPLKSDGLDKNVSLGEAIDIRAGD
                     AIVLEKVLARECAGLGPSARPACELAVRDTALAVGHSAEMQATRSLAGMESIVRSLAR
                     ISGRKTLVFLSAGLPVSDRSGRDLQFLPWMTSLGREAAAANLNLYVLHVDSGFLDAFS
                     AENRTVSDALDRDLSLTSGGLEAIAGSGGGTLIRVVAGADHAFDRVQRETAAEYLLGV
                     EPAQSDRDGRPHRIAVKVAVDGAEVRSRRELVLAAASRVPGKPIDPVRSPAPVRPVAA
                     AFAGRPSLDLPVSVATWVMTARSGRHTVYVSADIGGDFAGPAGVEVMYAFTDAAGRTH
                     APIERSDILLPRRTGRPGSLAFAVRDELEPGSYTLRFAASDASGRSGWTDHRFTVAPR
                     TAGTVEFSDLLLLEPQQDGEAMPAIVTTGALRLRAVEAHLEVAPLAPDAAVAEVTIGV
                     ASATDPALLVSESAFLSRGADRAAAWTAVAYLDLGELPPGDYVAMAIVSNGDRELGRV
                     DRPFRIEGVPPQGLPFP"
     gene            complement(4300..4779)
                     /locus_tag="PLT35_00015"
     CDS             complement(4300..4779)
                     /locus_tag="PLT35_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG27634.1"
                     /translation="MRLKRYYSSRDVTALTGLSADQLRWWERQGLVQPAIAPRRTETG
                     GYTERRYTPVDLLEIMVLADLRRRGFSLTRLRDLVDKLSSHFGVRLYEATGQSGPVTL
                     LTDGGDVYVRTPDGRFFNLLKNPTQPLLVIGEEAGFKVLASRARPRKRRPKKPAAGA"
     gene            complement(4793..5524)
                     /locus_tag="PLT35_00020"
     CDS             complement(4793..5524)
                     /locus_tag="PLT35_00020"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOG27635.1"
                     /translation="MRLLDRLVQDGDRLFRWRSYLPLILVPVVIAGVLATPPPFSARR
                     TERLWELGSTLLALFGLAIRAWAVGSAPEGTSERSTVNPRASRLRTSGPYSLVRHPLY
                     LANGLMALGVVLFPGVWYLPLVLVPAFLLYYERIAVREEAFLLERFGREFEEWADRVP
                     AAVPSRAAYVRATEPFSWKKVLRHEFHGLFVVAASVFAIDAMQESWRARAWRADGPWA
                     WFAGASAVLFVVFTALKRGTRLFDH"
     gene            5732..6751
                     /locus_tag="PLT35_00025"
     CDS             5732..6751
                     /locus_tag="PLT35_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27636.1"
                     /translation="MNKLATIALAGALVAAGAAAGAYLALREHPAGTAGERLQARAGA
                     ANEAGAGAAGPAPEYRQAHVETVRAATAQLTAAGGLTGDAGTRGAAAQASGPSRPSPV
                     QAGSSVTAEPSGEAGVSAADTPGSEAPAALVWAAPDPRQTAAPDTPAEAPVGTPEEKG
                     PAPRTFEEFVVPADSVLGIQLASSVSSEVARVEDPVEARVIRDVLVGTSVAIPAGTRI
                     VGSVIAVERGGKLRTSAKIGVRFHTLVLGDGTRVAIDTASVSREGKSPGKETAAKVGG
                     AAIGGAILGGVVGGRTGALIGGAAGAAGGTAAQAAGGRNPAVLASGTVLTIRLREPAT
                     VVVER"
     gene            6839..8290
                     /locus_tag="PLT35_00030"
     CDS             6839..8290
                     /locus_tag="PLT35_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOG27637.1"
                     /translation="MSTAASLGDMSADEFRAHGHAIVEWIARYMEHPERYPVLARVAP
                     GDIRRALPAEAPDSPEPFDGILADFDRILMPGMTHWNHPGFMAYFATSASGPGVLAEF
                     LSAALNQQAMLWRTSPSATELEAVALGWLRRLLGLPDSFAGVIYDTASMSSLHALAAA
                     REAAVPHVRRLGLAGRADVPRLRVYCSDQAHSSIDKGVILAGFGHEALRRIPCDAEFR
                     LPPDALGAAIAEDRSAGVLPVAVVATVGTTSTTSVDPVAEVAGICEAEGLWLHVDAAY
                     AGAAAMLPEHAHILAGAERADSLVVNPHKWLFTPFDLSAFFCRRMDVLRQAFSLVPEY
                     LRTPEAAANLMDTGVQLGRRFRALKLWMLLRYFGADGLRGRIRHHVGLGRQLARWVDA
                     HPDFERLAPAPFSVVCFRARPRGREWSEAGLARLNEAILARVNASGEVFISHTVLHGR
                     FALRAAIGNLQTTERHVARAWELVQEALDAELA"
     gene            complement(8336..9133)
                     /locus_tag="PLT35_00035"
     CDS             complement(8336..9133)
                     /locus_tag="PLT35_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOG27638.1"
                     /translation="MAGPDPGWIPVNAPPLAQGRARPLVATFLERPHRFAAICRRRDG
                     EVVAAHVPNPGRLTGVLAPRCTVLLDGPFPPPRSLLYTMLAARPADGWIGTNTLYANR
                     VFPALVSHGLFPELGSRLGAVRAEVAHGRSRFDFQLGKTLVEVKSVTLAATRSAARAG
                     RRSAAAGSDRGAATARVGAFPDAVSARAARHCDELAALARARRRAAIVFVAQRGDVAS
                     VAPADDIDPDFGAAIRAASAAGVLVLACALDMTPAGATRARRIPVHL"
     gene            complement(9133..10482)
                     /locus_tag="PLT35_00040"
     CDS             complement(9133..10482)
                     /locus_tag="PLT35_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27639.1"
                     /translation="MNCRTAVRTAASAALAMGAILAGQQPPPVPVVLVTGARILDAAR
                     GRYLAPAAILVEGDRIAAVTPGPPSPMPAVTAHVKADGAVVVPGLLDAHAWGAPAPDL
                     DADYFYLMGLAHGVTGYRVLNARTSWAVAQRARAAAGDVLAPRLWTSGRGINQGASPD
                     RWLFDAPDARAAAAEAARQVAAKVDWIAGYEALGPESYQALAAAARQAGVRVSGRPGA
                     ASMADLAAAGVASIETLAFPLKASAGPTDDPWPEVGARDLAALQAGLLRRRVTLVPLM
                     AAAAARAFPEEALKDPSMALLPAARRAALEARLSRTAADAPKRRRAWASQAAFLVRFV
                     RAGGRVAAGTGFEFDGYPVPGGGVHRELAAFVRAGLTPAEAIRAATAAGSALVGAQTG
                     GAGLSAGAPADFFVVTGDPLTRIEDLSAITHVVRAGELLDPKALLALARRGAAPKAK"
     gene            10638..11654
                     /locus_tag="PLT35_00045"
     CDS             10638..11654
                     /locus_tag="PLT35_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOG27640.1"
                     /translation="MREIVVTRHGSPEVMEIRERPIPEPGPGDVRIRVKAAGVNFADV
                     LARMGLYGDAPPLPFVPGYEVSGYIDALGPGPSRHERGRHVVALTPFGGYAEYAVAAS
                     DFVWNVPPPLSHNEAAAMPVTFLTGSVALYRMANLRSGESVLVRSAGGGVGVAAVQLA
                     RLRRATVIGTASSAKHSALRSLGIDHVIDDRRADLEAEIARITNGRGVDVVLDPLGGA
                     SLEQSYRLLAPLGRLISYGTQSCVDGFERDDARVQRVLDSRPRFDPLDLMRANKGVFG
                     LNLGHLWKERRYAAWAMEALLTDFAGKRLRAVVSKTFPLAAAASAHACLQDRANVGKV
                     VLTI"
     gene            complement(11689..13263)
                     /locus_tag="PLT35_00050"
     CDS             complement(11689..13263)
                     /locus_tag="PLT35_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOG27641.1"
                     /translation="MMIAIAAVGATAQDGPGPPELRAVWVDAFHHGIRSPAEAAQLVA
                     DAQRTHVNTLFVQVRRRADALYTGGLEPPLDDPSYDPSFDALAHIVSAAHAAGLQVHA
                     WVNAMPVWRDEAPPKDARHLFNQHGPAAAGERCWLTRMRDGTRRFPVGYFLDPGHPAA
                     CDHLVAVCLDIVRRYDVDGLHFDYIRYPETEGAPLPRGADVGYNQVSVARFQRAYKRA
                     RAPLPDDEAWTRWRREQITSLVRRISIEARAIKPRLRVSAAVVAWGPPPASARDFENA
                     SPMQRVFQDWRGWLEEGLIDLAVPMNYAREHDERGREWFDGWIAWEAKHKGDRQLAVG
                     VGGYLSAPEGVLAQISRVRSAAREARADGVSLFSYYQPSMEPVMPEQPAAAQGAASAS
                     QAQPAGAPPPPAPDRLDYLVRGAAGAPAAFTGPAPVPAMAWIDAPAHGALAGSIAGGA
                     GALADRRTVRIRQAGPPHETRTITSDASGWFGAARLPPGTYQVQLEDARGKPIGPRKL
                     VRVAAGAVARVTLAAR"
     gene            13423..16044
                     /locus_tag="PLT35_00055"
     CDS             13423..16044
                     /locus_tag="PLT35_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG27642.1"
                     /translation="MKPAASVAIVAMLTVGAPLPAAGLAAPQAAAQASAPAAALTPAP
                     VAPTVDQLVSLKRAGSPAISPDGTMVAYTVREANWEEDAYETEIWIADVASGAVRQLT
                     NGRKSSDAPAWAPDGSRLAFASTRSDKRQIWLIRPSFGEAEQLTHEREGVERFAWSPD
                     GRQIAFTQLDPKSEAWRERDKKYGEFEIVGADHRMSHLWVIDVESKRTRRLTKGDFTV
                     GSFDWSPDGTRIAFDHTVNADPASSGTSDISIVDVAGGAITPLVRQEGPDTRPLWSPD
                     GSHVAFASAMARPFYYYTNSRIAVIPAAGGAITNATAGFDENASPLMWAAKGFLLFSA
                     AQRTGAHVFLLHPSSGELRQVTAGAQQAYSGFSATRDGSAMAFIRSGPGEFPEVCVLT
                     LSGSDSSLKKLTSLGAQAASWPQPSQEVIAWKSLDGTLIEGVLHKPADFKAGTRHPLL
                     VVIHGGPTGVSRPVPYGSTSAYPIDIWLSRGAIVLEPNYRGSAGYGEAFRSLNVRNLG
                     IGDAWDVLSGIDHLVAQGLADTDRVGVMGWSQGGYISAFLATHDSARFKAVSVGAGIS
                     DWMTYYVNTDIYPFTRQYLKATPWDDPKIYADTSPITYIKRASAPTLIQHGELDRRVP
                     IPNAYELLRGLLDHGVPAKLIVYKGFGHGLTKPKAHRAAMEHNLEWFGRYIFGEAPPA
                     QARQAARVEPFLGVLNFARIDDRFATGGAMKSEAAAELKRQGFKAVLNLRAASEPGAD
                     VEAAKAAVEAAGMKYIHIPFVRTDPAADAAVDAFLAATKDRSNQPLFFHCGGGNRAAA
                     IWAVRRVMVDGWPRSRAVAEADSVGVISGAMRKWAEEYLSAHGRQEPEGARSRNPESW
                     ILQNSMRTPATSVRPHW"
     gene            complement(15996..18335)
                     /locus_tag="PLT35_00060"
     CDS             complement(15996..18335)
                     /locus_tag="PLT35_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG27643.1"
                     /translation="MPTALSMLVVLVAAQAPAAQTPAAPAPSPRFVPACHVGGTVVDE
                     RTRAGVSRAVVVLRAFSPERLQDAASPPSCRGGCQTVADGDGRFAFADLLPGDYALTA
                     SIEGFAESAPLEVRLDGQPCAVTADIPFRFQMLAEARAGLPRALGATPIPAAIAPVLT
                     GAAIAETPGALEDLSRAFQSLPGVAGSQDNRNDLLVRGGGALENQMRIDGFDLPNVNH
                     FGAQGGSGGALSILPPWLIERGSLEVGGFSVAFGDRMSSVADVSIRPGRRDRVHAAVG
                     AGIGGLTAIGEGALGARGSWLVSARRSLLDAVFSKLTDEIVPAYADVLVKAEHRAGEK
                     HRLTVLGLGSRDEASGHDEKAGDDELSGSETTALAGVRFDSSWSARAFSSVVASLSSI
                     DVDARAVDGAVVDAIDAGKETEVRIRADVRRKRTFVGDILVGGAVKTYRYDYELFVND
                     LWTPYNALNRDLSSRQRRSFTDLAAYAEAERAIAGRGRLLAGVRIDRWGAASLTAGSP
                     RVKAEIVAGRSVRLVGHWGIYRQGVPYIWMASAPDNIGLAPMASRQFGGGLDFEPRPW
                     LRLAVEAFGKRYRNYPVDPAVPSRVLVSSAADFEPPYVGPLVGAGRVRASGIDSLAQL
                     SPLPGLEVATNYSYWKVSQSGLDRAWRPAEHELRHQARLEVRYRPDGPWSAGFRLRHA
                     SGRPYTPFSVPLSVKRGRAAYDLTQINALNYPYYRRLDVRVDRSVRTRFASAAAYLEV
                     DNLLNRRNVLTYRWNRALKGPKPVHQWGRTLVAGVRIEF"
     gene            complement(18548..19669)
                     /locus_tag="PLT35_00065"
     CDS             complement(18548..19669)
                     /locus_tag="PLT35_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG27644.1"
                     /translation="MRRLLFLVRKELQELRRNPRLFPLIFLAPVLQLFILGYAASTDV
                     RNVPITIVDGDRSQASRELIGRFEGSPYFSVSRVTSDEGDVLRDIETRRAWMALSIPP
                     EYGESVAAGRPVTLQVVADGTDSNSAGLGLTYATTLIADKASELALARRPRGRPAAGA
                     IEARIRVWFNPQLLSRHFMVPGVLALLLMVITSVLGSMAVVREKELGTLEQLNVTPLR
                     RWELIAGKLLPYGLIGTLDVFLVVGVAALWFEVPLRGSFPLLLGLSMVYLLCTLGLGL
                     LVSTLARNQQQAMMTAVFFFMMPMIYLSGFVFPIENMPAAIQPLTYLVPLRYYLVIVR
                     GIFLKGVGLEAFWPDALLMLAWGAAVLALATARSRKTLA"
     gene            complement(19669..20781)
                     /locus_tag="PLT35_00070"
     CDS             complement(19669..20781)
                     /locus_tag="PLT35_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG27645.1"
                     /translation="MRKALAVGRKELAQVLRDRLTLAILLLVPVLLLLLFGYALNWDV
                     RHVRLAVMDRDRTAESRQLLSSFVNSTYFDVVATPSSMAGIERLMDRGEARAALVIPE
                     GMTRDLAAGRTAAVQVLVDGDNANTASTVFGYAQAIVRGASADRLGASTGAPAAPLSV
                     EPRIWYNPELASSLFLVPGLIAFIVMISCVVATALSIVREKERGTWEQVRMAPIATPS
                     YIIGKSVPYFLLSLAAAFLVIAAAMLLFGLPMRGSWPLLAGSVALFVLGGLGTGLLVS
                     TLFDTQATAFLASLLISLLTTLVLSGFIFPIASMPAVIQYVTFIVPARYFIVILRGIL
                     LKGIGFAPLALEFAALGVYVTAVMSLASIRLARERG"
     gene            complement(20778..21731)
                     /locus_tag="PLT35_00075"
     CDS             complement(20778..21731)
                     /locus_tag="PLT35_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG27646.1"
                     /translation="MPSSLSAPAVTVRDLSRRFGRFLAVDRVSFDVGRGEIFGFLGSN
                     GAGKSTTIRMLCGLLRPSSGSAVVDGIEVTRDPEEVKQRIGYMSQRFSLYELLTVQEN
                     IRFFGGMYGLDDRALRTRGAYVLKMAGLEGLERTLARDLAGGWRQRLALGCAILHEPR
                     ILFLDEPTGGVDPVSRREFWRLIDGLSGQGVTVFVTTHYLDEAEHCHRLALMHAGRLA
                     ALGTVAELKRVFSGRLVVEVRTTSPMAALRALDGVPSVEKSSPFGTALHVWLRPGETD
                     VAPLRDRLRASGIEAESLDLVEPSLEDVFMDVVEREERTAA"
     gene            complement(21739..23157)
                     /locus_tag="PLT35_00080"
     CDS             complement(21739..23157)
                     /locus_tag="PLT35_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG27647.1"
                     /translation="MNDHARRPTAAAFLIAALAATLGAAGPGAAASTAVPPPPVPPPA
                     APAPATPPAPSGAVTLSLDDAVARALQASHRIGEALARQEAADAAVAARKAANMPTIS
                     LAAGYLRTNHVNEFGIPAMGAAPARIIYPDVPDNWRSRLDVQWPIYTFGRVEAAERAA
                     RAEAAASGRDTATTRADVRLDAARAFWAYATAIESVRVVEEALALVDAHLRDVRNMRA
                     VGLLAPNDVLSAEARRSRQEVQLIEARTIRDVAEADLRRVTGIPPDVSIAVDAALEAA
                     PAPIPSGEALLAEAQAKRPERQALESRAAAVGERQAAAAAGRRPSVALTAGVDYARPN
                     ARIFPRAAVWNDSWDLGVSVAWPLWDGGRAKAEIAEAAASRRALAERLAEFDAALGFE
                     VRQRRLDLESAHASIRAATDEVASAAEARRVVAERFKAGLVTSTEVLDAQQELLAAQF
                     DRTRALAAARLAAARLDRAVGR"
     gene            complement(23154..24092)
                     /locus_tag="PLT35_00085"
     CDS             complement(23154..24092)
                     /locus_tag="PLT35_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG27648.1"
                     /translation="MALALELERVTKRFGPTDAVRDVSYAVEPGEMFGLIGPDGAGKT
                     TTIRLLCGLLRASSGSIRVLGQDPVRAHGAITRKVGYFSQKFSLYGDLSIDENIAFFA
                     EIHGVADYRERRNRLLDMMQLTPFRGRLAERLSGGMKQKLALACTLVHEPEVILLDEP
                     TTGVDPVSRREFWHLLSEFLSQGITIFMATPYLDEAERCSRVALMSQGRVLALDDPGS
                     LRAAFPDDLVEMLAPRQDEAARIALLDPAVRDVQRFGERLHVRVPRGEGPGVTRRLGA
                     ACREAGIDVTGLRTVPASLEDVFIDRVVTTPDQGAA"
     gene            complement(24214..25278)
                     /locus_tag="PLT35_00090"
     CDS             complement(24214..25278)
                     /locus_tag="PLT35_00090"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG27649.1"
                     /translation="MSYREPRAARLARAAAVAAAAATLAASLACRDEPAADHVRVSGY
                     VEATEIRIAPEVGGRLLELRADEGDRVKAGDTVAQLDTADVTLALRRAAAERQAADAQ
                     LRLLQAGARAEDIRQAEAQHAAAEADLAAARQELASAERDLQRFDTLLAANSGAEKPR
                     DDAQTRRDVTRDRVRAAEARARAAAETVARLRAGARREEIDAARARLAAADAQIATLD
                     KALNDATVLASAAGVVTQKLVDAGELIAPRTPILVVADLDRVWANVYVDEPLVPRVTL
                     GQSARVFTDAGGPGIDGTVTFISPKAEFTPRNVQTAEERSKLVFRVKITVDNRSGVLK
                     QGMPVEAEIPLKEQAPGAGR"
     gene            complement(25275..25976)
                     /locus_tag="PLT35_00095"
     CDS             complement(25275..25976)
                     /locus_tag="PLT35_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG27650.1"
                     /translation="MSVKTPRRRTPGPRRSPATPLPPSDSRQRILHAAAAEFALRGFE
                     PATVDRIARRARLNKAMIYYHFRSKRALYTSVLRTLFTLMGDCLAATAASTDPPQVRL
                     DRFVSAFVLEGQRLAHFAPIMLRELASGGRRLDEETLAVTARVAGILRSILDQGRRDG
                     AFAPVDPLLLALTTIWPIVAYLASGPIRHAFARISHAEPGRLDPDRFILHLQTLNRRA
                     LTPAAAADIPTGVTS"
     gene            26171..27145
                     /locus_tag="PLT35_00100"
     CDS             26171..27145
                     /locus_tag="PLT35_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOG27651.1"
                     /translation="MRVVIAGGSGFLGRALAASLARDGHRIQILTRRRLRQAPPLPQP
                     PADASSPAAPAPVEYITWQADGSVGPWAGPCRGADTIVNLAGESIGSRRWSLARKDAL
                     VQSRLLATRSLVRFIRQSSPPPSAFLSASAIGLYGDRGSDILAEDAEPGSGFLASLAA
                     AWEREARDADGGPTRVVLLRTGIVLDPRSGALARMLLPYRVCAGGPLGPGRQFMSWIH
                     RDDWVSLARWAIRTPEVRGPLNLTAPHAVTNATFARALRRVLRRPSLVRVPAFALKLL
                     LGEMAGPLLFYSQRVVPRKALDGGFTFAHPEVEGALAHLLDSKAAPAA"
     gene            complement(27177..28421)
                     /locus_tag="PLT35_00105"
     CDS             complement(27177..28421)
                     /locus_tag="PLT35_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOG27652.1"
                     /translation="MTRRDFLRHGAGAFTVGLAGPAVLADLARAQGSGRRALVVCFFA
                     GGNDALSTLVPYTDPYYYSRRPSIAVAAGDALQVGSDSSGVSLGLHPGLPALRAIFNE
                     GRLAIVQRTGYANSTRSHFLGTDVWGSANPASPQSHGWLGRYLDTLPQPVDPLAAWDA
                     VREIPRALTSDHVPVPAIPSASGYVFSAVNGGAEAARERVFAERLASRDASDRPHLAF
                     VNAGIDAAMNTLDKVALVAKYAPAAVYPSTPTGTTLKTVAGALVKGVGTRVFWVQLGG
                     FDTHAAQAATYPRLMTEFNDAVVAFYADLRAQGLWGDVLLMTFSEFGRRAYENGSAGT
                     DHGAAGLMFAAGGAVRGGLYGTAASLNPAPDNPALENSGGDVGYETDFRSVYARVIDH
                     WLGGDSVSILQGDFRRPGLAFV"
     gene            complement(28473..29930)
                     /locus_tag="PLT35_00110"
     CDS             complement(28473..29930)
                     /locus_tag="PLT35_00110"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOG27653.1"
                     /translation="MVYRDEVLRHLLRRAGFGASDAELEYYSRLGRRGTVETLVDFEQ
                     IHDDVDAFVGAPGYVGVKAAGAVFSPNTVIGDARQRWLFRMIHTARPLQEKMTLFWHN
                     HFATAYSKIAGAYGAADATRLMAARAAEDPAGQEGQVETIRRLCLGNFRDLMVAMAKD
                     PAMLIWLDGRYNTKARPQENFGRELLELFSMGVGFYTEPDVYAAARVFTGWNLARTAA
                     NPSYPRYEFAYNASLHDTAAKTFSFAIYPDGGRTIPARSADGGLQDGLDLIAAVVAHP
                     QTARRLVSRFWSFFVSEVNPPTERFVSGLARDVLARGFDLRAVMRRLLQSPEFRSEEN
                     YHTKYSWPAEFVARSLKEVGWEGFTLNNAVSQMASMGQVLYEPPDVAGWEQGPGWISS
                     GGMLARMNFAAQLSRAQAAALVKAARPHASSPGAVLSHEIGRLSPAPFGREAYDDLLA
                     YLTASGAWTGSDAQLAMKVPGLVRLIVGSGEYVFV"
     gene            complement(30048..32753)
                     /locus_tag="PLT35_00115"
     CDS             complement(30048..32753)
                     /locus_tag="PLT35_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOG27654.1"
                     /translation="MKRSRIVLAAALLAAFAAGGALPAQMPPIADAQGQVGLGLLLRK
                     LRTTAVFMEATAHPDDEPNGVLAALSWGQGVRTIVATATRGDGGQNEIGPELFDALAV
                     ARTEELVSVHALDGAEQYFTRAVDFGYSFSVDETLDLWGRQEILGDFVRLIRMTRPDV
                     IAAMRPDGEGGGQHHQASARIAREAYLAAGDPARFPEQLREGLRAWQPKKFYFTGQYG
                     FAGEPAPAAGAKLTTVDTAAFDPLLGQSFADIGSRARSYHKTQGMAQLFWLPGPAAVR
                     FTLVESAIPGLKGKDEASLFDGLDTSLAGLARFVPGEAPAGLTSALASVAGRISDAAA
                     QAASGGPAAAVAPLAAGLTSLRALRAQLASRAFGLPEDASAEIDARLALKEEQFTDAL
                     LAASGVRLDALADDGLVVPGQPVTVTLIAANGGREAVVLTQTGFRGLDGEAGVCRTGS
                     IAPAGVYRCDAPLAVSGQARLSGQHWRRLPTGARYQFDADAPFGVPFRPTPFRVRFEL
                     QVGGAALSVERPVQYRYEGNIFSGEKRMDLLVVPRFSVTVSPEVAIVPAGGAPAQAGA
                     ARRAPAASREVRVTVINGSRGPAEAAVSLEIPDGWAVKPASARVSFSREDEAAPVRFT
                     VTPARDAKPGPYTVRAAASAGAERFTTGYQVIEYPHTRRRHLLKEAAAAVKIVEVAVA
                     PRLRVGYIMGVGDQVPAAIEQLGARVTLLDADALAFGDLSAFDVIVSGVRAYERRPDL
                     RANNHRLLRYAEDGGTVLVQYNKFEFNEAQYGPFPAKVSANRVTDERAPVRILEPGHP
                     VFGWPNRIGEAAWRDWVQERGLYFLGEKDGRYLDLVESEDPFPYNKGPKRGALVEAKV
                     GKGRWIYIGLGLWRQLPAGTEGAYQLLANLLSAGHAPVPAGRPAR"
     gene            complement(32917..34146)
                     /locus_tag="PLT35_00120"
     CDS             complement(32917..34146)
                     /locus_tag="PLT35_00120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG27655.1"
                     /translation="MRTRYGVSYWLDRYPKARQPAYPRHRGDVDVQVAILGGGLTGCS
                     AAYVFAASGVRVALIESNRIGQGGSASCPGLLRLEPAVPFLELKHAYGLRAARHLWQS
                     FRRAGLDLAATIRRLRIRCELVPDAALRAGLSEGDGARLRRELAAMRGAGLEGGWLTP
                     ARLRQETGLSGEGAIRSAGDAFIDPYRATLGLAAEAAKRRALVFERTTVTRVRAGRKG
                     VEVATDGGIVRAAAIIVASNGPAAPFKPLRRHFSPMHAFCVLTPPAPSFVRREFGRSR
                     AVVIDQDDPPHVLRRTSDERVLFVGADQPALPARTLEKALVRRAGQLMYELSRLYPAI
                     AGTQPEYAWTVPVWKSADGFPCAGPHRHYPRHLFALGAGHNGVASALLAARVLLRRYA
                     GAPDNGDELFGFTRLLG"
     gene            34276..34995
                     /locus_tag="PLT35_00125"
     CDS             34276..34995
                     /locus_tag="PLT35_00125"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOG27656.1"
                     /translation="MHTRQTPGVVSREIAIHLERHGCPAHAAGAQRYFKDEVKCWGWR
                     TKDLRSYARAVHAALAPDPELLLNVAERLFAEASLEEKGLGVLILQPSMKRFGGREFR
                     RLEGWLDRVATWADHDALTMTLLGPMIVADPRRVARPLRWAASRDRWRKRASAVSLIP
                     GIRQGLFMEEGRIVADRLAADRDDMVQKGLGWLLREWAKHHPAEAIPVLAAIRGRASR
                     LVLRTGCEKLGAADRQRILRK"
     gene            complement(35131..>36167)
                     /locus_tag="PLT35_00130"
     CDS             complement(35131..>36167)
                     /locus_tag="PLT35_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOG27657.1"
                     /translation="VLLSEIAEQPAIIEGLLKAAAPAVERIAADIRGRRLHHVVIAAR
                     GSSDNAARYGQYLFGSHNRLSVTLAAPSLFTRYRTPPAMAGALVVGISQSGESPDLIS
                     VVEEGKRQGCLTLAVTNVAGSPLAAMADHTIVLGAGQERSIAATKTYTAQLAALAMLS
                     TALAENARWSRELAAMPEHVAAALYVERESVVAAAAALKDAGHGVVLGRGFNFSTAFE
                     AALKAKELAYVAVEPYSSADFQHGPIALVEPGFAAMVFNVTGAVSSEVEGLLREIRER
                     GARPVVFSNEPSSLELAAAPVALPAGMPEWVSPIAAIVPAQLLAFHLSRMRGYDPDRP
                     RGLKKVTKTT"
BASE COUNT         5101 a        13056 c        13111 g         4899 t
ORIGIN      
        1 gtccccgaag tacgtgtagg gcacgccgta gtggtcgaag gcggcccgga cccagccctc
       61 gttctgcgtg ctcgaccagg catgcacgta gccgatgcgc ggcacgtcca ggtcgtgggt
      121 cttgacctcg ggcgcggcgg ggacggccca ggccgacaag ccgagttcct tgagggtcgg
      181 ctcgagcgcc gcccggtcgg ctcccggcac gatcatggcc ccggcccgga agcggtgccc
      241 cgccagcgtg aagtcctcct ccgccgcgag catcttcacg cccgcgtgcc tgaaccggaa
      301 cgtcacgatg gcgttgtcgc tcgtgtgatc gaccaccagc acgggccccg acccctcgat
      361 gccgcccggc gccaggacgt ccgccgtcac gagggtcatc ggccgctcga gcacttcctt
      421 ctcggcgacg ggcacgagct cgacgttccg catgagcggc atcgtccagc cggtgtcgtc
      481 gtagggccgg gggttcccgg gcgcgaagtt ctgcacgctg aagtacatct ccgccatcgt
      541 ccggtacggc tggtcggccc ggacgatgta gtcgccggcg cggacgtcga gggcgccggc
      601 cttgaacggc gcgtccgcgc gcgagatctc gaggccctgc cggcgcaggt tgttgaccag
      661 gtcggccgcc tcggccttgc gccgctgggc gaccgggatc acccacgcga agggcgcctc
      721 gctccggccc cgggtcacgg cgcgctggtt cttgagccag tagttctcga ggaacacctc
      781 gcggttcttc cccacgtagt tgagcgcgat gagcagggcc gactgctgga tgttgacgtt
      841 gttgcgcggc ccccacttga tggacgggag aggcgggttc ggccgatacc actcgcggct
      901 cgtcgcgttc gtccccagcc gcaggttctc gacgatgtcc ggcccgtagc tctgcacctc
      961 gtagaagcgg ccgacggcgt tcttcgtgtg ggcgacgtag aacaggtagt tggcggtcca
     1021 gccgtcgtag aagccgtagg tccacacgcc gggcacgccg cgcttggtca tctccatgac
     1081 ctcggtgttc gcgagcgccc accactcgtt gatcgtgatg gcgtcgaggg cctcgttgta
     1141 cggcccggtc ccggtggacg tgtagaggta gttcgacgcc tcgtgcaggt cgtgcaggat
     1201 cgtcggtttc cactcgagga acgcgcggtt gacgttctgg gtgagcttca gcacctggcc
     1261 catggcgtcg cggttgttgt cgtgcgcgac gtacttgccc cagtagacca tcggcggacg
     1321 cggtttcccg gtcttcttgc cgtagtagta cgtgtcgacg tgcttctcct tgccgtccac
     1381 ctcgagcacg ggcgtgacga gcacgatgac gttgttgcgg atctgctgga tgaacggcgt
     1441 ctcctccacg gcgaggcggt acgcgagctc gatcagcgtc tccgggccgc cggtctcgcc
     1501 cgagtgcagg ccggccgtga tccaataaac cggcttggcg gtcttgatga gctgccgcgc
     1561 cctggcctcg gtggtcttgc gcgggtcggt gagcgcgttc aggtgcgcct tgtaggcgtc
     1621 gaggttcctg atcgtcgcct cgtctgcgac cgcgagcagc accatgtcgc ggccttcttc
     1681 cgacttcccg atcgtccaga accggcagcg cggcgacgcc ttcgcgagcg cctcgtagta
     1741 gcggtggatg tccttcgcgt acgtcagctc cccgggcgtc cccggaatgc gcccgaagaa
     1801 cttcagcggc gacgggacct tgtcgctcgc cggcatgtga tcgacgagct cggtgctgat
     1861 gcggggatcc tgcgtgtact ccttgatcag cctggtgtag gcctcgtccg gcctctgcgc
     1921 gggcgccgcc ttctgcgcgt gcgtcgcggg cgcggccgcg gcggcggcga ggacgacgag
     1981 cagaaggcga gtgccggccg aaacaaccgt gcgtgtgctc atggtgccgt gactcctgtc
     2041 gcgcccggcc cgtcggccat gcggcgccgg acgggccgaa cgcaatcgcg aggttatagc
     2101 acgaacggtc gggcgatacc atcggcgcag cggcctgcgg catggtcggg cgcaagaccc
     2161 tggccccgcc cgacgcggtc ggccgcgacc gggcggccta ggggaacggc agtccctgcg
     2221 gaggaacgcc ctcgatacga aagggccggt ccacccggcc gagctcccga tcgccgttcg
     2281 acacgatggc catcgcgacg tagtcacccg gcggaagctc cccgaggtcg aggtaggcga
     2341 cggcggtcca cgccgcggcg cggtccgcgc cgcgggacag gaatgcgctc tcggagacga
     2401 gcagggccgg gtccgtcgcg ctggcaacgc cgatggtgac ctccgccacc gcggcgtccg
     2461 gcgccaacgg cgccacctcg aggtgcgcct ccaccgcgcg caggcgcagc gcgccggtcg
     2521 tgacaatggc gggcatggcc tcgccgtcct gctgcggctc gagcagcagc aggtccgaga
     2581 actcgaccgt gccggccgtc ctcggagcga cggtgaagcg gtgatcggtc cagccgctgc
     2641 ggcccgacgc gtccgaggcc gcgaagcgga gcgtgtacga tcccggctcg agctcgtccc
     2701 gcaccgcgaa ggcgagcgag ccagggcggc ccgtgcggcg cgggagcagg atgtccgagc
     2761 gctcgatggg cgcgtgggtg cggcccgcgg catccgtgaa cgcgtacatc acttcgacgc
     2821 ccgccggccc ggcgaagtcg ccgccgatgt ccgcgctgac gtagaccgtg tgcctgcccg
     2881 agcgggccgt catgacccag gtcgcgactg acacgggcaa gtcgagggac ggccggccgg
     2941 cgaaggccgc ggcgacgggc cggacgggtg ccggagaccg caccggatcg atgggcttcc
     3001 cgggaacccg gctggccgcg gccagcacga gctccctgcg gctgcgcacc tcggccccgt
     3061 cgacggcgac cttgacggcg atgcggtgcg gcctgccgtc ccggtcgctc tgggcgggct
     3121 ccacgcccag cagatactcg gctgccgtct ctcgctggac gcggtcaaac gcgtggtcgg
     3181 cgcccgcgac aacgcgaatc agcgttccgc cgccggaccc cgcgatggct tcaaggcctc
     3241 cgctcgtcag gctgaggtcc cggtcgagcg cgtcgctcac cgtccggttc tcggccgaaa
     3301 aagcgtccag gaagcccgag tcgacgtgca gcacgtagag attgaggttg gccgcggccg
     3361 cctcgcggcc cagtgacgtc atccagggca ggaactgcag gtcgcggccg ctccgatccg
     3421 acacgggaag cccggcgctg aggaagacca gcgtcttgcg cccggagatc cgcgcgaggc
     3481 tgcggacgat tgactccatg ccagccaggc tgcgcgtcgc ctgcatctcg gcgctgtggc
     3541 cgacagccag cgcggtgtcc cgcacggcga gttcgcacgc cggccgggcg gaagggccga
     3601 ggcccgcgca ctcccgggcc aggaccttct ccagcacgat ggcgtccccg gcccggatgt
     3661 cgatggcctc gccaaggctg acgttcttgt ccaggccgtc ggacttcaac ggcagggctc
     3721 ggccgacgat ctgcgcggtc gctgcgcgcg cggctgcgtg gtcgcgcgag gcctcgacga
     3781 gagggccggg cgcgggaaac gccacgaagc cgacgcgatc gccgggctgc aagcggtcga
     3841 tgaagcgcct ggcggcctcc atcgcgccgc gggcggcgag gggcttgaag ctgccctgat
     3901 cgacagcgag gtagatgagc cggcccggcg aaggcgccgc ggcggcctct tcgctcgagc
     3961 tgaagcgcgg ccgcctcacg tcttccggct tgcccacagc cgcccggacg ccgtcgccgg
     4021 ccgcgtactc gacgaagtcg gccgacacga cgcgcctggg cctgccgtcg atgctcacct
     4081 cgaactgctc ggggcgaagg ccgccgattg ggcggccgtc cttgtcgatg acgcggacgt
     4141 cgatggcgat cacttcgacg ccggacttga agaccgggcc ctgccgggcc ctggcggcga
     4201 ggcgcgcgtc ggcagccagg cccgcggcca ccgcggccgc ggcgcccagc gcgagcgagg
     4261 cgcaggcggc gcggcccgct gcgagcgcgg cccgcatgct cacgccccgg cggcgggctt
     4321 cttcggccgc ctcttccgcg ggcgcgcccg cgaggcgagg accttgaagc cggcttcctc
     4381 gccaatgacg aggagcggct gcgtcggatt cttcagcagg ttgaagaagc gcccgtccgg
     4441 ggtccgcacg tagacatcgc cgccatcggt aagcagcgtg acgggcccgc tctggccggt
     4501 ggcttcgtaa aggcgcacgc cgaagtggct gctgagcttg tcgacgaggt cccgcaggcg
     4561 ggtcaacgag aacccgcggc gccgcagatc ggcgagcacc atgatctcga gcaggtccac
     4621 gggagtgtag cggcgctcgg tgtagccgcc ggtctccgtg cggcgcgggg cgatcgccgg
     4681 ctgcaccagc ccctgccgct cccaccaccg gagctggtcg gcgctcaggc cagtgagcgc
     4741 cgtcacgtcc cgcgagctgt agtaccgctt caggcgcatg tgccgccccc gctcagtggt
     4801 cgaagaggcg cgtcccccgc ttcaacgcgg tgaagaccac gaagagcacc gcgctcgcgc
     4861 ccgcgaacca cgcccacggc ccgtctgccc gccaggcgcg cgcgcgccag gactcctgca
     4921 tcgcgtcgat cgcgaacacg ctcgccgcaa ccacgaagag gccgtggaac tcgtgccgca
     4981 gaaccttctt ccacgagaac ggctccgtcg cgcggacgta ggctgcgcgc gacggcacgg
     5041 cggcgggaac ccggtccgcc cactcctcga actcgcgtcc gaaccgctcg agcaggaatg
     5101 cctcctcgcg cacggcgatc cgctcgtagt agaggaggaa tgccggaacg agcacgagcg
     5161 gcaggtacca gacgcccgga aagagaacca cgccgagcgc catgaggccg ttcgccagat
     5221 agagcgggtg gcgcaccagt gagtacggcc cggaggtgcg cagccgggac gcacgcgggt
     5281 tgacggtgct ccgttcggac gtgccctccg gcgccgaccc gacggcccac gcccgaatcg
     5341 ccaggccgaa cagcgccagc agggtgctcc ccagctccca tagacgctcc gtccggcgcg
     5401 ccgagaacgg tggcggcgtc gccagcacac cggcgatgac gaccggcacg agaatcagcg
     5461 gcaggtagct gcgccatcga aacaggcgat cgccgtcctg cacgagccga tcgagcaatc
     5521 tcattgtcgg ccattctgcc gcctgagcag gttgtttgca aatggagaca ccccccgggc
     5581 tcggacgtgg acgattcagc agcggaggcg gggaaccacc cggctgccct tcagctaaac
     5641 tccacggcag gaagccgttt ccgggaaggt cccggcccgc cgttccggca cgggagttgc
     5701 tctctcgtct gtctggaaca gggagacggt gatgaacaag ctggcgacga tcgcgctggc
     5761 gggggcactg gtcgcagccg gggccgccgc gggcgcgtat ctggctctgc gggaacaccc
     5821 ggcaggcacg gccggcgagc gtctccaggc acgggcaggc gccgcgaacg aagccggcgc
     5881 cggcgcggct gggccggcgc cggagtaccg gcaggcgcat gtggaaaccg tccgggccgc
     5941 gacggctcag ctgactgcgg ctggcggcct gaccggcgac gcggggaccc gtggcgcggc
     6001 agcccaggca tcgggcccgt cacgcccctc gccagttcag gcaggctcct ccgtgacggc
     6061 cgagccgtca ggcgaggctg gcgtgtcggc ggcggacacg ccgggttcgg aggccccggc
     6121 ggcactggtg tgggctgcgc cggatcctcg gcagacagcg gcgcctgaca cgccagccga
     6181 ggcgcctgtc gggacgccag aggagaaagg gccggcgccg cggacgttcg aagagttcgt
     6241 ggtgccagcc gactcggtgc tcggcattca gctcgcctcg tcggtgtcga gcgaagtggc
     6301 gagggtcgaa gacccggtcg aggcgcgcgt gattcgtgac gtcctggtgg gcacgagcgt
     6361 cgccattcca gccggcacgc ggatcgtcgg gtcggtgatc gctgtcgagc gcggcggcaa
     6421 actgcggacg agcgcgaaga tcggcgtccg gttccacacg ctggtgctcg gcgacggcac
     6481 gcgcgtggcg atcgacacgg cgtcggtgtc gcgtgagggc aagtccccag gcaaggagac
     6541 ggccgccaag gtcggcggcg ccgccatcgg cggcgcgatt ctcggcggcg tcgtcggtgg
     6601 acgcacgggc gcgctcatcg gcggcgcggc cggggccgcc ggcggcaccg cggcgcaggc
     6661 ggcaggaggc cggaaccccg cggtgctcgc ctcgggcacg gtcctcacga tcaggctgcg
     6721 agagccggcc acggtggtcg tcgagagata ggaggcacgg ggccgggcgc cggcttcgac
     6781 tcagacaggc acaagccgcc cgcctcctcg cgctagaatc tggcgcagga ggcccgggat
     6841 gtccaccgcc gcttcgctcg gcgacatgag cgccgacgag tttcgcgcgc acggccacgc
     6901 catcgtcgag tggatcgcgc gctacatgga gcacccggag cgctaccccg tgctggcccg
     6961 ggtggcgccg ggggacatca ggcgtgccct gcccgcggag gctccggact cgcccgaacc
     7021 attcgacggc atcctcgccg acttcgatcg catcctgatg ccgggcatga cgcactggaa
     7081 tcatccaggc ttcatggcgt acttcgccac gtcggcgagc gggcccggcg tcctggccga
     7141 gttcctctcc gcggccctga accagcaggc gatgctctgg cgcacgtcgc cctcggcaac
     7201 ggagctcgag gccgtcgccc tcggctggct gaggcggctg ctcggcctgc cggactcctt
     7261 cgcgggcgtc atctacgaca cggcctccat gtcgagcctg cacgccctcg ccgccgcgcg
     7321 cgaagccgcg gtgccgcacg tccgccgcct cggtctcgcg ggccgcgccg acgtgccccg
     7381 gctgcgcgtc tactgctccg accaggcgca ttcctcgatt gacaagggcg tcatccttgc
     7441 gggcttcggc cacgaggcgc tgcgccgcat tccctgcgac gcggagttcc gcctgccgcc
     7501 cgacgcgctc ggcgccgcca tcgccgagga ccggtcggcg ggcgtgctgc cggtcgccgt
     7561 cgtcgccacg gtgggcacga cctcgacgac gagcgtggac ccggtggcgg aagtcgccgg
     7621 catctgcgaa gccgagggtc tgtggctgca cgtcgatgcg gcgtacgccg gcgcggcggc
     7681 gatgctgccc gagcacgcgc acattctggc gggcgcggag cgcgccgact cgctcgtcgt
     7741 gaacccgcac aagtggctgt tcacgccgtt cgacctgagc gcgtttttct gccggcgcat
     7801 ggacgtcctg cgccaggcct tctcgctcgt gccggagtac ctgaggacgc cggaggccgc
     7861 cgccaacctg atggacaccg gggtgcagct cggccgccgg ttccgcgcgc tgaagctctg
     7921 gatgctgctc cgctacttcg gtgccgacgg cctgcgcggc cggatccggc atcacgtcgg
     7981 cctcggccgg cagctggcgc gctgggtgga cgcgcacccg gatttcgagc gcctcgcgcc
     8041 ggcgcccttc agcgtcgtct gcttccgcgc gcggccgcgc gggcgcgagt ggagcgaggc
     8101 cggcctcgcg cgcttgaacg aagcgatcct ggcgcgggtg aacgcctcgg gcgaggtctt
     8161 catctcgcac acggtgcttc acggccgctt cgcgctgcgc gccgcgatcg gcaacctgca
     8221 gacgaccgag cggcacgtgg cgcgggcatg ggagctcgtg caggaggcgc tcgacgcgga
     8281 gctggcctga ctcgggcgct cgggacggcg gtgccacctg tcgcgaaccg cgtctctaca
     8341 gatggaccgg aatgcggcgc gcgcgggtgg cgcccgccgg cgtcatgtcg agggcgcagg
     8401 ccaggacgag gacgccggcc gccgacgccg cgcggatggc cgcgccaaaa tccgggtcga
     8461 tgtcgtcggc cggcgccact gacgcgacat cgccgcgctg ggccacgaag acgatggccg
     8521 cgcggcggcg cgcgcgcgcg agcgcggcca gctcgtcgca gtgccgcgcc gcccgcgcgc
     8581 tcacggcgtc gggaaacgcg ccgacgcgcg ccgtggcggc tccacgatcc gagccggcgg
     8641 cggcactccg tcgccctgcg cgcgccgccg aacgggtcgc ggcgagcgtg accgacttca
     8701 cttcgaccag cgtcttccca agctggaagt cgaaccgcga tcggccgtgc gcgacctccg
     8761 cgcgcacagc gccgagacga gacccgagct ccgggaaaag cccgtgcgag acgagcgcgg
     8821 gaaagacccg gttggcgtag agggtgttcg tgccgatcca gccgtcggca ggcctggcgg
     8881 ccagcatcgt gtagagcagc gagcgcggcg gcgggaaggg cccgtccagc agcacggtgc
     8941 accggggcgc cagcacgccg gtcagccggc cggggttggg cacgtgggcc gcgaccacct
     9001 cgccatctcg cctccggcag atcgcggcga accggtgcgg ccgctcgagg aacgtcgcca
     9061 cgagcggccg cgcgcgcccc tgcgcgagcg gcggcgcgtt gacggggatc cacccggggt
     9121 caggcccggc cactacttcg ccttcggcgc ggcgccgcgc ctggccagcg cgagcagggc
     9181 cttcggatcc aggagttcgc cggcccggac aacgtgcgtg atggccgaga ggtcctcgat
     9241 gcgcgtcagc gggtcgcccg tcacgacgaa gaagtcggcc ggcgctcccg ccgagagccc
     9301 ggcgcctccc gtctgtgcgc cgacgagcgc cgagcctgcc gccgtcgccg cgcgaattgc
     9361 ctcggccggc gtcagcccgg cgcgcacgaa ggcggcaagt tcgcggtgca cgccgccgcc
     9421 gggaacggga tagccgtcga attcgaaacc ggtgccggcg gctacgcggc ctccggccct
     9481 gacgaagcgc acgaggaacg ccgcctggct cgcccacgct cgccgcctct tcggggcgtc
     9541 ggccgccgtc cggctcaggc gtgcctccag ggcggcgcgg cgcgcagccg gaaggagcgc
     9601 catcgacggg tccttcagcg cctcttccgg gaacgctctg gcggcagcgg ccgccatcag
     9661 gggcacgagc gtgacccgtc gccgcagcag gcccgcctgc agcgccgcga gatccctggc
     9721 gccgacctcc ggccacggat cgtctgtcgg gcccgcggag gctttcagcg ggaacgcgag
     9781 cgtctcgatc gacgcgacgc ccgccgcagc caggtccgcc atcgacgcgg ctcccgggcg
     9841 cccgctgact cgcacgccgg cctgccgggc ggctgctgcc agggcctggt acgactctgg
     9901 ccccagcgcc tcgtagcccg cgatccagtc gaccttcgcg gcgacctgcc gcgcggcctc
     9961 ggcggcggcg gcccgggcgt ccggcgcgtc gaacagccag cggtcggggc ttgcgccctg
    10021 gttgatgccc cgcccgctcg tccaaagccg cggcgccagc acatcgcccg cggcggccct
    10081 cgcccgctgc gccacggccc agctcgtgcg cgcgttcagg acgcgatacc cggtcacgcc
    10141 gtgggcgagg cccatcaggt agaagtagtc cgcgtcgaga tcgggcgccg gcgcgcccca
    10201 tgcgtgcgcg tccagcagcc cgggcacgac cacggccccg tccgccttga cgtgcgccgt
    10261 gacggcgggc atcggcgacg gcgggcccgg cgtcacggcc gcgatgcggt cgccctcaac
    10321 gaggatggcg gcgggagcca ggtagcggcc gcgcgccgcg tccaggatcc gcgcgcctgt
    10381 caccagcacg acgggcaccg gcggcggctg ctgacccgcc agaatcgcgc ccatcgcgag
    10441 tgcggccgaa gcggccgtcc ggacggccgt gcgacagttc atgctgccga cagtctagcc
    10501 ctgaaccccg ggccgcggag cccgaatcaa aagggcctgc cggcaagccg gccagccagc
    10561 ttcggcgggc gagcgttgcc acttgtcgcc tgccccttgc cccttctatg atgcagcgtt
    10621 cgcgcaaggc tgccatcatg cgagaaattg tcgtcacccg ccacggctcc cccgaggtga
    10681 tggagattcg ggaacgcccg atccccgaac ccgggcctgg cgacgtccgc attcgagtga
    10741 aggccgcggg cgtgaacttc gccgacgtgc tggcgcggat gggcttgtat ggagacgccc
    10801 cgccgcttcc tttcgtcccg ggctacgagg tatccggcta catcgacgcg ctgggccccg
    10861 ggccgtcgcg ccacgagcgg ggccgccacg tcgtggcgct cacccccttc ggcgggtacg
    10921 ccgagtacgc cgtcgccgcg tcggacttcg tgtggaacgt gcctccgccg ctcagccaca
    10981 acgaggcggc cgccatgccc gtcacgttcc tgaccggctc ggtcgcgctc taccggatgg
    11041 ccaacttgcg gtcgggcgag agcgtgctcg ttcgaagcgc cggcgggggc gttggcgtgg
    11101 ccgccgtgca gctggcgcgg ctgcggaggg cgacggtcat cggcacggcc tcttcggcca
    11161 agcacagcgc cctgcgcagc ctgggcatcg accacgtgat cgacgatcgg cgcgcggacc
    11221 tggaggcgga gatcgcccgc atcacgaacg ggcgcggcgt ggacgtggtc ctggacccgc
    11281 tgggcggcgc gtccctggag cagagttacc gcctgctcgc ccccctcggc cggctgatca
    11341 gctatggcac gcagagctgc gtggacggct tcgagcgcga cgacgcgcgc gtgcagcgcg
    11401 tcctcgacag ccgcccgcgc ttcgatccgc tcgatctgat gcgcgccaac aagggcgtgt
    11461 tcggcctcaa cctcgggcat ctgtggaagg agcgccgcta cgcggcctgg gcgatggagg
    11521 cgctgctcac cgatttcgcg ggcaagcgcc tgcgggccgt cgtgtcgaag acgtttcccc
    11581 tggcggcggc ggccagcgcc cacgcctgcc tgcaggaccg tgcgaacgtg ggaaaggtgg
    11641 tgctgacgat ctaggggctg gcaggctggg ggacgggcag gccggcgcct acctggccgc
    11701 cagcgtgacg cgggcgacgg cgccagccgc caccctgacg agctttcgag gcccgatcgg
    11761 ctttccccgg gcatcttcca gctgcacctg atacgtgccc ggcggcaggc gcgccgcgcc
    11821 gaaccagccg gaggcgtcgc tggtgatcgt gcgcgtctcg tggggcggcc cggcctggcg
    11881 gatgcggacg gtccggcggt ccgcgagcgc gccggcgccg cccgcgatcg agcctgcgag
    11941 cgcgccgtgc gccggcgcgt cgatccacgc catggcggga accggcgccg gccctgtgaa
    12001 ggccgcgggc gcgccagcgg cgccccgcac gaggtagtcg agcctgtcgg gagcgggcgg
    12061 cggcggcgcg ccggcgggct gagcctggga ggcggacgcg gcgccctgcg ccgccgcggg
    12121 ctgctccggc atgacgggct ccatggacgg ctgatagtac gagaacagcg agacgccgtc
    12181 cgcccgcgcc tcgcgcgcgg cgctccgtac gcgcgagatc tgcgccagca cgccttcggg
    12241 cgcgctcagg tagccgccga cgccgacggc cagctggcga tcgcctttgt gcttcgcctc
    12301 ccatgcgatc cagccgtcga accattcgcg cccgcgttcg tcgtgctcgc gcgcgtagtt
    12361 catcggcacg gccaggtcga tgagcccctc ctccagccag ccccgccagt cctgaaagac
    12421 ccgctgcatg ggcgacgcgt tctcgaagtc gcgcgccgac gccggcggcg gcccccaggc
    12481 gacgaccgcc gcgctcaccc tgagccgcgg cttgatggcg cgcgcctcga tcgagatgcg
    12541 ccggacgagg ctggtgatct gttcgcgcct ccaccgggtc cacgcctcgt catccggcag
    12601 gggcgccctc gcgcgcttgt acgcccgctg gaacctcgcg acgctcacct ggttgtatcc
    12661 gacgtccgct ccgcgcggca gcggcgcgcc ttcggtttcg gggtagcgga tgtagtcgaa
    12721 gtggaggccg tccacgtcgt agcgcctgac gatgtccagg cagaccgcga cgaggtggtc
    12781 gcaagcggcg ggatggccgg gatcgaggaa gtacccgact ggaaaccgcc tcgtgccatc
    12841 gcgcatgcgc gtcagccagc agcgctctcc cgcggccgcg ggcccgtgct ggttgaagag
    12901 atggcgtgcg tctttcggcg gcgcctcgtc gcgccagacg ggcatcgcgt tgacccaggc
    12961 gtgcacctgc aggccggcgg cgtgggcggc cgaaacgatg tgggcgagag cgtcgaacga
    13021 agggtcgtag gacggatcgt cgagcggcgg ctcgagaccg cctgtgtaca aggcgtcggc
    13081 ccgccggcgc acctggacga agagcgtgtt gacgtgcgtc cgctgcgcgt cggcgacgag
    13141 ttgcgcggct tccgccggcg agcggatgcc gtggtggaag gcgtccaccc acaccgcgcg
    13201 cagctcgggc ggccccggcc cgtcctgcgc cgtggccccg acggcggcga tcgcgatcat
    13261 catgagggcc gcagcaacgg cccgcacggc cgccgggcct cgcgcgggac ccgggccgcc
    13321 ctggaggggg ccatcgagac aattcgtgca ggcgggcgcg cctgccggtt cgcgcgggtc
    13381 tatcatcatg cgtctctgct tccgggccac acgagggaac cgatgaaacc tgctgcttcc
    13441 gttgccatcg tcgccatgct gactgtcggc gccccgctgc ctgcagcggg cctggccgcg
    13501 ccgcaggcgg ccgcccaggc gtctgcgccc gccgcggcgc tgacgcccgc gcccgttgcg
    13561 ccgaccgtcg atcagctcgt ctccctgaag cgcgccggct ccccggcgat ctcgcccgac
    13621 ggcactatgg tcgcgtacac ggtccgcgaa gccaactggg aggaggacgc gtacgagacc
    13681 gagatctgga tcgccgacgt cgcgtccggc gcggtgcgcc agttgaccaa cggcaggaag
    13741 tccagcgacg cgccggcgtg ggcgcccgac ggctcgcgcc tggcgttcgc gtcgacccgc
    13801 tccgacaagc ggcagatatg gctcatccgc ccgtcgttcg gcgaggcgga gcagctcacg
    13861 cacgagcgcg agggcgtgga acgcttcgcg tggtcgcccg acggacggca gatcgcgttc
    13921 acgcagctgg atccgaagag cgaggcctgg cgggagcgcg acaagaagta cggggagttc
    13981 gaaatcgtgg gcgccgatca ccggatgtcg cacctgtggg tcatcgacgt cgagtcgaag
    14041 aggaccaggc ggctcaccaa gggcgacttc accgtcggca gcttcgactg gtccccggac
    14101 ggcacgcgga tcgcgttcga tcacacggtc aacgcggatc cggccagcag cggcacgtcc
    14161 gacatctcga tcgtcgacgt cgccggcggc gcgatcacgc cgctcgtgag gcaggagggc
    14221 cccgacacgc ggccgctctg gtcgcccgac gggtcgcacg tcgcgttcgc gtcggcgatg
    14281 gcgcggccct tctactacta cacgaacagc cggatcgccg tgatcccggc ggcgggcggc
    14341 gccatcacga atgccacggc aggcttcgac gagaacgcga gcccgctcat gtgggcggcg
    14401 aaaggcttcc tcctcttcag cgccgcccag cgcacgggcg cgcacgtctt cctcctgcat
    14461 ccgtcgtcgg gcgaactgcg gcaggtcacg gcaggcgcgc agcaggccta ttccggcttc
    14521 agtgccaccc gcgacggctc ggcgatggcc ttcatccgtt ccggacccgg agagttcccg
    14581 gaggtgtgcg tcctcacgct gtcgggatcc gacagcagcc tgaagaagct caccagcctg
    14641 ggcgctcaag cggcgtcgtg gcctcagccg tcgcaggagg tcatcgcctg gaagagcctg
    14701 gacggcacgc tcatcgaagg cgtgctccac aagcccgcgg acttcaaggc cggcacgcgc
    14761 caccccctgc tcgtcgtcat ccacggcggg ccgacgggcg tctcgcggcc ggttccgtac
    14821 ggctcgacgt ccgcctatcc catcgacatc tggctcagca ggggcgcgat cgtcctcgag
    14881 cccaactacc gcggcagcgc cggctacggc gaggcgttcc gatcgctcaa cgtccgcaac
    14941 ctcgggatcg gcgacgcctg ggacgtcctc tcgggcatcg accatctcgt ggcgcagggc
    15001 ctggccgaca ccgaccgcgt cggcgtgatg ggctggagcc aggggggcta catctcggcg
    15061 ttcctggcga cgcacgacag cgcgcggttc aaggcggtgt cggtcggggc gggcatctcg
    15121 gactggatga cgtactacgt caacacggac atctacccgt tcacgcgcca gtacctgaag
    15181 gcgacgccct gggacgaccc gaagatctac gcggacacgt cgcccatcac ctacatcaag
    15241 cgggcgtcgg cgccgacgct catccagcac ggcgaactcg atcggcgcgt gcccatcccg
    15301 aacgcctacg agctcctgcg tggcctgctc gatcacggcg tgccggcgaa gctcatcgtc
    15361 tacaagggct tcggtcacgg gctgacgaag ccgaaggcgc accgcgcggc gatggagcac
    15421 aacctcgagt ggtttgggcg ctacatcttc ggcgaggcgc cgcccgccca agcccggcag
    15481 gccgcccgcg tcgagccgtt cctcggcgtc ctcaacttcg cgcggatcga cgaccgcttc
    15541 gccacgggcg gggcgatgaa gagcgaggcg gccgccgagc tgaagcgcca gggattcaag
    15601 gcggtgctca acctgcgcgc cgccagcgag cccggcgccg acgtcgaggc ggcgaaggcc
    15661 gccgtcgagg cggcggggat gaagtacatc cacatcccct tcgtgcgcac cgacccggcc
    15721 gccgacgcgg ccgttgacgc gttcctggcg gccacgaagg accgctcgaa ccagccgctg
    15781 ttcttccatt gcggcggggg gaaccgggcc gctgcgatct gggccgtgcg gcgcgtgatg
    15841 gtggacggct ggccgaggag ccgcgccgtc gcggaagccg actcggtggg cgtcatctcg
    15901 ggcgcgatga ggaagtgggc ggaggagtac ctgtcagcgc acggacggca ggagccagaa
    15961 ggagccagaa gtcggaatcc tgaatcctgg attcttcaga actcgatgcg tacgcctgcg
    16021 accagcgtcc gcccccactg gtgaacgggc ttcggcccct tgagcgcgcg gttccagcgg
    16081 taggtgagca cgttgcgccg gttcaggagg ttgtccacct cgaggtacgc ggcggccgag
    16141 gcgaacctgg tgcggacgct gcgatcgacg cgcacgtcca gccgccggta gtacgggtag
    16201 ttcagcgcgt tgatctgcgt caggtcgtag gcggcccgtc cccgcttcac ggacagcggc
    16261 acgctgaacg gggtgtacgg gcggccgctc gcgtggcgca ggcggaaccc ggcgctccac
    16321 gggccgtccg gccggtagcg cacttccagg cgcgcctggt gccgcagctc gtgctccgca
    16381 ggcctccagg cccggtcgag gccggactgc gaaaccttcc agtaggagta gttcgtcgca
    16441 acctcgagtc ccggcagcgg cgagagctgg gccagcgagt cgatgcccga cgcccggacg
    16501 cgcccagcgc caacgagcgg gccgacgtag ggcggctcga agtccgccgc cgaactgacg
    16561 agcacgcggc tcggcacggc gggatcgacc gggtagttcc ggtaccgctt gccgaaggct
    16621 tcgacggcca ggcgcagcca ggggcgcggc tcgaagtcga gcccgccgcc gaactggcgc
    16681 gacgccatcg gcgccaggcc gatgttgtct ggcgcgctgg ccatccagat gtacggcacg
    16741 ccctgccggt agatgcccca gtggccgacg aggcgcacgg accgcccggc cacgatctcc
    16801 gccttcaccc tcgggctgcc tgcggtcaga gacgccgcgc cccagcggtc gatcctgacg
    16861 cccgcgagca gcctcccgcg ccccgcgatc gcgcgttccg cctccgcgta ggcggcaaga
    16921 tcggtgaagg agcggcgctg acgcgacgac aggtcccggt tcagcgcgtt gtatggcgtc
    16981 cagagatcgt tgacgaacag ctcgtagtcg tagcggtagg tcttgacggc cccgcccacc
    17041 agaatgtcgc cgacgaacgt cctcttgcgg cgcacgtcgg cccggatccg gacctcggtc
    17101 tccttgcccg cgtcgatggc gtccaccacc gccccatcga cggccctggc gtccacgtcg
    17161 atggagctca gactggcgac cacggacgag aaggcccgcg ccgaccacga ggagtcgaaa
    17221 cggacgcccg caagagccgt cgtttcgctg ccggagagct cgtcgtctcc ggccttctcg
    17281 tcgtgcccgc tggcctcgtc gcgggagccg aggccgagca cggtcaggcg gtgtttctcg
    17341 cccgcccgat gctcggcttt cacgagcacg tcggcgtagg ccgggacgat ctcgtcggtc
    17401 agcttgctga agaccgcgtc gaggaggctc cggcgggcgg acaccagcca ggagccacgc
    17461 gcgccgaggg cgccctcccc gatggccgtc agcccgccga tccccgcgcc caccgccgcg
    17521 tgcacgcggt cgcgccgccc cgggcggatg ctgacgtcgg ccaccgacga catgcggtcg
    17581 ccgaaggcca ccgagaagcc gccgacttcc agcgagccgc gctcgatgag ccagggcggc
    17641 agaatcgaca gcgccccgcc gctcccgcct tgcgcgccga agtggttcac gttgggcagg
    17701 tcgaacccgt cgatgcgcat ctggttctcg agcgcgcctc cgccgcgcac gagcagatcg
    17761 ttccggttgt cctgcgaccc ggccacgccg ggcagcgact ggaaggcgcg cgacaggtcc
    17821 tcgagggcgc ccggcgtctc cgcaatcgcc gcgccggtca gcacgggcgc gatggcggcg
    17881 gggatcggcg tcgcgcccag ggcccgcggc aggccggccc gcgcctcggc cagcatctgg
    17941 aaacggaacg ggatgtccgc cgtgacggcg cagggctggc cgtccagccg gacctccagg
    18001 ggcgccgact cggcgaaccc ttcgatcgac gctgtcaggg cgtagtcccc aggcaggaga
    18061 tccgcgaagg cgaaccggcc gtcgccgtcc gcgacggtct ggcatcctcc gcggcacgac
    18121 ggcggtgaag ctgcgtcctg gaggcgctcc ggcgagaagg cccgcagcac gacgaccgcc
    18181 cgcgacacgc ccgcgcgggt ccgctcgtcc acgaccgtgc cgccgacgtg gcaggccggc
    18241 acgaagcgag gcgacggcgc cggcgcggcg ggcgtctgcg cggcgggtgc ctgcgcggca
    18301 acgagcacga cgagcatcga gagcgccgtc ggcatgcctg gcccaggccg cggactatat
    18361 cacccgccct ggcccggcgc gccggaaacc ctgcatgtcg cgtcctgccg cgctgtcaag
    18421 ggattctgca cgctgatatg gctcgaagga gatcgccgcg tacctcgccc gcggcgtccg
    18481 cgcggcccgg cgctgggatc cggctgcggg gcggcacgtc gaggtctccg acgccaggca
    18541 gccgctccta cgccagcgtc ttccgcgatc gcgccgtcgc cagcgccagc acggccgccc
    18601 cccacgccag catcagcagc gcatcgggcc agaacgcctc gaggcccacg cccttcagga
    18661 agatcccgcg cacgatcacc aggtagtagc ggaggggcac gagatacgtc agcggctgga
    18721 tggcggcggg catgttctcg atcgggaaga cgaagccgga gaggtagatc atcggcatca
    18781 tgaaaaagaa cacggcggtc atcatggcct gctgctggtt cctggcgagg gtggacacga
    18841 gcagccccag ccccagcgtg cacagcaggt agaccatcga gaggccgagg agcagcggga
    18901 agctgccgcg cagcggcacc tcgaaccaca gcgcggcgac tcccacgacc aggaacacgt
    18961 cgagcgtgcc gatgaggccg tacggcagca gcttgccggc gatcagctcc cagcgcctga
    19021 gcggcgtgac gttcagttgt tcgagggtgc ccagctcctt ctcgcgcacg acagccatcg
    19081 atccgagcac gctcgtaatc accatcagca ggagcgcgag cacaccgggc accatgaagt
    19141 gcctgctcag cagctgcggg ttgaaccaca cgcggatgcg ggcctcgatc gcccccgccg
    19201 ccggccggcc gcgcgggcgg cgcgccagcg cgagctccga cgccttgtcc gcgatgagcg
    19261 tcgtcgcgta cgtcaggccg aggcccgccg aattggagtc ggtcccgtcg gcgacgacct
    19321 gcagcgtgac gggcctgccc gccgccaccg actcgccgta ctcgggcggg atcgacaggg
    19381 ccatccacgc gcgccgcgtc tcgatgtcgc ggagcacgtc accctcgtcg gaggtgacgc
    19441 gcgagacgct gaagtagggc gacccctcga accggccgat gagctcccgg ctggcctggc
    19501 tgcggtcgcc gtccacgatg gtgatgggca cgttcctgac gtcggtactc gccgcgtagc
    19561 cgaggatgaa gagctgcagg acgggcgcga ggaagatgag cgggaacagg cgcgggttcc
    19621 ggcgcagctc ctggagctcc ttgcggacca gaaacagcag ccggcgcatc agccccgctc
    19681 cctggccagg cggatcgacg ccaggctcat gaccgccgtc acgtacaccc cgagcgcggc
    19741 gaattcgagc gcgagcggcg cgaacccgat tcccttgagg aggatgccgc ggaggatcac
    19801 gatgaagtag cgcgccggca cgatgaaggt gacgtactgg atcaccgcgg gcatgctggc
    19861 gatggggaag atgaagcccg acaggacgag cgtcgtgagc agggagatca gcaggctcgc
    19921 cagaaacgcg gtggcctggg tgtcgaagag ggtggacacc agcagccccg ttcccaggcc
    19981 tcccaggacg aacagggcga ccgacccggc cagcagcggc cacgacccgc gcatcgggag
    20041 gccgaacagc agcatggccg cggcgatgac gaggaacgcc gccgcgagcg agaggaggaa
    20101 gtacgggacg gacttgccga tgatgtacga cggcgtggcg atgggcgcca tccgcacctg
    20161 ctcccaggtg ccccgctcct tctcgcgcac gatcgacagc gccgtggcga cgacgcagct
    20221 gatcatcacg atgaaggcga tgaggcccgg cacgaggaac agtgagctgg ccagctcggg
    20281 gttgtaccag atgcgcggct ccaccgagag gggcgcggcc ggggcgccgg tcgaggcgcc
    20341 gagccggtcg gcagacgcgc cccggacgat ggcctgcgcg tagccgaaga cggtggaggc
    20401 cgtgttggcg ttgtcgccgt ccaccaggac ctgcaccgcg gccgtgcgcc cggccgccag
    20461 gtcccgcgtc atcccctcgg gaatcacgag cgccgctcgc gcctcgccgc ggtccatcag
    20521 ccgctcgatt cccgccatgg acgagggtgt cgcgacgacg tcgaagtagg tggaattgac
    20581 gaaggacgag agcagctgcc ggctctcggc cgtgcggtcc cggtccatca ccgccagccg
    20641 cacgtgccgg acgtcccagt tgagggcgta gccgaacagc agcaggagca ggaccgggac
    20701 gagcagcagg atggcgagcg tgagccggtc gcgcaggacc tgcgcgagct cttttcgccc
    20761 gaccgccagc gctttcctca tgcagccgtc ctttcctccc gctcgacgac gtccatgaag
    20821 acgtcctcga gcgatggctc caccaggtcg agcgactcgg cctcgattcc cgacgcgcgg
    20881 agccggtcgc gcaggggcgc cacgtccgtt tcgccgggcc tgagccagac gtgcagcgcc
    20941 gtgccgaacg ggctggactt ctcgaccgac ggcacgccgt ccagcgcgcg aagcgcggcc
    21001 atcggcgacg tggtgcgcac ctcgacgacc aggcggccgc tgaagacccg cttcagctcc
    21061 gcgaccgtgc cgagggcggc cagccgcccc gcgtgcatca gcgccaggcg gtggcagtgc
    21121 tcggcttcgt ccaggtagtg ggtcgtcacg aagacggtga cgccctggcc cgagagcccg
    21181 tcgatgagcc gccagaactc gcggcgcgaa accgggtcca cgccgccggt gggttcgtcc
    21241 aggaacagga tgcgcggctc gtgcaggatg gcgcacccca gggcgaggcg ctggcgccag
    21301 ccgcccgcca gatcgcgcgc gagcgtgcgc tccaggcctt cgaggcccgc catcttcagc
    21361 acgtacgcgc cgcgcgtgcg cagggcgcga tcgtcgaggc cgtacatccc gccgaagaac
    21421 ctgatgttct cctgcacggt gagcagctcg tacagcgaga acctctggga catgtagccg
    21481 atccgctgct tgacctcctc ggggtcgcgg gtcacctcga tgccgtccac gacggccgac
    21541 ccggacgacg gcctgaggag cccgcacagc atccggatcg tcgtcgactt gccggcgccg
    21601 ttgctgccga ggaacccgaa gatctcgccc cgtccgacgt cgaaggagac gcggtccacg
    21661 gccaggaatc ggccgaagcg gcgcgacaga tcgcggacgg tgacggcggg agcggacaag
    21721 gaggagggca tgggcgctct acctccccac cgcccggtcg aggcgcgcgg cggcgaggcg
    21781 cgcggcggcc agcgcgcgcg tgcggtcgaa ctgcgccgcg agcagttcct gctgcgcgtc
    21841 gagcacctcg gtgctcgtca cgaggcccgc cttgaaccgc tcggccacga cccgccgcgc
    21901 ctccgccgcc gaggccacct cgtcggtcgc ggcgcggatc gacgcgtgcg ccgattcgag
    21961 gtcgagccgg cgctgtctga cctcgaaacc aagcgccgcg tcgaactccg ccagccgctc
    22021 ggccagcgcg cgcctggaag cggcggcctc ggcgatttcg gctttcgccc gtcccccgtc
    22081 ccagagcggc cacgccacgc tgacgccgag gtcccaggaa tcgttccaca ccgccgcccg
    22141 cgggaagatg cgcgcgttgg gcctggcgta gtcgacgccg gcggtcagcg ccacgctggg
    22201 gcgccgtccg gccgcagcgg ccgcctggcg ctcgccgacg gcggccgctc gcgactcgag
    22261 ggcctggcgc tcgggccgct tcgcctgcgc ctccgccagc agggcctcgc cggacggaat
    22321 gggagcgggc gcggcctcga gcgcggcgtc gacggcgatg gacacgtcgg gcgggatgcc
    22381 cgtgacgcgc cgcaggtcgg cctccgccac gtcgcggatg gtgcgcgcct cgatcagctg
    22441 gacctcctgc cgggaccggc gggcctcggc gctcaggacg tcgttcggcg cgagaaggcc
    22501 gacggccctc atgttcctca cgtcgcgcag gtgcgcgtcc accagggcca gcgcctcctc
    22561 caccacgcgc acggactcga tggccgtggc gtacgcccag aacgcgcgcg cggcgtcgag
    22621 gcgcacgtcg gccctggttg tcgccgtgtc cctgccggac gcggcggcct cggcgcgggc
    22681 ggcccgctcg gcggcctcga cgcgcccgaa cgtgtagatc ggccactgca cgtcgagacg
    22741 cgaacgccag ttgtccggaa catcggggta gatgatccgc gcgggcgcgg cccccatggc
    22801 cgggatgccg aactcgttca cgtggttcgt gcgcaggtag ccggcggcga ggctgattgt
    22861 gggcatgttc gctgccttgc gcgcggctac ggccgcatcg gccgcctcct ggcgggcgag
    22921 ggcctcgccg atgcggtggc tcgcctgcag agcgcgggcg acggcgtcat ccagcgagag
    22981 cgtgacggcg ccggacggcg ccggaggcgt tgccggcgcc ggcgcggccg ggggcggaac
    23041 gggcgggggc ggaacggcgg tcgacgcggc ggcgccgggc ccggcggcgc cgagcgtggc
    23101 ggccagagcg gcaatcagga aggcggcggc ggtcgggcgg cgtgcgtgat cgttcatgct
    23161 gcaccctggt cgggcgtcgt gacgacgcgg tcgatgaaga catcttcgag ggacgcggga
    23221 accgtgcgga ggccggtcac gtcgatcccg gcctcgcggc aggcggcgcc caggcgccgc
    23281 gtgacgccgg ggccttcgcc gcgtggcacg cgcacgtgca ggcgctcgcc gaagcgctgc
    23341 acgtcgcgca cggccggatc cagcagggcg atccgcgcgg cttcatcctg gcgcggcgcc
    23401 agcatctcca cgaggtcgtc gggaaaggcc gcccgaagcg agcccggatc gtccagcgcg
    23461 agaacccgcc cctggctcat cagcgccacg cgcgagcatc gctccgcctc gtccaggtag
    23521 ggcgtggcca tgaagatcgt gatgccctgc gacaggaact ccgacagcag gtgccagaac
    23581 tcgcggcgcg acaccgggtc cacgccggtc gtcggctcgt cgagcaggat gacctccggc
    23641 tcgtgcacga gcgtgcaggc cagggcgagc ttctgcttca tcccgcccga cagccgctcc
    23701 gccagccgcc ctctgaacgg cgtgagctgc atcatgtcga ggaggcggtt ccggcgctcg
    23761 cggtagtcgg ctacgccgtg gatctcggcg aagaaggcga tgttctcgtc gatgctgaga
    23821 tcgccgtaga ggctgaactt ctgcgagaag taaccgacct tccgcgtgat ggcgccgtgc
    23881 gcccggacgg ggtcctggcc caggacgcgg atgctcccgc tggaggccct cagcagcccg
    23941 cacagcagcc ggatggtggt cgtcttgccc gcgccgtccg ggccgatgag gccgaacatc
    24001 tccccgggtt ccacggcgta cgagacgtcg cgcaccgcgt cggtcgggcc gaagcgcttc
    24061 gtcacccgtt cgagctccag tgccagtgcc attgctgctg tcccttgtcc ccgctttcgc
    24121 ttgttcctgc cttcgctggg cggcctgtcg atccgccttc tgcattctga attctggatt
    24181 ccgtctcgtc ccatgcccct gtccgacgag cgcctagcgc ccagcccctg gcgcctgctc
    24241 tttgagcgga atctccgcct ccaccggcat tccctgtttg aggacgcccg accggttgtc
    24301 gaccgtgatc ttcacgcgga acaccagttt cgaccgctcc tcggccgtct gcacgttgcg
    24361 cggcgtgaac tcggccttcg gcgagatgaa cgtgaccgtg ccgtcgatgc ccgggccccc
    24421 ggcgtcggtg aagaccctgg ccgattgccc gagggtgacg cgcgggacga ggggctcgtc
    24481 cacgtagacg ttggcccaga cgcggtccag gtcggccacg acgaggatcg gggtgcgggg
    24541 agcgatgagc tcgccggcgt ccacgagctt ctgggtgacc acgccggccg ctgaggcgag
    24601 caccgtggcg tcattgaggg ccttgtcgag ggtggcgatc tgggcgtcgg cggcggccag
    24661 gcgggcgcgg gcggcgtcga tctcttcccg ccgggcgccg gcgcggagcc tggcgacggt
    24721 ttcggcagcc gccctggccc gcgcttctgc ggcgcgcact cggtcgcgcg tcacgtcccg
    24781 cctcgtctgg gcgtcgtcgc gcggtttctc ggcgccggag ttggcggcga ggagggtgtc
    24841 gaagcgctgg aggtcgcgtt cggccgatgc gagctcctgg cgtgctgccg ccaggtccgc
    24901 ttccgcggcg gcgtgctgcg cttcggcctg ccggatgtcc tccgcgcgcg cgccggcctg
    24961 gagcaggcgc agctgggcgt cggcggcctg gcgctctgcg gcggcgcgcc ggagggccag
    25021 ggtgacgtcg gccgtgtcga gctgggcgac tgtgtcgccg gccttcacgc ggtcgccctc
    25081 gtcggctctc agctcgagca ggcgcccgcc gacttccggc gctatgcgga tctccgtggc
    25141 ctcgacgtaa cccgagaccc ggacgtggtc ggccgcgggc tcgtcgcggc aggcgagtga
    25201 ggcggcgagc gtggcggcgg cggcggcgac ggcggccgcg cgggcgagtc gggcggcgcg
    25261 gggttcgcgg tagctcatga cgttactccg gtcgggatgt cggcggcggc cgcgggcgtg
    25321 agggcgcggc ggttcagcgt ctggaggtgg aggatgaagc ggtcgggatc gagcctgcct
    25381 ggctcggcgt ggctgatgcg ggcgaaggcg tgcctgatcg ggccggaggc gaggtaggcg
    25441 acgatgggcc agatggtggt cagggcgagg aggagcgggt cgacgggggc gaaggcgccg
    25501 tcgcggcggc cctggtcgag gatggagcgg aggatgccgg cgacgcgggc cgtgacggcg
    25561 agggtttctt cgtcgaggcg gcggccgccg gaggcgagtt cgcggagcat gatgggagcg
    25621 aagtgggcga ggcgctggcc ttcgaggacg aaggcggaga cgaagcggtc gagcctgacc
    25681 tgtggcgggt cggtcgaggc ggcggtggcg gcgaggcagt ctcccatgag ggtgaagagg
    25741 gtgcggagga cggaggtgta gagggcgcgc ttgctgcgga agtggtagta gatcatcgcc
    25801 ttgttgagtc gtgcgcggcg ggcgatgcgg tcgacggtgg cgggctcgaa gccgcggagg
    25861 gcgaattcgg cggcggcggc gtggaggatg cgctgccggg aatcggaggg cggaaggggg
    25921 gtggcgggcg accggcgggg gcccggagtg cggcgtcgcg gggtcttcac gctcatacta
    25981 accaaccagt taatctaact aactagttag tatcataatg ccgccgcggg cgtcaagccc
    26041 gcgcccggag cacccccgtc ggcccgcccg ccgcgccgcc cggaccacgc acgcagatct
    26101 cccgccggga ccccgcctcc caacgccaga atctcgagcc ccgcgcccga ctttccacta
    26161 tccttccccc atgcgcgtcg tcatcgccgg cggctccggc ttcctcggcc gcgccctcgc
    26221 cgcgtcgctc gcccgcgacg gccaccgcat ccaaatcctc acccgccgcc gcctgcgcca
    26281 ggccccgcct ctcccacagc ctcccgccga cgcttcttcc ccagccgccc ccgcacccgt
    26341 cgaatacatt acctggcagg ccgacggcag cgtcggcccc tgggccggcc cctgccgcgg
    26401 cgccgacacg atcgtcaacc tcgccggcga gtccatcggc agccgccgat ggtccctcgc
    26461 ccgcaaagac gccctcgtcc agagccgcct cctcgcgacg cgatcgctcg tgcgcttcat
    26521 ccgccagtcc agccccccgc cctcggcgtt cctcagcgcg tcagccatcg gcctctacgg
    26581 cgaccgcggc agcgatatcc tcgccgaaga cgcggaaccc ggatcgggat tcctcgccag
    26641 cctcgccgcc gcctgggaac gcgaggcccg cgacgccgac ggcggaccca cgcgggtcgt
    26701 cctgttgcgg acgggcatcg tgctcgatcc ccgcagcggc gccctcgcgc gcatgctcct
    26761 gccctaccgc gtctgcgctg gcgggcccct cggcccgggc cgccagttca tgtcctggat
    26821 ccaccgcgac gactgggtgt cgctggcccg ttgggcgatc cgcacgccgg aggtgcgggg
    26881 cccgctcaac ctgaccgcgc cgcacgccgt cacgaacgcc actttcgccc gcgctctcag
    26941 gcgcgtgctc cgccgcccgt cgctcgtgcg cgtgcccgcg ttcgccctga agctcctgct
    27001 cggtgaaatg gcggggccgc tgctcttcta cagccagcga gtcgtcccca ggaaggcgct
    27061 ggacggcggg ttcaccttcg cgcacccgga agtcgaaggc gccctggccc acctgctgga
    27121 ctcgaaagcg gcgcccgccg cctgagacgg tccccggccg cagccggcag gggacgctag
    27181 acgaacgcga gcccgggccg ccggaagtcg ccctgaagga tcgacaccga gtcgccgccc
    27241 agccagtgat cgatcacgcg cgcgtaaacg gaccggaagt ccgtctcgta gccgacgtcg
    27301 ccgccgctgt tctccagggc cgggttgtcc ggcgcggggt tgagcgaggc ggccgtcccg
    27361 tagagcccgc cgcgcaccgc gccgcccgcc gcgaacatca ggcccgccgc gccgtgatcc
    27421 gtgccggcgc tgccattctc gtacgcccgc cgcccgaact cggagaacgt catgagcagg
    27481 acgtcccccc acagcccctg cgcccggagg tccgcgtaga aggcgaccac cgcatcgttg
    27541 aactcggtca tgagccgcgg gtacgtcgcc gcctgcgccg cgtgcgtgtc gaagccgccg
    27601 agctgaaccc agaacacgcg cgtccccacg cctttcacga gcgccccggc cacggtcttg
    27661 agcgtcgttc cggtcggagt cgagggatag acggcggcgg gcgcgtactt cgccacgaga
    27721 gccaccttgt cgagcgtgtt catggcggcg tcgatgcccg cattgacgaa ggccaggtgc
    27781 gggcggtccg acgcgtcgcg cgatgccagg cgctcggcga agacgcgctc gcgggccgcc
    27841 tccgcgccgc cgttcacggc gctgaacacg tagcccgacg ccgaggggat ggccggcacc
    27901 ggcacgtggt cgctcgtcag cgcgcgcggg atttcgcgca ccgcgtccca cgccgcaagc
    27961 gggtccaccg gctgcggcag cgtgtcaagg tagcgcccga gccacccgtg cgactgcgga
    28021 ctcgcggggt tggccgatcc ccagacgtcg gtgccgagga agtgcgaccg cgtggagttc
    28081 gcgtatccgg tgcgctggac gatcgcaagc cgcccttcgt tgaagatggc gcgcagggcc
    28141 ggaagtccgg gatgcaggcc gaggctgacg ccgctcgaat cggacccgac ctggagcgcg
    28201 tcgcccgccg ccacggcgat ggacggccgc ctgctgtagt agtaggggtc cgtatagggc
    28261 acgagcgtgc tgagggcgtc gttgccgccg gcgaaaaagc acaccaccag cgcccggcgc
    28321 ccggagccct gggcacgcgc caggtcggcc aggaccgccg ggccggccag cccgacggtg
    28381 aacgccccgg caccgtggcg gaggaagtcg cgccgcgtca tcgccatgga atcacccccg
    28441 cgggaaaccc tctcgcggcc gcccgcgccg gcctagacga acacgtactc gccggatccg
    28501 acgatgaggc ggacgagccc cggcaccttc atggccagct gcgcgtccga gcccgtccac
    28561 gcgccgctcg cggtgaggta cgccagcagg tcgtcgtagg cctccctgcc gaagggcgcc
    28621 ggcgacaggc gcccgatctc gtgcgagagc accgcgccgg gcgacgaggc gtgcggccgg
    28681 gcggccttca ccagggccgc ggcctgggcc cggctgagct gcgccgcgaa attcatccgc
    28741 gccagcatgc cgccagacga gatccagccc ggcccctgct cccagccggc cacatccgga
    28801 ggctcgtaca gcacctgccc catgctggcc atctggctga cggcgttgtt gagcgtgaat
    28861 ccctcccacc cgacctcctt caggctccgc gccacgaatt cggcgggcca tgagtacttc
    28921 gtgtggtagt tctcctccga ccggaactcc ggcgactgca gcaggcgccg catcacggcg
    28981 cggaggtcga agccgcgcgc gaggacgtcc cgcgcgagcc cgctcacgaa ccgctcggtc
    29041 ggcggattga cctcgctgac gaagaagctc cagaaccgcg agaccagccg acgcgcggtc
    29101 tgggggtggg ccaccaccgc cgcgatcaga tcgaggccgt cctggagccc tccgtccgcc
    29161 gacctggcgg gaatcgttct gcccccgtcg ggatagattg cgaagctgaa cgtcttggcg
    29221 gccgtgtcgt gcaacgacgc gttgtaggcg aactcgtatc gcgggtagga cggattcgcc
    29281 gccgtgcgcg cgaggttcca gccggtgaag accctggccg cggcgtacac gtccggctcc
    29341 gtgtagaagc cgacacccat gctgaacagc tccagcagct cgcgcccgaa gttctcctgc
    29401 ggcctggcct tcgtgttgta gcgcccgtcc agccagatga gcatcgccgg gtctttcgcc
    29461 atcgccacca tcaggtcgcg gaagttcccc aggcagaggc ggcggatcgt ctcgacctgc
    29521 ccctcctggc cggcgggatc ctcggccgcc ctggcggcca tcaggcgcgt cgcgtcggcc
    29581 gccccgtacg ccccggcgat cttggagtag gcggtggcga agtggttgtg ccagaagagc
    29641 gtcatcttct cctggagcgg ccgcgccgtg tggatcatgc ggaacagcca ccgctgccgg
    29701 gcatccccaa tgaccgtgtt gggcgagaac acggcgccgg cggccttgac gccgacgtag
    29761 cccggcgcgc ccacgaaggc gtctacatcg tcatggatct gctcgaagtc aacgagcgtc
    29821 tcgaccgtgc cccggcgccc cagccggctg tagtactcca gttcggcgtc gctcgcgccg
    29881 aacccggccc ggcggagcag gtgccgcagg acctcgtcgc gatagaccat ggtcgggatc
    29941 tccagacgcc gcgggtcgat tcgccccggg ccggcatcga gctgatactg ccgttggatt
    30001 cgagcgccgc cgccggtgtt acacgccgtc gaggcccggg tcggccgcta ccgcgccggc
    30061 cgcccggctg gcaccggggc gtggcctgcg ctcaggaggt tcgccagcag ctggtaagcg
    30121 ccctcggtcc ccgccggcag ctgccgccag agcccgaggc cgatgtagat ccagcggccc
    30181 ttgcctactt tcgcctcgac cagcgcgccg cgcttgggcc ccttgttgta cgggaagggg
    30241 tcctctgact cgaccagatc caggtagcgt ccgtccttct cgccgaggaa gtagaggccc
    30301 cgttcctgca cccagtcgcg ccaggccgcc tcgccaatcc ggttcggcca cccgaacacg
    30361 gggtgaccgg gctcgaggat ccgaaccggc gcgcgctcgt cggtgacgcg gttcgcactg
    30421 actttggcgg gaaacggccc gtactgggcc tcgttgaact cgaacttgtt gtattgcacg
    30481 agcacggtcc cgccgtcttc cgcgtagcgc aggagacgat ggttgttggc gcggagatcc
    30541 ggccgccgct cgtaggcgcg cacgccgctc acgatgacgt cgaaggcgga gaggtcgccg
    30601 aaggcgagcg cgtccgcatc caggagggtc acgcgcgcgc ccagctgctc gatggccgca
    30661 ggcacctggt cgccgacgcc catgatgtac ccgactcgga ggcggggcgc cacggccacc
    30721 tccacgatct tcacggcggc cgcggcctcc ttcagcaggt gccggcgccg cgtgtggggg
    30781 tactcgatca cctggtagcc ggtcgtgaac cgctccgcgc ccgccgaggc ggcggcgcgc
    30841 acggtgtacg ggcccggctt cgcatcccgc gcgggcgtga cggtgaatcg cacgggcgcg
    30901 gcttcgtcct ctcgcgagaa cgacacgcgc gccgacgcgg gcttgaccgc ccatccgtcc
    30961 gggatctcga gcgagaccgc ggcctcggcc ggccccctgc tgccgttgat gacggtaacc
    31021 cgcacttcac ggctggcggc aggcgcgcgg cgcgcggcgc cggcctgcgc cggagcccct
    31081 ccggccggca cgatggcgac ctcgggcgac acggtcaccg agaagcgcgg cacgacgagc
    31141 aggtccatgc gcttctcgcc gctgaagatg ttcccttcgt accggtactg caccggccgc
    31201 tcgaccgaca gcgccgcgcc gcccacctgc aactcgaacc ggacgcggaa cggcgtcggc
    31261 cggaacggca cgccgaacgg cgcgtcggcg tcgaactggt accgcgcgcc cgtcggcagg
    31321 cgccgccagt gctggccgct cagccgggcc tgcccggaca ccgcgagcgg cgcgtcgcag
    31381 cggtagacgc cggcgggcgc gattgaaccc gtcctgcaca cgccggcttc tccgtcgagc
    31441 ccgcggaatc cagtctgcgt caggacgacg gcttcccggc ccccgtttgc cgcgatgagc
    31501 gtcaccgtga cgggctgacc gggcacgacg aggccgtcat ccgcgagcgc gtcgagccgc
    31561 acgcccgagg cggcgagcag ggcgtcggtg aactgctctt ccttcagggc gagccgggcg
    31621 tcgatctccg cgctcgcgtc ctcgggaagg ccgaacgcgc gcgaggcaag ctgcgcccgg
    31681 agcgcgcgca gcgacgtcag gcccgcggca agcggcgcga cggcggccgc cgggccgccc
    31741 gacgcggcct gcgccgccgc gtccgaaatg cggccggcca ccgaagccag cgccgaggtc
    31801 aggccggccg gggcctcgcc cggcacgaag cgcgcgaggc cggcgagcga ggtgtcgagg
    31861 ccgtcgaaga gcgacgcctc gtccttcccc ttcaggcccg ggatggcgct ctcgacgagg
    31921 gtgaagcgga cggcggccgg ccccggcaac cagaacagct gcgccatgcc ctgggtcttg
    31981 tgatagctgc gggcgcggct gccgatgtcg gcgaacgact ggccgagcag cgggtcgaag
    32041 gccgccgtgt ccaccgtcgt cagcttcgcg ccggctgccg gagccggctc gcccgcgaac
    32101 ccgtactgac ccgtgaagta gaacttcttc ggctgccacg cgcgcaggcc ttcccgaagc
    32161 tgctcgggga acctcgccgg gtcgcccgcc gccaggtacg cctcgcgggc gatcctggcg
    32221 gaagcctggt ggtgctgccc gccgccttca ccgtcgggcc tcatcgccgc gatcacgtcg
    32281 gggcgtgtca tccggatgag gcgcacgaag tcgccgagga tctcctgccg gccccagagg
    32341 tcgagcgtct cgtccacgct gaacgagtag ccgaagtcca cggcgcgggt gaagtactgc
    32401 tcggcgccgt ccagggcgtg cactgagacg agctcttcgg tccgcgcgac ggccagcgcg
    32461 tcgaagagct ccgggccgat ctcgttctgg ccgccgtcgc cgcgcgtggc ggtcgccacg
    32521 atcgtgcgga cgccctgccc ccagctgagc gccgccagca cgccgttcgg ctcgtcgtct
    32581 ggatgcgccg tggcttccat gaacaccgca gtcgtccgga gctttcgcag cagcaggccg
    32641 agtccgacct gcccctgcgc gtcggcgatg ggcggcatct gggccggcag cgcgccgccg
    32701 gccgcgaagg cggcgagcag cgcggcggcg aggacgatcc ggctccgctt catattggct
    32761 ccccggttcc catgcagggg gccgggcccg gccgccgacg tcgcgccggc gagaggggac
    32821 tgccgcccgc cctggcagga gaagacgcct ctcagtgtag tccgtcgcgg cggcgcggtg
    32881 aactggacgg ggcgaggctg gagggcggcg cgacgcctac cccagcaggc gcgtgaagcc
    32941 gaagagctcg tcgccgttgt ccggcgcgcc ggcgtagcgg cgcagcagga cgcgggccgc
    33001 gaggagcgcc gaggcgacgc cgttgtggcc cgccccgagg gcgaagagat gccgcgggta
    33061 gtggcggtgc ggcccggcgc aggggaaccc gtcggccgac ttccacacgg ggacggtcca
    33121 cgcgtattcg ggctgggtgc cggctatggc gggatagagc ctcgacagct cgtacatcaa
    33181 ctggccggcc cgccggacga gcgccttctc gagggtgcgg gccggaagcg ccggctggtc
    33241 cgcccccacg aacaggacgc gctcgtcgct cgtccgccgg aggacgtgcg gcgggtcgtc
    33301 ctgatcgatc acgacggcgc ggctgcgccc gaactcgcgc cgcacgaacg acggcgccgg
    33361 cggcgtcagc acgcaaaagg cgtgcatcgg gctgaagtgc cgccgcagcg gcttgaacgg
    33421 cgccgccggg ccgttgctcg cgacgatgat ggccgcggcc cgcacgatgc cgccgtcggt
    33481 cgcgacctcg acgcctttgc ggcccgcgcg gacgcgcgtc accgtcgtgc gctcgaacac
    33541 gagcgcgcgc cgcttcgccg cctcggccgc cagcccgagc gtggcgcggt acggatcgat
    33601 gaacgcgtcg ccggccgacc ggatggcgcc ctcgcccgag agccccgtct cctgacgcag
    33661 gcgcgcgggc gtcagccacc cgccttccag tccggcgccg cgcatcgccg ccagctcgcg
    33721 ccgaagccgc gccccgtcgc cctcgctcag ccccgcccgg agggccgcgt cgggcaccag
    33781 ttcgcagcgg atgcggaggc ggcggatcgt cgccgccaga tcgaggcccg cgcggcggaa
    33841 cgactgccac agatggcggg ccgcgcgcag gccgtaggcg tgcttcagct cgaggaaggg
    33901 cacggccggc tcgagccgga gcaggcccgg gcacgaggcc gagccgccct ggccaatccg
    33961 gttcgactcg atcagcgcca cgcggacgcc ggacgccgcg aacacgtacg cggccgaaca
    34021 cccggtgagg ccgccgccga ggatggccac ctgcacgtcc acatcgccgc ggtggcgcgg
    34081 gtaggcgggc tggcgggcct tcgggtaccg atcgagccag taggagacgc cgtatcgggt
    34141 tcgcatggtg ccgactgccg gtcgcggagg attctactct gacagcggcg ggaatagaat
    34201 ccgtcagttg cacacggagt gggcggcgac gaggcggatc ccggccggga acgacacaca
    34261 cgaggttgga tgctcatgca caccagacag acgcctggcg tcgtctcccg ggagatcgcg
    34321 atccacctgg aacggcacgg atgcccggcg catgccgccg gcgcgcagcg ctacttcaag
    34381 gacgaagtga agtgctgggg ctggcgcacg aaggacctcc ggtcatacgc ccgcgctgtc
    34441 cacgcggcgc tcgcgcccga tcctgagctg ctcctgaacg tcgccgagcg gctcttcgcc
    34501 gaggcctcgc tcgaagagaa gggcctcggg gtcctcatcc tgcagccgtc gatgaagcgc
    34561 ttcggcggca gggagttccg gcgcctcgag ggttggctcg accgcgtcgc cacgtgggcc
    34621 gaccacgacg cgctgacgat gacgctcctg gggcccatga tcgtcgccga tccgcggcgc
    34681 gtggcccgcc cgctgcgctg ggccgcgtcg cgggaccgct ggcgcaagcg cgcgtcggcg
    34741 gtgtcgctca tcccgggcat ccggcagggc ctcttcatgg aggagggccg gatcgtggcc
    34801 gatcgcctcg ccgccgatcg cgacgacatg gtccagaagg ggctcggctg gctcctgcgc
    34861 gagtgggcga agcaccaccc cgccgaggcg atcccggtgc ttgccgcgat ccgcgggcgc
    34921 gcctcgaggc tggtgcttcg caccggctgc gagaagctgg gcgcggcgga ccgccagcgg
    34981 atcttgcgga agtgatcggc ggaggaatca gaagtcagaa tccagaatca gcacggtgca
    35041 cgctcgagcg tcgcgggcgc tccagcgtga cagtccccat tccggattct gaattccggg
    35101 ttccatcctc cgatctcggg cccccggccc ctatgtcgtc ttcgtcacct tcttcaggcc
    35161 gcgcggccgg tccggatcgt agccgcgcat ccggctcaag tggaacgcga ggagctgggc
    35221 cggcacgatg gccgcaatcg gcgacaccca ctccggcatg ccggcgggca gggccaccgg
    35281 cgcggcggcc agctcgagcg acgacggctc gttggagaag acgaccgggc gcgcgccgcg
    35341 ctcgcggatc tcgcgaagca ggccttccac ctcgctgctg acggcccccg tcacgttgaa
    35401 caccatcgcc gcgaagccgg gctcgacgag cgcgatcggg ccgtgctgga agtcggccga
    35461 ggagtacggc tcgacggcga cgtacgccag ttccttggcc ttgagggcgg cctcgaacgc
    35521 cgtcgagaag ttgaagccgc ggccgagcac gacgccgtgg cccgcgtcct tcagggccgc
    35581 cgcggcggcc accaccgact cgcgctcgac gtagagcgcc gcggccacgt gctcgggcat
    35641 cgcggccagt tcgcggctcc accgcgcatt ctccgcgagc gccgtcgaga gcatcgccag
    35701 cgccgccagc tgcgccgtgt acgtcttcgt ggcggcgatg ctccgctcct gccccgcgcc
    35761 gagcacgatc gtgtggtccg ccatggccgc cagcggcgag cccgccacgt tcgtcacggc
    35821 cagcgtgagg cagccctgcc gcttcccctc ctcgacgacg ctgatgaggt ccggcgactc
    35881 gccggactgc gagatgccga cgacgagcgc cccggccatc gccggcggcg tgcggtagcg
    35941 cgtgaacagc gacggcgcgg cgagggtcac cgacaggcgg ttgtgcgacc caaacaggta
    36001 ctgcccgtac cgcgcggcgt tgtcggacga cccgcgcgcc gcgatgacga cgtggtgcag
    36061 ccggcgcccg cggatgtccg ccgcgatgcg ctccacggcc ggcgcggcgg ccttcagcag
    36121 cccctcgatg atggcgggct gctcggcgat ctcggaaagc agaacgg
//
LOCUS       DAOSIF010000002        30919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_1666_length_30919_cov_5.099890, whole genome shotgun sequence.
ACCESSION   DAOSIF010000002 DAOSIF010000000
VERSION     DAOSIF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 30919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30919
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_30919_cov_5.099890"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLT35_00135"
     CDS             complement(<1..195)
                     /locus_tag="PLT35_00135"
                     /inference="COORDINATES: protein motif:HMM:NF027503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOG27658.1"
                     /translation="MPQDVYPVKPHIAERAYIRTMEEYQRLYRLSLDNPEWFWGEQAK
                     AVTWFHPWQTVLDSDYEEVDF"
     gene            complement(393..2186)
                     /locus_tag="PLT35_00140"
     CDS             complement(393..2186)
                     /locus_tag="PLT35_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG27659.1"
                     /translation="MGGPRFRIPALLLAGVALARGGAAMPGGAAARQQPTFASGVAVV
                     PIDVRAVDSKTGKAVTDLTADDFTLLEDGVVQPVRLFEMRTLAPDTAQGPATGGRLLI
                     RRTPFDAGSQNHRVFLFVLGRGRLQEPSGGVDALIRFVRQRLLPQDRVAVFAYDRATD
                     FTTDHESIARLLERFKRVHYGIDMDVRLQVESGLSALYGSRKLPKKVQDKIDSLFAGG
                     GAPASRRAAGVQAPAVARLEQDAAKQAERLQLADADRVGREMAALEGDTGGGLASWTS
                     LDEVTNEAFAGISFDDYMQYTAQMLLDLSNCYAGIEYLRHLAGEKHLVFVTERGMYLP
                     RAEDDAALARAASDARVAISTFQTGGLIGQQGGVPQSNWNETFAFKALRTMAELTGGV
                     SSIAESGETAMSRLDEATRVSYLLGYYPSLARMDGSYREIEVRVRRPGVTALYRRGYF
                     AERAIGPFNRRDFITRDRIQAAAGFSREIHDIRIRLEADLVRQKGGAHELAINASIDP
                     SRLAFSTVAGERVGAIDILIVGADADGRVLAQHYQRAALRFNDEGWANVTKNGVPYRA
                     HVEINPGVRQVRFIVYDYRADLVGRADRRVF"
     gene            2299..4401
                     /locus_tag="PLT35_00145"
     CDS             2299..4401
                     /locus_tag="PLT35_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG27660.1"
                     /translation="MDATRRRFSWALGGVMLLALVVRLAYLSQLGGSPLLSILMGDAR
                     QYDEWARRIAGGDWLGTGVFYQAPLYPYFLGSLYTVTGPDPGAVRTVQCALGALACGM
                     LGLAGRTLFGSAAGLAAALLLALYPPAIFFDGLIQKSSLDTLLVASILLLVAALQGGP
                     GWKRLAGLGAAVSLLALNRENARAIHPVIAAWLLLGFRHASRTRRAAWVGVFAAASLA
                     VLLPVGLRNYCVGGAFLVSSSQFGPNFYIGNHAGASGSYEPLVPGRGDAAYERADATR
                     LASEAAGRPLAPSAVSDYWAGKALSFIREQPGQWLALVARKAALAINAAEIPDTESIE
                     AYASASWLLRGLLWLNFGVVLPLGAFGCWASRADWRRLSVLYALLATLLLSVVAFYVV
                     ARYRHPVAPLVLIFAGAGLAAAPEALASGRCAAHSRERTARGRTEATGRVFLRPPAWA
                     RRWGPGVAMAALTAAASNVPMKVTHDETWINLGSLLVQSHRAAEALPVLEKAAAADPS
                     YAVPHYQLGLAHRQLGQPRQALDALSAAIRLKPDYAEAYSALGATLRALGRQDAAVAS
                     FRASARHAPESAEAHTNLGLSLMESGQIEEAVSEHRRAVALAPAAPAPRNNLALALQH
                     AGRLQEAIAEYRAALLLKPDYAEAHANLGMALAAAGDAVGARGHVREALRLARASNQA
                     ELAERIEEALRQLEAPSR"
     gene            complement(4498..8415)
                     /locus_tag="PLT35_00150"
     CDS             complement(4498..8415)
                     /locus_tag="PLT35_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG27661.1"
                     /translation="MTRVRGRFCSILFLAGLLMALGAPAYAQGGSSTLVGTVVDSSGA
                     FIPGADVVAKNNATATEYRTVTDSAGRYAIPAIGAGTYTVTVSLAGFKTVILPDIEVI
                     ANTPASVKAVVLELGSLQESVTVTGATELVQTQTASVTTTLTTQQISRAPLTTRNTLD
                     FVTMLPGVNQTQAPRYSTIMGLPGGALNITIDGLNVQDQALKSSTSSAFFAYINPRLD
                     AVEEVTVSTANPGAESSGQGAVQVRFQTRSGTNTFQGSVYHYMRRTQWNTNYWFNQNQ
                     NYPSLPNDKARIDTFGGRVGGPILKDRLFFFFNYEEFRQPQSVSRTRTVLTPESLAGK
                     MVYVGGPAGGVDLFALAAANGQTSTYDPSMKKILEDINATLSGQSITAGSNSVTNTLT
                     FQTESTGRRIYPTGRIDLNLTKKNRIGVSGYLQRFISKPDMLNSRDPAYPGYKMQGNQ
                     TSWRPSVMFNWRSVLGANLVNEARVGYMGWKGTHFSDNITPGDFPGGRHIVMPLINRP
                     YMIGGANNYEIRSSPALNIENTLNWIKGRHSLSFGGAFTQVGYNDYFKYYVPYVALGF
                     DTTNDPAAGMFNTANFPGASSADLSNARNLYALLTGRVTSLNNTAFLNPDTGKYEYMG
                     PWQYWVKQKEFGFYAQDSWKITSNLTINYGLRYQLQLPFTSANKFFTQLSDYNMLYGI
                     SGATGGKPNYFYSGTPGTGSGVLTVKELKAGEAPFDTDWNNLAPSVGFAWRVPITPGS
                     AWSKILSDDPVVRAGYSKSYTREGLSATSNIFAANPGGSIQVFRNQTLGNLIPAGQSW
                     PLLYRETDRLGAPPFPESPSYPLNVAFSNSINAFAADTPTPWVHSFNVGFQRAITKSM
                     AMEIRYVGTRARGGWVDGGRNINELNILENGFIDEFKRAQANRQINAQTYGWGTAAGN
                     SFKYNGLPGQSPLPIYQAFFSGKPASTAGNPAEYTAPAYGNTTYLGYHTPTNPLPGTM
                     AALFFSDATYRANGIAAGYPANFFYMNPGMSGGAWVTGREQDALQSNYDAVQIELRRR
                     MSSGLLAQASYQYIVRQQSTQNITVREDPMWVDLERGRSEGSSRHAFKINWVWELPFG
                     QGKRFASGVSRGLNLIIGGWTFDGTGRIQSGPILDFGNVRLVGMSDADLKKLFKLRIV
                     PDRTNPRIKRVFMLPDDVIDNTILAFSTSYNTASGYSGAAPQGRYFAPIGSGGCLQPF
                     AGACGENGRVTGRLPNHHYVTGPMFMRWDMSLAKRIELTRKINAELRLEALNVFDRPN
                     FFGVTTTGGTAWTSYQVTGAYRDFNNTQDPGGRLVQVSWRLNF"
     gene            complement(8837..10993)
                     /locus_tag="PLT35_00155"
     CDS             complement(8837..10993)
                     /locus_tag="PLT35_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27662.1"
                     /translation="MRRRNAPGRHPVRGRAAAAAGRVRRAPAPPAAPPASGWRRCAVV
                     FGAVVLVAAGAWAYSTSFAGVLVFDDVISITSNPHVRSLWPLSQAASAPRDTGLAGRP
                     VAALSFAINYALAPSDVRDALAAPGPASSPEERARFLRNIRGYHALNLAVHLAAGLLL
                     FGVARRSFAAPALRARVGESATLLAFAVALVWIVHPLATGAVTYIVQRVESMMGLFYL
                     ATLYCAIRAAGPGPLRAVWAAASVAACALGMGSKEAMVTAPVMVALWDLLIGAGRTTA
                     GRVEPAADVLRRRRALYAGLAATWALLAALVAGSPRSGSVGFGLGGWTWWSYLQTQAG
                     VVAHYLRLAAVPAPLVFDYEWAKAPSFSAVAPQALVLAALLAATVTAGVRRHPAGFVG
                     AWFFLILAPSSSVLPIPTEVAGEHRMYLPLAAVIALAAVAARAAAGRAFGAGASLRGR
                     LTRVQHAPGGARPPGRISAADASTLAAALAVALLFGTMTRARNLDYHSTEALMGDTVA
                     KRPLNLRARVAYGAELLASGRFADAERALAAAAELRGSDMARAQANLHLGSALCAQGK
                     LAEGIARLEHALALDAGLAEAHALLGEAHAARGDVERAGAHFALAAERLPDNPAVLRR
                     VAWQLATARDDRFRDGVKAVELAERAARLTARRDPLALEALMAAYAEQGRFAEAAAAG
                     REAVAVARAQGHDALAKGFQLELAMCEAGQRLREHR"
     gene            complement(11009..11761)
                     /locus_tag="PLT35_00160"
     CDS             complement(11009..11761)
                     /locus_tag="PLT35_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG27663.1"
                     /translation="MINGRKLVVVMPAYNAARTLEKTWREVVGQDVVDLVIVVDDASH
                     DATLDLARGLAKTVVHAHESNRGYGANQKTCYRLALDHGADTVIMVHPDYQYTPKLIP
                     AMAHLVGSGLYDCVLGSRILGGAALASGMPAWRYVANRALTLVGNLLLGTKVSEFHTG
                     YRAFSRRLLEQLPLDANSDDFVFDNEVLAETAWLGYAIGEVSCPTSYAADASSITFWR
                     SVRYGFGCLATACVFRLAKMGLVNSPRFPRKA"
     gene            complement(11758..13245)
                     /locus_tag="PLT35_00165"
     CDS             complement(11758..13245)
                     /locus_tag="PLT35_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27664.1"
                     /translation="MTCRRPQNGLQAGASSRRARATAIAIALALAALVLAIYAPVRHF
                     DFVQLDDPAYITENPHVLRGLTLDGVRWAFTTGHAANWHPLTWLSHMLDAELFGIAPG
                     PHHLTSVLLHALNTLLLFGALLRMTGAVWRSAVVAALFGVHPLRAESVAWVAERKDVL
                     SALFWLLTLRAYAGFAAPAEPAAPRRRRRYALALALFALGLMAKPMLVTLPFTLLLLD
                     WWPLGRLGRTGAWPLVREKIPFVALAAASSAVTLIVQERGGAVSAVSSEPFALRLENA
                     VVSLVAYLRDMLWPAGLTVIYPFPESVPAWQASGACLVLGGISLAAWRLARSHPYVPV
                     GWLWYLGTLLPVAGLVRVGLQARADRYTYVPLIGIFIAVVWGGAELAARAGVRFPRRP
                     ALPAVAPALLAAASILALAAAARHQVGYWKDNVTLWTRATVLTLRVDEFEAHMSLGAA
                     LAAQGRSGEALRHFAEAVRLRPDSAEARRAEYDLRRQTGGAGVPR"
     gene            complement(13262..15310)
                     /locus_tag="PLT35_00170"
     CDS             complement(13262..15310)
                     /locus_tag="PLT35_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG27665.1"
                     /translation="MPRFTFAMNTAPTSPSRGRCTTLWLALAIAAVALAVYAPVRHHG
                     FVSIDDPIYVGENPVVARGLSWDGARWAFTTGHAANWHPVTWLSHMADVRLFGLNAGG
                     HHAVSVLLHAANAILLFLLLKRLTGRAGSSACVAVLFAAHPLHVESVAWVAERKDVLS
                     TLFWLLTAHAYVSYVSCPRSAPRRRGAAYALMLALYALGLMAKPMLVTLPLVLLVLDW
                     WPLGRLRRAGTGEAPVLDETGRARRARAEAWLLAAEKLPLFALAAASSAITIVVQHRG
                     GAVSGLAAVPFASRAGNAVVSAGRYLWKAAWPADLSVFYPYPAALPAVPLVLSALFLA
                     AVTGLAIGAARRRPYLLAGWLWYLVTLAPVSGLVQIGRQAMADRYTYVPLVGIFVMLA
                     WGARDLACSGLLARQAWLRSATRIGVPVVAVALVAALAAAARVQTGYWASSAALWQHA
                     LDVDPQNYFAHNSLGALASDAGRTGEALEHFSRAVRSGPDYPEAFNNLGLMYARLGRT
                     AEAVEQYRKAIALNPRLAAAHGNLGVALVTLGRPDEAIAAYHAAIAIDPAAARYRSNL
                     GAALYGQGRPADAIAHFSAALERDPALAQAHTGLALSLAATDRLEEAARHFTEAARLQ
                     PGSDSAQQYLGTALAALGRFDEATAHLQEALRINPGNDAARHALDMVAARTPPPKAAR
                     "
     gene            complement(15458..16180)
                     /locus_tag="PLT35_00175"
     CDS             complement(15458..16180)
                     /locus_tag="PLT35_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOG27666.1"
                     /translation="MNCPACGSAMDAPTFERLYGRRLTLDVCRQCQGIWFDAQELLQM
                     TPGATLQLLAELAVDRGGARQPWTGAPRCPRCARPLVETHDVQRNTRFTYARCPEGHG
                     RFLTFYQFLRAKNLVRPPSDDEMRRLRAHIRQINCANCGAPVDVERNAACAFCHAPLA
                     IVDPEEIRKTIAELAAAAEEKKGVDPALPVSLAMEQLRAARAFAERAGSRRRPSARDL
                     LFGDSSDSVAGFVRLLSRLGKT"
     gene            16292..17032
                     /locus_tag="PLT35_00180"
     CDS             16292..17032
                     /locus_tag="PLT35_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG27667.1"
                     /translation="MTPSRIAIAMAVVAAILVGASCSAKAQGISQQQAEEMLKELRAI
                     RQALERISAPPSDQAADQRVRLKDAGGLALGRPDAPLTLVEFTDLQCQFCNRFQTTAY
                     QQIKSEYIDSGKVRFVTRDFPLDFHPYAMQAARASRCAGEQGRFWEMRQALTLGYNRL
                     GPAFVTATAQSLALDMTAFDACAASTRHDAAIAKDIEDARSINIEGTPSFVLGRTAPD
                     GLDGVLIVGALPFSAFDAKIKAMLAETR"
     gene            complement(17180..18961)
                     /locus_tag="PLT35_00185"
     CDS             complement(17180..18961)
                     /locus_tag="PLT35_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27668.1"
                     /translation="MAPNRLPARQRLAALLAALLAALLAALPAAPLAQQAAAPPVTVE
                     FRAVTDDGVPLRDLAPEHVTLKVDGRERRLLSLTLVEAGAGGREAPQPGVPAPFATNV
                     PAGSGRDTIVIVDDESVPAGAETGLQDAARQYLASLAGDEAAGVVTIPRGGVNLGPTT
                     DRAAFRAAIAKVTGWAKREESDVDAACRTRTILGSLSNIFRDAAGGPPLTVLFFSGGL
                     TPPMTDGVTRMSTPAGLCEVRARDYRDVEVDLTASPVDMFVVHVPDPATPGAAASFGP
                     VTGLEHLAGSTGNRMIRLVGDHADAMSRLAREHSSRYRATFAPEQSERDGQAHRVSVE
                     TVRPGVRVTARPGVLIPAGGKRAAGKAPSPRDMLRVARVFRGLPLRAAAFASRDESAD
                     RVRVAVMFEPDGQASPLAGAAIGFFDDRGKLVVQATAEPSDLARVPAIVGTVAKRGAY
                     RMRVAATDTSGAAGTVDTEVRLDLAGADAVSLSSLVLGVLEEGRFAGKLQFSGEASAV
                     AYIEIYGRAAGALSADLELAATADAPAGVRGPMTLSRDDSGSRHVAMASIPIGALPPG
                     DLVVRVLVSVDGEPVGRAIRTLRKVLQ"
     gene            19071..20402
                     /locus_tag="PLT35_00190"
     CDS             19071..20402
                     /locus_tag="PLT35_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27669.1"
                     /translation="MLRTALAAGLLLHLAAASAGAQGPAEAPSAVHAVTARAIRASEA
                     GALRVFLDCQQYTPCYWNYVKTELTYVDYVNVREAADVHILVTSLGTGAGGREFTMRF
                     IGLGDFAGMNDELLYVTLPTDTEELARRGFVRMLKLGLVRYQLRAGLAGPLEVTHEAS
                     PSAARPANAARSGSDPWDYWVFRARFNGRLDSESASRDRETGGSFSANRVTEAWKLSL
                     SGYQNYREARYTYSDGSSYTSVRRSFDVTGQAVKSLTPHWSAGAKAFVAQSTYENKDR
                     VLNGTAAVEFNVYPYAESTRRQLTFQYLVAVTDLDYKEETVYQKLRETVASHTLRSSF
                     DLRQTWGSLSADADFSQYFHNPSLRRRSLAVEADVRLFKGFALSVFARTAAIHNQIYL
                     PAGDATDEEVLARQRQLATSFRHTFHVGVSYTFGSIFSNVVNTRLSNFVGG"
     gene            complement(20450..23761)
                     /locus_tag="PLT35_00195"
     CDS             complement(20450..23761)
                     /locus_tag="PLT35_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG27670.1"
                     /translation="MSRTAATRLACVACIILSLPLAAQQRKPQLGYYRQPAIHGDTIV
                     FVAEGDLWRVGVAGGVAQRLTTHPEEESRPAISPDGRVVAYAATYEGPGEVYTLPLEG
                     GVPERMTWDAARPSWLSWTPSGEILFATRRHSTLPNTQLVRLDPATGARAIVPLAQAS
                     DGAFDGAGRALFFTRLPFQGSHTRRYKGGTAQNLWRFDGPGQEAVPLTADYAGTSKTP
                     MAWKGRVYFASDRDGVMNLWSMNERGEDLKQHTTHRGYDVQSPSLSNGRIAYQHGADI
                     RVLDVASGIDRIVPITLVSDFDQLRERWIKNTGDWITSAHLSPNGDRVAITARGQVFV
                     VPALQGRIVEAARNKLVRHRNARFMPDGKSVLTLSDESGEVEFWQVPASGVGPSTQLS
                     RDATVLRWDGLPSPDGRFIAHHDKDQQLWIFDVAKKTQTKVAESAHGRFDDLEWSPDS
                     MWLAYTAPAANQLTQIHLYEIASGRSVPVTSDRYDSSSPVFSPDGKWIYFLSDRNFES
                     LVGSPWGSRQPEPYWDRPTRIYHVALKRGERSPFQPDDELHAPKAADKAEQTEQPDAG
                     AAQAGKAGGAKPAAAKPAVQPVVVDTDGLQTRLLEVPVPAGNYSSLSMDAKRLYFVSR
                     PTGRDGKAALKTLALDNKKAEPETFIEEPQSYELSADGRKILIRKGGAYYVVDAGAKA
                     PSDLSKSAVPLKDWTFSLDPRLEWRQMFAEAWRLERDYFYDRGMHGLDWPAMRQKYEP
                     LVDRVTDRAELSDVLAQMVSELSALHIFVRGGDLRDAEDGAWPASLGAELVRDEKAGG
                     FRVVRVIATDPDRPDRRAPLARPGVEVVEGDLIERVNGTSALTVRDLSALLRGQAGKQ
                     VLLRVKPKAGGPARDVVVEPISAQAEDGLRYDEWEYTRRLAVERAGKGEIGYVHLRAM
                     GGANMAEWMREFYPVFNRSGLIVDVRHNSGGNIDSWILGRLLRKAWFYWQPRVGHPTW
                     NMQFAFRGHMVVLVDQNTASDGEAFAEGFRRLGLGTVMGTRTWGGEIWLSSSNFLVDR
                     GIATAAETGVYGPEGQWLIEGHGVDPDIVVDNLPHETFGGADAQLDAAVRHLQELAAS
                     RPIPVPPPPPYPIKR"
     gene            23845..24354
                     /locus_tag="PLT35_00200"
     CDS             23845..24354
                     /locus_tag="PLT35_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG27671.1"
                     /translation="MLTRSAIRRCPSLRAVAAICAFIVTAAPAPAQGPAAAIKAGRAL
                     EEAGKLDQAITEYGKAIAASPASAEGWLRRGVARNAAGQPGPALQDLDRAIQFDPSSA
                     EAHMGRAVALNGLQRFSEAVSAATRALELRPGYGAAYQARGFAKFRLSDYRGAVEDES
                     QAIESNRRD"
     gene            24460..25539
                     /locus_tag="PLT35_00205"
     CDS             24460..25539
                     /locus_tag="PLT35_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27672.1"
                     /translation="MTHYFRGRARHALGDLKGALEDANRTIELNPSYPVGYLVRGDYR
                     LALGDRAGAEADYRKCVSLDPQGKLGLSSLAKLTAGAQGGTAAPAAASATAPPAAPAM
                     PAARAAPAAPAAAIRSALPPLDPSEVPPLSSYSNEVKLLAAFRASPTPETRAALARVR
                     VEHALRLARAAEKQRDEKTFRTALGYAESAAELAPDNPAAWLLLGRLYAAIENSPVAA
                     DLAEQAFRAALEVRPGLADAQLGLGQICLRKGSLDRALNELEPAVAADPALATPEVVS
                     TMGWAYIYDAQLDRGIRFFRGLLARQPGIEPVSIGLAVLLHENGSRAEAERTLTALAT
                     NAAAAADARGYAKALLEDWAKGGAR"
     gene            25536..27956
                     /locus_tag="PLT35_00210"
     CDS             25536..27956
                     /locus_tag="PLT35_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27673.1"
                     /translation="MTRRISCLPLSVAAAVVLMCAGAAHRNTAAQAVIQADISELPRR
                     VAELEAAKAAERAKLQASRSGTQEAYRAIDDKYLGEARKLHQSVREPQLRALERASGV
                     QRGTLSQGAAGTAPELGRGAFGDVDTGSLGARQFEAVRQAALKAGYTVQGEGDYFTIK
                     ELGTTVHREPTLYASAPGSSARQAEISRGLGHETSYNVGSARADANVAALKNLEKGGH
                     TLDRPAARLMESPADLQELGKMTQRNMEAGGVRNQALEAQVDMLKTGYSPEAAGIVRP
                     NATAAEKAATLGEFQQQCRDVNAQSVRNTQARSAATMEGLQAAARAAEAKLQQARASG
                     NAEAISSARAEVQAARTEAIEFQAQQRAAREALVRNNPEGARLMAEAQGMKADLVNGP
                     GGPRYRTPQGTKTPSQLVDALTEPPLPGGQLKASWVSRGLNGVGVTLMGFGIYQGVKD
                     AQEQAGREAGERGDGALTSAAKLGAYSVWNSLGFAWAVQSGREGQARSMQQYQDDLKA
                     GRVDSMSFTSFIAARARGVGDGLYQFLGFKGIETAYAEYVNSRAEQALAAANEKAAAT
                     ALARDQQRNLERAAARAASEKAAAEKAAAEQAAARNAAQESAARAGSGRSSGETAAAA
                     KRPAPKPASPPQPKSDDASVMPNLVRTSWSGQITMQPEEGPAVVLPLTVAIDSYNKIS
                     GSLRWSAWFDEQENQRSIDLSGSYDSRTGALTMQADRVFTEESTITQVLSVPTGSGMG
                     TEQKRVEVKETRRAGVKVRAAGAIDGPDAARGNLEISLTNEILVDGASQGADTNVLRG
                     TWRLNRRR"
     gene            28323..29705
                     /locus_tag="PLT35_00215"
     CDS             28323..29705
                     /locus_tag="PLT35_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG27674.1"
                     /translation="MRVLFVVRGFPPDSTGGTEIHAAGLARILWRRGHDITVLAREAR
                     TDLAEHHVRRDHYGQVPVVRVNNNLRETTLFEETYRNPAVDAVARALMDDEPPDVVHV
                     HHLTSLSTGIVEECARRDIPVIVTLHDYWLMCHRGQLLDLDLERCRGPESDRCAVCAG
                     LADSARPAVHRAARWLRVLDDHVPRAIADARRRLASGLARRAVPESAAAEAERRISHM
                     RSVCVGAAHLLAPSKALLEQFVAFGVPRERLQLVELGIDTRPFARLKRQPSERLRIGF
                     VGSLLPSKAPHLAIEAVQGLPPRRAELTVAGSLGPYHGDASYASALRPLLRSPGVRWL
                     NGVAHHKVPSVYESLDVVVVPSIWLENSLLVIREAFAAGLPVVASNLGAMAETVADGR
                     NGLLFEPGSSADLRRVLQRLLDEPGLLDRLREGIPRVRSIDEDAAWTSALYQELWYRR
                     HGRPTPPPEP"
     gene            29875..>30919
                     /locus_tag="PLT35_00220"
     CDS             29875..>30919
                     /locus_tag="PLT35_00220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOG27675.1"
                     /translation="MSNRAHSTDFIRFFSSAQLLRAGESLYTPPRIEAIDQARSTHGA
                     ASQTLHPNLNTPVLALLVVPLTFAGPARGYAIWTALSLVAGLCACALVWRGLRPPGQG
                     ASGLLWLWLAFLVYFPTYASFVLGQVTAGLFLLLAGAWLAARNGRDRLAGVLLGAAVS
                     VKLFAALLLVCFVLQGRWRVAAWSIGAGLCAALATLPFAGLAAWREYFGVIRTVTWFG
                     NSWNASYASFITRILGGTENAPLVDAPALGRAIVALCSAVTLLWMARLTRLASVEAKG
                     EALMAQAAGFDLGYGLTLAVMLLVSPLGWMYYFPVLLLPGYAVWLLTRGGRMRLLRLG
                     LLAAWGLSAVPSTM"
BASE COUNT         4392 a        10972 c        11148 g         4407 t
ORIGIN      
        1 gaagtccacc tcctcgtagt ccgagtcgag gaccgtctgc caggggtgga accacgtgac
       61 ggccttggcc tgctcgcccc agaaccactc ggggttgtcg agcgacaggc ggtagagccg
      121 ctggtactcc tccatcgtgc ggatgtaggc gcgctcggcg atatgcggct tgacgggata
      181 gacgtcctgg ggcacggccg atcctcctgg tcgcgggctg tcgcggcgtt tgtgcgcgca
      241 gaaggcgaga ctacggcacg gccgcgggcg ccgtcaagga gggcggcggc cggcggagcc
      301 cgggcgaggc cccgggggcc gggcggcggc gcggcgggcg gcgctagcgt ccccggagag
      361 cgcgcccggc ggcgcccgga cgccaggccg cgctagaaca ccctccggtc cgcccggccg
      421 acgaggtcgg cgcggtagtc gtagacgatg aagcggacct ggcggacgcc gggattgatc
      481 tcgacgtgcg cccggtaggg cacgccgttc ttcgtgacgt tcgcccaccc ttcgtcattg
      541 aacctgagcg ccgcgcgctg gtagtgctga gccagcaccc ggccgtcggc gtcggcgccg
      601 acgatcagga tgtcgatcgc gcccacgcgc tcgccggcga cggtcgagaa cgcgagcctt
      661 gacggatcga tcgacgcgtt gatcgccagc tcgtgcgcgc cgcccttctg ccggaccaga
      721 tcggcttcga gcctgatccg gatgtcgtgg atctcgcgcg agaagccggc ggccgcctgg
      781 atccggtcgc gcgtgatgaa gtcgcggcgg ttgaacggcc cgatcgcgcg ctcggcgaag
      841 tagccgcgcc ggtagagcgc cgtcacgccc gggcgcctga ccctgacctc gatctcccgg
      901 taggaaccat ccatccgcgc gagcgaaggg tagtagccga ggaggtagct cacccgcgtc
      961 gcctcgtcca ggcggctcat ggccgtctcg ccgctctcgg caatggaaga cacgcccccg
     1021 gtgagctccg ccatcgtacg gagcgccttg aaggcgaagg tctcgttcca gttgctctgc
     1081 ggcacgccgc cctgctggcc gatgaggccg cccgtctgga acgtgctgat ggcgacgcgc
     1141 gcgtcgctcg ccgcgcgcgc cagcgcggca tcgtcctcgg cgcgcggcaa gtacatgccg
     1201 cgctcggtga cgaagaccag gtgcttctcg ccggccaggt ggcgcaggta ctcgatgccg
     1261 gcgtagcagt tgctcagatc gagcagcatc tgcgccgtgt actgcatgta gtcgtcgaag
     1321 gagatgccgg cgaaggcctc gttcgtgacc tcgtccagcg acgtccagga cgccagcccg
     1381 ccgcccgtgt cgccctcgag cgccgccatc tcgcgcccca ccctgtcggc gtcggcaagc
     1441 tgcagccgct ccgcctgctt cgccgcgtcc tgctcgaggc gcgccaccgc cggcgcctgg
     1501 acgccggccg cccggcgcga cgcgggcgcg cccccgccgg cgaacaggct gtcgatcttg
     1561 tcctggacct tcttcgggag cttcctgctt ccgtacaggg ccgacaggcc gctctccacc
     1621 tgcaggcgga cgtccatgtc gatgccgtag tgcacgcgtt tgaagcgctc gaggaggcgc
     1681 gcgatggact cgtggtcggt cgtgaagtcg gtggcgcggt cgtaggcgaa caccgccacg
     1741 cggtcctgcg gcagcaggcg ctggcggacg aagcggatga gcgcatcgac gccgccagac
     1801 ggctcctgca ggcgcccgcg gccgagcacg aagagaaaga cgcggtggtt ctgcgagccg
     1861 gcgtcgaagg gcgtccggcg gatgagcagc ctgccgccgg tcgcgggccc ctgcgccgtg
     1921 tccggcgcga gcgttcgcat ctcgaacagc cggaccggct gcaccacgcc gtcctcgagc
     1981 agcgtgaagt cgtctgcggt cagatccgtg acggccttgc cggtcttgct gtcgacggcg
     2041 cggacgtcga tcggcacgac ggcgacgccg ctcgcgaagg tcggctgctg ccgggcggcc
     2101 gcgccgcccg gcatcgcggc gccgccccgg gccagcgcca ccccggcaag cagcagcgcc
     2161 ggaatcctga accgcggtcc acccatcggg gccctccggc tcccgtcaat actacccgac
     2221 ggcgggcccg gctcggcccc gaacaggcgc agccggttgg cgggaggatg gtttggtggc
     2281 acactgcggc cgacggacat ggacgccacc cggcggcgtt tctcgtgggc tctcggcggc
     2341 gtgatgctgc tcgccctcgt cgtccgcctt gcgtacctgt cgcagctcgg cggcagcccg
     2401 ctgctgtcca tcctgatggg cgacgcccgt cagtacgacg agtgggcccg gcgcatcgca
     2461 ggcggcgact ggctcggcac cggcgtgttc taccaggcgc ccctctatcc ctacttcctc
     2521 ggcagcctgt acaccgtcac ggggcccgat cccggcgcgg ttcgcaccgt ccagtgcgcc
     2581 ctcggcgcgc tggcctgcgg gatgctgggg ctcgccggac ggactctctt cggctccgcc
     2641 gccgggctcg ccgcagcgct gctgctcgcc ctctatccgc ccgcgatctt tttcgacggc
     2701 ttgatccaga agtcgtcgct cgacaccctg ctcgtcgcgt cgatcctgct gctcgtggcc
     2761 gccctccagg gcgggccagg ctggaagcgc ctcgccggcc tgggtgcggc cgtgtcgctg
     2821 ctcgccctca accgcgagaa cgccagggcg atccatcctg tcatcgcggc ctggctcctg
     2881 ctcgggtttc ggcacgcctc ccgcacgcgc cgagcggcct gggtgggcgt gttcgccgct
     2941 gcgtcgctgg ccgtcctcct gcctgtcggc ctgcgcaact actgcgtcgg cggggcgttc
     3001 ctcgtgtcgt cctcgcagtt cggcccgaac ttctacatcg gcaatcacgc cggcgcctcc
     3061 ggcagctacg aaccgctcgt gccgggccgc ggagacgccg cctacgaacg cgcggacgcg
     3121 acgcgcctgg cctcggaagc cgcggggagg cctcttgcgc catcggcagt gtcggactac
     3181 tgggcgggaa aggcgctctc gttcatccgc gaacagcccg ggcagtggct ggcgctcgtg
     3241 gccaggaagg ccgccttggc catcaacgcc gccgagattc cggacaccga gtcgatcgag
     3301 gcgtatgcga gcgcgtcgtg gctgctgcgc ggcctcttgt ggctgaactt cggcgtggtc
     3361 ctgccgctcg gggcgttcgg ctgctgggcc agccgcgccg actggcgccg gctgtccgtc
     3421 ctgtacgcgc tcctggcaac gctcctgctc tcggtcgtcg ccttctacgt cgtggcccga
     3481 taccgtcacc ccgtcgcgcc gctcgtgctg atcttcgccg gggcgggact ggcggcggcg
     3541 ccggaggcgc tcgcctcggg gcgctgcgcc gcgcactcgc gggagcggac ggcgcggggg
     3601 cgcacggagg ccacagggcg agtcttcctg cggccgccgg cgtgggcccg ccgctggggg
     3661 cccggcgtgg cgatggcggc cctgaccgcg gcggcgtcga acgtcccgat gaaggtgacg
     3721 cacgacgaga cgtggatcaa cctggggtct ctactcgtcc agtcgcatcg cgccgccgag
     3781 gcgctccccg tcctggagaa ggcggcggcc gccgacccgt cgtacgccgt gccgcactac
     3841 cagctcggac tcgcccaccg ccagctcggc cagccgcgcc aggcgctcga cgcgctctcc
     3901 gcggcgatcc gcctgaagcc ggactacgcc gaggcataca gcgccctggg agcgacgctg
     3961 cgcgcgctgg gacggcagga cgccgccgtt gcgagcttcc gcgcgtctgc caggcacgcc
     4021 cccgagtcgg ccgaggcgca cacgaacctg gggctgagcc tgatggagtc cgggcagatc
     4081 gaggaggccg tctccgagca ccgtcgcgcg gtcgctctcg cgcccgcagc ccccgctccc
     4141 cgcaacaacc tggcgctggc gctccagcat gccggacggc tccaggaagc gatcgcggag
     4201 taccgggcgg ccctcctgct gaagccggac tacgccgagg cgcacgccaa cctcgggatg
     4261 gcgctggcgg ccgccggcga cgccgtcggg gcccgcggcc atgtcaggga ggcgctccgc
     4321 ctcgcgcgcg cctcgaacca ggcggagctg gcggagcgca tcgaagaggc cctccggcag
     4381 ctcgaagcgc cctcccgctg agagaagacg gcgccgcacg cagaacgggg cacgcggccg
     4441 ggtgaccgcg tgccccgaac cccgaccggc gcgggccggt ccgatgccgc cgtcagccta
     4501 gaagttcaac ctccacgata cctggacgag ccggccgccc gggtcctggg tgttgttgaa
     4561 gtcgcggtac gccccggtga cctggtagct cgtccaggcc gtgccgccgg tggtggtcac
     4621 accgaagaag ttcggcctgt cgaacacgtt cagcgcctcg aggcgcagct cggcgttgat
     4681 cttccgcgtc agctcgatgc gcttggcgag gctcatgtcc caccgcatga acatcggccc
     4741 ggtcacgtag tggtgattcg gcagccggcc ggtcaccctg ccgttctctc cgcacgcccc
     4801 ggcgaacggc tgcaggcagc cgcccgaccc gatcggcgcg aagtagcggc cctgcggcgc
     4861 cgcgccgctg tagccgctcg ccgtgttgta gctggtgctg aaggcgagga tcgtgttgtc
     4921 gatcacgtca tccggcagca tgaacacccg cttgatcctg gggttcgtcc tgtcggggac
     4981 gatccgaagc ttgaacagct tcttcaggtc ggcatcggac atgccgacca gccgcacgtt
     5041 cccgaagtcg aggatcggcc cgctctggat gcggccggtg ccgtcgaacg tccacccgcc
     5101 gatgatcagg ttcaggccgc ggctgacgcc gctggcgaag cgcttgccct gcccgaaggg
     5161 cagctcccac acccagttga tcttgaacgc gtgcctcgac gagccctcgc tccgtccgcg
     5221 ctcgagatcc acccacatcg ggtcctcgcg gacggtgatg ttctgggtgg actgctggcg
     5281 cacgatgtac tggtagctgg cctgggcgag caggccggag ctcatccggc ggcgcagttc
     5341 gatctgcacc gcgtcgtagt tggactggag cgcgtcctgc tcgcgtcccg tcacccacgc
     5401 gcctccgctc atgccggggt tcatgtagaa gaagttcgcc gggtatcccg cggcgatgcc
     5461 gttcgcgcgg tacgtggcgt cgctgaagaa cagggccgcc atcgtgcccg gcagggggtt
     5521 ggtcggggtg tggtagccga ggtacgtcgt gttcccgtac gcgggcgccg tgtactcggc
     5581 cgggttgccc gccgtgctgg cgggcttccc gctgaagaac gcctggtaga tcggcagcgg
     5641 cgactggccg gggaggccgt tgtacttgaa gctgttcccg gccgccgtgc cccagccgta
     5701 ggtctgggcg ttgatctggc ggttggcctg ggcgcgcttg aactcgtcga tgaagccgtt
     5761 ctcgaggatg ttcagctcgt tgatgttccg cccgccgtcc acccagccgc cccgggcgcg
     5821 cgtcccgacg tagcggatct ccatggccat cgacttcgtg atggcgcgct ggaagccgac
     5881 gttgaacgag tgcacccacg gcgtcggcgt gtcggccgcg aaggcgttga tggaattgct
     5941 gaacgccacg ttcagcgggt aggacgggct ctcggggaag ggcggcgccc cgaggcggtc
     6001 ggtctcccgg tagagaagcg gccaggactg gcccgccggg atcaggttgc cgagcgtctg
     6061 gttccggaag acctgtatgc tgcctcccgg gttcgccgcg aagatgttcg acgtcgccga
     6121 gagcccttca cgagtgtagg acttcgagta gccggcgcgg acaaccgggt cgtcgctgag
     6181 gatcttcgac caggcgctgc ccggcgtgat cggcacgcgc cacgcgaagc cgacgctcgg
     6241 cgcgaggttg ttccagtccg tgtcgaacgg cgcctcgccg gccttcagct ccttcaccgt
     6301 gagcacgccg gagcccgtgc cgggcgtgcc gctgtagaag tagttcggct tcccgcccgt
     6361 cgcgccggag atgccgtaca gcatgttgta gtcggacagc tgggtgaaga acttgttggc
     6421 cgacgtgaac ggcagctgca gctggtagcg caggccgtag ttgatcgtga ggttcgaggt
     6481 gatcttccac gagtcctgcg cgtagaaccc gaactccttc tgcttcaccc agtactgcca
     6541 cgggcccatg tactcgtact tgcctgtgtc cgggttcagg aaggcggtgt tgttcagcga
     6601 cgtcacgcgg cccgtcagca gcgcgtacag gttccgcgcg ttgctcaggt ctgcgctgct
     6661 cgcgcccggg aagttcgcgg tgttgaacat ccccgccgcc ggatcgttcg tcgtgtcgaa
     6721 cccgagcgcc acgtacggca cgtagtactt gaagtagtcg ttgtagccga cctgcgtgaa
     6781 cgccccgccg aacgagagcg agtgcctgcc cttgatccag ttcagggtgt tctcgatgtt
     6841 gagcgccggc gagctgcgga tctcgtagtt gttcgcgccg ccgatcatgt acggcctgtt
     6901 gatgagcggc atgacgatgt gcctgccgcc cgggaagtcc ccgggcgtga tgttgtcgga
     6961 gaagtgcgtg cccttccagc ccatgtagcc gacgcgcgcc tcgttgacca ggttcgcgcc
     7021 gagcacggag cgccagttga acatgaccga cggccgccac gacgtctggt tcccctgcat
     7081 cttgtagccg gggtaggccg ggtcgcggct gttcagcatg tcgggcttcg agatgaaccg
     7141 ctgcaggtac cccgacaccc cgatccggtt cttcttcgtc aggttcaggt cgatgcgccc
     7201 ggtcgggtag atgcgccggc ccgtcgactc ggtctggaac gtgagcgtgt tggtcacgga
     7261 gttcgagccc gccgtgatgc tctggccgga cagcgtcgcg ttgatgtcct ccaggatctt
     7321 cttcatcgac ggatcgtagg tcgacgtctg gccgttcgcc gccgccagcg cgaacagatc
     7381 gacgccgccg gccgggccgc ccacgtacac catcttgccc gccagcgact cgggcgtgag
     7441 gaccgtgcgc gtccgcgaca cagactgcgg ctgccggaat tcctcgtagt tgaagaagaa
     7501 gaacagcctg tccttgagga tcgggccgcc gacgcggccg ccgaacgtgt cgatccgcgc
     7561 cttgtcgttc ggcagcgacg gatagttctg gttctggttg aaccagtagt tggtgttcca
     7621 ctgcgtgcgg cgcatgtagt ggtagacgct gccctggaac gtgttcgtgc ccgaccgcgt
     7681 ctggaaccgc acctgcaccg cgccctggcc ggagctctcg gcaccggggt tcgccgtgga
     7741 cacggtgacc tcttcgacgg cgtcgaggcg cgggttgatg taggcgaaga acgcgctgct
     7801 cgtcgacgac ttcagcgcct ggtcctgcac gttgaggccg tcgatggtga tgttcagggc
     7861 tccccccggc aggcccatga tcgtcgagta gcgcggcgcc tgcgtctggt tcacgcccgg
     7921 cagcatcgtc acgaagtcga gggtgttgcg cgtcgtgagc ggcgcccgcg agatctgctg
     7981 cgtcgtcagc gtcgtggtga ccgaggccgt ctgcgtctgc accagctcgg tcgcgccggt
     8041 gacggtcacc gactcctgca ggctgcccag ctcgagcacg acggccttga cgctggccgg
     8101 cgtgttggcg atgacctcga tgtcgggcag gatgaccgtc ttgaagcccg ccagcgagac
     8161 cgtgaccgtg taggtgccgg cgccgatggc gggaatcgcg tagcgcccgg ccgagtccgt
     8221 caccgtccgg tactcggttg ccgtcgcgtt gttcttcgcg acgacgtcgg cgccgggaat
     8281 gaacgcgccc gagctgtcga cgacggtgcc gacaagcgtc gagctgccgc cttgcgcgta
     8341 ggccggcgcg ccgagcgcca tcagcagccc ggcgaggaac aggattgaac agaaccgccc
     8401 tcgcactcgt gtcatggctc ctccctccgc ctcgatcgtg ccgggcaggc cgggcacgat
     8461 ctccctggcc gtcgcacagc ccccgacagg ctgcggcgcg gagacacttc agcccgctcc
     8521 acgggacaga caacccgcga cgattccgcg ctggccaacc acgacgaatc cgcctatggg
     8581 ccgaagctgc gtatgggttc cgatgatgtg ggcgcacggt acgccatccc ggcgccggaa
     8641 gtcaaggcgc gcgaggaggc gtgtcggccg acaagaaggc ggttgcaagc ctcgtaccgg
     8701 cgcaaggggc cgaaggaccg gcggcaccgc cgcaggcgag accggtcggc gccggccgga
     8761 ttcaaccgag ttattccgcc gggcgtccgg ctggataccg ccgccgcgaa gcgccagcca
     8821 tgccggcggc gtccgcctac cggtgctctc ggagccgctg gccggcctcg cacatggcaa
     8881 gctccagctg gaagcccttc gcgagcgcgt cgtgcccctg cgcgcgcgcg acggccacgg
     8941 cttcgcgccc ggccgcggca gcctcggcga agcggccctg ctctgcgtag gcggccatca
     9001 gcgcttccag cgccagcggg tcccgccggg ccgtcaggcg cgcggcgcgc tcggccagct
     9061 cgaccgcttt caccccgtcg cggaagcggt cgtcgcgcgc ggtggccagc tgccacgcca
     9121 cgcggcgcag gacggccggg ttgtccggca gccgctcggc cgccagcgcg aaatgcgccc
     9181 ccgcgcgctc gacgtcgccg cgggcggcgt gggcctcccc caggagcgcg tgcgcctccg
     9241 ccagcccggc gtccagcgcg agcgcgtgct cgaggcgcgc gatgccctcg gccagctttc
     9301 cctgcgcgca cagcgccgat cccaggtgga ggttcgcctg cgcgcgagcc atgtcggagc
     9361 ctctgagttc cgccgcggcg gcaagcgccc gctcggcgtc cgcgaaccgc cccgacgcca
     9421 gcagttccgc tccgtacgcc acgcgggccc ggaggttgag cggccgcttc gcgacggtgt
     9481 cgcccatcaa ggcctcggtc gagtggtagt cgagattgcg ggcgcgggtc atcgtgccga
     9541 acagcagggc caccgccagg gcggcggcca gcgtcgaagc gtcggcggcg gagatccggc
     9601 ccggcggccg cgctcctccc ggcgcgtgct gaacgcgcgt cagacgcccg cgaagggacg
     9661 cgcccgcgcc gaacgcacgc ccggccgcgg cgcgggcagc caccgcggcc agcgcgatca
     9721 ccgcggccag cggcaggtac atccggtgct cgccggcaac ctccgtcggg atgggcagga
     9781 cgctcgacga cggcgcgaga atcaggaaga accacgcgcc gacgaaaccc gccgggtgcc
     9841 gccggactcc ggcggtcacc gtcgcggcca ggagcgccgc cagcaccaga gcctgcggcg
     9901 cgaccgcgct gaacgacggg gccttcgccc actcgtagtc gaacaccagc ggcgccggca
     9961 ccgcggccag ccgcaggtag tgcgcgacga cgccagcctg cgtctgcagg tacgaccacc
    10021 aggtccagcc gccaaggccg aatccgaccg accccgatct cggactgccc gcgaccagcg
    10081 cggccagcag ggcccacgtc gccgccaggc cggcgtagag cgcccggcgc cgtcggagca
    10141 cgtcggccgc cggctcgacg cggcccgcgg tggtccggcc cgcaccgatg agcaggtccc
    10201 acagcgccac catcacgggc gccgtgacca tcgcctcctt gctccccatc ccgagggcgc
    10261 acgcggccac cgacgcggcc gcccacacgg cgcggagcgg cccgggcccg gccgcgcgaa
    10321 tcgcgcagta gagcgtcgcg aggtagaaca gccccatcat cgactcgacg cgctggacga
    10381 tgtaggtgac ggccccggtg gcaagagggt gcacgatcca gacgagcgcc acggcgaagg
    10441 cgaggagcgt cgccgattcg ccgactcgcg cgcgcagcgc cggcgcggcg aacgatcggc
    10501 gggcgacgcc gaacaggagc aggccggccg ccaggtggac ggccaggttc agcgcgtgat
    10561 acccgcggat gttccggagg aaccgcgccc gctcctccgg gctgctcgcg ggccccggag
    10621 cggccagcgc gtcgcggacg tcggatggag cgagggcgta gttgatcgcg aacgagagcg
    10681 ccgccaccgg gcgcccggcg agccccgtgt cgcgcggcgc cgacgcggcc tggctcagcg
    10741 gccagagcga cctgacatgc gggttggacg tgatcgagat cacgtcgtcg aacaccagca
    10801 cgccggcgaa gctcgtcgag taggcccagg cgccggcggc cacgaggacg acggcgccga
    10861 agacgacggc gcaccgacgc cagccgctcg cgggcggcgc cgccggcggc gcgggcgccc
    10921 tgcgaacccg cccggccgcc gccgcggccc gccctcgcac gggatgccgc cccggcgcgt
    10981 tcctgcggcg catcgtcccg cccacgggct acgccttccg cggaaaccgc ggcgaattga
    11041 cgaggcccat cttcgccagc ctgaagacgc acgccgtggc caggcagccg aacccgtagc
    11101 gcacgctccg ccagaacgtg atggacgagg cgtccgccgc gtagctggtc gggcacgaca
    11161 cctcgccaat cgcgtagccg agccaggccg tctccgccag cacctcgttg tcgaacacga
    11221 agtcgtccga gttggcgtcg agcgggagct gttcgagcag cctacgcgag aacgcgcggt
    11281 atccggtgtg gaactccgag accttcgtac cgagcagcag gttgcccacc agcgtcagcg
    11341 cccggttcgc cacgtaccgc cacgccggca tccccgacgc gagcgcggcc ccgccgagga
    11401 tccgggagcc gaggacgcag tcgtacagcc cgctgcccac caggtgcgcc atcgccggga
    11461 tgagcttcgg ggtgtactgg tagtcggggt ggaccatgat gacggtgtcc gcgccgtggt
    11521 cgagggcgag gcggtagcag gtcttctggt tggcgccgta gccgcggttg ctctcgtgcg
    11581 cgtgcacgac cgttttcgcg aggccccgcg ccaggtccag cgtcgcgtcg tggctcgcat
    11641 cgtcaaccac gatgacgagg tccacgacgt cctggccgac gacctcgcgc caggtcttct
    11701 cgagcgtgcg cgccgcgttg taggcgggca tcaccacgac cagcttcctg ccgttgatca
    11761 tcgcggcacc ccggcgccgc ccgtctggcg gcgcaggtcg tactcggctc ggcgcgcctc
    11821 ggccgagtcc gggcgcagcc gcacggcctc ggcgaagtgc cgcagggctt cgcccgagcg
    11881 gccctgtgcg gcgagcgccg cgcccagcga catgtgcgcc tcgaactcgt ccacgcgcag
    11941 cgtgagcacc gtcgcccgcg tccagagggt gacgttgtcc ttccagtatc ccacctgatg
    12001 ccgggccgcg gcggccagag ccaggatcga agccgccgcg agcagggccg gcgccacggc
    12061 gggaagggcc ggccgtcgcg ggaagcggac gccggctcgc gcggccagct cggcgccgcc
    12121 ccagaccacg gcgatgaaga tgccgatgag cggcacgtag gtgtatcggt ctgcccgcgc
    12181 ctgcaggccg acacgcacga ggccggcgac gggcaggagg gtgccgaggt accagagcca
    12241 gccgacgggc acgtacgggt ggcttcgggc cagccgccac gcggccagcg agatgccgcc
    12301 gagcacgagg cacgcgccgg aagcctgcca agcgggaacg gactccggga acgggtagat
    12361 cacggtcagg ccggccggcc acagcatgtc gcgcaggtag gcgacgagcg agacaacggc
    12421 gttctcgagc cggagcgcga agggctccga gctcacggcg ctgacggcgc cgccgcgctc
    12481 ctgcacgatc agcgtcaccg cgctcgacgc cgccgcgagc gcgacgaagg ggatcttctc
    12541 gcggacgagc ggccaggccc ccgtccgccc cagccttcca agcggccacc agtcgaggag
    12601 cagcagcgtg aacgggagcg tcacgagcat cggcttggcc atcaggccga gggcgaacag
    12661 cgcgagcgcc agcgcgtacc ggcgccttcg ccggggcgct gccggctcgg ccggcgccgc
    12721 gaaccccgcg taggcccgca gcgtgagcag ccagaagagc gcgctgagca cgtccttgcg
    12781 ctcggccacc cacgcgaccg actcggcgcg cagcgggtgg acgccaaaga gggccgcgac
    12841 aaccgcgctt cgccagacgg cgccggtcat ccggaggagc gcgccgaaca ggagcagcgt
    12901 gttgagcgcg tggagcagca cgctcgtcag gtggtgcggg ccgggcgcga tgccgaagag
    12961 ctccgcgtcc agcatgtgcg agagccaggt cagcgggtgc cagttcgcgg cgtggcccgt
    13021 cgtgaacgcc cacctgacgc cgtcgagcgt cagccctcgc aggacatgag ggttctcggt
    13081 gatgtaggcc gggtcgtcga gctgcacgaa gtcgaaatgc cgcacggggg cgtagatcgc
    13141 cagcacgagc gcggcgagcg ccagggcgat ggcgatcgcg gtggcgcgcg cgcggcggct
    13201 cgaggcgccg gcctggaggc cgttttgcgg ccttcggcat gtcaccgccg caccccgggg
    13261 atcatcttgc ggccttcggc ggcggcgtcc gcgccgccac catgtcgagc gcgtgccgcg
    13321 cggcgtcgtt gcccgggttg atgcgaagcg cctcctgcag gtgcgccgtc gcctcgtcga
    13381 accggccgag ggcggcaagc gccgtgccga ggtactgctg ggccgagtcc gagcctggct
    13441 gcagccgggc ggcctcggtg aagtgccggg cggcttcttc cagcctgtcg gtggccgcga
    13501 gcgagagcgc gaggcccgtg tgcgcctgcg ccaatgcggg atcgcgctcg agcgcggcgc
    13561 tgaagtgggc gatggcgtcg gccggccgcc cctggccgta cagcgccgca ccgaggttgc
    13621 tccggtagcg cgccgcggcc gggtcgatgg cgatggctgc gtggtaggcc gcgatggcct
    13681 cgtccggccg gcccagcgtc acgagcgcga ccccgaggtt gccgtgcgcg gccgccaggc
    13741 gtgggttcag ggcgatcgcc ttgcggtact gctccaccgc ctcggccgtc cgtcccaggc
    13801 gcgcgtacat cagcccgagg ttgttgaacg cctccgggta gtccggcccg gaccgcacgg
    13861 cgcgcgagaa gtgctccagc gcctcgccgg tccggccggc atcgctggcg agggcgccga
    13921 gggagttgtg cgcgaagtag ttctgcggat cgacatccag cgcgtgctgc caaagggccg
    13981 cgctgcttgc ccagtagcct gtctgcacgc gcgcggcggc ggcgagcgct gcgacaagcg
    14041 ccaccgccac gacgggcacg ccgatgcgag tcgcgctcct cagccaggcc tggcgcgcga
    14101 ggagcccgct gcaggcaaga tcgcgagcgc cccaggccag catcacgaag atgccgacga
    14161 ggggcacgta cgtgtagcgg tcggccatcg cctgccgccc gatctggacg agtccggaaa
    14221 ccggcgcgag cgtgacgagg taccagagcc agccggcgag gaggtacggc cggcggcgcg
    14281 ccgccccgat cgcgagtccg gtcacggccg ccaggaaaag ggccgacaag acgaggggaa
    14341 cggcaggaag cgctgccggg tagggataga agacgctgag atcggccggc cacgcagcct
    14401 tccagaggta gcgtccagcg ctcacgacgg cgttgcccgc gcggctggcg aatggcacgg
    14461 ccgccaggcc gctcaccgcc ccgccgcggt gctggacgac gatcgtgatc gcgctcgacg
    14521 cggcggccag cgcgaaaagc gggagtttct cggccgccag gagccaggcc tccgcgcgag
    14581 cgcggcgcgc gcggccggtc tcatcgagaa cgggcgcttc gcccgtcccc gcccgccgca
    14641 gcctgccgag cggccaccag tcgagaacga gcagcacgag cgggagcgtc accagcatcg
    14701 gcttggccat caggcccagc gcgtagagcg cgagcatgag cgcgtaggcg gcgccccgcc
    14761 ggcgcggcgc cgaccgcggg cacgacacgt acgacacgta ggcgtgggcc gtgagcagcc
    14821 agaacagcgt gctcagcacg tccttgcgct cggcgaccca ggccaccgac tccacgtgca
    14881 gcgggtgcgc cgcaaacagc acggccacgc acgcgctcga gcccgcgcgg ccggtcagcc
    14941 gcttcagcag caggaacagc aggatcgcgt tggccgcgtg caggagcacg ctcaccgcgt
    15001 ggtgcccgcc ggcgttcagg ccgaacagcc ggacgtcggc catgtgcgac agccacgtca
    15061 ccgggtgcca gttggcggcg tggccggtcg tgaacgccca cctggcgccg tcccacgaca
    15121 ggccccgggc cacgaccggg ttctcgccga cgtagatcgg gtcgtcgatc gacacgaagc
    15181 cgtggtggcg gacgggcgcg tacacggcca gcgccacagc ggcgatcgcg agcgccagcc
    15241 acagcgtcgt gcaacgtccc cgggacggcg aagtcggcgc ggtgttcatg gcgaacgtga
    15301 accggggcac gggtgcctgg agcgggagga tagcacggcg acttgggctg ggggctcggg
    15361 attccgggtt cggaaccgca gagcatggtg cccgccttcg cgaatccggg aggcccggca
    15421 atcagacacg cgcgcgggcc ggcgacgggg cctattctca ggtcttcccg aggcgggaca
    15481 ggaggcggac gaagccggcc actgagtcgg acgagtcgcc gaagagcaga tcgcgcgcgg
    15541 acggccggcg gcgcgagccc gcgcgttcgg cgaaggcccg cgcggcgcgc agctgttcca
    15601 tggcgagcga gacgggcagc gccggatcca cgcccttctt ctcctcggcc gccgccgcca
    15661 gctccgcgat ggtcttcctg atctcctccg gatcgacgat ggcgagcggc gcgtggcaga
    15721 aggcgcaggc ggcgttgcgc tcgacgtcga ccggcgcgcc gcagttggcg cagttgatct
    15781 gccggatgtg cgcgcgcaac cgccgcatct cgtcgtcgct gggcggccgg acgaggttct
    15841 tggcgcgcag gaactggtag aaggtgagga accgcccgtg cccctccggg caccgggcgt
    15901 acgtgaagcg cgtgttccgc tggacgtcgt gcgtttcgac gagcgggcgc gcgcagcgcg
    15961 gacaccgggg cgccccggtc cagggctggc gcgccccgcc gcggtccacg gcgagttcgg
    16021 ccagcagctg cagcgtcgcg cccggcgtca tctgcagcag ttcctgggcg tcgaaccaga
    16081 tgccctggca ctgccggcag acatcgaggg tcagccggcg gccgtagagc cgttcgaagg
    16141 tgggggcatc catggccgat ccgcaggcgg ggcagttcac gggcaggtct ccatcgcttc
    16201 ctttcttgtc ggcccgtcgc ggccggaggg ataggagccg tgtgcgggcc cggccgggcg
    16261 gcggcccgca ccctcgggta agatggatgc catgacgcca agccgcatcg ccattgccat
    16321 ggccgtcgtc gccgcgatcc tggtcggcgc ctcctgcagc gccaaggcgc agggcatcag
    16381 ccagcagcag gccgaggaga tgctgaagga gctgcgcgcc atccgccagg ccctcgagcg
    16441 catctcggcg cctccgtcgg accaggctgc ggaccagcgg gtgcggctga aggacgcggg
    16501 cggcctcgcc ctcggccgcc ccgacgcgcc gctgacgctc gtcgagttca cggacctgca
    16561 gtgccagttc tgcaacaggt tccagaccac cgcgtaccag cagatcaagt cggagtacat
    16621 cgactccggg aaggtgcgct tcgtgacgag ggacttcccg ctggacttcc atccgtacgc
    16681 gatgcaggcg gccagggcct cgcggtgcgc cggcgaacaa ggcaggttct gggagatgcg
    16741 gcaggccctg acgctcggct acaaccggct cggccccgcg ttcgtcaccg cgacggcgca
    16801 gtcgctcgcg ctcgacatga cggcgttcga cgcctgcgcg gccagcacgc ggcacgacgc
    16861 cgccattgcg aaggacatcg aggacgcgcg ctcgatcaac atcgagggga cgccctcgtt
    16921 cgtgctgggc cggaccgcgc cggacggcct cgacggcgtc ctgattgtcg gcgccctgcc
    16981 attctcggcg ttcgacgcga agatcaaggc gatgctggcg gagacgcggt aggcgcggat
    17041 gagctgccgg ctctgaaggc ccggcctccg tccttcgcgt tcctcgtcgg ttgcgggaac
    17101 ccggccccgg cgcgccgagg cggccgtcgc gcgagggtgg cacgcggggg cgctccgcgt
    17161 ccgagccgag ggcacgcggc tactgcagga ccttgcgcaa cgtcctgatc gcgcggccga
    17221 cgggctcgcc gtcgacgctc acaagcacgc ggacgaccag atcgcccggc gggagcgcgc
    17281 cgatgggaat gctggccatc gcgacgtgcc ggctgcccga gtcgtcgcgc gagagggtca
    17341 tcgggccgcg cacgccggcc ggcgcatctg ccgtcgccgc cagttcgagg tccgccgaga
    17401 gcgcgcctgc cgcgcggccg tagatctcga tgtaggccac agccgaggcc tcgcccgaga
    17461 actggagctt cccggcgaac cgcccttcct cgagcacgcc gagcaccagc gaactgagcg
    17521 agaccgcgtc cgccccggca aggtcgaggc gaacctcggt gtccacggtg ccggccgcgc
    17581 ccgatgtgtc cgtcgccgcg acgcgcatgc ggtaggcgcc gcgcttggcc accgtgccta
    17641 caatcgccgg cacccgcgcc aggtccgacg gctcggcggt cgcctgcacg acgagcttgc
    17701 cgcgatcgtc gaagaagccg atggcggcgc cggcgagcgg cgaggcctgg ccgtccggct
    17761 cgaacatcac cgccacgcgg acgcggtccg ccgactcgtc cctggacgcg aaggccgccg
    17821 cgcgaagcgg caggccgcgg aagacgcggg cgacgcggag catgtcgcgc ggcgacggcg
    17881 ccttgccggc ggcgcgcttc cccccggccg ggatgagcac gcccgggcgc gccgtcaccc
    17941 tcacgccggg gcgaacggtc tccacgctga cgcggtgcgc ctggccgtcc cgctccgact
    18001 gctccggcgc gaaggtggcg cgataccgcg acgagtgctc gcgggccagg cgcgacatgg
    18061 cgtcggcgtg atcgcctacg agccggatca tgcggttgcc cgtcgagccg gcgagatgct
    18121 cgaggccggt gaccggcccg aacgacgccg ccgctcccgg cgtggccggg tcgggcacgt
    18181 ggacgacgaa catgtcgacg ggcgaggccg tgagatccac ctcgacgtcg cggtagtccc
    18241 tggcccgcac ctcgcagagc cccgccgggg tggacatcct ggtcacgccg tcggtcatcg
    18301 ggggcgtgag gcctcccgag aagaacagca ccgtcagcgg cgggccgccc gccgcgtcgc
    18361 ggaagatgtt gctgagcgat ccgaggatcg tgcgggtccg gcaggcggca tcgacgtccg
    18421 actcctcgcg tttcgcccag ccggtcacct tggcgattgc ggcccggaag gccgcccgat
    18481 cggtcgtggg cccgaggttg acgccgcctc gtgggattgt gaccacgccg gccgcctcat
    18541 cgccggcgag gctcgccagg tactggcgcg cggcgtcctg caggcccgtc tcggcgccgg
    18601 ccggaaccga ctcgtcgtcc acgatgacga ttgtgtcgcg gccgctcccc gccgggacgt
    18661 tcgtcgcaaa cggtgccggc acgccgggct gcggcgcctc gcggccgccg gcgcccgctt
    18721 cgacgagggt cagcgagagg agccgcctct cgcgcccatc aaccttcagc gtgacgtgct
    18781 cgggcgcgag gtcccgaagc ggcacgccgt cgtcggtgac ggcgcggaac tccaccgtaa
    18841 cgggtggagc ggcggcttgc tgggcgagcg gcgcggcggg cagcgcggcc agcagcgcgg
    18901 cgagcagcgc ggccagcagc gcggcgagcc gttgacgcgc cggaaggcgg ttcggggcca
    18961 tcctcagaga gtaaggggtc gggtctcatt ctgcaaacga cgaattctgt aattcaattt
    19021 cgtcgtttgc agaatgagac ccgacccctt acgatctcgt cgaggtgctg atgctgcgaa
    19081 cggctctggc tgcaggactc ctcctccacc tggcggcggc gagcgcaggg gcgcaggggc
    19141 ctgccgaggc cccatcggcc gtccacgccg tcaccgcgcg cgccatccgc gcatccgagg
    19201 ccggcgccct gcgtgtcttc ctcgactgcc agcagtacac gccctgctac tggaactacg
    19261 tcaagacgga gctgacctac gtcgactacg tgaacgtccg ggaggcggcc gacgtgcaca
    19321 tcctggtgac gtcgctcggg accggcgccg gcggccgcga gttcacgatg aggttcatcg
    19381 gcctcggcga cttcgccggc atgaacgacg agctgctcta cgtcacgctg ccgaccgaca
    19441 ccgaggagct cgctcgccgc ggcttcgtgc gcatgctgaa actcggcctg gtgcgctacc
    19501 agctgcgcgc gggtctggcc ggccctctcg aggtcaccca cgaggcgtcc ccgtcggcgg
    19561 cgcggcccgc caacgcggcg cgatcgggat cggatccttg ggactactgg gtcttccgcg
    19621 cgcggttcaa cggccggctc gacagcgagt cggcgagccg ggaccgcgag accggcggat
    19681 ccttttcggc gaaccgggtg accgaagcgt ggaagctgag tctctccggg taccagaact
    19741 acagggaggc ccggtacacg tacagcgacg gctcgtcgta caccagcgtc cgccgcagct
    19801 tcgacgtcac cggccaggcc gtcaagagcc tcacgccgca ctggtcggcc ggcgccaagg
    19861 cgttcgtcgc gcagtccacg tacgagaata aggaccgcgt gctcaacggg accgcggccg
    19921 tcgagttcaa cgtgtatccg tacgcggagt ccacgcggcg tcagctcacg ttccagtacc
    19981 tggtcgccgt gacggacctc gactacaagg aggagacggt ctaccagaag ctccgcgaga
    20041 cggtcgcgag ccacacgctc cgctcgtcgt tcgacctcag gcagacgtgg ggatcgctga
    20101 gcgccgacgc ggacttctcg cagtacttcc acaacccgtc gctgcggcgc cgatcgctcg
    20161 ccgtcgaggc ggacgtccgg ctcttcaagg gcttcgcgct cagtgtcttc gccaggaccg
    20221 ccgccattca caaccaaatc tacctgccgg cgggtgacgc caccgacgag gaggttctgg
    20281 cgcgccagcg ccagctcgcg acctccttcc ggcacacgtt ccacgtcggc gtcagctaca
    20341 ccttcggctc catcttcagc aacgtcgtca acacgcgcct gtcgaacttc gtcggcggat
    20401 agtgccgggg tcggatccgc ggatttccgg atctgacccc agctcctgct caccgcttga
    20461 tcgggtacgg cggcggcggc ggcaccggga tcggcctgct cgccgcgagc tcctgcaggt
    20521 gccgcacggc cgcatcgagc tgcgcgtccg ccccgccgaa ggtctcgtgc ggcaggttgt
    20581 ccacgacgat gtcggggtcg acgccgtggc cttcgatgag ccactggcct tcggggccgt
    20641 agacgcccgt ctcggcggcc gtcgcgatgc cgcgatccac gaggaagttg ctcgagctca
    20701 gccagatctc ccctccccac gtccgggtgc ccatcaccgt gcccagcccg agccgcctga
    20761 agccctcggc gaacgcctcg ccgtcggacg cggtgttctg atcgaccagc acgaccatgt
    20821 ggccgcggaa cgcgaactgc atgttccacg tcggatggcc gacgcgcggc tgccagtaga
    20881 accacgcctt ccgcagcagg cgcccgagta tccagctgtc gatgttgccg ccgctgttgt
    20941 ggcgcacgtc cacgatgagc cccgagcggt tgaacacggg gtagaactcg cgcatccact
    21001 cggccatgtt cgcgccgccc atggcccgga ggtggacgta tccgatctcg cccttcccgg
    21061 cgcgctcgac ggcgagccgc cgcgtgtact cccattcgtc gtagcgcagg ccgtcttcgg
    21121 cctgcgcgct gatcggctcg acgacgacgt ctcgcgcggg gccgccggcc ttcggcttca
    21181 cgcgcaggag cacctgcttg ccggcctggc cccgcagcag cgccgacaga tcccggacgg
    21241 tcagcgcgct ggtgccgttc acccgctcga tgaggtcgcc ctcgacgacc tcgacgccgg
    21301 gccgcgcgag cggcgcacgc cgatcggggc gatcggggtc cgtggcgatg acccggacga
    21361 ccctgaagcc gccggccttc tcgtcgcgga cgagctccgc cccgagcgac gccggccacg
    21421 cgccgtcctc ggcgtcgcgc agatcgccgc cgcgcacgaa gatgtggagg gccgagagct
    21481 ccgagaccat ctgcgccagg acgtcgctga gttccgcgcg gtcggtcacc cgatcgacca
    21541 gcggctcgta cttctgccgc atcgcgggcc agtcgaggcc atgcatgccg cggtcgtaga
    21601 agtagtcgcg ctcgaggcgc caggcttcgg cgaacatctg ccgccactcg aggcgcggat
    21661 cgaggctgaa cgtccagtcc ttcagcggca cggccgactt cgagaggtcc gacggcgcct
    21721 tggcgccggc gtccaccacg tagtaggcgc cccccttgcg gatcaggatc ttcctgccgt
    21781 cggccgacag ctcgtagctt tgcggctcct cgatgaacgt ctccggctcg gccttcttgt
    21841 tgtcgagcgc gagcgtcttc agcgccgctt tgccgtcgcg gcccgtcggg cgcgacacga
    21901 agtagagccg cttggcgtcc attgacaggc tgctgtagtt gccagccggc accggcacct
    21961 cgagcagacg cgtctgcagc ccgtcggtat cgacgacgac gggctgcacc gcgggtttgg
    22021 ccgcggccgg cttagcccct ccggccttgc cggcctgcgc ggccccggcg tcgggctgct
    22081 ccgtctgctc ggccttgtcg gcggccttcg gcgcgtgcag ttcatcgtcg ggctggaagg
    22141 gcgagcgctc gccgcgtttc agggcgacat ggtaaatcct cgtcggccgg tcccagtacg
    22201 gctccggctg ccgagagccc cacgggctgc cgacgagcga ctcgaagttc cggtcggaca
    22261 ggaagtagat ccacttcccg tccgggctga agaccgggct gctgctgtcg taccggtccg
    22321 acgtcaccgg gacgctccgt cccgacgcga tctcgtacag gtggatctgc gtgagctggt
    22381 tcgcggccgg agcggtgtac gccagccaca tcgagtcggg cgaccactcg aggtcgtcga
    22441 agcggccgtg ggccgactcg gcgactttcg tctgcgtctt cttcgcgacg tcgaagatcc
    22501 agagctgctg gtccttgtcg tggtgcgcga tgaagcggcc gtccggcgac ggcaggccgt
    22561 cccaccggag caccgtggca tcgcggctca gctgcgtgga cggccccacg ccgcttgccg
    22621 ggacctgcca gaactccacc tcgccgctct cgtccgatag cgtcaggacg ctcttgccgt
    22681 ccggcatgaa gcgggcgttg cggtggcgga cgagcttgtt gcgcgcggcc tccacgatgc
    22741 ggccctgtag cgcggggacc acgaacacct gtccgcgcgc cgtgatggcc acgcggtcgc
    22801 cgttcggaga gaggtgcgcg gacgtgatcc agtccccggt gttcttgatc caccgctcgc
    22861 gaagctgatc gaagtcagac acgagcgtga tgggcacgat cctgtcgatg ccggaggcga
    22921 cgtcgaggac gcggatgtcg gcgccgtgct ggtaggcgat ccggccgttc gagagcgacg
    22981 gcgactgcac gtcgtagccg cggtgcgtcg tgtgctgctt caggtcctcg ccgcgctcgt
    23041 tcatcgacca gaggttcatc acgccgtcgc ggtccgaggc gaagtagacg cgccccttcc
    23101 aggccatcgg ggtcttgctg gtgccggcgt agtccgccgt gagcgggacg gcctcctggc
    23161 cggggccgtc gaaccgccag aggttctgcg cggtgccgcc cttgtagcgc cgcgtgtggc
    23221 tgccctggaa cgggaggcgc gtgaaaaaga gggcgcggcc ggccccgtcg aacgcgccgt
    23281 cggacgcctg ggccagcggc acgatcgcgc gcgcacccgt ggcgggatcg aggcggacaa
    23341 gctgcgtgtt gggcagcgtg gagtgccggc gggtggcgaa gaggatctcg cccgatggcg
    23401 tccacgacag ccaggagggg cgcgccgcgt cccacgtcat gcgctcggga accccgccct
    23461 ccaggggcag cgtgtagact tcgccgggcc cctcgtaggt ggccgcgtac gcgacgaccc
    23521 ttccgtcggg cgagatcgcg ggccggctct cttcctccgg gtgcgtcgtc aggcgctggg
    23581 ccacgccgcc cgccacgccc acgcgccaga ggtcgccctc ggcgacgaag acgatcgtgt
    23641 cgccgtggat cgccggttgg cggtagtagc ccagctgcgg tttccgttgc tgcgcggcga
    23701 ggggcaggct taggatgatg caggcgacgc aggcgagtct cgtggcggct gtgcgcgaca
    23761 tggtcatctc ctgagatgcc gtgggaacgc gtgtgagagc gcattctagc agcccgcgtc
    23821 cgcgctccgc tccggaggtc agcgatgctc actcgctctg cgatcaggcg ctgcccgtcc
    23881 ctccgcgccg tcgccgcaat ctgcgccttc atcgtgaccg cggcgccggc gccagcccag
    23941 ggcccggccg cggccatcaa ggccggccgg gcgctcgagg aggccggcaa gctggaccag
    24001 gccatcacgg agtacggcaa ggccatcgcc gcgagccccg cgtcggccga aggctggctc
    24061 cgccgcggcg ttgccaggaa cgcggcaggt cagcccgggc cggcgctgca ggatctcgac
    24121 cgcgcgattc agttcgatcc ttccagcgcc gaggcccaca tgggccgggc ggtcgcgctc
    24181 aacggcctgc agcggttcag cgaggccgtg tcggccgcca cgcgtgcgct cgagctgcgg
    24241 cccggatacg gggccgccta ccaagcgcgg ggcttcgcga agttcaggct ctccgattac
    24301 cgcggcgccg tggaagacga gtcgcaggct atcgagtcca accggagaga ctagggcgcc
    24361 taccggattc gcgggatcgc ggaagtcaac ctgaacctcc accgtgaggc gctgcgtgac
    24421 ctggacaagg cgatcgacgc cgggatcggc ggcgcggaca tgacgcacta cttccgcggg
    24481 agggcacggc atgcgctcgg cgacctgaag ggcgcgctcg aggacgcgaa ccgcacgatc
    24541 gagctgaacc cgagctatcc tgtcggctac ctcgtgcggg gggactacag gctggccctc
    24601 ggcgaccgcg ccggggccga ggcggactat cggaagtgcg tctcgctcga cccgcaaggg
    24661 aagctggggc tgtcgtcgct cgccaagctg acagccggcg cgcagggcgg aactgctgcg
    24721 ccggcggcag cgtcggcaac ggcgcctcca gccgcaccgg caatgccggc agcccgcgcg
    24781 gcgcccgcgg cgcccgcggc ggcaattcgc tcggcgctgc cgccgctcga tccgtccgag
    24841 gttccgccgc tcagttcgta ctcgaacgag gtgaagctcc tggcggcgtt ccgcgccagc
    24901 ccgacgccgg agacgcgggc cgcgctcgcc cgggtgcgcg tcgaacacgc tctgcgcctc
    24961 gcccgggcag ctgagaagca gcgggacgaa aagacgttca ggacggcgct tggctacgcg
    25021 gagtcggccg ccgagctcgc accggacaac cccgctgcgt ggctcctcct gggccgactg
    25081 tacgcggcca tcgagaacag cccggtcgcc gccgacctgg ccgagcaggc cttccgtgcc
    25141 gcactcgagg tgcgtcccgg actggccgat gcgcagctcg ggctcggtca gatctgcctc
    25201 cggaagggct cgctcgaccg cgccctgaac gagctggagc cggccgtcgc cgcagacccc
    25261 gcgctcgcca cgccggaggt ggtttcgacg atggggtggg cctacatcta cgacgcccag
    25321 ctcgaccgcg gcatcaggtt cttccgcggc ctcctcgcca ggcagcccgg gatcgagccc
    25381 gtgtcaatcg gcctcgcggt cctgctccac gagaacggct cgcgggcaga agccgagcgc
    25441 acgttgaccg cgctcgcgac caacgcggcc gcggcggccg acgcgcgagg ctacgcgaaa
    25501 gcgctcctcg aggattgggc gaagggaggt gcgcgatgac gcgccggatc tcctgcctcc
    25561 cgctgtcagt cgcggccgca gtcgtgctca tgtgcgccgg cgcggctcat cggaataccg
    25621 ccgcgcaggc ggtgatccag gccgacatct cggaactgcc gaggcgcgtg gcggaactgg
    25681 aggccgcgaa ggccgccgag cgcgcgaagc tccaggcctc gcggtcgggg acccaggagg
    25741 cgtaccgggc catcgacgac aagtacctgg gcgaggccag gaagctccac cagagcgtcc
    25801 gcgagccgca actgcgcgcg ctcgagcggg cgtccggcgt gcagcgcggc accctcagcc
    25861 agggcgccgc cggcaccgcg cccgagctgg gccgaggcgc cttcggggac gtcgacaccg
    25921 ggtcgctcgg cgcacgccag ttcgaggcgg tccggcaggc tgccctgaag gcgggctaca
    25981 ccgttcaagg ggagggcgac tatttcacca tcaaggagct gggcaccacc gtgcaccgcg
    26041 agccgacgct gtacgcgtcg gcgcccggca gttcggcgcg acaggccgag atcagccgcg
    26101 gcctcgggca cgagacgtcc tacaacgtgg gcagcgcgag agccgacgcg aacgtcgccg
    26161 cgctgaagaa cctcgagaag ggtgggcaca cgctcgaccg gcccgcggcg cggctcatgg
    26221 agtcgcccgc ggatctccag gaactcggga agatgaccca gcgcaacatg gaggccggcg
    26281 gcgtccgcaa tcaagcgctg gaggcacagg tcgacatgct caagaccggc tactcgcccg
    26341 aggccgcggg gatcgtccgg ccgaacgcca cggcggcgga gaaggccgcc acgctcggcg
    26401 agttccagca gcagtgccgc gacgtgaacg cccagtccgt ccggaacacg caggcgcggt
    26461 ccgccgcgac catggaaggc ctgcaggccg ccgcgcgggc ggccgaggcg aagctgcagc
    26521 aggcccgggc gagcggaaat gcggaggcga tctcgtcggc ccgcgcggag gtgcaggcgg
    26581 cgagaacgga ggccatcgag ttccaggctc agcagcgcgc ggcccgcgaa gccctggtcc
    26641 ggaacaaccc cgaaggcgct cggctcatgg ccgaagcgca gggcatgaag gccgacctcg
    26701 tcaacgggcc cggcggcccg cgctaccgca cgccgcaggg aacgaaaacg ccgagccagc
    26761 tggtggacgc gctgacggag ccgccgcttc ccgggggcca gctcaaggcg agctgggtgt
    26821 cgcgcggcct gaacggggtc ggcgtgacgc tgatgggatt cggcatctac cagggcgtca
    26881 aggacgccca ggagcaggcg ggccgggagg ccggcgagcg cggcgacggc gcgctcacga
    26941 gcgcggcgaa actgggcgcg tacagcgtct ggaacagcct cggtttcgca tgggcggtgc
    27001 agtcgggccg ggagggccag gcgcggtcga tgcagcagta ccaggacgac ctgaaggcgg
    27061 gccgggtcga ttcgatgtcg ttcacgtcgt tcatcgccgc gcgggcgagg ggcgtcggag
    27121 acgggctcta ccagttcctg ggattcaagg gcatcgagac cgcgtacgcg gagtacgtga
    27181 acagccgggc cgagcaggcc ctcgcggcgg ccaacgagaa ggccgcagcc acggcgctgg
    27241 cgcgggacca gcagcgcaac ctggagcggg cggcagcgcg tgccgcgtct gagaaggcgg
    27301 cggcggaaaa ggccgcagcc gaacaagccg cagcccggaa tgccgctcag gaatccgcgg
    27361 cccgcgcggg gtccggccgc agcagcggtg aaaccgcggc cgccgcgaag cgtccggccc
    27421 cgaagccggc ctcgcctccg cagcccaaga gcgacgatgc cagcgtgatg ccgaacctcg
    27481 ttcgcacctc gtggtcgggg cagatcacga tgcagcccga agagggcccg gccgtcgtgc
    27541 ttccgctcac ggtggccatc gacagctaca acaagatcag cggcagtctc cgttggagcg
    27601 catggttcga cgagcaggag aaccagaggt cgatcgacct gagcggctcg tacgactcca
    27661 ggacgggggc gctcacgatg caggccgaca gggtctttac cgaggagtcc accatcaccc
    27721 aggtgttgag cgtgccgacc ggcagcggca tgggcaccga gcagaagcgg gtcgaggtga
    27781 aagagaccag gcgcgcgggc gtcaaggtgc gggctgcagg cgcgatcgac ggccccgatg
    27841 ccgcacgcgg caacctggag atctcgttga cgaacgagat cctcgttgac ggcgccagcc
    27901 aaggcgccga tacgaacgtg ttgcgcggca cctggcgcct gaatcgccgg cgctgaaccg
    27961 ccgccgggtc gtacgaaagg ggtcgggtcg atttaccgcg atcacagaat tggcttacag
    28021 atctgttgag tgcggtaaat cgacccgacc cctttgtgcg ctcgccgagg acgcgatgga
    28081 atgggaggga cgttatgaga gactaatggg cagggcgtcg taacctgcgc gcgccggcgt
    28141 tgcggccact cgagatcagg gtctcgaacc ggccgtctcg atcaacaatg tgcgcgctcc
    28201 tgtttcgtct ggcgcatggg tatcccactc gtctccgtcc tgctcctcat agggaacgtc
    28261 acggcgcatc tgcgcgaccg ggcggttcgt ctggtcgagg ccccgcgcga ggagggcgcc
    28321 tgatgcgcgt cctcttcgtc gtgcgcggct tccctcccga ttcgaccggc gggaccgaga
    28381 tccacgcggc cggcctcgcc cgcatcctct ggcgccgggg ccacgacatc acggtgctgg
    28441 cgcgggaggc caggacggat ctggccgagc accacgtaag gcgcgatcac tacgggcagg
    28501 tcccggtcgt ccgcgtgaac aacaacctcc gcgagacgac gttgttcgaa gagacgtacc
    28561 ggaaccccgc tgtcgacgcc gtggcccgcg cgctgatgga cgacgagccg ccggacgtcg
    28621 tgcacgtgca tcacctgacg agcctgagca cgggcatcgt cgaggagtgc gcccggcgcg
    28681 acatcccggt gatcgtcacg ctgcacgatt actggctcat gtgccaccgc ggccagctgc
    28741 tggatctcga cctggagagg tgccggggcc cggagtccga ccggtgcgcg gtgtgcgcgg
    28801 ggctggcgga ctcggcccgt ccggccgttc accgagcggc ccggtggctg cgggtgctgg
    28861 acgatcacgt gccccgggcg attgccgatg cgcgccgccg cctggcgtcg ggccttgccc
    28921 ggcgggcggt gccggaatcc gccgccgcgg aggcggagcg ccggatctcc cacatgcggt
    28981 cggtgtgcgt cggcgccgcc cacttgctgg ccccgtcgaa ggcgctgctc gagcagttcg
    29041 tcgcgttcgg ggtgccgcgc gaacgcctgc agctcgttga gctcgggatc gacacgcggc
    29101 ccttcgcccg gctcaagcgc cagccctcag aacggctgcg catcggcttc gtcggcagcc
    29161 tgctgccctc gaaggcgccg cacctggcga tcgaggcggt ccagggcctg ccgccgcggc
    29221 gcgccgagtt gaccgtcgcc ggcagcctcg gaccgtatca cggcgacgcg agctatgcgt
    29281 ccgcgctccg gccgctcctc cgctcgccgg gcgtccgctg gctcaacggc gtcgcgcatc
    29341 acaaggttcc gtccgtctac gaatccctcg acgtcgtggt cgtgccgtcg atctggctgg
    29401 agaactccct gctcgtcatc agggaggcgt tcgcggccgg cctgccggtt gtcgcgtcga
    29461 acctcggcgc gatggccgag acggtcgcgg acggccgcaa cggcctgctc ttcgagcctg
    29521 gcagctcggc ggacctgcgg cgcgtactgc agcgcctgct cgacgagccc ggcctgctcg
    29581 atcgcctgcg cgaggggatt ccgcgcgtca ggagcatcga cgaggacgcc gcctggacgt
    29641 cggcgctcta ccaggagctg tggtacaggc gccacgggag gccgacgccg ccgcccgagc
    29701 cgtgatagcg cggggcggct ggcggcgcgc cggacgggcc tgctatagtc ggcgctggtg
    29761 cgaggggcat tgacacgggc tcagaggagc gtcccgcccc gggactggct gggcgttgtc
    29821 caggcggcgg cgctgggcct ggtgctggct gcatacgcgt tgatctgttc gtcgttgtcg
    29881 aacagggcgc actcgaccga tttcattagg ttcttctcat cggcgcagct gctgcgcgcc
    29941 ggcgagagcc tctacacgcc gccgcggatc gaggccatcg accaggcgcg ctcaacccat
    30001 ggcgccgcga gccagacgct ccatccgaac ctgaacacgc cggtgctggc gctgctggtc
    30061 gtgcccctca cgttcgccgg ccccgcgcgg ggctacgcga tctggacggc gctctcgctc
    30121 gtggcggggc tgtgcgcctg cgccctggtg tggcgtggcc tccgcccccc cggccagggc
    30181 gcctccggac tgctctggct gtggctcgcc ttcctcgtct acttccccac ctatgcgtcg
    30241 ttcgtgctgg ggcaggtcac ggccggcctg ttcctgctgc tcgccggcgc gtggctggcg
    30301 gcgcggaacg gacgcgaccg gctggccggg gtgctcctgg gcgcggcggt gagcgtgaag
    30361 ctgttcgccg cgctcctgct ggtgtgtttc gtccttcagg ggcgatggcg cgtcgcggcc
    30421 tggtccatcg gcgccgggct gtgcgcggcg ctcgcgacgc tgccatttgc ggggctcgcg
    30481 gcgtggcgcg agtacttcgg cgtcatccgc acggtcacgt ggttcgggaa cagctggaac
    30541 gcctcctacg catcgttcat cacgcggatc ctggggggga cggagaacgc gcccctcgtc
    30601 gacgcgccgg cgctcggacg cgcgatcgtg gcgctctgtt ccgccgtcac gctcctgtgg
    30661 atggcacggc tcacgaggct ggcgtccgtc gaggccaaag gcgaggccct catggctcaa
    30721 gctgcgggct tcgacctcgg gtacgggctg acgctagcgg tcatgctgct cgtgtcgccg
    30781 ctgggatgga tgtactactt cccggtcctg ctgcttcccg gctatgcggt ctggctgctg
    30841 acgcgcggcg ggcgcatgcg gctgctccgg ctggggctcc tcgccgcctg ggggctgagc
    30901 gccgtgccct ccacgatgg
//
LOCUS       DAOSIF010000003        24974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_2230_length_24974_cov_3.886873, whole genome shotgun sequence.
ACCESSION   DAOSIF010000003 DAOSIF010000000
VERSION     DAOSIF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 24974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24974
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_24974_cov_3.886873"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLT35_00225"
     CDS             <1..730
                     /locus_tag="PLT35_00225"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG27676.1"
                     /translation="AQIRRTLAPSSFPLGKTLTMKNVTEHHAEGVGSNVVAWKEGADS
                     ELKREAIVVGAHLDHLGLNPVLMPGAHDNASGVGVALAVAQALARAGVPLKRSVIVIL
                     FGAEEQGVKGSEYYVAHPFMPLKAIKAFVNLESVGRGERIEVGSGRDYPQICAVMERV
                     NERFVHRKLSASTNANVARPRQDAAHFLWAGVPSVSIGTSGAPPLPYPTYHTTRDRWD
                     ILTPEIMEDLARIVFLSVVELANQ"
     gene            727..1593
                     /locus_tag="PLT35_00230"
     CDS             727..1593
                     /locus_tag="PLT35_00230"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOG27677.1"
                     /translation="MTGREPAAVARPVERRASLVRRVPSLFLAHGSPMSVLDREYARS
                     LRAFASKQTRLRAIVVVSAHWRAAGPLRVTSHPKPSLIYDFTGLPGWLYEIAYPCPGD
                     RAVAQMVAVLLERGGFEVRLDPGRGLDHGAWVPLSVAFPEASVPVVQLSMPVDPAPAT
                     MIAMGRALGPLRSEHVLLVGSGGITHNPSRVLPDEDGADAQPWASEFDAWVRERVGEL
                     DVESLGAYRHLGPRAGEAAPTSEHFDPLLFTLGTALPGDVVYDIHEGFRFGSLSMRCF
                     ALTGRRREDRGF"
     gene            1959..3311
                     /locus_tag="PLT35_00235"
     CDS             1959..3311
                     /locus_tag="PLT35_00235"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOG27678.1"
                     /translation="MEGRELPGDGVVTGKGEINGRPAFAFSQDFTVGGGALGSLHAAK
                     IVECLRTALKCGIPVVGFNDGGGARIQEGVEALSGYGQVFYHNTLLSGVVPQVSIIAG
                     PCAGGAAYSPALTDFIIMLEGTAHMFIAGPEVIKAATGESITEEALGGALAHAATAGN
                     IHFIARDEQEAVGLVKALLSYLPLNNLESPPDTPFAEPVVDNAELDDLLPGDSRQAYD
                     VREVIVRLVDADSFFEVQRDFAPNMVIGFARLGGVVVGVVANNPAFLAGAIDINAADK
                     SSRFIRTCNVFNVPIVTLVDVPGFLPGVAQEHGGIIRHGAKMLFTYSASTVPKLTVIL
                     RKAYGGAYLAMCSRDMGADSVLAWPTAEIAVMGPEGAARVLYKKEIEQAADKAAAIAR
                     RVEEYRRQHANPFRAAEAFHLDDIIRPSWTRRLLIERLGILRAKRDIRPQKKHGNLPL
                     "
     gene            3323..3625
                     /locus_tag="PLT35_00240"
     CDS             3323..3625
                     /locus_tag="PLT35_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27679.1"
                     /translation="MNPYFVALVAFLVVMAGMAFLMGLFYALRAAGARIGGAPAEPVS
                     GVNPELLAVVAAAASEALQAPVRLHRVHVHRGPVIERWSRAGRMDIMVSRRVEPKR"
     gene            3622..4044
                     /locus_tag="PLT35_00245"
     CDS             3622..4044
                     /locus_tag="PLT35_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27680.1"
                     /translation="MKKLRITVNGRDYDVTVQVLEDDEQVVTGSTGFLSPLPPVTAPP
                     RSPSSMPPAPPAPPSAGAGLKGDPNAILAPVSGTVQKLYVQAGSRVDAKAPVVLLDAM
                     KMDTYIYAPRAGDVSEVCVNPGDGVQVGDCLIRYTRED"
     gene            4046..5164
                     /locus_tag="PLT35_00250"
     CDS             4046..5164
                     /locus_tag="PLT35_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOG27681.1"
                     /translation="MYEFLQSTGFASLTIREAVMIAVAGLLLWLAIKKGFEPLLLVPI
                     GFGALIANLPLNGLMTPPHGEEIGGLFHYLSYGVRLEIFPPLIFLGLGALTDFGPLLA
                     NPKTLLLGGAAQFGVFGAYLAALGLGFTPEEAGSIGIIGGADGPTSIFLTMKLAPHLL
                     GPIAVSAYSYMALVPLIQPPIMKLLTTREERLIRMEAKFDVPTWVRIIFPIAVTVVGA
                     MLVPAATSLIGLLMFGNLLRECGVTERLTKSAQNELINIITILLGLSLGVTMAGDSFL
                     RWATIKILLLGCVAFALSTAGGVLLGKLMARLPGSPVNPLIGAAGVSAVPMAARVAHV
                     VGQEEDPNNYLLMHAMGPNVAGVIGTAIAAGALLAMLK"
     gene            5232..6713
                     /locus_tag="PLT35_00255"
     CDS             5232..6713
                     /locus_tag="PLT35_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG27682.1"
                     /translation="MPETTPRSRFPRTFWVANVMELFERAAYYGMNSVLAVYLTGPAA
                     RGGLGFAEQSVGFLQSIVYAMTYVLPILGGALADRYGYRRMLMVAFSLLAAGYFATGY
                     TSAYATVFLGLLVMATGSGLFKPIISGTIARSTDDSNSAFGFGIYYWMINLGAFLAPL
                     VVSYLKGFSWQYVFLASSLYCALMLVPTVFAYTDPPRPASTKTLKQVLVGAAEVLGDS
                     RFMLMIVVYSGFWILYFQNFGSVLWYLRDFIDGAPVSRAVSGALAAIGLPVAFRFDAE
                     HVTVINAGTIILLQVLVSRIVKRLPAVPTMVAGMLIGGVGFLLLASSHSPWVFVAGIA
                     VFSIGEMTAHPKYYSFVGLVAPADRKAVYMGYAFLYGVFGSLLGSNLGAFLYTRMLKP
                     AAGAPGAFAASRTFWLMFAALDVLAAIGLILFARAFSADTPETRRKARVVMGGVYAVI
                     LLVGLAFCWVAFSASPVQYRTLVQAIIFLALGAGGLLVGRSRT"
     gene            complement(6816..8108)
                     /locus_tag="PLT35_00260"
     CDS             complement(6816..8108)
                     /locus_tag="PLT35_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOG27683.1"
                     /translation="MDVYRQRVTTAEDAVRRIESGHRVFMTGNCSVPYVLMDALVERA
                     RGLQDVELCCILTFGKAPYSAPDMAGHIRVNTMFISDENRPAVSEGRADFTPCFLSEV
                     PLLFKHGHLPLDATLVHVSPPDEHGFCSYGIEVSSTKAAAQSSRTVIAEVNPRMPRTL
                     GDAFIHVSKLDCIVEVDYPLPTVQMAGTSELAQRIAANVASLIPDGATLQTGIGAVPD
                     SVLRLLSDRKHLGIHTELFSDGVIDLVEKGVIDGEMKSIHPGKIIAGFMFGTQQLYDF
                     VHDNPIIEMHPTEYVNDPFVVAQNDRMVAINSAIEVDLTGQVCSDSIGPRIYSGVGGQ
                     VDMIYGASRSRGGVPIIAMPSTAVVQGQTVSKIVPMLKPGAGVVTSRYLVHYVITEHG
                     IASLYAKTIRQRARALIDIAAPEFRAGLEQKAIELRYL"
     gene            complement(8114..9223)
                     /locus_tag="PLT35_00265"
     CDS             complement(8114..9223)
                     /locus_tag="PLT35_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOG27684.1"
                     /translation="MNIGVPRERQPDEHRVGLTPSAVERLVAAGHHCSIEKHAGLGAG
                     FEDVEYEKAGGRIVYSGEEACRRAELVVKCSKATEEEIGWLNEGSVLMGYLHLAGGRR
                     DRVDALLAKGITAVAYETIQNEDGSLPVQSPMSRLAGMMLPQVAGTLLQNDRGGKGIL
                     LSGLAGLPPAEIAILGAGTAGTAAADLFLGLGSSVTVLDHDLGRLRALEERLRYGPRP
                     VLMVAYESNIRKAVSFADVLIGAVHVPGAKAPIVVTKDMVASMKPRSVIMDIAIDQGG
                     CVETSRPTTHRNPTFLAENVIHYCVPNMASVVARSASQMLNNAALPFVSAVADGGLES
                     ALAAHPALRRGVATHRGRVVNARLAFHLGVPEVTL"
     gene            complement(9386..10291)
                     /locus_tag="PLT35_00270"
     CDS             complement(9386..10291)
                     /locus_tag="PLT35_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG27685.1"
                     /translation="MGFDVVGVGASCVDHVCRLPVFPAAGGAHAKLRMLAQSRSSGGQ
                     TATALATCASLGLRPAYLGAIGDDDDGRRVRRELLERGVNVEHVTVSASPTATATILL
                     DATGDRIVLWYREPGLCYPPDRLPVEVITSARVVHVDDVDEPAAIAAARIASQTGIPV
                     TCDIDHVTPATAELLPLISHPVFAESVPTALTGESDPERALRRLRRVHPGRLVVTLGE
                     RGAMALEGDMLAHMPAFSVSVVDSTGAGDVFRGAYIRALVEAWPLADSLRYANAAAAV
                     SCTKAGAMGGVPTAAEVEALLASRV"
     gene            complement(10390..12075)
                     /gene="ggt"
                     /locus_tag="PLT35_00275"
     CDS             complement(10390..12075)
                     /gene="ggt"
                     /locus_tag="PLT35_00275"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOG27686.1"
                     /translation="MIRRFALAVFVATAVAAVAATWSEPHRATRGMVVSEHAIASEIG
                     VAVLKAGGNAVDAAVATAFALAVVHPAAGNIGGGGFLLYRPASGEPAAYDFRETAPAA
                     ASAAMFLTDGKYDAAKHHESYLSVGVPGTVAGLHLAWQERGTMPWRRLVEPAITLARD
                     GFPLSESLAASLARVLPSMRRHPASMAQFSKSGSPYAPGDTLVQADLARTLERIAADG
                     PAGFYEGETARLIEQDMAAHGGLISRADLKAYRPERRVPLRGTYRGYDIIAMPPVSSG
                     GTALIEMLNVLEGFDLASYGWGSARHVHVAVEAMRRAYADRARHLGDPAFNPSMPIAT
                     LISKEYAASLRATIDLERASVSSPSSFDWPPESGETTTFCVVDAARNAVSLTYTLEAG
                     YGSKIVVAGAGFLLNNEMGDFNAGPGLTDATGLVGTAPNLAAPGKRMLSSMTPTIVAK
                     DGRLFAAAGALGGRTIINTVLQAVLNVIDFGMNAQEAIDAGRFHHQWLPDAVVVERHA
                     LSPDTLAILTSRGHAFRETGALSVAEMIVVAPKDGKLTAGVDARSVDGAAAGY"
     gene            complement(12087..13817)
                     /locus_tag="PLT35_00280"
     CDS             complement(12087..13817)
                     /locus_tag="PLT35_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27687.1"
                     /translation="MIEKGAGAIARAEPRTTAQVRLPDGRIFEAPIGTRVGDVLKAAW
                     PSPARPFMAALVGGRLRELTFPLAGDAEVTPLDASTSDGVRIYRRSLSFLMLVAIEEL
                     HPDAEIFIEHSAATAAGYYCEVRGRRPFAQDELRRIEARMREIVAEDAPILRERVSRE
                     EAMAVYRSRGETDKARLLAHRGREALVLYRLHDRRDYFQGYMAPSSGCLTHFALHALP
                     AGFMLQFPHQGRPHDIAPVKPYPKLFAVFEQASDWLDRLGIRCAGALNDATAGGRLSE
                     ISLVAEALHEAGLAEIAGRIARSRGRVRVVFIAGPSASGKTIFSKRLAVQLLANGIRP
                     MPLALDDYFVDRDRTPRDAKGEYDYESLGALDLDLFEAQLRSLIDGRRTDLPRYDFLT
                     GLREAGQTVTLGPEHVVIVEGIHGLNPVLAQGLAPESVFRVYVSALTQLNLDRHNRVS
                     TSDCRLLRRIVRDSATRGYSASDTLHRWESVLRGEKRHIFPFQENADAIFNSALVHEL
                     AVLRPFAEPLLLQVRPEEPEYVESNRLLSFLMWFRSAPPDHVPDNSILREFIGGSILS
                     ALRLWPDAPA"
     gene            14022..15011
                     /locus_tag="PLT35_00285"
     CDS             14022..15011
                     /locus_tag="PLT35_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG27688.1"
                     /translation="MSSPAPASDRRTFLLRVLRLGGASAAAVGLGAYLSGRSRRPEER
                     AASQVALDHRVADRPDLPELAVARGESAEPAALVRRAIADMGGMGRFVARGATVVIKP
                     NVAWDRLPEYAATTNPEIVAQLVRLCREAGAGAVIVTDVSCNEPEACFERSGIGAAAR
                     AAGAEVVLPQTRFFKTIDLGGQVLREWPVLEPFLEADTLINVPIAKHHSLTGVTLGMK
                     NWYGLIGGDRNRLHQRIHESLVDLTDFAKPSLTVMDAYRVLMRNGPTGGNVDDVEAKG
                     TVLASTDPVALDAYAAKAWWDLDVPRLRYLRIAEARGVGRPAFESLRTVVSNL"
     gene            complement(15124..16014)
                     /locus_tag="PLT35_00290"
     CDS             complement(15124..16014)
                     /locus_tag="PLT35_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG27689.1"
                     /translation="MTVRNPDLAVVITAYRARQVVGACLDSLRGQRTSRTFETLLVDS
                     SDDGTAAYVRGAYPEVAVLTSPTRLHAGAARNLAIPHARAPLIAFLDADCTVGPAWVD
                     LVCAAHERSELLVGGAVENAPTRSLVSWAYYFCEFNLWVPSPRGRYVDEVPGCSLSMK
                     REAFERYGPFVEGSYSSDTAFQWKTRADGHRAWFTPAIRVVHHSPAGLGRFLGHIVEH
                     RRGYARVLCREKQLSVAVRLGRALASPVTPVLMLGVTLWRLRRCPRYLLWAVAASPLV
                     LLGYAARSWGEFRGYLRGRD"
     gene            complement(16275..16439)
                     /locus_tag="PLT35_00295"
     CDS             complement(16275..16439)
                     /locus_tag="PLT35_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27690.1"
                     /translation="MYDMIAHAKAKSVKASSSTNGLLFARAGHAEALVRSGLDTIIFA
                     PDGTTLRTYE"
     gene            complement(16470..16622)
                     /locus_tag="PLT35_00300"
     CDS             complement(16470..16622)
                     /locus_tag="PLT35_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27691.1"
                     /translation="MGPASAESGSLRCALYPVNVGFTRPQGVTDAALFRKVLDEAGPC
                     AFTLRL"
     gene            complement(17370..18548)
                     /locus_tag="PLT35_00305"
     CDS             complement(17370..18548)
                     /locus_tag="PLT35_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27692.1"
                     /translation="MATQKRGTSESLDALAARCCPRQSVEEVRCNLVPPTRGRRLSAD
                     AARALAEDRVAAVVSRACEWGSLGSGWEVGDYRYLVLSFVLKGNVEFYVQFWTEPEEP
                     VLVEACSGAWNSASRPYIGPPQRAALRRLGYRVGGRARNFGKRWPLSPLADARALAAE
                     LVTILVDVFGYRAASALELAYRAGRRTVDAPVFPALGRDDVERMLRMGGVRLIDARPA
                     GRVSPGVRRRLVHAGEPFRLTVELNGEVGKEPFRYEAMRFITPIPGGLGVSDAQLAAI
                     ARACPFLRLLRDDEGEVLAMYDVPVAGTTVRWFLLTLHVVGVMRARAVSMVQEALAAR
                     HGLPRGRAGGGGALGDGVGAIGQDSPDPEKAAALDEYPDQTEADSSTGPGRSRRFVVH
                     "
     gene            18737..19237
                     /locus_tag="PLT35_00310"
     CDS             18737..19237
                     /locus_tag="PLT35_00310"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOG27693.1"
                     /translation="MYQTIAEFVDVWRSESENTHKVMCELTDASLAQRVAPEGRTLGR
                     IAWHIVQTLGEMGGRAGLSVEGADEKTPQPATAAAIADAYRAGASALEKALPAAWSDA
                     DLAGEIELYGEKWTRAKTLHVLLVHEIHHRAQMTVLMRQAGLRVPGVYGPAREEWTAY
                     GMPAQE"
     gene            19240..19812
                     /locus_tag="PLT35_00315"
     CDS             19240..19812
                     /locus_tag="PLT35_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOG27694.1"
                     /translation="MPFCDRARRAAAAVGAALTLLPAASAPAVGAGSAAGCESVRAQE
                     AFSGRVVGILDGDSLVVLRDGRQVTVRLHGIDAPESGQAYGNVARRALANLAFGKTVE
                     VAVRDVDRYKRLVSRVTAEGADVGLEMVRAGFAWHYARYLRDEAYAAAEREARAARRG
                     LWLDRSPVAPWEWRANSKHVRPRSGSRRRN"
     gene            complement(19809..20516)
                     /locus_tag="PLT35_00320"
     CDS             complement(19809..20516)
                     /locus_tag="PLT35_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOG27695.1"
                     /translation="MRTIGLIGGMSWESTVPYYQIINRVVGARLGGLHSAKIVLYSVE
                     FAEIEAMQREGRWEDSGKVLAGAARAVRRAGADFIVLCTNTMHKVAPQIEAAVDVPLL
                     HIADATAARVKAAGVRRVGLLGTRFTMEQDFYRGRLEARHALSVLTPPDEARALVHGV
                     IYGELCIGRLRPESREGFRQVVDELVGRGAEGVILGCTEIGLLLRPEDAAVPLWDTAS
                     IHAEAAADYALGGELPS"
     gene            20793..23453
                     /locus_tag="PLT35_00325"
     CDS             20793..23453
                     /locus_tag="PLT35_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG27696.1"
                     /translation="MTTRQAITPAGAQSIFEGRVYGVAFGTTSDTLWVLTSDDLHELD
                     WAANAVRQRIDTGLTPGPQSLCFDRGGAQALVAGASGIRTTGKPPAVVLGAALPAARP
                     EPGQRDVTPASPRPAPPPDRRSEAALPALAPIADGLGWHIAGAVSAAPRARPDGRRLA
                     LVPLIYDNAVAVIDVEGSAILGSVRTEVAPFGTVVDDTGTVAWASNWGGRVPEAGERS
                     APIGDYEGADRILVDERGVAALGTIVRIDVPSLQVTDTIEVGPHPTALAWDQARERLY
                     VALGNDDAVEVIDTSRRRKAGRVEISPFGRGIRGTAPTAVALAADGSRLYVACGGINA
                     VAVIDTAALSVLGLIPTAWYPDSLALSPDGRMLAVGALLGVGSGWRRPATPSLTLGMP
                     APTRRYVHATRGSVSVLPVPTDGELVNYTAAVAENNRLPLAARESAPPARTARDARGR
                     QPRPAPTAVPLTAGDPSLIEHVVYIIKENRTYDQMFGDLPNGNGDPALVLFGEDIAPN
                     QRRLARQFVLLDNFFATGGNSGDGHQWVTQADESDYVIWPGYLGRSYPKDGSDALAPA
                     QGGFIWDAARKMRKSVRVFGEYLPAERRVAASARADLLREWAKGADFTNRWKPTPHVQ
                     GLKGITASDFPSYTLAIPDVIRADLFLRDLRAWQRSGRMPNLVIMLLPSNHTYGTSPG
                     LSTPQAMVADNDLALGRVVEGLTRSRFWPRMAIFVVEDDAQDGVDHVDGHRTVAQVIS
                     PYSRRGHVDSTFYAQPSILKTIELILGLQPLSIFDRIANDMRASFTDQADTTPYTAVV
                     PKQPLHEVNPPASALTGAAREAALASARMRFDEPDAAPTDRLNRCIWHAIKGWDVPYP
                     GVRRAVFAPLSVDLDDDERQDARPDGRDRR"
     gene            23582..>24974
                     /locus_tag="PLT35_00330"
     CDS             23582..>24974
                     /locus_tag="PLT35_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27697.1"
                     /translation="MTASTLRHAVLTFALALPAALVLLAVSLAAQDRDRPMRVPQDPG
                     VITTRQAITPAGVQSVFEGRVYGVAFGASPNRLWVLTASDVYELDWATNTVRQRVDTG
                     LNAGLQGLRFDRAAARALVAGASRARAAGKPPEVRLEAAAASLDRVPAVSDGAAAPAP
                     PVAPATAPAPGATAPITPVTPMGRVELASPLSLELVAGGLGSHIAGALAVAPRPRADG
                     RRVALVPLVASNQVSVVDVGSGAVLGAVKTDIAPFAAVVNDDGTTAWVSCWGGRVPLE
                     GELAATTGENAGADRVVVDARGVAASGTVARIDLDAMRVTGTLEVGLHPTALAWDEAR
                     ARLYVALGNDDAVAVVDTEQRVLAGRIEVRPFGRDVRGIAPTALALSADGGRLYAACG
                     GINAIAVIETAGRSLAGLIPTAWYPDSLALSPDGAMLAVGALLGAGSGWRRPATPSFS
                     LGLPDTSRRYVHAH"
BASE COUNT         3539 a         9095 c         8679 g         3661 t
ORIGIN      
        1 cgctcagatc cggcggacgc tcgccccgtc gtcgttcccg ctgggcaaga cgctgacgat
       61 gaagaacgtg accgagcacc atgccgaagg tgtcggctcg aacgtcgtcg cgtggaagga
      121 gggggccgac tccgagctga agcgcgaagc catcgtcgtg ggcgcccatc tcgaccacct
      181 ggggctcaac cccgtcctga tgccgggcgc gcacgacaac gcgtctggcg tgggtgtcgc
      241 gctggcggtg gcgcaggcgc tcgcgcgggc gggcgttccg ctcaagcggt ccgtgatcgt
      301 catcttgttc ggggccgagg agcagggtgt gaagggatcc gagtactacg tggcgcaccc
      361 gttcatgccg ctgaaggcga tcaaggcctt cgtcaacctc gagagcgtcg ggcgcgggga
      421 gcgcatcgag gtcggctccg gccgggacta cccgcagatc tgcgccgtga tggaacgcgt
      481 caacgagcgc ttcgtccacc ggaaattgag cgcgagcacg aacgccaacg tggcgcggcc
      541 gcgccaggac gccgctcact ttctgtgggc gggcgtgccg agcgtctcga tcggcacgtc
      601 cggcgcgccg ccgctcccct atccgaccta ccacacgacg agagaccggt gggacatcct
      661 gacgcctgag atcatggagg atctcgcgcg gatcgtgttc ctgtcggtgg tggagctcgc
      721 gaaccaatga cggggcggga gcccgccgcc gtggcgcggc ccgtcgagcg ccgcgcgtcg
      781 ctggtccgcc gcgttccgag cctgttcctg gcgcacggct ctccgatgtc ggtcctcgac
      841 cgggagtacg cgcggtcgct gcgggccttc gcgtcgaagc agacgcggct gagggccatc
      901 gtggtcgtgt cggcgcactg gcgggcggcc ggccccttgc gcgtcacgag ccatccgaag
      961 ccgagcttga tctacgactt caccgggctt cccggctggc tctacgagat cgcgtacccg
     1021 tgtccgggcg atcgcgccgt ggcgcagatg gtggcggtcc tgctcgagcg gggcggattc
     1081 gaagtcaggc tcgatccggg ccggggcctc gatcacggcg cgtgggtgcc gctcagcgtg
     1141 gcgtttcccg aggcgtcggt gcccgtcgtg cagctgtcga tgccggtgga tccggcaccg
     1201 gccaccatga tcgcgatggg ccgcgcgctc gggccgctcc ggtcggagca cgtgctgctc
     1261 gtcggttcgg gcggcattac gcacaacccg tcgcgggtcc tgccggacga ggacggcgcg
     1321 gacgcccagc cgtgggcctc ggagttcgac gcctgggttc gcgagcgcgt cggcgagctg
     1381 gacgtggagt cgctcggcgc ctaccgccac ctcgggccgc gtgcgggcga ggcggcaccg
     1441 acgtcggagc acttcgatcc gctcctgttc acgctgggaa cggcgctgcc cggcgacgtc
     1501 gtgtacgaca tccacgaggg attccgcttc gggtctctct cgatgcgctg cttcgcgctg
     1561 acaggccgcc ggcgcgagga ccggggtttc tgatgcggcc gccggcggag ccgccccgcc
     1621 ggtttgcggt gcgtgcgggt ttgggctaat atgcggggtc cgagtccccc gtcccggagc
     1681 gcccttacgc ggtcctttct cgccgtcatc ccggcagcaa ctgcgttcgc gcgtcatcgg
     1741 cccgccggac cctccggccg gggctggccg cgcgggagaa cctgacgtga aaaccgacct
     1801 ccacgaactg gacgctcgcc gcgccgcact gcaggccggg ggcggcgcgg acaagatcgc
     1861 ccggcagcac cagctcggca agctgagcgc gcgggagcgc ctggcgctgc tgttcgacgc
     1921 cgacagcttc gtggagttcg gcttgccggt gccgggagct ggaaggccgg gagctccccg
     1981 gcgacggcgt cgtgacgggg aagggcgaaa tcaacgggcg gccggcgttc gcgttctcgc
     2041 aggacttcac cgtcggcggc ggggcgctgg gctcgctgca cgcggcgaag atcgtcgagt
     2101 gcctgcggac ggcgctcaag tgcggcatcc ccgtcgtcgg gttcaacgac ggcggcgggg
     2161 cgcgcatcca ggaaggcgtc gaggcgctgt cgggctacgg gcaggtcttc taccacaaca
     2221 cgctcctctc gggcgtggtg ccgcaggtct cgatcatcgc cggtccgtgc gccggcggcg
     2281 cggcctactc ccccgccctg accgacttca tcatcatgct cgagggcacc gcccacatgt
     2341 tcatcgcggg gcccgaagtg atcaaggcgg ccacgggcga gtcgatcacc gaggaggccc
     2401 tcggcggcgc cctcgcgcac gccgcgacgg cgggcaacat ccacttcatc gcgcgcgacg
     2461 agcaggaagc ggtgggcctc gtgaaggcgc tgctctcgta cctgccgctg aacaacctcg
     2521 agtcgccgcc ggacacgccg ttcgccgagc cggtcgtgga caacgccgaa ctggacgatc
     2581 tcctgcccgg agactcgcgc caggcctacg acgtgcgcga ggtcatcgtc cggctcgtgg
     2641 acgccgactc gttcttcgag gtgcagcggg atttcgcacc gaacatggtc atcggcttcg
     2701 cgcggctcgg cggcgtggtc gtcggcgtgg tggcgaacaa cccggccttc ctggccggcg
     2761 ccatcgacat caacgccgcc gacaagtcgt cgcgcttcat ccggacgtgc aacgtgttca
     2821 acgtgccgat cgtgacgctg gtggacgtgc ccggcttcct gcccggcgtg gcgcaggagc
     2881 acgggggcat catccggcac ggcgccaaga tgctgttcac ctactcggcg tccacggtgc
     2941 cgaagctgac ggtcatcctc cgcaaggcgt acggcggcgc gtacctggcg atgtgctcgc
     3001 gcgacatggg cgcggactcg gtgctggcct ggcccacggc ggagatcgcg gtgatgggac
     3061 cggaaggcgc ggcgcgggtg ctctacaaga aggagatcga gcaggcggcg gacaaggccg
     3121 cggcgatcgc ccggcgggtc gaggagtacc ggcgccagca cgcgaacccg ttcagggcgg
     3181 ccgaggcctt ccacctggac gacatcatcc ggccgtcatg gacgcggcgg ctgctcatcg
     3241 agcggctcgg catcctgcgc gcgaagcgcg acatccggcc gcagaagaag cacggcaacc
     3301 tgccgctgta gaagggccgc cgatgaaccc gtacttcgtc gcgctcgtgg ccttcctggt
     3361 ggtcatggcc gggatggcct tcctgatggg gctcttctac gccctgcggg cggctggcgc
     3421 caggatcggc ggcgcgccgg cggagcccgt gtccggcgtg aaccccgagc tgctggccgt
     3481 tgtcgcggcc gcggcaagcg aagcgctgca ggcccccgtg cgcctgcacc gcgtgcacgt
     3541 gcaccgcggc cccgtgatcg agcgctggtc gcgcgccggc cggatggaca tcatggtgtc
     3601 ccgccgtgtg gagcccaaga gatgaagaag ctgcgcatca ccgtcaacgg ccgcgactac
     3661 gacgtcacgg tccaggtcct cgaggacgac gagcaggtgg tcaccggctc gaccgggttc
     3721 ctgtcgccgc tcccgccggt gacggcgccg ccgcggtcgc cgtccagcat gccgcccgcc
     3781 cctccggcgc cgccgtcggc cggcgcgggg ctcaagggcg acccgaacgc catcctggcg
     3841 ccggtgtccg gcacggtgca gaaactgtac gtccaggccg gcagccgcgt cgacgccaag
     3901 gcgcccgtcg tgctgctcga cgcgatgaag atggacacgt acatctacgc gccgcgcgcc
     3961 ggcgacgtgt cggaggtatg cgtgaatccc ggcgacggcg tgcaggtggg cgactgcctc
     4021 atccggtaca cgcgggaaga ctgacgtgta cgagttcctc cagtccaccg ggttcgcgag
     4081 cctgacgatc cgcgaggcgg tcatgatcgc ggtcgccggc ctgctgctct ggctggccat
     4141 caagaagggc ttcgagccgc tgctgctggt gcccatcggc ttcggggcgc tcatcgcgaa
     4201 cctgcccctc aacgggctga tgacgccgcc gcacggcgag gagatcggcg ggctgttcca
     4261 ctacctgtcg tacggcgtcc gcctcgagat cttcccgccg ctcatcttcc tcggcctggg
     4321 ggcgctcacc gacttcgggc ctctgctcgc gaacccgaag acgctgctcc tcggcggagc
     4381 ggcgcagttc ggcgtcttcg gcgcgtacct cgcggcgctg ggcctgggct tcacgcccga
     4441 ggaggccggc tcgatcggca tcatcggcgg cgcagacggc ccgacgtcga tcttcctgac
     4501 catgaagctc gcgccgcacc tgctcgggcc gatcgccgtc tcggcctact cctacatggc
     4561 gctcgtcccc ctcatccagc cgccgatcat gaagctcctc acgacgcgcg aggagcggct
     4621 gatccggatg gaggcgaagt tcgacgtgcc gacgtgggtg cgcatcatct tccccatcgc
     4681 cgtcaccgtc gtcggcgcga tgctcgttcc ggcggcgacc tcgctcatcg gcctcctgat
     4741 gttcggcaac ctgctgcgcg agtgcggcgt gacggagcgg ctgacgaagt cggcccagaa
     4801 cgagctgatc aacatcatca cgattctcct cggcctctcg ctgggcgtga ccatggccgg
     4861 cgactcgttc ctgcgctggg cgaccatcaa gatcctcctg ctcggctgcg tggcgttcgc
     4921 cctctcgacg gcgggcggcg tgctcctcgg caagctcatg gcgagactgc ccggcagccc
     4981 ggtcaacccc ctcatcggcg cggcgggcgt gtcggcggtc cccatggcgg cccgggtcgc
     5041 ccacgtggtg ggccaggaag aagacccgaa caactacctc ctgatgcacg ccatgggccc
     5101 gaacgtcgcc ggcgtgatcg gcaccgccat cgccgcgggc gcgctgctcg cgatgctcaa
     5161 gtaggatcgg tcctgcggta gaatccacgt cctggcgcgg cgcccgccgc gccaagcgcg
     5221 aggggaggcg catgcccgag acgacgccac gcagcaggtt cccgcgcacg ttctgggtcg
     5281 ccaacgtcat ggagctcttc gagcgggcgg cctactacgg catgaattcc gtgctggccg
     5341 tctacctgac cggccccgcc gcccggggcg gcctcggctt cgccgagcag tccgtcggct
     5401 tcctccagag catcgtgtac gcgatgacct acgtgctgcc gatcctcggc ggcgcgctcg
     5461 cggaccgcta cggctaccgg cgcatgctga tggtcgcctt ctcgctcctg gcggccggct
     5521 acttcgccac cggctacacc tccgcctacg cgacggtctt cctcgggctg ctcgtcatgg
     5581 ccaccggatc ggggctcttc aagccgatca tctcgggcac gatcgcgaga tccaccgacg
     5641 acagcaactc cgccttcggc ttcgggatct actactggat gatcaacctc ggggcgttcc
     5701 tcgctccgct ggtcgtcagc tacctgaagg gcttctcctg gcagtacgtc ttccttgcct
     5761 cgtcgctgta ctgcgcgctg atgctcgtcc cgacggtctt cgcctacacg gacccgccga
     5821 ggcccgccag cacgaagacg ctcaagcagg tcctcgtggg cgcggccgag gtcctcggcg
     5881 actcgcgctt catgctgatg atcgtcgtct actcggggtt ctggatcctg tacttccaga
     5941 acttcggatc ggtgctgtgg tacctgcgcg acttcatcga cggcgcgccc gtcagccggg
     6001 ccgtgtcagg cgcgctcgcg gccatcgggc tgcccgtcgc gttccggttc gacgccgagc
     6061 acgtcaccgt catcaacgcc ggcaccatca tcctgctgca ggtgctggtc agccgcattg
     6121 tcaagcgcct gcccgccgtg ccgacgatgg tcgcgggcat gctgattggc ggcgtcggtt
     6181 tcctcctgct cgcctcgtcc cacagcccct gggtgttcgt cgcgggcatc gccgtgttct
     6241 cgatcggcga gatgaccgcc caccccaagt actacagctt cgtcggcctg gtggcgccag
     6301 ccgaccgcaa ggccgtctac atgggctacg cgttcctcta cggcgtgttc ggatcgctgc
     6361 tgggctcgaa cctcggggcg ttcctctaca cgcggatgct gaagcccgcg gccggcgcgc
     6421 ccggggcgtt cgcggcgagc cggacgttct ggctgatgtt cgccgcgctg gacgtcctcg
     6481 ccgccatcgg cctgatcctg ttcgcgcgcg cgttctcggc ggacacgccg gagacgcggc
     6541 ggaaggcgcg cgtcgtcatg ggcggcgtgt acgccgtcat cctgctcgtc gggctggcgt
     6601 tctgctgggt ggcattctcg gcgtcccccg tgcagtaccg gacgctcgtg caggcgatca
     6661 tcttcctggc cctgggcgca ggcggcctgc tcgtgggccg gtcgcgcacg taggcgcgcc
     6721 attggctccg tcgccacggc gcgcgcggcg gccgctccgg cggcagaagc gccggcgcgc
     6781 cctccgccgc cccgggcgtc cccgcccgcg tgcccctaca ggtatctgag ctcgatggcc
     6841 ttctgctcca gcccggcgcg gaactccggc gccgcgatgt cgatgagggc ccgcgcgcgc
     6901 tgcctgatgg tcttcgcgta cagggacgcg atcccgtgct cggtgatgac gtagtgcacc
     6961 aggtagcggc tcgtgacgac gccggcgccc ggcttcagca tcgggacgat cttgctgacc
     7021 gtctgcccct gcacgaccgc cgtggacggc atggcgatga tgggcacgcc gcccctgctt
     7081 cgggacgcgc cgtagatcat gtccacctgc ccgccgacgc cgctgtagat gcgcgggccg
     7141 atgctgtcgc tgcacacctg cccggtcagg tccacctcga tcgcggagtt gatcgccacc
     7201 atccggtcgt tctgggcgac cacgaacgga tcgttcacgt actcggtcgg atgcatctcg
     7261 atgatagggt tgtcgtgcac gaagtcgtag agctgctgcg tgccgaacat gaaccccgcg
     7321 atgatcttgc cggggtggat cgacttcatc tcgccgtcga tcacgccctt ctcgaccagg
     7381 tcgatgacgc cgtccgagaa gagctccgta tggatcccca gatgcttgcg atccgacagc
     7441 agccgcagca ccgagtcggg cacggcgccg atgcccgtct ggagcgtcgc gccgtccgga
     7501 atgaggctcg ccacgttcgc ggcgatccgc tgcgccagtt ccgatgtgcc ggccatctgg
     7561 accgtcggca gcgggtagtc cacctcgacg atgcagtcca gcttggagac gtggatgaag
     7621 gcgtcgccca gggtcctcgg catcctcggg ttgacctccg caatcacggt ccgagacgac
     7681 tgcgcggccg ccttcgtcga gctcacctcg atgccgtagg agcagaagcc gtgctcgtcc
     7741 ggcgggctga cgtgaaccag cgtcgcgtcg agcggcaggt gcccgtgctt gaacaggagc
     7801 ggcacctccg acaggaagca cggggtgaag tccgcgcgcc cctcgctgac agccgggcgg
     7861 ttctcgtcgc tgatgaacat cgtgttgacg cggatgtgcc ccgccatgtc cggcgcggag
     7921 tacggcgcct tgccgaaggt gaggatgcag cacagctcca cgtcctgcag gcccctggcg
     7981 cgctcgacga gcgcgtccat cagcacgtac ggcaccgagc agttcccggt catgaagacg
     8041 cggtgcccgg actcgatgcg cctgacggcg tcctcggcgg tcgtcacccg ctggcgatag
     8101 acgtccatcc agctcacaac gtcacctccg gcacgcccag gtgaaacgcc agcctggcgt
     8161 tcacgacccg cccccggtgc gtcgcgacgc cccgcctcag cgccggatgc gcagcgagcg
     8221 cgctctcgag cccgccgtcg gccacggcag acacgaacgg cagggccgcg ttgttcagca
     8281 tctgcgacgc ggaccgcgcc acgaccgacg ccatgttcgg cacgcagtag tggatgacgt
     8341 tctccgcgag gaacgtcggg ttgcggtgcg tggtcggacg gctcgtctcc acgcagccgc
     8401 cctggtcgat ggcgatgtcc atgatgacgc tccgcggctt catcgaggcc accatgtcct
     8461 tcgtcaccac gatcggcgcc ttcgcccccg gcacgtgcac cgcgccgatg agcacgtcgg
     8521 cgaagctcac ggccttgcgg atgttcgact cgtaggcgac catcaggacc ggacggggcc
     8581 cgtaccggag ccgctcctcg agcgcgcgga ggcgcccgag atcgtggtcg agcaccgtga
     8641 cgctcgaccc gaggccgagg aacaggtcgg cggcggccgt gcccgcggtg cccgccccga
     8701 ggatggcgat ctccgccggg ggcagacccg ccaggccgga caggaggatc cccttgccgc
     8761 cgcggtcgtt ctgcagcagc gtgcctgcca cctgcggcag catcatgccc gccagccggc
     8821 tcatcggcga ctgcaccggc agcgacccgt cctcgttctg gatggtctcg taggcaacgg
     8881 ccgtgatgcc cttggccagg agcgcgtcca cccggtccct gcggcccccg gccaggtgca
     8941 ggtagcccat cagcacggac ccctcgttca gccagccgat ttcttcttcg gtggccttcg
     9001 agcacttcac gaccagctcg gcgcgccggc acgcctcctc cccggaatac acgattcggc
     9061 cgccggcctt ttcgtattcg acgtcctcga atcccgctcc cagcccggcg tgtttctcga
     9121 tggagcagtg atggccggcg gcgacgagcc gctcgacggc ggatggcgtc aggccgacgc
     9181 ggtgctcatc cggttgccgc tctcgaggga ctccgatgtt catggatcct cctgctgaca
     9241 ggccgccccg gcgcccaggc cgcgtcccag gtcggccatt gtagccgcga acgtcgatgc
     9301 gcggcaggcc ggcccggcag cccgcgcgcg ggtcccccgc ggacccggcg cgctgcccgg
     9361 cgcagcctcc ccgccgctct cctcctcaca cccggctggc caggagcgcc tcgacttcag
     9421 cggccgtcgg cactccgccc atggcgccag ccttcgtgca gctgaccgcc gcggcggcgt
     9481 tcgcgtaccg cagcgaatcg gccagcggcc aggcctcgac gagcgcgcgg atgtaggcgc
     9541 cccggaacac gtcgcctgcg cccgtcgagt ccacgaccga caccgagaag gccggcatgt
     9601 gcgccagcat gtcgccctcg agggccatgg cgccccgctc gccaagcgtc accaccagcc
     9661 ggcctggatg gacccgcctg agccggcgca gcgcgcgctc ggggtccgac tcgccggtca
     9721 atgccgtcgg cacgctctcg gcgaacacgg ggtggctgat gaggggcagc agctcggcgg
     9781 tggccggcgt cacatggtcg atgtcgcagg tgaccgggat gcctgtctgg ctggcgatcc
     9841 tggccgccgc aatggccgcc ggctcgtcca cgtcgtccac gtgcacgacg cgggccgacg
     9901 tgatgacctc gaccgggagg cggtcgggcg ggtagcacag ccccggctcc cggtaccaga
     9961 gcacgatgcg gtccccggtc gcgtcgagga ggatcgtggc agtcgccgtc ggcgacgcgc
    10021 tgacggtgac gtgctcgaca ttgacgccgc gctccagcag ttcgcggcgg accctccgcc
    10081 cgtcgtcgtc gtcgccgatg gcgcccaggt acgccggccg gaggccgagg ctcgcgcagg
    10141 tggcgagcgc ggtggccgtc tgcccgccgg atgaccggga ctgggccagc atgcgcagct
    10201 tggcatgcgc gccgcccgcc gcggggaaga ccggcagccg gcagacgtgg tccacgcagc
    10261 tcgccccgac gccgacaacg tcgaacccca tgtcgcctcc tgcgggaatc gggctccgat
    10321 gtccgtcgcc ctgcccgtcg cgcggcgggg ccaggccgcg agtccgggac gggattcgag
    10381 gccggcccct cagtacccgg ctgccgcgcc gtccaccgat ctcgcgtcca cgccggccgt
    10441 cagcttgccg tctttcggcg ccacgacgat catctcggcg acgctcagcg cgccggtctc
    10501 cctgaacgcg tggccgcgtg acgtcaggat ggccagtgtg tcaggggaca gggcgtggcg
    10561 ctcgaccacg acggcatccg gcagccactg gtggtggaac cgcccggcgt cgatcgcctc
    10621 ctgggcgttc atcccgaagt cgatcacgtt gagcaccgcc tgcaggaccg tgttgatgat
    10681 cgtccgcccg cccagtgcgc ccgccgcggc gaacaacctc ccgtccttcg cgacgatcgt
    10741 cggcgtcatg ctcgacagca tgcgcttgcc cggcgccgcg aggttcggcg ccgtgccgac
    10801 aaggcccgtc gcatcggtca gcccggggcc ggcgttgaag tcgcccattt cgttgttcag
    10861 caggaacccc gcgccggcga cgacgatctt cgacccgtac cccgcctcca gcgtgtacgt
    10921 gagcgacacg gcgttgcggg ccgcgtccac cacgcagaag gtggtggtct cgccggactc
    10981 ggggggccag tcgaacgacg acggcgagga caccgaggcc cgctccaggt cgatcgtcgc
    11041 gcgcagcgac gccgcgtact ccttcgagat cagcgtggca atcggcatcg acggattgaa
    11101 tgccgggtcg ccgaggtggc gcgcccgatc ggcgtacgcc cgccgcatcg cctcgaccgc
    11161 cacgtgcacg tgccgggcgg agccccagcc gtaggacgcg aggtcgaacc cctcgagcac
    11221 gttgagcatc tcgatgagcg ccgtcccgcc ggagctgacg ggcggcatcg cgatgatgtc
    11281 gtacccgcga taggtccctc gaaggggcac gcgccgctcg ggccggtagg ccttcagatc
    11341 ggcccggctg atgaggcccc cgtgggcggc catgtcctgc tcgatgagcc gcgccgtctc
    11401 tccctcgtag aacccggcgg ggccgtcggc cgcgatccgc tcgagcgtcc gggccagatc
    11461 ggcctgcacg agcgtgtcgc ccggcgcgta gggcgagccg gacttcgaga actgggccat
    11521 cgaggccggg tgccgccgca tcgacgggag cacgcgggcg agcgaggcgg cgagcgattc
    11581 gctgagcggg aaaccgtcgc gcgcgagcgt gatcgccggc tcgaccagcc gccgccaggg
    11641 catcgtgccc cgctcctgcc acgccaggtg cagcccggcg accgttcccg gcacgcccac
    11701 cgacaagtag ctctcgtggt gcttcgcggc gtcgtacttc ccgtcggtga gaaacatcgc
    11761 cgccgacgcc gcagccggcg ccgtttcccg gaagtcgtac gccgccggct cgcccgatgc
    11821 cggtcggtag aggaggaagc cgccgccgcc gatgttcccg gccgccgggt ggaccaccgc
    11881 cagggcgaac gccgtcgcca cggcggcatc gacggcgttg ccgccggcct tcagcaccgc
    11941 gacgccgatc tcggaggcga tcgcgtgctc cgacacgacc atgccgcgcg tcgcgcggtg
    12001 cggctcggac caagtcgccg ccacggccgc aaccgcggtg gccacgaaca cggcgagtgc
    12061 gaacctgcgg atcatccggc cccctctcac gccggcgcgt cgggccagag gcgaagcgcc
    12121 gacagaatgg atcccccgat gaactcgcgc aggatcgagt tgtcgggcac gtggtccggc
    12181 ggcgccgacc ggaaccacat caggaacgac aggaggcggt tcgactcgac gtactcgggc
    12241 tcctcgggcc tcacctgcag cagcagcggc tcggcgaacg gccgcagcac ggccagttcg
    12301 tgcaccagcg ccgaattgaa gatcgcgtcg gcgttctcct ggaacgggaa gatgtgccgc
    12361 ttctcgcccc gcaggaccga ctcccaccga tgcagcgtgt ccgaggcgga gtagccgcgc
    12421 gtcgccgagt cgcggacgat ccggcgaagg agccggcagt cggacgtgga cacgcggttg
    12481 tgccgatcca ggttcagctg cgtcagcgcc gacacgtaca cgcggaacac ggactccggc
    12541 gccagcccct gcgccagcac cgggttcagc ccatggatcc cctcgacgat gacaacgtgc
    12601 tccggcccga gcgtcaccgt ctggcccgcc tcgcgcaggc ccgtcaggaa gtcgtagcgc
    12661 ggcaggtccg tccggcggcc gtcgatgagc gaccgcagct gcgcttcgaa caggtcgagg
    12721 tcgagggcgc cgagcgactc gtagtcgtac tcgcccttcg cgtcgcgcgg cgtgcggtcg
    12781 cgatcgacga agtagtcgtc gagggcgagg ggcatcgggc ggatgccgtt cgcgagcagc
    12841 tgcaccgcca gccgcttgga gaaaatcgtc ttacccgacg cgctcgggcc ggcgatgaac
    12901 acgacgcgca cgcgcccgcg gctccgcgcg atccgcccgg cgatctccgc cagccccgcc
    12961 tcgtgcaggg cctcggccac cagcgagatc tccgacaggc ggccgccggc cgtcgcgtcg
    13021 ttcagggccc cggcgcagcg gatcccgagg cggtccagcc agtcgctcgc ctgctcgaac
    13081 acggcgaaga gcttcggata gggcttgacc ggcgcgatgt cgtgcgggcg gccctggtgc
    13141 gggaactgca gcatgaagcc cgccggcagc gcgtggagcg cgaagtgcgt caggcagccc
    13201 gacgacggcg ccatgtagcc ctggaagtag tcgcgccggt cgtgcaggcg gtagagcacc
    13261 agcgcttcgc gcccccggtg cgcgaggagc cgcgccttgt ccgtttcgcc ccgcgaacgg
    13321 tagacggcca tcgcctcctc gcgcgacacg cgctcccgga ggatcggcgc gtcctcggcg
    13381 acgatctccc gcatgcgggc ctcgatccgg cgaagctcgt cctgcgcgaa ggggcggcgg
    13441 ccgcgcactt cgcagtagta gcccgcagcg gtggccgccg agtgctcgat gaagatctcc
    13501 gcatccgggt ggagttcctc aatcgccacc agcatgagga acgacaggga gcgccggtag
    13561 atccgcacgc cgtcggaggt cgacgcgtcg agcggcgtca cctcggcgtc gcccgcgagg
    13621 ggaaaggtga gctcgcgcag ccggccgccg acgagcgccg ccatgaacgg ccgcgccggc
    13681 gacggccacg cggccttcag cacgtccccg acgcgcgtgc cgatcggcgc ctcgaagatc
    13741 cgcccgtccg gcagcctgac ctgcgccgtc gtcctcggct ctgcgcgggc gatggcgcct
    13801 gcgcccttct cgatcacggc tccacctttc cggctgagtc cagtcacagg cacccgcaga
    13861 ctgcggcgat taccgcggcc ggcgcgatct gcgacaccgc gtcgcgatca aagaccggca
    13921 ccctgacgag gccggccgca taccgggggc acagccgccg ctcagccgcg cgcctgctgc
    13981 cgacgagctg gcagagcggc gacctgcact atcatatcgt catgtcctcc ccggctcccg
    14041 cctccgatcg caggacgttc ctgcttcgcg tgctgcgcct tggaggcgcc agcgcggcgg
    14101 ccgtcggact cggcgcctac ctgtcggggc gcagcaggcg cccggaggag cgcgcggcgt
    14161 cgcaggtcgc cctggaccac cgcgtcgcgg accgtccgga cctgccggag ctcgccgtcg
    14221 cccgcggcga gtcggccgag cccgcagccc tcgtccggcg cgccatcgcc gacatgggcg
    14281 ggatgggccg cttcgtggcc cgcggcgcga cggtcgtcat caagccgaac gtcgcctggg
    14341 accgcttgcc agagtacgcc gccacgacga atcctgagat cgtggcccag ctcgtccgcc
    14401 tctgccgcga agccggcgcc ggggccgtca tcgtcaccga cgtcagctgc aacgaacccg
    14461 aggcctgctt cgagcgcagc ggcatcggcg ctgccgcccg cgccgccggc gccgaggtcg
    14521 tgctgccgca gacgcggttc ttcaagacga tcgatctcgg cggacaggtg ctccgcgagt
    14581 ggccggtgct ggagcccttc ctcgaggccg acacgctgat caacgtgccc atcgccaagc
    14641 accacagcct gacgggcgtc acgctgggca tgaagaactg gtacgggctc atcggcggtg
    14701 accgcaaccg cctccaccag cgcatccacg agagcctcgt cgacctcacg gacttcgcca
    14761 agccctcgct gacggtcatg gacgcctacc gggtgctcat gcgcaacggc ccgaccggcg
    14821 gcaacgtgga cgacgtcgag gcgaagggca ccgtgctggc gagcacggac ccggtcgccc
    14881 tggacgccta cgccgccaag gcctggtggg atctcgacgt cccgaggctg cgctatctgc
    14941 gcatcgccga agcgcggggg gtgggccggc ccgccttcga gtctctccgg actgtggtct
    15001 ccaacctctg accaagaccg aggggtcggg acgaattacg tgcgcccccc cacactggac
    15061 gacatttccg tcagcgactc gcccgccgaa caaaactgtc atcgtcgaag actcgttgcg
    15121 taattagtcc cgacccctca ggtagccgcg gaactcgccc cagctgcgcg ccgcgtaccc
    15181 cagtagcacc agcggcgacg cggcgacggc ccaaagaagg taccgcgggc atcgacgcag
    15241 gcgccacagc gtcacgccga gcatcagaac cggcgtcacg ggcgatgcca gcgcgcgccc
    15301 gagccggacc gcgacagaca gctgcttctc ccgacacagc actcgcgcgt acccgcgccg
    15361 gtgctcaacg atgtggccga ggaaccggcc gagtcccgcc gggctgtgat gcacgacgcg
    15421 aatggccggc gtgaaccagg ctcggtgccc gtcggcccgg gtcttccact ggaacgccgt
    15481 gtccgatgag taggatcctt ccacgaaggg gccgtagcgc tcgaaagcct cgcgcttcat
    15541 cgacagcgag cagccgggca cctcgtccac gtagcggccg cggggcgatg gcacccagag
    15601 gttgaactcg cagaagtagt acgcccaaga cacgaggctc cgcgtcgggg cgttctcgac
    15661 ggccccgccc accagcagtt cagaccgctc gtgcgcggca cacacgagat cgacccacgc
    15721 gggtccgacc gtgcagtcgg cgtcgaggaa tgcaatgagg ggcgcgcgtg cgtgcgggat
    15781 ggccagattg cgggctgccc cggcgtgcag ccgcgtgggc gacgtgagga cggcgacctc
    15841 cggataggcc ccccggacgt aggccgccgt gccgtcgtca gagctgtcta cgagcagcgt
    15901 ctcgaacgtc cgcgaggttc gctgcccgcg gagcgagtct aggcaggcgc cgacgacctg
    15961 cctcgcgcgg taggcggtga tgacgacggc caggtccgga ttccgcacgg tcatcgtgaa
    16021 gtctctccga ggctcgaccg aatcccggga ggccggtcga ggaacagggt ctccccgatc
    16081 gtctcgcacg ctaggctgcc gccttcacac gcgtagctgc actcgccgca gaagcccatg
    16141 gaggaccagc cccgcttgaa ctcgcggcga gtccgtcggt aggccgcgcc gctccagacg
    16201 gcccagaggg tgctgtcgcg gacgcttccc agcgagtact cgtccgccgc agccagcctg
    16261 ccaccctgcc ggtactactc ataggttcgg agcgtcgtcc cgtcaggcgc gaagatgatt
    16321 gtgtcgagcc ccgaacgaac gagcgcctcg gcgtggccgg cccgcgcgaa caggaggccg
    16381 ttcgtgctcg agctggcctt gacggacttc gccttcgcgt gcgcgatcat gtcgtacacg
    16441 gccgggttca gaaacggctc ccctcaatcc tacagccgca gcgtaaacgc acagggcccc
    16501 gcctcgtcca ggaccttgcg gaacagggcc gcgtccgtca cgccctgcgg gcgcgtgaag
    16561 ccgacgttca ccgggtacag cgcgcagcgg aggctgcccg actcggcgga ggccggtccc
    16621 aagttcgggc acctcgtccg ggcgccgggc gcgttcaccg gcggaccggc tcatcggctt
    16681 catcggcgag cacggcagcg cgtcgcatcc ggcctccttc gcgaccccga ccatgcggtc
    16741 gagcgcgcgg aggccctcgc ggtcgatcgt ccgacgcagc aggagccggg gccgcctcgg
    16801 agggccaccc ggccgcccgt ccgacacggc gcgaacgccg gccacggctc ggccgaacag
    16861 gaccggctgc gttccgccgc ggttctggcc gcactctcgc tcaccgggcg cccaaaggct
    16921 gacgcgcagt ccgtttagcc ccgaggccgc gacggcggtg gcggcgcggc cgtcgagccg
    16981 cgcggcgttc gtcagcagcg tgagccggaa cccggcgcgc atgaccaccg agatcatgtc
    17041 gaggatacgc aggtgcagcg gggattcgcc ctcgccgatg aggctgatct tgctcgtccc
    17101 cctcgcacga agctcgcggc aaatccgtgc caagccgctc ctcgcgaggc cgtcgcgctg
    17161 cccggccggc ttccaggcgc ttccccatga gtgggcggcg cagccgaagc agtggaggtt
    17221 gcagcggcgg ctcacggcca cgacgccgga gacggggccc ttcagcgccc gcgttcctgc
    17281 cgcgtggctc gagacaagct gcgctcgcca cagccagccc cggttcggca cccgcctact
    17341 ttctcacatt cggcgctcgc cgaccgaact cagtgcacga cgaaccgcct ggagcggccc
    17401 ggtccggtgc tcgagtctgc ctccgtctgg tcggggtact catcaagcgc ggcagccttt
    17461 tcaggatccg gggagtcctg cccgatggct cccacgccat cgccgagtgc gccgccgcct
    17521 cccgccctgc cacggggcag cccgtgcctc gccgccagcg cttcctggac catcgagacc
    17581 gcgcgcgccc gcatcacacc gacgacatgg agcgtcagca ggaaccaccg caccgtcgtg
    17641 cccgccaccg gcacgtcgta catggcgagc acctcaccct cgtcgtcgcg gagcagccgg
    17701 aggaacgggc aagcgcgggc gatcgccgcc agttgcgcat ccgatacgcc cagaccgccc
    17761 gggatgggcg tgatgaaccg cattgcctcg tagcgaaagg gctccttgcc cacctcgccg
    17821 ttcaactcga ccgtcaggcg aaacggctcg cccgcgtgaa ccagccgccg cctcaccccg
    17881 ggactcaccc gtcccgccgg cctagcatcg atgagacgga cgccgcccat ccggagcatc
    17941 cgctcgacgt catcgcgccc gagcgccggg aagaccgggg catccacggt gcggcgtccg
    18001 gcgcggtagg ccagttcgag ggcgctcgcc gctcgatagc cgaagacgtc gacgaggatc
    18061 gtgacgagtt ccgccgccag cgcacgcgcg tccgcgagcg gcgaaagcgg ccaccgcttc
    18121 ccgaagttcc gggcccggcc tccgactctg tagccgagcc gccggagggc ggcccgctgc
    18181 ggcgggccga tgtacggacg gctggcggaa ttccaggcgc ccgagcaggc ctcgacgaga
    18241 acgggctcct cgggctccgt ccagaattgg acgtagaact cgacattgcc tttcagcacg
    18301 aacgacagca ccaggtaacg gtagtcgccg acctcccagc cgctccccag ggagccccac
    18361 tcgcacgccc gcgagaccac ggccgccacc cgatcctccg cgagcgcccg agcggcgtcg
    18421 gccgacagcc ggcggccgcg agtcggcgga accagattgc accgcacttc ttcgaccgac
    18481 tgccgcggac agcagcgcgc ggcgagcgcg tcgaggctct cgcttgtccc tctcttctga
    18541 gttgccacgt cttcgcctcc cttcgccacc gccattgttg cgttgaccca tgacaggtcg
    18601 ggtcacagcc gccgggccct tgcgggcggc ccggcgggcg ccgacgaccc gagacgggcg
    18661 gcctgcttgg ccgatgcgcc ttgcgcggcc tcatagcgga gttgtagagt gccggagacg
    18721 ccaacaggag gcctccgtgt accagaccat cgcagaattc gtggacgtgt ggcggagcga
    18781 gagcgagaac acgcacaagg tcatgtgcga gctcaccgac gcgtcgctcg cacagcgggt
    18841 cgcaccggag gggcggacgc tcggccgcat cgcctggcac atcgtccaga cgctcggcga
    18901 gatgggcggc cgcgccggcc tctcagtcga aggcgcggac gagaagacgc cgcagcccgc
    18961 gacggcagca gccatcgccg atgcctaccg ggcgggagcg tcggcgctcg agaaagcgct
    19021 gcccgcggcg tggtccgacg ccgatctcgc cggcgaaatc gagttgtacg gggagaagtg
    19081 gacgcgcgcc aagaccctgc acgtgctgct ggtgcacgag atccaccacc gcgcccagat
    19141 gaccgtgctg atgaggcaag ccgggctccg cgtgccgggc gtgtacggcc ctgcgcgcga
    19201 ggaatggacg gcgtacggca tgccggccca ggagtaggcg tgcccttctg cgaccgggcc
    19261 cgccgggccg cagccgccgt tggggccgcg ctcacgctcc tgccggcggc gtcagcgccg
    19321 gccgtgggtg ccggctccgc cgcgggctgc gagtccgtcc gcgcgcagga agccttctct
    19381 ggccgcgtcg tcggcatcct cgacggcgat tcgctggtcg tgctccggga cgggaggcag
    19441 gtcaccgtgc gcctgcacgg catcgacgcg cccgagagcg gccaggccta cggcaacgtc
    19501 gcgcgacgtg cgctcgccaa ccttgcgttc ggcaagacgg tcgaggtcgc ggtgcgcgac
    19561 gtcgatcggt acaaacgcct cgtgtcccgg gtcacggccg aaggcgcgga tgtcggcctc
    19621 gagatggtgc gcgcgggctt tgcctggcac tacgcgcgct atctgcgcga cgaggcctat
    19681 gcggccgccg agcgggaggc gcgcgcagcg cggcgggggc tgtggctgga ccggagtccc
    19741 gtcgcgccat gggagtggcg cgcgaattcg aaacacgtgc gcccccgatc cggctcccgc
    19801 cggcggaact aggacgggag ttccccgccg agggcgtagt cggccgccgc ctcggcgtga
    19861 atcgacgcgg tgtcccagag cggcaccgcc gcgtcctccg gccgcagcag cagcccgatc
    19921 tcggtgcagc cgaggatgac gccctcggcc ccgcggccca cgagctcgtc gacgacttgt
    19981 ctgaacccct cgcgcgattc cgggcggagg cggccgatgc agagctcacc gtagatgacg
    20041 ccgtgcacga gggcgcgcgc ctcgtccggc ggcgtcagga cgctgagcgc gtgccgagcc
    20101 tccaggcggc cgcggtagaa gtcctgctcc atggtgaacc tggtgccgag cagcccgacc
    20161 cggcgcacgc cggccgcctt cacccgcgcg gcggtcgcat cggcgatgtg gagcagcggc
    20221 acgtccactg cggcctcgat ctgcggcgcc accttgtgca tcgtattggt gcacagcacg
    20281 atgaagtccg caccggcgcg ccggaccgct cgggcggccc ccgccaggac cttgccggag
    20341 tcttcccagc gcccttcccg ctgcatggcc tcgatctcgg cgaactcgac gctgtagagg
    20401 acgatcttcg ccgaatgcag cccgccgaga cgcgcgccca ccacgcggtt gatgatctgg
    20461 tagtacggaa ccgttgactc ccagctcatt ccgccgatca gcccgatggt cctcatacgc
    20521 accgcctccg cgaggctcac tctaccgcat ccgggttgtt ggccgtctgc tcgggatggc
    20581 gatcgggccg gcgcccctgt catttcgggg ccggccgggt gataatcggc ccgccccttc
    20641 accctggagg ctcgggagca atggcgatgt cctgccgcgg cgcgtgtggc ggcccgctgg
    20701 ccgtggtggc ttgtctggtg gccctgctgc cgggaacatc agctgcgcag acgcgcggcc
    20761 agcccgagcg ccgggtctcg gatccaggcg tcgtgacgac tcgccaagcg atcacgccgg
    20821 cgggcgcgca gagcattttc gagggccgcg tctacggcgt cgccttcggc accacgtcgg
    20881 atacgctctg ggtgctgaca agcgacgacc tgcacgagct tgactgggcc gccaacgccg
    20941 tcaggcagcg catcgacacc gggctcacgc cgggtccgca gagtctctgc ttcgaccgtg
    21001 gcggcgctca agccctcgtc gcgggcgcga gcggcatccg aacgacgggc aagcctcccg
    21061 ccgtcgtcct tggcgcggcg ctgcctgccg cgcggcccga gccgggtcag cgcgacgtga
    21121 cgccggcctc gccacggccg gctccgccgc ccgaccgccg cagcgaggcg gcgctgcccg
    21181 cgctggcgcc gatcgctgac ggcctgggct ggcacatcgc aggagccgtg tccgccgcgc
    21241 cgcgggcgcg ccctgacggc cggcggctcg cgctcgtgcc cttgatctac gacaacgccg
    21301 tcgctgtgat cgacgtcgaa ggcagcgcga tcctcggctc cgtcaggacc gaggtcgcgc
    21361 cgtttgggac ggtcgtcgac gacacgggga cggtggcgtg ggcctcgaac tggggcggga
    21421 gggttcccga ggcgggcgag cggtcggccc cgatcggcga ctacgagggc gccgaccgca
    21481 tcctggtgga tgagcgggga gtcgccgccc tggggacaat cgtccgcatc gacgtgccgt
    21541 cgctgcaggt caccgacacg attgaagtgg gcccgcatcc gacggcgctg gcatgggacc
    21601 aggcgcggga gcggctctac gtggcgctcg gcaacgacga tgccgtggag gtgatcgaca
    21661 cgtcgcgccg gcgcaaggcg ggccgcgtcg agatcagccc gttcgggcgc gggattcgcg
    21721 gcacggcgcc gaccgccgtc gcgctcgcgg ccgacggcag ccgcctgtac gtggcctgcg
    21781 gcggcatcaa cgccgtggcc gtgatcgaca ccgccgcgct gtccgtcctc ggcctgattc
    21841 ccaccgcctg gtacccggac tcgcttgcgc tcagtccgga tggcaggatg ctggccgtcg
    21901 gcgcgctgct cggcgtcgga tcgggctggc ggcgcccggc gacgccgtca ctgacgctgg
    21961 gcatgccggc ccccacgcgt cggtacgtcc atgcgacccg cggctcggtc agcgtgttgc
    22021 cggtgccgac cgacggcgag ctcgtgaact acactgccgc cgtcgccgag aacaaccgcc
    22081 tgccgctcgc cgcccgcgag tccgcgccgc ctgcgcggac ggcccgcgac gcgcggggcc
    22141 gccagccgcg cccggcgccg acggccgtcc ctctcaccgc cggggacccc tccctgatcg
    22201 aacacgtcgt ctacatcatc aaggagaacc gcacctacga ccagatgttc ggagacctgc
    22261 cgaatggcaa cggcgatccg gcgctggttc tcttcggcga ggacatcgct ccgaaccagc
    22321 gccggctggc ccgccagttc gtgctcctcg acaacttctt cgccacgggc ggcaacagcg
    22381 gcgacggcca ccagtgggtg acccaggcgg atgaatccga ctacgtcatc tggcccggct
    22441 acctcggccg gagctacccc aaggacggtt ccgacgcgct ggctcccgcg cagggcgggt
    22501 tcatctggga cgccgcccgg aagatgcgga agtcggtccg cgtcttcggc gagtacctgc
    22561 cggccgagcg gcgtgtggcg gcgtccgcgc gcgccgacct gctgcgggag tgggccaaag
    22621 gggccgactt cacgaaccgc tggaaaccga ccccgcacgt gcagggcctg aagggcatta
    22681 cggcttcgga cttcccgtcc tacacgctcg cgatccccga cgtgatccgc gccgatctct
    22741 tcctgcgcga cctccgcgcg tggcaacgca gcggcaggat gccgaacctc gtgatcatgc
    22801 tcctgccctc caatcacacg tacggcacat ctcccggcct ctccacgccg caggccatgg
    22861 tcgccgacaa cgacctcgcc ctcggccgcg tggtcgaagg gctgacgcgc agccgcttct
    22921 ggccgcgcat ggcgattttc gtggtcgagg acgatgccca ggacggggtg gatcacgtgg
    22981 acgggcaccg gaccgtcgcg caggtcatca gcccgtattc gcgccgcggc cacgtggact
    23041 cgaccttcta cgcgcagccg agcatcctga agaccatcga gctcatcctc gggctccagc
    23101 cgctgtcgat cttcgatcgc atcgccaacg acatgcgggc gagcttcact gaccaggccg
    23161 acacgacgcc gtacacggcg gtcgtgccca agcagccgct gcacgaggtg aatccgccgg
    23221 cgagcgcgtt gacgggcgcg gcgcgggagg cggcgctcgc atcggcgcgc atgcgcttcg
    23281 atgagccgga cgcggcgccg accgacagac tgaaccgctg catctggcac gccatcaagg
    23341 gatgggacgt gccgtatccg ggtgtcaggc gggcggtgtt cgcgccgctg tcggtggacc
    23401 tggatgacga tgagcgccag gacgccaggc cggacggccg cgaccgccgc tgaggggcgg
    23461 gtccctgcga cgccctgaag gcggccaggc cctgtgggcc tttgaccggg agatgagcca
    23521 ggccttctgc ccggcacgag ccgcgatcgg ttcttccctg accggccccg gagaacgtga
    23581 gatgacagcc agcacgctcc gtcacgcagt cctcaccttc gcgctggccc tgccagccgc
    23641 gctggtgctc ctggcggttt cgctcgcagc gcaggaccgc gaccgcccga tgcgggtgcc
    23701 gcaggaccca ggcgtcatca ctacgcgtca ggcgatcacc cctgccggcg tgcagagcgt
    23761 gttcgaaggg cgcgtttacg gcgtcgcctt cggcgccagc cccaatagac tctgggtttt
    23821 gaccgcgagc gatgtgtacg aactcgattg ggcgacgaat accgtccggc agcgcgtcga
    23881 caccggcttg aacgccggcc tgcagggtct gcgtttcgat cgcgcggcgg cgcgtgcgct
    23941 cgtggcgggg gccagccgtg cgagggcggc cggcaagccg ccggaggtgc ggctcgaggc
    24001 ggccgcagct tcccttgatc gcgtgccggc ggtttcggat ggcgcggcag cgcccgcccc
    24061 gccagtcgct ccagcaaccg ctccggcacc cggcgccacg gcgccgatca cgccagtcac
    24121 gccgatgggc cgcgtcgaat tggcctctcc cctctcgctg gaactggtcg ccggtgggct
    24181 tgggtcacac atcgccgggg ccctcgcggt ggcgccgcgc ccccgcgccg acggccgccg
    24241 tgtcgccctg gttccgcttg tcgcgagcaa ccaggtgtcc gtcgtggacg tcgggagcgg
    24301 cgccgtcctc ggcgccgtca agaccgacat cgccccgttc gccgccgtgg tgaacgacga
    24361 cggtaccacg gcatgggtat cgtgctgggg cggccgggtg ccgctcgaag gcgagctggc
    24421 cgcgacgacc ggcgagaacg ccggcgctga ccgcgtggtg gtggacgcgc gcggcgtcgc
    24481 ggcttcaggc accgtcgcgc gaatcgacct cgacgccatg cgcgtcacgg gcacgctcga
    24541 agtgggcctc cacccgacgg cgctcgcctg ggacgaggcg cgcgcgaggc tctacgtcgc
    24601 gctgggcaac gacgatgcgg tggcggtagt cgacaccgaa cagcgtgtgc tcgccggccg
    24661 gatcgaggtt cggccgttcg ggcgggacgt caggggcatc gcgccgaccg cgcttgcgct
    24721 cagcgcagac ggcggccgct tgtacgcggc ctgcgggggc atcaacgcaa tcgccgtgat
    24781 cgagacggca gggcgttcgc tggccggact cattcccaca gcctggtatc cggattccct
    24841 cgcgctcagc ccggacggag caatgctggc cgtcggcgcg ctgctcggcg ccggctccgg
    24901 ctggcggcgt ccagccacgc cttcgttctc gctgggcctg ccggacacgt cgcggcgcta
    24961 cgtgcacgcg cacc
//
LOCUS       DAOSIF010000004        24351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_2301_length_24351_cov_4.351622, whole genome shotgun sequence.
ACCESSION   DAOSIF010000004 DAOSIF010000000
VERSION     DAOSIF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 24351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24351
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_24351_cov_4.351622"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PLT35_00335"
     CDS             <1..679
                     /locus_tag="PLT35_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27698.1"
                     /translation="LEEDARLTALFLWTLQATASDEDESPRARRRDDEDDKEGEDDED
                     DAPRKKARKGLTLIFDVVRRFAQPLGIHLDQFEDRIISTEKGIVRLLPVSERAEQLFG
                     REGASAVAGRIEAGTKGGRQLSLDFIFDRDADERRTSRGGRRAASHVRDEDLTGDRGG
                     TTLDRLHVGMLLQSAGRANALRAFLKAEVERSPDFMRLANALSALYPKDSEEKRLLDA
                     MLLAAPR"
     gene            797..3484
                     /locus_tag="PLT35_00340"
     CDS             797..3484
                     /locus_tag="PLT35_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOG27699.1"
                     /translation="MAGTAPKDYRDPALFVSRTSFTKALREHLRLVLRRLAGETSDSA
                     PVTTLITQFGGGKTQTLTLLYHLARHGAKVASDPAVASLLGEAGIAEPPKTRIAVFVG
                     SAWDPQPGRETPWIDVARQLAGERGVELLGKDRLTAPPGTTAIAEIIAAAGGSALFLF
                     DEVLNCLNRHRALADPFHSFIQNLTVAVTGSPRSAAVVSLPRSEVEMTDWDRAWQEKI
                     TKVVRRVAKDLIANDESQISDVIRKRLFEDVGKPAVRRGVAEAYAEWCYQRRAELPPE
                     WTAVDTTATEAHARDLLRQRFEACYPFHPATLTVFQRKWQTLPQFQQTRGTLAMLAQW
                     VSLAMREGYQLARKEAVITLGSAPLGDRGLRSAVLGQLGDSRLGAAIAADISTSNSHA
                     AALDADATGALRDIHRRVGTAVLFESSGGQGDKAAHLPELRFALGEPGMDSTSIDNGL
                     VALERKAYYLRKVGADGYRFGSKPKLNQVKSNRLASLDEGEVKRAMAALVEKEFEVNQ
                     RLPVIFLKADGSEVPETTRLTAIVLPPTEELDEKGALRLRVSEWTRTKGTSPRLYPGA
                     LLWVVRKPGAALRDSVADWLAWRRVQRELQEGRLGHDFDKEERSSIGADVRDAEDKAR
                     EEVWASYRYVLLADRDQPDGVGVLDLHAGHAADTGSLAGRIINALKTETLLNDSIGAS
                     YLDRKWPPALAESGAWPLVSLRQAFLNCGLDRVPDADAVLRAKIPEFVKKGEFGLASG
                     ARPDGSYSRVWFGDKDELDPDEIVFESDIYLLKKARAKRLRGVPAAGTTSGELPRPAG
                     SPGGVEPEEIPPETMRATGQARPGGDVVIELRGTIPPDIWNRLGTRLVPKLRSGQHLH
                     IEVRAEVTANAGEVEPLVAEIRQVLTDLQLSGQVDVRVR"
     gene            complement(3549..3902)
                     /locus_tag="PLT35_00345"
     CDS             complement(3549..3902)
                     /locus_tag="PLT35_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27700.1"
                     /translation="MLCAARGAAVAGGALGIGIALVTPTIVGALSVFYAMLGATLFVP
                     VLVALHARTDRRTEVMASILAGAAAVTAVQLGVGTRPGSVLTPATAGLLTAAITYGSA
                     VALRAARHAPGERRR"
     gene            complement(3903..4151)
                     /locus_tag="PLT35_00350"
     CDS             complement(3903..4151)
                     /locus_tag="PLT35_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27701.1"
                     /translation="MSLHAGLLVAYSAVLLALGLAVSRRVRSASGFFVADRRLGGVLL
                     AAIFSAEVSTSDPVLFMLATSLSQDLYRRFVNPAASDA"
     gene            complement(4153..5142)
                     /locus_tag="PLT35_00355"
     CDS             complement(4153..5142)
                     /locus_tag="PLT35_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOG27702.1"
                     /translation="MTETRGAGLTAVAGITVGHCTLAERPTGCTVVLAGSGAVAGVDV
                     RGAAPGTRETDLLNPLNLVDKVHGIVLAGGSAFGLDAASGVVRYLEERGIGFPTDYGV
                     VPIVPAAILFDLGVGDPKVRPGADCGYRAAAAASAGPVPEGSVGAGAGATVGKLLGMA
                     RAMKGGVGSAALTLPGGLTVAALVAVNAFGDVIDPATGQVVAGVRTADGRGLADARLR
                     MRAGTALKPPLGTSTTIGVVATNARLTKTQATKVAQMAHDGLARALAPVHTLADGDTL
                     FALATGALAADADLSTVGALAADAVSDAVVRAAREAKGLPGLPAAGDLGPARR"
     gene            complement(5387..6145)
                     /locus_tag="PLT35_00360"
     CDS             complement(5387..6145)
                     /locus_tag="PLT35_00360"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOG27703.1"
                     /translation="MIRLPRFPPLPPAARIAACAACLTILPAAASAQFPVGRLFGSVR
                     DIAGRPIGGASVHAVNPSAVPSAFSATVNKKGEWSILGPRSGVWKVSAEAPGFEPATV
                     SVPVSLAQRAEPIDFVLVALPARSPLDGVDTKRLQDDLSRASALMAGARWDEAVAAYR
                     AILARVPLLDSVNFAIGRALRMKKDYAGAEAAYRALLERDARNQQALLEVGRTQIEAG
                     NGAAAAATLDGLLAIDGSTEEAAEARELLARIRK"
     gene            6207..8645
                     /locus_tag="PLT35_00365"
     CDS             6207..8645
                     /locus_tag="PLT35_00365"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG27704.1"
                     /translation="MRRGFLSLVLALATASLAAARPLPAPPPRPAGQGAPLRGAAADA
                     SRLAAVDAVIEQAIRDKQIPGAVLLVGRGQDVLYEKAYGNRALVPRIEPMTLDTIFDL
                     ASLTKVVATTTSVMILVEEGRIRLTDRVASFIPDFGRYGKEGVTIRHLLTHVSGLRPD
                     LELTDDSWRGSDEAIRQACEEVLVAPPGERFVYSDINFFLLSEIVARVSGTPFDRFVA
                     DRVARPLGMTDTMFLPPASLTARVAPTELCTPYGWPCEGPNREMLRGTVHDPTARRMG
                     GVAGHAGLFSTAADLSRYCRMVLGGGALGGARVLSPLTVLKMTSPATPPGMASVRALG
                     WDIDTSYSSNRGELLPIGSFGHTGFTGTSLWMDPVTKTYVIFLSNRVHPDGKGDATPV
                     RARVATIVASALRLLPNDSVLRAERWTGGDFGASGTAPRRPDRAAGRTLNGIDVLRAE
                     GFARLKGKTVGLVTNHTGRARTGEPTIDLLASAKDVTLAALFSPEHGIRGILDEEVPS
                     SRDEKTGLPIHSLYGATRRPTDEMLRGIDTLVVDLQDIGSRFYTYMSTMGYVMEEAAK
                     RRIAVVVLDRPNPVTGSAIEGPTVDQGELSFTAYHPMPIRHGMTLGELALLFNAERKI
                     GADLTVVEMKYWDRDAWFDDTGLPWVNPSPNMRNMVAASLYTGVGAIEASNVSVGRGT
                     DTPFEQVGAPWIDAPRLAAELNGRDLPGVRFYPVDFTPSSSRFAGELCHGVFVIVTDR
                     ARLKPVRVGLEIASALHRLFPAAFELERVGRLFGADAAARIRAGEDPAAIAASWGRAE
                     AAWRLLRAKYLRYR"
     gene            complement(8698..10098)
                     /locus_tag="PLT35_00370"
     CDS             complement(8698..10098)
                     /locus_tag="PLT35_00370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG27705.1"
                     /translation="MPTRPLLVIALVLLLLGAAAAFYFAGQEPGPLVRIEKPAAIGGG
                     SVELDLIVDAIGGGLTRLDAAVEQEGRTLPLFSLASSGSATFTQETADRMRVKQTTPA
                     GSLAGLRDGPARVVVTAARSVLFGLRTAESVTARDVAVRMTPPALAVVSTHHYVNLGG
                     SEMVVYRVTPSDASSGVQVGDRFYPGFAAAGAAAAGARPADPSLRVAFFALLHDQDVT
                     TPIRLVATDPAGNAAGAALDRRAFPSGFRRRRLEITDRFLLQVVPGILAATPGLQASA
                     ESPEARLDAYLKINRELRQANDAQIESISRGTAPERLWSGPFLRMGRAQAEAAFADHR
                     TYVYGGREVDQQVHLGTDLASTRGAPIVAANTGRVAFAAYLGIYGNCVILDHGMGVQT
                     LYGHLSSITVEVGDRVGRGEPIGRSGMTGLAGGDHLHFAVLVAGRPVNPVEWWDPHWI
                     ADRIDRKLAASGLSAR"
     gene            10164..10238
                     /locus_tag="PLT35_00375"
     tRNA            10164..10238
                     /locus_tag="PLT35_00375"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10197..10199,aa:Glu,seq:ctc)
     gene            10341..12338
                     /locus_tag="PLT35_00380"
     CDS             10341..12338
                     /locus_tag="PLT35_00380"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG27706.1"
                     /translation="MRRSLCATLAIGCALGAAAAALAWALGQTAFLRTVELKTYDYRV
                     RLAADPASARRDLVLVSIDDSSIRRLEPQVGRWPWPRLVHASLIDYLRRAPAKAIVYD
                     VLFTERDRRTFQAGGDTWTGEESDLALATSASRAGNVVFAADVTADEVALEDVRGLSA
                     LRGSRFFEADPAFESRPGVILPYAELTEAAAAVGHSLTIYDADGPVRRTAPFVRVGTV
                     GVPSIAVAAVMVAGPVARESIRRNGDTLEVGRAAAPLVAARIPSFYGPAADARRLLIR
                     FTGPVLKDGRPTYADYSFYDLFYSEQQLLAEQAPAVDPAVFRDKIVVVGTTAAGLRDL
                     FTVPFAEGSMPGMQVHANVIDNLLSGRFMRPLGRAGNLGVLLACALAVGIAGALTAVG
                     PTLAAAALMIAGLAWASYLLIGQGVWIGVAEPVLGATFAAFGGTAYHYFAEGREKRRV
                     KKLFSRFVSRDVYEQLLADPAGARIGGARREMSVLFADIRGFTALTERGQPEAVVAQL
                     NEYFSAMVDVVFRHRGTIDKFVGDMVMALFGAPVPDADHADNAVKAALGMLAALGGLN
                     AKWAAEGRPALDIGIGVNSGDMLAGNIGSDTIMSYTVIGDAVNLGARLESLNKEYGTR
                     LIVSDATRTRLKGRYDLRPLGSVTVKGKSEEVAIFAVLPPS"
     gene            12357..13529
                     /locus_tag="PLT35_00385"
     CDS             12357..13529
                     /locus_tag="PLT35_00385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG27707.1"
                     /translation="MRAVHAPQRLQSARSRRLRLLRPLAAPLAAWALVVPGAALAAPA
                     ATQLIKIPKKPKLDTVVKRVEQLRELQVTDEEERQLGAGVSERVRERYGVVQDAAVHR
                     YVTLVGTLLAQKSDRPRLHFTFVVLDTDGVNAFAAPGGYIHVTRGALGLMSSEAELAG
                     VLAHEIVHVTEKHTIRAIQKNKAIQMTAEESIKSNKALWNKLVDVTSEMVLAGFGRGE
                     ELEADEKGLLVANAAGYAPAGLGGFLKRLEARNRTSSGRQGLFASHPEMKERLDRIER
                     EIGSRKLASAAVLGDRYRAAIPFTAAAAADIAAVEDGAAGLAGGGKPKKKGFGLGSLV
                     KPGGAEKRSAEVVGSGASRGVDTERDAKGGPVKTLVSVTITPAELEAFRAQGNLLS"
     gene            13526..14986
                     /locus_tag="PLT35_00390"
     CDS             13526..14986
                     /locus_tag="PLT35_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOG27708.1"
                     /translation="MSAPALRIAIGAVILAATLVVRAASVNRLVRRKLRLTLLLALVY
                     TAAVVVLGRYGLSAAMDVRVLSVAQLCLALALINLAVVTAFNPLRADRVPERFPAIVQ
                     DTIVIGLFLLVGTVVMREKFLTTTAVSAVVIGFALQDTLGNMFAGLAIQVEKPFRVGH
                     WVAVGPHVGLVAEVTWRATKLKTKAGNLVILPNAFMAKEAITNYSEPSALTRLDVEVG
                     VSYGSPPNQVKQALLEAIPNAPLALKDPPPDVIVADFGASAVIYRVRFWTNDFSRDSL
                     ARDQVRTAVYYALRRHGLEIPFPIQTEIHREPESEPESARNGRVNEALRQVDLFQPLS
                     IDERYALAARAGERLFGQGETIVRQGETGGSMYVVRRGRVRVIEASGRELAVFSAGGY
                     FGEMSMLTGQPRTASVQAVEDSDVVELTAESLREVALVNPDVLRRISDVVAARQADLE
                     RQKAEGAPGRPVAAEASRSLLSRIQAFLRLPALIGD"
     gene            15037..16911
                     /locus_tag="PLT35_00395"
     CDS             15037..16911
                     /locus_tag="PLT35_00395"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG27709.1"
                     /translation="MSISRQPAAIALMILMSAAAAPWAEPDPQARGRLPPPAFADPGR
                     VTRLERAFPEIDRLFKTFAERARVPGIAYGIVIDGRLAHAGAAGVRDVRTKDPVDLDT
                     VFRIASMTKSFTALCVMRLRDEGKLGLDDAADRYVPELAGLAPPTADSPRVTIRHLLS
                     HAAGFPEDNPWGDRQLAATDDGMARMLERGIPFSTATGTAYEYSNYGFAILGRVVSRA
                     AGMPYEAYVADAILKPLGMSATTLEAASVPPPRLARGYRLEEGRWVDEPLLPHGAFGA
                     MGGMLTSARDLAAYVAFLSSAWPPRDDPDTGPVRRASLREMQQVWRPSPSVARRSSVD
                     GPLQLSAGGYGFGLRVWQSCGFGHLVAHSGGLPGFGSHMRWLPEYGVALFAMGNLTYT
                     NWGLAADDAIEALARTGGLQPRVAQPSVALLDARARVIRLIDRWDEREAAAVAADNLF
                     LDEPADRRRAQLESIRAAQGACRANLGFNVENALRGTWTETCDRGWLRVGITLAPTEP
                     PLVQEWVVVPVRPLAPGLDAAAAAIARAVSAQRRGPLSIKLGAGVAPEEARRLLDSAA
                     AWGACRVGEVLASDGERAATIRLACAKGDLDLSLALDPATRVLTRLALAPAAGGTCVP
                     "
     gene            complement(16928..17425)
                     /locus_tag="PLT35_00400"
     CDS             complement(16928..17425)
                     /locus_tag="PLT35_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27710.1"
                     /translation="MAKKTPWPVIVLGIGAFALIVGAGLIAVAGYFVYQQFAIQATAA
                     TEEDAAQEFALAASRFQGQKPYLAIKDGEPIVSNDRPAKPGEPIDAIHILVWDPDQGR
                     VVRFNVPLWLLRMSKGHPIKIQHDGDEAPVRLKLTAADLEKRGPGLILDHKDAGGQRI
                     LVWAQ"
     gene            17653..18675
                     /locus_tag="PLT35_00405"
     CDS             17653..18675
                     /locus_tag="PLT35_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HOG27711.1"
                     /translation="MSRRPAPRRYRTIDAHAAGEPLRLIVEGYSAPAGGTMLEKRAWA
                     ARHADHVRRALMLEPRGHADMYGAVLTEPVTGASDAGVLFMHNEGYSTMCGHGIIAAT
                     TIALERGLIHPRRAGEIVFDTPAGPVRARAAAEARGDGAVRVAHVAFDNVPSFVLAPG
                     LELKARGRSLRADIAFGGAFYAIVDAESAGVPITGSRLADLRSLGMEIARAAEAAVPV
                     VHPLEKGLKGIYGTIFTGPPGREDADLRNVTIFADAEVDRSPCGTGTCAVMAVLADMG
                     LLPEDRAFGHESVIGTLFRGRVAGRTRVGGFDAILPEIEGSAWITGEHVFLIDDDDPL
                     RAGFRL"
     gene            complement(18688..19344)
                     /locus_tag="PLT35_00410"
     CDS             complement(18688..19344)
                     /locus_tag="PLT35_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27712.1"
                     /translation="MIVCLAGVVALAAAGCGGKKEAQQQAAGAAREPATQDQQSATEE
                     RGGGEADLVKGMQEFARAMESMQKSPDGKAYEPVSFRSLIELLPDVPGWEKEEPEGES
                     TTAPVRFSQASAGYTKGDARIEVKIVDTAMSRLLTMPYQMFLAAGYSKESTSGYEKAV
                     KVKGQPGWEKWNSESKTAELGVIVGERFLVTVEGSGADIKDAQAVAGRLDMAKLAALK
                     "
     gene            complement(19520..19843)
                     /gene="cyaY"
                     /locus_tag="PLT35_00415"
     CDS             complement(19520..19843)
                     /gene="cyaY"
                     /locus_tag="PLT35_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HOG27713.1"
                     /translation="MPDGVTDHEFAAKAESAIEALQRGLLGLADEEGFEVDLEGGVLK
                     IEFEEPSNATFVISPNAPVRQIWVSALVQSYKLAWSQETGAFALGGETLAQLVERLVR
                     LHLGR"
     gene            complement(20205..21164)
                     /locus_tag="PLT35_00420"
     CDS             complement(20205..21164)
                     /locus_tag="PLT35_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG27714.1"
                     /translation="MSRTRRFLAAAAAAALAAAAAGRGPEAAGAAAPRPEARQGGVFR
                     SGARMVPIYATVTDARGALVPDLTRAEFEVLDNGVRQDLLVFENQTQPFTAVVMLDSS
                     GSMTSSLKLVKAAAEQFLIRLLPQDKAQVGAFNDKIQFSGEFTSDRDALIAALGELDF
                     GNPTRLYDALDESLDRLAPIEGRRVIVVLTDGEDTASRRGRGDVLARAVAGEVMIYGI
                     GLESDYFNGIRRVRTSPDRVVRRLAEETGGGYFLLRETSNLSSTFTRIAQELHSQYVL
                     GFTPAVLDGRVHQLTVNVKRPGMKARARKTYVAASEAGTGPPK"
     gene            21459..21890
                     /locus_tag="PLT35_00425"
     CDS             21459..21890
                     /locus_tag="PLT35_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG27715.1"
                     /translation="MADARPPVIVNLEWDGDQAFTAHDGVHEWVLDGRNQAGPTPVVA
                     LASSLAGCIAIDIVHILARGRFDVVALEVNLRGHRADTEPRRFVRVEMRIILDTNAPQ
                     EHIDRAIALSREKYCSVWHSMRQDIELETTVVRARPRSEAS"
     gene            22022..22915
                     /locus_tag="PLT35_00430"
     CDS             22022..22915
                     /locus_tag="PLT35_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOG27716.1"
                     /translation="MKPQDLVAPFLVTDGGRFSLKDHPTGSGSWKQSKKDTRKLLEQG
                     VERLAALQERLYAQDTWGVLLIFQGMDAAGKDSVIAHVMSGVNPQGCQVCSFKTPSAE
                     ELDHDFMWRTTKALPERGRIGIFNRSYYEETLVVRVHPEILDRAKLPAAARTKTIWRD
                     RFEDINAFERYLARNGYLIRKFFLHVSKEEQKERFLERIARPEKNWKFSAADVKERER
                     WDDYMAAYEDTIRHTATLHAPWIVVPSDSKRFARIVVASAIVEALEGLDLAFPTLSPA
                     RKRELRACRAALEREGAPPRA"
     gene            23065..24198
                     /locus_tag="PLT35_00435"
     CDS             23065..24198
                     /locus_tag="PLT35_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HOG27717.1"
                     /translation="MINVSAIQMQQFGVRFYQASLTARDIDRLVRFEVLSYGQPHQPP
                     VRGKKRGAVASKVNWDLLERRIAASEQAYQRQIIRRKIDELVGYYEQCRQARDLPSIP
                     GAVIISSDEKLGFSPVEEGSSLGVLKVPEREGILRAIDGQHRLLAMHADIDRFGEEAF
                     SVPAVIFDQLPEDHIVQMFVTINAKHTRLNSSHLVSLSGRQLYRDANLALAHDIVRAL
                     NDRDDSPLHMDIKLLGVGHGSVAQAPLAQELKRLFQGDAFGGPRRTREFHDEATRFFV
                     NYFKQVALVFGAAWNGRKYSIKSSSALRAFLRVSPEVVRRLDEEHADRADFRAIGRVI
                     APWGHRIGDLRFETQGAWSKGSSVEALAKELRLALYYPEGAMG"
BASE COUNT         3602 a         8590 c         8725 g         3434 t
ORIGIN      
        1 cctggaggaa gacgcgaggc tcaccgccct gttcctctgg acgctccagg cgaccgcgtc
       61 ggacgaagac gagagtccgc gggcccgtcg tcgcgatgac gaggacgata aagaaggcga
      121 agacgacgaa gacgatgcgc cgcgaaagaa agcgcgcaaa ggactcacgc taatcttcga
      181 cgtcgtccgc cgcttcgctc agccgcttgg catccacctg gatcagttcg aagaccgtat
      241 catctccacg gagaagggca tcgtccggct cctgccggtt tcggagcgcg ccgagcaatt
      301 gttcgggcgc gaaggcgcat cggccgtcgc cggacggatt gaggccggca cgaagggcgg
      361 ccggcagctg tcgctggact tcatctttga ccgcgacgcc gacgagcgca gaacatcgcg
      421 agggggccgc cgagccgcgt cgcacgtccg cgacgaggat ctgaccggcg acaggggcgg
      481 gaccacgctc gaccgcctgc acgtcgggat gctgctccaa tccgccggcc gggcaaacgc
      541 cctccgggcg tttctgaagg ccgaagtcga gcgcagcccg gacttcatgc ggctcgccaa
      601 cgccctgtcc gcgctctacc cgaaggacag cgaagaaaag cgactgctcg acgccatgct
      661 gctcgcggcc cccaggtagc tcccgaccac aggaagacgc catggaaccc tggtacagag
      721 tcgtcacgcc gcgaaggaag tcagagaggg gcgatcgttc gatccaagcg agttcgccat
      781 ccacctcgag caggttgtgg cgggaacggc gccgaaggac taccgcgatc ccgcgttgtt
      841 cgtgtcacgg acgtcgttca cgaaggcgct ccgtgaacac ctgcggctgg tgctgcggcg
      901 tctcgcgggc gagacgtcgg actcggcgcc cgtgacgacg ctcatcacgc agttcggcgg
      961 cggcaagacg cagacgctga ctctgctgta ccacctcgcc aggcacggcg cgaaggtggc
     1021 gtccgacccc gctgtggcgt ccctgctggg ggaggctgga atcgccgagc ctcccaagac
     1081 cagaatcgcc gtgttcgtcg ggagcgcgtg ggatccgcag ccgggccgcg agactccctg
     1141 gatcgacgtc gcgcgccagc tcgccggaga gcgcggcgtg gagctgctcg gaaaggaccg
     1201 gctaacggcg cctcccggga cgaccgcgat tgccgagatc atcgccgccg ccggcggaag
     1261 cgccctcttc ctcttcgacg aagtcctcaa ctgcctgaac cgccacaggg cgctggccga
     1321 cccgtttcac tccttcatcc agaacctcac ggtggccgtt accggatcgc cccgctccgc
     1381 ggccgtcgtc agcctgcccc gcagcgaagt cgagatgacg gactgggacc gggcctggca
     1441 ggagaagatc accaaggtcg ttcgccgcgt cgccaaggac ctcatcgcaa acgacgagtc
     1501 gcagatcagt gacgtcatcc gcaagcggct gttcgaggat gtcgggaagc cagcggtgcg
     1561 gcgcggcgtc gccgaggcgt acgccgagtg gtgctaccaa cgccgcgcag agcttccgcc
     1621 cgaatggacg gcggtagaca cgaccgcgac cgaggcgcac gccagggatc tgctgcggca
     1681 gcgcttcgag gcgtgctacc cgttccaccc ggccacgctc accgtattcc aacgaaagtg
     1741 gcagacgctg ccgcagttcc aacagacccg aggcaccctg gcgatgctcg cccagtgggt
     1801 gtcgctcgcg atgcgcgagg gataccagtt ggcacgcaaa gaggctgtca tcaccctggg
     1861 ctcggctccc ctcggcgatc gcggccttcg atcggccgta ctgggccagt tgggagattc
     1921 gaggctcggg gcagccatcg cggccgacat cagcacgtcg aacagtcacg cggctgccct
     1981 cgacgctgac gcgaccggag cgctgcggga catccatcgc cgcgtcggca ccgccgttct
     2041 gttcgaatcg tccggcgggc agggcgacaa ggccgctcat ctgccggagc tccggttcgc
     2101 gctcggcgag cccgggatgg actcgacgtc catcgacaac gggctcgtgg cccttgaacg
     2161 aaaggcctac taccttcgta aggtcggcgc ggacgggtat cgcttcgggt cgaagccgaa
     2221 gctgaaccag gtgaagagca accggctcgc gtccctcgac gagggcgaag tgaagcgggc
     2281 catggctgcg ctggtcgaga aggagttcga ggtcaaccag cgcctgccgg tcatcttcct
     2341 gaaggcggat ggttccgagg ttcccgagac gacgcgactg accgcgatcg tgctccctcc
     2401 cacggaagag ctggacgaaa agggcgcgct gcgcctgagg gtgtcggaat ggacccgcac
     2461 gaagggaacg tcgccgaggc tctacccagg ggcgcttctg tgggtggtac gcaagccagg
     2521 agcggccctg agagacagcg tggcggactg gctagcctgg cggcgcgtcc agcgggaact
     2581 gcaggagggg cgcctaggcc acgatttcga caaggaggag cgatcgagca tcggggcgga
     2641 cgtgcgggac gccgaggaca aggctcgcga ggaggtgtgg gccagttacc ggtacgtcct
     2701 gttggcggac cgggatcagc cggacggcgt aggcgtgctc gatcttcacg cggggcatgc
     2761 ggccgacacc ggttcgcttg ccgggcggat catcaacgcc ctcaagaccg agaccttgct
     2821 gaacgattcg atcggagcga gctacctgga tcgcaagtgg ccgccggcgc tagctgaatc
     2881 gggagcctgg ccgctggtga gcctccgcca agccttcctc aactgcggcc tcgacagggt
     2941 gcccgatgct gatgccgtcc tgcgggccaa gatcccggag ttcgtcaaga agggggagtt
     3001 cggcctcgcg tccggggcac ggcccgacgg ctcctactcg cgcgtgtggt tcggcgacaa
     3061 ggacgaactc gatcccgacg agatcgtctt tgagtcggac atctacctcc tgaagaaggc
     3121 gcgggcgaag aggctgaggg gggtgccggc ggctggtaca acgtcgggcg aactgccgag
     3181 gcccgctggg agtccgggtg gtgtcgagcc cgaggagata ccgccagaga cgatgcgagc
     3241 gactgggcag gcgcgacccg gcggggatgt ggtgattgag cttcgaggaa ccatcccgcc
     3301 ggacatctgg aaccggctgg gcacgcgcct cgtgccgaag ctgcgcagcg ggcagcatct
     3361 ccacatcgag gtccgcgcgg aggtcaccgc gaacgctggc gaggtagagc cgctggtggc
     3421 cgagattcgg caggtgctga ccgacttgca gctgagcggc caggtggacg tccgcgtccg
     3481 ctgaaccctt agctcgatgt ggcgcgctgc gcacgcctgt ctcccgcggg ctccagccga
     3541 gagcgccttc agcgccggcg ttcgccgggt gcgtgtcgcg cggcgcgaag ggcgacggca
     3601 gacccgtacg tgatcgcggc agtcagcagc cccgccgtcg ccggcgtcag gacgctgccc
     3661 gggcgcgttc cgacgccaag ctgcacggcg gtcacggcgg ccgccccggc caggatcgac
     3721 gccatcactt cggttcgccg atccgtccgc gcgtgcagcg ccacgagcac cggcacgaac
     3781 agcgtcgcgc cgagcatggc gtagaagacg ctcagcgccc cgacgatggt gggcgtgacg
     3841 agcgcgatgc cgatgccgag ggcgcctccc gcgacggccg ccccccgcgc ggcgcacagc
     3901 acctacgcat cggacgcggc gggattgacg aagcggcggt agaggtcctg cgagagcgag
     3961 gtcgccagca tgaacagcac cgggtccgac gtgctcactt ccgcggagaa gatggcggcg
     4021 agaaggacgc cgccgagccg ccggtcggcc acgaaaaacc cagacgcgct ccggacacgc
     4081 cggctgacgg cgagcccaag ggccagcagg accgcggagt aggcgacaag gaggccggcg
     4141 tgcaggctca tgtcagcgcc gcgcggggcc gagatcgccc gcggcgggca ggccgggcag
     4201 gcccttcgcc tcgcgggcgg cgcgcacgac ggcgtcggag acggcgtcgg cggccagcgc
     4261 gcccacggtg ctgagatcgg cgtcggcagc gagcgcgccg gtcgccagcg cgaagagcgt
     4321 gtcgccatcg gccagcgtgt ggaccggggc gagggcgcgc gcgagcccgt cgtgcgccat
     4381 ctgcgccacc ttcgtcgcct gcgtcttcgt caggcgcgcg ttggtcgcca cgacgccaat
     4441 cgtggtgctc gtgccgaggg gcggcttgag cgccgttccc gcacgcatgc ggagcctggc
     4501 atcggccagt ccgcgcccgt cggccgtgcg gacgccggcg accacctgcc cggttgccgg
     4561 atcgatcacg tccccgaacg cgttcacggc caccagggcc gccaccgtca ggccgccggg
     4621 cagggtcagc gccgcggagc cgacgccgcc cttcatggcg cgcgccatgc cgagcagctt
     4681 ccccaccgtc gccccggcgc ccgcgcccac gctgccctcg ggcaccgggc cggccgaggc
     4741 ggcggcggcc gcgcgatagc cgcagtcggc cccgggcctc accttgggat cgccgacgcc
     4801 caggtcgaac aggatggcgg ctggcacgat gggaacgacg ccgtagtcgg tcggaaaccc
     4861 gatcccgcgc tcctcgaggt agcgcacgac acccgaggcg gcatcgaggc cgaaggcgct
     4921 gccgccggcg aggacgatcc catgcacctt gtccacgagg ttgagcggat tcagcaggtc
     4981 cgtttcacgc gtgccgggcg cggccccgcg gacgtccacg ccggcgaccg ccccggagcc
     5041 ggccagaacg acagtgcagc cggtcgggcg ttccgccagg gtgcagtgcc caaccgtgat
     5101 gcccgcaacg gccgtcaggc cagctcccct ggtttccgtc attgccgaga cgcctgccta
     5161 aagcgccaga gcggccagca cgaacgcggc ggcctggcag cgccgggcga agaacgcgcg
     5221 cctgcgcgac acggggcgga tggtagcaca gagaggcgga tgtcaggcgc gcggagccgg
     5281 gcggcagtcc gggcgggcgc gaggcccaag gggcagggcg agcggctggc gggccggcgg
     5341 ccgaaggcgg aggggccggg tcccgggctt cggagctcgc ctcgcgtcac ttccggatcc
     5401 gggccagcag ttcgcgcgcc tccgccgcct cctcggtcga accgtcgatc gcgaggaggc
     5461 cgtcgagcgt agcggcggcg gccgcgccgt ttcctgcctc gatctgggtg cgtccgacct
     5521 cgagcagcgc ctgctggttc ctggcgtcgc gctccagaag ggcgcggtag gccgcttcgg
     5581 ccccggcgta gtccttcttc atgcggagcg cgcggccgat ggcgaagttc acgctgtcca
     5641 gaagcggcac cctcgcgagg atcgcgcggt acgccgcgac cgcctcgtcc cagcgcgcgc
     5701 cggccatcag cgcggaagcc cggctcaggt cgtcctggag ccgcttcgtg tcgacgccgt
     5761 cgagcgggct ccgggcaggc agcgccacca gcacgaagtc gattggctct gcgcgctggg
     5821 ccaggctcac cggaacgctc accgttgcag gctcgaagcc gggcgcttcg gccgacacct
     5881 tccatacccc cgaccgcggc ccgaggatgg accactcgcc tttcttgttc accgtcgccg
     5941 agaacgcgga cggaacggcc gaggggttca cggcgtgaac ggaggctcct ccgatcggcc
     6001 ggcccgcgat gtcacgcacg gacccgaaca gtcggccgac gggaaactgc gcagacgcgg
     6061 ccgcggggag gatcgtcaga cacgcggcgc aggcagcgat gcgcgccgcc ggcggcagcg
     6121 gcgggaagcg ggggagccgg atcactcgaa ctccttgggg gtctgtgcta gcttaccatc
     6181 cgcggctcgt tgcccgggag gtgcgcgtgc gacgtggttt cctgtccctc gttctggccc
     6241 tggcaaccgc gtccctggcg gctgcccggc cattgccggc cccgccgccg cgtcccgccg
     6301 gacagggcgc gcccctgcgg ggcgccgcgg ccgatgcgtc ccgcctggcg gccgtcgacg
     6361 ccgtcatcga acaggccatc cgggacaagc agataccggg ggccgtgctc ctggtcggca
     6421 ggggccagga cgtgctctac gagaaagcgt acggcaaccg ggcgctcgtg cctcggatcg
     6481 agccgatgac cctcgacacg atcttcgatc tcgcgtcact caccaaggtc gtggcgacga
     6541 cgacgagcgt gatgatcctg gtcgaggagg ggcggattcg gctgacggac cgggtggcgt
     6601 cgttcatccc cgatttcggg cgctacggca aggagggcgt gaccatccgg cacctgctga
     6661 cgcacgtctc ggggctgcgg ccggacctcg agctcaccga cgactcgtgg cgcggctcgg
     6721 acgaggccat ccggcaggcc tgcgaggaag tgctcgtggc gccgccaggc gagcgcttcg
     6781 tgtacagcga cataaacttc ttcctgcttt ccgagatcgt cgcccgggtc tccggcacgc
     6841 ccttcgatcg gttcgtggcc gatcgcgtcg ccaggccgct gggcatgacg gacacgatgt
     6901 tcctgccgcc ggcgtccctg acggcgcgcg tcgcgccgac cgagctgtgc acgccctacg
     6961 ggtggccgtg cgaggggccg aaccgcgaga tgctgcgcgg cacggtgcac gatccgacgg
     7021 cgcggcggat gggcggcgtg gcgggccacg ccgggctgtt cagcaccgcc gcggacctgt
     7081 cgcgctactg ccgcatggtg ctcggcggcg gcgccctcgg cggcgcgcgg gtgctgtcgc
     7141 cgctcacggt cctcaagatg acgtcgccgg ccacgccgcc gggcatggcc agcgtcaggg
     7201 ccctcgggtg ggacatcgac accagctact cgtcgaaccg cggcgagctg ctgccgatcg
     7261 ggtccttcgg ccacacgggc ttcaccggca cgtccctctg gatggatccc gtcacgaaga
     7321 cgtacgtgat cttcctctcc aaccgcgtgc accctgacgg caagggcgac gccacgcccg
     7381 tgcgggcgcg cgtcgccacg atcgtggcgt cggcgctgcg gctcctgccg aacgactcgg
     7441 tcctgcgcgc cgagcgctgg acgggcgggg atttcggcgc gagcggcacg gcgccccgcc
     7501 ggccggatcg ggccgccggc cggacgctca acggcatcga cgtcctccgc gcggaggggt
     7561 tcgcccggct gaagggcaag acagtcggcc tggtgacgaa ccacaccggc cgcgccagga
     7621 cgggggagcc cacgatcgac ctgctcgcgt ccgcgaagga cgtgacgctg gcggcgctct
     7681 tcagccccga gcacggcatc cgaggcatcc tggacgagga ggtcccctcg tcccgggacg
     7741 agaagaccgg cctgccgatc cactcgctct acggcgccac caggcggccg accgacgaga
     7801 tgctgcgggg catcgacacg ctggtcgtgg acctgcagga catcgggtcg cgcttctaca
     7861 cgtacatgag cacgatgggc tacgtgatgg aagaagccgc gaagcgccgg atcgccgtcg
     7921 tcgtcctcga ccggccgaac ccggtgaccg gatcggccat cgagggcccg accgtcgacc
     7981 agggcgagct gagcttcacg gcgtaccacc cgatgcccat ccgccacggg atgacgctcg
     8041 gcgagctggc gctgctcttc aacgccgagc gcaagatcgg cgccgatctc accgtggtcg
     8101 agatgaagta ctgggaccgc gacgcgtggt tcgacgacac ggggctgccg tgggtgaacc
     8161 cctcgcccaa catgcggaac atggtggcgg ccagcctgta caccggcgtc ggcgcaatcg
     8221 aggcgtcgaa cgtgtcggtg gggcgcggga cggacacgcc gttcgagcag gtcggcgcgc
     8281 cgtggatcga cgctccgcgc ctggcggcgg aactgaacgg ccgcgacctg ccgggcgtgc
     8341 gtttctaccc cgtcgacttc acgccctcgt ccagccggtt cgccggcgag ctgtgccacg
     8401 gcgtgttcgt catcgtgacc gaccgcgcgc ggctcaagcc ggtccgcgtg gggctggaga
     8461 tcgcgtcggc cctgcaccgg ctgttcccgg cggcgttcga actcgagcgc gtgggccggc
     8521 tgttcggcgc cgacgcggcc gcgcggatcc gcgccggcga ggacccggcg gccatcgccg
     8581 cgtcatgggg cagggcggaa gcggcctggc gcctgctgcg cgcgaagtac ctgcggtacc
     8641 gctgacggcg atcgggcggc agcgccggtt cccgggaatc cgcggaaagc gcgcccgtca
     8701 cctggccgag aggcccgacg ccgcgagctt gcggtcgatg cggtccgcaa tccagtgcgg
     8761 gtcccaccac tcgaccggat tcaccggccg ccccgcgacc agcaccgcga agtgcaggtg
     8821 gtcgccgccg gcaaggccgg tcatgccgct ccggccgatg ggctcccccc ggcccacccg
     8881 gtctccgacc tccacggtga tactcgacaa gtggccgtac agcgtctgga cgcccatgcc
     8941 gtgatcgagg atcacgcagt tgccatagat gccaagatac gcggcgaagg cgacgcggcc
     9001 ggtgttcgcg gcgacgatcg gcgcgccgcg ggtcgaggcc aggtccgttc cgaggtgcac
     9061 ctgctggtcc acctcgcgcc cgccgtacac gtaggtccgg tggtcggcga aggccgcttc
     9121 cgcctgcgcc cggcccatgc gcaggaacgg cccgctccac agccgctccg gcgccgttcc
     9181 acgcgagatc gactcgatct gggcgtcgtt ggcctgcctc agctcgcggt tgatcttgag
     9241 atacgcgtcg agcctggcct cgggcgactc ggccgaggcc tgcaggccag gcgtcgcggc
     9301 caggatgccc gggacgacct gcagcaggaa gcggtcggtg atctcgaggc ggcggcgcct
     9361 gaacccggac ggaaacgccc gccggtcgag ggcggcgccg gccgcgttgc ccgcggggtc
     9421 cgttgcgacg aggcgaatgg gcgtcgtgac gtcctgatcg tgcagcaggg cgaagaacgc
     9481 cacgcgaagc gacgggtcgg ccgggcgggc cccggcggcg gcggcgccgg ccgcggcgaa
     9541 gcccggatag aaccgatcgc ccacctgcac gccggacgag gcgtcggacg gcgtcacgcg
     9601 gtagacgacc atttccgatc cgcccaggtt gacgtagtgg tgcgtggaca cgacggccag
     9661 cgccggcggc gtcatgcgca cggcaacgtc ccgcgccgtg accgactcgg ccgtccgcag
     9721 gccgaagagg acgctccggg ccgccgtcac cacgacgcgt gcggggccgt cgcgcagccc
     9781 cgccagcgac ccggccggcg tggtctgctt cacgcgcatc cggtccgccg tctcctgcgt
     9841 gaacgtcgct gacccggacg aggcgagcga gaacagcgga agggtgcgtc cttcctgttc
     9901 gaccgcggcg tcgaggcgcg tcagcccgcc gccgatggcg tctacgatca ggtcgagttc
     9961 gacggagccg ccgccgatgg ccgcgggctt ctcgatccgg acaagcgggc caggctcctg
    10021 gcccgcgaaa taaaaggcgg cggcggcgcc gaggagcagc aggacgaggg cgatgacgag
    10081 cagcggccgt gtaggcataa ggcgtgagaa gcttgacccc ggacgcgcga ggccgtaaaa
    10141 ttagaggttc cagcccgacg ggtgccggta tcgtctaggg gtcaggacac gtggttctca
    10201 gccacgggac cggggttcga atccccgtac cggtaccatc catcatacaa tccggcgacg
    10261 cccctgctcc aggcgcgcgc tccggccgcc cgccggcgcg gtgcgcggac cggggctgat
    10321 agaggaacag agcggccggc atgaggcgct ccctgtgcgc gacactcgcc atcggctgcg
    10381 cgctgggcgc ggcggcggcc gcgctcgcct gggccctcgg ccagaccgcc ttcctccgca
    10441 cggtcgagct gaagacgtac gactaccgcg tcaggctggc cgccgacccc gcgtcggctc
    10501 gccgcgacct cgtcctcgtg agcatcgacg acagcagcat caggcggctc gagccgcagg
    10561 tcggccggtg gccgtggccg cgcctggtgc acgcctcgct tatcgattac ctgcgccgcg
    10621 cgcccgcgaa ggccatcgtg tacgacgtcc tgttcaccga gcgcgaccgg cggacgttcc
    10681 aggccggcgg ggacacgtgg accggcgagg aatcggacct ggcccttgcc acctccgcgt
    10741 cgcgcgccgg gaacgtcgtg ttcgcggcgg acgtgacggc cgacgaggtc gcgctcgaag
    10801 acgtccgggg gctcagcgcg ttgcgcggga gccggttttt cgaggcggat ccggctttcg
    10861 aatccaggcc aggcgtgatc ctgccctacg ccgagctgac cgaggccgcg gccgccgtcg
    10921 gccattcgct cactatctac gacgccgacg gccccgtgcg ccggacggcg ccgttcgtgc
    10981 gcgtcggcac ggtgggcgtg ccgtcgattg ccgtggccgc cgtcatggtg gccgggcccg
    11041 tggcgcgcga gtcgatccgg cgcaatggcg acacgctcga ggtgggccgc gcggccgcgc
    11101 cgctggtcgc cgcgcgcatc ccgagcttct acggccccgc agccgacgcc aggcggctgc
    11161 tcatccggtt cacgggcccg gtcctcaagg acggcaggcc gacctacgcg gactactcgt
    11221 tctacgacct gttctactcc gagcagcagc tcctggcgga gcaggccccg gccgtcgatc
    11281 cggccgtttt ccgcgacaag atcgtcgtcg tcggcacgac ggccgccggg ctgcgggatc
    11341 tgttcacggt cccgttcgcc gaaggcagca tgcccggcat gcaggtgcac gccaacgtca
    11401 tcgacaacct gctgtcgggg cggttcatgc ggcccctcgg ccgcgcggga aacctgggcg
    11461 tgctgctagc gtgcgcgctc gccgtcggca tcgccggcgc gctgacggcg gtggggccga
    11521 cgcttgcggc ggcggcgctg atgatcgcgg ggctcgcctg ggcgtcgtac ctgctcatag
    11581 gccagggcgt gtggatcggc gtggccgagc ccgtgctcgg cgccaccttc gcggcgttcg
    11641 gcgggacggc gtaccactac ttcgccgagg gccgcgagaa gcggcgcgtc aagaagctgt
    11701 tctcgcgctt cgtctcccgg gacgtgtacg agcagctgct ggccgacccg gccggggccc
    11761 ggatcggagg cgcccggcgc gagatgagcg tgctcttcgc ggacatccgg gggttcacgg
    11821 cgctcaccga gcgcggccag cccgaggcgg tggtcgcgca gctcaacgag tacttctcgg
    11881 cgatggtgga cgtcgtgttc cgccaccgcg gcacgatcga caagttcgtc ggcgacatgg
    11941 tgatggcgct cttcggggcg ccagtgccgg acgcggatca cgccgacaac gcggtgaagg
    12001 cggcgctcgg gatgctcgcg gccctcggcg ggctgaacgc gaagtgggcc gccgaagggc
    12061 ggccggccct ggacatcggc atcggcgtca acagcggtga catgctggcg ggcaacatcg
    12121 gatccgatac catcatgagc tacaccgtca tcggggacgc ggtcaacctc ggcgcgcgcc
    12181 tcgagtcgct gaacaaggag tacgggaccc gcctcatcgt cagcgacgcg acgcgcaccc
    12241 ggctcaaggg ccggtacgac ttgcggcccc tcggctcggt gacggtcaaa gggaagtcgg
    12301 aggaagtagc catcttcgcg gtgctgcccc cgtcgtagcc cgggagaaca catgccatgc
    12361 gagccgttca cgcccctcag cgcctgcagt ccgcccgcag ccggcgcctg cgcctcctgc
    12421 gtccgctcgc cgccccgctc gccgcctggg cgctcgtcgt gcccggcgcg gccctggcag
    12481 cgccggccgc gactcaactg atcaagatcc ccaagaagcc gaagctcgac accgtcgtca
    12541 agcgcgtcga gcagctgcgc gagctgcagg tcaccgacga ggaggagcgc cagctcggcg
    12601 ccggcgtgag cgagcgcgtg cgcgagcgct acggcgtggt ccaggatgcc gcggtccacc
    12661 gctacgtcac gctggtcggc acgctcctgg cgcagaagag cgatcgcccc cgcctccact
    12721 tcaccttcgt cgtcctcgac accgacggcg tcaacgcctt cgccgcgccc ggcggctaca
    12781 tccacgtcac ccgcggcgcg ctcggcctga tgtcctccga ggcggagctg gccggcgtgc
    12841 tggcccacga gatcgtgcac gtcaccgaga agcacacgat ccgggccatc cagaagaaca
    12901 aggcgatcca gatgaccgcc gaggagagca tcaagtcgaa caaggcgctc tggaacaagc
    12961 tggtggacgt cacctcggaa atggtgctcg ccgggttcgg gcgcggcgag gagctggagg
    13021 cggacgagaa gggcctcctg gtcgccaatg ccgccggcta cgcgcccgcg ggcctcggcg
    13081 gcttcctcaa gcgcctcgag gcccgcaatc ggacgagttc cggcaggcag ggcctcttcg
    13141 cgtcgcaccc cgagatgaag gagcgcctcg accggatcga gcgggagatc ggatcgcgca
    13201 agctcgcgtc cgcggccgtg ctcggggacc gctacagggc ggcgattccc ttcaccgccg
    13261 cggcggcggc cgacatcgcg gcggtggagg acggcgcggc ggggctcgcc ggcggcggca
    13321 agccgaagaa gaaggggttc ggcctcggca gcctcgtcaa gcctggaggg gccgaaaaaa
    13381 gatcggccga ggtcgtcggg tcgggcgcct cgcgcggggt ggacaccgag cgcgacgcca
    13441 aaggcggccc ggtcaagacg ctggtttcgg tgacgatcac tccggccgaa ctcgaggcgt
    13501 tccgggcgca ggggaacctc ctgtcgtgag cgcgccggcc ctccgcatcg ccatcggcgc
    13561 cgtcatcctc gccgcaacgc tcgtcgtccg cgccgcgagc gtcaaccgcc tcgtccgccg
    13621 caagctgcgc ctgacactgc tgctggccct cgtctacacc gcggccgtcg tcgtgctcgg
    13681 ccgctacggg ctgtcggccg ccatggacgt gcgcgtgctc tcggtggcgc agctctgcct
    13741 ggcgctcgcg ctcatcaatc tggccgtcgt gacggccttc aacccgctgc gcgccgaccg
    13801 cgtgccggag cgattcccgg cgatcgtgca ggacacgatc gtcatcgggc tgttcctgct
    13861 cgtcggcacc gtcgtgatgc gcgagaagtt cctgacgacg acggccgtga gcgccgtcgt
    13921 catcggcttc gcgctgcagg acaccctggg caacatgttc gccggcctgg cgatccaggt
    13981 ggagaagccc ttccgcgtcg ggcactgggt ggccgtcggg ccgcacgtcg ggctcgtggc
    14041 ggaggtgacc tggcgggcga cgaagctcaa gacgaaggcc gggaacctcg tcatcctgcc
    14101 gaacgccttc atggccaagg aggccatcac caactactcg gagccgtccg cgctcacgcg
    14161 gctcgatgtg gaggtcggcg tgagctacgg ttcgccgccg aaccaggtga agcaggcgct
    14221 cctcgaggcg atcccgaacg cgccccttgc gctgaaggac cccccgcccg acgtcatcgt
    14281 cgccgacttc ggggcgtccg ccgtgatcta ccgcgtccgg ttctggacga acgacttctc
    14341 ccgcgacagc ctggcccgcg accaggtccg gacggccgtc tactacgcgt tgaggcggca
    14401 cgggctcgag atcccgttcc cgatccagac cgagatccac agggagccgg agtccgagcc
    14461 ggaatccgcg cggaacggcc gggtcaacga ggcgctgcgg caggtcgatc tcttccagcc
    14521 cctgtcgatc gacgagcggt acgccctggc cgcgcgggcc ggcgaacgcc tcttcggcca
    14581 gggcgagacg atcgtccgcc agggcgagac cggcgggtcg atgtacgtcg tccgccgggg
    14641 ccgcgtccgc gtgatcgagg catcgggccg ggaactcgcc gttttctcgg ccggcgggta
    14701 cttcggcgag atgtcgatgc tgaccgggca gccgaggacg gcgtcggtcc aggccgtcga
    14761 ggactcggac gtcgtcgagc tgacggcgga gtcgctgcgg gaagtcgccc tcgtcaaccc
    14821 ggacgtcctc aggcgcatca gcgatgtcgt ggccgcgcgc caggccgacc tcgaacggca
    14881 gaaggccgag ggcgctccgg gacgcccggt tgccgccgag gcctcgcggt cgctgctctc
    14941 gcgcatccag gcgttcctgc gcctgccggc cctgattggc gactagcggc cccgtcggcc
    15001 gcgaacggat ccgatccgac gagcgtggag cgatccatga gcatctcccg gcaaccggct
    15061 gcgatcgcat tgatgatcct gatgagcgcg gcggccgcgc cctgggccga gcccgacccg
    15121 caggcccgcg ggcggcttcc cccgccggcg ttcgccgacc ccggccgcgt caccaggctc
    15181 gagcgggcat tccccgagat cgaccggctg ttcaagacct tcgccgagcg cgcgcgcgtg
    15241 ccggggatcg cgtacgggat cgtgatcgac ggacggctcg cccacgccgg cgcggcaggc
    15301 gtccgcgacg tccggacgaa ggatccggtc gatctggaca cggtgttccg catcgcgtcg
    15361 atgacgaaga gcttcacggc gctgtgcgtc atgcggctgc gcgacgaagg gaagctcggg
    15421 ctcgacgacg cggcggatcg atacgtcccg gaactcgccg gcctcgcgcc gccgaccgcc
    15481 gattctcccc gcgtcacgat ccggcacctc ctctcgcacg ccgcgggctt tcctgaagac
    15541 aacccctggg gcgatcgcca gctcgccgcg accgacgacg ggatggcgcg gatgctggag
    15601 cgaggcatcc cgttctcgac ggccacggga acggcctacg agtactcgaa ctacgggttc
    15661 gcgatcctcg gccgggtggt ctcgcgcgcg gccggcatgc cgtacgaggc ctacgtcgcg
    15721 gatgccatcc tgaagccgct cggcatgagc gcgacgacgc tggaggccgc cagcgtgccc
    15781 ccgccgcgcc tggcgcgcgg ctaccggctg gaggagggcc gctgggtgga cgaaccgctg
    15841 ctgcctcacg gcgcgttcgg cgccatgggc ggcatgctga cgtcggcgcg cgacctggcg
    15901 gcctacgtgg cgttcctgtc gtcggcctgg cctccgcgcg atgatcccga caccggccct
    15961 gtcaggcgcg cgtcgctgcg cgagatgcag caggtgtggc gcccgtcgcc atcggtcgcg
    16021 cgccgcagct ccgtcgacgg gccgctgcag ctcagcgcgg gcgggtacgg attcgggctg
    16081 cgcgtctggc agagctgcgg attcgggcat ctggtggcgc acagcggggg cctccccggg
    16141 ttcggatcgc acatgcgctg gctgcccgag tacggcgtgg cgctcttcgc catgggcaac
    16201 ctcacgtaca cgaactgggg gctcgccgcc gacgatgcca tcgaggccct cgccaggacg
    16261 ggagggctcc agccgagggt ggcgcagccg tcggtcgcgc tcctcgatgc cagggcccgc
    16321 gtgatccggc tgatcgatcg gtgggatgag cgcgaagccg cggctgtcgc ggccgacaac
    16381 ctgtttctcg acgagccggc cgacaggcgc cgggcgcagc tcgagtcgat ccgggccgcg
    16441 cagggcgcgt gccgggcgaa cctgggcttc aacgtcgaaa acgccttgcg cgggacgtgg
    16501 accgagacat gcgaccgtgg ctggctgcgg gtgggaatca cgctcgcgcc gaccgagccg
    16561 ccgctcgtgc aggagtgggt cgtcgtgccc gttcggccgc tggcgccggg cctcgacgcg
    16621 gcggcggcgg ccatcgcgcg cgccgtctcg gcgcaacgcc gcggcccgct ctcgatcaag
    16681 ctcggcgccg gagtcgcgcc ggaggaggcg cgccgcctcc tggacagcgc agcggcgtgg
    16741 ggagcctgcc gcgtgggcga ggtgctcgcc agcgacggcg agcgcgccgc cacgatccgc
    16801 ctcgcgtgcg cgaagggcga cctcgacctg tcactcgccc tcgacccggc gacgcgcgtg
    16861 ctgacgcgcc tcgccctcgc tcccgcggca ggcggaacct gcgttcccta ggcgcgaacg
    16921 ccggtgacta ctgcgcccag acgaggatgc gctggccgcc ggcgtccttg tggtccagga
    16981 taaggccagg cccccgcttc tcgaggtccg cggccgtgag cttcagcctg acgggagcct
    17041 cgtcgccgtc gtgctggatc ttgatcgggt gtcccttcga catccggagc agccagagcg
    17101 ggacgttgaa gcggacgacc cggccctggt cgggatccca cacgaggatg tgaatggcgt
    17161 cgatgggttc gcccggcttc gcgggccgat cgttgctcac gatcggctcc ccgtccttga
    17221 tggccaggta gggcttctgg ccttggaacc ggctcgcggc cagcgcgaac tcctgcgcgg
    17281 cgtcctcctc ggtggcagcg gtcgcctgga tggcgaactg ctggtagacg aagtagccgg
    17341 cgacggcgat cagacccgcc cccacgatca gcgcgaaggc gccgatcccg aggacgatga
    17401 cgggccaggg agtcttcttc gccatctgct ggtactgacg ctcgcggcgg gcgtatgttc
    17461 cccgcccggt cacttgcgcg acgtcagctc cgggctcgcg agccgccgca gccgggagtc
    17521 gcgcccgcgg cgggcctcgt gcgcacttcg tatcggcgcc agcccccggt ggcctcagcc
    17581 gcggttcccc aggcccggaa tcagcgcgcc gctcgcgtat aatcgcggtc cgcgcgactc
    17641 acgcgaggtg tcatgtcccg aagacccgcg ccgcgccgct accggaccat cgacgcccac
    17701 gccgccggcg agcccctccg cctgattgtc gaaggctact ccgcgccggc cggcggcaca
    17761 atgctcgaga agcgcgcctg ggcggcgcgc cacgccgacc acgtccggcg ggcgctgatg
    17821 ctcgagcccc gggggcacgc ggacatgtac ggcgccgtcc tcaccgagcc cgtcaccggc
    17881 gcgtcggacg ccggcgtgct cttcatgcac aacgagggct acagcacgat gtgcggccac
    17941 ggcatcatcg ccgcgacgac catcgcgctc gagcgggggc tgatccatcc gcggcgggcc
    18001 ggggagatcg tgttcgacac gccggccggg cccgtgcggg ccagggcggc ggcggaggcg
    18061 cgcggggacg gcgccgtccg ggttgcgcac gtcgcgttcg acaacgtgcc gtcgttcgtg
    18121 ctcgcgcccg gcctggagct gaaggcccgg ggacgaagcc tccgcgcgga catcgcgttc
    18181 ggcggcgcgt tctacgcgat cgtggatgcc gagtcggccg gagtgcccat cacgggttcg
    18241 cggctggcgg acctgcggag cctcgggatg gagatcgcgc gggcggccga ggcggccgtg
    18301 cccgtggtgc atccgctcga gaagggcctg aagggcatct acggcacgat cttcaccggc
    18361 ccgccggggc gcgaggacgc cgacctgcgc aacgtgacga tcttcgccga tgccgaggtc
    18421 gatcgctcgc cgtgcggcac cggaacgtgc gccgtgatgg cggtgctcgc cgacatgggg
    18481 ctcctgccgg aggatcgcgc cttcgggcac gagagcgtga tcgggacgct gttccggggg
    18541 cgagtcgcgg gccggacgcg cgtcggcggg ttcgacgcga tcctgcccga gatcgagggg
    18601 tcggcctgga tcaccggcga gcacgtcttc ctgatcgatg acgacgaccc gctgcgcgcc
    18661 gggttcaggc tgtagcggcg ccggcggcta cttcaaggcc gccagcttcg ccatgtcgag
    18721 ccggccggca acggcctgcg cgtccttgat gtccgcgccc gagccttcga cggtgacgag
    18781 gaaccgctct ccgacgatca cgccgagttc ggccgtcttc gactcactgt tccacttctc
    18841 ccagccgggc tgtcccttca ccttgacggc cttctcgtag ccgctcgtcg actccttcga
    18901 gtagcccgcc gccaggaaca tctggtaggg catcgtcagg agcctggaca tcgcggtgtc
    18961 gacgatcttc acttcgatgc gcgcgtcgcc cttggtgtag cccgcgctgg cctgggagaa
    19021 gcggaccggc gccgtcgtgc tctcgccctc gggctcctcc ttctcccagc ccgggacgtc
    19081 cggcagcagc tcgatgagcg atctgaagct caccggctcg tacgccttgc cgtcgggcga
    19141 cttctgcatg gattccatcg cccgggcgaa ctcctgcatg cccttgacga ggtcagcctc
    19201 accgccgccc cgttcctccg tggcggactg ctgatcctgc gtggcaggtt cccgcgcggc
    19261 gccggccgcc tgctgctgtg cctctttctt gccgccgcac cctgcggccg ccagcgcgac
    19321 gactccggcc agacagacga tcatgaatct ccgcatccaa cgcctccctg tgttgacgat
    19381 cggccgcccg gattgtacct cggctgccgg gtgtccggcc cgcctctccg tcagcgcggt
    19441 gccgcgagtg ttccgttgaa ccgaacccgc cgcctgatgc gcgcctcccc gtcagcgcgg
    19501 cgctgcgagc ccgctgtcgt cagcggccga ggtgcaggcg cacgagccgc tcgaccagct
    19561 gcgccagcgt ctcgccgccg agggcgaagg cgcccgtttc ctgcgaccag gccagcttgt
    19621 agctctgcac gagcgccgac acccagatct ggcggaccgg ggcgttgggg ctgatgacga
    19681 aggtcgcatt ggaaggctct tcgaattcga tcttgagcac gccgccctcc agatcgacct
    19741 cgaacccctc ctcgtcggcg aggccgagca ggccgcgctg cagcgcctcg atggccgatt
    19801 cggctttcgc cgcgaactca tggtcggtca cgccgtccgg catgggtcga tgatagcaga
    19861 cgggcgcggc gccgcggccc ggcgccgagc cgaaccagag cgcctcgttc cgcgtctact
    19921 gatccaggtg ggctcgaacg cccccggggt tccaggctga gcggcgctcg gccaaggtca
    19981 ggcgcggatg acgccgatct cgggcgtgag ctgtctgccg tcggcgtccg ccgggggtca
    20041 gtagagccat tcgattcgca cgagaggagg acgtcatgtt cgagtggaac gtgtcgagca
    20101 acgcggactg gctcgtggcc gcgctgctca tcgtggccgt cattgccgcc ctggcctgag
    20161 cgggccttcc gcctcgccct cgcgcctgca gcgggcgggc gcgctcactt cggcgggcct
    20221 gtgccggcct ccgacgcggc gacgtacgtt ttgcgcgccc gggccttcat gcccgggcgc
    20281 ttcacgttca cggtcagctg atgcaccctg ccgtcgagca cggccggcgt gaagcccagc
    20341 acgtactggc tgtgcagctc ctgggcgatg cgcgtgaacg tgctgctcag gttcgaggtc
    20401 tccctgagca ggaagtaacc tccgccggtc tcctccgcga gcctccggac cacgcggtcg
    20461 gggctcgtgc ggacgcggcg gattccgttg aagtaatcgg actcgaggcc aatgccgtag
    20521 atcatgacct cgccggccac ggcccgggcg agcacgtcgc cccggccgcg ccggctggcc
    20581 gtgtcctccc cgtctgtcag caccacgatc acgcgccggc cctcaatcgg ggcgagccga
    20641 tcgagggatt cgtcgagggc gtcgtagagg cgggtggggt tgccgaaatc cagctcgccg
    20701 agggcggcga tcagggcgtc ccggtccgag gtgaactcgc cgctgaactg gatcttgtcg
    20761 ttgaacgcgc cgacctgggc cttgtcctgc ggcagcaggc ggatcaggaa ctgctcggcg
    20821 gcggccttca cgagcttgag cgacgaggtc atgctgccgc tgctgtcgag catgacgacc
    20881 gcggtgaagg gctgcgtctg gttctcgaag acgaggaggt cctggcggac gccgttgtcg
    20941 aggacctcga actccgcgcg cgtcagatcc ggcaccagcg cgccgcgcgc gtcggtgacc
    21001 gtcgcgtaga tcgggaccat cctcgcgccg ctgcggaaca ctccgccctg ccgagcctcg
    21061 gggcgcggcg cggcggcgcc agcggcctcg ggcccgcgcc ctgcggcagc ggccgccagc
    21121 gccgcggcgg ctgccgccgc cagaaatcgc cgggtgcggc tcatggaccc agcctagcaa
    21181 gccgcgtcga ggcagacaac ctggggaacg gcgcgcccag gggctacaat gatgcgggcg
    21241 cgaagtccgc gggccgccgg cgcccgcgga ccggcggggc gccgccgcct ccggacacga
    21301 agaggctgcc tgacggagcg cggcgctcca ggcggcctgc cgggtgaatc gcccgggagt
    21361 cgagccgcgc cggcgcggcc tggaggcccg cagcctcgag agggcgtgcg ccgtttggtt
    21421 ccagaggcac agggcaggcc ggaggagcgg cctggagcat ggctgacgcg agaccgccgg
    21481 tcatcgtgaa cctcgagtgg gacggcgacc aggccttcac ggcgcacgac ggcgtccacg
    21541 aatgggtgct ggacggccgg aaccaggccg gcccgacgcc ggtcgtggcc ctggcgtcga
    21601 gcctcgccgg gtgcatcgcc atcgacatcg tccacatcct tgcgcgcgga cgcttcgatg
    21661 ttgtggcgct cgaggtgaac ctgaggggcc accgcgccga caccgagccg aggcggttcg
    21721 tgcgggtcga gatgcgcatc atcctggaca cgaacgcccc gcaggagcac atcgaccgcg
    21781 cgatcgcgct ctcgcgcgag aagtactgct cggtgtggca ttcgatgcgc caggacatcg
    21841 agctcgagac gaccgtggtc cgggcccgcc cccgcagcga ggcgagttga gccgcggccg
    21901 ggcgccgcgt ccgccgccgg gacgcccggc cccttcgacg cgctggcggg cggagtcccg
    21961 cccctcgggc cgcgcatcgt gagacgctgg cggcgcaccc ggccgaagcc ggaggacgac
    22021 catgaagcct caggatcttg tcgccccgtt cctcgtcacc gacggcggcc ggttctcgct
    22081 gaaggaccat ccgaccggca gcgggtcgtg gaagcaatcg aagaaggaca cccgcaagct
    22141 cctcgagcag ggagtggagc ggctcgcggc gctccaggag aggctatacg cccaggacac
    22201 ctggggcgtc ctgctgatct tccagggcat ggacgcggcc gggaaggaca gcgtcatcgc
    22261 ccacgtcatg tcgggcgtca acccgcaggg atgccaggtc tgctcgttca agacgccgtc
    22321 ggccgaagaa ctcgaccacg acttcatgtg gcggacgacg aaggccctgc ccgagcgcgg
    22381 ccgcatcggc atcttcaacc ggtcctacta cgaggagacc ctcgtggtgc gcgtgcaccc
    22441 cgagatcctc gaccgcgcga agctccccgc agccgccagg acgaagacca tctggcgcga
    22501 ccgcttcgag gatatcaacg cgttcgagcg ctacctggcg cgcaacggct acctcatccg
    22561 caagttcttc ctccacgtgt cgaaagagga gcagaaggag cggttcctcg agcggattgc
    22621 ccggccggag aagaactgga agttctcggc ggccgacgtg aaggagcggg agcgctggga
    22681 cgactacatg gcggcctacg aggacaccat ccggcacacc gccaccctgc acgcgccctg
    22741 gatcgtcgtg ccctccgaca gcaagcggtt cgcccgcatc gtggtggcgt cggccatcgt
    22801 cgaggccctg gagggcctcg acctggcgtt tccgacgctt tccccggccc ggaagaggga
    22861 gctccgggca tgccgcgccg cgctggagcg cgagggagcg ccgcctcgag cctagcaagt
    22921 tacagcctgt caaggggtta gaggaaccgt tcgcgcggag gcgggcggcg ctccggggag
    22981 cggcaaactc accggggcct ggaaccgtgc agatcgcggc tgaaatcccc gaaaaagcgg
    23041 gtaagaatac cgcaggattc agccatgatc aacgtgtccg ccatccagat gcagcaattc
    23101 ggggttcggt tctaccaggc ctcgctgacc gccagggaca tcgacaggct cgtgcgcttc
    23161 gaggtgctga gctacgggca gccgcatcag ccccccgtgc gcgggaagaa gcggggcgct
    23221 gtcgcctcca aggtgaactg ggacctgctc gagcgtcgga tcgccgccag cgaacaggcg
    23281 taccagcggc agatcatccg ccggaagatc gacgagctcg tcggctacta cgagcagtgc
    23341 cgccaggcgc gcgacctgcc ctcgatcccg ggcgcggtca tcatctcgtc cgacgagaag
    23401 ctcggcttct ctcccgtcga ggaaggctcg agcctgggcg tcctgaaggt gcccgagcgc
    23461 gagggcatcc tgcgggccat cgacggccag caccgccttc tcgcgatgca cgcggacatc
    23521 gaccggttcg gcgaggaggc gttctcggtg ccggccgtga tcttcgacca gctgcccgag
    23581 gaccacatcg tccagatgtt cgtgacgatc aacgcgaagc acacgcggct gaactcgtcg
    23641 cacctggtaa gcctgtccgg ccgccagctg taccgggacg ccaacctcgc gctcgcgcac
    23701 gacatcgtcc gggccctgaa cgatcgcgac gactcgccgc tccacatgga catcaagctg
    23761 ctcggcgtgg gccatggcag cgtggcgcag gcgccgctcg cgcaggagct caagcgtctc
    23821 ttccagggcg acgccttcgg cgggccgcgc cggacccgcg agttccatga cgaggccacg
    23881 cgcttcttcg tcaactactt caagcaggtc gcgctggtct tcggcgcggc gtggaacggg
    23941 cggaagtaca gcatcaagtc gtcgtcggcc cttcgcgcct tcctgcgcgt gtcgccggag
    24001 gtcgtgcggc ggctggacga ggagcacgcc gaccgcgccg acttcagggc catcggccgc
    24061 gtcatcgcgc cgtggggcca ccgcatcggc gaccttcgct tcgagaccca gggcgcgtgg
    24121 agcaaggggt cctccgtgga ggcgctggcc aaggagctgc gcctcgcgct gtactacccc
    24181 gaaggggcca tggggtagga cccatcccga tgaagacgcg caggcaggcc gccatcctcg
    24241 atctcgtcga tcgcgtgccg ctgtcgagcc aggaaggact caggcggcgc ctcaaggcgc
    24301 tggggttctc ggtgacccag gccacgctct cccgcgacat ccgcgatctc g
//
LOCUS       DAOSIF010000005        22858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_2492_length_22858_cov_4.345042, whole genome shotgun sequence.
ACCESSION   DAOSIF010000005 DAOSIF010000000
VERSION     DAOSIF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 22858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22858
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_22858_cov_4.345042"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLT35_00440"
     CDS             complement(<1..405)
                     /locus_tag="PLT35_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG27718.1"
                     /translation="MARKLKWDVWLFATTLVLVAVSILMVYSASEAIAARQGEENPFV
                     AKQVLFAVLGLVSMFAAMHVDYTRYRNPRVILAVIALTVVALLAVLIVGPKINGTRRW
                     FGVGGIGIQPSELAKLTLILAAAAFLEQRMRRI"
     gene            complement(587..2002)
                     /gene="murD"
                     /locus_tag="PLT35_00445"
     CDS             complement(587..2002)
                     /gene="murD"
                     /locus_tag="PLT35_00445"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG27719.1"
                     /translation="MTGPQSTGGFSVRGMRAVVVGGGASGTAAARLLAERGASVTLTD
                     VRAAIDSQVALQAAGVALVLGSNPAALAAGADLVVLSPGVPITQPAVAAARRAGVPVI
                     GEIELASRWLRGRIVAITGTKGKSTTTALTGRILAEAGYDAPVGGNIGTPLATQVASS
                     TPATIHVVEVSSFQLETTDTFHPWIAALLNLTEDHLDRHASFEEYAAAKARVFARQTE
                     ADVAVVNADDARAVAVAETGRARVVRFARDARIDAGVTVSGGWIVERGGSGDERLMRA
                     GDVRLVGPHLLADVLAAAAVARAAGAPAGAVAGAVSSFRGLEHAMEVVADIGGVRYIN
                     DSKATNVDAARHAIETFGPGLVVIMGGRFKGGDLARLRPALAARRATVVAIGEAAPLV
                     QDALDGAATVRLAGSMADAVRTAADAAAPGGSVLLAPACASFDMFEDYAARGRAFKAE
                     VEALRREGERRKEERRPRREP"
     gene            complement(1999..3408)
                     /gene="murF"
                     /locus_tag="PLT35_00450"
     CDS             complement(1999..3408)
                     /gene="murF"
                     /locus_tag="PLT35_00450"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOG27720.1"
                     /translation="MSAAVTLTAAQAAKAMGGVLAAGPAGAPLDAFSIDTRTLRAGDL
                     YFAIIGKRFDGHRFVADALGAGAAGVVVSAAGAVPRRLERDAIVIRVEDTTRALQRLA
                     QHVRRESRAQVVAVTGSVGKTTTKEMTADLLALGHRVFRAPGNFNNHIGLPLSLLELR
                     RGPDIAVVELGMSHAGEIRALVEVAEPDVRVWTTVAEVHSAFFASIEAIADAKAEILE
                     GADRASLVVANAADPLVMARVRATAAQVVTFGVGVPADVSAAHVADLGVAGTRATVTT
                     RAGAFELDVPLFGRGHLANALAAIAVASHYGVPLADAASRVAAMKASPRRGEVWRLAG
                     GVTLVDDSYNSSPRALEAALDALAAERGCERRLAVLGEMLELGVDTVAFHERAGHAAA
                     RAGLAQLLTVGGAPARAMADAAVTGGIPAEAVAHFATSDEAADAAARAVRPGDVVLVK
                     GSRGVRTDVVADRLKAGAP"
     gene            complement(3405..4901)
                     /locus_tag="PLT35_00455"
     CDS             complement(3405..4901)
                     /locus_tag="PLT35_00455"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG27721.1"
                     /translation="MTLRDLLTVLDGPGLRAPGAGGAETPAGGVPVLDVVCDSRAVSP
                     GAVFVALRGQRVDGAAFAAQAASTGACAVVSEADAPPQFPVPWVRVTDARLALALLAA
                     SVHGHPSRSMRVVGITGTNGKTTTSFLAQAVFEAAGIRCGLLGTVHYRVGAEAQDAVR
                     TTPEAPELQRLLREMAGAGCGACVMEVSSHALALKRADGTEFAAAAFTNLTRDHLDFH
                     GDMDRYFAAKRRLFDLLPDGAPSIVNVDDRRGASLAASRRPCVTFAVDAPADVRPAAM
                     PASLAALDFSVAARGVTLRISSPLAGRFNLSNLLAAAAIGVALDLPAQAIEAGLSSVA
                     SVPGRFQVVSSAADDVTVIVDYAHTDDALRNVLEAVRAMAGARLTTVFGCGGDRDRAK
                     RPLMGDVAARLSDLVVVTSDNPRSEDPERILDDIERGIPPHRPRLRLADRRAAIGRAI
                     EGADAGDVVVVAGKGHEKNQVIGDRALPFDDAAVARDALDRRRLRSTP"
     gene            complement(4898..6919)
                     /locus_tag="PLT35_00460"
     CDS             complement(4898..6919)
                     /locus_tag="PLT35_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOG27722.1"
                     /translation="MNEPVGRAWLAAMRARVLVMAACLALWAGAIEARLVHLQVFQRD
                     ALEAAAAGQRNRTLAADARRGDIVDRAGRLLATSVDATSICAIPKEIDDPGAVVAAVC
                     ATLGDCTAEERRLFAQRLGQKDRAFAFLRRQASIEAAAQVERLGLQGVFMKAEPRRYY
                     PNRELAAHVLGHLGTDNQGLAGIEQSYESRIRGTAGQIIVKVDANKSAFSRVEIPPVP
                     GDTIELTIDAYLQHIAERELERGVAEHRALGGTVIVMAPRTGEILAMANAPAVNPNAY
                     RDYTREAWMNRAVQAIYEPGSTFKIVTASAAMEERTFRPTDAFDVSAGQIRLAGRVVS
                     DTHRYGVLSFTDVIVKSSNVGAIKIGLGVGAERLGRYARRFGFGERLSVDLPGETRGL
                     LLDSAQWNQSGLMSISMGYQIGVTPLQMAAAVSAVANGGVLLRPRIVRALRRGGVRTG
                     VMPQELHRPISEATASEIVSIMEQVVERGTGTAARVAGYSVAGKTGTASKAVARGYSK
                     TDYQSSFVGFVPSRRPAFTILVVIDSPHAGAIYGGAVAAPVFQRIADAALRYAGVPPT
                     INPLPPVLVKASLEDPPPVELVQASATAGPPRISVLTAPPTVPDVRGLGAREAARRLS
                     RLGLLVRLTGDGVVIAQDPAAGSVLEPGRVCRVWLGRLSSLPPAPAPAP"
     gene            complement(6912..7349)
                     /locus_tag="PLT35_00465"
     CDS             complement(6912..7349)
                     /locus_tag="PLT35_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOG27723.1"
                     /translation="MANRHEPADAIAYSIPREVENHPVRVVDRARRRDMLRLAGVVAL
                     LLVGLLVVAGLHSEWLALGYDLARLEKARAAGEAEQRHLRLELETLRAPERIERLAIQ
                     DLNLVAPSQTEAVVLERVRQAAAPARTLVARRQAPEGGAGGHE"
     gene            complement(7354..8232)
                     /gene="rsmH"
                     /locus_tag="PLT35_00470"
     CDS             complement(7354..8232)
                     /gene="rsmH"
                     /locus_tag="PLT35_00470"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG27724.1"
                     /translation="MPVHVPVMAAEVVEALRPARGGLFVDCTVGAGGHARALLAAGAT
                     RLVGIDRDGEALDRAREALAEWGDRVALVHGDYRDLARLLDERGVTTVDGAVADLGVS
                     SLQLDGAGRGFSFRRDEALDMRMDTSRGPTAADLVASAGEQVLSDVIFRFGEERRSRA
                     IAHAIVEARRTAPVDTTARLAAIVRRVVGRSGRIDPATRTFQAIRIWVNGELDGLDAF
                     VRAACGRLGAGARLGVITFHSLEDRPVKHTLRALAGESGPLRLVTKKAVVPGAEERRA
                     NPRARSARLRVIERME"
     gene            complement(8254..8709)
                     /locus_tag="PLT35_00475"
     CDS             complement(8254..8709)
                     /locus_tag="PLT35_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27725.1"
                     /translation="MEQTAVLRGNAPARIDDKGRLKIPTAFKALVEARHGRDLFVTSL
                     SGESVRIYPMPVWIEIERKLAQIPSTHPSRLRFFSRVNYYGQTAELDNQGRVLIHPRL
                     REAAGMAGEVDVFGQYDHLEVWNHERFVTKLANDPYTDADARALADFGI"
     gene            8933..10024
                     /locus_tag="PLT35_00480"
     CDS             8933..10024
                     /locus_tag="PLT35_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOG27726.1"
                     /translation="MLRTALIGFPSTGKTALFQLMTSAHGAPRAAHGRTDVTLGVARV
                     PDPRLDVLTAMYRPRKRVPAMVEFADIPGRPSGSGAGALLDVAAYRNADALVHVVRAF
                     EDPAVPHAAGSIDPLRDARAMEDELILADLAVAEKRLDRLAKDLKKARTAELEGEQAV
                     LAACRAHLERGTALRDLELPDDDRRRLRGFQFLSAKPLLLVLNADERDLARGLDRLPE
                     RYGLAGPGTGPRTRAVAVCAKIELEVAQLDEADAAAFLADLGLKASGLDRVIRASYDL
                     LGYVSFFTVGEDECRAWSVPRGTPAQAAAGEIHTDLSRGFIRAEVVAFDRLAGRGSMA
                     ACRDHGEVRLEGKDYPVADGDVITVRFAT"
     gene            10083..11126
                     /locus_tag="PLT35_00485"
     CDS             10083..11126
                     /locus_tag="PLT35_00485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG27727.1"
                     /translation="MPVRTILVCEAQVPFVRGGAESHVRQLVDRLRARGYAVDLVSVP
                     FKWYPKEEILAHAAAWRLLDLSESNGTPVDLVIATKFPSYFARHPNKVAWLIHQYRAA
                     YELCGTVYSDFAHADLDAGLRQSLLDLDTRMLGECRRLFANAGNTARRLEKFNGLTAE
                     PLYHPPRVAGRLAPGPSGDYVLSVGRLETVKRHDLAIRALAHAPPRLRLVVAGEGTQD
                     ANLRRLAESCGVAGRVAFEGAVDDARLLALYAGALAVVYAPFDEDFGYVTLEAFLSHK
                     PVVTARDSGGPLEFVEDGTNGCVCDPDPAAVGGAFARLDADRERARALGDAGFERARL
                     ITWDGVIERLVGE"
     gene            11138..12121
                     /locus_tag="PLT35_00490"
     CDS             11138..12121
                     /locus_tag="PLT35_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG27728.1"
                     /translation="MKLIVQIPCLNEAQTLPATLRDIPRAIPGIDVVEVLVIDDGSRD
                     GTAEVARRHGVEHVVRFPQRKGLASAFAAGIDACLALGADVIVNTDGDNQYDGADIPK
                     LIAPLVAGEADIVIGDREVRDLAHMSATRKRLQWLGSWVVRQVSDTKVPDTTSGFRAY
                     TREAAMRMTIISEFTYTLESIIQAGKRRMAIVHVPIRSNPGTRPSRLFSNVWTYIKAS
                     AATIIRVYAMYEPLKVFGLIGLVVFGGGIAVSLRFVYFYLVGEGLGHVQSLILAAVLL
                     IVGFQIGLIGLMADVISGNRKMLEDLIYRVRRMELRQNGAAGGPPPGPDRS"
     gene            12126..12986
                     /locus_tag="PLT35_00495"
     CDS             12126..12986
                     /locus_tag="PLT35_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG27729.1"
                     /translation="MAEPSSVSIVIPAFNEEPAIGPLVAALGAGAAWREIIVVDDGSS
                     DATAARAREAGATVVRHPYNKGNGAAVKTGIRAATGEYVLVVDGDGQHRAADAARLVA
                     RLGEYDLVIGARSSDTQATHARLLGNAALNWLAGSLTGRAIPDLTSGFRGARRTHLRE
                     FLHLLPNGFSTPTTTTLAFIKAGYNVCFEPVEAAARVGSSKIRLARDGVRFLLIVLKI
                     VTLFSPFRVFLPISLAAFAAGAGYAAWTVATQSHVTNSSVLLIMLAVIVFLVGLVSEQ
                     IAALRFEGRE"
     gene            12992..14236
                     /locus_tag="PLT35_00500"
     CDS             12992..14236
                     /locus_tag="PLT35_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27730.1"
                     /translation="MSAQMPRAVWMAVAAGVALRLAFALGYWVDKPLTHDEREYLTLA
                     GNVAAGRGFVHLSPDGSSVPGEHFGRAPLYPLFLAGVVRASGAEGASAIPPAPDAAPA
                     AAVLRAVRAAQSVLGGAIVWLVAWLAGRAAGPRAAASAAALAAVYPPLVWTPAYVWSE
                     TLFSVLALGCAAVLTRTPGSPGGAAASGALGGLSVLTRPAMLFFLPLAFIWLGWRHGW
                     RAAAILTVACLLVVAPWTIRNAREYGRFVLIASEGGITFWTGNHPLAVGEGDLAANPA
                     LKRANADLRARYPGRSPEELEPIYYREAWRFVGEAPGAWLRLVARKAFYTVVPVGPSY
                     RLHSARYYAASALSYAAVLPFALLGLRALWRKGRLPAPLLLLASSSLAVCLVFFPQER
                     FRIPVVDPTLLVAAASWRALRG"
     gene            14241..14978
                     /locus_tag="PLT35_00505"
     CDS             14241..14978
                     /locus_tag="PLT35_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOG27731.1"
                     /translation="MNVLVIVPTYNERENLPLLAREVLDRQPYEMLVVDDDSPDGTGA
                     VADGLARDYPGRLHVMHRAGARGLGLSYLDGFRWALASGADLICQMDADLSHDPRYLP
                     DLVAAASAFDLVLGSRYLHGISVVNWPLRRIVLSSFANAYVRTITGLTTRDSTSGYRC
                     WRRGALARLDLERFLSHRYAFMVETVFEVARAGMRVGEVPIIFVERRRGASKMSARVL
                     AESMIMPWRLVLRNRGRVRPAPGGAAP"
     gene            14975..16231
                     /locus_tag="PLT35_00510"
     CDS             14975..16231
                     /locus_tag="PLT35_00510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG27732.1"
                     /translation="MSRRHVVVMLTTSYPRFPGDAIATFMEPIALGLAARGHQVHIVL
                     PWHPRLARGPAEGGVRFHPFRYAPHPSLNVFGYAAALRADVELRAAAYAAAPLALAAS
                     FRAARRVAREAGATILHGHWLVPSGAVAAAAAGRLPVVISLHGSDVFVAERHAVARRA
                     ARAALGRADALTACSDDLRDRAIAIGADPARSETIPYGVDVERFRPDAARRAETRRAM
                     GADADDPVLFTAGRLVRKKGFGDLIDAVGLLATRWPRLRLVIGGGGDLDAELRERARR
                     LGVADRVEFAGVLAQDEVAARLAAADVAVVPSVHDEAGNVDGLPNTVMEALASGTPLV
                     ATRVGGIPSVVAHGRTGWLVPERDASALANALDRLLADPASRAGLGAAARAEALASHT
                     WARVAERFEAVYDRAACRAEERRGTV"
     gene            16241..16996
                     /locus_tag="PLT35_00515"
     CDS             16241..16996
                     /locus_tag="PLT35_00515"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG27733.1"
                     /translation="MADQQATPGLTVFFPAYNDAGTIASMVVTAVLAASRLTPDVEIL
                     VINDGSRDRTPAILDELARVYPQVRVIHHPTNRGYGGALRSGFAHASKDWIFYTDGDA
                     QYDPWEMGVLWAAMGPGIDMVNGYKISRSDPLHRIVIGRLYHHMVKRLFGLKVRDVDC
                     DFRLMRRTIFDRVTLTKDSGVICLEMMKKIQDAGFTIAESPVHHFHRAYGKSQFFNLR
                     RIARTAADVLKLWWALVVRRSAQPPPAPARSGG"
     gene            16999..18021
                     /locus_tag="PLT35_00520"
     CDS             16999..18021
                     /locus_tag="PLT35_00520"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG27734.1"
                     /translation="MEYEDFYRGRRVMITGGAGFIGSNLARRLVDLGANVLLVDSLIP
                     EYGGNLFNIRGIADRVRLNVADVRQDSTMSYLVRHVEIIFNLAGQVSHIDSMRDPFTD
                     LEINCRSQLSILEACRRNNPAAKVVFAGTRQVYGKPDCLPVTERHLVRPTDVNGINKV
                     AGEYYHLVYDHVFGVRACSLRLTNIYGPRQLVKHDRQGFIGWFIRLAVEDREIQIYGD
                     GSQLRDFVYVDDAADAFLRAGAADACNGEVFNVGGAEPVSHRDLVRLLIEVAGTGRVR
                     FVPWPEEKRAIDIGSFYADSSKFKAATGWEPRVALRDGLARTVAYYRAHLPHYLNPEA
                     QELLPP"
     gene            18018..19109
                     /locus_tag="PLT35_00525"
     CDS             18018..19109
                     /locus_tag="PLT35_00525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG27735.1"
                     /translation="MTLRIPSVRLAPGEDRPAVEAAVRRVVASGWYVLGPEVESFERA
                     FAGACGAAACIGVGTGTDAIAISLRALGIGPGDEVITTPLSAAYTALACMMVGARPVF
                     ADIDPRRLTIDPAAIESAITPRTAAILPVHLYGQPADMRAIAAIAARHGLAIVEDCCQ
                     AHLATCDGQRIGSFGAAAAYSFYPTKNLGALGDGGAITTGDEALAAKMRRIRNGGQTD
                     RYRHAEMGVNSRLDELQAAILAARLPFLAAWTAARRARAAEYRRLLAGSAVDVPPELD
                     AGHVYHLFPVLSPRRDALQAFLRAASIETLIHYPVPIPRQPALEAQSPAACPEADRVC
                     RRILSLPLYPGIPEEDVRAVAEAVRGFRA"
     gene            19113..21632
                     /locus_tag="PLT35_00530"
     CDS             19113..21632
                     /locus_tag="PLT35_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27736.1"
                     /translation="MLQSLAWLAVVAVLPGAVLFRLPVADRPRRAALPADERAFWAVV
                     LSACVTTAMALALAAAGVYSLARVAGADAGLALLLGLTYRRRLAYERPPAAVSPTAAL
                     PLAIAALGAWLYFPASEYVMGGKDPGTYMNEGIQIAQGGSLTIHDATLAGVPDEFRPL
                     FLTVNGDEIRLGLNQGVRFMGFFVKDRSRGQVVGQFPHAFPAWIALAYGLDGLSGARA
                     AVGAWAILGLLAVYFAGARFAGRAPAFIGTLLLAINVAEVWYAKYPNSELMQQALLFA
                     ALLALARAAQDGDRFFAPVAAALLGTLLFVRLDTLVILGGVAAGLLCLIADGKRLGLA
                     FPAVLAAFFGAAAAYYAGPLRAYVAIPLMQMGGVRGLALGLAGFGAAFALTRALRQRR
                     PGLVGALLPWVPRALSAALLAAAAYAYFLREPAGLLAEHDAYALRAFAWYVGPAGLLA
                     AVAGFAAVAWTRFWRDPVLLTAGALVSVFFFYKIRIVPENFWQARRYLSLILPLACLM
                     IASGAFVAYRAHAAPAGRAATRAPVARRAFAWLGAPLAIVACIGAAFASASRPIRRHV
                     EYAGLIPALERLAGGFGDRDLVLVEPRNASDAHVLATPLAYIYARHVLVFASPRPSRT
                     QLELFLSWASHAYDRVFLVADGGFSFASPRIGVTPVGTDRFTVPEYESVRNGYPDEVR
                     HKPFDLYLYELAPLEEPTPTDIDVGSCDNAWVFRMFTRQIQDEVTYRWTRERSFVSLP
                     IDAPTRAIVFRLGDGGRPERAGPAEVQVFLNDHPLGTLAVRGGFADYRVAIPPDIALE
                     AGKGPLSPLVRLVSTTWVPQAVLGTPDDRALGVMVDRIRLE"
     gene            21636..>22858
                     /locus_tag="PLT35_00535"
     CDS             21636..>22858
                     /locus_tag="PLT35_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG27737.1"
                     /translation="MLQVANPWERRAIAAADAALGAAAWIRRRRQPRPWPPPRRILVF
                     RLERIGDLVMSLPALHALRERAPKAFITLAVGSWNLPLALQIAGLDSIETLDAPWLSR
                     GAASAGWRAMVCRARSWRRHGYDLAINLEGDIRSHLLMALTGAARTAGFGMAGGGPLL
                     DECVPFDPRSHTAINGVRLVSAAFGERAAGEWPVEGREAAAALPRATLRIPPSASSEA
                     ARLLADASGTQGGRPLVGLQVGAGRAVKEWPLPRLAEIGAWAHRELGAALVLTGSAAD
                     GDAARALRGWLPPDARVADLTGAGDLLSLAAVLSRLALFVTPDTGPMHLAAVMGTPLV
                     AVFGPSSPERWGPLAAACRIVRIDLPCSPCNRIRNPPARCQGHTPDCLEGIAADQVIA
                     AARDLLDARNTEHADG"
BASE COUNT         2888 a         8547 c         8203 g         3220 t
ORIGIN      
        1 gatgcggcgc atccgctgct cgaggaaagc ggcggcggcg aggatcaggg tgagcttcgc
       61 cagctcggac ggctggatgc cgataccgcc cacgccgaac caccggcgcg tgccgttgat
      121 cttcgggccc acgatcagca cggccaggag cgccacgacg gtgagggcga tgacggccag
      181 tatgacccgc gggttgcggt agcgcgtgta gtccacgtgc atggcggcga acatcgagac
      241 cagccccagc acggcgaaca ggacctgctt cgcgacgaac ggattctcct cgccctgtct
      301 cgccgcgatc gcctcggagg cgctgtagac catgaggatg ctgacggcca cgagcaccag
      361 cgtcgtggcg aacagccaga catcccactt gagctttcgg gccatctcta tagtcccgcc
      421 gtgttcacgc cgacacagag ggcgccggcc gatccgggca cgggcccggg cgtgagccgt
      481 cagcagtcat cagccgggtc ctgggcacgg acaccagctc cccgcgcggg gccgcgcggg
      541 gtcccagcag ccagttccga gccgcgcgga accgactgac gactgctcac ggctcacgcc
      601 ggggtcggcg ctcttccttc ctccgttccc cttcgcgccg cagcgcctcg acttcggcct
      661 tgaaggcccg cccgcgcgcg gcgtagtcct cgaacatgtc gaagctggcg cacgccggcg
      721 ccagcagcac gctccccccg ggcgcggccg cgtcggccgc cgtccgcacg gcgtcggcca
      781 tcgatccggc caggcgcacc gtcgccgcgc cgtccagcgc gtcctgcacg agcggcgcgg
      841 cttcgccgat cgccacgacc gtcgcgcgcc gggcggccag ggcgggccgg agccgcgcca
      901 ggtcgccgcc cttgaagcgg ccgcccatga tgacgacgag gccggggccg aacgtctcga
      961 tggcgtggcg cgccgcgtcc acgttcgtgg ccttcgagtc gttgatgtag cggacgcccc
     1021 cgatgtcggc gacgacctcc atcgcgtgct cgaggcctcg aaacgacgac acggcgccgg
     1081 ccaccgcgcc ggcaggcgca ccggccgcgc gcgcgacggc tgccgcggcc agcacgtcgg
     1141 cgagcaggtg cgggcccacc aggcgcacgt cgccggcccg catcagccgc tcgtcgccgc
     1201 ttcctccgcg ctcgacgatc cagccccccg acacggtgac gcctgcgtcg atccgggcgt
     1261 ctcgcgcgaa gcgcacgacg cgcgcgcggc ccgtctccgc caccgccacg gcgcgggcgt
     1321 cgtcggcgtt gacgacggcg acgtcggcct ccgtctgtcg cgcgaacacg cgcgccttcg
     1381 cggcggcgta ctcctcgaag ctggcgtggc gatcgaggtg gtcctccgtc aggttgagca
     1441 gcgccgcgat ccacgggtgg aacgtgtcgg tcgtctcgag ctggaagctg ctgacctcga
     1501 ccacgtggat cgtcgcgggc gtcgaggagg cgacctgcgt ggcgagcggc gtcccgatgt
     1561 tgccgccgac tggggcgtcg tagcccgcct cggccaggat cctgcccgtc agcgcggtgg
     1621 tcgtggactt gcccttcgtg ccggtaatcg ccacgatccg tccgcgcagc cagcgcgacg
     1681 ccagttcgat ctcaccgatg acggggacgc ccgcgcggcg cgccgcggcg acggccggct
     1741 gcgtgatcgg cacgcccggg ctcaggacga cgaggtccgc gcccgcggcg agcgcggccg
     1801 ggttggagcc gaggacgagg gcgacgccag ccgcctgaag cgccacctgg ctgtcgatcg
     1861 ccgcgcgcac gtcggtcagc gtgaccgacg cgccgcgctc cgcgagcagc cgggcggccg
     1921 ccgttccgct ggcgccgccg ccgaccacga cggcgcgcat cccgcgaacc gagaacccgc
     1981 ctgtcgactg cgggcccgtc acggcgcacc cgccttcagg cggtccgcca ccacgtcggt
     2041 gcggacgccc cgcgatccct tcacgagcac gacgtcgccg ggccgcaccg cacgggcggc
     2101 ggcgtcggcc gcttcgtcgc tggtggcgaa gtgggccacg gcctccgccg ggatcccgcc
     2161 ggtcacggcg gcgtccgcca tggcgcgcgc cggcgcgccg cccacggtca acagctgcgc
     2221 cagccccgcg cgcgccgccg cgtggccggc gcgctcgtgg aaggcgacgg tgtcgacgcc
     2281 aagttcgagc atctcgccga gcacggccag gcgccgctcg cagccgcgct cggccgcgag
     2341 cgcgtccagc gcggcctcca gcgcgcgggg actcgagttg tacgagtcgt ccacgagcgt
     2401 cacgccgccg gccaggcgcc agacctcgcc gcgcctcggc gaggccttca tggcggccac
     2461 gcgggaagcg gcgtcggcga gcggcacccc gtagtgcgac gccacggcga tcgcggccag
     2521 cgcattggcc aggtgcccgc ggccaaacag cggcacgtcg agctcgaatg cgcccgcgcg
     2581 cgtcgtgacc gtggcgcgcg tcccggccac gcccagatcg gccacgtgcg ccgccgagac
     2641 gtcggccggc acgccgacgc cgaaggtcac gacttgcgcc gccgtcgcgc gaacccgggc
     2701 catcaccagc gggtcggccg cgttcgccac cacgagcgac gcgcggtccg cgccctcgag
     2761 gatctcggcc ttggcgtcgg cgatcgcctc gatcgacgcg aagaacgcgg agtggacctc
     2821 ggccaccgtc gtccagacgc gcacgtccgg ctccgccacc tccacgagcg cccggatctc
     2881 gccggcatgg ctcatgccga gctcgacgac ggcgatgtcc gggccgcggc ggagttcgag
     2941 gagcgacagc ggaaggccga tgtggttgtt gaagttaccc ggagcgcgga acacgcggtg
     3001 gccgagggcc agcaggtcgg ccgtcatctc cttcgtggtc gtcttgccca cgctgccggt
     3061 cacggccacc acctgcgccc gcgactcgcg ccggacgtgc tgcgccaggc gctgcagcgc
     3121 gcgcgtggtg tcctcgaccc tgatgacgat cgcgtcccgc tcgaggcggc gcgggacggc
     3181 gccggcggcc gacacgacga cgcccgccgc gcccgcgccg agcgcgtcgg cgacgaagcg
     3241 gtggccgtcg aaccgcttgc cgatgatggc gaagtacagg tcgccggcgc gcagcgtcct
     3301 cgtgtcgatc gagaacgcgt ccagcggggc gccagccggt cctgcggcca gcacgccgcc
     3361 catggccttc gccgcctggg ccgccgtcag cgtcaccgcg gcgctcacgg cgtgctccgg
     3421 aggcggcgcc ggtcgagcgc gtcgcgcgcg acggcggcgt cgtcgaacgg cagcgcccgg
     3481 tcgccgatca cctggttctt ctcgtgcccc ttgcccgcca ccaccacgac gtcgcccgcg
     3541 tcggccccct cgatggcccg gccgatcgcg gcccggcggt cggccaggcg cagcctgggg
     3601 cggtgcggcg ggatgccccg ctcgatgtcg tcgagaatgc gctcgggatc ctccgatcgc
     3661 gggttgtcgg aggtgaccac caccaggtcg ctgaggcgcg cggcgacgtc ccccatcagc
     3721 ggccgcttcg cgcggtcccg gtccccgccg cagccgaaca ccgtcgtcag ccgcgcgccc
     3781 gccatcgcgc gcaccgcctc caggacgttc ctgagggcgt cgtcggtgtg cgcgtagtcc
     3841 acgatcaccg tcacgtcgtc ggcggccgac gagacgacct ggaaccggcc cggcaccgag
     3901 gccaccgagg acagtccggc ttcgatcgcc tgcgcgggca ggtccagcgc gacgccgatc
     3961 gcggcggcgg cgagcaggtt ggagaggttg aaccggccgg cgaggggcga cgagatgcgc
     4021 agcgtcacgc cgcgggcggc cacgctgaag tcgagcgcgg cgagggaggc gggcatggcc
     4081 gcggggcgga cgtcggcggg ggcgtcgacg gcgaaggtga cgcaggggcg ccgcgaggcg
     4141 gcgagcgacg ccccgcgccg gtcgtccacg ttcacgatgg agggcgcgcc gtccggcagc
     4201 agatcgaaga gccggcgctt cgccgcgaag taccgatcca tgtcgccgtg gaagtcgagg
     4261 tggtcccgcg tcaggttcgt gaaggcggcg gcggcgaatt cggtgccgtc cgcccgcttc
     4321 agcgccagcg cgtgcgacga cacctccatc acgcacgcgc cgcagccggc cccggccatc
     4381 tcgcggagca ggcgctgcaa ttccggggcc tcgggcgtcg tccgaaccgc gtcctgtgcc
     4441 tcggctccga cccggtagtg caccgtgccg agcaggccgc agcggatgcc ggctgcctcg
     4501 aagaccgcct gcgccagaaa cgaggtcgtg gtcttgccgt tggtgcccgt gatgccgacc
     4561 acgcgcatcg accggctggg gtggccgtgc acgctcgcgg ccagcagcgc cagggccagg
     4621 cgggcgtcgg tgacccgcac ccacggcacg ggaaactgcg gcggcgcgtc cgcctccgac
     4681 acgacggcgc acgcgcccgt cgaggcggcc tgcgccgcga aggcggcccc gtcgacgcgc
     4741 tggccgcgca gcgccacgaa caccgcgccc ggcgacacgg cgcgcgagtc gcacaccacg
     4801 tcgagaaccg ggacgcctcc cgccggcgtc tcggcgccgc ctgcgcccgg tgcgcgaagg
     4861 ccgggcccgt ccagcacggt gaggaggtcc cggagcgtca tggggcgggc gccggagcag
     4921 gaggcaggga agagaggcgg ccgagccaca cgcggcacac gcggccgggt tcgagcacgg
     4981 agccggcggc gggatcctgg gcgatgacga cgccgtcgcc cgtgagccgc acgaggagcc
     5041 cgaggcgcga caggcggcgg gcggcctccc tcgccccgag accgcgcacg tccggcaccg
     5101 tgggcggcgc ggtcagcacg ctgatgcgag gcgggccggc cgtcgcggag gcctggacca
     5161 actccaccgg cggaggatcc tccagcgacg ccttcacgag taccggcggc agcgggttga
     5221 ttgtcggcgg cacgccggcg tagcgcagcg ccgcgtccgc gatccgctgg aagacgggcg
     5281 ccgcgaccgc cccgccgtag atcgccccgg cgtgcggcga gtcgatgacc accaggattg
     5341 tgaaagccgg ccgccgggac ggcacgaagc cgacgaacga ggactggtag tccgtcttcg
     5401 agtacccccg ggcgacggcc ttgctcgccg tgccggtctt gccggccacc gagtagccgg
     5461 ccacccgcgc ggcggtcccg gtgccgcgct ccacgacctg ctccatgatg gacacgatct
     5521 cggaggcggt cgcctcggag atcgggcggt gcagctcctg cggcatcacc ccggtgcgca
     5581 cgccgccccg cctcagcgcg cggacgatgc gcggtcgcag caggacgccg ccgttggcca
     5641 ccgccgacac cgccgcggcc atctggaggg gcgtcacgcc gatctggtag cccatcgaga
     5701 tggacatgag gccgctctgg ttccactggg ccgaatccag caggaggccc cgcgtctctc
     5761 ccggcagatc gaccgagagg cgctcgccga agccgaaccg gcgcgcatag cggcccaggc
     5821 gctcggcgcc cacgccgagg ccgatcttga tcgcgcccac gttgctcgac ttcacgatca
     5881 cgtcggtgaa gctgagcacg ccgtaccggt gcgtgtcgga cacgacccgt ccagcgaggc
     5941 ggatctgacc cgcgctcacg tcgaacgcgt cggtcggccg gaacgtccgc tcctccatgg
     6001 ccgccgacgc ggtcacgatc ttgaacgtcg agccgggctc gtagatggcc tggacggcgc
     6061 ggttcatcca cgcctcgcgc gtgtagtccc ggtacgcgtt cgggttgacc gcgggcgcgt
     6121 tcgccatcgc gaggatctcc ccggtccggg gggccatgac gatgaccgtc ccgcccagcg
     6181 cgcggtgctc cgcgacgccg cgctccagct cccgctcggc gatgtgctgg aggtacgcgt
     6241 cgatggtgag ttcgatcgtg tcgcccggca ccggggggat ctcgacgcgg ctgaacgcgc
     6301 tcttgttcgc atcgaccttg acgatgatct ggccggccgt tcccctgatc ctcgactcat
     6361 acgactgctc gatgccggcc aggccctggt tgtcggtgcc gaggtggccg agcacgtggg
     6421 ccgcgagctc gcggttcggg tagtagcgcc ggggctcggc cttcatgaag acgccctgca
     6481 agccgaggcg ctccacctgc gcggccgctt cgatcgaggc ctggcgccgc aggaacgcga
     6541 acgcgcggtc cttctggccg agtcgctgcg cgaagagccg ccgctcctcc gcggtgcagt
     6601 cgccgagggt ggcgcagact gcggccacga cggcaccggg atcgtcgatc tccttcggga
     6661 tcgcgcagat ggaggtggcg tccacgctcg tcgccagcag gcgcccggct cgatccacga
     6721 tgtcgccgcg cctggcatcg gcggcgaggg tccggttccg ctgtccggcc gcggccgcct
     6781 ccagcgcgtc ccgctggaac acctggaggt ggacgaggcg ggcctcgatc gcccccgccc
     6841 acagggcgag gcacgcggcc atgacgagca cgcgcgcgcg catcgccgcc agccacgccc
     6901 gcccgaccgg ctcattcatg gccgcccgct ccgccctccg gcgcctgccg gcgcgccacg
     6961 agcgtgcgcg ccggcgcggc ggcctgcctg acgcgctcga gcacgacggc ttccgtctgc
     7021 gacggcgcga ccaggttcag gtcctggatc gcaaggcgct caatccgctc cggcgcgcgc
     7081 agcgtctcga gctccagcct gaggtggcgc tgctccgcct cgcccgccgc gcgcgccttc
     7141 tcgagccgcg caaggtcgta gccgagggcc agccactccg agtgcaggcc ggccaccacg
     7201 agcaggccga cgagcagcag cgccacgacg ccggccagcc gcagcatgtc gcgccggcgg
     7261 gcgcggtcca ccacgcgcac cgggtggttc tcgacctccc gggggatgct gtaggcgatg
     7321 gcgtccgccg gttcgtgtct gttcgccatc acgtcactcc atccgctcaa tcacgcggag
     7381 ccgcgcgctg cgcgcgcgcg ggttcgcccg ccgctcctcc gccccgggga ccacggcctt
     7441 cttcgtgacg agccgcagcg ggccggactc gcccgcaagg gcccggagcg tgtgcttcac
     7501 gggccggtcc tcgagcgagt ggaacgtgat cacccccagg cgcgcgccgg cgcccagccg
     7561 gccgcacgcc gcgcggacga acgcgtccag cccgtccagt tcgccgttga cccagatccg
     7621 gatcgcctgg aacgtccgcg tggccggatc gatccggccg gatcgcccca cgacgcggcg
     7681 cacgatggcg gcaagccgcg ccgtcgtgtc cacgggtgcg gtgcgccggg cctcgacaat
     7741 cgcgtgcgcg atcgcccgcg accggcgttc ctcgccgaag cggaagatca cgtccgagag
     7801 cacctgctcc ccggcggagg ccaccaggtc ggccgccgtc gggccccggc tggtatccat
     7861 ccgcatgtcg agcgcctcgt ccctcctgaa actgaagccc cgccccgcgc cgtcgagctg
     7921 cagcgacgac acacccaggt ccgcgacggc gccgtccacc gtcgtcaccc cgcgctcgtc
     7981 gagcagccgc gcgaggtcgc ggtagtcgcc gtgcacgagc gccacccggt cgccccattc
     8041 cgccagcgcc tcgcgggcgc ggtcgagcgc ctcgccgtcg cggtcgatcc cgacaaggcg
     8101 cgtggcgccg gcggccagca gggcgcgggc gtggccgccg gcgcccacgg tgcagtccac
     8161 gaagagcccg cctcgcgccg gccgcagcgc ctccacgacc tcggcggcca tgacgggcac
     8221 gtgaacgggc atggcggcgg caccccgggg gcgtcagatc ccgaagtcgg cgagcgcgcg
     8281 cgcgtcggcg tcggtgtacg gatcgttcgc gagcttggtg acgaaccgct cgtggttcca
     8341 cacctcgagg tggtcgtact gcccgaagac gtccacctcg cccgccatcc cggccgcttc
     8401 ccggagccgc ggatgaatca ggacgcggcc ctggttgtcg agctcggccg tctgtccgta
     8461 gtagttgacg cggctgaaga agcggaggcg tgacggatgg gtggacggga tctgcgccag
     8521 cttgcgttcg atctcgatcc acacgggcat cgggtagatg cggacggact cgccgctcag
     8581 gctggtgacg aacagatccc ggccgtgccg ggcctcgacc agggccttga aggcggtggg
     8641 aatcttgagc cggcctttgt catcgatgcg cgccggcgcg ttccctctga gaacggcggt
     8701 ctgctccact ttgctccacc cgagaccact cggatgttag agaagcggga gggggatgtc
     8761 aacgccaaaa acctgttccg gcgcaggtta cggcgattcg ccgttcgctt ttgcttcgcc
     8821 ggtcggcccg gttcggcccc ttcctgcagc gctccgcgcc atccgggcgc gtgctagcat
     8881 ggggctcagc cgccaggcga gccgccgccc cggctggccg gacgatccgc ccatgctccg
     8941 aaccgcgttg atcggcttcc cctcgaccgg caagacggct ctgttccagc tcatgacgtc
     9001 cgcccacggc gcgccgcggg ccgcccacgg cagaacggac gtgacgctcg gcgtggcgcg
     9061 cgtgccggac ccgaggctcg acgtgctgac cgcgatgtac cgcccccgga agcgggtgcc
     9121 ggcgatggtg gagttcgccg acattcccgg gcggccgtcc ggcagcggcg ccggcgccct
     9181 gctggatgtg gccgcgtacc ggaacgccga cgcgctcgtc cacgtcgtgc gcgcgttcga
     9241 ggacccggcg gtcccccacg cagccgggtc gatcgatccg ctccgcgacg cgcgggcgat
     9301 ggaagacgag ctgatcctgg cggacctggc ggtggcggag aagcggctcg atcggctggc
     9361 gaaggatctc aagaaggcgc gaacggccga gctcgagggc gagcaggcgg tcctcgcggc
     9421 gtgccgcgcc cacctcgagc gagggacggc cctgcgcgat ctggagctgc ccgacgacga
     9481 ccggcgcagg ctgcgcggct tccagttcct gtcggccaaa ccgctcctgc tggttctgaa
     9541 cgccgacgag cgcgatctgg cgcggggact cgaccgtttg cccgagcggt acggtctcgc
     9601 cggcccggga acggggccgc gcacccgcgc cgtcgcggtg tgcgcgaaga tcgagctgga
     9661 agtcgcgcag ctcgacgagg ccgacgccgc ggccttcctg gcggacctcg ggctgaaggc
     9721 ctctgggctc gaccgggtga tccgcgccag ctacgatctg ctggggtacg tctcgttctt
     9781 caccgtcggc gaggacgagt gccgcgcgtg gtcggttccg cgcggaacgc cggcgcaggc
     9841 cgcggcgggc gagatccaca cggacctgtc ccgcgggttc atccgcgccg aggtggtggc
     9901 gttcgaccgg ctcgccgggc gcggctcgat ggcggcgtgc cgcgatcatg gcgaggtgcg
     9961 cctcgagggc aaggactacc cggtcgcgga cggcgacgtc atcaccgtcc gattcgcgac
    10021 gtgatacggt acccggggcc gcggcggcgg cgatcgcgcg cggcgcccgc ctggtgagca
    10081 ggatgccggt ccggaccata ctcgtgtgcg aggcgcaggt gccgtttgtc aggggcggcg
    10141 ccgagagcca cgtgcgccag ctcgtcgatc gcctgcgcgc ccgcggctac gccgtcgacc
    10201 tcgtgtcggt gccgttcaag tggtacccga aggaggagat cctcgcgcac gcggcggcgt
    10261 ggcggctgct cgatctcagc gagagcaacg gcacgcccgt cgacctggtc atcgccacga
    10321 agttcccgtc gtacttcgcg cgccatccga acaaggtcgc ctggctcatc catcagtacc
    10381 gcgcggccta cgagctgtgc ggcaccgtct acagcgactt cgcccacgcg gatctcgacg
    10441 cgggcctgcg ccagtcgctg ctggatctcg acacgcggat gctcggcgag tgccggcggc
    10501 tctttgcaaa cgccgggaac accgcgcggc ggctcgagaa gttcaacggg ctcacggccg
    10561 aaccgctgta tcatcccccg agggtcgccg gccgcctcgc gccggggccg tcgggcgatt
    10621 acgtgctctc ggtgggccgc ctcgaaacgg tcaagcggca cgaccttgcg atccgggccc
    10681 tggcgcacgc gcccccccgc ctgcggctgg tcgtcgcggg cgagggcacc caggacgcga
    10741 acctgcgccg cctggcggag tcgtgcggcg tcgccggccg cgtcgccttc gagggagcgg
    10801 tcgacgacgc gcgcctgctg gcgctctacg ccggggccct cgcggtcgtg tacgcgccgt
    10861 tcgacgagga cttcggctac gtgacccttg aagcgtttct ctcccacaag ccggtcgtca
    10921 cggcccgaga ctcggggggg ccgctcgagt tcgtcgaaga cggcacgaac ggctgcgtgt
    10981 gcgatccgga cccggccgca gtgggcggcg cgttcgcgcg gctggacgcg gaccgcgagc
    11041 gcgcccgggc cctcggcgac gcggggttcg agcgcgcgcg gctgatcacc tgggatgggg
    11101 tgattgagag gctggttggg gaataggttg ctgcccgatg aaactgatcg tccagatccc
    11161 gtgcctgaac gaggcgcaga cgctgccggc gacgctccgg gacatcccgc gcgccatccc
    11221 gggcatcgac gtcgtcgagg tgctcgtcat cgacgacggc tcgcgcgacg gcacggcgga
    11281 ggtggcgcga cggcacggcg tcgagcacgt cgtgcggttc ccgcagcgga agggcctcgc
    11341 ctcggccttc gccgcgggaa tcgacgcctg cctggcgctc ggcgccgacg tgatcgtcaa
    11401 caccgacggc gacaaccagt acgacggggc cgacatcccg aagctcatcg ccccgctcgt
    11461 cgcgggcgag gcggacatcg tcatcggcga tcgcgaggtg cgcgacctgg cgcacatgtc
    11521 ggccacgcgc aagcggctgc agtggctcgg ctcctgggtc gtccgccagg tgtcggacac
    11581 gaaggtgccc gacacgacga gcggcttccg cgcctacacg cgcgaggccg cgatgcggat
    11641 gacgatcatc tcggagttca cctacacgct ggagtcgatc atccaggccg gcaagcggcg
    11701 gatggcgatc gtgcacgttc cgattcgcag caacccgggc acgcggccgt cgcgcctgtt
    11761 ctcgaacgtc tggacgtaca tcaaggcgtc ggcggcgacg atcatccgcg tctatgcgat
    11821 gtacgagccg ctcaaggtgt tcgggctcat cggcctggtc gtcttcggcg gcggcatcgc
    11881 cgtctcgctg cgcttcgtgt acttctacct ggtcggcgag ggcctcgggc acgtccagtc
    11941 gctcatcctg gcggcggtcc tcctgatcgt cggcttccag atcggcctca tcgggctgat
    12001 ggccgacgtc atctcgggca accggaagat gctcgaggac ctgatctacc gcgtgaggcg
    12061 gatggagctc cgccagaacg gggccgcggg cggtccgccg ccgggcccgg accggagtta
    12121 gggccgtggc cgagccgtca tcggtctcga ttgtgatccc ggcgttcaac gaggagcccg
    12181 cgatcgggcc gctcgtcgcg gcgctcggcg ccggcgccgc ctggcgggag atcatcgtcg
    12241 tcgacgacgg gtcctcggac gcgacggccg cgcgggcccg cgaggccggg gccaccgtgg
    12301 tgcgccaccc ctacaacaag ggcaacggcg cggccgtcaa gaccggcatc cgcgccgcga
    12361 cgggcgagta cgtgctcgtc gtggacggcg acgggcagca tcgcgcggcg gatgcggcgc
    12421 gcctcgtcgc gcgcctcggc gagtacgacc tcgtgatcgg cgcgcgctcg tcggacacgc
    12481 aggccacgca cgcgcggctg ctggggaacg ctgcgctgaa ctggctggcc ggctccctca
    12541 cggggcgcgc gatcccggac ttgacctccg gcttcagggg cgcgcgccgg acgcacctgc
    12601 gcgagttcct gcacctgctc ccgaacgggt tctccacccc gacgacaacg acgctggcct
    12661 tcatcaaagc gggctacaac gtctgcttcg agcccgtcga ggccgcagcg cgcgtcggct
    12721 cctcgaagat ccgcctcgcc cgcgacggcg tccggttcct cctgatcgtg ttgaagatcg
    12781 tgacgctctt cagcccgttc cgcgtcttcc tcccgatcag tctcgccgcc tttgccgccg
    12841 gggccgggta cgcggcgtgg accgttgcca cgcagtcgca cgtgacgaac tcctcggtgc
    12901 tgctgatcat gctggccgtc atcgtgttcc tggtcggcct ggtctcggag cagatcgcgg
    12961 cgctcaggtt cgaaggccga gagtagcggc cgtgagcgca cagatgcccc gcgcggtgtg
    13021 gatggcggtg gcggcgggcg tcgccctgcg cctcgctttc gccctcggct actgggtgga
    13081 caagcccctc acgcacgacg agcgcgagta cctgacgctc gccgggaacg tcgcggccgg
    13141 gcgcgggttc gtgcacctgt ctcccgacgg atcttccgtc ccgggcgagc acttcggccg
    13201 ggcccccctt tacccgctgt ttctcgccgg cgtggtgcgc gcctccggcg ccgagggcgc
    13261 ttccgccatc ccgccggccc ccgacgcggc gcccgcggcg gcggttctgc gcgccgtgcg
    13321 ggccgcgcag tccgttctcg gaggggcaat cgtctggctg gtcgcctggc tggccggccg
    13381 cgcggccggc ccgcgcgccg ccgcctccgc cgccgcgctc gccgcggtgt acccgccgct
    13441 tgtctggacg ccggcctacg tctggagcga aacgctgttc agcgtgctgg cgctcggctg
    13501 cgccgcggtc ctcacgcgca cgccggggag ccctggcgga gcggccgctt ccggcgccct
    13561 cggcggactg tcggtgctga cccggcccgc gatgctgttc ttcctgccgc tcgccttcat
    13621 ctggctcggc tggcgccacg gctggcgcgc tgccgcgatc ctgacggtgg cctgcctcct
    13681 cgtcgtcgcg ccgtggacga tccggaacgc gcgcgagtac gggcggttcg tcctgatcgc
    13741 ctccgaggga ggcatcacgt tctggaccgg caaccacccg ctggccgtgg gcgagggcga
    13801 tctcgccgcc aaccccgcgc tcaagcgcgc caacgctgac ctgcgtgcgc gctacccggg
    13861 ccgcagcccc gaggagctgg agccgatcta ctaccgggag gcctggcgct tcgtcggcga
    13921 agcgcccggc gcctggctgc gcctcgtcgc gcgcaaggcg ttctacacgg tcgtccccgt
    13981 cgggccgtcc taccgcctgc attcggcgcg ctactacgcg gcgtcggccc tgtcgtacgc
    14041 ggcggtgctg ccgttcgcgc tcctgggcct tcgcgcgctc tggcggaaag ggcggctccc
    14101 ggcgccgctg ctgttgctcg catcgtcgtc gctcgccgtg tgcctggtgt tcttcccgca
    14161 ggagcggttc cggatcccgg tggtggaccc gacgctcctc gtcgcggcgg catcctggcg
    14221 agcgctgcgc gggtgagggc atgaacgtcc tggtgatcgt cccgacgtac aacgagcgcg
    14281 agaacctgcc gctgctcgcg cgcgaggtcc tggatcggca gccctacgag atgctcgtcg
    14341 tcgacgacga ctcgccggac ggaactggcg cggtcgccga cggcctcgcg cgcgactacc
    14401 cggggcggct gcacgtgatg caccgggcgg gcgcgcgcgg cctcggcctg tcgtacctcg
    14461 acggattcag gtgggcgctc gcctcgggcg ccgacttgat ctgccagatg gacgcggacc
    14521 tgtcgcacga cccgcgctac ctccccgacc tggtggccgc ggcgtcggcg ttcgacctgg
    14581 tgctcggctc gcgctacctg cacggcatca gcgtcgtgaa ctggccgctg cggcggatcg
    14641 tgctcagctc gttcgcgaac gcgtacgtcc ggacgatcac ggggctgacg acgcgcgaca
    14701 gcacgagcgg gtaccggtgc tggcgccgcg gcgcgctggc ccggctggat ctcgagcggt
    14761 tcctctcgca ccgctacgcg ttcatggtcg aaaccgtctt cgaggtggcg cgggcgggca
    14821 tgcgcgtcgg cgaggtgccg atcatcttcg tcgagcgccg gcgcggcgcg tcgaagatgt
    14881 cggcgcgcgt gctggcggag tcgatgatca tgccgtggcg gctcgtgctg cggaaccgcg
    14941 gacgggtgcg cccggcgccg ggaggcgcgg ccccatgagc cgccgccatg tcgtcgtcat
    15001 gctgacgacg tcctacccgc ggttcccggg cgacgcgatc gcgaccttca tggagccgat
    15061 cgccctgggg ctagccgccc gcggccacca ggtgcacatc gtgctcccgt ggcacccgcg
    15121 cctcgcccgc ggacccgccg agggcggcgt gcgttttcat ccgttccgct atgcgccgca
    15181 cccctcgctg aacgtgttcg gctacgcggc ggcgctgcgc gcggacgtcg agctgcgcgc
    15241 cgcggcgtac gccgcggcgc ccctcgcgct cgccgcctcg ttccgcgccg cccggcgcgt
    15301 cgcgcgcgag gccggggcga cgatcctcca cggacactgg ctcgtgccga gcggcgcggt
    15361 ggcggcggcc gccgccggcc ggctgcccgt cgtcatcagc ctccacgggt cggacgtgtt
    15421 cgtcgccgag cggcacgccg tggcgcgccg cgcggcgcgc gcggcgctcg gccgcgccga
    15481 cgccctgacg gcctgcagcg acgatctgcg cgaccgcgcc atcgccatcg gcgcagaccc
    15541 cgctcgctct gagacgatcc cctacggcgt ggacgtggag cgcttccgcc cggatgccgc
    15601 gcgcagggcg gagacgaggc gggccatggg cgcagacgcc gacgacccgg tgctcttcac
    15661 ggccggccgc ctggtccgga agaaggggtt cggcgacttg atcgacgccg tgggcctgct
    15721 cgcgacgcgc tggcccaggc tgcgcctcgt catcggcggc ggcggggatc tcgacgcgga
    15781 gctgcgcgaa cgcgcccggc gtctcggcgt cgccgatcgc gtggagttcg ccggagtcct
    15841 ggcccaagac gaggtcgccg cgcggctggc ggccgccgac gtcgccgtcg tgccgtccgt
    15901 ccacgacgag gccggcaatg tggacggcct gcccaacacc gtcatggagg cgctcgcctc
    15961 ggggacgccc cttgtggcga cccgggtcgg cgggatcccg tccgtcgtgg cgcacggccg
    16021 gacgggctgg ctcgtgcccg agcgcgatgc ctcggccctc gcgaacgccc tcgatcgcct
    16081 gctggccgac ccggcctccc gagccgggct cggcgcggcc gcccgcgccg aggccctcgc
    16141 cagccacacc tgggcgcgcg tcgccgagcg tttcgaggcc gtctacgacc gcgcggcttg
    16201 ccgggccgaa gaacgccgtg gtacggttta ggcgctcgag atggctgacc agcaggcgac
    16261 acccgggctg acggtgttct tcccggccta caacgacgcg ggcaccatcg ccagcatggt
    16321 ggtgaccgcc gtgctggccg cctcgcgcct cacgcccgac gtcgagatcc tcgtcatcaa
    16381 cgacggcagc cgggacagga cgccggcgat cctcgacgaa ctggcgcgcg tctacccgca
    16441 ggtccgcgtc atccaccacc cgacgaaccg ggggtacggc ggcgcgctgc gcagcgggtt
    16501 cgcccatgcc tcgaaggact ggatcttcta caccgacggc gacgcgcagt acgacccctg
    16561 ggagatgggc gtcctctggg cggcgatggg gccgggcatc gacatggtga acggctacaa
    16621 gatcagccgc tctgatcccc tccaccggat cgtcatcggc cgcctgtacc atcacatggt
    16681 gaagcggctc ttcgggctga aggtccgcga cgtggactgc gacttccgcc tgatgcggcg
    16741 cacgatcttc gaccgggtga cgctcacgaa ggacagcggc gtgatctgcc tggagatgat
    16801 gaagaagatc caggacgcgg ggttcacgat cgccgaatcg cccgtgcacc acttccaccg
    16861 ggcgtacggc aagtcccagt tcttcaacct caggcgcatc gcccgcacgg ccgcggacgt
    16921 gctgaagctc tggtgggctc tcgtcgtccg ccgcagcgcc cagcccccgc cggcgcccgc
    16981 ccgctccgga ggctgaggat ggagtacgag gacttctacc gcggccgccg cgtgatgatc
    17041 accggcggcg cggggttcat cggcagcaac ctcgcgcgcc gcctggtgga cctcggcgcc
    17101 aacgtgctgc tggtcgattc gctcatcccc gagtacggcg gcaatctctt caacatccgc
    17161 gggatcgcgg atcgcgtgcg gctgaacgtc gccgacgtgc gccaggacag cacgatgagc
    17221 tacctcgtgc ggcacgtcga gatcatcttc aacctcgccg gccaggtcag ccacatcgac
    17281 agcatgcggg acccgttcac ggacctcgag atcaactgcc ggagccagtt gtcgattctc
    17341 gaggcctgcc gccgcaacaa ccccgcggcg aaggtcgtgt tcgccggaac gcgccaggtg
    17401 tacggcaagc cggactgcct gccggtgaca gagcgccatc tcgtgcgccc gacggacgtg
    17461 aacgggatca acaaggtggc gggcgagtac taccacctcg tgtacgacca cgtcttcggc
    17521 gtgcgcgcgt gctcgctgcg cctgacgaac atctacgggc cccggcagct cgtgaagcac
    17581 gatcgccagg gcttcatcgg ctggttcatc cgcctcgccg tcgaggaccg cgagatccag
    17641 atctacggcg acgggtcgca gctgcgcgat ttcgtgtacg tggacgacgc ggcggacgcc
    17701 ttcctgcggg cgggagcggc cgacgcgtgc aacggcgagg tgttcaacgt gggcggcgcc
    17761 gagccggtca gccaccggga cctggtgcgc ctgctgatcg aggtggcggg caccggacgc
    17821 gtgcgcttcg tgccgtggcc cgaggagaag cgggcaatcg acatcggcag cttctatgcc
    17881 gactcgtcga agttcaaggc cgccaccggc tgggagccgc gggtcgctct ccgagacggg
    17941 ctggcgcgca cggtggccta ctaccgcgcg cacctgcccc actacctgaa tcccgaggcc
    18001 caggagctcc ttccgccatg accctgcgga tcccgagcgt ccgcctcgct cccggcgagg
    18061 atcgtcctgc ggtcgaagcg gccgtccgcc gcgtcgtggc cagcggctgg tacgtgctcg
    18121 ggccggaggt cgagtcgttc gaacgggcgt tcgccggcgc ctgcggggcc gccgcctgca
    18181 tcggcgtcgg cacggggacc gacgccatcg cgatcagcct ccgcgccctc ggcatcggcc
    18241 cgggcgacga ggtcatcacg acgccgctct ccgccgccta caccgccctc gcctgcatga
    18301 tggtcggcgc gaggccggtc ttcgccgaca tcgatcctcg gcggctgacg atcgacccgg
    18361 ccgcgatcga atcggccatc acgccgcgca ccgccgcgat cctgccggtg cacctctacg
    18421 ggcagccggc cgacatgcgc gcgatcgcgg ccatcgccgc gcgtcacggc ctcgcgatcg
    18481 tggaggactg ctgccaggcc cacctggcga cgtgcgacgg gcagcgcatc ggttcgttcg
    18541 gcgcggcggc ggcgtacagc ttctacccga ccaagaacct cggcgcgctc ggcgacggcg
    18601 gcgccatcac gaccggcgac gaggccctcg cggcgaagat gcggcgcatc cgcaacggcg
    18661 gccagaccga ccgctaccgc cacgccgaga tgggcgtgaa cagccgcctc gacgagctgc
    18721 aggccgccat cctcgcggcg cgcctgccgt tcctcgccgc gtggaccgcc gcgcgccggg
    18781 cccgcgcggc cgagtaccgg cggctgctgg cgggatcggc ggtcgacgtg ccgccggagc
    18841 tggatgccgg tcacgtctac cacctgttcc ccgtgctgtc gccacggcga gacgccctgc
    18901 aggcgttcct gcgcgccgcc tcgatcgaga cgctgatcca ctaccccgtg ccgatcccgc
    18961 gccagccggc cctggaggcc cagtcgccag ccgcatgtcc cgaggcggac cgcgtgtgcc
    19021 gccggatcct ctcgctcccg ctctaccccg gcatcccgga agaggacgtg cgcgcagtgg
    19081 cggaggccgt ccgggggttc cgcgcctgac acatgctgca gtccctcgcc tggctcgccg
    19141 tcgtcgccgt gctgcccgga gccgtgctgt ttcggctgcc cgtggccgac cgaccgcgcc
    19201 gcgcggcgct gccggcagac gaacgcgcgt tctgggcggt cgtcctgagc gcgtgcgtga
    19261 ccaccgccat ggcgctcgct ctcgcagcgg ccggcgtcta cagcctcgcg cgcgtcgcgg
    19321 gcgccgatgc ggggctcgcg ctgctcctcg gactcaccta tcggcggcgc ctcgcgtacg
    19381 aacggccacc ggcagcggtc tcgccgactg ccgcgctgcc gctggcgatc gccgccctcg
    19441 gcgcctggct gtacttcccg gcgtccgagt acgtcatggg cggcaaggac ccgggcactt
    19501 acatgaacga ggggatccag atcgcccaag gcggctcgct gacgatccac gacgcgacgc
    19561 tcgccggcgt gcccgacgag ttccggccgc tgttcctcac cgtcaacggc gacgagatca
    19621 gactgggact caaccaaggc gtgcggttca tgggcttctt cgtgaaagat cgctcgcggg
    19681 gccaggtcgt cggccagttc ccgcacgcct tccccgcgtg gatcgccctc gcctacggcc
    19741 tcgacgggct cagcggcgcc cgcgcggccg tcggcgcatg ggccatcctg ggcctgctcg
    19801 ccgtctactt cgccggcgca cgattcgcgg ggcgcgcgcc ggcgttcatc ggcacgctgc
    19861 tcctggcgat caacgtcgcc gaggtctggt acgcgaagta cccgaactcg gagctcatgc
    19921 agcaggcgct gctcttcgcg gcgcttctcg cgctggcgcg cgccgcccag gatggcgacc
    19981 gcttcttcgc ccccgtggcc gccgcgctgc tcggcacgct cctgttcgtg cgcctcgaca
    20041 cgctcgtgat cctcggcggc gtggccgcgg gcctgctatg cctgatcgcc gacgggaagc
    20101 gtctgggcct ggcgtttccg gcggtcctgg cggcgttctt cggtgccgcg gcggcgtact
    20161 acgcggggcc gctgcgcgcc tacgtcgcca tcccgctgat gcagatgggc ggcgtgcgcg
    20221 gcctcgccct cggcctggcg ggtttcggcg ccgcgttcgc actgacgcgc gccctgcggc
    20281 agcgccgccc gggcctggtc ggcgccctgc tgccgtgggt gccgcgcgcc ctgtcggcgg
    20341 cgctgctcgc cgccgccgcg tacgcgtact tcctgcgcga gcccgccggc ctgctcgccg
    20401 aacacgatgc gtacgcgctg cgggccttcg cctggtacgt cgggccggcc ggcctgctcg
    20461 ccgccgtggc ggggttcgcc gcggtggcct ggacgaggtt ctggcgggac ccggttctcc
    20521 tgacggccgg cgccctcgtc tcggtgttct tcttttacaa gatccgaatc gtgccggaga
    20581 acttctggca ggcacgccgc tacctgtcgc tcatcctgcc gcttgcctgc ctgatgatcg
    20641 cctcgggcgc cttcgtcgcg taccgcgcgc acgccgcgcc ggcaggaagg gcggccacgc
    20701 gcgcgccggt cgccaggcgg gcgttcgcct ggctcggcgc gccgctcgcc atcgtcgcgt
    20761 gcatcggcgc cgccttcgcc tcggcctccc ggcccatccg ccgccacgtg gagtacgcgg
    20821 ggctcatccc ggcgctggag cggctcgccg gcgggttcgg cgatcgggac ctcgtgctgg
    20881 tcgagccgcg caacgcctcc gacgcccacg tgctggcgac gccgctcgcc tacatctacg
    20941 cccgccacgt cctcgtcttc gcgtcgcccc gcccgagccg cacgcagctg gagctgttcc
    21001 tctcgtgggc gtcgcacgcc tacgaccgcg tgtttctcgt cgccgacggc ggcttcagct
    21061 tcgcctcgcc gcggattggc gtgacgcccg tcggcaccga ccgcttcacg gtgcccgagt
    21121 acgagtcggt gcgcaacggc taccccgacg aagtccgcca caagccgttc gacctgtacc
    21181 tgtacgaact ggcgcctctc gaagagccga cgcccaccga catcgacgtc ggcagctgcg
    21241 acaacgcatg ggtcttccgg atgttcacgc gccagatcca ggacgaggtg acctaccgct
    21301 ggacgcggga gcgctcgttc gtgtccttgc caatcgacgc cccgacgcgc gccatcgtct
    21361 ttcgcctggg cgacggagga aggcccgagc gggcgggccc ggccgaggtg caggtcttcc
    21421 tcaacgacca tccgctcgga accctcgccg tgaggggcgg ctttgccgac taccgcgtcg
    21481 cgatcccgcc ggacatcgcc ctcgaggcag gcaaggggcc gctgtcgccg ctcgtccggc
    21541 tcgtcagcac gacgtgggtc ccgcaggccg tgctgggcac gcccgacgac cgggcgctcg
    21601 gcgtgatggt cgatcggatc cggctggagt agggcgtgct gcaggtcgcc aacccgtggg
    21661 agcggcgcgc aatcgcggca gccgatgcgg cgcttggcgc cgccgcgtgg atccgccggc
    21721 gccggcagcc ccgcccgtgg ccgccgcccc ggcgcatcct ggtcttccgc ctggagcgga
    21781 ttggcgacct cgtgatgtcg ctgcccgccc tgcacgcgct gcgcgaacgc gcgccgaagg
    21841 ccttcatcac gctggccgtg ggaagctgga acctgccgct cgccctccag atcgccggac
    21901 tggacagcat cgagaccctg gatgccccgt ggctgtcgcg aggggcggcc tccgccggct
    21961 ggcgcgccat ggtttgccgg gcgcggagct ggcggcggca cggctacgat ctcgcgatca
    22021 acctcgaagg cgacatccgc agtcacctgc tgatggcgct gaccggcgcc gcgcgcacag
    22081 cgggattcgg catggccggc ggcggcccgc tgctcgacga gtgcgtgccg ttcgatccgc
    22141 ggtcgcacac ggcgatcaac ggcgtccgcc tggtgtcggc ggctttcggc gagcgcgccg
    22201 cgggcgagtg gccggtcgaa ggccgcgaag cggcggcggc cctgcctcgc gcgacgctga
    22261 ggatcccgcc gtccgcatcg agtgaagccg cgaggctgct ggctgacgcg tccggaacgc
    22321 agggaggccg tccgctcgtg ggcctgcagg tgggggcggg ccgtgcggtg aaagagtggc
    22381 ccctgccgcg gctggccgag atcggcgcgt gggcccatcg cgaactcggc gctgcgctgg
    22441 tattgacggg ctcggccgcc gacggcgacg cggcccgtgc gctgcggggc tggctgccgc
    22501 ctgacgcgcg ggtcgccgat ctcaccgggg ccggcgatct cctgtcgctc gcggccgtgc
    22561 tctcccgcct ggccctcttc gtcacgccgg ataccgggcc catgcacctg gccgccgtca
    22621 tggggacgcc gctcgtcgcg gtgttcggcc cttcgtcgcc cgagcgctgg gggccgcttg
    22681 ctgccgcctg ccgcatcgtg cggatcgacc tgccgtgcag cccctgcaac cggatccgca
    22741 acccgccggc gcgctgccag gggcatacgc cggattgcct ggagggcatc gccgccgacc
    22801 aggtcatcgc cgccgcccgg gacctgctcg acgcgcggaa cacggagcac gccgatgg
//
LOCUS       DAOSIF010000006        22584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_2541_length_22584_cov_4.992010, whole genome shotgun sequence.
ACCESSION   DAOSIF010000006 DAOSIF010000000
VERSION     DAOSIF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 22584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22584
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_22584_cov_4.992010"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PLT35_00540"
     CDS             <1..1243
                     /locus_tag="PLT35_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27738.1"
                     /translation="ERPTLALRGDGTVFVLMAQPFRAYAKTLLEVQRYPVRKLPGGGA
                     PERPYDVAGWTLPYQMGVRVDRIDRPFDAPLLSRVDRVRPPVAWPFGYTSADYFLVDA
                     RGTAGALAASRLLAAGLRVEWSSGPVTQDGYKYGPGTLVVRPVSAARAHVERLAREAG
                     MRVFGARGRPPATLPLRLARTALYRPWNDAIDEGWTRWLLEQYEVPFATLRDAEARAG
                     RLRASFDVIVLPGIAAERLVDGNRKGSVPDEYAGGLSAAGVAALKAFVQEGGTLVCLA
                     GSGQLAIDALALPIKNVARGLSADRFFCPGSLIRLDVDASQPLGFGMLEKNAAFFSSD
                     SAYEIAQGAPGVRVAARYAAKDLLLSGWLEGEQVLAGRPAVVEARAGAGRAVLIGFRA
                     QHRGQSLATFRFLFNAIFMSP"
     gene            1496..1732
                     /locus_tag="PLT35_00545"
     CDS             1496..1732
                     /locus_tag="PLT35_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOG27739.1"
                     /translation="MPATWPWSPASARMIARAGDREAFRILVERHSRSAFRLACRLTG
                     HEQDAEDIVQETFLKAFRLFGRVELARKETDPAK"
     gene            complement(1913..3961)
                     /locus_tag="PLT35_00550"
     CDS             complement(1913..3961)
                     /locus_tag="PLT35_00550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG27740.1"
                     /translation="MRAWMMTVWLCALAVLAAPPALAAAQGPPAFTVEEMLKLKRLSD
                     PQLSPDGTRVAYVLTDVSLEENTRNNDIWVAPVAGGPAVRLAATAASEDRPRWSPDGR
                     QIAFVSTRSGSQQIWIIPAAGGEARQLTSIATGASGLTWSPDGKWLAFVSEVFPQCAD
                     EACNERELNALESSKVKARLADGLMYRHWTSWRDGRFSHLFIVPADGSAPPRDLTPGA
                     SDVPPFSLGGPEDYAFSPDSREIAFVRKTDRVEAISTNSDVFTLDLTADGAQPRQITA
                     GPGADGGPQYSPDGALLSWRSQARAGFEADIWKLNLLDRKSGSRRILADAWDRSIESW
                     TFTPDSAAVYVTAEESGSVRVYRLDLAGGAPKLVLRDGANGDIQFAPGGASFIFTRAS
                     LTAPAEIYKAASDGTRVAAVTNVNGAFLSAFALTPGESVMYAGAGGTPIQAWIVKPNT
                     FKAGVKYPLLCLVHGGPQSAWNDAWTYRWNAQVFASAGYVVFMPNPRGSTGFGQRFTD
                     AISNDWGGKPFDDVMKGVDYAERLPYVDSGRMAAAGASYGGYMMNWFLGHTDRFKAIV
                     SHAGVFNLTSMYGATEELWFPEWDLGGTPWTNPEGYARWSPHTYAKDFKTPTLVTHGE
                     LDFRVPVGEALQLFTTLQRQGIPSRLLVFPDEGHWIGKPQNAQRWYNTFLEWIGRWVN
                     "
     gene            4297..6690
                     /locus_tag="PLT35_00555"
     CDS             4297..6690
                     /locus_tag="PLT35_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG27741.1"
                     /translation="MHAVTRYGGCRQLDLDPLDADGRRDPRRRVALLEPFDKPARMAV
                     RASARVVSPRAWVAALGRAVLRRREPFGLVAPIEARIDLLPHQLEPALAVIHGGFTRL
                     LLADAVGLGKTIEAGLVLAELRARGGLSRGLVLVPPGLRDQWAAELADRFGLDATVAD
                     AAWLRARRAELPATVNPWSLPGIFVSSFDFAKRAEVLPGLEPLSWDALVLDEAHLAAG
                     DSERRAAAHALASRARVVLLLTATPHSGDPRVFQALCRIGCLGPGDRPAVFMRKRAQA
                     GLDRARRVSVLRVRASPAELELRRRLVGYIRRVWAREAGARPGNARLAMTVLLKRSFS
                     GVAPLRRSIEARLAGLEGGGPAAIQLPFAWDEDLDAADDEPFAALASPGLADAGEERA
                     VLQDLAREARAIEADDSKLRAVLKLIRRAPEPALVFTEYRDTLSALRQAAGESAGVLH
                     GGLDRAARAEALSRFVGGGTRVLLATDAAGEGLNLHERCRLVVNLELPWNPMRLEQRI
                     GRVDRIGQPREVRVVNLVAAGSAEERLFARLASRLERAREAVGPIENMLGSLDEDVVA
                     DQIGLGLPFVQGAWPAPAEPLEPAGPTVCPLELSARAGDLAAWLAVLRRLFRAAARRA
                     PRGGRGGRRRSAGGVAVAVRRHVALPAPLGRRGVLALFRVARGRPGGLAAWTELTPVF
                     VERRVPRLRRAGDIRALADAFIRDEAPALASAVAARFPRAGPAEPPFDRDACLARRAR
                     RRRAVQRGLFDARAEREARAEAEPAADAGAPAAAAGAQPSAAPEPAPELILLLFITG"
     gene            6709..10167
                     /locus_tag="PLT35_00560"
     CDS             6709..10167
                     /locus_tag="PLT35_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG27742.1"
                     /translation="MLDAISGSLVSRHFAEHVLPTAFAGRLGEPLCERARRALRAWWA
                     REGSALGPASSLRAVFDLGAAPIASLLGFSARKPRSALSGRALRADAISRAAAVPLLV
                     LPWGAPMGAFWNLAARECLAEGGRWVLTFNGRALRLCDLHRAYAREYLEFDLLRVAES
                     PAACSVFWGLLRPEALGALAPEIAEASARHGVAVTGLLKNGVRDALILLFQGLVSASR
                     KRTRPETDAERFARLAATLDQAYTIVYRILFLLFAESRTLVPVWHPVYRRGYTIEALR
                     SLAERPGSHAGIWEGLQAVSRLAHDGCRAGTLAVPAFNGRLFAPAHAPLAETARLDDG
                     LVARALVALTTVRVSGRRERVSFGDLGIEQLGAVYESVLEYEPAVTASGTRQPAGRRG
                     RASAPSVTLIAKRDARKSTGTFYTPRAITDYLVRRTLGPLVEGAAPDEILALRVLDPA
                     MGSGALLVASCRFLAEAYEAALVRDRGRFACDVTDDERAGFRRLIARRCLYGVDLNPM
                     AVHVARLSMWLATLAKGRPLSFLDHHLVCGDSLAGASPDDLARQPPVGRTARPKAGPL
                     PLFDAGETADALHAIVPIRNRLAATADETADIVREKSRLLAQLPARSAAYGSLRRAAD
                     LWCACWFWTGGAVPRPGPAEYEELARLVRTGVSSLPSRLAQSKLDEAGRLAAQRRFLH
                     WTLEFPEIFYDESGRVLPAPGFDAVLGNPPWEVLRGDTGSGEARESGRAAAASLARFA
                     RRSGLYRACDHGHANQYQLFVERSVRLLKPGGRLGLVLPWGLAADHGSAGLRRLLFER
                     CDTDTIVGFENSRRIFPIHRGVRFLLLSSSPGRPTKRTACRFGERDPAALERLEPGAR
                     ARGAARRAVTITPALLERIGGPGLAIPWVCSRADLRLVRRLSGSVPALADPRGWGAEF
                     GRELNRSDDRALLSAAPSGIPVVEGKHVDPFDVRLADCAWRVAGAAALPEGELRRAVG
                     RHRLAYRDVASATNRLTLIAAIVPPGAITVHTLFCLRGAAPLEDLLVLCCLLNSFVAN
                     YLVRLWVTTHLGAATVERLPLPRPRGNARPFGRLLELGRALFRARGRDDRAWAEVQGL
                     AARLYGLAEREFRLVLESFPLVDGAAKEAAADAFGRLSARSEVGVRSRKPVPDREPGC
                     AGVDRR"
     gene            complement(10195..11124)
                     /locus_tag="PLT35_00565"
     CDS             complement(10195..11124)
                     /locus_tag="PLT35_00565"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOG27743.1"
                     /translation="MHFAILGSGAVGGYYGALLARSGQRVSFVARGAHLRAIRERGLM
                     IWSPLGDFTVRAQAEDDAAAIGPVDVVLLAVKTYDNAAVLPMLPALVGPSTVVITLQN
                     GVDSPDEVAAVVGKERVLGGPTYIAAARSLPGLVEQTGTHRRIVFGEAFGGHGGVSQR
                     VSTIADALTAAGIQAETVANARVPLWEKFIYLAPFAAFTGAARLPIGPLWSDPSIRAQ
                     FMAAVGEVEAVARAEGVPVREPADLREYVSTYVDALPPHTKSSLLIDLQQGKRTEVES
                     LAGSVVRRGCAAGVPTPIMSALYAVLKPWGQLR"
     gene            complement(11137..11802)
                     /locus_tag="PLT35_00570"
     CDS             complement(11137..11802)
                     /locus_tag="PLT35_00570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG27744.1"
                     /translation="MAARTLALFDLDDTLLAGDSDYEWGLFLAEIGAIDEAGYKARND
                     EFMAQYRAGTLDLQAFLAFQLCPLGQFSMAQLEAWHAQFMERKILPIVRPGSRALLDK
                     HRGAVQVMVTATNEFVTAPIAAAFGIPALIATALERADGRFTGRTVGTPSFREGKVVR
                     LKEWLASRGERLEDYAESWFYSDSINDVPLMSLVTHPVAVHPDERLRAHALERGWPII
                     RLD"
     gene            complement(11864..13303)
                     /locus_tag="PLT35_00575"
     CDS             complement(11864..13303)
                     /locus_tag="PLT35_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG27745.1"
                     /translation="MRMNGPRTPRYGSRGGSAGALTAILAAIALAAGCGATARPAVTH
                     PGGRAAAAAIPPSWSPQQLADAWDRERVSWPVPALVRHADVESRLRDMQAQSPDLFRL
                     EEFGRSVEGRPLYRLWFGRGPLHVLLWSQMHGDEPTATAALFDVFDHVRRRRADPAVV
                     RMLDALTIHAVPMLNPDGAERFQRRNAQGLDINRDALRLQTPEGRALKVLRDRLQPAI
                     GFNLHNQGWRTSAGKTAKPASISLLSVAFDEPGTMSPGRVLTKKTAALIRDALEPLAP
                     GRIARYGDEFEARAFGDNLTKWGTSVLLIETGPWPAADPDPHLVRLNFVALMTALDAL
                     ATGRVARADERRYDSLPVNDTALFYLLIRNATVVPGTGVAPFIADIGIVAARGIREAD
                     GGREVRLAARIEDLGDLSTMKGMTEVDAAGLVVAPLWDPALKEGDAVALPDFSAHRAA
                     RAVAPGAPADLVILRPEAGRHRVVRVITH"
     gene            13423..14835
                     /locus_tag="PLT35_00580"
     CDS             13423..14835
                     /locus_tag="PLT35_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27746.1"
                     /translation="MIDSLTPDGPSADLHQLHHALRLQIGKPPEAWGADDLAAFVRDR
                     RVRLLSLMHVGGDGWLKTLDFVPRDSEHLVDVLLGGERADGSSLFGDLGIPSGASDIM
                     LRPRLSTAFLDPFSSEPSLALLCSHYNRHGAPLAESPDTLVRSAYRRLVTATGVELHA
                     LGEIEYFLGTRSGEASAYGTSERGYHASSPFVFGEALRRTALVRLAEMGVPVKYGHAE
                     VGYVEAEAADGRAWEQHEIELWLRPLPEAADAVVLTQWVLRNLAQRAGMLCSFAPMAR
                     KGHAGSGLHFHFAPLKEQAYLPHTGADGGLAPEAQWLIGGLVTHGAAMMAFGNRDRDS
                     FVRLSQAKEAPNAVTWGRYNRRALVRIPIIPVDQAGRQTSPGTVEFRLPDGSAHPHLL
                     LAGIAQAFVDGHAMTDLDARLARTAVTAHGHAASENRLPLAFPEVADALAASRSMLEA
                     GGVFTANLLDRVIALLRARD"
     gene            complement(14873..15706)
                     /locus_tag="PLT35_00585"
     CDS             complement(14873..15706)
                     /locus_tag="PLT35_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27747.1"
                     /translation="MTARERLPACLAFPLAALLAVAALGGILLPGVYAEAHPRWAAQG
                     VGQDWVDLVLVAPLLSLAAHGVLRGSRVASLVLAGMLAYVLYSMVVYAFFLHFGPLFL
                     VYTAALGLSFYALVAQVCALNAGDVRGWFEADAPIRPAGAVSVLLGVMFGALWLAEIV
                     PALAGATGLRSAREAGLITNPVEVLDLGVVLPAFIVGGLLLLRRRPSGYWLVPTMLAF
                     AVVMDAALVGMTVSVRAKIGAEGAPLAFLIGSAVVTVAVLALLLRSVKGSGRFLQTTI
                     P"
     gene            complement(15716..16288)
                     /locus_tag="PLT35_00590"
     CDS             complement(15716..16288)
                     /locus_tag="PLT35_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27748.1"
                     /translation="MDGRAVPVIAALVLAGACTNVKKVPEVSDVRADIDAVNEELVDC
                     WNAGEYECVAAAFTEDGWQMMPHTTARVGTDDIQRYWQLAFGWGHWEVSLATSLLETS
                     GPVAIERGKYTIQFAADASAPPGRPSSQDRGNYVTHWRLDAAGRWRIAAQALVSEIPA
                     RVVPVQPVQQAQPFQSPDPGQPPAPPGSPQ"
     gene            17035..19242
                     /locus_tag="PLT35_00595"
     CDS             17035..19242
                     /locus_tag="PLT35_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG27749.1"
                     /translation="MSVAYDYKVRTEYKGDVPRLRAAHTVVAEFPFQASLPIRGYTRR
                     TLFIDLDTMSVREKPVTEEMIDIFTGGRGFGMYYLWHAITPETKWNDPANEIIISPGP
                     IAGNPQYAGSGKSLVVTISPMTGIPIDCNVGGYFGPLLKFCGFDALEIRGKAKADTVI
                     FIDGPSGLIRLEEAPEETPDSHVAAEVFTHMYAADAKDLQNVSVVSAGKAAETAYMGC
                     LNFSWYDPKRGAVRLKQAGRGGTGTVFRDKRLKALVVRGPKMGPNLNNAADLPSVIRV
                     GRIINQEIHDHDDEQCRMRRQGTAHLVEIMDAYDLLPVHNFQYGKHLDTHRIDSQVWD
                     KRFTQAVPDRCWYGCGMACSKGADGHIVRTGPYKGHIVTVDGPEYENVGGLASNCGLF
                     DPDWLLEVNFYCDTYGIDTISYGTTCAFIMECWEKGILNAERTGGIDLSWGNGESEVE
                     LLHQMWRGEGFGVHVGKGVRYLQDLFIENGWGDPQFIRDIGMQIKGLEISQYMSKESL
                     AQQGGYALANKGPQHDEAWLIFMDMVNNQIPTFEDKAEALHYFPLFRTWFGLQGLCKL
                     PWNDIEPPDNHEKYPGPEAAKVPAHVDNYLALYTGITGRPLDRASLLEQSERVYNFQR
                     VFNLRMGQGVRAFDMPPYRAMGPVTKAEYESRAERYDAQLKDIVGIDPAGKSTEEKMA
                     LHRQWRTDRYNRLVDAVYKRRGWTLNGVPTLETLKKLRIDFPEVVEVVKAHQE"
     gene            19382..19570
                     /gene="thiS"
                     /locus_tag="PLT35_00600"
     CDS             19382..19570
                     /gene="thiS"
                     /locus_tag="PLT35_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOG27750.1"
                     /translation="MNDTPLEFEAGMTVRDILRRRNYVWRMIAVYVNGELVPRTTYDT
                     RQVPDGADVKVIHQIAGG"
     gene            19723..20184
                     /gene="bcp"
                     /locus_tag="PLT35_00605"
     CDS             19723..20184
                     /gene="bcp"
                     /locus_tag="PLT35_00605"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOG27751.1"
                     /translation="MAQLKVGDRAPAFALEDQRGRTVRLSDFKGRKLLVYFYPKANTP
                     GCTAQSCSVRDARTDFARKGVAVIGVSPDKPSAQRRFDEKHGLGFPLLADTDHVMAEA
                     YGVWGEKSMYGRTYFGIVRSSFLIDERGRIAGAWYKVSPAGTVPEALNALG"
     gene            complement(20239..21705)
                     /locus_tag="PLT35_00610"
     CDS             complement(20239..21705)
                     /locus_tag="PLT35_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HOG27752.1"
                     /translation="MNRNTLYTILTAAVLALLIGPIGVAVFVLGFVHGDSPCVLCWAQ
                     RTAMVLVALTGLLIVRYGPRPRYIGMGVLISAFGVYMGLRHSSLHFARDVGQGFSAEI
                     LGAHTYTWSLFIFWVCLVMMGAMLLAVKDGEAAGEGPRELRPPGRAALWLFLLAVAAN
                     MVQAFASTGPPPFMGQGDPVRFSFNPRNWVWSTEEWRPSPVSLRGRWAIEKPDVALAP
                     ADPAGSPLAGVPAMTVSRRAKLALPLNGPVTDLAYDAASDRFLLTTHNGVYLTDGTLG
                     RVIRHTVVDVGYSVDLARFSAAAFLDSRTVLAMAENKSYVVLRESDQADAAANYRYFL
                     ESPGAFEEASRSRLTTVRAKMMYVMAAAFDPAASALYTVTVPNAASKRLVVSRFDRRD
                     MTLAEEFSPALAEGGPLAFKDGAGSLDGFYPTAAEFHEGRLYVLSAAYSTLLVVDPVR
                     RTVADARVVPGLSKPSGIAIKGGELLIVTEDGALSWAH"
     gene            complement(21735..21902)
                     /locus_tag="PLT35_00615"
     CDS             complement(21735..21902)
                     /locus_tag="PLT35_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27753.1"
                     /translation="MELLNFILIFAAGWLVLRRPERERLAFGLLVASSLLMAALFLIG
                     ARGSILPGLNY"
     gene            complement(22031..>22584)
                     /locus_tag="PLT35_00620"
     CDS             complement(22031..>22584)
                     /locus_tag="PLT35_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG27754.1"
                     /translation="WPGQARTVWGNHRLFRDTYFTTYPGYYFTGDGATRDEDGYYWIT
                     GRIDDVLNVSGHRIGTAEIESALVAHDAVAEAAVVGYPHEIKGTGIYCYVVLHSDFAE
                     GDPEQLVGALKQQVRNAIGAFATPDIIHVASGLPKTRSGKIMRRILRKVAASEYDGLG
                     DTSTLAEPEVVQRLIEERRHAHG"
BASE COUNT         3155 a         8127 c         8012 g         3290 t
ORIGIN      
        1 ggagcggccc accctggcgc tacgcggcga tggcacggtc ttcgtcctga tggcgcagcc
       61 gttcagggcg tacgccaaga ccctgctcga ggtgcagcgc taccccgtgc ggaagctccc
      121 gggcggcgga gcgcccgagc gcccgtacga cgtcgcgggc tggacgctgc cctaccagat
      181 gggcgttcga gtggatcgga tcgaccggcc gttcgatgcg ccgctcctgt cgagagtcga
      241 tcgcgtccgg ccgccggtcg catggccgtt cgggtacacg agcgccgact acttcctcgt
      301 ggacgcccgc ggcacggcgg gcgcgctggc ggcgagccgc ctcctggcgg cggggctgcg
      361 cgtcgagtgg tccagcggac ccgtcacaca ggacgggtac aagtacgggc cgggcacgct
      421 cgtcgtgcgc ccggtgtcgg ccgcccgcgc gcacgtcgag cgcctcgcgc gcgaggcggg
      481 catgcgcgtg ttcggcgccc gcggcaggcc gcccgcgacc ctgccgctcc gactggcccg
      541 aaccgcgctc taccggccgt ggaacgacgc gatcgacgag ggatggacgc gctggctgct
      601 ggagcagtac gaggtcccgt tcgccacgct ccgcgacgcc gaggcgcggg ccgggcggct
      661 ccgcgcctcc ttcgacgtca tcgtcctgcc cggcatcgcg gcagagcggc tcgtcgatgg
      721 caaccggaag ggatccgtgc cggacgagta cgccggcggc ctcagcgcgg ccggcgtcgc
      781 cgccctcaag gcgttcgtgc aggagggcgg cacgctggtg tgcctggcgg gctccgggca
      841 gctggccatc gacgccctcg cgctgccgat caagaacgtc gcgcgcggcc tgagcgccga
      901 caggttcttc tgccccggct cgctcatccg gctcgacgtc gacgcgtcgc agccgctcgg
      961 gttcggcatg ctcgagaaga acgcggcgtt cttctcgtcg gactcggcct acgagatcgc
     1021 ccagggcgcg cccggcgtcc gcgtcgcggc gcgctacgca gcgaaggacc tcctgctgag
     1081 cggatggctg gaaggtgaac aggtcctcgc ggggcgcccg gcggtggtcg aggccagggc
     1141 cggcgccggc cgggccgtgc tcatcggctt ccgcgcgcag caccgcggcc agtcgctggc
     1201 cacgttccgg ttcctcttca acgccatctt catgagccct tgatccccgg cgccctccat
     1261 ctcccggcct ctgccttccg ggccttgttt tctgttttct ggactctgaa ttccggattc
     1321 tgaattctct gcctccttca cctcctcccc ttccttgccc ccgtcttcgc caagcggcct
     1381 gccgatccgc ttcggcgagg caggctccct cccttgtccc tgtccgacca gcgcctggcg
     1441 ccaagccccg aacgcctgac tgaatccccc gcccttcgtc tgaccgtgtg aatcgatgcc
     1501 agcgacctgg ccgtggtcgc ccgcgtccgc gcggatgatc gcgcgcgcgg gtgaccgcga
     1561 ggccttcagg atcctcgtgg agcgccacag ccggagtgcg ttcaggctgg cctgccgcct
     1621 gacggggcac gagcaggacg cggaagacat cgtccaggag acgttcttga aggctttccg
     1681 gctgttcggg cgcgtcgagt tggcccgcaa ggagaccgat ccggcgaaat agcgcagcgt
     1741 cgagcgcttc agcctgatgc gctcgaagga cgccaccgac tacaagatgg agatcatcgg
     1801 gaagtcgcca gggaccaggc gctcggggct ggtcagattc gctgttgaac tgccggccac
     1861 gcggcgaaag ccgcgtccga ctagcgccca gcgcctggcc cctggccccc ggctagttca
     1921 cccaccgtcc gatccattcc aggaaggtgt tgtaccagcg ctgcgcgttc tgcggcttgc
     1981 cgatccagtg gccctcgtcc gggaacacca ggagacggga cgggatgccc tgccgctgga
     2041 gggtggtgaa gagctgcagg gcctcgccga cgggcacgcg gaagtcgagc tcgccgtggg
     2101 tgacgagggt cggcgtcttg aagtccttcg cgtaggtgtg cggcgaccac ctggcgtagc
     2161 cttccgggtt cgtccacggc gtgccgccga ggtcccactc ggggaaccac agctcctcgg
     2221 tcgcgccgta catgctggtg aggttgaaca cgccggcgtg cgacacgatg gccttgaagc
     2281 gatcggtgtg gccgaggaac caattcatca tgtagccgcc gtaggacgcg ccggccgcgg
     2341 ccatgcggcc cgagtccacg tagggcagcc gctcggcgta gtccacgccc ttcatgacgt
     2401 cgtcgaaggg cttgccgccc cagtcgttgc tgatcgcgtc ggtgaaccgc tgcccgaacc
     2461 ccgtcgagcc gcgcgggttc ggcatgaaca cgacgtagcc cgccgacgcg aagacctggg
     2521 cgttccagcg gtacgtccac gcgtcgttcc acgcgctctg gggccccccg tgcacgaggc
     2581 acagcagcgg gtacttcacc cccgccttga aggtgttcgg cttgacgatc caagcctgga
     2641 tcggcgtgcc gcccgcgccg gcgtacatca cgctctcgcc cggcgtgagg gcgaacgccg
     2701 agagaaaggc cccgttgacg ttcgtcaccg ccgcgacgcg cgtgccgtcg gaggcggcct
     2761 tgtagatctc ggcgggagcg gtgagcgacg cgcgcgtgaa gatgaacgac gccccgcctg
     2821 gcgcgaactg gatgtcgccg ttcgcgccgt cgcgcagcac cagcttcggc gcgccgcccg
     2881 cgaggtcgag ccggtagacg cgcacgctcc cgctctcctc ggccgtcacg tacacggcgg
     2941 cggagtcagg cgtgaaggtc cacgactcga tcgagcggtc ccacgcatcg gcgaggatcc
     3001 tccgggagcc ggacttgcgg tcgagcaggt tgagcttcca gatgtccgcc tcgaatcccg
     3061 cgcgtgcctg ggaccgccag ctcagcagcg ccccgtccgg cgagtactgc ggcccgccgt
     3121 ctgcgcccgg tccggcggtg atctgcctgg gctgcgcgcc gtcggccgtc aggtccagcg
     3181 tgaagacgtc gctgttcgtg ctgatagcct cgacccggtc cgtcttcctc acgaaggcga
     3241 tttccctgga atcgggcgag aacgcgtagt cctcggggcc gccgagcgaa aagggcggca
     3301 cgtcggaggc gccgggcgtc agatcgcgcg gcggcgcgga gccgtccgcc gggacgatga
     3361 acaggtggct gaatcggccg tcccgccagg acgtccagtg gcggtacatc aggccgtcgg
     3421 ccaggcgcgc cttcaccttc gacgactcga gggcgttcag ttcgcgctcg ttgcaggctt
     3481 cgtccgcgca ctgcgggaag acctcggaca cgaacgccag ccacttgccg tcgggcgacc
     3541 aggtgaggcc tgatgccccg gtcgcaatcg acgtgagctg gcgcgcctcg ccgccggcgg
     3601 cggggatgat ccagatctgc tggctgccgc tgcgcgtgga cacgaacgcg atctgtctgc
     3661 cgtcgggcga ccagcgcggg cggtcttcgg acgccgccgt ggcggccagt ctcaccgccg
     3721 ggccgcctgc gacgggcgcg acccagatgt cgttgttgcg ggtgttctct tcgaggctga
     3781 cgtcggtcag gacgtaggcc acgcgcgtcc cgtctggcga caactgcggg tccgataggc
     3841 gcttcagctt cagcatctcc tcgacggtga acgccggcgg cccctgggcc gccgccagcg
     3901 caggcggcgc ggccagcacg gccagcgcgc acaaccacac cgtcatcatc caagcccgca
     3961 tgcagcgttc catctcttgt ccccgccttc gctaaacggc ccgccggccg gccctgagct
     4021 tgtcgacggg ccggcttcgg cgcggcaagc cttgctcctc gtcccgagtc ccgggtcccg
     4081 actctcgact cccgagtccc gagccccgag attagcacgc cccttgctcc tggatcctcc
     4141 cggacgggcc ggttcaggcg catttgcggg agcctcccgg ccgcgggccg cgaagcgtgg
     4201 cagtttccgc caccgcgttc ggccgcgagg tcacaatccg tgcaacctgc cttttcgccc
     4261 ggcgagctcg tccgcgtgcg agggcgtgac tggcgcgtgc acgcggtcac tcgctacggc
     4321 ggctgccgcc agctggacct ggatccgctc gacgccgacg ggaggcgcga cccccgccgc
     4381 agggtggcgc tgctcgagcc gttcgacaag ccggcgcgga tggccgttcg cgcatcggct
     4441 cgcgtggtct cgccccgggc ctgggtggcg gccctcggcc gcgccgtgct ccgccggcgg
     4501 gagccgttcg gcctcgtcgc tccgatcgag gcgcggatcg atctgcttcc ccaccagctc
     4561 gaaccggcgc tcgccgtgat ccatggcggc ttcaccaggc tgctcctggc cgacgcggtg
     4621 ggcctcggca agacgatcga ggccggcctc gtgctggcgg agcttcgcgc gcgcggcggc
     4681 ttgtcgcgcg gcctcgtcct cgtgccgccg ggccttcgcg atcagtgggc ggcggagctg
     4741 gccgatcggt tcggtctcga cgccacggtg gcggacgcgg cctggctgcg cgcgcgccgc
     4801 gcggaactgc ctgcaacggt caatccctgg tcgctgcccg ggatcttcgt ctcgtccttc
     4861 gacttcgcga agcgcgccga ggtgctgccg ggcctcgagc ccctctcctg ggacgccctc
     4921 gtgctggacg aggctcacct ggcggccggc gacagcgagc gccgcgcggc ggcccacgcg
     4981 ctggcgtcgc gcgcgcgggt cgtgctgctg ctgaccgcca cgccgcactc cggcgacccg
     5041 cgcgtcttcc aggcgctctg ccggatcgga tgcctgggcc ccggcgatcg cccggcggtc
     5101 ttcatgcgaa agcgcgcgca ggctggcctc gatcgtgcgc gtcgcgtctc ggtcctccgc
     5161 gtgcgcgcca gcccggcgga actggagctt cgccgccggc tcgtcggcta catccgccgc
     5221 gtctgggcgc gggaggccgg cgcccgtcct ggcaacgcgc gcctcgccat gaccgtcctg
     5281 ctgaagcgct cgttctcggg cgtggcgccg ctccggcgct ccatcgaggc gcgcctggcg
     5341 gggctcgaag gcggcgggcc ggccgcgata cagctgccgt tcgcgtggga cgaggatctc
     5401 gacgcggccg acgatgagcc gttcgccgct ctggcgtccc ccggcctcgc tgacgcgggc
     5461 gaggaacggg ccgtcctcca ggatctggct cgggaggccc gggcgattga agcagacgac
     5521 tcgaagctgc gggccgtgct caagctgatc cggcgcgcgc cggagcccgc cctcgtcttc
     5581 accgagtacc gcgacacgct gtcggccctg cgccaggccg caggagagag cgccggcgtc
     5641 ctgcacggcg gcctcgaccg ggcggcgcgc gccgaggcgc tctcgcgctt cgttggcggc
     5701 ggcacgcgcg tcctgctggc caccgatgcc gccggcgagg gcttgaacct ccacgagcgc
     5761 tgccggctcg tcgtcaatct tgagttgccg tggaacccca tgcgcctgga gcagcgcatc
     5821 gggcgcgtgg accggatcgg ccagccgcgc gaggttcgcg tcgtcaatct cgtggccgcc
     5881 ggctcggccg aagaacggct gtttgcgcgg ctggcctccc gcctcgagcg cgcccgggag
     5941 gccgtcggcc cgatcgagaa catgctcggc agcctcgacg aggacgtggt cgccgaccag
     6001 atcggactcg gccttccgtt cgtccaaggc gcctggcccg cgccagccga accgctcgag
     6061 ccggccggcc cgacggtctg cccgctcgag ttgtcggcgc gtgcggggga tctcgcggcc
     6121 tggctggctg tgctccggcg cctcttccgc gcggcggcgc gaagagcgcc gcgcggcgga
     6181 cgcggtggtc ggaggcggtc tgccggaggc gttgccgtgg cggtccgccg gcacgttgcg
     6241 ttgccggccc cgctcgggcg ccgcggggtg ctcgcgctct tccgcgtggc gcgcggccgc
     6301 ccgggcggcc tggccgcatg gaccgaactg acgccggtct tcgtggagcg gcgcgtcccg
     6361 aggctccgac gcgcaggaga catccgcgcc ctcgccgacg ccttcattcg cgacgaggcg
     6421 ccggccctcg cctccgccgt cgcggcgcgt tttccgcgcg ccgggccggc cgagcctccc
     6481 ttcgaccgtg atgcctgcct cgcgcgccgc gcccgtcggc gccgagccgt ccagcgcggc
     6541 ttgttcgacg cgcgggcgga gcgcgaggcg cgcgccgagg cggaacccgc ggcggacgcc
     6601 ggcgcgccgg ctgccgctgc cggagcgcag ccgtccgccg cgccggagcc cgcgccggag
     6661 ctgatcctcc ttctcttcat caccggctga tcgccaggac ggcgcaccgt gctcgacgcc
     6721 atcagcggct cgctcgtctc gcggcacttc gcagaacacg tgctgccgac ggccttcgcc
     6781 ggccggctcg gcgagccgtt gtgcgagcgc gcgcggcgcg cgctccgcgc ctggtgggcg
     6841 cgcgagggtt cggccctcgg accagcctcc tcactgcgag cggtcttcga tctgggcgcg
     6901 gcgccaatcg cgtccctgct cggcttctcc gcgcgcaagc cgcggtccgc gctgtccggc
     6961 cgggccctcc gggccgacgc catctcccgc gccgccgccg ttccccttct cgtcctgccc
     7021 tggggcgcgc ccatgggcgc tttctggaac ctcgcggcgc gcgagtgcct cgccgagggc
     7081 ggccgctggg tgctgacgtt caacgggcgc gcgctcaggc tgtgcgacct gcaccgggcg
     7141 tatgccaggg agtacctgga gttcgacctg ctgcgcgtgg ccgaaagccc cgcggcctgc
     7201 agcgtgttct gggggctgct tcggccagag gcgctcgggg ccctggcgcc ggaaatcgcg
     7261 gaggcgtcgg cgcggcacgg cgtggccgtg accgggctgc tcaagaacgg cgtccgcgac
     7321 gcgctgatcc tgctcttcca gggcctcgtc tccgcgtcac gaaagcgcac ccgcccggag
     7381 acggacgccg agcgtttcgc ccgtctcgcc gcgacgctcg accaggcgta caccattgtc
     7441 taccgcatcc tgttcctgct cttcgcggag tcgcgcacgc tcgtgcccgt ctggcacccc
     7501 gtctaccggc gcggctacac gatcgaggcg cttcgctcgc tggccgagcg gccgggctcg
     7561 cacgccggga tctgggaggg gctgcaggcc gtgtcccgtc tggcgcacga cggctgccgc
     7621 gcgggcacgc tcgccgtgcc ggccttcaac gggaggctgt tcgcgccggc gcacgcgccg
     7681 ctggcggaga cggcccgcct cgacgacggg ctcgtggccc gggcgctcgt ggccctgacg
     7741 accgtccgcg tgtcagggcg ccgcgagcgg gtgtcgttcg gcgacctcgg catcgagcag
     7801 ctcggcgcgg tctacgagag cgtgctggag tacgagccgg cagtgaccgc gagcggaacc
     7861 cgccagccgg ctggccggcg cggacgcgcg tcggcgccgt cggtgacgct gatcgcgaaa
     7921 cgggacgcgc gaaagtccac cggcacgttc tacacgccgc gcgcgatcac ggactacctg
     7981 gtgcgccgca ctctcggccc gctcgtggag ggcgcggcgc cggacgagat cctcgccctt
     8041 cgcgttctcg acccggcgat gggcagcggc gcgctgctcg tcgcgtcgtg ccggttcctc
     8101 gccgaggcct acgaggcggc gctcgtccgg gatcgaggcc gcttcgcgtg cgacgtgacc
     8161 gacgacgagc gcgccgggtt tcggcgcctc atcgcccggc gctgcctcta tggggtggat
     8221 ctcaacccga tggccgtcca cgtggcgcgc ttgtccatgt ggctggcgac cctcgcgaag
     8281 ggccgcccac tgtcctttct cgatcaccat ctcgtctgcg gcgacagcct cgcgggagcg
     8341 tcgccggacg atctcgcgcg gcagccgccc gtagggcgga cggcccgccc caaggcgggc
     8401 ccgctcccgc tgttcgacgc cggcgagaca gctgacgcgc tgcacgcgat cgtgccgatc
     8461 cggaaccggc tggccgccac cgccgacgag acggccgaca tcgtgcgcga gaaatcgcgc
     8521 ctactcgcgc agctgcccgc gcgctctgcg gcgtacgggt cgctgcggcg cgccgccgac
     8581 ttgtggtgcg cgtgctggtt ctggacgggc ggcgcggtgc cccggcccgg cccggccgag
     8641 tacgaggagc tcgcccgcct ggtgcgaacg ggcgtgtcga gcctgccgtc gcggctggcg
     8701 cagtcgaagc tcgatgaagc cggccggctc gcggcgcagc gcaggttcct gcactggacg
     8761 ctcgagttcc ccgagatctt ctacgacgaa tccggccgtg tgctgcccgc gcccgggttc
     8821 gatgcggtgc tgggcaatcc gccgtgggag gtgcttcgcg gcgacaccgg ctcgggcgag
     8881 gcgcgcgaga gcgggcgcgc ggcggcggcg agcctcgcgc ggttcgcgcg tcggtccggc
     8941 ctctaccggg cgtgcgacca cggccacgcc aaccagtacc agttgttcgt cgagcggtcc
     9001 gtccggctgc tgaagcccgg cggccggctc ggcctcgtgc tgccgtgggg gctggccgcc
     9061 gatcacggca gcgccgggtt gaggcggctc ctgttcgagc gctgcgacac cgacaccatc
     9121 gtcgggttcg agaacagcag gcggatcttc cccattcacc gcggcgtgag gttccttctc
     9181 ttgtcgagtt cgcccgggcg gccgacgaag aggaccgcct gccggttcgg cgagcgcgat
     9241 ccggccgcgc tcgagcgcct ggagcccggc gcgcgggccc gcggcgcggc ccggcgggcg
     9301 gtcacgatca cgccggcttt gctcgagcgc atcggcgggc ccggcctggc gattccctgg
     9361 gtgtgcagcc gggccgatct gcggctggtc cgccggctca gcggcagcgt gcccgcgctg
     9421 gcggatccgc gcggctgggg agccgagttc gggcgcgagc tgaaccgcag cgacgaccgc
     9481 gccctgctct ctgcggcgcc gtccgggatc ccggtcgtcg agggcaagca cgtcgatccg
     9541 ttcgacgtgc gcctggcgga ctgcgcgtgg cgcgtggctg gcgcggccgc gctgcccgag
     9601 ggcgagctgc gccgggcggt aggccggcac cgtttggcct atcgcgacgt ggcgtcggcg
     9661 acgaaccggc tgacgctcat cgccgccatc gtgccgcctg gcgcgatcac ggtccacacg
     9721 ctcttctgcc tcaggggggc cgcgccgctc gaagacctgc tggtgctctg ctgcctgctc
     9781 aacagcttcg tcgcgaacta cctcgtgcgg ctgtgggtga cgacgcacct cggggcggcg
     9841 actgtcgaga ggctgcccct gccgcggcct cgcgggaacg ctcggccgtt cggccgtctg
     9901 ctcgagcttg gccgcgccct gttccgcgcg cgcggccggg acgatcgcgc ctgggccgag
     9961 gtccagggcc tcgccgcgcg cttgtacggc ctggcggagc gggagttccg tctcgtgctc
    10021 gagtcgttcc ccctggtgga cggcgcggcg aaggaggccg cagccgacgc cttcgggcga
    10081 ctgtcagccc gctcggaggt tggggtcaga tccaggaaac cggtgccaga tcgagaacct
    10141 ggctgtgctg gcgtggatcg gcgctgacca tcgccatctc cggggccgcc ctgatcagcg
    10201 gagctggccc cacggcttca ggacggcgta cagggccgac atgattggcg tcggcacgcc
    10261 tgccgcgcat ccgcgccgca ccaccgaacc cgccagcgac tccacttccg ttcttttccc
    10321 ctgctggaga tcgatgagca gcgacgattt cgtgtgaggc ggcagcgcgt ccacgtacgt
    10381 cgagacgtac tccctgagat ccgccggctc gcgtacgggc acgccctcgg cccgagccac
    10441 cgcttcgacc tctccgacgg cggccatgaa ctgcgctcta atcgaggggt cgctccacag
    10501 cgggccgatg ggcaggcgcg ccgcgcccgt gaaggccgcg aacggcgcga ggtagatgaa
    10561 cttctcccac agcggcacgc gcgcgttggc gacggtctcg gcctggatgc ccgcggcggt
    10621 cagcgcgtcg gcgatcgtgc tcacccgttg cgagacgccg ccgtgcccgc cgaaggcctc
    10681 gccgaacacg atccgccggt gcgtgcccgt ctgttcgacg agccccggca gcgaccgcgc
    10741 cgccgcgatg taggtcggcc ctccgaggac gcgctccttc ccgacgaccg ccgccacttc
    10801 gtcggggctg tccacgccgt tctggagggt gatgacgacg gttgacggcc cgaccagcgc
    10861 gggcagcatc ggcagcaccg cggcgttgtc gtaggtcttg acggccagca gcacgacatc
    10921 gaccggcccg atggcggccg cgtcgtcttc ggcctgtgcc cgcacggtga agtcgccgag
    10981 cgggctccag atcatcaggc cgcgctcgcg gatggcgcgc aggtgggcgc cgcgcgccac
    11041 gaagctgacg cgctggcccg atcgggcgag cagggccccg tagtagccgc cgacggcgcc
    11101 ggatccgagg atcgcgaagt gcatgggaag gtcctctcag tcgagcctga tgatcggcca
    11161 gccgcgctcg agtgcgtgcg cccgcaggcg ttcgtcggga tgcacggcca ccgggtgcgt
    11221 cacgagcgac atgagcggca cgtcgttgat tgaatcgctg tagaaccagc tctccgcgta
    11281 gtcctcgagg cgctcgccgc gcgacgcgag ccattccttc aggcggacga ccttgccctc
    11341 gcggaaactc ggcgttccga cggtgcgccc ggtgaagcgg ccgtcggcgc gctccaacgc
    11401 cgtcgcgatg agggctggaa tgccgaacgc cgcggcgatc ggggcggtca cgaactcgtt
    11461 cgtggcggtg accatcacct gcaccgcgcc gcggtgcttg tcgaggaggg cgcgggagcc
    11521 gggcctgacg atcggcagga tcttccgctc catgaactgc gcgtgccagg cctcgagctg
    11581 cgccatgctg aactggccca gggggcagag ctggaacgcg aggaacgcct gcaggtccag
    11641 cgtgccggcc cggtactgag ccatgaactc gtcgttgcgc gctttgtacc cggcctcgtc
    11701 tatcgcgccg atctcggcca ggaacaggcc ccactcgtag tcgctgtcgc cggcaaggag
    11761 cgtgtcgtcc aggtcgaaga gcgccagtgt ccgtgctgcc atgcgttgat gcttctccag
    11821 atgccgggcc tgtgcctctc gagaggccgc gtcccggggc cgatcagtgc gtgatgaccc
    11881 tcacgacccg atgccgtccg gcctccggcc tgaggatgac caggtccgcc ggcgctcccg
    11941 gcgccacggc gcgagcggct ctgtgcgcgg agaagtccgg cagcgccacc gcgtcgccct
    12001 ccttcagcgc ggggtcccac aacggcgcca cgaccaggcc ggcggcgtcc acttccgtca
    12061 tccctttcat tgtgctcaag tcgccgagat cctcgatgcg cgccgccagc cggacctcgc
    12121 gcccgccgtc ggcctctctg atcccgcgcg cggcgacgat gccgatgtcc gcgatgaagg
    12181 gcgcgacgcc ggtgccggga acgaccgtcg cgttccggat cagcaggtag aagagggccg
    12241 tgtcgttcac cggcagcgag tcgtacctgc gctcgtcggc gcgcgcgacc cgccccgtgg
    12301 ccagcgcgtc gagcgccgtc atcagcgcga cgaagttcag gcgcaccagg tgcgggtccg
    12361 gatcggcggc gggccacggc ccggtctcga tcagcaggac gctcgtcccc cacttcgtga
    12421 ggttgtcgcc gaacgcccgc gcctcgaatt cgtcgccgta gcgggcgatg cgccccggcg
    12481 cgagaggctc gagggcgtcg cggatgaggg ctgccgtctt cttcgtgagg acgcgcccgg
    12541 gactcatcgt gcccggctcg tcgaaggcca ccgacagcag cgagatcgaa gccggcttcg
    12601 cggtcttgcc cgccgaagtg cgccagccct ggttgtggag gttgaatccg attgccggct
    12661 ggagccggtc ccgcagcacc ttgagcgcgc ggccctcggg cgtttgcagg cgcagcgcgt
    12721 cccggttgat gtcgaggccc tgggcgttcc gccgctggaa acgctccgcg ccgtccgggt
    12781 tgagcatggg cacggcgtgg atggtcagcg cgtcgagcat ccggacgact gccggatcgg
    12841 cgcgccgcct ccggacgtga tcgaacacgt cgaagagcgc cgccgtggcg gtcggctcgt
    12901 cgccgtgcat ctgcgaccag agcaggacat ggagcgggcc gcggccgaac cagagacggt
    12961 agagcggccg cccctcgacc gagcggccga attcctcgag gcgaaagaga tccggcgact
    13021 gcgcctgcat gtccctgagg cgcgattcga cgtcggcgtg gcgaacgagg gcgggaaccg
    13081 gccacgacac gcgctcccgg tcccaggcgt cggccagctg ctgcggagac cacgagggcg
    13141 ggatggcggc ggccgcggcg cggccgccgg ggtgcgtcac ggccgggcgc gcggtcgcgc
    13201 cgcatccggc tgcgagcgcg atcgcggcca ggatcgccgt caacgcgccg gcggagccgc
    13261 cccggctccc gtagcgcgga gtgcgggggc cgttcatcct catacgccca gccatgttag
    13321 cagcccgcgc ccggggccgc gaaccgccgc cagcggggga cgggccccgg gtctgccggc
    13381 atcgggagga gcggactggc gggcttctgt ttagaatgag gcatgatcga ttccttgacg
    13441 cccgacggac cttctgctga tctccaccag cttcaccacg cgctccgcct gcagatcggc
    13501 aagccgccgg aagcgtgggg cgccgacgac ctcgccgcct tcgtgcgcga tcggcgcgtg
    13561 cggctgctct cgctcatgca cgtcggcggg gacggctggc tgaagacgct ggacttcgtt
    13621 ccccgcgact cggagcacct cgtggacgtg ctgctcggag gagagcgggc cgacggctcg
    13681 agcttgttcg gcgacctcgg cattccttcc ggcgcgtcgg acatcatgct gcgcccgagg
    13741 ctctcgacgg cgtttctcga tccgttctcc agcgagcctt cgctggcgct gctctgcagc
    13801 cattacaacc ggcacggcgc gccgctcgcc gagtcgccgg acacgctggt ccggtcggcc
    13861 taccgccggc tcgtcacggc caccggcgtc gagttgcacg cgctcggcga gatcgagtac
    13921 ttccttggca cgagatccgg cgaggcgtcc gcctacggga cctccgagcg gggctatcac
    13981 gccagctcgc cgttcgtgtt cggcgaggcg ctgcggcgca cggccctcgt gcgcctggcc
    14041 gagatgggcg tgccggtgaa gtacgggcat gccgaggtcg gatacgtcga ggccgaagcg
    14101 gccgacggcc gggcctggga gcagcatgag atcgagctgt ggctgcgccc gctgcccgag
    14161 gccgccgacg cggtcgtgct gacgcagtgg gtcctccgca atctcgccca gcgggcgggg
    14221 atgctgtgca gcttcgcgcc gatggcccga aaggggcacg ccggcagcgg gctgcacttc
    14281 cacttcgcgc ccctgaagga gcaggcgtac ctcccgcata ccggcgccga cggcggcctg
    14341 gcgccggagg cgcagtggct cattggcggg ctcgtcacgc acggggccgc catgatggct
    14401 ttcggcaacc gtgaccgcga ctcgttcgtc cgcctcagtc aggccaagga ggcaccgaac
    14461 gccgtcacct ggggccgtta caaccgcagg gcgctcgtca ggatcccgat catccccgtc
    14521 gatcaggccg gccggcagac gagtcccggt acggtcgagt tcaggctgcc cgacggctcg
    14581 gcacacccgc atctgctgct ggccggcatc gcccaggcct tcgtggacgg ccatgccatg
    14641 acggatctgg acgcccggct ggcgcgcacg gccgtcacgg cccacggcca cgctgcctcc
    14701 gagaaccgcc tgcctctcgc gtttcccgag gtcgcggacg ccctggcggc gagccggtcg
    14761 atgctggagg ctggcggcgt gttcacggcg aacctgctgg atcgcgtcat cgcgctgctg
    14821 cgggcacggg actgaagggg tcgggtcggt ttttgcaaac gacaattttg aatcacggaa
    14881 ttgtcgtttg caaaaaccga cccgacccct tcacgctcct caacagcagc gccaggaccg
    14941 ccacggtcac aactgcactc ccaatcagaa acgccagggg tgcgccttcg gcgccgatct
    15001 tcgcccgcac cgacacggtc atgccgacga gcgcagcgtc catcaccacg gcaaatgcga
    15061 gcatcgtcgg aacgagccag tagcctgacg ggcggcggcg caacagcagc aggccgccga
    15121 cgatgaaagc gggtagcacg acgcccaggt cgagcacctc gaccgggttc gtgatcagcc
    15181 ccgcctcacg cgcgctcctc aaaccggttg ctccggccag cgccggaacg atctcggcaa
    15241 gccagagcgc gccaaacatc acgccgagca gcaccgacac ggcgccggcc ggcctgatgg
    15301 gcgcgtccgc ctcaaaccat ccccggacat cgccggcgtt gagcgcgcac acctgcgcga
    15361 ccagcgcgta gaaagacagc ccgagcgccg ccgtgtagac gagaaagagg ggtccgaaat
    15421 gcaggaagaa ggcgtacacg accatcgagt agagcacgta cgccagcatg cccgcgagga
    15481 cgagcgacgc gacgcgggag ccccgcagga cgccatgcgc cgcgagggac aggagcggcg
    15541 ccacaaggac caggtccacc caatcctggc cgacgccctg cgcggcccag cgcggatgcg
    15601 cctctgcgta gacgccggga aggagaatgc cgccgagggc cgccaccgcc agcagggcgg
    15661 cgagcgggaa ggccagacac gcaggaaggc gttcgcgcgc ggtcacgaga gactcctatt
    15721 gcggggaccc cggcggcgcc gggggctgcc ctggatcggg actctggaac ggctgggcct
    15781 gctgaaccgg ctgcacgggc accacgcgcg cggggatctc gctcacgagc gcctgcgccg
    15841 caattcgcca gcgtcccgca gcgtccaggc gccagtgggt gacgtagttg ccgcgatcct
    15901 gcgaagacgg ccttcccggt ggagccgaag catcggccgc gaactggatt gtgtacttgc
    15961 cgcgctcgat ggccacgggc ccgctggtct cgaggaggct cgtcgcgagg ctgacttccc
    16021 agtgccccca tccgaacgcc agctgccagt agcgttgaat gtcgtcggtg ccgacgcgag
    16081 cggtggtatg aggcatcatc tgccatccgt cctcggtgaa cgcggcggcg acgcactcgt
    16141 actcgcccgc gttccagcag tcgaccagtt cttcgttcac cgcatcgatg tccgcccgca
    16201 cgtccgagac ctcgggcacc ttcttgacat tcgtgcaggc gccagcgagc acgagcgctg
    16261 cgatcaccgg gacggctcga ccatccatgg cgtcctcctg ccaactccgt gcggccgggg
    16321 ccggccgcgt ccccgcgcac catcgccgac gcgttcgcgc tctgacaagc cggcccggga
    16381 gcgcaggcgc gggcccgctg tcaggccccc gcagggcctc agaactcgac gcgagccgac
    16441 agaccggttc ctgccgccca gtaaggcgag cctgccgcat agcaggccac gatattatcg
    16501 ccgcgcgtcc aggccctgag ccggagcggg ggcacgaacg ccacatgccg gccgccgccg
    16561 cagacgtcta cgccgccgag cccggccgcg ctctgaagcg ccgggaagcg caccctgggg
    16621 gcgacgctca gcgcctggtt cggcgccagg cggggccggt acgtttccgt ctgcgactac
    16681 cggtcgtgcc agatcgcgcc aatcccggca agcggctcga tgtccgcgcg cctgccgggc
    16741 ctgaggtgca gagggaggag caggccgagc gcgcggcgaa gacgccgcct gccatcctcc
    16801 accccacggc tgtcccgcct ggcgccgcga tgtagatctg aggctccccg tcagccgcgc
    16861 cagcctggcg gacggccgcc acaccggccc ggcgcctcgg tccgccgccg caagccatcg
    16921 tacgcccccg cgttgcggcc ggcaagaggc cgtcccgacg ccgcctgcgc aagcgaagcc
    16981 cccgggggct tctggtagac tgaggggggc ccgcttctcc ccagggagcc actcatgtcg
    17041 gttgcctacg actacaaggt ccggaccgag tacaagggcg acgtgccgag gctgcgcgcc
    17101 gcccacacgg tcgtcgccga gttcccgttc caggccagcc tgccgatcag gggctacacc
    17161 cggcgcacac tgttcatcga cctggacacg atgtccgtgc gggagaagcc cgtcaccgag
    17221 gagatgatcg acatcttcac cggcggccgc ggcttcggca tgtactacct gtggcacgca
    17281 atcacgcccg agacgaagtg gaacgacccg gcgaacgaga tcatcatcag cccgggtccg
    17341 attgcgggca acccgcagta cgcggggtcg ggcaagtcgc tcgtcgtcac gatctcgccg
    17401 atgacgggca tccccatcga ctgcaacgtc ggaggctact tcggcccgct cttgaagttc
    17461 tgcggcttcg acgccctcga gatccggggc aaggcgaagg cggacaccgt catcttcatc
    17521 gacggtccga gcgggctgat caggctcgag gaggcgcccg aagaaacgcc tgacagccac
    17581 gtcgccgccg aggtgttcac gcacatgtac gccgccgacg cgaaggatct ccagaacgtg
    17641 tcggtggtgt cggcgggcaa ggccgccgag acggcctaca tgggctgcct gaacttctcc
    17701 tggtacgatc ccaagcgcgg agcggtccgg ctcaagcagg ccggccgagg cggcacaggc
    17761 acggtgttcc gggacaagcg cttgaaggcg ctcgtggtcc gcggccccaa gatgggcccg
    17821 aacctcaaca acgccgcgga cctgccgtcg gtcatcaggg tcggcaggat catcaaccag
    17881 gagatccacg atcacgacga cgagcagtgc cgcatgaggc ggcagggcac cgcgcacctg
    17941 gtcgagatca tggacgcgta cgacctcctg cccgtccaca acttccagta cggcaagcac
    18001 ctggacacgc accggatcga ctcgcaggtg tgggacaagc ggttcacgca ggcggttccg
    18061 gaccgctgct ggtacggctg cgggatggcc tgctcgaagg gcgccgacgg ccacatcgtc
    18121 cgcacgggcc cgtacaaggg ccacatcgtg acggtggacg ggcccgagta cgagaacgtg
    18181 ggcgggctcg cctcgaactg cggcctgttc gatccggact ggctgctcga ggtgaacttc
    18241 tactgcgata cctacggcat cgacaccatc tcctacggca ccacctgcgc gttcatcatg
    18301 gagtgctggg agaagggcat cctgaacgcg gagcgcaccg gcggcatcga cctctcctgg
    18361 ggcaacggcg agtcggaggt cgagctcctg caccagatgt ggcgcggcga ggggttcggc
    18421 gtccacgtgg gcaagggcgt gcggtacctc caggacctgt tcatcgagaa cggctggggc
    18481 gacccgcagt tcatccgcga catcgggatg cagatcaagg ggctcgagat ctcccagtac
    18541 atgtccaagg agtcgctggc gcagcagggc ggctacgcgc tggccaacaa gggcccgcaa
    18601 cacgacgagg cgtggctgat cttcatggac atggtgaaca accagatccc gacgttcgag
    18661 gacaaggccg aggcgctgca ctacttcccc ttgttccgaa cgtggttcgg cctgcagggg
    18721 ctctgcaagc tgccgtggaa cgacatcgag ccgccggaca accacgagaa gtaccccggg
    18781 ccggaggccg ccaaggtgcc ggcgcacgtg gacaactacc tcgcgctcta caccggcatc
    18841 acgggcaggc cgctcgaccg tgcgagcctc ctcgagcagt ccgagcgggt ctacaacttc
    18901 cagcgcgtgt tcaacctccg gatgggccag ggcgtccggg ccttcgacat gccgccttac
    18961 cgcgccatgg ggcccgtgac gaaggcggag tacgagtcgc gcgcggagcg ctacgacgcc
    19021 cagctcaagg acatcgtcgg catcgacccc gccgggaaga gcaccgagga gaagatggcg
    19081 ctccaccggc agtggcgcac ggaccgctac aacaggctcg tggacgccgt ctacaaacgc
    19141 cgcggctgga cgctgaacgg cgtgccgacg ctggagacgc tgaagaagct gcggatcgac
    19201 ttcccggagg tggtggaggt cgtgaaggcg caccaggagt agcggccggg ggcgcgtggc
    19261 cgggccctcg tcggtctcag cgccacgctg ggcacgccag ccgcgcggcg agagccgcgc
    19321 cggatcagcg cccggcgcct ggtctccggc ccctgtcaga cgcgggaacc cgatgcttcg
    19381 cgtgaacgac acgccgctcg agttcgaggc ggggatgacc gtgcgggaca tcctcaggcg
    19441 ccggaactac gtctggcgca tgatagccgt ctacgtgaac ggcgaactcg tgccccgcac
    19501 gacgtacgac acgcggcagg tgcccgacgg cgccgacgtc aaggtcatcc atcagatcgc
    19561 cgggggctga gccgcgcccg gggttgggcc tcgatttccg gatctgaccc cggttttccg
    19621 gatctgaccc cggttcactg caccgaggcc ccggcgcgcg cgcattgcaa tcgccgggcg
    19681 gcacgccacg cgccgaggta agatggccgc gaggacacca gcatggcgca gctgaaggtc
    19741 ggcgacaggg ccccggcgtt cgcgctggag gatcagcggg ggcggacggt caggctgtcc
    19801 gacttcaagg gacggaagct tctcgtctac ttctatccca aggccaacac gccgggatgc
    19861 accgcccagt cgtgcagcgt gcgcgacgcg cggacggact tcgccaggaa gggcgtcgct
    19921 gtcatcggcg tcagccccga caagccgtcg gctcaaagga ggttcgacga gaaacacggc
    19981 ttgggcttcc cgcttctcgc ggacactgac cacgtcatgg ccgaggccta cggcgtctgg
    20041 ggcgagaagt cgatgtacgg caggacgtac ttcggcatcg tcaggtcgtc gttcctcatc
    20101 gacgagcgcg ggcggatcgc cggcgcctgg tacaaggtct cgccggccgg caccgttccc
    20161 gaagccctga acgcactcgg ctgacgcgca caggcggggc caggtccgcg gatttccgga
    20221 tctgacccca acccccaatc agtgcgccca ggacagggcg ccgtcctcgg tgacgatgag
    20281 cagctcgccg cccttgatgg cgatccctga cggcttcgac aggccgggaa cgacgcgcgc
    20341 gtccgcgacg gtccgtctca ccggatcgac gacgaggagc gtgctgtagg ccgcgctcag
    20401 gacgtagagc cgcccctcgt ggaactcggc ggccgtcgga tagaagccgt ccagcgatcc
    20461 cgcgccgtcc ttgaaggcca gcgggccccc ctcggccagc gccgggctga actcctcagc
    20521 gagcgtcatg tcgcgccggt cgaaccgcga gaccacgagc cgctttgacg ccgcgttcgg
    20581 gacggtcacg gtgtagagcg cggatgccgc ggggtcgaac gccgccgcca tgacatacat
    20641 catcttcgcc cttaccgtcg tcaggcggga tcgcgaggct tcctcgaacg cgccgggcga
    20701 ctcgaggaag tagcggtagt tcgccgccgc atccgcctgg tcgctctcgc gcaggacgac
    20761 gtagctcttg ttctccgcca tcgcgaggac ggtgcggctg tcgaggaacg ccgccgccga
    20821 gaagcgcgcg agatcgaccg agtacccgac atcgacgacc gtgtgccgga tgacgcggcc
    20881 gagcgtgccg tccgtcaggt acacgccgtt gtgcgtcgtg aggaggaacc tgtcggacgc
    20941 cgcgtcgtat gccaggtccg tcaccgggcc gttgagcggc agggccagct tcgcgcgccg
    21001 cgacacggtc atcgcgggga cgcccgccag ggggcttccg gccgggtcgg cgggcgcgag
    21061 cgccacgtcg ggcttctcga tcgcccatcg gcctcgcagc gagacgggcg acggccgcca
    21121 ctcctcggtc gaccagaccc agttgcgggg attgaaggag aagcggaccg ggtcgccctg
    21181 gcccatgaag ggcggcggcc cggtgctcgc gaacgcctgg accatgttcg cggccaccgc
    21241 cagcaggaac agccacagcg cggctcgccc gggcgggcgc agctcgcgcg ggccctcgcc
    21301 cgccgcctcg ccgtccttca cggccagcag catggcgccc atcatcacga ggcacaccca
    21361 gaagatgaac agcgaccacg tgtaggtgtg cgcgcccagg atctccgcgc tgaacccctg
    21421 gcccacgtcg cgcgcgaagt gcagcgacga gtggcgaagg cccatgtaga cgccgaacgc
    21481 cgagatgagc acgcccatgc cgatgtagcg cgggcggggg ccgtagcgga cgatgaggag
    21541 gcccgtcagc gcgacgagca ccatcgccgt gcgctgggcc cagcagagga cgcacggcga
    21601 gtcgccgtgg acgaagccca ggacgaagac ggcgacgccg atgggcccga tgaggagggc
    21661 cagcacggcg gccgtcagga tcgtgtagag cgtgttccgg ttcatgggga gctctcctgc
    21721 ccgggcccgg gccgtcagta gttcaaccct gggaggatcg agcccctggc gccgatcagg
    21781 aagagcgcgg ccatgagcag cgaactcgcg acgagcagcc cgaaggcgag ccgctcgcgt
    21841 tcggggcggc gcaggacgag ccagccggcc gcgaagatca ggatgaagtt gagcagttcc
    21901 acggctggat catgccagag aacgcgtgcc gctgagaaca gggctcgcgc ggccggatgt
    21961 ccatcgcgcg cgggagccgg ccccgcgccg cgtcgcctgc tcccgccgcg cgcccccgcg
    22021 ccccgcgcgc ctacccgtgc gcgtgccggc gctcctcgat cagccgctgc accacctccg
    22081 gctcggcgag cgtggacgtg tcgcccaggc cgtcgtattc cgacgcggcg accttccgca
    22141 ggatgcgccg catgatcttg ccgctccgcg tcttcggcag gcctgaggcg acgtggatga
    22201 tgtcgggcgt cgcgaaggcg ccgatcgcgt tccgcacctg ctgcttcagc gcgccgacca
    22261 gctgctcggg gtcgccttcc gcgaagtcgc tgtggagcac cacgtagcag tagatgcccg
    22321 tccccttgat ctcgtgcgga tagccgacca ccgccgcctc ggccaccgcg tcgtgcgcca
    22381 cgagggcgct ctcgatctcc gccgtgccga tccggtgccc cgacacgttc agcacgtcgt
    22441 cgatccggcc ggtgatccag tagtagccgt cctcgtcgcg ggtggcgccg tcgccggtga
    22501 agtagtagcc gggataggtg gtgaagtagg tgtcgcggaa gagccggtgg ttgccccaga
    22561 ccgtgcgggc ctggcccggc cacg
//
LOCUS       DAOSIF010000007        19780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_2996_length_19780_cov_4.721318, whole genome shotgun sequence.
ACCESSION   DAOSIF010000007 DAOSIF010000000
VERSION     DAOSIF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 19780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19780
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_19780_cov_4.721318"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLT35_00625"
     CDS             complement(<1..641)
                     /locus_tag="PLT35_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27755.1"
                     /translation="MPAPTLASRTEGGTSAFQRWFPVLPFFAYWIGTLLLFEFGPLVN
                     DPLHAETYVYLLTGLACFAAAYAAGLAGRRWLRPVQTLREAALAYAWLRWTAPAALLG
                     AAGLVLDRLLSGAGSFSRTLTETQYVREEFGWNTTWITTLSVAPYCLSLVSLAAYFVS
                     ERHRRLPGYIHAMIIAQVALLAYNAFLSANRGVFFWLLTYWLFHLFFVRGFGV"
     gene            complement(873..1766)
                     /locus_tag="PLT35_00630"
     CDS             complement(873..1766)
                     /locus_tag="PLT35_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HOG27756.1"
                     /translation="MKKHAFLIIAHQDPVLLQQLVSLLDDERNGIYLLVDSKSGLRVD
                     GLAPTRASLTLLPPAPVWWGGVSTICAELSLLRAAAAHRYHYYHLLSGADLPLVSQDC
                     LHARLADTDFEYVEFHEGARDFARWKVAYYHLLVETAPYRAWWAYRMVGRALIRLQAL
                     AGIDRTRGSRTCFHHGSAFFSITHDLALYVLARERWVRERFRHTVTCDEVFLQTLAMR
                     SPFRGKLAGEHGIKPANLRLIDWATKRRHRNSPYVFRMPDYARLRDASETCLFARKFS
                     RAVDAKIVDFVVHELRTRGSL"
     gene            2199..2414
                     /locus_tag="PLT35_00635"
     CDS             2199..2414
                     /locus_tag="PLT35_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27757.1"
                     /translation="MSEVLEVPLRAANPALAGRYERLITGSRGMRVVDLIRARVKAAA
                     SFAPRSAPGRQMRSDSPRRWDRAAGPS"
     gene            complement(2599..3522)
                     /locus_tag="PLT35_00640"
     CDS             complement(2599..3522)
                     /locus_tag="PLT35_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOG27758.1"
                     /translation="MSAQTLSPARQTRIVKTLPFVEALARRMAASMPHSIDIGDLVQD
                     GVMGLMDAVRRFDESRGIKFETFAERRVRGAMIDALRREAWPRGVRRVRRELEAAREE
                     LRRTMGCEPSMADLAAKVGSDEKRLSRTIVRINTIESTSPYSVGDGVDEANMPTALIP
                     SEPESPDKAFQRCETEERVRAAIASLPWRERKVVRLYYYNEATMKDIGAEIGVNESRV
                     SQLHARAIQRLRAALGPETDPKSAATIMRDAIAAFQQKPRMLKAQLATVPAPLAARAK
                     APAVPAWTRMPVAMAAEKPARKRIGQSQKPA"
     gene            complement(3814..4029)
                     /locus_tag="PLT35_00645"
     CDS             complement(3814..4029)
                     /locus_tag="PLT35_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27759.1"
                     /translation="MSADPAELRQLFHALNNQLGIILSHAELIEAKAQDERQKARAGQ
                     VVAAAVEALAAARQIRLAVEGASLPSP"
     gene            complement(4026..4241)
                     /locus_tag="PLT35_00650"
     CDS             complement(4026..4241)
                     /locus_tag="PLT35_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG27760.1"
                     /translation="MERKAVSIMKACALVGVSRRTIYNWITAGKVEYVRTAGGSIRIF
                     VDSLWRDPQMGQAALDGTFAMPYEAHR"
     gene            4431..4625
                     /locus_tag="PLT35_00655"
     CDS             4431..4625
                     /locus_tag="PLT35_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HOG27761.1"
                     /translation="MPKVRRGGFVFLAWKGDHGPRHVHVYRGGTLVLKWDLENGKAMK
                     GRATRRVLELIAALRREGLL"
     gene            4622..5116
                     /locus_tag="PLT35_00660"
     CDS             4622..5116
                     /locus_tag="PLT35_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG27762.1"
                     /translation="MKIRSVAFNNHRRDFTVKAGGSTYRLPYSRLEPGLARDDTVVSA
                     AVDPEIGSEGFSYRAASGHTGTVHMEQVLDYNRDPRYMRDALLYELTLAAQARLAQAG
                     LSRRELARRMATSPSQLYRLLNQTNTRKSVDRMLVLLEALDCDVRLSVRRRPTPRAVS
                     ALPA"
     gene            complement(5130..6224)
                     /locus_tag="PLT35_00665"
     CDS             complement(5130..6224)
                     /locus_tag="PLT35_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27763.1"
                     /translation="MPPRFAFWTIILDGAPTSFRTRERDEILPLFNQLKAKNPTAELK
                     WFSGGRLWDSPEQAKEARHLERVRTFREERRKQERDARERTLGPGGESGRAPEREAAR
                     GEDPLAEFRRQHGRPADGSDDPSTRRASAPAVGRGPSIRSKKRRRRWSRESAPAEPGV
                     FGPVDSAPGPPKAERRGKDWRPGGEHKDPRDKYKLPPGAKRKRWKERHLGPRGRKPPH
                     PSFETRAEADLPREDRRREARPFSDRPRGDRPQGDRPFGDRPRGDKPFGQKPFGSKPF
                     GGKPKGGRPFGTKPSGSKPAGPGGPRRFGSKSSGGPGGYGKKPGAPGRGPGPGPGKPS
                     SPRKPFGGRPSGPRSFDRRPPRDRNRRGPK"
     gene            complement(6347..7231)
                     /gene="rfbA"
                     /locus_tag="PLT35_00670"
     CDS             complement(6347..7231)
                     /gene="rfbA"
                     /locus_tag="PLT35_00670"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOG27764.1"
                     /translation="MSTRKGIILAGGAGTRLYPVTQGVSKQLLPVYDKPMIYYPLSTL
                     MLAGIRDILVISTPQDTPRFEHLLADGSQWGLSISYAVQPEPNGIAQAFVIGRAFVVG
                     GPSTLVLGDNIFYGDSLQEILANAGARDEGATVFAYHVDDPERYGVVGFDAAGRATSI
                     EEKPARPKSSYAVTGLYFYDSQVADLAASLTPSARGELEITDLNRLYLDRGQLAVEKM
                     GRGYAWLDTGTHESLLEAGLFIQTIERRQGLKIACPEEIAFRQGFIGAAQVERLADAL
                     GRSNYANYLRRLLEEARS"
     gene            complement(7336..7725)
                     /locus_tag="PLT35_00675"
     CDS             complement(7336..7725)
                     /locus_tag="PLT35_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG27765.1"
                     /translation="MPIYEYQCRSCRGKTTALVLVRERESEVRCSRCGGADLEKLWSR
                     FASPKSEEARLDALADPAALAGIDENDPKSVASFMKKMGQEMGEDVGDIEQAMEEEMA
                     GGAAGADESGGPDDGGPVPGGSFSDDL"
     gene            complement(7778..8761)
                     /gene="dusB"
                     /locus_tag="PLT35_00680"
     CDS             complement(7778..8761)
                     /gene="dusB"
                     /locus_tag="PLT35_00680"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOG27766.1"
                     /translation="MPPFASPLLGLAPMAGLTDSAFRRLVKRHGACGHVVSEMVSSEA
                     LVRGLGRALEYLAFTEEERPVSIQIFGGDPARMAEAARLVEARGADGVDVNMGCPVPK
                     IARHSAGCGLMREPAHAAAIVSAMARAVAIPVTVKMRAGWSAREVNAPEIARRVADAG
                     AAAVTVHGRTAEQAYRGQADWDLVARVAQAVSIPVFGSGDLMDAQQIVDRLRTTPVAG
                     VLVGRGILHNPWLIAQAADELTGRPARVVAAGDRRRFLLEYIDLLAAEAPGAYRPDVH
                     DRWVVNKLRALCGYYTKGFEGGPRLRAAVNQAPSVAALRALIEEFFGAPAA"
     gene            complement(8768..9382)
                     /locus_tag="PLT35_00685"
     CDS             complement(8768..9382)
                     /locus_tag="PLT35_00685"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOG27767.1"
                     /translation="MTDHSHTPPAGSRAAGGSSAPAPQSVGPAFGVERAASAIDVVEA
                     PWYAVWTRSRAEKAVADQLARKDIEVFLPTIQRWSRWKDRRKRIDWPLFPGYCFARFD
                     LTAILDVLKSAGVATVVSFDGKPAPIPHQEVAAIQRLVETELQFDPCPLLHEGDLVEV
                     VRGPLKGVVGRLVRKGPKTKLVLSITMINRAVAVVFDAADVKKY"
     gene            complement(9414..10505)
                     /locus_tag="PLT35_00690"
     CDS             complement(9414..10505)
                     /locus_tag="PLT35_00690"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG27768.1"
                     /translation="MRHTILTVLAVVLIAAPSAAQAPPAGPPAGAGGPPQKVELLEQI
                     LVKVNGDIFTKTDLEARQIASLRQRAGEMSDEELRQAIAEITPGILVDAVDELLLLQR
                     GKELGYKVTEEQFKRVIENIRKENKLDTDEAFESALKHEGLTLAELRKNLEKQMVINQ
                     VQQVEVLGRIGISESEARTYYDANKNEFTTPATLTLRELLVTVPTDGVSVNVAADEEA
                     KAKAEQILARARAGEPFAALVAQFSEAPSKAGGGLVGPLSPDELNPAIRKLLEPLKPG
                     EITEVFRAANGYALLQVETMSTPEVRSFEDARDAIAQKVYEGKRLTEVQKYMRKLRGE
                     AIIEWKNADMEKLWMARTSPETAPKSPGH"
     gene            complement(10616..14278)
                     /gene="mfd"
                     /locus_tag="PLT35_00695"
     CDS             complement(10616..14278)
                     /gene="mfd"
                     /locus_tag="PLT35_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOG27769.1"
                     /translation="MPSLRLQELLRAARARAGFVREAKRGRVRVAGLTPAAKAFWIAG
                     AASDDPRRQLVLAVVASDRDVEQIVGDVRFFLGGLEGASAAELERTVLPFPSPEVDPY
                     RGLAPHLRIASARARALHAAATGLARVVVASAAALLPRVSPPDAIVALALDLVPGGEI
                     DTGRLADTLVDAGFTRRDPVDDHGEFCIRGGVVDVFPAGDDLPARIELVGDTIETIRR
                     YAPETQRSVETIDRLQIVPLGDVAGGGWPRVPVFDYLAGPRRPVLFALEPDEIRARIE
                     QHLAQIAASYAAAREAASRAPAGFARAQAPEEAAGRSGLPAPESILVGWPDVERHLAG
                     ADVLEELGLEAPASDAPGPHPHDADSAGGSPQAPGPRSPAPACSQPALSFANRLPDWI
                     AEVRAARAAGDTVVFAAATHGRAERLVEWLADYELRGAQIDRGDDAFAASVLITVGAL
                     SRGFRLPGAGFRIYAETDLFEEERRTRDRTTRGAARAFLSDLRDLKVGDLVVHVDNGI
                     GEFVGLKEMTVAPNETLEFLELRYAGDDKLFVPVARLDLVQKYLGGARPALDRLGGTT
                     WEKAKTRVKKAMRDMAEELLRLYAARKAVGGHAFAADTHWQAEFDGAFPFDLTADQVT
                     AIEDIKRDMESQAPMDRLLCGDVGYGKTEVSMRAAFKAVMDGKQVAYLAPTTVLAFQH
                     AKTLQERFSGFPVRVDLLSRFRSRQEQKATLADLAAGRVDIVVGTHRLLSRDVVFRDL
                     GLLVVDEEQRFGVAHKERIKQIRRTVDALTMTATPIPRTLNMSLVGIRDMSIIETPPK
                     DRLAIQTHVVKFDQGLIVRAVRHELARGGQVYFVHNRVESIYSIGSLLARLVPEARIV
                     VGHGQMGERDLEQAMIDFVAGRFDILLATTIVENGLDIPNVNTILINRADRYGLAQLY
                     QLRGRVGRSDRPAYAYLLVPPEASLSAVAKKRLAAIREFSDLGSGFRVAALDLEIRGA
                     GNLLGGEQSGHIDAVGFEMYMKLLEQAVRELKGEEVEEDVRAAVNLGIDIRIDEEYVP
                     DMAQRLVVYRKIAAARSEDEIGEVLEDVKDRYGPLRDSVLNLAAYGRIRVLADRVGVE
                     SIDRQGSAVVLKFRDGAKPPARTPDPARVLGLLRRRPDVQFVAPSSLRLSLDRGQAAE
                     FAAAEGPRPRISGPEPGGGLSRRAPSPSWWTARATTGAVTPGFSKQEILKPRKEDPRA
                     EGGLFERVSGLLEQLG"
     gene            complement(14361..15887)
                     /locus_tag="PLT35_00700"
     CDS             complement(14361..15887)
                     /locus_tag="PLT35_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27770.1"
                     /translation="MLSTADRRSALVALAGAALALWANAGHGPGLSPESAGYVSAARS
                     LAGLRGLLWYDGGPLLAQPPLYPAVLACAGLLTASDPLRAAGPVSVLLYLAAVAVAGA
                     IASRRLHLAPGPAMFAAAAAAAAYPLFYVSAMTWPDLLLAALVGAALFAGDAYAARRT
                     RGALAALAAFAAAASLARYSGAAVIAWAAIVVLAYGPGRVLARIGRAALFAAAASVPL
                     AAWLAARPGVSAALLQSRPGASAVDVARNLEAAATGVVAWYAPVGRVGIAGPLAVMLL
                     VAAAVFVVIRGSGRPGGHDAPPAGTVAGARIWVTAALALIFALAVVATTEAGARAQIS
                     DRALAPIAIPVAVLGACLLDLLLRARPAGRIVVLRKSVVAALVAGMLAWPVVRTVRAA
                     SWFSETGVGIAERGWQDSATAGYLLAHLDDLRAAPVYSNAPDAVYLLTGLRARWSPEK
                     GPGPALQAGLWPPEGRAVLVWFSRRQTESLWPLEELSRVSRLQDLAILNDGRVLLARR
                     "
     gene            complement(15888..16358)
                     /gene="rfaE2"
                     /locus_tag="PLT35_00705"
     CDS             complement(15888..16358)
                     /gene="rfaE2"
                     /locus_tag="PLT35_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOG27771.1"
                     /translation="MTRRVASREEAVAWREALGREGKRVVFTNGVFDILHPGHVRYLT
                     RARESGDALVVAVNSDRSVRANKGPTRPVHPEAERAEVVAALGVVDLAVIFDEDTPHD
                     IIAAIQPDVLVKGADWPADRIVGRDIVEARGGVVIRVPVEQGHSTTAVIAKLKA"
     gene            complement(16355..17719)
                     /locus_tag="PLT35_00710"
     CDS             complement(16355..17719)
                     /locus_tag="PLT35_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG27772.1"
                     /translation="MMPAPARAIDVVNVTKTYRRYARRKQFATLKSAILSGTLVGDLR
                     PEDSFHALRGVSFGVRAGSTFGIVGRNGSGKSTALKLVAGITKPTTGTVSVRGRISAL
                     IELGAGFHPEISGRENVFINGIMLGLTKREITRRFDEIVEFAELEDFIDAPVKTYSSG
                     MYMRLGFAVAINVDPDVLLIDEVLAVGDEAFTHKCLDKFAEYRRRNKTVLLVTHALNL
                     VERFCDEALWLDDGRARAVGDPRRVVSAYITDVEKQEERHMAEADAKAREAVERHDAA
                     PAEPESRIPRPESPRAAPPDMFQAAEGRWGSREGEITDVRLVGEDGQPARVFYSGERV
                     AIRLSLKAAAPLDDFVIGIGIFNADGVCAFGTNTNIEDLQGERLEGEGEAVVTIDALD
                     LVAGTYKLDVALHKLDGYPYDYHRLLYSFRVKSRTRDAGIYRPRHSWGFSGGVRVSER
                     VAGS"
     gene            complement(17716..18507)
                     /locus_tag="PLT35_00715"
     CDS             complement(17716..18507)
                     /locus_tag="PLT35_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG27773.1"
                     /translation="MLKNLRGLLKYRQLIQSMVARELKARYRGSVLGFFWSFINPLLL
                     LLVYTFVFSYVLPARQPNLDPYALFLFCGILPWTWFSSSILESANVLISGGNLLKKVM
                     FPAEVLPFVTVLANMVHFFLGLPILVGFLIYYRAPLQASELAWFPVVVLVQLVLTLGL
                     ALIVSALTVHFRDLKDILSNLVTFWFFATPIIYSMQDAPALAKRFLNGNPFTHLAISY
                     QEILFYQGDFGHWKWLLALGAGSVALFLAGYYLFDRLRDTFAEEV"
     gene            complement(18501..19340)
                     /locus_tag="PLT35_00720"
     CDS             complement(18501..19340)
                     /locus_tag="PLT35_00720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG27774.1"
                     /translation="MLSASLTDPGLRRPLNEDALVARPGLGLFAVFDGMGGYAAGEVA
                     SRVAAGALEDFIQHTDGVTADFAWPFEYRPERGLDGNRLACGLLLANREIRLRMEDEP
                     ALRGMATTAVCLLLGTRDLALGAGGQPPSRLAPSAQPLAPSIPALVAHVGDSRAYLWR
                     GGALDQITRDHSWVEEQVALGVLGEDEARVHPLRNLVTRALAGAEEPRVELTPVSLEP
                     GDRAILCSDGLTSPLTNEAVAEILARTPPGRPEEACRALVEAAIAAGGPDNITVVVVE
                     VAC"
     gene            complement(19348..>19780)
                     /locus_tag="PLT35_00725"
     CDS             complement(19348..>19780)
                     /locus_tag="PLT35_00725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOG27775.1"
                     /translation="MPLPPRLVLEVVTPDHAVVREEVDEVQLPGVAGYLGILPGHTPL
                     LTSLRVGQLWYRQGAEKRYLAIAFGFAEVQPDRVRLLAQVAERAEEIDVARAEERRRR
                     AETAVQAARSGSADIDLERARISLLKSIVRIQVAAKAKLRG"
BASE COUNT         2935 a         6977 c         6937 g         2931 t
ORIGIN      
        1 ctgacgccga agcccctcac gaagaacaag tggaacagcc aataggtcag caaccagaag
       61 aagacgcctc gattggccga caagaaggcg ttgtacgcca gaagcgccac ctgggcgatg
      121 atcatggcgt ggatgtagcc gggcaggcgt cggtgtctct cggaaacgaa gtacgcagcc
      181 agggaaacaa gggacaggca gtagggcgcg acgctcagcg tggtgatcca ggtcgtgttc
      241 cacccgaact cttcacgaac gtactgcgtt tctgtcagcg tgcggctgaa gctgcctgct
      301 ccgctcagga ggcgatccag caccagtccc gccgcgccga gcagggcggc tggagcagtc
      361 catcgcaacc atgcgtaggc cagcgccgct tcgcgaagcg tctggaccgg tcgcagccat
      421 cggcgccccg caagcccggc ggcataggcg gccgcgaagc acgcgagtcc ggtgaggagg
      481 tacacgtacg tttcggcatg gagggggtcg ttgacgagag ggccgaactc gaacagcagg
      541 agcgtgccaa tccagtaggc gaagaacggt aggactggga accaacgctg gaaagcactg
      601 gtcccgcctt cggttcgcga ggcgagggtg ggcgcaggca tgcttcgctg tgcggcctcg
      661 atgggtcttc agcggcggca aacacgcggg gtcagcccga ttccggctcg aaggcgaggc
      721 tccccgccgc gacgcttcac acgcaagggc tgtgcagtcg attcaacagg cgcacgggac
      781 gaggcaaggc acaactccgc cccgtgactt acaggccgtg ccgaaacgcc cacccttggg
      841 ccggcgaatc ggtcaatctt accccgaagc cgtcacagag agccgcgtgt tcgcagctcg
      901 tgcaccacga aatcgacgat cttcgcatcg actgcgcggc tgaacttccg agcgaacagg
      961 caggtctcgc tggcatcccg caaccgcgcg tagtccggca tccggaagac gtacggggag
     1021 ttccggtgcc gacgcttcgt ggcccagtcg atcaagcgga gattggccgg cttgatgccg
     1081 tgttccccgg cgagctttcc cctgaacggc gaccgcatgg ccagcgtctg gaggaacacc
     1141 tcgtcacagg tcacagtgtg ccgaaagcgt tcgcgcaccc accgttctcg cgcgaggacg
     1201 tacagcgcca ggtcgtgagt aattgagaag aacgcggagc cgtggtggaa gcatgtcctg
     1261 gagcccctgg ttctatcgat tccggccagc gcctgcaatc tgatgagcgc ccgccccacc
     1321 atgcggtaag cccaccacgc cctgtaggga gccgtctcaa ccaggaggtg gtagtaggct
     1381 accttccagc gggcaaagtc cctggctccc tcatggaact cgacgtactc gaagtccgtg
     1441 tccgcgagtc tcgcgtgcag acagtcctga ctcacgagcg gaagatccgc gccggacagc
     1501 aagtggtagt agtggtagcg atgcgcagcc gctgctctca ggagactcag ttcggcgcaa
     1561 atggtcgaga cgccgcccca ccacaccgga gccggcggaa gcagcgtgag cgatgcgcgc
     1621 gtcggggcga gcccgtcgac gcgaagcccg ctcttcgagt ccacaagcag atagatgccg
     1681 tttcgctcgt catcgagcag ggagacgagc tgctggagca acaccgggtc ctggtgggcg
     1741 atgatcagga acgcgtgctt cttcacggct cagggactcc cccggcaccc agcatcctat
     1801 cgggtgcgca tggcaggcgc ctgccgaggc ttgccgctaa gggccggtcc gcatctcgag
     1861 gaacgccgag tcagtccggt tgcgaaccac ctcgtggcgg gtagttatgc cacagccggc
     1921 agcaggcgtc caacgcactg ggtccgaccg gcgggcgtcg gaccagtcgc gtggcgcacc
     1981 gcccgactct gcgggggcgg tggcttcgcg cgcatcatga acccctcgac accagccagc
     2041 gagaacgcag cctcgccggg cgcgcaagtc ctgcagtgct ctcgcggacg gcgagctgca
     2101 gtcactgccg cccgcggacg gcagcgatag gcctggcgca acccctcttc ctcgaagccg
     2161 acctgggcga acgcctgctc gtcacctcgg ctctgacgat gtccgaagtg ctggaggtgc
     2221 cgctccgcgc cgcgaatcct gcgctcgccg ggcgctacga gcgcctcatc accggaagcc
     2281 gcggcatgcg cgtggtcgat ctcatccgcg cccgggtcaa ggcggccgcc agcttcgcgc
     2341 cgcggtcggc gccagggcgc cagatgcgct ccgactcgcc gcggcgctgg gatcgggctg
     2401 ccggaccttc ctgacgaacg gcaggcgcct gctggtgatc ccaggccagc gcgtgattca
     2461 actgtcgtcg cacgttgcgt gaaagcgcca ccggggaagg gcaccggcac gagcgcggcc
     2521 tcgctgggcc gcgcagaaaa ggatccgcgc caggaggctg ggtctatccg caagcgcaga
     2581 gcggcacgcc ggcacgcgtc aggccggctt ctggctctgg ccgatccgct tccgcgccgg
     2641 cttctccgcg gccatcgcca cgggcatccg cgtccaggcc ggaaccgccg gcgccttcgc
     2701 gcgggccgcg agcggcgcgg gcacggtcgc aagctgcgcc ttgagcatcc tgggcttctg
     2761 ctggaaggcc gcgatggcgt cgcgcatgat ggtcgcggcc gacttgggat cggtctccgg
     2821 gccgagggcg gcccgcaggc gctggatggc ccgcgcgtgg agctgcgaca cgcgcgactc
     2881 gttcaccccg atctccgcgc cgatgtcctt catcgtggct tcgttgtagt agtagaggcg
     2941 cacgaccttg cgctcgcgcc acgggagcga cgcgatggcg gcgcggacgc gctcctccgt
     3001 ctcgcagcgc tggaacgcct tgtcgggcga ctcgggctct gacgggatga gcgccgtcgg
     3061 catgttcgcc tcgtccaccc cgtcgccgac gctgtagggc gacgtcgatt cgatcgtgtt
     3121 gatccgcacg atcgtgcggc tgaggcgctt ctcgtcggac ccgactttgg ccgccaggtc
     3181 ggccatcgac ggctcgcagc ccatcgtgcg gcggagctcc tcgcgcgcgg cctcgagctc
     3241 gcggcgcacg cggcggacgc cgcgcggcca cgcctcgcgg cgcagcgcgt caatcatcgc
     3301 cccgcggacg cggcgctcgg caaacgtctc gaacttgatg ccccggctct cgtcgaaccg
     3361 ccggacggcg tccatgagcc ccatcactcc gtcctggacg aggtcgccga tgtcgatcga
     3421 gtgcggcatc gaggcggcca tccgccgcgc gagcgcctcc acgaacggca gggtcttgac
     3481 gattcgggtc tgacgggctg gggagagtgt ctgagcgctc atcgagatct ccaggacgta
     3541 gtcggcgtgt ccgtacgcga ccgtcattga cgcaacgcgg gtgccacgat tcgtcaaaga
     3601 cgcgatggga tgcgccgtgc cgtgaagacg cccgcaattc gttgatattc aacgacttga
     3661 tgccgagttt cggagaagaa ccgtgcagcc gcgtgcagcg ctcgtggggc cgcggcgtca
     3721 aaaccatgac gggctggatt gcacaggatg tcaacggggt gacgaaaagg ggattgcggg
     3781 tcggaaaccg gaggtcggaa accagaggcc gtgctagggg ctcgggagag atgctccctc
     3841 caccgccagg cgaatctgac gggccgcggc gagcgcctcg acggcggcgg cgacgacctg
     3901 gccggcccgc gctttctgcc gctcatcctg cgccttggcc tcgatgagct cggcgtgaga
     3961 caggatgata ccgagctggt tgttcagcgc gtggaacagc tgacgcagct cggccggatc
     4021 cgcgctcacc ggtgcgcctc gtacggcatg gcgaaagtgc cgtcaagcgc cgcctgcccc
     4081 atctgcggat cgcgccagag cgagtccacg aagatccgga tcgacccgcc ggccgtcctc
     4141 acgtactcga ccttgcccgc ggtgatccag ttatagatgg tgcgccggct gacgccgacg
     4201 agcgcgcatg ctttcatgat cgaaacggct ttccgttcca cgagctgccc tccgtggaac
     4261 cgtatcggac agagtgggaa gaactgtaga gaagcggcac ggccattgcc tcgtctgtcc
     4321 ggcgtagggc ggtttcgggc cgcgagtacg caaccactgc catgcgtcgc aaccgcctgt
     4381 gtggcaggat cagccgcctt gataaacaga acctcttgtg gtaacacttt atgccgaagg
     4441 tccgccgcgg aggattcgtg ttcctagcct ggaaaggtga ccacggcccg cgtcacgttc
     4501 acgtctaccg cgggggaacg ctcgtgctga agtgggacct cgagaacggg aaggcgatga
     4561 agggcagggc aacgcgccgg gtgctggaat tgattgcggc gctgcggcgg gaggggctgt
     4621 tgtgaagatc cggtccgtgg cgttcaacaa ccatcgccgc gatttcaccg tcaaggcggg
     4681 cggatcgacc taccggttgc cgtactcaag gctggaaccc ggactcgccc gggacgacac
     4741 ggtcgtatcg gccgccgtcg atccggagat cggcagcgag ggcttcagtt accgggccgc
     4801 gtccgggcac acgggtaccg ttcacatgga gcaggtcctc gactacaaca gggacccgcg
     4861 ctacatgcgg gacgcgctgc tgtacgagtt gacgctggcc gcgcaggcgc gcctcgcaca
     4921 ggctggcctg agccggcggg aactagcccg gcgcatggcc acgtcgccgt cgcagttgta
     4981 ccgcctcctg aaccagacga acacccggaa gtcggtggac aggatgctgg tgctgctcga
     5041 ggcgctcgat tgcgacgtcc ggttgtcggt tcgccggcgt ccgacgccgc gggcggtatc
     5101 ggcccttccg gcctgaccgg cgctcggcgc tacttcggtc cgcggcggtt gcggtcgcgc
     5161 ggcgggcggc gatcgaacga acgcgggccg gacggtctac cgccgaaggg tttgcgcgga
     5221 gaggacggct tgcccggccc cggccccggc cccctgcctg gcgcgcctgg ctttttcccg
     5281 tagccgccgg ggccgccact ggatttcgat ccgaatcgcc gcggtccgcc tgggcctgcc
     5341 ggcttgctgc cggacggctt cgtgccgaat ggccggccgc ctttgggctt cccgccgaag
     5401 ggcttcgaac cgaacggctt ctggccgaac ggcttgtcgc cgcgcgggcg atcgccgaac
     5461 gggcggtcgc cttgtggacg gtcgccgcgc ggccgatcgc tgaacggccg agcctcacga
     5521 cgacgatcct cgcgcggcag atccgcttca gcccgagtct cgaaggacgg atggggaggc
     5581 ttccgaccgc ggggcccgag gtgccgctcc ttccaccgct tccgcttcgc tccgggcggc
     5641 agcttgtact tgtcgcgcgg gtccttgtgc tcgcctcccg gccgccagtc cttgccgcgg
     5701 cgctcggcct tcggcggccc gggggccgaa tccacgggtc cgaaaacccc gggctccgcc
     5761 ggcgccgact cgcggctcca ccgacgcctt ctcttcttcg aacgaatcga cggcccgcgt
     5821 ccgaccgctg gagcggacgc tctgcgggtc gaggggtcgt cgctgccgtc ggcaggccgg
     5881 ccgtgctggc gccggaactc agccagcgga tcttccccgc gcgcggcctc gcgctccggc
     5941 gcgcgcccgg actcgcctcc tggtccgagc gtccgctccc gtgcgtcccg ctcctgtttc
     6001 ctgcgctctt cgcggaacgt gcgaacgcgc tccaggtgcc gcgcttcctt ggcctgctcc
     6061 ggggagtccc acagcctgcc gcccgagaac cacttgagct ccgccgtcgg gttcttggcc
     6121 ttcaactggt tgaagagcgg caggatctcg tcccgttcgc gcgtgcggaa ggacgtgggc
     6181 gccccgtcga ggatgatcgt ccagaaggcg aagcggggag gcatggggtc tttctccagg
     6241 ggctggggag caggggctgg gggctggtca gactcgttgt ttctcagcac cctggccccc
     6301 gggcgtcgcg ccgcccgccc ccatgggcgt tttgtctctg acggtgctac gaacgtgctt
     6361 cctccagcag gcggcgcagg tagttcgcgt agttgctcct gccgagggcg tcggcgaggc
     6421 gctcgacctg tgccgcgccg atgaagccct ggcggaacgc gatctcctct gggcacgcga
     6481 tcttcaggcc ctgccggcgc tcgatcgtct ggatgaagag cccggcctcg agcagcgact
     6541 cgtgcgtgcc cgtgtcgagc cacgcatagc cgcgccccat cttctccacg gccagctgcc
     6601 cgcggtcgag atagaggcgg ttgaggtcgg tgatctccag ctcgccgcgc gccgacggcg
     6661 tcagcgacgc ggcgagatcg gccacctggc tgtcgtagaa gtagaggccg gtgacggcgt
     6721 aactcgactt ggggcgcgcc ggcttctcct cgatggacgt cgcgcggcct gccgcgtcga
     6781 acccaacgac gccgtagcgc tcgggatcgt cgacgtggta ggcgaacacc gtcgcgccct
     6841 cgtcgcgcgc gcccgcgttc gccaggatct cctgcaggct gtcgccgtag aagatattgt
     6901 ccccgagaac gagcgtcgac ggcccgccga cgacgaacgc gcggccgatg acgaacgcct
     6961 gcgcgatgcc gttcggctcg ggctgcacgg cgtagctgat cgagaggccc cactgcgatc
     7021 cgtcggccag gaggtgctcg aagcgcggcg tgtcctgcgg cgtcgagatg acgaggatgt
     7081 cgcggatgcc cgccagcatc agcgtgctga gcgggtagta gatcatcggc ttgtcgtaga
     7141 cgggcagcag ctgcttggag acgccctggg tgaccggata caggcgcgtc ccggcgccgc
     7201 ccgcgaggat gatgcctttg cgcgtgctca tggctggcac gattgtactg cgtgtcgggg
     7261 acccctggcg cggccgcccc gagcgcgtcc cgcacgccgc gggcggcgcg gccacgggca
     7321 gcgggtggcg ccgggctaca ggtcgtcgga gaacgagccg ccgggtaccg gcccgccatc
     7381 gtccgggccg ccgctctcgt ccgcgccggc ggcgccgccg gccatttcct cttccatggc
     7441 ctgctcgatg tcgccgacgt cctcccccat ttcctgcccc atcttcttca tgaagctcgc
     7501 cacgctcttc gggtcgttct cgtcgatgcc ggccagggct gccggatcgg caagcgcgtc
     7561 cagccgcgcc tcttccgact tgggcgacgc gaaccgcgac cagagcttct cgaggtccgc
     7621 gccgccgcag cgcgagcagc gcacctccga ctcccgctcg cggacgagca cgagagcggt
     7681 ggtcttcccg cgacacgaac ggcattgata ctcgtagatc ggcatgatcg gagccagggt
     7741 caagggacca ggcgctcggc gctggtcggt cacggtgtca ggctgcgggc gcgccgaaga
     7801 actcctcgat caatgcacgc agcgctgcca ccgacggggc ctggttgacg gcagcgcgca
     7861 gccgcggtcc gccttcgaat cctttcgtgt agtagccgca cagcgcgcgc agcttgttca
     7921 cgacccagcg atcgtggacg tcggggcggt aggcgccggg cgcctcggcc gccagcagat
     7981 caatgtactc cagcaggaac cgcctgcggt ctccggccgc cacaacccgg gccgggcgcc
     8041 ccgtcaactc gtcggccgcc tgcgcgatga gccacgggtt gtgcaggatg ccgcgtccga
     8101 ccagcacgcc ggccacgggc gtcgtgcgca gccggtcgac gatctgttgc gcgtccatca
     8161 ggtcgccgct gccgaacacg ggaatggaca cagcctgcgc cacgcgcgcc acgaggtccc
     8221 agtcggcctg tccccggtac gcctgctcgg cggtgcgccc gtgcacggtc accgccgccg
     8281 cgccggcgtc cgccacgcgc cgcgcgatct ccggcgcgtt cacctcgcgg gcgctccagc
     8341 ccgcgcgcat cttcacggtg acggggatcg ccacggcccg cgccatggcc gagacgatcg
     8401 cggcggcgtg cgcgggctcg cgcatcaggc cgcagcccgc gctgtgcctg gcgatcttcg
     8461 gcacggggca gcccatgttg acgtccacgc cgtccgcccc gcgcgcctcg acgaggcgcg
     8521 cggcctcggc catgcgcgcg ggatcgcccc cgaagatctg gatggagacg ggccgctcct
     8581 cctccgtgaa cgcgaggtac tccagcgcgc ggccgaggcc ccgcacgagc gcctccgacg
     8641 acaccatctc ggacaccacg tgcccgcacg ccccgtgccg cttcacgagg cgccggaacg
     8701 cgctgtcggt caggccggcc atcggagcga ggcccagcag gggggaggcg aagggtggca
     8761 cgccgcttca gtacttcttg acgtcggccg cgtcgaagac gacggcgacg gcgcggttga
     8821 tcatcgtgat agagaggacg agcttcgtct tcgggccctt gcgcacgagg cggccgacga
     8881 cccccttcag cgggcctcgc accacctcga cgagatcgcc ttcgtggagg agcgggcagg
     8941 ggtcgaactg cagctcagtc tcgacgaggc gctggatcgc cgcaacttcc tgatgcggaa
     9001 tgggcgccgg cttcccgtcg aacgacacca ccgtggccac gcctgcgctc ttcagcacgt
     9061 ccaggatcgc ggtgagatcg aacctggcga agcagtaccc cggaaagagc ggccagtcga
     9121 tccgcttcct ccggtctttc caccgcgacc atcgctggat cgtcggcagg aacacctcga
     9181 tgtccttgcg ggcgagctgg tcggccacgg ccttctccgc ccgcgaccgg gtccacaccg
     9241 cgtaccacgg cgcctcgacg acatcgatcg cagacgccgc gcgctcgacg ccgaaggccg
     9301 ggccgacgga ctgcggtgcc ggcgcggacg atccgccggc ggctcgcgac ccggccggcg
     9361 gcgtgtgcga gtggtcggtc atggcgtcac gttcgggcga gaccggcgcc gcgtcagtgc
     9421 ccgggcgatt tcggggcggt ctcgggcgac gtcctggcca tccagagctt ctccatgtcg
     9481 gcgttcttcc actcgatgat ggcctctccg cggagcttgc gcatgtactt ctggacttcc
     9541 gtcaggcgct tgccctcgta gaccttctgc gcgatggcgt cgcgggcgtc ctcgaagctc
     9601 ctcacttcgg gcgtgctcat ggtctcgacc tgcaggagcg cgtagccgtt cgcggcgcgg
     9661 aagacctcgg tgatctcgcc cggcttgagc ggctcgagga gcttccggat ggcgggattc
     9721 agctcgtcgg gcgacagcgg gccgaccagg ccgccgccgg ccttggacgg ggcctcggag
     9781 aactgggcca cgagcgcggc gaagggctcg ccggcgcggg cgcgcgccag aatctgctcc
     9841 gctttcgcct tggcctcctc gtctgcagcg acgttgacgg agacgccgtc ggtcggcacc
     9901 gtgacgagca gttcgcgcag ggtgagcgtc gccggcgtgg tgaattcgtt cttgttcgcg
     9961 tcgtagtacg tccgcgcctc ggactcggag atgccgattc ggcccaggac ctcgacctgc
    10021 tggacctggt tgatgaccat ctgcttctcg aggttcttgc gcagctcggc cagcgtcagt
    10081 ccctcatgct tcagggccga ctcgaaggcc tcgtcggtgt cgagcttgtt ctccttgcgg
    10141 atgttctcga tcacgcgctt gaactgctct tccgtgacct tgtagcccag ctccttgccg
    10201 cgctggagca ggagcagctc gtccaccgcg tcgaccagga tgcccggcgt gatttccgca
    10261 atcgcctgcc ggagctcctc gtccgacatc tcgcccgcgc gctggcgcag cgacgcgatc
    10321 tggcgggctt cgaggtcggt cttggtgaag atgtcgccgt tgaccttgac gaggatctgc
    10381 tcgagcagct cgaccttctg cggcggccct cccgcgccgg ccggcggccc ggcgggcggc
    10441 gcctgtgcgg ccgacggcgc cgcgatcaga accacggcga gcacggtcaa gatggtgtga
    10501 cgcatggggc gcttcctgcc aaacccaaac cgttattcta cctgagtttg gacgcagcca
    10561 gcgccccggc ggcctggccg cgcccgcacc gggcgccggg atcgttctcc cggcttcatc
    10621 ccagctgctc cagcaggccg cttacccgct cgaacaggcc cccttccgcc cgggggtcct
    10681 ccttgcgggg cttcaggatc tcctgcttgg agaagcccgg agtaacggcc cccgtcgtcg
    10741 cccgcgcggt ccaccaggac ggactcggcg ccctgcggga gaggccgcct ccgggctcgg
    10801 gtccggaaat ccgaggccgg ggcccttcag cggccgcgaa ttcggcagcc tgacccctgt
    10861 ccaggctgag gcgcagcgac gagggcgcca cgaactgcac gtcgggtcgg cgccgaagca
    10921 atccgagcac cctggccgga tccggcgttc gcgccggcgg cttcgctccg tcgcggaact
    10981 tcagcacgac cgcgctcccc tgccgatcga tggactcgac gcccacccgg tccgccagca
    11041 cgcggatccg cccgtaggcc gccaggttga gcaccgagtc ccgaagcggt ccgtaccggt
    11101 ccttcacgtc ctccagcacc tcgccgatct cgtcctcgct ccgcgccgcc gcgatcttcc
    11161 tgtacacgac gagccgctgg gccatgtcgg gcacgtactc ctcgtcgatg cggatgtcga
    11221 tgccgaggtt gacggcggcg cgcacgtcct cctcgacctc ctcccccttc agttcccgca
    11281 ccgcctgctc cagcagcttc atgtacatct cgaagccgac ggcgtcgatg tgcccgctct
    11341 gctcgccgcc gagcaggttc ccggcgcccc ggatctccag gtcgagcgcc gccacgcgga
    11401 agccgctgcc gaggtcgctg aactcgcgga tggccgccag gcgcttcttc gccacggccg
    11461 agagcgacgc ctccgggggc acgagcaggt acgcataggc cggccgatcg gatcggccca
    11521 cgcgcccgcg aagctggtag agctgcgcca ggccgtaccg gtccgcgcgg ttgatcagga
    11581 tcgtgttcac gttcgggatg tcgaggccgt tctcgacgat ggtcgtcgcg agcaggatgt
    11641 cgaacctccc cgccacgaag tcgatcatcg cctgctcgag gtcgcgttcg cccatctgcc
    11701 cgtggccgac gacgatccgc gcctcgggca cgaggcgggc gagcaggctt ccgatcgagt
    11761 agatcgactc cacccggttg tgcacgaagt agacctggcc gccgcgcgcc agctcgtgtc
    11821 gcacggccct gacaatcagg ccctgatcga acttcacgac gtgcgtctgg atcgccaggc
    11881 ggtccttggg cggcgtctcg atgatcgaca tgtcccggat cccgacgagc gacatgttga
    11941 gcgtccgcgg aatcggcgtc gccgtcatcg tcagcgcgtc cacggtgcgg cggatctgct
    12001 tgattctctc cttgtgcgcg acgccgaacc gctgctcttc gtccaccacg agcaggccca
    12061 ggtcgcggaa gacgacgtcc cgcgacagca ggcggtgcgt gccgaccacg atgtccaccc
    12121 tgccggcggc gaggtcggcg agcgtcgcct tctgctcctg ccgcgagcgg aagcggctga
    12181 gcaggtcgac gcgcacgggg aagccgctga agcgctcctg cagcgtcttc gcgtgctgga
    12241 acgccaggac cgtcgttggc gcgaggtacg cgacctgctt gccgtccatg acggccttga
    12301 aggcggcgcg catcgagacc tcggtcttcc cgtagcccac gtcgccgcac agcaggcggt
    12361 ccatgggcgc ctgcgactcc atgtcgcgct tgatgtcctc gatggccgtc acctggtcgg
    12421 ccgtgagatc gaaggggaac gcgccgtcga actccgcctg ccagtgcgtg tcggcggcga
    12481 aggcgtgccc gccgacggcc ttccgcgcgg cgtagagcct cagcagctcc tcggccatgt
    12541 cgcgcatggc cttcttcacg cgggtcttgg ccttctccca cgtcgtcccg ccgaggcgat
    12601 cgagcgccgg gcgcgcgccc cccaggtact tctggaccag gtccaggcgc gcgacgggca
    12661 cgaacagctt gtcgtcgccc gcgtagcgca gttcgaggaa ctccagcgtc tcgttcggcg
    12721 cgacggtcat ctccttgagg ccgacgaact cgccgatgcc gttgtccacg tgcacgacga
    12781 ggtcgccgac cttcaggtcg cgcaggtcgg acagaaacgc cctggcggcg ccccgcgtcg
    12841 tgcggtcccg ggtgcggcgc tcttcctcga acaggtcggt ctcggcgtag atgcggaacc
    12901 cggcgccggg cagccggaac ccgcgcgaga gcgcgccgac ggtgatgagc acgctcgccg
    12961 cgaaggcatc gtcgccgcga tcgatctgcg cgccgcgaag ctcgtagtcg gcgagccatt
    13021 ccacgaggcg ctcggcgcgc ccgtgggtgg ccgcggcgaa cacgacggtg tcgccggccg
    13081 cgcgcgcggc cctgacctcg gcaatccagt ccggcaggcg gttggcgaag gagagagcgg
    13141 gctgggagca ggcaggggcc ggcgaccgtg ggccgggcgc ttgcggcgat ccgcctgcgc
    13201 tgtccgcgtc gtgaggatgc ggcccggggg cgtcggacgc cggggcttcc agcccgagct
    13261 cttcgaggac atcggcgccg gcgaggtgcc gctcgacgtc gggccagccg acgagaatcg
    13321 actcgggcgc gggaaggccg ctccgccccg cagcctcctc cggcgcctgc gcccgcgcaa
    13381 acccggctgg cgcgcggctc gcggcctcgc gcgcggcggc gtagctcgcc gcgatctgcg
    13441 cgaggtgctg ctcgatccgc gcgcgaatct cgtcgggctc gagcgcgaag aggacgggcc
    13501 gcctgggccc ggccaggtag tcgaacacag gcacgcgcgg ccagccgccg ccggcgacgt
    13561 ccccgagcgg cacgatctgc agccgatcga tcgtttccac cgatcgctgc gtctccggcg
    13621 cgtagcggcg gatggtctcg atggtgtcgc caacgagttc gatgcgcgcg ggcaggtcgt
    13681 cgccggccgg aaacacgtcc acgacgccgc cgcggatgca gaactcgccg tggtcatcga
    13741 cggggtcccg gcgcgtgaag cccgcgtcca cgagcgtgtc cgccaggcgc ccggtgtcga
    13801 tctcgccgcc cggcacgaga tcgagggcga gcgcgacaat cgcgtccggg gggctgacgc
    13861 gcgggaggag ggcggccgcc gaggcgacca cgacgcgcgc gagcccggtg gccgcggcat
    13921 ggagcgccct ggcgcgggcg gacgcaatcc gaaggtgggg cgcgaggcct cgatacggat
    13981 cgacttcagg cgacgggaac ggcagcaccg tgcgctcgag ttcggccgcc gaggcgccct
    14041 cgagcccgcc aaggaagaag cgcacgtcgc cgacgatctg ctcgacgtcg cggtcggacg
    14101 cgacgacggc cagcaccagc tggcgccgcg gatcgtccga ggccgcgccg gcgatccaga
    14161 aggccttagc ggcgggagtc aggccggcga cgcgcacacg cccgcgtttc gcctcgcgga
    14221 cgaaacccgc gcgcgcacgc gcggcccgca gcagctcctg cagcctcagc gacggcaacg
    14281 ctcttccagt ctatcccgga cgccgcggac gcgacggcgg cccgccgccg tcccgcgcct
    14341 gcgccggggc gcggcgagcc tcacctcctc gccaagagga cgcggccgtc gttcaggatc
    14401 gcgaggtcct gcaggcgcga cacgcggctc agctcttcga gcggccacag cgactccgtc
    14461 tgcctgcggc tgaaccagac gaggactgcc cggccctccg gcggccacag gccggcctgg
    14521 agagcggggc caggcccctt ctccggcgac caccgggcgc gcaggccggt gagaaggtag
    14581 acggcgtccg gggcgttgct gtagacaggc gccgcccgca ggtcgtcaag atgggcgagg
    14641 aggtagccgg ccgtagcgct gtcctgccac ccgcgctccg cgatgcccac gccggtttcg
    14701 ctgaaccagc tggcggcgcg gacggttcgc acgaccggcc acgcgagcat gcctgccacg
    14761 agcgcggcga cgacggactt cctgaggacg acgatcctcc ccgcgggcct cgcgcgcagc
    14821 agcaggtcca gcaggcatgc ccccagcacg gcgacgggga tcgcgatcgg cgcgagggcg
    14881 cggtcgctga tctgcgcgcg cgcccccgcc tcggtcgtcg ccacgacggc cagcgcgaag
    14941 atcagcgcca gtgccgccgt tacccagatg cgcgcgcccg ccacggtccc cgcaggcgga
    15001 gcgtcatgcc caccgggcct gccgctgccc cggatcacga cgaacaccgc ggcggcgacg
    15061 aggagcatca cggcaagcgg cccggcgatt ccgacgcggc cgacgggcgc ataccacgcc
    15121 acgacgccgg tggcggcggc ctcgaggttc cgcgcgacgt ccacggcact cgcgccgggg
    15181 cgcgactgga ggagcgccgc ggacaccccc ggccgggccg ccagccatgc cgccagcggc
    15241 acgcttgccg ccgccgcgaa gagggcggcc cgtccaattc gcgcgagcac gcggccgggg
    15301 ccgtacgcca ggacgacgat cgccgcccag gcgatcacgg ccgcgccgct gtatcttgcg
    15361 aggctcgcgg cggcggcgaa cgcggcgagg gccgccaggg cccctcgtgt tcgcctggcg
    15421 gcgtacgcgt ccccggcgaa gagcgcggcg ccaacgagcg cggccaggag gaggtccggc
    15481 catgtcatgg ccgacacgta gaacaaggga taggccgcgg cggcggcggc ggcggcgaac
    15541 atcgccgggc ctggcgccag atgcagccgc cgcgacgcga tggcgcccgc gacggccacg
    15601 gccgcgaggt agagcagcac gctcacgggg cccgcggctc gcaggggatc gctggccgtc
    15661 aggaggccgg cacaggccag gaccgccggg tacagcggag gctgcgcgag gagcggcccg
    15721 ccgtcgtacc acagcaagcc gcggagcccg gccaggctcc gggcggccga gacgtagccg
    15781 gcggattccg gcgacagtcc cgggccgtgc ccggcgttcg cccacagggc gagcgccgcg
    15841 cctgcgaggg cgacgagcgc gctcctccgg tctgccgtcg acagcaccta cgccttcagc
    15901 ttcgcgatga cggccgtggt ggaatggcct tgctcgacgg gcacgcggat gacgacgccg
    15961 ccgcgcgcct cgacgatgtc gcggccgacg atccgatcgg cgggccagtc ggcgcccttg
    16021 acgagcacgt cgggctggat cgcggcgatg atgtcgtgcg gcgtgtcctc gtcgaagatc
    16081 accgcgaggt ccacgacgcc gagcgcggcg accacctcgg cccgttccgc ctcggggtgg
    16141 acggggcgcg tcggcccctt gttggcgcgg acggatcggt cggagttgac ggcgacgacg
    16201 agcgcgtcgc cgctctcgcg ggcccgggtc aggtagcgca cgtggcccgg gtgcaggatg
    16261 tcgaagacgc cgttcgtgaa cacgacccgc ttcccctcgc gccccagcgc ctcccgccag
    16321 gcgacggcct cctctcggct tgcgaccctg cgcgtcacga tcccgccacc cgttcgctga
    16381 cgcgcacgcc gccgctgaag ccccacgagt ggcgcggccg gtagatgccc gcgtcccgcg
    16441 tccgcgactt cacccggaag ctgtacagca ggcggtggta gtcgtaggga tatccgtcga
    16501 gcttgtgcag cgccacgtcg agcttgtacg tcccggccac caggtccagg gcgtcgatcg
    16561 tgacgacggc ctccccttcg ccctcgaggc gttccccctg caggtcctcg atgttcgtgt
    16621 tcgtcccgaa ggcgcacacg ccgtcggcgt tgaagatgcc gatcccgatc acgaagtcgt
    16681 cgagcggcgc cgcggccttc agggagaggc ggatcgcgac gcgctcgccg gaatagaaga
    16741 cgcgggcggg ctggccgtct tcgccgacga ggcgcacgtc ggtgatctcg ccttcgcgcg
    16801 atccccaccg gccctcggcg gcctggaaca tgtcgggcgg cgccgcgcgc ggggattcgg
    16861 ggcgagggat tcgggactcg ggttcggccg gagcggcgtc gtgccgttcc accgcctccc
    16921 tggccttggc gtcggcctcg gccatgtggc gctcttcctg cttctcgacg tccgtgatgt
    16981 aggccgacac cacccgccgc gggtcgccga cggcccgggc gcgcccgtcg tccagccaca
    17041 gcgcttcgtc gcagaaccgc tcgaccaggt tcagcgcgtg ggtcaccagc agcacggtct
    17101 tgttgcgccg gcggtactcg gcgaacttgt cgaggcactt gtgcgtgaac gcctcgtcgc
    17161 cgacggcgag cacctcgtcg atcaggagga cgtcgggatc gacgttgatg gccacggcga
    17221 acccgaggcg catgtacatg ccggaggagt acgtcttgac cggcgcgtcg atgaagtcct
    17281 ccagctcggc gaactccacg atctcgtcga agcggcgagt gatctcgcgc ttcgtcaggc
    17341 cgagcatgat gccgttgatg aagacgttct cgcgccccga gatctcgggg tggaacccgg
    17401 cgccgagctc gatcagcgcg gagatccgcc cgcgcacgga cacggtgccc gtcgtcggct
    17461 tcgtgatgcc ggcgaccagt ttcagcgcgg tgctcttgcc cgatccgttg cgcccgacga
    17521 tgccgaaggt ggagcccgcg cggacgccga aggacacgcc gcgcagcgcg tggaacgagt
    17581 cctcgggcct caggtcgccg acgagggtcc cggacaggat cgcgctcttc agcgtggcaa
    17641 actgcttccg ccgcgcgtac ctgcggtagg tcttcgtgac gttgacgacg tcgatggcgc
    17701 gcgcgggcgc tggcatcata cttcctccgc gaacgtgtcg cggagccggt cgaacaggta
    17761 gtagcccgcc agaaagagcg cgaccgaccc cgcgccgagc gccaggagcc acttccagtg
    17821 gccgaagtcc ccctggtaga acaggatctc ctggtacgag atcgccaggt gcgtgaacgg
    17881 gttgccgttc aggaaccgct tggcgagggc cggcgcatcc tgcatcgagt agatgatcgg
    17941 cgtcgcgaag aaccagaacg tgacgaggtt cgacaggatg tccttcaggt cgcggaagtg
    18001 cacggtcagc gcggacacga tgagggcgag gccgagcgtg aggacgagct gcacgagcac
    18061 gacgacgggg aaccaggcga gctccgacgc ctgcagcggc gcgcggtagt agatcaggaa
    18121 gcccacgagg atcggcaggc ccaggaagaa atgcaccatg ttggccagca cggtgacgaa
    18181 gggcagcacc tcggccggga acatcacctt cttgaggagg ttgcctcccg agatgagcac
    18241 gttggccgac tcgagaatcg acgaggagaa ccaggtccac ggcaggatgc cgcaaaacag
    18301 gaacagcgcg tacgggtcca ggttcggctg ccgcgcgggc aggacgtagc tgaagacgaa
    18361 cgtgtacacg agcagcagga gcagcgggtt gatgaacgac cagaagaagc ccagcaccga
    18421 gccgcggtag cgcgccttca gctcgcgcgc aaccatggac tggatgagct ggcggtactt
    18481 caagaggccg cggaggttct tcagcatgcc acctcgacga cgacgacggt gatgttgtca
    18541 gggccgccgg cggcgatcgc cgcctccacc agggcgcggc aggcctcctc cggccgtccc
    18601 ggcggcgtgc gcgcgagaat ttcggcgacg gcctcgttcg tcagcggcga cgtcaggccg
    18661 tcgctgcaga ggatcgcgcg gtcgccgggc tctagcgaca cgggcgtcag ctcgacgcgc
    18721 ggctcctccg ccccggcgag cgcgcgcgtg acgaggttcc gcagcgggtg caccctggcc
    18781 tcgtcctcgc cgaggacgcc cagcgcgact tgctcctcga cccaggaatg atcccgcgtg
    18841 atctggtcga gagccccgcc gcgccagaga taggcgcgcg aatcgccgac gtgggcgacg
    18901 agggcgggaa tactcggggc taggggctgg gcgctagggg ctagtcggct tggaggctgc
    18961 ccaccagcgc ctagcgccaa gtccctagtc cccaagagca ggcacaccgc ggtcgtcgcc
    19021 atgccgcgca gggcgggttc gtcctccatg cgcagccgga tctcgcggtt tgccaggagc
    19081 agcccgcacg ccaggcggtt gccgtcgagg ccccgctcgg gccggtactc gaacggccag
    19141 gcgaagtccg ccgtgacgcc gtcggtgtgc tggatgaagt cctcgagggc cccggccgcg
    19201 acccgggacg cgacctcgcc ggccgcgtag ccgcccatgc cgtcgaagac cgcgaacaag
    19261 cccagcccgg gccgcgcgac aagggcgtct tcgttgagcg gccggcgcag cccggggtcc
    19321 gtcagcgatg ccgacagcac ctgcacatca gcctcgcagc ttggccttcg ccgccacctg
    19381 gatccggacg atcgacttca gcagggagat cctcgcgcgc tcgagatcga tgtcggcgct
    19441 gccgcttcgg gcggcctgca ccgccgtctc ggccctgcgg cggcgctcct cggcccgggc
    19501 gacgtcgatc tcctcggcgc gctcggcgac ctgcgccagc agccgcaccc ggtccggctg
    19561 gacctcggcg aagccgaacg cgatggcgag gtagcgcttc tcggcgccct ggcggtacca
    19621 cagctgcccc acccgcaggc tcgtcaggag cggggtgtgg cccggcagga tgcccaggta
    19681 gccggcgacg ccaggcagct ggacctcgtc cacctcctcg cgcaccacgg cgtggtcggg
    19741 cgtgacgact tccaggacga ggcggggtgg caggggcatc
//
LOCUS       DAOSIF010000008        17987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_3343_length_17987_cov_5.010261, whole genome shotgun sequence.
ACCESSION   DAOSIF010000008 DAOSIF010000000
VERSION     DAOSIF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17987
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3343_length_17987_cov_5.010261"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLT35_00730"
     CDS             <1..656
                     /locus_tag="PLT35_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG27776.1"
                     /translation="LAVDHEGVAPARWLNGLGVSAFILKYRLKEYGHPAPLRDVLRAV
                     RTVRANAASFDVDPARIGVMGFSAGGHLAASSGTLFDAPEGRTGAALDRVSARPDFLI
                     LVYPVIQLNTPFTHAGSRQALVGASPAPGLVDALSLDTRVTKDTTPAFLVHGGTDTGV
                     PPENSLAFYAALRRAGVPAELHIYEKGAHGFGLRPGLGPVSDWPRRCAEWLAARGLI"
     gene            756..2747
                     /locus_tag="PLT35_00735"
     CDS             756..2747
                     /locus_tag="PLT35_00735"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOG27777.1"
                     /translation="MRTYAGFVTLVAACSISLAAQPAAAQRARKMPAGLLTVASPSGA
                     IVATVGTTGGVTWSVSMKGRQIVKPSPIAMTLDNGRVLGGAPVAVGTTTRSADRVLKP
                     VVRVKRAEIRDHFNERRVDFAGGYALLVRAYDDGVAYRWATTLPGDVTVIGEDATITF
                     ADDYPLWFPEETSFQSHQERQYKRLSISELQAGQFSSLPAMADLGGGAKAVITEADLL
                     DYPGMDVMAGPEPGSLKGLFPAYPKQVEAANDRDEKVAEREPYIAKTRGTREYPWRVL
                     VLAERDADLLGTDIVYRLASETTMDDTSWIAPGKVAWDWWNDLNITGVPFRAGVNTDT
                     YKHYIDFAAENGLQYIILDEGWYRLGDLLSVVPGLDMEAVAAHAKQKNVGLIMWVIWK
                     TLDRQMQPALDQFARWGVKGIKVDFMQREDQWMVNYYERVAREAAARRLLVDFHGSYK
                     PTGLYRTWPNVITSEGVLGLEQSKWGDMASPDHAVTFPFMRMLAGPVDYTPGAMVNST
                     KRDFRPVYNRPGSQGTRCQQLAMYVAFESPLQMLADSPSHYRREPESLAFLSAVPTVW
                     DETKPLDAKVGEYLLVARRSGREWYVGALTNWSARDLEVDLSFLGEGRFEAEIFRDGV
                     NADRNALDYARERQGVTAQSRLQVHLAPGGGFAARIVPR"
     gene            2943..3425
                     /locus_tag="PLT35_00740"
     CDS             2943..3425
                     /locus_tag="PLT35_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOG27778.1"
                     /translation="MAIPASIERYVTSHGMSFTPIHYHVAFTAQEEAAAAHVRGREWA
                     KTVVCLADGKPVLALLPAHYRVDVNALKALTNATQVRLATEHELAGLYPDCDTGAMAP
                     FGELYGQDVYMDDSLAADAEIVFHAGTHADAMRMRLDDFLALVRPVVGRFAGPAHGGH
                     "
     gene            3455..4411
                     /locus_tag="PLT35_00745"
     CDS             3455..4411
                     /locus_tag="PLT35_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG27779.1"
                     /translation="MNANRRRFLTTTSMALAAAAAGRSALFARVSQSAGAGQAGPPAA
                     PPTAFAALRGTIGTFVGQGGTIGYHIGKDGVVVVDAQMAPTAQIALQGIRERAGGRMI
                     DVLVNTHHHGDHTEGNAVFRPAVKRILAHVNVPSLQRAAAAQQAAARPGAPPPEPVVA
                     DATFVNTWEEDVGGERLALKYYGAAHTSGDAVVTFAKTDVVHMGDLVFNRRHPYIDRP
                     AGASISNWIKVLEAVAGEHGSDTQYIFGHAGERFPVTGARADLLYQRDYLTALLEHVR
                     RELTAGKPKDEIVKTAAALRGFPDHGPLVPRVLTAAYEELAG"
     gene            complement(4632..7376)
                     /locus_tag="PLT35_00750"
     CDS             complement(4632..7376)
                     /locus_tag="PLT35_00750"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27780.1"
                     /translation="MRTLHPAARVLRALALAALAAAIPADLAAQALPPDVAKAFSFRA
                     IGPTRQSGRFVDFAVPAREPHTIYAATGSGGLWKSVNNGISWVSIFDTQPVVSIGDIA
                     VADTDPSIVWVGTGEHTSSRSTYWGDGVYKSTDGGRTWTNMGLADSHHIGRIVIHPKD
                     PNIVYVAALGHLYSPNDERGVYKTTDGGRTWTKSLEVKADGRMVGAADLVMDPKNPKV
                     LYAAAYDKERLPWTFNLGGPGSGIYKTTDAGRTWTKLGGGLPGGMLGRIGLDVYAKNP
                     QIVYANIENANKPGVPDADRLAELRAGKSSAGMIGEEVYRSDDGGKTWRKASPDKQRI
                     GGNPGYYYMQIRVDPNDANHVYVLSVGMHHTTDGGRTWSVPFRFGGDNHAMWIDPANS
                     KHILLGYDHGMGITYDGGANWYHPDELPLAQFYAVGFDMMQPYNVAGGMQDNGSVRGP
                     STKRGGGRIGFEDWQSVGGGDGFYNETDPVTNRYLYNESQFGSLSRTDLYTGEVKNIT
                     HRDPALRFNWNAPILISPHDSNVLYHAANKLLKSAYRGEGWVEISPDLTTNDPTKLPT
                     GKGGDGNVTYCTITSVDESPIVRDLLWVGTDDGNVQMSKDGGKSWTKLNDRIPGHPGY
                     WVSRVVASSHAPGTAYVTFTGLRNDDFRAFVYKTTDYGETWASIAGNLPAKSVNVIRE
                     DPYNPNLLFVGVDFGLYVTIDGGRIWQEMRNGLPTQPVHDLKIHPRERDLIVATHGRG
                     IFIADISALEEVSASMLAKDFHLFDVEPKVRWTVRRPAMSAFTNFDGESEPNGVMIRY
                     YQKAPAAGGVSVAILQGTRVIAETKGPNAAGVNQLLWNMRAAPQTIPGQPAPPAAGRG
                     AGSARPAGAGAQAEPAYASFGGAVPVPVGEYAVVVSAGGAAFTKKIQILEDVWFDRVF
                     "
     gene            7673..8563
                     /locus_tag="PLT35_00755"
     CDS             7673..8563
                     /locus_tag="PLT35_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG27781.1"
                     /translation="MTTRAAAAGPALDARLVRRLYDRSGAERWGLAEQDFAAALARSV
                     GRRFAGSAPSASEAAQYLESLRLEDLALACACARGRESAWERFVQEYRPILLRIASRG
                     QQTDRGRDVADSIYAELYGLEVRDGARRSLFDYYHGRSTLAGWLQAVVAQRLVDLARA
                     ARRFEPLSDDERAAEGAAEDGARDAELEPDRRRLLGLARQAFETAVAGLESRDRLRLA
                     LYYTQDMTLAAIGRVLGESEATASRKLERARAALRAAIQRHLREVGRLSDREVAQCFE
                     HARTDPAFDLARTLPPPGST"
     gene            8722..9858
                     /locus_tag="PLT35_00760"
     CDS             8722..9858
                     /locus_tag="PLT35_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOG27782.1"
                     /translation="MSTSPSRDTVDADVERLLRAALKPSAGGRAGECPDAESLAAFVE
                     GRLPPRERAPLDAHFAACGRCQETLAVMGAAPPLPLVAPVRPGWFARITAPRLRWLVP
                     VSAAAAVAAVFFATRPVIAPGEHAATAEAPVQMAHAAPPELGPAGAARNEVPEAALQR
                     DASARTGAAADELKPGGPRRPAAVLADEGAAGEGGRQAVSLGMPTAEQAVPAAAAMRE
                     TRDVREVAVSEGAKPAGLAKSATQSEAAGAPLAASVAFRERDAAGALLPVVAAPDGVV
                     QWKLGGEGRVWRSEDGGVRWREQRTGVGAVLTAGSAISADVCWIVGEGGTVLLTVDGA
                     FWQQRPFPFLADLVDVRAASARSAVVTARDGRRFETRDGGLTWR"
     gene            9944..11398
                     /locus_tag="PLT35_00765"
     CDS             9944..11398
                     /locus_tag="PLT35_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27783.1"
                     /translation="MMDTQLRTAVLAAAAILTSALASGPTITALASQDSAAAGGQSDL
                     AVTVYNSSLALVRDVREFALPSGESDLRFVDIAATVNPATVHFRSLTDPARVAVLEQN
                     YEYDLLEPEKLLRKYVGREVTLVRTGVENGTTKREEVKATLVSLNNGPIWKIGNEYVT
                     GLGSDHLRFPELPSNLYSRPTLVWKLLNRGGPRHRIEASYLAGGLSWQSDYVLTVGRD
                     DASAGLDGWVTLTNESGTAFRDATLQFVAGDLNRVQPAARKQMLEMMADARGAAPAMA
                     QEAFAEYHLYTLGRRSTIENQQTKQLALLSGSGVPVEKRYVVDGQAFYYRNRQSPGRP
                     LKDKVQVYYRFRNDAQSGLGAPMPAGILRVYQADAKGGLQFVGEDRIDHTPKDETIDV
                     KIGSAFDVVCERNQTDFEKISELVYEMEYAVTLRNHKTTPVTVRVNEPIGGTWKMLRS
                     SHAWEKTAAWAAQFTVPVAADGTAVLAYRVRVTY"
     gene            11566..12954
                     /locus_tag="PLT35_00770"
     CDS             11566..12954
                     /locus_tag="PLT35_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOG27784.1"
                     /translation="MFDAATYQSRRRRLSKDVGSGLILLLGNDEVGMNYAANAYHFRQ
                     DSTFLYFFAVNLPGLAGLIDVDAGTETLFGDDLTVADIVWTGPQPTIAKLCKPAGIAS
                     SAPMAELETRLAAAIAKGRRVHFLKPYRAEHTLKITALLGLKPTIVASYRSEALHAAV
                     VAQRNVKSADEVAEIERAVGVSREMYLAAMRAARPGRREYEVVAEIVRVQKANGCELA
                     FPIICSVHGETLHNHAHANLMKAGDVMVLDSGVETERGYASDITRTIPVGGRFTAEQR
                     VIYDLVLKTQLAAIAAIKPGVRYKDVHLLASRSFASGLKEIGLMKGDPAEAVAAGAHA
                     LFFPHGLGHMLGLDVHDLENIGEQHVGYEPGLERSGQFGLSYLRLARTLKPGFVLTVE
                     PGLYFIPQLIDQWKADKRHAAFVNYREVEKFRNARGYRIEDDVLVTRSGNRVLGPPIP
                     KKAEDVEAACRA"
     gene            13119..15482
                     /locus_tag="PLT35_00775"
     CDS             13119..15482
                     /locus_tag="PLT35_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOG27785.1"
                     /translation="MALSYTQWLHTQWPAGTIEPRPETDRDGRTSVRGVRVAGELAGH
                     VLLREAADSGARAVRAIAAERGFKPSADPSVPDLAVIGAGAAGIAAAIEARRAGLSVV
                     VYEAAAPFASIAGLARGRSIVTPTAAAPAPAPGDGASAGALAITAGRKEDVLADLERQ
                     RREAGIETEASLVERIESRKGAVALHHAGGRVTLAQRAIVAIGRSGVFPPLGCPGDDL
                     DKVRTRILDPTEHAGKRALVVGDGDHAIETAVALATCGAKVTLACRGAEFDRPRAENL
                     VKLRRITEQPEAQVHIESPSAERVTTATMRNMKSAEGPGSLRVLMATTVERVGPAAVT
                     LVGADGAEQVVPNDVVFSMIGRAEPEGLLRRSRLRLRGTRDAAVWLGLAVSICVFVLV
                     LHWAVSRPELPIQRAVAAYRAFPYDLPGLVDSAGGALADLAARETHLLYTLKSSLGTP
                     LFHLSLAWCLAVTIFGARRIARRRTPYVFWQTLTLIAVQWIFLIVIPHLALPWAGRNG
                     YFEPRQPLRWMADQLFERGDGFLGHERAYWRAYGFVLPFPLNVFNVFTDRPMWLWMAI
                     ALLQALVGVPLMVRRWGKGGFCGWVCPAGALAETVGDVHREKMPHGPLANRVNMIGQG
                     VLALVLALLALRFAAWSLGPRSWAQTVFREAFSGLPFFNYTWLVVLFMAGAVGIGATF
                     FFSGRVWCRFACPLGALSHIHARSSRFRILPEKAKCISCGVCTSVCHAGVDVMNFANK
                     DQPVRDPQCVRCSACLEQCPTGALAFGLVRKSGEVVFDRLRAGRGQA"
     gene            complement(15557..17611)
                     /locus_tag="PLT35_00780"
     CDS             complement(15557..17611)
                     /locus_tag="PLT35_00780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG27786.1"
                     /translation="MPRLTRTVALAAAAAVLVAVTLAAGPAAQAPAVAARRAITLDDL
                     AKIKSVGDPQRSPDGKWVAYVVTSADLEKDKRDSDIWMVSWDGAQTVRLTSGPEKESA
                     PRWSPDGRYLSFLSSRGTEEEKKKGAQIWLLDRLGGEAQKLTEIEGGVDEYSWSPDSK
                     RFVFTKSDQDPADEPEKMEGWKRKTLPPIVLDRYRFKRDRDGYLKRLYSHVYVFDIAA
                     KKAEQVTSGPFDDSEPSWSPDGTKIAFVSTRTGPDPDRHENSDIYVVDAKPGAEPRQV
                     TTSTAADAGRPAWSPDGRWIAYFQGDETRYLAYEINKLAIVPAAGGAAKVLTAALDRP
                     VGGPISWSPDSRMLTFTVEDDRASYVGRTSVAGGQVEKLTAGRRTVSDVSLGADGRLT
                     LLAGTSTATPEVHALENGTLRQLTKVNDELFAQLQLATTEDFTSTSKDGTVVNGLIVK
                     PASYAAGTKYPTLLIIHGGPNGQDDYSFTFENELYAANGYVVLNINYRGSSGRGEAFQ
                     KAIYADWGNKEVVDLLGAVDQAVASGVADPERLGIGGWSYGGLLTDYTIASDTRFKAA
                     VSGAGSALQSSMYGVDHYIVQWDTEIGQPWKAQDLYLKVSYPFWHADRIKTPTLFLCG
                     EKDFNVPLAGSEQMYQALRSLGVDTELVIYPEQFHGLTKPSYVRDRYERYLAWFDKYL
                     KR"
BASE COUNT         2618 a         6221 c         6418 g         2730 t
ORIGIN      
        1 gattggccgt cgaccacgag ggggtggcgc ctgccaggtg gctgaacggc ctcggcgtgt
       61 ccgcgttcat cctgaagtac cggctgaagg aatacggcca cccggcgccg ctgcgcgacg
      121 tcctgcgcgc ggtacggacc gtgcgcgcga acgcggcgtc cttcgacgtc gacccggcca
      181 ggatcggcgt gatgggtttc tccgccggcg gccatctggc cgcctcgtcc ggcacgctgt
      241 tcgacgcgcc agagggaagg acgggcgccg cgctggaccg cgtcagcgcc cggccggact
      301 tcctcatcct cgtctacccg gtgatccagc tgaacacgcc gttcacccac gccggatcgc
      361 gacaggcgct cgtgggagcg tcgccggccc cgggccttgt cgacgcgctg tcgctcgaca
      421 cccgcgtcac gaaagacacg acgccggcgt tcctcgtgca tggcggcacc gacaccggcg
      481 tgccccccga gaacagcctg gcgttctacg ccgcgctgcg gcgagccggc gtgccggcgg
      541 aactgcacat ctacgagaag ggtgcgcacg gcttcggatt gaggcccggc ttgggccccg
      601 tgtcggactg gccgagacgg tgcgccgagt ggctggccgc ccgcggcctg atctgataag
      661 gggtcgggac gattttcgca accgacaaaa ttgaattaca taatccgtac gttgcgaaaa
      721 tcgtcccgac cccttcggca gagagagagg aacacatgcg gacatacgca gggtttgtga
      781 cgctcgtggc ggcgtgctcg atctcgctgg cggcccagcc cgcggcggcc cagcgcgcgc
      841 ggaagatgcc ggcagggctg ctgaccgtcg cctcgccgag cggggcgatc gtcgcgacgg
      901 tgggcacgac cggaggcgtc acgtggtcgg tgtccatgaa ggggcgccag atcgtgaagc
      961 cctcgccgat cgccatgacg ctcgacaacg gccgcgttct cggcggcgcg ccagttgccg
     1021 tcggaacgac gacgcgctcg gcggaccggg tgctgaagcc cgtcgtgcgc gtcaagcgcg
     1081 ccgaaatccg cgaccacttc aacgaacggc gcgtggactt cgccggcggc tacgcgctgc
     1141 tcgtgcgcgc gtacgacgac ggcgtcgcct accgatgggc gacgacgctg ccgggcgatg
     1201 tcacggtcat cggggaggac gcgacgatca cgttcgcgga cgactacccg ctctggttcc
     1261 cggaggagac gagcttccag tcccaccagg agcggcagta caagcggctg tctatcagcg
     1321 aactgcaggc gggccagttc tcctcgctgc cggccatggc cgacctcggc ggcggcgcca
     1381 aggcggtgat cacggaagcg gacctcctcg actaccccgg catggatgtc atggcgggtc
     1441 cggagcccgg ctcgctgaaa gggctgttcc cggcgtaccc gaagcaggtc gaggcggcga
     1501 acgatcgcga cgagaaggtg gcggagcgcg agccgtacat cgcgaagacc cgaggcaccc
     1561 gggagtaccc gtggcgcgtt ctcgtcctgg ccgagcgcga cgcggacctt ctcggcaccg
     1621 acatcgtcta tcggctcgcc tcggagacca cgatggacga cacgtcgtgg atcgcgccgg
     1681 gcaaggtggc gtgggactgg tggaacgatc tcaacatcac cggcgtgccc ttcagggctg
     1741 gcgtgaacac ggacacgtac aagcactaca tcgacttcgc cgccgagaac gggctgcagt
     1801 acatcatcct ggacgaaggc tggtacaggc tcggcgacct cctgtccgtg gtgccgggtc
     1861 tcgacatgga ggcggtggcg gcgcacgcga agcagaagaa cgtcggcctg atcatgtggg
     1921 tcatctggaa gacgctcgac aggcagatgc agccggcgct cgaccagttc gcgcggtggg
     1981 gcgtgaaggg catcaaggtg gacttcatgc agcgcgagga tcagtggatg gtcaactact
     2041 acgagcgcgt ggcgcgcgag gcggcggcgc gcaggctcct ggtcgacttc cacggctcct
     2101 acaagccgac cggcctgtac cggacctggc ccaacgtgat cacgagcgag ggcgtgctgg
     2161 gcctcgagca gagcaagtgg ggcgacatgg cgtcgcccga ccacgccgtc acgttcccgt
     2221 tcatgcgcat gctggccggc cccgtggact acacgccggg cgcgatggtc aactcgacga
     2281 agcgcgactt caggcccgtt tacaaccggc cggggagcca gggcacgcgc tgccagcagc
     2341 tggcgatgta cgtcgccttc gagagcccgc tgcagatgct ggccgactcg ccgtcccact
     2401 accgccgcga gcccgagagc ctggcgttcc tgtcggccgt gccgacggta tgggacgaga
     2461 cgaagcctct cgacgcgaag gtcggcgagt acctcctcgt ggcgcgccgg agcggacgcg
     2521 agtggtacgt cggcgccctg acgaactgga gcgcgcgcga cctcgaggtc gacctgtcgt
     2581 tcctcggcga ggggcgcttc gaggcggaga tcttccgcga cggcgtgaac gccgaccgca
     2641 acgcgctgga ctacgcgcgc gagcggcagg gcgtgacggc gcagagccgg ctgcaggtcc
     2701 atcttgcgcc cggcggcggg ttcgcggcgc gcatcgtgcc gcggtaagca gaaccgccgc
     2761 tcgcccgaca ggcgggccgc cgccggccgg ccgcctgggc gcatcgacgt gcgcgcgctt
     2821 gacgccggcc cgtccggggc cgagactacc gagtaccgat cgtcgaactg tgctccgggc
     2881 cgccgccccg ccccgcggga cggccggggt gccggctcgg gccctcgccc gggaggagag
     2941 ccatggcgat tccggcgtcg atcgagcggt acgtaacctc gcacggcatg tccttcacgc
     3001 cgatccacta ccacgtcgcg ttcaccgccc aggaagaggc ggccgcggcc cacgtccggg
     3061 ggcgcgagtg ggcgaagacg gtggtatgcc tcgccgacgg caagcccgtc ctggcgctgc
     3121 tgccggcgca ctaccgggtg gacgtgaacg cgctgaaagc gctgacgaac gccacgcagg
     3181 tccgcctggc caccgagcac gagctggcgg ggctctatcc cgactgcgac acgggcgcga
     3241 tggcgccgtt cggcgagctc tacggccagg acgtctacat ggacgacagc cttgcggccg
     3301 acgcggagat cgtcttccac gccgggacgc acgccgacgc gatgcggatg cggctcgacg
     3361 atttcctggc gctcgtccgc ccggtcgtcg gccgcttcgc cggccccgcc catggcggac
     3421 actgaccatc cagcgagaga aggagcatgc gcacatgaac gcgaaccgcc gccggtttct
     3481 cacgacgacc tcgatggccc tggccgccgc ggcggccggc cgctcggcgc tcttcgcgcg
     3541 cgtttcgcaa agcgccggcg cggggcaggc cgggccgcca gccgccccgc ccacggcgtt
     3601 cgccgcgctc cgcggcacga tcggcacctt cgtggggcag ggcggcacga tcgggtacca
     3661 catcgggaag gacggcgtcg tggtcgtgga cgcgcagatg gcgccgacgg cgcagatcgc
     3721 cctgcagggc atccgggagc gcgcgggcgg ccggatgatc gacgtcctcg tcaacaccca
     3781 ccaccatggc gaccacaccg agggcaacgc cgtgtttcgg ccggcggtga agaggatcct
     3841 ggcccacgtg aacgtgccca gcctgcagcg ggccgcggcg gcgcagcagg ctgccgcgcg
     3901 tccgggcgcg cccccgccgg agcccgtcgt ggcggacgcg acgttcgtga acacgtggga
     3961 agaggacgtg ggaggggaac ggctggccct caagtattac ggggcggcgc acacgagcgg
     4021 cgacgccgtc gtcacgttcg cgaagacaga cgtcgtccac atgggcgacc tcgtgttcaa
     4081 ccgccggcat ccgtacatcg acaggcctgc gggcgcgtcc atctcgaact ggatcaaggt
     4141 gctcgaggct gtggccgggg agcacggcag cgacacgcag tacatcttcg ggcatgccgg
     4201 ggagcggttc cccgtcacgg gtgccagggc cgatctgctc taccagcgag actacctgac
     4261 ggcgctcctc gagcacgtgc gacgagagct gacggcgggc aagccgaagg acgagatcgt
     4321 gaagacggcc gccgcgctcc gcggattccc cgatcacggt ccgctcgtcc cgcgcgtgct
     4381 gacggcggcg tacgaggaac tggcgggtta aggcgcgggg acagaccgcg ctgggattca
     4441 atccgcgctt ccgggcggcg gcccggagca gatggaccct gctggcgcgc ccagcctccg
     4501 cgacgtgccg cgggcgcagg cttcggcgcg ccaggccatg ccccgggttc agactcccga
     4561 tgcccgggtt ccgactcccg attcctgatt ctcgattacc ggcctccgag ccccaagccc
     4621 ggcggccctg gttagaacac cctgtcgaac cagacgtcct ccaggatctg gatcttcttc
     4681 gtgaaggcgg cgcctccggc cgacacgacc accgcgtact ccccgacggg gaccgggacc
     4741 gcgccgccga agctcgcgta cgcgggctcg gcctgcgcac cggcgccggc gggccttgcg
     4801 cttccggcgc cgcggccggc ggctggcggc gcaggctgac cggggatcgt ctgaggcgcc
     4861 gcgcgcatgt tccacagcag ctgattgacg cccgcggcgt tcgggccttt cgtctcggcg
     4921 atgacgcgcg tgccctgcag gatggccacc gacacgccgc cggcggcggg ggccttctgg
     4981 tagtagcgga tcatcacgcc gttcggctcg ctctcgccgt cgaagttcgt gaaggcagac
     5041 atggcgggcc ggcgaacggt ccacctcacc ttcggctcga cgtcgaacag gtggaagtcc
     5101 ttcgcgagca tcgacgcgga cacctcctcg agcgccgaga tgtcggcgat gaagatgccc
     5161 cggccgtgcg tagccacgat gagatcgcgc tcgcgcggat ggatcttcag gtcgtgcacc
     5221 ggctgggtcg gcaacccgtt cctcatctcc tgccagatcc tgccgccgtc gatcgtcacg
     5281 tacagcccga agtcgacgcc gacgaagagc aggttcggat tgtacggatc ctcgcggatg
     5341 acgttcaccg acttcgcggg caggttgccg gcgatcgagg cccacgtctc gccgtaatcc
     5401 gtcgtcttgt acacgaacgc ccggaagtcg tcgttgcgca aaccggtgaa ggtcacgtaa
     5461 gcggtgcctg gggcgtgcga cgaggcgacg acccggctga cccagtagcc ggggtggccc
     5521 gggatccgat cgttgagctt cgtccacgac ttcccgccgt ccttcgacat ctgcacgttg
     5581 ccatcgtcgg tgccgaccca gaggaggtcc cgcacgatgg gcgattcgtc cacgctcgtg
     5641 atggtgcagt acgtcacgtt gccgtcgccg cccttgcccg tgggcagctt cgtcgggtcg
     5701 ttcgtcgtga ggtccgggct gatctcgacc cagccttcgc cgcggtaggc cgacttcagc
     5761 agcttgttcg ccgcatggta gaggacgttg ctgtcgtgcg gcgagatgag gatcggggcg
     5821 ttccagttga agcggagcgc cgggtcgcga tgggtgatgt tcttgacctc gcccgtgtag
     5881 agatcggtcc gtgagagcga tccgaactgc gactcgttgt agaggtaacg gttcgtgacg
     5941 gggtcggtct cgttgtagaa gccgtcgccg ccgccgacgc tctgccagtc ctcgaagccg
     6001 atgcggccgc cgccgcgctt ggtgctgggt ccgcgcacgg agccgttatc ctgcatcccg
     6061 ccggccacgt tgtacggctg catcatgtcg aagccgacgg cgtagaactg cgcgaggggc
     6121 agctcgtccg gatggtacca attggcgccg ccgtcgtagg tgatgcccat cccgtggtcg
     6181 tagcccagga ggatgtgctt cgagttcgcc ggatcgatcc acatcgcatg gttgtcgccg
     6241 ccgaaccgga acggcacgct ccacgtcctg ccgccgtcgg tggtgtggtg catgcccacg
     6301 ctgaggacgt agacgtggtt ggcgtcgttc ggatccacgc ggatctgcat gtagtagtac
     6361 cccggattcc cgccgatccg ctgcttgtcg gggctcgcct tccgccacgt cttcccgcca
     6421 tcgtcagagc ggtagacctc ctctccgatc atcccggcgc tcgacttgcc cgcccgcagt
     6481 tccgcgagcc ggtcggcgtc cggcacgccc ggcttgttgg cgttctcgat gttcgcgtac
     6541 acgatttgcg ggttcttcgc gtagacgtcg aggccgatgc ggcccagcat cccgcctggc
     6601 agcccgcctc cgagcttggt ccacgtccgg ccggcgtcag tcgtcttgta gatgccgctg
     6661 cccgggccgc cgaggttgaa cgtccacggc aggcgctcct tgtcgtaggc ggccgcatag
     6721 aggaccttcg ggttcttcgg atccatcacg aggtccgcgg cgccgaccat ccggccgtcg
     6781 gccttcacct cgagcgactt cgtccacgtc ctgccgccgt cggtcgtctt gtagacgccc
     6841 cgttcgtcgt tcggcgagta caggtggccg agggccgcca cgtaaacgat gttcggatcc
     6901 ttcgggtgga tgacgatgcg gccgatgtgg tgggagtcgg cgagccccat gttcgtccag
     6961 gtcctgccgc cgtcagtgga cttgtagacg ccgtcgcccc agtaggtgct tctggaggag
     7021 gtgtgctcgc ccgtgccgac ccagacgatg ctggggtcgg tgtcggccac cgcgatgtcg
     7081 ccaatcgaga cgaccggctg ggtgtcgaag atcgacaccc agctgatgcc gttgttcact
     7141 gacttccaca gcccgcccga gccggtggcg gcgtagattg tgtgcggttc gcgcgccggc
     7201 acggcgaagt ccacgaagcg gccgctctgg cgcgtcgggc cgatggcgcg gaacgagaac
     7261 gccttggcga catcgggcgg gagggcctga gcggcaagat cggcggggat ggcggcggcc
     7321 agcgcggcca gtgccagggc gcggaggacc cgtgcggcag gatgaagcgt gcgcatgtgc
     7381 gtgctcctga ctgaagagac acggactttc cgaatcccgg gaacggctga tgcgttccat
     7441 ttcccgggga agtgacgctg accacatcgg atcgggcgcg ggcggcgccc ccgacacgag
     7501 ggcgcgacct gcgtagtgtc gccgctcccg atgcagcgcg ccaagcgaaa tcgtgctggt
     7561 cccggccgtg tcgatgccgc agccggcgcc gcagccggcg ccgggcgccg cggggacagc
     7621 gggccggcgc gcgccgccgg aacggcgcgg ccggtgtagg attggcctgt ccatgactac
     7681 tcgcgcggcc gctgccggac cggcccttga cgcccgccta gtgcggcgct tgtacgaccg
     7741 gtccggggct gagcgatggg gtcttgccga gcaggacttc gccgcggcgc tggctcgcag
     7801 cgtcgggcgg cgcttcgccg gctcggcgcc gtcggcctcc gaggccgcgc aatatctcga
     7861 gtcgctccgg ctggaggacc tggcgctcgc ctgcgcctgc gcgcgcgggc gcgagtccgc
     7921 ctgggagcgg ttcgtgcagg agtaccgccc gatcctgctc aggatcgcaa gccgcggcca
     7981 gcagacggat cgcgggcgcg acgtggccga ttccatctac gcggagctct acggtctgga
     8041 ggtgcgagac ggggcgcggc gctcgctctt cgactactac cacgggcgga gcacgctcgc
     8101 cggctggctg caggccgtcg tcgcgcagcg gctcgtcgac ctggcccgcg ccgcccggcg
     8161 gttcgagccg ctgtccgacg acgagcgcgc cgcggaaggc gccgcggagg acggcgcccg
     8221 ggacgcggaa ctggagccgg accggcggcg cctgcttggc ctcgcgcgcc aggctttcga
     8281 gacggcggtc gccggcctcg agagccgcga ccggctgcgc ctggcgctct actacacgca
     8341 ggacatgacg ctggccgcga tcggccgcgt gctcggcgaa tccgaggcca ccgcgtcgcg
     8401 caaactcgag cgggcgcgcg cggcgcttcg cgcggcgatc cagcggcact tgcgcgaggt
     8461 cggccggctg agcgaccggg aggtggccca gtgcttcgag cacgcgcgga ccgacccggc
     8521 attcgatctg gcccggacgc tgccgccgcc gggcagcacg tagccgggag tgctcgctcc
     8581 cggggggaac cgagccggga aggggccggc acaggcggcg ggtgtggccc atcgagcgcg
     8641 tgcccctgcc tatggccgca gcgaatggcc gcgcagtgtg caagaagagg cgtcggaatc
     8701 gttcttcaaa gggaggcgca ggtgagcacg agcccatcgc gagacaccgt cgacgccgat
     8761 gtcgagcggc tgctgcgcgc cgcgctgaag ccgtccgccg gcggccgggc cggcgagtgc
     8821 cccgatgcgg agtcgctggc cgcgttcgtg gagggcaggc tgccgcctcg agagcgagcg
     8881 ccactcgacg cgcacttcgc ggcatgcggc cgatgccagg agacgctggc cgtgatgggc
     8941 gcggcgccgc cgctgccgct ggtcgcgccg gtgcggccgg ggtggttcgc gcggatcacc
     9001 gctccgaggc tgcgctggct tgtgccggtt agcgctgcgg ccgcagtggc ggcggtgttc
     9061 ttcgcgacga ggccggtcat agcgccggga gagcatgcgg ccacggccga agccccggtg
     9121 cagatggcgc acgctgcacc gcccgaactg gggccggcgg gcgcggcccg gaatgaggtc
     9181 ccggaggcgg cgctgcagcg ggacgcctcc gccaggaccg gcgcggctgc ggatgagctg
     9241 aaacccggag gtcctcggcg gccggccgcc gtgctggcag acgagggcgc agcgggagag
     9301 ggcggccggc aggctgtctc gctggggatg ccgacggcgg agcaggccgt gccggccgcg
     9361 gccgctatgc gcgagacgag ggacgtgcgt gaggtcgccg tgagcgaggg ggcgaagcct
     9421 gccgggttgg ccaagtccgc gacccagtcc gaggccgctg gtgccccgtt ggcggcgtcg
     9481 gtggcgtttc gcgagcgtga cgcagccggc gcgttgctgc ccgtcgttgc agcccctgat
     9541 ggagttgtgc agtggaagct gggcggagaa ggccgggtgt ggcgctcgga ggatggcggt
     9601 gttcgttggc gcgaacagcg tacgggcgtc ggggcggtgc tcacggcggg atcggcgatt
     9661 tcggccgacg tgtgctggat cgttggtgaa ggcgggacgg tgctgctgac ggtcgacggg
     9721 gcgttctggc agcagcggcc gtttccgttt ttggcggatc tcgtcgatgt ccgggccgcc
     9781 tccgcgcgat cggcggtcgt cacagcccgc gacggccgcc ggttcgagac ccgagacggc
     9841 ggcctcacgt ggagataagg ggtcgggacg ctttttgcaa acgacggatt tgtcatacag
     9901 atccgtcgtt tgcaaaaagc gtcccgaccc cagggaggtt cctatgatgg acacacaact
     9961 tcggaccgcc gtgctcgcgg cagctgcgat cctcactagt gccctcgcta gcggtccgac
    10021 gatcaccgcg cttgcgagcc aggattccgc cgccgccggc ggccagagcg acctggccgt
    10081 caccgtgtac aattcgagcc tcgccctcgt gcgcgacgtc cgcgagttcg ccctgccctc
    10141 gggcgagtcg gaccttcggt tcgtggacat cgcagcgacg gtcaaccccg cgacagtgca
    10201 cttccgttcg ctgacggacc cggctcgcgt cgccgtcctc gagcagaact acgagtacga
    10261 cctgctcgag cccgagaagc tcctccgcaa gtacgtcggc cgcgaggtca cgctcgtgcg
    10321 gacgggcgtg gagaacggca ccacgaagcg cgaagaagtg aaggcgacgc tcgtgagcct
    10381 gaacaacggc cccatctgga agatcgggaa cgagtacgtc accggcctcg gctcggatca
    10441 cctccgcttc cccgagctgc cgtcgaacct ctacagccgg ccaaccctcg tctggaagct
    10501 cctgaaccgc ggcggcccgc gccatcgcat cgaggcgtcg tatcttgcgg gcggcctgtc
    10561 ctggcagtcg gactacgtcc tcacggtcgg ccgcgacgac gcgtcggccg gcctcgacgg
    10621 ctgggtgacg ctcaccaacg agagcggcac cgcgttccgc gacgcgacgc tgcagttcgt
    10681 ggccggggat ctcaaccgcg tccagcccgc cgcgcgcaag cagatgctcg agatgatggc
    10741 cgatgccagg ggcgccgcgc ccgccatggc ccaggaggcg ttcgccgagt accacctcta
    10801 cacgctgggc cgccggtcca cgatcgagaa ccagcagacg aagcagctcg cgctgctcag
    10861 cggctcgggc gtgccggtcg agaagcgcta cgtcgtcgac ggccaggctt tctactaccg
    10921 caaccgccag agccccggcc gtccgctcaa ggacaaggtg caggtctact accgcttccg
    10981 caacgacgca cagagcggcc tcggcgcgcc catgccggcc ggcattctcc gcgtctacca
    11041 ggccgacgcg aaaggggggc tccagttcgt cggcgaggat cgcatcgatc acacgccgaa
    11101 ggacgagacg atcgacgtca agatcggcag cgccttcgac gtcgtgtgcg agcggaacca
    11161 gaccgacttc gagaaaatca gcgagctcgt ctacgagatg gagtacgccg tcacgctgcg
    11221 caaccacaag acgacgccgg taaccgtgcg ggtgaacgag ccgattggcg ggacctggaa
    11281 gatgctgcgc tcgtcgcacg cctgggagaa gacggcggcg tgggcggcgc agttcacggt
    11341 gccggtggcg gccgacggca cggccgtcct ggcctaccgg gtgcgcgtca cctactgagg
    11401 cttgcgggcc gccatgtcgg tgctggcggg gtcagatccg cggatttctg gctctgaccc
    11461 cggaattctg gatctgaccc cgaagccgaa gccccgaagc tcggagtcgg ctagactagc
    11521 tccaccgccc ggcgcccgcc gggccccgca gcgaggtgcc cagccatgtt cgacgccgcc
    11581 acctaccaga gccgccgccg ccgcctgagc aaggatgtcg gttccggcct gatcctgttg
    11641 ctcggcaacg acgaagtggg catgaactac gcggccaacg cgtaccactt caggcaggac
    11701 agcacgttcc tgtacttctt cgcggtgaac ctgccgggcc tggccgggct catcgacgtg
    11761 gacgccggca cggagacgct cttcggcgac gatctcacgg tggcggacat cgtctggacc
    11821 ggcccgcagc cgacgatcgc gaagctgtgc aagccggcgg ggatcgcgtc gagcgcgccg
    11881 atggccgagc tggagacgcg cctggcggcg gccatcgcca aggggcgccg cgtgcacttc
    11941 ctcaagccgt accgcgccga gcacacgctg aagatcacgg cgctgctcgg gctgaagccc
    12001 accatcgtcg cgtcgtaccg gtcggaggcg ctgcacgcgg cggtcgtcgc ccagcgcaac
    12061 gtcaagtcgg ccgacgaggt ggccgagatc gagcgggccg tcggcgtgtc gcgcgagatg
    12121 tacctggcgg cgatgcgggc ggccaggccg ggccggcgcg agtacgaggt ggtggcggag
    12181 atcgtccggg tccagaaggc gaacggctgc gaactggcct ttccgatcat ctgctcggtg
    12241 cacggcgaga cgctgcacaa tcacgcccac gcgaacctca tgaaggccgg cgacgtgatg
    12301 gtgctcgact cgggcgtgga aaccgaacgg ggctacgcga gcgacatcac gcgcacgatt
    12361 cccgtgggcg gccggttcac ggccgagcag cgggtgatct acgacctggt gctgaagacg
    12421 cagctcgcgg ccatcgccgc catcaagccc ggcgtgcggt acaaggacgt gcacctgctg
    12481 gcctcgagga gcttcgcgtc aggcctgaag gagatcggcc tcatgaaggg cgacccggcg
    12541 gaagcggtgg cagccggcgc gcacgctctc ttcttcccgc acggcctcgg ccacatgctc
    12601 ggcctggacg tgcacgacct cgagaacatc ggcgagcagc acgtcggcta tgagcccggg
    12661 ctcgagcgga gcggccagtt cggcttgagc tacctccgcc tcgcccgcac gctgaagccg
    12721 gggttcgtcc tgaccgtcga gcccggcctc tacttcatcc cgcagctcat cgaccagtgg
    12781 aaggccgaca agcgccacgc cgcgttcgtc aactaccgag aggtggagaa gttccgcaac
    12841 gcgcgaggct accggatcga ggacgacgtg ctggtgaccc ggtccgggaa ccgcgtgctc
    12901 gggccgccga ttccgaagaa ggcggaggac gtcgaagcgg cgtgccgcgc gtagcagcgc
    12961 gcggaaggcc ggacctcgcc cgagagaatc cgaagccagg gattcgcatg gagcccaggg
    13021 ccttcaggcc cgcggacgcc cgcggccgac cggcggcgtc attccccgac tggacgtcgg
    13081 ggccgtggat ctgaccccat ccgacggaga gcccaggcat ggccctgtcc tacacgcagt
    13141 ggcttcacac gcagtggccc gccggcacga tcgagccgcg cccggaaacg gaccgggacg
    13201 gccggacgtc cgtgcgtggc gttcgcgtgg ccggcgaact cgccggccac gtgctcctgc
    13261 gcgaggccgc ggactcgggc gcgcgggccg tgcgggccat cgcggcggag cgcgggttca
    13321 aaccgtcggc tgatccgagc gtgccggacc tcgccgtcat cggcgcgggc gcggccggca
    13381 tcgcggccgc catcgaggcc cgtcgcgccg gcctgtccgt cgtcgtctac gaagccgcgg
    13441 cgccgtttgc gtcgatcgcg gggttggcgc gcgggcgctc aatcgtgacc ccgactgccg
    13501 cggcgcctgc tcccgcgccg ggcgacggcg cgtctgccgg agccctggcc atcacggccg
    13561 gccggaagga ggacgtcctc gcagacctcg agcggcagcg ccgggaagca ggcatcgaaa
    13621 ccgaggcgag cctcgtcgag cgcatcgaat cccgcaaggg ggcggtggcg ctgcaccacg
    13681 ccggcggccg cgtcaccctc gcgcagcgcg ccatcgtcgc gatcgggcga agcggcgtct
    13741 tcccgcctct cggatgtccc ggcgacgacc tcgacaaggt ccgcacacgg atcctcgacc
    13801 ccaccgagca cgccggaaag cgcgcgctcg tcgtcggaga cggcgaccac gccatcgaga
    13861 cggccgtcgc gctggccacc tgcggcgcca aggtcacgct cgcgtgccgc ggcgcggagt
    13921 tcgatcgccc gcgcgcggag aacctcgtca agctccgacg catcaccgag cagccagaag
    13981 cccaggtgca catcgagtcg ccgagcgccg agcgcgtgac gaccgcgacc atgcggaaca
    14041 tgaagagcgc cgaagggccc ggatccctgc gcgtcctcat ggccacgacg gtggagcgcg
    14101 tcggccccgc agccgtcacc ctggtgggcg cggacggggc ggagcaggtc gtgcccaacg
    14161 acgtcgtgtt ctcgatgatc ggccgcgccg aaccggaagg gctcctccgg cgctccaggc
    14221 tgcgcctgcg cggcacgcgc gacgcggcgg tctggctcgg cctagccgtt tccatctgcg
    14281 tgttcgtgct tgtcctgcac tgggccgtca gccggccgga actccccatc cagcgggccg
    14341 tggcggccta ccgcgcgttc ccctacgacc tgcccggact cgtcgactcg gccggcggcg
    14401 cgctagcgga tctcgccgcc cgcgagacgc accttctcta caccctgaag tccagcctgg
    14461 gcacgccgct gttccacctc tccctcgcgt ggtgcctcgc cgtgacgatc ttcggcgccc
    14521 gccgcatcgc gaggcggcgg acgccgtacg tcttctggca gacgctcacg ctgattgcgg
    14581 tccagtggat cttcctgatc gtgatcccgc acctggccct gccgtgggcg gggcgaaacg
    14641 gctacttcga gccccgccag cccctgcgct ggatggccga tcagctcttc gagcgcggcg
    14701 acggcttcct cggccacgag cgcgcgtact ggagggccta cgggttcgtg ctgccgttcc
    14761 cgctgaacgt cttcaacgtg ttcaccgacc gcccgatgtg gctgtggatg gcgatcgccc
    14821 tgctccaggc gctcgtgggc gtcccgctga tggtgcggcg ctgggggaag ggcgggttct
    14881 gcgggtgggt gtgcccggcc ggcgcgctgg ccgagaccgt gggcgacgtt caccgcgaga
    14941 agatgccgca cggcccgctg gccaaccggg tgaacatgat cgggcaggga gtgctggcgc
    15001 tggtgctcgc gctgctggcg ctgcgcttcg cggcgtggtc gctcggcccg cgatcgtggg
    15061 cacagacggt cttccgggag gccttctcgg gcctgccgtt cttcaactac acgtggctcg
    15121 tcgtcctgtt catggccggc gccgtcggga tcggcgccac gttcttcttc agcggccgcg
    15181 tctggtgccg tttcgcatgc ccgctgggcg cgctgtcgca catccacgcg cgctcgtcgc
    15241 gcttccggat cctgcccgag aaggcgaagt gcatctcgtg cggcgtgtgc acgtccgtct
    15301 gccacgccgg cgtggacgtg atgaacttcg cgaacaagga ccagcccgtc cgcgacccgc
    15361 agtgcgtgcg gtgctcggcg tgcctcgagc agtgcccgac gggcgcgctg gccttcggcc
    15421 tcgtccgcaa gagcggcgag gtggtcttcg accgcctccg cgccggtcgg ggccaggcct
    15481 gaaaacaccg acgcactgct gccctcatcg cggcggcgcg ccaggatgtt caggcccgac
    15541 cccgcgtctc cggctgtcag cgcttcaggt acttgtcgaa ccaggccagg taccgctcgt
    15601 agcggtcccg cacgtagctc ggcttcgtca agccgtggaa ctgctcgggg tagatgacga
    15661 gctcggtgtc gacgccgagg cttctgagcg cctggtacat ctgctcgctg ccggcgaggg
    15721 gcacgttgaa gtccttctcg ccgcacaaga acagcgtggg cgtcttgatc cgatcggcgt
    15781 gccagaaggg gtaggacacc ttgaggtaca gatcctgcgc cttccacggc tggccgatct
    15841 ccgtatccca ctggacgatg tagtggtcga ctccgtacat gctggactgg agcgcgctgc
    15901 ccgcgccgct gaccgccgcc ttgaagcgcg tgtcgctggc aatcgtgtag tcggtcagca
    15961 ggccgccgta actccacccg ccgatgccga ggcgctccgg gtccgcgacg cccgaggcga
    16021 cggcctgatc gacggcgccg aggagatcga cgacctcctt gttgccccag tcggcgtaga
    16081 tggccttctg gaaggcctct ccgcgccccg agctgccccg gtagttgatg ttcagcacca
    16141 cgtacccgtt cgccgcatag agctcgttct cgaaggtgaa cgagtagtcg tcctggccgt
    16201 tcggcccgcc gtggatgatg agcagcgtcg ggtacttggt gcctgcggcg tacgacgccg
    16261 gcttcacaat gaggccgttc accacggtcc cgtccttgct cgtcgacgtg aagtcctcgg
    16321 tcgtcgccag ctgcagctgg gcgaacagct cgtcgttcac cttcgtcagc tggcgcagcg
    16381 tgccgttctc gagggcgtgc acctccggcg tcgccgtgga cgttccggcc agcagcgtga
    16441 ggcggccgtc ggcgccaagc gagacgtcgg acacggtccg gcgcccggcg gtgagcttct
    16501 cgacctgccc gcccgcgacg ctcgtgcggc cgacgtacga cgccctgtcg tcctcgacgg
    16561 tgaacgtcag catccggctg tcgggcgacc acgagatggg ccctccaacc ggccggtcga
    16621 gggcggccgt cagcaccttc gccgccccgc ccgcggcggg gacgatggcg agcttgttga
    16681 tctcgtaggc gaggtacctg gtctcgtcgc cctggaagta ggcgatccac cggccgtcgg
    16741 ggctccacgc cgggcgcccg gcatcggccg ccgtcgacgt cgtgacctgc ctcggctccg
    16801 cgcccggctt cgcgtcgacg acgtagatgt ccgagttctc atggcgatcc gggtccggcc
    16861 ccgtgcgcgt gctcacgaag gcgatcttcg tcccgtccgg cgaccaggac ggctccgagt
    16921 cgtcgaacgg cccggacgtg acctgctcgg ccttcttcgc ggcgatgtcg aacacgtaga
    16981 cgtggctgta gaggcgcttc aaatagccgt cccggtcccg cttgaagcgg taccggtcga
    17041 ggacgatggg cgggagcgtc ttgcgcttcc agccctccat cttctcgggc tcgtcggccg
    17101 ggtcttggtc gcttttcgtg aaaacgaagc gcttgctgtc gggcgaccac gagtactcgt
    17161 cgacgccgcc ctcgatctcc gtcagtttct gcgcctctcc gccgaggcgg tcgagcagcc
    17221 agatctgcgc gcccttcttc ttctcctctt ccgtgccgcg gctggagagg aaggacaggt
    17281 agcggccgtc ggggctccag cgcggggcgc tctccttctc cgggcccgac gtcagccgga
    17341 ccgtctgcgc gccgtcccag ctcaccatcc agatatcgct gtcgcgcttg tccttctcga
    17401 ggtcggccga cgtcaccacg taggcgaccc acttcccgtc gggcgagcgc tgcgggtcgc
    17461 cgaccgactt gatcttcgcc agatcgtcga gcgtgatggc gcgcctggcc gcgaccgcgg
    17521 gagcttgagc ggcgggcccg gccgccaggg tgacggcgac aaggacggcc gcggcggccg
    17581 ccagggcgac ggtgcgggtg agtctgggca tctttggcgt cctgcaatga cgacggcagc
    17641 ccggaggcgg cctgagttgc ggcatagtag cacgcaagag tccacgggcc cgctgggcct
    17701 tactgtcgga ctccacaggg gatgccctgg atcgccagcc ggcctctcgc gcccgggttc
    17761 gcgggggccg cgcgtctcgt cccgcccggg aacgaaggag ccgtcgccca tgacacgcag
    17821 cccaggcgcc cgcgccgccc gtgtttcgct cgccctcgct ctcgcgctcg ctccgctcag
    17881 cgtctccgca gccggcgcgg cagggccgaa aggaccagga ggtcaagcgg ggtcacacag
    17941 tccggcaggt cgagcaggat caaccgtggc ggcgggcgcg gccgagc
//
LOCUS       DAOSIF010000009        17695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_3409_length_17695_cov_4.565986, whole genome shotgun sequence.
ACCESSION   DAOSIF010000009 DAOSIF010000000
VERSION     DAOSIF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17695
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3409_length_17695_cov_4.565986"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLT35_00785"
     CDS             complement(<1..202)
                     /locus_tag="PLT35_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27787.1"
                     /translation="MEPSAFNLAVPVSTGDVFLMNTLTDAQVLVSRDVAALVEHLSAG
                     SGGDGGTAGRAADAGEALEELAS"
     gene            complement(406..1806)
                     /locus_tag="PLT35_00790"
     CDS             complement(406..1806)
                     /locus_tag="PLT35_00790"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG27788.1"
                     /translation="MTPGSRTILVVDDDEGMRDTLTAILRRDCRVLRAATGESALALL
                     AREAIDVVLLDVRLPGIGGFDVLREIKEAQRLVEVIVISAINDVETAVQAMKLGAFHY
                     ITKDFEYDAVRSLVRNAADRALLTRKVMSLSAQVADDRNRPFVVGPSRSMRELLDLVH
                     KVAALPATVLILGESGTGKELLARMIHRESGRADAPFMPVNLAAIPADLVESALFGHE
                     KGSFTGAIRQQLGKFELADGGTLFLDEVGELRPDVQAKLLRAIQESEIERVGGSRPIR
                     VDLRLVAATNIDLEKAVREGRFREDLYYRLKVIPMKVPPLRDRPEDLPALAQFFVGRF
                     NAKFRKRVLEIEEPAMEALKAHSWPGNIRELENLIERLVAVSDGDRIGPADLPYEYHL
                     AHLSRPAPGGAGLFDRACETFERSFILKALERERWNVTATARALGMPLSTLKFKMDRL
                     DLREAARRMRGAWTGS"
     gene            1963..3378
                     /gene="lpdA"
                     /locus_tag="PLT35_00795"
     CDS             1963..3378
                     /gene="lpdA"
                     /locus_tag="PLT35_00795"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOG27789.1"
                     /translation="MANAFDVIVIGAGTGGYPAAIRAAQLGLRVAVVERRPTLGGTCL
                     NVGCIPTKALLEHAHALKVAKEAKTWGLVLGDQPARIDMAQVQARKDKIVTILTRGVE
                     FLFKKNNVTWIKGSGRIAGPGTVEVTGDQPRTLTAPQIVIATGSHARGVPGIEIDRAR
                     IITSDEAIALREVPKSIVILGSGPIGVEFATVFKRFGSDVTVVELLPRLVPLEDEAIS
                     LELEKSFKKQGIAFRVSTTVTAARAGAEKVEIDAKLPDGSSATLAAEYLLVATGRGPV
                     TSGLGAEEAGIRMDRGYIVVDEQFRTSVPGVSAVGDVITTGKPGHPQLAHLSTAEGIA
                     LAERLAGKEFRPINYDHVPKCTYSDPEIGSVGLTEQQAIERGHDVRVGTFPFGVLGRA
                     RIANETDGLVKIVAEKKYDEILGIHMIGPRATELVSEATLALRMEVTVEELIRTIHAH
                     PTMAEAIGEAAHATHGAAIHV"
     gene            3505..4776
                     /locus_tag="PLT35_00800"
     CDS             3505..4776
                     /locus_tag="PLT35_00800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOG27790.1"
                     /translation="MPDVLMPQLGESIAEGTIVRWLKRPGERVDRDEPLFEISTDKVD
                     AEIPSPEAGVLVEVRAREGETVPVNSVVAVIADEAPGGGPPAPAIEAAGPDGEPAPAR
                     REAAEASPGAGAALPGAAAASAAGGEAGRKGRKSSPLVRRIAREHGVDIDAVPGTGLG
                     GRVTKADITGFLEQGRISAPAAAGAARARQDAPAAGLRVVPMSVMRRKIAEHMVRSRR
                     TSAHAHSVFEVDYSAVAAIRAARKADYERQGVKLSYLSFVMKAASGALKAFPVLNASL
                     DGSDIVYREDINLGVAVALDWGLIVPVVKGAGDMSVMELSRAVQDLAARARAKQLRPE
                     EVQDGTFTITNPGSYGALFGTPIINQPQVAILDVGAIEKRVSVVGDAIAIKLKGHLAL
                     GFDHRLIDGATADRFMSHVKRALESFDADGV"
     gene            4766..5437
                     /gene="lipB"
                     /locus_tag="PLT35_00805"
     CDS             4766..5437
                     /gene="lipB"
                     /locus_tag="PLT35_00805"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOG27791.1"
                     /translation="MGSDAPRALAVKRLGVVRYGDGLALQQALVEQRAAGEIDDTLVL
                     LQHPHVLTLGAKAAQSRSHVLATPQRLEELGVELHETGRGGDVTYHGPGQIVGYPILN
                     LKPDRCDVHRYVRDLEEVMIRVTAAYGFRAARIPGLTGAWVGAEKIGAIGVRLQRWIT
                     SHGFAFNVSTNLDFFRLIVPCGISDRGVTSLERLAGRAIPVAEVEDRVIREFASVFSR
                     RPRET"
     gene            5616..6215
                     /locus_tag="PLT35_00810"
     CDS             5616..6215
                     /locus_tag="PLT35_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG27792.1"
                     /translation="MRAGDESAVVDLDAAYRSRIQQLALRYVRNPDDAEDVAQDVLLK
                     VHLKIGAFRGDAALSSWIYRITFNTVMSRLRSERAASRHEIREHEPPAGVAESGGQAR
                     REPADWSSMGDEAMLRAQLRQRLSAALGDLPEIYRTPVILRDLQGLSTEEASEHLGIK
                     EQTLKSRLHRGRLFLRRRLAEFASGVSLHRRASDFSFAT"
     gene            6352..8931
                     /locus_tag="PLT35_00815"
     CDS             6352..8931
                     /locus_tag="PLT35_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOG27793.1"
                     /translation="MSEFIAHYEILGEIAAGSLGAMYRARDTRAGRTVAIRIVKAGMA
                     DPARRARALDLIQPFTAITHPHVATLFEAGERRGAIYLVYEYIPGEKLAAVIGGHPMN
                     LRRALDLGEQVADALAEAHSLDLVHGSLTPASIVVTPKGHAKVLDFGLCAGVARDPLR
                     RIGEAQEGPPGPGALDRLELPWVAYTAPEVLAGGPPGARSDLFALGAILHEMLTGRRA
                     FPGRSSSDVVEQIRGAHPARPSLLNHSLPRELDAIVDKALAKDPERRCQAAAELAASL
                     REAAAAAPGGAAAPAPTRRHQSARRAAPRRVAAALFLAAAVGASAWHWRTPLRQAWQA
                     RFGARPEPRLVVMPFQVTGAAGAERPYFGAGFAEGLAKGLGRVPGVVVLGRSMVRSPA
                     GQSPRGAVAAAGAAAAVLGSVTPSDEDWTSLRIDARLVEAGTGRTAWSGAFTTGIADA
                     MALQARMAVEIASRLDLSGAKAAGQGRAALLLVKPGAYDAYLRALDAMASNDASRAAQ
                     LFEAAADEDPGLADAQAGLALALFVASAFEGRGHFGDVQTHARHAAEAACAADPDSPS
                     AWLSMGLTAPTISGAVAHLKRALELDASEAHAYLALAGILRPVDPDAALRFARRAGEL
                     DPGQPLAVLIEAAAHLSAGRPAEAVAAAERGRSLFSELPWWDAVCLRARLGRGPGPAG
                     APPPGTQPWRDAGDFPPGTILRAAELAGAGRTGDALTLLGSLTRLHPGSCEARAMTAA
                     VLLRAGRMGDAQRLASAVWSRAAAAPDGSGWSRCAYMAAAALNEPARAAAWLARAASS
                     ERELRGWGAVSGVLDGLAATRDGVFPWSNVSAAPAVAESAASLRAAMDRTRAETARVL
                     QGS"
     gene            8945..9589
                     /locus_tag="PLT35_00820"
     CDS             8945..9589
                     /locus_tag="PLT35_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG27794.1"
                     /translation="MLIESRAAAPFHKNGFLVACDGSREAVLIDPGDEVEALLAIAGA
                     RRLSIGAILLTHGHVDHVSGIAAAKRATGAPVGLHREDLFLYENAVEQGRFFGYRVEP
                     PPPPDFFLAEGAPLSFGCCAVDAHHTPGHSPGGVCLRVRCPEEPGTHLFVGDTLFAGS
                     IGRTDLPGGDYETLMRSLRTVLFGFGDAAVVHPGHGPDTTIGRERATNPFLRSA"
     gene            complement(9591..9890)
                     /locus_tag="PLT35_00825"
     CDS             complement(9591..9890)
                     /locus_tag="PLT35_00825"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOG27795.1"
                     /translation="MWELRARRTDPARPVSFRLMPGAVKTIGRARAADFCLDVPFVSR
                     LHCRVEVGPDGAVEVVDLGSTNGTWVDGKRVSRATLARGSVLRVGRVEFALEPCG"
     gene            complement(9862..11571)
                     /locus_tag="PLT35_00830"
     CDS             complement(9862..11571)
                     /locus_tag="PLT35_00830"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG27796.1"
                     /translation="MIRRTLALAALIAAAAGPAAEGQAQQPEPAAVRFVVFMAGRQLG
                     FERAQVRATADGWTLSGSGRLADPVDLRTGEFQVRYDRDWRPLGAEISATLQGEPSVT
                     KTTFANGSATSEITRRGKSSWKVDAVSADPVVLPPKAFFSAFEALALRLRTAPVPAAL
                     RGYIVGQGEIAIAVTAVGDERFQTMDGAVDSRRYTLTIEESSGPVEAELWANREGRLL
                     RFRVPANALEVSREDVAAVSTRVERLARPNDEAITVAAFGFNLAGTLSKPASVPAAAG
                     RAGGKAPALPAVVLVSGGGFADRDENVAGVPVFAQLAGALADAGFAVVRYDKRGLGQS
                     GGRSESATAGDYAEDARAVVRHLRKRRDIDPRRIALVGYGEGGFVAMLAAAGAKDDVA
                     ALALMAAPGSKGSDLVMEQQLRLIEKMALPDAERQQRVELQERLHHAVITGTGWDTVP
                     PGYREQADTAWFRSLLLFDPARVMEKVRQPVLVVHGDIDRQVPARHGALLIEAARARK
                     RPLPAALVTIQGINHLLVPAATGEPDEYATLPDRRVSPKVPEALVPWLKDTLHVGAAG
                     PAH"
     gene            11665..12657
                     /locus_tag="PLT35_00835"
     CDS             11665..12657
                     /locus_tag="PLT35_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOG27797.1"
                     /translation="MTIPDDYTAARLAAAVAGLGHRDRIVVRGADRKSFLHAVLTNDI
                     AGLASGTGCYAAFLTPQGRMVADMNLFDLGDAALIDTPAGAGQRLLERLDQLIFSEDV
                     QLGDLSEAWGCLGVYGPRAAEAAGAAIGADLAAFAPFQNARLAFRGDTLVAARRDGLG
                     LTGFLLFAAAGQVEALEAALAEAGAVRMSAATAEVLRVEAGEPAFPADMDEATIPLEA
                     GIEGRAVSFTKGCFPGQEVLVRIRDRGHGRIARKLVGVALDGSSVPSPGSPILAGDKE
                     AGRVTSAVMSPALGRPVALGYVHRDLAEPGTAVAVASGAGSIPGTVTALPFVTP"
     gene            complement(12803..13192)
                     /locus_tag="PLT35_00840"
     CDS             complement(12803..13192)
                     /locus_tag="PLT35_00840"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOG27798.1"
                     /translation="MKRVVVAAAVIERGGAFLLARRLEGTHLAGHWEFPGGKLHEGET
                     LEECLAREIREELDAAIEVGEEILATIHDYPDRSVELRFFRCLLLGEPRPVMGQEMRW
                     VARADLGAVPLPPADAELAGLLISRSV"
     gene            complement(13189..13947)
                     /locus_tag="PLT35_00845"
     CDS             complement(13189..13947)
                     /locus_tag="PLT35_00845"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOG27799.1"
                     /translation="MPPPARRRAFRRALLRWYDRNGRDLPWRRTTDPYHILVSEIMLQ
                     QTQVHRVLPKYHEWLGKYPTLEALAGAPEEGAVETWRPLGYNIRPRRLHAIAREAVSR
                     FGGALPADEAALRSFKGIGPYTAGAMLSFAFGKRAPILDTNVARVLFRVVVGRGEAGS
                     HRMRKHLWALSEAMMPRKRVFDFNQALMDFGALVCAARNPKCGECPMAGFCEAFRLGA
                     RGSGLGARPAAPKPTVRPSGEGGRSGKAAGVTRR"
     gene            14039..14575
                     /locus_tag="PLT35_00850"
     CDS             14039..14575
                     /locus_tag="PLT35_00850"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG27800.1"
                     /translation="MEKIYTRTGDGGATTLFDGTRIAKSDPRVDAYGEVDEANAAVGQ
                     ARAAGLDEDLDRMLAQIQRDLFALGSRLADPAERIAGRVTKVALGPDDVTRLEGWIDA
                     CAEELQPLRRFILPGGSPAAASLHLARTVCRRAERRMVALGVLEVEFLAYMNRLSDLL
                     FVLARVVNRRAGVAESEW"
     gene            14629..15801
                     /gene="thiI"
                     /locus_tag="PLT35_00855"
     CDS             14629..15801
                     /gene="thiI"
                     /locus_tag="PLT35_00855"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOG27801.1"
                     /translation="MTSILVHYQEIALKGRNRPYFIGQLVRNVRRQTADLGVKDVRAL
                     MGRIEIVLGPSAGYEAVSERLRHVFGIANFSKAGRAARDLESVASAVLADLEGHRPVT
                     FRVSAKRGDKTFPMTSPQLERELGARIVERFGWPVKLERPEMVVTVEVLPNRIFYSFG
                     KEKGAGGMPTGVSGRVACLMSGGIDSPVAAYRLMRRGCSVVPVHFHSHPFVSRASQEK
                     VRELCEVLTRYQMHTTLRLVPFGELQRQVVLSVPAPLRVVIYRRLMLRIAERIARASR
                     AKALVTGEVIGQVASQTLENLTVIADATSMPILRPLVGTDKEEIVAEAMRIGTYEISI
                     VPDQDCCQLFTPRSPETHARRWQVEEAEAKLPIEDMVRAAVEATATEEFRFPPPTS"
     gene            complement(15791..16261)
                     /locus_tag="PLT35_00860"
     CDS             complement(15791..16261)
                     /locus_tag="PLT35_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27802.1"
                     /translation="MTRMRRMAVAAALAAAIGTAAACVETRHENPASPTPALAVLSAG
                     SWTSSTAAVASSLDPKACGNLEWTITSLTGTSASGTFKAVCGGGLTLEGSAEGKLNGL
                     TADLRVSGTVAGAGMACTFSLVGTALPEGLDRVRINYSGSTCLGPVSGSEVIRK"
     gene            16677..17228
                     /gene="def"
                     /locus_tag="PLT35_00865"
     CDS             16677..17228
                     /gene="def"
                     /locus_tag="PLT35_00865"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG27803.1"
                     /translation="MSLLKISRMGHPILRKKMKAVAPSSIATPAFQKLIDDMALTMLE
                     NDGAGLAAPQVHEDCRLFVARVFNDREDDENAEPAEPEILALVNPEIVPASRQIEEDW
                     EGCLSIPGLRGLVPRYRDIRVKAHDRNGERIDLQASGFMARVIQHETDHLDGILFLDR
                     MKSMQSLSFIEEFAKYHAKRERE"
     gene            17383..>17695
                     /locus_tag="PLT35_00870"
     CDS             17383..>17695
                     /locus_tag="PLT35_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG27804.1"
                     /translation="MKLQPVAIVFATVFIDLLGFGIIIPLLPFYAETFGATAFTVGML
                     ATSFSLMQFLFAPFWGRLSDRVGRRPVILVGLLGSSLAYLAFGLASTLTTLFAARVFA
                     GI"
BASE COUNT         2498 a         6363 c         6302 g         2532 t
ORIGIN      
        1 ggctggcgag ttcctcgagc gcctcgcccg cgtcggcggc ccggcccgcc gtcccgccgt
       61 cgccgccgct gccggcgctc aggtgctcca cgagggccgc gacgtcgcgc gacacgagaa
      121 cctgcgcgtc ggtgagcgtg ttcatcagga acacgtcacc cgtcgagacc ggcacggcga
      181 ggttgaacgc cgatggctcc atgcagaccg gggctccgcg ctggaccgag caaggagcgt
      241 tccagcgggg cccgagccgg gaacgtgcgg gattccgcgt gttatggctg gagtggcggt
      301 gtcctcaggc gagacaggtg gcaggaattg cagcggcggt tcgaggcacc cgttgcagat
      361 tctgcaacgc ggcgccgcgg cggcgcacag gccgccgaga gcggcctagc ttccggtcca
      421 ggcgccccgc atcctccgcg cggcctcgcg gaggtccagg cggtccatct tgaacttcag
      481 cgtgctgagg ggcatgccca gggcgcgcgc ggtggccgtg acgttccagc gctcccgctc
      541 cagcgccttc aggatgaagc tccgctcgaa cgtctcgcac gcgcggtcga acaggcccgc
      601 gccgcccggc gcgggccgcg acagatgcgc gaggtggtac tcgtagggca gatcggcggg
      661 gccgatgcgg tcgccgtcgc tgacggccac caggcgctcg atcaggttct cgagttctcg
      721 gatgttgccg ggccacgaat gggccttgag cgcctccatg gccggctcct cgatctccag
      781 cacccgtttg cggaactttg cgttgaagcg gccgacgaag aactgcgcca gcgcgggcag
      841 gtcttccggg cgatccctga gcggcggcac cttcatcggg atgaccttca gccggtagta
      901 gagatcctcg cggaaccggc cttcgcgcac ggccttctcg agatcgatat tggtggcggc
      961 gaccagccgc agatccacgc ggatgggcct cgatccgccg acgcgctcga tctccgactc
     1021 ctggatggcg cgcagcagct tggcctgcac gtccggcctc agctcgccga cctcgtcgag
     1081 gaacagcgtg ccgccgtcgg ccagctcgaa cttgccgagc tgctggcgga tcgcgccggt
     1141 gaaggagccc ttctcgtggc cgaacagcgc cgattcgacc aggtccgcgg gaatcgccgc
     1201 gaggttcacc ggcatgaacg gagcgtcggc gcggccggac tctctgtgga tcatccgcgc
     1261 cagcagctcc ttgcccgtgc cgctctcccc gaggatgagc accgtggccg gcagggccgc
     1321 gactttgtgg accaggtcga gcagctcgcg catggaccgg ctggggccca cgacgaacgg
     1381 ccggttccgg tcgtccgcca cctgcgcgct gagcgacatg accttgcgcg tgaggagggc
     1441 gcgatcggcg gcgttccgca cgagggaccg gacggcgtcg tactcgaaat ccttggtgat
     1501 gtagtgaaag gcgcccagct tcatcgcctg cacggcggtc tcgacgtcgt tgatcgccga
     1561 gatcacgatg acctcgacca ggcgctgcgc ctccttgatc tcgcgcagca cgtcgaagcc
     1621 cccgatgccg ggcaggcgga cgtcgagcag caccacgtcg atcgcctcgc gcgcaaggag
     1681 ggcgagcgcg ctttcgcccg ttgccgcgcg cagcacgcgg cagtcgcggc gcaggatggc
     1741 cgtcagggtg tcgcgcattc cctcgtcgtc gtccacgacg aggatcgtcc tggagccggg
     1801 agtcatgggc cgccttcgcg tccggagccg cgctgattgt aggcgccgcc ccgcggcgag
     1861 ttcaaggccg gggcgcggcg gcgggcccgc ccggcgcggt tgacagtcgc gggaggctgc
     1921 cggtaacgtg cagtctgcgg ctgctttcca ggagaacgat ccgtggcgaa cgcgttcgac
     1981 gtcattgtca tcggcgccgg caccggcggc tacccggccg cgatccgcgc cgcccagctg
     2041 ggcctgcgcg tggcggtggt cgagcgccgg ccgacgctcg gcggcacctg cctgaacgtc
     2101 ggctgcatcc cgacgaaggc gctcctcgag cacgcccacg cgctcaaggt ggcgaaggag
     2161 gcgaagacct ggggcctcgt cctcggcgac cagcccgccc gcatcgacat ggcccaggtg
     2221 caggcgcgca aggacaagat cgtcaccatc ctcacccgcg gcgtcgagtt cctcttcaag
     2281 aagaacaacg tcacctggat caagggcagc gggcggatcg ccgggccggg gaccgtggag
     2341 gtcacgggcg accagccccg gacgctgacg gcgccgcaga tcgtcatcgc gaccggatcg
     2401 cacgcgcgcg gcgtgcccgg catcgagatc gaccgcgcgc gcatcatcac gagcgacgag
     2461 gccatcgccc tccgcgaggt gccgaagtcc atcgtcatcc tcggaagcgg gccgatcggc
     2521 gtggagttcg ccacggtgtt caagcggttc ggcagcgacg tgaccgtcgt cgagctgctg
     2581 ccccgcctcg tgcccctcga ggacgaggcg atctcgctcg agctggagaa gtcgttcaag
     2641 aagcagggca tcgccttccg cgtgtccacg accgtgaccg cggcccgggc gggggcggag
     2701 aaggtcgaga tcgacgcgaa gctgcccgac ggatcgtccg cgacgctcgc cgccgagtac
     2761 ctgctggtgg ccaccggccg gggcccggtg accagcgggc tgggcgccga agaggccggc
     2821 atcaggatgg accgcggcta catcgtcgtg gacgagcagt ttcgcacgag cgttcccggc
     2881 gtgtcggccg tcggcgacgt catcacgacg ggcaagccgg ggcacccgca gctcgcgcac
     2941 ctctccaccg ccgagggcat cgcgctggcc gaacgcctgg cgggaaagga gttccgcccg
     3001 atcaactacg accacgtgcc gaagtgcacg tattcggatc ccgagatcgg cagcgtgggc
     3061 ctgaccgagc agcaggccat cgagcgcggg cacgacgtgc gcgtgggcac gttcccgttc
     3121 ggcgtgctgg ggcgcgcgcg gatcgccaac gaaacggacg gcctcgtgaa gatcgtggcg
     3181 gagaagaagt acgacgagat cctgggcatc cacatgatcg gcccgcgcgc cacggagctc
     3241 gtgtccgagg cgacgctcgc actgcgcatg gaggtcaccg tcgaggagct catccgcacc
     3301 atccacgcgc acccgaccat ggcggaggcc atcggcgagg cggcccacgc gacgcacggg
     3361 gcggcgatac acgtgtagcg gggaggaatc cggaattcgg aatcccgaat gcggatgtcc
     3421 ccgcggagca agcgctcggt attccgaatt ccgaattccg gcttccggat tccgtttcga
     3481 gcgcgaagcg gtgcgagcgg cgagatgccc gatgtcctga tgccccagct gggcgagtcg
     3541 attgcggaag gcacgatcgt caggtggctc aagcggcccg gcgagcgcgt ggaccgcgac
     3601 gagccgctct tcgagatctc caccgacaag gtcgatgccg agatcccctc gccagaggcc
     3661 ggggtgctcg tcgaagtccg ggcgcgcgag ggcgagaccg tgcccgtcaa ctcggtggtg
     3721 gcggtgatcg ccgacgaagc gcccggcggc ggcccgcccg cgccggcgat cgaggctgcg
     3781 gggcctgacg gggagccggc tccggcccgg cgggaagcgg ccgaggcatc acccggcgcg
     3841 ggagccgcgc tgccgggggc ggcggcagcg tccgcggcgg gcggcgaggc aggccggaag
     3901 ggccggaaat cgtcgcccct cgtgaggcga atcgccaggg agcacggcgt ggacatcgac
     3961 gccgtcccgg gcaccgggct gggcgggcgc gtcacgaagg ccgacatcac tggcttcctg
     4021 gagcagggac ggatctcagc gcctgcagcc gccggcgccg cgcgggcccg gcaggacgcc
     4081 ccggccgccg ggctccgcgt cgtcccgatg tcggtcatgc ggcggaagat cgccgagcac
     4141 atggtgcgca gccggcgcac gtcggcgcac gcgcactcgg tgttcgaagt cgattactcg
     4201 gccgtggcgg cgatccgggc ggcgaggaaa gccgactacg agcgccaggg cgtgaagctg
     4261 agctacctgt cgttcgtcat gaaggccgcc tccggcgccc tgaaggcgtt cccggtgctc
     4321 aacgcctccc tcgacggcag cgacatcgtc taccgggagg acatcaacct cggcgtcgcc
     4381 gtcgcgctcg actggggcct gatcgtgccc gtcgtgaagg gcgcggggga catgagcgtg
     4441 atggagctga gccgcgccgt ccaggacctg gccgcgcggg cccgggccaa gcagcttcgg
     4501 ccggaggaag tgcaggacgg caccttcacg atcaccaacc ccggcagcta cggcgcgctc
     4561 ttcggcacgc cgatcatcaa ccagccgcag gtggctatcc tcgacgtcgg cgccatcgag
     4621 aagcgggtgt cggtcgtcgg cgacgccatc gcgatcaagc tgaaagggca cctggcgctc
     4681 ggcttcgacc accgcctgat cgacggcgcg acggccgacc ggttcatgtc gcacgtgaag
     4741 cgcgcgctgg agtcgttcga cgccgatggg gtctgacgcg ccgcgggcac tcgccgtgaa
     4801 gcggctcggg gtcgtgcgct acggcgacgg cctcgcgctc cagcaggcgc tggtggagca
     4861 gcgcgcggcg ggggagatcg acgacacgct cgtgctcctg cagcacccgc acgtgctgac
     4921 gctgggcgcc aaggccgcgc agagccggtc gcacgttctc gcgacgccgc agcgcctcga
     4981 ggagctcggc gtggagctgc acgagaccgg gcgcggcggc gacgtgacct accacggccc
     5041 cggccagatc gtcggctacc cgatcctcaa cctgaagccg gatcggtgcg acgtgcaccg
     5101 ctacgtgcgc gacctcgagg aggtcatgat ccgcgtcacg gcggcgtacg ggttcagggc
     5161 cgcgcgcatc cccggcctga ccggagcgtg ggtgggcgcg gagaagatcg gtgccatcgg
     5221 cgtccgcctc cagcggtgga tcaccagcca cggcttcgcg ttcaacgtga gcacgaacct
     5281 cgacttcttc cgcctgatcg tgccgtgcgg gatctcggac cgcggcgtca cctcgctcga
     5341 gcggctggcc ggccgcgcga ttcccgtcgc ggaggtcgag gaccgcgtca tccgggagtt
     5401 cgcctcggtc ttctcgcgcc gcccgcgcga gacctaggcc ttcgcggttc gtcttccacg
     5461 ccccggcccg cagggccttg cgagccggaa caactccgcc tataatagtc ggagtttgag
     5521 ccctgtgcgc tctggctatg agcccgggag aagagagcac cggcatggaa gccctctcga
     5581 ccacctcgcg gacggatgaa ggcctcgtgg caagaatgcg ggccggcgac gaatcagccg
     5641 tcgtcgatct cgatgcggcg taccggtcac gaatccagca gctcgccctt cggtacgtcc
     5701 ggaaccccga cgacgccgag gatgtggcgc aggacgtgct gctgaaggtc cacctgaaaa
     5761 tcggcgcgtt ccgcggcgac gcggcgctgt cgtcgtggat ctaccggatc acgttcaaca
     5821 ccgtgatgtc gcggctgcgg agcgagcgcg cggcgagcag gcacgagata cgggagcacg
     5881 agccgccggc cggcgtggcg gagagcggcg ggcaggcgcg ccgcgagccg gcggactggt
     5941 cgtcgatggg agacgaggcc atgctccggg cccaacttcg ccagcggctc tcggcggccc
     6001 tgggcgacct cccggaaatc taccgcacgc ccgtgatcct gcgcgatctg caggggctgt
     6061 ctaccgaaga agccagcgag cacctgggca tcaaggagca gacgctcaag tcgcgcctgc
     6121 atcgcggccg cttgttcctg cgccggcgcc tcgccgagtt cgcgtcgggc gtgtcactgc
     6181 accggcgagc ctcggacttc agcttcgcga cttagcggcg gccccgcagg cgcccggagc
     6241 cccgtgctat cgtggacgtc cgctcgggcc gatagccgcc acagcggagc atgcggggcc
     6301 ccgagcgccg cgcgtcggga ccgggccttc gtccgcccgc gccagtgcgc catgagcgag
     6361 ttcatcgccc actacgagat cctcggcgag attgccgccg gcagcctcgg agcgatgtac
     6421 cgggcgcggg acacgagggc cggccgcacc gtcgccatcc ggatcgtcaa ggcggggatg
     6481 gccgatcctg cgcggcgcgc gcgggccctc gatctgatcc agcccttcac cgcgatcacc
     6541 cacccgcacg tcgccacgct cttcgaggcc ggtgagcgcc gcggggcgat ctacctcgtg
     6601 tacgagtaca tcccgggcga gaagctcgcc gccgtcatcg gcgggcatcc gatgaacctg
     6661 cgccgggcgc tggatctggg cgagcaggtg gccgacgccc tcgccgaagc gcattcgctg
     6721 gatctcgtgc acgggtcgct cacgcccgcc tcgatcgtcg tgacgccgaa gggccacgcc
     6781 aaggtcctgg acttcggctt gtgcgccggc gtggccagag accccctgcg ccggatcggc
     6841 gaggcgcagg aagggccgcc cggcccgggc gccctggatc gcctcgagct tccgtgggtc
     6901 gcgtacacgg cgcccgaagt cctcgccggc gggcccccgg gcgcccgctc cgacctgttc
     6961 gcgctgggcg cgatcctcca cgaaatgctg acgggccggc gcgcgtttcc cgggcgctcg
     7021 tcctcggatg tggtggagca gatccgcggc gcgcatccgg caaggccgag cctcctcaat
     7081 cacagcctgc cgcgcgaact ggacgccatc gtcgacaagg cgctggccaa ggatcccgag
     7141 cgccgctgcc aggccgccgc cgagctggct gccagcctcc gcgaagcggc ggcggccgcg
     7201 ccgggcgggg ccgccgcgcc ggcgccgacg cggcgccatc agagcgcgcg ccgcgcggcg
     7261 ccgcgccggg tggctgccgc cctgttcctc gccgccgccg tgggcgccag cgcctggcac
     7321 tggcggaccc cgctgcggca ggcgtggcaa gccaggttcg gcgcgcggcc cgaaccgcgc
     7381 ctggtggtca tgccgttcca ggtcacgggc gccgcgggcg cagagcggcc gtatttcggc
     7441 gccgggttcg ccgaaggcct cgccaagggg ctcggccgcg tgccgggagt cgtggttctc
     7501 ggcaggtcga tggttcgatc gccggccggt cagtcgccgc gaggggctgt cgccgccgca
     7561 ggggccgccg cggcggtcct cggctcggtg acgccgtcag acgaggactg gacctcgctg
     7621 cgcatcgacg cgcgcctcgt ggaagcgggc acgggacgga ccgcctggag cggcgccttc
     7681 accaccggca tcgcggacgc aatggcgctc caggcgagaa tggccgtcga gatcgccagc
     7741 cgactggatc tcagcggcgc gaaggccgcc gggcagggcc gggcggcgct cctcctggtg
     7801 aagccgggcg cgtacgacgc ctatctgagg gccctggacg ccatggcctc gaacgacgcg
     7861 agccgggccg cgcagctgtt cgaggccgcg gcagacgagg accccggcct cgccgacgcg
     7921 caggccggcc ttgcgctggc gctcttcgtg gcgtcggcgt tcgaggggcg cggccacttc
     7981 ggcgacgtgc agacgcacgc gcggcatgcc gcggaggccg cctgcgcggc cgaccccgac
     8041 agcccgtccg cctggctgtc gatgggcctg acggcgccga ccatcagcgg cgctgtcgcg
     8101 cacctgaagc gcgccctcga actggacgcc tccgaagccc acgcgtatct cgcgctggcc
     8161 gggatcctcc ggcctgtcga tcccgatgcc gccctgcgct tcgcgcgccg cgcgggcgag
     8221 ctcgatccgg gtcagcccct cgccgtcctg atcgaagccg ccgcgcatct gtcggccggc
     8281 cggcccgccg aggcggtggc agcggccgaa cgcggccgct cgctgttctc cgagctgccg
     8341 tggtgggacg ccgtctgcct gcgcgcgaga ctgggccggg ggccgggacc ggcgggcgcg
     8401 cccccgccgg gaacccagcc ctggcgcgac gccggcgact tcccgccagg gacgatcctg
     8461 cgcgcagcgg agctggcggg ggccggccgg accggcgacg cgctgacgct gctcggcagc
     8521 ctcacgcggc tgcatcccgg ctcgtgcgaa gcccgcgcga tgactgccgc cgtgctcctt
     8581 cgcgccggca ggatgggaga tgcccagcgc ctcgccagcg cggtgtggtc gagggcggcg
     8641 gccgcgcccg acggcagcgg ctggtcgcgc tgcgcctaca tggcggcggc cgcgctgaac
     8701 gaacccgcga gggcggccgc ctggctcgcc cgcgccgcct cctccgagcg ggaactgcgc
     8761 ggctgggggg ccgtcagcgg cgtgctggac ggcctggccg ccacgcgcga cggcgtcttc
     8821 ccgtggtcga acgtctcggc cgcgccggcc gtcgccgaat cggccgcgtc gctgcgcgcc
     8881 gccatggatc ggacccgcgc cgagacggcc cgggtcctgc agggcagttg acaggaaggg
     8941 ctccatgctg atcgaatccc gggcggccgc gccgtttcac aagaacggct tcctcgtcgc
     9001 gtgcgacggc tcgcgcgagg ccgtgctgat cgacccgggc gacgaagtcg aggcgcttct
     9061 cgcgatcgcc ggcgcgcgcc gcctgtcgat cggcgcgatc ctgctcacgc acggccacgt
     9121 cgaccacgtg agcgggatcg cggcggccaa gcgggccacg ggggcccccg tgggcctcca
     9181 ccgcgaggac ctgttcctct acgagaacgc cgtcgagcag ggccggttct tcggctaccg
     9241 ggtcgagccg cccccgcctc cggacttctt cctcgccgaa ggcgcgcctc tctccttcgg
     9301 ctgctgcgcc gtggacgcgc accacacccc aggccacagc ccgggaggcg tctgcctgcg
     9361 cgtccgatgc cccgaggagc ccggcacgca cctcttcgtc ggcgacacgc tgtttgcggg
     9421 atcgatcggg aggaccgacc tgccgggcgg cgattacgag acgctgatgc gatcgctgcg
     9481 gacggtgctg ttcggcttcg gcgacgccgc cgtcgtgcat ccgggacacg ggccggacac
     9541 gacgatcggg cgcgagcgcg cgacgaaccc cttcctgcgc tccgcctagc ctacccgcag
     9601 ggctccagcg cgaactcgac gcggccgacg cgaaggacgc tgccgcgagc gagcgtcgcc
     9661 cggctgacgc gcttcccgtc gacccaggtg ccgttggtgc tgccgagatc gacgacttcg
     9721 acggcgccgt cagggcccac ttccacgcgg cagtgcaggc gcgagacgaa cggcacgtcg
     9781 aggcagaagt cggccgcccg ggcccggccg atcgtcttca cggcccctgg catcagccgg
     9841 aacgacaccg ggcgcgccgg gtcagtgcgc cgggcccgca gctcccacat gcagcgtgtc
     9901 cttcagccag ggcaccaggg cctccggcac cttggggctc acccgcctgt ctggcagcgt
     9961 ggcgtactcg tctggttccc ccgtcgccgc aggcacgagc agatgattga tcccctggat
    10021 ggtcacgagc gcggccggga ggggtctctt cctggcgcgg gccgcctcga tcagaagggc
    10081 gccgtgccgc gcgggaacct gcctgtcgat gtcgccgtga acgacgagca ccggctggcg
    10141 caccttctcc attaccctgg ccggatcgaa gagcagcagg ctgcggaacc acgccgtgtc
    10201 cgcctgctca cggtagcccg gcgggacggt gtcccagcct gtcccggtga tcaccgcgtg
    10261 gtgcagccgc tcctgcagct cgacgcgctg ctgccgctcg gcgtcgggca gggccatctt
    10321 ctcgatcagc cgcagctgct gctccatcac caggtcggat cccttcgagc ccggcgccgc
    10381 catgagcgcc agcgccgcca cgtcgtcttt ggcgccggcg gcggcgagca tcgccacgaa
    10441 cccgccttcg ccgtagccga cgagggcgat gcgcctggga tcgatgtcgc ggcgcttgcg
    10501 gaggtgcctg acgaccgcgc gcgcgtcttc cgcgtagtcg ccggccgtcg ccgactcgct
    10561 gcggccgccg ctctggccga ggccgcgctt gtcgtaccgg acgaccgcaa accccgcgtc
    10621 ggcgagcgcg ccggccagct gcgcgaagac ggggaccccc gcgacgttct cgtcgcgatc
    10681 ggcgaagccg ccgcccgaca cgaggaccac cgccggaagg gcgggcgcct ttccgcccgc
    10741 gcgcccggcc gccgccggca ccgatgccgg cttcgacagc gtgccggcca ggttgaaccc
    10801 gaaggccgcc accgtaatcg cttcgtcgtt cggccgcgcc agccgctcca cgcgcgtcga
    10861 gaccgccgcg acgtcctccc gggacacttc gagggcgttg gccggaacgc ggaagcgaag
    10921 cagccgcccc tcccggttcg cccagagctc ggcctcgacc ggccccgacg attcctcgat
    10981 cgtcagggtg taccgcctcg aatcgaccgc gccgtccatc gtctggaagc gttcgtcgcc
    11041 gacggccgtc acggcaatcg cgatctcgcc ctggccgacg atgtagccgc gcagagcggc
    11101 cggaacgggg gccgtcctca ggcggagggc aagcgcctcg aaggccgaga agaaggcctt
    11161 cggcggcagc accaccgggt ccgcggacac cgcgtccacc ttccaggagg actttccgcg
    11221 gcgcgtgatc tcgctcgtcg cgctgccgtt ggcgaaggtg gtcttcgtca cgctcggctc
    11281 gccctggagc gtcgcgctga tctcggcgcc gagcggacgc cagtcgcggt cgtagcggac
    11341 ctggaactcc ccggtccgga gatccaccgg gtcggcaagc cgccccgagc cgctgagcgt
    11401 ccatccgtcc gctgtggccc tcacttgcgc ccgctcgaag ccgagctggc gtccggccat
    11461 aaacacgacg aacctgacgg cggccggctc aggctgctgg gcctggcctt cggcggccgg
    11521 ccccgcggcc gccgcgatga gagcggccag cgccagggtt cgccggatca cccggggcgc
    11581 acgcctctgc cgccgggctg atcgccccgc gggcccggct gtacaatggt cgcgagccat
    11641 gacgtttctg agcataccag agccatgacc attccggacg actacacagc ggcgcgcctg
    11701 gccgcggccg tggcgggcct tggacaccgg gatcggatcg tcgttcgcgg cgcggatcgc
    11761 aagtcctttc tgcatgcggt tttgacgaac gacatcgccg gactggcttc cggcacgggc
    11821 tgctatgcgg ccttcctgac gccgcaaggc cggatggtcg ccgacatgaa cctgttcgat
    11881 ctcggcgacg cggcgctcat cgacacgccg gcgggcgccg ggcagcggct tctcgagcgg
    11941 ttggaccagc tgatcttcag cgaggacgtc cagctcggcg acctgagcga ggcgtgggga
    12001 tgcctcggcg tctatggacc gcgcgccgcc gaggccgccg gcgcggcgat cggcgcggac
    12061 ctcgcggcgt tcgcgccctt ccagaacgcc cgcctggcgt tccgcggaga cactctcgtg
    12121 gcggcccggc gcgacgggct gggattgacc ggcttccttc tcttcgccgc cgccgggcag
    12181 gtggaagccc tcgaagcggc gttggccgag gcgggtgcgg tgaggatgtc cgcggctacc
    12241 gccgaggtcc tgcgcgtgga ggccggcgag ccggcgtttc ccgccgacat ggacgaggcc
    12301 accattcccc tcgaggccgg catcgagggg cgggcggtga gcttcacgaa gggctgcttc
    12361 ccgggccagg aagttctcgt gcgcatccgc gaccgcggcc acgggagaat cgcccggaag
    12421 ctggtcggcg tggcgctgga cggcagttcc gttccgtctc ccgggagccc gatcctggcc
    12481 ggggacaaag aggcaggccg cgtgacgagc gccgtcatgt cgcctgcgct cgggcggccc
    12541 gtcgccctcg gctacgtgca tcgcgatctc gccgagcccg gcactgcggt tgctgtcgcg
    12601 agcggtgccg ggtcgattcc tggcaccgtc accgcgctgc cgttcgtgac cccctgaaga
    12661 gctgacagtc tcctgttccg ggagggtgaa actctccagg ttccggcctg accgccgacg
    12721 cgggaattgg agaccgtcgc cttttccggg atccatgtgg agatcggggc tccagcccgg
    12781 attccggttg gaggccgggc cttcagacgc tgcgcgagat gagcagtccc gcgagctccg
    12841 cgtcggccgg cgggagggga accgcgccca ggtcggcgcg cgccacccac cgcatctcct
    12901 gtcccatgac cggcctgggc tcgccgagca gcaggcagcg gaagaaccgg agttcgacgg
    12961 atcggtccgg gtagtcgtgg attgtcgcga ggatctcctc gccaacctcg atcgcggcgt
    13021 cgagctcctc gcggatttcc cgcgccaggc attcctcgag cgtttcgccc tcgtgcagct
    13081 tcccgccggg gaactcccag tggccggcaa ggtgcgtccc ctcgagacgc ctcgccagca
    13141 ggaacgctcc gccgcgctcg atgaccgccg ccgccaccac gacccgcttc atcgccgggt
    13201 cacgcctgcc gccttccccg aacgcccgcc ttcgccggac ggcctgacgg tcggtttcgg
    13261 cgcggcaggc cgagccccga gcccagagcc ccgggcccca agtctgaacg cctcgcagaa
    13321 ccccgccatc gggcactcgc cgcacttcgg gtttcgcgcc gcgcacacca gcgccccgaa
    13381 atccatcagc gcctggttga agtcgaacac ccgcttgcgc ggcatcatgg cctcggagag
    13441 cgcccacagg tgtttgcgca tccggtgcga gccggcctcg ccgcggccga cgacgacccg
    13501 gaacagcacg cgcgcgacgt tggtgtccag gatcggcgcc cgcttgccga aggcgaagct
    13561 cagcatcgcg cccgccgtgt agggcccgat gcccttgaac gagcgcagcg ccgcttcgtc
    13621 ggccggcagc gcgccgccga acctggagac cgcctcgcgc gcgatggcgt gcaggcgccg
    13681 cggcctgatg ttgtacccga gcgggcgcca ggtttcgaca gccccttctt ctggggcccc
    13741 ggcgagcgcc tcgagcgtcg ggtacttgcc gagccactcg tggtacttcg gcagcacgcg
    13801 atgcacctga gtctgctgca gcatgatctc cgacaccagg atgtggtacg ggtcggtcgt
    13861 tctgcgccat ggcagatcgc gcccgttgcg gtcgtaccag cgcagcaggg cccgtcgaaa
    13921 cgcgcggcgc cgggccggcg gcggcaagtg gagacggcct ggggccgggc ggcgggatgg
    13981 cgggttcagg gacatcagcc tcgcggacgc ggttcaggac acttataatc caacaggcat
    14041 ggagaagatc tacacgcgga ccggggacgg cggcgcgacg acgctgttcg acggcacgcg
    14101 gatcgcgaag tccgacccgc gcgtcgatgc ctacggggag gtggacgagg cgaatgccgc
    14161 cgtgggccag gcgcgcgcgg cgggcctgga cgaggacctg gatcggatgc tcgcgcagat
    14221 ccagcgggac ctgttcgccc tcggatcgcg cctggccgac ccggcggagc gcatcgccgg
    14281 gcgggtcacc aaggtcgcgc tcggccccga cgacgtcacg cgcctcgaag gctggatcga
    14341 cgcctgcgcg gaagagctgc agccgctccg gcgcttcatc ctgccaggcg gatcgcccgc
    14401 ggccgcgtcc ctccatctcg cccggaccgt gtgccggcgg gccgagcgcc ggatggtggc
    14461 cctgggcgtc ctggaagtcg agttcctggc ctacatgaac cgcctctccg acctgctctt
    14521 cgtcctcgcc cgcgtggtga accgccgcgc cggcgtcgcc gagtccgagt ggtgacggcg
    14581 gtcagtgtcc gactagcgcc tagcgcctag ccccgagcgc ctgattcaat gacatccatc
    14641 ctcgtccatt accaggagat cgccctgaag ggccggaacc ggccgtactt catcggccag
    14701 ctcgtccgca acgtccgcag gcagacggcg gacctcggcg tgaaggacgt gcgcgcgctg
    14761 atgggccgga ttgaaatcgt cctcgggccg tcggccggct acgaggccgt cagcgagcgg
    14821 ctccggcacg tcttcggcat cgcgaacttc tcgaaggccg ggcgcgcggc gcgggatctc
    14881 gagtcggtgg catcggccgt cctcgcggac ctggagggcc acaggccggt gacgttccgc
    14941 gtcagcgcca agcgcggcga caagacgttc cccatgacgt cgccccagct cgagcgcgag
    15001 ctcggcgcgc gcattgtcga gcgcttcggc tggcccgtga agctcgagcg gcccgagatg
    15061 gtcgtgacgg tggaggtgct gccgaaccgg atcttctact cgttcggcaa agagaaaggc
    15121 gcgggcggga tgccgaccgg cgtgagcggg cgcgtcgcgt gcctcatgtc cggcggcatc
    15181 gactcgcccg tcgcggcgta ccggctgatg aggcgaggct gctcggtggt cccggtgcac
    15241 ttccacagcc acccgttcgt ctcgcgcgcg tcgcaggaga aggtgcgcga gctgtgcgag
    15301 gtgctcacgc gctaccagat gcacacgacg ctgcggctcg tgcccttcgg tgagctccag
    15361 cgccaggtcg tcctgagcgt gccggcgccg ctccgggtcg tgatctacag gcgcctgatg
    15421 ctccgcatcg ccgagcgcat cgcgcgcgcc agccgcgcga aggcgctggt cacgggcgaa
    15481 gtcatcggcc aggtcgcctc gcagaccctc gagaacctca cggtcatcgc ggacgccacg
    15541 agcatgccga tcctgcggcc gctcgtcggg acggataagg aggagatcgt cgccgaggcg
    15601 atgaggatcg ggacctacga aatctcgatc gtcccggacc aggactgctg tcagctgttc
    15661 acgccgcgca gccccgagac gcacgcgcgg cggtggcagg tggaggaagc ggaggcaaag
    15721 ctgccgatcg aggacatggt gagggcggcc gtcgaggcga ccgcgaccga ggagttccgg
    15781 tttcccccgc ctacttcctg atgacctcgg agcccgatac cggccccagg caggtcgatc
    15841 cgctgtagtt gatccgcacg cggtcgagcc cctcgggcag cgccgtgccc accagcgaga
    15901 aggtgcatgc catgccggcc ccggcgacgg ttccgctcac cctgaggtcg gcggtcaggc
    15961 cgttcagctt gccctcggcc gatccttcga gcgtcaggcc gccgccgcag accgccttga
    16021 acgtccccga cgccgaggtg ccggtcagcg acgtgatcgt ccactcgagg ttcccgcagg
    16081 ccttcggatc gagcgaggac gccacggccg cggtggacga ggtccagctt ccggccgaga
    16141 gcaccgcgag cgcgggggtc ggagacgccg ggttctcgtg cctcgtttcc acgcacgctg
    16201 ccgcggttcc gattgcggcc gcgagggccg ccgccactgc cattcgtcgc atgcgcgtca
    16261 tgatgcgtct cctcgcctga tgctgtcaac tgcctcgcgg tgtcgggccc gcggccccgg
    16321 cgcgtcctgg cccggccgag agctgacggc gcgtccgcct gcctgtcttt cggcccgcgg
    16381 cactccggcc tccagactga ttccgaaggc acggatccgt aatcgccggg acggatggcc
    16441 ggcgttccgc catccgtccc gttcccgccg aagtccggcc tggacgcggc cccgccgccc
    16501 ggcggacagg tcccagctgc ccgtcctagc cgccccgaca actggttgcg cgagcaagaa
    16561 ccgtgctcgc cccttgccgc tcgcccttgt ccgaccagcc ccttgccttt cgcccctcgc
    16621 ccctgtccga ccagcgcctg gcgcctagcc cccagcgcct gtcataatag ggacttatgt
    16681 ccctgctcaa gatctcccgc atgggccatc cgatcctgcg gaagaagatg aaggcggtcg
    16741 ccccgtcgtc catcgccacg ccggccttcc agaagctcat cgatgacatg gcgctgacca
    16801 tgctcgagaa cgacggggcc gggctggcgg cgccgcaggt tcacgaggac tgccgtctct
    16861 tcgtcgcccg ggtgttcaac gaccgcgaag acgacgagaa cgcggagccg gcggagcccg
    16921 agatcctcgc gctcgtcaat ccggagatcg tccccgcgag ccgccagatc gaggaagact
    16981 gggagggctg cctgagcatt cccggcctgc gcggcctggt gccccgctac cgggacatca
    17041 gggtgaaggc gcacgaccgg aacggcgagc ggatcgatct gcaggcgtcc gggttcatgg
    17101 cgcgggtgat ccagcacgag acggatcatc tcgacggcat cctgtttctc gaccgcatga
    17161 agtcgatgca gagcctgagc ttcatcgagg agttcgcgaa gtaccacgcg aagcgggagc
    17221 gggaatagag ttcagaggcc ggaatccaga agccggaatg ctgaccgtgc gatcgagaag
    17281 tccgcgacaa ggcgccggag gctgcactcc gctgcgtcga aacgaccctg ctgacatgca
    17341 gctttctaat tccggattct ggcttctgga ttccgaacag ccgtgaagct gcagcccgtc
    17401 gccatcgtct tcgccacggt cttcatcgac ctgctggggt tcgggatcat catcccgctc
    17461 ctgccgttct acgccgagac gttcggggcc acggcgttca ccgtgggcat gctcgccacg
    17521 tcgttctcgt tgatgcagtt cctgttcgcg cctttctggg ggcggctttc ggaccgcgtc
    17581 ggccgccgcc cggtgatcct ggtcgggctc ctcggatcga gcctcgccta tctcgcgttc
    17641 gggctggcct cgacgctgac gacgctgttc gccgcgaggg tcttcgcggg cattg
//
LOCUS       DAOSIF010000010        17307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_3494_length_17307_cov_4.367320, whole genome shotgun sequence.
ACCESSION   DAOSIF010000010 DAOSIF010000000
VERSION     DAOSIF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3494_length_17307_cov_4.367320"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..3618)
                     /locus_tag="PLT35_00875"
     CDS             complement(79..3618)
                     /locus_tag="PLT35_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOG27805.1"
                     /translation="MTRGPGVYPGDPREDFAPDLRIDGTTYRNLARLRPAYHSSSYDY
                     NLTAQLVTDGIVETVLPRTVVVATWQQGLLGAGSPSLPGQLGQGPLGGFSRQQMVWPR
                     HKREWALDENWVTSYTLRGPRVWIQLELRGGAEPLAFDEVRAQATVAALSPGPENWTC
                     SVLGSDDGLAWVPLGERSGLTAAGGDIFAAVGLRSLSRHRFVRVVFDDPRAVSWEIRD
                     VGFRRGGVAVRPGGPHQFSSAWMSAGRGEEWVYVDLGARCVFDRVKLHWLARPAEAAL
                     QVSDDASSWRTVRALAGGGGLDEVAFDPAVRARYVRVSMTKPASPAGYVLTEMEVFGR
                     GGPVPVPKPLGPRKADGRLDLAGGRWRIQRDSLVGAGGPALATAGFDDASWVAATVPG
                     TALVSYLNAGALPDPNYGDNQLMISDAFFHADFWYRTEFDAPSPGAGRRRWLAFDGVN
                     LNADVFLNGAPLGRIEGGFMRARFDVTGKLRDAGNALAVRVEKTTAPGSVKEKTFESP
                     DKNGGALGADNPTYHASIGWDWIPTIRGRNTGIWNDVYLTDSGPVTIDHPSVTTVLPL
                     PDTSRADVRLTAELRNHADVPVSGVLRGRFGDAAFEAPVTLPAGGFEEVVFDPSNAPA
                     LRVSAPRLWWPNGYGDQPLYDVELAFTAGGVAVPSDTKRFKAGVRQFTASFDHGALRM
                     WINGRRFIPRGGNWGFPESMLRYRGREYDAAVRYHKHLNFNMIRNWVGQTGDDEFYDA
                     CDRHGIVVWQDFWLANPWDGPDPASHRVFMANAEDLVNRIRTHPSVGLYCGRNEGYPP
                     KPLDDAIRRLLAERHGDLPYLSSSADDYASGHGPYRAMPVNDYFLRRAAPKLHSEMGM
                     PNVMTIDSLRAMLPDASLWPLGRLYGLHDFSLEGAQGGASFLERIQRSYGAADNAADW
                     STLAQFVNYEGYRAMFEAQSRNRMGLLIWMSHPAWPSLVWQTYDYYLEPTAAYFGARK
                     GAEPLHIQWNPATEAVEVVNYSAGDAPGLTARAEVRGIDGSLIWEKSAALDSKEDSTL
                     SVIDMEYPLGTSPVHFIRLALARGSETLSENFYWRGLEDGNFLQPRDLPKAAIDAATT
                     VTGTPEGYRLDTELVNRSSAPAIMVRLKAVRASSGDRILPVLYSDNYVSLMPGERRTI
                     TAEVTRADARGERPRMVVEGFNIGAAGPERPRR"
     gene            4000..5220
                     /locus_tag="PLT35_00880"
     CDS             4000..5220
                     /locus_tag="PLT35_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27806.1"
                     /translation="MRRPNLPAVLLCACLAAAAPVLSARQDRQECTTAIFAAGATADG
                     RPMLWKNRDTDRLSNKVVLVDDKPYSYLGLVNADHADGRMVWAGANAAGFAVANSVAY
                     NLPHESGEEADLEGIIMADALRTCETVGEFERYLARRTGPDLGAQANFLAMDAKGGAA
                     IFETHNHGFRRLDAGASPARYLANTNFARTGVADQGAGYLRFDRETALLEAAEPRSFT
                     PAWVLEVAARDLGHALLAHPARSAWKKLPADRPTWVHTNHTIDRASTASAVVVHGVKP
                     GEPVRHTTVWVALGEPVCAIAVPVWVAAGLPPAPLRDGEHAPLAAESRRLKDLLRPLR
                     ARERQEYADLAKLDNAAGTGWLPALVATEQAIMAEAAALLAKNPTPAQLAAFQQAAAE
                     RALAVLQSIKAPAP"
     gene            5269..5898
                     /locus_tag="PLT35_00885"
     CDS             5269..5898
                     /locus_tag="PLT35_00885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG27807.1"
                     /translation="MGSRLERWVVGLYDVVFEPFLGPARREGLRLHEPPPGALVVDAG
                     CGTGSHLRLYRDRGRRLAGIDLSPAMARAAARKLGSAASIQVGDASRMPYRGGCVDLV
                     LLSMALHALPPALGGTVLAEAARVLKGNGRILVLDYHAGPGGSLRDRAVRAGVHAVER
                     MAGGEHFANYRRYMTGGGLPPLAAAAGLRIDAAKAARRGSLGLYLLAAR"
     gene            complement(5951..6373)
                     /gene="nikR"
                     /locus_tag="PLT35_00890"
     CDS             complement(5951..6373)
                     /gene="nikR"
                     /locus_tag="PLT35_00890"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOG27808.1"
                     /translation="MSTLARIGVAIDGGLLRQFDALAARKKYGSRSEAFRDLIRDALV
                     QEQWAAGAGPVVGTITLVYDHRVRLLGERLTGVQHDAHRDILSTLHVHLDHDHCLEVI
                     VVRGRAARVREVADQLISMKGVKHGRLTIASAGAGRAR"
     gene            6421..7476
                     /locus_tag="PLT35_00895"
     CDS             6421..7476
                     /locus_tag="PLT35_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG27809.1"
                     /translation="MSTDGPAPPRPPARAPGASAGAWWLGGLGAMLAAAAGFALLTGA
                     YRLAPSDVLALLLPGSGAGGADAPVAWAVIWNLRLPRILLAILVGAALASSGAVFQAS
                     FRNPLVEPYILGVSSGAAFGAALGLLLPRFPLSVQAAAFLGALAAVGLVHLLARSRGD
                     APIVTLVLSGVIVGAMFAAGVSMVKYLSDDAALRQLVFWLMGGFYFASWGDVTVAGPI
                     VAASAAVMIAGGWRLNVLSMGDHEAATLGVDPRRARPVLLGAATLATAVSVSVAGIVP
                     WIGLMTPHAIRLLIGPDNRLVVPGSALLGAVILLACDTAARSITQSEIPVGIITALAG
                     GPFLAVLVRQRTAGLFG"
     gene            7481..8272
                     /locus_tag="PLT35_00900"
     CDS             7481..8272
                     /locus_tag="PLT35_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG27810.1"
                     /translation="MLSVRNLRFSYGAAPVLRGVSFDAAAGRVCGLFGPNGCGKTTLF
                     RCCLGLLRGAADVLAVGGNRVDRLPPAGRARLMAYVPQEHKPPFPLLVRDVVMLGRTP
                     YLGLLGRASDADRRIVGETIDWIGLGDLAGRPYDRLSGGQRQLVLIARAVAQRTPVIL
                     LDEPASGLDFHNQVRIWGVLRSLAASGAAVLCCTHDPNHVAWFCDRVLVMQAGAIAAQ
                     GPPGEALSARTLELLYPGVCAPGVAGGTPVILPKAVTSRRGSPGP"
     gene            8269..9507
                     /locus_tag="PLT35_00905"
     CDS             8269..9507
                     /locus_tag="PLT35_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG27811.1"
                     /translation="MTRPRDVLVALAASGAIAAAGCRAPGREAAHGGAAEGAARPAIR
                     TVIDMRGAAVRVPARLTRVATINDGFVESVMSRLGTIRTLVAVGSSSQQRVWAYTYAA
                     DDGRTFSVADGMGTMRALHPWLAGLPCASYTSGDAVNYETIASARPEIVIVRVGDCTV
                     GPSGEAVSRLLEVFGAMGIPAVVLRSPADYRGRGLETLRAEIEVLGRVFGEEREAAAL
                     ADELGESEAMIRARVGGVPALERPRVLYLGLSAGASSSGGAAFAWGPGSVESWMIEEV
                     IGARNAYRGPGARVVLNAEQILALDPDVIFLPTSGGYHPPWELAEAPRFRELRRLRAV
                     RDRRVYPLPWTPMNCARRLEYPLDLLVMAKGAFPRRFADVAVHAWAEEFYRRTYRVDA
                     GQARMLLRAQWLDWTAEAGF"
     gene            9616..10506
                     /locus_tag="PLT35_00910"
     CDS             9616..10506
                     /locus_tag="PLT35_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG27812.1"
                     /translation="MLRLSLLLSLTASLAAQAPPAAGAASGPPPTQAAARPAAGAPLT
                     IMTFNVRYGTAEDGENHWRHRREQLFALLREQRADAVGLQEALRFQLDEIVASVPGYA
                     FVGVGRADGRQDGEYAAILYRTDRLTPRRTDTFWFSDTPGVVRSVSWGNRIERICTWA
                     YFEDREGPPFYLYNLHLDHQSQPSRERSVELLLKVVGARDPRAPAVVTGDFNAGEDNA
                     ATAAMRASFRDSFRVLHAEAAEVGTFSGFAPGRTGGDKIDFVFVEPGTEVLEAAIVRS
                     SRAGRYPSDHFPVTARIRLR"
     gene            10562..13336
                     /locus_tag="PLT35_00915"
     CDS             10562..13336
                     /locus_tag="PLT35_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG27813.1"
                     /translation="MKPITSRLVLPGLLVLAVTAAAVWSWGRAPRAITVALLEAPGRG
                     EVPAIGADAATLISGRRVTPAGRTIRTQSYNWGMAVAPDQSRVALIRPSAIEIVGLAP
                     DAPSVRLPPYGEKPAKEFGEGTYMGVAFSPDNRLLYFGSANAGEIKAMDVASSRIVWT
                     VSLDGGGYRDSFAGDFALGADGQRLYALDQFNYRLAIVDVKRRAVVRSVRVGRNPFSV
                     SLSPDGRHAWVTNVGMFEYPLLPGVTEANRATAGLTFPAYGVPSKEAEDGTVAEGLGV
                     PGLGSPNHPDAMSLVRVDLETGAVTARVKTGYLVGAERDGLRTIGGSSPGGVVAGRRR
                     VYVSNATNDTITVVDAASGAVAGQIALDVPGLERLRGVIPFGLALGPDERRLYVACAG
                     LNAVAVVDTEAQALEGYIPAGWFTSDVAVSPDGRTLYVSSAKGLGSGPNGGRGFVAPA
                     RGLHPAEIMQGTLQIVPVPAPGSLEPLTRQVLDNTLARREAVVPPGHPLYAALAGRAA
                     DGPIRHVVFVVKENRTFDQVFGQRRGVDGDPSLTTLGFGMPVKRKDGTLAVDGANVSP
                     NHQALADAYAISDNFYCDADQSNTGHRWVAGVYPNEWVEVNARSRIEERLFSPAPGRR
                     YVSGSSAVVNPEDYNEAGALWEHLDRHGVPFFNFGFGTEMPASIAQQAFKETGIRMAV
                     SFPLPKPLFDRTSRKYATFNMAIPDQYRMDMFEEELRDRWLSGKEAFPRLVTLVLPND
                     HLTEEHPADGYPFVESYMADNDLALGRLIHTLSRTPWWENMLVIVTEDDPQGGRDHVE
                     AHRSILMLIGPHVRRGYVSHTLADFGSIMRLIFTVLGLPPLNQFDAVAPLPLDLFAVG
                     RSDASPYTARPPDARIFNPDEAFKPFDRRFNWQRLAGSPRMDDPDDMLRPFREPGSAQ
                     AAVVLGGERR"
     gene            13449..13607
                     /locus_tag="PLT35_00920"
     CDS             13449..13607
                     /locus_tag="PLT35_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27814.1"
                     /translation="MATKRTTHRSVSGTKLYAVRDSKGRFKDIQTYKRAHAADLRKKS
                     AAEKKAAK"
     gene            complement(13626..14420)
                     /locus_tag="PLT35_00925"
     CDS             complement(13626..14420)
                     /locus_tag="PLT35_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG27815.1"
                     /translation="MSFEHVDFAELYRRDPGVISRAEHPPEFWDGRAAGFGERAARSE
                     YGRQFVERMDLDGCSTLLDVGCGPGTIALAVAGRLERVYGLDYSPRMLDAFSERAKAA
                     GHSNATPILRAWADDWSDVPVCDVVVASRATAVRDFEAAARKLDAVAARRVYLTYPAF
                     GTFAGDGIRRALGRPHHPLPDYLHIVGILHHLGMHPRLDYITEKGRFDGCEGFEAFVG
                     KVRGLTGDLSGAEADVVRQYYETHRGELAAERMRWAFISWDAAPRR"
     gene            14632..15843
                     /gene="speY"
                     /locus_tag="PLT35_00930"
     CDS             14632..15843
                     /gene="speY"
                     /locus_tag="PLT35_00930"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOG27816.1"
                     /translation="MTKKKTAHVRYRKDLSEMPEWMKKKALPQRQKYFSGRRVLPRNV
                     TGREKLADLVDDAFLAYNSGRLREGCQLFVEKMLEPDVTIGMSLSGALTPAGLGVSSI
                     VPLIRAGFVDWIVSTGAILYHDLHFALDYPIRMGSFRMDDTELRDNDIVRVYDVLMGY
                     SDCLMATDEILRAILLQPEFQREMGTAELHHLLGKYAAEWERKAGLKNVSLLAAAYRA
                     GVPCYTSSPGDSTIGMNVAGVELRGSKLRVNPSIDVNETTAFVLAAKRSGGKSGVVLW
                     GGGSPKNFMLQTEPQIQEVLRIKEFGQDYFLQVTDARPDTGGLSGATPSEAVSWGKVD
                     PARLPDAVVCYTDTTIAMPILTHYALARHKPRPLRRLYDARPAMVKALTREYFAHNKV
                     RMIDGSAPVLD"
     gene            15847..16989
                     /locus_tag="PLT35_00935"
     CDS             15847..16989
                     /locus_tag="PLT35_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOG27817.1"
                     /translation="MARLTAKQLQDLAWEHGTPLVVVNHDAIRENYATFKRLLPRVQG
                     YYAVKANPAPEIVRTLFKAGASFDVASLSEFMLVYDLIRHRPARVRQDFVWDKIVYAN
                     PVKAKETLESLDKYKPLVTFDNGTEVVKIREFAPRAGLLLRIRVPNTGSMVELSSKFG
                     CDPGEAVSLMEEARAAGLEVEGLSFHVGSQCTNFENYVQALNMSAAVMREAASRGFDL
                     RVLDIGGGFPVRYNRHVPPFSLLARRINAEIDRLFPGDVEIIAEPGRFFVATAITSIA
                     TVIGKAVRDGKTCYYIDDSVYHTYSGIIFDHCPYRIHAFKKGPAEVSAVFGRTCDGLD
                     TISQAEMLPDLQLDDLVYSLDIGAYSNASATWFNGFPPAKVVHVNE"
     gene            17073..>17307
                     /locus_tag="PLT35_00940"
     CDS             17073..>17307
                     /locus_tag="PLT35_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27818.1"
                     /translation="MTALSLALAAALAAHAPAQAPKADDYVGTWNVRITDAADTFVSG
                     GFQVDKAGDGLTGGLVWRSGSFTPAASVEVSDGA"
BASE COUNT         2335 a         6192 c         6357 g         2423 t
ORIGIN      
        1 cattgacaac gaccactgga agcggcccgt cgccgtcccc ggtgcggaag cggctcgggc
       61 gccggcgccg aacccgcgct agcgccgcgg gcgctccggg cccgccgcgc cgatgttgaa
      121 cccctcgacg accatgcgcg ggcgctctcc gcgggcgtcc gcgcgggtga cctcggccgt
      181 gatcgtgcgg cgctcgccgg gcatcagcga gacgtagttg tcgctgtaga ggaccggcag
      241 aatccggtcg ccggacgagg cgcgcaccgc cttcagcctg accatgatgg ccggcgcgct
      301 cgagcggttg accagttcgg tgtccagccg atacccctcg ggcgtgccgg tgaccgtggt
      361 cgccgcgtcg atcgcggcct tcggcagatc ccgcggctgc aggaagttcc cgtcttcgag
      421 cccacgccag tagaagttct ccgacagcgt ctcgctgccg cgcgcgagcg ccaggcggat
      481 gaagtggacc gggctcgtcc cgagcgggta ctccatgtcg atgaccgaca gcgtggaatc
      541 ctccttgctg tcgagcgccg ccgacttctc ccagatcagg ctgccgtcga tgccgcgcac
      601 ctccgcgcgc gcggtcaggc ccggcgcgtc tccggcgcta tagttcacca cctcgacggc
      661 ctcggtcgcc gggttccact ggatgtggag cggctcggcc cccttgcgcg cgccgaagta
      721 ggcggccgtg ggctcgaggt agtagtcgta ggtctgccag acgagcgagg gccacgcggg
      781 gtggctcatc cagatgagca ggcccatgcg gttgcggctc tgcgcctcga acatggcgcg
      841 gtagccctcg tagttgacga actgcgccag cgtggaccag tccgcggcgt tgtcggcggc
      901 gccgtagctc cgctggatcc gctcgaggaa cgacgcgccg ccctgggcgc cctcgagcga
      961 gaagtcgtgc agcccgtaca gccggccgag cggccacagc gacgcgtcgg gcagcatcgc
     1021 cctcaggctg tcgatggtca tgacgttcgg catgcccatc tcgctgtgca gcttgggcgc
     1081 ggcccgccgc aggaagtagt cgttgaccgg catcgcccgg tacgggccgt gcccgctcgc
     1141 gtagtcgtcg gccgagctgg agaggtaggg caggtccccg tgccgctccg ccaggagacg
     1201 ccggatggcg tcgtcgaggg gcttcggcgg gtagccctcg ttgcgcccgc agtagaggcc
     1261 gacggacggg tgcgtgcgga tccggttgac gaggtcctcc gcattcgcca tgaaaacgcg
     1321 gtgcgacgcc gggtcggggc cgtcccaggg attggccagc cagaagtcct gccagacgac
     1381 gatgccgtgg cgatcgcagg cgtcgtagaa ctcgtcgtcg cccgtctggc cgacccagtt
     1441 gcggatcatg ttgaagttca ggtgcttgtg gtagcggacg gccgcgtcgt actcgcgccc
     1501 gcggtaccgc agcatcgact cgggaaagcc ccagttgccg ccgcgcggga tgaagcggcg
     1561 gccgttgatc cacatccgca gcgccccgtg atcgaagctc gcggtgaact ggcggacgcc
     1621 ggccttgaac cgcttcgtgt ccgacggcac ggcgacgccg cccgcggtga aggcgagctc
     1681 gacgtcgtac agcggctgat cgccgtaccc gttcggccac cagagcctcg gcgccgacac
     1741 gcgcagcgcc ggggcgttcg acggatcgaa gacgacctcc tcgaagccgc cggccggcag
     1801 cgtgaccggg gcctcgaagg cggcgtcgcc gaaacgcccg cggaggacgc ccgatacggg
     1861 aacatcggcg tggttgcgca gttcggccgt caggcgcacg tcggcgcgcg aggtgtcggg
     1921 caggggcagc acggtcgtga ccgaggggtg gtcgatggtg acggggccgc tgtcggtcag
     1981 gtagacgtcg ttccagatgc ccgtgttgcg gccgcggatg gtcgggatcc agtcccagcc
     2041 gatcgacgcg tggtaggtcg ggttgtccgc gccgagcgcc ccgccgttct tgtccgggct
     2101 ctcgaacgtc ttctccttca cgctgccggg cgcggtggtc ttctcgacgc gaacggcgag
     2161 ggcgttgccg gcgtcacgca gcttcccggt gacgtcgaac cgcgcgcgca tgaacccgcc
     2221 ctcgatgcgg ccgagcggcg cgccgttgag gaagacgtcg gcgttcaggt tgacgccgtc
     2281 gaaggcgagc cagcgccgcc gcccggcgcc gggcgaaggc gcgtcgaact ccgtgcggta
     2341 ccagaagtcc gcatggaaga acgcgtccga gatcatcagc tggttgtcgc cgtagttcgg
     2401 gtccggcagc gcgcccgcgt tcaggtagct gacgagcgcc gtgcccggca ccgtcgcggc
     2461 cacccacgag gcgtcgtcga accccgccgt ggcgagcgcg gggcccccgg cccccacgag
     2521 cgagtcccgc tggatgcgcc accggccgcc ggcaaggtcc agccgcccgt cggccttccg
     2581 ggggccgagc ggcttgggca ccggcaccgg gccgccccgc ccgaacacct ccatctccgt
     2641 cagcacgtag cctgccgggc tggcgggctt cgtcatcgac acgcggacgt agcgggcgcg
     2701 caccgccgga tcgaacgcca cctcgtccag gccgcccccg cccgcaagcg cgcggaccgt
     2761 gcgccacgag gaggcatcgt ccgagacctg gagcgcggcc tcggcaggcc gggcgagcca
     2821 gtgcagcttc acgcggtcga acacgcaccg ggcgccaaga tcgacgtaga cccactcctc
     2881 cccgcgcccc gcgctcatcc acgcgctgga gaactggtgc ggcccgccgg ggcgcacggc
     2941 gacgccgccg cggcggaagc cgacatcgcg gatctcccac gacaccgcgc gcggatcgtc
     3001 gaacacgacg cggacgaacc ggtggcggga cagcgaccgc aggccgacag ccgcgaagat
     3061 gtcgccgccg gccgccgtca ggccggagcg ctctcccagg ggaacccacg ccaggccgtc
     3121 gtcggagccg agcaccgagc aggtccagtt ctccgggccc ggactcagcg cagccaccgt
     3181 ggcctgcgcg cggacctcgt cgaaggcgag cggctcggcg ccgccccgca gctcgagctg
     3241 gatccacacg cgcgggccgc ggagcgtgta ggaagtgacc cagttctcgt cgagggccca
     3301 ctcgcgcttg tgcctgggcc agaccatctg ctgccgtgag aagccgccga gcggcccctg
     3361 cccgagctgg ccgggcaggc tcggcgaccc ggcgccgagc aggccctgct gccacgtggc
     3421 gaccacgacg gtgcggggca ggaccgtctc gacaatgccg tcggtgacga gctgcgccgt
     3481 caggttgtag tcgtaggagc tggagtgata cgcggggcgg aggcgcgcca ggttccggta
     3541 cgtcgtgccg tcgatgcgca gatcgggcgc gaagtcctcg cgcggatcgc cgggatagac
     3601 gccggggccc cgcgtcacga ggccatccga cgaggccgtg ccgggattcg gcgcccgcgc
     3661 gggactcgag ggcggtcggc gcggcgtcat ggcctgagcg ccggccagct gcgggccggc
     3721 gaagaggaga gcggcggcta gtatcgtgaa tcggtgcatc ggttcccccg ccggcggcgc
     3781 acggggcccg cgtgagcgcc ggcgtgccgg tccgtctgct gaacgcctgt ggacagcgga
     3841 ttctcctgcg gcctgatgat acgctccggc cgtcgcccgg ggtcaagatc gccgccgccg
     3901 aggacccccg ctccccgggg tcgtccgcgc gcccagcccg acgattcgcc gggcggcccg
     3961 gttgccaggc atgcccgggg tggaacacaa tccgtcgcca tgagacgccc gaaccttcct
     4021 gccgtcctgc tttgcgcgtg cctggccgct gccgcgccgg ttctctccgc ccgccaggac
     4081 cgccaggagt gcaccacggc catcttcgca gccggggcga cggccgacgg gcggcccatg
     4141 ctctggaaga accgggacac ggaccggctg tccaacaagg tggtgctcgt cgacgacaag
     4201 ccgtacagct acctggggct cgtcaatgcc gatcacgccg acggccggat ggtgtgggcg
     4261 ggcgcgaacg ccgcggggtt cgccgtggcc aactccgtcg cgtacaacct gccgcacgag
     4321 tcgggcgagg aagccgatct cgaggggatc atcatggccg acgcgctgcg cacgtgcgag
     4381 accgtcggcg agttcgagcg gtacctggcg cggcgcaccg gccccgacct cggcgcgcag
     4441 gcgaacttcc tcgcgatgga cgcgaagggc ggcgcggcga tcttcgagac gcacaaccac
     4501 ggcttcaggc ggctcgatgc cggggcgagc ccggcgcggt acctggccaa cacgaacttc
     4561 gcgaggaccg gggtggcgga ccagggcgcg ggctacctgc gcttcgaccg cgagacggcg
     4621 ctgctcgaag cggccgagcc gcggagcttc actcccgcgt gggtgctgga agtggcggcg
     4681 cgcgacctgg gccacgcgct gctcgcgcat cccgcccgca gcgcgtggaa gaagctgcct
     4741 gccgatcggc cgacgtgggt gcacacgaac cacacgatcg accgggccag cacggcgagc
     4801 gcggtggtcg tgcacggcgt gaagccgggc gagcccgtgc ggcacacgac ggtgtgggtc
     4861 gcgctcggcg agccggtgtg cgcgattgcg gtgccggtgt gggtggccgc gggcctgccg
     4921 cccgcgccct tgcgcgacgg cgaacacgca cccctcgcgg cggagagccg gaggctgaag
     4981 gacctgctgc ggccgctccg ggcccgggag cgccaggagt acgccgacct cgcgaagctg
     5041 gacaacgcgg caggcacggg atggctgcca gcgctggtcg ccaccgagca ggcgatcatg
     5101 gcagaggcag cggcgctgct cgcgaagaac ccgacgccgg cccagctcgc ggccttccag
     5161 caggccgcgg ccgaacgcgc gctcgccgtc ctccagtcga tcaaggcgcc ggcgccctag
     5221 cccgccgtcg cgcgccacgc gagggggccc ctcgcgtagc atgagtggat gggatcacga
     5281 ctcgagcggt gggtcgtcgg gctgtacgac gtcgtgttcg agccgttcct ggggcccgcg
     5341 cgacgcgagg gcctgcggct ccatgagccg ccgccgggcg cgctcgtggt cgatgcggga
     5401 tgcggcaccg gcagccacct gcggctctac cgggatcgcg ggcgcagact cgccggcatc
     5461 gatctgtcgc cggccatggc gcgcgcggcc gcccggaagc tggggagcgc ggcctcgata
     5521 caggtcggcg acgcgtcccg gatgccgtac cgcggcggct gcgtcgatct cgtcctgctc
     5581 tcgatggcgc tgcacgccct gccgcccgcc ctgggcggca cagtcctggc ggaggcggcc
     5641 cgcgtgctca aggggaacgg gcgcatcctc gtgctcgatt accatgccgg gcctggcgga
     5701 tcgctgcggg atcgcgccgt tcgggcgggc gtccatgccg tcgagcggat ggcgggcggc
     5761 gagcacttcg cgaactaccg ccggtacatg acgggcggcg gcctgcctcc gctggcggcc
     5821 gccgcggggt tgcgcatcga cgccgcgaaa gccgcacgac gcggcagcct cggcctctat
     5881 ctgctcgccg cgcgctgacc tgctccgcct cgaccgcgga cgtccgggcg gcagcttcgc
     5941 gccgctcgcc tcatcgggcg cggccggcgc cggccgacgc gatcgtcagc cgcccgtgct
     6001 tcacgccctt catgctgatc agctggtcgg ccacctcccg gacgcgcgcc gccctgccgc
     6061 ggacgacgat gacctccagg cagtggtcgt gatccaggtg cacgtggagc gtcgagagga
     6121 tgtcgcggtg cgcgtcgtgc tggacgccgg tcagccgctc gccgagcagc cggacgcggt
     6181 ggtcgtagac gagcgtgatg gtgccgacga cggggcccgc cccggcggcc cactgctcct
     6241 ggacgagcgc gtcgcggatg aggtcgcgga aggcctcgga gcggctgccg tacttcttcc
     6301 tcgccgcgag cgcatcgaac tggcggagga ggccgccgtc gatggcgacg ccgatgcgcg
     6361 ccagggtgct catggctggc gagcgtaaca cgattgggag attcgtggta cgcttccccg
     6421 gtgtccacgg atggccccgc gccgcctcgg ccgcccgcgc gcgcgcccgg cgcgtccgcc
     6481 ggcgcctggt ggctcggcgg gctcggcgcg atgctcgcgg cggcagcggg gttcgccctc
     6541 ctcaccggcg cctaccgcct ggccccgtcc gacgtcctcg ccctcctgct gccgggctcc
     6601 ggcgcgggcg gcgcggacgc gcccgtcgcc tgggcggtga tctggaacct caggctgccg
     6661 cggatcctcc tggcgatcct cgtcggcgcg gcgctggctt cgtcaggggc cgtcttccag
     6721 gcgtcgttcc gcaatcccct cgtcgagccc tacattctcg gcgtgtcctc cggggccgcc
     6781 ttcggcgcgg cgctcgggct gctgctgccg cgcttccccc tctcggtgca ggccgccgcg
     6841 tttctcggcg cgctggccgc cgtcgggctc gtccacctgc tcgcccgctc ccgcggcgac
     6901 gctcccatcg tcacgctggt cctgtccggc gtcatcgtcg gcgcgatgtt cgcggcgggc
     6961 gtctcgatgg tcaagtacct ctctgacgat gcggcgctgc ggcagctggt gttctggctc
     7021 atgggcggct tctacttcgc gagctggggc gacgtgacgg tggccggccc catcgtcgcg
     7081 gcgtcggcgg ccgtcatgat cgccggcggc tggaggctca acgtcctgtc gatgggcgac
     7141 cacgaggcgg ccacgctcgg cgtggacccg cggcgcgcga ggcccgtcct gctcggggcg
     7201 gcaactctcg cgacggcggt ttcggtgtcc gttgccggca tcgtgccctg gatcggcctg
     7261 atgacgcctc acgccatccg cctgctgatc gggcccgaca accggctggt cgtgcccggg
     7321 tcggccctgc tcggggccgt catcctcctc gcctgcgaca ccgcggcgcg ctcgatcacg
     7381 cagtccgaga ttcccgtcgg catcatcacg gcgctcgccg gcgggccgtt cctcgccgtt
     7441 ctcgtccggc agcggaccgc cgggctgttc gggtagcgcg gtgctgtcgg tccgcaacct
     7501 ccgcttctcg tacggcgcgg cacccgtgct gcgcggcgtg tcgttcgacg ccgcggcggg
     7561 acgggtgtgc ggcctgttcg gcccgaacgg gtgcggaaag accacgctgt tcaggtgctg
     7621 cctgggcctg ctccgcggcg ctgccgacgt gctcgccgtc ggcgggaacc gcgtcgaccg
     7681 cctgccgccg gccggccggg cgcggctgat ggcctacgtt ccgcaggagc acaagccgcc
     7741 gttcccgctg ctcgtgcgcg acgtcgtcat gctcgggcgc acgccctacc tcggcctgct
     7801 cggccgggcg agtgacgccg accgccggat cgtcggggag acgatcgact ggatcgggct
     7861 tggcgacctg gccgggcgcc cgtacgaccg gttgtccggc ggccagcgcc agctggtcct
     7921 gatcgcgcgg gccgtggcgc agcgcacgcc ggtgatcctg ctcgacgagc cggcgtcggg
     7981 cctcgacttc cacaaccagg tgcgcatctg gggcgtgctg cggagcctgg ccgcctcggg
     8041 cgccgccgtg ctgtgctgca cccacgaccc gaaccacgtc gcctggttct gcgaccgcgt
     8101 cctcgtgatg caggccggcg ccatcgccgc ccagggcccg cccggcgagg cgctgagcgc
     8161 gcggacgttg gagctcctgt atccgggcgt gtgcgcgccg ggcgtcgccg gcggcacgcc
     8221 ggtcatcctg ccgaaggccg tcacgagccg gcgagggagc ccggggccgt gacccgcccg
     8281 cgcgatgtcc tggtcgcgct cgccgcctcc ggcgccatag cggccgccgg ctgccgcgcc
     8341 ccggggcgcg aggctgcgca cgggggcgcg gcggaggggg ccgcccgccc ggcgatccgc
     8401 acggtgatcg acatgcgggg cgccgcggtg agggtgccgg cgcggctgac gcgcgtggcc
     8461 acgatcaacg acggcttcgt cgagtcggtc atgtcgcggc tcggcacgat ccggacgctc
     8521 gtggccgtgg gatcgtccag ccagcagcgg gtctgggcct acacgtacgc ggccgacgac
     8581 gggcggacct tctcggtggc ggacgggatg ggcaccatgc gggccctcca cccctggctc
     8641 gccgggcttc cgtgcgcgag ctacacgagc ggcgacgccg tgaactacga gaccatcgcc
     8701 tcggcgaggc cggagatcgt gatcgtgcgg gtcggggact gcaccgtcgg gccgtcgggc
     8761 gaagccgtgt cgcgcctgct ggaggtgttc ggcgcgatgg gcattccggc cgtcgtcctg
     8821 cgctcgccgg ccgactaccg cgggcggggc ctcgagacgc tccgcgccga gatcgaagtc
     8881 ctcggccggg tcttcggaga ggagcgcgag gccgccgcgc tcgccgacga gctgggcgag
     8941 tccgaggcga tgatccgggc gcgcgtgggc ggtgtcccgg cgctcgaacg gccgcgggtg
     9001 ctctacctgg ggctttcggc cggcgcgtcc tcgtcggggg gcgccgcgtt cgcctggggc
     9061 cccgggagcg tcgagtcgtg gatgatcgag gaggtgatcg gcgcgcggaa cgcgtatcgg
     9121 gggccgggcg cgcgcgtcgt gctgaacgct gagcagatcc tggcgctgga tcccgacgtc
     9181 atcttcctgc ccacgtccgg cgggtaccat ccgccgtggg agctggccga ggcgccgcgc
     9241 tttcgcgagc tgaggcggct gcgcgcggtg cgagaccgcc gcgtgtatcc gctgccctgg
     9301 actcccatga actgcgcgcg ccgcctcgag taccccctgg acctgctggt gatggcgaaa
     9361 ggcgcgttcc cccgccgctt cgccgacgtg gccgtccatg cgtgggccga ggagttctac
     9421 cggcgcacct accgcgtcga cgcgggccag gcgcggatgc tcctccgcgc gcagtggctc
     9481 gactggaccg cggaggcggg cttctagccg cgcccaatcg cgccgcgcca ggcctccggc
     9541 tcggctcggc gcgcctcctg tctgcgggag atcggtgtat gatggcgacc ggcctgccct
     9601 ctccgaggac gtgcgatgct gcgcctgtcc ctgctcctgt ccctcactgc gtcgttggcc
     9661 gcccaggcgc cgccggcagc gggcgccgcg agcggaccgc cgccgaccca ggcggcggcc
     9721 cgtccggcgg ccggcgcgcc gctgacgatc atgacgttca acgtccgcta cgggaccgcc
     9781 gaagacggcg agaaccactg gaggcaccgg cgcgagcagc tcttcgcgct gctccgcgag
     9841 cagcgggcgg atgccgtcgg cctccaggag gcgctgcggt tccagctcga cgagatcgtc
     9901 gcctccgtgc cggggtacgc gttcgtgggc gtgggcaggg ccgacggccg gcaggacggc
     9961 gagtacgccg ccatcctgta ccggaccgac aggctgacgc cgcggcgcac cgacacgttc
    10021 tggttctcgg acacgccggg cgtggtgcgg tcggtgtcgt ggggcaaccg gatcgagcgg
    10081 atctgcacgt gggcgtactt cgaggaccgc gagggcccgc cgttctacct ctacaacctg
    10141 cacctggacc accagtcgca gccgtcgcgc gagcgcagcg tcgagctgct attgaaggtc
    10201 gttggcgccc gcgacccgag ggcgccggcc gtcgtgaccg gggatttcaa cgccggcgag
    10261 gacaacgccg ccacggcggc catgcgcgcg tcgttccgcg acagcttccg cgtgctgcac
    10321 gccgaagccg ccgaagtcgg gacgttctcc ggcttcgcgc cgggccggac cggcggcgac
    10381 aagatcgatt tcgtgttcgt cgagccgggc accgaggtgc tcgaggcggc catcgtgcgc
    10441 tcgtcgcgcg ctgggcgcta tccgtcggac cacttcccgg tgacggcccg catccggctg
    10501 cggtaacggc cgggcagggc cgcgccggcc gcgcacgccg tcaatcagtt cgggagcggc
    10561 catgaaaccc atcacgtcgc ggctcgtcct acccgggctt ctcgttctcg cggtgaccgc
    10621 cgcggccgtc tggtcgtggg gacgggcgcc gcgcgcgatc accgtcgccc tgctggaagc
    10681 cccgggacgg ggcgaggtgc cggccatcgg cgccgacgcc gccacgctca tcagcggccg
    10741 gcgggtgacg ccggccggcc ggacgatccg cacgcagtcc tacaactggg gcatggccgt
    10801 cgcgcccgac cagtcgcgcg tcgcgctcat ccggccgtcg gccatcgaga tcgtgggcct
    10861 ggcgccggac gcaccgagcg tgcggctccc gccgtacggc gagaagcccg cgaaggagtt
    10921 cggcgagggc acctacatgg gcgtggcgtt ctcgccggac aaccggctgc tgtacttcgg
    10981 cagcgccaac gccggcgaga tcaaggcgat ggacgtcgcc tcgtccagga tcgtgtggac
    11041 ggtgtccctg gacggcggcg ggtaccgtga cagtttcgcg ggcgacttcg ccctcggcgc
    11101 cgacggccag cggctctacg ccctggatca gttcaactac cggctggcga tcgtggacgt
    11161 gaagcggcgg gccgtcgtga ggtccgtgcg tgtcggccgc aacccgttct cggtctcgct
    11221 gtcgcccgac gggcggcacg cgtgggtgac caacgtcggc atgttcgagt acccgctgct
    11281 gccgggcgtc accgaggcca accgggcgac ggcggggctg acctttcccg cctacggggt
    11341 gccgtcgaag gaggccgagg acggcaccgt cgccgaaggc ctgggcgtgc cgggcctggg
    11401 gagcccgaac catcccgacg cgatgtcgct cgtgcgggtg gatctcgaaa cgggggccgt
    11461 gaccgcgcgc gtgaaaacgg ggtacctcgt cggcgccgag cgcgacggcc ttcggaccat
    11521 cggcggctcc agccccggag gggtcgtggc cggccgccgg cgcgtctacg tgtcgaacgc
    11581 cacgaacgac accatcaccg tcgtcgacgc ggcgagcggc gcggtggccg gccagatcgc
    11641 cctcgacgtg cccgggctcg agcgcctccg cggcgtcatt cccttcggcc tcgccctggg
    11701 cccggacgag cggcggctgt acgtcgcgtg cgcgggcctg aacgccgtgg ccgtcgtgga
    11761 cacggaggcg caggccctcg agggctacat cccggccggg tggttcacgt cggatgtcgc
    11821 ggtctcgccc gacggccgga cgctctacgt gtcgagcgcc aagggcctgg gctccgggcc
    11881 gaacggcggg cggggcttcg tggccccggc gcgcgggctg catccggccg agatcatgca
    11941 gggcacgctg cagatcgtcc cggttccggc cccaggcagc ctcgagccgc tgacccggca
    12001 ggttctcgac aacaccctcg cgcgccgcga ggccgtcgtc ccgccaggcc accccctgta
    12061 tgccgcgctc gccgggcgcg cggcggatgg cccgatacgc cacgtcgtgt tcgtcgtgaa
    12121 ggagaaccgc acgttcgacc aggtcttcgg gcagcgcagg ggcgtggacg gcgatccctc
    12181 gctgacgacg ctcggcttcg gcatgccggt gaagcgcaag gacggcaccc tcgcggtgga
    12241 cggggcgaac gtgtcgccca accaccaggc gctggcggac gcgtacgcca tcagcgacaa
    12301 cttctactgc gacgcggacc agtcgaacac cggccaccgc tgggtcgccg gcgtctaccc
    12361 gaacgagtgg gtcgaggtga acgcccggtc ccgcatcgag gagcggctgt tcagcccggc
    12421 gccgggccgc cgctacgtgt ccgggagcag cgcggtcgtc aaccccgagg actacaacga
    12481 ggccggcgcg ctctgggagc acctcgaccg gcacggcgtg ccgttcttca acttcggctt
    12541 cggcacggag atgcccgcca gcatcgccca gcaggcgttc aaggagaccg gcatccgcat
    12601 ggcggtcagc ttcccgctgc ccaagccgct cttcgaccgc acgtcccgga agtacgccac
    12661 cttcaacatg gccattcccg accagtaccg gatggacatg ttcgaggagg agctgcgcga
    12721 ccgctggctg agcgggaagg aggcgttccc tcggctcgtg accctcgtgc tgccgaacga
    12781 tcacctcacc gaggagcacc ccgccgacgg gtacccgttt gtcgagtcct acatggccga
    12841 caacgacctg gcgctgggac ggctcatcca cacgctgtcg cgcacaccgt ggtgggagaa
    12901 catgctggtg atcgtcacgg aggacgatcc ccagggcggc cgcgaccacg tggaggcgca
    12961 ccggtcgatc ctgatgctca tcgggcccca cgtgaggcgc gggtacgtct cgcacacgct
    13021 ggccgacttc ggctcgatca tgcggctgat cttcacggtg ctcggcctgc cgccgctgaa
    13081 ccagttcgac gccgtggcgc cgctgccgct ggacctgttc gccgtcgggc gctcggacgc
    13141 ctcgccctac accgcgcgcc cgccggacgc gcgcattttc aatcccgacg aggccttcaa
    13201 gccgttcgat cggcggttca actggcagcg cctcgccggg tctccccgga tggacgatcc
    13261 ggacgacatg ctccggccgt tccgcgagcc cgggtcggcg caggcggccg tggtgctcgg
    13321 cggcgagcgg cgctgagaga gcgcgcaccg ggcgcgcagg cggggtgttg ccgcgtccgg
    13381 gcggcggcac gataatgggc gcggtccgtc ggggaggccg acgtcactcg gccgggaggc
    13441 ggtgtctgat ggcgacgaag cgcacgaccc accgcagcgt gtcgggaacg aagctgtacg
    13501 ccgtccgcga cagcaagggc cgcttcaagg acatccagac ctacaagcgc gcgcacgcgg
    13561 cggatctgag aaagaagagc gcggcggaga agaaagccgc gaagtagcgc cgggcccgcc
    13621 gccgctcagc ggcgcggcgc ggcgtcccag gagatgaacg cccaccgcat cctctcggcg
    13681 gcgagctcgc cccggtgggt ctcgtagtac tggcgcacga cgtcggcctc ggcgccgctc
    13741 aggtcgccgg tcagcccgcg gaccttcccg acgaacgcct cgaacccctc gcagccgtcg
    13801 aaccggccct tctcggtgat gtagtcgagc cgcgggtgca tgccgaggtg atgcaggatg
    13861 ccgacgatgt ggaggtagtc gggcagcggg tggtgcggcc ggccgagcgc ccgccggatg
    13921 ccgtcgccgg cgaacgtccc gaaggcggga tacgtcaggt acacgcggcg cgccgccacg
    13981 gcgtcgagct tcctggcggc cgcctcgaag tcgcgcacgg ccgtcgcgcg cgaggcgacc
    14041 acgacgtcgc acaccggcac gtccgaccag tcgtccgccc acgcgcggag gattggggtg
    14101 gcgttcgagt gcccggccgc cttcgcgcgc tcggagaagg cgtccagcat gcgcgggctg
    14161 taatcgagcc cgtacacgcg ctcgaggcgc cccgcgacgg cgagagcgat cgtgccgggg
    14221 ccgcacccga catcgagcag cgtcgagcac ccgtcgagat ccatgcgctc gacgaactgg
    14281 cgcccgtatt ccgaccgggc ggcgcgctcg ccgaagcccg ccgcgcggcc gtcccagaac
    14341 tcgggcgggt gctcggcccg gctgatgacg cccgggtccc ttcggtacaa ctcggcgaaa
    14401 tcgacgtgct cgaagctcat gtcgggccgc gtgcgcccgg ccgcgccgca accggcgcgt
    14461 ggcgccaccc gtgcgagcgt agcacgaggc ggcgtccggc cgccgcccgg gcggacctat
    14521 aatgcaggga ccgaccggcg cgtgcgcggc ggttcgaggc cgccctgtgc ggcggcggcg
    14581 cgcgcggggc cgccgggacg cgcgccggca agacggggag catctgcagc catgacgaag
    14641 aagaagaccg cccacgtccg ctaccgcaag gacctgtcgg agatgcccga gtggatgaag
    14701 aagaaggccc tgccgcagcg gcagaagtac ttctccggcc ggcgcgtcct gccccgcaac
    14761 gtgaccggca gggagaagct cgccgacctg gtggacgacg cgttcctcgc gtacaactcg
    14821 gggcggctcc gcgagggctg tcagctgttt gtcgagaaga tgctcgagcc cgacgtgacg
    14881 atcggcatga gcctgtccgg cgcgctcact cccgccggcc tgggcgtgtc gtcgatcgtg
    14941 ccgctcatcc gggcgggctt tgtggactgg atcgtctcca cgggcgccat cctgtaccac
    15001 gacctgcatt tcgccctgga ctacccgatc cgcatgggca gcttcaggat ggacgacacc
    15061 gagctgcgcg acaacgacat cgtccgcgtg tacgacgtgc tgatggggta cagcgactgc
    15121 ctgatggcga ccgacgagat cctgcgggcc atcctcctgc agcccgagtt ccagcgcgag
    15181 atggggacgg ccgagctgca ccacctgctc gggaagtacg ccgccgagtg ggagcggaag
    15241 gcggggctga agaacgtctc gctgctcgcg gcggcgtacc gggccggcgt gccgtgctac
    15301 acctcgtctc ccggcgactc gaccatcggc atgaacgtgg ctggcgtcga gctcaggggc
    15361 agcaagctgc gagtgaatcc gtccatcgac gtcaacgaga cgaccgcgtt cgtgctcgcg
    15421 gccaagcggt ccggcggcaa gagcggcgtg gtgctgtggg gcggcgggag cccgaagaac
    15481 ttcatgctgc agaccgagcc ccagatccag gaggtcctgc gcatcaagga attcggccag
    15541 gactacttcc tgcaggtgac cgacgcgcgg ccggacaccg gcggcctgag cggcgccacg
    15601 ccgagcgagg cggtgagctg gggcaaggtg gaccccgccc ggctccccga cgccgtggtc
    15661 tgctacaccg acacgaccat cgcgatgccc atcctgacgc actacgcgct ggcgcggcac
    15721 aagccgcggc cgctgcgccg tctgtacgac gcgcggccag cgatggtgaa ggcgctgacg
    15781 cgggagtact tcgcgcacaa caaggtcagg atgatcgacg ggtccgctcc cgttctggac
    15841 tgagccatgg ccaggctgac cgcgaagcag cttcaggacc tcgcctggga gcacgggacc
    15901 ccgctcgtgg tggtgaacca cgacgccatc cgggagaact acgcgacgtt caaacggctc
    15961 ctgccgcggg tgcaggggta ctacgcggtg aaggcgaatc ccgcgcccga gatcgtccgg
    16021 acgctgttca aggccggcgc cagcttcgac gtggcgtccc tgtccgagtt catgctggtg
    16081 tacgacctca tccgccaccg gcccgcgcgg gtgcggcagg acttcgtgtg ggacaagatc
    16141 gtctacgcga acccggtgaa ggccaaggag acgctcgagt ccctcgacaa gtacaagccg
    16201 ctcgtgacct tcgacaacgg gaccgaagtc gtcaagatcc gcgagttcgc gccccgcgcc
    16261 gggctgctgc tgcggatccg ggtgccgaac accgggtcga tggtggagct ctcctcgaag
    16321 ttcgggtgcg accccggcga ggcggtgtcg ctgatggaag aggcccgcgc cgccgggctg
    16381 gaggtggagg ggctgagctt ccacgtcggc agccagtgca cgaacttcga gaactacgtc
    16441 caggcgctga acatgtcggc ggcggtcatg cgggaggccg cgtcgcgggg cttcgacctc
    16501 agggtcctgg acatcggcgg gggcttcccg gtgcgctaca accggcacgt gccgcccttc
    16561 agcctgctgg cccggaggat caacgcggag atcgaccggc tgtttcccgg cgacgtcgag
    16621 atcatcgccg agccgggccg gttcttcgtg gccaccgcca tcacgtccat cgccaccgtc
    16681 atcggcaagg cggtgcgcga cgggaagacc tgctactaca tcgacgacag cgtctaccac
    16741 acgtactcgg gcatcatctt cgaccactgc ccgtaccgca tccacgcctt caagaagggg
    16801 ccggccgagg tgagcgcggt gttcggccgg acctgcgacg ggctggacac gatctcgcag
    16861 gcggagatgc tgccggacct gcagctggac gatctggtgt actcgctgga catcggggcc
    16921 tacagcaacg cgtcggcgac gtggttcaac gggttcccgc cggcgaaggt cgtgcacgtg
    16981 aacgagtgac acgggcccgg ccgctccgcg gccggcccgt cttcagggcg ccgaggcggc
    17041 cccggccgcc ggcgcgtcga aaggacatcc acatgaccgc actcagtctc gctctggccg
    17101 cggcgctcgc ggcgcacgcc ccggcccagg cgccgaaggc ggacgactac gtcgggacgt
    17161 ggaacgtcag gatcaccgat gccgccgaca cgttcgtcag cggcggcttc caggtggaca
    17221 aggcgggaga cggcctgacc ggcggcctgg tgtggcgctc gggcagcttc acgcccgccg
    17281 cgagcgtcga agtcagcgac ggcgccc
//
LOCUS       DAOSIF010000011        17138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_3534_length_17138_cov_4.485453, whole genome shotgun sequence.
ACCESSION   DAOSIF010000011 DAOSIF010000000
VERSION     DAOSIF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17138
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3534_length_17138_cov_4.485453"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1443
                     /locus_tag="PLT35_00945"
     CDS             235..1443
                     /locus_tag="PLT35_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG27819.1"
                     /translation="MLLRALRGSSSYLISVKEHSFAVSVDGAHVVAWDRGARLYSVFR
                     DGVTWRRGLGGTLVEKRRKGSARTHAWPAGDEADRVVDAAAAVAREVCDGIDSSAWRW
                     AGPADAAAVEETRALFESAARFDAASARADARRCELALGRLGILPPDQYLALAVQATE
                     GCSFSTCTFCELYRGRFRIRAPGEFREHVGGVLACLGESARLRSRGVFLGAANALALP
                     MAHLLPIFRVLLEEAAAARRGICAFVDAFTGARKSADDYRVLGRLGLRRVYIGLESGH
                     DPLLSFVRKPGTAAGAVETVRAVKAAGIRAGVIVMTGLGGARFAEGHERDTSAALNAM
                     GLGSGDVLYFSELVEAPSASYPLMAAEAGIRPLTAAEQQAQRSRIRSALRFPSGPPQI
                     AVYDIREFTY"
     gene            1644..2129
                     /locus_tag="PLT35_00950"
     CDS             1644..2129
                     /locus_tag="PLT35_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27820.1"
                     /translation="MAKRPASASKRKAGKAKPLSLRTRIQHDVDRALAAAGIPAAIDA
                     DGWRWMQNPSGNGLIGVVAVLGERGDLTLRAVAPIMPLPKAPGALLRLLKKIAALNYD
                     VVGHGRLAIDSRTVWSVAAQHVSDIGPDDVAACIFDCLWLAQATSPKGSGRITSAASK
                     R"
     gene            complement(2206..2955)
                     /locus_tag="PLT35_00955"
     CDS             complement(2206..2955)
                     /locus_tag="PLT35_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOG27821.1"
                     /translation="MKIATWNVNGVRAREAQLLGWIAREQPDVLCLQELKATREQVPA
                     SLAGLDTYWSYWHGERAYSGVGLLVRKACCSAEPEFAHPGFDHETRIVSAEIGGVNVS
                     SIYVPNGGKDFDAKIRFLEAMIDHAARLTAAGAPVVFCGDLNVAHREIDVHPKERKAV
                     IGQLPEERALFDRLLGAGLVDVGRAQDPANEELFTWWPPWRNMRQRNIGWRLDYVLAS
                     AHLARRAVSCAAYREVGTSDHGPVVATFSPG"
     gene            complement(3118..3876)
                     /locus_tag="PLT35_00960"
     CDS             complement(3118..3876)
                     /locus_tag="PLT35_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27822.1"
                     /translation="MSGCTRLLTLALVALIAAACGGTQQPPAEQAKPAEPAAPAADVA
                     AAMHENLARVTVMQEAVIRGDIEAAAEPAKWIAENQPTAGLPAGQETVLADMKKQAAV
                     VAGATDFKNAATATAMVVSYCGACHKAAQYTPSIPEPVKPAAEPGIRTYMVEHQWAAD
                     LLYQGLVIPSEERWQQGLAAMAVAPLAEKDRPKDAALTKEILEMGKKVQELAGKAKAA
                     PDIGMKVAYYGEYLAGCASCHGLHGKVWGPGIPK"
     gene            4031..5263
                     /locus_tag="PLT35_00965"
     CDS             4031..5263
                     /locus_tag="PLT35_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27823.1"
                     /translation="MLALNVHAGYACRRSGACCTAGWPIPVEADVAARLHADGALPRA
                     PLDARPFLDMRPPLDTRGPAPAGAAGVIAPGPDGACPFFEREPDRLCAIQRRLGHDSL
                     PSACRHFPRIALCEADATRVTLSHFCPTAASMLFRDDIGPIEIVAGASGIAGRDDLEG
                     FDARATIPPLLRPGVAMDAASCRLWERLLLDACNAEHHAAEDALARIARGADGIRTWD
                     ARASALAEHSARIAAGEPGMETGDALASRCSRRTPGRRQAPRWVMPPAQQARLFQAAV
                     ASVPAGLPRPALPLDLDGASRRWVQPAWPDVRRPVNRFLAAHAFGAWSAYLGEGLRTQ
                     AAALAVALAVLRVEAARCAGAAGRALDAALLHAAIRQSDLLLRHLSSADTLVASLDAV
                     ERAAPGDLAGAMGLEMAA"
     gene            5260..6231
                     /locus_tag="PLT35_00970"
     CDS             5260..6231
                     /locus_tag="PLT35_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOG27824.1"
                     /translation="MTPNLHTPGPVRPGARIAFVVNRAARQGRDGRVLADAARLLGER
                     YEVETVTPAGPEGVEAAARAAAAAHDAIVVAGGDGTINRAVNGLAGSALPAGIVPLGT
                     GNDFARAMGIPASPAAAVRVILAGRTRAVDLVRVNGRLFCTVGIIGVPADAALTVANL
                     TGPRSRARCAARRLGDLAYSAAGLWHVLRPGSFVEPVAVAAGGGGAAPSADRLPPGQV
                     PVHAAFVTNTAMLGGGMTVPVGADPADGMIEFAIVPRMPRVRLLAAFRRFTGNRPLPP
                     GILRFERAARATITCSRAVRFSADGDLICEGDRFDVEVLPGALTLIG"
     gene            complement(6420..8204)
                     /locus_tag="PLT35_00975"
     CDS             complement(6420..8204)
                     /locus_tag="PLT35_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27825.1"
                     /translation="MERPAARLLWGCGASLALAAAFGWAACAPGAGTQGGHPGGAPAA
                     PLRIEVTVPESAGEGPFDGRVLVMLSRDPSAEPRFQISDSLNTQQVFGVDVDGLQPGR
                     PAVVDASAPGYPVASLAQVPAGKYTVQALLHKYETFRRSDGHTVKLPMDRGEGQQWSR
                     APGNLYSAPREIDVDPARPGPIRIALDRVIPPIPPPPDTKYVRHVRIQSELLTRFWGR
                     PMHLGANVLLPEGWDAHPDARYPLVISHGHFPYDFGGFRETPPDPDLKPDFSERFRLA
                     GYNRIQQEHAYGFFREWTGPGFPRFIIIEIQHANPYYDDSYAVNSANLGPYGDAIVKE
                     LIPHVEKRFRGIGQGWARFMYGGSTGGWEAMGAQIFYPDEFNGAFIACPDPIDFRAYT
                     VVNLYEDANAYDREGPWKTVARPGHRNWLGHVDTTIRDMNMLELALGANGRSGGQWDI
                     WQAVFSPVGPDGYPKPIWDKRTGVIDREVAAYWREHYDLSHILRRDWAVLGPKLRGKL
                     HIYVGDMDNYYLNNAVYLVEEFLKTADPPYEGEVDYGDRAEHCWNGDHTRPNALSRLR
                     YHQMYVPKILERIRRTAPAGADTKSWRY"
     gene            8633..9586
                     /gene="nadA"
                     /locus_tag="PLT35_00980"
     CDS             8633..9586
                     /gene="nadA"
                     /locus_tag="PLT35_00980"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOG27826.1"
                     /translation="MTMAPSSPPTPDLVQEIARLKRGLNAVILAHYYQEPDIQDIADV
                     IGDSLALAQAAARTEADAIVFCGVHFMAETAAILNPGRLVVVPDLAAGCSLADGCPAE
                     EFERFIRARPGHAVVSYINCSAGVKALSDVICTSSNAVKIVRSIPEGQPIIFAPDQHL
                     GRYVMKQTGREMVLWPGFCSVHVLFSARQIVRLKAEHPGALVLAHPECEEPVLQLADH
                     VASTSGLLAFTKASPARAFIVATEPGIIHQMKKASPDKMFIPAPPDHGCACNECPHMR
                     LNTLEKVRDCLRDRSPAVSVPEGLRARARAPLDRMLELSRA"
     gene            9609..10526
                     /gene="nadC"
                     /locus_tag="PLT35_00985"
     CDS             9609..10526
                     /gene="nadC"
                     /locus_tag="PLT35_00985"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOG27827.1"
                     /translation="MATDPFRQSSPAALDEAFVEREVRRFLAEDAGPGDVTSERVVPK
                     AARARASMVARQPCVVAGLPIARAAFRAADPAVAVSLCASDGDAVAAGAVLAAVEGPA
                     AGILGAERVALNLVQRLSGIATLTRRYVEAVAGTGASVSDTRKTTPGLRAFEKYAVRV
                     GGGRNHRRGLFDAILIKDNHRAIAGGAEAAIAAARRGRRRMPIQIEVDSLSDLRRALD
                     LGVDAVLLDNMTPHDAAEAVALARAHPHGRACWIEASGGITLATVRAYAEAGVDTVSL
                     GAITHSAPAVDIALDFEAALSRESDHDEP"
     gene            10513..>11440
                     /locus_tag="PLT35_00990"
     CDS             10513..>11440
                     /locus_tag="PLT35_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG27828.1"
                     /translation="MTSRDTDVLVIGSGLAGCAAALAAARWGCRVLVLTKGARPEESN
                     TLYAQGGIVYRGAADSPEALAADIMAAGGGLCHPPAVELLAREGPRLVDAVLIDGAGV
                     AFDRAPDGSGGLDLTAEAAHSTARIVHHADTTGRAIEEAMVAALAADPRIEVRTGQTA
                     IDLLTLSHHSERRTDVYAPPTCVGAYVFDQASRRVDTVLARETILATGGLGRIFLHTT
                     NPAGACGDGVAMAYRAGARCINMHFVQFHPTTLFAGEERFLLSESMRGEGAKLVDQGG
                     REFMRDYHPDGSLAPRDVVARGIHQMMHETGSP"
     assembly_gap    11441..11450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11451..12084
                     /locus_tag="PLT35_00995"
     CDS             <11451..12084
                     /locus_tag="PLT35_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG27829.1"
                     /translation="AYLDISHKDPAWVRARFPRIRERCLEEGFDIAAEPIPVVPAAHY
                     SCGGVAVDEWGRSSLHRLRAVGEVACTGLHGANRLASTSLLECLVWGTRAGEAAAAAV
                     TSGAPYYLPRIAAWKQETEPVDPALVAQDWLTIRQTMWNYVGLVRSRRRLDRAHQILR
                     ELHLEIGRFYAKSELSDALIGLRNGIQTALVVLLAAIEAKASAGAHYRVD"
     gene            complement(12214..13494)
                     /locus_tag="PLT35_01000"
     CDS             complement(12214..13494)
                     /locus_tag="PLT35_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOG27830.1"
                     /translation="MRLIDRLPRPLVVFFTVIGPGFITAMVDNDAGGIYTYSQAGAMF
                     GYVLLWTLPPIALLLVVTQEMNARMGAVTGKGLSDLIREEFGLRTTFGLMALLVVANL
                     TNVMANFAGVASALELFRVSRYVSVPLAAAAVWFLVVKGTYDSVEKIFLMACVTYIAY
                     VIAAVLINPDWEASALASVRPVFSWDPAYVMLVVGLVGTSVAPWMQFYLQAAVVEKGI
                     TVKDYAESRIEVIVGCVVMIAVAFFIIVACAAGIWSHGPREITSATDAATALRPFGEY
                     AYLLFSAGLLNASLFAASILPLSTAYSVCEGLGFESGVNKRFREAPVFYWLYTGLIVV
                     GAAVVLVPGFPLVRMILLAQVVNGILLPFVLIFMLRLVNNTGLMHEWTNSRFYNVIAW
                     TAVVVLIGMTVILLGATLREMYFPNARAVIELAP"
     gene            complement(13497..14744)
                     /locus_tag="PLT35_01005"
     CDS             complement(13497..14744)
                     /locus_tag="PLT35_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG27831.1"
                     /translation="MASNLLFLTELMGMRVCDVKGRRLGRIRDACLAPRVDKVRIDQF
                     LVGGEWNWWIARHDQISSISLDGIRLRDEQLVPYHDDVWTLRMARDLLDKQIIDAKGR
                     KVVRVNDITFELNRAGDHDELWVLEVDVGMRSVVRRLCQGILPPRLVRRLMVRIAPNS
                     IPWEFCNIIEPDPQRRLRLNIDLKGLEDIHPADLADIVEELSHEGREAVFETIDSEVA
                     AETLSEVEDTKTQAAILESLDADRAADIVGEMSPDEAADVLAEMEESSSEAILEEMEA
                     EEKTDVEELLGFDEKSAGGLMNSEFIAAPEGGTVADALRIVRENEEHVDTLTNVFLVD
                     GDGRLTGSLPVGRLLLVEPTALLKPLAGADDVVSVPVAARHTRVAELVDKYNLMALPV
                     VDEAGALVGVVTADDVISMLRQD"
     gene            15071..15511
                     /locus_tag="PLT35_01010"
     CDS             15071..15511
                     /locus_tag="PLT35_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27832.1"
                     /translation="MSDRKYRQRGYMDDDRDRAPRPPARERERAPGPRPRGDRPEGPR
                     TPNMPGFRSVIRCHRCGALVTAAIAVGTACARCGAALHCCAQCESFDPGARYECLQPL
                     TARVAPKDAFTTCSLFEPRSTVERETGSARQSTARTAFDDLFKI"
     gene            15712..16674
                     /locus_tag="PLT35_01015"
     CDS             15712..16674
                     /locus_tag="PLT35_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOG27833.1"
                     /translation="MITEITAAELDPERFVAEQAARIRAAVGGGTAINALSGGVDSSV
                     VTMIGHRALGPRLRTVFVDNGLMREGEPEQVVGMFRRLGVEIEVVDARAEFLAALAGL
                     TDPEQKREAVTQTFYTRVFGRLVRESGARFLLQGTILTDVDETVAGIKRQHNVFEQLG
                     IDPQAAFGYRILEPLVQLRKDAVRTVGRSLGLPESMFARIPFPGPALAARIIGEVTAE
                     RLAAVRRATSIVERALGGLGAFQYLAVLHEDRVTGMRDGRRDFGQQIEVRCWDSVDGR
                     RATPTRVPFETLESLAAEILRDVPGVVSVTYNIAAKPPSTIEAV"
     gene            complement(16687..>17138)
                     /locus_tag="PLT35_01020"
     CDS             complement(16687..>17138)
                     /locus_tag="PLT35_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOG27834.1"
                     /translation="RAMLDPVTEIARYGAPKGITVSLETGQETGAQLAAFLDRITVAR
                     VGVNFDPANLIIYGVDAPSAALRHVVSRVTSVHVKDALLPASPGAMGQEVRPGEGRAE
                     LAACFEILRRAGFTGALIVENYVARGAGTDPMDELRRAVAFIRAAGW"
BASE COUNT         2336 a         6230 c         6111 g         2451 t
ORIGIN      
        1 gagcacgccg ccggcatcca ggaagaccgt gcggtagcgc gtggacagag tgcggacgga
       61 ggtcatcacc agtccttcag tgtctcatcg cgcctccgcc ctgtcgagcg gcgccgtcgc
      121 ccgcgggctg aagcgtatac taggcgcgcc ggtgaaccgg gttccggacc ccgcgtccag
      181 gcctttggag cccgagcccc gagtcccgag ccccgatccc cgagccccga tcccatgctc
      241 ctccgcgcgc tccgcggctc gtcgtcctac ctcatcagcg tcaaggagca ttcgttcgcc
      301 gtgagcgtgg acggcgcgca cgtggtggcg tgggaccgcg gcgcgcgcct ctacagcgtg
      361 ttccgggacg gcgtgacgtg gaggcgcggg ctcggcggga cgctggtcga gaagcgccgg
      421 aagggaagcg cgcgcacgca cgcctggccg gccggcgacg aggcggaccg cgtggtcgac
      481 gccgccgccg ccgtcgcgcg ggaggtgtgc gacggaatag attcctcggc ctggaggtgg
      541 gccgggccgg cggacgcggc cgccgtcgaa gaaacgcgcg ccctcttcga atcggccgcg
      601 cgtttcgacg cggcctcggc gcgcgccgac gcgcgccggt gcgagctggc gctcggccgc
      661 ctcggcatcc tgccgcccga tcagtatctg gcgctggcgg tgcaggcgac cgaaggctgc
      721 tcgttcagca cctgcacgtt ctgcgagctg taccggggcc gcttccggat ccgggcgccc
      781 ggcgagttcc gcgagcacgt cggcggcgtg ctggcctgcc tgggcgagtc ggcccggttg
      841 cggtcgcgcg gcgtcttcct cggcgcggcc aacgcgctcg ccctgccgat ggcgcacctg
      901 ctgccgatct tccgcgtcct gctggaggaa gcggcggccg cccgccgcgg catctgcgcc
      961 ttcgtcgacg cgttcaccgg cgcccggaag agcgccgacg actaccgcgt gctcggccgg
     1021 ctcggcctca ggcgcgtcta catcggcctc gagtccggcc acgatccgct gctgtcgttc
     1081 gtgcgcaagc ccgggacggc cgccggcgcc gtcgagacgg tccgcgccgt caaggccgca
     1141 ggcattcgcg ccggggtgat cgtcatgacc ggcctcggcg gcgcgcggtt cgccgagggc
     1201 cacgagcgcg atacgagcgc ggcgctgaac gcgatggggc tcggaagcgg ggacgtgctc
     1261 tacttcagcg agctggtcga ggcgccgtcg gcgtcgtacc cgctgatggc cgccgaagcg
     1321 gggatcaggc cgctgacggc ggcggagcag caggcccagc ggtcgcgcat ccgatccgcg
     1381 ctccgctttc cctccggccc gcctcagatc gccgtctacg acatccgcga gttcacctac
     1441 tgagaacgga gcccggtcgt cgtctttggg gagttgctgg ccgccgcggt cggagccatg
     1501 atcgggatga cgaaggcgct cgccgaggca gggcgaagga gcggcgcgcg ccgggaaggg
     1561 gcggcaggcc gcttggcggg cgccgcgccc ggatgtgagc gtcacggcgg gcggccgttt
     1621 gcccgcgcca caaggagcct gtcatggcga aacgaccagc gtccgcgtcg aagagaaagg
     1681 ccggcaaggc gaagccgctc tcgctgcgca cccggatcca gcacgatgtc gatcgcgcgc
     1741 tggccgcggc gggcatcccg gcggccatcg acgccgacgg ctggcgctgg atgcagaacc
     1801 cgtccgggaa cggcctcatc ggcgtcgtgg cggtgctggg cgagcgcggc gatctcacgc
     1861 tgcgcgccgt ggcgcccatc atgccgctgc cgaaagctcc gggcgcgctg ctgaggctgc
     1921 tgaagaagat cgccgcgctg aactacgacg tggtcgggca cggccgcctc gccatcgaca
     1981 gccgcactgt ctggtccgtc gccgcccagc acgtgtctga catcggcccc gatgacgtgg
     2041 cggcgtgcat cttcgattgc ctgtggctgg cccaggccac ctcaccgaag gggtcgggtc
     2101 gaattacgtc cgcggcttcg aaacgatgac aaatctgtcg gctgcggacg ctgcgcacga
     2161 atttgtaatc tggcgaggtt ccactacaaa aagcgtcccg accccttagc ctggggagaa
     2221 ggttgcgacg acgggcccgt gatcgctcgt gccgacttcg cggtacgccg cgcacgacac
     2281 cgcccgccgc gccaggtgcg cgctcgcgag cacgtagtcg agccgccacc cgatgttgcg
     2341 ctggcgcatg ttgcgccacg gcggccacca cgtgaacaac tcctcgtttg ccggatcctg
     2401 cgcgcgtccc acgtccacca ggccggcgcc gagcagccga tcgaagagcg cccgctcctc
     2461 cggcaactgg ccgatgaccg ccttgcgctc cttcggatgc acgtcgatct cgcggtgcgc
     2521 gacgttcaga tcgccgcaga agactacggg ggcgcccgcg gcggtcagcc gggctgcgtg
     2581 atcgatcatg gcctcgagga agcggatctt cgcgtcgaag tccttgccgc cgttcggcac
     2641 gtagatcgac gacacgttca cgccgccaat ctcggccgag acgatgcgcg tctcgtggtc
     2701 gaacccgggg tgcgcgaact ccggctcagc cgagcagcac gccttgcgga cgagcagccc
     2761 gacgccggaa tacgcgcgct cgccgtgcca gtacgaccag taggtgtcga ggccggcgag
     2821 cgacgccggg acctgttcgc gggtggcctt gagttcctgc aggcacagga cgtcgggctg
     2881 ctcgcgcgcg atccagccga ggagctgcgc ttcgcgggcg cgcacgccgt tcacgttcca
     2941 ggtggcgatc ttcatggctc ttgagccgat gatagcaccc ggattcgcgc gcagggcgcg
     3001 gccagccgcc ccggcctcgg tgggaacccc tgcttcgagc caggcctggc acgccatccg
     3061 cgcgggggta cccggcgggc gccgccgccc ggcccgtgca actcgccccg cagaacccta
     3121 cttcggaatg cccggccccc agaccttgcc gtgcaggccg tggcagctcg cgcacccggc
     3181 gaggtactcg ccgtagtagg cgaccttcat gccgatatcg ggcgccgcct tggccttgcc
     3241 cgcgagttcc tgaaccttct tccccatctc caggatctct ttcgtcagcg cggcgtcctt
     3301 cggccggtcc ttctcggcga ggggagcgac ggccatggcc gccagcccct gctgccaccg
     3361 ctcctcggat gggatcacga ggccctggta gaggaggtcc gccgcccact ggtgctcgac
     3421 catgtaggtc ctgatgcctg gctccgcggc cggcttcacc ggctccggga tgctcggggt
     3481 gtactgcgcg gccttgtggc aggcgccgca gtaggagacg accatggccg tggcggttgc
     3541 ggcgttcttg aagtcggttg cgccggcgac gaccgcggcc tgcttcttca tgtcggcgag
     3601 gaccgtctcc tggccggccg gaagcccggc cgtcggctgg ttctcggcga tccacttcgc
     3661 cggctcggcg gcggcctcga tgtcgccgcg gatcacggct tcctgcatga cggtgacgcg
     3721 cgcgaggttc tcgtgcatgg cggcggccac gtcggccgcg ggagctgccg gttcggccgg
     3781 cttggcctgc tcggcaggcg gctgctgggt cccgccgcac gcggcggcga tgagggcgac
     3841 gagcgcgaga gtcagaagac gggtgcatcc gctcatgaag ggcctccgca gggacgccga
     3901 cattctttac ccgcgcgctt ccgccgtcaa tgggagcgtg gggtcgggtc tcgaaccact
     3961 gatgtcggcc tgcggattcg agagccgacc tcagtaattc gagacctgac cccgctatga
     4021 tgaggcggtg gtgctcgcgc tcaatgtcca cgccggctac gcgtgccgtc gctccggcgc
     4081 atgctgcacc gccgggtggc cgatcccggt cgaggccgat gtcgcggccc ggctgcacgc
     4141 ggacggcgcg ctgccgcgcg cgcccctgga cgcgcgcccg ttcctggaca tgcgcccgcc
     4201 cctggacacg cgcggtcccg cgccggccgg cgccgccggc gtcatcgcgc caggcccgga
     4261 cggcgcctgc cccttcttcg agcgcgagcc ggatcgcctc tgcgcgatcc agcgccgcct
     4321 gggccacgac agcctcccgt cggcctgccg ccatttcccg cgcatcgcgc tctgcgaggc
     4381 cgacgccacg agagtcacgc tgtcgcactt ctgtccgacg gccgcatcga tgctgttccg
     4441 cgacgacatc gggccgatcg agatcgtggc cggcgcctct ggcatcgccg gccgcgacga
     4501 tctcgagggc ttcgacgcgc gcgccacaat cccgcccctg ctccggccgg gcgtcgcgat
     4561 ggatgcggca agctgccggc tctgggaacg gctccttctc gacgcctgca acgcggagca
     4621 ccacgccgcc gaagatgcgc tcgcccgcat cgcgcgcggg gcggacggca tcaggacctg
     4681 ggacgcgcgg gcctcggccc ttgccgagca ctcggcccgg atcgccgcag gagagccagg
     4741 gatggagacc ggggacgccc tggcgtcgcg atgttcgcgg cgcacgccgg gaaggcgaca
     4801 ggcgccgcgc tgggtgatgc cgcccgcgca gcaggcccgc ctgttccagg ccgctgtcgc
     4861 gtcggtcccg gcagggctcc cgcggccggc tcttccattg gatctggacg gcgccagccg
     4921 ccggtgggtg cagccggcat ggccggacgt gcggcgtccc gtcaaccgct tcctggccgc
     4981 gcacgccttc ggggcgtgga gcgcctacct cggcgagggg cttcgcacgc aggccgccgc
     5041 gctggcggtt gcgctcgcgg tgcttcgcgt cgaagcggcc cgctgcgcgg gcgccgccgg
     5101 gcgcgcgctc gatgcggcgc tgctccatgc ggccatccgg cagtccgacc tgctgctgag
     5161 gcacttgtcg agcgcggaca cgctcgtggc gagcctggat gcggtcgagc gcgcggcgcc
     5221 cggcgatctc gccggcgcga tgggcctgga gatggccgca tgacaccgaa cctgcacacg
     5281 ccgggcccgg tgcgccccgg cgcccgcatc gcgttcgtcg tgaacagggc cgcccgccaa
     5341 ggccgcgacg gccgcgtcct cgccgacgcg gcccggctgc tcggggagcg ctacgaggtc
     5401 gagactgtca cgccggccgg gccggagggc gtcgaagcgg ccgcgcgcgc ggcggcggcc
     5461 gcgcacgatg cgattgtcgt ggcaggcggc gacggcacga tcaaccgcgc cgtcaacggg
     5521 ctcgccgggt cggcgctgcc cgccggcatc gtgcccctgg gcaccggcaa cgacttcgcc
     5581 cgagccatgg gcattcccgc gtcgcccgcg gccgcggtcc gcgtcatcct cgccggccgc
     5641 acgcgggccg tcgatctcgt ccgcgtcaac ggccgtctgt tctgcaccgt cggcatcatc
     5701 ggcgtcccgg ccgacgccgc gctgaccgtg gccaatctca ccgggccgcg ttcgcgcgcg
     5761 cggtgtgcgg cgcgccggct cggggacttg gcctacagcg ccgcggggct ctggcacgtg
     5821 ctgaggccag gcagcttcgt ggagcctgtc gcggtggccg ctggcggcgg cggcgcggcg
     5881 ccgtcagccg accgcttgcc gcccggtcag gttccggtgc acgccgcgtt cgtgacgaac
     5941 accgccatgc tgggcggcgg catgaccgtg cccgtcggcg cagatcccgc cgacggcatg
     6001 atcgaattcg cgatcgtccc gcgcatgccg cgcgtgcgcc tgctggcggc gttcaggcgg
     6061 ttcaccggca accggcccct gccgccgggc atcctgcggt tcgagcgcgc ggcgcgggcc
     6121 accatcacct gctcgcgcgc cgttcgcttc tcggccgacg gcgatcttat ctgcgaaggg
     6181 gatcgcttcg acgtcgaggt cctgcccggg gccctcacgc tcatcggata gctcggggct
     6241 cggggctcga gactcggggc tcgggattcg ggaactgcag cgcgacagat tctgaattcc
     6301 cgccttcgct agacggcctg ccggccggct ggggcgggca agctggattc tgactcctgt
     6361 acctcctcta cctcctgcct cggcttgccc ctctctgacg agtccccagt gcctggcccc
     6421 tagtacctcc agctcttcgt gtccgcgccg gccggggccg tcctgcggat ccgctcgagg
     6481 atcttcggca cgtacatctg gtggtaccgc agccgcgaca gcgcgttcgg ccgcgtgtga
     6541 tcgccgttcc agcagtgctc ggcgcgatcg ccgtagtcca cttcgccttc gtacggcgga
     6601 tccgccgtct tcaggaactc ctcgacgagg tagaccgcgt tgttgaggta gtagttgtcc
     6661 atgtcgccga cgtagatgtg cagcttgccg cggagcttcg gcccgagcac ggcccagtcg
     6721 cggcgcagga tgtgcgacaa gtcgtagtgc tcgcgccagt acgccgccac ctcgcggtcg
     6781 atgacgcccg tccgcttgtc ccagatcggc ttcgggtacc cgtcgggccc gaccggcgag
     6841 aagaccgcct gccagatgtc ccattggccg cccgaccggc cgttggcgcc gagcgccagc
     6901 tcgagcatgt tcatgtcgcg gatcgtcgtg tcgacgtggc cgagccagtt gcggtggccg
     6961 ggccgcgcca ccgtcttcca cgggccctcg cggtcgtacg cgttcgcgtc ctcgtagagg
     7021 ttcacgaccg tgtacgcgcg gaagtcgatc gggtccgggc aggcgatgaa ggcgccgttg
     7081 aactcgtcgg ggtagaagat ctgcgcgccc atcgcctccc agccgccggt ggacccgccg
     7141 tacatgaacc gcgcccagcc ctggccgatg ccccggaacc gcttctcgac gtgcgggatg
     7201 agctccttca cgatggcgtc gccgtacggc cccaggttcg cggagttcac cgcgtacgag
     7261 tcgtcgtagt acgggttggc gtgctggatc tcgatgatga tgaaccgggg gaagcccggg
     7321 ccggtccatt ccctgaagaa cccgtacgcg tgctcctgct ggatccggtt gtagcccgcc
     7381 agccggaacc gctcgctgaa gtccggcttc aggtccggat cgggcggcgt ctcgcggaag
     7441 ccgccgaagt cgtacggaaa gtgcccgtgg ctgatcacga gggggtagcg cgcgtccggg
     7501 tgcgcgtccc agccctcggg cagcagcacg ttcgcgccga ggtgcatcgg ccgcccccag
     7561 aacctggtca gcagctcgct ctggatgcgc acgtgcctga cgtacttcgt gtccggcggc
     7621 ggcgggatcg gggggatgac ccggtcgagc gcgatccgga tcggccccgg cctggccgga
     7681 tcgacgtcga tctcgcgcgg ggcgctgtag aggttgccgg gcgcgcggct ccactgctgg
     7741 ccctcgcccc ggtccatcgg cagcttcacc gtgtggccgt cggaccgccg gaacgtctcg
     7801 tacttgtgca gcagcgcctg gaccgtgtac ttccccgcgg gcacctgcgc caggctggcg
     7861 acggggtagc ccggcgccga ggcgtcgacc accgccggcc ggcccggttg caggccgtcc
     7921 acgtccacgc cgaacacctg ctgcgtgttc aggctgtcgc tgatctggaa cctcggctcc
     7981 gcggacgggt ccctggacag catgaccagc acgcggccgt cgaacgggcc ctcgcccgcc
     8041 gactccggca ccgtcacctc gatgcgcagc ggggccgccg gcgcgccgcc cgggtggccg
     8101 ccctgcgtgc cggctcccgg cgcgcacgcg gcccagccga acgccgccgc cagcgcgagc
     8161 gatgcgccgc aaccccagag aagccgcgcc gccggtcgtt ccatgtccat cctccaatcg
     8221 tccgttcgcc gccgccgtac ggaagccccc gagcgcggcg gcaggtcgtg tggcgaacca
     8281 tgcgagcacg ccgggacggc cccgcgacga acgcaccgga ggcgccgaag tcccggatgg
     8341 ggtctcggag gccccggcct tccgccgaca gcaccctgaa ggtgcgttcg tccggggcgt
     8401 cgagcggttc gcgacacggc atgccgccgt gctccgcgat cccgccgtgc tccgcgatcc
     8461 cgccgtcgcc ggcaggaccc gctgccgggc gggtggcccg cacctgcccg gcacggtcac
     8521 cgcgcccgcg cgggtgacgg ctccggcgct cccgcccgac gcgcagtgta gcaccggccg
     8581 ccgccgcccg cgccgctggc ccgcgccctg gatctgtcgt accatcgtca tcatgacgat
     8641 ggcgccatcg tcgcctccga cgccggatct cgtccaggag atcgcgcgcc tgaagcgcgg
     8701 gctgaacgcc gtcatcctcg cgcactacta ccaggagccc gacatccagg acatcgccga
     8761 cgtcatcggc gacagcctcg cgctggccca ggccgccgcg cgcaccgagg ccgacgccat
     8821 cgtcttctgc ggcgtgcact tcatggccga gaccgcggcc atcctgaatc ccggccggct
     8881 cgtcgtcgtg cccgatctcg ccgccgggtg ctcgctcgcc gacggctgcc cggcggagga
     8941 gttcgagcgg ttcatccgcg cgcgcccggg ccacgccgtc gtcagctaca tcaactgcag
     9001 cgccggcgtg aaggcgctga gcgacgtcat ctgcacgtcg agcaacgccg tgaagatcgt
     9061 ccgcagcatc ccggagggcc agcccatcat cttcgcgccc gaccagcacc tgggccggta
     9121 cgtgatgaag cagaccggcc gcgagatggt gctctggccc ggcttctgct cggtgcacgt
     9181 gctcttttcc gcccgccaga tcgtccgcct gaaggcggag caccccggcg ccctcgtgct
     9241 cgcgcacccg gagtgcgagg agccggtgct gcagctggcg gatcacgttg cgtccacgtc
     9301 ggggctgctg gcgttcacga aggcgagccc cgcgcgcgcg ttcatcgtgg ccaccgagcc
     9361 cgggatcatc caccagatga agaaggccag cccggacaag atgttcatcc ccgcgccccc
     9421 cgaccacggg tgcgcgtgca acgagtgccc ccacatgcgc ctgaacacgc tcgagaaggt
     9481 gcgcgactgc ctgcgcgacc ggtcgccggc cgtgtcggtg cccgagggcc ttcgcgcgcg
     9541 cgcgcgcgcg ccgctcgatc gcatgctgga gttgagccgg gcctgacgcc gccgggccga
     9601 ggcgagccat ggcgaccgat ccgttccgcc agtcctctcc tgccgcgctg gacgaggcgt
     9661 ttgtcgagcg cgaagtgcgc cgcttcctgg ccgaggacgc cggcccgggc gacgtgacga
     9721 gcgagcgcgt cgtgccgaag gcggcccggg cgcgggcgtc gatggtggcg cggcagccgt
     9781 gcgtcgtcgc gggccttccg atcgcgcgcg cggcgttccg cgcggccgat ccggccgtcg
     9841 ccgtgtcgct gtgcgcgtcg gacggcgatg ccgtggccgc cggcgccgtt ctcgcggccg
     9901 tcgagggccc ggctgcgggc attctgggcg ccgagcgcgt ggcgctcaac ctcgtccagc
     9961 gcctctcggg catcgccacc ctcacgcggc gctacgtcga ggccgtcgcg ggcacgggcg
    10021 cgtccgtgtc cgacacgcgc aagaccacgc cggggctgcg ggcgttcgag aagtacgcgg
    10081 tgcgcgtggg cggcgggcgc aatcaccgcc gcggcctctt cgacgcgatc ctcatcaagg
    10141 acaaccaccg cgccattgcc ggcggggcgg aggccgcgat tgcggcggcc cggcgcgggc
    10201 ggcggcggat gcccatccag atcgaggtgg actcgctctc cgatctgcgg cgcgcgctcg
    10261 atctcggcgt ggacgccgtc ctgctcgaca acatgacgcc ccacgacgcg gccgaggccg
    10321 tcgcgctggc gcgcgcgcac ccgcacgggc gcgcctgctg gatcgaggcg tcgggcggca
    10381 tcaccctcgc caccgtccgg gcgtacgccg aagcgggcgt ggacacggtg tcgctgggcg
    10441 cgatcacgca ctcggcgccc gccgtcgaca tcgccctcga cttcgaggcc gccctctccc
    10501 gggagtccga ccatgacgag ccgtgacacc gacgtcctcg tcatcggcag cggcctcgcc
    10561 ggctgcgccg ccgcgctggc ggccgcgcgg tggggctgcc gcgtgctcgt cctcacgaag
    10621 ggcgcgaggc cggaggagag caacaccctg tacgcccagg gcggcatcgt ctaccgcggc
    10681 gcggcggact cgccggaggc gctggcggcc gacatcatgg ccgcgggcgg cgggctctgc
    10741 cacccgccgg ccgtcgagct gctcgcgcgc gaggggccgc ggctcgtcga cgcggtgctc
    10801 atcgatggcg cgggcgtggc cttcgaccgg gcgcccgacg ggtcgggcgg cctggacctc
    10861 acggcggagg ccgcccactc gacggcgcgg atcgtccacc atgccgacac gaccgggcgc
    10921 gcgatcgagg aggcgatggt ggcggcgctc gccgccgatc cgcgcatcga ggtgcggacc
    10981 ggccagacgg ccatcgacct cctcacgctc tcgcaccact ccgagcgccg gaccgacgtc
    11041 tacgcgccgc ccacctgcgt cggcgcctac gtgttcgacc aggcgtcgcg tcgggtggac
    11101 accgtgctgg cgcgcgagac aatcctggcc acggggggcc tggggcgcat cttcctgcac
    11161 accaccaacc ccgccggcgc gtgcggcgac ggcgtggcca tggcgtaccg cgccggggcg
    11221 cgctgcatca acatgcactt cgtgcagttc cacccgacga cgctcttcgc gggggaggag
    11281 cggttcctgc tctcggagtc gatgcggggc gagggcgcga agctggtgga ccagggcggg
    11341 cgcgagttca tgcgggacta ccaccccgac gggtcgctcg cgccgcgcga cgtcgtggcg
    11401 cggggcatcc accagatgat gcacgagacc ggctcgccgt nnnnnnnnnn cgcgtacctc
    11461 gacatcagcc acaaggaccc cgcctgggtc cgcgcgcgct tcccgcgcat ccgcgagagg
    11521 tgcctcgagg agggcttcga catcgccgcc gagcccattc ccgtcgtgcc ggcggcgcac
    11581 tacagctgcg gcggcgtggc ggtggacgag tggggccgct ccagcctgca caggctgcgc
    11641 gcggtggggg aggtcgcctg caccggcctg cacggcgcga accgcctcgc cagcacgtcg
    11701 ctcctcgagt gcctcgtctg gggaacccgc gcgggcgagg ccgcggcggc ggccgtcacc
    11761 tccggcgcgc cgtactacct gccgcgcatc gccgcgtgga agcaggagac ggagcccgtc
    11821 gatccggcgc tcgtcgccca ggactggctc accatccgcc agaccatgtg gaactacgtc
    11881 ggtcttgtcc gcagccggag gcgcctcgac cgcgcccacc agatcctgcg cgagctccac
    11941 ctcgagatcg gccgcttcta cgcgaagtcc gagctgagcg acgcgctcat cggtttgcgc
    12001 aacggcatcc agacggccct cgtcgttctg ctcgcggcca tcgaggcgaa ggccagcgcc
    12061 ggggcgcatt accgggtcga ctaggggcta ggggctgggc gcctagggct agtcaggctg
    12121 ggtgctcagc gtgacacgcc acccacgcgg cggcagccgc gtcagactag cgcctagcgc
    12181 ccagcccctg gcccctagca cagccccgcc ccctcagggc gccagctcga tcaccgccct
    12241 ggcgttgggg aagtacatct cgcggagggt ggcgccgagg aggatgaccg tcatgccgat
    12301 gaggaccacc accgccgtcc aggcgatgac gttgtagaag cgcgagttcg tccactcgtg
    12361 catcagcccg gtgttgttga cgagccggag catgaagatc agcacgaagg gcagcaggat
    12421 cccgttgacc acctgcgcga gcaggatcat ccgcaccagg gggaagcccg ggaccagcac
    12481 gaccgccgcc ccgacgacga tgaggcccgt gtacagccag tagaacaccg gggcctctcg
    12541 gaaccgcttg ttcacgcccg actcgaaccc caggccctcg cacaccgagt acgccgtcga
    12601 gagcgggagg atgctcgcgg cgaagagcga cgcgttcagc aggccggcgc tgaacagcag
    12661 gtaggcgtac tcgccgaagg ggcgcagcgc cgtcgccgcg tcggtggcgc tggtgatctc
    12721 gcgcgggccg tggctccaga tccccgccgc gcaggcgacg atgatgaaga acgcgacggc
    12781 gatcatgacg acgcagccga cgatgacctc gatgcgcgac tcggcgtagt ccttgacggt
    12841 gatgcccttc tcgacgacgg ccgcctgcag gtagaactgc atccacggcg cgaccgaggt
    12901 gcccaccagt ccgacgacca gcatgacgta ggccgggtcc cagctgaaga ccggccgcac
    12961 gctggccagg gccgacgcct cccaatcggg gttgatgagc acggcggcga tgacgtaggc
    13021 gatgtaggtc acgcaggcca tcaggaagat cttctcgacg ctgtcgtacg tgcccttgac
    13081 caccaggaac cagaccgcgg cggccgccag cggcaccgag acgtaccggc tcacgcggaa
    13141 cagctccagc gcgctggcga cgccggcgaa gttggccatg acgttcgtca ggttcgcgac
    13201 aacgagcagg gccatcagcc cgaaggtcgt gcggaggccg aactcctcgc ggatgaggtc
    13261 ggacaggccc ttgcccgtca ccgcgcccat ccgcgcgttc atctcctgcg tcaccaccag
    13321 cagcagcgcg atgggcggca gcgtccacag gagcacgtag ccgaacatcg cgccggcctg
    13381 cgagtaggtg tagatgccgc ccgcgtcgtt gtccaccatc gcggtgatga agcccggccc
    13441 gatgacggtg aagaagacga cgagcgggcg gggcaggcgg tcgatcaggc gcacggctag
    13501 tcctgtctca gcatcgagat gacgtcgtcg gcggtgacga cgccgacgag ggcgcccgcc
    13561 tcgtcgacga ccggcagggc catgagattg tacttgtcca ccagctccgc gacgcgcgtg
    13621 tgcctggcgg ccaccggcac cgacaccacg tcgtcggcgc ccgcgagggg cttgaggagc
    13681 gccgtcggct cgaccagcag caggcggccc acgggcagcg atccggtgag ccgcccgtcg
    13741 ccgtcgacga ggaacacgtt cgtcagcgtg tccacgtgct cctcgttctc gcggacgatc
    13801 cgcagggcgt cggcgaccgt gccgccctcg ggcgccgcga tgaactcgct gttcatcaaa
    13861 ccgccggcgg atttctcgtc gaacccgagc agctcctcga cgtccgtctt ctcctcggcc
    13921 tccatttcct cgaggatcgc ctcggaggag gactcctcca tctcggcgag gacgtcggcc
    13981 gcctcgtcgg gggacatctc gccgacgatg tcggcggcgc gatcggcgtc gagcgactcg
    14041 aggatggccg cctgggtctt cgtgtcctcg acctccgaga gcgtctcggc cgccacctcg
    14101 ctgtcgatcg tctcgaacac ggcctcgcgc ccctcgtggc tgagctcctc gacgatgtcg
    14161 gccaggtcgg cggggtggat gtcctcgagg cccttcaggt cgatgttgag gcggagccgg
    14221 cgctgcgggt cgggctcgat gatgttgcag aactcccagg ggatcgagtt cggggcaatg
    14281 cgcaccatca ggcggcgcac caggcggggc ggcaggatgc cctggcagag gcggcggacg
    14341 acgctgcgca tgcccacgtc cacctcgagc acccagagct cgtcgtggtc gccggcgcgg
    14401 ttcagctcga acgtgatgtc gttgacccgg acgaccttgc ggcccttcgc gtcgatgatc
    14461 tgcttgtcga gcaggtcccg cgccatccgc agcgtccaca cgtcgtcgtg gtagggcacg
    14521 agctgctcgt cgcggaggcg gatgccgtcg agcgaaatcg acgagatctg gtcgtggcgg
    14581 gcgatccacc agttccattc gccgcccacg aggaactgat cgatgcggac cttgtccacg
    14641 cggggcgcga ggcaggcgtc gcgaatccgg ccgaggcggc ggcccttcac gtcgcagacc
    14701 ctcatgccca tcaattcggt aagaaagagc aggttgctgg ccatggcacc gctcctcctg
    14761 gcattgggcg tgagtggacg gcaggcgccg ggatccttcc cttatagcag aacccgccgg
    14821 gactgccagg gggaaaacag caacctggtg cccgggttcc ggggtcccgg ggtcagatct
    14881 cgaattcctg cgggcaggcc gggagcccgc aggaattcga gatctgaccc cggatgtggg
    14941 ggaggccggg agcccgcagg aattcgagat ctgaccccgg ctgtgcgggc aggtcgggag
    15001 cccgcaggaa ttcgagatct gaccccggat ccccggatgt gcgggcctca tctggggtac
    15061 agtgtgcggc atgagcgaca ggaagtaccg ccagcggggc tacatggacg acgaccgcga
    15121 ccgcgcaccg cggccccccg cccgggagcg cgagcgcgcc ccggggccga ggccgcgcgg
    15181 cgaccggccg gagggcccgc gcacgccgaa catgccgggc ttccggtccg tcattcgctg
    15241 ccaccgctgc ggcgcgctgg tcacggcggc catcgccgtc ggcacggcgt gcgccaggtg
    15301 cggcgccgcg ctccactgct gcgcccagtg cgagtcgttc gacccgggcg cgcgctacga
    15361 gtgcctgcag ccgctcaccg cgcgcgtggc gcccaaggac gccttcacca cgtgttcgct
    15421 cttcgagccc cgctcgaccg tcgagcggga aaccggctcg gcccggcagt cgacggcgcg
    15481 gacggcgttc gacgatctct tcaagatctg aagaagccag aagccaggag ccggaatccg
    15541 gaattcagaa tccagaatcc cgacaagggc ggacaggaca cgcgggacag gcgcaggaga
    15601 cacggcagac acggcagaca cgacggacac gacggacacg gcggttgacg gaatggaggc
    15661 cggggcttca gccccggcgg gcggacaacc gccaggcagc agggacacac gatgatcacc
    15721 gagatcaccg ccgccgagct cgatcccgag cggttcgtcg ccgagcaggc cgcccggatc
    15781 cgggcggccg ttggcggcgg cacggccatc aacgcgctct cgggcggcgt cgattcgtcc
    15841 gtcgtgacga tgatcggcca ccgcgcgctc gggccgcgtc tccggacggt cttcgtcgac
    15901 aacggcctga tgcgcgaggg cgagccggag caggtcgtcg ggatgttccg ccgcctcggc
    15961 gtcgagatcg aggtcgtcga cgcccgcgcc gagttcctgg cggcgctggc gggcctcacg
    16021 gaccccgagc agaagcgcga ggcggtcacg cagaccttct acacgcgcgt gttcgggcgg
    16081 ctggtccgcg agagcggggc caggttcctg ctgcagggca cgatcctcac cgacgtggac
    16141 gagaccgtgg cgggcatcaa gcggcagcac aacgtcttcg agcagctcgg gatcgacccc
    16201 caggcggcct tcggctaccg gatcctggag ccgctcgtcc agctccgcaa ggacgccgtc
    16261 cgcacggtcg gccgctccct cggcctgccc gagtcgatgt tcgcgcgcat cccgtttccc
    16321 ggccccgcgc tggcggcccg gatcatcggc gaggtgacgg ccgagcggct cgccgcggtc
    16381 cggcgcgcca cctcgattgt cgagcgggcg ctcggcggcc tgggggcgtt ccagtacctg
    16441 gccgtcctcc acgaggaccg cgtcaccggc atgcgcgacg gccggcggga cttcggccag
    16501 cagatcgagg tccgctgctg ggacagcgtg gacgggcggc gcgccacgcc gacgcgcgtg
    16561 ccgttcgaga cgctggagtc gctggccgcc gagatcctgc gcgacgtgcc gggcgtggtc
    16621 agcgtgacct acaacatcgc cgccaagccg ccgtccacga tcgaggcggt gtagccgggc
    16681 gggccgctac cacccggcgg cgcggatgaa cgccaccgcg cggcggagct cgtccatcgg
    16741 gtcggtgccc gcgccgcgcg ccacgtagtt ctcgacaatc agcgcgccgg tgaacccggc
    16801 ccggcgcagg atctcgaaac acgccgccag ctcggcgcgg ccctctccgg gccgaacttc
    16861 ctgccccatc gccccgggag aggccggcag gagcgcgtcc ttgacgtgga ccgacgtgac
    16921 ccgcgacacg acgtgccgga gggcggccga cggggcgtcg acgccgtaga tgatcaggtt
    16981 cgcgggatcg aagttcacgc cgacgcgcgc gacggtgatc cgatcgagga acgccgcaag
    17041 ctgcgcgccg gtctcctggc cggtttcgag cgacacggtg atgcccttgg gcgcgccgta
    17101 gcgggcgatc tcggtgacgg gatccagcat cgcgcggt
//
LOCUS       DAOSIF010000012        16957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_3575_length_16957_cov_5.396935, whole genome shotgun sequence.
ACCESSION   DAOSIF010000012 DAOSIF010000000
VERSION     DAOSIF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16957
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3575_length_16957_cov_5.396935"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLT35_01025"
     CDS             complement(<1..1171)
                     /locus_tag="PLT35_01025"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOG27835.1"
                     /translation="MSLVSRRRGFGALALAAAVVFGPPSPAYGQGAYLDSSEEGRFQL
                     GSFSFTPAFFVTGGYDTNVTSEPGAIADYELVTVPQVEAWYRAGRLLLNGVSAAEFIN
                     YRVQRPIRTFNHFNGLSLRFPGAILEPRISASHRNHFARPTGFEIGERSRRIEDEIRG
                     SLSWSLSERTALLAEGRWLRINWDAAAQYRGSNLRESLNRTVGTGELGVSMALTPLTA
                     VSLSLQLGRDRFEFSPERDGNSLQAGAGVSFASPALISGSALVAYRRFDSPHSDSLDF
                     DGVVAAAHLAYIRETRTRFIVAVERQPYFSYAESLGYYLLNSASAKYVQTVLDNWEIA
                     AFGGISGLDYRRAGLPSHAGWDRTRHDFGGGLTCRIGAVTRIGVNASRTVSRGGLP"
     gene            1520..3946
                     /locus_tag="PLT35_01030"
     CDS             1520..3946
                     /locus_tag="PLT35_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27836.1"
                     /translation="MPVFGLYYRGRALLVRASDRFGAWCDAKTADAYALASPARQRAA
                     RAFFQWLGSLAHTAKLLPRLDAVRLVGPQWTAVWVGDPLLARELEVLLFPDDAERHPL
                     PRVAAWSLPRFARKCLDEADVVVCALPRRWPRFWRPSAPVAFTCPVFVNAVMDIRRPI
                     EEIVSGTRGDSLRRQLRIGGQAGLAVRLTASRTDWERFYLQMYRPHVERRHGDRALVS
                     PPDDHWREWIEPGGHLLLLEEDGSPLAGALVRIVGRVCFLGEEGVAQLPRAVEVGYGL
                     RALLRRSAVEYARSSGAAAFVMGRSLARTSDPVLASKLQWGPAIVPPERCLHPEWTFL
                     AAAVADPLRKHLNSQGLIAFTAGRAGVVSIGPPDKVQQHAASKVGHSLVVQAGSRNVL
                     VPTGAAPAEQEPGLPHAWGAGLYYRTRRRTVEIIDRLGLWCDLRTSDAFRLRSPRLRK
                     AVQGACQGAGRLAHVIKLLPRLDAVRLEAPGWSVVSVGDTNLDKELEHLLCPHGASKT
                     PLPRVAAWSLPRFVQEHLRVADLVVCSLPGAWPGVWRPRGPLVVRVPILVEAELDIAA
                     PVGEILRGSAREALRRQLRKSQEAGLQPRLTRERADLERFYREMYVPHVRARHGSRAF
                     VSAFSDHWERWIVPGGRLLLLERDGRPLVGVLLRVVGAVCFAGEEGVAQTDEARDHGY
                     GLRTALRVASIEYARSAGARRFVMGRSLSRGSSHVLMNKLQWGPEIVAARRCLQPEWT
                     FVASRLEGPLRERLNEHGLIALVSGRPCTVSFGEPTAAGFRHAVKTGSVLVVEAGRPH
                     RLIAAGAGPA"
     gene            complement(4010..4876)
                     /gene="rfbD"
                     /locus_tag="PLT35_01035"
     CDS             complement(4010..4876)
                     /gene="rfbD"
                     /locus_tag="PLT35_01035"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOG27837.1"
                     /translation="MRDASLLRRIFVAGSKGQLGAAFVSRLSREGEVTAVDLDELDLT
                     EPARVRDAVRSSGPSLILNCAAFNDVDGAEARPLEAYRVNAEAAWTLAGLARDLGAVL
                     VHYSSEFVFDGRLDRPYAEDDEPAPQSVYGATKLAGERFAAAAPKHYVLRLSSLYGGH
                     TGRTTVDWFVRQASAGGPVRAFADRTVSPSYVPDVVRATLDLVGRGAPFGLYHCGSPD
                     WCAWADIAGHILAACGRPELLEAVPFAHAPGRAARPRHCAMSSAKLGSVARAPRDWRT
                     ALDDYLAGRARA"
     gene            complement(4873..5892)
                     /gene="rfbB"
                     /locus_tag="PLT35_01040"
     CDS             complement(4873..5892)
                     /gene="rfbB"
                     /locus_tag="PLT35_01040"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOG27838.1"
                     /translation="MTKVLVTGGAGFIGSNFVRYALRQHPDWRVTTLDKLTYCGRLEN
                     LHDVADDPRHRFVKADIRDAAVSAPLVRESHIVVHFAAETHVDRSIQSADEFIQTDVL
                     GTFALLEAARGAKALRRFVQISTDEVYGSVPTGASKETDELLPRNPYSASKAAADRLA
                     YSYWATYKVPVVITRASNNYGPYQFPEKVIPLFITNALEDQGLPLYGDGLNVRDWLHV
                     EDHCRALDLVIEKGLDGQVYNIGGGNEVPNIDLTRRILALLGKPESLIRPVADRPGHD
                     RRYALDTTKLQALGWTPRCEFDAGLAATVEWYKANPWWWEPIKRQDPAYQAYYKAHYA
                     ERGPA"
     gene            complement(5889..6440)
                     /gene="rfbC"
                     /locus_tag="PLT35_01045"
     CDS             complement(5889..6440)
                     /gene="rfbC"
                     /locus_tag="PLT35_01045"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOG27839.1"
                     /translation="MNVVHTAIPGVLVFEPVVHGDDRGFFFESYREEAFRRATGCVRS
                     FVQDNHSRSVRNVLRGLHYQVRQPQGKLVRVVSGEVFDVVVDLRRSSPTFGRWVGTTL
                     SAQNKRQLWVPEGFAHGFLVTSDAAETIYKTTDYYAPQHERSLLWNDPDVGIAWPLEA
                     PPTLSAKDLAGKRLADCEVFDTL"
     gene            complement(6513..8315)
                     /locus_tag="PLT35_01050"
     CDS             complement(6513..8315)
                     /locus_tag="PLT35_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOG27840.1"
                     /translation="MHRLLSAVLAALVLAAPALAQTGANVLVVANEAHPDSVRIAEYY
                     AHSRSVPADQVLRIKAEVADEIERAAFNTQIQAPIANWLRQHAAQDRILYIVLAKGVP
                     LRVKGTPGRDGTAASVDSELTLLYQRLLGREPAVAGRIPNPYFLGAAPIAEAGPFSRA
                     ASELYLVVRLDGFTAADVVGLIDRGAAPSREGRFLLDQEAGPAESAGNRWLAAAAARL
                     REAGLGDRVVLEPTGKALTGEKGVLGYFSWGSNDAAITKRRLDLGFVPGAIAGTFVST
                     DGRTFTEPPAGWTIGRWTDRAGFFAGSPQSLAGDLIREGVTGIAAHVDEPFLDATIRP
                     DILFPAYAAGFNLAESFYLAMPYVSWRTVVVGDPLCAPFPRQAVPSSDIDAGLDSASG
                     LPARFAERRLQVLTSAGTRPDAARAVLRAEALASGGDKAGARKALEEATALDATLVGA
                     HLQLAGEYEAGQDTDKAIERYRLVLAEQAGNVLALNNLAYALAVRKGQAAEALPHAER
                     AYRLSGGRSPEIADTYAWVLHLLGRDAEALPIQEQAARSASSSAMHRLHLSAIYAALG
                     RVDEAAAELKEAVRLQPDLESGDEVKALRAKIGG"
     gene            8614..8865
                     /locus_tag="PLT35_01055"
     CDS             8614..8865
                     /locus_tag="PLT35_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOG27841.1"
                     /translation="MNSRAVSMRELQQNLKKVVARVERGETVQLTKRRRVVAKLVPVW
                     PQKPAESWPDLDARARAIFGDRMITPSPSEELIKERGEW"
     gene            8859..9308
                     /locus_tag="PLT35_01060"
     CDS             8859..9308
                     /locus_tag="PLT35_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOG27842.1"
                     /translation="MVNAYFDSSVLVAFYLVESHSYAARRAVTGTPRVLFTPLHQLEV
                     RNTFRVQVGRERMTSEESEGVLRLLEEDVAAGRLAPTPLDLYAVFERAEGLSAKHTRQ
                     LLSRSLDVLHVAAALELGCTRFVSFDARQVKLAAAAGLRAAVPPPAA"
     gene            complement(9376..10548)
                     /locus_tag="PLT35_01065"
     CDS             complement(9376..10548)
                     /locus_tag="PLT35_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOG27843.1"
                     /translation="MSRRFVLALAAASMLALSAVPSDAIPAFARKYQYSCSTCHQPAP
                     RLKPFGDDFAARGFRLEDRSQEPPRATYDVGDPTLKLMRELPLAIRLDGYATFGEKNS
                     RGADVQYPWVFKLLSGGQINQKISYYFYYIIEKGGEAGLEDAWVQINNPFKLPIDVAV
                     GQFQVCDPMFKRELKLERYDYQIFKTHVGTAGVDLTYDRGLIVSWDFPGGVESVLQVV
                     NGNGIGMADDFGNFDRDNLKNFAYRLAKPLDKVRLGLFGYYGREQGAGGARNRTFYFG
                     PDVVVDVNDRFQLNLEYLERRDDNPTFLPSRRGADWKTRGGFAELVYMPLGADGPWSV
                     AGLYNKVASDDPNAKYQSLSLNVGRLLARNVRLTADIGREFEREGTKFSLGLVTVF"
     gene            complement(10584..11954)
                     /locus_tag="PLT35_01070"
     CDS             complement(10584..11954)
                     /locus_tag="PLT35_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOG27844.1"
                     /translation="MSPRSSVFVPALAALLLAGAGFAATQAPPKAGDAALGAQKYDAY
                     ERPAACATCHKDIARQHEQAMMSQAYTHHWDEIEYFGLAVPHAKKDPKFKGAADGCNG
                     CHTPFAFLAGDVPPPLPSANSRANESVSCDLCHTVTGFEGDTPFNFNWISVPGKVKLG
                     TREGVVSPHHVTKKNDFLKTAEFCGTCHNEKDPFGLWVKGTHLEWKAGPHGKAGIVCQ
                     DCHMPPSAGRSATMGSELPDLRQHLFHGAHDPGKLSGVIEVRIHPEAREAEPGETMKL
                     TAVFVNAKAGHSVPSGSAEERVLWAHVTARDAKGQVYHLPVDKKGFPGEEYTIASDAL
                     AYQDIGEVKDIANFAGLKRDGEVPAGDRIFRLPYLDPQGRMTIMQWNTASFGPDYRLA
                     PLSARAETYTWRLPATLPAGPVVVTAEVYYSRLVSSVAEFMKVPREESEPVKVNSHST
                     AFTILP"
     gene            12179..12724
                     /locus_tag="PLT35_01075"
     CDS             12179..12724
                     /locus_tag="PLT35_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG27845.1"
                     /translation="MLAAPDCGAGLGDVIARGLGLFNVDVRGCRVLLKPNLVEYEPGT
                     MIDTHPAALISAAEAFLPVGAREVVVGEGPGHQRGAECLLTASGLSDCLRELRIPFVD
                     LNKDAVRPASLRSRFMGRDARLLSVDVLRSDLVVSVARLRTHHWTGMTASVKKLFGVV
                     PGAVYYSARGTARVDRPVPIR"
     gene            12749..13942
                     /locus_tag="PLT35_01080"
     CDS             12749..13942
                     /locus_tag="PLT35_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG27846.1"
                     /translation="MLPSRALRPAQEAPLAPARPLIVGRSAALLAICELAERVAAGDA
                     KVLITGESGVGKDLVARLIHFHSSRAAQPYIAVNCAGVPETLLESQIFGHVKGAFTDA
                     YRDKAGILQQAHNGTVFLDEVAEMSLRMQAMLLRFLENGEIQPVGSDLVKTQVDVRVI
                     AATNKDLATAVAAGHFREDLMYRLDVVHIRVPALRERPEDIRALVEHQLARAERRITL
                     TDEAIRALERYRWPGNVRELQNVIERLIWMSATDTVGIEDLPVRLLPGRTERVFPARE
                     RRRQRADELFKALTDGSFSFWDHIHPLFLDRDLTRHDLRELVRRGLAATRGNYRAVVR
                     LFGMPDADYKRFLNFLGTHGCVVDFREFRTPAAADGAVVKDRVAREAGPPKGGGAAHP
                     TGSGL"
     gene            13939..15696
                     /locus_tag="PLT35_01085"
     CDS             13939..15696
                     /locus_tag="PLT35_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOG27847.1"
                     /translation="MSKRTFLGPLIGRGPGQCELVNALSGSRLAARVEPAFDSASRRR
                     GLLGRDALPPDAVLVLAPCSAIHTISMRFPIDVIFVARNGTVLKTRSNLPPWRIAWAW
                     RAHAVIEAAAGFLERTEIVPGEVVAVREIDGARVPAAEQDASPASPARTPAGPVARAK
                     TRTTGRPGRVTLADLIAHAVPRAWFESVAIVQELCAVALARGPAHDLRVPELRHVAIT
                     PTGEVELLAEGPAGHSPVHRASLVLLALTPEAELPVQLKLLALEELSPRPKLGNLPDL
                     HKELEFFERPDRRAIIQGVYERYRAQTAPSKGLAAVPTPLLEPSQPRRRWRWWRSRGL
                     WISVAVASLAIAVALAVRAAPRPDGQRMRLAVSDLSRAASNAGRFVAAKASEQVRVLK
                     WRLGLNKRPPRRLPDIRASSPAPAPAEAAVATPPLGPAEADARLPAAVQAPGEPGVPE
                     GAPAPVPVAPTAAAPPSASVPHRAAEAGAGDPSSGAALPASAVVYTASDPLVVPPVLV
                     RPTLPAGPPPGMRTEELPEVEIVVSAAGDVSSVRLVSAGAGARQAMMLSAVKAWQFRP
                     ATRGGQPVPYRLRLRLPAN"
     gene            15907..16215
                     /locus_tag="PLT35_01090"
     CDS             15907..16215
                     /locus_tag="PLT35_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27848.1"
                     /translation="MLGRRRHSRYLLAQPVEGHLRVREEVTIKKWSGSDVVVLASEPC
                     ACNERLTLEVPGPLGRRLHVQVVECRPAVTDEGAILHRCRLAILEAAADCAGPAASEP
                     "
     gene            16212..16658
                     /locus_tag="PLT35_01095"
     CDS             16212..16658
                     /locus_tag="PLT35_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27849.1"
                     /translation="MSVERSRGPSQASAIPVGTLVRSVPVRLVEVSSGGCLLESDRRL
                     KAGTCGLLAVPLAGLVHADDVRVARCQQVAGAGAAFNVGAELLRTRRFGRRSLRAAVA
                     RIVGDGRGVGHARPDAEEPQPVGVRTDGAGGRCESRAPPAHSDNGP"
     gene            16745..16909
                     /locus_tag="PLT35_01100"
     CDS             16745..16909
                     /locus_tag="PLT35_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOG27850.1"
                     /translation="MKNLIRRFIRDEEGQDIIEYALLAAFISIIAIAAVRAVGGRVQV
                     YFGNVQSALS"
BASE COUNT         2454 a         5800 c         6103 g         2600 t
ORIGIN      
        1 aggggaggcc gcctcgagag accgtgcgcg acgcgttcac gccaatccgg gtgaccgcgc
       61 cgatgcggca cgtcaggccg ccgccgaagt cgtgccgcgt gcggtcccag ccggcgtgtg
      121 acgggagccc ggcccggcgg tagtcgaggc cgctgatgcc gccgaatgcg gcgatctccc
      181 agttgtcgag caccgtttgc acgtacttgg cgcttgccga gttcaggaga tagtacccca
      241 ggctttcggc gtacgaaaag tacggctgcc gctcgacggc gacaatgaac cgggtgcgcg
      301 tctcccggat gtaggccaga tgggccgccg ccacgacgcc gtcgaaatcc agggagtccg
      361 agtgcggaga atcgaaccgg cggtacgcga ccagcgcgga ccccgagatg agggccgggc
      421 tggcgaagga cacgcccgcg ccggcctgga ggctgttgcc gtcccgctcc gggctgaact
      481 cgaaccggtc gcggcccagc tgcagcgaca gcgaaaccgc cgtcaacgga gtcagggcca
      541 tcgacacgcc cagctcgccc gtgccgacgg tgcggttcag cgattcgcgc aggttcgagc
      601 cgcggtactg cgccgccgcg tcccagttga tccgcagcca tcggccttcg gccaacaggg
      661 ccgtgcgctc gctgagcgac caggacaggc tcccgcgaat ctcgtcctcg atccggcgcg
      721 agcgctcgcc gatctcgaag ccggtcgggc gggcgaaatg gttccggtgg gaggcgctga
      781 tcctcggctc gaggatggcg ccgggaaagc ggagcgacag gccgttgaaa tggttgaacg
      841 tgcggatcgg ccgctgcacg cggtagttga tgaactcggc ggcgctgacg ccgttcagca
      901 gcagcctgcc cgcccggtac caggcttcga cttgcgggac ggtcacgagc tcgtagtcgg
      961 cgatcgcgcc gggctcgctc gtgacattcg tgtcgtaccc gcccgtgacg aaaaacgcgg
     1021 gtgtgaacga gaagctcccg agctggaacc tgccttcctc cgacgagtcg aggtacgccc
     1081 cctggccgta cgccggagac ggagggccga acactaccgc agccgcaagg gcgagcgcgc
     1141 cgaaaccgcg gcgccgggag acaaggctca ctcgggcgcg ttgatgcatg aaacgagcca
     1201 ggctggggag aaacgcccgg actgttcgat ctcgttcatg gagcggcatt tcattcctgc
     1261 agcggcggga tcctctccga taaccccgag cttgtaccga gttgcggtga ctattgtctt
     1321 cccgatcggg acatgtgccg acagcacaag ccaccacgcg aagtaatctc gaaatccgct
     1381 ggaagcccag ccgaggcgcc tcgcggggag tctgctagag ttctttcggg cgaaatgcca
     1441 ggctcgttca gcccaggacc ggcgcccaac cctgggggat ccgggctggc aggcgccttg
     1501 cgacgactgg ttccgagaga tgcccgtgtt cggactctat taccgcggcc gcgccctcct
     1561 cgtccgagcc tccgaccgct tcggcgcctg gtgcgacgcg aaaaccgcag acgcctacgc
     1621 cctcgcgtct cccgcgcgcc aacgcgcggc gcgggcgttc ttccagtggc tcggaagcct
     1681 ggcgcacacc gccaagctcc tgccaaggct cgacgccgtc aggctggtag ggccccaatg
     1741 gaccgcggtc tgggtgggcg acccgctcct ggcgcgggaa ctggaggtcc tgctcttccc
     1801 cgacgacgcg gagcggcacc cgctgccgcg ggtggcggca tggtcgcttc cgcggtttgc
     1861 gcgaaagtgc ctggacgaag cggatgtcgt cgtctgcgcc ctgccgcgcc ggtggccccg
     1921 gttctggcgg ccttccgcgc ccgtggcgtt cacgtgtccg gtcttcgtga acgcggtcat
     1981 ggacatccgg aggccgatcg aggagatcgt cagcggcacc cgcggcgact cgctcaggcg
     2041 gcaactgcgg atcgggggac aggctggatt ggccgtccgc ctgaccgcgt cgcgaaccga
     2101 ctgggaacgc ttctacctgc agatgtaccg cccgcacgtg gagcggcggc acggcgacag
     2161 ggcgctggtc agcccgccgg acgatcactg gcgggagtgg attgaacccg gcggccacct
     2221 gctcctcctc gaagaagacg gcagcccgct ggccggcgcc ctcgttcgaa tcgtcggccg
     2281 cgtgtgcttc ctcggggaag agggcgtcgc tcagctgccc agggccgtcg aggtgggata
     2341 cgggctgcgc gccctgctca ggcgctccgc cgtcgagtac gcgcgctcgt ccggcgcggc
     2401 ggccttcgtc atgggccgga gcctcgcccg aaccagcgac cccgtgctgg cgagcaagct
     2461 ccagtggggg ccggcgatcg tgccgcccga acgctgcctg catccggagt ggacgttcct
     2521 ggcggcggcg gtcgcggatc cgctgcgtaa gcatctgaac agccagggct tgatcgcgtt
     2581 cacggcgggg cgagccggcg tcgtgtcgat cggcccgccg gacaaggtgc agcagcacgc
     2641 cgcgtcgaag gtggggcact cgctggtcgt ccaggccggc tcgaggaacg tgctggttcc
     2701 gactggcgct gccccggcgg agcaggagcc tggattgccg cacgcctggg gcgcggggct
     2761 ctactacagg acgcgccggc ggacggtcga gatcatcgac cgcctgggcc tgtggtgcga
     2821 tctccgcacg tcggatgcgt tccggctgcg aagtccccgg ctcagaaagg cggttcaggg
     2881 cgcatgccag ggggcgggaa gactcgcgca cgtcatcaag ctcctgcccc ggctggacgc
     2941 cgtgcggctg gaggcgccgg gctggagcgt ggtcagcgtc ggcgacacga acctcgacaa
     3001 ggagctggag cacctgctgt gcccgcacgg cgcgtcgaag acgccgctgc ccagagtggc
     3061 ggcgtggagc cttccgcgat tcgtgcagga gcacctccgt gtggcagacc tcgtggtctg
     3121 ctccctcccg ggagcgtggc ccggcgtctg gcgcccccgc ggcccgcttg tcgtcagagt
     3181 accgattctc gtcgaggccg agctggacat tgcggcgccg gtcggagaga tcctgcgggg
     3241 ctcggcgcga gaggcgctcc ggcggcagct caggaagagc caggaggccg gcttgcagcc
     3301 gcggctcacc cgcgaacggg ccgatctgga gcgcttctac cgcgagatgt acgtgccgca
     3361 cgtccgcgcg cggcacggct ctcgcgcctt cgtcagcgcc ttcagcgacc actgggagcg
     3421 ttggatcgtg cctggcggcc gcctgctgct cctggaacgg gacggaagac cgctcgtcgg
     3481 cgtcctcctc cgcgttgtcg gcgcggtctg cttcgccgga gaagagggag ttgcgcagac
     3541 tgatgaggcc agggatcacg gatacgggct ccggacggcg ttgagggtcg cctcaatcga
     3601 gtacgcgcgc tccgccggcg cgaggcgctt cgtcatgggg cgcagcctgt cgaggggcag
     3661 cagtcacgtg ctgatgaaca agctgcagtg ggggccggag atcgtggcgg cgcggcggtg
     3721 cctgcagccc gaatggacgt tcgtggcgtc gcgcctggag ggcccgctgc gcgagcgcct
     3781 caacgagcac gggctgatcg cgctcgtctc cgggcggccc tgcacggtgt cgttcggcga
     3841 gccgaccgcg gccgggttcc gccacgccgt gaagacgggg tcggtgctcg tcgtggaggc
     3901 cggccggccc cacaggctga tcgctgccgg cgccgggccg gcgtaaggcg tcccggagct
     3961 ttccggagcg cggggcgagg gacgagacgg agccggagcg cccgccccat caggcccgcg
     4021 cgcgccccgc gaggtagtcg tcgagggcgg tgcgccagtc cctcggcgcg cgcgcaacgg
     4081 agccgagctt cgcgctcgac atggcgcagt gccgcggacg cgcggcccgg ccgggggcgt
     4141 gcgcgaaggg caccgcctcc agcagctcgg gacggccgca agcggccagg atgtggccgg
     4201 cgatgtcggc ccacgcgcac cagtccggcg atccgcagtg gtagaggccg aatggcgcgc
     4261 cccgcccgac gagatcgagc gtcgccctga cgacgtccgg gacgtagctg ggggagaccg
     4321 tgcgatcggc gaacgccctg acgggcccgc cggccgaggc ctgccgcacg aaccaatcga
     4381 cggtcgtcct gcccgtgtgc ccgccgtaga ggctcgacag gcgcagcacg tagtgcttcg
     4441 gcgccgcggc ggcgaagcgc tcgccggcga gcttcgtcgc gccgtacacg gactgcggcg
     4501 cgggctcgtc gtcttcggcg tacgggcgat ccagccgccc gtcgaagacg aactcgctcg
     4561 agtagtgcac cagcacggcg cccagatcgc gcgcgaggcc ggccagggtc caggcggcct
     4621 cggcgttcac gcggtacgcc tccagcgggc gcgcctcggc gccatcgacg tcgttgaacg
     4681 cggcgcagtt cagaatcagc gacggccccg acgaccgcac cgcgtcgcgc acgcgagcgg
     4741 gctcggtgag gtcgagctcg tccagatcga cggcggtgac ctcgccttca cgggagagcc
     4801 gcgacacgaa ggcggcaccg agctggccct tcgacccggc gacgaagatg cgccgcagaa
     4861 gcgaggcgtc cctcacgcag gacctcgctc ggcgtaatgg gccttgtagt acgcctggta
     4921 ggcgggatcc tggcgcttga tcggctccca ccaccacggg tttgccttgt accactcgac
     4981 ggtggcggcc agccccgcgt cgaactcgca gcgcggcgtc cagccgagcg cctgcagctt
     5041 cgtcgtgtcc agggcatagc ggcggtcgtg gccggggcgg tcggccacgg ggcggatgag
     5101 cgactcgggc tttcccagca gcgcgaggat gcgccgcgtc aagtcgatgt tcggcacctc
     5161 gttgccgccg ccgatgttgt acacctggcc gtccaggccc ttctcgatca cgaggtcgag
     5221 ggcgcggcag tgatcctcga cgtgcagcca gtcgcgcacg ttcaggccgt cgccgtagag
     5281 cggaagcccc tgatcctcga gggcgttcgt gatgaagagc ggaatcacct tctcggggaa
     5341 ctggtacggc ccgtagttgt tcgacgcgcg cgtgatgacg acgggcacct tgtaagtggc
     5401 ccagtagctg taggccagcc gatcggcggc ggccttgctc gccgagtacg ggttgcgcgg
     5461 caggagctcg tccgtttcct tcgatgcgcc ggtcggcacc gagccgtaga cctcgtcggt
     5521 cgagatctgg acgaaccgcc gcagggcctt cgccccccgc gccgcctcga gcagggcgaa
     5581 ggtgccgagg acgtcggtct ggatgaactc gtcggcgctc tgaatcgacc ggtccacgtg
     5641 cgtctcggcc gcgaagtgca cgacgatgtg cgactcgcgc acgagcggcg cgctgacggc
     5701 ggcgtcgcgg atgtcggcct tcacgaagcg gtgccgcggg tcgtcggcga cgtcgtgcaa
     5761 gttctccagc cgcccgcagt aagtcagctt gtccagcgtg gtgacgcgcc agtcggggtg
     5821 ctggcgcagg gcatagcgga cgaagttgct gccgatgaac ccggcgcctc cggtgacgag
     5881 taccttggtc atagggtgtc gaagacctcg cagtcggcca gccgcttccc ggcgagatcc
     5941 ttggccgaga gcgtcggcgg cgcctcgagc ggccacgcga tgcccacgtc gggatcgttc
     6001 cagagcagcg agcgctcgtg ttgtggggcg tagtagtcgg tcgtcttgta gatggtttcc
     6061 gcggcgtccg acgtcacgag gaagccgtgg gcgaatcctt cgggcaccca gagctgccgc
     6121 ttgttctggg cggagagcgt cgtgccgacc cacctgccga acgtcggcga gctgcggcgc
     6181 agatccacga cgacatcgaa gacctcgccc gagacaacgc ggacgagctt cccctgcggc
     6241 tggcggacct ggtagtggag cccgcgcagg acgttgcgca ccgagcgcga gtgattgtcc
     6301 tggacgaacg agcggacgca tcccgtcgcc cgccggaacg cctcctcccg gtagctctcg
     6361 aagaagaacc cgcggtcgtc cccgtgcacg accggctcga agacgagcac gccgggaatc
     6421 gccgtgtgaa cgacgttcat ctgcaccgtt cctctcgcgc tgcctcggcc gccagccggt
     6481 cgcgcttcaa ccgcagcgtc cggccgcttc catcatccgc cgattttcgc ccgcagggcc
     6541 ttgacctcgt ctccgctctc gaggtcgggc tggagccgga cggcttcctt cagctcggcc
     6601 gccgcctcgt cgacgcggcc gagcgcggcg tagatcgccg agaggtgcag ccggtgcatg
     6661 gcgctcgacg aggcggacct ggccgcctgc tcctggatgg gcagggcctc ggcgtcgcgt
     6721 cccaggagat gcagcaccca ggcgtatgta tccgcgatct ccgggctccg tccgcccgag
     6781 agcctgtacg cccgttccgc gtggggaagc gcttcggcgg cctggccctt ccgcacggcc
     6841 agcgcgtagg cgaggttgtt gagcgccagc acgttgcccg cctgctcggc cagcacgagc
     6901 cggtagcgct cgatggcctt gtcggtgtcc tggccggcct cgtattcacc cgcaagctga
     6961 aggtgcgccc cgacgagcgt cgcatcgagg gccgtcgcct cttcgagcgc cttccgagcc
     7021 ccggccttgt cgccgccgga ggcgagcgcc tccgcccgca ggaccgcccg ggccgcgtcg
     7081 gggcgcgtgc cggccgatgt cagcacctgc aggcgccgct cggcaaaccg cgcgggcagc
     7141 cccgacgccg agtcgaggcc cgcgtcgatg tcagacgacg gcaccgcctg gcgcgggaac
     7201 ggcgcgcaga ggggatcgcc aaccaccacg gtccgccagc tcacgtacgg catcgccagg
     7261 tagaacgact cggccaggtt gaagccggcc gcgtaggccg gaaagaggat gtcgggacgg
     7321 atcgtggcgt ccaggaacgg ctcgtccacg tgagcggcaa tgccggtcac gccttcccgg
     7381 atgagatcgc cggcgagcga ctgcggagag ccggcaaaga agccggcccg gtcggtccac
     7441 ctgccgatcg tccatccggc gggcggctcc gtgaacgtgc ggccgtccgt gctgacgaac
     7501 gtgccggcaa tggcgccggg cacaaagccg aggtcgagcc ggcgcttggt gatcgccgcg
     7561 tcgttcgatc cccaggagaa atagccaagg acgccctttt cgcccgtcag ggccttgccg
     7621 gtgggctcga gcacgacgcg atcccccagg cccgcctcgc gaaggcgcgc ggccgcggcg
     7681 gccagccacc ggttgccggc gctctccgct ggcccggcct cctggtccag caggaagcgg
     7741 ccctcgcgcg acggagctgc cccccggtcg atgagcccga cgacatccgc cgccgtgaag
     7801 ccgtcgaggc ggacgacgag gtacagctcg gaagccgcgc ggctgaacgg tcctgcctcc
     7861 gcgatcggcg ccgcgccgag gaagtacgga ttggggatgc gcccggccac cgccggctcg
     7921 cgtccaagca gccgctggta gagcagcgtc aactccgagt ccacgctcgc ggccgtgccg
     7981 tcgcggccgg gggtgccttt gacccggagc gggacgcctt tcgccaggac gatgtagagg
     8041 atgcggtcct gggcggcgtg ctggcgcagc cagttggcga tgggcgcctg gatctgggtg
     8101 ttgaatgccg cccgctcgat ctcgtctgcg acctccgcct tgatgcggag cacctggtcg
     8161 gccggcacgc tgcggctgtg ggcgtagtac tcggcgatcc ggaccgaatc cgggtgcgcc
     8221 tcgttggcga ccacgagcac gttggcgccg gtctgggcga gagccggcgc cgcgaggacc
     8281 agcgccgcga ggaccgcgga cagcaagcga tgcatcgtta cgattatcga cggttttcac
     8341 cgcgcgcgtg agagcccgcc tcgaccagga gcggccgcgc ccgcgcggag cggtcgtcgc
     8401 cgatgcgagg gtggcgccgg cgcgtctcga gcccgtccgc ggaatgtgcg acgtgcccgg
     8461 gtcccggacg cggcaattcc tgccgccagc tgccggcggg atcaagtttc cggctgggaa
     8521 accgggcttc gtttcgcggt gggtcgccgg cacgggccgt gcacacatgg aaggccgttg
     8581 acagaggcca gcacgtacta catcatgtag tacatgaatt ccagggccgt ttccatgcgg
     8641 gaactccagc agaacctgaa gaaggtggtc gcccgcgtcg agcgcggcga gaccgtccag
     8701 ttgacgaagc gccggcgcgt cgtggccaag ctggtgccgg tctggccgca gaagcccgcc
     8761 gagtcgtggc ccgacctcga cgcgcgcgca cgggcaatct tcggagaccg gatgatcacg
     8821 ccgtcgccct ccgaggagtt gatcaaggag cgcggcgagt ggtgaacgcc tacttcgact
     8881 cgagcgtgct tgtggcgttc tacctcgtcg aatcgcactc gtacgcggcg cgccgggcgg
     8941 tgaccggcac gccgcgcgtg ctgttcacgc cgctccacca actcgaggtg cggaacacct
     9001 tccgggtgca ggtgggccgg gagcgaatga ccagcgagga atccgagggg gtgcttcggc
     9061 ttctggagga agacgtcgcc gccgggcggc tcgcgccgac gccgctggat ctctacgcgg
     9121 tcttcgagcg ggcggagggg ctgtccgcca agcacacgag acagctgctc tcacgcagcc
     9181 tcgacgtcct gcacgtggcc gcggcgctcg aactgggctg cacgcgcttc gtgtcgttcg
     9241 atgcccgcca ggtgaagctc gccgcggccg ccggcctgcg cgccgccgtc cccccgccgg
     9301 cagcgtgacc ccgccagcgc agcgagcctt gccccttgtc ccggtctgtc tggcgcctgg
     9361 cgcccggccc catgcctaga acaccgtaac gagccccagg ctgaacttgg tgccctcgcg
     9421 ctcgaactcg cggccgatgt ccgccgtcag ccgcacgttg cgcgcgagca ggcgccccac
     9481 gttgagcgac aggctctggt acttcgcgtt cggatcgtcg gaggcgacct tgttgtacag
     9541 gccagccacc gaccacggcc cgtccgcccc gagcggcatg tagaccagtt ccgcgaaccc
     9601 gccgcgcgtc ttccagtcgg ccccgcgcct gctcggcagg aacgtcggat tgtcgtcgcg
     9661 ccgctccagg tattccaggt tcagctgaaa gcgatcgttg acgtccacca ccacgtcggg
     9721 gccgaaatag aacgtgcggt tccgggcgcc gccggcccct tgctcgcgcc cgtagtagcc
     9781 gaacaggccg agcctgacct tgtcgagggg ctttgccagg cggtacgcga agttcttcag
     9841 gttgtcccgg tcgaagttgc cgaaatcgtc ggccatgccg atgccgttgc cgttcacgac
     9901 ctgcagcacc gactcgaccc cgccggggaa gtcccagctg acgatgaggc cgcggtcgta
     9961 ggtgaggtcc acgccggccg tgccgacgtg ggtcttgaag atctggtagt cgtagcgctc
    10021 gagcttgagc tcgcgcttga acatcgggtc gcagacctgg aactgcccga ccgcgacgtc
    10081 gatgggcagc ttgaaggggt tgttgatctg cacccacgcg tcctcgaggc cagcctcgcc
    10141 gcccttctcg atgatgtagt agaagtagta cgagatcttc tggttgatct gcccgcccga
    10201 cagcagtttg aacacccacg ggtactgcac gtcggcgccg cggctgttct tctcgccgaa
    10261 ggtcgcgtag ccgtccaggc ggatggccag cggcagctcg cgcatgagct tcagcgtcgg
    10321 gtcgcccacg tcgtacgtcg cccggggcgg ctcctggctc cggtcctcga gcctgaaccc
    10381 gcgcgccgcg aagtcgtcgc cgaacggctt caggcgcggc gcgggctggt ggcacgtcga
    10441 gcacgaatac tgatacttgc gggcgaacgc cggaatggcg tccgagggaa cggcggagag
    10501 cgccagcatc gacgccgccg cgagcgcgag cacgaatcgt cgggacatat cgggcctcac
    10561 ggaactcatg cggatcaggt ccgtcacgga agaatggtaa acgccgtcga gtggctgttc
    10621 accttcacgg gctccgattc ctcgcgcggc actttcatga actcggccac cgacgacacc
    10681 aggcgcgagt agtacacctc ggccgtgacg accaccgggc ctgccggcag cgtggccggc
    10741 agccgccacg tgtaggtctc cgcgcgggcc gagagcgggg cgaggcggta gtcggggccg
    10801 aagctcgccg tgttccactg catgatggtc atgcggccct gcggatcgag gtagggcagg
    10861 cggaagatgc ggtccccggc cgggacctcg ccatcccgct tcaggcccgc gaagttcgcg
    10921 atgtccttca cctcgccgat gtcctgatac gccagggcgt ccgaggcgat cgtgtactcc
    10981 tcgccgggga accccttctt gtccacgggc agatggtaga cttggccctt cgcgtccctc
    11041 gccgtcacgt gcgcccagag cacgcgctcc tcggccgatc cggacggcac gctgtggccg
    11101 gccttcgcgt tcacgaacac ggccgtcagc ttcatcgtct cgccgggctc ggcctcgcgc
    11161 gcttcggggt ggatgcgcac ctcgatgacg ccggagagct tgccggggtc gtgcgcgccg
    11221 tggaacaggt gctgccgcag gtccggcagc tccgacccca tcgtcgcgct ccggcccgca
    11281 gacggcggca tgtggcagtc ctggcagacg atgcccgcct tgccgtgggg gccggccttc
    11341 cactcgaggt gggtgccctt cacccacagg ccgaaggggt ccttctcgtt gtggcaggtg
    11401 ccgcagaact cggccgtctt gaggaagtcg ttcttcttcg tcacgtggtg cgggctgacc
    11461 acgccttcgc gcgtcccgag cttgaccttg cccggcaccg agatccagtt gaagttgaac
    11521 ggcgtgtcgc cctcgaagcc cgtcaccgtg tggcagaggt cgcacgacac ggattcgttg
    11581 gcgcggctgt tggccgacgg cagcggcggc ggcacgtcgc ccgccaggaa cgcgaacggc
    11641 gtatggcagc cgttgcaccc gtcggccgcg cccttgaact tcgggtcctt ctttgcgtgc
    11701 gggacggcga ggccgaagta ctcgatctcg tcccagtggt gcgtgtaggc ctgcgacatc
    11761 atcgcctgct cgtgctggcg ggcgatgtcc ttgtggcagg tggcgcaggc ggcggggcgc
    11821 tcgtaggcgt cgtacttctg cgcgccgaga gccgcgtcgc cggccttcgg cggggcctgc
    11881 gtagcggcga agcccgcgcc ggccaggagc agggccgcca gcgcggggac gaacacggag
    11941 cttctcgggg acatgacgcc tccagtcgcc ctgcattgtt gagtacgcgt gacgaaaagg
    12001 gcaagcagga ccaaggcgag cgtagttgcg cggccggtgc gacggcgtct ctggtggagg
    12061 ccggaccttc aggtccggcg tgtcgttgtg gcggcgaaga gccgctcgat ttcccgcctg
    12121 cacgcgccag aagtggaacc gagccctctt cgccgtacct gagcgctcga gtggcgcggt
    12181 gctcgcggcg ccggactgcg gcgccggcct aggcgatgtc atcgcgcgcg gactcgggct
    12241 cttcaacgtc gacgtgcggg ggtgccgggt gctgctgaaa ccgaacctcg tcgagtacga
    12301 gccgggtacg atgatcgaca cgcatccggc ggccttgatc agcgcggccg aggcattcct
    12361 cccggtgggt gcccgcgaag tcgtcgtggg cgaagggccc ggccaccagc gcggcgcgga
    12421 gtgtctcctc acggcaagcg gcctgtcgga ctgcctgagg gagcttcgga ttccgttcgt
    12481 tgatctgaac aaggacgccg tgcggccggc gagcctccgg agccggttca tgggccgcga
    12541 cgcccggctg ctgtccgtgg atgtgctgcg gtccgatctc gtcgtatcgg tggccaggct
    12601 caggacccat cactggaccg gcatgacggc gagcgtgaag aagctgttcg gcgtcgtccc
    12661 gggcgctgtc tattactccg cgcgtgggac cgcgcgtgtg gatcggccgg tcccgattcg
    12721 atagaatagc ctccacttgg gacatcccat gctgccgagc cgggccttgc gccctgccca
    12781 ggaggcgcct ctggcgcctg ccaggccgct catcgtcggc cggagcgcgg cgttgctcgc
    12841 catctgcgag ctggccgagc gcgtggccgc cggcgatgcc aaggttctca ttaccggtga
    12901 gagcggcgtc ggcaaggacc tcgtggcccg cctgatccac ttccactcga gccgggcggc
    12961 gcagccctac atcgcggtga actgtgcggg ggtgccggag accctgctcg agtcgcagat
    13021 attcggccac gtcaagggcg cgttcaccga cgcctacagg gacaaagcgg gcatcctcca
    13081 gcaggctcat aacggcacgg tcttcctgga cgaggtcgcg gagatgagcc tgcggatgca
    13141 ggcgatgctc ctgcggttcc tcgagaacgg ggagatccag cccgtcgggt ccgacctggt
    13201 gaagacgcag gtcgacgtgc gcgtcatcgc ggccacgaac aaggacctgg ccaccgcggt
    13261 cgcggcaggt cacttccgcg aggacctgat gtaccgcctc gacgtcgtgc acatccgcgt
    13321 gcccgcgctc cgggagcggc ccgaggacat tcgcgccctc gtcgaacacc agctcgctcg
    13381 ggccgagcgg aggatcacgc tgaccgacga ggcgatccga gccctcgagc ggtaccgctg
    13441 gcccggcaac gtccgcgagc tgcagaacgt catcgagcgc ctcatctgga tgtccgccac
    13501 ggacactgtc ggcatcgagg acctgccggt gcgcctgctg cccggcagga ccgagcgggt
    13561 gtttcccgcc agggagaggc gccggcagcg ggccgacgag ctgttcaagg cgcttaccga
    13621 cggctccttt tcgttctggg accacatcca ccccctcttt ctcgaccggg acctgacccg
    13681 gcacgacctg cgcgagctcg tccgcagggg cttggccgcc acccggggca actaccgggc
    13741 ggtcgtgcgg ctgttcggca tgccggacgc cgattacaag cgcttcttga acttccttgg
    13801 cacccacggc tgcgtcgtgg acttccgcga gttccggacc cccgcggcag cggatggggc
    13861 ggtcgtaaag gatcgtgttg cgcgggaggc cggtcctccg aaaggcggcg gggcggctca
    13921 cccgacggga agcggcctat gagcaagcga accttcctcg gccccctgat cgggagaggc
    13981 cccggccagt gcgaactcgt gaacgctctc agcggctcgc ggctggcggc gcgcgtcgag
    14041 ccggctttcg actcggcgtc ccgcagacgg gggctgctcg gccgcgacgc gctgccgccg
    14101 gatgcggtgc tcgtcctggc gccgtgcagc gcgatccaca ccatctcgat gcgcttcccg
    14161 attgacgtga tcttcgtcgc gcggaacggg acggtgttga agacgcgttc gaacctgccg
    14221 ccctggcgca tcgcctgggc ctggcgcgcc cacgccgtca tcgaggcggc ggccgggttc
    14281 ctcgagcgca ctgaaatcgt gccgggcgaa gtggtcgccg tgcgcgagat tgacggcgcg
    14341 cgcgtccccg cggcggagca ggatgcctcg cccgcgtcgc cggcccggac gccggccggg
    14401 ccggtcgccc gcgccaaaac gaggaccacg ggccgtcccg ggcgcgtgac gctcgccgat
    14461 ttgatcgcgc acgccgtccc gcgcgcctgg ttcgagagcg tcgcgatcgt gcaggagttg
    14521 tgcgcggtgg ccctggcccg cggaccggct cacgaccttc gcgtgcccga actgcggcac
    14581 gtggcgatca cgccgaccgg cgaagtggag ctgctcgccg aaggcccggc cgggcactcc
    14641 cccgtccacc gggcgagcct cgtcctgctg gccctgacgc ccgaagccga gctgccggtg
    14701 cagttgaagc tgctggcgct cgaggagctg tcgccgcggc cgaagctggg gaacctgccc
    14761 gatctccaca aggaactgga gttcttcgag cggcccgacc gtcgagcgat catccagggc
    14821 gtctacgagc gctaccgcgc ccagacggcg ccgtcgaagg ggcttgccgc cgtcccgacg
    14881 ccgctgctcg aaccgtcgca gcccaggcgg cgctggcgct ggtggaggag ccgggggctc
    14941 tggatctccg tggccgtcgc ctcgcttgcc attgccgtcg ccctcgccgt ccgggcggcg
    15001 ccaaggcctg acggccagcg gatgcggctg gccgtatccg acctgtcgcg ggcagcctcg
    15061 aacgcgggcc gcttcgtcgc cgcgaaggcg agtgagcagg ttcgggtcct gaagtggagg
    15121 ctcgggctga acaagcggcc gccgaggagg ctgccggaca tccgggcgag cagtcccgcg
    15181 cccgcgcctg ccgaagccgc ggtcgcgacg ccgccccttg gcccagccga ggccgacgcc
    15241 cggcttccgg ccgccgtcca ggctccaggg gaaccggggg ttccagaggg cgcgccggct
    15301 ccagttcctg tagctccgac agccgcagcc ccaccgtccg cgtcggttcc gcatcgggct
    15361 gcagaggcgg gcgccggcga tccgtcgtcg ggcgctgcgc tgcctgcatc ggcggtcgtc
    15421 tacacggcgt cggacccgct cgtcgtgccg ccggtgctgg tgcggcccac tctcccggcg
    15481 ggtccgcctc ccggcatgag gaccgaggag ttgccggagg tggagatcgt cgtgtcggcc
    15541 gcaggcgacg tcagttcggt gaggctcgtc tccgccgggg ccggagcgcg ccaggcgatg
    15601 atgctgagcg cggtgaaagc atggcagttc aggccggcga caaggggcgg ccagcccgtc
    15661 ccgtaccggc tgcggctgcg gctcccggcg aactgagccc cgaatgtccg tgccgcgcag
    15721 tgacccggcg agaggctaat atctcttccc agccacgccg aaagttctgg cagcagcctg
    15781 actttatgag gctcattcgg gcgaaaccac aagatctagt gcggttttcc cttcacgacg
    15841 gtgtttttgc cgggtatcga aattgctagt ggcgtggcag cgtcattagc tagtccggga
    15901 ctgagtatgc tgggacgtcg gaggcactcg aggtacctgc tcgcgcaacc ggtggagggc
    15961 cacctgcgcg tgcgcgagga ggtcacgatc aagaagtgga gcggcagcga cgtcgtggtt
    16021 ctggcatcgg aaccctgcgc ctgcaacgag cgcctgacgc tcgaagtgcc ggggccgctc
    16081 ggccgccgct tgcatgtgca ggttgtcgaa tgccgcccgg cggtcacgga cgagggggcc
    16141 atccttcacc ggtgccgcct tgcgattctg gaggccgcgg ccgactgcgc cggcccggcg
    16201 gcgagcgagc cgtgagcgtc gaacgaagcc gcggcccctc gcaggcgagc gcgattccag
    16261 tggggacgct ggtccgctcc gtgccggttc gtctcgtcga ggtgagttcc ggcgggtgcc
    16321 tgctggagag cgaccggcgc ctcaaggcag gcacctgcgg tctgctggcc gtgccgctgg
    16381 cagggctcgt gcatgcggac gatgtgaggg tggccaggtg ccagcaggtc gctggcgccg
    16441 gcgcggcgtt caacgtgggg gcggaattgc tgagaacgcg gcggttcggg cgcaggagcc
    16501 ttcgggccgc cgtggcgagg attgtcggcg acggacgggg cgttggccac gcccgtcctg
    16561 atgcggagga gccacagccc gtcggtgtcc ggacggacgg cgcgggtggc aggtgcgaga
    16621 gccgggcgcc gccggcccac tcggacaacg gcccttagac cgggccgccg tgcacacccg
    16681 gggcgtgcgc ctcgggagag actactgttc tctagtgaga gaaccgaaaa gacaagaggc
    16741 taccatgaag aacctgattc ggcgtttcat ccgtgacgag gaaggccagg acatcatcga
    16801 gtacgcgctc ctcgcggcgt tcatctcgat catcgccatc gcggcggttc gcgcagtcgg
    16861 cggccgtgtg caggtctact tcggcaacgt gcagtcggcg ctgagctaga gatacgctgt
    16921 tcgagtgaca ggcacccggc ggggtggccc cgccggg
//
LOCUS       DAOSIF010000013        16681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_3637_length_16681_cov_4.734211, whole genome shotgun sequence.
ACCESSION   DAOSIF010000013 DAOSIF010000000
VERSION     DAOSIF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16681
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3637_length_16681_cov_4.734211"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /locus_tag="PLT35_01105"
     CDS             complement(<1..2353)
                     /locus_tag="PLT35_01105"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG27851.1"
                     /translation="MPAAPAPGERRAASPDAVSIPVTGMTCAACAANIERALKRLPGV
                     SGAGVNYATNRATVAFDPAVLGVPAIVEAIRDVGYDVVTGPEAGPGTAWQAIDGAVDA
                     EEQAREREHRALRRRLIFGAALGLPVVVLGMAHLHAPASNWVQFALATPVLFFCGWRF
                     YRGAWKALRHGTADMNTLVAVGTGAAFAYSAAATVAPAAVSTDPRVVAGQAPAPVYFE
                     TAVVIIVLVLVGRLLESRARGRTSEAIRRLIALQPRTARVIRDGVELDVPARDVLRGD
                     IVLVRPGERLPVDGEVIDGASAVDESMLTGESMPVEKAAGSSVFGGTINTTGAFSFRA
                     TRVGAETALQQIVKLVGEAQSRRAPIARAADAISAWFTPAVIAAAAVTFGAWLAFGPP
                     DSRLATAMVRGVAVLIIACPCAMGLATPTAILVGTGKGAEQGILIRGGDILERAASVT
                     TVVLDKTGTITTGKPEVTDCIPAGMPDPASGLPGPDPAAGPAADAWSAGPAAELLALA
                     AAAEANSEHPLAQAVVRAAKAAGLPVGRAEAFDVLPGRGVRAVVGGSPVAAGSEAFLR
                     ECGIDTSALAHERERLAGLGRTIIVVASGAALRGVIALADRPRPEARAAIARLRASGR
                     QIVMLTGDSAATARAIAAEVAPGGEIGRIVAGVLPGHKAEAVKALQEEGRVVAMVGDG
                     INDAPALAQADVGIAMGSGTDVALEAADIALMRGDLNGVADAIDLSSRTLRVIRQNLF
                     WAFFYNVLGIPLAAGAFSPWTGWQLSPMIAAAAMSFSSVSVLAN"
     gene            2449..3174
                     /locus_tag="PLT35_01110"
     CDS             2449..3174
                     /locus_tag="PLT35_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27852.1"
                     /translation="MRISFDMDGVLADMDGALARVAEQEFGVTPKAAVREAAAGDADQ
                     AADEADGNGAAVLASLSPRQQSRLWQRCRGTRNFWETLDECEPGVVRRVQKLAHDLQW
                     DVLFVTQRPPSVGRTTQLQTQRWLRRHGFEYPAVYTTVGSRGAIAAALTLDAHIDDRL
                     QHCVDVATESKAWPILVWRDPGSFDRIRAGASNLGIAVVAAVAEALDRIAAADRAPAA
                     QADASSLLDRLRRAFKEPPGVKR"
     gene            3376..5088
                     /locus_tag="PLT35_01115"
     CDS             3376..5088
                     /locus_tag="PLT35_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG27853.1"
                     /translation="MPTPDAGRPGRAASLARLFEAVHEGVYIGLLGPDASVTIAANPY
                     FRIMLGYPAETPESDLRPFDPDRFTDEGARVAFVDRLARDGAVTDYLLRLRRADEAAI
                     WVEVTAHAEVDEAAGTLRVEALVRDVSDRKKLEDQSRGLYQQLLQAEKMAALGTTISG
                     VAHELNNPLGTILTWSERLAERDLDASARRGVEAILHEAERAARIVRNLLTFARKRHT
                     TRTMVDMNQLVRETLSMRAYEQRVTNISVIDALASGLPQVFADPHQLQQVLLNLVINA
                     EQAMLAANGRGTLVVRTWQESERESIVLEVNDDGPGVPGEAQAKIFDPFFTTKAVGKG
                     TGLGLTVAYAIVEEHGGRMWLASEPGAGASFFVELPCAGGKPLAPPAEGLEAARPAAF
                     RPAWSAAAPAGDDVSGLVSSGGIGRSRPASDAGGGVPAPPAREPSSGEETPQAPTRLD
                     ARAILVVEDETALASAMVEAFSDAGYLVERAGDGEEALARLENGQYDLIVSDLKMPRV
                     DGIQLFAVLRQRHPEMAGRLMFVTGDVIGAEAERFLKESGCPWLAKPFRLSELLRKTR
                     ELLA"
     gene            complement(5352..5948)
                     /locus_tag="PLT35_01120"
     CDS             complement(5352..5948)
                     /locus_tag="PLT35_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27854.1"
                     /translation="MTRTLPTLVATLALTATAGAACSSSSTESAGAPAATPAYSKTTG
                     KLELITYDTNRDGKADAWAHMDGTRLVRMELDRDFDGVIDRWEHYDADGALEKVGFSR
                     AGDGIPDAWAFQDSSGRLARIDVSTRRDGTVNRREYYEGGLQIRAEEDTDEDGKPDKW
                     ETYRDGTLFSVTLDTDRDGRPDRRLTYLPSGVTSEKLR"
     gene            6164..7585
                     /locus_tag="PLT35_01125"
     CDS             6164..7585
                     /locus_tag="PLT35_01125"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG27855.1"
                     /translation="MALRHILFVEDEAPLRTAVAEQLADRGFHVEQAASGEEAVDRLS
                     SFAFDIVITDLRLGAGPDGSAVLDEARALYPDIIGIIVTGYGTVKDAVEAIKRGATDF
                     IQKPFQLEELLHVIDVALEQRRLRTENAYLRKQLEERYRFEGIIGKSAAMKELFALLE
                     TVAQTSSTILITGETGTGKELAAHAIHHNSPRRGQRFVAIHCGAIPETLLEAELFGHV
                     RGAFTGAVATRAGRFEEANRGTLFLDEVGTMTPALQMKLLRVIQGREVQRLGDTGVIK
                     VDVRIIAATNHDLAKMVAEGAFREDLYYRLAVIPVHMPPLRDRKDDVPLLTQHFLAKY
                     GSALARDRDPGLPPPQVGIAQEALRHLMSHAWPGNVRELENAIERAVAFTGGHRPIEV
                     RDLPPAIQAAGTSIAPLDMTIPEQGFDLPGYIDRLEREVIARALERTGGNKGAAAKLL
                     NLKRTTLVEKLKRMGRAGAPRAS"
     gene            complement(7595..9082)
                     /locus_tag="PLT35_01130"
     CDS             complement(7595..9082)
                     /locus_tag="PLT35_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG27856.1"
                     /translation="MPWFVLLLVAWGALAFGAVYPWARWTLLALCVIAGAWGFLRPAP
                     PERRRIHGAVLAALGAVALAVLLQLVPLDRGTLRRWSPATDEFLMQYDLRYAAAVRFA
                     GRFRAAAPGDGTGATADAGSGAAAAPAPAAARAWHALSIDPPKTRVGLAMLVGFAFML
                     LGLARGLGGGDLRVIAPGLAALGVLMAMIGIVQRALWAGKVYGFWEPINKNVTAFGPF
                     INRNHFAGWMLLAIPVAAACVASAAARGMAGVSPGWRNRVLWFSTPAASRAVLAGFAL
                     LAMGLALALTLSRSGIAGFLVAMVLSALHVLRRQRASAKGRVLSTCLVLVFAAAIAWV
                     GIDAVADRFAALDWQLAGRAGAWEDAWRIHRAFPWFGIGLNTYGSATVILQQFERATH
                     HFIEAHNDYLQLLVEGGWLVAVPALALVLVFAREVRKRFREGRDDRTGYWIRLGAVTG
                     IVAIALQETVEFSLQMPGNAALFTVLCAIAMRRATAKRGGQRPAA"
     gene            complement(9109..10116)
                     /locus_tag="PLT35_01135"
     CDS             complement(9109..10116)
                     /locus_tag="PLT35_01135"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOG27857.1"
                     /translation="MTILVTGVAGFIGFHVASRLLDRGDAVIGLDNLNAYYDVTLKEA
                     RLARLRERPAFRFVKLDLADREGMAGLFAAERPPRVVHLAAQAGVRYSLENPHAYIDS
                     NIVGFTNVLEGCRRHGVEHLVYASSSSVYGGNARLPFSEHDNVDHPVSLYAATKKANE
                     LMAHTYSHLFGLPTTGLRFFTVYGPWGRPDMALFLFTKAILAGRPIDVFNHGRMVRDF
                     TYIDDVVEGVVRVLDRAADADPAFDAARPDPGTSHAPYRVFNIGNSQPTPLEDYIEAL
                     EEALGRKAQRAYLPMQPGDVPATSADTRELDAWVGFKPGTPVRDGVRRFVEWYRGYYR
                     V"
     gene            complement(10164..11015)
                     /locus_tag="PLT35_01140"
     CDS             complement(10164..11015)
                     /locus_tag="PLT35_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOG27858.1"
                     /translation="MVCLAAAAALAFAVASAAQAPQTLQAGGGGQGEYIIGPLDVLSI
                     TVWESPDLSGKFSVEADGSFTFPLIGRVKAGGLTLRQFEGELKAKLADGYFKNPQVSV
                     AVETYRSQRIFIVGEVRNPGTYPLTGDMTLIEAIARAGSTTPNASLEAIVVRAAAGRA
                     ADGPTLPGQQHPAAETRRVNLRDLQSGALSENLALRDGDTIFVPRAESIYVFGQVKNP
                     GAYALQTPDTTVLQALSLAGGVTDRGATNRIRIVRVVKGAKQELKVKLGDRVQPGDTI
                     IVPERFF"
     gene            complement(11026..12633)
                     /locus_tag="PLT35_01145"
     CDS             complement(11026..12633)
                     /locus_tag="PLT35_01145"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOG27859.1"
                     /translation="MTRLRPEAECGPRRFQGPPGALLHQALGGTIFFRHLEDLPARVQ
                     ARLASLLREREFVENPRGEARLFPLRPIAAVEPDFQARLDDRRVRLDLQRRFAEFSIA
                     VLPSRRHARARRRRASRNHPAGYTVLVRVRLRASLLLSVLLLAALPEAVLTQSPLYDG
                     WADARWRFGPLAVTPRVELRNLGWDSNVFNEEADPKRDFTATLAAPIDWWLRFGRGRI
                     HGVNTLESVYFGKYVSQRGFNQRHDLTLFFPLNRVQPYAGGTYLSTHDRPDFEINARI
                     RHREAAVTGGAVVRLASRLAVDLGARQTTYRYDEDDDFGAVYSSLFDRRRASYGAQFR
                     YRLTPLTTLTLLADSVHERYRQTRERNHDGFRLLPGVELGRHALVTGRAQVGYRRLNS
                     TSAEMPDFSGLVADAELSYVFGGSLRAALGFSRDILFSYWVDQPFYIQSGFNASLTRQ
                     VSGPWDVQARLARYSLDYQQAARAGLGAAAPRVDRYNSWGGGIGYRVGRDIRVGFNLD
                     SVRRDSVVENLDYRGVRGGMAVTYVLR"
     gene            complement(12846..12983)
                     /locus_tag="PLT35_01150"
     CDS             complement(12846..12983)
                     /locus_tag="PLT35_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27860.1"
                     /translation="MTRSRRRRPAARGEVWVWVWLMDGTMKTTEALVGVVADMGCQIL
                     R"
     gene            complement(13089..>14310)
                     /locus_tag="PLT35_01155"
     CDS             complement(13089..>14310)
                     /locus_tag="PLT35_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOG27861.1"
                     /translation="INAQLEVAGRVSGRVKDLLDHGLPATVTLYSTTDPFTPRASLVA
                     RADTGAFTFNNLKAGTYRLYVNPIGTSLGPQWFMRAAEFGRATPITVTAGVTTSGIDV
                     VLVPPVSAGDFTEDLKSDVLWRHKTNGDMWLWAMDGVSNHVDYYVRTVAEADWEIRAV
                     ADFTGDQRPDLLWRNKSTGQLYLWSMDGAASRAEAYVGTVDLAYDIESYGDYNGDGKA
                     DLLWRAANGELWAWIMDGPVAVDQVYMGIIDPVYRIEASADFNGDGLCDVLWRGTTQG
                     DVWIWLVQDSEEPIEAYVGVVPDAGYQIEAAADFGGDGKADILWRHATHGDVWLWTMD
                     GETKSSEAMIGVVADTNYEIVSTGDYNGDFATDILWWNSANGEVWVWLMDGPYKIEEA
                     LIGIVPDTDYRVVR"
     assembly_gap    14311..14320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14321..15855)
                     /locus_tag="PLT35_01160"
     CDS             complement(<14321..15855)
                     /locus_tag="PLT35_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG27862.1"
                     /translation="MVTSLARVRPLVLASVAALLLLAQATTRAGGPSPNPKAFTADLA
                     IDEMHADPRPFDIAGTDIGRSVFFINNGPDAAGNAYVRLSTPAYTTFVSATTSSEGWI
                     LDSAPAVGGTGDIVFVNPTGPAAGQPRARFDIMLSLDPAVPHNTTITTWAATYTTDPE
                     TTDPVSANNTMSLARTAVTVEDPAEPNETPATAYPLPMGTSSGFLYSVGDTWDRDWYR
                     FTIPPADAGKDLKVNVRVTSPYPSPLPANWSSDLDFSILDSSVTLKAMAMSSNDDETL
                     YLHNVEAGDYYIYIDYSSTDYAEGNRFARYSISIETGTDFGVGYITGRVMLDGGTSGV
                     AHAIVRAPVYPYNTVTWHTSFTSVTTDGDGYFTLATAPGDHFLGITGDGNISVNNPEV
                     NIVDEYYQDSERVQDAQSITVAPGVLSNLGTIYVTLGATISGQVTYIDGAPLHNVAVS
                     AYHSLTNISASSYTYTDTNGNYTLRRVPSPWAKVRFSGGGYGEEWYNDQPAHLPAANL
                     NTTP"
     gene            complement(15960..16367)
                     /locus_tag="PLT35_01165"
     CDS             complement(15960..16367)
                     /locus_tag="PLT35_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOG27863.1"
                     /translation="MKRQYLLRRRGRRSGSRLGRPLHTPSFGPFHSASRAAADFTGDG
                     MADLMWRNSATGEVYCWTMNGSDVVGDGYVGTVDTAYDIEGAGDYDGDGKADLLWRRT
                     SIGEVWVWLMNDALKSAEALVGVVADTQYEIGW"
     gene            complement(16479..>16681)
                     /locus_tag="PLT35_01170"
     CDS             complement(16479..>16681)
                     /locus_tag="PLT35_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27864.1"
                     /translation="EHCVGVVANTDYRIQAAGDYNGDQKADILWRNVVAGDMWIWLMD
                     GPAKASEAYLGTVGDPGYQIVK"
BASE COUNT         2497 a         5893 c         5822 g         2459 t
ORIGIN      
        1 tgttggcgag caccgacacc gacgagaagg acatggccgc cgccgcgatc atcgggctga
       61 gctgccagcc cgtccacggc gagaaggcgc ccgccgccag cgggatgccc agcacgttgt
      121 agaagaacgc ccagaacagg ttctgccgga tgacgcgcag cgtccggctg gacaggtcga
      181 tcgcgtcggc gacgccgttg agatcgcccc gcatcaacgc gatgtcggcg gcctcgaggg
      241 ccacgtccgt gcccgatccc atggcgatgc cgacatcggc ctgcgccagc gcgggggcgt
      301 cgttgatgcc gtcgccgacc atggccacga cgcggccttc ctcctgcagg gccttgacgg
      361 cctccgcttt gtggccgggc aggaccccgg ccacgatcct gccgatttcg ccgcccggcg
      421 ccacctcggc ggcaatcgcc cgcgcggtgg cggcgctgtc gccggtgagc atcacgatct
      481 gccgccccga tgcgcgcagc cgggcgatgg cggcccgcgc ttcgggcctc ggccggtcgg
      541 cgagcgcgat gacgccccgc agcgccgcgc ccgacgccac gacgatgatc gtgcgcccga
      601 gcccggccag ccgctcccgc tcgtgcgcga gcgcggaggt gtcgatcccg cactcgcgaa
      661 ggaaggcctc gctgcccgcg gcaaccgggc tgccgccgac caccgcgcgc acgccgcgcc
      721 ccggcagcac gtcgaacgcc tcggcgcgcc ccacgggcag gccggccgcc ttcgcggcgc
      781 gcacgacggc ctgcgccagc gggtgctccg agttcgcctc cgccgccgcg gccagggcga
      841 ggagttccgc ggcagggccg gcgctccacg catccgccgc cgggcccgcg gcgggatccg
      901 gcccgggcag gccggacgcc gggtccggca tccccgccgg gatgcagtcg gtgacctccg
      961 gcttccccgt ggtgatcgtg cccgtcttgt ccagcacgac ggtggtgacc gacgccgcgc
     1021 gctcgaggat gtcgccgccc cggatgagga tgccctgctc ggcgcctttg cccgtgccga
     1081 ccaggatcgc cgtcggcgtg gcgagcccca tcgcgcacgg acatgcgatg atgagcaccg
     1141 ccaccccgcg caccatcgcc gtcgcgagcc tcgagtcggg agggccgaag gcgagccacg
     1201 cgccgaaggt gacggccgcg gcggcaatca ccgccggcgt gaaccacgca gagatcgcgt
     1261 cggccgcgcg cgcgatcggc gcccggcggc tctgcgcctc gccgaccagc ttcacgatct
     1321 gctgaagggc cgtctcggcc cccactcgcg tcgccctgaa agaaaacgcg ccggtggtgt
     1381 tgatggtccc gccgaacacc gaggagccgg ccgccttctc cacgggcatc gactcaccgg
     1441 tcagcatcga ctcgtccacg gccgacgcgc cgtcgatcac ctcgccgtcc accggcaggc
     1501 gctcgcccgg ccgcaccagc acgatgtcgc cccggagcac gtcgcgggcg ggcacgtcca
     1561 gctcgacgcc gtcgcggatg acgcgggccg tgcgcggctg cagggcgatg agccgccgga
     1621 tcgcctccga cgtccggccg cgggcgcggg actcgaggag ccggccgacg agcaccagga
     1681 cgatgatgac aacggccgtt tcgaagtaga ccggcgcagg cgcctggcca gccacgacgc
     1741 gcggatcggt cgagaccgcc gccggcgcga ccgtggccgc ggcggaatac gcgaacgccg
     1801 ccccggtgcc gacggcgacc agggtgttca tgtcggccgt cccgtggcgc agagccttcc
     1861 acgcgccccg gtagaagcgc cagccgcaga agaagagcac cggcgtcgcc agcgcgaact
     1921 gcacccagtt ggaggccggg gcgtgcaggt gcgccatgcc cagcacgacg acgggcaggc
     1981 cgagcgccgc cccgaagatc aggcgccggc gcagggcgcg gtgctcgcgt tcgcgcgcct
     2041 gctcttcggc gtcgacggcg ccgtcgattg cctgccaggc ggtcccgggt cccgcttccg
     2101 gcccggtgac gacgtcgtag ccgacatcac ggatggcctc gacgatggcg ggcacgccga
     2161 gcaccgcggg atcgaacgcc acggtggcgc ggttcgtggc gtagttcacg ccggcgccgc
     2221 tgacgccggg cagccgcttc agcgcccgct cgatgttcgc cgcgcacgca gcgcaggtca
     2281 tccccgtcac gggaatcgat accgcgtcgg gcgaggccgc tcggcgctcg ccgggggctg
     2341 gagccgcagg catgttccta ttgttgatcg gccgggccgg cctccgcgct cgcgccggcc
     2401 gatccggatt ccggatgccg gcttcccgtc ttctgccata atcctcccgt gcgcatctcc
     2461 ttcgacatgg acggcgtgtt ggcggacatg gacggcgccc tcgccagggt ggcggagcag
     2521 gagttcggcg tcacgccgaa ggccgcggtt cgcgaagctg ccgccggcga cgcggatcag
     2581 gccgccgacg aggccgacgg caacggcgcg gccgtcctgg ccagcctcag ccccaggcag
     2641 cagtcgcgcc tctggcagcg ctgccgcggc acgcgcaatt tctgggagac gctcgacgaa
     2701 tgcgagccgg gcgtcgtgcg ccgcgtgcag aaactcgcgc acgacctcca gtgggacgtc
     2761 ctcttcgtca cccagcgccc gccgtcggtc ggacggacga cgcagctcca gacgcagcgc
     2821 tggctgcggc ggcacggatt cgagtacccg gccgtctaca cgaccgtcgg atccagaggc
     2881 gccatcgccg cggcgctcac gctcgacgcg cacatcgacg accggctcca gcactgcgtc
     2941 gatgtcgcca cggagtcaaa ggcctggccc attctggtct ggcgcgatcc cggatccttc
     3001 gatcgaatcc gcgccggcgc ctccaacctc ggcattgccg tcgtcgccgc cgtcgccgag
     3061 gctctcgatc ggatcgccgc ggccgaccgc gcgcctgccg cccaggccga cgcctcctcc
     3121 ctgctcgacc ggctgcgccg cgccttcaag gagccgcccg gcgtcaagcg gtgaggcgcc
     3181 ggatcgggcc cggccctatg cgtgtcggcc gggaatggga cttgggtgcg ggcgactggg
     3241 aacagggggc tggacgctgg ggatcggggc tctgaattcc ggattctgac tcctgacttc
     3301 tggcttctga cttcttctgt ctcctcttcg ccccgcctgc ccatcccttc tccgccgccc
     3361 ctgtataatc ctcccgtgcc cactcccgac gcaggccgtc ccggacgggc cgcatcgctt
     3421 gcgcggctgt tcgaagccgt tcacgaaggg gtctacatcg ggcttctggg cccggacgcc
     3481 agcgtcacca tcgcggccaa cccgtatttc cggatcatgc tgggctatcc ggccgagacg
     3541 ccggaatccg atctgcggcc gttcgacccg gatcggttca ccgacgaagg cgcccgcgtc
     3601 gccttcgtcg atcgcctcgc gcgggacggc gcggtcacgg actacctgct gcggctccgg
     3661 cgcgccgacg aggccgccat ctgggtcgaa gtgactgcgc acgccgaggt ggacgaggcg
     3721 gccggcacgc tgcgcgtcga ggcgctggtg cgcgacgtga gcgatcgcaa gaagctcgag
     3781 gaccaatcgc gcggcctcta ccagcagctg ctgcaggcgg agaaaatggc ggccctgggc
     3841 acgaccatct cgggcgtggc gcacgagttg aacaaccctc tcggcacgat cctgacgtgg
     3901 tccgagcggc tggcggagcg tgatctggac gcttccgccc ggcgcggcgt ggaggcgatc
     3961 cttcacgagg ccgagcgcgc ggcgcgcatc gtgcgcaacc tgctgacgtt tgcccggaag
     4021 cggcatacca cccgcacgat ggtggacatg aaccagctgg tgcgggaaac gctgtccatg
     4081 cgcgcctacg agcagcgcgt gacgaacatc agcgtgatcg atgcgctcgc atcgggcctg
     4141 ccccaggtct tcgccgaccc gcaccagctc cagcaggtgc tcctgaacct cgtgatcaac
     4201 gccgagcagg ccatgctcgc ggcgaacggg cgcggcacgc tcgtcgtgcg gacgtggcag
     4261 gagtcagagc gcgagtccat cgtgctcgag gtgaacgacg acgggccggg cgtgccgggt
     4321 gaggcgcagg cgaagatctt cgatccgttc ttcacgacga aggcggtggg caaggggacc
     4381 ggcctcggcc tgacggtggc ctacgcgatc gtcgaggagc acggcggccg gatgtggctc
     4441 gcgtccgagc cgggcgccgg cgcgtccttc ttcgtcgaac tgccctgcgc gggcggcaaa
     4501 ccgttggcgc caccggcaga ggggctcgag gcggcccggc ccgccgcgtt ccgccccgcc
     4561 tggagcgcgg cggcgccggc gggcgacgat gtgagcggcc tggtgagctc cggggggatc
     4621 ggccgatcgc ggccggcatc cgacgccggc ggaggcgtac cggcgccgcc tgcgcgtgag
     4681 ccgtcctcag gcgaggaaac gccgcaggcc ccgacgcgcc tcgacgcgcg cgcgatcctc
     4741 gtcgtcgagg atgaaaccgc gctggcttcg gccatggtcg aggcgttcag cgacgcgggg
     4801 tacctcgtgg agcgggcggg cgacggcgag gaggccctgg cacggctgga gaacggccag
     4861 tacgatctca tcgtcagcga cctgaagatg ccgcgcgtgg acggcatcca gttgttcgcc
     4921 gtgctgcgcc agcgccatcc ggagatggcc ggccgcctga tgttcgtgac cggtgatgtc
     4981 atcggcgccg aggccgagcg gttcctgaag gaaagcggtt gcccctggct cgccaagccc
     5041 ttcaggctga gcgaattgct gcggaaaacg cgcgagttgc tggcctagaa ggcgggcggt
     5101 tccccccgaa cccgggccgc tcctgctctg tctgcctctt tcggaggctt cgagattgac
     5161 tatccggcct gggggcccgg agcagacccg cccgtgcgac tggcgagcag cgactgcggc
     5221 ccgttcacgc ggccggtgag cgggctgacg tccgcgtcga cagcttggcg ggcgtgacgg
     5281 gtgccgcagc cgacggccat tcctggcccg acgggggaag gcaaccggga agattggacc
     5341 cggcggacgc gttatctgag cttctccgag gtgacaccag acgggaggta ggtcagacgc
     5401 ctgtcgggac ggccgtcgcg gtcggtgtcg agggtcacgc tgaacaaagt gccatctcga
     5461 taggtctccc acttgtccgg cttgccgtcc tcgtccgtat cctcttctgc ccggatctga
     5521 aggccaccct cgtagtactc gcggcggttg accgtgccgt cgcgccgcgt cgagacgtcg
     5581 atgcgcgcga ggcggcccga ggagtcctgg aacgcccagg catcggggat gccgtcgccg
     5641 gctcgcgaga agcccacttt ctcgagcgcg ccgtcggcgt cgtagtgctc ccaccggtcg
     5701 atcacgccgt cgaagtcgcg gtcgagctcc atgcgcacga ggcgcgtgcc gtccatgtgc
     5761 gcccaggcat cggccttccc gtcgcggttc gtgtcgtagg tgatgagctc gagcttcccg
     5821 gtcgtcttcg agtaggccgg cgtggccgcg ggcgccccgg ccgattccgt ggaggacgag
     5881 ctgcaggcgg cgcctgcggt cgcggtgaga gccagcgttg cgacgagggt gggaagcgtc
     5941 cgggtcacag gcactcagta tatggggtca gatctaaaat tccggcaccg cttcaccgga
     6001 tggggttgga tttggggtca gatctaaaat tccggcaccg ctcacacgca cggtgccgga
     6061 attttagatc tgaccccatt gaccggtgag gcggcatacg tcaaacttct gacgttctct
     6121 gggcggcgtg tggccgctct gggcccgaac cggcagtccc gacatggcat tgcgacacat
     6181 cctcttcgtc gaagacgaag cgccgcttcg cacggcggtg gccgagcagc tcgccgatcg
     6241 cggcttccac gtcgaacagg ccgcgagcgg ggaggaggcg gtcgaccgcc tgtcctcctt
     6301 cgccttcgac atcgtgatca ccgacctgcg cctgggcgcg ggcccggacg ggtcggcggt
     6361 gctcgacgag gcccgggcgc tctaccccga catcatcggc atcatcgtca cgggctacgg
     6421 caccgtgaag gacgccgtgg aagccatcaa gcgcggcgcg acggacttca tccagaagcc
     6481 gttccaactg gaggagctgc tgcacgtcat cgacgtcgcg ctcgagcagc gccggctccg
     6541 gaccgagaac gcgtacttgc gcaagcagct cgaggagcgc taccgcttcg agggcatcat
     6601 cggcaagagc gcggcgatga aggagctctt cgcgcttctc gagacggtgg cgcagacgag
     6661 cagcacgatt ctcatcaccg gcgagaccgg gacgggcaag gagctggccg cccacgccat
     6721 ccaccacaac agcccgcggc gcggccagcg gttcgtcgcc atccactgcg gcgcgattcc
     6781 cgagacgctg ctggaggccg agctcttcgg ccacgtccgc ggcgccttca ccggcgccgt
     6841 cgccacccgg gcgggccgtt tcgaggaggc gaaccggggc acgctgttcc tggacgaggt
     6901 gggcacgatg acgccggcgc tgcagatgaa gctcctgcgc gtcatccagg gccgcgaggt
     6961 gcagcgcctc ggcgacaccg gcgtcatcaa ggtggacgtg cgcatcatcg ccgcgacgaa
     7021 ccacgacctg gcgaagatgg tggcggaagg cgcgttccga gaggacctct actaccgcct
     7081 cgccgtcatc cccgtccaca tgccgccgct ccgcgaccgg aaggacgacg tgccgctgct
     7141 cacgcagcac ttcctcgcca agtacggctc ggccctggcg cgcgaccggg accctggcct
     7201 gccgccgccg caggtcggca tcgcgcagga ggcgctccgg cacctcatgt cgcacgcgtg
     7261 gccgggcaac gtgcgcgagc tggagaacgc gatcgagcgg gcggtggcgt tcacgggagg
     7321 acaccgcccc atcgaggtgc gcgatctgcc gccggcgatc caggccgccg ggacgtcgat
     7381 cgcgccgctc gacatgacga tcccggagca gggcttcgac ctgccgggtt acatcgacag
     7441 gctcgaacgc gaggtcatcg cgcgcgcgct cgagaggacc ggcggcaaca agggcgcggc
     7501 ggcgaagctg ctcaacctga agcggacgac gctggtggag aagctgaagc ggatgggccg
     7561 cgccggggcc ccgcgcgcct cgtagccgcg gaggtcacgc cgcgggccgc tggcctccgc
     7621 gcttcgctgt cgcccgccgc atcgcgatcg cgcacagcac ggtgaacagc gccgcgttgc
     7681 ccggcatctg caggctgaac tccacggtct cctggagcgc gatcgccacg atcccggtca
     7741 ccgcgccgag gcggatccag tagcccgtcc ggtcgtcgcg cccctcgcgg aaccgcttcc
     7801 gcacctcgcg ggcgaacacc agcacgagcg ccagcgcggg aacggccacc agccagccgc
     7861 cctccaccag cagctgcagg tagtcgttgt gcgcctcgat gaagtggtgg gtggcgcgct
     7921 cgaactgctg caggatcacc gtcgccgacc cgtacgtgtt gaggccgatg ccgaaccagg
     7981 ggaacgcccg gtggatgcgc caggcgtcct cccaggcgcc ggcgcggccg gccagctgcc
     8041 agtccagcgc ggcgaaccgg tcggccacgg catcgatgcc cacccacgcg atggcggccg
     8101 cgaacaccag gaccaggcac gtcgacagga cccggccctt cgccgacgcg cgctggcggc
     8161 gcagcacgtg cagggccgag agcaccatcg cgacgagaaa gccggcgatg cccgagcggg
     8221 acagcgtgag ggcgagcgcc agtcccatcg cgaggagcgc gaagccggcg aggacggccc
     8281 ggctggccgc cggcgtggag aaccagagca cgcggttccg ccaccccgga ctcacgccgg
     8341 ccatgcccct ggccgccgcc gacgcgacgc aggccgcggc cacggggatc gccagcagca
     8401 tccagcccgc gaagtggttc cggttgatga agggcccgaa ggccgtcacg ttcttgttga
     8461 tcggctccca gaagccgtac accttcccgg cccagagcgc ccgctggacg atgccgatca
     8521 tcgccatgag gacgccgagg gccgccaggc ccggcgcgat cacgcgcaga tcgcctccgc
     8581 cgaggccgcg ggccagcccc agcagcatga acgcgaagcc gacgagcatc gcgagcccga
     8641 cgcgcgtttt cggcggatcg atggagagcg cgtgccacgc gcgagcggcg gccggcgcgg
     8701 gcgcggcggc agcgccgctg ccggcatcgg ccgtcgcgcc agtcccgtct cctggcgccg
     8761 ccgcgcggaa ccggccggcg aacctcaccg ccgccgcgta ccgcaggtcg tactgcatca
     8821 ggaactcgtc ggtggcgggg ctccagcgcc gcagcgtccc ccggtccagc ggcacgagct
     8881 gcaacaagac ggcaagagcg acggcgccaa gggcggcgag aacggcgccg tggatgcgcc
     8941 gccgctcggg aggcgcgggc cgcaggaacc cccacgcccc ggcgatgacg cacagcgcca
     9001 ggagagtcca cctcgcccag ggatagacgg ccccgaacgc cagcgcgccc cacgccacga
     9061 ggagcagcac gaaccagggc atgatcgatc cgtcgggtct gccggcgcct agacccggta
     9121 gtacccgcgg taccactcga cgaagcgccg cacgccgtcc ctgacgggcg tgcccggctt
     9181 gaatcccacc cacgcgtcca gctcccgcgt gtcggccgat gtcgccggca cgtcgccggg
     9241 ctgcatcggc aggtaggccc gctgcgcctt ccgcccgagg gcctcctcga gcgcttcgat
     9301 gtagtcctcg agcggcgtgg gctggctgtt cccgatgttg aacacccggt acggcgcgtg
     9361 gctcgtgccg gggtccggcc gggcggcgtc gaacgccggg tcggcgtcgg ctgcgcgatc
     9421 cagcacccgc acgactccct cgacgacgtc gtcgatgtag gtgaagtcgc ggaccatgcg
     9481 gccgtggttg aagacgtcga tcgggcggcc cgcgaggatg gccttcgtga agaggaacag
     9541 cgccatgtcg gggcggcccc agggcccgta caccgtgaag aagcgcagcc ccgtcgtcgg
     9601 aaggccgaac aggtggctgt aggtgtgggc catcagttcg ttcgccttct tcgtggccgc
     9661 gtacaggctg accgggtggt cgacgttgtc gtgctcggag aacgggaggc gcgcgttgcc
     9721 gccgtagacg ctcgacgaac tcgcgtagac caggtgttcg acgccgtgcc ggcggcaccc
     9781 ctcgaggacg ttcgtgaagc cgacgatgtt gctgtcgatg taggcgtggg ggttctcgag
     9841 ggagtagcgg acgccggcct gcgccgccag gtgcaccacc ctgggcgggc gctccgcggc
     9901 gaacaggccg gccatgccct cgcggtcggc gagatcgagc ttcacgaacc ggaacgccgg
     9961 ccgctcccgg agccgcgcga ggcgcgcctc cttgagcgtc acgtcgtagt acgcgttcag
    10021 gttgtcgagg ccgatcacgg cgtcgccgcg gtcgagcagc cggctggcga cgtggaagcc
    10081 gatgaacccc gccacgcccg tcacgagaat cgtcatgggc ctgcttccag tgggcgcgcc
    10141 cgcgaagccg gtcgcgccgc gaatcagaag aaccgttccg gcacgatgat cgtgtcgccc
    10201 ggctgcacgc ggtcgccgag cttgaccttc agctcctgct tggccccctt gacgacgcgc
    10261 acgatcctga tgcggttcgt cgcgccgcga tccgtgacgc cgccggcgag cgacagcgcc
    10321 tgcagcaccg tcgtgtccgg ggtctggagc gcgtaggcgc ccgggttctt gacctggccg
    10381 aagacgtaga tcgattcggc ccggggcacg aagatcgtat cgccgtcgcg gagcgcgagg
    10441 ttctccgaca gcgcgccgct ctgcaggtcg cgcaggttca cgcgccgcgt ttccgccgcg
    10501 gggtgctgct ggcccggcag cgtcggccca tcggcggccc ggcccgcggc cgcgcggacc
    10561 acgatcgcct cgaggctggc attgggcgtg gtcgagccgg cccgcgcgat cgcctcgatg
    10621 agcgtcatgt cccccgtcag cgggtaggtc ccgggattgc gcacttcgcc gacgatgaag
    10681 atccgctggc tgcggtaggt ctcgacggcg acgctcacct gcgggttctt gaagtagccg
    10741 tcggcgagct tcgccttgag ctccccctcg aactgccgca gcgtcagccc tccggccttg
    10801 acccggccga tgagcggaaa ggtgaagctc ccatcggctt ccacgctgaa cttgccggag
    10861 aggtcggggg attcccacac ggtgatcgag aggacgtcga gcgggccgat gatgtactcg
    10921 ccctgccccc cgcccccggc ctgcagcgtc tggggcgctt gcgccgccga cgcgaccgcg
    10981 aaggccagcg ccgcggcggc cgccagacag accacgcgtc gcacgctacc tgagcacata
    11041 ggtaaccgcc atgcccccgc ggaccccgcg gtagtcgagg ttctcgacga ccgagtcgcg
    11101 gcggacggag tcgaggttga accccacgcg aatgtcgcgg ccgacacggt acccgatgcc
    11161 gccgccccag ctattgtagc ggtccacccg cggggcggca gccccgagtc cggcccgcgc
    11221 ggcctgctgg tagtcgaggc tgtaccgtgc gagcctcgcc tggacgtccc acgggcccga
    11281 cacctgccgg gtgaggctgg cgttgaagcc ggactggatg tagaagggct gatcgaccca
    11341 gtacgagaag aggatgtccc gcgagaaccc gagcgcggcc cgcagactcc cgccgaacac
    11401 gtacgacagc tcggcgtcgg ccaccaggcc cgagaagtcc ggcatctccg cgctcgtcga
    11461 gttcagcctc cggtacccga cctgagcgcg gcccgtcacg agcgcgtgcc ggccgagctc
    11521 gacgccgggc agcaggcgga agccgtcgtg gttccgttcg cgggtctgcc tgtagcgctc
    11581 gtgcacgctg tcggcgagga gcgtcagggt ggtcaggggc gtcaggcggt agcggaactg
    11641 cgcgccgtag ctcgcgcggc ggcgatcgaa cagcgaggag tagacggcgc cgaaatcgtc
    11701 gtcttcgtcg taccggtagg tcgtctggcg cgcccccaga tccacggcca gccggctcgc
    11761 gaggcggacg accgccccgc cggtgaccgc cgcttcgcgg tggcggatgc gcgcgttgat
    11821 ttcgaaatcg ggccggtcgt gcgtgctcag gtacgtgccc ccggcgtagg gctggacgcg
    11881 attgagcggg aagaagagcg tgagatcgtg ccgctgattg aacccccgct ggctgacgta
    11941 cttgccgaag tagacgctct cgagcgtgtt tacgccgtgg atccgcccgc gcccgaaccg
    12001 cagccaccag tcgatcgggg ccgccagcgt cgccgtgaag tcccgcttgg gatccgcctc
    12061 ctcgttgaac acgttcgagt cccagcccag attccgcagc tcgaccctcg gcgtcacggc
    12121 cagcgggccg aaccgccacc tggcgtccgc ccagccgtcg tagagcggtg actgggtcag
    12181 gaccgcttcg ggaagcgccg cgaggaggag aacggacagc aggagggagg cccggagacg
    12241 cacgcgcacg aggacagtgt aacccgccgg atggttccgc gaggcccgcc tgcgacgggc
    12301 gcgggcgtgc cggcgggacg gcagcaccgc gatgctgaac tccgcgaaac gccgctgcag
    12361 atcaagccgc acccgcctgt cgtccaggcg ggcctggaag tcgggctcga cggcggcaat
    12421 gggcctgagc gggaacagcc gcgcctcgcc tcgcgggttc tccacgaact cgcgctcgcg
    12481 caggagggat gcgagacggg cctgcacccg cgcgggaagg tcctccaggt gccggaagaa
    12541 gatcgtgccg ccgagcgcct ggtggagcag cgcgcccggt gggccttgga accgacgtgg
    12601 cccgcactct gcttctggac ggagtcgtgt caagcacgag acagggtgtg acgatatcga
    12661 tacaggcggt ggtgccgctc tgcgtgcctc atccctcccg ccgcacgcgg gtggccgggg
    12721 cgctttgcat gtgcccgcag gctggtcacc gtcagttctg ccgaggacac atggagacgt
    12781 cctggacagg acccgctacc gggcgggctg ccaggggcga gcgcgctgcg cctggcccct
    12841 ggcgcctacc tcaggatttg gcagcccatg tccgccacga cgccgacaag cgcttccgtc
    12901 gtcttcatgg tgccgtccat gagccagacc cacacccaca cctcgccgcg cgcggcgggc
    12961 cgcctgcgcc gggagcgggt cacggccagg gacccggcgc tagcggctgg tcggatgcgg
    13021 ctcgcgccgc gtaggatgcc gcagggcgtg acgcgtggac tgacgagccc cgagccccga
    13081 gtccgtggct atcgcacgac ccgatagtcc gtatccggca cgatgccgat gagcgcctcc
    13141 tcgatcttgt acgggccgtc catcagccac acccacactt cgccgttcgc cgagttccac
    13201 cagaggatgt ccgtcgcgaa gtcgccgttg tagtcgccgg tcgacacgat ctcgtagttc
    13261 gtgtcggcga cgacgccgat catcgcctca gacgacttcg tctccccgtc catcgtccag
    13321 agccacacgt cgccgtgcgt ggcgtggcgc cacaggatat cggccttgcc gtcgccgccg
    13381 aagtccgccg ccgcttcgat ctggtagccg gcatcgggca cgacgccgac gtacgcctcg
    13441 atcggttcct cgctgtcctg aaccagccag atccagacgt cgccctgcgt ggtgccgcgc
    13501 cagagcacat cgcacaaacc gtcgccgttg aagtcggcgg acgcttcgat ccggtagacc
    13561 ggatcgatga tccccatgta cacctggtcg acggcaaccg gcccgtccat gatccaggcc
    13621 cacagttcgc cgttggccgc gcgccacaac aggtcggcct tgccgtcgcc gttgtagtct
    13681 ccgtagctct cgatgtcgta cgcgaggtcg acggtgccga cgtacgcttc agcccgcgat
    13741 gccgctccat ccatcgacca gaggtagagc tggccggtcg acttgttgcg ccacaggagg
    13801 tcggggcgct ggtcgcccgt gaagtcggcc acggcccgga tctcccagtc ggcctccgcc
    13861 accgtgcgca cgtagtagtc gacgtggttg gagacgccat ccatggccca cagccacatg
    13921 tccccgttcg tcttgtggcg ccagaggacg tccgacttca agtcctcggt gaaatcgcca
    13981 gcgctcaccg gcggaaccag gacaacatcg atgccagacg ttgtcactcc cgctgtcacc
    14041 gtgatgggag tggccctccc gaattctgcc gccctcatga accattgcgg ccccaggctc
    14101 gtcccgatcg ggttgacgta gagtctgtac gtcccggcct tgagattgtt gaaggtgaac
    14161 gcgcccgtgt cggccctcgc gacgagcgat gccctgggcg tgaaagggtc ggttgtcgaa
    14221 tagagcgtca cggtggccgg aaggccgtgg tcaagcaggt ctttgaccct ccccgacacc
    14281 cgccccgcca cttccagttg cgcgttgatg nnnnnnnnnn ggcgtcgtat tcaggttcgc
    14341 cgccggcaag tgcgcgggct ggtcgttgta ccactcctcc ccgtagccgc ccccgctgaa
    14401 ccggaccttc gcccaggggc tgggaacccg ccgcagcgtg tagttgccgt tcgtgtcggt
    14461 gtacgtgtag cttgacgcac tgatgttcgt cagcgagtgg taagcgctca cggcgacgtt
    14521 gtgcagcggc gccccgtcta tgtaggtcac ttggccgctg atcgtcgcgc ccaaggtcac
    14581 gtagatggta ccgaggttac tcagaacgcc gggagccacg gtgatggact gggcatcctg
    14641 taccctctcg ctgtcctggt agtactcgtc gacgatgttg acctcggggt tgttcacgct
    14701 gatgttgccg tcgcccgtaa tgccaaggaa atgatcgcct ggcgccgtgg cgagggtgaa
    14761 gtagccgtcc ccgtcggtgg tcaccgacgt gaagctggtg tgccaggtga ccgtgttgta
    14821 cgggtagacc ggcgccctga cgatggcgtg cgcgacgccg ctggtccctc cgtcgagcat
    14881 cacgcggccg gtgatgtagc cgacgccgaa gtcggtgccg gtttcaatcg aaatcgagta
    14941 ccgcgcgaac cggttgccct cggcatagtc cgtcgagctg tagtcgatgt agatgtagta
    15001 gtcaccggcc tccacgttgt ggaggtagag cgtctcgtcg tcgttcgagc tcatcgccat
    15061 cgccttcagc gtgaccgagc tgtcgaggat cgagaaatcc aggtcgctgg accagttcgc
    15121 cgggagcgga gaagggtacg gggacgtgac ccgcacgttc actttcaggt ccttccccgc
    15181 gtcagccggc ggaatcgtga atcggtacca gtcgcggtcc cacgtgtcgc cgacggagta
    15241 gaggaagccc gacgaggtcc ccatcggcag cgggtacgcc gtcgccgggg tctcgttcgg
    15301 ctcggccgga tcttccacgg tgaccgcggt cctggcgagc gacatcgtgt tgttggccga
    15361 gacgggatcg gtcgtctccg gatcggtcgt gtaagtggcc gcccaggttg tgatcgtggt
    15421 gttgtgaggg accgccgggt ccaggctcag catgatgtcg aaccgcgccc ggggctggcc
    15481 ggccgccggg cccgttgggt tgacgaagac gatgtcaccc gtgcctccga cggcgggggc
    15541 tgagtccagg atccaccctt cacttgacgt cgtcgcggag acgaacgtcg tgtatgccgg
    15601 cgtgctcagc ctcacgtacg cgttccccgc ggcgtcgggg ccgttgttga tgaagaagac
    15661 cgagcggccg atgtcggtcc cggcgatgtc gaacggcctg ggatcggcgt gcatctcgtc
    15721 gatggccaga tcggcggtga acgccttcgg gttgggcgag gggccgcccg cccgcgtggt
    15781 ggcctgcgcc aggaggagca gggcggcaac cgacgcgagt accagcggcc ggacgcgggc
    15841 gagcgaagta accatggcag tcctcctagc atgcgcagtg aaacgggcag agtatcgcgc
    15901 cgatggccgg tcccgtcaac acgcgggcgc gcgcacgccg agcgcctgat ccccggcccc
    15961 taccacccaa tctcgtactg cgtgtccgcc acgacgccga caagcgcttc cgcgctcttc
    16021 aacgcgtcgt tcatcagcca cacccacacc tcgccgatcg acgtgcggcg ccagagaaga
    16081 tccgccttgc cgtcgccgtc gtagtctccg gcgccctcga tatcgtaggc ggtgtccacg
    16141 gtgccgacgt agccgtcccc gacgacgtcg ctgccgttca tcgtccagca gtagacttcg
    16201 ccggtcgcgg agttgcgcca catcaggtcg gccatgccgt cgccggtgaa gtcggcggcg
    16261 gcgcgggaag ctgaatggaa tggaccgaag gagggagtgt gcaggggacg gcccaggcgg
    16321 ctgccggacc gtcggccgcg acgccgaagc aggtactgac gcttcatggg ggtggccggg
    16381 cgctggcccg ggcgccccgc cacgcgcgta tgctacacct ccttatgtcc accagcaagg
    16441 gctcttcctc aagcccttcg cttcttgtcg gccgttctct acttcacgat ctgatacccc
    16501 ggatccccca ccgtcccgag atacgcctcg gacgccttcg ccggtccgtc catcagccag
    16561 atccacatgt caccggcgac gacgttgcgc cagaggatgt ccgccttctg gtccccgttg
    16621 tagtcccccg ccgcctgaat ccggtagtcc gtgttcgcca ccacgccaac gcagtgttca
    16681 g
//
LOCUS       DAOSIF010000014        15398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_3992_length_15398_cov_4.195203, whole genome shotgun sequence.
ACCESSION   DAOSIF010000014 DAOSIF010000000
VERSION     DAOSIF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3992_length_15398_cov_4.195203"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1302)
                     /locus_tag="PLT35_01175"
     CDS             complement(226..1302)
                     /locus_tag="PLT35_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOG27865.1"
                     /translation="MSDANRRDFLRTTSAAMAAAVAVAPLAASPRFRSDEEKRARLAS
                     NCYACRGVFNRDAFNPAQPPSPEARQLQKKYGSITLLDFPQFTKDNFPGIRSIDLLAG
                     LFGDIADPAMFRKHTSWWDGKPTDTLAFDPDAPSSRTWLDKLAGRFAQTGVSGYHISN
                     DIPQHMADLDDDRRKAGIATAKAWMNAASRIGVKAMRVNTAGPFIGPCAELVNGLPRN
                     DLMEKYLEKAILSYRELAEYGDKVDVIVTIENHWGITANPINTRIIISEVNHPRLEAC
                     PDFCNWEHKYMLYHALDDVMPYARTIVHAKYWDRWEDVDIARCVRILNKHNFQGRISL
                     EYETGPWDGIEGTRRLADAVVKAL"
     gene            complement(1455..2144)
                     /locus_tag="PLT35_01180"
     CDS             complement(1455..2144)
                     /locus_tag="PLT35_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27866.1"
                     /translation="MKQALTVRSVLLGVAAVALTVTVGGCQRTSAGDSPAAPTSSGGP
                     FSGGGAALVIRPLVEVTADLLDVTTGYTGTDNWAGQSVTIPGSGSYNNLRFNWYHYNP
                     AGTPVAFGTLYILTQEYTGLVSNLGPATPGYFAESEAVKDGQYIFPAAVTVTGGTCYW
                     FYSDTRGRLTTGFNKDTYTGGDLYVTGYPTEPFRKAQASWLLLPSGEYLKPALGTGVD
                     ANFRLQGAAVQ"
     gene            2313..5261
                     /locus_tag="PLT35_01185"
     CDS             2313..5261
                     /locus_tag="PLT35_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOG27867.1"
                     /translation="MTSRPSLTAPSEPARGGFRSRLASWADDDALRRHEEKAFFVLTL
                     VIGAIVGLVIVAFILLTENLGARLFAPDAASWHRLVIPVLGALVSGVLLARVFQGARG
                     SGIPQTKVALYLEDGLIHLRTVLGKFACSSLSLASGIALGREGPSVHIGAGIASVIGR
                     RLGLSPSHTKRLVPVGAAAAVAAAFNTPVAAVLFTLEEVVEDLHAPVVGSIVLSSATA
                     WMVLRLLLGDEPLFHVPAYELVHPIEFVVYAALGVAGGIVSVAFVKLLLAQRRWFCGL
                     PASTVWFQPVIGGIVTGLIGWAVPAVLGVGYAHVGEALNGKMALGVMALLLGLKLVAT
                     AAGYASGNAGGIFGPSLFLGAMMGGSIGGLVHTWFPDYTGGVGAYALVGMGAAFAGII
                     RAPFTSVVMIFEITRNYAVIVPLMIANLVSYFISSRLQREPIYEALLHQDGVRLPRRR
                     AEREDQLAVGLAMRPARAFVPAGESAAAAAARLGVPAGATETAPAGAGEAGIADPWRR
                     GWPIMDGDSFVGWLGAADLRRALRGGLHDAPARGLAAAVSPPDPSAPSRSLHPDDTLD
                     LALRRMGEAGLDELPVVSRTRPDELLGTVSVTDVVGAYRKGGGDPQPEATPGRNSPRT
                     LLIRLAVVVLIVLIGSGILGHRLRTARLEGAARAFETGRALAAQGREPEAIEQFRRAL
                     AVSGSADVRLELARTLLAVDRPAEAALYFGEMLRADPASGEANLGLARAAGKQGRTGE
                     AILAYSRALVGNWTGRPEQRRIEAGFEFADYLARSGMRDQAVAQLLDLQQAVSGSPGL
                     LLDVGRRFLSLGADAQSASLFRAVITLDPARIDAHLGLGEAELAQDNFEAARDAFRRA
                     EQIDPGNATAASRRRLLTEVLSLNPEQRRLSSGERHRRSLALLSAALAQFDACAKASG
                     AAGALAPAGPLAEAARRTLARRARPASLADAAEENVDVAQQLWSARERQCPASGEPTA
                     VGVLMKHLAR"
     gene            5342..5701
                     /locus_tag="PLT35_01190"
     CDS             5342..5701
                     /locus_tag="PLT35_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG27868.1"
                     /translation="MNVVNLAEKFALFSDHWRPRIVGELNGQQVKLARLKGEFVWHHH
                     DDADELFYVVRGTLRMMLPDGEARVAAGEFVIVPRGVDHLPVADDEVLVLLFEPAATR
                     NTGNVENDRTATDLERI"
     gene            complement(5916..6305)
                     /locus_tag="PLT35_01195"
     CDS             complement(5916..6305)
                     /locus_tag="PLT35_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOG27869.1"
                     /translation="MRRVIVDTSALAGVVFHEAGGADSASRLEGAAVYAPTLLRYELQ
                     QVAAKKCREQPEQAAEVLQALERTLDLRSGIVWLDPHPTDVVLVANATGLSAYDASYL
                     CLAGMLEAELVTQDQRLSAALDPFSGT"
     gene            complement(6302..6535)
                     /locus_tag="PLT35_01200"
     CDS             complement(6302..6535)
                     /locus_tag="PLT35_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27870.1"
                     /translation="MPVNLSVKDVPDHLANALRERARRNHRSLQGEMLAMIEAHVGAR
                     PFPAFELLAAARSAGLKGPDRSTRWVREDRDSR"
     gene            7566..>7812
                     /locus_tag="PLT35_01205"
     CDS             7566..>7812
                     /locus_tag="PLT35_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27871.1"
                     /translation="MKTPCVLAGLLVVALGTVTLEGAQAVVAAEKSIEIPKGTKAEKI
                     GPGHVRFTLPNLRVIDVTGYDARTGAIGLVQVIDPDPP"
     assembly_gap    7813..7822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7823..8034
                     /locus_tag="PLT35_01210"
     CDS             <7823..8034
                     /locus_tag="PLT35_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27872.1"
                     /translation="PTDARTAAKIIDPQPPGKVEITASQARLVKLRVLTRAAARKLPA
                     ADDVTIDDEITWLPARITDKDGVRR"
     gene            8071..8697
                     /locus_tag="PLT35_01215"
     CDS             8071..8697
                     /locus_tag="PLT35_01215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HOG27873.1"
                     /translation="MKRARARRLAPAFAVAVTVTLAHGQSSYEQHLQIRTSGVPHAGN
                     GVFTTVAIPKGAYLGAYTGEFVTDDEYLRRYQANRWQYMMGLLDCAKPHTGGLTTIDG
                     IRGNVVTRMNYAPAEFQNVRFQKICEPPFVGIVALRDIAAGEELWVDYGPNYPYDFMK
                     DEAFVKFFADLRATAPTHDHRGRTGSQISMTCLCSGWAAGSLSMSSKQ"
     gene            complement(8607..9602)
                     /locus_tag="PLT35_01220"
     CDS             complement(8607..9602)
                     /locus_tag="PLT35_01220"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOG27874.1"
                     /translation="MADISAAASQRAEYLARVHRVVDYIEHHLGDPLSLHALAGVANF
                     SPYHFHRVFTACTGETLHRFILRLRLERAASKAARQPHLSLTAIALDCGFGSSAAFSR
                     AFRAAYGRSATEWRREVRKKREALRKDGEERPGSVPYTGSCAPAALAGLAEEGARIMS
                     YDTTPKKAAASVRIETLKPTTIAYVRHTGPYAGNEALFGRLFERLCSWAGPRGLLGPG
                     ARFLTVYHDNPEVTDEDKLRISVAVTVPPGTAAGDGPGGAGVMELEGGLYAVASYELD
                     PSEYGAAWNWVMGEWLPSSGYQPDDRHCFELMLNDPAAHPEHKHVIEIWEPVRPL"
     gene            9739..11007
                     /locus_tag="PLT35_01225"
     CDS             9739..11007
                     /locus_tag="PLT35_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG27875.1"
                     /translation="MTESNHTRREFLKTVAAAGAVSLAAPRGLDAATRRRSIGANDRI
                     RIGIIGCGDRGIGTEMASVHEHAQAENLEVIAVCDPWRVAREAAAAKAKEWFGTDARQ
                     CRTPDELLSLKEVDAVFISSPDHWHAAHLEAAAKAGKHVYVEKPMANEIGELNRAYAA
                     AKASGVVIQVGTQLRSSATAMGCRELLKSGRLGRITRIEQVRNSDKPYWYQYLKPDVR
                     AADLDWQAFTKGRTKKAFDPILYSGWYGYWEFSQGPVPQWGVHFLDLVHFIAGLGIPD
                     TCVCLGGVYTWKDEHRFTAPDQVQALWQYPEGLMISYSTNFGNSAGNLTRFSGDKGTL
                     RLDWSNAVYSAEGGINRDGSIRGENKVEPIEQTDHWVNWYRCMRDGATPNAPLDAGYQ
                     HAVASIMATMSYETGRRARFDRARRTIATD"
     gene            complement(11026..12162)
                     /gene="bshA"
                     /locus_tag="PLT35_01230"
     CDS             complement(11026..12162)
                     /gene="bshA"
                     /locus_tag="PLT35_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HOG27876.1"
                     /translation="MRVGIVCYASLGGSGIVATELARHLARRGHEAHLLSSDIPFRLR
                     ADPGGVVLHTVETPDYPLFHEPQYVISLATRIVQTARERQLDVIHAHYAVPHATAAYL
                     AREILRTECGGRIPAVITTLHGTDITLIGSNPAFADTVRFSIDRSDAVTAVSESLRRD
                     TIERLGTAAPVRVIPNFLDCERFRRVFDAGLRGRLCPQADYDALVVHLSNFRPVKRVD
                     AVVGVFRRIRERIRARLVLAGDGPDGVKVLDEIETHGLGRDVEILPPQEDVVPLLSVA
                     DLFLLPSLQESFGLAALEAMACKVPVVASSVGGLPEVLTHGVTGMLHPPDDLDGMAAS
                     SIRLLSDRAAHAAMAREGRRAVASRYCADAIVPQYEALYRELGA"
     gene            complement(12159..12893)
                     /gene="bshB1"
                     /locus_tag="PLT35_01235"
     CDS             complement(12159..12893)
                     /gene="bshB1"
                     /locus_tag="PLT35_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HOG27877.1"
                     /translation="MTAPSPVDLLAFGPHPDDIEIGMAATIAKHASLGWRVGLCDLTR
                     GELGSNGTPEERVAEGEAAAALLGAAWRLNLGLPDGALADTEPHVGAIVDLVRSARPA
                     AIAVPYADDRHPDHRRAAALLESAIFKAGLRRYPASGAPWRARWACAYFINDSAPPSF
                     VVDVSDVYDLKRRALACHASQFRPEAPDAASTRLTSPLFLQLVESRDAQFGAAAGVRY
                     AEGFSVREPVVLPLLTDRWPGARRRR"
     gene            13089..13625
                     /locus_tag="PLT35_01240"
     CDS             13089..13625
                     /locus_tag="PLT35_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOG27878.1"
                     /translation="MRSSVRRLLPPVVAVLAVAFAHGQTSYERHLEIRPSRIPHAGNG
                     VFTTAAIPKGARLGAYTGEFVTDEEYSRRYQANRWQYMMGLRDCAKPYTGGITTIDGI
                     AGNVFTRMNYAPAEFQNVKFEKTCEPPFVTIVAIRDIAPGEELWVDYGPNYRYDFMKD
                     EAVIEFFARLRAARARRP"
     gene            13714..14718
                     /locus_tag="PLT35_01245"
     CDS             13714..14718
                     /locus_tag="PLT35_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG27879.1"
                     /translation="MTIAFFELEGWERAALAERLSEHDLLFYEDVLSPAHLPALAACA
                     VVSPFIYSRIGPEALAAMPALKMVATRSTGFDHIDVAACRARGIAVSNVPEYGSQTVA
                     EHTFALILALSRKLVPAYRAMRNTEPRSDEARRLRGFDLRGKTLGVVGAGNIGLHVVR
                     MAESFGMRVLVFDTRRRQTLADLLEFEYVPLEALLRESDIVSLHCPATNETRHMINAD
                     SLAIMKRGALLINTARGELVDTAALLAALQSGQLGGAGLDVFEGEAIVREEAQILSRA
                     FDREQLESAIRAHQLLKRDDVIVTPHNGFNSREAVERILDTTVRNIQGFLAGVTQNAV
                     "
     gene            complement(15053..15127)
                     /locus_tag="PLT35_01250"
     tRNA            complement(15053..15127)
                     /locus_tag="PLT35_01250"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15092..15094),aa:Glu,seq:ttc)
BASE COUNT         2259 a         5394 c         5473 g         2262 t
ORIGIN      
        1 ccacgccgcc ggtttcgcgc cgggcggcgt caccctggcc ctcatcacgg caggcctggg
       61 gcatctggcg tgcccggacc ggaccttccc gcccggggcg cagtgaggct tcgggtgcgc
      121 ctctgcgaat cgcccgccag ggccggccga ggcaacggcg ccgatgcgga acgaatcacc
      181 cgcggcacca tcccgcgcct gcgcctgccg cgccgcctgc tgcgatcaga gcgccttgac
      241 cacggcgtcc gccagcctcc tggtaccttc gatgccgtcc caggggccgg tttcgtattc
      301 cagggaaatc cggccctgga agttgtgctt gttcagaatc ctgacgcagc gcgcgatatc
      361 cacgtcttcc cagcggtccc agtacttcgc gtggacgatg gtgcgggcgt agggcatgac
      421 gtcgtccagg gcgtggtaga gcatgtactt gtgttcccag ttgcagaagt cggggcaggc
      481 ttccaggcgc gggtggttga cctcgctgat gatgatcctc gtgttgatgg gattggcggt
      541 gatcccccag tggttttcaa tcgtcacgat gacgtcgacc ttgtccccgt attcggccag
      601 ttcgcggtag gagaggatgg ccttttcgag gtacttctcc atcaggtcgt tgcggggcag
      661 gccgttcacc agctccgcgc aggggccgat gaaggggccg gcggtattga cccgcatggc
      721 cttgaccccg atccgggagg cggcgttcat ccacgccttg gcggtggcga tcccggcttt
      781 tcgccgatca tcatccaggt cggccatgtg ctggggaatg tcgttggaga tgtggtagcc
      841 gctgacgccg gtctgcgcga agcggccggc cagcttgtcc agccatgtcc gggaggacgg
      901 ggcgtcgggg tcgaaggcca gggtgtcggt cggcttcccg tcccaccagc tcgtgtgttt
      961 ccggaacatg gcggggtcgg cgatgtcgcc gaacagcccc gccaggagat cgatggaacg
     1021 gatccccggg aagttgtcct tcgtgaactg ggggaaatcg agcagggtga tcgagccgta
     1081 cttcttctgg agttgcctgg cctcgggaga cggaggctgg gcgggattga aggcgtcccg
     1141 gttgaagaca ccccggcagg cgtagcagtt cgacgccagc ctggcccgtt tctcctcgtc
     1201 ggaacggaag cggggagagg cagccagggg cgcgacggcg acggcggccg ccatcgcggc
     1261 tgaggttgtg cggaggaagt cccggcggtt ggcgtcgctc atggctctct cgactcctgt
     1321 gtgactgggg ggcttcttgc gcgaccggca ggatgagcaa gtatgtgtcg ccccggggca
     1381 tcaccgcaag gggaaggcgc tcccttacgc gcgcctcgcg gttctggtgt gccagccgac
     1441 gcgcggagcg gacgctactg gaccgcggcg ccctgcaacc tgaagttggc gtccacgccg
     1501 gtgcctagcg ccggcttgag gtactcgccc gacggcagca gcagccagct ggcctgcgct
     1561 ttcctgaacg gctcggtcgg gtagcccgtc acgtagaggt cgccgcccgt gtacgtgtcc
     1621 ttgttgaaac ccgtcgtcaa ccggccgcgc gtgtcgctgt agaaccagta gcacgtcccg
     1681 ccggtgaccg tcaccgccgc ggggaagatg tactggccgt ccttcacggc ttccgattcg
     1741 gcgaagtacc cgggcgtcgc cggtccgagg ttggagacga ggcccgtgta ctcctgcgtc
     1801 aggatgtaga gcgtcccaaa cgcgacgggc gtgccggccg ggttgtagtg gtaccagttg
     1861 aaccgcaggt tgttgtagga gccagatccc ggaatggtga cggactgccc ggcccagttg
     1921 tccgtgccgg tgtagccggt cgtcacgtct aggaggtcgg cggtcacctc gacgagcggc
     1981 ctgataacga gggccgcccc tccgcccgag aacggcccgc cggacgaggt gggcgcagcc
     2041 ggcgagtcgc cggcggacgt tcgctgacag ccgccaaccg tgacggtcag cgcgacagcc
     2101 gcgacgccaa gcagcacgct ccgaaccgtg agggcttgtt tcatgaacgc ctccggcgag
     2161 cggaacgcct gaggcattat acgcctgcgc ccggagggat gcccggcgcg cctcgcggcg
     2221 cacgtcgcgg tgccggcgcg gcggcccgcg agcgcggtgg acgccacaga cccgagagaa
     2281 cacaataccg tgaccaccag ctgatcgtgc agatgacgtc ccgcccgtcc ctcaccgcgc
     2341 cctcggagcc cgcccgcggc gggttccgct cgcgcctcgc cagctgggcg gacgacgacg
     2401 cgttgcggcg ccacgaggag aaggcgttct tcgtcctgac cctggtcatc ggcgccatcg
     2461 tcggcctggt catcgtcgcg ttcatcctcc tcaccgagaa cctcggggcg cgcctcttcg
     2521 ccccggacgc cgcgagctgg caccggctgg tcatcccggt gctgggcgcg ctggtgtccg
     2581 gcgtcctgct cgcccgcgtc ttccaggggg cccgcggcag cggcatcccc cagaccaagg
     2641 tcgcgctgta cctcgaggac ggactgatcc acctgcgcac cgtgctgggc aagttcgcat
     2701 gctccagcct gtcgctggcg agcggcatcg ccctagggcg cgaggggccg tcggtgcaca
     2761 tcggcgcggg catcgcctcg gtgatcgggc ggcggctcgg cctcagcccg tcacacacga
     2821 agcgcctcgt cccggtcggg gccgcggccg cggtcgccgc cgcgttcaac acgccagtcg
     2881 cggccgtgct cttcacgctc gaggaagtcg tggaggacct gcacgcgccc gtcgtcgggt
     2941 cgattgtcct cagctccgcc accgcctgga tggtgctgcg cctcctgctc ggcgacgagc
     3001 ccctgttcca cgtcccggcg tacgaactgg ttcacccgat cgagttcgtc gtgtatgcgg
     3061 ccctcggcgt ggcaggcggg atcgtgtcgg tggccttcgt gaagctcctg ctggcgcagc
     3121 ggcgatggtt ctgcggcttg ccggcctcga ccgtctggtt ccagccggtc atcggcggga
     3181 tcgtcaccgg cctcatcggc tgggcggtgc ccgccgtgct cggtgtcgga tacgcgcacg
     3241 taggcgaagc gctgaacggc aagatggcgc tcggcgtcat ggcgctgctg ctgggcctca
     3301 aactggtggc gaccgctgcc ggatatgcgt ccgggaatgc cgggggcatc ttcggcccca
     3361 gcctcttcct cggggcgatg atgggcggga gcatcggcgg cctcgtgcac acgtggttcc
     3421 ccgactacac gggcggcgtc ggggcgtacg ccctggtcgg catgggcgcg gcgttcgccg
     3481 gcatcatccg cgcgcccttc acgtcggtgg tcatgatctt cgagatcacg cggaactacg
     3541 ccgtcatcgt gccgctcatg atcgccaatc tcgtgagcta cttcatctcg tcgcgcctcc
     3601 agcgcgagcc catctacgag gccctgctcc accaggacgg cgtccgcctg cccaggcgcc
     3661 gcgccgagcg ggaggatcag ctggccgtcg gtctggcgat gcggcccgcg cgggcgttcg
     3721 tgccggccgg cgagtcggcg gccgccgcgg cagccaggct cggcgtgccg gccggcgcga
     3781 ccgagaccgc gcctgcaggc gcgggtgaag ccggcatcgc ggatccctgg cgccgggggt
     3841 ggcccatcat ggatggcgac tcgttcgtgg gctggctggg cgcagccgat ctcaggcggg
     3901 cgctgcgcgg cggcctccac gacgcgccgg cgcgaggcct cgcggccgcc gtgtctcccc
     3961 cggacccgtc agcgccatcc cgttctctgc atccggacga cacgcttgac ctggccttgc
     4021 ggcgcatggg cgaagcgggg ctcgacgaac tgccggtcgt cagccggacc cgcccggacg
     4081 agttgttggg gacggtgtcg gtgacggacg tggtgggggc ctatcgcaag ggtggcggcg
     4141 atccgcagcc ggaggcgacc cctggtcgaa attcgccgcg cacgctgctg atccggctcg
     4201 ccgtcgtcgt gctgatcgtg ctcatcggaa gcgggatcct cggccaccgc ctgcggacgg
     4261 cgcggctcga gggcgcggcg cgggcgttcg agacaggccg cgcgctcgcg gcgcagggcc
     4321 gtgagccgga ggcaatcgag cagttccggc gggcgctggc ggtttccggg tccgctgacg
     4381 tccggctcga gctcgcgcgg acgctcctcg cagtcgatcg tccggcggaa gccgctctct
     4441 atttcgggga aatgctgcgc gcagacccgg ccagcggcga agcaaacctc ggcctggcca
     4501 gggccgcggg gaaacagggg cggaccgggg aggccatcct ggcctacagc cgcgccctcg
     4561 tcgggaactg gaccggccgt cctgagcagc gccgcatcga ggcgggcttc gagtttgcgg
     4621 actacctggc gcgcagcggg atgcgcgacc aggcggtcgc ccagctgctg gatctgcagc
     4681 aggccgtctc cgggagcccc ggcctgctgc tggacgtcgg gcgccgcttc ctgagcctgg
     4741 gcgccgacgc gcagtcggcc tcgctgttcc gcgcggtgat cacgctcgac cccgcccgga
     4801 tcgacgcgca tctcggcctt ggcgaggccg agctggccca ggacaacttc gaggcggcgc
     4861 gcgacgcgtt ccgccgcgcc gagcagatcg acccgggcaa cgccacggcg gcttcgcgca
     4921 ggcggctgtt gacggaggtg ctctcgctga atcccgagca gcggcgcctg tcgtccggcg
     4981 agcgccaccg tcgaagcctg gcgctgctct cggcggccct cgcgcagttc gacgcgtgcg
     5041 cgaaggcctc gggcgccgcg ggcgccctcg ccccggccgg ccccctcgcg gaggccgcgc
     5101 ggcggacgct cgccagacgg gcccggccgg cgtctctggc ggatgcggcc gaggagaacg
     5161 tcgatgtggc ccagcagctc tggtcggccc gggagcggca gtgcccggca tcgggtgagc
     5221 caacggcagt gggcgtgctg atgaagcacc tcgcccgctg atcggccgtc cgccccgcgc
     5281 cggccgccgc cgtcgatggt cccggcgcgg cgcagacggg cgccatggag aatgcgccac
     5341 gatgaacgtc gtcaacctgg ccgagaagtt cgccttgttc tccgaccact ggcgcccgcg
     5401 catcgtgggt gagctgaatg gccagcaggt caaactcgcc cggctcaagg gcgagttcgt
     5461 gtggcaccat cacgacgacg ccgacgagct gttctacgtg gtgcgcggca cgctccgcat
     5521 gatgctgccg gatggcgagg cgcgggtggc ggccggcgag ttcgtcatcg tgccgcgcgg
     5581 agtcgatcat ctgcccgtgg ccgacgacga ggtgctggtc ctgctgttcg agccggccgc
     5641 gacgcgcaac accggcaacg tcgagaacga ccgcaccgcg accgatctcg agcgaatctg
     5701 aggcaggcag aaggacgcgg ccagcccctc ttcgcgctcg gagcgccttg gccacggcat
     5761 ccgcaagccg cccgctgccc tcgatcccgt cccaggggcc ggcctcgtat tccagggaga
     5821 ccctgccctg gaagttgtgc ttgttcagga tcctgacgca gcgctcgata tccagctcct
     5881 gcctgagccc tcgtcagaat cgcggccacg ggccgtcacg ttccggagaa tggatcgagc
     5941 gccgcggaca accgctgatc ctgcgtgacg agttccgcct cgagcatgcc cgccaagcac
     6001 aggtagctcg cgtcgtacgc cgacaggccc gttgcgttcg cgaccagcac gacatcggtt
     6061 gggtggggat ccagccagac gatgcccgaa cgcagatcga gggtccgttc cagggcctgg
     6121 agcacctcgg cggcctgctc gggctgctcc cggcacttct tcgccgccac ctgctgcagc
     6181 tcgtagcgca gcaacgtcgg agcgtacacg gccgcgccct cgagccggct cgcgctgtcc
     6241 gctccgcccg cctcgtggaa gaccacgccg gccagggcgg acgtgtccac gatgacgcgc
     6301 ctcatcggct gtcgcgatcc tcgcggaccc accgggtcga ccggtccggg cccttcagtc
     6361 cggcagatcg cgcggccgcg agcaactcga acgcgggaaa gggcctggcg ccgacatgcg
     6421 cctcgatcat ggccaacatc tccccctgaa gcgatcggtg gttccttcgc gcccgttcgc
     6481 gcaaggcgtt ggcgagatga tccgggacgt ctttgaccga gagattgact ggcatggaaa
     6541 tggatccaat acgcatccat tatgcatccg ttgtggatcg cgcgtcaaca cgacgccccg
     6601 tgcgactcct cccggttccg gtgtgccagc agcccgaggt cgctgagcat cctcaagccg
     6661 gagccaggcc ggtctcattc acgcctcccg tgagcggaga gccggctgca tcatcccgct
     6721 cctgcgcccg gaggaatgcc cggcgcctcc cgagccgccg tccgccgcaa gacgctcgtg
     6781 cgccgtctcc ggcgaagagc gccatcatct ccacgcaccg agccggcccg tgtgcgcgga
     6841 tgggggcagc gtccgaaggg agtcccggtg aagcagatcc gttcgctgct gtatccggcc
     6901 gcggtcttct tgtgtgtcgc ggggatgccg gagagtcacg ccatctcggc cggggcccct
     6961 ccggccgccg cggtcacgcc tgccgggcgg caaacggcgc cggccgcgag acaggctcct
     7021 ccgaagtctc cggtcaagcc ggtgacgaag cagaaggcgc ctgtcgcggc caacccgctg
     7081 cggccacgat cggcggtgga acttccctgt atcacggcga tgaagccggg aacgcccccc
     7141 tactcgacga ccgggttcat cctcgaggcc ttccggaagg ctcacggcgc gcgcttcatc
     7201 gagtgcgggg tcgggaggat actcctcgac gtcgccagcg gtgcgacacc gcgggcagac
     7261 cgcaggcctg gccctcgcat cgacggttcc aggcgccagg tccctggcgg gcccgctacc
     7321 ggatgcgcgt cgccgaccat ttccacgctc ccacggccgg atcacccgcc atggaatgcc
     7381 caacgggcgt gccggccgtc gctgccccgc ccgccgtccg ggggcggccg cgccggcgtt
     7441 ctccgcgaac ggcggatgat ccccgaggaa gcagtgcctg atgtgcgggt cgggccggcc
     7501 cggcagaaga gtacccggcg acaggattct gcggcagaac agccctgcca aaggagggca
     7561 tgcccgtgaa gacgccgtgt gtgcttgccg gactcctggt tgtggcgctc ggtaccgtca
     7621 ccctggaggg tgcgcaggcc gtggttgccg cagagaagtc gatcgagatc cccaagggga
     7681 cgaaagccga gaagatcggc ccgggccacg tcaggttcac gcttccgaac ctgcgggtca
     7741 tcgacgtcac tggctacgat gcccgcaccg gggcgatcgg cctcgtgcag gtcatcgatc
     7801 cggacccgcc ggnnnnnnnn nnggccaacc gatgcgcgaa ccgccgcgaa gatcatcgat
     7861 ccgcagccgc ccggcaaggt ggagatcacg gcatcccagg ctcgcttggt gaaactgcgg
     7921 gtcctgacca gggcagcggc gaggaagctg cctgccgcgg acgacgtgac catcgacgac
     7981 gagatcacct ggctgccggc acggatcacc gacaaggacg gcgtcaggcg ctgagtcgcg
     8041 ccgcgagccg atcggtccga ggaggttgag atgaagcgag ccagggcgcg gcgcctcgcg
     8101 ccggctttcg cggtcgctgt taccgtcacg ctcgcgcacg gccagtcttc gtacgaacag
     8161 cacctgcaga tccggacgtc gggggtcccg cacgcgggga acggcgtctt cacgaccgtc
     8221 gccattccca agggcgccta cctcggggcc tacacggggg agttcgtcac cgacgacgag
     8281 tacctgcgcc ggtaccaggc caaccggtgg cagtacatga tggggctgct cgactgcgcg
     8341 aagccgcaca ccggcggcct cacgaccatc gacggcatcc gcggcaacgt ggtcacgcgc
     8401 atgaactacg cgccggcgga gttccagaac gtcaggttcc agaagatctg cgagcccccg
     8461 ttcgtgggga tcgtggcgct cagggacatc gcggcgggcg aggaactgtg ggtggactac
     8521 ggcccgaact acccctacga cttcatgaag gacgaggcct tcgtcaagtt cttcgcggac
     8581 ctgcgcgcca cggcgccgac gcacgatcac aggggccgga cgggctccca gatctcgatg
     8641 acgtgcttgt gctccggatg ggccgccgga tcgttgagca tgagctcgaa gcagtgacgg
     8701 tcgtccggct ggtagccgct ggagggaagc cactcgccca tcacccagtt ccaggccgcg
     8761 ccgtattcgc tcgggtcgag ctcgtacgac gcgaccgcgt acaggccgcc ctcgagctcc
     8821 atcacgccgg cgccgccagg gccgtcgccg gccgccgtgc cgggcggcac ggtcaccgcg
     8881 acgctgatgc gcagcttgtc ctcgtccgtg acctccggat tgtcgtgata cacggtcagg
     8941 aaccgcgcgc cgggaccgag caggccgcgc gggcccgccc acgaacagag ccgctcgaac
     9001 agccggccga acagcgcttc gttgcccgcg tagggccccg tgtgccgcac gtacgcgatc
     9061 gtcgtcggct tcagcgtctc gatccggacc gatgcggcgg ccttcttcgg cgtggtgtcg
     9121 tagctcatga tgcgcgctcc ttcttcggcg agaccagcca gcgcggccgg ggcgcatgag
     9181 ccagtgtagg gaacgctccc gggccgctct tcaccatcct tgcggagcgc ttcacgtttc
     9241 ttgcggactt cgcggcgcca ctcggtggcg cttcggccgt aggccgcgcg gaacgcgcgc
     9301 gagaacgccg cggaactgcc gaagccgcag tcgagcgcga tggcggtcag gctcaggtgc
     9361 ggctgccggg cggccttcga ggccgcgcgt tcgagccgca gccgcaggat gaaccggtgg
     9421 agcgtctcgc ccgtgcacgc cgtgaacacg cggtggaagt ggtacgggga gaagttcgcc
     9481 acgccggcca gcgcgtgcag ggacaacgga tcgcccaggt ggtgctcgat gtagtcgacc
     9541 acgcgatgga cgcgcgcaag gtactcggcc cgctgcgagg ctgcggcgga gatgtcggcc
     9601 atggcggcat tctagccccg gcgagcccgg cggcacttgc ggggcttccc ggcatccggc
     9661 cggcctgcag tacaatccga cgacgcacgt cgtgcggcgc cctccaatgc cgccgagtac
     9721 cgggaacggg gtcagacgat gacggaatcc aatcacacac gccgggagtt cctgaagacc
     9781 gttgctgcgg cgggggccgt gtcccttgcc gcgccgcgcg ggctcgacgc ggccaccagg
     9841 cggcgcagca tcggggccaa cgatcgcatc cgcatcggca tcatcggctg cggcgaccgc
     9901 ggcatcggca ccgagatggc cagcgtccac gagcacgccc aggccgagaa cctggaggtc
     9961 atcgcggtgt gcgacccctg gcgtgtggcg cgggaggcgg cggcggccaa ggcgaaggaa
    10021 tggttcggca ccgacgcgag acagtgccgg acccctgacg agctgctgag cctcaaggag
    10081 gtcgatgcgg tcttcatctc gtcgcccgac cactggcacg ccgcgcacct cgaagccgcc
    10141 gcgaaggccg gcaagcacgt ctacgtcgag aagccgatgg cgaacgagat cggggagctg
    10201 aaccgcgcgt acgcggccgc gaaagcctcg ggcgtcgtca tccaggtcgg gacgcagctg
    10261 cgcagctcgg ccacggcgat gggatgccgc gagctgctga agtcgggcag gctcggccgg
    10321 atcacgcgca tcgagcaggt gcgcaacagc gacaagccgt actggtacca gtacctgaaa
    10381 cccgacgtgc gggcggccga tctcgactgg caggccttca ccaagggccg gacgaagaag
    10441 gccttcgatc cgatcctgta ctcaggctgg tacggctact gggagttctc ccagggcccg
    10501 gtcccgcagt ggggcgtgca cttcctcgac ctcgtgcact tcatcgccgg cctcggcatt
    10561 cccgacacgt gcgtgtgcct gggcggggtc tacacgtgga aggacgagca ccgcttcacc
    10621 gcgccggacc aggtgcaggc gctctggcag tatcccgaag gcctgatgat cagctactcg
    10681 accaacttcg ggaacagcgc cggcaacctg acccggttca gcggcgacaa gggcacgctc
    10741 cggctcgact ggtcgaacgc ggtgtacagc gccgagggcg gcatcaaccg cgacggctcg
    10801 atccgcggcg agaacaaggt ggagccgatc gagcagacgg accactgggt gaactggtac
    10861 cggtgcatgc gcgacggcgc cacgccgaac gcgccgctcg acgccgggta ccagcacgcc
    10921 gtggcgagca tcatggccac catgtcctac gagacgggcc gccgcgcgcg gttcgatcgc
    10981 gcgaggcgca cgatcgcgac cgactgaggc tcacgccggg cgccgtcacg ctcccagttc
    11041 gcggtacagc gcctcgtact gcggcacgat cgcgtcggcg cagtagcggg aggccaccgc
    11101 ccggcggcct tcgcgggcca tcgcggcgtg cgcggcccgg tcgctcaaca ggcggatcga
    11161 gctggcggcc atgccgtcga gatcgtccgg cggatggagc atgccggtga cgccgtgcgt
    11221 gaggacctcc ggcagcccgc ccacgctgga cgccacgacg ggcaccttgc acgccatcgc
    11281 ttcgagggcc gccaggccaa agctctcctg caatgacggc agcaggaaca ggtcggcgac
    11341 cgacagcagc ggcacgacgt cttcctgcgg cggcaggatc tccacgtcgc gcccgagccc
    11401 gtgcgtctcg atttcgtcca gcaccttgac gccgtccggc ccgtcgccgg ccaggaccag
    11461 gcgggcccgg atgcgctcgc ggatccgcct gaacacgccg accaccgcgt ccacccgctt
    11521 gacgggccgg aagttggaca ggtggacgac gagggcgtcg tagtccgcct gcggacagag
    11581 ccgcccgcgc aggccggcgt cgaacacgcg cctgaaccgc tcgcagtcca ggaagttcgg
    11641 gatgacgcgg accggcgcgg ccgtccccaa acgctcgatc gtgtcccggc gcaggctctc
    11701 cgagaccgcc gtcacggcgt ccgaccgatc gatggagaac ctcaccgtgt cggcgaacgc
    11761 cgggttgctg ccgatgagcg tgatgtccgt gccgtgcagg gtcgtgatga cggccgggat
    11821 ccgcccgccg cactccgtcc gcaggatttc gcgggcgagg taggccgccg tcgcgtgcgg
    11881 cacggcgtag tgcgcgtgga tgacgtccag ctgccgctcg cgggccgtct gcacgatccg
    11941 cgtggccagc gagatcacgt actgcggctc gtgaaacagc gggtagtcgg gcgtctcgac
    12001 cgtgtggagc acgacgccgc cgggatcggc gcgcagcctg aacgggatgt cgctgctcag
    12061 caggtgcgcc tcgtggccgc gccgcgcgag gtgccgggcg agctcggtgg cgacgatgcc
    12121 gctgccgccc agcgaggcgt agcagacgat accgaccctc atcggcggcg ccgggctccg
    12181 ggccagcgat ccgtgagcag cgggagcacc accggctccc tgaccgagaa gccctccgcg
    12241 tacctgacgc cggccgctgc tccgaactgc gcgtcgcggc tctcgacaag ctgcaggaac
    12301 agcggcgagg tgaggcgagt ggaggcggcg tcgggcgcct cgggccggaa ctggctcgcg
    12361 tgacacgcca gcgcccgccg cttgaggtcg tagacgtccg acacgtccac cacgaaggac
    12421 ggcggcgcgc tgtcgttgat gaagtacgcg cacgcccagc gggcgcgcca gggcgcgcca
    12481 gaggcggggt agcgccgcag gccggccttg aagatcgccg actcgagcag cgccgcagcg
    12541 cggcggtggt cgggatggcg atcgtcggcg taggggaccg cgatcgcggc cggcctggcg
    12601 ctccgcacga gatcgacgat cgcgccgacg tgaggttccg tgtcggcgag cgccccgtcg
    12661 ggcaggccca ggttcaggcg ccaggccgcg ccgagcaggg ccgcggcggc ctctccctcg
    12721 gccacccgct cttccggcgt gccgttgctg cccagctcgc cccgcgtcag gtcgcacagg
    12781 cccacgcgcc agccgaggga cgcgtgcttg gcgatggtcg cggccattcc gatctcgatg
    12841 tcgtcggggt gagggccgaa ggcgagcaga tcgacggggc tcggagcggt catggccggc
    12901 tgtctcgatc ctacaccgga gccggccccc agcgcgggcg gctcgccggc ggccgggatt
    12961 cccgggcgcc ggctgccgca gcagggctat catcttcacg ccgggagcca cgatccgccg
    13021 cggcgcgccg ccgggcgcga gagccggcac gcaggcgccg cgcgcgccgg ctcggggagg
    13081 gtggaccgat gcgaagcagt gtgcgccgcc tcctgccgcc cgtcgtggcc gtgcttgccg
    13141 tcgcgttcgc gcacggccag acgtcctacg agcggcacct cgaaatccgg ccgtcgagga
    13201 ttccgcacgc gggcaacggc gtcttcacga cggcggccat ccccaagggc gcccgcctcg
    13261 gggcctacac gggcgagttc gtcaccgacg aggagtactc ccgtaggtac caggcgaacc
    13321 ggtggcagta catgatgggg ctgcgcgact gcgcgaagcc gtacaccggc ggcatcacga
    13381 ccatcgacgg catcgccggc aacgtcttca cgcgcatgaa ctacgcgccc gcggagttcc
    13441 agaacgtgaa gttcgagaaa acgtgcgagc ccccgttcgt gacgatcgtg gcgatccggg
    13501 acatcgcgcc gggcgaggag ctgtgggtgg actacggccc gaactaccgc tacgacttca
    13561 tgaaggacga ggccgtgatc gagttcttcg cccgcctgcg cgcggcgcgc gcgcgacggc
    13621 cgtagaacgc gggcgccggc gcggcccgcc ggcgtccgcg ccgccgaagg atcgcggggc
    13681 cggccgccgg cgtcttaccg gatgaggcac gggatgacga tcgccttttt cgagctcgaa
    13741 gggtgggagc gcgccgcgct ggcggagcgg ctgtcggaac acgatctgct gttctacgag
    13801 gacgtcctgt cgccggcgca cctcccggcg ctcgcggcgt gcgcggtggt gtcgcccttc
    13861 atctattcca ggatcggccc ggaggcgctc gcggccatgc cggccctgaa gatggtcgcc
    13921 acccgatcca ccggcttcga tcacatcgac gtcgccgcct gccgggcgcg cggcattgcc
    13981 gtgtccaacg tgcccgagta cggctcgcag acggtggccg agcacacctt cgcgctgatc
    14041 ctggccctct cccgcaagct cgtcccggcg taccgggcca tgcggaacac cgagccccgg
    14101 tccgacgagg cgcggcggct gcgcgggttc gacctccgcg gcaagaccct gggcgtcgtc
    14161 ggcgccggca acatcggcct ccacgtcgtc cgcatggccg agagcttcgg catgcgcgtc
    14221 ctcgtgttcg acacgcggcg ccgccagacg ctcgcggacc tcctcgaatt cgagtacgtc
    14281 ccgctggagg cgctgctccg ggagtcggac atcgtctcgc tgcactgccc ggccacgaac
    14341 gagacgcggc acatgatcaa tgccgacagc ctggcgatca tgaagcgcgg cgcgctgctc
    14401 atcaacacgg cgcgcggcga gctcgtggac acggccgcgc tgctcgctgc gctgcagtcc
    14461 gggcagctcg ggggcgcggg cctggacgtc ttcgagggcg aggcgatcgt gcgcgaagag
    14521 gcgcagatcc tgtccagggc cttcgaccgg gagcagctcg agagcgccat ccgcgcccac
    14581 cagctcctca agcgcgacga cgtcatcgtc accccgcaca acgggttcaa cagccgggag
    14641 gccgtcgagc gcatcctcga cacgaccgtc cggaacatcc agggtttcct ggcgggcgtc
    14701 acccagaacg cggtctgaac cgccccgccg cgatcggatg ccggtccggc tgatgggccg
    14761 gggagggcga accggctttc gaagaccggg gcgacggccg tccttctcgc gctgctgtcc
    14821 cgcgccacgg ccgccgcggc gagcgtgaag ggcgtcggca tggccgcgcc ccgcgccggc
    14881 gccggggtgt tcgtggggat tgccgggacg gcctggctga gagcgtatct gcgtcccgcc
    14941 tgcctcggtg tgcgacgtgc gtcgtgcggg cccgggccct caccgatgac gtgaaggcga
    15001 ggatggactc gcggcgcgtc cggacgcgcg catcagccgg ctggccgaaa gctggcgtcc
    15061 ccgacgggat tcgaacccgt gttttggcct tgaaagggcc acgtcctaac ccctggacga
    15121 cggggacacg aacggggcgc gggagcaccg ccgaccgtga gaagaaggcg gcgaacactc
    15181 gatagtacca tacgcctcgc cgcgcccgcc accagtcagc gcacctccgt ccctactgtc
    15241 ggcgcatccc acaggaagcg ccaacggcct cacgctcgcc ccgcaagccg gcccgtcagt
    15301 gcctcatccg acggccacgc cggaccttcg accggcggtg cagcgagcgc gcggtgccag
    15361 cccaggggcc catcgacgcc gtcggggtct tcttgccg
//
LOCUS       DAOSIF010000015        15355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4001_length_15355_cov_4.802549, whole genome shotgun sequence.
ACCESSION   DAOSIF010000015 DAOSIF010000000
VERSION     DAOSIF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15355
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4001_length_15355_cov_4.802549"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PLT35_01255"
     CDS             complement(<1..975)
                     /locus_tag="PLT35_01255"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOG27880.1"
                     /translation="MAMSAPRRFKPYPAYKDSGVEWLGEIPAHWEVKRLKYAAPARVS
                     KLEVKPGDAVYVGLEHIESWTGRLLLENQPETVDSVVGCFKAGDVLFGKLRPYLAKVA
                     RPDFDGVCTSEILPLRPAAECSQSYLMYELLNAPYIHWLDSLTYGTKMPRVSPEQVAV
                     SFVSVPPLPEQRAIAAFLDRETARIDALVAKKERLIELLQEKRTALITRAVTRGLDPT
                     VPMKDSGVEWMGEISAHWEVKRLRHIGQAIIGLTYQPDDVVGPEEGVLVLRASNVRDG
                     KVVYEDNVFVRSAIPERLRTQLRDIVICARSGAMSRRLLKFSGGSSDGY"
     gene            complement(966..1883)
                     /locus_tag="PLT35_01260"
     CDS             complement(966..1883)
                     /locus_tag="PLT35_01260"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG27881.1"
                     /translation="MTRSPEDNLAQSIQARLKNAAIEGGRPFVELLELYAVERFLHRL
                     GRSRQRDRFVLKGAMLLRQWLGAQSRPTRDIDLLGPVDLDATALRAAIAEILSTEVED
                     DAIVFVEDSIAVRPIRGQSAVLGLRAKFDATLGRARLRYQVDVGLGDAVFPPDVEIIP
                     GGLLAMPMASVRAYTPYSTLAEKLEAVVVLGNANSRTKDYYDLFQLPRALSLEGDLLA
                     ESIRRTFARRATPVPPTPLEGLSDAFARDPLHAGRWRAFLDKGHLDVAEEDFVRVVAA
                     IRNFASPVLDAVRAGSSFDRQWPAGGPWR"
     gene            complement(1880..2488)
                     /locus_tag="PLT35_01265"
     CDS             complement(1880..2488)
                     /locus_tag="PLT35_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOG27882.1"
                     /translation="MNGSDRAARAREAFHRAGGVLRTAQAVRAGIHPRDLYALRDAGA
                     LEVVSRGVYRLADLPPLAEPDLAAVSARIPKAVIAVVSALHFHGLTTEIPHAVSIALP
                     KGTSRPRLEWPPLRVYWFSGERYTEGIETHARDGVTLKVYGAARSVADCFRLRRRLGI
                     EVAVEALRAGLEERIFTPAEILRVAERLRVAGVVRPYLEAVQ"
     gene            complement(2627..4783)
                     /locus_tag="PLT35_01270"
     CDS             complement(2627..4783)
                     /locus_tag="PLT35_01270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOG27883.1"
                     /translation="MNHSEIVSFLWGVADLIRDTFKRGKYQDIILPLTVLRRLDCVLA
                     DTKQKVLERQGQLKGKGLEDLDAQLRLASGFAFYNTSRYDFEKLLADAPHLAANLRNY
                     IAGFSPNMREVLEKFDFDNTISKLDEAGLLFQVLERFKNVDLHPDRIDNPTMGTIFEE
                     LIRKFNEALNENPGEHFTPRDVVHLMVDLMLAGDSDRIRRRGIVCTVYDPCCGSGGML
                     MITKEHITAGLRKNGGPLRPAINTDAEIHLFGQEVNPETWAVSKSDLFMKDPTGRDAD
                     NIAYGSVLSNDRHAGRSFDYLIANPPYGKDWKRDEDAVRAEHERGASGRFAPGLPRIS
                     DGQLLFLLHMLAHAKEPKEGGSRIAIIMNGSPLFTGDAGSGESEIRRFILEHDLLEAL
                     IALPEQLFYNTGIATYVWVVTNRKAPARKGTVQLIDATSFWVPMRKSLGDKRREIPPE
                     KAQDIVTLLAGFKDGETRRVTKDGTEEEVVVSRIFPATHFGFRKITVERPLRLNFQAS
                     AERIARLEEEKGFQELAQSKKKGAAGAKEQAEGRELQAAIRALLRGLPGTLFKDRNEF
                     EEALDAVARKAGQKLPAPARKAILSALGERDETAAICRDRDGNPEPDPELRDTESVPL
                     PAGDDPADAEGVPASVRAFLEREVKPHVPDAWIDTRKRDPRDGRVGLIGYEINFNRYF
                     YRYTPPRPLEEIEADIRAIEKDIVRMLAEVTGTAKV"
     gene            complement(5415..5873)
                     /locus_tag="PLT35_01275"
     CDS             complement(5415..5873)
                     /locus_tag="PLT35_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27884.1"
                     /translation="MLEDALVRALTDDEAALVTSADFTRWAQQRNVKAGAPVRSCHFT
                     RQLQAQFASLVEPGQVKKLAGLRLRSRMKLIWLLLPFTLICSALVAGGAAAQVPPLPG
                     GAQTHWVVGQPHEIVAYVVFDPATVERTRGDSRGEPVRVGESGTSRGSDR"
     gene            complement(5994..8264)
                     /locus_tag="PLT35_01280"
     CDS             complement(5994..8264)
                     /locus_tag="PLT35_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOG27885.1"
                     /translation="MPQLVAAVSRLAVLVLFVLFLPASAWAQAGPPGVAALALLPVPA
                     TVVQLEGSLAVDEHFTVVLEGPADPIVAGAVERFLTRLQARTGLPVRLTPLRSGPGTL
                     RIRWGVASGPGADPFADERYTLDVGPDGARLEAPAAGGVLRGLATMQQLVRVGADGIR
                     ARAVHVEDAPRFSWRGLMLDVARHFVAKDEILRTLDAMESVKLNVLHLHLSDNEGFRV
                     ESRRYPRLTAYGSSGGQFYTQADIRAIVAAARDRGIRVVPEIEMPGHVQSILVAYPEL
                     GSSSKPMARGRRPDTMLAALDPSREETYRFVAGLLGELAGLFPDASIHIAGDEVTGAQ
                     WLENPAIRAFMRRRGFESAHELQAYFTRRVAAIAVALGKTPTVWDEALHADLPPGVVV
                     QAWRSSKMVHRATAAGHRTIVSAGYYLDHGLPAARYYQVDPFDTRATGMAAEMLATLK
                     GTPLEAYVTEAMVASDSPELTEAGQRLVAGGEAAMWTELVPDGHVEMHAWPRAAAVAE
                     RLWSPAAVRDTNDLLRRLELISTDLELQGLQHRAAHVRALRRLAAGGDLAPLATLAEA
                     VEPLKNLARLMPIIQSAMAGPASTAYYPPMNRFADALPPESVAARRFNDAAAGAGSKP
                     EERAFLRAKLQAWRDNHAAFTRSAERSSLLVEMVPVSADLRTLAEAGLEALDALDAGA
                     PLAPARAEALRGLMAPYLGAEAATANVIMALMAPQPTHGVSIAIIRGISALVEAASPR
                     AGSRGGLPFVEPSGAS"
     gene            complement(8837..8910)
                     /locus_tag="PLT35_01285"
     tRNA            complement(8837..8910)
                     /locus_tag="PLT35_01285"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8876..8878),aa:Gln,seq:ctg)
     gene            9053..10009
                     /locus_tag="PLT35_01290"
     CDS             9053..10009
                     /locus_tag="PLT35_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG27886.1"
                     /translation="MSSAPPTIAIRAANLTRRYGDILAVDDLSLEVRRGEILGLLGPN
                     GAGKTTTLRMLCGLLRPDSGRVEIDGRPLKAGRPGGLPAIGLSPQAIVIWELLTVFEQ
                     LDFMGRAYGLPRRAARARAEALLTAFGLEEKRDRLGRTLSGGLQRRLNIALAFAHDPP
                     IVLLDEPQAGLDPQSRVLVRDYIGSLRGRVTVIVTTHDMDEAERVADRVCIVDRGRIL
                     ALDTVDAIKGRLGEGDVIEAEFGGADAAALDALAAALARGGRPCARRGTCLRFTSAEG
                     FSAMADLLQAASALGLQARRLAVRKQTLEDVFIRLTGRGLRE"
     gene            10006..11169
                     /locus_tag="PLT35_01295"
     CDS             10006..11169
                     /locus_tag="PLT35_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG27887.1"
                     /translation="MRMLACLRKTLVENVRDWKILILTLSFAPFFVYLMYGYLHTAPA
                     SYTLLVLNDDRAASPPRDAAGADGLIAAWTRAGGDDGRPVFAVRAAGDRQAAERMVKT
                     READLLVVIPAGFSARLAALRAQPAGPPPRLTNHHNAANPRSMMAMAMSDYVAFLYAS
                     AAASMPSPLAVDARAVDPLPNRSDFDLYVPALLVLAVIMVLFTAAASLIREVDKGTMT
                     RLTLSRVATWELLAAVTITQVLIGAATVLLAYAAASSVGYRALGSPGAALAVTTLATV
                     SVVAIGVLVSAFMRTVFELLTVGCFPFFILMFFSDVMIPLPRIPVLSVAGNAVNLSDV
                     LPTSLAVRALNRVLNDGAGLGDVCFEMTGMALLAAAYFSAGILLFRRRHLRTN"
     gene            complement(11221..12774)
                     /gene="gltX"
                     /locus_tag="PLT35_01300"
     CDS             complement(11221..12774)
                     /gene="gltX"
                     /locus_tag="PLT35_01300"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG27888.1"
                     /translation="MSTPRLRFAPSPTGYLHVGGARTALFNWLYARKTGGAFVLRIED
                     TDVERSSADMVTGILDGMRWLGLDWDEGPEAGGAHGPYFQSQRLARYRELAEQLVREG
                     HAYYCYCGTDKERPDGPAFAGDARAAASGGGSAADREGGEGGTPRGESWTYDRRCSRL
                     TPDEVAAREAAGAPRAIRFRVPEGETAFRDLVHGDITFDNAHIEDFVALRSDGHPTYH
                     LSVVADDIDMAITHVVRGDDHISNTPKQVLLYRAFGKPLPAFAHVPLILGPDKRRLSK
                     RHGATSVTEYRRQGYLPEAMMNFLALLGWSPGSDQELFTRDELVAAFSLEGISLSNAV
                     FNPEKLEWFSAQHIARMPAPELVRRVEPLLEEAGLWPVRPGPESPGASAPGPALAWLA
                     RLVELLRPRAKRLTDFVEKGRPFLVEHIEYDAAAVQKHLSSPDARAHLAAVGEACASV
                     EPFAAAALEPAIRALAGSRGVKAGALVHPTRVAVVGRAESPGLFEVLELLGRERVLAR
                     IGRALRLGA"
     gene            complement(13087..13180)
                     /locus_tag="PLT35_01305"
     tRNA            complement(13087..13180)
                     /locus_tag="PLT35_01305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13144..13146),aa:Ser,seq:gct)
     gene            complement(13210..13626)
                     /locus_tag="PLT35_01310"
     CDS             complement(13210..13626)
                     /locus_tag="PLT35_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27889.1"
                     /translation="MPEELKPEAPPLVAATPPAAPPAAEPAAPSPLEAVKAVAVKAAE
                     NVKEAAPKARKAVKKAVKKPAKKAKKAVKPVKRAVKKAAKPAKKAVRKAAKPAKKAVK
                     KVAKKAKKAVKPVKRAVKKAAKPVKKAAKRAGKGRK"
     gene            14009..>15355
                     /gene="glmM"
                     /locus_tag="PLT35_01315"
     CDS             14009..>15355
                     /gene="glmM"
                     /locus_tag="PLT35_01315"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG27890.1"
                     /translation="MRPLKIGISGVRGIVGESLTPELIVSFAQGFGTYLDSGRILVCR
                     DTRASGPMVHAAVVSGLLAAGCEVVDLGVCPTPSLQHAVPWLGAQGGISISAGHNPSV
                     WNALKFVRNDGLYLNAAQAEELLDIVHQGEFEKAAWDRIRTGVPVQDAVGHHLEVLSG
                     AFNLDAALRRALKVAVDCCNGASTFLSPRWLEALGCEVLAINDDPEAPFPHRPEPKPE
                     MMAQLKAVVRAGRADLGFAHDADGERIGLVTGDGEALSEERTLTLAAAIALSHTPGVV
                     VTNISTTRAVDDVAGRFGGSVVRTPVGQSFITEAMIEHRAVLGGEGNGAVAVPRVLMS
                     HDAAATQGLVVEHMAVTGASLRELVDALPCYEMVKRDFALAPGALYSRLQDFRDRIER
                     EGLPANTIDGAVVSFTDGWVHVRASQTESMIRVIAEAASRERAQDLVDWARDRLGTG"
BASE COUNT         2236 a         5433 c         5276 g         2410 t
ORIGIN      
        1 gtaaccgtca ctcgagccgc cgctgaattt caacagccga cgggacatcg ccccgctccg
       61 tgcgcagatg acaatgtcgc gaagctgagt tcggaggcgc tcggggatgg cgcttctaac
      121 gaatacgttg tcctcgtaga caacctttcc atcgcgtaca ttcgacgccc ggagcaccaa
      181 tacgccctcc tctgggccga ccacatcatc cggttggtat gtcagaccaa tgatcgcctg
      241 gccgatgtgc cgtagtcgtt tcacttccca atgcgccgaa atctccccca tccactcgac
      301 gcccgagtcc ttcatgggga cggtcgggtc gaggcccctg gtgacggcgc gggtgatgag
      361 ggcggtgcgc ttctcctgga gcagctcgat cagccgctcc ttcttcgcca ccagcgcatc
      421 gatccgcgcc gtctcccggt cgaggaacgc ggcgatggcg cgctgctcgg ggaggggggg
      481 gacactcacg aaactgacgg cgacttgctc ggggctgacg cgaggcatct tcgtgccgta
      541 cgtcagcgaa tcgagccagt ggatgtacgg cgcgttgagc agctcgtaca tcaagtagct
      601 ctgggaacac tcggcggctg gccgaagcgg gaggatctcg cttgtgcaaa caccgtcgaa
      661 gtctgggcgt gccactttgg cgaggtacgg acgcagcttc ccgaacagga cgtctccagc
      721 tttgaaacac ccaacgacgc tgtccacggt ctcgggctgg ttctccagca gcagccggcc
      781 cgtccacgac tctatgtgct cgagtcccac atagacggca tcgcccggct tgacctccag
      841 cttactgacc cgagcagggg cggcatactt cagccgcttc acctcccaat gcgccggaat
      901 ctcccccagc cactcgacac ccgagtcctt gtaggccggg tacggcttga accgccgcgg
      961 cgcactcatc gccatgggcc tccggccggc cactgccggt cgaaggaaga ccccgcccgg
     1021 actgcgtcga ggaccggtga tgcaaagttc cggatggctg cgacgacgcg cacgaagtcc
     1081 tcctcggcga catccaggtg gcccttgtcg aggaaggcgc gccaccgccc ggcgtggagc
     1141 ggatccctcg cgaacgcatc gctcaggccc tccagcggcg tcggcgggac gggcgtggcg
     1201 cggcgtgcga aggtacggcg gatcgactcg gcgagcaggt ctccttccag gctgagcgcg
     1261 cgaggcagtt ggaaaaggtc gtagtagtcc ttcgtccggc tgttggcgtt gccgagcacc
     1321 acgacggctt ccagcttctc cgcgagcgtg gagtacggcg tgtaggcgcg cacgctggcc
     1381 atcggcatgg cgagcagtcc gcccgggatg atctcgacat ccggaggaaa gaccgcatcg
     1441 ccgagcccca cgtccacctg gtaacgcagg cgtgcgcgtc ccagcgtggc gtcgaacttc
     1501 gcacgcaggc ccagcaccgc ggactgtccg cggatcggcc gcaccgcaat cgagtcctcg
     1561 acgaacacga tcgcgtcgtc ttcgacctcg gtgctcagga tctcggcgat ggccgcgcgc
     1621 agcgcggtgg catcgaggtc gacgggaccc aggaggtcga tgtcgcgcgt gggccggctc
     1681 tgcgctccga gccattgcct cagcagcatc gcccccttga ggacgaagcg gtcgcgctgg
     1741 cgcgaacgac ccaggcgatg gaggaaacgc tcgaccgcat agagctccag cagttccacg
     1801 aacgggcgac cgccctcgat ggcggcgttc ttcagccggg cctggatcga ctgcgcgagg
     1861 ttatcctccg gtgaccgggt cattgcacgg cttccagata ggggcgtacg accccggcaa
     1921 cgcgaagccg ctcggcaacc ctcaggatct cggcgggcgt gaagatccgt tcctcgagcc
     1981 ccgcccgcag cgcttcgacc gccacctcga tgccgagacg tctccgcagc cgaaagcagt
     2041 cggctacgct gcgggccgcc ccgtagacct tgagcgtcac gccatcgcgc gcgtgcgttt
     2101 cgatgccctc ggtgtagcgt tcgcccgaga accagtagac gcgcagaggc ggccattcga
     2161 gccgcggccg gctcgttccc ttcggcagcg cgatgctgac ggcgtgcggg atctccgtcg
     2221 tgagtccgtg gaagtgcagc gccgagacga cggcgatcac ggctttcgga atgcgcgcgg
     2281 agaccgcggc gagatcgggc tcggccaggg gcggcaggtc ggcgagacgg tagaccccgc
     2341 ggctcacgac ctcgagggct cctgcatccc gaagcgcata caggtcgcgg gggtggatgc
     2401 ccgcccgcac cgcctgggcg gtgcggagga cgcctccggc cctgtggaag gcctctcggg
     2461 cgcgcgctgc tcggtcgctc ccgttcactg gatagaaagt acattacttg agggtagata
     2521 tcaagatttc tagccagccc ccggaatggt tcggtcggtc ttctggagac ggttgaaccg
     2581 gcgacacaag tcgtcggcac ttgcgcagat gtgccgacac cttgcgtcaa accttcgccg
     2641 tccccgtcac ctccgccagc atccgcacga tgtctttctc gatcgcccgg atgtcggctt
     2701 cgatctcctc cagaggccgg ggcggcgtgt agcggtagaa gtagcggttg aagttgatct
     2761 cgtagccgat gaggccgacg cggccgtccc tggggtcgcg cttcctcgtg tcgatccagg
     2821 cgtccgggac gtggggcttc acctcgcgct cgaggaaggc gcggacgctg gcgggcaccc
     2881 cctcggcgtc ggccggatcg tctccggcgg gcagcggcac gctctcggtg tcgcgcaact
     2941 cgggatcggg ctcggggttg ccgtccctgt cgcggcagat cgcggccgtc tcgtcgcgct
     3001 cgccgagcgc ggacaggatc gccttgcggg ccggcgccgg gagcttctga cccgccttcc
     3061 gagcgacggc atcaagcgcc tcctcgaact cgttgcggtc cttgaacagc gttcccggga
     3121 gcccgcgcag aagtgcgcga atcgccgcct gcagctctcg gccctcggcc tgctccttcg
     3181 cgccggccgc acccttcttc ttcgactgcg cgagctcctg gaagcccttc tcctcttcga
     3241 gccgcgcgat gcgttcggcg ctcgcctgga agttgagccg cagcggccgt tcgacggtga
     3301 tcttgcgaaa gccgaagtgc gtggccggga agatgcggct gacgacgacc tcttcctccg
     3361 tgccgtcctt cgtaacccgg cgcgtctcgc catccttgaa cccggcgagg agcgtcacga
     3421 tgtcctgcgc cttctccggc gggatctcgc gccgcttgtc gccgaggctc ttgcgcatcg
     3481 gcacccagaa cgaggtcgcg tcgatgagct gcaccgtgcc cttgcgtgcc ggcgccttgc
     3541 ggttggtcac cacccagacg taggtggcga ttcccgtgtt gtagaagagc tgctcgggaa
     3601 gcgcgatcag cgcttcgagc aggtcgtgct ccaggatgaa gcggcggatc tcgctctcgc
     3661 cgctgccggc gtcgccggtg aagagcggcg agccgttcat gatgatggcg atgcgcgatc
     3721 cgccctcctt cggctccttc gcatgcgcca gcatgtgcag gagaaagagg agctgcccgt
     3781 cgctgatccg cggcagcccc ggcgcgaagc ggccggaggc gccgcgctcg tgctcggcgc
     3841 gcacggcgtc ctcgtcgcgc ttccagtcct tgccgtaggg aggattggcg atcaggtagt
     3901 cgaaagaccg gccggcgtgc ctgtcgttgg agagcacgct cccgtaggcg atgttgtccg
     3961 catcgcgccc ggtcggatcc ttcatgaaca ggtccgactt cgagacggcc cacgtctcgg
     4021 gattcacctc ctggccaaag aggtggatct ccgcatccgt gttgattgca ggccgcagcg
     4081 ggccgccgtt cttgcgcagg cctgcggtga tgtgctcttt cgtgatcatg agcatgccgc
     4141 cggacccgca gcacggatcg tagaccgtgc acacgatgcc cctgcgccgg atgcggtcgg
     4201 agtcgccggc cagcatcagg tccaccatca ggtgcaccac gtcgcgcggc gtgaagtgct
     4261 cgccggggtt ctcgttcagc gcctcgttga acttccggat cagctcctcg aagatcgtcc
     4321 ccatcgtcgg gttgtcgatc cggtccgggt gcaggtccac gttcttgaac cgttcgagca
     4381 cctggaagag caggcccgcc tcgtcgagct tcgagatcgt gttgtcgaag tcgaacttct
     4441 cgagcacctc ccgcatgttc ggactgaagc ccgcgatgta gttgcgcaga ttggcggcca
     4501 ggtgcggtgc gtccgcaagc agcttctcga agtcgtagcg ggaggtgttg tagaaagcga
     4561 agccggacgc gagccgaagc tgcgcgtcga gatcctcgag ccccttgccc ttgagctggc
     4621 cctggcgctc gaggaccttc tgcttcgtat ccgcgagcac gcagtcgagg cggcggagca
     4681 cagtgagcgg caggatgatg tcctggtact tgccccgctt gaaggtgtcg cggatcaggt
     4741 cggcgacgcc ccagaggaag ctgacgatct cgctgtggtt cacgtgctca cctttcagcc
     4801 gctcgtgtca ccgcgtcgcc tggttggggc cgtccagcgc ccggcatcgg cggcggcccg
     4861 aagcgccgtc ggccgccggg acggccattc gttgtcggtc cggcgctcga actcgcgcgc
     4921 gaaggcctcg cagatgcgct ggagccctga acgggaagcg cgctccagcg cctgtcgccg
     4981 cttcccgtct ccagtcggca aatgatactc gtctttcgag agcgccagaa cgacatgcgg
     5041 gaacccaacg gcacccggga aatcgcagat cgcgtcgaac atcgttgggc tcgcttcagg
     5101 aagcgggcat ggccagaagc ccgtccttcc atctcagaac tcgacaatcc gtggcattga
     5161 gacgagcgga gcgggaagaa gacggacggc gcgccgaaca gggacagcgg agccactgct
     5221 ccacctgtga gcccgaaagg tatccgtcac ggacagggac agcgcccgcc tgtcggcctc
     5281 ttcggcaagg ggtagtctcg cccttcgacc tgttggtgtg cgggaacgaa accgaggatt
     5341 ggctgggtgt cagtgacgcc ttccgcaact gggtcatggc gaacgttgcc tgacctgctt
     5401 cgcaggggta ccctttatct gtccgaacca cgggaagtcc cgctctctcc aacacgaacc
     5461 ggctcgcctc gcgaatcacc gcgcgtacgc tccaccgtgg cgggatcgaa gacgacatag
     5521 gcgacgatct catgcggttg acccaccacc cagtgcgtct gagcacctcc gggaaggggt
     5581 ggcacctgcg cggcggcgcc tccagcaacc agcgccgagc agatgagcgt gaacggcaac
     5641 agcagccaga tcagcttcat gcgcgaccgc aatcgaagcc ccgcgagctt cttcacctgc
     5701 cccggctcca ccaggctggc gaactgcgcc tgcaactggc gcgtgaaatg acaactgcgc
     5761 accggcgcac cggccttcac gttgcgctgc tgcgcccacc gagtgaagtc cgccgacgtc
     5821 accagcgccg cctcgtcgtc cgtcagcgcg cgcacgagcg cgtcttcgag cactcgcttg
     5881 accgcatcgg cctagccggc cggcgtttgc ggacgggcgc cgttttcatg gcggtgctca
     5941 gcgaatccca cgaacttcca cccggtcctg gttttggccg atgcgggccg tgctcaggac
     6001 gccccacttg gctcgacgaa tggcaaccct cctcgactcc ccgcccgtgg cgatgccgcc
     6061 tccaccagcg cggagatgcc gcggatgatc gcaatcgaca cgccgtgcgt gggctgcggc
     6121 gccatcaacg ccatgatgac gtttgcggtg gcggcctcgg cccccaggta cggcgccatc
     6181 aggccgcgaa gcgcctcggc ccgcgccggc gcgagagggg cgccggcgtc gagggcgtcg
     6241 agcgcctcca agcccgcctc cgccagcgtc cgcaggtcgg ctgacaccgg caccatttcg
     6301 acgagcagcg aggaacgctc ggccgagcgg gtgaaggccg cgtggttgtc gcgccacgcc
     6361 tggagcttcg cgcgcaggaa ggcccgctct tccggcttcg aaccggcccc ggccgcggcg
     6421 tcgttgaatc ggcgggccgc gacgctctct ggtggaaggg cgtcggcgaa ccggttcatc
     6481 ggcgggtagt aggcggtgct cgccggcccg gccattgcgc tctggatgat cggcatcagc
     6541 cgcgcgaggt tcttcagcgg ctccacggct tcggcgaggg tagcgagggg cgcgagatcg
     6601 ccgcccgcgg cgaggcgccg gagcgcacgc acgtgcgcgg cgcggtgttg cagtccctgc
     6661 agctcgaggt cggtcgagat cagctcgagg cggcgaagga ggtcgttggt gtcgcggacg
     6721 gccgccggcg accacagccg ctcggcgact gccgccgcgc gcggccatgc gtgcatttcg
     6781 acgtgcccgt ccggcaccag ctcggtccac atcgccgcct cgcccccggc gacgaggcgc
     6841 tgcccggcct ccgtcagctc cggcgagtcc gacgccacca tcgcctcggt gacgtaggcc
     6901 tcgagcgggg tccccttcag cgtggccagc atctcggccg ccatgcccgt cgcgcgggtg
     6961 tcgaacgggt ccacctggta gtagcgggca gcgggcagcc cgtgatcaag atagtacccg
     7021 gccgacacga tggtccggtg ccccgccgcg gttgcgcggt gcaccatctt ggagctgcgc
     7081 caggcctgca cgacgacgcc aggcgggagg tcggcatgca acgcctcgtc ccatacggtc
     7141 ggtgtcttgc cgagcgcgac ggcaatggcc gccaccctcc gggtgaaata ggcctgcagc
     7201 tcgtgcgccg attcgaaccc ccggcgccgc atgaacgccc ggatcgcggg attctcgagc
     7261 cactgcgcgc ccgtcacctc gtcgccggca atgtggatcg aggcgtcggg gaacagcccg
     7321 gccagctcgc ccagcagtcc cgcgacgaag cggtacgtct cctcgcgcga cgggtcgagc
     7381 gcggccagca tcgtgtcggg cctccggccg cgtgccatcg gcttcgagct gcttcccagc
     7441 tccgggtacg cgaccaggat gctctgcacg tgccccggca tctcgatttc gggcaccacg
     7501 cggatccccc ggtcgcgggc cgccgcgacg atggcacgga tgtcggcctg cgtgtagaac
     7561 tggccgccgg aggaaccgta ggcggtcagg cgggggtagc gccggctctc cacgcggaag
     7621 ccctcgttgt cggagaggtg caggtgcagc acgttcagct tcaccgactc catcgcgtcg
     7681 agcgtgcgga ggatctcgtc tttcgcgacg aagtggcggg cgacgtcgag catcagtccg
     7741 cgccagctga agcgtggggc gtcctccacg tgcaccgccc gcgcccggat gccgtccgcc
     7801 cccacgcgca ccagctgctg catcgtcgcc agcccgcgga gaacgccgcc ggcggcaggg
     7861 gcctcgagcc gcgcgccgtc cgggccgacg tcgagcgtgt agcgttcgtc ggcgaaggga
     7921 tctgctcccg gtccgctcgc gactccccac cggatccgga gcgtgccggg gcccgagcgc
     7981 agcggcgtca acctgaccgg gaggcccgtg cgcgcctgca ggcgcgtgag aaaccgctcc
     8041 acagccccgg cgacgatggg atccgccggg ccttcgagca cgaccgtgaa gtgctcgtcc
     8101 acggcgagcg acccttcgag ctggaccacg gtcgcaggca ccggcagcag cgccagtgcc
     8161 gccacgccgg gaggcccagc ctgcgcccat gcggacgccg ggagaaagag gacgaagagg
     8221 accaggacgg ccaggcggga aacggcagcg acgagctgtg gcatgagcac tctccgaggc
     8281 cgaagaaggc tatcgtatcg cagcggcagc cttgatcggc tccgtcgcgc tccgcagctc
     8341 gggcagaagc cgcgcccctt gcaggaagag accacgagtc ggtccgtgcc gcacgccgcg
     8401 caccggaaac gcgcgaactc cgccggcagc cagccgcacc gcaggtacgc gcgggactcc
     8461 tcgtctacga accgcggcaa gccctcaccg ccacgcaggc tcgcggcctg cgcgcagcgc
     8521 gatcgctatc cccgggggag ccgggcgaac gatcgccgat gtccagcggc gtcaggacga
     8581 ccgcgcccac acgctcggcg agacagcctt cgtccgtcga agagtccgca agagcgaggc
     8641 gtgagaggaa ccggcggtga cctgcctgat gtgatcggcc ttcgctcgcc cttggcgagc
     8701 tgtggcgaga cagccttcgc atgtcaggga gttcgcgaga gctgggcggg cgaggatttg
     8761 acgctgactt gcctgccgag ccgtagctca cgcgaagcgt gagcgaagat cggcggctat
     8821 tccggatgcg gaatagtggc tgggaggcag ggattcgaac cccgataacg tggttcagag
     8881 ccacgtgtcc taccattgaa cgacctccca gcatggaggg ccgcggcggg accaacctca
     8941 ggattttacc agaacccgca gcgccaatcc acgcgagagc ggcgcgcgcg gcgaggccct
     9001 cgccccggcc cgtgctagca tgggcccgct tcccccagag gcgacaggcc gcatgagcag
     9061 cgccccgccg acaatcgcca tccgcgcggc gaacctgacg cggcgctatg gcgacatcct
     9121 cgccgtcgac gacctctcgc tcgaggtgcg gcgcggcgag atcctcgggc tcctcggccc
     9181 caacggcgcc ggcaagacga cgacgctccg gatgctgtgc gggctgctgc gcccggacag
     9241 cggccgcgtc gagatcgacg gccggcccct gaaggcgggc cggccaggcg gcctgcccgc
     9301 catcggcctg tcgccccagg cgatcgtcat ctgggagctc ctgacggtct tcgagcagct
     9361 ggacttcatg ggccgcgcct atggactccc gcgccgggcg gcccgcgccc gcgccgaagc
     9421 gctgctcacc gcgttcggcc tcgaggagaa gcgcgatcga ctcggccgca cgctctccgg
     9481 cggcctgcag cggcgcctca atatcgcgct cgccttcgcg cacgacccgc ccatcgtcct
     9541 tctcgacgag ccccaggccg gcctcgatcc gcagagccgc gtcctggtgc gcgactacat
     9601 cggctcgctg cgggggcgcg tcacggtcat cgtcacgacg cacgacatgg acgaggcgga
     9661 gagagtcgcg gaccgcgtct gcatcgtcga tcgcgggcgg atcctggcgc tcgacacggt
     9721 cgacgccatc aagggccgcc tcggggaggg cgacgtgatc gaagccgagt tcggcggcgc
     9781 cgacgcggcc gcgctcgacg ccctggccgc cgcgctggcg cgcggcggcc gcccgtgcgc
     9841 gcggcgcgga acgtgcctcc gcttcacgtc ggcggaaggc ttttcggcca tggccgacct
     9901 cctccaggcg gcctcggcgc tgggcctgca ggcccggcgc ctcgccgtgc gcaagcagac
     9961 gctcgaagac gtgttcatcc ggctcaccgg gcgggggctg cgcgaatgag gatgctggcc
    10021 tgcctcagga agacgctcgt cgagaacgtc cgcgactgga agatcctcat cctgacgctg
    10081 agcttcgcgc cgttcttcgt gtacctgatg tacggctacc tgcacaccgc gccggccagc
    10141 tacacgctgc tcgtcctgaa cgacgaccgc gcggcgtcgc cgccgcggga cgccgccgga
    10201 gcggacggcc tgatcgccgc gtggacgcgg gccggcggcg acgacggccg gcccgtgttc
    10261 gccgtgcgcg ccgccggcga tcggcaggcc gcggagcgga tggtgaagac gcgcgaggcc
    10321 gacctgctgg tggtcatccc ggcgggcttc tcggcccgcc tcgccgccct tcgggcgcag
    10381 ccggccggcc cgcccccgcg gctcacgaac caccacaacg ccgccaaccc gcgctcgatg
    10441 atggccatgg ccatgtcgga ctacgtggcg ttcctgtacg cgtcagccgc cgcgagcatg
    10501 ccctcgccgc tcgccgtgga cgccagggcc gtcgatccgc tcccgaaccg gagcgacttc
    10561 gacctctacg tgccggcgct gctcgtgctc gccgtcatca tggtgctctt caccgcggcg
    10621 gcctcgctca tcagggaggt cgacaagggc acgatgaccc gcctgacgct ctcgcgcgtc
    10681 gccacatggg agctgctcgc ggcggtcacg atcacccagg tgctcatcgg cgcggccacg
    10741 gtgctgctgg cgtacgccgc cgcctcctcc gtgggctacc gcgccctggg atcgccgggc
    10801 gccgcgctgg ccgtgaccac gctggctacg gtgagcgtcg tggcgatcgg cgtcctggtc
    10861 tcggccttca tgagaacggt tttcgaattg ctgacggtcg gctgtttccc gttcttcatc
    10921 ctgatgttct tctcggacgt gatgatcccg ctgcccagga tccccgtgct ctccgtggcc
    10981 gggaacgccg tgaacctgag cgacgtgctc ccgacgagcc tcgcggtgcg ggcgctcaac
    11041 cgggtcctga acgacggggc cggcctgggc gacgtgtgct tcgagatgac cgggatggcc
    11101 ctgctcgccg cggcctactt ctccgcggga atccttctgt tccgccggcg gcacctgcga
    11161 acgaattagc cggcggcccg cgcgtgccag ccggcctcca tcatctgcga gagacccggc
    11221 ctacgctccc agcctgaggg cccggccgat gcgcgcgagg acgcgctcgc ggcccagcag
    11281 ctccagcacc tcgaagaggc cgggactctc ggcgcggccg acgacggcca cgcgcgtcgg
    11341 gtgaacgagc gcgcccgcct tcacgccgcg cgacccggca agcgcgcgga tggcgggctc
    11401 gagcgccgcg gcggcgaatg gctccaccga cgcgcacgcc tcgccgacgg ccgccaggtg
    11461 cgcgcgggcg tccggcgacg acaggtgctt ctgcacggcg gccgcgtcgt attcgatgtg
    11521 ctcaacgagg aagggcctcc ccttctcgac gaagtcggtg aggcgcttcg cccgcgggcg
    11581 caggagttcc acgaggcgcg cgagccacgc gagcgcgggg ccgggcgccg aggcgcccgg
    11641 cgattccggg cccggcctga ccggccacag ccccgcttcc tcgagcagcg gctcgacgcg
    11701 gcgcacaagc tccggcgccg gcatccgggc gatgtgctgc gcgctgaacc actcgagctt
    11761 ctcgggattg aagacggcgt tgctgaggct gatgccctcg agcgagaacg ccgcgacgag
    11821 ctcgtcgcgc gtgaacagct cctggtcgct gcccggcgac cagccgagca gcgcgaggaa
    11881 gttcatcatc gcctcgggca ggtagccctg gcgcctgtac tccgtgacgg acgtcgcgcc
    11941 gtggcgcttg ctcagccgcc gcttgtcggg ccccaggatg agcgggacgt gcgcgaaggc
    12001 gggcagcggc ttcccgaacg cgcggtagag cagcacctgc ttcggcgtgt tcgagatgtg
    12061 atcgtcgccg cgcaccacgt gcgtgatcgc catgtcgatg tcgtcggcca ccaccgacag
    12121 gtggtacgtc gggtggccgt ccgagcgcag cgcgacgaag tcctcgatgt gcgcgttgtc
    12181 gaacgtgatg tcgccgtgca cgaggtcgcg aaaggccgtc tcgccttcgg gcacgcggaa
    12241 gcggatcgcg cgcggcgcgc cggccgcctc gcgagccgcc acctcgtcag gcgtcagccg
    12301 cgagcagcgg cggtcgtagg tccacgactc gccgcgggga gtgccgcctt cgccgccctc
    12361 gcgatcggcg gccgagcccc cgccagaggc ggcggccctg gcgtcgccgg cgaaggcggg
    12421 cccatccggg cgctccttgt ccgtgccgca gtagcaatag tacgcgtgcc cctcgcgcac
    12481 gagctgctcg gccagctcgc ggtaccgcgc cagccgctgc gactggaagt acggcccgtg
    12541 cgcgcccccc gcctcgggcc cctcgtccca gtcgagcccg agccaccgca tcccgtcgag
    12601 gatcccggtc accatgtccg ccgacgaccg ctcgacgtcg gtgtcctcga tgcgcagcac
    12661 gaacgcgccg ccggtcttgc gcgcgtagag ccagttgaag agcgccgtgc gcgcgccgcc
    12721 gacgtgcagg tagccggtgg gtgacggggc gaagcggagg cggggggtgg acatgggagt
    12781 agacgctgga caccaggcgc caggggcctg gcgctcggga ctggtcagtt ccgctgcacc
    12841 ctgcactgaa ccgccgagca gcggaagatg tcgcacgcgg ccgcagccgc gccgaaccag
    12901 cgcctggcgc caggttcctg gagcctgaca ggatgcacgt tgtggcggtg aggcagggat
    12961 tcgtccgccg ccgcaccgct ccggccgctc atcttcctcg cggcgcgatg gcgagatttc
    13021 atcccgccgc ccggagccat cgcgccggag ctcgtgccct gtctccaagg cgcgggcgaa
    13081 ggcggatggc ggtgaggcag ggattcgaac cctggataca ggttttggcc cgtataacgg
    13141 tttagcaaac cgccgccttc agcctctcgg ccacctcacc gtccaaacct tcgacgctgc
    13201 cagaatagcc tacttcctgc ccttcccggc cctcttcgca gctttcttca ccggcttcgc
    13261 ggccttcttc accgccctct tgaccggctt gacggccttc ttcgccttct tcgcaacctt
    13321 cttcacggcc ttcttcgccg gcttcgcggc cttcctgaca gctttcttcg ccggcttcgc
    13381 ggccttcttc accgccctct tgaccggctt gacggccttc ttcgccttct tcgcgggctt
    13441 cttcacggcc ttcttcacgg ccttcctggc cttcggcgcg gcttccttca cgttctccgc
    13501 cgccttgacc gcgacggcct tcacggcttc gagcggggac ggcgcggcgg gttcggccgc
    13561 gggcggcgcg gctggcggcg ttgcggcgac aagcggcggg gcttcaggct tcagctcttc
    13621 gggcatggtt tcctccacga cacacatcca tccgcggcgc ggattccggc tcacgcgcag
    13681 tatagaagcg agggttgtca ttgtctaggc cgcttccttc gtcttgccgt caggagacgc
    13741 tcgaagatcg tccgcacggc agtcgggatc gcacggctca ccccgggcgg gatcttctgc
    13801 gatcggcgtg acgccctctg ggtgatcggc ggcgccttta ccgcgcgcgg cctcggctgc
    13861 gttgtctcaa ccggcgcgcg ccgtgccggc tgtcggcgcc gctggggatg ctcgagggcg
    13921 gcgcgtcggc gtgacggcct gcgggcgagt cccgcccggc tggcgccaag ccccgagccg
    13981 ccagccgctg aagtaagatc gtcccgccat gcgtcccctc aagatcggca tcagcggcgt
    14041 ccgcggcatc gtcggcgagt cgctgacgcc ggagctcatc gtctcgttcg cgcaggggtt
    14101 cggcacctac ctcgactcgg gccgcatcct cgtctgccgc gacacccggg cgtcggggcc
    14161 gatggtccac gcggccgtcg tctcgggcct gctggccgcc ggatgcgaag tcgtcgatct
    14221 cggggtgtgc ccgacgccgt cgctgcagca cgcggtgccg tggctcggcg cccagggcgg
    14281 catctccatt tccgccggcc acaatccgtc ggtgtggaac gccctcaagt tcgtgcgcaa
    14341 cgacggcctc tacctgaacg cggcccaggc ggaggaactg ctcgacatcg tccaccaggg
    14401 cgagttcgag aaggcggcct gggatcgcat ccggacaggc gtgcccgtgc aggacgccgt
    14461 cgggcaccac ctcgaggtgc tgtccggcgc gttcaatctc gacgcggcgc tgcgccgcgc
    14521 cctgaaagtc gcggtggact gctgcaacgg cgcctcgacg ttcctgtcgc cccgctggct
    14581 cgaggcgctc gggtgcgagg tgctggccat caacgacgat ccggaggcgc ccttcccgca
    14641 ccggcccgag ccgaagcccg agatgatggc gcagctcaag gcggtggtgc gcgccgggcg
    14701 cgccgacctg ggcttcgcgc acgacgccga cggcgagcgg atcgggctgg tcacgggaga
    14761 cggcgaggcg ctgtccgagg agcgcacgct cacgctggcg gccgccatcg cgctgtccca
    14821 cacgccgggc gtggtcgtca ccaacatctc caccacccgc gccgtggacg atgtggccgg
    14881 gcggttcggg gggagcgtcg tgcgcacgcc cgtgggccag tcgttcatca ccgaggcgat
    14941 gatcgagcac cgcgccgtgc tcggcggcga gggcaacggc gcggtggcgg tgccgcgcgt
    15001 gctgatgtcc cacgacgccg cggcgaccca gggcctggtc gtcgagcaca tggcggtgac
    15061 cggggcctcg ctgcgcgagc tggtggacgc cctgccgtgc tacgagatgg tgaagcgcga
    15121 cttcgcgctg gcgccgggcg cgctctactc caggctgcag gacttccgcg accggatcga
    15181 gcgcgagggg ctgcccgcga acacgatcga cggggccgtg gtgtcgttca ccgacggctg
    15241 ggtgcacgtc cgtgcgtcgc aaacggagtc gatgattcgc gtcatcgcgg aggccgcgtc
    15301 gcgcgaacgg gcgcaggacc tggtggactg ggcgagggac aggctgggga cgggg
//
LOCUS       DAOSIF010000016        15301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4006_length_15301_cov_5.394399, whole genome shotgun sequence.
ACCESSION   DAOSIF010000016 DAOSIF010000000
VERSION     DAOSIF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15301
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4006_length_15301_cov_5.394399"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLT35_01320"
     CDS             complement(<1..254)
                     /locus_tag="PLT35_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27891.1"
                     /translation="MRDCGAASAALAASLLAAVLALTVGSREVAAQESPPGPPPGEPV
                     VLWPEGVPGALPGAGAEAVEEGGLVSNVHQPTLTPYLAPEG"
     gene            complement(911..2428)
                     /locus_tag="PLT35_01325"
     CDS             complement(911..2428)
                     /locus_tag="PLT35_01325"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOG27892.1"
                     /translation="MPPSSLLALSDASLAIVPDSVVAGGGASWGLARTPDGPRLVVAP
                     DAAPGAPPPGFVGARGERAGRAIFVGPVGEANAAVLRRSLSWLVPRPLPPGTSAGFGD
                     RMGVATPGHVRALRAVGAPAVPIAPIFAQQSIREMSRSGRTPQAVIDDATWGAFEAGW
                     TAGVGADADHLKTPADVDACAAAGFSFFTIDPGAFVNDRAEAAPAEELRQAFDALPWD
                     RLESSGVSLRRSLAGRVLDVDGHRVPIDEAAVVKAAVKYGAAVAHVAMMYRHLASVKA
                     GEAFDLEVSVDETDTPTSHAEHVYIASELQRLGVRWISLAPRFVGRFEKGVDYIGDVE
                     AFESDIATHAAIARHFGSYKLSLHSGSDKFSIYEIAARHTRGSVHLKTAGTSYLEALR
                     AVARVAPDLFRAVYAFSRDRYEEDRASYHVSARLDRTPEPAALASGDVAGLLDQFDAR
                     QVLHVTFGSVLTARDAAGAPLFADAVLALLKSHPEVYAECLERHFARHLRPFVRS"
     gene            2648..3439
                     /locus_tag="PLT35_01330"
     CDS             2648..3439
                     /locus_tag="PLT35_01330"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOG27893.1"
                     /translation="MRADGTTPKVNRDSTERVISHVRDLIARGELHPGDRLPAERDLS
                     ARLGVSRPTIRAGLRALAAMGVVKPRHGSGTYIPEGPPTLVTEPLRFLAALHGLTREE
                     MYEARRVLEVSAAGLAAERASAEHVAAIAEEVASLFASIGDPVQFLVHDIRFHRAVAA
                     ACGNPIIASLVEMVAALYYEQRRETAARATDRNLRDAADLHRGIYLAIRKRDADHAKA
                     LMNRHLLHSAAYQAEEERPHPLPAGRRKPARKGRGGRVAPARSRD"
     gene            3456..5522
                     /locus_tag="PLT35_01335"
     CDS             3456..5522
                     /locus_tag="PLT35_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27894.1"
                     /translation="MKRHQFRAAGAGAAGRLAAAAAVFALALPGLRAQAAGQAPADAP
                     GPEAAARISFDMVVIDRDGRAPDRLGPNDVSVSVAGRPRAVTAIRRVSRGPGAAADAA
                     SRLASVPAGGAWTFASEASRAFVVIVDETGIMRGQERQAVTASRLFLDRLGMGDRVAA
                     LVLPLTGSQLLSLTTDQPEVRAALSGVTGRILPSLRGRPEPAITAPEPGSGLRDEPEP
                     PPQPLRTDQPGMPNLDTARLGAGGGLGGLAGVLEAMRSMPGRKTVAFFSAGTADLPLA
                     RVHDVAAAAVAARTAVYAFGLSGGFDASESGLDARPLEALAQATGGRFVALGRNPERA
                     IGRTAAELSSCYVVELEAAADDAGPQRRELKVTLEGRGWTVRAPAWLAPTEDPGDTPA
                     AAAVEPPVDERAAAAPKDERRNPAPPSAGDAARASELQLAMGRLFDYVEAYERQYSGL
                     VAEEDYQQSTRSGDRVRIRSDYLLVKLQGPRGWESFRDVYEVNGVAVRDRDDRLRRLF
                     LEPGSLVEGQLQAIKNESARHNIGLVERNINVPLFTLRFLSPENRDRFHFKLAGRREV
                     DGIGVWRIEYEETGRPTIITDLGNRDVPAKGWFLVDQTTGAIVESRLRAEERGTHGEI
                     LVTFRQDPELGMWVPEKMSETYRSVIPPSGRDLARTGTVQQGTATYSKFRRFVVRTEE
                     TIAIPR"
     gene            5590..6792
                     /locus_tag="PLT35_01340"
     CDS             5590..6792
                     /locus_tag="PLT35_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG27895.1"
                     /translation="MHARHRARMPFVLLAALAAAAAGVGGGPPVLAAPAAARPARAHA
                     QEAQQAQKAQDAPTTRPPRQSPRLQVPMEAERARLLYVSKDPAHQSIGTDYQRDIDAR
                     VASEARYAKACQGVIDFQKVSYRSRIGDLDIPAYLFQPVEKKGPRAHAAMVWVHGGVH
                     GNWGITMFPFVREAVERGYVVIAPDYRGSIGYGEAYHQEIDYGGYEVDDVVSAADFLA
                     SLPHVDPARLGIMGWSHGGYITLLAVFRDDHPFAAGAAIVPVTNLIFRLSLKGPSYQW
                     DFATQKRIQGLPFEKPEIYIERSPLYHVDKLQVPLLVHVSTNDTNVNYVEDQQIVDAL
                     RSRKPDLAETKTYVDPAPWGPSVGHSFSRRVDPDTLQRVDSPDQIDSWNRTWTFFEWN
                     LREKPTKR"
     gene            complement(6944..7912)
                     /locus_tag="PLT35_01345"
     CDS             complement(6944..7912)
                     /locus_tag="PLT35_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG27896.1"
                     /translation="MGDKIPACPNCGPSLTGSGRRCSRCGRELLYLGQEDLVGWPPGA
                     AAAQARSENRAQPRGVWAGVTAAAVAVLALAGVIRPWDWLGRPVAGGQARMTASASER
                     AARQAAAALPEASAPAAAADVGSAEAVRQDELEQTLERYLAALKERPSDAALLDRIGQ
                     TLVRLNRPAAAVPFLERAVESQPGSLVARFDLAGAYAQSGQPAKAADQYGVLVKAGSI
                     DARVHHNHGLALRLLGRNAEALAAFHRATALAPDRAPAWLALALSLDAAGQKPEAVAA
                     LERYLSLQPAGQEAEDARAELIRLRAASPPAAPASETGSAEPIRQP"
     gene            complement(8117..10051)
                     /locus_tag="PLT35_01350"
     CDS             complement(8117..10051)
                     /locus_tag="PLT35_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG27897.1"
                     /translation="MTAVTTLPHLFETSADRFSRNVFLMEKRHAGYEGTTYGDLRARV
                     HRFAAGLMSLGLEKGDRAALIAEGRTDWVVAELGILYCGAINVPISVKLDEPSDLAFR
                     LRHAGCKMAVVSGGQAGKIRAVKAGLPELKLTVVLDQVEAPEPDELAADAVLARGDAF
                     LETGRPALEARWRSIAGSDPANICYTSGTTADPKGIVLTHRNYTANIEQGVAMIQPRE
                     DWVLLNVLPWDHAFGHTCGIYMMMSRGAALASVAAGRTPLETVKNIPAAIRETRPTIF
                     LSVPAIAANFRKNIEKGVREKGAFAAGLFAKALRAAYAYNAEGWNRGAGAQALKKPLL
                     AVYDALVFRAIRANFGGRLRYFIGGGALLDIELQRFFYAIGIPMLQGYGLTEAAPVIS
                     ANTIERHKLGSSGRILPDMEVRICDAGGQPLPPGSRGEIVVRGENVMAGYWRNDAATA
                     EAIRDGWLYTGDLGYLDADGFLYVLGRMKSLLIADDGEKYSPEQIEEAVTERSRFIDQ
                     MMLYNDQSPYTVALLVPARELVLAWLKKQGLSAGTIEGQDAVLRLLDAEIAKYRQGGE
                     FAGMFPGRWLPSAVAVLDEGFTEQNHLLNSTLKMVRGKIVERYRDRIARLYTAAGRPI
                     LNGDNRAAVSRLDPHAGAAG"
     gene            10157..11500
                     /locus_tag="PLT35_01355"
     CDS             10157..11500
                     /locus_tag="PLT35_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOG27898.1"
                     /translation="MHRRSFLWTLASAAAFPGLRASTAWRAGLASIDITPPLGLWMGG
                     FAARTEPARGIALPLHAKALALEDAAGRRAVVVTLDLLGLTDRVAERIAAAVGRRYGV
                     PREGLLLCSSHTHSGPVIDDQLAVAYDLSPSQWEDIRASTRRIEGQVLDVVGRALDAL
                     APAHLRRSQGEAGFGANRRVAFEPPGPVDPAVPVLAVERPGGRLAGLLFGYACHNTTL
                     VASFVQYHGDYAGVAQAELERRYPGAIAMFVQGCGADANPKPRGTRELAEQHGRTLAD
                     AVERALGGGSDVRGPLRRAFGTVTLPYAQAPGAEDWRRKLEDANVYVRRHAKLMLNDI
                     ARDGRTRAAEAVPLHVLALGQSLFIGIGGEVVADYALALKRKYGDATWVAGYVDAVFG
                     YLPSLRVLREGGYEGADAMLYFGRPGPFSEAVEDTVMAGLDGLVARAGALKGRLP"
     gene            11753..13273
                     /locus_tag="PLT35_01360"
     CDS             11753..13273
                     /locus_tag="PLT35_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG27899.1"
                     /translation="MTLVEAFVRTAKTHGDRLAIVDRLTGHRVSYKHALLRSLILARK
                     LEGYDPGFLGVMIPNTAGAILTVIAATMTGRTPVMINYSSGAARNCQDAQRRLGFRTI
                     VTSRALLAKIKCPEVDGMVFLEDIAASVSLFQKVAGSIRATRSVDGVLASLPTLSADD
                     KVVILFTSGSERSPKVVPLTHRNLLANMEGLHGAVVYGEGDVILGNLPYFHVFGFNTG
                     LWLPLTNGMTVVTVPSPLEYRAICAAIRDERVTMLVGTPAFLAGYLQKSEPGDFESTK
                     LIVTGADKCPESLRQGYWERHRRILLEGYGTTETSPVISVNSPDRNRPGSVGRVLPNV
                     EVRMVHYETGDPCPTGAIGRILAKGPSVMSGYFDDFEANATRMQRGWYDTQDMGYMDE
                     DGYLWHVGRLARFLKIGGEMVSLVHVEDVLERAVPPGVVCSVVEVPDAARGARIVAVV
                     SEAVDEREVLRQMGGELPNFALPKQFVVVPEIPKMSSGKIDFRTLTETVRGLVRHA"
     gene            13324..14844
                     /locus_tag="PLT35_01365"
     CDS             13324..14844
                     /locus_tag="PLT35_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG27900.1"
                     /translation="MTLVEAFVRTAKTHGDRLAIVDRLTGHRVSYKHALLRSLILARK
                     LEGYDPGFLGVMIPNTAGAILTVIAATMTGRTPVMINYSSGAARNCQDAQRRLGFRTI
                     VTSRALLAKIKCPEVDGMVFLEDIAASVSLFQKVAGSIRATRSVDGVLASLPTLSADD
                     KVVILFTSGSERSPKVVPLTHRNLLANMEGLHGAVVYGEGDVILGNLPYFHVFGFNTG
                     LWLPLTNGMTVVTVPSPLEYRAICAAIRDERVTMLVGTPAFLAGYLQKSEPGDFESTK
                     LIVTGADKCPESLRQGYWERHRRILLEGYGTTETSPVISVNSPDRNRPGSVGRVLPNV
                     EVRMVHYETGDPCPTGAIGRILAKGPSVMSGYFDDFEANATRMQRGWYDTQDMGYMDE
                     DGYLWHVGRLARFLKIGGEMVSLVHVEDVLERAVPPGVVCSVVEVPDAARGARIVAVV
                     SEAVDEREVLRQMGGELPNFALPKQFVVVPEIPKMSSGKIDFRTLTETVRGLVQRV"
     gene            complement(14874..>15301)
                     /locus_tag="PLT35_01370"
     CDS             complement(14874..>15301)
                     /locus_tag="PLT35_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27901.1"
                     /translation="RDGSEIDRWVTTLTAPDWQASPVSMSTPRLYRARSLPEWRALQS
                     APDPVPTAVREFVQGDRVLVAVDCYGSSPDTHPALSAHVLTREGKELTPLPVPEPAQG
                     AARFELPVGSLGQGTYLVRLRATAGAEQAQELVPIRVAR"
BASE COUNT         2140 a         5578 c         5402 g         2181 t
ORIGIN      
        1 ccctcgggcg cgaggtaagg cgtcagcgtg ggctggtgga cgttgctcac gaggccgcct
       61 tcctccacgg cctccgcacc ggcccccggg agggcgcccg gcacgccttc cggccacagc
      121 acgacaggct cgccaggcgg cgggcccggc ggcgactcct gcgccgcaac ctcacgggat
      181 ccgacggtca gcgccagtac agccgccagc aggcttgcgg ccagcgccgc gctggcagcg
      241 ccacagtccc gcatgccgcc tcctttcgca ccctcccgag cttcgcggcg ccccctcgcg
      301 gtctcccggc gcaaaaagaa accgccgacg cggaggaggg agggtctccg cgccggcggt
      361 cgccagccaa gccgaacggc cagcaggctg ctattctacc gcgttgtcac cgtcacggcg
      421 agaggagacc gattccgccg ggaggcgcgg tccaggcgcc gctcccaatc cgccacggtc
      481 cccggggtca gcgcccctgt tcaaccccga gccggcatga tacgccactt cggccccggc
      541 cggcaggctg atcactgcca gaacgttgcc atcggccccg gagactcccg ccagcctggc
      601 cggatcgacg atgaccgtac agccgaagcg gctgcggtcg gcctcgaccg gttcccacgt
      661 tcggagcacg ccgtcggcct tgtcccgcgc caccggcgcg gcggggtgtc tctcgatgtc
      721 cgcggcctgc gccgtcgagg cgccgcctcc cgctcgccga tgtcgtcctg cgccgccttg
      781 tcgaaggcgc agccggccag gtgcgcgttg cagatcgggg cgtgcacgcg gccgcgcagc
      841 cgcagcccgt gatggctcct gcggctcgcc gcgctccgcg cctcgatgtc gcacgccggc
      901 ggagggccgt tcaggacctg acgaaggggc gaaggtgccg ggcgaagtgc cgctcgaggc
      961 actccgcata gacctccggg tgcgacttca gcagcgcgag gaccgcgtcg gcgaagagcg
     1021 gcgcgccggc cgcgtcccgc gccgtcagca ccgatccgaa cgtcacgtgc agcacctgcc
     1081 gcgcgtcgaa ctggtcgagc agcccggcca cgtcgccgct tgcgagggcc gcgggctcgg
     1141 gcgtccggtc cagcctggcc gacacgtgat agctggcgcg gtcctcctcg tagcggtccc
     1201 gcgagaacgc gtacaccgcg cggaacagat cgggcgccac cctggccacc gcccgcaggg
     1261 cctcgaggta gctggtgccc gcggtcttca ggtgcaccga gccccgggtg tggcgcgcgg
     1321 cgatctcgta gatgctgaac ttgtccgatc ccgagtgcag gctgagcttg tatgacccga
     1381 agtgccgcgc gatcgcggcg tgcgtggcga tgtcggactc gaacgcctcc acgtcgccga
     1441 tgtagtcgac gcccttctcg aagcggccga cgaagcgcgg cgccaggctg atccagcgga
     1501 cgccgaggcg ctggagctcg ctggcgatgt acacgtgctc ggcgtgcgag gttggcgtgt
     1561 cggtctcgtc caccgacacc tcgagatcga acgcctcgcc cgccttcacc gaggcgaggt
     1621 gccggtacat catcgcgacg tgcgccacgg cggcgccgta cttcaccgcc gccttgacga
     1681 cggccgcctc gtcgatcggc acgcggtggc cgtcgacgtc caggacgcgg cccgcgaggg
     1741 acctgcgcag cgagacgccg ctcgattcca gccggtccca tggcagcgcg tcgaacgcct
     1801 ggcgcagctc ctcggccggg gcggcttcgg cgcggtcgtt gacgaaggct ccggggtcga
     1861 tggtgaagaa cgagaagccc gcggcggcgc acgcgtcgac gtcggccggc gtcttcaggt
     1921 ggtccgcgtc ggctccgacg ccggcggtcc atcccgcctc gaaggctccc cacgtcgcat
     1981 cgtcgatgac ggcctgaggc gtgcggcccg agcggctcat ctcgcggatg gactgctggg
     2041 cgaagatggg agcgatgggc acggcgggcg cgccgacggc gcgcagcgcc cggacgtgcc
     2101 cgggcgtggc cacgcccatg cgatcgccga accctgcaga ggtgccgggc ggaagcgggc
     2161 gaggcacaag ccacgacagc gagcgccgga gcacggcggc gttggcctcg ccgacggggc
     2221 cgacgaagat cgcgcgtccg gcgcgctccc cgcgcgcgcc gacgaagccg ggcggcgggg
     2281 cccccggcgc cgcgtcgggg gccacgacga gccttggccc gtccggcgtc cgggccagcc
     2341 cccagctggc tccgccgccg gcgacgaccg agtcgggcac gatcgcgagg ctggcgtccg
     2401 acagggcgag caggctggat ggcggcatgg gcgtcctcgg cttgggggcc cttgaggtct
     2461 gaccactttt gcgctctcga ttgaatacca ccgggaacgg cttccgtcaa gcgcgggacc
     2521 ccagcacgga tcgagaaagg tcgggccatt ccgcctcgga gccgcgctga aaagtggatt
     2581 gtccactcgg ggatcccagc gcgcctgtca gcccgaagcg gagtatactg gcgcttcgag
     2641 gtggagcatg cgggccgacg gaaccacgcc gaaggtcaac agggacagca ccgaacgtgt
     2701 catctcgcac gtccgggacc tgatcgcccg gggcgagctg caccccggcg atcgcctgcc
     2761 ggcggagaga gatctgtcgg cgagactggg cgtgagccgc cccaccatcc gtgccgggct
     2821 gcgcgcgctc gccgcgatgg gcgtcgtgaa gccgcgccac gggtccggca cctacatccc
     2881 cgaggggccg ccgaccctgg tcaccgagcc gctgcggttc ctcgcggcgc tgcacggcct
     2941 cacgcgcgag gagatgtacg aggcgcggcg agtcctcgag gtgagcgcgg ccggcctggc
     3001 cgccgagcgc gccagcgccg agcacgtcgc cgccatcgcc gaggaagtcg ccagcctgtt
     3061 cgcctccatc ggagaccccg tccagttcct cgtccacgac atccgcttcc accgcgccgt
     3121 ggctgcggcg tgcggcaacc cgatcatcgc gtcgctcgtc gagatggtgg cggcgctcta
     3181 ctacgagcag cggcgcgaga cggccgccag ggccaccgac cgcaacctcc gcgacgcggc
     3241 ggatcttcac cggggcatct acctcgccat tcgcaagcgc gacgcggacc atgccaaggc
     3301 gctcatgaac cggcacctgc tccactcggc agcctatcag gccgaggagg agcgccccca
     3361 tccgcttcct gccggccgtc gaaagcccgc tcggaagggt cgcggcgggc gggtcgcacc
     3421 ggccaggtcg cgtgactgag accaggggca cgcgtatgaa gaggcaccag ttccgcgcgg
     3481 caggcgcagg cgcggcagga cgccttgcag cggccgcggc ggtgttcgcg ctggcgctac
     3541 cgggccttcg ggcccaggcc gcgggccagg cgccagcgga cgcgccgggg ccggaggcag
     3601 cggcccggat ctcgttcgac atggtcgtga tcgatcgcga cgggcgcgcg cccgaccgtc
     3661 tcggcccgaa cgacgtctca gtgtcggtcg ccggcaggcc ccgcgccgtc accgccatcc
     3721 ggcgcgtgag ccggggcccg ggcgcggcgg ccgatgccgc ttcccgcctg gcgagcgtcc
     3781 cggccggcgg cgcgtggacg ttcgcctccg aggcgagccg cgccttcgtc gtcatcgtcg
     3841 acgagaccgg gatcatgcgg ggccaggagc gtcaggccgt taccgccagc cggctgttcc
     3901 tcgatcgcct gggcatgggg gaccgcgttg cggcgctcgt cttgccgctg acaggaagcc
     3961 agctgctgtc gctgacgacg gatcagccgg aggtcagggc cgccctgtcg ggcgtcaccg
     4021 ggcggatcct gccttcgctg cgcggccggc cggaacccgc gatcacggcg cccgagccgg
     4081 gctcgggtct gcgcgacgaa cctgagcccc cgccgcaacc gcttcggacg gaccagccag
     4141 ggatgccgaa cctcgacacg gcccggcttg gcgcaggcgg cggcctcgga ggcctcgcgg
     4201 gcgttctcga ggcgatgcgg tccatgccgg gacgcaagac cgtggcgttc ttttcggcgg
     4261 gcacggccga tctcccgctc gcgcgggtcc acgacgtggc cgcagccgcc gtcgcggcgc
     4321 gcacggcggt gtacgccttc ggcctgagcg gcggcttcga cgcgtcggag tccggcctgg
     4381 acgcgcggcc gctggaggcg ctggcccagg cgacgggcgg gcggttcgtc gcgctcggaa
     4441 ggaaccccga gcgggccatc ggccgcaccg ccgccgagct gtcgtcgtgc tatgtggtgg
     4501 agctcgaggc cgcggccgac gatgccggtc cgcagcggcg cgagctgaag gtgacgctgg
     4561 agggccgcgg ctggaccgtg cgcgccccgg catggctcgc gccgacggag gaccccggcg
     4621 acacccccgc cgcggccgcg gtcgagccgc ccgtcgacga gcgggccgca gcggctccta
     4681 aagacgagcg gcggaacccg gccccgccgt cggcaggcga cgccgcgcgc gcctccgagc
     4741 tgcagcttgc gatgggccgg ttgttcgact acgtggaggc gtacgagcgc cagtattccg
     4801 ggctcgtggc ggaagaggac taccagcagt ccacgcgctc gggcgatcgg gtcaggatcc
     4861 gctccgacta cctcctggtg aaactgcagg gcccgcgggg gtgggagtcg ttccgtgacg
     4921 tctacgaggt gaacggcgtc gccgtccggg accgcgacga ccgcctcagg cgcctcttcc
     4981 tcgagccggg ctccctcgtc gagggccagc tccaggccat caagaacgag agcgcgcggc
     5041 acaacatcgg cctggtcgag cggaacatca acgtcccgct gttcacgctg cgcttcctca
     5101 gccccgagaa ccgcgaccga ttccacttca agctggccgg ccggcgcgag gttgacggaa
     5161 tcggcgtctg gcgcatcgag tacgaagaga ccggtcggcc gacgatcatc acggacctcg
     5221 ggaaccgcga cgtgccggcc aagggctggt tcctcgtgga ccagacaacc ggcgccatcg
     5281 tcgaatcgcg cctgagggcc gaggagcgcg ggacgcacgg cgagatactc gtgaccttcc
     5341 ggcaagatcc cgagctcggc atgtgggtgc cggagaagat gtcagagacc taccggtccg
     5401 tgatcccgcc ctcgggccgg gacctggcga ggaccgggac cgtgcagcag gggaccgcca
     5461 cctacagcaa gttccgccgg ttcgtcgtca ggaccgaaga gacgatcgcg atccccagat
     5521 agccgctgcg cccgggcgga agcgctctgg tatagtgggc ggccggcacc gcccttcctg
     5581 gaggcttcca tgcacgctcg gcaccgcgcc cgaatgccct tcgtcctgct ggccgctctc
     5641 gccgccgcag cggccggcgt cggcggcggc ccgcccgtgc tggcagcgcc cgccgcggcc
     5701 cgaccggccc gcgcgcacgc ccaagaggcc cagcaggccc agaaggccca ggacgctcca
     5761 acgacgcgtc cgccgcgcca gagcccgcgc ctgcaggtgc cgatggaggc ggagcgcgcc
     5821 cggctgctct acgtgtcgaa ggacccggcc caccagtcga tcggtaccga ctaccagcgc
     5881 gacatcgacg cgcgcgtggc gtcggaggcg cggtacgcga aagcctgcca gggcgtgatc
     5941 gacttccaga aggtgtccta ccgcagccgc atcggcgatc tggacattcc ggcgtacctg
     6001 tttcagcctg tcgagaagaa gggccccagg gcgcacgcgg cgatggtctg ggtgcacggc
     6061 ggcgtccacg ggaactgggg catcaccatg ttccccttcg tccgcgaggc ggtggagcgc
     6121 ggctacgtcg tcatcgcgcc cgattaccgc ggcagcatcg gctacggcga ggcctaccac
     6181 caggagatcg actacggcgg ctacgaggtg gacgacgtcg tgtcggcggc ggacttcctg
     6241 gcgtcgctgc cgcacgtgga ccccgcgcgc ctcggcatca tgggctggag ccacggcggg
     6301 tacatcaccc tgctggccgt gttccgggac gatcatccct tcgcggcggg cgcggccatc
     6361 gtgcccgtga cgaacctcat cttccgcctg tcgctcaagg gcccgtccta ccagtgggac
     6421 ttcgccacgc agaagcggat ccaggggctg cccttcgaaa agccggagat ctacatcgag
     6481 cggtcgccgc tgtaccacgt ggacaagctg caggtgccgc tgctggtgca cgtttccacc
     6541 aacgacacca acgtgaacta cgtggaggac cagcagatcg tggacgcgct gcgctcgcgg
     6601 aagcccgatc tggcggagac gaagacctac gtcgacccgg cgccgtgggg gccgagcgtc
     6661 gggcactcct tcagccgccg cgtcgatccg gacacgctcc agcgcgtgga ctcgcccgat
     6721 cagatcgact cgtggaaccg gacgtggacg ttcttcgagt ggaacctccg cgagaagccc
     6781 acgaaacgat gacgggttcc gatggccggc aaggcgccgg cgctgcctgt tcgagtgccg
     6841 gccgggggcc gatcgggaca tgcgcgcagg cggctgccgg gccggacggg tccgttgcgg
     6901 ccgagacgat tccgtgcggg gatccgggcc ctgggcccgg cgctcatggc tgccggatcg
     6961 gctcggcgct tcccgtctcg gaagccggcg ctgcaggcgg tgatgccgcc cgcaggcgga
     7021 tgagttcggc ccgagcgtct tcggcctcct gccccgcagg ctgcagcgag aggtagcgtt
     7081 cgagcgcggc gacggcttcg ggcttctgtc ccgcggcgtc caggctgagc gcgagcgcca
     7141 gccacgccgg agcccggtcc ggcgccagcg ccgtggcgcg atggaacgcc gcgagggcct
     7201 ccgcgttcct gccgagcagg cgcagcgcga ggccgtggtt gtgatgcacg cgcgcgtcga
     7261 tcgaccccgc cttgacgagc acgccgtact ggtccgcggc cttcgccggc tggccgcttt
     7321 gcgcgtaggc gccggccagg tcgaagcgcg cgaccaggct gcccggctgc gactccacgg
     7381 cccgctcgag gaacggtacg gcggccgccg gccggttgag gcgcacgagg gtctgcccga
     7441 tgcggtcgag cagcgcagcg tcggacggcc gctccttcaa cgcggccaga taccgttcca
     7501 gggtctgctc cagctcgtcc tggcgaacgg cctccgcgct cccgacgtcg gctgcggcgg
     7561 ccggcgcgga cgcttccggc agcgcggcag cggcctgtct ggctgcccgc tcgctcgcgc
     7621 tcgccgtcat gcgcgcctgg cctccggcaa ccggcctgcc cagccaatcc cagggacgaa
     7681 tgacgccggc cagcgcgagc acggcgacgg ccgccgccgt cacgcccgcc cacaccccgc
     7741 gcggctgcgc ccggttttcg ctccgggcct gcgcggcggc ggcgccaggc ggccagccca
     7801 cgaggtcctc ctggccgagg tagagcagtt cgcggccgca gcggctgcat cgccgtcccg
     7861 agccggtgag gcttggcccg cagttggggc acgcgggaat cttgtcgccc atggatcgcc
     7921 gcgtggccgc ctgctgccgc atgcccgatc tttcggcgcg gcaacagccc ggctccagcc
     7981 ctcctgcacg gcaattcccg cgccagcacg gctcgtctcg cgtcgggccc gccggacggc
     8041 cttcgaacga gcgcggcccg gtgcggcgcc gcggcggagc gtgccccgga accggacagg
     8101 cgtggccgga aagacgtcag cccgccgcac ccgcgtgcgg gtcgaggcgc gacacggccg
     8161 cgcggttgtc gccgttcagg atgggccgcc cggccgccgt gtacaggcgc gcgatgcgat
     8221 cgcggtagcg ctcgacgatc ttgccccgga ccatcttcag cgtggagttg aggaggtggt
     8281 tctgctcggt gaacccttca tccaggacgg ccaccgccga cggcagccag cggcctggga
     8341 acatcccggc gaattcgccg ccctggcggt acttcgcgat ctcggcgtcc agcagccgca
     8401 gcaccgcgtc ctgcccctcg atggtcccgg ccgacagccc ctgcttcttc agccatgcca
     8461 ggacgagttc ccgcgccgga acgagaaggg ccacggtgta gggcgactgg tcgttgtaga
     8521 gcatcatctg atcgatgaat cgggagcgct ccgtgacggc ctcctcgatt tgctccgggc
     8581 tgtacttctc gccgtcgtcg gcgatcagca ggctcttcat ccggccgagc acgtagagga
     8641 acccgtcggc gtcgaggtag ccgaggtccc cggtgtagag ccagccgtcc cggatcgcct
     8701 cggcggtcgc ggcgtcgttc cgccagtagc cggccatcac gttctcgccg cggacgacga
     8761 tctcgccgcg cgatcctggc gggagcggct gtccgccggc gtcgcagatg cgcacctcca
     8821 tgtccggcag gatccggccg gaggatccca gcttgtgccg ctcgatggtg ttggccgaga
     8881 tgaccggcgc ggcctcggtc aggccgtagc cctgcagcat cgggatcccg atggcgtaga
     8941 agaagcgctg cagctcgatg tcgagcagcg cgccgccgcc gatgaagtac cgcagccgtc
     9001 cgccgaagtt cgcgcggata gcgcggaaga cgagcgcgtc gtagacggcc agcagcggct
     9061 tcttcagcgc ctgggcgccg gcgccgcggt tccagccctc cgcgttgtag gcgtacgccg
     9121 cgcgaagggc cttcgcaaac aagccggcag cgaacgcgcc tttctcgcgc acgcccttct
     9181 cgatgttctt ccggaagttc gcggcgatgg ccggcacgct gaggaagatc gtcggccgcg
     9241 tctcgcggat cgcggccggg atgttcttca ccgtctccag cggcgtccgg ccggcggcca
     9301 ccgacgccag cgcggctccc cggctcatca tcatgtagat gccgcacgta tggccgaagg
     9361 cgtggtccca cggcaggacg ttcagcagca cccagtcctc gcggggctgg atcatggcga
     9421 cgccctgttc gatgttcgcg gtgtagttcc ggtgcgtcag gacgatgccc ttggggtcgg
     9481 ccgtcgtgcc ggaggtgtag cagatgttgg cgggatcgct gccggcgatg gatcgccagc
     9541 gcgcttcgag cgccggtctc ccggtctcga ggaaggcatc gccccgggcg agaacggcgt
     9601 ccgccgccag ctcatccggc tcgggcgcct ccacctggtc gagcacgacc gtcagcttca
     9661 gctcaggcag gccggccttg acggccctga tcttgcccgc ctggccgccg gacacgacgg
     9721 ccatcttgca gccggcgtgg cggagccgga acgcgaggtc ggacggctcg tccagcttga
     9781 ccgagatggg cacgttgatg gcgccgcagt agaggatccc gagctcggcc accacccagt
     9841 cggtgcggcc ctcggcgatg agggcggccc gatcgccctt ctcgaggccg aggctcatca
     9901 gccccgctgc gaaccggtgc acgcgagcgc gcagatcgcc gtacgtcgtg ccctcgtagc
     9961 ccgcgtgccg cttctccatg aggaacacgt tgcgcgagaa ccggtcggcg ctcgtctcga
    10021 acaggtgcgg cagggtcgtc acggcggtca tgcgggcgct ccgaggcggg ggccggggga
    10081 agcttatccg atcggagccc ggccttgcat gggtgccgat ggaggcgcat cgggccgatg
    10141 gaggtatcat cccgccatgc accgccgctc cttcctctgg acgctggcgt cggctgcggc
    10201 gtttccgggc cttcgggctt cgacggcctg gcgcgccggg ctggcgtcga tcgacatcac
    10261 gccgccgctc ggcctctgga tgggcgggtt cgcggcccgg actgagcctg cccgcggcat
    10321 tgccctgccg ctccacgcga aggccctggc cctggaggac gcggccggcc gccgcgcggt
    10381 cgtcgtcacg ctggacctgc tcggcctcac tgatcgggtg gccgagcgca tcgccgccgc
    10441 cgtcggccgc cgctacggcg tgccgcgcga ggggctgctg ctctgcagtt cgcacacgca
    10501 cagcggcccg gtcatcgacg accagctggc cgtcgcctac gatctcagcc catcccagtg
    10561 ggaggacatc cgcgcctcga cgaggcggat cgagggccag gttctcgacg tcgtcggccg
    10621 cgccctcgac gccctcgcgc ccgcccatct ccggcggtcc cagggcgagg ccggcttcgg
    10681 ggccaatcgg cgagtggcct tcgaaccgcc gggcccggtc gatcccgccg tgccggttct
    10741 cgcggtcgaa cgtccaggcg gccgcctggc cggactgctg ttcggctacg cctgccacaa
    10801 cacgacgctg gtggcgtctt tcgtccagta ccacggcgac tacgccggcg tggcgcaggc
    10861 cgagctggag cgccgctacc cgggtgcgat cgccatgttc gtgcagggct gcggcgccga
    10921 cgcgaacccc aagccccgcg gaacccggga actcgccgaa cagcacggac ggaccctggc
    10981 cgacgccgtc gagcgggcgc tcggcggcgg gtccgacgtc cggggaccgc tccgccgggc
    11041 cttcggcacg gtcacgctgc cctatgccca ggcgcccggc gcggaggact ggcgccgcaa
    11101 gctcgaggac gcgaacgtct acgtccgccg gcacgcgaag ctgatgctga acgacattgc
    11161 ccgcgacggc cggacccgcg cggccgaggc cgtcccgctc cacgtcctgg cgcttggcca
    11221 gtctctcttc atcggcatcg gcggcgaagt cgtcgccgat tacgcgctgg ccctgaagcg
    11281 caagtacggc gatgccacct gggtggccgg gtacgtggat gcggtcttcg gctacctgcc
    11341 gtccctgcgc gtgctgcgcg agggcggcta cgaaggcgcg gacgcgatgc tctacttcgg
    11401 gcgccccggc ccgttctccg aggccgtcga ggacacggtc atggcgggcc ttgacggcct
    11461 cgtggcgcgc gcgggcgcct tgaaaggccg ccttccttag cggtcgaggc cttcgccttc
    11521 gcttcgtctt tggatgcgtc tttttgatcg ccctcggacg ttgtgtcgta tactcagccc
    11581 tcgccgtgcg cccgctcgga gggccgcgtc ggcttcgtgt cggcttcgcc agacacgcgg
    11641 gcggcgccat cccgttcggt gcctcagggt ttcgggccgg gagtcacgac gccggcctcc
    11701 gcacgcccgg acaccgcccg ggagccgcaa cgccccgtag ctggacacag tcatgactct
    11761 tgtcgaagcg tttgtccgga cggccaagac gcacggcgac cggctcgcga tcgtcgaccg
    11821 cctgaccggg catcgcgtga gctacaagca cgccctgctc cggtcgctca tcctcgcgcg
    11881 caagctggag ggctacgacc ccggcttcct cggcgtcatg atccccaaca ccgccggggc
    11941 gattctcacc gtcatcgccg cgaccatgac cggccgcacc ccggtgatga tcaactactc
    12001 cagcggcgcc gccagaaact gccaggacgc ccagcgccgc ctcgggttcc gcacgattgt
    12061 cacgtcccgg gcgctcctgg cgaagatcaa gtgccccgag gtggacggca tggtcttcct
    12121 cgaggacatc gccgcctcgg tctccctgtt ccagaaggtg gccggctcca tccgggcgac
    12181 ccgctcggtc gacggcgtgc tggcctccct gccgacgctg tccgccgacg acaaggtcgt
    12241 catcctgttc acgagcggca gcgagcggtc gccgaaggtc gtgcctctca cccaccgcaa
    12301 cctccttgcg aacatggagg gcctccatgg tgcggttgtc tacggcgagg gcgacgtcat
    12361 cctcggcaac ctgccgtact tccacgtctt cggcttcaat accgggctgt ggctccccct
    12421 caccaacggc atgacggtgg tgacggtccc gagcccgctc gagtaccgcg ccatctgcgc
    12481 ggccatccgg gacgagcgcg tgacgatgct cgtcggcacc ccggcgttcc tggcgggcta
    12541 tctccagaag tccgagccgg gcgacttcga gagcaccaag ctcatcgtga ccggcgcgga
    12601 caagtgcccc gagtcgctcc ggcagggata ctgggagcgc caccggagga tcctgctcga
    12661 ggggtacggg acgacggaga ccagccctgt catctcggtc aactcgcccg accgcaaccg
    12721 cccgggcagc gtcggccgcg tgcttccgaa cgtcgaggtg cgcatggtgc actacgagac
    12781 gggcgacccg tgcccgacgg gcgcgatcgg ccggattctg gcgaagggcc cgtcggtcat
    12841 gtcgggctac ttcgacgact tcgaggcgaa cgccacccgc atgcagcgcg gctggtacga
    12901 cacccaggac atgggctaca tggacgagga cggctacctc tggcacgtcg gccgcctcgc
    12961 ccgcttcctt aagatcggcg gcgagatggt ctccctcgtg cacgtcgagg acgtgctcga
    13021 gcgcgcggtg ccgccgggcg tcgtctgctc cgtcgtcgag gtgcccgacg ccgcgcgcgg
    13081 ggcccggatc gtcgccgtcg tcagcgaagc cgtcgacgag cgcgaggtgc tccgccagat
    13141 gggcggcgag ctcccgaact tcgcgctgcc gaagcagttc gtcgtcgtgc ccgagatccc
    13201 gaagatgtcg agcggaaaga tcgacttccg gacgctcacc gagaccgtgc gcggcctcgt
    13261 gcgccacgcc tgacccccgg aggccggaag ccggaagccg agatctccca tgaggacgca
    13321 gtcatgaccc ttgtcgaagc gtttgtccgg acggccaaga cgcacggcga ccggctcgcg
    13381 atcgtcgacc gcctgaccgg gcatcgcgtg agctacaagc acgccctgct ccggtcgctc
    13441 atcctcgcgc gcaagctgga gggctacgac cccggcttcc tcggcgtcat gatccccaac
    13501 accgccgggg cgattctcac cgtcatcgcc gcgaccatga ccggccgcac cccggtgatg
    13561 atcaactact ccagcggcgc cgccagaaac tgccaggacg cccagcgccg cctcgggttc
    13621 cgcacgattg tcacgtcccg ggcgctcctg gcgaagatca agtgccccga ggtggacggc
    13681 atggtcttcc tcgaggacat cgccgcctcg gtctccctgt tccagaaggt ggccggctcc
    13741 atccgggcga cccgctcggt cgacggcgtg ctggcctccc tgccgacgct gtccgccgac
    13801 gacaaggtcg tcatcctgtt cacgagcggc agcgagcggt cgccgaaggt cgtgcctctc
    13861 acccaccgca acctccttgc gaacatggag ggcctccatg gtgcggttgt ctacggcgag
    13921 ggcgacgtca tcctcggcaa cctgccgtac ttccacgtct tcggcttcaa taccgggctg
    13981 tggctccccc tcaccaacgg catgacggtg gtgacggtcc cgagcccgct cgagtaccgc
    14041 gccatctgcg cggccatccg ggacgagcgc gtgacgatgc tcgtcggcac cccggcgttc
    14101 ctggcgggct atctccagaa gtccgagccg ggcgacttcg agagcaccaa gctcatcgtg
    14161 accggcgcgg acaagtgccc cgagtcgctc cggcagggat actgggagcg ccaccggagg
    14221 atcctgctcg aggggtacgg gacgacggag accagccctg tcatctcggt caactcgccc
    14281 gaccgcaacc gcccgggcag cgtcggccgc gtgcttccga acgtcgaggt gcgcatggtg
    14341 cactacgaga cgggcgaccc gtgcccgacg ggcgcgatcg gccggattct ggcgaagggc
    14401 ccgtcggtca tgtcgggcta cttcgacgac ttcgaggcga acgccacccg catgcagcgc
    14461 ggctggtacg acacccagga catgggctac atggacgagg acggctacct ctggcacgtc
    14521 ggccgcctcg cccgcttcct taagatcggc ggcgagatgg tctccctcgt gcacgtcgag
    14581 gacgtgctcg agcgcgcggt gccgccgggc gtcgtctgct ccgtcgtcga ggtgcccgac
    14641 gccgcgcgcg gggcccggat cgtcgccgtc gtcagcgaag ccgtcgacga gcgcgaggtg
    14701 ctccgccaga tgggcggcga gctcccgaac ttcgcgctgc cgaagcagtt cgtcgtcgtg
    14761 cccgagatcc cgaagatgtc gagcggaaag atcgacttcc ggacgctcac cgagaccgtg
    14821 cgcggcctcg tgcagcgggt ctgacgggcg cctggccgcg cgcgccgcgc ggctcagcgc
    14881 gccacgcgga tcggcaccag ttcctgggct tgttccgcgc ctgccgttgc ccgcaggcgc
    14941 acgaggtagg tgccctgtcc gaggctgccg acgggcagct cgaacctggc cgcgccttgc
    15001 gccggctcgg gcacgggaag cggcgtgagc tccttgcctt cacgcgtcag cacgtgggcg
    15061 ctgagggcgg ggtgcgtgtc ggggctcgaa ccgtagcagt ccacggcgac caggacgcga
    15121 tcgccctgca cgaactcgcg cacggcggtg ggcaccgggt ccggcgccga ctgcagcgcc
    15181 cgccactcgg gcagcgagcg cgcgcgatag aggcgcgggg tcgacatcga cacaggcgac
    15241 gcctgccagt ccggcgcggt cagcgtcgtc acccaccgat cgatttccga cccgtccctg
    15301 g
//
LOCUS       DAOSIF010000017        15083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4082_length_15083_cov_4.739553, whole genome shotgun sequence.
ACCESSION   DAOSIF010000017 DAOSIF010000000
VERSION     DAOSIF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15083
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4082_length_15083_cov_4.739553"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLT35_01375"
     CDS             complement(<1..558)
                     /locus_tag="PLT35_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG27902.1"
                     /translation="MRSVLFVVVGVALAADAAAQSPDGPMTLTLDAALRIAEARNPGV
                     VVAQQAAAAAEADLAGASRRPNPSLSLESEGLLSRRSTPFADGQQIQLALQQELETGG
                     RRGLRTEQARLALDSSRASAQDALRQLRFDVRLAYLRVVLAKADEEVTRETLAEIDRV
                     LAINRARYDAGELAGVELRRLQVERF"
     gene            complement(614..952)
                     /locus_tag="PLT35_01380"
     CDS             complement(614..952)
                     /locus_tag="PLT35_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27903.1"
                     /translation="MTRLRRFTCAPAIAVLLLAAAWSDIVLHGFVELDSAPIGLSGPS
                     PALAAGSGAIGAQRTPPDHCFCHSVSTGARSLPLAAVFELLGSAWPPLTPSAVRADRR
                     PADQPPRPVA"
     gene            1268..3502
                     /locus_tag="PLT35_01385"
     CDS             1268..3502
                     /locus_tag="PLT35_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HOG27904.1"
                     /translation="MENARTRAVLLFILVLAFGVLLFGGHLMNREKPPIPAEVVTPAG
                     QPVYSGADVIGGQNYFYSRGGQHMGSIWGHGAYLAPDWSADYLHRMGLFVAARHLGKT
                     PEEAAAFTQADLERLDAATRARVTALAIEEQKTNRYDAASGRLTFTDFQADAHASLVR
                     YYTELFERGDSRMGLQANIVRSPEEGRLVASFFSWLAWAAGTNRPGESHTYTSNWPFD
                     PLVGNRPMPDALIWSIVSVILLILGIAAAIFLYQKYVREDDYAASPVTTLGEPAPTPS
                     QKATLAYFFIAIVLFLLQGALGSLTAHFTVEGTSFLGVPIGGILPYAAVRTWHLQLGI
                     FFIATCFLAAGLFIGPYVGREPKHQGTLVWVLLAAVVVVVLGALGGTYASIQGAFGGD
                     GFMLGHQGYEYIELGRLWQVLLIAGMIIWLVLVVRAIRPALTSEKDAGGITHLLLYSA
                     IAIPAFYMVGLLYGKGSHLSNAEYWRWWVVHLWVEGFFEVFATVFMAFLLARIGVVGP
                     RFALRTVYFSIFLYLGSGVIGTFHHLYRTGAATPIIALGAVFSALEVVPLTLVGFEAV
                     HNLRAAGGAGSNPYRWPIYFFVSVAFWNMLGAGVFGFLINPPIVLYYIQGINTTPIHS
                     HTALFGVYGLLAISLMLFSMRHTVSRAGWSDTLLKWSFWGLNGGLALMTFVSLVPSGF
                     YQFYYAVRDGMWYARSPEIASGEVIRALALARVVPDLVFLAGAALLLVFVGRAIWPGA
                     RRTA"
     gene            3537..3887
                     /locus_tag="PLT35_01390"
     CDS             3537..3887
                     /locus_tag="PLT35_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG27905.1"
                     /translation="MSGTHDRKAAEPFGGGKALDLVSYQAGAVVSRQIIKKKTGSVTA
                     FAFDQGEGLSEHTAPFDALVHVLDGSADITIGGVTHLVGAGELILMPANVPHAVKAEQ
                     PFKMLLVMIREPPA"
     gene            4563..5228
                     /locus_tag="PLT35_01395"
     CDS             4563..5228
                     /locus_tag="PLT35_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG27906.1"
                     /translation="MRSDTMSPRQAQLVEAAMRIIATKGSRRFTAQLLADEVGVTPGA
                     IYRHFPSLNAIVDAVVDRVGALLFADVPPDARDPIPRLGAFFRCRCRAILANPALSRL
                     LLSDHLAQAAGGAQAKRLEGFRRRSRDFVAGCLREAAARGQLAAGIEPDAGAVIVLGS
                     ILALCHSGTRIVDAEGFERLAEAVWAALERALRRRAPAVRRPMAAPARVGRGGSTVRG
                     GTR"
     gene            5225..5782
                     /locus_tag="PLT35_01400"
     CDS             5225..5782
                     /locus_tag="PLT35_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOG27907.1"
                     /translation="MKATQELVTEHDAVLLALRILEKVEAAVAANREEAPEHLGQLVE
                     FFRVFVDHCHHGKEEEALFPELERRGIPRDGGPIGVMLMEHGLGRERVAGMAAGLDEL
                     RKGVPGAVAAIRAHAAAYRDILRSHIHKENSVLFPMADRVVPADAADALAAKFEDIER
                     DRIGAGRHEAFHAMLSALKGYYGTA"
     gene            6007..7365
                     /locus_tag="PLT35_01405"
     CDS             6007..7365
                     /locus_tag="PLT35_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG27908.1"
                     /translation="MNGPEPLRPRAARIGFWMTTALVIGNTIGSGIFLLPAALAPFGA
                     DNVPGWIATAAGGLTLALVFARLGLLVPGAGGPYAYTRDAFGDLAGFTVGWAYWVSIW
                     VGNAAIATAAVGYLGSLVPWMTASPLNTALATLGVVWVFTLVNCAGIRAAGWAQAVTT
                     LLKLVPLVAAIGLLLTRLDEVRWPGGSGAPFTLGGTVSVVTLALWALLGLESATIPAD
                     KIEGAGKTVSRATVWGTALSAAFSAAACAAVLLLLPAARVASSSAPFADLASAAWGAR
                     AARAVSLFAAVSALGALNGWILLQGELPRAMADDGLFPRAFARTSRNGAPAAALLATS
                     AMVTALVLLTVSRTLVGIFTFFALLATTATLAAYLACALALLRVRARRRSDGARAGLA
                     GAGIVACLGAAYSVGALVGAGREAVLWGAVLLLAGLPIHWWLQTRGPGARRLPTAGVG
                     SA"
     gene            7527..8033
                     /locus_tag="PLT35_01410"
     CDS             7527..8033
                     /locus_tag="PLT35_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG27909.1"
                     /translation="MRPRVAPSPDLSSSLSACIARRGLKSTRQRQAILGAFLASSGHV
                     TVVDLLARARAADPGVSPATVYRTLRLLAECGLAKPQRFHGSPTMYEIVAGREHHDHL
                     VCTRCGRVVEFEDARIERLQTAVARARGFTVTSHRMELYGLCRLCRPARPGRKAGSAG
                     RRSRAKRR"
     gene            8017..8676
                     /locus_tag="PLT35_01415"
     CDS             8017..8676
                     /locus_tag="PLT35_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOG27910.1"
                     /translation="MRRDAKAAFLEVVLGLTRRGAPARTRDIAENLGIRPSAAGKMAV
                     DLAHEKLVARAPGGVVLTEAGRAIALTVAERHRRLEAFLAEDLGMDARQAHREARAAE
                     QDLPAEALDLVCTHLERTARSGAGPAAGDGPSASEERAGAVRALSDLDEDEAGTIRVV
                     VAGRDTRHDLMSLGFLPGVEVRVRRKLRADSLLVRIKEAEIAIGRNVAKGILLAAAGH
                     A"
     gene            8673..10559
                     /gene="feoB"
                     /locus_tag="PLT35_01420"
     CDS             8673..10559
                     /gene="feoB"
                     /locus_tag="PLT35_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOG27911.1"
                     /translation="MKGDGVRAVVLVGNPSVGKSTLFSQLTGVGVLISNYPGATVEVA
                     RGRVRHDEFALELFDLPGVYSLDTDGPEERAVRRCLADGPPDVVLNVVDASRLERNLY
                     LTLELLELGLPVVVALHMNEDATRRGMSIDVERLAVLLGVPVVLVSARSGQGLEELVH
                     ALGAPPDPGRNRQVGYPRCVEAALRKAQSLGLSRRRAMTLLASGAATDETPEVLAAIA
                     AARAEAECRRGASIAELAAGSRYGDAGLIAQEVTRRRPARRLRRERFDDILMHPFAGL
                     ALVGATLLAMLLVVFFLGGFLEEWIVGGLDRLVLVPVRTALEGLGLLEVVVEYTLIGV
                     QAGLGIVVPYIMTFYVLMALMENSGYLTRAAFLLDAPMHRLGLHGRSMIPLILGFGCS
                     VPALMATKSLQTRRERVVTSALVSLVPCSARTIVILGLVAHFVSVWAALSIYLLMLVL
                     IVIAGFILGRTVKGERTGFVLEMVPLRAPAVREVAEKTWMQMKEFVFLAFPLLIAGSA
                     MLGALQHLGILGAVNAALSPITSGWLGLPEYSATALLFGVLRKEMALETLAVMAGTAD
                     FGSVLTPLQMYVFAVFTSIYVPCLASMAVLKRVLGWRDMAWITAFTVGLAFLLSGLIN
                     QALV"
     gene            10601..11653
                     /locus_tag="PLT35_01425"
     CDS             10601..11653
                     /locus_tag="PLT35_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOG27912.1"
                     /translation="MEGRAGWLAAGLASAVWMVGAAAPAGAQAAGPVARAEGRASSPL
                     RKVVVASVLTRFTGGLDERLATAARLADRAAAEAARTAGGRGLDLLVFPESAIQRPGR
                     TAAEQAVPLEGAVLTTLGSKAREHRTWLVVPMTLSEGGAAGPISNAAVLLDRAGRVAG
                     IYRKVRPMIDESGVAEGGVRPGSQYPVFDADFGRLGILICWDQNYEEAWDALAAARAE
                     IVALPSASPQTIRPAAQAMRHALYVMTSTPRDNASLVDPIGRVSARITTPGVLVREID
                     LAFAILHWSERLRDGRALAERFGTKAGFSYSSREDTGVFWSNDPHRSIGSMIRELGLR
                     QMVDEIERVRHGTARK"
     gene            complement(11747..13318)
                     /locus_tag="PLT35_01430"
     CDS             complement(11747..13318)
                     /locus_tag="PLT35_01430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOG27913.1"
                     /translation="MNAAAVARSIAPDAALSPPDEGRLVVPVALGDWEAAARALHADH
                     RLPLDLLWATDDRAASGSFGLHAAFALDASHEWIELVTAVPGADPVFPSITPRVMAAH
                     WYERAVQDMFGLTAAGHPDPRRLVHHENVPPGTWPLRKDFPWDAKLPWAAEPHPLATV
                     EGEGVFQVPVGPIHAGVIEPGHFRFSVAGERIITLEGKLFFTHKGVEKLLEGRTAAAA
                     MPFVERVSGDSAASHALAFCQAVEAVARCRVPERARVLRVLAVELERFTMHLHDVANI
                     CGMGTGFTLLAAGGFRVKERLQRLSARLFGNRFFRGFVLPGGTARDLGAPELDDIEAT
                     LDGAWAEASGLFQIGLESDSLLDRLETTGVLSRDAAWAYGAKGIAARASGLDRDSRRD
                     HPHAAYGTLPLRVPVRTEGDVDARVRIRLDEMAQARALVARGAAECRRSQGAPWSAPC
                     EPADGDALGWTEGWRGQILDWVRFRGGVIDRAVVRDPSFCNWPLFGELGPGNIVPDFP
                     LCNKSLNLSYSGTDL"
     gene            complement(13315..14808)
                     /locus_tag="PLT35_01435"
     CDS             complement(13315..14808)
                     /locus_tag="PLT35_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG27914.1"
                     /translation="MSLLLLLLVPAAAAVLAAAAPDARSAGRGAVAASLAGAAALAAA
                     AWPVVAAGERWFGGHWMLDRFTVTMAGLVVMVWVSAAAASTRYVGAEHDAGILPLRKV
                     RLYYVLMPVFVAAMLVTLLTDHLGVLWIALEVTTLATTPLVALYRKDGAVEAAWKYLL
                     LCSVGISVSLLGLLLLAYAGVRGGLPLGSALSLSALRGAAGSLDPQVVRWAFVLLFVG
                     IGTKVGFVPLHPWLPDAHSRTPSPVSAMLSGVLLNVALYALLRSRTIVDLALGSAAWT
                     SGFFLAFGLASVLFAAFVLLHQQNYKRMLAYHSVEHMGLIAFGLAMGPAGAAGAVMHM
                     VGHTLAKSALFLSAGEILLRVHSTKIPNVGALRGRMPRTSIAFLLGFLGLIGVPTSAI
                     FASELTFLMAAARLAPAAALAIVLALVIVAVGVLHHVFEMIYGRVDAGGDRPGATPGS
                     PVREAWTLAHVVVGAQLALLFAGGVFFLTPGGYAAAERVAAAFTVRP"
     gene            complement(14805..>15083)
                     /locus_tag="PLT35_01440"
     CDS             complement(14805..>15083)
                     /locus_tag="PLT35_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27915.1"
                     /translation="SGALGWFLVFFLLGFLLIIIRRDLYSQIIGFLTLENGISAFAVV
                     ALGGVPFLMEMGIFAVAASGVVLMAVLSGQVHALYASHDTDALRELTD"
BASE COUNT         2053 a         5378 c         5540 g         2112 t
ORIGIN      
        1 gaaccgctcg acctgcagtc tgcgaagctc gaccccggcc agctcgccgg cgtcgtaccg
       61 cgcacggttg atggccagca cccggtcgat ctccgcgagt gtctcccgcg tgacctcctc
      121 gtcggccttg gcgagcacca cccgcaggta ggcgagccgg acgtcgaagc ggagctggcg
      181 aagcgcgtcc tgcgccgacg cccgcgaaga gtcgagggcg agccgcgcct gctcggtccg
      241 cagcccccgc cggcccccgg tctccagttc ctgctggagc gcgagctgga tctgctggcc
      301 gtccgcaaac ggcgtgctcc gccgggagag caggccctcc gattccaacg agagagaggg
      361 attgggccgg cgactggcgc cggccagatc cgcctcggcc gctgcggcgg cctgctgcgc
      421 gacgaccacg cccgggttgc gcgcctcggc gatgcggagc gcggcgtcga gcgtaagcgt
      481 catcgggccg tcgggcgact gggcggcggc atcggcggca agcgccacgc cgacgactac
      541 gaacaggaca cttcgcatgg cagtctcacg atcgcgaacg ggagttggcg tgaagagaac
      601 gggagcgggc ggactacgcg acgggcctgg gaggctggtc ggcggggcgg cgatcggcgc
      661 ggacggcgga cggggtgaga ggcggccagg cgctcccgag aagctcgaag acggccgcca
      721 gaggcagcga gcgcgctccc gtcgaaacgc tgtgacagaa gcagtggtcg ggcggggtcc
      781 gctgcgcgcc gatggcgccg gagcccgcgg cgagcgcggg cgaggggccc gacaggccga
      841 tcggcgcgct gtcgagttcg acgaacccgt gaagcacgat gtcgctccag gccgccgcca
      901 gcagcagcac cgcaatcgcg ggagcgcacg tgaaacgccg caaccgggtc atggtcccaa
      961 gccagcatac cacggcgccg gccgcgggtc gccaggcgcg cgtccgggcg cgggcccgcc
     1021 gcccgggagg gggcccgccg gccaatccgc gccctgcgtc gcgtgagtct ccggcgcccg
     1081 acagcggtcg cagcgcgcgc ccgatcggcg ggggccgggc cgggcggccc gagggagggc
     1141 aggatgcgcc cggcgggcgc cgcttgtaag taagcgatta ctcaccatat agttgatggt
     1201 cgcgggttcc cgcacgtcgc cgcgcgcgtg gcagcagacg cccagtcggg aaccggaggg
     1261 ttgagtcgtg gagaacgcga ggacacgagc ggttctgctg ttcatcctgg tcctggcctt
     1321 cggagtgctc ctgttcggag gccatctcat gaaccgcgag aagcccccga tcccagccga
     1381 ggtcgtcacg cccgccggcc agccggtgta ctccggggcc gacgtcatcg gcggccagaa
     1441 ctacttctac agccgcggcg gccagcacat gggcagcatc tgggggcacg gcgcctacct
     1501 cgctcccgac tggtcggccg actacctgca ccggatgggc ctgttcgttg ccgcgcggca
     1561 cctggggaag acccccgaag aggccgccgc cttcacgcag gccgacctcg agcgcctcga
     1621 cgcggcgacc cgcgcccgcg tgacggcgct ggcgattgag gaacagaaga ccaaccggta
     1681 cgacgcggcg tccggccggc tgacgttcac cgacttccag gccgacgccc acgcgagcct
     1741 ggtccgctac tacaccgagc tcttcgagcg gggcgactcg cgcatgggcc ttcaggcgaa
     1801 catcgtccgc agcccggagg aaggccgcct cgtcgccagc ttcttctcgt ggctcgcctg
     1861 ggccgccggc accaaccgcc cgggcgagtc ccacacctac acgagcaact ggcccttcga
     1921 tccgctcgtc ggcaaccggc cgatgccgga cgccctcatc tggtcgatcg tcagcgtcat
     1981 cctgctcatc ctcggcatcg cggcggccat cttcctctac cagaagtacg tcagggaaga
     2041 cgactacgcc gcctcgcccg tgacgacgct cggcgagccc gcgccgacgc cgagccagaa
     2101 ggccacgctc gcgtacttct tcatcgcgat cgtgctgttc ctgctgcagg gcgccctggg
     2161 ctccctgacg gcgcacttca cggtcgaggg cacgagcttc ctcggcgtgc ccatcggcgg
     2221 catcctgccc tacgccgcag tgcgcacctg gcacctccag ctcggcattt tcttcatcgc
     2281 gacgtgcttc ctcgccgcgg ggttgttcat cggtccgtac gtcgggcgcg agcccaagca
     2341 ccagggcacg ctggtctggg tgctcctcgc cgccgtcgtc gtggtggtgc tcggcgcgct
     2401 cggcggcacg tacgcgtcga ttcagggcgc gttcggcggc gacggcttca tgctgggcca
     2461 ccagggctac gagtacatcg agctcggccg cctctggcag gtgctgctca tcgccggcat
     2521 gatcatctgg ctcgtgctgg tcgtgcgcgc catccggccg gcgctcacca gcgagaagga
     2581 cgccggcggc atcacgcacc tgctccttta cagcgccatt gccatcccgg ccttctacat
     2641 ggtggggctg ctgtacggga agggcagcca cctctcgaac gccgagtact ggcggtggtg
     2701 ggtcgtccac ctgtgggtcg aaggcttctt cgaggtcttc gccacggtgt tcatggcgtt
     2761 cctgctggcg cgcatcgggg tcgtgggccc ccgcttcgcg ctgcgcaccg tgtacttcag
     2821 catcttcctc tacctcggca gcggcgtcat cggcacgttc catcacctgt accggaccgg
     2881 cgcggccacg ccgatcatcg ccctcggggc ggtgttctcg gcgctcgagg tcgtgccgct
     2941 gacgctcgtc ggcttcgaag cggtccacaa cctgcgcgcg gccgggggag ccggatccaa
     3001 tccgtaccgc tggccgatct acttcttcgt gtcggtcgcc ttctggaaca tgctcggggc
     3061 tggcgtgttc gggttcctca tcaatccgcc gatcgtgctc tactacatcc agggcatcaa
     3121 caccacgccg atccactccc acaccgcgct cttcggggtg tacggtctgc tggccatctc
     3181 cctgatgctc ttctcgatga ggcacaccgt cagccgggcg ggatggtccg acacgctgct
     3241 gaagtggagc ttctggggcc tgaacggcgg cctcgcgctc atgaccttcg tgagcctcgt
     3301 gccgtccggc ttctaccagt tctactacgc ggttcgcgac ggcatgtggt acgcgcgcag
     3361 cccggaaatc gcctccggcg aggtcatccg ggcgctcgcc ctggcgcgcg tcgtgccgga
     3421 cctggtcttc ctggccggcg cggccctgct cctggtcttc gtgggacggg cgatctggcc
     3481 cggcgcgcga cgcacggcgt gagcgcggcg ccgcgcgagg cgaagggaga acccggatga
     3541 gcggaacgca cgacaggaag gccgccgagc cgttcggcgg cggcaaggcg ctcgacctag
     3601 tgtcgtacca ggccggcgcg gtggtgagcc gtcagatcat caagaagaag accgggtcgg
     3661 tgacggcctt cgccttcgat cagggcgaag gcctcagcga gcacacggcg ccgttcgacg
     3721 cgctcgtgca cgtgctggac ggctccgccg acatcaccat cggcggcgtg acgcacctgg
     3781 tcggggcggg cgagctgatc ctgatgcccg ccaacgtgcc gcatgccgtc aaggctgagc
     3841 agccgttcaa gatgctgctc gtgatgatcc gcgagcctcc cgcgtgagga tcgccgcggg
     3901 gccggcgcgc ttcgggcggc cggccccggg ccggccgctg cggaactgcc gcgcccgggc
     3961 gacgcccggc gcgcgcgggg ccgggcatcc gacgtcctcc gcctcgcgcg gcatcgtgag
     4021 cctccccgcc agcgtgacga ggccatcgtt cgcgcatgcc gaaacccggc cgaccaccgc
     4081 gcagcgcgcg tcgcggcgcg cttcgcccgg cacgccggcg cggggatcgg aggccctctt
     4141 cggcgccgct ccggccgcca gacgcggaac gggcgctgcg gcctcggcga cgataggcgg
     4201 ctccttcagc gtggttcgca tctcctgcgg cttgcctccg gcgactccgg ccattcgcgt
     4261 cggctggcgt cagccgggca ggacgtcgat cgtcgtccga atccaggacg ggccagctat
     4321 gccgggacaa cacgcgcggc gcgcccggac gagggcggca gccggaggca gggggccgga
     4381 cccctgcgag cgcaaccgcc gcggcacgcc ggcccggcgg accggcacac gaaggccgag
     4441 cggcgccggc ggggacgtcg cctgctggtc gacggagccg gacctggcgg ccggtgccgg
     4501 cattgtgagg caaacactca tcgacataca ctgagccggg agccggtccg gcagggtcgc
     4561 ccatgaggtc agacaccatg tccccgcgcc aggcgcagct ggtggaggcc gcgatgcgga
     4621 tcatcgccac gaagggttcg cgccggttca ccgcccagct cctggccgat gaggtcggcg
     4681 tcacgcctgg cgcgatctat cggcacttcc ccagcctgaa cgccatcgtg gacgccgtcg
     4741 tcgatcgggt cggcgccctg ctgtttgctg acgttccgcc cgacgcgcgg gatccgattc
     4801 cgcgcctcgg tgcgttcttc aggtgccggt gccgggcgat cctcgccaac cccgccctgt
     4861 cgcgcctgct gctctcggat catctcgccc aggccgcagg cggagctcag gcgaagcggc
     4921 tcgaggggtt caggcggcgg tcgcgggact tcgtcgccgg gtgccttcgg gaggccgccg
     4981 cgcggggcca gctggcggcc ggcatcgaac cggatgccgg cgccgtgatc gtgctgggat
     5041 cgattctggc gctgtgccac tccggcaccc ggatcgtcga cgcggaggga ttcgagcgac
     5101 tggcggaggc ggtgtgggcg gccctcgagc gcgcgctgcg gcgtcgggcc ccggccgtcc
     5161 gaaggccgat ggcggcgccg gcccgtgtgg gccgcggcgg ctcaaccgtg aggggaggca
     5221 ccagatgaag gcgacgcagg aactcgtcac cgaacacgac gcggtgctgc tcgcgttgcg
     5281 gatcctcgag aaggtcgagg ccgcggtggc cgcgaaccgg gaagaggccc cggagcacct
     5341 gggccagctc gtcgagttct tcagagtgtt cgtcgatcac tgccaccacg gcaaagagga
     5401 ggaggcgctc ttccccgagc tggagcggcg cgggatcccg cgcgacggcg ggccgatcgg
     5461 cgtcatgctc atggagcacg gcctcggccg cgagcgcgtg gcggggatgg ccgcgggcct
     5521 cgacgagctg aggaagggcg tgccgggcgc cgtggccgcc atccgcgcgc acgcggccgc
     5581 ctaccgcgac atcctgcgtt cgcacatcca caaggagaac agcgtgctct tccccatggc
     5641 ggaccgcgtc gtcccggccg acgcggcgga cgcgctggcc gcgaagttcg aggacatcga
     5701 acgggaccgc atcggcgccg ggcgccacga ggcgttccac gcgatgctga gcgcgctgaa
     5761 gggctactac gggacggcgt gaccgccgcg cgcgcgccgg gagcgtcccg gccgtcgttc
     5821 cctggcctgg cgccggccgg ccgccatgct ggcgtcccga tcggtccggc gcgccgggtc
     5881 cggcacgccg cggcgtcacc gggcttcccc cgcggtgggc ggtcgccgga gtgtcgatga
     5941 gttgcgccga cgcggggcct tcatcgcctg attgcccgcc gtgccgcatg cggttcgagg
     6001 agaaccgtga acggccctga gccgctgcgt ccccgtgccg cgcgcatcgg gttctggatg
     6061 accaccgccc tggtcatcgg gaacacgatc gggtcgggga tcttcctgct gccggccgcc
     6121 ctagccccgt tcggcgcaga caacgtgccc ggctggatcg cgacggcggc ggggggcctc
     6181 acgctggcgc tggtgttcgc gcggctcggg ctgctcgtgc cgggcgcagg agggccgtac
     6241 gcgtacacgc gcgacgcgtt cggcgacctg gccggattca cagtcgggtg ggcgtactgg
     6301 gtctcgatct gggtcggcaa tgccgccatc gccacggcgg ccgtcggcta tcttggctcg
     6361 ctcgtgccgt ggatgacggc ttcacctctc aacaccgcgc tcgcgacgct cggcgttgtc
     6421 tgggtgttca cgctcgtcaa ctgcgcgggc atccgggcag cgggatgggc gcaggcggtc
     6481 acgacgctgc tgaagctcgt gccgctcgtg gccgccatcg ggctgctgct gactcggctc
     6541 gacgaggtgc ggtggcccgg cggttccggc gcgccgttca ccctgggcgg gacggtgtcg
     6601 gtcgtcacgc tcgcgctgtg ggcgctgctt ggcctcgagt cggcgaccat tcccgcggac
     6661 aaaatcgaag gagccggcaa gaccgtctcc cgcgccaccg tgtggggcac ggcgctgagc
     6721 gcggccttca gcgcggcggc gtgcgcggcc gtgctgctgc tcctgccggc cgcgcgcgtg
     6781 gcgtcgtcga gcgcgccgtt tgctgatctg gcttccgccg cctggggcgc gcgagcggcc
     6841 cgggccgtgt ccctgttcgc cgccgtcagc gccctcggcg ccctgaacgg ctggatcctg
     6901 ctgcagggag agctgccccg ggcgatggcc gacgacggcc tgttcccgcg cgccttcgcc
     6961 cgcacctcgc gcaacggcgc gccggcggcg gcgctgctcg caacgagcgc catggtcacc
     7021 gctctggtgc tgctcaccgt gtcgcgcacg ctggtgggca tcttcacgtt cttcgcgctg
     7081 ctggcgacga cggcgacgct tgccgcctac ctcgcctgcg ccctggcgct gctgcgcgtg
     7141 cgggcgcgcc gccggtcgga cggcgcgcgg gccgggctgg cgggcgcggg cattgtcgcc
     7201 tgcctgggcg ccgcgtactc cgttggcgcg ctcgtcggtg cgggccgcga ggccgtgctc
     7261 tggggcgccg tcctcctgct ggccggcctg ccgattcact ggtggctgca gacgcgcggg
     7321 ccgggcgccc gccggctgcc gaccgcgggt gtcgggtccg cgtgaatcag ccgcgcgcgg
     7381 acgcttcgcg cggaagcgcg cagcgccggc cggcgacccg acggcgggcc ccccgacgct
     7441 ccccggccgc cgccggcgct tgacttccgg ccgggacccc gggtacaagc gtagttgaga
     7501 gtgcgtctca aaatccggat caagtcgtgc gtccgcgcgt agccccgtcc cccgacctct
     7561 cctcgtccct cagcgcctgc atcgccaggc gcgggctcaa gagcacccgc cagcgtcagg
     7621 cgatcctcgg cgcgttcctc gcctcgagcg gccacgtcac cgtcgtcgat ctccttgcca
     7681 gggcgcgggc cgccgatccc ggggtgtctc cggcgacggt ctatcggacc ctccgcctgc
     7741 tggcggaatg cgggctggcc aagccgcagc ggtttcacgg ctcgccgacg atgtacgaga
     7801 tcgtcgccgg gcgggagcac cacgatcacc tcgtgtgcac gcgctgcggc cgggtcgtgg
     7861 agttcgagga cgcccgcatc gagaggctgc agaccgcggt cgcgcgggcg cgggggttca
     7921 cggtcaccag ccaccggatg gagctctacg ggctgtgccg tctctgccgg ccggcgcggc
     7981 ccggtcgcaa ggccggcagc gcagggaggc gatcgcgtgc gaagagacgc taaggcggcc
     8041 ttcctggaag tcgtgctcgg cctcacccgt cgcggcgcgc cggcccgcac gcgcgacatc
     8101 gccgagaacc tgggcattcg cccgtcggcc gccgggaaga tggcggtgga cctggcgcac
     8161 gagaagctcg tggcccgggc gccgggcggc gtcgtgctca ccgaggccgg gcgggccatt
     8221 gcgctcaccg tcgccgagcg ccaccgccgg ctcgaagcgt tccttgccga ggacctcggc
     8281 atggatgcaa ggcaggcgca ccgcgaggcg cgggccgcgg aacaggacct gccggccgag
     8341 gccttggatc tcgtctgcac gcacctggag cgcacggcga gaagcggcgc cggcccagcc
     8401 gcgggggacg ggccgtccgc gtccgaggag cgcgccggtg cggtgcgggc gctctccgat
     8461 ctggacgagg acgaggccgg gaccatccgc gtggtcgtcg ctgggcgcga cacgcgccac
     8521 gatctcatgt cgctggggtt cctgcccggc gtcgaggtgc gggtccgccg gaagctccgc
     8581 gcagacagcc tgctcgtgcg aatcaaggag gcggaaatcg ccatcggcag gaacgtcgcc
     8641 aagggcatcc tgctggctgc ggcggggcac gcatgaaggg cgacggagtc cgcgccgtcg
     8701 tgctcgtggg caaccccagc gtggggaaga gcacgctctt ctcgcagctc accggcgtcg
     8761 gcgtcctcat ctccaactac ccgggcgcca ccgtcgaggt cgcccgcggc cgcgtccgcc
     8821 acgacgagtt cgcgctggaa ctcttcgatc tgcccggcgt ctactcgctc gacaccgacg
     8881 gccccgagga acgggccgtg cgccggtgcc tggcggacgg gccgcccgac gtcgtcctca
     8941 acgtggtgga cgcctcccgg ctggaacgca acctgtacct aacgctggag ctgctcgagc
     9001 tgggcctgcc ggtcgtcgtc gcgctccaca tgaacgagga cgcgacacgc cgcggcatga
     9061 gcatcgacgt cgagcgtctg gccgtgctgc tgggcgttcc ggtcgtcctc gtgtccgcgc
     9121 ggtcgggaca ggggctcgag gagctggtgc acgcgctggg cgcaccgccc gacccgggga
     9181 ggaaccggca ggtgggttac ccccgctgcg tcgaggccgc gctgcggaag gcccagagcc
     9241 tcggcctgtc gaggcgacgc gcgatgacgc tgctggccag cggcgcggcc acggacgaaa
     9301 cgccggaggt gctggccgcc atcgcggcgg cccgggcgga ggccgagtgc cggcgcggcg
     9361 cctcgattgc cgaactggcg gccggcagcc gctacggcga cgccgggctg atcgcgcagg
     9421 aggtgacccg gaggaggccg gccaggcggc tgcgacgcga gcggttcgac gacattctca
     9481 tgcacccctt cgcggggctg gcgcttgtcg gggccacgct gctcgccatg ctgctcgtcg
     9541 tcttcttcct cggcgggttc ctcgaagagt ggatcgtcgg cggactggac cgcctggtgc
     9601 tcgtgccggt gcggacggcg ctggagggcc tgggcctcct ggaggtggtc gtcgagtaca
     9661 cgctcatcgg cgtccaggcc gggctgggca tcgtcgtgcc gtacatcatg acgttctacg
     9721 tcctgatggc cctcatggag aactcgggtt acctgacgcg ggccgccttc ctgctcgacg
     9781 ctccgatgca tcgcctcggg ctgcacggcc ggtcgatgat ccccctcatc ctcgggttcg
     9841 gctgcagcgt tccggcgctg atggcgacca agtcgctgca gacgcgtcgc gagcgcgtgg
     9901 tcacgtcggc cctggtcagc ctcgtgccgt gctccgcccg cacgatcgtc atcctcggcc
     9961 tcgtggcgca cttcgtgtcg gtgtgggcgg ccctgtcgat ctacctgctg atgctcgtgc
    10021 tgatcgtgat agccgggttc atcctggggc ggacggtgaa gggcgagcgg accggcttcg
    10081 tgctggagat ggtgccgctg cgcgcccccg cggtccggga ggtcgccgag aagacctgga
    10141 tgcagatgaa ggaattcgtc ttcctggcct tccccctcct catcgccggg agcgcgatgc
    10201 tcggcgcgct ccagcacctg ggcatcctcg gggccgtgaa cgccgcgctg tcgccgatca
    10261 ccagcggctg gctggggctg ccggagtatt cggcgacggc gctgctcttc ggcgtcctgc
    10321 gcaaggagat ggcgctggag acgctcgccg tcatggcggg caccgccgat ttcgggagcg
    10381 tgctgactcc gctccagatg tacgtgtttg cggtcttcac gtccatctac gtgccgtgcc
    10441 tcgcctcgat ggccgtgctc aagcgcgttc tcggctggcg ggacatggcc tggatcacgg
    10501 ccttcacggt cgggctggcg ttcctgctga gcgggctcat caaccaggcg ctcgtgtagc
    10561 cggcggcgcg gcgctggccg ccggccgcga agggggagcc gtggagggac gcgcgggctg
    10621 gctggcggca ggcctggcgt cggcggtgtg gatggtcggg gcggcggcgc cggcgggcgc
    10681 gcaggcggcg ggcccggtgg cccgcgcgga gggccgggca agctccccgc tgcgcaaggt
    10741 ggtggtcgcg tcggtgctga cccggttcac gggaggcctc gacgagcgcc tcgcgacggc
    10801 cgcgcggctc gcggaccggg cggcggccga agccgcgcgg acggccggcg ggcgtggcct
    10861 cgacctgctg gtgttccccg agtccgccat ccagcgtccc ggtcgaaccg cggccgagca
    10921 ggccgtgccg ctcgagggtg ccgtcctgac gacgctgggt tcgaaggccc gcgagcaccg
    10981 cacgtggctg gtcgtgccga tgaccctgag cgagggggga gcggccgggc cgatcagcaa
    11041 cgcggcggtg ctgctggatc gcgcgggccg cgtcgccggc atctatcgca aggtccgccc
    11101 gatgattgac gaaagcggcg tggccgaggg gggcgtcagg cccggcagcc agtacccggt
    11161 cttcgacgcc gacttcggcc gcctcggcat cctcatctgt tgggaccaga actacgagga
    11221 ggcctgggac gcgctcgcgg cggcccgcgc cgagatcgtg gcgctgccga gcgcatcgcc
    11281 gcagacgatc cggccggccg cgcaggcgat gcgccacgcg ctctacgtga tgaccagcac
    11341 gccgcgcgac aacgcgtcgc ttgtcgaccc catcggcagg gtttccgccc ggatcacgac
    11401 gccaggcgtg ctcgtgcggg agatcgacct ggccttcgcg attctccact ggagcgagcg
    11461 cctgcgcgac gggcgtgcgc tcgccgaacg cttcgggacg aaggcgggct tctcgtattc
    11521 gagccgcgag gacacagggg tcttctggtc gaacgacccc catcggagca tcggctcgat
    11581 gatccgcgaa ctcggcctgc gccagatggt cgacgagatc gagcgcgtgc ggcacggcac
    11641 cgcccggaag tgacgcggca ggcccccgtt ttcccgcgag gcgcgcgtac aggccggcgc
    11701 ggccgctgcg ggctccggcc gggagccccg cccggcgtcg gcacggctac aggtcggtgc
    11761 cagagtagct gaggttgagc gacttgttgc agagcgggaa gtccgggacg atgttgcccg
    11821 ggccgagttc gccgaagagc ggccagttgc agaacgaggg gtcgcgcacg acggcgcgat
    11881 cgatgacccc gccgcggaag cggacccagt cgaggatctg gccgcgccat ccttccgtcc
    11941 agccgagcgc atcgccgtcc gccggctcgc agggcgccga ccacggggcg ccctggctgc
    12001 ggcggcactc ggcggcgccc cgcgccacga gcgcccgcgc ctgcgccatt tcgtccaggc
    12061 ggatgcgcac ccgggcgtcg acgtcgccct cggtccgcac gggcacccgg agcggcagcg
    12121 ttccgtacgc ggcgtgcgga tgatcgcgcc gcgagtcccg gtccaggccg gacgcgcgcg
    12181 ccgcgatacc cttcgcgccg tacgcccacg cggcgtcgcg ggacaggacg cccgtcgttt
    12241 cgaggcgatc gagcagcgag tccgactcga gcccgatctg aaagaggccc gaagcctcgg
    12301 cccaggcgcc gtcgagggtc gcctcgatgt cgtccagttc cggcgcgccc aagtcccggg
    12361 ccgtgccgcc cggcagcacg aatcccctga agaaccggtt gccgaacagc cgcgccgaca
    12421 gtcgctgcag ccgctccttg acgcggaacc cgccggccgc cagcagcgtg aagccggtcc
    12481 ccatgccgca gatgttcgcg acgtcgtgga ggtgcatcgt gaaccgctcg agttccaccg
    12541 ccagcacgcg aaggacgcgc gcccgctcgg gcacccggca gcgggcgacg gcttcgacgg
    12601 cctggcagaa cgcgagcgcg tgcgaggccg ccgagtcgcc ggacacccgc tcgacaaacg
    12661 gcatggccgc cgccgccgtg cggccttcga gcagcttctc gacgcctttg tgggtgaaga
    12721 acagcttgcc ctcgagggtg atgatacgct ccccggccac gctgaagcgg aaatggccgg
    12781 gctcgatgac gccggcatga atgggcccca cggggacctg gaacacgccc tcgccctcga
    12841 ccgtggccag cgggtgcggt tcggcggccc aggggagctt cgcgtcccag gggaagtcct
    12901 tgcgcagcgg ccacgtgccc ggcgggacgt tctcatggtg cacgaggcgc cttggatcgg
    12961 gatgccccgc ggccgtgagg ccgaacatgt cctggacggc ccgctcgtac cagtgggccg
    13021 ccattaccct cggggtgatc gacgggaaca ccgggtcggc gccgggcacg gcggtcacca
    13081 gttcaatcca ctcgtgcgac gcatccagcg cgaatgcggc gtggaggccg aaggaaccgg
    13141 aggcggcgcg gtcgtcggtg gcccacagca ggtcgagcgg gaggcgatga tcggcgtgca
    13201 gcgcgcgcgc cgcggcctcc cagtcgccga gcgcgaccgg aacgaccagc cggccttcgt
    13261 ccggcggcga cagggccgca tccggggcga tcgagcgggc aacggcagcc gcgttcatgg
    13321 ccgcaccgtg aaggccgcgg cgacgcgttc tgcggccgca tagccgcccg gcgtgaggaa
    13381 gaagacgccg ccggcgaaga gcagcgccaa ctgggcgccc acgaccacgt gggccaacgt
    13441 ccacgcctcg cgaaccggcg agcccggcgt ggcgccgggg cggtcgcccc cggcgtccac
    13501 gcgcccgtag atcatctcga agacgtggtg gagcacgccg accgcgacaa tcaccagcgc
    13561 gaggacgatg gcgagggcgg ccgccggcgc cagacgggcg gcggccatga ggaacgtcag
    13621 ctcgctggcg aagatcgccg acgtcggcac gccgatcagg ccgaggaacc cgaggagaaa
    13681 cgcgatcgag gtcctcggca tccggcctcg cagcgcgccg acgttgggga tcttggtgga
    13741 gtggacgcgc agcaggatct cgccggcgga gaggaagagg gcgctcttgg ccagcgtgtg
    13801 tcccaccatg tgcatgacgg cgccggcggc gccggcgggg cccatggcga ggccgaacgc
    13861 gatgagcccc atgtgctcga cgctgtggta ggcgagcatc cgcttgtagt tctgctggtg
    13921 gagcagcacg aacgccgcaa acagcaccga cgcgagcccg aacgccagga agaaccccga
    13981 ggtccacgcc gcgctgccga gcgccaggtc cacgatcgtc cgggaccgca gcagcgcgta
    14041 cagcgcgacg ttcagcagga cccccgagag catggcggag acgggcgacg gcgtccggct
    14101 gtgggcgtcc ggcagccacg ggtgcagcgg gacgaagccc accttcgtgc cgatgccgac
    14161 aaacagcagg acgaaggccc accggacgac ctgcgggtcg agggagccgg ccgcgcctcg
    14221 aagggccgac agcgacagcg cggagccgag cggcagcccg ccgcggacgc cggcgtaggc
    14281 gaggagcagc agcccgagca ggctcacgct gatgccgacc gagcagagga gcaggtactt
    14341 ccaggccgcc tcgacggccc cgtccttccg gtagagcgcc acgagcggcg tggtcgccag
    14401 cgtcgtgacc tcgagcgcga tccagagcac gccgaggtga tccgtcagca gcgtcacgag
    14461 catggcggcc acgaacaccg gcatcagcac gtaatagagc cggaccttcc gcagcggcag
    14521 gatgcccgcg tcgtgctcgg cgccgacgta gcgggtgctg gccgctgctg ccgagaccca
    14581 gaccattacc accaggccgg ccatcgtgac cgtgaaccga tcgagcatcc agtggccgcc
    14641 gaaccaccgt tctccggcgg cgaccaccgg ccacgccgcc gccgccagcg ctgccgcgcc
    14701 cgccaggctg gccgccaccg ctccgcgccc ggcgcttcgg gcatcgggcg ccgcggccgc
    14761 gaggaccgct gctgcggcgg gcacgaggag gagcagcagc aggctcaatc ggtcagctcc
    14821 ctgagcgcgt ccgtgtcgtg gctggcgtag agcgcgtgga cctgcccgct gaggacggcc
    14881 atcaggacca cgccgctggc ggcgacagcg aagatgccca tttccatcag gaacggcact
    14941 ccgcccagcg ccacgacggc gaacgccgag atcccgttct cgagcgtcag gaacccgatg
    15001 atctgcgagt acaggtcgcg ccggatgatg atgagcagga agcccagcag gaagaacacg
    15061 aggaaccacc cgagcgcccc act
//
LOCUS       DAOSIF010000018        14930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4130_length_14930_cov_3.690218, whole genome shotgun sequence.
ACCESSION   DAOSIF010000018 DAOSIF010000000
VERSION     DAOSIF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14930
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4130_length_14930_cov_3.690218"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..809)
                     /gene="lptB"
                     /locus_tag="PLT35_01445"
     CDS             complement(84..809)
                     /gene="lptB"
                     /locus_tag="PLT35_01445"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOG27916.1"
                     /translation="MATLQTDALTKTYDGRTVVRGVSVEVRSGEVVGLLGPNGAGKTT
                     TFYMTVGLTSPDSGRVLLDGVDVTRDPMYVRARKGIGYLPQEPSIFRGLTVAQNILAI
                     LETLGLRAAERRARLQDLLAELNLTPLANSPAYTLSGGERRRVEITRALVVSPQFILL
                     DEPFAGIDPIAVADIQAIVSHLSERGIGVLITDHNVRETLRITDRAYIVNEGVIFRSG
                     TPESLAADEEVRKVYLGADFRLD"
     gene            complement(810..2951)
                     /locus_tag="PLT35_01450"
     CDS             complement(810..2951)
                     /locus_tag="PLT35_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOG27917.1"
                     /translation="MRWQQRAQVALAAFVAGFVVLLLLAVRPGRPRESAPPPDASTGD
                     PAASAASTSGDVVNLLRDIENFRLSYDRLLTYPDGRQKLTGARILVPRRRGRDYEITA
                     REAEVGPGQDLIAMQGEVRLGATDGLAAGTDAATFSRSEGVLRAPGPASFSKGGMSGS
                     SVGLTYDQARDVMWLLDKAVTVFAPERAGEDGAEITAGAAVLARNDHYARYERGFTLV
                     SGARRMSSDAATAYLTADGARVESLDMRGRSRVTGLGEGPGSLGAMEADDITLEFTAD
                     GKALGAATLASRSREPARLDLGSAGAGHRRVSGRWIDIRFAPDGATVRELTVRESVRL
                     DLPEEDGRPAQTITAASLVSRAAGGGAIDGARFDGGVEYREASAPHGRLVRARALEIT
                     TAPGLGALDDARFDGGVRFEEDRLRGAAERARYLVAGGRIVLEGTDGTTGQVPRATDG
                     SVAIDAARIELALEPRSISARGDVRTAMAPAPAGHAGAAKREVRRAGLLDETQPVFAA
                     STALEYDGAAGLAVYTAEAPAQARLWQGDTTIQGDRLSLDDATGRLSAKGRVTSTILI
                     EQTNERTGAVERVHSVGSADEFLYEDDGRRATYTGSAHVSGPQGDLRASTIEVYLAAS
                     SRELERVEAYASVFLQEGARRASGDRLTYATAAGRYLMIGSPVKIEAECRETTGRSLT
                     FYKSTNNILVEPNEEFRTQVRTLPNCTPGRD"
     gene            complement(2948..4675)
                     /gene="recJ"
                     /locus_tag="PLT35_01455"
     CDS             complement(2948..4675)
                     /gene="recJ"
                     /locus_tag="PLT35_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOG27918.1"
                     /translation="MARYIWEVPPLDEAAAARLARDAGLDPVLARLLVARGVTDAGQA
                     GRFLHPSLDHLHDPFLMAGMAAATDRVLAAVAARERIAVHGDYDVDGITATVLVRRAL
                     EMLGGDVVHFVPERVRDGYGLNASAIERLHGEGVSVVVSVDCGIRGDEAARRARALGL
                     DLVITDHHEPGERLPDAVAVVNPKRRDCAYPDKHLAGAGVALKLVQALCRRTGHDAWL
                     PAFVKIAALGTLADAVPLLGENRVIAKLGLDALSRGPHKIGLRALIESAGLAGKAIDA
                     YHVSFVLAPRMNAAGRMSTPDIAMRLLLATDDDMAHEARELAARLDAENARRQQEERA
                     VFVEARRAVEKDPDVGSRHVLVVAGEGWHRGVIGIVASRLVDAFGKPAVVLAVEGDAV
                     HGSCRSIPAFDMLAGLQACEAHLEQFGGHRAAAGLTMRREAIRAFRQAIAAWAEERLS
                     PDDLRPRLRIDARIGFRQITPGLAQALSALGPFGIGNPKPVFATAGVRLADGPRRLKE
                     RHLSMSLRHDGVPLRAVFWRAAERAAEIAALAEGLDVAYSVQQNTFNGATSVEVSLAD
                     VRPAGGGVP"
     gene            complement(4675..5691)
                     /locus_tag="PLT35_01460"
     CDS             complement(4675..5691)
                     /locus_tag="PLT35_01460"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOG27919.1"
                     /translation="MPAELLDFEAPIGVLLKEIDALSLMPRTEARTRDIARLKRRVDE
                     MRRRLFAALTPWQCVQVARHPGRPGTLDYLDRLFTDFVEIHGDRRFADDPAIVCGMAR
                     YHGAPALVVGHQKGRTTKEKIARNFGYARPEGYRKALRAMRLAEKYARPIIVFVDTPA
                     AFPGIESEERGVAEAIAHNLREMSALAVPVIVVVSGEGGSGGALGIGVGDRVLIQQYA
                     IFSVIPPEGCSAILWRDSNRKVEAASALKLTAPDLLAAGIVDQVVPEPLGGAHQDAGE
                     AARLVDEALVRALADVSALSVKQRLEQRYDRLRRMGRPDVDFEAAAAGPSAAPPSRRP
                     SRSR"
     gene            complement(5722..9285)
                     /locus_tag="PLT35_01465"
     CDS             complement(5722..9285)
                     /locus_tag="PLT35_01465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG27920.1"
                     /translation="MAEFVHLHLHTEYSLLDGACRIDELLEEADRLGMRSLAVTEHGN
                     LFSAITFHDRARARGIKPILGCEVYVAPGSRLSKGGTPGEAAHHLILLAETNEGFRNL
                     VALTSSAYTEGFYYKPRIDKELLASHAKGLIGLSSCLKGEVPSELRADRVDRAAAAAA
                     QYRDILGEGHFFLELQDQGIPEQRRVNDGLLALSRRIGVPLVCTNDVHYLRQDDFRPH
                     DLLLCIGTGKVVSDEKRLRYFGDQFYLKTADEMAAVFGHVGGALRNTVAIADRCAVDL
                     SDGGPHLPNFQVPDGCTVDGYFEQVVRQGFESRLAQLELQRAAGLLRHPLEDYRARLQ
                     YEIDVITRMQYPGYFLITWDFVRYAREQGIPVGPGRGSAAGSLVAYCLRITDVDPLQF
                     DLLFERFLNPERVSLPDIDIDFCERRRGEVIEYVTQKYGRENVAQIITFGTMKAKAAV
                     RDVGRVLGMSVAEVDRVAKLIPPALDMTLDRALAETPALRQLEQGDARVSELLGAARR
                     LEGMARHASVHAAGVVIAPRPVTEYAPLYKGSHDEITTQWGMKEIERIGLLKMDFLGL
                     STLTLIHDALAQIASTTGERLVMEELPLTDARTYDLFCEGQTLGIFQFESAGMRQILR
                     QAKPRRFEDLIALNALYRPGPIKGGMIEDFVNRKHGRVAVTYEFEALRPILEETYGVM
                     AYQEQVMRVASTLAGFSLGEADLLRKAMGKKNQTLMQAQRVKFVDGAVTRGIAAGTAN
                     QIFDLIEQFASYGFNKSHSTAYALLAYRTAYLKANYPWHFMAALLTIESQNTAKLALY
                     LHECRELQVPILPPDVNSSDVAFTVRPEGVRFGLGAIKNVGASAIASILAVRERAGRI
                     ASLSRLCEDADLRLVNKRVLESLIKGGAMDGLGDAACAGAASPAHVRPRLLAAVDGAV
                     ESGARRQRDRERGQARLFGGPDGGGDAADDALPPADALSLPQQLAFEKEALGLYLSGH
                     PIDRYRGALADFGARSLGDLLGSGDDPPAEEDPEGAAGFPRAAVRIREDVAIGGVVAS
                     VRAITTKKGERMATFVLDDPDGSVETVVFPDAFARAAPQIQADALVLVKGRFERDEES
                     TRLFASEVLPIEAVRDHVVREVLVRLESPGCDRRACEDILGVLGRHRGDRRVAFLVRT
                     ASAGRPLLARVDVSGQLRVRPSEALVADLERICGPGSVTVR"
     gene            9497..10621
                     /locus_tag="PLT35_01470"
     CDS             9497..10621
                     /locus_tag="PLT35_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOG27921.1"
                     /translation="MAALGAGLAGACAGRLAPGAAAPVVAFPGGVLWQTPLPAAPAHA
                     PAYDELRAYVALRDRAVVAVDHASGAVAWIAQAAATVAPVRVGGLLVAADGTRAWALD
                     AATGHARWQQTLPAEAARAPVASGSGVLFATRSGEVVLLSLDDGRPAWRTTVGASPVA
                     LVAGREGRSYLGFEDGRLTALAGDGSVSWNRALPGTPLSLTPVADRVFVGSADRFLYS
                     LKEKSGAVAWRWRTGGSVTAQVAADRRRVYFVSRDAMVRALDRRHGDLRWQRPLRGRA
                     VGAPLLAGDTVIAAGVAPELRGFRIADGAAAGVAPLPGWAVDGPFLAPAAADVPARVI
                     VVTAGGQMLAVGQTVEPPIVPLERVPGRRIVLGARPPAAP"
     gene            complement(10635..11603)
                     /locus_tag="PLT35_01475"
     CDS             complement(10635..11603)
                     /locus_tag="PLT35_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27922.1"
                     /translation="MLAGVNVKIALKRGALVAAANWQVTIVQFLAESAFKLLLSVPVI
                     GGVLLVVVVLGRELPELFSPHVRESVSYLATALAAEPPALVAFLASLAIAGLSGSVLM
                     FIVKGGTVAVLVEGERVAGAVEAPPLRLNAVLAAARFSIDRFEAGCRRFMRRFLRLGG
                     LLILVYVVAGAAYLGLILAWAEPAPEGQSDWTAKAGLLSGALVAAITLVNFFYLLIQM
                     VVVADDCSVRTAALRMAAFVRAEARLAFGVFGVMLLVVVGATLISLIATTSLGVIAFV
                     PFAGLAVVPLQVAAWLFRELVFQYLGLSALGAYAGLYRRHASAQRK"
     gene            11678..>14930
                     /locus_tag="PLT35_01480"
     CDS             11678..>14930
                     /locus_tag="PLT35_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOG27923.1"
                     /translation="MLHSAVRTTFPFRPWRLLAACLLSAAAVGALGAAYEYRHLGWSD
                     AEAERRVADEVRREFDALTQELDRAAASVAVAHGLIARAARDVGAVHALFDTLDSPAR
                     RGVSVTAYAGDGTPLAWSGRPSDLPRARVTGGEALFVAPGSLGPRLVRVHPVTDAAAR
                     VGTVVAEAPLPSIGPSPGLAADTLAWNTSLVPVMLRATYEGAGESRAPGVFVLPGTGG
                     RALLEGRVTSRGVEAARRAVRGQTKAAVLGVCAVFLAWGIAPVLSWARRARRPAAVST
                     AMCLIAGLIVGARGVAWLAVPMAGTVVPMALSGGDRWYTRLWMRSPADFLLSAAAALA
                     LVVLAGAAASRARIGLRRARVAPDESRTAALAFVLAHLAGGTAASMWLARYVFIGSSL
                     TGVSAIEWLRFALYPVDGAKTAVAMGLIFWHAAAVWGAATILRLASIWWRTAPRSAWR
                     AVVVAAWAAPAALVAARAGALGPGNPTWVLGPPVVLSMALAWGLPRLLRWRRHSSQAA
                     AFLALFAALALPSVAFYPAAFAQADRATRTLIESDLAPQATRQRDDVQARVRRAMNQV
                     DAVPGLNEFVRGQGPAPGGQVPTEAAFLVWSQTDLAAYRLTSAIELYGGDGGIVSRFA
                     LNLPEYTQAQQRWRESGCDWDLFEEVSPFGSEERRLLHAGRALCTEEDGRIRHVGAIV
                     IHAMLDYGSLPFIMSQNPYVELFRDQRAGEADAPALDVQFTVYGWSLRPIYTSGGAAW
                     TLDRSTFERVYASRTPFWTRQASAGGLDEVYLANDRGGIYALGYRVETPIGHLVRVAE
                     LLTLTGVCFVLLAIVAATGFALAGVPGENGRALLQEIRASFYRKLALAFVAVAVIPVL
                     TLAFVSRAYMTGRLYADIEEAAIQTITVAQRFVQDYGRLQERSEGAPGMLTDDVMVEI
                     ARVINQDVNVFEGPRLVATSERDLFASGLLATRTPADVYRAVVLDRRASYVGRERVGQ
                     FSYIVAAAPVRVSGTNALLTVPLTLRQRAIEREIDDLDRRILLAALAFILLGSVLGYW
                     IAERIADPVNRLQRASARLARGDLSTRVALTSSDELRRLVATFNGMAADLQRQQ"
BASE COUNT         1980 a         5494 c         5280 g         2176 t
ORIGIN      
        1 gatcagcttc tgaacgagct tcgtgtggag tcgttgctgc agcgccatga gtcgtcaccc
       61 ggagtgtagt gcctcggtcg ccctcagtcc agccggaaat ccgccccgag atagaccttc
      121 cggacctcct cgtccgccgc caggctctcg ggcgtgccgc tgcggaagat cacgccttcg
      181 ttcacgatgt aggcccggtc cgtgatccgc agcgtctcgc gcacgttgtg gtcggtgatc
      241 agcacgccga tgccccgctc gctcaggtgc gacacgatcg cctggatgtc ggccaccgcg
      301 atcggatcga tcccggcgaa cggctcgtcg agcaggatga actgcggcga gaccacgagc
      361 gcgcgggtga tctccacgcg ccggcgctcc ccgcccgaga gcgtgtaggc cggcgagttg
      421 gccagcggtg tcaggttgag ctccgcgagc aggtcctgca ggcgcgcccg ccgctcggca
      481 gcccggaggc ccagcgtctc caggatcgcc aggatgttct gcgccaccgt cagcccgcgg
      541 aagatcgacg gctcctgcgg caggtagccg atgcccttgc gcgcgcggac gtacatcggg
      601 tcccgcgtca cgtcgacccc gtcgagcagc acccgccccg agtcgggcga ggtcaggccg
      661 acggtcatgt agaacgtcgt cgtctttccc gccccgttcg gaccgagcag gccgaccacc
      721 tcgcccgagc gcacctcgac gctgacgccc cggacgaccg tccgcccgtc gtacgtcttc
      781 gtcagcgcgt ccgtctgcag cgtcgccact tagtcccgcc ctggggtgca gttcgggagc
      841 gtccgcacct gcgtgcggaa ctcctcgttg ggttcgacga ggatgttatt ggtcgacttg
      901 tagaatgtca aagagcggcc ggtcgtctcg cggcactccg cctcgatctt caccggcgag
      961 ccgatcatca ggtagcggcc ggcggccgtc gcgtaagtga gccggtcgcc gctggcgcgt
     1021 cgcgcgccct cctgcaggaa gacgctcgcg tacgcctcga cgcgctcgag ttcgcggctc
     1081 gacgccgcca gatacacctc gatcgtcgac gcgcggagat cgccctgcgg ccccgagacg
     1141 tgggccgagc cggtgtacgt cgcccgccgt ccatcgtcct cgtacaggaa ctcgtcggcg
     1201 ctgccgacgg agtgcacccg ctcgaccgcc ccggttctct cgttcgtctg ctcgatcagg
     1261 atcgtcgagg tcacgcggcc cttggcgctc agcctgccgg tcgcatcgtc caggctgagg
     1321 cggtcgccct ggatggtcgt gtcgccctgc cagagcctcg cttgggccgg cgcctcggcc
     1381 gtgtagacgg cgaggccagc cgcgccgtcg tactccagtg ccgtcgaggc ggcaaacacc
     1441 ggctgcgtct cgtcgaggag gccggcccgg cggacttccc gcttcgccgc gcccgcatgc
     1501 cccgctggcg ccggcgccat ggccgtccgg acatcgccgc gcgccgagat gctccggggc
     1561 tcgagggcga gctcgatccg ggcggcgtcg atggccaccg agccgtccgt cgcccgcggg
     1621 acctggccgg tcgtcccgtc ggtcccctcg aggacgatcc ggccgcccgc cacgagatag
     1681 cgcgcccgct cggcggcgcc ccgcagcctg tcttcctcga agcggacgcc cccgtcgaac
     1741 cgggcgtcgt cgagggcgcc gagccctggc gccgtcgtga tctccagcgc ccgcgcacga
     1801 accagccgtc cgtgcggcgc cgacgcctcc cggtactcca cgcccccgtc gaaccgggct
     1861 ccgtcgatgg ccccgccgcc ggccgcacgc gacacgagcg aggccgcggt aatcgtctgg
     1921 gccggccggc catcctcttc cgggaggtcg aggcgcacgg actcccggac cgtgagctcg
     1981 cgcaccgtgg cgccgtcggg tgcgaacctg atgtcgatcc agcgccctga gacgcgcctg
     2041 tggcccgcgc cggccgagcc gaggtcgagg cgcgccggtt cccggctcct gctcgccagc
     2101 gtggcggcgc ccagggcctt tccgtcagcc gtgaactcga gcgtgatgtc gtctgcctcc
     2161 atcgccccga gcgagcccgg cccctcgccg agcccggtca cgcgagaccg gccccgcatg
     2221 tccagcgact cgacgcgcgc accgtccgcc gtcaggtagg ccgtggccgc gtcgctgctc
     2281 attctcctcg cgcccgacac gagcgtgaag ccgcgctcgt accgcgcgta gtggtcgttg
     2341 cgcgccagca cggcggcgcc ggcggtgatc tcggccccgt cctcgccggc gcgctcgggc
     2401 gcgaacacgg tcaccgcctt gtccagcagc cacatgacgt cccgcgcctg gtcgtacgtc
     2461 aggccgacag atgaccccga catgccgccc ttcgagaagg acgccggccc gggggcgcgc
     2521 agcacgccct ccgaccggct gaacgtcgcc gcgtcggtcc cagccgccag cccgtcggtc
     2581 gcgccaagcc ggacctcgcc ctgcatcgcg atcaaatcct gccctgggcc gacctcggcc
     2641 tcgcgggcgg tgatctcgta gtcgcggccg cgcctgcgtg gcacgagaat cctggcgcct
     2701 gtcagcttct ggcggccgtc ggggtacgtg agcagccggt cgtaggacag ccggaagttc
     2761 tcgatgtccc gcagcaggtt cacgacgtcg cccgacgtgc ttgcggccga cgcggccggg
     2821 tcgccggtcg aggcgtccgg cggaggcgcg ctctcccgcg gccgccccgg gcgcacggcc
     2881 agaaggagaa gcacgacgaa gcccgccacg aacgcggcga gcgcgacctg cgcgcgctgc
     2941 tgccacctca cggaacaccg ccgcccgccg gacggacgtc cgcgagggag acctcgaccg
     3001 acgtcgcgcc gttgaaggtg ttctgctgca ctgagtacgc cacgtcgagg ccctcggcga
     3061 gggcggcgat ctcggcggct cgctcggccg cccgccagaa gacggcccgc agcggcacgc
     3121 cgtcgtgccg gaggctcatg ctcagatggc gttccttgag tcggcgcgga ccgtcggcga
     3181 gccggacgcc ggcggtcgcg aacacgggct tggggttgcc gatcccgaaa ggcccgagag
     3241 cgctcagcgc ctgggcgagc cctggggtga tctggcggaa cccgatccga gcgtcaatcc
     3301 gcagccgggg ccgcaggtcg tccggcgaca gccgctcctc agcccaggct gcgatggcct
     3361 gcctgaacgc ccggatggcc tcccgccgca tcgtcaggcc ggcggcggcc cggtgcccgc
     3421 cgaactgctc gagatgcgcc tcgcacgcct gcaggcccgc gagcatgtcg aaggcgggga
     3481 tgctccggca ggagccgtgg accgcatccc cctcgaccgc cagcacgact gcgggcttgc
     3541 cgaacgcgtc gacgagccgc gaggcgacga tcccgatcac gccccggtgc cagccctcac
     3601 cggcgacgac gagcacgtgc cgcgagccga cgtccggatc cttctcgacc gcccggcgcg
     3661 cctcgacgaa gactgcgcgc tcctcctgct gccggcgcgc gttttcggcg tcgagccggg
     3721 ccgccagctc gcgcgcctcg tgagccatgt cgtcgtcggt ggccaggagg agccgcatgg
     3781 cgatgtccgg cgtgctcatc cgccccgcgg cgttcatccg cggcgcgagc acgaaggaga
     3841 cgtggtaggc gtcgatggcc ttgccagcga gccctgcgct ttcgatgagc gcgcgcaggc
     3901 cgatcttgtg cgggccccgg gacagcgcgt cgagtcccag cttcgcgatg acgcggttct
     3961 cgccgagcag cggcacggca tccgccagcg tgccgagcgc tgcgatcttc acgaaggccg
     4021 gcagccaggc gtcgtgcccc gtgcgccggc acagcgcctg caccagcttg agggccacgc
     4081 cggcgccggc caggtgcttg tccgggtacg cgcagtcgcg tcgcttcgga ttcacgacgg
     4141 ccacggcgtc gggcaggcgc tcgccgggct cgtggtggtc ggtgatcacc aggtccaggc
     4201 cgagcgcccg tgcgcgcctg gccgcctcgt cgccgcggat gccgcagtcg accgagacga
     4261 cgactgagac gccttcgccg tgcaggcgct cgatggcgga cgcgttcagg ccgtagccgt
     4321 cccgtacccg ctcgggcacg aagtgcacga cgtcgccccc cagcatctcg agggcgcggc
     4381 ggacgaggac tgtggcggtg atgccgtcca cgtcgtagtc gccgtgcacg gcgatgcgct
     4441 ctcgcgcggc gacggcggcc aggactcggt ccgtcgcggc ggccatcccg gccatcagga
     4501 acgggtcgtg caggtggtcc aggctggggt ggaggaagcg gccggcctgg ccggcgtcgg
     4561 tcacgccgcg ggcgacgagc aggcgcgcca ggacggggtc gaggccggca tcccgggcca
     4621 gccgcgcggc tgctgcctca tcgagcggcg gaacctccca gatgtaccgg gccatcagcg
     4681 agagcgggag gggcggcgcg atggcggcgc cgcagagggc cccgccgccg ccgcctcgaa
     4741 atcgacgtcc gggcgcccca tgcgcctgag ccggtcgtag cgctgctcga gacgctgctt
     4801 cacgctcagg gccgacacgt cggcgagcgc cctgacgagg gcttcgtcaa ccaggcgcgc
     4861 cgcctcgccc gcgtcctggt gtgcgccgcc cagcggctcc ggtaccacct ggtcgacgat
     4921 gccggcagcg agcaggtcgg gcgccgtcag cttcagcgcc gacgccgcct ccactttccg
     4981 gttcgagtcg cgccacagga tcgccgaaca gccctcaggc gggatgacgc tgaaaatcgc
     5041 gtactgctgg atcaacacgc ggtcgccgac accgatgccg agcgcgccgc cgctaccgcc
     5101 ctcgccgctg acgaccacga tcacgggaac cgccagggcg gacatttccc gcaggttgtg
     5161 ggcgatcgcc tcggccacgc cccgctcttc ggattcgatg ccagggaagg ccgccggcgt
     5221 gtccacgaac acgatgatcg ggcgcgcgta cttctcggcc aggcgcatcg cccgcagcgc
     5281 cttgcggtag ccctccggcc gcgcgtaccc gaagttgcgc gcgatcttct ccttggtcgt
     5341 gcgtcccttc tgatgcccga cgacgagcgc cggcgcgccg tggtagcggg ccatgccgca
     5401 cacgatggcc gggtcgtcgg cgaaccgtcg gtcgccgtgg atctcgacga aatcggtgaa
     5461 gaggcggtcg aggtagtcca gcgtcccggg ccggccgggg tgccgggcca cttgcacgca
     5521 ttgccacgga gtcagcgcgg cgaacaggcg ccggcgcatc tcgtccaccc ggcgcttcag
     5581 gcgtgcgatg tcgcgcgtgc gcgcctcggt ccgaggcatc aggctcaggg cgtcgatctc
     5641 cttgagcagg acgccaatcg gggcctcgaa gtcgagcagc tcagccggca tctcgtcacc
     5701 agggtccgtg tccgttcgat cctaccgcac ggtgaccgag cccggaccgc agatccgttc
     5761 gaggtcggcc accagcgcct cggacggccg cacccgcagc tgccccgaca cgtccacccg
     5821 ggccagcagg gggcggccgg cggaggccgt tcgcaccagg aacgctacac gccgatctcc
     5881 cctgtggcgc cccaggacgc cgaggatgtc ctcgcaggcg cggcggtcgc accccggcga
     5941 ctccaggcga acgagcacct cgcggaccac gtggtctcgg accgcttcga tcggcaggac
     6001 ctcggatgcg aagaggcgcg tcgactcctc gtcccgctcg aagcgccctt tcaccagcac
     6061 cagcgcgtct gcctggatct gcggcgcggc cctggcgaag gcatccggaa agacgacggt
     6121 ttcgacggat ccgtccgggt cgtcgaggac gaacgtggcc atccgctcgc ctttcttcgt
     6181 cgtgatggcg cggacgctcg ccacgacgcc cccgatcgcg acgtcctccc tgatgcgaac
     6241 cgcggcccgc gggaagccgg ccgcgccctc cgggtcttcc tccgccggcg ggtcgtcgcc
     6301 gctcccgagc agatccccga ggcttcgcgc gccgaagtcg gcgagcgcgc cccggtagcg
     6361 gtcgatcggg tggccgctga ggtagagccc gagcgcctcc ttctcgaacg ccagctgctg
     6421 cggcagcgac agcgcgtcgg cgggcggaag cgcgtcatcc gccgcgtcgc cgccgccgtc
     6481 cggccctccg aagaggcgcg cctggccgcg ttcgcggtcg cgctgccgcc gcgcgccgga
     6541 ctccacggcg ccgtcgaccg cggccaggag ccggggtcgc acgtgcgcgg ggctggccgc
     6601 gcccgcgcag gccgcgtcgc cgagtccgtc catggcgccg cccttgatga ggctctcgag
     6661 cacgcgcttg ttcacgagcc gcaggtccgc atcctcgcac aggcgggaga gcgaggcgat
     6721 gcgtccggcg cgctcgcgga ccgcgaggat cgacgcgatc gcgcttgcgc ccacgttctt
     6781 gatcgcaccg aggccgaaac gcacgccctc gggcctgacg gtgaacgcca cgtcgctgct
     6841 gttgacgtcc ggcggcagga tcggcacctg cagctcgcgg cactcgtgca ggtacagggc
     6901 gagcttcgcg gtgttctgag actcgatcgt cagcagcgcc gccatgaagt gccaggggta
     6961 gttcgccttg aggtaggcgg tccggtaggc cagcagcgcg taggcggtgg agtgggactt
     7021 gttgaacccg tagctcgcga actgctcgat gagatcgaag atctggttcg cggtccccgc
     7081 cgcgatgccc cgcgtcacgg ctccgtccac gaacttgacg cgctgcgcct gcatgagcgt
     7141 ctggttcttc ttgcccatcg ccttgcgcag caggtcggcc tcgcccaggg agaaccccgc
     7201 gagagtgctc gcgacgcgca tgacctgctc ctggtaggcc atgacgccgt aggtctcctc
     7261 caggatcggc cgcagcgcct cgaactcgta cgtgaccgcc acgcggccgt gcttgcggtt
     7321 gacgaagtcc tcgatcatgc cgcccttgat cgggcccggg cggtagagcg cgttcagggc
     7381 gatgagatcc tcgaaccgcc ggggcttcgc ctggcgcagg atctggcgca tgccggcgct
     7441 ctcgaactgg aagatcccca gggtctggcc ctcgcagaac aggtcgtacg tcctggcgtc
     7501 ggtgagaggc agctcctcca tgacgagccg ctcgcccgtc gtcgaggcga tctgcgccag
     7561 cgcgtcgtgg atgagcgtca gcgtgctgag gcccaggaag tccatcttca ggaggccgat
     7621 ccgctcgatc tccttcatgc cccactgagt cgtgatctcg tcgtggctgc ccttgtagag
     7681 cggcgcgtac tccgtcaccg ggcgcggcgc gatcaccacg ccggcggcgt gcaccgacgc
     7741 gtggcgggcc atgccttcga ggcgccgggc cgcgccgagc agttccgaca cccgcgcgtc
     7801 gccctgctcc agctgccgca aggccggagt ctctgcgagg gccctgtcga gggtcatgtc
     7861 gagcgccggc gggatcagct tcgctacccg atcgacctcc gcgacgctca tgcccagcac
     7921 gcgccccacg tcgcgcacgg ccgccttcgc cttcatcgtt ccgaaggtga tgatctgcgc
     7981 cacgttctcc cggccgtact tctgcgtgac gtactcgatc acctcgccgc ggcggcgctc
     8041 gcagaagtcg atgtcgatgt ccggcagcga cacgcgctcg ggattgagga accgctcgaa
     8101 cagcaggtcg aactgcagcg ggtccacgtc ggtgatgcgc aggcagtacg ccaccaggct
     8161 ccccgccgcc gatccgcgcc cggggccgac gggaatcccc tgctcccgcg cgtagcggac
     8221 gaagtcccag gtgatgagga agtagcccgg atactgcatg cgcgtgatga cgtcgatctc
     8281 gtactgcagc cgggcacggt agtcctcgag cgggtgccgc agcaggccgg ctgcgcgctg
     8341 cagctccagc tgcgccagcc gactctcgaa gccctgcctg accacctgct cgaagtagcc
     8401 gtcgacggtg cagccgtcgg gcacctggaa gttcggcagg tgcgggcccc cgtccgacag
     8461 atcgacggcg caccgatccg cgattgcgac cgtgttcctc agcgcgccgc cgacgtgccc
     8521 gaagaccgcc gccatctcgt cggccgtctt gaggtagaac tggtcgccga agtaccgcag
     8581 ccgcttctcg tcgctgacga ccttgcccgt gccgatgcac aggaggaggt cgtgggggcg
     8641 gaagtcgtcc tggcgcaggt agtgcacgtc gttggtgcac acgaggggca cgccgatcct
     8701 gcgcgacagc gcgagcagcc cgtcgttcac gcgccgctgc tccgggatgc cctggtcctg
     8761 caactcgagg aagaagtggc cctcgcccag gatgtcgcgg tactgcgccg ccgccgccgc
     8821 cgcccggtcg acgcggtcgg cccgcagttc gctcggcact tcccctttga ggcagctgct
     8881 cagcccgatg aggcccttgg cgtgggacgc gagcagttcc ttgtcgatcc gcggcttgta
     8941 gtagaagcct tcggtgtagg cggacgaggt cagggcgacg aggttgcgga acccctcgtt
     9001 cgtctccgcc agcaggatga ggtgatgggc cgcctcgccc ggagtccccc ccttcgacag
     9061 ccggctcccg ggagcgacgt acacctcgca cccgagaata ggcttgatgc ccctggcgcg
     9121 ggccctgtcg tggaacgtga tcgccgagaa caggttgccg tgctcggtga cggcgagaga
     9181 gcgcatgccg agccggtccg cctcctcgag gagctcgtca atccggcagg cgccgtcaag
     9241 cagggagtac tcggtgtgga gatgcagatg gacgaactcg gccatgtcgc gctcaacggg
     9301 tcaccggccg cggctcaccg cacagccgcg ccgaagggcc cccgacggcc agcggccgga
     9361 cagcgggagc aacgatggat cggtctgtca cgctggtcca ctcggggatg gcaggcgtgc
     9421 tgtgacgtat tataccgggg tgctgcagcg agcggggacg acggcttcct gtcgcggagc
     9481 gtgccgggcg ctggctgtgg ccgcgctcgg cgccgggctc gcgggcgcgt gcgccggcag
     9541 gctcgcgcct ggcgccgcgg cgcccgtcgt cgcgttcccc ggcggcgtgc tttggcagac
     9601 gccgctgccg gcggcgccgg cgcacgcgcc cgcctacgac gagttgcgcg cctacgtggc
     9661 cttgagagac cgggccgtgg tggcggtcga tcacgcctcg ggagccgtcg cctggatcgc
     9721 gcaggctgcc gccaccgtcg cccctgtgcg cgtcggcggc ctcctcgtgg cggccgacgg
     9781 gacgcgggcg tgggccctgg acgccgcaac gggacacgcg cgctggcagc agaccctgcc
     9841 cgcggaggcg gcgcgggccc ccgtcgcgtc cgggtccggc gtgctgttcg caaccagaag
     9901 cggtgaggtc gtcctcctct ccctcgacga cggccggccc gcatggcgga cgacggtggg
     9961 cgcgagcccc gtggcgctcg tggcaggccg cgaaggccgc tcctacctcg gattcgagga
    10021 cggacggctg acagcgctcg ccggcgacgg gtcggtgtcc tggaaccgcg cgctgcctgg
    10081 aacaccgctc tcgctcacgc cggtggccga cagggttttc gtcggatcag ccgaccggtt
    10141 cctgtactcg ctcaaggaga agagcggagc cgtcgcgtgg cgctggcgca cgggcggcag
    10201 cgtgacggcc caggtggcgg cggaccggcg gcgtgtctac ttcgtctcgc gcgacgcgat
    10261 ggtgcgcgcc ctcgaccggc ggcacggcga cttgcgctgg cagcggcccc tccgcggccg
    10321 cgccgtcggc gcgccgctct tggccggcga cacggtgatt gccgccggcg tcgcgccgga
    10381 gctccgcgga ttcaggatcg ccgacggggc cgccgccggc gtcgcgccgc ttccgggctg
    10441 ggccgtcgac gggccgttcc ttgcgcccgc cgccgcagac gtgcccgccc gcgtcatcgt
    10501 cgtgaccgct ggcggccaga tgctggccgt cgggcagacc gtcgagcccc cgattgtgcc
    10561 gctggagcgc gtgcccggca gacggatcgt gctcggcgcg cgcccgcccg ccgcgccttg
    10621 aaggcctcgc gcgctcactt cctctgggcc gaggcgtggc gccggtagag cccggcgtac
    10681 gcgccgagcg cgctcagccc gaggtactga aagacgagtt cgcggaacag ccaggccgcc
    10741 acctggagcg gcacgaccgc gaggcccgca aacggcacga acgcgatgac gcccaggctc
    10801 gtcgttgcga tgagggagat cagcgtggcc ccgacgacga cgagcagcat cacgccgaag
    10861 accccgaacg ccagccgggc ttcagcccgc acgaacgccg ccatccgcaa cgcggccgtc
    10921 cgcacgctgc aatcgtcggc cacgaccacc atctgaatca gcaggtagaa gaagttgacc
    10981 agcgtgatgg ccgccacgag cgctccagag aggagaccgg ccttcgccgt ccagtcggac
    11041 tggccctctg gcgccggctc cgcccaggcc aggatcaagc cgaggtaggc ggcacccgcc
    11101 acgacgtaca cgaggatgag cagcccccca agccgcagga accgccgcat gaaacgcctg
    11161 cagcctgcct cgaaccggtc gatgctgaaa cgcgccgcgg cgagaaccgc attgaggcgc
    11221 agcggcggag cctccacggc cccggcgacg cgctcaccct cgacgagcac ggcgacggtg
    11281 ccgcccttca cgatgaacat cagcaccgat cccgacagcc cggcgattgc cagcgacgcc
    11341 aggaaggcta caagcgcggg aggttcggcc gcgagcgccg tcgccaggta gctcacgctc
    11401 tcccggacgt gcggggagaa cagctcgggc agttcccgcc ccagcacgac cacgaccagg
    11461 agcacgcccc cgatgaccgg cacgctgagg agcagcttga acgccgattc ggccaggaac
    11521 tggacgatgg tcacctgcca gttcgccgca gcgaccagag cgccgcgctt gagggcgatc
    11581 ttgacgttga cgccagccag catgccgcct gagagttata gcacgctttc ccgggcgtcc
    11641 tccgcggccc cgcttgtcgc accgtccggg cggggctatg ctgcactcag ccgtgcgcac
    11701 gacgttcccc ttccgtccct ggcgcctgct ggcggcctgc ctgctgtcgg cggccgcggt
    11761 cggcgccctg ggcgcggcct acgagtaccg gcacctaggc tggtccgacg ccgaggccga
    11821 gcggcgcgtg gcggatgagg tccggcggga gttcgacgcc cttacccaag agctcgaccg
    11881 cgcggccgcc tcggtcgcgg tggcccacgg cctgatcgcc cgcgccgcac gcgacgtcgg
    11941 cgccgtgcac gcgctgttcg acacgctcga ctcgccggct cgccgcggtg tctccgtcac
    12001 agcctacgca ggcgacggga cgccgctcgc ctggtcgggc aggccttccg atcttccccg
    12061 ggcccgcgtc accggcggcg aggccctgtt tgtggcgccg ggatccttgg gcccccggct
    12121 cgtcagggtc caccccgtca cggacgcggc cgcgcgcgtg gggaccgtcg tcgccgaggc
    12181 gccgctgccg tcgatcgggc ccagccccgg cctggcggcc gacaccctgg cctggaacac
    12241 gtcgctcgtg ccggtcatgc ttcgcgcgac gtacgaagga gccggcgagt cccgcgcccc
    12301 tggcgtgttc gtcctgcctg gcaccggggg ccgggctctg ctggaagggc gggtcaccag
    12361 tcggggcgtt gaggcggcca ggcgggcggt gcgcggccag accaaggccg ccgtcctggg
    12421 cgtgtgcgcc gtgttcctcg cgtgggggat cgccccggtc ctgtcgtggg cgcggcgcgc
    12481 tcgccgcccg gccgccgtct caacggccat gtgcctgatc gccggactca tcgtcggggc
    12541 gcgaggtgtc gcgtggctgg cggtcccaat ggccgggacg gtcgtgccca tggcgttgag
    12601 cggcggggat cgctggtaca cccgcctctg gatgcggtcg cccgccgatt tcctgctctc
    12661 cgcggcagcg gcattggccc tggtcgtcct ggccggagcg gctgcatcgc gcgccaggat
    12721 cggcctgcgt cgcgcgcgcg tcgcgcccga cgagtcgcgc accgccgcgc tcgccttcgt
    12781 actcgcgcac ctggccggcg gcaccgccgc ttcgatgtgg ctcgcgcgct acgtcttcat
    12841 cggcagttcg ctcaccggcg tgtcggccat cgagtggctg cggttcgcgc tgtaccccgt
    12901 cgacggcgcg aagaccgccg tcgcgatggg cttgatcttc tggcacgcgg cagcggtgtg
    12961 gggcgccgcg acgatcctca ggctggcctc gatctggtgg cgcacggcgc cgcgctcggc
    13021 atggcgggcg gtcgtcgtgg ccgcgtgggc cgctccagcc gcgctcgtcg ccgcgcgggc
    13081 gggcgccctg ggccccggca accccacctg ggtgctcggg ccgcctgtcg tgctgtcgat
    13141 ggcgctcgcg tggggcctgc cgcgcctcct ccggtggagg cggcattcgt cgcaggcggc
    13201 ggcgtttctc gcgctcttcg ccgcgctggc gctgccatcc gtcgcgttct atcccgcggc
    13261 gttcgcgcaa gccgaccggg ccacccggac tctcatcgaa tcggacctcg cgccgcaggc
    13321 gacccggcag cgagacgacg tgcaggcccg cgtgcgccgc gcgatgaacc aggtcgacgc
    13381 ggtgccaggc ctcaacgagt tcgtgcgcgg gcaaggccct gcgccgggcg ggcaggtgcc
    13441 gaccgaggcg gcgttcctcg tctggtcgca gaccgacctg gcggcctaca ggctcacgtc
    13501 cgcgatcgag ctgtacggcg gcgacggcgg catcgtcagc cgctttgctc tcaatctgcc
    13561 ggagtacacg caggcccagc agcggtggcg ggaatcgggc tgcgactggg acctgttcga
    13621 ggaagtgtcc ccgttcgggt ccgaagaacg ccgactcctg cacgccgggc gggcgctgtg
    13681 cacggaggag gacgggcgca tccgccacgt cggcgcaatc gtgatccacg cgatgctcga
    13741 ctacggctcg ctcccgttca tcatgtcgca gaacccgtac gtggagctgt tccgcgatca
    13801 acgagcgggc gaagccgacg cgccggctct cgacgtgcag ttcaccgtct acggatggag
    13861 cctgcggcca atctacacgt ccgggggcgc cgcctggacg ctcgatcgga gcacgttcga
    13921 gcgggtctat gcctctcgca cgccgttctg gacacgccag gcctccgcgg gaggtctcga
    13981 cgaggtgtac ctggccaacg atcggggggg gatttacgcc ctcggctacc gcgtcgagac
    14041 gccgatcggc cacctcgtgc gcgtggcgga gctgctgacc ctcactgggg tgtgcttcgt
    14101 gctgctcgcc atcgtcgccg caaccggctt cgccctggcc ggggtgcccg gcgagaacgg
    14161 ccgcgcgctg ctgcaggaga tccgcgccag cttctaccgc aagctggcgc tggccttcgt
    14221 cgcagtggcg gtcattccag tcctgacgct cgcgttcgtg tcgcgcgcgt acatgacagg
    14281 caggctgtac gccgacatcg aggaggcggc catccaaacg atcaccgtgg cgcagcgctt
    14341 cgtgcaggac tacgggcggc tccaggagcg cagcgaagga gcgcccggga tgctgaccga
    14401 cgacgtcatg gtcgagatcg cgcgcgtgat caaccaggac gtgaacgtct tcgaggggcc
    14461 tcgcctggtg gccacgagcg agcgcgatct gttcgcgtcg ggcctgctgg ccacgcggac
    14521 gcccgccgac gtgtatcggg cggtcgtgct cgaccggcgc gcgtcgtacg tcgggcggga
    14581 gcgggtcggc cagttctcgt acatcgtggc ggcggcgccg gtgcgggtgt cgggcaccaa
    14641 cgctctgctc acggtgccgc tgacgctgcg ccagcgcgcc atcgagcggg aaatcgacga
    14701 tctcgatcgg cgcatcctcc tggcggccct ggcgttcatc ctgctgggct ccgtgctcgg
    14761 ctactggatc gccgagcgga tcgcggatcc ggtcaaccgc ctccaacggg cgtcggcgag
    14821 gctggcgcgc ggcgatctct cgacccgggt ggccctcacc tcctctgacg aattgcgccg
    14881 cctcgtcgcg acgttcaacg gcatggcggc ggacctgcag cgccagcaga
//
LOCUS       DAOSIF010000019        14887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4147_length_14887_cov_3.845874, whole genome shotgun sequence.
ACCESSION   DAOSIF010000019 DAOSIF010000000
VERSION     DAOSIF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14887
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4147_length_14887_cov_3.845874"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLT35_01485"
     CDS             <1..848
                     /locus_tag="PLT35_01485"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG27924.1"
                     /translation="GTQRRSWPNVIEAIKELRAGIIGRPYFAKGWYTNNRKSIGVGAE
                     TPVPDWLDFGLWQGPAPRRPFRDNLLHYNWHWFWHWGTGEALNNGTHMLDLMRLGLGV
                     EFPTRVASSGGRFHFTDDWETPDTQVIVLDYAEKVSMAWEGRSCSGRKIDGSGAGALF
                     HGENGSLLIDGNTYLVQDLDEKVVREVKSGADVDPRNPANPADSLDVAHVRNFLDAIR
                     GTAPLAAEIREGHVSTLLCQLGNIAYRTGRSLVIDNKTGRIVGDPEAAGYWAREYEKG
                     WEMSL"
     gene            911..1870
                     /locus_tag="PLT35_01490"
     CDS             911..1870
                     /locus_tag="PLT35_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG27925.1"
                     /translation="MRRVLLVGALLCGAAADAQPADLRIGIIGLDTSHVIAFTKSLND
                     ASSPDHVPGGRVVAAFKGGSPDVPASATRVDKFTAELQSTYGVRIVGTIAELCDLVDA
                     VLLESVDGRVHLEQAREVLRRRKPLFIDKPMAAGFADASEIARLANEAGTPWFSSSSL
                     RFAPAYQQFLADPARGRVLGVEAHGPASLEPTNPGLFWYGIHAVETLYTLMGPGCVSV
                     AMTSNADYDLAVGLWEDGRIGTVKGLRKGHQAYGALVYGEKAVTYLAVTDVSYVPLVR
                     QIMAFFQTGAPPVPPAETLEIMAFMDAAERSRLGGGAPVQLRR"
     gene            complement(1965..2165)
                     /locus_tag="PLT35_01495"
     CDS             complement(1965..2165)
                     /locus_tag="PLT35_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HOG27926.1"
                     /translation="MPNVHVDGPPIEDLGVKRALAREITDAMEKAYGYPRPAYVVIIR
                     ENRPENVSVGGELLCDRAAGGR"
     gene            2445..2732
                     /locus_tag="PLT35_01500"
     CDS             2445..2732
                     /locus_tag="PLT35_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27927.1"
                     /translation="MDRSSSVAEAVAVQGGRILARGHPGGRDPQGRPRLGHVRPRFDQ
                     SQPPGLDQAVYVREAIRMCPIEGAWLERKEGLKGSIEKGKLADAGNVSKIG"
     gene            2739..4562
                     /locus_tag="PLT35_01505"
     CDS             2739..4562
                     /locus_tag="PLT35_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOG27928.1"
                     /translation="MRKATAPVAALALSAAFAVACAGRPPRSVAHWDEDLLSPQRYRV
                     RIEKDVQVPMRDGVTLATDLYIPEAPGRFPALLWRNCYGKDGYGDMSWYASRGYVVVH
                     QDVRGKHASGGDFVHFRDDANDGFDTDEWIGAQPWSNGKIGLMGGSYLGYTGIAQAVR
                     GSRHLAAMSISVATGDVYNGWIYSDGALFLGFAFPWGAVGMNGREFRFQNPVPVPWPE
                     IFAHLPLETMDVKAGYPNPVYREWLRHPRAGDPYWKDVSYEKDIARISIPVLVLEGWY
                     DLFLRGALQDHVLIARDSLSARARRHKHLVIGPWAHEGGVRNNAPKAPAAGPDRSLDF
                     GPEAAVETRKIYLRWQDHWLKGIDNGIDREPPVRIFVMGENTWRFENEWPLARTRYTK
                     YYFGGTRANSINGDGTLSTAAPAAEQAADSYVYDPASPVPTLGGNTCCSQVPSGPWNQ
                     IEAEMRDDVLVYSTPELKTPLEVTGPVKVTLYASSSARDTDWTAKLVDVHPDGYAQNL
                     CDGIIRARYRAGRTAPADLLEPGRIYEYEIDLWATSNVFLPGHRIRVEISSSNFPRFD
                     RNLNTGEDPMTGTRMAKAEQTIYRSKQRPSHIVLPIIPRRR"
     gene            complement(4631..5197)
                     /locus_tag="PLT35_01510"
     CDS             complement(4631..5197)
                     /locus_tag="PLT35_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG27929.1"
                     /translation="MELLDAIRERRTVRSFRPDPVPEETVMAVLEAGTWAPSHGNKQP
                     WEFVLIGPATRAGLAERFREILEAGLLRSPELPEARKQAARAFAVDFGGAPLLFAVVC
                     PPATTDLDRYDFPLGAAAALQNLTLAAWEKQLGAIWLSIGSNPLVRELLGVGPDSTIA
                     GIVAMGVPENVPPPQPRVPVAEKLRRLP"
     gene            complement(5211..>5812)
                     /locus_tag="PLT35_01515"
     CDS             complement(5211..>5812)
                     /locus_tag="PLT35_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27930.1"
                     /translation="IMVPERYPEMVTRAAEYGNHRITIGAHYAMDVLGGRAVALHALA
                     HLLANDPRYVGRSFEKASTITDFRAAVNAARADVRAQLEAACGKAVAECAREDHGRFG
                     DSAANAAFYASTQTYGLPVVHPEQAIARPDVGRVAPEAGHLLTAAFPSLSLGEANGIL
                     TRTLGPGGGFLDDGSAFGIYARLDLYAASRYAAALTAGR"
     assembly_gap    5813..5822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5823..6572)
                     /locus_tag="PLT35_01520"
     CDS             complement(<5823..6572)
                     /locus_tag="PLT35_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27931.1"
                     /translation="MSRRILLGALALAVLLPSQAAGQSAATMPVLKGLAPVTALTRTR
                     AGLAALGANYTVTGGIQTGDWRQPTLLPFAEDQQLALRDAFSTHGNLTQLADGLGTTL
                     GSAYEARAHYIDRDRFTNVSPAVAGVIAYALSAAREDSDTGKYFFANLTTDGKAPVST
                     EARAILEQIGGLPDIFGRAYRVPAGSAGGGAYGDARPFQTEPVFTRIVGRDYFGVPTD
                     NTVLNRGPVMDLVNSPSYPSGHTTYGYVGSVL"
     gene            6699..6872
                     /locus_tag="PLT35_01525"
     CDS             6699..6872
                     /locus_tag="PLT35_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27932.1"
                     /translation="MTRWPVLVLVAIGIGADGYLHMLVAARLRHVAATRWGSRRYLAA
                     EWLLTDDARAKIA"
     gene            complement(7076..8098)
                     /locus_tag="PLT35_01530"
     CDS             complement(7076..8098)
                     /locus_tag="PLT35_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG27933.1"
                     /translation="MSQPLSALLVITLLAVTAAPEAQREDLQAYEPTVRVSGTIRAWG
                     NDRMKPLMALWEAGFKRHHPAARFETSLMGTGTGIAGLYTGVADIALMGRPADSTETM
                     AFEWVFRYRPLGVQVSTGSLGVPGKSFAPVVFVHKDNPISQLTLAQLDAVFGSEHRRG
                     QANARTWGDLGLGGSWAARPIRVYAPDIQTATGSFFREAVLKGSYKWNCDMTEFHDVR
                     RPDGSSHDGRQRVLDALAGEPGGIGLSALAYAGAAVKPLALSAGNEGPFYAPTPENLA
                     ARRYPLHRATWIFLNRPPGRPLDPAIREFLRYILSRDGQRDVERAGDYLPLTASVVRS
                     QLGLLE"
     gene            complement(8139..9020)
                     /locus_tag="PLT35_01535"
     CDS             complement(8139..9020)
                     /locus_tag="PLT35_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG27934.1"
                     /translation="MGELVSAWQTGFQRHQPGIRFDDRMHGTASAIGALYAGVGDVAI
                     LGREILRSELAAFERVKGYRPLGIDIATGSLDVRNFDFALVVFVHKDNPLVQLTLADL
                     DGVFGCEHRRGSANIRTWGQLGLAGEWAGRPIQTYGFALSRGFALFFQDTVMGGSGRW
                     NPGLREYVDVKRPDGTLLDSGQQVLDSLGQDRYGIAVSSLLYATPLVKPLALATDPAG
                     PAYHATKENLISRRYPLTRFITAFVDRPPGAAVDPKVDEFLRYILSREGQDAIVRDGR
                     YLPLSTEAVRDQTLRLQ"
     gene            complement(9274..10497)
                     /locus_tag="PLT35_01540"
     CDS             complement(9274..10497)
                     /locus_tag="PLT35_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27935.1"
                     /translation="MRHYLHVGLLLAAGVALVLVSTASGHSPSTPATEQQQGGSTAGM
                     AMQEARAEALKTKGKKAYYTRTFDLSGIPAYKPQQRVSGTLRMWGSNYPADGMLAGYW
                     EEAFRKFHPEIAFDFRLKATSGTIPGLVSGQSDLGPSVKITFPNLQFYQRYLNADPFE
                     IVYATGSYDVTGWSPGYGIVVHRENPLARITLEQLDGIFGAERLGGWVGTDWHPEFAR
                     GPERNIRSWGQLGLTGEWADKPIIPYGLNLRYHQATVISDRVLKGSDKWNERLRIYAN
                     YVTPDGRLSRGLNEDLLKDKYGIAYIAAPTVTMNFAAQEQVSPRLKILPLAETAAGPY
                     VEYTIETLQNRTYPLFDEVYFYTHALPGKGLDSKVREFLRFVLSREGQELIMKDGKYL
                     PLTAKVAREQLAKLD"
     gene            complement(10599..13796)
                     /locus_tag="PLT35_01545"
     CDS             complement(10599..13796)
                     /locus_tag="PLT35_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOG27936.1"
                     /translation="MSTRRSWETGLATGARRAKIAAVLALVCCAMCFSVPVLAQGSGG
                     TVNGTITDPSGAAIPKADVILVHQDTGAVRTILTNDRGFFSVPNLASGRYDLTVAATG
                     FATAVQEGVSVDVGSTVVANIRMQISSVEEKVDVVAATADIALSSSTLSTVVKGDVVR
                     ELPLNGRDWTMMATLEPGVHAIEAQADLSGGGNARANRGWGTQMSFGGNRPQQNNYRL
                     DGISINDYSGGGPGGVLGSVLGVDAIGEFSVVTGNASADYGKTSGGVINAVTRSGSND
                     FHGSAYGFFRNDAFDAPNYFDDGEKPDFYRNQYGAALGGPVVKNKLFFFADFEGLRQD
                     LGTTDLITVPSVAAREGRLESGAVTVSPLVAPYLGIYPLPNRGESGDVGTYSFVSNSQ
                     SRDDLYTARVDYNPSASAMLHGSFLVDRSHREGPNESGGVIVGATSNRAMGSLEYTRI
                     FSSNLVSVSRVGFSSSESHAPEWIENLNPLFDDLSLGSVPGNPMGTIEVSGLTGVDGG
                     KGGVGESFHSYKSLQIYNDLIWTKGVHVIKVGVAFERNYYDTTTSNAPNGRWVFGSLR
                     SFLTNQPTSFTASIQGKNPTMYLAQNVFGVYVADDFRVRPNLTLNLGLRYEPVGLPTE
                     KEDRFSNLDNITDPAPRIGSPYFNNPTKWNISPRLGFAWDPFSNGKTAIRGGVGVYDT
                     LPLLYQLSLLIVNTQPFFRTGTLTGLKADTFPTAAYGMLTANDTRAAYIQKDPDRSYV
                     TQWNVNVERQLPASFVVHVGYVGQHGVHQPFRTNDSNIVVPERLPDGSLQWPLPLKSG
                     KKLNPNVGVINTLLWIGKNDYNALNVGLRRQAGGLRAGVSYTYGKSTDLSSSSTAGSN
                     FNNSIVGPISQFPDVMKGRSDFDVRHNLVLNAVYTLPSLGAGSGIAKALTDGWQISGI
                     YKIQSGLPFSAAIGGDSVGMLGANSFNFADRLTTPGCESAVNPGDPNHYIKTECFAFP
                     APGRMGNSGRNSLEGPGIKTLDASLVKMTRIKGASLQVRLEMFNVLNRANFAVPNRTV
                     SQIFNAAGRLNANAGKLISTSTTARQMQLGAKLSW"
     gene            14114..>14887
                     /locus_tag="PLT35_01550"
     CDS             14114..>14887
                     /locus_tag="PLT35_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27937.1"
                     /translation="MSSTHRKPSAYWNRILCSWVVLASCLMAGKALAQPANVLVTLPA
                     GAEAPSDLAATLAAWRQSGEVSDVLWLDAAQKEKAGFTTLILLEFATQGSYDVWAREE
                     APKLRAPLVVRRADVLTHGEITPRDSNRSIFRVEIVEPAGSAADYQKFVEGYVAPGWQ
                     EQRAQRILIRYTAYLERAVSGRRAQAVLVTEYRDAVAFERRHPIQEAFQAKRLASDPA
                     WKAFHEARAAASKTVSGTLASYIELPPPQLPGLPPYVPES"
BASE COUNT         2358 a         5052 c         5053 g         2414 t
ORIGIN      
        1 tcggaacgca gcgccgctcg tggccgaacg tcatcgaggc gatcaaggag ctgcgggccg
       61 ggatcatcgg gcggccgtac ttcgcgaagg gttggtatac caacaaccgg aagtcgattg
      121 gggtcggcgc ggagacgccg gttcccgact ggctcgactt cgggctgtgg caggggccgg
      181 ccccgaggcg gccgttccgc gacaacctgc tgcactacaa ctggcactgg ttctggcact
      241 gggggaccgg cgaggcgctc aacaacggca cgcacatgct cgacctgatg cggctgggtc
      301 tcggcgtcga gttcccgacg cgcgtcgcgt cgtccggggg gcgttttcat ttcaccgacg
      361 actgggagac gcccgacacg caggtgattg tcctcgacta cgccgagaag gtgtcgatgg
      421 cgtgggaagg gcgcagctgc tcgggccgca agatcgacgg cagcggcgcg ggggccctct
      481 tccacggcga gaacggcagc ctgctcatcg acggcaacac ctatctggtg caggacctcg
      541 acgagaaggt cgtccgcgag gtgaagagcg gcgccgacgt ggatccgcgg aaccccgcca
      601 acccggccga cagcctcgac gtcgcgcacg tccggaactt cctcgacgcg atccgcggca
      661 cggcgccgct ggcagccgag atccgcgaag ggcacgtgag cacgctgctg tgccagctcg
      721 ggaacatcgc ctaccgcacc gggcgcagcc tcgtcatcga caacaagacc ggccgcatcg
      781 tgggcgatcc cgaggcggcg ggctactggg cgcgggagta cgagaaaggc tgggagatgt
      841 cgctctgacg ggcgcgcgcg gccgccaaca ggggacggac gacggagggc ttgcgatgcg
      901 gacgacgacc gtgagacggg ttcttctcgt gggcgccctg ctgtgcggcg cggccgccga
      961 cgcgcagccg gccgatctcc gcatcgggat catcggcctc gacacctcgc acgtcatcgc
     1021 cttcacgaag tccctgaacg acgcctcgag cccggaccac gtcccgggcg gccgcgtggt
     1081 ggccgccttc aagggcggca gcccggacgt tccggcgagc gcgacccgcg tggacaagtt
     1141 caccgccgag ctgcagtcca cctacggcgt ccggatcgtc gggaccattg ccgagctctg
     1201 cgatctcgtg gacgcggtcc tgctcgagag cgtggacggg cgggttcacc tggaacaggc
     1261 gcgggaggtg ctgcggcgcc gcaagcccct cttcatcgac aagccgatgg cggccggctt
     1321 cgccgacgcg agcgaaatcg cacggctcgc gaacgaggcg gggaccccgt ggttcagttc
     1381 ctcgtcgctc aggttcgcgc ccgcctacca gcagttcctc gctgatcccg cccgcggccg
     1441 cgtgctgggc gtcgaggcgc acggcccagc ctcgctcgag ccgacgaacc ccggcttgtt
     1501 ctggtacggc atccacgccg tcgaaacgct ctacaccctc atgggtcccg ggtgcgtctc
     1561 ggtcgcgatg acgtcgaacg ccgactacga cctggcggtg ggcctgtggg aggacggacg
     1621 catcggcacg gtgaaggggc tgcggaaggg ccaccaggcg tacggcgcgc ttgtctacgg
     1681 cgagaaggcc gtgacctacc tcgcggtgac ggacgtgtcg tacgtgccgc tggtgcgcca
     1741 gatcatggcg ttcttccaga cgggcgcgcc gccggtcccg ccggcggaga cgctcgagat
     1801 catggcgttc atggatgcgg ccgagcgcag ccgcctcggg ggcggagcgc cagttcagct
     1861 gcggcgctag cagccccgaa tcagcctgct cccggagcgg ccgcgaggct cgtcagcgat
     1921 ccgggacgcc gtcgatcgcc gattccaccg gccagccgcc gcgcctatcg cccgcccgcc
     1981 gcgcggtcgc aaagcagctc gccgccgacg ctcacgttct ccggccggtt ctccctgata
     2041 atcaccacgt aggccggcct ggggtaaccg tacgccttct ccatggcgtc ggtgatctcc
     2101 ctggccagcg ccctcttcac acccagatcc tcgatgggcg ggccgtcgac atgaacgttg
     2161 ggcatctcgg ctcctttcca ggatgtctcg caggagtcta ccagccgaac ggcgcggtgt
     2221 cctcgtgttc ccgcgcgacc catgaaaacc ccgccgcgtc ctgcccgggc atcaggcaca
     2281 ctcaccgacg gtcgcgctcg accgatcgag ggagcgctgc gatgcacaga cggcagtccc
     2341 tcacccgggc tctcgccgcc ctggctggca gccgactggc cgcccgcgct tgagacgccg
     2401 gggggaaggc cgacctggtc ctgctcggcg ggaaggtcgt caccgtggac cggtcctctt
     2461 ccgtggccga ggccgtggcg gtgcagggcg gcaggatcct ggcgcgtggg catccaggcg
     2521 gccgtgaccc gcagggcagg ccgcggctcg ggcatgttcg gccgcggttc gatcagtccc
     2581 agcccccggg actcgatcag gcggtgtacg tccgggaggc catccggatg tgccccatcg
     2641 aaggcgcctg gctcgagcgc aaggagggcc tgaagggctc catcgagaag ggcaagctcg
     2701 cggacgccgg gaacgtttcg aagataggat aagcccccat gagaaaggca accgcaccgg
     2761 tagccgcgct ggccctctcc gccgcgttcg ccgtcgcctg cgccggccgg ccgccccggt
     2821 ccgtggcgca ctgggacgaa gacctgctct cgccgcagcg gtatcgggtc aggatcgaga
     2881 aggacgtcca ggtgccgatg cgcgacggcg tcacgctggc gaccgatctt tacatcccgg
     2941 aggcgcccgg ccggttcccg gccctgctct ggcgcaactg ctacggcaag gacgggtacg
     3001 gcgacatgag ctggtacgcc tcgcgcggct acgtggtcgt acaccaggac gtgcggggca
     3061 agcacgcctc gggcggcgac ttcgtccatt tccgcgacga cgccaacgac ggcttcgaca
     3121 ccgacgagtg gatcggcgcg cagccctgga gcaacggcaa gatcggcctg atgggcggct
     3181 cctacctggg ctacacgggc atcgcccagg cggtgcgcgg cagccgccac ctggccgcga
     3241 tgtccatctc ggtcgcgacc ggcgacgtct acaacggctg gatctacagc gacggcgccc
     3301 tgttcctcgg cttcgccttc ccatggggcg cggtcggcat gaacggccgg gaattccggt
     3361 ttcagaaccc cgtccccgtt ccctggcccg agatcttcgc ccacctgccc cttgagacca
     3421 tggacgtcaa ggccgggtat cccaacccgg tctaccgcga gtggctgcgg catccgcggg
     3481 ccggcgatcc ctattggaag gacgtgagct acgagaagga catcgccagg atctcgatcc
     3541 cggttctggt cctggagggc tggtacgacc tgttcctgcg cggcgcgctg caggaccacg
     3601 tcctgatcgc gagggacagc ctttccgcgc gggcccggcg acacaagcac ctggtgatcg
     3661 gcccctgggc gcacgagggc ggggtgcgca acaacgcgcc caaggccccg gccgccgggc
     3721 ccgatcgcag cctcgatttc ggcccggagg cggcggtcga gacgcgcaag atctatctgc
     3781 gttggcagga ccactggctc aagggcatcg acaacggcat cgaccgggag ccgccggtca
     3841 ggatcttcgt catgggcgag aacacctggc gcttcgagaa cgagtggccc ctggcccgga
     3901 cgcgctacac gaagtactac ttcggcggca ccagggccaa cagcatcaac ggcgacggca
     3961 cgctctcgac cgccgcgccg gcggcggaac aggcggccga ttcgtacgtc tatgacccgg
     4021 cctcgcccgt gcccaccctg ggcggcaaca cctgctgcag ccaggtgccg tcgggaccgt
     4081 ggaaccagat tgaggcggag atgcgcgacg acgtgctggt ctactccacg ccggagttga
     4141 agacgcccct cgaggtcacc ggcccggtca aggtcacgct ctacgcctcg agcagcgcca
     4201 gggacaccga ctggacggcc aagctcgtcg acgtgcatcc cgacggctac gcccagaacc
     4261 tgtgcgacgg gatcatccgg gcccgctatc gcgccggcag gacggccccg gccgatctac
     4321 tggagccggg ccggatctac gaatacgaga tcgatctgtg ggcgaccagc aacgtcttcc
     4381 tgccggggca cagaatccgg gtggagatct cgagcagcaa cttcccgcgc ttcgaccgca
     4441 acctcaacac cggcgaggac ccgatgacgg gcacgcgcat ggccaaggcc gagcagacga
     4501 tctaccgctc gaaacagcgt ccgtcacaca tcgtcctgcc gatcatcccg cgccggagat
     4561 agcgccgcaa ggccgggccg ccgctcgctc acggtggctg ccggaccttc ttgcgcagcc
     4621 gccgcggccg tcagggcagc cggcgcagct tctcggctac gggcaccctg ggctggggcg
     4681 gcggcacgtt ctccgggacg cccatggcca cgattccggc gatcgtgctg tccggtccta
     4741 cgccaaggag ctcgcggacc agcgggttgc tgccgatcga gagccagatg gcgccgagct
     4801 gcttctccca ggccgccaag gtgaggttct gcagcgccgc ggccgcgccg agcgggaaat
     4861 cgtaccggtc caggtccgtc gtcgccggcg ggcagacgac cgcgaagagc agcggggcgc
     4921 cgccgaagtc cacggcgaaa gcacgggcgg cctgtttgcg cgcctccggc agttcggggc
     4981 tgcgcagcaa cccggcttcc aggatctctc ggaagcgctc cgcgaggccc gcccgcgtgg
     5041 ccggcccgat gagcacgaat tcccacggct gcttgttgcc gtgcgacggc gcccaggtgc
     5101 ctgcctcgag gaccgccatc accgtctctt cagggaccgg gtcgggcctg aagctgcgga
     5161 ccgtccttcg ttcgcgaatc gcgtcgagca gttccacggt tcgtcccccc tcaccttccg
     5221 gccgtcagcg cggccgcgta ccgggatgcg gcgtacagat cgagacgagc gtagatcccg
     5281 aacgccgagc cgtcgtccag gaagccgccg cccggaccca gcgtgcgcgt caggataccg
     5341 ttcgcctccc ccagcgagag cgagggaaag gcggcggtca gcaggtggcc ggcctccggc
     5401 gcgacccggc ccacgtccgg gcgggcaatc gcctgctccg ggtggaccac cggcaacccg
     5461 tacgtctgcg tggacgcata gaacgcggcg ttggcggccg aatcgccgaa gcgcccgtga
     5521 tcctctcgcg cacactcggc gacggccttc ccgcaggcgg cttccagctg cgctctcacg
     5581 tcagcccgag ccgcgttcac cgcggcccgg aagtccgtga tggtggaggc tttctcgaag
     5641 gaccgcccga cgtagcgcgg atcgttggcc agcaggtgcg ccagcgcgtg gagcgccacc
     5701 gcgcgcccgc cgaggacgtc catggcgtag tgcgcgccga tggtgatgcg gtggttcccg
     5761 tactcggccg cgcgcgtcac catctccgga tatcgctccg gcaccatgat cgnnnnnnnn
     5821 nngagcaccg aacccacgta tccgtacgtc gtgtggccgc tcgggtagga cgggctgttg
     5881 accagatcca tcaccggccc gcggttgagc accgtgttgt ccgtgggcac gccgaaatag
     5941 tcccgcccga cgatgcgcgt gaagaccggc tcggtctgga acggccgggc gtctccgtag
     6001 gcgcccccgc cggcgctgcc ggcaggaaca cggtacgccc ggccgaagat gtccggcaac
     6061 ccgccgatct gctcgaggat tgcccgcgcc tccgtcgaca cgggcgcctt gccgtcggtt
     6121 gtcaggttcg cgaagaagta cttgcccgtg tccgaatcct cgcgggccgc cgagagcgca
     6181 taggcgatca cgccggcgac ggccggggac acgttcgtga agcgatccct gtcgatgtag
     6241 tgggcgcgag cctcgtaggc cgaccccagg gtggttccga gcccgtccgc caactgggtg
     6301 aggttgccgt gggtggagaa ggcgtctcga agcgccagtt gctggtcctc ggcgaacggc
     6361 agcagcgtcg gctgccgcca gtcaccggtc tggatgccgc cagtcaccgt gtagttcgcc
     6421 ccaagggccg ccagcccggc tctcgtcctg gtcaacgcgg tcaccggtgc gagccccttc
     6481 agcaccggca tggtggcggc cgactggccc gccgcctgcg acggcagcag taccgcgagc
     6541 gccagggcgc ctaggagtat cctgcgtgac atacccttcc ctctggttcc gatcgacggc
     6601 tccggacgcc gcccgccccg gttgtctgcc ggcacaggac gcgcagcacc cgactcaagc
     6661 tatcttatcg cgggcgaaag ccttgccgga gaacagccat gacacgatgg cctgtactcg
     6721 tcctcgttgc gatcgggatc ggcgccgacg ggtacctgca catgttggtc gccgcgcggc
     6781 tgcggcatgt ggccgcgacg cgctggggat cgcggcgcta ccttgccgcg gagtggctgt
     6841 tgacggacga cgcccgtgcc aagattgcgt gatctccagg caggggttac cgctgaacct
     6901 gcgaaagatc gttggcacta cccgccgaag cggcaacagc aggaaccggc cgcgaagcac
     6961 cgccggatga ttgatgctgg ccaacaggct gatgcgggtc agccgccttg tcgatcccgg
     7021 gcggatcagc cccggtccga atcggcctcg ggcctggtat tcagactggg acggctcact
     7081 cgagcaggcc aagctggctg cgaaccacgc tggcggtcag tggcaggtag tccccggccc
     7141 gctcgacgtc ccgctgaccg tcgcggctca gaatgtaccg caggaactcc ctgattgcgg
     7201 ggtcgagcgg ccgtcccggc ggccgattga ggaagatcca cgtggcgcgg tgcaaggggt
     7261 atcgtcgggc cgccaggttc tccggcgtcg gggcgtagaa gggcccttcg ttgcctgccg
     7321 agagggcaag cggcttcacc gcggcgccgg cataggccag ggccgacaac ccgatccctc
     7381 cgggctcgcc ggccagggcg tccaggacgc gttgacgccc atcgtgggaa gacccgtcgg
     7441 gccggcggac gtcgtggaac tccgtcatgt cgcagttcca cttgtagctg cccttcaaga
     7501 cagcctccct gaagaacgat ccggtcgcgg tctggatgtc gggcgcatag acccggatcg
     7561 gcctcgccgc ccagctgcca ccgaggccga gatcgcccca tgtccgcgcg ttggcctggc
     7621 cccgccggtg ttcggatccg aacacagcgt cgagttgagc gagcgtcagc tgtgagatcg
     7681 ggttgtcctt gtggacgaag acgacgggcg cgaacgactt cccgggcact ccgaggctcc
     7741 ccgtgctgac ctgaaccccg agcggcctgt agcggaacac ccactcgaag gccatggtct
     7801 cggttgaatc cgcgggccgg cccatcagcg cgatgtccgc gacgccggtg tacaggccgg
     7861 caatgcccgt accggtgccc attagagagg tttcgaacct cgcggccggg tggtgtcgtt
     7921 tgaatccggc ctcccacagg gccatgagcg gcttcatcct gtcgttgccc caggcccgta
     7981 tcgtccccga cacgcgcacc gtcggctcgt acgcctgcag gtcctcccgt tgcgcttcag
     8041 gggcggcagt gacggcgagg agcgtgatga caagcagcgc ggacagtggt tgggacatcg
     8101 cggctcagcg gttcgagttc cggtatccag tcggcggact actgcagtct cagggtctgg
     8161 tctcgcacgg cctcggtgct gagcggcagg taccggccgt cccgaacgat cgcgtcctgg
     8221 ccctccctgc tcaggatgta gcgcaggaac tcgtcgacct tcgggtcaac ggccgcgccg
     8281 ggcgggcggt caacgaacgc agtgatgaac cgggtcagcg ggtaccgccg gctgatgaga
     8341 ttctccttgg tggcgtgata ggccgggccc gcggggtcag tcgccagggc cagcggcttc
     8401 acgagcggcg tcgcatagag caggctcgag accgctatcc cgtaccggtc ctgcccgagg
     8461 ctgtcgagaa cctgctggcc gctgtcgagc agcgtgccgt cagggcgctt cacatcgacg
     8521 tactccctca atcccgggtt ccagcgccca cttcccccca tcaccgtgtc ctggaagaac
     8581 agcgcgaatc ccctgctgag cgcgaagccg tacgtctgga tgggtcggcc ggcccactcg
     8641 cccgccagac ccaactgacc ccaggttcgg atattggcgg acccgcggcg atgctcgcac
     8701 ccgaacaccc cgtcgagatc cgcaagcgtg agctggacga gcgggttgtc cttgtggacg
     8761 aagaccacga gggcgaagtc gaagttgcgg acgtcgaggc tcccggtcgc aatgtcgatt
     8821 cccagaggcc ggtacccttt gacgcgttcg aatgccgcga gttccgaccg gaggatctcc
     8881 cgcccgagga ttgccacgtc gcccacgccg gcatagagcg caccgattgc cgaggcggtg
     8941 ccgtgcatcc ggtcgtcgaa gcggatgcct ggctgatggc gctggaaccc cgtctgccac
     9001 gcgctgacga gttcgcccat gaagtggttc gtcggacttc cgtgccccca gagccggatt
     9061 gtcccgctga cctgcctcgc cggccggtat gggcgtatgg gctccgtttg cgcggcgagc
     9121 agaacggtgg cgctggcgat cccgaggatc gccagcaccc acgcgggcca ccgagggagg
     9181 gagatgaccc gttgcgacga cacggccggg cgagctgccg acggcggtga ctgaggcgtc
     9241 aaggggcctc cgtccaaggc cagcggtgac cagctagtcg agtttcgcca gctgttctcg
     9301 cgcgaccttg gctgtgagag gaaggtactt gccgtccttc atgatcagtt cctgcccttc
     9361 ccggctcagc acgaaccgca ggaactccct gactttcgaa tccagcccct ttcccgggag
     9421 cgcgtgagtg tagaagtaca cctcgtcgaa cagcgggtac gtccggttct gcagcgtctc
     9481 gatcgtgtac tccacgtacg ggccggccgc cgtctcggcg agcggcagga tcttgagtcg
     9541 cggtgacacc tgctcctgcg ccgcgaagtt catggtgacc gtgggcgcgg cgatgtaggc
     9601 gatgccgtac ttgtctttca ggagatcttc gttcagtccg cggctgagcc tgccatcagg
     9661 cgtgacgtag ttcgcataga tccgcagccg ctcattccac ttgtcgctgc ctttcagaac
     9721 gcggtcggat atgacggtgg cctggtggta tcggagattc agcccatagg gaatgatcgg
     9781 cttgtcggcc cactcgccgg tcaggccgag ctggccccac gagcggatgt ttctctcggg
     9841 cccgcgggcg aactcgggat gccagtccgt cccgacccag ccgccgagcc gctccgcgcc
     9901 gaagattccg tcgagctgct cgagggtgat ccgggcgagg gggttctccc ggtggacgac
     9961 gatgccgtac cccgggctcc agccggtcac gtcgtaggaa cccgtcgcgt atacgatctc
    10021 gaacggatca gcattgaggt agcgttggta gaactggagg ttggggaacg tgatcttcac
    10081 gctcggaccg agatccgact gcccgctgac cagcccaggg atggtgcccg aggtggcctt
    10141 cagcctgaag tcgaaggcga tctccgggtg gaacttgcgg aaggcctcct cccagtatcc
    10201 ggcgagcatg ccgtcggccg gatagttgct gccccacatc ctcagggtgc cagacacccg
    10261 ctgctggggc ttgtacgccg gaatgccgct caggtcgaac gtccgcgtgt agtaggcctt
    10321 cttgcctttc gtcttcagcg cttcagcgcg ggcctcctgc atggccatgc ccgcggtact
    10381 cccgccctgc tgctgctccg tggcgggcgt tgacgggctg tgcccggaag ctgtggagac
    10441 gagcacgagg gcgacgccag cagccagcaa cagcccgacg tggaggtagt gtctcaccgt
    10501 cgcttcccct tcctcttctt cctcatcccg gcgccgcccg cggggtttgg cgcaggagcg
    10561 gccctgcgcc tcccgccggg ctttcagccc gcgcgccgtc accagctcaa cttcgctccc
    10621 agctgcatct gccgggccgt ggtcgaggtc gatatcagct tgcccgcgtt ggcgttcagc
    10681 ctcccggcgg cattgaagat ctgcgacacc gtccggttcg ggaccgcgaa gttggcgcgg
    10741 ttgaggacgt tgaacatctc gagccggacc tggagcgagg cgcccttgat tctggtcatc
    10801 ttgacgagcg atgcgtcgag cgtcttgatg ccgggcccct ccaggctgtt gcggcccgag
    10861 ttgcccatgc gcccaggggc tggaaaggcg aaacactcgg tcttgatgta gtgattggga
    10921 tcgcccgggt tcaccgccga ctcgcagccg ggggtcgtga ggcggtcggc gaagttgaac
    10981 gagttggcgc cgagcatccc caccgagtcc cccccgatgg ccgcagagaa cggcagcccg
    11041 ctctggatct tgtagatccc ggagatctgc cagccgtcag tcagggcctt cgcgatcccg
    11101 cttccggccc caagggacgg cagcgtgtac accgcattga gaacgagatt gtgcctcacg
    11161 tcgaagtcgg aacggccttt catgacgtcg gggaactgcg agatcggccc gacgatcgag
    11221 ttgttgaagt tgctgccggc cgtggacgag gaactgaggt cggtgctctt gccgtacgta
    11281 taggacaccc ccgcccggag gcccccggcc tgccgcctca agcccacgtt gagggcgttg
    11341 tagtcgttct tgccaatcca caggagggtg ttgatgaccc cgacattggg attcagcttc
    11401 ttgccgctct tgagcggcag cggccattgg agcgagccgt ccggcagccg ctcgggaacg
    11461 acgatgttgg agtcattcgt gcggaacggc tggtggacac catgctggcc gacgtagccg
    11521 acgtgcacga cgaagctcgc cgggagctgc cgttcgacgt tgacgttcca ctgcgtgacg
    11581 taggagcggt caggatcctt ctggatgtac gccgcgcgag tgtcgttcgc cgtgagcatg
    11641 ccgtacgccg ccgtcgggaa cgtgtcggcc ttgagccccg tgagcgtgcc ggtccggaag
    11701 aagggctggg tgttcacgat caacagcgag agctgataga ggagcggcag ggtgtcatag
    11761 acgccgacgc cgccccggat ggcggtcttc ccgttgctga acggatccca ggcgaacccc
    11821 aggcgaggcg agatgttcca cttcgtcggg ttgttgaagt acggagaccc gatgcgcggc
    11881 gccgggtcgg tgatgttgtc caggttgctg aaacggtctt ccttctcggt cggcagcccg
    11941 accggctcgt agcggagccc caggttcagc gtcaggttcg gcctcacccg gaagtcgtcc
    12001 gcgacgtaga cgccgaagac gttctgcgcg aggtacatcg tcgggttctt gccctgaatc
    12061 gaagcggtga acgacgtggg ctggttcgtg agaaagctcc gaagcgagcc gaacacccac
    12121 cgcccgttcg gggcgttgga cgtcgtggtg tcgtagtagt tgcgctcgaa ggccacgccg
    12181 actttgatga cgtgaacgcc cttggtccag atcaggtcgt tgtagatctg gaggctcttg
    12241 tacgagtgga acgattcacc gacgccgccc ttgccgccgt ccaccccggt caagccgctg
    12301 acttcgatgg tgcccatcgg attgccgggg acagacccca gcgacaggtc gtcgaacagc
    12361 gggttgaggt tctcgatcca ctccggcgcg tgagattccg agctgctgaa gccgactctg
    12421 gagacgctga cgaggttcga gctgaagatg cgcgtgtatt cgagcgagcc catggcccgg
    12481 ttcgacgtcg ctcccacgat gacgccgccg gattcgttcg ggccctcccg atgtgagcgg
    12541 tccaccagga agctgccgtg cagcatggcg ctcgcagacg ggttgtagtc gacgcgcgcc
    12601 gtgtacagat cgtccctgga ctgcgagttg gagacgaacg agtaggtgcc gacgtcgccg
    12661 gattcaccgc ggttcggcag ggggtagatg cccaggtacg gggcgacgag cgggctgacg
    12721 gtcaccgcgc cggactccag cctgccctcg cgagcggcga ccgacggcac ggtgatcaag
    12781 tcggtcgtgc cgaggtcctg tcgaaggcct tcgaagtccg cgaagaagaa cagcttgttc
    12841 ttcacgaccg ggccaccgag cgccgcgccg tactggttcc ggtagaagtc cggcttctca
    12901 ccgtcgtcga agtagttcgg ggcgtcgaag gcgtcattgc ggaagaaccc gtacgccgac
    12961 ccgtggaagt cgttcgatcc ggaccgagtg acggcgttga tgacgccgcc cgatgtcttc
    13021 ccgtagtcgg ccgacgcatt tcccgtgacc accgagaact cgccgatggc atccacgccg
    13081 agaacggacc ccagcacgcc gcccggtccc ccgccggagt agtcgttgat gctgatcccg
    13141 tcgaggcggt agttgttctg ctgcggccgg ttgccgccga aggacatctg ggttccccag
    13201 ccgcggttcg cccgggcgtt gccgcctccc gagagatcgg cctgcgcctc gatggcgtgc
    13261 acgccgggtt cgagcgtcgc catcatggtc cagtcgcgtc cgttcagagg cagttcacgc
    13321 accacgtcgc ccttcacgac ggtgctcagc gtcgaggacg agagggcgat gtcggcggtc
    13381 gcggcgacca cgtccacttt ctcctcgacg gaggaaatct gcatgcggat gttcgcgacg
    13441 accgtgcttc cgacatcgac cgacacgccc tcttgcaccg ccgtggcgaa ccccgtggcc
    13501 gccaccgtca ggtcgtagcg cccgcttgcc aggttcggca cgctgaagaa gccccggtcg
    13561 ttggtcagga tcgtgcgcac ggcgccggtg tcctggtgca ccaggatgac gtccgccttc
    13621 gggatggcgg cgccgcttgg atcggtgatc gtcccgttga ccgtgccgcc cgagccctgt
    13681 gccagcaccg gaacgctgaa gcacatcgcg cagcacacga gggccaagac ggccgcgatc
    13741 ttcgcgcgcc gcgcgccggt cgccagtccc gtctcccagc tccgcctcgt actcatgaca
    13801 cctcccaaga ccccagcgta gtcgagactg cagcagtagt ggagggcacg gcccgcaagg
    13861 cacgcggaac gtgatcccgg cgcgccgggt tactcgccca tcgggcccat aggcgactta
    13921 tgccgcaaca gcggagccgc ctcctactga agagttgctg aattcttttt aagggccccc
    13981 gcgcgtcgaa cgcgtgcggc ggactgaacg tttctttacc aacacttaag gtacgcccgc
    14041 tacgcggagc ccggtatcat ggccccacgt cggcagcagc ggcgttctct gtcagtggaa
    14101 gaaaggggag cgaatgagca gcactcaccg gaagccgtcg gcctactgga accggatcct
    14161 gtgcagctgg gtcgtcctgg cctcgtgcct gatggccggc aaggcactcg cgcagcccgc
    14221 caacgtcctc gtcacgctgc cagccggcgc cgaagccccg tccgatctgg ccgcaacact
    14281 tgcggcctgg cgccaatcgg gcgaggtctc cgacgtgctc tggctcgacg ccgcgcagaa
    14341 ggagaaggcc gggttcacga cgctgatcct gctggagttc gccacccagg gatcgtacga
    14401 cgtgtgggcg cgcgaggaag ccccgaagct gcgcgccccg ctcgtcgtca ggcgggccga
    14461 tgtcctgacc catggcgaga tcacgccgcg cgactccaat cgatcgatct tcagggtgga
    14521 gatcgtcgag cctgcgggat ccgctgctga ttaccagaag ttcgtcgagg gctatgtcgc
    14581 gccgggctgg caggaacagc gggcacagcg gatcctcatc cgctacaccg cgtacctgga
    14641 gcgggccgtc tcggggcgaa gggcccaggc cgtgctggtg acggagtacc gcgatgccgt
    14701 cgccttcgag aggcgccatc cgatccagga ggcatttcag gcgaagcgcc ttgccagcga
    14761 cccggcctgg aaggccttcc acgaggccag agccgcggcg tcgaagacgg tgtccggcac
    14821 tctggcctcg tacatcgagt tgccgccgcc ccagctgccc ggcctgccgc cgtacgtccc
    14881 cgagtcc
//
LOCUS       DAOSIF010000020        14695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4210_length_14695_cov_4.870082, whole genome shotgun sequence.
ACCESSION   DAOSIF010000020 DAOSIF010000000
VERSION     DAOSIF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14695
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4210_length_14695_cov_4.870082"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLT35_01555"
     CDS             <1..739
                     /locus_tag="PLT35_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOG27938.1"
                     /translation="WLNLFWQHPMKTECADKYTLRDYVTRQGLHRLLPTLFAVYLSAD
                     EIDFAQLPDRFVLKCSHGCKCNVFCFDKRDFDTAVAQRRLRRWMDTDYSLLYGELHYA
                     RMKPRILCEELLNDHTARFPRDYKVYCFNGRPLYIRCCYDRDANGKSRSVVVDVDWNP
                     VVVDRNQPVENDPISPPQTMPQMLEAAMDLAAPFPFVRIDFYSIRDRLVVGEMTFTPD
                     ACINRDYTADAQREMGRLILLPSVYAR"
     gene            870..1838
                     /locus_tag="PLT35_01560"
     CDS             870..1838
                     /locus_tag="PLT35_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOG27939.1"
                     /translation="MIRSSSSQSVGAVPVATRARLAQYRRLVSAWHIARGCAFSILAR
                     ASPELLGRLRFRAAWGRWPDFENPTTFDEKLLWLNLYWRHPLKTECADKYAVRGYADR
                     LGLGALLPRLYGVYDSAQAIDFGSLPERYVLKCSHGCKCNLFCLGENGVDVAVARRSL
                     ERWLRTDYSHMLGELHYRNMKPRIICEEYLDEGNGRLPTDYKVFCFGGRPTWILCYSG
                     RDPNGKGQRSLVDIDWQPSAVLRSPDAQAPPPRLRTLPEILTAAEVLASPFPFVRIDF
                     YSIGKRVVLGELTFTPNGCINVDYDQLEMGGRLLLPPPYPGHGASA"
     gene            2336..3031
                     /locus_tag="PLT35_01565"
     CDS             2336..3031
                     /locus_tag="PLT35_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27940.1"
                     /translation="MYPGPFPSFLWTLAGFDSSGLIQDYISGILLEPRLSPVFEAPHG
                     VMSCGLVGIDLLPSRGSLYFPESNFTPGHSPQRDRISPADDTVCNYLAAWASGQGFTK
                     LVFFSYNYPAVFEQSMEEAWQTVGKRNGIRIEIVDDPAFASPWRRSRRVLMDPHARGT
                     LYVNGRHLPGPLPRLVSRKGLMEAAIRRFWGPSITTGCLALPGLVRRDADLRPLDEQS
                     PFPNLIIKRADLD"
     gene            3125..3430
                     /locus_tag="PLT35_01570"
     CDS             3125..3430
                     /locus_tag="PLT35_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27941.1"
                     /translation="MATGAQGTGLGCAHIFRAYLLVTPRGPVYLGARKDVSSVPIPAS
                     LPLGEVQDKSPYVVNVRLGAYSVPPSEAEDQRCREAVLAVGRAVHEFPQEKHVLTVL"
     gene            complement(3494..4084)
                     /locus_tag="PLT35_01575"
     CDS             complement(3494..4084)
                     /locus_tag="PLT35_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27942.1"
                     /translation="MRFVRFFTLKVLRRTIGFEVLCLYRGDVESGPHAAADGNIGVRT
                     ASNDDLREIESLRSERPSGWVKDRISLGESCFVARTRGGDLCGYLWAAPGPRIFRHRG
                     KEIRIAQGDVYVRDGQTLPAYRGRGIMPQLIASARATLLGQEKRWMWAAIYDFNDSPK
                     RSVEKAGFQLHEKICYVSVPFAGRALFCSRKQKPAV"
     gene            complement(4181..4969)
                     /locus_tag="PLT35_01580"
     CDS             complement(4181..4969)
                     /locus_tag="PLT35_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27943.1"
                     /translation="MNTRHVDDQPVALLWSGGWDSTYRLLHLAVVESRPVQPIYALDH
                     LRQSSLHELAAMLAVRDAVAARHPAAVGLLRPTQLYLVSEIPVLEEVTARYRSLSSRF
                     RLGGQFEWLPRLAATSGMTGLELAIERNEYRPRDPVFAYLGRWLVERGGALELDERVP
                     DADLLLFRHFRFPLYHQTKRQMLDASRRHGFCDILLRSWFCHRPRRGKPCGTCHPCID
                     AMEEGFAFRVPAASRARYHAYAAKRALKRLLRRAGAARLPRMGN"
     gene            5497..6822
                     /locus_tag="PLT35_01585"
     CDS             5497..6822
                     /locus_tag="PLT35_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG27944.1"
                     /translation="MASTLRGVLARARASEFLRNILLVMAGSGAAQAISIALVPVISR
                     LFTPSDFGVSGSFAAVSGVIAAAVTLEYSQAIMLPREKTDAIGLLAVSLASTGVVTLL
                     TLTACVLAPSLVNGLIKTQGALVLVLLVSATLISGTNTALQAWAVRAKAFRHTSASQV
                     VRSLSGKSATIGFGLLGTGAPGLIAGEILGNVAASINVLRVLLPDLGLLARQARRASM
                     VRLAKEYRDFPMYTASQGVINALSSGLPVLLLTRFFGLPVAGSYAFALSVLTFPMGLV
                     LSALRQVLFQKASESQHQGRSLSALYVRVTATLFAMALLPTAVILVWGPQLFSLVFGT
                     RWYTAGEFSRSLMIWMAVVFCNLPATLFGRIIRIQRFVFFYDLSLLALRTAALVLGGY
                     FLSASQTVMAYAVVGAAMNAYLILAVGRAVMKKELAMRPEGILSYLAGD"
     gene            complement(6761..7543)
                     /locus_tag="PLT35_01590"
     CDS             complement(6761..7543)
                     /locus_tag="PLT35_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27945.1"
                     /translation="MSEPGARRGWRRHFFYRSERTRTTWKLRLGALGLAAGLLWLGRG
                     WWTEAIARSLVCEPSLVASDAILIENLEPDYLLFERARQLRRAGMAPRVLVPITLDRN
                     GRELNDVAQGVAEVMVRISGVGDVEFVPMRQMEPITLNAARDVREFLENQRIRSVIVV
                     TPLFRSRRSALVYAATLGRGGIAVHCAPVQGSRGVKNWTASWHGIQEVVQQCVKLLYY
                     RLVVLPHDAVLRSPGRAGQSARASPPPGSSGSPPGAWQVPSS"
     gene            7542..8774
                     /locus_tag="PLT35_01595"
     CDS             7542..8774
                     /locus_tag="PLT35_01595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG27946.1"
                     /translation="MRRPPADEARGDAGAPRRTRIGAAGASIIARDGERAAPPAGAGA
                     APAPQPTPQPTPDPAPGGLVELGPADFARWDDFVERHPDGWICHLSDWLRTLEESFPH
                     ITGRVLASERDGALRAGIPVCEVRSRVLGTRLVSVPYGTLCCPLAPDDEVGRLMEGVL
                     DLGRRAGARCIEVRAVRPLSAAAAERFARQARYTHHSLDVTAGAEALRLRLHRSCVRQ
                     RIDRALARGVVDEEAQDEGGLRAFYDLYVRCRKRLGLPPQPYRFIRSMWLAFRPQGRL
                     EVRLASCRGTPIAGILLLRFNGRTSAEYLVSDDGRRHESPGHLLVWNAIRRSHELGCR
                     VFDFGRTDAANEGLMAFKNRWGAQVTGLPVYYHPKSRLQAVESRRAALRSAGRVVFRR
                     LPARANRWLGELIYRHAG"
     gene            8907..9773
                     /locus_tag="PLT35_01600"
     CDS             8907..9773
                     /locus_tag="PLT35_01600"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOG27947.1"
                     /translation="MSRWNGRPGRERQWLGGVALLTLLLALPLARLAIYAWDSSLHSH
                     VVLIPLVSAYLLYTRRGTLPAAGPASPAGAALPAAIGAAALAAAFAWRERLSVNDGHA
                     LVALAYASLVAAGGFLFLGSKWMRAAAFPFAFLVFMVPLPDALAAALERASAAASAEA
                     AALYFAAAGTPLVRQGTIFELQTITMEVAQECSGIRSSWVLFITSVLAAQLFLGSPWR
                     RLILVAFVIPLGILRNGFRVFVIGMLCVRVGPHMIDSLIHRQGGPLFFALSLAPLFLL
                     LWWLRRGERRRR"
     gene            9770..11887
                     /gene="prsK"
                     /locus_tag="PLT35_01605"
     CDS             9770..11887
                     /gene="prsK"
                     /locus_tag="PLT35_01605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HOG27948.1"
                     /translation="MTLFQFLPFAAAALSLLLAAAALVRKKPSAATWCFVAGMAIFSL
                     DSLLTGLSLREPALEAAAGWLTLSLLAKSFLPAAWLGFGLTYSRGDPRASLADARPVV
                     TALALLPVAIALAFRGSLLQAVPLAGTAGEVLVLRVGTAAKALNVALLLGFALALMNL
                     EQTFRSAVGTMRWRIKWVILGLAVVFGGHLYVRSQAILFPVYDPALAGVESSALLIGC
                     AFLVVAYARTGLAEADVYPSRAVLRSSLTVLIVGGYLVVVGVLAQLVRRFGGAESFQL
                     GAFVVLLGMAGLGVLLLSDRFRQRIQAFVARHFGRAQHDSVRVWTQLSQRLAKVNDEA
                     GLCEAAAKLACETFDVLSATVWLMDEAKGRLALGASTSARAVEAARAGPGAASAAAIA
                     AGLQTRTAPFDLEAVAAPWADECRRLNPSTFPTGGRRWCVPLHTGEQCLGALVLADRV
                     NGAPYSVEERELLACIGAQATSFLLNLRLAQEVARSRELEAFRTMSAFFVHDLKNTAA
                     SLNLTLKNLPVHFDNPAFREDALRAVGNTARRIEELIAKLSALRQRPELKPAPLDLNQ
                     LVGEAIAGAGAMPGVDVSADLGALPPVAADREQIRSVVTNLLLNARDAIGSAGGRITV
                     RTERLDGRALLTIADTGCGMSPEFVRDSLFRPFLSTKKHGLGIGMFQARMIVEAHGGT
                     IQVDSEPGRGTTFRVSLPAGHEP"
     gene            11884..13269
                     /gene="prsR"
                     /locus_tag="PLT35_01610"
     CDS             11884..13269
                     /gene="prsR"
                     /locus_tag="PLT35_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HOG27949.1"
                     /translation="MTKPALLIVDDDEDIRTQMRWALADEYEVAAAGDQAGALAAFSQ
                     HRPAATLLDLGLPPQPNGPDVGLATLSALIGLDHEAKVIVVSGQGDRQNALKAVGSGA
                     YDFLCKPVDMDELKLVLKRCLYVAGLERDFRALQEQARPESFDDMLGASPLMHAVFDF
                     IRKAATTGAPVLILGENGTGKEMVARALHRRGRQRSGPFVPINCNAIPENLLESELFG
                     HEKGSFTGAHATRKGLVESAAGGTIFLDEIGELPASLQVKLLRFLQEKTFQRVGGRQE
                     LQSDARVIAATNVNLQEAVGSGKFREDLYFRLAVLVVTMPPLRERGGDVGLLAREFLR
                     RFAAQHGRPGLTFALDAVRALNAHAWPGNIRELQNRIQRAAIMAEGKRVTAADLELAA
                     PGDARRTQTLREAREQVERQVVEDALRRHGGKITAAAAELGVSRPTLYELMEKLSISK
                     TEGGPAGVSRD"
     gene            13273..14265
                     /locus_tag="PLT35_01615"
     CDS             13273..14265
                     /locus_tag="PLT35_01615"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG27950.1"
                     /translation="MNVLVTGGAGFIGSHVAERLVARGRRVVVLDDLSGGFAENVPAG
                     AELVRGSITDATLVDRLFEANRFEHVFHLAAYAAEGLSHFIRRFNYTNNLTGSVTLVN
                     AAVNHGTRCFVFTSSIAVYGASPELPMTEATPPHPEDSYGIAKLAVEQDLAAARAMFG
                     LDFVVFRPHNVYGERQNIGDRYRNVVGIFMNQALAGRPMTVFGDGMQTRAFSYIDDVA
                     PIIAESIDVPGAYNQVFNIGAEQPCRVNDLAAAVARAMGVPPAVVHLPARQEVQDAYS
                     AHEKLARVFGTRPPCGLEEGLERMARWVRRQGARRGQRFEAIEVTRNLPAAWLD"
     gene            14269..>14695
                     /locus_tag="PLT35_01620"
     CDS             14269..>14695
                     /locus_tag="PLT35_01620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG27951.1"
                     /translation="MGPLPLVACVILNSNRRADTLECVGSLLAGSYSNLRVFVLDCQS
                     RDGSVEAVREAFPSVAVVELDRNLGYAGNNNVGIRLALGEGAGWVFVLNEDTVLAADC
                     LERMVQAIVVRPDVGIAGPMVYHHDEPDVIQSAGGGLTRF"
BASE COUNT         2223 a         5073 c         4971 g         2428 t
ORIGIN      
        1 ctggctgaac ctattctggc agcatcccat gaagaccgaa tgcgcagaca agtacactct
       61 ccgagactac gtcacgcgcc aggggcttca ccgtttgttg ccaaccctct tcgcggtgta
      121 cttgtcggca gacgagatcg acttcgccca gctacccgac cgcttcgtgt tgaagtgctc
      181 gcacggttgt aaatgcaacg tgttctgctt cgacaagagg gatttcgata ctgccgtggc
      241 gcagcgtagg ctacgacggt ggatggacac tgactactct ctcctgtacg gcgagttgca
      301 ttacgctcgg atgaagcctc gcatcctatg tgaagagttg ctcaatgatc acaccgcgcg
      361 gttccctcga gactacaagg tctattgctt caacggacgg ccgttgtaca ttcgatgctg
      421 ttacgatcgg gacgctaacg gcaagagccg gtctgtggtt gtggacgtcg actggaatcc
      481 agtagttgtg gatcgaaacc agcccgttga gaacgatccc atttctcccc ctcaaacgat
      541 gcctcagatg cttgaggcgg cgatggatct ggccgcgccg tttccatttg tgcggatcga
      601 cttctacagc attcgtgacc gactggtagt gggcgaaatg acgttcacac cggacgcgtg
      661 catcaacaga gactacacgg ccgacgcgca aagagaaatg gggcggctca ttctgttgcc
      721 gtctgtttac gcacgttgag caatgcgaca ctgaaagggg gcatccttgg ccctcccagg
      781 aggcgccgtc gtcggcggtt ctgtgcctcc tcacaccgtc acgccctcag gtcgattgac
      841 gtcggttact gaggccaccc gaaaagccat tgatccggtc ctcgtcgtcc cagtcggtag
      901 gtgcagtacc cgtcgccacc cgagcgaggc ttgcgcaata ccgccgatta gtctctgcgt
      961 ggcacatcgc gcgagggtgc gcattcagca ttctggctag ggcctcccct gaactgctgg
     1021 gccgactccg atttcgcgcc gcctgggggc ggtggccgga cttcgagaac ccgacgacct
     1081 tcgatgagaa gctcctctgg ctcaacctct actggcgcca tcccctgaag acggagtgtg
     1141 ccgacaagta cgcggtcaga ggatacgccg accggctcgg ccttggcgct ttgctgccgc
     1201 gtttgtatgg agtctacgat tccgctcaag ccatcgactt cgggtcgctg ccggaacggt
     1261 acgtcttgaa gtgctcacac ggctgcaagt gcaacctgtt ctgcttaggc gagaatggcg
     1321 tcgacgtagc tgttgcgcgg cggtcacttg agcggtggct tcgcacggac tactcccaca
     1381 tgcttggcga gctgcactat cggaacatga agccgcgcat catctgcgaa gagtacctgg
     1441 acgaaggcaa tgggcggctc ccgaccgact acaaggtgtt ctgcttcggc gggcggccga
     1501 cttggatcct ctgttacagc ggtcgcgacc ccaacggcaa gggacagcga agcctggtgg
     1561 acatcgattg gcagccctcc gccgttctgc gaagtccgga cgctcaggct ccgccacctc
     1621 ggctgcgaac tctaccggag atcctgactg ccgccgaggt gctggcgtcg ccatttccgt
     1681 ttgtgcggat cgacttctac tccatcggga agcgcgttgt cctcggcgag ttgactttca
     1741 ccccgaatgg ctgcatcaac gtagactacg accagctcga gatgggtggg cgcttgctgc
     1801 tgccgccacc gtacccggga catggtgcgt ctgcgtgaag atggcgcgct caaggcccct
     1861 gccgcgcgcg ggggcacggg cggaacgccc ggcatcgccg agcgcattgg accggctctt
     1921 ctgcagttga tcctgcaaca ggcagctcgc gacgtgacgc gtgggcaggc ggcagggact
     1981 atcgccgcgt ttctgggtgt tcaccaggcg aacctggcag acgcccgaga gacgctgtcg
     2041 catcctctcc gatcgtgggg cccctttgag gctctttcac gggaggtccg actaagcctc
     2101 gcggcggcag cgtccttcca cctccgggag gttgcaggcc tcacgaatcc gatcgcgggg
     2161 gtactcacgt acggcccgtc agcgcggtcg gcggggcgct gacgcacttg gccgcgaaac
     2221 acggtgacga gggaagcccg tcctccagta cactgcccac gcgggtggcc aagaggctcg
     2281 tcggcttggg ccgcaaggcc ggcctgactg caacgacggg gatagagcac accccttgta
     2341 cccagggccg tttcccagtt tcctctggac tctcgccggc ttcgactcgt cagggctgat
     2401 ccaggactac atcagtggaa tacttctgga acctcgcctg agcccggtgt tcgaggcgcc
     2461 tcacggcgtg atgagctgcg gcctcgtcgg aatcgacctg ctcccctctc gtggcagtct
     2521 gtatttcccc gagagcaact tcactccagg ccacagcccg cagcgggatc ggatctcgcc
     2581 tgccgacgac accgtgtgca actatctcgc agcgtgggcc tcaggccagg gcttcacgaa
     2641 gctcgtcttc ttctcctaca actatcccgc cgtcttcgag cagtcgatgg aggaagcgtg
     2701 gcagacggtc gggaagcgga atggaatccg catcgagatc gtggacgatc cggcattcgc
     2761 gtccccgtgg cgccgctccc gccgcgtcct gatggatccg cacgcgagag gaactctcta
     2821 cgtcaacggc aggcacctgc cgggcccgct gccgaggctg gtctcgcgga agggtctcat
     2881 ggaagcagcg attcggcggt tttgggggcc ctcgataacc acgggctgcc ttgcgctccc
     2941 gggtctcgtt cggcgcgacg cggaccttcg cccactcgac gagcagtcgc ccttccccaa
     3001 cctgatcatc aagcgcgccg accttgacta ggccaagggc gtttccctgt accgaacgcc
     3061 gcgacttcca gagcgcgcca atgcgtggtc gaacatcgct tacgaataca tccctcctga
     3121 tctcgtggcc accggggctc agggcactgg actcggctgc gcccacatct tccgcgctta
     3181 cctgctcgtc acgccacgag gtcccgtgta cctgggagcc aggaaagacg tctcgtcggt
     3241 tcctatcccg gcgtcgctac cgctcggcga agtgcaagac aagagcccct acgtggtcaa
     3301 cgtgcgcctg ggcgcctact cggtgccgcc ctcggaagca gaggatcagc gctgccgcga
     3361 ggcggtgctg gcggtcggca gggctgtgca cgaattcccc caggagaagc acgtcctcac
     3421 cgtgctgtag agactgcggg tcacaaggtc cctggctcgc ctgcggcaac cctcgctgcc
     3481 atgtcagcgc cgatcacaca gccggtttct gctttctcga gcagaacagg gccctgcccg
     3541 caaacgggac ggacacgtag cagatcttct catgcagttg aaaccccgcc ttctcaacgg
     3601 accgtttcgg agagtcgttg aagtcgtaga ttgccgccca catccatcgt ttctcctgac
     3661 cgagcagcgt ggctcgcgcg gacgcgatca actgcggcat gatgccacgg ccgcggtagg
     3721 caggaagcgt ctgaccgtcg cggacgtaga cgtcaccctg ggcgatcctg atctctttgc
     3781 cccggtggcg aaatatcctt gggccgggcg ccgcccacag gtatccgcac aggtcgccgc
     3841 ctcgcgtccg ggcaacgaag caggattcgc caagcgagat ccgatccttc acccaacccg
     3901 atggccgctc ggatcgcaga ctctcgatct cccgaaggtc gtcgttcgag gccgtgcgca
     3961 cgccgatatt gccgtccgcg gcggcgtgag gtccggactc cacgtcgccc cgatagaggc
     4021 agagcacctc gaagccaata gtccttcgga gaaccttgag cgtgaagaag cgcacgaagc
     4081 gcacgccgcc ctgtgtcctg ccgtggaccg aagccagcgt tcgccaagct tctcggaggc
     4141 ggcccaaccg attcgacccg ctgtcaggca tcagtcgtcg ttagttcccc attcgcggaa
     4201 ggcgcgccgc gccggctcgg cgcagcagcc tcttcagcgc tcgtttcgcg gcgtacgcgt
     4261 gatatcgcgc gcgagaggcc gccggcacgc ggaatgcgaa tccttcttcc atcgcatcta
     4321 tgcacgggtg gcaggtgccg cagggcttcc cgcgacgcgg acggtgacag aaccacgacc
     4381 gcagaagaat gtcgcagaac ccgtggcgcc gggacgcgtc gagcatctgg cgcttggtct
     4441 ggtggtacaa cgggaaacgg aagtgcctga agagcagcag gtccgcgtcc ggcacgcgtt
     4501 cgtcgagctc gagggcaccg cctcgctcta ccagccatct gccgagatag gcgaacacgg
     4561 ggtcccttgg gcgatactcg ttcctctcga tggcgagctc gaggccggtc atgccgctcg
     4621 tggcggcgag gcggggaagc cactcgaact ggccccccag acggaaacgc gaggacagag
     4681 accggtacct agccgtaacc tcctccagta ccgggatctc cgacaccaga tagagctgtg
     4741 tcggtcgcag gaggccaacc gccgccgggt gcctggcggc tactgcgtct cgcacagcca
     4801 gcatcgctgc cagctcgtgg agcgaggact ggcggagatg atcgagagcg taaatgggct
     4861 gaaccggcct ggattcgacc accgctaggt gcagcagccg ataggtcgaa tcccaacccc
     4921 cgctccacaa gagcgccacc ggttgatcat ccacatggcg tgtgttcatg gctgttgtgc
     4981 tgacgaggct atccgccctt gtctcgctcc gaagccagcg ccggcggggg gaccaataag
     5041 ctgcaagcgc gccgtcacgg ctacgctcac cagcatatag ccgaccagca cggacagcga
     5101 ggggttcatg cagcctcaaa gaggaagacg cctggctgca ggacttcgcg aacttcggcg
     5161 acgcgacggc gatcattcgt cggtgggttg attggtacaa cgaggcccga ctgcgccaga
     5221 cgctgggcta tcggagtcct cgcggatttc gagcgcagtt gccaaccgcg gaggcttgac
     5281 tcgcggagag cgcgacacag cggctcgggc gtggaagtag cgtcggccat ggtggacatg
     5341 ggtatccgcc ttttggagga tgtccgccgg gtcggatcaa ggccgcctgg agtcccgtgg
     5401 tcctggtgcg agtgctgtcg tttctgtcgc tggccggatg cccagctgat cgggcttagc
     5461 gttccgcgcc ggttgcttga ctgaatgaac gacgggatgg cgtctaccct tcgcggtgtc
     5521 cttgccaggg ccagggcatc ggagttcctc aggaacatcc tcctcgtcat ggccggttcg
     5581 ggcgccgcgc aggccatcag catcgcgctg gtgcccgtca tcagccgcct cttcactccc
     5641 tcggacttcg gcgtttcggg atcgttcgcg gccgtgtcgg gcgtcattgc ggccgccgtg
     5701 acgctcgagt acagccaggc gatcatgctc ccgcgggaga agacggacgc catcggcttg
     5761 ctggcggtgt cgttggccag cacgggcgtc gtcaccctcc tcacactgac ggcgtgtgtc
     5821 ctggcaccct cgctcgtcaa cggcttgatc aagacgcagg gtgcgctggt gttggtcctc
     5881 ctcgtttcgg cgacgctcat cagcggcacg aacaccgcct tgcaggcgtg ggcggtgagg
     5941 gccaaggcat tcaggcacac gtcggcttcg caggttgtca ggagcctctc gggcaagtcg
     6001 gcgacaatcg gctttggact gctcgggact ggggcccccg gcttgatcgc aggggagatc
     6061 ctcggcaacg tcgccgccag catcaacgtc ctcagggtgt tgctgcccga tctcggactg
     6121 ctcgcgcggc aggcaaggcg cgcctcgatg gtcaggctgg cgaaggagta ccgcgacttc
     6181 ccgatgtata cggcgtccca gggcgtcatc aatgcgctgt ccagcggcct gcccgtcctt
     6241 ctgctgacgc gcttcttcgg cttgccagtc gccggctcct acgccttcgc cttgagcgtg
     6301 ttgacgttcc caatggggct cgtgctcagc gcgcttcgcc aggtgctctt ccagaaggcc
     6361 agcgaaagcc agcaccaggg ccgcagcctg tcagcgctgt atgtcagggt cacggccacc
     6421 ctcttcgcaa tggccttgct gcccaccgcg gtcattctcg tctggggccc gcagctgttc
     6481 agcctggtgt tcggcacccg gtggtacacg gccggcgagt tctcgcgaag cttgatgatc
     6541 tggatggcgg tggtcttctg caacctcccg gccacgttgt tcggccggat catcagaatc
     6601 cagcggttcg tcttcttcta cgacctgagc ctgctcgcgc tcagaacggc ggcgctggtc
     6661 ctgggaggct atttcctgtc ggcttcgcag acggtcatgg cctacgctgt cgtcggagcg
     6721 gcgatgaacg cgtatctgat tcttgccgtc gggcgtgccg tcatgaagaa ggaacttgcc
     6781 atgcgcccgg aggggatcct gagctacctg gcgggggact agcccgcgcg gattgtccag
     6841 cgcgccccgg tgatcgcagg acggcgtcgt gcggcagcac gacaagcctg tagtagagga
     6901 gcttcacgca ctgctgcacc acttcttgaa tcccgtgcca gcttgcggtc cagttcttca
     6961 caccgcggga gccttggaca ggagcacaat gcaccgcgat cccgccccgg ccgagcgtgg
     7021 cggcgtagac gagcgctgag cgccggctcc tgaagagcgg cgtcaccacg atcaccgaac
     7081 ggatgcgctg gttctcgagg aactcccgca cgtcgcgcgc cgcgttcagc gtgatgggtt
     7141 ccatctggcg catgggcacg aactcgacat cgcctactcc cgagatgcgg accatcacct
     7201 cggcaacgcc ctgcgcgacg tcgttgagct cgcgcccgtt ccggtcgagc gtgattggga
     7261 cgagcacgcg gggcgccatc ccggcccgcc tgagctggcg cgcgcgctcg aacagcaggt
     7321 agtcgggctc gagattctca atgaggatgg cgtcactggc caccaggctg ggctcgcaga
     7381 cgaggctgcg cgcaatcgct tcggtccacc acccccgccc gagccacagg aggccggcag
     7441 cgaggcccag cgcgccaagc ctgagcttcc acgtggtcct ggtgcgctcg gaccgataga
     7501 agaagtgccg ccgccagccc cggcgcgcac ctggttcgct catgagacgc ccgcccgcag
     7561 atgaggcccg cggcgacgcc ggcgccccgc gcaggacacg catcggcgcc gccggcgcgt
     7621 ccatcatagc ccgggacggc gagcgcgcgg cgccgcccgc cggcgcgggc gccgcgccgg
     7681 cgccgcagcc gacgccgcag ccgacgcccg atccggcgcc gggcgggctg gtcgagctcg
     7741 gccccgcgga cttcgcccgg tgggacgact tcgtcgagcg ccacccggac ggctggatct
     7801 gccacctctc ggactggctg cgcacgctcg aagagtcgtt cccgcacatc acggggcgcg
     7861 tcctcgcctc cgagcgcgac ggcgcgctgc gggcgggcat cccggtctgc gaggtccgga
     7921 gccgcgtgct gggcacccgg ctggtgtcgg tgccctacgg cacgctgtgc tgcccgctgg
     7981 cgccggatga cgaagtgggc cgcctgatgg aaggcgtgct cgacctgggg cgccgcgcgg
     8041 gagcgcgctg catcgaagtc agggccgtgc gccccctgtc cgccgcggcc gccgaacggt
     8101 tcgcgcgaca ggcgcgctac acgcaccaca gcctcgacgt gacggccggc gccgaggcac
     8161 tccgcctccg cctgcaccgc tcctgcgtcc gccagcggat cgaccgcgcg ctggcgcggg
     8221 gcgtggtcga cgaagaggcg caagacgagg gcggcctccg cgccttctac gacctgtacg
     8281 tgcggtgccg gaagcggctg ggcctcccgc cccagcccta tcgcttcatc cgctcgatgt
     8341 ggctggcctt ccggccgcag gggcgcctcg aggtccgcct ggcgtcctgc cgcggcacgc
     8401 cgatcgcagg gatcctcctg ctgcgcttca acggccggac gtcggccgag tacctggtgt
     8461 cggacgacgg gcgccgccac gagagcccgg ggcacctcct cgtctggaac gcgatccggc
     8521 gctcgcacga actgggctgc cgcgttttcg acttcggccg cacggacgcc gccaacgagg
     8581 gcctgatggc gttcaagaac cggtggggcg cgcaagtgac cggcctcccg gtgtactacc
     8641 acccgaagtc gcgcctgcag gcggtggaga gccggcgcgc ggcgctgcgg tcggccggac
     8701 gggtggtgtt caggcgcctg ccggcgcgcg cgaaccgctg gctcggcgaa ctgatctacc
     8761 gccacgcggg ctgacgaacg ggccgcgatc ggacggcggg gcgccgggcc gcgacgaggc
     8821 ggcggcgcgc ccgaccgcgg gccgccggcg cccggaggcc ggaaacgacg aacgcccgag
     8881 cggcacaacc gcggagaacg cctggcatga gcagatggaa cggacggccc ggacgcgagc
     8941 ggcaatggct cggcggcgtg gcgctgctga cgctgctcct ggccctgcct ctcgccaggc
     9001 tggcgatcta cgcctgggac agcagcctgc actcgcacgt cgtgctgatc ccgctggtgt
     9061 cggcctacct gctctacacc cgccgcggca cgctgccggc agccggcccg gcgtcgcccg
     9121 cgggcgcggc cctgccggcg gccatcggcg ccgcggccct cgccgccgcc ttcgcctggc
     9181 gcgaacgcct cagcgtgaac gacggccacg ccctcgtggc gctggcgtac gcgagcctgg
     9241 tcgccgcggg cggcttcctc ttcctcggct cgaagtggat gcgcgcggcg gccttcccgt
     9301 tcgccttcct agtcttcatg gtgcccctgc ccgacgccct cgccgccgcg ctcgaacgcg
     9361 cgtcggccgc ggcctcggcg gaggccgcgg cgttgtactt cgccgccgcc ggcacgccgc
     9421 ttgtccgcca gggcacgatc ttcgagctcc agacgatcac gatggaagtg gcccaggagt
     9481 gcagcggcat ccgctcgagc tgggtgctct tcatcacgag cgtcctggcg gcgcaactct
     9541 tcctcgggag cccgtggagg aggctgatcc tggtggcgtt cgtcatcccg ctgggcatcc
     9601 tgcgcaacgg gttccgcgtg ttcgtcatcg gcatgctgtg cgtgcgcgtg gggccgcaca
     9661 tgatcgacag cctcatccac cggcagggcg ggccgctctt cttcgccctg tcgctggcgc
     9721 cgctgttcct gctgttgtgg tggctgcgcc gcggggagcg gcggcggcga tgacgctgtt
     9781 ccagttcctc ccgttcgccg ccgcggcgct cagcctgctg ctggccgccg ccgcgctcgt
     9841 tcgcaagaag ccgtccgcgg ccacctggtg cttcgtcgcg ggcatggcca tcttcagcct
     9901 cgacagcctc ctgacgggcc tcagcctgcg cgagcccgcg ctggaggcgg ccgccggctg
     9961 gctcacgctg tcgctcctcg ccaagtcgtt cctgccggcc gcctggctgg gcttcggcct
    10021 gacctactcg cgcggcgacc cgcgcgcgtc gctggccgac gcgcggcccg tcgtgacggc
    10081 gctcgcgctg ctgcccgtcg ccatcgccct cgccttccgc ggcagcctcc tccaggcggt
    10141 gccgctcgcg ggcacggccg gcgaggtgct cgtcctgcgc gtgggaacgg ccgccaaggc
    10201 gctcaacgtc gcgctcctcc tgggcttcgc cctggccctg atgaacctgg agcagacgtt
    10261 ccgctcggcc gtcggcacga tgcgctggcg catcaagtgg gtgatcctgg gcctggccgt
    10321 ggtcttcggc ggccaccttt acgtccgcag ccaggcgatc ctcttcccgg tctacgaccc
    10381 ggccctggcc ggcgtcgagt cgagcgccct cctcatcggc tgcgccttcc tcgtcgtcgc
    10441 ctacgcgcgc acgggcctgg ccgaggccga cgtctacccg tcccgcgcgg tcctccgctc
    10501 ctcgctcacg gtcctcatcg tcgggggcta cctggtcgtc gtcggggtgc tcgcgcagct
    10561 cgtgaggcgg ttcggcggcg ccgagagctt ccagctcggc gcgttcgtcg tcctgctggg
    10621 catggcgggg ctgggggtgc tgctgctgtc ggaccggttc cgccagcgca tccaggcgtt
    10681 cgtcgcgcgc cacttcgggc gcgcccagca cgactcggtc agggtgtgga cgcagctctc
    10741 ccagcgcctg gcgaaggtga acgacgaggc cgggctgtgc gaggccgcgg cgaagctggc
    10801 ctgcgaaacg ttcgacgtcc tctccgcgac ggtctggctg atggacgagg cgaagggccg
    10861 gctggcgctc ggggcatcca cctcggcccg cgccgtcgag gccgcgcgcg cgggcccggg
    10921 cgcggcgtcg gccgccgcca tcgccgccgg cctgcagacg cggacggccc ccttcgacct
    10981 agaggcggtc gccgcgccct gggccgacga gtgccgccgc ctcaacccct ccacgttccc
    11041 cacgggcggg cgccgctggt gcgtgccgct gcacacgggc gagcagtgcc tcggcgccct
    11101 cgtgctggcc gaccgcgtca acggcgcgcc ctactcggtg gaggagcggg agctgctggc
    11161 gtgcatcggc gcccaggcga cgtcgttcct gctgaacctg cggctggccc aggaggtggc
    11221 gcgctcgcgc gagctggagg ccttccggac gatgtcggcg ttcttcgtgc acgacttgaa
    11281 gaacacggcg gcctcgctca acctaacgct gaagaacctg ccggtgcact tcgacaaccc
    11341 ggcgttccgc gaggacgcgc tgcgggcggt gggcaacacg gcgcggcgga tcgaggagct
    11401 catcgcgaag ctgagcgcgc tgcggcagcg cccggagctg aagcccgccc ccctcgatct
    11461 caaccagctc gtcggcgagg ccatcgccgg cgccggcgcc atgccgggcg tggacgtgtc
    11521 ggccgacctc ggggccctgc ccccggtcgc cgccgaccgg gagcagatcc ggagcgtcgt
    11581 cacgaacctc ctcctcaacg cccgcgacgc catcggctcg gcgggcggcc gcatcaccgt
    11641 gcgcacggag cgcctcgacg ggcgggccct gctcacgatc gcggacacgg ggtgcggcat
    11701 gagccccgag ttcgtgagag actcgctctt ccgccccttc ctgagcacga agaagcacgg
    11761 cctcggcatc ggcatgttcc aggccaggat gatcgtcgag gcgcacgggg gcacgatcca
    11821 ggtggacagc gaacccggca ggggcacgac gttccgcgtg agcctgccgg ccggacacga
    11881 gccatgacca agccggcgct cctcatcgtc gacgacgatg aagacatcag gacgcagatg
    11941 aggtgggcgc tcgccgacga gtacgaggtg gccgcggcgg gcgaccaggc gggcgccctc
    12001 gcggcgttct cgcagcatcg ccccgccgcc acgctgctgg acctcggcct gcccccgcag
    12061 ccgaacggcc cggacgtggg cctggcgacg ctctcggccc tcatcggcct cgaccacgag
    12121 gccaaggtca tcgtcgtctc ggggcagggc gaccggcaga acgccctcaa ggccgtcggc
    12181 tcgggcgcct acgacttcct gtgcaagccc gtggacatgg acgagctgaa gctcgtcctg
    12241 aagcgctgcc tctacgtggc cggcctcgag cgcgacttcc gggcgctgca ggagcaggcg
    12301 cggcccgagt cgttcgacga catgctcggg gcgagccccc tgatgcacgc cgtgttcgac
    12361 ttcatccgca aggcggccac caccggcgcg cccgtgctca tcctcggcga gaacggcacc
    12421 ggcaaggaga tggtggcccg ggcgctccac cggcgcggcc ggcagcggtc ggggccgttc
    12481 gtgcccatca actgcaacgc catccccgag aacctgctcg agtcggagct gttcggccac
    12541 gaaaagggat ccttcaccgg ggcgcacgcc acgcggaagg gcctggtcga gagcgccgcc
    12601 ggcggcacga ttttcctgga tgaaatcggc gaactgcccg cctcgctgca ggtgaagctg
    12661 ctgcggttcc tgcaggagaa gacgttccag cgggtcggcg ggcggcagga gctgcaaagc
    12721 gacgcccggg tcatcgcggc gacgaacgtc aacctgcagg aggccgtcgg gagcggcaag
    12781 ttcagggagg atttgtactt ccggctggcc gtgctggtcg tcaccatgcc gccgctgcgc
    12841 gagcgcgggg gggacgtcgg acttcttgcc agggagttcc tgcgccgctt cgccgcgcag
    12901 cacggaaggc cggggctgac gttcgccctc gacgccgtcc gggcgctcaa cgcgcacgcc
    12961 tggccgggaa acatccgcga actgcagaac cgcatccagc gggcggccat catggccgag
    13021 ggcaagcggg tgacggccgc cgaccttgag ttggctgcgc ccggcgacgc gcgccggacg
    13081 cagacgctgc gcgaggcccg cgagcaggtc gagcggcagg tcgtcgagga cgccctccgg
    13141 cggcacgggg ggaagatcac ggcggcggca gccgagctcg gcgtaagcag gccaacgctt
    13201 tacgagctga tggagaagct ctccatctcg aagaccgaag gcggccctgc gggcgtctcc
    13261 agggattagc ggatgaacgt gctcgtcacc ggcggcgccg ggttcatagg ctctcacgtc
    13321 gcggaacggc tggtcgcccg gggccgccgg gtcgtggtgc tcgacgacct gagcggcgga
    13381 ttcgccgaga acgtgccggc gggagccgaa ctggtccggg ggagcatcac cgatgccacc
    13441 ctggtggacc gtctgttcga ggcgaaccgc ttcgagcacg tcttccacct ggcggcgtat
    13501 gccgccgaag gcctgagcca cttcatcagg cgcttcaact acacgaacaa cctcaccggc
    13561 agcgtcacgc tcgtcaacgc cgcggtcaac cacggcaccc ggtgcttcgt cttcacctcc
    13621 tccatcgcgg tgtacggggc cagcccggag ctgccgatga ccgaggcgac gccgccgcac
    13681 ccggaagact cgtacgggat cgcgaagctc gccgtggagc aggacctggc ggcggcgcgc
    13741 gcgatgttcg gcctcgactt cgtcgtgttc cggccgcaca acgtctacgg cgaacgccag
    13801 aacatcggcg accgctaccg caacgtggtc ggcatcttca tgaaccaggc gctggcgggt
    13861 cggccgatga cggtgttcgg cgacggcatg cagacgcgcg cgttcagcta catcgacgac
    13921 gtggcgccga tcatcgccga gtcgatcgac gtgcccggcg cgtacaacca ggtgttcaac
    13981 atcggcgccg agcagccgtg ccgggtgaac gacctggcgg cggcggtcgc gcgggcgatg
    14041 ggcgtgccgc cggccgtcgt gcatctgccg gcccggcagg aggtccagga cgcgtactcg
    14101 gcgcacgaga agctggcacg cgtcttcggc acacggccgc cctgcggcct ggaagaaggc
    14161 ctcgagcgga tggcacggtg ggtgcggcgg cagggcgcgc ggcgcggcca gcggttcgag
    14221 gcaatcgagg tgacgcgcaa cctgccggcc gcgtggctgg actgacccgt gggaccgctg
    14281 cccctcgtcg cctgcgtcat cctcaactcg aaccgcaggg cggacactct cgagtgcgtg
    14341 ggatcgctgc tggcaggctc gtactcgaac ctgcgtgtgt tcgtgctcga ttgccagtcg
    14401 cgcgacggat cggtggaggc ggtgcgcgaa gcgttcccgt ccgtcgcggt cgtcgaactc
    14461 gaccgcaacc tgggctatgc cggcaacaac aacgtcggca tccggctggc gctcggcgag
    14521 ggcgccgggt gggtcttcgt cctgaacgag gacacagtcc tcgccgcaga ctgcctcgag
    14581 cggatggttc aggcgatcgt ggtgcgcccg gacgtgggca tcgcgggccc gatggtgtac
    14641 caccacgacg agccggacgt aattcagtcc gctggcggcg gactgacccg gttct
//
LOCUS       DAOSIF010000021        14651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4220_length_14651_cov_3.782954, whole genome shotgun sequence.
ACCESSION   DAOSIF010000021 DAOSIF010000000
VERSION     DAOSIF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14651
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4220_length_14651_cov_3.782954"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLT35_01625"
     CDS             <1..609
                     /locus_tag="PLT35_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG27952.1"
                     /translation="WQCRNWLYFTWTSGDHIVEQHVHNIDVVNWAMGAMPKNVMAMGG
                     RQVRVAPQFGNIFDHFATEFEYPNGVRVLSMCRQTKGCNDRVEERIVGTKGVAWSTAS
                     ATRITGASPWTFEAEEVNPYVQEHIDLIASIRKGAPLNEGRQVAESTMCAIIGRMSAY
                     TGRAISWDWAMNTSTLDLSPKAYEFGPNPVDPVAIPGLTELV"
     gene            736..2844
                     /locus_tag="PLT35_01630"
     CDS             736..2844
                     /locus_tag="PLT35_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG27953.1"
                     /translation="MHLWGLHVVDVAIIAAYLAAVLWIGRKVGASSEGMDDFFLAGRA
                     LGRVYQFFLNFGCSTNADQAVAVSREVYRQGVGGMWIQSLVLFLTPFYWFSTMLFRRV
                     RLTTIGDYYEDRFGSPFLAAAYAVFTLAISAFIGGGIGMLVAGKTMVAMTPKAVAECT
                     PAERASVAEFQEFRELEAARAGGLTPDQHARWEVLRQKHQRGELRSFVSHVNQTWFFL
                     IYAVVVGAYTMMGGFRAAAITDGIQGVLIVVFSVMLVPVGLARLGGFAGLHAAVPAHM
                     FQLFGSASLSDYAWYTVLAMVLANLVSIVAVVSGMQTAGSARNEFTARVGMIGGMFAK
                     RVLMLFWALAGLIAIGLYAGRLHDPDLIWGVMTRDLLWPGAIGLMLIGVLAANMSTLD
                     ASAVSHAALFIRNLYRPIVPGRSDRHYLVVGRAVIAVTLAGAILVAMTAENLLGLFQY
                     IISIPAIFGASIWLGFVWRRVTKWAVILQVALCILLYAAIPTAFERIDAARRYGPWLV
                     ETAPRTVVSTSPATNEDVASGRAARPGEPVTRTRTMPPVGVFFEKVVRQDPEDPRSPK
                     EGLGRFHAELWVMSWFGVDLKTWSKPQLVAARFYFDALFPFLALVLLSLVTRPEPSDA
                     LDRFFARIHTPVQPTPEADAAAVADACANPRKYDGDKWFGGRNWEILRPARSDYLGFF
                     GTWVLVGLIILLLWAMVTIR"
     gene            2841..3872
                     /locus_tag="PLT35_01635"
     CDS             2841..3872
                     /locus_tag="PLT35_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOG27954.1"
                     /translation="MTGDTAPGRRRVGLDNYGLFPLDLSPMATLEWAAARGADGVAFS
                     GLTPEWQARTGEDALKDLRAFAGACDMYLEWGGAQHIPRDMTTWARRDLFGVNEWAAR
                     GAAAVGASVVRSCSGGLMRWNAASPPTELLLRETASALRPQAPMLRDYGVTLAVETHF
                     EFTSFELLRLFDMCGAEPGDWIGVCLDTMNLLTMIEDPVRASARLLPWVVSTHVKDGG
                     VLPSPGGIVTFPAPIGEGVIDLRAIIRLLDDLPRAVHLSVEDHGGSFEVPIRDEEFLS
                     RFPDVDRAELNAVLELARRSEAKADCRPVDRDAWPALCESRLAEDLAALRAMAGPRPA
                     PAPAGAPEA"
     gene            3877..5865
                     /locus_tag="PLT35_01640"
     CDS             3877..5865
                     /locus_tag="PLT35_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27955.1"
                     /translation="MVDVAEHAYRDDPARGHPDVRTRLSGTSYFFLGNGRIQAAVQHA
                     PAGEGTPLGLLVMDPDRLRPKRGALTMHPEHGLEPTALRIAAGTEVRAPEAGAVAVAW
                     QAGAAIPTVVASWKAGPIDVEERFSCPDGERPSLSRDVTLANAGHETIAGTLRTGTPG
                     TEASVAFSLAPGDRLRAALVYDLDRAAGSVNVRIAAPGGGVRPPGPRPERTTVEFGDP
                     VLDRLFRASAAQLPAAVSAAGRMDGSIWQYNREWVRDQAFAALALTMLGHRPLARTML
                     RRLAIEFVTEEGATLDSSQARGRGDVELDQNGILLHVLGEYTAWTGDLGLAEEVWDHV
                     AAAAEYPLRAELRHPASGLLCGTREYWERHAAHGIKPGLELAHQLFVSLGLAAAASLA
                     RQLGRPQPAARWQHESESLRDAMLSDPVFALSDARGFVKRRLVTGQVQETILPSPRAG
                     LPEGVPLAGDPPHRLNPDTSCVLPIAFGTVAPRSAVARATLDHIETLWNQAWHRGGYG
                     RYHVSSEPDSPGAWPFASIFVARAAVEAGDAARAWRILRWLAATDGAASGAWFENNGP
                     RASPPFPQVGITPWTWAELVVLVVQHLLGVRPGPDAVTVTPRLLPGLAGVEARLPVRE
                     GWLRLRLRADPGAPDVATFRVPCGAGETPLEARVRPMP"
     gene            5862..8375
                     /locus_tag="PLT35_01645"
     CDS             5862..8375
                     /locus_tag="PLT35_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOG27956.1"
                     /translation="MTPARRLILVCNSHIDPLWLWPWEEGLAAALATFRSAADLCDEF
                     DGFVFCHNEALLYQWVEEHEPRLFGRIQRLVRLGRWHVSGGWFLQPDCNLPSGESFVR
                     QIGEGLRYFGDRFGVRPRAAFNVDAFGHSRGLVQILAKAGYTGYLFCRPDARHLDLPA
                     DDFVWVGYDGSTLLAHRSAGHYNSELGRARAKVEGWLRTHGADQDGLVLWGVGNHGGG
                     PSRGDLRALAALAGGAHGRHIVHGRPEDYFDGLEARRPSLPRVERDLNPWAPGCYTSM
                     ATVKRAHRRLEALLFATEKTCACAAAQGLLPYPAAGLDEVLQALLFTQFHDVLAGTSV
                     EEAEAQALRRLGLGLDLGARLRARAFFALISNEPQAQEGEYPLFVHNPHPFQVTATVV
                     CEMQPPEPNPDSSVCLGPEVAGRDGRAVPAQVEKESGNIQVDQRKRIVFRPTLEPSAV
                     SRFACRLRKIPRSPAPPLRPVDGVYRHRGAGCAADIDGRTGLLARYCVDGVTYVDRGA
                     FRPLVMRDSADPWGMQARSFRDRAGEFLLLAPRDAAAFAGVAAHELPPVRVIEDGPVR
                     TIVEALFGYGRSVLRLRYALPRQGAEVEVEARATWAETDAMLKLAIPTALGPGRVLGQ
                     VAFGVEEHRRETEELVCQQWVACVSADGAHALTVINDAIYGFDFSGSELRLSCLRSPA
                     YAGHPVDDRTPVVRQDRAEPRVDRGEHVFRFWLAGGPAAERLTVVDREATARHEAPMA
                     LVAFPPGTGGLPALAGAVLSDEAVQMPSLRVIEDGRRLIVRLFEPTGRARTTTLTVPA
                     LGLSLDTRLGPFEIKTLAIDLETRRVCETDLLNAAMEAR"
     gene            8381..9649
                     /locus_tag="PLT35_01650"
     CDS             8381..9649
                     /locus_tag="PLT35_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG27957.1"
                     /translation="MADRKHQRPEPVRVLMAAIGGYGFHYLQALLDETPRHAARLAGV
                     VDPEARASAAWPRVAALGVPVVDAVGAFFDAGHRADLTVVVSPIHLHVPQSAEALERG
                     SHVLCDKPLGASAAAADALAAARDRADRFVLVGYQWSYSAAVQALKRDLLAGRYGRPR
                     RVTTLCAWPRDPAYYARNGWAGRLRDASTGALVLDSPANNAMAHFLHNAFFLVGPEIH
                     RSAMPALVAAEMGRVFSIESADTTACRAETADGCEVLFLASHATERAIEPRFRVECER
                     GSIVFGETRKRIVGTPEGEAPIDYGDPDATPQFQKLFTALGRAVSPGPVVCGIEAAAA
                     QTVCVEAMHASVPEIAPFPAARRRTDPGGRPFVHRLADELERCYAARCLPSELGCAWA
                     RRGRPVVPAVPAGIAAGGSALTPIPGARWP"
     gene            9646..10659
                     /locus_tag="PLT35_01655"
     CDS             9646..10659
                     /locus_tag="PLT35_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOG27958.1"
                     /translation="MTSRELVRRTLRFQKPDRTPRHAWVLPWAEEHSPGPLARLRREF
                     PDDIATVPPLYRAPLPVVGSRYAKGDYVDEWGCRFRNVHGGVIGVTHGPLISDWSQLE
                     GFRAPEAPLAVDADAVRAFCRGTQQFTLAGTLIRPFERLQFIRTVEQAMVDLAEEPPE
                     LLELLRRIHEHYVRETEAWARTDVDAIVIMDDWGGQRRMLASPGTFRRHFRPMYEEYA
                     EIARRYGKFVFMHSDGHILDIVEDLIALGVDALNSQVAVMGVEELGRRFAGRLTFWGE
                     VDRQILLSRGTKDEVARAVREMRAHLYADGGVIAECEFGPGAQPENVLHVFETWRRID
                     EGA"
     gene            10678..13056
                     /locus_tag="PLT35_01660"
     CDS             10678..13056
                     /locus_tag="PLT35_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27959.1"
                     /translation="MTSLLKALSAGLVAASSAGLLFSGGPPAAELPQAAARAQRAAAP
                     AAATERYLAYARAAADWAWDHREENLARWRAQFDPESPFGYRAPGNLLETALIYSYLF
                     EREGRPEHADRAREILVTYGEYRSAFPEWAIRKRPDYDHGVPALSDFFTVMRYLRAFD
                     TLNRLGRLTAADAATIEEVAAHSIEYLLQTSEWGAMNRAMLRAESLAWAVRAFPKHPR
                     AAAWEQQRRALGDDNWGNWEIEDATIYHGVWLYSLLGYADAIGKLDELFRTPEMYYYA
                     HYFLNLMSPAGMVPDFGDAAWTQSWQHFFVFFEAAAARLNDPAMAWAAQEIASRFVDF
                     SAPRNVALGCFLIDASRWGRPGIRPAAPTHLSGEVMEDVQGKKIVFRDGWTPSSTYLL
                     LNYRDEGDGGMNFRDYLRDTIPVEEEKMTHGHADENSIVLLMSNGSVLLRDGGYRDYM
                     PSGPFGAYRQDYFHNRLVVRPEKFFMGQEKGDTRYSSKDPVPGQPILEFLRNAGSYRP
                     VRTRKVDFLSLPEVDYSRTRLIDDGWGYDYDRVIAYVKDPGLFVVFDIFKSRTDEFFT
                     LANLWHTRKIYAQGEHWYDTGYDAVGQIALADTDRLLVVFPATHFRIEGVTPQKRHYQ
                     DELTIHQATGRHFEPTHTEGFITVLVPHAREADPQALAARIRLVEAAPAGAGLGVAIE
                     TGTRRITVGAKQDLRLDIARDYRRPRYLFEKGRVAYGPLVTDGDFVFASEERGEIAYA
                     IVNMTRAQFGEQVLFQNGQSFHGLPFDAAADPAGIDKVRYWRDTVKVQTGGR"
     gene            13046..14647
                     /locus_tag="PLT35_01665"
     CDS             13046..14647
                     /locus_tag="PLT35_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27960.1"
                     /translation="MGVETRIRTGRPEFDGLFPLVRSILERAALELVIDAQHIRGCRT
                     GDARSIWIRDFSDMLRALRHVEPDVTSAVQHFADTQAASGRIFDSVTTFPERPPSERE
                     NWTKYVRVPVEADVEYRFVKAAYLAWQATGDDEWARRLLPSLERAMRYVTGHPWYWDR
                     QRGLVKRPYTIDTWDFAYTAGRHGWLQFQVTDDTYWGCMHGDSSGYVEAFGLMARLHR
                     RLGDARIAARWDAEAATLRAAMNRACWNGRFYTHFVKITPVEVPGVDEGEQLSLSNPM
                     AINRGAADHSQAAAILREYQRRRAEGRAFAEWFSIDPPFPDGVFGDPRLVKGAYVNGG
                     IMPLVGGELARAAFEHGFEQYGIDILRRYYALASERGESFLWYFPDGRPSTVEASTSP
                     EATPADGWGSSAMAWALVEGLAGVVDGGRLFDAVRLSPRWEAAGVNDAEVGVGYAASG
                     AGLEYQYRRRPGLITIDVRKPDGDSADVDWRVLLPHGCRNVAARCDGRSLPVATLQVE
                     ASLYAAFASRLNGETHVELKLGGEA"
BASE COUNT         2004 a         5239 c         5266 g         2142 t
ORIGIN      
        1 tggcagtgcc gcaactggct ctacttcacc tggacctccg gcgaccacat cgtcgagcag
       61 cacgtgcaca acatcgacgt cgtcaactgg gccatgggcg cgatgccgaa gaacgtgatg
      121 gcgatgggcg ggcggcaggt gcgcgtggcg ccccagttcg gcaacatctt cgaccacttc
      181 gccacggagt tcgagtaccc gaacggggtg cgggtgctga gcatgtgccg gcagacgaag
      241 ggctgcaacg accgggtcga agagcggatc gtgggcacga agggcgtggc gtggagcacc
      301 gcgagcgcca cgcgcatcac cggcgcgtcg ccgtggacct tcgaggccga ggaggtcaat
      361 ccctacgtcc aggagcacat cgacctcatc gcgtcgatcc ggaagggcgc gccgctcaac
      421 gaggggcgcc aggtcgccga gagcacgatg tgcgccatca tcggcaggat gagcgcctac
      481 acgggccggg cgatctcctg ggactgggcg atgaacacct cgacgctcga cctgtcgccc
      541 aaggcgtacg agttcgggcc gaatcccgtg gacccggtgg ccatccccgg cttgacggaa
      601 ctcgtctagc cggcgcgccg tgacgggcgg ggccggctcg gcggccggcc tccgccgcgg
      661 cgttcggtcg tcggccgtgc gccggaagcc cgcttgcccg agctcccggc ttcgacccat
      721 cgaggcgcgt ccgccatgca cctgtggggc ctccacgtcg tcgatgtggc catcatcgcc
      781 gcctacctgg ccgcggtgct ctggatcgga cggaaggtcg gggcctcgtc cgagggcatg
      841 gacgacttct tcctggccgg ccgcgcgctg ggaagggtct accagttctt cctgaacttc
      901 gggtgctcca ccaacgccga ccaggcggtg gcggtctcgc gggaggtcta ccgccagggc
      961 gtcggcggga tgtggatcca gtcgctggtg ctgttcctca cgccgttcta ctggttcagc
     1021 acgatgctgt tccgcagggt gcgcctgacc accatcggcg actattacga ggaccgcttc
     1081 ggcagcccgt tcctggccgc cgcctacgcg gtcttcaccc tcgcgatctc ggccttcatc
     1141 ggcggcggca tcggcatgct cgtcgccggc aagacgatgg tggccatgac gccgaaggcc
     1201 gtcgcggagt gcacccccgc cgagcgggcg agcgtcgcgg agttccagga gttccgcgag
     1261 ctcgaggcgg cgcgggcggg cgggctcacg ccggatcagc acgcgcgctg ggaggtcctc
     1321 cggcagaagc atcagcgcgg cgaactccgc tccttcgtgt cccacgtcaa ccagacgtgg
     1381 ttcttcctca tctacgccgt cgtcgtcggc gcgtacacga tgatgggcgg cttcagggcc
     1441 gccgcgatca cggacggcat ccagggggtg ctgatcgtcg tcttctccgt catgctcgtg
     1501 ccggtcggcc tcgccaggct gggaggcttc gcggggctgc acgccgccgt gccggcccac
     1561 atgttccagc tgttcggatc ggcgtcgctc agcgactacg cctggtacac cgtgctcgcg
     1621 atggtgctgg cgaacctggt gtccatcgtc gccgtcgtgt cgggcatgca gacggccgga
     1681 tcggcgagga acgagttcac cgcgcgcgtg ggcatgatcg gcggcatgtt cgccaagcgc
     1741 gtcctgatgc tcttctgggc gctggccgga ctcatcgcca tcggcctcta cgcgggccgg
     1801 ctgcacgacc cggatctcat ctggggcgtg atgacccggg acctgctgtg gccgggcgcg
     1861 atcggcctca tgctcatcgg cgtgctggcc gcgaacatgt ccacgctgga cgcatcggcc
     1921 gtctcgcacg cggcgctgtt catcaggaac ctctaccggc ccatcgtgcc gggcaggagc
     1981 gaccggcact acctcgtcgt cgggcgggcc gtgatcgccg tgacgctggc cggggcgatc
     2041 ctggtggcca tgacggcgga gaacctgctc ggcctgttcc agtacatcat ctcgatcccg
     2101 gcgatcttcg gcgcgtccat ctggctgggg ttcgtctggc gccgcgtgac gaagtgggcg
     2161 gtcatcctgc aggtggcgct ctgcatcctg ctctacgcgg caatcccgac ggccttcgag
     2221 cgcatcgacg cggcgcggcg ctacgggccg tggctcgtcg agaccgcgcc gcgcaccgtg
     2281 gtgtccacgt ccccggcgac gaacgaagac gtcgcctcgg gccgcgcggc caggccgggc
     2341 gaacccgtta cgcggacgag gacgatgccg ccggtcggcg tgttcttcga gaaggtcgtc
     2401 cgtcaggacc ccgaggaccc gcggtcgccg aaagaggggc tcggccggtt ccatgcggag
     2461 ttgtgggtca tgagctggtt cggcgtcgat ttgaagacgt ggtcgaagcc gcagctcgtg
     2521 gccgcgcgct tctacttcga cgccttgttc ccgttcctcg cgctcgtcct gctctcgctc
     2581 gtcacgcggc ccgagcccag cgacgcgctg gaccgcttct tcgcccggat ccacacgccg
     2641 gtgcagccga cgccggaggc agacgcggcc gccgtcgccg atgcgtgcgc gaacccgcgc
     2701 aagtacgacg gtgacaagtg gttcggcggc cggaactggg agatcctccg gccggctcgc
     2761 agcgactatc tcgggttctt cggcacctgg gtgctcgtcg gcctgatcat cctgctcctg
     2821 tgggcgatgg tgacgatccg atgacgggcg acacggcgcc cggccgccgc cgggtgggcc
     2881 tcgacaacta cgggctgttt ccgctcgatc tgtccccgat ggcgacgctc gaatgggccg
     2941 cggcgcgtgg cgccgacggc gtcgccttct cgggcctgac tccggagtgg caggcgcgca
     3001 cgggcgaaga cgcgctgaag gacctccgcg cgttcgccgg cgcctgcgac atgtacctcg
     3061 agtggggcgg cgcccagcac atcccccgcg acatgacgac gtgggcgcgc agggacctgt
     3121 tcggcgtcaa cgaatgggcg gcgcgcggcg cggcggccgt tggggcgtcg gtcgtgcggt
     3181 cctgctcggg cggcctgatg cgctggaacg cggccagccc gcccaccgag ctgctgctgc
     3241 gcgagacggc gtccgccctc cgcccgcagg cgcccatgct gcgcgactac ggcgtgacgc
     3301 tggccgtcga gacgcacttc gagttcacct ccttcgaact cctccggctg ttcgacatgt
     3361 gcggcgcgga accgggcgac tggatcggcg tctgcctcga cacgatgaac ctcctgacga
     3421 tgatcgagga cccggtccga gcgtcggctc gcctgctccc gtgggtggtc agcacgcacg
     3481 tcaaggacgg cggcgtcctg ccgtcgcccg gcggcatcgt cacgtttccg gcgccaatcg
     3541 gcgagggcgt catcgacctc cgggccatca tccggctcct ggacgacctg ccgcgcgcgg
     3601 tccacttgtc cgtggaagat cacggaggga gcttcgaggt gccgatcagg gacgaggagt
     3661 tcctctcgcg gttccccgac gtcgatcgcg ccgaactgaa cgccgtgctc gagctggccc
     3721 ggcggtccga agccaaggcg gactgccgcc cggtcgatcg agacgcgtgg ccggcgttgt
     3781 gcgagtcgag gctcgccgag gacctcgccg cgcttcgggc gatggccggg ccgaggccgg
     3841 cgcccgcccc cgcaggcgcg ccggaggcgt agcgcgatgg tggatgtcgc cgagcacgcg
     3901 tatcgagacg accccgcgcg gggccacccg gacgtgcgca cgcgcctgtc agggacgtcc
     3961 tatttcttcc tgggcaacgg ccgcatccag gccgccgtgc agcacgcgcc cgccggcgag
     4021 ggcacgccgc tcggcctgct cgtcatggac ccggatcggc tgcggccgaa gcgcggcgcg
     4081 ttgaccatgc acccggagca cggcctcgag ccgacggccc ttcgcatcgc cgcgggaacc
     4141 gaggtgcgtg cgccggaggc tggggcggtc gcggtggcgt ggcaggctgg agcggcgatc
     4201 ccgacggtcg tggcctcgtg gaaggcgggg ccgatcgacg tcgaagaacg cttctcgtgt
     4261 cccgacggcg agcggccgag cctgtcccgg gacgtcacgc tggcgaacgc cgggcacgag
     4321 accatcgcag ggacgctgcg cacgggaacg ccaggcacgg aggctagcgt cgccttctcg
     4381 ctggcccccg gcgatcgcct ccgggcggcc ctcgtctacg atctcgaccg ggcagccggc
     4441 tcggtgaacg tgcgcatcgc cgcccccggc ggcggggttc gcccgcccgg cccgcggccg
     4501 gagaggacca ccgtcgagtt cggcgacccc gtcctcgacc gcctgtttcg cgccagcgcc
     4561 gcgcagctgc cagccgccgt gtcggctgcg ggccgcatgg acggcagcat ttggcagtac
     4621 aaccgcgaat gggtccgcga ccaggcgttc gcggcgctcg ccctcacgat gctcgggcac
     4681 cggccgctgg cgcggacgat gctgcgccgc ctggccatcg agttcgtgac cgaggagggc
     4741 gcgacgctcg attcgagcca ggcccgaggg cgcggcgacg tggaactcga tcagaacggc
     4801 atcctgctcc acgtgctggg cgagtacacc gcctggacgg gcgacctcgg gctcgccgag
     4861 gaggtctggg accatgtcgc ggccgccgcg gagtacccgc tgcgcgcgga gctccgccac
     4921 ccggcgagcg gcttgctttg cgggacgcgg gagtactggg agcggcacgc cgcgcacggc
     4981 atcaagcccg gcctggaact ggcccaccag ctgttcgtgt cgctcggcct cgccgccgcc
     5041 gcctcgctgg cgcggcagct cggccgccca cagccggctg cacgctggca gcacgagtcg
     5101 gagtcgctgc gcgacgcgat gctctccgac ccggtgttcg ccctgtccga cgcccgcggc
     5161 ttcgtgaagc ggcggctggt gacgggccag gtgcaggaga cgattctgcc tagcccccgc
     5221 gcgggactgc ccgagggcgt tccgctcgcc ggcgacccgc cgcaccggct gaaccccgac
     5281 acgagctgcg tcctgccgat tgccttcggc accgtcgcgc cgcgctcggc ggtggcgcgc
     5341 gcgacgctgg atcacatcga aacgctgtgg aaccaggcgt ggcaccgggg cggctacggc
     5401 cgataccacg tctcgtccga acccgattcc cccggcgcgt ggccgttcgc gtcgatcttc
     5461 gtcgcgcggg cggccgtcga ggccggcgac gccgcgcgcg cctggcgcat cctgcgctgg
     5521 ctcgccgcga cggacggcgc cgcctcgggc gcgtggttcg agaacaacgg acctcgcgcg
     5581 tcgccgccgt ttccgcaggt cggcatcacg ccgtggacgt gggccgaact cgtcgtcctc
     5641 gtcgtgcagc acctgctcgg cgtgaggccg ggcccggacg cggtcacggt gacgccgagg
     5701 ctcctgccgg ggctggccgg cgtcgaggcg cgcctgcccg tccgcgaggg ctggctgcgg
     5761 ctccggctcc gcgccgaccc aggcgcgccg gacgtcgcga cgttccgcgt gccctgcggc
     5821 gccggcgaga ccccgctcga ggcgcgagtg aggccgatgc catgacgcca gcgcgccgcc
     5881 tgatcctcgt ctgcaattcg cacatcgacc cgctgtggct gtggccgtgg gaagaagggc
     5941 tggcagcggc gctggccacc ttccggtcgg cggccgatct gtgcgacgag ttcgacggct
     6001 tcgtcttctg ccacaacgaa gcgctcctgt accagtgggt agaggagcac gagccgcgcc
     6061 tgttcggccg catccagcgc ctggtgcgcc tgggacggtg gcacgtgtcg ggcggctggt
     6121 tcctccagcc ggactgcaac ctgccgtcgg gcgaatcgtt cgtgcgccaa atcggcgaag
     6181 gccttcggta ctttggcgac cggtttggcg tgcggccgcg cgcggcgttc aacgtcgacg
     6241 cgttcggcca cagccgcggc ctggtccaga tcctggcgaa ggcgggctac accgggtatc
     6301 tcttctgccg cccggacgcg cggcacctgg acctgcccgc ggacgacttc gtctgggtgg
     6361 gctacgacgg gtccaccctg ctcgcccacc gctccgccgg gcactacaac tcggaactgg
     6421 ggcgggcccg cgcgaaagtc gaggggtggc tccgcacgca cggcgccgac caggacgggc
     6481 tcgtgctgtg gggcgtgggg aatcacggcg gcgggccgtc gcgcggcgat ctccgcgccc
     6541 tggcggcgct cgcgggcggc gcgcacggcc ggcacatcgt gcacgggcgg cccgaggact
     6601 acttcgacgg cctcgaggca agacgtccct cgctcccgcg cgtcgagcgg gacctcaacc
     6661 cctgggcgcc cgggtgctac acgtcgatgg cgacggtcaa gcgcgcgcac cggcgcctcg
     6721 aggcgctcct gttcgccacg gagaaaacgt gcgcgtgtgc cgccgcccag ggcctgctgc
     6781 cctaccccgc cgccggcctg gacgaggtgc tccaggctct gctgttcacg cagttccacg
     6841 acgtgctcgc gggaacgagc gtcgaggagg ccgaggcaca agcgctgcgg cggctgggac
     6901 tcggcctgga cctcggtgcc cggctgcggg cgcgggcgtt cttcgcgctg atctcgaacg
     6961 agccgcaggc gcaggagggc gagtacccgc tgtttgtcca caacccccac ccgttccagg
     7021 tgaccgccac ggtcgtctgc gaaatgcagc cgcccgagcc caaccccgat tcgtccgtgt
     7081 gcctcgggcc ggaggtcgcc ggccgcgacg ggcgcgccgt gccggcgcag gtcgagaaag
     7141 agagcggcaa catccaggtg gaccagcgca agcggatcgt cttccgcccg acgctcgagc
     7201 cctcggcggt gagccgattc gcctgccggc tgcgcaagat cccgaggtcg cctgcgccgc
     7261 ccctgcgtcc ggttgacggc gtgtaccggc atcgcggcgc cggctgcgcc gcggacatcg
     7321 acggccgaac gggactgctc gcgcgctact gcgtggacgg cgtcacgtac gtcgatcgcg
     7381 gcgccttccg tcccctcgtc atgcgcgact cggctgaccc gtggggcatg caggcgagga
     7441 gcttccgcga ccgcgccggc gagttcctgc tcctggcgcc gcgcgacgcc gcggcgttcg
     7501 ccggcgtcgc ggcgcacgaa ctgccgccgg tccgcgtcat cgaagacggc ccggtccgca
     7561 caatcgtcga agcgctgttc gggtacggcc gttccgtcct gcgcctgcgc tacgcgctgc
     7621 ccaggcaggg cgcggaagtc gaggtcgaag cgcgcgccac ctgggcagaa acggacgcaa
     7681 tgctgaagct ggcgatcccg acggccctcg gacccgggcg ggtgctcggc caggtggctt
     7741 tcggggtcga ggaacaccgt cgcgagaccg aggaactcgt ctgtcagcag tgggtggcgt
     7801 gcgtctcggc ggacggcgcg catgccctga cggtcatcaa cgacgcgatc tacgggttcg
     7861 acttctccgg gtccgagttg cgcctgtcgt gtctccgctc gcccgcgtac gccggccacc
     7921 cggtggatga ccggacgccc gtcgtcaggc aggaccgggc cgagcccagg gtcgatcgcg
     7981 gcgagcacgt gttccgcttc tggctcgccg gcgggccggc cgccgagcgc ctgaccgtgg
     8041 tggatcggga ggccacggcg cggcacgagg ccccgatggc cctcgtggct ttcccgcccg
     8101 gaaccggcgg gctgccggcc cttgccggcg ccgtcctgtc cgacgaagcg gtccagatgc
     8161 cgagcctgcg cgtcatcgag gacgggcgcc ggctgatcgt ccggctgttc gagccgacgg
     8221 ggcgcgcgcg gaccaccacg ttgacggtgc ccgcgctggg cctgtccctc gacacgcgcc
     8281 tgggaccgtt cgagatcaag acgctggcga tcgatctcga gacaaggcgg gtatgcgaga
     8341 ccgatctgct gaacgcggcc atggaggcgc ggtagcggac gtggcagaca ggaagcacca
     8401 acgcccggaa cccgtcaggg tgctgatggc cgccatcggc ggatacggat tccactacct
     8461 ccaggcactg ctggacgaga cgccacgcca cgcggcgcgc cttgccggcg tcgtggaccc
     8521 cgaggcgcgc gcgtcggcgg cgtggccgcg ggtcgccgcc ctcggcgtgc cggtcgtgga
     8581 cgcggtcggc gcgttcttcg atgcgggcca cagggccgat ctcacggtcg tggtctcgcc
     8641 gatccacctc cacgtcccgc agagcgcgga ggccctcgaa cgcgggagcc acgtgctctg
     8701 cgacaagccg ctcggcgcgt cggcggccgc cgctgacgcc ctggccgctg cacgagaccg
     8761 ggccgaccgg ttcgtcctcg tcggctacca gtggtcgtac tcggcggccg ttcaggcgct
     8821 caagcgcgac ctgctcgccg gccgctacgg gcggcccagg cgggtcacga cgctgtgcgc
     8881 ctggccgcgg gaccctgcct actacgcgcg caacggctgg gccggccggc tgcgcgatgc
     8941 ctcgaccggc gcgctggtcc tcgacagtcc ggcaaacaac gcgatggccc acttcctcca
     9001 caacgccttc ttcctcgtcg ggcccgagat acaccgcagc gcgatgccgg cgctcgtcgc
     9061 cgccgagatg gggcgcgtct tctccatcga gagcgccgac acgaccgcct gccgcgccga
     9121 aaccgcagac ggctgcgagg tgctcttcct cgcctcgcac gccaccgagc gggccatcga
     9181 gccgcgcttt cgcgtggagt gcgagcgcgg gagcatcgtc ttcggagaaa cccggaagcg
     9241 cattgtcggc acgccggagg gcgaggcgcc gatcgactac ggcgaccccg acgccacgcc
     9301 ccagttccag aagctcttca cggccctcgg ccgcgccgtc agccctggtc ccgtggtctg
     9361 cgggatcgag gcggcggccg cgcagacggt ctgcgtggaa gcgatgcacg cgtcggtccc
     9421 cgagatcgcg cccttccccg ccgcgcgccg ccgcacagac ccgggcggcc gcccgttcgt
     9481 ccaccgcctg gcagacgagc tggagcgctg ctacgccgcg cgatgcctcc cgagcgaact
     9541 cggctgcgcc tgggcgcggc gcggccgtcc ggtcgtgccg gcggtgcccg cgggcatcgc
     9601 cgccggcggc tccgcgctga cgccgatccc cggagcgcga tggccatgac gtcgcgggag
     9661 ctcgtccggc ggaccctgcg gtttcagaag cccgaccgca cgccgcgcca cgcctgggtg
     9721 ctcccgtggg ccgaggagca ctcgcctgga ccgctcgccc ggctccgccg cgagttcccc
     9781 gacgacatcg cgaccgttcc cccgctgtac cgcgccccgc tgccggtcgt cgggagccgc
     9841 tacgcgaagg gcgactacgt cgacgagtgg ggctgccgct tccgcaacgt ccacggcggg
     9901 gtcatcggcg tcacgcacgg gccgctcatc tccgactggt cgcagctcga ggggttccgc
     9961 gcgcctgagg cgccgctggc ggtggacgcg gacgcggtcc gggcgttctg ccgcggcacc
    10021 cagcagttca ccctggccgg caccctcatc cgccccttcg agcgcctcca gttcatccgg
    10081 accgtggagc aggcgatggt ggacctggcg gaagagccgc cggagctgct ggagctgctg
    10141 cggcgcatcc acgagcacta cgtgcgcgaa accgaagcat gggcgcggac cgacgtggac
    10201 gccatcgtga tcatggacga ctggggcggt cagcgccgga tgctggcgtc gcccgggacg
    10261 ttccgccggc acttccgccc gatgtacgag gagtacgcgg agatcgcccg gcgctacggc
    10321 aagttcgtct tcatgcactc ggacgggcac atcctcgaca tcgtggagga cctcatcgcg
    10381 ctcggcgtgg acgcgctcaa cagtcaggtg gcggtcatgg gcgtcgagga gctgggccgg
    10441 cgcttcgccg gccgactcac gttctggggc gaggtggatc gccagattct gctgtcgcgc
    10501 gggacgaaag acgaggtggc ccgggccgta cgcgagatgc gggcgcacct gtacgccgac
    10561 ggcggtgtga tcgcggagtg cgagttcggg ccgggcgcgc agcccgagaa cgtgctgcac
    10621 gtgttcgaga cctggcgccg gatcgatgaa ggcgcctgac atcgggaggg tatcgccatg
    10681 acgtccctgc tgaaggccct gtcggcgggc ctggtcgcgg cttcgagcgc aggcctcctg
    10741 ttctccggcg gccctcctgc ggcggagctc ccgcaggcgg ccgcgcgcgc gcagcgtgcg
    10801 gcggcgccgg cggccgccac cgagcggtat ctggcctacg ctcgcgccgc cgccgactgg
    10861 gcctgggacc accgcgagga gaacctcgcg cggtggcggg cgcagttcga cccggagagc
    10921 cccttcggct accgcgcgcc gggcaacctg ctcgagacgg cgctgatcta ctcgtacctg
    10981 ttcgagagag aaggccggcc ggagcacgcc gatcgcgccc gggagatcct cgtgacctac
    11041 ggggagtacc ggtctgcctt cccggagtgg gccatcagga agcggccgga ctacgaccac
    11101 ggcgtcccgg cgctctccga cttcttcacc gtgatgcgct acctgcgggc cttcgacacg
    11161 ctgaaccggc tgggcaggct gacggccgcc gacgcggcga cgatcgagga agtggcggcg
    11221 cactcgatcg agtacctgct ccagacctcc gagtggggcg ccatgaaccg ggccatgctg
    11281 cgcgcggagt cgctggcgtg ggcggtgcgc gcgttcccga aacacccccg cgcggccgcc
    11341 tgggagcagc agcgccgcgc cctcggcgac gacaactggg gcaactggga aatcgaggac
    11401 gcgaccatct accacggcgt ctggctttac tcgctgctgg gctacgcgga cgcgatcggc
    11461 aagctcgacg agctgttccg gacgcctgag atgtactact acgcgcacta tttcctgaac
    11521 ctgatgtcgc cggccggcat ggtgccggac ttcggcgacg ccgcgtggac gcagagctgg
    11581 cagcacttct tcgtcttctt cgaagccgcg gccgcccggc tgaacgatcc ggcgatggcg
    11641 tgggccgcgc aggagatcgc gtcgaggttc gtcgacttct ccgcgccgag gaacgtggcg
    11701 ctcggctgct tcctcatcga cgccagccgg tggggccggc cgggcatccg gcccgcggcg
    11761 ccgacgcacc tgagcggcga ggtgatggag gacgtgcagg gcaagaagat cgtgtttcgc
    11821 gacggctgga cgccctcgtc cacctacctg ctgctgaact accgcgacga gggcgacggc
    11881 ggcatgaact tccgcgacta cctgcgcgat accatccccg tcgaggagga gaagatgacg
    11941 cacggccacg ccgacgagaa cagcatcgtg ctgctcatgt ccaacggctc cgtgctgctg
    12001 cgcgacggcg gctaccgcga ctacatgccg agcgggccgt tcggcgccta ccgccaggac
    12061 tacttccaca accgcctggt cgtccggccc gagaagttct tcatgggcca ggagaagggc
    12121 gacacgcgct acagctcgaa ggacccggtc cccggccagc cgatcctcga gttcctccgg
    12181 aacgccggct cctaccggcc ggtccgcacg cgcaaggtgg acttcctctc gctgcccgaa
    12241 gtggactaca gccggacccg cctcatcgac gacgggtggg gctacgacta cgaccgcgtg
    12301 atcgcctacg tgaaggaccc cggcctgttc gtggtgttcg acatcttcaa gtcgcgcacc
    12361 gacgagttct tcacgctcgc gaacctgtgg cacacgcgga agatctacgc gcagggcgag
    12421 cactggtacg acaccggcta cgacgccgtg ggccagatcg cgctcgccga cacggaccgg
    12481 ctcctcgtgg tgtttccggc gacgcacttc cggatcgagg gcgtgacgcc gcagaagcgc
    12541 cactaccagg acgagctgac gatccatcag gcgacgggcc gccacttcga gccgacgcac
    12601 acggagggct tcatcaccgt cctggtgccg cacgccaggg aggccgatcc gcaggccctg
    12661 gccgcccgga tccggctcgt cgaagcggcg ccggcgggcg cgggcctcgg cgtggccatc
    12721 gagacgggca cccgccgcat caccgtgggc gccaagcagg acctccggct ggacatcgcc
    12781 cgcgactacc ggcggccgcg gtacctcttc gagaagggcc gggtggcgta cggcccgctc
    12841 gtgaccgacg gcgacttcgt cttcgccagc gaggagaggg gcgagatcgc ctatgccatc
    12901 gtcaacatga cccgggcgca gttcggcgaa caggtgctgt tccagaacgg ccagtcgttc
    12961 cacggcctgc cgttcgacgc ggcggcggac ccggccggca tcgacaaggt ccggtactgg
    13021 cgcgacacgg tgaaggtgca gaccggtggg cgttgagacg cgcatccgaa ccggccgccc
    13081 ggagttcgac gggctgttcc cgctcgtgcg ctcgatcctc gagcgggcgg ccctcgagct
    13141 ggtgatcgac gcccagcaca tccggggctg ccggaccggc gacgcgcggt cgatctggat
    13201 ccgcgacttc agcgacatgc tccgggcgct ccgccatgtc gagccggacg tgacgagcgc
    13261 cgtccagcac ttcgcggaca cgcaggccgc cagcggccgc atcttcgact ccgtgacgac
    13321 gttccccgag aggccgccgt ccgagcgcga gaactggacg aaatacgtgc gcgtgccggt
    13381 cgaggccgac gtcgagtacc gcttcgtgaa ggccgcctac ctggcttggc aggcgaccgg
    13441 cgacgacgag tgggcccggc gcctgctgcc gtcgctcgag cgggcgatgc ggtatgtgac
    13501 gggccatccg tggtactggg accgccagcg tggcctcgtg aagcggccgt acaccatcga
    13561 cacctgggac ttcgcctaca cggctggccg gcacggctgg ctgcagttcc aggtgaccga
    13621 cgacacgtac tggggctgca tgcacggcga ctcgtccggc tacgtcgagg cgttcggcct
    13681 catggcgcgc ctgcaccgcc ggctgggcga cgcgcgcatc gcggcgcgct gggacgccga
    13741 agccgccacg ctgcgcgcgg cgatgaaccg cgcctgctgg aacggccggt tctacaccca
    13801 cttcgtgaag atcacgcccg tcgaggtccc gggcgtcgac gaaggcgagc agctcagcct
    13861 gagcaacccg atggccatca accgcggcgc ggccgaccac agccaggcgg cggcgatcct
    13921 ccgggagtac cagcgccggc gcgccgaggg ccgggctttc gccgagtggt tctcgatcga
    13981 cccgccgttt cccgacggcg tgttcggcga cccgcgcctc gtgaagggcg cgtacgtgaa
    14041 cggcggcatc atgcccctgg tgggcggcga actggcccgg gccgccttcg aacacgggtt
    14101 cgagcagtac gggatcgaca tcctccgccg ctactacgcc ctcgcgtccg agcggggcga
    14161 atcgttcctc tggtacttcc ccgacggccg cccgagcacg gttgaggcca gcacgagccc
    14221 tgaggcgacg ccggccgacg gctggggctc gagcgcgatg gcgtgggcgc ttgtcgaagg
    14281 gctggccggc gtcgtggacg gcggacgcct gttcgatgcc gtccgcctgt cgcccaggtg
    14341 ggaagcggcc ggcgtgaacg acgcggaagt gggggtcggc tacgcggcgt cgggagcggg
    14401 actcgagtac cagtaccggc gccgcccggg gctcatcacg atcgacgtcc ggaagccgga
    14461 tggcgactcg gcggacgtcg actggcgcgt gctgctcccg cacggctgcc gcaacgttgc
    14521 cgctcggtgc gacggccggt ccctgccggt cgcgacgctc caggtggagg ccagcctcta
    14581 cgccgccttc gcgagccgcc tgaacggcga aacgcacgtg gaactgaagc tcgggggcga
    14641 ggcatgacgg t
//
LOCUS       DAOSIF010000022        13829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4520_length_13829_cov_4.267243, whole genome shotgun sequence.
ACCESSION   DAOSIF010000022 DAOSIF010000000
VERSION     DAOSIF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4520_length_13829_cov_4.267243"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..4478)
                     /locus_tag="PLT35_01670"
     CDS             complement(198..4478)
                     /locus_tag="PLT35_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOG27961.1"
                     /translation="MTQADALGALCGYAALGIDIGSVNVKVCGLDGRSGAAHAVVPHD
                     GDLETALEQALSAIGAGGPVGPATVATGGAGRRRIDAPDVIAPLAIEAALGALSLRPR
                     AVVSVGGEDLVVYILNARAQVQTTYAGNKCASGTGEFFRQQLGRMDLRLEDLDQAAAS
                     ARVLKLSARCSVFMKSDCTHRLNKGEATKGDIALSLSKVMADKVGEFLIRAKVQAGEV
                     VLVGGVTENAHFVRFLREAWPRTRFIMPAEARYFEAFGAAHLAASLGRPLPPARRRLR
                     ALAAAEGTPFEPLASAASRVTYYPSKRGALRPGAEYILGVDGGSTTTKVALIDAETLE
                     IAAAHYGRTHGDPVAALRRCLREVDGQLGGARPAITLAAATGSSRELLGVFLETEGVY
                     NEIIAHAAGTTYFEPDVDTLFEIGGQDAKYVLLNNGVPIDYAMNEACSAGTGSFLEES
                     AAGDLCVERAEDIGPIALEARAPLKFGEHCSAFINSDIRKAIQNGSDRADIVAGLVFS
                     IVANYLNRVVGNRSVGERIALQGGVAKNPAVPLAFAQLVGKPVSVPPDPELMGCFGVA
                     RLALEKHRAGLLARGSFDLRTLAAKEIETLGEFTCQACDNRCPIRNLSVDGHRYPFGG
                     RCSKFTGARRRLPGSERDAVDHVAWRHDEVFERAAPDPARFRPRTDRVVGVPRALSVH
                     SLWPLYARFFHELGVRAVLSERIAPEGVARQESSYCFPVEIAHGAIQDLLDRRADFLF
                     LPHFRDMPSMESGKVHACTCPLTQGLPYLARQAFGVDATVLRPVVSFKAGWEACRPEF
                     ERVAAQLGFDAAEGGQAFDEAVGSYRAFLERYRQRGREVMDEIRSLPDRLYVGLFGRP
                     YNAFTRDANMGIPRKFTSRGVTVVPFDMIYDESAGIFPNMYWYYGQQDMKAAEIVRQT
                     PNLYLSWISNFSCAPDSFMLHYLRWIMGRKPYLVLEIDSHTADAGLDTRIEAFLDIVD
                     SFRREAQPASPPHRGRRFRVDTATTPPAVVDERSGERLSLRDSRVTFVWPSFGDLASQ
                     ALAAATRRRGVRADHMPVPTARSTQLARNVASGKECIPALIVLGSILEYVASRPPGRP
                     DEVLLIGVPSTLGPCRTGQYHVFYDRLFDEIGLDHVALFVSGDDSSYGELGPDFSKDL
                     WRAILVSDYFTDIRSGIRICAADVDAGLAVFDRAWQDVVAALEGGPRDLAAALARAAA
                     ALGAVPRRRSLDEVKKVLVVGEIYVRRDTFSVHELSEYLIGQGIFPKLAGVSEWIHYT
                     NWARAYEMDAARARDGWLSALRPSALAERLGLGLETWWTKRAEAEVRAALEPAGLVPH
                     GLHDLGAAIEEGGRIFVSPEMQSEATVSSAVAGAAMRDGFAGIAIIAPFGCLPGRVIE
                     GVYAPWARARGYPVIALENDGQPYPPNVVSRIEVFAQNVERYERPAGS"
     gene            complement(4513..4902)
                     /locus_tag="PLT35_01675"
     CDS             complement(4513..4902)
                     /locus_tag="PLT35_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27962.1"
                     /translation="MRDLMKRHEAFVRGELATASADSDWRALARCHRRQVLDMQHERL
                     VHLLVTLFFGLCALVVFLFFVQHPDRVAVGVLLLLLIALLVPYIVHYFALENGVQRWY
                     ALADEIERRAGGVVARHDEGAIAPRPR"
     gene            complement(5028..6653)
                     /locus_tag="PLT35_01680"
     CDS             complement(5028..6653)
                     /locus_tag="PLT35_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG27963.1"
                     /translation="MELKRLSAGDLDLILKTLKEFTDREAPLDKRLKWDETDTCPEPV
                     VRAMLGPDVGLHLVFLPADHGGMGGGAYDVYRLSAEFAKIDLGLATAMLAVALGTDPI
                     RVGCTETQKAAWLPRIADEGLVVAYGVTEPEAGSNVQSLKTEAARITDADGRTTHYRL
                     NGVKQFISNGSIADLYTILAKAPDGPTFFVVERGTPGLSPGRHEIKHGIRLSNTAQVV
                     LQDVVVPAANLVGEREGEGLKQANEVFGFTRLMVAAFGLGAGQAALQKAVAYSTQRRQ
                     FGTFLAQKQGFTHKLLVPDSVRLAAAQAYIEHVADLLDSGNTDRQVEGSIAKLFATEA
                     ANAAADHAVQALGGYGYCVDFEVEKIRRDARILTIYEGTSEIQQSIIGLFRMRETVRS
                     KGAFYCGMADEIEAFADAGGPAAARAARFLAEATQAAFRAKAIRQQHVSFEFATAMAE
                     VETAVALARAAARAGASRLLKAQSRLWAAWVSLGVPVRLLAALNAAGSLGPDELARLR
                     QAGDFDAGLAAQSGGVADMNLVAEELTGFRVAE"
     gene            6850..8013
                     /locus_tag="PLT35_01685"
     CDS             6850..8013
                     /locus_tag="PLT35_01685"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG27964.1"
                     /translation="MPRAVVIGLIVLLAGAVGAAVPQAPSGQDGGQAIEPSHLGKKIC
                     GWPRPDDVPEEVWENACTACTSVPTSPEASADGTMTSHSCDGGYEIRIKVVPGRTYPA
                     GAKRDIMKGGGLGEEKPLQFQERKVGEIPQVERTFTRYDASYPFMNEKGVVIGETTIG
                     GRRELYSDEGLFDIMELERLALERASSAREAIRIMGEFAERYGYGDSGECLTVGDAAE
                     VWQFEIFGPGAADIGAVWAAKRIPPGEVGVSANVSRITTLGDDPNFTMHSKNVYEVAE
                     KNGWWKKGEPFVFNRAYGMSGSPRFNRREWRVLSLLAPSLKLDPWAPEQPFSVKPDRK
                     VTKQDLIAIHRDIYEGTPFDRRRTPWPARSARPTAGPSRATTSRSKATCRPSA"
     gene            7995..8591
                     /locus_tag="PLT35_01690"
     CDS             7995..8591
                     /locus_tag="PLT35_01690"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG27965.1"
                     /translation="MPTERMIAVHQCSYVVVLQARAGMPPWIGSLAWFAPDDAKTSVF
                     TPFYAGNLKVPPAFEIGRRDRFDRGSAWWASNFIGNWSNLNWRAIIQDVRAKQAEVEG
                     KLFADQPVIEAVALELYKTDPERARSFISDYSNRVAQENYMTWWAFADKLVTDYQDGG
                     ARVTPEKRTIPSDWLQKQGPIGTKVPPALVSPGAAPKK"
     gene            complement(8677..9342)
                     /locus_tag="PLT35_01695"
     CDS             complement(8677..9342)
                     /locus_tag="PLT35_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG27966.1"
                     /translation="MPRRPRLASGRYIFHVFNRAIQGLTLFESEADYEAFLRVLVETC
                     KCVPMRALAYVIMPNHWHFVLWPFEDCGLSDFMKRLSATHASRWRAARGNKGRGAVYQ
                     GRYKAIAVQHGAHFVRVCRYVERNPVRARLVARAEDWSWGSASPAAHSEGRPALAEWP
                     VQRPQTWLEMLNVPEPVRSLNAIRSAIATGRHYGTPSWRLLTSRELRWRSGLRMPGRP
                     KQA"
     gene            complement(9606..10436)
                     /locus_tag="PLT35_01700"
     CDS             complement(9606..10436)
                     /locus_tag="PLT35_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG27967.1"
                     /translation="MPMAEARRTGASASAGRAQRDYWNEIGRAWARGQRDASWRGCSD
                     AIHERWLGRVAADLQGGRVLKTDLFNEAIGDGFAGWFERRGCRMLACDVAWPTAARAA
                     ARHPSIAVVAADVRTLPLAPGAIDAVLSDSTLDHFACEAEIAASLRELRRTLRRGGTL
                     LLTMDNPRNPLIRLRNLCPGFWHRLGIVPYMVGVTCTRRRLEQMLDAAGFEVEASATI
                     MHAPRVAAVWLCRWWARATRRNRPAPWLVASLLAAEHLDRLPTRELTGHFVAIRARAR
                     "
     gene            complement(10460..11590)
                     /locus_tag="PLT35_01705"
     CDS             complement(10460..11590)
                     /locus_tag="PLT35_01705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOG27968.1"
                     /translation="MKRALPCLGALLLVAVAAAVYVQRSHRQMRDFEVYRVAGVRAAA
                     GQPLYRESDGHWMFKYLPAFALLVAPLAQLPSEGARALWFALTIGAAALFVRGSLALL
                     PARRRAPAFVVGITLLFMAKHFAREIGLGQSNALMAVTVLAALTAARGSRHAASGAWL
                     AAAAIIKPYALIFLPWLVVGRRWRAVAAYSAVAVAALAAPALRYGWAGNLAELRGWWT
                     SVTSSTAPNLTGQDTVSLAGMFAAWVGTGPAAAWLASALSLALVILCARVVLQGRDAS
                     SPDYLDAALLLFVIPLLSPQGWDYVLLLSTPAVMLLVDRLDEFRTPVRVLLVLSLAVA
                     GLSFWDVLGPEAYRALMMSRVVTLGALLQVWLVARLRARGAA"
     gene            11589..12428
                     /locus_tag="PLT35_01710"
     CDS             11589..12428
                     /locus_tag="PLT35_01710"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG27969.1"
                     /translation="MERTRTPYHGSGPARGTGFRDRALAGGTSLAAGADYVAAAMSRQ
                     VIAAGGADPFSAVERQLESLPSNRRAQSWKDVGMRTARGVIERTVRKGIEPRGQAAIM
                     FTGRFAFSPAERAALRAMSLVLETRLRATLREDSGGTDSVNAGHAKVPVPRYGLGISF
                     GCAADRTAELLKAVRAEIDLLRSSGPTPQQVSDAKIALVREFETNSRQNACLLNRISL
                     RHQHGEDLGQLLGLAGYDERAVTAAAIQQAARRRLDLDNCIQVTLLPEQVGQARSGLD
                     VQA"
     gene            complement(12447..12971)
                     /locus_tag="PLT35_01715"
     CDS             complement(12447..12971)
                     /locus_tag="PLT35_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27970.1"
                     /translation="MTIHPARLVPAAVLAFAMSACGGNGRSSAPPPATPAAPDHLVGL
                     HASDLTQALRERGLACQAPVLERDTRHWVCESSTPLVQYRAEFYAKAPGRIEYIRVVV
                     SQAGAARLEVAAPLLALIANQRYEGADPAKARAWIEANVATPGRIDIGPANLKLSGDL
                     SRLVLELKALGSDW"
     gene            13076..>13829
                     /locus_tag="PLT35_01720"
     CDS             13076..>13829
                     /locus_tag="PLT35_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27971.1"
                     /translation="MAVAALLLLSASALLTAQGRKPGVKAPSAQPPRVGPTDPALRLT
                     GFDAHRAKLASSPYRTLAWQFLGPKNVSGRTTDVAVIAPRGRSYTIYVATATGGLWKT
                     DNEGTTWRPVFDQGPSTTIGDVTAAPSDPDIVWMGTGEANIFRSSQAGAGVYKSVDAG
                     TTWQHMGLAGTYTIPRIVIHPTRPDTVYVAASGREWTDNPERGVYKTTDGGRTWAKVL
                     FVDDRTGAIDLVMDPSDPETLYAATWQGIRLKW"
BASE COUNT         2128 a         4840 c         4958 g         1903 t
ORIGIN      
        1 ggctggaggc gcacgtcctg aagctgtacc gggggcacgt gaagatcatt ccctccggcc
       61 tgccgggcgg gaacagcgcc gtgctcggcg cagccgccct gggctggacc gagctgctga
      121 aggcgcggtg agggcaagcg cctcaccccc tctcgcgcct gggcggcggt tcgccgcgaa
      181 gcgatccgcg ccggccttca cgagccggcg ggccgctcgt agcgctcgac gttctgcgcg
      241 aacacctcga tccgcgacac gacgttcggc ggatacggct gcccgtcgtt ctcgagggcg
      301 atgaccgggt acccgcgggc cctggcccac ggggcgtaga cgccctcgat gacgcggccg
      361 ggcagacagc cgaagggcgc gatgatggcg atcccggcga agccgtcgcg catggcggcg
      421 ccggccaccg ccgacgacac ggtggcctcg ctttgcatct cgggcgagac gaatatccgg
      481 ccgccctctt cgatggcggc gccgagatca tgcaggccgt gcggcacgag ccccgcgggc
      541 tcgagagcgg cgcgcacctc cgcctcggcg cgtttcgtcc accaggtttc gaggccgagc
      601 ccgaggcgct cggcgagggc cgacgggcgc agcgccgaga gccagccgtc cctggcgcgc
      661 gcggcgtcca tctcgtaggc ccgggcccag ttcgtgtagt ggatccactc gctgacgccc
      721 gcaagcttcg ggaagatgcc ctgcccgatg aggtactcgg acagctcgtg caccgagaac
      781 gtgtcacggc gcacgtagat ctccccgacg acgaggacct ttttcacctc gtcgagggag
      841 cgccgccggg gaacggcgcc gagggccgcc gccgcgcgcg ccagcgccgc cgccagatcg
      901 cgcggcccgc cctcgagggc cgcgacgacg tcttgccagg cccgatcgaa gacggccagg
      961 ccggcgtcca cgtcggccgc gcagatccgg atcccgctcc ggatgtccgt gaagtagtcc
     1021 gacacgagga tggcgcgcca gaggtccttc gagaagtccg gccccagctc gccgtaggac
     1081 gagtcgtccc cggacacgaa cagcgcgacg tggtcgaggc cgatttcgtc gaagagacgg
     1141 tcgtagaaaa cgtggtactg gcccgtccgg cacggtccca gggtcgatgg gacgccgatg
     1201 aggagcactt cgtccggccg gcctggcggc cgcgacgcga cgtactccag gatgctgccc
     1261 agcacgatga gcgccgggat gcactccttg ccggaggcga cgttccgggc gagctgcgtc
     1321 gagcgcgccg tgggcaccgg catgtgatcg gcgcgcacgc cccgcctgcg cgtcgccgcc
     1381 gcgagcgcct ggctggccag atcgccgaag gacggccaca cgaaggtgac cctcgaatcg
     1441 cgcaggctga ggcgctcccc gctgcgctcg tccacgacgg ccgggggcgt cgtcgcggta
     1501 tcgacgcgaa agcggcggcc gcggtgcggc ggggacgcag gctgtgcctc gcgcctgaag
     1561 ctgtccacga tgtccaggaa cgcctcgatg cgcgtgtcca ggcccgcgtc ggccgtgtgc
     1621 gagtcgatct cgaggacgag gtacggcttg cgccccatga tccagcgcag gtagtgcagc
     1681 atgaacgagt cgggcgcgca cgagaagttc gagatccacg acagatagag gttcggcgtc
     1741 tgccggacga tctccgccgc cttcatgtcc tgctgcccgt agtaccagta catgttgggg
     1801 aagattccgg ccgactcgtc gtagatcatg tcgaagggca cgaccgtcac gccgcgggac
     1861 gtgaacttgc gggggatgcc catgttcgcg tcgcgggtga acgcgttgta gggccgcccg
     1921 aacaggccga cgtacaggcg atcgggaagg ctgcggatct cgtccatcac ctcgcgcccg
     1981 cgctgccggt agcgctcgag gaaggcgcgg tagctcccga cggcctcgtc gaacgcctgg
     2041 ccgccctcgg ccgcgtcgaa gcccagctgc gccgccacgc gctcgaactc cggccggcac
     2101 gcctcccacc cggccttgaa cgacacgacc ggccgcagca cggtcgcgtc cacgccgaac
     2161 gcctggcggg ccaggtacgg cagcccctgc gtcagcgggc aggtgcacgc gtggaccttc
     2221 ccggactcca tcgacggcat gtcgcggaag tgcggcagga acaggaagtc cgcccggcgg
     2281 tcgaggaggt cctggattgc gccgtgggcg atctcgacgg ggaagcagta gctgctttcc
     2341 tggcgggcca cgccttccgg cgcgatccgc tccgacagca ccgcccggac gcccagctcg
     2401 tggaagaagc gggcgtacag cggccagagt gagtgcaccg acagcgcgcg cggcacgccc
     2461 accacgcggt cggtgcgcgg gcggaagcgg gccgggtccg gcgcggcgcg ctcgaacact
     2521 tcgtcgtggc gccaggccac gtgatcgacg gcgtcccgct ccgacccggg cagcctccgc
     2581 cgcgccccgg tgaacttcga gcagcgtccg ccgaacggat aacggtgacc gtccaccgac
     2641 aggttccgga tcgggcaccg gttgtcgcag gcctggcagg tgaactcgcc gagcgtctcg
     2701 atctccttcg cggcgagcgt gcgcaggtcg aaggagcccc gggcgaggag gcccgcgcgg
     2761 tgcttctcga gggccaggcg ggcgacgccg aagcagccca tcagctcggg gtccggcggc
     2821 accgagacgg gcttgccgac gagctgcgcg aacgccagcg ggacggcggg gttcttggcc
     2881 acgcccccct gcagcgcaat ccgctcgccc acggaccggt tcccgacgac gcggttcagg
     2941 tagttggcga cgatcgagaa cacgaggccg gcgacgatgt cggcccggtc cgagccgttc
     3001 tggatggcct tgcggatgtc gctgttgatg aaggccgagc agtgctcgcc gaacttgagg
     3061 ggcgccctgg cctccagcgc gatggggccg atgtcctcgg cgcgctccac gcagaggtcg
     3121 ccggcggccg attcctccag gaacgagccc gtgccggccg agcaggcctc gttcatcgcg
     3181 tagtcgatcg gcacgccgtt gttgagcagc acgtacttcg cgtcctggcc gccgatctcg
     3241 aagagcgtgt ccacgtccgg ttcgaagtac gtcgtgcccg ccgcgtgcgc gatgatctcg
     3301 ttgtagacgc cctcggtctc gaggaagacg cccagcagct cccgggacga cccggtggcg
     3361 gcggccagcg tgatcgccgg ccgggcgccg cccagttggc cgtccacttc gcgcaggcag
     3421 cgcctgagcg ccgccacggg gtcgccgtgc gtgcgcccgt agtgggccgc ggcgatttcc
     3481 agcgtctccg cgtcgatgag cgccaccttg gtcgtcgtcg atccgccatc gacgccgagg
     3541 atgtactccg cgcccggccg caacgcgcct cgcttcgacg ggtagtaggt gacgcgcgag
     3601 gcggccgagg ccagcggctc gaacggcgtg ccctccgcgg cggccagggc gcgcagcctc
     3661 cgcctcgcag gcggcagcgg ccggccgagc gaggccgcca ggtgcgcggc gccgaaggcc
     3721 tcgaagtagc gcgcctccgc cggcatgatg aagcgcgtcc gcggccaggc ctcgcgcagg
     3781 aaccgcacga agtgcgcgtt ctcggtgacg ccgccgacga gcacgacctc gcccgcctgc
     3841 accttggccc ggatgaggaa ctcgccgacc ttgtcggcca tcaccttcga cagcgagagc
     3901 gcgatgtcgc ccttggtcgc ctcgcccttg ttcaggcggt gcgtgcagtc cgacttcatg
     3961 aagaccgagc agcgcgccga cagcttgagg acgcgcgcgc tggcggccgc ctggtcgaga
     4021 tcctccagcc gaaggtccat gcgcccgagc tgctggcgga agaactcgcc ggttcccgag
     4081 gcgcacttgt tgcccgcgta cgtcgtctgg acctgcgcgc gcgcgttcag gatgtagacg
     4141 acgagatctt cgccgccgac ggacacgacg gcgcgcggac ggagcgaaag ggccccgagc
     4201 gccgcctcga tcgccagcgg ggcgatgacg tccggcgcgt cgatccggcg cctgcccgcc
     4261 ccgcccgtgg cgaccgtcgc cggcccgacc ggtccgccgg cgccgatcgc gctcagcgcc
     4321 tgctcgagcg ccgtttcgag atcgccgtcg tgcggcacga ccgcgtgcgc ggccccgctc
     4381 cggccgtcga ggccgcacac cttgacgttc accgacccga tgtcgatgcc gagggccgcg
     4441 tagccgcaca gggcgccgag ggcatccgcc tgcgtcaccc cggcggcctc cgctgcggac
     4501 gcgctgccgg gctcaacggg ggcggggcgc aatggctcct tcgtcatgac gggcgacgac
     4561 tcctccagcc cggcgctcga tctcgtccgc cagggcgtac cagcgctgca cgccgttctc
     4621 gagggcgaag tagtggacga tgtacgggac gagcagtgcg atgagcagca gaagcagcac
     4681 gccgaccgcg acgcggtcgg ggtgctgcac gaagaacagg aagacgacca gcgcgcagag
     4741 gccgaagaag agcgtgacga gcaggtggac gaggcgctcg tgctgcatgt cgagcacctg
     4801 ccggcggtgg cagcgggcga gggcgcgcca gtcggagtcg gcggacgccg tcgcgagctc
     4861 gccgcggacg aacgcctcgt gccgcttcat caagtccctc atgccagcct ctccgccacg
     4921 ccagcggcgt gcggcgcagc gtgttcccgc cgcccgtccg cctgcgcgaa tggctcgcgc
     4981 cagggcccga agcgggcccg gcgctgcctg cgtcgcgtcg gtgaaaccta ctcggcgacc
     5041 cggaagcccg tgagctcttc cgccacgagg ttcatgtcgg ccacgccgcc cgactgagcg
     5101 gcaaggccgg cgtcgaagtc cccggcctgc ctgagccggg cgagttcgtc cggtccgagg
     5161 ctgcccgccg cgttcagcgc cgcgaggagc cgcactggca cgcccaggga cacccaggcg
     5221 gcccagagcc gcgactgcgc cttcagcagg cggctcgcgc cggcccgcgc ggccgcgcgc
     5281 gccagcgcga cggccgtttc cacctccgcc atcgccgtcg cgaactcgaa cgagacgtgc
     5341 tgctgccgga tggccttggc gcggaaggcc gcctgcgtgg cctcggcgag gaagcgcgcg
     5401 gcgcgcgccg ccgctggccc gcctgcgtcc gcgaaggcct cgatctcgtc ggccatgccg
     5461 cagtagaacg cgcccttgct cctgaccgtc tcccgcatgc ggaagaggcc gatgatgctt
     5521 tgctggatct ccgaggtgcc ctcgtagatg gtcaggatgc gggcgtcgcg ccggatcttc
     5581 tcgacctcga agtccacgca gtaaccgtac ccgcccagcg cctgcacggc gtggtcggcc
     5641 gcggcattgg ccgcctccgt cgcgaagagc ttcgcgatcg agccttcgac ctgacgatcg
     5701 gtgttcccgc tgtcgagcag gtccgccacg tgctcgatgt acgcctgcgc ggccgcgagc
     5761 cgcacgctgt ccggcaccag cagcttgtgg gtgaacccct gcttctgggc caggaacgtc
     5821 ccgaactgcc tgcgctgcgt cgaataggcg acggccttct gcagggcggc ctggcccgcg
     5881 cccaggccga acgccgccac catcagccgc gtgaagccga acacctcatt ggcctgcttc
     5941 aggccctcgc cctcgcgctc gccgacgagg ttggcggccg gaacgacgac gtcctggagc
     6001 accacctgcg ccgtgttcga gagccggatg ccgtgcttga tctcgtggcg gcccgggctc
     6061 agccccggcg tgccgcgctc gaccacgaaa aaggtgggcc cgtccggcgc cttcgcgagg
     6121 atcgtgtaaa ggtccgcgat cgagccgttc gagatgaact gcttgacgcc gttcaggcgg
     6181 tagtgggtcg tcctgccgtc cgcgtcggtg atccgcgccg cctcggtctt gaggctctgg
     6241 acgttcgacc cggcttccgg ctccgtgacg ccgtaggcga ccacgaggcc ctcgtcggcg
     6301 atccgcggca gccaggccgc cttctgcgtc tcagtgcagc ccacgcggat cgggtcggtg
     6361 cccagtgcga cggccagcat ggcggtggcc aggccgagat cgatcttcgc gaactccgcc
     6421 gacaggcggt agacgtcgta ggcgccgccg cccatcccgc cgtggtcggc gggcaggaac
     6481 accaggtgga ggccgacatc cggcccgagc atggcgcgca cgacgggctc gggacaggtg
     6541 tcggtctcgt cccacttcag gcgcttgtcg agcggggcct cgcgatcggt gaactccttg
     6601 agggtcttga ggatgaggtc gaggtccccg gcgctgagtc gcttcagttc catgcccgct
     6661 cccggcggtg ccgcagccca cagcggcgcc ttccttcagc ccattcttcc gaaaaccccg
     6721 ccggggcgca agcggcggcg gccggcccga ctcggccggc gcgcagccct gcctctgcct
     6781 ggcccttcgt gatacgcttc gctgtcgagg ccggggggtg cccggccgcc aaggagagtc
     6841 gccgtgccga tgccaagagc cgtcgtgatt gggctgatcg tgctgctcgc cggagcggtg
     6901 ggagcagcgg ttccgcaggc accttccggg caggacgggg ggcaggcgat cgagccgtcc
     6961 catctgggca agaagatctg cggttggccc aggccggacg acgtgcccga ggaagtatgg
     7021 gagaacgcct gcacggcctg cacgtccgtg ccgacgagcc cggaggcgtc tgccgacggc
     7081 acgatgacga gccactcgtg cgacggcggc tacgagattc gcatcaaggt cgtgccgggc
     7141 cggacctacc cggccggcgc caagcgggac atcatgaagg gcggcggcct gggcgaggag
     7201 aagccgctcc agttccagga gcgcaaggtc ggggagatcc cgcaggtcga gagaacgttc
     7261 acccgctacg acgcctccta cccgttcatg aacgagaagg gcgtcgtcat cggcgagacc
     7321 acgattggcg ggcgccgcga gctgtacagc gacgaggggc tcttcgacat catggagctc
     7381 gagcggctcg ccctcgagcg cgcgtcgtcg gcgcgcgagg cgatccggat catgggcgag
     7441 ttcgccgagc gatacggcta cggcgacagc ggcgagtgcc tgacggtcgg cgacgcggcc
     7501 gaggtgtggc agttcgagat cttcgggccg ggcgccgccg acatcggggc cgtgtgggcc
     7561 gccaagcgca tcccgccggg tgaggtgggg gtgtcggcga acgtgtcccg catcaccacg
     7621 ctcggagacg acccgaactt cacgatgcac tcgaagaacg tgtacgaggt cgccgagaag
     7681 aacgggtggt ggaagaaggg tgagccgttc gtcttcaacc gcgcgtacgg gatgtcgggc
     7741 tccccgcgct tcaaccgccg ggagtggcgc gtgctgagcc tgctggcgcc gtcgctgaag
     7801 ctcgacccgt gggcgcccga gcagccgttc tcggtcaagc ctgaccggaa ggttaccaag
     7861 caggacctca tcgccatcca ccgggacatt tacgagggga cgccgtttga cagacgacga
     7921 accccctggc cggcccgttc ggctcgccca accgctgggc cctcgcgcgc gactacaagc
     7981 cggtcgaagg ctacatgccg accgagcgca tgatcgccgt tcaccagtgc tcctacgtcg
     8041 tggtcttgca ggctcgggcc ggaatgccgc cctggattgg cagtctcgcg tggttcgctc
     8101 ccgacgatgc gaagacgtcc gtgttcacgc cgttctatgc cggcaacctc aaggtgcccc
     8161 cggcgttcga gatcggccgc cgagatcggt tcgaccgcgg ctccgcctgg tgggcgtcga
     8221 acttcatcgg caactggtcg aacctgaact ggcgcgccat catccaggac gtccgcgcca
     8281 agcaggcgga ggtggaaggc aagctgtttg ccgaccagcc cgtcatcgag gccgtcgcgc
     8341 tggagttgta caagaccgac ccggagcggg cgcggtcgtt catcagcgac tactcgaacc
     8401 gcgtcgcaca ggagaactac atgacgtggt gggctttcgc cgacaagctc gtcacggact
     8461 accaggacgg cggggcgcgc gtgacccccg agaagcggac gatcccgtcc gactggctgc
     8521 agaagcaggg ccccatcggg acgaaggtgc cgccggcgct cgtttcgccc ggcgcagcgc
     8581 cgaagaagta agcgaagggg tcgggacggt ttccgtaccg ctgaggtgtt ggattacgaa
     8641 atcgtgaggc agagccggcc ggtagggagt gagagtctac gcctgcttgg gccgtcctgg
     8701 catcctgagg ccgctgcgcc agcggagttc ccgcgaggtc agcaggcgcc aggacggcgt
     8761 gccgtagtgc cgcccggtgg cgatcgccga tcgtattgcg ttgagtgaac ggactggctc
     8821 aggcacattc agcatttcaa gccaggtctg cgggcgttgc acgggccatt ccgccaaggc
     8881 cgggcgaccc tcgctgtggg ctgcaggcga agcgctgccc cagctccagt cctcggcacg
     8941 agccaccagg cgtgcacgaa ccggattgcg ctcgacgtag cggcataccc gcacgaaatg
     9001 ggcgccgtgc tgcacggcga tcgccttgta gcgtccctgg tatacggccc ctctcccttt
     9061 gttgcccctg gcggcgcgcc agcgcgaagc gtgtgtggcc gacaaccgct tcatgaagtc
     9121 cgacaaaccg caatcctcaa acggccatag cacgaagtgc cagtgattcg gcatgatgac
     9181 gtaggcaagc gcccgcatcg ggacgcactt gcaggtttcg accagtacgc gaagaaacgc
     9241 ctcgtagtcc gcttcgctct cgaagagcgt aagtccctga atcgcgcggt tgaagacgtg
     9301 gaagatgtat cgtccgcttg caagccgggg tcgtcttggc acctcaccct cctggattgg
     9361 ccaggattgt tgcaacgcgc ctgccgggtg gacgcgtcgc ctcatgccga ggaaccagtg
     9421 caatcacgga ggtgccgatc atgtccgagg cggcggccgc agaaatcgca atggttgggc
     9481 cgtggaggca tgcgataggc tgccaaccgt gggccggatc ggtcggacgg aatcaaggat
     9541 ggtcgcgatt acgtttctgt tgcctggagg tgcacaccct acgtaattcg tcccgacccc
     9601 tttgtttagc gcgcccgggc gcggatggcg acgaagtggc cggtcaattc ccgcgtcggc
     9661 aagcggtcga ggtgctcggc ggcgagcagg ctcgcgacga gccagggcgc gggccggttg
     9721 cggcgcgtag cgcgcgccca ccatcggcac agccacacgg ctgcgacgcg gggcgcgtgc
     9781 atgatcgtcg cgctggcttc gacctcgaat ccagccgcgt cgagcatttg ctccagccga
     9841 cgccgcgtgc acgtcacgcc taccatgtag ggcacgatgc cgaggcgatg ccagaagccc
     9901 ggacacaggt tccgcagccg aatcagcgga ttcctcgggt tgtccatcgt caggaggagc
     9961 gtcccgcccc ggcgcagcgt ccggcgcagt tcgcggagcg aggcggcgat ctccgcctcg
    10021 catgcgaagt ggtcgagcgt cgagtccgag agcactgcat cgattgcgcc gggcgcgagc
    10081 gggagggttc tcacatcggc ggcgacgacg gcgatggacg gatgccgcgc cgcggcgcgc
    10141 gccgccgtgg gccaggccac atcgcaggcc agcatgcggc acccgcgccg ctcgaaccac
    10201 ccggcgaacc cgtcgccgat ggcctcgttg aagagatccg tcttgagcac gcgcccgccc
    10261 tggagatccg cggccacgcg gccgagccat cgctcgtgga tcgcatcgct gcaccctcgc
    10321 caggacgcgt cgcgctggcc gcgggcccat gcccggccga tctcgttcca gtagtcgcgc
    10381 tgcgcgcgcc cggccgaggc cgacgcgccg gtccggcggg cctcggccat cggcatgaac
    10441 cccgcccttc cgcaacctgc taggccgcgc cccgcgcgcg gagccgggcg acgagccaga
    10501 cctggagcaa agcgccgagc gtcacgacgc gggacatcat cagcgcccgg taagcctcgg
    10561 ggccgagcac gtcccagaag gacagcccag caaccgcgag cgagaggacg agcagcacgc
    10621 gcacgggcgt gcggaactcg tcgagccgat cgacgagcag catcaccgcg ggcgtcgaca
    10681 gcaggagcac gtagtcccag ccctgcggcg acagcagcgg gatgacgaag agcagcagcg
    10741 ccgcgtcgag gtagtccggc gacgacgcgt cgcgtccctg caggacgacc cgtgcgcaga
    10801 ggatcacgag ggcgagcgac agcgcggaag cgagccacgc ggcggctggg ccggtgccga
    10861 cccacgcggc gaacatgccg gcgagcgaca cggtgtcctg gcccgtgagg ttcggcgcgg
    10921 tcgaggaggt cacgctcgtc caccaccccc ggagttcagc caggttcccg gcccagccgt
    10981 agcgcagggc cggggcggcg agcgcggcga cggcgacggc cgaatacgcc gccacggcgc
    11041 gccagcgccg gccgacgacg agccagggca ggaagatcag ggcatagggc ttgatgatcg
    11101 ccgcggccgc gagccaagcg cccgacgccg catggcggct gccgcgcgcg gccgtcagcg
    11161 cggccagcac ggtcacggcc atcagcgcgt tgctttggcc gagtccgatc tcgcgggcga
    11221 agtgcttcgc catgaagagc agggtgatcc ccacgacgaa cgccggagcg cgcctgcggg
    11281 ccggcagcag cgccagggat ccgcggacga agagcgcggc cgctccgatg gtgagggcga
    11341 accagagcgc gcgggcccct tcggacggca gctgcgcgag gggcgcgaca agcagcgcga
    11401 aggccggcag gtacttgaac atccagtgcc cgtccgactc gcggtagagc ggctggcccg
    11461 ctgcagcgcg cacaccggca acgcggtaca cctcgaaatc gcgcatctgg cggtgggacc
    11521 gctggacgta caccgcggcg gcgacggcga caagcagcag ggctcccaga cacggcaggg
    11581 ctcgcttcat ggagcggacg cggacaccat atcacggcag cgggccggct cgcggcacgg
    11641 ggttccggga ccgcgctctg gcgggcggca cgtcgctggc ggccggcgcg gactacgtgg
    11701 cggccgcgat gtcgcgccag gtgatcgccg ccggcggggc cgacccgttc agcgccgtcg
    11761 agcgccagct cgagtcgctg ccgtcgaacc ggcgcgccca gtcgtggaag gacgtcggca
    11821 tgcggaccgc ccgcggcgtt atcgagcgga ccgtgcgcaa gggcatcgag cccaggggcc
    11881 aagcggcgat catgttcacc ggacgcttcg ccttctcgcc ggcggaacgc gcagccctgc
    11941 gcgcgatgag cctcgtgctc gagacgcggc tgcgcgcgac gctccgcgag gattcaggcg
    12001 gcacggacag cgtcaacgcg ggccacgcga aggtgccggt gccgcgctac ggtctcggca
    12061 tctcgttcgg gtgcgcggcg gacaggacgg ccgagctgct gaaggccgtg cgcgcggaga
    12121 tcgatctgct gcgctcgtcc gggccgacgc cgcagcaggt gagcgacgcc aagatcgcgc
    12181 tcgtgcgcga gttcgagacg aactcgaggc agaacgcctg cctgctcaac cggatctcgc
    12241 tcaggcacca gcatggagag gatctgggcc agttgctcgg cctcgccggc tacgacgagc
    12301 gggccgtcac cgctgctgcg atccagcagg ccgctcggcg ccgcctcgat ctcgacaact
    12361 gcatccaggt gacgctgctt cccgagcagg tggggcaggc gcggtcaggt ctcgatgtcc
    12421 aagcctgacc ccgcctggtc tggaggtcac cagtccgatc ccagcgcttt gagctccagc
    12481 accaggcgcg agagatcgcc ggagagcttc aggttcgcgg ggccgatgtc gatcctgccg
    12541 ggcgtcgcga cgttcgcctc gatccacgcc cgcgccttcg ccgggtccgc cccttcgtac
    12601 ctctggttcg cgatcagcgc caggagcggc gccgccacct ccagcctggc cgcgccggcc
    12661 tgggagacca ccacgcggat gtactcgatc cgtccgggag ccttcgcgta gaactcggcg
    12721 cggtactgca ccagcggcgt ggacgattcg cacacccagt gccgcgtgtc gcgctcgagg
    12781 accggcgcct ggcaggccag tccgcgctcg cgcagggcct gcgtcaggtc gctcgcgtgc
    12841 aggccgacca ggtggtccgg cgcggcggga gtcgcggggg gcggcgcgga ggaccggccg
    12901 ttgccgccgc acgcggacat cgcgaaggcc aggaccgccg ccgggacgag ccgtgcaggg
    12961 tggattgtca tggcgcggat tctatagcat gtgggaggcg cgacagacgc atcgaggagc
    13021 acgggcaatg cagagcggca ggcaggcacc ttcgtgcaga ccgggaatca tcgggatggc
    13081 cgtagcggcg cttctcctgc tgtcggcgtc cgccctgctg accgcgcagg gccgcaagcc
    13141 gggcgtgaag gcgccctccg cgcagccgcc gcgcgtgggg cccacggacc cggcgctccg
    13201 cctgacgggc ttcgacgcgc accgggcgaa gctggcgtcg tcgccctaca ggaccctggc
    13261 gtggcagttc ctcgggccga agaacgtcag cggccggacg accgacgtcg cggtcatcgc
    13321 cccgcgcggc cggtcttaca cgatctacgt cgcgacggcg accggcggcc tctggaagac
    13381 cgacaacgaa ggcacgacgt ggcggcccgt cttcgaccag gggccctcga cgacgatcgg
    13441 cgacgtcacc gccgcgccgt cggatccgga catcgtctgg atgggcaccg gcgaggcgaa
    13501 catcttcagg agttcgcagg cgggcgcagg cgtctacaag tccgtcgacg ccggcacgac
    13561 gtggcagcac atgggcctgg ccggcacgta cacgatcccg cggatcgtca tccatccgac
    13621 ccgtcccgac acggtgtacg tggcggcgtc gggccgcgag tggacggaca accccgagcg
    13681 cggcgtctac aagacgaccg acggcggccg cacgtgggcg aaggtcctgt tcgtcgacga
    13741 ccggaccggg gcgatcgacc tcgtgatgga cccgtccgat cccgagacgc tgtacgccgc
    13801 cacgtggcag ggaatccggt tgaagtgga
//
LOCUS       DAOSIF010000023        13733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4562_length_13733_cov_3.786372, whole genome shotgun sequence.
ACCESSION   DAOSIF010000023 DAOSIF010000000
VERSION     DAOSIF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4562_length_13733_cov_3.786372"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLT35_01725"
     CDS             <1..215
                     /locus_tag="PLT35_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27972.1"
                     /translation="KDTAGPAVNPLIKVMNMVSLLTLGLVLQYNLLDPRGGAHVRFVG
                     ILVALACAVSVGWAVWKSKQETEPVS"
     gene            225..1460
                     /locus_tag="PLT35_01730"
     CDS             225..1460
                     /locus_tag="PLT35_01730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG27973.1"
                     /translation="MSTLRHPPRRRRPDPSGRRRVARRPAAASPAALPFEAAPHDATP
                     IEFAALPLDDRLRAGIADRGFVRTTPVQSAVFPLVFSGADLIACAETGTGKTAAFLLP
                     IMQRLLERPAAGATRVLILEPTRELAVQIEDDFTGFSYYTDLTGAAVYGGVDGDSQAH
                     ALRAGADVIVATPGRLLDHMNCGVADFTRVEVLVLDEADRMLDMGFWPDVRRIVSALP
                     GARQTLLFSATTSSDVVALAEQVMRDPRFIQIGRAGGPARTITHIAHVVPAREKVEWL
                     TRFLRRSIGPSLVFVRTKRGADTLASALAGRGIRCAALHADRTQRDRSAAVEGFRAGR
                     HSALVATDIAARGLDIDGVAHVVNYDVPDTAETYVHRVGRTGRAEAAGTAITLVAPDE
                     LPALQAIERSIDIYIDRPA"
     gene            1508..2497
                     /locus_tag="PLT35_01735"
     CDS             1508..2497
                     /locus_tag="PLT35_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG27974.1"
                     /translation="MQTRQLGTTDLHLTPIGFGAWAIGGPDYAFGWGPQEDSESIAAI
                     HRAIDAGINWIDTAAVYGLGRSERVVAKALAQVGPSRRPFVFTKGSLVWNESGAITHA
                     LAPASLRKEVDDSLRRLGTDVIDLYQIHWPEPFGGNGSAPEIEEAWRTLLDLRQAGKI
                     RHLGVSNFDEVQLARVHAIAPVASLQPPYSMLRRGIERAVLPYCLDNRIGVIVYSPMA
                     AGALSGTMTRERAASLPKNDWRSQHADFREPKLSRNLALVEVLRGIGSRHGVSPGVVA
                     VAWTLRHPAVTAAIVGLRSPSQVDGIAPAGELRLTAEEIAEIEPHVQAAPRSF"
     gene            complement(2507..3472)
                     /locus_tag="PLT35_01740"
     CDS             complement(2507..3472)
                     /locus_tag="PLT35_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOG27975.1"
                     /translation="MMAPGAQTIARRLASIDVLAMAFLSGLGAIALARLGDIPAWPTV
                     AATCAAIVIGVPLLAWVRARLDLRAVRVLHDWSFAVYLLAIYRAVLLVNGPLHGGRLY
                     DGWLIASDRWIFGVDPTVWIARFAHPIATEIFQVAYWSYYVIPLAVGAEFYLRGREWP
                     FRQWLFVCGFGFFVTYVGYLLVPAISPRFTLHDPGSAARELPGLWLTPHLRAMIDAGG
                     MAPVDATPAEAMRLAPRGAFPSGHSLVTLLTVAWAWRRRLRVRWFVTVAGALLLVATV
                     YLRYHYVTDLIAGALLASVCYAAAPALHGRVSRRLGTLDAQAEGR"
     gene            complement(3469..4587)
                     /locus_tag="PLT35_01745"
     CDS             complement(3469..4587)
                     /locus_tag="PLT35_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27976.1"
                     /translation="MVEIKDVRTRLDLRRFIAFPERLYRGHPYYVPKLIGEEKKALRP
                     DRNPAFEYCEAKYWMAFRDGEAAGRVAGIVSHRYNETWQKRRARFGWLDFTDDAEVSA
                     ALLGAVEDWARSRGLAEVHGPLGFCDLDRQGLLVEGFEELDLLITNYNYPYYASHLER
                     LGYRKDADWVELQLPVPPVMPEALERIAKAALERTKVRVLQTRSMKDILPYVERIFEA
                     VNETYRGLYGVVRLSDAQVQYYTKEFFGVLDHRFVTIILDRDDNIAAFGAVMPSLARA
                     LQQCRGRLFPFGFLRILRDLRTSDRLEMLLTAVRPPYQKKGLNAVLIHEAWKSAVARR
                     IKLAETGPMLESNTQILAQWKGLESRQHRRRRCYVKAL"
     gene            complement(4692..5468)
                     /locus_tag="PLT35_01750"
     CDS             complement(4692..5468)
                     /locus_tag="PLT35_01750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG27977.1"
                     /translation="MADERESSGHGAFERWMDGLEARHLGDLRFPEVSRAVRALSSAY
                     VERRGRLASGAALDGAGKRAAFALFYGPLHFLLVRAIVRALGAARPAARVVGDLGCGT
                     GAAGAAWALECAPRARVAGIERHPWAAGEARRSLRALGLDGHVRCADLAHAGAPPRAD
                     AIVAAFTLNELGEEARVRCLRALLGAHARGARVLVVEPLAAAVAPWWPAWASAAEAAG
                     GRQDQWRFPAALPDLLARLDRAAGLDHRELTARSVYLSVR"
     gene            5927..6904
                     /locus_tag="PLT35_01755"
     CDS             5927..6904
                     /locus_tag="PLT35_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOG27978.1"
                     /translation="MTLNDVRDAAERIATIARLTPVVPAAEDSGGPDLLLKCENLQRG
                     GAFKIRGAANMILQLPDAECRRGVITYSSGNHGIAVSLAAQLAGVPAIVVMPTAAPAV
                     KTAAVRRLGAEIVFEGTTTVERKARAEVEAAARGLTIVPPFDHRWIIAGQGTIGLELF
                     AQAPEAAAVYVPVSGGGLVSGIAAALKALSPAVRVIGVEPAAVPRIARSLAAGGPITV
                     PASSGLADGLLAVRPGDATFAHVRAYVDEIVTVSDEAIARAVSWVFERAHLVAEPSGA
                     ASVAAALGWAEPAWSRPGAGPALPSPGPKVAVVSGGNVETDTFIRCIRQ"
     gene            complement(7984..9225)
                     /locus_tag="PLT35_01760"
     CDS             complement(7984..9225)
                     /locus_tag="PLT35_01760"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOG27979.1"
                     /translation="MRGTPTTFWGKLTKPSRAEEVLEWHPLVDHCADVAAVAEALLTL
                     PVWQARFNALAGEPLDFGQRARLAVLAALHDLGKFNIGFQANGRDGLGVPAGHVSEAI
                     AVLNGVLGTWLAALGDWGDGVSGLLLAALGHHGRPITFEQAAQCWRPEVWLPRADLDP
                     EAGVADLLARCRAWFPPAFARRTRDLPDRAELSHAFAGLVMLADWIGSDSAYFPFSDD
                     ATADRMERSRCYAQRALRGIGLHYPACDRTDPQQRDAYARVAPEWAGQPRPIQSAVVQ
                     LPIAMSGTITVVESETGSGKTEAALSRFVQLFEAGLVDGMYFALPTRTAATQLHERVR
                     RAIQQAFRDPPPVVLAVPGYCRVDDVEGGQRRLAFFRSVVAGCRPASLPRLGSGEPET
                     FSRWSRFGWHGFPRVRGDAPR"
     gene            complement(9452..10777)
                     /locus_tag="PLT35_01765"
     CDS             complement(9452..10777)
                     /locus_tag="PLT35_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG27980.1"
                     /translation="MAIDWSDIRYRGDAKDVEELLTTYRVADYLATFEENRRQIDRGV
                     RERLLKEGIRLSERLSPRIYRVFNDVTAALGLETEAEVFCLPSTEINAFAILDVRKDT
                     TYSLVGVTAPALERLDDAELRSILGHELGHILFGNNRLAALISTDASNPSVTVLPALG
                     ESLYLRWRKKAEISADRVSVLASRDFRATATSLLKATFGLSERNLNLDIEALVNQVDE
                     IRGKPELMEEVFASHPLLPIRLKAVELFSRSAKAARAGYPVEGEPIADGALEDAVDDL
                     VRLTRRYPAQPLHIAVMRAVALGGAQLLAADRDVSDEEVKILVQILHKWFTDEPEAEI
                     ETSREAIAARLPDALDTVRKDGSLDDKVFILTRLMDIALADGALMDPESSIILDVAKH
                     LDVPSKIAYSVIVGSTQSIGFRTDVKLNRMAEAIRRAMKMELKGERAKK"
     gene            complement(10821..12761)
                     /locus_tag="PLT35_01770"
     CDS             complement(10821..12761)
                     /locus_tag="PLT35_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG27981.1"
                     /translation="MLLESVRVENFKCIDDSTEFSIRRLTALVGKNESGKTALLKALY
                     RVNPILPVDGDFVETEYPRRRWAEYRKRRGQSPDRVVTATWRLEGPDAAAVAEAAGPG
                     ALPEPLVSVSRGYDNRLRWHIDVDERAVVAGLLGRAGLDAGERERVSGAASVAELVAL
                     LRGLEAPTPALAALLVDVEARFPDASPASAIAAALEPRLPRFLYFAEYHRLPGEVSID
                     DLMRRKAAGALTVVDTIFLALLELAHTTPEELAGIDQFEYLVAELESVSQRISRDVME
                     YWSQDRALEVDFRCDAARPHDPAPFDTGYILRMRIRNRRHRVTLGFDERSTGFIWFFS
                     FLVWLSHLRRDHGADYVILLDEPGLGLHARAQMDMLRFIREQLLPAHQVIYTTHSPFM
                     IDGDYLDGLRTVEDVSDGDSVVGTKVGDRALSTEPDTLFPIRAALAYDVARRVVTGPQ
                     TLLVDRASDVLYLKWFSRELQLQGRAHLSPAWSLVPIGGLDSIAPFVSLFAGQAQRIA
                     VLTDGLMSHPRRLDGLWERGLLQFGRILRPAFYVDLEDAQVEDLLGRRLFRELVTGAY
                     GLEESAVLDEGESAAARRVVAEVEAYFASMPPWAPRFDSFAPASYLFERTSTLRSTLP
                     ELGAALDRFEKLFRDLHSLAIQ"
     gene            12921..13403
                     /locus_tag="PLT35_01775"
     CDS             12921..13403
                     /locus_tag="PLT35_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOG27982.1"
                     /translation="MDLQLVPVAMPENANVVIGQSHFIKTVEDLYEIMATTVPGAAFG
                     LAFNEASGACLVRSDGNDEELRAAAVDAALALGAGHTFVLYMRGAYPINVLNRIKDCQ
                     EVCRVFCATANPLQAVVAESDQGRGIVGVIDGSSPKGVEAEADREWRAGFLRRIGYKR
                     "
BASE COUNT         2048 a         4958 c         4809 g         1918 t
ORIGIN      
        1 tcaaggacac cgccggcccg gccgtcaacc cgctcatcaa ggtgatgaac atggtgtcgc
       61 tgctgacgct cgggctggtg ctgcagtaca acctgctcga tccgcggggc ggggcgcatg
      121 tccggttcgt cggcattctg gtcgcgctgg cctgcgcggt gtccgtcggc tgggcggtgt
      181 ggaagagcaa gcaggagacc gagcccgtgt cataatgggg cgttttgagt acccttcgcc
      241 atcctcccag gaggcgccgg ccggatccgt ccggccggcg ccgcgttgcg cgccggcccg
      301 ccgccgcttc gccggcggcg ctgcccttcg aggcggcgcc ccacgacgcc acccccatcg
      361 agttcgccgc gctgcccctc gatgaccgcc tgcgcgccgg catcgccgac cgcggcttcg
      421 tccgcaccac gcccgtccag agcgccgtgt tccccctcgt cttctccggc gccgacctca
      481 tcgcgtgcgc cgagacgggc accggcaaga cggcggcgtt cctcctcccg atcatgcagc
      541 ggctgctcga gcggccggcg gcgggcgcca cgcgcgtgct catcctcgag ccgacgcgcg
      601 aactggcggt gcagatcgag gacgatttca cgggcttctc gtactacacg gacctgaccg
      661 gcgcggccgt ctacggcggc gtcgacggcg acagccaggc gcacgcgctc cgggccggcg
      721 ccgacgtcat cgtggcgacg ccggggcgcc tgctcgatca catgaactgc ggcgtggccg
      781 acttcactag ggtcgaggtg ctcgtcctgg acgaggccga ccggatgctg gacatggggt
      841 tctggcccga cgtgcggcgg atcgtgtccg ccctgccggg cgcgcggcag acgctgctct
      901 tctccgccac gacctcctcc gatgtcgtgg cgctggccga gcaggtgatg cgcgacccgc
      961 gcttcatcca gatcggccgc gccggcgggc ccgcgcgcac catcacccac atcgcgcacg
     1021 tcgtgccggc ccgcgagaag gtcgagtggc tgacgcggtt cctccggcgc tcaatcggcc
     1081 cgtcgctcgt gttcgtgcgc accaagcgcg gcgccgacac gctggcatcg gcgctggcgg
     1141 gccggggcat ccgctgcgcg gcgctgcacg cggaccggac ccagagggac cgatcggccg
     1201 ccgtcgaggg gttccgcgcg ggccggcact ccgcgctcgt cgcgacggac atcgccgcgc
     1261 ggggcctcga catcgacggc gtggcgcacg tcgtcaacta cgacgtgccc gacaccgccg
     1321 agacgtacgt gcaccgcgtg ggccggaccg gccgggcgga agccgccggc accgcgatca
     1381 cgctcgtggc gccggacgaa ctgccggcgc tccaggccat cgagcggtcg atcgacatct
     1441 acatcgaccg ccccgcctga cgcgccccgc cgccggcgga cgcgcggccg ggcagaggga
     1501 ggcaggcgtg cagacgcgac agctcgggac gacggatctt cacctcacgc cgatcggatt
     1561 cggcgcctgg gccatcggcg gcccggacta cgccttcggc tggggccccc aggaggacag
     1621 cgaatcgata gccgcgatcc atcgcgccat cgacgccggc atcaactgga tcgacaccgc
     1681 ggccgtctac ggcctcgggc gatcggagcg ggtcgtggcg aaggcgctgg cgcaggtggg
     1741 cccgtcgcgg cgcccgttcg tgttcacgaa gggctcgctc gtctggaacg agtcgggcgc
     1801 gatcacgcac gccctggccc cggcgtcgct ccggaaggaa gtggacgaca gcctccgccg
     1861 cctcggcacc gacgtcatcg acctctacca gatccactgg cccgagccgt tcggcggcaa
     1921 cggctcggcg ccggagatcg aggaggcctg gcggaccctc ctcgacctgc ggcaggccgg
     1981 gaagatccgg cacctcggcg tctcgaattt cgacgaggtg cagctcgccc gcgtgcacgc
     2041 gatcgcgccc gtggccagcc tgcagccgcc gtactcgatg ctgcgccgcg gcatcgagcg
     2101 ggccgtgctc ccgtactgcc tcgacaaccg gatcggcgtg atcgtgtact cgccgatggc
     2161 ggccggcgcc ctgagcggca cgatgacgcg cgagcgggcc gcctcgctgc cgaagaacga
     2221 ctggcgctcc cagcacgccg acttccggga gccgaagctc agccgcaacc tcgcgctcgt
     2281 cgaggtcctc aggggcatcg gcagccggca cggcgtgtcg ccgggcgtcg tcgcggtcgc
     2341 ctggacgctg cgccacccgg cggtcaccgc cgcaatcgtc gggctgagaa gcccctcgca
     2401 ggtggacggg atcgcgccgg ccggcgagct tcgtctgacg gcggaggaga tagccgagat
     2461 cgagccgcac gtgcaggcgg cgccccggtc cttctagccc gaagggctac cgcccttccg
     2521 cctgggcgtc gagggttccg agccggcggg agacgcgccc gtggagcgcc ggggccgccg
     2581 cgtagcagac cgaggcgagc agggcgccgg cgatcaaatc ggtcacgtag tggtaccgca
     2641 ggtacaccgt ggcgacgagc agcagcgcgc ccgcaaccgt gacgaaccag cggacgcgca
     2701 gccggcggcg ccacgcccac gcgacggtca gcagcgtaac gagggaatgc ccgctcggga
     2761 acgcccccct cggcgcgagg cgcatcgcct cggcgggggt cgcgtcgacc ggcgccatgc
     2821 ctccggcgtc gatcatcgcc cgcaggtgcg gcgtcagcca gaggccgggc agctcgcgcg
     2881 ccgccgatcc gggatcgtgg agcgtgaacc gcgggctgat cgccggcacg agcaggtagc
     2941 cgacgtacgt cacgaagaac ccgaatccgc agacgaagag ccactgcctg aacggccact
     3001 cgcggccccg caggtagaac tcggcgccca ccgccagcgg aatcacgtag taggaccagt
     3061 acgcgacctg gaagatctcc gtcgcaatcg ggtgcgcgaa ccgcgcgatc cacacggtgg
     3121 ggtcgacgcc gaagatccag cggtcggacg cgatgagcca gccgtcgtag agccgccccc
     3181 cgtgcaacgg cccgttcacc aggagcacgg cccggtagat cgcgagcagg tagacggcaa
     3241 acgaccagtc gtgcagcacg cgcacggcgc gcaggtccag gcgcgcgcgc acccaggcca
     3301 gcagcggcac gccgatcacg atcgccgcgc aggtcgccgc cacggtcggc cacgcgggga
     3361 tgtcgccgag gcgcgcgagc gcgatcgcgc cgaggcccga caggaacgcc atcgccagga
     3421 cgtcgatcga cgcgaggcgt cgcgcgatcg tctgggcgcc cggcgccatc atagcgcctt
     3481 cacgtagcag cggcgccggc ggtgctggcg cgactcgagg cccttccact gcgccaggat
     3541 ctgcgtgttg gactcgagca tcgggcccgt ctccgccagc ttgatccggc gcgccaccgc
     3601 ggacttccac gcctcgtgga tgagcaccgc gttcaggccc ttcttctggt agggggggcg
     3661 cacggccgtc agcagcatct cgaggcggtc gctcgtgcgc agatcgcgga gaatccgcag
     3721 gaacccgaac gggaacaggc gcccccggca ctgctggagc gcgcgggcca gcgacggcat
     3781 gacggcgccg aaggcggcga tgttgtcgtc gcggtccagg atgatcgtca cgaaccggtg
     3841 gtccagcacg ccgaagaact ccttcgtgta gtactggacc tgcgcgtccg acagcctgac
     3901 cacgccgtag aggccccggt aggtctcgtt gaccgcctcg aagatgcgct ccacgtacgg
     3961 gaggatgtcc ttcatgctgc gcgtctgcag cacgcgcacc ttcgtgcgct ccagcgccgc
     4021 cttggcgatg cgctcgaggg cctcgggcat gacgggcgga acggggagct gcagctcgac
     4081 ccagtcggcg tccttgcggt agccgagccg ctcgaggtgg cttgcgtaat acgggtagtt
     4141 gtagttggtg atcagcaggt cgagctcctc gaacccctcg acgagcaggc cctggcgatc
     4201 caggtcgcaa aagcccagcg ggccgtgcac ctcagccagc ccgcgcgacc gcgcccagtc
     4261 ctccaccgcg cccaggagcg ccgccgacac ctcggcatcg tcggtgaagt cgagccagcc
     4321 gaaccgcgcg cggcgcttct gccacgtctc gttgtagcga tggctgacga tgcctgccac
     4381 gcgccccgcc gcctcgccgt cgcggaacgc catccagtac ttcgcctcgc agtactcgaa
     4441 ggccggattg cgatcgggcc gcagcgcctt cttctcctcg ccgatgagct tgggcacgta
     4501 gtacgggtgg ccgcggtaga gccgctccgg gaaggcgatg aaccgccgca gatccaaccg
     4561 ggtccggacg tccttgattt cgaccatggg gctccccagc gctcgcggca ggcccgtcgc
     4621 ggagccggcg ggcagccggt tcccctcgag cgccgccttc acggggcgca ggcgcgcgca
     4681 gcgccagccc gtcagcgaac gctcaagtat accgatcggg ccgtcaactc gcgatggtcc
     4741 aggcccgccg cccggtccag ccgcgccagc agatcgggca gagcggccgg aaagcgccac
     4801 tggtcctggc ggccgccggc cgcctccgcc gcgctcgccc acgccggcca ccagggcgcg
     4861 accgcggcgg ccagcggctc gacgaccagg acgcgtgcgc ctcgcgcgtg cgcaccgagc
     4921 agcgcgcgga ggcatcgcac ccgggcctcc tcgccaagct cgttcagcgt gaaggcggcg
     4981 acaatggcgt cggcgcgcgg aggcgcgccg gcgtgcgcca ggtccgcgca gcggacgtgc
     5041 ccgtcgaggc ccaacgcccg cagcgaccgc cgggcttctc cagccgccca cggatggcgc
     5101 tcgatgcccg ccacgcgcgc gcggggcgcg cactcgagcg cccaggccgc cccggccgcg
     5161 cccgtgccgc agccgaggtc gcccacgacg cgggcggcag gccgcgccgc gcccagcgcg
     5221 cgcacgatgg cgcgaaccag caggaagtgc agcggcccgt agaagagcgc aaatgccgcg
     5281 cgcttgccgg cgccgtcgag cgccgcgccc gacgccagcc gcccccgccg ctcgacgtac
     5341 gcggacgaca aagcgcgcac ggcccggctg acctccggaa accgcaggtc gccgaggtgg
     5401 cgcgcctcga gtccgtccat ccagcgctcg aacgccccgt ggccggacga ttcgcgctca
     5461 tcggccatcg gcgtagtcta ccttcactcc cttgcccccg gctgcgcgct gccgggcgtc
     5521 cagcctccgg ccgcgcgccc gttcgtgaat ggagcgcatg ccgggccccg ccctgccgac
     5581 tccgcatgcc ggctcccgca cgtcctccct cctcgtgccc tgtccccgaa tgctatgctt
     5641 gccccgtgcg cctccctgac ggccttctca gcgcgagcgc ggccggcttg gcgaggacgg
     5701 cttccggcat tggcgtcgcg gcggcgctgc gcgcggctcg tctcgtcgag gctcgtcgtc
     5761 cccggcggcg ggcaggccgc ggccgacgct ccgatcgatg acatcctccg ggacgaaaac
     5821 ctcctcttgc ggaatgggtt ggcgtaggag gagtactcgg ggttcggggc tcgggactcg
     5881 ggatttcggg gctcaggact cggggcagag catggatcgt cctctcgtga cgttgaacga
     5941 tgtccgggat gccgcggagc gcatcgcgac catcgcgcgc ctgacgccgg tcgtgccggc
     6001 tgccgaggac tccggcggcc cggacttgct gctcaagtgc gagaacctcc agcggggcgg
     6061 cgccttcaag atccgcggcg cggcgaacat gatcctgcag ctgcccgacg ccgagtgccg
     6121 ccgcggcgtg atcacttact cgtccggcaa ccatggcatc gccgtctcgc tcgcggcgca
     6181 gctcgcggga gtcccggcta tcgtcgtgat gccgacggcg gcgccggccg tgaagacggc
     6241 ggcggtgcgc aggctgggcg cggagatcgt cttcgagggc acgacgacgg tcgagcgcaa
     6301 ggccagagcg gaggtggagg ccgcggcccg gggcctcacg atcgtgccgc cgttcgatca
     6361 ccgctggatc atcgccggcc agggcacgat cggcctcgag ctcttcgccc aggcgccgga
     6421 agccgccgcg gtctacgtgc cagtgagcgg cgggggcctg gtatccggga tcgccgccgc
     6481 cctgaaggcg ctctcccctg ccgtgcgagt gatcggcgtc gagcccgcgg ccgtgccgcg
     6541 catagccagg tcgctcgcgg ccggcggccc gatcaccgtg cctgcgtcct ccggcctcgc
     6601 cgacggcctg ctggcggtgc gtcccgggga cgcgacgttc gcgcacgtgc gggcttacgt
     6661 cgacgagatc gtgacggtga gcgacgaggc catcgcgcgc gccgtgtcgt gggtcttcga
     6721 gcgcgcccac ctcgtggccg agcccagcgg cgccgcctcc gtcgcggccg cgctcggctg
     6781 ggccgagccc gcctggtcga ggcccggcgc cgggcccgct ctgccgtcgc ccggcccgaa
     6841 ggtggccgtc gtgagcggcg gcaacgtcga gaccgatacc ttcatccgct gcatcaggca
     6901 gtgaggcggc gccgcgcgca gatcgggagt ccctataagc tcgggtttgt ggaacctcga
     6961 ctcgatgtca catcctcgga gccctctggc cctgggggaa gatcgacgta gcgctcggcc
     7021 taacgattgc ggcagtccat agtggctagg gcgtctccaa ccgtacgctc gccgagctga
     7081 ccgcctcgct cgaaaaggcg caagcagcca atcggctcca tccgcagcta aagagcctta
     7141 cgcaacccgt cctcctggcc gtcgacgagg ccggctccct ctcgatcagc cgcaccggtg
     7201 ccatgcactt cttccagttg acggccaggc gccacgggca ggcgtcgata acgctcccct
     7261 cgaacaaggg tttcgagcat ttagggcgac aacacatcga cctctggcag gcgctcaatg
     7321 aggcccaggc tgccgacccc gccgcccgac gacatcgacg tacccgtcag ggcttcgcga
     7381 cgagctgagg ctcgtccgtc ggactgtcag actcgcggcc ggcggtagtg tcggactcgc
     7441 ggctggcgtt gacctcaaaa cccgcgaggc acgacaggct gtcccggaag aggcggctgc
     7501 gtccccgcgc gcggggggaa acctagaact gcccgccctt gggcgacccg gacgggtggt
     7561 ggcacgggtt ggcctctttc aacccggacg agttgggcag caggatgttc gcacatcagc
     7621 cccgtttcgg gatcagcatg gcgacggtct cctccccctc tcggggaagc tccggcgctt
     7681 ctggcaagtc ctccgcccac gcgacatccg agtcaccctc ggccagaatg gcggcaagct
     7741 cagccgacgc tgcctcaaac gccttctggt caacgcgagg cgagaaccgc gtcttaaccg
     7801 ttcgcccctt cacgttccat cacctccaca tcagcatgcc caacgcaggg cgtatggcgc
     7861 atgcccagcc gttccagttc ccgggagacc ctggtcgtcg cagattcatg gtcagccgcc
     7921 ttgcctcccc actgtggcac gcacacggag cgcagcacgg tcttgccgct ggcgtgagcg
     7981 ccatcaccgg ggtgcatccc cgcgcacgcg ggggaacccg tgccaaccga aacggctcca
     8041 gcgagaaaac gtctcgggtt ctccgctgcc cagccgcggt agcgaagccg gtcgacatcc
     8101 ggccacgact gatcgaaaga aggcaagacg cctctgcccc ccctccacat cgtcgacccg
     8161 acagtagcct ggaaccgcca gcacaaccgg aggcgggtca cggaaggcct gctggatcgc
     8221 cctccgcacg cgttcatgca gttgagtggc ggctgtacgc gtgggcagcg cgaagtacat
     8281 accgtccacc agcccggcct cgaacaactg aacgaagcga ctcaacgcgg cctcggtctt
     8341 cccgctgcct gtctcggatt ccacgacggt gatcgtgccc gacatggcaa ttggaagctg
     8401 gacaaccgca ctctggatag gccgtggttg gccggcccac tcaggagcta cgcgcgcgta
     8461 tgcgtcgcgc tgttgcgggt ccgttcgatc gcaggcagga tagtggagcc ctatcccgcg
     8521 cagggcgcgt tgggcataac agcgcgaccg ctccatgcga tcggccgtcg cgtcgtcaga
     8581 gaaagggaag tacgcggagt ccgagccaat ccaatccgcc aacatgacga gacccgcgaa
     8641 cgcatgggac agttcggccc ggtccggcag gtctcgtgta cggcgagcga aggccggcgg
     8701 gaaccaagcg cggcaccggg ccaggaggtc agcgacgcct gcctcgggat ccaggtcggc
     8761 cctgggcagc caaacctccg gacgccagca ctgggcggcc tgctcgaagg tgatcgggcg
     8821 accgtggtga ccgagtgcgg caagaagaag ccccgacact ccgtctcccc agtcgcccag
     8881 agcagcgagc cacgtgccaa gcacgccatt cagaacagca atcgcctcgg agacgtggcc
     8941 cgcgggcacg ccgagaccat cccgtccatt cgcctggaaa ccgatgttga acttgcccaa
     9001 gtcgtggagg gctgccagga cggcgagcct ggccctctgg ccgaaatcga gtggctctcc
     9061 cgcgagagca ttgaaccggg cttgccacac cggcagggtc aggagagcct cggcgaccgc
     9121 cgccacgtca gcgcaatggt ccactaaggg gtgccactcg agtacctcct cggcgcgtga
     9181 aggcttcgtc agttttcccc aaaacgtcgt cggcgtacct cgcacgatgc ctcccggccg
     9241 ggctacaccg ccgtctctcc gaggccatca tacgcgtgcg gtgtgacaag ggaggtcgca
     9301 ccgcccccag tctccccatg agtgcgatga acgccccggg gaagatggag tggtggtgcc
     9361 gctcgagcgg atcgcgcagg gcgtcgattc ggtcctgcgc ctcctggagc tgtgggtggg
     9421 gacagcgggc gcgaggcgcg tccgcacggg cctacttctt cgcccgttcc cctttcagct
     9481 ccatcttcat ggcccgccgg atggcctcgg ccatgcggtt cagcttgaca tcggtccgga
     9541 acccaatcga ctgcgtcgat ccgacgatga cggagtacgc gatcttgctg gggacgtcga
     9601 ggtgcttcgc cacgtcgagg atgatgctgc tttcagggtc catcagcgct ccgtcggcca
     9661 gcgcgatgtc catgagccgc gtgaggatga acaccttgtc gtcgagcgag ccgtccttcc
     9721 ggaccgtgtc gagggcgtcg ggcagcctgg ccgcgatggc ctcccggctc gtctcgatct
     9781 cggcctcggg ctcgtcggtg aaccacttgt ggaggatctg gacgaggatc ttcacctcct
     9841 cgtcgctcac gtcgcggtcg gccgcgagca gctgcgcccc cccgagggcg accgcgcgca
     9901 tgacggcgat gtggagcggc tgcgccgggt agcgccgcgt cagcctgacg agatcgtcca
     9961 ccgcgtcctc gagcgcgccg tcggcgatcg gctcgccctc gaccgggtag cccgcgcggg
    10021 ccgccttggc ggagcgcgag aacagctcga ccgccttcag gcggatcggc aggaggggat
    10081 gcgacgcgaa gacctcctcc atcagctcgg gcttgccgcg gatctcgtcc acctggttca
    10141 ccagggcttc gatgtccagg ttcaggttgc gctcgctcag gccgaaggtg gccttcagca
    10201 gcgacgtagc cgtcgccctg aagtcgcggc tcgcgaggac gctgacgcgg tcggcgctga
    10261 tctcggcctt cttccgccac cgcaggtaca ggctctcgcc gagggcgggc agcacggtca
    10321 ccgacgggtt cgacgcgtcg gtcgagatga gcgccgccag ccggttgttc ccgaacagga
    10381 tgtgcccgag ctcgtgcccg aggatagagc gcagctccgc atcgtcgagc cgctcgagcg
    10441 ccggcgccgt gacgcccacg agcgagtagg tcgtgtcctt cctgacgtcg aggatcgcga
    10501 aggcgttgat ctccgtcgag ggcaggcaga agacctcggc ctcggtttcc aggccgaggg
    10561 cggcggtcac gtcgttgaac acccggtaga tgcgcggcga gaggcgctcg ctcagccgga
    10621 tgccctcctt cagcaggcgc tcccggacgc cccggtcgat ctgcctccgg ttctcctcga
    10681 acgtcgccag gtagtcggcg acgcggtagg tcgtcagcaa ctcctcgacg tccttcgcgt
    10741 ccccccggta tcggatgtcg ctccagtcaa tcgccatggt gcccccgccc gagcgatggc
    10801 gcgcgccgcc gcgcgggccg tcattggatc gccaggctgt ggagatccct gaagagcttc
    10861 tcgaagcggt cgagggcggc cccgagctcg ggcagggtcg accgcagggt ggacgtgcgc
    10921 tcgaacaggt acgacgcggg agcgaagctg tcgaagcggg gggcccacgg gggcatcgac
    10981 gcgaagtagg cttccacctc cgccaccacg cgtcgggccg ccgcgctctc gccctcgtcg
    11041 aggacggccg attcctcgag gccgtacgcg ccggtcacga gctcccggaa gagccgccgt
    11101 ccgaggaggt cctccacctg ggcgtcttcg aggtccacgt agaacgccgg ccggaggatg
    11161 cgtccgaact ggagcaggcc gcgctcccag agcccgtcga gccggcgcgg gtggctcatg
    11221 aggccgtcgg tgaggaccgc gattctctgc gcctgcccgg cgaacaggga gacgaacggc
    11281 gcgatgctgt ccagcccgcc gatgggcacg aggctccatg cgggcgaaag gtgcgcccgc
    11341 ccctgcagct gcagctcgcg ggaaaaccac ttcaggtaga gcacgtcgct cgcgcggtcc
    11401 acgagcagcg tctgtggccc cgtcacgacg cgcctggcca cgtcgtaggc gagcgcggcc
    11461 ctgatcggga acagcgtgtc gggctcggtg ctgagcgccc ggtcccccac cttggtgccg
    11521 acgacgctgt cgccgtcgga cacgtcctcc acggtgcgca ggccgtcgag gtagtcgccg
    11581 tcgatcatga agggcgagtg cgtcgtgtag atcacctggt gcgccggcag cagctgctcg
    11641 cggatgaagc gcagcatgtc catctgggcc cgcgcgtgca gcccgagccc gggctcgtcg
    11701 agcaggatga cgtagtccgc cccgtggtcc cgccgcaggt gcgacagcca cacgaggaag
    11761 gagaagaacc agatgaagcc cgtgctgcgc tcgtcgaacc ccagcgtgac ccggtgccgg
    11821 cggttccgga tgcgcatgcg caggatgtac cccgtgtcga agggcgccgg gtcgtgcggg
    11881 cgggcggcgt cgcaccggaa gtccacttcg agcgcgcggt cctggctcca gtactccatg
    11941 acgtcgcgcg agatgcgctg cgacaccgac tcgagctcgg ccacgaggta ctcgaactgg
    12001 tcgatgccgg ccagctcctc cggcgtcgta tgagccagct cgaggagggc gaggaagatc
    12061 gtgtccacga cggtgagggc gcctgcggcc ttccggcgca tcagatcgtc gatggacacc
    12121 tcgcctggca gccggtggta ctcggcgaag tagaggaagc gcggcaggcg cggctccagc
    12181 gcggcggcaa tcgcggacgc gggcgaggcg tccgggaacc gggcctccac atcgaccagg
    12241 agcgcggcga gcgcgggcgt cggcgcctcg aggccgcgaa gcagcgccac cagctccgcc
    12301 acgctcgcgg ccccggagac gcgctcccgc tcgccggcgt cgagccccgc ccggccaagg
    12361 aggcccgcga cgacggcgcg ctcgtccacg tcgatgtgcc accggaggcg gttgtcgtac
    12421 ccgcgcgaca cggacaccag cggctcgggc agggcgcccg ggcccgcggc ctcggcgacg
    12481 gccgccgcgt caggcccctc gaggcgccac gtcgccgtca cgacgcggtc gggcgattgc
    12541 ccgcgccgct tccggtactc ggcccagcgc cggcgcgggt actcggtttc gacgaagtcc
    12601 ccgtcgacgg gcaggatggg attgacgcgg tacagggcct tcaagagcgc cgtcttgccc
    12661 gactcgttct tgccgacgag cgcggtgagc cgccggatgc tgaactccgt cgagtcgtcg
    12721 atgcacttga agttctccac gcggacggac tcaagcagca tgctggacgg gcccccctgg
    12781 ccaacggcaa gatgtagtct gagcggcagc aattgtcaag gaaggcgcgg cgcgccgccc
    12841 gggccgcccg ccccgccgcg tgcagccgcc gcccggcgga gcggtataat cagcggggaa
    12901 tagccctgga gcggcaagag atggacctgc agctcgtgcc ggtggcgatg cccgagaacg
    12961 ccaacgtcgt catcgggcag agccacttca tcaagacggt cgaggatctc tacgagatca
    13021 tggccaccac cgtgccgggc gccgccttcg gcctggcgtt caacgaggcc tccggcgcgt
    13081 gcctcgtccg cagcgacggc aacgacgagg agctgcgcgc cgccgcggtc gacgccgccc
    13141 tggccctggg cgccggccat acgttcgtgc tctacatgcg cggcgcgtac cccatcaacg
    13201 tcctgaaccg gatcaaggac tgccaggagg tgtgccgggt cttctgcgcc acggccaacc
    13261 cgctccaggc cgtggtcgcg gagtcggatc aggggcgcgg catcgtcggc gtcatcgacg
    13321 ggtcgtcgcc caagggcgtc gaagcggagg ccgatcgcga gtggcgcgcc gggttcctgc
    13381 gccggatcgg atacaagcgc tgagcccgcg ccgccccggg tccgccgccg gggctggccg
    13441 gctcgtcccg ctcggtgtac actcgccgat cggcgcctgc gggccgggcc gagcagggcg
    13501 ttggggcgct ccgcctggcg ccaggggccg ggacccgcgc cgcgcgtgag gaggcggaga
    13561 agcacatgag caggcttgcg tggcgcgcgg cgggtctcgc gctggcggcg atggagctcg
    13621 cctcgtgcgg ggcgatcagg tccggggcgg tcaagtcggt cgccaccatg ctctccgaga
    13681 ccgggacgac ggtcagttcg cacgacgacc cggagctcgt ggccgacgcc ctg
//
LOCUS       DAOSIF010000024        13626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4612_length_13626_cov_4.131973, whole genome shotgun sequence.
ACCESSION   DAOSIF010000024 DAOSIF010000000
VERSION     DAOSIF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4612_length_13626_cov_4.131973"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLT35_01780"
     CDS             complement(<1..694)
                     /locus_tag="PLT35_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG27983.1"
                     /translation="MTARKPRAKPRTAFPAAASGPAVQPAPPHPTSARHHPLLIPALL
                     LIVAASGAWAFATSFDGVLVLDDVRAIVRNQTIRTLWPVTIPLSPPSASTVAGRPVAN
                     LTFALSYALGSSTPEAIDPAAFHAANLAIHLTAALLLFGVVRRTLSSPRLRPVFGAAP
                     PWLAGAVALIWVVHPLQTSAVTYVAQRVESLMGLFYLLTMYASIRAADGGRRGRWWTA
                     AAVAGCAAGMATK"
     gene            888..2987
                     /locus_tag="PLT35_01785"
     CDS             888..2987
                     /locus_tag="PLT35_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG27984.1"
                     /translation="MHRLRASVAAVLVGASFAAAGSAPQQAAQPAFRPPQSQPAAAQP
                     QEPVFRSGVDLVPVDVIVVDKDGRPIVGLAPEDFSVTVEGRPRRVVSAEFLSHADRFA
                     TAPDRTQVPTLNVEDRVMMRAEYSSNTRSAPGRLFVLAVDAGNMTRGGGRGAMEAARQ
                     FVQTLAPNDLVALVTLPAGVTVDFTADRAAIREALLKVTGGGANKYLANVNVSLTEAL
                     ALQVGGDRKRIFENAVRMECGFARSDTELQNCRMNLEAEAQSKLFTARASAQSSSRAL
                     EFLIRRLALIEGQKHVIFIGESLVTGASFGLLEGSGDLAWLGSLSQAARVNLYVLHLD
                     RAFLEAFDVLERFPSRTPLEDARLLNDGLGELAGQAGGAYFNLTVAIDPAFERIARET
                     SASYLVAFEPAPDDRDGKAHNVSIKVARPGVHVRARRQFTVDPAAASASMAQRVTRAL
                     NSPHLPVTVPMDLTTYVIGDSRAGGVRVLMAAEIGCGAGTTADFEVGHLVTDSSGRGA
                     GSAVQRPAGLIERGGGVRCVYYSSGFGIKSGEYVVRAVAIDSANRAGGVERRLDARLA
                     PAGPLGLSSLVLTDPAVRVDDKMKLVVDGRVAGPSVAAYVEMRRLQPAVDAPAAALRP
                     AVQFEVARAEDGPALVGTVPPVADEADGGAWYAEGAVSLATLAPGSYVVRALVTWDGR
                     IVGRASRSIILERAASQ"
     gene            complement(3084..6077)
                     /locus_tag="PLT35_01790"
     CDS             complement(3084..6077)
                     /locus_tag="PLT35_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG27985.1"
                     /translation="MRNLALLFLMLALAVPAMAQEQRASIEGVIKDASGAVMPGVTVE
                     VRNLEVGSVVSTVTDATGVYRFPALPPGTYDVTASLQGFNTIRSANVPLALGQIKKVD
                     LALTVAGVTESVQVTAESPLVDVKQSARATSIRGEQIDLLPKGRDFSSLVTQAPGANN
                     EAKLGGLSIDGASAGENRYIVDGVEATNIISGTQGKELLTDFIEEVQVKSSGYTAEFG
                     GATGGVVSAVTKSGTNNFRGSLRVYFEGDVLASARSKSLRLNPTNSEVAEYWTYPEDE
                     WTRFDPGFSLGGPIWRDKAWYFVAYQPRISKWTRSVSPETAGTPTATPVSGVTQKYLG
                     RNFSANQTAQISNSLRTRIAYNNSWLKDTGNLPALAGTDAPGSAYDIDWTYPNWSASA
                     QIDWVATPNVFIGVRGGYYFSDAFSEGIPTDIRYIFSTGNNIGLTGLDGTVVPASYGR
                     PTGFQNVTTNWSTTRDQQTRLNGQIDATLYLNLAGQHTWKAGVQFDRIGNNVLDGEQA
                     NIVRIRWNSNLGGVRGNYGYYQVRSNGVNPRMGFLTEGDVYLNNYGFFLQDAWTVNNK
                     LTVNLGIRTEQEKVPAYTTGEGIPVYGIEFGFKDKLAPRVGFAYDVKGDGKWKAYGSW
                     GVFYDIFKLALPRGLFGGDKWLEYYYTLDTYAWDTLASSSGCPPACSGTLFRGPVDFR
                     HATFGSDAIEPNLKPMKMQEASLGLEHQLSPTMAISARYVRKWLNRAIEDTGSLDAAG
                     NEIYIIANPGEGLTKLAWPDPAVDQPKAKRDYDSVELAFTKNLSNRWYFRGSYMWSRL
                     YGNYSGLSQSDENGRTDPNSGRSFDYPLMSFDGKAKPVFGVLATDRTHQVKAQFIYQM
                     PWGTSVGLNEYIASGLPVTREVAVIPGSNFPMMYLGRGSDGRMPMYSRTDLSVQHAFK
                     FAGSRQLHLELNVDNVLNQRVAVAKFSTLNRPGEAVGFSETAFYLGQVDVSNLVGTLK
                     PDARFLKDLDFQGGRSARVGVRFLF"
     gene            complement(6364..7050)
                     /locus_tag="PLT35_01795"
     CDS             complement(6364..7050)
                     /locus_tag="PLT35_01795"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOG27986.1"
                     /translation="MKTNWRLELALLFLIAAMFAAAILVWPNAPSDIPVHWNASGEVD
                     RYGGKVEGLLGLPLMSLAIYLLMRFLPRIDPGRANYARFSGAYAAMRVGIQALLALIH
                     AVVLAWISGRRIDVSQIVPLGVGALFVLLGAVLGKVRPNWFVGIRTPWTISSKRSWVR
                     THRLGGWLFMALGILFALTGAFRLGRFGPGILAAVVGVVLVLSVYSYLVWRADPEKQA
                     PAGTQPADEE"
     gene            complement(7047..7337)
                     /locus_tag="PLT35_01800"
     CDS             complement(7047..7337)
                     /locus_tag="PLT35_01800"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG27987.1"
                     /translation="MRINDIFQALSDPTRREILRLLRERDMSAGELADCFPLARSTLS
                     SHFKSLRQAGLIVPEKRGTTIRYSLNVSAAEEALGLIMDALRVGKARKGTPS"
     gene            complement(7501..8844)
                     /locus_tag="PLT35_01805"
     CDS             complement(7501..8844)
                     /locus_tag="PLT35_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOG27988.1"
                     /translation="MRRQAAALALAVTAGAVLAAGLSAARLGYPPEEFIGRRQALAKA
                     VGSGTVVLFGRTEAQAGIRFRQDNDFYYFTGNEDLNAAMVLEAPSGSATLYLPRQTAS
                     EIRAHGKNWLTDAGLGKPWGFAAIRPLDDLPLAVARVAGSADSRLWLRLAAGDEADVN
                     RSEHATSLARRLVNLGGTALSEDASRAAYIRSHFPALQVADVTPFIDRMRTIKSAREI
                     AVLRENGRISAEAIRRAILATRRNGFEYEIEAEATYWLLRNGVQGNAFPAIVGSGPNV
                     NVWHYFTNGRQYEDGDLIVMDYGGALDYLAVDITRTWPVSGTFTPDQLKAYECALAAQ
                     KAIIAAMTVGATRAETRAIGEAVYKQHGVPGGASAGHYVGMSTHDVGDASLPFRAGMV
                     LAVEPIVEDASKQIHVRIEDTVLITEQGPEILTSGVPKEIDDVLALVRQARAAGR"
     gene            complement(8903..9784)
                     /locus_tag="PLT35_01810"
     CDS             complement(8903..9784)
                     /locus_tag="PLT35_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG27989.1"
                     /translation="MTSPVRGLTGFSRAMFSNWLWHEPLGLVIDAGEGLQLALGQRIW
                     SPRTVALTHGHADHVLGLPGFIASRRYAKGAQDKPLAIVYPEGNAGVLAVRELVGRLW
                     PNEAFPVTWTGVRAGDEFRLGKALGLRAFAATHGGAEPTLGYVVFEERRRLKPAFAGL
                     AESEVRQLAREGGREALMEPYRHVRLAHSGDSMPVDPSVAAGADLLVHDATFLDAADR
                     RREIHATASEALEVGRAAGVRCLVLQHLSIRYDRASAIPALREQVAASGFAGACWLLD
                     DGRLTALNTGPGLWPEA"
     gene            9929..11008
                     /locus_tag="PLT35_01815"
     CDS             9929..11008
                     /locus_tag="PLT35_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOG27990.1"
                     /translation="MRILVDSSAFMDQFERDLAQATRSVAIQAMSFEGDQAGLRLARL
                     LRDRHDLDRTLVINRYSLFYVSDCFLPAPWNAVSASLWRERALTLAAVRELRRHGVTV
                     FWTNPVGLAFRKFIARNHKKSVVIDRRAVYFGGMNFCDHNFDWHDVMLRVEDEELGAF
                     MCEEIQATLRGGNQSIRRDFGGIEILLLDGVGNEEGFEPVFRLICEARRSIVVESAYV
                     TFPFYSHLAQAVRRGIAVTIIAPEHNNKPNMNRYTQWEAARAGIELHLYPGRMNHIKA
                     MLVDDEALIMGSSNFDYLSVHAYQEVVAIVREPAVIEQYRREVLDADLARCVAPTRRF
                     SAAWQSARRAAFGTLGRAAVGLSRL"
     gene            complement(11028..>13626)
                     /locus_tag="PLT35_01820"
     CDS             complement(11028..>13626)
                     /locus_tag="PLT35_01820"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOG27991.1"
                     /translation="ERLLDVEIVVSPYCHVMGAYGVALLTQRVVQEQGRAATAFRGLD
                     LDEREIRLSKQTCDLCNNHCGITLATIDGEAGPSWGYMCGREPGERRMRVTPHSRLLR
                     RRRRDWLEGGRGIEVPDGAPVIGIPQALSIYTYLPLWRRFFNALGYRVLLSGGTTQAI
                     RELGGRMSGAEFCFPAKSFLGHVASLAAKEGVDFVFLPEMKNEVPNEHVTTSTFCPYV
                     QASPSYARAALALNAMDTVRLLAPIIDLRLSERTLLKRLASELGGPLGRSPREIAAAW
                     RDGYAAQRAFEQRLRDEGARAIEQARAAGEKLLVLIGRPYNLYDSGLNLDLPDKLAEF
                     GRTVLPIDFLTLDLSRLGERYRNTYWGYGQKILAALEDVASNGLLDAVYFTNFSCGPD
                     SFLLSYASEIMGNKPFLALELDEHGSDTGYMTRIEAFFDVLRRPRKDAARRRAPVAPA
                     SQAIDGRTLWVPDMHPYSTEFVAAAFRGQGYRARALPLETEETFELGRSLTRGSECLP
                     TALTIGSLLTAMRTSPPDEKHAYFLATAQGPCRFGQYVTLHRQILDREGFPDVPILAP
                     TSFMGYSIINEAMRKTLFKVLVTGDLLMKAGCRVRPYERETGETNYRLERARRRIVDV
                     LEGGGDVARELAAAVRDIASVPRKDVPPKPLVGIVGEIYVRNNAFANEHVVQSIEKLG
                     AEAWMVPLIEWILFVTSPYNVRTHLDHKLSKAMVSTFLTYKWMQRWERKLYRAASPLL
                     DDRHEPPLGDVLEAAMTIMPDSIGGEALLTVGRTIAFARQQAAMVVNVAPFSCMPGTI
                     TTALFRKVSADTGMPIVNMFYDGTGGQNRSLEVYLRTALNADSDHLEDGASLAGRHGH
                     MLPVYPVTD"
BASE COUNT         2037 a         4707 c         4733 g         2149 t
ORIGIN      
        1 ccttcgtggc catcccggcc gcgcagcctg cgacggccgc cgccgtccac cagcgcccgc
       61 ggcgtccgcc gtccgccgcc cgaatcgagg cgtacatggt cagcagatag aaaagcccca
      121 tcaacgactc gacgcgttgc gcgacgtagg tcaccgccga cgtctgcagc gggtgcacga
      181 cccagatgag ggcgacggcc cccgcgagcc atggcggcgc ggctccgaag accgggcgga
      241 gtctcggaga cgacagcgtc ctccgcacga cgccgaacag cagcaaggcg gccgtcagat
      301 ggatcgcgag gttggcggcg tggaacgcgg ccggatcgat cgcctccggc gtggacgatc
      361 cgagcgcata gctgagggcg aaggtgaggt tggccacagg ccggcctgcc accgtggagg
      421 cggacggcgg cgacagggga atcgtcaccg gccacaacgt tcggatcgtc tggttccgca
      481 cgatcgcgcg cacgtcgtcc aggacgagga cgccgtcgaa actggtcgcg aaggcccacg
      541 ccccggacgc ggcgacgatc aggagcagcg cggggatcaa cagcggatga tggcgagccg
      601 acgtcgggtg cgggggcgcg ggctgcaccg caggaccgga cgcggcagca gggaaggcgg
      661 tgcgcggctt cgccctgggc ttcctggcag tcacacccgg gatcttactc cggccgttcg
      721 cctgtcccga aggcgcggct tcagcggggc cagcggcgtc ccgagttccg gctccgcacg
      781 cgctccatgg ccctctctgg cgactggccc tagatgcccg ctccgagcgc cgccgtcttc
      841 ggcggttccc gggggtacta tgaagccaca gcttcggaga ctccctcatg catcgtcttc
      901 gtgcttcagt cgctgccgtc ctggtcgggg cgtcgttcgc cgcggctggt tccgcgcccc
      961 agcaggcggc gcagccggca ttccggccgc cgcagtcgca gccggcggcc gcgcagccgc
     1021 aggagcctgt tttccgctcc ggcgtcgatc tcgttcccgt cgatgtgatc gtcgtggaca
     1081 aggacggccg cccgatcgtg ggccttgccc ccgaggactt cagcgtcacg gtcgaaggca
     1141 ggccgcgccg cgtcgtgtcg gccgagttcc tgtcgcatgc cgaccggttc gcgacggcgc
     1201 ccgaccgcac gcaggtcccg accctcaacg tcgaagaccg ggtcatgatg cgcgcggagt
     1261 acagctcgaa cacgaggtcc gccccggggc gcctgttcgt gctcgccgtc gacgcgggca
     1321 acatgacgcg cggcggcgga cgcggggcga tggaagcagc ccggcagttc gtccagacgc
     1381 tggcgccgaa cgacctggtg gcgctcgtga cgctcccggc gggcgtgacc gtcgatttca
     1441 ccgccgaccg cgccgccatc cgggaggccc tgctcaaggt caccggcggc ggggccaaca
     1501 agtacctcgc gaacgtgaac gtcagcctga ccgaggcgct cgcgctgcaa gtgggcggcg
     1561 atcggaagcg gatcttcgag aacgccgtgc ggatggagtg cggcttcgcg cgctccgaca
     1621 cggagctgca gaactgccgg atgaacctcg aagccgaggc ccagagcaag ctgttcacgg
     1681 cgcgggcgtc ggcgcagtcg agcagccggg cgctcgagtt cctcatccgc cgccttgcgc
     1741 tcatcgaggg ccagaagcac gtcatcttca tcggcgaatc gctcgtcacg ggagcgtcat
     1801 tcggcctgct cgaggggtcc ggcgacctgg cgtggctcgg gagcctgagc caggcggcgc
     1861 gcgtgaacct ctacgtgctc catctcgacc gggcgtttct cgaagcgttc gacgtgctcg
     1921 agcgctttcc gtcgcggacg cccctggaag acgcccggct gctcaacgac gggctggggg
     1981 agctggccgg gcaggccgga ggcgcgtact tcaacctgac ggtcgcgatc gacccggcct
     2041 tcgaacggat cgcgagggag acctcggcgt cgtacttggt ggcgttcgaa ccggcaccgg
     2101 acgaccgcga cggcaaggcc cacaacgtct ccatcaaggt ggcgcggccg ggcgtgcacg
     2161 tcagggcgcg ccggcagttc acggtggatc cggccgccgc gtcggcctcg atggcgcagc
     2221 gtgtcacgcg ggcgctcaac tcgccgcacc tgccggtcac cgtgccgatg gacctcacga
     2281 cgtacgtcat cggcgactcc cgagccggcg gcgtgcgcgt gctgatggcc gccgagatcg
     2341 gctgcggcgc cggcacgacg gccgacttcg aggtgggcca cctcgtgacc gattcgtccg
     2401 ggaggggcgc cggctccgcc gtccagcggc cggccggact catcgagcgc ggaggcggcg
     2461 tgcgctgcgt ctattactcg tccggctttg gcatcaagag cggggagtac gtcgtccggg
     2521 cggtggccat cgactcggcg aaccgggccg ggggcgtcga gcgccgtctc gacgcgcgcc
     2581 tcgcgccggc ggggccgctc gggctgagca gcctggtcct gaccgaccct gccgtgcgcg
     2641 tcgatgacaa gatgaagctc gtggtggacg gccgcgttgc gggcccgagc gtggcggcct
     2701 acgtcgagat gcggcggctg cagccggccg tggacgcgcc agcggccgcg cttcgcccgg
     2761 ccgtccagtt cgaggtggcc cgggccgagg acgggccggc actggtcgga accgtccctc
     2821 cggtggctga cgaggcggat ggcggcgcgt ggtatgccga aggcgccgtc agcctggcga
     2881 cgctggctcc cggctcgtac gtggtgcgcg cgctggtgac gtgggacggc aggatcgtgg
     2941 gccgcgcgag tcgatccatc atcctggaac gcgccgcgag ccagtaggcc gcaaaaggaa
     3001 cggccccgca ggccgcgatg gcgtgcgggg ccggggtcca tcggatcgcc ggggcccagg
     3061 ccccggcgcc ggttcgaatc agctcagaac aggaaccgga cgcccacgcg ggccgaccgg
     3121 ccgccctgga agtcgagatc cttcaggaag cgcgcgtcgg gcttcagggt gccgaccagg
     3181 ttcgagacgt cgacctggcc gaggtagaac gcggtctcac tgaagccgac cgcctcgccc
     3241 gggcggttca gggtactgaa cttggccacc gcgacgcgct ggttgagcac gttgtccacg
     3301 ttcagttcca ggtgcagctg cctgctgccc gcgaacttga acgcgtgctg aacgctcagg
     3361 tccgtccgcg agtacatggg catgcggccg tcgctgccgc ggccgaggta catcatcggg
     3421 aagttgctcc ccgggatcac cgccacctcg cgggtcaccg ggagtccgct cgcgatgtat
     3481 tcattgagcc ccacggacgt gccccagggc atctgataga tgaactgcgc cttgacctgg
     3541 tgcgtgcggt cggtggcgag gacgccgaag accggcttgg ccttcccgtc gaagctcatg
     3601 agcggatagt cgaacgatcg cccggagttc gggtctgtgc ggccgttctc gtcggattgc
     3661 gacaggccgg agtagttgcc gtagaggcgg ctccacatgt agctgccgcg gaagtaccag
     3721 cggttcgaca ggttcttcgt gaaggccagc tccacgctgt cgtagtcgcg cttggccttc
     3781 ggctgatcca cggccggatc gggccacgcg agcttcgtca ggccttcgcc cgggttggcg
     3841 atgatgtaga tctcgttgcc ggcggcgtcg agcgagcccg tgtcctcgat ggcgcggttg
     3901 agccacttgc ggacgtaacg ggcgctgatg gccatggtcg gcgagagctg gtgctcgagg
     3961 cccagcgagg cctcctgcat cttcatgggc ttgaggttgg gctcgatcgc gtccgagccg
     4021 aacgtcgcgt gacggaagtc caccgggccg cggaagagcg tgcccgagca ggccggcggg
     4081 cagccggacg aggacgccag ggtgtcccac gcgtaggtgt cgagcgtgta gtagtactcg
     4141 agccacttgt cgccgccgaa caagccgcgc ggcagcgcca gcttgaagat gtcgtagaac
     4201 acgccccagg acccgtacgc cttccacttg ccgtcgccct tgacgtcgta ggcgaagccg
     4261 acgcgcgggg ccagcttgtc cttgaagccg aactcgatcc catagaccgg gatgccctcg
     4321 cccgtggtgt aggccggcac cttctcctgc tcagtgcgga tgccgaggtt gacggtcagc
     4381 ttgttgttga ccgtccaggc atcctgcagg aagaacccgt agttgttgag gtagacgtcg
     4441 ccctcggtca ggaatcccat cctcgggttg acgccgttgc tgcggacctg gtagtagccg
     4501 tagttgccgc gaacgccgcc gaggttcgag ttccagcgga tgcggacgat gttcgcctgc
     4561 tcgccgtcga ggacgttgtt gccgatccga tcgaactgca cgccggcctt ccacgtgtgc
     4621 tggccggcca ggttgaggta cagcgtcgcg tcgatctggc cgttcaggcg cgtctgctgg
     4681 tcacgcgtgg tggaccagtt ggtggtgacg ttctggaaac ccgtcgggcg tccgtacgac
     4741 gccggcacca ccgtgccgtc gaggccggtc aggccgatgt tgttgcccgt gctgaagatg
     4801 tagcggatgt cggtcggaat cccttcgctg aacgcgtcag agaagtagta cccgccgcgc
     4861 acgccgatga acacgttcgg cgtcgccacc cagtcgatct gcgccgacgc gctccagttc
     4921 gggtacgtcc agtcgatgtc gtacgccgag cccggcgcgt ccgtgccagc gagggccggc
     4981 aggttgccgg tgtccttgag ccagctgttg ttgtaggcga tgcgcgtgcg caggctgttg
     5041 ctgatctgcg cggtctggtt cgcggagaag ttgcggccca ggtacttctg ggtcacgccg
     5101 gacaccggcg tggcggtggg cgtgccggca gtctccggcg acacgctgcg cgtccacttg
     5161 ctgatgcgcg gctggtaggc gacgaaatac cacgccttgt cccgccagat cgggccgccc
     5221 agcgagaagc cgggatcgaa ccgcgtccac tcgtcctccg ggtacgtcca gtactctgcg
     5281 acctcggagt tcgtcgggtt cagccgcagc gacttgctcc gcgcgctggc cagcacgtcg
     5341 ccctcgaagt agacgcgcag gctgccgcgg aaattgttcg tgccgctctt cgtgacggcg
     5401 ctgaccacgc cgcccgtggc gccgccgaac tcggcggtgt agcccgacga cttgacctgc
     5461 acctcttcga tgaagtcggt cagcagctcc ttgccctgcg tgccgctgat gatgttggtc
     5521 gcctcgacgc cgtccacgat gtaacggttc tcgccggcgc tcgcgccgtc aatcgagagg
     5581 ccgccaagct tggcctcgtt gttggcgccc ggcgcctgcg tgaccagcga cgagaagtcg
     5641 cggcccttcg gcagcaggtc gatctgctcg ccgcggatgc tggtggcgcg cgcgctctgc
     5701 ttcacgtcga cgagcggcga ctcggcggtc acctgcacgc tctccgtgac gcccgcgacg
     5761 gtcagcgcca agtccacctt cttgatctgg ccgagggcaa gcggcacgtt ggccgacctg
     5821 atcgtattga agccctggag cgacgccgtg acgtcatacg tgcccggcgg gagcgccgga
     5881 aaacggtaga cgcccgtggc gtcggtcacg gtcgagacga ccgagccgac ctccaggttt
     5941 ctcacctcaa cagtgacgcc cggcatcacg gcgccggacg catccttgat gacgccctcg
     6001 atagacgccc tctgctcctg cgccatcgcc ggcaccgcca gcgcgagcat caggaacagc
     6061 agggcaaggt tcctcacacg cattctcctc atcctccttg caggtgaacc gactcgctct
     6121 cgagtcctga ccagccgggc gggttagccg agacccgcac gcggtggtac ttgctatggt
     6181 attaatgcac gcatcgtgcc aagcgaggac attgctaaat gattgataaa tagattcttg
     6241 gtgtttggga gttactcggc atgaacctgt catccactgc tttggagagt agcgatccaa
     6301 ctgcttggac gtttgccgca gggtcgcgca cgcgcgccga ctgtctcgac gcctccgcga
     6361 gcctcattcc tcgtcggcgg gctgagttcc ggccggggct tgcttctccg ggtccgcgcg
     6421 ccagacgagg tacgagtaga ccgacaggac gagcacgacg ccgaccactg cagccagtat
     6481 gcccgggccg aaccggccga gcctgaaggc gccggtgagg gcgaagagga tgccgagggc
     6541 catgaagagc cagccgccca ggcggtgcgt gcgaacccag gagcgcttgc tcgagatcgt
     6601 ccacggcgtg cggatgccga cgaaccagtt cggccgcacc ttgccgagca cggcgccgag
     6661 caagacgaac agcgcgccga cgccgagcgg cacgatctga gacacgtcga tccgccggcc
     6721 cgagatccac gcgaggacga cggcgtggat gagcgcgaga agcgcctgga tgccgacgcg
     6781 catcgcggcg taggcgccgg aaaaccgggc gtagttggcg cggccgggat cgatgcgcgg
     6841 caggaaccgc atcaacaggt agatcgccag ggacatcaac ggcaggccga ggagcccctc
     6901 gaccttgccg ccgtaacgat cgacctcgcc ggaggcgttc caatggacgg gaatgtcgga
     6961 cggggcgttc ggccacacga ggatcgccgc tgcgaacatg gctgcgatca ggaagaggag
     7021 ggcgagttcg agacgccagt tggtcttcac gacggggttc ccttccgggc cttgccgacg
     7081 cgcagcgcat ccatgatcaa gccgagcgcc tcctcggcgg ccgagacgtt gaggctgtag
     7141 cggatggtgg tgccgcgctt ctccggcacg atgaggccgg cctgcctgag cgacttgaaa
     7201 tggctggaga gggtggacct ggcgaggggg aagcagtcgg ccagttcacc ggcgctcatg
     7261 tcgcgctcgc gaagcaggcg gaggatctcg cgccgcgtcg ggtcgctgag ggcctggaag
     7321 atgtcgttga tcctcatcgg cgcgctcgct tggccccgtg gctcgcgaga ggccggtatc
     7381 ctggactccc gctaagtttc gatatttcgc caacaataga aatatagccc gaaacccgct
     7441 cgccgtcaac gcacctggag gcgtcgacgc cccgaatccc gggttccgag tcccgagccc
     7501 ctagcgtccg gccgcccgcg cctgcctgac aagcgccagc acgtcgtcga tttccttggg
     7561 cacgcccgac gtcaggatct cgggtccctg ttcggtgatg agcaccgtgt cctcgatccg
     7621 gacgtggatc tgcttcgagg catcctcgac gatgggctcg actgcgagga ccatgccggc
     7681 ccggaagggc aggctggcgt ctcccacgtc gtgcgtgctc atcccgacgt agtggccggc
     7741 gcttgcgccg cccggcacgc cgtgctgctt gtagacggcc tcgccgatgg cgcgcgtctc
     7801 ggcgcgcgtc gccccgaccg tcatggcggc aatgatggcc ttctgcgccg caagcgcgca
     7861 ctcgtaggcc ttcagctggt ccggcgtgaa cgtccccgac accggccagg tgcgcgtgat
     7921 gtcaacggcg aggtaatcga gggcgccgcc gtaatccatc acgatgaggt ccccgtcctc
     7981 gtactgccgg ccgttggtga agtagtgcca gacgttcacg ttcgggcccg agcccacaat
     8041 ggccgggaac gcgttgcctt ggacgccgtt ccgcagcagc cagtaggtcg cctcggcctc
     8101 gatctcgtac tcgaagccgt tgcggcgcgt ggcgagaatg gcccgccgga tggcctctgc
     8161 gctgatgcgt ccgttctcgc gcaggaccgc gatctcgcgg gcgctcttga tcgtccgcat
     8221 gcggtcgatg aacggcgtga cgtctgcaac ctggagggcg gggaagtgcg agcggatgta
     8281 ggcagctcgg ctcgcgtcct cgctcagcgc cgtgccgccc aggttgacga ggcgtcgggc
     8341 caggctggtg gcatgctcgc tgcggttgac gtcggcttcg tcgccggccg ccaggcgcag
     8401 ccagagccgc gagtccgccg acccggccac gcgggccacg gcgagcggca ggtcgtcgag
     8461 cgggcggatc gcggcgaagc cccagggctt gcccaggccg gcatcggtga gccagttctt
     8521 gccgtgcgcc cgaatctcgc tcgcagtctg gcgggggagg tacagcgtgg ccgaaccgga
     8581 cggcgcctcg agcaccatcg ccgcgttcag gtcctcgttg ccggtgaaat agtagaagtc
     8641 gttgtcctgg cggaaccgga tgccagcctg ggcctccgtc cggccgaaca gcacgaccgt
     8701 gcccgatcca accgccttgg ccagcgcctg gcgccggccg atgaactcct cgggcggata
     8761 cccgagccga gccgcgctca gtccagccgc cagaaccgct cctgccgtca ccgccagcgc
     8821 caacgctgct gcctgccgcc gcattcccag cctcccttcg cgaagggcca gtcccctcga
     8881 ccccccgagg gccctggcgt tctcaggcct ccggccagag gcccggccct gtgttcaacg
     8941 cagtcagcct gccgtcgtcg agcagccagc acgcgccggc gaagccggac gcggccacct
     9001 gttcccgcag cgccggaatc gccgaggccc ggtcgtagcg aatcgacagg tgctgcagca
     9061 cgaggcaccg cacgccggcc gcgcggccca cctcgagggc ctccgacgcc gtggcgtgaa
     9121 tctcccgcct gcgatcggcg gcgtcgagga acgtggcgtc gtgcacgagc aggtcggccc
     9181 cggcggccac cgacgggtcg acgggcatgc tgtcgccgga atgcgccagg cgcacgtggc
     9241 ggtagggctc catcagcgcc tcgcgcccgc cttcgcgcgc cagctgccgc acctcggact
     9301 cggcgagccc ggcgaaggcc ggcttgaggc ggcgccgctc ctcgaacacc acgtagccca
     9361 gcgtcggctc ggccccgccg tgcgtcgcgg cgaacgccct gagtcccagc gccttgccga
     9421 ggcggaactc gtcgcccgcc cggacgcccg tccacgtcac ggggaacgct tcattcggcc
     9481 agaggcggcc gacgagctcc cgcacggcga ggacgccggc gttgccctcg gggtacacga
     9541 tggccagcgg cttgtcctgc gcgcccttgg cgtaccggcg cgaggcgatg aaccccggca
     9601 gtcccagcac gtggtccgcg tgaccgtggg tcagcgccac ggttctcggc gaccagatcc
     9661 tctggccgag cgccagctga agcccctcgc cggcgtcgat gacgaggccc aggggctcgt
     9721 gccacagcca gttgctgaac atggcgcggg agaagccggt gagaccgcgg acgggagacg
     9781 tcacgcggat atgttacacg gtggccgcgt tccgtcggcc ccgctcgccc cggcggccga
     9841 gccagccgcc acgggccttg ggcgcccggc gtcgccgcgt gtacactgcc tcacgccggc
     9901 cgcccgattg gcggccgctc gtgcgcccat gaggatcctc gtcgacagtt cggcattcat
     9961 ggatcagttc gagcgggacc tcgcgcaggc gacgcgctcc gtcgcgatcc aggccatgtc
    10021 gttcgaggga gaccaggccg gcctgcgcct cgcccggctg ctccgcgatc gccatgacct
    10081 cgatcgcacc ctggtgatca atcggtactc gctcttctac gtgagtgact gcttcctgcc
    10141 agccccgtgg aacgccgtct cggcctcgct gtggcgggag cgcgcgttga cgctcgcggc
    10201 ggtccgtgag ctgcgccgtc acggggtcac ggtcttctgg acgaatccgg tcggcctggc
    10261 gttccggaag ttcatagccc ggaaccacaa gaagtcggtc gtcatcgacc gccgcgccgt
    10321 ctacttcggc ggcatgaact tctgcgatca caacttcgac tggcacgacg tgatgctgag
    10381 ggtcgaggac gaggagctgg gcgcgttcat gtgcgaggag atccaggcga ccttgcgcgg
    10441 cgggaaccag tcgatccgcc gcgacttcgg cggaatcgag atcctcctgc ttgacggcgt
    10501 cgggaacgaa gaggggttcg agcccgtctt ccggctgatc tgcgaagccc gccggtcgat
    10561 cgtcgtcgag agcgcctacg tcaccttccc gttctactcg cacctcgcgc aggcggtccg
    10621 ccgcggcatc gcggtgacaa tcatcgcgcc cgagcacaac aacaagccga acatgaaccg
    10681 gtacacgcag tgggaagcgg cgcgcgcggg catcgagttg cacctgtacc ccggccgcat
    10741 gaaccacatc aaggcgatgc tcgtcgacga cgaggccctc atcatgggat cgtcgaactt
    10801 cgactacctc agcgtgcacg cgtaccagga agtcgtggcg atcgtgagag agccggcggt
    10861 tatcgagcag taccgccgcg aggtgctgga cgccgatctc gcccggtgcg tcgcgccgac
    10921 gcggcgtttc tcggcggcct ggcagtccgc acggcgggcc gcgttcggca cgctgggccg
    10981 ggcggctgtc ggcctcagcc ggctctgacg ctgccgccgg cccgctctca gtccgtcacc
    11041 gggtagacgg gcagcatgtg gccgtgccgc ccggcgaggc tggccccgtc ctcgagatgg
    11101 tcgctgtcgg cgttcagtgc cgtcctgagg tacacctcga ggctccggtt ctgcccgccg
    11161 gtcccgtcgt agaacatgtt gacgatgggc atgccggtgt cggcgctgac cttccggaac
    11221 aaggccgtcg tgatcgtgcc gggcatgcag ctgaacggcg cgacgttcac gaccatggcg
    11281 gcctgctgcc gcgcgaaggc gatcgtccgg cccaccgtca gcagcgcctc gcccccgatg
    11341 ctgtccggca tgatcgtcat ggcggcctcg agcacgtcgc cgagcggggg ctcgtggcgg
    11401 tcgtcgagca gcgggctcgc cgcgcggtac agcttccgct cccagcgctg catccacttg
    11461 tacgtcagga acgtcgacac catcgccttc gacagcttgt gatcgaggtg cgtccgcacg
    11521 ttgtagggcg acgtgacgaa caggatccac tcgatgaggg gcaccatcca cgcctcggcg
    11581 cccagtttct cgatcgactg cacgacgtgc tcgttcgcga aggcgttgtt gcggacgtag
    11641 atctcgccga cgatgccgac cagcggcttc ggcgggacgt ccttccgcgg caccgacgcg
    11701 atgtcgcgca cggccgccgc cagctccctg gcgacgtcgc cgccgccctc gaggacgtcg
    11761 acgattcgcc tgcgagcccg ctcgaggcgg tagttggtct cacccgtctc gcgctcgtac
    11821 ggacggaccc tgcagcccgc cttcatcagg agatcgccgg tgacgaggac cttgaacagc
    11881 gtcttccgca tggcctcgtt gatgatcgag tagcccatga agctcgtcgg cgccaggatg
    11941 ggcacgtccg ggaagccctc gcggtcgagg atctggcggt gcagcgtgac gtactggccg
    12001 aaccggcagg ggccctgcgc cgtggcgagg aagtacgcgt gcttctcgtc cggcggcgac
    12061 gtccgcatgg ccgtcagcag gctgccgatc gtcagcgccg tcggcaggca ttccgacccg
    12121 cgcgtcagcg atcggcccag ctcgaacgtc tcctccgtct cgagcggcag cgcccgcgcc
    12181 cggtagccct ggccgcggaa ggccgccgcg acgaactcgg tcgagtacgg gtgcatgtcc
    12241 ggcacccaca gcgtccgccc gtcgatcgcc tgcgacgcgg gcgcgaccgg cgcccgccgc
    12301 cgcgccgcgt ccttgcgcgg ccgccgcagc acgtcgaaga acgcctcgat gcgcgtcatg
    12361 tagcccgtgt ccgagccgtg ctcgtccagc tcgagcgcga ggaacggctt gttgcccatg
    12421 atctcggacg catagctcag caggaagctg tcgggcccgc agctgaagtt cgtgaagtag
    12481 accgcgtcga gcaggccgtt ggatgcgacg tcctccagtg cggcgaggat cttctgcccg
    12541 tagccccagt acgtgttccg gtagcgctcg ccgagccgcg agagatcgag cgtcaggaag
    12601 tcgatcggca ggaccgtccg gccgaactcc gcgagcttgt cgggcagatc gaggttcagg
    12661 ccgctgtcgt agaggttgta cggccgcccg atcagaacca ggagcttctc gcccgcggcg
    12721 cgcgcctgct cgatcgccct ggcgccctcg tcccgcaggc gctgctcgaa cgcccgctgc
    12781 gcggcgtatc cgtcgcgcca cgccgcggcg atctccctgg gcgaccggcc gagcggcccg
    12841 cccagttcgg aggccagccg cttgagcagc gtccgctcgc tcagccggag atcgatgatc
    12901 ggcgcgagca gccggacggt gtccatggcg ttcagggcga gcgccgcccg cgcgtacgac
    12961 ggcgacgcct gcacgtacgg gcagaaggtg gacgtcgtca catgctcgtt gggcacctcg
    13021 ttcttcatct cgggcagaaa gacgaagtcc acgccctcct tcgcggcgag cgacgccacg
    13081 tggccgagga agctcttcgc ggggaagcag aactccgcgc cgctcatccg gccgcccagc
    13141 tccctgatcg cctgcgtggt gccgcccgac agcagcacgc ggtagccgag cgcgttgaag
    13201 aagcgccgcc acaacggcag gtacgtatag atgctcaggg cctgcgggat gccgatgacc
    13261 ggcgcgccgt cgggaacctc gatgccgcgc cccccttcga gccagtcgcg cctgcgccgc
    13321 ctcagcaggc gactgtgcgg cgtgacgcgc atcctgcgct cgcccggctc gcggccgcac
    13381 atgtagcccc acgacggccc cgcctcgccg tcgatcgtgg cgagcgtgat cccgcagtgg
    13441 ttgttgcaga ggtcgcacgt ctgcttcgac agccggatct cgcgctcgtc gaggtccagc
    13501 ccccggaagg ccgtcgcggc ccgcccctgc tcctgcacga cccgctgcgt cagcagcgcc
    13561 acgccgtagg cccccatgac gtggcagtag ggcgacacca cgatttccac gtcgagcagc
    13621 cgctcg
//
LOCUS       DAOSIF010000025        13278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4743_length_13278_cov_3.690161, whole genome shotgun sequence.
ACCESSION   DAOSIF010000025 DAOSIF010000000
VERSION     DAOSIF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13278
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4743_length_13278_cov_3.690161"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLT35_01825"
     CDS             complement(<1..927)
                     /locus_tag="PLT35_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG27992.1"
                     /translation="MRTPAVLIGVMLAASLAQAQAPAPPVVGAWEGALDAMGAKLRIG
                     VAITAGPDGALQATLDSPDQGAFKLPLSDVAFADGVLTFTLAAARGRFEGRLNEAGTE
                     IAGTWTQAGPLPLVLRRVEALSGPRRPQEPKPPFPYRTEDVRVPNPAGGSVLAGTLTL
                     PEGPGPFPGAVLVTGSGAQNRDEEIMGHKPFLVLADYLTRQGIAVLRYDDRGVGESTG
                     DLAAATSEDFAGDAEAVWRALRARPDIDGARVGFIGHSEGAIIAPMIAARTPGVAFIV
                     MIAGSGVPGDEIMVRQSTSILQASGVPAEAVAA"
     gene            complement(1150..1584)
                     /locus_tag="PLT35_01830"
     CDS             complement(1150..1584)
                     /locus_tag="PLT35_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOG27993.1"
                     /translation="MREAPQPGPAKETAGAVRLDVWLDVACLFKTRSEAQKACRGGKV
                     EVNGQGAKPHRTIVVGDRLRITRGPGRKQHVVVQSLAAQHVPKAEARRLYEDVTPPPT
                     PEELAVRELERHFWKARVPRLPRTAPDRRERRRLLREKEGAD"
     gene            complement(1581..2198)
                     /gene="rdgB"
                     /locus_tag="PLT35_01835"
     CDS             complement(1581..2198)
                     /gene="rdgB"
                     /locus_tag="PLT35_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOG27994.1"
                     /translation="MAGPRLVVATTNRHKLREIRAVLDGLGTVIDGLDAFPPVAEPEE
                     TGTTFAENARQKAVYYSSALGVPVVAEDSGLEIDGLGGEPGVRSARYGGPDRPAYEQK
                     FALVYAGLRDRGASGSPARFVCALTLADGARVVFEALGTIEGRIADEPAGAGGFGYDP
                     IFFYPPLGRTLAQLDEREKAGVSHRGRAFRQLRAFLASGGPLRGE"
     gene            complement(2198..2917)
                     /gene="rph"
                     /locus_tag="PLT35_01840"
     CDS             complement(2198..2917)
                     /gene="rph"
                     /locus_tag="PLT35_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOG27995.1"
                     /translation="MTRSDNRGPADLRPVTMTPRFLVHPEGSVLIEAGRTKVICTASV
                     EDRVPPFLRNSGKGWVTAEYGMLPRSTNTRSTREASAGRVGGRTHEIQRLIGRSLRAV
                     TRMEELGERTIWIDCDVVQADGGTRTASITGSYVALALALKTLVTRGALPRVPLSGQV
                     AAVSVGIVGGVPLLDLAYDEDSRADVDMNVVKTSDGRYIEIQGTAEAAPFGRDQLAGL
                     LDLADQGLARLIALQQEVLQS"
     gene            complement(2914..3525)
                     /locus_tag="PLT35_01845"
     CDS             complement(2914..3525)
                     /locus_tag="PLT35_01845"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOG27996.1"
                     /translation="MASRARSIALTLIAAAAVGTGTWATLRTLARQRPAPPTRADSRA
                     AGPMDPGAAPAAPAARRIKATLYYVSADGLGLEAAERDVPFADGASEQGKRIVEALLE
                     PAPDGRVSAIPPGTTLRALYLGGRGDAYADFSDGLRAHHPGGSQHELLTVYAIVDTLT
                     ANLPAITSVQILVDGREVDSLAGHVDLRRPLPNTRLRAEKGTP"
     gene            complement(3530..5086)
                     /locus_tag="PLT35_01850"
     CDS             complement(3530..5086)
                     /locus_tag="PLT35_01850"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG27997.1"
                     /translation="MRVRAPLTALLLLAAAAPGAAQSLRPAAPYALVAADGRRAIQAA
                     VAGGQDLVRLDEIAAGLQLTVQEDRASKALTVSRGLNVVVLSLDQPLASTGGRVMSLP
                     VAPIRDGQRWWVPPDIVGRAFGAVAGVRVEVRKASRVILVGDVRVPRLAIRQEQAGSA
                     SRLSIDISPRAAYSIAQEPRRLLVRFDADGLDAGPIQAGGGLVDGVAIVEPSTVAIAL
                     AGGYGSYRSATLPLDAASSRLVIDVLPAGVAGAAAPPAPAPARPDARPQTANPFEPAP
                     AGPPPALPPPPGIRTIALDPGHGGDEHGAVGQGGLREKDVVLDVARRLKGQIEGRLGI
                     RVLLTRDDDRLVPHDERASIANNNKADLFVSLHANSSPIRSARGAQVYSLSPDGLSAE
                     ARRQADSPDARPVPVLGGGSRDIDLILWDMAQVRHMAESSALAVLIEEELRRRVEMNA
                     HPVQQAPFRVLVAANMPAVLVEMAFLSNPEDERLLATDEFKMKIVQALYDAIVRYRAR
                     AEAQGALRRP"
     gene            complement(5217..7577)
                     /locus_tag="PLT35_01855"
     CDS             complement(5217..7577)
                     /locus_tag="PLT35_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOG27998.1"
                     /translation="MTARTRRLREESLAAVPSISGERAAILTEFYKAHLGRHSAPVLR
                     AMSFYALCDRKTLWMGDGELIVGERGPRPKAVPTFPELTCHSAEDLRVLDSRPKTRYA
                     VDPSVIELYTREIIPFWAGRSLRDQMFRELPPEWKDAYDAGCFTEFMEQRAPGHTVAD
                     GKIYRKGLLDFKAEIAAAEAALDFAGDPEALDKRDELRAMSIACDAVMRFAERHAEMA
                     EAAAAKEPDERRRRELRKIAGVCRHVPARAPCDFHEALQQYWFCHLAVVTELNGWDSF
                     SPGHLDQHLLPFYQAGLEAGTLTRDSARELLECFFVKFNNHPAPPKVGVTAAESGTYT
                     DFATINLAGLLRDGRDGSNEVTRLLLDIIDEMHLLQPSSNVQVSRKTPDAVLKHALRV
                     IRKGYGFPSLFNADAVVEEQLRQGKSLEDAREGGCSGCVEVGAFGKEAYILTGYFNLA
                     KMLELALHDGFDVRTGKRVGARTGDPAGFASFDDLFAAFEAQVRHVVDVKIRGNQLVE
                     RLYATRMPHTFLSVITDDCIAKGRDYNAGGARYNNTFIQAVGIGTVTDSLSALKELVY
                     ERGDLPLARFVEILDSDFTGHEELRQRLLHKTHKYGNDDDYADALMTRTFGMLFEAID
                     GRPNTKGGAYRLEMLPTTCHVYFGEVCLASADGRGEGHPVSEGISPVQGADRRGPTAV
                     MKSASKMDHIKTGGTLLNMKFAPGLVEGDAGVDGLHGLVRAYFKLDGHHVQFNVVSAD
                     LLREAKANPAAHRGLIVRVAGYSDYFCDLSEALQDEIIERTEHGGF"
     gene            complement(7574..8449)
                     /locus_tag="PLT35_01860"
     CDS             complement(7574..8449)
                     /locus_tag="PLT35_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOG27999.1"
                     /translation="MGSGLVFSVQRFSLHDGPGVRTTVFMKGCPLGCAWCHNPESRSP
                     EPEYVRLRRLCMSCSRCTDVELDSPVVTGRGAEDVAACPTGALRGVGERTDARALVRD
                     VLRDRAFFEESGGGVTFSGGEPLAQAAFVTEALALLQAEGVHTALDTCGFGPWPALRS
                     AAERANLVLYDLKLIDDVRHRSATGASNALILANLRSLAQVHRALWIRVPVVPGVNDD
                     DANMEATAAFVRSLPGVGRVDLLPYHQAGEAKFARAGLETPLRGTPPPPPGRLESLAR
                     AFWARGLHTTIGGRA"
     gene            complement(8449..9186)
                     /locus_tag="PLT35_01865"
     CDS             complement(8449..9186)
                     /locus_tag="PLT35_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG28000.1"
                     /translation="MSVSDALHRATAPEPPPLGEAPRLNTRSLREQVYDYLRAEMARG
                     GLQPGGFLDLTDIARRLGISRTPLREALLHLEAQGFVSVFPRRGFRLNDLTLDDIRHF
                     YEIIGALEASALRSVGRSLGPADFSRMRQLDADMADAVAARDFDRYYAANLAFHDVYL
                     ARSDNARLVAQVRLLKQRLYDWPRRRSMLQAWEQHSMVEHEDFLRLLERGDIDEAAAH
                     LQCVHWSFAVQEPFIHAYYFAAGPGAP"
     gene            complement(9255..9923)
                     /locus_tag="PLT35_01870"
     CDS             complement(9255..9923)
                     /locus_tag="PLT35_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28001.1"
                     /translation="MNRCCPALLLSLFLVMPAAPALAQNAESKKVTLDLNAVAPQAAF
                     NAVAEAIGVTASVDPSLTSPVDIAVRNVSARTALTAICESIGCTWELTSARLVVRAGS
                     DAGPIAARVSVGGKAATPEAQAVVDAFRKELPAGLNFEHAPLADVNKRLSEAVGLPVQ
                     LKGRVPDVTTLTMDFSGLTLQSALQKIGEREERPKAAWQLIVGPAPGAKAPQIAIAVG
                     PAKQ"
     gene            complement(9920..10699)
                     /locus_tag="PLT35_01875"
     CDS             complement(9920..10699)
                     /locus_tag="PLT35_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG28002.1"
                     /translation="MASQGSLAIVDLEGTVVALAPRVLRYATARLGDRALAEDVAQES
                     LAALVRACRNGGAPASPDAFVFAIARRRAARASWRQRLWLPLESAAGNRSHAPTPEAR
                     ALARDEAERVRAAIARLSSRDREAILLVAAAGLSMADAASALRISVPAVKRRVSRARA
                     RLAARVAARTLASAPGSRHSRMALAAAAAVLVLAAALTVRQSAELPAPPAVPDAISGT
                     LSDGLLVARMPDGSTSITGGPARQSRSPEGHGLLLVEGEAR"
     gene            10797..11144
                     /locus_tag="PLT35_01880"
     CDS             10797..11144
                     /locus_tag="PLT35_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28003.1"
                     /translation="MRWVFAIIIALHGLIHFMGPAKAFGLADLPQLTRPLSRGMGLAW
                     LLAGLLTLAAVAALFAWPRCWWVIGAAAAVLSQAVIFTSWTDARAGTLANLILLAGVA
                     CGVLTGWPGRSPS"
     gene            11319..12245
                     /locus_tag="PLT35_01885"
     CDS             11319..12245
                     /locus_tag="PLT35_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOG28004.1"
                     /translation="MSARKPHRAPTPAMTRLDGLIAAPFTPFDRKGDVSLPVIAQQVR
                     HLLKTGVRGAYVCGTTGEGISCTVAERKAILEEWVRRSGGRLVIIAHVGALSVRDAQE
                     LAAHAQACGADAASIVPPNFFKPASVAGLVAHVAAVAAAAPRLPFYYYHTMMSGVDLS
                     MPAFLEAAERSIPTLAGVKFNSFNLYEYQNCLRACGGRYDIVYGVDEVFAGALTLGAR
                     AFIGSTYNYAAPLYHAIREAVDAGRLEEARAGMARVCAVVDLLEQHGGLAAGKAMMGA
                     HGIDVGDPRLPLGALAPARKAALVRRLREILA"
     gene            12254..>13278
                     /locus_tag="PLT35_01890"
     CDS             12254..>13278
                     /locus_tag="PLT35_01890"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOG28005.1"
                     /translation="MRTLAAGMHASRLDALVRVYRDGLLQDTVPFWLRHGADPEYGGI
                     MTCLDRDGSLVDTDKGVWQQGRFAWTLGVLVNTVERRPEWLAAAEHTLRFIEARCIDA
                     DGRMFFHVTRDGRPIRKRRYAFSEAFAAMAFGEHARATGRARSRDMAERLYLAYAGHE
                     PSPPKWRDTRPMKSMSGPMIQIAVCQALRESIGLARADGDIDRAIAEIRRDFMKPDLR
                     CCLEHVGPDGAVIGHADGRLLNPGHAIEGAWFLMLEGKTRGIGEYVAAGCDILDWMWE
                     RGWDQVHGGLLSFADLDGKPVAEYWHDMKFWWPHNEAIIATLLAYQLTGDARYARLHE
                     RAHDYAYG"
BASE COUNT         1803 a         4727 c         4849 g         1899 t
ORIGIN      
        1 ggccgccacc gcctcggccg gcacgccgct cgcctggagg atcgaggtgg actggcgcac
       61 catgatctcg tcgccgggca cgcccgagcc ggcgatcatg acgatgaagg cgacgccggg
      121 cgtccgcgcg gcgatcatcg gcgcgatgat ggcgccttca ctgtggccga tgaagccgac
      181 ccgcgcgccg tcgatatcgg ggcgcgcgcg aagcgcccgc cagacggctt ccgcgtcgcc
      241 cgcgaagtcc tcgctcgtcg ccgccgccag gtcccccgtc gactcgccga cgccgcggtc
      301 gtcgtagcgc aagaccgcga tgccctgccg ggtgaggtaa tcggcgagga cgaggaacgg
      361 cttgtggccc atgatctcct cgtcgcggtt ctgcgcgccg ctgccggtca cgagcaccgc
      421 gccggggaac gggcccgggc cctcgggcag cgtgagcgtg cccgcgagca cgctgccgcc
      481 agcggggttg ggcacgcgca cgtcctcggt gcgatagggg aacggcggct tcggctcctg
      541 cggccgtctc ggccccgaga gcgcttcgac ccgcctcagg accagcggca gcggaccagc
      601 ctgcgtccac gtgccggcaa tctcggtgcc ggcctcgttc agcctccctt cgaaccgtcc
      661 ccgcgcggcg gccagcgtga aggtcagcac gccgtcggcg aatgccacgt cgctcagcgg
      721 cagcttgaag gcgccctgat ccgggctgtc cagcgtcgcc tgcagggccc cgtccggccc
      781 tgcggtgatc gcgacgccaa tcctgagctt cgcgcccatg gcatcgagag ccccctccca
      841 tgcgcccacc accggcggcg ccggagcctg cgcttgcgcg agtgacgccg cgagcatgac
      901 gccgatcaga acagctggtg ttcgcatcgt tccaccccgg tgatgtggcc tcttccgttc
      961 gccccgagtt ccagtccccg aatcccgagc cccgagtccc gaaccccgtc tgttccttgc
     1021 cccccgcctt cgctagacgg cctgccggcc agcttcggcg ggcaggcttt gcctgcccct
     1081 tgccccttgt ccctgtctgc ctggcgccca gcacccagcc cctagcgccg agcgcctggc
     1141 ccctagcgcc taatctgccc cctccttctc cctcaggagc cgccgtcgtt cccgccgatc
     1201 gggcgcggtg cgcgggagac gcgggacgcg ggccttccag aagtgacgct cgagttcccg
     1261 cacggccagc tcctcgggcg tcgggggcgg cgttacatcc tcgtacaagc gcctggcctc
     1321 ggccttcggc acgtgctgcg cggcgagcga ttggacgacg acgtgctgct tccgacccgg
     1381 gccccgggtg atgcgcaggc ggtcgccaac gacgatggtg cgatgcggct tggcgccctg
     1441 gccgttcacc tcgaccttcc cgccccggca cgccttctgg gcctcggatc gcgtcttgaa
     1501 gaggcaggcg acgtcgagcc agacatcgag ccggacggcg ccggccgttt ccttcgcggg
     1561 cccgggctgc ggcgcctccc tcattcgccc cggagcgggc cccccgacgc caggaacgcg
     1621 cgcagctgtc ggaaagcgcg gccgcggtgg ctgacgccgg ctttctcgcg ctcgtcgagc
     1681 tgggcgagcg tccggccgag aggcgggtag aagaagatcg ggtcgtatcc gaacccgccg
     1741 gcgcctgcgg gttcatccgc gatccgccct tcgatcgtgc cgagcgcctc gaacaccacg
     1801 cgggcgccgt ctgcgagcgt cagcgcgcag acgaaccgcg cggggctgcc gctggcgcca
     1861 cggtcccgga ggcccgcata cacgagggcg aacttctgct cgtaggccgg tcgatcgggg
     1921 ccgccgtagc gcgccgagcg cacgcccggc tcgccaccga gcccgtcgat ctcgaggcct
     1981 gagtcctcgg ccaccaccgg cacgccaagc gccgacgagt aatagactgc cttctgccgc
     2041 gcgttctcgg cgaaggtcgt gccggtttcc tccggttccg ccaccggcgg aaaggcatcg
     2101 aggccatcga tgacggtgcc gaggccgtcc aggacggcgc ggatctcgcg gagcttgtgg
     2161 cggttggtcg tggcgacgac gagccgcggg ccggccatca cgattggagc acttcctgct
     2221 gcagcgcgat caggcgggcg aggccctggt cggccaggtc gagaaggccg gccagctggt
     2281 cgcgtccgaa cggggctgcc tccgccgtgc cctggatctc gatgtagcgg ccgtcggagg
     2341 tcttgacgac gttcatgtcc acgtccgcgc gggagtcctc gtcgtaggcc aggtccagca
     2401 gcggcacgcc gccgacaatc cccacgctga cggctgccac ctggcccgac agggggacgc
     2461 gcggcagcgc gccccgcgtg accagcgtct tcagggcgag cgccagcgct acgtagctgc
     2521 ccgtgatgga cgccgtccgc gtgccgccgt cggcctggac gacgtcgcag tcgatccaga
     2581 tcgtccgctc gccgagttcc tccatccgcg tcacggcacg cagcgagcgc ccgatgagcc
     2641 gctggatctc gtgggtgcgg ccgccgaccc ggcccgccga cgcttcccgc gtcgaccggg
     2701 tgttcgtcga gcggggaagc atgccgtact cggccgtcac ccagcccttg ccgctgttgc
     2761 gcaggaaggg cggcacccgg tcctcgacgc tcgccgtgca gatcaccttg gttcgtcccg
     2821 cctcgatcag gaccgagccc tcggggtgca cgaggaaccg gggcgtcatc gtcacggggc
     2881 gcaggtccgc gggcccccgg ttgtcgcttc gagtcatggc gtgcccttct ccgcccgcag
     2941 acgggtgttc ggcagggggc gacggagatc gacgtgtccc gccagggaat cgacctcccg
     3001 cccgtcgacg aggatctgca ccgacgtaat cgccggcagg ttggcggtga gcgtgtccac
     3061 gatggcgtac accgtgagca gctcgtgctg cgatccgccg gggtgatgcg cgcggaggcc
     3121 gtcgctgaag tccgcgtacg catcgccgcg cccgccgaga tagagggccc ggagcgtcgt
     3181 gcccggcggg atggccgaca cgcgcccgtc cggcgccggc tcgagcaggg cctccacgat
     3241 ccgtttgcct tgctcggacg cgccgtcggc aaacggcacg tcgcgctcgg cggcctcgag
     3301 ccccaggccg tcggccgaga cgtagtagag ggtcgccttg atgcgccggg ctgccggcgc
     3361 ggcgggagcg gccccagggt ccatcgggcc cgccgcgcgg gagtcggcgc gggtcggcgg
     3421 cgcgggccgc tggcgcgcga gggtgcgaag ggtggcccag gttcccgtgc cgacggcggc
     3481 ggcagcgatc agggtcagcg cgatggaccg tgcgcgggat gccatggcgt cacgggcggc
     3541 ggagcgcgcc ctgcgcctcc gcgcgggcgc ggtagcggac gatcgcgtcg tagagggcct
     3601 ggacgatctt catcttgaac tcgtcggtgg ccagcagccg ctcgtcctcg gggttgctga
     3661 gaaacgccat ctcgacgaga acggccggca tgttggcggc gaccagcacg cggaacggcg
     3721 cctgctgcac cgggtgcgcg ttcatctcga cgcgccgccg caactcctcc tcgatgagca
     3781 ccgccagcgc ggacgactcg gccatgtgcc gcacctgcgc catgtcccag aggatcaagt
     3841 cgatgtcgcg cgacccgccg ccgagcaccg gaacgggccg cgcgtccggc gagtcggcct
     3901 ggcgccgcgc ctcggcgctc aggccgtccg ggctgaggga gtagacctgc gcgcccctgg
     3961 cgctgcggat cggcgacgag ttcgcgtgaa gcgagacgaa caagtcggcc ttgttgttgt
     4021 tcgctatcga cgcccgctcg tcgtggggca cgaggcggtc gtcgtctcgc gtgagcagca
     4081 cgcggatgcc gagccggccc tcgatctggc ccttgagccg gcgcgccacg tcgagcacga
     4141 cgtccttctc gcgcaggccg ccctgcccga ccgcgccgtg ttcgtcgccc ccgtggcccg
     4201 gatcgagggc gatcgtccgg atgcccggcg gcggcggcag cgcgggaggc gggcccgccg
     4261 gcgccggctc gaaggggttc gcggtctggg gtcgcgcatc cggcctcgcg ggcgccggcg
     4321 ccggcggggc ggcagctccg gcgacgccgg cgggcaggac gtcgatgacg agccgcgacg
     4381 acgccgcgtc gagcgggagc gtcgccgagc ggtaggagcc gtagccgccc gccagggcga
     4441 tcgccaccgt cgacggctcg acaatcgcca ccccgtcgac gagcccgccg ccggcctgga
     4501 tcggcccggc gtcgagcccg tccgcgtcga agcgcacgag caggcggcgg ggctcctgcg
     4561 cgatggagta cgccgcgcgc gggctgatgt cgatcgacag gcgcgatgcg ctcccggcct
     4621 gctcctgccg gatcgcgagg cgcggcacgc gcacatcgcc gacgaggatc acgcgcgacg
     4681 ccttccgcac ctcgacgcgg acgccggcga cggcgccgaa cgcgcggccg acgatgtcgg
     4741 gagggaccca ccagcgctgg ccgtcgcgga tgggcgcgac gggcagcgac atgaccctgc
     4801 cgcccgtgga ggccagcggc tggtcgagtg agaggacaac cacgttgagg ccgcgcgaga
     4861 ccgtcagcgc cttcgacgcg cgatcctcct gcaccgtgag ctgcagaccg gcagcgatct
     4921 cgtcgaggcg gacgagatcc tgcccgcccg cgacggcagc ctggattgcg cggcggccgt
     4981 cggcggcgac gagggcgtac ggcgcggccg gacgcagcga ctgggccgcg ccgggcgcgg
     5041 cggcggccag caggaggagg gccgtgagcg gagcgcgcac tctcacaggc tcccatcata
     5101 ccgccgaacc gcgagccgtc cgcacgcctc ccaggcgcgt ggcgcaacag ctcgacagcg
     5161 gggccgcgct tcggtgtgac cgcccgaatc tctggccccg agcccagggt ccctgcctag
     5221 aaccccccgt gctcggtgcg ctcgatgatc tcgtcctgga gcgcctccga gaggtcgcag
     5281 aagtagtcgc tgtaccccgc gacccggacg atgaggccgc ggtgggccgc ggggttggcc
     5341 ttggcctccc ggaggagatc ggccgagacc acgttgaact gcacgtggtg gccgtccagc
     5401 ttgaagtagg cgcggacgag gccgtggaga ccgtcgacgc ccgcgtcgcc ctcgacgagg
     5461 cccggcgcga acttcatgtt gagcagcgtc cccccggtct tgatgtggtc catcttggac
     5521 gccgacttca tcacggcggt cggcccgcgc cgatccgcgc cctgcacggg gctgatgccc
     5581 tccgagaccg ggtgcccttc gccgcggccg tcggccgagg cgaggcacac ctcgccgaag
     5641 tacacgtggc aggtggtcgg cagcatctcg aggcggtacg cgccgccctt cgtgttgggc
     5701 cgcccgtcga tcgcctcgaa gagcatcccg aaggtccgcg tcatcagcgc gtccgcgtag
     5761 tcatcgtcgt tgccgtactt gtgcgtcttg tggagcaggc gctgccgaag ctcctcgtgg
     5821 cccgtgaagt ccgagtcgag gatctcgacg aagcgggcga gcggcaggtc gccccgctcg
     5881 tacaccagct ccttgagcgc tgacaggctg tcggtgacgg tgccaatgcc gacggcctgg
     5941 atgaacgtgt tgttgtagcg ggcgccgccg gcgttgtagt cccggccctt cgcgatgcag
     6001 tcgtcggtga tgacggacag gaacgtgtgc ggcatgcgcg tcgcgtagag ccgctcgacg
     6061 agctggttgc cgcggatctt gacgtcgacg acatggcgca cctgcgcctc gaaggccgcg
     6121 aagagatcgt cgaaagaggc gaaccccgcc gggtcgcccg tccgggcgcc cacgcgcttg
     6181 ccggtgcgca cgtcgaaccc gtcgtgcagc gccagctcga gcatcttcgc caggttgaag
     6241 tagccggtga ggatgtacgc ctccttgccg aaggcgccca cctccacgca gcccgagcat
     6301 ccgccctccc tggcgtcctc gaggctcttg ccctgccgca gctgctcctc gacgaccgcg
     6361 tcggcgttga acagcgacgg gaacccgtag cccttgcgga tgacgcgcag cgcgtgcttc
     6421 agcaccgcgt ccggcgtctt gcgcgacacc tgcacgttgc tgctcggctg caggaggtgc
     6481 atctcgtcga tgatgtcgag cagcaggcgc gtgacctcgt tcgagccgtc gcggccgtcg
     6541 cgaagcagtc ccgcgaggtt gatggtggcg aagtccgtgt acgtgccgct ctcggccgcc
     6601 gtcacgccga ccttcggcgg cgccgggtgg ttgttgaact tgacgaagaa gcattcgagc
     6661 agctccctcg cggagtcccg tgtgagcgtg cccgcctcga ggcccgcctg gtagaagggc
     6721 agcaggtgct ggtcgagatg gccggggctg aacgagtccc agccgttcaa ctcggtcacg
     6781 acggcgagat ggcagaacca gtactgctgc agcgcctcat ggaagtcgca cggcgcgcgc
     6841 gccgggacgt ggcggcacac gccggcgatc ttccgcagtt cgcggcgccg gcgctcgtcc
     6901 ggctccttcg ccgctgccgc ctcggccatc tcggcgtggc gctcggcgaa gcgcatcacc
     6961 gcgtcgcagg caatggacat ggcgcgaagc tcgtcgcgct tgtccagcgc ctcgggatcg
     7021 cccgcgaagt cgagcgcggc ctccgcggcc gcgatctccg ccttgaagtc gaggaggccc
     7081 ttccggtaga tcttgccgtc cgcgacggtg tgccccggcg cgcgctgctc catgaactcg
     7141 gtgaagcagc ccgcgtcgta ggcgtccttc cactcggggg gcagctcgcg gaacatctgg
     7201 tcccgcaggg agcggcccgc ccagaagggg atgatctcgc gcgtgtacag ctcgatgacc
     7261 gacggatcca ccgcgtagcg cgtcttcggg cgagagtcga ggacgcggag gtcctccgcg
     7321 ctgtggcagg tcagctccgg gaaggtgggc acggctttcg gccgggggcc gcgctcgccg
     7381 acgatgagct cgccgtcgcc catccagagg gtcttccggt cgcacagcgc gtagaagctc
     7441 atcgcgcgca gcacgggcgc gctgtggcgg ccgagatgcg ccttgtagaa ctccgtcaga
     7501 atggcggcgc gctcgccgct gatggatggg acggcggcga ggctctcctc gcggagacga
     7561 cgggtgcgtg cggtcatgcg cggcctccga tggtcgtgtg caggccccgg gcccagaagg
     7621 cgcgcgccag cgattcgagt cgcccgggcg ggggcggggg cgtgccgcga agcggcgtct
     7681 ccaggcccgc gcgggcgaac ttcgcttcgc cggcctggtg gtaggggagc aggtcgacgc
     7741 ggccgacgcc gggcagcgag cgcacgaacg ccgccgtcgc ctccatgttg gcgtcgtcgt
     7801 cgttcacgcc cggcacgacg ggcacacgga tccagagcgc gcgatgcacc tgcgcgaggc
     7861 tccgcagatt ggcaaggatg agcgcgttgg aggcaccggt ggcggagcgg tgacggacgt
     7921 cgtcgatgag cttgaggtcg tacagcacca ggttcgcgcg ctccgccgcg gagcgcagcg
     7981 ccggccaggg cccgaacccg cacgtgtcga gcgccgtgtg aacgccctcg gcctggagca
     8041 gcgcgagcgc ctcggtgacg aacgccgcct gcgcgagggg ctcgccgccc gagaaggtga
     8101 cccccccgcc cgattcctcg aagaaggcgc ggtcgcggag gacgtctcgg acgagcgcgc
     8161 gcgcgtccgt ccgctcgccc acgccccgca gcgcgcccgt cgggcacgcg gcgacgtcct
     8221 ctgcgccgcg gcccgtgacc acggggctgt ccagttcgac gtcggtgcag cggctgcagc
     8281 tcatgcacag gcggcgcagc cggacatact ccggctcggg cgaacggctc tccgggttgt
     8341 ggcaccaggc gcagccgagc gggcagcctt tcatgaagac cgtggtgcgc acgcccggcc
     8401 cgtcgtggag cgagaagcgc tggacgctga agaccaggcc gcttcccatc acggcgctcc
     8461 ggggccggcc gcgaagtagt acgcgtggat gaagggctcc tggaccgcga aggaccagtg
     8521 cacgcactgc aggtgcgcgg ccgcttcatc gatgtcgccc cgctcgagga gccgcaggaa
     8581 gtcctcgtgc tcgaccatcg agtgctgctc ccaggcctgg agcatcgatc ggcggcgcgg
     8641 ccagtcgtag aggcgctgct tgaggaggcg gacctgcgcg accaggcgcg cgttgtcgct
     8701 acgcgccagg tagacgtcgt ggaacgcgag gttcgcggca taataccgat cgaagtcgcg
     8761 ggcggcgacg gcgtcggcca tgtcggcgtc gagctgtcgc atgcgggaga agtcggcggg
     8821 gccgagcgag cggccgaccg atcggagggc ggacgcctcg agggcgccga tgatctcgta
     8881 gaagtgccgg atatcgtcga gggtgaggtc gttcaggcgg aagccgcggc gggggaagac
     8941 ggagacgaag ccctgggctt cgaggtgcag cagggcctcg cgcaggggcg tcctgctgat
     9001 gccgaggcgc ctggcgatgt cggtgagatc gaggaacccg ccgggctgga gcccgccgcg
     9061 ggccatctcg gcgcggaggt agtcgtagac ctgctctctg agagatctgg tgttcagccg
     9121 gggagcctct cccagagggg gaggctccgg cgccgtggcg cggtgaagcg cgtccgagac
     9181 gctcatctga aatgtaatat atcagattcg aggcctggct tggaggcaac ttgggtctcg
     9241 gcctcctacc tgccctactg cttggccggt ccgacggcga tggcgatctg cggcgccttc
     9301 gcgccgggcg cggggccgac gatgagctgc cacgcggcct tgggccgctc ttcacgctcg
     9361 ccgatcttct ggagcgcgct ctggagcgtc aggccgctga aatccatcgt cagcgtggtg
     9421 acgtccggga cccggccctt gagctggacg gggaggccaa cggcttcgct cagtcgtttg
     9481 ttgacgtccg cgagcggagc gtgctcgaag ttcaggcccg cgggcagctc cttccggaac
     9541 gcgtccacga cggcctgggc ctcgggcgtc gccgctttcc cgccgacgga gacacgggcc
     9601 gcgatcgggc cggcgtcgct cccggcccga acgacgagcc gcgcgctcgt caactcccag
     9661 gtgcagccga tgctctcgca gatggcagtg agggccgtcc tggcgctgac gttcctgacc
     9721 gcgatgtcca cgggactggt cagcgacgga tctacgctgg ccgtgacgcc gatggcctcc
     9781 gcgacggcgt tgaacgcggc ttgaggcgcg acggcgttga ggtccagcgt gaccttcttg
     9841 gattcggcgt tctgcgccag cgccggagcc gcaggcatca cgaggaacag cgacagcagc
     9901 agggcagggc agcaacggtt catcgggctt ctccttcgac gagcaacagg ccgtggcctt
     9961 caggcgaccg gctctggcgg gccgggcccc cggtgatcga tgtggaccca tcgggcatcc
    10021 gggcgacgag cagcccgtcg gacagcgtgc cggagatggc gtccgggacc gcaggcgggg
    10081 cgggtagctc cgcgctctgc ctgacggtca gggcggcggc gagcacgagg accgccgcgg
    10141 cggcggcgag cgccatccgg gagtgcctcg agccgggagc ggaggcgagc gtgcgggccg
    10201 cgacgcgggc ggcgagacgc gcgcgggcgc gggacacgcg cctcttcacc gccggaacgg
    10261 agatccgcag ggccgaggcc gcgtccgcca tgctcaggcc cgcggccgcc acgagcagga
    10321 tggcctcgcg gtcgcgcgac gagaggcgcg cgatcgcggc gcggacgcgt tccgcctcgt
    10381 cgcgtgcgag ggcccgggcc tccggcgtcg gcgcgtgcga gcgattcccg gccgcggatt
    10441 cgagcggcag ccacagccgc tgcctccagg acgcgcgcgc cgctctccgg cgcgcgatcg
    10501 cgaagacgaa tgcatcgggg gaggcggggg cgcctccgtt gcggcaggcg cggacgaggg
    10561 ccgcaaggct ctcctgcgcc acgtcctcgg cgagggctcg gtcgcccagc cgcgccgtcg
    10621 cgtagcgcag caccctcggc gcgagcgcga cgacggtgcc ttcgaggtcc acgatcgcaa
    10681 gagaaccctg gctcgccatg cggtcacact gagccgatca gatcgtgcat cgggtacgat
    10741 gaaacgtcac gcccggcggc gcctctcccg tggcccgccg ggcgggagga tgccctgtgc
    10801 gctgggtctt cgcgatcatc atcgctcttc acggcctgat tcacttcatg gggccagcca
    10861 aagcgttcgg ccttgccgac ctgccgcagc tcacgcgtcc gctctcgcgc ggcatgggac
    10921 tggcctggct gctggcgggt ctcctcacgc tcgcggccgt cgcggcgctc tttgcctggc
    10981 cgcggtgctg gtgggtgatc ggcgccgcag cggccgtcct ctcgcaggct gtcatcttca
    11041 cgtcctggac cgacgccagg gctggcacgc tcgccaacct catcctgctc gcgggggtgg
    11101 cctgcggcgt gctgacggga tggcccgggc gatcaccgtc atagccgggc ggcgcgccgc
    11161 cgaagaccct ggagactcgc acccgcctgc gggcgccccc gtcggacttc ccgaacctgg
    11221 ctcggctgcg cgggatgatc ggcgccggcg aggcttcatc tgcgcctgcg gtccggcccc
    11281 ggaccggagt agaatcacgg ccgactggag atgagcccat gagcgccagg aagccgcacc
    11341 gcgccccgac gcccgcgatg acgaggctcg acggcctgat cgcggctcca ttcacgccct
    11401 tcgaccggaa gggcgacgtg agcctgccgg tgatcgcgca gcaggtccgg cacctcctga
    11461 agaccggcgt ccggggcgcc tacgtgtgcg gcacgacggg cgagggcatt tcgtgcacgg
    11521 tggccgagcg gaaggccatc ctggaggagt gggtgcgccg gtcgggcggg cggctggtga
    11581 tcatcgcgca cgtcggcgcg ctctccgtgc gcgacgcgca ggaactggcg gcccatgcgc
    11641 aggcgtgcgg cgcggacgcg gcgtccatcg tgccgccgaa tttcttcaag ccggcgtcgg
    11701 tggccggcct cgtggcccac gtcgccgccg tggccgcggc ggcgccccgg ctgccgttct
    11761 actactacca cacgatgatg tcgggcgtgg acctgtcgat gccggcgttc ctcgaggccg
    11821 ccgagcgatc gatcccgacg ctggccggcg tcaagttcaa cagcttcaac ctctacgagt
    11881 accagaactg cctccgcgcg tgcggcggcc gctacgacat cgtctacggc gtggacgagg
    11941 tgtttgcggg cgccctgacg ctcggcgcgc gggcgttcat cggctccacg tacaactacg
    12001 cggcgccgct gtaccacgcc atccgggagg ccgtcgatgc cggccggctc gaggaggccc
    12061 gcgcgggcat ggcccgggtc tgcgccgtcg tggatctgct ggagcaacac ggcggtcttg
    12121 ccgcgggcaa ggccatgatg ggcgcccacg gcatcgacgt cggcgacccg cggctgccgc
    12181 tcggcgccct cgcccccgcc cgaaaggccg ccctcgtccg gcggcttcgg gagatcctcg
    12241 cctgagaacg cccgtgcgga cactcgcggc cggcatgcac gcctcgcggc ttgacgcgct
    12301 cgtccgcgtc taccgggacg gcctcctcca ggacaccgtg cccttctggc tgcgccacgg
    12361 cgccgatccc gagtacggcg ggatcatgac gtgcctcgac cgcgacgggt cgctcgtcga
    12421 caccgacaag ggcgtctggc agcaggggcg cttcgcctgg acgctcggcg tcctcgtcaa
    12481 caccgtggag cggcggccgg aatggctcgc cgcggccgag cacacgctca gattcatcga
    12541 ggcgcgctgc atcgacgcgg acggacggat gttcttccac gtcacgcgcg acgggcggcc
    12601 catccgcaag cgccgctacg cgttctcgga ggccttcgcg gcgatggcct tcggcgagca
    12661 cgcgcgcgcg accggccgtg cacgctcgcg cgacatggcc gagcgcctct acctcgccta
    12721 cgccgggcac gaaccgtcgc cgcccaagtg gcgcgacacg cgcccgatga agtcgatgag
    12781 cgggcccatg atccagattg ccgtgtgcca ggcgcttcgc gagtccatcg gcctggcgcg
    12841 cgccgacggg gacatcgacc gggccattgc cgaaatccgc cgcgacttca tgaagccgga
    12901 tctccggtgc tgcctggagc acgttgggcc ggacggcgcg gtgatcggcc acgcggacgg
    12961 ccgcctcctc aacccgggcc atgccatcga aggggcgtgg ttcctgatgc tcgagggcaa
    13021 aacgcgcggc atcggggagt acgtcgcggc cggctgcgac attctcgact ggatgtggga
    13081 gcgcggctgg gaccaggtgc acggcggcct cctctccttc gcggacctcg acggcaagcc
    13141 ggtggccgag tactggcacg acatgaagtt ctggtggccg cacaacgagg ccatcatcgc
    13201 cacgctgctc gcgtaccagc tgacgggcga cgcgaggtac gcgcgcctgc acgagcgggc
    13261 gcacgactac gcgtacgg
//
LOCUS       DAOSIF010000026        13158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4784_length_13158_cov_4.871632, whole genome shotgun sequence.
ACCESSION   DAOSIF010000026 DAOSIF010000000
VERSION     DAOSIF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4784_length_13158_cov_4.871632"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLT35_01895"
     CDS             <1..220
                     /locus_tag="PLT35_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOG28006.1"
                     /translation="VADAAVKAANVVLFRIHLAMAVGGKGFVVLTGDIASVEAAVAAG
                     AAVASGEGILVGKAVIPGPAKELFREFM"
     gene            432..3368
                     /locus_tag="PLT35_01900"
     CDS             432..3368
                     /locus_tag="PLT35_01900"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOG28007.1"
                     /translation="MHTRVWSRTAAASALACALVAAAAFGAAVILRAAQAPAPPAAPG
                     ASARPAAPSPPALADLRRQFVEPPDDARIMMRWWWFGPTVTKARLGREMRLMKEGGIG
                     GFEIQPVYPVVLDDAAQGLVTHPFLSDAFLDDLRFTAGRAKELGLRVDMTIGSGWPYG
                     GPQIGITQAAGTLRIERVPAPAGSMRVPVPDIRPGERLIAAFLAPSAPATSTKGLREA
                     SAIEDGVLRLPDRAASAREVLFFISSRTGQMVKRAAVGSEGFVLNHYDRAAVDHYLRE
                     VGDRLLALFGDAPPYAVFCDSLEVYGSDWTDGFLEAFRAKRGYDLRPLLPALLLDAGP
                     GTAGIRHDWGRTLTELLDERFYGPMQEWARRRHTKFRIQGYGIPPATILSNAAVDLPE
                     GEGAQWRVLRASRWASSIGHLYDRPVISSETWTWLHSPAFMASPLDLKAEADAHFLQG
                     INQLIGHGWPYTADGVAYPGWRFYAAGVYNEKNPWWIVMPDLSRYLQRLSWLLRQGRP
                     VNDVAFYLPTADTWAQFVPGRIDSFIDMMSRSLGPDIVAAVLDAGLNLDFVDDGVLDS
                     RAKAEQGRLVIGGNRYRAVVLPNVQRMPIATLRLLEEFAKQGGQVFATRRLPAEAPGY
                     MAAAADHAAVAAAAERLFRGASPAGVFVERDADLTAALRVRVAPDVAVSAGVGAVAAV
                     HRRTDAADIYFVANTSNTRQTLEATFRVASARAEAWNALTGEAVPVAVRPAGALRGKG
                     AAGASREGATVSLDLAPYESTVIVFPADTAGSRVRQPQTVRLPRQLPRPVDISAGWRV
                     AFGPNGAPSEWAALRSWTEAPETLHFSGVATYEKSVDVPAAMLGRGLSVRLDFGEPTP
                     REIGQPRYGTQAWLDAPVRDAAVVFVNGTRAGSVWCPPYAIDVTPFLKPGPNSLRVEV
                     ANLAINHMAGRALPDYKLLNLRYGSRFDPQDMDKVKPVPAGLLGPIRLVAAPAGPAAS
                     PGSAGR"
     gene            3780..5393
                     /locus_tag="PLT35_01905"
     CDS             3780..5393
                     /locus_tag="PLT35_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG28008.1"
                     /translation="MRVATLTLLLALAAMPAAAPQHPAPAPGPVDARLQALKQQALAE
                     VDSLRALTQQMIDQVYSFGELGFQEVETSRYLTGVLRKNGFAVTEGVAGMPTAWFASW
                     GSGKPVIALGSDIDGLPQTSQKPGIPWHEPLVDGAPGHGEGHNSGVPMNITAALAVKR
                     IMEREKLGGTIVVWPGVAEELVAAKAFYVRDGLFKDVDVVLFSHVASSMGVSWGAAAG
                     NGLVSVEYAFKGQSAHSAGMPWRGRSALDAVELMNAGWNFRREHLRLQQRSHYVITDG
                     GDQPNVVPSTAAVWYYFRETEYPRIMEMWQIGDRIAQAAAMMTDTTVTSRVLGAAWPQ
                     HFNKVVADVTYANIKTVGMPKWDEADQALARAVQRELKQAERGLESEVGRLGEPVRDE
                     DNRGGGSDDIGDVSWVVPTITLRYPANIPGTIGHHWSSGIAMATPIAHKGCTAGAKVH
                     ALTMLDLLLKPALVQQAWDYYTSVQTKDQKYTPIVRPGDKPATFLNAKLMAAYREKMR
                     PFYFDPSRYTTYLEQLGVPYPPHARPTDK"
     gene            complement(5449..6516)
                     /locus_tag="PLT35_01910"
     CDS             complement(5449..6516)
                     /locus_tag="PLT35_01910"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOG28009.1"
                     /translation="MSNSREHLAARLHGPRDLRVERVPHPGPPPAGHVLLRISLTGIC
                     GSDLHSYLDARIGDTEVAGPFVIGHEFSATVEGVGPGALDYACQPLRPGARVAVDPAQ
                     PCGVCELCARGHPNLCERLRFCGNCPDGGSLCEWMHMPARSCVPVPDALDDASAALLE
                     PLGVALHAVDLARIRVGDSAAILGAGPIGLLILQVARLAGASPIIVTDRLPARLALAE
                     RWGGIPIDIDRDDPAASVARLTGGRGVDVAIEAAWADESVSQAMDMLRLGGRAALVGI
                     PRDDRLTMRHSTARRKGATIRLCRRMKHAYPRALALALEGRVDLRPLVTHRFPLARAA
                     EAFGIAAAYADGVVKAVVESR"
     gene            complement(6550..8079)
                     /locus_tag="PLT35_01915"
     CDS             complement(6550..8079)
                     /locus_tag="PLT35_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOG28010.1"
                     /translation="MAVTRFRAFAAFVLAAWLSPAWVVIPAAAAAAPSPPNIVLVYAD
                     DLGFGDVGMNGSATVRTPNIDALAADGLRFTNAHAPAATCTPSRYSLLTGEYAWRRTG
                     TGVLPGDASLIIEPGRTTLPSLLRQAGYATGVVGKWHLGLGAGDVDWNRDIAPGPLDV
                     GFDYAFILPATGDRVPCVYVEDRRVVGLDPADPIRVSYRERVGDEPTGKARPDLLKLH
                     PSHGHDQTIVNGISRIGYMSGGAKARWVDEDIADTITAKAVSFIERHRARPFFLYFAT
                     HDIHVPRAPHPRFTGSSGMGPRGDAIVEFDWSVGEIVKALDRLGLASNTLLVVTSDNG
                     PVVDDGYRDQAVERLGAHRPEGPYRGGKYSAFEGGTRVPFVLRWPARVRRGVSGALVG
                     QIDLASSLAALTGQPVPPGAAPDSQNVLPALLGQSREGREWIVEQAGPLSLIKGRWKY
                     IEPRDGPRVSTSTNTELGNDPGPQLFDLEADPGERRNLAAAHPDRVAELAALLTRVRE
                     R"
     gene            8289..9449
                     /locus_tag="PLT35_01920"
     CDS             8289..9449
                     /locus_tag="PLT35_01920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOG28011.1"
                     /translation="MNLVIASDWAPIRAFDPIIRADPEAIYGDLLPILRDADLRIVNC
                     ECALTSAAKPVWKSGAVFKGAPAHAAGLTAVPFDVACLANNHVFDYGIAGFRESLRVL
                     ARAGIRTVGAGLTEAEAHAPLAVRTKRQHVHLVNISEGEDLTAARGGPGVVGWDVPRA
                     AALVRRLKTRGGVVIAIAHCGLEYVPFPAPYVVAAFRALVDAGADCVIGHHPHVPQGI
                     ELRRGCPIFYSLGNFAFYQPTDLHHRKVGFCVSLRCARGRIDGLELHPYRITDAGLRR
                     LDAAERRAFNRTMARLSRPFHTPSGPARAWNAWLEYYGQQGFRAEVTAILDRMDTDPR
                     KGAAMFRNRLTTAQHTEHWRTFLSRLIAGGRHDYAKADYKLVEEYFRKRAAP"
     gene            complement(9504..10487)
                     /locus_tag="PLT35_01925"
     CDS             complement(9504..10487)
                     /locus_tag="PLT35_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28012.1"
                     /translation="MRILLLTGPGGDAQGWGDMHVTESVAEAASAVGHPARIAFAETE
                     QDFLRAVDGGGFDIVWSALYYITANERFIGRGENAMWVADVLDARGIPYIGSDSRTMR
                     EMIDKYRTHEALAARGVAVPAHHLVRSSDDVSAVRYPAFVKPMGESRSIGINDDSVVT
                     NADELMRQVRWIEREFGQAALVEDFLPGDEFTTLVLGNGATRECLPGLVSVAPQHYGK
                     HKVLRADLRGVGLTKISLPPSRGDEAKVLATQAADAMNCLDHVRIDIKTDAGGALRVM
                     EVNGIPGLKPLKSWAPQIYTLYYRSPEGEMADYRNLIRAIIAAAGTRYGIA"
     gene            complement(10594..11595)
                     /locus_tag="PLT35_01930"
     CDS             complement(10594..11595)
                     /locus_tag="PLT35_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOG28013.1"
                     /translation="MAPGAHTVITDSGLGGLAVCAALERALRPSAAATPVRLTYVNAW
                     PFEGRGYNDLADVPARARVFDAALRQMAGLRPDRIVIACNTLSILYPHTAFSLSSGVP
                     VRGIIDAGVGVFGESLAEDAAATIVLAGTRTTIESGVHRDRLAARGTDPARIAAVPCH
                     GLAAAIERDVNGPSAARLIEECAARAAAAAPPGGTLLLGLCCTHYVYVAGRLEAALAA
                     KTGRLVRALDPGRRLVEALLAEQTDGSDRSDGLIAAEAGGSRGREAGGEDGAPGGGSA
                     GLADRSGPAGIGAPDGVAVRVMSKVELAEESRVAIARLVEPVSRATAEALLSYARLR"
     gene            11636..>13158
                     /locus_tag="PLT35_01935"
     CDS             11636..>13158
                     /locus_tag="PLT35_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG28014.1"
                     /translation="MAQTRGRTLPPARTLAAAIVLIAAVAAAQAPDQSQARAPAAQQP
                     AAPVDQPPSAQPPAQDPAQQRPPVFRAGVRVVRVDATVTGRGDQPVADLTPADFEVSE
                     DGVPQTVDQLQFIKLDGQRPAGDETSLEIRSREHGEAEASREDVRVFALFLDDYHIDR
                     APQVTMPLRKTLSLFIERLWPTDLVAIMEPLTPLSELRFTRSKSELQRIVNSFEGRQG
                     EIFPIKSPMEEAQLMRGDVRRVRAQVTLSALAALTVKLGGIKEGRKAIVFVSQGPPTF
                     FATREPDLQDQMREIFQAANRGNVTIYPLDPVGLTLEWRTGDKNTLYQLAADTGGRPI
                     VNTNNFSDGLNRVFEDSSAYYMIGYTPTRADDDGKYHKISVKVKRPGVHVIARQGYYA
                     PSTKELEAAAEASSRVLEPHVAGALQTLARQEQNKRPADIWTGLSRLPDNRTRVVVAW
                     DPVEPPPNVPVASLDVELLPAKNAAGQPAQSLPAPVAGGPPRRAAAFEADEGPVSLR"
BASE COUNT         1928 a         4715 c         4702 g         1813 t
ORIGIN      
        1 cgtcgccgac gcggcggtga aggccgcgaa cgtcgtgctc ttccgcatcc acctcgcgat
       61 ggccgtcggc ggcaagggct tcgtcgtgct gaccggcgac atcgccagcg tcgaggcggc
      121 cgtcgcggcg ggcgccgcgg tggcctcggg cgagggaatc ctcgtcggca aggcggtcat
      181 tccggggccg gcgaaggagc tgttccgcga gttcatgtga ggccacgccg gcggcccggc
      241 ggcggaccct ggctccgacg cgcgcggcct gtcggcgcac gatgccggta gcgttcccgg
      301 ggccctccgg gacaggccgg gcaggcagcg gccgtgcgcc ggggcgcagt cgcccttgtc
      361 ccgcgccggc gtccgaggca agctgacgca gccggaatga agttcgcccc gagggagggc
      421 ctcccgtgac catgcatacg cgagtctggt cccgcacggc ggcagcgtcg gcgctcgcgt
      481 gcgcgctcgt cgccgccgcc gcgttcggcg ccgccgtcat cctgcgcgcg gcgcaagcac
      541 cggctccgcc cgccgccccc ggcgccagcg ctcgcccggc cgcgccgtct ccgccggcgc
      601 tcgccgatct ccgccgccag ttcgtcgagc ccccggacga tgcgcgcatc atgatgcgct
      661 ggtggtggtt cgggccgacg gtgacgaagg cccggctcgg gcgggagatg cgcctgatga
      721 aggaaggcgg catcggcggg ttcgagatcc agccggtgta cccggtcgtg ctcgacgacg
      781 ctgcgcaagg gctcgtcacg catccgttcc tgtcggacgc cttcctggac gatctgcgct
      841 tcaccgcggg cagggcgaag gagctggggc tgcgcgtgga catgacgatc ggcagcggct
      901 ggccgtacgg cggcccgcag atcggcatca cgcaggcggc cggcacgctc aggatcgagc
      961 gcgtgcccgc tccggccgga tcgatgcgcg tgcccgtgcc ggacatccgt cccggcgagc
     1021 gcctgatcgc cgcgttcctc gcgccgtcgg ctccggcgac gtccacgaag ggcctgcgcg
     1081 aggcctccgc aatcgaggac ggcgtgctcc ggctgccgga ccgggcggcg agcgcccgcg
     1141 aggtgctgtt cttcatcagc agccgcacgg gccagatggt gaagcgcgcg gcggtcggtt
     1201 cggaggggtt cgtcctgaac cactacgacc gcgccgccgt cgatcactac ctgcgcgaag
     1261 tcggcgaccg cctcctcgcg ctcttcggcg acgcaccgcc gtacgccgtg ttctgcgaca
     1321 gcctcgaagt gtacgggtcg gactggaccg acggcttcct cgaggccttc cgcgcgaagc
     1381 ggggctacga cctgcggccg ctcctgcccg ctctcctgct cgacgcgggg ccgggcacgg
     1441 cgggcatccg ccacgactgg ggccggacgc tcaccgaact cctcgacgag cgcttctacg
     1501 ggccgatgca ggagtgggcg cggcgccgcc acacgaagtt caggatccag ggctacggca
     1561 tcccgccagc gacgatcttg agcaacgcgg ccgtcgatct ccccgagggc gagggcgcgc
     1621 agtggcgcgt gctgcgcgcg tcgcgctggg cctcgtccat cgggcacctg tacgaccgcc
     1681 ccgtcatttc gtccgagacg tggacgtggc tccactcgcc ggcgttcatg gcgtcgccgc
     1741 tggatctgaa ggccgaggcg gacgcgcact tcctgcaggg catcaaccag ctcatcggcc
     1801 acggctggcc ctacacggcc gacggcgtgg cgtacccggg atggcggttc tacgcggcgg
     1861 gcgtctacaa cgaaaagaac ccgtggtgga tcgtcatgcc cgatctttct cgctacctgc
     1921 agcggctgag ctggctcctg cgccagggcc ggcccgtgaa cgacgtggcg ttctacctcc
     1981 cgactgccga tacgtgggcg cagttcgtgc cgggcaggat cgacagcttc atcgacatga
     2041 tgtcccggtc gctcggaccc gacatcgtgg cggcggtgct ggacgcgggc ctcaacctgg
     2101 acttcgtgga cgacggcgtg ctcgactcga gagcgaaagc ggagcagggc cggctcgtca
     2161 tcggcggcaa ccgctaccgg gccgtcgtcc tgccgaacgt gcagcgcatg cccatcgcca
     2221 cgctgcgcct gctcgaggag ttcgcgaagc agggcggcca ggtgttcgcc acgcggcgcc
     2281 tgccggccga agcgcctggc tacatggccg cggcagcgga tcacgcggcg gtggccgccg
     2341 cggctgagcg gctgttccgc ggggcgtctc cggcgggggt gttcgtcgaa cgcgacgccg
     2401 atctcaccgc cgcgctgcgc gtgcgggtgg cgccggacgt ggcagtgtcg gccggcgtgg
     2461 gcgccgtggc cgcggtgcac cggcggacgg acgcggccga catctacttc gttgcgaaca
     2521 cctcgaacac gaggcagacg ctcgaggcca cgttccgcgt cgccagcgcc agggcggaag
     2581 cgtggaacgc gctgacggga gaggccgttc cggtggccgt acggcccgcc ggggcccttc
     2641 gcggcaaggg tgccgcgggc gccagccgag aaggcgcgac ggtgtcgctc gatcttgcgc
     2701 cctacgaatc gaccgtcatc gtctttcccg ccgacaccgc aggttcgcgc gtccgccagc
     2761 cgcagacggt gaggctgccg cgccagctgc cgcggcccgt cgacatcagc gccggatggc
     2821 gcgtggcatt cggcccgaac ggcgcgccgt cggagtgggc ggcgctgcgt tcgtggacag
     2881 aagctcccga gacgctgcac ttctcgggcg tggcgacgta cgagaagagc gtggacgtgc
     2941 cggcggccat gctcgggcgc ggcctctcgg ttcgcctgga tttcggcgag ccgacgccgc
     3001 gggagatcgg ccagccgcgc tacggcacgc aggcctggct ggacgcgcca gtgcgggacg
     3061 cggccgtcgt gttcgtgaac gggacgcggg cggggtctgt ctggtgcccg ccctacgcca
     3121 tcgacgtcac gcctttcctg aagccgggcc cgaacagcct gcgcgtcgag gtcgcgaacc
     3181 tcgcgatcaa ccacatggcg gggcgcgcgc tgcctgacta caagctgctg aacctgcggt
     3241 acggatcgcg gttcgatccg caggacatgg acaaggtgaa gccggtgccg gcggggctgc
     3301 tcggcccgat ccggctcgtg gccgcgccgg cggggcccgc ggcgtcgcca ggctccgcag
     3361 gccggtaacc ggcccacgaa gcaacgggct ctcgcggcgg gcctgacccc gggctctgct
     3421 cagggcgcag gcagaggcgc ggctggcgag gccgcctcgc gcaggaacga gccggccggc
     3481 gagtgcgagc gcaagcccgt tcccgaaccg ggctcctcgc tcttgctgct ttcgaccggc
     3541 ctcctcgccc tgcgcgcctc cagatcacgt gctcgtgagt ggttggcagt gggattgagc
     3601 cggcagcccc ttgccgtgtc aggcatcccg tgacttcgcg gtcgcgcagg agcgccgcat
     3661 cctgaagtcg agcatgtacg tgccgaccac gcgccgtccc gggcgcgcgg ctggagtaga
     3721 ctgtccgcgc ccggcctgac cgacgcccgt ccagggagga cccgccgatg agaacgccga
     3781 tgcgcgttgc cacgctgacc ctgttgctgg cgctggccgc gatgcccgcg gccgcgccgc
     3841 agcatcccgc gccggccccc ggccccgtcg acgcgaggct gcaggcgctg aagcagcagg
     3901 ccctcgccga ggtcgacagc ctgcgcgcgc tcacgcagca gatgatcgat caggtctaca
     3961 gcttcggtga actgggcttc caggaggtcg agacctcccg ctacctcacc ggcgtgctgc
     4021 gcaagaacgg gtttgccgtc acggaaggcg tggccggcat gccgacggcc tggttcgcgt
     4081 cgtggggatc gggcaagccc gtcatcgcgc tcggctcgga catcgacggc ctgccgcaga
     4141 ccagccagaa acccggcatc ccgtggcacg aaccgctcgt ggacggcgcc ccgggacacg
     4201 gcgaaggcca caattcgggg gtgccgatga acatcaccgc cgcgctggcg gtgaaacgga
     4261 tcatggagcg cgaaaagctc ggcggcacga tcgtcgtctg gccgggcgtc gccgaggagc
     4321 tggtggcggc caaggctttc tacgtccgcg acggcctctt caaggatgtg gacgtcgtcc
     4381 tgttctcgca cgtcgcctcg tcgatgggcg tgtcgtgggg cgcggcggcc gggaacgggc
     4441 tcgtgtcggt cgagtacgcg ttcaaggggc agagcgccca cagcgcgggc atgccgtggc
     4501 gcgggcggtc agcgctggat gcggtcgagt tgatgaacgc cggctggaac ttccggcgcg
     4561 agcacctgcg gctccagcag cgctcgcact acgtcatcac cgacggcggc gaccagccga
     4621 acgtcgtccc gtcgaccgcg gccgtctggt actacttccg cgagaccgag tacccgcgca
     4681 tcatggagat gtggcagatc ggcgaccgca tcgcccaggc ggcggcgatg atgaccgaca
     4741 ccaccgtgac ctcccgggtg ctcggggcgg cctggccgca gcacttcaac aaggtcgtcg
     4801 ccgacgtgac ctacgccaac atcaagaccg tgggcatgcc gaagtgggac gaggccgacc
     4861 aggcgctcgc cagggcggtg cagcgcgagc tgaagcaggc cgagcgcggc ctggagtccg
     4921 aggtcggccg gctcggcgag ccggtccgcg acgaggacaa ccgcggcggc gggtccgacg
     4981 acatcggcga cgtgtcgtgg gtcgtgccga cgatcacgct gcgctacccg gcgaacatcc
     5041 ccggcacgat cgggcaccac tggtcgagcg gaatcgccat ggcgacgccg atcgcgcaca
     5101 agggctgcac ggctggcgcg aaggtgcacg cgctcacgat gctggacctg ctgctgaagc
     5161 cagcgctcgt ccagcaggcc tgggactact acaccagcgt ccagacgaag gaccagaagt
     5221 acacgccgat cgtgcggccc ggggacaaac cggccacgtt cctcaacgcg aagctcatgg
     5281 cggcataccg cgagaagatg aggccgttct acttcgaccc atcgcggtac acgacgtacc
     5341 tggagcagct gggcgtgccg tacccgccgc acgccaggcc cacggacaag taacggccgt
     5401 tcgagacaag gcccgggcgc aggcgcttgc ggaccgccgg tccgaatttc agcggctctc
     5461 gacgacggcc ttcaccaccc cgtctgcgta ggccgcggca atcccgaacg cctcggcggc
     5521 ccgcgcgagc gggaagcggt gcgtcacgag gggccgcaag tcgacgcggc cttcgagggc
     5581 cagcgcgagc gcgcgcgggt aagcgtgttt catgcgccgg cacagccgga tggtcgcgcc
     5641 cttgcgccgc gcggtcgaat gccgcatcgt gagccgatcg tcgcggggga tgccgaccag
     5701 cgccgcgcgc ccgcccaggc ggagcatgtc catcgcctgt gagacggact cgtcggccca
     5761 cgcggcctcg atcgccacgt ctacgccgcg gccgcccgta aggcgcgcga ctgatgccgc
     5821 cgggtcgtcg cgatcgatgt cgatgggaat gccgccccag cgctcggcga gcgcgaggcg
     5881 cgcgggcaaa cggtcggtga cgatgatcgg cgaggcgccg gcaaggcgcg ccacctgcag
     5941 gatcaagagg ccgatgggcc cggcgccgag gatcgcggcg ctgtcgccga cgcggatgcg
     6001 ggcgagatcc accgcatgga gggcgacgcc gagcggctcg agaagcgccg cgctcgcgtc
     6061 gtccagcgcg tcgggcacgg gcacgcacga gcgcgcgggc atgtgcatcc actcgcacag
     6121 gctgccgccg tcgggacagt tgccgcagaa gcgcaggcgc tcgcacaggt tcggatgccc
     6181 gcgcgcgcac agctcgcaca cgccgcaggg ctgggcgggg tccaccgcga cgcgcgcgcc
     6241 gggcctgagg ggctggcagg cgtagtccag ggcgcccggg ccgacgccct cgaccgtggc
     6301 cgagaattcg tgaccgatga cgaacgggcc ggcgacctcc gtgtcgccga tgcgcgcgtc
     6361 gagatacgag tgcagatcgc tcccgcagat gccggtcagg ctgatccgaa ggagcacgtg
     6421 gcctgcgggt ggcggtccgg gatgaggtac ccgctcgacg cgcagatcgc gcggcccgtg
     6481 aaggcgcgcg gcgagatgct ccctgctgtt gctcatgatc cgggcctctg aagacccgcg
     6541 gtctgcgaat cagcgctccc gcaccctcgt caacagcgcc gccagctccg ccacgcggtc
     6601 cgggtgcgcc gcggcgaggt tccgccgctc accgggatcc gcctcgagat cgaacagctg
     6661 cggcccggga tcgttgccca gttcggtgtt cgtgctggtc gagacgcgcg gcccgtcgcg
     6721 gggctcgatg tacttccagc ggcctttgat cagggagagc ggaccggcct gttcgacgat
     6781 ccactcccgg ccctcgcggc tctggccgag gagcgcaggg agcacgttct ggctgtcggg
     6841 cgccgccccc ggcggaaccg gctgtcccgt cagcgccgcg agcgacgacg ccagatcgat
     6901 ctggccgacg agcgcgccgg acacgccgcg cctcacgcgc gccggccacc gcaggacgaa
     6961 gggcacccgc gtgccgccct cgaacgcgct gtacttgccg cccctgtacg ggccctcggg
     7021 ccggtgcgcg ccgagtcgct cgaccgcctg gtcccggtat ccatcgtcga cgaccgggcc
     7081 gttgtcgctc gtgacgacaa gaagcgtgtt cgaggcgagc ccgaggcggt cgagcgcctt
     7141 cacgatctcg ccgacgctcc agtcgaactc gacgatggcg tcgccgcgcg gccccatgcc
     7201 gctgctgccc gtgaaccgcg ggtgcggcgc gcgggggacg tggatgtcgt gcgtcgcgaa
     7261 gtagaggaag aacggccggg cccggtgccg ctcgatgaaa gagacggcct tcgcggtgat
     7321 cgtgtccgca atgtcctcgt ccacccacct cgccttcgcc ccgccgctca tgtagccgat
     7381 gcggctgatg ccgttgacga tcgtctgatc gtggccgtgg ctgggatgca gcttcagcag
     7441 atccggacgc gccttgcccg tgggctcgtc cccgacgcgc tcgcggtagc tcacgcgaat
     7501 ggggtccgcc gggtcgagac cgaccacgcg ccggtcttcg acgtacacgc acggcacgcg
     7561 gtcgcccgtc gccgggagga tgaacgcgta gtcgaagccg acgtccagcg gcccgggcgc
     7621 gatgtcgcgg ttccaatcca cgtcgcccgc ccccaggccg aggtgccact tgccgacgac
     7681 gcccgtggcg tagcctgcct gtcgcaggag tgagggcagc gtcgtgcggc caggctcgat
     7741 gatgagcgac gcgtcccccg gcagcacgcc ggtgcccgtg cggcgccacg cgtactcgcc
     7801 ggtgagcagc gagtaccggg acggggtgca cgtcgccgcc ggcgcatggg cgttggtgaa
     7861 ccggaggccg tcggcggcga gggcgtcgat gtttggcgtg cgaacggtcg cggagccgtt
     7921 catcccgacg tcgccgaagc cgaggtcgtc ggcgtagacg aggacgatgt tcgggggcga
     7981 gggggcagcg gcggctgcgg cggggatcac gacccacgcc ggcgagagcc acgcggcaag
     8041 aacgaacgcg gcgaacgcgc ggaagcgggt gactgccacg gcgccagtat acgcggtcga
     8101 cccgcaaaac ggccgcttcg ggagcttcaa cgcggtgctg aagggcacgg cgatccccca
     8161 cgaccaggag cagcgaagta gcggggtagc gagcagcggg gtcagatcca ggaaaacggg
     8221 gtcagatcca ggaaaacggg gccagggccg ggatcgcggc ataatgtccg ggtcctagac
     8281 ctgacctcat gaacctcgtc atcgcgtcag actgggcgcc gatacgcgcc ttcgacccga
     8341 tcatccgcgc agatccggag gcgatctacg gcgatctcct tccgatcctc cgcgatgccg
     8401 acctgcgcat cgtcaactgc gagtgcgccc tgacgtcggc agcgaagccg gtgtggaaga
     8461 gcggcgcggt gttcaagggc gcgcccgcgc acgccgcggg cctgacggcc gtcccattcg
     8521 acgtggcctg cctggccaac aaccacgtct tcgattacgg catcgcgggc ttccgcgaga
     8581 gcctgcgcgt gctcgcccgc gcgggcatcc ggaccgtcgg cgccggcctg accgaggcgg
     8641 aggcccacgc gcccctcgcc gtgcgcacga agcggcagca cgtccacctc gtcaacatca
     8701 gcgaaggcga ggatctcacg gcggcgcgcg gcggccccgg cgtcgtcgga tgggacgtcc
     8761 cgagggccgc ggcgctcgtt cgtcgcctca agacccgcgg cggcgtcgtg atcgccatcg
     8821 cgcactgcgg gctcgagtac gtgccgttcc cggcgccgta cgtggtcgcg gcgttccggg
     8881 cgctcgttga cgcgggggcg gactgcgtca tcggccatca cccgcacgtg ccgcagggga
     8941 tcgaactccg gcgcggctgc ccgatcttct acagcctcgg caacttcgcc ttctaccagc
     9001 ccaccgacct ccaccaccgg aaagtcggct tctgcgtcag cctgcgctgc gcccggggcc
     9061 ggatcgatgg gttggaactc catccctacc gcatcacgga cgcaggcctg cggcgcctcg
     9121 atgccgccga acgccgcgcg ttcaaccgga cgatggcccg gctctcgcgt ccgttccaca
     9181 cgccctccgg gcccgcccgc gcatggaacg cctggctgga gtactacggc cagcagggat
     9241 tccgcgcgga ggtgacggcc atcctcgaca ggatggacac ggacccgcgg aagggcgccg
     9301 cgatgttccg caaccgcctg acgacagcgc agcacacgga gcactggcgg acttttctct
     9361 cgcgcctgat tgccggcggc cggcacgatt acgcgaaggc tgactacaag ctggtggagg
     9421 agtacttcag aaagcgggca gcgccatgag ccggtccgcc gagtgaaggc ggccgcctgg
     9481 cgcctggccc gaaggccggc ccgtcaggcg atcccgtacc gcgtgcccgc agcggcgatg
     9541 atggcgcgga tgagattccg gtagtccgcc atctcgcctt ccggcgacct gtagtacagc
     9601 gtgtagatct gcggcgccca gctcttgagc ggcttgagcc ccgggatgcc gttgacttcc
     9661 atgacccgca gcgccccgcc ggcatcggtc ttgatgtcga tgcgcacgtg gtccaggcag
     9721 ttcatggcgt ccgcggcctg cgtcgccagc accttcgctt cgtcgcctcg cgacggcggc
     9781 aggctgatct tcgtcaggcc gaccccgcgc aggtccgccc gcagcacctt gtgcttcccg
     9841 tagtgctgcg gcgcgacgga cacgaggccg ggcaggcatt cgcgcgtcgc gccgtttcca
     9901 agcacgagcg tcgtgaattc atcgcccggc aggaagtcct cgacgagcgc ggcctggccg
     9961 aactcgcgct cgatccaccg cacctgccgc atgagctcgt cggcgttcgt cacgacgctg
    10021 tcgtcgttga tgccgatgga tcgcgattcg cccatcggct tgacgaacgc ggggtaccgg
    10081 accgcggaca catcgtccga gctgcgcacg aggtgatggg caggtacggc cacgccgcgc
    10141 gccgccagcg cctcgtgcgt ccggtacttg tcgatcatct cccgcatggt ccggctgtcg
    10201 gaaccgatgt agggaatccc tcgggcgtcg agcacgtcgg ccacccacat ggcgttctcg
    10261 ccccgcccga tgaatcgctc gttcgcagtg atgtagtaga gcgcgctcca gacgatgtcg
    10321 aagccgccgc cgtcgaccgc gcgcaggaag tcctgctcgg tttccgcgaa cgcgatgcgg
    10381 gccgggtggc ccacggcgga ggcggcctcg gcgaccgact cggtcacgtg catgtcgccc
    10441 cagccctgcg cgtcccctcc gggaccggtc aacagcagga tcctcatcga gcagctccag
    10501 gcgtgagaga tcggcgcccc agcaatcggg cctctcgtgc gatgacgaat tcgtcagtcg
    10561 cgggaccggt cgtgcctaaa tcgtcccgac cccttatcgg aggcgcgcat aagatagcag
    10621 ggcctccgcc gtcgcgcgcg acaccggctc gacgaggcgc gcgatcgcga cgcgcgattc
    10681 ctccgcaagc tcgaccttcg acatgacgcg aacggcgact ccgtccggcg caccgatgcc
    10741 tgccggtccg ctgcggtcgg ccaacccggc gctgccgccg cccggcgcgc cgtcttctcc
    10801 acccgcctcc cggccccggc tgcctccggc ctccgcggca atcaatccgt ccgatctgtc
    10861 cgacccgtcc gtctgctccg ccagcagcgc ctcaaccagg cgtcggccag gatcgagggc
    10921 ccgcaccagc cggcctgtct tcgcggccag cgcggcttcc agccgaccgg cgacgtagac
    10981 gtagtgcgtg cagcagagcc cgaggagcag cgtgccgccg ggcggggccg cggccgccgc
    11041 gcgggcggcg cactcttcaa tcagcctcgc ggccgacggg ccgttcacgt cccgctcgat
    11101 ggccgccgcc aggccgtggc acggcacggc ggcgatgcgc gcagggtcgg tcccgcgcgc
    11161 agccaggcgg tcccgatgca cgcccgattc gatggtagtc ctggtccccg cgaggacgat
    11221 ggtggcggcc gcgtcctcgg ccaggctctc gccgaacacg ccgacgccgg cgtcaatgat
    11281 gccgcgcacg ggcacgccac tgctcaggct gaacgccgtg tggggataca ggatcgacag
    11341 cgtgttgcag gcaatcacga tgcgatcggg ccggaggccc gccatctgcc ggagcgccgc
    11401 gtcgaacacg cgggcccgcg cgggcacgtc ggccagatcg ttgtatcccc ggccctcgaa
    11461 cggccacgcg ttgacgtagg tcagtctcac cggagtcgcg gcagccgacg gccgcagcgc
    11521 gcgctcgagc gcggcgcaga cggcgagccc gccgagtccg gagtccgtga tgacggtgtg
    11581 cgcgccaggg gccacgacgc gagtatagcg cccgcgccgg gactacaatg cgtgtatggc
    11641 ccagactcgc ggccgcaccc tcccgccggc gcgcaccctc gccgccgcga tcgtcctcat
    11701 cgcggcagtg gccgctgctc aagcgccgga ccagagccag gcacgagcgc cggcggcgca
    11761 gcaaccggcg gcccccgtcg atcagcctcc ttccgctcag ccgccggccc aggacccggc
    11821 gcagcagcgt ccgccggtgt tccgggcggg cgtgcgggtg gtgcgcgtcg acgcgacggt
    11881 caccgggcgc ggcgaccagc ccgtggccga tctgaccccc gccgacttcg aggtgagcga
    11941 ggacggcgtg ccccagacgg tcgatcagct ccagttcatc aagctcgacg gtcagcgccc
    12001 ggccggcgac gagacgtcgc tcgagatcag gtcgcgcgag cacggcgagg ccgaagcctc
    12061 gcgcgaggac gtccgcgtgt tcgccctctt cctcgacgac taccacatcg accgggcgcc
    12121 gcaggtcacg atgccgctcc gcaagaccct ctcgctcttc atcgagcggc tgtggccgac
    12181 ggatctcgtc gcgatcatgg aaccgctcac tccgctgagc gagctgcgct tcacgcgctc
    12241 gaaatccgag ctgcagcgga tcgtgaacag cttcgaaggg cgccagggcg agatcttccc
    12301 gatcaagagc ccgatggaag aagcgcagct gatgcggggc gacgtccgcc gcgtgcgggc
    12361 ccaggtcacg ctgtcggcgc tggccgcgct gacggtgaag ctgggcggca tcaaggaagg
    12421 ccggaaggcg atcgtcttcg tgagccaggg cccgcccacg ttcttcgcga cccgcgagcc
    12481 ggatctccag gaccagatgc gcgagatctt ccaggccgcc aaccgcggga acgtcacgat
    12541 ctatccgctc gacccggtgg gcctgacgct ggagtggcgg accggcgata agaacacgct
    12601 ctaccagctc gccgcggaca ccggcgggcg cccaatcgtc aacacgaaca acttctcgga
    12661 cggcctcaat cgggtgttcg aagacagcag cgcgtactac atgatcggct acacgcccac
    12721 cagggccgac gacgacggca agtaccacaa gatcagcgtg aaggtgaagc gccccggcgt
    12781 ccacgtgatc gcgcggcagg ggtactacgc gccgagcacg aaggagctgg aggctgccgc
    12841 cgaggcctcg tcgcgcgtgc tcgagccgca tgtcgccggc gcgctgcaga ccctcgcccg
    12901 ccaggagcag aacaagcgcc cggcggacat ctggacgggc ctgtcgcggc tgccggacaa
    12961 ccgcacgagg gtcgtggtgg cctgggaccc ggtcgagccg cctccgaacg tgccggtcgc
    13021 gagcctcgac gtcgagctcc tgccggcgaa gaacgccgca ggccagcccg cccagtcgct
    13081 gccggccccg gtcgcgggcg ggccgccgcg gcgggccgcg gcgttcgagg ccgacgaggg
    13141 cccggtctcg ctgcgctt
//
LOCUS       DAOSIF010000027        13027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4850_length_13027_cov_5.137296, whole genome shotgun sequence.
ACCESSION   DAOSIF010000027 DAOSIF010000000
VERSION     DAOSIF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4850_length_13027_cov_5.137296"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLT35_01940"
     CDS             complement(<1..724)
                     /locus_tag="PLT35_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG28015.1"
                     /translation="MEPQDPNDVPTAVDPENPEAALRDARQRFLAAFPKRSDSIGLML
                     STVATIGARGPVEPLRQVLHRMSGLAGMLGFPTVSSRARELELLLDGVGTGLFDAGRA
                     GLVFDALERAFTDDLASPPDWAGPSLSGGGSRRVMVIEDDEDQREVLGINLRAAGYEP
                     IDVVAGDLALDAVRAERPDVILLDANLPGIDGYTVCRLIKADPELAATPLIFLTVRAN
                     LDDRIAGLTLGADDYLVKPIDMP"
     gene            952..2073
                     /locus_tag="PLT35_01945"
     CDS             952..2073
                     /locus_tag="PLT35_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOG28016.1"
                     /translation="MHAGRTIGAALAVLVAAAVGMVWLTSPPAAAEQHAGPALREPGQ
                     PIERTLSVTASQGAEPAVAADQQGRDGRKTVITRLAQDAPMWAALTAVGPRLGVQIRD
                     VTADDVAKLKLPAQAGAVVEEVTKDSAAEKAGVKAGDVVVQYDGETVRSARQFTRLVR
                     ESVPDRTVKVVVLRDGRRVELNAAPAASAEPFGIRIDEEKLRDVEKKLGAAAERLQRF
                     RFERRPPAPDPDSPGGRVFRWRQPAPTPAPGGGMLDWLLQGGGSWMFAPGRGRLGVTV
                     QELTPDLADYFGVKDGLLVNSVQNDSPAARAGIRAGDVIQSVDGKAVTTANQLIGELA
                     DKNGDVTIGLMRDRKPLSVRAALEERPPLRRKAVIGKPA"
     gene            complement(2135..2509)
                     /locus_tag="PLT35_01950"
     CDS             complement(2135..2509)
                     /locus_tag="PLT35_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG28017.1"
                     /translation="MIGAREPLDPEIVAALRRAGEQLGNQELLAQLAGLFTDNAPLRL
                     EEIRGAIGRRDAPRAERAAHTLRTNCAVLGASELAACCGRLEEAAACADFDAASARLA
                     EAEDRLPDVLAAVTALAAEKSD"
     gene            complement(2516..3847)
                     /locus_tag="PLT35_01955"
     CDS             complement(2516..3847)
                     /locus_tag="PLT35_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28018.1"
                     /translation="MRSRGVRVLVALLAAAALAASGAAVWRAEQQLAAGQAAAEAFDH
                     DLRQVANSLGELRAAQFALVAHGQPADAWLSRSAALKESLVPSLESLHAETISAEGQG
                     ALEAAIEAFTAFGGQDARAREYLRSGQVLSASDVVFGGGAPLLDRVVGGLDMLRGQES
                     IARAIAAADARRRQLIAAGAGLAAILVALLLLVPVPRRAAAEIAGEALGAPPAGGLGL
                     SDRMEGFSREAPANAPPEGPWDQAKRVDRAVAESREDAPAPGPGGRAGHAGAEAQADL
                     PALADVCSRLARVQYSSELPGLLERASKALGASGMIVWLPDTHSGLLRPALSHGYTPA
                     ALARMGGIQPSADNAVAAAFLTKEIVTLRPDAFAKGAVVAPLHTAEGCSGALAVELMD
                     GIEPAPPIGAAAAIVAAQLATLLTPAAAEPASGSASAAPSAHRAATNAGKD"
     gene            complement(3844..4797)
                     /locus_tag="PLT35_01960"
     CDS             complement(3844..4797)
                     /locus_tag="PLT35_01960"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG28019.1"
                     /translation="MVLKVAVTLALYVAVFYKIDVRQFVERLQSADLGWLSLGVLVYA
                     GGQWLSAWRWWVLLRPVRLEVPFLRMVAFYFIGMFFNFFLPTIVGGDAVKAVLLSRET
                     GAPARATMSVFMERNVGLLALLAIATAAAMAAPRVAVKGVPLLHLTLVLFAAYLAANA
                     VLASRRAYGLIDRLVALTPLARTRSRAVSLHDALLPYRERQWWGTTAAATVQSVLFQG
                     VVILVVFLSAKALDERVPVSALAVFVPLISLAGMLPISVNGLGIREALYLLLFGRIGV
                     PADAAVSMALLYAAVTFAASLPGGLVYALQRGPRTGPGRST"
     gene            complement(4919..6670)
                     /locus_tag="PLT35_01965"
     CDS             complement(4919..6670)
                     /locus_tag="PLT35_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG28020.1"
                     /translation="MRHTRTAAFIAVVASAVSVAGAAAQLRRPEAALAPLVERPAGRA
                     GETVRLAVKVSLAEGLHTQSNAPRDPTLIPTALSIDAPAGITVSEVVYPKAVDFRQEG
                     QEQPLSVFEREFAIGVQVVVGPEALAGAAVPARLQYQACNDVMCFAPATAEFSWTIPP
                     AGEGAARSGAPDPVFASIAYGSGEKPAPTSPAAGPGATAAPATSPEQAAALLAALDRF
                     EVRGTAGGYLGTNAFLRFVRNAEAGVRERGVFEGRGPLAILVLVLLGGLALNLTPCVL
                     PMIPINIAIIGAGAQAGSRGRGFLLGGAYGTAMALVYGLIGLVVVLTAGTFGAINASP
                     WFNLAIAALFVALGLAMFDVFDIDFSRFGSRFRPGEGRGSLALAFTMGAVAALLAGAC
                     VAPVVIQVVLFSSNLYAAGTTVALALPFCLGLGMALPWPIVGAGIAAMPKPGRWMVRV
                     KQAFGIFILATAAYYAYQAYGLFATRWVDPADVKASVEEQLKTGWHAALADGLDEAAR
                     EGKPVLVDFWATWCKNCLTMDKTTFEEPQVKAALAGYVKIKVQAEDLDAQPAKDLLKR
                     FDAVGLPTYVILQPRWK"
     gene            complement(6684..8456)
                     /locus_tag="PLT35_01970"
     CDS             complement(6684..8456)
                     /locus_tag="PLT35_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG28021.1"
                     /translation="MDTVSLTIDGRGITVAKGTTILEAALENGISIPFFCYHPGIGID
                     GSCRVCLVRVAKMPKLQTACSTLCADGMVVETNSAEVVQARASVLEFLLINHPLDCPV
                     CDKGGECPLQDFSYAFGPEASRLEFPRRTFDGEGAAADVDFGPTLLLNRNRCIMCTRC
                     VRFMKIVDGDAQIGVVDRGGRSEIATFDERGVHSLLSGNLMDVCPVGAITTRDYRFAS
                     RPWDNPLAVDTTCTLCAKGCSTTVWLRAKPEWAKGARLIRITPRFNADVNGYWMCDIG
                     RFGHRWVGSPERLAAPLVRQASGAMAVATWPDALARLRAAARPALEAAPGSFRLLVSA
                     HASHEELFIVREITRRVPGLSASSVTVAWACSEKAQPKTTKFTVPAADAPNVAGAAAM
                     GLATPGSPAGRCDTGALRAAIDAGSVRALYVFDPGPAGSMGDLGWVLDARKSGALPLL
                     VVQGVLMTPLAAEADIVLPGSAWVEKEATYTNDRGLLQSTARALDPPGEAREDWRILA
                     DAAAALGARLDYADATAVRSAVAQEFQDVPALAAIGDAVFAPPVAIQTWLQASNPSER
                     WKWDRLFQARPPLKGFPPVTRAQE"
     gene            complement(8618..11371)
                     /locus_tag="PLT35_01975"
     CDS             complement(8618..11371)
                     /locus_tag="PLT35_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOG28022.1"
                     /translation="MPRRRAVPIAAAALALLMAGPGEVRTQQPLPLDGIVRLLLKIEQ
                     VMQAGNPDGYMDLLSSVAHRGLAREAAEALVVPGITRAVIRERDRGPLEGTLPGDGYR
                     LMVEIFTERGRQARVVTWRLDVRRVPTDTSGDEWRIAGQQALATVDGLSRLFLDPERQ
                     YRVRDFATRAHDLDVRMGEGLVFVASSAGGPTAAVFVPIRDGVIRFRPAPEVEREQLR
                     AYAGSDTIDTRFEALFLRLHPSDFRRRFPPETLAPEPVDPRVFRKAAEVFKESVPKSY
                     GLDLADLSRDVWSIEPQPGDFLAEIQTRRFQTLTYARSASDPEDISLFDRRRRRNISL
                     YASPERLAAGGRSFDEDGQAPYEVSHYQLDVSIDPERFWLDGRALVSLRVTANAINRL
                     TLRLAEPLTVRSVHSVEFGRLLALRVRGQNNVVVNLTGFATRGTILNLVVAYSGRLEP
                     DEPDRETAAPQFPQDTPILNEASSFPAETNLLYSTRSYWYPQASSGGYATATLHLAVP
                     EPYHVVASGELAPGSPVVVPQADRHQQGRLFTFEARQPVRYLACLISRFTRVDSRTVS
                     LVDAIGSVASRGSTALPSIPAGSGAFNGEIALTVQANPRQAGRGRNAAAVLAGVMEYY
                     ASLVGDAPYPALTLALIEKNLPGGHSPPYLAILNQPLPAAAISWSGDPAAFQGFPEFF
                     LAHEAAHQWWGQAVGWRNYHDQWISEGFSQYFAALWGRKSQGDGLFGQLLRRMAQWAR
                     KEGHAGPVSLGYRLGHIKDDSRIFRAIVYNKSATVLHMLRRMIGDDAFFRGVRHLYFE
                     FRFRKAGTDDVRRAFEQESGRSLKRFFDEWIDGFGTPLVAMTWSTRIAPAPAVTLRVE
                     QRGRLHEFPVTATIHYGDGRTEDHQIVAGDRVSEFSLPVSGDVRKVTLNRDGLTPLEI
                     APR"
     gene            11589..>13027
                     /locus_tag="PLT35_01980"
     CDS             11589..>13027
                     /locus_tag="PLT35_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG28023.1"
                     /translation="MNLHDARRRRRLSWEQAKRDLGYRDGDPLNLGRLCADRHVDEGR
                     GERVALIHEDHEGQVRRYTFRDLMELSNGWARFLSDQASVCQLDRVCLFLDRTPELYA
                     AFLGILKTGAIAEPLFPVFGEDALTARMLDSGAAAVITSRRHLGKLRRARSRLPDLRT
                     AVVVDAEPGGQGLRQGEMAFRMEDHRDARFSAAATHAESPSVLHYTSGTTGAPKGALH
                     VHGSVFAQYLTSKVVLDIRDDDVYWCTADPGWVTGTSYGIIGPWSLGATQVVLDGGFS
                     SERWYGTIERHRVSVWYTAPTAIRMLMKDGLDPVRRRDLSSLRHACSVGEPLNPEAVR
                     WGREAFGLWFHDTFWQTETGAIVVTNLPGMPVKPGSMGRPFPAVEAAIVEPGTGVPIA
                     EAGRVGLLAIRPPWPSLMRAYWNNTAAYYGKFLNGWYVSGDRASVDDDGYFWFCGRDD
                     DVINTAGHLVGPFEVESALLEHPAVAESAA"
BASE COUNT         1988 a         4563 c         4715 g         1761 t
ORIGIN      
        1 ccggcatgtc gatgggcttg accaggtaat cgtcggcgcc gagcgtgagc ccggcgatgc
       61 ggtcgtcgag gttcgcgcgc acggtcagga agatcagcgg cgtcgcggcg agctccgggt
      121 cggccttgat cagccggcag acggtgtagc cgtcgatgcc gggcagattg gcgtcgagca
      181 ggatcacgtc cggccgctcc gcccggacgg catcgagggc gagatcgccg gcgaccacgt
      241 cgataggctc gtaaccggcc gcccggaggt tgatgcccag cacctcgcgc tggtcttcgt
      301 cgtcttcgat caccatgacg cgccggctgc cgccgcccga gagggacggc cccgcccagt
      361 ccggagggct ggcgagatcg tccgtgaagg cccgctcgag ggcgtcgaac accaggccgg
      421 cgcggcccgc gtcgaacagc ccggtgccga cgccgtccag gaggagctcg agttcgcgcg
      481 cgcgggagga gacggtcggg aaccccagca tgcccgccag gccggacatc cggtggagca
      541 cctgccggag cggttccacc ggcccgcgcg cgccgatggt ggccaccgtc gagagcatca
      601 agccgatcga atccgatcgc ttcggaaaag cggccagaaa ccgctgtcgc gcatcgcgca
      661 gcgcggcctc cgggttctcg ggatcgacag ccgtcggcac gtcgttcggg tcctgtggct
      721 ccatgcacac cccttcccga cgccggacga gtcaagtccg ggtcggtgcg ccggtggctc
      781 cgccgggcgg acgcgcggcg ctctagcggg atatcggccg aacccgagtg tccggccaga
      841 gacggcgcgg ccggccgggc gctggcggag ggccctggcg accccaacca accgcgcctg
      901 ccccgcgtct gagtaggtgt agcgcggcga gttcttcgcc tggagggtgc tatgcacgca
      961 ggacggacga taggcgcggc cttggcggtc ctcgtggccg ccgccgtggg catggtgtgg
     1021 ctgacgtcgc cgccggccgc ggcagaacag cacgccggac cggccttgcg ggagcccgga
     1081 caaccgatcg aacgaacgct cagcgtcacc gcgtcgcagg gcgctgaacc ggcggtcgcg
     1141 gcggaccagc agggccggga cggccggaag accgtcatca cccgcctcgc gcaggacgcg
     1201 ccgatgtggg cggccctgac ggccgtcggc ccgcggctcg gcgtgcagat ccgcgatgtc
     1261 acggccgacg atgtggcgaa gctgaagctg ccggcccagg caggggccgt ggttgaagag
     1321 gtcacgaagg attccgccgc cgagaaggcg ggcgtgaagg ccggcgacgt ggtggtgcag
     1381 tacgacggcg agaccgttcg cagcgcccgc cagttcaccc gcctcgttcg cgagtccgta
     1441 ccggatcgca cggtgaaggt ggtagtgctc cgagacggaa ggcgcgtgga gctgaacgcc
     1501 gcgccggcgg cgtcagccga gcccttcggc atccggatcg acgaagagaa gctgcgagat
     1561 gtcgagaaga agcttggggc cgccgccgag cgactgcagc gtttccgatt cgagcgcaga
     1621 ccgcccgccc ctgacccgga ctcaccgggc ggccgggtct tccgctggcg gcagccggcg
     1681 ccgaccccgg cacctggtgg cggcatgctg gactggctcc tccagggcgg cggcagctgg
     1741 atgttcgcgc cggggcgcgg taggctcggc gtgacggtgc aggagctgac gcccgatctg
     1801 gctgactact tcggggtgaa ggacggcctg ctcgtgaaca gcgtgcagaa cgacagcccg
     1861 gcggcaaggg ccggcatcag ggccggggac gtgatccagt ccgtcgatgg caaggccgtc
     1921 accacggcga accagctcat cggcgagctg gctgacaaga acggcgacgt gacgatcggc
     1981 ctgatgcgcg acaggaagcc gctgtcggtc agggcggctc tcgaagagcg gccgccgttg
     2041 cgacgcaaag ccgtcatcgg caagccggcg taagggacgc gcgagatccg aaccgcgggg
     2101 cggcagctga agcgcgccgc gcgcgtccgg gctctcagtc gcttttctcg gccgccagcg
     2161 cggtgacggc cgccagcacg tccggcagcc ggtcttctgc ctccgccaga cgcgcggaag
     2221 ccgcgtcgaa gtcggcgcag gccgccgctt cctccagccg gccgcagcag gccgccaact
     2281 ccgacgcgcc aagcacagcg cagttcgtcc tgagcgtgtg ggcggcgcgc tcggcgcgcg
     2341 gcgcatcgcg gcggccaatc gcgccgcgga tttcctcgag ccgcagggga gcgttgtccg
     2401 tgaacagccc ggcgagctgg gcgagcaact cctggttgcc gagctgttct ccggcgcgcc
     2461 gcagcgctgc aacgatctcg ggatcgagcg gctcgcgcgc gccgatcacg ggccgtcagt
     2521 ccttccccgc gttcgtggcg gcgcggtgcg cggaaggagc agcagaggcg gatccggaag
     2581 ccggctcggc ggccgccggc gtgagcagcg tcgcgagctg ggccgccacg atcgcggctg
     2641 ccgcgccgat gggtggcgcc ggctcgatgc cgtccatcaa ctccaccgcc agcgcgccgg
     2701 aacacccttc ggccgtgtgg agcggggcca ccacggcgcc cttggcaaag gcgtcgggcc
     2761 gaagcgtcac gatttccttc gtgagaaacg ccgccgccac ggcgttgtcg gcggacggct
     2821 ggatgcctcc catccgggcg agcgcggccg gcgtgtagcc gtgcgaaagc gccgggcgga
     2881 ggaggcctga gtgcgtgtcg ggcagccaga cgatcatgcc cgacgcgccg agggccttcg
     2941 aggcccgctc gaggagaccg ggcaactcgc tcgagtactg tacgcgggcc agacgcgaac
     3001 acacgtcggc aagcgctggg agatcggcct gcgcctccgc accggcgtgc ccggcgcggc
     3061 ccccggggcc tggcgccggc gcgtcttccc gggattcggc gactgcccga tcgacgcgct
     3121 ttgcctggtc ccacgggccc tccggtgggg cgttggccgg cgcctcgcgg ctaaacccct
     3181 ccatccggtc agacaagccg aggccgccag ccggcggggc gccgagcgcc tcgccggcga
     3241 tctccgccgc cgcgcggcgc ggaaccggca cgagcaggag cagcgcgacc aggatggcgg
     3301 cgaggcccgc tcccgccgcg atgagctgcc gacggcgcgc gtcggcggcg gcaatggcgc
     3361 gcgcgatgct ctcctggccc ctgagcatgt cgaggcctcc gactacacga tcgagcaggg
     3421 gcgcgcctcc gccgaacacc acgtcggagg ccgagaggac ctggcccgac ctcaggtact
     3481 cgcgggcccg ggcgtcctgc ccgccgaatg cggtgaacgc ctcgatggcc gcttcgagcg
     3541 cgccctggcc ttcggcgctg atcgtctccg cgtgcagaga ctcgaggctc gggacgaggg
     3601 actccttgag ggcggccgat ctggacagcc acgcgtcagc cggctggcca tgggcgacga
     3661 gcgcgaactg ggcggcgcgc agctcgccga ggctgttggc cacctgccgc aggtcgtggt
     3721 cgaaggcttc ggcggcggcc tggccggcgg ccagttgctg ctcggcgcgc caaacggctg
     3781 cgccagaggc agccagcgcg gcggccgcca gcagcgcgac gagtacccgt acgccccgac
     3841 tcctcatgtg gacctcccgg gccccgtccg cggcccgcgc tgaagcgcgt agacgagccc
     3901 gccgggcagg ctggctgcaa aggtcacggc ggcgtagagc agcgccatcg acaccgccgc
     3961 gtccgccggc acgccgatgc gcccgaacag cagaaggtac aacgcttcgc ggatgccgag
     4021 gccgttcacg ctgatcggaa gcatgcccgc cagcgagatc agcgggacga acacggcgag
     4081 agcggagacg ggcacgcgct cgtcgagcgc cttggcgctc aagaatacca cgaggatgac
     4141 gacgccctgg aaaaggaccg actggaccgt cgcggccgcc gtcgtgcccc accactgccg
     4201 ctcgcggtac gggagcaggg cgtcgtggag ggagaccgcg cgggaccggg tccgcgcaag
     4261 cggcgtcagc gcgacgaggc ggtcgatgag gccgtatgcg cggcggctcg ccaacacggc
     4321 gttggcggcg aggtaggcgg cgaagaggac gagcgtcagg tgcaggagcg gcacgccctt
     4381 cacggcgacc cgcggcgcgg ccatcgccgc cgctgtcgcg atcgcgagca gcgccagcag
     4441 ccccacgttg cgctccatga acacgctcat cgtcgcgcgg gccggcgcgc ctgtctcgcg
     4501 cgagagcaga acggccttca ccgcatcgcc gccgacgatg gtcggcagga agaagttgaa
     4561 gaacatgccg atgaagtaga acgcgaccat ccgcagaaaa ggcacctcga ggcggactgg
     4621 gcgcagcagc acccaccacc gccaggcgct gagccactgg ccgccggcgt agaccaggac
     4681 gccgaggctg agccagccga ggtcggcgct ctgcaggcgc tcgacgaatt gccgcacgtc
     4741 gatcttgtag aaaacagcga cgtagagcgc cagcgtgacc gccaccttca acaccacacg
     4801 gggtcgggac gaattacgca cgcagcgctc ggtagacgac aaaactgtaa ccaggcagag
     4861 ccgatggcga gaatcgtaat cgcctgaagc ctccgcgcat aaatcgtccc gacccctttc
     4921 acttccagcg aggctgaagg ataacgtagg ttgggaggcc gacggcgtcg aaccgcttca
     4981 acaggtcctt cgccggctgc gcgtcgaggt cttcggcctg gaccttgatc ttcacgtacc
     5041 ccgcgagtgc cgccttcacc tgcggctcct cgaaggtcgt cttgtccatg gtcaggcagt
     5101 tcttgcacca ggtggcccag aagtccacca gcacgggctt gccttcccgc gcggcttcgt
     5161 ccaggccgtc cgccagcgcc gcgtgccagc cggtcttgag ctgctcctcg acgctggcct
     5221 tcacgtcggc cggatcgacc cagcgggtcg cgaagaggcc gtaggcctgg tacgcgtagt
     5281 aggcggcggt cgcgagaatg aagatcccga aagcctgctt cacgcgcacc atccagcggc
     5341 caggcttcgg catcgccgcg atgccggcgc ctacgattgg ccagggcagc gccatgccca
     5401 gaccgaggca gaacggcagc gcgagggcga ctgtggttcc ggcggcatac aggttgctcg
     5461 agaacagcac cacctggatc accaccggcg cgacgcaggc gccggccagc agcgcggcca
     5521 ccgctcccat tgtgaacgcg agcgcgagcg agccgcggcc ctcgcccggc ctgaagcggc
     5581 ttccgaaccg ggagaagtcg atgtcgaaca cgtcgaacat ggccaggccg agcgcaacga
     5641 agagcgcggc gatggccagg ttgaaccacg gcgacgcgtt gatggcgccg aaggtgcccg
     5701 ccgtcaagac caccacgagc ccgatgagcc cgtacaccag cgccatggcc gtcccgtacg
     5761 cgccgccgag caggaaccct cggccgcgcg agcctgcctg ggcgccggcg ccgataatcg
     5821 cgatgttgat cggaatcatc ggcaacacgc agggcgtcag attgagcgcc agcccgccga
     5881 gcaggacgag gaccaggatc gcgagcgggc cccggccctc gaatacgccg cgctcgcgca
     5941 cgcccgcctc ggcattgcgg acgaacctca ggaacgcgtt cgtgccgagg tagccgccgg
     6001 ccgtgccccg cacctcgaag cggtcgagtg cggcgagcaa ggcggcggcc tgctcgggcg
     6061 acgttgccgg tgccgctgtt gcgccaggcc cggccgcggg ggacgtcggc gccggcttct
     6121 cacccgagcc atacgcgatc gatgcaaaca ccgggtccgg cgcgccgctc cgcgcggcgc
     6181 cttcgccggc gggagggatg gtccacgaga actcggccgt cgccggcgcg aagcacatca
     6241 cgtcgttgca ggcctggtac tgcagcctgg ccggcacggc agcgccggcc agggcttcgg
     6301 ggccgacgac cacctgcacg ccgatggcga actcgcgctc gaacacggaa aggggctgct
     6361 cctggccctc ctgcctgaag tccaccgcct tcggatacac gacctcgctg acggtgatgc
     6421 cggcaggcgc gtcgatggac aaagccgtcg gaatgagcgt cgggtcgcgc ggcgcgttcg
     6481 actgggtgtg caggccctcg gcgagcgaca ccttcacggc caggcggacg gtctctccgg
     6541 cgcgtccggc cggccgctcg acgagcggcg cgagggcggc ttcgggacgc cgaagctgcg
     6601 cggccgcacc ggccacggaa accgcacttg cgacaacggc gatgaacgcc gcagttcggg
     6661 tgtggcgcac gttcgtcctt ctcctactcc tgcgctctcg tcaccggcgg gaatcccttg
     6721 agcggcgggc gggcctggaa caggcggtcc catttccagc gctcggaggg gttcgaggcc
     6781 tggagccacg tctggatcgc gacgggcggg gcgaagacgg cgtcgccgat cgccgcgagc
     6841 gccggcacgt cttgaaactc ctgcgcgacg gccgagcgca cggccgtggc gtccgcgtag
     6901 tccagtctcg cgccgagcgc cgcggccgcg tctgccagga tccgccagtc ctcccgggct
     6961 tctcctggcg ggtcgagcgc ccgcgctgtc gactgcagca ggccgcgatc gttcgtgtac
     7021 gtcgcttcct tctcgaccca tgcggatccg ggcaggacaa tgtcggcctc cgccgcgagc
     7081 ggcgtcatca gcacgccctg caccacgagc agcgggaggg cgcccgactt gcgcgcgtcg
     7141 aggacccagc ccagatctcc catgctccca gccggccccg gatcgaacac gtagagcgct
     7201 ctgaccgagc cggcgtcgat cgctgcgcgc agggcgccgg tatcgcagcg cccggcaggg
     7261 ctgccgggcg tcgccaggcc catcgcggcc gccccggcga cgttcggggc atcggccgcg
     7321 ggcacggtga acttcgtcgt cttgggctgc gccttctcgg agcaggccca cgccaccgtc
     7381 acgctcgacg cggagagccc cggcacgcgc cgcgtgatct cccgcacgat gaacagctct
     7441 tcgtgcgacg cgtgcgcgga caccagcagc cggaacgatc ccggcgcggc ctcgagggcc
     7501 ggccgcgccg ccgcgcgcaa gcgggcgagg gcgtccggcc aggtcgccac cgccatggcg
     7561 cccgacgcct gcctgacgag cggcgcggcg aggcgctcgg ggctcccgac ccagcggtgc
     7621 ccgaacctgc cgatgtcgca catccagtag ccgttcacat ccgcgttgaa gcgcggcgtg
     7681 atgcggatca gccgcgcgcc cttggcccac tcgggcttcg cccgcagcca caccgtcgtg
     7741 ctgcagccct tcgcgcagag cgtgcacgtc gtgtcgacgg ccagcgggtt gtcccacggc
     7801 cgcgacgcga agcggtagtc gcgcgtagtg atggcgccca cggggcagac gtccatgagg
     7861 ttgcccgaga gcagcgagtg cacgccgcgc tcgtcgaagg ttgcgatctc gctccggcct
     7921 ccccggtcca ccaccccgat ctgcgcgtcg ccgtcgacga tcttcatgaa gcggacgcag
     7981 cgcgtgcaca tgatgcagcg gttccggttc agcaggagcg tgggcccgaa gtccacgtcc
     8041 gccgcggcgc cctcgccgtc gaaggtgcgc cgcgggaact ccagccggct cgcctccggg
     8101 ccgaaggcat aagagaagtc ctggaggggg cactcgccgc ccttgtcgca caccgggcag
     8161 tcgaggggat ggttgatgag caggaactcc aacacgctcg ccctcgcctg gacgacttcc
     8221 gcgctgttcg tctcgaccac catgccgtcc gcgcacagcg tcgagcacgc cgtctgcagc
     8281 ttcggcatct tcgccacccg cacgaggcag acgcggcagg atccgtcgat gccgatcccc
     8341 gggtggtagc agaaaaacgg gatggagatg ccgttctcga gggccgcctc gagaatggtc
     8401 gtgcccttgg cgacggtgat gccgcgcccg tcgatcgtca gggaaacggt gtccatgggg
     8461 aaatcatacc gggattcggg actggagacg cgtcctgccg cttcgaagct ggcgggaagg
     8521 ccgtccggcg aagccgggcg ccgcgggccg ggcgcctcag gccgggcgcc gcgggaaagg
     8581 cgcaggagtc caggcggcgg aggggcagcg aaggcgacta gcgcggggcg atctccagcg
     8641 gggtgaggcc gtcgcggttc agcgtgacct tccgcacgtc gcccgacacg ggcaggctga
     8701 attccgacac gcggtcgccg gccacgatct ggtggtcttc cgtcctgccg tcgccatagt
     8761 ggatcgtggc cgtcaccggg aactcgtgga gccggccccg ctgctcgacg cgcagcgtca
     8821 ctgcgggcgc cggcgcgatc cgggtcgacc acgtcatcgc gaccagcggg gtcccgaagc
     8881 cgtcgatcca ctcgtcgaag aaccgcttca gcgaacgccc gctttcctgc tcgaaggccc
     8941 tgcgcacgtc gtccgtgccg gccttcctga agcggaactc gaaatacagg tgcctgacgc
     9001 cgcggaagaa cgcgtcgtcg ccgatcatgc gccggagcat gtgcaggacc gtcgccgact
     9061 tgttgtagac gatggcccgg aagatccggc tgtcgtcctt gatgtggccg aggcggtacc
     9121 cgagcgacac cgggcccgcg tggccttcct tgcgggccca ctgcgccatg cgccggagca
     9181 actggccgaa cagcccgtcg ccctggctct tgcggcccca gagagcggcg aagtactggg
     9241 agaagccttc gctgatccac tggtcgtggt agttgcgcca gcccacggcc tggccccacc
     9301 actggtgggc cgcctcgtgc gccaggaaga actccgggaa gccctggaag gccgccgggt
     9361 ctccgctcca gctgatggcc gccgccggaa gcggctggtt caggatggcg aggtacgggg
     9421 ggctgtgccc gcccggcagg ttcttctcga tgagcgcgag cgtgagggcg ggatacggcg
     9481 cgtcgcccac gagcgacgcg tagtactcca tcacgccggc gagcaccgcc gcggcgttcc
     9541 ggccgcggcc ggcctggcgg ggattcgcct ggaccgtcag agcgatttcg ccgttgaacg
     9601 ccccggaccc cgcggggatt gagggcaggg ccgtggaacc ccgggacgcc acgctcccga
     9661 tggcgtccac gagcgagacg gttctcgagt ccacccgcgt gaagcggctg atcaggcacg
     9721 cgaggtaccg caccggctgc cgcgcttcga acgtgaacag ccgcccctgc tggtgccgat
     9781 ccgcctgcgg cacgaccacc ggcgatcccg gcgccagctc gccgctcgcc acgacgtggt
     9841 acggctccgg gacggccagg tgcagcgtcg ccgtggcata tccgccggac gacgcctgcg
     9901 ggtaccagta gctgcgcgtc gagtacagca ggttcgtctc cgcggggaac gacgacgcct
     9961 cgttgaggat cggcgtgtcc tgcgggaact ggggggccgc ggtctcgcgg tccggctcgt
    10021 ccggttcgag ccggccggaa taggcgacga ccaggttgag gatcgtgccg cgcgtggcga
    10081 atccggtcag attgaccacg acgttgttct gcccgcggac gcgcagcgcg agcaggcggc
    10141 cgaactcgac gctgtggacg gaccgcaccg tcagcggctc ggccagccgg agcgtgaggc
    10201 ggttgatggc gttcgccgtg acgcggaggc tgacgagcgc ccggccgtcc agccagaacc
    10261 gctcgggatc gatcgacacg tcgagctgat agtggctgac ctcgtaggga gcctgtccgt
    10321 cctcgtcgaa cgatcgtccg ccagcggcca gacgctccgg cgacgcgtac aacgagatgt
    10381 tccggcgccg ccttcggtcg aacagcgaga tgtcttccgg gtcgctggct gaccgggcgt
    10441 atgtgagcgt ctggaagcgc cgcgtctgga tctccgcgag gaagtccccg ggctgcggct
    10501 cgatggacca gacgtcgcgg ctcaggtcgg ccaggtccag gccgtaggac ttagggacgc
    10561 tctctttgaa cacctccgcg gccttgcgga agacgcgcgg atcgacaggc tccggcgcga
    10621 gcgtctcggg cgggaaccgc cgccggaagt ccgacggatg gaggcgcagg aagagcgcct
    10681 cgaagcgcgt gtcgatcgtg tcgctcccgg cgtaggcccg aagctgctcg cgctcgacct
    10741 cgggtgccgg ccggaaccgg atcacgccgt cccggatcgg cacgaacacg gcggccgtcg
    10801 ggcctccggc gctcgacgcc acgaagacca ggccctcgcc catgcgcacg tccaggtcgt
    10861 gggcccgcgt cgcgaagtcc cgcaccctgt actggcgttc cgggtccagg aacaaccggc
    10921 tcaacccgtc cacggtcgcc agggcctgct ggcccgcgat tcgccactcg tcgccgctcg
    10981 tgtcggtcgg cacccgccgg acgtcgagcc gccaggtgac gacacgggcc tggcggccgc
    11041 gctcggtgaa gatttcgacc atgagccggt agccgtcgcc tggcagcgtg ccctcgagag
    11101 ggccgcggtc gcgctcgcgg atgaccgcgc gggtgattcc cggcacgacc agcgcctcgg
    11161 cggcttcacg cgccagtccc ctgtgggcca cgctcgacag gaggtccatg tagccgtccg
    11221 ggttgccggc ctgcatgacc tgttcgatct tcagcaggag gcgcacgatg ccgtcgaggg
    11281 gaagaggctg ctgggtccgc acctctcccg gcccggccat cagcagggcc agcgcggcgg
    11341 cggcgatcgg aacggctcgg cggcgaggca tcgaggggcc ttgacggctc actacattag
    11401 acgcgcgcgc gcgcccgttg gtgcttgggc tggttcccgt cgcgctcatt atccgcccgt
    11461 cctgcccgcc tggattgcct tcctgcgggc cgccccgcct ggcggccggg cgcgccttcc
    11521 gtctcgaggg ccaggccccg ggtgcagggc cgaacgggcg gaaccgataa ccggacacgg
    11581 agaccctcat gaacctccat gacgcacgcc ggcggcgccg cctgagctgg gagcaagcca
    11641 agcgcgacct gggataccgc gacggcgatc cgctgaacct cggccgcctc tgcgcagatc
    11701 gccacgtcga cgagggccgc ggcgaacgag ttgcgctcat ccacgaagac cacgaaggcc
    11761 aggtccgccg ctacaccttc cgcgacctga tggaactcag caacggctgg gcgaggttct
    11821 tgtcagatca ggcgtccgtt tgccagttgg atcgggtctg tcttttccta gataggacgc
    11881 ccgagctgta cgcggccttc ctgggcattc tgaagacagg ggcgattgcc gaaccgctgt
    11941 tccctgtgtt cggcgaggat gcgctgacgg cccgcatgct ggacagcggg gcggcggccg
    12001 tcatcacctc gcggcgccac ctcggcaagc tgcgccgggc ccgctctagg ctgccggacc
    12061 tgaggacggc cgtcgtcgtc gacgccgagc cgggcgggca agggctccgc cagggtgaga
    12121 tggcgttccg gatggaggac catcgcgacg cgcggttttc cgcggccgcg acgcacgccg
    12181 agagcccgag cgtgctgcac tacaccagcg ggacgaccgg cgccccgaag ggcgccctgc
    12241 acgtgcacgg cagcgtgttc gcgcagtacc tgacgtcgaa agtcgtgctg gacatccggg
    12301 acgacgacgt ctattggtgc acggcggacc cgggctgggt cacgggcacg agctacggca
    12361 tcatcggtcc gtggtcgctc ggcgccacgc aggtcgtgct cgacgggggg ttctcgagcg
    12421 agcgctggta cgggacgatc gagcggcacc gggtgagcgt gtggtacacg gcgccgacgg
    12481 caatccggat gctgatgaag gacggcctcg atccggttcg gcggagggat ctctcgagcc
    12541 tgcggcacgc gtgcagcgtc ggcgagcccc tgaatccgga ggcggtgcgg tggggccgcg
    12601 aggcattcgg cctctggttc cacgacacct tctggcagac cgagaccggg gccatcgtgg
    12661 tcacgaacct gcccggcatg cccgtgaagc ccgggagcat ggggcggccg tttccggccg
    12721 tcgaggcggc cattgttgag cccgggaccg gcgtgccgat tgcggaggcc ggccgcgtcg
    12781 gcctgctggc gatccggccg ccctggccga gcctcatgcg cgcgtactgg aacaacacgg
    12841 ccgcctacta cggcaagttc ctcaatggct ggtacgtgtc cggggatcgg gccagcgtgg
    12901 acgacgacgg gtacttctgg ttctgcgggc gcgacgacga tgtgatcaac acggccgggc
    12961 acctggtcgg gccgttcgaa gtggagagcg cgctgctgga gcacccggcc gtggccgagt
    13021 cggcggc
//
LOCUS       DAOSIF010000028        12960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_4880_length_12960_cov_4.973111, whole genome shotgun sequence.
ACCESSION   DAOSIF010000028 DAOSIF010000000
VERSION     DAOSIF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12960
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4880_length_12960_cov_4.973111"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1215)
                     /locus_tag="PLT35_01985"
     CDS             complement(34..1215)
                     /locus_tag="PLT35_01985"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOG28024.1"
                     /translation="MRRRPAALSQLVLPALAVLALAAAGASCWTGGAPDLGAPQRIAD
                     GVRLYRLHDPALIEPAGPIAVQVLRLDPSKVDLRSALPGGRVVQLDTVLGIARREGAV
                     AAVNAGFFVLKTGDPAGVLEVGGELVSDSPLTRGAVGIVREAGKPLSLVFDRLAATVT
                     LRYATDDGRVARRVDGVDTTRARGKLMLYTPRYGPDSDTAGTGVEWQLAGSPLRVVRR
                     ARAGKTPIPRGGAVLSYGGTDLPSDLEALEPGREVALVTEFRPLLGTTPEQWARAQDI
                     VGGAGLLVHRGKPLTDWSSEQLAPRFDADRHPRTMIGATRDGTIWMVTVDGRNPMLSL
                     GMSFPELQGLARRLGLHDALNLDGGGSTTMVAGGSVVNHPSDAQGPRRVSDALVVVPK
                     R"
     gene            complement(1248..3242)
                     /locus_tag="PLT35_01990"
     CDS             complement(1248..3242)
                     /locus_tag="PLT35_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28025.1"
                     /translation="MNGPDPPLRPGRGLAGPVAVLLLAVFAAVLVRTAWASDDALISL
                     RTVLNLTHGNGLTFNAGERVQTFTHPLWLALLTGAYLVLGNVYYAAFALSIGVSIAVF
                     WLAVSRAASRWQGWLAAAALLSSRAFVDFSTSGLENPLANLLVAALAAVFLREGGRAR
                     RRVAGLWALGSLLYLTRPDAVVIAAPLLAEATWRAWRDGERAGALAGAAALGLAPALA
                     WTAFAVVYYGFPFPNTAYAKLAADVDRWQMWKQGVIYAVDSLDRDPLTLVFIAFALAT
                     AASAGTRGARPLAAGIALYLAYVTSIGGDFMSGRFYALPFFASAILLTRMADAARPVW
                     IAAIAASAVLGAASAKAPILSDSRFEDRGAKHGGIVDERVFYLRKHSLVYASMLSFAD
                     PDWEVHRPPGDTPVLNTCGLMGEGGLDFGPHYYLLDECALADPLLARLPAIFREEWRP
                     GHFARAVPEGYRESVATGTNRIKDPRLREFYGHLSRITRSKALWSRDRLWTIWKMNTG
                     AYDRLVDRDFYRFGGFVKTLAELSDVKAPETPWDAPGTTVLTRPLAVRVDRKAGRRYV
                     DVSLDSDDKYVLVFVLSRAAVGRVEVGPIPQHRRKPGLASYTIDVPSRAEREGFDVVL
                     VVPIAGDERYAIGHLLVEGYPPTDAQLRERVAARDGVAPR"
     gene            complement(3239..4618)
                     /locus_tag="PLT35_01995"
     CDS             complement(3239..4618)
                     /locus_tag="PLT35_01995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG28026.1"
                     /translation="MTTLTFLGAAGTVTGSKYLLETGGRRLLVDCGLFQGLKELRERN
                     WEPLPLDPKALDAVILTHAHIDHSGYLPRLVKDGFKRRVFCTPATADLCRIMLPDSGR
                     LNEDDARDANRDGYTKHAPALPLFTENDAYEALTRLQPVGYERTMPLDGAASFSFADA
                     GHLLGSSFVRLSLADRGREILFSGDLGRYGRPVLPDPSTVPSADVLVVESTYGDRTHE
                     RDDGGAKIAEIVSSTIAAGGKVIIPAFAVGRVEEVIYWLKRLEEERRIPVVPVYLDSP
                     MAVDALRHYASHSRDLDPDVQSRRGEMGAFTTRRFTAVASPQQSKEIQTSPSPCVVVS
                     ASGMATGGRVLHHLKAALPRPRNTVLFVGYQAAGTRGRSLIEGAQTVKIHGQQVSVAA
                     RIERIDSMSAHADADEILRWLGGFRRPPETTYIVHGEPPARAALKARIERELGWRVHV
                     PEHLERVVV"
     gene            complement(4615..5598)
                     /locus_tag="PLT35_02000"
     CDS             complement(4615..5598)
                     /locus_tag="PLT35_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOG28027.1"
                     /translation="MTAFAGALDRLAAIPSLDLWAPPSPIHGLPRLGAALGGGPRLLV
                     KRDDAIPFGFGGNKVRKVAIVAAAAVAEGADTLLSVGGVQSNSARVVAAVAAQAGLRC
                     VLVANGRRPDPLTGNALLVSLLGAEVHYVETREERAPAMARIAEALRREGRHPYEIPL
                     GASTPLGALGFVRAVGEMAAQGVVPDVIVHATSSGGTQAGLVAGCALHGLPTRVLGIS
                     ADDPAPSVRGAVAGIIAGMGPLLGVDGEALALARPIDVDAAHVGGGYGVPSPASLEAA
                     DLFARLEALFADHTYTAKALAGLVHHIRCGAFTAGQTVLFWHTGGQAGLFA"
     gene            5632..6486
                     /locus_tag="PLT35_02005"
     CDS             5632..6486
                     /locus_tag="PLT35_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28028.1"
                     /translation="MTTSSILRALSTDARVVAAELRPPRAELGGSEGMDAWIDTYHAV
                     RRLTREGTCVCLTDSAVGIPEEDNLRHLTANLGPDAPRERVVPFLTSKHPFEYCLSYA
                     DRAWQHGFPALVVLGGDRRVGPPRSVEHAWQLRKAIRARQPDLVLGGWANPHADPAWQ
                     ADLVASADFTAEFFLTQIVSHHDAARVERFVREVDRRGAGRPGVFGVFYYRSARPATL
                     ALLREFLPVPAEELTREFEAGATPIDVCARTLRTLGDAGARHFYISNLPLERTSETLA
                     EVLGRAGI"
     gene            complement(6619..7359)
                     /locus_tag="PLT35_02010"
     CDS             complement(6619..7359)
                     /locus_tag="PLT35_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG28029.1"
                     /translation="MTLEHRVVLITGPRRIGAEVARRAAARGADVALSYHQSQREAEA
                     VAAAIRAAGRRAECIQADLADGHACRALIGETVARLGRLDVLVNMASLYVPTPFDALD
                     EAAWDRGLAVDVKAAFLCSQAAVPHMRRAGGGRIINVADWLPASGRVRYKGFLPYYVA
                     KSGVIALTEALALELASDDILVNAVAPGPILAPPGTEEEEVRAVEQATPLGRWGGEAE
                     VAKAVLFLIDTDFVTGEVVRVDGGRHLR"
     gene            7563..8912
                     /locus_tag="PLT35_02015"
     CDS             7563..8912
                     /locus_tag="PLT35_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOG28030.1"
                     /translation="MRTRELSPARLLSLHIAGLVLAGAVLLGLPVSSASGLALPPLDA
                     LFTSTSAVCVTGLTVLDTPKDLSLFGQVVLALLIQAGGLGYMTISSVVMLAFGRRMSM
                     HDRMALADSLNVSTMEGLLRFARTVAALTLAIEGAGALVLGARFALDAGWARGLWLGV
                     FHAVSAFNNAGFSLFSDNLTGYRGDVTVNLAVTALIICGGLGFFVLSGILRLRPRSAI
                     RMSLQTKLVLISSAVLVAGGTAAILALEWANPRTLGPLGAGEKVLAAYFQAVTPRTAG
                     FNTISIGDMTQPALFLTILLMFIGASPGGTGGGVKTTTFGVIVASIVSTVRGRRDTVI
                     FQRRVPAETIGRAFALTTIAFLGVVAVTGLLLLIERRDMLATMYETTSAFGTVGLSMG
                     APGSAVSLSGLFGPGGKLLVVLMMFMGRVGPLTLAVALAGSAPQEGVRYPEGRVSIG"
     gene            8912..9595
                     /locus_tag="PLT35_02020"
     CDS             8912..9595
                     /locus_tag="PLT35_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOG28031.1"
                     /translation="MKKMSIAVIGLGRFGSAMAVSLTELGQDVVGVDSDEDRVQKHAD
                     LISSAVALDATDERALKRAGIADVDAAVISIGENIEASLLAVMLLKDLEVPRIIAKAV
                     TPLHGRILQRLGVDRVIFPEREMAIRVAHSLVVANALDYIELSRDFSIVEVPAPAEFA
                     GRTLKDLQLRNRYGLTLIAIKRKSGAGSEETTIAPSADDLIESGDVLSLLGPNERLAH
                     IDRLLAARK"
     gene            complement(9615..9998)
                     /locus_tag="PLT35_02025"
     CDS             complement(9615..9998)
                     /locus_tag="PLT35_02025"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28032.1"
                     /translation="MAIRTELTIVLENRPGAAARVFASLADPRINIVAMQLEHGGALR
                     LVVDNPLHAERLLQERAMPYERRDVLYTMLPNDPGALARTAKLLADAGINVEYFYSTA
                     VEGQPMATVVIGVPDVLKASAAAGI"
     gene            10542..10865
                     /locus_tag="PLT35_02030"
     CDS             10542..10865
                     /locus_tag="PLT35_02030"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOG28033.1"
                     /translation="MRVEQASAGRSRFGDVVIVLFLLAQAADGVMTYVGVSTFGVSLE
                     ANPLLASLMAVFGQGATVAGAKLAAAFLGISLHLVGVHRVLASLTALYVLAALVPWIA
                     TLIML"
     gene            complement(10970..12217)
                     /gene="kbl"
                     /locus_tag="PLT35_02035"
     CDS             complement(10970..12217)
                     /gene="kbl"
                     /locus_tag="PLT35_02035"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR01822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOG28034.1"
                     /translation="MAYPAAVRDAWRAELAGIREKGIFKDERFIHSPQAARITVEYPA
                     GAPLREVVNLCANNYLGLSSHPAVVAAAHRALDERGYGMSSVRFICGTQDVHRALEEK
                     LTAFLGTEDTILFASCLDANAGVFEAILGPEDVMISDRLVHASIVDGIRLCKAMHDTY
                     KHCDMAHLEEKLREHQDKRVRVVITDGVFSMDGDMAPLDEIVALAEQYRALVFLDDSH
                     ATGFIGRTGRGVHERFGVMGKIDILTTTLGKALGGASGGCVSGRKEIVDLCRQRARPY
                     LFSNAVPPPIAASAIAVLDILSATTARRDALEANAAFWRAGLKDAGFVIKDGDSPIVP
                     VMLFNAKLAQEVARDLYDEGVYVVGFFFPVVAAGQARIRTQLSADHDIPMLERALEAF
                     RKVGAKHGILGLDKKGIIEKYGL"
     gene            complement(12220..12477)
                     /locus_tag="PLT35_02040"
     CDS             complement(12220..12477)
                     /locus_tag="PLT35_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG28035.1"
                     /translation="MKELILDYIKHEYLDADDAGDAPLTADTPLISSGIVDSFSMVSL
                     KRFLEKQYAIRIPDEEATPQAFDTVTSIVDLVARHLPKTGA"
     gene            complement(12612..>12960)
                     /locus_tag="PLT35_02045"
     CDS             complement(12612..>12960)
                     /locus_tag="PLT35_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28036.1"
                     /translation="VINTGGHLVGPFEIESAMLEHPAVAESAAVAKPDAVNMEVVKAF
                     VVLKPGHAPNDDLELEIMNTLRRRLSPLAMPQEIAFVDRLPKTGSGKILRRMLKAREL
                     GQSAGDLSTMDGG"
BASE COUNT         1797 a         4788 c         4525 g         1850 t
ORIGIN      
        1 cctgttcctc agccgatcat agccgactcc gggctacctc ttcggcacga ccaccagcgc
       61 gtcgctcacc ctcctgggcc cctgggcgtc ggacgggtgg ttcacgacgc tcccgccggc
      121 gaccatcgtc gtggatccgc cgccgtcgag attgagcgcg tcgtgcaggc cgagccgccg
      181 cgcgaggccc tggagttccg ggaagctcat gccgaggctc agcatggggt tgcggccgtc
      241 caccgtcacc atccagatcg ttccgtcgcg ggtggcgccg atcatggttc gcgggtggcg
      301 gtcggcgtcg aaccgcggcg ccagctgctc gctcgaccag tcggtcagcg gcttcccgcg
      361 gtgcaccagg agccccgccc cgcccacgat gtcctgcgcc cgcgcccatt gctccggcgt
      421 cgtgccgagc agcggacgga actcggtgac gagggcgacc tcccgccccg gctcgagcgc
      481 ctcgagatcg gaagggaggt ccgtgccgcc gtacgacagc acggccccac cgcggggaat
      541 gggcgtcttt ccggcgcggg ccctccgcac gacgcgcagc ggcgagccgg ccagctgcca
      601 ctcgacgccg gtgccggccg tgtcgctgtc tggcccgtag cggggcgtgt agagcatgag
      661 cttcccgcgg gcgcgcgtcg tgtccacgcc gtccacgcgc cgcgcgaccc ggccgtcgtc
      721 ggtggcgtag cgcagcgtca ccgtcgcggc gagcctgtcg aagacgagcg aaagcggctt
      781 gcccgcctcg cgcacgatcc cgacggcccc gcgggtcagc ggcgaatcgc tcaccagttc
      841 tccgccgact tcgaggacgc ccgccgggtc tcccgtcttc aggacgaaga accccgcgtt
      901 gacggcggcc accgcgcctt ctcggcgcgc aatgcccagc acggtgtcca gctggaccac
      961 gcgcccgccg ggcagcgccg atcgcagatc caccttcgac ggatcgaggc ggagcacctg
     1021 caccgcgatg ggcccggcgg gttcgatgag cgcggggtcg tgcagacggt agagccggac
     1081 gccgtcggcg atgcgctgcg gcgcgccaag atccggggcc ccgcccgtcc agcaggaggc
     1141 gccggccgct gccagcgcca ggacggccag agccggcagc acgagctggc tgagcgcggc
     1201 cggccggcgc ctcatcgttc tcaccggggc tcgccctgcc gcgcggccta ccgcggcgcc
     1261 acgccgtccc gcgcggcgac gcgctcgcgc agctgggcgt ccgtcggcgg atacccttca
     1321 acgagcaagt gcccgatggc gtagcgctcg tcccccgcga tcggcaccac gagcacgacg
     1381 tcgaagccct cgcgctcggc cctcgacggc acgtcgatcg tgtacgaggc gaggcccggc
     1441 ttgcgccggt gctgcgggat cgggccgacc tcgacccgcc cgacggccgc gcggctgagg
     1501 acgaagacga gcacgtactt gtcgtcggag tcgagagaga cgtccacgta ccggcgcccc
     1561 gccttccgat cgacgcgcac cgcgagcggc cgcgtcagca cagtcgtgcc gggtgcgtcc
     1621 cagggggtct ccggcgcctt gacgtcggac agctcggcca gcgtcttcac gaagcctccg
     1681 aagcggtaga aatcccggtc cacgagcctg tcgtacgcgc cggtgttcat cttccagatc
     1741 gtccacaggc ggtcccggct ccagagcgcc ttcgatcgcg tgatccgaga gaggtgccca
     1801 tagaactcgc gcagccgagg gtccttgatg cggttcgtgc ccgtcgcaac gctctcgcgg
     1861 tacccctcgg gcacggcccg cgcgaagtgc cccggcctcc actcctcgcg gaagatcgcc
     1921 gggagcctgg cgagcagcgg gtcggccagc gcgcactcgt cgagcaggta gtagtgcggt
     1981 ccgaagtcga gccccccttc tcccatgagg ccgcaggtgt tcaggacggg cgtgtcgcca
     2041 ggcggccggt ggacctccca gtccggatcg gcgaacgaca gcatgctcgc gtaaacgagg
     2101 gaatgcttcc tgaggtagaa cacgcgctcg tccacgatgc cgccgtgctt cgcgccccgg
     2161 tcctcgaagc ggctgtcgct cagaatcggc gctttcgccg acgcggcgcc gagcacggcc
     2221 gacgcggcaa tcgcggcaat ccagaccggc cgcgcggcgt cggccatccg ggtcagcagg
     2281 atcgccgacg cgaagaacgg cagggcgtag aaccggccgg acatgaagtc gccgccgatc
     2341 gacgtcacgt acgccaagta cagcgcgatg ccggccgcca gcgggcgggc gcccctcgtc
     2401 ccggccgatg cggccgtcgc cagggcgaac gcgatgaaga ccagcgtgag cggatcgcgg
     2461 tcgagcgagt caaccgcgta gatcacgccc tgcttccaca tctgccagcg gtcgacgtcg
     2521 gcggccagct tcgcgtaggc ggtgttcggg aacgggaacc cgtagtacac gaccgcgaag
     2581 gccgtccagg ccagcgccgg cgcgaggccg agagccgcgg cgcccgccag ggcgccggcg
     2641 cgctcgccgt cgcgccacgc gcgccaggtg gcctcagcga gcagcggcgc ggcgatgacg
     2701 acggcgtcgg gccgggtcag gtacagcagc gaccccaggg cccacagccc cgccacgcgc
     2761 cggcgcgcgc ggccgccttc gcgcaggaac acggccgcca gcgccgcgac gagcaggttc
     2821 gcgagcgggt tctccaggcc ggacgtcgag aagtccacga aggcgcgcga cgagagcagc
     2881 gcggccgcgg ccagccagcc ctgccaccgc gacgccgccc gcgaaacggc gagccagaag
     2941 accgcgatcg agacgccgat cgacagcgcg aaggcggcgt agtagacgtt gcccaggacg
     3001 aggtaggcgc ccgtgagcag ggcgagccac agggggtgcg tgaacgtctg gacccgttcg
     3061 cccgcattga aggtgaggcc gttgccgtgg gtgaggttca ggaccgtgcg cagcgagatc
     3121 agggcatcgt cgctcgccca tgcggtgcgg acgagcacgg cggcgaacac cgcgagcagc
     3181 agcaccgcga ccggcccggc caggccgcgc ccgggacgca gcggcggatc cggcccgttc
     3241 acacaaccac ccgctcgagg tgctccggca cgtgcacgcg ccagccgagc tccctctcga
     3301 tccgcgcctt gagcgcggcc cgagcgggcg gctccccgtg cacgatgtag gtcgtctccg
     3361 gggggcggcg gaagccgccc agccagcgca ggatctcatc ggcgtccgcg tgcgccgaca
     3421 tggagtcgat tcgctcgatc cgcgcggcca cgctcacctg ctggccgtgg atcttcaccg
     3481 tctgcgcccc ttcgatcagg ctgcggccgc gggtgccggc ggcctggtac cccacgaaca
     3541 gcaccgtgtt cctgggcctc ggcagcgccg ccttgagatg gtgcagcacg cgcccgcccg
     3601 tcgccatccc gctggccgac acgacgacgc agggcgaggg cgaggtctgg atctccttcg
     3661 actgctgcgg cgaggcgacg gccgtgaagc ggcgcgtcgt gaacgccccc atctcgccgc
     3721 gccgcgactg cacgtccgga tcgagatcgc ggctgtggct cgcgtagtgc cgcaaggcgt
     3781 ccaccgccat cgggctgtcg aggtacacgg gcaccaccgg gatgcggcgc tcctcctcca
     3841 gccgcttcag ccagtagatg acttcctcga cgcggccgac ggcgaacgcc gggatgatga
     3901 ccttcccgcc ggccgcgatg gtgctcgaca cgatctccgc gatcttcgcc cccccgtcgt
     3961 cacgttcgtg ggtgcggtcg ccgtaggtgg actccaccac gaggacgtcc gccgacggca
     4021 cggtggacgg gtcgggcagg accgggcggc cgtagcggcc gaggtcgccg ctgaacagga
     4081 tctcccgccc tcggtccgca agcgacaggc ggacgaacga ggacccgagc aggtgcccgg
     4141 cgtcggcgaa cgagaacgac gccgcgccgt cgagcggcat ggtgcgctcg tatccgacgg
     4201 gctgcaggcg cgtcagcgcc tcgtacgcgt cgttctccgt gaagagcggc agcgccggcg
     4261 cgtgcttcgt gtagccgtcc cggttcgcgt cacgcgcatc gtcctcgttc aggcgcccgg
     4321 agtccggcag catgatgcgg cacaggtcgg ccgtggccgg cgtgcagaag acgcggcgct
     4381 tgaacccgtc cttcacgagc cgcggcaggt agccgctgtg gtcgatgtgc gcgtgcgtga
     4441 ggattaccgc gtcgagggcc ttcggatcga gcggcagcgg ctcccagttg cgctcccgca
     4501 gctccttcag gccctggaag aggccgcagt ccacgagcag ccggcgcccg ccggtctcca
     4561 gcaggtactt ggacccggtg acggtgccgg cggcgccgag aaacgtcagg gtcgtcatgc
     4621 gaagagcccc gcctggccgc ccgtgtgcca gaagagcacc gtctgcccgg ccgtgaacgc
     4681 gccgcagcgg atgtggtgaa ccagaccggc cagcgccttc gcggtgtacg tgtggtccgc
     4741 gaagagggct tcgagccggg cgaagagatc cgcggcctcg agcgacgccg gcgagggcac
     4801 gccgtacccg ccgccgacgt gcgcggcgtc cacgtcgatc gggcgcgcga gggcgagcgc
     4861 ctcgccgtcc acgccgagca gcgggcccat gcccgcgatg atcccggcga cggcgccgcg
     4921 aaccgacggc gccgggtcgt ctgcgctgat cccgaggacg cgcgtcggca ggccgtgcag
     4981 cgcgcacccc gccacgagcc ccgcctgcgt accgcccgag gaggtcgcat ggacgatgac
     5041 gtcggggacg acgccctggg ccgccatctc gccgacggcg cgcacgaagc cgagggcgcc
     5101 gagcggcgtg gaggcgccga ggggaatctc gtacggatgc cggccctcgc ggcggagcgc
     5161 ttcggcaatc cgcgccatcg ccggggcccg ctcctcgcgc gtctcgacgt agtgcacctc
     5221 ggcgccgagc agcgagacca gcagcgcgtt gcccgtcagg ggatccggcc gccgcccgtt
     5281 ggcgacgagc acgcaccgca ggcccgcctg cgcggcgacg gcggcgacga cccgcgccga
     5341 gttcgactgc acgccgccga cgctgagcag ggtgtccgcg ccttccgcga cggcggccgc
     5401 cgccacgatg gcgaccttcc ggaccttgtt gccgccgaag ccgaacggaa tggcgtcgtc
     5461 gcgcttgacg aggaggcggg gaccgccgcc cagggccgcg ccaaggcgcg ggaggccgtg
     5521 gatgggcgag ggcggcgccc acaggtcgag cgacgggatc gcagccagac gatcgagagc
     5581 gccggcgaag gccgtcatgc ggggtattgt atactccggc cctgattcca cgtgacgacc
     5641 tcctccatcc tccgtgcgct ctccactgac gcccgtgtcg tcgccgcgga gctgagaccg
     5701 ccccgggccg agctcggcgg ctccgagggc atggacgcgt ggatcgacac ctaccacgcc
     5761 gtccgccgcc tcacgagaga gggcacctgc gtgtgcctga ccgacagcgc ggtcggcatc
     5821 cccgaagagg acaacctccg ccacctgacg gcgaacctcg ggcccgacgc gccgcgcgag
     5881 cgggtggtgc cgttcctgac gtccaagcac ccgttcgaat actgcctgtc gtacgcggac
     5941 cgcgcgtggc agcacggctt cccggccctc gtcgtcctcg gaggcgaccg gcgagtcggc
     6001 ccgccgcgct cggtggagca tgcgtggcag ctccggaagg ccatccgggc gcggcagccc
     6061 gatctcgtcc tgggcggatg ggcgaacccg cacgccgacc cggcctggca agccgatctc
     6121 gtcgcgtcgg cggacttcac cgccgagttc ttcctgaccc agatcgtctc gcaccacgac
     6181 gccgcgcgcg tcgagcgctt cgtgcgggaa gtcgaccgcc gcggagccgg ccggcccggc
     6241 gtgttcggcg tcttctacta ccggagcgcg agacccgcca cgctggcgct cctccgggag
     6301 ttcctgccgg ttcctgccga ggagctgacg cgggagttcg aggccggcgc gacgccgatc
     6361 gatgtctgcg cccggacgct gcgcaccctg ggcgatgccg gcgcgcggca cttctacatt
     6421 tcgaacctgc cgctcgagcg gacgtcggag acgctggcgg aggttctcgg gagggcgggg
     6481 atctagagga attcggaatc caggagtcag aagtcggaat ccgccgcgct gcagtccccg
     6541 accgccaagc cccagccgct cgagacccgg gtcccggacc ccgagtccca agtcccgacc
     6601 tgcggtcccg gccggcggct acctgaggtg cctgccgccg tcgacgcgca cgacctcgcc
     6661 ggtgacgaag tcggtgtcga tcaggaagag cacggccttg gcgacctcgg cctcgccgcc
     6721 ccagcgcccg agcggggtcg cctgctccac cgcccggacc tcctcctcct cggtgcccgg
     6781 cggcgcgaga atcggccccg gggcgacggc gttcaccagg atgtcgtcgc ttgccagctc
     6841 gagggccagg gcttcggtga gggcgatgac gccgctcttc gccacgtagt agggcaggaa
     6901 gcccttgtag cgcacgcgcc cgctcgcggg cagccagtcc gcgacgttga tgattcgccc
     6961 tccgccggcc cgccgcatgt gcgggacggc ggcctgcgag cagaggaacg cggccttcac
     7021 gtcgaccgcc agcccccggt cccacgcggc ttcgtccagc gcgtcgaacg gcgtgggcac
     7081 gtacagcgag gccatgttca cgaggacgtc gaggcggccc agcctcgcga ccgtctcgcc
     7141 aatcagcgcc cggcaggcgt gcccgtccgc caggtcggcc tggatgcact cggcccgccg
     7201 gcccgccgcc cggattgcgg cggcgacggc ttccgcctcg cgctgcgact ggtggtagct
     7261 gagcgccacg tcggcgcccc tggcggcggc gcgccttgcg acctcggccc cgatccgccg
     7321 cggcccggtg atgagcacca cccggtgttc gagggtcatg gagacattgt actcacccgt
     7381 gaacggagtc ttcggcgctc ggtgcggatc ggacgcggcg gtcgccgcgg tggcggcggc
     7441 ctcccggcct cccgccgtga acggccggcg tcaggcccct cgcccggccc gcgcctcatc
     7501 ccccgccccc gactcccgaa tcccgatccc gcggcccgaa tcccgcaata gaatgagaac
     7561 ccgtgcgcac gcgcgagctg tccccggcgc gactgctgtc gctccacatc gcaggcctcg
     7621 tccttgccgg cgccgtcttg ctgggccttc ccgtgtcatc cgcgtcggga ctggcgctgc
     7681 cgcccctgga tgcgctcttc acctccacct cggccgtgtg cgtgacgggg ctcaccgtcc
     7741 tcgacacgcc gaaggatctc tcgctcttcg ggcaggtcgt cctcgcgctg ctgattcaag
     7801 ccggcggcct gggctacatg acgatctcgt cggtcgtcat gctcgcgttc gggcggcgca
     7861 tgtccatgca cgaccggatg gcgctggccg actcgctcaa cgtcagcacc atggagggcc
     7921 tgctgcgctt cgcgcggacc gtcgcggcgc tcacgctcgc catcgagggc gccggcgcgc
     7981 tcgtcctcgg cgcgcgattc gcgctcgacg ccgggtgggc ccgcgggctg tggctcggcg
     8041 tcttccacgc ggtgtccgcg ttcaacaacg ccggcttctc cctgttcagc gacaacctga
     8101 cgggctaccg cggggacgtc accgtcaacc tggccgtcac ggcgctcatc atctgcggcg
     8161 ggctcgggtt cttcgtcctg tctggcatcc tcaggctccg cccgcggagc gccatccgca
     8221 tgtcgctgca gacgaagctc gtgctcatct cgagcgcggt cctcgtcgcg ggcggcaccg
     8281 ccgccatcct cgcgctcgag tgggcgaatc cgaggacgct gggccccctc ggcgccggtg
     8341 agaaggtgct ggctgcgtac ttccaggccg tgacgccgcg caccgccggg ttcaacacga
     8401 tcagcatcgg cgacatgacg cagcccgcgc tgttcctgac gatcctgctg atgttcatcg
     8461 gggcgtcccc tggcgggacg ggcgggggcg tgaagacgac gaccttcggc gtcatcgtcg
     8521 cgtcgattgt ctcgaccgtg cggggccggc gcgacacggt cattttccag cgccgcgtgc
     8581 cggccgagac catcggccgc gcattcgcgc tcacgaccat cgccttcctg ggcgtcgtgg
     8641 ccgtgacggg cctcctgctc ctcatcgagc gccgggacat gctggccacg atgtacgaga
     8701 cgacgtcggc gttcggaacc gtggggctct cgatgggggc cccgggcagc gcggtcagcc
     8761 tgtcgggcct gttcggccct ggcgggaagc tgctcgtcgt gctcatgatg ttcatgggcc
     8821 gcgtgggccc gctcacgctg gcagtggccc tggcgggctc ggccccgcaa gaaggcgtgc
     8881 gctatcccga aggaagggtg tcgatcggct gatgaagaag atgtctatcg ccgtcatcgg
     8941 cctcgggcgg ttcggctccg ccatggccgt ctcgctgacc gagctgggcc aggacgtcgt
     9001 cggcgtggac agcgacgagg accgcgtcca gaagcacgcc gatctgatca gctccgcggt
     9061 ggccctcgac gccacggacg agcgggccct gaagcgcgcg ggcatcgccg acgtggacgc
     9121 cgccgtcatc tcgatcgggg agaacatcga agccagcctg ctggcggtca tgctgctcaa
     9181 ggacctcgag gtcccccgca tcatcgccaa ggcggtcacg ccgctgcacg gccggatcct
     9241 ccagcggctc ggcgtcgacc gggtgatctt ccccgagcgc gagatggcga tccgcgtggc
     9301 ccacagcctc gtcgtggcga acgccctcga ctacatcgaa ctctcgcgcg acttcagcat
     9361 cgtcgaggtt ccagccccgg cggagttcgc gggccgcacg ctcaaggatc tgcagctgcg
     9421 caaccgctac ggcctcacgc tcatcgccat caagcgcaag agcggggccg gcagcgagga
     9481 aaccaccatc gcgccgagcg ccgacgacct gatcgagtcg ggtgacgtcc tgtcgctgct
     9541 ggggccgaac gagcggctgg cgcacatcga ccggctgctc gcggcgagga agtaggcgcg
     9601 cgccgcgggc gggctcagat gccggcggcc gccgacgcct tgaggacgtc gggcacgccg
     9661 atcaccacgg tggccatcgg ctggccctcg acggccgtcg agtagaagta ctcgacgttg
     9721 atgcccgcgt cggcgagcag cttcgccgtc cgggcgagcg ccccggggtc gttcggcagc
     9781 atcgtgtaga gcacgtcccg cctctcgtac ggcatggccc gctcctgcag cagccgctcg
     9841 gcgtgcaggg ggttgtccac caccaggcga agcgcgccgc cgtgctccag ctgcatggcg
     9901 acgatgttga tgcggggatc ggccaggctg gcgaagacgc gggccgccgc gcccgggcgg
     9961 ttctcgagga cgatcgtgag ctccgtgcga atcgccatga catgctcccg gaagcgttcc
    10021 cgaatccccc aggacgactc ggaagtatag cgtcaacccg cctgcacggg ccgccgggcg
    10081 gggctgccca cgacagggcg gcggggcttg acgacaggtt cacgacgggc ccgagcgagg
    10141 aaccaggaac cggacgcctc ggggcctccg ctcgaccctg ggccggcagc cgctccggaa
    10201 gctcggtcag ggcggccctt gcatcgccgg agcggagccg ccgcggcggc tcgccgcacg
    10261 ccgggctcgg cgcgcggaaa tcccgctacg ccgcgcgccc ctcgtcctgt tgcgcgcttc
    10321 tgcccgcccc ttcacgaaat gtgggaatgg cctgcgcatc ccggctggat ttcgggcaga
    10381 atctgcctgg gccggcgttc ctcgcacccg ctccggctgt gatcgcttct tgcagccgga
    10441 agggtatggc gtccgctcct cctcccgtca cgaggcccgg gtcagagccg atgggggccg
    10501 aggggcgcct gcgccggcgt cgcacagtct ggaggccacg cgtgcgcgtc gagcaggcgt
    10561 ccgccgggcg ctcgcgcttc ggcgacgtcg tcatcgtgct gttcctgctc gcccaggcgg
    10621 cggacggagt gatgacctac gtgggggtct cgaccttcgg cgtgtccctc gaggcgaacc
    10681 ccttgctcgc ctccctcatg gccgtcttcg ggcagggcgc cacggtcgcc ggcgccaagc
    10741 tggcggccgc cttcctcggc atctcgctcc acctcgtcgg cgtccaccgc gtgctcgcgt
    10801 ccctgacggc cctctacgtg ctggccgccc tcgtgccgtg gatcgcgacg ctgatcatgc
    10861 tctaggggct gggggctgag gcgagagagg cggactctca cgactaaccc gccccacgcg
    10921 gcgacagccg cgtcccacta gcgcctagcg cctagcgcct agcgcctgac tacagcccgt
    10981 acttctcgat gatccccttc ttgtccagcc cgaggatgcc gtgcttcgcc ccgactttcc
    11041 tgaaggcctc gagggcgcgt tccagcatgg ggatgtcgtg atccgccgag agctgcgtgc
    11101 ggatccgcgc ctgcccggcc gccacgacgg gaaaaaagaa gccgacgacg tagacgccct
    11161 cgtcgtagag gtcgcgcgcg acctcctgcg cgagcttcgc gttgaagagc atgaccggca
    11221 cgatcgggct gtcgccgtcc ttgatcacga acccggcgtc cttgaggccc gcgcgccaga
    11281 aggccgcgtt cgcctcgagc gcgtcgcgcc gcgcggtcgt cgcgctcagg atgtcgagca
    11341 ccgcgatggc cgacgccgcg atcggcggcg gcacggcgtt gctgaagagg tacggccgcg
    11401 cccgctgccg gcacagatcc acgatttcct tgcggcccga cacgcagccg ccgctcgcgc
    11461 cgccgagggc cttgccgagc gtcgtcgtga ggatgtcgat cttccccatc acgccgaagc
    11521 gctcgtgcac gccccgcccg gtgcggccga tgaacccggt cgcgtggctg tcgtcgagga
    11581 acaccagcgc ccggtactgc tcggccagcg ccacgatctc gtcgagcggc gccatgtcgc
    11641 cgtccatcga gaacacgccg tcggtgatga cgacccgcac ccgcttgtcc tggtgctccc
    11701 gcagcttctc ctccaggtgc gccatgtcgc agtgcttgta cgtgtcgtgc atcgccttgc
    11761 acaggcggat gccgtccaca atcgacgcgt gcaccaggcg gtccgagatc atcacgtcct
    11821 cgggcccgag gatcgcctcg aacacgcccg cgttcgcgtc caggcagctg gcgaagagga
    11881 tcgtgtcctc ggttcccaga aaggccgtca gcttctcctc gagcgcccgg tgcacgtcct
    11941 gcgtcccgca gatgaaccgc acgctcgaca tgccgtagcc gcgctcgtcg agagcccggt
    12001 gcgcggccgc cacaaccgcg gggtggctgg acagaccgag gtagttgttc gcgcagaggt
    12061 tgaccacctc tctcaggggc gccccggccg ggtactcgac cgtgatccgc gcagcctgcg
    12121 gcgaatggat gaagcgctcg tccttgaaga tgcccttctc gcggatgccg gccagctccg
    12181 cacgccaggc gtcccgcacg gccgcagggt acgccatggc tacgcccccg tcttcggcag
    12241 gtgcctggcg accaggtcga cgatgctggt cacggtgtcg aacgcctgcg gcgtggcttc
    12301 ttcgtccggg atgcggatcg cgtactgctt ctcgagaaac cgcttcaggc tgaccatgct
    12361 gaagctgtcg acaatgccgc tcgagatgag cggcgtgtcc gccgtcagcg gcgcgtcgcc
    12421 ggcatcgtcc gcgtcgaggt actcatgctt gatgtagtcg aggatcaatt ccttcatcgg
    12481 atgctccctg agtcaggggc ccggggccgg acgcgggccg ccggccggcc gggcccgtca
    12541 gcgtcgcccc ggccgcgccc gcgcggccag cgccgcctcc cgccaggccc tcgcgcccgg
    12601 ccacccggcg cctagccgcc gtccattgta ctgaggtcgc cagccgactg gccgagttcc
    12661 cgcgctttca gcatccggcg caggatcttg ccgctccccg ttttgggcag ccggtccacg
    12721 aaggcgatct cctgcggcat cgcgagcggc gagagccgcc gccgcagggt gttcatgatc
    12781 tccagctcca ggtcgtcgtt cggcgcgtgc cccggcttga ggacgacgaa cgccttgacg
    12841 acctccatgt tcacggcatc cggcttggcc accgccgccg actcggccac cgcggggtgc
    12901 tcgagcatcg ccgactcgat ctcgaacggc cccaccagat ggccgccggt gttgatcacg
//
LOCUS       DAOSIF010000029        12613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5034_length_12613_cov_4.467192, whole genome shotgun sequence.
ACCESSION   DAOSIF010000029 DAOSIF010000000
VERSION     DAOSIF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_12613_cov_4.467192"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLT35_02050"
     CDS             <1..945
                     /locus_tag="PLT35_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28037.1"
                     /translation="YFGDTKLREGRLRAAYDVIVFPHVGGTAQAMVNGIARTGSEPLP
                     YRKSELTPNLGAQDQADDIRGGMGIEGLMNLYQFVRDGGLLIVEGATSTIFPEYKLVN
                     GVTVESPPGLFVRGSIVRGMVADAKSPILYGYDGAQIPVYFNQDPVLAVGGFGLGGGR
                     GGAAGAQIPGVGMTVAPNAASTQKLSPWKLETRPEEERRADPAASAGGGRSAGTGPAG
                     TGQAAGRGAAAAFSGAGSEAPRVILRFPSNPDEMLLSGVLVGGQALAGRAQVVDVPIG
                     SGHVVMFAIRPYWRWQTQGTFCLGFNAILNWNDLDAGR"
     gene            1097..3175
                     /locus_tag="PLT35_02055"
     CDS             1097..3175
                     /locus_tag="PLT35_02055"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG28038.1"
                     /translation="MKRAVTAAVAIALASASLALLADEGMWTFDNVPRARIKQQYGFE
                     ITDGWLEHVRLASVRVGDGGSGSFVSPEGLLFTNQHVGRGQVAKLSLPDRDLVRDGFY
                     APSREAEIACPDLELNVVVSYEDVTARVQGAVTAGASDADANAQRKAEMAVIEKACAA
                     GTGLKCEVVTLYSGGEYWLYRNKKYTDVRLVFAPEEQIAYFGGDYDNFTYPRWNFDIA
                     FFRAYEGGAPAKTPQYLKWSKAGAAEGELIFTPGYPGSTARLLTVAQLRYHRDVGNPL
                     QLRVWRSRRDALVRYASTGAEQARRASAARLGLENSLKRLVGQQDGLLNPRIFGRKEA
                     DEADLRAKVAARAEWRRAFAPAWEEIEHAYAGLPATAARLAFSTLSASRLANMASTIV
                     RYGDEMKKPSGQRYPEYADARLEGLRFGLYSPAPVYADLEEAQLAAWLEEARDALGAE
                     DPFVKAALGSLTPAEAARQAVGGTALAAPAARKALVEGGPSAIEASADPLIALARRVE
                     PVIRELRAWQEERIQSVEARAGQKIAEARFAVYGKTAYPDANFNLRLEYGRVLGYEEG
                     TTLVPPHTTFYGLFERAAAFGGKPPFNVPARWRDGRAKLDLATPFNFVYTADTIGGNS
                     GSPVINRDAEICGINFDSNLQKLPNRYLYIDEAEGSRAVGVHSAGILEGLKKLYAADA
                     LVAELTSQRQ"
     gene            3289..3519
                     /locus_tag="PLT35_02060"
     CDS             3289..3519
                     /locus_tag="PLT35_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28039.1"
                     /translation="MSRNDEAAPRSVRGRQLACPVCRHTQFYSRSYLLNTRVASFFNF
                     DWANRSALTYVCEQCGHILWFAADPDHPDHQE"
     gene            complement(3644..4198)
                     /locus_tag="PLT35_02065"
     CDS             complement(3644..4198)
                     /locus_tag="PLT35_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28040.1"
                     /translation="MIAAASRLLPKRLYHRLREAGLSAIARRFRRRDPAFSASIPVPG
                     TTMVLRGLSRADFPVAAAYFQSGLSERDVRLALVPNEAAFNDPARLNYGVFDGPVMVA
                     AANLVIRGLGKAEGGLSVAASHRRRGLGSFLHEHFEGLCSRQGYVLLARIDARNSQAL
                     AFISTRPYREIAAAGAFRCFEKQP"
     gene            complement(4255..5313)
                     /locus_tag="PLT35_02070"
     CDS             complement(4255..5313)
                     /locus_tag="PLT35_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG28041.1"
                     /translation="MPRNDFGQLVGRSPAILALQEEIRRVALSDAKILITGESGVGKE
                     LVALSIHSHSLRADRPFVTVNCAGLPETLLESELFGHVKGSFTGAYRDKAGMLEKADE
                     GTIFLDEIGEMSLRMQGMLLRFLETGELQKVGADRVGRHVDVRVIAATNRLIADMVSQ
                     GSFREDLYYRINVINIQVPPLRERREDIPMLVEAFLESLDNDRKLRVTPEAYAALAEY
                     SWPGNVRELANVIERIVVSGCGPEIGLADVPVEVRSQRGISLRPKRERRRSVADELYK
                     RMTVHRESFWTAVYPLYMQREITRGNLRELVSKGLEEAKGNYKIVTKLFNMDPGDYKR
                     FLNFLRKHDCQLPFRDYR"
     gene            complement(5306..5647)
                     /locus_tag="PLT35_02075"
     CDS             complement(5306..5647)
                     /locus_tag="PLT35_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG28042.1"
                     /translation="MDRGLVERRRSPRVPLGGAAVMIRPVSMAVRVLDVSSDGVLLAC
                     PDPIAAEAGPRVIARLGDRALDARLDVRHVSRQWDQRSGGYRVGGRFLDLAPEARTAV
                     DCLLRGSDHGA"
     gene            complement(5649..6002)
                     /locus_tag="PLT35_02080"
     CDS             complement(5649..6002)
                     /locus_tag="PLT35_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28043.1"
                     /translation="MRLADRRAATRFEIVGELWGSVQALEPLHVFNLAPEGALVESVA
                     PLPVGSVQPIRVVRDGQAIDMRAIVRHLSPIHLQDGGRRYRVGLEFIDIGKRAAESIG
                     ALMDECREPPPANED"
     gene            6331..7605
                     /locus_tag="PLT35_02085"
     CDS             6331..7605
                     /locus_tag="PLT35_02085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG28044.1"
                     /translation="MRADPQPELTLLTAVSIMAAMSQPWTSRLLAAFRLVIGRCWIDP
                     LETLLEEMIGLPRTSFWVPVRRAAEFARDLGRLVYGGPRVSVRRWVGPDWTVVLVSQD
                     DAIWDNELRHVLFAVSPAEHSGHRLWLWQVPALVRADAAADRLVVLHLNRLLKWRFPD
                     LWCVRVPPSLRAIMDISAPLEAVRAGMTKRRRQELTGLAKLGLEGGISHDLAEFSRFY
                     QDMHAPFVTERYRDRIRMRPLEAQKRVFERRGKLLWVKRQGMMVAAVFGVRRRFGKTF
                     CAIHLGIDQSQDEAIRRHAITALYWRLITWSRDAGCRSIDFGPAPAQLGDGLFLYKHR
                     WGMRFDRDRTLHSVWTFAGRSLPSALVRRLNELGLVAEVARAYKCAIFPGEAGTGLSA
                     REEARRVKIMAQARLDGFLPVGATGAFVSPSR"
     gene            complement(7589..8572)
                     /locus_tag="PLT35_02090"
     CDS             complement(7589..8572)
                     /locus_tag="PLT35_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28045.1"
                     /translation="MVISKTPLRISFAGGGTDFRDYYRARQGRVVSMAIRRHVYVTVN
                     KRWDDAIRVAYTKTEIVRHIGDLQHDLIREALKMAGLTRSIEVTTIADVPAGSGLGSS
                     SSLTVGALNALYAFQGRYLPPGLLAEQACQIEIGVLGRPIGKQDQYIAAHGGFRFLHF
                     NADESVSVEPIVCTDATRRRLVNSLLLFYTGVSRESHSVLAEARQRMQVPGASRSALD
                     GLSDLAARVAREVAGGRLSRFGSFLHEGWELKKQMGSKVTNGDLDRYYRLARRAGAEG
                     GKITGAGGGGCLLLYAPRSAHASIRATARRLGFREFPIGFEPDGSKIIYSD"
     gene            complement(8581..9279)
                     /locus_tag="PLT35_02095"
     CDS             complement(8581..9279)
                     /locus_tag="PLT35_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG28046.1"
                     /translation="MKAFLMAGGLGERLRPITDRMPKCLVPIRGVPLLGIWLDLLARE
                     GVDEALVNVSRFPELVEAFLDSRAARHPVVRLVRERSPAGSAGTVAAHRAFVDGEESF
                     WIVYADNLTNASLSRLLAAHRANDGVATLALFRTPSPRSSGIVGIEPDGRIVRFREKP
                     DHPEGNLANAGIYLARQAIFDWIPAGQGVTDFGHDVFPRMVGRMYGYEVDGYLIDIGT
                     PERLEQANADWPGF"
     gene            complement(9276..10577)
                     /locus_tag="PLT35_02100"
     CDS             complement(9276..10577)
                     /locus_tag="PLT35_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG28047.1"
                     /translation="MPLTPAWIATALGLAITGAFSWLAARDMRRATLVFLVMGWVPFV
                     RVFTFHGSQIQQPLLLAEALPTVMIAIWWLRRARGPAPPLVPAPVNRPLLLMVPASAL
                     ALLWSFGDLDPAVPAANVKLAVSLGQILLIAWPVGLYFVVANTAWDAAAIRRVRTALT
                     IMAAPSVALPLVPVEWRSHITWSAYFALAAGPWLVAEAFEERALARRLGLWIVALSPL
                     LYGLVIGKAFLYLTTLVAVAAVVFIRARKTVGVGVLGAAGIYLLLVAATGSLTPGPLR
                     DLLDVERRQQSWGGQAGRLALAADAVEIWSRHPLLGVGPGNSWPYMHRYSTLDTPHNQ
                     YLNVLLEFGAVGLACFVWFLAAALKTGRDALRRARDGPARNLVIGWLALFIGMIASGL
                     TGDYIFHSVRNGGLLLFSAYYFQWVLLGLVVAASAGGGDGA"
     gene            10813..11490
                     /locus_tag="PLT35_02105"
     CDS             10813..11490
                     /locus_tag="PLT35_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG28048.1"
                     /translation="MPFVSSALLRENRIPIRGGVLPLDRRLNQNIKAISDRLLGAAAA
                     LLAAPLMLVVAALVKLTSEGPIIHRRRVVGRGGVEFDAFKFRTMVANADQILAADPAL
                     RREFLQKHKIEDDPRVTPVGRFLRKLSLDELPQFFNVLAGQMSVVGPRMLSPEEIDRY
                     GAHVAALLSVKPGLTGLWQVSGRQRTTYERRIQLDMQYVRTWSLWLDLCILARTPIEV
                     FRGTGAF"
     gene            11524..12177
                     /locus_tag="PLT35_02110"
     CDS             11524..12177
                     /locus_tag="PLT35_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOG28049.1"
                     /translation="MMWRDSRTYLAELMQALRLIDTRTIERFGRLLHRTYVLNRTVFC
                     IGNGGSAALASHLAADLAKLTTTPGQPRLKAMSLADSMAAVTAAANDIGYEDVFVEQL
                     RTYLEPGDVVIAFSTSGRSRNVLRALEYANGRGAVTIGITGADGEPLRAASAESLSIA
                     SLSVQQIEDASTVAAHLICLVTRELCLAEIRPDSTDLLDTPVPVEQLRLTLDTNPSA"
     gene            12331..>12613
                     /locus_tag="PLT35_02115"
     CDS             12331..>12613
                     /locus_tag="PLT35_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28050.1"
                     /translation="MPTPDVTVSLVSFNTRDLLRAALQAVEASRGVSCEVFVVDNGSA
                     DGSADMVEAEFPAARLIRNPDNRGFAAANNLAIRRAAGRYVLLLNPDTEV"
BASE COUNT         1995 a         4328 c         4420 g         1870 t
ORIGIN      
        1 tacttcgggg acacgaagct gcgggagggc aggctccggg cggcgtacga cgtgatcgtg
       61 tttccgcacg tcggcgggac cgcgcaggcg atggtgaacg ggatcgccag aacgggatcg
      121 gaaccgctgc cctaccggaa gtcggagttg acgccgaacc tcggcgcgca ggaccaggcc
      181 gacgacatcc gcggcgggat gggcatcgag ggcctgatga acctctacca gttcgtcagg
      241 gacggaggcc tcctgattgt cgagggggcg acctcgacga tcttccccga gtacaagctc
      301 gtcaacggcg tcacggtgga gagcccgccg ggcctgttcg tgcgcgggtc gatcgtccgc
      361 ggcatggtcg ccgacgcgaa gagcccgatc ctgtacggct acgacggcgc gcagatcccc
      421 gtgtacttca atcaggaccc cgtgctggct gtcggcggat tcggcctggg aggcgggcgc
      481 ggaggcgccg ctggcgcgca gatcccgggc gtcgggatga ctgtggcgcc caacgcggcc
      541 tcgacgcaga agctgtcgcc gtggaagctg gagacccggc cggaggagga gcgtcgagcc
      601 gacccggccg cctccgccgg cggcgggcga agcgccggga ccggcccggc gggaaccggc
      661 caggccgccg gacgcggcgc ggccgcggcg ttcagcggag ccgggtccga ggcgcctcgc
      721 gtgatcctgc gcttcccgtc gaacccggac gagatgctgc tctcgggcgt cctcgtcggc
      781 ggccaggccc tcgcgggccg cgctcaggtg gtggacgtgc ccatcggctc cgggcacgtc
      841 gtgatgttcg cgatccgccc ctactggcgg tggcagaccc agggcacgtt ctgcctcggc
      901 ttcaacgcga ttctcaactg gaatgacctg gacgcgggga ggtagaggag gaatccggaa
      961 ttcggaatga agatggagcc ccgggtcttc agagctctgg ggagttggtc cagggggcag
     1021 gggacgaacg tgacggggtg agcctttacg acgtgaattc ctgtgggatg gcggcgggac
     1081 cgccggaggt gcttgcatga agcgagccgt gactgccgcc gtcgcaatcg cgctcgcgtc
     1141 cgcgtccctg gccctgctgg ccgacgaggg gatgtggacg ttcgacaacg tgccccgggc
     1201 gcgcatcaag cagcaatacg gcttcgagat caccgacggg tggctcgagc acgtcaggct
     1261 ggcgtcggtg cgcgtcggcg acggcgggtc gggatcgttc gtgtcgccgg agggcctgct
     1321 cttcaccaac cagcacgtcg gccgcgggca ggtggcgaag ctgtctctcc cggatcgcga
     1381 cctcgtccgg gacggcttct acgcgccgtc gcgggaggcg gagatcgcct gcccggatct
     1441 cgagttgaac gtcgtcgtgt cgtacgagga cgtgacggcg cgcgtgcagg gcgccgtgac
     1501 ggccggcgca agcgacgcgg acgccaacgc ccagcgcaag gccgagatgg ccgtcatcga
     1561 gaaggcgtgc gcggccggga cgggcctcaa gtgcgaggtc gtcacgctct acagcggcgg
     1621 cgagtactgg ctctaccgca acaagaagta caccgatgtc cggctcgtct tcgcgccgga
     1681 ggagcagatc gcgtacttcg gcggggacta cgacaacttc acctacccgc gctggaactt
     1741 cgacatcgcg ttcttccgcg cgtacgaggg cggggcgccg gcgaagaccc cgcagtactt
     1801 gaagtggtcg aaagcgggcg cggccgaggg cgagctgatc ttcacgccgg gctacccggg
     1861 atcgaccgcc cgcctgctga cggtcgcgca gctgaggtac caccgagacg tcggcaatcc
     1921 gctccagctg cgcgtgtggc gatcccggcg cgacgcgctc gtgcggtacg cctcgactgg
     1981 cgccgagcag gcgcggcgcg cgtcggccgc caggctcggc ctcgagaatt cgctcaagcg
     2041 cctcgtcggg cagcaggacg gcctgctcaa tccccgcatc ttcggcagga aggaagccga
     2101 cgaggcggac ctgcgcgcga aggtggccgc cagggcggag tggcggcggg cgttcgcgcc
     2161 cgcgtgggag gagatcgagc atgcgtacgc gggcctgccg gccacggcgg cccgtctcgc
     2221 gttctccacg ctctcggcat cgcgcctcgc gaacatggcg tccacgatcg tccgctacgg
     2281 cgacgagatg aagaagccga gcgggcagcg ctatccggag tacgccgacg cgcgcctgga
     2341 agggctgagg ttcggcctct actcgccggc gccggtgtac gccgacctgg aggaggcgca
     2401 gctcgccgcg tggctcgagg aggcgcggga cgcgctcggc gccgaggatc ccttcgtgaa
     2461 agccgcgctc gggagcctga cgccggccga ggcggcgcgg caggccgtcg gcgggacggc
     2521 gctcgccgcg cccgcggcca ggaaggcgct cgtcgagggc gggccctcgg ccatcgaggc
     2581 ctcggccgac ccgctcatcg cgctggcgag gcgggtcgag ccggtcattc gcgagctgcg
     2641 cgcctggcag gaggagcgca tccagagcgt ggaggcgcgc gcgggccaga agatcgccga
     2701 ggcgcggttc gccgtctacg gcaagaccgc ctatcccgac gccaacttca acctccggct
     2761 ggagtacggg cgggtgctcg ggtacgagga aggcacgaca ctcgtgccgc cgcacacgac
     2821 gttctacggc ctgttcgagc gcgcggcggc cttcggcggg aagccgccgt tcaacgtgcc
     2881 cgcgcgctgg agggacgggc gcgcgaagct cgatctcgcc acgccattca acttcgtcta
     2941 caccgccgat accatcggcg gcaattcggg cagcccggtg atcaaccgcg atgccgagat
     3001 ttgcggcatc aacttcgaca gcaacctgca gaagctgccg aaccggtacc tctacatcga
     3061 cgaggcggaa ggctcgcgcg cggtgggcgt gcactcggcc ggcatcctcg aggggctgaa
     3121 gaagctgtac gccgccgacg cgctcgtggc ggagctgacg tcacagcgac agtaaggggc
     3181 ggcagcgccc cgatgcggcg gccacctgcc gcaggcgccc gccagcggtc ggcggctcgg
     3241 cggcacggcg aagcccggcg gcggcgcgag tgaccaggga ggtcgcccgt gagcaggaac
     3301 gacgaagcgg caccgcgatc ggtccggggc cggcagctgg cgtgccctgt ctgccggcac
     3361 acgcagttct actcgcgctc ctacttgctg aacacgaggg tcgcgagctt cttcaacttc
     3421 gactgggcga accgctccgc cctgacctac gtgtgcgagc agtgcggcca catcctgtgg
     3481 tttgccgcgg acccggacca cccggaccac caggagtagg accgcgtccc gcgcggcgct
     3541 cccggtgagg cggctcgacg cgcgccgggc ccggcgctcg ccaatcggcg gcgtccgagg
     3601 gccttcttcg aggcgcagca ggagccgcat cccggggcca gcgtcagggc tgcttctcga
     3661 aacaccggaa ggccccggcc gccgcgatct cccggtacgg gcgggtcgag atgaaggcga
     3721 gcgcctggga gtttcgcgcg tcaatccgcg caagcagcac gtagccctgc ctcgagcaaa
     3781 gcccttcgaa gtgctcgtgc aggaaggacc cgaggccccg acgccggtgg ctggcggcca
     3841 cgctgagccc gccttcggcc ttgcccaggc cccggatgac caggttcgcg gcagcgacca
     3901 tgaccgggcc gtcgaacacg ccgtagttga ggcgagcggg gtcgttgaag gccgcttcgt
     3961 tcgggaccag cgcgagacga acgtcgcgct cggacaggcc cgactggaag tacgccgcgg
     4021 caacggggaa gtcggcccgg gacagcccgc gcaggaccat ggtcgtgccc gggaccggaa
     4081 ttgacgccga gaaggcaggg tcgcgcctcc tgaaccggcg ggcaatcgcg ctgaggccgg
     4141 cctctctgag gcgatggtac aggcgcttcg gcaagaggcg gctggctgcg gcaatcatcg
     4201 gaggcttccc gggagtcacg agcgtcgatg ccccatcggc aggccgacgc gcgcctaccg
     4261 gtagtcccgg aacggcagct ggcagtcgtg cttgcgcagg aagttcagga agcgcttgta
     4321 gtcgcccgga tccatgttga agagcttcgt gacgatcttg tagttgccct tggcctcctc
     4381 gaggcctttc gacacgagct cgcggaggtt cccgcgcgtg atctcccgct gcatgtagag
     4441 ggggtagacc gccgtccaga aggactcgcg gtggaccgtc atgcgcttgt acagctcgtc
     4501 ggccaccgag cgccggcgct cgcgcttcgg acgcaggctg atgccgcgct gcgaccggac
     4561 ttcgacgggc acgtcggcca gcccgatctc ggggccgcag ccggagacga cgatgcgctc
     4621 gatgacgttg gcgagctcgc ggacgttgcc gggccacgag tactcggcga gcgcggcgta
     4681 cgcctcgggc gtgacccgca gcttccggtc gttgtccagg ctctcgagga aggcctcgac
     4741 gagcatcggg atgtcctcgc gccgctcgcg gagcggcggc acctggatgt tgatgacgtt
     4801 gatccggtag tagaggtcct cccggaacga gccctgggac accatgtccg cgatcaaccg
     4861 gttcgtggcc gcgatgacgc ggacatcgac gtggcgcccg acgcgatccg cgccgacctt
     4921 ctgcagctcg ccggtctcga ggaagcggag cagcatgccc tgcatgcgca ggctcatctc
     4981 gccgatctcg tccaggaaga tcgtgccttc gtcggctttc tcgagcatgc cggccttgtc
     5041 ccggtacgcg cccgtgaagc tccccttcac gtggccgaac agctccgatt ccaggagcgt
     5101 ctcgggcagt cccgcgcagt tcaccgtgac gaaggggcgg tcggcgcgca ggctgtgcga
     5161 atggatgctc agcgcgacca gctccttgcc gacgccgctc tcgcccgtga tcaggatctt
     5221 ggcgtcgctg agggcgacgc ggcggatctc ctcctggagc gcgaggatcg cggggctccg
     5281 gcccacgagc tgcccgaagt cgttcctagg caccgtggtc gctccccctc agcaggcagt
     5341 cgacggccgt tctcgcctcg ggcgcgaggt cgaggaaccg tcctccgacc cggtagccgc
     5401 cggagcgctg gtcccactgc ctggacacgt gccggacgtc cagcctggca tcgagcgcac
     5461 ggtcgccgag cctggcgatc acccgcgggc cggcctccgc cgcgatcgga tccgggcagg
     5521 ccaggaggac gccgtccgag ctcacgtcga gcaccctgac ggccatcgaa accggccgga
     5581 tcatgacggc ggcgcccccc agcggcacgc gcggcgaccg gcgccgctcg accaggccac
     5641 gatccatgtc agtcctcgtt cgccggtggc ggctcccggc actcgtccat gagggcgccg
     5701 atgctctcgg cggcccgctt gccgatgtcg atgaactcga ggccgaccct gtaccgccgg
     5761 ccgccatcct gcagatggat cggcgacagg tggcggacaa tcgcccgcat gtcgatcgcc
     5821 tggccgtctc gcacgaccct gatcggctgc accgacccca ccggcagggg cgcgaccgat
     5881 tcgacgagcg cgccttctgg ggcgaggttg aaaacgtgca ggggctcgag ggcctggacc
     5941 gagccccaca gctcgccgac gatctcgaag cgcgtggccg cccgccggtc agctagtcgc
     6001 atacctgctc ccgtcgcctg cgcatggcca ggccaggagt cggaggcgcg cgggcgcgcg
     6061 aacagccgcg gccgcaaaga cccccgcccg atccgaagca aggacgacgc caacgttagt
     6121 gtcaggcgtt ggctggctcg gccgacaggc gcacgcggaa atccagcctg aaaaccaccg
     6181 cgttccttgt cgagacccac gaagtcaccc gcggggtggt cagaagtctg acggcagcgt
     6241 gacaagattc tgccaagaag cgcctcttca gtcaaccgcg gccaggacct ccggcgcgtc
     6301 caatcgcctt ccgaatcctc gaatgccgac atgcgcgcgg atccccagcc cgaattgacg
     6361 ctcctgacgg cggtatcgat aatggcggcg atgtcgcagc cgtggacgag ccggcttctc
     6421 gcggcgttcc gcctcgtgat cggccggtgc tggatcgacc ctctggagac gctgctggag
     6481 gagatgatcg gtctgcctcg cacctcgttc tgggtcccgg tgcgacgggc ggccgagttt
     6541 gcccgggacc tgggccgcct ggtctacgga ggaccgcgcg tcagcgtgcg ccggtgggtc
     6601 ggtcctgact ggaccgtcgt cctggtcagc caggatgacg ccatctggga caacgaactc
     6661 cggcacgtgc tctttgccgt ttccccggcc gagcattccg gccatcgcct ctggctgtgg
     6721 caggtgccgg ccctcgttcg ggcagacgcg gcggcagatc gcctcgtcgt gctccacctc
     6781 aaccgactgc tcaagtggcg gttccccgat ctctggtgcg tgagggtccc tccgtcgttg
     6841 cgagccatca tggacatctc ggcgcccctg gaggccgtaa gggcgggtat gaccaaacgc
     6901 cgccggcagg aactgacggg gctcgccaag ctcggtctcg agggcggcat cagccacgat
     6961 ctggccgagt tcagccggtt ctaccaggac atgcatgccc cgttcgtgac cgagcggtac
     7021 cgcgaccgga tccggatgcg gccgcttgag gcccagaagc gggtcttcga gaggcgcggc
     7081 aagctcctat gggtgaaacg ccaggggatg atggtggctg cggtgtttgg cgtgcgccgc
     7141 agattcggga agaccttctg tgcaatccac ctgggcatcg accagtcaca agacgaggcg
     7201 atccggcgcc acgcgatcac ggccctctac tggcgcctca tcacctggtc gcgcgatgcg
     7261 ggatgtcgat ccatcgattt cgggcccgcg cccgcgcaac tgggcgacgg cttgttcctc
     7321 tacaagcacc gctggggcat gcggttcgac cgcgaccgca cgctccactc ggtgtggacg
     7381 ttcgccgggc ggagcctgcc ctcggcactg gttcgccggc tgaacgagct gggccttgtg
     7441 gccgaagtcg cccgtgcata caagtgcgcg atcttccccg gggaggcagg caccgggctg
     7501 tcggcgcggg aggaagcccg gcgcgtgaag atcatggctc aggcccggct ggatggattc
     7561 ctgccggtgg gcgccaccgg cgccttcgtc agtccgagta gatgatcttg ctcccgtccg
     7621 gctcgaagcc gatggggaac tcgcggaagc cgaggcgtct ggccgtcgcc cggatcgagg
     7681 catgtgccga tcgcggagcg tagagcaaca ggcagccgcc ccctcccgcc ccggtgatct
     7741 ttccgccctc ggcgccggcc cggcgcgcga gccggtagta tcgatcgagg tcgccgttcg
     7801 tgaccttcga ccccatctgt ttcttgagtt cccagccttc gtgcaggaaa gacccgaacc
     7861 gggagagccg gcctcccgcg acctcgcggg ccactcgggc cgccaggtcg ctcaggccgt
     7921 ccagggcgct cctggacgcg ccgggcacct gcatgcgctg cctggcctcg gcgagcacgc
     7981 tgtggctctc ccgggacacg ccggtgtaga agagcagcag gctgttcacc aggcgccgcc
     8041 tcgtcgcgtc cgtgcagacg attggctcga ccgacacgga ctcgtcggcg ttgaaatgca
     8101 ggaacctgaa cccgccgtgg gccgcgatgt actggtcctg cttgccaatc ggtctgccga
     8161 ggactccaat ctcgatctgg cacgcctgtt cggcgagcaa ccctggtggc aagtaccgcc
     8221 cctggaacgc gtacagcgcg ttgagggcgc ccaccgtcag gctcgacgat gagccaagcc
     8281 cgctgcccgc cggcacgtcc gcgatcgtcg tgacctcgat gcttcgggtc aaccccgcca
     8341 tcttcagggc ttcgcggatc aggtcgtgct gcaggtcccc gatgtggcgg acgatttccg
     8401 tcttcgtgta ggccacccgt atggcatcgt cccaccgttt gttgaccgtc acgtacacgt
     8461 gccgacggat ggccatcgac acgacgcggc cctgcctggc acggtagtag tcgcggaaat
     8521 cggtgcctcc gcccgcgaag ctgatccgaa gaggcgtctt cgaaatgacc atgggaccct
     8581 tcagaagccc ggccagtcgg cgttggcctg ctcgaggcgc tccggtgtgc cgatgtcaat
     8641 caggtagccg tcgacttcgt acccgtacat ccgcccgacc atgcgcggga acacgtcgtg
     8701 cccgaaatcc gtgaccccct ggccggccgg tatccagtcg aaaatcgcct gccgcgccag
     8761 gtagattccg gcgttggcga ggtttccctc cggatggtcg ggcttctcgc gaaagcgcac
     8821 gatgcgtccg tccggctcga tcccgacgat gcccgacgag cgcggcgacg gcgtcctgaa
     8881 cagcgccagc gtcgccacgc cgtcgttcgc gcggtgcgcg gccagcagcc ggctgagcga
     8941 cgcgttcgtc aggttgtccg cgtacacgat ccagaagctc tcctcgccgt ccacgaacgc
     9001 acggtgggcg gcgacagtgc ctgcgctgcc cgcgggcgac cgctcgcgca cgagccggac
     9061 gacagggtgc cgggccgccc gcgagtcgag gaaggcctcg acgagctccg ggaagcgcga
     9121 cacgttcacg agcgcctcgt ccacgccttc ccgggccagc aggtcgagcc agatcccaag
     9181 caggggtacc cctcgtatcg gcacgaggca cttcggcatg cggtcggtga ttgggcggag
     9241 gcgctcgccg agtccgccgg ccatcaggaa ggccttcatg cgccgtcccc gccgccggcc
     9301 gacgctgcga cgaccaggcc aaggagcacc cactggaaat agtaggccga gaagagcagc
     9361 agcccgccgt tgcgcacgct gtggaagatg tagtccccgg tcaggccgct tgcgatcatc
     9421 ccgatgaaga gcgccagcca gccgatgacg agattcctgg cgggcccgtc cctggcgcgc
     9481 ctgagcgcgt cccgccccgt cttcagcgcc gcggcgagga accagacgaa gcaggccagt
     9541 ccgaccgccc cgaactcgag caggacgttg aggtactggt tatgcggcgt gtcgagcgtc
     9601 gagtagcggt gcatgtacgg ccagctgttg ccggggccga ccccgagcag cgggtggcgg
     9661 gaccagatct cgaccgcgtc ggccgcgagc gcgagccttc ctgcctgtcc gccccagctc
     9721 tgctgccggc gttcgacgtc cagcagatcg cgcagcggcc cgggagtgag agacccggtg
     9781 gcggccacga gcaggagata gatccctgcc gcgcccagca cgccgacgcc gacggtcttt
     9841 cgggctcgga tgaacaccac cgccgccact gcgacgaggg tcgtgaggta caggaaggcc
     9901 ttgccgatga ccaggccgta cagcagcggc gaaagcgcga cgatccaaag cccgaggcgc
     9961 ctggccagcg ctcgctcctc gaacgcctct gcaaccagcc acgggccggc cgcgagcgcg
    10021 aagtaggcgc tccacgtgat gtgcgaacgc cattcgacgg gcaccagggg cagcgccacc
    10081 gagggcgcgg ccatgatcgt cagggccgtt cggacccgcc ggatcgcagc ggcatcccag
    10141 gcggtgttcg cgaccacgaa gtagaggcca acgggccagg cgatgagcag gatctggccc
    10201 agcgagacgg ccagcttgac gttggcggcg gggacggccg gatcgagatc tccgaagctc
    10261 cacagcagcg cgagcgccga cgcgggcacc atcaggagca gcgggcggtt gaccggggcg
    10321 ggcacgagcg gcggcgcagg cccgcgcgcg cggcgaagcc accagatcgc gatcatcacc
    10381 gtcggcaggg cttccgccag aagcagcggc tgctggatct gcgagccgtg gaacgtgaac
    10441 acgcgaacga acggcaccca gcccatgaca aggaagacca gggttgcgcg gcgcatgtcc
    10501 cgcgcagcca gccatgagaa cgctcccgtg atggcgaggc ccagagccgt cgcgatccag
    10561 gcgggcgtca gcggcacagg ccgcctcccg ggtgccggga gtgccccgac ccggccatgc
    10621 cgactgcggc cgcgcgacgg aacggcaaag gctgggctga ctttagtcgt cctcctggag
    10681 ggtttctcgg ctggctgcct tcgccgcagc agtctgtctt cgagcgggtc aacatactga
    10741 ttagaaacga gttgtacgac gaagacaagg ccgtatcaat cctggcccac accatgctta
    10801 tctccatgca atatgccatt cgtatcatcg gctctgcttc gcgaaaaccg tataccgatt
    10861 cgcggcggag tcctgcccct ggaccggcgg ctcaatcaga acatcaaggc catctccgac
    10921 cgcttgctgg gcgccgccgc cgcgctcctg gccgcgccac tgatgctcgt ggtcgcggcc
    10981 ctcgtgaagc tcacaagcga aggcccgatc atccaccggc ggcgcgtggt cggcaggggc
    11041 ggcgttgagt tcgacgcgtt caagttccgc acgatggtcg ccaacgccga ccagatcctc
    11101 gcggccgacc cggcgctcag gcgcgagttc ctgcagaaac acaagatcga ggacgatccc
    11161 agggtcactc cagtcggacg cttccttagg aaactgagcc ttgacgagct tccgcagttc
    11221 ttcaacgtgc tcgcagggca gatgtcggtc gtggggccgc gcatgctgtc acccgaggag
    11281 atcgaccgct acggcgccca cgtcgcggcg ctcctgtcgg tgaagcccgg cctgacaggg
    11341 ctctggcagg tgtcgggccg gcagcgcacg acgtacgagc gccgcatcca gctcgacatg
    11401 cagtatgtca gaacatggag cctgtggctc gacctgtgca tcctggccag gacgcccatc
    11461 gaggtcttca gaggcacagg agcgttctag ccgggcccgc cggctgtcgg gagaactcgt
    11521 cgcatgatgt ggagagactc caggacatat ctggctgagc tgatgcaggc gctccgcctg
    11581 atcgacacgc ggacgatcga gcgcttcgga cggctgctcc accgcacgta cgtcctcaac
    11641 cggacggtgt tctgcatcgg gaacggcggg agcgcggcgc tggcgtcgca cctggcagcc
    11701 gacctcgcca agctgaccac gacgccgggc cagccgcgcc tgaaggcgat gtcgctggcg
    11761 gacagcatgg cggccgtcac ggccgcggcc aacgacatcg ggtacgagga cgtgtttgtc
    11821 gagcagcttc gcacctacct cgagcccggc gacgtcgtca tcgcgttcag cacgagcggc
    11881 cggtcccgga acgtgctcag ggccctggag tacgcgaacg gcaggggcgc cgtgaccatc
    11941 ggcatcaccg gcgccgacgg cgagccgctg cgcgccgcgt cggccgagtc gctgagcatc
    12001 gcgtcgctga gcgtgcagca gatcgaggac gcgagcacgg tggccgccca tctgatctgc
    12061 ctggtgacgc gcgagctctg cctggccgag atccggccgg attccactga tctgctggac
    12121 acgccggtgc ccgtggagca gctccgcctg acgctcgaca cgaacccgtc ggcctgacgc
    12181 ccgattcccc tgcttcggac cgtattccgc ggctcgcggg gttccgatac atccgacgat
    12241 cggcccggcc cgcgcgccgc ctcgcccgcg ccggcccgtc gcgcccaggg gcaggtcctc
    12301 gaggcagctt gtcttcagtg aatgtgcgag atgcccacgc ccgatgtcac ggtgtcgctc
    12361 gtgtcgttca acacccgcga cctcctcagg gccgccctgc aggcggtgga ggcgagccgc
    12421 ggggtgtcct gcgaggtttt cgtcgtcgat aacggctcgg ccgacggaag cgccgacatg
    12481 gtcgaggcgg agttccccgc cgcccggctc atccggaacc ccgacaaccg gggcttcgcg
    12541 gcggcgaaca acctcgccat ccggcgggcg gcgggccggt acgtgctcct gctgaacccg
    12601 gacactgagg tga
//
LOCUS       DAOSIF010000030        12387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5134_length_12387_cov_4.480863, whole genome shotgun sequence.
ACCESSION   DAOSIF010000030 DAOSIF010000000
VERSION     DAOSIF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12387
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5134_length_12387_cov_4.480863"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PLT35_02120"
     CDS             <1..1316
                     /locus_tag="PLT35_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28051.1"
                     /translation="DLPWNPQRIEQRIGRCHRYGQKHDVVVINFLNERNEADRRVLEL
                     LTEKFSLFSGVFGASDEVLGSIESGVDFEKRIPAIYQECRTPEAIDAAFRALQEEMDE
                     QIRARLDDTRRVLFEHFDEDVHQRLRLRLADARAQLDRVGQRFWLLTRHVLADRARFD
                     EAALAFDLDRPPREEISRGRYHLISKLQPSAGGGAAGESSRFLYRLSHPLGEYVLDEG
                     KTVATPVAQIVFDVTHHPARLAVVEALRGKRGYLTLTRLAIDSYDREEYLLFSGFADD
                     GAPLDQETMEKLFLCGGRVSAAVSIPEPQAQRLAAESERHAKATVSRSLEQNSKHFQE
                     AREKLEKWADDMVLAAEKSLADTKEQIKALRRQARLAATLEELLQIQGKTARLERQQR
                     RQRQEIFKVEDEIGAKHDQLVDQLEKRLTQRTSTETLFTIRWGVA"
     gene            1384..2595
                     /locus_tag="PLT35_02125"
     CDS             1384..2595
                     /locus_tag="PLT35_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28052.1"
                     /translation="MSKKAPAYERPQAQVLRDRLVEPRRLIQFVGGPRQAGKTTIVQQ
                     ALAGLATPSLYASADEPAVRDTAWLAAQWERARDLLSGPHGRGAILAIDEVQKVTGWS
                     ETVKRLWDEDSRRRRPLRVVLLGSAPLLMQHGLTESLAGRFETIPVGHWPFAEMQGAF
                     GWDLETYLYFGGYPGAAHLVDDPPRWRSYVVQSMIETTIARDVLLMSRVEKPALLRRL
                     FELGCRHSGQILSFTKMLGQLQDAGNTVTLAHYLDLLGGAGMLAGLQKYAGTAVRQRG
                     SSPKLQVLNTALMTAQSGLSPEDARADREFRGRLVESAVGAHLANAAAGGACELFYWR
                     ERNREVDFVLRAGRRVVAIEVKSGRAPDAFPGLAAFAASFKPQRTLIVGGDGIPLEEF
                     LSRPVTDWLQP"
     gene            2606..5851
                     /locus_tag="PLT35_02130"
     CDS             2606..5851
                     /locus_tag="PLT35_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOG28053.1"
                     /translation="MSQKYEKLKALLKELFQLDQPDLDFGFYRVMHARAAEVTQFLEQ
                     DLLPQVKAAFAQYQPADKAAIEKELAKVIAGIEAAGMNPEDSPKVKELRAKLADDAVD
                     LDALEAEVYDHLFSFFRRYYSEGDFLSKRVYKPGVYAIPYEGEEVKLHWANADQYYIK
                     TSEYLRDYAFRLRPGDDKNPMRVHFRLVDAAEGQHGNIKEQAGRERRFKLAAAPFAAV
                     EDGELVIRFTYEPDPAKQKELNAAATAAILGLADALLVEWQKLLGETHVRADGTASDT
                     TRLRVHLDRYTARNTFDYFIHKDLGGFLRRELDFYIKNEVMHLDDVENESVPRVEQYL
                     SKIKVIRRIAGKIITFLAQLEDFQKKLWLKKKFVVETSWCIRLGVIPEEFYPEIAAND
                     AQREEWVRLHAIDELKPVDRSLLESGKPGYSVPLTVGFLKAHPTLMVDTRHFDAGFTA
                     RLLEAIGELDEQTDGVLFHSENFQALSVMQARYREQVKCIYIDPPYNTGSDGFAYKDS
                     YPYSSWLTLLDDRVALARTLLESRGVFLSSVDDIEHSALVAICESHFSKDGRIGNLVW
                     KGATDNNPTRIAVEHEYILCYAKSNSELEENWSTESSDTKELMLSVFDSIRKKTTSVS
                     EREALFSAFATENRETLGDLFRYRRVDARGPFAARRNMENPGKPGYQYDVLHPATKKV
                     CAKPYWGWRFPERTMRQFLDEDRIIFGKDENKIPELKVYLSDVRFPLRSVFHLDARKG
                     SNDLDRLFGARDIFKNPKPVEVLTHFLPFTSGPKSIPVDFFAGSGTTGHAVINLNRED
                     GGQRKFILVEQADCFDTVLVPRLKKVTFTPEWKDGQPKRLATAEEAERSPRIMKGVRL
                     ESYEDTLNNLELRRIEQQQGRLDLAEAKGADKFREEYMLRYLLDVEARGSQSLLNVAA
                     FTDPIAYELKVKRPGSDESREVNVDLLETFNWLIGLTVQHIAAPQTFSAETERDGEGR
                     LRLKGRLRPAPNGGWWFRTVTGTTPDGRRTLVIWRKRPGGETPEGVEQDNLILDEWFT
                     RQGYSSKDSEFDLIYVNGGNNLENLKAPDDSWKVRLIEEDFFRLMFDTEGV"
     gene            5851..9141
                     /locus_tag="PLT35_02135"
     CDS             5851..9141
                     /locus_tag="PLT35_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG28054.1"
                     /translation="MPRGRPRGSAAAAAARRSRRANARASAPFNRKLVLNQWLLGLFG
                     VERFDQLAEHLKDEALEGMDENNVHRFHHALCLHLPVEQRPELPDELLLEHDQAIVSV
                     TQRLNERRLTRGEAPIVWKYFQYLALLFTKIYLDRYFRDPRALLVALNKRIATLNEGL
                     DEPDRVAPFDEAAEAWPQLNKIAFWMATGSGKTLLMHAHILQYQDFLETHGRSRELNR
                     IILLTPNEGLSQQHLREFEKAGIGAEIFDKDGGRLFAGQAVEILEVTKLKEDMGEKTV
                     AVDAFEGNNLVLVDEGHRGASAGGEGAWMKFRNALCEKGFSFEYSATFGQAVKGSQAL
                     TDLYARSTLFDYSYRWFYGDGFGKDYQILNLEDDSNAEWMTSYLTACLLAFFQQQRLY
                     REREGAFRPFNLERPLWIFVGGSVTATLAARDASDIVEILRFLSRYVANRADSIEHIR
                     RVLHEGLVTATGKNLFAGRFAYLNSCGLSPAQIFDETLATLFNAPAGGALHVENLKGA
                     TGEVAVRVGDNDPFGVINVGDDARLVKLCEEEGLNVAEREFTGSLFHELGQPHSTINV
                     LIGSKKFTEGWSSWRVSTMGLMNVGRGEGAQIIQLFGRGVRLKGIGMSLKRSARTTLP
                     EGLERPKHIDTLETLHIFGIRADYMAQFRDFLEEEGLPTDDERFEFILPIIKNLGSRP
                     LKTIRLKKEINGVSTEFGDAFRKLGPIPTIQPPNPTREEATAWLQKNQVVLNWYPKIQ
                     AMKSAGVAGGDAEGAPNQARLTGRHIAFLDLDRLCFELERFKAERGWHNLNLTRSGIA
                     DLLADPTWYRLLIPESELAFDSFEKVRVWQEIALALLRKYVERYYTFRKREWELPHLE
                     YRDIAADDPNFPCVVRETSPEYGYRILIEESQHEIVAKLSELKAAIEAGELKPWEFRG
                     IKAIWFGQHLYQPLLFLDGGAVEISPAPLNKGERRFVEDLKTFHDANPGFFAGKELYL
                     LRNLSKGRGVGFFEAGNFHPDFIVWQLEGKRQRVAFVDPKGIRNVGLQDPKIGFHETV
                     KEIEQRLGDSDVELESFIVSNTPSHVMRKQWGIEKDAMAKRHIVFQDEDKDTYIGAVL
                     GAGSRAGA"
     gene            9885..11507
                     /locus_tag="PLT35_02140"
     CDS             9885..11507
                     /locus_tag="PLT35_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG28055.1"
                     /translation="MRISRRGFVGSAAAGLGAAAALGGRAVEAGPQQPPQKQPFDTPP
                     PAIPRDRISSTVPSDVVVVGAGIAGLIAALSAAEAGATVTLIEKAPVFSARGGDNTAI
                     NSRLHERAGIRVDEDRILHDLMKVQGARIHHGIVRLWMRHSARTMNWVLDMVEAEGLK
                     SYLVIPNRTDAETFVIDRWPDPTGMPPNWDPADEYTMEYPTCHRIGDRAANQRRWLSI
                     VEKRAIARGARIHYRTKAVRLIRDDRAGRVSAVIAQDAGGKHIEYTAAKGVVLSTGDY
                     SANPDMIERYFPGQGLRPGLIPTSTGEGHQMAMWVGAVMEKTPHAPLNDAVHALGTNA
                     FLLVNSRGQRFCNEDLDSEAIARQAEEQHGCWLVVDASWPDDLPRMGLGFDRVFEATP
                     AVRREFQEKVAKSAVLEAPTVEGLAAKMKVPADALKRTLARYNELVKGGRDLDFGKRA
                     DRLTAVDAPPFYAQWNPNPDRPMLIFGGLLTDERLQAVDAAGRPIDGLFLAGNTVGGR
                     FKQAYPLLCPGISHGMAATHGFLAGRYAMGLT"
     gene            complement(11650..12228)
                     /locus_tag="PLT35_02145"
     CDS             complement(11650..12228)
                     /locus_tag="PLT35_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOG28056.1"
                     /translation="MRRILFFAIAGLLVLSASFSALERGQDTRPEAWAVKVESPHLTN
                     LWKVDDTLYRSEQPDAAAFKHLRDLGIKSVLNLRDRHVDKALLDGLDLVEYDVQIRAK
                     AFTDAEIVRALRAIMAARKPVLVHCQHGSDRTGAVIAMYRIVVQGWTREQALAEMQNG
                     GYGFHAKYVNIPEYIERVDIGRIRAELKKPGA"
BASE COUNT         2284 a         4073 c         4069 g         1961 t
ORIGIN      
        1 acgacctgcc gtggaacccc cagcgcatcg agcagcgcat tggccgctgc caccggtacg
       61 gccagaagca cgacgtcgtc gtcatcaact tcctgaacga gcgcaacgag gcggatcggc
      121 gcgttctcga gctgttgacc gagaagttca gtctcttcag cggcgtgttc ggcgcctccg
      181 acgaagtgct cggcagcatc gagtctggcg tggacttcga gaagcgcatc cctgcgatct
      241 accaggagtg ccgcacgccg gaggccatcg acgccgcctt ccgggctctc caggaggaga
      301 tggacgagca gatccgtgcc cgactcgacg acacccggcg cgtccttttc gagcatttcg
      361 acgaggacgt gcatcagcgg ttgcgcctgc gccttgccga tgcgcgggcc cagctcgatc
      421 gggtgggcca gcgtttctgg ttgctgaccc gccatgtact cgctgatcgg gcgcggttcg
      481 acgaggctgc gctggccttc gatctcgacc gcccgccgcg ggaggagatt tcgcgcggtc
      541 gctaccatct catctccaag ttgcaaccca gcgcgggcgg cggggccgcc ggagagtcga
      601 gccgctttct ctaccggctc tcgcacccgc tgggcgaata cgttctcgac gaaggcaaga
      661 cagttgcaac gccggtcgcc cagatcgtct tcgatgtcac ccaccatccg gcgcggctcg
      721 ctgtcgtcga ggccctgcgc ggcaagcgcg gctaccttac gctcactcga ctggcgatcg
      781 attcctacga tcgggaggag tacctgctct tctcgggttt cgccgacgat ggagcgccac
      841 ttgaccagga gacgatggag aagctcttcc tgtgcggcgg ccgtgtgagc gctgccgtga
      901 gcatccccga accccaggcg cagcgtctgg ccgccgagtc cgagcgacac gcgaaggcaa
      961 ccgtgagccg gtcgctcgaa caaaacagca agcacttcca ggaggcgcgc gagaagctgg
     1021 agaagtgggc cgacgacatg gtccttgcgg ccgagaagtc gctggcggac acgaaggagc
     1081 agatcaaggc cctccgccgt caggcgcgcc ttgcggcgac gctcgaagag ctactccaga
     1141 tccaagggaa gaccgcccgg ctcgagcgcc agcaacgccg ccagcggcag gagatcttca
     1201 aggtcgagga cgagatcgga gcgaagcacg accagctggt ggaccagctc gagaagcggc
     1261 tgacgcagcg cacatccacc gaaaccctgt tcaccatccg gtggggggtg gcgtgaggcg
     1321 ctaggggggc gttaccgcgg gcttgcgctc tgctcgaacg tctgagtaaa attactcaca
     1381 ccaatgagca agaaagctcc cgcctacgag agaccccagg cccaggtgct gcgggaccgg
     1441 ctggtcgagc cgcggcgtct catccagttc gtgggcgggc cgcgccaggc gggcaagacc
     1501 acgatcgtcc agcaggcgct cgcgggtctg gcaacgcctt cgctctacgc ctcggccgac
     1561 gagcccgccg tgcgcgatac cgcgtggctc gcggcgcagt gggagcgcgc gcgcgacctg
     1621 ctgtccggtc cgcacggccg tggcgccatc ctcgccatcg acgaggtgca gaaggtgacc
     1681 gggtggtccg agacggtgaa gcggctgtgg gacgaagaca gccgccgccg tcggccgctg
     1741 cgagtggtgc tgctcggatc ggcgcctctc ctgatgcagc acggactcac cgagagcctc
     1801 gctggccgct tcgaaacaat ccccgtcggc cactggccgt tcgccgagat gcagggcgcc
     1861 ttcgggtggg atctcgagac ctatctatac ttcggcggct accccggcgc ggcgcacctg
     1921 gtggacgatc cgccgcgctg gcgcagctac gtcgtgcagt cgatgatcga gacgacaatc
     1981 gcacgcgacg tgctgctcat gtctcgtgtc gagaagcccg ccctgctgcg ccgcctcttc
     2041 gagctgggct gccggcactc cggacagatc ctctccttca ccaagatgct cggccagctc
     2101 caggatgccg gtaacacggt cactttggcg cactacctcg acctgctcgg cggcgcgggc
     2161 atgctcgcgg gcctgcagaa gtacgccggc acggccgtcc gccagcgggg ctcgagcccc
     2221 aagctccagg tgctgaacac ggcgctcatg accgcgcagt ccggcctgtc tcccgaggac
     2281 gcgcgggccg accgcgagtt ccgggggcgc ctcgtcgagt cggccgtcgg cgcgcacctg
     2341 gccaacgctg cggccggtgg cgcctgcgag ctgttctatt ggcgcgagcg caaccgtgag
     2401 gttgatttcg tgctccgcgc cggccgccgc gtggtcgcca tcgaagtgaa gagcggccgc
     2461 gcgccggacg ccttcccggg cctggccgcg ttcgccgcgt cgttcaagcc ccagcgcacc
     2521 ctgatcgtgg gcggcgacgg tatcccgctc gaagagttcc tgtcgcggcc ggtcaccgac
     2581 tggctgcaac cgtgaggcac gcttgatgag ccagaagtac gagaagctga aggccctgct
     2641 gaaggagctg ttccagctcg accagcccga cctcgatttc gggttctacc gcgtgatgca
     2701 cgcgcgcgcc gccgaggtca cgcagttcct ggagcaggac ctcctgccgc aggtgaaggc
     2761 agccttcgcg cagtaccagc cggccgacaa agcggcgatc gagaaggagc tggccaaggt
     2821 gatcgccggc atcgaagccg ctggcatgaa tcccgaggat tcgccgaaag tcaaggagct
     2881 gcgcgcgaag ctggccgatg acgcggtgga cctcgacgcg ctcgaggccg aggtctacga
     2941 ccatctcttc agcttcttcc gtcgctacta ctccgagggc gacttcctca gcaagcgcgt
     3001 ctacaagccc ggcgtgtacg cgatccccta cgagggcgaa gaggtcaagc tccactgggc
     3061 caacgcggac cagtactaca tcaagaccag cgagtacctg cgcgactacg ccttccgcct
     3121 gcgcccgggc gacgacaaga acccgatgcg cgtgcacttc cgtctggtgg acgcggccga
     3181 gggccagcac ggcaacatca aggagcaggc gggcagggag cgccgtttca agctcgccgc
     3241 ggcgcccttc gcggcggtgg aagacggcga gctggtgatc cgcttcacct acgaacccga
     3301 cccggccaag cagaaggagt tgaacgccgc tgcgacagcg gcgatcctcg gcctcgcgga
     3361 cgcattactc gtcgagtggc aaaagttgct cggcgaaacc catgtccgcg ccgacgggac
     3421 agcgtccgac accacgcgcc tgcgggtgca cctcgaccgc tacaccgcgc gcaacacctt
     3481 cgactacttc atccacaagg acctgggcgg attcctccgc cgcgagctcg atttctacat
     3541 caagaacgag gtgatgcatc tcgacgacgt cgaaaacgaa agcgtcccgc gcgtcgagca
     3601 gtacctctcc aagatcaaag tcatccgccg gatcgcaggc aagatcatca ccttcctggc
     3661 gcagctcgag gatttccaga agaagctgtg gctcaagaag aagttcgtgg tggagacgag
     3721 ctggtgcatc cggctcggcg tgattccgga agagttctat ccggagatcg cggccaacga
     3781 cgcgcagcgc gaggagtggg tgcggctgca cgcgattgat gaactgaagc cggtcgatcg
     3841 cagcttgctc gaatcgggca agccgggtta cagcgtgccg ctcacggtgg ggtttctgaa
     3901 ggcgcatccg acgctgatgg tggacacgcg gcacttcgat gccggcttca cagcgcggct
     3961 gctggaggcc atcggcgagc tggatgaaca gaccgacggg gtgctcttcc acagcgagaa
     4021 cttccaggcg ctgtcagtga tgcaggcgcg gtatcgagag caagtgaagt gtatctacat
     4081 tgatccgccc tacaacaccg gtagcgacgg gttcgcctac aaagacagtt acccgtactc
     4141 gtcatggctg acgctgctcg atgaccgcgt ggcactggcc cgcactttgc tcgagtcgcg
     4201 cggcgtgttt ctgtcaagcg tagatgacat agaacacagt gctctggttg cgatctgcga
     4261 gagtcacttt agcaaggacg gccgaatcgg taaccttgtt tggaagggcg cgacagacaa
     4321 caacccgaca cgcatcgctg tcgagcacga gtacattctg tgctacgcga agtctaattc
     4381 ggagttagag gagaattgga gcaccgagtc cagcgacacg aaggaactca tgctcagtgt
     4441 ttttgacagt atccggaaga agaccacgtc ggtttctgaa agagaggcgc tgttctcggc
     4501 gtttgccact gagaatcgcg agacgctcgg ggatctcttt cggtacaggc gcgtggatgc
     4561 ccgtggtcca tttgccgcgc gccgcaacat ggagaaccca ggcaaacccg ggtaccagta
     4621 cgacgtgtta cacccggcaa ccaagaaggt gtgcgccaag ccttactggg gttggcgctt
     4681 cccagagcgg acaatgcgac agttcttgga cgaggatcgg atcatattcg gcaaagacga
     4741 gaataagatt cccgagctca aggtctacct gagtgatgtg cggtttccgt tgcgcagcgt
     4801 ctttcacttg gacgcacgca agggttccaa tgatcttgat cgactgttcg gtgcacgcga
     4861 catattcaag aatccaaagc ctgtcgaggt tctcacgcac ttcttgccgt tcacaagcgg
     4921 gccgaagtcg attcctgtag acttcttcgc cggctccggc accactggcc acgccgttat
     4981 caacctcaac cgcgaagatg gcggccagcg caagttcatc ctcgtggaac aggccgattg
     5041 cttcgacact gtgctggtgc cgcgcctgaa gaaggtcacc ttcacgccgg agtggaagga
     5101 cgggcagccg aagcgccttg ccacggcgga ggaagctgag cgcagcccgc gcatcatgaa
     5161 gggggttcgt ctcgaatcgt acgaggacac gctcaacaac ctggagctgc gccgcataga
     5221 gcagcaacag gggcggcttg atctcgcaga agccaagggt gcggacaagt tcagagagga
     5281 gtacatgctc cgctacctgc tcgatgtcga ggcccgcggg agccagtcgc tgctgaacgt
     5341 ggcggcgttc accgacccca tcgcgtacga gctcaaggtc aagcgccccg gctcggacga
     5401 gtcgcgcgag gtgaacgtcg acctgctgga gaccttcaac tggctgatcg ggctcacggt
     5461 gcaacacatc gccgcgccgc agaccttcag cgcggagacc gagcgtgacg gcgaaggacg
     5521 gctgaggctg aaagggcggc tcaggccggc tcccaacggc ggctggtggt tccgcacggt
     5581 caccggcacc acccccgacg gccgcaggac cctcgtcatc tggcgcaagc ggcccggcgg
     5641 cgagactccg gagggagtcg aacaggacaa cctgatcctc gacgagtggt tcaccaggca
     5701 gggctattcg agcaaggaca gcgagtttga cctgatctac gtgaacggcg gcaacaacct
     5761 cgagaacctg aaggctcccg acgacagctg gaaggtgcgc ctcatcgagg aagacttctt
     5821 ccgcctgatg ttcgacacgg agggggtctg atgccgcgcg gacgccctcg cggaagtgct
     5881 gcagcagcgg ccgcccgccg gagccgccgg gccaacgcgc gcgcttcggc gccgttcaac
     5941 cgcaagctgg tcctcaacca gtggctgctc ggtctgttcg gcgtcgaacg cttcgaccag
     6001 cttgctgagc acctgaagga cgaggcgctc gaagggatgg acgagaacaa cgtccaccgc
     6061 ttccaccacg cgctctgcct gcacctgccc gtcgagcagc ggcccgagct gccggacgag
     6121 ctgctgctgg agcacgacca ggctatcgtc tccgtcaccc agcgcctgaa cgagcggcgc
     6181 ctgacacggg gcgaagcgcc gatcgtctgg aaatacttcc agtatctggc gctcctcttc
     6241 accaagatct acctcgaccg ctactttcgc gacccgcgcg ccctgctggt cgcgctcaac
     6301 aagcggatcg cgacgctcaa cgagggtctg gacgagcccg accgggtcgc tcccttcgac
     6361 gaagccgccg aggcctggcc gcagctcaac aagatcgcct tctggatggc gaccggcagc
     6421 ggcaaaacgc tgctgatgca cgcgcatatc ctccagtacc aggacttcct cgagacccac
     6481 ggccgctcgc gtgagctgaa ccggatcatc ctgctcacgc ccaacgaggg actctcacag
     6541 cagcacctgc gcgagttcga gaaggccggt atcggggccg agatcttcga caaagacgga
     6601 ggacgcctct ttgccgggca ggcggtggag atcctcgagg tcaccaagct caaggaggac
     6661 atgggcgaga agacggtggc cgtggacgcc tttgaaggaa acaacctggt gttggtggac
     6721 gagggacacc gcggcgcgtc ggcgggcggc gagggcgcct ggatgaagtt ccgcaacgcg
     6781 ctttgtgaga agggcttctc cttcgagtac tccgccactt tcggccaggc cgtgaaggga
     6841 agccaggctc tcaccgacct ctacgcgcgc agcacgctct tcgactactc gtaccgctgg
     6901 ttttacggcg acgggttcgg gaaggactac cagatcctga acctggaaga cgacagcaac
     6961 gcggagtgga tgacgagcta cctcaccgcg tgcctgctcg ccttcttcca gcagcaacgc
     7021 ctctaccgcg agcgcgaagg ggcgttccgg cccttcaacc tggagcggcc gctgtggatc
     7081 ttcgtgggcg ggagcgtcac cgccacgctg gccgcgcgcg atgcctcgga catcgtggag
     7141 atcctgcggt tcctgagtcg ctacgtggcg aaccgcgcgg acagcatcga gcacatccga
     7201 cgggtgctcc acgaagggct ggtgacggcg accggcaaga acctgttcgc cgggcggttc
     7261 gcctacttga acagctgcgg gttgtcgccg gcacaaatct tcgacgagac gctggcgaca
     7321 ctgttcaacg cgcctgccgg tggcgcgctg cacgtcgaga atctgaaggg agcgacaggc
     7381 gaagtcgcgg ttcgcgtggg tgacaacgac cccttcggcg tcatcaacgt cggcgacgat
     7441 gccaggctcg tgaagctctg cgaggaagag ggcctgaacg tggccgaacg cgagttcacg
     7501 gggtcgctgt tccacgagct gggccagccg cattcgacca tcaacgtgct catcggctcg
     7561 aagaagttca cggagggctg gagcagctgg cgcgtgagca cgatggggct gatgaacgtg
     7621 ggcaggggcg aaggcgcgca gatcatccag ctcttcggcc gcggtgtgcg gctcaagggt
     7681 atcgggatga gcctgaagcg gagcgcccgc accacgctcc ccgaaggctt ggagcggccg
     7741 aagcacatcg acacgctcga aacgctccac atcttcggca tccgcgccga ctacatggcg
     7801 cagttccgcg atttcctcga ggaggaaggc ctgcccaccg atgacgagcg cttcgagttt
     7861 atcctgccga tcattaagaa cctcggctcg cggccgctga agaccatccg cctcaagaag
     7921 gagatcaacg gcgtcagcac cgagttcggc gatgccttcc gcaagcttgg ccccattccc
     7981 acgatccagc ctccgaaccc cacacgcgag gaagcgacgg cctggctgca gaagaaccag
     8041 gtggtgctca actggtatcc gaagatccag gcgatgaagt ccgcgggagt ggcggggggc
     8101 gacgccgagg gcgccccgaa ccaggcccgg cttaccggcc gccacatcgc gttcctggac
     8161 ctcgaccggc tctgcttcga gctggagcgg ttcaaggcgg agcgcggctg gcacaacctg
     8221 aacctcacgc gctcaggcat cgccgatctg ctcgcggacc cgacgtggta ccggctgctc
     8281 atcccggaat ccgagttggc gttcgactcg ttcgagaagg tgcgcgtttg gcaggagatc
     8341 gcgctggcgc tgctgcgaaa gtacgtcgag cgctattaca ccttccgcaa gcgcgaatgg
     8401 gagctgccac acctggagta ccgcgacatc gctgcggacg atcccaactt cccgtgtgtc
     8461 gtccgcgaga ccagccccga gtacggttac cgtatcctaa tcgaggagtc gcagcacgag
     8521 atcgtcgcga agctgagcga gctgaaggct gcgatcgaag ccggcgagct gaagccctgg
     8581 gagttccgtg gcatcaaggc catctggttc gggcagcacc tctaccagcc tttgctcttc
     8641 ctcgacggtg gcgcggtcga gatctctccc gcgcccctca acaagggcga gcgccggttc
     8701 gtggaggacc tgaagacatt ccacgacgcg aaccccggct tcttcgcagg gaaggagctg
     8761 tacctgctgc gcaacctgag caaggggcgc ggcgtggggt tcttcgaggc cggcaacttc
     8821 catcccgact tcatcgtctg gcagctcgag ggcaagcgcc agcgggtcgc cttcgtcgac
     8881 cccaagggca tccggaacgt gggcctgcag gatccgaaga tcggcttcca tgagacagtc
     8941 aaggagatcg agcagcggct cggcgattcc gatgtcgagc ttgagtcctt catcgtctcg
     9001 aacacgccat cgcacgtgat gcggaagcag tggggcatcg agaaagacgc gatggcaaag
     9061 cggcacatcg tcttccagga cgaggacaag gacacgtaca tcggtgccgt gctgggagcg
     9121 ggctcaagag ccggtgcatg atgtcggtcc gtcgtgcaga caaggcgtct gacaaccgac
     9181 tgcagcggac ggttcggcgc tctcggccgg gggcgaaggt cgggccaggg cggtaggttc
     9241 aatgcgggcc ggtctcagtg acgcgccggc accgccgctg aaacgcgagt cgttggccgg
     9301 accaagcgtc cgggtgtgga gtgagcgacg ctcgctgatt gctgcgagcc cgttgccaca
     9361 acatgtccac gacagcggtg gaatccaggg gacggaggaa cacgggcgtg aacggccgcg
     9421 tcccggcaat tgattgaacc cgaggtgctt gtggccagaa gtcgcgcgcc atcagccatg
     9481 caagagcacc gcgttccagg gttcgaaccc ctctctctcc gccagccctt gtttcccgag
     9541 agtcctcact gaccgggcct tgcaagaaac ggctccgacc gtgtccctcg tcccacccca
     9601 caggcggacg gcacctcagg ccgtcaagcg cgcggccgga agcctcgcgc ggagcaaccg
     9661 ctcgcgcgcc tgctggaggc cgcgccgcaa gagcctgatt gcaggcgggg cctgctccgc
     9721 cccgatgaac cgaaccttca ccgattcgac ttgacgaaga aacccgccgc tggttgtagg
     9781 atctcgtgcg gccgaaccga aggggtgaac cggatggggg acggacggac accgtccgca
     9841 aagcgacgat ccacgcaaac ggccaaccgg tatgaggtgc catcatgaga atcagcagaa
     9901 gagggttcgt cggcagcgcg gcagccggcc tcggcgcggc ggccgcgctc ggtggacggg
     9961 cggtggaggc cggcccacag cagccgccgc agaagcagcc gttcgacacg ccgccgccgg
    10021 ccatcccgcg cgaccggatc tcgagcaccg tcccatcgga cgtcgtcgtc gtgggcgccg
    10081 gcatcgcggg cctcatcgcc gcgctctcgg cggccgaagc cggcgcgacg gtcaccctca
    10141 tcgaaaaggc cccggtcttc agcgcgcgcg gcggcgacaa caccgccatc aacagccggc
    10201 tccacgaaag ggcgggcatc cgcgtggacg aggatcggat cctccacgac ctgatgaagg
    10261 tgcagggcgc gcggatccat cacgggatcg tccggctgtg gatgcgccac agcgcgcgga
    10321 cgatgaactg ggtgctcgac atggtggagg ccgagggcct gaagagctac ctcgtcatcc
    10381 ccaaccgcac cgatgctgag acgttcgtca tcgaccgctg gccggacccg accggcatgc
    10441 cgcccaactg ggatccggcc gacgagtaca cgatggagta cccgacctgc caccggatcg
    10501 gcgatcgggc cgcgaaccag cggcgatggc tctcgattgt cgagaagcgc gcgatcgccc
    10561 gaggcgcgag gattcactac cggacgaagg ccgtgcgcct gatccgggac gaccgcgcgg
    10621 gccgggtgag cgccgtcatc gcccaggacg cgggcgggaa gcacatcgag tacacggccg
    10681 cgaagggcgt cgtcctctcg accggcgact acagcgccaa cccggacatg atcgagcggt
    10741 acttcccggg gcaggggctg cgccccgggc tcatcccgac ctccaccggc gaaggccacc
    10801 agatggcgat gtgggtgggc gccgtcatgg agaagacgcc gcacgcgccg ctgaacgacg
    10861 cggtccacgc gctgggcacc aatgcgttcc tgctggtgaa ctcgaggggc cagcggttct
    10921 gcaacgagga cctcgattcg gaggccatcg cccgccaggc cgaggagcag cacggctgct
    10981 ggctcgtcgt cgacgcctcc tggcctgacg acctgccgcg gatgggcctg ggattcgacc
    11041 gggtgttcga ggccaccccg gccgtccgcc gcgagttcca ggagaaagtg gccaagagcg
    11101 ccgtgctcga ggcgcccacg gtcgagggcc tggccgcgaa gatgaaggtg ccggccgacg
    11161 ccttgaagcg cacgctcgcg cgctacaacg aactcgtcaa aggcggccgc gatctcgact
    11221 tcggcaaacg ggccgaccgg ctgacggcgg tcgatgcgcc gccgttctac gcgcagtgga
    11281 atccgaaccc cgacaggccg atgctcatct tcggcggcct gctgaccgac gagcgcctcc
    11341 aggccgtgga tgccgccggc cgtccgatcg acggcctctt cctcgcgggg aacacggtcg
    11401 gcgggcgctt caagcaggcc tatccgctgc tctgccctgg gatcagccac ggcatggcag
    11461 ccacgcacgg gttcctcgcc ggccgatacg cgatgggcct gacgtaggca gccgccggtc
    11521 ccggcgcttc gccgggatcc gggacatcga taaccgcgcc gggcggccga ctgccgccct
    11581 gcgcggcgta gatcgtggcg ggcatcggac tcgccttccg gatcgcgccg gtgcgggcac
    11641 gcgcccgcgc tacgcgcccg gcttcttcag ctccgcgcgg atgcggccga tgtcgacacg
    11701 ctcgatgtac tcggggatgt tgacgtactt cgcgtgaaac ccgtagccgc cgttctgcat
    11761 ctcggccagc gcctgctccc tggtccagcc ctggacgaca atcctgtaca tcgcgatgac
    11821 ggccccggtg cggtcgcttc cgtgttgaca atggacgagc accggcttgc gcgcggccat
    11881 gatggcccgc agcgccctga cgatctccgc gtccgtgaac gccttggccc ggatctgaac
    11941 gtcgtactcg acgaggtcca gcccgtcgag cagcgccttg tccacgtggc ggtcgcgcag
    12001 gttcagcacg ctcttgatcc cgagatccct caggtgcttg aatgccgccg cgtcaggctg
    12061 ctcgctcctg tacagcgtgt catcgacttt ccagaggttc gtcaggtgcg ggctctcgac
    12121 cttgactgcc caagcctccg gccgagtgtc ctggccccgc tcgagggcgg agaaagaagc
    12181 cgacagcacc agcaggccgg cgatcgcgaa gaacaggatc cttctcattg tcctcacctc
    12241 cgcgacgcca ccgctgaccg ccgtcagccg cggatgtcga cagtcgggcc ggcaccatcc
    12301 gctcgggatc gctccccgga tcgctccgca tacaggccgc cgcccggctc gcgttcgaac
    12361 gccgcaagga gcatagtgcg tcaggtg
//
LOCUS       DAOSIF010000031        12140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5271_length_12140_cov_3.577741, whole genome shotgun sequence.
ACCESSION   DAOSIF010000031 DAOSIF010000000
VERSION     DAOSIF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12140
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5271_length_12140_cov_3.577741"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="PLT35_02150"
     CDS             complement(<1..1615)
                     /locus_tag="PLT35_02150"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOG28057.1"
                     /translation="MKHPAIAALVLAWGAVTITAPAPHMPVAEIRPGMVGVGKTAFQG
                     TAIEEFQAHILGVLHSNVGPRRDLVLARLEGGPLANTGVIAGMSGSPVYVGGRLIGAV
                     SYSLGAFATEALAGITPIGEIVEAARSGLAGPAAAPPPVPVGSDLATLMSVVERALER
                     ARDQAGAPWALPPSGRGLPGAASLQPIALPLTLGGLGKQAAEAVSGPLRAAGFLPPAQ
                     APGGLPAASGAPLQPGEPIGVALITGDLQLGATGTVTEVDGQRVYAFGHAMYNLGPAC
                     FAMTRAYVHAVLPSLSQSMKIASVGETIGTIQQDRATTVAGLTGVRPDMVGMTVSLRR
                     DGGPARRFSFELGDHPLLTPLLAYTALTGIIVEHEHDIGHATYRVRARIAIDGHAPVE
                     IDDVHAGEQPGAAAASAVAVPLSSLLTNGRDPVRVRAISVDIDSAERLRTATIDRIWL
                     AAPEPRRGQTVPLKILLTPWRGEPAVRTVEIEIPRHAEGPLTLVVADGVSFAQWEQRE
                     RRAALAPSTLDQLIKALNQARRGNRVYVRL"
     gene            1791..2426
                     /locus_tag="PLT35_02155"
     CDS             1791..2426
                     /locus_tag="PLT35_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOG28058.1"
                     /translation="MSEGRRRLSVLDSRGGPGDAPAFARVAIAGLGTVGASLALALRR
                     AWPSTLVIGCDRNDRLEAAVSRGVIEVGADDLVIAGGADFVVLAGLPDRNAAALARLA
                     DLIQGRAVVMDLGGDEGTLGAAAATLPERLALISGALVPPLLEEGLEAASWRLFEGRA
                     WRLAGLRADARAWDCVERAVRATGGIPVRGQPGRPADPGSGGSSLGGRLSS"
     gene            2431..3054
                     /locus_tag="PLT35_02160"
     CDS             2431..3054
                     /locus_tag="PLT35_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG28059.1"
                     /translation="MVRADPELVRLALAGSQDASRQLVERHARAVYNLVARVVRDDGV
                     AEELTQEAFLKAFRALGSFDPAYKFSTWVLRIAHNLAIDHLRRVRPILVPIDDWSGSE
                     NPGDVLVDARAPSPLDHALRRDLRADLEAALSRLRPAFRSLVVMRYLEDLSYEEIALA
                     TGLPLGTVKSHLHRARAALARLMAESGWGPEPARTPGPVQPDPGSGA"
     gene            3071..3580
                     /locus_tag="PLT35_02165"
     CDS             3071..3580
                     /locus_tag="PLT35_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28060.1"
                     /translation="MPMEPDGRVTEWLEAEQAGRPDEADWRFRAVARGIGRLEPPAGL
                     AETVATRLAALRTKPDVWSTWWLRAAVASAILSAGAAGAALPPESWCRALLASLHAVA
                     LSLGGAAAAAWAWVAGVLAVWSGLGRAASILGRQFAGPELAALLAAQLALAAGAFAAL
                     HRLMREQEP"
     gene            3582..4964
                     /locus_tag="PLT35_02170"
     CDS             3582..4964
                     /locus_tag="PLT35_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28061.1"
                     /translation="MRSPVVAVALAVMASGLALTSVPASAEREGTRPAEAAPPDLRAL
                     RQRAEERFRIVEIRGGMILIPRSDAAGVRTIDVNDGRVLLDGTAVTGRELRDRLGRDA
                     DLVAQLSFLDAEGRRAFFAAATAEIPSPESEAPGAPGQPALPDVSGWPKAARGRHGGA
                     RVRIGGDVIVGRDEAVSDVVVVLGSARVDGLVESSVVAVGGSVVLGPTARVRGDVTSI
                     GGGVECDPGAVLEGESNEIAFSGPHLGPLLRVRPRRDWRWVIAPFDGRPRGTVDLLTT
                     LVRLGLAALFAAIVAAAAPGAVRGVSDRAAAEPWRAGLVGLAAELLFVPLLVLTVAVL
                     AISIIGIPLLLLVPFGLIAVGLAFVLGLAGAGRALGTCVARRVGGAAPGMPASLVLGL
                     AVIWALTVVARFVGLLGPPAGFFVAVLLVAGFLVEYVAWTVGLGAVILSRFGRRRSAP
                     AGPGLSDAVA"
     gene            5060..6595
                     /locus_tag="PLT35_02175"
     CDS             5060..6595
                     /locus_tag="PLT35_02175"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOG28062.1"
                     /translation="MRYTEQSLPVGVIASAWSSLDVDLLVVPVFEGEVPALPADLEAV
                     VGPLVRAVIERREFTPKPFATLVLPAAGPGWRPERVMLAGFGTRAECDARRVRRAASA
                     AALAARERRVRRLAMAASGALPLADEARAAAEGLTLASHYSGIYKSGDEARPAPIEAL
                     VVVPPATASADVREAEREAGRGYILASCSNLARALTNEPANVMTPRLFADIAGQVGSD
                     AGLRVDVLDEDRIAGLGMGLLLGVSRGSAEPPRVVVLRYDPPDAPARPLLGLVGKGVT
                     FDTGGISIKPSDRMHLMKTDMAGGAAVLSAMKAVAALKPNAPVIGVVPLVENMPGGRA
                     TRPGDVLRSASGKTVEVLDTDAEGRLILGDALWYARELGATHLLDAATLTGAIGIALG
                     RDMTGLFGTPAQWTDLVARTAARAGDRVWQMPLVDEYREQLRSEIADISNIGGRPAGS
                     ITAALFLQEFSGGRPWAHLDIASTAWADEAQPWQPKGPTGVAVRTMAELACTPDAWPP
                     PVA"
     gene            complement(6622..6870)
                     /locus_tag="PLT35_02180"
     CDS             complement(6622..6870)
                     /locus_tag="PLT35_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28063.1"
                     /translation="MLAAQPVTPVIVKVVPEPTPQVGAADVILDSLGLVGVIVIAALA
                     VGGLLGAVLIGIKRWREARHGGALPSDHTPLDLSSPNR"
     gene            complement(6881..8569)
                     /locus_tag="PLT35_02185"
     CDS             complement(6881..8569)
                     /locus_tag="PLT35_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG28064.1"
                     /translation="MFQAAAAREEALRQALAASPPGPAPTRDAIRQVVAAYRQVVLQY
                     PRSGYCDDALWLGAQLLAEAFSRYGNDQDRASAVTLLTSLAREYPSSSLAARARAEAA
                     RLRGLAAPAAPAADAGQAAPAPPGAAAASSAPRGRVLVRDIRRLVTPGGASVEVELDG
                     PVQFREQRLDQPPRLFVDLANTHTMDALRDASFAFESGAIKRLRVGRHPNDVTRIVIE
                     TGAATRCSSRLASAPYRVIISCDEPAVQTGVPAVGSVAEPAAADRPPEPARAPVQPAD
                     PGPRGAAPAPPPTPAPAVRPESPAAPSANGRGGYSMARQLGLRVGRIVIDAGHGGRDP
                     GAAGPGFNEADLTLDVALRLEKLLSGEPGIEVVLTRRTDEFVELHERTAIANRAGADL
                     FVSIHANASRNRNARGVECYLLNFASTPEAAAVAARENASSSLTMSHLNDLVKQIALN
                     SKLDESRDLAEHLQRSLVRRLRASNQGIRDLGVKQAPFVVLVGASMPSVLVEVSFLTH
                     RQEGRLLGTSSYRQRIAEAILDGIRGYLGTLKRVHTAAGAGGGPVRGQALEGLQ"
     gene            complement(8713..11064)
                     /locus_tag="PLT35_02190"
     CDS             complement(8713..11064)
                     /locus_tag="PLT35_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG28065.1"
                     /translation="MSASSTLSRRLSEFLGVAGFAVGLLWLIALATYSPADPAWFFNT
                     GSSAPAANLAGRVGAFVAELSHQVVGYAAWLFPLLVGAVGWRQFWCRPIEARYTKAIG
                     ALLLVGSASSFLSLALAPARTTGASFEPGGWLGDQFAGALVASFNRTGSLIVILACLG
                     LSLVLTTQFSFGRAFSAIASWTRERAATRVAAFWTWRDARRRERQRREIVSKHARRAG
                     TTDAARGSRPPGAEAGPNGRRAQPASAAVADPNAASAHRNGSALASSWPRQRKDTPIT
                     VVGPPAPAPPAADPPARSPAERKRNGYAFPPSTLLDAPRTERKIDERELMESARLLEE
                     KCREFAVAGSVVQIHPGPVVTTFEFKPEAGVKYSKVTGLADDLSLAMKAESVLIERTP
                     GKSTVGIQIPNQFRETISLREMLESAAYQASPSKLTLALGKTIQGEPFVADLATMPHL
                     LIAGATGTGKSVALNGMITSILFRATPEDVRMIFIDPKRLELGMYEEIPHLLTPVVVD
                     PKQAANALTWAVREMEERYKTLAAEGVRNIDQYNRNVRQAVLERSSSADEPLPKPLPY
                     IIVVIDELADLMMVARGEVEESVARLAQMARAIGIHLLLATQRPSVDVITGLIKANLP
                     ARISFRVSSKVDSRTVIDGNGAEQLLGRGDMLFSPPDSSRLVRVHGPYISEQETARLA
                     SFLRKQGKPTYDTSIIADEKAADAIEFEKDDLYDEAARLVVSTGQASISYIQRRLRVG
                     FSRAARLIDMMEAEGIVSPAMGGKPREVLVDKGYFDEVDQQLR"
     gene            complement(11068..>12140)
                     /locus_tag="PLT35_02195"
     CDS             complement(11068..>12140)
                     /locus_tag="PLT35_02195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOG28066.1"
                     /translation="GVTGSELSVIPAFEEIFASTRGKLVVTAFATSVYRLQILVDLAS
                     RFGRKVAFIGRGMVETSQLAQRLGYLSVPPGLAVRDADVKALPPSQVLCLVTGSQGEP
                     MSALSRIAIDDHRHVRLGPGDVVVFSAREIPGRERQIDRVVNHLARRGAEIVNQQTKH
                     VHVSGHGSEEELKLLLSLVRPRCFLPIHGTFRQRARHARVGERLARSVPGSMDVLVAE
                     NGDVLHFDAGGCRVAGRVPSGRVLIDGAGVTGLGDEILRDRRHLAEDGLIVPVLAINK
                     QTGAVEGVPEVITRGIVVPQGDEQPFRDLSRLIADIVDSASVEERTDYGIMRDKLGVE
                     LRRFFRKRVGQRPLVLPVIMEI"
BASE COUNT         1617 a         4240 c         4560 g         1723 t
ORIGIN      
        1 cgagacgcac gtacacccgg ttcccgcgcc gcgcctggtt tagcgccttg atgagctggt
       61 cgagcgtcga cggcgcgagc gcggcgcggc gttcgcgctg ctcccactgg gcgaagctga
      121 cgccgtcggc gaccaccagc gtcagggggc cctccgcgtg gcgggggatc tcgatctcca
      181 cggtcctgac ggccggttcg cccctccacg gggtgagcag gatcttcaac gggaccgtct
      241 gcccgcgccg cggctccggc gcagccagcc agatgcgatc gatcgtggcg gtccgaagcc
      301 gttcggcgct gtcgatgtcc acgctgatcg ccctgacgcg aaccggatcg cggccgttgg
      361 tcaacagcga gctgagcggc acggcgacgg cagaagcagc cgccgcgccg ggctgctccc
      421 cggcatggac gtcgtcgatt tcgacggggg catgcccgtc aatggcgatg cgggcgcgga
      481 cccggtacgt cgcatgtccg atgtcgtgct cgtgttcgac gatgatgccg gtgagcgccg
      541 tgtaggccaa gaggggagtc agcagcgggt ggtcgccaag ctcgaaggag aagcgccgag
      601 ccggcccgcc gtcccggcgc agcgacaccg tcatgccgac catgtccggg cgcacccctg
      661 taaggccggc gaccgtggtg gcgcggtcct gctgaatcgt gccgatcgtc tcgccaaccg
      721 acgcgatctt catcgactgc gagaggctcg gcaagacggc gtgcacgtag gctcgcgtca
      781 tggcgaagca ggcggggccg aggttgtaca tcgcgtggcc gaaggcgtag acgcgctggc
      841 cgtcgacctc cgtgaccgtg ccggtcgcgc cgagctgcag gtcgccggta atgagggcca
      901 cgccgatggg ctcgcccggc tggagcggcg cgccagacgc cgccggcagg ccgccgggcg
      961 cctgggccgg cggcaggaat cccgcggcgc gcaagggacc cgacaccgcc tcggcggcct
     1021 gcttgccaag cccgccgagc gtcagcggga gcgcgatggg ctgcagcgac gcggcgccgg
     1081 gcagcccgcg tccggacggt ggcagggccc atggcgcacc ggcctggtcc ctggcccgtt
     1141 ccaacgcgcg ctcgacgacg gacatcaggg tcgccagatc agagccgacc gggactggcg
     1201 gcggagctgc cgcggggccg gccagcccgc tgcgcgcggc ctccacgatc tcgccaatcg
     1261 gcgtgatgcc ggcgagggcc tcggtggcga acgcgccgag cgagtacgac accgcgccaa
     1321 tcaaccgccc gccgacatag acggggctgc cgctcatccc ggcaatcacg ccggtgttcg
     1381 cgagcggccc tccttcgagg cgcgccagga ccaggtctcg cctgggaccc acgttcgagt
     1441 ggagcacgcc caggatgtgc gcctggaact cctcgatggc cgtgccctgg aatgcggtct
     1501 ttcccacacc gaccatgccg ggacgaatct cggcaacggg catgtggggc gccggcgccg
     1561 tgatcgtgac ggcgccccac gccaggacga gcgcggcaat cgctgggtgc ttcacctgct
     1621 caccttatag cgagtatggc gccacagggt caaggacgcc caagcgcacg gacttcggca
     1681 gctcatgagg gaacggagca ggacaggatc acgtctcgac atgaaagcgc gcgccggcgc
     1741 gggcgcggcg cgagccgggg cagccgggcg gcgctggcgc tggaggggac atgagcgaag
     1801 gcaggcggcg gctctccgta ctcgactcgc gcggcggccc aggcgatgcg ccggccttcg
     1861 ctcgcgtggc gatcgcgggc ctgggtaccg ttggcgcttc gcttgcgctc gcgctccgcc
     1921 gcgcctggcc ctcgacgctc gtgatcggct gcgaccgaaa cgaccggctc gaggccgccg
     1981 tcagccgcgg agtcattgag gtcggtgccg atgacctggt gatcgccggc ggggcggatt
     2041 tcgtcgtcct cgccggtctt cccgatcgga atgcggccgc cctggctcgg ctcgcggatc
     2101 tcatccaggg ccgggccgtc gtcatggatc ttgggggaga cgaagggacg ctcggggcgg
     2161 cggcggccac gctgcccgag cgactggcgc tcatctcggg cgccctggtt ccgccgctcc
     2221 tcgaagaggg cctcgaggcg gcgtcctggc gcctcttcga agggcgcgcc tggcgcctgg
     2281 ccgggctgcg cgctgacgct cgcgcctggg actgcgtcga acgggctgtt cgggcgaccg
     2341 gcgggatccc cgttcgtggg caaccgggca ggcctgccga tcccggttcc ggcggatcgt
     2401 cgctgggtgg ccggctctcg tcttgaggcc atggtgcgcg cagacccgga gctcgtcagg
     2461 ctggcgctcg ccggctcaca ggacgcgtcc aggcagctgg tggagcgcca cgcgcgggcg
     2521 gtgtacaacc tcgtggccag ggtcgtccgc gatgacggcg tcgccgagga gctgacgcag
     2581 gaggcgtttc tcaaggcgtt ccgcgcgctc ggctctttcg accccgccta caagttctcg
     2641 acctgggtcc tccgcatcgc gcacaacctc gccatcgacc acctgcgcag ggtgcggccg
     2701 atcctggtgc ccattgacga ctggtcgggc tcggagaacc cgggcgacgt cctggtggat
     2761 gcgcgcgcgc catctcccct cgatcacgcc ctccggcgcg acttgcgcgc cgatctcgag
     2821 gccgccctct cgcggctgcg tccggcgttc cgatcgctcg tcgtcatgcg ctacctcgaa
     2881 gacctgtcgt acgaggagat cgcgctggcc acaggcctgc cgctcggcac ggtgaagagc
     2941 cacctccacc gggcgcgagc ggccctcgcg cgcctgatgg cagagtccgg gtgggggccc
     3001 gagcccgccc ggacgccagg cccggtgcaa cccgacccgg ggtcgggcgc gtagaaggga
     3061 ctggaaggtc atgccgatgg agccggacgg ccgggtgacg gagtggctgg aggccgagca
     3121 ggcgggacgg ccggacgagg cggactggcg cttcagggcc gtggcccgcg gaatcggccg
     3181 gctcgagccg ccggccgggc tggcggagac tgtcgcgacc aggctcgccg ccctgaggac
     3241 gaagccggat gtctggtcga cgtggtggct gcgggcggcc gtcgcgtcgg cgatcctgtc
     3301 ggccggagcg gctggagccg cactcccgcc ggaaagctgg tgccgcgcgc ttctggcgtc
     3361 gctgcacgcc gtcgccttga gcctgggcgg cgcggccgcg gcggcttggg cctgggtggc
     3421 cggcgtactg gcggtgtggt ccggcctcgg ccgggccgcc agcatcctgg gccgccagtt
     3481 cgccggcccg gagcttgcgg ccttgctggc cgcgcaactc gcgctcgcgg ctggcgcgtt
     3541 cgcggcgctg caccgcctga tgcgcgaaca ggagccctga tatgcgaagt cccgtggtcg
     3601 ccgttgcgct cgcggtcatg gcgagcggcc tcgcgctcac gtccgtgccg gcgtctgccg
     3661 agcgggaagg gacgcggcca gccgaggccg cgccgccgga cctgcgcgcg ctgcgtcagc
     3721 gcgcggagga gcggttccgg atcgtcgaga tccgaggggg gatgatcttg attccgagat
     3781 cggacgcggc cggcgtgcgc accatcgacg tgaacgacgg gcgggtgctg ctcgacggaa
     3841 ccgcggtaac aggccgcgag ttgcgggatc gcctcggcag ggacgcggac ctggtggccc
     3901 agctatcgtt tcttgacgcg gagggccgtc gtgcgttctt tgcagccgcc acagcggaga
     3961 ttccatcgcc ggagtccgag gcccctggcg ccccgggtca gccggccttg cccgacgtgt
     4021 ccggctggcc caaggcggct cggggccgcc atggcggcgc acgcgtccgg ataggcggcg
     4081 atgtgatcgt cggcagggac gaagcggtca gcgacgtggt ggtcgtcctg ggatcggcgc
     4141 gcgttgacgg gcttgtcgag agcagcgtcg tcgccgtggg cggctcggtg gttctcgggc
     4201 cgacggcgcg cgtccgcggc gacgtgacgt ccatcggcgg cggcgtcgag tgcgatccgg
     4261 gcgccgtcct ggaaggcgag agcaacgaga tcgcgttctc ggggccgcat ctcgggccgc
     4321 tgctccgtgt tcggccgcgg agggactggc gctgggtgat cgctccgttc gatggcaggc
     4381 cgagaggcac ggtcgatctg ctgaccacgc tcgttcgtct cgggctcgcg gcgctcttcg
     4441 cggcgatcgt cgcagcggcg gcgcccgggg cggtgagagg ggtctcggac cgcgccgcgg
     4501 cagaaccctg gcgcgcgggc ctcgtcggcc tggccgctga actgctgttc gtgccgctgc
     4561 tcgtgctgac ggtcgcggtg ctcgccatct cgatcatcgg gatcccgttg ctcctgctgg
     4621 tgccgttcgg cctcatcgcc gtcgggctgg cgttcgtgct gggcctggcc ggggccgggc
     4681 gcgcgctggg cacgtgcgtt gcccggcggg tgggcggcgc ggcgccgggc atgcccgcgt
     4741 cgctcgtcct cggcctcgcc gtgatctggg cgctgacggt cgtcgcgagg ttcgtcggcc
     4801 tgctcgggcc tccggctggc ttctttgtcg ccgtcctgct ggtggccggc ttcctcgtcg
     4861 agtacgtcgc gtggaccgtc ggcctcgggg ccgtcatcct gagccgcttc ggccggcgcc
     4921 ggagcgcgcc tgcggggccc ggcctctccg acgccgtggc gtaggccgcc aggttgcttc
     4981 ccgcgcgccg cgcaccgtcc gggccgcgac gcccggccgg gcggtgtgtc ttcgcgccca
     5041 cctcgtgcat aatcgactga tgcggtacac ggaacagtct ctccctgtcg gcgtgatcgc
     5101 gtcggcgtgg tcgtcgctcg acgtcgatct gctcgtcgtg ccggtcttcg agggcgaggt
     5161 gccggcgctc ccggccgacc tcgaggcggt ggtcgggccg cttgtccgcg cggtcatcga
     5221 gcgccgggag ttcaccccga agcccttcgc gacgctcgtg ctgccggccg ccgggccagg
     5281 ctggcgcccg gagcgcgtga tgctcgccgg ctttggaacg cgcgctgagt gcgacgccag
     5341 gcgcgtcagg cgagccgcgt cggccgccgc actcgccgcg cgcgagcggc gcgtccggcg
     5401 cctggccatg gcggcctccg gggcgctgcc gctggccgat gaagcccggg cggcggcgga
     5461 ggggctgacg ctggcgtccc actactcggg aatctacaag agcggcgacg aggcgcggcc
     5521 ggcgcccatc gaggcgctcg tggtggtgcc gcccgcgacg gcctccgccg acgtgcggga
     5581 ggccgagcgg gaggcggggc gcggctacat cctcgcgtcc tgcagcaacc tcgcgcgagc
     5641 cctcacgaac gagcctgcga acgtgatgac gccgaggctg ttcgccgaca tcgccggaca
     5701 ggtcggctcc gacgccggcc tccgagtcga tgtgctcgac gaagaccgga ttgccggcct
     5761 gggcatgggc ctcctgctcg gcgtctcgcg cggcagcgcg gagcctccgc gcgtggtggt
     5821 cctccgatac gacccgccgg acgcgccggc acggcccctg ctcggcctgg tcggcaaggg
     5881 cgtcacgttc gacaccggcg gcatttccat caagccctcg gatcggatgc acctgatgaa
     5941 gaccgacatg gcgggcggcg cggcggtgct gtcggccatg aaagccgtcg ccgcgctgaa
     6001 gccgaacgcg ccggtcatcg gggtggtgcc gcttgtggag aacatgccag gcgggcgcgc
     6061 gacgaggccg ggcgacgtcc tgcgcagcgc gtcggggaag accgtcgagg tcctcgacac
     6121 ggacgcggag gggcggctca tcctgggcga cgcgctgtgg tacgcgcgag agcttggcgc
     6181 gacgcatctg ctggacgcgg ccacgctcac gggggccatc ggcatcgcgc tgggccgcga
     6241 catgacaggc ttgttcggca cgcccgccca gtggaccgat ctcgtcgcga ggacggcggc
     6301 ccgggccgga gatcgcgtct ggcagatgcc cctggtcgac gagtaccggg aacagcttcg
     6361 cagcgagatt gcggacatct ccaacatcgg cggccggccg gccgggtcga tcacagccgc
     6421 gctcttcctg caggagttca gcggaggacg tccctgggcg cacctcgaca tcgcctccac
     6481 ggcctgggcg gatgaagcgc agccgtggca gccgaagggt ccgacgggcg tggcggtcag
     6541 aacgatggca gaactcgcct gcacgcctga cgcgtggccg ccgcctgtgg cctgattgcc
     6601 ccgctccctc gggcgcgccg gctaccggtt gggggagctg agatccagcg gcgtgtgatc
     6661 gctcggcagc gcgccgccgt ggcgcgcctc gcgccaccgc ttgatgccga tgagcacggc
     6721 gcccagcagg ccgccgacgg ccagcgcggc gatcacgatg acgccaacga ggccgagcga
     6781 gtcaagaatg acgtcggcgg cgccgacctg gggagtcggc tccgggacga ccttgacgat
     6841 gaccggggtg acgggctgcg ctgcgagcat cgacgacccg ctattgtagc ccctccagcg
     6901 cctggccccg caccggcccg ccgccggcgc cggcggcggt gtgcacgcgc ttcagcgtgc
     6961 ccaggtagcc gcggataccg tcgaggatcg cctcggcgat gcgctgccgg tagctcgagg
     7021 tgccgagcag ccgcccttcc tgccgatgcg tcagaaagga cacctccacc aggacgctgg
     7081 gcatggaggc gcctacgagg acgacgaagg gcgcctgctt gacgccgagg tccctgatcc
     7141 cctggttcga cgccctcagc cgcctcacga gcgagcgctg gaggtgctcg gcgagatccc
     7201 gggactcgtc cagcttgctg ttcagggcga tctgcttcac gagatcgttg aggtggctca
     7261 tcgtgagcga cgaggacgcg ttctcgcggg cggcgaccgc ggcggcctcc ggcgtggacg
     7321 cgaagttgag caggtagcac tcgacgcccc gggcgttgcg gttgcggctg gcgttcgcgt
     7381 gaatggagac gaacaggtcc gcgcccgcgc ggttggcgat ggcggtccgc tcgtgcagct
     7441 cgacgaactc gtcggtgcgg cgcgtgagca ccacctcgat gccgggctcg ccggagagca
     7501 gcttctcgag gcgaagggcg acgtcgagcg tcaggtcagc ctcgttgaag ccgggcccgg
     7561 cggctccagg gtcgcggcct ccgtggcccg cgtcgatcac gatgcgcccg acgcggagcc
     7621 cgagctgcct ggccatcgag tagccgccgc ggccgttggc cgatggcgcg gctggcgact
     7681 cggggcggac agccggagcg ggcgttggcg gcggcgccgg agccgcgccc cgcggcccgg
     7741 ggtccgccgg ctggacggga gcgcgcgcgg gctcgggcgg gcggtctgcc gcggccggct
     7801 ccgcgacgga accgacggcc gggacgccgg tctgaacggc gggctcgtcg cacgaaatga
     7861 tcacgcggta gggtgcggag gcgaggcggg acgagcaccg ggtcgccgcc cccgtctcga
     7921 tcacgatcct cgtgacgtcg ttcgggtgcc gtccgacgcg cagccgcttg atcgcgccgg
     7981 actcgaacgc gaacgaagcg tcgcggagcg cgtccatcgt gtgcgtgttc gcgagatcca
     8041 cgaagaggcg cggcggctga tcgaggcgct gttcgcgaaa ctgaacgggc ccgtccagtt
     8101 cgacttccac cgaggccccg cctggcgtca cgaggcgccg gatgtcgcgg acgaggacgc
     8161 ggccccgggg agccgaactc gccgcggcgg cgccaggcgg cgcaggcgcc gcctggccag
     8221 cgtccgccgc cggggccgcc ggggcggcga gtccccgcag cctcgcagcc tccgcgcgcg
     8281 cacgcgccgc cagcgaactc gacgggtact cgcgcgcgag cgaggtcagg agggtcaccg
     8341 cgctggcgcg gtcctggtcg ttgccgtaac gggagaacgc ctcggccaga agctgcgcgc
     8401 cgagccacag agcatcgtcg cagtatccgc tccgcgggta ctgcagcacc acctggcggt
     8461 aggccgcgac cacctgccga atcgcgtccc gcgtcggcgc cggccccggg ggtgaggcgg
     8521 ccagcgcctg gcgcagcgcc tcctcgcgcg ctgccgccgc ctgaaacaag cggcgggctc
     8581 cctcctgcgg gtcggccggc gcgggcgcgg ccgaaccgga cagtccggcc gcggccaggc
     8641 acaccgccgc cagggcgtat cggacggccg gccggccgcg ggcgcgtgaa ggcagtccag
     8701 gcatgtcggg cgctaccgca gctgctgatc cacctcgtcg aagtacccct tgtccaccag
     8761 cacctctctc ggcttgccgc ccatcgcggg cgacacgatg ccctcggcct ccatcatgtc
     8821 gatcagccgc gccgcccggc tgaagccgac gcggagccgc cgctggatgt acgagatcga
     8881 ggcctggccg gtcgagacca ccaggcgcgc ggcttcgtcg tacaagtcgt ccttttcgaa
     8941 ctcgatcgcg tcggcagcct tttcgtcggc gatgatcgac gtgtcgtacg taggcttgcc
     9001 ctgcttgcgc aggaacgagg ccagccgcgc cgtttcctgc tccgagatgt aaggcccgtg
     9061 cacgcggacg aggcgcgacg agtccggcgg cgagaacagc atgtcgcccc gcccgaggag
     9121 ctgctccgcg ccgttcccgt cgatgacggt gcgcgaatcg accttcgacg agacgcggaa
     9181 cgaaatgcgc gcaggcagat tggccttgat gagccccgtg atgacgtcca ccgacgggcg
     9241 ctgggtcgcc agcaggagat gaatgccgat ggcgcgggcc atctgcgcga gacgggcgac
     9301 ggattcctcg acctcgccgc gcgccaccat catgaggtcc gccagctcgt cgatgacgac
     9361 gatgatgtag ggcagcggct tggggagcgg ctcgtccgcg gacgaggagc gctcgagcac
     9421 cgcctgccgc acgttgcggt tgtactggtc gatgttccga acgccctcgg cagccagggt
     9481 cttgtaccgc tcctccatct cgcggacggc ccaggtcagc gcgttcgccg cctgcttggg
     9541 gtcgaccacg acgggcgtca ggaggtgcgg tatctcctcg tacatgccca gctcgaggcg
     9601 cttgggatcg atgaagatca tccgcacgtc ctcgggcgtc gcgcggaaca ggatgctcgt
     9661 gatcatcccg ttcagcgcga cggacttccc ggtcccggtc gccccagcga tgagcaggtg
     9721 cggcatcgtc gccaggtcgg ccacgaaggg ctccccctgg atcgtcttgc cgagggccag
     9781 cgtcagcttc gagggcgacg cctggtaggc cgccgactcg agcatctcgc gcagcgagat
     9841 cgtctcccgg aactggttcg ggatctggat gcccaccgtg gacttgcccg gcgtccgctc
     9901 gatcaggacc gactcggcct tcatggcgag cgagagatcg tcggccaggc ccgtgacctt
     9961 gctgtacttg actccggcct cgggcttgaa ctcgaacgtc gtcacgaccg ggccagggtg
    10021 gatttggacc accgatcccg cgacggcgaa ctcgcggcac ttctcctcga gcaatcgcgc
    10081 agactccatc agctcgcgct cgtcgatctt gcgctcggtc cgcggcgcgt ccagcagcgt
    10141 ggagggcggg aaggcatagc cgttgcgctt gcgttcggcg ggggatcgag ccggcgggtc
    10201 ggcggccggg ggcgcggggg ccggagggcc gaccacggta atgggggtgt ctttccgctg
    10261 gcggggccag cttgaggcga gggccgaccc gttgcggtgc gctgatgcgg cgttcgggtc
    10321 ggcgacggcc gcggaggcgg gctgggcccg gcggccgttg ggccccgcct cggcgccagg
    10381 cggccgggag cccctggctg cgtcggtggt cccggcacgc ctcgcgtgct tcgagacgat
    10441 ctcgcggcgc tgtcgctcgc gcctccgcgc atcgcgccac gtccagaacg ccgcgacgcg
    10501 cgtcgccgcc cgttcgcggg tccaggacgc gatggcggag aaggcgcggc cgaacgagaa
    10561 ctgggtcgtc aagacgaggc tcagcccgag gcacgccagg atgacgatga gcgagccggt
    10621 gcggttgaac gaggctacga gcgcaccggc gaactggtcg ccgagccagc cgccgggctc
    10681 gaacgacgcc ccggtcgttc gtgcgggcgc cagcgcgagg ctcaggaacg acgacgcgga
    10741 gccgacgagc agcagggccc cgatggcctt cgtgtagcgc gcctcgatgg gccggcacca
    10801 gaactggcgc cagccgacgg cgccgaccag cagcggaaac agccaggcgg catatcccac
    10861 gacctggtgc gagagctccg cgacgaaagc cccgacgcgc ccagccaggt tggctgccgg
    10921 tgcggacgac ccggtgttga agaaccatgc gggatccgcg ggggagtagg tcgccagggc
    10981 gatgagccac agcaggccca cggcgaatcc cgccacgccc aggaattcgc tgagccgccg
    11041 cgacagggtg gatgaggcgc tcaccgacta gatctccatg atcaccggga gcacgagggg
    11101 ccgctgcccg acgcgcttcc ggaagaagcg gcgcagttcg acgccgagct tgtcgcgcat
    11161 gatcccgtag tcagtgcgct cctcgacgct ggccgaatcc acgatgtccg caatcaggcg
    11221 ggagaggtcc cggaagggct gctcgtcgcc ttgcgggacc acgatgccgc gggtgatgac
    11281 ctcgggcacg ccctcgacgg cgcccgtctg cttgttgatg gcgagcacgg gcacgatcag
    11341 gccgtcttct gccaggtgcc ggcggtccct gaggatctcg tcgcccaggc ccgtcacccc
    11401 ggcgccgtcg atgagcacgc ggccggacgg cacccggccc gcgacgcggc acccgccggc
    11461 gtcgaaatgc agcacgtcgc cgttttccgc gacgaggacg tccatgctcc ccgggacgct
    11521 gcgggccagc cgctcgccga cccgcgcgtg gcgcgcccgc tgccggaagg tgccgtggat
    11581 gggcaggaag cagcgcggcc gcaccagcga gagcaggagc ttcagctcct cctcgctgcc
    11641 gtggccagag acgtgaacgt gcttggtctg ctgattgaca atctcggcgc cccggcgggc
    11701 gaggtggttc actacgcggt cgatctggcg ctcacgaccg gggatttcgc gcgccgagaa
    11761 cacgaccacg tcgcccgggc cgagccgcac gtgccggtgg tcgtcgatgg cgatgcgcga
    11821 cagcgcggac atcggctcgc cctggctgcc ggtgacgagg cagagcacct gcgacggcgg
    11881 cagcgccttg acgtccgcat cgcggacggc caggcccggc gggaccgaga ggtagccgag
    11941 acgctgcgcc agctgcgacg tctcgaccat gccgcgcccg atgaacgcca ccttccggcc
    12001 gaacctggac gcgaggtcca cgaggatctg caggcggtag acgctcgtcg cgaacgccgt
    12061 gacgacgagc ttgccccgcg tgctcgcgaa gatctcctcg aacgccggaa tcacggagag
    12121 ctccgagccg gtgacgcctg
//
LOCUS       DAOSIF010000032        11904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5380_length_11904_cov_4.419951, whole genome shotgun sequence.
ACCESSION   DAOSIF010000032 DAOSIF010000000
VERSION     DAOSIF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5380_length_11904_cov_4.419951"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLT35_02200"
     CDS             complement(<1..1106)
                     /locus_tag="PLT35_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28067.1"
                     /translation="MKICVLQPSYEGSSCDYRHYDPPRDLAPLLPEHEFHHAFLDKIS
                     TYAQLKALARQGFDIFVNLCEGYPDSDVPGIDVVIALEQLKLPFTGPPSALYDQPKDV
                     MKLVAVSSGVTVPRWVLARTEADVAAAEAALRFPLFVKPAGYGDSMGIDERSLAATPA
                     GLRRQAAKRLPEYGSLMIEEYVEGREFTVLVCGAPDPFQPPVALRPLEFVFPPGHRFK
                     TYKLKVTQFHPECNVPCEDAALAGRLKDAAIRVFTGFSGEGYARLDFRVAPDGTVYFL
                     ETNFTCSVFYPDGFQGSADYILAHDGLGQAGFLRGIIAEGLARHARRQPPYAVRQNGS
                     GFALYAARDVPAGGVVFEGEGRAQRIVTRAHVERA"
     gene            complement(1110..2225)
                     /locus_tag="PLT35_02205"
     CDS             complement(1110..2225)
                     /locus_tag="PLT35_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28068.1"
                     /translation="MSVPDRFDGWQPVVSVFIPYYDKDGQLESPEYDLPGFRAELDGW
                     FAELGYEWRWVPVTLNNLAGVVAELKGPRREKRCLVMNLCDGNELDGSPGVSVVKVLE
                     QAGLPFTGSTSAFYEITTYKVPMKLRLLERGVPTAPFVPLRSLPEDIARLEAEVGYPA
                     FVKPEVSAGSGGIGLTSLVTDAEGVAARVAWLLASEDGDFYRRSGIFAERFIDGPEFT
                     VLVVGDRGAPGGARAYPPAQRLFHSALPAHERFLSYDRYWSEFKYEPRLPEGEPFYRF
                     GKAPAHLCDRLAALAVQAFLAVDGAGYGRVDIRLEERTGKLFVLEVNSNCGLTGDQET
                     SVGEILLQAGIPATVLVSEILRDAYDRHVSSGRRRTA"
     gene            2422..2730
                     /locus_tag="PLT35_02210"
     CDS             2422..2730
                     /locus_tag="PLT35_02210"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOG28069.1"
                     /translation="MAFLTGKGSAEALRAAGSLSTVGLAFVLALAIGFWFGSVLDRWA
                     GTRPLFTIVCFFFGLAAGILNVYRIVSRAFPAGRPAPGAPAPPADPGPSDRDLHADDD
                     "
     gene            2750..3193
                     /locus_tag="PLT35_02215"
     CDS             2750..3193
                     /locus_tag="PLT35_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28070.1"
                     /translation="MTVLRETDPSLRRVQRDSAIIGAALALAALVLQRGRPGGALGVL
                     AGVALMAVSYRAISGGVSAIVRRAEAAAGSAAGSGAPKVRTSLVFAGFIGRYAVVGAL
                     AWAVLVPLRADPVGLVAGVSVTVAAIAVEAVRLVRTGRRAGPPGA"
     gene            3323..4138
                     /gene="atpB"
                     /locus_tag="PLT35_02220"
     CDS             3323..4138
                     /gene="atpB"
                     /locus_tag="PLT35_02220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG28071.1"
                     /translation="MEKLEHELWIVQAANAVFGRMVNAFLGLFGLQAADPAHPIPNYL
                     VMVFLIAALLVALAFLLRSRLSVENPGRLQIVFEDAVNALGGMLDDIVGPKGRRYLPL
                     VATVGVFILCGNLIGLVPGFMAPTSSINVTLGCALTVWIYYHIQGVREQGLLAYLKHF
                     AVPPGAPAFIAPIMLPIEIISHLSRVLSLSLRLFGNIFGEELVIVILFSIVPFVVPLP
                     MMFLGIITGSLQAFIFVMLTMIYLSGAVAAEHGHEDAHAHAHRPARHAEGVAA"
     gene            4182..4517
                     /locus_tag="PLT35_02225"
     CDS             4182..4517
                     /locus_tag="PLT35_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOG28072.1"
                     /translation="MRFALMFTFVVLTLGLASPAYAQEAAGAAIGVEVVRWSIITAGF
                     ALAIAATFGALSQAKGLSAAVEGIARNPAAANDIRGNLILGLVLIESLVIYVLLISLI
                     LFFIKPFGS"
     gene            4647..5561
                     /locus_tag="PLT35_02230"
     CDS             4647..5561
                     /locus_tag="PLT35_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG28073.1"
                     /translation="MRRSLPLSPFDALLLLMVIIWGSNYSVVKAALREIPPQAFNAAR
                     MAVASAVFAAALAWTGLPRLPKRDWIRIAAVGAIGHFVYQLFFISGIARTSASNSSLI
                     IGCSPVAVSIASALAGHERIAPAQWAGILISLAGVYLVVGLGAGFGGASLAGDLLTMG
                     AVVCWAVYTVGSRTLLTRYSPLTVTGLTMLCGTMLLVPASLPAMSRVAWTSVPAWAWV
                     ALVLSALLAIDLAYLIWYTSVQRIGNVRTSAYSNFIPLVAMAVAIVALGESLTVLKAA
                     GAAAILGGVAITRTAAKSRAMADPPGEE"
     gene            complement(5670..7805)
                     /locus_tag="PLT35_02235"
     CDS             complement(5670..7805)
                     /locus_tag="PLT35_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HOG28074.1"
                     /translation="MQTTTAQDPARDVQAGRPIDLPHETLLWAYRIMYLSRKMDDKEI
                     QLRNQSQIHFQISGAGHEAILTAAGRHLRAGYDWFFPYYRDRALCLALGVTPYEMFLQ
                     AAGAADDPASRGRQMPSHWGHRDLHIVSQGSPTGSQCLHAVGCAEAGLLRGRLPGLAD
                     REAGRRDDEVSYVSLGDGTTSEGEFWESLNTACNLRLPVVYLVEDNGWAISVPVEVQT
                     AGGSISALLRSFPGLLLVSADGTDYLDSDRAMREALVYARARKGPALVHAQVIRPYSH
                     SLSDDERLYKTEAERDAERRRDPLARMAALLLSRGAATEESLDALRASVAREVDDAAK
                     RAIAAAKPAPETAALWVFSPDVDPSSDAFDTPARPEGRPDTMVAAINRTLKDEMARDP
                     RIVVFGQDVADCSRERSLDEVQGKGGVFKVTHGLQRQFGSDRVFNTPLAEANIVGRAV
                     GMAVRGLKPVVEIQFFDYIWPAMQQLRNEASMLRYRSGNAWSCPMVVRVPIGGYLRGG
                     APYHSQSGVSIFAHCPGIRLVFPSNAADAAGLLRTAIRCDDPVMFLEHKHLYRQTYNK
                     GEYPGPDCTIPFGRAAVRREGRDVVIVTWGALVQRSILAAQQAEKEGLSVAVLDLRTL
                     APCDWDAIGAWVARTSRVIVAHEDQLTCGFGAEIAARIAQEHFHDLDAPVRRVAALDT
                     PVAYSPILEEAILPGPAGLLQAIRETAEY"
     gene            8005..8796
                     /locus_tag="PLT35_02240"
     CDS             8005..8796
                     /locus_tag="PLT35_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG28075.1"
                     /translation="MHAGLTGKAALVCAASRGLGKAAARGLAAEGASVAICARSQEPL
                     EAAAEEIRAETGAVVLALVADVSRQADVVRVVDETVRRLGRLDILVTNSGGPKAGLFG
                     AISDADWRDAVDLVLLSAVRLCSEAVPHMRRVGGGRIVHITSVSAKQPIPGLMLSNAL
                     RPAVTGFAKTLAAELARDNILVNCVAPGYTRTERVVALAQAAAAREGAALDTIQARNI
                     QEIPLGRMAEPEDIADAIVFLASERSRSITGTTLAVDGGYLRGLL"
     gene            complement(8744..10141)
                     /locus_tag="PLT35_02245"
     CDS             complement(8744..10141)
                     /locus_tag="PLT35_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28076.1"
                     /translation="MRSGRFFAVAVLAGLAVRLAALPLPGTSDVTAFKVWAHAAATGP
                     VAEMYGVGGTPPERRVHEYDHWEATVDYPPVALYALSAAGRLYGWARPGFANTPLLAA
                     CIKALPLACEGAMTLVLWAAVRRVQPHRADRARFAALAFWLNPASILATPVLGYLDAL
                     FALPALGALVAAASGAAGLSGALMAAAILTKPQAVLFAPAVALALAVSPSGGASRPRA
                     RSLSLAAGAAILSAAAILAPIAAAGAWPNFVQAMRSLGRHDMLSGQAANLWWIVTYIM
                     RAAYAAAEMGVAGAFLSPVRRPLAITRLAELGYPDPRLAATALTVAAVAWALWTGRKV
                     RDLPRFSLLGAWSVYAYFVLSVQVHENHFFMILPPLALAAALLPRWRAPLWVLSGMFA
                     LNLNLFYGFGDGIGFAVPRTATRIDATVLLSAANLAAFAWVARLLRDTLRDAPASGAT
                     ADREDSRRRPRALFP"
     gene            complement(10154..10915)
                     /locus_tag="PLT35_02250"
     CDS             complement(10154..10915)
                     /locus_tag="PLT35_02250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG28077.1"
                     /translation="MQYRPSRLSYASGPGPMTPAVRALLVVTTAGYLITLAVPGLVIR
                     LGLVPALVLERGWFWQPLTYLFQHGWLFHLVFNLLALWMFGVDLERRWGTRFFVRYYA
                     VTGAGAALTTIGVSLLPFGFSQAIYASVTVGASGAIYGLLVGYAMMYPDRPIYLYFLF
                     PIRARVFVLIVGAISFLSSVSDPRGGVAHATHLGGLVVGYLYLAWRRGGLGSALRSQW
                     LRWRMARLRGRFDVRDGGRSGRGGRPGNGGGGWVH"
     gene            complement(10917..>11904)
                     /locus_tag="PLT35_02255"
     CDS             complement(10917..>11904)
                     /locus_tag="PLT35_02255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG28078.1"
                     /translation="RWRPHAFLVAGPIVSVTVLSLVGMAWTPATLAGVLVIGGLGGAA
                     FHPTAAAVVHRQGGSRRGLAMAIHITGGSIGYAVGPLLFAPYVGRFGLTSTPLLALPG
                     LALLALVLRGMPLMRPLASGSGGGYLALRPFAKPLFLLWLIVVLRTMTSLSIATFVPV
                     LLTTRGWSVGMAGVAVSVYLAAGGLGGFAGGPLADRLGPKRVIAGSLVLAAPLLAAAT
                     RLTDWPLVAVLALGGFFLQSTLPVNVTFAHQIAPTGAATVSSLMLGFAWGTGGLMAPI
                     VGAAADRIGIGTTLTAVAFLPLLAAACSMPLPSGAKTESAVRRPDERALGRV"
BASE COUNT         1767 a         4202 c         4269 g         1666 t
ORIGIN      
        1 gcgcgctcca cgtgggcccg cgtgacaatg cgttgcgccc ggccctcgcc ttcgaagacc
       61 acgccgcccg ccgggacgtc gcgcgcggcg tacagcgcga agccggagcc gttctgcctg
      121 acggcgtagg gcggctgccg gcgcgcgtgg cgcgcgaggc cctcggcgat gatgccccga
      181 aggaagccgg cctgcccgag gccgtcgtgc gccaggatgt agtcggccga gccttggaac
      241 ccatccgggt agaacaccga gcaggtgaag ttggtctcga ggaagtagac cgttccgtcg
      301 ggagcgaccc ggaagtccaa gcgcgcgtac ccctcgcccg agaaccccgt gaagaccctg
      361 atggccgcgt ctttcaggcg gccggccagg gccgcgtcct cgcagggcac gttgcactcc
      421 ggatggaact gcgtgacctt cagcttgtac gtcttgaacc gatggccagg ggggaacacg
      481 aactcgagcg gacgcagcgc caccggaggc tgaaagggat cgggcgcgcc gcagacgagc
      541 accgtgaact cgcgcccctc cacgtactcc tcgatcatca ggctgccgta ctcgggcagc
      601 cgtttggcgg cctgccgcct cagcccggcg ggcgtggccg ccagcgaccg ctcgtcgatg
      661 cccatgctgt cgccgtagcc ggccggcttg acgaagagcg ggaagcgcag cgcggcttcg
      721 gcggcggcga catccgcctc ggtcctggcc agcacccacc gcgggaccgt cacgccggag
      781 gacacggcca cgagcttcat cacgtccttc ggctggtcgt agagcgcgct gggcgggccg
      841 gtgaacggaa gcttcagctg ctccagcgcg atgaccacgt cgatccccgg gacgtccgag
      901 tcggggtagc cctcgcacag gttgacgaag atgtcgaaac cctgccgcgc gagcgccttc
      961 agctgggcgt aggtcgagat cttgtccaga aaggcgtggt ggaactcgtg ctccggcaga
     1021 agcggcgcga gatcgcgggg aggatcgtag tgccggtagt cgcaggacga accctcgtac
     1081 gacggctgga gcacgcagat cttcatgagt caggccgtgc ggcgccgccc gctcgagacg
     1141 tgccggtcgt aggcgtcccg gaggatctcg gagacgagga cggtcgccgg gatgccagcc
     1201 tggagcagga tctcgccgac cgacgtctcc tggtccccgg tgaggccgca gttcgagttg
     1261 acctcgagca cgaacagctt gccggtccgc tcttcgagcc ggatgtcgac gcgtccgtat
     1321 cccgcgccgt cgacggccag aaacgcctgc accgccaggg cggccagccg atcgcacagg
     1381 tgcgccgggg ccttgccgaa gcggtagaag ggctcgccct cgggcagccg aggctcgtac
     1441 ttgaactcgg accagtaccg gtcgtagctg aggaaccgct cgtgcgcggg cagcgccgag
     1501 tggaagaggc gctgcgcggg cggataggcg cgcgcgccgc ccggcgcgcc gcggtcgccc
     1561 acgacaagca ccgtgaactc gggaccgtcg atgaaccgct ccgcgaagat gcccgagcgg
     1621 cggtagaagt cgccgtcctc gctcgcgagc agccacgcca cgcgggcggc gacgccttcg
     1681 gcgtcggtca ccagggacgt caggccgatg ccgccgctgc cggccgacac ttcgggcttc
     1741 acgaaagcgg gatagccgac ctcggcttcg agcctcgcga tgtcctccgg caggctccgc
     1801 agcggcacga acggcgcggt cggcacgccc cgctcgagca gccgcagctt catcgggacc
     1861 ttgtaggtcg tgatctcgta gaaggccgaa gtactgccgg tgaacgggag gcccgcctgc
     1921 tcgaggacct tcacgaccga cacgcccggc gagccgtcga gctcgttgcc gtcgcacagg
     1981 ttcatcacga ggcagcgctt ctcgcgacgg ggtcccttga gctcggccac gacgccggcg
     2041 aggttgttga gggtgacggg cacccaccgc cactcgtatc ccagctcggc gaaccagccg
     2101 tcgagctcag cgcggaaccc gggcaagtcg tactccgggc tctcgagctg gccgtccttg
     2161 tcgtagtagg ggatgaagac ggacacgacc ggctgccacc cgtcgaaccg atcaggcacg
     2221 ctcattgcgg accggcgcca cctcctcgcg cgcgaaccga gccacgatac tagcgtccga
     2281 ccgcgccgcg cacaagcgga gaatgcacgc ggcgcacgcc gcgtcggtcg ctgcccgcgc
     2341 aggcgccggg ctcgtggatg gcgcgcgcgg ctcggccttg atgggcgggg ccgcttggac
     2401 gatactggca gcggtgccgc catggcgttc ttgacgggaa agggcagcgc ggaggcgctg
     2461 agggccgcgg gatcgctcag tacggtgggc ctagcgttcg tgctggcgct tgcgatcggc
     2521 ttctggttcg gctctgtgct cgaccgctgg gccgggacgc ggccgctctt cacgatcgtc
     2581 tgcttcttct tcggcctcgc cgccggcatc ctgaacgtgt accgcatcgt gtcgcgggcg
     2641 tttccggccg ggcgtcccgc gccgggtgcg ccggcgccgc cggccgatcc tgggccgtcg
     2701 gatcgcgatc ttcacgcaga cgacgactga gcccgcttgg gaccgccgca tgacggtgtt
     2761 gcgcgaaaca gacccctcac tgcgccgtgt gcagagggac tcggcgatca tcggcgccgc
     2821 gctcgccctg gcggcgctcg tgctgcagcg cgggcggccg ggtggcgcgc tgggcgtgct
     2881 cgcgggcgtg gccctgatgg ccgtcagcta ccgtgccatc agcggggggg tctcggccat
     2941 cgtccggcgg gccgaagcgg cggccggctc tgccgccggc tcgggcgcgc cgaaggtgcg
     3001 gaccagcctg gtcttcgccg ggttcatcgg gcgctacgcg gtcgtcggcg ccctcgcctg
     3061 ggcggtgctg gtgccgcttc gcgccgaccc ggtgggactg gtcgcgggcg tctccgtcac
     3121 cgtggcggcc atcgccgtgg aggccgtcag gctcgtccgg acggggcgcc gggcggggcc
     3181 cccgggcgca tgagccgcgc gcccggacgg ggcgcgtccg ctttaccgga cctgccgcca
     3241 tacgatatcc tcttaccttg gcatggctcc tccgggagcc ggctccgagc accacggccc
     3301 ccccagccgg atcgcctcga ctatggagaa actcgaacac gaactctgga tcgtgcaggc
     3361 ggccaacgcg gtcttcggcc gaatggtgaa cgcgttcctg ggtctgttcg ggctccaggc
     3421 ggcggacccg gcccacccga tcccgaacta cctggtgatg gtgttcctga ttgcggccct
     3481 cctcgtcgct ctggcgttcc tgctgcggtc caggctgagc gtcgagaacc ccggcaggct
     3541 ccagatcgtc ttcgaggacg ccgtcaacgc ccttggcggg atgctcgacg acatcgtcgg
     3601 ccccaagggc cgccggtacc tgcctcttgt cgccacggtc ggcgtgttca tcctgtgcgg
     3661 caacctgatc gggctcgtcc ccggcttcat ggcgcccaca agcagcatca acgtgaccct
     3721 tggctgcgcc ttgacggtgt ggatctacta ccacatccag ggcgtcaggg agcagggcct
     3781 gctggcctac ctgaagcact tcgccgtgcc gccgggcgca ccggcgttca tcgcgccgat
     3841 catgctgcct atcgagatca tcagccacct ctcccgcgtg ttgtcgctgt cgctgcggct
     3901 gttcggcaac atcttcggcg aggaactggt catcgtgatc ctgttcagca tcgttccctt
     3961 cgtcgtgccc ctgcccatga tgttcctggg catcatcacc ggcagcctgc aggcgttcat
     4021 cttcgtcatg ctgacgatga tctacctgag cggcgcggtg gcggccgagc acggccacga
     4081 ggacgcacac gcgcacgcgc accgaccggc ccgccacgcg gaaggcgtgg cggcctgaca
     4141 cccggacgtc gaacccaact gaggaggctg tggtcgtgaa gatgcgtttc gctctgatgt
     4201 tcacctttgt cgtgttgaca ctcggcctgg cgagtccggc ctacgcgcag gaggcggcgg
     4261 gggcggcaat cggcgtcgaa gtcgtgcggt ggtcgatcat caccgccggt tttgccctgg
     4321 cgatcgcggc gacgttcggc gcgctgtcgc aggccaaggg cctcagcgcg gcggtggagg
     4381 gcatcgcgcg caatcccgcg gcggcgaacg acatccgcgg caacctgatc ctgggcctgg
     4441 tgctgatcga gtcgctcgtg atctacgtgc tgctcatctc gctgatcctg ttcttcatca
     4501 agcccttcgg ctcctaggcc cggccgcccc gggtccgccg ccggccgcgc tccgggcgtc
     4561 ccggcccccg ccgggatgtc cggcgctcgt cgggcgcggc ggccctgacg cggcgcctcg
     4621 gcgcgcgttc cgcgcggagg aagcgcgtgc gccgctcgct gcccctgtcc ccgttcgacg
     4681 cgctgctgct cctgatggtg atcatctggg gctcgaacta ctccgtcgtc aaggcggccc
     4741 tccgggagat ccccccgcag gcgttcaacg ccgcgcggat ggcggtcgca tcggccgtgt
     4801 tcgccgccgc gctcgcctgg accggcctgc cacgcctgcc gaagcgggac tggatccgca
     4861 tcgcggcggt cggggcgatc ggccatttcg tctaccagct cttcttcatc tcggggatcg
     4921 cgcgaacgtc ggcttccaac agttcgctca tcatcggctg ctcgcccgtc gccgtgtcga
     4981 tcgccagcgc gcttgcaggg cacgagcgga tcgcgccggc gcagtgggcc gggatcctga
     5041 tctcgcttgc cggcgtgtac ctcgtcgtcg ggctcggggc cggattcggc ggggcctcgc
     5101 tggccgggga cctgctcacg atgggggccg tggtgtgctg ggccgtctac acggtcggat
     5161 cgcgcacgct gctgacccgc tactcgcccc tgaccgtcac gggcctgacc atgctgtgcg
     5221 gcacgatgct gctcgtgccc gcttcgctgc cggccatgag ccgggtggcc tggacgagcg
     5281 tgccggcgtg ggcctgggtc gcgctcgtcc tctcggcgct gctcgccatc gacttggcct
     5341 atctcatctg gtacacctcg gtgcagcgca tcggcaacgt ccgcacctcc gcctactcga
     5401 acttcattcc gctggtggcg atggccgtcg ccatcgtggc gctgggcgag tcgctgactg
     5461 tcctgaaggc ggcgggcgcc gccgcgatcc tgggcggcgt cgcgatcacg cggaccgcgg
     5521 ccaagagccg cgcgatggcg gatcctccgg gggaggaata gcggtcccgg ctcccgccgt
     5581 cggccgacgg cccgcggtga acgaacggct gtggatcggc gggccggccc cggcggatgg
     5641 gcccgaatcc cgattcccgg gccccgtatt cagtactccg ccgtttccct gatggcctgg
     5701 agcagcccgg cggggccggg caggatggcc tcctcgagga tcgggctgta ggcgacgggc
     5761 gtgtccagcg ccgccacgcg ccgcaccggc gcgtcgaggt cgtggaagtg ctcctgggcg
     5821 atgcgcgccg cgatctcggc gccgaacccg catgtcagct gatcctcgtg cgcgacaatc
     5881 acgcggctcg tgcgggccac ccacgcgccg atggcgtccc agtcgcaggg cgccagcgtg
     5941 cgcaggtcca gcaccgcgac gctgaggccc tccttttccg cctgctgcgc ggccaggatc
     6001 gagcgctgca cgagcgcgcc ccaggtcaca atcacgacgt cgcggccctc ccgtctgacg
     6061 gcggcccgcc cgaacggtat cgtgcagtcg gggccggggt actcgccctt gttgtacgtc
     6121 tggcggtaca ggtgcttgtg ctcgaggaac atgaccgggt cgtcgcagcg gatcgcggtc
     6181 cgcagcagcc cggcggcatc cgccgcgttc gatggaaaga ccagccggat tccggggcag
     6241 tgcgcgaaga tgctgacgcc cgactggctg tggtacggcg cgccgccgcg caggtagccg
     6301 ccgatcggca cccggaccac catcgggcac gaccacgcgt tgcccgaacg gtaccgcaac
     6361 atcgacgcct cgttgcgcag ctgctgcatc gcgggccaga tgtagtcgaa gaactggatc
     6421 tccacgaccg gcttcaggcc gcgcacggcc atgcccaccg cccgcccgac gatgttcgcc
     6481 tcggcgagcg gcgtgttgaa gacccggtcg ctgccgaact gccgctggag gccgtgggtg
     6541 accttgaaga cgccgccctt gccctggacc tcgtcaaggc tccgctcgcg gctgcagtcc
     6601 gccacgtcct ggccgaagac gacgatgcgc gggtcgcggg ccatctcgtc cttgagcgtg
     6661 cggttgatag ccgccaccat cgtgtccggc cggccctcag gcctcgccgg cgtgtcgaac
     6721 gcgtcagagg agggatcgac gtccggcgag aagacccaca acgcggccgt ctccggcgcc
     6781 ggtttcgcgg cggcgatcgc ccgcttcgcc gcgtcgtcca cctcgcgcgc cacgctggcg
     6841 cgcaaggcat cgagggactc ctcggttgcc gcgccgcgcg acagcagaag ggccgccatc
     6901 ctggcgagcg gatcgcgccg gcgctcggca tcccgctcgg cctcggtctt gtacagccgc
     6961 tcgtcatccg agagggagtg cgagtacggg cggatcacct gggcgtggac cagggccggg
     7021 ccctttctgg cccgggcata aaccagagcc tcgcgcatgg cccggtcgct gtcgaggtag
     7081 tcggtgccgt cggcggacac gagtagcagg ccagggaacg agcgcagcag ggccgagatg
     7141 ctcccgcccg ccgtctgcac ttccaccggg accgagattg cccacccgtt gtcctcgacg
     7201 agatacacga ccgggaggcg caggttgcag gccgtgttca gcgattccca gaactcgccc
     7261 tcgctcgtcg tgccatcgcc gagcgatacg tacgagacct cgtcgtctct gcggccggct
     7321 tccctgtcgg cgaggcccgg gaggcgcccg cggagcaggc ctgcctcggc gcagccgacg
     7381 gcgtgcaggc actgcgaccc cgtggggctg ccttgcgaca cgatgtggag gtcccggtgg
     7441 ccccagtgcg aaggcatctg ccggccgcgg gacgcggggt cgtctgccgc gcccgccgcc
     7501 tgcaggaaca tctcgtacgg cgtgacgccg agcgccaggc agagcgcccg gtctcggtag
     7561 tacggaaaga accagtcgta gccggcccgg aggtgacgcc ccgcggccgt gaggatggcc
     7621 tcgtgcccgg cgccgctgat ctggaagtgg atctggctct ggttcctgag ttggatctcc
     7681 ttgtcgtcca tcttccgcga caggtacatg atccggtagg cccagagcag ggtctcgtgc
     7741 gggagatcga tgggccggcc ggcttgcacg tcccgggccg ggtcctgggc tgtggtcgtc
     7801 tgcatgctgc atcctgccgg agccgccgcg ggccggtggt tgggtgcgcg cgaatcggaa
     7861 tggcggaaac tgaaattata ccacccacgc ctgcgtggta tggtggaccc ggcgcagccc
     7921 gccgggacgc ccaaccggcc ggcgcaggcg gacgagcgcc gtgccgcgac gaggcgcatg
     7981 ccgggcgcgc gagaggagac gcggatgcac gccggactga cgggcaaggc ggccttggtg
     8041 tgcgcggcga gccgagggct gggaaaagcc gccgcccgag ggctcgcggc agagggcgcg
     8101 agcgtcgcga tctgcgcccg cagccaggag ccgctcgagg ccgccgccga ggagattcgg
     8161 gccgagacgg gagcggtcgt gctcgcgctg gtggccgacg tgtcgcgcca ggcggacgtt
     8221 gtcagggtcg tagacgagac cgttcgccgc ctcggacgcc tggacatcct ggtcacgaac
     8281 tccggcggac cgaaagccgg gctcttcgga gccatcagcg acgccgactg gcgagacgcg
     8341 gtcgatctgg tcctgctgag cgcggtgcgg ctctgctcgg aggccgtccc gcacatgcgt
     8401 cgagttggag gcgggcgcat cgttcacatc acgtcggtgt cggccaagca gccgataccc
     8461 ggcctgatgc tgtcgaacgc cttgcggccg gccgtcaccg gcttcgccaa gaccctcgcg
     8521 gcggaactgg cgcgcgacaa catcctggtg aactgcgtcg cgcccggcta cacgcgcacc
     8581 gagcgcgttg tcgcgctggc ccaggcggct gccgcgcgcg aaggcgcggc gctcgacacg
     8641 atccaggcgc ggaacatcca ggagatcccg ctcgggcgca tggcggaacc cgaggacatc
     8701 gccgacgcga tcgtctttct cgcctccgag cgcagccgtt cgatcacggg aacaacgctc
     8761 gcggtcgacg gcggctatct tcgcggtctg ctgtagcgcc cgaggcgggg gcgtcgcgca
     8821 gggtgtcgcg caggaggcgc gcgacccagg cgaacgccgc gagattcgct gccgacagca
     8881 gcaccgtcgc gtcgattcgc gttgcggttc gcggcacggc gaagccaatg ccgtcgccga
     8941 acccgtagaa gagattcagg ttcagcgcga acatgccgct cagaacccac agcggcgccc
     9001 gccagcgcgg gagcagggcc gcggcgagcg ccagcggcgg caggatcatg aagaagtggt
     9061 tctcgtgaac ctgcaccgag agcacgaagt aggcgtagac cgaccaggcg cccagcagcg
     9121 agaagcgggg tagatcgcgc accttccgcc cggtccacaa ggcccaggcc acggccgcga
     9181 ccgtcagagc cgtcgccgca aggcgcgggt ccgggtaccc cagctccgcg aggcgggtta
     9241 tcgccagcgg ccgacggacc ggcgacagga aggcgccggc cacgcccatt tcggcggcgg
     9301 cgtaggccgc ccgcatgatg taggtcacga tccaccagag gttggcggcc tggccggaca
     9361 gcatgtcgtg ccggccgagc gagcgcatcg cctgcacgaa gttcggccac gcgcccgccg
     9421 cggcaatggg cgccaggatc gcggctgccg agaggatcgc cgcgcctgcg gcgaggctga
     9481 gggatctcgc cctgggccgg gacgcgcctc cggacgggga gacggcgagc gccagggcga
     9541 cggccggcgc gaacaggacg gcctgcggct tggtcaggat ggcggcagcc atcagcgcgc
     9601 ccgacaggcc cgccgctccc gacgcagcag cgaccagggc tccgagggcg ggcagggcga
     9661 acagcgcgtc gaggtatccg agcaccggtg tcgcgaggat cgaggccgga ttgagccaga
     9721 aagccagcgc cgcgaaccgg gcccgatcgg cccgatgagg ctgcacgcgc cgcaccgcgg
     9781 cccacaacac gagggtcatc gcgccttcgc atgccagggg cagggccttg atgcatgcgg
     9841 cgagcagcgg cgtgtttgcg aagcccgggc gcgcccagcc gtagaggcgg cccgccgcgg
     9901 agagcgcgta cagggccacc ggagggtagt cgaccgtcgc ctcccagtgg tcgtactcgt
     9961 ggacccggcg ctccggcggc gtgccgccga cgccgtacat ctccgcgacg gggcccgtgg
    10021 ctgccgcgtg cgcccagacc ttgaacgcgg tgacgtcgga cgtgcccggc agaggaagcg
    10081 cggcaagacg gacggccaga ccggccagca ccgcgaccgc gaagaatcgg cccgatcgca
    10141 cgcgcgcagc ctcctagtgc acccagccgc cgccgccgtt cccagggcgg cccccgcggc
    10201 cggatcgtcc gccgtcgcga acgtcgaacc gccctctcag gcgggccatg cgccaccgca
    10261 gccactgcga gcgcagcgcc gatccgagcc cgccgcggcg ccaggcgagg tagaggtacc
    10321 cgaccacgag gccgccgagg tgcgtggcgt gggccacgcc gccgcgcggg tccgagacgg
    10381 aggacagaaa cgagatggcg ccgacaatca gcacgaagac gcgcgcccgg atcgggaaca
    10441 ggaagtacag gtagatcggg cggtcggggt acatcatcgc gtagccgacc aggaggccgt
    10501 aaatggcgcc ggacgcgccg acggtcaccg atgcgtagat tgcctgtgag aacccgaagg
    10561 gcagcagcga caccccgatc gtcgtcagcg ccgcccccgc gccagtgacg gcgtagtagc
    10621 gcacgaaaaa gcgcgtgccc catcgccgtt cgagatcgac gccgaacatc cacagggcca
    10681 gcaggttgaa cacgaggtgg aacagccagc cgtgctggaa gaggtaggtg agcggctgcc
    10741 agaaccatcc ccgctccagg accagcgcgg gcacgagccc cagccgaatg acgagaccgg
    10801 gaaccgccag cgtgatcagg tacccggccg tggtcacgac gagcagcgcg cgcacggccg
    10861 gcgtcatcgg cccgggaccg gacgcgtacg agaggcgcga cggacggtac tgcatctcag
    10921 acccttccca gtgcccgttc atcgggccgg cgcacggcgg attccgtttt cgccccggac
    10981 ggcaggggca tggagcaggc cgccgcaagg agcggcagga acgccacggc ggtcagcgtc
    11041 gtgccgatgc cgatgcggtc ggcggcggcc ccgacgatcg gcgccatgag tccgcccgtg
    11101 ccccacgcga accccagcat cagtgacgag accgtggccg cgccggtcgg cgcgatctgg
    11161 tgcgcgaagg tcacgttcac gggcagcgtc gactggagga agaaccctcc gagggccagc
    11221 accgccacca ggggccagtc ggtcaaccgc gtcgccgccg cgagcagggg agcggccagg
    11281 accagcgacc cggcaatcac ccgcttcggg cccagccgat cggccagcgg tccgccggcg
    11341 aatcccccga gcccgccggc cgcgaggtag accgacacgg ccacgccggc catcccgacc
    11401 gaccatccgc gcgtcgtcag cagcacgggc acgaacgtcg cgatgctcag cgacgtcatg
    11461 gtcctcagga cgacgatgag ccacagcagg aagagcggct tggcgaaggg ccggagcgcc
    11521 aggtacccgc cgccgcttcc gctcgccagc gggcgcatca gcggcatgcc cctcaggacg
    11581 agggccagga gggcgaggcc gggaagcgcc agcaggggcg tagaggtcag gccgaagcgg
    11641 cccacgtaag gggcgaacag gagcggcccg accgcgtagc caatcgagcc gcccgtgatg
    11701 tggatggcca tggccaggcc gcgccggctc ccgccctggc gatggacgac cgccgccgcc
    11761 gtcggatgga acgccgcacc gccgagcccg ccgatgacga ggacgccggc gagcgtcgcc
    11821 ggggtccacg ccatgccgac gaggctcagc accgtgaccg aaacgatcgg ccccgcgacg
    11881 aggaaggcgt gcggcctcca gcgg
//
LOCUS       DAOSIF010000033        11845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5412_length_11845_cov_4.156064, whole genome shotgun sequence.
ACCESSION   DAOSIF010000033 DAOSIF010000000
VERSION     DAOSIF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5412_length_11845_cov_4.156064"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLT35_02260"
     CDS             complement(<1..313)
                     /locus_tag="PLT35_02260"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOG28079.1"
                     /translation="MKKLVLIRHGESTWNKENRFTGWTDVDLSDKGVQEAIDGGRLLR
                     EGGYVFDVAYTSVLKRAIKTLWIALEQLDQMWIPVHNTWRLNERHYGALQGLNKAETA
                     AK"
     gene            complement(467..1549)
                     /locus_tag="PLT35_02265"
     CDS             complement(467..1549)
                     /locus_tag="PLT35_02265"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOG28080.1"
                     /translation="MAGLRDALDGALLLAPMTRGGNLPYRRLCVELGARVLMGEMTVA
                     RRLKQRRRGEYALIRRAPDEPFFGVQLAGTDADEMGWAAALAEERGADLVDINFGCPI
                     DVFTRRGLGAALGRQPKRIRRIVEGVKRAVVRIPVTAKIRLGWNESSRNYLEQAQAAV
                     DGGADALVVHGRTRDARYRQAADWDAIGELVAALPVPVVGNGDALFPADIEAARARSG
                     CAAVMAGRGALIKPWLFREAREGYWDLSAEDRLRIYRRYVDLAREHWGEDAHGLARVG
                     EFVRWHLAFWCRYAPRRADGTWPGMQVREPMSFARSPLEALLARADHATLDWLARRLT
                     AREEIVPTEAPAPDSAPDPQDDQAEG"
     gene            complement(1556..3214)
                     /locus_tag="PLT35_02270"
     CDS             complement(1556..3214)
                     /locus_tag="PLT35_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOG28081.1"
                     /translation="MSNCRRLLARASALLVGLAVLPAVPASARQQDLAALTAPQAYED
                     PLRRDTPRGAFMGFVDAIQRDDLALAADYLQWPRQRMPIGREEAANQLRFVLNHGFEG
                     NLDRLSRDPAGAANDGLPADRERVGTAVLASGERVEIYLARVPQDSGIPIWLVASDTV
                     SDIPRMYKNAGLPQLERRLPKVLTRTDFGRLQLWVPLALLLLLPALYVVSAVILWLVL
                     EAVRLVLRLRGRPIQGRRSPIVRALARPTVFILTVLLHRLLSPHIGIPLLHRQYYSRT
                     VTVVLLLTAVWWVWRLIDLAAGRTREGIQPDSPRAAQRVYSLSRRLLKGVVAFVALMV
                     GLSAFGVNLTTALAGLGIGGLALAFGAQKTIENVFGGIFVLSDRSIVVGDFCRIGNYT
                     GTVEDVGLRSMQLRTLERTVVSVPNGTLATMEVENFGRRDKFLFNPTIALRYETTLPQ
                     LRRVLADIRAVLAAEASVEASTARARFVRLGAYSFDIEILAYVRAKDYAAFLAEQEGL
                     LLRVIEAIKAAGTDIAFPSQTMYMKPAAALPASAAPPGAADDGK"
     gene            complement(3478..4836)
                     /locus_tag="PLT35_02275"
     CDS             complement(3478..4836)
                     /locus_tag="PLT35_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOG28082.1"
                     /translation="MARRRLAGVLRGAAVAAVLAHAWLAPAPAAEAAPIRPAAWAAAS
                     GASGADPVALDEQGGPGEVIAEVRIRGNYRTPDEEVLRIAGVSIGTPLPAGGPEAVAE
                     RLRRSGRFAAAEVRKRYRSLTDESQVALVIVVAERPGVERGGVMPGPMKRLRNALMFS
                     PTADYADGYGVTAGGRFTVAGALGTRGHVSVPLSIGSTRRAALELDKTPGSGPVRRLR
                     AGAAAASRENPAYGLRDRRGEVSAEASGSIGRLLGVAARAAWADISFGEVRDRAVSYG
                     ASLTFDTRANPAFPRNAVFLSAAWIVVDPASGPAVNRCALDGRAYAGLLGSSVLALEV
                     RSETTDRPLPVYERRLLGGASSLRGFRAGAFTGDNLASASLELRVPFHSPMRMAQSGI
                     VLFGDAGAAYEHGIRLRDAGRHYGAGAGVYVRAPLLQLEIDVAHGFGRGTRLHVTAGF
                     SF"
     gene            complement(4838..5995)
                     /locus_tag="PLT35_02280"
     CDS             complement(4838..5995)
                     /locus_tag="PLT35_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28083.1"
                     /translation="MGPTPRRPTGRAGFAALLACLAIGAASGGPLLRARQPAPPSPRL
                     SDLDAFMARVLERRNDNWKTLHDYILSERERFQVLGPGSVPLVGQRRDFIWFIRDGFL
                     VRSPITANGVAIAETDRREYEAAWLAGEQAREKRAREKREGRAGAAEAAAGAAGSAAD
                     AAIVEGLGGIEPLPEREAASMVGGEPRFISEAYFMKFRFEPGNYYLAGRETLDGRPVV
                     KVEYYPSRMFDGEDKERREKTEVRIDAASGEVSVRPAEEKPAPAKRRAAGQAPEDDDL
                     DREIEAAMNKVTQVTMWIDPQEFQIVKYTIDNADWGFLPGRSIVRVDEARASMTMGRY
                     FDGVWLPNAIVFRFGATFAAGSYTLLYTREFHDYRRGEVSARIRAYVPREP"
     gene            complement(6058..9126)
                     /locus_tag="PLT35_02285"
     CDS             complement(6058..9126)
                     /locus_tag="PLT35_02285"
                     /inference="COORDINATES: protein motif:HMM:NF004799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG28084.1"
                     /translation="MPTLTHDQPSFALDAAERAARHLYGLEAGAEPLPGERDQNVLLT
                     TPSGERFVMKISSASEDLQALDLQNQVLARLAAAAPSLALPRVRFAPDGAAIARLLGP
                     DGEAHPVRLLTWVRGRVLAGVRPHTPALLGSLGALLGALDAALLGFDHPAADRDLGWD
                     PRHTAWLREYLPIVEGPDRRSLLARLLDAAEATIARLSAHLPVGIVHNDANDYNVIVD
                     GRDPYTRRVSAVVDFGDTQRTFLANEVAVAAAYAALGKPDPLAAAAPIVEGYHAARPL
                     GEAEMEAIFPLVCLRLCVSVVNSAYQRRADPANRYLTVSEGPAWELLGRLEAIHPRFA
                     HYVFRRACGLEPCPSAPAVRAWLRSRARHIGPLLDPDARAAPRTVCDLGIGSPEAGTP
                     DVWADPDRFSRDIASRMAAAGARVGVGRYDEVRGVCASSLSAKPGNDGPTWRTVHLGL
                     DLFVDPGTPVLAPLPGVVRAAADNAGPLDCGPTVVLEHEAGAGGPVFYSLYGRLARAS
                     MARLAPGARVAAGDVIARVGAREENGGWPPHLHFQLITDMLDRQADFPGAADPAERDV
                     WLSLSPDPNLAALLPEGAAALRPRPAGEILARRRDSIGPSLSVAYRRPLAVVRGWMQH
                     LYDADGRAYLDAVNNVPHVGHCHPRVAETGRRQMALLNTNTRYLHERLVEYASRLSAT
                     LPDPLRICYFVNSGSEATELALRLARAFTGSRETIVVDVAYHGNTGAAIEISPYKFNG
                     PGGSGAPPHVHVAPAPDLYRGLYRDAAADAGARYAAHVADAVDRILRAGGRPGAFIAE
                     SVLSCAGQIVLPPGYLDAAYGHVRAAGGVCIADEVQTGFGRVGSRFWAFETQGVVPDI
                     VTMGKPMGNGHPLGAVVTTRAIADAFAGGMEYFNTFGGNPVSCAIGLAVLDVIRDEGL
                     QQRAERSGARLLDRLRELMSRHPLIGDVRGLGLFAGIELVRDRDTREPAAAEAGLVAN
                     RMRDRGVLVSTDGPFHNVLKIKPPLCFAEPDADRLASELDEILGEDYFQA"
     gene            9277..9936
                     /locus_tag="PLT35_02290"
     CDS             9277..9936
                     /locus_tag="PLT35_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28085.1"
                     /translation="MTRRSVRTARAVLAAAMLAAPAPLLAQDGPVAFTLGGAVVGPLS
                     DSADRFTTGPGIAAGAWWNLTEQSIVRIDGVWSRLGSSDDWPAPPLDVQARANARFQF
                     ASAAFMFQGPPARVRAYILAGFGLYRRTVRVSASGGPSAGVCDPYWFVCESGPVSAGR
                     LAGSRSSTDLGLNVGAGVRAGRCFAEIRYHAMWGPRFETPDGTARATGRFLPLTVGVM
                     F"
     gene            10003..10737
                     /locus_tag="PLT35_02295"
     CDS             10003..10737
                     /locus_tag="PLT35_02295"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOG28086.1"
                     /translation="MRQSVVAIALAALCVPLPASAEPADPPPPPRHEGTAELSYVGTS
                     GNSSTQTIGLGGEFICRPDQWVITNKAAFVRNKSESELTAETYVYLFKAARTISSRLA
                     GFGQYDYFRDEFAGMLHRHSLLGGVQYKIVELSEHLVTADGGLGYVNEQRIAGADLSL
                     ASWSAGAAYRWKISETAEFTDDVQLLGLFSERGGWRLKQSASVTARLTELLSLKVANT
                     IRYVSQPVPGFKSTDTMTSIALVAKF"
     gene            10756..11541
                     /locus_tag="PLT35_02300"
     CDS             10756..11541
                     /locus_tag="PLT35_02300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG28087.1"
                     /translation="MEGWRGWDDYARFYDWENARTMGRRDIGFWQQLAGRAGGPVLEL
                     GCGTGRVSIPMAKAGARVVGLDRSAAMLARARTRARRSKLAGSLCLVRGDVRALPFAA
                     AAFPLVAAPYGILQSLLDDATLDAAVASAHRVLAPGGTFAIDLVSDLASWDEYERRVK
                     LRGPAGRSGARITLIESVRQERRRGVTAFDQEFVERRGAHVRSHRFSLAFRTVSVEGM
                     AARLERHGFRVAAVLGDYDGRPWDPRADVWLLLAVRERARLPG"
BASE COUNT         1601 a         4212 c         4395 g         1637 t
ORIGIN      
        1 gcttggcggc cgtctcggcc ttgttcagcc cctgcagcgc gccgtagtgc cgctcgttca
       61 ggcgccacgt gttgtgaacc gggatccaca tctggtccag ctgttcgagg gcgatccaca
      121 gcgtcttgat cgcccgcttc aggaccgacg tgtacgccac gtcgaacacg tagccgcctt
      181 ccctcagcag ccgcccgccg tcgatggcct cctggacgcc cttgtcggac agatcaacgt
      241 cggtccagcc cgtgaaccgg ttctccttgt tccaggtgct ttctccgtgc cggatgagga
      301 ccagcttctt cataggattc gtcacgaccc cctgcctaga tcctcccccg aaaacgggct
      361 ctggcacaat cgcccgcccg cggcggcagg tgccacgggg gccgcgtttc ttccggcgag
      421 gcgacgcaca ggacggggcc gggccccctg gccgggggga aggcggctac ccttcggcct
      481 gatcgtcctg cgggtcggga gccgagtccg gtgccggggc ctccgtcggg acgatctcct
      541 cccgcgccgt caggcgacgg gccagccagt cgagcgtcgc gtggtcagcc cgggcgagca
      601 aggcctcgag cggcgagcgg gcgaagctca tcggctcgcg gacctgcatg ccgggccacg
      661 tgccgtcggc tcgccggggc gcgtagcggc accagaacgc cagatgccag cggacgaact
      721 cgccgacgcg cgccaggccg tgagcgtcct cgccccagtg ctcccgggcc agatcgacgt
      781 acctcctgta gatgcgcagc cggtcctcgg cgctgagatc ccagtaccct tcgcgggctt
      841 cgcgaaacag ccagggcttg atgagggcgc cgcggccggc catgaccgcc gcgcagccgg
      901 atcgcgcacg ggccgcctcg atgtccgccg ggaagagcgc gtcgccgttc ccgaccaccg
      961 gcacgggcag cgccgcgacg agctcgccga tggcgtccca gtcggccgcc tgccggtagc
     1021 gcgcatcgcg cgtcctgccg tggacgacga gcgcgtctgc gccgccgtcc acggcggcct
     1081 gcgcctgctc gaggtagttg cggctggact cgttccagcc cagccggatc ttcgccgtca
     1141 cgggaatgcg aaccacggcg cgcttcacgc cctcgacgat ccggcggatg cgcttcggct
     1201 ggcgtccgag cgcggccccc agcccccggc gcgtgaagac gtcaatcggg cacccgaagt
     1261 tgatgtccac caggtcggcc ccgcgctcct ctgccagggc cgcggcccac cccatttcgt
     1321 ccgcatcggt cccggcgagc tggacgccga agaacggctc atcaggcgcg cgcctgatca
     1381 aggcgtactc gccgcggcgc ctctgcttca agcgcctcgc gacggtcatc tcgcccatga
     1441 gcacccgcgc gcccaactcg acgcacagcc ggcggtaggg caagttcccg cccctagtca
     1501 tcggcgccag caggagggct ccgtcgagcg cgtcgcgcaa gccggccatg cgcggctact
     1561 tcccatcgtc ggcggcgcct ggaggcgccg ccgacgccgg cagcgcggcg gcaggcttca
     1621 tgtacatcgt ctgcgacggg aacgcgatgt cggtgccagc cgccttgatc gcctcgatga
     1681 cgcgcagcag cagcccttcc tgctcggcga ggaacgcggc gtagtccttg gcccgcacgt
     1741 aggcgaggat ctcgatgtcg aaggagtacg cgcccaggcg gacgaaccgc gcgcgggccg
     1801 tcgaggcctc cacgctggct tccgcggcca gcaccgcacg gatgtcggcc aggacgcgcc
     1861 gcagctgcgg cagcgtcgtt tcgtaccgga gggcgatcgt cgggttgaac aggaacttgt
     1921 cgcgcctccc gaagttctcg acctccatgg tcgccagcgt gccgttcggg acggacacga
     1981 ccgtgcgttc gagcgtgcga agctgcatgg agcgcagccc cacgtcctcc accgtccccg
     2041 tgtagttccc gatccggcag aagtcgccga cgacgatgct gcggtcgctc aggacgaaga
     2101 tgccgccgaa gacgttctcg atcgtcttct gcgccccgaa ggccagagcg agcccgccga
     2161 tcccgaggcc ggcaagggcc gtcgtcagat tgacgccgaa ggcgctcagc ccgaccatga
     2221 gggcgacgaa ggccacgacg cccttcagga gccggcggct cagcgagtac acccgctggg
     2281 ccgctcgcgg gctgtccggc tggattccct cgcgcgtgcg gccggcggcc agatcgatga
     2341 gccgccagac ccaccagacg gccgtgagca gcaggaccac ggtgacggtg cgggagtagt
     2401 actggcggtg cagcagcggg atcccgatgt ggggcgacag cagccgatgc agcaggacgg
     2461 tgaggatgaa cacggtcggc cgggccagcg ccctcacgat gggcgagcgg cgcccctgga
     2521 tcgggcggcc gcgcagccgc agcacgagac gcacggcctc cagcaccagc cagagaatga
     2581 cggctgacac gacgtacagc gccggcagca gcagcaggag cgccaggggc acccagagct
     2641 gcagccgccc gaagtccgtc ctggtcagaa ccttcgggag cctccgctcg agctggggca
     2701 ggccggcgtt cttgtacatc cgcggaatgt cgctcaccgt gtcggaggcg acgagccaga
     2761 tcgggatccc cgagtcctga ggcacgcgcg cgaggtagat ctcgacgcgc tcgccgcttg
     2821 cgagcaccgc cgtgccgacg cgttcccggt cggcgggaag cccgtcgttc gccgcgccgg
     2881 cgggatcccg gctcagccgg tcgaggttgc cctcgaagcc gtggttcagc acgaaccgga
     2941 gctggttggc cgcttcctcc ctgccaatgg gcatccgctg ccggggccac tggaggtagt
     3001 cggcagccag cgcgaggtcg tcgcgctgga tggcgtcgac gaatcccatg aaggcgcctc
     3061 tcggcgtgtc gcgccgaagc gggtcttcgt acgcctgggg ggcggtcagc gcggcgaggt
     3121 cctgctggcg ggcggaggct gggaccgccg gcagcacggc caggccgacg agcagggcag
     3181 acgctctcgc gagcagtcga cggcagttcg acatgaatcg gctccctcgt tcattctagc
     3241 ccggacgacc cggggccctc ggtcccggcg ccagcgcggg gcgcgcgccc atccggcgct
     3301 tcgcgctccc tggcccgatt ggctgcgtcg gcgcggatcc ggccgcccgc ctcagcctag
     3361 cccgcgccgg gaacccggta tccgtcgccc ggaacggctg ccggagtgac ggcatcgccg
     3421 tctcgatggg cacggcgttc ccggtcgcct gaggcgtggg gatggaagag gccgggctca
     3481 gaacgagaag ccggcggtga catgcaggcg cgtgccgcgt ccgaagccgt gcgccacgtc
     3541 gatctcgagc tgcaggagcg gcgctcgcac gtacacgccc gctccggccc cgtagtgccg
     3601 gccggcatct cgaagccgga ttccgtgctc gtaggcggcg cccgcgtccc cgaagagcac
     3661 gatgccggac tgcgccatcc gcatcggtga atgaaacggc accctcagtt cgagggaggc
     3721 cgaggcgagg ttgtctccgg tgaaggcgcc ggccctgaag ccgcgcagcg aggaggcgcc
     3781 gccgagcagg cgccgttcgt acacgggcag cgggcggtcc gtcgtctccg acctcacttc
     3841 gagcgccagc acggaggatc ccaggaggcc ggcatacgcg cggccgtcga gggcgcagcg
     3901 gttgacggcc gggcccgagg ccgggtcgac gacgatccac gcggccgaca ggaagacggc
     3961 gttccggggg aaggcgggat tggcgcgcgt gtcgaacgtc aggctcgcgc cgtacgagac
     4021 ggcacggtcg cggacttcgc cgaacgagat gtccgcccag gccgcccggg cggcgacacc
     4081 gaggagccgc ccgatcgagc cgcttgcctc cgccgacacc tcccctcgac ggtcccgcag
     4141 gccgtacgcc gggttctcgc ggctggcggc ggcggcgccg gcgcgaaggc gccggacggg
     4201 gcccgaaccc ggcgtcttgt cgagctcgag cgcggcgcgc cgggtcgagc cgatgctgag
     4261 aggcacggag acgtggccgc gcgtgcccag ggcgccggcg acggtgaacc ggccgcccgc
     4321 cgtgaccccg taaccgtcgg cgtagtccgc cgtcggcgag aacatcaagg cgttccggag
     4381 ccgcttcatc gggccgggca tcacgccgcc gcgctcgacg cccggccgct cggccacgac
     4441 aatgacaagc gccacctgcg actcatccgt cagcgagcgg taccgcttcc ggacctcggc
     4501 cgcggcgaac cgtccgctgc ggcgcagacg ctcggcgacg gcttccgggc cgccggccgg
     4561 aagcggcgtc ccaatcgaga cgccggcgat ccggagcacc tcctcgtccg gcgtgcggta
     4621 gttgccgcgg atccgcacct cggcgatcac ctcgccgggg ccgccctgtt catcgagggc
     4681 gacaggatcg gccccgctcg cgccggatgc ggcggcccac gcggccgggc ggatgggcgc
     4741 cgcctcggcc gcgggtgcgg gcgcgagcca ggcatgggcc aggaccgcgg cgacggcggc
     4801 cccgcgcagc acgccggcga ggcggcggcg agccacgcta gggctccctc ggcacgtagg
     4861 cccggatcct cgcgctgacc tcgcccctgc ggtagtcgtg gaactcccgg gtgtagagga
     4921 gcgtatacga gccggccgcg aaggtggcgc cgaacctgaa cacgatcgcg tttggcagcc
     4981 acacgccgtc gaagtaccgc cccatcgtca tggaggcgcg ggcctcgtcc acgcggacga
     5041 tcgagcggcc ggggaggaag ccccagtccg cgttgtcgat cgtgtacttg acgatctgga
     5101 actcctgcgg gtcgatccac atggtgacct gggtcacctt gttcatcgcc gcttcgatct
     5161 cgcgatccag atcgtcgtct tcgggggcct ggcccgccgc gcgccgcttc gcgggcgccg
     5221 gcttctcctc cgccggccgc acgctcacct cgccggaggc ggcgtcgatg cggacctcgg
     5281 tcttctcgcg ccgctccttg tcctcgccgt cgaacatgcg cgacgggtag tactcgacct
     5341 tgacgacggg gcggccgtcg agcgtttcgc gcccggcgag gtagtagttg ccgggctcga
     5401 accggaactt catgaagtac gcctccgaga tgaagcgggg ctcgccgccg accatgctgg
     5461 cggcctcccg ttcgggcagc ggctcgatgc cgcccagccc ctcgacgatg gcggcgtcgg
     5521 cggcgcttcc tgctgctccc gcggccgcct ccgcggcgcc ggcgcggccc tcccgcttct
     5581 cccgcgcccg cttctcccgc gcctgttcgc ctgccagcca cgcggcctcg tactcgcgcc
     5641 gatccgtctc ggcgatggcg acgccgttcg ccgtgattgg gcttcgcacg aggaagccgt
     5701 cgcggatgaa ccagatgaag tcgcggcgct ggccgacgag cgggacgctg cccgggccca
     5761 gcacctggaa gcgctcgcgc tcgctcagga tgtagtcgtg gagcgtcttc cagttgtcgt
     5821 tgcggcgctc gagcacgcgc gccatgaagg cgtcgaggtc ggacaggcgc ggcgacggcg
     5881 gcgccggctg ccgggcccgc agcaacgggc cgccggaggc cgccccgatg gcgaggcagg
     5941 cgagcagggc cgcgaacccg gcgcggcccg tcggtcgacg cggcgttggc cccatgtcct
     6001 gcttgtgacg agcgcggccg gcgcgaagtt cgccggcccc ggctgttcgc tgccgcctca
     6061 ggcctggaag tagtcctctc cgaggatctc gtccagctcg gaggcgaggc ggtctgcgtc
     6121 cggttcggcg aagcagagcg ggggcttgat cttgaggacg ttgtggaacg gcccgtccgt
     6181 gctgacgagc acgccgcggt cgcgcatacg gttcgcgacg agcccagcct cggccgcggc
     6241 gggctcccgc gtgtcccggt cgcgcacgag ctcgatgccc gcaaacaggc ccaggccgcg
     6301 cacatcgccg atgagcgggt ggcggctcat gagttcccgg aggcgatcga ggaggcgggc
     6361 gccgctgcgt tcggcccgct gctggagtcc ttcgtcgcga atgacgtcga gcacggcgag
     6421 gccgatggcg cacgagacgg ggttgccccc gaacgtgttg aagtactcca tgccgccggc
     6481 gaacgcgtcc gcgatcgccc gggtcgtcac cacggccccc agcgggtgcc cgttgcccat
     6541 gggcttgccc atcgtgacga tgtccggcac gactccctgg gtctcgaacg cccagaaccg
     6601 ggagccgacg cgcccgaatc cggtctgcac ttcgtcggcg atgcagacgc cgccggcggc
     6661 gcgcacgtgg ccgtacgccg cgtcgaggta gccgggcggg aggacgatct ggccggcgca
     6721 gctcaggacg gattcggcga tgaaggcgcc cggccggccg cccgcgcgca ggatgcggtc
     6781 gacggcgtcg gcgacgtgcg cggcgtagcg cgcccccgcg tccgcggcgg cgtcgcggta
     6841 cagcccgcgg tacaggtcgg gcgcgggcgc cacgtgcacg tgcggcggcg cgcccgaccc
     6901 gccggggccg ttgaacttgt acgggctgat ctcgatggcg gccccggtgt tgccgtggta
     6961 ggcgacgtcc acgacaatcg tctcgcggct ccccgtgaag gcgcgggcga ggcgcagcgc
     7021 gagttccgtg gcctcgctgc cggagttcac gaagtagcag atgcgcagcg gatcgggcag
     7081 cgtcgccgac aggcggctgg cgtactccac gaggcgctcg tgcaggtacc gcgtgtttgt
     7141 gttgagcagc gccatctgac gccggcccgt ctcggcgacg cgcgggtggc agtggccgac
     7201 gtgcggcacg ttgttgaccg cgtcgagata ggcccgcccg tccgcgtcgt agaggtgctg
     7261 catccagccg cgcacgacgg cgagcggacg ccggtaagcg acgctgaggg acgggccgat
     7321 cgagtcgcgc cgccgggcga ggatctcgcc ggcggggcgg gggcgcagcg cggccgcgcc
     7381 ttccgggagg agggcggcca ggttcgggtc gggcgacagg ctcagccaca cgtcgcgctc
     7441 ggctggatcg gccgcgcccg ggaagtcggc ctgccgatcc agcatgtcgg tgatcagctg
     7501 gaagtgcagg tgcgggggcc agccgccgtt ctcctcccgc gcgccgacgc gcgcgatgac
     7561 gtcgccggcc gccacacggg cgcccggggc aagcctcgcc atcgacgcgc gcgcgagacg
     7621 gccgtaaagc gagtagaaga cggggccgcc cgcgccggct tcgtgctcca gcacgacggt
     7681 cggaccgcag tcgagcgggc cggcgttgtc ggccgccgcg cggaccacgc cggggagcgg
     7741 ggcgaggacc ggcgtgccgg gatccacgaa caaatcgagg ccgaggtgga ccgtgcgcca
     7801 ggtggggccg tcgttgcccg gcttcgcaga caggctgctc gcacagacgc cgcgaacctc
     7861 gtcgtagcgg ccgacgccga cgcgcgcgcc tgctgccgcc atccgcgacg cgatgtcgcg
     7921 cgagaaccgg tcgggatcgg cccagacgtc cggcgtgccc gcctcagggc tgccgatgcc
     7981 gagatcgcag accgtgcggg gcgcggcgcg cgcgtccgga tcgagcagcg gcccgatgtg
     8041 ccgggcgcgg gaccgcagcc acgcccggac cgccggcgcc gaggggcatg gctcgaggcc
     8101 gcacgcgcgc cggaacacgt agtgcgcgaa gcgcgggtgg atcgcctcca gccgaccgag
     8161 cagctcccac gcggggccct cgctgactgt gagataccgg ttcgccgggt ccgcccggcg
     8221 ctggtacgcc gagttgacga cgctcacgca cagccggagg cagacgagcg ggaagatcgc
     8281 ctccatctcg gcctcgccga gcgggcgcgc cgcgtgatag ccctcgacga tcggcgcggc
     8341 ggccgcgagc ggatccggct tgccgagggc cgcgtacgcc gccgccaccg ccacctcgtt
     8401 ggcgagaaac gtgcgctgcg tgtcgccgaa gtccacgacg gcggagacgc ggcgcgtgta
     8461 cggatccctc ccgtcgacga tgacgttgta gtcgttggcg tcgttgtgga cgatcccgac
     8521 gggcaaatgc gccgagaggc gtgcgatggt ggcttccgcg gcgtcaagca gccgcgcgag
     8581 cagcgaccgg cgatcggggc cctcgacaat cggcaggtac tcgcgcagcc aggccgtgtg
     8641 ccgaggatcc cagccgagat cccggtccgc tgcggggtga tcgaagccca ggagggccgc
     8701 gtcgagggcg ccgagcaggg cgcccaggct gccgaggagg gccggcgtgt gcgggcgcac
     8761 gccggcgagc acgcggccgc ggacccacgt cagcaggcgc accgggtgcg cctcgccgtc
     8821 cgggccgagg agccgggcga tcgcagcgcc gtcgggcgcg aaccgcacgc gcggcagcgc
     8881 gagcgaaggg gccgcggcgg ccagacgcgc cagcacctgg ttctggagat cgagcgcctg
     8941 caggtcttcg gaggcgctcg agatcttcat gacgaaccgc tcgccgctcg gcgtcgtcag
     9001 caggacgttc tggtcccgtt cgcccggaag cggttccgcc cccgcctcga gcccgtagag
     9061 gtgccgcgcc gcccgctcgg ctgcgtcgag ggcgaacgac ggctggtcgt gcgtgagcgt
     9121 gggcatcggc gcggcgccgg caccaggggc caggcggcta cggcattcta cccgcatcgc
     9181 gccgatccgc cggatgggca cggaggcgga gcgcccgtcc ggcgaggcga atgacgcgtg
     9241 gtgtagactg gcgttttggg acggtcgatg gcggtcatga cgaggaggag cgtccggacg
     9301 gcgcgggccg tgctggcggc ggcgatgctg gccgcgccgg cgcctttgct cgcccaggac
     9361 gggccggtcg cgttcacgct cggcggcgcc gtcgtggggc cgctgtcgga cagcgcggat
     9421 cgcttcacga cgggcccggg catcgctgcc ggcgcgtggt ggaacctgac cgagcagtcc
     9481 atcgtgagga tcgacggcgt ctggtcgcgg ctcggctcga gcgacgactg gccggcaccg
     9541 ccgctcgacg tgcaggcgag ggcgaacgcc cgcttccagt tcgcgtcggc cgccttcatg
     9601 ttccaggggc ctccggcgcg ggtgcgcgcc tacatcctcg ccggcttcgg cctctaccgg
     9661 cgcaccgtgc gggtctcggc ctcgggaggg ccatcggccg gcgtgtgcga cccgtactgg
     9721 ttcgtgtgcg agagcgggcc cgtgagcgcg ggccggctgg cgggatcgcg atccagtacc
     9781 gacctcgggc tgaacgtcgg cgccggcgtc agggccggcc ggtgcttcgc cgagattcgc
     9841 taccatgcca tgtgggggcc gcggttcgaa acgccggacg gaacggcgcg ggcgacgggc
     9901 cggttcctgc cgctgaccgt cggtgtcatg ttctgagcgc ggcgcccgct ggtctggccc
     9961 cgttccgcgg gcgggccgcc ctttcgctga ggagtcgatg aagtgaggca atccgttgtc
    10021 gcgatcgcgc tggccgcgct gtgtgtcccc ctcccggcgt cggccgagcc ggcagacccg
    10081 ccgccgccgc cgaggcacga gggcaccgcg gagctctcgt acgttggcac ctccggcaac
    10141 tcctcgacgc agaccatcgg actcggcggg gagttcatct gccggccgga ccagtgggtc
    10201 atcaccaaca aggcggcctt cgtgcgcaac aagtccgagt cggagctgac ggccgagacc
    10261 tacgtgtacc tcttcaaggc ggcgcggacg atctcctccc ggctggcggg ctttggccag
    10321 tacgactact tccgcgacga gttcgcgggc atgctccacc ggcactcgct gctcggcggc
    10381 gtccagtaca agatcgtcga gctctccgag cacctggtga ccgcggacgg cgggctgggc
    10441 tacgtgaacg agcagcggat cgccggggcg gacctgtcgc tggcgtcctg gtcggctggc
    10501 gccgcgtatc gctggaagat ctccgagacg gccgagttca ccgacgatgt gcagctcctc
    10561 gggctgttct cggaacgggg cggctggcgc ctcaagcagt ccgcctcggt gacggcgcgc
    10621 ctgaccgagc tgctctcgct gaaggtggcg aacacgatcc gctacgtcag ccagccggtg
    10681 ccggggttca agagcaccga cacgatgacg tcgatcgcgc tcgtcgcgaa gttctgagcg
    10741 ccggcgggct cgcacatgga aggctggcgc ggctgggacg actacgcccg gttctacgac
    10801 tgggagaacg cccggacgat gggccggcgc gacatcgggt tctggcagca gctggccggt
    10861 cgggcgggcg ggcccgtgct cgaactcggg tgcgggaccg gtcgggtctc gattccgatg
    10921 gcgaaggccg gcgcacgggt ggtcggcctg gaccggtcgg ctgcgatgct tgcgcgggcc
    10981 cgcacgcggg cgaggcggtc gaagctggcc gggtcgctct gcctggttcg cggcgatgtg
    11041 cgcgccctgc cgttcgcggc ggccgccttc ccgctggtgg ccgctccgta cggcatcctc
    11101 cagtcgctgc tcgatgacgc caccctcgac gccgctgtgg cgtcggcgca ccgcgtcctg
    11161 gcgcccggcg gcacgttcgc gatcgatctc gtgtcggatc tggcgtcctg ggacgagtac
    11221 gagcggcggg tgaagctcag ggggccggcc gggcggagcg gggcgaggat caccctgatc
    11281 gagtcggtgc gccaggagcg ccgccggggt gtcacggcgt tcgatcagga attcgtcgag
    11341 cggcgggggg cgcacgtgcg ctcccaccgg ttctcgctgg ccttccggac ggtctcggtc
    11401 gagggcatgg cggcccgtct cgagcgccac gggttccggg tggcagcggt gctgggcgac
    11461 tatgacggcc gcccttggga tccgcgcgcg gacgtctggc tgctcctggc cgtgcgagag
    11521 cgggccaggc tcccgggcta ggattccgcg ccaggcgggc cgcacgggcc ccggttcccg
    11581 gatctccttc ccttgttggc ggtcggagcc gcatgttagg atcttgcggc gcatctccac
    11641 cgggtttccc gctgacactg ctggagttcc gatatgtccg gccattccaa gtggcacacg
    11701 atcaagcaca agaagggcgc cgccgacgcc aagcgcgggc gtctcttcac gcgcatcatc
    11761 aaggagctga ccgtggcggc ccgcgcgggc ggcggcgacc ccgattcgaa tccgcggctc
    11821 cgcacggtcg tggccgaggc caagc
//
LOCUS       DAOSIF010000034        11718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5474_length_11718_cov_4.163937, whole genome shotgun sequence.
ACCESSION   DAOSIF010000034 DAOSIF010000000
VERSION     DAOSIF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11718
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5474_length_11718_cov_4.163937"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1539)
                     /locus_tag="PLT35_02305"
     CDS             complement(160..1539)
                     /locus_tag="PLT35_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG28088.1"
                     /translation="MNTQTVVVGAGVAGLTIASRLAREGRRVTLVERDAWAGGLARTW
                     HYGDFHFDVGPHRFHTDNRRVEQFVREVLGSQAIEISRSSGARMFGRFHPWPLRPSVF
                     LSMPPSVMLRGAIDAVARRRVPGESFEADVLNKYGRTLYEIFFRPYTRKFLGSDPADL
                     HRDWGRAGVNRAVIDKRASADTIWSLIRTTLLPKPVETTFLYSPRGIGQFSDRLVSAI
                     RDCGGRVLLGRRVTALTLRDGRITAVAAGGEEIACDDVVWTGPITEAAALAGIGPVRL
                     EFLSTLFYNFEIGVPPRVPYQWTYFGGDEIFSRASSPTAFDRGMAPDGMSGLCVEVTC
                     REGDERWGGPEALVDGIVRDLARTGMIGSPDDVRGLHIEPVRNTYPIYGLDYPAELAS
                     CLRALGGVRNLLLAGRCGRFWYNNMDHSIGQGLTMADRILRGEALAGIDAGPRDFWEE
                     PPGSAPAGD"
     gene            complement(1536..3626)
                     /locus_tag="PLT35_02310"
     CDS             complement(1536..3626)
                     /locus_tag="PLT35_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28089.1"
                     /translation="MPDRTSTADRRGRLFLPDFLTAGRAAPVLIAAVGVWVLALAWRL
                     GSGWGGIVCLALVVASIAPGWPVAAYVFGARHGATWIAGFALGYPLTAFLLWIPIALG
                     VPRPETIVSTWAAGTAVIWAVLFPRRARRIDLPRWTRRDTAALAMVVALVPLLVGRPF
                     SRIGERDAAGERLFRAYFTADFVWHMALTAELEKADNPPRNPYRAKDTLHYYWLHFVP
                     PSSAALVMPHILPDRVGRLTVNALGTGMIFAAMVFLFAWMACGGRAGPSAVAVALAFV
                     AASAEGLYLIQRCLRGGAPLAAIREINVDATTYWFFQAVSIDGLQRSLYYNTHHSMAC
                     AVGLVALVIVARAGARMGGGVALACGASLGLALMVSPFPGGLISLTWGLAAAADLVID
                     RERFCSKVATLALAGLPVIGAVGWMVANQTLEGTGSNLRFGLSRLAMRAPLVAIPLAA
                     GPILAPALAGLAASWRLPRSARPALLAVVAAFLLIFFVTLTSAEPWIGWRAGQVMFVA
                     APGLGALGFAALWDRRRWAAIALAAGVALAGVPTTVLDYINAQDVETRTMGPGFPWTV
                     VVTRNEQEALDWIKRHTKPWQTVQVEPTARGRASWTLIPSFAERRMATGLPISLLDEP
                     AYHRASERVRELYATEDPDLAHRIARSMGIRYLYLGQVELEHYPEAGVKFDRRLDLFT
                     KVFQNPEVRIYSVQ"
     gene            3744..4268
                     /locus_tag="PLT35_02315"
     CDS             3744..4268
                     /locus_tag="PLT35_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG28090.1"
                     /translation="MTILETDRLAIRRLTLDDATFVLRLLNEPSFIQHIGDRNVRNLA
                     DAKRYILAGPIASYQRHGYGGFLVEMKQTHLPIGLCGLVRRDALDDVELRCAFVPESW
                     SKGYAFESASAMLGYAHDRLGLRRVVAVVSPENSATIGLLVKLGFYFDRMARLPGEKG
                     ESRIFALNFPEALA"
     gene            4347..4432
                     /locus_tag="PLT35_02320"
     tRNA            4347..4432
                     /locus_tag="PLT35_02320"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4381..4383,aa:Leu,seq:taa)
     gene            4991..5464
                     /locus_tag="PLT35_02325"
     CDS             4991..5464
                     /locus_tag="PLT35_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28091.1"
                     /translation="MKPTTLVIAAGVVVAGLALGAPVHAQSARITVGAVFSTGPQYWP
                     QGPYPGRPGPGAYGAYRGFGAREFAQNRGFADGYEQGFDAAADRNRFDPWRERWYRGG
                     TRGYDRSLRMTRDEYRSFYRRGFLQGYEAGYRDGQRGWRGGYDRRWLDDWRRGWR"
     gene            6161..6457
                     /locus_tag="PLT35_02330"
     CDS             6161..6457
                     /locus_tag="PLT35_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28092.1"
                     /translation="MAIPPTEEWRGNALDVARPSVVKSKARCVWSLWNSGTVAHAELH
                     EHPLGHQLCVYLDGSLLFTSVHATREAAEREAMGLKQKAMAEAWTDPWSPRTAD"
     gene            6598..7191
                     /locus_tag="PLT35_02335"
     CDS             6598..7191
                     /locus_tag="PLT35_02335"
                     /inference="COORDINATES: protein motif:HMM:NF040142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28093.1"
                     /translation="MKRGTPKGPQPLKRNWLPLAAVAAGYAVAFVLIRRFTPVSLDSP
                     WFVVAAMACCLGLLFAAQPAVMIRMPRRLRTLRAWEREGGFYRSIGVPAFGRLLRRTP
                     LRLFNTDVYLGNGLSRAANVAAELEAAEASHLIAAALLLPYMMKIALEGRWAALAWVS
                     LAQLLVNVYPVLHLRLTRRRFERVAVRLSASGTGRRD"
     gene            complement(7263..9065)
                     /locus_tag="PLT35_02340"
     CDS             complement(7263..9065)
                     /locus_tag="PLT35_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28094.1"
                     /translation="MRNLKPVTVTLALLAAAPASAQYRTLDDHFAPPRFTSRADWEAR
                     AAYLREHVLASAGLLPMPPKTPLNPVVFDETTHDGYSVSKVYFESLPGFYVTGNLYKP
                     AGEGPFPAVLSPHGHWAYGRLENSATGSLPGRGINLARLGMVVFMPDMVGYNDSRQLP
                     HTFGGKRESLWGLSLAGLQLWNNIRGLDFLQSLPYVRKDALGATGASGGGSQTFLLAA
                     VDERVAVSVPVNMISLHMQGGCLCENMPGLRLDTTNVEIAAAIAPRPLLMVSATGDWT
                     RDTLEKEYPAVRAIYALLGAEDRVSAVRFTAEHNYNKDSREAMYAWIARWLQGAPAQP
                     RLEERAFSVDRLQDLLVFHGRALPEGALTPDAITAGWIGAARQQLTATPLSVRASALR
                     HALAFAAERAVTPGGAGAAAPGAAAPGAAARAPARRRPAVLIAGDVGGAGDALRAAGF
                     TVRPIAFAAFDAEAAARIKHFDTYNRTPASQRVADIVSALRAEPNAVLVAAGDEALAG
                     ALASAIAPPRLAVLDVKGFDAGRDEEFVARVYIPGLRRAGDFWTAADLAPGRLVIHGA
                     GEGFDGTGARIHRSALSAADIVALVREEAKRPLR"
     gene            complement(9078..10067)
                     /locus_tag="PLT35_02345"
     CDS             complement(9078..10067)
                     /locus_tag="PLT35_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28095.1"
                     /translation="MQDPAGPAQTAGAFVALRPRRPRVALPPGCLTLLGGPLMACGLW
                     QIGLVAFARPDALAARGLLGFGLAFAAVSAAIFFVRMRRTLSALTPVPDVSLEGGHRL
                     TPGAIVPMRIRLRAAARLARLKITLVCERRYVDQETTPGTMSVRPVERIETLTTQDLF
                     EETGLSLNRRILWEKTASVVVPYLAKPSGPVLPSGTAEWYIDVLTEPSIGKAVHDRYD
                     LVVVLSDAPDTPLQPETPGARPRARAAPATLARIGPAVGCAVLALAFLFAGPILPWFF
                     FSGIPTRRGSLLMALAVGILFAGLVLAGVSLLVHRAVRPDNWKGRTHDPRRLP"
     gene            complement(10156..11421)
                     /locus_tag="PLT35_02350"
     CDS             complement(10156..11421)
                     /locus_tag="PLT35_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG28096.1"
                     /translation="MNRKEFLKRLAAAVGGFAVPSLASAASFERVLQDASAGSGEALW
                     KVVRDQFVLDPDWTYLNFGGLGSCPLPVLTSFWEWTRSEERAPNAGHDAALWWSVKER
                     LAKLLGPACRKEDLALISCATEGCNIIASGLPLRRGDEVITSRHEHVALNTALLHRAQ
                     QDGIVIRVFEPDLVRAQGNVERVAALVNGRTRLIFMSHVTCTTGQLYPVKEIAALAHE
                     RGIWLALDGAQAPVCVPFDITECGADFYTCSTHKWIMGPKRTGFLWVRPGLLDTLRPL
                     VPGSAKFDVDKGEMAFQADAQRYEFGSQNDALFFAMGAAVDFVQAIGPGRIWEHNHRL
                     AERFYRGLQEIPAAEIASPQEEAFRTAMISFRMKTRTVQQIMEHTAKAKIRVRPVSEG
                     GANCVRVSFHVCNQEAEADRLLDVLRQFA"
BASE COUNT         1726 a         4170 c         4222 g         1600 t
ORIGIN      
        1 cgcttcatct gcgacttgaa gctgccgccg cccgcgttca ccacgacggt gtggcccgcg
       61 tcgcgcagcg tctcgccgac gcgacgcgcg acgggcgccg cggctgcgcc gtcgtgcacg
      121 acgtacgccg ccggcgcgtg cccggccgcg gcgcgaggtt cagtcgccgg ctggcgcgct
      181 gccggggggc tcctcccaga agtcgcgcgg ccccgcgtcg atgccggcga gggcctcgcc
      241 gcgcaggatg cggtccgcca tggtcaggcc ctggccgatt gagtggtcca tgttgttgta
      301 ccagaaccgc ccgcaccggc cggcgagcag gagattgcgg acgcccccga gcgcccgcag
      361 gcaggatgcg agttcggccg ggtagtccag cccgtagatg ggataggtgt tgcgcacggg
      421 ctcgatgtgc agcccgcgga cgtcgtcggg gctgccaatc atgcccgtcc gcgccaggtc
      481 gcgcacgatc ccgtcgacga gcgcctccgg cccgccccag cgctcgtccc cctcccggca
      541 cgttacctcg acgcacaggc cgctcatccc gtccggcgcc atgccccggt cgaacgctgt
      601 cggcgacgac gcgcgcgaga agatctcgtc gccgccgaag tacgtccact ggtacggcac
      661 cctcggcggg acgccaatct cgaagttgta gaagagcgtc gagaggaact cgagacgcac
      721 cggcccgatg cccgcaagcg ccgcggcctc ggtgatcggg cccgtccaca cgacgtcgtc
      781 gcacgcgatc tcttcgcccc cggccgccac cgccgtgatc cgcccgtctc gcaacgtcag
      841 cgcggtgacc ctgcgaccga ggagcacgcg cccgccgcag tcccggatcg ccgagaccag
      901 ccggtccgag aactgcccga ttccacgcgg gctgtacagg aacgtggtct cgaccggctt
      961 cggcaggagc gtcgtccgga tgagcgacca gatcgtgtcg gccgacgcgc gcttgtcgat
     1021 gacggcccgg ttcacgccgg cccggcccca gtcccggtgc aagtccgccg ggtccgagcc
     1081 caggaacttc cgggtgtagg gacggaagaa gatctcgtac agcgtgcggc cgtacttgtt
     1141 gagcacgtcg gcttcgaacg actcgccggg cacgcggcgc cgcgcgacgg cgtcgatcgc
     1201 gccgcgcagc atgacgctcg gcggcatcga gaggaacacg ctcgggcgca ggggccacgg
     1261 gtggaagcgc ccgaacatgc gcgccccgct cgaccgcgag atctcgatcg cctgggaccc
     1321 gaggacctcg cggacgaact gctccacccg gcggttgtcg gtgtggaagc gatgcgggcc
     1381 cacgtcgaaa tggaagtcgc cgtagtgcca ggtccgggcg aggccgccgg cccaggcgtc
     1441 ccgttcgacg agcgtcacgc gccggccctc gcgggcgagc cggctggcga tggtgaggcc
     1501 ggcgacgccg gccccgacaa cgacggtctg ggtgttcact gtacggagta gatgcggact
     1561 tccgggttct gaaagacctt cgtgaacaga tccaggcggc gatcgaactt cacgccggcc
     1621 tccgggtagt gctcgagttc aacctggcca aggtacaagt acctgatgcc catggagcgc
     1681 gcgatgcgat gcgccagatc cgggtcctcc gtcgcataga gctcgcggac gcgctcgctc
     1741 gcccgatggt aggcgggctc gtcgagcagc gagatcggaa gaccggtcgc catccggcgc
     1801 tcggcgaacg acgggatgag cgtccagctc gcgcggcccc gcgcggtggg ctccacctgg
     1861 acggtctgcc acggcttcgt gtggcgcttg atccagtcga gcgcctcctg ttcgttcctc
     1921 gtcacgacca cggtccacgg gaagccgggg cccatcgtgc gtgtctcgac atcctgcgcg
     1981 ttgatgtagt ccagcaccgt cgtcggcact cccgcgagcg ccacgccggc cgcgagggcg
     2041 attgcggccc agcgccggcg atcccacagg gccgcgaagc ccaacgcgcc gaggcccggc
     2101 gccgcgacga acatcacctg gccggcccgc cacccgatcc agggctcggc cgacgtcagc
     2161 gtgacgaaga agatcagcag gaacgcggcg accacggcga gcagggccgg ccgcgcgctg
     2221 cgtggcaggc gccaggatgc cgcgaggccg gccagcgccg gggcgaggat gggcccggcc
     2281 gccagcggga tggcgacgag cggcgccctc atggccagcc tggacagccc gaaccgaagg
     2341 ttcgacccgg tgccctccag cgtctggttc gccaccatcc acccgacggc gccgatgacg
     2401 ggcaggccgg cgagggcaag cgtcgcgacc ttcgagcaga agcgttcgcg gtcgatgacc
     2461 aggtcggcgg cggccgcgag tccccacgtg aggctgatga ggccgccggg gaacgggctg
     2521 accatcagcg ccaggccgag cgaggcgccg cacgccaggg cgacgccgcc acccatccgc
     2581 gctccggccc tggcgacgat gaccagcgcc accaggccga cggcgcacgc catcgagtgg
     2641 tgggtgttgt agtagagcga ccgctgcagc ccgtcgatcg acaccgcctg gaagaaccag
     2701 taggtggtgg cgtcgacgtt gatctcgcga atcgcggcaa gcggcgcgcc gccgcgcagg
     2761 cagcgctgga tcaagtacag cccctccgcg ctcgctgcca cgaaggcgag cgcgacggcg
     2821 acggccgacg ggccggcgcg gcctccgcag gccatccacg cgaacaggaa gaccatggcg
     2881 gcgaagatca tgccggtgcc gagcgcgttg accgtgagcc ggccgacccg gtcgggcagg
     2941 atatggggca tgacgagtgc tgccgacgat ggcggcacga agtgaagcca gtagtagtgg
     3001 agcgtgtcct tcgcccggta cggattgcgc ggcgggttgt ctgctttctc gagctccgcc
     3061 gtcagggcca tgtgccacac gaagtcggcg gtgaaatagg cgcggaacag ccgctctccg
     3121 gccgcgtcgc gctcgccgat gcgggagaac ggccgcccga caagcagcgg cacgagcgcg
     3181 acgaccatcg ccagcgcggc cgtatcacgg cgggtccatc gaggcagatc gatgcgccgg
     3241 gcccgtcgcg ggaagagcac cgcccagatg accgccgtgc cggctgccca cgtgctgact
     3301 atcgtctccg ggcggggaac gccgagcgcg atgggaatcc agagcaggaa tgccgtcagg
     3361 ggatacccca gggcgaaccc ggcgatccac gtggcgccat gcctggcgcc gaacacgtag
     3421 gcggccaccg gccatcccgg ggcgattgag gcgacgacga gagcgagaca gacgatcccg
     3481 ccccagcccg atcccaggcg ccaggccagg gccagcaccc acacgccgac ggcggcgatg
     3541 agaaccgggg cagcacggcc ggcagtcagg aaatccggaa ggaagaggcg accccgcctg
     3601 tctgctgtcg aggtccggtc cggcatcacc tcagaagctt agcagaaact tccctctctt
     3661 tccctctttg agccactcgc tggcacgaag cgggccgaca gggccggcct cgccgtataa
     3721 ttggaggcga ggagggcgct cccgtgacca tcctcgagac cgaccgtctg gcgatccggc
     3781 ggctgacgct ggacgatgcg acgttcgtgc tccggctgct gaacgagccg tccttcatcc
     3841 agcacatcgg cgaccgcaac gtccgcaacc tggccgacgc gaagcggtac atcctggctg
     3901 gccccatcgc cagctaccag cgccacggct acggcgggtt cctggtcgag atgaagcaga
     3961 cgcacctgcc catcggcctc tgcggcctgg tcaggcgcga cgcgctcgac gatgtggaac
     4021 tgcggtgcgc gttcgtgccg gagtcgtggt cgaagggcta cgccttcgag tccgcgtccg
     4081 cgatgctcgg ctacgcgcac gatcgcctgg gattgaggcg cgtcgtcgcc gtggtgtcgc
     4141 ccgagaacag cgcgacgatc ggcctgctcg tgaagctggg cttctacttc gatcgcatgg
     4201 cacgtttgcc cggcgaaaag ggcgagtcga ggatctttgc gctgaacttt cccgaggcgc
     4261 tggcgtagcc tggaagagag cgggagccgt ggcggccgcc tgccgcggcc actgcgcctt
     4321 tcagctatga tcgggccgcg tcctgggccg gggtggcgga actggtagac gcacgggact
     4381 taaaatctcg tgagggtgat acctcgtgtg ggttcaaacc ccacccccgg cacttccaac
     4441 cgcgttcctg cccgccattc aacggctccg ggatctgacc cggctcctct gacacggcgt
     4501 gcggctcgga cgcgccggtg cccgtgccgg cggcggccgg caatccgtga cagcggcggg
     4561 cttcgtcagg gataccggcg ggagggccct gctcatcgag gccggggcgg acgcgccgcc
     4621 gagcctgacc ggccgtgcct ctcgctgcgc gttcaggggc gagggcggct gggaggcgcg
     4681 cgccgtgccg ctccccccac gctgcggcag ccggcccgaa cgtccggcgc cagacgcgcc
     4741 ggccgtgccc cggcggatgc tccttcgaga gggagaagcg acgcgccgcc gtgtcctcga
     4801 ccgggcgcac gcgtccacgc cagttgactc ccgcatcctc cagcgacggc gtcgatcgct
     4861 cgatttccgg cctgatcccg cgggaattgc gatggcccgg gccttgcacg atacgttggc
     4921 agaaggcagg gccggagcgg cggcacggac caacgtccga accaccgcgc tcacggagat
     4981 ggaggacgcc atgaaaccga cgacgctcgt catcgcagcc ggggtggtgg tggctggcct
     5041 ggcgctcggc gcgccggtgc atgcgcagtc ggcgcggatc accgtgggcg ctgtattcag
     5101 caccggcccg cagtactggc cgcagggccc gtacccgggg cggcccggcc ccggcgccta
     5161 cggagcctat cgcggattcg gcgcgcgcga gttcgcgcag aaccgcggct tcgccgacgg
     5221 ctacgagcag ggattcgacg ccgccgccga tcgcaaccgc ttcgatccgt ggcgcgagcg
     5281 ctggtaccgg ggcggcacgc gcggatacga ccgcagcctg cgcatgacac gcgacgagta
     5341 ccggagcttt taccggcgcg gcttcctgca gggctacgag gcggggtacc gcgacgggca
     5401 gcgcggctgg cgcggcggct acgaccgccg gtggctcgac gactggcgga ggggctggcg
     5461 gtagtccgcc ggcaaacggg ggcggctgca tgggccgccc cagcgccttc gcgccggcgc
     5521 gttcatcacg cgcggcctgc gactcccgaa tccaccgaat ccacacggcc cacctcggca
     5581 gcggcccggc tcgtccattc cccagcccgc gcggccgagg ccgccgctcc ggcttcgcag
     5641 ccggcatcga ggccggcgtg gccgcgccgt cgtcggcctc cgcctgcagc acgaactcgc
     5701 caggcgccgt caccggcacg ggcgtgacag gggcggatga atcggccgaa caaacaggcc
     5761 aggatccgcc aacccgaccg gagcgtggca cgcacaggca gccaggctgg atttgtcaga
     5821 atacctgaca gatcctgagc gagccgaaaa gcggctcgat ctctctctac tggcgtatct
     5881 gtttcgatac aaaagccttg tgcgcaacgg gccgatgagg cgccgacttc cgcctggctg
     5941 gcgtgatgtt tgctggggcg gcagtcgggt cggctggctc cttcggcgag cgcgtcggtt
     6001 ggtggagaga aagcccgggc tgcggtctgg tgaacgcttc tcggttcgcg gacggcgcgg
     6061 aagccgtctc ccggtcggct tcagccggcc ggcgcgagcg tcacgaggct ccagctgctg
     6121 gcaggcgggc gcagcggcgc accgccggga gtctgtggac atggccattc ccccgactga
     6181 ggagtggcga ggcaatgccc tcgacgtcgc caggccgtct gttgtgaagt ccaaggcccg
     6241 ctgcgtgtgg tcgctctgga actcgggcac ggtggcccac gccgagttgc acgagcatcc
     6301 gctcgggcac cagttgtgcg tctacctgga cggcagtctc ctcttcacgt cggtccacgc
     6361 gacgcgcgag gctgccgagc gggaggcgat gggcttgaag cagaaggcga tggccgaggc
     6421 ctggaccgac ccctggtcgc cccgcaccgc ggactgactc ccccgctcga accggctcgg
     6481 cgtgcgcccg gacagccctg ccggccttga tccggctcat ccggcccacg ccagggtcgg
     6541 actgctcatc ggccgcgttc acgcgccgcg ccggcccgat cgcgctatgc tgctcccgtg
     6601 aagcgcggaa cgccgaaagg gccgcagccg ctgaaacgga actggctgcc gcttgcggcc
     6661 gtcgcggcgg gctacgccgt ggccttcgtc ctcatccggc gcttcacgcc ggtcagcctg
     6721 gattcgccgt ggttcgtcgt ggccgcgatg gcgtgctgcc tcgggctgct gttcgcggcg
     6781 cagccggccg tgatgatccg gatgccgcgc cggctccgga cgcttcgggc ctgggagcgc
     6841 gagggcggct tctaccgctc gattggcgtg ccggccttcg ggcgtctgct gcggcggacg
     6901 ccgctgcggc tgttcaacac ggacgtgtat ctgggcaacg ggctgtcccg ggcggcgaac
     6961 gtggccgccg agctcgaagc ggcggaggcc tcgcacctga tcgcggccgc gctgcttctg
     7021 ccgtacatga tgaagatcgc gctggagggg cgctgggcgg ccctggcctg ggtgtcgctc
     7081 gcccagctgc tcgtcaacgt ctaccccgtc ctgcatctgc gcctgacgcg gcgccgcttc
     7141 gagcgcgtcg cggtccggct gagcgcgagc ggcacaggcc gccgcgactg acgctccccc
     7201 gaagcgcgcc ccggcgcgtc ctgctccgac cggctatcgc ggccgagcgc gctcggacgc
     7261 cgtcagcgaa gcgggcgctt cgcctcctcc cggacgaggg ccacgatgtc cgccgcgctc
     7321 agcgcggacc gatggatgcg cgcgcccgtc ccatcgaagc cctcgccggc gccgtggatc
     7381 accaggcggc ccggcgccag gtcggcggct gtccagaagt cgccggcgcg gcgcaggccg
     7441 gggatgtaga cgcgcgccac gaactcctcg tcgcggcccg catcgaagcc cttcacgtcg
     7501 agcacggcca ggcgcggggg cgcgatagcc gacgcgagcg cgccggccag ggcctcgtcg
     7561 ccggcggcca ccaggacggc gttcggctcg gcgcgaagcg ccgagacgat gtccgcgacg
     7621 cgctggctgg caggcgttcg attgtacgtg tcgaaatgct tgatcctggc ggccgcctcc
     7681 gcgtcgaacg ccgcgaacgc gatcgggcgg accgtgaagc ccgccgcgcg cagcgcgtcg
     7741 ccagccccgc ccacgtctcc ggcaatcaga accgcgggcc ggcggcgggc cggcgcccgt
     7801 gccgcagccc cgggggccgc ggcgccgggg gccgcggcgc cggccccgcc cggcgtgacg
     7861 gcgcgctcgg ccgcgaaggc cagcgcgtgc cggagcgccg aggcgcgaac cgacagcggc
     7921 gtcgcggtca gctgctgcct ggccgcgccg atccagcccg ccgtgatggc gtccggggtg
     7981 agcgccccct ccggaagcgc ccggccgtgg aacacgagca ggtcctgcag gcgatccacg
     8041 gaaaaggccc gctcttccag ccgcggctgg gccggcgcgc cctgcagcca gcgcgctatc
     8101 caggcgtaca tcgcctcgcg gctgtccttg ttgtagttgt gctcggccgt gaaccgcacg
     8161 gcgctcacgc gatcctccgc tccgaggagc gcgtagatcg cgcgcaccgc cggatactcc
     8221 ttctcgaggg tgtccctggt ccagtcgccg gtggccgaca ccatcagcag gggccgcggc
     8281 gcgatcgccg cggcaatctc gacgttggtc gtgtcgaggc ggaggcccgg catgttctcg
     8341 cacaggcagc cgccctgcat gtgcagcgag atcatgttca ccggcacgga cactgcgacg
     8401 cgctcgtcca cggccgccag caggaacgtc tggctgccgc cgcccgacgc gccggtcgcg
     8461 ccgagggcgt ccttgcggac gtagggcagc gactggagga agtccaggcc gcggatgttg
     8521 ttccagagct gcagcccggc caggctcagg ccccagaggc tttcgcgctt cccgccgaag
     8581 gtgtgcggca gctggcggct gtcgttgtag cccaccatgt cgggcatgaa cacgaccatg
     8641 ccgaggcgcg cgaggttgat gccgcgtccc gggagcgatc cggtcgccga gttctcgagc
     8701 cggccgtacg cccagtgccc gtgcggcgac agcaccgccg ggaacgggcc ctcgccggcg
     8761 ggcttgtaga ggttgcccgt cacgtagaag ccgggcaggc tctcgaagta gaccttcgac
     8821 acgctgtagc cgtcgtgcgt cgtctcgtcg aagacgaccg ggttcagggg cgtcttcggc
     8881 ggcatgggca gcaggcccgc tgacgccagc acgtgttcgc gcaagtacgc cgcgcgcgcc
     8941 tcccagtcgg cccgcgacgt gaagcggggc ggcgcgaagt ggtcgtcgag ggtgcggtac
     9001 tgcgccgagg ccggggccgc ggcgaggagc gcgagcgtga cggtgacggg tttcaggttc
     9061 cgcatgcgcg gagggtatca cgggaggcgc ctgggatcgt gcgtgcgccc tttccagttg
     9121 tcaggcctga ccgcgcggtg aacgagcaac gagacgccgg ccaggaccag gccagcaaac
     9181 aagatcccga cggcgagcgc catcaggagg ctgccgcgcc tggtcgggat gccgctgaag
     9241 aagaaccacg gcaggatcgg cccggcgaac aggaacgcga gagcgagaac ggcgcagccg
     9301 acggccggcc cgatccgagc gagagtcgcc ggtgcggcac gggccctggg tctcgcgccc
     9361 ggcgtttccg gctgcagcgg cgtgtcggga gcgtcgctca ggacgacgac caggtcgtag
     9421 cggtcgtgga cggctttgcc gatggacggc tcggtgagga cgtcgatgta ccactcagcc
     9481 gtcccggacg gaagcaccgg gccgctcggc ttcgccaggt acggcaccac gaccgaagcc
     9541 gtcttctccc acaagatcct gcggttgagc gagaggcccg tctcctcgaa caggtcctgc
     9601 gtcgtgagcg tctcgatgcg ctcgaccggc ctgaccgaca tcgtgcccgg tgtcgtttcc
     9661 tggtccacgt accggcgctc gcataccagg gtgatcttca gccgcgccag ccgcgccgcg
     9721 gcgcgcaggc ggatcctcat cggcacgatc gcgccgggcg tcagccggtg tccgccctcg
     9781 aggctgacgt ccggcacggg cgtgagcgcg ctgagcgtcc gccgcatccg gacgaagaag
     9841 atggccgcgc tgacggccgc gaaggcgagg ccgaaaccga gcagcccgcg ggcggcgagc
     9901 gcgtccggcc gggcgaacgc caccagcccg atctgccaca gcccgcaggc catcagcggc
     9961 ccgcccagca gggtcaggca ccccggcggc agcgccacgc gcgggcgccg cggtctgagc
    10021 gccacgaacg cgcccgccgt ctgcgccggt ccggcaggat cctgcatgcg tgtctccgca
    10081 ggggccgcag ccccaccgcg gtttcggcca accacccagg ggccgggccc ctcggggaga
    10141 ggccgggccc ttccgctacg cgaactggcg gagcacgtcg aggaggcggt cggcttcagc
    10201 ttcctggttg cagacgtgga acgacacgcg gacgcagttg gcgcccccct cggacaccgg
    10261 gcgcacccgg atcttcgcct tcgcggtgtg ctccatgatc tgctgcacgg tgcgcgtctt
    10321 catccggaag ctgatcatcg ccgtccggaa cgcctcctcc tgcggcgagg cgatctcggc
    10381 ggccgggatc tcctgcaggc cgcggtagaa ccgctcggcc agccggtggt tgtgctccca
    10441 gatgcggccc gggccgatgg cctgcacgaa gtcgacggcc gcgcccatgg cgaagaagag
    10501 cgcgtcgttc tgcgacccga actcgtagcg ctgcgcgtcg gcctggaacg ccatctcgcc
    10561 cttgtcgaca tcgaacttcg ccgagcccgg caccagcggc cgcagcgtgt cgagcaggcc
    10621 cgggcgcacc cacaggaacc cggtgcgctt cggccccatg atccacttgt gcgtgctgca
    10681 ggtgtagaag tcggccccgc actcggtgat gtcgaagggc acgcagacgg gggcctgcgc
    10741 gccgtcgagc gcgagccaga tgcccctctc gtgcgcgagc gcggcgatct ccttcaccgg
    10801 gtagagctgg cccgtcgtgc acgtgacgtg gctcatgaag atgagcctcg tccggccgtt
    10861 gaccagggcg gcgacgcgct cgacgttccc ctgcgcccgg accaggtccg gctcgaacac
    10921 cctgatgacg atgccgtcct gctgggcgcg gtgcaggagc gccgtgttga gggcgacgtg
    10981 ctcgtgccgg gacgtgatga cctcgtcgcc gcgcctcagg ggcagccccg aggcgatgat
    11041 gttgcagccc tcggtggcgc agctgatcag ggcgagatcc tccttgcggc acgccggccc
    11101 gagcagcttc gccagccgct ctttgacgct ccaccagagc gcggcgtcgt ggcccgcgtt
    11161 cggcgcccgc tcctcgctcc tggtccattc ccagaagctc gtcagcacgg ggagcgggca
    11221 cgacccgagc ccgccgaagt tgaggtaggt ccagtcggga tccagcacga actggtcgcg
    11281 gacgaccttc cagagcgcct cgccgcttcc ggccgaagcg tcctgcagca cgcgctcgaa
    11341 ggacgcggcg ctcgcgaggg agggaacggc gaagccgccg acggcggccg ccaggcgttt
    11401 gaggaactct ttgcggttca taccgccgga tcctacagca agacgccgat ggcgcatcgt
    11461 cggggcaggg ctggcccggg acgcgggcct ccgcgcggaa cggcccccac aagcaaagag
    11521 gccggggcag aagccccggc ctctttgtcg aacgacgatg ggcgcgcgcg gcggaacggc
    11581 cgcggcgcgc gccgttcagc gtgctaccac gccacgcgga agccgagctg gacgcggcgc
    11641 ggtgtgaaga ccgcggtgta gtccacgctg cccgggttgt agagcatgaa gttcggcgac
    11701 gtgatcgacg tgttcggc
//
LOCUS       DAOSIF010000035        11374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5653_length_11374_cov_3.757752, whole genome shotgun sequence.
ACCESSION   DAOSIF010000035 DAOSIF010000000
VERSION     DAOSIF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_11374_cov_3.757752"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLT35_02355"
     CDS             <1..678
                     /locus_tag="PLT35_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG28097.1"
                     /translation="TLISAFVDLINRTRSDYIVTIETEIKFVHENRRAMVSQREARSD
                     PDAMLHGARAALRENPDVLVIDDLKTPDLVAVALDAAESGHLVIGAVTAHTTSAAIGR
                     LIETAAYERQTQVALSLSEALRGAVAQVLLRKAGGGRVAARELLFNMPSVGALIAEGK
                     IGQLPLAIDSGRKYGMVPMNDVLLAFVQSGAVDPREAYRKAYDRAAFLDLMKRDGIDT
                     SFVERLA"
     gene            complement(709..1143)
                     /locus_tag="PLT35_02360"
     CDS             complement(709..1143)
                     /locus_tag="PLT35_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28098.1"
                     /translation="MSLELAQIRRRVQTRLADVKRAAAARRDRADAAGRAYGTFLSAV
                     AIPTFSAVAQSLSAEGYPFTVTTPGRAVRMTSDRSRLTYVEIGLDTSGPEPQIIAEVG
                     WERGSRVFTDERPVAEGTPVDAITDEHVLARLLELLPELVER"
     gene            complement(1225..2337)
                     /locus_tag="PLT35_02365"
     CDS             complement(1225..2337)
                     /locus_tag="PLT35_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28099.1"
                     /translation="MVYLDLLLVGFGRVGRRFTELLEEQSVRLRGEYDLSWRVVGIAT
                     RRHGTAYDARGLDLVKARRLAGAGASLASLTGAESGRTIAAEASGLALIRHATAESAA
                     RRMPQLVVVETTTLDVERGQPATDHVMAALRGGAHVVTANKGPVAFAYREIASAAGVA
                     RRRFLFEGTVMDGTPLFSLVRETLPGVRVLGFRGILNATTNYVLTAMEEGRSFERALS
                     DMQEAGIAEADASLDIDGWDAAAKTAAIANVLMRGSVTPRGIERTGIRDISSKRVLDA
                     VSRGRRVRLVASAEWVDDRLAGRVAPVELDEADPLARLAGLQNEVVLKTDLLGPIGIQ
                     QFDGGLAQTAYALLSDLVTIAKGLSRGETSAAPPLR"
     gene            complement(2355..3494)
                     /locus_tag="PLT35_02370"
     CDS             complement(2355..3494)
                     /locus_tag="PLT35_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG28100.1"
                     /translation="MRLPLFAMERMQSTWENLVDYNLSESGVRPMRVEELLDSDADRA
                     AFFAQELAYNQSNGTIALRERIAAMYPGATIEHVEVTNGGSEANFVSMWHLVRPGDEV
                     VSMVPNYGQTLGLAEGFGGALKPWPLRLSEDGRRWLADLDGLRGLVTPKTRLIVLCNP
                     NNPTGARVPAGDLDEICRIAGRVGAWVLSDEIYRGAELDGAETPSVWGRYERVVVTSG
                     LSKAYGLPGLRVGWIVSAPEVVASTWSRHDYTTIAPGTLSDRLARLALEPPTRAKILA
                     RTRGILHANLPVIAGWLDARKDRFSYVPPEAGAIVYTRYRGGMNSTGLVERLRVEKSV
                     LIVPGDHFGMDGHLRIGFGGETRGLRDGLDRLADLLQSLPLAGGA"
     gene            complement(3554..4339)
                     /gene="moeB"
                     /locus_tag="PLT35_02375"
     CDS             complement(3554..4339)
                     /gene="moeB"
                     /locus_tag="PLT35_02375"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HOG28101.1"
                     /translation="MHPGSGFSGEELERYARQLVLPGVGVEGQQRLKAARVLCVGAGG
                     LGSPASLYLAAAGVGTIGLVDSDRVSLSNLQRQVLYGTPEIGAPKTAPARVRLEELNP
                     HVSVEPHQVALAAGNAVDLLGRYDIVLDCSDNFPTRYLINDTCVLLDKPDVFGAVFMF
                     EGQMSVFGRRGGPCYRCVFPEPPPPEIEPSCADSGILGALPGVIGAIQAAEAIKLITG
                     VGEPLVGRMIVCDALAMTFRELGLPRNPRCAACGERPAGCALP"
     gene            4485..4952
                     /locus_tag="PLT35_02380"
     CDS             4485..4952
                     /locus_tag="PLT35_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG28102.1"
                     /translation="MLRLSKKADYALIAMAHLAQQPGSASAREIAETYDIPMELMAKV
                     LQRLVRRGLLTSQQGARGGYRLSQAAESISLADVIQAIDGPVSVTACSSDADCCGQYE
                     KCNVRDPLWRISSIILSALAGTSVSELAGDLADSAPGADPREPARLAQAAIRA"
     gene            4964..6181
                     /locus_tag="PLT35_02385"
     CDS             4964..6181
                     /locus_tag="PLT35_02385"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOG28103.1"
                     /translation="MLKFPIYFDNQATTPVDPRVLEAMLPYFTERFGNAASRNHRYGW
                     EAEEAVEAARKQVADLIGANPKEIVFTSGATESDNLAIRGAVYMYREKGDHIITALTE
                     HKAVIDTCKRLEKEGFRVTYLPVKPDGLVDLDALRDAITAKTVLITIMAANNEIGVLQ
                     PVAEIGAMAKERGVLFHTDAVQAAGKVPFDVNACNAHLASLSAHKMYGPKGVGALYVR
                     RRQPRVLLSPIIDGGGHERGMRSGTLNVPGIVGFGKAAELCRLEMAQESARIRALRDR
                     LNAKLHAGLDELHVNGSMEHRLPGNLNISFAYVEGESLLMGINDVAVSSGSACTSASL
                     EPSYVLKALGTGDDLAHSSIRFGLGRFNTEEEVDYVAGKVVAVVRKLREMSPLYEMAK
                     DGVDLAKMEWREH"
     gene            6274..6672
                     /gene="iscU"
                     /locus_tag="PLT35_02390"
     CDS             6274..6672
                     /gene="iscU"
                     /locus_tag="PLT35_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOG28104.1"
                     /translation="MAYSDKVVDHFNNPRNVGSLPKQDPTVGTGLVGAPECGDVMKLQ
                     VRVNPETGVIDEAKFKTFGCGSAIASSSLATEWLKGKTVDEALTIKNTDIVAELSLPP
                     VKVHCSVLAEDAIKAAIADYQQKQQGGPSS"
     gene            6669..6908
                     /locus_tag="PLT35_02395"
     CDS             6669..6908
                     /locus_tag="PLT35_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOG28105.1"
                     /translation="MIAVTEKAASHIRKLMAKQGITDGGLRVGIKGGGCSGLSYTFSW
                     EKEARLGDEVIEGAGGTRLFVDKKSLIYLTGTTLE"
     gene            7125..7835
                     /gene="hscB"
                     /locus_tag="PLT35_02400"
     CDS             7125..7835
                     /gene="hscB"
                     /locus_tag="PLT35_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOG28106.1"
                     /translation="MQPDTKAVPIDKCRHCGSEAPVDVHVCPRCDRLMTLGRLGDYFT
                     FFGLRRRLVLDVQELDQRYRALSRLFHPDYYHNAPPAERLASLERASYLNDAHRTLRD
                     PAARLEYLLKLEGFAPKQSREAASAVPQGLLEEVFALNEELEAVEELRESGAPAEAVA
                     ARLDEARRPVEATLEAHERRLLELAQAHDASLESGDAAGRRETLGLLETLLLERTYLT
                     NLLAAVRRHEAGAGSART"
     gene            complement(7795..8181)
                     /locus_tag="PLT35_02405"
     CDS             complement(7795..8181)
                     /locus_tag="PLT35_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28107.1"
                     /translation="MKLEISGGVNVRSPRRTRMTLPGSPPSLKGSHCSSPLASSMPRP
                     MNRLTEYTERAASESSRRCASRPTWIAPDSPTDTTDGSSRSPDSSAMTVVAPSRRTAT
                     RLFVVPRSMPTTLLMFARSRRRPRGA"
     gene            8086..9699
                     /gene="hscA"
                     /locus_tag="PLT35_02410"
     CDS             8086..9699
                     /gene="hscA"
                     /locus_tag="PLT35_02410"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOG28108.1"
                     /translation="MAALQAGRRSRERHPRPPGRADVHAARDLELHPPRAEAPRRGLL
                     RQGGDIDPEVSAAVITVPAYFNDAQRTATRDAGRLAGLEVLRIVNEPTAACLAYGLDR
                     RRQGVVAVYDLGGGTFDISILRIEDGVFQVLATNGNTHLGGDDFDRLLVEMVARDTGI
                     DPAAAPEAVQAIRKAVIQAKLDLSEFEETRIEIDSLPGRAGPYSRRLTRDEFEAIIGP
                     VVARTLEPCRKALADAGLDPGQVDEAVLVGGSTRVPLVRREVERLFGRAPHTDLNPDE
                     VVAIGAAVQADILVSGRREMLLLDVTPLSLGIETMGGVVSKIILRNSTIPASGGEMFT
                     TFVDNQTAVDIHILQGEREMASDNRSLGRFKLRGIPPMPAGLPRIQVRFQIDANGILS
                     VSARELRTGIAQTIEVKPSYGLTDEEVERMLIESFEHAQEDVEARMLIDARNEAETII
                     AATEKALRRPEFGAIAAADLDPKELVLIHAALADLKQSMGGGDRYAIQVKAAALNQVT
                     RHLAEVMMNRTVQAALAGRAAADVAAEGR"
     gene            9700..10143
                     /locus_tag="PLT35_02415"
     CDS             9700..10143
                     /locus_tag="PLT35_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG28109.1"
                     /translation="MPTVTFIVEGQERTFDVDLPSLPYSHHGLQGSLLDIAKNLDVPL
                     EHACGGSCACTTCHVIVREGAGNLSDMQDDEADRLDTAWDLTPQSRLGCQAVVHGDVV
                     CEIPMYTRNYVQEGGGIRLGRRAGTSEAAPAAEPDSAGAKREKLP"
     gene            10140..10349
                     /gene="iscX"
                     /locus_tag="PLT35_02420"
     CDS             10140..10349
                     /gene="iscX"
                     /locus_tag="PLT35_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HOG28110.1"
                     /translation="MKWTDAEEIGIALDEQYPDDDPLAVRFADLRGRVLGLEGFDDDP
                     QASNEPRLEAILLAWLEERKSRLGE"
     gene            10421..11014
                     /locus_tag="PLT35_02425"
     CDS             10421..11014
                     /locus_tag="PLT35_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28111.1"
                     /translation="MRAVAICALALACGLSPVASDAQPEPAAAPTEKPRPTPASPAQA
                     PEQSQPGTSTQAPADAPRSPAEAAVSAVSLERIKRGLSQAPPPPSSRAPLKLEYYVEV
                     LGLAPPFSLFEMYDMDTGRIPYSAPSYSDMRNVLTPAEFRAPVVWLNRATRSGTRMIL
                     DRSAQRSRARRAEEDRRKALQAERDRQAQLKNSIVVK"
     gene            complement(11007..>11374)
                     /locus_tag="PLT35_02430"
     CDS             complement(11007..>11374)
                     /locus_tag="PLT35_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28112.1"
                     /translation="LDLAGADNAFGDVPRESTMAATEAILARRPDVIIELHYDERATE
                     RDADLERLAWKALPGVPAVKNGRVHVLYGGAFVVPGPRVVLTARAFARLLHGVRSGIP
                     SSGTASAPDAGIPDLTPVT"
BASE COUNT         1780 a         3962 c         4105 g         1527 t
ORIGIN      
        1 acgctcattt cggctttcgt cgatctcatc aaccgcacgc gctccgatta catcgtcacc
       61 atcgagaccg agatcaagtt cgtgcacgag aaccggcgcg cgatggtcag ccagcgggag
      121 gcgcggagcg atccggatgc gatgctgcac ggcgcccggg cggcgctgcg cgagaacccg
      181 gacgtcctgg tgatcgacga cctgaagacg ccggacctcg tagccgtcgc cctcgacgcg
      241 gccgagtcgg gccacctcgt catcggcgcc gtcactgccc acacgaccag cgcggcgatc
      301 gggcgcctca tcgagacggc ggcctacgag cggcagacgc aggtcgcgct gtcgctgtcg
      361 gaggcccttc gcggggcggt ggcgcaggtc ctgctgcgca aggcgggcgg cggccgcgtg
      421 gccgcgcgcg agctgctctt caacatgccg tcggtcgggg cgctcatcgc ggagggcaag
      481 atcggccagc tgccgctcgc catcgactcg gggcgcaagt acgggatggt cccgatgaac
      541 gacgtcctgc tcgccttcgt ccagagcggc gccgtcgatc cgcgcgaggc ctaccgcaag
      601 gcgtacgacc gcgcggcgtt cctcgatctc atgaagcgcg acggcatcga cacgtcgttc
      661 gtcgagcgcc tcgcctgatc ccccgcgccg ggcggcggcg cgttgccctc agcgttcgac
      721 cagctccggc aagagctcca gcagccgcgc gaggacgtgc tcgtcggtga tcgcatcgac
      781 cggcgtgccc tcggccaccg gccgctcgtc ggtgaacacg cggctgccgc gctcccagcc
      841 gacctccgcg atgatctgcg gctccgggcc ggacgtgtcg aggccgatct cgacgtaggt
      901 caggcgcgag cggtccgacg tcatgcggac cgcgcggccg ggcgtggtca ccgtgaacgg
      961 gtagccctcg gccgagaggc tctgggccac ggcgctgaag gtggggatgg cgacggccga
     1021 caggaacgtg ccgtacgcgc gcccggcggc atcggcccgg tcgcgcctgg cggcggccgc
     1081 ccgcttcacg tcggcgaggc gcgtctggac gcggcggcga atctgcgcga gttccagcga
     1141 catgggcgtg gctccgggcc cccggtcgcc ggcgcccgcg cggggccggc cggttgccgt
     1201 ttggcgacgg cgccggctgc cccgtcagcg gagcggcggc gcggccgagg tctcgccgcg
     1261 ggaaagcccc ttggcgatcg tcacgagatc ggacagcagc gcgtacgccg tctgcgccag
     1321 gccgccgtcg aactgctgga tgccgatcgg gccgagcagg tccgtcttca gcacgacctc
     1381 gttctgcagc ccggcgaggc gcgcgagcgg atccgcctcg tccagctcca cgggcgcgac
     1441 ccggccggcg agccggtcgt ccacccactc cgcgctggcc acgagccgga cccgccggcc
     1501 gcgcgagacg gcgtcgagca cccgtttgct gctgatgtcc cggatgcccg ttcgctcgat
     1561 cccgcgcggc gtcacgctgc cgcgcatgag cacgttggcg atggccgccg tcttcgcggc
     1621 ggcgtcccag ccgtcgatgt cgagcgatgc gtcggcctcg gcgatgcccg cctcctgcat
     1681 gtcggacaag gcccgctcga agctgcggcc ctcttccatc gccgtgagca cgtagttcgt
     1741 cgtggcgttc aggatgccgc ggaagccaag cacccggacg ccgggaagcg tctcgcgcac
     1801 gaggctgaag aggggggtgc cgtccatcac cgtcccctcg aagaggaacc gccggcgcgc
     1861 caccccggcc gccgacgcga tctcgcggta cgcgaaggcc acgggcccct tgttggctgt
     1921 cacgacgtgg gcgcccccgc gcagggcggc catgacgtgg tccgtcgccg gctgcccgcg
     1981 ctcgacgtcg agcgtcgtgg tctcgacgac cacgagctgc ggcatgcgcc gggcggccga
     2041 ttccgccgtc gcgtgccgga tgagcgcgag gcccgaggcc tcggcggcga tcgtgcgccc
     2101 gctctcggca cccgtcaggc ttgccagcga agcgcccgcc ccggcaaggc ggcgcgcctt
     2161 gacaaggtcg agccctcgcg cgtcgtacgc cgtgccgtgg cgcctggtcg cgatgccgac
     2221 aacgcgccag gagaggtcgt actcgcctcg caggcggacc gactgttcct cgaggagctc
     2281 ggtgaagcgg cggcccacgc ggccgaaccc gacgagcagg aggtccaggt acaccatggt
     2341 ccggcctgcc gccttcaggc gcccccggcc agcggcagcg attggaggag atccgccagg
     2401 cggtccaggc cgtcgcgcaa gccgcgcgtc tcgccgccga acccgatccg caggtgcccg
     2461 tccatgccga agtgatcgcc cggcacgatg agcacgctct tctccacgcg cagccgttcc
     2521 acgagtcccg tggagttcat cccgccccga tagcgcgtgt agacgatcgc gccggcctcg
     2581 ggcggaacgt agctgaaacg gtccttgcgg gcgtcgagcc agcccgcgat gacgggcaga
     2641 ttggcgtgaa ggatgccgcg cgtgcgggcg aggatcttcg cgcgcgtcgg gggctcgagc
     2701 gcgaggcgcg ccaggcggtc gctcagcgtg cccggggcga tggtcgtgta gtcgtggcgc
     2761 gaccacgtcg aggccacgac ctcgggcgcg ctcacgatcc agcccacccg caggccgggc
     2821 aggccgtacg ccttcgacag gccgctcgtg acgacgacgc gctcgtaccg cccccagacc
     2881 gacggagtct cggcgccgtc cagctcggcg ccgcggtaga tctcgtccga cagcacccac
     2941 gcgccgacgc gccccgcaat ccggcagatc tcgtcgaggt cgcccgccgg cacgcgggcg
     3001 ccggtcgggt tgttcggatt gcacaggacg atcaaacgcg tcttcggcgt caccaggccc
     3061 cggaggccgt cgagatcggc cagccagcgg cggccgtcct ccgacagccg gagcggccac
     3121 ggcttgagcg cgccgccgaa gccttcggcg aggcccagcg tctggccgta gttcgggacc
     3181 atgctcacca cctcgtcacc cggccggacg aggtgccaca tcgagacgaa gttcgcctcc
     3241 gagccgccgt tggtgacctc cacgtgctcg atcgtggcgc ccggatacat cgccgcgatg
     3301 cgctcgcgaa gcgcgatcgt gccgttcgac tggttgtagg cgagctcctg ggcgaaaaag
     3361 gcggcgcggt cggcgtcgct gtcgagcagc tcctcgacgc gcatcgggcg gacgccgctc
     3421 tccgagaggt tgtagtccac gaggttctcc cacgtggact gcatccgctc catggcgaag
     3481 agaggaaggc gcatggcggc agtgtaccgg gggcgggaga caggggcaag ggacaaggag
     3541 caagacgccc cgatcagggg agcgcgcagc cagccggccg ttcgccgcac gcggcgcaac
     3601 gggggttcct gggcaggccc agctcccgga acgtcatcgc gagcgcgtcg caaacgatca
     3661 tccggccgac gagcggctcg ccgacgcccg tgatgagctt gatcgcctcc gccgcctgga
     3721 ttgcgccgat gacccccggc agcgcgccaa gaatgccgga gtcggcgcag gaaggctcga
     3781 tctccggggg cggaggctcg ggaaagacgc accgataaca cggaccgccg cgccggccga
     3841 acaccgacat ctgcccctcg aacatgaaga cggccccgaa gacgtccggc ttgtccagga
     3901 gtacgcacgt gtcgttgatc aggtagcgcg tcgggaagtt gtccgagcag tcgaggacga
     3961 tgtcgtatcg ccccaggaga tcaaccgcgt ttccggccgc cagcgcgacc tggtggggct
     4021 cgaccgagac gtggggattg agctcctcca ggcggacgcg ggccggcgcg gtcttcggcg
     4081 cgccgatctc gggcgtgccg tacagcacct ggcgctgcag gttgctcaac gatacccggt
     4141 cgctgtccac gaggccgatc gtgccgaccc ccgccgcggc gaggtagagg ctcgcgggag
     4201 agcccagacc gccggcgccg acgcagagga cgcgggccgc cttcagccgc tgctgccctt
     4261 cgacgccgac gcccggcagc acgagctggc gggcgtaccg ctccagctct tctccggaaa
     4321 agccggaacc agggtgcatg gggaagagga tacaggagat ggggatccag gagactaagg
     4381 aggtgccagg ccaggcgcct cctcgccacc tcaaatcccg acttgaccgg tcttgtattc
     4441 agcggtaatc tgggactgta ggggtcgcat atcggcggga acctatgctg agactgtcga
     4501 agaaagccga ctacgcgctg atcgccatgg cgcacctggc ccagcagcct ggctcggcga
     4561 gcgcccggga gatcgccgag acctacgaca ttcccatgga gctcatggcc aaggtgcttc
     4621 agcggctggt gcgacggggc ctgctcacgt cgcagcaggg cgcgcgggga ggctacaggc
     4681 tgtcccaggc ggcggagagc atctcgctgg cggacgtgat ccaggcgatt gacggccccg
     4741 tctcggtgac ggcgtgttcc tcggacgcgg actgctgcgg ccagtacgag aagtgcaacg
     4801 tgcgtgaccc gctctggcgc atttcgagca tcatcctctc cgctctggcg ggcacgtcgg
     4861 tgtccgagct ggccggcgac ctggccgaca gcgcgcctgg tgccgaccct cgcgagccgg
     4921 cccggctcgc gcaggctgcc attcgagcgt aagcgaaacc catatgctga agttcccgat
     4981 ttacttcgac aaccaggcga cgactccggt ggatccacgg gtcctcgagg cgatgctgcc
     5041 ctacttcacg gagcgcttcg gcaacgcggc gagccgcaac caccggtacg gatgggaggc
     5101 ggaagaggcg gtcgaggccg cgcgcaagca ggtggccgat ctcatcggcg cgaacccgaa
     5161 ggagatcgtc ttcacgagcg gcgccaccga gtcggacaat ctcgccatca ggggcgccgt
     5221 ctacatgtat cgcgagaagg gcgatcacat catcacggcg ctgaccgagc acaaggccgt
     5281 catcgacacc tgcaagcgcc tggagaagga aggcttccgg gtgacgtacc tgccggtgaa
     5341 gccggacggt ctcgtcgacc tcgacgcgct ccgcgacgcc atcacggcga aaacggtcct
     5401 catcacgatc atggcggcca acaacgagat cggggtgctg cagcccgtcg ccgagatcgg
     5461 ggcgatggcg aaggagcgag gcgtcctctt ccataccgac gcggtgcagg cggcgggcaa
     5521 ggtgcccttc gacgtgaacg cgtgcaacgc gcatctggcg tcgctcagcg cgcacaagat
     5581 gtacggcccg aagggcgtgg gcgccctgta cgtccgccgg cggcagccgc gcgtgctgct
     5641 gtcgcccatc atcgacggcg gcgggcacga gcggggcatg cgctcgggca cgctcaacgt
     5701 cccgggcatc gtcggtttcg ggaaggccgc ggagctgtgc cgcctcgaga tggcgcagga
     5761 gagcgccagg atcagggcgc ttcgcgaccg gctgaacgcg aagctgcacg cgggcctcga
     5821 cgagctgcac gtcaacggat cgatggagca ccgcctgccc ggcaacctga acatcagctt
     5881 cgcctacgtc gagggcgagt cgctgctcat gggcatcaac gacgtggcgg tgtcgtcggg
     5941 ctcggcctgc acgtcggcga gcctggagcc gtcctacgtg ctgaaggccc tcggaacggg
     6001 cgacgacctg gcgcactcct cgatccgctt cggcctcggc cgcttcaaca ccgaggagga
     6061 agtggactac gtggccggca aggtcgtggc ggtcgtgcgc aagctgcggg agatgtcgcc
     6121 gctgtacgaa atggcgaagg acggcgtgga cctcgcgaag atggagtggc gggagcacta
     6181 gggacgaagc cgggggccgg aaggcgggcc ccggcgtcgg cgggacgaga ccagccgggc
     6241 cgcggggccc ggcgccgcgg tggagatggg cggatggcgt attcggacaa ggtcgtggat
     6301 cacttcaaca acccgcgcaa cgtgggatcg ctgccgaagc aggacccgac ggtgggcacg
     6361 gggctggtcg gcgcgcccga gtgcggcgac gtgatgaagc tgcaggtccg ggtcaacccg
     6421 gagacgggcg tgatagacga ggccaagttc aagaccttcg gctgcggctc ggcgattgcg
     6481 agctcgagcc tggcgacgga atggctgaag ggcaagacgg tggacgaggc gctgacgatc
     6541 aagaacacgg acatcgtggc cgagctgtcg ctgccgccgg tgaaggtcca ctgctcggtg
     6601 ctggcggagg acgccatcaa ggcggcgatc gcggactacc agcagaagca gcaggggggg
     6661 ccgtcgtcat gatcgccgtc accgagaagg cggcgtcgca catccggaag ctgatggcga
     6721 agcagggcat caccgacggc gggctgcgcg tgggcatcaa gggcggcggc tgctcgggcc
     6781 tcagctacac gttcagctgg gaaaaggaag cgcgcctcgg cgacgaggtg atcgagggcg
     6841 cgggggggac gcgcctcttc gtcgacaaga agagcctcat ctacctgacg gggacgacgc
     6901 tcgagtaaga ctcggcgctg atgggcaaag ggctgcactt cgtcaacccg aacgccaagg
     6961 cctcgtgcgg ctgcggcagc tccttcaccg tctgagcggt tcacgccggc gggcgctcgc
     7021 cggctcgcag gcgcgcccgg accgggtcag cgcggccccc acgccggccc gggcggcatg
     7081 aagccccgcc gggcagccgg cggggcttct cttcgatcct gactatgcag ccggacacca
     7141 aagccgttcc catcgacaag tgcaggcact gcggcagcga ggcgccggtg gacgtccacg
     7201 tctgcccgcg ctgcgaccgg ctcatgacgc tcggccgcct tggcgactac ttcaccttct
     7261 tcgggctccg ccggcgcctc gtcctggacg tccaggagct cgaccagcgc taccgcgcgc
     7321 tcagccggct gttccaccca gactactacc acaacgcgcc ccccgccgag cgcctggcga
     7381 gcctcgagcg cgcgtcgtac ctgaacgacg cgcaccgcac gctgcgcgat ccggccgcgc
     7441 gcctggagta cctgctgaag ctggaagggt tcgcgccgaa gcagtcgcgc gaggcagcct
     7501 cagccgtgcc gcagggcctg ctcgaggagg tgttcgcgct gaacgaggag ctggaggcgg
     7561 tggaagagct gcgggagtcg ggcgcgccgg cggaggccgt cgcggcgcgc ctcgacgagg
     7621 cgcgtcggcc ggtcgaggcg acgctcgagg cccacgagcg gcggctcctc gagctggcgc
     7681 aggcccacga cgccagcctg gagtcgggcg acgccgcggg ccggcgggag acgctcgggc
     7741 tgctggaaac gctgctgctc gagcggacct acctcacgaa cctcctcgcg gccgtcaggc
     7801 gccacgaggc cggcgccggg agcgcgcgaa catgagcagg gtcgtcggca tcgatctcgg
     7861 caccacgaac agcctcgttg ccgtccgccg cgacggcgcc accacggtca tcgccgacga
     7921 gtccggcgac cggctgctcc cgtcggtcgt gtccgtcggg gagtccggcg cgatccacgt
     7981 cggccgcgag gcgcagcggc ggctgctctc cgacgcggcg cgctccgtgt actcggtcaa
     8041 gcggttcatg ggccggggca tcgaggacgc caggggcgag gagcagtggc tgcccttcaa
     8101 gctgggcggc gatcccggga gcgtcatccg cgtccgcctg ggcgagcgga cgttcacgcc
     8161 gcccgagatc tcgagcttca tcctccgcga gctgaagcgc cgcgccgagg ccttcttcgc
     8221 cagggaggcg acatcgaccc ggaggtctcc gcggccgtca tcacggtgcc ggcgtacttc
     8281 aacgacgccc agcgcaccgc cacgcgcgac gccgggcggc tggcgggcct cgaggtcctt
     8341 cgcatcgtca atgagccgac cgccgcgtgc ctggcctacg gcctggacag gaggcggcag
     8401 ggcgtcgtgg ccgtctacga cctgggcggc gggaccttcg acatctcgat cctcaggatc
     8461 gaggacggcg tgttccaggt gctggcgacg aacggcaaca cgcacctcgg cggcgacgac
     8521 ttcgaccgcc tgctcgtcga gatggtggcg cgcgacaccg ggattgatcc ggccgcggcc
     8581 ccggaagccg tgcaggcgat ccggaaggcc gtcatccagg cgaagctgga cctgtccgag
     8641 ttcgaggaga cccgcatcga gatcgacagc ctgcccgggc gggccgggcc gtacagccgg
     8701 cgcctgacgc gcgacgaatt cgaggcaatc atcggcccgg tcgtggccag gacgctggag
     8761 ccgtgccgca aggcgctggc cgacgcgggg ctggatccgg gccaggtgga cgaggcggtc
     8821 ctggtcggcg gctcgacccg cgtgcctctg gtgcgccgcg aagtggagcg gctgttcggc
     8881 cgagcgccgc acacggatct caacccggac gaagtcgtcg cgatcggcgc ggcggtccag
     8941 gcggacatcc tcgtcagcgg ccgccgcgag atgctgctgc tcgacgtgac gccgctctcg
     9001 ctcggcatcg agacgatggg cggagtggtg tccaagatca tcctgcgcaa ctccaccatc
     9061 ccggcgagcg gcggcgagat gttcacgacg ttcgtggaca accagacggc cgtggacatc
     9121 cacatcctgc agggcgagcg cgaaatggcc tccgacaacc ggtcgctggg ccggttcaag
     9181 ctgcgcggca ttccgcccat gcccgcgggc ctgccgcgca tccaggtacg gttccagatc
     9241 gacgcgaacg gcatcctgag cgtctccgcc cgcgagctgc gcacggggat cgcgcagacc
     9301 atcgaggtca agccctccta cgggctcacc gacgaggaag tcgagcggat gctcatcgag
     9361 tcgttcgagc acgcccagga ggacgtcgag gcgcggatgc tcatcgacgc caggaacgag
     9421 gccgagacga tcatcgccgc gacggagaag gcgctcaggc ggccggagtt cggggcgatc
     9481 gccgccgccg acctcgaccc gaaggagctg gtgctcatcc acgcggcgct cgccgatctc
     9541 aaacagtcga tgggcggcgg cgaccgctac gccatccagg tgaaggcggc cgccctcaac
     9601 caggtgacgc ggcacctggc cgaagtgatg atgaaccgga ccgtccaggc ggcgctggcc
     9661 ggccgggccg ccgcggacgt cgccgccgag gggcgctaga tgccgacagt cacgttcatc
     9721 gtcgaggggc aggagcgcac cttcgacgtg gatctcccgt cgcttccgta ctcccaccac
     9781 ggcctgcagg ggtcgctgct ggacatcgcc aagaacctcg acgtgccgct cgagcacgcc
     9841 tgcggcggca gctgcgcgtg cacgacctgc cacgtcatcg tgcgcgaggg agccgggaac
     9901 ctcagcgaca tgcaggatga cgaggccgac cgcctcgaca cggcgtggga cctgaccccg
     9961 cagtcgcgcc tcggctgcca ggccgtcgtg cacggcgacg tcgtgtgcga gatcccgatg
    10021 tacacgcgca actacgtcca ggaagggggc gggatccgcc tcggaagacg cgccggaacc
    10081 tccgaggcgg cgccggccgc cgagccggat tccgccgggg cgaagcgcga gaagctgcca
    10141 tgaagtggac cgacgcggag gagatcggca tcgcgctgga cgagcagtat ccggacgacg
    10201 atccgctggc cgtgcgcttc gccgatctgc gcgggcgcgt gctcggcctc gaggggttcg
    10261 acgatgatcc ccaggcgtcg aacgagccca ggctcgaggc catcctgctg gcctggctgg
    10321 aggagcggaa gtcgcgcctg ggggagtaga atctgcgtgg gggcccggcg cggagcgtga
    10381 caggcggctt cccggcgggc ccggaggcgc caggagaccg atgcgggccg ttgcgatttg
    10441 cgcgcttgcc ctcgcgtgcg gcctctcgcc cgttgcgtcg gacgcccagc cggagccggc
    10501 tgccgcgcca accgaaaagc cccggcccac gcccgcatcg cccgcccagg ccccggaaca
    10561 gtcgcagcca gggacctcga cccaggcgcc ggccgacgcg ccgcggtcac ccgccgaggc
    10621 cgccgtgtcg gcggtgtcgc tcgagcggat caagcgaggg ctcagccagg ccccgccgcc
    10681 gccctcgtcc cgggcgccgc tgaagctcga gtactacgtc gaggtgctgg gtctcgcgcc
    10741 gcccttctcg ctgttcgaga tgtacgacat ggacaccggc cgcatcccgt acagcgcgcc
    10801 cagctactcc gacatgcgga acgtgctgac gcccgcagag ttccgcgcac cggtcgtgtg
    10861 gctcaacagg gcgaccagga gcggcaccag gatgatcctg gatcgctccg cgcagcgctc
    10921 ccgggccagg cgcgcggagg aggatcggcg caaggccctc caggccgagc gcgaccgcca
    10981 ggcgcagctc aagaacagca tcgtcgtcaa gtgactgggg tcagatctgg aattccggcg
    11041 tctggcgcgg atgcggtgcc tgacgacgga attccggatc tgaccccatg caggagtcgg
    11101 gcgaaggcgc gcgccgtcag caccaccctc gggccgggaa ccacgaacgc tcccccgtac
    11161 aggacgtgaa cgcggccgtt cttcacggcc ggcacgcccg ggagcgcctt ccaggcgagc
    11221 cgctcgaggt cggcgtcgcg ctccgtggcg cgctcgtcgt agtgcagctc gatgatgacg
    11281 tccggccgcc tggccaggat tgcctcggtc gccgccatgg tcgactcgcg cggcacgtcc
    11341 ccgaacgcgt tgtcggcgcc cgcgagatcg agca
//
LOCUS       DAOSIF010000036        11335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5675_length_11335_cov_3.769238, whole genome shotgun sequence.
ACCESSION   DAOSIF010000036 DAOSIF010000000
VERSION     DAOSIF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5675_length_11335_cov_3.769238"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLT35_02435"
     CDS             <1..651
                     /locus_tag="PLT35_02435"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOG28113.1"
                     /translation="HQVTSVPAWDFYRLPPGGYERVLEGHDVFVFSDVDAKLFQLAPS
                     FFDREKFGSTILTFPDRIRLTIDAVRAGKGVMFLGGWYSFTGEMGKGGWGRTRLAELL
                     PVRCLETEDLVESTEGFTARPTAAGAPAFAGLDLGAMPPLLGYNKVRPSEDGRVLAEV
                     RETGDPLVAVRRCGAGRSLAFMSDPAPHWACNFVFWERYAEFWLRCLGQVLPDSGR"
     gene            667..1959
                     /locus_tag="PLT35_02440"
     CDS             667..1959
                     /locus_tag="PLT35_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28114.1"
                     /translation="MSGGGRPAAAGLEAGYGERIITPPIGTELAGYGFYLERRAERVL
                     DELHVRSLCVRLGDARVFLISCDLIGLAVDEADRHRRRIADRWGLPPAHVLLACTHTH
                     SGPATKPAIGLGEMDAAYMDRLPGWIDEAVDRSAADLRRCTMRVGEGVVDGIGRNRRG
                     PEAGAADPGLRVARFERPDRRIYLVGYACHPVTAGPTGDVTADWPGAVAACFEEAGDR
                     GLVLQGCSGEIDPVAACRGNGVGASEAVRRTGERLALHARRIAARADPCAPAGVAAAE
                     RRIALPLVAWSGERIAQEARAFVERFGLFPGAPRFASEWERLARASGGRLAERPFVDG
                     VPIQAVGIGPLRILALPGEPFTRIGARIREGAPGAWVAGYANGSVGYLPSREAFADPA
                     DYACWCAPRFTTLVPFAPGLEDRGVREGLAALSAVPVR"
     gene            2083..3294
                     /locus_tag="PLT35_02445"
     CDS             2083..3294
                     /locus_tag="PLT35_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28115.1"
                     /translation="MREPAAAAAAPTTTPVERLVSLDAFRGFVMFLMMAEVLRLAAVA
                     AAVPASAFWRVLAFHQTHAPWEGCSLHDLIQPAFSFMVGVALPYSMASRLARGHSKKA
                     MTAHAVWRAFVLVALGIFLRSIGRDATRFTFEDTLTQIGLGYPVLFLLGFRKMRTAWI
                     AFALILVGYWAAFALYPLPGPGFDWAAAGTTPDWAYNYQGFAAHWNKNTNLAWAFDRW
                     FLNLFPPDGWFRFNGGGYATLSFIPTLGTMFLGLAAGRVMRSGRTARQRMTWLVAAGL
                     IGIAAGWLLDAVGICPSVKRIWTPSWTLFSGGWCFLLLAAFYAVVDLRGWRKPAFPLV
                     VIGMNSIAAYMIAHLFEDFIASSLRTHLGAGFFTAFGAAYEPFVMGAFVLAAYWLILF
                     WMYRRKLFLRI"
     gene            3316..4152
                     /locus_tag="PLT35_02450"
     CDS             3316..4152
                     /locus_tag="PLT35_02450"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOG28116.1"
                     /translation="MRIAPSVLFLAALAVPSGAAAQPAVTPGGVIRLFDGKTLDAFYP
                     WLVDTHREDPLRVFSVVDQVDGAPAVRISGERWGGLVSRESYRDYRLVVEFRWGLLTW
                     GQRKNAARDSGVLIHAQGADGNTGKDFNGAWMRSIEAQIIEGGVGDFILVAGFEADGT
                     RLRPTLEARASQDRDGEWCYDPKGEARDFASGRLNWYGRDADWKDALGFRGREDVESP
                     YGQWTRMEIVAQGDRVTVTVNGTVVNEATRSSLTEGKILIQSEGAEIYFRRIDLEPLA
                     GS"
     gene            complement(4201..5490)
                     /locus_tag="PLT35_02455"
     CDS             complement(4201..5490)
                     /locus_tag="PLT35_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG28117.1"
                     /translation="MNEPRDTKVTRREFVGVAAAATAGFTILKPATVFGTQANSAVSL
                     GLIGAGGRGTAVTRSFLQNSGAVLTAVADIFQDQLDRAKANLDSLNTKLGKPAIARTY
                     KGVGACKELLAGKDIDAVYIATPPFFHPAQLEAALAAGKHVYLEKPVGVDVPGAKKIM
                     ALGEQAKTKKLSLAVGFQIRYASPYVLLEKRIREGQIGTMVSGETHYFASNINRPPFD
                     DKSADERRLRNWIYEKTLSGDIIVEQNIHIIDVTNWLLGGRPVKAFCTNGRAGRTDPG
                     DCSSHYSGVFTYPNDVHISFASTQYGKAAWGVGMQYYGTRGIAEARYDAPVRISGATN
                     WEFPGLGKPEAVDQQAAVTGQFRGALDDADANKQAAFIGSIVTGKLLNEATQGAESTL
                     AGILGRMSAQLGRELTWDEMLKSTEAYDPKINFAQFT"
     gene            5665..6594
                     /locus_tag="PLT35_02460"
     CDS             5665..6594
                     /locus_tag="PLT35_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOG28118.1"
                     /translation="MRTIDRRGFLEMTAAASILAALRPAAASAAAAQAGGGLKKALYV
                     SMLPRELSYLDKFKLAREVGFEGIEIGTIAEPAVAAEIKEAAEKTGLTIHGVMNADHW
                     RYPLSSADPEVVSKSVAGMETSLRNARLWGADTVLLVPAVVNAETSYKDAWTRSQKVI
                     RERILPLAQELKVVVGMENVWNKFLLSPLEMAKYVDEFASPWVKAYLDVGNMLFYGFP
                     DDWIRTLGQRVVKVHVKDFKQERGKSQYAWVNLGNGDVDWPAVRKALADIKYQGWVTT
                     EISGGDAAYLKDVVARLDRIFAGEKPVDTPPVK"
     gene            complement(6700..7824)
                     /locus_tag="PLT35_02465"
     CDS             complement(6700..7824)
                     /locus_tag="PLT35_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOG28119.1"
                     /translation="MSSIRSTGTERRDFLKKAGLGVAAAGTLLTESLSGQSSVNRQLS
                     ESEKLSRLAMTCWPQRHLFKTYGRMGAPDAETAGLRKKYGEITMHDLPKWTKDFFPGI
                     YHLDLWSDVFGDPADTAQYTETTVDRNGKPFTVRRWDPSAPASKKWVEKLASIIANEK
                     FVCQHISNNAPQFLADPDDAKRKDGIRVAKLWLDAASVLGAKTMRCNTGVTGVRIMPE
                     AEAHSTGYPKNEKIVLYLKQCIESFKELADYGATVGVKITIENHWGLCANPMNIRIIM
                     DEVNHPYCECTPDFCNWENEYHLFHGLDAVMPYTHTHVHAKYWDRWKTPEKDWNDVRR
                     SVRILNAHRYTGTIGLEWENGPMDGIEGTKWLMKEVLAAL"
     gene            7988..10222
                     /locus_tag="PLT35_02470"
     CDS             7988..10222
                     /locus_tag="PLT35_02470"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOG28120.1"
                     /translation="MKMMSSTIIWTQTDEAPALVTYSLLPIVRAFTRGTGVAVETRDI
                     SLAGRILAAFPERLDEDRRSPDDLAWLGELTRQPDANIIKLPNISASIPQLKEAIREL
                     QAKGYAVPDYPEAPADPGAREVQARYARVLGSAVNPVLREGNSDRRSPQAVKAFSRSH
                     PHTLGPWTPDSQMHVAHMAGGDFYGSETSVTLTKAAEVRFEFVTPGGAATVLRSPLAL
                     LEGEILDTAVMRVAALRQFYEEQMADAKARGLLLSLHLKATMMKVSDPVIFGHAVSVY
                     FKDVFEKHAAAFKAVGVNPNNGIADLYAKIRALPEAERAAIEADIQAVYAVRPRLAMV
                     DSERGITNLHVPSDIIVDASMPVVIRESGKMWGPDGALHETKALIPDRCYATVYKTII
                     ENCRAHGAFDPATMGSVPNVGLMAQKAEEYGSHDKTFIARGAGTIRVVDAATGETLLS
                     QAVEEGDIFRACQTKDAAVRDWVNLAVRRARATGAPAVFWLDRNRAHDRQVIAKVEGY
                     LKDLDTSGLDIRILAPVDAMTISLERIRKGKDTISVTGNVLRDYLTDLFPILEIGTSA
                     RMLSIVPLLAGGGLFETGAGGSAPKHVQQFQKEGYLRWDSLGEFSALAASLEHIAATA
                     GNPRAALLAETLDQAIGKFLDANRSPARKVGQIDNRGSHFYLALYWAEALAAQSKDAE
                     LQARFASAAARLKESEPAINAELIAAQGRAVDLGGYYRFDPAKAVAAMRPSPTLNAII
                     DAMG"
     gene            complement(10287..10895)
                     /locus_tag="PLT35_02475"
     CDS             complement(10287..10895)
                     /locus_tag="PLT35_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOG28121.1"
                     /translation="MPTTVDALTSLGLGLGLAAACGFRVFVPLLVLSVGAHAGYVPLA
                     DGWQWMGSAPAMIAFACATVFEVLGYCIPWVDHLLDLVATPAAVAAGMVTSASVLADL
                     PPLVRWTAVIIGGGGLAAIVQGATASARVGSTAATGGLANPAVATAELAGSSTASAVA
                     VLFPLFALAGVVLAVALAALAIRRFGARRRQAVSPPACGSSS"
     gene            11006..11251
                     /locus_tag="PLT35_02480"
     CDS             11006..11251
                     /locus_tag="PLT35_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOG28122.1"
                     /translation="MKLVLWLVVGGIVGWLASIVMKTNAQMGLVANVVVGVVGALIGG
                     WIATRLNLGGGALVSFILAVIGASILIWILKLLKILK"
BASE COUNT         1622 a         3889 c         4035 g         1789 t
ORIGIN      
        1 caccaggtca cgtccgtgcc ggcgtgggac ttctacaggc tgccgccggg cgggtacgaa
       61 cgcgtgctgg aggggcacga cgtcttcgtg ttcagcgacg tggatgccaa gctgttccag
      121 ctcgccccct ccttcttcga ccgcgagaag ttcggctcga cgatcctgac gttcccggat
      181 cgcatccggc tgaccatcga cgccgtgcgc gccggcaagg gcgtgatgtt cctcggcggc
      241 tggtattcgt tcaccggcga gatggggaag ggcggatggg gacgcacgcg cctggctgag
      301 ctgctgccgg tgcggtgcct ggagacggag gacctcgtcg agagcaccga aggcttcacg
      361 gcgaggccga ctgcggcggg ggcgccggcg ttcgccggcc tcgacctcgg ggcgatgccg
      421 ccgctcctcg gctacaacaa ggtccggccg agcgaggacg gccgggtgct cgccgaggtg
      481 cgcgagaccg gcgacccgct ggtggccgtc cgccgctgcg gcgccgggcg atccctggcg
      541 ttcatgtcgg acccggcgcc gcactgggcg tgcaacttcg tgttctggga gcgctacgcc
      601 gagttctggc tgcggtgtct cggccaggtg ctgccggact ccggcaggta gacggtggct
      661 cgcgccgtga gtggaggagg caggcctgcg gccgccgggc tggaggcggg ctacggcgag
      721 cggatcatca cgccgccgat tggcaccgaa ctggccggct acgggttcta cctcgagcgt
      781 cgggccgagc gggtgctcga cgagctgcac gtccgctcgc tgtgcgtccg cctcggagat
      841 gcccgggtct tcctgatctc gtgcgacctt atcgggctcg cggtggacga ggccgaccgg
      901 caccgccgcc ggatcgccga tcggtggggc ctgcccccgg cccacgtgct gctcgcctgc
      961 acccacaccc actccggacc ggccacgaag cccgcgatcg gcctgggcga gatggatgcc
     1021 gcctacatgg accggctgcc cgggtggatc gacgaggccg ttgatcggtc cgccgccgat
     1081 cttcgcaggt gcaccatgcg ggtaggggag ggcgtcgttg acggcatcgg ccgcaaccgc
     1141 cgcggtccgg aggccggcgc cgcggaccca gggctgcgcg tggcgcggtt cgaacggccg
     1201 gatcgcagga tctacctggt cggctacgcc tgtcatccgg tgacggcagg gccgacgggc
     1261 gatgtcacgg ccgactggcc tggcgcggtg gcggcctgct tcgaagaggc cggcgaccgc
     1321 ggactcgtgc tccaggggtg cagcggcgag atcgatcccg tggctgcatg ccgcgggaac
     1381 ggggttggcg cgtccgaggc tgtgcggcgg acgggggagc ggctggcgct gcacgcgcgc
     1441 cggattgccg cccgcgcgga cccgtgcgcc cccgccgggg tggcggccgc cgagcggcgc
     1501 atcgcgctgc cgctcgtcgc ctggagcggc gagcggatcg cgcaagaggc ccgggcgttt
     1561 gtcgagcggt tcggcctgtt ccccggcgcg ccgcggttcg cctcggagtg ggagcgcctc
     1621 gcgcgcgcct ccggcggccg gctggccgag cggccgttcg tcgacggcgt gcctatccag
     1681 gccgtcggga tcggccccct gcggatcctg gcgcttcccg gggagccgtt cacccgcatc
     1741 ggcgcccgga tccgggaggg cgccccaggg gcgtgggttg ccggctacgc caacggcagc
     1801 gtcggctacc tgccgagccg cgaggcgttc gccgacccgg ccgactacgc ctgctggtgc
     1861 gcgccccgtt tcacgacgct cgtcccgttc gcgccaggcc tggaggaccg cggggtccgg
     1921 gagggcctcg ccgcgctgtc ggccgtgccg gtgcgctagg cggccggcgc cgagcctgcg
     1981 ccggccgccg ggaggccggt tcccgagtcg ctcttccttg cgcctgccct cgcgctccca
     2041 aagaacgtgc gcggcgcggc gcggcacggt atcatcccgg gcatgcgtga acccgctgcc
     2101 gctgctgcgg ccccgacgac gactccagtc gagcgtctcg tgtcgctcga cgccttccgc
     2161 ggcttcgtga tgttcctgat gatggccgag gtgctccgcc tggcggcggt ggccgccgcc
     2221 gtgcccgcaa gcgcgttctg gagggtcctc gcgttccacc agacccacgc gccgtgggag
     2281 ggctgttcgc tgcacgatct catccagccg gcgttctcgt tcatggtggg cgtggccctg
     2341 ccgtactcga tggcgagccg cctcgcgcgg ggccactcga agaaagccat gaccgcgcac
     2401 gccgtctggc gcgcgttcgt gctcgtggcg ctgggcatct tcctgcgatc catcggccgg
     2461 gacgccacgc ggttcacctt cgaggatacg ctcacgcaga tcggcctggg ctatcccgtg
     2521 ctgttcctgc tgggcttccg caagatgcgg accgcctgga ttgcgttcgc cctcatcctg
     2581 gtcggctact gggcggcgtt cgcgctgtac ccgctgcccg gtcccggctt cgactgggcg
     2641 gctgccggca cgacgccaga ctgggcgtac aactaccagg ggttcgcggc gcactggaac
     2701 aagaacacga atctcgcgtg ggccttcgac cgctggttcc tcaatctgtt cccgccggac
     2761 ggctggttca ggttcaacgg cggcggctac gcgacgctga gcttcatccc gacgctcggc
     2821 acgatgttcc tgggactggc cgccgggcgc gtcatgcgca gcggccggac ggcccggcag
     2881 cggatgacgt ggctcgtggc ggcgggcctc atcgggatcg ccgccggctg gctgctggac
     2941 gcggtcggga tttgcccgtc ggtcaaacgc atctggacac cgagctggac gctgttcagc
     3001 ggcggctggt gcttcctgct cctggccgcg ttctacgcgg tggtggacct gcgcggctgg
     3061 cgcaagccgg cgttcccgct ggtcgtcatc gggatgaact cgattgcggc ctacatgatc
     3121 gcgcacctct tcgaggactt catcgcgtcc tccctgcgca cccatctcgg cgcgggcttc
     3181 ttcacggcct tcggcgccgc gtacgagccg ttcgtgatgg gcgcgttcgt cctggcggcg
     3241 tactggctga ttctgttctg gatgtaccgc cgcaagctgt tcctgagaat ctgaccctga
     3301 tcggtggagg tcccgatgcg catcgcgccg tccgtcctgt tccttgccgc gctcgccgtg
     3361 ccctctggcg ccgccgccca gccggccgtc acgccgggcg gcgtgattcg cctcttcgac
     3421 gggaagacgc tcgacgcgtt ctacccgtgg ctcgtggata cgcaccggga ggatccgctg
     3481 cgggtgttca gcgtcgtgga tcaggtggac ggggcgcccg ccgtccgcat cagcggggag
     3541 cgctggggcg gcctggtgtc ccgggagtcc taccgggact accggttggt cgtggagttc
     3601 cggtgggggc tgctgacctg gggccagcgg aagaacgcgg cccgcgacag cggcgtcctc
     3661 atccacgcgc agggggcgga cggcaatacc ggaaaggact tcaacggcgc gtggatgcga
     3721 tccatcgagg cccagatcat cgagggcggc gtcggcgatt tcatcctggt cgccggtttc
     3781 gaggccgacg gcacccggct gaggccgacg ctggaggcac gggcgtcgca ggaccgcgac
     3841 ggcgagtggt gctacgaccc caagggggag gcgcgcgatt tcgcgagcgg ccgcctcaac
     3901 tggtacggcc gggacgccga ctggaaggac gcgctggggt ttcgcggccg ggaggacgtt
     3961 gagagcccgt acgggcagtg gacacgcatg gagatcgtgg cgcagggcga ccgggtgacc
     4021 gtgacggtga acggcacggt cgtgaacgag gcgacccggt cgagcctgac ggaggggaag
     4081 atcctgatcc agtccgaggg cgcggagatc tacttccgga ggatcgatct cgagccgctg
     4141 gcgggatcct gacggggcgg ggcccgccgc cgactccggc cgcgggcccc tgcagcatcc
     4201 ttaggtgaac tgcgcgaagt tgatcttcgg gtcgtacgcc tcggtcgact tcagcatctc
     4261 gtcccaggtc aactcgcggc cgagctgcgc cgacatgcgg ccgaggatgc ccgcgagggt
     4321 ggactcggcg ccctgcgtgg cctcgttgag cagcttgccg gtgacgatgc tgccgatgaa
     4381 cgccgcctgc ttgttcgcgt cggcgtcgtc gagcgcgccg cggaactgcc ccgtgaccgc
     4441 ggcctgctga tcgacggcct cgggcttgcc gagcccgggg aactcccagt tcgtggcgcc
     4501 cgagatgcgc accggcgcgt cgtagcgcgc ctcggcgatg ccccgcgtgc cgtagtactg
     4561 catgccgacg ccccacgccg ccttgccgta ctgggtcgag gcgaagctga tgtgcacgtc
     4621 gttcggatac gtgaacacgc cgctgtagtg gctcgagcag tcgcccggat ccgtgcgccc
     4681 ggcgcggccg ttcgtgcaga acgccttgac ggggcgtccg cccagcagcc agttggtgac
     4741 gtcgatgatg tggatgttct gttcgacgat gatgtcgccc gacagcgtct tttcgtagat
     4801 ccagttgcgg aggcggcgct cgtcggccga cttgtcgtcg aagggcggcc ggttgatgtt
     4861 ggacgcgaag tagtgggtct cgcccgacac catcgtgccg atctgcccct cgcggatgcg
     4921 cttctcgagc agcacgtagg gcgacgcgta ccggatctgg aaccccacgg cgaggctcag
     4981 cttcttggtc ttggcctgtt cgccgagcgc catgatcttc ttggccccgg gcacgtccac
     5041 gccgaccggc ttctccaggt acacgtgctt cccggcggcg agcgccgcct cgagctgcgc
     5101 cgggtggaag aacggcggcg tggcgatgta caccgcgtcg atgtccttgc cggccagcag
     5161 ttccttgcag gcgccgacgc ccttgtaggt ccgcgcgatc gccggcttgc caagcttcgt
     5221 gttgagggag tcgaggttcg ccttggcccg atccagctgg tcctggaaga tgtcggccac
     5281 cgccgtgagc acggcgccgc tgttctggag gaacgagcgc gtcacggccg tgccgcgccc
     5341 tccggcgccg atcaggccga ggctcacggc ggagttcgcc tgcgtgccga aaaccgtggc
     5401 aggcttcagg atggtgaatc ccgcggtcgc ggcggcggcc acgcccacga attcgcggcg
     5461 cgtgaccttg gtgtctctgg gttcgttcat cgatctccct ccttcaggtg atcccggtct
     5521 ccggcggagg ctggtccgac gcgtcaagca accctcgaag accgggcaag cggggtgctc
     5581 ccgtcagatt ataccgggaa cgcgggcgcc gggggcttga gtcgcgcgcg ccaacggagt
     5641 accctccgct cgagggaggt ggccatgaga acgatcgatc ggcgcgggtt cctggagatg
     5701 acggcggcag cctcgatcct ggcggcgctc cggcccgctg cggcctctgc ggcggcggcc
     5761 caggcgggcg gcgggttgaa gaaggccctc tacgtcagca tgctgccgag ggaactgtcc
     5821 tatctcgaca agttcaagct ggcgcgcgag gtggggttcg agggcatcga gatcggcacg
     5881 attgccgaac cggccgtcgc ggcggagatc aaggaggccg ccgagaagac cggcctgacg
     5941 atccacggcg tgatgaacgc cgaccattgg cggtaccccc tgtcgagcgc cgaccccgag
     6001 gtggtgtcga agagcgtggc gggtatggag acgtccctcc gcaacgccag gctgtggggc
     6061 gctgacacgg tgctcctggt gccggcggtg gtgaacgccg agacctccta caaggacgcc
     6121 tggacgcgct cgcagaaggt gatcagggag cgcatcctgc ccctcgcgca ggagctgaag
     6181 gtcgtcgtcg ggatggagaa cgtctggaac aagttcctgc tcagcccgct cgagatggcc
     6241 aagtacgtgg atgagttcgc gtcgccctgg gtgaaggcgt acctcgacgt gggcaacatg
     6301 ctcttctacg ggttcccgga cgactggatc cgcacgctcg gccagcgcgt cgtgaaagtc
     6361 cacgtgaagg acttcaaaca ggagcgcggc aagagccagt acgcctgggt gaacctgggc
     6421 aacggcgacg tcgactggcc ggccgtccgc aaggccctcg ccgacatcaa gtaccagggc
     6481 tgggtcacga ccgagatcag cgggggggac gcggcctacc tgaaggacgt ggtggcgcgc
     6541 ctcgatcgca tcttcgcggg ggagaaaccg gtcgacacgc cgccggtgaa gtaggggcag
     6601 gggacaagcg gtggaagggg ctcggcgcta ggggctggtc ggccgcggcg cgtgccgcac
     6661 cagactagcg cctagcgcct cgtccctatc gcctcccgcc tacagcgccg cgaggacctc
     6721 cttcatcagc cacttcgtcc cctcgatgcc gtccatcggc ccgttctccc actcgaggcc
     6781 gatcgtcccc gtgtagcggt gcgcgttcag gatgcgcacg ctgcgccgca cgtcgttcca
     6841 gtccttctcg ggcgtcttcc agcggtccca gtacttggcg tgcacgtggg tgtgcgtgta
     6901 cggcatcacc gcgtcgaggc cgtggaacag gtggtactcg ttctcccagt tgcagaagtc
     6961 cggcgtgcac tcgcagtacg ggtggttcac ctcgtccatg atgatccgga tgttcatggg
     7021 gttggcgcag aggccccagt ggttctcgat ggtgatcttc acaccgaccg tcgcgccgta
     7081 gtcagccagc tccttgaacg actcgatgca ctgcttcagg tacagcacga tcttctcgtt
     7141 cttcgggtac ccggtcgagt gggcctcggc ctcgggcatg atccgcacgc ccgtgacgcc
     7201 ggtgttgcag cgcatcgtct tggccccgag caccgacgcg gcgtccagcc agagcttcgc
     7261 gacgcggatg ccgtccttgc gcttcgcatc gtcgggatcc gccaggaact gcggcgcgtt
     7321 gttggagatg tgctggcaca cgaacttctc gttcgcgatg atggacgcca gcttctcgac
     7381 ccacttcttg gaagcgggag ccgacggatc ccagcgccgg accgtgaacg gcttgccgtt
     7441 ccggtccacg gtcgtttcgg tgtactgcgc cgtgtcggcc gggtcgccga agacgtcgga
     7501 ccacaaatcc aggtggtaaa tgcccgggaa gaagtccttc gtccacttgg gcaggtcgtg
     7561 catcgtgatc tcgccgtact tcttccgcag cccggccgtc tcggcgtccg gcgcgcccat
     7621 ccggccgtac gtcttgaaca agtggcgctg cggccagcac gtcatcgcga ggcgcgacag
     7681 cttctccgac tccgagagct gccggttgac ggagctctgc ccggacaggg attcggtgag
     7741 cagagtcccc gcggcggcga cgccaaggcc ggccttcttc aggaagtcgc ggcgctcggt
     7801 gccggtcgat cgaattgaac tcatgggtcc tcctgagcac tcgggggccg tcccgggcat
     7861 cgtacactac gacgcggcct acctgccagc cggaccgcgg acgcgcggct cgccggacgg
     7921 cactatactg gcgaggcgcg cgtgacccgg cgcgccgggg gagggccctc gaccgggagg
     7981 atcaggcatg aagatgatgt cgtcgacgat catctggacc cagaccgacg aagcgccggc
     8041 gctcgtcacc tattcgctgc tgcctatcgt cagggccttc accaggggga ccggcgtcgc
     8101 cgtcgagacg cgcgacatct cgctcgcggg gcgcatcctc gccgcctttc ccgaacgcct
     8161 ggacgaggac cggcgcagcc cggacgacct ggcgtggctc ggcgagctca cccggcagcc
     8221 cgacgccaac atcatcaagc tgccgaacat ttcggcctcc atcccgcagc tcaaggaagc
     8281 catcagggaa ctgcaggcga aggggtacgc cgtgcctgat taccccgagg ccccggccga
     8341 ccctggcgcc cgggaggtcc aggcccgcta cgccagggtg ctcggcagcg ccgtgaaccc
     8401 ggtcctgcgc gaggggaact cggaccggcg gtcgccccag gcggtcaagg cgttctcccg
     8461 gtcgcacccg cacacgctcg ggccgtggac gccggactcg cagatgcacg tcgcgcacat
     8521 ggccggcggc gacttctacg gcagcgagac gtcggtcacg ctgacaaagg ctgccgaggt
     8581 ccgcttcgag ttcgtcacgc ccggcggggc cgccaccgtc ctccgctcgc cgctcgcctt
     8641 gctcgagggc gaaatcctcg acaccgccgt catgcgcgtc gcggcgctgc gccagttcta
     8701 tgaggagcag atggccgacg cgaaggcgcg cggcctgctg ctgtcgctgc acctgaaggc
     8761 gacgatgatg aaggtgtccg acccggtgat attcggccac gccgtcagcg tctacttcaa
     8821 ggacgtcttc gagaagcacg cggcggcgtt caaggcggtg ggcgtcaacc cgaacaacgg
     8881 gatcgccgac ctctacgcga agatccgcgc cctcccggag gccgagcgcg ccgccatcga
     8941 ggccgacatc caggccgtct acgccgtccg cccgcgcctc gcgatggtgg actcggaacg
     9001 gggcatcacg aacctccacg tgccgagcga catcatcgtg gacgcgtcga tgccggtcgt
     9061 catccgcgag tccgggaaaa tgtggggccc cgacggcgcg ctgcacgaga ccaaggcgct
     9121 gatcccggac cggtgctacg ccacggtcta caagaccatc atcgagaact gccgggcgca
     9181 cggcgcgttc gaccccgcca cgatgggctc cgtcccgaac gtcggcctca tggcccagaa
     9241 agcggaggag tacggctcgc acgacaagac gttcatcgcc cggggcgccg gcacgatccg
     9301 cgttgtcgac gccgcaaccg gcgagacgct gctgtcccag gcggtcgaag agggagacat
     9361 cttccgggcc tgccagacga aggatgccgc cgtccgcgac tgggtcaacc tggccgtccg
     9421 gcgcgctcgg gccaccggcg caccggccgt cttctggctc gaccgcaacc gcgcgcacga
     9481 ccggcaggtc atcgcgaagg tcgaggggta cttgaaggac ctcgatacct cggggctcga
     9541 catccgcatc ctggcgcccg tggacgcgat gacgatttcc ctcgagcgca tccgcaaggg
     9601 caaggacacg atttcggtga ccgggaacgt gctgcgcgac tacctgacgg acctcttccc
     9661 gatcctggag atcggcacgt ccgcgaggat gctgtcgatt gtgccgctgc tcgccggggg
     9721 cggtctgttc gaaacgggcg ccggcgggtc ggcgccgaag cacgtccagc agttccagaa
     9781 ggaagggtac ctgcgctggg attccctcgg cgagttctcc gcgctggccg cgtcgctcga
     9841 gcacatcgcc gcgacggccg gaaaccccag ggcggccctc ctcgccgaga cgctggacca
     9901 ggcgatcggc aagttcctgg acgccaacag gtcgccggcc cgcaaggtcg ggcagatcga
     9961 caaccgcggc agtcacttct acctggctct gtactgggcg gaggcgctcg cggcacagtc
    10021 gaaggacgcc gagctccagg cccgctttgc gtccgcggca gcgcgcctga aggagtccga
    10081 accggccatc aatgccgaat tgatcgcggc gcaggggcgg gcggtggacc tgggcggcta
    10141 ctatcggttc gaccccgcga aggccgtcgc cgcgatgcgc cccagcccga cgctgaacgc
    10201 gatcatcgac gccatgggct gagtcaccgg cgggtggacc cgcggcggct gcgcccggac
    10261 gcgagcgcag aagccccgcg gtcgcgtcag gaggacgaac cgcacgcggg cggactgacg
    10321 gcctggcggc gcctggcccc gaaccgccga atcgccaggg cggcgagcgc gaccgccagg
    10381 acgacgccgg ccagcgcgaa gagcgggaac agcacggcga cggcgctggc ggtcgacgag
    10441 ccggccagct cggccgtcgc cacagcggga ttggccagac cgccggtcgc cgccgtcgat
    10501 cccacccgcg ccgacgcagt cgcgccctga acgatcgcgg cgaggcctcc accgccgatg
    10561 atcacggccg tccagcgcac cagcggcggc aggtcggcca gcaccgacgc gctggtcacc
    10621 atccccgccg ctacggcagc cggcgtcgcg acgaggtcca gcaggtgatc cacccagggg
    10681 atgcagtagc cgagcacctc gaacaccgtc gcacacgcga acgcaatcat ggccggcgcc
    10741 gaccccatcc actgccaccc gtccgcgagc ggcacgtagc cggcgtgcgc gcccacgctg
    10801 aggacgagca gcggaacgaa gacccggaac ccgcacgccg cggcgaggcc cagcccgagt
    10861 ccgaggctgg tgagcgcgtc gaccgtcgtg ggcatgccgc tatgatacgc ccgttcggcg
    10921 gttgcttgaa tcgacggggc ggatcgctca tcctagctgc ggtcgcccgc aggccgctct
    10981 ccggcggcgc gggcaggggg gcgccatgaa gctcgttctc tggctggttg tcggcgggat
    11041 cgtcggctgg ctcgccagca tcgtcatgaa gacgaacgcc cagatgggcc ttgtcgccaa
    11101 cgtcgtcgtc ggcgtcgtcg gggcgctcat cggcggctgg atcgcgaccc ggctgaacct
    11161 gggcggcggc gccctggtca gcttcatcct cgccgtgatt ggcgcctcca tcctgatctg
    11221 gatcctgaaa ctcctgaaga tcctcaagta gtcgggtgcg acgcccggcc cgggcgcgtc
    11281 accccccgag cggcggaatc gtcacccagc gccgaagtcg ccaggattcg agcgc
//
LOCUS       DAOSIF010000037        11276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5708_length_11276_cov_4.078335, whole genome shotgun sequence.
ACCESSION   DAOSIF010000037 DAOSIF010000000
VERSION     DAOSIF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_11276_cov_4.078335"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLT35_02485"
     CDS             complement(<1..543)
                     /locus_tag="PLT35_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOG28123.1"
                     /translation="MLVCDGAMGTLLYSKGVFINRCFDGLNLTQPAIVAEVHTEYVRA
                     GADVIETNTFGANRIKLASFGLGGQVRAVNTEGAKIARGAAKGRAYVAGAIGPLGIRI
                     EPWGRLGVDEAQEYFLEQAQALADAGVDLFIVETFRDLNEIRAAVAAVMSVCGLPVVA
                     QMTTEEDGNSLDGTPPEEFAP"
     gene            complement(588..1025)
                     /locus_tag="PLT35_02490"
     CDS             complement(588..1025)
                     /locus_tag="PLT35_02490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOG28124.1"
                     /translation="MIRPLVTGFATTLRHFFRKPITVNYPDQKIPVFPKYRGKQVLMR
                     DEHGLEKCVACGLCAVACPSDAIYLEAAENDGSVQAGPRYARVYQINKTRCIFCGYCE
                     EACPVAAIFMGKDYELAVYSKADFIWDKQDLLVPAAAAAAPRR"
     gene            1265..1474
                     /locus_tag="PLT35_02495"
     CDS             1265..1474
                     /locus_tag="PLT35_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28125.1"
                     /translation="MTPILALAADPGEPLGCNASTPRWEAGGLTTGRISATSNCRTLD
                     ETQARPEPDAVWRACRPHPASIGPV"
     gene            1577..2248
                     /gene="lepB"
                     /locus_tag="PLT35_02500"
     CDS             1577..2248
                     /gene="lepB"
                     /locus_tag="PLT35_02500"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG28126.1"
                     /translation="MLEAPAVSDQVGTEDAAAAPPEAESARPTVPPPPLRAEAEPPAA
                     ARPRPSHWAVVRAELVAWIKTLGSAAVYATLIVTFGFQVARVEGLSMAPTLEDQDRLI
                     VNKAVYRLTSPRRGDIVMLYYPVNPDKSFVKRVIAEEGDTVRITDGRVFVNDLPVADD
                     YVPPEYRSHDDWGPQVIPDGYYFVMGDHRNNSSDSRHWGMVPKRYIIGRVQARWWPIP
                     EARLF"
     gene            complement(2261..4120)
                     /locus_tag="PLT35_02505"
     CDS             complement(2261..4120)
                     /locus_tag="PLT35_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOG28127.1"
                     /translation="MLQPKPGHAAVRTFAELPFYLAERYDRPVLVRRCLADGFHEFST
                     RAFVSQTRALSLSLQEMGIGRGDRVGLICESRPEWSMADLAILASGAVNVPVYPTLAA
                     SQTQYILADAGVKAVVVSDDVQLAKLLGVSASLPALAAIIVIAPSQPLPDAGPGRPAV
                     HAMPDAVRRGEALLAADPGLAAQHEARARSVEPGDIATIIYTSGTTGPPKGVVLTHAN
                     MLSNLWAANTAFGIDERDVPLSFLPLSHVFERVVLYLFLLVGATVTFAENLQTIARDL
                     QRVRPTVMTGVPRVFEKFHHAVLDAVAAGPPLNRALFRWAMRVSTAIVRSRFEGRPAP
                     LRAVIQRPAADALVLRKIRRRLGGRLRTAVSGSAQLARTTEEFLFAVGVPIYNCYGLT
                     ECSPGVTVNPRHAPRPGTVGVAIPDVDVRIADDGEILVRGPNIMQGYYNRPGETADAI
                     RGGWLHTGDIGRLDADGYLTITDRKKDIIVTSGGKNIAPAPIEQRLTSSPLVAEAVLV
                     GEARSYPAALIVPNFALLEERLRAQGAASGTKAQLVERPDVLALYADLLDAVNVDLAQ
                     FEKIKRFALLPRELSVEGGELTPTMKVRRKVVEQEWADVVERLYAHQPSPARA"
     gene            complement(4214..5188)
                     /locus_tag="PLT35_02510"
     CDS             complement(4214..5188)
                     /locus_tag="PLT35_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOG28128.1"
                     /translation="MDVLVAIFSEQPAWNIPAEAVESLRRRFPEHAFLQAGDEATMID
                     LIRTAEAAFTSRLTPAALAAAPKLRWVHSPAAGVGGMLFPAMRASRVVLTNSRGMSAA
                     AVAEHALLLMLASARRFPEAVLAQAERKWIGDDLAGLPTLRGRTLAIVGLGAIGRALA
                     RAAAALGMTVVGTRKTAAGAPPEGVTDAFGPEGLPTVLACADFVVLAAPLTDETRGMI
                     DAPQLRLMRPSAWLINVARGKLVNETALVDALRRGGIAGAALDVFDREPLDASSPLWS
                     LPNVLVTPHVGGFRSGYWEAAVDLFAENLGRFAKNERLRNTVDKAAGY"
     gene            complement(5191..5700)
                     /gene="moaC"
                     /locus_tag="PLT35_02515"
     CDS             complement(5191..5700)
                     /gene="moaC"
                     /locus_tag="PLT35_02515"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOG28129.1"
                     /translation="MPRGIDAQEPPTGARLTHTDAKGRARMVDVSAKPATVREAVARG
                     FVRLAPSTLRLVRSGRSAKGHPLEAARLAGILAAKKTADLIPLCHPIALTHCDVTLKL
                     GRRGCEIEARTRTTGPTGVEMEALTAVAVAALTIYDMVKAVDRGAVIGEIRLMEKRGG
                     RSGAWTRRA"
     gene            complement(5702..7204)
                     /locus_tag="PLT35_02520"
     CDS             complement(5702..7204)
                     /locus_tag="PLT35_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28130.1"
                     /translation="MEMGAATAPAAPGPGAASDRDRTLRNVGEVVAQRGLHILAAALF
                     AAFVPRLMGPDRFGRFALLLSVSLWFALLSGFGAVSVMSRAVPRLLAEGQTHAVRRLT
                     TSLMALRALTGPLAAAGCFAVLAFVLREPDRVASALVALAVVGRTINNVAFSLFLGLN
                     DAGRWGRGELLRRWLTLALAPAGFVAGGLRGACAGVAAAEAVVLGLGLWWVRAYIGRD
                     ALDLSRRYLSPFLRAGSGFGVAAVLAAITQRSGEVIVRLATGRYEEVGYLGAAYSIYL
                     TVAFVLWHATFACLPFIVGLLGQGRRAEVSAWLERLLTYGAIAAAICVAGAVVAGPVA
                     VPWVLGRSYAPVAASLVPLMVAMLFHAAGDVGRLLALAVDPPRIVTAAAALELAAFWG
                     IGLPLAARAGSVGAAMAAVAAAALYAAFLTRHARRSVRYSLAAAGRAVALAVVFVPPA
                     LARGAWPLNAVLLAVALIAYAILLVSCRVVTAGELAEVRRLLLGRAAARR"
     gene            7362..9428
                     /locus_tag="PLT35_02525"
     CDS             7362..9428
                     /locus_tag="PLT35_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOG28131.1"
                     /translation="MPFVPDAAEQREFTLRALVLGLVMCVILGAANAYLGLRAGMTIA
                     ATYPAAVIGMAVLRLFRNSTILEENFARTVGSIGESVAAGAIFTIPAFVILSLWKFDA
                     AHLWQEYLVSSALMILGGLLGIMFVTILRRVMVEDPSLQFPESVAASEIHKAGQKGSE
                     AALQLFRAMGVGALVRIVSSAGFGLFRDQNEFTLPVGRLRESFVRLGLTDESAKITAG
                     GVTTFSAPDVTPAYLGVGYIIGPELGALNFAGGLLAWGLFVPLLVFFLGPGLIDQYLA
                     ASGGNEEAAWGAMVVALWKYIVRPIAVGGMLVGATFTLYKMRKNLIGGFKRGVADVKK
                     SATAQAATKRTERDLSLKVVGLGILAIFVLMIALYSYFTGAIGTAVTAAVVMIVTGFF
                     FAAVSGNLVGLIGSSNNPLSGLTLATTIIAALTMVIIGATGAQGVVAVLGVAAVGCVS
                     AAVAGEMLQDLKVGHILGGTPWKMQIGDIFGVVVAGLVMFFPLYMMHNSDLAQFGASG
                     GFGGRTYSAPQAGLMAALSQGIVGGEMAWPLVFVGIAMGISLILIRVRSPMLFSVGMY
                     LPLGTTFAIFCGGVIRGVVDKIRDHRGYNAAQKARVENAGVLAASGLIAGEALVGLLI
                     AGVVYARASHAFWTWRDLFQSRALESLVPWMSIVAAAVLVWYLIAVPLRKAGDADEPA
                     PPTAVM"
     gene            complement(9566..>11276)
                     /locus_tag="PLT35_02530"
     CDS             complement(9566..>11276)
                     /locus_tag="PLT35_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG28132.1"
                     /translation="VVGPEDLLAVMTPIMAASDITFTRRTDLVAEMLDSAGMWGQRDE
                     RMKKDPVDQLYEQCYPPAPGTGRATSEVAEAMIQRRREKVTLDAIEELVRYLGGLREE
                     RKAILLVSEGWMLYRPARPLAESGTPRMPGVFVGPDGKLRNTDARNMAGANTAQCDQD
                     RVALSMIDNRQRYLNILNLANRANASFYPIEPRGLAVFDNDIGPNPPPLPATDVAMVR
                     TRHEALRTAALNTDGIAVMDSNDIDSGLKRVASDLSSYYLLGYTSTNSKFDGKYRAIR
                     VRVARPGIEVRARRGYLALTEAEAATMTAAAPAPSAETVALRSALASLDAIRPDRAFT
                     LSASWGWWMPAGEPLKGQPRGAEPAVWIVGAIDPKARGAEAWTRGGEAEVTVAAGGGA
                     VLASYTVPIPAESARFESRFPRSAEDVWLDPGAYTIRVRAQPAGGGLPTTDTVRLTVP
                     EAPGAGRFALGQPVCVRRGAGVSAAESSVPDRRLRRTERLIVLASASSAPDELSAELL
                     DRRGAPMTLPVETSTMSRDFVQWIRAELALAPLAPGDYIVRLSAKKGGEQAVTLAAFR
                     VVP"
BASE COUNT         1599 a         4094 c         3952 g         1631 t
ORIGIN      
        1 cggcgcgaac tcctccggcg gcgtgccgtc gaggctgttg ccgtcctcct cggtcgtcat
       61 ctgcgcgacc accggcagcc cgcacacgct catcaccgcc gcgaccgccg cgcggatctc
      121 gttcaggtcc cggaaggtct cgacgatgaa caggtccacg cccgcgtccg ccagggcctg
      181 agcctgctcg aggaagtatt cctgcgcctc gtccacgccc agccggcccc agggctcgat
      241 gcggatgccg agcggtccga tcgccccagc cacatacgcc cggccctttg ccgcgccgcg
      301 ggcgatcttc gcgccctcgg tgttgacggc gcgtacctgc ccgccgagcc cgaagctcgc
      361 cagcttgatc cggttcgcgc cgaacgtgtt cgtctcgatg acgtccgcgc cggcccgcac
      421 atactcggtg tggacctcgg ccacgatggc gggctgggtc agattcaggc cgtcgaagca
      481 gcggttgatg aagacgccct tcgagtacag gagcgtcccc atagcgccgt cgcagaccag
      541 cacgcgctcg tccagggcgt caagaaaagg agggagcggg gcggggctca ccgtcggggg
      601 gcggccgcgg cggccgccgg aacgagcaga tcctgcttgt cccaaatgaa gtccgccttg
      661 ctgtagacgg ccagttcgta gtccttgccc atgaagatgg ccgcgaccgg gcaggcttcc
      721 tcgcagtacc cgcagaagat gcaccgcgtc ttgttgatct ggtacaccct cgcgtagcgc
      781 ggcccggcct gcacggaccc gtcgttctcc gcagcctcga ggtagatggc gtcagacggg
      841 caggccacgg cgcacaggcc gcaggccacg cacttctcca ggccgtgctc gtcccgcatc
      901 agcacctgct tgccgcggta cttcgggaag acggggatct tctgatcggg gtagttgacg
      961 gtgatgggct tcctgaaaaa atgcctcagc gtcgtcgcaa agcccgtgac caagggacga
     1021 atcatccgcc gcgcctcccc gccgctccat cataacacgc tcatcttcag cggtttgaca
     1081 gccctccgag acggccacca tcgtcgaacc cggagcgcgg cggcgcggcc cgctccaccg
     1141 ggcgcgcgcg tgtcggccgc atccgggttc agcatcctgc ggatgtccct tccggccggc
     1201 tgtcatgccg gcgtcctgtc actgttgccg cggctcccgg atccgcgagc agggcagccc
     1261 ggccatgacg cccatcctcg ccctcgccgc cgacccgggc gagcccctcg gctgcaacgc
     1321 ctcgacgccc cggtgggagg ctggagggtt gacgaccggg cggatctccg cgacgtcgaa
     1381 ctgtcgtaca ctggacgaga cccaggcccg gccggagccc gacgccgtct ggcgcgcctg
     1441 ccggccgcac ccggccagca tcgggccagt gtaaggagat cgcgccttgg ttcagcagga
     1501 acgacacgct caggatgaag ggctcgaggc tcacggtgag ccagctcggg gggccgcagg
     1561 cgaagcggct ccgctcgtgc tcgaagcgcc ggccgtttcc gatcaggtcg gcaccgagga
     1621 cgcggccgcg gcgccgccag aggcggagtc cgcccgcccg acggtgccgc cgccacccct
     1681 ccgggccgag gccgagccgc ctgccgccgc caggccccgc cccagtcact gggccgtggt
     1741 gcgcgcggag ctggtggcgt ggatcaagac gctgggctcc gccgccgtct acgcgacgct
     1801 catcgtgacc ttcgggttcc aagtcgcccg cgtcgagggg ctgagcatgg ccccgacgct
     1861 cgaggatcag gaccggctca tcgtcaacaa ggccgtctac cggctgacgt cgccgcgccg
     1921 cggcgacatc gtgatgctgt actacccggt gaatcccgac aagtcgttcg tgaagcgggt
     1981 gatcgcggag gagggtgaca cggtgcggat caccgacgga cgggtgttcg tcaacgacct
     2041 cccggtggcc gacgactacg tgccgcccga gtaccggagc cacgacgact gggggccgca
     2101 ggtcatcccc gacggctact acttcgtgat gggcgatcac cgcaacaaca gttccgacag
     2161 ccggcactgg ggcatggtgc ccaagcggta catcatcggg cgggtgcagg cccgctggtg
     2221 gccgattccc gaggcgcggc tgttctgacg cgttccgggg ctacgcgcga gccggggacg
     2281 gctggtgcgc gtacagccgc tcgacgacgt ccgcccattc ctgctccacg accttccgcc
     2341 ggaccttcat cgtcggcgtc agctcgccgc cttcaacgga cagctccctg gggaggaggg
     2401 cgaagcgctt gatcttctcg aactgggcga gatcgacgtt gaccgcgtcc agcagatccg
     2461 cgtagagcgc cagcacgtcc ggcctctcga ccagctgcgc cttcgtccct gaagccgcgc
     2521 cctgcgcgcg cagacgctct tccagcagcg cgaagttcgg gacgatcagc gccgccggat
     2581 agctccgcgc ttcgccgacc aggacggcct cggccacgag cgggctgctc gtcaggcgct
     2641 gctcgatggg cgccggggcg atgttcttgc cgccggacgt cacgatgatg tccttcttgc
     2701 gatccgtgat cgtcaggtag ccgtcggcat ccagccggcc gatgtcgccg gtgtggagcc
     2761 agccgcccct gatggcatcc gcggtctccc ccggccggtt gtagtacccc tgcatgatgt
     2821 tcggcccgcg gacgaggatc tcgccgtcgt ccgcgatccg gacgtcgacg tccgggatgg
     2881 cgacgccgac ggtgcccggg cgcggggcgt ggcggggatt cacggtgacg cccggagagc
     2941 attcggtgag gccgtagcag ttgtagatcg gcacgccgac ggcgaacagg aactcctcgg
     3001 tcgtcctggc gagctgcgcg ctcccggaca cggcggtgcg caagcgtccg ccgagccgcc
     3061 ggcggatctt ccgcagcacc agcgcatccg ccgcgggccg ctggatgacg gcccggagcg
     3121 gcgcggggcg gccctcgaag cgggacctca cgatcgccgt cgacacgcgc atcgcccacc
     3181 gaaacagggc gcgattgagc ggcggccccg ccgcgacggc gtcgagcacg gcgtggtgga
     3241 acttctcgaa cacccgcggg acgccggtca tcaccgtcgg cctgacgcgc tgcaggtcgc
     3301 gcgcgatggt ctgcaggttc tccgcgaagg tcaccgtcgc gccgaccagc aggaacaggt
     3361 acagcaccac ccgctcgaag acgtggctga gcggcaggaa ggacagcggc acgtcgcgct
     3421 cgtcgatccc gaaggcggtg ttggccgccc acaggttcga gagcatgttg gcgtgcgtca
     3481 gcacgacgcc cttcggcggc ccggtcgtcc cggaggtgta gatgatcgtc gcgatatcac
     3541 ccggctcgac cgaccgggcc cgggcctcgt gctgcgcggc gaggccggga tcggcggcca
     3601 ggagggcctc gccccgcctg acggcgtcgg gcatggcgtg gacggcgggc cgtcccggac
     3661 cggcatcggg aagcggctgc gacggcgcga tcacgatgat cgccgccagc gcgggcaggc
     3721 ttgccgacac cccgaggagc ttggccagtt gcacgtcgtc ggacaccacg acggccttca
     3781 cgcctgcgtc ggccaggatg tactgtgttt gcgacgcggc gagcgtcggg tagacgggga
     3841 cgttgacggc gccagaggcg aggatcgcga ggtcggccat ggaccactcg ggccggctct
     3901 cgcagatcaa ccccacgcga tccccgcgcc cgatgcccat ctcctgcagg ctgaggctca
     3961 gcgccctcgt ctgcgacacg aaggcccgcg tggagaactc gtggaagccg tcggcgaggc
     4021 agcgccgcac gagcaccggc cggtcgtagc gctcggccag atagaacggc agttcggcga
     4081 acgtcctgac cgccgcgtgt ccgggcttcg gctggagcat ggctgggagt ctctacgcta
     4141 ggcgactccg cgctcgaggt caacggaaaa gaccctctgc ctgccaggcc gaccgcccgg
     4201 agagccggct cacctagtac cccgccgcct tgtcgacagt gttcctcagg cgttcgttct
     4261 ttgcgaaccg gccgaggttc tcggcgaaca ggtcgacggc cgcctcccag tagccggacc
     4321 ggaacccgcc aacgtgcgga gtgacgagca cgttcggcag gctccagagc gggctcgacg
     4381 cgtccagcgg ctcgcgatcg aagacgtcga gcgccgcccc ggcaatgcct ccacgcctca
     4441 gcgcgtcgac gagcgcggtt tcgttcacca gcttgccccg ggccacgttg atgagccagg
     4501 cggagggcct catcaggcga agctgggggg cgtcgatcat gccgcgcgtc tcgtcggtca
     4561 gcggcgcggc gagcaccacg aagtcggcgc acgccagcac ggtgggaagg ccctcggggc
     4621 cgaacgcgtc cgtgacgccc tccggcggcg ccccggccgc ggtcttgcgc gtcccgacga
     4681 ccgtcatgcc gagggcggcg gccgcgcgcg ccagcgcgcg cccgatcgct cccagtccga
     4741 caatcgccag cgtccggcct cggagcgtcg gcaggccggc gaggtcgtcg ccgatccact
     4801 tccgctcggc ctgggccagc acggcctcgg ggaatcgtcg cgccgaggcc agcatcagca
     4861 ggagggcgtg ctctgccacg gccgcggcgc tcatgccgcg cgagttcgtc aggacgaccc
     4921 ggctcgcccg catcgcgggg aacagcatcc cgccgactcc ggcggccgga ctgtgcaccc
     4981 agcggagctt cggggcggcg gcgagggccg ccggcgtcag ccgcgacgtg aaggcggcct
     5041 cggccgtccg gatgaggtcg atcatcgtgg cttcgtcgcc ggcctgcagg aaggcgtgct
     5101 ccgggaaccg ccggcgcagg gactcgaccg cctcggccgg gatgttccag gcgggttgct
     5161 cgctgaagat cgcgacgagc acgtccacgc tcacgccctc cgcgtccagg cgcccgatcg
     5221 ccctccccgc ttctccatca gccggatctc gccgatgacg gcgccgcggt cgaccgcctt
     5281 gaccatgtcg tagatcgtca gggccgccac ggcgacggcg gtcagggcct ccatctcgac
     5341 gccggtcggc ccggtcgtcc gggtccgcgc ctcgatctcg cagccgcggc gcccgagctt
     5401 cagcgtgacg tcgcagtgcg tcagggcgat cgggtggcac aacgggatga ggtcagctgt
     5461 cttcttcgcg gccaggatgc cggcgaggcg tgcggcttcg agcggatgac ccttggcgct
     5521 gcgtccgctc ctcacgagcc ggagcgtcga gggcgcaagc ctgacgaagc ctcgcgccac
     5581 cgcttcgcga accgtggccg gcttggcgct cacgtccacc atccgcgcgc ggcccttcgc
     5641 gtcggtgtgt gtgagccggg cgcccgtggg cggctcctgc gcgtcgattc cgcgcggcat
     5701 gtcacctcct ggcggcggcg cgcccgagca ggagccgccg gacttcggcc agctcgcccg
     5761 cggtcaccac gcggcagctg accaacagga tcgcgtacgc gatgagcgcg acggcgagca
     5821 ggaccgcgtt cagcggccag gcacctcgcg cgagcgccgg cggcacgaac accaccgcca
     5881 gggcgacggc ccgccccgcc gcggccagcg agtatcgcac gctgcgtctg gcgtgccgcg
     5941 tcaggaaggc ggcgtacagc gcggccgccg ccaccgcggc catcgccgcg ccgacgctgc
     6001 ccgcgcgcgc ggcgagcggc agtccgatcc cccagaatgc cgccagttcc agcgccgcgg
     6061 ccgccgtgac gatgcgcggc gggtcgacgg ccagcgccag caggcggccc acgtctcccg
     6121 ccgcgtggaa cagcatcgcc accatcaacg gcacgaggct cgccgcgacc ggcgcgtacg
     6181 accggcccag cacccacggc acggcgaccg ggccggcgac gacagcgccc gcgacgcaga
     6241 tcgcggcggc gatggccccg taggtgagca gccgctccag ccacgccgac acctcggcgc
     6301 ggcggccttg tccgagcagc cccacgatga acggcaggca ggcgaacgtg gcgtgccaga
     6361 gcacgaacgc cacggtcagg tagatcgaat acgccgcgcc caggtagccg acttcctcgt
     6421 agcggccggt ggccagccgc acgatgacct cgccgctgcg ctgcgtgatc gccgccagga
     6481 cggcggccac gccgaagccg ctgcccgccc gcaggaacgg agacaggtag cggcggctca
     6541 ggtcgagggc gtcccgcccg atgtacgccc ggacccacca gagaccgagg cccagcacca
     6601 cggcctcggc ggcggcgacg cccgcgcacg cgccccgcag gcctcccgcg acgaatcctg
     6661 ccggtgcgag cgcgagggtg agccagcggc gcagcagttc gcccctcccc caccgcccgg
     6721 cgtcgttgag gcccaggaac agggagaacg cgacgttgtt gatcgtccgg cccacgacgg
     6781 ccagggccac gagggcgctc gcgacgcgat ccggctcccg caggacgaat gccagaacgg
     6841 cgaagcagcc cgccgccgcc aacggcccgg tcagcgcccg cagcgccatg aggctcgtcg
     6901 tgaggcgccg gacggcatgg gtctggccct cggcgagcag gcgcgggacg gcgcgcgaca
     6961 tgaccgacac ggcgccgaag ccgctgagca gcgcgaacca cagcgacacc gacaggagga
     7021 gggcgaagcg accgaaccgg tccggcccca tcaggcgcgg gacgaaggcc gcaaacagcg
     7081 cggcggccag gatgtgcagg ccgcgctggg cgacgacttc tccgacgttg cgcagggtcc
     7141 ggtcccggtc gctggcggcc ccggggccgg gtgcggcggg ggcggtcgcg gctcccatct
     7201 ccatcaacgc gccgcattct atcccgaagg agcggggccc gcgccgggcg gcctgccgtg
     7261 gcgcggccgc catggcgcgg accgcgggcg gcgccgggct cgtgctaaga ttcgccgcta
     7321 tgagtcaccc agcttcctcc accccgccgt caggcggcca cgtgcccttc gtgcccgacg
     7381 ccgccgaaca gcgcgagttc acgctgcggg cgctcgtcct gggcctggtg atgtgcgtca
     7441 tcctcggcgc ggccaacgcg tacctgggcc ttcgcgcggg gatgaccatc gcggcaacct
     7501 acccggccgc cgtcatcggc atggcggtcc tcaggctgtt caggaacagc acgatcctcg
     7561 aggaaaactt cgccaggacc gtcgggtcca tcggcgagtc agtggcggcc ggcgccatct
     7621 tcaccatccc ggcgttcgtg attctcagcc tctggaagtt cgacgccgcg cacctgtggc
     7681 aggagtacct ggtctcgtcg gcgctcatga tcctcggcgg cctgctgggg atcatgttcg
     7741 tcacgattct tcgccgcgtg atggtggagg atccgtcgct gcagttcccc gagtcggtcg
     7801 ccgccagcga gatccacaag gccggccaga aagggagcga ggcggccctc cagctcttcc
     7861 gcgccatggg cgtcggcgcg ctcgtccgca tcgtgtcgag cgcgggattc gggctgttca
     7921 gggaccagaa cgaattcacg ctcccggtcg ggcggttgcg cgagagcttc gtgcggctgg
     7981 gcctgacgga cgagtccgcc aagatcaccg ccggcggcgt gacgaccttc tccgccccgg
     8041 acgtgacgcc ggcctacctg ggcgtcggct acatcatcgg cccggagctg ggcgcgctca
     8101 acttcgcggg cggcctcctg gcctggggcc tgttcgtccc gctcctggtc ttcttcctcg
     8161 ggcccggcct gatcgatcag tacctggcgg cgagcggcgg caacgaggaa gccgcgtggg
     8221 gggcgatggt cgtcgcactc tggaagtaca tcgtccggcc gatcgcggtg ggcggcatgc
     8281 tcgtcggcgc caccttcacg ctctacaaga tgcgcaagaa cctcatcggc ggcttcaagc
     8341 gaggcgtcgc cgacgtgaag aaatccgcga cggcgcaggc ggcgacgaag cggaccgagc
     8401 gcgatctgtc gctgaaggtg gtgggcctcg gcatcctggc catcttcgtg ctgatgatcg
     8461 ccctgtactc ctacttcacg ggcgcgatcg gcacggccgt gaccgccgcc gtcgtgatga
     8521 tcgtcacggg cttcttcttc gcggcggtgt cgggcaacct ggtcggcctg atcggctcct
     8581 ccaacaaccc cttgtcgggg ctcacgctcg cgacgacgat catcgcggcg ctgacgatgg
     8641 tgatcatcgg ggcgaccggc gcgcagggcg tcgtggcggt gctcggcgtc gcggccgtcg
     8701 gttgcgtgtc tgccgccgtc gccggcgaga tgctgcagga cctcaaggtg ggccacatcc
     8761 tcggcggcac gccgtggaag atgcagattg gcgacatctt cggcgtcgtc gtggccggcc
     8821 tggtgatgtt cttcccgctc tacatgatgc acaactccga cctcgcccag ttcggcgcct
     8881 ccggcggctt cggcggcagg acctacagcg ccccgcaggc gggcctgatg gcggcgctgt
     8941 cgcagggcat cgtcggcggc gaaatggcgt ggccgctggt gttcgtcggc atcgccatgg
     9001 gcatctcgct catcctgatc agggtccgaa gccccatgct cttctccgtc gggatgtacc
     9061 tgccgctcgg cacgaccttc gccatcttct gcggcggcgt catccgcggc gtcgtggaca
     9121 agatccgcga ccatcgcggg tacaacgcgg cgcagaaggc cagagtcgag aacgccggcg
     9181 tgctggcggc ctcgggcctg atcgccggcg aagccctcgt cggtctgctc atcgccggcg
     9241 tcgtctatgc gagggcatcg cacgcgttct ggacgtggcg ggacctcttc cagagccgcg
     9301 cgctggagtc actcgtgccc tggatgtcga tcgtcgccgc ggccgtgctg gtgtggtacc
     9361 tgatcgcggt gccgctcagg aaagcgggcg acgccgatga acccgccccg ccgacggcgg
     9421 tgatgtagca cggctggggg gcaggggcca ggcgctcgtg ggacgcggct gttgccgcgt
     9481 gggaggcgcg tcacgctgac cgcgcacgcc gacgagcgcc cggcgcccgg tctcctgccc
     9541 cttgtccctt gcctcttgtc cctcgtcagg gaaccaccct gaacgccgcc agcgtgacgg
     9601 cctgttcgcc gcccttcttc gccgacaggc ggacgatgta gtcgcctggc gccagaggcg
     9661 cgagcgccag ctccgcgcgg atccactgca cgaagtcgcg gctcatcgtc gacgtctcga
     9721 cgggcagcgt catcggcgcg ccgcgccgat cgaggagctc cgcgcttagc tcgtcgggcg
     9781 ccgacgacgc cgatgcgagg acgatgaggc gctctgtgcg acggagccgg cggtctggga
     9841 cggatgattc cgccgcggac actccggcgc cgcgccggac gcagaccggc tgaccgagcg
     9901 cgaagcgtcc ggcccccggc gcctccggga cggtgagccg caccgtgtcg gtggtcggca
     9961 ggccgccgcc cgccggctgc gcccgtacgc ggatcgtgta ggcgccggga tcgagccaga
    10021 cgtcctcggc ggacctcggg aaccggctct cgaagcgagc cgactcggcc gggattggca
    10081 cggtgtagga ggcgagcacc gcgcccccgc cggccgccac cgtgacctcg gcttcgccgc
    10141 cgcgggtcca cgcctcggcg cctcgcgcct tcgggtcgat cgcgccgaca atccagactg
    10201 ccggttcggc gccccggggc tggcccttca gcggttctcc ggccggcatc caccagcccc
    10261 aactcgcgga aagcgtgaag gctcggtccg gacggatcgc gtccagcgat gccagggctg
    10321 atctcagcgc gaccgtctcg gcgctcggcg ccggagccgc cgccgtcatc gtcgcggctt
    10381 ccgcctcggt cagggccagg tagccccgcc tcgcgcgcac ctcgattccg ggacgagcaa
    10441 cgcggacgcg gatcgcccgg tacttcccgt cgaacttcga gttcgtggag gtgtagccaa
    10501 gcaggtagta cgacgacagg tccgacgcca cccgcttcag cccgctgtcg atgtcgttcg
    10561 aatccatcac ggcgatgccg tccgtgttga gcgccgccgt gcgcagcgcc tcgtggcgcg
    10621 tccgcaccat cgccacgtca gtcgccggaa gagggggcgg gttcggtccg atgtcgttgt
    10681 cgaagacggc gaggcctctc ggctcgatag gatagaagct cgcgttcgcg cggttcgcca
    10741 ggttgaggat gttcaggtac cgctggcggt tgtcgatcat cgacagcgcc acgcggtcct
    10801 gatcgcactg ggcggtgttc gcgcccgcca tgttccgcgc gtccgtgttc ctgagcttgc
    10861 cgtcgggccc cacgaagacg cccggcatcc gcggcgtgcc gctctcggcc agcggcctgg
    10921 ccggccggta cagcatccac ccctcgctca cgaggaggat ggcttttcgc tcttcgcgca
    10981 ggccgccaag gtaacggacc agctcctcga tggcgtcgag ggtgaccttc tcgcgccggc
    11041 gctggatcat ggcctcggcc acctccgacg tcgcgcggcc cgtgcccggc gccggcgggt
    11101 agcactgctc gtacagctga tccaccgggt ctttcttcat ccgctcgtcc cgctggcccc
    11161 acatgcccgc gctgtcgagc atctccgcca ccaggtcggt gcggcgcgtg aaggtgatgt
    11221 cgctcgccgc catgatcggc gtcatgaccg cgagcaggtc ctccggcccg acgacg
//
LOCUS       DAOSIF010000038        11057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5838_length_11057_cov_3.688148, whole genome shotgun sequence.
ACCESSION   DAOSIF010000038 DAOSIF010000000
VERSION     DAOSIF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_11057_cov_3.688148"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLT35_02535"
     CDS             complement(<1..189)
                     /locus_tag="PLT35_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28133.1"
                     /translation="MKAGTSRAVVAVTAIPSGWTRSARACDADPPLAGGDLVVLQTDT
                     GAVLAEVSERVPVVSLFQP"
     gene            complement(226..1266)
                     /gene="holB"
                     /locus_tag="PLT35_02540"
     CDS             complement(226..1266)
                     /gene="holB"
                     /locus_tag="PLT35_02540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOG28134.1"
                     /translation="MTFRDIIGHRRLVRLLMRAAHAESLPPSLVFAGPEGVGKRQAAL
                     ALAQALNCLSPVTPADAARDGCGECAACRKISRGLHPDVIVVEPEGGLIAVDRARDAA
                     ERTAYRPFEGRTRVVVLDDADLMNDAAQNALLKTLEEPPARNVFVLVTARPNILLPTV
                     RSRCCLLRFAPLSASDVAAALAGRHGMSERAAQAAAALGGGSLGRALRFGAAGGTAAR
                     AAAVAALNEAAAAPSIRARLEVGASLLKASAKKPSTVDRGGKGQSDRASLAACLRAME
                     TLLRDLAVLTTRAPEAALVNLDLRAELEPLARRYDRGRLGHAFSAVGRSLLALERNAS
                     PKIVVDWLALQL"
     gene            complement(1396..1920)
                     /locus_tag="PLT35_02545"
     CDS             complement(1396..1920)
                     /locus_tag="PLT35_02545"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOG28135.1"
                     /translation="MADLTAQLARLRVPLGFVTGAAVLLVARPTWPLMAAGTAVACAG
                     EALRVWASGHLEKSREVTKSGPYRWFRHPLYVGSSILGLGVCIAAGSALIAALVLAYL
                     GATLPAAVVTEEGFLRREFGAEYDDYCRGVTAVQPRRFSWERALRNREYRAVAGLAIA
                     VTILALKAWLLPRP"
     gene            complement(1913..2932)
                     /locus_tag="PLT35_02550"
     CDS             complement(1913..2932)
                     /locus_tag="PLT35_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG28136.1"
                     /translation="MPQSVLIVRLGALGDLVHALPAAAAMRAAWPEATIDWLVDARYA
                     ALLALVPAVDRRIVVGRPGGATVAGAIKELRRAKYDLAVDLQGLLKSAVFARLSGARR
                     TAGFAAPHLRERAAGLFYTTRVGADDSGHVVWKNLSVAAALGARAERVEFPIDVPASP
                     APDAARRLLGLADGQPFAILNPAAGWPNKQWPAERYGAIAVHLARRHGLASLVLWGPR
                     ERALAEAVAAASSGAARVAPATDIGGVLALARNAAIFVAGDTGPMQLAAAVGAPIVGV
                     FGPTSPVRNGPWSPADLCLSRFDECECHHKRSCRRATPCIEAISIEEVAGAVDRRLEG
                     GRPRG"
     gene            complement(2933..3403)
                     /locus_tag="PLT35_02555"
     CDS             complement(2933..3403)
                     /locus_tag="PLT35_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOG28137.1"
                     /translation="MAQIVTLDRLLELAAADRAAGKSIALANGVFDLLHVGHIRYLEG
                     ARAEADVLVVAVNDDESVRALKGAGRPIMNESDRAEIIAALRCVDYVVVFSGTTVGDL
                     LLRLKPDVHCKGTDYTVETVPERPIVRSYGGRTAIVGDPKDHSTRDLLSRLRAR"
     gene            complement(3407..3982)
                     /locus_tag="PLT35_02560"
     CDS             complement(3407..3982)
                     /locus_tag="PLT35_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG28138.1"
                     /translation="MKRPAVFLDRDGTINLDAGYIDRLERLELYPFAIDAIRLFKRAG
                     YLVVILTNQAGVAQGLYGEEFVETVARHLAERASAGGGGIDGHYFCPHSPEAVVPAYR
                     VDCDCRKPKPGLALRAAADLDIDLPRSVVIGDRWRDLAVARAIGARAVLVKTGYGATE
                     ALHPPADGTAADAVCEDLIGAAVWLLDHPLP"
     gene            complement(3984..5012)
                     /gene="waaF"
                     /locus_tag="PLT35_02565"
     CDS             complement(3984..5012)
                     /gene="waaF"
                     /locus_tag="PLT35_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOG28139.1"
                     /translation="MTVLVFAPNWLGDAVMALPAVADLRRFAGSGTLVVAARPGIADL
                     FAMAPGIDRVLAVPAARRSGRLAALGASIETAKQASADVAVLLPNSFHAALAARRAGI
                     AERWGYRRQWRGWLLTRAVARPRGRLHQVDAYRRLVAALGLDNGPREPKLEAPPGAIE
                     AAGTLLSAAGWAPGTPLLGLAPGAAYGGAKRWPPERFAETAAGLARSLGTAPVLVGRE
                     ADRPAARAVAAELDRIGGPGGAVRAIDLIGRTSLRQLAGVMARCTAFVSNDSGAMHLA
                     AALGVPVAAVFGPTDERATAPLGARVRVVTARAWCRPCMLRECPIDHRCMTRIPARDV
                     IGAVEEVV"
     gene            complement(5009..5953)
                     /locus_tag="PLT35_02570"
     CDS             complement(5009..5953)
                     /locus_tag="PLT35_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG28140.1"
                     /translation="MKPLRYAVEYALVLATMGLARILPWRAVRGLGSALGGAFYALDG
                     RHRRVAMTNVAAAFPRKSARERRTLVRRTFGHFGRLLFELLKFSGLSREQMLERIAFE
                     GVETIRAAHAKGRGVLVFTGHFGYWEINAIAHAAAFGPVGVLARPLDNARLHGLLEGM
                     RGRTGNYVIYRRGALRRVLKALAAGDAVAILIDQHIQAPHAVMVNFFDRPAATTLALG
                     LLALRTGAPVVPVFSFPLGPGRYRLVYEHAVSPPADDSPDAIRDFTQRCTDVFEMQVR
                     RYPELWLWMHRRWRDAGGPSSGPGMFPEAAPEKGGGEE"
     gene            complement(5950..7005)
                     /gene="lpxK"
                     /locus_tag="PLT35_02575"
     CDS             complement(5950..7005)
                     /gene="lpxK"
                     /locus_tag="PLT35_02575"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOG28141.1"
                     /translation="MLGALYAAATARRRAWLRDGRQRRLRQPVVSVGNLRVGGTGKTP
                     TAAYVAGLLADAGERPAVLSRGYARRTAPDGVVVVSDGERLLADLGRSGDEPLMLARA
                     VRAARVVVSPDRYLAGRLAETRLGATVHVLDDGFQHVGLARGTDLLILDREDAADPRT
                     LPAGRLREGLGAARFADALLVPGASEAEAASLAARLGVSTAFALVRSTGPHYRLGGGA
                     GAAAGAAAGRVYAFAGIARPARFFAELGAAGWDVAGTEAFPDHHRYAARDVAALAAAA
                     RRAGAGALVTTEKDAVRLLPLRPLPAPVLVVPLAVRVEPACAFAEWLAARLAAERRAA
                     SPAAAPPARPQAEGGGP"
     gene            complement(7016..8350)
                     /locus_tag="PLT35_02580"
     CDS             complement(7016..8350)
                     /locus_tag="PLT35_02580"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOG28142.1"
                     /translation="MYVLYSAAMLLLFAVATPYFLCQWARHGKYAVNLPERLGALPVA
                     LNLDREPSIWIQTVSVGEALTARPIAAGLKERYPHLRLYVSTTTITGQQVARESFPMA
                     DGVFYFPIDLPVIVRRVLGIVRPRLFVMMETEIWPNLLRECRARGVRTVLVNGRVSAR
                     SFPRYRLVRPLFRRVLASVDLFCVQGPETAQRLIALGAPPGRIAVTGSLKFDALHRPG
                     SAQPSSRAGDRVLRFFRVAAGRAIIVAGSTMPGEHEIVLAALERVRTLAGNPLLVLAP
                     RRPEHFGDAEARALERGFRTVRRSALTVDEEVKADVVILDTIGELAEVYQIATAVFVG
                     GSLVNIGGHNILEPAAFGKPVVFGPHMQNFAEIASAFLEGRAAEQVRSGRELEDALIR
                     LLADPAERERMGAAARAIVEANRGATARTLDAIAGILPPAGAERVLPFPGAR"
     gene            8485..9440
                     /gene="gap"
                     /locus_tag="PLT35_02585"
                     /pseudo
     CDS             8485..9440
                     /gene="gap"
                     /locus_tag="PLT35_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            9572..10765
                     /locus_tag="PLT35_02590"
     CDS             9572..10765
                     /locus_tag="PLT35_02590"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOG28143.1"
                     /translation="MAKLSIRDLDLGGKRVFIRVDFNVPIKEGRIKDDTRVRASLATI
                     KYALEQGATVILASHLGRPKGGPSPEFSLAPVAAHLGTLLGRPVTFASDCVGEPARQA
                     VAEARAQGNNVVVLENVRFHKEEEKNDPAFAAQLAELADLYVNDAFGSSHRAHASTEG
                     MAKLLPQAASGFLMEDELKYLGLALESPERPFVAVLGGAKVSDKIEVIENFLGRVDAL
                     LVGGAMAYTFFKAKGLPVGKSLVEDDRLDAARAVMAKAAEKGVRLELPVDHVVAEKIE
                     AGVATETLAVDDPAIGDRMGLDIGPKTAALYAGILATAKTVVWNGPMGVFETSPFDAG
                     TNAVAKAVAGVKGTTIIGGGDSVSAVSKAGVTSQITHITTGGGASLEFLGGQTLPGVA
                     ALTEK"
BASE COUNT         1609 a         3957 c         4133 g         1358 t
ORIGIN      
        1 tggctgaaag agcgacacga ccggcacgcg ctccgacacc tccgccagca ccgctcccgt
       61 gtcggtctgc agcaccacca ggtcgccgcc ggcgagcggc gggtccgcgt cgcacgcccg
      121 tgccgagcgg gtccagccgg agggaatcgc ggtcaccgcc acgacggcgc gggacgttcc
      181 agccttcatc ggccctgcat gtatggccgt cggggggctc cgccgctaga gctggagcgc
      241 gagccagtcc accacgatct tggggctcgc gttgcgctcc agcgccagga gcgaacgccc
      301 gacggcggaa aacgcgtgcc cgagccggcc gcggtcgtaa cgcctcgcca ggggttccag
      361 ctcggccctg agatcgaggt tgaccagcgc cgcctccggc gcgcgggttg tcaacacggc
      421 gaggtcgcgc agcagcgttt ccatcgcccg caggcaggcg gccagcgacg cgcggtcgga
      481 ctgccccttg ccgcctctgt ccactgtact cggctttttc gccgacgcct tcaggagact
      541 ggcgccgacc tccagccgcg cccggatgct cggcgccgcg gcggcttcgt tcagcgcggc
      601 gacggcggcg gcgcgcgcgg cggtgccgcc tgctgcgccg aacctgagcg cccgcccgag
      661 gctgccgccg ccgagcgcgg ctgccgcctg cgccgcgcgc tcgctcatgc cgtggcgccc
      721 cgccagggcc gccgccacgt ccgatgcgga gagcggcgcg aacctcagca ggcagcagcg
      781 cgaccgcacg gtcggcagca ggatgttcgg acgcgccgtc accagcacga acacattgcg
      841 cgcaggcggc tcctcgaggg tcttgagcag cgcgttctgc gcggcgtcgt tcatgaggtc
      901 cgcgtcgtcc aggaccacca cgcgcgtccg gccctcgaag ggccggtagg ccgtccgttc
      961 ggccgcgtcg cgcgcccgat ccacggcgat gagcccgcct tccggctcga cgacgatgac
     1021 gtcgggatgg agcccgcgcg agatcttcct gcacgcggcg cactcgccgc agccgtcgcg
     1081 cgcggcgtcc gccggagtga ccgggctcag gcagttcagc gcctgcgcca gcgccagcgc
     1141 cgcctgccgc ttccccacgc cctccgggcc cgcgaagacg aggctcggcg ggagcgactc
     1201 ggcgtgggcc gcgcgcatca gcagccgtac caggcgacga tggccgatga tatcccggaa
     1261 ggtcatggga ggcagagagt tacgggaatg ctaacacatt agggattggg gatcggagat
     1321 tgggcgtcgg gattcgggac tcgggactcg ccttgcacgc cggggacgag ggacaaagag
     1381 cagcaagcgg gagaactacg gccgagggag gagccaggcc ttgagggcca ggatcgtcac
     1441 ggcaatggcg aggccggcga cggcgcggta ctcccggttg cgcagggcgc gctcccaact
     1501 gaagcgcctg ggctggaccg ccgtgacgcc gcggcaataa tcgtcgtact cggctccgaa
     1561 ctcccgccgc aggaacccct cctcggtcac gacggcggcc ggcagcgtcg cgcccaggta
     1621 cgcgagcacg agcgcggcga tgagggcact gccggcggca atgcagacgc caaggcccag
     1681 gatcgacgac ccgacgtaca gcgggtggcg gaaccagcgg tacgggccgg acttcgtcac
     1741 ctcgcggctc ttctccaggt ggcccgacgc ccagacccgc agcgcttcgc ccgcgcaggc
     1801 cacggcagtc ccggcggcca tgagcggcca ggtgggccgc gcgacgagca gcacggccgc
     1861 gccggtgacg aagccgaggg ggacccgcag ccgggcgagt tgcgcggtga ggtcagccac
     1921 gcgggcgccc tccctccagc cgccggtcga cggcgccggc gacttcctcg atgctgatgg
     1981 cctcgatgca gggcgtggcg cgccggcacg atcgcttgtg gtggcactcg cactcgtcga
     2041 agcgggacag gcacaggtcc gcggggctcc acgggccgtt gcggacgggg ctcgtcggcc
     2101 cgaacacgcc gacgatgggc gcgccgacgg cagccgcgag ctgcatcggg cccgtgtcgc
     2161 cggcgacgaa gatcgcggcg tttcgagcca gcgccaggac gccgccgatg tcggtggcgg
     2221 gcgccacccg cgccgcgccc gaggaggcgg ccgccaccgc ttcggcgagc gcgcgctccc
     2281 tcgggcccca cagcacgagc gacgccaggc catgccggcg ggccaggtgg acggcgatgg
     2341 cgccgtagcg ttccgccggc cactgcttgt tcggccagcc ggcagccggg ttcaggatgg
     2401 cgaatggctg gccgtccgcg aggccgagca gccggcgcgc ggcgtcgggc gcaggcgacg
     2461 ccggcacgtc gatggggaac tccacgcgct cggcgcgcgc gccgagcgcc gccgcaaccg
     2521 acaggttctt ccagacaacg tggccgctgt cgtcggcgcc gacgcgcgtc gtgtagaaga
     2581 ggccggccgc gcgctcgcgc aggtgggggg cggcgaagcc cgccgtgcgg cgcgcccccg
     2641 acagccgggc gaagaccgcc gacttcagca ggccctgcag atccacggcg aggtcgtact
     2701 tcgcgcgccg cagctccttg atcgcgccgg ccacggtggc gcctccggga cgtcccacga
     2761 cgatccgccg gtcgacggcg ggcacgagcg ccagcagcgc cgcgtagcgc gcgtcgacga
     2821 gccagtcgat cgtcgcctcc ggccaggcgg cgcgcatcgc cgccgccgcc ggcagcgcgt
     2881 gcacgaggtc gccgagcgcg ccgagccgga cgatgaggac cgactgcggc atctagcgcg
     2941 cccggaggcg cgacagcagg tcgcgcgtcg aatggtcctt gggatcgccg acgatggccg
     3001 ttctcccgcc gtaggaccgg acgatggggc gttcgggcac ggtctcgacg gtgtagtccg
     3061 tgcccttgca gtgcacgtcg ggcttcaggc gcagcaggag atcgccgacg gtggtccccg
     3121 agaacacgac cacgtagtcc acgcaccgca gggccgcgat gatctcggcc cggtccgact
     3181 cgttcatgat cggccgcccg gcccccttca gcgcccgcac cgactcgtcg tcgttcaccg
     3241 cgacgaccag cacgtccgcc tcggccctgg cgccctcgag gtagcggatg tggccgacgt
     3301 gcagcaggtc gaacacgccg ttggccaaag cgatggactt cccggcggcg cgatcggcgg
     3361 ccgcgagttc gagcaggcgg tcgagggtga caatctgggc catggctcag ggcagcggat
     3421 ggtcgaggag ccagaccgcc gcgccgatga gatcctcgca caccgcgtcg gccgccgtcc
     3481 cgtcggcggg cggatggagc gcttcggtcg cgccgtagcc ggtcttgacg agcacggcgc
     3541 gcgcgccgat ggcgcgggcg acggcgagat cgcgccagcg gtcgccgatg acgaccgatc
     3601 gcggcaggtc gatgtcgagg tcggcggccg cgcgcagggc caggcccggc ttcggcttgc
     3661 ggcagtcgca gtccacgcgg tacgccggca cgacggcttc cggcgagtgg gggcagaagt
     3721 agtgcccgtc gatgccgccg ccgcccgccg acgcgcgctc ggccaggtgc cgcgcgaccg
     3781 tctcgacgaa ctcctcgccg tagagcccct gggcgacgcc ggcctggttg gtcaggatga
     3841 cgacgaggta gccggcccgc ttgaagaggc ggatggcgtc gatggcgaac gggtagagct
     3901 ccaggcgttc cagccggtcg atgtagcccg cgtccaggtt gattgtgccg tcccgatcca
     3961 ggaagaccgc cggtcgcttc acctcacacc acctcttcga cggcgccgat gacgtcgcgc
     4021 gccgggatgc gcgtcatgca ccggtgatcg atcgggcact cgcgcagcat gcagggccgg
     4081 caccacgcgc gcgcggtcac gacgcgcacg cgcgcgccga ggggggccgt cgcgcgctcg
     4141 tcggtcgggc cgaacacggc cgccacgggc acgccgagcg ccgcggcgag gtgcatcgcc
     4201 cccgagtcgt tggacacgaa ggcggtgcag cgcgccatca ctccggccag ctggcgcagg
     4261 ctggtgcgcc caatcaggtc gatggcgcgg accgcgccgc ccggcccgcc gattctatcg
     4321 agttccgccg cgacggcgcg agcggccggc cggtccgcct cgcggccgac gaggaccggc
     4381 gccgttccca ggctgcgcgc gaggccggcg gcagtctccg cgaaccgctc cggcggccag
     4441 cgcttcgcgc cgccgtaggc cgcgcccgga gccaggccga ggaggggcgt cccgggcgcc
     4501 cagccggcgg cagacagcag ggtgccggcc gcctcgatcg cgccgggcgg ggcctccagc
     4561 ttgggctccc gcgggccgtt gtccaggccc agggccgcca cgaggcggcg gtaggcgtcc
     4621 acctggtgca ggcggccgcg cgggcgcgcc accgcgcgcg tcagcagcca gccgcgccac
     4681 tggcgccggt agccccaccg ctcggcaatc ccggcgcgcc gggcggcaag cgccgcgtgg
     4741 aaggaattcg gcagcaggac cgccacgtcg gccgacgcct gcttcgctgt ctcgatgctg
     4801 gcgccgagcg cggccaggcg ccccgatctg cgggccgcgg gaaccgcgag gacgcggtcg
     4861 atgccggggg ccatggcgaa caggtccgcg atgcccgggc gcgccgcgac gacgagcgtc
     4921 cccgagccgg cgaagcgccg gagatcggcg acggcgggca gcgccatcac ggcgtcgccg
     4981 agccagttcg gcgcgaacac gagcaccgtc attcctcgcc tccgcctttc tccggtgcgg
     5041 cctcggggaa catgcccggc ccggaggagg gccccccggc gtcgcgccag cggcggtgca
     5101 tccacagcca cagctccggg tagcggcgca cctgcatctc gaacacgtcg gtgcagcgct
     5161 gcgtgaagtc gcggatggcg tccggcgagt cgtcggcggg cgggctgacg gcgtgctcgt
     5221 acaccaggcg atagcggccc ggcccgagcg gaaacgagaa gacggggacc accggggcgc
     5281 cggtccgcag ggccagcagg ccgagggcga gggtcgtggc ggccgggcgg tcgaagaagt
     5341 tcaccatcac cgcgtggggc gcctggatgt gctggtcgat caggatcgcg acggcgtcgc
     5401 cggccgccag cgccttcagc acgcgcctga gcgcgccgcg ccggtagatg acgtagttgc
     5461 cggtgcggcc gcgcatgccc tcgagcaggc cgtggaggcg ggcgttgtcg aggggccgcg
     5521 ccaggacgcc gacgggcccg aaggccgcgg cgtgggcgat ggcgttgatc tcccagtagc
     5581 cgaagtggcc cgtgaagacc agcacgccgc gccccttcgc gtgcgcggcg cggattgtct
     5641 cgacgccctc gaatgcgatc cgctccagca tctgctcccg ggagaggccg ctgaacttca
     5701 gcagttcgaa cagcaggcgg ccgaagtgcc cgaaggtccg gcgcaccaag gtgcgccgct
     5761 cgcgggcgga cttgcgcggg aaggcggcgg ccacgttcgt catcgcgacg cgccggtgcc
     5821 ggccgtcgag cgcgtagaag gcgccgccga gcgccgagcc cagcccgcgc acggcgcgcc
     5881 acggcaggat gcgcgccagg cccatcgtcg cgaggacgag ggcgtactcg acggcgtagc
     5941 gaaggggctt cacggcccgc cgccttccgc ctgcggccgc gccggcggcg cggcggcggg
     6001 gctggcggcg cggcgctcgg cggcgaggcg ggcggccagc cattcggcga aggcgcacgc
     6061 cggctccacg cgaaccgcga gcggcacgac gagcaccgga gcgggcagcg ggcggagcgg
     6121 cagcagccgc acggcgtctt tttcggtcgt gacgagcgcg cctgcgcccg cgcgccgcgc
     6181 cgccgcggcg agcgccgcca cgtcgcgcgc cgcgtagcgg tggtggtccg gaaacgcctc
     6241 ggtgcccgcg acgtcccagc cggcggcgcc gagttccgcg aagaagcggg cggggcgcgc
     6301 gattccggcg aacgcgtaga cccggcccgc cgccgcgccg gccgccgcgc ccgcgccgcc
     6361 gcccagccga tagtgcggcc ccgtgctgcg gacgagggcg aacgccgtcg agacgccgag
     6421 gcgcgcggcg agcgaggccg cctcggcttc gctcgcgcca ggcacgagca gcgcgtcggc
     6481 gaacctggcg gcgccgaggc cctcgcgcag cctccccgcc ggcagcgtgc gcgggtccgc
     6541 ggcgtcttcg cgatcgagga tgagcaggtc cgtgccgcgg gccagcccga cgtgctggaa
     6601 gccgtcatca agcacgtgca ccgtggcgcc gaggcgggtt tcggcgaggc ggccggcgag
     6661 gtaccggtcg ggtgacacca ccacccgggc ggccctgacg gcccgggcca gcatgagcgg
     6721 ctcgtcgccg ctgcgcccga gatcggcgag cagccgctcg ccgtccgaca ccaccacgac
     6781 gccgtccggc gccgtgcgcc tggcgtatcc ccggctgagc accgccggcc gttcgccggc
     6841 gtcggccagc agcccggcga cgtacgcggc cgtgggcgtc ttgcccgttc cgccgacgcg
     6901 caggttgccg acgctcacga ccggctggcg caggcggcgc tgcctgccgt cccgaagcca
     6961 ggcccggcgc ctggctgtcg ccgccgcgta gagcgcgccc agcatcggtc gatcctcagc
     7021 gggcgcccgg aaacggcagg acgcgttcgg cgcccgcagg cggcaggatc ccggcgatcg
     7081 cgtcgagcgt tcgcgcggtc gcgccccggt tcgcctccac gatcgcgcgc gctgccgctc
     7141 ccattcgctc gcgctcggcc ggatccgcca gcaggcggat gagcgcgtcc tccagctcgc
     7201 gccccgatcg cacctgctcg gccgcgcggc cctccaggaa cgccgaggcg atctcggcga
     7261 agttctgcat gtgcggcccg aagacgacgg gcttgccgaa ggcggcgggc tccaggatgt
     7321 tgtgcccgcc gatgttgacc aggctcccgc cgacgaacac cgccgtcgcg atctggtaga
     7381 cctcggcgag ctcgccaatc gtgtcgagga tcacgacgtc ggccttcacc tcctcatcga
     7441 ccgtcagcgc cgaccgcctc accgtcctga acccccgctc gagggcgcgg gcctcggcat
     7501 cgccgaagtg ctccggccgg cgcggcgcga ggacgagcag cgggttcccg gcgagcgtcc
     7561 ggacgcgctc gagggccgcc aggacgatct cgtgctcgcc cggcatcgtg ctgcccgcga
     7621 cgatgatcgc gcggccggcg gccacgcgga agaagcgcag cacgcggtcg ccggcccggg
     7681 aggacggctg ggctgagccc ggccggtgca gcgcgtcgaa cttcaggctg ccggtcacgg
     7741 caatccggcc gggcggggcg cccagggcga tcagccgctg cgccgtttcc gggccctgca
     7801 cgcagaagag gtccaccgac gccagcaccc gcctgaagag cgggcggacc aggcggtagc
     7861 gcgggaacga ccgcgccgag acccggccgt tgacgaggac cgtcctgacg ccgcgggcgc
     7921 ggcactcccg caggaggttg ggccagatct cggtttccat catcacgaag agtcgcggcc
     7981 ggacgatgcc gagcacgcgg cgcacgatga cgggaaggtc gatcgggaag tagaacacgc
     8041 cgtcggccat cgggaagctc tcgcgggcga cctgctgccc cgtgatggtc gtggtggaca
     8101 cgtagagccg gaggtgcggg taccgctcct tgagccccgc ggcgatcggc cgggccgtca
     8161 gcgcctcccc caccgacacg gtctggatcc agatcgacgg ctcccggtcc aggttcagcg
     8221 cgaccgggag ggcgcccagc cgctcgggga ggttgacggc gtacttcccg tgcctggccc
     8281 attggcacag gaagtagggc gtcgcgacgg caaagagcag gagcatcgcg gcgctgtaga
     8341 ggacgtacat ggccggctcc aagataccac gcgggtttga ccccccagca agccgttcgg
     8401 tacaattggg cgtttgttcg cgaaggagaa gccatggcaa tcaaggtcgg catcaacgga
     8461 ttcgggcgga tcggccgcaa catcatgcgc gccgccatcg gcaacaagga catcgacttc
     8521 gtggcggtga cgacatcacc gacaccaaga cgctggcgca cctgctcaag tacgactcca
     8581 tcctgggcaa cctgacggcc acgatcgagg ccggcgacgg ctggatcagc gtgaacgggg
     8641 acaggttcca ggtgctgtcg cagaaagacc cgtcgctgct cccgtggaag gacctcggcg
     8701 tcgaggtggt gttcgagggc acgggcaagt tcaccaagcg cgacgacgcg atgaagcacg
     8761 tcgcggccgg agccaggcgc gtcgtcatca ccgcgccggc cacttcgccg gacgtgacgc
     8821 tgctgatggg cgtgaaccac gaactgtacg acccggcgaa gcacgtgatc atctcgaacg
     8881 cgtcgtgcac gacgaactgc ctggcgccgg tggcgaaggt cctgcaggag agcttcggca
     8941 tccgcaaggg ctggatgacg accatccaca gctacacgaa cgaccagaac gtgctggact
     9001 tcccgcacaa ggacctgcgc cgcgcccgcg cggcggcgct gtcgatgatc ccgacgacca
     9061 ccggcgcggc caaggcgatg ggcgaggtga tgccggccct caagggcaag ctggacgggt
     9121 tctccatgcg cgtgccgacg ccgaacgtgt cggtggtgga cctggtggcg ctgctcgaga
     9181 agccggcgac cggcgaggag atcaacgccg cgttccgcgc ggctgcggcc gggccgctga
     9241 agggcatcct ggcggtcgag gacgcgcccc tcgtgtcgat ggactaccgc ggcaactcga
     9301 actcgtccat cgtggacgcg gcgtacacga aggtcatgga cggcgacttc gcgaaggtcc
     9361 tcgcgtggta cgacaacgag tgggcctact cggtgcgctg catcgacctc gcgctgtaca
     9421 tggcgaagaa ggggctctag gtaggggcta ggcgctaggc gctagtaggg acaaggggca
     9481 aggggcaggg tgccgggcgc tggcagcccc cgagccccag cccctggtcc ccgatccccg
     9541 cagtcagcaa cgccggtcag ggagaactgc cgtggccaag ctgtcgattc gcgatctcga
     9601 tctcggcggg aagagggtct tcatccgcgt cgatttcaac gtgccgatca aggaagggcg
     9661 catcaaggac gacacgcgcg tccgggcgtc gctcgcgacg atcaagtacg cgctcgagca
     9721 gggcgcgacc gtgatcctcg cctcgcacct cgggaggccg aagggcgggc cgagccccga
     9781 gttctccctc gcgccggtgg cggcccacct cgggacgctg ctgggccggc cggtgacgtt
     9841 cgcctccgac tgcgtgggcg agcccgcgag gcaggccgtc gccgaggcgc gggcccaggg
     9901 caacaacgtg gtcgtgctcg agaacgtgcg cttccacaag gaagaggaga agaacgaccc
     9961 ggcgttcgcg gcgcagctgg ccgagctggc cgacctgtac gtgaacgacg cgttcgggtc
    10021 cagccaccgc gcccacgcgt ccaccgaggg catggcgaag ctcctgccgc aggcggcttc
    10081 gggcttcctg atggaggacg agctgaagta cctcggcctc gcgctggaat cgcccgagcg
    10141 gccgttcgtg gcggtgctcg gcggcgcgaa ggtgtcggac aagatcgagg tcatcgagaa
    10201 cttcctgggc cgcgtggacg ccctgctcgt cggcggcgcg atggcctaca ccttcttcaa
    10261 ggcgaagggg ctgcccgtcg gcaagtccct cgtcgaggac gatcgcctcg acgcggcgcg
    10321 cgccgtcatg gccaaggcgg cggagaaggg cgtccggctc gagctcccgg tggatcacgt
    10381 cgtggccgag aagatcgagg cgggcgtggc caccgagacg ctggcggtgg acgacccggc
    10441 gattggagat cggatgggcc tcgacatcgg cccgaagacc gccgccctgt atgcggggat
    10501 cctcgcgacg gcgaagaccg tggtgtggaa cggcccgatg ggcgtgttcg agaccagccc
    10561 cttcgacgcc ggcacgaacg ccgtggcgaa ggccgtggcg ggcgtgaagg gcacgacgat
    10621 catcggcggc ggcgactcgg tgtcggcggt gtcgaaggcc ggcgtgacgt cgcagatcac
    10681 gcacatcacg accggcggcg gcgcgtcgct cgagttcctg ggcggccaga cgctgcccgg
    10741 ggtcgcggcg ctgaccgaga agtagggctg cggcgcccgg tcctgagcga gctcctgacg
    10801 tcgcggagcg agccgatggg cccggcgcag cccaacagga tggagcccgg gggcttcagc
    10861 ccccgggtcg tgagccctct tgttccttgc ctcttgtgcc ttgtctcttg tgccttggcc
    10921 cctggtgagt agccgcccat gcgcattccc gtactggccg ccaactggaa gatgttcaag
    10981 accgtcggcg agacggtctc gtacttgaag gagttctgcg ccctcaccgc cggcgtgagc
    11041 ggggccgaga tcgcggt
//
LOCUS       DAOSIF010000039        10915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5922_length_10915_cov_4.199908, whole genome shotgun sequence.
ACCESSION   DAOSIF010000039 DAOSIF010000000
VERSION     DAOSIF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5922_length_10915_cov_4.199908"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLT35_02595"
     CDS             complement(<1..307)
                     /locus_tag="PLT35_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28144.1"
                     /translation="MDFSELANGLAASPVVFCQTTEFFKQLIVRDLLVSVRCLLFFAA
                     HDDSVFAPGRGEALEYRRETAPDDLFVKLGEFPADSDPAVADEAEKVLERSADAVRRF
                     "
     gene            449..1204
                     /locus_tag="PLT35_02600"
     CDS             449..1204
                     /locus_tag="PLT35_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28145.1"
                     /translation="MAKIRRGSRRQASVKKAKRAVPAKTKRSASSAKRPSRPAARPST
                     RSSARPRTAPPAKPSGRPSAKPAPQATPARRSTYADAIILYERGLHALQAKRYREASA
                     TLRRVIAEFPEEIELHERAELYIRVCERQMAPPDATPKTPDEQVLAATLAMNAGAFDQ
                     AAALLTSVLQAQREHDGAEYLLGVALAAKGDASGALLHLARAIDLNPENRDLLRKAPE
                     LEALRQTDGGRALLAEASSAARRERRNQPRVRH"
     gene            1229..1981
                     /locus_tag="PLT35_02605"
     CDS             1229..1981
                     /locus_tag="PLT35_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOG28146.1"
                     /translation="MVHQSVIDALLAAGVTLLDPRTTFIDEGVTIGAGTIVHPGVTLE
                     GRTRVGARCRLRSWVRIADSDLGDDVIVNDSCVILESRVDDGCSVGPFAHLRPGSHMK
                     RGAHVGNYVELKKTVLGEGSKASHLTYLGDAAIGANVNIGAGTITCNYDGARKHPTTI
                     GDGAFVGSGSQLVAPVAVGESAYVAAGSTITEDVPPGALGIARGRQRNVEGWAARRRR
                     QTEAPVPAHDSSASASQPSLRGPEGGSPAEGS"
     gene            2016..4364
                     /locus_tag="PLT35_02610"
     CDS             2016..4364
                     /locus_tag="PLT35_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG28147.1"
                     /translation="MDLLSSLNPPQREAVVYTDGPLLILAGAGSGKTRVIAHRAAYLA
                     GSGLAAPESILAVTFTNKAAEEMRDRVGALLGGSAGGMWISTFHAFCARLLRREAIRA
                     GLSRDFVIYDSSDQLAAVKQVLRDLGVEDTLLAPRAALARISHAKNRMESPASMPGRH
                     ASRRDEAAARVYAEYVKVLAANGALDFDDLLLRAVDLLDRAETVREAYSRRFRFVMVD
                     EYQDTNRPQYLLMRRLAEHHRNLTVVGDPDQSIYAWRGADIRNILDFETDFPEAKVIR
                     LEQNYRSTQVILDAASAVIARNVNRKEKRLWTDRSGGDLIVCARCGDEIEEADFAART
                     ARRVLAADPRASAALLYRTNAQSRALEDALRREDLPYRIVGSVRFYERKEIKDALAYL
                     KLLLNPADDLSLRRIINTPARGIGKGVLDALEAHRAAGPAGLAFGAPAGLGPPAGGAQ
                     APIAAPAPDPLRWSLWAALEHGLEARRFPARAAASLTAFRDLIRSLANMARRGPASVA
                     LATVLDRTGYLQDLRDERSEEAQGRIENLAELVSAAREFESRDPEPSLAGFVDRLSLL
                     SEADESEGARDARVLLMTLHAAKGLEFPAVIVTGLEEGLFPHARSADEDGSLEEERRL
                     CYVGMTRARQQLFLTSAARRRVFGEYRPTEPSRFLDEIPSSLVQRYEFVGAGASPTAP
                     DAADFAFRFSRRPGRQGAAGFRSSTDASGAPRGLTYSPEDEDQSAHRSGAGLGMRVRH
                     PRFGLGTVLAIEDHADDVKLTVRFDTAGVKRLVGKYARLERA"
     gene            complement(4427..5113)
                     /locus_tag="PLT35_02615"
     CDS             complement(4427..5113)
                     /locus_tag="PLT35_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28148.1"
                     /translation="MVPALVLAVVASACGGGEEQILLNKYFMASKVSDNMTLSNIATV
                     AFDPKVDGQMQSFKVLSISEPAVAPLTIKARAQELKAASDEEKAFSEKKKEYQDANAD
                     GIDRVLKAEAKNQPLRGKDAEIQKEWTKWREETAAHAKKVSEIRKQVAAEQPIVEISC
                     QDQRNPIDTTAYEGESTTKDVTIEGKVKTDAGVATKKYVFTLQRITLQGVNGRELVGR
                     WVVAGRKDAE"
     gene            complement(5155..7617)
                     /gene="gyrA"
                     /locus_tag="PLT35_02620"
     CDS             complement(5155..7617)
                     /gene="gyrA"
                     /locus_tag="PLT35_02620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG28149.1"
                     /translation="MDTASRIPVAIEDEMKRSYLDYAMSVIIGRALPDVRDGLKPSHR
                     RVLYGMRLMGLGSGRAHRKSAKIVGEVMGNFHPHGDSAIYNTLVRMAQDFNMRYPLVD
                     GQGNFGSVDGDPPAAMRYTEARLKALAEELMADLDKDTVDFAPNYDETTEEPGVLPAP
                     FPNLLVNGSAGIAVGMATNIPPHNLGEAVDCVIYAIEHPEAGADQKLSAMWRLMPGPD
                     FPTGGIIVGRAGIAQAYRTGRGSILVRARTEIETAKKGDRTSIVVREIPYQVNKARLI
                     EKIADLVRDKTIEGIADLRDESDRDGMRIVIDLKRGEVPDVVLNNLFKHTALQVSFGV
                     IMLAIVGGRPRVLPLVEMVEQFADFRRDVVRRRIRFELRKAEARAHILEGLKIALDHL
                     DAVIALIRASRNPAEAREGLMASFGLSAIQAQAILDMQLQRLTGLERQKIEDELAEVL
                     QTIERLRAILADERLLMATVIQELRDVRQAFADARRTEIIDESGEFRIEDLIADEDMA
                     ITVTHTGYVKRTAVTSYRNQQRGGKGRIGMRFREEDFVDHLLIASTHDSLLVFSDRGR
                     AYWLKVHEIPDVGPDGKGKAIANLVSMAAGEKIASLLPGRAWEEDKFVFMGTRKGVVK
                     KTSLTAFQNPRAGGIIAMGVAEDDAVIAVQVTDGTREIFLASHDGMAIRFPETDVRPM
                     GRTAYGVRGISLREGDSVVAMEVVEPGGGGTVLTVCENGYGKRTALEEYRVQSRGGVG
                     IISIHTSDRNGRVAGAVYVRDEDELMLITQLGKVLRMPTSGIRAIGRVTQGVRLIEIE
                     AEDRVVSVARLAEREDETATGV"
     gene            complement(7688..10222)
                     /gene="gyrB"
                     /locus_tag="PLT35_02625"
     CDS             complement(7688..10222)
                     /gene="gyrB"
                     /locus_tag="PLT35_02625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOG28150.1"
                     /translation="MAAADDRMRLEEAPGVSAEENLVGPGGTDAGDYSADKIKVLEGL
                     EAVRKRPAMYVGSTGPVGLHHLVYEVVDNSIDEALAGYCDEIRVTIHTDNSITVVDNG
                     RGIPVDMHESGRPAAEVVMTVLHAGGKFDNASYKVAGGLHGVGVSVVNALSETLDLEI
                     WRNEQVYQQSYERGIAAGPLTMTGKTHRRGTKVRFKADARIFETLEYSFDTLAQRLRE
                     LAFLNPGLRIALDDEREGKRREFKYDGGIVSFVTHLNTNKTAVNEKPIHMRGEKDGTV
                     AEIALQWNDGYSETVYSFANNINTHEGGTHLSGFRAALTRTVNAYASKNGSAKDAGES
                     VSGDDIREGLTAVISVKLPRPQFEGQTKTKLGNTDVKGVVEAIVNDKLGAYLDENPAV
                     AKRIVSKAVDAARAREAARKARDLVRRKGALDNSALPGKLADCQERDPAQCELYIVEG
                     ESAGGSAKQGRDRRFQAILPIRGKILNVEKARFDRMLGSEEIRTMIAALGCGIGDEDF
                     DLARLRYHRVIIMTDADVDGSHIRTLLLTFFYRQLPELINRGHVFIAQPPLYRAKRGK
                     AETYIKDDRELDAFLVRRGIEGRVVRFSNGGAAVSGADLEQLVQRLIAFREQRQAASR
                     RGVSGRALSALLKFGARDRAFFASRDHAEALAAELGGDGRTVSTILDEEHSLYRLEVE
                     EGNGHARSMTVAADVVLSSEFRALAATYREIEQLSWPATVVADAAPAAADEAGGDSGA
                     DAGRRRGQAPAADAQADGPDELIDLFIAAGRRGVAINRYKGLGEMNPEQLWATTMDPA
                     SRTLLQVKAEDHTEADQMFTTLMGDQVEPRRKFIEDNALDARNLDV"
     gene            complement(10276..>10915)
                     /gene="dnaN"
                     /locus_tag="PLT35_02630"
     CDS             complement(10276..>10915)
                     /gene="dnaN"
                     /locus_tag="PLT35_02630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG28151.1"
                     /translation="LFVVQAGRMTLVATDGHRLAIVGAPREGAEKAEEVRTILPKKTL
                     SELARLLVEGEDEVRFARDENHIFFRIDGRLLVSRTIEGQFPAYERVIPKNNDKRVEF
                     ERDRFQAAIRRVALLANERSRAVKFSIEPGRVEVASQAAELGEAREQIAVEYNGPAME
                     ISFNAQYVLEFLGAVDSDVVAVEFKDDVSQTMMHPVGTTAYEYQYVVMPMRV"
BASE COUNT         1537 a         4000 c         3569 g         1809 t
ORIGIN      
        1 cgaagcgcct caccgcgtcc gccgaacgct cgaggacctt ctccgcctcg tcggcaaccg
       61 ccgggtcgct atcggccggg aactcaccaa gcttcacgaa gaggtcgtca ggggccgtct
      121 ctcggcggta ctcgagcgcc tctccccgcc caggggcgaa tacactatcg tcgtgggcgg
      181 cgaagaagag gagacaccgg acagagacga gcaagtctct gacaatcaat tgtttgaaga
      241 attcagttgt ttgacagaaa acaaccggac tcgccgcgag gccattcgcg agctcgctga
      301 aatccacggc atgcgtgcga gagacgtttt cgcggccctc gaccgcgtcc gccgtccttg
      361 accctttcgc gcgtcatttg ctaacgtagc gcagccaaga acgcctcgag cgtcaaaatc
      421 gtgacgcctg cgccagtgga gttgtggtat ggcgaagatc cggcgcggct cccgtcgtca
      481 ggccagcgtg aagaaggcca agcgggcagt ccctgcgaag acgaagcgga gtgcttcctc
      541 cgccaagcgc ccttcccggc ctgctgccag accctcgacc aggtcgtcgg ccagacccag
      601 gacggcgcct cccgccaaac catccggccg gccgtccgcc aagccggcgc cgcaggcgac
      661 tcccgcccgc cgcagcacct acgcggacgc catcatcctc tacgagcggg ggctccacgc
      721 cctgcaggcg aagcgctatc gcgaggcgtc ggccacgctc cggcgggtca tcgcggaatt
      781 ccccgaggaa atcgagctgc acgagcgggc cgagctctac atccgggtct gcgagcgcca
      841 gatggcccct cccgacgcaa cgccgaagac gcccgacgag caggtactgg ccgccacgct
      901 cgcgatgaac gccggcgcct tcgaccaggc cgccgcgctc ctcacgagcg tgctccaggc
      961 gcagcgggag cacgatggcg ccgaatacct gctcggcgtg gctctggccg cgaagggtga
     1021 cgcctccggg gccctcctcc acctggcccg ggcgatcgat ctcaatcccg agaaccgcga
     1081 tcttctgcgc aaggcgccgg aactcgaggc cctgcgccag accgacggcg gccgcgccct
     1141 gctggcggag gcgtcgagcg cggcgcgcag ggagcgccgg aaccagccgc gcgtgcggca
     1201 ttaggccggc ccgaaggccg gcgcgatcat ggtccaccag tccgtcatcg acgccctgct
     1261 tgccgcgggc gtcacgcttc tcgacccgcg caccacgttc atcgacgagg gcgtcacgat
     1321 cggcgccggg acgatcgtcc atccgggggt cactctcgaa ggccgcaccc gggtcggcgc
     1381 gcgctgccgc ctccgttcgt gggtccgcat cgcggactcc gaccttggcg acgacgtgat
     1441 cgtcaacgac tcgtgcgtga tcctcgaatc gcgcgtggac gacgggtgca gcgtcggccc
     1501 gttcgctcac ttgcggcccg gctcgcacat gaagcgcggc gcgcacgtgg gcaactacgt
     1561 cgagctcaag aagaccgtgc tcggcgaggg ctccaaggcc agccacctca cctacctggg
     1621 cgatgccgcc attggcgcga acgtgaacat cggcgccggc acgatcacct gcaactacga
     1681 cggcgcgaga aagcatccga cgacgatcgg cgacggcgcg ttcgtcggca gcggctcgca
     1741 gcttgtcgcc ccggtcgcgg tcggcgagag cgcgtacgtc gcggccggtt cgaccatcac
     1801 cgaagacgtg cctccgggcg cgctcggcat cgcgcggggc cggcagcgga acgtcgaggg
     1861 gtgggcggcg cgcaggagga ggcagacgga agccccggtc cccgcccacg attcctcggc
     1921 ctcggcgtcc cagccgtccc ttcgcggccc cgagggcggg agtccggccg aagggtcttg
     1981 acagaacgga cgcggaggcg cccgcgcacg cccgcatgga tctgctctca tccctgaacc
     2041 ctccccagcg cgaggccgtg gtgtacacgg atggcccgct gctcatcctg gccggcgcgg
     2101 gctcgggcaa gacccgtgtc atcgcccacc gcgccgccta tctggcgggc agcgggctcg
     2161 cggcgccgga atccatcctc gcggtcactt tcacgaacaa ggcggccgag gagatgcgcg
     2221 accgcgtggg cgcgctgctc ggcggcagcg ccggcggcat gtggatctcg acgttccacg
     2281 ccttctgcgc gcggctgctg cggcgcgagg ccatccgcgc gggcctctcg cgggacttcg
     2341 tcatctacga ctcgtcggac cagctggctg cggtcaagca ggtcctgcgc gatctgggcg
     2401 tcgaggacac cctcctggcc ccccgcgccg cgctcgcccg catcagccat gcgaagaacc
     2461 gcatggagtc gccggcgtcg atgcccggcc gccacgccag ccgccgcgac gaggcggccg
     2521 cccgcgtgta cgccgagtac gtcaaggtcc tcgccgcaaa cggcgctttg gacttcgacg
     2581 atctcctgct tcgcgccgtc gatctgctcg accgcgcgga gacggtccgc gaggcctaca
     2641 gccgccgctt ccggttcgtg atggtggacg agtaccagga cacgaaccgg ccgcagtacc
     2701 tgctcatgcg ccgcctggcg gagcaccatc ggaacctgac ggtcgtcggc gacccggacc
     2761 agtcgatcta tgcgtggcgc ggcgcggaca tccggaacat cctcgacttc gagacggact
     2821 tccccgaggc gaaggtcatc aggctcgagc agaactaccg ttccacgcag gtcatcctcg
     2881 acgccgcctc tgccgtcatc gcccgcaacg tcaaccgcaa ggagaagcgc ctctggaccg
     2941 atcggagcgg cggcgacctc attgtctgcg cgcggtgcgg cgacgaaatc gaggaggcgg
     3001 acttcgccgc caggaccgcc cggagagtgc tcgcggccga tccccgcgcg tccgcggcgc
     3061 tgctctaccg cacgaacgcc cagtcgcgcg cgctcgagga cgccctccgc cgggaggacc
     3121 tcccgtaccg catcgtcggg agcgtccgct tctacgagcg caaggagatc aaggacgccc
     3181 tcgcctacct caagctgctg ctcaatccgg ccgacgacct cagcctgcga cggatcatca
     3241 acacgcctgc gcgcggcata ggcaagggcg tgctggacgc cctggaagcg catcgcgccg
     3301 ccggcccggc cggcctggct ttcggcgcgc ccgccggcct cggtccgccg gccggtggcg
     3361 cgcaggcccc gatagccgcg ccggctcccg acccgctcag gtggtccctc tgggcggccc
     3421 tcgaacacgg tctcgaagcg cggcgcttcc cggcacgcgc ggcggcgtcg ctcaccgcct
     3481 ttcgcgacct catccgatcg ctcgccaaca tggccaggcg cggcccggcg tcggtcgcgc
     3541 tggcgacggt gctggatcgg accggctacc tgcaggatct tcgggatgag cgcagcgagg
     3601 aggcccaggg ccggatcgag aaccttgccg agctcgtatc ggccgcccgc gagttcgaga
     3661 gccgggaccc cgagccctcc ctggccgggt tcgtcgatcg cctgtcgctc ttgtcggaag
     3721 cggacgaatc ggaaggcgcc cgggacgcgc gcgtgctgct gatgacgctc cacgcggcga
     3781 aagggctcga gttccccgcg gtcatcgtca ccggcctcga ggaagggctg ttcccgcacg
     3841 ccaggtccgc cgacgaggac ggcagcctcg aagaggagcg acgcctctgc tacgtcggca
     3901 tgacgcgggc ccggcagcaa ctgttcctga cgagcgcggc ccgccgccgc gtgttcggtg
     3961 agtaccgccc caccgagccg tcgcgcttcc tggacgagat tccgtcgtct ctcgtccagc
     4021 gctacgagtt cgtcggcgcg ggcgcgagcc cgaccgcgcc cgacgccgcg gatttcgcat
     4081 tcaggttctc gcggcgcccc ggtcgacaag gcgcggccgg attccgctcc tcgacggacg
     4141 cctcaggcgc gccccggggc ctgacctatt cgccggagga cgaggaccag tctgcccacc
     4201 ggtcgggcgc cggcctgggg atgcgcgtca ggcatccccg gttcgggctc ggcacggtcc
     4261 tcgccatcga ggaccacgcc gacgatgtca aactgaccgt caggttcgac acggcgggcg
     4321 tgaagcggct ggtggggaag tacgcgagac tcgagcgggc gtaggggcgg gatcgcgagc
     4381 gggcgcgcga gggggcgccc gcctgcggcg ggcgcccccg tgcagtctac tccgcgtcct
     4441 tcctgcccgc caccacccag cgccccacaa gctccctgcc gttgacgccc tgcagggtga
     4501 tgcgctgcag cgtgaagacg tacttcttcg tggcgacgcc cgcgtccgtc ttgaccttgc
     4561 cctcgatggt gacatccttc gtcgtggact cgccctcgta ggccgtcgtg tcaatcgggt
     4621 tccgctggtc ctggcagctg atttcgacga tcggctgctc ggccgcgacc tgcttgcgga
     4681 tctcggagac cttcttggcg tgcgccgcgg tctcctcgcg ccacttcgtc cactccttct
     4741 ggatctccgc gtccttgccg cggagcggct ggttcttcgc ctcggccttc agcacccggt
     4801 cgatgccgtc cgcgttggcg tcctggtact ccttcttctt ctccgagaac gccttctcct
     4861 cgtcggatgc ggccttgagt tcctgggctc gcgccttgat cgtgagcggc gccacggccg
     4921 gctccgaaat gctcagcacc ttgaacgact gcatctggcc gtccaccttc ggatcgaagg
     4981 ccacggtggc gatgttggag agcgtcatgt tgtcgctcac cttggacgcc atgaagtact
     5041 tgttcaggag gatctgttcc tccccgccgc cgcaggctga ggcgacaacc gccaacacca
     5101 gagccggaac catcaaccaa cgcttggaaa taggccgcga catgccaccc tcctctacac
     5161 acccgttgcc gtttcgtcct cgcgctctgc gagccgcgcc accgacacga cgcgatcctc
     5221 cgcctcgatc tcgatcagcc ggacgccttg cgtcacccgg ccgatggccc ggataccgct
     5281 ggttggcatc cgcagcactt tcccgagctg cgtgatgagc atgagctcgt cttcgtcccg
     5341 cacgtacacc gctccggcga cgcgcccgtt ccggtcagac gtgtggatcg aaatgatacc
     5401 cactccgccg cgagattgca cgcggtattc ctccagggcg gtccgcttcc cgtatccgtt
     5461 ctcgcagacg gtcaggaccg tcccgccccc gccgggctcc acgacctcca tggcgacgac
     5521 gctgtcccct tcccggaggc tgatgccccg gaccccgtag gcggtccgcc ccatgggcct
     5581 gacgtccgtt tcggggaacc ggatggccat cccgtcgtgg ctggccagga agatctcgcg
     5641 cgttccgtcg gtcacctgga cggcgatgac ggcatcgtcc tcggcgacgc ccatggcaat
     5701 gatgccgccg gccctggggt tctggaaggc cgtcaaggag gtcttcttga cgacgccctt
     5761 ccgggtgccc atgaagacga acttgtcctc ctcccaggcc cggccgggca gcagcgaagc
     5821 gatcttctcg ccggccgcca tggagaccag gttcgcgatg gccttgccct tgccgtcggg
     5881 gcccacgtcg gggatttcgt gcactttgag ccagtaggcc cgtccccggt ccgagaacac
     5941 gaggagcgag tcatgggtgg acgcgatgag gaggtgatcg acgaagtcct cctcccggaa
     6001 gcgcatgccg atgcgcccct ttccgccccg ctgctggttc cggtagctcg tgaccgccgt
     6061 gcgcttgacg tagccggtgt gggtgaccgt gatcgccatg tcctcgtcgg cgatcaggtc
     6121 ctcgatgcgg aactcgccgc tctcgtcgat gatctcggtc cggcgcgcgt cggcgaacgc
     6181 ctgccggacg tcccggagct cctggatcac ggtggccatc agcagccgct cgtcggccag
     6241 gatggcccgc aggcgttcga tcgtctggag gacctccgcc agctcgtcct cgatcttctg
     6301 gcgctcgagg cccgtcaggc gctgcagctg catgtcgagg atcgcctggg cctggattgc
     6361 cgacagcccg aacgaggcca tcaggccttc gcgcgcctcg gccgggttcc gcgaggcgcg
     6421 gatgagggcg atgacggcgt cgaggtgatc gagggcgatc ttcaggccct cgaggatgtg
     6481 ggccctggcc tcggccttcc gcagctcgaa gcggatgcgc cgccgcacga cgtcccgccg
     6541 gaagtcggcg aactgctcga ccatctcgac gagcggcagc acgcgcgggc gcccgccgac
     6601 aatggccagc atgatgacgc cgaacgacac ctgcagcgcg gtgtgcttga agaggttgtt
     6661 caggacgacg tccggcacct ccccgcgctt caggtcgatg acgatgcgca tgccgtcccg
     6721 gtccgactcg tcgcgcaggt ccgcgatccc ctcgatggtc ttgtcgcgca ccaggtcggc
     6781 gatcttctcg atgagacgcg ccttgttcac ctggtacggg atctcccgca ctacgatgga
     6841 cgtgcggtcc cctttcttcg cggtctcgat ctccgtccgc gcgcgcacca ggatggatcc
     6901 gcgccccgtc cggtacgcct gggcgatgcc cgcgcggccg acgatgatgc cgcccgtcgg
     6961 gaaatcgggg cccggcatca ggcgccacat cgccgacagc ttctggtccg cgccggcctc
     7021 cgggtgctcg atggcgtaga tgacacagtc gacggcctcg cccaggttgt ggggagggat
     7081 gttcgtggcc atgcccaccg cgatgccggc ggacccgttg accagcaggt tggggaacgg
     7141 cgccggcagg acgccgggct cctcggtcgt ctcgtcgtag ttcggcgcga agtcgacggt
     7201 gtccttgtcg aggtccgcca tcagctcctc ggccagggcc ttcagccgcg cttccgtgta
     7261 ccgcatggcg gcgggcggat cgccgtccac ggacccgaag ttgccctgcc cgtccaccag
     7321 cgggtagcgc atgttgaagt cctgggccat gcgcaccagc gtgttgtaga tggcgctgtc
     7381 gccgtggggg tggaagttgc ccatcacctc gccgacgatc ttggcgctct tgcggtgggc
     7441 ccggcccgac cccaggccca tcaggcgcat gccgtagagc acgcgccggt gggacggctt
     7501 gaggccgtcg cgcacgtccg gcagcgcccg cccgatgatc acgctcatcg cgtaatccag
     7561 gtacgagcgc ttcatttcgt cttcgatcgc gacggggatt ctgctcgcgg tgtccattcg
     7621 gcttcgctcg gtctctccgc tcgctggttc ggaattcaga atccggaatc cgggatcctg
     7681 tccgacgtca gacgtccagg ttcctcgcgt ccagcgcgtt gtcctcgatg aacttcctgc
     7741 gcggctccac ctggtcgccc atcagcgtcg tgaacatctg gtcggcctcg gtgtgatcct
     7801 cggccttcac ctgcagcagg gtccgcgacg cggggtccat ggtcgtggcc cagagctgct
     7861 cggggttcat ttcgccgagg cccttgtagc ggttgatggc gaccccgcgc ctcccggccg
     7921 cgatgaacag atcgatgagc tcgtcgggcc cgtccgcctg cgcgtccgca gccggggcct
     7981 gtccccgccg cctcccggcg tccgcgccgc tgtcgccgcc cgcttcgtcg gcggccgccg
     8041 gggcggcgtc ggccacgacc gtcgcgggcc acgacaactg ctcgatctcc cgataggtcg
     8101 cggccagggc gcggaactcg ctcgacagca ccacgtcggc ggcgaccgtc atgctgcgcg
     8161 cgtgcccgtt cccctcctcg acctccaggc ggtagaggct gtgctcctcg tcgaggatcg
     8221 tgctgaccgt gcgcccgtcc ccgcccagct cggcggccag cgcctcggcg tggtcgcggc
     8281 tcgcgaagaa ggcgcggtcg cgggcgccga atttcagcag cgccgagagc gcgcgcccgg
     8341 acacgcctcg ccgcgacgcg gcctgccgct gctcccggaa cgcgatcagc cgctgcacca
     8401 gctgctcgag gtccgcccct gacaccgccg cgccgccgtt ggagaagcgc acgacgcggc
     8461 cctcgatgcc gcgccgcacc aggaaggcgt ccagctcgcg gtcgtccttg atgtaggtct
     8521 cggccttgcc gcgcttcgcc cggtagagcg gcggctgcgc aatgaagacg tggccgcggt
     8581 tgatgagctc cggcagctgc cggtagaaga acgtgagcag cagcgtcctg atgtgcgagc
     8641 cgtccacgtc cgcgtcggtc atgatgatga cgcggtggta gcgcagccgc gccaggtcga
     8701 agtcctcgtc gccgatgccg cacccgaggg cggcgatcat ggtgcggatc tcctcgctgc
     8761 ccagcatgcg gtcgaagcgc gccttctcga cgttcaggat cttgccgcgg atgggcagga
     8821 tggcctggaa gcggcggtcg cggccttgct tggccgatcc gccggccgac tccccttcca
     8881 cgatgtagag ctcgcactgc gccgggtcgc gctcctggca gtcggccagc ttgcccggca
     8941 gcgcgctgtt gtccagcgcg cccttgcgcc gcaccaggtc ccgcgccttc cgcgcagcct
     9001 cccgcgccct cgcggcgtcc acggccttcg acacgatccg cttcgccacc gccgggttct
     9061 cgtccaggta cgcgcccagc ttgtcgttga cgatggcctc gacaacgccc ttgacgtcgg
     9121 tgttgccgag cttcgtcttc gtctggccct cgaactgcgg ccgcggcagc ttgacgctga
     9181 tgaccgccgt cagcccctcg cggatgtcgt cgccgctcac gctctcgccc gcgtccttgg
     9241 ccgagccgtt cttcgacgcg taggcgttga ccgtccgcgt cagcgccgcg cggaacccgg
     9301 acaagtgcgt gccgccctcg tgcgtgttga tgttgttcgc gaacgagtac accgtctccg
     9361 agtagccgtc gttccactgc agcgcgatct cggccacggt gccgtccttc tcgccgcgca
     9421 tgtggatcgg cttctcgttc accgccgtct tgttcgtgtt caggtgcgtg acgaacgaga
     9481 cgatgccgcc gtcgtacttg aactcgcggc gcttcccctc ccgctcgtcg tcgagggcga
     9541 tgcgcagacc cgggttcagg aaggcgagct cccgcaggcg ctgggccagc gtgtcgaagg
     9601 agtactccag cgtctcgaag atcctcgcgt ccgccttgaa gcgcaccttc gtgccccgcc
     9661 ggtgcgtttt cccggtcatc gtcagcgggc cggcggcgat cccgcgctcg tacgactgct
     9721 ggtagacctg ctcgttgcgc cagatctcca ggtcgagagt ctccgagagg gcgttcacga
     9781 ccgacactcc cacgccgtgg aggccgcccg ccaccttgta gctcgcgttg tcgaacttgc
     9841 cgccggcgtg cagcaccgtc atcacgacct cggccgcagg cctcccgctc tcgtgcatgt
     9901 ccacgggaat gccccgaccg ttgtccacga cggtgatgga gttgtccgtg tggatggtga
     9961 cgcggatttc gtcgcagtac ccggcaaggg cctcgtcaat cgagttgtcc accacctcgt
    10021 agacgaggtg gtgcaggccc acgggtcccg tcgagccgac gtacatcgcc ggccgcttcc
    10081 ggaccgcctc aaggccctcc aggaccttga tcttgtccgc cgaatagtcg cccgcgtccg
    10141 tgcccccggg gccgacgagg ttctcttccg ccgacacccc tggcgcctct tccagccgca
    10201 tcctgtcgtc cgccgctgcc atggattccg tttctgccgc ttcgctccgg ccgggggccg
    10261 gcgcgccgcg ccgcatcaga ccctcatggg catcacgacg tactggtact cgtacgccgt
    10321 cgtccccacc gggtgcatca ttgtctggct cacgtcgtct ttgaactcga ccgcgaccac
    10381 gtcgctgtcc acggcgccga ggaactcgag cacgtactgc gcgttgaagg aaatctccat
    10441 ggccggcccg ttgtactcca ccgcgatctg ctcgcgcgcc tcgcccagct cggcagcctg
    10501 cgacgccact tcgacccgcc ccggctcgat ggagaacttc accgcgcgcg acctctcgtt
    10561 cgcgagcagc gccacccgcc tgatggccgc ctggaagcgg tcccgctcga actccacgcg
    10621 cttgtcgttg ttcttgggaa tgacccgctc gtaggccggg aattgcccct cgatcgtgcg
    10681 cgacacgagc agccgcccgt cgatgcggaa gaagatgtgg ttctcgtcgc gcgcgaaccg
    10741 gacctcgtct tcgccctcga cgagcaggcg agccagctcg gacagcgttt tcttcggcag
    10801 gatcgtgcgg acctcctcgg ccttctcggc gccctcgcgc ggcgccccca cgatggccag
    10861 gcggtgcccg tccgtcgcga ccagcgtcat ccgcccggcc tgcaccacga acagc
//
LOCUS       DAOSIF010000040        10831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5971_length_10831_cov_4.663604, whole genome shotgun sequence.
ACCESSION   DAOSIF010000040 DAOSIF010000000
VERSION     DAOSIF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5971_length_10831_cov_4.663604"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="PLT35_02635"
     CDS             complement(<1..1586)
                     /locus_tag="PLT35_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28152.1"
                     /translation="MRSARLAPAAALAAACAGAAAWACCGTLALTEEGARAARVGLLP
                     PWWWLIALAAGAVALAWLGRLPSDRARPLFFSLVLVLPWLPVRLPPAALAWTGPASWL
                     VWAAIGGAMCAGGGQSAGRRAWRVPGFDDPARAPWIAFVCAAIVYGAAAHRLAPLVPG
                     GDEPHYLVIAQSLWRDGDLKIENNHQRGDYLEYFGGALRPDYLKRGKDGQIYSIHLPG
                     VPALVAPALAAGGHRLVMALLVLVSAGATAAAWRAACLVTGSVAAAWFGWAAAALTAP
                     FLLLSFTVYPDGPGAAVVMLAFAALVGLDVRAGRPVAWWLALGALPAALPWFHPRFAV
                     LAGTLGLVFVARALGERNRAAAVGAFAVVPAASALGWFAYYYAIYGQLSPAAAYGHYT
                     QMTLGRVPVGVLGLLADQQFGLLATAPVFSVAIAGLAALARLRRRLALEWLLVVAPYV
                     LVTAAYHMWWGGLASPARFAGATLLLFSLPAAAAWNAARHSASRQTAAIALSVSMALT
                     ALIVCAERLSGFKRPREVRVV"
     gene            1613..3262
                     /locus_tag="PLT35_02640"
     CDS             1613..3262
                     /locus_tag="PLT35_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28153.1"
                     /translation="MRSRAREAGCVILAAAAITAVMTWPLAPRAGSAGRVDSGDGAFS
                     VWNVAWVARTLVVAPRGLYDANIFYPRRGTLAYSEANLGAGVLAIPFYWASGGNAYVA
                     HNAVVLLAFVLALTGTYSLVRFLTRHRGGAAVAAVAYTFCPYIFSHIPHIQLLMHAGI
                     PYALLAFHRFADRQTAGRALVLALALAAQALSCGYYGIFAGLLVAFGALFYGISRGLW
                     RNWRYWAGLAAAAGVSVAIVLPFFLPYIELQKEAGFVRSLDDSRRWSALWRSYLASGA
                     RAHRWLLPYLGTWGEVLFPGMVVTVLGVAGLAARRRAARAAAAAGAHIWFYASVAVIA
                     FWASFGPDAGLYAWLYRVVPVFAWLRAPSRLGLLVVLALAVLAGFAVSALLARSRRAA
                     LLSTALVALTMADVCVAPMFLVDARPVSPVYDSLRKWPGGPVVEFPFFYERMDFSRHC
                     EYMLYSTAHWKPLINGYSDYIPPEWRQMVVPLSSFPNEESFKILKPLRARYAVFHFNL
                     YDHRQAAVVKQRIEQFREYLRPIRIEDQVWLFQIVAWPPAH"
     gene            3223..4845
                     /locus_tag="PLT35_02645"
     CDS             3223..4845
                     /locus_tag="PLT35_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28154.1"
                     /translation="MAVPDRRLAAGALAACLALAALHTWPLAANPGGLSRHDNADTQL
                     NEWIVAWVAHQAPRAPLRLFDANIFHPEPRSLAFSEHMTVQGLMALPVFALGGSPVLA
                     YNLLVLAGLALSAWAMWLVVRRWTGSHAAGAVAGCLAAFNAHTLTRLPHLQALHVEFL
                     PVVLLAADRLLVRRRTRDAILLGLAAALQSLTSNYLMAATVVAGAAILAARVGEVARP
                     APRRVLLLFALATAVGAALVAPFLLPYAAARREQGLVRTLDEVARYSSTWRDYVSTAG
                     RFHYAWWSHRFFEGSTALFPGVTAVLLAGVGLVAAPGWRDGRVRMAAAIGLAGAALSF
                     GPALPGYAALHRWLPLLQGVRGAARFGFLALVAVAVLAGFGVAAIGARARSRRWWPAF
                     AAALLLLVNAEALRAPIAFRPFAGIPRIYQSLRDPSVRAVAEFPFATPATIARNAPYV
                     LSSTAYWKPLVNGYSGFVPRSYADTAEALRAFPDETSRARLLSLGVTHVVVHADAYGS
                     RAPALLAALAAAPWLGLVASEGGVRVYRLNAS"
     gene            complement(4832..6913)
                     /locus_tag="PLT35_02650"
     CDS             complement(4832..6913)
                     /locus_tag="PLT35_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG28155.1"
                     /translation="MQDYWLVPPPGWAPAPPDAVEAARALARAAAYIAEEKPAQALPF
                     IRRTALAATPLAPYAVYLKGMAELGLERYEQARRTFESLRASPHPGYLTEAVALQLAE
                     VNSTLGSHAAAAALYDEVLAGTPAAADDVLLRLARAASRAGDRARALTAYESLYYDWP
                     ASEAADAAAEELPAGEMAPLEAGSPRLARELARGERLFEERRYGPAREAFERLAGLAS
                     GDAAELVALRLAECDYFLRRYQRVREDLKPWLTSASRRDEARYFDLMAARAAGKRADF
                     IALTRALVTDFPASAWAEDALNALASTYAAADEDENADAVYREIASRFPSGRYTARAQ
                     WKMGWRAYRQRRFAEAANVFEAAAVAFPRSDFRPSWLYWAAKARSRLDGRPGADDAFR
                     LVVADYGSSYYGRLAAEALAARPSAPPAAGRAAPARAAGEALADGAAPPTASLIRWLV
                     SVGMYYEALDEVRYAERTWGASPLLAATRAWLLNRTGVLRPAINLMRRTYPQFLTAGG
                     DAMPREILEIIYPLDHWALLRRYAGLHKLDPYLVAALVAQESTFERDIVSPAKAIGLM
                     QVLPTTGRQWAARLGIRGFSRQKLTVPEVNVRIGTAYLASLVARFGSEHLALAAYNAG
                     ESRVARWLRERPGLPQDEFIDDIPFPETQNYVRRILGTAVDYRKLYAAREPGSVPAAS
                     ATRKPASLKKR"
     gene            complement(7078..9078)
                     /locus_tag="PLT35_02655"
     CDS             complement(7078..9078)
                     /locus_tag="PLT35_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28156.1"
                     /translation="MTLEEQIKHAIEAAAAQLAGQFQERLQALASDVGRSAAAEHARA
                     VDEAVAAVRARLDQERETALAALRAETTEQRELAVSMAREEAAEQRAAAVAAARAEVA
                     NEHAAALAALKAEADAQAAASLEALTSELSEQHAAELAALRSQLTLEHEATIADLRAE
                     LAGRHDAALAALRAELAGQHDSALAAARAEAAEQRDLAVSMARTDAAEQRDAALAEAR
                     VEFSRERDAALAALRADLAAQHESALASVRRELAGQHDTALAAALATAIASARAELVS
                     GHESELAAIRAEAAGERERLEAAAAAENERLRGQHEAAITALRTEADAEIARARSEAE
                     AALAAAQADADRRLAAAQAEAEASLIRTIEAAKAEVALEAGRAAEDARASERAAERAA
                     TERIADGVRRLDASRSLTEIFDVIVDAASREAPRAAVFVVKGHTLTGWRAAGFTRDED
                     PRETELPRDQAGLLTAALRSGAAANTADAIAAEAGASPFGALPAASVGLAVPLLVGGD
                     AVAVLYADDVSSRERHTPSAWPESVELLARHAARCLEVLTVSRVTQAVSIPAPSRPTP
                     APRRHTPQARVFQAGHDDDDDAARRYARLLVSEIKLYHESAVTAGRRDRNLRERLRTE
                     IDRARRLYDERVPAPILARTDFFEQELVRTLANGDESLLGPV"
     gene            9284..9639
                     /gene="ssrA"
                     /locus_tag="PLT35_02660"
     tmRNA           9284..9639
                     /gene="ssrA"
                     /locus_tag="PLT35_02660"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10110..10831)
                     /locus_tag="PLT35_02665"
                     /pseudo
     CDS             complement(10110..10831)
                     /locus_tag="PLT35_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903157.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1383 a         4025 c         3855 g         1568 t
ORIGIN      
        1 accacgcgca cctcgcgcgg ccgcttgaag cccgacaggc gctcggcgca gacgatcagc
       61 gccgtcaggg ccatgctgac cgacagcgcg atggcggccg tctgccggct ggcgctgtgc
      121 cgggccgcgt tccacgcggc cgccgccggc aggctgaaca gcaggagcgt cgcgcctgca
      181 aaccgcgccg gcgacgcaag ccccccccac cacatgtgat aggcggccgt gaccagaacg
      241 tacggcgcga cgacgaggag ccactcgagg gcgagccgcc ggcgcagccg ggcgagcgcg
      301 gccaggcccg caatcgcgac gctgaacacc ggcgcggtcg cgagcagccc gaactgctgg
      361 tcggcaagca ggccgaggac accaaccggg acccggccga gcgtcatctg cgtgtagtgg
      421 ccgtacgccg ccgccggact caactgccca tagatggcgt agtagtaggc gaaccagccc
      481 agcgcgctcg cggccggcac gacggcgaac gcgccgacgg ccgccgcgcg gttccgttcc
      541 ccgagagcac gcgcgacgaa gacgagcccg agcgttcccg cgagcacggc gaacctcggg
      601 tggaaccacg ggagcgccgc cgggagggcg ccgagcgcga gccaccacgc caccggccgt
      661 cccgcccgga cgtcgagccc gacgagcgca gcgaacgcca gcatgacgac ggcggcgcca
      721 ggaccgtccg gatacacggt gaacgacaga agcaggaagg gcgccgtgag cgcggcagcc
      781 gcccatccga accaggccgc cgcgacgctg ccggtcacca ggcatgccgc ccgccacgcc
      841 gccgccgtcg cgcccgcgga aaccaggacg agaagcgcca tgacgagacg gtgcccgccc
      901 gctgccaggg ccggcgcaac cagggccggc acgcccggaa ggtggatcga gtagatctgg
      961 ccgtccttgc cccgcttgag atagtccggc ctgagcgcgc cgccgaagta ctcgaggtag
     1021 tcgccccgct ggtggttgtt ctcgatcttc aggtcgccgt cgcgccacag gctctgcgcg
     1081 atcacgaggt agtgcggctc gtcgccgccg gggacgagcg gcgccaggcg gtgcgcggcc
     1141 gcgccataga cgatcgcggc gcacacgaaa gcgatccagg gcgcgcgcgc ggggtcgtcg
     1201 aatcctggaa cgcgccacgc acgccggccc gcactctggc cccctcccgc gcacatcgca
     1261 ccgccgatcg ccgcccacac cagccacgag gcaggacccg tccacgccag cgcggcggga
     1321 gggagccgga cgggcagcca cggaaggacc aagacgaggc tgaagaacag cggccgcgcc
     1381 cgatcggacg gaagccgtcc gagccacgcc agcgcgacgg cgccggccgc gagcgcgatg
     1441 agccaccacc acggcgggag aagcccgacg cgagccgcgc gcgcgccttc ctcggtgagg
     1501 gcgagcgtgc cgcaacaggc ccacgcggcc gcgccggcgc acgccgcggc gagcgcggca
     1561 gccggcgcca gtcgagctga gcgcatgggc cgccatgcta ccatacggcc ccatgcgttc
     1621 ccgtgcgcgc gaggccggct gcgtcatcct cgcggcggcc gcgatcaccg cggtcatgac
     1681 ctggccgctc gccccaaggg ccggctcggc gggccgggtc gattcgggcg acggcgcgtt
     1741 cagcgtctgg aacgtcgcct gggtggcgcg cacgctggtc gtggcccccc ggggcctcta
     1801 cgacgccaac atcttctatc cgcgccgggg caccctggcg tactccgagg ccaacctcgg
     1861 cgccggcgtc ctggcgattc cgttctactg ggccagcggc ggcaacgcct acgtcgcgca
     1921 caacgccgtc gtgctcctcg cgttcgtcct ggcgctgacg gggacgtaca gcctggtcag
     1981 gttcctcacc cggcaccgcg gcggcgcggc ggtggcagcc gtcgcctaca cgttctgccc
     2041 gtacatcttc tcgcacatcc cgcacattca gttgctcatg cacgccggca tcccctatgc
     2101 gctgctggcg ttccaccggt tcgcggatcg gcagacggcc gggcgggcgc tcgtcctggc
     2161 gctggcgctg gcagcccagg ccctgtcgtg cggctactac gggatcttcg cgggcctgct
     2221 cgtcgccttc ggcgcgctct tctacgggat ctcccggggc ctctggcgga actggcgcta
     2281 ctgggccggt cttgcggccg cggcaggcgt gtcggtcgcg atcgtgctgc cgttcttcct
     2341 gccgtacatc gagctgcaga aggaagccgg gttcgtccgg tccctcgacg actcgcggcg
     2401 gtggtccgcg ctctggcgtt cgtacctggc ttctggcgcc cgggcccacc gctggctgct
     2461 gccgtacctc ggcacctggg gcgaggtcct ctttccgggg atggtcgtca cggtcctggg
     2521 cgtggcggga cttgccgccc gtcggcgcgc cgcgcgggcc gctgcggcag ccggcgcgca
     2581 catctggttc tacgccagcg tcgccgtcat cgccttctgg gcttcgttcg gccccgacgc
     2641 gggcctttac gcctggctct accgcgtggt gccggtcttc gcctggcttc gcgcgccgtc
     2701 gcgcctcggc ctgctcgtcg tgctggccct tgccgtgctc gccgggttcg ccgtctccgc
     2761 cctgctggcg cgctcccggc gagccgcgct cctctccacc gcgctcgtcg ccctgacgat
     2821 ggccgacgtg tgcgtggcgc ccatgttcct cgtggacgcg cggccggtgt cgccggtcta
     2881 cgactcgctg cgcaagtggc cgggcgggcc cgtcgtcgag ttcccgttct tctacgagcg
     2941 catggacttc tcgcggcact gcgagtacat gctgtactcg acggcgcact ggaagccgct
     3001 catcaacggg tacagcgact acatcccgcc cgagtggcgc cagatggtcg tgccgctcag
     3061 ctcgttcccc aacgaggagt cgttcaagat cctcaagccc ctgcgggcgc gctacgccgt
     3121 ctttcacttc aatctctacg accatcggca ggccgcggtg gtgaagcagc ggatcgagca
     3181 gttccgggag tacctgcggc ccatccggat cgaggatcag gtgtggctgt tccagatcgt
     3241 cgcctggccg ccggcgcact agccgcctgc ctcgcgctcg cggcgctgca cacctggccg
     3301 ctggccgcga atcccggcgg gctgtcgcgc cacgacaacg cggacacgca actgaacgag
     3361 tggatcgtgg cctgggtggc gcaccaggcg ccgcgggcgc cgctcaggct cttcgacgcg
     3421 aacatcttcc atcccgagcc gcggtcgctc gccttctccg agcacatgac cgtccagggc
     3481 ctcatggccc tgccggtgtt cgcgctcggc ggctcccccg tcctcgcgta caacctgctc
     3541 gtcctggcgg gcctggcgct ttccgcgtgg gccatgtggc tcgtggtccg ccgatggacg
     3601 ggcagccacg ccgccggcgc ggtggcggga tgcctggccg ccttcaacgc gcacaccctg
     3661 acgaggctcc cccacctgca ggcgcttcac gtggagttcc tgcccgtcgt gctgctcgcg
     3721 gcggaccgtc tgctggtgcg ccgcaggacg cgcgacgcca tcctgctcgg cctcgcggcg
     3781 gctctccagt cgctgacgtc gaactacctg atggcggcga cggtcgtcgc cggggcggcc
     3841 atcctggccg ctcgcgtcgg cgaggtcgcg cggcccgctc ctcgccgggt cctgctgctg
     3901 ttcgccctcg cgaccgccgt cggcgcggcg ctcgtcgcgc cgttcctcct gccctacgcc
     3961 gccgcccggc gcgaacaggg gctcgtccgg acactggacg aggtggcgcg ctattcctcg
     4021 acatggcggg actacgtctc gacggcgggc cgattccact acgcctggtg gagccaccgg
     4081 ttcttcgagg gatcgacggc gctgttcccg ggcgtcacgg cggtcctcct ggccggtgtc
     4141 ggccttgtcg cggcacccgg ctggcgagat gggagggtgc ggatggcggc ggccatcggc
     4201 ctcgccggcg cggccttgtc gttcgggccc gcgctgcccg gctacgcggc gctgcaccgc
     4261 tggctgccgc tgctgcaggg ggttcgcggc gcggcccgct tcgggttcct ggcgctggtg
     4321 gccgtcgccg tcctcgcggg gttcggcgtg gcggccatcg gcgcgcgggc ccgcagccgc
     4381 cggtggtggc cggcgttcgc ggcggccctc ctcctgctgg tcaacgccga ggccctgcgg
     4441 gccccgatcg ccttcaggcc gttcgccggg atcccgcgga tctaccagtc gctgcgcgat
     4501 ccgtcggtcc gcgcggtcgc cgagttcccg ttcgccacgc ccgcgacgat cgcgcggaac
     4561 gcgccctacg tgctcagttc caccgcctac tggaagccgc tcgtgaacgg ctacagcggc
     4621 ttcgtgcccc ggagctacgc ggatacggcc gaggccctgc gcgcctttcc agatgagacg
     4681 tctcgggcgc gcctcctttc gctcggcgtc acgcacgtgg tcgtgcacgc cgacgcgtac
     4741 ggctcccggg cgccggccct gctcgccgcg ctggcggccg cgccgtggct cggcctcgtt
     4801 gcgtccgaag gaggggtccg ggtctaccgc ctcaacgctt cttgagggag gcgggctttc
     4861 gcgtggccga agccgcgggc acggaaccgg gttcgcgtgc ggcgtacagc ttccggtagt
     4921 ccacggccgt gcccaggatg cggcgcacgt agttctgggt ctccgggaac gggatgtcgt
     4981 cgatgaactc gtcctgcggg aggccggggc gttcgcgcag ccaccgcgcc acgcggctct
     5041 cgcctgcgtt gtaggcggcc agcgccaggt gttcgctgcc gaaccgcgcg acgaggctcg
     5101 ccaggtatgc cgtgccaatg cggacgttca cctccggcac ggtcagtttc tgccgcgaga
     5161 acccccggat gcccagccgc gctgcccatt ggcgcccggt cgtcggcagg acttgcatga
     5221 gcccaatcgc cttcgccggc gatacgatgt cgcgctcgaa ggtggactcc tgggcgacga
     5281 gcgcggccac gaggtacgga tcgagcttgt gcaggcccgc gtaccggcgc aggagcgccc
     5341 agtggtcgag gggatagatg atctcgagga tttctcgcgg catcgcgtcg ccgccggccg
     5401 tgaggaactg ggggtaggtt cgcctcatca ggttgatggc cggcctgagc acgcccgtgc
     5461 ggttgagcag ccaggcgcga gtggcggcga gcagaggcga tgcgccccac gtccgctcgg
     5521 cgtagcgcac ctcgtcgagg gcctcgtagt acatcccgac ggagacgagc cagcggatga
     5581 gactggcggt tggcggggcg gcgccgtctg ccagcgcctc gcccgccgct cgagccggcg
     5641 cggcgcgccc ggccgcgggg ggcgctgacg gccgggccgc cagggcctcg gccgcaagtc
     5701 ggccgtagta cgagctgccg tagtctgcga ccaccagcct gaaggcgtcg tccgcgccgg
     5761 ggcggccgtc gagccggctc cgcgccttgg ccgcccagta gagccacgag ggacggaagt
     5821 cggaccgcgg gaacgcgacg gcggcggcct cgaacacgtt cgcggcctcc gcgaaccggc
     5881 gctgccggta ggcccgccat cccatcttcc actgggcccg cgccgtgtac cggcctgacg
     5941 ggaagcgcga cgcgatctcg cggtagaccg cgtcagcgtt ctcgtcctcg tcggccgcgg
     6001 cgtaggtgga ggcgagggcg ttcagggcgt cctcggccca cgcgctcgcc gggaaatcgg
     6061 tcacgagggc ccgcgtcagc gcgatgaagt ccgcgcgctt gccggcggcc ctggcggcca
     6121 tcaggtcgaa gtagcgggcc tcgtcgcgcc gcgaagccga ggtcagccac ggtttgaggt
     6181 cttcgcggac gcgctggtac cgccgcagga agtagtcgca ttcggcgaga cgcaacgcca
     6241 ccagctcggc cgcgtcgcct gaggcgagcc ccgcgaggcg ctcgaaggcc tcgcgcgcgg
     6301 gcccgtaccg ccgctcctcg aacaggcgct cgccgcgcgc cagctcacgg gccaggcgcg
     6361 ggctccccgc ttccagcggg gccatctcgc cggccggcag ctcctcggcc gccgcgtcgg
     6421 ccgcctcgct ggccggccag tcgtagtaga gcgactcgta cgcagtcagc gcgcgcgcac
     6481 ggtcgccagc gcgcgaggcg gcgcgcgcga ggcggagcag cacgtcgtcg gccgccgcgg
     6541 gcgtcccggc gagcacctcg tcgtagagcg ccgccgccgc cgcatggctg ccgagagtcg
     6601 agttgacttc ggcaagctgc agggcgaccg cctcggtcag atagcccgga tggggcgagg
     6661 cgcgcagcga ctcgaacgtg cgccgtgcct gctcgtagcg ttccaggccc agctccgcca
     6721 tgcccttgag gtacaccgca taaggcgcaa gcggcgtcgc cgccagcgcg gtccggcgga
     6781 tgaacggcag cgcctgggcg ggcttttcct cggcgatgta cgccgccgcg cgggccaggg
     6841 cccgggccgc ttcgaccgca tccggcggcg cgggcgccca gcctggtggc ggcaccagcc
     6901 agtagtcctg cacgctcgcc ggaagcggcg ggtgctcggt cggccggggc tttccggcga
     6961 gcgccgcgtc ctgcccggct ggctccggac gcgcagccgg cgctgacgcc gcggcggcaa
     7021 gggcgagtgc gaggccgaga aggaggcgcc gccgtgcgcg ggcagtcccc cgcgggttca
     7081 caccggaccc agcagcgact cgtcgccgtt tgccagggtg cgcacgagtt cctgctcgaa
     7141 gaagtccgtg cgcgccagga tcggagcggg gacgcgctcg tcgtacaagc ggcgggcgcg
     7201 gtcgatctcc gtgcgcagcc gctcgcggag gttccggtcc cgccggccgg cggtcaccgc
     7261 ggactcatgg tacagcttga tctcggacac gaggagcctg gcgtagcgcc gcgccgcgtc
     7321 gtcgtcatcg tcgtggccgg cctggaagac ccgggcctgc ggcgtgtggc gccggggagc
     7381 cggcgtcggg cgggacgggg cgggaatcga caccgcctgc gtcacgcgag agaccgtgag
     7441 gacttcgagg cagcgcgccg catgccgcgc gagcagctcg acgctctcgg gccaggcgct
     7501 cggcgtgtgc cgctcccggg acgagacgtc gtcggcgtac agaactgcca ccgcgtcgcc
     7561 gccgacgagt agcgggacag ccaggcccac gctggcggcg ggcagggccc cgaagggcga
     7621 cgccccggcc tccgccgcaa tcgcgtctgc cgtgttcgcc gccgcgccgg atcgcagggc
     7681 cgccgtgagg aggccggcct ggtcgcgcgg cagctccgtc tcgcggggat cctcgtctcg
     7741 agtgaagccg gcggcgcgcc accccgtcag cgtgtgcccc ttgaccacga acaccgcggc
     7801 gcgcggcgcc tcacgggacg cggcgtccac gatgacgtcg aagatctcgg tgagcgaccg
     7861 ggaagcgtcg agccggcgca cgccgtcggc gatgcgctcg gtggccgccc gttcggccgc
     7921 ccgctcggaa gccctcgcgt cctcggcggc ccggccggcc tcgagggcga cctccgcctt
     7981 cgcggcctcg atcgtgcgga tcaggcttgc ctccgcctcg gcctgagcgg cggcgagccg
     8041 ccggtccgcg tccgcctggg cggccgccag ggcggcttcg gcctccgacc gcgcccgggc
     8101 gatctccgcg tcggcctcgg ttcgcagcgc cgttatcgcg gcctcgtgct gcccgcgcag
     8161 acgctcattc tcggcggcgg ccgccgcctc gaggcgctcg cgttcgccgg cagcctccgc
     8221 gcggatcgcc gccagttccg actcgtggcc cgacacgagc tccgcgcgcg ccgaggcgat
     8281 ggccgtggcc agcgcggcgg cgagcgccgt atcgtgctgg cccgcgagtt cgcgccggac
     8341 cgacgccagg gccgactcgt gctgggcggc gaggtcggct ctcagcgccg cgagggccgc
     8401 gtcccgctcg cgcgagaact ccacgcgcgc ctccgccagg gccgcgtcgc gctgctcggc
     8461 ggcgtcggtc cgcgccatcg acaccgccag gtcgcgctgc tcggctgcct cggcccgcgc
     8521 ggccgcgagc gccgaatcgt gctggcccgc cagttccgcg cgaagcgcag ccagggccgc
     8581 gtcatgccgc cccgctagtt ccgcacgcag gtccgcgatc gtcgcctcgt gctccagcgt
     8641 cagctgagac ctgagcgccg ccagttcggc cgcatgctgc tccgagagct ccgacgtgag
     8701 cgcttccagc gacgccgccg cctgcgcatc cgcttccgct ttcagcgcgg caagcgcggc
     8761 cgcgtgttcg ttcgcgacct cggcccgcgc ggccgcgacg gcggcggcgc gctgttcggc
     8821 ggcttcctcg cgcgccatcg agacggcgag ttcccgctgc tctgtcgtct ccgcgcgaag
     8881 ggctgcgagg gcggtttcgc gctcctggtc gaggcgggcg cgcaccgcgg caacggcttc
     8941 gtccacggcc cgcgcgtgct cggctgctgc cgaccgcccg acatccgagg cgagcgcctg
     9001 gaggcgctcc tggaactggc ccgccagctg ggccgcggcc gcctcgatcg cgtgcttgat
     9061 ctgctcctcg agtgtcatgc ctgtcgcctc gcgcggtggg atcttcggct ggcggcatta
     9121 tgccacacgg ggccgcgggc gggcgggagg cggaatggcg gtggatgcaa gccgtgtcgg
     9181 gccaccgagc gccgccgcgg ccaggcggct ttgaccgccg ggcgaagggc tcgtacaata
     9241 acgaggtgcg agccggtcag ccggctggcc tttgtttcga cgtgggggcg attctggatt
     9301 cgacggaggt gctggatcgg aggatgcgtg ccgagctcct gctgactcgt taaaccggcg
     9361 ggaacaaacc taactgcgga cacgcagctc gctctcgcgg cttaactaac cgcgacgtcc
     9421 acctggttcg ggcctgcacg gccgggctgg atgtcatctt cgcaggctag ctggtgcgcg
     9481 cggttccgac gcgcggcggc gaatcgatct cggagctagc gtccggcggt ttctcgccgc
     9541 tctctcacgc cgggcgcgaa cctacaagag cggacacgca cgtagagtcc tccggcgacg
     9601 cgctttcgga cgcgggttcg actcccgccg cctccaccac ccccctcccc aggcatcaca
     9661 aaacagcccc aaatctcccg ttattgcgtg tcattgcttc aattcgcacg gttgtcgtct
     9721 gactgggatt cccacgcaac cctttggcat accaacacga gtgttgggat cttcacccct
     9781 aaagtaccaa caacggaagt cagatgattg acgacaacta gaatcgccgg aagagcagtg
     9841 gacatcagtc agaggatcct tgtgaagccc gttcgccgtc gcctgtcgag gtcgtgtccc
     9901 gtgaagtggt ggaaactgaa tcgcgtgtgt tcctgagttc cgaagtcacc gtcggtgtgg
     9961 acgctgacgc gcccgcggac gggacgagca ccgcccgccg acgcttggag aacccgccga
    10021 cgggtttccc acagcgcccc ccgttggtca tccgttcacg caatcgccat caagacctcc
    10081 tgctcgccac ccgtccccag cggcgtgagc acccgccgca tcgactcggc actgaagtaa
    10141 cggcgttccg cgacggccca ttcctcgtcc tgctccagga ggatggcccc gaccagccgc
    10201 ggcgtcgcgc ggacgttggg gaagatgccg acgacattcg accggcgctg ggtctccttg
    10261 ttgagccact ccagcggatt cgtgctgtgc agctgccgcc gatgctcttc cagaaggtgc
    10321 ttgtgcgcta ggatgtcctc ggcggcctcc ttgagcaggg ccgcagtctg gggcgaccgg
    10381 gcgcggagtc cgtcggcgac cttccggcac ggcgtcatag cgctcccgtg atcgggcggc
    10441 gcgaagatgc tccgcacgat cgcggcgctc ggctcgcgcc tggcctgcgg caccgtcgcc
    10501 agtagattcc ccatgaagtg gacgcggcag cgctgccagg tcgccccgct cagcacggcg
    10561 ccaatcgccg ccttcagccc ttcatgggcg tcggagacca ccagtcggac ctccgccaag
    10621 ccccgcttca ccagactccg cagaaacgcc ggccagaagc cgcaatcctc cgaggcgccg
    10681 acatccacgc cgagcacggg ccgctcccca tcgagcgtca cgccgatggc cacgaccgtc
    10741 gcctagctca ccacgcggcc gtcgatgcgc acctagtggt acgtcgcatc cagccagacg
    10801 tacggattct cggtgggcag ggggcgtggg c
//
LOCUS       DAOSIF010000041        10807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_5986_length_10807_cov_4.320126, whole genome shotgun sequence.
ACCESSION   DAOSIF010000041 DAOSIF010000000
VERSION     DAOSIF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5986_length_10807_cov_4.320126"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLT35_02670"
     CDS             <1..935
                     /locus_tag="PLT35_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28157.1"
                     /translation="DRHFSSLLKDHHDIVRVLASAFDMATLPNRAELRLRPSGKVIGY
                     TLSLVRDERGRPYGAALFFKDLTLVEQAEERDRLRDRLATLGEMAAMIAHEVKNPLAS
                     IEVMAGLLRRQVPDREDLRELATDIINEAKMANAIVVEVLEFVRPIRLQVDRTSVPKV
                     IEEAVPLAESKVPRRATRLEIDVPGGLPAIQGDHHQLVQVVTNLLINALEALDGGGTI
                     RVGARLVPEDHDGSAAVELVIEDDGPGVPGDITSRIFNPFFTTKPQGSGLGLPIVRKI
                     VDAHDGRIDVATSAGRGTTFTVTLPVGVPGQLGF"
     gene            1012..2415
                     /locus_tag="PLT35_02675"
     CDS             1012..2415
                     /locus_tag="PLT35_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG28158.1"
                     /translation="MARILIADDHDALRRALARALVDAGHEVEEAANGNLAIERLHEG
                     SFDVVLSDLKMGGSDGIDVLRSTKALHPSAAVILMTAFGSVGTAVEAMKIGAFDYVQK
                     PFEIEEMEIKIGKALELRRMRHEIDYLRHTQHDIYTFDSIVGDSEALRKVLAVVGKVA
                     RSNTTILIHGETGTGKELVAGAIHHNSARSGRNFVKVNCAALQENLLESELFGHEKGA
                     FTGADKQRIGRFEQADGGTMFLDEVGDMSPSTQAKILRVLQEHEFERLGGTRTLKVDV
                     RLIAAANRSLPAMVAAGQFREDLYYRLNVVSVEMPPLRERKEDIAALADFFVRRFSGE
                     LKKKIDGIAPDALKLLMRYNWPGNIRELQNALERAVLLTDGHVITADDLRLGETTSFG
                     SDADAARVVRVPPAGIALQEIERQALVESLKMCNWVQKDAAELLRVSPRVINYKIKTL
                     GIKLPRGRGAAPGQNDG"
     gene            complement(2424..3218)
                     /locus_tag="PLT35_02680"
     CDS             complement(2424..3218)
                     /locus_tag="PLT35_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28159.1"
                     /translation="MTRRLLVRCGLALLAGLALPGLLQAGEVKLAIRDGRVTLSARDA
                     PLRQILAEWERVGGTRIFNRDRTPATPMTIELVDEAEGRALATLLRAVAGYAAAERLD
                     PAAGVSRYSRIVIMPGEAAAWASAASQPAAPSGSAFGGGGMGPGGGVGMRGPMQRRVL
                     PDGRVVLGISDDQGEPSDDADLAAPGGPPPGGMMRPPFQGPSRSAQRQPADGDEPTRI
                     DIGQERGAPAMPTYVPAPAPGMSLTAPGVVPGTRPPPSAPIRPPRD"
     gene            complement(3309..3758)
                     /locus_tag="PLT35_02685"
     CDS             complement(3309..3758)
                     /locus_tag="PLT35_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOG28160.1"
                     /translation="MIADRLANAHRYLALGPRFARAFDYLTRTDLAAAAEGRADIDGA
                     SLYAMIQRYTSKLPQDGVWEAHRVYADLQYMVSGEERFGVGAIERFTRGAYDAERDFE
                     ALAGDGDFLRIQPGRFLLLWPGEPHMPGMAIGAPAPVQKVVVKIRVD"
     gene            4121..5362
                     /locus_tag="PLT35_02690"
     CDS             4121..5362
                     /locus_tag="PLT35_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOG28161.1"
                     /translation="MNPADIAPVAPGDRRQSPLDLFVIFAGANIVATTLQVGAALAAD
                     FGAIAAVALIAAGAVGGSALVASLSVLGPRLGVPSVIAARAALGVRGAALVAGLLYVT
                     NFAWIAVNNVIAASVGSQILGGPGAMPIWVVGLGLVSTAVVALGPRAVGLADRVAVPL
                     LLLVGLIITWAVVQAPPAPVAMRQPPAAPAHWLRGFDIVVGYQVSWILMFADFSRYTT
                     SERAGAAAVFFGLLLTSLWMMPLGLAAARVAHSADPGTMMAAVGLGWAGAALMVLATI
                     TTNFVNIYMSGLAWKSLFPRAGAQSAVWSVGIVGTLFGLCYGTWLDRYAEYMVVLGGI
                     LVPVGGVLVAHFRLSRTTTHVPDLYDPAGPCRGVLLPGVIAWALGAFAYYVASSWGGT
                     LPALATAVVSYLLLRRRLAWR"
     gene            complement(5744..6721)
                     /locus_tag="PLT35_02695"
     CDS             complement(5744..6721)
                     /locus_tag="PLT35_02695"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOG28162.1"
                     /translation="MKGHLKAFLVHLRDNRGASPHTVRAYEGDLSQFIGFLPIHNGKA
                     ARDQRPSDCDVAAIRSFLAELYRTRRSRKTAARKLASVRAFVRYLRRESLIDGDPGSL
                     VSSPRLDAKLPAHLEIDEMTRLLETPNEGTALGRRDRAILELLYASGLRLGELVGLDL
                     DHVNLGGRMVRVLGKGGKERVVPFNTSAEQAIRAYLRDRPDLERAAARQRSGPARPAP
                     RSGAGRPAPRRTAGSGPLFLNFRGGRLTGRSVHRLVRRYVAACSARLGVSPHALRHSF
                     ATHLLERGADLRSIQELLGHAQLSTTQRYTHVSAAQLIDVYRKAHPHAK"
     gene            complement(6718..8127)
                     /gene="trmFO"
                     /locus_tag="PLT35_02700"
     CDS             complement(6718..8127)
                     /gene="trmFO"
                     /locus_tag="PLT35_02700"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOG28163.1"
                     /translation="MPAIRVIGGGLAGCEAAWQAASRGVSVTLHEMRPVRMTEVHRTG
                     DLAELVCSNSLRADKLDNAAGLLKAEMRRLGSLVMRAADATRVPAGAALAVDRRAFAA
                     YVTAAIDAHPLITVVRGEVTEIPAPAEAGGPVIVATGPLTSGRFAASLAAAVGEDCLY
                     FYDAISPIVSADSIDRRVAFRASRWDRSLGPEGAEAGGDYLNCPMNAEEYRAFHAALA
                     GAEGATLRDLDRAQFFEGCLPIEVLARRGADALRFGPMKPVGLADPRTGRRPYAVVQL
                     RQDSIAGDHYSLVGFQTQLKRSEQERVFRMIPGLAGAEFVRFGMVHRNTYINAPRALR
                     DTWQSRTRSDLFIAGQVSGVEGYVESAASGLLAGLNAGGAAAGGAAPTAAPPTTAIGA
                     LARYVSHASALAYQPSNITFGIIEPLESPPRHAHERRHALAARALADLDRWMIAAGLA
                     TQAAGGSPEASRSGPGSSR"
     gene            complement(8230..>10807)
                     /gene="topA"
                     /locus_tag="PLT35_02705"
     CDS             complement(8230..>10807)
                     /gene="topA"
                     /locus_tag="PLT35_02705"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOG28164.1"
                     /translation="VDVEGDFTPYYVIPAGKKKQVQALRAAVKQASELLLATDPDREG
                     ESISWHLREVLKPRIPVRRIVFHEITEGAVKEAVRGARDLDENLVRAQEARRILDRLY
                     GYTLSPVLWKKVGTGLSAGRVQSVAVRLLVEREEARRAFRSSRYWGLEAALTGGGRPF
                     TATLVRIGGQRLATSRDFDAGTGALAADGVRLLDEAQARELAGLLAGTLPWTVTSVEA
                     RPGLERPAPPFTTSTLSQEASRKLGFSTERTMQVAQRLFQGKEIGGGEIEGLITYHRT
                     DSTTLSEKAMGESARVIRELFGADYYAGPRRYQTRVKNAQEAHEAIRPTDFRLAPRDL
                     ERFLDADELRLYDLVWKRTVASQMADARVLKTSVEITAAGPAGEPCVFSASGKAIEFA
                     GFRRAYVEGSDDPDADLADQESILPPMREGDLVVGPGPSDAAPGGTGIALTRLEAKGH
                     ETQPPARFTEAALIRELEQAGIGRPSTYATTIATIERRGYVFRQGKALVPTFSAFAVT
                     NLLRDHFGDYVDVGFTAEMEEDLDEISNGERDAVSFIRAFYRDGDGGHAGLEPTVEKT
                     EPSIEYPAIEIGADPVSGEPLRVRVGRYGPFVQRGEGGPGNTATLPRQLPPADLTVEK
                     AIALLTTKAEGPKSLGVDPATGLRVYVHHGRFGHYVQLGEHPPRAAGKAAEKPKRASI
                     PGGAAASSITLDDALRLLSLPRELGPHPSDGEPVVAAVGRFGPYVKHGDEFRSLEDSD
                     DVYTVSLERALDLLKAPKKSRRRAAATTLRRQLGAHPQTGAEVRLMEGRYGPYVTDGH
                     VNASVPKTADASQVTLDDAVALLKARAEAGPAARKGAARAAGRAKRRAGKQTGAGSRH
                     PE"
BASE COUNT         1451 a         3984 c         3841 g         1531 t
ORIGIN      
        1 cggaccggca cttctcgagc cttctcaagg accaccacga catcgtgcgc gtcctcgcgt
       61 cggccttcga catggccacg ctgcccaacc gcgccgagct ccggctgagg ccgtcgggca
      121 aggtgattgg ctacacgctc tccctcgtgc gcgacgagcg gggccgcccc tacggagccg
      181 cgctgttctt caaggacctc acgctcgtgg agcaggcgga agagcgcgac cggctccggg
      241 accggctggc gacgctcggc gaaatggcgg ccatgatcgc ccacgaggtc aagaaccccc
      301 tcgccagcat cgaggtcatg gccggcctgc tgcggcgcca ggtgcccgat cgcgaggacc
      361 tccgcgagct ggccaccgac atcatcaacg aagccaagat ggccaatgcc atcgtcgtcg
      421 aggtgctgga gttcgtgcgg ccgatccggc tccaggtcga tcgcacctcg gtgccgaagg
      481 tgatcgaaga ggcggttccg ctggcggaat cgaaggtgcc gaggcgcgcg acgcggctcg
      541 agatcgacgt cccgggcggc ctgccggcga tccagggcga tcaccaccag ctcgtccagg
      601 tggtgacgaa cctgctcatc aacgcgctcg aggcgctcga cggcgggggg acgattcgcg
      661 tgggcgcgcg gctcgtcccg gaggatcacg acggctcggc ggccgtcgag ctcgtcatcg
      721 aagacgacgg acccggggtg ccgggcgaca tcacgagccg catcttcaat ccgttcttca
      781 ccaccaagcc gcagggatcg gggctcgggc tcccgatcgt gcggaagatc gtggacgccc
      841 acgacggccg gatcgacgtc gcgacttcgg ccggacgcgg caccacgttc accgtgacgc
      901 tgcccgtcgg agtgccgggg cagctgggtt tctaggggcg gcccaccgcc ccgcgcgccg
      961 gcaccggggt gccaggcgcg cgggcggcga gggcggcccc gcgaggtgcg catggctcgc
     1021 attctgattg ccgacgatca cgacgcgctc aggcgcgcgc tcgcccgggc cctggtggac
     1081 gccgggcacg aggtggagga ggccgccaac ggcaacctgg cgatcgagcg cctgcacgag
     1141 ggctccttcg acgtggtgct gagcgacctc aagatgggcg ggagcgacgg gatcgacgtg
     1201 ctgcgctcga cgaaggccct gcacccctcg gccgccgtca tcctcatgac ggcgttcggc
     1261 tcggttggca ccgcggtcga agcgatgaag atcggcgcct tcgactacgt ccagaagccg
     1321 ttcgagatcg aggagatgga gatcaagatc gggaaggcgc tcgagctgcg ccggatgcgc
     1381 cacgagatcg actacctgcg gcacacccag cacgacatct acaccttcga ctcgatcgtc
     1441 ggcgattccg aggcgctcag gaaggtgctg gccgtcgtcg gcaaggtggc gaggagcaac
     1501 accacgatcc tgatccacgg cgagaccggc acgggcaagg agcttgtcgc gggcgccatc
     1561 caccacaact cggcgcgatc gggccggaac ttcgtcaagg tcaactgcgc cgcgctccag
     1621 gagaatctcc tcgagtcgga gctcttcggc cacgagaagg gcgccttcac cggcgccgac
     1681 aagcagcgca tcggccgctt cgagcaggcg gacggcggca cgatgttcct cgacgaagtc
     1741 ggcgacatga gcccaagcac gcaggcgaag atcctcaggg tcctgcagga gcacgaattc
     1801 gagcgcctgg gcggcacccg gacgctgaag gtggacgtgc gcctcatcgc ggccgccaac
     1861 cgcagcctgc cggccatggt ggcggccggg cagttccgcg aggacctcta ctaccgcctc
     1921 aacgtcgtgt cggtggagat gccgccgctg cgcgagcgta aggaggacat cgccgcgctc
     1981 gcggacttct tcgtccgccg cttctccggg gagctcaaga agaagatcga cgggatcgcc
     2041 ccggacgccc tcaagctgct gatgcgctac aactggcccg gcaacatccg cgagctgcag
     2101 aacgccctcg agcgcgccgt cctgctcacc gacgggcacg tgatcaccgc ggacgatctc
     2161 aggctgggcg agacgacatc cttcggcagc gacgcggatg cggcgcgggt cgtgcgcgtg
     2221 ccgccggcgg gcatcgccct ccaggaaatc gagcggcagg cgctcgtcga gtcgctgaaa
     2281 atgtgcaact gggtccagaa ggacgcggcc gagctccttc gcgtcagtcc gcgggtgatc
     2341 aactacaaga tcaagacgct cgggatcaag ctgccgcgcg ggcgcggcgc cgcacccggg
     2401 cagaacgacg gctgagggcc gcgtcagtcg cgcggcggcc tgatcggcgc cgatggcggc
     2461 ggccgcgtgc ccggcacgac gccgggcgcg gtgagggaca tgccaggagc cggcgccggg
     2521 acgtacgtcg gcatcgcagg ggcgccgcgt tcctgcccga tgtcaatccg cgtcggctcg
     2581 tctccgtccg ccggctgccg ctgcgcgctg cgcgacggcc cctggaacgg cgggcgcatc
     2641 atgccgccgg gcggaggtcc gcccggcgcc gccaggtccg cgtcgtcgga cggctccccc
     2701 tgatcgtcgc tgatcccgag gaccacgcgg ccatccggaa ggacgcggcg ctgcatcggc
     2761 ccgcgcatgc cgacgcctcc tccgggcccc atgccgcctc cgccaaaggc ggaaccggag
     2821 ggcgcggctg gctggctggc cgccgaggcc cacgccgccg cctcgcccgg catgatgaca
     2881 atgcggctgt agcgggaaac gcccgccgcc gggtcgaggc gctcggccgc cgcgtaaccc
     2941 gccaccgcgc gcagcagcgt cgccagggcc cgcccctcgg cttcgtcaac gagctcgatg
     3001 gtcatgggcg tggcgggcgt gcgatcgcgg ttgaagatcc tcgtcccgcc gacgcgctcc
     3061 cattccgcca gaatctggcg cagcggcgcg tctcgggcgg acagcgtcac ccggccgtcg
     3121 cggatcgcga gcttcacctc gcccgcctgg agcagcccgg gcagcgcgag cccggccagc
     3181 agcgcaaggc cgcaccgaac cagcagtctc ctcgtcatgg ggcgctccct ctggtacgaa
     3241 gtatacgctc ccgcacgaaa ccggccaaga cggaccggcc cgcggcccgc agcggccctc
     3301 gacgaggctc agtcgacccg gatcttgacg acgaccttct gaaccggcgc cggcgcgccg
     3361 atcgccatgc ccggcatgtg cggctcgccg ggccagagca ggaggaagcg ccccggctgg
     3421 atgcgcagga agtcgccatc cccggccagg gcctcgaagt cccgctcggc gtcgtacgcg
     3481 ccccgcgtga aacgctcgat agcccccacc ccgaagcgtt cctccccgga aaccatgtac
     3541 tggaggtcgg cgtagacgcg gtgcgcctcc cacacgccgt cttgcggcag cttggacgtg
     3601 tagcgctgga tcatggcgta gaggctggcg ccgtcgatgt ccgcgcgccc ctcggcagcg
     3661 gccgcgaggt cggtccgggt aaggtaatcg aacgcgcggg cgaagcgcgg gccgagcgcc
     3721 aggtagcggt gcgcgttggc gaggcgatca gcgatcatcg tgcggccctc cgtggccggc
     3781 ctcgaacgat gccacaactg caggcccgag acaagccgcg cgggcccgca cacgcgcccc
     3841 cgtctcgcgg cccgccgcgc tgtcgccgtg gaggaaccgg ctggcgagct ggtgacggcg
     3901 acgccgatcg ccgccagcac gtccggggtc gccggcgccc gcgcttgcag gcgaggcgtc
     3961 ggcgctcgcc gtgcgggccg ccgcgctgga ggcggccggc gcctgctcga gctcttctgc
     4021 tccggcacac gggtccctcc cggcgggcgc acgctccccc gagagccggg tcccagaccg
     4081 tcgagcgctc ctccgcccgg ttccgaagta ccctgctggc atgaacccag cggacatcgc
     4141 gccggttgcg cccggggacc gccggcagtc gccgctcgac ttgttcgtca tcttcgccgg
     4201 cgcgaacatc gtcgccacga cgctgcaggt gggagcagcg ctggcggcgg acttcggcgc
     4261 catcgccgcc gtggcgctca tcgccgccgg cgcggtgggc ggctcggctc tggtggcctc
     4321 gctctccgtg ctgggcccgc ggctgggcgt gccgtccgtg attgccgcgc gcgcggccct
     4381 cggggtccgg ggcgcggcgc tcgtcgcggg gctcctctac gtcacgaact tcgcctggat
     4441 cgccgtcaac aacgtcatcg cggcgtccgt cggatcgcag atcctcggcg gccccggcgc
     4501 gatgccgatc tgggtcgtcg gcctcggcct cgtgtccact gccgtcgtgg ccctcgggcc
     4561 tcgcgccgtc ggcctcgccg atcgcgtcgc ggtgccgctg ctgctgctgg tcggcctcat
     4621 catcacctgg gccgtcgtcc aggcgccgcc ggcgccggtg gcgatgcgtc agccgcccgc
     4681 cgcgcccgcc cactggctcc gcggcttcga catcgtcgtc ggctaccagg tgtcgtggat
     4741 cctgatgttc gccgacttct cgcgctacac gacgtcggag cgcgcaggcg ccgcggccgt
     4801 gttcttcggg ttgctgctca cgagcctctg gatgatgccc ctcggcctcg cggcggcgcg
     4861 cgtggcccac agcgcggacc cgggcacgat gatggcggct gtcggcctgg gctgggccgg
     4921 cgccgccctc atggtgctgg cgacgatcac cacgaacttc gtcaacatct acatgtcggg
     4981 cctggcgtgg aagagcctgt tcccgcgcgc gggcgcccag tcggccgtct ggtccgtcgg
     5041 gatcgtcggc acgctcttcg gcctgtgcta cggaacctgg ctggatcgct acgccgaata
     5101 catggtggtt ctgggcggca tcctggtccc cgtcggcggc gtgctcgtcg cgcatttccg
     5161 gctgagccgg acgaccacgc acgtgccgga cctctacgac ccggccgggc cgtgccgggg
     5221 ggtgctcctg cccggcgtca tcgcctgggc gctcggcgcc ttcgcctact acgtcgccag
     5281 ctcctggggc ggcaccctgc ccgcgctcgc gacggcagtc gtctcctatc tgctgctgcg
     5341 ccgaaggctc gcatggcgct gacgccccgg catctggcgc tggccaccgc ccgggccggc
     5401 gtcgtgtcgg acccgcagcc gacactggac cacttcgcgg ccccgggacc caggctgccg
     5461 tttgtcgcgg gcctggcgga ggacgccgga ggccgggcgt ggccgcggcg cggcgggaca
     5521 gccagctccc ggacgcaagc ggcgccaccg tcgcgcaagc atggacgtgc ggcgcgcgca
     5581 ccggggtgcc gcacggcgcc gagtccgcgg gctggcgttt tcccgaggcc gggctcacag
     5641 ggcggtccgg cccggtcgtg ccgcgccgcg ggcgcctgcg gtggcggatg cgcgaagagc
     5701 cagcggcagg gtgggcgctc gcaacgcccc tgcggggccg cgcctacttc gcgtgcgggt
     5761 gggccttgcg gtagacgtcg atgagctgcg ccgcgctcac atgggtgtac cgctgcgtcg
     5821 tggacagctg ggcgtggccg agcagttcct ggatggatct gaggtcggcg ccgcgctcga
     5881 gcaggtgcgt cgcgaacgag tggcgcagcg cgtgcgggct gacgccgagc ctcgcgctgc
     5941 acgccgccac gtagcgccgg acgagccggt gcacgctgcg gccggtcagc cgcccgcctc
     6001 ggaagttcag gaacagcggg ccggaacccg ccgtgcgcct cggggcgggg cggccggcgc
     6061 cgctccttgg cgccggcctg gccgggccgg accgctggcg ggctgctgcc cgttccagat
     6121 cgggacggtc cctcaggtac gcccgaatcg cctgctcggc gctggtgttg aacgggacga
     6181 cccgctcctt gccgcccttt cccaacaccc ggaccatccg cccgccgaga ttgacgtgat
     6241 ccagatcgag gccgacgagc tcgccgagcc gcagcccgga tgcgtagagc agctccagaa
     6301 tcgcccggtc gcgccggccg agggccgtgc cctcgttcgg cgtctcgagc agccgggtca
     6361 tctcgtcgat ctcgaggtgc gcggggagct tcgcgtcgag gcgcggagac gagacgaggg
     6421 agccgggatc gccgtcgatg agcgactcgc ggcggaggta gcggacgaag gcgcggaccg
     6481 aggccagctt gcgcgccgcg gtcttgcgcg atcggcgcgt ccggtacagc tcggcgagga
     6541 acgagcggat ggcggcgaca tcgcagtcgg acggccgctg gtcccgcgcc gccttgccgt
     6601 tgtggatcgg aaggaagccg atgaactggg agagatcgcc ctcgtacgcc cggaccgtgt
     6661 gcggcgaggc gccgcggttg tcgcgcagat gcacgaggaa cgccttcaag tggcccttca
     6721 tcgcgacgag cccggcccgg accgtgaggc ctcggggctc cctcctgcgg cctgcgtcgc
     6781 caggcccgcc gcgatcatcc accgatcgag atcggccagc gccctggccg cgagcgcgtg
     6841 ccgccgctcg tgcgcgtgcc gcggcgggga ctcgagcggc tcgatgatcc cgaaggtgat
     6901 gttgctgggc tgataggcga gcgccgaggc gtgcgacacg tagcgcgcca gcgccccgat
     6961 ggccgtcgtc ggcggcgcgg ctgtcggcgc cgcgccgccg gcagccgcgc cgccggcatt
     7021 gaggccggcc aggagcccgg acgccgcgga ttcgacgtag ccttcgacgc ccgatacctg
     7081 gcccgcaatg aaaaggtcgc tccgggtccg ggactgccac gtgtcgcgga gcgctcgcgg
     7141 ggcgttgatg taggtgttgc gatgcaccat gccgaagcgc acgaactccg cgcccgccag
     7201 cccgggaatc atccggaaca cgcgctcctg ctcgctccgc ttcagctgcg tctggaaccc
     7261 gacgaggctg tagtggtcgc ccgcaatcga gtcctgcctg agctggacga cggcgtaagg
     7321 ccggcggccc gtgcgaggat cggcgagtcc caccggcttc atcggcccga accgcagcgc
     7381 gtcggcgccc cggcgggcca ggacttcgat tggaaggcag ccctcgaaga actgcgcgcg
     7441 gtcaaggtcg cggagcgtcg cgccctcggc gcccgcgagc gccgcatgga aggcccggta
     7501 ttcctccgcg ttcattgggc agttgaggta gtcgcctccg gcctccgcgc cctccgggcc
     7561 gaggctccga tcccaccgcg aggccctgaa tgccacgcgc ctgtcgatgc tgtccgccga
     7621 gacgatgggg ctgatggcgt catagaagta caggcagtcc tcgccgacgg cggcggcaag
     7681 cgacgcggcg aaccggccgc tcgtgagcgg ccccgtggcg acgatcaccg gcccgcccgc
     7741 ttccgccggc gcggggatct ccgtcacctc cccgcgcacg acggtgatga gcggatgagc
     7801 gtcgatcgcc gccgtgacgt acgccgcgaa cgctcgccga tccacggcga gcgccgcgcc
     7861 ggccggaacc cgcgtcgcat ccgcggcgcg catgacgagc gagcccaggc gccgcatctc
     7921 ggccttcagc agtccggcgg cgttgtccag cttgtcggcg cggagcgaat tgctgcagac
     7981 cagctcggcg aggtcgcccg tccggtgcac ctcggtcatc cggacggggc gcatctcgtg
     8041 cagcgtcacc gagacgccgc gggacgccgc ctgccacgcg gcctcgcatc ccgccagccc
     8101 cccgccgatg acgcgaatcg caggcatggt gccgcccatc gccgccttgc tcgcccgtcg
     8161 tacgccgaac ccagggcctc gcgcgcagac gaccgtctgc tgtggcgggc cctccgcagc
     8221 cggcaccccc tactcgggat gccgactccc ggctcctgtc tgcttccccg cccgtcgctt
     8281 cgcgcggccc gcggcgcgag cggcgccctt gcgcgccgcc ggccccgcct cggcccgcgc
     8341 cttcagcaag gcgacggcgt cgtcgagggt cacctgcgag gcgtccgcgg tcttcggcac
     8401 cgacgcgttg acgtggccgt ccgtcacgta ggggccgtag cggccctcca tgagcctcac
     8461 ctcggcgccc gtctgcgggt gggcgcccag ctggcgccgg agcgtcgtcg cggcggcgcg
     8521 gcgccggctc ttcttcggag ccttcagcag atccagcgcc cgctccagcg acacggtgta
     8581 gacgtcgtcg ctgtcctcga gcgacctgaa ctcgtcgccg tgcttcacgt acgggccgaa
     8641 gcggccgacg gccgcgacca ccggctcgcc gtccgacggg tgcgggccaa gctcgcgagg
     8701 caggctgagc agccgcagcg cgtcgtccag cgtgatcgag gacgcggccg cgccgcccgg
     8761 gatcgacgcc cgcttcggct tctcggcggc cttgcccgcc gcccgcggcg ggtgctcccc
     8821 aagctgcacg tagtgcccga accgcccgtg atgcacatag accctcagcc ccgtcgccgg
     8881 atccacgccg aggctcttcg gcccttcggc cttcgtcgtg agcagcgcga tggccttctc
     8941 caccgtgagg tccgccggcg gcagctggcg cggcagcgtg gccgtgttgc ccggcccgcc
     9001 ctcgccgcgc tggacgaacg gcccgtaccg gccgacgcgc acgcgaaggg gctcgccgga
     9061 caccgggtcg gcgccgatct cgatggccgg gtactcgatg ctcggctccg tcttttccac
     9121 cgtcggctcg agcccggcgt gtccgccgtc gccgtcccgg tagaacgccc ggatgaagga
     9181 cacggcgtcc cgctcgccgt tcgagatctc gtcgaggtcc tcctccatct ccgcggtgaa
     9241 gcccacgtcc acgtagtcgc cgaaatgatc gcgcagcagg ttcgtcaccg cgaaggctga
     9301 gaaggtcggc acgagggcct tgccctgccg gaacacgtag ccgcgccgct cgatggtggc
     9361 gatggtcgtg gcataagtcg agggccggcc gatgccggcc tgctccagct cccggatcag
     9421 cgcggcctcg gtgaagcgcg ctggaggctg cgtctcgtgc cccttggcct caaggcgcgt
     9481 cagcgcgatc cccgtgccgc ccggcgccgc gtccgacggt cctggcccga cgacgaggtc
     9541 tccctcgcgc atcggaggca ggatcgactc ctggtccgcc aggtccgcat ccggatcgtc
     9601 gctgccctcg acgtacgccc gccggaagcc ggcgaactcg atcgccttcc cgctcgccga
     9661 gaagacgcac ggctcgccgg cggggcctgc ggcggtgatc tcgacgctgg tcttgagtac
     9721 ccgcgcgtcg gccatctgcg acgccacggt gcgcttccag acgagatcgt acaagcgcag
     9781 ttcatcggca tcgagaaacc gctcgaggtc ccgcggcgcg aggcggaaat cggtcggtcg
     9841 gatggcttcg tgggcttcct gggcgttctt gacccgcgtc tgatagcggc gggggcccgc
     9901 atagtagtca gctccgaaga gctcccggat gacgcgcgcc gactcgccca tcgccttctc
     9961 gctgagcgtc gtcgagtcgg tccggtggta cgtgatgagg ccctcgatct cgccgccgcc
    10021 gatttccttg ccctggaaca ggcgctgcgc cacctgcatc gtgcgttcgg tcgagaaccc
    10081 gagcttccgc gaggcttcct gcgacagcgt ggacgtcgtg aacggcgggg ccgggcgctc
    10141 gaggcccggc ctggcctcga ccgacgtgac cgtccacggc agcgtccccg ccagcaagcc
    10201 agccagctcg cgcgcctgcg cctcgtcgag cagccggacg ccgtcggcgg cgagcgcacc
    10261 ggtgcccgcg tcgaagtccc tgctcgtcgc caggcgctgg ccgccgatgc gcacgagggt
    10321 cgccgtgaag ggccggccgc cgcccgtgag cgcagcttcg aggccccagt agcggctgga
    10381 tcggaacgcg cgccgcgctt cctcgcgctc gaccagcagc cggacggcca cgctctgcac
    10441 gcgccccgcg ctcaagcccg tgccgacctt cttccacaac accggcgaga gcgtgtagcc
    10501 gtagagccgg tcgaggatcc gccgcgcctc ctgggcgcgg acgaggttct cgtccagatc
    10561 gcgcgccccc cgcacggcct ccttgacggc gccctcggtg atctcgtgga acacgatgcg
    10621 ccgcaccgga atcctcggct tgagcacctc gcgcaggtgc cagctgatcg attcgccttc
    10681 gcggtcgggg tccgtcgcga gcaggagttc ggacgcctgt ttgacggccg ccctgagcgc
    10741 ctgcacctgc ttcttcttgc cggcgggaat gacgtagtag ggcgtgaaat cgccctccac
    10801 gtccacc
//
LOCUS       DAOSIF010000042        10712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6054_length_10712_cov_3.912264, whole genome shotgun sequence.
ACCESSION   DAOSIF010000042 DAOSIF010000000
VERSION     DAOSIF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6054_length_10712_cov_3.912264"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PLT35_02710"
     CDS             complement(<1..1050)
                     /locus_tag="PLT35_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28165.1"
                     /translation="MIEPLDYEAVGFMSGLEVHQQLLTAHKLFCRCPGGLYTREHDGE
                     VLRHMRPTLSELGEYDGTALMEFKTKKEIIYLLNHLNVCTYEMDDTPPFLVNQEAIDI
                     AIELCLMMHMDIIDEVHIARKQYLDGSIPTGFQRTAIVGVNGWLPFKGRRLAVTHVSV
                     EEDSCREVSDKGHRIVWRTDRLGMPLTETVTAPDLRTPGEVRDAIVLCGLTARSTRRV
                     RTGIGASRQDVNVSVRGGSRVEIKGVPRAPLAARLVHNEAVRQCNLLRLRSELRRRGL
                     SAPDAIRVTAADVSDIWSGADLGFIRRALQSGGRVHAVRVSGAAGLAQHPTQPDTTFL
                     DELSGRIRVIACLDEV"
     gene            complement(1184..1963)
                     /locus_tag="PLT35_02715"
     CDS             complement(1184..1963)
                     /locus_tag="PLT35_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG28166.1"
                     /translation="MNLELTDKVAVITGSSRGLGFASARALADEGCRVVICGRTAGRL
                     DEAAAELRASCGSPARIAAIQADVSTEAGVRAVIDGAAARFGGLDILVNNVGLARGGD
                     LLATTDAEWQEAVDQTLMPAVRASRLAVPHLRARGGGVILMIASIFGRETGGRMTYNA
                     VKAAEISLAKSLAQQLAGDNIRVNSIAPGSILFPGGSWWKRQQADPEGIAEFVRRELP
                     FGRFGRPEEVGAVVAFLASPRASWVSGASIVVDGCQSRSMI"
     gene            complement(2000..2851)
                     /locus_tag="PLT35_02720"
     CDS             complement(2000..2851)
                     /locus_tag="PLT35_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG28167.1"
                     /translation="MSSPPDRRCCSAGGRALSFLRAGDPDPARLVVFLHAFPLSAEMW
                     RPQLAALPPGWAGAAPDYRGFGLSDPDGEAPAGRARARIEDYAADIGVLLDAIGAERA
                     ALCGCSLGGYVALAALRLFPRRVAGLLLAATRAGADSEAARSGRLAALERLERDGPAA
                     VGADMRAKLIGSSSRAHRPDVVAGVERMMEGLTATGVGYAVTRMMFRPDSSADLAAFR
                     GPVTVVVGEEDVLTPPADGAALAALAPGAALETIPGAGHLPNLEAPESFNGAMLAWLQ
                     AIERSSA"
     gene            complement(2931..5729)
                     /locus_tag="PLT35_02725"
     CDS             complement(2931..5729)
                     /locus_tag="PLT35_02725"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOG28168.1"
                     /translation="MERGSEQVGPVGTPIAGRGVDAADQWSAQAPGLEYTRVFSQAGR
                     DPYDGIEWDRRDAVIANERGEAVFEQRGVEVPRSWSQQATNIVASKYFRGLLGSPDRE
                     WSVRQLIDRVVRTIVAWARAQRYFASEADLEAFGDDLTHLLAHQQGAFNSPVWFNCGI
                     EPHPQLSACFINSVEDTLESILTLAKTEGMLFKFGSGTGTNLSSLRSSREHLAGGGTA
                     SGPVSFMKGFDAFAGVIKSGGKTRRAAKMVILNADHPDIVEFITCKVDEEKKAWALID
                     AGYDGSFTGTAYGSVFFQNSNNSVRATDDFMRAVLDDGPWQTRAVKDPSRVMDTYRAR
                     DLMRQIAEATHVCGDPGMQFDTTINEWHTCPATERINASNPCSEYMFLNDSACNLASI
                     NLMKFVDARGEFDVDAFKSAVRAFITAQEIIVGYASYPTKAIERNSHDYRPLGLGYTN
                     LGALLMSRGLPYDGDAGRAYAAAITALMTGEAYAQSARIARDHGGPFAGYAKNREPFL
                     RVMRKHRDALRDLNTAMVPASLREAATQAWDEAIEIGEEHGYRNAQATVLAPTGTISF
                     MMDCDTTGIEPDIALIKYKKLVGGGLMKLINTTVPSALARLGYTAAQTKAIVDYTDAH
                     ETIEGAPHLQEKDLPVFDCAFKPAKGSRSIHYTGHIKMMGAVQPFISGAISKTVNVPK
                     DATPEDIMQAYIEAWRLGVKAIAIYRDGSKRMQPLNTSKDAKAKPASAGAAPAPALPV
                     RRKLPEERQAITHKFDIQGHEGYLTVGLYEDGMPGELFLVMAKEGSTISGFADAFAQA
                     ISYALQYGVPLHVLVDKFSHMRFEPSGMTKNPQVRFAKSIVDYVFRWLAAKFLSSEAQ
                     YRVGVNMAGAQAREGAADAEQLRLEVAPASEVAVGAEMMTVQNQEDAPPCSLCGAIMV
                     RSGSCYKCINCGNTSGCA"
     gene            6336..6998
                     /locus_tag="PLT35_02730"
     CDS             6336..6998
                     /locus_tag="PLT35_02730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG28169.1"
                     /translation="MLASGFPTQLALGALLASAGLSPVGSDGRLSMQYLALLMPADTL
                     LLSAFIVWRLRAGGGRASTVLLGSRGWAREAWLGILLVPLVVGGVAGFLLALRTAWPG
                     LHNVDVNPFEALLRTPGDVAVLVVLVTLAGGVKEELQRAFVLHRFSQSLGGARAGLLL
                     YSVIFGAGHVIQGYDVALATTLLGVAWGAVFLWRRSVVAASVSHAGFNAAQVLHFAVF
                     GA"
     gene            7051..8424
                     /locus_tag="PLT35_02735"
     CDS             7051..8424
                     /locus_tag="PLT35_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28170.1"
                     /translation="MTRRLYASDEVQYVSFLRSLWFDGDLSFDNEYRHFHEAGPARDA
                     GFVETFLIPATETGRRRTFGTIGSALLWAPFYAVADAGTRAARALGGDVQADGYSAPY
                     ISAICVGSAAYGILALLLAWFAARRVLALAGEARAAPATAATVAVGLGTPLLFYMYVA
                     PGYAHATSAFAASAFVLAWLAVREEWSLRGLAGLGALAALMAMVREQDAFIVVGPAID
                     LLWTVVGERRWRLIVRALAGAAVAAAAYLPQAAAYLLINGHLGPSRLVSRKMTWTAPH
                     ALEVVCSTEHGLIWWTPLALAAMAGIGLLAAGAGAAPGAAADCGNRRSAARIGAGLAA
                     IVLLQIYIAGSVESWTVAGAFGQRRFVALTAPFVIGLAVVGARLASVRARTAAAALAA
                     VCVWWNLGLMVQFGAGLMDRQRLELRRNAYNTFIEVPQRLPELAHRYLFDRASFYRPA
                     APGRGPG"
     gene            8441..9634
                     /locus_tag="PLT35_02740"
     CDS             8441..9634
                     /locus_tag="PLT35_02740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28171.1"
                     /translation="MLIRYFADIRFPLERANGVQTMETCHALARRGHLVHLVVRRDSA
                     DPPRDPFAYYGLPSHPLLSVERLRVPAGEAPRRAMFIAKSLRRVTGIPRADAILTRDL
                     AFAALLVRLPRPWRSPVVYESHGYAPAVAEDLPAMVSGAAPLSTAKRRRLEARERLVW
                     AAADGYVTITAALARELEDRFGARTRLAVIPDGARLAPIDGTGLRARADGEAPVVAYA
                     GHLYPWKGADVLVEAVARLPEVRAVVVGGMPGEPDLPRVCALADRIAPGRVRFTGLVE
                     PPRVPALLRDADVLVVPNTPGRASASYTSPLKLFEYMASGRPIVASDLPALGEVLRRD
                     ENAVLVEAGNAEALAAALARVLGDAPLAARLAAQAREDASGWTWDARARRLEALLDAV
                     AGGAA"
     gene            9631..10695
                     /locus_tag="PLT35_02745"
     CDS             9631..10695
                     /locus_tag="PLT35_02745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG28172.1"
                     /translation="MISDRLLQLVRCPDCGGRLGGRPPGLVCESCSRPFATDPDFLVL
                     HPGITFAEQTKYLDAALHADARHERVTPPLLGARIRNDVLRSFLAPGPGDAVIDLGCG
                     SGRTLVWNAGLGAYQAGIDVSPYFAREARAQADLVLGDLRRLPFAAGAFTKASALDVF
                     EHLSRDALAGVLAEIARVLAPGGALFVYSHVRKNSRLAGGLRQINRLARWMERRGLVD
                     LSRERLRKSDHLNPLADIPDLESAVAGAGFRIERIRYYTPLVGGFVENILVRAGEHWL
                     AKRAAARVAAGGGGAGRDAAVRDVRAAVKRRAGESGPAYWGLRAVTWIMKIDLVLFGR
                     VRSGPYFALLRHLPAEGGAP"
BASE COUNT         1369 a         3810 c         3794 g         1739 t
ORIGIN      
        1 cacctcgtcg aggcaggcga tgacgcggat gcggcccgac aactcgtcga ggaaggtcgt
       61 gtcgggctgc gtcgggtgct gcgccagccc cgccgcgcct gacacccgca ccgcgtgaac
      121 gcgcccgccc gactgcaggg cccgccggat gaagcccagg tccgctcccg accagatgtc
      181 ggacacgtcg gccgccgtga cgcgaatcgc gtccggggcc gacaggccgc gccggcgaag
      241 ctcgctgcgg agcctgagca gattgcactg ccggacggcc tcgttgtgca cgagcctggc
      301 ggccagtggc gcgcgcggca cccccttgat ctcaacgcga gatcctccgc ggaccgacac
      361 gttcacgtcc tggcggctgg cgccgatgcc cgtccgcacg cgccgcgtcg accgcgccgt
      421 cagcccgcag agcacaatgg cgtcgcggac ctctccgggc gtccgcaagt cgggcgcggt
      481 cacggtttcg gtgagcggca tgcccagccg gtcggtccgc cagacgatgc gatgcccctt
      541 gtcagacacc tcccggcagg agtcctcctc cacgctgacg tgggtgaccg ccaggcggcg
      601 gcccttgaac ggcagccagc cgttcacccc gacgatggcg gtccgctgga acccggtggg
      661 aatcgacccg tcgaggtact gcttccgtgc gatgtgcacc tcgtcgatga tgtccatgtg
      721 catcatcagg cagagctcga tggcgatgtc gatggcctcc tggttcacga ggaacggcgg
      781 cgtgtcgtcc atctcgtagg tgcagacgtt cagatggttc aggagataga tgatctcctt
      841 cttcgtcttg aactccatga gcgcggtgcc gtcgtactcg cccagctcag agagcgtcgg
      901 gcgcatgtgc cgcagcacct cgccgtcgtg ctcgcgcgtg tagagcccgc cggggcagcg
      961 gcagaagagc ttgtgggcgg tgagcagctg ctggtgcact tcgaggcccg acatgaagcc
     1021 caccgcctcg tagtcgagtg gctcgatcat cggcatcctt ccgcgccgcg cacgtcgtgc
     1081 gtcgtccggg gggaggcagg catctgtctt ctggatcttg gattcctgat tctgtcttct
     1141 gaattccggg ttctggattc ctgcttcctg cttcctgctt ccttcagatc atcgatctcg
     1201 actggcagcc gtcgacgacg atgctcgcgc cgctcaccca gctggcccga ggcgacgcca
     1261 ggaacgcgac cacggccccg acttcctccg gccggccgaa ccgtccgaac ggcagttccc
     1321 ggcgcacgaa ctcggcgatg ccttcgggat ccgcctgctg gcgcttccac cacgacccgc
     1381 ccgggaagag gatcgagcct ggtgcgatgc tgttcacgcg gatgttgtcg cccgcgagct
     1441 gctgcgcgag cgacttggcc aggctgatct cggcggcctt caccgcgttg taggtcatgc
     1501 ggccgccggt ttcgcggccg aagatcgagg cgatcatgag gatcacgccg ccgcccctgg
     1561 cccgcaggtg cggcacggcg agccgcgacg cgcgaaccgc aggcatcagc gtctggtcca
     1621 ccgcctcctg ccactcggcg tccgtcgtgg ccagcaggtc gccgccgcgc gcgaggccga
     1681 cgttgttcac caggatgtcg aggccgccga agcgtgccgc cgcgccgtcg atgacggctc
     1741 ggacgccggc ctcggtggac acgtcggcct ggatggcggc gatgcgcgcg gggctgccgc
     1801 acgaagcgcg cagctccgcg gccgcctcgt ccagccggcc tgcggtccgc ccgcagatga
     1861 ccacgcggca cccttcatcg gcgagggcgc gggcgctggc gaagccgagg ccgcggctcg
     1921 atcccgtgat gacggcgacc ttgtcggtga gttcgaggtt catggtcggg gttcgggttt
     1981 cggggtcagg cgcgcagcct cacgcgctcg aacgctcaat ggcctgcagc caggcgagca
     2041 tcgcgccgtt gaacgattcc ggggcctcca ggttcggaag atgccccgcc ccggggatcg
     2101 tctcgagcgc agcgcctgga gccagggccg ccagcgccgc gccgtcggca gggggcgtca
     2161 ggacgtcctc ctcgccgacc acgacggtca ccgggccgcg aaacgcggca aggtcggccg
     2221 acgaatccgg ccggaacatc atccttgtca cggcgtagcc gacgccggtt gccgtcaggc
     2281 cttccatcat gcgctcgacg cccgcgacca cgtcgggcct gtgggcgcgg gagctgctgc
     2341 cgatcagctt cgcccgcatg tccgcgccca cggccgccgg gccgtcgcgc tcgaggcgct
     2401 ccagggccgc gagccgtccg gatcgggcgg cctcgctgtc ggcccccgcg cgcgtggccg
     2461 ccagcagcag gccggcaacg cgccggggaa acagccggag ggccgccagc gccacgtacc
     2521 ccccgagcga acagccgcac agcgcggctc gctctgcgcc aatcgcgtcc agcaggacgc
     2581 cgatgtccgc cgcgtagtcc tcgatgcgcg cgcgagcgcg gccggccggc gcttcgccgt
     2641 ccgggtcgga cagcccgaac cccctgtagt ccggcgcggc gcctgcccac ccgggcggaa
     2701 gggccgccag ctgcggccgc cacatctcgg cgctcagcgg aaacgcgtgc aggaagacga
     2761 ccaggcgcgc tgggtccggg tctccggcgc gcaggaagct cagggctcgg ccgcccgcgg
     2821 agcagcagcg gcggtcgggt ggggaggaca tgaggcgatc atacccgggg cgcggggggc
     2881 ggggctctgg attccggcgc tctcgcctcc tgttcctccc gccccttccc ctacgcgcac
     2941 ccgctcgtgt tgccgcagtt gatgcacttg tagcagcttc cgctgcgaac catgatcgcg
     3001 ccgcacaggc tgcacggcgg cgcgtcctcc tggttctgca cggtcatcat ctcggccccg
     3061 acggccactt cgctcgcggg tgcgacctcg agcctgagct gctcggcgtc ggcggctccc
     3121 tcccgggcct gcgcgccggc catgttgacg cccacgcggt actgggcctc ggacgacagg
     3181 aacttcgccg cgagccagcg gaagacgtag tccacgatgg acttggcgaa ccgcacctgc
     3241 gggttcttcg tcatgcccga gggctcgaac cgcatgtggc tgaacttgtc cacgagcacg
     3301 tgcagcggca cgccgtactg gagcgcgtag gagatggcct gcgcgaaggc gtcggcgaag
     3361 ccggagatgg tcgatccctc cttggccatc acgaggaaca gttcgccggg catcccgtct
     3421 tcgtaaaggc cgacggtgag gtagccctcg tggccctgaa tgtcgaactt gtgggtgatg
     3481 gcctggcgct cctcgggcag cttgcggcgc accggcaggg caggcgcggg cgccgctccg
     3541 gcggacgcgg gcttggcctt ggcgtccttc gacgtgttca gcggctgcat gcgcttgctg
     3601 ccgtcgcggt agatggcgat cgccttcacg cccaggcgcc aggcctcgat gtaggcctgc
     3661 atgatgtcct cgggcgtggc gtccttgggc acgttgacgg ttttcgagat cgcccctgag
     3721 atgaagggct gcacggcgcc catcatcttg atgtggcccg tgtagtggat ggatcggctg
     3781 cccttggcgg gcttgaaggc gcagtcgaag accggcaggt ccttctcctg gaggtgcggc
     3841 gccccctcga tggtctcgtg cgcgtcggtg tagtcgacga tggccttcgt ctgggcggcg
     3901 gtgtagccga gcctcgcgag ggcggagggc acggtcgtgt tgatcagctt catcagcccg
     3961 ccgcccacca gcttcttgta cttgatgagg gcgatgtcgg gctcgatccc ggtggtgtcg
     4021 cagtccatca tgaagctgat ggtgccggtc ggcgcgagca cggtcgcctg ggcgttgcgg
     4081 tagccgtgct cctcgccgat ctcgatggcc tcgtcccacg cctgggtggc ggcctcgcgg
     4141 agcgaggcgg gcaccatggc cgtgttgagg tcgcggagcg cgtcgcggtg cttgcgcatg
     4201 acgcgcagga acggctcgcg gttcttcgcg taccctgcga agggcccgcc gtgatcgcgg
     4261 gcgatgcggg ccgactgcgc gtaggcctcg cccgtcatga gggcggtgat ggcggcggcg
     4321 taggcccggc cggcgtcgcc gtcgtagggc agcccccgcg acatcagcag cgccccgagg
     4381 ttcgtgtagc cgaggccgag cggccggtag tcgtggctgt tccgctcgat ggccttcgtc
     4441 gggtagctgg cgtagccgac gatgatctcc tgcgcggtga tgaacgcgcg caccgcgctc
     4501 ttgaacgcgt caacgtcgaa ctcgccgcgc gcgtccacga acttcatcag gttgatcgac
     4561 gcgaggttgc acgccgagtc gttcaggaac atgtactccg agcacggatt cgacgcgttg
     4621 atgcgctccg tcgccgggca cgtgtgccat tcgttgatcg tcgtgtcgaa ctgcatcccc
     4681 ggatcgccgc agacatgcgt cgcctcggcg atctggcgca tgagatcccg cgcccggtac
     4741 gtgtccatga cccgcgacgg gtccttcacc gcccgcgtct gccacggtcc gtcgtcgagg
     4801 acggcgcgca tgaagtcgtc ggtcgcgcgc acgctgttgt tcgagttctg gaagaacacc
     4861 gacccgtacg ccgtgcccgt gaacgaaccg tcgtacccgg cgtcgatgag agcccaggct
     4921 ttcttctcct cgtccacctt gcaggtgatg aactcgacga tgtccgggtg atcggcattc
     4981 aggatcacca tcttcgccgc gcgccgggtc ttgccgcccg acttgatgac cccggcgaac
     5041 gcgtcgaagc ccttcatgaa cgagaccggc cccgacgccg tcccgccccc cgccaggtgc
     5101 tcgcgcgacg agcgcaggct cgacaggttc gtcccggtcc ccgagccgaa cttgaacagc
     5161 atcccctcgg tcttggccag cgtcaggatc gactccagcg tgtcctcgac cgagttgatg
     5221 aagcacgccg acagctgcgg gtgcggctcg attccgcagt tgaaccacac ggggctgttg
     5281 aacgcgccct gctggtgcgc cagcaggtgc gtcaggtcgt cgccgaacgc ctccaggtcg
     5341 gcctcgctcg cgaagtagcg ctgcgcccgc gcccacgcga cgatcgtgcg caccacccgg
     5401 tcgatgagct gccggacgct ccactcgcga tcgggcgagc cgagcaggcc gcggaagtac
     5461 ttcgaggcga cgatgttcgt cgcctgctgc gaccagctgc gcggcacctc gacgccgcgc
     5521 tgctcgaaca cggcctcgcc ccgctcgttc gcgattaccg cgtcgcggcg gtcccactcg
     5581 atcccgtcat acgggtcccg cccggcctgg gagaacaccc gcgtgtactc caggcccggt
     5641 gcctgcgcgg accactggtc cgcggcgtcg acaccccgac ccgcgatggg cgtacccacc
     5701 ggacccacct gctccgatcc gcgttccatg ccgcctccgc cgccgcagcg cgtccgggcc
     5761 cctcaggcgc gccgagccga ccgataaccg tgtgcgaaac gacgagtccc ccgcccgagc
     5821 cgcagaacgc gatcccgcgt tccagagaca gcgcagccgc cagccagccg gcccatgacc
     5881 cgcattgcca ggttcgctcg tggcgctggg ctccggccga cctcgtgacg aatcgtggaa
     5941 actcgagctg cgaagcttgg cgagaagctg ggaggttctc gtcgtagcta atccgtaacg
     6001 agggcataat atgcgccgat cacccccggc gtgtcaagtg caaacctaca agatattgtg
     6061 ctttcggatt aggtcatacg caatagagtg tgtattccgg cctattcccg cgacttttgc
     6121 gttatcttcg cttcgcggcg ccgcgcaggg cgcccgccgc gtcgaacccg atccgcaggg
     6181 cgggcttccg ggccgttcga cgcctcgcgt gatagagtca cgggaacgtg cagcacgtcc
     6241 caacagaggc gcccgaggcg cctccggccc cgcctgctcc ggcccgccaa gttccgcggg
     6301 gcgccccatc gccgcttgcc gcagccgccg acgtcgtgct ggcttcgggc ttcccgaccc
     6361 agctggcgct tggcgctctg ctcgcgtccg ccggcctctc gcccgtcggg tccgacggcc
     6421 ggctgtcgat gcagtacctc gccttgctga tgcctgcgga cacgctgctg ctgtcggcct
     6481 tcatcgtctg gcgcctgcgc gccggcggcg gccgggcttc gacggtgctg ctggggtcga
     6541 ggggctgggc gcgggaggcc tggctcggca ttctgctcgt gccgctcgtg gtgggcggcg
     6601 tcgcaggttt cctgctggcg ctgcgcacgg cgtggccggg gttgcacaac gtcgacgtca
     6661 atcccttcga agcgctcctc cgcacgcctg gggacgtcgc cgttctcgtc gtcttggtca
     6721 ccctcgcggg cggcgtcaaa gaagagctgc agcgcgcgtt cgtgctgcac cgcttcagcc
     6781 agtcccttgg cggcgccaga gcgggcctgc tgctctatag tgtgatcttc ggcgccgggc
     6841 acgtcatcca gggatacgac gtcgcgctgg cgacgacgct gctcggggtg gcgtggggcg
     6901 ccgtctttct ctggcgccgc agcgtcgttg cggcgtcagt cagccatgcg gggttcaatg
     6961 cggcgcaggt tctgcacttc gcggtgttcg gcgcctgaag gccgggcgat cctggccgcc
     7021 gggttgatct tcgttctcac actccccggc gtgacccggc gcctctacgc gtccgacgag
     7081 gtgcagtacg tctcgttcct gcgctcgctg tggttcgacg gtgatctgtc cttcgacaac
     7141 gagtaccgcc acttccatga ggccggaccg gcccgtgacg cgggcttcgt cgagacgttc
     7201 ctgatccccg cgacggaaac cgggcggcgg cgcacgttcg gcacgatcgg ctcggcgctc
     7261 ctgtgggcgc cgttctacgc ggtcgccgac gcgggcacgc gcgcggcgcg ggcgctcggc
     7321 ggggacgtcc aggccgacgg ctactcggcg ccgtacatct cggccatctg cgtgggatcg
     7381 gcggcctacg gcatcctggc gctgctgctc gcttggtttg ccgcgaggcg ggtgctcgcg
     7441 ctcgccgggg aggcccgcgc cgcgccggcg acggccgcca ccgtggccgt gggcctcggc
     7501 acgccgctgc tcttctacat gtacgtggcc cccggttacg cccatgcgac ctcggcgttc
     7561 gcggcatcgg ccttcgtgct cgcgtggctg gccgtccggg aggagtggtc cctgcgaggc
     7621 ctggccggcc tcggcgccct ggccgcactg atggcgatgg tccgcgagca ggacgcgttc
     7681 atcgtcgtgg ggccggcgat cgacctgctg tggacggtgg tcggcgagcg ccgctggcgc
     7741 ctgatcgtgc gcgccctggc gggcgcggca gtggcggcgg ctgcgtactt gccgcaggcg
     7801 gcggcatacc tcctcatcaa cgggcacctc ggcccctcgc gcctcgtgag ccggaagatg
     7861 acgtggacgg cgccgcacgc gctcgaggtc gtgtgctcga ccgagcacgg gctcatctgg
     7921 tggacgccgc tcgcgctcgc ggccatggcc gggatcgggc tgctcgccgc gggcgctggc
     7981 gcggcccctg gcgcggcggc ggactgcggg aaccggcgct cggcagcccg aatcggcgcg
     8041 ggccttgcgg cgatcgtgct gctgcaaatc tacatcgccg ggtcggtcga gagctggacg
     8101 gtggccggcg cgttcgggca gcgccgcttc gtcgcgctga cggcgccctt cgtgattggc
     8161 ctggcggtgg tcggcgcgcg gctcgcctcg gtgcgcgcgc gaaccgccgc agccgcgctg
     8221 gccgcggtgt gcgtctggtg gaacctgggg ctgatggtgc agttcggcgc cgggctgatg
     8281 gatcggcagc gcctcgaatt gcgccgcaac gcctacaata cgttcatcga ggttccgcag
     8341 cgtctgccgg agctggcgca ccgctacctg ttcgatcgcg ccagcttcta ccgcccggct
     8401 gcgccgggcc gggggccggg ctgatgcggc cggtcgctcc atgctgatca ggtacttcgc
     8461 cgacatccgc ttcccgctcg agcgggccaa cggcgtgcag accatggaga cctgccatgc
     8521 gctggcgcga cgggggcacc tcgtccacct ggttgtgcgc cgcgacagcg ccgacccgcc
     8581 tcgcgatccg ttcgcctact acggcctccc ctcgcatccc ctgctgtcgg tcgagcggct
     8641 gcgggtgccc gccggggagg cgccgcgccg cgcgatgttc atcgcgaaga gcctgcggcg
     8701 cgtgacgggc attccgcggg ccgacgccat cctgacgcgg gacctcgcgt tcgcagcgct
     8761 cctcgtccgc ctcccgcggc cgtggcgctc accggtggtc tacgagtcgc acggctacgc
     8821 gcccgccgtt gcggaggacc tgccggcgat ggtgtcgggg gctgcgccgc tctcgaccgc
     8881 caagcggcgc cgtctcgaag cgcgcgaacg cctcgtgtgg gccgccgccg acgggtacgt
     8941 cacgatcacg gcggcgctcg cgcgcgaact ggaggaccgg ttcggcgcca ggacgcggct
     9001 cgccgtgatt ccggatggcg cgaggctggc ccccatcgac ggaaccggcc ttcgcgcgcg
     9061 cgcggatggc gaggcgccgg ttgtcgccta cgccggccac ctgtacccgt ggaaaggcgc
     9121 ggacgtcctg gtcgaggccg tggcccgcct gccggaagtc cgggccgtcg tggtcggagg
     9181 catgccggga gaacctgatc tgccgcgcgt gtgcgcgctg gcggaccgga tcgcaccagg
     9241 gcgagtgcgg ttcacgggcc tggtcgaacc gccgcgcgtg ccggcgctgc tgcgcgacgc
     9301 cgacgtcctg gtggtgccca acacgcccgg ccgcgcatcg gcgtcctaca cgtccccgct
     9361 gaagctgttc gaatacatgg cctccggccg tccgatcgtc gcttccgatc tgccggcgct
     9421 cggcgaggtg ctcaggcggg acgagaacgc cgtgctggtg gaagccggaa acgccgaggc
     9481 cctcgcggcc gcactcgcgc gcgtgctcgg ggacgcgccg cttgcggcgc ggctggcggc
     9541 gcaggcccgc gaggacgcct cgggctggac ctgggacgcg cgcgcccggc gcctggaggc
     9601 cctgctcgac gcggtcgccg gaggcgccgc atgatctcgg atcggctcct gcaactggtc
     9661 cgctgccctg attgcggggg gcggctcggc ggccgtccgc cggggctggt ctgcgagtcc
     9721 tgcagccgcc cgttcgcgac ggacccggat ttcctcgtgc tccaccccgg catcacgttc
     9781 gccgagcaga cgaagtacct cgacgcagcc ctgcatgccg acgcgaggca cgagcgggtg
     9841 acacccccgc tgctcggcgc gcgcatccgc aacgacgtgc tccgctcgtt cctcgccccg
     9901 ggaccaggag acgccgtcat cgatctcggc tgcggcagcg gccggacgct ggtgtggaat
     9961 gccggcctcg gggcctacca ggcgggcatc gacgtgagcc cgtacttcgc ccgggaagcc
    10021 cgggcgcagg ccgacctcgt actcggcgat ctcaggcggc tgccgttcgc cgcgggcgcc
    10081 ttcacgaagg cgtcggcgct cgacgtgttc gaacacctct cgcgggacgc gctggccggc
    10141 gtgcttgcgg agatcgcgcg ggtgctcgca cccggcggcg cgctcttcgt ctacagccac
    10201 gtgcggaaga actcacggct ggccggaggc ctccggcaga tcaaccgcct ggcccgctgg
    10261 atggagcgcc gaggcctggt cgacctgtcg cgggaacggc tgcgaaaatc cgatcacctc
    10321 aatccgcttg cggacatccc cgatctcgag tcggcagtgg ccggcgcggg cttccggatc
    10381 gagagaatcc ggtactacac gccgctcgtg ggcgggttcg tcgagaacat cctcgtgcgg
    10441 gcgggagagc attggctcgc caagcgggcg gcggcgcgcg tggcggcggg cggcggcggc
    10501 gcgggccggg atgccgcggt gcgtgacgtg cgggccgccg tcaagcgcag ggccggcgag
    10561 tccggcccgg cgtactgggg attgcgcgcg gtcacgtgga tcatgaagat cgacctggtg
    10621 ctcttcggcc gcgtgcgttc gggcccgtac ttcgccctgc tcaggcacct gcctgccgag
    10681 ggcggcgcgc catgagaatc gtctactgcg cg
//
LOCUS       DAOSIF010000043        10675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6080_length_10675_cov_4.270056, whole genome shotgun sequence.
ACCESSION   DAOSIF010000043 DAOSIF010000000
VERSION     DAOSIF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10675
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6080_length_10675_cov_4.270056"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLT35_02750"
     CDS             <1..761
                     /locus_tag="PLT35_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28173.1"
                     /translation="AEPTGEFAHPPAPTAANMRQVATLARCFHADAGAALNVDGDRIG
                     FVTAEGAALSEEYSLALAAMARLRRRPGPVVTSLSTSAMVEALARACGQRVIRGPVGE
                     SQVVDLGLAEGAVLAGEGSGGVAVLPHGVAYDGLLALGLVLEEMATSGKTLAELAGSL
                     PTLHMRKRELPSPPNLVYRVIERFRRHHAHENPDCTDGVRLAWGDAWLHVRASGTEPL
                     LRIIAEAPDPARAEALVEEAAVFARRAAFGYDGS"
     gene            766..2121
                     /locus_tag="PLT35_02755"
     CDS             766..2121
                     /locus_tag="PLT35_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28174.1"
                     /translation="MAQRLSLKIGHSGVRGIVGETLTPQLVTSFAAAFGTYCGAGPIL
                     IGTDTRPSGDMLKQAAIAGLLSVGCTPIDAGVAPLPSLMLHVGEAGAFGGISVGGGPG
                     SPAWNALRFISAEGRALRANQAAELTDLYHQGVYPRVGALEMSDLRADASSEARHLAA
                     VTRAVDADRIRSRRFTVALAEGGGGAEGPARRMLEALGCDVVSLDPPAREAASEEAGT
                     EEASVARLADLVRGSGADAGFAIDGDAVRLAAVDERGTPLGRDASVALVVGRWLEHKA
                     GPVVVSVATSRMVDDAAARFGVAVHRSPVGEPNVVAAMQERGAELGGEGDGGVVVLPI
                     NACRDSFAAMAVILESAAVSGATLSGLRERLPRYAILRDRLLCPARDIAPSLRLVRNL
                     YRGERIDLIDGVQVRWPDRWLLVRPSTTEPVLRITAEAPSATEARALVDSVLDLLSPG
                     V"
     gene            complement(2194..3084)
                     /locus_tag="PLT35_02760"
     CDS             complement(2194..3084)
                     /locus_tag="PLT35_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOG28175.1"
                     /translation="MAQNEAPAARASAPATLQVSCVQLHWAKPLGFNLERTVHYIGAA
                     ARAGSRVVLFPEASLTGYYFPYAIGLEPAEVDGALDAVRAEARDRGIWVIAGTIRRTS
                     DRFLNLAHVIDPSGAIVHEYAKVHMAGRDEQQYCRGGDKLSLFEIDGVRCTLVICRDG
                     RHPELYRIPAMAGAQILFHPSNSTDEVEAVCWKRTSGRAQQPVGPNSKIFHCVANTIG
                     QSPDGKQTSSGSSFIREPNGLPLAEAGHYQEEMITAVLDLSRADRSYVRDSLAHPPFL
                     ASYWRQMVAAAEARKDVAPE"
     gene            complement(3139..4611)
                     /locus_tag="PLT35_02765"
     CDS             complement(3139..4611)
                     /locus_tag="PLT35_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG28176.1"
                     /translation="MTHKVSRRDFLVQSVVGGLSACGAFARTARAGPVGANDAVRVAI
                     VGVRSKGAHHIQMFKQVPGVRIAALCDIDEGILAKAADGLAAEGVQVAREVDVRRLLE
                     RHDVDAVVIATPNHWHALMAVWACQAGKDVYVEKPVSHSVWEGRKVVEAAAKYGRIVQ
                     AGTQSRSDEALIELAALLRGGGLGKILCARGFCYKRRDTIGRVNGPQPIPPGVHYDLF
                     CGPAPLEPLRRKELHYDWHWVWPTGNGDIGNQGIHEMDMCRWMLGEEGLPRRVLSIGG
                     RYGYVDDATTPNTQIAVYDYPSAPIIFEVRGLPQQAGANYMDQYRGVRVGIVIECEQG
                     YFAGGSGGGAIYDQAGKRVRGLPSTGGGAHVENFIAAVRSRKPEQLKAPVLGAHYSSA
                     LCHLGNISYRLGTEMSAAEVRARAASRADVAEAFERFVSHLAANGVDAATAAVTSGPV
                     LDLVPADERFATQSAFDMGAWANRLLRDEYRPPFVVPEKV"
     gene            complement(4864..6501)
                     /locus_tag="PLT35_02770"
     CDS             complement(4864..6501)
                     /locus_tag="PLT35_02770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG28177.1"
                     /translation="MAAPRLVRVLRALLAEPLAAGAEAALDRWRAVVLLALAGAWLTV
                     TMASAQRKPFWHDEVVTIVLSRLPSCGRVWTATRDGADLAPPLNTLVTRVVHETVGVG
                     PVSTRIAAMAGTLLMVGLLFAFVRRRAGTSLGAAAVVLVSCTAAFRYGYEARGYGLLL
                     GFFMLALYSWSEAAGGRHRRLFLPLLTIALVGACWSHYFGAFALAPVAAGEIVRAIGR
                     RRPDAGVCTAAAVSVACCVPLLVLVRSAAAQSAHFWGRAADVGVAAAYAFVLHELLGP
                     LVAGAALLALVIAVAGRWTRARADGRGMPAHEIAAVAACLLLPVIEIAAASAVGGAFV
                     PRYALPASIGAALAMPLAVWYLSRGATLARLAFALVLVAGFAATSAESLIRPGAEPAS
                     PADTRPVLMSALRGPRPVAVTGGLMFLQLWYYSPPALRGRLWFLADPREAAAFTGSDT
                     IDRGLLALARWAPLNVADYPGFTSTHPEFGVYAAGSGWLLDRLIRDAWELESAAAEPG
                     GQLFVAKRVGVSGSGSGAGTPSAPDARRMSAAPSELF"
     gene            6798..8150
                     /locus_tag="PLT35_02775"
     CDS             6798..8150
                     /locus_tag="PLT35_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28178.1"
                     /translation="MTPHAHGGTRATAAGLCLALFVAYNANGREIGSYDSLPTKLAAR
                     EFLLRGTLKLNYAVGALPELAQRWGFVLGKDGSYRSVYSPAPSIIAAGLTWPFSKSGI
                     VNLRAPRAPALMAKATASLLVALAAALAFISARQRLPQGRALFLAIGLALGTGYWYMA
                     SQTLWQTETAVLGLALAVAAFAAPRDLVNIRAAIAIGAGLGLAAVARPQLAPLVAVLL
                     AGTWVRAPLRTALVATAITLAFGIALGAANLRWFGDPRGAQAWLQDVNARIHGTRSMF
                     VAEPGGFIGLLVSPSRGLIVFSPVVLVAFAGVPRAFKEGWRSPLPWCALAIASQYVLY
                     GSYAVWWAGHTYGPRYLVDVLPVAVPLAAVAMSAPRPGRVARIAAGAALAWSIGVAAV
                     GAFCYPHGQWNSQPTDVDRDHSRLWSVSDNQIARTLAAGLSPQNFRLFNPASIRVVPE
                     "
     gene            complement(8290..8940)
                     /locus_tag="PLT35_02780"
     CDS             complement(8290..8940)
                     /locus_tag="PLT35_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HOG28179.1"
                     /translation="MTEGLRRSEAGPEALLLEAAAWRLIALLFECPGPGWQAHVAALG
                     RDCRDPALQAAAHAAQTEASEGLYHSLFGAGGPVSLREVTYAGGMRPGYLLSELGAYY
                     EAFAYRPGSPEPPDHLSVEAGFVSYLAMKQAYAAASNDPEGAGITADAMAAFCAEHLA
                     GMAEPVARALEAGGPPYLALAGRALLERAGPPRGSPLPLGGAGSDNECGQAECGTV"
     gene            complement(8937..9800)
                     /locus_tag="PLT35_02785"
     CDS             complement(8937..9800)
                     /locus_tag="PLT35_02785"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HOG28180.1"
                     /translation="MRARRQLLAASVLTAAGLAAACAKPPAPVQEVVAARTAVLPAAP
                     DEAAWNAAPEHVAKMIPQDLVEPRLLAPSTAEVRIRALTNGETIAFRLNWADATKSDT
                     PGPAQMVDACAVQIPQQNGPQPPAPQMGEIGRPVDVVYWRADWQAIVNGRGDSIRDLY
                     PNAYVDPYPFNARPLVPGSPEQADMARRYAPARALDNNRSGPRTTPVEELVAQGPGTS
                     EPAAPRGSTGRGIWAAGSWMVVIARPWPQDVRDGQRTSVAFAVWQGSQHEAGARKMRT
                     GWIPLLAGGAR"
     gene            complement(9871..>10675)
                     /locus_tag="PLT35_02790"
     CDS             complement(9871..>10675)
                     /locus_tag="PLT35_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOG28181.1"
                     /translation="LGWRPEQQDYSYPNVGEDDCAGQVDHGALIQVPHLSWFFYLARI
                     CNHCTYPACLAACPRGSIYKRQEDGIVLVDQGRCRGYQECVRGCPYKKVFFNPMTGTS
                     EKCIACFPKIEQGVQPQCFVNCIGKIRLAGFLSAADQAKAENPIDYLVHLRKVALPLF
                     PQFGLEPNVYYIPPIHAPLAFNEQLFGPGAGAAMAAYRGAPADRDLAGLLCLFGSTPS
                     LVARWRRRGDLVSGLDERASEIVRVPLLEPVHVRPAVDPTRQVVRTNCP"
BASE COUNT         1462 a         3893 c         3817 g         1503 t
ORIGIN      
        1 acgcggagcc caccggcgag ttcgcgcacc cgccggcgcc gacggcggcg aacatgcggc
       61 aggtcgccac cctcgcgcgc tgcttccacg ccgacgccgg cgccgccctc aacgtggacg
      121 gcgaccggat tggcttcgtc acggcggaag gcgcggcgct gtcggaggag tactccctcg
      181 cgctggcggc gatggcccgc ctgcgccgca ggccggggcc ggtcgtgacg agcctctcga
      241 cctcggcgat ggtcgaggcg ctggcccggg cctgcgggca gcgcgtcatc cggggccccg
      301 tcggcgagag ccaggttgtc gatctgggcc tcgcggaagg cgccgtgctg gccggcgaag
      361 gcagcggcgg ggtggccgtg ctgccgcacg gcgtcgccta cgacggcctg ctcgccctcg
      421 gcctggtgct cgaggagatg gcgacgagcg gcaagaccct cgccgaactg gccgggtccc
      481 tgccgacgct ccacatgcgg aagcgcgagc tgccgagccc gccgaacctc gtctaccggg
      541 tgatcgagcg tttccgccgg caccatgcac acgagaatcc cgactgcacc gacggcgtcc
      601 gcctcgcctg gggcgacgcg tggctgcacg tgcgcgcgtc gggcacggaa cccctcctcc
      661 gcatcatcgc cgaggctccg gacccggcgc gggccgaggc cctggtggaa gaggccgcgg
      721 tcttcgcccg ccgggccgca ttcgggtacg acgggagctg agtcgatggc gcagcgcctc
      781 tcgctgaaga tcgggcattc gggcgtgcgg ggcatcgtcg gcgagacgct gacccctcag
      841 cttgtgacga gcttcgccgc ggccttcggg acgtattgcg gggccgggcc catcctcatc
      901 gggaccgaca cccggccgtc gggcgacatg ctgaagcagg ccgccatcgc gggcctgctg
      961 agcgtgggct gcacgccgat tgacgcgggc gtcgcgccgc tcccgtcact gatgctgcac
     1021 gtcggcgagg ccggcgcctt cggcggcatt tcggtcggcg gcggccccgg ctcgccggcg
     1081 tggaacgcgc tgcgcttcat cagcgccgag ggccgcgcgc tgcgggccaa ccaggcggcc
     1141 gagctgacgg acctctacca ccagggcgtc tatccgcgcg tcggcgcgct cgagatgtcc
     1201 gatctccgtg ccgacgcgtc ctcggaggcg cgccacctgg ccgccgtgac gcgcgccgtc
     1261 gatgccgatc ggatccgatc gcgccgcttc acggtcgcgc tcgccgaggg cggcgggggc
     1321 gcggaagggc cggcgcgccg gatgctcgag gcgctcggct gcgacgtggt ctccctcgac
     1381 ccgcccgcgc gggaggccgc gtcggaggag gccgggaccg aagaggccag cgtcgcgcgc
     1441 ctggccgacc tcgtgcgcgg ctccggggcc gacgccggct tcgccatcga cggcgacgcc
     1501 gtgcggctgg ccgccgtgga cgagcgcggc acgccgctcg gccgcgacgc gtcggtcgcc
     1561 ctggtcgtcg ggcgatggct cgagcacaag gcggggcccg tcgtcgtcag cgttgcgacg
     1621 agccggatgg tggacgatgc ggcggctcgc ttcggcgtcg ccgtccatcg ctcgcccgtc
     1681 ggcgagccga acgtggtggc ggccatgcag gagcgcggcg ccgagctcgg cggcgaaggc
     1741 gacggcggcg tggtggtgct gccgatcaac gcctgccggg actccttcgc ggccatggcg
     1801 gtcatcctcg agtcggcggc ggtgtccggc gcgacgctga gcggcttgcg cgagcggctg
     1861 ccccgctatg caatcctccg cgaccgcctg ctgtgccccg cgcgcgacat cgcgccatcg
     1921 ctgcggctgg tccggaacct gtaccgcggc gagcggatcg acctcatcga cggcgtgcag
     1981 gtgaggtggc cggaccgctg gctcctcgtc cggccgtcga cgaccgagcc cgtcctccgc
     2041 atcacggccg aggcgccgag cgcgaccgaa gcgcgcgcgc tggtggacag cgtcctcgat
     2101 ctcctcagcc ccggcgtctg aaggggtcgg gacgattttc agcacgctgc gcccggcagc
     2161 gacaagatcg tcatcgtcag caggccaggc ccgctactcc ggcgcgacgt ctttgcgcgc
     2221 ctccgcggcg gccaccatct gccgccagta ggaggcgagg aacggcgggt gcgcgaggct
     2281 gtcgcggacg tacgaccggt ccgcgcggga gagatcgagc accgcggtga tcatctcttc
     2341 ctggtagtgg cccgcctcgg cgagcgggag gccgttcggc tccctgatga acgacgaccc
     2401 gctcgacgtc tgctttccgt cgggcgactg gccgatcgtg ttcgccacgc agtggaagat
     2461 cttcgagttc gggccgacgg gctgctgagc gcgtcccgac gtgcgcttcc agcagacggc
     2521 ctcgacctcg tcggtgctgt tcgatgggtg gaacaggatc tgcgcgccag ccatcgccgg
     2581 gatgcggtag agctcgggat gccggccgtc gcggcagatg acgagcgtgc agcggacgcc
     2641 gtcgatctcg aacagcgaca gcttgtcgcc gccgcggcag tactgctgct cgtcgcggcc
     2701 ggccatgtga accttcgcgt attcgtgcac gatcgcgccg gacgggtcga tgacgtgggc
     2761 gaggttcagg aaccggtcgc tggttctccg gatcgtgccg gcgatcaccc agatgccgcg
     2821 atcgcgtgcc tcggcccgca cagcgtcgag cgccccgtcg acctccgccg gctcgaggcc
     2881 gatcgcatag gggaagtagt agcccgtcag cgacgcctca gggaagagca ccactcggct
     2941 gccggccctg gccgcggcgc cgatgtagtg caccgtccgc tcgaggttga acccgagcgg
     3001 cttcgcccag tgcagctgga cgcaggacac ttgcagggtc gccggcgcgg atgcacgcgc
     3061 ggccggggct tcattctgtg ccacggactc ctccaggatg ggcccgaacc gccggacgcc
     3121 cggcgcggcg cctctcttct acaccttctc gggaacgacg aagggcggcc ggtactcgtc
     3181 gcgaaggagc cggttggccc acgcgcccat gtcgaacgcc gactgcgtgg cgaaccgctc
     3241 gtccgccggc acaaggtcca gcaccggccc cgacgtcacg gcggccgtcg cggcatcgac
     3301 gccattggcc gccaggtggc tgacaaagcg ctcgaaggcc tccgccacgt cggcccgcga
     3361 ggcggcccgg gccctcactt cagcggccga catctcggtg ccgaggcggt acgagatgtt
     3421 gcccagatgg cacagcgccg acgagtagtg ggcgcctagg accggcgcct tcagttgctc
     3481 gggcttccgg ctgcggaccg ccgcgatgaa gttctcgacg tgcgcgccgc cgcccgtgct
     3541 cgggagcccc ctcacgcgct tcccggcctg gtcgtagatc gcgccgcctc ccgacccgcc
     3601 ggcgaaatac ccctgctcgc actcgatgac gatcccgacc cgcacgccgc ggtactgatc
     3661 catgtagttg gcgcccgcct gctgcggcag cccgcgcacc tcgaagatga tcggggccga
     3721 cgggtagtcg tagacggcga tctgcgtgtt gggcgtcgtc gcatcgtcga cgtagccgta
     3781 gcgcccgccg atgctgagga cccgccgcgg caggccttcc tcgccgagca tccaccggca
     3841 catgtccatc tcgtggatcc cctggttgcc gatgtcgccg ttgccggtgg gccagaccca
     3901 gtgccagtcg tagtgcagtt ccttgcgccg caggggctcg agcggggccg gtccgcagaa
     3961 caggtcgtag tgcaccccgg gcgggatggg ctgcgggccg ttcacccggc cgatcgtgtc
     4021 ccgccgcttg tagcagaagc cgcgggcgca gaggatcttt cccagcccgc cgccgcgcag
     4081 cagggcggcc agctcgatga gggcctcgtc cgatctcgac tgcgtgcccg cctgaacgat
     4141 gcggccgtac ttcgccgcgg cttcgaccac cttgcgaccc tcccacacgc tgtgggagac
     4201 gggcttctcc acgtacacgt ccttgcccgc ctggcaggcc cagacggcca tcaatgcgtg
     4261 ccagtggttg ggcgtcgcga tgaccacggc gtccacgtcg tgccgctcca gcagccggcg
     4321 gacatccacc tccctggcca cctggacgcc ttcggcagcc aggccgtcgg cggccttcgc
     4381 caggatgcct tcgtcgatgt cgcagagcgc cgcgatgcgg acgccgggca cctgcttgaa
     4441 catctggatg tggtgggcgc ccttgcttct cacgccgaca atcgccacgc gcacggcgtc
     4501 gttggccccg acggggcccg cccgcgccgt ccgcgcgaac gcgccgcacg ccgaaagacc
     4561 gccaaccacg ctctgcacca ggaagtcacg gcggctgacc ttgtgtgtca cgggcgttcc
     4621 tcacctgccg tctccgccat agtaccgccg atcgggcgcg agggccacgc gccggcggcg
     4681 agtcggccac gccggctgcg cgcgcttcat tcgggctcct ccggctggcc cttgccgggt
     4741 caggagggcg gctctgtgcc ggcgggcgcg tccgccgccg ggactgcgag agtgatggcc
     4801 gctggcaggg ccgccgcccc gaggcatcgc aggcgccccg gcgagacggg cctggcgcgc
     4861 tcgtcagaaa agctccgagg gcgcggcgga cattcggcgg gcatcagggg ccgatggcgt
     4921 tcccgcaccg ctccccgagc ctgagactcc gacgcgtttc gcgacgaaga gctgccctcc
     4981 aggctcggca gcagccgatt ccagctccca ggcgtcgcgg atgagccggt ccaggagcca
     5041 gcccgacccg gcagcgtaca cgccgaactc cgggtgcgtc gacgtgaagc ccgggtagtc
     5101 ggcgacgttc aacggcgccc agcgggccaa tgccagcagg ccccggtcaa tcgtgtccga
     5161 accagtgaag gccgcggcct cgcggggatc cgccaggaac cagagtcgcc cgcgcaaggc
     5221 gggcggcgag tagtaccaca actgcaggaa catcaagccg ccggtcaccg caacgggcct
     5281 cggcccgcgc agggccgaca tcaggacggg gcgcgtgtcg gcggggctgg caggctccgc
     5341 gcccggtctg atgaggctct ctgcgctcgt cgcagcgaaa ccggcaacca gcaccagcgc
     5401 gaaggcaagc cgagccagcg tcgcgccacg gctaaggtac cagacagcca gaggcatcgc
     5461 gagcgcggcg ccaatcgagg ccggcagcgc gtagcgaggc acgaaggccc cgccgaccgc
     5521 cgaggcggct gcaatctcga tcaccggcag caggaggcat gcggcgactg cggcgatctc
     5581 gtgcgccggc attcccctgc cgtccgcgcg cgcgcgcgtc catcggcccg ccacggcgat
     5641 gacgagagcg aggagcgccg cgccggcgac cagcggaccg agcagctcgt gcagcacgaa
     5701 cgcatatgcg gcggcgacgc cgacgtcggc cgcccggccc cagaagtggg ccgactgcgc
     5761 cgccgcgctc cgcaccaaca ccagcagggg cacgcagcac gccactgaca cggcggccgc
     5821 cgtgcatacc cccgcatcgg gccgcctgcg accgatcgcg cggacgatct cgcctgccgc
     5881 aaccggcgcg agcgcgaacg cgccgaaata gtggctccag caggcgccga ccagggcgat
     5941 ggtgaggagg ggcaagaaaa gcctgcggtg cctgccgccg gccgcttcac tccatgagta
     6001 cagcgccagc atgaagaacc cgagcagcag cccgtagcct ctcgcctcat acccgtaccg
     6061 gaaggcagcc gtgcacgaga cgagcaccac cgccgccgcg ccgaggctgg tgccggcacg
     6121 cctgcggacg aacgcaaaga gcaggccgac catcagcagc gttcctgcca tcgccgcgat
     6181 gcgcgtgctc acgggcccga caccgaccgt ctcgtgcacg actcgcgtga ccagcgtgtt
     6241 cagcggaggc gccagatcgg cgccgtcccg tgtggctgtc cagacccgcc cgcaggacgg
     6301 aaggcgggag aggacgatcg tgacgacctc gtcgtgccag aacggcttcc gctgcgcgga
     6361 tgccatcgta accgtcagcc acgctcctgc cagggcaagg aggacgactg cgcgccatcg
     6421 gtcgagcgcg gcctctgcgc ctgcggccag cggctctgca agcaacgcac ggagaacgcg
     6481 gaccagccgc ggggcggcca tcggctgctc cttcacaccc ggcggctgcg tggtggatcg
     6541 ggcgtcacgc cggaatcttc cgcggatgca cgccggaagt caacgacgcg accgcacgag
     6601 gtccgtcgcg ccggcggccg tcccgcgcgc gtggcggcgt cgacgcggga ttggcgcggc
     6661 tggctcccgt gcggtcggcg cagatccctt gcccggagct tgtcgccagc gcaggcttcg
     6721 atggcggggc cgcctccctc gacaagttga cgctccgtgt atgatcggcg cttggacctt
     6781 gccaagcggt gcccagcatg acgccccacg ctcacggcgg aacccgagcg acggcagcgg
     6841 ggctctgcct cgcgttgttc gtcgcctaca acgccaacgg ccgtgagatc gggagctacg
     6901 acagcctgcc caccaagctg gccgcgcggg aattcctgct ccggggaacg ttgaagctga
     6961 actacgcggt cggggcattg cccgagctgg cccagcgctg gggattcgtg cttgggaagg
     7021 atggctccta ccgttccgtg tactcgccgg cgccatccat catcgcggcc ggcctcacgt
     7081 ggcccttctc gaagtcgggg atcgtcaatc tgcgagcccc gcgcgcgccg gcgctcatgg
     7141 ccaaggcgac cgcctctctg ctggttgcgc tggcggccgc cctggcgttc atcagcgccc
     7201 ggcagcgact gccgcaaggc cgggcgctgt tcctggccat cggcctggct ttgggaaccg
     7261 gctactggta catggcaagc cagacgctat ggcagaccga gaccgccgtc ctcggcctgg
     7321 cgctggccgt ggccgccttc gcggcgccac gggacctggt gaacatcagg gcggcaatcg
     7381 ccatcggagc cggtctgggg cttgccgccg tggcgcgccc gcagctcgcg ccattggtcg
     7441 ccgtactgct cgccgggacg tgggtgaggg cgccgctcag gaccgccctg gtggcgactg
     7501 ccatcacgtt ggcattcggc atcgcgctcg gcgccgccaa tctgcggtgg tttggggatc
     7561 cgcggggcgc gcaggcctgg ctccaggatg tcaacgcccg gattcacggc acgcgctcga
     7621 tgttcgtcgc ggagcctggc ggcttcatcg gcctgctcgt gtcgcccagc cgagggctga
     7681 tcgtgttcag cccggtcgtg ctcgtcgcct tcgccggcgt gccgcgggcg ttcaaagaag
     7741 gatggcggtc gcccttgcca tggtgcgcgc tggcgatcgc gtcgcagtac gtcctttacg
     7801 ggagctacgc cgtgtggtgg gccgggcaca cctacgggcc gcggtatctc gtcgatgtgc
     7861 tccccgtcgc ggtccctctt gcggccgtgg cgatgagcgc gccgcgaccc gggcgggtag
     7921 ccaggatcgc cgcgggagcc gcgctggcgt ggtcgatagg cgtcgccgcc gtcggcgcgt
     7981 tctgctatcc ccacgggcag tggaacagcc agccgactga cgtcgatcgc gatcactcgc
     8041 gtctctggag cgtgtccgac aaccagatcg cgcgcacctt ggcagcgggg ttgagcccgc
     8101 agaacttcag gctgttcaac ccggccagca tccgtgtcgt gcccgagtag ccgcggggcg
     8161 cctccccgcg ctggccggga agatgcactt gtccttcttc atcggctgga ggatttccgc
     8221 cagcggcgtg ccgccctgtc cgcccggggc cctgccgccg gcggcatgcc ggcgcggctg
     8281 cggtcccggc tacaccgtcc cgcactccgc ctggccgcat tcattgtctg acccggcgcc
     8341 gccgagcggc agcggactgc cgcgcggcgg gccggcccgt tcgagcagtg cgcggccggc
     8401 gagggcgagg tacggcgggc cgccggcctc gagcgcccgc gccacaggtt ccgccatccc
     8461 ggccaggtgc tcggcgcaga aggcggccat ggcgtcggcc gtgatgccgg cgccttcggg
     8521 atcgttcgac gccgccgcat acgcctgctt catcgcgagg tacgacacga agcctgcctc
     8581 gaccgagaga tgatcgggcg gttcgggcga accgggccgg tatgcgaagg cttcgtagta
     8641 cgcgccgagc tcggacagga ggtacccggg ccgcatgcca ccggcgtagg tgacttcgcg
     8701 cagcgagacc ggcccgccgg cgccgaacag cgagtggtac agcccctcgg acgcttccgt
     8761 ctgagcggcg tgcgctgcgg cctgcagcgc cgggtcgcgg cagtcccggc cgagggccgc
     8821 gacgtgcgcc tgccagcccg gtcccgggca ctcgaacagc agggcgatca gccgccaggc
     8881 ggcggcttcg aggagaagcg cctcggggcc ggcctccgag cgccgcaggc cctcggtcac
     8941 cgcgcgcccc cggccaggag tggaatccag cccgtccgca tcttgcgggc gcccgcctca
     9001 tgctgcgagc cctgccacac ggcaaacgcg acgctggtgc gctgcccgtc cctcacgtcc
     9061 tgcggccagg gtcgcgcgat cacgaccatc caggagccag cggcccagat gccccggcct
     9121 gtcgatcccc gcggcgctgc cggttcggag gtcccggggc cctgggccac gagctcctcc
     9181 accggcgtcg tcctcgggcc gctgcggttg ttgtccagcg cccgggcggg cgcgtaccgg
     9241 cgcgccatgt ccgcctgctc cggcgacccg ggaaccagcg gcctcgcgtt gaacggatag
     9301 ggatccacgt aggcgttcgg gtacaggtcg cgaatcgagt ccccgcggcc gttgacgatg
     9361 gcctgccaat cggcccgcca gtagaccacg tccaccggcc tgccgatctc gcccatctgg
     9421 ggggcgggcg gctgcggtcc gttctgctgc gggatctgca cggcgcaggc atccaccatc
     9481 tgcgccggcc cgggcgtgtc gctcttcgtc gcgtccgccc agttcagccg gaaggcgatt
     9541 gtctcgccgt tcgtcagcgc ccggatgcgc acctctgccg tcgagggggc gaggagccgg
     9601 ggctcgacga ggtcctgcgg gatcatcttc gcgacgtgct cgggcgcggc gttccaggcc
     9661 gcctcgtcgg gcgcggccgg cagcacggcc gtcctggcgg cgacgacctc ctggaccggc
     9721 gccggcggct tcgcgcacgc tgccgccagg ccggccgcgg tgaggacgga ggctgcgagc
     9781 agctggcgtc gggctctcat gggctcctcg actcgacagc gcccggggcg gcagggctgc
     9841 tccgcgggag cagcccggcc cgccgccaga ctaaggacaa ttcgtgcgaa cgacctgccg
     9901 cgtcgggtcg acggcaggcc tcacgtgaac cggctcgagc agaggcaccc tcacgatctc
     9961 cgaggccctc tcgtccagcc cggacacgag atcgccccgc ctccgccatc gcgccaccag
    10021 cgacggggtc gagccgaaca ggcacagcag tcccgcgagg tccctgtccg cgggcgcgcc
    10081 gcgataggcc gccatcgccg cgccggcgcc ggggccgaac agctgctcgt tgaacgcgag
    10141 cggcgcgtgg atcggcggga tgtagtagac gttcggctcc aggccgaact gcgggaacag
    10201 cggcagcgcc accttccgca agtggacgag gtagtcgatg gggttctcgg ccttcgcctg
    10261 gtcggccgcc gacaggaaac ccgcgaggcg gatcttgccg atgcagttca cgaagcactg
    10321 cggctgcacg ccctgttcga tcttcgggaa gcaggcgatg cacttctcgg acgttcccgt
    10381 catcgggttg aagaagacct tcttgtacgg gcacccccgc acgcactcct ggtatccgcg
    10441 gcaccggccc tggtcgacca ggacgatgcc gtcctcctgc cgcttgtaga tcgagccgcg
    10501 cgggcaggca gccaggcagg ccgggtaggt gcagtgattg cagatcctgg ccaggtagaa
    10561 gaaccagctc aggtgcggca cctggatgag ggcgccgtga tcgacctgcc ccgcgcagtc
    10621 gtcctccccg acgttggggt acgagtagtc ctgctgctcc ggccgccagc ccagt
//
LOCUS       DAOSIF010000044        10671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6086_length_10671_cov_5.166635, whole genome shotgun sequence.
ACCESSION   DAOSIF010000044 DAOSIF010000000
VERSION     DAOSIF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10671
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_10671_cov_5.166635"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLT35_02795"
     CDS             <1..307
                     /locus_tag="PLT35_02795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28182.1"
                     /translation="RIGGGTRLKILQAMALGTPVVSTSKGAEGLAVTPGRDILIGDSP
                     DQFAAHVLRLLADAELGTRLAAAARNLVRERHTWERSGERLHEAVAEAVSAWRVAGR"
     gene            304..1989
                     /locus_tag="PLT35_02800"
     CDS             304..1989
                     /locus_tag="PLT35_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28183.1"
                     /translation="MNHAQGRVGTAARFALVSAAVILVTGLPYLYGFAAAGPDRVFTG
                     LMYDVPDHAQYWSWVTASRESLFISNTMTPEANPPIFTNPMMWALAQVQRAFGLSFPA
                     LFQVWRFAAVFSLVAALWWFVSVMVPERARRRTAFWLSLAGSGLGWALIVGKVALRLP
                     DAPYPVDIYTIEPNTFWGLLAYPYLPLAQSLLLASVVCVWRAHKRPSVPAFFLAGLAS
                     LALALVHAYDLIVLYAVIGAFGLALLARDRRLPLALAGAGLTVAACSAPMAWYFQSLT
                     SSNPLWRSILAQYSGAGVWTPRHVHLIVLMGLPLILAVPGFFRRGPRTDERLLVSAWA
                     AVGLVLIYLPVVFQIKMLGGWQFPIAALAAGAWHESVEPWISRKFPRRVGGVSAVTAA
                     RALLLLAVLPTNVYLYAWRFVELRRQAPPYYLHRDEAAALDWLRHHAAPEDVVLAPEA
                     IGQFVPNYGESRAYLAHWAMTNRYHERVARVRAFFDPDVVDAWRAQLLRQEGVTLVLR
                     AGSVPGLPRLYDPGGSPLWDALFQSPRAQIYRLRPAPPVEVAEQGSPGGRGVR"
     gene            1986..2744
                     /locus_tag="PLT35_02805"
     CDS             1986..2744
                     /locus_tag="PLT35_02805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG28184.1"
                     /translation="MSAVNQLGFYLRSKTRSPWRYALGETLQALLAWVPGIVGIGLRA
                     IAYKVVLKADGLPAIEDHVRLVRCEDIRIGRSVYIDHGVYLHGGQGGLEIGDGSWLMA
                     GCRLHVFNFRGISHAGIRIGRRTFLGEETLMRGQGGITVGDNVLFAPRVQVLAVDHVM
                     ADPARPIMDQGITARGIVIDDGCWIGAGAVVLDGVTIGRGSCVGAGAVVTASVPPHSL
                     AVGVPARVIRNLRDNPLAAPDAAVYFGGMDELRA"
     gene            3085..3351
                     /locus_tag="PLT35_02810"
                     /pseudo
     CDS             3085..3351
                     /locus_tag="PLT35_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            3370..3993
                     /locus_tag="PLT35_02815"
     CDS             3370..3993
                     /locus_tag="PLT35_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28185.1"
                     /translation="MAAAGPRPRRPDLLFVGGLHWPPNADAVAHFARDIWPLVRREAP
                     DATLTVVGRADAPVAQALRRIDGIRLAGHVPDVAPYFERARAFVVPLRAGGGMRVKIL
                     EALARGLPVVSTAIGVEGIDAAHGVHLLVADDPGSFARAVSQVVGNDGLAAELAAAGR
                     RLVLERYDRSVVGEKLRRDLRECGGQLGRPTDAEQGRQKSAIRASFD"
     gene            4259..5470
                     /locus_tag="PLT35_02820"
     CDS             4259..5470
                     /locus_tag="PLT35_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG28186.1"
                     /translation="MAGSRTANGLSICIPVLNEEGSIGETLERCLALAPALGAIGLDG
                     LEIIAVDDGSTDRTAERVREHPGVRLIQHPVNRGYGAALKTGFAAARYDVIAFLDADA
                     TYPPEEFPALCQPIVDRKADLVIGSRMLGADSEMPLVRRIGNAFFASLLSLVGRSSVT
                     DSASGMRVFRREALGLLSPLPDGLNLTPVMSTRAVHERLNVVEVPITYRERVGRSKLS
                     VTKDGLRFLKTIVWTTLTYNPVRIFGAAGLAGIGLAAVIGLALTAMRLSGVTSLGPIG
                     AFSVFAAVVCAVAGVSVFALGASFNYLVSLFYKRPIRQGLFRKPLFEVPIERWFLPIG
                     LAAFAAGASMSVASLALAVGGWAIERLWLYLSASSLLILVGLHLTAWWLVMSVLHELS
                     AGGRTEEASRG"
     gene            5463..7115
                     /locus_tag="PLT35_02825"
     CDS             5463..7115
                     /locus_tag="PLT35_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG28187.1"
                     /translation="MGERQPEAASEARNVGRKTRLIVLPPAHPNTDAIARDSAIARGE
                     ASKVDVLLVNPPSPDGAVWIRSQHRVGRRSRENMLWPQVSLAQMAALLQPTYSIRIID
                     AIAERMDYRAFEDILRRERPRYYVTQLTGPTLQSDMYGAFLAKANGATTIAFGTHITP
                     MPTETLRPFPALDYGLRGEPDLTLCDLIDHLEGRQFDRPALLEPLFRKHDPLYQPRAL
                     ATLPDGSPDLSGVRGLVWRRGQEIVVNPDRPFIADLDDLPMPLHHLLPFNRYLMPLLK
                     GPFTFIVTSRGCPAGCTFCIKHVSYGPTMRMRSPSKLVEELQVLDTLGVHNIHMYADL
                     FTVNRDQVVGLCRGILEAGLRIRWTCNSRVDFVDREMLQLMARSGCHFISWGLESGNA
                     KILAHARKGVDPDRARQSLTLAHEAGIRNWGYFIIGLPGETEETIRQTIAFSKSLPLD
                     IALFHIAAPHPGTPFFHEVVRNGWFRKGVRWEEVDMDESTVLDYPGLPAERLEYWQKR
                     AFREWAMRPGPMLTYLRMVLSDTRTVKSALSVGLQHLGWAKR"
     gene            7112..8788
                     /locus_tag="PLT35_02830"
     CDS             7112..8788
                     /locus_tag="PLT35_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG28188.1"
                     /translation="MIGRRAPALAPTDLPVLFLLCSALAAVWPAFDRASGWRGAAAVA
                     AGALVYAAASRLRAAGPAWRALGGASVVAGAALGAYFIFQFPRLGYAEKSAVVERAAA
                     WLGSGAPAFGHWAPDRNSVATCLEGLVFLGVALASTRKGRLARVLWGAAVAIAAAAIA
                     LSASRGAALAVAAAGTVWAAMRLKPVAARPALASFLVALPLLSGLVWLAAVVWEPGAA
                     AGLETVSPALDRPDRVALYQQSAWLIRDAPFTGIGIGSQFAMSLSRYALLIQVPFLTY
                     SHNLYLETWLELGLAGAAALVWLVAAYAAGFKAIPQIRHGALAQGAGCGVLAVLLHGL
                     VDARPFVDSWSGLPAFVLLGLFASAVSAAETPPMRRRRRRVSAAAVCGFILCALIAVR
                     PAASSWHANLGAVAQATAELSADASDAERRTALQEAERRFHQALGGVPEQVTANLRLA
                     LIDMDDGRFALAVTRAETAWRGAPNTLRTRKAFGLASMWTGDLDRARVLLESVPGIVA
                     ELNAWGWWRQDRQEPSLALRAYRTSLLLDPGQTDVRRAAESLARAASGDR"
     gene            complement(8846..>10671)
                     /locus_tag="PLT35_02835"
     CDS             complement(8846..>10671)
                     /locus_tag="PLT35_02835"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOG28189.1"
                     /translation="AFRRAQTAVRRLLGGSAPPAAAQAAPADQASPLVDALLARLSVR
                     PIENSRLVDIVFQSQDPALAAKVANALARLAIDQDLESRYQSARHASEWLEKRLSEQR
                     AAVDASEAALQKYREEHGAVALDDRQNIVVQKLADLNAALTRAKTDRLAKEGLYTQLA
                     ALQGSQAALDTFPLILSNPYIQQLKAQVADLQKEHSQLAERYGERYPEMVRVRMALQA
                     AESRLQSEIAKTVEAVRNDYLAAQAQERSLTAALEAQKRQALDLGRVGIEYGSLERDA
                     ASNRQVLDSLLQQAKQTGLVAAVSSSNLRVVEAAVRPTAPVRPQRGRSLFLSLLGAAV
                     LALGLVFGAELADTRLKSPEEIRAYLDLPSLGLIPAVESKVLDHEMPLVGKRSSADFN
                     ESMRRLRTGLMLAAAKRGATSIAITSTSPQEGKTAMACNLAVVLAQADRHVLLVDADM
                     RRPQVHEIFGLRQQPGLSEVLASRAHPGQSICRGVERGLDVLPSGEAPPNPAELLILP
                     LFRQLIERAGSDYDYVIIDCPPVMAVTDATLIADAVSGVVFVVGADATARGAAQAALG
                     ELDSSDGKILGAVLNRVQLRRNRYYYARYYNPEYERYYRQG"
BASE COUNT         1401 a         3808 c         3764 g         1698 t
ORIGIN      
        1 gaggatcggc ggcgggacgc ggctgaaaat cctccaggcg atggctctcg gaacgccggt
       61 cgtgtcaacg tccaagggcg ccgaaggcct ggcggtgacc cccggccgcg acatcctgat
      121 tggagactcc cccgatcagt tcgcggcgca cgtcctccgg ctgctggcgg acgccgagct
      181 cggcacccgt ctcgcagccg cggcgcgcaa cctcgtccgc gagcggcata cgtgggaaag
      241 gagcggcgaa cggctgcacg aggccgtcgc cgaggcggtg tcggcatgga gagtggcagg
      301 acgatgaacc acgcgcaagg acgcgtcggc acggcggcca ggttcgcgct cgtcagcgcg
      361 gccgtgatcc tcgtgaccgg gctcccgtac ctgtacgggt tcgcggccgc agggccagac
      421 cgcgtgttca cggggctgat gtacgacgtg ccggaccacg cgcagtactg gtcgtgggtc
      481 accgcctcgc gcgagagcct cttcatcagc aacacgatga cgcccgaggc gaatccgccg
      541 atcttcacca acccgatgat gtgggctctc gcccaggtgc agcgggcttt cgggctctcg
      601 ttcccggcgc tgttccaggt ctggcgcttc gccgcggtct tctcgctggt ggcggcgctc
      661 tggtggtttg tcagcgtcat ggtccccgag cgcgcccggc ggagaaccgc cttctggctc
      721 tcgctggcag gctcgggcct gggctgggcc ctcatcgtcg ggaaagtcgc gctgcgactg
      781 ccggacgccc cctatccggt tgacatctac acgatcgagc cgaacacctt ctggggcctg
      841 ctggcctatc cctacctgcc cctggcccag tcgctactgc tcgcgagcgt cgtctgtgtg
      901 tggcgggcgc acaagcgccc gagcgtgccg gccttctttc tggccggcct ggcgtcgctg
      961 gccctggccc tcgtgcatgc gtacgacctg atcgtcctgt acgccgtgat cggagcgttt
     1021 ggcctcgccc tgctcgcgcg tgaccggagg ctcccgctgg ctctcgcagg cgccggcctg
     1081 acggtggcag cgtgctcggc gccgatggcg tggtacttcc agtcgctgac gtccagcaat
     1141 ccgctctgga gatcgattct cgcccagtac tcgggcgccg gcgtgtggac gccgcgccat
     1201 gtccacctga tcgtcctgat gggactgccc ctgatcctgg cggttccggg cttctttcgc
     1261 cggggcccgc ggaccgacga gcggcttctg gtgtcggcct gggcggccgt cgggctggtg
     1321 ctgatctacc tgcccgtcgt gttccagatc aagatgctcg ggggctggca gttcccgatc
     1381 gcagccctcg cggctggcgc gtggcacgag tccgtcgagc cgtggatctc caggaagttc
     1441 cctcggcgcg tgggcggtgt gagcgccgtg accgccgccc gcgcgctcct cctgctggcc
     1501 gtcctgccga ccaacgtcta cctgtacgcc tggcggttcg tcgagctgcg gcgtcaggcc
     1561 ccgccctact acctgcatcg ggacgaggcg gccgcgctgg actggctgag gcaccacgcc
     1621 gcgccggaag acgtcgtgct cgcgccggag gcgatcggcc agttcgtgcc aaactacgga
     1681 gagtctcgcg cctaccttgc ccactgggcc atgacgaacc gctaccatga gcgcgtggcg
     1741 cgcgtgcggg cgttcttcga cccggacgtg gtcgatgcgt ggcgggccca gctcctgagg
     1801 caggaaggcg tgacgctggt gctgcgggcc ggcagcgtgc cgggcctgcc gcgcctgtac
     1861 gacccaggcg gttcgccctt gtgggacgcg ctgttccaga gcccgcgcgc gcagatctac
     1921 cggctgcggc cggcgccgcc ggtggaggtg gcggagcagg gatcgccggg tggaaggggc
     1981 gttcgatgag cgctgtgaat cagctcggct tctacctccg cagcaagacc aggagcccct
     2041 ggcgctacgc gctcggcgag acgctccagg cgctgctcgc ctgggtcccg ggcatcgtgg
     2101 gcatcggcct ccgcgccatc gcctacaagg tggtgctgaa ggcggacggg cttcctgcca
     2161 tcgaggacca cgtgcgcctc gttcgctgcg aggacatccg catcggccga tccgtgtaca
     2221 tcgaccacgg ggtgtacctt cacggcgggc agggcggcct ggagatcggc gacggttcct
     2281 ggctgatggc cggctgccgc cttcacgtgt tcaacttccg gggcatctcg cacgccggta
     2341 tccggatcgg ccggcggacc ttcctcgggg aggagacgct catgcgcgga cagggcggga
     2401 tcacggttgg cgacaacgtg ctgttcgcgc cgcgcgtcca ggtgctcgcc gtcgatcacg
     2461 tcatggcgga tcccgcaagg cccatcatgg atcagggcat caccgcccgc ggcatcgtca
     2521 tcgacgacgg ctgctggatc ggggcgggcg cggtggtgct cgacggcgtg accatcgggc
     2581 gcggctcctg cgtgggcgcc ggcgccgtcg tgacggcgtc ggttccgccg cacagcctcg
     2641 ccgtgggcgt gccggcgcgc gtcatccgga acctgcgcga caatccgctc gcggctcccg
     2701 atgcggccgt ctacttcggg ggcatggacg aactgcgggc gtaggcgcgt cgggacgccg
     2761 cccggcgcgg gcccacggag ccgccgatgc ggatcctctt cctgaccgag accattccgt
     2821 ttccgctcga cagcggcggg cgggtcaaga cgtaccacac gctccagtcg ctggcggacg
     2881 agcacgagat ccactgccat gccttcatcc ggtcggcagc ggaacggcgc cacgcgggcg
     2941 agctggagcg gcactgcgcg tcgctgaccc tccacctgcg tccgcccggg cccgcgcggg
     3001 aggccggggc gctcgccctg agcctggcga cgcgaacgcc cttcaccgtg gcgcggcact
     3061 acttccgcgg cgtactacga ccacctcagc atgctcgcgt acgcgcggcg cctcgggctg
     3121 cccgccatcc acgacgcgca caacgtcgag tacgacctgg tcaggcgcca cgccgccacg
     3181 ctgccctggt ctcccgtccg gctgttcgtc gagcgggagt ggcgcctgct gagacggtac
     3241 gaacgggcgc ggtacgcgcg atgcggcctc atcttcgccg tgagcgaacg ggacgccgcg
     3301 gcgattcggt cgctcgcagg cggcgcgccc gaggtgcgcg tcgtgccgat ctcgattgac
     3361 gcgcgcgcca tggcggccgc cggccctcgc ccgcgccgcc cggacctgct gtttgtgggc
     3421 ggcctgcact ggccgccgaa cgccgacgcc gtggcccact tcgcccgcga catctggccc
     3481 ctcgtccgac gcgaggcgcc ggatgcgacc ctgaccgtcg tgggccgagc cgacgcgccg
     3541 gtggcccagg ccctccggcg gatcgacggc atccggctcg ccgggcacgt ccccgacgtg
     3601 gcgccctatt tcgagagggc ccgcgccttc gtcgttccgc tccgagccgg gggcggcatg
     3661 agggtcaaga tcctcgaagc gctggcgcgc ggtctgccgg ttgtcagcac cgcgatcggc
     3721 gtcgagggca tcgatgccgc gcatggggtc cacctgctgg tggccgatga cccgggatcg
     3781 ttcgcgcggg cggtgagcca ggtcgtcggc aacgacggcc tggctgcgga actcgccgcc
     3841 gccgggagga ggctggtgct ggagcgttat gatcgctcgg tggtcggaga gaagctgcgc
     3901 cgggacctga gggagtgtgg cggccaattg ggacggccga ccgatgctga acagggcaga
     3961 caaaagtcgg ccatcagggc gagttttgat tgacacggaa ctccccatgc cccgctcccg
     4021 ggcgcgccgt caacccagct ctctgccgca aggcccgccg gcggctgaaa cagccggtga
     4081 gcggcccaca gcgcaatcag gccccgtggg cgcccgcgac gctggcgcca cgctgacacg
     4141 ggcacgagtc ttgctgttcg accagaggaa ctcatccgag tcaggacggg cgcccgcaac
     4201 ctctctcgag gcacgcatgg ccagcacacc agattccgcc gccccaccgg aaccagacat
     4261 ggccggcagt cgaaccgcaa acgggttgtc gatctgtatc ccggtgctca acgaagaggg
     4321 ctccatcggg gagaccctcg agcggtgcct ggcgctcgcc ccggccctcg gcgccatcgg
     4381 cctcgacggc ctcgagatca tcgccgtcga cgacggatcg accgaccgga cggccgaacg
     4441 ggtgcgcgag caccccgggg tgcgcctcat tcagcacccg gtgaatcgag gctacggagc
     4501 ggccttgaag accgggttcg ccgcggcccg ctacgacgtg attgccttcc tcgatgccga
     4561 cgcgacctac ccgccggagg agttcccggc cctctgccaa ccgatcgtcg acaggaaggc
     4621 cgacctcgtg atcggctccc gcatgctggg cgccgactcg gagatgccgc tcgtccggcg
     4681 gatcggcaac gccttctttg cgtcgctcct gagcctcgtc ggccgctcgt cggtcacgga
     4741 cagcgccagc ggcatgcgcg tgttccgccg ggaggccctc ggactgctct cgccgctgcc
     4801 ggacggcctc aacctgacgc cggtcatgag cacgcgggcg gtgcacgagc gcctgaacgt
     4861 cgtcgaggtg ccgatcacct accgcgagcg cgtcgggcgg tcgaagttga gcgtcacgaa
     4921 ggacggcctg cgcttcctga agaccatcgt ctggacgacg ctcacctaca accccgtccg
     4981 gatcttcggc gccgcggggc tggccgggat cggccttgcc gccgtcatcg gcctcgcgct
     5041 gaccgccatg cgcctcagcg gcgtcacctc gctcggcccg atcggcgcct tcagcgtgtt
     5101 cgcggccgtc gtctgcgccg ttgccggcgt gagcgtcttc gcgctcggcg cgtcgttcaa
     5161 ctacctcgtg tcgctcttct acaagcggcc gatccgccag ggcctgttcc gcaagccgct
     5221 gttcgaagtg ccgatcgaac gctggttcct gccgattggc ctggcggcgt ttgccgcagg
     5281 cgcctcgatg tcggtcgcga gcctggcgct cgccgtcggc ggctgggcca tcgaacgcct
     5341 gtggctgtac ctgtccgcgt cgtcgctgct catcctggtc gggctgcacc tgacggcctg
     5401 gtggctcgtc atgtcggtct tgcacgagct gagcgcgggc gggcgcaccg aggaggcctc
     5461 ccgtgggtga gcggcagccg gaagccgcgt ccgaggcgcg gaacgtcggg cgcaagacgc
     5521 gcctgatcgt gctcccgccg gcgcatccga acaccgacgc gattgcgcgg gacagcgcga
     5581 tcgcccgcgg cgaggcgagc aaggtggacg tcctgctggt caaccctccc tcgcccgacg
     5641 gcgcggtctg gatccgctcg cagcaccggg tcggccgccg ctcgcgcgag aacatgctgt
     5701 ggccgcaggt ttcgctcgcc cagatggcgg ccctgctcca gccgacctac tcgatcagga
     5761 tcatcgatgc gattgccgag cggatggact accgggcctt cgaggacatc ctgcgccgcg
     5821 agcgcccgcg ctactacgtg acgcagctca ccggaccgac gctccagtcc gacatgtacg
     5881 gcgcgttcct cgccaaggcg aacggcgcga cgacgatcgc gttcgggacg cacatcacgc
     5941 cgatgccgac cgagacgctc cgcccgtttc ccgcgctgga ctacggcctg cgcggcgagc
     6001 cggacctgac gctctgcgac ctcatcgacc acctcgaggg ccggcagttc gatcgcccgg
     6061 cgctcctcga gcccctcttc cggaagcacg acccgctgta ccagccgagg gcgctcgcga
     6121 cgctcccgga cggaagcccg gacctctccg gcgtgcgggg gctcgtctgg cggcggggcc
     6181 aggagatcgt ggtcaacccg gatcggccgt tcatcgccga cctggacgac cttccgatgc
     6241 cgctgcatca cctgctgccg ttcaaccgct acctgatgcc gctgctcaag gggccgttca
     6301 cgttcatcgt cacgagccgt gggtgccccg ccggatgcac gttctgcatc aagcacgtga
     6361 gctacgggcc caccatgcgg atgcggtcgc cctcgaaact ggtcgaggaa ctgcaggtgc
     6421 tcgacacgct cggcgtgcac aacatccata tgtacgccga cctgttcacc gtcaaccgcg
     6481 accaggtcgt cgggctgtgc cgcggcatcc tcgaggcggg cctgcggatt cgctggacct
     6541 gcaacagccg ggtggatttc gtcgaccgcg agatgctcca gctgatggct cgatcggggt
     6601 gccatttcat ctcgtggggg ctggagtccg gcaacgcgaa gatcctggcc cacgcgcgga
     6661 agggcgtcga tcccgaccgc gcgcgccagt cgctgacgct cgcccacgaa gcgggcatca
     6721 ggaactgggg ctacttcatc atcggcctgc cgggcgagac cgaagagacg atccgccaga
     6781 ccatcgcgtt ctccaagagc cttccgctgg acatcgccct cttccacatc gcggcgccgc
     6841 atccgggcac cccgttcttc cacgaggtcg tcaggaacgg gtggttccgc aagggcgtcc
     6901 ggtgggagga ggtcgacatg gacgagtcga cggtgctgga ctacccgggc cttccggccg
     6961 agcggctgga gtactggcag aaacgggcgt tccgcgaatg ggccatgcgt cctggtccga
     7021 tgctgacgta cctcaggatg gtgctgtccg acacgcggac ggtcaagtcc gccctgagcg
     7081 tcgggctgca gcacctgggc tgggcgaagc ggtgatcggg cgccgcgccc ctgcccttgc
     7141 gcccaccgac cttcccgtcc tgttcctgct ctgcagtgct ctcgcggccg tctggccggc
     7201 cttcgaccgc gcctcgggct ggcgcggcgc cgcggcggtg gccgccgggg ccctggtgta
     7261 tgccgccgcg tcgcgcctga gggcggcggg gccggcctgg cgggccctcg gcggagcgag
     7321 cgtcgtggcc ggcgcggcgc ttggagcgta cttcatcttc cagttcccgc gcctcggcta
     7381 cgcagagaag tccgccgtcg tcgagcgcgc ggcggcatgg ctgggctcgg gtgcgcccgc
     7441 gttcggccac tgggcgccgg atcgcaattc tgtcgcgacc tgcctcgaag gcctcgtgtt
     7501 cctcggtgtg gcgctggcct cgacgcgcaa aggccggctc gcgcgtgtcc tctggggtgc
     7561 ggcggtcgcg atcgcggcgg ccgcgatcgc gctctcggcg tcccgcggcg cagcgctcgc
     7621 cgttgccgcc gccgggacgg tctgggccgc catgcgcctg aagcccgtcg cggctcggcc
     7681 cgcgctggct agcttcctgg tcgcgctgcc gctcctctcc ggcctggtgt ggcttgccgc
     7741 tgtcgtgtgg gagccgggcg cggcggcagg cctcgagacg gtgtcgccgg cgctggacag
     7801 gccggaccgc gtcgcgctct atcagcagag cgcctggctc atccgggacg ctcccttcac
     7861 ggggatcggg atcggcagcc agttcgccat gagcctgtcg cgctacgcgc tcctcatcca
     7921 ggtgccgttc ctcacctact cgcacaacct ctacctggag acctggctcg aactcgggct
     7981 cgccggcgcc gccgcgctcg tctggctggt cgccgcgtac gccgccggat tcaaggcaat
     8041 cccccagatc aggcatggcg cgctcgcgca gggcgccggg tgcggggtgc tcgccgtgct
     8101 gctccacgga ctggtcgacg cgaggccctt cgtcgattcg tggagcggtc ttccggcatt
     8161 cgtgctgctc ggcctcttcg cgtcagccgt ctcggcggcg gagacccctc cgatgcggcg
     8221 gcggcggcgc cgggtttcgg ccgcagccgt ctgcgggttt atcctgtgcg cgctgatcgc
     8281 cgtccggccg gcggcgtcgt cgtggcacgc caacctgggt gcggtggcgc aggcgacggc
     8341 cgagttgagc gcggacgcct cggacgcgga acgccggacc gccctgcagg aggccgaacg
     8401 ccgtttccat caggcgctgg gcggcgtccc ggagcaggtc acggcgaacc tgaggctcgc
     8461 actcatcgac atggacgacg gccgcttcgc cctggccgtg acgcgggccg agacggcctg
     8521 gcgcggcgcg ccgaacaccc tgcgcacgcg aaaggccttc ggcctcgcca gcatgtggac
     8581 tggggacctt gaccgcgcgc gcgtcctgct cgaatccgtg cccggcatcg tcgccgaact
     8641 caacgcgtgg ggctggtgga ggcaggaccg acaggagccg tcgcttgccc tgcgcgccta
     8701 ccggacctcc ctgctcctcg accccggcca gaccgacgtg cgccgggccg cggagtccct
     8761 ggcccgggcc gcctcgggcg acaggtgacc ggcgtcgccg gctggcggga gccagccggc
     8821 cgctcggcat cgaacggccc gggcgctagc cctgacggta gtacctctcg tactccggat
     8881 tgtagtagcg cgcgtagtag tagcggttcc gcctgagctg gacccggttc aggacggccc
     8941 cgaggatctt cccgtccgac gagtccagct cgcccagcgc ggcctgcgcc gcgccgcgcg
     9001 ccgtggcatc ggcccccacg acgaacacca cgcccgacac ggcatcggcg atcagcgtgg
     9061 cgtcggtcac ggccatgacg ggcgggcagt cgatgatgac gtagtcatag tccgagccgg
     9121 cgcgctcgat caactggcgg aagagcggaa ggatcaggag ttcggccggg ttgggcggcg
     9181 cctccccgga tgggaggacg tcgaggccgc gctcgacgcc gcggcagatg ctctggcccg
     9241 ggtgcgcccg cgacgccagc acctccgaca ggccaggctg ctgtctgagc ccgaagatct
     9301 cgtgaacctg ggggcgccgc atgtccgcgt cgacgagcag gacgtgccga tcggcctggg
     9361 cgagcaccac cgcgaggttg cacgccatgg ccgtcttccc ttcctgcggg ctcgtgctcg
     9421 tgatggcgat cgacgtggcg ccccgcttgg cggcggcaag catgaggccc gtgcgaagac
     9481 ggcgcatcga ctcgttgaag tcggccgacg atcgcttgcc gaccagcggc atctcgtggt
     9541 cgagaacctt cgactcgacc gcggggatga ggccgagaga cggcagatcg aggtaggcgc
     9601 ggatttcctc cggggacttc agcctggtgt ccgcgagttc ggccccgaac acgaggccga
     9661 gcgccagcac ggccgcgccg agcaggctca ggaacaggct cctgccgcgc tggggcctca
     9721 ccggagccgt cggccggacg gccgcttcga caacacggag gttgctgctg ctcacagcgg
     9781 ccacgagccc ggtttgcttc gcctgctgca gcagggagtc cagcacctgc cggttggaag
     9841 cggcatcccg ctccaggctg ccgtactcga tcccgacgcg gcccaggtcg agcgcctggc
     9901 gcttctgggc ctcgagcgcg gcggtcaggc ttcgctcctg cgcctgcgcg gccaggtagt
     9961 cgttccggac ggcctcgacc gtctttgcaa tctcgctttg cagccggctt tcggccgcct
    10021 gcagcgccat gcgcacgcgg accatctcgg gataccgctc gccgtagcgc tccgccagct
    10081 ggctgtgctc cttctgcaag tcggcgacct gggccttcag ctgctggatg taggggttcg
    10141 acaagatcag aggaaaggtg tcaagggccg cctggctgcc ctgaagggcc gcgagctggg
    10201 tgtacaggcc ctcttttgcg aggcgatcgg tcttggcgcg cgtcaaggcg gcgttcaggt
    10261 ccgccagctt ctgcacgacg atgttctggc ggtcgtcgag agcgaccgct ccgtgctctt
    10321 cccggtactt ctggagcgcc gcctcgctcg cgtccactgc agcccgctgc tccgagaggc
    10381 gcttttccag ccactcggag gcgtgccgag ccgactggta tcgggactcc aggtcctggt
    10441 cgatggcgag ccgcgccagc gcgttggcga ccttcgcggc cagggccggg tcctgcgact
    10501 ggaacacgat gtccacgagg cggctgttct cgatcggcct gacggacaga cgcgcgagca
    10561 gcgcatcgac gaggggcgac gcctggtccg cgggcgcagc ctgggcggcc gccggagggg
    10621 ccgagccgcc gaggagacgc cgcacggcgg tctgggcccg ccggaacgcc c
//
LOCUS       DAOSIF010000045        10600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6134_length_10600_cov_3.523661, whole genome shotgun sequence.
ACCESSION   DAOSIF010000045 DAOSIF010000000
VERSION     DAOSIF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6134_length_10600_cov_3.523661"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..970)
                     /locus_tag="PLT35_02840"
     CDS             complement(398..970)
                     /locus_tag="PLT35_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOG28190.1"
                     /translation="MTFQVGDKVIYPNHGLGVVERIEEKTILGTTCGFYHLRIAANDT
                     TVLVPLANVDGVGLRRAIDEDEVDRLFKLLGDGKIDSQQNWKGRFKDNSEKMRTGSIY
                     DVADVLKSLTLLSKSKSLSFREKRMLDRARFLIISEIAEVTSRVSSAVEGTLDQALAK
                     CIAARARSIERAKTAKAPGKAASAKRAKAS"
     gene            1305..1393
                     /locus_tag="PLT35_02845"
     tRNA            1305..1393
                     /locus_tag="PLT35_02845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1339..1341,aa:Leu,seq:caa)
     gene            complement(1526..3817)
                     /locus_tag="PLT35_02850"
     CDS             complement(1526..3817)
                     /locus_tag="PLT35_02850"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOG28191.1"
                     /translation="MTRARVGFAALPLLALAAMPPPAAAQPVPDRPVRPTIVIPRVDQ
                     APALEDFLDMQPGPRMAGRMLKLTGFTQTLPRDGEPVSQATDAYLAYDSERIYVVIVA
                     FDAQPELIRARMTPREKFSGDDKIDVFLDTFHDERRAYGFTCNPLGVQMDGRWTEGSN
                     FDSSFNAIWQSKGRITDRGFIIWMAIPFRSLRFPAVAQQKWGLVIIRWIPRNNENSAW
                     PWVKGTIDGRLNQGASMVGIEGIAPGRDVQLVPYGFVRSFRALDTQDPERVAFAGRSA
                     DPDVGLDAKFVIANRFALDAAVNPDFSQIESDEPQITVNRRFEVFFRERRPFFLENTD
                     FFSTPINLFFSRRITEPRLGLRLTGKEGPYTIGAIVADDEAPGKAVPPGDRLRGSRAK
                     LGVVRVNRDLFRNTTAGFLVTDRELAGGRNRVGSVDSRFKLTPNWVGTLQAATSSTRS
                     IDGAEVAGQAYAAALRREGRHFTQSTEYLDFSPGFQTQTGFVSRTGIRSAAQQVNYLF
                     RPQGRSLLSWGPSFGASGVWAHDGGAPLDWELQARLNVEFTRQTKLGVFYRPGRERLR
                     PKDFAGLAASADFSTGLRGFSLSSQHISALTFSSTYTAGRVINLVPPRGAGPAPADSA
                     EVTVAVTARPNRHLLSENTYIMSRLTSVGAGERIFSNHIVRSRWNWQFSPELSLRVIL
                     QYDAVFASPPRTRLETRRNLNADVLFTYLLNPWTALHVGYNGNRQNIALQPTDAGRRV
                     VRAGGMFEDARQVFVKVSYLVPL"
     gene            complement(4155..5498)
                     /locus_tag="PLT35_02855"
     CDS             complement(4155..5498)
                     /locus_tag="PLT35_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG28192.1"
                     /translation="MTDRHTPINADVVTRALEGAGLLSLSTASIREVKRLIDTIEGET
                     GQRFIRMEMGVPGLPPNQAAVRAQIEALERGVAARYPDIQGIPPLKLQISRFVKNFLD
                     VSVDPEHCLPTVGTMMGAMACFLTLNRMHADREGTLFIDPGFPVQKQQCRVLGHGFMS
                     FDVYDFRGPRLEDKLRSYFDSGKVSSVLYSNPNNPAWICLTDAELEIVGRLATEYDAV
                     VIEDLAYIGMDFRRDYSHPGAPPFQPSVARYTDNFVLLISSSKVFSYAGERIGMLAVS
                     DAIWDLRAPDLKRYYASDSLGHALVFGAIYALSAGTSHSAQYALAAALKAVNDGEANL
                     LEDVREYGEKARVMKRMFTDSGFRIVYDTDLDAPIADGFYFTVSYPGLSGEELLRELM
                     YYGISAIALSTTGSARTEGLRACTSLIQRSEFPLLAERLAWFRRDHPVEPGAAAR"
     gene            complement(5544..6998)
                     /locus_tag="PLT35_02860"
     CDS             complement(5544..6998)
                     /locus_tag="PLT35_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG28193.1"
                     /translation="MPQHARRPAPRRLTRREFVGGALTAAGAVAGAPAILRGRNLNDK
                     LDIAFIACGGRAVASLRELTLAYGPGGDSRGAASPPLAPHPDENVVALCDVDQNALDE
                     ASQRFPKARTFTDLRRVFDHPGAFDAVVVSTAEHTHAFATYLALTHGKHVFCEKPLTY
                     NIWEARLIRETAAKHPRLSTQMGNQGHASPSRRKIKEILMTGVIGPVREVHVWADRAW
                     GLQDAASADKFDRPHGFYNGVQIVDRFAEEMPVPATLHFDLWLGPAPVRPYHATYFPG
                     PRWYRWWDFGNGTMSDLGSHDNDVPYTVLDIRQPLTVEAESPNVPVAHRELAPATMKV
                     TYEFPASPSGAPPVKLVWYQGDCKPPGWTPEWGGRSQIFIGDHGMLLGSGKLLPEEKF
                     KDFQAPPETLPRSPGHWVEWVNFAKGRGPAPGSNFQYSGWTTEANHLGNVAYRVGKKI
                     EWDHRSLRARNAPEAAPFIARPEYRKGWDDILKP"
     gene            complement(7132..8496)
                     /locus_tag="PLT35_02865"
     CDS             complement(7132..8496)
                     /locus_tag="PLT35_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG28194.1"
                     /translation="MGQQVMGAAMQQDQTAWTRREFCRVGAMGIVGAALADPRAALAA
                     PAKTRVAIVGTGIRGSTTWGMNLVRDAGEFVDIVGLCDVNPDRVEVARRWLGGAVPAF
                     TGFDEMVRTVRPDRVIVTTVDATHADYVCRTMDFGLDPISEKPLCTQAEQAQRIVDTQ
                     KRTGRRLDVTFNARHESSAMKVKELLLAGEIGDLYSVNYEEFLDLDHGASYFRRWHGL
                     KQASGTLLCHKASHHFDQLNWWIDSDPVEVTARGRLNRYGVNGPQRHANCRLCPHKKA
                     CDLYWDITSSKQMMELYVDCEDQDGYFRDGCLYRREINIPDTYSVQVTYANHVLVTYS
                     LNAAAPYEGQAIVFNGSKGRIDVRNYNRQPWQVPHESEIRLTRSFKDSVVMAADPQRG
                     ESFEHGGADQRIKRMIFDPGVADPLAQRAGLKAGIMSSAIGIAGYTSIDTGRTVRIEE
                     VVRL"
     gene            complement(8481..9404)
                     /locus_tag="PLT35_02870"
     CDS             complement(8481..9404)
                     /locus_tag="PLT35_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOG28195.1"
                     /translation="MTTTRLFRARLGAPRQHLGFPAAILLLGGPALFALACGPGASAP
                     AERPPLKIYVNTDLEGVSGVFKFAQTREKDSPLNIQACEYFMGDVAAVVRGLRDAGAT
                     EVVVLDGHGTQAVLPHLMAPGAVYVTGTPRPGAGNLSGLDESFAGMVMLGFHAMMGTP
                     DGVLNHTQSSRTENRYWYNGVESGELVQNAAIAGHYGVPLVMVTGDEATCREARQFFG
                     DGVVTVATKRGLSREAAVLYPFEETRKALYDGARRAVEATRVCKPYVLKAPIAVREEY
                     LNLDPSLPAPVKVVREGVAENALQLFTWANK"
     gene            9525..>10600
                     /locus_tag="PLT35_02875"
     CDS             9525..>10600
                     /locus_tag="PLT35_02875"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOG28196.1"
                     /translation="MNPFIRRILFACLVSGAALGTVPPVRTAPPSHAGPPPAAAAMAV
                     TGLRCEYAVSPMGIDVPRPRLYWRVQSPERGTRQTAWRVLVSSSPEFLAADRGDLWDS
                     GRVASDESAHIPYAGSPLASSQRVYWKVRAWDQAGRPSAWSLPATWTMGLLRAEDWKG
                     AWIAAPGETETLLLRGTFDVRPGLVRAVAHASGLGHYELTLNGRRVGEDLFAPGWTNY
                     ARTVLYDTHDVTALLTTGRNAAGFLLGNGMYHVVRRNRFAKFTGSFGPLRAVLHLRLE
                     YADGSVEYAGTNAGWRSHPGPITYSSVYGGEDYDARLEPRGWNTAAFDDRGWGPVVPV
                     IGLEHAMRGLSAAADPIRPIAERP"
BASE COUNT         1512 a         3716 c         3659 g         1713 t
ORIGIN      
        1 catccggcac ctcgacaagt cgtacgggcg cctcgccgtc ctcgagcacc tcgaagtccg
       61 aggcctccag gtcctcgacc ggcgcgccgt ccttcgaggc gaacacgtcc acgcggacga
      121 agttcgcgcc ggcgcggaac accgggcgct gctcccggcc agccgcgtct gccgcccggg
      181 cccctgcgcc ccccgggggc ggcccctggg ccccgcgggc gtccaggccg ccgaggccag
      241 ccaggaccgc tgtcagacaa aggccggcgg ctgtcgcgaa cgagcagcca gacgggcggc
      301 cggagcgggc gggcgcatcc acgtccgcat cataatcgaa cgggcccgcc ccggcttggc
      361 cgggacgggc ccgtgagacg cgaaccgagt cgccggacta ggacgccttg gcgcgcttgg
      421 cggaagccgc cttccccggc gccttggccg tcttggcccg ctcgatgctc cgcgcgcgcg
      481 cggcgatgca cttcgccagc gcctggtcga gcgtcccttc gaccgccgaa ctgacgcggc
      541 tcgtcacttc ggcgatctcg ctgatgatga gaaaccgggc cctgtcgagc atccgcttct
      601 cgcggaagga caggctcttc gacttgctca gcagcgtcag gctcttgagc acgtcggcca
      661 cgtcgtagat cgagcccgtt cgcatcttct cggagttgtc cttgaaacgt cccttccagt
      721 tctgctggct gtcgatcttt ccatccccaa gcagcttgaa gagccgatcc acctcgtctt
      781 catcgatcgc gcggcgcagg ccgacgccgt ccacgttcgc gagcggaaca aggaccgtgg
      841 tgtcgttggc ggcgatccgg aggtgataga acccgcaggt ggtgcccagg atggtcttct
      901 cttcgatgcg ttcgacgacg ccaagtccgt ggttcgggta gataaccttg tcgccgacct
      961 ggaatgtcac tgaggtcccc tctctttctg caggaatgag tcgaaaggtc ggagggccgg
     1021 aggcacctgc aactcttgca gtatacgccc gggactgcct cggatcaacc gaaaaccttc
     1081 gtgtttcggc gaggtttcgc ctgtctttcg ctgattcgac ttgttttcgc cactggcgcg
     1141 tcgcccggat tcgcaccgga ctcgcgcctc cggatcgtgc caacgtgctg atggtttgga
     1201 cgttcgcggc tggtgcgagg gttcaggcgc cggggagcgt cggacggcag ggggcggctg
     1261 cggatgctat actcgggggt ccgccgcccc ttgccttcga acgtgccgga gtggcggaac
     1321 tggcagacgc gcgggactca aaatcccgtt gggttcaccc ccagtgtggg ttcgattccc
     1381 acctccggca ccaccttgtc aacaagttac ggccctcgca attccagcgg ctcctgaccc
     1441 gagaggcccc tggctgacgg atggctgacg ggacgggaga tcccgcgggg ctctcccagg
     1501 cgctgaagaa ggcgggtctg ccccttcaca gcggtacgag gtaggagacc ttcacgaaaa
     1561 cctggcgggc gtcctcgaac atgccgcctg cgcgaacgac ccgccgcccc gcatcggtcg
     1621 gctggagcgc gatgttctgc cgattgccgt tgtagccgac gtggagcgcg gtccacgggt
     1681 tcagcaggta ggtgaagagc acgtcggcgt tcaggttcct cctggtctcc agcctggtgc
     1741 gcggcgggct cgcgaacacg gcgtcgtact gcaggatcac gcgcagcgac agctcggggc
     1801 tgaactgcca gttccatctg gatcgcacga tgtgattgct gaagatgcgc tcgccggcgc
     1861 caacgctggt gagcctcgac atgatgtacg tgttttcgct cagcaggtgc cggttcggcc
     1921 gggcggtgac ggccacggtg acttcggcgg aatcggccgg cgcaggcccg gcgcctctcg
     1981 gggggacgag gttgatgacg cgccccgcgg tgtaggtgct gctgaaggtc agcgccgaga
     2041 tgtgctgcga gctgagcgag aagccccgga ggccggtcga gaagtccgcg ctcgccgcca
     2101 gaccggcgaa atccttgggc cgaagccgtt cccgtccggg cctgtagaac acgccaagct
     2161 tggtctgccg cgtgaactcg acgttcagcc gggcctgcag ctcccagtcg agcggcgccc
     2221 cgccgtcgtg ggcccagacg ccgctggccc cgaacgaagg cccccaggag agcagcgacc
     2281 ggccctgagg ccggaagaga taattgacct gctgagcggc gctgcggatg ccggtcctgc
     2341 tgacgaaccc ggtctgggtc tggaagcccg ggctgaagtc gaggtactcg gtgctctggg
     2401 tgaaatggcg cccttcccgc cggagcgccg ccgcatacgc ctgccccgcg acctccgctc
     2461 cgtcgattga gcgggtcgaa ctcgtggccg cctgcagcgt gcccacccag ttcggcgtca
     2521 gcttgaaccg ggagtccacg ctgcccacgc ggttgcggcc ccccgccagt tcgcggtcgg
     2581 tgacgaggaa cccggcggtc gtgttccgga agaggtcccg gttgacccgc acgacgccga
     2641 gcttcgcccg ggacccgcga agccgatcgc cgggcggcac ggccttcccc ggggcctcgt
     2701 cgtcggcgac gatggccccg atggtgtagg gcccttcctt gccggtgagc cgcagtccga
     2761 ggcgcggctc ggtgatgcgt cggctgaaga acaggttgat cggcgtcgag aagaagtccg
     2821 tgttctccag gaagaacggc cgccgctccc tgaagaaaac ctcgaagcgg cggttcacgg
     2881 tgatctgcgg ctcgtcggat tcgatctggc tgaagtcggg gttcaccgcc gcgtcgaggg
     2941 cgaagcggtt ggcgatgacg aacttggcgt cgagccccac gtccggatcg gccgacctgc
     3001 cggcaaaggc gacgcgctcg gggtcctgcg tgtccagcgc ccggaaggag cggacgaagc
     3061 cgtacggcac gagctggacg tcccgccccg gcgcgatgcc ctcgatgccc accatgctcg
     3121 cgccctggtt gaggcgcccg tcgatggtgc ccttcaccca gggccacgcg gagttctcgt
     3181 tgttgcgcgg aatccagcgg atgatgacga gcccccactt ctgctgcgcc accgcaggaa
     3241 agcggaggct cctgaacgga atggccatcc agatgatgaa cccgcgatcg gtgatccgcc
     3301 ccttcgactg ccagatcgcg ttgaacgacg agtcgaagtt gctgccctcc gtccaccggc
     3361 cgtccatctg cacgccgagg gggttgcagg tgaagccgta cgcgcgccgc tcgtcgtgga
     3421 aggtgtcgag gaagacgtcg atcttgtcgt cgccgctgaa cttctccctc ggggtcatcc
     3481 gggcccggat gagctcgggc tgggcgtcga aggccacgat gacgacgtag atgcgctcgc
     3541 tgtcgtacgc caggtaggcg tcggtggcct ggctgacggg ctcgccgtcc ctgggcagcg
     3601 tctgcgtgaa accggtcagc ttcagcatgc ggcccgccat cctcggaccg ggctgcatgt
     3661 cgaggaagtc ctcgagggcg ggcgcctggt ccaccctcgg gatgacgatc gtcgggcgga
     3721 cggggcggtc gggcacaggc tgagccgcgg caggcggcgg catcgcggcc agcgcaagga
     3781 gcgggagcgc ggcgaatccg actcgggcgc gtgtcacctc atcgaccctc ggggcgcgga
     3841 ctatcgtgcg cgtcttcgcg gcgtctttcg gcgttgcctc gcgcggcccc tcgactccgt
     3901 cgaaaccggc gtccttcagc cgcctcaggg cgtcggccgg acggagaatg agggacttct
     3961 ccagcctcat cgcgaagggg agcggcgctg gcgccgccgg gagctgcgcg cctcccccgg
     4021 agcgagtgcc tgggcggcag ccggcccacc ggggccgggc ccctccgcga tgcccgccgg
     4081 cgtcctggtg cactcgcgac gaacgaccgg cggcgtcact cgccttcctg gccgccgagg
     4141 aacgtccgcc gcgatcagcg ggcggccgcg ccgggctcca ccgggtggtc ccggcggaac
     4201 cacgcgagtc gctcggccag cagcgggaac tccgatcgct gaatgagcga cgtgcacgcg
     4261 cgcagcccct cggtccgcgc gcttcccgtc gtgctcagcg cgatcgcgct gatcccgtag
     4321 tacatgagct cccgcaagag ctcctcgccg ctcaggcccg ggtacgacac cgtgaagtag
     4381 aaaccgtcgg cgatgggcgc gtccagatcg gtgtcgtaga cgatgcggaa gccgctgtcg
     4441 gtgaacatcc gcttcatgac cctcgccttc tcgccgtact cccgcacgtc ctccagcagg
     4501 ttggcttcgc cgtcgttcac cgccttcagc gccgccgcca gggcgtactg ggcggagtgc
     4561 gacgtgccgg ccgacaaagc gtagatcgcg ccgaacacga gcgcgtgccc cagggaatcg
     4621 gaggcgtagt accgcttcag atcgggggcg cgaaggtccc agatcgcgtc tgacacggcc
     4681 agcatcccga tgcgctcccc ggcgtagctg aacaccttgg agctggagat gagcagcacg
     4741 aaattgtccg tgtagcgcgc gacggaaggc tggaacggcg gcgcgcccgg gtgcgagtag
     4801 tcgcgccgga agtccatgcc gatgtaggcc aggtcctcga tgaccaccgc gtcgtactcg
     4861 gtcgcgagcc gccccacgat ctcgagctcg gcgtccgtga ggcagatcca cgcggggttg
     4921 ttcgggttgg agtacaggac cgacgagact ttgccgctgt cgaagtagct ccgaagcttg
     4981 tcctccagcc gcgggccgcg gaagtcgtac acgtcgaagc tcatgaagcc gtggcccagc
     5041 acccggcact gctgcttctg caccgggaag cccggatcga tgaacagcgt gccctcgcga
     5101 tcggcgtgca tgcggttgag cgtgaggaaa cacgccatgg cgcccatcat cgtccccacc
     5161 gtcgggagac agtgctcggg gtccacgctg acgtcgagga agttcttcac gaagcgggag
     5221 atctggagct tgagcggcgg gatgccctgg atgtcgggat acctggccgc cacgccgcgc
     5281 tcgagcgcct cgatctgggc gcggaccgcg gcctggttgg gcggcaggcc ggggacgccc
     5341 atctccatcc ggatgaaccg ctgccccgtc tccccttcga tagtgtcgat gagcctcttg
     5401 acttcgcgga tcgacgccgt cgagaggctc agcaggccgg ctccctcgag ggcgcgcgtg
     5461 acgacgtcgg cgttgatcgg cgtatggcgg tccgtcaaga cacccctccc ccgggggcgg
     5521 gtccgtcacc cggccatcct accctatggc ttcaggatgt cgtcccatcc cttccggtac
     5581 tcgggccgcg cgatgaacgg cgccgcttcg ggcgcattgc gggcgcgcag gctccggtgg
     5641 tcccactcga tcttcttgcc gacccggtag gcgacgttcc cgaggtggtt cgcctcggtc
     5701 gtccatcccg agtactggaa gttcgagccg ggcgcgggac cgcgcccctt cgcgaagttg
     5761 acccactcga cccagtgccc cggcgagcgc ggcagcgtct ccggcggcgc ctggaaatcc
     5821 ttgaacttct cttccggcag gagcttcccg ctgccgagca gcatgccgtg gtcgccgatg
     5881 aagatctgcg agcggccgcc ccactcgggc gtccacccgg gcggcttgca gtcgccctga
     5941 taccacacca gcttcaccgg cggcgcgccc gacggcgatg ccgggaactc gtacgtcacc
     6001 ttcatcgtcg cgggcgcgag ctccctgtgg gccaccggca cgttcggcga ctccgcttcg
     6061 accgtcagcg gctgcctgat gtcgagcacg gtgtacggca cgtcgttgtc gtggctcccg
     6121 aggtcgctca tcgtgccgtt gccgaagtcc caccagcggt accaccgcgg gccagggaag
     6181 taggtcgcgt ggtaaggccg caccggggcg ggccccagcc acaggtcgaa gtgcagcgtc
     6241 gccgggacgg gcatctcctc cgcgaatcgg tccacgatct ggacgccgtt gtagaagccg
     6301 tgcggcctgt cgaacttgtc cgccgacgcc gcgtcctgca gtccccacgc ccggtcggcc
     6361 cagacgtgca cctctcgcac cggcccgatc acgccggtca tcaggatctc cttgatcttg
     6421 cggcgggacg gggacgcgtg gccctggttg cccatctgcg tcgagagcct cgggtgcttc
     6481 gcggccgttt cgcggatgag ccgcgcctcc cagatgttgt acgtgagcgg cttctcgcag
     6541 aagacgtgct tgccgtgcgt gagcgccagg tacgtggcga aggcgtgcgt gtgctcggcc
     6601 gtcgagacca cgaccgcgtc gaaggcgccc gggtggtcga acacgcggcg caggtcggtg
     6661 aacgtcctcg ccttgggaaa gcgctgcgag gcttcatcga gggcgttctg gtccacgtcg
     6721 cacagcgcca cgacgttctc gtccggatgc ggcgccagcg gcggcgaagc cgcgccgcgg
     6781 ctgtcgccgc cggggccgta ggccagggtg agctccctga ggctggccac ggcgcggccc
     6841 ccgcaggcga tgaatgcgat gtccagcttg tcgttcaggt tccggcccct gaggatcgcg
     6901 ggagcgccgg cgacggcgcc agccgccgtc agggcgccgc cgacgaactc gcggcgcgtc
     6961 aggcgccgcg gcgccggcct gcgtgcgtgc tgcggcatgt tgctgctcct tgctgcccgg
     7021 ccgccccggg gccatcacga ggccatcacg gagcgcctgc tggcgatacc atgtgcccgt
     7081 ctccgccgcg tggtcagcgg ttgccagggc ctgcgggcgg cccggcccgc cctacagcct
     7141 gaccacctcc tcgatgcgca cggtgcgccc ggtgtcgatg ctcgtgtagc cggcaatgcc
     7201 gatcgccgaa ctcatgatgc cggccttcag tccggcccgc tgcgccagcg ggtccgccac
     7261 gcccgggtcg aagatcatcc gcttgatccg ctggtcggcg ccgccgtgct cgaacgactc
     7321 gccccgctgc gggtcggcgg ccatcacgac gctgtccttg aagctccggg taagcctgat
     7381 ctcgctctcg tgcggcacct gccatggctg ccggttgtag ttgcggacgt cgatccgccc
     7441 cttcgacccg ttgaagacga tggcctgccc ctcgtagggc gcggcggcgt tcaggctgta
     7501 ggtcaccagc acgtgattcg cgtaggtcac ctgcacgctg taggtgtcgg ggatgttgat
     7561 ctctcgccgg tagaggcacc cgtcgcggaa gtacccgtcc tggtcctcgc agtccacgta
     7621 cagctccatc atctgtttgc tggacgtgat gtcccagtac agatcgcagg ccttcttgtg
     7681 aggacacagg cggcagttgg cgtgccgctg cgggccgttg acgccgtacc tgttcaagcg
     7741 gccgcgggcc gtcacctcga cgggatccga gtcgatccac cagttcagct ggtcgaagtg
     7801 gtggctggcc ttgtggcaga gcagcgtccc gctggcctgc ttcaggccgt gccagcgccg
     7861 gaagtagctc gcgccgtgat cgaggtcgag gaactcctcg tagttcaccg agtacagatc
     7921 gccaatctcc ccggcgagca gcagctcctt caccttcatg gcggacgatt cgtgccgggc
     7981 gttgaaggtg acgtccagcc tgcggccggt ccgcttctgc gtgtccacga tccgctgagc
     8041 ctgctcggcc tgcgtgcaca ggggtttctc cgaaatcggg tccagcccga agtccatcgt
     8101 ccggcacacg tagtccgcgt gggtggcgtc cacggtggtg acgatgacgc ggtccggcct
     8161 caccgtgcgg accatctcgt cgaagccggt gaaggcgggc acggcgccgc ccagccagcg
     8221 ccgggcgacc tccacgcggt cgggattgac gtcgcacaag ccgacgatgt cgacgaactc
     8281 gcccgcgtcg cgaacgaggt tcatgcccca ggtcgtgctg ccgcggatgc cggtgccgac
     8341 aatcgccacc ctcgtcttcg cgggcgccgc cagggcggcg cgcgggtcgg ccagcgccgc
     8401 gccgacaata cccatcgctc cgacacggca gaactcgcgc cgggtccacg ccgtctggtc
     8461 ctgctgcatc gccgccccca tcacttgttg gcccacgtga acagctgcag cgcgttctcc
     8521 gccacgccct cgcggacgac cttgaccggc gccggcagcg acggatcgag gttcaggtat
     8581 tcctccctga cggcgattgg cgccttcagg acgtatggct tgcacacgcg cgtcgcctcc
     8641 acggcccgtc ttgcgccgtc gtagagggcc ttccgcgtct cctcgaaggg atagagcacg
     8701 gccgcttccc gcgacaggcc ccgcttggtg gccacggtga ccacgccgtc gccgaagaac
     8761 tgcctggcct cgcggcacgt ggcctcgtcg cccgtcacca tgacgagggg cacgccgtag
     8821 tggccggcga tcgccgcgtt ctgcacgagc tcgcccgact ccacgccgtt gtaccagtag
     8881 cggttctcgg tcctggagga ctgcgtgtgg ttgaggacgc cgtcgggcgt gcccatcatg
     8941 gcgtggaagc cgagcatgac catgccggcg aacgactcgt cgagccccga gaggttcccg
     9001 gcgcccggcc tcggcgtgcc ggtgacgtac acggcgcccg gcgccatcag gtgcggcagc
     9061 accgcctgcg tgccgtggcc gtcgagcacg acgacctccg tggcccccgc gtcgcgcagg
     9121 ccgcggacta ccgcggccac gtcgcccatg aagtactcgc acgcctggat gttgagcggc
     9181 gagtccttct cgcgcgtctg ggcgaacttg aagacgccgc tgacgccctc gaggtcggtg
     9241 ttgacgtaga tcttcagcgg cggccgctcg gcgggcgcgg acgcgcccgg gccgcaagcc
     9301 agcgcgaaga gcgcgggtcc gcccagcagg aggatggcgg ccgggaaacc caggtgctgc
     9361 cggggggctc cgagacgggc acggaagagg cgcgtggtgg tcataggcgt ctcccggagg
     9421 tcgcctcgat gctacacgat cgcggcccgg cgcgcacccg gcgccgcctg cgagcgcgtt
     9481 gtacaatccg gggcgcgcat gcgacgcccc cgcgcccgaa tgccatgaac cccttcatcc
     9541 gccgcatcct cttcgcctgc ctcgtctccg gcgccgcgct cgggaccgtg ccgcccgtgc
     9601 ggaccgcgcc gccctcgcac gccgggccgc ctcccgccgc cgcggccatg gccgtgacgg
     9661 gccttcgctg cgagtacgcg gtgagcccga tgggcattga cgtgcccagg ccgcgcctct
     9721 actggcgcgt ccagagcccg gagcgcggga cgaggcagac ggcgtggcgc gtgctggtct
     9781 cgtcgtctcc ggagttcctc gccgccgatc gcggcgacct ctgggacagc gggcgcgtcg
     9841 cgtcggacga gtccgcgcac atcccctacg ccggctcccc gctcgcctcg tcgcagcgcg
     9901 tctattggaa ggtgcgcgcg tgggaccagg ccggccggcc gtcggcgtgg agcctgcccg
     9961 ccacctggac gatgggcctg ctcagggcgg aggactggaa gggcgcgtgg atcgcggcgc
    10021 cgggcgagac ggagaccctc ctgctccgcg gaacgttcga cgtgcggccg ggtctcgtcc
    10081 gggcagtggc gcatgcgagc ggactcggcc actacgaact cacgctcaac ggccggcgcg
    10141 tcggcgagga cctgttcgcc ccgggctgga cgaactacgc ccggacggta ctctacgaca
    10201 cgcacgacgt gaccgcgctc ctgaccaccg ggcgcaatgc cgcgggcttc ctcctcggca
    10261 acgggatgta ccacgtcgtc cgcaggaacc gcttcgccaa gttcaccggg tcgttcgggc
    10321 cgctgcgcgc cgtcctgcac ctgcgcctcg aatacgccga cgggtcggtg gaatacgccg
    10381 gcacgaacgc cggctggcgg tcgcatccgg gcccgattac gtattcgagc gtctatggcg
    10441 gcgaggatta cgacgcccgc ctggagccgc gcgggtggaa cacggccgcc ttcgacgacc
    10501 gcgggtgggg ccccgtcgtc cccgtcatcg gcctggagca cgcgatgcgc ggcctgagcg
    10561 ccgccgccga tccgatccgg ccgatcgccg agcgcccctg
//
LOCUS       DAOSIF010000046        10447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6236_length_10447_cov_4.098826, whole genome shotgun sequence.
ACCESSION   DAOSIF010000046 DAOSIF010000000
VERSION     DAOSIF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6236_length_10447_cov_4.098826"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLT35_02880"
     CDS             complement(<1..790)
                     /locus_tag="PLT35_02880"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOG28197.1"
                     /translation="MRHTRLVSLVAVTALMSFAASAAAAVSGAAPPKGQTPLLITNAG
                     QGPGAKMARLLLQRTGAVSDFDYNAEPQPADLAAKPYKTLMVVLGSTAKGLGASGITI
                     DDEIARLNAMMAEAKKLKLQIVCVLLEGKTRRGKPGGAGTFHNGLFNLEQKADRLLQM
                     PLANELDVIHQRAHEAAREEAGIFDRNAFGERIALAGHVSALDDIVHAREKFGLNAHD
                     LDAGLERAGNGRDARDQPAAANRDGKAINVRRVFQHFERKRALTR"
     gene            1162..2091
                     /locus_tag="PLT35_02885"
     CDS             1162..2091
                     /locus_tag="PLT35_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28198.1"
                     /translation="MLEIRNLTKSYGDVLALDRFSLDLAKGEVLGLLGPNGAGKTTLI
                     SILAGTLGGFTGTVTFQGQSLFASRQLKNRLGIVPQDMAFYEDLSAMDNLLFWGGLYD
                     VPRAELKRRAAEILELVELAGRAKEPVKKFSGGMKRRLNTAIGLLHKPDLLLLDEPTV
                     GIDVQAKVSILDIIRNVGAAGTAVIFTTHQLAEVETACSRVAIMDKGRILAQGTLDEL
                     VRIVGERTFVEISGEFGAGAFSEAIQDMPAPSVELMSAGDNLALLAVSDTDQIPRVMD
                     RLFGRKLAVTDLTIKSPNLETVFLKLTGRSLRD"
     gene            2095..3345
                     /locus_tag="PLT35_02890"
     CDS             2095..3345
                     /locus_tag="PLT35_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG28199.1"
                     /translation="MRRLLHVFTNDVRRHLRAPLVIVVFMAIPLAMTALIGAVFGPKP
                     GANALPRIAVLVVDRDKSVASKLLLGAFDSARMKEMFEVTVIGESEGRERMKAGRASA
                     MIVVPERFTLDLMEWKPVALTVVKNPAEQFLPDVAEEAVNTLAVVLSGAVRAFGDEVR
                     GIRTMMDLPLEAFPWASLGPELAKAQSKVIAASKYLDPLLIRLKSEEVTTAGSRPAVT
                     RADVFSAVLPGMAIMFLLFIVQTVMRDILAEREDGTLRRMMSAPLRPLELVGARILGG
                     WLMGLAVLLVMAAAGRLLFGATWGPFGYFLLLGATAAFWVAAFFALFHALVANRNQAG
                     AIGAPVILVFSLFGGSMMSPDAMPAAFRPIGLFTPNRWFIDGAALVRDGRFPLAAVAV
                     LGASGLALLALAVPALLRQTRSAS"
     gene            3342..4475
                     /locus_tag="PLT35_02895"
     CDS             3342..4475
                     /locus_tag="PLT35_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG28200.1"
                     /translation="MRRLGRVLHVARNDVWRMVRDRPFFFWTLAFPIFFIVVFGMLFK
                     AGDAAPAVASLTVVNQDEGRWGAYFVDKIKAPNIEITVVKAEPADLTRLIVLPPDFSA
                     RIEARRDQALAFKKRAGASERAAARVETRLFQAIARVLSELVLNGDGDLTTFLDGHPE
                     VADLVRVETRFPEKTVTAVPSGFDHSIPGTTVQFIMMMVMIYGGISVMEDRKKGVLSR
                     LLCSPLSTGELFQAKLLGRWSMGVLQALMLFAVGKAFFGLNLGSAPLALLVLAVFALA
                     VAALSVFAGSLLTREDLIVGVSVLLANMFAGLGGCWWPNEIVPPAVRKLAMISPAYWA
                     MDALHALRFFQGGLRDILPHLGILLTLAVVLAVLAARTFRVRP"
     gene            complement(4491..5033)
                     /locus_tag="PLT35_02900"
     CDS             complement(4491..5033)
                     /locus_tag="PLT35_02900"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOG28201.1"
                     /translation="MGAETARPPIAPETTVGSLLAHYPELEASLLALSPAYGALKNPV
                     LRRTVAKVATLRQVAKVGNVPLPTLINRLRADAGQAPASCVDEADETALERPDWADPA
                     LVARTYDARPVIDAGGHPMPQVMRDWAGLRPGEVYELVTPFLPEPLVALAAEKGYRAW
                     SVREGSDLVRTYFARDETRR"
     gene            complement(5023..6279)
                     /locus_tag="PLT35_02905"
     CDS             complement(5023..6279)
                     /locus_tag="PLT35_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOG28202.1"
                     /translation="MSELIEHARRRKDLLKHMILQIHQGAAPDQVRTRIVRLMGQVPY
                     SDVVEVEQELVNEGVPTEEILRLCDLHGEALKGAIDLRSAPEAPPGHPAHTFAEENKA
                     LLWEVDELSRLYAAADSAPADADASEIVAAIRVGFNRLMDVEKHYRRKEFLLFPFLER
                     HGITGPPKVMWGKHDEARGLLAGALDALAAAGPMSAAEARGLIQLALRPASEAVAEMV
                     DKESHILLPMCLDTLDDSEWGEIAQQSLEYGFCLYDPPDTWQPAGPAAPAAEGAAAGG
                     RVQLSSGSLAPDELAAILNTIPFDLTFVDKDDTVRYFTQGRERIFARSRAILGRKVQY
                     CHPPSSVKIVEQILEDFRLKRHDRAAFWITMKGRFIHIEYFALRGQDGAYLGCLEVSQ
                     DLTDKRALEGEQRLLSYADRGDGDGS"
     gene            complement(6476..7474)
                     /gene="pgsW"
                     /locus_tag="PLT35_02910"
     CDS             complement(6476..7474)
                     /gene="pgsW"
                     /locus_tag="PLT35_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOG28203.1"
                     /translation="MLAAPCGVRGQAVAPPAGAAPGVPLMATAAQRSAAVLMTRGLAS
                     IKAHRLAKGISIDREIDPNETGIIGDEFTPLTTSLGEAAAKRTSANPAFAAVVARYFE
                     QAGLVPGDVVAIGGSGSFPAFVLAALCAARALDLEPVVIYSIGSSMYGANIPGFTFVD
                     MLAGLRADGVLPYELAAVAPGGERDSGRGVLFDEDGTALVDEARKSRLPIVDGPTLAA
                     RIERRLRIYERAARGRPVRCFVNVGGASANVGDTPASLDLPNGLVLRQPGAPAPPAPG
                     LAFVFLSRGVPVVHLLFVKGLARDNGLPYDPVPLPAVTLPRAVERQAPEPRAGASP"
     gene            7630..9054
                     /locus_tag="PLT35_02915"
     CDS             7630..9054
                     /locus_tag="PLT35_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28204.1"
                     /translation="MRTVRLAGFASLLLALANTAAAQVDPSPLIAPVVFHWVQTQTAW
                     PTLEGGSVGSASQAFWARQDALMETAGFNALLFDVTHGAVASMRNHLAAMRARAEDLP
                     EGPPPLRLVPFIAAEAFAEYSAPKEVLSPAGFEAFYKVIRGFFDMCAEYFPAKPGQKG
                     PLDPAPFARVDGRVFIVLWWVPLQSYDLPRTFFEALSDRLERDFGFRAYWSVHEWFES
                     GGPDDVNYLFNGPKRIQKGRNPKYPAVDLLVAFWPPNVESYASDLFAARNGAAPYAAA
                     WDEVIAANPRPSIVLVESYNEITEGSHLMPSWPVSHWPGDGHWTGPPDDPGCATQPCH
                     PVSYTDTWGPPNPWHYLDVTRRKIREWLEGPVGTADVVPPHAFIIAPRSDDVVSGATP
                     ITILAADDVALREVRVRLDGLLLLTGTGRIDRRLKSWNLSNGPHAISVETIDEAGNRS
                     TVTNEFFVQNPAGTRERAAPPSKR"
     gene            complement(9191..10261)
                     /locus_tag="PLT35_02920"
     CDS             complement(9191..10261)
                     /locus_tag="PLT35_02920"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOG28205.1"
                     /translation="MKPTMPGGRASIAAVAAIAAAALASGAGQTGEKGVRLVRDDANR
                     RVDVIVDGQPFTAYIYPAALKKPVLFPIRTANGTLVTRGFPLEPREGERVDHPHQVGL
                     WFTYGDVNGVDFWNNSDAIKPEDRPKMGTIVHKGVAAVHSGADRGELETDMDWTLADG
                     QVVLKEHTTFTFRGGAGWRSIDRVTRLQALDGRVSLRDNKEGMFGMRVARQLEIPSQK
                     PEVFADAAGRPTSVAVMNNEGVNGDYLTSEGRTGDAAWGTRAKWCALSAKIGSEPVTI
                     AILDHPANPGFPTYWHARGYGLFSANPLGQKELSGGRETLDFALGPRESATFRYRVVI
                     FSVPADAARVEAAHTEFVAAYR"
     gene            complement(10265..>10447)
                     /locus_tag="PLT35_02925"
     CDS             complement(10265..>10447)
                     /locus_tag="PLT35_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28206.1"
                     /translation="ATTANMRNWMECVRSRKTPNADIEAGYSHSVALCMTIAAVQTGQ
                     RIGFDDAKQGVVVGAR"
BASE COUNT         1441 a         3749 c         3601 g         1656 t
ORIGIN      
        1 cgcgggtcag ggcacgcttg cgctcgaaat gctggaagac gcgcctgaca ttgattgctt
       61 tgccgtcccg attggcggcg gcgggctgat ctcgggcatc tcgaccgttg cccgcgcgct
      121 caagcccggc atcgagatcg tgggcgttca ggccgaactt ttcccgagca tgtacaatgt
      181 catcaagggc actgacatgc cctgcgaggg cgatacgctc gccgaaggca ttgcggtcaa
      241 atatcccggc ctcctcacgc gccgcttcgt gcgcgcgctg gtgaatgaca tccagctcgt
      301 tggcgagcgg catctggagc aggcggtcag ccttttgctc cagattgaaa agaccgttgt
      361 ggaaggtgcc ggcgccgccg ggcttgccgc ggcgggtctt gccctccagg agcacgcaga
      421 cgatctgcag cttgagcttc ttcgcctcgg ccatcatggc gttcagccgg gcgatctcgt
      481 cgtcaatcgt gatgccggag gcgccgaggc ccttcgcggt ggacccgagg acgaccatca
      541 gcgtcttgta cggcttggcc gccaggtcgg cgggctgcgg ctcggcgttg tagtcgaagt
      601 ccgacacggc ccccgtgcgc tggaggagca gcctcgccat tttggcgccc ggcccctggc
      661 cggcattggt gatgagcagc ggcgtctgtc ccttcggcgg cgccgcgccg gagaccgccg
      721 ccgccgctga cgccgcaaac gacatcagcg ccgtgactgc gacaagcgag accagccgcg
      781 tgtgcctcat cgccaccctc caggcgccgc gcgggggggc gcggccgaag cggcggcgcc
      841 cggcggcgcc gcctgagccc ggccggcagg cggccgcccg agcgcccgat catcttacta
      901 tcgccccggc gccggagcgg cggtttccgc ggccgatcgc gcggcgggcc cggtcggcgg
      961 ctccccgcca ctcccgctcc gccccgcgct tccagccccg tcgcggtgcc cgtcgggctg
     1021 ccgtcgcggc agcgggcccc gcatccgaac ggcccgtcgg cgccgccccc ggaccgttcc
     1081 cgccgaacct ccgcgccccg gcgctcgtct ctgtctcctg aggccagcgg gcggccgacg
     1141 gggcgccgcg cggaggtggc catgctcgag attcggaacc tgacgaagtc ctacggcgac
     1201 gtcctggccc tcgaccgctt ctcgctcgac ctggcgaagg gcgaggtgct cgggctgctc
     1261 ggcccgaacg gcgcgggcaa gaccacgctc atctccatcc tcgccggcac gctgggcggc
     1321 ttcaccggca cggtgacctt ccaggggcag agcctcttcg ccagccgcca gctcaagaac
     1381 cgcctgggca tcgtgcccca ggacatggcc ttctacgagg acctgagcgc catggacaac
     1441 ctgctcttct ggggcgggct gtacgacgtg ccccgggccg agctgaagcg gcgggccgcg
     1501 gagatcctcg agttggtcga gctggccggc cgcgcgaagg agccggtcaa gaagttctcg
     1561 ggcggcatga agcggcgcct gaacacggcc atcggcctgc tgcacaagcc ggatctcctg
     1621 ctcctcgacg aacccacggt cggcatcgac gtccaggcga aggtcagcat cctggacatc
     1681 atccgcaacg tcggcgcggc gggcaccgcg gtcatcttca cgacgcacca gctggccgag
     1741 gtggaaacgg cctgctcgcg cgtcgccatc atggacaagg gccgcatcct cgcccagggc
     1801 acgctggacg agctggtccg catcgtgggc gagcgcacct tcgtcgagat cagcggggag
     1861 ttcggcgcgg gcgcgttctc tgaggccatc caggacatgc cggcgcccag cgtcgagctc
     1921 atgtcggcgg gcgacaacct ggccctgctc gccgtctcgg acaccgatca aatcccgcgc
     1981 gtcatggacc ggctcttcgg ccgcaagctg gcggtgaccg atctcacgat caagtcgccc
     2041 aacctcgaga ccgtcttcct gaagctgacc gggcgcagcc tgagggactg atcgatgcgc
     2101 cgcctcctcc acgtcttcac gaacgacgtc cgccggcacc tccgcgcgcc gctggtcatc
     2161 gtggtcttca tggccattcc cctggcgatg acggcgctca tcggcgccgt cttcggcccg
     2221 aagcccggcg cgaacgcgct gccgcgcatc gccgtcctcg tcgtggatcg cgacaagagc
     2281 gtggcctcga agctgctcct cggggccttc gactcggccc ggatgaagga gatgttcgag
     2341 gtcacggtca tcggcgaatc cgagggccgg gagcgcatga aggccggcag ggcgtcggcg
     2401 atgatcgtcg tgccggagcg cttcacgctg gaccttatgg aatggaagcc ggtggcgctc
     2461 acggtggtga agaacccggc cgagcagttc ctgccggacg tggccgagga ggcggtgaac
     2521 acgctggcgg tggtcctctc gggcgccgtg cgggcgttcg gcgacgaggt cagggggatc
     2581 cggacgatga tggatctccc gctcgaggcg tttccgtggg cgtcgctcgg gccggagctc
     2641 gcgaaggccc agagcaaggt catcgccgcc tcgaagtacc tcgacccgct gctcatccgg
     2701 ctgaaatcgg aagaggtgac gaccgccggg tcgcgccccg ccgtcacccg ggcggacgtg
     2761 ttctcggcgg tcctgcccgg catggcgatc atgttcctgc tgttcatcgt ccagaccgtg
     2821 atgcgcgaca tcctcgccga gcgcgaggac ggcacgctcc ggcgcatgat gagcgcgccg
     2881 ctgcgcccgc tggaactcgt cggcgcgcgc atcctcgggg gctggctcat gggcctggcc
     2941 gtgctcctcg tcatggcggc cgccgggagg ctgctcttcg gcgcgacgtg ggggccgttc
     3001 ggctacttcc tcctgctcgg cgcgacggcc gccttctggg tcgcggcgtt cttcgcgctc
     3061 ttccacgccc tcgtcgccaa ccgcaaccag gccggcgcga tcggcgcgcc ggtcattctc
     3121 gtcttcagcc tgttcggggg gtcgatgatg agccccgacg cgatgccggc cgcgttcagg
     3181 ccgatcggcc tgttcacccc caaccgctgg ttcatcgacg gcgcggccct cgtccgggac
     3241 ggccggtttc cgctcgccgc cgtcgccgtg ctcggcgcga gcgggctcgc tctgctcgcc
     3301 ctcgccgtgc ccgccctgct gcggcagacc cggagcgcgt catgagaagg ctgggacgcg
     3361 tcctgcacgt ggcccgcaac gacgtgtggc gcatggtccg cgacaggccc ttcttcttct
     3421 ggaccctcgc gttcccgatc ttcttcatcg tcgtcttcgg gatgctgttc aaggcgggcg
     3481 acgccgcgcc ggccgtggcg tcgctgacgg tggtcaacca ggacgaaggg cgctggggcg
     3541 cgtacttcgt cgacaagatc aaggcgccga acatcgagat caccgtcgtc aaggcggagc
     3601 cggccgactt aacccgcctc atcgtcctgc cgcccgactt ctcggcgagg atcgaggcgc
     3661 ggcgcgatca ggccctcgcg ttcaagaagc gcgccggcgc gtcggagaga gccgcggccc
     3721 gcgtcgagac ccgcctgttc caggcgatcg cccgggtcct cagcgagctg gtgctcaacg
     3781 gcgacggcga tctgacgacg ttcctggacg gccacccgga ggtcgcggat ctcgtccggg
     3841 tggagacgcg gttccccgag aagaccgtca cggccgtgcc cagcgggttc gaccactcga
     3901 ttcccggaac gaccgtgcag ttcatcatga tgatggtgat gatctacggc gggatctcgg
     3961 tgatggagga tcggaagaag ggcgtgctga gcaggctcct ctgctcgccg ctctcgaccg
     4021 gcgagctgtt ccaggccaag ctcctggggc ggtggtcgat gggcgtcctg caggcgctga
     4081 tgctcttcgc cgtcggcaag gcgttcttcg ggctcaacct gggaagcgcg ccgctcgcgc
     4141 tgctcgtgct cgccgtgttc gccctcgcgg tggctgcgct tagcgtcttc gccggctcgc
     4201 tgctcacccg cgaggatctc atcgtgggcg tgtccgtgct gctggccaac atgttcgccg
     4261 ggctcggggg ctgctggtgg ccgaacgaga tcgtgccgcc ggccgtgcgg aagctggcga
     4321 tgatctcgcc ggcctactgg gccatggacg cgctgcacgc cctcaggttc ttccagggcg
     4381 gcctgcgcga catcctgcca cacctgggga tcctgctgac gctggcggtc gtcctcgcgg
     4441 tgctcgccgc gcgcacgttc agggtccggc cgtagcggcg cgtccgggtc tcagcgcctc
     4501 gtctcgtccc ttgcgaagta cgtgcgaacc aggtccgacc cctcgcggac gctccaggcc
     4561 cggtatccct tctcggcggc cagggcgacg agcggctcgg ggaggaacgg agtgacgagc
     4621 tcgtagacct ctcccggccg cagcccggcc cagtcccgca tgacctgcgg catgggatgt
     4681 ccgccggcgt caatgacggg acgggcgtcg taggtgcggg ccaccaaggc cggatcggcc
     4741 cagtccggcc gctccaacgc tgtctcatcc gcctcgtcaa cgcaggaggc cggggcctgc
     4801 cccgcatcgg cgcgcagccg gttgatgagg gtgggcagtg gcacgtttcc gactttggcc
     4861 acctggcgga gcgtcgccac cttggccacc gtgcgcctga ggacggggtt cttcagcgcg
     4921 ccgtaggccg gcgacagggc gaggaggctc gcctcgagct ccgggtagtg ggcgagcagg
     4981 ctcccgaccg tggtttcggg cgcgatcggc ggccgcgcgg tctcagctcc catcgccgtc
     5041 cccgcgatcg gcgtaactca gcaggcgctg ttcgccttcg agggcgcgct tgtccgtcag
     5101 gtcctggctg acctccaggc accccaggta cgcgccgtcc tgtccgcgga gcgcgaagta
     5161 ctcgatatgg atgaagcgcc ctttcatggt gatccagaac gccgcgcgat cgtggcgctt
     5221 cagtcggaag tcctcgagga tctgctccac gatcttgacg ctcgacggag gatggcagta
     5281 ctgcaccttc cggcccagga tcgcccggct gcgggcgaag atgcgctcgc ggccctgcgt
     5341 gaagtaccgc acggtgtcgt ccttgtcgac gaaggtgaga tcgaacggaa tcgtgttgag
     5401 aatggcggcc agttcgtccg gggcgaggct cccggacgac agctgcaccc ggccgccggc
     5461 agccgcgccc tcggcagccg gcgcggcggg gccggcaggc tgccaggtgt caggcgggtc
     5521 gtagaggcag aacccgtact cgaggctctg ctgagcgatc tcgccccact ccgagtcgtc
     5581 gagcgtgtcc aggcacatcg gcaggaggat gtgcgattcc ttgtcgacca tttccgcgac
     5641 ggcctccgac gccggcctga gggccagctg gatgaggccg cgggcctcgg cggcgctcat
     5701 cgggccggct gccgccaggg cgtccagcgc gccggcaagg aggcccctgg cctcgtcgtg
     5761 cttgccccac atgaccttcg gcgggccggt gatgccgtgg cgctcgagga agggaaagag
     5821 caggaactcc ttgcgccggt agtgcttctc cacgtccatc agccggttga accccacgcg
     5881 gatcgccgcc acgatctcgg acgcgtcggc gtcggcgggt gccgagtcgg ctgcggcgta
     5941 gaggcgggag agctcgtcga cctcccacag cagcgccttg ttctcctcgg cgaacgtgtg
     6001 cgccggatgg cccggaggcg cttccggggc ggacctcagg tcgatggcgc ccttgagcgc
     6061 ctctccgtgc agatcgcaca ggcgcaggat ctcctccgtg ggcacgcctt cgttcacgag
     6121 ttcctgctcc acctcgacca cgtcgctgta aggaacctgg cccatgaggc ggacgatccg
     6181 ggtgcggacc tggtccggcg ccgcgccctg atggatctgc aggatcatgt gcttcagcag
     6241 gtccttgcgc cgcctcgcgt gctcgatcaa ctcgctcatc agcgtctcct tcagcgccgg
     6301 ctcccccgct tccgggaacc gggcggttcg cgcaatccag gccccgatgg gcacgtcccg
     6361 cgcggcggga ggcgaagccg ctcccgcccc cccgcgcggc ggggcgcccg tgccggtatg
     6421 accgcaggct gaagtcccat cctactatgc gcccggcggc cgctgcgggc cgttctcacg
     6481 gcgaggcgcc tgcccggggc tcgggcgcct ggcgttcgac ggcccgcggg agggtgaccg
     6541 ccggcaacgg cacggggtcg tacggcagcc cgttgtcgcg cgcgaggccc ttcacgaaca
     6601 gcaggtgaac gaccggcacg ccgcgcgaca ggaacacgaa cgcgaggccg ggcgcgggcg
     6661 gggccggggc ccccggttgc cggagcacga ggccgttcgg cagatcgagc gatgcgggcg
     6721 tgtcgccgac gttcgccgac gcgccgccca cgttgacgaa gcaccgcacc ggccttcccc
     6781 gcgcggcgcg ctcgtagatg cgcagccgcc gctcgatccg tgcggccagc gtcggcccgt
     6841 cgacgatcgg cagccggctc ttgcgggcct cgtcgacgag ggccgtcccg tcctcgtcga
     6901 acagcacgcc gcgaccggag tcgcgctcgc ctcctggcgc tacggccgcc agctcgtagg
     6961 gcagcacgcc gtcggcgcgc agccccgcca gcatgtccac gaacgtgaac ccgggaatgt
     7021 tggcgccgta catcgaggac ccaatcgagt agatgacgac gggctccagg tcgagcgccc
     7081 gcgcggcgca gagcgcggcg agcacgaacg ccggaaacga tccgctgcca ccgatggcca
     7141 cgacgtcgcc gggcacgagg ccggcctgct cgaagtagcg cgccacgacg gccgcaaacg
     7201 ccgggttggc cgacgtccgt ttcgccgcgg cctcgccgag ggaggtcgtc aggggcgtga
     7261 attcgtcgcc gatgatgccc gtctcgttgg gatcaatctc gcggtcgatt gagatgccct
     7321 tcgcgaggcg gtgcgccttg atgctcgcga gccctcgcgt catgaggacg gcagccgagc
     7381 gttgcgcggc cgtggccatg agcggcacgc cgggagcggc gccggcgggt ggcgccacgg
     7441 cctgcccgcg cacgccgcac ggcgcggcca gcatcccggc aacaaggggc gccaggggta
     7501 caatggcacg tgacaggacg cgagcgctgg gcatggcgga caggcgccgg gccagggggc
     7561 ccggcgcgca tggcctcatc ctacccgact cgagagcccg gcttgaatgc cgccaacctg
     7621 agtgaagcca tgcgcacggt ccgcctcgcc gggttcgcct ccctcctgct ggcgctggcg
     7681 aacacggccg cggcgcaggt cgatccgagc ccgctcatcg cgcccgtcgt cttccactgg
     7741 gtccagacgc agaccgcgtg gcccacgctc gagggaggct ccgtcggaag cgcctcgcag
     7801 gcgttctggg ctcgccagga cgccctgatg gagacggccg gcttcaacgc gctgctcttc
     7861 gacgtgaccc acggggccgt ggcctcgatg cgcaatcacc tcgctgccat gcgcgcgcgg
     7921 gctgaggatc tgccggaggg cccgccgccg ctccggctcg tccccttcat cgcggccgaa
     7981 gcgttcgccg agtactcggc gccgaaagag gtgctgtcgc ccgccgggtt cgaggcgttc
     8041 tacaaggtca tcaggggctt cttcgacatg tgcgcggagt acttcccggc gaagcccggc
     8101 cagaaggggc cgctcgatcc ggccccgttc gccagggtgg acggccgcgt cttcatcgtc
     8161 ctgtggtggg tgccgctcca gagctacgac ttgccgcgca cgttcttcga ggcgctcagc
     8221 gaccggctgg agcgcgactt cgggtttcgc gcgtactgga gcgtgcacga gtggttcgag
     8281 tcgggcggac ccgatgacgt gaactacctg ttcaacgggc ccaagcgaat ccagaaaggc
     8341 cgcaacccga agtacccggc cgtggatctc ctggtggcgt tctggcctcc caacgtcgag
     8401 agctacgcca gcgacctctt cgccgcacgg aacggggccg cgccctacgc agcggcctgg
     8461 gacgaggtca tcgccgccaa cccgcggccg tcgatcgtgc tcgtcgagtc ctacaacgag
     8521 atcaccgaag gcagccacct gatgccgtca tggcccgtca gccactggcc gggcgacggc
     8581 cactggaccg ggccgcccga cgatcccggc tgcgcgacgc agccctgcca cccggtcagc
     8641 tacacggaca cgtgggggcc gccgaacccc tggcactacc tcgacgtcac ccggcgcaag
     8701 atccgcgagt ggctggaggg gcccgtcggc acggccgacg tggtgccgcc gcacgcgttc
     8761 atcatcgctc cccgctcgga cgacgtcgtg tcgggggcca cgccgatcac gattctggcc
     8821 gccgacgacg tcgccctgcg cgaggtgcgc gtccgcctgg acggcctcct gctgctcacg
     8881 ggcacgggga gaatcgacag gcggctgaag agctggaacc tgtccaacgg ccctcacgcc
     8941 atctcggtgg aaacgatcga cgaggccggc aaccgcagca ccgtcaccaa cgagttcttc
     9001 gtccagaacc ccgccgggac gcgggagcgg gcggcgccgc cttcgaaacg gtgaccgccg
     9061 ccaattcgcc gggttgtccg ccgatcacgt gacgctcgat catgtgacgc tttccaattc
     9121 gatcagccag cggaattgga aagcgcctcc tcttcaccgg aattggagaa tgtctcctct
     9181 ctcgtgagcg ctaccggtaa gccgcgacga actccgtgtg cgccgcctct acgcgggcgg
     9241 cgtcggcggg caccgagaag atcaccaccc ggtaccggaa cgtcgcgctc tcccgcgggc
     9301 cgagtgcgaa gtccagcgtc tccctgccgc cgctgagttc cttctggccg agggggttgg
     9361 ccgagaacag cccgtagccg cgcgcgtgcc agtatgtcgg gaagccgggg ttcgccgggt
     9421 gatcgaggat cgcgatggtc accggctcgc tgccgatctt cgcgctgagc gcgcaccact
     9481 tcgcgcgcgt tccccacgcg gcgtctcccg tcctgccctc gctcgtcagg tagtcgccgt
     9541 tcacgccctc gttgttcatc accgcgacgc tcgtgggcct gccggccgcg tcggcgaaca
     9601 cctccggctt ctgcgagggg atctcgagct ggcgcgcgac gcgcatgccg aacatgcctt
     9661 ccttgttgtc cctcagcgac acgcgcccgt cgagggcctg caggcgcgtg acgcggtcga
     9721 tcgacctcca gcccgcgccg ccgcggaacg tgaacgtcgt gtgctccttc agcaccacct
     9781 ggccgtcggc gagcgtccag tccatgtccg tctccagctc gcccctgtcg gcgccgctgt
     9841 gcacggcggc cacgcccttg tggacgatgg tgcccatctt cgggcggtcc tccggcttga
     9901 tggcgtcgga gttgttccag aaatcgacgc cgttcacgtc gccgtaggtg aaccagagcc
     9961 ccacctggtg cgggtggtcc acccgctcgc cctctcgcgg ctccagcggg aagccccgcg
    10021 tcacgagcgt gccattcgcc gtccggatcg ggaacagcac gggcttcttc agggccgccg
    10081 ggtagatgta cgccgtgaac ggctgcccgt ccacgatgac gtccacccgc cggttcgcat
    10141 cgtcgcgaac gaggcggacg cccttctcgc ccgtctggcc cgccccgctc gcgagcgccg
    10201 ctgcggcgat cgcggcaacc gccgcgattg acgcacgacc gcccggcatc gtcggcttca
    10261 tggctcacct cgctcccacg acgacgccct gcttcgcatc gtcgaacccg atgcgctgcc
    10321 ccgtctgcac ggccgcgatg gtcatgcaca gggccaccga gtggctgtag ccggcctcga
    10381 tgtccgcgtt cggcgtcttc cggctcctga cgcactccat ccagttgcgc atgttcgcgg
    10441 tcgtcgc
//
LOCUS       DAOSIF010000047        10387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6279_length_10387_cov_4.279907, whole genome shotgun sequence.
ACCESSION   DAOSIF010000047 DAOSIF010000000
VERSION     DAOSIF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6279_length_10387_cov_4.279907"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..580)
                     /locus_tag="PLT35_02930"
     CDS             complement(8..580)
                     /locus_tag="PLT35_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG28207.1"
                     /translation="MSDARSPAPTAAAPARRNVAIIAHVDHGKTTLVDALLAQSGAFR
                     ANERVAERALDNIALERERGITIMAKNTAVHYGGVLVNIVDTPGHADFGGEVERTLAM
                     VDGVLLLVDASEGPLPQTRYVLRKALERRLPPIVVINKIDRQDARPSEVLNEVYDLFI
                     DLDASEDQLGFPVLYTNARAGTAVFHFEHA"
     gene            652..1461
                     /locus_tag="PLT35_02935"
     CDS             652..1461
                     /locus_tag="PLT35_02935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG28208.1"
                     /translation="MPDSAPVRTALVTGASAGIGQAIAEVFAKEGFDLVLTARRGDRL
                     EALAADLRQRFGRRVATIVADLADPAAPARIVGEIAAAGITVDALVNNAGYGVPGKFT
                     DTPWEAQRDMLQVMVMAVAELTHRLLPGMIQRRYGRIINIGSVAGLVPQSAGHALYGS
                     SKALVTGFSQSIALEWMPHNVYATAVCPGFTYTEFHDVNHMREQVSRLPKFMWMDADT
                     VAREAFEAVMAGRILHVPGRFYRMVVFLARYTPRWLVLAVVKRVSKTYRRT"
     gene            1474..2676
                     /locus_tag="PLT35_02940"
     CDS             1474..2676
                     /locus_tag="PLT35_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG28209.1"
                     /translation="MATLGPAALVASLIALGLAERRARDLARRAVPLRIHVNGTRGKS
                     TVTRLLAGALRAGGIRCLAKTTGTEPRLILPDGSERAIRRRAPASIREQLWAMRQARA
                     LGARAVVLECMAVEPELQAVAERDMIRATIGVITNARLDHAEAMGATVEDVAAALALT
                     IPERGTIVVGPTGGADVIAAAARAKGARLVVAAAESAPPGPDVNPGPDAGLGTHPDLG
                     PEAGSRPDWCADNAGIALAVTRLLGIPDDVARSGMREAAPDPGAAQRGAVDAAGRRVE
                     YLDASAANDPESLDRLLTPRTGQALFVFNHRMDRPLRLRQFAEAEPWSRDTDRLVVTG
                     DRPDWLTWRRLRRRLPGGRLAFERPSRLAARLAADLRADAGATLLVFCGNTKGFGREP
                     LLAALRRR"
     gene            2678..3118
                     /gene="pgsC"
                     /locus_tag="PLT35_02945"
     CDS             2678..3118
                     /gene="pgsC"
                     /locus_tag="PLT35_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOG28210.1"
                     /translation="MVELALGLGIALNLLFTEVFGLASGGLVVPGYLALHLSQPARVL
                     STIAVGWLTYAAVRYGLARLIILYGRRRFGVTVLTGFVLHGVCTWAIASAPAPADLRV
                     IGYIVPGLLANTALSQGFWATAGTTLVLAVLVRFLLAALSGVLL"
     gene            3130..4092
                     /gene="pgsW"
                     /locus_tag="PLT35_02950"
     CDS             3130..4092
                     /gene="pgsW"
                     /locus_tag="PLT35_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOG28211.1"
                     /translation="MPARPGIVRLPPWVPAAAGVAIAAILAAQAVGWLALPRWLAPWE
                     PPAAPGVEQRAERAARIWREAAGEIARARGVSADEALIGSELTPLVTTLGALEAKRVA
                     ASPAWAPALAEQLHAAGIGPGDTVAASFSGSFPGLNLAVAAACEALDARLVAVSSVTA
                     SSFGATDPGFTWPEIEARLVGSGALRPVSAAVSAGGDGDRALDLDEEGRALAARIAAD
                     AARELGARLLEPSTFEEAVAARIAVFDAARGARRLAAFVNVGGTEASLGLSPAILRVR
                     SGWLQPVVFDRSPGRGLVARMVERGVPVLHVLNVRELAVRWGVR"
     gene            complement(4120..4344)
                     /locus_tag="PLT35_02955"
     CDS             complement(4120..4344)
                     /locus_tag="PLT35_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28212.1"
                     /translation="MTASIWRRLGCCLSGHDYSVKSDGTRMFLRCRVCGRTSRGLIMG
                     EDPCRRRSRHEQSAAQDQRPAERRTPLSAR"
     gene            complement(4786..6960)
                     /locus_tag="PLT35_02960"
     CDS             complement(4786..6960)
                     /locus_tag="PLT35_02960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG28213.1"
                     /translation="MLHGLPDDDARAILWRFGGCEDLRRLAVAARGVARGTVARLVAA
                     GGRLTHEWTPEKQAMLDAFDEAGLGGPSGEDAAGAQAAHRRSLASALTAFELAWVDAG
                     AATTYLACVLAAEPIRMFGTERQRANYAGAAPGGRARRGAFALTEPIPDAGVETGLLG
                     GTVDVAEWGGGREPVLRVRKRGRFITGMAFAEFVTAAVGSADERIKGTCAVILEAGDP
                     GVFDRGLPARTLVHQLSSSRNPVFDVRVPADRILGGYDVENGVIVPRVTHGDIVEAVF
                     RRTRVTVGLMTAAKLLSAVEPIIRYQRRRFRGSRTVQPGSPRYELGLQQKEDVLHRLV
                     DVWATGEAAASLGFAAARALDELDQLAAEGQPAGAGTADLVEEYFAEAARPAHKRSPE
                     RLAELGRDPRVRATLLEAVAGVLSPAVKLWNTGHGANVIREAVSLLGGQGLTEDSPGF
                     LGQKWMDAQLEATYEGPEAVQRRQLAHAMIGEVFLRQFESWVEEMRALASQRPGTGTC
                     ALATAMGLWLWTLRHLQRATDPAGAKLYQASRQGVSFPMADALAWLLAARQQALDVVE
                     LERRTRDDAASAGRWRDLVPILMDLCHVQAARAASEAARICTDLVYGYRRHPSWDDAG
                     ASACFGGAEIESLEEVIPGMAATARFYADVIEVDGSHPAKAGPCTSVEGLQPFERLRA
                     QLGRCLTGSRLAKDRAADALTRVEIADAPDYPRPADDPAGAR"
     gene            7189..8202
                     /locus_tag="PLT35_02965"
     CDS             7189..8202
                     /locus_tag="PLT35_02965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG28214.1"
                     /translation="MTDDLSRRTFVKLGLTGAAALAVRPALGAAAPPMPERPLGRTGH
                     MVRLFSLGGQASLEQPDKHDVSMAIINRAIDLGVNYIDTAAAYGRPRAADTPRWKLDG
                     ISQTFIGEVMATRRKEVFLASKTDDRTRDGSLRLLEQSLKLLRTDHLDLWQLHNVMRD
                     EQVEQIFASGGAIEALLKARDDKMVRFLGITGHYDPAPLIKALERFEFDAILMALNPA
                     DPHRFPFKTELLPLANRKKMGVIGMKIPARDRLLRPDGVSTMKEAMSYTLTLPVSTVV
                     VGCKTVEQLEENIAIARSFTPLPPAEMARLEGLTASYVADAAWFRRPPAAGGPVENDQ
                     NTD"
     gene            8311..9324
                     /locus_tag="PLT35_02970"
     CDS             8311..9324
                     /locus_tag="PLT35_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOG28215.1"
                     /translation="MQAIVLRELGGPERLVLETAADPAPGPGEAVVRLRAAALNRRDA
                     WIRQGQYAGIRLPIILGSDGAGEVTAVGEGVDRAWPGTAVVINPSLEWGPDSRAQGPS
                     FRILGLPDHGTYAELVKVPAANLYRKPASLSWEAAAAIPLAGLTAYRALVTRARLQPG
                     ETVLVTGVGGGVATFVLLFALHLGARVVVTSGSDAKIARARELGAAGGANYRLDGWGQ
                     AIARLTDGGPDVIVDSAGREAFPTLLEVVRPGGRIVTFGATTGSASTVEVRRVFWKQV
                     DILGSTMGTPEEFSSMLALFDRGLEPVIDRTFPLAAAADAHRRMDQADQFGKLVLTIQ
                     PQA"
     gene            complement(9669..10262)
                     /locus_tag="PLT35_02975"
     CDS             complement(9669..10262)
                     /locus_tag="PLT35_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28216.1"
                     /translation="MPLPFRAGAPGRAEAPRGEGAGNAPARLGTGVVAALIVALGVAA
                     AGAYCWKRGALLEMLPAARPAALSGEGRTGTAPGRQTGTASAPSPDPTFRGVKYLART
                     DDGPREFGAILVFASDRLRVTPEGGGAALRSVRYRDIAAASCSRIERKRLGLLSSTRL
                     VLAVETAGEPLLLRIDEDHAEAILEALEARWGKAVGR"
BASE COUNT         1361 a         3823 c         3754 g         1449 t
ORIGIN      
        1 acaagggcta tgcgtgttcg aagtggaaga cggccgtgcc cgcgcgcgcg ttggtgtaga
       61 gcacggggaa gccgagctga tcctcgctcg cgtcgagatc gataaagagg tcgtacacct
      121 cgttcagcac ctcgctcggc cgcgcgtcct gccggtcgat cttgttgatg accacgatcg
      181 gcgggagccg ccgctcgagc gccttgcgga ggacgtaccg cgtctgcggc agcggacctt
      241 cggacgcatc gacgagcagc agcacgccgt ccaccatggc gagcgtgcgc tccacctcgc
      301 ccccgaagtc cgcgtggcct ggcgtgtcga cgatgttgac gagcacgccg ccgtagtgga
      361 cggcggtgtt cttggccatg atcgtgatgc cgcgctcccg ctcgagggcg atgttgtcga
      421 gggcgcgctc ggccacccgc tcgttggcgc ggaacgcgcc gctttgcgcg agcagcgcgt
      481 ccaccagcgt cgtcttgccg tgatcgacgt gggcgatgat ggcgacgttt cgccgggcgg
      541 gggcggccgc cgtcggcgcg ggggaccggg cgtccgacat cccctcagta tagcggccgg
      601 aggaggcccg ccgcctccgg acgtcccggc ccttcgtgta gactgtcggc catgcccgac
      661 tccgcgcccg tccggaccgc gctcgtcacc ggcgcctcgg ccggcatcgg ccaggccatc
      721 gccgaagtgt tcgcgaagga aggcttcgac ctggtcctca cggcccggcg gggagaccgc
      781 ctcgaggccc tcgcggcgga cctccggcag cgcttcgggc gccgcgttgc gacgatcgtc
      841 gccgacctgg cggacccggc ggcgccggcc cggatcgtcg gggagatcgc ggccgccggc
      901 atcacggtgg acgccctcgt caacaacgcc ggctacggcg tgccggggaa gttcacggac
      961 acgccgtggg aggcgcagcg cgacatgctc caggtcatgg tgatggctgt cgccgagctg
     1021 actcaccgcc tgctgcccgg catgatccag cggcgctacg gccgcatcat caacatcggc
     1081 tccgtcgcgg gcctggtgcc gcagtcggca gggcacgcgc tctacgggtc cagcaaggcg
     1141 ctcgtcaccg gcttctccca gtcgatcgcc ctcgagtgga tgccccacaa cgtctacgcc
     1201 acggccgtgt gccccggctt cacctacacc gagttccacg acgtcaacca catgcgggag
     1261 caggtgagca ggctgccgaa gttcatgtgg atggacgccg acaccgtcgc ccgcgaggca
     1321 ttcgaggccg tcatggccgg gcggatcctc cacgtgcccg gccgcttcta ccgtatggtg
     1381 gtgtttctgg cgcgctacac gccgcgctgg ctggtcctgg ccgtcgtgaa gcgcgtctcg
     1441 aagacctacc gcaggacctg acgggccttc gccgtggcca cactcggccc cgccgcgctc
     1501 gtcgcctccc tgatcgcgct gggcctcgcc gagcgcaggg cgcgcgacct ggcgcgtcgc
     1561 gcggtgccgc ttcggatcca cgtcaacggc acgcgcggca agagcaccgt gacgcgcctc
     1621 ctcgccggag cgctgcgggc gggcggaatt cgctgcctgg ccaagaccac cggcacggag
     1681 ccgcgcctca tcctcccgga cgggagcgag cgcgccatcc gccggcgcgc gcccgccagc
     1741 atccgcgagc agctgtgggc gatgcggcag gcccgggccc tgggagcgcg cgccgtcgtc
     1801 ctcgagtgca tggccgtcga gccggaactc caggccgtcg ccgagcgcga catgatccgc
     1861 gccacgatcg gcgtgatcac caacgcgcgt ctcgaccacg cggaggccat gggcgccacc
     1921 gtcgaggacg tggcggccgc gctggccttg accatcccgg agcgcgggac gattgtcgtc
     1981 ggccccacgg gaggggctga cgtgatcgcg gccgccgcgc gcgcgaaggg agcccgcctc
     2041 gtcgtcgcgg ccgcagagtc ggccccgcct ggccccgacg tcaatcccgg ccccgacgcg
     2101 ggtctcggca cgcatcccga tctcggcccc gaagccggct cccgccccga ctggtgcgcc
     2161 gacaatgccg gcatcgcgct ggccgtcacg cgcctcctcg gcattccgga cgatgtcgcc
     2221 aggagcggca tgcgcgaggc ggcgccggac ccgggcgcgg cgcagcgcgg cgcggtcgac
     2281 gccgccggcc ggcgcgtcga gtacctcgac gcctcggccg ccaacgatcc ggagtcgctc
     2341 gatcggctgc tgacgccgcg gaccggacag gcgctcttcg tgttcaatca ccgcatggac
     2401 cgcccgctgc gcctgcgcca gttcgccgag gccgagccgt ggtcccgcga caccgatcgg
     2461 ctcgtcgtca cgggcgaccg gccggactgg ctcacctggc ggcggctgcg ccggcggctc
     2521 cccggcgggc ggctggcgtt tgaacgcccg tcgcggctgg cggcgcggct ggccgcggac
     2581 ctgcgcgccg acgccggcgc cacgctcctg gtcttctgcg gcaacacgaa gggcttcggc
     2641 cgcgagcccc tgcttgcggc cctgcggcgg cgctgacatg gtcgaactgg ctctgggcct
     2701 cgggattgcg ctgaacctgc tcttcacgga agtcttcggc ctggcctcgg gcggcctggt
     2761 cgtgccaggc taccttgccc tccacctgtc gcagcctgcg cgggtcctct ccacgatcgc
     2821 ggtgggctgg ctgacctacg ccgcggtgcg ctacggcctg gcgcgcctga tcatcctgta
     2881 cggccgccgc aggtttggcg tgacggtcct gaccgggttc gtgctgcacg gggtctgcac
     2941 gtgggcgatc gcctcggccc cggcgccagc cgaccttcgc gtcatcggct acatcgttcc
     3001 cggcctcctc gcgaacacgg cgctcagcca gggcttctgg gcgacggccg gcacgacgct
     3061 cgtcctggcc gtgctcgtgc ggttcctgct cgcggccttg agcggcgtgc tgctgtaggg
     3121 acgcaaggca tgccggcccg cccgggcatc gtccgcctgc cgccgtgggt gccggccgct
     3181 gccggtgtgg ccatcgcggc catcctcgcc gcgcaggccg tcggctggct cgcccttccc
     3241 cgctggctgg cgccctggga gcctccggcg gccccgggcg tggagcagcg cgccgagcgt
     3301 gccgcacgca tctggcgcga ggcggcgggc gagatcgccc gcgcgcgggg cgtctcggcc
     3361 gatgaggcgc tcatcggcag cgaactcacg cctctcgtca cgacgctcgg cgcgctcgaa
     3421 gccaagcggg tggcggcgtc cccggcatgg gccccggcgc ttgccgagca actccacgcc
     3481 gcgggcatcg ggcccggcga caccgtggct gcttccttct ccggctcgtt tcccggcctc
     3541 aatctcgccg tggcggccgc gtgcgaggcc ctggatgcca ggctggttgc cgtctcgtcc
     3601 gtcactgctt cgtccttcgg cgcgaccgac ccggggttca cgtggcccga gatcgaggcg
     3661 cgcctggtcg gaagcggggc gctgcggccc gtgtcggccg ccgtgtcggc gggcggcgac
     3721 ggggatcgcg cgctcgacct ggacgaggag ggacgcgcgc tggccgcccg catcgccgcc
     3781 gacgcggccc gcgagctggg tgcgcgcctg ctcgagccgt ccacgttcga ggaagccgtg
     3841 gccgcgcgga tcgcggtctt cgacgccgcg cggggcgcga ggcgtctcgc ggccttcgtc
     3901 aacgtcggcg gcaccgaagc gagcctcggg ctgtcgccgg cgatcctgcg cgtgcggagc
     3961 ggatggcttc agccggtcgt cttcgaccgg tcgcccgggc gcgggctcgt ggcccgcatg
     4021 gtggagcgcg gcgtgccggt gctccacgtg ctcaacgtgc gcgagttggc cgtgcggtgg
     4081 ggcgtgcgct gaacgccggg ccggatggcc ggctgaacgt cagcgcgccg acagcggagt
     4141 gcggcgttcg gccgggcgct ggtcctgcgc ggccgactgc tcgtgccgcg accggcggcg
     4201 gcaggggtcc tcgcccatga tgagcccgcg cgacgtgcgc ccgcacactc ggcagcgcag
     4261 gaacatccgc gtcccgtccg acttgaccga gtaatcatgg ccgctgaggc agcacccaag
     4321 ccgccgccag atcgacgcgg tcattgcctt cccccagggc tggagtgctc gagcggcagg
     4381 ccgcccggcg ccactttcta acgcaagtcc agtgttgtca agggaatccg ggcagcccag
     4441 gccagattcc gcgcaaatcc gtgcatccgt gtgcagccga cggggctgac cggaaggtcg
     4501 cgacagccgt acgcgctccc gagcccccgg ctgcgggcag gtcgctcgat cacgagggag
     4561 ccggccgcgt cgatgacggc gattgcgcat gacaacatcc cctggcttcc gcttgcagcg
     4621 gatcgtggcg accggccgct cgggcaggtg atctcgcgac ggacccaggg cgctcagcga
     4681 tcggccgaca gtgcggaacc gtcgggaatg ccggatgggg ggatgggtcg aaccggggca
     4741 tcggcggcgc gatgggatgg ggccgctccc cggggcggcg ccgtgctacc gagcgcctgc
     4801 cggatcgtcc gccgggcggg gatagtcggg agcgtcggca atctccacgc gcgtgagcgc
     4861 gtcggcagcc cgatccttgg cgaggcgcga cccggtcagg caccggccga gctgcgcgcg
     4921 cagccgctcg aacggctgaa gcccctcgac gctcgtgcac gggcccgcct tggcagggtg
     4981 cgagccgtca acctcgatga cgtcggcgta gaacctggcg gtggccgcca tgcccggaat
     5041 gacttcttcg agcgactcga tctcggcgcc gccgaagcac gccgacgcgc cggcatcgtc
     5101 ccagctcgga tgccggcggt acccgtagac gaggtcggtg cagatgcggg ccgcctcact
     5161 ggccgcgcgc gcggcctgga cgtggcacag atccatcagg atcggcacga gatcgcgcca
     5221 gcggccggcc gacgcggcat catcccgagt gcggcgctcc agttcgacga cgtcgagggc
     5281 ctgctggcgc gcggccagga gccaggccag cgcgtccgcc atcgggaagc tcactccttg
     5341 tcgcgacgcc tgatagagct tcgcgccggc tgggtccgtg gcgcgctgca ggtggcggag
     5401 cgtccagagc cagagcccca tggcggtggc cagcgcgcag gtccccgtcc cgggtcgctg
     5461 cgacgcgagc gcgcgcatct cttccaccca ggactcgaac tggcgcagga acacctcgcc
     5521 gatcatcgca tgcgccagct ggcggcgctg cacggcctcc ggcccctcgt aggtggcctc
     5581 gagctgcgcg tccatccact tctggccgag gaagcctgga ctgtcttccg tcaggccctg
     5641 cccacccagc aggctcaccg cctcccggat gacgttcgca ccgtgccccg tgttccagag
     5701 cttcacggcc ggcgacagga ctcccgccac cgcctccagc agggttgccc gcacgcgggg
     5761 gtcgcgcccg agttccgcca accgttccgg cgagcgcttg tgcgcgggcc tggcggcctc
     5821 tgcgaagtac tcctcaacca ggtcggccgt gccggcgcct gccggttgtc cttccgccgc
     5881 gagctggtcg agttcgtcaa gggcccgggc cgcggcgaag cccagcgagg ccgccgcctc
     5941 tcccgtcgcc cagacgtcga ccagacggtg caggacgtcc tctttctgct gcaggcccag
     6001 ctcgtagcgc ggcgaccccg gctgcaccgt gcggctcccg cggaagcggc ggcgctggta
     6061 gcggatgatt ggctcgacgg ccgacaggag cttggcggcg gtcatcaggc cgaccgtcac
     6121 gcgggtgcgg cggaagacgg cctcgacaat gtcgccgtgc gtcaccctcg gcacgatgac
     6181 gccgttctcg acgtcgtagc cgcccaggat ccgatcggcg ggcacccgca cgtcgaagac
     6241 gggattgcgg ctcgacgaga gctggtggac gagcgtccgc gccggcagcc cgcgatcgaa
     6301 gacgccgggg tccccggcct cgaggatcac ggcgcaggtg cccttgatgc gctcgtcggc
     6361 ggagccgacc gccgcggtga cgaactcggc gaacgccatg cccgtgatga accggccgcg
     6421 cttccggacg cggagcacgg gttcgcgtcc cccgccccac tcggcgacgt cgaccgtgcc
     6481 gccgaggagg ccggtttcga cgccggcgtc cgggatgggt tccgtcagcg cgaaggcgcc
     6541 gcgcctggcc cgcccccccg gcgcggcacc ggcgtagttc gcgcgctggc gctcggtgcc
     6601 gaacatgcgg atgggctcgg cagcgaggac gcacgcgagg tacgtggtgg ccgctccggc
     6661 atccacccag gccagctcga aggcggtcag cgccgacgcc aggctccgcc ggtgcgcggc
     6721 ctgcgcgccg gccgcgtctt cgcccgaagg tccgccaagg cccgcctcgt cgaaggcgtc
     6781 gagcatcgcc tgcttctccg gcgtccactc gtgggtgagc cggcctccgg cagccacgag
     6841 gcgcgccacc gtcccccgcg cgacgccgcg ggccgccacc gccaggcggc gaagatcctc
     6901 gcaaccgccg aaccgccaga ggatggcgcg cgcgtcgtca tcgggcaggc cgtgcagcat
     6961 ctgagtcgtc cttccgtgag gggccgcgcc gcgggcagga cgcggagacg aggactaacg
     7021 tacttccgtt ccacgggggg atcaatggtc cgccccgcct gccccgtccg acgcacccgt
     7081 ccgcccgtcc cgcgagcgcc ggtcgtccgc gcccgctcca ggcccgtccg tttcgcgttg
     7141 ccgccgtccc ggcctcgggt tatggtggtg caaaccaagg agcgcgccat gaccgacgat
     7201 ctctcccgcc gcacgttcgt gaaactcggc ctgactggcg cggcggccct cgccgtccgt
     7261 cccgcgctcg gcgcggcggc cccgcccatg cccgagcggc cgctcgggcg gaccggccac
     7321 atggtgcgcc tgttcagcct ggggggccag gcctccctcg aacagccgga caagcacgat
     7381 gtgtcgatgg cgatcatcaa ccgcgcgatc gacctcggcg tgaactacat cgacacggcg
     7441 gccgcgtacg gccggccccg cgcggccgac acgccgcgct ggaaactgga cggcatcagc
     7501 cagacgttta tcggcgaggt catggctacg cggcgcaagg aggtcttcct cgcgagcaag
     7561 accgacgacc ggacgcgcga cggctccctc cggctgctcg agcagagcct gaagctgctg
     7621 cggaccgatc acctggacct ctggcagctg cacaacgtca tgcgcgacga gcaggtcgag
     7681 cagatcttcg caagcggcgg ggccatcgag gcgctcctca aggcgcgcga cgacaagatg
     7741 gtgcgcttcc tggggatcac cggccactac gaccccgctc cgctcatcaa ggcgctcgag
     7801 cggttcgagt tcgacgccat cctgatggcg ctcaatcccg ccgacccgca ccgcttcccg
     7861 ttcaagacgg agctgctgcc gctcgcgaac cggaagaaga tgggcgtcat cggcatgaag
     7921 atcccggcgc gggaccgcct cctccgtccc gacggcgtgt cgacgatgaa ggaggcgatg
     7981 agctacaccc tgacgctgcc cgtcagcacg gtcgtcgtcg ggtgcaagac cgtcgagcaa
     8041 ctggaggaga acatcgcgat cgcgagaagc ttcacgccgc tgcccccggc cgagatggcc
     8101 cgcctcgagg gcctgacggc gagctacgtg gccgacgccg cgtggttcag gcggccgccg
     8161 gcggcgggcg gcccggtgga aaacgatcag aacacggact gatcgggact cggggtccgg
     8221 gtttcgtgac tcgtggtccg ggtttcgtga ctcggggccc gagtttcgcg actcgaggcc
     8281 cgggcttcgg gattcggggg aggaaacgcg atgcaagcca tcgtccttcg tgaactcggc
     8341 ggaccggagc ggctcgtgct ggaaacggcg gcagacccgg ctcccggccc gggagaggcc
     8401 gtcgtccgcc tgagggcggc ggccctcaac cgccgcgacg cgtggatccg gcagggacag
     8461 tatgccggca tcaggctccc catcatcctc gggtccgacg gagcgggaga ggtcaccgcc
     8521 gtcggcgagg gcgtggatcg ggcgtggccc ggcaccgctg tcgtcatcaa tcccagcctc
     8581 gagtgggggc ccgacagccg cgcccagggc ccgtcgttcc gcatcctcgg cctccccgac
     8641 cacgggacct acgcggagct cgtgaaggtg ccggccgcca atctgtaccg gaagcccgcg
     8701 tcgctctcgt gggaggccgc ggcggcgatt ccgctcgccg gcctcacggc gtaccgggcg
     8761 ctcgtcacgc gcgccaggct gcagccggga gagaccgtgc tcgtcaccgg cgtcggcgga
     8821 ggcgtggcca ccttcgtgct gctcttcgcg ctgcacctgg gtgcccgcgt ggtcgtcacc
     8881 tccgggagcg atgccaagat cgcccgggcg cgggaactcg gcgccgccgg cggcgcgaac
     8941 taccggctcg acggctgggg ccaggcgatc gcgaggctga ccgacggcgg ccccgacgtg
     9001 atcgtcgaca gcgcgggccg cgaggcgttt ccgacgctgc tggaggtcgt ccgcccgggc
     9061 gggcgcatcg tgacgttcgg cgccacgaca ggctcggcct ccacggtcga agtgcgccgg
     9121 gtcttctgga agcaggtcga catcctcggc agcacgatgg gcacgccgga ggagttctct
     9181 agcatgctgg cgctcttcga tcgcggcctc gagccggtga tcgaccggac gtttccgctg
     9241 gccgcggcgg ccgacgcgca ccggcgcatg gaccaggccg atcagttcgg caagctcgtc
     9301 ctgacgatcc agccgcaggc gtgaccttct tccttcggga agagcggcgt gcgcggatcg
     9361 gaagcgagac ggcggcggcg aagctcgagg ccgaacacga gcgccgcgcc tgctcgccgg
     9421 aatcggcagc ggcctggccg cgttcgagga gcgccccgcg cagtccgggg cttgccgggt
     9481 cagagagcct ggtcaccgag gtctgcgctg cggtcgcttc cgtacggcct gtctgcagat
     9541 tgcgcagcgc ggtgatgctg atcgggaggc cgaggacgga ccggcagcag ggcatcatcg
     9601 gcaacgcctg gcgatcgcgc agggtcccgg tcagcggctt cggcaggatc tcgaggacga
     9661 tcgcgccctc accggcccac cgccttgccc caccgggctt cgagcgcttc gagaatcgcc
     9721 tcggcatggt cttcgtcgat ccgcagcagc agcggctcgc cggcggtttc gaccgccaac
     9781 acgagccggg tgctcgacag gaggccaagg cgctttcgtt cgatcctcga gcaggacgcg
     9841 gcggcgatgt cccggtagcg cacgctccgg agggcggcgc cgccgccttc gggcgtgacg
     9901 cgcaagcggt cggaggcgaa gacgagaatc gcgccgaact cgcgcggccc gtcgtcggtg
     9961 cgggcgagat acttcacccc gcggaacgtc gggtcgggag acggggccga ggcggtcccg
    10021 gtctggcgtc cgggtgcggt gccggtgcgt ccctcgcccg acagcgccgc cgggcgcgct
    10081 gccggcagca tctcgagcaa ggcgccgcgc ttccagcaat acgcgcccgc cgccgccacg
    10141 cccagcgcca cgatcagcgc agcgacgaca ccggtgccca ggcgggcagg cgcgttcccg
    10201 gcgccctccc cgcgcggggc ctccgcccgc cccggagcgc cggcgcggaa tggaagaggc
    10261 acctgttcct gggcccggtg cgcgcccgac ggagtggcct gcgcccactc tccggatgac
    10321 actcgcgccg atggagagcc cgccagcgcc ggcgtgggac ccgcaagccg atatgcgccg
    10381 gccctcg
//
LOCUS       DAOSIF010000048        10253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6365_length_10253_cov_3.426260, whole genome shotgun sequence.
ACCESSION   DAOSIF010000048 DAOSIF010000000
VERSION     DAOSIF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6365_length_10253_cov_3.426260"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLT35_02980"
     CDS             complement(<1..366)
                     /locus_tag="PLT35_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28217.1"
                     /translation="MILAVHEDLRRRVTAAVTGLYGLAPTDLPQVPIAMPPNRGLGDL
                     AVTVAFDLARRLRKPPRAVGQELAARLGVVPGVARVECAANGYLNVFLDRPAFLRDRL
                     SNRVDGGGGAARDKVIVEHT"
     gene            complement(411..1025)
                     /locus_tag="PLT35_02985"
     CDS             complement(411..1025)
                     /locus_tag="PLT35_02985"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOG28218.1"
                     /translation="MESAAILAGGRASRMGGQDKGRLLVGARTILERELDALAGLGCP
                     VVIITPEPDRYADTGLAVIADALPGCGSLGGIYTAVTLGDGPVLVLACDMPFLTTAFL
                     ARLQEAGREADVAIPKTPAGYHPLCASYAPSCAPAIRSRIESGALKASGFLQDVRVRE
                     VGPGEIAPFDPDGLLLFNVNTPADLAAARAAARRLERAARRGPS"
     gene            1073..>1966
                     /locus_tag="PLT35_02990"
     CDS             1073..>1966
                     /locus_tag="PLT35_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28219.1"
                     /translation="MGTAVIAPFLATSLYQAISEALPARVWFYESWLTPRGFRAKHVY
                     VSGVQAIFSFLRHEHEQYDAVMRRAGSLSARWVHDGLTPFVRWPLHALPRALRARAAL
                     RLARHVVRRTWSESRVRIRWRGRVGTFAITRSLFCAVREAAPQPLCGFYAAVVETCLE
                     IAGVAGEVAIAGCLASGNGECVVRVTRTAARRPGADAGRAAAALVAMAGALAQATVAA
                     QAPAASGAAERAVTAQDVPAAAPRPLGSGGVLVMPFENASRQPGLAWLSEGSALVLAD
                     EFRRAGLEVMAREERRCAFDRL"
     assembly_gap    1967..1976
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1977..3393
                     /locus_tag="PLT35_02995"
     CDS             <1977..3393
                     /locus_tag="PLT35_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG28220.1"
                     /translation="RLQVPPRAALSRATVVRIGDLIGADEVVTGALMVEAGDLVLRAR
                     RIHLESGRMDAEQDVRGPLAELPGLYRELAARLLGAGPAAQPGQAEPAGLVLGVPPAA
                     FEQYVKGLIAESLAAQTTFLSKALDLHPEFPEARLALAQSRLAAGDYRLALDALGPIM
                     GSASHGAEAGLVAALAHIALGSFTPAYQVLAALQARAPSAPVLNNLGVVRLRAGSLPP
                     GAGSAVWYFDQARRLEPLEPDYLFNLGYAHWLDGSPQGAGYWLREAVRLAPTDSAAHA
                     LLARALAGTGQPAEAARELALAGRLSSAFEGIDLQGGWPPRPPGGLERLKDSMAPRRA
                     RRIDAAIESAASRDQRDLAAFYLERGRRAFERESDRDAESDLRRALYLSPYEAEAHLL
                     LGRSYLRTGRLQEAIDAFKVSIWSRDTAAARLALAEAYLEARDPAACRAEAERALALD
                     PGSVHARQLLDQVRPPSAPPV"
     gene            3434..4807
                     /locus_tag="PLT35_03000"
     CDS             3434..4807
                     /locus_tag="PLT35_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOG28221.1"
                     /translation="MASCSDARVRRRRCARARRAIVLAMALGAAALTAPARADEAAPL
                     VFVAGVDALIHPVSAEFMVGAMERADAEEAALLVFVLETPGGLVDSTRTIVTRMLSSK
                     TPIAVFVAPSGARAASAGFLIIIAADVAAMAPGTHIGAAHPVSGQGEKMDETTSKKAA
                     SDVAAYARTLAAGRNRNVDWAEQAVRESRAFTETEALKASPPLIDLVASDVNDLLAQI
                     DGREVRRFDGRTQTLRTSGARIETITMPLRQRVLSALAHPNIAYILLSLGVLGLTVEL
                     WSPGAVLPGVVGGICLLLAFFTFQVLPVNYAGVLLILFGLALLVLEVKVASFGLLGVG
                     GIVSLLLGSMILIDAPTPELRVSLGLILPIVAALAAIVLFLVRLAVISQLRPSVTGAA
                     GMLGETGKALTPIPAGGEGRVSVHGEIWAARSAEAVGAGDTVVIGGIDGLVLSVARRP
                     SQGDPSS"
     gene            4804..5556
                     /locus_tag="PLT35_03005"
     CDS             4804..5556
                     /locus_tag="PLT35_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOG28222.1"
                     /translation="MTPNPLMLVAVIIAVYLVGSIKILAEYERGVIFRLGKLLPQPKG
                     PGVILVFAPIDRMVRVGLRTVVLDVPPQDIITRDNVSVKVNAVVYFRVVHPQNAIVQV
                     ENYHYATSQLAQTTLRSVLGQVELDDLLSARERLNAQLQQILDQHTDVWGIKVSAVEV
                     KHVDLPENMQRAMARQAEAEREKRAKIIHAEGELAAAEELAQAARIIGTQAETMTLRY
                     LQTLTEIAAEKNSTIVFPLPLDLLRGLFTRQS"
     gene            5581..>5891
                     /locus_tag="PLT35_03010"
     CDS             5581..>5891
                     /locus_tag="PLT35_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28223.1"
                     /translation="MSDERFRGVQLNDKQLVFAFMAATVVLVAVFLCGVLVGRGVRTA
                     RGPAQDDGTIASSQVVADAGAAEAPLADGADTPVRESGADAGATGFTYPARLGSHPPV
                     ER"
     assembly_gap    5892..5901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5902..6309
                     /locus_tag="PLT35_03015"
     CDS             <5902..6309
                     /locus_tag="PLT35_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG28224.1"
                     /translation="EVPAPDLPRARPEPPPDVPAEPVAEPSPSAADAPGPTRPPGAAG
                     AGAPNAAAPDAGTPGAYTVQVAAVRNRAEADAIVRQLKAKGFDARVLAPGARDNPAVF
                     RVRIGSFKDKRAADALAERLKREEKRYQPWVTR"
     gene            6330..7817
                     /gene="lnt"
                     /locus_tag="PLT35_03020"
     CDS             6330..7817
                     /gene="lnt"
                     /locus_tag="PLT35_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOG28225.1"
                     /translation="MALSFPRYGHPALAWAALAPLLVALAGHGGRPPRRPFLLGLLSG
                     TVYFAGTLYWTPDVLRTFGGVSAPLAVFAGALLVAYLALFPAGFAAVTARVAARSGPP
                     GLMIAPAAWVATEWLRRWILGGFPWVQLGSSQAGVIPVVQAASLAGVYGLSALLAATS
                     ASVAVGIVGRGRVRWAAPAGMALLVGALVAWGAWRVREGSLSAGGPTIRVALAQGNIP
                     QHQKWDARQAERILGTYLDQTRDAAARGASLVVWPESSTPFMFEHDRAGQDAIRRTVR
                     EAGVSLLFGSDQFEAGKPPRFYNAAFLLAPDGSTAGVYRKMHLVPFGEFVPLKRLLFF
                     VAPLVESVSDFSPGRQAVVMPIGANRLSTAICYEVVYPDLIAAFTARGSQLLTTITND
                     AWYGRSSAPYQHFWMATLRAVEQGRYLLRAANTGISGIVDPYGRVTAMTPLFEPAVVT
                     GEVRFLDHRTVYARTGDAFAWACAVGALAAWFVARPHKTRSRHRR"
     gene            7861..8995
                     /gene="prfB"
                     /locus_tag="PLT35_03025"
     CDS             join(7861..7938,7940..8995)
                     /gene="prfB"
                     /locus_tag="PLT35_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110174822.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOG28226.1"
                     /translation="MAFEADDLIRRHDDLTERSAELRRYLEAGKPRDEASRLEALIAQ
                     PDFWKDQAEARRILQRRRRLQDDIDLTESLRTQEADLAVLAEWAASGEDVLSDLASGL
                     DRLEEQVEVAETKRILGGEHDRASAVMTIHPGAGGTESQDWADMLLRMYLKWAERRGF
                     KREILDYQPGDEAGLKSATVTVTGEYAYGLLLAEAGVHRLVRISPFDQDARRHTSFAS
                     VYVWPEIEDDTEIEVDEKDIRVDTFCSSGPGGQGVNTTYSAVRITHLPTSIVVSCQNE
                     RSQIRNREAAMKVLKSRLYDIKIREQQAEIERLGGEKKDIAFGSQIRSYVLQPYQMVK
                     DHRTKVQMGDVERVLGGDIDVFIKSYLRAKANGTLGQATGADD"
     gene            complement(9011..>10253)
                     /locus_tag="PLT35_03030"
     CDS             complement(9011..>10253)
                     /locus_tag="PLT35_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG28227.1"
                     /translation="APRPAARGSAEDPAKQALPPGAARVRVGKYVGILDRQSVQWTGR
                     QSAADLVKPGDLIEVGITSLDEAAGTAKLSLEQTPIVEGALLAIENRTGQIRAMVGGF
                     DFGRSKFNRAVQAPRQIGSAFKPIVFTAAIDRGYTPSSIIVDEPVSYFAGPGQPLYQP
                     QNYDRKYEGAITLRRVLEQSRNVPTVKLMDQLTPQQVIGYARRLGFESPLQPYLSTAL
                     GASEATLLEVTSAYSAFANQGVRMVPYSIVQVSERDGTVLEEQHPVAREGIRADTAFV
                     VANMLRGVVQRGTAAKAAELNWPLAGKTGTVDDYTDAWFVGFDPDITVGVWIGFDLKK
                     SLGRNETGAVAALPVWIAFMREYIDRRQDRAGPPAFQPPDNIVFVAVDRATGAMADPA
                     DPSAITEAYISGTQPGIGFPR"
BASE COUNT         1393 a         3643 c         3743 g         1454 t
ORIGIN      
        1 ggtgtgctcg acgatgacct tgtcgcgggc cgcgccgccg ccgccgtcca cgcggttcga
       61 gaggcgatcg cgcaggaagg ccgggcggtc caggaacacg ttgaggtagc cgttcgccgc
      121 gcactcgacg cgggccacgc ccggcaccac gccgagccgc gccgccagct cctggccgac
      181 ggcgcgcggc ggcttcctca agcggcgcgc gagatcgaag gcgacggtca cggccaggtc
      241 gccgaggccg cggttcgggg gcatcgcgat tggcacctgc ggcaggtcgg tcggcgcgag
      301 gccgtacaag cccgtgacgg cggctgtcac gcggcggcgg aggtcttcgt ggacggcaag
      361 gatcatggtg tgctcttccg gcggccgttg actcggcttc ggccagccat ctacgatggg
      421 ccgcggcgcg ccgcgcgctc gaggcggcgg gccgcggccc gggccgccgc caggtccgcg
      481 ggcgtgttga cgttgaacag gagcaggccg tccggatcga acggcgcgat ctcgcccggc
      541 cccacctcgc gcacgcggac gtcctggaga aacccgctcg ccttgagcgc gcccgactcg
      601 attctggagc ggatggccgg agcgcacgac ggggcgtagc tcgcgcacaa cgggtgatac
      661 ccggctggcg tcttcgggat ggcgacgtcg gcctcccgcc cggcctcctg gagccgggcg
      721 aggaacgccg tcgtcagaaa cggcatgtcg caggccagga cgaggacagg cccgtccccg
      781 agcgtcacgg cggtgtagat gccgccgagg ctgccgcagc cgggcagcgc gtcggcgatc
      841 acggccagac cggtgtccgc atagcgatcc ggctccggcg tgatgatgac gaccggacag
      901 ccgaggccgg cgagcgcgtc gagttcgcgc tcgaggatgg tgcgggctcc cacgaggagg
      961 cggcctttgt cctgcccgcc catgcggctg gcccggccgc cggcgaggat cgctgcgctc
     1021 tccatgaatc gatgcgcggc tccacgcgga aatacagtaa gttagcagaa aaatgggtac
     1081 ggcggtaatc gctccattcc tcgcgacgag cctctaccag gcgatctcgg aagcgctccc
     1141 ggcgcgggtc tggttctacg agtcatggct gaccccgagg gggtttcggg ccaagcacgt
     1201 ctacgtctcc ggcgtccagg cgatcttcag cttcctgagg cacgaacacg aacagtacga
     1261 cgcggtcatg cgccgcgccg gatcgctttc ggcgcgctgg gtgcacgacg ggctgacgcc
     1321 gttcgtgcgg tggccgctcc acgcgctgcc gcgcgcgctc agggcgcgcg cagcgcttcg
     1381 cctcgccagg cacgtcgtgc ggcgcacgtg gagcgaaagc cgagtgcgga tccgctggcg
     1441 cggcagggtg ggcacgttcg ccatcacgcg gtcgctcttc tgcgccgtcc gggaggcggc
     1501 gccgcagccg ctatgcggct tctatgctgc cgtcgtcgag acctgcctgg agatcgcggg
     1561 cgtcgccggc gaggtggcca tcgccggctg cctggcgagc ggaaacgggg agtgcgtggt
     1621 gagggtcacc cgcacggccg cgcgcaggcc gggcgccgat gccggacggg ccgcagccgc
     1681 gctcgtcgcg atggcggggg cgctggcgca ggcgaccgtt gcggcgcagg cgccggcggc
     1741 gtccggggcg gccgagcgag ccgtgacggc gcaggacgtc ccggcggccg cgcctcggcc
     1801 gctcggcagc ggaggcgtgc tcgtgatgcc gttcgagaac gcctccaggc agcccggcct
     1861 cgcgtggctg tcggaaggat cggctctcgt cctggccgac gagttccgcc gagccggact
     1921 cgaggtcatg gcgcgcgagg aaaggcggtg cgcgttcgac cgcctcnnnn nnnnnnccgc
     1981 ctccaggtgc cgccgcgggc cgccctcagc cgggcgaccg tcgtgcggat cggcgatctg
     2041 attggcgccg acgaggtcgt gacgggcgcg ctgatggtcg aagccgggga cctcgtgctg
     2101 cgcgcgcggc ggatccacct cgagtcgggg cggatggacg cggaacagga cgtgcgcggc
     2161 ccactggcgg aactgcccgg gctctaccgg gaattggccg cgcgcttgct cggggcgggg
     2221 ccggcggcgc agcccggcca ggcggagccg gcggggctcg tcctcggcgt gccgcctgcg
     2281 gcgttcgagc agtacgtgaa gggcctgatc gccgagtcgc tggccgccca gacgacgttc
     2341 ttgtccaagg cgctcgattt gcaccccgag ttcccggaag cccgcctcgc gctcgcccag
     2401 tcgcggctgg ccgccggcga ctaccggctc gcactcgacg cgctggggcc gatcatgggc
     2461 tccgcctccc acggcgccga ggcgggcctg gtcgcggccc tggcgcacat cgccctcggc
     2521 agcttcacgc ccgcctatca ggtgctggcc gcgctccagg cgcgcgcgcc gtccgcgccg
     2581 gtgctgaaca acctcggcgt ggtgcgcctg cgcgccggat cgctgccgcc cggcgcgggg
     2641 agcgcggtgt ggtacttcga ccaggcgcgc aggctggaac ccctcgaacc ggactatctc
     2701 ttcaacctgg ggtacgctca ctggctggac gggagcccgc agggggccgg atactggctg
     2761 cgggaagccg tgcggctcgc gccgaccgac agcgcggcgc acgcgctgct ggcgcgggcg
     2821 ctggcgggga cagggcagcc ggccgaggcc gccagggaac tcgcgctggc cgggcgcctg
     2881 tcgtcggcgt tcgaagggat cgacctccag ggcgggtggc ccccccggcc cccgggcggg
     2941 ctcgaacggc tcaaggactc gatggcgccg cggcgggcgc gccgcatcga cgcggcgatc
     3001 gaatccgctg ccagccggga ccagcgcgac ctggcggcgt tctacctcga gcgggggcgg
     3061 cgcgcgttcg agcgcgagag cgatcgggac gccgagtccg acctgcgacg cgcgctctac
     3121 ctgtcgccgt acgaggcgga ggcgcacctc ctgctcgggc gctcgtactt gcggacgggc
     3181 cggctgcagg aggccatcga cgcgttcaag gtgtcgatct ggagccggga cacggcggcc
     3241 gcgaggctcg ccctcgccga ggcctacctc gaagcgcgcg accccgccgc atgccgggcg
     3301 gaagccgagc gcgcgctggc cctcgacccg ggttcggtcc acgccagaca gctcctggac
     3361 caggtccgtc ccccatcagc ccctccggta taatcgtgct gcgcccgccg gagcgggcgc
     3421 ggagggccgc gcgatggcta gctgttccga cgctcgggtg cggcgccgcc gatgcgcccg
     3481 cgcccgccgt gcgatcgtgc tcgcgatggc cttgggcgcg gcggcgctga cggcaccggc
     3541 ccgcgccgat gaggccgcgc ccctcgtctt cgtcgccggc gtcgacgccc tcatccatcc
     3601 cgtgtcggcc gagttcatgg ttggcgccat ggagcgcgct gacgcggaag aggcggcgct
     3661 cctggtcttc gtgctcgaga cgcccggcgg cctggtggat tccacgcgca ccatcgtgac
     3721 gcggatgctc tcgtcgaaga cccccatcgc cgtcttcgtg gcgccgtcgg gcgcgcgggc
     3781 agcctcggcg gggttcctca tcatcatcgc cgcggacgtg gcggcgatgg cccccggcac
     3841 ccacatcggg gccgcccacc cggtgtcggg gcagggcgag aaaatggacg aaacgacgtc
     3901 caagaaagcc gcttccgacg tggccgcgta cgcccgcacg ctcgcggccg gccggaaccg
     3961 caacgtcgac tgggcggaac aggcggtccg cgagagccgc gccttcaccg agaccgaggc
     4021 gctcaaggcc tcgccgcccc tcatcgacct ggttgcctcc gacgtgaacg acctgctggc
     4081 ccagatcgac gggcgcgagg tccgccggtt cgacgggcgg acgcagacgc tgcggacctc
     4141 cggcgcgcgc atcgagacga tcacgatgcc gctccgccag cgcgtcctga gcgcgctggc
     4201 ccacccgaac atcgcctaca tcctgctcag cctgggcgtc ctcgggctca cggtcgagct
     4261 ctggtcgccg ggcgcggtcc tgcccggggt cgtcggggga atctgcctgc tgctggcgtt
     4321 cttcaccttc caggtgctgc ccgtgaacta cgccggcgtg ctcctcatcc tcttcggcct
     4381 cgcgctgctc gtcctcgagg tcaaggtcgc cagcttcggc ctgctcggcg tcgggggcat
     4441 cgtcagcctg ctgctgggct cgatgattct catcgacgcg cccacgccgg agctgcgggt
     4501 gagcctcggc ctcatcctgc cgattgtcgc cgcgctcgcg gcgatcgtcc tgttcctggt
     4561 gcgcctcgcc gtgatctcgc agctccgtcc ctcggtgacg ggcgcggcgg gcatgctggg
     4621 cgagacgggg aaggcgctga cccccattcc cgccggcggg gaaggccggg tgtccgtgca
     4681 cggggagatc tgggccgcgc gctccgccga ggccgtcggc gcgggagaca cggtcgtcat
     4741 cggcggcatc gacgggctcg tcctgtccgt cgcgcgccgc ccatctcagg gggatccgtc
     4801 atcatgacgc ccaatccgct catgctcgtc gccgtcatca tcgcggtgta cctggtcggg
     4861 tcgatcaaga tcctcgcgga gtacgagcgc ggcgtgatct tcaggctggg caagctgctg
     4921 ccccagccga agggcccggg tgtgatcctc gtcttcgcgc ccatcgaccg catggtccgg
     4981 gtcggcctgc gcacggtcgt gctggacgtg ccgccgcagg acatcatcac gcgggacaac
     5041 gtctcggtga aggtcaacgc ggtcgtgtac ttccgcgtcg tccatccgca gaacgccatc
     5101 gtccaggtgg agaactatca ctacgccacg tcgcagctgg cccagacaac gctgcggagc
     5161 gtgctcggac aggtggaact cgacgacctc ctctccgcgc gggagcggct gaacgcgcag
     5221 ctccagcaga tcctggacca gcacacggac gtgtggggca tcaaggtgtc ggcggtcgag
     5281 gtcaagcacg tcgatctgcc ggagaacatg cagcgcgcca tggcgcgcca ggcggaagcc
     5341 gagcgcgaga agcgcgcgaa gatcatccac gccgagggcg agctcgccgc cgccgaggaa
     5401 ctcgcgcagg cggcgcggat catcggcacg caggcggaga cgatgacgct gcggtacctc
     5461 cagacgctga ccgagatcgc ggcggagaag aactcgacga tcgtgttccc gctgcccctc
     5521 gacctgctgc gcgggctgtt cacccgccag tcgtagcggg ccgccgaggc gcgggcgctc
     5581 atgtccgacg aacggttccg cggcgtgcag ctgaacgaca agcagctcgt gttcgcgttc
     5641 atggcggcca ccgtcgtgct ggtggccgtc ttcctgtgcg gggtgctcgt cggccggggc
     5701 gtgcggaccg cgcggggccc ggcacaggat gacggaacga tcgcgtcgtc gcaggtcgtg
     5761 gccgatgccg gcgccgccga ggcgccgctg gcggacggcg ccgacacgcc cgtccgcgaa
     5821 tccggcgcgg acgcaggcgc caccggcttc acctacccgg cgcggctggg atcgcatccg
     5881 ccagtggagc gnnnnnnnnn ngaggtgccg gccccggacc tgccgcgggc gaggccggag
     5941 ccgcctcccg acgtgccagc cgaacccgtc gcggagccga gcccatccgc cgccgacgcg
     6001 cccgggccga cgcggccgcc gggggcggct ggggcgggcg cgccgaacgc ggccgcgccc
     6061 gacgccggga ctccaggcgc gtacaccgtg caggtggccg ccgtgcggaa ccgcgccgag
     6121 gcagacgcga tcgtccggca gctgaaggcc aaggggttcg atgcgcgggt cctcgctccg
     6181 ggcgccaggg acaaccccgc cgtgttccgc gtgcggattg gctcgttcaa ggacaagcgc
     6241 gccgccgacg ctctcgccga gcgcctgaag cgcgaagaga agcggtacca gccctgggtc
     6301 acgcgttagc gctcctctcc ggcgtcctgc tggccctcag cttcccgcgg tacgggcatc
     6361 ccgccctggc gtgggccgcc ttggcgccgc tcctggtggc cctcgccgga cacggcgggc
     6421 ggccgcctcg ccgcccgttt ctgctcggcc tcctttccgg taccgtctac ttcgcgggca
     6481 cgctgtactg gacgccggac gtgctgcgga ccttcggggg cgtcagcgcg cccctggcgg
     6541 tgttcgccgg cgcgctgctc gtggcctacc tggcgctctt cccggccggg ttcgccgccg
     6601 tgaccgcgcg cgtcgccgcg cgcagcggcc cgccggggct gatgatcgcg cccgccgcgt
     6661 gggtggcgac cgagtggctg cgccgctgga tcctcggggg gttcccgtgg gtgcagcttg
     6721 ggtcgagcca ggccggcgtc atcccggtcg tgcaggccgc aagcctggcc ggcgtctacg
     6781 ggctgtcggc gctcctggcg gcaacctcgg cctccgtcgc cgtgggcatc gtcgggcgcg
     6841 ggcgggtccg ctgggcggcc ccggcaggga tggcattgct cgtcggcgcg ctcgtcgcct
     6901 ggggcgcgtg gcgcgtgcgg gagggaagcc tgagcgcggg cggcccgacc atccgcgtgg
     6961 cgctcgcgca gggcaacatc cctcagcacc agaagtggga cgcgcggcag gccgagcgca
     7021 tcctcggcac ctatctcgac cagacccgcg atgcggctgc ccgcggagcg tcgctcgtcg
     7081 tgtggcccga gtcctcgacc ccgttcatgt tcgagcacga tcgggcgggg caggacgcca
     7141 tccgccggac ggtgcgcgag gcgggcgtgt cgctcctgtt cgggagcgac cagttcgagg
     7201 cggggaagcc gccgcgcttc tacaacgccg cgttcctcct cgcgcccgac ggcagcaccg
     7261 ccggcgtgta ccgcaagatg cacctcgtgc ccttcggcga gttcgtgccg ctgaagcgcc
     7321 tgctcttctt cgtggcgccg cttgtcgagt cggtgtccga cttctcgccc ggccgccagg
     7381 ccgtcgtgat gccgattggc gcgaacaggc tctcgacggc gatctgctac gaggtggtgt
     7441 atccggacct catcgcggcg ttcaccgcgc gcgggagcca gctgctgacg acgatcacga
     7501 acgacgcctg gtacgggcgg tcgtcggcgc cgtaccagca cttctggatg gccacgctcc
     7561 gggccgtcga gcagggccgc tacctgctgc gggccgccaa cacgggcatc agcggcatcg
     7621 tcgatccgta cgggagggtc acggcgatga cgccgctctt cgaaccggcg gtcgtgaccg
     7681 gcgaggtgcg gttcctcgac caccgcacgg tgtacgcccg caccggcgac gccttcgcct
     7741 gggcgtgcgc cgtcggcgcg ctggcggcgt ggttcgtcgc gcgcccgcac aagacgcgca
     7801 gccgccatcg gcgctagaat cgagctaaga cggcatcgac gccgtccagg agaccgcgca
     7861 gtggcattcg aggcggacga tctgatccgg cggcacgacg acctcaccga gcgatcggcg
     7921 gaactgcgga ggtacctttg aggccgggaa gccgcgcgac gaggcctcgc gcctcgaagc
     7981 cctcatcgcc cagccggact tctggaagga tcaggccgag gcccggcgca tcctgcagcg
     8041 ccggcgccgc ctccaggatg acatcgacct gaccgagtcc ctgcggacac aggaggccga
     8101 cctggcggtc ctcgccgagt gggcggcgtc gggcgaggac gtcctgtccg acctggcctc
     8161 gggcctcgac cggctcgaag agcaggtcga ggtcgcggag accaagcgca tcctgggagg
     8221 cgagcacgat cgggccagtg ccgtgatgac gatccatccc ggggctggcg ggaccgaatc
     8281 gcaggactgg gccgacatgc tgctgcgcat gtacctcaag tgggcggagc ggcgaggctt
     8341 caagcgcgag atcctggact accagccggg cgacgaggcc gggctcaaga gcgcgacggt
     8401 caccgtgacg ggggagtacg cctacggcct gctgctcgca gaagccggcg tgcaccgcct
     8461 cgtccgcatc tcgcccttcg accaggacgc ccggcggcac acgtccttcg cctcggtcta
     8521 cgtctggccc gagatcgagg acgacacgga gatcgaggtc gacgagaagg acatccgggt
     8581 ggacaccttc tgctcgagcg gcccgggcgg gcagggcgtg aacacgacgt actcggccgt
     8641 ccgcatcacc cacctgccga caagcatcgt cgtgtcgtgc cagaacgagc ggtcgcagat
     8701 ccgcaaccgc gaggcggcga tgaaggtgct gaagtcgcgc ctctacgaca tcaagatccg
     8761 ggagcagcag gcggagatcg agaggctcgg cggcgagaag aaggacatcg ccttcggcag
     8821 ccagatccgc agctacgtgc tgcagccata ccagatggtc aaggaccacc gcaccaaggt
     8881 gcagatgggc gacgtcgaac gggtgctcgg cggcgacatc gacgtcttca tcaagtccta
     8941 cctgagagcg aaggccaacg gcacgctggg ccaggcaacg ggggccgacg actaggaagc
     9001 ccgggccccc ttaccgcggg aacccaattc ccggctgcgt gccggagatg taggcctcgg
     9061 taatggccga cggatccgcc gggtcggcca tcgcgccggt ggcgcgatcc accgccacga
     9121 acacgatgtt gtcgggcggc tggaaggccg gcgggccggc ccggtcctgg cgccgatcga
     9181 tgtactcgcg catgaacgcg atccagacgg gcagggccgc cacggccccg gtttcgttcc
     9241 ggccgaggga cttcttcaag tcgaagccta tccagacgcc gaccgtgatg tccgggtcga
     9301 atcccacgaa ccaggcatcc gtgtaatcgt ccaccgtccc ggtcttgccg gccagcggcc
     9361 agttcagctc ggcggccttc gccgcggtgc cgcgctgcac cacgccgcgg agcatgttgg
     9421 ccacgacgaa ggcggtgtcc gcccggatgc cttccctggc gacagggtgc tgctcttcga
     9481 gcaccgtccc gtcccgctcc gacacctgca cgatgctgta cggcaccatg cggacgcctt
     9541 ggttcgcgaa cgcggagtaa gcgctggtga cctcgagcag cgtcgcttcc gacgcgccga
     9601 gggcggtgga caggtacggc tgcagcggcg actcgaagcc cagccggcgc gcgtagccga
     9661 tgacctgctg cggcgtaagc tgatccatca gcttcaccgt cggcacgttc cgcgactgct
     9721 cgaggacgcg ccgcagcgtg atcgccccct cgtacttgcg gtcgtaattc tgcggctggt
     9781 agagcggctg cccgggcccg gcgaagtacg acaccggctc gtccacgatg atcgaggacg
     9841 gcgtgtagcc tcgatcgatg gccgcggtga agacgatcgg cttgaaagcg gatccaatct
     9901 gcctcggcgc ctggaccgcg cggttgaact tgctccggcc gaagtcgaag ccgcccacca
     9961 tggcccggat ctggccggtc cggttctcga tggccagcag ggccccctcg acgatgggcg
    10021 tttgctcgag cgacagcttg gcggtgccgg cggcctcgtc gagcgacgtg atgccgactt
    10081 cgatgagatc gcctggcttc acgaggtcag cggccgactg ccggcccgtc cactggacgc
    10141 tctgccggtc gagaatgccg acatacttgc cgacgcgaac gcgggccgcc ccgggcggca
    10201 gcgcctgctt cgccggatcc tccgcggatc ctctcgccgc gggccgcggc gcg
//
LOCUS       DAOSIF010000049        10209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6396_length_10209_cov_4.823813, whole genome shotgun sequence.
ACCESSION   DAOSIF010000049 DAOSIF010000000
VERSION     DAOSIF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6396_length_10209_cov_4.823813"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLT35_03035"
     CDS             <1..714
                     /locus_tag="PLT35_03035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG28228.1"
                     /translation="WHTTNDLYSELVPYAEHQQHSALVTAVVAYGVANLEKPLTRAGV
                     YLADGLYATLAIGSGEETRQIMTTLDYVNAPLATANFVRIVEGKAPAPAGRAGGPPPG
                     MGFGGRGGRGGPEQKPIGQVLDVKDGLVSMQIVSDTQKSVAVARLPRTLNPKLGHDAA
                     GVLGLSGPNVFYLTLQKKSSLNRRSAALGTVIAGLSHLQDLKKGDTIRAVRITRVGDG
                     ARDFKTDDEAFAKLLGAKK"
     gene            complement(1048..1803)
                     /locus_tag="PLT35_03040"
     CDS             complement(1048..1803)
                     /locus_tag="PLT35_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOG28229.1"
                     /translation="MNRTARHLRIREILERQEFVDLQALCRLLDASESTVRRDLADLE
                     RLQALKRVHGGALGVQPRDHLLDHDWQARRQSEEKRRIGRLAATLVADGETVILDGGS
                     TVACVADELANRPIHAITNSLAIARLFRAPCSVEVTLTGGYLFPRLQVMLGPLCERML
                     ASVAADAVIMGIGGVTEAGFSNNHTLVVGSELRMIEVARRVIIVADATKFGRPAMVPL
                     APLDEADIVVSDASLPPEHRRMLEDRGVKVLLA"
     gene            complement(1800..2900)
                     /locus_tag="PLT35_03045"
     CDS             complement(1800..2900)
                     /locus_tag="PLT35_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOG28230.1"
                     /translation="MRPMDLTAYGVIALADVEAAAREGAREILTRERVVLTPSAREAA
                     ERHGIVFRAVGSGASAAASAQPPAPQAAAPPPPSRAVRDLFTSPEAEAVKAEIVRVGR
                     KLWHRQYVDGNGGNISYRIGPNEVICTPTLRSKADLAPEDLCLVDLEGNELLRGPYPR
                     TSEIFLHLEIYKAQPKAKGVVHCHPPHATAYALVGRVPPSGAVPEFDVFVGKVACTQY
                     ETPGTKAFAETVLPFVLNYNTVLLGNHGIVCWADTVTHAEWYAEVLETYCWTLTIASQ
                     LGVPFSKIPAEKEQDLLKIRQRLGLPDPRFNPEACRAAEIAEGEPGEIAVAPPPACVY
                     EPARPGQADIESLVRDVTSAVMAALRERGLTA"
     gene            complement(2897..3223)
                     /locus_tag="PLT35_03050"
     CDS             complement(2897..3223)
                     /locus_tag="PLT35_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOG28231.1"
                     /translation="MRLGIVRGQVTLGLADAALAGTTLLIVDPVTAENLEARNGQGGG
                     RQIVVADRLSPGQGELIAFVEGAEAANAYFPGVAPVDAYCSLVVRDYEFKSPAAGTPP
                     GTEGPR"
     gene            complement(3220..3510)
                     /locus_tag="PLT35_03055"
     CDS             complement(3220..3510)
                     /locus_tag="PLT35_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOG28232.1"
                     /translation="MFLARIDGTLTSTRRHGTFEAVRFLIARRMDANGCMSGEPLVVL
                     DRMGAGPGTIALVSSDGKLARDWLGPAVPARFTVVGLVDRVHLPGRACGGPS"
     gene            complement(3520..5070)
                     /locus_tag="PLT35_03060"
     CDS             complement(3520..5070)
                     /locus_tag="PLT35_03060"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOG28233.1"
                     /translation="MIADRDRVVADIAQEVVARLLAYPGAAPAAVLRPAPGSRPPGGA
                     SVRPLAPAAPARAAAQPATPAAAKQKRAPLGDGVFATVDEAVKAAAAAQARVAQMSLD
                     ERGRMIGVIRRLCEERAEPLGRMEMDETRIGRLDHKIAKLRAMRLVLGVEAMRSDARS
                     DRSGLCVIERAPWGVIGMILPATHSVPTMASNAINVLAAGNTAVFSPHPAGALVAAHA
                     LALFNREIHREVGLTNVITTMAEASIEAAGEVFRHPDVALLCVTGGPAVVKAAAQSGK
                     RVIAGGPGNPPVVVDDSADLDLAARSIIAGAAFDNNLLCIGEKEVFVLESVADDFMAA
                     MERAGAVRLDARAIARLTEAAFTFDGPGRGCGRAHVKRDLLGQDASVLAEAAGVRVPA
                     GTELLFGETDASHAFVAEEQMMPFLPIVRVPCIDAAIEAAVRAEQGYRHTAVIHTRDV
                     EHATRMARAVNTTLFIHNAASLAALGIGGPGYFSHTIATPTGEGITTPLTFTRERQIA
                     VGGALRIV"
     gene            complement(5067..5366)
                     /locus_tag="PLT35_03065"
     CDS             complement(5067..5366)
                     /locus_tag="PLT35_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOG28234.1"
                     /translation="MLLARVIGSVVATQKHAKFQGAKLLVVQPCVAKDGVLTESGNTV
                     VAVDSVGAGLGTYVLFTQGSSARMTPMTKDSPTDAVIVGIVDSVEMGAATVEMPQ"
     gene            complement(5384..5656)
                     /locus_tag="PLT35_03070"
     CDS             complement(5384..5656)
                     /locus_tag="PLT35_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOG28235.1"
                     /translation="MAQTAIGMIETRGLTPLVQAADAALKAADVTFKGWKKVGSGMCT
                     IFLAGDVAAVKAAVDAGAVAARSVGEVVSVHVIPRPHDEVAGTLPG"
     gene            complement(5741..6007)
                     /locus_tag="PLT35_03075"
     CDS             complement(5741..6007)
                     /locus_tag="PLT35_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOG28236.1"
                     /translation="MEALGLIETKGLVGLVEASDAMCKAANVKLTARIQIGGGYVTSI
                     VRGDVGSVRAAVDAGAAAAKTVGELVAAHVITRPHDGLVAGMLP"
     gene            complement(6037..6318)
                     /locus_tag="PLT35_03080"
     CDS             complement(6037..6318)
                     /locus_tag="PLT35_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOG28237.1"
                     /translation="MIETRGLVALFEAVDAMLKAAGVTFRTWEPVGSGLVAAVVEGEV
                     AAVKAAVDAGAAAAKPLGEVVSVLVIPRPHDDLDGLVGRLGPKAAAGLS"
     gene            complement(6432..7136)
                     /gene="pduL"
                     /locus_tag="PLT35_03085"
     CDS             complement(6432..7136)
                     /gene="pduL"
                     /locus_tag="PLT35_03085"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: protein motif:HMM:NF011652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOG28238.1"
                     /translation="MAASTATIDRPPLTRAAIEEVVRQVVSARFGAAPAAGTNPLVVH
                     VSARHMHVSRADLDVLYGPGHELTPDRPLYQEGNYAAKETVTLVGRRGRSIPNLRILG
                     PLRKESQIELAFTDAILLGFDDVPVRLSGDIAGTPGAYVVGPAGMVQLKQGVIRAAIH
                     VHMNPAEAEHYGVKQGDLMRLRVESPAPLVFSRVVARIDPTSRLNVHMDTDEANACAL
                     HLATEFELSKSEGGGR"
     gene            complement(7126..8289)
                     /locus_tag="PLT35_03090"
     CDS             complement(7126..8289)
                     /locus_tag="PLT35_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28239.1"
                     /translation="MNILVANIGSTSFKYRLYAVERDTVLAQGRIERIGQTGGDCPDY
                     ETAIRRCVADIAGDGKPLARLDELAAIGFKAVHAGPVSGARVVDEDVLAAMEEFAFFA
                     PAHNPPYVAAMRSFARGVPGVPLVALFETAFFDRLDGAATTYAVPFDWREELGVRRYG
                     FHGASHRAANEHVQAHLGAGLRHISCHLGGSSSLAAVRDGVAADTSFGMSPQSGLPQS
                     NRCGDLDVFAALFVMKKRGLGPDEMARVLASQSGLAGISGLSGDIRDLDEAAASGNAR
                     ARLALDAFVRSIRHYLGAFLVTLGGVDVLTFSGGIGENSPDIRAGVCTGLGEFGIVLD
                     RGLNAAARGAARISAAASRVPILIVPADEERVVARATAEVVKGGGAAGGAHGR"
     gene            8506..9831
                     /locus_tag="PLT35_03095"
     CDS             8506..9831
                     /locus_tag="PLT35_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOG28240.1"
                     /translation="MNRSPKPSAPPTLDRIAELGVVGAGGGGFPAAVKLAARVSTVIV
                     NGAECEPLLHKDKELLQHLAEPMLRGLAAAMALTGAREGIIGIKEKYTAVMAGLEPLL
                     PPNVRILPLSDTYPAGDEFLLVHDAVGRIIPPGGLPKDVDAVVANVETLANIGLDRPV
                     THKYLTVAGAVAAPVTLAAPIGTSIGELIARAGGAAADRIAVLLGGAMMGRLAASLDE
                     PVTKTLGGLIVLPESHPLIERYKATPRQIARIGRSACDQCRFCTDLCPRFLIGHPIQP
                     HLAMQSLGFSTSPTSMAAGTLYCCECNLCSLYSCPENLDPKNVCVASKPAARERKLEW
                     KGTPDSIAAHPLAGDRRVPTKRLIAKLGLAGFRNVGPLEDLGAAPRRVVLPLKQHAGA
                     PAAPVVRPGDRVAAGDLVAAPAAGALGARIHASISGVVRSVDGAVVIEA"
     gene            9852..>10209
                     /locus_tag="PLT35_03100"
     CDS             9852..>10209
                     /locus_tag="PLT35_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOG28241.1"
                     /translation="MPKLKSIGLVEVSSIAAGYVALDAMLKAAQVDLLLARTICSGKY
                     LIVVGGDVASVQASVEAGAAKCDGSLIERRVVTKVHPAVFPAIGLAVDVTPEYTKAIG
                     LIETFSASSIVDVADAA"
BASE COUNT         1400 a         3722 c         3628 g         1459 t
ORIGIN      
        1 tggcacacga cgaatgacct gtacagcgaa ctcgtgccct acgcggagca ccagcagcat
       61 tccgcgctcg tcacggccgt cgtcgcgtac ggcgtggcga atctggagaa gcccctgacg
      121 cgcgcgggcg tttacctggc cgacgggctc tacgccacgc tcgcgattgg atccggcgaa
      181 gagacgcggc agatcatgac gacgctcgac tacgtcaacg cgccgctcgc gacggccaac
      241 ttcgtccgaa tcgtcgaagg gaaggccccg gcgccggccg gccgcgcagg cgggcctcct
      301 ccgggcatgg gcttcggcgg acgcggcggc cggggcggcc ccgaacagaa gccgatcggc
      361 caggtgctcg acgtcaagga cggcctggtc tcgatgcaga tcgtgtcgga cacgcagaag
      421 tcggtggccg tcgccaggct gccgcggacg ttgaacccga agctggggca cgacgccgca
      481 ggcgtgctcg ggttgtccgg gccgaacgtc ttctacttga cgctgcagaa gaagagcagc
      541 ctcaaccggc ggtccgcggc cctcggcacc gtgatcgccg ggctgtcgca cctgcaggat
      601 ctgaagaagg gcgacacgat ccgcgccgtc cgcatcacgc gggtcgggga cggcgcgcgc
      661 gacttcaaga ccgacgacga ggcgttcgcg aagctgctgg gcgcgaagaa gtagccggct
      721 ggcgggggga accaggcggc cgaaggcccg gcccgtggtt ccccgtgccg cccgttccag
      781 tcggcgcggc gccgtgagcc gccccgggcc ctctcagacg acgaccgccg gcaggacgtc
      841 cgacgcgtcc cggggccgcg atcccgccgg gccggaacgc ggtgcggctc gccacgccga
      901 atgtcgagcc ggccggcaac gcgctcggcc ggccgagggg cctggggcag ggcatcgcga
      961 cggtcaaccc ggcgccgccg ggcttcagga cgagcatcgg gtcgagcaag ggccgcgacg
     1021 cccggccggc gccgctcgtt cggcgaatca ggcgaggagg accttgacgc cccggtcctc
     1081 gagcatccgc cggtgctccg gcggcagcga cgcgtccgac acgacgatgt cggcctcgtc
     1141 gagcggggcc agcggcacca tcgccggccg cccgaacttg gtcgcgtccg ccacgatgat
     1201 gacgcggcgc gccacctcga tcatccgcag ctcggatccg accacgagcg tgtggttgtt
     1261 gctgaagccg gcctcggtga cgccgccgat ccccatgatg acggcgtcgg ccgccacgct
     1321 cgcgagcatc cgctcgcaca agggcccgag catcacctgg agcctcggga agaggtagcc
     1381 gcccgtcagg gtgacctcga cgctgcacgg ggccctgaac aggcgggcga tggccaggga
     1441 gttggtgatg gcgtggatcg gccggttggc cagctcgtcg gccacgcacg ccacggtgga
     1501 tccgccgtcc aggatcaccg tctcgccgtc ggctacaagc gtcgcggcca gccgtccgat
     1561 gcggcgcttc tcttcggact gccggcgggc ctgccagtcg tgatcgagca ggtggtcgcg
     1621 cggctggacg cccagggcgc cgccgtggac gcgcttcagc gcctgcagcc gctccaggtc
     1681 cgcgagatcg cgccggacgg tcgattccga cgcgtcgagc agccggcaca gtgcctgcag
     1741 gtcgacgaac tcctggcgct ccaggatctc gcggatgcgc aggtgccggg cggtgcggtt
     1801 cacgccgtga gccctcgttc cctgagcgcg gccattacgg cgctcgtcac gtcgcgcacg
     1861 agggactcga tgtccgcctg gccgggccgc gccggctcgt acacgcacgc cggcgggggc
     1921 gcgacggcga tctcgccggg ctcgccttcc gcgatctccg ccgcccggca ggcctccgga
     1981 ttgaaccgcg ggtctggcag gccgagccgc tgcctgatct tgagcaggtc ctgctccttc
     2041 tcggcaggga tcttcgagaa gggcacgccg agctgcgacg cgatcgtcag cgtccagcag
     2101 taggtctcca gcacttccgc gtaccactcg gcgtgcgtca ccgtgtcggc ccagcagacg
     2161 atcccgtggt tgccgagcag gacggtgttg tagttcagca cgaagggcag caccgtctcg
     2221 gcgaaggcct tcgtcccggg ggtttcgtac tgcgtgcacg cgaccttgcc gacgaacacg
     2281 tcgaactcgg gaacagcgcc ggatggcggc acgcggccga ccagggcgta cgcggtggcg
     2341 tgcggcggat ggcagtgcac gacgcccttc gccttcggct gggccttgta gatctcgagg
     2401 tgcaggaaga tctcgctggt ccgcgggtac gggccgcgca gcagctcatt gccctcgaga
     2461 tcgacgaggc acaggtcttc cggcgccaga tccgccttgc tgcggagcgt cggcgtgcag
     2521 atgacctcgt tcggcccgat ccggtaggag atgttgccgc cgttgccgtc cacgtactgc
     2581 cggtgccaca gcttcctgcc caccctgacg atctcggcct tgacggcctc ggcctcgggc
     2641 gacgtgaaga ggtcgcggac ggccctgctc ggcggcggcg gcgccgcggc ctgcggcgcg
     2701 ggcggctggg cggacgcggc cgccgaggcg ccgctgccga ccgcgcggaa gacgatgccg
     2761 tgccgctccg ccgcttctcg tgccgacggc gtcaggacga cccgctcgcg ggtgaggatc
     2821 tcgcgcgcgc cctcgcgcgc ggcggcctcg acgtccgcca gcgcgatcac gccgtacgcg
     2881 gtcaggtcca tcggtctcat cgcggccctt ccgtgcccgg cggcgttccg gccgccggcg
     2941 acttgaactc gtagtcgcgg acgacgagcg aacagtacgc gtccacgggc gccacgcctg
     3001 ggaagtaggc attggccgcc tcggccccct cgacgaaggc gatcagctcg ccctggccgg
     3061 gcgacagccg atcggccacg acgatctgcc ggccgccgcc ctgcccgttg cgggcctcga
     3121 ggttctcggc cgtcaccgga tcgacgatga gcagcgtcgt tccggcgagc gccgcgtccg
     3181 ccaggccgag cgtcacctgc ccgcgcacga tgccgagcct catgacgggc ctccgcacgc
     3241 gcggcccggc aggtggacgc ggtcgacgag gcccactacg gtgaaccgcg ccggcacggc
     3301 cgggcccagc cagtcgcggg ccagcttgcc gtccgacgac accagcgcga tcgtgcccgg
     3361 cccggcgccc atgcggtcca gcacgacgag cggctcgccg ctcatgcagc cgttggcgtc
     3421 catgcgcctg gcgatcagga accgcaccgc ctcgaaggtc ccgtgccttc gtgtggacgt
     3481 cagcgtgccg tcgatgcgcg ccaggaacat gggccctcgt cagacgatgc gcagcgcgcc
     3541 gccgacggcg atctggcgct cccgcgtgaa ggtcagcggc gtggtgatgc cctcgccggt
     3601 cggcgtcgcg atggtgtggc tgaagtagcc cgggccgccg atgccgagcg ccgccagcga
     3661 cgccgcgttg tggatgaaga gcgtcgtgtt cacggcgcgc gccatgcgcg tcgcgtgctc
     3721 gacgtcgcgc gtgtgaatga cggccgtgtg ccggtagccc tgctccgccc tcactgccgc
     3781 ctcgattgcc gcgtcgatgc acggcacccg cacgatcgga aggaacggca tcatctgctc
     3841 ctccgccacg aacgcgtgcg acgcgtcggt ctccccgaac agcagctccg tgcccgccgg
     3901 gacccgcacg ccggcggcct ccgcgaggac gctggcgtcc tggccgagga ggtcccgctt
     3961 cacgtgggcg cggccgcacc ctctgcccgg gccgtcgaag gtgaaggccg cctcggtgag
     4021 ccgcgcgatc gcgcgcgcgt cgagccggac ggcgccggcc cgctccatcg cggccatgaa
     4081 gtcgtccgcg acggattcga ggacgaacac ctccttctcg ccgatgcaga gcaggttgtt
     4141 gtcgaaggcg gcgcctgcga tgatcgacct ggcggccagg tcgagatccg ccgagtcgtc
     4201 cacgaccacc ggcgggttgc ccggcccgcc ggcgatgacg cgcttgccgc tctgcgccgc
     4261 ggccttgacg acggccgggc cgccggtcac gcagagcagc gcgacgtccg ggtggcgaaa
     4321 cacctctccg gccgcctcga tgcttgcctc ggccatcgtc gtgatgacgt tcgtcaggcc
     4381 gacttcgcgg tggatctcac ggttgaagag ggcgagcgcg tgcgccgcca cgagcgcgcc
     4441 ggcgggatgc gggctgaaca cggcggtgtt gcccgccgcc agcacgttga tggcgttgct
     4501 cgccatcgtc gggaccgagt gcgtggccgg caggatcatg ccgatcacgc cccacggcgc
     4561 ccgctcgatc acgcagaggc cggagcgatc gctccgggcg tcgctgcgca tcgcctcgac
     4621 gccgagcacg aggcgcatcg cgcggagctt ggcgatcttg tggtcgagtc ggccgatgcg
     4681 cgtctcgtcc atctccatgc ggccgagcgg ctcggcccgc tcctcgcaca ggcggcggat
     4741 gacgccgatc atccgcccgc gctcgtcgag gctcatctgc gcgacgcgcg cctgggcggc
     4801 ggcggccgcc ttcaccgcct cgtccaccgt cgcgaagacg ccgtcgccga gcggggcgcg
     4861 cttctgcttc gcggccgctg gcgtggccgg ctgcgccgcc gcccgcgcgg gggccgccgg
     4921 cgcgagcggg cgcaccgagg cgccgcctgg cgggcgagat cccggcgcgg gacgcaggac
     4981 ggccgccggc gcggcgccgg ggtacgccag cagccgggcc acgacctcct gcgcgatgtc
     5041 ggcgacgacg cgatcgcgat cggcgatcat tgcggcatct ccaccgtggc ggcgcccatc
     5101 tccaccgagt ccacgatccc gacgatgacc gcgtcggtgg ggctgtcctt cgtcatcggc
     5161 gtcatccggg ccgaactgcc ctgcgtgaag agcacgtagg tgcccagccc cgcgccgacg
     5221 ctgtcgacgg ccaccaccgt gttgccggac tccgtgagga cgccgtcctt cgccacgcag
     5281 ggctgcacga ccaggagctt cgcgccctgg aacttcgcgt gcttctgcgt cgcgacgacg
     5341 ctgccgatga ctctcgcgag caacatggtc ccgcctccgt tccctagccc gggagcgttc
     5401 ccgccacctc gtcgtgcggg cgcgggatga cgtgcacgct caccacctcg ccgaccgagc
     5461 gcgccgccac ggcgccggcg tccaccgccg ccttgacggc cgcgacgtcg cccgctagga
     5521 agatcgtgca catcccgctg ccgaccttct tccacccctt gaacgtgaca tccgccgcct
     5581 tcagggccgc gtcggcggcc tggaccaggg gcgtgaggcc gcgtgtttca atcatgccga
     5641 ttgccgtctg tgccatgtcg tcctccagcc gaacacgccg gacccgagat tcgttcccgc
     5701 ggaccggagc ggccgcgtcg cacgggtctc ggggccgtcc ctatgggagc atccccgcga
     5761 cgaggccgtc gtgcggccgg gtgatgacgt gagccgcgac cagctcgccg accgtcttgg
     5821 cggccgccgc gccggcgtcc acggccgcgc ggacgctgcc cacgtcgccg cggacgatgg
     5881 aggtgacgta gccgccgccg atctggatcc gcgccgtgag cttcacgttc gcggccttgc
     5941 acatggcgtc gctggcctca accaggccca cgagcccctt cgtttcaatc aagccgagtg
     6001 cttccaccat ggccctgctc ccttcaccag ccgcgcctac gagaggccgg ccgcggcctt
     6061 cggcccgagc ctcccgacga gcccgtccag gtcgtcgtgc gggcgcggga tcacgaggac
     6121 gctcaccacc tcgcccaggg gcttcgccgc cgcggcgccg gcgtccaccg ccgccttgac
     6181 cgccgccact tcgccttcga cgaccgcggc caccaggccg gagcccaccg gctcccaggt
     6241 cctgaaggtc acgccggccg ccttcagcat cgcgtcgacg gcctcgaaga gcgccaccag
     6301 tccgcgtgtc tcgatcatcc cgattgcagg tttcaccgcg cgctcctgtt ccggccgggc
     6361 cggcgctgcc gcccgtccct tctgctgccc ggccggcgct gccgccccgg cccgtgtccg
     6421 tcggagcggc ctcaccggcc gccgccttcc gacttgctga gctcgaactc cgtggccagg
     6481 tgcagcgcgc acgcgttggc ctcgtcggtg tccatgtgca cgttcagccg cgaggtcggg
     6541 tcgatgcggg cgacgacgcg gctgaagacc agcggggcgg ggctctccac gcgcaggcgc
     6601 atgaggtcgc cctgcttcac gccgtagtgc tcggcctcgg cgggattcat gtgcacgtga
     6661 atcgccgcgc ggatcacgcc ctgcttcagc tgcaccatcc ccgccggccc gaccacgtag
     6721 gcgccaggcg tgccggcgat gtcgcccgac agccgcaccg gcacgtcgtc gaagccgagg
     6781 aggattgcgt cggtgaaggc cagctcgatc tgcgactcct tccggagcgg cccgaggatg
     6841 cgcaggttcg ggatcgagcg gccgcggcgc ccgacgagcg tgaccgtctc cttcgcggcg
     6901 tagttgcctt cctggtagag cggccggtcc ggcgtgagct cgtggccggg cccgtacagc
     6961 acgtcgaggt ccgcgcggct cacgtgcatg tgccgggcgg agacgtgcac gacgagcggg
     7021 ttcgtgccgg ccgcaggcgc ggcgccgaac cgcgccgaga ccacctggcg cacgacctct
     7081 tcgatggccg cgcgggtgag cggcgggcgg tcgatcgtcg cggtgctagc ggccatgtgc
     7141 gcctccggcg gccccgccgc ctttgaccac ctcggccgtc gcgcgcgcca cgacccgctc
     7201 ctcgtcggcc gggacgatga ggatcggcac gcgcgaggcg gcggcggaga tcctggcggc
     7261 gccgcgcgcg gccgcgttga ggccccggtc gaggacgatg ccgaactcgc ccaggcccgt
     7321 gcagacgcct gcgcggatgt ccgggctgtt ctcgccaatc ccgccggaga acgtgaggac
     7381 gtcaactccg ccgagcgtga cgaggaacgc gcccaggtag tggcggatcg agcgaacgaa
     7441 ggcgtcgagg gcgaggcgcg cccgcgcgtt gcccgacgcg gccgcttcgt cgaggtcgcg
     7501 gatgtcgcca gacaggccgc tgatgcccgc gaggccggac tgcgacgcca gcacgcgcgc
     7561 catctcgtcg gggccgaggc cgcgcttctt catgacgaag agcgccgcga agacgtcgag
     7621 gtcgccgcag cggttgctct gcggcaggcc cgactgcggc gacatgccga agctcgtgtc
     7681 ggcggcgacg ccgtcgcgga cggcggccag gctcgagctg ccgccgagat ggcaggagat
     7741 gtgccggagg ccggcgccga ggtgcgcctg cacgtgctcg tttgccgcgc ggtggctcgc
     7801 gccgtggaag ccgtaccggc gcacacccag ctcctcgcgc cagtcgaacg gcaccgcgta
     7861 ggtcgtcgcc gccccgtcga ggcggtcgaa gaaggccgtc tcgaagaggg cgacgagggg
     7921 aacgcccggc acgcccctgg cgaacgagcg catcgcggca acgtagggcg ggttgtgggc
     7981 gggcgcgaag aaggcgaact cctccatcgc ggcgagcacg tcctcgtcca ccacgcgcgc
     8041 gccgctgacc gggccggcgt gcacggcctt gaagccgatg gcggccagct cgtccaggcg
     8101 ggcgagcggc ttgccgtcgc cggcgatgtc agcgacgcat cggcggatag ccgtttcgta
     8161 gtccgggcag tcgccgccgg tctggccgat gcgctcgatg cgtccctgcg cgaggacggt
     8221 gtcgcgctcg acggcgtaga ggcggtattt gaacgacgtg ctgccgatgt tggcgaccag
     8281 gatgttcatg gcctgaccga ttatgactgt ttttgacgat agcaaggaag gccgaggtcg
     8341 gtcgcaataa cgccgctcga cattctttgt ttcctccgcc actccctgat atcggcgcga
     8401 ctatacgatt tctgttgacg tattgcaagc aaaatatcta tattaacgac cgagaataga
     8461 ctgaaatatg actgtttttg accgataaga gacaggtatc aacccatgaa tcggtcaccc
     8521 aagccgtccg caccgcccac gctcgaccgc atcgctgagc tcggcgtcgt cggggccggc
     8581 ggcggcggct tcccggcggc cgtcaagctg gcggcgcgcg tctcgaccgt gatcgtgaac
     8641 ggcgccgagt gcgagccgct cctgcacaag gacaaggaac tgctccagca ccttgcggag
     8701 cccatgctgc gcggcctcgc ggcggcaatg gcgctgacag gcgctcgcga gggcatcatc
     8761 ggcatcaaag agaagtacac ggccgtcatg gccggcctgg agccgctgct gccgccgaac
     8821 gtacgcatcc tgccgctcag cgacacctac ccggccggcg acgaattcct gctggtgcac
     8881 gacgcggtgg gccgcatcat cccgccgggc ggcctgccga aggacgtgga cgcggtcgtc
     8941 gccaacgtcg agacgctcgc caacatcggc ctcgatcggc ccgtcacgca caagtacctg
     9001 accgtggccg gggccgtggc cgcgccggtc acgctcgcgg cgccaatcgg cacgagcatc
     9061 ggcgagctca tcgcgcgcgc cggcggcgcc gccgccgatc gcatcgccgt gctcctcggc
     9121 ggcgccatga tgggccggct cgccgcgagc ctcgacgagc cggtcacgaa gacgctcggc
     9181 ggcctcatcg tcctgcccga gtcgcacccg ctcatcgagc gctacaaggc gacgccccgg
     9241 cagatcgcgc gcatcggtcg gtccgcgtgc gaccagtgcc ggttctgcac ggacctctgc
     9301 ccgcgcttcc tcatcgggca tcccatccag ccgcacctcg cgatgcagtc gctggggttc
     9361 tcgacgagcc cgacgtcgat ggcggcgggg acgctgtact gctgcgagtg caacctctgc
     9421 agcctgtact cgtgccccga gaacctcgac ccgaagaacg tgtgcgtggc gtcgaaaccc
     9481 gcggcgcgcg agcggaagct cgagtggaag ggcacgccgg actcgatcgc cgcgcacccg
     9541 ctggccgggg accgccgcgt gccgacgaag cgcctcatcg cgaagctggg cctcgccggg
     9601 ttccgcaacg tcggcccgct cgaggatctg ggcgccgcgc cccggcgtgt cgtgctgccg
     9661 ctgaagcagc atgcgggcgc tccggccgcg ccggtcgtcc ggcccggcga ccgcgtcgct
     9721 gctggcgacc tggtggcggc cccggctgcc ggcgccctcg gcgcacgcat ccacgccagc
     9781 atcagcggcg tggtccgctc ggtggatggc gccgtcgtga tcgaggcgtg acaggtgaag
     9841 gggagtcagc cgtgccgaag ctgaagtcga tcgggctcgt cgaggtctcg tcgatcgccg
     9901 ccggctacgt ggcgctggac gccatgctca aggcggcgca ggtcgatctc ctgctcgcgc
     9961 gcacgatctg ctcgggcaag tacctcatcg tcgtgggcgg ggacgtggcg tcggtccagg
    10021 cgtcggtgga ggcgggcgcc gcgaagtgcg acgggtcgct catcgagcgg cgcgtcgtca
    10081 cgaaggtgca tcccgccgtg tttcccgcga tcggcctcgc cgtggacgtc acgccggagt
    10141 acacgaaggc gatcggcctc atcgagacgt tctcggcgtc gagcatcgtc gacgtcgccg
    10201 acgcggcgg
//
LOCUS       DAOSIF010000050        10180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6413_length_10180_cov_4.951407, whole genome shotgun sequence.
ACCESSION   DAOSIF010000050 DAOSIF010000000
VERSION     DAOSIF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6413_length_10180_cov_4.951407"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLT35_03105"
     CDS             <1..1209
                     /locus_tag="PLT35_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28242.1"
                     /translation="FPACLDLLRRRASEPAPGRVQLLVGPRQVGKTTLLLRLADALGP
                     AAVYAAADGPEASLPGFWDRLWARAGETARREGRAVLLLDEVHLLPDWTRRLKGEWDR
                     VRRRRLPVNVIATGSSSLRLATGSRESLAGRFERVMLGHWSASALARGFNLSDAGAVD
                     TTVRWGTYPGAMPLRDDPARRLAYLRDAIIEPAIGRDLTALGPVRKPGLLRQVLGVAV
                     SSPAQVVSLAKIQGQLQDSGALETIAHYLSLLEEAFLIAVLQKASARPLRQRAAPPKL
                     VVLSNGLLAAFDERGAPERASDPARFGAWVENACLAHAVNSGQQVRYWREEPLEVDAV
                     LDGSWGRWAIEVEAGDIESARLRGLSEFTRRYPDYAPLVLCEPAAVPAVERLGLRGQP
                     WPDFVMRGPC"
     gene            1230..1895
                     /locus_tag="PLT35_03110"
     CDS             1230..1895
                     /locus_tag="PLT35_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOG28243.1"
                     /translation="MRITAILFDFGGVLTPSPFGALRAWHAARGIDPELGLQTLFGPY
                     DRDTDHPWHQLERGEIPASSAIARIRQAAAALGFDLDLPSLFRAMGGSAAIRQEVVSK
                     VLALRAAGYRTALVTNNIKEYSSTWRAQIAVDDMFDAVVDSSAVGVRKPDARIYRLAL
                     EQLGAQPEESVFLDDAPGNVEGARAVGMRAILVEDDYARALAELDALLAPEAEAADGN
                     TGP"
     gene            complement(2105..2971)
                     /locus_tag="PLT35_03115"
     CDS             complement(2105..2971)
                     /locus_tag="PLT35_03115"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG28244.1"
                     /translation="MIVVTGATGKLGTLVLEGLLGKVPASELAAVVRSPAKAAGLAAR
                     GVQIRQADYGDPEALASAFRPGEKVLLISSNEIGQRAVQHAAVLDAARRAGVGLLVYT
                     SLLRADTSPMLLAGEHKTTEEAISASGLPYIILRNGWYIENYTDQLPAILARGTIAGA
                     AGRARYAAATRRDYADAAVAVLTSAWSTNATYELAGDRAFTLEELAAEVSRQSGKTVN
                     YVDLPPDEYKKVLLGAGLPEPIAVFFVDFDIAAAKGALDIDRGDLRQLIKRPTTPLSE
                     AVAEALGRLQQG"
     gene            3163..3306
                     /locus_tag="PLT35_03120"
     CDS             3163..3306
                     /locus_tag="PLT35_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28245.1"
                     /translation="MVARTLVSSARGSEGVELYGVNWEREARLSAAVRDVRAGALRAV
                     GTR"
     gene            3408..4187
                     /locus_tag="PLT35_03125"
     CDS             3408..4187
                     /locus_tag="PLT35_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28246.1"
                     /translation="MDRAGWQPEGVLASERSLAGGSSGASLGSPISRAERTSPLSRRL
                     TDFDRVLVDRGGFLLQHTLDLLAVKVVLPKGEFVVGREVLSWGTGRFWNPTDLLSPFA
                     PTDIDREVRHGVDAVRYSRPLGASGFVDLMSLPQKKGWAQGGVARAQANTGGFDVSAS
                     AAKYVSDIVFGADTAGDIGPVAVHGEVAYTLGLANLGGPGDVEIEKRFVRAGCGRVPA
                     LDGGRRADRQRRLPDRRVVARPPQRVRSSPWALFAQFGLHF"
     gene            complement(4287..6563)
                     /locus_tag="PLT35_03130"
     CDS             complement(4287..6563)
                     /locus_tag="PLT35_03130"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG28247.1"
                     /translation="MRHTHVAIFCFALVLSQRPVPAAPAPADPVPAYQRFLSPASPLD
                     MASAEKADRLAWIAFEEGKRNAYTAAPPSFAAVRLTSFLHDDGVDMSALRISADGSIV
                     TFIRGAAPNRDGWVANPSADPDGPDRAVWAVRTAGGPAWRVAGLPGGGYELAPDGRSV
                     VFVKDGQIYRALVTPARPAAAIDRAEQPFIRGWGVQSAPAWSPDGRKLAFVSTRTDHS
                     FVAVYDLARRRVTYMAPSVDFDSNPMWAADSRSLVFVRRPGLPFGRQAQQGGRGVGAP
                     VGPAAQVARGDAPAVHPDAAKVPGLTRATFKGGYAFSIWKADAETGEAAELWHDRPGD
                     ADFATIGNMMLAGHRLVFRFNVGGRGAPMEPQPAPADEWDRYYSLDLTSPDSVPVLLT
                     TTDGLIEDRTSVALSRDGTILFYCTNAKDIERRHIWAVPVGGGTPVQVTTGAGIETRP
                     TPLASGRLLATFSADWKMPQSLGLWTLPSGEAPSAAASPQRIVFPTARTGFPADAHVE
                     PQLVLTKAADGLEIHNQLFLPKDVRGGERRPAIVFVHGGPMRQMLLGYHYMQFYHWAY
                     AINQWLASEGYVVLSVNYRLGVGYGRSFRAAQNSGASGNSEYQDVVAGARYLQTRADV
                     DPERIGIWGLSYGGLLTAHALARNSDIFKAGVDLAGVHLWGTSLDPAAVSYASSPISA
                     IGGWTSPVLLVHGDDDRNVAFQQTTGLVQLLRQRDVHVELIVFPDDTHESMLHSRWMH
                     TLARMDGFFRRFLDPSRR"
     gene            6708..7808
                     /locus_tag="PLT35_03135"
     CDS             6708..7808
                     /locus_tag="PLT35_03135"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG28248.1"
                     /translation="MTIHDVRLIVRLVRGRTAEAAERLESDPDALDRLAAAAATEGLA
                     VVLLRALGALPAGFSLSGARLEQLESKRRLQSARSAALAASLDRLARAFIEDGLPFLL
                     LKGPYLAARFYGDADGREYVDLDLLVPRAGRARAWRLLENAGFARRSRALGGERLASF
                     FVHAYDFAGRATGIDVHWRLTRQPSVRVDEDALWAGAGSFALGGRTFGVLSDAHEVAF
                     HALSLLRDIERGRPKAKNIVDLVQIAASLDAAMDWDALLDAGRSDGTFGPMVNVLALC
                     LDAAGAHGLAPRLAQALSRCAGRRGPARALDRPGHFAPAWMAAGNRWWAARAYDTSVP
                     AWLLWWAVSLPFRAAARRPLAPSAGPPAEPPA"
     gene            complement(8115..9296)
                     /locus_tag="PLT35_03140"
     CDS             complement(8115..9296)
                     /locus_tag="PLT35_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28249.1"
                     /translation="MKTGYAFGRASLDADIDDPGAAAWLSEFLCPWAEPIQAGRGDVL
                     VRFDASSGAWDALARRRPAPAPSAFPCFALDSRVVCLPGWPEAGSTVVVDEEFDCFYR
                     VANRAVHVVARPGCRRARLGLMRTVREILVSRALDGGTLLDLHAAAFAAGGRAVLFAG
                     AKNAGKTTLLIHALASGRADFVANDRVFVSVDGNAATAIGVPTIVSVRPWTAAAFPAL
                     QGDGLSHAPTLSVGEDPPEGSGGAASAPVGLPNGAAWSPAQFTHRLRVARARGGTVHA
                     VVLPVIDPEVSTWALEPLAPAKAEALLGACLYGASSHPRPRTIFEAAGAAGLDAERRA
                     LMVRTLAAGAPCVSLRLGPDAYRESAAGWLRALGLAAGPGIADRPQDLARERGRTRPP
                     G"
     gene            9710..>10180
                     /locus_tag="PLT35_03145"
     CDS             9710..>10180
                     /locus_tag="PLT35_03145"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOG28250.1"
                     /translation="MTMRTFLALCFALVLAVTLGACGGSAPSQEFGKEDVQQIHALVQ
                     NFIAAYNAKDVEKIVTFFSPTATIMPANRSILGGTESVKGFFNERLNVEGAGDLEIET
                     LAVEGHGPLAYFAGAHVLYLKPAGAEPQRDRGKVMWILKKLGGQWKFDRQMMSSD"
BASE COUNT         1392 a         3642 c         3731 g         1415 t
ORIGIN      
        1 ttccccgcgt gcctggacct gctccggcgc cgcgcgtcgg aacccgcgcc cggccgcgtc
       61 cagctgctcg tcgggccgcg ccaggtgggc aagaccaccc tcctgctgcg cctggccgat
      121 gcgctcggcc ccgccgccgt ctatgccgcc gccgacgggc ccgaagcctc cctgccgggc
      181 ttctgggacc ggctgtgggc ccgggccggc gagaccgccc gccgcgaggg ccgcgccgtg
      241 ctgctcctcg acgaggtcca cttgctcccg gactggaccc ggcgcctgaa aggcgagtgg
      301 gaccgcgtcc gccgcaggcg cctgcccgtc aatgtcatcg ccacgggatc gtcctcgctg
      361 cgcctcgcca cagggtctcg cgagagcctg gcgggacgct tcgagcgcgt gatgctgggc
      421 cactggagcg catccgcctt ggcccgcggg ttcaacctgt cagacgccgg cgcggtcgac
      481 acgacggtcc gctggggcac ctacccgggc gcgatgccgc tccgggacga tccggcgcgg
      541 cgcctcgcgt acctccggga cgccatcatc gagcccgcca tcggacgcga cctgacggcg
      601 ctcgggccgg tccggaagcc gggcctgctg cggcaggtgc tcggcgtcgc ggtgagcagc
      661 ccggcccagg tcgtctcgct ggccaagatc cagggacagc tgcaggactc cggggcgctc
      721 gagacgatcg cccactacct gtccctgctg gaagaggcgt ttctcatcgc ggtcttgcag
      781 aaggcgtcgg cccggccgct caggcagcgg gctgcgccgc caaagctcgt ggtcctctcc
      841 aacggcctgt tggctgcgtt cgacgagcgg ggagcgcccg aacgcgcgtc agacccggcg
      901 cgcttcggcg cctgggtgga gaacgcctgc ctggcgcacg cggtgaacag cgggcagcag
      961 gtgcgctact ggcgcgagga gcccctcgag gtcgatgccg tactggacgg aagctggggg
     1021 cggtgggcga tcgaagtcga ggccggcgac atcgagtcgg ctcgcttgcg cggtctgtcg
     1081 gaattcacca gacgctaccc ggactacgcg ccgctcgtcc tgtgcgaacc ggccgcggtg
     1141 cccgccgtgg aacgcctggg gctcagggga caaccctggc cggactttgt catgcgcggc
     1201 ccatgctagc ggcggtgccg ggaggacgta tgcgcatcac ggccattctg ttcgacttcg
     1261 gcggcgtgct caccccgtcg cccttcggcg cgctgcgcgc ctggcacgcg gccagaggga
     1321 tcgacccgga gctgggcctc cagacgctct tcggcccgta cgatcgggac acggatcatc
     1381 cgtggcacca gctcgagcgc ggcgagatcc cggcttcctc ggccatcgcc cgcatccggc
     1441 aggccgccgc cgcgctaggg ttcgatctgg accttcccag cttattccgc gccatgggcg
     1501 gcagcgccgc gatccgccag gaggtcgtct cgaaggtcct ggcgctgcgg gcggccggct
     1561 accggacggc gctcgtcacg aacaacatca aggagtactc cagcacgtgg cgggcgcaga
     1621 tcgccgttga cgacatgttc gacgccgtcg tcgactcgtc ggcggtgggc gtgcggaagc
     1681 ccgatgcgcg catctaccgc ctggcgctgg agcagcttgg cgcgcagccg gaggagtcgg
     1741 tcttcctcga cgatgccccg gggaacgtcg aaggggcgcg ggccgtcggg atgcgcgcca
     1801 tcctggtcga ggacgattac gcgcgcgcgc tggccgaact cgacgcgctc ctggcgccgg
     1861 aggcggaggc ggctgacggg aacaccgggc cgtaggcgcg ggggccgaag agcggaccgc
     1921 aggccggacc gcaggccggg ccggccggcc tgcaggtgct tcccgatccg ctccactcat
     1981 gtggccgccg tgatggcctc gacgtcgtgg caaccacgcg ccgactggaa caggccgcgt
     2041 ccgtacgcgc cggtgccgac gcgccgccgg ctgcgggtcc cggccggact tcatgcgccg
     2101 ggcgtcagcc ctgctgcagc cggccgagag cctcggcgac tgcctcggag agcggcgtcg
     2161 tgggccgctt gatcagctgc cgcaggtctc cgcggtcgat gtccagggcg cccttcgccg
     2221 ccgcgatgtc gaagtccacg aagaacacgg caatcggctc gggcagcccg gcccccagga
     2281 ggaccttctt gtactcgtcc ggcggcaggt ccacgtagtt caccgtcttc ccgctctgcc
     2341 gggagacctc ggccgcgagc tcctccagcg tgaacgcgcg gtcgccggcc agctcgtacg
     2401 tcgcgttcgt gctccaggcc gacgtcagca cggccaccgc ggcatccgcg tagtcccgcc
     2461 gcgtcgcggc cgcgtagcgg gcccgcccgg cggctcccgc gatcgtcccg cgcgccagaa
     2521 ttgcgggaag ctggtccgtg tagttctcga tgtaccagcc gttgcgcaga atgatgtacg
     2581 gcaggcccga cgcgctgatg gcctcctcgg tcgtcttgtg ttcaccggcc aggagcatgg
     2641 gagacgtgtc ggcgcgcagc aggctggtgt agaccagcag ccccacgccg gcccgcctgg
     2701 ccgcgtcaag gacggcggcg tgctgcacgg cgcgctggcc gatctcgttc gacgagatca
     2761 gcagcacctt ttctccaggg cggaaggcgg acgccagggc ctcggggtcg ccgtagtccg
     2821 cctgccggat ctgcacgccg cgcgcggcca ggcccgccgc tttcgcggga ctccgcacca
     2881 cggccgccag ctccgatgcc gggacttttc ccaggagtcc ttccagcacg agcgtgccga
     2941 gtttcccggt cgcgccggta acgacgatca tgatcgcgct cctttccgag attgggaggc
     3001 cgcgctgccc agagcgcgcg cccttcgatc atcgtatgcg gaaacgcgcc cggacgtgcg
     3061 gacaacggcg gctgccgcca cggcgccgcc gaggccatcg agtcgccggc ggcgatggtc
     3121 acggcgctgc acctgccgcc ccgcagcgaa gttggctggc gcgtggtcgc gcgaaccctg
     3181 gtgtcgagcg cgcggggcag cgagggggtc gaactgtacg gcgtgaactg ggagcgcgag
     3241 gcgcggctct cagcggccgt ccgcgacgtg cgcgccggcg cgctgagggc cgtcggcacg
     3301 cgctagagcc gcgtgggacg aaaggggtcg ggacggttta tgcaaacaac gaaaattgaa
     3361 ttacggtttt cgttgtttgc ataaaccgtc ccgacccctt ctgctacatg gacagggcgg
     3421 gctggcagcc ggaaggggta ctggcgtcag agcgcagcct ggcaggcgga tcgtccggag
     3481 cgtcgctcgg ctcgccaatc tcgagggccg aacgcacctc cccgctcagc cgcaggctga
     3541 cggacttcga tcgcgtgctg gtggatcggg gcgggttcct gctccagcac accctcgacc
     3601 tgctggccgt gaaagtcgtc ctgccgaaag gagagttcgt cgtcggccgc gaggtgctct
     3661 cgtggggcac gggccggttc tggaacccga ccgacctgct gagcccgttc gcgccgaccg
     3721 acatcgaccg cgaggtccgc cacggcgtcg acgccgtgcg ctactcgcga ccgctcggcg
     3781 cgagcgggtt cgtcgatctc atgtccctgc ctcagaagaa gggctgggcc cagggaggcg
     3841 tcgcgcgcgc acaggcgaac acgggaggct tcgacgtgtc cgcgtccgcc gcgaagtacg
     3901 tctcggacat cgtcttcggc gcggacaccg ccggcgacat cgggcccgtg gcggtgcatg
     3961 gcgaggtggc gtacacgctc ggcctcgcca atctcggcgg gccgggcgat gtcgagatcg
     4021 agaagcggtt cgtgcgggcc ggatgcgggc gcgttccggc gcttgacggc ggccgacgtg
     4081 ctgaccggca gcgccgcctt ccggatcgcc gtgtcgtcgc tcggcctccg cagcgagtac
     4141 ggtcctcgcc ctgggccctc ttcgcgcagt tcggtctcca cttctgatcc gccgtcgagc
     4201 ttccgggccc ggccccgtca gccgaggcag cgccgcctgc cggacctgcc ggatgatggc
     4261 ggtccgagct gcgggccgcc aagacgtcag cgacgcgacg gatcgaggaa ccggcggaag
     4321 aagccgtcca tccgggcgag cgtgtgcatc caccggctgt gcagcatcga ctcgtgcgtg
     4381 tcgtcgggga acacgatcag ctcgacgtgc acgtcccgct gccgcagcag ctgcacgagg
     4441 ccggtcgttt gctggaacgc cacgtttcga tcgtcgtctc cgtggacgag cagcacgggc
     4501 gacgtccagc cgccaatcgc gctgatcggc gacgaggcgt aggacaccgc cgccggatcg
     4561 agcgaagtcc cccagagatg gacgccggcg agatcgacgc cggccttgaa gatgtccgag
     4621 ttgcgcgcga gggcgtgcgc cgtgagcagg ccgccgtagg agagccccca gatcccgatg
     4681 cgctccggat ccacgtcggc gcgcgtctga aggtacctgg cgcccgccac gacgtcctgg
     4741 tactcgctgt tgccggacgc gccgctgttc tgggccgcgc ggaacgaccg cccgtagccg
     4801 acgcccaggc ggtagttgac cgagagcacc acgtagcctt cgctcgccag ccattggttg
     4861 atggcgtagg cccagtggta gaactgcatg tagtggtagc cgaggagcat ctgccgcatg
     4921 ggcccgccgt gcacgaagac gatcgccggc cggcgctcgc ctcccctgac gtccttcggc
     4981 aggaagagct ggttgtggat ctcgaggccg tcggcggctt tcgtcaggac gagttgcggc
     5041 tcgacatggg cgtccgccgg gaagcccgtc cgcgccgtcg ggaacacgat tcgctggggc
     5101 gacgccgccg ccgagggcgc ctcgccggaa gggagcgtcc acaagcccag cgactggggc
     5161 atcttccagt cggcgctgaa cgtcgccagc agccggcccg aggccagcgg cgtcgggcgc
     5221 gtctcgatcc cggcgcccgt tgtgacctgc acgggcgtcc cgccgccgac cggcacggcc
     5281 cagatgtgcc gccgctcgat gtccttcgcg ttggtgcagt agaacaggat cgtcccgtcg
     5341 cgcgacagcg ccaccgaggt ccggtcctcg atgaggccgt cggtcgtcgt caacaggacg
     5401 ggaaccgaat cgggcgatgt gagatcgagc gagtagtagc ggtcccactc gtcggctggc
     5461 gccggctggg gttccatagg ggccccgcgc ccgccgacgt tgaagcggaa gacgagccga
     5521 tggcccgcca gcatcatgtt cccgattgtg gcgaagtcgg cgtctccggg ccggtcgtgc
     5581 cagagttccg cggcttcgcc ggtttcggca tcggccttcc agatggagaa ggcatagccg
     5641 cccttgaagg tcgcgcgcgt gaggcccggc actttcgcgg cgtcgggatg cacggcgggc
     5701 gcgtccccgc gcgcgacctg cgcggcgggc ccgaccggcg cgccgacgcc ccgcccgccc
     5761 tgctgggcct gccgcccgaa gggcaacccg ggcctgcgga cgaacacgag gctcctgctg
     5821 tcggccgccc acatcgggtt cgaatcgaag tccacgctcg gggccatgta ggtcacccgg
     5881 cgccgcgcga ggtcgtaaac ggcgacaaaa ctgtggtcgg tgcgggtgga cacgaacgca
     5941 agcttccggc cgtcgggcga ccacgccggc gcgctttgca cgccccagcc ccggatgaac
     6001 ggctgctcgg cccgatcgat ggccgcggcc ggccgcgccg gcgtcaccag cgcccggtag
     6061 atctggccgt ccttgacgaa caccacggag cggccgtctg gcgcgagctc gtagcccccg
     6121 ccgggcaggc cggccacgcg ccacgccggg cctcctgccg tgcgcacggc ccagaccgcc
     6181 cggtccggcc cgtccgggtc cgccgacgga ttggccaccc aaccgtcgcg attcggggcc
     6241 gctcccctga tgaacgtgac aatcgagccg tcggccgaga tccggagggc ggacatgtcg
     6301 acgccatcgt cgtgcaggaa gctcgtcagg cggaccgcgg cgaaggaggg gggcgccgcg
     6361 gtgtacgcgt tgcgcttgcc ctcctcgaac gcaatccagg ccagccgatc cgccttctcg
     6421 gccgacgcca tgtccagcgg agacgccggg ctcaggaacc gctggtacgc cgggaccggg
     6481 tcggcaggag ccggagcggc agggacgggg cgctgcgaca ggaccagggc gaagcagaag
     6541 atcgcgacat gggtgtgacg catgggaggc attctctcac gggccaaggc ccgggatccc
     6601 ggcgcttctt cgggttgtcg cgccggctcc gggccgggag ggagcgcgca cggcggcggc
     6661 cgtcggacgg actatgatgt ccgcctggaa gagcgcggtt gcggaagatg acgatccatg
     6721 acgtgcgcct gatcgtgcgc ctggtgcggg gacggacggc ggaagccgcc gagcggctgg
     6781 agtccgaccc ggatgctctc gaccggctcg ccgcagcggc cgcgaccgag gggcttgccg
     6841 ttgtgctgct gcgcgcgctg ggcgcgctgc cggcgggctt cagcctgtcg ggcgcccggc
     6901 tggagcagct cgagtcgaaa cggcgcctcc aatcggcgcg gtcggctgcg ctcgcggcgt
     6961 cgctggatcg cctcgcccgg gccttcatcg aggacggcct tccgtttctc ctgctcaagg
     7021 gcccctatct cgcggcccgt ttttacggcg acgcggacgg acgggagtac gtcgacctcg
     7081 acctgctcgt gccgcgcgcc ggccgcgccc gggcctggag gctcctcgag aacgccggct
     7141 tcgctcgccg atcgcgggcc ctcggcggcg agcgcctcgc gagcttcttc gtgcacgcct
     7201 acgacttcgc cggccgggcg accggcatcg acgtccactg gcgcctgacg cgccagccct
     7261 cggtgcgcgt cgacgaggac gccctgtggg ccggcgccgg ctccttcgcg ctcggcggcc
     7321 ggactttcgg cgtgctgtcg gacgcgcacg aagtggcgtt tcacgccctc tcgctcctcc
     7381 gcgacatcga gcgcggccgg ccgaaagcca agaacatcgt cgatctggtc cagatcgcgg
     7441 cgtcgctcga tgccgcgatg gattgggacg cgctgctgga cgccggtcgc agcgacggca
     7501 cgttcggccc gatggtgaac gtgctggcgc tctgcctgga cgcggcgggc gcgcacggcc
     7561 tggcgccccg gctcgcccag gccctctcgc gctgcgcggg ccggcgcggg ccggcgcggg
     7621 cgcttgatcg ccccggtcat ttcgccccgg cgtggatggc cgcggggaac cgatggtggg
     7681 ccgcgcgcgc gtacgacacg tcggtgccgg cgtggctcct ctggtgggcc gtgtcgctgc
     7741 cgttccgcgc ggcggcccgc cggccgctgg cgccgtcagc gggcccgcca gccgagcctc
     7801 cggcctgaag cgccggcgag ggccgcgcca gggagccggc tcctcccgcc gcacagccac
     7861 caggatcact gacgggttcc aggacccggc gggcgccgcc cggccggcag cggatcctcc
     7921 tcttctgcgc ggcccctggc gagcctcgcg agcatccggc cgagggcccg attggcgagc
     7981 tcgacagcgg accctgcgcc gtgtccgcgg taagcgggcc gcgacatgcg tcggtgagcg
     8041 tgacggcgag gcccgcgtcg cggcgccccg cggccagagc cggcgtgtgg tgccggccct
     8101 gacgttcggc tgcgtcagcc tggcggccgg gtgcggcccc tctctctggc cagatcctgc
     8161 gggcgatcgg cgatcccggg gcctgccgcg aggccgaggg cgcgcagcca gccggccgcg
     8221 ctctcgcgat aagcgtccgg cccaagacgg agggagacgc agggcgcccc ggccgccagg
     8281 gtgcgaacca tgagcgcgcg ccgctccgca tcaaggccgg ccgcgcctgc ggcctcgaag
     8341 atcgtccgcg ggcgcgggtg cgaactggca ccgtagaggc acgcgcccag aagagcctcg
     8401 gccttggcgg gcgcgagggg ctccagtgcc caggtgctga cttccggatc gatcacggga
     8461 aggaccacgg cgtgcacggt gccgccgcgg gcgcgcgcca cgcgcagccg atgcgtgaac
     8521 tgcgcgggcg accaggcggc gccgttcgga aggccaaccg gcgcggacgc cgcgccgccg
     8581 ctcccttcgg gcgggtcttc gccgacgctg agggttggcg cgtgcgagag gccgtcgccc
     8641 tgcagagcgg ggaacgcggc ggcggtccac ggccgaaccg agacgatcgt gggaacgcca
     8701 atggcggtgg cggcgttgcc gtccacgctc acgaacacgc gatcgttggc gacgaagtcc
     8761 gcgcgtcccg acgcgagcgc atggatcagc agcgtcgtct tgccggcgtt cttcgcgccg
     8821 gcaaacagca ctgcgcgccc cccggcggcg aacgcggcgg cgtggagatc gagcagcgtc
     8881 ccgccgtcga gcgcgcgcga gacgaggatt tcgcgcaccg tccgcatgag ccccaggcgc
     8941 gcgcgccggc acccgggcct ggctacgacg tggacggcgc ggttggcaac ccggtagaag
     9001 cagtcgaact cctcgtcgac gacgacggtg ctcccggctt cgggccagcc cggcaggcag
     9061 acaacccggc tgtcgagcgc aaagcacggg aacgccgagg gcgcaggagc cggccggcgt
     9121 cgcgcgagcg cgtcccaggc gccggaggac gcgtcgaagc gcacgagcac gtcgccgcgc
     9181 ccggcttgga tcggctccgc ccaagggcac aggaactccg acagccacgc cgcggcgccg
     9241 ggatcgtcga tatctgcgtc cagcgacgcc cggccgaaag cgtagcccgt cttcatgcga
     9301 ggacctcgat gggaatcgaa agccgcgcac cggcggtcag cgcgtcagcg ggtcgcagcc
     9361 ccgcgcgcgc gaggagcgcc tcggactcgc ccggggcgcg cccgcgcccg ccgcgtgcgg
     9421 ccgatgcgtg cagggcccgc gaggcagtgg gtccgccgac cggcttccgc cgatcagccg
     9481 cggccggatg aggatccatc ctgccatcat ctcccaactc ggccctcggg gggccaggac
     9541 gtggagcgga ggggccgcat gccagtccgc tgcccgtgtc cgtgcctggc gctggcaacc
     9601 gcaccggcgg cttccgaggc cggggcgagc gcgacgggcg agccgggcgg gcgaagcagg
     9661 cgcgtgacac ggcccggttc agaggctaga atgcccccgt tggaggccga tgaccatgcg
     9721 cacattcctg gccctgtgtt tcgcccttgt cctggccgtc accctgggcg cgtgcggcgg
     9781 ctccgcaccg tcgcaggagt tcggcaagga agacgtccag cagatccacg cgctcgtgca
     9841 gaacttcatc gcggcgtaca acgccaagga cgtcgagaag atcgtgacgt tcttctcgcc
     9901 gaccgcgacg atcatgcccg ccaaccggag catcctcggc ggcaccgaga gcgtgaaggg
     9961 cttcttcaac gagcggctca acgtcgaagg cgctggggat ctcgagatcg agacgctggc
    10021 cgtcgaaggc cacggaccgc tggcgtactt cgccggggcg cacgtgctgt acctcaagcc
    10081 cgccggcgcc gagccgcagc gcgaccgcgg caaggtgatg tggatcctga agaagctcgg
    10141 cgggcagtgg aagttcgatc gccagatgat gagcagcgat
//
LOCUS       DAOSIF010000051        10170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6419_length_10170_cov_4.095601, whole genome shotgun sequence.
ACCESSION   DAOSIF010000051 DAOSIF010000000
VERSION     DAOSIF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6419_length_10170_cov_4.095601"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PLT35_03150"
     CDS             <1..784
                     /locus_tag="PLT35_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG28251.1"
                     /translation="PCRPAGETLAGRCVGVVGVGHVGSLVVQKARALGMEPVLNDPPK
                     ARHSGSAIYRPLDDLLDCDIVTCHTPLTFDGPDPTVRLIGEAFLARLKPGAWLCNAGR
                     GGVVDEAALHRALDAGRLGAVVLDVWDHEPAIDSRLLRRVDIGTPHIAGYSLEGKLNG
                     TGMVYDAACRFLGVEPAWRLADAMPPRPAGLPIGGFAPESGAGFRSLDRAGIAALAAR
                     VAEAYPILRDDEALKATALMDDEGRGRAFDLRKMYPVRREFS"
     gene            complement(911..1870)
                     /locus_tag="PLT35_03155"
     CDS             complement(911..1870)
                     /locus_tag="PLT35_03155"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG28252.1"
                     /translation="MRPTLIVTSAVVAVALGLAAAPALAQGRGAQARQRDAARSAPSV
                     SAPRSDGSRQQSGRQAVARGTERRQSASVQNLPRATTAQRQAGAIYSAPRGSAGRQQA
                     GGQPASRSYQSGQATGLRTAPRAIPGASTQGYRSDAGRVYDQRRGGAYGARGYASYNR
                     IIRYDGRRYARPRTFVPYRPYYFSRSYYAFRPRLNIGFGLWLGYSVRYPWSWYYGGYA
                     PRVYGYGYGYGYVPGQVYYADRGPDYYGGLSFDIEPSDADLYIDGEYVGEVGQFSPYG
                     EPLTLVPGVHRIAIVRDGYMTLEWDVEVEAGMVLPYHGVMEPW"
     gene            2175..2639
                     /locus_tag="PLT35_03160"
     CDS             2175..2639
                     /locus_tag="PLT35_03160"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOG28253.1"
                     /translation="MKRTCALLSLASLCCLAAPALAQDADSIVGTWLNGEGTAHVEIQ
                     NEGGKYFGKIVWLKEPVYPADDPKGMAGKTKVDRENPDPARRNDPILGLRMLRDFRFD
                     SPGHWKDGRIYDPKNGKDYKCKIAMETPDVLKVRGFIGISLIGRTDTWTRVR"
     gene            complement(2884..3774)
                     /locus_tag="PLT35_03165"
     CDS             complement(2884..3774)
                     /locus_tag="PLT35_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOG28254.1"
                     /translation="MERTRREFLGDSALILAGAALAADAPAVLAAPGVSWPIGCFNRP
                     WTRWPYAETLKGVKAAGYAITGLLTRTKEEPFIGADATPEYLENLKRAIAAAGLTANL
                     GALRSQHDIPIEETIKGLKKEIDNAAFLGLKYVITFGMANPAQVDHYYASMAAAAAYG
                     ADKGVQVVMKPHGGSSGASEAIIAGIKQVNHPNFKIWYDAGNIIYYEGKDPVEELKPI
                     VQHVTGFCAKDCGEVKGDVMIQFGAGKVDFAGVFKVLKSAGFNGPIMVECCKVGDTPE
                     ATTENARANRVFLEKVLAAI"
     gene            4016..4144
                     /locus_tag="PLT35_03170"
     CDS             4016..4144
                     /locus_tag="PLT35_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28255.1"
                     /translation="MVLLIAKQPGRRTYAGLEVVVRRHPDGQHSVWRGLQRLSRTT"
     gene            4489..4911
                     /locus_tag="PLT35_03175"
     CDS             4489..4911
                     /locus_tag="PLT35_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG28256.1"
                     /translation="MDSGRGRLSIDELAGLGGVSRRTIRYYVQERLLPAPLGVGRGRH
                     YDESHLRRLLDVKAQQEAGWSLDEIRAGRRQAAGGGVEVPGPQGQVLARSTWRRLELA
                     PGVELHVAHDIRLPAPGRLNEIAEWCRAHLPRNPEGEA"
     gene            4913..7165
                     /locus_tag="PLT35_03180"
     CDS             4913..7165
                     /locus_tag="PLT35_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOG28257.1"
                     /translation="MNASGLVVRGSGQTIPLEHVHVDAHVVDVCARVTVRQSYRNREG
                     VPLEVVYVFPLDEGAAVCGFEATVDGVRYVGRSVERDEAFAQYDDALEAGHGAFLLDE
                     ERADVFTASLGNVMPGSAVELSITYVTELAAEGPAARFTLPTTVSPRYAPGPDQTGVS
                     PTPAEVLNPPTALDVPYAFTFEMDVAMGGTIRRIQSVTHPIEVEQDGARASVRLAQRS
                     APMDRDLVILIAADGLDAPHVTVERGAKGSAALVSVVPRFEAAAQPAEVVFLIDRSGS
                     MGGESIEEVRNALQLCLRSLTAGSRFNIVSFGTAHAALFDESRPYDEASLQEAAALVR
                     DMDADMGGTELLPALEWVLGRKAVAGMPRQVLLLTDGEVTNTDEVINLARRHAADARC
                     FTFGIGAGASQHLVKGIARASGGAAEFISPGERIEAKVLRQFARLFAPALLDPRVDWG
                     QPGAVAAADRVPPIFSGERASIYALLDEPRKGSVRLTGTLAGRPVAFDLPIDPAAAVE
                     GDTIATLAARARIRELEEAGEYLESRGSLQRRARAKAKSASAIAALAVKYQLASRETS
                     FVAVEHRETPLEGRAELRRVPVALTHGWGGVMRESHVSYLAARASAMRSYAYLDAGLA
                     LDALESCALRAEPARAPRPLDALVALQRADGSWGLDAAFAAAVSLAPSSAAIDELLRG
                     AVGDPETARRALATAVAIAWLERHAGAERVEWEMLAAKAAQWLSTSRTEPAAGAGWNG
                     WLDVARRLVR"
     gene            7437..7649
                     /locus_tag="PLT35_03185"
     CDS             7437..7649
                     /locus_tag="PLT35_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28258.1"
                     /translation="MFRGGLAEIAPSLVTRKVDRFVHAGPGSPATDGTHIRVTRRRQS
                     PVACGAGEAGRAFYRNGSRPRAVRSA"
     gene            complement(7607..8824)
                     /locus_tag="PLT35_03190"
     CDS             complement(7607..8824)
                     /locus_tag="PLT35_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOG28259.1"
                     /translation="MDSRRAPASRHDAGQPAGTPADAPTGTSAGPPRAARANAVLSVA
                     VLGLSLAAVAAVVPQHLAAGRDEAIRKGPGVTAVRRLSEWFQGIRGTSGDTDVYVLDS
                     GRPGATALVLGGTHGNEPAGYLTAIVLVENAAAAEGSVIVIPHANRSSMTHTDYQEAS
                     PRLLEFQGASGRRTFRFGSRATNPTDQWPDPDIYVHASSGQQLSGAETRNLNRAYPGR
                     PDGTFTERVAFAITSLVRAEHVDLVIDLHEASPEYPVVNAIVAHERAMKVASGAVLNL
                     EFDDITIGLEPSPKNLRGLSHRELGDATGALALLCETSNVSQGRLRGATGARQVIEGT
                     DDKYLRAARLGRLAVPFDERGWPLKQRVGRHAASVGAILASYTSAHPGRPVSLRGLPS
                     YADLTARGLEPFL"
     gene            complement(8837..8989)
                     /locus_tag="PLT35_03195"
     CDS             complement(8837..8989)
                     /locus_tag="PLT35_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28260.1"
                     /translation="MSAITIVYRLIAAGAVVLVVLELFSQRGWKEKLTAGLVLVPLIL
                     RALMIA"
     gene            complement(8986..>10170)
                     /locus_tag="PLT35_03200"
     CDS             complement(8986..>10170)
                     /locus_tag="PLT35_03200"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOG28261.1"
                     /translation="ALALAAGQGVPVGHLVEGMFSYLDVVLVLFAAMIFMKVIEANGL
                     LAELTRSLVVLLGRSPSVLLVALTIVIMFPGMITGSCTASVLGTGVIVAPVLMRMGLP
                     RDTTGAVVSSAAVYGMIAPPVNVPVMIIGGGIDMPYVGFGLILAVLTIVPAVLTTMWL
                     ARGKVERARLAEVVAESRAELAASGAGGWRRGLLYLPLAVVVALMAGPRTFPGWFPDP
                     RLPLTFLVGSALAAAAGRRVPVAAAARAGVREILPVAGILVGVGMLIEMLTLTGLRGA
                     IVVGALSIPSAFLFASIAVILPLFGGISVYGGASVLGVPFALALLGRNQIVVLAALSL
                     IGAMGSYLPPVAFTPVVAAGAIGGPYLRIVRRCAVPAAAAVVVGTLILIYANPVARVF
                     GL"
BASE COUNT         1462 a         3656 c         3651 g         1401 t
ORIGIN      
        1 cccttgccgc ccggcgggcg agacgctggc cggcaggtgc gtcggcgttg tcggcgtcgg
       61 ccacgtcgga tcgctggtcg tccagaaggc gcgcgcgctc gggatggagc ccgtgctgaa
      121 cgacccgccg aaggcgcgcc actccgggtc cgcgatctac cgcccgctcg acgacctgct
      181 cgactgcgac atcgtgacgt gccacacgcc gctcacgttc gacggcccgg acccgaccgt
      241 ccgtctgatt ggcgaggcct tcctcgcgcg gctgaagccg ggcgcgtggc tttgcaacgc
      301 gggccgcggc ggcgtggtgg acgaggcagc gctccaccgc gcgctcgacg ccggccggct
      361 cggtgccgtc gtgctggacg tctgggatca cgagcccgcg attgacagcc gcctcctccg
      421 gcgcgtggac atcggcacgc cccacatcgc gggctattcg ctcgagggca agctcaacgg
      481 caccggcatg gtctacgatg ccgcctgccg cttcctgggc gtcgaaccgg cctggcgcct
      541 cgccgacgcg atgccgcccc gtccggccgg cctgcccatc gggggcttcg cccctgaatc
      601 cggcgccggg ttccgctcgc tcgatcgcgc tggcatcgcg gcgctcgccg cgcgggtcgc
      661 cgaggcctat ccgattcttc gggatgatga ggcgctgaag gcgacggcgt tgatggatga
      721 cgaggggcga ggccgggcgt tcgacctgcg caagatgtac cccgtccgcc gcgagttctc
      781 ctgacgcgcg cgagcgagag gcggaggctt ccttcgccag cggggccgcg ccggcacatg
      841 cgaagggcgg cctcgcggcc gcccttcgcg ccctcgtccg ccgatgccgg ggcatgcccc
      901 cgaggcggcg ttaccagggt tccatgacgc cgtggtaggg gagcaccatg cccgcctcga
      961 cctcgacgtc ccactcgagc gtcatgtagc cgtcgcgcac gatcgcgatg cgatgcacgc
     1021 ctggcacgag cgtcagcggc tcgccgtagg gcgagaactg gccgacctcg ccgacgtact
     1081 cgccgtcgat gtaaagatca gcgtcggaag gctcgatgtc gaagctcagc ccgccgtagt
     1141 agtccggccc gcggtccgcg tagtagacct gaccgggcac gtagccgtac ccgtagccgt
     1201 agccatacac gcgcggcgcg tagccgccgt aataccagga ccaggggtag cgcaccgagt
     1261 agccgagcca gaggccgaac ccgatgttca gccggggacg gaacgcgtag tacgacctcg
     1321 aaaagtagta tggccggtag ggcacgaagg tccgcggcct ggcgtagcgg cgcccgtcgt
     1381 accggatgat ccggttgtac gaggcgtacc cgcgcgcgcc gtaggccccg ccgcggcgct
     1441 ggtcgtacac ccgcccggca tcggaacggt agccctgcgt cgaggcgccc gggatggcgc
     1501 gtggcgccgt ccgcaggccc gtcgcctgtc cgctctggta gctgcgagac gccggctgcc
     1561 cgcccgcctg ctggcgcccg gcactgcccc gcggagcgct gtagatcgct ccggcctgcc
     1621 gctgtgcggt cgtcgctcgc ggcaggttct gcacgctggc gctctgccgc cgctcggtac
     1681 cgcgcgccac cgcctgccgg ccgctctgct gccggctgcc gtcgctgcgc ggagcggaca
     1741 cgctcggcgc cgagcgcgct gcgtcgcgct gccgcgcctg ggcgccccgc ccctgcgcca
     1801 acgccggcgc agctgccagg cccagcgcga ctgcgacgac agcggatgtg acaatcaggg
     1861 tgggtctcat ggccccctcc tcgcttcggc ctcgcacgcg ccccactcgg gtggtgacga
     1921 ccttctggag gaccgtcgcc agtcactaga gcaacaggga tgccaatcac gatcgcgcgg
     1981 aaacacccgc agtcgagcga ggacggaatg cgtgcgcgac gactgtcgcc tttctgtgac
     2041 agctcgaccg tcctgccgcg aggtgcctgt ccgcttcctg actcccggcg cctggcgtcc
     2101 ggcccctggc tggcgaatgc cgggttccga ttctcgcttc ccgatctatc atccccctcg
     2161 gagggtcatc cacgatgaag cgaacctgcg ccctgctgtc cctggcgtcg ctgtgctgcc
     2221 tagcggcgcc tgcgcttgcc caggacgccg actcgatcgt cggaacgtgg ctgaacggag
     2281 agggcaccgc ccatgtcgag atccagaatg agggcggcaa gtacttcggc aagatcgtct
     2341 ggctgaaaga gccggtgtat cccgccgacg accctaaggg catggcgggg aagaccaagg
     2401 tggaccgcga gaaccccgat ccggcccgcc ggaacgatcc gatcctcggt ctgcggatgc
     2461 ttcgggactt caggttcgac agcccggggc actggaagga cggacgcatc tacgacccca
     2521 agaatgggaa ggactacaag tgcaagatcg cgatggagac gccggacgtc ctgaaggttc
     2581 gcgggttcat cgggatctcg ctcatcggcc gcaccgatac gtggacgcgg gtcaggtgag
     2641 cgggagcgag gtccgggtcg gcaccgtcgc cgggcggccg ctggatcggt gacgggtcgc
     2701 ccggatcctc tcctctgaac agccggacgg gcggcgccgg ccgcctcgtc ccgggtcccc
     2761 ggccgcgggg acgcccaggc tcgaacgccg gctggacgct ctcgtccgaa cgtcccggcc
     2821 cctcgagccc ggcgcgcgac gtccctggca gcggcgacga ggcggtcggg gccgccggcg
     2881 cggctagatc gccgccagca ccttctccag gaacacgcgg ttcgctcgcg cgttctcggt
     2941 cgtggcttcc ggcgtgtcgc ccaccttgca gcactcgacc atgatcggcc cgttgaagcc
     3001 ggcggacttc agcaccttga acacgccggc gaagtccacc ttgcccgcgc cgaactggat
     3061 catcacgtcg cccttcacct cgccgcagtc cttggcgcag aagccggtga cgtgctgcac
     3121 gatgggcttg agctcttcaa cggggtcctt gccctcgtag tagatgatgt tgccggcgtc
     3181 gtaccagatc ttgaagttcg ggtggttcac ctgtttgatg cccgcgatga tcgcctctga
     3241 cgcgccgctc gatccgccgt gcggcttcat caccacctgc acgcccttgt cggcgccgta
     3301 cgccgcggcg gccgccatcg acgcgtagta gtggtcgacc tgcgccgggt tggccatgcc
     3361 gaacgtgatg acgtatttca ggccgaggaa cgcggcgttg tcgatctcct tcttcaagcc
     3421 cttgattgtc tcttcgatcg ggatgtcgtg ctggctccgc agcgcgccga ggttggccgt
     3481 gagaccggcg gccgcgatgg cgcgcttgag gttctcgagg tactccggcg tggcgtccgc
     3541 cccgatgaac ggctcttcct tcgtacgggt cagcaggccg gtgatggcgt agccggcggc
     3601 cttcacgccc ttcagcgtct cggcgtacgg ccatcgggtc cacgggcggt tgaagcagcc
     3661 gatgggccag gatacgccgg gggccgccag cacggcgggc gcgtccgccg ccagcgcggc
     3721 cccggccagg atgagagctg agtctccgag gaattcccga cgtgtgcgtt ccatctgtcc
     3781 tccttcggcc accccggtgg cgggctccag gggcgcgccc gttgagagtc tataccggcc
     3841 gcgccggcgg ctggccgcac atgttccgct ccgtgggcga ggggtcgcat agcacgcgcg
     3901 agaagaccgc gacatcggcc tgctgcgtag ctgtcagctt cgcgcgccgc atcaaggcaa
     3961 aaggcctcta cacccggggc ttcaacgacg agactcgtcc gtcgacgtcg accgggtggt
     4021 gctgctgatc gccaagcaac ccggccgccg cacgtatgcc gggctcgagg tcgtggtgcg
     4081 ccgccatccc gacgggcagc actcggtgtg gcggggcctg caacggctca gccgtacgac
     4141 gtgaggggcc agctggcgga cgctgccccg gcggtgttcg gccgatcgac ccgagcgcgc
     4201 acacgccggt tggaccggtc gccagaaccg cgaccggccc acagcgccac cggcaacaac
     4261 agtgcggcga aaccggacaa atcacttgtc aaaccagacc ggacaattca cttgtcaaca
     4321 acagaggaag atacgcgggg aggacaccca cggcccctgg cccaggcact ccgcccgcgc
     4381 atcctgcggg cggcaagggt gcggcctcgg gcaccatcag gccggctctc cggcgcagcc
     4441 aaaataagac acttgacaat atgacagtta ctgtctatat tgtgtcccgt ggacagcggg
     4501 cgcggacgac tcagcatcga cgaactggcc ggcctcggcg gcgtctcccg ccgcaccatc
     4561 cgctactacg ttcaggaacg cctgcttccg gcgcccctgg gcgtcgggcg cggccgtcac
     4621 tacgatgaat cgcatctccg gcgcctcctc gacgtcaagg cgcagcagga agcgggctgg
     4681 tccctcgacg aaatccgggc cgggcggcgg caggcggcag gcggtggcgt cgaggtcccg
     4741 gggccccagg gccaggtcct cgcgcgcagc acctggaggc ggctcgaact ggcccccggc
     4801 gtcgaactgc acgtcgcgca cgacatcagg ctgccggccc ccggccggct gaacgagatc
     4861 gcggagtggt gccgtgcgca ccttccgcgc aacccggaag gagaggccta gcatgaacgc
     4921 gtctggactc gtcgttcgcg gcagcggtca gaccattccc ctcgagcacg tgcacgtcga
     4981 cgcccacgtc gtcgacgtct gcgcccgcgt caccgtgcgc cagtcgtacc gcaaccgcga
     5041 gggcgtgccg ctcgaggtcg tctacgtctt cccgctggac gagggcgcgg ccgtctgcgg
     5101 cttcgaggcg acggtggacg gcgtccgcta cgtcgggcga tcagtcgagc gcgacgaggc
     5161 cttcgcgcag tacgacgatg cgctggaggc ggggcacggg gcgttcctgc tggacgagga
     5221 gcgcgcggac gtgttcacgg cgagcctcgg caacgtgatg cccggctcgg ccgtcgagct
     5281 gtcgatcacg tacgtcaccg agctggcggc ggaagggccg gcggcgcgct tcacgctgcc
     5341 cacgaccgtc tcgccgcgct acgcacctgg gcccgatcag actggcgtca gcccgacgcc
     5401 ggccgaggtg ctcaacccgc cgacggccct cgacgtgccg tatgcgttca cgttcgagat
     5461 ggacgtggcg atgggcggga cgatccgcag gatccagtcg gtcacgcatc ccatcgaggt
     5521 ggagcaggac ggcgcgcgcg cgagtgtgcg cctcgcgcag cggagcgccc cgatggaccg
     5581 ggacctagtc atcctcatcg cggccgacgg gctcgacgcg ccgcatgtga cggtggagcg
     5641 cggcgcgaag gggagcgcgg cgctcgtgtc cgtcgtgccg cggttcgagg cggccgcgca
     5701 gccggccgag gtcgtgttcc tcatcgaccg gtcgggctcg atgggcggcg agtcgatcga
     5761 agaggtccgc aacgcgctgc agctgtgcct gcggtcgctc acggccgggt cccgcttcaa
     5821 catcgtctcg ttcggcaccg cgcacgcggc gctcttcgac gagagccggc cgtacgacga
     5881 agcgtccttg caggaggccg cggcgctcgt gcgggacatg gacgccgaca tgggcggcac
     5941 cgagctgctg ccggcgctgg agtgggtgct tgggcggaag gccgtggccg ggatgccgcg
     6001 ccaggtcctg ctcctgaccg acggcgaagt gaccaacacc gacgaagtga tcaatctcgc
     6061 gagaaggcac gcggccgacg cgcgctgctt cacgttcggc atcggcgcgg gggcgagcca
     6121 gcacctggtg aagggcatcg cccgggcgag cggcggcgcg gccgagttca tcagcccggg
     6181 cgagcgcatc gaggcgaaag tgctgcggca gttcgcgcgg ctcttcgcgc cggcgctgct
     6241 cgacccgcgg gtggactggg ggcagcccgg cgccgtggcg gccgcggacc gcgtgccgcc
     6301 gatcttcagc ggcgagcgcg cgtccatcta tgccctgctg gacgagccgc ggaaggggag
     6361 cgtgaggctg accggcacgc tggccggccg gcccgtcgcc ttcgacctgc cgatcgaccc
     6421 cgcggccgcg gtcgaaggcg acacgattgc gaccctggcg gcgcgggcgc gcatccgcga
     6481 gctggaggag gccggcgagt acctggagag ccgcggatcg ctgcagcggc gcgctcgggc
     6541 gaaggcgaaa tccgcgtcgg cgatcgcggc tcttgccgtg aagtaccagc tcgcctcgcg
     6601 cgaaacgtcg ttcgtggccg tcgagcaccg cgagacgccc ctcgagggac gcgccgaact
     6661 gcggcgcgtc ccggtggcgc tgacgcacgg atggggcggt gtcatgcgcg agagccacgt
     6721 ctcgtatctc gctgcccgcg catccgccat gcggtcgtac gcctatctcg atgccgggct
     6781 cgcgctggac gccctggaaa gctgcgccct cagggcggag ccggcgcggg cgccacggcc
     6841 gctcgacgcg ctcgtcgcgc tccagcgcgc ggacggatcg tggggcctcg acgccgcgtt
     6901 cgccgcggcc gtgtcgctgg cgccgtcgtc tgccgcaatc gacgagctcc tgcgcggcgc
     6961 cgtcggcgat ccggagaccg cgcggcgcgc gctcgcgaca gcggtcgcga tcgcgtggct
     7021 cgagcggcac gctggagccg agcgggtcga gtgggagatg cttgccgcga aggcggcgca
     7081 gtggctgtcg acgtcgcgca cggagccggc cgccggcgcg ggctggaacg gctggctcga
     7141 cgtcgcgcgc cggctcgtgc gctgagcgac gggcttgcgt gaggcgcgcc tcgtccgacg
     7201 cccggaggaa ccgggggtcc aagaggcgct gttcccgagc caggtccgcc gcgaggtgcc
     7261 cgctcgtccg ggccggcgcg ggcgccgtcg agcgttcact cgggacgccg acgcgcgcgg
     7321 cggcgcctgc tgcgccgtga accagcgccg cgccggcggc ccgctggcgg cgatcacgct
     7381 ggccggcagg acgcggcggc acgggcagcg acgtcgacgg ttggcggcag gccgtcgtgt
     7441 tccgcggcgg cctggccgag atcgcgccct cgctcgtcac gcggaaggtc gatcggttcg
     7501 tccacgccgg ccccggcagc ccggccaccg acggcacgca catccgggtg acgcgaaggc
     7561 ggcagtctcc cgtggcgtgc ggcgccggag aagccgggcg cgccttctac aggaacggct
     7621 ccaggccccg cgccgtgagg tcggcgtagg aaggcaggcc gcgcagcgac acgggcctcc
     7681 ccgggtgggc cgacgtgtac gacgccagga tggccccgac ggaggcggca tgcctcccca
     7741 ctcgctgctt cagcggccac ccccgctcgt cgaacggcac ggccagccgg ccgagcctcg
     7801 ccgcgcgcag gtacttgtca tcggtgccct cgatgacctg ccgcgcgccg gtcgcgcccc
     7861 gcaggcgccc ctgcgacacg tttgacgtct cgcacagcag cgccagcgcg ccggtcgcgt
     7921 cccccagctc ccggtgcgac agcccgcgca ggttcttcgg cgacggctcc aggccgatcg
     7981 tgatgtcgtc gaactcgaga ttcagcaccg caccggatgc gaccttcatc gcccgctcgt
     8041 gcgccacgat ggcgttgacc actgggtact cgggggacgc ctcgtgcagg tcgatgacga
     8101 ggtccacgtg ctccgcccgg acgagcgacg tgatggcaaa cgcgacgcgc tcggtgaacg
     8161 ttccgtcggg ccggccggga tacgcgcggt tcaggttccg cgtctccgcc cccgacagct
     8221 gctgccccga cgaggcgtga acgtagatgt ccgggtcggg ccactggtcc gtcgggttag
     8281 tcgcccggct cccgaaccgg aacgtgcgcc gcccggacgc gccctggaac tcgagcaggc
     8341 gcggcgacgc ctcctggtag tccgtgtgcg tcatcgacga tcggttcgcg tgcgggatga
     8401 cgatgaccga gccctccgcg gcggcggcgt tctccaccag gacgatggcg gtgagatagc
     8461 cagccggctc gttgccgtgc gtgccgccga ggacgagggc ggtggccccg ggcctgccgg
     8521 aatccagcac gtacacgtcc gtgtcgccgc tcgtgccgcg gatcccctgg aaccactccg
     8581 acagccggcg cacggcggtg acgcccggcc ccttcctgat cgcctcgtcg cgccccgccg
     8641 cgaggtgctg cggcacgact gcggcgacgg ccgcgaggct gagccccagc acggcgacgg
     8701 acaggacggc gttcgcacgg gcggcgcgcg gcgggccggc tgacgtgccg gtcggcgcgt
     8761 cagcgggcgt gccggcaggc tggccggcgt cgtggcgcga agcgggtgct cgtcgagagt
     8821 ccatgcggca gtcctcctac gcgatcatca gcgcgcgcag gatcagcggg acgaggacca
     8881 gcccggccgt gagcttctcc ttccaccccc gctgcgagaa cagctccagc acgaccagca
     8941 cgaccgcgcc ggcggcgatc aggcggtaga cgatggtgat ggcgctcaca ggccgaacac
     9001 cctcgcgacg gggttcgcgt agatcaggat cagcgtcccg acgacgacgg cggcggcggc
     9061 cggcacggcg cagcgccgca cgattcgcag gtacggcccg ccgatcgccc cggcggcgac
     9121 caccggggta aacgccacgg gcggcaggta gctgcccatc gcgccgatga gcgacagcgc
     9181 cgccagcacg acgatctggt tgcgcccgag cagcgcgagg gcgaacggca cgccgaggac
     9241 gctggcgccg ccatacaccg agatcccgcc gaacagcggc aggatcacgg cgatgctcgc
     9301 gaacaggaac gcgctgggga tcgacagcgc gccgaccacg atcgccccgc gcaggccggt
     9361 gagcgtcagc atctcgatca gcatgcccac gccgacgagg atgcccgcga cgggcaggat
     9421 ctcgcgcacg cccgcgcgcg cggcggccgc gacgggcacg cgccgcccgg ccgccgccgc
     9481 gagggcgctg ccgaccagga acgtcaaggg cagccgcggg tcggggaacc acccggggaa
     9541 cgtccgcgga ccggccatca gggcgacgac caccgcgagc ggcaggtaga gcagcccgcg
     9601 ccgccagccg ccagcgccgc ttgcggcgag ctcggcccgg ctctccgcca ccacctcggc
     9661 cagcctcgcc cgctccacct tccccctcgc cagccacatc gtcgtcagca cggcgggcac
     9721 gatggtgagc acggcgagga tgaggccgaa gccgacgtac ggcatgtcga tgccgccgcc
     9781 gatgatcatc accggaacgt tgaccggcgg cgcgatcatg ccgtacacgg ccgcgctcga
     9841 gacgaccgcc cccgtcgtgt cgcgcggcag ccccattcgc atcagcaccg gggcgacgat
     9901 gacgcccgtg ccgaggaccg aggccgtgca cgagccggtg atcatgccgg ggaacatgat
     9961 gacaatcgtc agcgcgacga ggaggacgga cggcgagcgt ccgagcagca cgaccaggct
    10021 gcgcgtcagt tcggccagca ggccgttcgc ctcgatgacc ttcatgaaga tcatcgcggc
    10081 gaagagcacg agcaccacgt cgaggtagct gaacatgccc tcgacgaggt ggccgacggg
    10141 gacgccctgg cccgccgcca gcgccagcgc
//
LOCUS       DAOSIF010000052         9923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6594_length_9923_cov_4.224260, whole genome shotgun sequence.
ACCESSION   DAOSIF010000052 DAOSIF010000000
VERSION     DAOSIF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6594_length_9923_cov_4.224260"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLT35_03205"
     CDS             complement(<1..631)
                     /locus_tag="PLT35_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28262.1"
                     /translation="MSPIRTVIMGAAGRDFHNFNVVYRDNPAYDVVAFTAAQIPNIDG
                     RKYPASLAGRLYPKGIPILAEADLEAVIRDRKVQEVVFSYSDVSYAHVLGLGAVVNAA
                     GAHFKLLGAGATMLKSAKPVIAVCAVRTGSGKSQTTRRIARMLREQGFTVAAVRHPMP
                     YGNLERQRVQRYAALSDLKAHRCTIEEIEEYEPHIVSGTITYAGVDYGDI"
     gene            complement(809..2008)
                     /gene="rocD"
                     /locus_tag="PLT35_03210"
     CDS             complement(809..2008)
                     /gene="rocD"
                     /locus_tag="PLT35_03210"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOG28263.1"
                     /translation="MDRQPTSRALIEMEEQYGARNYHPLDVVIERGEGVWVYDVEGRK
                     YLDCLAAYSAVNQGHCHPRIMEAAVGQLKRVTLTSRAFRNDQLPRFYRLLHELTGYDL
                     SLPMNSGAEAVETAVKAARKWGYAVKGIPADQAEIVVCANNFHGRTVTIVSFSTEPQY
                     REGFGPLTPGFTVVPFGDAAALESAVTPNTAAFLVEPIQGEAGIVIPPDGYLRQAAAI
                     CKAHNVLFVCDEIQSGLGRTGRLFAHEWEGVRPDMVVIGKALSGGYYPVSAVLGSREV
                     LGVFRPGDHGSTFGGNPLACAVARAALQVLIDEQLAARSAELGAYFLGRLQALQSAVI
                     KEVRGRGLWIGIELTCPARPYCERLMQEGMLCKETHDRVIRLAPPLTITRDEIDWALE
                     RLQKVLC"
     gene            complement(2085..3050)
                     /gene="pyrB"
                     /locus_tag="PLT35_03215"
     CDS             complement(2085..3050)
                     /gene="pyrB"
                     /locus_tag="PLT35_03215"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOG28264.1"
                     /translation="MKLEHVIESQQFTVPLLMELFARARQMERVAARGGTRDYAGRIM
                     ASLFYQPSTRTRFSFEAAMYRLGGRVLSTEHAGAFSSEVEGEQLEDTIRIISGCSDVI
                     VLRHHEEGGARRAARVSRVPVVNAGDGGGGQHPTQALLDLYTIYRERRTLDGLTIAIV
                     GELDRGRTARSLAYLLGKFERVKIHFVSPPEVRMGRDILDHLDEHDVWYDLSTDVEPI
                     AGEVDVIYQTRIRPDRVADGASLARCAIDERLLRRMKPDAMILHPLPRTVEIDKAVDG
                     DPRALYFKQAGNGLFVRMALLTLLFERDDEEPDAPWPPQRRREDR"
     gene            complement(3081..4025)
                     /gene="arcC"
                     /locus_tag="PLT35_03220"
     CDS             complement(3081..4025)
                     /gene="arcC"
                     /locus_tag="PLT35_03220"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOG28265.1"
                     /translation="MAKTALIAIGGNSLIRAGEKGAIAEQLANTRRTAAAIVHIIRAG
                     YRVVLTHGNGPQVGAALLRSERAADRVYTHPLDVCGAATQGEIGYLLQQSLQAELALA
                     GLTTPVVTVVTQVVVSPSDPAMERPTKPIGPFYSAREARAKQDQFGWAIVEDASRGYR
                     RVVPSPEPLEIVEEGAIRRLIDTGVIVIAAGGGGIPVVRRGHELRGVEAVVDKDRSSA
                     LLAARLQTRSLIISTDADRVYVNYKKPGQRPLGRVTAAEIEAHWREGQFPLGNMGPKI
                     ESALRFLRDGGREAIITSYERLPDAVRGEAGTHIVAGG"
     gene            complement(4071..4988)
                     /gene="argF"
                     /locus_tag="PLT35_03225"
     CDS             complement(4071..4988)
                     /gene="argF"
                     /locus_tag="PLT35_03225"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG28266.1"
                     /translation="MRVPDFLTIRDFTTAELQHLLDLARDIKAHPGDFGDSLKGKALA
                     LIFEKPSLRTRVSFDAGIHQLGGFSIYLSPAEINLGKRESVHDVAKNLERMVQGIMIR
                     TFAHEIVEKMAEWASIPVINGLTDSSHPCQAMADFLTILEHKGRLAGLKLAYVGDSNN
                     VSNSLMFAAARFGTHIAIASPEGYQPAPSLLEWTRAEGAKTGSVCTVTADPAEAVDGA
                     DVVYTDTWASMGQEAEAYARKAVFRPYQVNARLFARARPDALFMHCLPAHRGDEVTDE
                     VIDSPNSVVFDEAENRLHVQKAILLELMK"
     gene            complement(5357..6811)
                     /locus_tag="PLT35_03230"
     CDS             complement(5357..6811)
                     /locus_tag="PLT35_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28267.1"
                     /translation="MPPLIVPRAEHTLSRKDVDPEALKVLYRLKDAGYTAYLVGGGVR
                     DLLLGRRPKDFDVGTDAHPYQVKKLFRNCFIIGRRFRLAHVRFGAKVIEVATFRRQIT
                     DEEAAAAEAREAEARAREAGAPAAEAGAPDAAREAGARDGEGAGPEPRQEGARGGEGA
                     GPEPRQEGARGGRRARRPPPLQHENTFGTPEEDAFRRDFTINGLFYDIATFSIIDYVG
                     GLDDLRARVVRCIGDPVVRFREDPVRMQRAVSLAARLGFAIDPPVLDALAAERHRLAL
                     AAPARLVEEYYKILRSGAAEATFRDLRRLGLLEPLSEALQQRAGDALWASLARLDAHR
                     RRFAAAPAALTTGVLLGSLIVPLGFSQGGGPAWMEGGDRPGLSLGVLPLSRRDVERVR
                     QVLMLQRRLREPSRSPHARRMLMRRSAFPDALTWLEIHGDAPDALEYWRGRQGGDAQP
                     AEAGDAPRDPAGPPGAPASRRRRRRRGRRRPRPE"
     gene            complement(6979..9669)
                     /gene="polA"
                     /locus_tag="PLT35_03235"
     CDS             complement(6979..9669)
                     /gene="polA"
                     /locus_tag="PLT35_03235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOG28268.1"
                     /translation="MPTLYLIDGSAQMYRAYHAIRGLTGPDGRSTNAVFGFVSMLRKL
                     LAEHRPDAIAVAFDLEGPTFRDGLAEDYKANRAPMPADLADQIPWIHQACAALGVPVV
                     TAAGFEADDVIGTLAKQGEAAGYDVAIVTGDKDFFQLVGGRVRVFNPRDEGTWYDAAG
                     VTDKFGVPPERVIDALAIMGDASDNVKGVPGIGEKGARDLLAQFGSLDALLEGAAGVA
                     QKRYREALLAHADAARASRELVTIRTDVPLDLGLEPLRYRGPDREACYALFSQLGFRT
                     LTGDFAPAAAAAATDYAVVQSLDELAALVGSLRAGGRAGLRVIGDDAAPMRARIVGIA
                     LAGGERRARYVPLGHRALDECANLDAAAALGALAPLLEDERVEKIGHDVKYDAIVLAR
                     HGVTVAGPGFDTMLASYLLDSTRAPHGLETLSIEHLGGRPLTEEDVYGKGARAAAPEN
                     LPAAATLAYAAERADLPLRLAGPLRRSLAAEGLDELYRGLEWPLVPVLAAIERAGVRI
                     DTAALASLSASLEREMEERSRRIFELAGGPFNIGSPKQLAEILFEKLKLPVLKKTGAT
                     RAASTSVEVLEELALAHEIPRLILEWRSMQKLKGTYVDALPGLVHPETGRVHTSFNQA
                     VAATGRLSSSDPNLQNIPVRTELGRRIRGAFVADPGCVLISADYSQIELRVLAHLSGD
                     EALIEAFRRGEDIHDRTALAIFGADSGLDRKEMRSRAKMVNYALLYGKTAFSLARDIG
                     VPQQAAQAFIDAYFAGFPKVRAFIDRTLEDGRATGMVKTLAGRRRPVPGLTSANGQAR
                     SAAERAAVNMPVQGTAADILKRAMIDIHRALEARPARAGGRRARMILTVHDELLFEAP
                     RDEAAEVAALVRRHMEGAADLAVPLTVEAGIGENWKEAKR"
BASE COUNT         1297 a         3527 c         3698 g         1401 t
ORIGIN      
        1 ggatgtcgcc gtagtccacg ccggcgtagg tgatcgtgcc gctgacgatg tgcggctcgt
       61 actcctcgat ttcctcgatg gtgcaccggt gcgccttcag gtccgagagc gccgcgtacc
      121 gctgcacgcg ctgccgctcg aggttcccgt agggcatggg gtggcggacg gccgccacgg
      181 tgaagccctg ctcgcgcagc atccgcgcga tgcgcctcgt cgtctggctc ttgcccgagc
      241 cggtgcgcac ggcgcagacc gcgatcacgg gcttcgcgct cttcagcatc gtcgccccgg
      301 cgcccagcag cttgaagtgg gcgccggcgg cgttgacgac ggccccgagg ccgaggacgt
      361 gggcgtagga gacgtcgctg tacgagaaga cgacctcctg caccttccga tcgcggatga
      421 cggcctcgag gtccgcctcg gcgaggatcg ggatgccctt gggatagagc cggccggcga
      481 gcgacgcggg gtacttgcgg ccgtcgatgt tcgggatctg cgcggccgtg aaagccacga
      541 cgtcgtaggc ggggttgtcg cggtagacga cgttgaagtt gtggaagtcg cgccctgcgg
      601 cgcccatgat gacggtgcgg atggggctca tgacacctca agaaacaggg gctagggact
      661 aggcgctggg cgctagtcgg tcaacaagca acgggctagg gactaggcgc tgggcgccag
      721 tcaactacag gcgacaggcg ctggggacca ggcgttaggg gccagtcgac cagcgccgtc
      781 aaaccgctca acgtgccaaa ctccggtgct aacacagcac cttctgcaaa cgctccaggg
      841 cccagtcgat ctcgtcgcgc gtgatcgtga gcggcggcgc gaggcggatg actcggtcgt
      901 gggtctcctt gcagagcatg ccctcctgca tcaggcgctc gcagtacggg cgcgccgggc
      961 aggtgagctc gatgccgatc cacaggccgc ggccgcgcac ctctttgatc acggcgctct
     1021 gcagcgcctg cagccgcccg aggaagtacg cgccgagctc ggcggatcgc gccgcgagct
     1081 gctcgtcgat cagcacctgc agcgcggcgc gcgcgacggc gcaggcgagg gggttgccgc
     1141 cgaacgtgct gccgtggtcg cccggccgga acacgcccag cacctcgcgg ctgccgagga
     1201 cggccgacac gggatagtac ccgccggaca gcgccttgcc gatgaccacc atgtcgggcc
     1261 gcacgccctc ccactcgtgg gcgaacagcc ggcccgtgcg gccgaggccg gactggatct
     1321 cgtcgcacac gaacagcacg ttgtgcgcct tgcagatggc ggcggcctgc ctcaggtagc
     1381 cgtccggcgg gatgacgatg cccgcctcgc cctggatggg ctcgacgagg aacgccgccg
     1441 tgttcggcgt gacggccgat tcgagggccg ccgcgtcgcc gaagggcacg acggtgaagc
     1501 cgggcgtcag cggcccgaag ccctcgcggt actgcggctc ggtggagaag ctgacgatcg
     1561 tcaccgtgcg gccgtggaag ttgttcgcgc agacgacgat ctcggcctgg tccgccggga
     1621 tgcccttcac cgcgtagccc cacttgcgcg cggccttcac ggcggtctcc accgcctcgg
     1681 cgcccgagtt catcggcagc gacaggtcgt acccggtgag ctcgtgcagc agccggtaga
     1741 agcgcggcag ctggtcgttg cggaacgcgc gcgaggtcag cgtgacgcgc ttcagctgcc
     1801 cgacggccgc ctccatgatg cgcgggtggc agtggccctg gttcacggcc gagtaggccg
     1861 ccaggcagtc gaggtacttc ctgccctcca cgtcgtacac ccacaccccc tcgccgcgct
     1921 cgatgacgac gtcgagcggg tggtagttgc gggcgccgta ctgctcttcc atctcgatga
     1981 gcgcgcgcga cgtcggctgt cgatccatct gctcccacct gtccttccct tggcccgggg
     2041 ttcccgcggg cggccggcgg ccgccgccgc gccgccccgc gcggctaccg atcctcccgc
     2101 ctccgctgcg gcggccacgg cgcgtccggc tcctcgtcgt cccgctcgaa cagcagcgtc
     2161 agcagggcca tgcgcacgaa caggccgttc ccggcctgct tgaagtagag ggcccgcgga
     2221 tcgccgtcca ccgccttgtc gatctcgacc gtccgcggca gggggtggag gatcatcgcg
     2281 tccggcttca tgcgccgcag gagccgctcg tcgatcgcgc agcgggcgag ggacgcgccg
     2341 tcggcgacgc ggtcgggccg gatgcgcgtc tggtagatga cgtccacctc gcccgcgatg
     2401 ggctccacgt cggtggagag gtcgtaccag acgtcgtgct cgtcaagatg gtccaggatg
     2461 tcccggccca tccgcacctc gggcgggctc acgaagtgga tcttcacccg ctcgaacttg
     2521 cccagcaggt aggccagcga ccgcgccgtc cggccgcggt cgagctcgcc gacaatcgcg
     2581 atggtcaggc cgtcgagggt gcggcgctcg cggtagatcg tgtagaggtc gagcagcgcc
     2641 tgggtggggt gctggccgcc gccgccgtcg ccggcgttga cgacgggcac gcgcgacacg
     2701 cgcgcggcgc gccgggcccc gccctcctcg tggtggcgca ggacgatgac gtcggagcag
     2761 cccgagatga tgcggatcgt gtcttcgagc tgctcgcctt cgacctctga cgagaaggcg
     2821 ccggcgtgct cggtcgagag gacgcggccg cccagccggt acatggccgc ctcgaacgag
     2881 aagcgcgtgc gcgtggaggg ctggtagaag agcgacgcca tgatgcggcc cgcgtagtcg
     2941 cgcgtgccgc cgcgcgccgc gacgcgctcc atctggcgcg cccgggcgaa gagctccatc
     3001 aggagcggca cggtgaactg ctgcgactcg atgacgtgct cgagtttcat gacatcgatc
     3061 agaacccgga actcggcatt ctatcccccc gcgacaatgt gcgtccccgc ctccccccgc
     3121 acggcgtccg gcagccgctc gtaggacgtg atgatcgcct cgcggccgcc gtcccgcagg
     3181 aagcgcagcg ccgactcgat cttcgggccc atgttgccga gcgggaactg gccctcccgc
     3241 cagtgggcct cgatctccgc ggccgtgacc cggcccaggg ggcgctggcc gggcttcttg
     3301 tagttcacgt agacgcggtc ggcgtcggtg gagatgatga gcgagcgcgt ctgcaggcgc
     3361 gcggcgagca gcgccgacga gcggtccttg tcgacgacgg cctcgacgcc gcgcagctcg
     3421 tggccccggc gcaccacggg gatgcccccg ccgccggccg cgatgacgat cacgccggtg
     3481 tcgatgagcc gccggatggc gccctcctcg acgatctcca gcggctcggg cgacggcacg
     3541 acgcgccggt agccgcgcga ggcgtcctcg acgatggccc agccgaactg atcctgcttc
     3601 gcccgcgcct cgcgcgccga gtagaacggc ccgatgggct tggtcgggcg ctccatggcg
     3661 gggtcggacg gcgacacgac cacctgcgtc accacggtga cgacgggcgt cgtcaggccg
     3721 gccagcgcga gttcggcctg cagcgactgc tgcagcaggt agccgatctc gccctgcgtc
     3781 gcggcgccgc acacgtcgag ggggtgggtg tacacccggt cggccgcgcg ctccgagcgc
     3841 agcagcgccg cgccgacctg ggggccgttg ccgtgggtga ggaccacgcg gtacccggcg
     3901 cggatgatgt ggacgatggc cgccgccgtg cggcgcgtgt tggcgagctg ctcggcaatc
     3961 gcgcccttct cgcccgcgcg gatgagcgag ttgccgccga ttgcgatgag cgccgtcttc
     4021 gccatgagcc cccgcccgcg ccgtcctccc gcgcgccgcc gccgcgcgcc ctacttcatg
     4081 agctcgagca gtatcgcctt ctgcacgtgc agccggttct cggcctcgtc gaagaccacc
     4141 gagttcggcg agtcgatcac ctcgtcggtc acctcgtcgc cgcggtgcgc cggcaggcag
     4201 tgcatgaaaa gggcgtccgg cctggccctg gcgaagagcc gcgcgttcac ctggtagggc
     4261 cggaagaccg ccttgcgcgc gtaggcctcg gcttcctggc ccatgctcgc ccaggtgtcg
     4321 gtgtagacca cgtccgcgcc gtcgaccgcc tcggccgggt ccgccgtcac cgtgcagacg
     4381 gagcccgtct tcgccccttc ggcgcgcgtc cactcgagca gcgacggcgc cggctggtac
     4441 ccctcgggcg aggcgatggc gatgtgcgtc ccgaagcgcg cggcggcgaa catgagcgag
     4501 ttcgacacgt tgttgctgtc gccgacgtag gccagcttca ggcccgcgag gcggcccttg
     4561 tgctcgagga tggtgagaaa gtcggccatc gcctggcacg ggtggctgct gtcggtcagg
     4621 ccgttgatga cggggatgga cgcccactcg gccatcttct ccacgatctc gtgggcaaag
     4681 gtgcggatca tgatgccctg caccatccgc tcgaggttct tggcgacgtc gtgcaccgac
     4741 tcgcgcttgc cgaggttgat ctcggcgggc gagaggtaga tggagaagcc cccgagctgg
     4801 tggatgccgg cgtcgaagga cacccgcgtg cgcagcgacg gcttctcgaa gatcagcgcg
     4861 agggccttgc ccttgaggct gtcgccgaag tcgccggggt gggccttgat gtcgcgcgcg
     4921 aggtccagca ggtgctggag ctcggcggtg gtgaagtcgc ggatcgtgag gaagtcaggc
     4981 acgcgcatgg cggatctccc acggacgaag cccggagcct gtcacggcag ggcccgcggc
     5041 cggggcggcg cgccgccgtc cgccggaccc gcacccgatt cgatcgaccg cccgcaatcg
     5101 cctgcatgcc gggccgacac gtcgtcccgt ctctgaatcc tcgatgggcc gggagaggcg
     5161 agaagaaccg gggtagcccc cggggacggg attgtacccc cgcgcccatg cggaagggaa
     5221 gcggcggccg cggccgcggc gtgggcggcg aagcgcgcgg ggtcggcccg cgcgacgccg
     5281 cggaaaggcg gaagcgcggg tggggcgagc ggagtcgcgg ggtgccgcgc ccagccgaac
     5341 ggcagggccg gcgcgactat tcgggccgcg gacgccggcg gccgcggcgg cggcgccggc
     5401 ggcggctggc gggggcgccg ggtgggcccg cgggatcgcg cggcgcgtca cccgcctccg
     5461 ccggctgcgc gtcgccgccc tgccggcccc gccagtactc gagcgcgtcg ggcgcgtcgc
     5521 cgtggatctc gagccaggtc agcgcgtcgg ggaacgccga gcggcgcatc agcatgcgcc
     5581 gggcgtgggg cgagcggctg ggctcgcgca gccggcgctg cagcatcagc acctggcgga
     5641 cgcgctcgac gtcgcggcgc gacaggggca ggacgccgag gctcaggccc ggccgatccc
     5701 cgccctccat ccacgcgggc ccgccgccct gcgaaaagcc gaggggcacg atgaggctgc
     5761 cgagcagcac gccggtcgtc agcgcggccg gggcggcggc gaagcgccgc cggtgcgcgt
     5821 caagacgggc gagcgacgcc cagagcgcgt ctccggcccg ctgctgcagc gcctcgctca
     5881 agggctcgag caggcccagg cgccgcaggt cgcggaaggt ggcctcggcc gcgccggaac
     5941 ggaggatctt gtagtactcc tcgacgaggc gcgcgggagc ggccagcgcg agccggtgcc
     6001 gctcggcggc gagggcgtcg aggacgggcg ggtcgatcgc gaagccgagc cgcgcggcga
     6061 gcgacacggc gcgctgcatg cgcacggggt cctcgcggaa ccgcacgacg ggatcgccga
     6121 tgcagcgcac gacgcgcgcg cgcaggtcgt cgaggccgcc gacgtagtcg atgatcgaga
     6181 aggtcgcgat gtcgtagaag aggccgttga tggtgaagtc gcgccggaag gcatcctctt
     6241 ccggcgtgcc gaaggtgttc tcgtgctgca gcggaggcgg ccgccgcgcg cggcggccgc
     6301 cgcgcgcccc ctcctgccgc ggctccgggc ccgcgccctc gccgccgcgc gccccctcct
     6361 gccgcggctc cgggcccgcg ccctcgccgt cgcgcgcccc ggcctcccgc gcggcgtcgg
     6421 gcgcccccgc ctcggccgcc ggcgcgccgg cctcgcgcgc gcgcgcctcg gcttcccggg
     6481 cttccgccgc cgccgcctct tcgtcggtga tctgccgccg gaacgtcgcg acctcgatca
     6541 ccttcgcgcc gaaccggacg tgcgccaggc ggaagcggcg gccgatgatg aagcagttcc
     6601 ggaagagctt cttgacctgg taggggtgcg cgtcggtgcc gacgtcgaag tccttggggc
     6661 gccgcccgag cagcaggtcg cgcacgccgc cgccgacgag gtaggcggtg tagccggcgt
     6721 ccttcagccg gtagagcacc ttgagcgctt ccggatcgac gtcctttcgc gacagcgtgt
     6781 gctccgcgcg gggcacgatg agcggcggca tccggggaat cttactaggg gctgggggcc
     6841 gggcgccagg cgccaggcgg acagggacaa ggggcaaggg acacgggcag gcgccccgga
     6901 cacccgcgtg cggcgggcca ccaccccgga ttccggactc tggattcctt cgcctcctct
     6961 cttccttgct ccttgtccct agcgtttcgc ttccttccag ttctccccga tgcccgcctc
     7021 gacggtgagc gggacggcga ggtcggcggc gccttccatg tgccgccgca cgagggccgc
     7081 cacctcggcg gcctcgtcgc ggggcgcttc gaacagcagc tcgtcgtgca ccgtcaggat
     7141 catgcgcgcg cggcggccgc cggcgcgggc gggcctcgcc tcgagggcgc ggtggatgtc
     7201 gatcatcgcg cgcttcagga tgtcggcggc ggtcccctgg acgggcatgt tcaccgccgc
     7261 ccgctcggcg gcgctccgcg cctgcccgtt cgcgctcgtc agcccgggca cgggccgcct
     7321 gcgcccggcg agcgtcttca ccatgcccgt cgcgcgcccg tcctcgagcg tccggtcgat
     7381 gaaggcgcgc accttcggga acccggcgaa gtacgcgtcg atgaacgcct gcgcggcctg
     7441 ctgcggcacg ccgatgtccc tggccagcga gaacgcggtc ttgccgtaga gcagggcgta
     7501 gttgaccatc ttcgcccggc tccgcatctc cttgcggtcg aggccgctgt cggccccgaa
     7561 gatggcgagc gccgtgcggt cgtggatgtc ctcgccgcgc cggaacgcct cgatgagcgc
     7621 ctcgtcgccc gagaggtgcg ccagcacgcg cagctcgatc tgcgagtagt cggccgagat
     7681 caggacgcat cccgggtcgg cgacgaacgc cccgcggatc cggcgcccca gctcggtgcg
     7741 cacggggatg ttctgcaggt tgggatcgct gctgctcagg cggccggtgg cggccacggc
     7801 ctggttgaag ctcgtgtgca cgcggccggt ttcggggtgg acgagcccgg gcagggcgtc
     7861 cacgtaggtc cccttgagct tctgcatgct ccgccactcg aggatgagcc gggggatctc
     7921 gtgcgcgagc gccagctcct cgaggacctc caccgaggtg gacgcggcgc gcgtcgcgcc
     7981 ggtcttcttc agcacgggca gcttcagctt ctcgaacagg atttcggcga gctgcttcgg
     8041 cgacccgatg ttgaacgggc cgccggccag ctcgaagatc cgccggctgc gctcctccat
     8101 ctcgcgctcg agcgaggccg acagcgatgc gagtgcggcg gtgtcgatgc ggacgccggc
     8161 gcgttcgatg gccgccagga cgggcacgag gggccattcg aggccgcggt acagctcgtc
     8221 caggccttcg gccgcgagcg accggcgcag ggggccggcg agccgaagcg gcaggtcggc
     8281 gcgctcggcg gcgtaggcca gcgtggcggc ggcgggcagg ttctcggggg cggccgctct
     8341 ggcgccctta ccgtagacgt cctcttcggt gaggggcctg ccgccgaggt gctcgatgct
     8401 cagcgtctcg aggccgtgcg gcgcgcgcgt ggagtcgagg aggtagcttg cgagcatcgt
     8461 gtcgaacccg ggcccggcca ccgtcacgcc gtgccgcgcg aggacgatgg cgtcgtactt
     8521 cacgtcgtgg ccgatcttct cgacgcgctc gtcttccagg agcggcgcga gcgcgccgag
     8581 cgcggccgcg gcgtcgaggt tcgcgcactc gtcgagcgcg cggtggccca gcggcacgta
     8641 ccgcgcgcgc cgctcgccgc cggcgagcgc gatgccgacg atgcgcgcgc gcatgggggc
     8701 ggcatcgtcg ccaatgacgc gaaggccggc gcggccgccg gcgcggagcg agccgacgag
     8761 cgcggccagc tcgtccagcg actgcacgac ggcgtaatcg gttgcggcgg cggccgccgc
     8821 cggcgcgaag tcgccggtca gggtgcggaa cccgagctgc gagaacagcg cgtagcacgc
     8881 ctcgcgatcc gggccgcggt agcggagcgg ctcgaggccc aggtcgaggg gaacgtccgt
     8941 ccggatggtc accagctccc ggctcgcgcg cgccgcgtcg gcgtgggcga gcagcgcctc
     9001 gcggtaccgc ttctgcgcca cgcccgccgc gccctcgagc agcgcgtcga gcgatccgaa
     9061 ctgcgccagc aggtcgcgcg cgcccttctc gccgatgccg ggcacgccct tcacgttgtc
     9121 ggaggcgtcg cccatgatcg ccagcgcgtc gatcacccgc tcgggcggca cgccgaactt
     9181 gtcagtcacg cccgccgcgt cgtaccaggt cccctcgtcc cgcgggttga acacgcggac
     9241 gcgcccgccg acgagctgga agaagtcctt gtcgccggtg acgatcgcca cgtcgtaccc
     9301 cgccgcctcg ccctgcttcg ccagggtccc gatcacatcg tcggcctcga agcccgcggc
     9361 cgtgacgacg gggacgccga gcgcggcgca ggcctggtga atccacggga tctgatcggc
     9421 gagatccgcc ggcatcggcg cccggttggc tttgtagtcc tccgccaggc cgtcgcggaa
     9481 ggtggggccc tcgaggtcga aggcgacggc gatggcgtcg ggcctatgct ccgcgaggag
     9541 cttgcggagc atcgagacga agccgaagac ggcgttcgtc gagcggccgt cgggcccggt
     9601 caggccccgg attgcgtggt aggcgcggta catctgcgcg gagccgtcga tgaggtagag
     9661 ggtgggcatg gctggcccca tcatggcggg gacgggggcg gggggcaagg ggcaaggggc
     9721 caggcgccgg gcgccgttca gtcccgagcc cacgccgaac ggcctccctc gctgcgcgag
     9781 ccgcgtccgg ctggcccctg gcaccagttc tatgctccct ggcggtgacc cgctccccgg
     9841 cggggcaatc cggtatcatg ggcggcctat gacgtccggt ctcagggccc gccgggggct
     9901 gtgtgcggcc gcgttcgcct gcg
//
LOCUS       DAOSIF010000053         9892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6618_length_9892_cov_4.321134, whole genome shotgun sequence.
ACCESSION   DAOSIF010000053 DAOSIF010000000
VERSION     DAOSIF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_9892_cov_4.321134"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLT35_03240"
     CDS             complement(<1..462)
                     /locus_tag="PLT35_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28269.1"
                     /translation="MLGAVRAEVAWFECDTSVADLEAILAGKRTRRPVDLLFLTDHLS
                     PAHHHIDEAVFELGRRCHRFGVGAEIQTCLPAAGGTWALGHDVLLYGQAGLRPGPTGG
                     YYGLTQEIIDEIFDVCIPDGAPLPETMRLRAFCRERGIACALAHPLDGHALT"
     gene            737..1912
                     /locus_tag="PLT35_03245"
     CDS             737..1912
                     /locus_tag="PLT35_03245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28270.1"
                     /translation="MVFCDIASFYTPRGGGVSTYHNEKLAFFAARGEHRYALVAPAAA
                     DEVEPVPGGTIYWLRGFRFDANYRHLYRARPLRRILREIGPDVLEFGSPYLDYWVGSW
                     AARGLGAVRTAYYHADFPDTYVAPILRRRLGRLADPAVRGLYRYVRLVFGRLDATFVA
                     SRHIRDKLQALGLTNLRHLPLGVDTAVFSPARRSAGLRRRLGVDGTGRLLLFAGRYRA
                     DKGLDVLLAALPRLLDDPAVHVAFAGTGPLAGAVGRWASDHGRVHDLGFIADRERLAE
                     LYASADVFISPGAWETFGFGLREAVASGVPVVSAAAGAGAEFVSSSGCGLLFAPGEPA
                     DLVRATLAIGRSDAADRVRAARKALESSHTWTHVFEAYVDQCRSIAAARVPGAPLGA"
     gene            1905..2636
                     /locus_tag="PLT35_03250"
     CDS             1905..2636
                     /locus_tag="PLT35_03250"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOG28271.1"
                     /translation="MHDVAPDSLAFLADLLRDLSALRVPAVNLAIVPFFHGRGTWTSQ
                     EVRRVASAVPPGIRTEVLLHGCYHRRVGANARLPAWRRLLSSIQSAGEDEFFGLPERD
                     TAERVRGGRDAVAAAFGRVPAAFVPPAWSGGPPLRAALKGLGFSLAEDHLWLYEVQRD
                     RRLFAPVIAFATRSPWREWLSARWARAVMRAPARGRLLRFAFHPADYRSPSVRACALG
                     LVEALGRTHEWTLYSELVAPGTRRG"
     gene            complement(2597..3850)
                     /locus_tag="PLT35_03255"
     CDS             complement(2597..3850)
                     /locus_tag="PLT35_03255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28272.1"
                     /translation="MKIAVVLDHDPRLLNGASRQAGQFVQWLLGRGHQVHLVHTASGM
                     HPPPSGSGLRVSTVPSFPVRSYREYRIPVPPAAARLWLARADVNLVHSETMNPTLLAL
                     AYWMKRRAGVPMFNVLTANLPYYTAILLPRDNLARKLVYGLGKPLMNAISNRIEGTFV
                     LSDGMRDSLARRFFAIDPSRLFTWTRPLDPAAYAARREPSDVWGRLGVPRGRRVATLS
                     RLCQSKNVEFLIRAFACHIIPRDPTLHLVVAGEGPNAGQLQRLAAGLGCANIHFPGLI
                     PYDAVPAFLRDTDYFVYASLSETFGNAICEAKYAGVPVVALDDGGGVRSQIADRRTGI
                     LVASADEAEFARRFLALLGDEALREAIRREAREDVVLMHSPERVYGNLMRIYQLAAEG
                     ALPGRSEIRRILAYPRLVPGATSSL"
     gene            complement(3899..>4643)
                     /locus_tag="PLT35_03260"
     CDS             complement(3899..>4643)
                     /locus_tag="PLT35_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28273.1"
                     /translation="RNHAGLLRAAAAGDRSVLGLSGHMHRSFRLRLDKQSGGLFVEQD
                     GSPIPSPGSFERSSYFVSGPSLGHVSPRAEPPERPGYLSVAVADGRIVSVTTERLRDS
                     SQCTSRFRVRCSPGNRRCRLVRVDLESPTGGLLGVDVSFLVFSMPRRSAPGGFPYVIE
                     PEAPGAAPGADPAWIDKEDCRTLLGTARRAYLQTFRCGPGIGWAFRVAPAVRPALRAR
                     AAVVIEALIPGAEAPGTASIWWHPLWVAF"
     assembly_gap    4644..4653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4654..6002)
                     /locus_tag="PLT35_03265"
     CDS             complement(<4654..6002)
                     /locus_tag="PLT35_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG28274.1"
                     /translation="MQGDCERTHGPLAAPRFLWPSLGRPEFLGPGNLSFRAVIGARAG
                     CLDAADLASGFFLTFVPDSGRRVDLASTLLSDGFSLPDGAPLKKVLGPARLYSLYEVR
                     LTVPAEALDEAAGDRRTGLFTLGHPRVSDSFHSIAWLRHGWERFRFAFVADTHLSRAW
                     EGIEADGRTLASSAGHAGLIPSDRLKRFFSREAFAASFVNPNRMWRRFVAEANALGRR
                     GELDLVILGGDLVDFLEPGNVEHFVALTTGRAPGSLALEVPLLTVPGNHDYRRFGYRP
                     QIYPLDRWGLHDLQRDHFFREVRGEGRARLSPRDFRAVFARGPGRHPLTDYFLHVSPA
                     TDEVLMAGRSVFILLDTGPDVFRTLPRVEPRRWANYLRTIPCAWRHPASAGLTDAQAA
                     TLAAATAAAGARSVFIVFHAGLGGARLETRPHTNPATPVAEGGELAAGRRGAVAVERA
                     "
     gene            6200..8674
                     /locus_tag="PLT35_03270"
     CDS             6200..8674
                     /locus_tag="PLT35_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG28275.1"
                     /translation="MRDPAVCRRALPAVLLLFALLSPREAGASASAPSAVRGIVTAMA
                     GEVLLPGVVLTLESADATAGAGATVTTLSDDRGRFRFAGLAPGAYRLTARLPGFVAAA
                     RDVAVEAGTEVEVNVDLQIEGVSETVVVVPTHPVEIGASLGSSGRIDFTRLQAMSAGN
                     VAVENILPLVPGVIRGPSGLSVKGGRPTQSSVQVGSADLTDPSTGETDFRVPTDAIAS
                     VDVLPNPYAVEFGRFTSGLTLLEARKGGDTWRLSLASIHPRFRTARGKPWRVAGVEAF
                     RPRLWFGGRLFGGRLSIAQSLHYRYTSTDLPSRPQSERIRSERLLSFTRLDASVAPGH
                     LLTATIGVSPEKRRYATLGTFEPPEASASTRERRYDVAVSEEWAVRPATFLETLVHVK
                     RHGMWITGQGALPMEIGPERRGGNYFNDQSREAWSVQWRETLSGILQGRTGIHYYKAG
                     LDVASSAFDGTDVDRPVHVLRADGTLADRIAFEGGSPHRVRATDGAAFVQDRWQAAAR
                     LVVEAGVRVDRDGVTRSAAVSPRLGMKIDLLEGNRLTVNAGIGRFVERSPLTLGALGT
                     FSTRVVTSFAPDGTPLGPPVRYAPRIGAFGLEVPRARTWHVESDVKLTPKLSLRTALL
                     GRRGSREHVAVAEEADGIGWLSVDSRGRSSYREGVLAARYAEGSRLFASASYVRSTVR
                     ADTNAYSLFFGTIRDPVIRESVYARGDADVPHRLVAQVRSQVRAWRLASVLEWRSGLP
                     YSRLDERQSWVGPPNARRLRAFVTVDVAVERQLRFGAWKPWVGVEVTNVLGRFNPQDI
                     QRNVAAPDAGVSYNSEPRRGLIVLKF"
     gene            complement(8681..8854)
                     /locus_tag="PLT35_03275"
     CDS             complement(8681..8854)
                     /locus_tag="PLT35_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28276.1"
                     /translation="MLDGITLTAARLVAASLAVFAASGLVAAALRRALPAEWFLPLVP
                     VAHAPRGRGAVGG"
     gene            complement(8889..9119)
                     /locus_tag="PLT35_03280"
     CDS             complement(8889..9119)
                     /locus_tag="PLT35_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28277.1"
                     /translation="MFHVHQTRGQAWSPPVELLIRARACSLPILVMISFFVVARSRLG
                     DPGRTFVRFVGRRFRRAGVPCLVSTAILWTAM"
     gene            complement(9337..>9892)
                     /locus_tag="PLT35_03285"
     CDS             complement(9337..>9892)
                     /locus_tag="PLT35_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28278.1"
                     /translation="KDDGSDFTQAEQPNDPYNRRAERSYSAEHQRHRVTLTGVWELPY
                     GRGAGAAGHPALLGLFGGWTLSGTFTYRSGTAENPAVGSDVNADGNSSSDRPFIGGVM
                     AARNSYEGPDYAGLNLRLSKRFRFDRRRALLLQLEGFNVLNRTNYGGLNMTWGTALEP
                     RDTYGQYTSAGAPRQLQLGVKVEF"
BASE COUNT         1409 a         3437 c         3611 g         1425 t
ORIGIN      
        1 cgtcagcgca tgcccgtcca gggggtgcgc gagggcgcac gcaatccccc gctcgcggca
       61 gaacgcgcgc agccgcatgg tctccggcag cggcgccccg tcgggtatgc agacgtcgaa
      121 gatctcgtcg atgatctcct gggtcagccc gtagtagccg ccggtgggcc ctggacggag
      181 tcccgcctgc ccatagagca ggacatcgtg tccgagggcc caggttcctc cggccgccgg
      241 caggcaggtc tggatctcgg ctcccacgcc gaaccggtga caccggcggc cgagttcgaa
      301 gaccgcctcg tcgatgtgat ggtgcgccgg cgatagatgg tcggtcagga agagcagatc
      361 gaccggccgc ctggttcgct tcccggctag gatcgcctcg agatcggcga ccgacgtgtc
      421 gcactcgaac cacgccacct cggcgcggac cgcgcccagc atacggtcca gccattcgtt
      481 caccacgcgc gagccagggc tcgcttgcgc gttcgatccg gacgaatgaa tgtgcgtaat
      541 gactctgtag gcgaccgcca tggctggagg gccctcggcc cgccgccatc cgagggccgg
      601 agagacggga ggctctatcg atcgtcgggt accgcggcgg tccgggtcaa ggggaaaggc
      661 cggacctcga gcgctccggg gcgccgcttg agggcggcgc gatcttctga cacgatggcc
      721 tcttgcggac ggctgcatgg tcttctgtga catcgccagc ttctacacgc cacgcggggg
      781 cggcgtgtcc acctaccaca acgagaagct ggcgtttttc gccgcgcggg gcgagcaccg
      841 ctacgcgcta gtcgcgccgg cggccgccga cgaggtcgag ccggttccag gcggcacgat
      901 ctactggctc cgcggcttcc ggttcgacgc gaactaccgc cacctgtacc gcgcccgccc
      961 gctccggcgg atcctgcgcg agataggccc cgacgtgctg gagttcggca gcccctatct
     1021 ggactactgg gtgggctcgt gggcggcgcg cggcctcggc gccgtgcgca ccgcctacta
     1081 ccacgcggac ttcccggaca cgtacgtcgc tccgatcctc cgaaggcgtc tcggacggct
     1141 ggcggaccct gccgtccgcg ggctgtaccg gtacgtgcgc ctcgtgttcg ggcggctgga
     1201 cgccacgttc gtggcgtcca ggcacatccg cgacaagctc caggcgctcg gcctgaccaa
     1261 tctcaggcat ctcccgctgg gcgtggatac ggccgtcttc tctccggcgc ggaggagcgc
     1321 cggtttacgc cgccggcttg gcgtcgacgg cacgggcagg ctgctcctct tcgcgggacg
     1381 gtaccgcgcc gacaaagggc tcgacgtgct gctcgccgcc ctgccgcgcc tcctcgacga
     1441 tccggccgtg cacgttgcgt tcgcgggcac cggcccgctc gcaggcgccg tggggcgctg
     1501 ggcctcggac cacggccggg tgcacgatct cgggttcatc gcggacaggg agcggctcgc
     1561 cgaactctac gcctcggccg acgtcttcat tagccccggg gcctgggaga ccttcgggtt
     1621 cggtcttcgc gaggctgtcg cgagcggagt gccggtcgtg tcggccgcgg ccggcgcggg
     1681 agccgaattc gtctcgagtt cgggttgcgg cctgttgttc gcgcccggag aaccggccga
     1741 cctggtccgc gccacgctcg cgatcggccg gtcggacgcg gctgaccgcg tgcgcgccgc
     1801 ccggaaggcc ctggaatcca gccacacgtg gacgcacgtc ttcgaggcct atgtcgatca
     1861 gtgccgatcg atcgccgcag ccagggtgcc gggcgctcct ctcggtgcat gacgtcgcgc
     1921 cggacagcct ggccttcctg gctgacctgc tgcgcgatct gtccgccctg cgcgtgccgg
     1981 cagtgaacct cgccatcgtc cccttcttcc acggccgcgg gacatggacc agccaggagg
     2041 ttcgccgagt cgcaagtgcc gtcccgccgg gaatccggac ggaggtgctc ctgcacggct
     2101 gctatcaccg gcgcgtcggc gccaacgcgc ggctgccggc gtggcgccgg cttctgtcgt
     2161 cgattcagag cgcgggagaa gatgagttct tcggactgcc ggagcgtgac accgccgagc
     2221 gcgtccgcgg cgggcgggac gcggtggcag ccgcgttcgg ccgggtgccg gcggcgttcg
     2281 ttcccccggc gtggagcggc ggcccgccgc tgcgcgccgc cctgaagggc ctcggcttct
     2341 cgctggcgga agaccacctc tggctgtacg aggtgcagcg ggaccggcgg ctgttcgcgc
     2401 cggtcatcgc cttcgccacg cgcagcccct ggcgggaatg gctgtcggcg cgctgggcgc
     2461 gcgccgtcat gcgtgcgccg gcgcggggcc gcctgctccg gttcgcgttt cacccggcgg
     2521 actaccggtc gccgtcggtc cgcgcgtgcg cgctcggcct cgtcgaggcc ttgggccgca
     2581 cccacgaatg gacgctctac agtgagctgg tggcgccggg caccaggcgg ggataggcca
     2641 ggatccgcct gatctcggag cggcccggca gcgctccctc ggcggccagc tgatagatcc
     2701 gcatcaggtt gccgtacacc cgctcgggag agtgcatcag gacgacgtcc tcgcgcgctt
     2761 cgcggcgtat cgcctcccgc agggcctcgt cgcccagcaa cgcgaggaag cggcgagcga
     2821 actccgcttc gtcagcgctc gccacgagta tcccggtccg gcgatccgcg atctgcgacc
     2881 gcacgccgcc gccgtcatcc agggcgacga cgggaacgcc ggcgtacttg gcctcgcaga
     2941 tcgcgttccc gaacgtctcg gaaagcgacg cgtagacaaa gtagtccgtg tcgcgcagga
     3001 acgccgggac ggcgtcgtac gggatgaggc cggggaagtg gatgttggcg catcccaggc
     3061 cggcggccag tcgctgcaac tggccggcat tcgggccctc ccccgccacg accaggtgca
     3121 gcgtggggtc gcgcgggatg atgtggcagg cgaacgcccg gatgagaaac tcgacgttct
     3181 tggactggca gagccggctc agcgtcgcga cccggcggcc cctggggacg cccagcctcc
     3241 cccacacgtc cgacggctcg cgcctcgccg cgtaggcggc agggtcgagg ggccgcgtcc
     3301 aggtgaagag ccgcgagggg tcgatggcga agaaccgcct ggcgaggctg tcccgcattc
     3361 cgtcgctgag cacgaacgtg ccctcgatcc ggttggagat cgcgttcatc agcggcttgc
     3421 cgaggccgta caccagcttc cgcgccaggt tgtccctggg caggaggatg gcggtgtagt
     3481 acgggaggtt ggccgtcagc acgttgaaca tcggcacgcc cgcccgccgc ttcatccagt
     3541 acgcgagcgc cagcagcgtc gggttcatcg tctcggagtg gacgagattc acgtcggcgc
     3601 gggcgagcca gagccgggcg gccgccggcg gcaccgggat ccggtactcg cggtacgacc
     3661 tgacgggaaa cgagggcacc gtgctcacgc ggagcccgga tccggacgga ggcggatgca
     3721 ttccactcgc cgtgtgcacc aggtgcacct ggtgtccgcg cccgaggagc cactggacga
     3781 actggccggc ctggcggctg gctccgttga gcagccgcgg gtcgtgatcg aggacgaccg
     3841 cgatcttcat gcgcgccgag acatcctcag cgcttataac atgccgtcga acagagcgtc
     3901 aaaaagccac ccacagcggg tgccaccaga tgctcgccgt tcccggggcc tccgctccgg
     3961 gtatcagggc ctcgatgacc acggcggccc tggcgcggag ggccggacgg acggcaggag
     4021 cgacccgaaa cgcccatcca attcctggcc cgcacctgaa ggtctggagg taggcgcgcc
     4081 tggcggtgcc gagaagcgtc cggcagtcct ccttgtcaat ccaggccgga tcggctcccg
     4141 gcgcggcgcc aggcgcttcc ggttcaatga cgtacggaaa gccccctggc gcgctccggc
     4201 gcggcatcga gaagaccagg aagctgacat ccacgcctag gagcccgccg gtcgggctct
     4261 cgagatcgac gcggaccagc cggcaccgcc ggttgcccgg gctgcaccgc acgcgaaacc
     4321 gactcgtgca ctggctcgag tcccgcagtc gctcggtcgt gacggacacg atccggccgt
     4381 cggccacggc cacgctgagg tagccgggcc gttccggcgg ctcagcgcgc gggctcacgt
     4441 ggccgaggga ggggccgctc acgaagtacg acgagcgctc gaaagacccc ggcgacggga
     4501 tcgggcttcc gtcttgctcc acgaagagac cgcccgactg cttgtcgagg cgcagcctga
     4561 acgaccggtg catgtggccg gacaggccaa gcacgctgcg gtcgccggcg gccgccgcgc
     4621 ggaggaggcc ggcgtggttt ctgnnnnnnn nnngcgcgtt ccacggcgac tgctccgcgg
     4681 cgccccgcgg ccagttcgcc gccttcggcg acgggcgtcg cggggttcgt gtgcggacgc
     4741 gtctcgagcc gcgcgcctcc gaggcctgcg tgaaagacga tgaacacgct gcgcgcgccc
     4801 gcggctgccg tcgccgcggc gagcgtcgca gcctgggcgt cggtgagacc ggccgacgcg
     4861 ggatgtcgcc acgcgcacgg aatcgtgcgc aggtagttcg cccatcggcg aggctccacc
     4921 cgcggcagcg ttcggaacac atccgggccc gtatcgagca ggatgaacac cgagcggcct
     4981 gccatcagca cctcgtcggt cgccggactg acgtgcagga agtagtcggt gagcggatgc
     5041 cggccggggc cgcgggcgaa gacggcccgg aaatccctcg gcgacaggcg cgcgcggcct
     5101 tcgcccctga cttccctgaa gaagtgatcg cgctgcagat cgtggagccc ccagcggtcg
     5161 agcgggtaga tctgcggccg gtacccgaag cggcgatagt cgtggttgcc gggcaccgtc
     5221 aggagcggca cctcgagggc gaggcttccc ggcgcccggc ccgtcgtcaa agccacgaag
     5281 tgctccacat tgcccggctc caggaagtcg acgagatcgc ctccgaggat cacgaggtcg
     5341 agttcgcccc gccggccgag ggcgttggct tctgcgacga agcggcgcca catgcggttc
     5401 gggttgacga agctcgcggc gaaggcctcc cgcgagaaga agcgcttgag ccggtctgac
     5461 gggatcagcc ccgcgtgtcc cgcactggag gcgagggtgc gtccgtcggc ctcgatccct
     5521 tcccaggctc gcgacaggtg cgtgtcggcc acgaaggcaa aacgaaagcg ctcccagccg
     5581 tggcggagcc aggcgatgga gtggaagctg tcggacacgc ggggatggcc gagggtgaag
     5641 aggccggtgc gccggtcccc ggcggcttcg tccagcgcct ctgccgggac ggtgaggcgc
     5701 acctcgtaga gggagtacag acgcgccggg ccgagcacct tcttcagagg ggcgccgtcc
     5761 ggcagcgaga agccgtcgct gaggagcgtc gaagccaggt cgacgcgccg cccggaatcg
     5821 ggcacgaacg tcaggaaaaa cccagaggcg aggtcggccg catcgaggca cccggctcgc
     5881 gcgccgatga ccgccctgaa ggacaggttg ccggggccga ggaactcggg gcgtccgaga
     5941 ctcggccaga gaaaccgggg ggctgccagc ggcccgtgcg tccgttcaca gtcgccctgc
     6001 atgggtcaaa gccggctggc cattacacag gaacgacttc cggggtgtca agggcgtggc
     6061 ggcgggtggc gatgcggcgt cgatccgaac cgcaggccga ccggcattgc tcgtggtgcc
     6121 ttcgaaccga tattctcagg gggttctcct ccgcccgagg tccggcgcag ctcctgccgg
     6181 acgcggaagg tcgtcatcca tgcgtgaccc cgccgtctgc cgccgggcgc tccccgcggt
     6241 gctgctgctc ttcgccctgc tgtcgccgcg cgaggcggga gcgtcggcgt ctgcgccgtc
     6301 ggccgtgcgc gggatcgtca cggccatggc cggagaggtc ctcctcccgg gcgttgtcct
     6361 caccctggag tccgccgatg cgacggcagg cgcaggcgcg acggtcacca ccctgtcaga
     6421 cgatcgcggc cgtttcaggt ttgcggggct ggcccctggg gcgtatcgcc tgacggccag
     6481 gctcccgggc ttcgtggcgg cggcacgcga cgtggcggtc gaggccggga ccgaggttga
     6541 ggtgaacgtc gacctgcaaa tcgagggcgt ctccgaaacc gtcgtggtgg tcccgacgca
     6601 cccggtggag attggcgcct ccctggggtc gtcgggacgc atcgacttca cccggctcca
     6661 ggcgatgtcg gccgggaatg tcgccgtcga gaacatcctg cctctcgtgc cgggcgtcat
     6721 cagggggccg tcggggctca gcgtcaaggg cggacgcccg acgcagagca gcgtccaggt
     6781 gggctcggcg gacctgacgg acccgtccac cggcgaaacg gacttcaggg tcccgaccga
     6841 cgcgatcgcg tcggtcgatg tgctgcccaa cccgtacgcg gtcgagttcg ggcgtttcac
     6901 ctcgggcctg acgctgctcg aggcccgcaa aggcggcgac acctggcgcc tgtcgctggc
     6961 aagcatccat ccccggttcc gaaccgcgcg gggaaaaccc tggcgcgtgg ccggggtcga
     7021 ggcgttcagg ccccgcctgt ggtttggcgg acgcctgttt ggcggacggc tttcgattgc
     7081 ccagagcctg cactatcgct acacgagcac ggacttgccg agccggccgc agtccgagcg
     7141 catccggagc gagcggctgc tgtcgttcac caggctggac gcgtccgtcg caccgggcca
     7201 cctgctcacg gcgaccatcg gcgtgtcgcc cgagaagcgc cggtacgcga cgctgggaac
     7261 cttcgaaccg ccggaggcgt ccgcctcgac ccgcgagcgc cggtacgacg tggcggtctc
     7321 cgaggagtgg gccgtccggc cggccacgtt cctggagacg ctcgtgcacg tcaagcggca
     7381 cgggatgtgg atcaccgggc agggcgcgct cccgatggag atcggacccg agcgccgcgg
     7441 ggggaactac ttcaacgacc agtcccgcga ggcgtggtcc gtgcagtggc gcgagacgct
     7501 ctcgggcatc ctgcaggggc ggactggcat tcattactac aaggccggtc tcgacgtggc
     7561 gtcatcggcg ttcgacggga ccgatgtgga ccggccggtg cacgttctcc gcgcggatgg
     7621 cacgcttgcc gatcggattg cgttcgaggg cggctcgccg caccgcgtgc gggcgacgga
     7681 tggcgcggcg ttcgtccagg atcgctggca ggccgcggcg cggctcgtcg tcgaagccgg
     7741 cgtgcgcgtc gatcgggacg gtgtgacgcg ctccgccgcg gtctcgccca ggctgggcat
     7801 gaagatcgat ctgctggagg gcaaccggct gaccgtcaac gccggcatcg gccggttcgt
     7861 ggagcgcagc ccgctcacct tgggggccct cggcacgttc agcacgcgcg tcgtgacgtc
     7921 gttcgcgccg gacgggactc cgctgggccc gcccgtccgc tacgcgcctc gcatcggcgc
     7981 gttcggcctg gaggttccac gggcccggac gtggcacgtc gagtccgacg tgaagctgac
     8041 gccgaagctg tctctccgga ccgccctgct cggccgacgc gggtcccggg agcatgtggc
     8101 cgtcgcggaa gaggcggacg ggatcggctg gctgagcgtg gacagccgcg gccgctcgtc
     8161 ctaccgggag ggcgtgctgg cggcgcgcta cgcggaaggc tctcgcctgt tcgccagcgc
     8221 gtcctacgtc cgatcgacgg ttcgcgccga cacgaatgcg tattccctct tcttcggcac
     8281 gatccgggat cccgtcattc gcgagtctgt ctacgcgcgc ggcgacgccg acgtgccgca
     8341 ccgcctggtc gcgcaggtgc gaagccaggt ccgcgcgtgg cgcctggcgt cggtcctcga
     8401 atggcgcagc ggcctgccct actcgcggct cgacgagcgc cagtcctggg tcgggccgcc
     8461 caacgcccgg cgtcttcggg cattcgtcac ggtggatgtc gccgtcgaac ggcagcttcg
     8521 cttcggcgcg tggaagccgt gggtgggggt ggaagtgacg aacgtgctcg gccgcttcaa
     8581 tccccaggac atccagcgca acgtcgcggc gccggatgcg ggcgtgtcct acaactcgga
     8641 gccccgccgg ggcctcattg tcctgaaatt ctgagcgggt tcagcccccc accgcgcccc
     8701 ggccgcgcgg ggcgtgtgcg acgggcacca gcggcaggaa ccactccgcc gggagagccc
     8761 tccgcagggc cgcggcgacg aggccgcttg cggcgaagac ggcgaggctg gcggcaacca
     8821 ggcgggccgc cgtcagggta atgccgtcga gcacgaaggc ggccgccgct gagcagcggg
     8881 acgaggactt acatcgccgt ccagaggatc gccgtcgaga ccaggcacgg cacccccgca
     8941 cgccggaagc gccggcccac gaaccgcaca aacgtccggc cggggtcccc gagcctcgac
     9001 cgcgcgacca cgaagaagct gatcatgacc aagatcggca gcgagcaggc gcgcgcgcgg
     9061 atgagcagct cgaccggcgg gctccaggcc tggcccctcg tctggtgaac gtggaacaca
     9121 ggacgacgcc gaagatgccg agcgcccgga acgcgtcgag gccgttgcag cgcgcggcgg
     9181 cagggaccgg gctcgcggcg ccaggacgag ggtccggcgc cgggtcggcc tggagcgtca
     9241 agcaggcagt cctgaaaagg gaatgacagc aggaacgcgc gggcgcccgc cgccggcggc
     9301 cgggccgagg cccggccgcc gggcggcgtg gcgcggctag aactcgacct tgacgccgag
     9361 ctgcagctgg cgcggcgcgc cggccgacgt gtactggccg taggtgtcgc gcggctcgag
     9421 cgccgtgccc cacgtcatgt tcagcccgcc gtaattggtg cggttgagga cgttgaaccc
     9481 ctcgagctgc agcagcagcg cccggcggcg gtcgaaccgg aaccgcttgg acaagcggag
     9541 gttcaacccc gcgtagtccg ggccctcgta cgagttgcgc gcggccatca cgccgccgat
     9601 gaacggccgg tccgagctgc tgttcccgtc ggcgttcacg tcggacccga cggcgggatt
     9661 ctcggccgtg ccgctgcgat aggtgaacgt gccggagagc gtccatccgc caaagaggcc
     9721 gagcagcgcg gggtggcccg ctgcgccggc gccgcggccg tagggcagct cccagacgcc
     9781 ggtcagcgtc acgcggtgac gctggtgctc ggcggagtat gaccgctcgg cgcgccggtt
     9841 gtacggatcg ttcggctgct cggcctgcgt gaagtcggag ccgtcgtcct tc
//
LOCUS       DAOSIF010000054         9808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6687_length_9808_cov_4.357634, whole genome shotgun sequence.
ACCESSION   DAOSIF010000054 DAOSIF010000000
VERSION     DAOSIF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6687_length_9808_cov_4.357634"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="PLT35_03290"
     CDS             <1..1588
                     /locus_tag="PLT35_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOG28279.1"
                     /translation="DTVLRGLGFRAEDYGKPTAAFSGGWQMRIALAKLLLAKPDLLLL
                     DEPTNHLDLDARNWLEDYLHEYPHAVILVSHDRFFLDAVVDRITDIHLRTLVDYACNY
                     TQYLAERDARIERLRDAKRRQDEEIARIEMFINRFRYQATKASQVQDRIKMLDKVVPI
                     EVPPERKRVHFTFPAAAKSGRTVFDLKGVCKAYGDTRVFDGADLFIERGDRIALLGPN
                     GSGKSTLMRLLSGVEKPDAGARTLGHQVIMQYFAQDEAARLDPALTVYQTLSSGSPVH
                     MVPMIRDILGGFLFSGDDIYKRVGVLSGGERTRLAVARMLLQPSNTLLLDEPTNHLDL
                     DSKDVLLDALMAYGGTIVFVSHDRYFIDKLATKVVDVGRGRAVLYPGTYEEFHWSRSR
                     ASDAPARPAAPITTPVSRPPVGAAKPARRADPRTPQAAAPKAGRAAEYGAKKRADAEA
                     QKRARARKALHARIADLEGRITAREQEVAALEAAMSRPGFYQDREGSQELVDRHQRLM
                     WEVGDLMSQWEALQKHAEDG"
     gene            complement(1683..2789)
                     /gene="mraY"
                     /locus_tag="PLT35_03295"
     CDS             complement(1683..2789)
                     /gene="mraY"
                     /locus_tag="PLT35_03295"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG28280.1"
                     /translation="MIYLIGQLLEGGYRAWSFLRLLDYLSVRAIGAAMTALILVLIVM
                     PRFIWFLHRRGLVDHTRETGVPSAFDKAGTPAMGGVVMVGAVLAACLVWCDPTNRFVW
                     AVVAAMIWFALIGLSDDVAKWREQSGNRGMSEARKLVLQGLFAALFVAFLASPYSPLP
                     AREAGTLYVPFVKHAIVSSVWVYLPVVFLFVVLVGNAVNITDGLDGLAIVPSAFAVAT
                     LGFFAWIMGNAIWSRYLFFPLFPHVGELVVFCAAFVGAAIGFLWFNAYPAQVFMGDAG
                     SLAIGGCLAALSVLVKQEALFLILGGLFIAEAATSQVQDKIGIKWLGRRLFYRAPLHH
                     QLQHTGLAETKVVIRLWIVALILALVSIATVKLR"
     gene            complement(2786..4813)
                     /locus_tag="PLT35_03300"
     CDS             complement(2786..4813)
                     /locus_tag="PLT35_03300"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOG28281.1"
                     /translation="MTAFSRILLTGGGTAGHVNPALAIGRALGDERTAYLYVGVRGRA
                     EADVVPREGVPIRFVRASPYPGARPSLAWPGFLFDLLVGTAQALAIVRSFRPDVIIGT
                     GGFASAPTMVAAAILRRLGLTEARLYVHEQNAAPGRLNLLVGRLADRVFVAFDDTLAA
                     FPSNGVLAGYPLRRRIRRLDPEAARAALDFAVEPGRRVVFAFGGSQGARTINRAVVDA
                     LGSLLPHKDRLLVVHGTGLRRGAGALDPDADARARLAERYTEEERRAIAGFYVARPYF
                     HQIEHVYALADLVVVRGGAGTLNEVAALGLPAIVVPKTNLPGEHQVMNARALAASGGA
                     VVLYEERRLEGGEAVEALDGAVLASQILSLLDTPGRLEQMGASTRAFVRRDALDAIRR
                     SICGEAAAPPDAAAPPAGGRLLSSEALLYALEHRRASTVPAGARRPEQPAPADRAYYV
                     ARAASLAASPAWETRNLGVKLIGLLEARDKLPVLLAILGDRTPAPWYKRALGGDYAQV
                     GFIRRNALTAIARLGVVTPDVETRLSASLDDPYYEARAEAARTITALDRGLSEAGRAR
                     LIAGLIGRLGDRWLEVAAAAAEALGRVGGEADALPALLALADHRFWAVRAAGLRGLHA
                     LVERGRGGDLDALERRVRAFPLTATDFRPEFSIRTAYARLIRAIGAKRSAT"
     gene            4960..5106
                     /locus_tag="PLT35_03305"
     CDS             4960..5106
                     /locus_tag="PLT35_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28282.1"
                     /translation="MAAGAAAGARFERGECNLAALGALAEAGPATLGGQQERHEPILT
                     GYPG"
     gene            5157..5750
                     /locus_tag="PLT35_03310"
     CDS             5157..5750
                     /locus_tag="PLT35_03310"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOG28283.1"
                     /translation="MIRHPLAFALAACAALGLVPAAASAQPAAPKAQPPVLVTSCGQS
                     PGPLKITVFLKRLGVEHEYKPDATAQDVASNKFKTLIVVTGASLKGMGAAGVSIKDEL
                     ARTTALLDAAKKAGVLIVGSHIEGMARRAQGAEAGDTSDEQSIDAVCPRSQVLVIRKD
                     GDADGRFTAIAKKQGIPMIAFEKNLDIEGVMKSLFGR"
     gene            complement(5725..6978)
                     /locus_tag="PLT35_03315"
     CDS             complement(5725..6978)
                     /locus_tag="PLT35_03315"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOG28284.1"
                     /translation="MPRAGSGLGLVETRQARVCEGGLDLACGRRLDAVTVAYECYGRL
                     NAARDNAVLVCHALSGGAHAAGWHEGASKPGWWDVMIGPGKAFDTDRYFVISSNVLGG
                     CYGTTGPGSIDPATGRAYGSRFPVVTIADMVTVQRALVDALGIARLRAVAGGSMGGMQ
                     ALQWAVQYPEAVQSVIAIATSPRHSPQQIAFNEIARRAVMSDPDWLGGDYYAGEPPRA
                     GLAVARMLGHVTYLSDRGMERKFGRRRRPGARAFGFDAEFEVEAYLQHQGQAFVERFD
                     ANALLYITRAIDYFDLAPRGGTLRDAFAACGSEFLFLAFSSDWLYPPRHLEAAARAAR
                     AAGRAVTYREILSDCGHDAFLLEHEAQGPLIREFLDGSGAVRSAAPLRSADAGPARVA
                     PPGAAAWRAARPRRRARPSGRTGTS"
     gene            complement(6979..8307)
                     /locus_tag="PLT35_03320"
     CDS             complement(6979..8307)
                     /locus_tag="PLT35_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOG28285.1"
                     /translation="MPNRASSLRSATVALHGGQAPDCATGARAVPIYQTSSFVFESSD
                     HAAALFALQQPGHLYTRLGNPTTEVLERRLAELDGGVGALALASGQAAVAAAVLTLTG
                     QGQNIVSSRSLYGGTYTLFRHTLGRLGIEARFVDSSDPGSVAGAIDAGTRLVYTESIG
                     NPRNSVDDFAALAEIAHAAGVPFVVDNTVSPQIFRPFEHGADLIVYSLTKFIGGHGTS
                     IGGAIVDSGRFDWANGRFPEFTEPDPSYHGLVFWERYGTHGRAVAPGAAFITKARVQV
                     LRDLGACLSPFNAFLILQGLETLPYRMAAHCSNAARVAEWLASHPDVAWVSYPGLPDH
                     PDRARAGRYLPRGAGAIVGFGIRGGLDAARRFIDRVRLFSHLANIGDAKSLVIHPAST
                     THQQLSPEERAAAGVPDDFIRLSIGIEDADDLIADLGQAIAAAAGRAEGA"
     gene            complement(8357..9631)
                     /locus_tag="PLT35_03325"
     CDS             complement(8357..9631)
                     /locus_tag="PLT35_03325"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG28286.1"
                     /translation="MTPRRPALTVAIAGFGTVGRSVARLICERHHSRVRLEAVLTRRP
                     AAAKREAPWAPPHVTWTDDAAILLTRRTDVVVELIGGIEPARTWIASALRAGQAVVTA
                     NKQVIAEHGRELIALARARGGALGFEAAVAGAVPVIRGLQEGLAGDRVIGLAGVMNGT
                     CSFVLGRMAEAGTSLEEAVAEAQARGLAEADPADDLDGLDARAKLAILASIGFGRHVS
                     PRSIRAASIRGLDAGDIAAAASAGRVIRQVARARRPGPEARSIAAGVSAALVPRDSWL
                     GRGRGAENVVLVHGERSGTTVFAGQGAGGEATAVAVVSDVLAVALRRAVPRAWPSGPA
                     ACVRREGASLRYVRVSNLAAAGGPDPARLLEASGIGLERMLTGPIRQGGRWAAIVGPC
                     GEAALDRALRPLARAGSADRPVVRLPLFDPEI"
     gene            complement(9628..>9808)
                     /locus_tag="PLT35_03330"
     CDS             complement(9628..>9808)
                     /locus_tag="PLT35_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28287.1"
                     /translation="PGSIHVACVIDEKHLARAVSAIHSRVFERGRPPDSGRGVPADGP
                     RRRRPFGPGGTEAMA"
BASE COUNT         1463 a         3639 c         3532 g         1174 t
ORIGIN      
        1 cgacaccgtg ctccggggcc tcggcttccg cgccgaggac tacggcaagc ccacggcggc
       61 cttctcgggc ggctggcaga tgcgcatcgc cctggccaag ctcctgctcg cgaagccgga
      121 cctcctgctc ctcgacgagc cgacgaacca cctcgatctg gacgcgcgca actggctcga
      181 agactacctg cacgagtacc cgcacgccgt catcctcgtc tcgcacgacc gcttctttct
      241 cgacgcggtc gtggatcgca tcaccgacat ccacctgcgg acgctcgtgg actacgcctg
      301 caactacacg cagtacctgg ccgagcggga cgcgcggatc gagcggctgc gcgacgccaa
      361 gcggcggcag gacgaggaga tcgcccgcat cgagatgttc atcaaccggt tccgctacca
      421 ggcgacgaag gcgtcgcagg tgcaggaccg gatcaagatg ctcgacaagg tcgtgcccat
      481 cgaggtgcct cccgagcgca agcgggtgca cttcacgttc cccgcggccg cgaagagcgg
      541 gcggacggtg ttcgatctga agggcgtctg caaggcctac ggggacacgc gcgtcttcga
      601 cggcgccgat ctcttcatcg agcgcggcga tcgcatcgcg ctgctcgggc cgaacgggtc
      661 cggcaagtcc accctgatgc ggctgctgtc gggggtcgag aagcctgacg cgggcgcgcg
      721 caccctgggg caccaggtca tcatgcagta cttcgcccag gacgaggccg cgcgcctgga
      781 cccggcgctc acggtctacc agacgctctc gtcgggatcg ccggtccaca tggtcccgat
      841 gatccgcgac atcctgggcg ggttcctgtt ctcgggcgac gacatctaca agcgcgtggg
      901 cgtgctctcc ggcggcgagc gcacccgcct ggcggtggcg cgcatgctcc tgcagccgtc
      961 gaacacgctc ctcctcgacg agccgaccaa ccacctggat ctcgactcca aggacgtgct
     1021 gctcgacgcg ctcatggcct acggcgggac gatcgtgttc gtgtcgcacg accggtactt
     1081 catcgacaag ctcgcgacga aagtcgtgga cgtcggccgc gggcgcgccg tgctctaccc
     1141 gggcacgtac gaggagttcc actggagccg ttcgcgcgca tcggacgcgc cggcgcgtcc
     1201 ggcggccccg atcacgacgc ctgtctcgcg cccgcccgtc ggcgccgcga agcccgcgcg
     1261 ccgggcggac ccgcgcacgc ctcaggcggc cgcgccgaag gccggccgcg cggccgagta
     1321 cggcgccaag aaacgcgccg acgccgaagc gcagaagcgc gcgcgcgcgc gaaaggccct
     1381 ccacgcccgc atcgccgacc tcgagggccg gatcaccgcc cgcgagcagg aagtcgcggc
     1441 gctcgaggcc gcgatgtccc ggcccgggtt ctaccaggac cgcgagggct ctcaggagct
     1501 ggtcgatcgc caccagcggc tgatgtggga ggtgggcgat ctcatgagcc agtgggaggc
     1561 cctgcagaag cacgccgaag acggttagcc gggggccggc gcgcccgccc gggccgcgcc
     1621 gcccgggcga gccgcccgcc gacggccccg gcacgctcct ccgcgcggaa gcgccgcccg
     1681 gcctaccgga gcttcacggt cgcgatggac accagcgcca ggatgagggc cacgatccag
     1741 agccggatga cgaccttcgt ctcggccagt ccggtgtgct gcaactggtg gtgcagcggg
     1801 gcgcggtaga acagccggcg gcccagccac ttgatcccga tcttgtcctg cacctgcgac
     1861 gtcgcggcct cggcgatgaa caggccgccc aggatcagga agagcgcttc ctgcttgacc
     1921 agcaccgaca gcgcggccag gcatccgccg atcgccagcg acccggcgtc gcccatgaag
     1981 acctgcgccg gatacgcgtt gaaccagagg aagccgatgg ccgcgccgac aaacgccgcg
     2041 cagaacacga cgagctcgcc cacgtggggg aagagcggaa agaacaggta gcgcgaccag
     2101 atcgcgttgc ccatgatcca ggcaaagaag cccagcgtcg ccaccgcgaa cgcggacgga
     2161 acgatggcga ggccgtcgag gccgtcggtg atgttcacgg cgttcccgac cagcacgacg
     2221 aacaggaaca cgacggggag gtagacccac accgacgaca cgatggcgtg cttcacgaag
     2281 ggcacataca gcgtgcccgc ctccctggcc ggcagcggcg agtaggggct cgcgagaaac
     2341 gccacgaaga gcgccgcgaa cagcccctgc agcaccagct tccgcgcctc gctcatcccg
     2401 cggttgcccg actgctcccg ccacttggcg acatcgtccg agaggccgat gagcgcgaac
     2461 cagatcatgg ccgcaacgac agcccagacg aaccggttcg tggggtcgca ccagaccaga
     2521 caggcggcga gcacggcgcc gaccatcacc acgccgccca tcgcgggcgt cccggccttg
     2581 tcgaatgccg acggcacgcc ggtctcgcgc gtgtgatcga cgaggccgcg gcggtggagg
     2641 aaccagatga agcgcggcat cacgatgagc accaggatga gcgccgtcat cgccgcgccg
     2701 atggcccgca cggacaggta gtccaggagc cgcaggaacg accaggcgcg gtacccgccc
     2761 tcgaggagtt gcccgatgag atagatcacg tggcgctccg cttcgcaccg atggcccgga
     2821 tgaggcgcgc gtaggccgtc cggatgctga actccggccg gaagtccgtc gcagtcagcg
     2881 ggaacgccct gacgcgccgc tcgagcgcgt cgaggtcgcc gccccggccg cgctccacga
     2941 gcgcgtggag accgcgcagg ccggcggcgc gcacggccca gaaccggtga tcggcgagcg
     3001 ccagcagggc cggaagggcg tcggcttcgc ccccgacgcg ccccagggcc tcggccgccg
     3061 ctgccgccac ctcgagccat cgatcgccga ggcgtccgat gagccccgcg atgaggcgcg
     3121 ccctgcccgc ctcgctcagg ccgcggtcca gggccgtgat ggtccgcgcc gcctcggccc
     3181 gcgcctcgta gtacggatcg tcgagcgacg cgctcagccg cgtctcgacg tccggtgtca
     3241 ccacgccgag gcgcgcgatg gcggtcagcg cgttgcggcg gatgaacccg acctgcgcat
     3301 agtccccgcc cagcgcgcgc ttgtaccacg gcgccggcgt ccggtccccg aggatcgcga
     3361 gcaggaccgg cagcttgtcg cgggcctcga gcaggccgat gagcttcacg ccgaggttgc
     3421 gcgtctccca ggccgggctc gccgccagcg acgccgcgcg cgcgacgtag tacgcgcggt
     3481 cggccggcgc cggctgctcg gggcggcggg ccccggcagg cacggtggac gcgcgccggt
     3541 gctcgagggc gtagagcagc gcctcgctgg acagcagccg gccgccggct ggcggcgcgg
     3601 ccgcgtccgg cggcgcggcc gcctcgccgc agatcgatcg ccgaatggcg tcgagcgcgt
     3661 cgcgacggac gaacgcccgc gtggacgcgc ccatctgctc gagccgccca ggcgtgtcga
     3721 ggagagacag gatctgcgac gcgagcacgg cgccgtcgag cgcctcgacc gcctctcctc
     3781 cttcgaggcg gcgctcctcg tacagcacga cggcaccgcc cgaggccgcc agcgcacggg
     3841 cgttcatcac ctgatgctcg ccaggcaggt tggttttcgg cacgacgatg gccggcaggc
     3901 cgagagccgc gacctcgttc agcgtgcccg ctccaccgcg gaccacgacg aggtccgcca
     3961 gggcgtacac gtgttcgatc tggtggaagt acggccgcgc gacgtagaag cccgcgatcg
     4021 cgcggcgctc ctcctccgtg tagcgctcgg caaggcgcgc ccgcgcgtcg gcgtccgggt
     4081 cgagggcccc ggcgccgcgg cgcagcccgg tgccgtgcac cacgagcagc cggtccttgt
     4141 gcggcagcag cgatccgagc gcgtccacga cggcgcggtt gatcgtgcgc gcgccctgcg
     4201 acccgccgaa ggcgaacacg acgcgccgcc cgggttccac cgcgaagtcc agcgccgcgc
     4261 gggccgcctc cgggtccagc ctccggatgc gccggcgcag cggatacccc gccaggacgc
     4321 cgttcgaggg gaacgccgcg agcgtgtcgt cgaacgccac gaagacccgg tccgccaggc
     4381 ggccgacgag caggttcagc cggcccggtg ccgcgttctg ctcgtgcacg tacagcctcg
     4441 cctcggtcag gcccagcctc cgcaggatcg cggccgcgac catcgtcggg gccgacgcga
     4501 accccccggt gccgatgatg acgtccggtc tgaacgagcg gacgatcgcc agcgcctggg
     4561 ccgtgccgac gagcaggtcg aagaggaagc cgggccaggc aagcgacggc cgcgcgcccg
     4621 gatagggaga cgcccgaacg aaccggatcg gcacgccttc ccgggggacg acgtcggctt
     4681 cggcgcggcc gcgcacgccg acgtacaggt aggcggtgcg ctcgtcgcca agcgcgcgcc
     4741 cgatggcaag ggcgggattg acgtgaccgg ccgtgccgcc gcccgtgagc aggatgcgtg
     4801 agaacgccgt catcgcgtgc ggttcaaggg tatcaccttc gccgccggcc ggagcgcgga
     4861 ggcccctcgc ggcgcgcccg acgggacgca tgcacgacgc cgcccggcct tgcccgacat
     4921 cccccgttgc gctcgcatgc gaaggggcga taataccgag tggccgcggg cgccgccgcc
     4981 ggtgcccgct tcgagcgggg cgagtgcaac ctggccgcgc tgggagcgct ggcggaggcc
     5041 gggccggcca cgctgggcgg ccagcaggag cgtcacgagc cgatcctgac cgggtacccg
     5101 ggctgactga cttcggcggg gggccgcgcc cgccccccgc agagagggat gcgtccatga
     5161 tccgacatcc gctggcgttc gccctggccg cgtgcgcggc cctcggcctc gtcccggccg
     5221 ccgcatccgc gcagccggcc gccccgaagg cgcagccgcc cgtgctcgtg acttcgtgcg
     5281 ggcagagccc gggccccctg aagatcaccg tcttcctgaa gcgcctgggc gtcgaacacg
     5341 agtacaagcc cgatgccacg gcgcaggacg tcgcgtcgaa caagttcaag accctgatcg
     5401 tggttaccgg cgcgagcctg aagggcatgg gcgcggccgg cgtgtcgatc aaggacgagc
     5461 tggcacgcac caccgcgctc ctcgacgcgg cgaagaaggc cggcgtgctc atcgttggat
     5521 cgcacatcga gggcatggct cgccgggcgc agggcgccga ggccggcgac acgtcggacg
     5581 agcagtcgat cgacgccgtc tgcccgaggt cgcaggtgct ggtcatccgc aaggacggcg
     5641 acgcggacgg gcgcttcacg gccatcgcga agaagcaggg catcccgatg atcgccttcg
     5701 agaagaacct cgacatcgag ggcgtcatga agtccctgtt cggccgctag ggcgggcgcg
     5761 ccgccgcggc cgggccgccc gccaggcggc ggcccccggc ggcgcgacgc gcgcggggcc
     5821 ggcgtctgcg gacctcagcg gagccgcgga ccggacggcc ccggagccgt ccaggaactc
     5881 ccggatgagg gggccctggg cctcgtgttc cagcaggaac gcatcgtggc cgcagtcgct
     5941 gaggatctcc cggtacgtca cggcgcggcc ggcggcccgg gcagcccggg cggcggcttc
     6001 gaggtggcgc ggcgggtaga gccagtcgga cgagaacgcc aggaacagga actccgaccc
     6061 gcaggcggcg aaggcatcgc gcagcgtgcc gccgcgcggc gcgagatcga agtagtcgat
     6121 ggcgcgcgtg atgtacagca gcgcgttggc gtcgaaccgc tcgacgaacg cctggccctg
     6181 gtgctggagg taggcctcga cctcgaactc cgcatcgaag ccgaaggcgc gcgccccggg
     6241 ccggcggcgg cgcccgaact tccgctccat cccgcgatcg gacaggtagg tgacgtggcc
     6301 gagcatgcgg gccaccgcca gcccggcccg cggcggctcg ccggcgtagt agtcgccccc
     6361 cagccagtcc gggtcgctca tgaccgcgcg cctcgcgatt tcgttgaagg cgatctgctg
     6421 cggcgagtga cgcggcgatg tcgcgatggc gatgaccgac tgcaccgcct ccgggtactg
     6481 cactgcccac tgcagcgcct gcatgccgcc catcgagccg ccggccaccg cgcggagcct
     6541 cgcgatgccc agcgcatcga caagcgcccg ctgcacggtg accatgtcgg cgattgtcac
     6601 gaccgggaac cggctgccgt acgcgcgccc ggtggccggg tcgatggatc ccgggccggt
     6661 cgtgccgtag cagccgccga gcacgttcga cgagatcacg aagtagcgat cggtgtcgaa
     6721 cgccttgccc gggccgatca tgacgtccca ccatcccggc ttcgaggcgc cttcgtgcca
     6781 gccggcggcg tgggcgccgc ccgagagcgc gtggcacacc aggacggcgt tgtcgcgcgc
     6841 cgcgttcagc cggccgtagc attcgtaggc caccgtgacg gcgtcgagcc gacgcccgca
     6901 ggcaagatcg aggccgccct cgcacacccg cgcctgccgc gtctcgacca ggcccagtcc
     6961 gctgcccgcc cgcggcacct acgccccctc ggcccggccc gccgccgccg cgatcgcctg
     7021 cccgaggtca gcgatcaggt cgtcggcgtc ttcgatgccg atcgacaggc ggatgaagtc
     7081 gtcgggcacg ccggccgccg cgcgctcctc cggggagagc tgctggtgcg tggtcgacgc
     7141 gggatggatg accaggctct tcgcgtcgcc gatgttcgcc agatggctga acagccgcac
     7201 gcggtcgatg aagcgccgag cggcgtccag tccgcccctg atcccgaaac ccacgatcgc
     7261 gccggcgcca cgcgggagat agcgcccggc gcgcgcgcga tcgggatgat ccggcaggcc
     7321 cgggtagctc acccacgcca cgtcggggtg cgacgccagc cactcggcaa ccctggcggc
     7381 gttcgagcag tgcgcggcca tcctgtaagg cagcgtctcg aggccctgca ggatcaggaa
     7441 cgcattgaag ggcgagaggc acgcgccgag gtctcgcagc acctgcacgc gcgccttcgt
     7501 gatgaaggcg gcccccggcg cgacagcccg gccgtgcgtc ccgtaccgtt cccagaagac
     7561 gagcccgtgg taggacgggt cgggctcggt gaactccggg aaccgcccgt tcgcccagtc
     7621 gaagcggccg gaatccacga tggcgccccc gatcgaggtg ccgtggccgc cgatgaactt
     7681 cgtcagcgag tagacgatga ggtccgcgcc gtgctcgaag ggcctgaaga tctgcggcga
     7741 cacggtgttg tcgacgacga agggaacgcc tgcggcgtgc gcgatctcgg ccagcgcggc
     7801 gaagtcgtcc acgctgttcc gcgggttgcc gatcgattcc gtgtagacga ggcgcgtgcc
     7861 ggcgtcgatg gcgccggcga cgctgccggg atccgagctg tcgacgaacc gcgcctcgat
     7921 gccgaggcgt ccgagcgtgt ggcggaagag ggtgtaggtg ccgccgtaga gggagcgcga
     7981 ggacacgatg ttctggccct gtcccgtcag cgtcagcacg gccgcggcaa cggctgcctg
     8041 tcccgacgcg agcgccaggg ccccgacgcc gccgtcgagc tccgccagcc ggcgctcgag
     8101 cacctcggtc gtcgggttcc cgaggcgcgt gtagaggtgg cccggctgct gcagcgcgaa
     8161 gagggccgcc gcgtgatcgg acgattcgaa cacgaacgac gacgtctggt agatcggcac
     8221 cgcgcgcgcc ccggtggcgc aatccggagc ctggcccccg tgcagcgcga cggtggccga
     8281 acgaagcgac gaagcacggt tcggcatggg tgtctccctt ggcgtccggc gcctggcggc
     8341 accggccgac tgcgtgtcag atctccgggt cgaacagcgg caggcggact accggccggt
     8401 ccgcactgcc tgcgcgcgcg agcggcctga gggcccgatc cagggccgcc tcgccacagg
     8461 gcccgacaat ggccgcccac cgcccgccct gccggatcgg ccccgtgagc attcgctcga
     8521 gcccgatgcc ggacgcctcg agcaggcgcg ccgggtccgg cccgccggcg gccgccaggt
     8581 tcgacacgcg cacgtagcga agcgacgccc cctcgcggcg cacgcaggcg gcgggccccg
     8641 acggccacgc ccgcggaacg gcgcggcgca gcgcgacggc gagcacgtcg gacaccacgg
     8701 ccaccgccgt ggcctcgccg ccggccccct gtcccgcgaa cacggtcgtg ccgctccgct
     8761 cgccgtgcac gagcacgacg ttctcggccc cccgcccgcg ccccagccag gaatcgcgcg
     8821 gcaccagcgc ggcgctcacg cccgcggcga tgctgcgcgc ctccgggccc ggcctcctgg
     8881 cacgcgccac ctggcggatg acccgccctg cggacgccgc cgcggcgatg tcgccggcgt
     8941 cgaggccccg gatcgacgcc gcccggatgc tgcggggcga gacgtgcctc ccgaagccga
     9001 tcgacgcgag gatggcgagc ttcgcccgcg cgtcgagccc gtcgagatcg tcggcagggt
     9061 ccgcctcggc cagcccgcgc gcctgggcct cggcgacggc ctcctcgagg ctggtgcctg
     9121 cttcggccat gcgccccagc acgaagctgc aggtgccgtt catgacgccg gcgaggccga
     9181 tgacacggtc gccggcgagg ccttcctgga ggccgcggat gaccggcacg gcgccggcca
     9241 ccgccgcctc gaatccgagc gcgccgccgc gcgcccgcgc cagcgcgatc agctcccggc
     9301 catgttcagc gatgacctgc ttgttcgccg tgacgacggc ctgcccggcc cgaagcgccg
     9361 aggcaatcca cgtccgggcg ggctcgatgc cgccgatgag ttcgacgacg acgtcggtcc
     9421 gccgcgtcag caggatggcg gcatcgtcgg tccacgtgac gtgcgggggc gcccacgggg
     9481 cctcgcgctt ggccgccgcc ggacggcgcg tcagcacggc ttcgaggcgc acccgggagt
     9541 gatgccgctc gcagatcagg cgggcgaccg accggccgac cgtgccgaag ccggcaatgg
     9601 cgaccgtcag cgcggggcgc cggggcgtca cgccatcgcc tccgtgccgc ccgggccgaa
     9661 gggccggcgc cgccggggcc cgtccgccgg gacaccgcgg cccgagtccg gtggccggcc
     9721 ccgctcgaag acgcgcgagt gaatggcgct gaccgcgcgg gcgaggtgct tctcgtcgat
     9781 cacgcaggcc acgtggatcg atccggga
//
LOCUS       DAOSIF010000055         9775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6711_length_9775_cov_4.487551, whole genome shotgun sequence.
ACCESSION   DAOSIF010000055 DAOSIF010000000
VERSION     DAOSIF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9775
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6711_length_9775_cov_4.487551"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLT35_03335"
     CDS             complement(<1..1051)
                     /locus_tag="PLT35_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOG28288.1"
                     /translation="MKRPGCFASISLAAVLLGCGQGQPELTPSNPLPVELGDPYVLKA
                     SDGRYYMVGTGGVRDGFKMYSSADLRDWKDEGRVFQGNSDTSWCVASFWAPELYEHQG
                     RFYLFYSADWRENPANELENFRIGVAVSDRPTGPYTDLYNRPIFDPGYPILDANLLLE
                     GDRVFLYYSRACYKHPVETELAAWARRQGLFDEIEESWIYGVELKPDFSGVIGEPRLL
                     LRPPAAMDDRQAEWESRSVTSGEVNRRWTEGSFIFKHRGVYYLMYSANFYGGQHYAVG
                     YATSGSPLGPFVKAANNPVLQQNVRQGGIVTGTGHNCVTQSPDGKQMLCVYHGYTSRT
                     GKNRVVFIDRMDILEG"
     gene            1252..2511
                     /locus_tag="PLT35_03340"
     CDS             1252..2511
                     /locus_tag="PLT35_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG28289.1"
                     /translation="MRRRPGIVVLVFGIFFVISLLTNIQGPLLPDIIGTFQLSLGLAG
                     FLPFAFFVAYGVMSIPAGLMIQRFDEKPVLLAAFVGAFLGAALFVTRPTYPVMLFSLF
                     TIGLAMAMLQVAINPLLRVAGGEENYAFFGVMAQFFFGAASWASPYLYQYLVRQLGPQ
                     ATEGGNAVIALLQRVVDPGRAWISMYWVFTAVTLAMVIVIALARIPRVELAESERAGT
                     MRSHAGLLRSRLVLMFFLGTFSYVGLEQGVSIWISKFLQDYHQVDPQAAGAIANSWFW
                     GLQTLGCLLGLVLLRLFDARRVLVAFGLLAAGTLAAALTGPRAVSMVAFPVVGFWTSV
                     MWSIIFSLALNSVDRHHGAFAGILCSGIAGGAVVPLVVGRLGDAVGLRAAMLVIFVPI
                     AYIVSIGIWARPLVQNATIRRRKEAAA"
     gene            complement(2721..3338)
                     /locus_tag="PLT35_03345"
     CDS             complement(2721..3338)
                     /locus_tag="PLT35_03345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG28290.1"
                     /translation="MFRHTPAAAIALLLLLAPLAPWAQPTAPVVRVRFDTALGSFDVE
                     VDAARAPITAANFLKYVDGGFYDGGRVHRSARIDTQADRPVKIEVIQAGISPDRRHGS
                     YAAIPLERTSVTGITHADGAISMARSGPDSATSDFFICIGDQPSLDFGGARNPDGQGF
                     AAFGRVVSGMDVVRAIHRAPAEGETLTPPIAIRLARRVGAGAAPR"
     gene            3582..5741
                     /locus_tag="PLT35_03350"
     CDS             3582..5741
                     /locus_tag="PLT35_03350"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOG28291.1"
                     /translation="MRRKVGTGGIPLGLLAAGLFIGIPLAVEAAQAPPPNGNGRARTI
                     ETKPVGSPPRIDGRLDDECWQAIEPASGFVQYDPANGSPASEETLVWTVYDRDHLYFA
                     FHVRDSQPDRIWAELTPRNGYWQNDSITIILDTYNDRRTSIEFTVNALGVQSNSVETI
                     WRSQAVRLPDGWSAEVALPFKSLRFSNAGDQVWGVNFSRYIKRLNERDYWAPVDRDRP
                     LLLQMGDLRGLADVRPGRNVEFFPYAGTRASRWAGEADDKAALGLDVKYGIRSNLILD
                     LTASPDFSEVESDPFIYQLAPYENYLREHRPFFTEGSQYFQSGGSSGFGFGPPGFSLF
                     YSRRIADPKFAAKLTGKAGAYSFGLLGAANGDASGGAEPFTAFRVKRDVLGSSQVGLF
                     VTGAGEGDRANRNVSADYQFRLGGIYSISGSHALTFNAGGPAGDNAVHSFSASRYPDA
                     GLSASFSFNRVEKNVATRTGFVGRTDVQTTMGGAGYGWRLREGAVKHVSIDATGSVVH
                     DTDGGLRGQSLGASCWLSLRPDVSVSLMTSAGRSQYQVLGVRGLQWAGRFMPARDVWA
                     GLFWSRPGWLKSIHANASWARTGVYTGGFAGVEPGRSTSVEGSLTFRPRSDIEIETGA
                     DYIRQVADRAGAAGFSGLTYEASLHYQVTRALFLSGRLRGESREDQYHVDLVAGYYFG
                     AGNVAQVAYKRGSRLELGGLREQGYSITAKVSYLLRL"
     gene            5812..6489
                     /locus_tag="PLT35_03355"
     CDS             5812..6489
                     /locus_tag="PLT35_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG28292.1"
                     /translation="MRILVVEDDRKLAAFLVKGLREEAFAVDACHDGAEALALAGSTP
                     YDLVILDLMLPRRDGFSVCRELRARRDFVPVLMLTARDAVEDRVAGLSEGADDYLVKP
                     FAFSELLARIRALLRRTQDYKASVLRAADLELSPFSRVVTRGGRVVPLTGREYALLEY
                     LMRNKGRVVTPTMILEHVWDMNYDGLSNIVNVYINHLRKKVDGHGGRRLIRTLRGQGY
                     CLDDPQA"
     gene            6473..7882
                     /locus_tag="PLT35_03360"
     CDS             6473..7882
                     /locus_tag="PLT35_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28293.1"
                     /translation="MTLRPKSLAVTLALWYVAVLTVLIAAAGFFVYRTFESRLLAEAD
                     RTARSIAGEITRDWGFTRGLSWAQAIDAGERRHEGLGVTALLVALDESGEPSSERTVG
                     SGRPRPDGLLLDRATYASVEHADENGRLLTLRRGGESFRVALFDMRGPYVLQVSLPLA
                     ETESRMRHLALLLGAGGAALLALASIGGRVIVQRALRPVNDVVAAARALSADDLSLRI
                     DGGGRQDEIGELAATFNAMLDRLEASVARMKQFTADVSHELRTPLTVVRGEIDVALRR
                     PRAAGDYRATLESVQEEMRTMSVLVEDLLFLSRLDARPGRPASEAALDEVALRAFERL
                     EPLARERKIGVDLDGIRPAAVRGDERLLERLVSNLLDNAFRHTPEGGRVALTVTPAGE
                     AGGASLAVSDTGAGIPAEAIPRLFERFFVVDPSRSRESGGTGLGLAIVKSIADLHGAA
                     VRIDSRVGEGTTVEVAFAA"
     gene            8179..9687
                     /locus_tag="PLT35_03365"
     CDS             8179..9687
                     /locus_tag="PLT35_03365"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOG28294.1"
                     /translation="MDVVYLAVPALLIAIALAAVWLDRWSVPVILIALGAGILFGSDV
                     LGLWHFDDIGLTNRIANLALVFILFHGGFTTSRANFRAVALPAGGLATWGVVLTAAAT
                     FAVLHGVFRWPLHKALLLAAIVSSTDAAAIFSILRRQSLPPRLASTVEIESAANDPMA
                     ILLTMAVIEGITAAHATWQMSVLDFAWKFTAGPFCGWSLAKASLSLFNRLKSQDRGHY
                     YVLSLGVILLIYGLTEMIKASGMLAVFVAGYLMGNRPFVYKQGVTNFSSAISTIGNVG
                     LFALMGLQVFPHQWGPIWLDGVLLFLVLTLFARPFAVWLGTAGMGLGWRNKAFVSWAG
                     LRGSVPIVLATYPAAAGLPIGQEIFNLVFFAVLLSIAIQGSTLGGFARCLGLTCPTRP
                     KPLFDLELFALAESDFHLLVVDLPDPQGAVGPAIADLRLPAGAVIILITRGMDIVVPK
                     GSTHLLGWDRVTVLAHAKDHETARAALLNAFSAAVPQAGAKAGSGTSVEATS"
BASE COUNT         1331 a         3432 c         3451 g         1561 t
ORIGIN      
        1 ccccctcgag gatgtccatc cggtcgatga acaccacgcg gttcttcccg gtccttgacg
       61 tgtacccgtg gtacacgcac agcatctgct tcccgtccgg cgattgcgtc acgcagttgt
      121 gcccggtgcc ggtcacgatg ccgccctgcc ggacgttctg ttgaaggacc gggttgttgg
      181 ctgccttgac gaaaggcccc agcgggctgc cggacgtggc gtagcccacc gcgtagtgct
      241 gcccgccgta gaagttggcc gagtacatca ggtagtacac gccgcgatgc ttgaagatga
      301 acgagccttc cgtccagcgt cggttcacct caccggacgt caccgaccgg ctctcccact
      361 ccgcctgccg gtcgtccatg gccgccggag gccgaaggag cagccgcggc tcgccgatga
      421 cgcccgagaa gtcgggtttc agctccacgc cgtaaatcca gctctcctcg atttcgtcga
      481 acaacccctg ccggcgcgcc cacgccgcca gttcggtctc cacgggatgc ttgtagcagg
      541 ctctcgagta gtacagaaac accctgtccc cctcgaggag caggttggcg tcgaggatcg
      601 gatagccggg atcgaagatg ggccgattgt agaggtcggt gtacggtccg gtcggccggt
      661 cggagacggc cactccgatc ctgaagttct ccagctcgtt ggcgggattc tcccgccagt
      721 cggcgctgta gaacaggtag aacctgccct ggtgttcgta cagctctggc gcccagaaac
      781 tggccacgca ccacgacgtg tcgctgttgc cctgaaagac gcgtccctcg tctttccagt
      841 cccgcaagtc ggccgacgag tacatcttga acccgtcgcg gaccccgccg gtccccacca
      901 tgtagtaccg cccgtcggac gctttcagga cataggggtc gccgagctcg acggggaggg
      961 gattgctcgg cgtcagctcc ggctggccct gcccgcagcc cagaaggacc gccgcaaggg
     1021 agatcgaggc gaagcaaccc ggtctcttca tcgtcagcgc gcgcctccgg ttgccgcggc
     1081 tcctccgtga gcgcgccgcc gccatgcgcg ggcgcctcgc cggcgggatc gccgcgggaa
     1141 tgacgtagaa tcgctgctgg accggccgcg ggccatgcgt ccgcggccgg ccggcgtcat
     1201 gacgcgtcca gagcgagcat acaccgccat cttgaagagg agggcccgct gatgcgccgt
     1261 cgcccgggga tagtcgtcct cgtgttcggc atcttcttcg tgatctcgct cctgacgaac
     1321 atccaggggc cgctgctccc cgacatcatc ggcaccttcc agttgagcct gggcttggcc
     1381 ggttttctgc cgttcgcctt cttcgtggcc tacggcgtca tgtcgattcc ggcgggcctg
     1441 atgatccagc gcttcgacga gaagccggtg ctcctggcgg cgttcgtcgg cgcgttcctg
     1501 ggcgccgccc tgttcgtcac gcggccgacc tacccggtga tgctgttctc gctgttcacc
     1561 atcggcctgg ccatggcgat gctgcaggtg gccatcaacc cgctcctgcg cgtcgcgggc
     1621 ggcgaggaga actacgcctt cttcggcgtg atggcccagt tcttcttcgg cgccgcgtcc
     1681 tgggcgagcc cgtatctcta ccagtacctg gtccgccagc tggggccgca ggcgacggag
     1741 gggggcaacg cggtcatcgc gctgctgcag cgcgtcgtcg atccgggccg ggcctggatc
     1801 agcatgtact gggtcttcac ggcggtgacc ctcgccatgg tgatcgtcat cgcgctggcg
     1861 cgcattccgc gcgtcgagct ggccgaatcg gagagggccg ggacgatgcg ttcgcacgca
     1921 ggcctgctgc gaagccgcct ggtgctgatg ttcttccttg gcaccttctc gtacgtgggg
     1981 ctcgagcagg gcgtctcgat ctggatttcg aagttcctgc aggactatca ccaggtcgac
     2041 ccgcaggcgg ccggcgccat cgccaactca tggttctggg ggctgcagac gctggggtgc
     2101 ctgctgggcc tcgtgctgct gaggctcttc gacgcgcggc gggttctggt cgccttcggc
     2161 ctcctggccg cgggcacgct ggccgcggcg ctgaccggac cgcgcgccgt ctcgatggtg
     2221 gcgtttcccg tggtcggctt ctggacctcg gtgatgtggt cgatcatctt ctcgctggcg
     2281 ctgaactcgg tggaccggca ccacggcgcg ttcgcgggca tcctctgcag cggcatcgcc
     2341 ggcggcgccg tcgtcccgct cgtggtggga cggctgggcg acgccgtcgg cctgcgcgcc
     2401 gcgatgctcg tcatcttcgt cccgatcgcc tacatcgtgt cgatcggcat ctgggcgcgg
     2461 ccgctggtgc agaacgccac catccgccgg cgcaaggagg ccgccgcctg acagggccgc
     2521 cgcgcgggtc tgaccggcgg gagcacgccg tcgaggccgc cgaccggcgg gccgctgacg
     2581 ccggcgcgcg cgccaggggg cgtgccgccc gagacgactg cttcgccgac atcctcgacc
     2641 gcgtcctcaa gatgggcgac agcccgccgg ccggatacct gccgagcgga gcgccggacg
     2701 atgcgggaga cggcggccgg tcatcgcggc gcggcgcccg cgccgacccg ccgggccagg
     2761 cggatggcga tcggaggcgt cagcgtctcg ccctccgccg gagcccggtg aatcgcgcgg
     2821 accacgtcca tccccgacac gacgcggccg aacgcggcga agccctggcc gtcggggttc
     2881 cgggcgccgc cgaagtcgag cgacggctgg tcaccgatgc aaatgaagaa gtcggacgtc
     2941 gccgagtccg gcccggaccg ggccatcgag atcgcgccgt ccgcgtgcgt aatcccggtg
     3001 acgctcgtgc gctcgagggg gatggccgcg taactcccat gccggcgatc gggagagatg
     3061 ccggcctgga tgacctcgat cttcaccggc ctgtcggcct gcgtgtcgat gcgcgccgac
     3121 cgatgaacgc ggccgccgtc gtagaagcct ccgtccacgt acttcaggaa gttcgcggcg
     3181 gtgatcggcg cccgggcggc atcgacctcc acgtcgaagg agcccagcgc ggtgtcgaag
     3241 cggacgcgga cgacgggcgc cgtcggctgc gcccaggggg cgagcggcgc cagcaggagc
     3301 aacagcgcaa tggcggcggc cggggtgtgc ctgaacatag ggacagtcta tcgccgttcg
     3361 ggccccgctc ctcggcggca gcctcgccgg caccgcgccg cggggccttg ggcgcgccgc
     3421 agccatcccg ctggcgacgg aggctccagc ccgccggcgc cgcctgccgc ccgggcggcg
     3481 gggctcgccg ccggcgcccc atgaacgttc cttcatctgg cgttcaccgc cagttcatcc
     3541 gccgtggggc acactggcgc tggtgacgtg gaggcgtgcg aatgcggcgt aaggttggca
     3601 caggcggcat cccgctcggg ctgctggccg ccggtctctt catcgggatc ccgcttgccg
     3661 tggaggcggc gcaagcgccg cctccgaacg gcaacgggcg ggcacggacc atcgagacga
     3721 aaccggtggg ctcgcccccg cgcatcgacg gccggctgga cgacgaatgc tggcaggcga
     3781 tcgagcctgc gtccggcttc gtgcagtacg acccggccaa cggctccccg gcctcggagg
     3841 agacgctggt gtggaccgtc tacgatcgcg atcacctgta cttcgccttc cacgtgcgcg
     3901 actcgcagcc ggaccgcatc tgggcagagt tgacaccgcg caacggctac tggcagaacg
     3961 actcgatcac gatcatcctc gacacctaca acgaccgccg gaccagcatc gagttcacgg
     4021 tcaacgccct cggcgtgcag agcaactcgg tcgagaccat ctggcggtcg caggccgtcc
     4081 ggctcccgga cgggtggagc gcagaggtgg ccctcccgtt caagtcgctg cggttctcga
     4141 acgccggcga ccaggtctgg ggcgtcaact tctcccgcta catcaaacgg ctcaacgagc
     4201 gcgactactg ggcgcccgtc gatcgcgaca ggccgctgct gctgcagatg ggcgacctgc
     4261 gcgggctggc cgacgtgcgg cccgggcgga acgtcgagtt cttcccgtac gccggcacgc
     4321 gcgcgtcgcg gtgggccggc gaggcggacg acaaggcggc gctgggcctc gacgtcaagt
     4381 acggcatccg gtccaacctg atcctggatc tgacggccag cccggacttc agcgaagtcg
     4441 agtcggatcc gttcatctac cagctcgcgc cgtacgagaa ctacctgagg gagcaccggc
     4501 cgttcttcac cgagggcagc cagtacttcc agtccggcgg cagcagcggg ttcggtttcg
     4561 ggcctccggg gttctcgctc ttctactcgc ggcgcatcgc cgatccgaag ttcgcggcga
     4621 agctgacggg gaaggcgggc gcctactcgt tcggccttct cggcgcggcg aacggcgacg
     4681 cgtcgggcgg cgcggagccc ttcaccgcct tccgggtgaa gcgcgacgtg ctgggctcct
     4741 cccaggtcgg gctgttcgtc acgggtgccg gcgagggcga ccgggccaac cgcaatgtgt
     4801 cggccgacta ccagtttcgc ctcggcggca tctacagcat cagcgggtcg cacgccctca
     4861 cgttcaacgc gggcggcccg gcgggcgaca acgccgtcca ctccttcagt gcgagccgct
     4921 atccggacgc gggcctgagc gcgtcgttct ccttcaaccg cgtcgagaag aacgtggcga
     4981 cccgcaccgg cttcgtcggc cgcaccgacg tccagacgac gatgggcggc gccgggtacg
     5041 gatggcgcct gcgcgagggg gcggtgaagc acgtgtcgat cgacgccacc gggagcgtcg
     5101 tgcacgacac cgacgggggg cttcgcgggc agtcgctcgg agcgagctgc tggctgtcgc
     5161 tgcgccccga cgtcagcgtg tcgctgatga cgagcgcggg acgcagccag taccaggtcc
     5221 tgggcgtgcg ggggctccag tgggccggcc gcttcatgcc cgcgcgcgac gtgtgggcgg
     5281 gcctgttctg gtcgcggcca ggctggctga aaagcatcca tgcgaacgcg agctgggcgc
     5341 ggacgggcgt gtacacgggc gggttcgccg gcgtcgagcc gggccgatcg acgagcgtgg
     5401 agggatcgct caccttccgg ccgcgcagcg acatcgagat cgaaacgggc gccgactaca
     5461 tccgccaggt ggccgaccgc gcgggcgcgg caggcttctc gggcctgacc tacgaagcca
     5521 gcctgcacta ccaggtgacg cgcgcgctct tcctcagcgg ccggctgcgc ggcgagtcgc
     5581 gcgaggacca gtaccacgtc gacctggtgg ccggctacta cttcggcgcg ggtaacgtgg
     5641 cccaggtggc ctacaagagg ggcagccgcc tggagctggg ggggcttcgc gagcaggggt
     5701 actccatcac ggcgaaggtt tcgtacctgc tgcggctgta gcgcgtcgga gcgcgcccgg
     5761 gccaggccgc cgcgccgcgg ggtggccccg gcgccgcgcg tataatgcgc catgcgaata
     5821 ctggtggtcg aggacgaccg gaagctcgcg gccttcctcg tgaagggcct gcgcgaggag
     5881 gcgttcgccg tcgatgcctg ccacgacggg gccgaggcgc tcgcgctggc cggctcgacg
     5941 ccgtacgacc tcgtgatcct cgacctgatg ctgccgcgcc gggacggctt ctcggtctgc
     6001 cgggagctgc gcgcgcgccg cgacttcgtc cccgtgctca tgctgaccgc ccgggacgcc
     6061 gtcgaggacc gcgtggccgg gctgtcggag ggcgccgacg actacctggt caagcccttc
     6121 gccttctcgg agctgctggc gcgcatccgc gcgctgctcc ggcgcaccca ggactacaag
     6181 gcgagcgtgc tccgggcggc cgacctcgag ctgtcgccgt tcagccgcgt ggtgacgcgc
     6241 ggcggccggg tcgtcccgct gacggggcgg gagtacgcgc tgctcgagta cctcatgcgc
     6301 aacaaggggc gcgtcgtcac cccgacgatg atcctcgagc acgtctggga catgaactac
     6361 gacggcctga gcaacatcgt caacgtgtac atcaaccacc tgcggaagaa agtggacggc
     6421 cacggcggcc gccggctgat ccggacgctt cgcggacagg gctactgcct cgatgaccct
     6481 caggcctaag tcgctcgccg tcactctggc gctgtggtac gtggcggtgc tcacggtgct
     6541 catcgcggcg gcagggttct tcgtgtaccg cacgttcgag tcgcgcctgc tggccgaggc
     6601 ggaccggacg gcgcggtcca tcgccgggga aatcacgcgc gactggggct tcacgcgggg
     6661 cctgagctgg gcgcaggcca tcgacgccgg cgagcggcgc cacgagggcc tgggcgtgac
     6721 ggcgctgctg gtcgccctcg acgagtcggg cgaaccatcc tcggagcgga cggtgggatc
     6781 gggccggccg cggcccgacg gactgctgct cgaccgggcg acgtacgcca gcgtggagca
     6841 cgcggacgag aacggccgcc tgctcaccct gcgccgcggc ggcgagtcgt ttcgcgtcgc
     6901 gctgttcgac atgcgcgggc cgtacgtcct gcaggtgagc ctgccgctcg ccgagaccga
     6961 gtcgcgcatg cggcacctgg cgctcctgct gggcgccggc ggcgcggcgc tgctggcgct
     7021 ggcgtcgatc ggcggccgcg tgatcgtcca gcgggccctg cggccggtga acgacgtcgt
     7081 cgccgcggcg cgggcgctgt cggccgacga cctctcgctc cggatcgacg gcggcgggcg
     7141 ccaggacgag atcggcgagc tggcggccac cttcaacgcg atgctcgacc ggctcgaggc
     7201 gtcggtggcg aggatgaagc agttcacggc cgacgtgtcg cacgagctgc gcacgccgct
     7261 gaccgtcgtc cgcggcgaaa tcgacgtggc gctgcggcgg ccccgcgcgg cgggagacta
     7321 ccgcgcgacg ctcgagagcg tgcaggaaga gatgcgcacg atgagcgtgc tggtcgaaga
     7381 cctgctcttc ctgtcgcgcc tggatgcgcg gccgggcagg ccggcgtccg aggcggccct
     7441 cgatgaagtg gccctcaggg cgttcgagcg cctcgagccg ctggcgcgcg agcggaagat
     7501 cggcgtcgat ctcgacggga tccggccggc agccgtgcgc ggcgacgagc gcctgctcga
     7561 gcgcctggtc tcgaacctgc tcgacaacgc cttccgccat acgccggaag gggggcgcgt
     7621 cgcgctgacg gtgacgccgg ccggcgaggc gggaggcgcg tcgctcgcgg tcagcgacac
     7681 gggcgccggc atcccggccg aggccatccc ccggctgttc gaacgcttct tcgtcgtcga
     7741 tccctcgcgg agccgggaga gcggcggcac gggcctcggt ctggcgatcg tcaagtcgat
     7801 cgccgacctg cacggtgcgg cggtgcgcat cgacagccgt gtcggggagg gcacaacggt
     7861 ggaggtggcc ttcgccgcgt gagccgcgcc ggcaggcgcg ggacggcctc gacgaaagcc
     7921 tacgcccgca ggccgggacg tcgcgaacct ccactatgat ggtagctgca cccggttccg
     7981 gcagaggccg agccgccccc cggccggacc gtcggcgagg cgcacgccgc gccgccgccc
     8041 ccctgcccgc gcccgcgatg cgccgccccc ggcgggcggg gccgccgcat ccggggcccg
     8101 agaggacgca ggacgccctg atcatgcgtc ctgtcgccgt tcgtcagcga cgcgtgggaa
     8161 agacgagtcg tggggctcat ggacgttgtc tatctcgccg taccggccct gttgatcgcg
     8221 atcgccctgg ctgccgtctg gctggaccgc tggagcgtcc cggtcatcct gatcgccctc
     8281 ggtgcgggca ttctcttcgg cagcgatgtc cttgggctct ggcacttcga cgacatcggg
     8341 ctgaccaacc ggatcgcgaa cctggccctg gtgttcatcc tgtttcacgg cggcttcacc
     8401 acaagccgcg cgaacttccg cgccgtggcc cttccggcag gcggccttgc cacctggggc
     8461 gtcgtcctga cggccgcggc gaccttcgcg gttttgcacg gggtgttccg ctggccgctg
     8521 cacaaggcgc tcctgctcgc cgccatcgtc tcgtccaccg acgcggcggc gatcttctcg
     8581 attctccgcc ggcagtccct gccgccgagg ctggcgtcga cggtcgagat cgaaagcgcg
     8641 gccaacgacc cgatggcgat cctgctcacc atggcggtca tcgaggggat caccgccgcc
     8701 cacgcgacgt ggcagatgtc cgtgctggac ttcgcctgga agttcacggc cggaccgttc
     8761 tgcggctggt ccctcgccaa ggcgtcgctg tcgctcttca atcggctcaa gtcgcaggac
     8821 cgcggccact actacgtgct gtcgctgggg gtcatcctgc tgatctacgg cctgaccgag
     8881 atgatcaagg ccagcgggat gctcgcggtg ttcgtggcgg gctacctgat ggggaaccgc
     8941 ccgttcgtct acaaacaggg cgtaaccaac ttctcgtccg ccatctccac catcggcaac
     9001 gtcggcctgt tcgccctcat ggggctccag gtcttccccc accagtgggg gcccatctgg
     9061 ctggacggcg tgctcctgtt cctcgtcctg accctgttcg cgcgcccgtt cgcggtgtgg
     9121 ctgggcaccg cgggcatggg cctcggctgg aggaacaagg cgttcgtctc ctgggccggc
     9181 cttcgcggct cggtccccat cgtcctggcc acctatccgg ccgcggccgg gctccccatc
     9241 ggccaggaga tcttcaatct cgtgttcttc gccgtgctcc tgtcgatcgc catccaggga
     9301 tccacgctcg gagggttcgc gcgatgcctc ggcctcacct gccccacgcg gcccaagccc
     9361 ctgttcgacc tggagctgtt cgccctggcc gagagcgact tccacctgct ggtggtcgat
     9421 ctgccggacc cgcagggcgc ggtgggccct gccatcgctg acttgcggct tcccgccgga
     9481 gcggtgatca tcctcatcac gcgaggcatg gacatcgtgg ttcccaaagg ctcgacgcat
     9541 ctgctgggat gggatcgcgt caccgtgctc gcccatgcca aggaccacga gaccgcccgt
     9601 gctgcgctgc tgaatgcctt ctccgcggcg gtgccgcagg ccggcgcgaa ggcaggctct
     9661 ggcacgagcg tcgaggcaac gtcctgagag ccgccgcgcg agcaccgcgc tccttgtggt
     9721 ataaggtgcg cgcccccgtg gaccggatcg gcgtcttgca cattcggcgg aggaa
//
LOCUS       DAOSIF010000056         9688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6785_length_9688_cov_4.393439, whole genome shotgun sequence.
ACCESSION   DAOSIF010000056 DAOSIF010000000
VERSION     DAOSIF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6785_length_9688_cov_4.393439"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="PLT35_03370"
     CDS             complement(<1..1428)
                     /locus_tag="PLT35_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28295.1"
                     /translation="MKSVFRRIASPTAAAAALVALAGIACSRQAAPKLDYPQTKTVDH
                     VDTYHGVAVADPYRWLEDDNSAETAAWVEAQNKVTFGYLEQIPYRAAVKARLEALYNY
                     PKYTAPRRKGDRFYFSKNDGLQNQSVLYVQQGLDGVPEVLIDPNTWSADGTSRLTAFA
                     LSKDGKYAVVGVSRAGSDWQEYGVMDVASRTMLGDRIEWVKVSGASWRGDGFYYSRYP
                     APAKGRELSSANENHQVYYHKVGTPQSADELVFEDAANPQRFHRVDTTEDERFAVLDV
                     SDRGKGKDGNALFVRDLARKDKAFRPLIGTIGDDTFSVIDNVGDKLLVRTNRGAPNGR
                     VVLIDPTRPEEPNWVTVLAERPEPLQGASTAGGKLFATYLKDVATVAEVHALDGALEH
                     VIDLPGLGTAGGFGGERDDAFVFYTFASFNVPPTIYRYDIATKASTVFRAPEIPGFDP
                     SAFETKQVFFTSRDGTRVPMFVVHRK"
     gene            complement(1735..2124)
                     /gene="apaG"
                     /locus_tag="PLT35_03375"
     CDS             complement(1735..2124)
                     /gene="apaG"
                     /locus_tag="PLT35_03375"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOG28296.1"
                     /translation="MPTSEAVTRGIRVHAESGYAPERSNPARRQWFFPYTITITNESD
                     VTVQLLARHWIITDGDNRVEEVHGPGVVGEQPVLRAGESHTYTSGCPLGTPFGMMKGS
                     YRMVTEGGERFDATVAPFALSEPYTVH"
     gene            2323..4350
                     /locus_tag="PLT35_03380"
     CDS             2323..4350
                     /locus_tag="PLT35_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOG28297.1"
                     /translation="MAPSRSPTRSRRAFAALCLATLGVVYGDIGTSPLYTISEIFFGP
                     NRIPYTPQNALGATSLVLWILTLSVTLKYVLLVLRAGYENQGGTFALLALIQRSTSSA
                     RRTGAAPLLGGLSLALVLAAALLFGEGIITPAISVLSAVEGLKIATGAFDPVVVPATA
                     LILLALFSIQSRGTARIGATFGPIIALWFVTIAGLGVTHILRHPAVLGAIDPRNAAAF
                     LWANGVPGSVAVLGSVLLAVTGCEALFADMGHFGARPIRLTWFSLVYPALMLNYLGQG
                     AYAYGGGPVEGGHLFYALVPRPLLLPMVALATLATVIASQALISGAFSLARQAIALGL
                     FPRLEIVHTSGAHEGQIYVPAINRALLVSCVFLVIGFGSSSSLAAAYGFAVSGVMLVT
                     SFAMMTIAVRAWGWPPCLAVPLFGFFAAIEAVFFFSASLKILHGAWVPLAVGLLAFSV
                     MTTWQWGRRRVAGVYAELAPRCETVRRLIELKRHPSVPQLPRSVVVMSSRPVLDLDER
                     IPPALHLFWVRLGALPKHVVLLTVRQIPVPNARAVGAPRTEAVTFFSDPRLGSVVGLQ
                     STYGYMETPDVRRALVEAKELKKIKVPGDPRKWLVLVGQENVTETKVGALVRLRLSFF
                     RTMLRNSVPAHLYFGLGSDSLVTTETVHLAAAAFDEDDVSGFVPEGPERAV"
     gene            complement(4388..5452)
                     /locus_tag="PLT35_03385"
     CDS             complement(4388..5452)
                     /locus_tag="PLT35_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG28298.1"
                     /translation="MIAFVQDDRFRKLFLVLIVAGISFLFLAMIRSFLMTLLLAAVLT
                     GLMYPLYGWLLRRMRGRRTLASLLTLVVAVVAVFGPLITIAGIVVQQAISLTTNAKGM
                     IQPYLDDPELLRSQLALIPGSARIEPILPQIAERGAQVVSSLGAFLVKQVSAATSGTV
                     VFLFHAAILLYAMFFFFVHGERYLRAVLSYLPFSDEDSQRLTARFASVTKATVKGTLL
                     IGVIQGTINGLAFWIVDLPAPTFWGVVMVVLSVVPAIGGALVWVPAAILLALTGRIGE
                     AITLTLICAGVSGSVDNLLRPRLVGRDTQMPDLVILISTLGGIGFFGAPGFIVGPLVA
                     AFFITLWQILAQSYRPAAKP"
     gene            5515..6111
                     /locus_tag="PLT35_03390"
     CDS             5515..6111
                     /locus_tag="PLT35_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28299.1"
                     /translation="MAEDTPAPVVEAPEWVAAEMPPGYQTRRWEIQRMTAELDAMDRV
                     ARVLWSTGEPLKDAVAAAFTALRCEVEAAAAPGGPLVARLGDSRRMLVVVAGAAGPIP
                     KSDESLARAFAAIQSAGTGDRVVFVATSDPASPPAGRPDPLQPDALAVLERIGVTVVA
                     ATTLFRLWRLAGEDPPKGGKALERLHAQEGGPFSLPAR"
     gene            6367..6855
                     /locus_tag="PLT35_03395"
     CDS             6367..6855
                     /locus_tag="PLT35_03395"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOG28300.1"
                     /translation="MHQLHFPKRFEHTHPPVRSVNDIETEQLTLGQRAADRVAAIVGS
                     WRFIGLQSLVLTGWACVNVVAWTHHWDPYPFILMNLFLSLQAAYTAPMLMMSQNRMAA
                     RDRLEAHNDYLINRKAEEEVRAVLDHLSAQDEALTKIVESLVGTLEAGSRPPGPGAAA
                     PR"
     gene            7159..8889
                     /gene="ggt"
                     /locus_tag="PLT35_03400"
     CDS             7159..8889
                     /gene="ggt"
                     /locus_tag="PLT35_03400"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOG28301.1"
                     /translation="MPRPGACRRRPAIVSVPSAVAAALLLLLQARTPAQTPLNLYGRG
                     ASGANGVVAAAKPDASQAGIAILKMGGNAVDAAVATGFALGVLEPNASGIGGGGFMLI
                     KLASMQEAAVVDFRPTAPAAAAPDMFALDERGRPIGNASVEGGLASAVPGEVKGLLYA
                     LEHYGSGKLTRAQVIQPAVDWAERGIPVTANLAQIIKDNFAKLRKYEHGLAIYSVDGF
                     PPEAGDTLVNTNLAATLRRIQAEGADAVYAGDIAARIVAEVRKRGGVLTLEDLATYQV
                     KERKPVVGSYRGYSLIAAPPPSGGTHVLQLLNLAEQLGVAALPEQSAEAAHAWAEACK
                     LVFADRTRYSADPDFVRVPVTGLVSKAYAKTLAALVRPDAVLPAPAPPGDPAKYESGS
                     TTSYAVMDRDGNMVAVTKTINMFFGSGVVVPGTGIVMGDDMDDFEARPGSANSVQPGK
                     RMLSSMSPTLVLDPQGRPFMALGSPGATRIIPTLAQVISNVVDRGMPIQAAINAPRLY
                     QGRTGGVSIEGRYSINAYNGLVRLGHAVTVSPDYDPAYGGVHAVLLDREGKVLHGGAD
                     PRRDGQAAGY"
     gene            8921..9523
                     /locus_tag="PLT35_03405"
     CDS             8921..9523
                     /locus_tag="PLT35_03405"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOG28302.1"
                     /translation="MTSTTPIAAAASAIAAAAALPAAFMLAAASAAAAQTFALAAPIA
                     EKPAFVTTVGQSADIEMVKVLLERAAVPHTANAQAKAADLAASGAKTLIVVLGGSTKG
                     LGAAGISVDAELDRARALLAEAKRLKLTVIGVHVGGEARRGQLSDRFIQAAVPACQYV
                     VAVAEGDKDGFISRLCGTAIPIDKVERTSRAVEPLKRAFR"
BASE COUNT         1372 a         3478 c         3428 g         1410 t
ORIGIN      
        1 cttccggtgc acgacgaaca tcggcacgcg ggtgccgtcc ctgctggtga agaagacctg
       61 cttcgtctcg aacgccgacg gatcgaagcc ggggatctcg ggcgcgcgga agaccgtgct
      121 ggccttcgtc gcgatgtcgt agcggtagat cgtcggcggc acgttgaagc tggcgaaggt
      181 gtagaacacg aatgcgtcgt cgcgctcgcc gccgaacccg ccggccgtgc cgagcccggg
      241 cagatcgatc acgtgctcga gcgcgccgtc gagcgcgtgc acctcggcca cggtggccac
      301 gtccttcagg taggtcgcaa acagcttccc gccggcggtg gacgcgccct gcagcggctc
      361 cgggcgctcc gccagcaccg tcacccagtt cggttcctcc ggtcttgtcg ggtcgatcag
      421 cacaactcgg ccgttcggcg cgccgcggtt cgtccgcacc agcagcttgt cgccgacgtt
      481 gtcgatcacg gagaacgtgt cgtcgccgat cgtcccgatg agcggccgga acgccttgtc
      541 cttcctggcc aaatcgcgca cgaacagcgc gttgccgtcc ttgcccttgc cgcggtccga
      601 cacgtcgagg accgcgaagc gctcgtcctc ggtcgtgtcc acccggtgga accgctgcgg
      661 gttcgccgcg tcctcgaaca ccagttcgtc ggccgactgc ggcgtgccga ccttgtggta
      721 gtagacctgg tggttctcgt tcgccgacga gagctcgcgt cccttcgccg gcgccgggta
      781 gcggctgtag tagaagccgt cgccgcgcca cgaggcgccc gacactttca cccactcgat
      841 cctgtcgccg agcatcgtcc tggacgcgac gtccatgacg ccgtattcct gccagtccga
      901 gcccgcgcgc gacaccccga ccaccgcgta cttgccgtcc ttcgacagcg cgaacgccgt
      961 gaggcgggac gtgccgtcgg cggaccacgt gttcggatcg atcagcacct ccggcacgcc
     1021 gtccaggccc tgctggacgt agaggacgct ctggttctgc agcccgtcgt tcttgctgaa
     1081 gtagaagcga tcccccttgc ggcgcggcgc ggtgtacttc gggtagttgt agagcgcctc
     1141 caggcgcgcc ttgaccgcgg cccggtaagg aatctgctcc aggtagccga aggtcacctt
     1201 gttctgcgcc tcgacccagg ccgccgtctc ggccgagttg tcgtcctcga gccagcggta
     1261 cggatcggcg acggcgacgc cgtgatacgt gtcgacgtga tcgaccgtct tcgtctgcgg
     1321 gtagtcgagc ttcggcgccg cctgacgcga gcaggcgatg ccggcgagcg cgacgagggc
     1381 cgcggcggcg gccgtcgggg aggcgatccg gcgaaacacg ctcttcatcc tgggactcct
     1441 gggaatggcg gaccgcgagc ctcagatcga accacagtcg gccagacgga gtccagcggc
     1501 cggagtcggc caggtcgcgg cgcagcattc actcgtgtca ggcgctggcc gcgccgcgca
     1561 cacgcatgcg ggtgtcgcgc gcccggacgg cgcgggtgct gccggcggcg ttcgttgaag
     1621 aagcgcgcgc ggcacaggcg ctggcgccga cgaccggcgc cgtcgaccca gctccgggcg
     1681 ggccggcccg gcggcgacgc gcggacgcct cgccggcgtc gggacccaac tccgtcagtg
     1741 gaccgtgtag ggctcgctca gggcgaaggg cgcgacggtc gcgtcgaagc gctcgccgcc
     1801 ctccgtcacc atccgatagc tgcctttcat catgccgaac ggcgttccca gcgggcagcc
     1861 cgatgtgtag gtgtgcgatt cccccgcccg caggacgggc tgctcgccga caacgcccgg
     1921 gccgtgcact tcctcgaccc ggttgtcgcc gtcggtgatg atccagtgcc gcgccagcag
     1981 ctggacggtg acgtcgctct cgttcgtgat cgtgatcgtg taggggaaga accactgcct
     2041 gcgcgcgggg ttcgagcgct ccggcgcgta gccggactcg gcgtgaacgc gaatgccgcg
     2101 ggtgaccgcc tcggatgtgg gcatgaggcc atcatggtcg aatcgccgcc gtgcgggcaa
     2161 gcggctcggc acgaccggga gacgggcgga cgggaacagg aagcagaagg cgggaccctg
     2221 gaagacggcg ccgccgcgcc cgccgcgacg ccagccgctt gccaaacaca cgcggctgtg
     2281 tgagagtgcc gggggccccg gcgccggggt ccccgtccac cgatggcccc atccaggagt
     2341 cccacgagga gccgacgcgc gttcgcggcc ctctgcctcg ccaccctcgg cgtcgtctac
     2401 ggcgacatcg gcacgagccc gctctacacg atctcggaga tcttcttcgg gccgaaccgg
     2461 attccgtaca ccccgcagaa cgctctcggc gcgacgagcc tcgtgctctg gatcctgacg
     2521 ctgtcggtca cgctcaagta cgtcctgctc gtcctgcgcg cgggctacga gaaccagggc
     2581 ggcaccttcg cgctgctcgc gctcatccag cgctcgacgt ccagcgcgcg caggacgggg
     2641 gctgcgccgc tcctcggggg cctcagcctc gcactggttc tggcggcggc gctgctcttc
     2701 ggcgaaggca tcatcacgcc ggcgatctcg gtcctgtcgg cggtcgaagg cctgaagatc
     2761 gccaccggcg cgttcgatcc cgtcgtcgta ccggccacgg cgctcatcct gctggcgctg
     2821 ttctccattc agagccgggg cacggcgcgc atcggcgcaa ccttcgggcc gatcatcgcg
     2881 ctgtggttcg tcacgatcgc ggggctcggc gtgacgcaca tcctgcggca tcctgccgtg
     2941 ctcggcgcga tcgatccgcg gaacgccgcc gcgttcctgt gggccaacgg cgtccccggg
     3001 tcggtcgcgg tcctgggctc ggtgctgctg gccgtcacgg gctgcgaggc gctcttcgcg
     3061 gacatgggcc atttcggcgc gcgccccatc cgcctcacct ggttctcgct cgtctacccc
     3121 gccctcatgc tgaactacct cggccagggc gcctacgcct acggcggcgg gccggtggaa
     3181 ggcgggcacc tcttctacgc gctcgtgccg cggccgctcc tgctgccgat ggtggcgctg
     3241 gcgacgctcg ccacggtcat cgcctcccag gccctcatct ctggcgcctt ctccctggca
     3301 cggcaggcca tcgccctcgg cctcttcccg cgcctcgaaa ttgtgcacac gagcggggcc
     3361 cacgaaggcc agatctacgt ccccgccatc aaccgtgccc tgctcgtctc gtgcgtcttc
     3421 ctggtcatcg gcttcggatc cagctcgagc ctggccgcgg catacgggtt cgcggtctcg
     3481 ggcgtcatgc tcgtcacgtc gttcgcgatg atgaccatcg cggtgcgcgc ctggggctgg
     3541 ccgccgtgcc tggccgtgcc gctcttcggg ttcttcgccg cgatcgaggc ggtcttcttc
     3601 ttctcggcga gcctgaagat cctccacggt gcctgggtgc cgctcgcggt cggcctgctg
     3661 gccttctcgg tgatgacgac gtggcagtgg ggccgcaggc gggtggcggg cgtgtatgcg
     3721 gagctcgcgc cgcgctgcga aacggtccgg cgcctcatcg agctcaagcg gcacccctcg
     3781 gtgccccagc ttccccgctc ggtcgtcgtc atgtcgtcga ggcccgtgct cgacctcgac
     3841 gaacggatcc ccccggcgct ccacctcttc tgggtgcgcc tcggcgcgct gccgaaacac
     3901 gtcgtcctgc tcaccgtgcg ccagatcccc gtgccgaacg cgcgtgccgt cggggcgccc
     3961 aggaccgagg cggtcacctt cttctcggat ccccgcctcg ggtcggtcgt gggcctgcag
     4021 tccacctacg gctacatgga gacgccggac gtgcggcgcg cgctggtcga ggcgaaggag
     4081 ctgaagaaga tcaaggtgcc gggcgatccg cggaagtggc tcgtgctggt gggccaggag
     4141 aacgtgacgg agacgaaggt cggcgcgctg gtgcgcctcc ggctgtcgtt cttccggacg
     4201 atgctgcgca actcggtgcc ggcgcacctc tacttcggcc tcggctcgga ctcgctcgtc
     4261 acgacggaga ccgtccatct ggccgcggcg gcgttcgacg aggacgatgt cagcgggttc
     4321 gtgccggagg ggccggagcg ggcggtgtga gagcggcgcc ctggcgggcg cgggcgatcc
     4381 ccgccgctca cggcttcgcg gccggccggt acgactgggc gaggatctgc cagagcgtga
     4441 tgaagaacgc ggcaaccagc gggccgacga tgaagcccgg cgcgccgaag aacccgatcc
     4501 cgccgagcgt cgagatcagg atcaccaggt ccggcatctg cgtgtcgcgc ccgaccaggc
     4561 gcggccgcag caggttgtcc acgctgcccg acacgcctgc gcagatgagt gtcagcgtga
     4621 tggcctcgcc gatgcggccc gtcagcgcca gcaggatggc ggcgggcacc cagacgagcg
     4681 cgccgccgat ggccggcacc accgacagca cgaccatcac gacgccccag aacgtcggcg
     4741 ccggcagatc gacgatccag aacgccaggc cgttgatcgt gccctggatg accccgatga
     4801 gcagcgtccc cttcaccgtc gccttcgtca ccgacgcgaa gcgcgcggtc agccgctggc
     4861 tgtcctcgtc gctgaacggc aggtacgaca gcacggcgcg caggtagcgc tcgccgtgga
     4921 cgaagaaaaa gaacatcgcg tagagcagga ttgcggcgtg gaacaggaag acgacggtgc
     4981 cgctcgtcgc ggccgacacc tgcttcacca ggaacgcgcc gaggctggag acgacctgcg
     5041 cgccgcgctc ggctatctgg ggcaggatcg gctcgatccg cgcgctgccc gggatcagcg
     5101 cgagctggct ccgcagcagc tccggatcgt cgagatacgg ctggatcatg cccttggcat
     5161 tggtcgtgag ggagatcgcc tgctgcacga cgatgccggc gatggtgatg agggggccga
     5221 acaccgcgac gacggccaca acgagcgtca ggagcgaggc cagcgtgcgc cggcctcgca
     5281 tgcggcggag cagccagccg tacaaggggt acatcaggcc ggtcagcact gccgccagca
     5341 gcagcgtcat caggaaactg cggatcatgg ccaggaacag gaaggagatc ccggccacga
     5401 tgagcacgag gaagagcttc cggaaccggt cgtcttgcac gaatgcgatc atactcctgc
     5461 ccgcccccct caagctgaac gcctcaggag tcgatattcc gacgagaggt caccatggct
     5521 gaagacacgc cggcgccggt cgttgaagcc cccgagtggg tcgccgcgga aatgccgccc
     5581 ggctaccaga cgaggcgctg ggagatccag cggatgacgg ccgagctcga cgcgatggat
     5641 cgcgtcgcgc gcgtcctctg gagcaccggg gagccgttga aggacgccgt ggccgcggcg
     5701 ttcacggcgc tcaggtgcga agtcgaggcc gccgcggcgc ccggcgggcc cctggtcgcg
     5761 agactgggcg actcgcgccg catgctcgtg gtcgtcgccg gcgcagccgg cccgatcccg
     5821 aagtcggacg agtcgctcgc gcgggcgttc gcggcgatcc agtcggccgg aaccggggac
     5881 cgcgtcgtct tcgtcgctac gagcgacccg gcgtcgccgc ccgccgggcg tcccgacccg
     5941 ctccagcccg acgcgctggc ggtcctcgag cggattggcg tgaccgtggt ggccgcgacg
     6001 acccttttca ggctctggcg gctggccggc gaggatccgc cgaaaggcgg caaggccctc
     6061 gagcggctgc acgcgcaaga gggcggcccg ttctcgctcc cggcgcgctg acccgcccgg
     6121 gcgctcaatc cgactccgtt cccgccgcgc ggcagcccgt cgcggtcccc gcgagcgccc
     6181 gcgtcgcggc aaggccgtcg agcgccgccc aggctgcggc gccagcggcc gcgacgcccg
     6241 cggccgcgcc ggccgcagcg ggaagcctgc cgggcgcgtc ggaacccgcc gtcacgcgca
     6301 ggaacgcgtg gcggggccac cggttgtagc atgtccccgg tccgattccg aggaggcccc
     6361 gccgccatgc accagctcca cttcccgaaa cggttcgagc atacgcaccc gcccgtgcgc
     6421 agcgtcaacg acatcgagac ggagcagttg acgctcggcc agcgggccgc cgatcgcgtg
     6481 gcggccatcg tggggtcgtg gcgcttcatc ggcctgcaga gcctggttct gaccggctgg
     6541 gcctgcgtca acgtcgtggc ctggacgcac cactgggacc cctacccgtt catcctgatg
     6601 aacctgttcc tgtcgctgca ggccgcctac accgcgccga tgctgatgat gagccagaac
     6661 cgcatggcgg cccgcgatcg cctcgaggcg cacaacgact acctgatcaa ccgcaaggcc
     6721 gaggaggagg tgcgcgcggt gctcgaccac ctcagcgcgc aggacgaggc gctgacgaaa
     6781 atcgttgagt cgctcgtcgg gacgctcgag gcgggctccc gcccgccggg gcctggcgcg
     6841 gccgcgccgc gctgacggcc gggggctcgc cggacccggc ggtcctcgga ggtcggagcg
     6901 cacgcagagt tcgccggggc tgccgacgtg cttcacctcg acccgggggc gccggtgaac
     6961 ctcgcgggga tcgggagcca cacggcgcgg tgagcggcgg gcttcgagac gcgccgggcg
     7021 cgccggcggt tcagcggaca cgccgtgccg ccggcgcccg cgatccccgg cgccggcggc
     7081 ggccgggcgg acggaaccag gcggcggaac cggtgcataa tccaacgagc gcggcgctgc
     7141 cgcaggagga tcaccgacat gccacgcccg ggcgcctgcc ggcgccgacc cgcgatcgtg
     7201 tccgtgccgt cggcagttgc cgcggcgctg ctcctcctgc tgcaggcgcg cacgcccgcc
     7261 cagacgccgc tgaacctcta cggccgcggc gcgtcgggcg cgaacggcgt cgtcgcggcc
     7321 gcgaagcctg acgcctcgca ggccggaatc gcgatcctga agatgggcgg caacgctgtc
     7381 gacgcggcgg tggccaccgg gttcgccctc ggcgtcctcg agcccaacgc atccggcatc
     7441 ggcggcggcg ggttcatgct catcaagctc gcctcgatgc aggaggccgc ggtcgtcgac
     7501 ttccggccca ccgcgccggc cgcggctgcg ccggacatgt tcgcgctcga cgagcgcggc
     7561 cgcccgatcg gcaacgcgtc ggtcgagggc ggcctggcat cggccgtgcc gggtgaagtg
     7621 aagggcctgc tgtacgccct cgagcactac ggctcgggga agctgacgcg cgcgcaggtg
     7681 attcagccgg cggtggactg ggccgagcgc ggcattccgg tcacggcgaa cctcgcgcag
     7741 atcatcaagg acaacttcgc caagctccgc aagtacgagc acggcctcgc gatctactcc
     7801 gttgacggct tcccgccgga ggcgggcgac acgcttgtca acacgaacct cgccgcgacg
     7861 ctgcgccgca tccaggcaga gggcgcggac gccgtttacg cgggcgacat cgccgcgcga
     7921 atcgtcgccg aggtgcggaa gcgcggcggg gtgctcacgc tggaggatct cgccacgtac
     7981 caggtgaagg agcgcaagcc tgtcgtcggg tcctaccgcg gctactccct catcgcggcg
     8041 ccgccgcctt ccggcggcac gcacgtcctg cagctgttaa acctcgccga gcagctcggc
     8101 gtggccgcgc tgccagagca gagcgccgag gcggcgcacg cgtgggccga ggcctgcaag
     8161 ctcgtgtttg cggatcggac gaggtactcg gcagaccccg acttcgtgcg tgtgccggtg
     8221 acggggctcg tgtcgaaggc ctacgccaag acgctcgccg cgctcgtcag gcccgatgcg
     8281 gtgctgccgg cgcccgcgcc gccgggcgac cccgccaagt acgagtcggg ttcgacgacg
     8341 tcctatgccg tgatggaccg cgacgggaac atggtggcgg tcacgaagac gatcaacatg
     8401 ttcttcggct ccggcgtcgt ggtgcccggc acgggcatcg tcatgggcga cgacatggac
     8461 gacttcgagg ccaggcccgg atcggcgaac tcggtccagc ccggcaagcg gatgctgtcg
     8521 agcatgagcc cgacgctcgt gctcgacccg cagggccggc cgttcatggc cctcggatcg
     8581 ccgggcgcga cgcgcatcat ccccacgctg gcccaggtca tctcgaacgt cgtcgatcgc
     8641 gggatgccga tccaggccgc catcaacgcg cctcgcctct accagggccg gacgggcggc
     8701 gtgtccatcg aggggcgcta ctcgatcaac gcgtacaacg ggctggttcg cctgggccac
     8761 gcggtgacgg tgagcccgga ttacgacccg gcgtacggcg gcgtgcacgc cgtgctcttg
     8821 gatcgcgagg ggaaggtcct gcacggcggg gccgacccgc ggcgcgacgg gcaggccgcg
     8881 ggatactgag aatggccggt acgaaaccag ggagcgcact gtgacgagca ccacgccgat
     8941 cgccgccgcc gcgtccgcca ttgcggcggc cgccgcgctg ccggccgcct tcatgctcgc
     9001 cgccgcgagc gcggcagccg cccagacctt cgctctcgcg gcgcccatcg ccgagaagcc
     9061 cgccttcgtc accaccgtcg gccagagcgc cgacatcgag atggtgaagg tgctgctcga
     9121 gcgcgccgcc gtgccccaca ccgccaacgc ccaggcgaag gcggccgatc tggccgcgtc
     9181 cggggcgaag acgctgatcg tggtgctcgg cgggagcacc aaggggctgg gcgccgcggg
     9241 catctccgtc gacgccgagc tggaccgggc cagggcgctc ctagccgaag cgaagcggct
     9301 caagctgacg gtgatcggcg tgcacgtggg cggcgaggcc cgccgcggcc agctctcgga
     9361 ccgcttcatc caggccgccg tgcccgcgtg ccagtacgtc gtggccgtcg ccgagggcga
     9421 caaggacggg ttcatcagca ggctgtgcgg gacggccatc ccgatcgaca aggtggagcg
     9481 cacgtccagg gccgtcgagc cgctcaagag ggccttcagg tgacggacgg acgcggcacg
     9541 tcctcccgcg cggcgggtcg ctcatgagcg gccccgacgc cctgctgctc ctcctcacgg
     9601 ccggcagctt cgccgccctc gtgatcgcgg cgaagctgcc cgtgtcgctc agcctggcgg
     9661 cctcgtcggt cgcgctggcg ctggcggt
//
LOCUS       DAOSIF010000057         9416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_6993_length_9416_cov_3.270377, whole genome shotgun sequence.
ACCESSION   DAOSIF010000057 DAOSIF010000000
VERSION     DAOSIF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6993_length_9416_cov_3.270377"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /gene="glgX"
                     /locus_tag="PLT35_03410"
     CDS             complement(<1..1398)
                     /gene="glgX"
                     /locus_tag="PLT35_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOG28303.1"
                     /translation="MRVWPGRPHPLGATWDGLGVNVALFSEHATGVDICLFDTASDAR
                     ETHRIPLSERTDFVWHAYLPDVRPGQLYGFRVHGPYAPEAGHRFNPAKVVLDPYARAI
                     GRRLRWNDAIFDFAPGGQGEGVVRDDRDSAAFAPLAAVIDPAFTWGDDRPPQTPWHRT
                     VIYEVHVKGFTFRHPQVPPLLRGTFLGMASDAAIRHLTDLGVTAVELLPVHLHASDRH
                     LIARGLPNYWGYNSLNYFVPDPRFASARGRMAAVREFKMMVRALHAAGLEVILDVVYN
                     HTAEGDHLGPTLSLRGIDNASYYRLAPGDPSRYQDFTGCGNTLNMRHPRVLQLMMDSL
                     RYWVVDMHVDGFRFDLASALARELFEVDKLGSFFDIMRQDPVLSQVKLIAEPWDLGEG
                     GYQVGNFPAGWTEWNARYRDSVRRFWRGDGGQVSELASRLAGSSDLYAQGGRRPYASI
                     NFVTCHDGFTLRDLVS"
     gene            1519..2502
                     /locus_tag="PLT35_03415"
     CDS             1519..2502
                     /locus_tag="PLT35_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOG28304.1"
                     /translation="MYLRVMKVGCFGGGTGLSSVLGGLKRNPWLDLNAVVTMFDSGGS
                     SGQLRDELGVLPPGDVLKCALALARNEREARRVLLSRVPVFEDSRLGGHTGGNLLLSM
                     MEQYSGSFLAGVEGLRALLGCRGRVWPVSIEQASLCARYTDGSDARGEVSVDARLGSG
                     HAIERIWLEPPVRIHPEVAAAIAGLEAVIVGPGSFFTSLMPVFAVEGVAAAVAQVRGP
                     LILIANLLTEGRGMDGFTAADAVRRIGEAAGRPVTAVVFNESRPSAEVLARYHAEDKR
                     PLELGTLPDSCMLVSGRFWKSDIARHDRSRLAHALWAVLAANLERRSRPRA"
     gene            2529..3785
                     /locus_tag="PLT35_03420"
     CDS             2529..3785
                     /locus_tag="PLT35_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOG28305.1"
                     /translation="MADSEFGILGILIATLGGAAIGVERERSGHASGPTARFAGVRTF
                     TLLGGLAGVCGWLWASGSAALSAVILAGAAGLVVAAYFAAAPRDPDGTTEVAALVTIA
                     AGVVAGVGHLALSSAVIAVTALLLIEKSRVHATVARLDETAVRAGIRFAVMAVVLMPL
                     LPAGPYGPLGGVKPRELWLFVLFFSGISFAGYLARLFVGARHGYLIAGLLGGIVASTG
                     VTLAFSRASRREDQSVGRPLAYGVVAACTMSFVRVMVATAVLFLPLAAALVPLLAAPF
                     LVGVAVVLLGLRRPWQERVEVQTLRNPLEFRAALQMAVLFQAVLFLVAGVRAVWGDLG
                     LVVSGAVLGLANVDALMISMAKTAAVHGSAATPAVAIAVGILSNTLFKLALAAGLGRG
                     SFRIVAGAGLAAMAAASAIALSWGRL"
     gene            3892..4656
                     /locus_tag="PLT35_03425"
     CDS             3892..4656
                     /locus_tag="PLT35_03425"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOG28306.1"
                     /translation="MRVDLNCDMGEGFGAYAPGSDRDLIGCVTSANIACGFHAGDPGW
                     MRTTVALAEAHGVAVGAHPGLPDLRGFGRRTLDATPAEIRDDLVYQIGALTAFTAGKR
                     LQHVKPHGALYAMAVRRADVAGAVADAIQSVDQRLILVVLAGSPWERWSRERGLRVAR
                     EAFADRAVDARGGLVPRGRPGSVLTDAQEVVERSLRLVIEHRVAAITGEDVEVHADTI
                     GLHGDTPGAPALAAALKNALNRAGVDVAPLGRWLAG"
     gene            4660..5397
                     /gene="pxpB"
                     /locus_tag="PLT35_03430"
     CDS             4660..5397
                     /gene="pxpB"
                     /locus_tag="PLT35_03430"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HOG28307.1"
                     /translation="MPALPRILDAGDSAIVVEYGRGIDAAANARVRRLDAALRQAPRG
                     GILETVPAYCSLLVHYDPLAITREALERSVREADGAAGEGAAESARTITIPVAYGGAF
                     GPDLADVAAFAGLTEDAVAAMHAAGRYRVFMLGFMPGFPYLGGLPPRLAAPRLATPRT
                     LVPGGSVGIAGEQTGVYPADGPGGWRIVGRTPIPLFDARQTPAALIDAGDRVRFVAIA
                     SGEFDAIARRVRAGAWRRAEAVGNGAA"
     gene            5394..6674
                     /locus_tag="PLT35_03435"
     CDS             5394..6674
                     /locus_tag="PLT35_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HOG28308.1"
                     /translation="MSGVSVLAGGMLTTVQDLGRTGARRWGVPLSGALDRWSLRAANR
                     LAGNADAAAALEMTFVGPVLRFEQPSVAALSGADLGAMLDGHPVAPWRSFSVAAGAVL
                     TFTGARDGLRGYLAVAGGFDVPAVLGSRSTFLRAGLGGLDGRPLRAGDCLPLGSAAQP
                     DGLVRRLPRRAIPTYGHAPAIRVVLGPQDDAFTDEGIGAFLGGTYTLALQSDRTGCRF
                     LGPPIAHRGPADIVSDGTVFGSVQVAGDGLPIVLMADGGTTGGYPKIATVATADLPRL
                     AQTAPGDRVRFSRVGVEEAHALLRAAFEALDAIGPAAPGGAADEEVYEEDSAGSLVAD
                     AYVGLADAVDGAHSIPECARRNAVCAAMPGLVVSVAVRSGECVAPGQTLLVLDAMKME
                     NPVRAPRAGRVGRIHVVPGVMVEGGAPLVDVDEA"
     gene            complement(6796..7734)
                     /locus_tag="PLT35_03440"
     CDS             complement(6796..7734)
                     /locus_tag="PLT35_03440"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOG28309.1"
                     /translation="MRRFSAAAARDATLLLAWGLFKKLVIADNVGVIANKVFALREPE
                     FHVLWAGVFAFAVQIYADFSSYADIARAVAKWLGIDLVRNFDHPYLAASPAEFWQRWH
                     ISLSTWFRDYVFLPVAYRISGRLTAEKRFGLDAATRAYAGGIMVTMLAAGLWHGAGWN
                     YVLWGGYHGVLLALARLLAPRRGRRRRARRWLRPARAAGMFALTAIGWLFFRETDGSM
                     ILRDLALSPFQASALGRQTGAYLFFLAGLYSIPLWIDSLWSKLGGPSLTAAMVREDAR
                     LDGRLVAAQALLAGTLVAGALTLHATTSLDFIYFRF"
     gene            7768..7893
                     /locus_tag="PLT35_03445"
     CDS             7768..7893
                     /locus_tag="PLT35_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28310.1"
                     /translation="MIGPASRLGKKDTNAATSTRLRRARIRPKYTSMGYVTAWNV"
     gene            complement(8264..9313)
                     /locus_tag="PLT35_03450"
     CDS             complement(8264..9313)
                     /locus_tag="PLT35_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28311.1"
                     /translation="MPSSGLPRRQRLSLAAVILGAAIVPFGLRAVNAVTRPPGAEPSY
                     IPSVESPRVRGPFDREAVEALREAANDVILIGDSMAGTRIHPGHLSRLMGGRGAAALF
                     HPGSGPAYWYLTFKNLVVNAGLHPRTAVFFFRDENLTDTLFRVYPSALDRVARQREPV
                     LDELLAARANGRYFRVHRILTRLYGHDQARAWLEPLIVRAPVAWSATRRARKRLLERI
                     NNEVFTLDALRPMAAADMASASGEALDFRRNLPTSVLPEILRLAHSSGIRVAFVRVQR
                     RPGPDGPPPQSAALRAYVGALRAYLEENGALFHDDWGDPDQPLSIYEDGDHIAREYRL
                     HCTELFFRKNPEVFR"
BASE COUNT         1252 a         3316 c         3527 g         1321 t
ORIGIN      
        1 gctcaccaga tcgcgcagcg tgaagccgtc gtggcaggtg acgaagttga tgctcgcgta
       61 cggccggcgg ccgccttggg cgtagaggtc gctgctgccg gcgaggcggc tcgccagttc
      121 cgacacctgg ccgccgtcgc cgcgccagaa ccgccgcacc gagtcgcggt agcgggcgtt
      181 ccattcggtc catccggcag ggaagttccc gacctggtac cccccctcgc cgagatccca
      241 gggctcggcg atcagcttga cctgcgacag caccgggtcc tgccgcatga tgtcgaaaaa
      301 cgaacccagc ttgtcgacct cgaacagttc gcgcgcgagg gcgctcgcga ggtcgaagcg
      361 gaagccgtcc acgtgcatgt ccaccaccca gtaccggagg ctgtccatca tgagctggag
      421 caccctcgga tggcgcatgt tcagcgtgtt gccgcagccg gtgaagtcct ggtaccggct
      481 cgggtcgccg ggcgccagcc ggtagtacga cgcgttgtcg atgccgcgca gggacagcgt
      541 cgggcccagg tggtccccct cggccgtgtg gttgtagaca acgtccagga tgacttcgag
      601 gccggccgcg tggagcgcgc ggaccatcat cttgaactcg cggacggccg ccatgcgccc
      661 gcgagcgctc gcgaaccgcg ggtcggggac gaagtagttc agcgagttgt agccccagta
      721 gttcggcagg ccccgcgcga tgaggtggcg atcgctggcg tggaggtgca cgggcaggag
      781 ttcgaccgcg gtgacgccga ggtcggtcaa gtggcggatc gcggcgtccg aggccatgcc
      841 cagaaacgtg ccgcgcagca gcggcggaac ctgcgggtgc ctgaaggtga accccttgac
      901 gtgcacctcg tagatgaccg tccggtgcca cggcgtctgg ggaggccggt cgtcgcccca
      961 ggtgaacgcc ggatcgatga cggcggccag cggcgcgaag gcggcgctgt cgcggtcgtc
     1021 gcggacgacg ccctcgccct ggccaccggg cgcgaagtcg aagatcgcgt cgttccagcg
     1081 cagacgccgt ccgatcgcgc gcgcgtaggg gtcgagcacc actttggccg gattgaaccg
     1141 gtggcccgcc tccggcgcgt acggcccgtg gacgcgaaac ccgtagagct gaccgggccg
     1201 cacgtcgggc agatacgcgt gccacacgaa atcggtgcgc tcagaaaggg gaatccggtg
     1261 cgtttcccgc gcgtcggacg ccgtgtcaaa caagcagatg tccacgccgg ttgcgtgttc
     1321 ggagaagagc gcgacgttga cgcccaggcc gtcccacgtc gcgcccagcg gatgaggtcg
     1381 tccgggccag acgcgcatgc ccgcattgta cgcggtcggc ggctctggcg ccgaacgatc
     1441 cgccccggtc gaaccggcgc ggaggcgggt gcggcggcgg tccgagccgc cgggcggcgg
     1501 cgctgataga atccccgtat gtacctgcgg gtcatgaagg tcggttgctt cgggggcggc
     1561 acggggctct ccagcgtcct cggcggcctc aagcggaacc cgtggctcga tctcaacgcc
     1621 gtcgtgacga tgttcgacag cggcggcagt tcggggcagc tgcgcgacga gttgggcgtt
     1681 ctgccgccag gcgacgtgct gaagtgcgcg ctggcgctgg cgcgcaacga gcgggaggcg
     1741 cggcgcgtcc tgctgtcgcg cgtgccggtc ttcgaggact cgcgcctggg cggccacacg
     1801 ggcggcaacc tcctgctgtc gatgatggag cagtacagcg ggagcttcct ggccggcgtc
     1861 gaggggctcc gcgccctcct gggctgccgg gggcgcgtct ggccggtcag catcgagcag
     1921 gcgagcctgt gcgcgcggta caccgatggg tcggacgcca ggggcgaggt ctccgtggac
     1981 gcgcgtctgg gctccggcca cgccatcgaa cgcatctggc tcgagccgcc ggttcggatc
     2041 catcccgagg tggccgccgc gatcgccggc ctcgaagccg tcattgtcgg tccggggagc
     2101 ttcttcacga gcctgatgcc ggtcttcgcc gtggagggcg tggccgcagc ggttgcccag
     2161 gtgcgcgggc cgctcatcct catcgcgaac ctcctgaccg aggggcgcgg catggacggg
     2221 ttcacggccg ccgatgcggt gcggcgcatc ggcgaggccg ccggccggcc ggtcaccgcc
     2281 gtggtcttca acgaatcccg gccgtcagcc gaagtgctgg cgcgctacca cgcggaggac
     2341 aagcgcccgc tcgagctggg cacgctgccc gactcgtgca tgctcgtcag cggccggttc
     2401 tggaagtcgg acatcgcgcg ccacgaccga tcgcggctgg cgcacgcgct ctgggcggtg
     2461 ctggcggcga acctcgaacg gcggtcgcgg ccccgggcct gacggggccg gccgcccgcg
     2521 gacattccat ggctgactcg gagttcggca tcctgggcat cctgatcgcc accctgggcg
     2581 gcgccgcgat cggcgtcgag cgggagcggt cgggccacgc cagcggaccg acggcgcgct
     2641 ttgccggcgt gcggaccttc acgctgctcg gcggccttgc cggggtgtgc ggctggctct
     2701 gggcttccgg ctcggcggcc ctctcggccg tcatcctggc cggggcggcg ggcctcgtcg
     2761 tcgcggcgta cttcgccgcc gccccgcgcg atcccgacgg caccacggaa gtcgccgcgc
     2821 tcgtcaccat cgcggccggc gtcgtggcgg gcgtggggca cctggccctc tcgagcgcgg
     2881 taatcgccgt cacggccctg ctgctcatcg agaagtcccg cgtgcacgcc accgtcgcga
     2941 ggctcgacga gacggccgtc cgggccggca tccgcttcgc cgtgatggcg gtcgtgctga
     3001 tgccgctgct gccggccggg ccgtacgggc ctctcggcgg cgtgaagccg cgcgaactct
     3061 ggctgttcgt cctcttcttc tcgggcatca gcttcgccgg ttacctggcg cgcctgttcg
     3121 tcggcgccag gcacggctac ctgatcgccg gcctcctcgg cgggatcgtc gcctcgacgg
     3181 gcgtgacgct ggcgttctcg cgcgcgagcc gccgcgagga ccagagcgtc ggccggcccc
     3241 tcgcatacgg ggtggttgcc gcctgcacga tgtcgttcgt gcgcgtgatg gtggcgaccg
     3301 ccgtgctgtt cctgccgctg gcggccgcgc tggtgccgct cctggcggcg ccgttcctgg
     3361 tgggtgtcgc cgtcgtactc ctggggctcc ggcggccctg gcaggagcgc gtcgaggtcc
     3421 agacgctccg caacccgctg gagttccgcg ccgccctcca gatggcggtg ctcttccagg
     3481 cggtgctgtt tctcgtggcc ggcgtgcgcg cggtgtgggg cgacctcggg ctcgtcgtgt
     3541 cgggcgccgt gctcggcctc gccaacgtcg acgcgctgat gatctcgatg gcgaagaccg
     3601 cggcggtcca cgggtccgcg gcgacccccg ccgtggcgat tgcggtgggc atcctctcga
     3661 acacgctctt caagctcgcg ctggcagccg gacttggacg cggctcgttc cggatcgtgg
     3721 ccggcgcggg gctggcggcg atggcggcgg cgtcggcgat cgccctgagc tggggccgcc
     3781 tctagcggcg cgcttcccgc cgtccggttc gcggcggaag tcgcgccggt tccttgacat
     3841 cgtgcctgtg cgccactagc ctgtggcccg atgccggttc caggcccgcc cgtgcgcgtc
     3901 gacttgaact gcgacatggg cgaaggcttc ggcgcgtacg cccccggcag cgatcgcgat
     3961 ctgatcggct gcgtcacctc ggcgaacatc gcgtgcgggt tccacgcggg ggatcccggg
     4021 tggatgcgga cgaccgtggc gctggccgag gcgcacggcg tggccgtcgg cgctcacccg
     4081 gggcttcccg acctgcgggg cttcggccgg aggacgctcg acgcgacccc cgccgagatc
     4141 cgtgacgacc tagtctacca gatcggcgcg ctgacggcct tcacggcggg caagcggctg
     4201 cagcacgtga aaccgcacgg cgcgctgtac gccatggccg tccgccgcgc ggacgtggcg
     4261 ggggctgtcg ccgatgcgat tcagtccgtg gatcagaggc tcatcctcgt cgtgctcgcc
     4321 ggctctccgt gggagcggtg gtccagggaa cggggcctgc gcgtcgcgcg cgaggctttc
     4381 gcggaccgcg cggtcgacgc ccggggcgga ctcgtgcccc gcggcaggcc cggctccgtc
     4441 ctgaccgacg cgcaggaggt ggtcgagcga agcctgcggc tcgtcatcga gcatcgggtg
     4501 gcggccatca ccggtgagga cgtcgaggtg cacgccgaca ccatcggttt gcacggggac
     4561 acgcccggcg cgccggccct cgcggcggcc ctgaagaacg ccttgaaccg tgccggcgtg
     4621 gacgtggcgc cgctcggccg gtggctggcg ggctgacccg tgcccgccct gccgcgcatc
     4681 ctcgacgccg gagacagcgc catcgtcgtc gagtacgggc gcggcatcga cgcggccgcc
     4741 aacgcgcgcg tgcggcggct cgacgcggcg ctccgccagg cgccgcgcgg cggcatcctg
     4801 gagacggtgc cggcctactg ttcgctgctg gtgcactacg acccgctggc gatcacgcgc
     4861 gaggcgctcg agcgctcggt gcgagaggct gacggcgcgg cgggcgaggg cgccgccgag
     4921 agcgcgcgga cgatcacgat cccggtggcg tacggcggcg ccttcgggcc cgacctcgcc
     4981 gacgtcgccg cattcgctgg cctgaccgag gacgcggtcg ccgccatgca cgccgccggc
     5041 aggtaccgcg tcttcatgct gggcttcatg cccgggttcc cgtatctcgg cggcctgccg
     5101 ccgcgcctcg ccgcgccgcg cctcgccacg ccgcgcacgc tcgtgcccgg cggctcggtc
     5161 ggcatcgccg gcgagcagac gggcgtctat ccggccgacg gccccggcgg gtggcgcatc
     5221 gtcggccgga cgcccattcc tttgttcgac gcgcggcaga cgccggctgc cctcatcgac
     5281 gcgggcgatc gagtccgctt cgtggcgatc gcgagcggcg agttcgacgc gatcgcacgc
     5341 cgggtccgcg ccggcgcatg gcggcgcgcg gaggccgtcg ggaacggggc ggcatgagcg
     5401 gcgtctccgt gctcgccggc ggcatgctca cgaccgtcca ggacctcggg cggacggggg
     5461 cgcgtcgctg gggcgtgccg ctctcgggcg ctctggaccg gtggtcgctt cgggccgcga
     5521 atcgtctcgc gggcaacgcc gacgccgcgg ccgccctgga gatgaccttc gtcgggccgg
     5581 tcctgcgctt cgagcagccc tcagtggcgg cgctctccgg cgcggacctg ggcgccatgt
     5641 tggatggcca tccggtcgcg ccctggcgct ccttcagcgt tgcggccggg gccgtgctca
     5701 ccttcaccgg agcccgcgac ggcctgcgcg ggtacctcgc cgtggccggg ggcttcgatg
     5761 tccccgcggt gctcgggagc cgttcgacgt tcctcagggc gggcctcgga ggcctcgacg
     5821 gccgtccgct gcgcgctggc gactgcctgc cactcgggtc ggccgcccag ccggacggcc
     5881 tcgtgcggag gctcccgcgt cgggcgatcc cgacctacgg ccacgcgccc gcgatccgcg
     5941 tcgtcctggg gcctcaggac gatgcgttca ccgatgaggg catcggcgct ttcctcggcg
     6001 ggacctacac gctggcgctg cagtcggatc gcaccggctg ccggtttctg ggccctccca
     6061 tcgcgcatcg ggggccggcc gacatcgtgt ccgacgggac cgtgttcgga tcggttcagg
     6121 tcgctggaga cggcctgccg attgtgttga tggccgacgg cggcacaacc ggcgggtatc
     6181 cgaagatcgc caccgtcgcc acggccgacc tgccgaggct ggcgcagacg gcccccggcg
     6241 accgggtgcg gttctcgcgc gtcggcgttg aagaggcgca cgcgctgctg cgagcggcgt
     6301 tcgaggcgct ggatgcgatc ggccccgccg cgccaggcgg cgctgccgac gaggaggtct
     6361 acgaggagga cagcgccggt tcgctcgttg ccgatgcgta cgtcggcctg gcggacgccg
     6421 ttgacggcgc tcacagcatc ccggagtgcg cgcgccgcaa cgcggtgtgc gcggccatgc
     6481 ccggcctggt cgtgtcggtg gccgtgcggt ctggcgagtg cgtcgccccg gggcagacgc
     6541 tcctggtcct cgacgcgatg aagatggaga accccgtccg cgcgccgcgc gcggggcgcg
     6601 tcggccgcat tcacgtggtg ccaggcgtca tggtcgaagg cggtgcgccg ctcgtggacg
     6661 tcgacgaggc gtgaggagcg ccgtcctccc gctgtcggcc gcggccctcg tcctcgcggc
     6721 ggccttgtcg gcccggctcc tcccaccgac gcgcgcgcgc ccgatcggct gcggcgcccc
     6781 cgggcgccgc tccgatcaga acctgaagta gatgaagtcg agcgaggtcg tggcgtgcag
     6841 cgtgagggcg ccggccacca gcgtgccggc gagcagcgcc tgcgccgcga cgaggcggcc
     6901 gtcgagccgc gcgtcctcgc gcaccatcgc cgccgtcagg ctcggtccgc ccagcttcga
     6961 ccagaggctg tcgatccaca ggggaatcga gtagagcccg gccaggaaga acaggtaggc
     7021 cccggtctgc cggccgagcg cgctggcctg gaacggggac agcgccagat cgcgcaggat
     7081 catcgagccg tcggtctcac ggaagaacag ccacccgatg gccgtcagcg cgaacatccc
     7141 ggcggcgcgg gcgggccgga gccagcgccg ggcgcggcgg cggcgcccgc ggcgcggcgc
     7201 cagcagcctc gcgagagcga gcagcacgcc gtggtagccg ccccagagga cgtagttcca
     7261 gccggcgccg tgccagagcc ccgctgcgag catcgtgacc atgatgccgc ccgcgtaggc
     7321 ccgcgtcgcc gcgtcgaggc cgaagcgctt ctcggccgtg agccggccgg agatccggta
     7381 cgcgacgggc aggaacacgt agtcgcggaa ccaggtggag agcgagatgt gccagcgctg
     7441 ccagaactcc gccggggacg ccgccagata cggatgatcg aagttcctga cgaggtcgat
     7501 gcccagccac ttggccaccg cgcgcgcgat gtcggcgtag gacgagaagt ccgcgtagat
     7561 ctggacggcg aaggcgaaca cgccggccca caggacgtgg aactccggct cccgcagcgc
     7621 gaagaccttg ttcgcgatga cgccgacgtt gtcggcgatg acgagcttct tgaacagccc
     7681 ccaggcgagc agcagggtgg cgtctcgggc cgcggccgcg gaaaaccggc gcacgccctc
     7741 gatctgggca gcaaagtcga cgcccgcatg atcggaccgg ccagcaggct ggggaagaag
     7801 gacacgaacg cggccacgtc caccaggctg cgccgcgcgc gcatccgccc gaagtacacg
     7861 tcgatgggat atgtgacggc ctggaacgtg tagaaggaga tcccggccgg caggacgagg
     7921 ctcaaggcgg ggcgcggaac gcggagaccc gcgagcgaga gcgccgccgc gacgttctcg
     7981 gcgaagaacc cgaagtactt gaaggccccc agcacgccga aggtgacgaa gacgccgaac
     8041 cacaggaacg tcttccggcg cgacggccag acctccatgc cgagcccgga ggcataggtg
     8101 acaaccgcca ctgtcgccac cagcgacagg agccacgggt ggatccagcc gtagaagacg
     8161 gcgctcgcgg cgagcagcag caggttctgc ccgcggtgcg gcagcgccca gtagagcggc
     8221 accacgagga cgaagaagat cgcgaagtcg aggctgtgga agatcaccgg aagacctccg
     8281 ggttcttcct gaagaacagt tccgtgcagt gcagccggta ctcccgcgcg atgtggtcgc
     8341 cgtcctcgta gatcgacagc ggctggtccg ggtcgcccca gtcgtcgtgg aacagcgcgc
     8401 cgttctcctc gagatacgcc cgcagcgcgc ccacgtacgc ccgcagcgcc gccgactgag
     8461 ggggagggcc gtccgggccc ggccgccgct gcacgcggac gaacgcgacg cggatccccg
     8521 acgagtgcgc cagccggagg atctcaggga ggaccgacgt cggcaggttc cgccggaagt
     8581 cgagcgcctc tccgctcgcc gatgccatgt ccgccgccgc catcggccgg agcgcgtcga
     8641 gcgtgaacac ctcgttgttg atccgttcga gcagccgctt ccgggcccgg cgcgtggccg
     8701 accaggcgac aggcgccctg acgatgagcg gctcgagcca cgcccgggcc tggtcgtggc
     8761 cgtacaggcg cgtcaggatg cggtggaccc ggaagtagcg cccgttcgcg cgcgccgcca
     8821 gcagttcgtc gaggacgggc tcccgctggc gcgcgacccg atcgagcgcg gacggataga
     8881 cccggaacag cgtgtcggtc aggttctcgt cgcggaagaa gaagacggcc gttcgcgggt
     8941 gcaggcccgc gttcaccacg aggttcttga aggtgagata ccagtaggcc gggcccgagc
     9001 cgggatggaa cagggccgcg gcgccgcggc cgcccatcag gcgcgacagg tggccgggat
     9061 ggatgcgcgt cccggccatc gagtccccga tgaggatgac gtcgtttgcg gcctcccgca
     9121 aggcctcgac cgcctcacgg tcgaacggcc cgcggaccct cggggactcc accgacggga
     9181 tgtagctggg ctcggccccg ggcggccgcg tcaccgcgtt caccgccctg agcccgaagg
     9241 gaacgatggc ggcgccgagg atcacggcgg ccagcgagag gcgctggcgg cgcggcaagc
     9301 ctgacgacgg catccgacga ggctccgggg gacggcctag cgggccccgg ccgccgccgc
     9361 cgggaacagc ttctcgagca ccttaagctc cggaggcggc gtcagcaggc tgacgg
//
LOCUS       DAOSIF010000058         9261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7108_length_9261_cov_3.712470, whole genome shotgun sequence.
ACCESSION   DAOSIF010000058 DAOSIF010000000
VERSION     DAOSIF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7108_length_9261_cov_3.712470"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLT35_03455"
     CDS             complement(<1..745)
                     /locus_tag="PLT35_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOG28312.1"
                     /translation="MTSPLDRLADLRRRAELGGGEARLKRQREAGKLTARERMEALFD
                     PGTFEELDKLVTHRCLDFGMQEQLVPGDGVVSGHGRVNGRPVFAFAQDFTVFGGSLSE
                     TNAAKICKVMDLAVRMGAPIVGLNDSGGARIQEGVMSLAGYADIFLRNTLASGVVPQI
                     SAIMGPCAGGAVYSPAITDFNIMVKQTSYMFVTGPEVIKTVTHEDVTKEELGGAMTHN
                     ERSGVAHFAAEDDRECLALIRELLGFLPSN"
     gene            complement(742..1395)
                     /gene="fsa"
                     /locus_tag="PLT35_03460"
     CDS             complement(742..1395)
                     /gene="fsa"
                     /locus_tag="PLT35_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOG28313.1"
                     /translation="MKLFLDTGNIRDIEAYCAIGIIDGVTTNPSLMAREEGDPRAITK
                     RICEIVRGPVSAEVLATDAEGMIREGRDLAAIDPHVVIKVPFGKDGVQACKALREDGI
                     GVNVTLVFTPAQALLAAKAGASFVSPFVGRLDDVATDGMELVAQIVEIYENYGYQTEI
                     IVASVRHPMHVVQAARLGAHICTCPAAVIEALFKHPLTDVGIERFLRDWAAAKGARP"
     gene            complement(1689..2951)
                     /locus_tag="PLT35_03465"
     CDS             complement(1689..2951)
                     /locus_tag="PLT35_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOG28314.1"
                     /translation="MRPSRVALVVCVAAAGLAWPGCGKKGPPLAPFVRAPGRAMDLQA
                     RRSGDLVTLTFTVPSANIDDTRPADLAALEVYAYTAMAQNDVRDTARMTLVAKIPVRK
                     PAALAPGAASRPAEPAPRGDSAADQGALVTITETLTADMQAPLPPDGRRKTVRAPAEP
                     RAWLDPPPPPPLGGPAAAAIPNRYYLVYGVSRSGNRGGASPRPAVPLVPPPPAPEQPA
                     LSVTEEGVVVRWAVPPGARLPYQAPASDDALAASTFGMESAPALSYVISLGSVRLTDT
                     PVKRLTWTDANAPYGAERCYDVRAVLVRGAASVESEPSLAACVTPVDTFPPPAPSGLA
                     AVAGEGAVSLIWTAVSAPDLAGYIVLRGEAGSAALEPLFTAPVMETTYRDASAKPGVR
                     YVYAVAAVDTAAPGNRSALSNTVEETAR"
     gene            complement(2948..4318)
                     /gene="hslU"
                     /locus_tag="PLT35_03470"
     CDS             complement(2948..4318)
                     /gene="hslU"
                     /locus_tag="PLT35_03470"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOG28315.1"
                     /translation="MPLLPPASPDALASAPTPRQIVSELDKYVVGQHRAKRAVAIALR
                     NRLRRQQLPAEMAEEVAPKNILMIGPTGVGKTEIARRLARLARAPFLKVEASRFTEVG
                     YVGRDVESMIRDLVEIAVGMVREERVREVRHKAEQLAEERLLDLLLPPRPPSAEGHPA
                     VDPAAQTRERLRAQLREGKLDHRTVEVDVRESALPMMQVVGGSSMEELGLNLRDMLPG
                     MFQGRRRSSTMKVPEARAHLVEEEQQKLVDVESVARTAIERVEQGGILFVDEIDKIAG
                     REGGHGPDVSREGVQRDILPIVEGTTVNTKHGMVRTDHILFIAAGAFHVSRPSDLIPE
                     LQGRFPIRVELEALGRDDFVRILREPRNALVTQYTALLATDGVELHFTDDAIDRLAEL
                     AALVNERTENIGARRLHTVMEKLLDEVSFEGPDLAGRSITIDAAYVDRMLADAVKNED
                     LSRYIL"
     gene            complement(4339..4878)
                     /gene="hslV"
                     /locus_tag="PLT35_03475"
     CDS             complement(4339..4878)
                     /gene="hslV"
                     /locus_tag="PLT35_03475"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOG28316.1"
                     /translation="MTHSTTILAVRHHGRVVMACDGQVTLGNAIVKQGARKIRRLYAD
                     KVLAGFAGSAADSFALFSRFEGKLEQYRGNLERSAVELARDWRTDRALRRLEAMLIVA
                     DERATFLLSGTGDLIEPDDGIVGIGSGGLYAVAAARALARHTQIEARVIAEIAMGIAA
                     GICIYTNANLSIEELTPTS"
     gene            5032..5970
                     /locus_tag="PLT35_03480"
     CDS             5032..5970
                     /locus_tag="PLT35_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28317.1"
                     /translation="MRRLAFSGVVFAALMAALLVAGPSGVFAQSPRGRGAGQGGSAAQ
                     PRGGGAAVSPGGASSGGGSRQPSSAAPRSGRAGGGNAGSSAGREARPRDGGGSWRSPA
                     DGQAAGRARDAGGADVVGVPWYSRPRGGNPSTGTAIPRADAPAPQGGGGYYVFNQNYY
                     NGYYGYRYNPFNWYGNVWGYGAFGLGYFYYDPFWWSYEDYRYRNYGGYSSLPYGYGYG
                     YGPAGYGDGYATGTREPMGGVRLKVKPNTASVYVDGYYAGRVDDFDNMFQKLQLPLGL
                     HRIEIAAPGYKPLVFELNIRSWDTITYEAWLEPIAR"
     gene            5946..6443
                     /locus_tag="PLT35_03485"
     CDS             5946..6443
                     /locus_tag="PLT35_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28318.1"
                     /translation="MARTDRQVAAAVERSSRATGEPRVAMPGASLSPSVLRGVALVSL
                     VYDGLLGLALLAGRGLLQGWLGVGAPDPPIHADLNGLFALAVAAGYLLPYRDPQRYRA
                     YLWVMGPFLKGSGALLFTADRVLRGSPASFLLFAACDGTLALVTFWALWRTRRQAASG
                     SVSPG"
     gene            complement(6430..7830)
                     /locus_tag="PLT35_03490"
     CDS             complement(6430..7830)
                     /locus_tag="PLT35_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG28319.1"
                     /translation="MPQPPIGPQHLDRRLGPLDAAAIVVSNVIGVGIFITPQIIAQAV
                     PHPIGFLAVWLGGGLLAFVGAMAYAELGTLRPRSGGEYVYLREGYGQLAAFLTGWTSF
                     VAGFAGAMAAGALGAADYLGRFLPAASDAPPLVTIPIPGVPLVVSPRAIVALTALFGL
                     AFVHVCGLGPGRIVQNTLALLKVGALVLFVAAGLSTGLGDFGHVVAAAPASASSNLPL
                     ALIVVMFSYSGWNAAAYVAEEIRDPGRNVPRALAIGTAAVVLVYLGLNLLYVYAVPMP
                     EIGAAKSEIAMVAADRLLGPGAARLLGGLAVVVLLGSLSAMTTAGPRVYFAMARDGAF
                     FQAAARIHPRFHTPWIAIMAQAAWSAVLVLTNTKDQLAEYTGFAVLLFSAFAVSTVFV
                     LRRRNPGEPRPFKAWGYPVAPAIFVTAALAVTAASVTGRPGPSLWGALIIAAGVPAYA
                     WFRRSAPRRPPAVIPD"
     gene            7856..8584
                     /locus_tag="PLT35_03495"
     CDS             7856..8584
                     /locus_tag="PLT35_03495"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOG28320.1"
                     /translation="MLPALIAGCWMMVASMTPSCCSPDGAPPQDPWVKARRAMVADQV
                     AARGVSDPRVLDAMRAVPRHEFVPGSLREDAYWDSPLSIGYGQTISQPYIVALMTELA
                     RPAPGDRALEVGTGSGYQAAVLSRLVARVFSIELLEPLARSAAATLQRLGCTNVTVRQ
                     GDGYLGWPEEAPFDIILVTAAPEEVPQALVDQLKPGGRLVVPVGPVHDVQDLRLLVKD
                     ESGRVLSRSVIPVRFVPLVKRRGS"
     gene            complement(8654..9151)
                     /locus_tag="PLT35_03500"
     CDS             complement(8654..9151)
                     /locus_tag="PLT35_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28321.1"
                     /translation="MSAYVTRAAAGAVLAAALTLAGCSEPTVDLATSVTVSDLTTGWF
                     DVGVVDGLNKLVPSASFTVTNTGTHKLSALQLNSVFRFEGEEQELGSAFVILRGADAL
                     APSATSKPITVRGTWGFTGEQPRAQMLMHAQFKDARVQIFAKYGSAPPVKIKEAKIVR
                     QLLTK"
BASE COUNT         1303 a         3332 c         3267 g         1359 t
ORIGIN      
        1 tgttcgacgg caggaagccc agcagctccc ggatgagcgc aaggcactcc cggtcgtcct
       61 ccgccgcgaa gtgggcgacg ccgctgcgct cgttgtgcgt catggcgccg ccgagctcct
      121 ccttcgtgac gtcctcgtgg gtcaccgtct tgatgacctc ggggcccgtg acgaacatgt
      181 aactcgtctg cttcaccatg atgttgaagt cggtgatggc gggcgagtac accgcccctc
      241 cggcgcaggg gcccatgatg gcggagatct gcggcacgac gccggaggcg agggtgttgc
      301 gcaggaagat gtcggcgtag cccgcgagcg acatgacgcc ttcctggatg cgcgcgccgc
      361 ccgagtcgtt caatccgacg atgggcgcgc ccatccgcac cgccaggtcc atcaccttgc
      421 agatcttcgc ggcgttcgtc tcggagagcg agccgccgaa caccgtgaag tcctgggcga
      481 acgcgaagac cggccgcccg ttcacccgcc cgtgccccga cacgacgccg tctcccggga
      541 cgagctgctc ctgcatgccg aaatcgaggc accggtgcgt gaccagcttg tcgagctcct
      601 cgaaggtgcc gggatcgaaa agcgcctcca tgcgctcgcg ggccgtcagc ttcccggcct
      661 cgcgctgccg cttcaggcgc gcctcgcccc cgcccagctc ggcgcgccgc cgcaggtcgg
      721 ccaggcggtc gagcggcgac gtcacggccg ggctcccttc gcagccgccc agtccctcag
      781 gaaccgctcg atgccgacgt cggtgagcgg gtgcttgaac agcgcctcga tgaccgccgc
      841 cgggcacgtg cagatgtgcg cgcccagccg ggcggcctgc acgacgtgca tcgggtggcg
      901 cacgctcgcc acgatgatct cggtctggta gccgtagttc tcgtagatct cgacgatctg
      961 cgcgaccagt tccatgccgt cggtcgccac gtcgtcgagc cggccgacga acgggctcac
     1021 gaacgacgcc ccggccttgg ccgccagcag cgcctgcgcc ggcgtgaaca ccagcgtgac
     1081 gttcacgccg atgccgtcct cgcgcagcgc cttgcacgcc tgcacgccgt ccttgccgaa
     1141 cggcaccttg atgacgacgt gcgggtcgat cgccgccaga tcccggccct cgcgaatcat
     1201 cccctccgcg tcggtggcga gcacctcggc gctcaccggc ccccggacga tctcgcagat
     1261 ccgcttcgtg atcgcgcggg gatcaccctc ctccctggcc atgagcgacg gattggtggt
     1321 cacgccgtcg atgatgccga tggcgcagta ggcctcgatg tccctgatgt tgccggtgtc
     1381 gagaaagagc ttcatgtgca cctcactctg gacgtccgtt cccggggtcc gggccccggg
     1441 cttcgcgccg cgggcgtccg acgtcggacc tggtcagtcc gcctgccgca ccgggttcgt
     1501 cagcgctccg accccttcga ccgcgtcggc cacgacgtcg cccggctgga ggccgccgat
     1561 gcccgacggc atgccgggcc tcgccctgcc attcggtcag gcagacgcgg cccatcaccc
     1621 tgcccttccc gggccgggag ctccggagcg cggggctcct gcccccatgc ggcctcctgc
     1681 ttcctgcgct acctcgccgt ctcctcgacc gtgttggaca gggcgctgcg attgcccggc
     1741 gcggcggtgt ccacggccgc cacggcgtac acgtaccgga cgcctggctt cgccgacgcg
     1801 tcccggtagg tcgtctccat gacaggcgcg gtgaacaggg gctccagggc cgcgctcccc
     1861 gcctcgcccc gcagcacgat gtagcccgca agatccggtg cggacaccgc cgtccagatg
     1921 aggctcaccg cgccctcgcc cgccacggcc gccaggccgg acggcgccgg gggcggaaac
     1981 gtgtcgacgg gcgtaacgca cgcggccagc gacggctcgc tctcaaccga cgcggcgccc
     2041 ctgacgagga ccgcgcggac gtcgtagcag cgctcggcgc cgtaaggcgc gttcgcgtcg
     2101 gtccacgtca ggcgcttcac cggcgtgtcg gtgagcctca ccgatccaag cgagatcacg
     2161 taggacagcg ccggcgccga ttccatgccg aacgtgctcg cggccagcgc gtcatccgac
     2221 gcaggcgcct ggtagggcag gcgagcgccc ggcggcacgg cccagcgcac gacgacgcct
     2281 tcttcggtca cggagagcgc cggctgctcc ggggcgggcg gaggcggaac gagcggcacc
     2341 gcgggacgcg gagacgcgcc gccgcggttg cccgagcggc tgacgccgta caccaggtag
     2401 tagcggtttg ggatcgccgc cgcggccggg ccgcccagcg gcggcggcgg cggcggatcg
     2461 agccacgcgc gcggctcagc gggcgcccgc accgtcttcc ggcggccgtc gggcgggagc
     2521 ggggcctgca tgtcggccgt cagcgtctcg gtgatcgtga cgagggcccc ctggtccgcg
     2581 gccgagtcgc cgcgcggggc gggctcggcc ggccgggacg ccgcgccggg cgcgagggct
     2641 gccggcttcc gcacgggaat cttcgcgacg agcgtcatgc gcgccgtgtc ccgcacgtcg
     2701 ttctgggcca tcgccgtgta cgcatagacc tcgagcgccg ccaggtccgc cggccgcgtg
     2761 tcgtcgatgt tggccgacgg cacggtgaac gtgagggtga cgaggtcgcc cgaccgccgc
     2821 gcctgcaggt ccatcgcgcg cccgggcgcc ctgacgaagg gcgcaagcgg cgggcccttc
     2881 ttgccgcagc ccggccacgc gagccctgcc gcggccacgc acaccaccag ggcgactcgg
     2941 ctgggtctca caggatgtac cggctcaggt cctcgttctt caccgcgtcg gccagcatgc
     3001 gatcgacgta cgcggcgtcg atcgtaatcg atctcccggc cagatcgggc ccctcgaacg
     3061 agacctcgtc gagcagcttc tccatcaccg tgtgaaggcg ccgcgcgccg atgttctcgg
     3121 tgcgctcgtt cacgagcgcg gccagttcgg ccagacgatc gatggcgtcg tcggtgaagt
     3181 gcagctcgac gccgtcggtc gccagcagcg cggtgtactg ggtgacgagc gcgttgcggg
     3241 gctcccggag gatgcggacg aagtcgtccc gcccgagggc ctccagctcc acgcggatcg
     3301 ggaaccggcc ctgcagttcg ggaatcaggt ccgacggcct cgacacgtgg aaggcgccgg
     3361 cggcaatgaa caggatgtgg tcggtgcgca ccatgccatg cttcgtgttg acggtcgtgc
     3421 cctcgacaat cggcaggatg tcccgttgca cgccttcccg gctgacgtcg ggaccgtggc
     3481 cgccctcgcg ccccgcgatc ttgtcgatct cgtccacgaa caggatgccg ccctgctcga
     3541 ctcgctcgat cgccgtccgc gccacgctct cgacgtccac gagcttctgc tgctcctcct
     3601 cgacgaggtg ggcgcgcgcc tcggggacct tcatcgtgct cgagcgccgg cggccctgga
     3661 acatcccggg cagcatgtcc ctgaggttga gcccgagctc ctccatcgac gagccgccca
     3721 cgacctgcat catcggcagg gccgactccc gcacatccac ctcgacggtg cggtggtcga
     3781 gcttcccttc gcgcagctgc gcgcggaggc gctcgcgcgt ctgcgcggcg ggatcgacgg
     3841 cggggtgccc ctcggccgag ggcgggcgcg gcggcagcag gagatcgagc aggcgctcct
     3901 cggccagctg ctcggccttg tggcgcacct cgcggacgcg ctcctcgcgg accatcccga
     3961 cggcaatctc gaccaggtcg cggatcatcg actcgacatc gcgcccgacg tagccgacct
     4021 cggtgaaccg cgacgcctcg accttgagga acggcgcacg cgcgaggcgg gccaggcggc
     4081 gcgcaatctc ggtcttcccg acgcccgtcg gcccgatcat caggatgttc ttcggcgcca
     4141 cctcctcggc catctcggcc gggagctgct ggcgccgcag ccggttgcgc agcgcgatgg
     4201 cgacggcgcg cttggctctg tgctggccga cgacgtactt gtcaagctcc gacacgatct
     4261 gccgaggtgt cggggccgag gcgagggcgt ccggggaagc cggtggaagc agcggcatgg
     4321 ggtgtcggcc gcgacgtgtc aggacgtcgg ggtcagctcc tcgatggaga gattcgcatt
     4381 ggtgtagatg cagatcccgg cggcgatgcc catcgcgatc tccgcgatga cgcgggcctc
     4441 gatctgcgtg tggcgcgcca gggcccgggc cgccgccacc gcgtacaagc cccccgagcc
     4501 gatgcccacg atcccgtcgt ccggctcgat gagatcgccc gtgcccgaga gcaggaacgt
     4561 cgcgcgctcg tctgcgacga tgagcatcgc ctcgagccgc ctcagggccc ggtcggtccg
     4621 ccagtccctc gcgagttcga cggcggagcg ctcgaggttc ccgcggtact gctccagctt
     4681 cccctcgaac cgcgaaaaca gtgcgaacga gtcggccgcc gagcctgcga atcccgccag
     4741 caccttgtcg gcgtacagcc gccggatctt gcgcgccccc tgcttgacaa tggcgttgcc
     4801 gagcgtcacc tggccgtcgc acgccatgac gacccggccg tggtgccgga ccgcgaggat
     4861 cgtggttgag tgggtcatcc agagcatagt gtcaagaaaa cgggacgggg gcaacacatc
     4921 gatggcatcc ggactggaca actcggcggc cggagcgtag atttggtgag gcgttccagg
     4981 caccgccgct ggcagcgaag ttgcagcttc agggggagga ggagtccgac catgagacgg
     5041 ctcgcgttct ccggtgtcgt gttcgcggcg ttgatggcgg cgctgctcgt tgccggcccc
     5101 tcaggcgtct tcgcccagtc gccgcgcggg cgcggcgcag gccagggcgg aagcgcagcc
     5161 cagccgcggg gcggcggcgc agcggtctcg cccggcggag cttcgagcgg cggcggctcc
     5221 cggcagccga gctcagcggc tccccgcagc ggacgagccg gcggcggcaa cgccggcagc
     5281 agcgccggcc gcgaggccag gccccgcgat ggcggcggca gctggcgctc gcccgctgac
     5341 ggccaggcag ccgggcgcgc ccgcgacgcc ggcggcgcgg acgtcgtggg cgtcccgtgg
     5401 tattcccgcc cgcgcggcgg caatccctcc acgggcaccg ccattccgcg cgccgatgcg
     5461 ccggcccccc agggcggcgg cgggtactac gttttcaacc agaactacta caacggctac
     5521 tacggataca gatacaaccc gttcaactgg tacggcaacg tctggggcta tggcgcgttc
     5581 ggtctcggct atttctatta cgacccgttc tggtggtcgt atgaggacta ccgctaccgg
     5641 aactacggcg gctactccag cctgccctac ggctacgggt acggctacgg gccggcgggc
     5701 tacggcgacg gatacgcgac gggcactcgc gagccgatgg gcggcgtccg cctcaaggtc
     5761 aagccgaaca cggcctccgt ctacgtagac gggtattacg cgggccgcgt cgatgacttc
     5821 gacaacatgt tccagaagct ccaactgccg ctcggcctgc accgcatcga gatcgcggcc
     5881 cccggctaca aaccgctcgt cttcgagctg aacatccgga gttgggacac gatcacttac
     5941 gaagcgtggc tcgaaccgat cgccaggtag cggcggccgt ggagcgttcg tcccgcgcga
     6001 ccggcgagcc ccgggtggcg atgcccgggg cttcgttgtc tccgtcggtc ctgcgcggcg
     6061 tggcgctggt ctcgctcgtc tacgacggcc tgctgggact ggcgctgctg gcgggccgcg
     6121 gcctgctgca gggctggctc ggcgtgggcg cgcccgatcc gccgattcac gccgatctga
     6181 acgggctgtt cgccctggcg gtcgccgcgg gctacctgct cccctaccgc gacccgcagc
     6241 gctaccgcgc ctacctctgg gtgatggggc cgttcctcaa gggctcgggg gcgctgctct
     6301 tcacggccga ccgcgtgctc cgcggctcgc ccgcgtcatt cctgctgttt gcggcgtgcg
     6361 acggcacgct cgcgctcgtc acgttctggg cgctgtggcg cacccgccgg caggccgcgt
     6421 ccgggtccgt cagtccggga tgacagcagg cggccgccgt ggggcgctcc gccggaacca
     6481 cgcgtaggcc ggcacgccgg cagcgatgat gagcgcaccc caaagcgacg gcccaggccg
     6541 gccggtcacg gacgctgccg tgacggcgag cgcggccgtc acgaagatcg cgggcgcaac
     6601 cgggtacccc cacgccttga acggtctcgg ctcgccgggg ttgcgcctgc gcagcacgaa
     6661 gaccgtgctc accgcaaacg cggagaacag cagcaccgcg aaccccgtgt actcggccag
     6721 ctggtccttc gtgttggtga gcacgaggac ggcgctccag gccgcctggg ccatgatggc
     6781 gatccacggc gtgtggaagc gggggtggat gcgggctgcc gcctggaaga acgccccgtc
     6841 gcgggccatg gcgaaataga cgcgggggcc cgccgtcgtc atcgcgctca ggctgccgag
     6901 cagcacgaca acggcgaggc cgccgaggag ccgggcggcg ccggggccga gcagccggtc
     6961 tgccgcgacc atcgcgatct cggacttcgc cgcgccgatc tcgggcatcg gcacggcgta
     7021 gacgtaaagc aggttcaggc ccaggtacac cagcaccacc gcggccgtgc cgatggccag
     7081 ggccctgggc acgttgcgcc ccgggtcccg gatctcttcc gccacgtagg ccgccgcgtt
     7141 ccagccgctg tacgagaaca tcacgacaat gagggcgagc ggcaggttgg acgaggcgga
     7201 ggccggcgcc gcggccacca cgtgcccgaa gtcgcccaag ccggtgctga gccccgccgc
     7261 cacgaagagc acgagcgctc ccaccttcag cagcgcgagg gtgttctgga cgatccggcc
     7321 gggcccgagc ccgcagacgt ggacgaaggc gagtccgaac agggccgtca gggcgacgat
     7381 cgcgcgaggc gagacgacga gcggcacgcc cgggatcggg atggtgacga gcggcggcgc
     7441 gtcgctggcg gccggcagga agcgtcccag gtagtcggct gcgcccaggg cccccgccgc
     7501 catggccccc gcaaacccgg ccacgaacga ggtccatccg gtgaggaacg cggcgagctg
     7561 gccgtacccc tcgcggaggt acacatattc cccgcccgac ctcgggcgca gggtcccgag
     7621 ctcggcatag gccatcgcgc cgacaaacgc gagcaggccc ccgcccagcc acacggcaag
     7681 aaacccgatc ggatggggca cggcctgtgc gatgatctgc ggcgtgatga agatgccgac
     7741 gccgatgacg ttcgagacga cgatcgcggc agcgtcgagc ggacccagcc ggcgatccag
     7801 atgctgcggc ccgatcggcg gctgtggcat cccggtatta tagctggtgc ctcggatgct
     7861 ccccgcgctc atcgccggct gctggatgat ggtggcatcg atgacgcctt cctgctgctc
     7921 gccggacggc gctccgccgc aggacccgtg ggtcaaggcg cgccgggcga tggtggccga
     7981 ccaggtggcg gcccgcggag tatccgatcc gcgcgttctc gacgcgatgc gcgccgtgcc
     8041 gcgccacgag ttcgttccgg gttccctccg ggaggatgcc tactgggaca gcccgctctc
     8101 gattggctac ggccagacga tctcgcagcc ctacatcgtg gcgctgatga ccgagctggc
     8161 gaggcctgcg ccgggcgacc gggcgctgga agtcgggacg ggatccggct accaggcggc
     8221 cgtgctgtcc cgcctggtcg cgagggtgtt cagcatcgaa ctgctcgagc cgctggcccg
     8281 atcggcggcg gcgacgctgc agcgtctggg ctgcaccaac gtgacggtcc gccagggcga
     8341 cggctacctg ggctggccgg aagaagcgcc cttcgacatc atcctggtga cggcggcgcc
     8401 ggaggaggtc ccgcaggcgc tcgtcgatca gctgaagccc ggcgggcggc tcgtcgtgcc
     8461 ggtgggcccg gtgcacgatg tgcaggacct gcggctgctc gtgaaggacg agtccgggcg
     8521 ggtcctctcg cgcagcgtga ttccggtgcg cttcgtcccg ctcgtgaagc ggcggggctc
     8581 ctgaacgaga aacccgcgcg tgagccgggc tcgaacccgc ccctcccgcc ccgggcgcgg
     8641 ggcgaagccg gcgctacttc gtcaacagct gcctgacaat cttggcttcc ttgatcttca
     8701 cgggcggcgc cgatccgtac ttggcgaaga tctggacgcg cgcgtccttg aactgcgcgt
     8761 gcatcagcat ctgggcccgc ggctgctcgc cggtgaagcc ccatgtgccg cgaacggtga
     8821 tcggcttgct cgttgccgag ggagccaggg cgtcggcgcc ccggaggatc acgaaggcgg
     8881 acccgagttc ctgctcctcg ccttcgaacc tgaagacgga gttgagctgg agggcgctca
     8941 gcttgtgcgt ccccgtgttc gtcacggtga aggaggcgct tggcacgagc ttgttcaggc
     9001 cgtcgacgac gccgacgtcg aaccagcccg tcgtgaggtc gctcacggtg acggacgtgg
     9061 cgaggtcgac cgtcggttcc gaacatccgg ccagcgtcag ggcggcagcg agcaccgcgc
     9121 cggccgccgc ccgcgtcaca tacgcgctca ttgggctgcc ctttcgttcg ctccggctcg
     9181 aggccgggga cgccgaagct gctccactct atcagtattc acgcgactcg acgcgtttgc
     9241 gggcctcctc cggcccgatg c
//
LOCUS       DAOSIF010000059         9245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7121_length_9245_cov_4.378890, whole genome shotgun sequence.
ACCESSION   DAOSIF010000059 DAOSIF010000000
VERSION     DAOSIF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7121_length_9245_cov_4.378890"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="murJ"
                     /locus_tag="PLT35_03505"
     CDS             complement(<1..1044)
                     /gene="murJ"
                     /locus_tag="PLT35_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOG28322.1"
                     /translation="MPEEPPTPDSAPPQATASQITKSAGVIGAATMGSRVLGLVRDQV
                     LAFLFGAGDQMDAYNIAFRIPNLVRDLFAEGAMSAAFVPTFTRTLTVSGRAPAWRLGN
                     LVVNALLLATGAIVILGIVFASPLSRLFASAYAEVPGKLELTIFLSRVMFPFLLLVAV
                     AAALMGMLNSLHRFFVPAMSPAMFNVGSIVCTIGLAPVMPLVGMPIILAPAIGVLVGG
                     LLQMAIQWPALRREGYRYEPILDVRDEGLRRVLTLMGPGVVGLAAVQINLLVNSILAT
                     GEGTGAVSALSYAFRVMYMPIGIFGVSIATAVVPTLSRHAARHDAAGMRETISAGLRL
                     MLMMNVPATVGLMA"
     gene            complement(1436..1885)
                     /locus_tag="PLT35_03510"
     CDS             complement(1436..1885)
                     /locus_tag="PLT35_03510"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG28323.1"
                     /translation="MSMLADVNARITQAMKAHDAGTLSAMRMLKAALTNREIQKGQAL
                     DDAESIQVLASLIKQHRDSIEQFARGGRQDLVAREQAELTLLEALMPPPVSPAVIDEA
                     VARAIAETGAASPRDMGRVMKAAMATLAGKGVDGKTVNEAVRKRLGA"
     gene            2037..4037
                     /locus_tag="PLT35_03515"
     CDS             2037..4037
                     /locus_tag="PLT35_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28324.1"
                     /translation="MPMPLLTRFSRGPAWTTAAFAAFLAAAAGALAVVAAFQRHGSIR
                     VDASRGIPAFVTGLYPTERQGKDYFAWSGGRVSVRAGLADRRAAWQCQARLVNWRPPS
                     AGPADVRVEADGRVLLERRVSEPRASLDFAVPPVPARRDLDFEIVVSPTFVPGPQDPR
                     ELGLAFEGLRCDPAAGAWAWPARQVVARASLSAALAGLVLGIAGLPAGAAAGFAVLVA
                     AAQGFSLASGAAAHLPGRPPVFLLAGLLAVFCLLPILAARFVRPQGLSTAARLAVAVS
                     ACACCLKLVFLLHPDKTLVDAVFHAHRFEMVLAGRFFFTQLSTSATPFPYAIGLYVFA
                     APWAALTADHVTLLRTVVCACEAAAVLLLYPLVARAWGDRAAGVAAVALYHLAPLPIA
                     VLGNANLTNHFGQSVAVAAMAAAAGWSFAGRRAAETVGLALVAGLAFLSHVGTFAVLL
                     PTLLVLALATRGLGRDAGRIPARCILAATALAVALAVGLYWGRFFDVYEPHIASAKAE
                     LASAFKRGSSAAAPAAHDAGGVPAARTTPLQLGFSGAVRQTRGAFGLPLIALAVAGAS
                     SLARRRRADRLVAALAAWFAAWAGFLLWSALRRVEPPYVQDAWEFIGRVEMATTPAVA
                     ILAACGAAWAWRSGRLLRAVSAGLVLAALAGGARAVVSWILF"
     gene            complement(4034..4768)
                     /locus_tag="PLT35_03520"
     CDS             complement(4034..4768)
                     /locus_tag="PLT35_03520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG28325.1"
                     /translation="MADDRPSVRRGEYASRGDYHRVLDEAWDYRPTYLAKMAAVRRHL
                     DTLPPGTRVLDAGCGEGVLVQDYAGRLDIEGVDLNCSGDRIRTGSVTALPWPGGAFEQ
                     VLCLDVLEHLAFGEQDAALSEIFRVLRPCGLALLSVPNLAHLQSRAHFLLTGRLIRTA
                     SPAKHPGDRPAGEYLALARSAGFDVVRRRGIFPTVPVLTALVRRHPVSLQWLHAALTR
                     AIPVPGWCFLNLIWLRKPAAAAGRSA"
     gene            complement(4776..>5774)
                     /locus_tag="PLT35_03525"
     CDS             complement(4776..>5774)
                     /locus_tag="PLT35_03525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28326.1"
                     /translation="RALPGPVTWIESRARGRRVPGLSQISSLLNAARIGRASRGALSA
                     ADVVHVHSNGLLPEVAGLLASRAGTPVVLTLYGTEIWHYRARRGGLDLFTRMYRAARH
                     VTFYSGALRDRAVELGLTRGGMSVIYPPVAGYFARAGEGERRAARVELGLRESNVLVN
                     VKRLHPLAGQRRLLDAMPSVLRAHPDTRLVICGTGPLRDELAAQAAALGVSERVTFAG
                     LVDNRTVARYQQAADLFVLPSELEALPTVAVEALAAGTPVVSTDNPGGVELGRLFGDD
                     VRVVDRSQPGELASAIVEFLAAARRTRPSSDAVLEREFSPAVVAERFAQVYRGVLQ"
     assembly_gap    5775..5784
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5970..6947)
                     /locus_tag="PLT35_03530"
     CDS             complement(5970..6947)
                     /locus_tag="PLT35_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28327.1"
                     /translation="MLHLLVGIDTEGDNQWSAEARLNQAFENIYALPALHARFQRRGV
                     RPTYLITWPVARDPRSASVLRELRSGGDCEIGAHHHAWETPPCTPDDVRRHPYALQLP
                     REQFAAQVASLTGAIADAVGEPPRSYRSGRFGFSIAHVPDLERAGYRVESSLAPLFYE
                     GHKGGPDFVSVPPSPYFLSYDDPAVPGSSDLLELPVTAALNRRVPAFVERLYARAPSP
                     YQTKRVLRKLGVARVQWLRPSYSPLADMCAVARRMKDDGVPILNLIFHSSEAIVGGSP
                     YNRTARELDAFFDRLDEVLAFAVRDLGAVPATFSEFRDRYCAAPPLAGP"
     gene            complement(6950..7993)
                     /locus_tag="PLT35_03535"
     CDS             complement(6950..7993)
                     /locus_tag="PLT35_03535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG28328.1"
                     /translation="MATIDTFRPEDERQITAMYRRVFGDAAAEANRLRWDWQYARNPN
                     RPPDGPQIWVAREGPAVIGQYAAMPVRLMAGGREIDASWGMDVMVAPERQRQGLGEVL
                     FRTWEQHVGAALGLGLSESSYRLFRKLRWPNVGPVPCFVKPLTRRALRRSSWPTPVNR
                     VVSALTLPLVRAAWRPRPLRANVESIQRFGDEFTALWERVAPRFAFAVRRDAAYLNWK
                     FREAPHVRYAAVAARRDGALRGYVVYRHLQEARARVTLLVDFLADPGDAPSFETLLGW
                     VDREAREANSDKIRAFNLHAGFARSLKRSGYFPVKSTMEFTAKINAVQLPPSFYEDTS
                     GWHVTLGDSDQDR"
     gene            complement(8005..8847)
                     /locus_tag="PLT35_03540"
     CDS             complement(8005..8847)
                     /locus_tag="PLT35_03540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG28329.1"
                     /translation="MRDIDTDAIKSHAARRFRSEYDYALFEYYRSAKVITYLERAGVT
                     LGGRVLDAGCGGGGMPLSLAEEADRVVGIDLAPRFRDAGRRLASERGLANLHFAQADG
                     QALPFADASFDLVFSHAVIEHVADAARYLRELARVLRPGGRMYLSTAPYLSFAGAHLP
                     RLKIPVPIHLLVGRRAAFGLFVRLARHAPWLLQEPEHENSFIALARQGRLKDDDLLEK
                     VRVPRLREHIRRAGLSIVAETLQLTGTFRRAPAPLVRWLREAPVIQDIVVGNIEYVLA
                     RQEP"
     gene            complement(9009..>9245)
                     /locus_tag="PLT35_03545"
     CDS             complement(9009..>9245)
                     /locus_tag="PLT35_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28330.1"
                     /translation="VVTRRRADVLADLASRLDGRVFTHVILTVDPESPRGRGWYANSN
                     LGWPVVSRILANYEPAGVTAGLRLYAPRARAPEP"
BASE COUNT         1264 a         3303 c         3369 g         1299 t
ORIGIN      
        1 cgccatcagg ccgaccgtgg ccggaacgtt catcatcagc atcagccgca gcccggcaga
       61 gatcgtctcg cgcatgccgg cggcatcgtg cctcgccgcg tgccggctga gcgtcggcac
      121 cacggccgtc gcgatggaca cgccgaagat cccgatgggc atgtacatca cgcggaacgc
      181 gtagctcaac gccgacacgg cgccggtgcc ttcccccgtc gccaggatgc tgttcaccag
      241 caggttgatc tgcaccgcgg ccagcccgac gacgcctggc cccatcagcg tgagcacgcg
      301 gcgcaggccc tcgtctcgga cgtcgaggat gggctcgtag cggtacccct cacggcgcag
      361 cgccggccac tggatcgcca tctgcaggag cccgccgacg aggacgccga tcgccggggc
      421 caggatgatc ggcatcccga cgagcggcat caccggcgcg agcccgatcg tgcacacgat
      481 cgaccccacg ttgaacatcg ccggcgacat cgcgggcacg aagaaccggt ggagcgagtt
      541 cagcatgccc atgagggccg ccgcgacggc cacgagcagc aggaacggga acatgacccg
      601 gctcaggaag atcgtcagct ccagcttccc cggcacctcc gcatacgcgc tcgcgaacag
      661 cctgctcagg ggcgacgcga acacgatgcc caggatcacg atggcgccgg tggccagcag
      721 gagggcgttc acgaccaggt tcccaaggcg ccacgcagga gcgcggcccg aaaccgtcag
      781 ggtgcgcgtg aacgtcggca cgaacgccgc gctcatggcg ccctcggcga agaggtcgcg
      841 cacgaggttc gggatgcgga acgcgatgtt gtaggcgtcc atctggtcgc cggcgccgaa
      901 gaggaacgcc aggacctggt cccggacgag gccgaggacg cggctgccca tcgtggcggc
      961 gccgatgacg ccggccgact tcgtgatctg cgatgcagtg gcctggggcg gcgcgctgtc
     1021 tggcgtgggc ggctcttcgg gcatgtggaa ccgccagcgt accacattgg acatgcccat
     1081 caggaaggcg ggcacccgcc agtcgggatg gcgggtgccc tttgggagga gagaggagtc
     1141 tatcgggtct tcatcaactt agacgacagc ggcggttgat tggtttggct tcgccgggct
     1201 gagaggagcg ttggcgcgaa gcgccgccga gaccgtggtc ttcgcttttg tttcgtccct
     1261 tcgtcagcct ctcttctgag acacgccgcg ccccgaacca tccgcggcga gaacgaagcg
     1321 ctgcgcatcg cgagaaacgc tctcctgcgg ccggtcgcgg aaggcgactc gacgctgtac
     1381 gaacgcctga gggccggcgg gagccgcgcg gccggcgagc ccgggcagga gccgatcagg
     1441 ccccgaggcg ctttcggacc gcctcgttga cggtcttccc gtccacgccc ttgcccgcca
     1501 gggtcgccat cgcggccttc atcacccggc ccatgtccct gggggaggcg gccccggtct
     1561 cggcgatggc cctggcgacg gcctcgtcga tgacggcggg gctgacgggc gggggcatga
     1621 gcgcctcgag cagggtgagt tcagcctgct ccctggccac gaggtcctgc cgcccgcccc
     1681 tggcgaactg ctcgatcgag tcgcggtgct gcttgatgag gctcgcgagg acctggatcg
     1741 actcggcgtc atcgagcgcc tggcccttct ggatctcgcg gttcgtcagg gcggccttga
     1801 gcattctcat ggcggacagc gtcccggcat cgtgcgcctt catggcctgg gtgatccgtg
     1861 cgttgacgtc agcgagcatg gacatcggca ctctccgttc gcggcggcag acacgatcct
     1921 agcgcatgtc gcgcgggccc gcgtggcggc ggtcccgatg aggctcgccg atcgcgggcc
     1981 cgccggcccc gatggccagc gggcgccgat cccgtgtacg atgcgggacg ctgcgcatgc
     2041 ccatgccgct cctgacgcga ttctcccgcg gccccgcctg gacgaccgcg gccttcgccg
     2101 ccttcctggc ggccgcagcc ggggcgctgg ccgtcgtcgc tgcctttcag cgccacgggt
     2161 ccatcagggt cgacgccagc cgcgggatcc ccgctttcgt caccggcctc tatccgacgg
     2221 agcggcaggg caaggactac ttcgcgtgga gcggcggccg ggtatccgta cgcgcggggc
     2281 ttgccgaccg ccgggccgcc tggcagtgcc aggcacgcct cgtcaactgg cgcccgccct
     2341 cggcggggcc agccgacgtg cgcgtcgaag cggacggccg ggtgctgctc gagcggcgcg
     2401 tcagcgaacc gcgcgcctcg ctggacttcg cggttccgcc cgttcccgcc aggcgggacc
     2461 tcgacttcga gatcgttgtc tcgccgacgt tcgtgccagg accccaggac cccagggaac
     2521 tcgggctggc cttcgagggg ctgcgctgcg atccggctgc cggcgcctgg gcatggcccg
     2581 cgcgccaggt cgtcgcgcgc gcgagcctgt cggccgcgct tgccgggctc gtgctcggga
     2641 tcgcgggtct gcccgctggt gcggcagccg gcttcgccgt cctcgttgcg gcggcacaag
     2701 gcttctcgct ggcgtcggga gccgccgcgc acctgccggg ccgccccccc gtgtttctgc
     2761 tggccgggct gctggcggtg ttctgcctgc tcccgatcct ggcggcccgc ttcgttcgcc
     2821 cgcaggggct gagcacagcc gcccgcctcg cggttgccgt gtcagcctgc gcgtgctgtc
     2881 tgaagctggt cttcctgctg catcccgaca agaccctggt cgatgcggtc ttccacgcgc
     2941 accggttcga gatggtgctg gccggccggt tcttcttcac gcagctgtcg acgagcgcga
     3001 cgccgttccc ctatgccatc ggcctctacg tgttcgcggc cccttgggcg gcgctcacgg
     3061 cggaccacgt caccctgctc cggaccgtcg tctgcgcctg cgaagccgcg gccgttcttc
     3121 tgctctatcc gctcgtcgcc cgcgcctggg gcgaccgcgc ggccggcgtt gccgccgtgg
     3181 ccctgtatca cctggcgccg ctgccgatcg cggtcctggg gaacgcgaac ctgacgaacc
     3241 atttcggaca gtcggtcgcg gttgccgcga tggcggccgc ggcaggctgg tcgtttgccg
     3301 gccgccgggc cgccgagacc gtcggcctgg ccctggtggc gggactggcg ttcctgtccc
     3361 atgtcgggac gttcgccgtc ctgctgccga cgcttctggt cctggccctc gccacgcgcg
     3421 gccttggccg cgacgcgggc cgcatcccgg cgcggtgcat cctggccgcc actgcactgg
     3481 ccgttgcgct ggccgtcggc ctctactggg gtcgcttctt cgacgtgtac gaaccccaca
     3541 tcgcctctgc gaaggcggag ttggcgtcgg cgttcaaacg cggttcgagc gcggctgcgc
     3601 cggcggcgca cgacgcgggc ggtgttccag cggcgcggac cacgccgctc cagctcgggt
     3661 tctccggcgc cgtcaggcag acgcgcgggg cgttcggcct gcccttgatc gcgctggctg
     3721 tcgcgggcgc gtcgagcctc gcgcggcggc ggcgcgccga ccgtctcgtc gcggcgctcg
     3781 cggcgtggtt tgccgcgtgg gcggggttcc tcctctggag cgcgcttcgt cgggtcgagc
     3841 ccccgtacgt gcaggacgcg tgggagttca tcgggcgtgt cgaaatggcc acgacgccgg
     3901 ccgtcgccat cctcgcggcg tgcggggccg cctgggcgtg gcggtccggc cgcctcctgc
     3961 gggccgtttc tgcgggactc gtgctggccg cgctggccgg aggcgcgcga gccgtcgtca
     4021 gctggatcct cttctaggcc gaacgccccg ctgcggcggc cggcttccgc agccagatca
     4081 ggttcaggaa gcaccacccc ggcaccggga tcgcccgcgt cagcgccgcg tgcagccatt
     4141 gcagcgatac cgggtgccgc ctcaccaggg cggtcagcac gggcaccgtc gggaagattc
     4201 cccgccgccg gaccacgtcg aatccagccg accgggccag ggcgagatac tcgcctgccg
     4261 gacggtcgcc ggggtgcttg gccgggctgg ccgtgcgaat gaggcgtccc gtcagcagga
     4321 agtgcgcgcg cgactgaagg tgcgcgaggt tgggcaccga caggagggca aggccgcatg
     4381 gccgcaggac ccggaagatc tcggacaagg ccgcgtcctg ctcgccgaac gctaggtgct
     4441 ccaggacgtc caggcacagc acctgctcga acgcaccgcc gggccacggg agggccgtga
     4501 cggatcccgt ccggatgcgg tcgccgctgc agttcaggtc gacgccttcg atgtccaggc
     4561 gcccggcgta gtcctgcacg aggacgccct cgccgcagcc ggcgtccagg acgcgggtgc
     4621 ccggcggcag agtgtcgaga tggcgccgca cggcggccat cttcgcaaga tacgtaggcc
     4681 ggtagtccca cgcctcgtcg agcacgcggt ggtagtcccc gcgtgacgcg tactcgccgc
     4741 gcctgaccga gggccgatcg tctgccatgc gccgcctatt gtaggacgcc gcggtagacc
     4801 tgcgcgaacc gctccgcgac gaccgccggg ctgaactccc gctcgaggac ggcgtccgag
     4861 gacggacggg tccgcctggc ggcggcgagg aattccacga tggccgacgc gagctcgccc
     4921 ggctgagatc gatcgaccac gcgcacgtcg tcgccgaaga gccggccgag ttcgacgccg
     4981 ccgggattgt cggtcgaaac gaccggcgtg ccggcggcga gcgcctcgac ggcgaccgtc
     5041 gggagcgcct cgagctcgga ggggagcacg aagaggtcgg ccgcctgctg gtagcgagcc
     5101 accgtgcggt tgtcgacgag gcccgcgaac gtgacgcgct ccgacacgcc gagggccgcc
     5161 gcctgcgccg cgagctcgtc gcggagcggg cccgttccgc agatcacgag gcgcgtgtcg
     5221 gggtgcgccc ggagcaccga cggcatcgcg tcgaggagcc tccgctggcc cgccagcgga
     5281 tggaggcgct tcacgttgac gaggacgttc gactcgcgca ggcccagttc cacgcgggca
     5341 gcccggcgct cgccctcgcc ggccctggcg aagtacccgg cgacgggagg gtagatcacg
     5401 ctcatgccgc cgcgcgtcag gccaagttcg acggcgcggt cgcggagagc gccgctgtag
     5461 aaggtgacgt gacgggcggc cctgtacatc cgggtgaaga gatccaggcc gccccgcctg
     5521 gcccggtaat gccagatctc ggtgccgtag agcgtgagca ccaccggcgt gccggcccgc
     5581 gacgcgagca gtccggcgac ttcgggcagc aggccgttgc tgtggacgtg cacgacgtcg
     5641 gccgccgaga gcgccccgcg cgacgcgcgc ccgatccggg cggcgttcag gagcgacgag
     5701 atctgcgaga gacccgggac ccgccgcccg cgcgcgcgcg actcgatcca ggtgaccggc
     5761 cccggcagcg cgcgnnnnnn nnnnccacat ccgcgatcga ccccgcgtcg ccgctgacgg
     5821 aggcccgcgg cggatgcgcg acgaacagcg gcgtgtcgcc cgacgccgcg gcccaccggc
     5881 cgaggtgcca cggcaggagc gcgttggcgg cctgatcggg cggaaggtgc gaggtgacgt
     5941 ggcagatgcg catcggcccg gcccgcctct cagggccccg cgagcggagg cgcggcgcag
     6001 tatcggtcgc ggaactcgga gaaggtggcc ggcacggcgc ccagatcgcg cacggcgaag
     6061 gcgagcacct cgtcgagccg atcgaagaac gcgtcgagct cgcgcgccgt ccggttgtac
     6121 gggctgccgc cgacgatcgc ctcgctcgag tggaagatca ggttcaggat cgggaccccg
     6181 tcgtccttca tgcgccgggc gacggcgcac atgtcggcca gcggcgaata cgacgggcgg
     6241 agccactgca cgcgggcgac gcccagcttc ctgagcacgc gcttcgtctg gtacgggctc
     6301 ggagccctgg cgtacaagcg ctcgacgaac gcgggcacgc gccggttcag ggccgcggtg
     6361 acgggcagct cgagcaagtc gctcgagccg ggcaccgccg ggtcgtcgta ggacaggaag
     6421 tacggggacg gcggcaccga gacgaagtcc ggcccgccct tgtgcccctc gtagaagagc
     6481 ggcgccaggc tggactcgac ccggtacccg gcccgctcca ggtcggggac gtgggcgatc
     6541 gagaagccga accgcccgga tcggtacgag cggggcggtt cgccgacggc gtccgcgatg
     6601 gcccccgtca gcgatgccac ctgcgccgcg aactgctcgc gcggcagctg cagggcgtac
     6661 gggtgccggc ggacatcgtc gggcgtgcac ggcggcgtct cccacgcgtg gtggtgcgcg
     6721 ccgatctcgc agtcgccgcc gctccgaagc tccctcagca ccgaggccga ccgggggtcg
     6781 cgcgccacgg gccacgtgat gaggtacgtg ggccggacgc ccctgcgctg gaacctcgcg
     6841 tgcagggcgg gaagcgcgta gatgttctcg aacgcctggt tcagccgcgc ctcggcggac
     6901 cactggttgt cgccttccgt gtcgatgccg acgagcaggt gcagcatggt cagcggtcct
     6961 gatccgagtc gcccagcgtg acgtgccagc cgctcgtgtc ctcgtagaac gacggcggga
     7021 gctggacggc attgatcttc gcggtgaact ccatcgtgga tttcaccggg aagtaccccg
     7081 agcgcttcag cgaccgcgcg aaacccgcgt gcaggttgaa cgcccggatc ttgtccgagt
     7141 tcgcctccct cgcctcccgg tcgacccagc ccaggagcgt ctcgaacgac ggagcgtcgc
     7201 cgggatcggc gaggaagtcc accaggagcg tgacgcgggc gcgcgcctcc tgcaggtgcc
     7261 ggtagacgac gtatccgcgc agcgcgccgt cgcgcctggc ggcgacggcg gcataccgca
     7321 cgtgcggggc ctcccggaac ttccagttga gataggcggc gtcgcggcgc acggcgaacg
     7381 cgaaccgcgg cgccacgcgt tcccacagcg ccgtgaactc gtcgccgaaa cgctggatgc
     7441 tctcgacgtt cgcccggagc ggccgcgggc gccacgcggc gcgcacgagg ggcagcgtga
     7501 gcgcagagac gacgcggttg accggcgttg gccaactcga acgccgcagg gcgcgccgcg
     7561 tgagcggctt cacgaagcac ggcacggggc cgacattcgg ccaacggagc ttccggaaga
     7621 gccggtacga cgattccgac aagccgaggc ccagcgcggc gccgacgtgc tgctcccagg
     7681 tgcggaacag gacttccccg aggccttgcc gctggcgctc cggcgcgacc atgacgtcca
     7741 tgccccacga cgcatcgatc tcccggccgc cggccatcag ccggacgggc atggcggcgt
     7801 actggccgat cacggccggt ccctcgcgcg ccacccagat ctgcgggccg tcgggagggc
     7861 ggttggggtt gcgcgcgtat tgccagtccc agcgcaggcg gttcgcctcg gcggcggcgt
     7921 cgccgaacac gcgccggtac atcgccgtga tctggcgttc gtcctcggga cggaaggtgt
     7981 cgatcgtggc catggcgtcg agggctaagg ctcctggcgg gcgaggacgt actcgatgtt
     8041 cccgacgacg atgtcctgga tgaccggggc ctcgcgcagc cagcgcacga gcggcgcggg
     8101 cgcccggcgg aacgttcccg tcagctgcag cgtttccgcg acgatcgaca gcccggcccg
     8161 ccggatatgc tcgcgcaggc ggggcacgcg caccttctcg agcagatcgt cgtccttgag
     8221 ccggccctgc cgcgcgagcg cgatgaagga gttctcgtgc tcgggctcct gaaggagcca
     8281 gggcgcgtgc ctggcgaggc ggacgaagag gccgaacgcc gcccgccggc ccaccagcag
     8341 gtggatcggc acggggatct tcagccgcgg caggtgcgcg ccggcaaacg acaggtacgg
     8401 cgccgtcgag aggtacatcc ggccgccggg cctgagcacg cgcgccagtt cgcggaggta
     8461 gcgcgccgcg tcggcgacgt gctcgatgac cgcatgcgag aacacgaggt cgaacgacgc
     8521 atccgcgaag gggagcgcct ggccgtccgc ctgcgcgaag tgcaggttgg ccaggccgcg
     8581 ctcgctggcg aggcgtctgc ccgcgtccct gaagcgcggc gcgaggtcga tgcccacgac
     8641 gcgatcggcc tcctcggcga gcgacagggg catgcccccg ccgccgcagc cggcgtcgag
     8701 gacgcggccg ccgagcgtca cgccggcccg ctcgaggtag gtgatgactt tcgcgctgcg
     8761 gtagtactcg aagagcgcgt agtcgtactc ggagcggaag cgccgggccg cgtgcgactt
     8821 gatggcgtcg gtgtcgatgt cccgcacgtg tcctctgcca gcctgcgatc cctcctcgcc
     8881 acgggccgtc gtcgctccgg ccgtccggcg acaccggaac cgccggtgcg ggaatcgctc
     8941 acattctatc ccaccagtcc cagcggcgcg ggccgggacg cggcttccgg gctgggcgct
     9001 cgcgcggctc acggctccgg cgcacgggcc cgcggcgcgt agaggcggag cccggccgtg
     9061 acgccggccg gctcgtagtt cgccaggatc cgcgacacca ccggccagcc cagattgctg
     9121 ttcgcatacc agccccgtcc gcgcgggctc tccggatcga cggtcaagat cacgtgcgtg
     9181 aaaacgcgtc catccaggcg cgacgccagg tccgcgagca cgtcggcccg gcgacgggtg
     9241 accac
//
LOCUS       DAOSIF010000060         9232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7130_length_9232_cov_4.132178, whole genome shotgun sequence.
ACCESSION   DAOSIF010000060 DAOSIF010000000
VERSION     DAOSIF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7130_length_9232_cov_4.132178"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1358
                     /locus_tag="PLT35_03550"
     CDS             150..1358
                     /locus_tag="PLT35_03550"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOG28331.1"
                     /translation="MRPIRETITLEAAREIIDRVIKPMERTERVRGDLASGRVVSRAV
                     EAQADVPPFTRAAMDGYAVRAADTVGASTHEPRTLARVETVYTGQVPTKVVGRGQCTE
                     IATGAPVPDGADAVVMVEETDKDAGGAVRVFSPVYPGQNVTKQGADIRAGQLVLAPGD
                     VLNPSRIGAIAALGVIDVEVYARPRVAILSTGNEVAEPGAPLAPGHIYDINRFTLSAV
                     LRDHGAVPVPYPTAPDTIPELERAIEACSAEDLILFSGGSSVGERDLILDVVERMGEV
                     LFHGIAVKPGKPTLFGTVAGKPVFGMPGYPTSCLSNAYMLLVAPVRRMARLPEWTPRV
                     IRLPLGQRIVSTTGRHQFYTVRVVDGQAMPAFKASGDITSMSQADGYIEIPAQVDVVE
                     KGEVVALTLF"
     gene            1447..1938
                     /locus_tag="PLT35_03555"
     CDS             1447..1938
                     /locus_tag="PLT35_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1591..1593,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HOG28332.1"
                     /translation="MPRDQSTTLEAGDRAPLFTLPNQKGVPVSLAEALAKGPVVLGFH
                     RGTWUPNCRRRFGDLARNAEHYRALGATVMAVVAQRSEKVKRHIEDTGLPLDLLIDDT
                     REVVKAYGVWHQIGFDAWNISRPALFIIDRGGIVRYAFVADWQTEFPEHEVIVAELAK
                     AGG"
     gene            complement(2146..2748)
                     /locus_tag="PLT35_03560"
     CDS             complement(2146..2748)
                     /locus_tag="PLT35_03560"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOG28333.1"
                     /translation="MAHELPPLPYAFDALEPFIDTMTMQIHHGKHHATYVSNLNAALE
                     KAPELQSLSADDLVRDLSRVPESVRTAVRNNGGGHVNHAMFWQIMAPKAGGEPSGPLA
                     DAIKAAFGSFEAFKGEFKKAALGRFGSGWAWLVNTGGKLTIESTPNQDNPIMEGRQAV
                     MGIDVWEHAYYLKYQNRRADYVDAWWSVVNWSEVAKRYTR"
     gene            3032..4390
                     /locus_tag="PLT35_03565"
     CDS             3032..4390
                     /locus_tag="PLT35_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG28334.1"
                     /translation="MMSGDNSMNRRQFLRKTALAAAAASAASAPRKTAAQINNAAGVS
                     LRQVNLGFIGIGIRGTQLMEAASGIAGVQVRAAADCYKGHLDRAAELITPPPAVTGDY
                     TEILARPDIDAIVIATPDHWHLRILQEALAAGKHVYVEKPMTHAWEEGEAFISAVEKS
                     GKVVQVGSQYMSMAGAKRAAEIIKSGRLGQIALVEGRIHRNAPTGAWYYPIPPDASPS
                     TVDFKRFLGPAPAREFDLRRFFQWRLFWDYSGGLPTDLFTHLITATHQLMGVQEPDTV
                     FAFADVYHWKNYREVPDQMTAMARYAEGFVLRLTSTAFNGHPGPLLTFYGSEGTMEYD
                     GDSFKLFHEPRSESFGYSTHSWPKATTARFRDLMNLNDRLVPLDAPDAADPVEYRSPG
                     EEDSTRAHMRNWIEAIRTGGKPIEDVRFGHHAALVGHMCNLSFKAGKPARWNKATRKV
                     EV"
     regulatory      4438..4643
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4718..7015
                     /locus_tag="PLT35_03570"
     CDS             4718..7015
                     /locus_tag="PLT35_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG28335.1"
                     /translation="MRPVSAFSLICSVLLLLTAGVSPASALSPAAPADGEPSLHGRVL
                     DPSGQPVAAARVSLRSASGTLRHTLSLADGSFAFDGLGPGAYDLTAAVDGFSAGIVPA
                     VVEPGAALRIDIPLRLAALTDTILVTASVIEVPRAEAPAGVVALTRRDLEDRQLDTVA
                     DALRLVPGLAVVPTGGLGSVTSVFPRGGESDFTLVLADGAKLNAFGGGIDFGHLTTFG
                     IDQIEVVRGPQSAVFGADAVGGVIHLRSKVGGPPAWNTLLEAGSRGTTRAAIGSTGSV
                     GALDWGVSAERVESDGWTGAAPNVALGRVTNDDYQARVFSAAAAWRAGSGTIVRASGR
                     FGRNERGYPGPFGADPIGAYGGIDAVSRGTNTFKAGSLELTREWGPATAVRLHASAGD
                     EESIFASPYGESASRTRRYAARGQFDRALSAALSASAGAAVEFERADSTFITGVDAQQ
                     VPVKRRVEGYFAELRWRSASRLFVTAGLRVEHIVRSALEADPWAWEPRPAMKTDGLVS
                     ANPRAAFSYYLRTSNESAGNWTRLHASAGTGIRPPAALEIAFTDNPGLRPERSRSAEA
                     GVEQALAGGLIVADATVFGNRYDDLIVAVGRALQDYSQFRSDNISNARAGGVEASLAL
                     RTKLGLEFRAAYSFVDTAILAVDRSAGTAPAPFAVGDWLVRRPRHQASADIVWRHALA
                     TLYVRAGGRSRVLDVEPNWGASGGLFHSPGFAVANAGAAIRLTRHADLVIRADNLFDK
                     RYESALGYPAPRRALSAGVRLAARR"
     gene            6999..7868
                     /locus_tag="PLT35_03575"
     CDS             6999..7868
                     /locus_tag="PLT35_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28336.1"
                     /translation="MLRAVDATYVHGRNLPAGAGGRRASPPAAAGAAGPVPPAPALPG
                     VQGVTMAIDRGRIVGILGPNGSGKTTLLRLLAGMLKPDAGTVTLEGRSLASIPRAALA
                     RSIAVVPQETHLAFDYSVLEIALMGRYPHLGTFQLEGPRDLAVAREALVATGTASLER
                     RRFSTLSGGEKQRVVIASALAQEAEILLLDEPTASLDVGSQFDVSALLARLNRDRGLT
                     IVVATHDLNFAAALCGSLVMLSAGRLIASGPTGETLTSLSIQALYGVVADVYPHRAAG
                     HLTVVPLRRIRRD"
     gene            7861..8865
                     /locus_tag="PLT35_03580"
     CDS             7861..8865
                     /locus_tag="PLT35_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG28337.1"
                     /translation="MTDVRRRLVAAVAAFGALAAAAVLAAPLAGSTPIRLDRVFDRSV
                     PFADNVDAQIFFIARLPRALAGALVGASLAAAGVVFQALLRNPLATPFTLGVSAGSAL
                     GAMLAITLRLHLSLWGVSAVTAASFAGAVCAVAIVYGMARARHSGFSTNVLLLAGVTL
                     NAFFSALILFVQYVADSAEALRTVRWLMGDLDVAGYRPILAALPLCALSFAAFAWLPR
                     ALNLLSLDADAAASRGIDVLRAQRVAFFSASMATGAAVSLGGPIGFIGIIVPHLVRLI
                     VGADHRVTLPASALFGAAFLVGADVAARTVMAPVELPVGIITALVGGPFFLWLLVRAR
                     "
     gene            8949..>9232
                     /locus_tag="PLT35_03585"
     CDS             8949..>9232
                     /locus_tag="PLT35_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28338.1"
                     /translation="MRAPARCVFATACLAAVAVLLQLPGGARGPQAPGRVPQRIVSIV
                     PSATEVLFAIGAGPRVAAVGSFERFPPEVERLPRVGALVEANFATLLESAR"
BASE COUNT         1260 a         3358 c         3213 g         1401 t
ORIGIN      
        1 gggaagcgtc gcggtaggga ctgggggctg ggcgctgggc gctgctccga cgcgcctctc
       61 gccgcgttgg tgctgtgcca gcgtgaccgt ccgacggacc agcgcctggc acccagtcgc
      121 cagcgcctga ggcacacacg atgacccata tgcgcccgat cagggagacg atcacgctcg
      181 aggcggcgcg cgagattatc gaccgcgtca tcaagcccat ggagcgcacc gaacgtgtcc
      241 gtggggacct ggccagcggc cgcgtcgtct cgcgcgccgt cgaggcgcag gccgacgtgc
      301 ctccgttcac gcgcgcggcg atggacggat acgccgtccg cgctgccgat acggtcggcg
      361 cgtcaacgca cgagccgcgc acgctcgcca gggtggaaac ggtctacacc gggcaggtgc
      421 caacgaaggt cgtcggccgg gggcagtgca ccgagatcgc caccggcgcc ccggtgccgg
      481 acggcgcgga cgcggtcgtg atggtcgagg agaccgacaa ggacgcgggc ggggcggttc
      541 gcgtgttcag cccggtctac cccggccaga acgtgacgaa gcagggcgcc gacatccgcg
      601 cgggccagct ggtgctggcc ccgggcgacg tcctgaaccc cagccgcatc ggcgccatcg
      661 cggcgctcgg cgtcatcgat gtcgaggtct acgcccggcc ccgcgtcgcg atcctctcga
      721 ccggcaacga ggtggccgag ccgggcgccc cgctcgcgcc cgggcacatc tacgacatca
      781 acaggttcac cctcagcgcg gtgcttcgcg accacggcgc cgtcccggtg ccctatccca
      841 cggccccgga cacgatcccg gaactcgaac gcgccatcga ggcctgctcc gccgaggacc
      901 tcatcctctt ctccggcggc agctcggtcg gcgagcgcga tctcatcctc gacgtcgttg
      961 agcgaatggg cgaggtgctc ttccacggca tcgcggtcaa gcccggcaag ccgacgctct
     1021 tcggcacggt cgccggcaag cccgtcttcg gcatgcccgg ctaccccacc tcctgcctgt
     1081 cgaacgccta catgctgctc gtcgcgcccg tccggcgcat ggcgcggctg cccgagtgga
     1141 cgcctcgcgt gatccgcctg ccgctcggcc agcgcatcgt ctcgacgacg gggcggcacc
     1201 agttctacac ggtgcgggtc gtggacgggc aggcgatgcc cgcgttcaag gcgtccggcg
     1261 acatcacgag catgtcgcag gccgacggct acatcgaaat cccggcgcag gtggacgtcg
     1321 tcgagaaggg cgaggtcgtc gccctcacgc tcttctgaga aggggtcggg tctaattatg
     1381 cagacggccg atctccgctg acgattctgt cgcacgcacc gggattcgtc cgaatggaga
     1441 gccgacatgc cgcgtgacca gagcacgaca ctcgaggctg gcgaccgcgc gccgctcttc
     1501 acgctcccca accagaaagg cgtgcccgtg tcgctggccg aagcgctggc gaaaggcccg
     1561 gtcgtcctcg gcttccaccg gggtacctgg tgaccgaact gccgtcgccg gttcggcgac
     1621 ctggcgcgga acgcagaaca ctaccgggcg ctcggcgcga cggtgatggc cgtcgtcgcg
     1681 cagcgctccg agaaggtgaa acgccacatc gaggatacgg ggctgcccct cgatctgctc
     1741 atcgatgaca cgcgcgaggt ggtgaaggcc tacggcgtgt ggcaccagat cggcttcgac
     1801 gcctggaata tctcgaggcc ggcgctgttc atcatcgatc gcggcgggat cgtccgttac
     1861 gcgttcgtgg cggattggca gacggagttc ccggagcacg aagtcatcgt cgcggagttg
     1921 gcgaaagcgg ggggttagaa ctcagaatcc ggaatccaga attcagaatc acgactcggt
     1981 gaccagaaac caggagcctg actctggaga gcggccgccg tccagcgtag cggtctcaag
     2041 gccgcaaggg gcgacgactc gtgtggcacg tgtcgccgcc cctgcagctg ttcgtcttcc
     2101 agattctgaa ttccggcttc tggattctgc ctcgagcgcg aagcgctatc gagtgtaccg
     2161 cttcgcgacc tcgctccagt tcaccacgct ccaccacgcg tccacgtagt cggcgcgccg
     2221 gttctggtac ttcaggtagt aggcgtgctc ccacacgtcg atgcccatca cggcctgccg
     2281 gccctccatg atcgggttgt cctggttcgg cgtgctctcg atcgtcagct tgccgccggt
     2341 gttgacgagc cacgcccagc cgctgccgaa ccgcccgagg gcggccttct tgaactcgcc
     2401 cttgaacgcc tcgaagctgc cgaaggccgc cttgatggca tcggccagcg ggcccgaagg
     2461 ctcgccgccg gccttcgggg ccatgatctg ccagaacatc gcgtggttca catggccgcc
     2521 gccgttgttg cgcaccgccg tgcggaccga ctcgggcacc ctggacaggt cgcggacgag
     2581 gtcgtcggcc gacaggctct gcagctccgg ggccttctcg agcgccgcgt tcaggttcga
     2641 cacgtacgtg gcgtggtgct tgccgtggtg gatctgcatc gtcatggtgt cgatgaacgg
     2701 ctcgagggcg tcgaatgcgt acggcagggg cggcagttcg tgggccatct cgtgatctcc
     2761 ttttcgccga agctcgctcg gagcggctgg acggttcgcc agacgcggat cgcggcaaat
     2821 ccgacctgcc gcgacctgtt taagagacgc agcccgggct ggtttggtga cacgcggcct
     2881 cggcagcctg gcccggcagg ttcgcgtcac ggcaccggcg gcgtcaggaa cccgcaacag
     2941 caggatccgc gacggcggtt tgacaccctt ttccctgccg cgtaggatgc gcgacgaagc
     3001 gggccggcgt ggttgtcccg cggggaggcg catgatgtcg ggcgacaact ccatgaaccg
     3061 caggcagttc cttcggaaga cggccctggc ggccgcggcg gcgtccgccg caagcgcgcc
     3121 gcgcaagacg gccgcccaga tcaacaacgc ggccggcgtt tcgctccggc aggtcaacct
     3181 cggcttcatc ggcatcggca tccgcggcac gcagctgatg gaagcagcct ccggcatcgc
     3241 cggcgtccag gtgcgggccg cggccgactg ctacaagggc cacctcgacc gcgcggcgga
     3301 actcatcacg ccgcccccgg ccgtcacggg cgactacacg gagatcctcg cgcgcccgga
     3361 catcgacgcc atcgtcatcg ccacgcccga ccactggcac ctcaggatcc tccaggaggc
     3421 cctcgccgcc gggaagcacg tgtacgtgga gaagccgatg acgcacgcgt gggaggaagg
     3481 cgaggccttc atctcggccg tcgagaagag cggcaaggtc gtccaggtcg gcagccaata
     3541 catgagcatg gccggcgcga agcgggcggc agaaatcatc aagagcggcc ggctcggcca
     3601 gatcgccctc gtcgagggca ggatccaccg gaacgcgccc acgggcgcct ggtactatcc
     3661 gattccgccg gatgcctccc cctcgacggt ggacttcaag cggttcctcg ggcccgcgcc
     3721 ggcgcgggag ttcgacctcc ggcgcttctt ccagtggcgg ctgttctggg actactcggg
     3781 cggcctgccc acggacctgt tcacgcacct gatcacggcg acgcaccagt tgatgggcgt
     3841 ccaggaaccc gacacggtct tcgccttcgc cgacgtctac cactggaaga actaccgcga
     3901 ggtgcccgat cagatgaccg cgatggccag gtacgcggag ggcttcgtcc ttcggctcac
     3961 gagcacggcc ttcaacggcc acccgggccc gctcctcacc ttctacggca gcgaagggac
     4021 gatggagtac gacggcgact cgttcaagct cttccacgag ccccgttcgg agagcttcgg
     4081 ctactcgacg cactcgtggc cgaaggcgac gacggcgagg ttcagggacc tgatgaacct
     4141 gaacgaccgc ctggttcccc tcgacgcgcc ggacgccgcc gacccggtcg agtaccgctc
     4201 gccaggcgag gaggactcca cgcgggcgca catgaggaac tggatcgaag ccatccggac
     4261 cggcgggaag cccatcgagg acgttcggtt cggccaccac gcggcgctcg tcggccacat
     4321 gtgcaacctg tcgttcaagg ccgggaagcc cgcgcggtgg aacaaggcga cgaggaaagt
     4381 cgaagtctga ggctccgggg actccggggt cagatctggc cccggttccc cggttggtgt
     4441 acactcaagc cagtcagccc ggtcggttga tcagggaagc tgggtgcaaa gcccacacgg
     4501 tcccgccact gtaagcgggg agcgcgcgcg attgcggcgg ccgcacgacg gccgcccgtc
     4561 acggtgccgg aaggcgctgg aagacgaaac gcgcgcgtga cgatccgcga gtcaggagac
     4621 ctgtcgatcc ggccgccaca ctcactcctc cgcagggccc cgcccaacgg gtcccgggcg
     4681 gaaccatcgg tgcgaggtca caccgagagg aggtcgcgtg cgcccggttt ccgcattctc
     4741 tctcatctgc tccgtccttc tccttcttac ggccggcgtg tcgccggcca gcgcgctgtc
     4801 cccggctgcg cccgcggacg gcgagccgtc cctccacgga cgcgtcctcg acccgtcggg
     4861 tcagcccgtc gccgccgcgc gggtcagcct gcgttcggcg tcgggcacgc tgcgccacac
     4921 cctcagcctg gcagacgggt cgttcgcctt cgacgggctc ggcccgggcg cgtacgacct
     4981 gacggcggct gttgacggct tctcggccgg catcgtcccc gcggtcgtcg agcctggcgc
     5041 ggcgctccgc atcgacatcc cgcttcgcct cgccgccctg acggacacga tcctggtcac
     5101 ggcatccgtc atcgaggtgc cgcgcgcgga agcgccggcg ggcgtggtgg ccctgacgcg
     5161 ccgcgatctc gaggaccggc aactggacac cgtcgccgac gcccttcgcc tcgtgcccgg
     5221 actggcggtc gtgccgacgg gcgggctcgg cagcgtgacg tctgtcttcc cgcgcggcgg
     5281 cgagtccgac ttcacgctcg tgctggcgga cggcgccaag ctgaacgcct tcggcggagg
     5341 aatcgacttc ggccatctga cgacgttcgg catcgaccag atcgaagtcg tccggggtcc
     5401 gcagagcgcg gtgttcggcg ccgacgcggt gggcggcgtg attcacctgc ggtcgaaggt
     5461 gggcggccct cctgcctgga acactctcct cgaggccgga tcgcgcggga cgacgcgcgc
     5521 cgctatcgga tcgacgggct ccgtgggcgc gctcgactgg ggcgtgagcg ccgagcgggt
     5581 cgagagcgac ggctggacgg gcgcggcccc gaacgtcgcg ctgggcaggg tgacgaacga
     5641 cgactaccag gcgagggtgt tctcggcagc cgcggcgtgg cgggccggct ccggcacgat
     5701 cgtccgcgcc tcggggcgct tcggcaggaa tgagcgaggc tacccggggc catttggcgc
     5761 ggatccgatc ggagcctacg gcggcatcga cgccgtctcg cgaggcacga acacgttcaa
     5821 ggccggctcg ctggagctca cccgcgagtg gggcccggca acggccgtgc ggcttcacgc
     5881 cagcgccggc gacgaggagt cgatcttcgc cagcccgtac ggcgagtccg cgtcgcggac
     5941 gcgccggtac gccgcccgcg gccagttcga tcgcgccctc tccgcggcgc tcagcgcatc
     6001 ggcaggcgcg gccgtcgagt tcgagcgcgc cgacagcacg ttcatcaccg gcgtggacgc
     6061 gcagcaggtt ccggtgaagc ggcgggtgga gggctacttc gccgaactgc gctggcgctc
     6121 ggcctcccgc ctgttcgtga cagccggcct gcgcgtcgag cacatcgtgc gcagcgcgct
     6181 cgaagcggac ccgtgggcgt gggagccgcg cccggccatg aagacggacg gcctcgtctc
     6241 ggccaacccc cgcgccgcat tcagctacta cctgcggacc tcgaacgaga gcgcgggcaa
     6301 ctggacgcgc ctccacgcca gcgccggcac gggcatcagg ccgccggccg ccctggagat
     6361 cgccttcacc gacaacccgg gcctcaggcc cgagcgcagc cggagcgccg aggccggcgt
     6421 cgagcaggcg ctggccggtg gcctgatcgt ggcggacgcc accgtgttcg gcaaccgcta
     6481 cgacgacctc atcgtggccg tcggccgcgc gctccaggac tacagccagt tccgcagcga
     6541 caacatctcg aacgcccggg cgggcggcgt cgaggcgtca ctggccctgc gcacgaagct
     6601 cggcctcgag ttccgcgcgg cctattcctt cgtggacacg gccatcctgg cggtggaccg
     6661 ctcggccggc acggcgccgg cgcccttcgc ggtcggcgac tggctcgtcc gccggccccg
     6721 ccaccaggcg agcgcggaca tcgtgtggcg ccacgccctc gccacgctgt acgtgcgcgc
     6781 gggcgggcgc tcgcgcgtcc tcgacgtcga gccgaactgg ggcgcgtcgg ggggcctctt
     6841 ccactcgccg ggcttcgcgg tggccaacgc cggcgccgcg atccgcctga cgcgccacgc
     6901 ggacctcgtc atccgcgccg acaacctgtt cgacaagcgc tacgagtcgg cactcggcta
     6961 ccccgcccct cgccgcgccc tctcggctgg agtgcgcctt gctgcgcgcc gttgacgcca
     7021 cctacgtcca cggccgaaac ctgcccgccg gggccggcgg ccgccgtgcg tcgccgccgg
     7081 cggcggctgg ggcggccggc cctgttccgc ctgcgcccgc cctgcctggc gtccagggcg
     7141 tcacaatggc catcgaccgc ggccgcatcg tcggcatcct cggccccaac gggtccggca
     7201 agacgacgct gctgcggctg ctggcgggga tgctgaagcc ggacgcggga acggtcacgc
     7261 tggaaggcag gagcctggcg tccatcccgc gcgccgcgct ggcgcgttcg atcgcggtcg
     7321 tgccgcagga gacgcatctc gccttcgact actcggtgct cgagatcgcc ctcatggggc
     7381 gctacccgca cctcggcacc tttcagctcg aagggccgcg tgatctggcc gtcgcgcggg
     7441 aggcgctggt tgcgacgggc acggcctcgc tcgagcgccg ccgcttctcc accctctccg
     7501 gcggcgaaaa gcagcgcgtc gtcatcgcga gcgccctggc gcaggaggcc gagatcctgc
     7561 tgctcgacga gccgaccgcg tcgctggacg tcggctcgca gttcgacgtc tcggcgctgc
     7621 tcgcgcggct caaccgcgat cgcggcctga ccatcgtcgt cgccacgcac gacctgaact
     7681 tcgccgccgc gctctgcggg tcgctcgtta tgctgtcggc cgggcgcctc atcgcgtcgg
     7741 gccccaccgg cgagacgctc acgagcctgt ccatccaggc gttgtacggc gtcgtggccg
     7801 acgtgtaccc gcaccgcgcc gcgggccacc tgacggtcgt gccgctccgg aggatccgcc
     7861 gtgactgacg tgcgccgccg gctcgtcgcg gcggttgccg ccttcggcgc gctggcggcg
     7921 gccgccgtcc tcgcggcgcc gctggccggc tccacgccca tccggctgga tcgcgtgttc
     7981 gaccgctcgg tcccgttcgc cgacaacgtg gacgcgcaga tcttcttcat cgcccgcctg
     8041 ccccgggcgc tcgccggcgc gctggtcggc gcgtcgctgg cagcggcggg cgtggtcttc
     8101 caggcgctgc tgcgcaaccc gctcgccact ccgttcacgc tgggcgtctc ggcgggatcc
     8161 gccctgggcg ccatgctggc gatcacgctg cgcctgcacc tgtcgctctg gggcgtgtcg
     8221 gccgtcaccg ccgcgagttt cgccggcgcc gtgtgcgcgg tggccatcgt ctacggcatg
     8281 gcccgggcgc ggcacagcgg gttctcgacc aacgtactgc tgctcgccgg cgtcacgctg
     8341 aacgcgttct tctcggcgct gatcctgttc gtgcaatacg tcgccgattc cgccgaggcg
     8401 ctgcggaccg tgcggtggct gatgggcgac ctcgacgtcg ccggctaccg gcccatcctg
     8461 gcggccctgc cgctttgcgc gctttccttc gccgccttcg cgtggctccc gcgggcgctg
     8521 aacctcctca gcctcgacgc cgacgccgcc gcctcgcgcg gcatcgacgt gctgcgcgcg
     8581 cagcgcgtgg cgttcttcag cgcgtcgatg gccaccggcg ccgccgtctc cctcggcggc
     8641 cccatcgggt tcatcggcat catcgtcccc cacctggttc gcctgatcgt cggcgcggac
     8701 caccgcgtga cccttccggc atccgctctg ttcggagcgg cgttcctcgt cggcgccgac
     8761 gtcgcggccc gcaccgtgat ggcgcccgtc gagctgcctg tcggcatcat caccgccctc
     8821 gtcggcgggc cgttcttcct gtggctgctc gtgagggcgc gctagtgaca gcgacagagg
     8881 gcaagggacc gggggttggg ggctggggac cgggggccgg gcgccgggcg cttggcgcga
     8941 ggggccgggt gcgcgcgccg gcgcggtgcg tcttcgccac ggcttgtctc gcggccgtcg
     9001 ccgtcctgct tcagcttccg ggtggagccc ggggccctca ggccccgggg cgggttcccc
     9061 agcgcatcgt ctcgatcgtt ccttccgcga ccgaagtgct gttcgccatc ggcgccgggc
     9121 cgcgcgtggc ggcggtgggc agcttcgaac gattcccgcc ggaggtcgag cggctcccgc
     9181 gcgtgggcgc gctggtggag gccaacttcg ccaccctgct ggagagcgcc cg
//
LOCUS       DAOSIF010000061         9149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7200_length_9149_cov_5.398834, whole genome shotgun sequence.
ACCESSION   DAOSIF010000061 DAOSIF010000000
VERSION     DAOSIF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7200_length_9149_cov_5.398834"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1454)
                     /locus_tag="PLT35_03590"
     CDS             complement(36..1454)
                     /locus_tag="PLT35_03590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG28339.1"
                     /translation="MTRDDRVAMAGTAALLAALVALALALSVFRPFNNDEIEHVHSAW
                     HVYGGARPYIDFYQHHHPLLWYALAPLLALTGQSASAMHAFRAVFFVLAMAVVWATYR
                     LGLECRLPRPVALLGAVLLLSMTSFTQVAIEVRPDVPQALFGVLSAIFLVRLGRTGAA
                     RDGARAGALAALAFLFLQKAIVVLAFFPVVLAWYVLTGQASWRAMCRAAAAFALAFAA
                     VCLPLVAYLAATGSLEPGVIATWRLAAHIPAGRSRWSFLSAAMLRSFLRNAAFWAIAA
                     ATSVSFVARRLKADVAVPAVLGLGTAAALVALNRVADRYLVAAMPFLAVAAAAWLSDA
                     LEARRARGPRALVVLLAVCLLPAISMVRSIGRSNRNQLARMQYVLDRSAPGERMFDPW
                     RDYNLFRPDVHYFWFHGGGAIAREFSRMTGGRVAAYDTCGLLAAAEPPFVSDRRSDLP
                     RCGLAGLYRPTPFDRFVERAGR"
     gene            1672..2718
                     /locus_tag="PLT35_03595"
     CDS             1672..2718
                     /locus_tag="PLT35_03595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28340.1"
                     /translation="MTTARTSEPPSAVGGPAGAGRVSVIVPVRGGGDAFRVCMARLSA
                     CQPPPGEIIVVIDGGREGAARRDEAAGARVIALDAPSGPAAARNAGARAACGNVLLFV
                     DSDVAVRPDLAGRVAAAFDADPDLAALMGSYDDEPADRRFLSQYRNLLHHYVHQTGRE
                     EASTFWCGCGAIRRDAFLESGGFDPGFSGAMIEDIEFGYRLRAAGRRIRLRKDLQVTH
                     LKRWTAWSMLRTDVFSRAVPWTRLVLRSRVMPDDLNLRRGARASVALAAALAASLAWA
                     AWQPWSLAAAAAAAAGILALNAGFYRFLWRKRGPRFAVAAVPWHWLYFACAGTGFLIG
                     AGLEAAAAIRAAWR"
     gene            complement(2725..4125)
                     /locus_tag="PLT35_03600"
     CDS             complement(2725..4125)
                     /locus_tag="PLT35_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG28341.1"
                     /translation="MPSSDYVVLGAGMAGLTIAREIARAGRSVTVVERGAQVGGLART
                     VRRDGFAWDLGGHRFHSNNPGVVRWLEGLLGGDLLRVERRSRIYLRGRFIDYPIRLTQ
                     AIRAFGLANAVRGAASYAAAAVVNHRERAVTFEEWVTRRFGRVLYETYFKPYTEKVWG
                     IRCHELSAEWAAQRIGVPSLLNTVRQAVSRPAVAPATAVREFRYPRSGFGAITDRLAG
                     EIDRAGHTVLTSTTLASLRFGDGGAVVGVAGEDGARRTIDCGRVISTVPLGALLGAFA
                     GDPDLARTAADARLDYRGIVLVLMAVDARRISPDTWTYFPESSVVFGRSHEPKHFSPV
                     MVPRENVTSLALEVFASPGESAWTSDDRDLIARAAGDLEGIGWLREESLLHAHVVRVP
                     FAYPVHDIGFADRLARVTGALGRYPRLSLLGRTGAFMYRNADGVIEDCLRLAAALGLR
                     QADGVQPLPADEGRWV"
     gene            4255..7668
                     /locus_tag="PLT35_03605"
     CDS             4255..7668
                     /locus_tag="PLT35_03605"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28342.1"
                     /translation="MTTAARHPFVSAVLTLVCLMGAMSVAQHPAPPSTQPTADDFNQF
                     TWRWIGPTTFSGRITALAVPHGQSTTYYVLAASGGLWKTEDAGIHFDPIFEKGGTLSM
                     GWLAIAPSDPNILYLGTGEPMHARSSTHGNGLWKSTDAGRTWTRIGLEKSHFIPKVEV
                     DAKNPDIVYAAAEGRLYDNEMDCERGLFKSTDGGRTWTNLGPMPDRGVSDFVLDPRDS
                     DVIITASYKTFRRAWTYIDRHQGNALYKSVDSGRTWKKLTAGLPQGVALGRTGLAIFE
                     KNPSIVYARLDEEVNLGFQEREGVAAFRAPAPGGAPGFAAGGAGAAPNFRPGSSFAAL
                     RTFKLDPLFVKASGVRFVPFPAEKEADFIAKLNEAVADQDFLPKHAVDMARFVPAARK
                     AFAGDEATQAALDELEKIATQAPVDAGSSQGRARSQIVNRLVLQTLYSGILGSFEPQK
                     RNGVVYRSDDQGETWTRMTQYALGVGSGGRFEEPEEAAPSEDAGLAVQAPAPAQAGQA
                     APAGRGSGPAASSMQVNQTEGGYYGRITIDPNDDTIVYCGDTNTTVSKDSGRTFAATG
                     WDGPGRTHVDHRAVWVDPLNSNHILSANDGGVSETWDGGKHFSQKNAIMAQQFYNVSV
                     DQERPYNVMGGTQDNGSWIGPSQTRNAYGIFPPDWRYLSGGDGFYVVRNWWNPEYLYT
                     ESQFGNSSRLNLNTGETSRLAAQLTPEQAAAGEPALRYQWNAPIVLSPHNPGIVFLAS
                     QYVWRSLNRGEPGSFERISPDLTKADQEKIALSRKTNLQWATVYTFAESPRKPGLYWA
                     GTDDGNVQVTVDGGVNWTNVTARFYDAAGRPKPGIKGDLIPYDRWVKRVVPSAFDENT
                     CYVGFSGYRTHSEDTTWLFVTKDLGRTWTNISGGMSNPIFDVEEDPDNANVLYLGTDN
                     GIWVTVDQGRTWTAFSTSQPTMVIRDLAIQKRDREMAIASYARGLWIADIAPIREFKP
                     EVFEKAAHLFEPQAAVRWNRYERRGDTLGEIVKAVNPPVGATIYYYLKADAQNVKITI
                     KDLEGNPVQEVTATARKGLQKIFWNLNRQAQPGAAPVGAGGRGAQAGAGGPGAAGPAP
                     QTPWAGFAAGRGGRGGGQVDPGAYKVTLTVDGHEVETKRLIVAPDPLFR"
     gene            7793..>9149
                     /locus_tag="PLT35_03610"
     CDS             7793..>9149
                     /locus_tag="PLT35_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG28343.1"
                     /translation="MRRRLQGLAVAAAVVAAALALSAMPSAQAPGDPIVDRIIELGTK
                     DNKTLVWNDYASNRFGGRETGSNSYTDAALWAVWQLKQFGLDARLEKAGELPVGFNRG
                     PWFGKMLKPTEKALRFGTPSFTAGTKGIQRGPVVILRADPFSIPGRAQAGRPVARENV
                     EKKRAAVQAALAEVAAKKAAFKGAWVLIAGASSGFARDGRRGSKLPDGSPEYLDAEMM
                     PPLTKALIDAGALGTIQSATPPSPLQPHQNSEPPINILDGFVASWDALPVLPDIKLLD
                     AQYKEIEALVEKGEPVVLEFDIRNHFKMGPIEYHNVVATLPGTAYPDEHIVIGGHFDA
                     FSSGTGGVDDGSGFAPGMEAIRLIAAAGAKPKRSITFIGFAAEEQGLVGSQAWLKQHP
                     ELHPKIVMMINRDGSPSAITGATVPETWYEDFKAITAPLASLNPRWPFVLARGVPRAH
                     AT"
BASE COUNT         1483 a         3198 c         3302 g         1166 t
ORIGIN      
        1 cgcccgaagc accggcgaag taaccgcgcc ccgcctcacc gcccggcgcg ttcgacgaac
       61 cggtcgaacg gcgtcgggcg gtacaggccc gccaggccgc agcggggcaa gtcgctccgc
      121 cgatccgaga cgaatggcgg ctctgcggct gcgaggagtc cgcaggtgtc gtaggccgcc
      181 acgcggccgc ccgtcatccg gctgaactcc ctcgcgatcg cccctccccc gtgaaaccag
      241 aagtagtgga cgtccggcct gaacaggttg tagtcgcgcc acgggtcgaa catgcgctcg
      301 ccgggcgcgg atcgatccag cacgtactgc atgcgcgcca gctgattccg gttcgatcgg
      361 ccgatggatc ggaccattga gatcgcgggc agcaggcaca cggcgagcag gacgacgagg
      421 gcgcgagggc cgcgggcgcg tctggcctcc agcgcgtcgg acagccaggc ggccgccgca
      481 accgccagga acggcatcgc cgcgacgagg taccggtccg ccacgcggtt gagcgcgacc
      541 agcgcggccg ccgtcccgag tcccaggacg gccggcacgg cgacgtcggc cttcagtctg
      601 cgcgccacga aggagacgct cgtggcggcc gcgatcgccc agaaggcggc gttcctgagg
      661 aagctccgaa gcatggcggc gctcaggaag ctccagcgcg agcgcccggc cgggatatgc
      721 gcggcgagcc gccaggtggc gatgacgccc ggctcgagcg atcccgtggc cgccaggtac
      781 gcgacgagcg gcaggcacac ggcggcgaag gccagggcga aggccgccgc ggcgcggcac
      841 atcgcgcgcc aggacgcctg tccggtgagg acgtaccagg cgaggacgac cgggaagaac
      901 gccagcacga cgatggcttt ctggaggaag aggaaggcca gggccgcaag cgcgccggcg
      961 cgcgcaccgt cgcgcgccgc gcctgttcgc ccgagtcgca cgaggaagat cgccgagagc
     1021 acgccgaaca gggcctgggg cacgtcgggc ctgacctcga tggcgacctg cgtgaacgag
     1081 gtcatcgaga gcagcagcac ggcgccgagc agggccacgg ggcggggcag ccggcactcg
     1141 aggccgagcc ggtacgtcgc ccacacgacg gccatcgcga ggacgaagaa cacggccctg
     1201 aacgcgtgca tcgccgacgc ggactggccg gtcagggcca ggagcggggc cagggcgtac
     1261 cacagcagcg ggtggtgatg ctggtagaag tcgatgtacg ggcgagcgcc gccgtacacg
     1321 tgccaggcgc tgtggacgtg ctcgatctcg tcgttgttga agggcctgaa gacggacagc
     1381 gccagcgcca gtgcgaccaa ggcggccagc aacgcggccg ttccggccat tgcgacgcgg
     1441 tcgtctcgtg tcatggctcg tcgaaccttc ggacgcgcgg ggattctacc ggagggacgg
     1501 cgcgcggaca acatcgcgtc agccaccgtg ttgaccggcc gtgcgcgcgg ctggctcgac
     1561 gggcatcgcg tctgccgccg tccggcgccg ccgcgcgcct cgtctcgcgc cttgcgccgg
     1621 cgtcagaggg gcggctaagc tggagcgtcc cctgcccgga gcccgccacg catgaccact
     1681 gcccgcacat ccgagccgcc ttctgccgtc ggcgggccgg ccggggccgg gcgcgtgtcg
     1741 gtcatcgtgc cggtgcgcgg cggcggcgac gcctttcgcg tctgcatggc ccggctgtcc
     1801 gcgtgccagc cgcctcccgg cgagatcatc gtcgtgatcg acggcggacg cgagggcgcg
     1861 gcccgccgcg acgaggcggc cggcgcgcgc gtcatcgcgc tcgacgcgcc gtccggcccc
     1921 gctgcggccc gcaacgccgg cgcgagggcc gcgtgcggga acgtcctcct gttcgtcgac
     1981 tcggacgtcg ccgtgcggcc cgatctggcc ggccgcgtcg ccgccgcgtt cgacgccgat
     2041 cccgatctcg ccgcgctcat gggctcgtac gacgacgagc cggctgaccg ccggttcctg
     2101 tcgcagtacc ggaacctcct gcaccactac gtccaccaga ccgggcgcga ggaggcgtcg
     2161 accttctggt gcggatgcgg ggcgatccgg cgcgatgcgt tcctggaatc ggggggcttc
     2221 gatccgggct tcagcggcgc gatgatcgag gacatcgagt tcggctaccg gcttcgggcg
     2281 gccgggcgcc ggatacgcct gcgcaaggac ctgcaggtca ctcacctgaa gcggtggacg
     2341 gcgtggtcga tgctgcggac cgacgtcttc tcgcgcgcgg tcccgtggac ccggctggtc
     2401 ctccgctcgc gcgtgatgcc cgacgacctg aacctgcgca ggggcgcgcg cgccagcgtc
     2461 gcgctggcgg ccgcgctggc cgcgtcgttg gcttgggcgg cctggcagcc ctggagcctc
     2521 gcggcggccg cggcggccgc ggccgggatc ctggcgctga acgcggggtt ctaccggttc
     2581 ctctggcgga agcgtgggcc gaggttcgcc gtcgcggctg tcccgtggca ctggctgtac
     2641 ttcgcctgcg cgggaaccgg cttcctcatc ggcgccggcc tggaggccgc cgccgccatc
     2701 cgggccgcgt ggaggtgagc cccgctacac ccagcggccc tcgtctgccg gcagcggctg
     2761 cacgccgtcg gcctgtcgaa gcccgagcgc cgcggcgagg cgcaagcagt cctcgatcac
     2821 gccgtcggcg ttccggtaca tgaacgcgcc ggtccggccg agcagcgaga gccgggggta
     2881 gcggccgagc gcccccgtga cgcgtgcgag gcgatcggcg aagccgatat cgtgcaccgg
     2941 gtaagcgaag ggcacgcgga cgacgtgcgc gtggagcagc gactcctctc gcagccatcc
     3001 gatgccttcg agatcgcccg cggcgcgcgc aatgaggtcc cggtcgtcgc tcgtccaggc
     3061 ggactcgcca ggcgaggcga acacctcgag cgcgagcgac gtgacgttct cgcgcggcac
     3121 catgaccggg ctgaagtgct tcggctcgtg gctgcggccg aacacgacac tcgattccgg
     3181 gaagtacgtc caggtgtccg gcgagatccg gcgggcgtcc acggccatga gcaccagcac
     3241 gatgccgcgg tagtcgagcc gcgcgtcggc ggccgtccgc gccaggtccg gatcgccggc
     3301 gaaggcgccg agcagcgcgc ccagcggcac cgtcgagatg acgcggccgc agtcgatcgt
     3361 ccgccgcgcg ccgtcttcgc ccgccacgcc gacaaccgct cccccgtcgc cgaaccggag
     3421 cgacgccagg gtcgtggacg tcaggacggt gtggcccgcc cgatcgatct cgccggcgag
     3481 ccggtccgtg atcgcgccga agccggaccg cgggtagcgg aactcgcgca ccgcggtggc
     3541 tggagccaca gccggccgac ttacggcctg ccgcaccgtg ttcaggaggc tgggcacgcc
     3601 gatccgctgg gccgcccatt ctgccgacag ctcgtggcac cggatgcccc agaccttctc
     3661 ggtgtagggc ttgaagtaag tctcgtagag gacccggccg aagcgccggg tgacccactc
     3721 ctcgaacgtg accgcccgtt cgcggtggtt cacgaccgcc gcggccgcgt aggacgccgc
     3781 gccgcgcacg gcgttggcca gcccgaacgc ccggatcgcc tgcgtcaggc ggatcggata
     3841 gtcgatgaac cgcccgcgca ggtagatccg gctgcgccgc tcgacgcgca gcaggtcgcc
     3901 tcccagcagg ccctcgagcc accggacgac accgggattg ttggaatgga agcgatggcc
     3961 gccgaggtcc caggcgaacc cgtcgcggcg aaccgtccgc gccagcccgc cgacctgcgc
     4021 gccgcgctcg acgacagtga ccgagcggcc ggcgcgcgcg atctcccgcg cgatggtcag
     4081 gcccgccatc ccggcgccga gcaccacgta gtccgatgag ggcatcctgt caaaggatat
     4141 catcgacgat cgcgcccagg cccgccggcg cgaggccgcc gcttccgcgg cggattcgac
     4201 tcgggtatgc tctccgggat cactgtcggg aagacgcttc cccgggaggc ccccgtgacc
     4261 acagcagcgc gacatccgtt cgtctcggcg gtgctgacgc tcgtgtgtct gatgggcgcg
     4321 atgagcgtcg cccagcatcc ggccccgccg tccacgcagc cgacggccga cgatttcaac
     4381 cagttcacct ggcggtggat cggcccgacg acgttctcgg gccggatcac cgcgcttgcg
     4441 gtcccgcacg gccagagcac gacgtactac gtgctggcgg cgtcgggcgg cttgtggaag
     4501 accgaggacg cgggcatcca cttcgatccg atcttcgaga agggcggcac gctcagcatg
     4561 ggctggctcg ccatcgcgcc gtcggacccg aacatcctct atctgggcac cggcgagccg
     4621 atgcacgcgc gctcgagcac ccacggcaac ggcctctgga agtccaccga cgccggcagg
     4681 acgtggacgc gaatcggcct cgagaagagc catttcatcc ccaaggtcga ggtggacgcg
     4741 aagaaccccg acatcgtgta cgccgccgcc gagggcaggc tctacgacaa cgagatggac
     4801 tgcgagcgcg gcctcttcaa gagcaccgac ggcgggagga cgtggacgaa cctcggcccg
     4861 atgccggacc gcggcgtctc cgacttcgtg ctcgatccgc gcgactccga tgtgatcatc
     4921 acggcgtcct acaagacctt ccggcgggcg tggacctaca tcgaccgcca ccagggcaac
     4981 gcgctgtaca agagcgtgga cagcggcagg acctggaaga agctgacggc ggggctcccg
     5041 cagggcgtgg cgctcgggcg cacgggcctc gcgatcttcg agaagaaccc gtccatcgtc
     5101 tacgcgcggc tggacgaaga agtcaacctc gggttccagg agcgcgaggg cgtcgccgcc
     5161 ttccgcgcgc ccgcacccgg aggggccccg ggattcgccg ccggcggcgc cggggccgcg
     5221 ccgaacttca ggccgggatc gtcgttcgcg gcgctcagga cgttcaagct cgacccgctc
     5281 ttcgtcaagg cgagcggcgt caggttcgtc cccttcccgg cggagaagga agccgacttc
     5341 atcgcgaagc tgaacgaggc ggtcgccgac caggacttcc tgccgaagca cgccgtcgac
     5401 atggcgaggt tcgttcccgc tgcccggaag gcgtttgccg gagacgaggc gacgcaggcc
     5461 gccctcgacg agctggagaa gatcgcgacg caggcgccgg tggacgccgg gtcctcgcaa
     5521 ggcagggccc gctcccagat cgtcaaccgc ctcgtgctcc agaccctgta ctcggggatc
     5581 ctcgggagct tcgagcccca gaagcgcaac ggcgtcgtct accgcagcga cgaccagggc
     5641 gagacctgga cgcgcatgac gcagtacgcc ctgggcgtag gcagcggcgg ccgcttcgag
     5701 gaacccgaag aagccgcccc gtccgaagac gccggactcg ccgtccaggc ccccgccccg
     5761 gcgcaggcgg gccaggcggc gccggccgga cgcgggagcg ggcctgcggc gagcagcatg
     5821 caggtgaacc agaccgaggg cggctactac ggccggatca ccatcgaccc gaacgacgac
     5881 acgatcgtgt actgcggcga caccaacacc accgtgtcga aggacagcgg caggaccttc
     5941 gcggcgacgg gatgggacgg ccccggcagg acccacgtgg atcaccgcgc ggtctgggtc
     6001 gacccgctca actcgaacca catcctgagc gccaacgacg gcggcgtgtc cgagacgtgg
     6061 gacggcggaa agcacttcag ccagaagaac gccatcatgg cgcagcagtt ctacaacgtc
     6121 tccgtcgatc aggagcggcc gtacaacgtc atgggcggca cgcaggacaa cgggtcgtgg
     6181 attgggccgt cgcagacgcg caacgcgtac ggcatcttcc cgcccgactg gcgctacctc
     6241 tccgggggcg acgggttcta cgtggtccgg aactggtgga accccgagta cctctacacc
     6301 gagagccagt tcggcaactc cagccggctg aacctgaaca ccggcgagac gagccggctg
     6361 gcggcccagc tcacgccgga gcaggcggcg gccggggagc cggccctccg ctaccagtgg
     6421 aacgcgccga tcgtgctctc gccgcacaac ccgggcatcg tcttcctcgc gagccagtac
     6481 gtgtggcgat cgctcaaccg gggcgagccg ggctcgttcg agcggatcag cccggatctc
     6541 acgaaggcgg accaggagaa gatcgcgctg tcccgcaaga cgaacctgca gtgggcgacg
     6601 gtgtacacct tcgccgagtc gccgcggaag cccggcctct actgggcggg caccgacgac
     6661 gggaacgtgc aggtgacggt ggacggcggg gtgaactgga cgaacgtcac ggcccggttc
     6721 tacgacgcgg cgggcaggcc gaaacccggg atcaagggcg acctgattcc gtacgaccgg
     6781 tgggtgaagc gcgtcgtgcc ctctgccttc gacgagaaca cgtgctacgt cgggttcagc
     6841 gggtaccgga cgcacagcga ggacacgacc tggctgttcg tcacgaagga cctcggcagg
     6901 acctggacga acatctcggg cggcatgagc aacccgatct tcgacgtcga ggaggacccg
     6961 gacaacgcga acgtgctcta cctcggaacg gacaacggga tctgggtgac cgtggaccag
     7021 ggcaggacct ggacggcgtt cagcacgtcg cagccgacga tggtcatccg cgacctcgcc
     7081 attcagaaac gcgaccgcga gatggccatc gcgagctacg cgcgggggct ctggatcgcc
     7141 gacatcgctc cgatccggga gttcaagccg gaggtcttcg agaaggccgc gcacctgttc
     7201 gagccgcagg cagcggtcag gtggaaccgc tacgagcggc gcggcgacac gctgggcgaa
     7261 atcgtcaagg ccgtcaaccc gccagtgggc gcgacgatct actactacct gaaggccgac
     7321 gcgcagaacg tgaagatcac gatcaaggac ctcgagggga acccggtcca ggaggtcacc
     7381 gcgacggcca ggaaggggct gcagaagatc ttctggaact tgaaccgcca ggcgcagccg
     7441 ggcgcggcgc ccgtcggggc ggggggccga ggcgcccagg ccggtgctgg cgggcccggc
     7501 gcggcgggcc cggcgcccca gacgccgtgg gccgggtttg ccgccggccg cggcggccga
     7561 ggaggcggcc aggtggatcc gggcgcgtac aaggtgacac tgacggtcga cggccacgag
     7621 gtcgagacca agcgcctgat tgtcgcccct gatccgttgt tcaggtgagc gagggggcct
     7681 ggctccgttc ggggggcccg gctgagggaa atcggcggca ggccgcgaga tgcgggggcc
     7741 tggccccttt ccgagaggga gcgggagcct ggccccttct ggtggaggaa ccatgagaag
     7801 acgactgcaa gggctggcgg tcgcggcggc cgtcgtggcc gcggcgctcg cgctctcggc
     7861 catgccgagc gcgcaggccc cgggagaccc gatcgtcgac aggatcatcg agctgggcac
     7921 gaaggacaac aagactctgg tctggaacga ttacgccagc aaccgcttcg gcgggcgcga
     7981 gacgggcagc aattcctaca ccgacgccgc gctgtgggcc gtctggcagc tgaagcagtt
     8041 cgggctggac gcgcggctcg agaaggccgg agaactgcca gtcggcttca accggggccc
     8101 gtggttcggc aagatgctga agcccacgga gaaggcgctc cgcttcggca cgccgagctt
     8161 cacggcgggc acgaagggca ttcagcgcgg accggtcgtc atcttgcggg ccgatccctt
     8221 ctcgatcccg gggcgcgccc aggccggtcg gccggtggcc cgggaaaacg tcgagaagaa
     8281 gcgcgcggcg gtgcaggcgg cgcttgccga agtcgcggcg aagaaggccg cgttcaaggg
     8341 cgcgtgggtg ctcatcgccg gggcctcgag cgggttcgcc cgcgacggcc ggcggggctc
     8401 gaagctgccg gacggctcgc ccgagtacct cgacgccgag atgatgcccc cgctcacgaa
     8461 ggccctgatc gacgcgggcg cgctcggcac gattcagtcg gccaccccgc cttcgccgct
     8521 ccagcctcac cagaactcgg agccgcccat caacatcctc gacggattcg tggcctcgtg
     8581 ggacgcgctc cccgtcctcc ccgacatcaa gctgctcgac gcgcagtaca aggaaatcga
     8641 ggccctcgtc gagaagggcg agccggtcgt cctcgagttc gacatccgga accacttcaa
     8701 gatggggccg atcgagtacc acaacgtggt ggcgacgctc ccgggcacgg cctaccccga
     8761 cgagcacatc gtcatcggcg gccacttcga cgccttcagc tcaggcacgg gcggcgtgga
     8821 cgacgggtcc gggttcgcgc cgggcatgga ggccatccgc ctgattgccg ccgcgggcgc
     8881 gaagccgaag cggtcgatca cgttcatcgg gttcgcggcg gaagagcagg gcctcgtcgg
     8941 gtcgcaggcg tggctgaagc agcacccgga gctccacccg aagatcgtga tgatgatcaa
     9001 ccgcgacggc agcccgagcg ccatcaccgg cgcgaccgtg ccggaaacgt ggtacgagga
     9061 cttcaaggcg atcacggcgc cgctggcctc gctcaatccg aggtggccct tcgtgctcgc
     9121 aaggggcgtc ccccgcgcgc acgctacga
//
LOCUS       DAOSIF010000062         9087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7247_length_9087_cov_4.122011, whole genome shotgun sequence.
ACCESSION   DAOSIF010000062 DAOSIF010000000
VERSION     DAOSIF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7247_length_9087_cov_4.122011"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLT35_03615"
     CDS             complement(<1..279)
                     /locus_tag="PLT35_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOG28344.1"
                     /translation="MARVYNWQLGRDMSYWYPEKRPQRQFAAVFDTNKCIACQTCTLA
                     CKTTWTSGKGQEYMLWNNVESKPYGSYPLAYDVRLLEQLDGQRWEGSRY"
     gene            complement(279..>2772)
                     /locus_tag="PLT35_03620"
     CDS             complement(279..>2772)
                     /locus_tag="PLT35_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG28345.1"
                     /translation="TEARLKGTKVVVIACEYSATACKGDEVIIVRPGTTPALALGLSH
                     VILRDELYDREYVTQWTDLPVLVRMDTLKNLRAQDVFGGPPAALTQTKVLGAGEVEAP
                     PIAQRDTIVSEKMRGEWGDYVWWDRRSQAPKPLTRDQVGRRSPVRDPLLEGAVEVALK
                     DGRTVTCRPVFDLLKTYAAHFDPATTESITWAPAAAVESLARHVAKEPGTTLFALGMG
                     PNQFFNSDNKDRATILLASLTGNVGRIGGNIGSYAGNYRTALFNGCAQYINENPFDIE
                     LDPEKPARPKQYWRAESAHYYNHEDHPLRVGKKLLTGSTHIPAPTKSLWFANANSILG
                     NVKWHYNTVLNVLPRIEMIAVHEWWWTASCEWADIVFGVDSWSELKHPDMTASVTNPF
                     LAVFPRTPLKRIFDTMGDIEVQALVGSKLAGLTGDRRFLDYWQFVREGRVDAYLQRIL
                     DFSTNTRGLRFADLHEKASRGIPALLNSRTSPKAVGFDQVADSKPWYTKTGRLEFYRE
                     EDEFIEAGENLPVHREPVDSTFYEPNVIVAPPHEAIRPASPESYGLSRRDLSCEARCG
                     RNVVMTWPEASRTKHPLRQDPAYRFIFHTPKYRHGSHTMPIDIDMIAVLFGPFGDLYR
                     HDRRSPFVTEGYVDINPSDARELGVDDGDYVWIDADPEDRPFRGWQRSPRDMEFARLL
                     CRARYYPGTPRGVTRMWFNMYTATPGSIEGQKTRPDGLAKNPRTGYQAMFRSGSHQSA
                     TRGWLKPTWMTDSLVHKTMFGQGMRQGFEPDIHCPTGAPRESFVKITRAEPGGIDRKG
                     LWRPAAMGIRPRYESDAMQKYLAGGFVAKEKP"
     assembly_gap    2773..2782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2783..3713)
                     /locus_tag="PLT35_03625"
     CDS             complement(<2783..3713)
                     /locus_tag="PLT35_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28346.1"
                     /translation="MSAQIPRRDFFGMVAAAGLGSLLASTVDAWGLDQVSNPLATYPD
                     RGWERVYRDLWKYDSTFTFTCAPNDTHNCILNAYVRNGVVVRIGPTMRYGEAADLAGN
                     TTTHRWDPRVCQKGLALTRRFYGDRRVSGCMVRAGFRKWFEAGFPRGADGLPPPTYFR
                     RGRDEWVRVTHDEGAAIAAAALKNIAETYSGAAGAARLRAQHYDEATIEAVRGAGVQT
                     MKFRGGMPLLGMTRVFGMYRLANSMALLDAHVRKVGADQALGAKGFDNYSWHTDLPPG
                     HPMVTGQQTVEFDLSAVEHCKTLVVWGMNWVTTK"
     gene            complement(3710..5365)
                     /locus_tag="PLT35_03630"
     CDS             complement(3710..5365)
                     /locus_tag="PLT35_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOG28347.1"
                     /translation="MNKNKYLLLVSSAAVLLLLIGAAVQETFLQEWRRLQARARTDEG
                     PIPIQLRQVVNDSLRAADRCVSCHVAMGPGEQGVRGEPVLAAHKPVVHDPAEFGCTIC
                     HAGQGQATTRSEAHGDVRFWPEPMIPARFSYAGCGRCHATLGVPEHRQLQAAAAAFER
                     LDCLSCHRVDGRGGTLRPDGGGMEGPDLSRVGLAGYDPGWYETHLNKARTASAGPWRG
                     AVGPSSDEDRRLLALFLSTRVASPMLVEAKSVFHSAGCLGCHKVSGVGGDDGMDLSRA
                     GDKDPGQVSFHGVPGQPTIANWQLAHFRSPASIVAGSLMPPVAASDRELDLLAMYVLS
                     LRRRDLPEVYVPPDRARSVKLGEREFAGDGATLFGAFCSGCHGQDGLGRRLPGVLSFP
                     SIASLDFLAIASDGLIVSTIERGRTGRRMAAWLKPGGLRPAEVRAVAGYLRSLGGVPP
                     EEDPRPARWVTADAAAGRKLFDGVCAGCHGPAGEGGEGPALNSIVLRDSATDTFLVKT
                     IGRGRRGTVMPGFLSATPAYPALASADIEDVVAYLRVLQGGGS"
     gene            complement(5371..6927)
                     /locus_tag="PLT35_03635"
     CDS             complement(5371..6927)
                     /locus_tag="PLT35_03635"
                     /inference="COORDINATES: protein motif:HMM:NF025016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOG28348.1"
                     /translation="MRASPGGRAALAEFRANLKAAPRRLYESAFRSGAPTTDRSRSTF
                     VFGNVFLHLHAVRTHRWSLRWTTTLGLGVMSASAFLITLVTGVMLMFYYKPSPDLAYQ
                     SIKDIHFVVPTGRLIRNIHRWAANVMVVAVILHMARAFYTSAYRSPREYNWLIGLLLL
                     VVTLGLSFTGYLLPWDQLAYWAITIGANIAQSPRELTDALGVTGLFDPGGFQRLLLLG
                     SDVVGQEALIRFYLLHVMILPLALAALAAVHFWRIRKDGGLTRPADADARLAREPAAD
                     TYPVFTEAPLRTYHLAAVVRGRTPAVNDGPEHTLPSMPHLFYAELGVLMLTLLVCVAL
                     ALVSDAPLKELANPSVPENPAKAPWYFLGLQEMVAFSAFMGGMGIPLIVLLGLALIPF
                     LDRETSATGEWWGGPGGWPLVKRSAFVGLAAPVAIEAFAIRFGWLREWFPAIPQLVVT
                     AVNPATLLTAAYAAYSVWCIRRHQSARAGALALFTCFLGGFVVLTAIGTYFRGPNWVF
                     YWSPADWPTH"
     gene            complement(6927..7409)
                     /locus_tag="PLT35_03640"
     CDS             complement(6927..7409)
                     /locus_tag="PLT35_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOG28349.1"
                     /translation="MTVTPKPSRSRLDAEPVSRRDWLGLTSLGVTAGALLFGLVGLLR
                     LPKAAVLSSPSKKFRIALPEGLPAGEAFVPPGRAVAVFRDARGVHAISITCTHLGCLV
                     KPTAEGFECPCHGSRFAADGSVTKGPAPRPLPWLKVSVSGSEVTIDEGTAVASGTKEV
                     "
     gene            complement(7406..>9087)
                     /locus_tag="PLT35_03645"
     CDS             complement(7406..>9087)
                     /locus_tag="PLT35_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG28350.1"
                     /translation="VDDDGRFVAEDRPIAIRALKRFVCDRFGPDARAPADVLASVEAA
                     PPTLASGVEEIAALIRSRRDGRLVPAEGQRVAIVGGGPAGLSAAHDLALLGLRPVVFE
                     AEPVPAGMLAVGVPAYRLPRDLIEREVGVIRALGVEIRCGVAVGRDVSFASLRRGFDA
                     VIIAVGAKSSRALGLPGERGPRVYGGVDLLRSVALGEAIDIGREVVVIGGGNVAYDVA
                     RTVVRQIAFDAARTAARLPGTAGVRLVSLEGLEDMPADTVEIAEGDEEGVARMNGWGP
                     VEIERDHEGGVRAVHLRRCLRVYDDARRFAPVFDDGDRLRLECDTVLLSVGQAPVLSF
                     LADGGADIEMARPGWPRVDPVSLETTAPGVFVAGDLAHGTRLIIDAVASGKAAARAVY
                     THLTGRPLESDLLELHLAEDGYRRERGYESIRRVRVPLADPADRLRHPEALVELGYDA
                     ALAAREASRCLDCGITPVFDGTRCVLCGGCADVCPTRCLKLVSLDAIAETPEIAAAVA
                     GALGDGIDPAEHSGILKDEDRCIRCAACVMRCPTDAIKMDRVSYCASWRSA"
BASE COUNT         1336 a         3258 c         3200 g         1283 t
ORIGIN      
        1 gtagcgcgag ccttcccacc gctggccgtc cagctgctcg agcagcctga cgtcgtaggc
       61 cagcgggtag gagccgtagg gcttgctctc gacgttgttc cagagcatgt actcctggcc
      121 cttgcccgac gtccaggtcg tcttgcaggc cagcgtgcag gtctggcagg cgatgcactt
      181 gttcgtgtcg aacaccgcag cgaactgccg ctgcgggcgc ttctcgggat accagtagga
      241 catgtcgcgc ccgagctgcc agttgtagac gcgtgccatc agggcttctc cttcgcgacg
      301 aatccgccgg cgaggtactt ctgcatcgcg tccgattcgt accgcgggcg gatgcccatc
      361 gcggccgggc gccagagccc cttccggtcg atccccccgg gttccgcccg cgtgatcttc
      421 acgaacgact cgcgcggcgc gcccgttgga cagtggatgt ccggctcgaa gccctggcgc
      481 atcccctggc cgaacatggt cttgtgcacg agcgagtcgg tcatccaggt cggcttcagc
      541 cagccgcgcg tggcggactg gtgcgatccc gagcggaaca tggcctggta gccggtgcgc
      601 gggttcttcg cgagcccgtc cggacgggtc ttctgcccct cgatcgagcc cggcgtcgcc
      661 gtgtacatgt tgaaccacat ccgcgtcacg ccgcggggcg tgcccgggta gtagcgcgcc
      721 cggcagagca gccgggcgaa ctccatgtcc cgcgggctgc gctgccaccc ccggaacggg
      781 cgatcctccg ggtcggcgtc gatccagacg tagtccccgt cgtcgacgcc cagttccctg
      841 gcgtcggacg ggttgatgtc cacgtagccc tccgtcacga acgggctgcg ccggtcgtgg
      901 cggtagagat cgccgaacgg cccgaacagg acggcgatca tgtcgatgtc gatgggcatc
      961 gtgtgcgagc cgtggcggta cttcggcgtg tggaagatga acctgtacgc cgggtcctgc
     1021 cggagggggt gcttcgtccg gctcgcctcg ggccaggtca tcacgacgtt gcggccgcag
     1081 cgcgcctcgc acgacaggtc ccggcggctg aggccgtacg actccggact ggcgggccgg
     1141 atggcctcgt gcgggggcgc gacgatcacg ttcggctcgt agaaggtcga gtccaccggc
     1201 tcgcggtgca cgggcaggtt ctcgccggcc tcgatgaact cgtcctcctc gcggtagaac
     1261 tcgagccgcc cggtcttcgt gtaccagggc ttcgagtcgg ccacctggtc gaacccgacc
     1321 gccttcggcg acgtgcggct gttcagcagc gccgggatgc cgcggctggc cttctcgtgg
     1381 agatcggcga agcggaggcc cctggtgttc gtcgagaagt cgaggatgcg ctgcaggtac
     1441 gcgtccacgc ggccctcgcg cacgaactgc cagtaatcga ggaaccgccg gtcgcccgtg
     1501 agcccggcca gcttcgaccc gacgagcgcc tgcacctcga tgtcgcccat cgtgtcgaag
     1561 atccgcttga gcggcgtgcg gggaaagacg gccaggaacg ggttcgtgac cgacgccgtc
     1621 atgtccgggt gtttcagctc gctccacgag tcgacgccga acacgatgtc cgcccactcg
     1681 cacgaagccg tccaccacca ctcgtgcacc gcgatcatct cgatgcgagg cagcacgttg
     1741 aggacggtgt tgtagtgcca cttgacgttg ccgaggatcg agttggcgtt cgcgaaccag
     1801 agcgacttcg tgggggccgg gatgtgggtg gagcccgtca gcagcttctt ccccacgcgc
     1861 agcgggtgat cctcgtggtt gtagtagtgg gcggactcgg cgcgccagta ctgcttcggc
     1921 cgcgccggtt tctccgggtc gagctcgatg tcgaacgggt tctcgttgat gtactgcgcg
     1981 cagccgttga agagcgcggt ccggtagttg ccggcgtacg acccgatgtt ccctccgatg
     2041 cggccgacgt tcccggtcag cgacgccagc aggatggtgg cgcggtcctt gttgtcgctg
     2101 ttgaagaact ggttcggccc cattccgagc gcgaacagcg tggtcccagg ctccttcgcc
     2161 acgtgccgcg cgagcgactc gacggccgcg gcgggggccc aggtgatcga ctcggtcgtt
     2221 gccgggtcga agtgcgcggc gtacgtcttc agcaggtcga acacgggacg gcaggtgacc
     2281 gtccggccgt ccttgagagc cacctcgacg gcgccctcca gcagggggtc ccgcaccggg
     2341 gagcgccgcc ccacctggtc gcgggtcagg ggcttcggcg cctgcgatcg gcggtcccac
     2401 cagacgtagt cgccccactc cccccgcatc ttctcgctca cgatcgtgtc gcgctgggcg
     2461 atgggcggcg cttccacctc gcccgcgccc aggaccttcg tctgcgtgag cgcggccggc
     2521 ggcccgccga acacgtcctg cgcccggagg ttcttcagcg tgtccatcct caccagcacc
     2581 ggcaggtccg tccactgcgt gacgtactcg cggtcgtaga gctcgtcccg caggatcacg
     2641 tgcgacaatc cgagcgccag cgcgggcgtc gtgcccggcc gcacgatgat gacctcgtcg
     2701 cccttgcagg cggtggccga gtactcgcac gcgatgacca cgactttcgt gcccttcagg
     2761 cgcgcctcgg tcnnnnnnnn nntcttcgtc gtgacccagt tcatgcccca gacgacgagc
     2821 gtcttgcagt gctcgaccgc gctcaggtcg aactcgaccg tctgctggcc ggtgaccatc
     2881 ggatggcccg gcggcaggtc ggtgtgccag gagtagttgt cgaacccctt ggcgccgagc
     2941 gcctggtccg cgccgacctt gcggacgtgc gcgtcgagca gggccatcga gttggcgagg
     3001 cggtacatgc cgaagacgcg ggtcatcccg agcagcggca tgccgccccg gaacttcatc
     3061 gtctgcacgc cggcgccccg cacggcctcg atggtggcct cgtcgtagtg ctgggcccgg
     3121 aggcgcgccg cgcccgccgc gccgctgtag gtctcggcga tgttcttcag ggccgcggcc
     3181 gcaatggccg cgccctcgtc gtgcgtcacg cggacccact cgtcccgccc gcgccggaag
     3241 tacgtcggcg gcgggagccc gtcggccccg cgggggaagc ccgcctcgaa ccacttcctg
     3301 aaccccgcgc gcaccatgca gccgctcacc cgccgatcgc cgtagaagcg gcgcgtcagc
     3361 gcgagcccct tctggcacac gcgagggtcc caccggtgcg tcgtcgtgtt gccggcgagg
     3421 tccgcggcct ccccgtacct catcgtcggt ccgatcctca ccaccacgcc gttccggacg
     3481 taggcgttca ggatgcagtt gtgggtgtcg ttgggcgcgc aggtgaaggt gaacgtcgag
     3541 tcgtacttcc acaggtcgcg gtacacccgc tcccagcctc gatccgggta cgtggcgagc
     3601 gggttcgaca cctggtcgag cccccatgcg tccaccgtgg acgcgaggag gctgccgagc
     3661 ccggccgcgg cgaccatccc gaagaagtcc ctgcgcggga tctgcgcgct catgatccgc
     3721 ctccctgcag cacccggaga taggccacga cgtcctcgat gtccgcgctc gcgagcgcag
     3781 gatacgccgg cgtggccgag aggaagcccg gcatgaccgt gccgcgccgc ccgcggccga
     3841 tggtcttcac caggaacgtg tccgtcgcgc tgtcgcgcag cacgatgctg ttcagggcgg
     3901 ggccctcgcc gccctcgcct gccggcccgt ggcagccggc gcagacgccg tcgaacagct
     3961 tccgcccggc ggcggcgtcg gccgtgaccc agcgggccgg acgggggtcc tcctcgggcg
     4021 gaacgccccc cagcgaccgc aggtagcctg caacggcccg cacctcggcc gggcgcaggc
     4081 cgccgggctt gagccaggcg gccatccgcc ggccggtcct cccccgctcg atcgtggaga
     4141 caatcagccc gtctgaagcg atggccagga agtccaggct ggcgatggag gggaacgaca
     4201 gcacgcccgg caggcggcgg cccagcccgt cctgcccgtg gcagcccgag cagaaggcgc
     4261 cgaacagcgt cgcgccgtcc ccagcgaact cgcgctcgcc cagcttgacc gatcgcgcgc
     4321 ggtccggggg cacgtagacc tcgggcaggt cgcggcgccg cagcgacagg acgtacatgg
     4381 ccagcaggtc gagttcccga tccgacgccg cgacgggcgg catcagcgat ccggccacga
     4441 tgctcgccgg cgatcggaaa tgggccagct gccagttcgc gatcgtcggt tgcccgggca
     4501 cgccgtggaa gctcacctgc cccgggtcct tgtcccccgc cctggacaag tccatcccat
     4561 cgtcgccgcc gacgccgctg actttgtggc agccgaggca gccggccgag tgaaagacgc
     4621 tcttcgcctc gacgagcatc ggcgacgcga cgcgcgtcga caggaacagc gccaggagcc
     4681 gccggtcctc gtcgctcgaa gggccgacgg cgccacgcca cgggcccgcg gaggctgtcc
     4741 gcgccttgtt caggtgcgtc tcgtaccagc ccgggtcgta tccggcgagg cccacgcggc
     4801 tcaggtccgg cccttccatg cccccgccgt cgggcctgag ggtgccgccg cggccgtcca
     4861 cgcgatggca ggacaggcag tcgagccgct cgaaggcggc ggctgccgcc tgcagttgcc
     4921 gatgctcagg gacccccagc gtggcgtggc agcggccgca cccggcgtag ctgaagcggg
     4981 cggggatcat cggctcgggc cagaagcgca cgtcgccgtg ggcctcggac cgtgtcgtgg
     5041 cctgtccctg gcccgcgtgg cagatcgtgc agccgaattc ggccggatcg tggaccaccg
     5101 gcttgtgggc ggcgagcacc ggttcgcccc tgacgccctg ctcgccgggg cccatcgcga
     5161 cgtggcacga cacgcagcgg tccgccgcgc ggaggctgtc gttcacgacc tgccgaagct
     5221 ggattggaat cgggccctcg tcggtgcgcg cccgtgcctg aagccgccgc cattcctgca
     5281 ggaaggtctc ctggacggcg gccccgatca gcaggagcag caccgcggcg ctcgaaacga
     5341 gcaggaggta cttgttcttg ttcatgggcg tcagtgcgtc ggccagtcgg cgggcgacca
     5401 gtagaacacc cagttcggcc cccggaagta ggtgccgatg gcggtcagca ccacgaagcc
     5461 gcccaggaag caggtgaaca gcgcgagggc cccggcccgg gcggactggt gccgccgaat
     5521 gcaccagacc gagtacgccg cgtacgccgc ggtgagcagc gtggcgggat tgacggcggt
     5581 cacgaccagc tgcggaatgg cgggaaacca ctcgcggagc cacccgaacc ggatggcgaa
     5641 ggcctcgatg gccaccgggg ccgccagtcc cacaaacgcc gagcgcttca cgagcggcca
     5701 gccgccgggt ccgccccacc attcgccggt cgcgctggtt tcgcggtcga ggaatggaat
     5761 cagggcgagg ccgagcagca cgatcagcgg gatccccatg ccgcccatga aggccgagaa
     5821 ggcgaccatt tcctggagcc ccaggaagta ccacggcgcc ttggcggggt tctcgggcac
     5881 gctcgggttg gccagctcct tgagcggcgc gtccgagacc agcgccaggg cgacgcagac
     5941 gagcagcgtg agcatcagca ccccgagctc ggcgtagaag aggtgcggca tcgagggcag
     6001 cgtgtgctcc ggcccgtcgt tcacggcggg cgtccttccg cgcacgacgg ccgccaggtg
     6061 gtacgtccgg aggggcgcct ccgtgaacac cgggtacgtg tcagcggcgg gctctcgtgc
     6121 gagccgcgcg tccgcgtcgg ccggccgcgt gagcccgccg tctttgcgga tgcgccagaa
     6181 gtggacggct gccagcgcgg ccagcgcgag cggcaggatc atcacgtgca gcaggtagaa
     6241 cctgatgagc gcctcctgcc cgaccacgtc ggagccgagc aggagcagcc gctgaaagcc
     6301 gcccggatcg aagaggccgg tcacgcccag ggcgtcggtc agctcgcggg gcgactgcgc
     6361 gatgttcgcg ccgatcgtga tggcccagta cgccagctga tcccagggca gcaggtaccc
     6421 cgtgaacgac agcccgagcg tcacgaccag cagcaggagg ccaatcagcc agttgtactc
     6481 gcgggggctc cggtacgcgg acgtgtagaa ggcccgggcc atgtggagga tcaccgccac
     6541 gaccatgacg ttcgcggccc accggtggat gttccggatc agcctccccg tcggcacgac
     6601 gaagtggatg tccttgatgg actgatacgc gaggtccggc gacggcttgt agtagaacat
     6661 cagcatcacg ccggtcacga gcgtgatgag gaacgccgac gcgctcatca cgcccaggcc
     6721 gagcgtcgtc gtccaccgca ggctccagcg gtgcgtgcgc acggcgtgga ggtgcaggaa
     6781 cacgttgccg aacacgaagg tggagcgcga gcgatccgtc gtcggcgcgc ccgaacggaa
     6841 cgccgactcg tagagccggc gcggggccgc cttgaggttg gcgcggaact cggcgagcgc
     6901 cgcgcggccg cctggcgaag cgcgcatcac acctccttcg tgcccgaggc gaccgccgtg
     6961 ccttcgtcga tcgtcacctc gctgccgctg acgctgacct tgagccaggg cagcgggcgc
     7021 ggggccggcc ctttcgtgac cgatccgtcg gccgcgaacc gcgacccgtg gcacgggcac
     7081 tcgaacccct cggctgtagg cttgacgagg cagccgagat gggtgcaggt gatggaaatc
     7141 gcgtggacgc cccgcgcgtc gcggaacacc gcgaccgcgc gccccggagg cacgaacgcc
     7201 tcgcccgcgg gcaggccctc gggcagcgcg atgcggaact tcttggacgg ggacgacaac
     7261 accgcggcct tcggcagccg cagcaggccg accaggccga acagcagcgc gccggccgtg
     7321 acgcccagcg acgtcaggcc cagccagtcg cggcgcgaga caggctcggc atcgagcctc
     7381 gaccgggagg gcttcggagt gaccgtcatg cggacctcca cgaggcacag taggacacgc
     7441 ggtccatctt gatcgcgtcc gtcgggcacc gcatgacgca cgcggcgcag cggatgcagc
     7501 ggtcctcgtc tttgagaatg cccgaatgct ccgccgggtc gatcccgtcg ccgagcgcgc
     7561 ccgcgacggc ggccgcgatc tccggcgtct ccgcgattgc atcgagcgac acgagcttca
     7621 ggcaacgcgt cgggcacacg tcggcgcacc cgccgcacag cacgcagcgc gtgccgtcga
     7681 acaccggcgt gatgccgcag tccaggcagc gcgacgcttc gcgcgcggcg agcgccgcat
     7741 cgtagccgag ctcgaccagc gcctcgggat gccggaggcg gtccgccggg tccgccaggg
     7801 gcacccgcac gcggcggatg gactcgtagc cgcgctcgcg ccggtagccg tcctcggcca
     7861 ggtgcagctc gagcagatcg ctctcgaggg gccggcccgt gaggtgcgtg tacacggccc
     7921 gcgcggcggc cttcccggac gccaccgcgt cgatgatgag gcgcgtgccg tgggcgaggt
     7981 cgcccgccac gaacaccccc ggcgccgtgg tctcgaggct cacgggatcg acgcggggcc
     8041 agccgggccg cgccatctcg atgtccgcgc cgccgtccgc caggaaggag agcaccggcg
     8101 cctggcccac cgacagcagc acggtgtcgc actcgaggcg gaggcgatcg ccgtcgtcga
     8161 acaccggggc gaaccggcgt gcgtcgtcgt agacgcgcag gcagcgcctc agatgcacgg
     8221 cccggactcc gccctcgtgg tctcgttcga tctccaccgg gccccacccg ttcatccggg
     8281 cgacgccctc ctcatcgcct tcggcgatct ccaccgtgtc ggcgggcatg tcctcgaggc
     8341 cctcgagcga caccagccgg acgccggccg tgcccggcag gcgcgccgcc gtgcgggccg
     8401 cgtcgaaggc aatctgccgc acgacggtgc gggccacgtc gtaggccacg ttgccgccgc
     8461 cgatcaccac gacctcccgg ccgatgtcga tggcctcgcc gagcgccacc gatcgcagga
     8521 gatcgacgcc gccgtacacg cggggcccgc gctcgccggg caggcccagg gcgcgcgacg
     8581 acttggcgcc gacggcgatg atcaccgcat cgaagccgcg gcggaggctc gcgaaggaga
     8641 cgtcgcgccc gaccgccacg ccgcagcgga tctcgacgcc aagcgcacgg atgacgccga
     8701 cttcccgctc gatcagatcg cgggggagcc ggtaggcggg aaccccgaca gccagcatgc
     8761 cggccggaac gggctccgct tcgaacacga cggggcgcaa gcccagtagc gcgagatcgt
     8821 gggccgccga caggcccgcc ggcccccctc cgacgatcgc gacgcgctgg ccctccgcgg
     8881 ggacgagccg cccgtcgcgc cgggatcgaa tcagcgcggc aatctcctcc acgccggacg
     8941 ccagcgtggg cggggccgcc tcgaccgacg cgaggacgtc ggcgggcgcc cgggcgtcgg
     9001 gcccgaaccg gtcgcagacg aacctcttca aggcgcggat ggcaatcggg cgatcctcgg
     9061 cgacgaaccg gccgtcgtcg tcgacgc
//
LOCUS       DAOSIF010000063         9080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7255_length_9080_cov_4.154903, whole genome shotgun sequence.
ACCESSION   DAOSIF010000063 DAOSIF010000000
VERSION     DAOSIF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7255_length_9080_cov_4.154903"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..72)
                     /locus_tag="PLT35_03650"
     tRNA            complement(1..72)
                     /locus_tag="PLT35_03650"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Ala,seq:ggc)
     gene            complement(175..399)
                     /locus_tag="PLT35_03655"
     CDS             complement(175..399)
                     /locus_tag="PLT35_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28351.1"
                     /translation="MPTPIQRFEQQIGGRLYVIEASPVHANRWRAQIARRHGVPSALM
                     PFYGATADEAASQLVRWLALAHQRAAGTAS"
     gene            684..2108
                     /locus_tag="PLT35_03660"
     CDS             684..2108
                     /locus_tag="PLT35_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28352.1"
                     /translation="MRRGDHDGPRWAARTALVRACLAALLAAAIGDAAHDASAANVPS
                     YRIFLSDGTALVSFGEFARANGSVVFTMPIGAPANPDVLRVVTLPDTAIDWERTDRYA
                     DAVRHRHYASTRGEEDYAALTGAVARALGDMAFAPDAAAKLAIAADIRRQLLEWPATH
                     LGYRSGDVRELTAIVEEAISDIWAGSGGRTFDLSLVATIEPPSEPLLPEPTVKDTIDL
                     ASAAAGLTEARIERLSLQEQILAVLDRRRQGLPRDWYRTTRKAITSDLRREAELDERY
                     EALLARSRRSAREAAARADVAALERLAAEVQRQDARLGYRRPEDMRALLAAIAAQSER
                     ARQARAAREQWQARKSLFGAYRRRVGSSFGKFDDVATDIAAVREMSGVKTDRLEKTAR
                     RVSAIEVALLPLNPPEQLRPSHDLLVSSARLMKEAIRLWRIAAASGEMNTARNASAAA
                     AGALLLLDTARGRIEEYFRPPAAP"
     gene            2105..5047
                     /locus_tag="PLT35_03665"
     CDS             2105..5047
                     /locus_tag="PLT35_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG28353.1"
                     /translation="MIAPRSTRLVRAPDAAAFRRVLVGLALSGTLAAIRRRALVLPSL
                     AAADAFRRSLEEAALADGLPGAVAIPHLVTRDGWMAALQDAMPGAGRRASGLEREVLL
                     RASAETAGAAGAPPPFTLRPGLVAEMLAFYDAVRRHGRSVDDLERVAVGELERAADSD
                     RGAARMLAQTRFLVASFRDYESRLAAAGAADEHTLRRALLEAPGSPFDHVVVAVGDRA
                     GEPAGLWPSDYDLLARVPGLSRLDIVATAAALGAGLLDRLRRWLPGLDETDAPLDPVR
                     PPPPELLAPPGEASPPYWRARDREDELADVARTVKRASRTDPAEALGRTAVVFKRPLP
                     YVYLSRRVFASAGVPYQVFDALPLAAEPFAAAVDLAFDAAESGFSRAPLTALLRSPLL
                     QFSAGGRRVGAADAALLDRKLSEARYLAAPEDLPALAASWISPAGLHRAALAAAAVAE
                     ELAPLAREEPPTVHLDALRRFLDAHHRPPGAAHETRDRQLRVRAAVLSALARLRDAHA
                     RLDDRPRPFEETAALLRRWIEAQTFAPREGSAGVQLVDAEAARFGDFDTLNLVGLTEQ
                     EWPEGRPRSIFYPASMLADLGWPADADGRAAERAAFDDLLRSARRTVRVSTITLEEDA
                     IVEPSPFLDDLARCGLRVRRAPREPLPRVLAGEALVLDPPQPPSAGGPAAAWLALRLA
                     RTPADDARFHGSADPVMRATHRVSAIDRYLACPFAYFAEHVLRLAEEPEDEEALAPRA
                     QGTLVHAVLEAFYREWQARGCGAVTPENLPEARGLFASVALAHLASLPPGDAALERAR
                     LLGSPAAEGCGDIALSAEAERDRGVAVVERLLEHALDGDTPLGSGPASRTIRLSAKAD
                     RIDLFADGTFRVIDYKLSRAPALRHVVQLPAYAAAARERLAGRHGRSWRASDAAYLSF
                     GRSGHYEPIAAGQERLDAALAAGEARLLGAVAQIEQGAFPVAPVEPHRCRYCPFAGVC
                     RKEFAGDE"
     gene            5037..8465
                     /locus_tag="PLT35_03670"
     CDS             5037..8465
                     /locus_tag="PLT35_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG28354.1"
                     /translation="MTSDERRPVGRGPSRPGGGGEAARDEAARQAAVDPSRNVVLEAS
                     AGTGKTRVLVERYLNLLRAGVEPKNILAVTFTRKAASEMRQRIVAALRTDADASPEGA
                     ARWRGLRDRLGEIAISTIDAFCLSLLREFPLEADLDPGFGIADDTDVPRLIEESLDDA
                     LRVCRARARTDAGVALLFAQLGESRVRLGIAALLERRAMAGSVLGRLAALGPRGLSVE
                     EACRRGAERIAAALCAVPGGLERFIDAGPVGHPRYAVLAAELRDLARGAAAGAAPAAA
                     PERIKGLADRLQAHFFTKAGTARRTSTEYRADHFADPRGRAAYWALVNAAAPAVGDAL
                     RGLRRDLNAIQSRAVWQVFSIANERYRRTLEARGVLDFSELLARATALLGNMDEFARS
                     RYLLEARYHHVLVDEFQDTSRAQWELVSLLVRAWGEGLGLAAEAPLPPSIFIVGDRKQ
                     SIYRFRDADVRVLDEAAAAIEQLRPEGSPVRSISRSFRSAPPLLAFVNDLFAEIAGPA
                     GRPEAFRFGESDAFPVDAAGSHWPPGPALGLVAAASVRRSADAVAAEIARLAASGQVR
                     DRQSGELRPIRPADVGILFRSRASHREFERALEAWGIPSYVYRGLGFFDADEVKDTVA
                     LLRYLADPASDLRAAAFLRSRLVRLSDSAIQRLAPSIAQAAAGRLATPAAVLDPEDAR
                     VFARMRSSVDAWLDLADRVPPAELLDRVLAESAYEYELRGPRLAAARENLKKLRGLVR
                     RIQNRGYVTLERLAGVFDRLSAGEESNAVVDAIDAVNLMTVHAAKGLEFPVVFVVNLG
                     RGSGGPRAPIRIAADAPPEDAVAVGDYEAEFDERDAAHERAETTRLLYVAATRGRDRV
                     YLVSVCEDGRFRPRAGSLGEVLPPSFQAVLGEAASVQNDGTAVRWQAASGAAHVFRVC
                     VPPDAPPAGPPKPFAQAVAADTAAGAGPDGGADFGALEDEGGRQRLAVTAIGSSRPAS
                     WKEVDGQEGAGRPPRGAAEAVLGRVVHRMFQRAARGDANDGELASLARALVREDEQPA
                     VPDMEAFAARAAAVFARLWATPALRSLLDGAECLYEVPVSFVGPAPGAGAAQTVLRGV
                     IDCLAVRPDGSVVVVDFKTGARRRSDRRQLNAYVDAARLLFPGAPVEGLLVYAV"
     gene            complement(8462..8632)
                     /locus_tag="PLT35_03675"
     CDS             complement(8462..8632)
                     /locus_tag="PLT35_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28355.1"
                     /translation="MSAAEFEVRSEKSGGHWVAWIVRTPDGKPDRSVRIVGRTREEAE
                     ARARDWAEGRVR"
     gene            8727..8948
                     /locus_tag="PLT35_03680"
     CDS             8727..8948
                     /locus_tag="PLT35_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28356.1"
                     /translation="MAVACPSCGNARSFQVKTLQMHVIQVDDSRVNIAEENRPAVFEV
                     LCDECEAAVPFDEFDESTRREILLTLGAR"
BASE COUNT         1102 a         3407 c         3336 g         1235 t
ORIGIN      
        1 ggaccagacg ggaatcgaac ccgtgacctc gtgaatgcca ttcacgcgcg ctcccatctg
       61 cgctactggc ccacgtcaca cgcggacatc cgattgtagc gcgaggcccg agccggggtc
      121 aaagcggcgg gtgccgcgcc gggacgcgcg ccgaaagcct gtccggaacg gcggctacga
      181 ggcggtcccg gcagcccgct gatgggccag cgccagccac cgcacaagct gggaggccgc
      241 ctcgtccgcc gtggcgccgt agaaaggcat cagggccgat ggcacgccgt ggcggcgcgc
      301 gatctgggcg cgccagcgat tcgcgtggac cggcgacgcc tcgatgacgt acaggcgccc
      361 gccgatctgc tgctcgaacc gctgaatggg agtaggcatg agcgccagcg cccgcggcca
      421 acaggcagcc gcgacgcaca tggcattcta cgcaacgccc cggagcgatc aagcgaaggc
      481 atgcgcgaag gtcgcgtcaa catccgccag acggcgcgga gggcgctgag gccgcgggtt
      541 gcgcgtgcgg ttgtcggtgc gtcaacacgt cttgtacgcg ccgatccaca cgttttccac
      601 acgcgcgcgg gcgcgcagga cgcgccgcgc gcgcttgacg ctccctacgc cggacactag
      661 catgcttgga caggacatcc gcgatgcgcc gaggcgatca cgacggtccg cgatgggcgg
      721 cgcgcacggc gctggtgcgt gcgtgcctgg ccgcgctcct ggccgcggcg atcggcgacg
      781 ccgcacacga cgcctccgcc gccaatgtcc cttcctaccg catcttcctc tccgacggca
      841 ccgcactcgt cagtttcggc gaattcgcgc gggccaacgg gagcgtcgtc ttcacgatgc
      901 cgatcggcgc gccggccaac ccggacgtcc tgcgcgtggt gacgctgccc gacaccgcca
      961 tcgactggga gcgcaccgac cgctatgccg acgccgtccg ccatcgccac tacgcctcga
     1021 cccggggcga agaagactac gcggccctga ccggggccgt ggcccgcgcc ctgggcgaca
     1081 tggcgttcgc gccggacgcg gccgccaagc tcgccatcgc cgccgacatc cgccgccagc
     1141 tgctcgagtg gcccgccacg cacctgggct accgctccgg cgacgttcgc gaactgacgg
     1201 ccatcgtgga ggaagccatc tccgacatct gggcgggttc gggcgggcgc accttcgacc
     1261 tcagcctcgt ggcgacgatc gagccgccgt ccgagccgct gctgcccgag ccgacggtga
     1321 aggacacgat cgatctggcg tcggccgcgg ccgggctgac ggaagcgcgg atcgagcggc
     1381 tctcgctcca ggagcagatc ctcgccgtac tagatcggcg caggcagggc ctgccccgcg
     1441 actggtaccg gaccacgcgc aaggccatca cctcggatct gaggcgcgag gcggagctgg
     1501 acgagcgtta cgaggctctc ctggcccgct cgcgtcgctc ggcccgggag gcggcggctc
     1561 gcgccgacgt cgcggctctc gagcgtttgg cggccgaggt gcagcggcag gacgcgcggc
     1621 tcggctaccg gcgccctgag gacatgcggg cgctcctggc cgcgattgcc gcgcagtcgg
     1681 agcgggcgcg gcaggcgcgc gccgcgcggg agcaatggca ggcgcgcaag tcgctgttcg
     1741 gcgcgtacag gcgccgcgtc ggctcgagct tcgggaagtt cgacgacgtg gcgaccgaca
     1801 tcgcggcggt tcgggagatg tccggcgtca agacggatcg cctggagaag acggccaggc
     1861 gcgtgtcggc gatcgaggtg gcgctgctgc cgctcaaccc gccggagcag ctgcggccgt
     1921 cgcacgacct cctggtttcg tcggcgcgcc tgatgaagga agcgattcgg ctctggcgca
     1981 tcgccgccgc gtcgggcgag atgaacaccg cccgcaacgc gtcggcggcg gcggccggcg
     2041 cgctgctgct gctcgacacg gcccggggcc gcatcgagga gtacttccgc cctccagccg
     2101 cgccgtgatc gcgccgcgct cgaccaggct cgttcgcgcg cccgacgccg ccgcgttccg
     2161 gcgcgtcctc gtcggcctgg ccctgtccgg cacgctcgcg gcgatccgcc ggcgcgccct
     2221 cgtcctgccc tccctagcgg ccgccgatgc cttcaggcgg tcccttgaag aggcggccct
     2281 cgccgacggc cttcccggcg cagtggccat cccgcacctg gtgacgcgcg acggctggat
     2341 ggcggccttg caggacgcga tgccgggggc agggcgccgc gcctcaggcc tcgagcgcga
     2401 agtgctgctt cgggcctcgg ccgagaccgc gggggcggcc ggcgcgccgc cgccgttcac
     2461 gcttcgcccc ggtctcgtgg ccgagatgct cgcattctac gacgccgtgc ggcggcacgg
     2521 ccggagcgtg gacgatctcg agcgggtcgc cgtcggcgaa ctcgagcgcg ccgccgactc
     2581 ggatcgcggc gcggcgcgga tgctggcgca gacgcgtttc ctggtcgcgt cgttccgcga
     2641 ctacgagtcc cgcctggccg ccgcgggagc ggccgacgag cacacgctcc gccgcgcgct
     2701 gctcgaggcg ccgggctcgc ccttcgatca cgtggtcgtg gcggttggcg accgcgccgg
     2761 cgagccggcc ggcctgtggc cctcggatta cgacttgctg gcgcgcgtcc cgggcctgtc
     2821 gcgcctcgac atcgtggcca ccgccgccgc gctcggagcc ggcctgctcg atcgcctgcg
     2881 gcgctggctc ccagggcttg acgagacgga tgcgccgctc gatccggtgc gcccgccgcc
     2941 gcccgaactc ctggccccgc ctggcgaggc gtcgccgccc tactggcgtg cgcgcgaccg
     3001 cgaggacgaa ctggcggacg tcgcgcggac ggtcaagcgg gcgagccgga ctgaccccgc
     3061 cgaggcgctc ggccgtacgg cggtggtctt caaacggccc ctgccgtacg tctacctctc
     3121 gcgtcgggtg ttcgcgtcgg cgggcgtgcc gtaccaggtg ttcgacgcgc tcccgctggc
     3181 cgccgagccg tttgccgccg ccgtcgatct cgccttcgac gccgccgagt cgggcttctc
     3241 gcgcgctccg ctcaccgccc tgctgcgctc gccgctcctg cagttctccg cagggggccg
     3301 gcgcgtcggg gcggcggatg ccgcgctgct cgatcggaag ctgagcgagg cgcgctacct
     3361 tgccgctccc gaagacctgc cggcgctggc ggcgtcgtgg atctcgcccg cgggcctcca
     3421 ccgcgccgcc ctcgcggcgg cggccgtggc ggaagagctg gcgccgctcg cgcgcgagga
     3481 gccgccgacg gttcatctcg acgcgctgcg gcgctttctc gacgcgcacc accggccgcc
     3541 cggcgccgcg cacgagacgc gcgaccggca gctcagggtc cgcgcggccg tcttgtcggc
     3601 gctggctcgg ctccgggacg cgcacgcgcg cctggacgac cggccgaggc ccttcgagga
     3661 gacggcggcg ctccttcgcc gctggatcga agcgcagacg ttcgcgccgc gcgagggctc
     3721 cgccggggtg cagctcgtcg acgcggaggc ggcacggttc ggcgacttcg acacgctgaa
     3781 cctggtcggg ctgaccgagc aggagtggcc cgagggccgg ccgcgcagca tcttctaccc
     3841 ggcgtccatg ctcgcggacc tcggctggcc cgcagacgcc gacggccggg ccgccgagcg
     3901 cgccgcgttc gacgacctgc tccgctcggc ccggcgcacg gtgcgggtct ccacgatcac
     3961 gctggaagag gacgcgatcg tcgagccgtc gcccttcctc gacgatctcg cgcggtgcgg
     4021 cctgcgcgtc cggcgcgcgc cgcgcgagcc cctccctcgc gtgcttgccg gcgaggcgct
     4081 cgtcctcgat ccgccgcagc cgccatccgc cggcggcccg gctgcggcgt ggctggcgct
     4141 gcgcctcgcc cgcacgccgg ccgacgacgc caggttccac ggctcggccg atcccgtcat
     4201 gcgcgcgacc caccgggtga gcgccatcga tcgctacctg gcgtgcccgt tcgcctactt
     4261 cgcggagcac gttctgcgcc tggcggaaga gcccgaggac gaggaggccc tggcgccgag
     4321 ggcgcagggc acgctcgtgc acgcggtgct cgaggctttc taccgcgaat ggcaggcccg
     4381 cggctgcggc gccgtgacgc ccgagaacct gccggaagcc cgcggcctct tcgcctccgt
     4441 cgccctcgcg cacctggcct cgctgccgcc cggcgatgcc gcgctcgagc gcgcgcggct
     4501 cctcggctcg cctgccgccg aggggtgcgg ggacatcgcc ctgtcggccg aagccgaacg
     4561 cgatcgcggc gtggccgtcg tcgagcggct cctcgagcac gcgctcgacg gggacacgcc
     4621 cctcggctcg ggaccggcat ccaggaccat ccgcctgtcg gcgaaggcgg accgcatcga
     4681 cctgttcgcc gacgggacgt tcagggtcat cgactacaag ctgtcgcgcg cgccggcgct
     4741 caggcacgtc gtgcagttgc ccgcctacgc cgccgctgcg cgcgagcgcc tcgccgggcg
     4801 gcacggccga tcgtggcgcg cgtcggacgc ggcctacctg tcgttcggcc ggagcggcca
     4861 ctacgagccg atcgccgcgg gccaggagcg gctcgacgcc gcgctggccg cgggcgaagc
     4921 gcggctcctc ggcgccgtcg cgcagatcga gcagggggcg ttcccggtcg cgcccgtcga
     4981 gccacaccgg tgccggtact gcccgttcgc cggcgtctgc cggaaggagt tcgccggtga
     5041 cgagtgacga acgccggccg gtcggacggg ggccgagccg gccggggggc ggcggcgaag
     5101 ccgcccgcga cgaagccgcg cggcaggcgg cggtggatcc gtcgcgcaac gtcgtgctcg
     5161 aggcgtccgc cggcacgggc aagacccggg tgctcgtgga gcggtacctc aaccttctgc
     5221 gcgccggcgt ggagcccaag aacattctcg cggtcacgtt cacgcgcaag gccgcctccg
     5281 agatgcggca gcgcatcgtg gccgctcttc gcacggacgc ggacgcgtcg cctgagggcg
     5341 ccgcccgctg gcgcggcctc cgggaccggt tgggcgagat cgccatcagc acgatcgacg
     5401 ccttctgcct gtcgctgctc cgcgagttcc ccctcgaagc ggatctcgat cccggcttcg
     5461 gcatcgccga cgacacggac gtgccgaggc tgatcgagga gtcgctcgac gacgcgctgc
     5521 gggtctgccg cgcgcgcgcg cgcaccgacg ccggcgtggc gctgctcttc gcgcagctcg
     5581 gcgaatcgcg cgtgcgcctc gggatcgcgg cgcttctcga gcgccgcgcc atggccggga
     5641 gcgtcctcgg acgcctcgcc gcgctggggc cccgcggcct cagcgtcgag gaagcgtgcc
     5701 gtcggggggc cgagagaatc gccgccgcgc tctgcgccgt gcccgggggc ctcgagcggt
     5761 tcatcgacgc ggggcccgtc ggccaccccc gctacgccgt gctcgcggcg gaactgaggg
     5821 atctggcgcg cggcgcggcg gcgggcgcgg cgccggcggc cgcaccggag cgcatcaagg
     5881 gcctcgcgga ccggctgcag gcgcacttct tcacgaaggc gggcacggcg aggcgaacct
     5941 cgacggagta ccgcgccgat cacttcgccg acccgcgggg ccgcgcggcg tactgggccc
     6001 ttgtcaacgc cgcggcgcca gccgtgggcg atgcgctccg cggactgcgg cgcgacctga
     6061 acgcgatcca gtcgcgcgcg gtctggcagg tgttctcgat cgcgaacgag cgctaccggc
     6121 ggaccctcga ggcccgcggc gtgctcgact tcagcgagct gctcgcgcgc gcgaccgcgc
     6181 tcctcggcaa catggacgag ttcgcccgga gccggtacct gctcgaggcc cgctaccatc
     6241 acgtgctcgt ggacgagttc caggacacgt cccgcgcgca gtgggagctg gtgtcgctgc
     6301 tcgtccgcgc gtggggcgaa gggctcgggc tggccgcgga ggccccgctc ccgccctcga
     6361 tcttcatcgt cggcgaccgg aagcagtcca tctaccggtt ccgcgacgcg gacgtccgcg
     6421 tcctcgacga agccgccgcc gccatcgagc agctgcggcc ggaagggtcc cctgtccgca
     6481 gcatctcgcg gagcttccgg tccgcgccgc cgctgctggc cttcgtcaac gacctgttcg
     6541 cggagatcgc cgggccggcg ggccggcccg aggccttcag gttcggcgag agcgacgcgt
     6601 ttcccgtgga cgcagccggc tcgcactggc cgcccgggcc ggcgctgggc ctcgtggcgg
     6661 ccgcgagcgt gcgccggtcc gccgacgccg tggccgcgga gattgcgcgc ctggccgcga
     6721 gcggccaggt gcgcgaccgc cagtcgggcg agctgcgccc catcaggccc gccgacgtcg
     6781 gcattctctt ccgctcccgg gccagccacc gcgagttcga gcgggcgctc gaggcgtggg
     6841 gcatcccgtc ctacgtgtac cgcgggctgg ggttcttcga cgccgacgag gtgaaggaca
     6901 ccgtggcgct gctgcggtat ctcgccgacc cggcgtccga cctgcgcgcg gcggccttcc
     6961 tgcgctcgcg gctcgtccgc ctgtccgaca gcgccatcca gcgcctggcg ccgtcaatcg
     7021 cccaagcagc ggcggggcgt ctggcgacac ccgccgcggt gctcgatccc gaggacgccc
     7081 gggtcttcgc ccggatgcgc tcgagcgtgg acgcatggct cgacctcgcc gatcgcgtgc
     7141 cgccggccga gctgctggat cgcgtgctcg cggagtcggc gtacgagtac gaactgcgag
     7201 gcccgcgcct ggccgccgcg cgcgagaacc tgaagaagct gcggggcctt gtccgccgga
     7261 tccagaaccg gggctacgtg acgctcgagc gcctcgcggg ggtcttcgac cgcctgtccg
     7321 ccggggagga gtctaatgcc gtcgtcgatg ccatcgacgc cgtgaacctc atgaccgtgc
     7381 acgccgcgaa gggcctggag ttccccgtgg tcttcgtcgt gaacctcggg cggggatcgg
     7441 gcggcccgcg cgccccaatc cggatcgccg ccgacgcgcc cccggaggac gccgtggcgg
     7501 tgggcgacta cgaagccgag ttcgacgagc gcgacgcggc gcacgagcgc gcggagacga
     7561 cgcggctgct ctacgtcgcg gcgacccggg gccgcgaccg cgtctacctc gtgtctgtct
     7621 gcgaggacgg ccggttccgc ccgagggccg gcagcctggg cgaggtgctg ccgccttcgt
     7681 tccaggcggt cctcggggaa gcggcctccg tgcagaacga cggaacggcc gtccggtggc
     7741 aggccgcttc cggcgccgcg cacgtgttcc gggtctgcgt tccgccggac gcgccgccgg
     7801 cgggcccgcc gaagccgttc gcacaggccg tggccgccga cacggccgcc ggcgccggcc
     7861 cggacggcgg agcggacttc ggcgcactcg aggacgaggg cggccggcag cgcctcgcgg
     7921 tgacggcgat cggcagttcc agacccgcgt cctggaagga agttgacggc caggaaggcg
     7981 ccggccgccc gcctcgcggg gccgccgagg cggttctggg gcgcgtggtc catcgcatgt
     8041 tccagcgggc cgcacgcggt gacgcgaacg acggtgagct ggcgagcctg gcgcgtgcgc
     8101 tcgtccggga agacgagcag ccggcggtgc cggacatgga ggccttcgcg gcccgcgcgg
     8161 ctgccgtgtt cgcccgcctg tgggcgacgc cggccctgcg gtccctgctg gacggcgccg
     8221 agtgcctcta cgaggttccc gtctcgttcg tcgggccagc gccgggcgcg ggcgcggcgc
     8281 agacggtgct gcgcggcgtg atcgactgcc tcgccgtgcg gccggacggc agcgtcgtcg
     8341 tggtggattt caagaccggg gccaggcgca ggtccgaccg ccggcagttg aacgcgtatg
     8401 tggacgcggc gcgcctgctg tttcccggcg caccggtcga agggcttctg gtctacgcgg
     8461 tctagcggac cctgccctcg gcccagtccc gggcacgcgc ctcggcttct tcccgcgtcc
     8521 ggccgacaat ccgcaccgac cggtcgggct tgccgtccgg ggtccgaacg atccaggcca
     8581 cccagtggcc gccgctcttc tcagatcgga cttcgaactc cgcagcgctc atgattcagc
     8641 ttctccaaca cgcccttata ccgtacaatc gaacgatggg cacgtgccgg agagccggcc
     8701 cgtgcctccc ggagaaggag tcagcaatgg cagttgcgtg cccgagttgc ggcaacgcgc
     8761 ggagttttca ggtcaagacc ctgcagatgc acgtcattca ggtggatgac agccgcgtga
     8821 acatcgcgga agagaaccgg ccggccgtgt tcgaagtcct gtgcgacgaa tgcgaggccg
     8881 ccgtcccctt cgacgagttc gacgagagca cccgccggga aatcctgctg acgctcggcg
     8941 cccgctagcg cccgggcgtt cccgccgccg ttcctcgcca gccggcattg tcccagggtc
     9001 gggctccgga ggcctccagg cggccggccc ggccgcccgt cacctgcttt cggccggttc
     9061 gatggcgtcc tcttcgaatt
//
LOCUS       DAOSIF010000064         9024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7306_length_9024_cov_4.710001, whole genome shotgun sequence.
ACCESSION   DAOSIF010000064 DAOSIF010000000
VERSION     DAOSIF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7306_length_9024_cov_4.710001"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLT35_03685"
     CDS             complement(<1..533)
                     /locus_tag="PLT35_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28357.1"
                     /translation="MKREAVATLAFAALLAALAFSSSPREVGDASEYVRMAERLAAGR
                     LPGAGESRHFIAYPALAAPLVAALGALGSGPLRAFTILNGLLLAAAFRVCSARLHWAW
                     CAMVFAGPILWWIDKPHTEPFTFALLAVAFSLAESAPWWSLAAIGAAATQNPGNAALL
                     AVIAAAAAIGRPLLRAKL"
     gene            968..2344
                     /locus_tag="PLT35_03690"
     CDS             968..2344
                     /locus_tag="PLT35_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HOG28358.1"
                     /translation="MIDTSRILCIGAGYVGGPTMAVVAAKCPHIRVDVVDINEARIAA
                     WNSAELPIYEPGLDEVVRAARGRNLFFSTDIAGGIRDADIIFVSVNTPTKTFGQGAGY
                     AADLQHWEKTARQILAHATRDKIVVEKSTLPVRTAQAMERILDGNPAGVRFDVVSNPE
                     FLAEGSAIADLERPDRVLIGGHDTPAGRAARRIIADIYATWVPRERIIESNLWSAELS
                     KLTANAFLAQRISSINSISALCEVTGADVDEVAYAIGTDSRVGPRFLKASVGFGGSCF
                     KKDILNLVYICRSHGLGEVADYWESVVAINEWQQSRFVRNMLASMFNTVAGKRIALFG
                     FAFKADTGDTRESAAIGVARQLLAERAVVVITDPKALGSVREELPEEGDRIECEIDPY
                     RAARGAHAIAIMTEWRIYRDLDYARIFASMEKPAFVFDGRNILDHKRLHEIGFNVYAL
                     GKAPLRHV"
     gene            2541..4064
                     /locus_tag="PLT35_03695"
     CDS             2541..4064
                     /locus_tag="PLT35_03695"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOG28359.1"
                     /translation="MAIVLDGRSLTADVLVRIARFGEPVELAPAALERIASCRAMLER
                     KLAAREVMYGTNTGIGEFSERVLTDEQVQAFQRYLVYNHAAGIGAPAPVEHVRGAMAG
                     RINVHAHGNSGCRPEITLTLVEMLNKGVTPVVCQKGSVGACGDLAPMAQLALLLMGEG
                     EAFYRGERLPGAEAMRRAGIAVPGLEARDGLASINGSNLLTAMSALHVWDMQRWLRQA
                     EIAAAMTIEALLGNLKPYNARLHELRGFPGAVQSAANIMKVLEGSDLTTGRMKTKVQD
                     AYSMRSTPQVIGAARDAIAWARAQVETELNGVGDNPIFIPEQDLTLTGANFQGSPVAL
                     PMDMAGAAVTMVSVLSERRLNRLTNPALSQGLPDFLAHEPGFYSGLMLSQYTADHLIV
                     EQRMLSAPASIQSIPAAADQEDFVSMGMNTALKTGQILDNAYGVLGIEFMAAAQALDF
                     RTFTIGKGSAAAREVIRRHVRHLDVDRPLFDDHNAMQALVRSGEILEGVERAVGPLA"
     gene            4140..5231
                     /locus_tag="PLT35_03700"
                     /pseudo
     CDS             4140..5231
                     /locus_tag="PLT35_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
     gene            5512..6447
                     /locus_tag="PLT35_03705"
     CDS             5512..6447
                     /locus_tag="PLT35_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28360.1"
                     /translation="MMRVGLLCGREYSFPPAFIAKVTELGAEHGIVGELVRLGGTYMG
                     EAPRYRVIVDRISHEVDYYRGYLKHAVLGGAYVINNPFWWSADDKFFNYAVAARLGVA
                     IPRTILLPQKGYPWDVDIQTESLRNLEYPLDWDAMLEFVGLPAILKPFSGGGWKHVYK
                     VSSKEELWAAYDGTAPYCMTLQEFIDFDQYVRCFTFGKTDIVPVAYDPHERRYLVDPD
                     YLSKEVGERVVRDAQTINAALGYEMNTVEFAIKDGVPYAIDFLNPAPDFERDRITPFY
                     FDMAVERMARLAIDRALRGEASMTSPNWKAMVGLK"
     gene            6444..7802
                     /locus_tag="PLT35_03710"
     CDS             6444..7802
                     /locus_tag="PLT35_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28361.1"
                     /translation="MNPIDAWHGLLRPEELSAGAFEALSARLRAERLTFGDRVHCPFL
                     RPIFVTAEDERRVREFAETLAAVGERVVQAAMADASLVEAVGIREDERRLLAIEPGYA
                     VSSTASRVDAFLLPESLQAAEYNAESPAGFGYAERLAAVFDALPVMARFRERYEARAY
                     GLTAAMLDALLASYRDWGGRASPPTIAIVDWRDVPTWSEFEILQERFTALGAPTVICP
                     PEELEFDGRQLRGAGRRIDLVYRRVLVNDVTARPAACEALVRACEARAVCMANSFRCK
                     IPHKKAFFAVLTDPAFARLFTGAERAIIARHVPWTRAVAEGRTTKGGEEIDLLPWARR
                     HREDLVLKPNDDYGGAGVTLGWELDDTGWDAAIQAALAAPAGSWVVQEKIAVRREVFP
                     WFRDGRDAVLADMLVDMAPYLFRGKLAGFLTRLSATGLANVTSGGGQVPAYFVRPRTP
                     AP"
     gene            7820..8968
                     /locus_tag="PLT35_03715"
     CDS             7820..8968
                     /locus_tag="PLT35_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28362.1"
                     /translation="MPFDCRHRQRFGPGACLSGCPAAPPIFDVVRVGRAGDLPPADPA
                     FIDVAVLDMHCGWPNLGHGAILQAMQNALCDLAGPLAEAGIGFRALSYDVRGGGVVPE
                     PPGDRHQLYIGTGGPGHLDPRMNDGRSDGSQGVVEDPSWEPRVFALFDAIRASRSAAL
                     LAVCHTFGVMCRWLGVADAVLRGPAKGGKSAGIVENVLSGEALAHPWFSAFSKELSDG
                     RRHYVLDSRLYDLVPRAERPAGVAALSHEAADESGSGDQSAAEGGPGGRALTMLEAAR
                     DEGGVMPRILGVNHHPEIVNRPRLLVTLEKEYARGAVSREWYEERRAGLTEPVADERG
                     DGLLHLTSRYTFMGPLRFHLHTLALRRGRDDLRARIGPAHDSVIGRIS"
BASE COUNT         1304 a         3184 c         3284 g         1252 t
ORIGIN      
        1 agtttagcgc gcaaaagcgg ccggccgatc gcggccgccg cggcgatcac cgcgagcagc
       61 gcggcgttgc ccgggttctg cgtggccgcc gctccaatcg ccgccaggct ccaccacggc
      121 gccgattcgg caagcgagaa ggcgacggcg agcagggcga aggtgaaggg ctcggtgtgc
      181 ggcttgtcga tccaccagag gatcggcccg gcgaagacca tggcgcacca ggcccagtgc
      241 aggcgggccg agcacacgcg gaacgccgcg gcgagcagga ggccgttcag gatggtgaag
      301 gcccgcagcg ggcccgaacc gagcgcgccg agcgcggcga ccagcggcgc cgcgagcgcc
      361 gggtaggcga tgaagtgccg cgactcgccg gcgcccggca ggcggcccgc ggccaggcgc
      421 tcggccatgc gcacgtattc ggaggcgtcg ccgacttcgc gcggcgagga cgagaacgcc
      481 agcgccgcca ggagagcggc gaaggcgagg gtggcaacgg cctctcgctt cacggcatgg
      541 ccttcatcga accgcccgcg caccctctgt cccgcaccgc gccttccccg cggaccctcg
      601 aggccgcccg gcgccgcgcg ccggcgcccc gagcttctgc cggagtccgc gagcccgcgt
      661 gcgagcgcgc ccgcatgcac ggcgctgcgc ggcgctgcac gaaacggccc caagctgcag
      721 gccgcccgcc tcggaaccga tacacccacg ggccggcgcg cgggcgccgg cgcgccgggc
      781 gcccgcccgg cttgcaggac ggacgggtac ggccccatag tacggtggaa gccgcgcggc
      841 gacacaaccc gtgtcccggc cgggcccgcg cacccggccc gcaggcggcg cggaagcatc
      901 cggccggcgg agccggcggg tgggaatcgg ctcgaggccg cctccgggcg gctgtctgga
      961 cgacatcatg atcgacacgt cccgcatcct ctgcatcggc gccggctacg tcggcggccc
     1021 gaccatggcc gtcgttgccg ccaagtgccc gcacatccgg gttgacgtgg tggacatcaa
     1081 cgaggcgcgc atcgcggcgt ggaacagcgc cgagctgccc atctacgagc cgggcctgga
     1141 cgaagtcgtg cgcgcggcgc gcggccggaa cctgttcttc tcgaccgaca tcgccggcgg
     1201 catccgcgac gcggacatca tcttcgtctc cgtgaacacg ccgacgaaga ccttcggcca
     1261 gggggcgggc tacgcggcgg acctgcagca ctgggagaag acggcacggc agatcctcgc
     1321 ccacgcgacg cgcgacaaga tcgtcgtcga gaagtccacg ctgcccgtcc ggaccgccca
     1381 ggcgatggag cgcatcctcg acggcaaccc cgccggcgtc cgcttcgacg tcgtgtcgaa
     1441 ccccgagttc ctggccgagg gcagcgccat cgcggacctc gagcggcccg accgcgtctt
     1501 gatcgggggg cacgacaccc ccgccgggcg cgcggcgcgg aggatcatcg cggacatcta
     1561 cgcgacgtgg gtgccgcgcg agcggatcat cgagtcgaac ttgtggagcg cggagctgtc
     1621 gaagctgacc gcgaacgcct tcctcgcgca gcgcatctcg tcgatcaaca gcatctcggc
     1681 gctgtgcgag gtgaccggcg ccgacgtcga cgaggtggcg tacgccatcg ggaccgacag
     1741 ccgggtgggg ccgcgcttcc tgaaggccag cgtcgggttc ggcgggtcgt gcttcaagaa
     1801 ggacatcctg aacctcgtct acatctgccg cagccacggc ctcggggagg tcgccgacta
     1861 ctgggagtcc gtcgtcgcca tcaacgagtg gcagcagtcg cggttcgtgc ggaacatgct
     1921 cgccagcatg ttcaacaccg tcgccggcaa gcgcatcgcg ctgttcggct tcgcgttcaa
     1981 ggccgacacc ggcgacacgc gcgagtcggc cgccatcggc gtggcgcggc agctcctggc
     2041 cgagcgcgcc gttgtcgtca tcaccgaccc gaaggccctc gggagcgtgc gcgaggagct
     2101 gccggaggag ggggaccgga tcgagtgcga gatcgacccc taccgcgcgg cgcggggcgc
     2161 gcacgcgatt gcgatcatga ccgagtggcg gatctaccgg gacctggact acgcgaggat
     2221 cttcgcgtcg atggagaagc ccgccttcgt gttcgacggc cgcaacatcc tggatcacaa
     2281 gcggctgcac gagatcggct tcaacgtcta cgcgctgggc aaggccccgc tgcgccacgt
     2341 gtgacggcgg ggccgggggg ctggaggctg gcgaccgggg actggggcct gggggccggg
     2401 gactgcggtg acagattccg gcttccggct cctggattcc gactcccgga tcctgacatc
     2461 tggctccttt accctctccc cctgtcggac tgtagaatac gtgcgccccc tcgtctcggg
     2521 aaaccgaagg agtcctgctc atggccatcg tgcttgacgg ccgcagtctg acggccgacg
     2581 tgctggttcg cattgcccgg ttcggcgagc ccgtggagct cgcgcccgcc gccctcgagc
     2641 gcatcgcgtc gtgccgcgcg atgctcgagc ggaagctcgc ggcccgggaa gtcatgtacg
     2701 gcacgaacac cggcatcggc gagttctcgg agcgcgtcct gaccgacgag caggtgcagg
     2761 ccttccagcg ctacctcgtg tacaaccacg cggcgggcat cggcgccccg gcgcccgtcg
     2821 agcacgtgcg cggggcgatg gcggggcgca tcaacgtcca tgcccacggc aactcgggct
     2881 gccggcccga gatcacgctg acgctcgtcg agatgctcaa caagggcgtg acgccggtcg
     2941 tctgccagaa gggatcggtc ggcgcgtgcg gcgatctggc gccgatggcg cagcttgcgc
     3001 tcctgctgat gggcgaaggc gaggcgttct accggggcga gcggctgccc ggcgccgagg
     3061 cgatgcgccg cgctggcatc gcggtccccg gcctggaggc gcgcgacggg ctggcgtcga
     3121 tcaacggctc gaacctgctc accgccatgt cggcgctgca cgtctgggac atgcagcgct
     3181 ggctcaggca ggcggagata gccgccgcca tgacgatcga ggcgctcctc ggcaacctca
     3241 agccgtacaa cgcgcgcctg cacgagctgc gcggctttcc gggcgccgtg cagtcggcgg
     3301 cgaacatcat gaaggtgctg gagggctcgg acctcaccac cggcaggatg aagacgaagg
     3361 tgcaggacgc ctactcgatg cgctcgacgc cgcaggtcat cggcgcggcc cgcgacgcga
     3421 ttgcctgggc gcgcgcgcag gtggagacgg agctcaacgg cgtcggcgac aaccccatct
     3481 tcattccgga gcaggacctc acgctcacgg gcgcgaactt ccagggctcg ccggtggcgc
     3541 tgccgatgga catggcgggc gcggccgtca cgatggtgtc ggtcctgtcc gagcgccggc
     3601 tgaaccgcct caccaacccg gcgctctcgc aggggctgcc cgacttcctg gcccacgagc
     3661 cgggcttcta ctccgggctc atgctcagcc agtacaccgc cgaccatctc atcgtcgagc
     3721 agcggatgct gtcggcgccc gcgtcgatcc agtccatccc ggccgcggcc gaccaggagg
     3781 acttcgtgtc gatgggcatg aacaccgcgc tcaagaccgg ccagatcctc gacaacgcgt
     3841 acggcgtgct cggcatcgag ttcatggccg ccgcgcaggc gctcgacttc cggaccttca
     3901 cgatcgggaa gggctcggcg gcggcgcggg aggtcatccg ccggcacgtc aggcacctcg
     3961 acgtggaccg gccgctcttc gacgaccaca acgccatgca ggcgctcgtg cgctcgggcg
     4021 agatcctcga gggcgtcgag cgggcggtgg ggccgctggc ctgagggcgc cggccgcccg
     4081 cgctccggta tgatggagtc tccgccgcgc gccgggcgcg cggcggggag ccaggggcca
     4141 tgccgcaccg attcaccgtt ggcgtcgagg aggagttcca gatcgtcgat cccgcgacgt
     4201 gggagctgcg ctcgcacgtc gcgcagctcc tggagccagg cgcgaccgct ctcggcgagc
     4261 ggatcaggcc cgagctgcac cagtcgatcg tcgaggtcgg cacgaccgtg tgcgccaacg
     4321 ccagcgagct cgaggcgcag gtgcggcaca gccgggagct gacgtccgcc gcctagtccc
     4381 ggggcctcgc catcgcggcc gccggcacgc acgccttctc gcactgggtg gaccaggaca
     4441 tctcctccgg cgaccgctac gcgcacatcg tcgaggagct gcagatgctg gcgcggtcgc
     4501 tcctcatctt cggcctgcac gtccacgtcg gcgtgcccga ccggcagacg gccatcgacg
     4561 tcatgaacat ggcgcggtac ttcctgccgc acctgctggc gctctcgacg agctcgccgt
     4621 tctggatggg gcgcgagacc ggcctcaagt cgtaccgcac gaccgtcttc cgccggttcc
     4681 cgcgcacggg cgtgcccgag cacttcgagt cgtggggcga attcgaggac tacgtgcagg
     4741 cgctcgtcga cctgaagtgc atcgacaacg gcaagaagat ctggtgggac atccgcccgc
     4801 acccgcactt cgggacgctg gagttccgcg tgtgcgacgt gccgacgcgc ccgggcgaga
     4861 cggtggccat cgcgtcgctc atccaggcca tcgtctacaa gctgtacaag ctgcgcgcgc
     4921 agaacatggg cttccgcgag taccgcccgc tcctcatcca ggagaacaag tggcgcgcgg
     4981 cgcggtacgg gatcgacggc aacctgatcg actggggaaa gcgcgccgag gtgccgatgc
     5041 gccggctcgc cctggagctg ctcgatctgg tcgacgacgt ggtcgacgag ctggggagcc
     5101 ggcggcacgt ggcgggcatc ctcgacatcc tgcgcgacgg cacgagcgcg gaccggcagc
     5161 ttcgggtcta ccgcgagacg aaggacctgc gggcggtcgt gcggcacgtg gtggaggaga
     5221 cgaggggcta ggcagccgcc cgcccgcgag cgggtcctgt ccgggacgtt tccatgcgtc
     5281 ctcggcagaa ccgacggcgg ctcgcctgcg ggcacatgga aagcgccccg gccacccgcg
     5341 tggggcgggc gaggcaaggg gctggggacc aggcgctggg cgctagtcag tccagctgtc
     5401 acgcggcggg cctctcacgc ggcgcgagcc gcgtcagact agcccctagc gccgaggccc
     5461 tgttccctgg cggtgacccg ccaccgggcg ggtgtggcaa ggagaagagc catgatgcga
     5521 gtgggattgc tgtgcggccg cgagtattcg ttcccgccgg cgttcatcgc gaaggtcacc
     5581 gagctgggcg ccgagcacgg catcgtcggc gagctggtca ggctcggcgg cacctacatg
     5641 ggcgaggcgc cgcggtaccg cgtgatcgtg gaccgcatct cgcacgaggt ggactactac
     5701 cgcgggtacc tgaagcacgc ggtgctcggc ggggcctacg tcatcaacaa cccgttctgg
     5761 tggtccgccg acgacaagtt cttcaactac gccgtggccg cccggctcgg cgtcgccatc
     5821 cccaggacca tcctgctgcc gcagaagggg tacccctggg acgtggacat ccagaccgag
     5881 tcgctgcgca acctcgagta cccgctcgac tgggacgcca tgctcgagtt cgtggggctg
     5941 ccggccatcc tgaagccctt ctccggcggc ggctggaagc acgtctacaa ggtctccagc
     6001 aaggaggagc tgtgggccgc ctacgacggc acggcgccct attgcatgac gctgcaggag
     6061 ttcatcgact tcgaccagta cgtgcgctgc ttcaccttcg gcaagaccga catcgtgccc
     6121 gtcgcctacg atccgcacga gcggcgctac ctcgtggacc ccgactacct gtcgaaggag
     6181 gtgggcgagc gcgtggtgcg cgacgcgcag acgatcaacg cggcgctcgg ctacgagatg
     6241 aacaccgtgg agttcgccat caaggacggc gtgccgtacg ccatcgactt cctgaacccc
     6301 gcgcccgact tcgagcgcga ccgcatcacc ccgttctact tcgacatggc cgtcgagagg
     6361 atggcgcgcc tggcgatcga ccgcgcgctc cggggcgagg cgtcgatgac gtcgccgaac
     6421 tggaaggcga tggtggggct gaagtgaatc ccatcgacgc gtggcacggg ctgctgaggc
     6481 cggaggagct gtccgccggg gccttcgagg ccctgtcggc gcggctgcgg gcggagcggc
     6541 tgacgttcgg ggaccgcgtc cactgcccgt tcctgcgccc gatcttcgtc accgcggagg
     6601 acgagcggcg cgtgcgcgag ttcgccgaga cgctcgccgc cgtgggcgag cgcgtcgtcc
     6661 aggcggcgat ggccgatgcg tcgctcgtcg aggcggtggg catccgggag gacgagcggc
     6721 ggctgctggc gatcgagccc ggctacgcgg tgtcgagtac cgcgtcgcgc gtggacgcgt
     6781 tcctcctgcc ggagtcgctg caggccgcgg agtacaacgc cgagtcgccc gcggggttcg
     6841 gctacgcgga gcggctggct gccgtgttcg acgcgctgcc ggtcatggcg cgcttccgcg
     6901 agcgctacga ggcgcgcgcg tacgggctga cggccgcgat gctcgacgcg ctgctggcgt
     6961 cctaccgcga ctggggcggc agggccagcc cgccgaccat cgccatcgtc gactggcgcg
     7021 acgtgccgac gtggagcgag tttgagatcc tgcaggagcg cttcaccgcg ctgggcgcgc
     7081 cgaccgtcat ctgcccgccc gaggagctcg agttcgacgg ccgccagctg cgcggggcgg
     7141 ggcggaggat cgacctcgtc taccggcgcg tgctcgtcaa cgacgtcacg gcccggccgg
     7201 ccgcgtgcga ggcgctcgtg cgcgcgtgcg aggcgcgcgc cgtctgcatg gccaactcgt
     7261 tccgctgcaa gatcccccac aagaaggcgt tcttcgccgt cctcaccgac ccggcgttcg
     7321 cgcgactgtt caccggcgcc gagcgggcga tcatcgcgcg gcacgtcccg tggacgcgcg
     7381 ccgtcgccga ggggcggacg acgaagggcg gggaggagat cgacctgctg ccatgggcgc
     7441 gccgccaccg cgaggatctc gtgctgaagc cgaacgacga ctacggcggc gccggcgtca
     7501 cgctcggctg ggagctggac gacacagggt gggacgccgc gatccaggcg gccctcgccg
     7561 cgccggccgg atcgtgggtc gtgcaggaga agatcgccgt gaggcgcgag gtctttccct
     7621 ggttccggga cggccgggac gccgtgctgg cggacatgct cgtggacatg gcgccgtatc
     7681 tgttccgcgg gaagctcgcg gggttcctga cgcggctgag cgccaccggc ctcgcgaacg
     7741 tcacgtcggg cggcggccag gtgcccgcct acttcgtgcg gccccgcacg ccggcgccct
     7801 gagccgcccg cgcccgctca tgcccttcga ttgccgccac cgccagcgct tcggccccgg
     7861 ggcctgcctg tcgggttgcc cggccgcgcc gccgatcttc gatgtcgtgc gcgtcggccg
     7921 ggccggcgac ctgccgccgg ccgatcccgc gttcatcgac gtcgccgtgc tcgacatgca
     7981 ctgcggctgg ccgaacctcg ggcacggcgc catcctccag gccatgcaga acgcgctgtg
     8041 cgacctcgcc gggccgctgg ccgaggccgg catcgggttc cgcgccctgt cctacgacgt
     8101 ccgcggcggc ggcgtggtgc cggagccgcc cggcgaccgc caccagctgt acatcgggac
     8161 gggcggtcca ggccacctcg atccgcgcat gaacgacggc cgctccgacg gatcgcaggg
     8221 cgtcgtcgag gatccgtcct gggagccgcg ggtgttcgcg ctgttcgacg ccatccgcgc
     8281 ctcccgctcg gcggccctgc tggccgtgtg ccacaccttc ggcgtgatgt gccggtggct
     8341 cggcgtcgcc gacgccgtcc tgcgcggccc ggcgaagggc ggcaagagcg ccggcatcgt
     8401 cgagaacgtg ctgagcggcg aggccctggc ccacccgtgg ttctcggcgt tctcgaagga
     8461 gctgtcggac ggccggcggc actacgttct cgacagccgc ctctacgacc tcgtgccgcg
     8521 cgccgagcgg cccgcgggcg tcgccgccct cagccacgag gcggcggacg aaagcgggtc
     8581 gggcgatcaa tccgcggccg agggcgggcc ggggggccgc gcgctgacga tgctggaggc
     8641 ggcgagggac gagggcgggg tgatgccgcg catcctgggc gtcaaccacc atcccgagat
     8701 cgtgaaccgg ccgcggctgc tcgtgacgct ggagaaggag tacgcccgcg gcgccgtgtc
     8761 gcgcgagtgg tacgaggagc gccgcgccgg attgaccgag cccgtggccg acgagcgcgg
     8821 cgatggcctg ctgcacctga cgtcgcgcta caccttcatg ggtccgctgc gcttccactt
     8881 gcacacgctc gccctcagaa ggggtcggga cgatttacgt gcacgaatcg gcccggcgca
     8941 cgattctgtc atcggccgga tttcgtgaca gaggggcccg gcccctccct ggtcctgcgg
     9001 ggaagctggg aggggccggg cccc
//
LOCUS       DAOSIF010000065         8694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7599_length_8694_cov_4.938419, whole genome shotgun sequence.
ACCESSION   DAOSIF010000065 DAOSIF010000000
VERSION     DAOSIF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7599_length_8694_cov_4.938419"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..2494)
                     /locus_tag="PLT35_03720"
     CDS             complement(284..2494)
                     /locus_tag="PLT35_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG28363.1"
                     /translation="MTTRRRLGPLLVCFAAAILACGAPAWAIEGRVIDRRTGEAVRGA
                     EVTILGRTGAVFTDAEGRFTWRPDPRPPFEVLVILPGERFTRPVLIDALPESGPLIVG
                     ISPLVDETVTVTAGAAPDIEATLGSATSFVPARDIVSRQPANLTQALEGVAGVSSVSE
                     GHAAVPAIRGLARGRTLVLIDGARVTSDRRVGPSATFLDPFVLDGVEVARGPGSVAYG
                     SDAFGGVIYARTRRVAPGSPLAVRLVGAVGSGVPQQRAAAEISKGVAKGGFLFQAHAR
                     SFDDYNSPAGEVFNSGAADRGFLARGEHQVGRGILSLSWQSDFGRDIERPRTNSRTVR
                     FVYPVEDSHRIGAAYDLRQAGGFERIVLSAFGGRYRQMTDQDRFATASKGRSLERADY
                     AADDFQIRGTAERLAGNARVEFGVDVNGRVGVRATDTVRQYGLTGAVTSDVVTLSIDN
                     ARRIDAGAYATLEAALVPGAIVGAGIRADRVSTRNIGGYFGDWSTAHGALSGSASLAV
                     GLGKGLSLTGQIGTGFRDPTVSDRYYRGPTGRGFITGNPDLDPERSIQLDAGLRYTSA
                     RVRAGLSAFQYRINDLIERYSTSTDYFFFRNRGRARVRGVEAELHASLPGGLELEATG
                     ALTRGLALDDRTALDDMPPATITAGLRRAVASRGYVRVRGAFFAKDDRPGPTEIVMPG
                     YTLLDALGGVSLSNSLDLNLSIRNLLDRTYPVSPDARAVLAPGLHGVLTIAARF"
     gene            2846..5209
                     /locus_tag="PLT35_03725"
     CDS             2846..5209
                     /locus_tag="PLT35_03725"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOG28364.1"
                     /translation="MPRVFILVRTGLRAAALAALACAWAPPAASAQEPAGAAALAAAP
                     TPEGAGAAPAAPGPGAAPAVVRGADGHVTVRATRISDAIAIDGALDESLYARIPAIDG
                     FLQQEPREGQPATQKTDAWLFYDDRYIYVAARCWSDDPSRIVANELRRDGMALFQNDN
                     FAVLFDTFHDRRNGFMFQTNPLGAVGDTLVTDERDLNRDWNTVWDVRARRFDRGWTLE
                     MAIPFRSLRYPAPGPQEWGVQIRRVARGVNELSYVTPMPAAFAYRAMTRVSQAATLVG
                     LEAPKSGLSLDVKPFALGKVESDLGAAAPFRNDAAAAAGVDVKKTFGNGLVADVTVNT
                     DFAQVEDDEQQVNLTRYSLFFPERRDFFLEGAGIFAFGGASVSPRGGMSQGPPNNTPI
                     LFYSRRIGLYETADGEETGSVPIAGGARLTGRVGAYTIGALGIGQRRAPASGDPATAF
                     SVVRLKRDILKQSSVGVLFTNRSHVPAAGGSNQAAGADAAFTFLKHLTLNAYAARTRT
                     AGVRGNDASYLARAEWMGDRYGAAAEHLVVEKNFRPEAGFLRREDFRRNFGMVRFSPR
                     PANASTIRKFSFEAAADRFANTAGRLETQEIRAEAGVELQNGDEWRLEGRNTYEQLDE
                     AFEITDGIVLPVGGYRYSELEARYMTGGARRLTGSWWVSGGRFYSGTRREAGYRGRLG
                     ITPRIGVEPGVSFNWVKLAEGSFVARLLTARINYAVSARQAFSALLQYNSEGSVVGAN
                     LRFRWEFKPGSDVFIVYNEGRDTTLGIRRSEMSNRSFAIKITRLLRF"
     gene            complement(5261..5896)
                     /gene="msrA"
                     /locus_tag="PLT35_03730"
     CDS             complement(5261..5896)
                     /gene="msrA"
                     /locus_tag="PLT35_03730"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOG28365.1"
                     /translation="MRRKPDAAVMVKPEDALTGRATPMPVPARHAVLGTPMAPPFPAG
                     LETALFALGCFWGAERRFWQVPGVYTTAAGYAGGFTPNPTYQDVCTGLTGHAESVLIV
                     FDPAAVSFEALLRVFWESHDPTQGMRQGADAGTQYRSAIYGATEAQLVAARTSRNAYQ
                     ARLRAAGFGPITTEIAPASGFYYAEEHHQQYLAKNPSGYCGLGGTGVRCPS"
     gene            6054..6362
                     /locus_tag="PLT35_03735"
     CDS             6054..6362
                     /locus_tag="PLT35_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28366.1"
                     /translation="MSAEDDGPKSAYELAMERLRKQDAESGGGLAPLTPRQRAEIAEV
                     RSFYEAKSAECEVLHQSSMRRTLEPEARAELEAQYRRERERLASERDGKIERIRRGSA
                     "
     gene            6466..7839
                     /locus_tag="PLT35_03740"
     CDS             6466..7839
                     /locus_tag="PLT35_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOG28367.1"
                     /translation="MLNRFFAIASRGSSVRREVLGGVTTFVTMAYIIVVNPAILSAAG
                     IPVGPGTVATILSAAFGSILMGLYANRPIAVAPYMGENAFIAFGLMALGATWEQRLGS
                     VFVAGLIFLLLTALQVRSKLAHAISPSLKHSFAAGIGLFLALIGLYETGIVTSGAAGL
                     PASALAVPGQTLLRAPDVPLKIGAFHDPQVLLAVGGFVAIAGLMMRKVNGAILIGMAA
                     AAAAGSAMGFTPAPDSLVALPFTGEYTLAPIALHLDVAGVLRLSFLPVLLTLVLMSFL
                     DTLGTLVGVGAAGGMLDEDGNFPQVEKPMLVDALACVFASLVGSSTSGAYIESAAGIR
                     EGARTGLAAVVTGLLFLASLFLLPLAGPLQQSGYAYGPALVAVGMLMLGSVRKIDFDD
                     LTEALPAFACIALMVFTYNIANGLTAGLVLYPAAKIAAGRARELSRGSVALALLCLTY
                     FVFGLPH"
     gene            complement(8015..>8694)
                     /locus_tag="PLT35_03745"
     CDS             complement(8015..>8694)
                     /locus_tag="PLT35_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28368.1"
                     /translation="RQVAQQLDMTASFLPKPVTGVNGSGMHTNLSLARGQVNLFHDAR
                     GEEGLSAAGWGFVERILASANDICLILNPSVNAYRRLDPRYEAPNQIKASPVNRSAMI
                     RIPLADAKSARLEIRSIAPDANPYLALYSLVRTGLEGPLPEEADTETRRARTRFLPDN
                     IYDAIRHFKASALVTELLGANVQAKYAEVKQLSADRCPKALGSVIKTSEVQFHHEVTN
                     QYLWSRF"
BASE COUNT         1214 a         3154 c         3129 g         1197 t
ORIGIN      
        1 gaaggccagg gacgcgcgcg gcaagatgct gcaggcaggc ccgcgcagag gacccggcgg
       61 ccccggccgc gtcagggcac cgaggccgga cccgaaccag gggcagggcg cgcaggagtc
      121 ccgcgcgctg aagccgcgcc cgggcgcgtc gttcggcctg ccggaaatgg cctgatcccg
      181 acaggcgatc accggcgccg gaggctcccc cctcctcctc cggcgccggt gacgcgccgc
      241 gcgtccgctc tgcttccccc ggctcccgcc ttcgccagcc ccatcagaac cgcgcggcaa
      301 tcgtgagcac gccgtgcagg cccggcgcga ggactgcgcg cgcgtccggg ctcaccggat
      361 acgtccgatc gagcaggttc cggatggaga gattgaggtc aagcgagttc gacagcgaca
      421 cgccgccgag cgcgtcgagc agcgtgtagc cgggcatcac gatctcggta ggccccggcc
      481 tgtcgtcctt cgcgaagaac gcgcctcgca cccggacgta gccccgtgac gcgacagccc
      541 gccgcaggcc cgcggtgatc gtggcgggcg gcatgtcgtc aagcgccgtc cggtcgtcca
      601 gcgccaggcc ccgcgtgagc gcgcccgtcg cctcgagctc gaggccgccc gggagcgacg
      661 cgtgcagctc ggcctcgacg ccgcggaccc tggcccggcc gcggttgcgg aagaagaaat
      721 aatcggtcga ggtcgagtag cgctcgatga ggtcgttgat gcggtactgg aaggccgaca
      781 gccccgcgcg cacgcgcgcc gaggtgtagc gcaggccggc gtcgagctgg atgctgcgct
      841 cggggtcgag atcggggttg cccgtgatga atccccgccc cgtcgggccg cggtagtacc
      901 ggtccgagac cgtcgggtcg cggaacccgg ttccgatctg gcccgtcagc gacaggccct
      961 ttccgaggcc gacggcgagc gacgcgctgc ccgacaaggc gccgtgggcc gtggaccagt
     1021 cgccgaagta cccgccgatg ttcctggtgg acacgcggtc cgcgcggatg ccggcgccga
     1081 cgatcgcccc ggggacgagg gccgcctcga gcgtggcgta cgcgcccgcg tcgatccgcc
     1141 gcgcgttgtc gatcgacagg gtcacgacgt cgctcgtcac cgcgcccgtg aggccgtact
     1201 gccgcaccgt gtcggtggcc cgcacgccga cgcgcccgtt gacatcgacg ccgaactcga
     1261 cgcgcgcgtt gccggccagc cgctcggcgg tgccgcgaat ctggaagtcg tcggcggcgt
     1321 agtcggcgcg ctcgagcgac cggcccttgg acgccgtggc gaagcggtcc tgatcggtca
     1381 tctgccggta ccggccgccg aacgcgctca ggacgatgcg ttcgaagccg ccggcctggc
     1441 ggagatcgta ggcggcgccg atgcggtgcg agtcctcgac gggatagacg aaacgcaccg
     1501 tgcgcgagtt ggtgcgcggg cgctcgatgt cgcggccgaa gtcgctctgc caggacaggc
     1561 tcaggatgcc ccttccgacc tggtgctcgc cgcgcgccag gaagccgcga tcggccgcgc
     1621 cggagttgaa cacctccccg gcggggctgt tgtagtcgtc gaagctgcga gcgtgcgcct
     1681 ggaacaggaa cccgcccttc gcgacgccct tgctgatctc ggccgccgcg cgctgctgcg
     1741 gcacgcccga tccgacggcg ccgaccaggc gcaccgcgag cggcgagccc ggggccaccc
     1801 ggcgcgtgcg ggcgtagatc acgccgccga aggcgtccga tccgtacgcc accgaacccg
     1861 gcccgcgcgc cacttccacg ccgtccagca cgaacggatc gaggaacgtc gcgctcggcc
     1921 ccacgcgccg gtcgctcgtg acccgcgcgc cgtcgatgag gacgagcgtg cggccgcgcg
     1981 ccagaccgcg gatggccggc acggccgcat ggccctcgga gacgctcgac acgccggcca
     2041 cgccctcgag cgcctgcgtc aggttggcgg gctgccggct cacgatgtcc cgcgccggca
     2101 cgaacgacgt cgcgcttccg agcgtggcct cgatgtcagg agccgcgccc gcggtcacgg
     2161 tcaccgtctc gtcgacgagc ggcgagatgc cgacgatgag cgggcccgac tccggaaggg
     2221 cgtcgatgag gaccggccgc gtgaagcgct cccccggaag gatcaccagg acctcgaagg
     2281 gcggcctcgg gtcgggcctc cacgtgaagc ggccctccgc gtccgtgaac acggcgccgg
     2341 tccggccaag aatggtgacc tcggccccgc gcaccgcctc gcccgtccgc ctgtcgatca
     2401 cgcggccttc gatggcccag gccggagcgc cgcaggcgag gatggcggcg gcgaagcaga
     2461 ccagcagcgg acccaggcgg cgtcttgtcg tcatgaagac actccccgcc cgcggcaacg
     2521 ggcgccaggg ggcagatcca atcggcacat cttagccgga atccgcgcgg ccggcgcggc
     2581 ggctgtccgc ggcaggtgtt cggacggatg ccgtgctgag ccgacccaca gcggcaatcc
     2641 gcggcacgac ggcggcagcc ggcccggcgc cgcgcaggcc ggttcgcctc gacggcctcg
     2701 ggagccttga gggccctggc ggcccgcccg gcccggctcg cggacagccg ccaggcgcag
     2761 gcgcgaacgg tcccgtcacg attgcggagc ggcgggccgg tttggcggta ggattagggg
     2821 ctgccgtctc ggcgtggaag atcacgtgcc gcgggttttc atcctcgttc gaaccgggct
     2881 gcgcgcggcg gcgctggccg ccctggcgtg cgcgtgggcg ccgccagcgg cctcggcaca
     2941 ggagccggcc ggcgcggcgg ccttggccgc cgcgccgacg cccgagggcg caggcgccgc
     3001 gccggcggcg ccgggaccag gcgcggcccc ggccgtcgtc cgcggcgccg acggccacgt
     3061 caccgtgcgc gcgacgcgca tctcggacgc gatcgccatt gacggcgccc tcgacgagag
     3121 cctctacgcg cgcattccgg ccatcgacgg cttcctgcag caggagcctc gcgaggggca
     3181 gcccgccacg cagaagaccg acgcgtggct cttctacgac gacaggtaca tctacgtcgc
     3241 cgcgcgctgc tggagcgacg atccgtcgcg catcgtcgcc aacgagctgc gccgggacgg
     3301 catggccctc ttccagaacg acaacttcgc cgtcctgttc gacacgttcc acgaccgccg
     3361 gaacggcttc atgttccaga cgaaccctct cggcgccgtg ggagacacgc tcgtgaccga
     3421 cgagcgcgac ctgaaccgcg actggaacac cgtgtgggac gtgcgcgccc ggcggttcga
     3481 ccggggatgg acgctcgaga tggcaatccc gttccgctcg ctgcggtacc cggcgccggg
     3541 cccgcaggag tggggcgtgc agatccggcg cgtcgcacgg ggcgtcaacg agctctcgta
     3601 cgtgacgccc atgcccgcgg cgttcgccta ccgcgcgatg acgcgcgtgt cgcaggccgc
     3661 cacgctcgtc gggctcgagg cgccgaagag cgggctcagc ctcgacgtga agccgttcgc
     3721 gctcggcaag gtggaatccg atctcggcgc cgcggcgccc ttccgcaacg acgcggccgc
     3781 cgccgccggc gtcgacgtga agaagacctt cggcaacggc ctcgtcgccg acgtgaccgt
     3841 gaacacggac ttcgcccagg ttgaggacga cgagcagcag gtcaacctga cgcgctactc
     3901 gctcttcttc cccgagcggc gcgacttctt cctcgagggc gcgggcatct tcgccttcgg
     3961 cggcgccagc gtcagcccgc gcggcggcat gtcccagggg ccgccgaaca acacgcccat
     4021 tctgttctac agccggagga tcggcctgta cgagaccgcg gacggcgagg agacggggag
     4081 cgtgcccatc gcgggtggcg cgcgcctcac gggccgggtc ggggcgtaca cgatcggcgc
     4141 gctgggcatc gggcagcgcc gggcgccggc gagcggcgat ccggccaccg ccttttccgt
     4201 cgtccggctc aaacgcgaca tcctgaagca gagcagcgtc ggcgttctct tcacgaaccg
     4261 atcgcacgtc cccgccgccg gcggctcgaa ccaggccgcg ggcgccgacg cggcgttcac
     4321 gttcctgaaa cacctgacgc tcaacgccta cgcggcgcgg acgcgcacgg ccggcgtgcg
     4381 cggcaacgac gccagctacc tcgcccgggc cgagtggatg ggcgaccggt acggcgccgc
     4441 ggccgagcac ctcgtggtcg aaaagaactt ccggcccgag gcggggttcc ttcgccggga
     4501 ggacttccgg cggaacttcg gcatggtccg gttcagtccg cgcccggcga acgcgtcgac
     4561 aatccggaag ttcagcttcg aggccgcggc ggaccgattc gccaacacgg ccgggcgcct
     4621 ggagacgcag gagatccgcg ccgaggccgg cgtggagctg cagaacggcg acgagtggcg
     4681 cctcgagggc cggaacacgt acgagcagct cgacgaggcc ttcgagatca cggacggcat
     4741 cgtcctcccg gtcgggggct accgctacag cgagctcgag gcgcgctaca tgacgggcgg
     4801 ggccaggcgc ctgaccggca gctggtgggt gagcggcgga cgcttctaca gcggcacgcg
     4861 cagggaggcc ggataccggg ggcggctggg aatcaccccg cggatcggcg tggaacccgg
     4921 cgtgtcgttc aactgggtga agctggccga aggatccttc gtcgccaggc tgctgacggc
     4981 ccggatcaac tacgccgtgt ccgcccggca ggccttctcg gcgctcctcc agtacaactc
     5041 cgagggcagc gtcgttggcg ccaacctgag gttccgctgg gagttcaagc ctgggtccga
     5101 tgtgttcatc gtctacaacg aggggcgcga cacgacgctc ggcatccggc gatcggagat
     5161 gagcaaccgg tccttcgcga tcaagatcac gcggctgctg aggttctgag atcccgagcg
     5221 ggccgccgcg accgacccgg cgcgcgcgca cgcgttggcg ctaggacggg cagcggacgc
     5281 ccgtgccgcc gaggccacag tagccgctcg ggttcttcgc caggtactgc tggtggtgct
     5341 cctcggcgta gtagaaacca gaggccgggg cgatctccgt cgtaatcggg ccgaacccgg
     5401 ccgcccgaag ccgcgcctgg tacgcgttgc ggctggttcg cgccgcgacg agctgcgcct
     5461 ccgtcgcgcc gtagatcgcc gagcggtact gggtgcccgc atcggcgccc tggcgcatgc
     5521 cctgcgtcgg gtcgtggctc tcccagaaga cccgaaggag cgcctcgaag gacaccgcgg
     5581 ccggatcgaa gacgatcagc acggactcgg cgtggccggt gaggccggtg cacacgtcct
     5641 ggtaggtcgg attcggcgtg aaccccccgg cgtagcccgc ggcggtcgtg tagacgccgg
     5701 gcacctgcca gaaccggcgc tccgcgcccc agaagcagcc cagggcgaag agcgccgtct
     5761 ccaggccggc cgggaacggc ggggccatgg gcgtcccgag caccgcgtgc cgcgcgggca
     5821 ccggcatggg cgtcgcgcgc cccgtcagcg cgtcctcggg cttcaccatc accgccgcat
     5881 ccggcttcct gcgcatgagc ctgctcctgt cttctatgac gctccgcctc cgggattgga
     5941 tctcggccgt ctcgcgtccg ggctcaggcc ccgatggccg cgcggggcac ggccgtcgtg
     6001 ccacgcgcag aaatcggggc ccgaccccgt gtcgccgggg tatcatcccc ctcatgagcg
     6061 cggaagacga cggcccgaag agcgcgtacg agctggcgat ggaacggctg cggaagcagg
     6121 acgccgaatc cgggggcggc ctggcgccgc tgacaccgcg ccagcgcgcc gagattgccg
     6181 aggtgcggag cttctacgag gccaagtccg ccgagtgcga ggtgctccac cagtcgtcca
     6241 tgcggcgcac cctcgaaccc gaggcgcgcg cggagctgga agcgcagtac cggcgcgagc
     6301 gcgaacgcct ggcctcggag cgcgacggaa agatcgaacg catccgccgc ggctccgctt
     6361 gacgccgcgc ggcggcgcgc caggcggcgg cgcgcggcct gtccccggcc cgatcccggc
     6421 tccgactcgg ccgcgccgcc ttcccgggac cggcggagcg cgcccgtgtt gaaccggttc
     6481 ttcgccatcg ccagccgcgg gtcgtccgtg cgccgcgagg tgctcggcgg cgtcacgacc
     6541 ttcgtcacga tggcctacat catcgtggtg aacccggcca tcctcagcgc cgcggggatc
     6601 cccgtcggcc ccggcaccgt ggccacgatc ctgagcgcgg cgttcggctc gatcctgatg
     6661 ggcctgtacg cgaaccggcc gattgccgtc gcgccctaca tgggcgagaa cgccttcatc
     6721 gcgttcggcc tgatggccct cggcgcgacg tgggagcagc gcctcggctc ggtgttcgtg
     6781 gcgggcctca tcttcctcct gctgaccgcg ctccaggtgc gatccaagct ggcccacgcg
     6841 atttcgccga gcctcaagca cagcttcgcg gccggcatcg gcctgttcct cgcgctcatc
     6901 ggcctctacg aaaccggcat cgtcacaagc ggcgcggcag gcctgcccgc gtcggcgctg
     6961 gcggtccccg ggcagacgct gctgagggcc ccggacgtgc cgctcaagat cggcgccttc
     7021 cacgatccgc aggtcctgct ggccgtcgga ggcttcgtcg ccatcgccgg gctgatgatg
     7081 cggaaggtca acggggcgat cctgattggc atggccgctg cggccgcggc cgggtcagcc
     7141 atgggcttca cccccgcgcc ggactcgctc gtggcgctgc cgttcacggg cgagtacacg
     7201 ctcgcgccga tcgcgctcca cctggacgtc gcgggcgtgc tgcggctgag cttcctgccg
     7261 gtcctgctga cgctggtgct catgtcgttt ctcgacacgc tcgggacgct ggtcggcgtg
     7321 ggggccgcgg gcggcatgct cgacgaggac ggcaacttcc cgcaggtgga gaagccgatg
     7381 ctggtcgacg cgctggcgtg cgtgttcgcg tcgctcgtcg gatcgtcgac cagcggcgcg
     7441 tacatcgagt ccgcggccgg catccgcgaa ggcgcgcgca cggggctggc agccgtcgtc
     7501 accgggttgc tgttcctggc gtcgctgttc ctgctgccgc tggccggccc gctccagcaa
     7561 tcgggctacg cgtacggacc ggcgctcgtg gccgttggca tgctgatgct cgggtccgtc
     7621 cggaagatcg acttcgacga cctgaccgag gcgctcccgg cgtttgcgtg catcgcgctc
     7681 atggtgttca cctacaacat cgcgaacggc ctgaccgccg ggctcgtgct ctacccggcg
     7741 gcgaagatcg cggccggccg cgcgcgcgaa ctcagccggg gcagcgtggc gctcgccctc
     7801 ctctgcctga cgtacttcgt cttcgggctg ccgcactgaa cgaaccaggg accagggagc
     7861 gggcgccagg ggctggtcag gtgcgccgcc acgctggcag ggcgctcacg cagccacgca
     7921 gcggggcgag tcacgcgggc ccgttgagcg tccacccacg cggcgacagc cgcgtcgaac
     7981 tggcgcctgg cgccgagccc caggccctag gcgcctagaa cctgctccac aagtactggt
     8041 tcgtcacttc gtggtggaac tggacctcgg aggtcttgat gacggatccg agggccttcg
     8101 ggcaccgatc ggcggacaac tgcttcacct cggcgtactt ggcctgcacg ttcgcgccga
     8161 gcagctcggt cacgagcgcg ctcgccttga aatggcgaat ggcgtcgtag atgttgtcgg
     8221 gcaggaaccg ggtgcgcgcg cgcctcgtct ccgtgtcggc ctcctcgggc agcggcccct
     8281 cgagccccgt cctcaccagg ctgtagaggg caagatacgg gttggcatcc ggggcgatgg
     8341 agcggatctc gaggcgcgcc gacttcgcgt cggccagcgg aatgcggatc atcgcgctcc
     8401 ggttcaccgg cgacgccttg atctggttcg gcgcctcgta gcggggatcg agccgccggt
     8461 acgcgttcac gctcgggttc agaatcaggc agatgtcgtt ggcgctcgcc aggatgcgct
     8521 ccacgaagcc ccagccggcc gccgacagcc cttcctcgcc ccgcgcgtcg tggaacaggt
     8581 tcacctggcc ccgcgccagc gacaggttcg tgtgcatccc gctgccgttg acgcccgtca
     8641 ccggcttggg caggaacgac gcggtcatgt cgagctgctg cgccacctgg cggc
//
LOCUS       DAOSIF010000066         8678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7613_length_8678_cov_4.177316, whole genome shotgun sequence.
ACCESSION   DAOSIF010000066 DAOSIF010000000
VERSION     DAOSIF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7613_length_8678_cov_4.177316"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="PLT35_03750"
     CDS             complement(<1..2223)
                     /locus_tag="PLT35_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28369.1"
                     /translation="MLNRALLHAACALVLLSCGLAARAPQSVTPASAAPPAAGAASAV
                     QPPLAWTDAALQQETYAAPPKELADAVLAPRYRNVALSNASPDKKWFAEEIGDGPVPM
                     AVFAKPFHELGGVFVDFQANRARSLTIRNSAGLQIISAADGARRPVQLPAGVRVSHPT
                     WAPDSSVVGFYVHTPDATHIWVADPVTCAARQATPAPVLATLASGFDFSADGKKLAAI
                     LVPAGRPPMMAPPAAPAGPQVRRAEEKDANRLRTFASLMATPYELALFEWHATGQVAV
                     IDVQPPAAGAARARRPRGAPVPGFVTVGAPAMVRSLDLAPDAKYLRVSRLVKPFSYIV
                     PVANFGSVDEVWDLSGKVLAQLATRPLDEGVQDATSPQPDPGAGPDAADARQQGRREI
                     AWRADGQGLTFLEQDAPAADAAKDGAAAPPPTKGGRGGRAGSESGEGGPGARRPDRLF
                     QWLPPFDQASLKPLFEHSARMSNHRFSPDHQVLFFSERSGRTTVEYAVYLNEPEKRYV
                     LARTRQDDVYSNPGTIVMARGGAGGGRGGAGGPGGRGGAGGPGGGVVQLSADGASVFF
                     QGTLYDKQPEEVGPKTFIDRVAIKTGARTRLYESDNNGVFERVTTPLDIEAGRFIVSR
                     EGPADVAQYYRVDGGTRTRLTANEDVTPDLTRAPRERITVTRADGFSFKARLTLPPGY
                     QAGTRLPAIFWFYPREYASQEEYDRGNQTYNKNEAPVFGTRSMQFFVRLGYAVVEPGA
                     P"
     gene            complement(2353..3783)
                     /locus_tag="PLT35_03755"
     CDS             complement(2353..3783)
                     /locus_tag="PLT35_03755"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOG28370.1"
                     /translation="MRSRQLVVACALALSGCQSSPSTPTPPPCTFTLSATSLSIGASG
                     GAGSVAVSTASSCAWTASSSVSWITATGSTAGTGPGTFSFTVAANTGTSARSGTLTVA
                     GQSVTITQAGLACTYALEPAGRAFGPAGGAGSFEVSTQSGCAWTAAPAAAWLSISAGA
                     SGSGNGTVTYQVAANPDAAPRASSIAVGGASHDVTQSGQAGCAVALDRDRDAFPVSGG
                     SGRVEITASSGCAWAASSDAAWVRITDPAGGSGTGSYRLQYVVDANAGASSRVGTLTI
                     GGKTVTITQAGTASCSYSVAPVDVRACMAEGFSRSIAVTTDDGCPWTASTSAPWIAIS
                     AGSSGTGSGTIVYTLGYNFDAARQAIIEVRWPAPTAGQNVRVAQAGCTYGVSKTSIDV
                     PAAGGDFSFDVISSSTDTSCGGPLQDGCMWSAASSASWVTVTSWMPRWGDDRVSFSVA
                     ANGTGSMRTATITVRDKVVTIRQAGS"
     gene            complement(4048..4491)
                     /gene="dut"
                     /locus_tag="PLT35_03760"
     CDS             complement(4048..4491)
                     /gene="dut"
                     /locus_tag="PLT35_03760"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOG28371.1"
                     /translation="MDAVTVAIVRLRPGVPLPEYQSDGAAGMDLSAALDEPIELRPGE
                     RCLVPCGFAIAVPRGYEAQVRPRSGLAANYGLTLLNSPGTIDSDYRGEVKAVLVNHSN
                     AAFVVTPGMRIAQLVVAPVSRAAWSEAGALPETERGGGGFGHTGH"
     gene            complement(4703..5866)
                     /locus_tag="PLT35_03765"
     CDS             complement(4703..5866)
                     /locus_tag="PLT35_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28372.1"
                     /translation="MSRSGPVKGLLVAVVASVAAVAGAQFHQHRHQRLPIVAGPAVAR
                     VANLSEYGEGLARTMADTKVFFLQGAEPGGSALIMAGTHANEIDALLSALIFVENAVV
                     EKGTLIVIPQLNHSASVYTRPGDGCPLFYGIETPWGTKVFRFGDRGASPLDQWPDPDV
                     YVHYPDAQLLSFLDARNTNRSWPGRPDGLLTERVTAAVMRLLAEERVDIALDLHGAET
                     MFPVTNCIVAPERSMRIATMASMTVTAMEGFENHVEPSPKGFRGLSHREIGDHSDTAP
                     FLLEAPFPFLDQPTGPKTRDLLMTGRDPFLLSLAGQQKLFVPYDESGWPIEKRVGQHC
                     SVALEIINQFSRKRADRAIRISEVPRYADLVRNGVGFYFHDPSKADPARVHLD"
     gene            complement(5945..6100)
                     /locus_tag="PLT35_03770"
     CDS             complement(5945..6100)
                     /locus_tag="PLT35_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28373.1"
                     /translation="MPLVMIVYYVICLFIAGMLVWNFAREKDDRQMMLLYLVVLVPLV
                     LRLLRIN"
     gene            complement(6126..7451)
                     /locus_tag="PLT35_03775"
     CDS             complement(6126..7451)
                     /locus_tag="PLT35_03775"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOG28374.1"
                     /translation="MANPLYAHIAAVLSVMAAAYAAARWRKLGVELCMLAAAVAGGLA
                     GAVVQTPPIGELARHLVEGTFTYLDVMLVFVTATIFITIVTESGGVDFIVRAIVRAFH
                     RQHLVALLLLMILILIPGALTGAGSVSVLTVGAPSALAMGHLGLSKRRTAAILFIVAG
                     LSAAAPPVNIWAMVICAGTAIPYVGFTYALGIPVLLLGTFTILTLGWRRDHRVSRDEA
                     LRAMPQPPPGMNWWRVLLPFGAFFGTVAASRLWPFATPVMGLPLQFTLAAIVALLCSP
                     RRINALALSRDTVRRLVPLLATMAIVGSLQQIMTVTGVRGAISYAVIAMPLALLYVLL
                     VVLLPISEGLLTYGAAAIIGIPLVWYLDSIGLHATIVIAGLSLLWPLGDGLPPTALIG
                     RLTVMVSGYEGSYWSFLKETWLPWLAITAAGIGMIVYSAKLSALVAWGL"
     gene            complement(7562..8227)
                     /locus_tag="PLT35_03780"
     CDS             complement(7562..8227)
                     /locus_tag="PLT35_03780"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOG28375.1"
                     /translation="MRGTWRVAALLPLFLVALATVPFHASGQEAAPKAALPVLTTSAG
                     QSADVNTVNAIMEQAGIKYDYCDVPTAAMLKSGVGLGGAKAKPGFHIESTTDLARFKA
                     GTPYRTVVFALGASLKGMGASGLTVETEVRRVTDLIQYCKQHKIFIVAVHAGGQSKRG
                     APGSDNEKMIDAVAPFADYILVAKDGNKDGRFTKIGQDKGIPVSQVDYALGMVGLITR
                     LFE"
     gene            complement(8274..>8678)
                     /locus_tag="PLT35_03785"
     CDS             complement(8274..>8678)
                     /locus_tag="PLT35_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28376.1"
                     /translation="HWEFDRRSARWAFDYVDFHTQVVYSAAIKDVRKAQETWEKGAMD
                     RTPEIDAQALRLYEKSPEQARRFLTDYCLNNANLVVNAWWNLGDELMVKYMHLWQYDI
                     QRRSRKPLEYPEWWLRELVRYNQLQPQADVKK"
BASE COUNT         1256 a         3088 c         3069 g         1265 t
ORIGIN      
        1 cggggcgccg ggctcgacca cggcgtagcc caggcggacg aagaactgca tcgagcgcgt
       61 gccgaagacc ggagcctcgt tcttgttgta cgtctggttg ccgcggtcgt actcttcctg
      121 gctggcgtac tcccgcgggt agaaccagaa gatggcgggg agccgcgtgc cggcctggta
      181 gcccggcggg agcgtgagcc tggccttgaa cgagaacccg tccgcgcggg tgacggtgat
      241 ccgctccctc ggcgcgcgcg tcaggtcggg cgtgacgtcc tcgttggccg tcaggcgcgt
      301 ccgggttccg ccgtcgacgc ggtagtactg cgctacgtcg gcgggtccct cccgcgagac
      361 gatgaagcgg ccggcctcga tgtcgagcgg ggtggtgacc cgctcgaaga cgccgttgtt
      421 gtcgctctcg tagaggcgcg ttctcgcgcc ggtcttgatg gccacccggt cgatgaacgt
      481 cttcggcccc acctcctcag gctgcttgtc atagagggtg ccctggaaga agacgctggc
      541 tccgtcagcg gacagctgca cgacgccgcc gccgggcccg cccgcgcccc cgcgcccgcc
      601 cggcccgccg gcgccgcccc tgccgccgcc ggcgccgccg cgggccatga cgatggtccc
      661 ggggttcgag tacacatcgt cctgccgcgt ccgcgcgagc acgtagcgct tctcgggctc
      721 gttcaggtag acggcgtact cgacggtggt gcggccggag cgctcgctga agaagagcac
      781 ctggtggtcg ggcgagaagc gatggttcga catccgcgcg ctgtgttcga agagcggctt
      841 caggctcgcc tggtcgaagg gcggaagcca ctggaagagc cgatcgggcc gcctggcgcc
      901 cgggcctccc tcgcccgatt cgcttccggc ccggcctccg cggccgcctt tcgtcggcgg
      961 cggcgccgcc gcgccgtcct tggcggcgtc ggccgcaggc gcgtcctgct cgaggaaggt
     1021 gaggccctgc ccgtcggcgc gccacgcgat ttcccggcgg ccctgctgcc gggcgtcggc
     1081 cgcgtccggc cccgctccgg ggtccggctg gggagaggtg gcgtcctgca cgccttcgtc
     1141 cagcggccgc gtggccagct gggccagcac cttgccgctg aggtcccaga cctcgtccac
     1201 cgagccgaag ttcgcgacgg gcacgatgta ggagaacggt ttgaccaggc ggctcacgcg
     1261 gaggtacttc gcgtcgggcg cgaggtcgag cgagcgcacc atggcgggcg cgccgaccgt
     1321 cacgaaaccc gggaccggcg cgcctctcgg ccgcctggcc ctggcggcgc ccgcggccgg
     1381 cggctgcacg tcgataacgg ccacctgccc cgtggcgtgc cactcgaaca gcgccagctc
     1441 gtagggcgtc gccatgaggc tggcgaaggt ccgcaggcgg ttggcgtcct tttcttccgc
     1501 gcgcctgacc tgggggccgg cgggcgcggc gggcggcgcc atcatcggcg ggcggccggc
     1561 gggcacgagg atcgccgcca gcttcttccc gtcggcgctg aagtcgaagc cggacgcgag
     1621 cgtcgccagc accggcgcgg gcgtggcctg ccgcgccgcg cacgtgaccg ggtccgccac
     1681 ccagatgtgc gtggcgtcgg gcgtgtggac gtagaaaccg accacggagc tgtcgggcgc
     1741 ccaggtcggg tgcgacaccc ggacgccggc cggcagctga accggccgcc tggctccatc
     1801 ggcggccgag atgatctgca ggcccgcgct gttgcggatc gtgagcgacc tggcgcggtt
     1861 cgcctggaag tccacgaaca cgccgccgag ttcgtggaac ggcttcgcga agacggccat
     1921 cggcaccggg ccgtcgccaa tctcctcggc gaaccacttc ttgtccgggc tcgcattcga
     1981 gagagcgacg ttgcggtagc gcggcgcgag caccgcatcg gcgagttcct tcggcggcgc
     2041 cgcgtacgtt tcctgttgca gcgcggcatc cgtccacgcg aggggcggct ggaccgcgct
     2101 cgccgccccg gcggcaggcg gcgcggccga cgccggggtg accgattgcg gggcgcgcgc
     2161 ggcgagcccg caggacagca ggacgagggc gcacgcggcg tgcagcaggg cgcggttcag
     2221 catggcggct cctcgaacac agggaacggc gggaggcggg ggcagcgcca tgctatctgc
     2281 ggcgggagcc gtacacaaga accccgggag ccgcggcccc cggggttcgc gcgggcagga
     2341 gcgggggcgg ccctacgacc cggcctgcct gatggtcaca accttgtcac gcaccgtgat
     2401 cgtcgcagtc cgcatggagc ccgttccgtt ggccgccacg ctgaaggaca cccggtcgtc
     2461 gccccatcgc ggcatccagc tcgtcacggt gacccaggag gccgacgacg ccgcgctcca
     2521 catgcagccg tcctgcagcg gcccgccgca ggaggtgtcc gtcgaactcg agatgacgtc
     2581 gaacgagaag tccccgcccg ccgcgggcac gtcgatgctg gtcttgctca cgccgtaggt
     2641 gcagccggcc tgggcaacgc gcacgttctg tcccgcagtc ggcgcgggcc agcgcacctc
     2701 gatgatggcc tggcgcgcgg catcgaagtt gtagccgagc gtgtagacga tcgttccgga
     2761 gccggtgccg ctcgaaccgg cggagatcgc gatccacggc gcagacgtcg acgccgtcca
     2821 cgggcagccg tcgtcggtgg tgacggcgat cgagcgggag aacccctcgg ccatgcaggc
     2881 gcggacgtcc accggcgcca ccgagtagct gcacgacgcc gttccggcct gcgtgatcgt
     2941 caccgttttg ccgccgatcg tcagcgtgcc gacgcgcgag gaggcgccgg cgttggcgtc
     3001 cacgacgtac tggaggcggt agcttccggt gccgcttccc ccggccggat cggtgatgcg
     3061 cacccacgcg gcgtcgctcg acgccgccca cgcgcagccc gacgaggccg tgatctccac
     3121 cctcccggaa ccgccggaca cggggaacgc gtcgcggtcg cggtcgagcg cgacggcaca
     3181 tcccgcttgt ccggattgcg tgacgtcgtg cgacgcgccg cccacggcga tgctcgacgc
     3241 acgcggggcc gcatcagggt tcgccgcgac ctggtaggtt acggtgccgt tccccgagcc
     3301 cgacgcgccc gccgagatcg acagccaggc cgcggcgggc gcggccgtcc aggcgcagcc
     3361 ggactgggtg ctgacctcga agctgccggc accgccggcc ggcccgaagg ccctgccggc
     3421 cggctcgagc gcataggtgc aggccagccc tgcctgcgtg atcgtgaccg actggccggc
     3481 tacggtgagc gtgccggacc gtgcgctggt gccggtattg gcggccaccg tgaagctgaa
     3541 ggttcccgga cccgtgcccg ccgtgcttcc cgtggccgtg atccagctca cactcgagct
     3601 ggccgtccag gcgcaggagc ttgccgtact gaccgcgacc gaaccggcgc cgcccgacgc
     3661 gccgatcgac agggatgtcg cggaaagcgt gaacgtgcag ggtggcgggg tgggggtgga
     3721 cgggctcgac tggcacccgg acaacgcgag cgcacacgcc acgacgagct ggcgagaacg
     3781 catggctgat cctctctgag ggcccccgcc agtctccctc ccggcgtctg ctctgtcaag
     3841 acgcgaagaa gcttgagaag gccctggaca aaacgcggcc acaacacaaa gagttctgct
     3901 gccgcagacg ttccatcgat cgcggtctgc ccggttactc gccgctggcg cgtgcggccg
     3961 ttctgagaca gccggggctg cggctcggga gccctcgccg ggcgcactcc gggaccggag
     4021 ctccgggacg gtcccgacgc gtcaggctca gtggccggtg tgcccgaatc cgccgccccc
     4081 gcgctccgtt tcgggcagcg cgccggcttc gctccacgcg gcccgcgaca ccggcgccac
     4141 caccagttga gcgattctca tgcccggagt gaccacgaac gcggcattgg agtgattcac
     4201 gaggacggct ttcacctcgc cgcggtagtc gctgtcgatc gtgcccgggc tgttgaggag
     4261 cgtgaggccg tagttcgcgg ccagtcccga gcgcggcctg acctgcgcct cgtacccgcg
     4321 cggcaccgcg atcgcgaacc cgcatggcac gaggcaccgc tcgcccggcc ggagctcgat
     4381 gggctcgtcg agggccgccg agaggtccat gccggcggcc ccgtccgact ggtactccgg
     4441 caggggcacg ccgggcctca acctgacgat tgcgacagtc acggcatcca tgggcgtcac
     4501 cggcattcta ccgcacgcgc gcaggccgcc gggctgctcg ccgacgccgt gcacggcgtc
     4561 cgaacgggcg ccgcgtccgc cgtccgggag ctgtcggccg aggcgccagc cgctgacgcg
     4621 gtgaccgggg gttggccgtc ccgtccgggc tgctcggcag cttgagcctg ccgcccatca
     4681 ggagcctgcc cgtccgcacc cctcaatcga ggtggacgcg cgcgggatcg gccttgctcg
     4741 ggtcgtggaa gtagaacccc acgccgttgc ggacgaggtc cgcgtagcgc gggacctcgc
     4801 tgatccgtat ggcacggtcg gcgcgcttcc tggagaactg gttgatgatc tccagcgcga
     4861 ccgaacagtg ctgtcccacc cgtttctcga tgggccagcc cgactcgtcg tagggcacga
     4921 agagcttctg ctgcccggcc aggctcagca gaaacgggtc cctcccggtc atcagcaggt
     4981 cgcgggtctt cgggccggtg ggctggtcca ggaacggaaa gggcgcttcc aggaggaagg
     5041 gcgccgtatc cgagtggtcc ccgatctccc ggtgcgacag gcctcggaat cccttgggcg
     5101 acggttccac gtggttctcg aagccttcca tggcggtcac ggtcatcgac gccatcgtgg
     5161 cgatcctcat cgaccgctcc ggcgccacga tgcagttggt gaccgggaac atcgtctcgg
     5221 cgccgtgaag gtctagggcg atgtccacgc gctcctcggc gagcagccgc atcaccgccg
     5281 ccgttacccg ctcggtcagc aggccgtccg ggcgcccggg ccacgatcgg ttcgtgttgc
     5341 gcgcgtccag gaacgacagc agctgagcgt ccgggtagtg gacgtacacg tcgggatcgg
     5401 gccactgatc gagcggcgag gcgccgcgat cgccgaaccg gaacaccttc gttccccagg
     5461 gcgtctcgat tccgtagaac aggggacagc cgtcacccgg cctggtgtag acgctcgcgc
     5521 tgtggttgag ctggggaatg acgatcaggg tgcccttctc gacgacggcg ttctccacga
     5581 agatgagggc ggagaggagc gcgtcgattt cgttggcatg cgtgccggcc atgatcagcg
     5641 cgctgccgcc cggctccgcg ccctgcagaa agaacacctt cgtgtcggcc atcgtgcgcg
     5701 ccagcccctc gccgtactcg ctcaggttgg ccacccgggc cacggccggc cccgccacga
     5761 tgggcaggcg ctggtgccga tgctgatgga actgcgcgcc ggccacagca gccacactcg
     5821 caaccaccgc gaccagaagc cccttgaccg gtccactgcg ggacatggcc tcgtctcctc
     5881 cccggcaccg gctctgccgc gcctcgcctc gacgccgcct ggtcctgccc tccgcctgcc
     5941 gccctcagtt gatcctcagg agcctcagca ccagcggcac cagcacgacc aggtacagca
     6001 gcatcatctg ccggtcgtcc ttctctcggg cgaagttcca gacgagcatg cccgcgatga
     6061 acaggcaaat gacgtagtag acgatcatga cgagcggcat gtcccggctc ccgaccgctg
     6121 gcggctcaga gtccccacgc gaccagcgcg ctcagcttcg cgctgtacac gatcatgccg
     6181 attccggcag ccgtgatggc cagccacggc agccaggtct ccttcaggaa cgaccagtag
     6241 gagccctcgt agccggacac catcaccgtc agcctgccga tgagcgccgt cggcggcagg
     6301 ccgtcgccca gcggccacag caggctcaat cccgcgatga cgattgtcgc gtgcaggccg
     6361 atggagtcca ggtaccacac gagcgggatg ccgatgatgg ccgcggcgcc gtacgtcagg
     6421 agcccctcgg agatcggaag gagcacgacg agcagcacgt acaggagcgc cagcggcatg
     6481 gcgatgacgg cgtaggagat cgcgccgcgc acgccggtca cggtcatgat ctgctgcagc
     6541 gacccgacga tggccatcgt ggccaggagc gggacgagcc tgcggacggt gtccctggac
     6601 agcgccaggg cgttgatccg cctggggctg cagaggagcg ccacgatcgc cgcgagggtg
     6661 aactggaggg gcaggcccat gaccggcgtc gcgaacggcc agaggcggga ggccgcaacg
     6721 gtgccgaaga acgccccgaa cggcagcagc acccgccacc agttcatgcc tggcggcggc
     6781 tggggcatcg cccggagcgc ctcgtcccgc gacacccggt gatcgcgtct ccacccgagc
     6841 gtcaggatgg tgaacgtgcc caggagcagc accgggatgc cgagcgcgta ggtgaacccc
     6901 acgtacggaa tcgccgttcc ggcgcagatc accatcgccc agatgttcac ggggggcgcg
     6961 gccgcgctca gcccggccac gatgaagaga atcgccgcgg tccgcctctt cgacaagccg
     7021 aggtgcccca tcgccagggc gctcggcgcc cccaccgtca gcacggagac gctgccggcg
     7081 ccggtcagcg cccccggtat caggatgagg atcatcagca gcaggagggc caccaggtgc
     7141 tggcggtgga acgcgcggac gatggcgcgc acgatgaagt cgaccccgcc cgactcggtg
     7201 acgatggtga tgaagatggt ggccgtgacg aagacgagca tcacgtcgag gtaggtgaag
     7261 gtcccctcga ccaggtgcct cgccagctcg ccgatcggcg gcgtctgtac gacggctccg
     7321 gccagcccgc cggccaccgc ggcggcgagc atgcacagct ccaccccgag cttccgccat
     7381 cgggccgcgg cgtaggccgc cgccatgacg ctcaggacgg cggcgatgtg cgcgtacagt
     7441 ggattcgcca tgtgtgcgac cgcccatcca cgcgatcacg ccgaggccgc ggccgcgcgc
     7501 ccgtcggcgt ccgcggcccg cgggggccgc gccagccgcg cgcgcccgcg acccgggcgg
     7561 gctactcgaa gagcctcgtg atcaggccga ccatccccag ggcatagtcg acctgcgaca
     7621 ccgggatccc cttgtcctgg ccgatcttcg tgaagcggcc gtccttgttg ccgtccttgg
     7681 ccacgaggat gtagtcggcg aagggcgcca ccgcgtcgat catcttctcg ttgtcgctgc
     7741 cgggcgcacc gcgcttggac tgcccgcccg cgtgcacggc cacgatgaag atcttgtgct
     7801 gcttgcagta ctggatgaga tccgtgacgc gcctgacctc ggtctccacc gtgaggccgg
     7861 aggcgcccat ccccttgagg ctcgcgccga gcgcgaagac caccgtgcgg tagggagtcc
     7921 cggccttgaa cctcgcgagg tccgtcgtgc tctcgatgtg gaaccccggc ttcgccttgg
     7981 ccccgccgag gccgacgccg ctcttcagca tcgccgccgt ggggacgtcg cagtagtcgt
     8041 acttgatgcc ggcctgctcc atgatggcgt tgaccgtgtt cacgtccgcg ctctggccgg
     8101 ccgaggtcgt gagcaccgga agggcggcct tgggcgcggc ttcctgcccg gacgcgtgga
     8161 acggaaccgt ggccagcgcc acgaggaaca gcggcagcag agccgcgact cgccaagtcc
     8221 ctctcatacc gggcctcctg tgtgtctgcg cggcagcctc gccggccgcc ctgctacttc
     8281 ttgacgtccg cctggggctg gagctggttg tagcggacca gctcgcgcag ccaccactcg
     8341 gggtactcga gcggcttgcg cgagcgccgc tggatgtcgt actgccagag gtgcatgtac
     8401 ttcaccatca gctcgtcgcc caggttccac caggcgttga cgacgaggtt cgcgttgttc
     8461 aggcagtagt cggtgaggaa ccgcctggcc tgctcgggcg acttctcgta gagccgcagc
     8521 gcctgcgcgt cgatctcggg cgtgcggtcc atggcgccct tttcccatgt ctcctgcgcc
     8581 ttgcgaacgt ccttgatggc ggcggagtag acgacctgcg tgtggaagtc gacgtagtcg
     8641 aacgcccagc gggcggaccg gcggtcgaac tcccagtg
//
LOCUS       DAOSIF010000067         8633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7648_length_8633_cov_3.926906, whole genome shotgun sequence.
ACCESSION   DAOSIF010000067 DAOSIF010000000
VERSION     DAOSIF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7648_length_8633_cov_3.926906"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PLT35_03790"
     CDS             <1..1012
                     /locus_tag="PLT35_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG28377.1"
                     /translation="TTIRSVKYAYWNLKSAAAALDVANQSLDLAREALRNNKSKVEIG
                     TMAPIDVVEAEAEVARRTESVIAAEAGVRSAEDQLRTIILDSKEPDYWTTHFSLTDEP
                     VFAAQTLDVEAVVRTALEKRTDLRQSRRNLEVTRETIKYQRNQTLPDLSAKIGYGLTG
                     QGGTKINFGPGFPPPILDTREEGWDKVISRLVSNDFNNWSVGVSFSYPLGKGAAEAQL
                     ARTRLQLSQAELQLQNLELQVATSVRDVVRNVQTNQLKLASSRATRTLMERRAQAEQK
                     KFAAGLSTNFLVFQAQRDLADAQYAELVALLDYNKSLVDLETVQEAPTAGSAVVQVAA
                     GR"
     gene            1153..2151
                     /locus_tag="PLT35_03795"
     CDS             1153..2151
                     /locus_tag="PLT35_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG28378.1"
                     /translation="MQPQAEVQQGAAVIALDLGGTKLASAMFVGDGRPRLVRVREIGR
                     RQGAGVASLVCGEIRRLQHAAQQRGLQVRAVGVSVPGIAGPRTGRVWAPNIAGWADYP
                     LRDEIAALPETQGAPVVVDSDRAACILGEAWQGAARGCRDAIFLAVGTGIGAGILAGG
                     RVLRGASGSAGSAGWLALGRSFRREFAACGDFEWRASGRGLAASAARTAARTAGYRGP
                     LSKASGLTARDVFAAFDADDPVAAAVIRSAVRSWGVACASLVSLFAPETIVFGGGVFG
                     PAVRWLEAIRAEAARWAQPIAMRRVKFEASRLGPLAALYGAGWLARRAASGRRRPG"
     gene            2163..3344
                     /locus_tag="PLT35_03800"
     CDS             2163..3344
                     /locus_tag="PLT35_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG28379.1"
                     /translation="MYNRNVVFAAACAGMLLFGIVFLSLGSVNNMLAERFLLDMNAIG
                     TLTALLPFGILLGSLVFGPVADRFGYQGLLIACALLVGAGLEGLASADSRRMVQACVF
                     LIGFGGGVLNGATNALASDVSEGDRGARLSLLGVFFGIGALTMPGTLAALSRRYALSS
                     IVSGVGLLALAPAAAFTVIRFPPPKRRGEPMSFTGGFALLKHPVFRLIVLALAVQSGM
                     EGMSNDWMTRYFKLVTLAGRAADEWRAQLGLVAVTAGMVGARLALSGVLARVRSQAVL
                     AVGLALAATGAAVLRQAPCYTASLGAALCLGAGLAPVYPILLSYVGDLFPARSGAAFS
                     TLFTVALVGNMVMNKSFGALAHARGINQYAAVMLGCLALSAGLLILVLKRLRVHRAAE
                     A"
     gene            3350..4117
                     /locus_tag="PLT35_03805"
     CDS             3350..4117
                     /locus_tag="PLT35_03805"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOG28380.1"
                     /translation="MSELALHWLSHARSVMDRIAETQLPSIRKAASVMADSIEAGRWV
                     HLFGCGHATIPVEELYPRIGGFVGFHPMIELPLSFFTRITGEMGVHQFVFLERVEGYG
                     VEIMKGYTFDPRDTMWIFSHSGINSVNIDVALEAKRLGMTLVAYGSAAAAAGRPARHS
                     CGRTIFDVADVVVDTCAPIGDASVPVPGHQDKVGPISTVAFVTSAWMTVCTVAELLAA
                     RGVKLYINPSHNVPGDATARQRLDEALAEYKRRVAGV"
     gene            4198..4971
                     /locus_tag="PLT35_03810"
     CDS             4198..4971
                     /locus_tag="PLT35_03810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG28381.1"
                     /translation="MPGVTATIITLNEAANIAAALESVRWADEIVVVDAESTDDTAAI
                     AKRYADRVIVRAWPGFVDQKNHAASLARHDWVFSLDADERATPELAAEIRGRLASEPA
                     EAGFRVPRVSRYLGRWIRSTDWYPDCQLRLYDRRRARWTGRHVHESVQADGPVGRLCA
                     ELQHHPYRSVSDHLRTIDRYSSLAARQMREDGRSAAAWQIAVHPLAAFVRNYVLRGGF
                     RDGMAGLIVSSLNAHYVRLKFVKLWEAERNPESSSQNPE"
     gene            5055..6185
                     /locus_tag="PLT35_03815"
     CDS             5055..6185
                     /locus_tag="PLT35_03815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28382.1"
                     /translation="MFSLHIDTARTWRGGQNQVLLTVLGLRARGHRAVLVARPGGELA
                     RRASEGHDLVLIAPAHEMDFAAAFRLARVVRDLKPDVVHAHDPHAVSMAAMAISTMAD
                     RRRPPLVIARRVDFHLKRNAFSRWKYQQVDAAICSSNAIRAMLVEDGVAADAAFTVYE
                     GVQVDRIRALPAADLCKELWLPPGSPTIGNVAALVPHKGQRYLIDAAPAVIRAVPDAH
                     VLIFGEGELRPALTRQIRHLGLEHRVQLAGFRPDVLSLLKGLDVFVMCSLTEGLGTSI
                     LDAMAASRPVVGTTAGGIPEAVEDGVTGILVPPHNAAALAAALLRLLGDEALRRRMGE
                     AGLERVREQFSVDRMIDGILAVYEAVAGRRREAGTGSPPPVS"
     gene            complement(6085..7044)
                     /locus_tag="PLT35_03820"
     CDS             complement(6085..7044)
                     /locus_tag="PLT35_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOG28383.1"
                     /translation="MAFQRPARARRRAALTSGPAPGLERLALRSRLEPLYRACNVGGA
                     VADPVEAVRAYTDPADREIAGFIAAGLAFGRVASILVAVTRVLRCMGPSPAAFVRRFD
                     PGRDARRLDGVVHRWIRGRDLSALLLVLRRMLDGAGSIEGFFLSGDDPGAPDIEPGLE
                     SFCARARDIDVRPVYGRRAPRPGVHAFFPLPSAGSACKRLNLYLRWMVRRDAVDLGVW
                     TGVSPARLVIPLDVHVARVGQCLGLTAYRAPGWRMAASITSALRALDPLDPVRYDFAL
                     CRLGMGGLCGFRTPAGDSRCPLRGVCRPRPRKPPGSRRSSGRR"
     gene            complement(7057..7677)
                     /locus_tag="PLT35_03825"
     CDS             complement(7057..7677)
                     /locus_tag="PLT35_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOG28384.1"
                     /translation="MGRREDIRRKAVEDLQRFEVLMLDGHFDYGNGYHGRVYLNPHQL
                     FRYPSTIWRFAQDLIEIMPTGLVEDADVVAGPVTGGALLAHTIAGLLDSRRRLTQPAR
                     LFAPFAPDAQRGHALRSFYRLQIEGRRVLLADDVRNTGQTFERCAALVREAGGTVIGT
                     VEIYDRMEAVVDLGVPNIALAEYRANDNHPAAECPLCREGRPVTAF"
     gene            complement(7679..>8633)
                     /locus_tag="PLT35_03830"
     CDS             complement(7679..>8633)
                     /locus_tag="PLT35_03830"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOG28385.1"
                     /translation="KFESDLEDLDEELAEMADMRLAGCVAFSDDGRPVATALLMRRAL
                     EYAGMLGVPVIDHCEDRTLVGEGVMHEGLHASQLGLRGIPSAAEEVAVHRDIVLAGLT
                     GGHAHIAHLSAAGSLGAVRAAKARGIRVTCEVTPHHFTLTDERLLAPLMYDTNLKMNP
                     PLREASDRDALLEGIRDGTVDAIVTDHAPHHADDKSVEFDRAAFGIVGLETAVSLGLD
                     RLVHSGLIGLGRFVELYSAGPARLLGVAGGRLGVGDPADITILAPDLPVTVSAAAFRS
                     KSRNTPFDGWTLRGGVAATIVGGKPLYVNPDVAQVGGLGRA"
BASE COUNT         1218 a         3121 c         3062 g         1232 t
ORIGIN      
        1 gacgaccatc cggagcgtca agtacgcgta ctggaacctc aagagcgctg cggcggccct
       61 cgacgtcgcg aaccagtcgc tcgacctcgc cagggaagcc ctgcgcaaca acaagtcgaa
      121 ggtcgagatc ggcacgatgg ccccgatcga cgtcgtcgaa gccgaagccg aggtggccag
      181 gcgcaccgag agcgtgatcg ccgccgaagc cggcgtgcgg agcgcggagg accagctgcg
      241 gacgatcatc ctcgactcca aggagccgga ctactggacc acgcacttca gcctgaccga
      301 cgagccggtg ttcgcggcgc agacgctgga tgtcgaggcc gtggtccgga cggccctcga
      361 gaaacggacg gatctccggc agtcgcgccg aaacctcgag gtgacgcgcg aaacgatcaa
      421 gtaccagcga aaccagaccc tgcccgacct gagcgcgaag atcggctacg gcctgacggg
      481 gcagggcggc acgaagatca acttcgggcc cgggttcccg cctccgatcc tcgacacgag
      541 ggaggaaggc tgggacaagg tgatcagccg gctagtcagc aacgacttca acaactggtc
      601 cgtcggggtc tccttctcgt acccgctcgg caagggcgcc gccgaagcgc agctggcgcg
      661 gacgaggctg cagctctcgc aggcggaact ccagctgcag aacctcgaac tgcaggtggc
      721 gacgtcggtg cgcgacgtcg tgcgcaacgt ccagacgaac cagctcaagc tggcgtcgag
      781 ccgcgccacg cgcacgctga tggaacggcg cgcccaggcg gagcagaaga agttcgccgc
      841 cgggctctcc accaacttcc tcgtgttcca ggcgcagcgg gacctggccg acgcacagta
      901 cgcggaactg gtggcgctgc tcgattacaa caagtcgctc gtggacctcg agaccgtcca
      961 ggaggcgccg acggccggct ccgctgtcgt gcaggtagcg gcgggaaggt agggactggg
     1021 cgcagggggc tgggcgccag gcgctaggca aacggggaca aggggtaacg gctggactct
     1081 gaatttcgac tcctgtccct cctttccctt ccctcctgta cttcctcctc ccctcctgct
     1141 aagattcacg ccatgcagcc tcaggcggag gttcagcagg gcgccgccgt catcgcactg
     1201 gatctcggcg gaacgaagct cgcctccgcc atgttcgtcg gcgacggccg tccgaggctc
     1261 gtccgcgtgc gcgagatcgg ccgccgccag ggagcgggcg tcgcctcgct cgtctgcggc
     1321 gagatccgcc gcctccagca cgcggctcag cagcgcggcc tgcaggtccg agccgtcggc
     1381 gtgtcggttc cgggcatcgc cggcccccga acgggccgcg tgtgggcccc gaacatcgcc
     1441 ggctgggctg actacccgct gcgcgacgag atcgcggcgc tgccggaaac gcagggcgcg
     1501 cccgtcgtcg tggacagcga ccgggcggcc tgcatcctcg gcgaggcctg gcagggagcg
     1561 gcccgaggct gccgggatgc gatcttcctg gccgtcggca ccggaatcgg cgccggcatc
     1621 ctcgccggtg ggcgcgtgct gcgcggagcg tccggcagcg ccggctccgc cggatggctc
     1681 gcgctcggcc ggtcgttccg ccgcgagttc gcggcgtgcg gggacttcga gtggcgcgcg
     1741 tcaggccgcg gcctcgcagc ctcggccgcg cgcacggccg cgcgcacggc cggctaccgc
     1801 ggtcctctct cgaaggcttc gggcctcacc gcccgggacg tgttcgccgc attcgacgcc
     1861 gacgacccgg tggcggcggc cgtcatccgc tccgccgtca ggtcctgggg agtcgcgtgc
     1921 gccagcctgg tgagcctgtt cgccccggag acgatcgtgt tcgggggagg tgtgttcggg
     1981 ccggcggtgc gctggctcga ggcgatcagg gccgaagccg cccggtgggc gcagccgatc
     2041 gcgatgcggc gcgtgaagtt cgaggcgtca cggctcggcc ccctcgctgc cctctacggg
     2101 gcgggatggc tggcccggcg ggccgcgtcc ggccggcggc ggccgggatg acggcgcggc
     2161 ccatgtacaa ccgcaacgtg gtcttcgccg ccgcgtgcgc cggcatgctg ctcttcggca
     2221 tcgtgttcct gtcgctcggc tcggtgaaca acatgctggc cgagcggttc ctgctggaca
     2281 tgaacgccat cggcacgctg acggccctgc tcccgttcgg gatcctgctg ggatcgctcg
     2341 tgttcgggcc cgtcgccgac cgcttcgggt accaggggct gctgattgcc tgcgcgctgc
     2401 tcgtgggggc tggcctggag ggcctggctt cggccgacag ccggcggatg gtccaggcat
     2461 gcgtcttcct gatcgggttc ggcggcggcg tgctgaacgg cgccaccaac gccctcgcgt
     2521 cggacgtgag cgagggcgac cgcggcgcca ggctcagcct cctcggcgtc ttcttcggca
     2581 tcggcgccct gaccatgccc gggacgctgg cggcgctgtc gcggcgctac gcgctgtctt
     2641 cgatcgtgtc cggcgtgggc ctgctggcgc tcgcgccggc ggcggcgttc acggtgatcc
     2701 gcttcccgcc cccgaagcgg cgcggcgagc cgatgtcgtt cacgggcggc ttcgcgctgc
     2761 tgaagcaccc ggtgttccgg ctgatcgtgc tggcgctcgc ggtccagagc ggcatggaag
     2821 gcatgtcgaa cgactggatg acgcggtact tcaagctcgt gacgctggcc ggccgggccg
     2881 ccgacgagtg gcgggcgcag ctgggcctgg tggcggtcac cgcgggcatg gtgggcgccc
     2941 ggctggcgct cagcggcgtg ctggcgcgcg tgcgatccca ggcggtgctg gccgtcggcc
     3001 tggcgctcgc ggcaacgggc gccgccgtgc tgaggcaggc gccatgctac accgcctcgc
     3061 tcggcgccgc gctgtgcctc ggcgcgggcc tcgcgcccgt ctatcccatc ctgctgagct
     3121 atgtcggcga cctcttcccg gcaaggtccg gtgccgcgtt cagcacgctc ttcaccgtgg
     3181 ccctcgtcgg caacatggtc atgaacaagt ccttcggcgc gctggcccac gcccgcggca
     3241 tcaatcagta cgccgcggtc atgctggggt gcctcgcgct ctcggccggc ctgctgatcc
     3301 tcgtcctgaa gcgcctgcgc gtccaccgcg cagcggaggc gtgactcccg tgagcgaact
     3361 cgccctgcac tggctctcgc acgcgcggtc cgtcatggac cgcatcgccg aaacccagct
     3421 cccgagcatc cgcaaggccg cctccgtcat ggccgactcc atcgaggcgg gccgatgggt
     3481 gcacctgttc ggctgcggac acgccacgat tcccgtcgag gagctctatc cccgcatcgg
     3541 cgggttcgtc ggcttccacc cgatgatcga gctgccgctg agcttcttca cccgcatcac
     3601 cggggagatg ggggtccacc agttcgtctt cctcgagcgc gtcgagggct acggcgtgga
     3661 gatcatgaag ggctacacct tcgacccgcg cgacacgatg tggatcttct cgcactccgg
     3721 catcaacagc gtgaacatcg acgtcgcgct cgaggcgaag cggctcggga tgaccctcgt
     3781 cgcgtacggc tccgcggccg ccgcggccgg gaggcccgcc aggcactcct gcggccgcac
     3841 gatcttcgac gtcgccgacg tggtcgtgga cacctgcgcg ccgatcggcg atgccagcgt
     3901 gcccgtgccc ggccaccagg acaaggtggg cccgatctcg accgtggcct tcgtgacgtc
     3961 cgcgtggatg accgtgtgca cggtcgcgga gctgctggcc gcgcgcggcg tgaagctcta
     4021 catcaacccc tcccacaacg tgccgggcga tgccacggcg cggcagcgcc tcgacgaggc
     4081 gctcgcggag tacaagcggc gggtggcggg cgtgtagggg ttcgcgggcg gcagtcgcct
     4141 cccttctgga tcctgaattc cgcattccgg aatccgtctt ttgatacgat ccccctcgtg
     4201 ccgggcgtca ccgccacgat catcacgctg aacgaggcgg ccaacatcgc cgccgcgctc
     4261 gagtcggtcc gctgggccga cgagatcgtc gtggtcgacg ccgagagcac ggacgacacc
     4321 gcggccatcg cgaagcgcta cgccgaccgc gtgatcgtcc gggcgtggcc gggcttcgtg
     4381 gaccagaaga accacgcggc gtcgctggcg cgccacgact gggtgttctc cctcgacgcc
     4441 gacgagcgcg cgacgccgga gctcgccgcc gagatccgcg ggcgcctggc gtccgaaccg
     4501 gccgaggccg gcttccgcgt cccgagggtc agccgttacc tcgggcggtg gatccgctcg
     4561 accgactggt atcccgattg ccagctcagg ctctacgacc ggcgaagagc ccggtggacg
     4621 ggccggcacg tccacgagtc ggtgcaggcc gacggccccg tggggcgcct gtgcgcggag
     4681 ctgcagcacc acccctaccg gagcgtgtcc gaccacctcc ggacgatcga ccgctactcg
     4741 tcgctggccg cgcggcagat gcgcgaggac gggcggagcg ccgcggcctg gcagattgcc
     4801 gtccacccgc tggcggcctt cgtgcggaac tacgtcctgc gaggcgggtt ccgcgacggc
     4861 atggcggggc tcatcgtctc gtcgctcaac gcgcactacg tgcggctgaa gttcgtgaag
     4921 ctctgggagg ccgagaggaa tccggaatcc agcagtcaga acccagaata ggagcgcggc
     4981 cagcgtcccg cctgccgaat cctgtattct gaagctgaat tctgaattcc ggattccggc
     5041 tcctggattc cccgatgttc tcccttcaca tcgacacggc ccgcacgtgg cgcgggggcc
     5101 agaaccaggt cctcctgacc gtcctggggc tgcgcgcccg cggccaccgc gccgtgctcg
     5161 tcgcccgccc cggcggggaa ctcgcccgcc gcgcgagcga gggccacgac ctggtgctga
     5221 tcgcgccagc ccacgagatg gacttcgccg ccgcgttccg cctcgcgcgc gtcgtgcgcg
     5281 acctcaagcc ggacgtggtt cacgcccacg atccgcatgc cgtctcgatg gcggccatgg
     5341 cgatctcgac gatggccgat cgccggaggc cgccgctcgt catcgcgcgg cgcgtggact
     5401 tccacctcaa gcggaatgcg ttctccagat ggaagtacca gcaggtggac gcggccatct
     5461 gctcctccaa cgcgatccgg gcgatgctgg tcgaggacgg cgtggccgcc gacgccgctt
     5521 tcaccgtcta cgagggcgtg caggtcgatc gcatccgggc cctgccggcg gccgacctct
     5581 gcaaggagct ctggctcccg ccggggtcgc ccaccatcgg caacgtggcc gcgctcgtgc
     5641 cgcacaaggg ccagcgctac ctcatcgatg ccgccccggc ggtcatccgg gcggtcccgg
     5701 acgcccacgt tctcatcttc ggcgagggcg aactgcggcc ggccctgacg cggcagatca
     5761 ggcacctcgg actcgagcac cgcgtgcagc tggcgggatt caggcccgac gtcctgtccc
     5821 tcctgaaggg gctcgacgtg ttcgtcatgt gctcgctgac ggagggcctc ggcacgtcca
     5881 tcctcgacgc gatggcggcg tccagaccgg ttgtcggcac gacggccggc gggattcccg
     5941 aggccgtcga ggacggcgtc acgggcatcc tggtgccgcc gcacaacgcc gccgcgctgg
     6001 ccgccgcgct gctccggctc ctcggcgacg aggcgctgcg gcggcgcatg ggcgaggccg
     6061 gcctcgagcg ggtgcgcgag cagttcagcg tcgaccggat gatcgacggg atcctggcgg
     6121 tttacgaggc cgtggccggc agacgccgcg aagcgggcac cgggagtccc ccgccggtgt
     6181 cctgaacccg cagagcccgc ccatgccaag gcggcacagc gcgaagtcgt agcgcaccgg
     6241 atcgagcggg tcgagcgctc gcagcgccga cgtgatcgac gccgccatcc gccagccggg
     6301 cgcgcggtac gccgtcaggc ccaggcactg ccccacccgg gcgacgtgga cgtcgagggg
     6361 gatcaccagg cgggcgggcg acacgccggt ccagacgccc aggtcgacgg cgtcgcgccg
     6421 gaccatccag cgcaggtaga ggttgagccg cttgcacgcg ctgccggcgg acggcagcgg
     6481 gaagaacgcg tgcacgcccg gacgcggcgc gcgacgcccg tagaccggcc gcacgtcgat
     6541 gtcgcgcgcg cgagcgcaga acgactcgag gccgggctcg atgtccggcg cgcccggatc
     6601 gtcgccggag aggaagaacc cctcgatcga accggcgccg tccagcatgc ggcgcaggac
     6661 gagcagcagc gccgacagat cccggccgcg gatccagcgg tggacgactc cgtcgaggcg
     6721 ccgcgcatcc cgccccgggt cgaagcgccg gacgaacgcg gctggcgacg ggcccatgca
     6781 gcggagcacc cgggtgacgg ctacgaggat gctcgcgacg cggccgaatg cgaggccggc
     6841 cgcgatgaac ccggcgatct cccgatcagc agggtccgtg taggcccgga ccgcctcgac
     6901 cgggtcggcg accgcgccgc cgacgttgca cgcgcggtag agcggctcca gccggctcct
     6961 gagcgcgagc cgctcaaggc ccggggccgg cccgctcgtg agggccgcac gccggcgcgc
     7021 tcgggccggc cgctggaacg ccatcgtcac ccgcgatcag aacgccgtca ccggacggcc
     7081 ctcgcggcac agcgggcact cggcggccgg gtggttgtcg ttcgcccggt actccgccag
     7141 ggcgatgttc ggcacgccaa ggtccacgac cgcttccatg cggtcgtaga tctcgacggt
     7201 gccgatgacg gttcccccgg cctcgcgcac cagcgccgcg caccgctcga aggtctggcc
     7261 cgtgttgcgc acgtcatcgg ccagcagcac gcgcctgccc tcgatctgca gccggtaaaa
     7321 cgagcggagc gcgtgaccgc gctgcgcgtc tggcgcgaag ggcgcgaaga gccgcgccgg
     7381 ctgcgtcagc cgccggcggc tgtcgagcag gcccgcgatg gtgtgcgcga ggagcgcgcc
     7441 cccggtcacg ggcccggcaa cgacgtcggc gtcctccacg aggcccgtcg gcatgatttc
     7501 gatgagatcc tgcgcgaagc gccagatcgt ggacggatac cggaacagct ggtgcgggtt
     7561 caggtagacc cggccgtggt acccgttgcc gtagtcgaag tgcccgtcga gcatgagcac
     7621 ctcaaaccgc tgaaggtcct ccaccgcctt gcggcggatg tcctctcgcc gtcccacgct
     7681 acgccctccc cagcccgccc acctgcgcca cgtccgggtt cacgtagagc ggcttcccgc
     7741 cgacgatggt ggccgccacg cctccgcgca gcgtccaccc gtcgaagggc gtgttccggg
     7801 acttcgatcg aaacgccgcc gccgacacgg tcaccggcag gtccggcgcg aggatcgtga
     7861 tgtccgccgg atcgcccacg ccgaggcggc cccctgcgac gcccaggagg cgcgcggggc
     7921 cggccgagta gagttcgacg aagcgcccga gcccgatcag cccggagtgc accagccgat
     7981 ccaggcccag cgacacggcc gtctcgaggc cgacaatgcc gaaggccgcc cggtcgaatt
     8041 ccaccgactt gtcgtcggcg tggtgcggcg cgtgatcggt cacgatcgcg tcgaccgtcc
     8101 cgtccctgat gccctcgagg agcgcgtcgc gatcggacgc ctcgcggagc ggcggattca
     8161 tcttgaggtt cgtgtcgtac atcagcggcg cgagcaggcg ctcgtccgtc agggtgaagt
     8221 ggtggggcgt cacctcgcac gtcacgcgga tgccgcgcgc cttggccgcg cggaccgcgc
     8281 cgagcgaccc ggccgcgctg aggtgcgcga tgtgcgcgtg gccgccggtc agcccggcca
     8341 ggacgatgtc gcggtgcacc gccacttcct cggccgcgga cgggatgccc cgcagcccca
     8401 gctgagaggc gtggaggccc tcgtgcatca cgccctcgcc gaccagggtg cggtcctcgc
     8461 agtgatcgat cacgggcacc ccgagcatgc cggcgtattc gagcgcgcgc cgcatcagca
     8521 gcgcggtcgc caccggcctc ccgtcgtccg agaacgcgac gcagccggcg aggcgcatgt
     8581 cggccatctc ggccagctcc tcatccaggt cctcgaggtc ggactcaaac ttc
//
LOCUS       DAOSIF010000068         8597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7685_length_8597_cov_4.160501, whole genome shotgun sequence.
ACCESSION   DAOSIF010000068 DAOSIF010000000
VERSION     DAOSIF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7685_length_8597_cov_4.160501"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLT35_03835"
     CDS             complement(<1..816)
                     /locus_tag="PLT35_03835"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG28386.1"
                     /translation="MIGDVLVAARAITQEQLERAREIQRSTGGPVGDALVAIGAVTEA
                     ALAEALAREARVGFASLDGAEPDADSAARLPERLARGAVLVPIAPRGAARARVAQADP
                     FDVVATDEAQRALGAPVEVVCAPRSAILRLIDAAYGPRAGSRLRALADEAAASLGAAA
                     DAEPLEASPVVRLADALLDDAVASGATDLHLEPDESAVRVRHRVDGVLREADALPRGL
                     QAPVVSRLKVMAGLDIAEQRLPQEGRVTRTVDGRQMDFRVSCFPTVFGEKAAIR"
     gene            complement(826..2241)
                     /locus_tag="PLT35_03840"
     CDS             complement(826..2241)
                     /locus_tag="PLT35_03840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG28387.1"
                     /translation="MFHVLVIDDDEQMQFFLNEALRRQGYAVTLRRTAEEGLQVLKSD
                     AADLVLLDIRLPGMSGLDAVAEILKIDPRAPIIMMTAHGTRDTAIDAIRRGAYDYFTK
                     PFRLEEMEIVIRRALDKRKLYFEIERLREELASAPGHDWARTKSAAMSEVTHLIERAA
                     PTDSTVLIMGESGVGKELVAEWIHERSARKHRPFVRLNCAAIPETLLESELFGYERGA
                     FTGAQQRKPGKFELADGGTLLLDEIGDMTLSTQAKILRVLQSREVERVGGTTTVRVDV
                     RIVASTNKDLLRSVRDGSFRDDLYFRLNVITLHVPSLRERREEIPGLVDHFLAEANKR
                     LGRSIKRLSSDAMAALMAYDWPGNIRELKNAIERAAVVTDGEIVTLSSLPPPIRPAGV
                     GLVAGVASQQWDGPGVLSLDEKMTQLERAFVIEALSRAGGVQAQGARMLGITERSMWH
                     LVKKHRIEVDKIKERAQASGA"
     gene            complement(2294..2815)
                     /locus_tag="PLT35_03845"
     CDS             complement(2294..2815)
                     /locus_tag="PLT35_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28388.1"
                     /translation="MTVRKAHLRLALAALAAAVLYNVWHFALRGRAPSGAAAAREAAE
                     APLIVTPSVAAGPGAAAPPDPLTMPAPPRVDPAARVSFARDPFLFGDESRLVVAAEAP
                     RAPAALPSVRSVLFSAGRRLAMVDGRIVGVGEAAGGYTVADIERDAVVFEAADGSRVR
                     VPLHGPVERAPLR"
     gene            complement(2822..3484)
                     /locus_tag="PLT35_03850"
     CDS             complement(2822..3484)
                     /locus_tag="PLT35_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28389.1"
                     /translation="MTRPPIGRAGEALERLLPFLVIVAVAAAGFMWAVRPRLDAYLAA
                     RADAAALGERVRTLQASIDRSRGLAAADPQAALAEFEARASADDRVADVAALLARAAL
                     DSAPAGQIGGLTIETGDRVPQAAATAAATVRLAGGQARGLPDDGPDARFGLFPYAVSW
                     TPVRVRFDSTFGAIGAFLSTLRDMPTIVEIRSVRLTRGLPLMTAEIVVRVYRRGAPEQ
                     EG"
     gene            complement(3481..5277)
                     /locus_tag="PLT35_03855"
     CDS             complement(3481..5277)
                     /locus_tag="PLT35_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28390.1"
                     /translation="MANRTGIELLPHACRVVTIRTKPKKPPRVVAFHEVMYSPAGAAG
                     LAAELRSAVKGIERKAAVAVWGLNAAHQALRLPPADARDLEVVARREAGAKAPTGFAA
                     PAGPLADAVVIGEIDEQGRREVGYAAAGAEELRSRLQPLVDAGFEIESAVTPALAHAS
                     LVRQRAALPPGAAAAVLSVNATATALSVVRGRVVLLARELPWGGENADADSRGATPAA
                     AALALRLASELRRSFVYVKQHAGIEVSRVFVCGDVTDARALTGPLVQELGIDVETLDV
                     ADQLDDSSLPEPSDRFRSRLAAWQTALALAAEPAPAARLLSSDRRPGFPAARTARRAA
                     GACVAGLLLVAAGWGALSGLTRGVNAEVERLRRAAAALEPEMQRQDEARRQALAASAR
                     SAALEAFASQGPRFAQVLAAFSQAAPAPLALTSLAADPRPGAWEITAEGQVEGPDAAS
                     AQAVFNAFLKALGESPLLGQPAEPPSLRVRTPDAIPEPPPGTAPDPLPGSAEAEAREV
                     EAPRAAPSGPAYIEVARDGRLYRVPLRRQAGSLELERRAEEMRDRQRQAVLARQAPAA
                     AAASRGGGSGGPAPARHPATVIDFTLRYEVAK"
     gene            complement(5457..6104)
                     /locus_tag="PLT35_03860"
     CDS             complement(5457..6104)
                     /locus_tag="PLT35_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28391.1"
                     /translation="MPATARRRGAVGFTLIEVAVVLAVIVTLALVLTPSIANSITDAR
                     MARARNDCHTIANGIYQFFRDTGYFPAWRAAPSAAPGQAPGGANRLDVLVSQGSMPFE
                     DAPSAWTTGRSGSLTDQLVTNAPGYPMRTPMTPFGWNGPYFSSELAADPWGRRYIVNI
                     GAIDLSPGAVTAAGQPKSAVWVLSAGPNGIIETPFAASILASARPGGDDIGTRIQ"
     gene            complement(6110..6865)
                     /locus_tag="PLT35_03865"
     CDS             complement(6110..6865)
                     /locus_tag="PLT35_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28392.1"
                     /translation="MRKLRSRAGLSLVEVTIILLVLMLLTGVLAPSIVDYVRDAQWVK
                     VKEDCEAIGVSVVRMMRDVPPCFRLVGTDGCTVDNRVDLLWSDGATAGLLAPLSPAAT
                     NYTLAGANVNGPLNWDAAITAQDGSMEQHLVRNDLVVPYPAPSVLYPNGIPVGPQHGI
                     GWRGPYLSGPIGPDPWGNAYMVNSVFLTAATDAFAPGEGHAGAAWNRDVFCISSGPNK
                     LFETYFGGCPSTTRFGTCREGDDWIFVIQGGTR"
     gene            complement(7324..8052)
                     /locus_tag="PLT35_03870"
     CDS             complement(7324..8052)
                     /locus_tag="PLT35_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester domain-containing protein"
                     /protein_id="HOG28393.1"
                     /translation="MMSRTIKAAALAGTLSLIVFGAAPAQADPVYLKGYGLGVAGLEV
                     MAPHYFRTFAGQLLLDMGSPDAADDFIAYCIDPTRTRTTIQDMSERALSELPELGNPA
                     AGVQPNAGGRIAWLINTYAGDAWLRANADSADQNARAAALQLAIWEVLFEPFGSYSLT
                     SGSLKLLWAAENHPLILSYSNLYFSSLGANTAEALWYDIRDPQNGYGQDYAGPLPVPE
                     SGSFMLVAAGLLGALRAATRRGRP"
BASE COUNT         1156 a         3121 c         3190 g         1130 t
ORIGIN      
        1 gcggatcgcc gctttctcgc cgaacaccgt cgggaagcac gacacgcgga agtccatctg
       61 gcggccgtcc acggtcctgg taacgcgccc ttcctgcggg agccgctgct cggcgatgtc
      121 gagcccggcc atcaccttca gccgcgacac gacgggcgcc tgcaggccgc gcggcagggc
      181 gtcggcctcg cggaggacgc cgtcgacgcg gtggcggacg cgcacggccg actcgtccgg
      241 ctccaggtgg aggtccgtgg cgcccgaggc gacggcgtcg tccaggagcg cgtcggcgag
      301 gcgcacgacg ggcgacgcct cgagcggctc ggcgtcggcg gcggcgccga gcgacgcggc
      361 cgcctcgtcg gcgagcgcgc gcaggcggga gcccgcgcgg ggcccgtacg cggcgtcgat
      421 gaggcgcagg atcgcggatc ggggggcgca gacgacttcc accggcgcgc cgagggcgcg
      481 ctgggcctcg tcggtcgcga ccacgtcgaa ggggtcggcc tgggccacgc gcgcgcgcgc
      541 cgcgccgcgc ggcgcgatcg gcacgagcac ggcgccccgg gccaggcgtt cggggaggcg
      601 ggcggccgag tcggcgtcgg gctccgcgcc gtcgagggag gcgaagccga cgcgcgcctc
      661 gcgggccagc gcctcggcaa gggccgcctc cgtcacggcg ccaatcgcga cgagcgcgtc
      721 gccgacgggg ccgcccgtgg agcgctggat ctcgcgcgcg cgctccagct gctcctgcgt
      781 gatggcgcgg gcggcgacga gcacgtctcc gatcatcggc gcgcgtcagg ccccggacgc
      841 ctgggcccgc tccttgattt tgtccacctc gatgcggtgc ttcttgacga ggtgccacat
      901 gctgcgctcg gtgatgccca gcatccgcgc gccctgcgcc tgcacgccgc cggcgcgcga
      961 gagcgcctcg atgacgaacg cgcgctcgag ctgcgtcatc ttctcgtcga gcgacagcac
     1021 gcccggcccg tcccactgct ggctggccac gcccgccacc agcccgacgc cggccggccg
     1081 gatgggcggc ggcaggctcg agagcgtcac gatctcgccg tcggtgacga cggccgcgcg
     1141 ctcgatggcg ttcttcagct cgcggatgtt gcccggccag tcgtaggcca tcagggccgc
     1201 catcgcgtcc gacgacaggc gcttgatgga ccggccgagg cgcttgttgg cctcggcgag
     1261 gaagtgatcg accaggcccg ggatctcctc gcgccgctcg cgcagcgacg gcacgtggag
     1321 cgtgatgacg ttgaggcgga agtagaggtc gtcgcggaag ctgccgtcgc gcacgctgcg
     1381 cagcaggtcc ttgttcgtgc tcgcgacgat ccgcacgtcc acccgcaccg tcgtcgtgcc
     1441 gccgacgcgc tcgacctcgc ggctctgcag cacgcgcagg atcttggcct gcgtcgagag
     1501 cgtcatgtcg ccgatctcgt cgagcaggag cgtgcccccg tcggccagct cgaacttgcc
     1561 gggcttgcgc tgctgggccc cggtgaaggc gccgcgctcg tagccgaaca gctccgactc
     1621 gagcagcgtc tcggggatgg ccgcacagtt caggcgcacg aacggccggt gcttccgcgc
     1681 ggagcgctcg tggatccact cggccaccag ctccttgccc acgccgctct cgcccatgat
     1741 gagcaccgtc gaatcggtgg gcgcggcgcg ctcgatgagg tgcgtcacct cgctcatcgc
     1801 cgcgctcttc gtgcgggccc agtcgtggcc cggcgcgctg gccagctcct cgcgcagccg
     1861 ctcgatctcg aagtagagct tccgcttgtc gagggcgcgg cggatgacga tctccatctc
     1921 ctcgaggcgg aagggcttgg tgaagtagtc gtaggcgccg cgccggatgg cgtcgatggc
     1981 ggtgtcgcgc gtgccgtggg cggtcatcat gatgatgggc gcccgcggat cgatcttcag
     2041 gatctcggcg acggcgtcga gcccgctcat gcccggcagc cggatgtcga gcagcaccag
     2101 gtccgccgcg tccgacttga gcacctgcag gccttcctcg gccgtccggc gcagcgtgac
     2161 ggcgtagccc tgccgccgca gcgcctcgtt caggaagaac tgcatctgtt cgtcgtcgtc
     2221 gatcacgagc acgtggaaca taggcgtcca gccacccgca atgggccgcc tcccgggcgc
     2281 ggcccggcct gcatcaccgc agcggtgccc gctccaccgg gccgtgcagc ggaacccgga
     2341 cgcgggaccc gtccgccgcc tcgaagacca cggcgtcccg ctcgatgtcg gcgacggtgt
     2401 agccgccggc cgcctcgccg acgccgacga tcctgccgtc gaccatcgcg aggcgccgac
     2461 cggcagagaa gagcaccgag cgcaccgacg gcagcgccgc cggcgcccgc ggggcctccg
     2521 cggcaacgac gaggcgcgac tcgtcgccga agaggaacgg gtcgcgcgcg aacgagacgc
     2581 gcgccgcggg gtcgacgcgc ggcggcgcgg gcatcgtcag cgggtcgggc ggggcggcag
     2641 cgcccgggcc ggcggccacc gacggcgtca cgatgagcgg cgcctccgcg gcttcgcggg
     2701 cagcggcggc cccggacggc gcgcggccgc gaagcgcgaa gtgccacaca ttgtacagga
     2761 ccgccgcggc cagggcggca agcgcgaggc ggaggtgagc cttgcggacc gtcatggcgg
     2821 cctacccctc ctgctccggc gcgccgcgcc ggtagacgcg gacgacgatt tcggcggtca
     2881 tgagcggcag gccccgcgtc agccggacgg acctgatctc gacaatggtc ggcatgtcgc
     2941 ggagcgtcga caggaacgcg ccgatggcgc cgaaggtcga gtcgaacctc acccgcacgg
     3001 gcgtccagct gacggcgtac ggaaacaggc cgaagcgcgc gtcagggccg tcgtcgggca
     3061 gtccccgggc ctggccgccc gcaagccgca cggtggcggc cgccgtggcg gccgcctggg
     3121 gcacgcggtc gccggtctcg atggtgagcc cgccgatctg gccggccggc gcgctgtcga
     3181 gcgcggctcg cgcgaggagc gcggcgacgt cggcgaccct gtcgtcggcg gacgcgcgcg
     3241 cctcgaattc ggcgagcgcc gcctgcgggt cggccgcggc caggccgcgc gagcgatcga
     3301 tcgaggcctg cagcgtgcgc acgcgctcgc cgagcgccgc ggcatcggcg cgcgccgcaa
     3361 gataggcgtc gagacggggc cggaccgccc acatgaaccc ggcggccgcc accgcgacga
     3421 tgacgaggaa cggcagcagg cgctcgaggg cctcgccggc gcggccgatg ggcgggcgcg
     3481 tcacttcgcc acctcgtagc gcagcgtgaa gtcgatcacg gtggccggat ggcgcgcggg
     3541 cgccgggccg cctgagccgc cgccgcgcga agccgccgcg gctgccggcg cctggcgggc
     3601 caggacggcc tggcgctgcc gatcccgcat ctcctcggcg cggcgctcga gctcgaggct
     3661 gccggcctgg cggcgcagcg gaacgcggta gagccggccg tcgcgcgcca cctcaatgta
     3721 ggccggcccg ctcggcgcgg cgcgcggcgc ctcgacttcc cgggcctccg cctcggcgga
     3781 tccgggaagc ggatctggcg ccgtccctgg cggcggttcg gggatcgcgt ccggcgtccg
     3841 gacgcgcagc gacggcggct cggccggctg cccgagcagc ggcgactcgc ccagggcctt
     3901 taggaacgcg ttgaagaccg cctgcgccga ggcggcgtcc gggccttcga cttgcccctc
     3961 cgccgtgatc tcccacgcgc cgggacgcgg gtcggccgcg agcgaggtga gcgccagcgg
     4021 cgcgggagcg gcctggctga aggcggccag cacctgcgcg aagcgcgggc cctgcgacgc
     4081 aaaggcctcg agcgccgccg agcgggccga ggccgcaagg gcctggcgcc gcgcctcgtc
     4141 ctgccgctgc atctccggtt cgagcgcggc cgcggcgcgc cggaggcgct cgacttcagc
     4201 gttcacgccg cgggtgaggc cgctcaacgc tccccatccc gccgctacga ggagcaggcc
     4261 ggccacacac gcgccggcgg cacggcgggc ggtcctggcc gccggaaacc cgggcctccg
     4321 gtccgacgac agcaggcgcg cggcgggcgc gggctcagcg gcgagcgcca gggcggtttg
     4381 ccacgcggcc aggcgcgagc ggaagcgatc cgacggctcg ggcagcgagg aatcgtcgag
     4441 ctggtccgcc acgtcgagcg tctcgacgtc gatcccgagc tcctgcacga gcggcccggt
     4501 gagcgcgcgc gcgtcggtca cgtcgccgca cacgaagacg cggctgacct cgattccggc
     4561 gtgctgcttc acgtagacga acgatcggcg gagctccgac gcgaggcgga gggcgagagc
     4621 ggccgccgcg ggcgtagcgc cccgggaatc ggcgtcggca ttctcgccgc cccagggcag
     4681 ctcccgcgca agcagcacga cgcggccgcg gaccacgctg agggccgtcg ctgtcgcgtt
     4741 caccgagagc acggccgccg ccgcccccgg aggcagcgcg gcgcgctggc ggaccaggga
     4801 ggcgtgggca agcgccggcg tcacggcgga ctcgatctcg aagccggcgt cgacaagcgg
     4861 ctgcaggcgc gagcgaagct cctcggctcc ggccgccgcg tagccgacct cgcgccggcc
     4921 ctgctcgtcg atctcgccga tgacgaccgc gtcggcgagc ggcccggctg gcgcggcgaa
     4981 ccccgtcggc gccttcgcgc ccgcctcgcg ccgggcgacg acctcaagat cgcgagcgtc
     5041 cgcgggcggc aggcgcagcg cctggtgggc ggcattgagg ccccagacgg cgacggcagc
     5101 cttccgctcg atgcccttca cggccgagcg cagctccgcc gccaggccgg ccgcgccggc
     5161 aggcgagtac atcacctcgt gaaacgccac gacccgcggc ggcttcttcg gcttcgtcct
     5221 gatcgtgacg acccggcagg cgtgcggaag cagttctatc cctgtacggt tcgccatacg
     5281 tcaattggtt tttcggccga aacggggccg ccctctatgg ggtcaggccc gggaaacgca
     5341 cgtaccgccc ggaattcctg ccgctctggg gccgattgcc tgcggatgtc cgcccgctgt
     5401 ggcgtgggat gtcacccctg ccgcttgggc accactttcc tggatctgac cccggactac
     5461 tggatgcggg tgccgatgtc gtcgccgccc ggacgggcgg aggccaggat ggaggcggcg
     5521 aacggcgttt cgatgatgcc gttcggcccg gccgacagga cccagacggc cgacttgggc
     5581 tgcccggcgg ccgtcacggc gccaggcgag aggtcaatcg ccccgatatt gacgatgtag
     5641 cggcggcccc agggatcggc cgcgagctcg gaggagaagt acgggccgtt ccagccaaac
     5701 ggcgtcatcg gcgtccgcat ggggtagccg ggcgcgttcg tgaccagctg gtccgtcagg
     5761 ctgccgcttc ggccggtggt ccaggcgctc ggcgcgtcct cgaacggcat gctgccctgg
     5821 ctgacgagga cgtccagccg gttcgctccg ccgggagcct ggccgggcgc ggccgacggc
     5881 gcggccctcc acgccgggaa gtagcccgtg tcgcggaaga actggtagat gccgtttgcg
     5941 atggtgtggc agtcgttgcg cgcgcgggcc atgcgcgcgt cggtgatgga gttcgcgatg
     6001 gacggcgtca gcacgagcgc gagcgtcacg atcacggcaa gcaccaccgc gacctcgatg
     6061 agcgtgaagc ccactgcgcc tcgccgccgt gccgtcgcgg gcatgactcc tacctggtgc
     6121 ctccctggat gacgaaaatc cagtcgtcgc cctcgcggca ggtgccgaac cgcgtcgtgg
     6181 acgggcaccc gccgaagtac gtctcgaaca gcttgttcgg gcccgaggag atgcagaaca
     6241 cgtcgcggtt ccacgccgcg cccgcgtggc cctcgcccgg cgcgaacgca tcggtggccg
     6301 ccgtcaggaa gacggagttg accatgtagg cgttgcccca ggggtcgggg ccgatcggcc
     6361 ccgacaagta cgggccgcgc cagccgatgc cgtgctgcgg gccgacgggg atgccgttcg
     6421 ggtacagcac gctgggagcc gggtacggca cgacgaggtc gttgcgcacc aggtgctgct
     6481 ccatgctgcc gtcctgggcc gtgatggccg cgtcccagtt cagcgggccg ttcacgttcg
     6541 ccccggccag cgtgtagttc gtggcggcgg gagagagcgg cgccagcagg cccgccgtcg
     6601 ccccgtcgct ccacagcaga tccacccggt tgtccaccgt gcagccgtcg gtgcccacca
     6661 gccggaagca gggcggcacg tcgcgcatca tgcggacgac cgacacgccg atcgcctcgc
     6721 agtcctcctt gaccttcacc cactgcgcgt cccggacgta gtccacgatg gacggggcca
     6781 ggacgccggt gagcagcatc agcacgagga ggatgatcgt gacctcgacg agcgagagcc
     6841 ccgcacgcga cctcagtttt cgcacgcccg tggcctccgg aaaaccctcc atgcggaggg
     6901 gacgccgcct cccgagcgct tccgcagcaa ccgccgtgcc ttcggccgcc accggggctg
     6961 tgcggccctg gacccgggaa ttgcgcttat ttatgacact cgccggctgg gaactccaca
     7021 ggaaagcgcg cctcccgcac gcaattccta ccattcgcgt agctcgaacc aggcgcgccc
     7081 ggggcggccc atcgggccgg accgctctcg cctccgtgcg cgtgcttcat tcaactgctt
     7141 gtcgtgacgc gagttgcggc cgtgaagccc ggcacggccg cacggggcca tcacttcgtg
     7201 taggggcctg accgcccttc ctacaaataa cgtcgcctcg ctcccgtccg tccgggcttc
     7261 ggggagcgtc cccggggccc gctcgggcgg cagcgcggct gcctcggcgg cgccgcgtcg
     7321 ccgctacggc cgcccccgcc gcgtggcggc gcgcagggcg cccagcaggc cggccgcgac
     7381 gagcatgaag ctgccggact caggaacggg cagcggcccg gcgtagtcct ggccgtatcc
     7441 gttctgcgga tccctgatgt cgtaccagag ggcctcggcg gtgttcgcgc cgaggctcga
     7501 gaagtacagg ttcgagtagc tcagaatcag cgggtggttc tccgcggccc acagcagttt
     7561 caggcttccg ctggtgaggc tgtagctgcc gaacggctcg aagagcacct cccagatggc
     7621 gagctgcagg gcggcggcgc gggcgttctg gtcggcgctg tcggcgtttg cgcgcagcca
     7681 cgcgtcgccg gcgtaggtgt tgatcagcca cgcgatgcgg ccgcccgcgt tgggctggac
     7741 gccggcggcg ggattgccca gctccggcaa ctcgctgaga gcgcgttcgc tcatgtcctg
     7801 gatcgtcgtc cgcgtccgcg tcgggtcgat gcagtacgcg atgaagtcgt cggccgcgtc
     7861 gggtgagccc atgtcgagca ggagctgccc ggcgaacgtt ctgaagtagt gaggggccat
     7921 gacttccagg ccggccacgc cgagcccgta gcccttcagg tagaccgggt cagcctgcgc
     7981 gggcgccgcg ccgaaaacaa tcagcgacaa cgtgccggcc aacgccgccg ccttgatggt
     8041 cctgctcatc atccgcgctc cttccgacac ctgcagcgtc ggctgccccc ctggtgagca
     8101 acgagcgtgc ccactggagg aacgggcaat atcgaaggat ttcttcggaa atccgccgat
     8161 ctctccccgt gtcgctcgcg ccgacgccgt gcgggccacc gggcgtgaag gcagaaggcg
     8221 ggcgcgcgcg ggcccgaacc ggcggcggca ccagggaaac ggcggcgcga gcgcagccca
     8281 aagcattcct ggcattgaac ttacgtccgc cttgccgcgc gccgcgccgc cttcgaccgt
     8341 gccgtgttgt ctcacttcct gacactcgac cttctcggga gggtctgcgc agggccgctg
     8401 gggcgccgga gggttcggcg gcccgtcgcg ccgcacgccg cggggatgca acgcgcggcg
     8461 gcgagggcgg acgatggggc gtcgcggcga gagggcgggc ctcagcgcgt gtcgcctgaa
     8521 atgaggaaga cgatgtcgtc gccgcggcgc agcgtgccgt gcgagacgtt gccgccgaac
     8581 gaggtctcga acaggcc
//
LOCUS       DAOSIF010000069         8588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7695_length_8588_cov_5.268721, whole genome shotgun sequence.
ACCESSION   DAOSIF010000069 DAOSIF010000000
VERSION     DAOSIF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7695_length_8588_cov_5.268721"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1413)
                     /locus_tag="PLT35_03875"
     CDS             complement(61..1413)
                     /locus_tag="PLT35_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOG28394.1"
                     /translation="MIDAIQRANALVNGYVWGWPMILLLMGTGLLLTVLTGVVQVRRL
                     GFALTEVLGKLTRHGGGAGSVRPFQAVATALASTVGVGNIAGVATAISIGGPGSVFWL
                     WLSGLFGMATKYAEIVVALHYREPDDSGTMRGGAMYVLRKGLGLPWLGAVFALLTSVA
                     AFGIGNMVQANSVADALRATFGTPTSTTGIGLVILTAVVILGGIKSIARVTEWLVPFM
                     AILYLGGGMVVLVMYAGELPRALGLIVDGAFTGSAAVGGFAGSTIMMAMRYGIARGLF
                     SNEAGLGSAPMVHAAADTDHPVRQGMYGIFEVFVDTILICSTTALVILVTGVWHGGLT
                     GAALSAKAFETGLPGTFGDIVVTGGLLMFAFSTLIGWSYYGETGIVYLLGTKAALPYR
                     LLWLVFVYLGATGSLHLVWDVADTLNGLMAIPNLIGVLVSIPLLLRLQKEFFSRPPGR
                     "
     gene            1710..2516
                     /locus_tag="PLT35_03880"
     CDS             1710..2516
                     /locus_tag="PLT35_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG28395.1"
                     /translation="MTRIPGALRTCWIVLLAVLASAAASRQAAEPARLRVLTYNIHHG
                     EGTDGRIDLARTAAVIARAAPDVVALQEVDNQTTRAHGVDQAAELGRLTGMHAAFGQA
                     MAYAGGGYGDAILSRFPLSAVRVHDLPTAPGAEPRCAIAARVEAGGEGRAFVVVSTHL
                     EHAQAPIRLLQAQALDRALAAAEAWPVILAGDFNDAPEGPAIAALRPQWADASAGQPD
                     PTWPADRPQVKIDYVFFRPAAAWRVVETHVIDELVASDHRPLLVVFEAMP"
     gene            2953..3543
                     /locus_tag="PLT35_03885"
     CDS             2953..3543
                     /locus_tag="PLT35_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG28396.1"
                     /translation="MARETGTGITALLAGWKAGDPSAFDRLVELAYPELRRIAHQQLG
                     WRRPGESLESAALASEVYLKLLRAGSLDCENRVHFLALCAQVMRRILVDHARQRAAAK
                     RGGEAVRVPLDEAAAAVRGADAVDAIDLDAALESLARLDPRKSRLVELRYFAGLTNEE
                     AARVLEISAETAKRDWRMAKAWLLAQLSQAPGAARL"
     gene            complement(3564..6485)
                     /locus_tag="PLT35_03890"
     CDS             complement(3564..6485)
                     /locus_tag="PLT35_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG28397.1"
                     /translation="MNARRSSRPRPALRYAARSSDANWGATASSRSSAPAGWARCTAP
                     TTTSSGAVAIKLLPPEFARDAGHLARIHREARTLATLNHPGIAAIYGLEESDGLDYLV
                     LELVEGEALRGPLDADTALRYACQVASALEAAHAHGIIHRDLKPANIRVTPDRKVKVL
                     DFGVAKAGASGADSPALLAGAAPETLAGLIVGTPGYMSPEQARGLEVDARADIWAFGC
                     LCWELLSGARAFPGDTDADAMTAVLEREPDWGALPAGTPRAVRTLLHRCLEKDASRRP
                     GRMSDVRAALEDILRPPAWRRAAAWLRQPRHAIPAAVLLTLLALIGARVSRRDDRRRW
                     VTEQAVPAIVKLQDAGDYRAASRLLRRAELVAPDDPALRRASLRTAMPLGVTTTPPGA
                     EVSATGFAPDDEDWVRLGTTPLTVSRLSIGFYRIRVHKPGFEPIEASCEVRGGSVIGF
                     ELDPAGSLPPGMVRVPAGTASGSRLDDAKVGSFLIDRFEVTNRQFKAFVDAGGYRTRR
                     FWKEAFTQDGRVLPWDEATRAFRDSTGQPGPSTWAEGTYASGHDKDPVGGVSWFEASA
                     YAEFAGKRLPSVYHWQRAASPGWFSEILEYSNFNGTGPAPVGSYRGLGAYGTLDMAGN
                     VKEWCANEVSGKRITRGGAWNQAAWLFAELDGRSPWDRSAEYGFRCIRDAAPDPGLNA
                     PVGLEPPGRLDEEPVSEEAFTLYRGVYAYDRAPLDARADGAPTETNDWRREIVSFASP
                     VASERITAYVYAPKHAAPPYQAVIYANSGMALRLPSSEPGEERLFEFIVKSGRAFLHP
                     VLKGYYQRRYAAPAAGPNEERDRLLAESKEFRRCLEYLASRGDVDRERLAVFGVSRGG
                     SLVPILAVGDERLKAAVLFSVGLDARRSRRPEADTLNFLPRFTVPTLMAAGRYDFWFP
                     LEASQRPMLTLLGAGDQDKRMIQWEGGHGDMAPHYPSLTREALAWLDRYLGPPKPQAG
                     G"
     gene            complement(6758..7264)
                     /locus_tag="PLT35_03895"
     CDS             complement(6758..7264)
                     /locus_tag="PLT35_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28398.1"
                     /translation="MKRHSVRIAWAVAIVVGLAGTAAAQSHPACSNKTLRGVWGYTET
                     GWVIAPTPTGGTVTVTAAAVGRYGFDGAGHFDGKQNSSANGAVGADTKEGTYEVYPDC
                     TGTLTLTAYRDGVPQRLSVWTFVLVDSGFELRGIMTSMTLPNGTPLSPIMTMTAQQVR
                     SGRPDDKW"
     gene            7791..>8588
                     /locus_tag="PLT35_03900"
     CDS             7791..>8588
                     /locus_tag="PLT35_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28399.1"
                     /translation="MDQVSEPRRTRKVGPMLIRRALRAALRVLFRPGRLLPVLLAALP
                     LAVVSGGQDLGQLKRRLTGVAENVTLRQLLVGRPLTTTIDDAVYDVPFLDPYDPPDSQ
                     DITVLPQAPDGGRLLRPGSFVYLARSYCVGVGARRPWRGDGYAYAPMKGPQADSIRSI
                     IRNSARFPDIPQEEIQKLLWGIIARSSWRDLSAEQKAIAARLLSSRQIALLNGSALEL
                     LPGSVLDRSLAGASPAVARILGAEARMREIFASGVATYEELEEVAVSA"
BASE COUNT         1256 a         3067 c         3029 g         1236 t
ORIGIN      
        1 cgctggtcga aactggggga gtggctggcg ggaggcccgc gatgagccgc cggcccgacc
       61 ctacctgccg ggcggcctgc tgaagaactc cttctgcagc cgcagcagca gggggatgga
      121 caccagcacc ccgatcaggt tggggatggc catcaacccg ttcagggtgt ctgcgacgtc
      181 ccacacgagg tggagcgacc cggtggctcc caggtagacg aacaccagcc acagcagccg
      241 gtagggcagc gccgccttcg tgccgagcag gtagacgatg cccgtttcgc cgtagtagct
      301 ccagccgatc agcgtcgaga aggcgaacat caacagcccg ccggtgacga cgatgtcgcc
      361 gaaggtgccg gggaggcccg tctcgaaggc cttcgccgag agggccgcgc cggtcaggcc
      421 gccgtgccag acgccggtca ccaggatgac gagggccgtc gtcgagcaga tcaggatcgt
      481 gtccacgaac acctcgaaga tgccgtacat gccctggcgc accgggtgat cggtgtcggc
      541 cgcggcgtgc accatcggcg cgctgcccag cccggcctcg ttcgagaaca ggccgcgcgc
      601 gatgccgtag cgcatcgcca tcatgatcgt gctgccggcg aatccgccca cggccgcgga
      661 cccggtgaac gcgccgtcga cgatcaggcc cagcgcgcgg ggcaactccc cggcgtacat
      721 cacgagcacg accatcccgc cgcccaggta gaggatggcc atgaagggca ccagccactc
      781 ggtgacccgc gcgatcgact tgatcccgcc caggatgacc accgccgtga ggatgacgag
      841 cccgatgccg gtcgtgctcg tcggagtgcc gaaggtggcg cgcagcgcgt cggccaccga
      901 gttggcctgc accatgttgc cgatgccgaa ggcggccacc gacgtcagca gcgcgaagac
      961 ggcgccgagc cacggcaggc cgaggccctt cctcaggacg tacatcgcgc cgccgcgcat
     1021 cgtgcccgag tcgtcgggct cgcggtagtg cagcgccacc acgatctcgg cgtacttcgt
     1081 ggccatgccg aagaggcctg acagccacag ccagaacacc gacccgggcc cgccgatgga
     1141 gatggccgtc gcgacgccgg cgatgttgcc gacgccgacc gtcgacgcga gcgcggtcgc
     1201 caccgcctgg aacggcctga cgctgccggc gccgcccccg tgcctggtga gcttgcccag
     1261 cacttccgtc agggcgaagc cgagccggcg gacctgcacg acgccggtca ggaccgtcag
     1321 cagcagcccg gtgcccatca ggagcaggat catcggccag ccccagacgt acccgttcac
     1381 aagagcgttc gcgcgttgga tggcgtcaat catggcgtgt ccgaaggagt gtatcgcaga
     1441 cgccgccccg aacgaggaag cggcccggat gaaaaccgcc gccgggctcg ccgccgatcc
     1501 tgccactgcg gcgccggccg gccgttacac gagccggaca tcgacgtgca aagacgaggc
     1561 gcgttcgaag cgaacgccct cggtgcgtat ccgcggaccg cagccgcgct gacacgcggc
     1621 ctgacctccg tcgcggtata gtcccggggt caggcccgcg gatttccgga tctgaccccg
     1681 actcaaacgg ctgaagaggg agtgctgcca tgacgaggat tcccggagcg ttgagaacct
     1741 gctggatcgt gctgctcgcc gtcctggcga gcgcggccgc ctcacgccag gcggcggagc
     1801 cggcgcggct gcgggtgctg acctacaaca tccaccacgg cgagggcacg gacggccgga
     1861 tcgatctcgc gcggacggcg gccgtcatcg cgcgcgcggc gccggatgtg gtggcgctcc
     1921 aggaggtcga caaccagacc acgcgcgccc acggcgtcga ccaggcggcc gagctgggga
     1981 ggctgacggg gatgcacgcc gccttcggcc aggcgatggc ctacgcgggc ggcggctatg
     2041 gcgacgcgat cctgtcgcgg ttcccgctgt cggcggttcg cgtccacgac ctgccgacgg
     2101 ccccgggagc cgaaccccgc tgcgcgattg ccgctcgcgt cgaggccggc ggggagggcc
     2161 gggcgttcgt cgtcgtcagc acgcacctcg agcacgccca ggccccgatc cgcctgctcc
     2221 aggcgcaggc gctcgaccgg gcgctggcgg cggcagaggc gtggccggtg atcctggcgg
     2281 gcgacttcaa cgacgcgccc gagggcccgg cgatcgccgc cctgcggccg cagtgggccg
     2341 acgcctccgc cggccagccc gacccgacgt ggcccgccga ccgtccgcag gtgaagatcg
     2401 actacgtttt cttccgcccg gccgccgcct ggcgcgtcgt ggagacgcac gtcatcgacg
     2461 agctcgtggc gtccgaccac cggccgctgc tcgtcgtgtt cgaagccatg ccgtgagcgg
     2521 tcctgcggca gggtctccgc aggaggatcg ccggcctcgc cagagccagt ttccgcgccg
     2581 cggcggcggg gttcgacgcg gccaccggcg tctgcgcgat cgtcctcacg ctggagagcc
     2641 gatgggcgga cggccggttc tcgggcggcg tcgcgcgctc gctcgacggc ggcgagtcgt
     2701 ggcggcggat cctcgtcggc acgcccgaac ggccggactg caacgtcgag cccggctggc
     2761 tgaccgaaga gatgtcctgg tggtgggtcg gcctggcgtg cgggttctgg tgcgatcccc
     2821 gcaatcccgg cgacctcctc ttcaccgact ggggccgcgc cattcgctcc accgacggcg
     2881 gcgcgacgtg gagcaccgcg ttcgcggggc gctaccgctg tgctgagtgt agaatgcctc
     2941 ggcggtgagg gcgtggcgag agaaaccggc accgggatca cggcgctgct cgccggctgg
     3001 aaggccggcg atccgtccgc gttcgaccgc ctcgtggagc tggcctaccc ggaactgcgc
     3061 cgcatcgcgc accagcagct cggctggcgt cgtccgggcg agagcctcga gtccgccgcc
     3121 ctcgccagcg aggtctatct caagttgctg cgcgcgggga gcctcgactg cgagaaccgg
     3181 gtgcatttcc tcgcgctctg tgcgcaggtc atgcgccgca tcctcgtcga ccacgcccgg
     3241 cagcgcgcgg cggccaagcg cggcggcgag gccgtgcggg tgcctctcga cgaggccgcg
     3301 gcagccgtcc gcggcgcgga cgccgtcgac gcgatcgacc tcgacgccgc gctcgaatcc
     3361 ctggcccgcc tcgacccgcg gaagagccgc ctcgtggaac tccgctactt cgccggcttg
     3421 accaacgagg aagcggcgcg agtcctggag atctcggccg aaaccgccaa gcgcgactgg
     3481 cgcatggcga aggcctggct gctcgcgcag ttgtcccagg cgcctggcgc cgcgcgcctc
     3541 tagggccggc cgcccctgcg gcgtcaccct ccagcctgtg gtttcggggg cccgagatag
     3601 cggtccagcc aggcgagggc ctcgcgggtc agcgacgggt agtgcggcgc catgtcgccg
     3661 tggcccccct cccactggat catccgcttg tcctggtcgc ccgccccgag cagggtgagc
     3721 atcgggcgtt gcgacgcctc gagcgggaac cagaagtcgt agcggcccgc tgccatgagc
     3781 gtgggcacag tgaagcgcgg caggaagttg agcgtgtccg cctctgggcg ccgggaacgc
     3841 ctcgcgtcca ggccgacgct aaacaggact gcggctttca gccgctcgtc cccgactgcg
     3901 agaatcggca cgagcgagcc gccgcggctg acgccgaaaa cggccaaccg ttcgcggtcc
     3961 acgtcgccgc ggctcgcaag gtattcaagg caacggcgga actccttcga ctcggccagc
     4021 aggcggtccc gctcttcgtt cgggccggcc gccggggcgg cataacgccg ctggtagtag
     4081 cccttcaaca caggatggag gaacgcccgg ccgctcttga cgatgaactc gaacaggcgc
     4141 tcttcgccgg gctccgacga aggaagcctc agcgccatgc ccgagttcgc gtagatcacc
     4201 gcctggtagg gcggcgccgc atgcttgggc gcgtagacgt acgccgtgat gcgctcgctg
     4261 gccaccggcg aggcgaacga gacgatctcg cgtcgccagt cgttcgtctc ggtcggtgcg
     4321 ccgtcggcgc gcgcatcgag cggggcccgg tcgtaggcgt agacaccgcg gtagagcgtg
     4381 aacgcctcct ccgaaacggg ctcttcgtcg agtcggcccg gcggctccaa cccgaccggc
     4441 gcgttcagac cggggtccgg ggccgcgtcg cggatacagc ggaagccgta ttccgccgac
     4501 cggtcccacg gcgaccgccc gtcgagctcc gcgaacagcc acgcggcctg gttccaggcg
     4561 ccgccgcgcg tgatccgctt ccccgacacc tcgttcgcgc accactcctt cacgttgccc
     4621 gccatgtcga gcgtgccgta tgcgccgagg ccacggtagg aaccgaccgg cgccgggccc
     4681 gtgccgttga aattgctgta ctccaggatc tcggagaacc agcccgggct ggcggcgcgc
     4741 tgccagtggt aaaccgaggg caggcgcttg ccggcgaact cggcgtacgc cgaggcttcg
     4801 aaccagctca cgccgccgac cgggtccttg tcgtggccgc tcgcgtaggt gccctcggcc
     4861 caggtcgatg ggccaggctg cccggtcgaa tcccggaacg cgcgggtcgc ctcgtcccac
     4921 gggagcactc ggccgtcctg cgtgaaggcc tccttccaga accggcgcgt cctgtagccg
     4981 ccggcgtcca cgaatgcctt gaattgcctg ttcgtgactt cgaaccggtc gatcaggaag
     5041 ctgccgacct tcgcgtcatc gagcctggat ccgctcgccg ttccggccgg gacgcgcacc
     5101 atgcctggcg gcaggctgcc agccgggtcg agctcgaacc cgatcacgct gccgccacgc
     5161 acttcgcagc tcgcctcgat gggctcgaat cccggcttgt gaacgcggat gcggtagaac
     5221 ccgattgaga gccggctgac ggtgagcggt gtcgtaccga gtcgcaccca gtcctcatcg
     5281 tcgggcgcga agccggtggc cgagacctcg gcgccgggcg gcgtcgtagt gacgccgagc
     5341 ggcatcgccg tcctgagcga agcccgccgg agggcggggt cgtccggcgc gacgagctct
     5401 gcgcgacgca gcaggcgcga cgccgccctg tagtctccgg cgtcctggag cttgacgatc
     5461 gccggcaccg cttgttccgt gacccaccgg cggcgatcgt cgcggcgcga cacgcgtgcg
     5521 ccgatgagtg ccagcagcgt cagcagcacc gccgcgggga tcgcatgccg cggctggcgg
     5581 agccacgcag ccgcgcgccg ccaggccggc gggcgcagga tgtcctcgag cgcggctcgc
     5641 acgtcggaca tgcggccagg ccgccgcgag gcgtccttct ccaggcagcg gtgaaggagg
     5701 gtccgaaccg cgcgcggagt gccggcggga agcgcgcccc agtcgggctc gcgctcgagg
     5761 accgcggtca tcgcgtcggc gtccgtgtcg cctgggaacg cgcgggcacc cgagagcagc
     5821 tcccaacaca ggcacccgaa cgcccagatg tcggcccggg catccacctc taagccgcgt
     5881 gcctgctcgg gactcatgta gcccggtgtg ccgacaatca gcccggcgag cgtttcgggc
     5941 gccgcaccag ccagcagcgc aggcgagtcc gcgcccgatg cgccggcctt ggccacgccg
     6001 aagtccagca ccttcacctt cctgtcgggc gtgaccctga tgttggctgg cttcaggtcg
     6061 cggtgaatga ttccgtgcgc gtgcgcggct tcgagcgcgc tcgcgacctg gcacgcgtag
     6121 cggagggccg tgtcagcgtc gagcggccct cggagcgcct cgccctccac cagctccagc
     6181 acgaggtagt cgaggccgtc cgattcctcg aggccgtaga tcgcggcgat gccggggtgg
     6241 ttgagggtcg ccagcgttcg ggcttcgcga tggatgcgcg ccagatggcc ggcgtcgcgc
     6301 gcgaactcgg gcggcagcag cttgatggcg actgcgcccg agcttgtcgt cgtgggcgcg
     6361 gtacacctcg cccatcccgc cggcgccgat gaacgactgg aggcggtagc tccccagttg
     6421 gcgtccgacg agcgagccgc gtaccgcagt gccggcctcg gccgggacga gcggcgagca
     6481 ttcaaggaac gagcccgccc gctcgtcgct cgccaggagg aggccgacct gcccgcgcag
     6541 ggcggcctcg cccgcacaag cctcgtccag gaacgccgcg cggtccttcg ccggccgcgc
     6601 aagcgccgcg tgaaagagct cctcgactcg ccgccagcca tcggcgtcga tcgccatcgg
     6661 ccaaggctcc gcgccagatt atagagggaa ggcttggtgc ctgcgtcaag gcgcgggact
     6721 tccctcggac gtcatggcct ggcgccgtgg cagaggctca ccacttgtcg tcgggccttc
     6781 cgctgcgaac ctgctgcgcg gtcatggtca tgatcggcga cagcggggtc ccgttgggca
     6841 gcgtcatcga cgtcatgatg ccacgcagct cgaagccgct gtccaccagg acgaacgtcc
     6901 agacagacag gcgctggggc accccgtccc ggtaggcggt gagcgtcaat gtccccgtgc
     6961 agtccggata gacctcgtag gtcccttctt tcgtgtcggc accgaccgcc ccgttggcgc
     7021 tgctgttctg tttgccgtcg aagtgcccgg cgccgtcgaa cccgtagcgg ccgaccgcgg
     7081 ccgcggtgac cgtgaccgtg ccgccggtcg gcgtgggggc aatcacccag cccgtctcgg
     7141 tgtagcccca tacccctctc agggtcttgt tcgagcaagc cgggtggctc tgggcggcgg
     7201 ccgtcccggc caacccgacg acgatggcga ctgcccaggc gattctgacg ctgtgacgct
     7261 tcatcgcgtg cacctcctga tccctattcc gcagactggc gaaggaaggg gtcatgggcg
     7321 gcgcgagcgg cccgcgcgcc tgtcggctgg cagcgccgcc aggcactcgc cggtcgtcgc
     7381 cgcggtgacc ccgggcatgg gggccgcggc gttggccgag ggtcagtggc ggtggtagat
     7441 cttgccgacg atctcccatc cgtcggggga ctcgtacagc cacaggtagt cggtgaagat
     7501 ccgtctgcgc tccggtatgg gcgtcgccgc tcgcgggcac gccggcagtc gaacacgggg
     7561 tgccgcgcag gaggaccggt gacatcggca gcgtggcaac tcgtctggat gcttctctga
     7621 ctcgattcga gcaggcgcct ggcgcgcggc ccccgcctcc ttcgatgccc gcacggggtc
     7681 ctcagagagc gagaacggat atagtatcca cgcaagtccg tcgcggcggc ctgggcgcgg
     7741 cgcgtgcggc gagttgccgg gaagccgggc agggggagct cgaggcgagc ttggaccagg
     7801 tctcagaacc gcggcgcaca agaaaggtcg gtcccatgct gatccgacgc gctctgagag
     7861 ccgcactccg cgtgttgttt cggccgggtc gcctcctgcc ggtcctcctg gccgcgctgc
     7921 cgttggccgt ggtcagcggc ggtcaggacc tcggccagct gaaaagaagg ctcaccggcg
     7981 tcgcggagaa cgtgacgctc cgccagcttc tggtggggcg acccctcacg accacgatcg
     8041 acgacgcggt ctatgacgtc ccctttctgg atccttacga tccgcccgac agccaggaca
     8101 tcacagtcct ccctcaagcg cctgacggcg gccggctgtt gcgtccgggc tcgttcgtgt
     8161 acctcgcccg gtcgtattgc gtcggcgtgg gcgcgcgccg gccctggcgc ggcgacggct
     8221 acgcgtacgc gccgatgaag ggtccgcagg ccgactcgat ccgctcgatc atccgcaact
     8281 cggcccgctt ccccgacatc cctcaggagg agatccagaa gcttctctgg gggatcatcg
     8341 ccagatcgag ctggagggac ctttctgccg aacagaaggc aatcgccgcc cgacttctga
     8401 gctcccggca gatcgcgctc ctcaacggaa gcgccctcga gcttcttccc ggcagtgtcc
     8461 tcgaccggtc gctagccggg gcttcgccgg ccgtggcgcg catccttggc gccgaggccc
     8521 gaatgcggga gatcttcgcc tccggcgtgg ccacgtacga ggagctggaa gaagtcgctg
     8581 tttcggcg
//
LOCUS       DAOSIF010000070         8457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7832_length_8457_cov_4.440371, whole genome shotgun sequence.
ACCESSION   DAOSIF010000070 DAOSIF010000000
VERSION     DAOSIF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7832_length_8457_cov_4.440371"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLT35_03905"
     CDS             <1..744
                     /locus_tag="PLT35_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28400.1"
                     /translation="HPEVRRRAGFLPEAPYFYDYLTAEELLSYFAGLFGLRDGARRAR
                     VAALLDLVGIGGERRLALRKCSKGMLQRVGLAQALLNEPDLVFLDEPMSGLDPLGRRD
                     VRAIVLGLRDRGCTVVFSSHILSDAEALCSRVGILVGGRLVAAGRLSDMLAFEVEGWE
                     LVMAGLDEAALGALGSRVRRATPLGGGRYAIELPPVDDPDCAVAGLRARGGRLVSLNP
                     MRATLEDYFVRQVEARAGGPTAGRTVPER"
     gene            741..1556
                     /locus_tag="PLT35_03910"
     CDS             741..1556
                     /locus_tag="PLT35_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG28401.1"
                     /translation="MMARVVGLVAYHVFKESVRDKVLYNLVLFAVLLIGGSYLVGQLT
                     AGQDIKIIKDLGLAASSLFGLFIAVFIGVGLVWKEVDRRSVYNLLVKPVRRHEFILGK
                     FLGLALTLLVNIAVMAAALYAVLALMSWLRPEIGRAGGVEAPVLDPRLLKAFGLLYAQ
                     LLVVTSVALFFSTFAGPLMSAAFTFGLYVVGHFNEDLAHFEDVVQSPAAGWLGKALYY
                     LLPNLAPFDVKLAAVHGREVPNAYCALTAAYGLLYIAAMLALAVVIFQRRDFK"
     gene            1559..2509
                     /locus_tag="PLT35_03915"
     CDS             1559..2509
                     /locus_tag="PLT35_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG28402.1"
                     /translation="MAGGGTDDRLARVRAPILLGAALACWAGAVSLGAFRDARHALPP
                     VADDVLYLTGGAAAPRLALGFDAILADVYWIRSIQHFGSTRRAKAGADKSYGLLYPLL
                     DLTTTLDPHFSIAYRFGSIFLSEPYPNGPGRTDQAIALLERGLAADPKKWQYAHDAGF
                     VHYWWRGDYRAAAEWFERASRIDGAPWWLTSMAATTLAQGGDRRSSRHLWQQLYESAD
                     NDWVKSNARLRLAQLDALDQMDALAALVRRHTEARGRPPYSWRELAPFGLRGVPLDSS
                     GTPFELDATAPGGVRLSPGSSLSPLPPALAGQQGRAGQAP"
     gene            2506..3273
                     /locus_tag="PLT35_03920"
     CDS             2506..3273
                     /locus_tag="PLT35_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOG28403.1"
                     /translation="MTTAGLVLAGLLGACVGSFLNVCIYRLPLEQSLLVPASHCMACG
                     RKLSWFENVPVAAWIALRGRCRTCRARIPAVYPLVEAFTAAMFVWAAWQYGASWLLVS
                     RLLLGCALIVLFFIDLHHRILPNVITVYGTAVGFLLSFLAPPGWLSSLIGLVIGGLIP
                     LGVAEAYFRLRKIEGLGMGDVKMLALIGAFLGWQLVLLTLTVASVLGAIVGLGLIAIR
                     GGTLRHALPFGTFLAVAAMFAAACGEPIVRWYVGFYR"
     gene            3501..3977
                     /locus_tag="PLT35_03925"
     CDS             3501..3977
                     /locus_tag="PLT35_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28404.1"
                     /translation="MLLRHPGMKRIGCQSGTTLVEAVIAIGLLAGAVTALAGLAHSAI
                     RAATLARERSIEAVCALEKIEGLCRDVRLLAVSPDGTLTADVPGFVEYLDARGAVATR
                     ANRVFVRRWSVAPVRADPDLLAIQVAVAACRGAGSAGPCEAASGVRFASIRSRLAW"
     gene            3971..5149
                     /locus_tag="PLT35_03930"
     CDS             3971..5149
                     /locus_tag="PLT35_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG28405.1"
                     /translation="MVTARRAAGFTLVELLVAAAVTLAALGLAVLLLLPSAAAFHALP
                     EAVDAQQRLRVAVQTLADSIRGAGASPVLESATRGAPAWPAVLPCRLAGGPLAAVPGG
                     CVGSEAITTLTVALGAPQAALAEALTDPTAPIRLAPLSACALSAPPCRVRAGSQVLVE
                     DGTGAWDILSVSAVSADGALVEHAGSPLSRPYKAGATVAEVEAAAYSLRVEAATNVPV
                     LRRATGPSSDLPVVDHVAALRLEYFGAAEAPLVLDDADANLRRTTYGPLPPQAGLDDP
                     LDAWAAGENCLFSRSGGEPVARVASLPAERLGLARLPPALFADGPWCPDAGAPNRYDA
                     DLLRVRLVRIVLRIEAQSSSVRGLSPQLFSRPGTSRESARLVPDLEAHVDVAIRNGGR
                     "
     gene            5115..5891
                     /locus_tag="PLT35_03935"
     CDS             5115..5891
                     /locus_tag="PLT35_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28406.1"
                     /translation="MSTWRFETAAGERQGRTPAGRRRGGRCGLREERGVALVAALAVM
                     ALVAAIGLGLALTTSLEPLAAASYETSWSARLAAEAGLAAAAHELGAAGDWNAVLGGE
                     LASGVLGRAAVSVELPDGSRAGLQDLTNLATCGHAAACTDPEAAAFAVGRPWGPNNPR
                     WRVFGSARLDQVIEAGAGLPPIDIVVWTADDPAEADGDPLHDTPPGSTGPRPPGACTV
                     AVRAEAFAPRLGHAAVVGVVTRPGPACRPGARLAAWHAFH"
     gene            5974..6612
                     /locus_tag="PLT35_03940"
     CDS             5974..6612
                     /locus_tag="PLT35_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28407.1"
                     /translation="MDRGVRRQPRGWLVTRTNTLVARAAAAGVLAVAVCCASVPAFAQ
                     SLGEVARKQAERRKTITGPVTVITNDHLKPVPAVPAKAAGEAGQPDVPEAGAPPEAGA
                     PPETGAPEQPPPPPDPTKDQEHWRRRMTDAIQQRDRNRFLMEAVQSRINALTTDFYAR
                     DDPFQRAKIGEERQRTIEELARMKLEQEKLEQKIADIEEEARRANVPPGWLR"
     gene            6733..8193
                     /gene="proS"
                     /locus_tag="PLT35_03945"
     CDS             6733..8193
                     /gene="proS"
                     /locus_tag="PLT35_03945"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG28408.1"
                     /translation="MAEQDGRKEPLITEITPKSEDFSRWYTDLVRRAELADYSPVKGC
                     MVIRPYGYAIWEFIQRGLDSRIKATGHVNAYFPLFIPKSLLVKEAEHVEGFAPQVAWV
                     TRGGTEELEEPLVVRPTSEAIIGTMYAKWIQSWRDLPVLINQWANIVRWEKVTRLFLR
                     TTEFLWQEGHTAHETAEEAEAETLMILRLYKEFVETELAVPVIAGRKSASEQFAGAER
                     TYSIEALMGDGRALQAGTSHNLGQNFAKAFDIQFQARDKSLQYIWGTSWGMTTRLVGA
                     VIMVHGDESGLVLPPRIAPYQVVIVPIPRGNWRETVLPHAQQVRDTLTAAGVRVMLDD
                     RDAYTPGWKFSEWELRGVPLRLEVGPKDIERQQVVLARRDTREKIPTPVSGLPGRVQE
                     LLDAIQRNLFERALAFQREHTTRTSSWDEFTRTLEGRPGFVVSPWCGSEACEAEIKGL
                     TQATIRTLPMDAEPSGAPCVKCGQPSVADAWFAKAY"
     gene            complement(8228..>8457)
                     /locus_tag="PLT35_03950"
     CDS             complement(8228..>8457)
                     /locus_tag="PLT35_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28409.1"
                     /translation="LEALAERLARAAAPGAAPEPLADRLLAAGRAIVEREKLTRRHAS
                     IKKVARLSAAVADALVAENGPPAPDLLSPRG"
BASE COUNT         1077 a         3018 c         3110 g         1252 t
ORIGIN      
        1 caccccgagg tgcggcgccg ggccggcttc ctgcccgagg cgccgtactt ctacgactac
       61 ctcacggccg aggagctgct ctcgtacttc gccggcctct tcgggctgcg cgacggcgcc
      121 aggcgggcga gggtcgccgc gctgctcgac ctggtgggca tcggcggcga gcgccggctg
      181 gcgctgcgaa agtgctcgaa gggcatgctc cagcgcgtcg gcctcgccca ggcgctgctg
      241 aacgagcccg acctcgtgtt cctcgacgag ccgatgtcgg gcctcgatcc gctcgggcgg
      301 cgggacgtgc gcgcgatcgt cctcggcctg cgggaccgcg gctgcacggt ggtgttcagc
      361 tcgcacatcc tctcggacgc cgaggcgctc tgcagccgcg tcggcatcct ggtcggcggc
      421 cggctggtcg ccgcgggccg gctctcggac atgctggcgt tcgaggtgga gggctgggag
      481 ctcgtcatgg cgggcctcga cgaggcggcg ctcggggcgc tcggcagccg cgtccgccgc
      541 gccacgccgc tcggcggcgg gcgctacgcc atcgagctgc cgccggtcga cgaccccgac
      601 tgcgccgtgg ccggcctgcg ggcgcggggc ggccgcctgg tgtcgctcaa cccgatgcgg
      661 gcgaccctcg aggactactt cgtgcggcag gtcgaggcgc gcgcgggcgg gccgacggcc
      721 gggcgcacgg tgccggagcg atgatggcgc gcgtcgtcgg cctcgtcgcg taccacgtgt
      781 tcaaggagtc cgtacgggac aaggtcctgt acaacctggt gctcttcgcg gtgctcctca
      841 tcggcgggtc gtacctggtc ggccagctca cggccgggca ggacatcaag atcatcaagg
      901 acctcggcct ggcggcctcg tcgctcttcg ggctgttcat cgcggtgttc atcggcgtcg
      961 gcctggtctg gaaggaagtc gatcgccgca gcgtctacaa cctgctcgtc aagcccgtcc
     1021 gccgccacga gttcatcctc ggcaagttcc tcggcctggc gctcacgctg ctggtcaaca
     1081 tcgccgtgat ggcggcggcg ctgtacgcgg tgctcgcgct gatgagctgg cttcggccgg
     1141 agatcggccg cgccggcggc gtcgaggcgc ccgtgctcga cccgcgcctg ctgaaggcgt
     1201 tcggcctgct ctacgcgcag ttgctggtcg tcacctcggt ggcgctcttc ttctccacgt
     1261 tcgcgggccc gctgatgtcc gccgccttca cgttcggcct ctacgtcgtc ggccacttca
     1321 acgaggacct ggcgcacttc gaggacgtcg tgcagtcgcc cgcggccgga tggctcggca
     1381 aggcgctcta ctacctgctg ccgaacctgg cgcccttcga cgtgaagctc gcggcggtgc
     1441 acggccgcga ggtgccgaac gcgtactgcg cgctcacggc ggcctacggc ctcctgtaca
     1501 tcgcggcgat gctggcgctc gccgtcgtga tcttccagcg ccgggacttc aagtagccat
     1561 ggcgggcggc ggcacggacg atcgcctggc gcgggtgcgc gcgccgatcc tgctcggggc
     1621 ggcgctcgcc tgctgggccg gcgcggtctc gctcggcgcg ttccgggacg cccgccacgc
     1681 cctgccgccg gtggccgacg acgtgctgta cctcacgggc ggggccgccg cgccgcgcct
     1741 ggcgctcggg ttcgacgcga tcctcgccga cgtttactgg atccgctcga tccagcactt
     1801 cggcagcacg aggcgggcga aggccggggc cgacaagtcc tacggcctgc tctacccgct
     1861 gctcgacctg acgacgacgc tcgacccgca tttctccatc gcgtaccggt tcggctcgat
     1921 cttcctgtcc gagccctacc cgaacgggcc cggccgcacg gaccaggcca ttgccctgct
     1981 ggagcgcggg ctggccgccg atccgaagaa atggcagtac gcgcacgacg cgggcttcgt
     2041 ccactactgg tggcgagggg actacagggc agccgcggag tggttcgagc gcgcctcgcg
     2101 catcgacggc gcgccgtggt ggctcacgtc catggccgcc acgacgctgg cccagggcgg
     2161 cgaccggcgc tcgtcgcgcc atctgtggca gcagctctac gagagcgcgg acaacgactg
     2221 ggtgaagtcg aacgccaggc tgcggctcgc gcagctcgac gcgctcgacc agatggacgc
     2281 cctcgccgcg ctcgtccgcc ggcacaccga ggcgcgcggc aggcccccgt actcctggcg
     2341 ggagctcgcg ccgttcggcc tgcgcggtgt cccgctcgat tcctcaggga cgccctttga
     2401 gcttgatgcg acggccccgg gaggcgtccg gctgtcaccc gggtcgtcgc tttcgcccct
     2461 gccgccggcg ctcgccggtc agcaggggag ggcaggccag gcgccatgac caccgccgga
     2521 ctcgtcctcg caggcctgct cggcgcgtgt gtcgggagct tcctgaacgt ctgcatctac
     2581 cggctccccc tcgagcagtc gcttctcgtc cccgcctcgc actgcatggc gtgcggccgg
     2641 aaactcagct ggttcgagaa cgtccccgtt gccgcctgga tcgcgcttcg agggcgctgc
     2701 cgcacgtgcc gcgcgcggat cccggccgtg tatccgctgg tcgaggcctt caccgccgcg
     2761 atgttcgtct gggcggcgtg gcagtacggg gcgagctggc tgctcgtctc gcggctgctg
     2821 ctggggtgcg ccttgatcgt gctcttcttc atcgacctgc accaccggat cctcccgaac
     2881 gtcatcaccg tttacggcac cgccgtcggg ttcctcctga gcttcctggc gccgccgggc
     2941 tggctgtcgt cgctcatcgg cctcgtcatc ggcgggctca tcccgctcgg cgtggccgag
     3001 gcctacttcc ggcttcggaa gatcgaaggc ctcggcatgg gcgacgtgaa gatgctcgcg
     3061 ctcatcggcg cgttcctcgg ctggcagctg gtgctgctga cgctcaccgt cgcctccgtg
     3121 ctcggcgcga tcgtcggcct cggcctcatc gcgatcagag gcggcacgct caggcacgcg
     3181 ctgccgttcg gcacgttcct ggccgttgcc gccatgttcg ccgccgcctg cggggagccg
     3241 atcgtgcggt ggtacgtggg gttctatcga tagaatccgg aagccggaag tcggaagcca
     3301 gaaaccagaa gccggaagtc agaattcaga atgagagacg gagacaggcg gcaaagaaga
     3361 tagaggaggc ggagcgcaga tactgccgcg gtgggacgcg gactgcagtg caggttctgc
     3421 gacgcccgac acggcctttc gcgaaccgcc ggtaatcgcc ccggaaacca gccacttcga
     3481 gtcgagcgtt ttcggcaccg gtgttgctgc ggcacccggg catgaagcgc atcggctgcc
     3541 agtccggcac gacgctcgtc gaagccgtca tcgccatcgg cctgctggcc ggtgcggtga
     3601 cggcgctggc cgggctcgct cattccgcca tccgcgccgc gacgctggcg cgggaacgat
     3661 cgattgaagc cgtctgcgcc ctcgagaaga tcgaggggct ctgccgggac gtgcgcctgc
     3721 tggccgtctc gccggacggc acactgacgg ccgacgtgcc gggcttcgtg gagtacctcg
     3781 acgcgcgcgg cgccgtcgcc acgagggcca accgggtgtt cgtccgccgc tggtcggttg
     3841 cgccggtgcg cgcagatccg gacctgctgg cgatccaggt tgccgtcgcc gcctgccgcg
     3901 gggcgggctc ggcgggaccg tgcgaggcgg cttccggcgt gcgtttcgcc agcatccggt
     3961 cgaggctggc gtggtgacgg cgcgccgggc agccgggttc acgctggtcg agctgctggt
     4021 ggcggcggcg gtcacgctgg cggccctggg gctggccgtg ctgctgctcc tcccctcggc
     4081 cgcggcgttc cacgccctgc cggaggccgt tgacgcccag cagcgcctcc gggtcgccgt
     4141 gcagacgctg gccgacagca tccgcggggc tggcgcctcg ccggtcctcg aaagcgcgac
     4201 ccgcggtgcg ccggcgtggc ccgcggtgtt gccgtgccgc ctcgcgggag ggccgctggc
     4261 cgccgtaccc ggcggctgcg tcggcagcga ggcgatcacg acgctcaccg tggcgctcgg
     4321 cgcgccgcag gcggccctgg cagaggcgct caccgacccg acggcgccca tccggctggc
     4381 gccgctgtct gcctgcgcgt tgtcggcccc gccctgccgc gtccgcgccg gctcgcaggt
     4441 ccttgtcgag gacggcacgg gcgcgtggga catcctgtcc gtctcggcgg tctcggccga
     4501 cggcgcgctc gtcgagcacg ccggatcgcc gctgtcccgc ccgtacaagg ccggcgcgac
     4561 cgtcgctgag gtcgaggccg ccgcctacag cctccgcgtc gaggctgcca cgaacgttcc
     4621 tgtgctccgc cgtgcgaccg gcccgtcgtc ggacctgccg gtggtggacc acgtcgcggc
     4681 cttgcggctg gagtacttcg gggccgccga ggcgccgctc gtgctggacg acgctgacgc
     4741 gaacctccgc cgcacgacct acggcccgct gccgccccag gcgggcctgg acgatccgct
     4801 cgatgcctgg gccgccggcg aaaactgtct cttctcccgc agcggcggcg agccggtggc
     4861 gcgcgtggcc tcgctgcccg ccgagcgtct cggcctcgcg aggctgccgc cggccctgtt
     4921 cgccgacggt ccctggtgtc ccgacgcggg agcgcccaac cggtacgatg cggacctgct
     4981 gcgcgtccgg ctcgtccgca tcgtcctccg gatcgaggcg cagtcgtcgt cggtccgcgg
     5041 cctgagcccg cagttgttca gccggcccgg cacgtcgcgg gagagcgcgc ggctcgtgcc
     5101 ggacctggag gcccatgtcg acgtggcgat tcgaaacggc ggccggtgag cggcagggcc
     5161 ggacgccggc gggacggcgg cgaggcggga gatgcggcct gcgggaagag cggggcgttg
     5221 cgctcgtcgc ggcgctggct gtcatggcgc tcgttgcggc gattgggctc gggctggccc
     5281 tgacgacgag cctcgagccg ctggctgcgg cgagttacga gacgtcgtgg tccgcccgcc
     5341 tggctgccga ggccggtctg gcggctgccg cgcacgagct cggggccgcg ggagactgga
     5401 acgccgtcct cggaggagag ctggcctcgg gcgtcctcgg ccgggcggcc gtcagcgtcg
     5461 agctgccgga cggctcgcgc gccggcttgc aggacctgac gaacctcgcg acgtgcggcc
     5521 acgccgccgc gtgcacggac ccggaggcgg cggcgttcgc cgtgggccgt ccgtggggcc
     5581 cgaacaaccc gcggtggcgc gtgttcggga gcgcgcgtct cgaccaggtg atcgaggcag
     5641 gcgccggcct gcctcccatt gacatcgtgg tgtggacggc ggatgacccg gccgaggcgg
     5701 acggcgaccc gctccacgac accccgccgg ggtcgacggg accccgcccg cccggcgcgt
     5761 gcaccgtcgc ggtcagggcc gaggcgttcg cgccgcgcct tgggcatgcg gcggtggtgg
     5821 gcgttgtgac gcggccgggc cccgcgtgca ggcctggcgc ccgactggcg gcgtggcacg
     5881 cgttccactg actccactga ccgggtccgc cgaggcccta tcgaaaccgc ctcgccgggg
     5941 ctatagtcat gaggggcggc tgccgatacg acgatggacc ggggagtgcg ccggcagcca
     6001 agggggtggc tggtgaccag gacgaacacg ctcgttgccc gtgccgcggc cgctggagtg
     6061 ctggcggtgg ccgtctgctg cgcctccgtg ccggccttcg cccagtcgct cggcgaagtg
     6121 gcccggaaac aagccgagcg ccggaagacg atcaccgggc cggtcaccgt gatcacgaac
     6181 gaccacctca agcccgttcc cgccgtgccg gccaaggcgg ccggcgaggc cggtcagccc
     6241 gatgttccag aggctggcgc gccgcccgaa gctggcgcgc cgcccgagac gggtgctccg
     6301 gagcagccgc cgccgccgcc cgatcccacg aaggaccagg agcactggcg ccggcgcatg
     6361 acggacgcga tccagcagcg cgatcggaac cgcttcctga tggaggccgt gcagagccgc
     6421 atcaacgccc tcaccaccga cttctacgcg cgtgacgatc ccttccagcg cgccaagatc
     6481 ggcgaggagc ggcagcggac gatcgaggag ctggcgcgca tgaagctcga gcaggaaaag
     6541 ctggagcaga agatcgccga catcgaggaa gaagcccgcc gcgccaacgt cccgcccggc
     6601 tggctccggt aacgggcccg cttcgcgccc gatcgcggcc cccgcctccc gcgccgcaac
     6661 cgcgcctgcc ctggcgccac gccgcgccgt aggccgaccg gcgcctgccg cgtcgatggt
     6721 aggatacggg acatggcaga gcaggacggc cgcaaagagc ctctcatcac agaaatcacg
     6781 ccgaagtcgg aggacttctc gcgctggtac acggacctgg ttcgccgcgc cgaactggcg
     6841 gactattcgc cggtcaaggg ctgcatggtg atccggccct acgggtacgc gatctgggag
     6901 ttcatccagc gcgggctcga cagccggatc aaggcgaccg ggcatgtcaa cgcgtacttc
     6961 ccgctgttca tcccgaagag cctgctggtc aaggaggcgg agcacgtcga ggggttcgcg
     7021 ccgcaggtgg cgtgggtcac caggggcggc accgaagagc tcgaggagcc gctggtcgtc
     7081 cgcccgacgt cggaggccat catcggcacg atgtacgcca agtggatcca gtcctggcgc
     7141 gacctcccgg tgctcatcaa ccagtgggcg aacatcgtcc ggtgggagaa ggtgacgcgg
     7201 ctgttcctgc ggacgacgga gttcctctgg caggaggggc acaccgccca cgagaccgcc
     7261 gaggaggcgg aggcggagac gctcatgatc ctgcgcctct acaaggagtt cgtcgagacc
     7321 gagctggccg tgcctgtcat cgccggccgg aagtcggcga gcgagcagtt cgcgggcgcc
     7381 gagcggacct actccatcga ggcgctgatg ggcgacgggc gcgccctgca ggcgggcacg
     7441 tcgcacaacc tcggccagaa cttcgcgaag gcgttcgaca tccagttcca ggcgcgcgac
     7501 aagtcgctgc agtacatctg gggcacgtcc tggggcatga cgacgcggct cgtcggggcg
     7561 gtcatcatgg tgcacggcga cgagtccggc ctggtgctgc cgccccggat cgcgccgtac
     7621 caggtcgtca tcgtgccgat cccgcggggc aactggcgcg agaccgtgct tccgcacgcg
     7681 cagcaggtcc gcgacacgct gacggccgcc ggcgtgcgcg tgatgctcga cgaccgggac
     7741 gcctacacgc cgggctggaa gttctcggag tgggagcttc ggggcgtgcc tctgcggctc
     7801 gaggtgggcc cgaaggacat cgagcggcag caggtcgtgc tcgcgcgccg cgacacccgc
     7861 gagaagatcc cgacgcccgt ctcgggcctg cccggccgcg tccaggagct gctcgacgcc
     7921 atccagcgga acctgttcga gcgcgcgctc gcgttccagc gcgagcacac gacccggacc
     7981 tcgtcgtggg acgagttcac ccggacgctc gaagggcggc cgggtttcgt ggtgtcgccc
     8041 tggtgcggca gcgaggcgtg cgaggccgag atcaagggcc tgacgcaggc gacgatccgc
     8101 acgctgccga tggacgcgga gccgagcggc gcgccctgcg tgaagtgcgg gcagccgtcg
     8161 gtcgcggacg cctggttcgc gaaggcgtac taacggaatc cggaacgcag aagggggcct
     8221 cgggccttca gccccggggg ctcagcagat ccggggccgg aggcccgttc tcggcgacga
     8281 gcgcgtcggc gacggcggcc gacaatcgcg cgaccttctt gatggaggcg tgccgtctgg
     8341 tcagcttttc gcgctcgacg atcgcccggc cggccgcgag cagccggtca gcgaggggct
     8401 cgggggcggc gcccggagcg gcggcccggg cgaggcgctc tgcgagcgct tccaggc
//
LOCUS       DAOSIF010000071         8447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7844_length_8447_cov_4.025262, whole genome shotgun sequence.
ACCESSION   DAOSIF010000071 DAOSIF010000000
VERSION     DAOSIF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7844_length_8447_cov_4.025262"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLT35_03955"
     CDS             complement(<1..885)
                     /locus_tag="PLT35_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOG28410.1"
                     /translation="MMTRLLRLITAAAFPAALALAVTAGMSAQGRPSPATTQAPSAAA
                     AVAQDWAARPSSERTYTVKLDFNRWHDVSELQADMRTLEKAYPKFLKYISLGKSYGGR
                     DIAAMIVNNPDTGPDTAKPAMYIEANIHGNEVQGGEICLYTIWYLMEHYGRIDRITRL
                     VDERAFYIVPTVNPDGRDYFMHGPGGNARSGHVPVDDDNDYLFDEDGPEDLNGNGVIE
                     QIRKYVPGEGTHRKSSADPRILEPVPPGETGDYVLLGLEGIDHDGDGRVGEDGPGGYD
                     PNRNFPADWEQVDYTYGLV"
     gene            complement(913..2934)
                     /locus_tag="PLT35_03960"
     CDS             complement(913..2934)
                     /locus_tag="PLT35_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOG28411.1"
                     /translation="MRTLALRVGAAVLVLAASSAAAGRTDPPPQAAPAGAPAYHDYAA
                     LSAALKAAVAAHPGLAKLASIGKTRQGRDIWAVEVASAKGRPVGERPALLVAANFEGD
                     HLIGSEIALCMIDYLLTAYASDAEVRQRLDASAIYVLPRVNPDGAELMFAPVKAFRRT
                     NATPVDADNDGRADEDGPEDLNGDGVVTVMRVRDPKGPYAVSAEDARLMKRADPAKGE
                     AGGWAIYPEGIDNDGDGFINEDGPGGVDINRNFMHQYPYFEPDAGRYMASEAETRAVL
                     DYVLAHRNIAAILAFGESDNLIAGPARPAPATRLDLVEFAERSFADARRTGMVPPLGA
                     GFGRGFGRAGAMMPGAAPAGGAGGRSAQGAAPAASGRGMRTVQPATSVNAADLEYLNT
                     IAAKYREMTGLRATGLVRAPAGAFFEYGYYQYGVPSFSTPGWGLPGGARPSAGAVPPG
                     AAPGRAGASPAAGQAGPPAAFGQRGAGRGSTGPAGPGSDAEAAAEAIDLRLLQWMDAE
                     KIDGFVPWTPFKHPTLGDVEIGGFKPYAAVNPPASMIADLGRAHAKFSVYLASLFANL
                     RIAKAEATALGGGLFRIKADVENTGFLPTALAHGVVSRAVKPVMVRLDVPPDSIVTGA
                     EKISALPTLAGSGSRESYEWVIAAAPGSSVTLTVMSQKGGSDRATIALR"
     gene            3147..3803
                     /locus_tag="PLT35_03965"
     CDS             3147..3803
                     /locus_tag="PLT35_03965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG28412.1"
                     /translation="MTRLPVLMLAAALCGPALVAIDAQSPPAAGGLDARVERFFAEHR
                     YDWRDMNVPESDGRLLHDLVVKGQYTRALEIGTSTGRSGIWIALALSKTGGKLTTIDI
                     DRRRHEQAVRNFQEAGVSAFIDARLADAHDLVPALPGPFDFVFIDADKDWYTNYAKAV
                     IPKLTVGGCIAAHNVYPARRWRGGGMTGDYYEYMSGLPFLETTVTPGGVAISYKRRER
                     "
     gene            3813..5582
                     /locus_tag="PLT35_03970"
     CDS             3813..5582
                     /locus_tag="PLT35_03970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG28413.1"
                     /translation="MLLRAALLCGAVVLAVFLPPEARAPAAAQDEPRAVGEIGRALDE
                     YLSRLERFGFAGGAAAVRGNQVVLLKSYGLADRSRRVPLGTDSVFSIGSITKQFTAAA
                     VLTLEMQGRLAVGDAIGKYLDGVPPDKTGMTLHHLLTHSSGLESDFSSSDYDPVGREE
                     YVGRALASTLLFAPGRGYQYANAGYSLLAAIIERVSGEPYEAYLREHVLLPAGMRETG
                     YKIPAWPPARIAHGYLDTGEDWGTLAERLEPAGAPYWTLRGNGGLQTTLPDMVAWHRA
                     LGTDAVLSKAARAKYFAPYVAEGPRGLSHYAYGWVVSKSARGTTVVRHNGGNGVYVAE
                     FVRFPDEDAMLFLASTDAGWPATPAAEALERILFGGTAAMPPRAVSEPPERLAEYAGD
                     WRLPRGSTLTLALDGASLLARPSAPEAFAALSAPDPALAERLADVSRRTAAITEKAFA
                     GDVVLLHAAMGRSMPLEALRTREFEMMRDRERRLGPFRGSAVVGALPRGPNSARVFVR
                     LDFETASVYNVYVWGPARIIGLRGSPEPPAFRYLPIGGGSFASFSFDGGGTVRSLRFV
                     MRDGALRLLLGSGPAPAEAVKNQ"
     gene            complement(5633..6958)
                     /locus_tag="PLT35_03975"
     CDS             complement(5633..6958)
                     /locus_tag="PLT35_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28414.1"
                     /translation="MASSPPGELALSSLLWFWLPLAATWLMMSAEGPYVAAIIARMPD
                     AAFNLAAYGVAFSLAWLVESPIMMLLTASNSLVRDRQSFLALERFMIRMNVAVTACML
                     IGVAPPVFRFVTGTIMGLPPNVAGLVHLATAVLVPWPAAIGYRRFYQGLLVRRGLTRR
                     VAYGTVVRLGTMSVTAAVLALTSALHGAAIGAAALACGVLAEAAASRLMARRVVASLL
                     AEPAGPASAPLTQRAIVRFYYPLALTSIISLVTGPMLTFFLGRGRAPIESLAVMPVVQ
                     SLVFLFRSGGVAYQEVGVALLGARGEHEAAIARGARALAAGATAVLAGILFTPLADAW
                     FAGLSGLAPDLSRFALTPARLLLLLPAMEYWLSFQRSRFILNGKTRVITVATAVEVGA
                     IALLMTAGIGLLGLVGVVAAATAQVLGRLGSNLFLRAASKPPPGAERAS"
     gene            complement(7005..8369)
                     /locus_tag="PLT35_03980"
     CDS             complement(7005..8369)
                     /locus_tag="PLT35_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG28415.1"
                     /translation="MSRSHQPTRRRFIGTLAGSAAAAAAVTMSAASREREEVIPDREK
                     GYAVNDQIQFALIGAGIQGVADTTSALQVPGVKLVAVCDLYDGRLTSAKARWGQDLFT
                     TRDYREIISRPDIDAVIAAVPDHWHKQIAIDTLGQNKSIYLEKPMVQSLGEGEAVIAA
                     QKTSKGILQVGSQGMSSLSNEKARELLAAGAIGKLTYAEGFWARNSPSGAWQYPIPAD
                     ASEQTLDWDKFLGATAKRPYDPLRFFRWRNYRDYGTGVCGDLFVHLFSTLHFITGSLG
                     PTQIMAQGGLRFWKDGRDVPDVMLGMFDYPETKEHAPFSLSLRVHFVDGTSGSTFLRL
                     VGTGGAMDVGFENEVTVKYQKTWDPLKATQGSFRANPEPTDRKDMVPAANIVYRAEQG
                     YKGAHYDHFNNWFTAIRTGGKVVEDAVFGYRAAAPALACNLSYFEKRAIQWDPVAMKL
                     VPGK"
BASE COUNT         1210 a         2955 c         3037 g         1245 t
ORIGIN      
        1 gaccaggcca taagtgtagt ccacctgctc ccagtcggcc gggaagttgc ggttgggatc
       61 gtagccgccg ggcccgtcct cccccacgcg gccgtcgccg tcgtggtcga tgccctccag
      121 cccgagcagc acgtagtcgc ccgtctcgcc gggcgggacc ggctcgagga tgcgcggatc
      181 ggcggagctc ttccggtgcg tgccttcgcc gggcacgtac ttccggatct gctcgatgac
      241 gccgttgccg ttcaggtcct cggggccgtc ctcgtcgaag aggtagtcgt tgtcgtcgtc
      301 caccggcacg tgtccgctcc gggcgttgcc ccccggcccg tgcatgaagt agtcgcggcc
      361 gtcgggattg accgtcggga cgatgtagaa ggcgcgctcg tcgacgaggc gggtgatgcg
      421 gtcgatcctg ccgtagtgct ccatcaggta ccagatcgtg tagaggcaga tctcgccgcc
      481 ctggacctcg ttgccgtgga tgttcgcctc gatgtacatc gccggcttgg ccgtgtccgg
      541 accggtgtcg gggttgttga cgatcatcgc ggcgatgtcc cgcccgccgt agctcttgcc
      601 gagcgagatg tacttgagga acttcgggta ggccttctcg agggtgcgca tgtcggcctg
      661 cagctccgac acgtcgtgcc agcggttgaa gtcgagcttc accgtgtagg tccgctcgga
      721 cgaaggccgc gcggcccagt cctgcgcgac ggctgccgcg gcgctaggcg cctgcgtcgt
      781 cgccggcgag gggcggccct gggcggacat gcccgccgtc acggcgagcg ccagcgccgc
      841 aggaaaggcc gccgcggtga tgagtctgag gagtctggtc atcatcggtg ctccctggtg
      901 tggcgtcggc cgctaccgca gcgcgatcgt cgcgcgatcg ctgccgccct tctgtgacat
      961 gacggtcaga gtaaccgagg agcccggggc tgccgcgatc acccattcgt aggattcgcg
     1021 cgagccggac cccgcgagcg tcggcagggc gctgatcttc tcggcgcccg tgacaatcga
     1081 gtcaggcggc acgtcgagcc gcaccatgac cggcttcacc gcccgcgaga cgacgccgtg
     1141 cgccagggcc gtcggcagga agccggtgtt ctcgacgtcg gccttgatgc ggaagaggcc
     1201 ccctccgagg gccgtcgcct ccgccttcgc gatccggaga ttcgcaaaga gcgacgcaag
     1261 gtagaccgag aacttcgcgt gggccctgcc caggtcggcg atcatcgacg cggggggatt
     1321 cacggccgcg tacggcttga agccgccgat ttcgacgtcg ccgagcgtcg ggtgcttgaa
     1381 cggcgtccac ggcacgaagc cgtcgatctt ctcggcgtcc atccactgga ggaggcgcag
     1441 gtcgattgcc tcggcggccg cctcggcgtc gctgcctggc cccgccgggc ctgtgcttcc
     1501 ccggcccgcg ccgcgctggc cgaacgcagc cggcgggccg gcctgtccgg cggccggact
     1561 ggcgccggcc cgtccgggag cggcacccgg cggcaccgcg ccggccgagg gcctcgcccc
     1621 tcctggcagg ccccagcccg gcgtggagaa ggacggcacg ccgtactggt agtagccgta
     1681 ttcgaagaac gcgccggccg gcgcgcgcac gaggcccgtg gcgcgaagcc ccgtcatctc
     1741 ccggtacttc gcggcgatgg tgttgaggta ctcgaggtcc gcggcgttca ccgacgtcgc
     1801 gggctggacg gtgcgcatgc cgcggccgct tgctgccggc gccgcgccct gagccgagcg
     1861 cccgcctgct ccgccggctg gggcggcccc gggcatcatg gcgccagccc tgccgaagcc
     1921 cctgccgaag ccggcgccga gcggcgggac catgccggtc cgccgcgcgt cggcgaagct
     1981 gcgttcggcg aactccacga gatccagcct ggtcgcgggc gccggcctcg cgggccccgc
     2041 gatcaggttg tcgctctcgc cgaacgcgag gatggcagcg atgttccggt gcgcgagcac
     2101 gtagtcgagc acggcccgcg tctccgcctc gctcgccatg taccggccgg cgtcgggttc
     2161 gaagtacggg tactggtgca tgaagttccg gttgatgtcc acgcccccgg ggccgtcctc
     2221 gttgatgaag ccgtccccgt cgttgtcgat gccctcgggg tagatcgccc atccgccggc
     2281 ctcgcccttg gccgggtcgg cgcgcttcat gaggcgcgcg tcctcggcgc tgacggcata
     2341 gggccccttc ggatccctga cgcgcatcac ggtgacgacg ccgtccccgt tgaggtcctc
     2401 ggggccgtcc tcgtcggcgc gcccgtcgtt gtcggcatcg acgggcgtgg cgttcgtgcg
     2461 gcggaacgcc ttcaccgggg cgaacatcaa ctccgctccg tcggggttga cgcgcggcag
     2521 cacgtagatg gccgacgcgt ccaggcgctg cctgacctcg gcgtccgagg cgtacgccgt
     2581 gagcaggtag tcgatcatgc agagcgcgat ctcgctgccg atgaggtgat cgccctcgaa
     2641 gttcgcggcg accaggagcg ccgggcgctc gccaaccggc cggcccttgg cgctcgcgac
     2701 ttccaccgcc cagatgtcgc ggccctgcct cgtcttgccg atcgacgcga gcttcgcgag
     2761 accggggtgg gcggcgacgg cggccttgag cgcggcggag agcgcagcgt agtcgtggta
     2821 ggcgggagcg ccagccggag cggcctgcgg cgggggatcg gttcgtcccg cggcggcgga
     2881 cgacgccgcc aggacgagga ccgccgcgcc aacacggagt gctagggtcc gcacgggtat
     2941 cctcctcgga aggcgatggg gccggcccgg acgcggccgg agcccgaatg cagagaaggc
     3001 tatcacagcg gccgcccgtg cccggccgcg aactcgcggg cggcccgcgg gtaaacgcag
     3061 gccggcgccc gtcgtaatgt ctcttcagtg gcgccggtcc gtgcgccggc gatggccggt
     3121 ggcctgctgt ctgccggagg tcctcgatga cccgtctgcc cgtcctgatg ctcgcggctg
     3181 cactctgcgg cccggccctc gtcgccatcg acgcccagag cccgccggcg gctggcgggc
     3241 tcgacgcgcg cgtcgagcgc tttttcgcag agcaccgtta cgactggcgg gacatgaacg
     3301 tgcccgagtc cgacgggcgt ttgctccacg atctcgtcgt caagggccaa tatacgcgcg
     3361 cccttgagat cggcacctcg actggccggt cgggcatctg gatcgccctc gccctctcga
     3421 agaccggcgg gaagctgacg acgatcgaca tcgatcgcag gcgccacgag caggccgtcc
     3481 gcaacttcca ggaggccggc gtgtcggcgt tcatcgacgc gcggctggcc gacgcgcacg
     3541 atctcgtgcc ggcgcttccc gggcccttcg acttcgtgtt catcgacgcc gacaaggact
     3601 ggtacacgaa ctacgcgaaa gccgtgatcc cgaagctgac ggtcggggga tgcatcgccg
     3661 cccacaacgt gtaccccgca cgccgctggc gcggcggcgg catgacgggc gactactacg
     3721 aatacatgtc cggcctgccg ttcctggaga cgacggtgac gccgggcggc gtggccatca
     3781 gctacaagcg ccgggagcgg tagaatcccc cgatgctgct gcgtgccgcc ctcttgtgcg
     3841 gcgccgtcgt cctggccgtg ttcctgccgc ctgaggcgcg cgctccggcg gccgcccagg
     3901 acgagccgcg cgcagtaggc gagatcggcc gcgccctcga tgagtatctc tcccgcctgg
     3961 agcggttcgg tttcgccggc ggcgccgcgg ccgtccgggg caatcaggtc gtgctgctca
     4021 agtcctacgg cctggcggac cgatcgcgcc gggtcccgct cggcaccgac agcgtcttca
     4081 gcatcggatc gatcaccaag cagttcaccg ccgcggccgt cctgacgctc gagatgcagg
     4141 gcagactggc cgtcggcgac gccatcggga agtacctcga cggggtcccg ccggacaaaa
     4201 ccggcatgac gctgcaccac ctgctgaccc actcgtccgg gctcgagtcg gatttcagtt
     4261 cgagcgacta cgatccagtc ggccgcgagg agtacgtcgg ccgcgcgctg gcgtccacgc
     4321 tgctgttcgc gcccggcaga ggctatcagt acgcgaacgc cgggtacagc ctgctggctg
     4381 ccatcatcga gcgggtgtcg ggggagccgt acgaggccta cctccgggag cacgtactcc
     4441 ttccagcagg catgcgcgag accggctaca agatcccggc ctggcccccg gcgcggatcg
     4501 ctcatggcta tctcgacacc ggagaagact gggggacgct cgccgagcgc cttgagcccg
     4561 ccggcgcgcc atactggacg ctgcggggga atggggggct gcagacgacc ctgcccgaca
     4621 tggtcgcctg gcaccgcgcc ctcggcacgg acgccgtgct gtcgaaggcg gcccgcgcga
     4681 agtacttcgc gccctacgtc gccgaggggc cccgcggact gtcgcactat gcgtacggct
     4741 gggtcgtcag caagagcgcc cgcggcacca ccgtcgtccg gcacaacggc gggaacggcg
     4801 tctacgtggc ggagttcgtg cggttccccg acgaggacgc catgctcttc ctggcctcga
     4861 ccgacgcggg ctggccggcg acgcccgctg ccgaggccct cgaacggatc ctgttcggcg
     4921 ggacggccgc gatgccgcca cgcgccgttt ccgagccgcc cgaacgcctt gcggagtacg
     4981 ccggcgactg gcgcctgccg agggggagca cgctgacact ggccctggac ggcgcgtcgc
     5041 tgctggcgcg gccgtcggcc ccggaggcgt tcgccgccct ctcggcgccg gacccggcgc
     5101 ttgccgagcg tctggcggat gtctcgcggc gcaccgccgc catcacggag aaggccttcg
     5161 ccggcgacgt cgtgctgctg cacgcggcga tggggcggag catgcccctg gaagcgctgc
     5221 ggacgcgcga gttcgagatg atgcgcgatc gcgaacgccg cctcggcccg ttcaggggat
     5281 cagcggtcgt cggggcgctg ccgcgcggtc ccaacagcgc acgcgtgttc gtccgcctcg
     5341 acttcgagac ggcaagcgtc tacaacgtgt acgtctgggg gccggcacgc atcatcggat
     5401 tgcgcgggtc gcccgagccg ccggcgttcc gctacctgcc gatcggcggc gggagcttcg
     5461 cgtccttcag tttcgacggc ggcgggaccg tgcggagtct ccgcttcgtc atgcgagacg
     5521 gagcgctccg gctgcttctc gggtccgggc cggcgcccgc cgaagctgtg aagaaccagt
     5581 aagggcgagg ccgagcgagc cgcgcggctg gcctggccgg aggagcccgg agctacgagg
     5641 cgcgctcggc gccggggggc ggcttcgagg cggcccggag aaacaggttc gagcccagcc
     5701 ggccgaggac ctgcgccgtc gccgccgcca ccacgccgac gagtccgagg agcccgatcc
     5761 ctgccgtcat gagcagcgcg atcgcgccga cctcgaccgc ggttgccacg gtgatcacgc
     5821 gcgtctttcc gttcaggatg aagcgggacc gctggaacga cagccagtac tccatcgccg
     5881 gaagcagcag gagcaggcgc gcgggcgtga gcgcgaaccg cgacagatcc ggcgccaggc
     5941 ccgacaggcc ggcgaaccag gcgtcggcga ggggcgtgaa caggatcccc gcaagaacgg
     6001 ccgtcgcgcc ggcggccaga gccctggcgc cgcgtgcgat cgcggcctcg tgttcgcctc
     6061 gcgcgcccag cagcgccacg cccacttcct ggtaggcgac gccgccgctc cggaacagga
     6121 acacgagcga ctgcaccacc ggcatgacgg cgagcgactc gatcggcgcc cggccccggc
     6181 ccaggaagaa cgtcagcatc gggccggtga cgagcgagat gatcgacgtc aaggcgagcg
     6241 ggtagtagaa gcgaacgatg gcgcgctgcg tgagcggggc cgaagccggg ccggccggct
     6301 cggcgaggag cgacgccaca acgcggcggg ccatcagacg gctggcggcc gcttccgcca
     6361 gcacgccgca ggccagagcg gcggcgccga tggcggcgcc gtggagcgcg gacgtcaggg
     6421 ccaggacggc cgccgtcacc gacatcgtcc cgaggcggac gacggtgccg taggccaccc
     6481 gccgcgtcag gccccggcgg acgagcaggc cctggtagaa gcggcggtac ccaatggccg
     6541 cgggccaggg aacgaggacg gccgtcgcca ggtggaccag tcccgcgacg ttcggcggca
     6601 ggcccatgat cgtgccggtc acgaagcgga agaccggcgg cgccacgccg atgagcatgc
     6661 atgccgtgac cgccacgttc atgcgaatca tgaaccgctc gagcgccagg aacgactgcc
     6721 ggtcgcgcac cagcgagttc gacgccgtca gcagcatcat gatgggggat tcgaccagcc
     6781 aggcgaggga gaacgccacg ccgtaggccg cgaggttgaa cgcggcgtcg ggcatgcggg
     6841 cgatgatggc ggcgacgtag ggcccctcgg ccgacatcat cagccacgtg gccgccaggg
     6901 gcagccagaa ccagagcaac gaagaaaggg ccagctcgcc gggaggcgag ctggccatgg
     6961 tggggcgcat cagcagagag cgtcggccgt cgatgcgcgc acgcctactt gccgggcacc
     7021 agcttcatcg caaccggatc ccactggatc gcgcgcttct cgaagtagct gaggttgcac
     7081 gcgagggccg gggccgccgc ccggtagccg aacaccgcgt cttcgacgac cttgccgccg
     7141 gtcctgatgg ccgtgaacca gttgttgaag tgatcgtagt gcgcgccctt gtagccctgc
     7201 tcggcgcggt acacgatgtt ggccgccggc accatatcct tgcggtcggt cggctccggg
     7261 ttcgcgcgga agctgccctg cgtcgccttg agcggatccc aggtcttctg gtacttcacc
     7321 gtcacttcgt tctcgaaccc cacgtccatc gcgccgcccg tgccgaccag gcggaggaac
     7381 gtgctgccag acgtgccgtc cacgaagtgg acgcgcagcg acaggctgaa gggcgcgtgc
     7441 tccttcgtct ccggatagtc gaacatgccg agcatgacgt cgggcacgtc gcggccgtcc
     7501 ttccagaacc ggagcccgcc ctgggccatg atctgcgtcg ggcccagcga gcccgtgatg
     7561 aagtgcagcg tcgagaacag gtgcacgaac agatccccgc agacgccggt gccgtagtcc
     7621 cggtagttgc gccaccggaa gaagcggagc gggtcgtacg ggcgcttggc cgttgcgccg
     7681 aggaacttgt cccagtcgag cgtctgctcg gaggcgtcgg cgggaatcgg gtactgccac
     7741 gcgcctgaag ggctgttgcg cgcccagaag ccctcggcgt aggtcagctt gccgatggcg
     7801 ccggccgcca gcagctcccg cgccttctcg ttgctgagcg acgacatccc ctggctgccg
     7861 acctggagga tgcccttcga ggtcttctgc gcggcgatga ccgcctcgcc ctcgcccagg
     7921 ctctggacca tcggcttctc gaggtagatc gacttgttct gcccgagggt gtcgatggcg
     7981 atctgcttgt gccagtgatc ggggaccgcg gcaatgacag cgtcgatgtc cggccggctg
     8041 atgatctcgc ggtagtcccg cgtcgtgaac agatcctgcc cccacctggc cttggcggag
     8101 gtcagccgtc cgtcgtacag gtcgcacacg gccacgagct tgacacccgg cacctggagg
     8161 gcgctcgtcg tgtcggccac gccctggatg ccggctccga tgagcgcgaa ctggatctgg
     8221 tcgttgacgg catatccctt ctcgcgatcg gggatgacct cctcgcgttc tcgggaagcg
     8281 gcagacatcg tgacggcggc cgccgcggcg gcgctgcccg cgagggtgcc gatgaaacgg
     8341 cggcgggtgg gctgatggct ccgactcatg ctgaaccctc caaatgacat cgatacgcgg
     8401 gactgacggg ggcgggaaac ggggccgggt cccgggccgc cgggaca
//
LOCUS       DAOSIF010000072         8432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7868_length_8432_cov_4.528710, whole genome shotgun sequence.
ACCESSION   DAOSIF010000072 DAOSIF010000000
VERSION     DAOSIF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7868_length_8432_cov_4.528710"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLT35_03985"
     CDS             <1..925
                     /locus_tag="PLT35_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28416.1"
                     /translation="PIGLAAVTLALVSVAVVNLFTKQVATIAGAGFTVTLYAAFTASE
                     RATARRQDAARKLDQFHLLPVPDIGLDVLAARPGGVLVPVRDPRTLAHLKWALARTDT
                     QKQDLVVMTVRPLQGPDTGYRDFAGERVFREYEQELFTRVVAVAEREGRPVKLLVVPS
                     SNVADAIARTAVSLQASDTVIGESATLSGAEMAQRIGEAWDRVPKSQPVRSRLVVFRL
                     DGPPEMFHLGPHPPALSQEDLDLIHRIWQDAVRQFGLGVHHRDVVAVALEQLERDLAG
                     DGRERALARIAERSGRPPAPPAESDELRPGT"
     gene            1115..2005
                     /locus_tag="PLT35_03990"
     CDS             1115..2005
                     /locus_tag="PLT35_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG28417.1"
                     /translation="MNMATRWRAAGIITASVLMFGLGASSQTQEPPLVRFGVITDLHY
                     ADIDPAGPRVYRDSVAKLEACVRTMNAERVAFLVELGDFKDQDTPPVESRTLSYLRTI
                     EAAMAGFKGPRYHVLGNHDVDSLSKAQFLGVVENTGVAPPATRYTFVSGGVRFVVLDA
                     CHKADGSDYDRGNYDWTDSNIDAPQLAWLSSTLAGSAEPVIVFVHQQLDGTGSYYVKN
                     APDVRRVLERSGKVLAVFQGHRHAGAFSEIDGIPYFTLAAAVEQPGAENGAYSIVEVG
                     RGGGIRVIGRQRATSRTTTR"
     gene            complement(2072..2635)
                     /locus_tag="PLT35_03995"
     CDS             complement(2072..2635)
                     /locus_tag="PLT35_03995"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOG28418.1"
                     /translation="MAGRHVVRLAAVAFAVLACAVPAADAQAVFETPGLITSAGQSSD
                     VAIIKVMLNTQLKLGLEYKPIAQPSDLAGMKALVVSVGASTKGLGAAGLDMPREIART
                     TALLKAAKSEGLRVLVLHTGGEARRGKTSNDLIQAVLPLADHVVVVAAGNKDKLFTTL
                     GEKHGVPVTEVAKLADAGGAVRALFKQ"
     gene            complement(2826..4475)
                     /gene="ggt"
                     /locus_tag="PLT35_04000"
     CDS             complement(2826..4475)
                     /gene="ggt"
                     /locus_tag="PLT35_04000"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOG28419.1"
                     /translation="MIRQAFLTGTLAAALIVAAPALPRTGAVTRSAPVTAKRGMVASA
                     HMLASQAGLEILKAGGNAVDAAVAAAFAIGVVEPNASGLGGEGMMVIYLADSKQAVAV
                     DYRSSAPAAIDFPGPIPDAGHAAVAVPGTVAGLAMALEKYGSMTLARVVAPAIRLAER
                     GFVVSPTLAGIVADNFEAIAKNEALAAILCPGGLPIESGATLKNPALGASLRTIAAGG
                     AAAFYRGALADAIAADMAASGGRITRADLAAYRAVERAPVQGTYRGYDVVSAPPPVGG
                     VTVIEILQMLGTFELSGETPLSPRYVHLAAEAMKRGFADFTAYVGDPDFVTVPVADLL
                     SRDYARRRASEIRSDAVTPKVAAGDPGKREPPSTTSLAAVDARGNMVALTQTISDFFG
                     AKVMAGATGIILNNEMKNFGARGPNAIAPGKRMRTMIAPTILLRGGKTFAAIGTPGAA
                     RIVSTMTLLVSNLVDFGMGIQEAIEAPRFYARDVEADLSVEARVPAATIEALTKMGYS
                     VKTLGEYDLFFGGAQGIVIDPRTGLRIGGADPRRDGAVVGY"
     gene            complement(4516..4938)
                     /locus_tag="PLT35_04005"
     CDS             complement(4516..4938)
                     /locus_tag="PLT35_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28420.1"
                     /translation="MARTSFRKLTPAAAVGRVAVALAAARELDASVRIPPAFTAADSS
                     LVRALPPWVSRTALLRSARAWWLWCEPRKGRARDGQCVIDVPAGRYLVETLDTRSHTW
                     VSCESAAGGPLVMGLPFTGRPVLACIRRIAPAAAVPRE"
     gene            complement(4940..6022)
                     /locus_tag="PLT35_04010"
     CDS             complement(4940..6022)
                     /locus_tag="PLT35_04010"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOG28421.1"
                     /translation="MSLRARAAATVLAAGLLAASAALSKPAAQVAVPPGAPAVHDIRP
                     GPGVTAVKMLSEYAPGLAGTPGDTRVYVLDGKEPGATVFVAGGTHGNEIAGIVAAVVL
                     VERARVARGRLIVVPHANNSAITDVDPERPGPPSIAVATLGGERRFLYGSRRTKLAHQ
                     GAPDPAIYRHPNPKADEDLPGAEARNLNRAYPGSADGTLTQRMAYAVMQVLERERAAV
                     AFDFHEAGPESRLAWMIVANPKNLETAAVAILDLEAQGLSMKLEPSSETFRGLSHREW
                     GDATAAQAYLFETPSPSMADDTKGVDFVNDPRLPLARRVGGQLASFSAVLAAYNADAA
                     PDARAICLDVPTMAEVEKAGVGAFLR"
     gene            6131..8422
                     /locus_tag="PLT35_04015"
     CDS             6131..8422
                     /locus_tag="PLT35_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOG28422.1"
                     /translation="MSDRPHIRRLPLGRGAARPAARGGTSTVHGRPGRKAPPCPGGEA
                     GTLSGTVERVTFHNPDTGFCVLRVAVPGRRDAETVVGHVASIAAGEEIRAAGEWTNDI
                     VHGLQFRATSIHAAPPSSLEGIERYLGSGFIRGIGPVFARKLVQAFGPGVFDVIDAQP
                     SRLRDVDGIGPARAAQIVEAWSGQKVIRDIMVFLYGHGVGTSRAVRIYKAYGADAIAL
                     VKENPYRLARDIRGIGFLTADALARRLGIEKTAGIRARAGIAYALMQAIDEGQCGLPR
                     EDLLASAQKLLEVPAETVGAALQAELDEGAVVADAAAGRDCVFLAWLHSTERQLADAL
                     RGLAAGTPPWAAIDAGKALEWVERNLDLTLADRQREAVRQAIASKALVITGGPGVGKT
                     TIVRAILAILSAKRVRVALCAPTGRAAKRLTEVTGLEAKTIHRLLEVNPADGRFRRGP
                     SSPLACDVLVVDETSMVDVPLMHALVRAVPPRAAVIFVGDVDQLPSVGPGQVLEDIIS
                     SGAVPVVRLTEVFRQAAASRIVVNAHRVNRGEMPELTTTPDRPTDFYFVEIDTPEEGL
                     PRIIQIVTERIPQRFGLDAVRDVQVLSPMNRGAIGARSLNAELQKALNPRPPQAVQRF
                     GTTFALGDKVMQIENDYDKDVYNGDIGFVRAIDAELGDVTIEFDGRPVLYASHELDRV
                     VLAYATTVHKAQGCEYPAVVIPVTTQHYPMLQRNLIYTGITRGRQLVVLVGQKKALAM
                     AVRGSRAVRRWSKLGEWLAGGPR"
BASE COUNT         1170 a         3086 c         3023 g         1153 t
ORIGIN      
        1 ccccatcggg ctggcggccg tcacgctggc gctcgtctcg gtggccgtcg tcaacctgtt
       61 caccaagcag gtcgcgacca tcgccggggc cgggttcacc gtcacgctct atgcggcgtt
      121 cacggcctcg gagcgggcca cggcgaggcg gcaggacgcc gcgcgcaagc tggatcagtt
      181 ccacctgctt cccgtgccgg acatcgggct ggacgtgctc gcggcgcggc ccggcggcgt
      241 gctcgtgccc gtccgggacc cccgcacgct ggcgcacctg aagtgggcgc tggcccgcac
      301 cgacacgcag aagcaggacc tcgtcgtcat gacggtgcgc ccgctgcagg ggccggacac
      361 cggctaccgg gacttcgcag gcgagcgcgt cttcagggag tacgagcagg agctcttcac
      421 gcgcgtcgtg gcggtcgccg agcgcgaggg acggcccgtc aagctgctgg tcgtgccctc
      481 gagcaacgtc gcggatgcca tcgcgcggac cgccgtcagc ctgcaggcct ccgacaccgt
      541 catcggggag tcggccaccc tgagcggtgc ggaaatggcc cagcggatcg gggaggcctg
      601 ggaccgcgtt ccgaagagcc aacccgtgcg ctcgaggctc gtggtattca ggctggacgg
      661 gccgcccgag atgttccacc tcggacccca tccgcccgcg ctcagccagg aagacctcga
      721 cctcatacac aggatctggc aggacgccgt caggcagttc gggctcggcg tgcaccaccg
      781 cgacgtcgtg gccgtcgcgc tggaacaact cgaacgggac cttgccggag acggccgcga
      841 gcgcgcgctc gcgcggatcg ccgagcggtc cgggcgaccg cccgcgccgc ccgcggagag
      901 cgatgaattg cgcccgggca cgtagaggcg ggcgccgatg gcgcccgcgc cgagcagcac
      961 ctccggctcg cgcgaaggcc gcggagcggc gccgactgga tcaggcggcg ggagcgtcgc
     1021 ggcagatggg gtcagatcca aaaagacggc cccgcgcatc gaaacggggc cgtctttttg
     1081 gatctgaccc cagagcgccc caaggagcga gcagatgaac atggcaacgc gctggcgggc
     1141 tgccgggatc atcaccgcgt cggtcttgat gttcggactg ggcgcatcct cgcagaccca
     1201 ggagccgccg ctcgtgcggt ttggcgtgat caccgacctc cactacgccg acatcgaccc
     1261 ggccggcccg agggtctacc gcgactcggt ggcgaagctc gaggcgtgcg tgagaacgat
     1321 gaacgccgag cgggtcgcgt tcctggtgga gctcggcgac ttcaaggacc aggacacgcc
     1381 gccggtggaa tcgcgcacgc tctcgtacct gaggaccatc gaggcggcga tggccggctt
     1441 caagggaccg cggtatcacg tcctcggcaa ccacgacgtt gacagcctct cgaaggcgca
     1501 gttcctcggc gtggtcgaga acaccggcgt cgcgccgccc gcgacgcggt acaccttcgt
     1561 gtccggcggg gtccgcttcg tggtgctgga cgcctgccac aaggcggacg gttcggacta
     1621 cgaccgcggg aactacgact ggaccgacag caacatcgat gcgccgcaac tggcctggct
     1681 gtcgtcaacg ctcgccggct cggccgagcc ggtcatcgtg ttcgtgcacc agcagctcga
     1741 cgggacgggt tcgtactacg tgaagaacgc gccggacgtc aggcgggtgc tcgagcgatc
     1801 ggggaaggtg ctcgccgtgt tccagggcca ccgccacgcg ggcgccttca gcgagatcga
     1861 cggcattccg tacttcaccc tcgcggcggc cgtcgagcag cccggcgcgg agaacggcgc
     1921 gtactcgatt gtcgaagtgg ggcgcggcgg cggcatccgc gtcatcgggc gccagcgcgc
     1981 gacgtcgcgc acgacgacgc ggtgacggcc tccgatcccg ggcgcggatc gcggcccgga
     2041 accggagacg gccgcctcgc cgggcgtcca gctactgttt gaacagggcg cgcaccgcgc
     2101 cgccggcgtc cgcgagcttc gccacttccg tcaccggcac gccgtgcttc tcgccgaggg
     2161 tcgtgaacag cttgtccttg ttgccggccg ccacgacgac gacgtggtcc gccagcggca
     2221 gcaccgcctg gatgaggtcg ttcgacgtct tgccccgccg cgcttcgccg cccgtgtgca
     2281 gcaccagcac gcggaggcct tccgacttcg ccgccttgag cagcgccgtc gtgcgcgcga
     2341 tttccctggg catgtcgagg ccggccgccc cgaggccctt cgtgctcgcg ccgaccgaga
     2401 cgacgagcgc cttcatgccg gcgagatccg acggctgcgc aatgggcttg tactcgaggc
     2461 ccagcttgag ctgcgtgttc agcatcacct tgatgatggc cacgtcggag ctctgtccgg
     2521 ccgacgtgat gagtcccggc gtctcgaaga ccgcctgcgc gtccgccgcc gggaccgcgc
     2581 acgccaacac cgcgaatgcc accgccgcca gccgtacgac gtgtctgcct gccatcgatt
     2641 cccccgctcg tccctcgaat cgagtcacca gccccaagcg cccagcgcct ggcctccagc
     2701 ccgagccccc gccttcgcta gccggcctgc cggcctgccc tgagcttgtc gaggggccag
     2761 cttcggcgcg gcgagcttcg tcccttgccc ctgtcttcct ggcgcccagc cccggcccct
     2821 ggcccctagt atcccaccac cgccccgtcc ctgcgcggat cggcgccgcc gatgcgcaga
     2881 ccggtcctcg gatcgatcac gatgccctgc gctccgccga agaacaagtc gtactcgccg
     2941 agcgtcttga cgctgtagcc catcttcgtc agcgcctcga tcgtcgctgc cggcacccgc
     3001 gcctcgacgg acaggtcggc ctcgacgtcc cgcgcgtaga accgcggggc ctcgatggcc
     3061 tcctggattc ccatgccgaa gtcgaccagg ttcgacacga gcagcgtcat ggtcgagacg
     3121 atgcgcgccg cgccgggcgt gccgatggcg gcgaaggtct ttccgccgcg caggaggatc
     3181 gtcggcgcga tcatcgtgcg catccgcttg ccgggcgcga tcgcgttggg cccgcgcgcg
     3241 ccgaagttct tcatctcgtt gttcaggatg atgccggtgg cgccggccat gaccttcgcg
     3301 ccgaagaagt ccgagatcgt ctgcgtcagc gccaccatgt tcccgcgcgc gtccacggcg
     3361 gcgagcgagg tcgtgctggg cggctcgcgc ttcccggggt cgccggccgc gaccttcggc
     3421 gtgacggcgt cgctcctgat ctcggaggcg cggcgccgcg cgtagtcccg cgacagcaga
     3481 tcggcgaccg gcaccgtgac gaagtccggg tcgccgacgt aggccgtgaa gtcagcgaag
     3541 ccgcgcttca tggcctcggc cgccaggtgc acgtagcgcg gcgagagcgg cgtctccccg
     3601 gacaactcga acgtgccgag catctgcagg atctcgatga cggtgacgcc gcccaccggc
     3661 gggggggccg acacgacgtc gtagccgcgg tacgtcccct gcaccggcgc gcgctcgacc
     3721 gcccggtacg ccgcgaggtc ggccctggtg atgcggccgc cgctcgccgc catgtcggct
     3781 gcgatcgcgt ccgccagcgc gccgcggtag aacgccgccg cgccgccagc cgcgatcgtc
     3841 cggaggctcg cgccgagcgc cgggttcttc agcgtggcgc ccgattcgat cggcaggccg
     3901 ccggggcaca ggattgccgc cagcgcctcg ttcttcgcga tggcctcgaa gttgtcggcc
     3961 acgatgcccg caagcgtggg gctgacgacg aagccccgct cggccagacg gatggccggc
     4021 gccacgacgc gcgcgagcgt catcgacccg tacttctcga gggccattgc caggccggcc
     4081 acggtcccgg gcacggcgac cgccgcgtga ccggcatcgg gaatcggccc ggggaagtcg
     4141 atggccgccg gggcggacga ccggtagtcg acagcgaccg cctgcttcga gtcggcgagg
     4201 tagatgacca tcatcccctc gccgccgagg ccgcttgcgt tgggctcgac cacgcctatc
     4261 gcgaaggccg ccgccacggc ggcgtcaacc gcgttcccgc ccgccttcag gatctcgagg
     4321 ccggcctggg acgcgagcat gtgcgccgac gccaccatgc cgcgtttcgc ggtgaccggg
     4381 gcgctgcgcg tgacggcgcc tgtgcgcggg agcgccggcg cggcgacgat gagggcggcg
     4441 gccagcgttc cggtgaggaa ggcttgtcgg atcatggcgg cctcgatggc gacggcgccc
     4501 cggcggcgct cgcgttcact cgcgcggcac cgccgcggca ggcgcgatcc ggcggatgca
     4561 ggcgagcacc gggcgcccgg tgaacggcag gcccatcacg agcgggccgc cggcggcgga
     4621 ctcgcacgac acccacgtgt ggctccgcgt gtcgagcgtc tcgacgaggt accggcccgc
     4681 cggcacatcg atcacgcact ggccgtcccg cgcgcgcccc ttgcgcggct cgcaccacag
     4741 ccaccaggcc ctggcggacc tcagcagggc cgtcctcgac acccacggcg gcagcgcgcg
     4801 caccagcgac gaatcggccg cggtgaacgc cggcgggatc cggaccgacg cgtccagctc
     4861 ccgcgcggcg gccagcgcga cggccacccg gccgaccgcc gcggccggcg tcagcttcct
     4921 gaacgacgtg cgtgccatgt cacctcagga acgcgcccac gcccgctttc tccacctcgg
     4981 ccatcgtcgg gacgtcgagg cagatcgcgc gcgcgtccgg cgcggcatcc gcgttgtagg
     5041 ccgcgaggac ggccgagaac gacgcgagct gcccgccgac gcgcctggcc agcggcagcc
     5101 tcgggtcgtt cacgaagtcc acgcccttcg tgtcgtccgc catcgacggg ctcggcgtct
     5161 cgaacaggta cgcctgcgcg gccgtggcgt cgccccactc gcggtgcgag agcccgcgga
     5221 aggtctccga cgagggctcg agcttcatcg acaggccctg cgcctcgagg tcgaggatcg
     5281 cgacggccgc cgtctcgagg ttcttcgggt tcgccacgat catccacgcc aggcgcgact
     5341 ccgggcccgc ctcgtggaag tcgaaggcga cggcggcgcg ctcgcgctcc agcacctgca
     5401 tgacggcgta cgccatccgc tgcgtcagcg tgccgtcggc cgagccgggg tacgcgcggt
     5461 tcaggttgcg cgcctcggcc cccggcaggt cctcgtctgc cttcgggttc ggatgacggt
     5521 agatcgccgg gtcgggcgcc ccctggtgcg ccagcttcgt ccggcgcgag ccgtacagga
     5581 agcgccgctc gcctcccagc gtggccacgg cgatcgacgg cgggcccggc cgctcgggat
     5641 cgacgtcggt gatggcggag ttgttggcgt gcggcacgac gatgagccgc cccctggcca
     5701 cgcgggcgcg ctcgacgagc accacggcgg cgacgatgcc ggcgatctcg ttgccgtgcg
     5761 tgccgccggc cacgaacacg gtggcgccgg gctccttgcc gtcgagcacg tacacgcggg
     5821 tgtcgcccgg cgtgccggcc agtccgggcg cgtactccga cagcatcttc acggccgtca
     5881 cgccggggcc gggccggatg tcgtgcacgg cgggcgcgcc gggagggacc gccacctggg
     5941 cggctggctt cgagagggcg gccgacgcgg ccagaaggcc ggcggcgagg acggtggccg
     6001 ctgctcgtgc gcggagtgac acggtcgtct ccagaggggc aggctgctgg ctcgccgccc
     6061 cgatcaggcc gccattctgc cacggaatgc ccgacggtga gagaatgagg cgctccgggg
     6121 gccatccatc atgagcgacc gcccgcacat tcgccgcctg ccgctcggac gcggcgccgc
     6181 ccggccggcg gcacgaggcg gcacgtccac agtccatggg cgcccggggc ggaaggcgcc
     6241 gccgtgcccg ggaggggagg cggggacgct gtcgggcacc gtcgagcgcg tgacgttcca
     6301 caaccccgac accggcttct gcgtgcttcg cgtcgccgtg cctggccggc gcgacgccga
     6361 gacggtggtg ggccacgtcg cgtccatcgc ggcgggcgag gagattcgcg ccgccggcga
     6421 gtggacgaac gacatcgtcc acgggctgca gttccgcgcc acgtccatcc acgccgcgcc
     6481 gccgagcagc ctcgagggca tcgagcggta cctgggctcc ggcttcatcc gcggcatcgg
     6541 gcccgtcttc gcgcgcaagc tcgtccaggc cttcggcccc ggcgtcttcg acgtgatcga
     6601 cgcgcagccc tcgcggctcc gcgacgtgga cggcatcggc ccggcgcgcg cggcgcagat
     6661 cgtggaggca tggtccggcc agaaggtcat ccgcgacatc atggtcttcc tgtacgggca
     6721 cggcgtcggc acgtcgcgcg ccgtgcgcat ctacaaggcc tacggcgccg acgcgatcgc
     6781 cctcgtcaag gagaacccct accgcctggc gcgggacatt cggggaatcg ggttcctcac
     6841 cgccgacgcg ctcgcccgcc ggctcggcat cgagaagacc gcggggatca gggcccgggc
     6901 cggcatcgcg tacgcgctga tgcaggccat cgacgaagga cagtgcggcc tgccgcgcga
     6961 ggacctgctg gcgtcggcgc agaagctgct cgaggtgccg gccgagaccg tcggcgccgc
     7021 gctccaggcg gagctcgacg agggcgccgt cgtggccgac gccgcggccg ggcgcgactg
     7081 cgtattcctg gcctggctcc actcgacgga gcgccagctc gccgacgcgc tgcgggggct
     7141 cgcggccggc acgccgccgt gggcggccat cgacgcgggc aaggcgctcg agtgggtcga
     7201 gaggaacctc gatctgacgc tggccgatcg tcagcgcgag gccgtgcggc aggccatcgc
     7261 gtcgaaggcg ctcgtcatca ccggaggtcc gggcgtcggc aaaaccacga tcgtgcgagc
     7321 cattctcgcg atcctctcgg cgaagcgcgt gcgcgtcgcg ctgtgcgcgc cgaccggccg
     7381 cgcggccaag cggctcaccg aggtcaccgg cctcgaggcg aagacgatcc accggctcct
     7441 ggaggtgaac ccggccgacg ggcgcttccg gcgcgggccg tcgagccccc tcgcctgcga
     7501 cgtgctggtc gtggacgaga cgtcgatggt ggacgtgccg ctcatgcacg cgctcgtgcg
     7561 cgccgttccg ccgcgcgccg ccgtgatctt cgtcggcgac gtcgatcagc tcccgtccgt
     7621 cggcccgggc caggtgctcg aggacatcat ctcctccggc gctgtcccgg tggtccgcct
     7681 gacggaggtc ttccgccagg ccgccgccag ccgcatcgtc gtcaacgcgc accgggtgaa
     7741 ccgcggcgag atgcccgagc tgaccacgac gccggaccgg ccgacggact tctacttcgt
     7801 cgagatcgac acgcccgagg aggggctgcc gcgcatcatc cagatcgtga ccgagcgcat
     7861 cccccagcgg ttcggcctcg atgccgtgcg cgacgtgcag gtgctcagcc cgatgaaccg
     7921 cggcgccatc ggcgcgcggt cgctgaacgc cgagctccag aaggcgctga acccgcggcc
     7981 gccgcaggcc gtccagcgct tcggcaccac cttcgcgctg ggcgacaagg tgatgcagat
     8041 cgagaacgac tacgacaagg acgtctacaa cggcgacatc gggttcgtgc gcgcgatcga
     8101 cgcggagctg ggcgacgtga cgatcgagtt cgacgggcgg cccgtcctgt acgcgtcgca
     8161 cgagctggac cgggtcgtgc tcgcctacgc gacaaccgtg cacaaggcgc agggctgcga
     8221 gtatccggcg gtcgtgatcc ccgtgacgac gcagcactac ccgatgctgc agcggaacct
     8281 gatctacacg gggattacgc gcgggcggca gctggtggtg ctggtggggc agaagaaggc
     8341 gctggcgatg gccgtgcgcg gatcgcgcgc cgtccgccgc tggtcgaaac tgggggagtg
     8401 gctggcggga ggcccgcgat gagccgccgg cc
//
LOCUS       DAOSIF010000073         8414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_7889_length_8414_cov_3.530566, whole genome shotgun sequence.
ACCESSION   DAOSIF010000073 DAOSIF010000000
VERSION     DAOSIF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_8414_cov_3.530566"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..8382)
                     /locus_tag="PLT35_04020"
     CDS             complement(<1..8382)
                     /locus_tag="PLT35_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG28423.1"
                     /translation="MAQTATGESPPHQRVTQAGADGKLGTGDDITTLGPGETGNAEYL
                     VEGRREGTWVVEMELTGTLNGMPVGPVPVRGRAAGVVLVRNPKFTLTFTHPDVVNAGE
                     PYTLDVTVTNTSGAPANFVSVNLYSHNISGAALDDEPTKSVEYIAPDDSATMTFKLIS
                     QRTGIVTAATLDSAEQVEGRFALKTAVGELGVPLSPDSLVLPKEAGSLPKAVRDAALG
                     LLGRAWAVATAPPIVFPKDLERFSKQIVLGRAVETAEAGFRILLGAPVPQAVADLWMD
                     MLGSEYSRLGERVEDTDPPEMLSLLQADVRGFDLLRRKSVRGDALADQVASAVLPWLG
                     AAGAAPFHQAIAAQFTSRPGHLSILVAGDGASDVPVDAVLTDAAGRRLGGLDAGRTVK
                     EIPYGDVLTFRNGDTVTARLLLVAVPEPGAFHVTLSRKAGAAPGALYGVSVAYPGADL
                     RLRFASFPDLGPAEVPALQQPAGDPYLVTFSLAGAASPQAREAAGAAVTDPPPSVLGA
                     VQMAEADVVGCSAIGLENRRVHAGRVIAVLFSEEVTPESVQDAVTDQGITAFAVEGNR
                     VLDVALQPGNRIAYVALREPVGRFVDRTITVTGVADTRGNVLASASVPIQATVTDAGG
                     VVSGKVLNADGTPAQFAEVRLFYEFRCDEEGTVVVGIASDFADAEGRYQFDYVSLPVA
                     AVKMVAINTEADDLRTQRFTVSRDGQLLNLNMVFLGRGAFAGRTLAEDGVTPLANTSL
                     RVTSLTDESQYGATSDAAGAFEIRRIPVGNILVEAVNTARPASVFLSDYIPSAGATVT
                     RDLVLLDVSTTQITVKTGSVSGRVLRSDGTTRVPGVQVVAYYRNESQPGVRCSPPPGS
                     LSGAVPSECAIAIATTLDDGSFVFPAISAGELRLYTFDQAALQEGGVRFLLAEDQALD
                     VSLLLGGGLGTVHGVVLDGAGNPVPDAEVGGGLSLTTVNPADGTFTLTDVPVGRRALV
                     AVSASLGATGRTTIDIVREGEVVNAAIVLESMGAVAGIVRDRGGAPQPGIHVYVIQPC
                     YDDLGREITCVKGEATTDANGAYRVNGLVTGSYTVSAFLPDMSDGNLAQAAIRYHGQV
                     LKTDITFRGGSGTVTGRVLRAKPAGCDEADPSCAETPLPARVAISGDRLVTAGGIVGV
                     AFQYVQHFAIVDNNMTTGAFTFTNVWTGPFTVRAAGQFSPEPVAAEGVMPGPGETVTV
                     DLRLQPTSVITGVVLEPDGVTPVTGRQVSLRFSSNAVVVFCSEESGTGMTSCTSIPQG
                     IQEEYAVTDDTGRFSFPLVNPGAFTVTATDPGTGTGGHVAEVKGAVKPGETLDLGIRL
                     LGAARATVNVYSHDGQTPIPNAAVELQQIDYPKASRSGTAVSGTITFDGLGEGQFVVK
                     ALGPGGFAGRASGAITGEGADAVVNVYLYDATGTVTGLVTRLDSEGTAVAVPNAEVIL
                     SSGGKPIAFAVTDSGGSYTVTLVPVGDFRIEVFDPVTAGRGRASGTVTGGAIPIEANV
                     LLDALGVVRGVVVESGTLDPLKGWQVRLAQVTPSGRSLPALVTMTSVDGSYSFPGASV
                     GTFSVRAWKSGVVGEAAVTGEVSRPGQLVEVPIVVTVVRRVTGTVAGTVFNPDGSAAS
                     NAQIDVCPASEACRPTVNSPDGRFSIGDIPLGRFTVKARAQVTGNPSAGTAGGTLLFE
                     GDTADVQVTLVGLGAVQGRVFEIVNSVKTPAAYASVLLYGQPGSGCAGPCHQSTDANG
                     AFSFVNVPARTFTVAASKEGQRGSVGDTLNAGETKTGLEVILAPAVSFTGRVRLANGL
                     PAIGIVADLARGTSHLYAESDPDGVFSYDAVGSGPYVLLLQDPVGGGIARLSGTILAN
                     TPVDLGDILLDEQPPAVAATSPAAGEFGVSLTPEIRVTFTEAVSPGTVTAANVSVSGP
                     SGPLTGLLDLVDGDTVARFRLLSGSVLDDHARYTIRVRGVEDRYGKPMAGETVVSFTT
                     ADSTPPSVIETTPVASASGVPIDSVVRVKYSEIIDPSRFAGPAITVSAPGGAVAGAVE
                     FILGNTVVVFTPALPLARDTTYAVHVAAATDLSGQTQASGVDFTFTTTDSTPPQVTAL
                     VAANGGRVIENTVAVVTATVSAGDVAFVDFLVNGALTSTVRTARDGSGAILPFTLAFQ
                     ATPGFGAPGSLIGVAAVATDTSGNRGPASSTVVEVVADAPPAAEITAPPAGAHAAGGA
                     RVTVRVHAADDVGLAQAGYVARTTAGIIQAATSAFTGAPLDATAEFAFTVPAGLAPGA
                     VITVEASAVDVKAQATAAAPVTLSVTDTAGPAVAITGVTSGERVRPGQRVTIVVTAED
                     AGLVARVGFAAIGAGVATSGDRDVSPAQASVATSFSFDVPVTADSGEQVDLSAYAIDA
                     AGNRTEAAHKILTVADAVAPAATLRTSDGSLFIVPGSTVSIVVTGSDDMAVATLALTG
                     SGAFTFSDSFSVTAPVSTASHTFVVSVPAGVAHGATVLATAIVTDVSGNVSTPVSLTL
                     TAKSVVDVTLPGSVVLRAGERADLAVGLTAPAASAVVVILQSSAPGVATVTSSVAFSA
                     GETTKAAAITGVAGGTAQITAYVSGVARATSTATVIGGVVRGTVGDPDPVAGAQVTIF
                     HGGAPIAAVTDASGAFDVEGVIGTGTGGRAFTVRAAAGELLGYADALLSVRGGHASVS
                     VMLVPLSTIAGTVYHADGATAAGEGVQVDLYEAASPSTIVSTAFTNAEGRFEFRLVAQ
                     GTYILVGSDLAGNKGRTTVSVGASGQEVTADIGFLGRGTVTGIVRDGAGAAVAYAQVE
                     LHSSSIFGTVTRTATAGAEGTFAF"
BASE COUNT         1170 a         3099 c         2987 g         1158 t
ORIGIN      
        1 gaaggcgaag gtcccctcgg cgcccgcggt cgccgtacgg gtcacggtcc cgaagatgct
       61 cgacgagtgc agctcgacct gggcgtaggc gaccgccgcc cccgcgccgt cgcgcacgat
      121 accggtcacg gtgccgcggc cgaggaagcc gatgtccgcc gtcacttcct ggccgctcgc
      181 gccgaccgaa accgtcgtgc gccccttgtt gccggccagg tcggacccga ccaggatgta
      241 ggtcccctgg gccaccaggc ggaactcgaa ccggccctcg gcgttcgtga acgccgtgct
      301 gacgatcgtg gaggggctgg ctgcctcgta cagatcgacc tgcacgccct ctccggccgc
      361 cgtcgcgcca tcggcgtgat acacggtgcc ggcgatggtc gagagcggga cgagcatcac
      421 ggagacggac gcatgtccgc cgcgcaccga cagcagcgcg tcggcgtatc ccagcagctc
      481 gcccgccgcc gcgcgcaccg tgaaggcccg gcctcctgtc ccggtcccga tgacgccctc
      541 gacgtcgaac gctccgctcg catcggtcac ggccgcgatc ggcgcgccgc cgtggaagat
      601 cgtcacctgc gctcccgcca ccgggtccgg gtcgccgacc gttccgcgca ccacgccgcc
      661 gatgaccgtc gcggtcgagg tcgcccgcgc cacgcccgac acgtacgcgg tgatctgcgc
      721 cgtgccgccc gcgacgcccg tgatcgcggc ggccttcgtc gtctctccgg cgctgaaggc
      781 cacgctggac gtgaccgtcg ccacgcccgg cgcgctcgac tggaggatga ccacgacggc
      841 cgacgcggcc ggcgccgtca gcccgaccgc gaggtccgcg cgctctcccg cgcgcagcac
      901 cacggacccg ggcagcgtca cgtccacgac cgacttcgcc gtcagcgtca ggctcaccgg
      961 cgtgctgacg ttgcctgaca cgtccgtcac gatcgcggtc gcgagcacgg tggcgccgtg
     1021 ggcgacgccc gccgggacgc tgacgacgaa cgtgtgcgac gccgtggaca cgggcgcggt
     1081 cacggagaag ctgtccgaga acgtgaacgc gccgctcccg gtgagcgcca gcgtcgccac
     1141 cgccatgtcg tcgctgccgg tgacgacgat gctgacggtg gaacccggca cgatgaagag
     1201 actgccgtcc gaggtccgca gcgtggcggc gggcgcgacc gcgtccgcca ccgtcaggat
     1261 cttgtgcgcc gcctcggtcc ggtttccggc cgcgtcgatc gcgtaggccg agaggtcgac
     1321 ctgctcgccc gagtccgccg tcaccggcac gtcgaagctg aaggacgtgg ccacgctcgc
     1381 ctgcgccggg gagacgtcgc gatcgccgga cgtggccacg ccggccccga tggccgcgaa
     1441 cccgacgcgg gccacgaggc cggcgtcctc cgccgtcacg acgatggtca cgcgctgccc
     1501 cggcctgacg cgctcgccgg acgtcacgcc ggtgatggcg acggcgggcc cggcggtatc
     1561 ggtcaccgac agcgtgacgg gcgccgccgc cgtcgcctgg gccttcacgt ccaccgccga
     1621 cgcctcgacg gtgatcacgg cgcccggcgc gagcccggcg ggcacggtga aggcgaactc
     1681 cgcggtggcg tcgaggggag cgccggtgaa cgccgacgtc gcggcctgaa tgatccccgc
     1741 cgttgttcgg gcgacgtagc cggcctgcgc cagccccaca tcgtcggcgg cgtgcaccct
     1801 cacggtcacg cgggcgccgc cggcagcgtg cgcgccggct ggcggcgccg tgatctcggc
     1861 tgcgggaggc gcgtcggcta ccacctcgac gaccgtcgag gacgcgggac cgcggttgcc
     1921 cgacgtgtcg gtggcgaccg ctgccacgcc aatcaggctg ccgggcgcgc cgaagcccgg
     1981 cgtggcctgg aaggccagcg tgaacggcag gatcgccccc gatccgtcgc gggccgtgcg
     2041 aaccgtcgaa gtcagcgcgc cgttgacgag gaagtccacg aacgcgacgt ccccggccga
     2101 gaccgtcgcc gtcacgacgg ccaccgtgtt ctcgatcacg cgtccgccgt tggcggccac
     2161 cagggccgtc acctggggcg gcgtgctgtc ggtcgtcgtg aacgtgaagt ccacgcctga
     2221 tgcctgggtc tggccggaga ggtcggtcgc cgcggcgacg tggacggcgt atgtcgtgtc
     2281 ccgcgccagc ggcagcgccg gcgtgaacac cacgacggta ttgccgagga tgaactcgac
     2341 agcgcccgcg acggcgccgc ccggcgcgct cacggtgatg gccggaccgg cgaacctgga
     2401 ggggtcgatg atctccgagt acttgacccg cacgacgctg tcgatgggca cgccgctcgc
     2461 cgacgcgacg ggcgtggtct cgatcacgga cggcggcgtc gagtcggccg tggtgaacga
     2521 cacgaccgtc tcgccggcca tcggcttgcc gtagcgatct tcgacgccgc ggacgcggat
     2581 cgtgtaacgc gcgtgatcgt cgagcacgga cccggacagc aggcggaagc gcgccacggt
     2641 gtcgccgtca acgaggtcga ggaggcccgt caggggcccg gacggcccgc tcaccgacac
     2701 gttcgcggcg gtcaccgtgc cggggctgac ggcctcggtg aaggtcaccc ggatctccgg
     2761 cgtcaggctc acgccgaact cgcctgccgc cgggctcgtg gcggccaccg caggcggctg
     2821 ctcgtcgagg aggatgtcgc cgagatcgac gggcgtattc gccaggatcg tcccggacag
     2881 acgcgcgata ccgccgccga cggggtcctg cagcagcagg acgtaggggc ccgagccgac
     2941 ggcgtcgtaa ctgaaaacgc cgtccgggtc cgactcggcg tacaggtgcg acgtgccgcg
     3001 ggccaggtcc gcgacgatcc cgatggccgg caggccgttc gccaggcgca cgcggccggt
     3061 gaagctgacg gccggcgcca ggatgacctc gaggccggtc ttcgtctccc cggcgttgag
     3121 cgtgtcgcct acggacccgc gctggccctc cttcgaggcg gccacggtga acgtgcgcgc
     3181 gggcacgttg acaaagctga aggcgccgtt ggcatccgtg ctctggtggc atggcccggc
     3241 gcacccgctg cccggctgcc cgtagagcag gaccgacgcg taggccgccg gtgtcttgac
     3301 gctgttgacg atctcgaaaa cgcgcccctg aacggcgccg agcccgacca gggtgacctg
     3361 cacgtccgcc gtgtcgccct cgaagagcag cgtgccgccg gccgtgcccg cgctcgggtt
     3421 gccggtgacc tgcgcgcggg ccttgacggt gaaccggccg agcgggatgt cgccgatcga
     3481 aaaccgtccg tccggcgagt tgaccgtcgg gcggcacgcc tcgctggcgg ggcacacatc
     3541 gatctgcgcg ttcgacgccg cgctcccgtc gggattgaag acggtccccg ccacggtccc
     3601 ggtgacgcgg cgcacgacgg tgacgacgat cggcacctcc acgagctggc ctggccgcga
     3661 gacctcgccc gtcaccgcgg cctcgcccac cacgccgctc ttccaggcgc gcacggagaa
     3721 cgtgccgacg gacgcgcccg ggaacgagta gctcccgtcc acgctggtca tcgtcaccag
     3781 cgccggcagg ctccggccgg agggcgtgac ctgggccagc ctgacctgcc atcccttcag
     3841 cgggtccagc gtgccgctct cgacgacgac gccgcggacg acgccgagcg catcgagcag
     3901 gacgttggct tcgatgggga tcgccccgcc ggtgaccgtg ccgctcgcgc gcccgcgtcc
     3961 cgccgtcacc gggtcgaaca cctcgatgcg gaagtccccc accggcacga gcgtgacggt
     4021 gtagctgccg ccgctgtcgg tgacggcgaa cgcgatgggc ttccctccgg acgacaggat
     4081 gacctcggca ttcggcacgg cgacggcagt cccttcgctg tcaagacgcg tgacgaggcc
     4141 cgtcaccgtg cccgtggcgt cgtagaggta cacattgacg acggcgtcgg cgccttcgcc
     4201 ggtgatcgcc cccgacgcac gcccggcgaa tcctcccggg ccgagcgcct tcacgacgaa
     4261 ctgcccctcg ccgagcccgt cgaaggtgat ggtcccgctg acggccgtgc cgctcctcga
     4321 cgccttcggg tagtcgatct gctggagttc gacggcggca ttcgggattg gcgtctgccc
     4381 gtcgtgcgag tacacgttga ccgtcgcccg tgcggcgccg agcaggcgga tgcccaggtc
     4441 gagggtttcg cccggcttga cggcgccctt cacctcggcc acgtggccgc cggtcccggt
     4501 cccaggatcc gtcgccgtca ccgtgaacgc cccggggttg accagcggga acgagaagcg
     4561 gcccgtgtcg tcggtgaccg cgtattcctc ctggatgccc tgcgggatgg acgtgcagga
     4621 cgtcattccc gtgcccgact cctccgaaca gaacacgacg accgcgttcg acgagaacct
     4681 gagcgacacc tggcggccgg tgaccggcgt cacgccgtca ggctcgagca cgacgcccgt
     4741 aatgacgctc gtcggctgca gccggagatc gacggtgacc gtctcgccgg gccccggcat
     4801 gaccccttcg gcggccaccg gctcgggact gaactggccg gcggcccgga cggtgaacgg
     4861 gccggtccag acgttcgtga acgtgaacgc gcccgtcgtc atgttgttgt cgacgatggc
     4921 gaagtgctgg acgtactgga acgcgacgcc gacgatgccg ccggccgtca cgaggcggtc
     4981 gccggagatc gcgaccctgg ccggcagcgg cgtctcggcg cacgacgggt ctgcttcgtc
     5041 gcagccggcg ggcttcgcgc gcagcacgcg tccggtcacc gtcccgctgc cgccgcggaa
     5101 cgtgatgtcg gtcttcagga cctggccgtg gtaccggatc gcggcttgcg cgaggttccc
     5161 gtcgctcatg tcgggcagga acgccgacac ggtgtaggac ccggtcacga ggccgttcac
     5221 gcggtaggcg ccgttcgcgt cggtcgtcgc ctcgcccttg acgcaggtga tctcgcggcc
     5281 caggtcgtcg tagcagggtt ggatgacgta gacgtggatg cctggctgcg gggcgccgcc
     5341 acggtcgcgc acgattcccg ccacggcgcc catcgactcg agcacgatgg ccgcgttcac
     5401 gacctcgccc tcgcggacga tgtcaatcgt cgtcctgccc gtcgcgccga gcgacgcgct
     5461 caccgccacg agcgcgcggc gacccaccgg cacgtcggtc agcgtgaacg tgccgtcggc
     5521 cgggttgacc gtcgtcaagc tgagcccgcc gccgacttcg gcgtccggca cgggattgcc
     5581 cgccccgtcg agcacgacgc catgcacggt ccccagcccg ccgccgagca gcaggctcac
     5641 gtcgagcgcc tggtcttccg cgagcaggaa cctgacgccg ccttcctgca gggccgcctg
     5701 gtcaaaggtg tagaggcgaa gttcgcccgc gctgatcgcc gggaacacaa acgagccgtc
     5761 gtcgagcgtc gtggcgatcg cgatggcgca ttcgctcggg acggcgcccg acagcgatcc
     5821 gggcgggggg ctgcaacgca cgccgggctg cgactcgttc cggtagtagg ccaccacctg
     5881 cacgcccggc acgcgcgtcg tgccgtccga tcgcaggacc cggcccgaca cgctcccggt
     5941 cttcaccgtg atctgcgtcg tgctgacgtc gagcaggacg agatcccggg tgaccgtcgc
     6001 accggcgctc gggatgtagt cgctgaggaa caccgacgcg ggccgggcgg tgttcacggc
     6061 ctccacgagg atgttgccca ccgggatccg ccggatctcg aaggccccgg ccgcgtcgga
     6121 cgtcgcgccg tactggctct cgtcggtgag gctcgtcacg cgcaggctcg tgttcgcgag
     6181 cggcgtcacc ccatcctccg cgagcgtccg gcccgcgaag gccccgcggc ccaagaacac
     6241 catgttgagg ttgagcagct ggccgtcccg ggacaccgtg aaccgctgcg tccgcaggtc
     6301 gtcggcctcg gtgttgatgg ccaccatctt cacggcggcc accggcagcg acacatagtc
     6361 gaactggtac cggccctcgg cgtcggcgaa gtcggaggcg atgccgacga ccacggtgcc
     6421 ttcttcgtcg cagcggaact cgtagaacag ccgcacctcg gcgaactgcg ccggcgtgcc
     6481 gtccgcgttc aggaccttgc ccgagacgac gccgccggcg tcggtgacgg tcgcctggat
     6541 gggcaccgag gccgaggcaa ggacgttccc ccgggtatcg gccacgcccg tgaccgtgat
     6601 cgtccggtcc acgaagcggc cgaccggttc gcgcagggcg acgtaggcga tccggttccc
     6661 gggctgcagc gccacgtcga gcacgcggtt cccttcgacc gcgaacgcgg tgataccctg
     6721 gtccgtgact gcgtcctgca cgctctcggg cgtgacttcc tcgctgaaca gcacggcgat
     6781 gacgcgtcct gcgtgcacgc gccggttctc gaggccgatg gccgagcacc cgacgacatc
     6841 ggcctccgcc atctgcacgg cgccgagcac cgagggcgga gggtcggtaa ccgccgcgcc
     6901 tgccgcctcg cgcgcctgcg ggctggcagc gccggcgagc gagaaggtga cgaggtacgg
     6961 atcgccggcc ggctgctgca gcgccggaac ctccgcgggg ccaaggtccg ggaacgacgc
     7021 gaatcgcagg cggaggtcgg cccctggata cgcgacgctc acgccgtaca gcgcacccgg
     7081 cgccgcgccg gccttgcgcg acagcgtcac gtggaacgcg ccgggctcgg ggacggcgac
     7141 gagcagcagc cgggccgtca ccgtgtcgcc gttccggaac gtgaggacgt cgccgtacgg
     7201 gatctccttg acggtgcgcc ccgcgtccag cccgcccagc ctccgtcctg ctgcgtcggt
     7261 cagcaccgcg tcgaccggca cgtcgctggc gccgtcgccg gcgacgagga tggacaggtg
     7321 ccccgggcgc gaggtgaact gcgccgcgat ggcctggtgg aacggcgcgg cgcctgcggc
     7381 gccaagccat ggcagcaccg cggaggccac ctgatcggcg agcgcatccc cgcggacgga
     7441 cttgcggcgc agcaggtcga agccgcggac gtccgcctgg agcagcgaca gcatctccgg
     7501 cggatctgtg tcctcgaccc gctcgcccag ccgcgagtac tcggagccga gcatgtccat
     7561 ccacaggtcg gcgaccgcct gcgggacggg cgcgccaagc aggatccgga accccgcctc
     7621 ggccgtctcg acggcccggc cgaggacgat ctgcttcgag aaccgctcga gatccttcgg
     7681 gaagacgatc ggcggcgcgg tggcgaccgc ccacgcgcgg ccgagcagtc cgagcgccgc
     7741 gtcgcgcacc gccttcggca gcgagccggc ttccttcggc agcacgagcg agtcgggcga
     7801 gagcggcacg cccagctcgc cgacggcggt cttcagcgcg aaccgcccct ccacctgctc
     7861 cgccgagtcc agcgtcgcgg ccgtgacgat gcccgtccgc tgggagatca gcttgaaggt
     7921 catcgtagcc gagtcgtcgg gcgcgatgta ctccacgctc ttggtcggct cgtcgtccag
     7981 ggcggcgccg ctgatgttat gggaatagag gttcacgctc acgaagttcg ccggcgcccc
     8041 tgacgtgttc gtgacggtga cgtccagcgt gtagggctcg ccggcattga cgacgtccgg
     8101 gtgcgtgaag gtgagcgtga acttcgggtt gcggacgagc accacgccgg ccgcccggcc
     8161 gcggacgggc accgggccga cgggcatgcc gttcagcgtg cccgtcagtt ccatctcgac
     8221 gacccaggtg ccctcgcgcc ggccctcgac caggtactcc gcgttgcccg tctcgccggg
     8281 cccgagggtc gtaatgtcgt cgcccgtccc gagcttcccg tccgcgccgg cctgcgtcac
     8341 cctttgatgc ggcggcgact cgcccgtcgc ggtctgcgcc atgcgcagcg ggtcgtcggc
     8401 cgaccccgcg acct
//
LOCUS       DAOSIF010000074         8227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8119_length_8227_cov_3.966226, whole genome shotgun sequence.
ACCESSION   DAOSIF010000074 DAOSIF010000000
VERSION     DAOSIF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8119_length_8227_cov_3.966226"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PLT35_04025"
     CDS             <1..1031
                     /locus_tag="PLT35_04025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28424.1"
                     /translation="GPAPAGRARYHSLAPPLGVRHLRLLRAGAVRTFARRANADVVIE
                     RYHNFGGEGVRAARAIGAAAVLEVNAPIIDYPGSWKARLDKAALVEPMRRWRDWQCRH
                     ADLIVTPAAEVLPPFVPRARILETEWGADTDAFAPGASGPVPFARRRGAVLAVFAGAF
                     RPWHGAVHLVRAVRQLRLEGQLDIDAVLVGEGPELAPARKEAAGLDGVTFTGPVAHAE
                     MPACLAAADLGVAPFDVAAHPPLQLGFYWSPLKVFEYMASGLPVVAPAIPRLAGILGP
                     DLASGLYDPNAPRGLADALLRLAANRSERERMGKVGRERACREFSWSAHCRRLEEAIA
                     PLARRRTA"
     gene            1031..2290
                     /locus_tag="PLT35_04030"
     CDS             1031..2290
                     /locus_tag="PLT35_04030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28425.1"
                     /translation="MRVLLATDAFPPVCGGSGWSTYELARGLRQAGHEVGVVQVVGER
                     PRRDGETYDGFRIRIVGIGAPAVPFVRNYWRNERLWRRAAVGIGRAAAAHGAEIIHAQ
                     HVFSSPAAVEAAAAARVPAVCTVRDYWPVCYWGTLILDPASTGLCPGCSARMMTACLR
                     PRAGAAWPATLPAVPYMRANLARKQRALARADAIVAVGSVIARDLRERAPLVAGRRLE
                     VIPNPVNVSALRREAGAAPLKGPYAVFVGKLEANKGADALLPAVERARLPWPLAVVGD
                     GALRPRLEAEARRLSRDARFLGWVERERAMAWLSHASLLVFPSRGPESLSRVLLEAAA
                     VGVPIAAMNTGGTGDIVRHGVTGLLSETPEGLGDDVARLVSDRGLAADLARRAREHVE
                     SRFDTPVVVERLLALYRDLIDRRRRDG"
     gene            2283..3548
                     /locus_tag="PLT35_04035"
     CDS             2283..3548
                     /locus_tag="PLT35_04035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28426.1"
                     /translation="MGDATGAAAGLRLAVLTRAIAPLHGVGGLERATQDLVRHLLDRG
                     AAVTVLTRPPTAAAPWSQPGLSFRFVPYVTFPFAGRRGTTILDRSTAYPVFGFRLGRL
                     AARAVRAREVDLVYGLGASTLGYAIARRRAPGATAPLVMNPQGLEEFGGPDGLYGGRP
                     SKRLGYGPLRAAVRYCAAAADRVIATDRSLEPLIARTLGVAADRVSLVPNAVDLASCD
                     ALASPRDGQRIRQARAIGPAELVLLSVARIERNKGLHVLADALASLADLPWRWVVVGD
                     GPFRGALEARIAERGIGARTLLAGRVDDATLHAWYEAASLFVHPTQYEGSSIVTLEAM
                     AHRLPVVASAAGGLPDKVRPGETGWLVPPGDAAGLAGALRAAMHPGAPLEAMGRAGRA
                     LVEAEFSWARSADRMLAVFGAIMREATIT"
     gene            3545..5089
                     /locus_tag="PLT35_04040"
     CDS             3545..5089
                     /locus_tag="PLT35_04040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG28427.1"
                     /translation="MRHPWKQDAAAAARPGLALALILAAGFALRVVNLGSGIPFAVGV
                     DEPAIMTTVVRILKSGDFNPRFFEYPTGYIYVQTGVAIVNFLVGAMRHSWKAVEQVAP
                     ADFYHWSRAVTAILGTATIALLYRAGVRWGTRPALVASALLAVMPMHVRESHFVLTDV
                     PMTFAAVLAFLLSLRAAERPAPAAFALAGAAAGLAAGIKYNGLMAVVMPLAAVAAAGP
                     SWGRRAAALAAASAACVAAFFVTTPYALLDLPAFLNGFGIQADAFSGRALSGEPSWLL
                     YAKHLRLAFGWPAAMLAVAGLVLACVRAAAGPDRLRWIHLVVFPAAYFWLITGWGLLY
                     ARYALPLVPFLALMAGIAAVALLERLDRLPVRPAIRVAAALALLLAAIAPGSLRSADW
                     VRQHGVETTQALAWKWVSRHVWPGSMIVSEARGFELPQPPYRTESVPSLAGCDPAELA
                     AAGVEWLVLSSDAWGASRSRGRTPPGSPPAEYAAILSQASDVRVFAPTPQHPGPHLRL
                     VRIARR"
     gene            complement(5139..>5549)
                     /locus_tag="PLT35_04045"
     CDS             complement(5139..>5549)
                     /locus_tag="PLT35_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28428.1"
                     /translation="EREKRKGGAVEQARVAREMAAIKGRFASEAAFEAELERLGLGGG
                     RAMAFVRETLEARRYVDRRFGASVEPSEEELRDYYARHLERFVRDGRQLDFVEAADDV
                     RTALQNERREQAVATWLDRLRRRSSVVEIYRAPR"
     assembly_gap    5550..5559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5560..5854)
                     /locus_tag="PLT35_04050"
     CDS             complement(<5560..5854)
                     /locus_tag="PLT35_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28429.1"
                     /translation="MKRAAGTKQPVSRAAVAALALSLAWAPLGAAELLDRVLAVVSGT
                     VITLSDARASLAFGFVGAEPAGDRIAAAVRWLIDRQLVLDEVLRSGGEEVDQAR"
     gene            complement(6034..>8227)
                     /locus_tag="PLT35_04055"
     CDS             complement(6034..>8227)
                     /locus_tag="PLT35_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOG28430.1"
                     /translation="IDALVEELSEGRGVAVSIDVHRGRRVSVEFEGDPLPPPQLAELV
                     PIQREASVDEDLLEDSRARIVSYLRGRGYRDARAEFRRVVEDSRLRIVFTVANGPLYR
                     VAGIVIDGASQVPLKELTPLLRLSPGQAYIQAVVDGDLAALTAAYRRRGFGEASCKAA
                     IEPVPNARTAHEAPVLVTIRIAEGARTLVGSVGVSGNEAVADADLLAGLSTRPGQPFY
                     APNTEADRDRILLRYLNLGYRLARVEASVEHDPDTGRASVVFPVREGPQILVGHILVV
                     GNARIGMDTIRRELAVEPGRPLALDAVNESQRRLASLGLFRRVTISEIQQRDDHVRDV
                     VVTVEEAPATTLGYGVGLEAQKVETSEIAPRGFVEIGRRNLFGGNRSINLFGRVSLRR
                     RTAEGAATGGQPVDGGSETAAEYRVAATYREPRLWRSLDFQIIAGAERGRRTSFSFEH
                     QSARFNLSRRLDAAWSVQGQYSLQENRIYHDRIAPADRPLIDRLFPQVRIASVSASAV
                     RNTRDDVFDPGRGTFLGLNGELALRELGSEVGFAKTFLQGFIYRRLPTSRRIVLAGGL
                     RLGLGVGFPREVRLTDEHGEPVIGDDGGPQIVRVSDLPASERFFAGGDTTVRGYRLDH
                     VGRPDTLDSDGTPKGGRAGLILNGEVRVGLWRDLGVVGFIDAGNAFRVVDDVTLRHLR
                     SGAGFGIRYKSPIGPFRLDLGFKLGKLRKFVGTREGRRAVHISIGQAF"
BASE COUNT          985 a         3021 c         2983 g         1228 t
ORIGIN      
        1 ccggtcctgc gcccgccggg cgcgcgcgct accactccct ggcaccgccg ctcggcgtcc
       61 gccacctccg gctgctccgc gccggggctg tccgtacgtt cgccaggcgg gccaacgcgg
      121 acgtggtcat cgagcgttac cacaacttcg gcggtgaggg cgtgcgcgcg gcgcgagcga
      181 ttggagcggc cgccgtactc gaggtcaacg cgccgatcat cgactacccg gggtcgtgga
      241 aggccaggct cgacaaggcc gctctcgtcg agcccatgcg cagatggcgc gactggcagt
      301 gccgccacgc cgacctcatc gtcacgccgg cggcggaggt gctgccgccg ttcgtgccgc
      361 gggcgaggat cctcgagacc gaatggggcg ccgacacgga cgccttcgcg ccgggcgcct
      421 ccggccccgt gccgttcgcc cggcggcggg gcgcggtact ggcggtcttc gccggcgcct
      481 tcaggccctg gcacggcgcg gtgcaccttg tgcgggccgt gcggcagctc cgcctggagg
      541 gccagctcga catcgacgcg gtgcttgtcg gcgagggtcc cgaactcgcg ccggccagga
      601 aggaggcggc gggcctcgac ggcgtcactt tcacgggccc ggtggcccac gccgaaatgc
      661 cggcgtgcct ggctgcggcc gatcttggcg tcgccccgtt cgacgtcgcg gcgcacccgc
      721 cgctgcagct gggcttctat tggtctccgc tgaaggtgtt cgagtacatg gcgtccggcc
      781 tcccggtggt ggcgccggcc atccctcgcc tggccgggat tctcggaccc gacctcgcaa
      841 gcggactcta cgatccgaac gcgccgcggg ggctggcgga cgcgctgctg cgcctggcgg
      901 cgaaccgctc ggagcgggag cgcatgggga aggtcggccg cgaacgggcc tgccgcgagt
      961 tcagctggtc ggcgcactgc cgccgcctcg aggaggcgat cgcgccgctt gcgcggcgtc
     1021 ggacggcgtg atgcgagtcc tgctggcgac cgacgcgttt ccccccgtct gcggcggcag
     1081 cggctggagc acgtacgagc ttgcgagagg cctccggcag gccggacacg aggtgggcgt
     1141 cgtgcaggtc gttggggaac gcccgcgccg tgacggcgag acctacgacg gtttccgcat
     1201 ccggatcgtc ggcatcgggg cgcctgccgt gccgtttgtg cgcaactact ggcggaacga
     1261 gcggctgtgg cgccgtgcgg cggtcggcat cgggcgggcg gcggccgcgc acggcgccga
     1321 gatcatccac gcgcagcacg tgttctcctc tcccgccgcc gtcgaagccg cggccgcagc
     1381 ccgcgtgccg gcggtctgca cggtgcggga ctactggccc gtctgctact ggggcacgct
     1441 catcctcgac cccgcctcga cgggcctgtg ccccgggtgc tcggcgcgca tgatgacggc
     1501 gtgtctccgg ccgcgggccg gggccgcgtg gcctgccacg cttccggccg tgccgtacat
     1561 gcgcgcgaac ctcgcccgca agcagcgcgc ccttgcgcgg gctgacgcga ttgtggccgt
     1621 cggctcggtc atcgcgcggg accttcgcga acgcgcgccg ctcgtggcag ggcggcgcct
     1681 cgaggtcatt cccaatccgg tgaacgtctc cgctctgcgc cgcgaggccg gcgccgcgcc
     1741 cctgaagggc ccctacgccg tgttcgtcgg gaagctggag gcgaacaagg gcgccgacgc
     1801 gctgctgccg gcggtcgagc gcgcccgcct gccgtggccg ctcgccgtcg ttggcgacgg
     1861 ggccctgcgg ccgaggctgg aggccgaagc aaggcggctg agccgggatg cgaggtttct
     1921 cggatgggtc gagcgcgagc gcgcgatggc ctggctgtcg cacgcctccc tgctcgtctt
     1981 cccctcccga gggcccgagt cgctgagccg cgtgctgctc gaggccgcgg cggtgggcgt
     2041 gccgattgcg gccatgaaca cgggcggaac cggggacatc gtccgccacg gcgtgaccgg
     2101 gctcctctcg gagacgccgg aggggctcgg cgacgacgtc gcccgcctgg tgtcggaccg
     2161 cggccttgca gcggatctgg cgcggcgcgc ccgcgagcac gtcgagtcgc gattcgacac
     2221 gccggtcgtg gtcgagcgcc tgctcgcgct gtatcgggac ctcatcgatc ggaggcgccg
     2281 cgatgggtga cgcgaccggc gccgcggccg ggctccgtct cgccgtgctc acccgcgcga
     2341 tcgcgccgct tcacggcgtc ggcggcctcg agcgcgccac gcaggatctc gtccggcacc
     2401 tgctggatcg cggcgccgcg gtgacggtcc tgacgcggcc gccgacggcg gcggcgccgt
     2461 ggtcgcagcc cggcctctcc ttccgcttcg tgccctacgt gacgttcccg ttcgccggca
     2521 ggcgcggcac gacgatcctg gatcgatcca ccgcctaccc cgtgttcggc ttccgcctgg
     2581 gtcgcctggc ggctcgagcc gtccgtgcgc gcgaggtgga tctcgtctac ggcctcggcg
     2641 cgagcacgct cgggtatgcg atcgccaggc gacgtgcgcc cggagcgacc gcgccgctcg
     2701 tcatgaaccc gcagggcctc gaggagttcg gcgggcccga cggcctgtac gggggccggc
     2761 cgtccaagcg cctgggctac ggcccgctgc gcgcggcggt gcggtactgc gccgccgcgg
     2821 ccgatcgcgt cattgccacg gaccggtcgc tcgagccgct catcgcgcgc acgctgggcg
     2881 tggcagcgga ccgggtgagc ctggtgccca acgccgtgga cctcgcctcg tgcgacgcgc
     2941 tcgcgtcgcc tcgggacggg cagcggatac ggcaggctcg cgccatcggg cccgccgaac
     3001 tggtgctgct gagcgtggcg cgaatcgagc gcaacaaggg gctgcacgtg ctcgccgatg
     3061 ccctcgcgtc gctcgcggac cttccgtggc gctgggtggt cgtcggcgac ggcccgttca
     3121 ggggcgcgct cgaggccagg atcgcggagc gcggcatcgg ggcgcgcacg ctcctggccg
     3181 ggcgggtgga cgacgcgacg ctccacgcct ggtacgaggc ggcctccctg ttcgtgcatc
     3241 cgacgcagta cgagggcagc tcgatcgtca ccctcgaggc catggcccac cggctgccgg
     3301 tcgtggcctc ggctgccggc gggctgccgg acaaagtccg gccgggtgag accgggtggc
     3361 tcgtgccgcc cggcgacgcg gccgggctcg ctggcgccct ccgggcggcc atgcacccgg
     3421 gcgcacccct cgaagcgatg ggccgcgcgg ggagggcgct cgtggaagcg gagttctcat
     3481 gggcgcggtc cgccgaccgc atgctggcgg tcttcggcgc gatcatgcgg gaggcgacga
     3541 tcacatgaga cacccatgga agcaggacgc cgcggcggcc gcgcggccgg gtctggcgct
     3601 cgcgctcatc ctcgccgccg ggttcgcact gcgcgtcgtg aacctcggga gcgggattcc
     3661 cttcgccgtc ggggtggacg agcccgccat catgacgacg gtggtgcgca tcctgaagtc
     3721 cggcgacttc aatccgaggt tcttcgagta cccgaccggc tacatctacg tgcagacggg
     3781 cgtggcgatt gtgaacttcc tcgtcggcgc gatgcggcac tcgtggaaag ccgtcgagca
     3841 ggttgcgccc gccgacttct accactggag ccgggccgtc accgcgatcc tcggcacggc
     3901 gacgattgcg ctgctttacc gcgccggcgt ccggtggggc acgaggccgg cactcgtcgc
     3961 ttccgcgctc ctcgccgtca tgccgatgca cgtgcgtgag tcgcacttcg tgttgacgga
     4021 cgtgcccatg accttcgccg ccgtcctcgc cttcctgctg tcgctccgtg cggcggagcg
     4081 gccggctccg gcggcgttcg cgctggcggg ggcggccgcg gggctggcgg cgggcatcaa
     4141 gtacaacggc ttgatggcgg ttgtcatgcc gctcgccgcg gttgcggccg ccggcccgtc
     4201 ctggggacgt cgggccgcgg cgctggctgc ggcgtcggcc gcgtgcgtgg cggcgttctt
     4261 cgtcaccacg ccctacgcgc tgctcgacct gccggcgttc ctgaacgggt tcggcatcca
     4321 ggcggatgcg ttttcgggcc gcgccctgtc gggggaaccg agctggctcc tgtacgcgaa
     4381 gcacctgcga ctcgccttcg gctggccggc cgcaatgctt gccgtcgccg gtctcgtcct
     4441 ggcgtgcgtc cgcgcggcgg cggggccgga ccggctccgc tggatccacc tggtggtctt
     4501 ccccgcggcg tacttctggc tgatcaccgg atggggactg ttgtatgcgc gctacgccct
     4561 gccgctggtg ccgttcctcg cgctcatggc gggcatcgcg gccgtcgcgc tcctcgagcg
     4621 tctcgaccgc ctgccggttc ggccggcgat ccgggttgct gccgccctgg ccctgctgct
     4681 cgcggcgatt gcgcccggct cgctgcgatc ggcggactgg gtgcggcagc acggcgtcga
     4741 gaccacgcag gcgctggcgt ggaaatgggt gagccggcat gtctggcctg gctcgatgat
     4801 cgtgtccgag gcgcgcggct tcgagctgcc gcagccgccc taccgcaccg agagcgtccc
     4861 gtcgctcgcc ggctgcgatc ccgccgaact cgctgctgcc ggcgttgagt ggctggtgct
     4921 gtcgtcggac gcgtggggtg cgagccgatc acggggccgg acgccaccgg gctccccgcc
     4981 ggcggagtac gccgccattc tgtcccaggc cagcgatgtc agggtgttcg cgccgacgcc
     5041 gcagcacccg gggccgcacc tgcgcctcgt ccggatcgcg cggcgctagt cggccggcgg
     5101 ccggccgcgc ctgcgccggc ggcgccgcac gggctctgtc agcgcggtgc tcgatagatc
     5161 tcgacgacgg aggagcggcg cctgagccga tcgagccacg tggcgacggc ctgctcgcgc
     5221 cgctcgttct ggagcgccgt gcgcacatcg tcggccgcct ccacgaagtc cagctggcgg
     5281 ccgtcgcgaa cgaaccgctc caggtggcgg gcgtagtagt cgcgaagctc ctcctcgctc
     5341 ggttcgaccg aggcgccgaa ccggcggtcg acgtatcgcc gcgcctcaag cgtctcgcgc
     5401 acgaaagcca tcgcgcgccc tccgcccagg ccgagccgtt ccagttcggc ttcgaacgcg
     5461 gcctcggacg caaagcgccc cttgatggca gccatctcgc gggccacgcg ggcctgctcg
     5521 accgcgccgc ccttgcgctt ttcacgctcn nnnnnnnnnc gcgggcctgg tccacctcct
     5581 cgccgccgct ccgcagcact tcgtccagga cgagctggcg atcgatcagc cagcgcacgg
     5641 cggctgcgat tcggtcgccg gctggttcgg cgccgacgaa accgaacgcc aggctcgccc
     5701 gggcgtccga cagcgtgatc acggtgcccg acacgacggc cagcacgcga tccaggagtt
     5761 ctgcggcgcc gaggggagcc caggcgaggg acagcgccag cgccgccacg gcggcgcggc
     5821 tcacgggctg cttcgttccc gccgctctct tcatcaccgc tgctctctcc gccaccgccg
     5881 ctcccatcgt gtccgccgct ctgctcacca ggcgcccgcg ccgctgcaat cctcgtcgtc
     5941 gcgcactgcg cgtggcgcac tccgcatcca ggatcccgag cgctcctcta cctcctgtct
     6001 tcgtccattc cgaatgctga attccgcctt cccctagaac gcctgcccga tgctgatatg
     6061 aaccgctcgc cggccctcgc gcgtgcccac gaacttccgg agcttcccca gcttgaatcc
     6121 gagatccagc cggaacggac caatgggtga cttgtaccga atgccgaatc ccgcgccgct
     6181 ccggaggtgc cggagcgtga cgtcgtccac gacgcgaaag gcgtttcccg cgtcgatgaa
     6241 cccgacgacc cccaggtccc gccagagccc cacgcggact tcgccgttga gaatcaggcc
     6301 ggcgcggccg cccttcggcg tgccgtcgct gtcgagcgtg tccgggcggc ccacgtgatc
     6361 cagccggtat ccgcgcaccg tcgtgtcgcc tccagcgaag aagcgctcgc tcgccggcag
     6421 gtcgctcacg cgcacgattt gcggcccccc gtcgtcgccg atcaccggct ccccgtgctc
     6481 gtcggtgaga cggacctcgc gcgggaagcc gacgccgagt ccgaggcgca atccgccggc
     6541 caggacgatg cggcgggagg tcggcagcct gcggtagatg aagccctgga ggaacgtctt
     6601 ggcgaagccg acctcggacc ccagttcacg gagggcgagc tcgccgttca agccgaggaa
     6661 cgtgccgcgc cccggatcga agacatcgtc gcgcgtgttg cggacggcgg acgccgagac
     6721 cgacgcgatc ctgacctgcg ggaacagccg gtcgatgagc gggcgatcgg ccggtgcgat
     6781 ccgatcgtgg tagattcggt tctcctggag cgagtactgg ccctggacgc tccaggcggc
     6841 gtccagccga cgggacaggt tgaagcgcgc ggactggtgc tcgaagctga aactcgtgcg
     6901 cctgccccgc tcggcgccgg cgatgatctg gaagtccagg gaccgccaga gcctgggctc
     6961 gcggtacgtc gccgccacgc ggtactccgc cgcggtctcg ctgccgccgt cgaccggctg
     7021 gccgccggtc gccgccccct cggcggtgcg gcggcggagg ctgacccggc cgaacaggtt
     7081 gatggaccga ttgccgccga acaggttccg ccggccgatc tcgacgaagc cgcgcggggc
     7141 gatttccgac gtctcgacct tctgcgcttc cagcccgacg ccgtatccca gcgtcgtcgc
     7201 gggcgcctcc tcgacggtga cgacgacgtc ccgcacgtgg tcgtcgcgct gctggatctc
     7261 ggagatcgtg acccggcgga acaggcccag cgacgcgagc ctccgctggc tctcgttgac
     7321 ggcatccagc gcgagcggcc ttccaggctc caccgcgagt tcccggcgga tcgtgtccat
     7381 gccaatgcgg gcgttgccga cgacgaggat gtggccgacg aggatctgcg ggccttccct
     7441 gaccgggaag acgaccgacg cccggcctgt gtcggggtcg tgttcgaccg acgcctcgac
     7501 gcgcgcgagg cggtagccga ggttcaggta gcggaggagg atgcgatcgc ggtcggcctc
     7561 ggtgttgggc gcgtagaacg gctgcccggg cctcgtcgag aggcccgcga ggaggtccgc
     7621 gtcggccacc gcctcgttgc ccgagacacc gacggaaccg accagcgtgc gcgcgccttc
     7681 ggcgatccgg atcgtcacga ggaccggggc ctcgtgggcg gtcctcgcgt tgggcacggg
     7741 ctcgatcgcg gccttgcacg acgcctcgcc gaacccgcgc cggcggtagg ccgcagtgag
     7801 cgccgcgagg tccccgtcga ccacggcctg gatgtacgcc tggcccggtg acaggcgcag
     7861 gagcggcgtg agctccttca gcgggacctg cgacgcgccg tcgatgacga tgcccgccac
     7921 gcggtagagc ggcccgttcg ctacggtgaa cacgatccgg agccggctgt cttcgacgac
     7981 ccgccggaac tccgcgcgcg cgtcccggta cccgcgcccc cgcaagtacg acacgatacg
     8041 ggccctggag tcctcgagga gatcctcgtc caccgacgcc tcgcgctgga tcggcacgag
     8101 ctcggccagc tgcggcggcg gcagagggtc gccctcgaac tcgaccgaca cccggcgccc
     8161 gcggtgaacg tcgatcgaca ccgcgacccc gcgtccctcg gacagctcct cgacgagcgc
     8221 gtcgatt
//
LOCUS       DAOSIF010000075         8212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8133_length_8212_cov_4.304646, whole genome shotgun sequence.
ACCESSION   DAOSIF010000075 DAOSIF010000000
VERSION     DAOSIF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8133_length_8212_cov_4.304646"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLT35_04060"
     CDS             <1..703
                     /locus_tag="PLT35_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28431.1"
                     /translation="VALAASACDRADFGKHYEYDEDITLAVDGSARVRVSASIPALVN
                     LRGMDLDARPTAAVDKAKVRALFASPAARVTRVSRWRRYGRQFVQVRMDVDDITKLGG
                     TPAFSWAAYQFARKDGLIVYRQALGAPAGKDAGAAGWKGDELVSIRVHLPSRIRFHNA
                     GAGNLRRGNILVWEQRLADRQAGKPLEAEARIEETSILYSTLVLFGASALLALAVFGA
                     FIGWIATRGRRRRGR"
     gene            complement(1000..1497)
                     /locus_tag="PLT35_04065"
     CDS             complement(1000..1497)
                     /locus_tag="PLT35_04065"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOG28432.1"
                     /translation="MSRGCTRLPLAVAAAALLAGPAAAAQKPAAAPWRQLTLTEAQRT
                     QLKGIQDQHAKDAQAVRERLRAARRQLADAMRPDIPDEAAVKAAAQAVAAAQTEQSVL
                     QARLKAQRMKVLTPEQLQQLTDARARAAERQARASERRARAADRQDRALGRRARPAAP
                     WRGRR"
     gene            complement(1630..2205)
                     /locus_tag="PLT35_04070"
     CDS             complement(1630..2205)
                     /locus_tag="PLT35_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOG28433.1"
                     /translation="MTTLVRRPIVRGVLLLCLGTLSPASAGPQEGWRDGQIFDRVAEA
                     VLTYPQYTIFDDVSADVANGFVTLAGKVTIEYKRKDLARRVAQVPGVKEVENAITVLP
                     ASAFDDDVRYAVARAIYGHASFWHYASMTNPPIHVIVERGRLTLTGVVANEVERSLAR
                     SIAASSNAFSVSIELKTDDEMKAILLRRRQR"
     gene            complement(2332..3075)
                     /locus_tag="PLT35_04075"
     CDS             complement(2332..3075)
                     /locus_tag="PLT35_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOG28434.1"
                     /translation="MPVLVIAIGLAVVGSIGGLLVAATLLLFKDRVRVRLVPWLVSFA
                     VGALLAVALLDLLPEALEALSPAAAFGTLLAAILAFFALEKFLLWRHCHADHCEIHHA
                     AAPLILIGDAFHNFVDGAVIAAAVHVSVPLGVTTAVAVAAHEIPQEVGDFAILLGAGY
                     SRVKALWLNVLSGLSAVLGVLVAGLAIDRAPAVLPYFLPVAAASFLYVAMSDLIPSLH
                     RGPFVKGAFRQVLAVVAGILAIVAFAAHD"
     gene            3307..3897
                     /locus_tag="PLT35_04080"
     CDS             3307..3897
                     /locus_tag="PLT35_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG28435.1"
                     /translation="MAGILRFRRARGPVELAVDMAGVRLGERLIQAGVANPRVFAALA
                     GKAGLTGRACAVVDAAEAGARLEAAAAREGVLVEVSVAAHGRWPYEDASFDVGVLDGN
                     ALLRAGEGDRLDRLNDMRRVVRPGGRVIAVRSWPAGLALRLGFIAERREPSGEAAALR
                     EAMEAAGFRPVRLLAEREGLTFVEGFRPSDDAGAGG"
     gene            4012..5325
                     /locus_tag="PLT35_04085"
     CDS             4012..5325
                     /locus_tag="PLT35_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOG28436.1"
                     /translation="MKKPIAIAPAAGKLGVMCVGLGAVATTFIAGVLAARKGIASPIG
                     SMTQMGTIRLGKRTEGRSPLVRDFVPLADLNDLVFAGWDIFEDDCYESARHAGVLEAS
                     LLDQIKPELEAIKPLKAVFDRQYVKRLDGPNVKKGRNKRDLAEQLREDIRAFKRDNGL
                     SRLVMIWCASTEVYLSETPAHQSVEAFEKALEADDSSIPSSMIYAYAALMEGVPFANG
                     APNLTVDVPAMVALARKNKVPISGKDFKTGQTLLKTVIAPGLKARLLGVKGWYSTNIL
                     GNRDGEVLDDPESFKTKEESKKSVLDYILQPDLYPELYSDICHVVRINYYPPRGDNKE
                     GWDNIDMFGWLGYPMQLKVNFLCRDSILAAPIVLDLVIFMDLAKRAGWSGIQEWLSFF
                     YKSPMHAKGLYPEHDLFIQSMKLKNTLRYLMGEDLITHLGAEYYD"
     gene            5434..5958
                     /locus_tag="PLT35_04090"
     CDS             5434..5958
                     /locus_tag="PLT35_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28437.1"
                     /translation="MNSKAMLKPALAGGLVMGVLSGLPGVSLGNCCCCAWLVAGGIVA
                     AYLLQTWTADPITLGDGALVGLLAGIFGAVVSVVVSVPMTQLLGPFQQQLVQRIAESQ
                     QGLPPEVQEALDNFGAQGLSIVRLLLSFVMLLVLGAIFSSLGGLLGAFFFKKKVAPAP
                     PPFTEGPAGPIIDA"
     gene            complement(6098..6907)
                     /locus_tag="PLT35_04095"
     CDS             complement(6098..6907)
                     /locus_tag="PLT35_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28438.1"
                     /translation="MGLSSYQRDVCRLIAANRRRSGESYFAGGATLNELIAAPRLSRD
                     IDVFHDTEEALQASWHADRLDLERAGYAVRTVRERPGFVEAEVRRGPETVLMEWARDS
                     AFRFFPLVEHDELGLVLHPFDLATSKVLALVGRVEPRDFLDVVNCDREVQPLGYLAWA
                     ACGKDPGFSPAAILEEAARASRYTAEDFQGLDFEGGAPDPGELMRAWHALLGAARFVV
                     NVLPSESAGRAVLDRAGRLFRGDVDALEAAVASGELRYHSGSIRGALPRIV"
     gene            complement(6865..7095)
                     /locus_tag="PLT35_04100"
     CDS             complement(6865..7095)
                     /locus_tag="PLT35_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28439.1"
                     /translation="MSTADQEREAALRALVDEYRDRCLWFLEKGYYPVTVAERLRVLD
                     SLQRHGDLSAFQRAGELKRWVSAAISETSAGS"
     gene            complement(7103..7528)
                     /locus_tag="PLT35_04105"
     CDS             complement(7103..7528)
                     /locus_tag="PLT35_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28440.1"
                     /translation="MVSAFVASALVTAITRRSRAAAPVGVAFQGSAFSAPVSRHWTRQ
                     LIVVAQVTLAMALLCNALLFARSVAAVYRVDVGYDTKNLVEAAVDVVAVHLTDGERIG
                     LYDEIEQALRRSLPSARAGRRGASAPGRMRTPRAAPTRV"
     gene            7568..7912
                     /locus_tag="PLT35_04110"
     CDS             7568..7912
                     /locus_tag="PLT35_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOG28441.1"
                     /translation="MIEDRARSRREDGRRPRQSVGDGKREYGPGANLARVVDPARLKA
                     ALRVPETQAKDVQLGQRAAIDTRTGPITGRVARVDPASTGGTVTVDVVLDGTMPKGAR
                     PDLTIDGTMTCR"
     gene            7951..8145
                     /locus_tag="PLT35_04115"
     CDS             7951..8145
                     /locus_tag="PLT35_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG28442.1"
                     /translation="MAAALERVEMAHRAKHLPGQLSGGQQQRVAVARAVVGKAAILPP
                     SVPCPLPLVPCPCAADTPVS"
BASE COUNT         1214 a         2876 c         2997 g         1125 t
ORIGIN      
        1 ggtcgcgctg gcggcatcgg cgtgcgaccg ggcggatttc gggaagcact acgagtacga
       61 cgaggacatc acgctcgcgg tggacgggag cgcgagggtc cgcgtcagcg cctcgatccc
      121 ggccctggtc aacctgcgcg gcatggatct cgacgctcgc ccgacggcgg ccgtcgacaa
      181 ggcgaaggtg cgcgcgctgt ttgcgtcgcc ggccgcgcgc gtcacgcgcg tcagccggtg
      241 gcggcggtac gggcgccagt tcgtgcaggt gcggatggac gtggacgaca tcacgaagct
      301 gggcgggacg ccggcgttct cgtgggcggc ctaccagttc gcgcgcaagg acgggctgat
      361 cgtctaccgc caggcgctgg gcgcgccggc gggcaaggac gctggcgcgg cgggctggaa
      421 gggcgacgag ctcgtctcga tccgcgtgca cctgccgagc cgcatccgct tccacaacgc
      481 cggcgcgggc aacctgcggc gcggcaacat cctcgtgtgg gagcagcggc tggccgatcg
      541 gcaggcggga aagcccctcg aggccgaggc gcgcatcgag gagacgtcga tcctctactc
      601 cacgctcgtg ctgttcggcg cgagcgcgct gttagcgctc gccgtcttcg gcgccttcat
      661 cggctggatc gcgaccaggg ggcgccggag gcgcggccgc tgagcgcggc ccgggtcggc
      721 ggcagtacgc ccgccggggc cgccccccgc agacccgctc acgcaggccg gcaacacgca
      781 tctccggggt tcggtcggcg gaacgcgggg ccccccttcg gcaccccacg aggacgtcgg
      841 cccctccgat gcgttttgcg agctgccgcc gagcggcctg gttcgagggt ctgcgggagg
      901 cggtcccggc gggctggagg cggcgcgtgc ccgggtgact ggcggctgga cccgtccccg
      961 cgtgccgccg gcggcgcggc gagcctcagc acgcgccgtt cagcgccggc cgcgccaggg
     1021 ggccgccggc cgggcccggc gcccgagggc ccgatcctgc cggtcggccg cgcgcgcgcg
     1081 gcgctccgag gcgcgcgcct gccgctccgc cgccctggcc cgcgcgtcgg tcaactgctg
     1141 cagctgctcg ggcgtgagca ccttcatgcg ctgcgccttc agccgcgcct gcagcacgct
     1201 ctgctctgtc tgcgccgccg caaccgcctg ggccgcggcc ttcacggccg cttcgtccgg
     1261 gatgtcgggg cgcatcgcgt cggcgagctg ccggcgcgcg gccctgaggc gctcgcgcac
     1321 ggcctgcgcg tccttggcgt gctgatcctg aattcctttc agctgcgtcc gctgcgcctc
     1381 ggtcagcgtc agctgcctcc acggcgcggc ggccggcttt tgcgccgcag cggcgggccc
     1441 cgccaacaga gcggcagccg cgacggccag cggcaatctg gtgcatccac gcgacatggc
     1501 aacctccggt gcttgcggcg ccggccggcg cgggagcggg ccggcgcttc tgtctcgttc
     1561 acgggattgg acaggcggcg cgggccgcgc gttacatgcc cgcccgcgcg cggagggccg
     1621 ggccgcgcgt cagcgctggc ggcggcggag caggatcgcc ttcatctcgt cgtcggtctt
     1681 cagctcgatg ctcaccgaga aggcgttcga cgaggcggca atcgatcgcg ccaggctgcg
     1741 ctccacctcg ttcgccacca cgcctgtcag cgtgaggcgg ccgcgctcga cgatgacatg
     1801 gatcggaggg ttggtcatcg aggcgtagtg ccagaacgag gcgtggccgt agatggcgcg
     1861 cgccaccgcg tagcgcacgt catcgtcgaa ggcggaggcc ggcagcacgg tgatcgcgtt
     1921 ctcgacttcc ttgacgccgg gcacctgcgc cacgcgccgg gccaggtcct tccgcttgta
     1981 ctcaatcgtc accttgcccg cgagcgtcac gaacccgttg gccacgtcgg cgctcacatc
     2041 gtcgaagatc gtgtactggg ggtaggtcag cacggcttcc gccacgcgat cgaagatctg
     2101 gccgtcccgc catccctctt gcggcccggc ggaggccggc gagagggtcc cgaggcacag
     2161 gagcaggacg ccgcgaacga tcggccgccg aacgagcgtt gtcatactcc tccagcgatc
     2221 aagcttcgtg ccatccgccg atccgcgaga actcgcggga acttgcgagt ccgccggttc
     2281 ctgcgaccgc cggagaggac ggcgcggacg cgccgcagcg accgggccgc gtcagtcgtg
     2341 cgccgcgaag gccacgatcg cgagaatgcc cgccacgacg gccagcacct gccgaaacgc
     2401 gcctttcacg aacggtcccc ggtgcaggct cggaatcagg tccgacatgg ccacgtagag
     2461 gaagctcgcc gcggccaccg ggaggaagta cggcagcacc gccggcgccc ggtcgatggc
     2521 caggccggcg acgagcacgc ccagcacggc cgagagcccc gacagcacgt tcagccacag
     2581 cgctttgacg cggctgtagc ccgcgcccag gaggatggcg aagtcgccca cctcctgggg
     2641 gatttcgtgc gcggcgacgg ccactgccgt cgtcacgccc agcggcaccg acacgtggac
     2701 ggccgccgcg atgacggcgc cgtcgacgaa gttgtggaag gcgtcgccga tgaggatgag
     2761 cggcgcggcc gcgtggtgga tctcgcagtg atcggcgtgg cagtgccgcc agagcaggaa
     2821 cttctcgagc gcgaagaacg ccaggatcgc ggcgagcagc gtgccgaagg cggccgccgg
     2881 cgagagcgcc tcgagggcct ccgggagcag gtcgaggagc gcgacggcca gcagggcgcc
     2941 gaccgcgaag ctgacgagcc agggcacgag ccggacgcgc acgcggtctt tgaagagcag
     3001 cagcgtcgcg gcaacgagca gcccgccgat gctgccgaca acggccaggc cgatggcgat
     3061 gacgagtacg ggcacggggg catcgtatca aatgcgcaag gcggacggcg ggaggcggac
     3121 ggcggaaggc acgggccaga aggctggagc cggcacccgc cgccgcggcg gcgcgccgga
     3181 ttctcgcacc ggccggccgg gatcgggcca taatcagccg tggacgaacg cgccggcccg
     3241 cgcgagcgga gccgcgcggg ggcgcgtccg gacgccccgc tcgacgcgca gtgtgtgagg
     3301 ccgactatgg caggcatctt gagattccgc cgcgcgcgcg ggccggtcga gctggccgtg
     3361 gacatggccg gcgtgcggct cggcgagcgc ctcatccagg ccggcgtcgc caacccgcgg
     3421 gtgttcgcgg cgctggccgg caaggccggc ctgaccgggc gcgcgtgcgc cgtcgtggac
     3481 gcggccgagg ccggcgcccg gctcgaggcc gcggcggcca gggaaggcgt gctcgtcgag
     3541 gtgagcgtgg cggcccacgg gcggtggccg tacgaagacg ccagcttcga tgtgggcgtg
     3601 ctcgacggca acgcgctgct gcgcgcgggc gaaggcgacc ggctcgatcg gctgaacgac
     3661 atgcggcgcg tcgtccgccc gggaggccgg gtgatcgccg tgcgaagctg gcccgccggc
     3721 ctggcgctgc gcctggggtt catcgccgag cggagggagc cgagcggcga ggcagcggcg
     3781 ctgcgggagg cgatggaggc cgccggcttc cggccggtgc ggctgctggc cgagcgcgag
     3841 gggctcacct tcgtggaggg cttccggcca tcggacgacg caggggccgg gggctaggcg
     3901 ctgggagccg gtgggacgcg gctgccgccg cgtcgttgga gcccgcagcg tgacattcga
     3961 gctggccagc gcctggcgcc tggtcccaaa cccctgatat agtgcgttcc cgtgaagaag
     4021 cccatcgcca tcgctccagc cgcaggcaag ctcggcgtga tgtgcgtcgg cctcggggcc
     4081 gtcgccacca cgttcatcgc cggcgtcctc gccgcccgca agggcatcgc gagccccatc
     4141 gggtcgatga cccagatggg cacgatccgc ctcggcaagc ggaccgaagg ccggtctccg
     4201 ctggtccggg acttcgtgcc gctggccgac ctgaacgatc tcgtgttcgc cggctgggac
     4261 atcttcgagg acgactgcta cgagtcggcc cggcatgccg gcgtcctcga ggcctcgctg
     4321 ctcgaccaga tcaagcccga gctcgaagcc atcaagccgc tgaaggccgt gttcgaccgc
     4381 cagtacgtca agcggctgga cggcccgaac gtgaagaagg gcaggaacaa gcgcgacctc
     4441 gccgagcagc tccgcgagga catccgggcg ttcaagcggg acaacggcct gtcgcgcctg
     4501 gtgatgatct ggtgcgccag caccgaggtc tacctcagcg agacgccggc ccaccagtcg
     4561 gtggaggcct tcgagaaggc cctcgaagcc gacgactcgt ccatcccgtc ctccatgatc
     4621 tacgcctacg cggcgctgat ggagggcgtg ccgttcgcga acggcgcgcc gaacctgacg
     4681 gtggacgtgc cggcgatggt ggccctggcc aggaagaaca aggtgcccat ctcgggcaag
     4741 gacttcaaga ccggccagac gctgctgaag accgtcatcg cccccggcct caaggcccgc
     4801 ctcctcggcg tgaagggctg gtactcgacc aacatcctcg gcaatcgcga cggcgaggtg
     4861 ctcgacgacc cggagtcgtt caagacgaag gaagagagca agaagtcggt cctcgactac
     4921 atcctgcagc cggacctcta ccccgagctc tacagcgaca tctgccacgt cgtccggatc
     4981 aactactacc cgccgcgcgg cgacaacaaa gaaggctggg acaacatcga catgttcggc
     5041 tggctcggct acccgatgca gctgaaggtc aacttcctgt gccgcgacag catcctggcg
     5101 gcgccgatcg tcctggacct cgtcatcttc atggatctcg cgaagcgcgc gggctggagc
     5161 ggcatccagg agtggctctc gttcttctac aagagcccga tgcacgcgaa gggcctgtat
     5221 cccgagcacg acctcttcat ccagtcgatg aagctgaaga acacgcttcg ctacctgatg
     5281 ggcgaggacc tcatcacgca cctgggggcg gagtactacg actagacgcc ttcgctcgcc
     5341 atgcgagcta cggcgatccc agggggggac aagggaccag gcgccaggcg ctagtcccgg
     5401 caggccgtgt agcgaaggcg tgggggggca tccatgaact cgaaggcgat gctgaagccg
     5461 gcgctcgccg gcggcctggt gatgggcgtg ctgtcggggc tgcccggcgt gagcctcggc
     5521 aactgctgct gttgcgcctg gctcgtcgcc ggcggcatcg tggccgccta cctgctgcag
     5581 acgtggaccg ccgaccccat cacgctgggc gacggggcgc tggtcggcct cctcgccggg
     5641 atcttcggcg ccgtcgtgag cgtcgtcgtg tcggtgccga tgacgcagct gctcgggccg
     5701 ttccagcagc agctcgtgca gcgcatcgcc gagtcgcagc agggcctgcc gccggaagtc
     5761 caggaggccc tcgacaactt cggcgcgcag ggcctgtcga tcgttcggct gctcctgtcg
     5821 ttcgtcatgc tgctcgtgct cggggcgatc ttctcgagcc tcggcggcct gctcggcgcg
     5881 ttcttcttca agaagaaggt cgccccggcg ccgccgccct tcaccgaagg tccggccggc
     5941 ccgatcatcg acgcgtaggg cggagccccc ggcccggacg ggcggccctc gcggcagcac
     6001 ccccgcaaag gggctggcga gggacaaagg gctgggcgct cggcgcgagt cggtcccggc
     6061 gtcgcgcggg ccggaccgcg cgcggcgggg cgcgcgccta cacgatccgg gggagcgctc
     6121 cgcggatgct tccggagtga tagcgcaact caccggaggc aacagccgcc tcgagtgcat
     6181 cgacgtcgcc acggaacagc cgtcccgccc ggtcgaggac ggcgcggcct gcggattcgg
     6241 aaggcagcac gttgacgacg aagcgcgccg cgcccagcag ggcgtgccac gcgcgcatca
     6301 gttcgcctgg atccggcgct cctccttcga agtcgagtcc ctggaagtcc tcggcggtgt
     6361 accggctcgc ccgcgcggcc tcctcgagga tcgccgcagg gctgaacccg gggtccttcc
     6421 cgcacgccgc ccacgccagg tacccgagtg gctgcacctc ccggtcgcag ttcacgacgt
     6481 ccaggaagtc gcggggctca acccggccca cgagcgcgag caccttgctc gtcgccagat
     6541 cgaagggatg gaggacgagg cccagttcgt cgtgttccac cagcgggaag aaccggaacg
     6601 cgctgtcccg cgcccattcc atgagcacag tctcgggtcc acggcgcacc tcggcttcca
     6661 cgaagccggg ccgctcgcgg acggtgcgga cggcgtagcc ggcgcgctcc aggtccagcc
     6721 ggtccgcgtg ccacgacgcc tgcagcgcct cctcggtgtc gtggaacacg tcgatgtccc
     6781 tcgagagcct cggcgcggcg atcagttcgt tgagcgtggc tccgcccgcg aagtaactct
     6841 cgccgctccg gcgccggttc gccgctatga gccggcagac gtctcgctga tagctgctga
     6901 gacccatcgc ttgagttctc ccgcccgctg gaaggcgctg aggtcgccgt gccgctgaag
     6961 gctgtccagc acccgcaagc gttccgcgac cgtgacgggg taataaccct tctcgaggaa
     7021 ccacaggcag cgatctcggt actcgtccac cagggcccgc agcgcggctt ctcgttcctg
     7081 gtcggcggtg ctcatgcggc gatcatacgc gggttggagc cgcgcgggga gtgcgcattc
     7141 gcccgggcgc cgaggctcct cggcggccag cgcgagccga gggaagcgat cgtcgcagcg
     7201 cctgttcgat ctcgtcgtac agcccaatcc gttcgccatc ggtcagatgg acggctacca
     7261 cgtcgacggc ggcttccacc aggttcttcg tgtcgtaccc gacatcgacc cggtagacgg
     7321 cggcgacact cctggcaaac aggagggcgt tacacagcag cgccatggct agtgtcacct
     7381 gtgccaccac aatcagctga cgtgtccagt gacgggagac cggcgcagaa aaggcacttc
     7441 cctggaacgc gacgccgact ggtgcggcgg cccggctgcg gcgggtgatg gccgtcacaa
     7501 gcgcgctggc aacgaaggcg gagaccatgg cgccggcaaa cgcgcggagg ctgaagggcg
     7561 ggccgacgtg atcgaagatc gggcccgatc gcggcgcgag gacggccgcc gcccgcggca
     7621 gagcgtaggc gatgggaagc gcgagtacgg ccccggcgcc aatctcgctc gcgtcgtcga
     7681 tcccgcccgg ctcaaggcgg cgctgagagt cccggagacc caggcgaaag acgtgcaact
     7741 cgggcagcgc gcggcgattg acacccgcac gggaccgatc acggggcgcg tggcgcgggt
     7801 ggacccggcg tccaccggcg gcacggtcac ggtggacgta gtgctggacg ggacgatgcc
     7861 gaaaggcgcg cggcccgacc tgacgattga cggcacgatg acctgccgct gaagtaccgg
     7921 ggcctgacgc ccgcggaacg gcggccgctg gtggcggcgg cactcgagcg ggtggagatg
     7981 gcgcaccggg cgaagcacct gccggggcag ctctcgggcg gccagcagca acgtgtggcg
     8041 gtggcccggg cggtggtggg caaggcggcg atcctgcccc cgtccgtgcc ttgccccttg
     8101 ccccttgtcc cttgcccctg tgccgccgat actcctgtgt cgtgatccgc gtcgaccacc
     8161 tgaccaagcg ctacggcccc ctgaccgcgg tcgacggcct gtcgttctcc gt
//
LOCUS       DAOSIF010000076         8179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8174_length_8179_cov_3.612752, whole genome shotgun sequence.
ACCESSION   DAOSIF010000076 DAOSIF010000000
VERSION     DAOSIF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8174_length_8179_cov_3.612752"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLT35_04120"
     CDS             <1..537
                     /locus_tag="PLT35_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOG28443.1"
                     /translation="RGRHQVENAVVAVRLLEALDGAGVSVSGDAIEQGLRSARWPGRL
                     ELFRMADGRTALVDAAHNAAGAAALARYLGEAAPRGQPLVFAAMRDKDHAAMLGHLVP
                     HATRLVLTAPPTPRAARPEDLEAVARAVDPRARVIVEADPITALAAAWAAAPDIAVAG
                     SIYLLGAVLPWLAAEAAG"
     gene            621..2927
                     /locus_tag="PLT35_04125"
     CDS             621..2927
                     /locus_tag="PLT35_04125"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOG28444.1"
                     /translation="MRLRASTLPWLALLALLVAAEGSAQEAQPSALPGVDRSRQWRLE
                     RIGESHWKLTGDVEIEREGTLIFADEVEIFTDRDLLTARGNVTLTTENQRISADSLEF
                     NTKTRLGTFHHASGSVLLQQAHEQPAEKSEFGTQEPEVFFYGETLEKLGERKYRITRG
                     GFTTCVQPRPRWQLTSGTIILNIDHYALLVHSMMRVKGVPVLYLPVVYYPINKEDRAS
                     GFLLPVYGSSRYKGHTLSNAFFWAIGRSRDATFTHDWFSKTGHGFGAEYRYVRSRSSS
                     GEARVYLLREKPTSYVDSTGSTVGVAGSRSFDARGSLAQDLPWKLRARARVDYFSDIA
                     VQQAYNTNVYDASRRQRSYSGSVSGTWGAYSVTASADRSEFFFNTTDSTVSGSMPRLT
                     VSRGEKPFKGLPVYFGVTGEYASLLRETHHGNTGTDTGLRRLDVSPAVRVPFRKWAFF
                     TVNTSIGWRYTRWEESLDPLTGLQVPAAVDRSYVDLQTRIVGPVFNRIWNTPDNGYAE
                     KFKHTIEPWVNVQRVTAFDESLPIVRLDSADHVVGGTTRITFGVNNRLYAKRKTGPDA
                     GRSREILNVTARQTYYTKAAASTVDSSYSTSFGSAEAQKMSAVSLAARLSPSEGLNAT
                     FRGEFNPYKNAFMTLGASGTYAAGETMLLSAGWSRRRFIPDLPGYDSEASATHYLNAD
                     ATFRFSQNRFGGSLSANYDIKQASFLQRRVVAYYNAQCCGLTVSYQTYNFGGFASRAL
                     NEDRRFSISISLAGIGSFSPPFGGASPR"
     gene            2957..4003
                     /gene="rfbB"
                     /locus_tag="PLT35_04130"
     CDS             2957..4003
                     /gene="rfbB"
                     /locus_tag="PLT35_04130"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOG28445.1"
                     /translation="MTKVLVTGGAGFIGSNYVRHALRTHADWHVTTLDKLTCCGRLES
                     LHDVLDHPRHAFVLGDVADSAVSAPLVRSADLVVHFAAETHADRSIQEAGAFIHTDVF
                     GTFVLLEAARAAPGLRRFVQVSTDGVYGSIAAGASKETDELRPRTPSTASKAGADRLA
                     YSYWATHEVPVIVTRASNNYGPYQPPDKMIPLFITNAFEDREVPLYGDGLHVRDWLHV
                     EDHCRAIDAVLERGACGEAYNVGGGNEISNADLTKRILGLAGKPESLIRFVADRKGHD
                     RRYCLDTTKLRGLGWSPQRSFEAGLSATVDWYRANPWWWRPIKTRDAACPACSRAQCG
                     ERAGAQASSGPGER"
     gene            4000..4950
                     /locus_tag="PLT35_04135"
     CDS             4000..4950
                     /locus_tag="PLT35_04135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG28446.1"
                     /translation="MTGLPTVLVTGAAGFAGSHLLDLLAGDPVCVAGLRKPGVGAATQ
                     ALYPGVTWLEVDLLDRDATRRAIGELAPAVIYHLAGSPHVGQSWRAAAETLSANAMAT
                     HHVLDAVRAEGLRSRVVVPSSAYVYRPADRALTEDDAIEAANPYAISKVATELAVVKA
                     AEEDGVPAVVARAFNHTGPRQDPSFFVPGVARQVAAIEAGRAEPVIRVGNLATRRDLT
                     DVRDTVRAYRALAERGLPGRVYNVCSGRAYWMRDLLDLLVGLARVRVTVEVDAARLRP
                     NDTPLLLGDPSRIRRETGWEAGVPIERTLQDLLDFWRRAA"
     gene            4955..5905
                     /locus_tag="PLT35_04140"
     CDS             4955..5905
                     /locus_tag="PLT35_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG28447.1"
                     /translation="MRVLVTGGTGFLGRAVVRAVAEAGHEAIVFARRASASGLPGQAF
                     DGDIRDEGALLAASEGCEAVLHGAALVSTWKARSRDFDDVNVGGLQAVLRAARRRGMR
                     RIVYTSSFMALAPGGLAQPPRWNDYQRTKAAGDAIARREASRGAPLVCLYPGVVYGPG
                     PATEGNLVGGLIGDHLRGRLPGVVGGDRIWSFAYLDDVAAAHVAALGAGRPGARYHLG
                     GENAPPMRAFDIVRALTGRPLPRRIPDWLASPAALACELRASWFGRPPRLTTGTLEVL
                     LHDWPLGHARAADDLGYRVTPLDEGVARVVRALQDEGAGA"
     gene            5908..7242
                     /locus_tag="PLT35_04145"
     CDS             5908..7242
                     /locus_tag="PLT35_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOG28448.1"
                     /translation="MSPETARQLVHISMSAFALLLRVLTWWQAALCAAAALAFNVFVL
                     PRVGGSRLARPADAVRGYSLGIVLYPVAVLALILAFPARLDIAGAAWGIMALGDGAAT
                     LAGRALGRHRLPWNPDKTLEGLLGFLTAGGASGVFLSWWIAPAVAPAPAPAFTLLAPL
                     AAAAAAGLAETLPIQLDDNITVPAVAGGALWLGSLVSGEAWLANASAVAARLAPAAAV
                     NTVAAAAGWIAGTVRASGTLAGWLIGVAVYAGLGPGGWLLLFATFFAAAATSRLGLAR
                     KTLLGIAEERGGRRGAGNAFANTGLAALAALAAVTTPFRAEAAVAFVAALAAGGGDTV
                     ASEIGKAWGRTTYLPLTRSRVRPGTPGAVSIEGTAAGLVGALALAALGAWLGLVPWWT
                     IWAVAAGAAAGSAVESLLAATLEASRTLNNDALNFLNTATAASVSVAITRLL"
     gene            7239..8156
                     /locus_tag="PLT35_04150"
     CDS             7239..8156
                     /locus_tag="PLT35_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOG28449.1"
                     /translation="MNAPSRLRTIADFARPYTLIAPSLGFASAAATAIGAYPREPWSW
                     GLLAAPAVGTIMAAVLNVASNGLNQIFDLDIDRINKPRRPLPSGRLSLGAAWAVTLAG
                     FAAALGLAWFVAPGGRHECFWMVLAAALMTVAYSAPPLRTKRLGVWANVTICIPRGLL
                     LKVAGWSSVKTALGLEPWYLGSIFALFLLGATTTKDFADMDGDRRGGCRTLPLQYGVA
                     KAAWLIAPSFVIPFALIPAGAWAGLLTGSFWLLQLLGLVMIAYGAYVCFLMLRRPDSL
                     AVEENHVSWAHMYRMMFVAQIGFALAYLL"
BASE COUNT         1054 a         2964 c         2964 g         1197 t
ORIGIN      
        1 cgcgggcgcc accaggtgga gaatgcggtc gtggccgtgc gcctgctcga ggcgctggac
       61 ggggcgggcg tctccgtgtc tggcgacgcc atcgagcagg gcctgcggag cgcacggtgg
      121 ccagggcggc tggagctgtt ccggatggcg gacggccgga cggcgctcgt ggacgcggcg
      181 cacaacgccg caggcgcggc ggcgttggcg cgctacctcg gcgaggcggc gcctcgaggg
      241 cagccgctgg tcttcgcggc gatgcgcgac aaggaccacg cggccatgct cggccacctc
      301 gtgccgcacg ccacgcgcct ggtgctgacg gctccgccca cgcctcgggc cgcgcggccc
      361 gaggatctcg aggccgtggc gcgggcggtc gatccccgcg cgcgggtcat cgtcgaagcc
      421 gacccgatca cggccctggc ggccgcatgg gctgccgccc ccgacatcgc cgtcgcggga
      481 tcgatctacc tcctgggcgc ggtgcttccg tggctggccg ccgaggcggc cggctgaggc
      541 cacggatccc gtgtggcggc gggcgcggcg gccgcgccgc ggttggcccc ctctgtcgcg
      601 cgtggtaatc ttcggcgcag atgaggctgc gcgcgtcgac gctcccgtgg ctggcgctgc
      661 tggccctgct cgtcgcggcc gaggggtccg cgcaggaggc ccagccaagc gcgctcccgg
      721 gcgtcgatcg atcgcgccag tggcgcctcg agcggatcgg cgaatcgcac tggaagctga
      781 ccggcgacgt tgaaatcgag cgcgagggca cgctgatctt cgccgacgag gtggagatct
      841 tcacggaccg cgacctcctg acggcgcgcg gcaacgtcac gctcacgacg gagaaccagc
      901 gcatctcggc cgactcgctc gagttcaaca cgaagaccag actcggcacc tttcatcacg
      961 cgtccggcag cgtgctgctc cagcaggccc acgagcagcc ggccgagaag agcgagttcg
     1021 gcacgcagga gccggaggtc ttcttctacg gcgagacgct ggagaagctc ggcgagcgca
     1081 agtaccggat cacgaggggg gggttcacga cgtgcgtcca gccgcggccg cgctggcagc
     1141 tcacgtcggg cacgatcatc ctgaacatcg atcactacgc gctgctcgtc cactcgatga
     1201 tgcgggtcaa gggcgtgccg gtgctctacc tcccggtcgt gtactacccg atcaacaagg
     1261 aggatcgcgc cagcggcttc ctcctccccg tgtacggctc gtcgcggtac aagggccaca
     1321 cgctgagcaa cgcgttcttc tgggccatcg gccggagccg ggacgccacc ttcacgcacg
     1381 actggttctc gaagacgggg cacggattcg gcgcggagta ccgctacgtg cgctcgcgct
     1441 cgtcgtcggg cgaggcgcgc gtctacctgc tgcgcgagaa gccgacgagc tacgtggaca
     1501 gcaccggcag taccgttggc gtggcgggct cgcgcagctt cgacgccagg ggcagcctcg
     1561 cccaggacct gccgtggaag ctccgggcgc gcgcccgagt cgactacttc tccgacatcg
     1621 cggtccagca ggcgtacaac acgaacgtgt acgacgcgtc gcggcggcag cggagctaca
     1681 gcggaagcgt ctccggcacg tggggggcgt acagcgtcac cgcgtcggcc gatcgcagcg
     1741 agttcttctt caacaccacc gattcgaccg tgtccgggtc gatgccccgc ctgacggtga
     1801 gccggggcga gaaaccgttc aaggggctgc cggtctactt cggcgtgacg ggcgagtacg
     1861 ccagcctgct gcgggagacc catcacggga acaccgggac ggacaccggg ctgcggcgcc
     1921 tcgacgtctc gccggccgtc cgggtcccgt tccggaagtg ggcgttcttc accgtgaaca
     1981 cctcgatcgg atggcgctac acccggtggg aggagagcct ggacccgctg acggggctcc
     2041 aggtgccggc ggcggtggac cgatcgtacg tcgatctgca gacccggatc gtgggccccg
     2101 tgttcaaccg gatctggaac acgccggaca acggctacgc cgagaagttc aagcatacga
     2161 tcgagccgtg ggtgaacgtg cagcgggtca cggccttcga cgagtcgctg ccgatcgtcc
     2221 ggctcgactc ggccgaccac gtggtgggcg gcacgacgcg gattaccttc ggcgtgaaca
     2281 accgcctgta cgcgaaacgg aagaccgggc ccgacgcggg gaggtcgcgg gagatcctga
     2341 acgtcaccgc caggcagacg tactacacga aggcggcggc ctccacggtg gactcgagct
     2401 actcgaccag cttcggcagc gcggaggccc agaagatgtc ggccgtctcg ctggccgcgc
     2461 gcctctcccc gtccgagggc ctgaacgcga cattccgcgg cgagttcaac ccctacaaga
     2521 acgcgttcat gaccctgggc gcgtccggca cctacgccgc cggcgagacg atgctgctgt
     2581 cggccggctg gagcaggcgc cggttcatcc ctgatctgcc gggctacgac tccgaggcct
     2641 cggcgacgca ctacctgaac gcggacgcca cgttccgctt cagccagaac cggttcggcg
     2701 gctcgctgtc ggcgaactac gacatcaagc aggcctcgtt cctgcagcgg cgggtggtcg
     2761 cctactacaa cgcccagtgc tgcggcctga cggtgtcgta ccagacgtac aatttcggcg
     2821 gcttcgcgtc ccgcgcgctc aacgaggacc gccgcttcag catctccatc tccctcgccg
     2881 gcatcgggtc cttctccccg cccttcggcg gcgcgagccc ccggtaggcg cggcgctggc
     2941 ggaaggacgg actggcatga cgaaggtgct cgtgacgggc ggcgcgggct tcatcggcag
     3001 caactacgtg cgccacgccc tgcgaacgca cgccgactgg cacgtcacca cccttgacaa
     3061 gctgacctgc tgcgggcggc tggagagcct gcacgacgtg ctcgaccatc cccgtcacgc
     3121 cttcgtgctc ggggacgtcg ccgacagcgc ggtgagcgcc ccgctggtgc ggagcgcgga
     3181 cctcgtcgtc cacttcgccg ccgagaccca cgcggatcgg tcgatccagg aagcgggcgc
     3241 gttcatccac accgatgtct tcgggacctt cgtcctgctg gaggccgccc gcgcggcgcc
     3301 cggcctgcgg cgcttcgtcc aggtctcgac cgacggggtc tacggatcga tcgcggccgg
     3361 cgcgtcgaag gagaccgacg agttgcgccc ccgcaccccc tccaccgcca gcaaggccgg
     3421 ggccgatcga ctcgcgtaca gctactgggc cacccacgag gtgccggtca tcgtcacgcg
     3481 cgcgtcgaac aactatggtc cgtaccagcc tccggacaag atgatcccgc tcttcatcac
     3541 gaacgccttc gaggaccgcg aggtgcccct ttacggcgac ggcctgcacg tgcgcgactg
     3601 gctgcacgtc gaggaccact gccgcgccat cgacgccgtg ctcgagcgcg gggcgtgcgg
     3661 cgaggcctac aacgtgggcg gcggcaacga gatctcgaac gccgacctga cgaagcgcat
     3721 tctcggcctt gccggcaagc ccgagtcgct catccgcttc gtcgcggatc ggaaggggca
     3781 cgaccgccgg tactgcctgg acacgaccaa gctgcgcggg ctgggctggt cgccgcagcg
     3841 ctcgttcgag gcggggctct ccgcgacggt ggactggtac cgcgccaacc cgtggtggtg
     3901 gcggccgatc aagacgcggg acgcggcgtg cccggcgtgc tcccgcgccc agtgcggcga
     3961 acgggccggg gcgcaggcgt ccagcgggcc gggcgaacga tgaccggact gccgaccgtt
     4021 ctcgtcacgg gcgccgcggg cttcgcgggg tcccacctgc tggatctcct cgccggcgat
     4081 cccgtgtgcg tggcggggct ccgcaagccc ggcgtcggcg cggcgacgca ggcgttgtac
     4141 ccgggcgtga cctggctcga ggtcgacctc ctcgaccgcg acgccacgcg ccgcgccatc
     4201 ggcgagctcg ctccggccgt gatctaccac ctggcgggct cgccgcacgt cgggcagtcg
     4261 tggcgcgccg ccgccgagac gctgtcggcc aacgcgatgg ccacgcatca cgtgctcgac
     4321 gccgtgcgcg ccgaaggcct gcgcagccgg gtggtcgtgc cgagctcggc gtacgtctac
     4381 cgccctgccg atcgcgcgct gacggaggac gacgcgatcg aggcggcaaa cccgtacgcc
     4441 atcagcaagg ttgcgacgga gctggccgtg gtgaaggccg ccgaggagga tggagtcccg
     4501 gccgtcgtgg cgcgcgcgtt caaccacacc ggtccgaggc aggacccgtc gttcttcgtc
     4561 cccggggtgg cgcggcaagt cgcggcgatc gaggcgggcc gggccgagcc ggtgatccgc
     4621 gtcggcaatc tggccacccg gcgcgatctc accgacgtgc gcgacacggt gcgcgcctac
     4681 cgcgccctcg cggagcgcgg cctgccgggg cgcgtctaca acgtgtgctc cggccgcgcg
     4741 tactggatgc gggatctgct ggacctgctt gtcggcctcg cgcgcgtccg cgtcacggtc
     4801 gaagtcgacg cggcgcgact ccggccgaac gacacgccgc ttctgctggg cgacccctcc
     4861 cgcatccgcc gggaaacagg ctgggaggcg ggtgtgccca tcgagcggac gctgcaggac
     4921 ctgctggact tctggcggcg ggccgcgtga cggcatgagg gtcctcgtca cgggaggcac
     4981 gggctttctc gggcgggccg tcgtccgcgc cgtggcggag gcggggcatg aagcgatcgt
     5041 gttcgcgcgc cgggcctcgg cgtcgggcct gccgggccag gcgttcgacg gcgatatccg
     5101 cgacgagggc gcgctgcttg cggcgtcgga gggctgcgag gccgtgctgc acggagcggc
     5161 cctcgtgtcg acgtggaagg cgcgcagccg cgacttcgac gacgtcaacg tgggcggcct
     5221 gcaggccgtg ctccgagccg cccgccgccg cggcatgcgc cgcatcgttt acacgtcgtc
     5281 gttcatggcg ctggcgccgg gcggcctcgc ccagccgccg cgctggaatg actaccagcg
     5341 gaccaaggcg gcgggcgacg ccatcgccag gcgcgaggcc tcgcgcggcg cgccgctcgt
     5401 ctgtctttac ccgggcgtcg tctacggtcc cggccccgcc accgaaggca acctcgtcgg
     5461 cggcctgatc ggcgaccatc tgcgcggccg gcttccgggc gtggtcggcg gcgatcggat
     5521 ctggtcgttc gcgtacctcg acgatgtcgc ggcggcccac gtggcggccc tcggcgccgg
     5581 gcgtcccggc gcgcgctatc acctcggcgg cgagaacgcg ccgcccatgc gcgccttcga
     5641 catcgtgcgc gcgctgaccg ggcggccgct gccccgccgg attccggact ggctcgcttc
     5701 gccggccgcg ctggcctgcg aactgcgcgc gtcgtggttc ggccgtcctc cgcggctgac
     5761 gacgggaacg ctcgaggtgc tgctgcacga ctggccccta ggccacgcgc gggccgcgga
     5821 cgacctcgga taccgggtga cgccgctcga cgagggcgtg gcccgcgtgg tgcgggccct
     5881 ccaggacgag ggagcgggcg cctgatcatg tctccagaaa ccgcgcgcca gctcgtgcac
     5941 atctccatgt cggccttcgc gctcctgctg cgcgtgctga cgtggtggca ggccgcgctg
     6001 tgcgccgccg cggccctggc gttcaacgtc ttcgtgctgc cgagggtcgg cgggagcagg
     6061 ctcgcccggc ccgccgacgc cgtccgcggc tactcgctcg gcatcgtgct ctaccccgtg
     6121 gcggtgctgg cgctcatcct cgcctttccg gcgcgcctcg acatcgccgg cgccgcctgg
     6181 ggcatcatgg cgttgggcga cggcgcggcg acgctggccg gacgggcgct gggccgccac
     6241 cggctcccct ggaaccccga caagacgctc gaggggctgc tgggcttcct cacggccggc
     6301 ggcgcctccg gcgtgttcct gtcctggtgg atcgcgccgg cggttgcgcc ggcgccggcg
     6361 cccgccttca cgctgctggc gccgcttgcc gcggccgccg ccgccgggct ggccgagacg
     6421 ctcccgatcc agctcgacga caacatcacg gtaccggccg tggcgggcgg cgccctgtgg
     6481 ctgggcagcc tggtcagcgg cgaggcctgg ctggccaacg ccagcgccgt cgcggcccgt
     6541 ctggcgccgg cagcggcggt gaacacggtg gcggccgcgg cgggctggat cgccggcacg
     6601 gtgcgcgcat ccggcaccct cgccggctgg ctcatcgggg tcgcggtcta cgccgggctg
     6661 gggccggggg gctggctgct gctgttcgcg acgttcttcg ccgcagctgc gacgtcgagg
     6721 ctcggcctgg cgcgcaagac gctgctcggg atcgccgagg agcgcggcgg ccgccggggc
     6781 gcgggcaatg ccttcgccaa cacgggcctg gccgccctcg cggcgctcgc ggccgtcacc
     6841 accccgttcc gcgcggaagc cgccgtggcg ttcgtcgccg ccctggcggc cggcggcgga
     6901 gacacggtgg cgagcgagat tggcaaggcc tggggccgga ccacgtatct gccgctcacg
     6961 cggtcgcgcg tgcggcccgg cacgcccggc gccgtgtcga tcgagggcac ggcggccggc
     7021 ctcgtcgggg cgctcgcgtt ggctgccctc ggcgcgtggc tcggcctcgt gccgtggtgg
     7081 acgatctggg cggtcgcggc gggggcggcg gcggggtcgg cggtcgagag cctgctcgcc
     7141 gccacgctgg aagcgtcccg gacgctcaac aacgacgccc tgaacttcct caacacggcg
     7201 acagccgctt ccgtatccgt cgcgatcacg aggctcctgt gaacgcaccc tcgcggctcc
     7261 gcacgattgc cgacttcgcc cgtccgtaca cgctcattgc cccgtcgctc gggttcgcct
     7321 ctgccgccgc cacggccatc ggggcctatc cgcgggagcc ctggagctgg gggctcctgg
     7381 ccgcgccggc cgtcgggacc atcatggccg cggtgttgaa cgtcgcctcc aacggcctga
     7441 accagatctt cgacctggac atcgaccgca tcaacaagcc caggcgtccg ctgccgagcg
     7501 ggcggctgtc gctcggcgcg gcgtgggcgg tcacgctcgc cggcttcgcc gccgcgctcg
     7561 gcctcgcctg gttcgtcgcg ccgggcggcc ggcacgaatg cttctggatg gtgctggcgg
     7621 ccgcgctgat gaccgtcgcc tactccgcgc ctccgctgcg gaccaagcgc ctcggcgtgt
     7681 gggcgaacgt cacgatctgc atcccgaggg ggctcctgct gaaggtcgcc ggctggtcct
     7741 cggtgaagac cgcgctgggc ctggagccgt ggtacctcgg gtccatcttc gcgctcttcc
     7801 tgctcggcgc gacgaccacc aaggacttcg ccgacatgga cggcgatcgc cggggcggat
     7861 gccgcacgct gcccctgcag tacggcgtcg cgaaggcggc ctggctgatc gcgccgtcct
     7921 tcgtgatccc gttcgcgctc atccccgccg gcgcgtgggc cggtctcctg accgggagct
     7981 tctggctcct gcagctgctc ggcctcgtga tgattgccta cggcgcctac gtctgtttcc
     8041 tgatgctgcg ccggcccgac agcctcgccg tcgaggagaa ccacgtctcg tgggcgcaca
     8101 tgtaccgcat gatgttcgtc gcccagatcg ggttcgcgct ggcctatctg ctttgaccag
     8161 ccgagggcgc gccgaggcg
//
LOCUS       DAOSIF010000077         8114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8255_length_8114_cov_4.236506, whole genome shotgun sequence.
ACCESSION   DAOSIF010000077 DAOSIF010000000
VERSION     DAOSIF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8255_length_8114_cov_4.236506"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLT35_04155"
     CDS             complement(<1..506)
                     /locus_tag="PLT35_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28450.1"
                     /translation="MRHGTSNARGLSLVEVTIMLLVLMLLTGVLAPSIMDYVRDAQWV
                     KVKEDCEAIGVSVARLARDVGPCLKFDGTAPCTKANRVDILYSDGPDVTSADLAGDAV
                     WDFHGGGATASDLNWDRDDSRGDSMEHQFVDNGTGPMYPTPSDLGSYGTSGPAFGLGW
                     RGAYLSPP"
     gene            complement(850..1977)
                     /locus_tag="PLT35_04160"
     CDS             complement(850..1977)
                     /locus_tag="PLT35_04160"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG28451.1"
                     /translation="MLDALRHHKPAPPASPDQPPGRAAGPDAVPASLAFQAERERQED
                     HSLARRRLGLVIVLVAATAALVWQAAAMSGLITSGPFTSFRKAPAAATRAAPPPLPAR
                     PAAGPPQRAEPAAVPEAPPVSAEPPARPVEERSSPPAGGAAKPPGGKAGARAPRVEGA
                     APAAFPPPAPGAGPGAPAISRAPAAETEHFKRALYYQRVGDFENALIHYRAVLAADEL
                     NAEAHNNLGVLYQSKGLLEEAIGEFQRATFIDPRYEKAQNNLGVALMRSGRTDAAVVQ
                     FRSVVARDPKNVEALTNLSLALKAAGRVEEARESLQRVLALNGRYAQAHYNLALIYEE
                     RGELQRAIDHYEQFLANAGPESAALSVDVRARVEALRTGLR"
     gene            complement(1990..2913)
                     /locus_tag="PLT35_04165"
     CDS             complement(1990..2913)
                     /locus_tag="PLT35_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG28452.1"
                     /translation="MYEDYFGFTEKPFSLTPDPKYLYRSESHANAFELLQYAIRRREG
                     FVVVTGDIGTGKTTLCRALLEQIDRRTFSALVLNPFLSEEDLLKLILQDFGVVSREEM
                     KTGRLQGVSKQELIDTLYDFLLGLLPIHAGAVLIIDEAQNLPLQVLEQLRILSNLETD
                     KEKLLQIIMVGQLNLQPLLRSPGLRQLDQRVSIRYELKPLTRDEMVAYIAHRIQVAGG
                     STAVAFTPKALQQVHHYSGGIPRLVNLVCDRALLGAYSARASKVTHDLIERAAETLDL
                     RPIRRARFAWLRRGAAVTDAARSHPSSLSCR"
     gene            complement(2953..4533)
                     /locus_tag="PLT35_04170"
     CDS             complement(2953..4533)
                     /locus_tag="PLT35_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HOG28453.1"
                     /translation="MLLVAAAVLAAPGLAPAQSPYPTAPATTGSFPPASAPNPQGPGL
                     PPLAVSQIEEGRRSDAMAQTLSLSFAEPVPIRDLLLLLVRDTDLSVVVDPDAQGTFTG
                     ELKNVTLRQALDTILEPHGLHAVITDNVVRVGRERAETRLFNINYVATRRSGTRGSGL
                     AGGLAGNAGLGAYGGAGAIYAGGVGAAGYGGGYYAGTGGIGGSQASVSGTDSGDVFEE
                     IQAGVQTLLSDAGKMNLDRKAGLLQVTDVPSRLDKIAVYIEAYENRALRQVRIVAKIL
                     EVELSEGYSAGIDWSLVLKRADKAATLTQNLAPTSGSGAFTLGLNIKDFAGLLDAFAS
                     QGRVNVMASPWVTAMNNEPAVMRVGTQDVFFVTTSQVDASTGQILQTVVTPQTITEGV
                     VLSVTPQIAAGGIINLSIMPSITERTGTATSRLGDQVPVVSVRETDTLVRVHQGETIV
                     IAGLMQDRTTVEKSKVPVLGDVPVVGGLFRRDERTKRKTDLVILLTPTVMTPGEMAAA
                     AAADQQRLYEAQRAPVRK"
     gene            complement(4811..6319)
                     /locus_tag="PLT35_04175"
     CDS             complement(4811..6319)
                     /locus_tag="PLT35_04175"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOG28454.1"
                     /translation="MRKLAFLPGALMVAAGVVWAALALRWTTPAAVLAAGGAAALAVG
                     VAASWRDVREWLGDPRGVFALNAALSTLLLAAVLVLVNALAARRAPAFDWTEAGRHTL
                     AAETVRILEDLGEDVVFTQWGRTRDPGVGRLLESFASRSPRLAVRFADLDASPQAARE
                     HGVTRAGTVVVSSPRRFRKVERVTEPALATAILQVTSPAEPAVCFASGDGEHGLEDTG
                     PQGLSTLASVLAASNFAPRRVSLLEGDAAQGCAALVVPGVPRAFPAGALERVSAHLAR
                     GGRVLLALDPPVDPGVAGFLRRLGIVAGQGVIIETSGAGRAVGAGPENPVSFAYHDHP
                     VTRGLDQRAIFGRAAPLAVAPTEIGRPIALVSTADSAFERADLESQFTGFQAGRDRRG
                     PFALAVATSIGRGARDAALPEPRLVVTGDSDFLANGLIGWSANRELAVRIVSWLAGVD
                     EARIVSVEERQNRRVALTERRRAWMYIVNLGVLPLLPLAARLLHLLRTRSRA"
     gene            complement(6316..7101)
                     /locus_tag="PLT35_04180"
     CDS             complement(6316..7101)
                     /locus_tag="PLT35_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG28455.1"
                     /translation="MRHALAIARRELESYFVSPIAAAVMAVFTFVSGLFFSNMLVHYI
                     RQAALADSQIQRLGRSDYQLDVPTTVLAQFFQNEAFLLMLAVPLLTMGLMTDERRKGT
                     IELLMTSPVRASEIVAGKFLGAIALLALMLAPLVPMFAFMGAGNHWEAGVIASGLMGL
                     LLVGGAQIALGLFVSSLCEHVLVSAIGTYGIVVTLQFIDTTGGGSGSVWARMLGAFSF
                     YARQIDSTRGVVSVADLVYFASWIVMALFLAQRAVEALRFERT"
     gene            complement(7101..>8114)
                     /locus_tag="PLT35_04185"
     CDS             complement(7101..>8114)
                     /locus_tag="PLT35_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28456.1"
                     /translation="EVLGLLGPNGAGKTTTLRMITGSVPPSRGRVRIGGFDVFERPID
                     AKRLLGYLPEQPPVHPEMTVRRYLRFVAALKDVPAARTAAAVDRAIGRARLGDAADAA
                     IRTLSKGYRQRVGLAQAIVHEPPVLVLDEPTSSLDPKQRAEVRDFILELKGRHTVVLS
                     THILPEVAGVADRIVILHRGRVMAEDTPERLGERLRARDAVRVVVGGAGTAAGAAGVP
                     APMPEDVTLALAALPGAIRVSIITGADSAGAIEARVESGRGTDLRAEAAALVVSRGWR
                     LLGLAGESLSLEDVFLALTDGSGREGGGESKGVGDGSGSGRGGASAEAGGGGGTAGGR
                     GGA"
BASE COUNT         1092 a         2987 c         2922 g         1113 t
ORIGIN      
        1 atgggcggcg agaggtaggc gccgcgccag ccgaggccga aggccgggcc ggacgtgccg
       61 tagctcccca ggtcgctcgg cgtcgggtac atcgggcccg tgccgttgtc cacgaactgg
      121 tgctccatcg agtcgccgcg gctgtcgtcg cggtcccagt tgaggtcgga ggccgtggcg
      181 ccgccgccgt ggaagtccca gaccgcgtcg ccggccaggt ccgcgctcgt cacgtccggg
      241 ccgtcgctgt agaggatgtc gacgcggttc gccttcgtgc acggcgccgt gccgtcgaac
      301 ttgaggcacg ggccgacgtc gcgcgcgagg cgcgccacgc tgacgccgat ggcctcgcag
      361 tcctccttca ccttcaccca ctgcgcgtcg cgcacgtagt ccatgatcga cggcgccagc
      421 acgccggtca ggagcatcag gacgagcagc atgatggtca cttccaccag tgacaggccg
      481 cgtgcgttcg aagttccgtg tcgcatgtga tcctcgtccc gcacctcggc cggcggggcc
      541 tgccgcccgt tcccgggcca cccgccgtgt cgttcccgcg cgggtacgct ccggtacgga
      601 ggtgcgacga aggacgctcg agcaaggcac gtgccccggc ccggccgcgc ggcgcccccg
      661 gcgcaggatg gcgcgacgtc gcgtctgccc aacgacttcc ggcgaactgg cgcgccgcgc
      721 cggggctgcg cgcggctgcg cggacctgca cttcgcgatg agtgtgcggg aagcgcgcta
      781 caacgggcga ggggcggccg gcggcccggg cggcggcggc ggggccggga gggcgagcga
      841 gggagccgcc tacctgagcc ccgtccggag cgcctcgacg cgcgcccgga cgtccaccga
      901 cagcgccgcg ctctccggcc cggcgttggc gaggaactgc tcgtagtggt cgatggcgcg
      961 ctgcagctcg ccgcgctctt cgtagatcag ggccaggttg tagtgggcct gcgcgtagcg
     1021 gccgttgagg gcgagcacgc gctggagcga ttcgcgggct tcctcgacgc ggcccgcggc
     1081 cttcagcgcg agcgagaggt tcgtcagcgc ctcgacgttc ttcgggtcgc gcgcgaccac
     1141 cgaccggaac tgcacgacgg ccgcgtcggt cctgccggag cgcatgaggg cgacgccgag
     1201 gttgttctgg gccttctcgt agcgcgggtc gatgaaagtc gcgcgctgga actcgccgat
     1261 ggcctcctcg agcagcccct tgctctggta gagcacgccg aggttgttgt gggcctcggc
     1321 attcagctcg tcggccgcga ggacggcgcg gtagtgaatg agggcgttct cgaagtcgcc
     1381 gacgcgctgg tagtagagcg cccgcttgaa gtgctccgtc tcggccgccg gggcccgcga
     1441 gatggcgggc gcgcccgggc ccgcgccggg cgccggcggc gggaacgcgg ccggcgcggc
     1501 gccctcgacg cgcggcgccc gcgctcccgc cttcccgccc gggggcttcg cggcgcctcc
     1561 ggcgggcggg ctcgatctct cttcaaccgg cctggccggg ggctcggccg acaccggcgg
     1621 cgcctccggc acggcggccg gctcggctct ctgcggcggc ccggcggccg gccgcgccgg
     1681 cagcggtggc ggcgcagccc gtgtcgcggc cgccggggcc ttgcgaaagg acgtgaacgg
     1741 cccggacgtg atcaggccgc tcatcgccgc cgcctgccag accagcgccg cggtcgccgc
     1801 gaccagcacg atcacgagcc ccagacgcct gcgcgccagc gaatggtcct cctgcctctc
     1861 ccgctcggcc tggaacgcga gcgaggccgg cacggcgtcc ggcccggccg cgcggccggg
     1921 cggctggtca gggctggccg gaggcgctgg cttgtggtgg cgcagcgcgt cgagcatgcg
     1981 gctcacgctt cacctgcagg acagagacga cgggtgcgag cgggcggcat ccgtgacggc
     2041 ggcgccgcgc cgcagccacg cgaaccgcgc tcgccggatc ggcctcaggt cgagcgtctc
     2101 ggcggcgcgc tcgatgagat cgtgcgtcac cttgctcgcg cgggccgagt acgcgccgag
     2161 cagcgcgcgg tcgcagacga ggttgaccag gcgcgggatg ccgccgctgt agtggtgcac
     2221 ctgctgcagc gccttcggcg tgaacgcgac ggcggtcgac ccgcccgcca cctggatgcg
     2281 gtgcgcgatg taggccacca tctcgtcgcg cgtcagcggc ttcagctcgt accggatcga
     2341 cacgcgctgg tccagctgcc gcagcccggg cgaccgcagc agcggctgca ggttcagctg
     2401 ccccaccatg atgatctgca gcagcttctc cttgtccgtc tcgaggttcg acaggatccg
     2461 cagctgctcg agcacctgga gcggcaggtt ctgcgcttcg tcgatgatga gcaccgcccc
     2521 ggcgtggatc ggcaagaggc ccagcaggaa gtcgtagagc gtgtcgatca gctcctgctt
     2581 gctcacgccc tgcaggcggc ccgtcttcat ctcttcccgc gacaccacgc cgaagtcctg
     2641 caggatgagc ttgagcaggt cctcttccga caggaacggg ttcagcacca gcgcggagaa
     2701 cgtgcggcgg tcgatctgct cgagcagcgc gcggcacagc gtggtcttcc ccgtcccgat
     2761 gtccccggtg acgacgacga agccctcgcg ccgccggatg gcgtactgca gcagctcgaa
     2821 ggcgttcgcg tgcgactcgc tccggtacag gtatttcggg tccggagtca ggctgaacgg
     2881 cttctccgtg aacccgaagt agtcctcata catcgcccgt ctccagggtg gaccgccggg
     2941 cggtcccgcc ggtcacttgc gcaccggggc gcgctgcgcc tcgtacagcc gctgctggtc
     3001 cgccgccgcc gccgcggcca tctcgccggg ggtcatcacc gtcggcgtca gcaggatgac
     3061 caggtccgtc ttccgcttcg tccgctcgtc gcggcggaac aggccgccga cgacgggcac
     3121 gtcgccgagg acgggcacct tgctcttttc gaccgttgtc cggtcctgca tcaggcccgc
     3181 gatgacgatc gtctcgccct ggtggacgcg gacgagggtg tccgtctcgc gcacgctcac
     3241 gacgggcacc tggtcgccca ggcgcgaggt ggccgtgccc gtgcgctcgg tgatgctcgg
     3301 catgatgctc aggttgatga tgccgccggc cgcgatctgc ggcgtcacgc tcagcaccac
     3361 gccctcggtg atcgtctgcg gcgtcaccac ggtctgcagg atctgccccg tcgacgcgtc
     3421 cacctgcgac gtcgtcacga agaacacgtc ctgcgtgccg acgcgcatga cggccggctc
     3481 gttgttcatg gccgtcaccc acgggctcgc catcacgttc acgcggccct ggctcgcgaa
     3541 ggcgtccagc agcccggcga agtccttgat gttcaggccg agggtgaagg cgcccgagcc
     3601 gctcgtgggc gcgaggttct gcgtcagcgt ggccgctttg tccgcgcgct tgagcacgag
     3661 cgaccagtcg atgccggccg agtagccttc cgacagctcc acttcgagga tcttcgcgac
     3721 gatgcggacc tggcggaggg cgcggttctc gtaggcctcg atgtagaccg cgatcttgtc
     3781 cagccggctg ggaacgtcgg tgacctgcag caggcccgcc ttgcggtcga ggttcatctt
     3841 gccggcgtcc gacaggagcg tctggacgcc cgcctggatt tcctcgaaga cgtcgcccga
     3901 gtccgtgcct gacaccgagg cctggctgcc gccgatcccg cccgtgcccg cgtaatagcc
     3961 gccgccgtag ccggccgcgc cgacgccgcc cgcgtagatg gcgccggcgc cgccgtaggc
     4021 gcccaggccc gcgttgcccg ccaggccgcc ggcgagcccc gagccgcgcg tccccgaccg
     4081 gcgcgtcgcg acgtagttga tgttgaagag gcgcgtctcg gcccgctcgc gcccgacacg
     4141 cacgacgttg tcggtgatga cggcgtgcag gccgtgcggc tcgaggatgg tgtcgagggc
     4201 ctgccgcagc gtgacgttct tgagttcgcc ggtgaaggtg ccctgcgcgt ccggatcgac
     4261 gacgacgctg aggtccgtgt cgcggacgag cagcaggagg aggtcgcgga tcgggaccgg
     4321 ctcggcgaag ctcagcgaga gcgtctgggc catcgcgtcc gaccggcggc cctcctcgat
     4381 ctggctcacc gccagcgggg gcagcccggg gccctgggga ttcggggccg acgccggcgg
     4441 aaacgagcct gtcgtggccg gggcggttgg atacgggctc tgcgccggag cgaggcccgg
     4501 cgccgccagg acggccgccg cgaccagcag caccggggtc agatccgcat atttgtagat
     4561 ctgaccccga ttgcggccgg gacgggcgtg tgcactgcgt gtcatgtcgt accccgggcg
     4621 gccggcggag acggatctcc gcggaggctg ctcgccttct cctcgggttt ttcggtgtat
     4681 ctcttttggg acgttagggc tgcgggcgct tctgcgcgcc attgcacagt cggggtctcg
     4741 ccccgacttg cggcacgcgg ccgggcttct ggagccgcaa atcgaggcct ggcacgatcc
     4801 ctgactccac tcaggcgcgc gagcgggtgc ggaggaggtg cagcagccgc gccgcgagcg
     4861 gcaggagcgg cagcacgccc aggttgacga tgtacatcca ggcgcggcgg cgttcggtca
     4921 gcgcgacccg ccggttctgg cgctcctcga cgctgacgat tcgcgcctcg tccacgcccg
     4981 cgagccacga cacgatccgg accgccagtt cgcggttggc cgaccagccg atcagcccgt
     5041 tcgccaggaa gtccgagtcg ccggtcacga ccaggcgcgg ctcgggcagc gcggcgtcgc
     5101 gcgcgcctcg gccgatgctc gtcgccacgg ccagcgcgaa cggccctcgc cggtcgcggc
     5161 cggcctggaa cccggtgaac tggctctcga gatcggcgcg ctcgaacgcg ctgtcggccg
     5221 tcgagacgag cgcaatgggc cgcccgatct ccgtcggcgc cacggccagc ggcgccgcgc
     5281 ggccgaagat cgcccgctgg tcgaggccgc gcgtgacggg atggtcgtga taggcgaacg
     5341 acaccgggtt ctccggcccg gcgccgaccg cgcgcccggc tccgctcgtc tcgatgatga
     5401 cgccctgtcc ggcgacgatc ccgagccgcc gcaggaaccc ggccacgccg ggatcaacgg
     5461 gcggatccag cgccaggagc acgcgccctc cccttgccag atgcgcgctc acccgctcga
     5521 gcgcgccggc cgggaatgct cgcggcacgc ccgggaccac gagggccgca cacccctgcg
     5581 cagcgtcgcc ttccagcagg ctgacgcgcc tgggcgcgaa gttcgatgcg gcgagcaccg
     5641 acgccagcgt cgacaggccc tgcggcccgg tgtcctccag gccgtgctcg ccgtctccgc
     5701 tcgcgaagca cacggccggc tcggccgggc tggtcacctg caggatggcc gtcgccagcg
     5761 ccggctcggt cacgcgctcg accttcctga agcgccgcgg cgacgacacg acgaccgtcc
     5821 cggccctggt gacgccgtgc tcccgcgcgg cctgcggcga cgcgtcgaga tcggcgaacc
     5881 ggacggccag ccgcgggctt ctcgacgcaa acgactcgag gagccgccca acgccggggt
     5941 ctcgcgtgcg gccccactgc gtgaagacga cgtcctcgcc gaggtcctcg aggatgcgga
     6001 ccgtctccgc cgccagcgta tggcgtccgg cctcggtcca gtcgaaggcc ggcgcgcgcc
     6061 gggccgcgag cgcgttgacc agcaccagca ccgcggccag cagcagcgtg gacagcgccg
     6121 cgttcagcgc gaacacgccg cgcgggtcgc cgagccactc gcgcacgtcg cgccacgacg
     6181 cggccacgcc gacggcgagg gcggcggcgc cgcccgccgc taacaccgct gcgggcgtgg
     6241 tccagcgcag cgcgagcgcg gcccacacca cgccggccgc caccatcagc gcgccgggca
     6301 ggaaggcgag cttcctcacg tccgctcgaa cctcagggcc tcgaccgcgc gctgggcgag
     6361 gaagagcgcc atcacgatcc acgacgcgaa gtagaccagg tcggccaccg acaccacgcc
     6421 ccgcgtcgag tcgatctggc gggcgtagaa cgagaacgcg ccgagcatcc gcgcccacac
     6481 cgaaccggac ccgccgccgg tcgtgtcgat gaactgcagc gtgacgacaa tcccgtacgt
     6541 gccgatggcg gacacgagca cgtgctcgca gagcgacgac acgaacaggc ccagggcgat
     6601 ctgcgccccg ccaacgagca gcaggcccat gaggccgctc gcgatcacgc cggcctccca
     6661 gtggttgccg gcgcccatga acgcgaacat cgggacgagc ggcgccagca tcagcgccag
     6721 cagggcgatc gcgcccagga acttgcccgc gacgatttcc gacgcccgca cgggcgacgt
     6781 catcagcagc tcgatcgtcc ccttccgcct ctcatccgtc atcagcccca tcgtcagcag
     6841 cgggaccgcg agcatgagca ggaacgcctc gttctggaag aactgcgcga gaacggttgt
     6901 cggcacgtcg agctggtagt ccgagcggcc caggcgctgg atctggctgt cggccagcgc
     6961 ggcctggcgg atgtagtgga cgagcatgtt cgagaagaac aagcccgaca cgaacgtgaa
     7021 gaccgccatc accgcggctg cgatcggcga gacgaagtac gactcgagtt cccgacgggc
     7081 gatggcgagc gcgtgccgca tcacgcgcct cccctgccgc cggccgtgcc gcccccgccg
     7141 ccggcctcgg cgctggcgcc gcctctcccg ctaccgctgc catcgccaac gcctttgctc
     7201 tcgccgccgc cctcgcgccc gcttccgtcc gtgagcgcga ggaagacgtc ttcgagcgac
     7261 agcgactcgc ccgcgaggcc gagcagcctc cagccgcgcg acacgacgag cgcggccgcc
     7321 tcggcgcgca ggtccgtgcc gcgcccgctc tcgacgcgcg cctcgatcgc gccggcgctg
     7381 tcggcgccag tgatgatgct cacgcgaatc gcgccgggca gggccgcgag cgcgagcgtc
     7441 acatcctccg gcatcggcgc aggcacgccc gcggccccgg cggccgttcc cgcgcccccc
     7501 accacgacgc gcaccgcgtc gcgcgcgcgc aggcgctcgc ccagccgctc gggcgtgtcc
     7561 tcggccatca cgcggccgcg gtgcaggatg acgatgcggt cggcgacgcc ggcgacttcg
     7621 ggcaggatgt gcgtcgaaag cacgaccgtg tgccggccct tgagctccag gatgaagtcg
     7681 cgcacctcgg cgcgctgctt cgggtcgagg ctggaggtcg gctcgtcgag gacgaggacc
     7741 ggcggctcgt ggacgatggc ctgggcgagg ccgacgcgct ggcggtagcc cttcgagagc
     7801 gtccggatcg ccgcgtccgc cgcgtcaccc agccgcgcgc ggccgatggc gcgatccacc
     7861 gccgcagccg tgcgcgcggc cggcacgtcc ttcagcgccg cgacgaaccg caggtagcgg
     7921 cgcaccgtca tctcgggatg gacgggcggc tgctccggca ggtagccgag caggcgtttc
     7981 gcgtcgatgg gccgctcgaa gacgtcgaag ccgccgatgc ggacgcgtcc gcgcgacggc
     8041 ggaacggagc ccgtgatcat gcgcagggtg gtggtcttgc cggcgccgtt cgggccgagg
     8101 aggccgagca cttc
//
LOCUS       DAOSIF010000078         8084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8284_length_8084_cov_4.458712, whole genome shotgun sequence.
ACCESSION   DAOSIF010000078 DAOSIF010000000
VERSION     DAOSIF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8284_length_8084_cov_4.458712"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLT35_04190"
     CDS             <1..236
                     /locus_tag="PLT35_04190"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOG28457.1"
                     /translation="GVRDALVEALYASGSDLLVLPVQDVFGWADRINVPATVGDSNWT
                     WKLPWFVDELDEQPEARERQRALRAWGQRHGRG"
     gene            565..1758
                     /locus_tag="PLT35_04195"
     CDS             565..1758
                     /locus_tag="PLT35_04195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG28458.1"
                     /translation="MLAGVLSAAACGREGAAPAGAAAGADRYLKPDTEEIAGTVARDA
                     TLSALLRAHLAPEHADAAVQVIARAMDPRRLRAGRPFTLTRTLDGRLRAFRCEIDADQ
                     YLRVEPPSPESPRDLAAEIVAYARREEPAVAEGRIDAAAPSLFQAMEAAGESVDLSIE
                     LAAIFSGEIDFNSELQPGDAFRLAFRRILRENGTVAYGPIAVAELDNGGRRLQAYRFV
                     PPGGQASYYDASGRSLKRFFLKSPLRFEPRITSRFSYSRRHPVLNVRRAHLGVDYAAP
                     AGAPVVAVAHGTVSRAGVAGDAGRLVAVRHASGYESMYLHLSSIAVRVGQRVAQGDLI
                     GRVGSSGLSTGPHLDYRLRKNGVYVNPITEHRRMPPGDPIPAALMADFLACRDRAAAL
                     LSPPR"
     gene            1936..3939
                     /locus_tag="PLT35_04200"
     CDS             1936..3939
                     /locus_tag="PLT35_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOG28459.1"
                     /translation="MTRSTRPEQPRSRAIAAAAPALLALLVLLVLPLGARQQAPANPD
                     RVRERYTKHEFRVPMRDGARLFTSVYVPKDRSKQYPFLISRTPYSVAPYGADSYRASL
                     GPSPQFEEEGFIFVYQDARGRYMSEGEFKQVRPHVPVKRGPRDVDESSDMHDTIEWLL
                     ANVAGHNGRAGLVGVSQGGFHVAAGMIDAHPALKAASPQAPTADYYLGDDVYHNGAFM
                     LAANFGFYSSFRPRDGGPQLPRAEPRFDPGNPDGYQFFLSIPPLAEVNAALFGGRAGY
                     WQEIVDHPTYDEFWQSRSIWRHAKGITPAVLHVGGWFDAEDPMGALRMYRAIERQSPG
                     TDNYLVMGPWSHGGWNRGPGDALGNATFAVKTGEVFRDRMQFAFFMHYLKDAPLPGGF
                     PKAWMFATGVNEFRRHDAWPPAGVRPVAMSLEPGGKLAGLGPAAPGTAASAPRAPRSD
                     AASFDEYVSDPNRPVPYIGYVAPGMTYDYMTEDQRFAATRPDVLVYASEPLAEDLTVA
                     GPVAVSLKASSSGTDCDFVVKLIDAYPYDYPQPGQEQSAAGRGGRPAVRPPSNEVRMG
                     GYQQLVRGEPFRAKFRRSFETPEPLEPGKPFEIRFELPDVYHVFRPGHRVIVQVQSSW
                     FPLVDRNPQQFMDIPKARPGDFRKATQRVYHGSTLTLLVESKR"
     gene            4753..5970
                     /locus_tag="PLT35_04205"
     CDS             4753..5970
                     /locus_tag="PLT35_04205"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOG28460.1"
                     /translation="MAKIRPFRALRPEPALASRIASVPYDVVSAEEARALAAGDELSF
                     LHVSRAEIDLPPGTDPYSDLVYRTAAERFARTRRSMVVEAEPALYFYRLRMGAHEQTG
                     LAACYSVDEYEAGLIKKHEKTRRDKEDDRTRHITELRAQTGPVFLVYAASPEVDAVAA
                     RVARTGPLYDLVAPDGVAHTVWKVTGADLDVLVAAFGRVPALYIADGHHRAASAARSR
                     KALAPEAGADGVVEADWFLAVAFPDDQTQVLPYNRVVKDLAGLTPEAFLDRVRGAFAV
                     AEGGPASPVRQGACAMYLGGRWYTLDIGARAGAGVVGRLDVSVLQDRLLAPILAIGDV
                     RTDKRIDFVGGIRGTSELERLVDEGKAAVAFSMYPVSVGDLMAIADAGEIMPPKSTWF
                     EPKLRDALLVHLI"
     gene            6120..7217
                     /gene="serC"
                     /locus_tag="PLT35_04210"
     CDS             6120..7217
                     /gene="serC"
                     /locus_tag="PLT35_04210"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOG28461.1"
                     /translation="MSSTPRVFNFAAGPAVLPLPVLEEAQRDLVSMPGVGMSILEMSH
                     RGKFFEGVLAQTEKDVRALAGVPDGYKVLFLHGGASLQFSMVPLNFLPRDASADYIVS
                     GAWSQKAVKEAKKVGGVKIAWTGEAGNFSTIPAQGDLELDPAAAYVSFTSNETIHGVE
                     FDQVPETGGVPLVCDASSDMFSRPIDVARHALIYAGAQKNIGPAGVALVIVSGEWLKR
                     RVEDPKLPSMLNYALHADNGSMYNTPPVFPIYIMGLVMKWLISIGGLPAIREINRRKA
                     AKLYAEIDRTGFYRGHAQKSCRSLMNVTFRLPSEELEAAFVKEAKAAGLDGLKGHRSV
                     GGLRASIYNAFPEAGIDALVEFMKEYERKHG"
     gene            7321..7707
                     /locus_tag="PLT35_04215"
     CDS             7321..7707
                     /locus_tag="PLT35_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG28462.1"
                     /translation="MVSWLTFLAIGLVAGVTAGLFGVGGGIVVVPALIYWAGFSQHKA
                     TGTSLAVLLPPIGLAAVLEYYRHGNVDVRAALLLIGGMFVGAWGGAILANRMRGPHLR
                     LAFGVFTCVMGVYLIHGALKRLGWFS"
     gene            complement(7832..>8084)
                     /locus_tag="PLT35_04220"
     CDS             complement(7832..>8084)
                     /locus_tag="PLT35_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VPDSG-CTERM sorting domain-containing protein"
                     /protein_id="HOG28463.1"
                     /translation="NPILNSLITVGGDQQYYFNLIGFSTDGGATIIPSFISQEGGSNS
                     AQLYGMVTAQQISVPDGGMTVALLGAALAGLGLIRRKLS"
BASE COUNT         1091 a         2905 c         2926 g         1162 t
ORIGIN      
        1 cgggggtgcg cgacgcgctc gtcgaagcgc tctacgcgtc cggatccgac ctgctcgtgc
       61 tgcccgtgca ggacgtgttc ggctgggcgg accgcatcaa cgtgccggcg accgtcggcg
      121 actcgaactg gacgtggaag ctgccgtggt tcgtcgatga actggacgag cagcccgagg
      181 cgcgggagcg gcagcgcgcg ctgcgcgcgt gggggcagcg gcacggaagg ggctaggggc
      241 taggcgctcg gcgctggtca ggcggggaca aggggcatag acatggggct agggaccagg
      301 cgctgggcgc tcgtcagtcc tgacgttgcg ctggacggcc ccccacgcgg cgcgagccgc
      361 gtccgactag cccctagcgc caagtcccta gtccctggcg gtgacccgct cccgggcggg
      421 cggcccgccg cacgcgggtg gccggggcgc tttccatgtg cccgcaggct agtcaccgtc
      481 agttctgccg aggacacatg gaaacgtccc ggacaggacc cgctcgcggg cgggtggccc
      541 gccgcacgcg ggtggctctg ccttgtgctc gccggagttc tgagtgccgc cgcgtgcggg
      601 cgtgaggggg cggcgccggc gggcgcggcg gcgggcgcgg accgctacct gaagcccgac
      661 accgaggaga ttgccggcac cgtcgcgcgc gacgcgacgc tgagcgccct gcttcgcgcg
      721 cacctcgccc ccgagcacgc cgacgccgcc gtgcaggtca tcgcgcgcgc catggacccg
      781 cgcaggctgc gcgcgggccg gccgttcacg ctgacgcgca cgctggacgg ccggctgcgc
      841 gcgttccgct gcgagatcga cgcggaccag tacctgcgcg tcgagccgcc gtcgcccgag
      901 tcgccgcgcg acctggccgc cgagatcgtg gcctacgcgc ggcgcgagga gccggccgtg
      961 gcggagggcc ggatcgacgc ggcggccccg tcgctcttcc aggcgatgga ggcggctggg
     1021 gagtcggtcg acctgtcgat cgagcttgcg gcgattttct ccggcgaaat cgacttcaac
     1081 agcgagctgc agcccggcga cgcgttccgc ctggcgttcc ggaggatcct gcgcgagaac
     1141 ggcaccgtgg cctacggccc gattgccgtt gccgagctcg acaacggcgg ccggcgcctg
     1201 caggcgtacc gcttcgtccc gcccggcggc caggcgtcgt actacgacgc gagcgggcgg
     1261 tcgctcaagc ggttcttcct gaagtcgccg ctcaggttcg agccgcgcat cacgtcgcgc
     1321 ttctcgtaca gccgccgtca ccccgtcctg aacgtccgcc gcgcgcacct cggcgtcgac
     1381 tacgccgccc ccgccggcgc acccgtcgtc gccgtggcgc acggcaccgt cagccgcgcc
     1441 ggcgtcgcgg gcgacgcggg ccgcctcgtg gccgtccgcc acgccagcgg ctacgagagc
     1501 atgtacctgc atctctcgtc cattgccgtg cgcgtcggcc agcgcgtcgc ccagggcgac
     1561 ctcatcggcc gggtcggatc ttcggggctg tccaccggcc cgcacctcga ctaccggctc
     1621 cggaagaacg gcgtctacgt caaccccatc accgagcacc gccgcatgcc gccgggcgac
     1681 cccattcccg cggcgctcat ggccgacttc ctggcctgcc gcgacagggc cgccgcgctc
     1741 ctttcgccgc cgcggtaggg gacgaggggc actgagggtg aggagcctgc gagcacgtgg
     1801 caagcgcccc ggccgcccgc gcgcggcggg cggacgcgaa gcgcggcgcg gcgtgctggc
     1861 gagcgggccc tcgagtccgg ccgttcggcg ccagcctgca gcacggcgcg ctggcgcgtc
     1921 ggctatactc gccggatgac tcgaagcacc aggcccgaac agccgcgctc tcgcgcgatc
     1981 gccgccgccg ctcctgccct gctcgccctg ctcgtcctgc tcgtcctgcc tctcggcgcg
     2041 cggcagcagg cgccggcgaa cccggatcgc gtccgcgagc gctacacgaa gcacgagttc
     2101 cgcgttccca tgcgcgacgg cgcgcggctg ttcacgtccg tctacgtgcc caaggatcgg
     2161 tcgaagcagt acccgttcct gatttcgcgc acgccgtaca gcgtcgctcc gtacggcgcc
     2221 gacagctacc gggcgtcgct cgggccgtcg ccgcagttcg aagaggaggg cttcatcttc
     2281 gtctaccagg acgcgcgcgg ccgttacatg tcggaagggg agttcaagca ggtgcggccg
     2341 cacgtgcccg tcaagcgagg cccgcgggac gtggacgaga gcagcgacat gcacgacacc
     2401 atcgagtggc tgctcgcgaa cgtggcgggc cacaacggcc gcgcgggcct ggttggcgtg
     2461 tcgcagggcg gcttccacgt ggcggcgggc atgatcgacg cgcacccggc cctcaaggcg
     2521 gcctcgccgc aggcgcccac ggccgactac tacctgggcg acgatgtgta tcacaacggg
     2581 gcgttcatgc tcgcggcgaa cttcggcttc tactccagct tcaggccgcg cgacggcggg
     2641 ccgcagcttc cgcgggccga gccgcgcttc gatcccggca atcccgacgg gtaccagttc
     2701 ttcctgtcga tcccgccgct cgccgaggtg aacgcggcgc tcttcggcgg gcgcgcgggc
     2761 tactggcagg agatcgtcga tcatcccacc tacgacgagt tctggcagtc gcgatccatc
     2821 tggcggcacg cgaagggcat cacgccggcg gtgctgcacg tgggcggctg gttcgacgcc
     2881 gaggacccca tgggcgcgct gcgcatgtac cgggcgatcg agcgccagag ccccgggacc
     2941 gacaactacc tcgtgatggg tccgtggtcg cacggcgggt ggaaccgcgg ccccggcgac
     3001 gcgctgggca acgcgacgtt cgcggtcaag accggcgagg tgttccgcga tcggatgcag
     3061 ttcgcgttct tcatgcacta cctgaaggac gcgccgctgc cgggcggctt cccgaaggcg
     3121 tggatgttcg ccaccggcgt gaacgagttc cgcaggcacg acgcgtggcc gccggccggc
     3181 gtgcgccccg tggcgatgtc gctcgagccg ggcgggaagc tggcgggcct cggcccggcg
     3241 gcgccgggca cggccgcgtc cgcgccgcgc gcgccgcgct ccgacgccgc aagcttcgac
     3301 gagtacgtca gcgatccgaa ccggcccgtc ccgtacatag gctacgtggc gcccggcatg
     3361 acgtacgact acatgaccga ggaccagcga tttgccgcca cgcgccccga cgtgctcgtt
     3421 tacgcgagcg agccgctcgc ggaggacctg accgtggccg ggcccgtcgc cgtgtcgctg
     3481 aaggcctcct cgtccggcac cgactgcgac ttcgtcgtga agctcatcga cgcctacccg
     3541 tacgactatc cgcagcctgg ccaggagcag tcggcggcgg ggcggggcgg caggcccgcc
     3601 gtgcggccgc cgtcgaacga ggtccgcatg ggcggctacc agcagctcgt acgcggcgag
     3661 ccgttccgcg ccaagttccg gcgcagcttt gagactcccg agccgctcga gcccgggaag
     3721 cccttcgaga tccgcttcga gctgccggac gtgtatcacg tcttccggcc gggccaccgg
     3781 gtgatcgtgc aggtgcagag ctcgtggttc cccctcgtgg atcgcaatcc ccagcagttc
     3841 atggacattc cgaaggcgcg ccccggggac ttccgcaagg cgacgcagcg ggtctatcac
     3901 gggtcgacgc tgacgctgct cgtcgagagc aagcgctaga cggattcggc gcgcggtgtc
     3961 cgcccgccgt atgcgggtag ccggggcgcg ttccgccggg gcccggcggc ggggatgacg
     4021 ccgcgcgggc ccgctcacgc tggccggcaa aacgacgctc cagggttccg tcggcggcgg
     4081 cgttgggtcc cggtcaccca acgcggactt cggcccttgc gcgccgtttt gcagactgac
     4141 gccgaacggc caggttcgag ggcctgctcg gcgtcacttc cgccgccctc ggtgtccgcc
     4201 cgccgcgcgc ggtgtccgcc cgccgcgcgc gggtagccgg ggcgcgttcc gccggggccc
     4261 ggcggcgggg atgacgccgc gcgggcccgc tcacgctggc cggcaaaacg acgctccagg
     4321 gttccgtcgg cggcggcgtt gggtcccggt cacccaacgc ggacttcggc ccttgcgcgc
     4381 cgttttgcag actgacgccg aacggccagg ttcgagggcc tgctcggcgt cacttccgcc
     4441 gccctcggtg tccgcccgcc gcgcgcggtg tccgcccgcc gcgcgcggtg tccgcccgcc
     4501 gcatgcgggt agccggggcg tgttccgccg gggcccggcg gcggggatga cgccgcgcgg
     4561 gcccgctgcc aggcgggcga ggcccccggc ccccgatctt gacttcccgc cttcgttctt
     4621 gctgaagtag cggggctggg cggcggcgag ccgctcatca ccggccccgg ggcacgtcgg
     4681 cactgagcgg ggctcctccg gccggcgccg cgaccggagc ggggacgcgg cggcgcggac
     4741 tgcgagcgca gcatggcgaa gatcaggcct ttccgcgcgt tgaggccgga gcccgcgctc
     4801 gcctcgcgca tcgcctcggt tccctacgac gtcgtgagcg ccgaggaggc gcgcgcgctg
     4861 gccgccggcg acgagctgag cttcctgcac gtgtcgcgcg ccgagatcga cctcccgccc
     4921 ggcaccgacc cgtactcgga tctcgtctac cggaccgcgg ccgagcgctt cgcccggacg
     4981 aggcggtcga tggtggtgga ggccgagccg gcgctgtact tctacaggct gcggatgggc
     5041 gcgcacgagc agaccgggct tgccgcgtgc tactcggtgg acgagtacga ggcgggcctc
     5101 atcaagaagc acgagaagac gcgccgggac aaggaagacg accgcacgcg ccacatcacc
     5161 gagcttcgcg cgcagaccgg gcccgtgttc ctcgtctacg ccgcctcgcc cgaggtcgac
     5221 gccgtcgccg cgcgggtggc gcggaccggg ccgctctacg acctcgtcgc cccggacggc
     5281 gtcgcgcaca ccgtctggaa ggtcaccggc gccgacctcg acgtcctggt cgcggcgttc
     5341 ggccgcgtgc cggcgctgta catcgccgac ggccaccacc gcgcggccag cgccgcccgg
     5401 tcgcgcaagg cgcttgcgcc cgaggcgggc gcggacggcg ttgtcgaggc cgactggttc
     5461 ctcgccgtgg ccttcccgga cgaccagacg caggtgctgc cgtacaaccg cgtcgtgaag
     5521 gacctggcgg ggctgacgcc cgaggcgttc ctcgaccgcg tgcgcggcgc cttcgccgtc
     5581 gccgagggcg gcccggcgtc gcccgtgagg cagggcgcgt gcgcgatgta cctcggcggg
     5641 aggtggtaca cgctcgacat cggcgccagg gcaggcgccg gtgtcgtcgg ccgcctcgac
     5701 gtcagcgtgc tgcaggatcg cctgctcgcg ccgatcctgg cgatcggcga cgtccgcacc
     5761 gacaagcgca tcgacttcgt cggcgggatc cgggggacga gcgagctgga gcggctggtg
     5821 gacgaaggga aggccgccgt ggcgttttcg atgtaccccg tgagcgtggg cgatctgatg
     5881 gccatcgccg acgccggcga gatcatgccc ccgaagtcca cctggttcga gccgaagctg
     5941 agggacgcgt tgctcgtgca cctcatctag gggccggggg ccggggagcg gggccccggg
     6001 gcgcggcggc ggcggcgatc gagtccggca acccgcgcgt tccgcacgcg cacgtcgtca
     6061 tgtccgtgga ccgatcgcgg gccccgcccg cgccgcggat cgagatcgag gaggcagtca
     6121 tgtcgtccac tccacgcgtg ttcaacttcg ccgccggccc ggccgtgctg ccgctgcccg
     6181 tgctcgagga ggcgcagcgc gacctggtga gcatgcccgg cgtcggcatg tccattctcg
     6241 agatgagcca ccgcggcaag ttcttcgagg gcgtgctcgc gcagaccgag aaggacgtcc
     6301 gcgcgctggc gggcgtgccg gacggctaca aggtgctgtt cctgcacggc ggcgccagcc
     6361 tgcagttctc gatggtgccg ctgaacttcc tgccgcgcga cgcgtcggct gactacatcg
     6421 tgtcgggcgc gtggtcgcag aaggccgtga aggaggcgaa gaaggtcggc ggcgtgaaga
     6481 tcgcgtggac gggcgaggcc ggcaacttca gcaccatccc cgcccagggc gacctggagc
     6541 tcgaccccgc tgccgcgtac gtgtcgttca cgtcgaacga gaccatccac ggcgtcgagt
     6601 tcgaccaggt gcccgagacc ggcggcgtgc cgctcgtgtg cgacgcctcg tcggacatgt
     6661 tcagccggcc catcgacgtg gcgaggcacg cgctcatcta cgccggcgcg cagaagaaca
     6721 tcggccctgc cggcgtggcg ctcgtcatcg tgagcggcga gtggctgaag cggcgcgtcg
     6781 aggatccgaa gctgccctcg atgctcaact acgccctcca cgccgacaac ggatcgatgt
     6841 acaacacgcc gcccgtgttc ccgatttaca tcatgggcct cgtgatgaag tggctgatct
     6901 cgattggcgg cctgccggcc atccgcgaga tcaaccggcg gaaggccgcg aagctctatg
     6961 ccgagatcga ccgcaccggc ttctaccggg ggcacgcgca gaagagctgc cggtcgctga
     7021 tgaacgtgac gttccggctg ccgagcgagg agctggaggc ggcgttcgtc aaggaagcca
     7081 aggcggccgg cctcgacggc ctcaaggggc atcgctccgt gggcggcctg cgcgcgtcca
     7141 tctacaacgc gttcccggaa gctggcatcg acgcgctcgt cgagttcatg aaggagtacg
     7201 agcgcaagca cggctagggg tcgggacgat tttcgcaacg ggcctgcggc cagctacaat
     7261 cctgttggcg cgccggcccc ttcggccttc gaagagaggc cggcctgctc gggagggcgc
     7321 atggtttcgt ggctgacctt cctcgccatc ggcctggtcg ccggcgtcac cgccggcctc
     7381 ttcggcgtcg gcgggggcat cgtcgtcgtg cccgcgctca tctactgggc cggcttctcg
     7441 cagcacaagg ccaccgggac cagcctggcc gtcctgctgc ctcccatcgg cctcgctgcc
     7501 gtcctcgagt actaccggca cggcaacgtg gacgtccggg cggcgctgct cctcatcggc
     7561 ggcatgttcg tcggggcgtg gggcggggct atcctggcca accgcatgcg cgggccgcac
     7621 ctgcggctgg ccttcggcgt cttcacctgc gtcatgggcg tctatctcat tcacggcgcc
     7681 ctcaagcggc tggggtggtt ctcgtagcac gcttgcctgc ggcgggctct ggagaagcga
     7741 ggcccgagcc tacctgcggc gccgcccaga agaagaaaag gcagggcgcg tgcgccctgc
     7801 ctttcagaca gcgcaagccg gagcggcgag actaggacag cttgcggcga atcaagccaa
     7861 gaccggcaag cgcggccccg aggagcgcga ccgtcatgcc gccatcggga accgagatct
     7921 gctgagccgt gaccatcccg tacagctgag cggagttgct cccgccctcc tgagaaatga
     7981 acgacgggat gatggtcgcc ccaccatccg tgctgaagcc aatcagattg aaatagtact
     8041 gctgatcccc tccgaccgtg atcagcgagt tcaggatcgg gttc
//
LOCUS       DAOSIF010000079         8042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8337_length_8042_cov_4.266558, whole genome shotgun sequence.
ACCESSION   DAOSIF010000079 DAOSIF010000000
VERSION     DAOSIF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8337_length_8042_cov_4.266558"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLT35_04225"
     CDS             <1..973
                     /locus_tag="PLT35_04225"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOG28464.1"
                     /translation="LQDERSDADGEIRRGVGLMADLTVRIGSLVLKNPLVSASGCFGY
                     GLEYAGIVDLSSLGAVVVKGLFLEEREGHPPPRVVETPSGLLNAIGLQGIGARRFVRE
                     KLPELRRLNAVVVVNVCGTTIDEYAEVSRILSDAEGVAALELNISCPNVREGGIQFGS
                     DLASAGAVVAAVRKVTPLPIFPKLTPNVTSVAAFARAAEENGADAVSLVNTFLAMVVD
                     VERRRPVLSNGMGGLSGPAIRPIAVRMVYECAQQVRIPVIGMGGIASARDALEFLIAG
                     ASAVQVGTANFVDPFIWSALAAGIEDYMIRHGIARLADLVGTLERGR"
     gene            1002..1745
                     /gene="pyrF"
                     /locus_tag="PLT35_04230"
     CDS             1002..1745
                     /gene="pyrF"
                     /locus_tag="PLT35_04230"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOG28465.1"
                     /translation="MHPENPVIVALDVESPSRALALAGELRGVVGAVKVGSQLFTAAG
                     PDIVRRLAEGGHRVFLDLKYHDIPNTVAGAAAEAATLGAWMIDVHASGGLSMIKAARE
                     AAHAGAVRAGVAPPLVVAITVLTSFDENTLASVGVRGTVIDQVDALAWLAQEAGADGV
                     VASAHEVARLRSARGDGFVLVTPGIRPAHAPGAPRPKDDQARTMTAAQALAAGATYIV
                     VGRPIIAAQDPRAAAEAMAAEAMAASPRR"
     gene            complement(1785..2354)
                     /locus_tag="PLT35_04235"
     CDS             complement(1785..2354)
                     /locus_tag="PLT35_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG28466.1"
                     /translation="MKRLLPAFILVAAAALLVMSAACAKKAPVAAPPPPPPAPTAPPP
                     PPPPPPPPPPPPAPKPLTEEEIFAKKTLAELNAEQPLQDVFFDYDKADLRPDALTSLQ
                     RNAEWLRRWTSTKIVIEGHCDSRGTNEYNLALGDKRASAVRAYLTGLGIPADRLSSVS
                     KGEESPFCTEETESCWSQNRRGHFIITAK"
     gene            2486..3235
                     /locus_tag="PLT35_04240"
     CDS             2486..3235
                     /locus_tag="PLT35_04240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG28467.1"
                     /translation="MDLLLEATALAEQHHFWFRGFRRFAAPLLKQAASGRRGLRILDC
                     GCGTGYNIRSLLEPCGAAWGVDLAPTGLEHARRAGVARLARASVTALPFAPASFDIAT
                     SFDVLYALPDEAERAAVAEMWRVLKPGGAAVVNVAAMPVLRGHHSVLAAEIRRYTPAA
                     LRALLERTGFRLERMRYTHAAVFPLLLAVRSVQRAAGPVRPDEALADLIVPAAPINAA
                     LSGLLAVEAAVSRAVPMPFGSSLLCLARKPA"
     gene            complement(3171..5576)
                     /locus_tag="PLT35_04245"
     CDS             complement(3171..5576)
                     /locus_tag="PLT35_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOG28468.1"
                     /translation="MTDRLASVRRNPRAWRVAAVLALFFAAGHLPFLASTLEDVDSAN
                     FALGVRDFAPGLHRPHPPGYPIYIALGKAAESVLSEPHALSIWGVLFGALSAFALVRL
                     FGALDGLDGEPAPVLSRVTPRRFGEWLGRPAVATALTLFAPLYWLTASRPMSDSAGVA
                     LSLAAQAVLVTAMVRQREVFADRSGSVDAAAAASAGRLVLAGAFVSALAFGFRSQGAW
                     LTLPLLALVVFDRAGRGAAAALIGATVWFVTGTLLWLVPLIVASGGPAEYLAALGSQA
                     GEDWTGVDLLVTNFTARGLAHSLYDTIIRHWAGIGWLMTALAALGAAVLLVRGRRALA
                     VIVAAYAPYAIFHLAFQETCTTRYALPLVAPMAYLAARGLFALRPIGGSTIAACLAAA
                     LTFNALPATAAYSRAGSPVVRALADARAAAERSPGIAVGMHHPFARLVEAEGLGRAAA
                     LPAPPGHEWLELVKRWRAGAAGPVWYFADPRRTDLALVDRQAQRVRGEYSWPIPTPVY
                     LGGIRPDALRWVEIDSPGWFAGEGWELTPETAGVARREHRGLEQGPITAWVRPRAGAA
                     TAMIGGRHLGAGTGPDALVSLRVDGREIERWTATAASPSFLRFVPLPPGSLAGSAPWS
                     VLEIHAATGDGRPTGIVAIDQFDLQDDPASPMLGFGEGWQESEHAPRQGLSWRWASGR
                     AVLRVWPADRPVDLEIAGESPRRYFGRASRVQARAGAVTVWRLETGDDFTAVITVPAE
                     ALRAAGGAVAIETDQTFRPADRGQGADRRELGLRIFRVTIVTPASSQGRAANCRTASG
                     RPG"
     gene            complement(5585..6445)
                     /gene="larE"
                     /locus_tag="PLT35_04250"
     CDS             complement(5585..6445)
                     /gene="larE"
                     /locus_tag="PLT35_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOG28469.1"
                     /translation="MTDSTSQAAPAPDERLLVQKEASLRAAIAGYESVVVAFSGGTDS
                     AYLGWVARDVLGDRALCVTADSPSLPSRHRRRALELARAFALPHEFIITNEMDLPEYT
                     ANEPDRCYHCKRELFSRLAELARARGIAIVADGANADDRGDYRPGRRAARELGVRSPL
                     DEAGLAKADIRELSRRAGLPTWDEPASACLSSRIPYQSAVTPEKLAAIERAEDVLAGL
                     GFRQYRVRHHGDVARIEAGPDDFPRMLEPAVREALVRELKLVGFRYVALDLQGYRTGS
                     LNEGVRLKPV"
     gene            complement(6442..6660)
                     /locus_tag="PLT35_04255"
     CDS             complement(6442..6660)
                     /locus_tag="PLT35_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28470.1"
                     /translation="MAICPECEFEIDVDEYDVDRGDIVECGNCGTNLEVVGLNPIEFD
                     VASDDEDDEELDEDDLDEGGDEPDEDDE"
     gene            6869..7579
                     /locus_tag="PLT35_04260"
     CDS             6869..7579
                     /locus_tag="PLT35_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG28471.1"
                     /translation="MTGAVHKTVVIADDTPFVRDRFKAAIEHAGHRALVVRSAAELLA
                     RVRADLPNLDLVVVDLRLPHAGGVDLVRAIRKLDGGRLPIMVFSGTIAGASEIRDLAN
                     LGIAGYVNEYSPSPQILPALAPHLFPDSFNRRSGPRVSLGIPVAYRFGNTIAAAVTLN
                     LGRGGLAIRTMSPLETSTRARVRFRLPNAKRDVDADTSVAWSDRRVGMGMQFERIDAA
                     DQSAVEEFVDAQYLQHAN"
     gene            7786..>8042
                     /locus_tag="PLT35_04265"
     CDS             7786..>8042
                     /locus_tag="PLT35_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG28472.1"
                     /translation="MIEVQNLTKRYGRVTAVDDVSFRVERGEILGFLGPNGAGKTTTM
                     RVITGYMPPSEGRVRIAGFDTLEQPIEAKRRTGYLPESPPL"
BASE COUNT         1160 a         2970 c         2823 g         1089 t
ORIGIN      
        1 tcttcaggac gagcgatccg atgcggacgg tgagatccgc cgtggcgtgg gactgatggc
       61 ggatctcacc gtccgcatcg gatcgctcgt cctgaagaac ccgctcgtga gcgcgagcgg
      121 gtgcttcggt tacggcctcg agtacgccgg tatcgtcgat ctctcctcgc tcggcgccgt
      181 ggtcgtcaag gggctgttcc tcgaggagcg ggaagggcac cccccgcccc gggtggtgga
      241 gacgccgtcg ggcctgctga acgccatcgg cctgcagggc atcggcgcgc gccgcttcgt
      301 gcgcgagaag ctcccggagc tgcgccgcct gaacgccgtc gtcgtcgtca acgtctgcgg
      361 gaccaccatc gacgagtacg cggaggtctc gcggatcctg tcggatgccg agggcgtggc
      421 ggccctcgag ctgaacatct cgtgcccgaa cgtcagggaa ggcggcatcc agttcgggtc
      481 cgacctggcc agcgccggtg cggtcgtggc ggccgtccgg aaggtgacgc ccctgccgat
      541 ttttccgaag ctgacgccga acgtcacgag cgtggcggcc tttgcgcgcg cggccgagga
      601 gaacggcgcc gacgccgtgt cgctggtgaa cacgttcctg gcgatggtcg tcgacgtcga
      661 gcgccggcgg cccgtcctct ccaacggcat gggcgggctc agcggccccg ccatccgccc
      721 gatcgcggtc cgcatggtct acgagtgcgc gcagcaggtc cgcattccgg tcatcggcat
      781 gggcgggatc gccagcgccc gcgacgccct cgagttcctc atcgccgggg cgtccgcggt
      841 gcaggtgggc accgcgaact tcgtcgatcc gttcatctgg tcggcgcttg ccgccggcat
      901 cgaggactac atgatccgcc acgggattgc gcggctcgcc gatctcgtcg gcacgctgga
      961 acgcggccgc tgacggccgc gccggcccgg aggatgaggc gatgcacccg gagaaccccg
     1021 tcatcgtcgc gctggacgtg gaaagcccgt cgagggcgct ggccctcgcc ggcgagcttc
     1081 gaggcgttgt cggcgccgtc aaagtcggca gccagctctt caccgcggcc gggcccgaca
     1141 tcgtccggcg cctcgccgag ggcggccacc gcgtgttcct ggatctcaag taccacgaca
     1201 tcccgaacac cgtggcgggc gcggccgccg aggcggccac gctcggcgcg tggatgatcg
     1261 acgtgcacgc aagcggcggc ctgtcgatga tcaaggccgc gcgggaggcg gcgcacgccg
     1321 gcgccgtccg ggcgggcgtc gcgccgcccc tggtggtggc catcacggtg ctgaccagct
     1381 tcgacgagaa caccctcgcg tcggtgggcg tgcgcggcac ggtcatcgac caggtggacg
     1441 cgctcgcctg gctcgcgcag gaagcgggcg ccgacggcgt cgtggcgtcg gcgcacgagg
     1501 tggcgcgcct gcggtcggcg cgcggggacg ggttcgtgct ggtgacgccc ggcatccggc
     1561 cggcccacgc cccgggcgcg ccgcgcccga aagacgacca ggcgcgaacg atgaccgccg
     1621 cgcaggccct cgccgccggc gcgacctaca tcgtcgtcgg gcggcccatc atcgccgcac
     1681 aagacccgcg ggccgccgcc gaggccatgg ccgccgaagc gatggccgcc agcccccgcc
     1741 gctgagcgga cgggcgccgg cggcccgcag ccgtgaaggc cggcctactt cgccgtgatg
     1801 atgaagtgcc ccctgcggtt ctgcgaccag cagctctccg tctcctcggt gcagaacggc
     1861 gactcctcgc ccttgctgac cgacgagagg cgatccgccg gaatgccaag ccccgtcagg
     1921 taggcgcgaa ccgccgaggc gcgcttgtcg ccgagcgcga ggttgtactc gttggtgccg
     1981 cgcgagtcgc agtgcccctc gatcacgatc ttcgtggagg tccagcgcct cagccactcc
     2041 gcgttccgct gcagtgacgt gagcgcgtcc ggacgaaggt ccgccttgtc gtaatcgaag
     2101 aagacgtcct ggagcggctg ttccgcgttc agttccgcga gcgtcttctt cgcgaagatc
     2161 tcctcttccg tcagcggctt cggcgccggc ggcggcggcg gcggcggcgg cggaggaggc
     2221 ggcggcggcg gtgcagtcgg cgccggtgga ggcggcggtg gagccgcaac aggagccttc
     2281 ttcgcgcacg cggcggacat gacaagcagc gcagccgcag ctacgaggat gaatgccggc
     2341 agcagtctct tcatcaccca atatcctccc ggggggcgct ctcttacgga ctctagccga
     2401 agagcggggt aggacacaat cggaaccggc ctgccccggt cgcgcggccg ccggccggga
     2461 accggcgaca gcatacaata cgcgcgtgga cctcctgctg gaagcgaccg cccttgcaga
     2521 gcagcatcac ttctggttcc gcgggttccg ccgcttcgcc gcccccctgc tgaagcaggc
     2581 cgcctcgggc cggcgcggcc tgcgcatcct cgactgcgga tgcggcaccg gctacaacat
     2641 ccgcagtctg ctcgaaccct gcggcgcggc gtggggcgtg gacttggccc caaccggact
     2701 cgagcacgca cggcgggccg gcgtggcccg actcgcccgc gccagcgtga cggccctgcc
     2761 cttcgcaccg gcgtccttcg acatcgccac gtccttcgac gtgctgtatg ccctgccgga
     2821 cgaggccgag cgcgcggcgg tcgccgagat gtggcgcgtc ctcaagccgg gaggggcggc
     2881 cgtcgtcaac gtcgcggcaa tgcccgtcct gcgggggcat cactccgtgc tggcggccga
     2941 gatccgccgc tacacccccg cggcccttcg cgccctgctc gagcgaaccg gcttccgcct
     3001 cgagcggatg cggtacaccc acgccgccgt gttcccgctg ctcctcgcgg tgcgcagcgt
     3061 gcagcgcgcg gcggggccgg tgcgccccga cgaggcgctg gccgatctga tcgtgccggc
     3121 ggcgccgatc aacgcggcgc tgtcgggcct cctcgccgtc gaggcggccg tcagccgggc
     3181 cgtcccgatg ccgttcggca gttcgctgct ctgccttgcg aggaagccgg cgtgacgatc
     3241 gtcacgcgaa agatccggag gccgagctcc cgccgatccg cgccctggcc ccggtcggca
     3301 gggcgaaaag tctggtcagt ctcaatcgcc accgccccgc ccgccgcccg caacgcctcg
     3361 gccgggacgg tgatgacggc ggtgaagtcg tcgcccgtct cgaggcgcca caccgtgacg
     3421 gcgccggccc gagcctgaac acgcgaggcg cgcccgaagt accgtcgagg cgattcgccc
     3481 gcgatctcca gatcgacggg cctgtccgcc ggccacacgc ggagcaccgc ccggccgctc
     3541 gcccacctcc acgagagccc ctgccgcggc gcgtgctcgg actcctgcca gccctcgccg
     3601 aatcccagca tcggcgacgc cggatcatcc tgcaggtcga actgatcgat ggccacgatg
     3661 cccgtaggac gcccgtcgcc ggtcgccgcg tggatctcga gcaccgacca gggcgcgctg
     3721 ccggcgagcg agccgggggg gagcggcacg aaccggagga acgacgggct cgccgccgtc
     3781 gcggtccacc gctcgatctc ccggccgtcc acgcgcaggc tcacgagcgc gtcggggcct
     3841 gttccggccc cgagatggcg gccgccgatc atggccgtcg ccgcgccggc gcgaggccgc
     3901 acccacgcgg tgatggggcc ctgctccagg ccgcgatgct cgcgccgggc cacgccggcc
     3961 gtttccggcg tcagctccca gccttcgccg gcgaaccagc cgggggaatc gatttccacc
     4021 caccgaagcg cgtccgggcg gatgccgccg aggtacacgg gcgtcgggat gggccacgaa
     4081 tactcgccgc gcacgcgctg ggcctgccgg tccaccagcg cgaggtccgt gcgccgggga
     4141 tcggcgaaat accataccgg tccggctgcg cccgcccgcc agcgcttcac cagctcgagc
     4201 cactcgtgcc ccgggggcgc cggcagcgcc gctgcccgcc cgagcccctc ggcctcgacg
     4261 agccgggcga aggggtgatg cataccgacc gcgatccccg gcgagcgctc cgccgctgct
     4321 ctcgcgtccg ccagcgcgcg cacgaccggg ctgcccgccc gcgagtaggc ggctgtggcg
     4381 ggcagcgcgt tgaacgtgag cgcggcggcc aggcacgcag cgatggtcga cccgccgatg
     4441 gggcgcagcg cgaacaggcc gcgcgccgcc aggtaggcca tcggtgccac aagcggcagc
     4501 gcgtaccggg tcgtgcacgt ctcctggaac gccaggtgga agatcgcgta cggcgcgtag
     4561 gcggcgacaa tcaccgccag ggccctccgg ccccgcacga gcagaaccgc cgcgccgagg
     4621 gcggccaagg ccgtcatgag ccacccgatg ccggcccaat ggcggatgat cgtgtcgtag
     4681 agggaatgcg ccaggcccct ggcggtgaag ttcgtgacga gcaggtcgac gcccgtccaa
     4741 tcctcgcctg cctggctgcc cagggccgcc aggtactcgg cgggcccgcc gctcgccacg
     4801 atgagcggca cgagccacag gagcgtgccg gtgacgaacc agaccgtggc gccaatgagc
     4861 gcagccgcgg cgccgcggcc cgcgcgatcg aacaccacga gggccagcag cggcagcgtc
     4921 agccaggccc cctgcgagcg gaacccgaat gcaagcgccg agacgaaggc gcccgcgagg
     4981 accagccggc ccgccgacgc tgccgccgcg gcatcgacgc tcccggagcg atccgcgaac
     5041 acctcgcgct ggcgaaccat ggccgtgacc agcaccgcct gagcggcgag ggacaacgca
     5101 acaccggccg agtcgctcat cgggcgcgac gcggtcagcc agtacaacgg cgcgaatagt
     5161 gtcagggcgg tcgccacggc gggccggccc agccactccc cgaaccgccg cggcgtgacg
     5221 cggctgagaa ccggtgccgg ctccccgtcc agcccgtcca gcgccccgaa gagccgcacc
     5281 agcgcgaacg ccgacagcgc cccgaacagc acgccccaga tcgagagggc gtgcggctcg
     5341 ctgagcacgc tctcggcggc cttcccgagc gcgatgtaaa tcgggtagcc cggcgggtgc
     5401 gggcggtgga ggcccggggc gaagtcgcgc acgccgaggg cgaagttcgc cgaatcgacg
     5461 tcctcgaggg tggacgccag gaacggcagg tgcccggcgg cgaagaacag ggccaggaca
     5521 gccgccaccc gccacgcgcg ggggttgcgt cggactgacg ccagacgatc cgtcacgcgt
     5581 cgcctcagac cggcttgagc cggacgcctt cgttcagact cccagtcctg tatccctgga
     5641 gatcgagcgc gacgtaccgg aatccgacga gcttcagctc gcgcacgagg gcttcgcgca
     5701 cggccggttc cagcatgcgc gggaagtcgt cgggcccggc ctcgatgcgc gccacgtcac
     5761 cgtggtgccg gacgcggtac tgccggaagc cgaggccggc aagcacgtcc tccgcccgct
     5821 cgatggcggc gagcttctcg ggggtcaccg ccgactggta gggaatgcgc gaggacaggc
     5881 acgccgacgc cggctcgtcc caggtcggga gccccgcgcg tctcgacagc tcgcggatgt
     5941 ccgccttggc caggccggcc tcgtcgagcg ggctgcgcac gcccagctcg cgagcagccc
     6001 ggcggccggg ccggtagtcg ccccggtcgt cggcgttggc gccgtcggcc acgatcgcga
     6061 tcccgcgggc ccgcgccagc tccgccaggc gggagaacag ctcccgcttg cagtggtagc
     6121 accggtccgg ctcgttggcg gtgtactccg gcagatccat ctcgttcgtg atgatgaact
     6181 cgtgcggaag agcgaacgcc cgggcgagtt caagggcgcg ccggcggtgg cgggacggca
     6241 ggctcggact gtcggccgtc acgcagagcg cccggtcgcc gagaacgtcc ctcgccaccc
     6301 atccgaggta ggcgctgtcg gtgcccccgc tgaatgccac cacgaccgac tcgtagccgg
     6361 cgatggccgc gcgcaacgag gcttctttct ggaccagcag gcgctcgtcg ggcgcgggcg
     6421 cggcctggga ggtcgaatcc gtcactcgtc gtcctcgtcc ggctcgtccc cgccctcgtc
     6481 gagatcgtcc tcgtccagtt cctcgtcgtc ctcgtcgtcg gaggcgacgt cgaactcgat
     6541 gggattgagg ccgacaacct ccaggttggt tccgcagttg ccgcattcca cgatgtcgcc
     6601 ccgatcgacg tcgtactcgt ccacgtcgat ctcgaactcg cactcagggc agatcgccat
     6661 accgcctcct caacggatcg ggcgaggtcg cgcgtcggcc cccaggccgc gggtccgccg
     6721 cgccttcgta actgctgaca caccgacgtt tgtggcaaca ggagccagga ttatagcggc
     6781 aaggcccccg cgctgcaacg aaccgcgagg cttgcgcggg ccctatcgtc ccggctccgc
     6841 ccggccgata cacctgcacg gaatgaccat gacgggagcc gtccacaaga ccgtcgttat
     6901 cgccgatgat accccgttcg ttcgggatcg gttcaaggcc gccatcgagc acgccgggca
     6961 ccgggcgctc gtcgtgcgga gcgccgccga gctgctggca cgtgtccggg ccgacctgcc
     7021 gaacctcgac ctggtggtgg tggatctccg gctgccccac gccggcggcg tcgatctggt
     7081 gcgggcgatc cgaaagctcg acggcgggcg cctgccgatc atggtcttca gcggcaccat
     7141 cgccggcgct tctgagattc gcgacctggc gaacctgggc attgcgggat acgtgaacga
     7201 gtacagcccg tccccgcaga tcctgcctgc cctcgcgccc catctctttc ccgacagctt
     7261 caaccgccgg agcggcccgc gcgtgtcgct cggcatcccc gtggcctacc gcttcggcaa
     7321 caccattgcc gccgccgtca cgctgaacct cgggcgcggc ggcctcgcca tccgcacgat
     7381 gagcccgctc gagacctcga cgcgcgcgcg cgtccgcttc cggctgccga acgccaagcg
     7441 tgacgtggac gcggatacgt ccgtcgcctg gagcgaccgg cgcgtgggca tgggcatgca
     7501 gttcgagcgg atcgacgccg ccgaccagtc ggccgtcgag gagttcgtgg acgcgcagta
     7561 cctccagcac gcaaactgag cccgaggagc cgggacgccc gccccctgag cgctcacgtg
     7621 gggtcagatc tggggtcaga tccagtaata ctggatctga ccccatattg acactgtctc
     7681 cgtggcgtcg gtacgatgag tggctggcgc cttgcgggac gagccgagca gcccccgcgt
     7741 ctccggccgg cccgctcgta gtcaccacac ttcctggagc acgcagtgat cgaggtccag
     7801 aacctcacga agcgctacgg gcgcgtcacg gccgtcgacg acgtgtcgtt ccgcgtcgag
     7861 cgaggcgaaa tcctcggctt cctcggaccg aacggcgccg gcaagacgac gacgatgcgc
     7921 gtcatcaccg ggtacatgcc gccgtcggag ggccgcgtcc gcatcgcggg cttcgacact
     7981 ctcgagcagc ccatcgaggc gaaacggcgc accggctatc tgcccgagtc gccgccgctc
     8041 ta
//
LOCUS       DAOSIF010000080         7916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8490_length_7916_cov_4.060298, whole genome shotgun sequence.
ACCESSION   DAOSIF010000080 DAOSIF010000000
VERSION     DAOSIF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8490_length_7916_cov_4.060298"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PLT35_04270"
     CDS             <1..992
                     /locus_tag="PLT35_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28473.1"
                     /translation="PMHNDRLSRREWLRAAGGGAVLSAIPFRARPASAQAGGRPNSKV
                     AGVQLGVTTYSYLSMPHKVDDVIQYLLYAGVNSIELRSVAEEDLGIPQAAGPAGGGAP
                     LTEQEKAERARAAESLRNAQRAWRLSLPMERYARMRRKFNDAGIDVHIAKFAPSSWSD
                     EEIDYAYTAAKTLGSIGITDELSEQACQRLGRFAEKHRSLAMFHTHGQVADPGFSFDK
                     YLAYSPANMLNLDAGHYYGATGLHPGDVIVKYHERMRSIHIKDKTGPRHATPNTNKPF
                     GQGETPIADILLLLKKEKWPIEVDVELEYPIPDGSDAARETKKCIDYLRNILE"
     gene            complement(1128..1841)
                     /locus_tag="PLT35_04275"
     CDS             complement(1128..1841)
                     /locus_tag="PLT35_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG28474.1"
                     /translation="MKPLLSIIVPVYNEEATIGAVLDRLLGVPFPVPIEIIAVNDGSH
                     DGTRQALDAAAARSPLITAVHLESNRGKGAALRTGFARSAGAIVAIQDADLELDPAQL
                     PALVAPILEGGADVVYGSRFRGGSSRLPLMSRLGNAGLTMLTNLLYGARLTDMETCYK
                     IMRGDIARGLRLTADRFDIEPEITVNLLRSGVRILELPVTSLSRSRRAGKKIRWRDGW
                     HAAALLVRRRFRPRPQRPL"
     gene            complement(1853..3502)
                     /locus_tag="PLT35_04280"
     CDS             complement(1853..3502)
                     /locus_tag="PLT35_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28475.1"
                     /translation="MSLLAPAVLTPLAAYAADLAGVAFTPLPMAALAAMAFLFCGALL
                     FRRAKPDGVGLMLGAAAGALLLAWLLWLSAPSLLPLGTGPDLTHHLMLVRHIETHWRL
                     VHDPALEQSLGEMAHYTPGSHILVALAGAWSGTDGLRAMHGVQAWTVALKAVFLVAVC
                     LRLLPPRAPRLLAALGPVLLLESPRYFLGGFTEFGFLAQVVAELPAVAMWWALVAWAD
                     SRADLALGVFGAAGAAAFLAWPVYVGPPALAAGVLIASRGRRPFARRVRDLAVAFAPL
                     ALVAAAFAIGRLGWLRLAGTGGLAPWPSIAAYGRPLVVLSATGLLVAAARERGRATAG
                     FALAVLIQAAALCLVARRAGAPQPYMALKMGYLLLWPMAACAVAAAGEAWAWLDRTVR
                     VSRAWGNAVALAVVAAALALAARPLIAKPRLLHPRPPAVSLPLYEAGRWARSHLPPSC
                     VEYLVGDDETAYWLHLAVLGNPRASARSADNSTYEPADAIVRWLTPNGLPYALVDLPA
                     LPRDVRAELDVMQSFGTAAIARRRGPSACAAAPRADSPPIR"
     gene            3646..5292
                     /locus_tag="PLT35_04285"
     CDS             3646..5292
                     /locus_tag="PLT35_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28476.1"
                     /translation="MEQLAPPTADASRGGFPRPRPPRRSGIQLVVLFAAVMAAVCVAV
                     IVATAPASWPRSDLAVIEIDVLHALSTFWKLGPYSRFGWRHPGPLHFYLLAPLYWLSG
                     EKSAALPAGAFLLNLGSVGFVAWAARRHLRPGSAVGVTLALAALATRADGLLTSVWNP
                     HAVVLPLAATMTAAALACADHAGFLVAAVLGASLIAQTHVGLVPVALAAVAAPAGAMA
                     ANLVRDGCEARPSTRAALRALAVSIALGLLLWTPPLLEQLEGRPGNLAQVAAYFTSAE
                     RTGQPVAAAARAVGYEFSWIWRPRPALPDGKPLRRQPARAWVVWSGSQLAAIAALLVA
                     WRHGRRDPRWWLGVVTLLAASMGLASATRVDPPAPDHALFWLAAVGAVGSGTLGGLAA
                     DALTRRAPPGGAVRRSLAILPPILVLLVGAFGALELNRTVRRAAADERSAAAIETVGR
                     RVEDFLRRHPGEPPLVRVSQSNWGDAAAVVLRVRKARLPVAIEPEWLVMFGEPFAPTG
                     RESRELILADPATFERARHAPGAVVIAETDAVSVVVVERR"
     gene            5521..6258
                     /locus_tag="PLT35_04290"
     CDS             5521..6258
                     /locus_tag="PLT35_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28477.1"
                     /translation="MLRSRSAACVVLALLLFVVLASTAGCGGHPQGPVVRKFFVASRM
                     GDATTLANLALTAFDARTEGEVVNLSIVSETPEQVAPLQLKALAEAFRAAQEAERQFT
                     DKWDAYRQVNREAIRKVHAAVEKGLTLKGPDAAMQEELSKWEAEGAQLTKNVSEAREA
                     ISRERPVVELSMRNPLKPIDATQYDGVLATKEMTVSANMKMPDGQSAKKTLVVTLKQA
                     RMKGPQGDIAGNWIVTNVASAPGGARP"
     gene            6722..>7916
                     /locus_tag="PLT35_04295"
     CDS             6722..>7916
                     /locus_tag="PLT35_04295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOG28478.1"
                     /translation="MGDEGRLFVAVDLGAGSGRVMLAGFRPQELMLEEVHRFHYPPRT
                     EGGHLRWDIGRIFAEIKAGLRKAGGRAQELGRPIRSVGIDCWGVDYGLVDAAGRLLED
                     PVCYRDSRTDGMMEAAFERVPKAEIFARTGIQFMPFNTLFQVLAHLREGLPAEAARLL
                     LVPDLLGFMLSGRQVTEYSIGTTTQMFEARAGTWDAGLLGRLGVPTRLLCEVVPSGTP
                     LGALLPEVAAETGLHGVQVVVPAAHDTGSAVAGAPLQPGWAYISSGTWSLAGVELPGV
                     LIDGEVARHNFTNEGGVYGTTRFLKNVMGLWILESCRREWREQGLPVDYDGLLRAAAA
                     IEGEAGLVYPDDPRLLNPPSMLAALAEQLRETGQEVPSAPPAMVKVILDSLACRYASV
                     FTTIEA"
BASE COUNT         1158 a         2801 c         2942 g         1015 t
ORIGIN      
        1 ctcccatgca caacgatcgt ctgtccaggc gtgagtggct gcgggcggcg ggcggaggag
       61 ccgttctgag cgccatccct ttccgggccc gcccggcatc ggcccaggcg gggggcaggc
      121 cgaactccaa ggtggcaggc gtccagctgg gcgtcaccac ctacagctac ctgtcgatgc
      181 ctcacaaggt cgatgacgtg attcagtacc tgctgtatgc gggcgtcaac tccatcgagc
      241 ttcggtcggt ggcggaagag gacctgggca ttccgcaggc cgcgggcccg gccggcggcg
      301 gcgcgccgct gaccgagcag gaaaaggccg agcgggccag ggccgccgag tcgctccgga
      361 acgcccagcg ggcgtggcgc ctgtcgctgc cgatggagcg gtacgcccgg atgcgccgga
      421 agttcaacga cgccggcatc gacgtccaca tcgccaagtt cgcgccgtcc tcgtggtcgg
      481 acgaggagat cgactacgcg tacacggcgg cgaagacgct cgggtcgatt ggcatcaccg
      541 acgagctcag cgagcaggcc tgccagcggc tcggccggtt cgccgaaaag caccggagcc
      601 tggcgatgtt ccacacccac ggacaggtgg cggaccccgg gttctccttc gacaagtacc
      661 tcgcgtactc gccggccaac atgctcaacc tggatgccgg ccactactac ggggccaccg
      721 ggctgcaccc gggcgacgtc atcgtcaagt accacgagcg gatgcgcagc atccacatca
      781 aggacaagac agggcccagg cacgcgacgc cgaacaccaa caagcccttc ggccagggcg
      841 agacgccgat cgccgacatt ctccttctgc tcaagaagga gaagtggccg atcgaagtcg
      901 acgtcgaact ggagtacccg atcccggacg gctccgatgc ggccagggaa acgaagaaat
      961 gcatcgacta cctgaggaac atcctcgagt agcggcagct gcgtgctcgg acgcgcagat
     1021 cgccggcgcg ccgggggagg ccgccgcctt caccgccacc cgggagctgc cggcccgccc
     1081 gtgacgcggc ggtcgcgggc agggcgcccg gatctcgagt ccaaggctca cagcggccgc
     1141 tgcggccgcg gccggaaccg gcggcgcacc agcagggccg ccgcgtgcca gccgtcacgc
     1201 cagcggatct tcttgccggc tcgccgtgaa cgcgacagag acgtcaccgg cagctcgagg
     1261 atccggacgc cgctccgcag caggttgacg gtgatctcgg gctcgatgtc gaaccggtcg
     1321 gcggtgagcc tgagcccgcg cgcgatatcg ccgcgcatga tcttgtagca cgtctccatg
     1381 tcggtcagcc gcgcgccgta cagcaggttg gtcagcatcg tcaggccggc gttgccgagc
     1441 ctcgacatga gcggcagacg cgaggagccg ccccggaagc gcgagccgta gacgacgtcc
     1501 gcgcctccct cgaggatggg cgcgacgagg gccggcagct gggcgggatc cagctcgagg
     1561 tccgcgtcct ggatggcgac gatggccccg gccgaccggg cgaagccggt gcgcagcgcc
     1621 gcgcccttgc cccggttcga ttcgaggtgc acggccgtga tgagaggcga gcgggcggcg
     1681 gcggcatcga gggcctggcg cgtgccgtcg tgcgacccgt cgttcaccgc gataatctcg
     1741 atcggcaccg ggaacgggac gccgagcagc cgatcgagca cggctccgat cgtggcttcc
     1801 tcgttgtaga ccggcacgat gatggagagg agcggcttca cgcgcgtcga ttctatcgga
     1861 tgggcggcga atccgcccgc ggcgcggcgg cgcaggccga cgggccgcgc cggcgcgcga
     1921 tggcggccgt cccgaacgat tgcatgacgt ccagctcggc ccggacgtcc cgaggcagtg
     1981 cgggcagatc taccagggcg tagggcagcc cgttcggagt cagccagcgc acgatcgcgt
     2041 cggccggttc gtaggtggaa ttgtcggcgg atcgcgccga cgcgcgggga ttccccagca
     2101 cggccaggtg cagccagtag gcggtctcgt cgtcgccgac gaggtactcg acgcaagagg
     2161 gcggcagatg ggatcgcgcc cagcgaccgg cctcgtacag cggcagggac acggccggcg
     2221 ggcgcggatg gaggagcctc ggcttcgcga tcaggggcct ggccgcgagg gccagcgctg
     2281 ctgccacgac cgcgagcgcg acggcgttgc cccaggcgcg ggacacgcgg acggtccggt
     2341 ccagccaggc ccaggcctcg cccgccgccg cgaccgcgca ggccgccatc ggccacagca
     2401 gcagatagcc catcttgagg gccatgtagg gctgtggcgc cccggcccgc cgtgccacca
     2461 ggcagagagc cgcggcctgg atgaggactg ccagcgcgaa gccggcggtc gcgcgtcccc
     2521 gctcccgggc agcggccacc aggaggcccg tggcggacag cacgaccagc ggcctgccgt
     2581 aggccgcgat cgacggccac ggcgccaggc cgcccgtgcc cgcgagccgc agccagccca
     2641 ggcggccgat ggcgaaggcc gcggcgacca gggcgagcgg cgcgaacgcc accgccaggt
     2701 cgcgcacgcg gcgcgcgaac ggccgccggc cgcgcgacgc gatcaagacg ccggcggcga
     2761 gggccggcgg cccgacgtac accggccacg cgagaaaagc cgccgcgccc gccgcgccga
     2821 acacgccgag ggcgagatcg gcgcgcgagt cggcccaggc tacgagggcc caccacatcg
     2881 ccacggccgg cagctcggcg acgacctgcg cgagaaagcc gaattcggtg aagccgccga
     2941 ggaagtagcg cggcgattcg agcagcagca ccggcccgag cgccgccagc agccgcggcg
     3001 cccgcggagg cagcaggcgc aggcatacgg caaccaggaa cacggccttc agcgcgaccg
     3061 tccacgcctg gactccgtgc atcgcgcgca acccgtcggt gccggaccag gcgcctgcca
     3121 gcgcaaccag gatgtggctg cccggggtgt agtgcgccat ctcacccagg gactgctcga
     3181 gcgccggatc gtgcaccagc cgccagtgcg tctcgatgtg gcggaccagc atcaggtggt
     3241 gcgtcagatc ggggcccgtg ccgagcggca gcagcgacgg agcggagagc cagagcagcc
     3301 aggcaagcag cagcgccccg gccgccgcgc cgagcatcag gccgacgcca tccggcttcg
     3361 cacgccggaa gaggagcgcg ccgcagaaga ggaacgccat ggccgccagc gccgccatgg
     3421 gcaacggcgt gaacgcgacg cccgcaaggt cggccgcgta cgcggcaagc ggcgtcagga
     3481 ccgctggcgc cagcagggac acggccgccg cctccgtcgt ggtcagccag ggagttccgg
     3541 tcggaggcgg cacggcgtcg agtataccgt caggcccgca acctccgcgc tcgtccggcc
     3601 cgggccggcg tatgcgagaa tggcccgcgc agcgctcgca ccaggatgga acagctggct
     3661 ccgcccacgg ctgacgcctc gcgcggcggc ttcccgcgcc cgcgcccgcc gcggcggtcg
     3721 ggcatccaac tcgtcgtcct gtttgccgcc gtcatggccg ccgtgtgcgt ggccgtcatc
     3781 gtggcgacgg ctccggcgtc ctggccgcga tcggacctgg ccgtcatcga aatcgacgtg
     3841 ctccacgcgc tgagcacgtt ctggaagctg gggccgtact cccgcttcgg ctggcgccac
     3901 ccggggccgc tgcacttcta cctgctcgcg ccgctctact ggctgtcggg cgagaagtcc
     3961 gcggcgctgc ccgccggcgc gttcctcctc aacctcggga gcgtggggtt cgtcgcctgg
     4021 gcggcaaggc gccacctccg gccgggaagc gccgtagggg tcaccctcgc gctggccgcg
     4081 ctcgccacca gggcggacgg attgctgacc agcgtctgga atccgcacgc agtggtcctg
     4141 ccgctggcgg ccacgatgac ggccgcggcg ctggcgtgcg cggatcacgc cggcttcctc
     4201 gtggcggcag tgctcggcgc atcgttgatc gcgcaaacgc acgtcggcct cgtgccggtc
     4261 gccctggcgg cagtcgcggc gccggccggc gcgatggcgg cgaacctggt gcgcgacggt
     4321 tgcgaagccc gtccgagcac gcgcgccgcg ctgcgggccc tggcggtttc catcgcgctc
     4381 ggcctgctcc tctggacgcc cccattgctc gagcagctgg agggaaggcc ggggaacctc
     4441 gcgcaggtcg cggcgtactt cacgtcggcg gagcggaccg gacagccggt agccgctgcg
     4501 gcgagggccg tcgggtacga gttctcctgg atctggcggc cgcggcccgc gctgccggac
     4561 gggaagccgc tgcggcgtca gccggcgaga gcgtgggtgg tgtggagtgg gagccagctg
     4621 gcggcgatcg cggcgctgct ggtcgcctgg cggcatggcc gccgggatcc ccgctggtgg
     4681 ctgggcgtgg tcaccctgct tgccgcctcc atgggacttg cgtcggccac gagggtcgat
     4741 ccgccggcgc ccgatcacgc gctgttctgg ctcgccgccg tcggcgccgt cggttccggc
     4801 acgcttggcg gcctcgcagc cgacgcgctg acgcgccgag cgccgcccgg cggcgcggtg
     4861 agacggtcgc tcgcgatcct gccgccgatc ctcgtgctcc tggtcggggc cttcggcgcg
     4921 ctggagctga accgcaccgt gcggcgcgcg gcggccgacg aacgcagcgc ggcggcaatt
     4981 gagacggtcg gccgccgggt cgaggacttc ctgcgccgcc atccgggcga gccgccgctt
     5041 gttcgcgtct ctcagtcgaa ctggggagac gcagccgccg tcgtgctccg cgtccgcaag
     5101 gcgaggctgc cagtcgccat cgagcccgag tggctggtga tgttcggtga gccgttcgct
     5161 cccaccggcc gggaatcccg cgagctgatc ctggcggatc cggccacctt cgagcgcgcg
     5221 cgccacgcac cgggcgcggt ggtcatcgct gaaaccgacg ccgtgtcggt cgtcgtcgtc
     5281 gagcgccgct agaggcgggc gcgcccgccc ggtcaccgcc gcggcgaagc cgtcgacgcg
     5341 atggccgggc gcggccgaac cgggccccgc agtgctagaa tcctcccgac agcccgcgga
     5401 gtacatggct cggaccgctt cccaggcgcg tccgatcgtc ggcgaaggcg ctgcggctcg
     5461 gggttcggag caggccggca cctgcaccgg gccccggagc cgaggaaagg aggcccgctc
     5521 atgctccgat cgcgatccgc ggcgtgcgtc gttctcgccc tcctcctgtt cgtcgttctc
     5581 gcctccaccg ccgggtgcgg tggacatccg cagggccccg tcgtgcggaa gttcttcgtg
     5641 gcttcacgca tgggggacgc gacgacgctg gcgaacctcg ccttgacggc cttcgatgcc
     5701 cgcaccgagg gcgaggtcgt gaacctgagc atcgtgtcgg agacgcccga gcaggtggcc
     5761 cccctgcagc tgaaggcgct cgcagaagcc ttccgggccg cccaagaggc cgagcggcag
     5821 ttcaccgaca agtgggacgc gtaccggcag gtcaaccgcg aggcgatcag aaaggtgcac
     5881 gcggccgtcg agaagggcct cacgctcaaa ggcccggacg ccgcgatgca ggaggagctg
     5941 agcaagtggg aagctgaagg ggcgcagctc accaagaacg tcagcgaggc cagggaggcc
     6001 atctcgcgcg agcggccggt ggtcgagctg agcatgagaa accccctcaa acccatcgac
     6061 gcgacccaat acgacggcgt gctggccacc aaggagatga ccgtctccgc gaacatgaag
     6121 atgccggacg gccagtcggc caagaagacg ctggtcgtca cgctgaagca ggcgcggatg
     6181 aagggccccc agggcgacat tgccgggaac tggatcgtca cgaacgtcgc cagcgcccca
     6241 ggaggcgcca ggccgtagtc ggaggccagg gcggcagtcc gggctgccgc cgggccggcc
     6301 gctccggccg gcctgtgcgc ccggcgagat cgcagatcgc tccgggtcga aggcctccga
     6361 ggcgcctggc atcccgcccg cgccctgttg cgcgcacctg gtcgatgtcc gcgatctgcc
     6421 ggcgcgcctg gaggagccgg tctgcgggac gcgcgacgag cgcgccttcg gcgtcgtggc
     6481 gccgtctgtg gcggccgacg cgcgcgggca tcagggccgt cgtcgaatcc gtcctcgagc
     6541 gcccggcatg acggctgggt cggccgttgt gtgttgacca gacgagcgga gtccgccatg
     6601 atcccccgca accgcagcgg ctgaggccgg aggccgcccg ggccacgcgg tcgagcccct
     6661 gatccggcgt cgggcttccc cggtgctccc agggggccgc ggcccgccgg aggggttgtc
     6721 aatgggcgac gaagggcggc tgtttgttgc ggtggatctg ggggcgggaa gcgggcgggt
     6781 catgctcgcc ggcttccggc cgcaggagct gatgctcgag gaggtccacc gcttccacta
     6841 ccccccgcgc accgaaggcg ggcacctgcg gtgggacatc gggcgcatct tcgccgagat
     6901 caaggccggg cttcgcaagg ccggaggccg cgcgcaggaa ctcggccggc cgatccgcag
     6961 cgtcggcatc gactgctggg gcgtggacta cggcctcgtc gatgccgccg ggcggctgct
     7021 cgaagacccg gtctgttacc gcgactcgcg caccgacggc atgatggagg cggcgttcga
     7081 gcgggtgccg aaggcggaga tcttcgcgcg gacgggcatc cagttcatgc ccttcaacac
     7141 gctgttccag gtgctcgccc acctgcgcga gggcctgccg gccgaggccg cgcgcctgct
     7201 cctggtgccc gacctgctcg gcttcatgct ctccggccgc caggtgacgg agtactccat
     7261 cggcacgacg acgcagatgt tcgaggcgag ggcgggcacg tgggatgccg ggctgctcgg
     7321 ccgcctgggc gttccgaccc gcctcctgtg cgaggtggtg ccgtcaggca cgccgctcgg
     7381 cgcgctgctg ccggaggtgg ccgccgagac cgggctgcac ggcgtgcagg tggtcgttcc
     7441 ggccgcgcac gacacgggca gcgcggtggc cggcgcgccc ctgcagccgg gctgggccta
     7501 catctcgtcg ggcacgtggt cgcttgccgg cgtggagctc ccgggcgtgc tgatcgacgg
     7561 cgaggtcgcg cggcacaact tcacgaacga aggcggcgtc tacggcacga cgcggttcct
     7621 caagaacgtc atgggcctgt ggatcctcga gtcgtgccgg agggaatggc gcgagcaggg
     7681 gctgccggtc gactacgacg gcctgctccg ggcggccgcc gcgatcgagg gcgaggcggg
     7741 cctggtgtat ccggacgatc cgcggcttct gaaccccccg agcatgctgg ccgcgctcgc
     7801 agagcagctg cgcgagacgg gacaggaggt gccgtcggcg ccgccggcga tggtcaaggt
     7861 gattctggac tcgctggcct gccgctacgc ctccgtgttc acgacgatcg aggcgc
//
LOCUS       DAOSIF010000081         7814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8607_length_7814_cov_4.496456, whole genome shotgun sequence.
ACCESSION   DAOSIF010000081 DAOSIF010000000
VERSION     DAOSIF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8607_length_7814_cov_4.496456"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PLT35_04300"
     CDS             <1..1191
                     /locus_tag="PLT35_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28479.1"
                     /translation="EPIFKGLGFAKALAAKGHRVEVLTGFPNYPGGRILPGYRVRLRQ
                     REQMQGIPVTRVALYPSHDASAIRRSANYLSFAASAATVGACSVTGPDVVYAYHPPAT
                     IALPAMVIGAARGAPFVLDVQDLWPDTLLATGMASRPWLLKAAGAFCDLAYCRAKRIS
                     VISNGFKRALVARGVPEDKIEVIYNWGREETSAPGGPDAPILREAAIEGRFSVVFAGT
                     MGRAQALSSVLEAAALVARRAPQVRFVFVGGGIEAAELQARAAEMALPNAAFLPLRPA
                     AEVAPLLEAANVLLVHLRDDPLFSITIPSKTQAYMQAGRPVLMAVRGDAADLVREAGA
                     GVCVPPEDPEALAAAVCDLAAAPPEALDAMGARGRAYYQAHLSFAAGVARVDELLRRV
                     AAGG"
     gene            1234..1839
                     /locus_tag="PLT35_04305"
     CDS             1234..1839
                     /locus_tag="PLT35_04305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG28480.1"
                     /translation="MTFYRRYGKRACDLAASVAALIVFLPLMAALALAVRLVMGSPVL
                     FRQQRPGLRARPFTLLKFRTMTEARDEAGELLPDERRLTRLGAFLRRWSLDEIPELWN
                     VLRGDMSFVGPRPLLMRYLPRYRPDQARRHDVRPGISGWAQVNGRDLVTWTQRFAMDV
                     WYVEHVSFSLDVKIAALTVWKALRREGVLDDGRATKDEFLG"
     gene            1843..2835
                     /locus_tag="PLT35_04310"
     CDS             1843..2835
                     /locus_tag="PLT35_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG28481.1"
                     /translation="MLNVLFTSAGRRVELLRAFRKAYATLGLDGRVVAVDMDPLAPAL
                     GAADRAFIVPRLSAPDYIDALSEVCRRERVKVVFPLIDPDIPVLAANRAALEAAGARP
                     AVVSAEAAAIAADKWRCSQFFAGLGLETPRSWLVADLARGGFDYPLFVKPRRGSAAVN
                     AFAARNPRELEFFTQYVPDAVVQEFLPGPEITSDVVCDLDGGVLAVVSRQRIEVRWGE
                     VAKGVTVSDPRITEACVAIARALPAVGPITVQCMMKDGAPRFTEINARLGGGAPLGIA
                     AGADWPCWLLARCAGVQVEVPPLGSYRTGTYLTRFDESVFMTEAERDEIARRRL"
     gene            2807..3541
                     /locus_tag="PLT35_04315"
     CDS             2807..3541
                     /locus_tag="PLT35_04315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG28482.1"
                     /translation="MTRSRAVVFDLDDTLYPERDFVLGGFDAAAAWGETHLGIPKDEG
                     YRALLRMYEDGVRGDTFDRWLGARGIEPGPIVPQLVDAYRRHAPRIAPFPGVPAMLGR
                     LRAGHRVGLVSDGFLAVQRRKLDALGIGRLFDAVVFSDEWGRDGWKPSPAPFLAALER
                     LGVAADSAVYVGDNPLKDFLGARLAGMRTVQVRRPGGEYCRAVAPTKAHEADRVLRDL
                     DELEHLLASWPGADGPADGERGERRA"
     gene            3573..4697
                     /locus_tag="PLT35_04320"
     CDS             3573..4697
                     /locus_tag="PLT35_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG28483.1"
                     /translation="MDRIYLSPPHVFGDEQALVADAFASNWIAPLGPHVDAFEREFAA
                     CVAVPHAVALSSGTAALHLAMVLLGVGPGDTVICPSLTFSASANPIRYQGASPVFVDC
                     DRTWTMDAGLLEDALKQAAAAGRLPRAVIAVDLYGQCADYGAIGPLCERYGLPLVEDA
                     AEALGATHGGRPAGGLGRIGVFSFNGNKIVTTSGGGMLVAHEKPLAEAARFLATQARD
                     PAPHYEHSHIGYNYRMSNVLAAIGRGQLTSLAERVAIRRRIFDRYVAGLGDLPGITFM
                     PEAPYGRCTRWLTCLVIDAREFGADREAVRLALEAENIEARPVWKPMHMQPVFAGAAM
                     FGGAVSERLFRDGLCLPSGSSLREQDQARVISAIRRTSRT"
     gene            4723..7038
                     /locus_tag="PLT35_04325"
     CDS             4723..7038
                     /locus_tag="PLT35_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOG28484.1"
                     /translation="MSEPSTPGPAWPSERTSETADTEPRAPRTYGGSSPYAQPGGGGG
                     VGPGEDAEFRSTHLVDTVRTIYKHRWIALTTFLVLFLGSAIRTFTTTPTYEGQVQLLL
                     DPSNPNVMKFQEVNQGSYLYEQYFQQTQVNILRSRGLARRTIEALKLWDAPELGGGER
                     TGSVLARIGGLFSGRKKAAAPASGETAKQSLTIDAFLKSLTVAPIRNSSLVNVRFASP
                     NPELAASAANTLARQYIEQNLEYKFTSTKDASDWLASQLAVERKKLEASEQALQRYRE
                     AGDAVALEDRQNIVVQRLADLNAAYTQARTERFEKEALYNQLRSLQHEPAALDTFPAI
                     LANAFIQQLKAQLADLQRQKAQMAERLGEKHPDMIKLNSAIEATEARLAGEVGKIVQS
                     VRNEFLSAESKERSLAAELESQKAGALALNRKGIEYGVLRREAESNKQMYEALLQRAR
                     ETDVSGEMKASNIRIVDQAEVPRRPVRPRKAMNLVLGFLAGLASGLGLALFVEYLDNR
                     VKSPDEIKHHLGLSFLGLVPAARQEDVAAGRSPLISETVAQNFAEAVREIRTSVMFSS
                     ADDGPRSVLVTSTQPAEGKSVIAANLAVSLANAGLRVLLLDADMRKPRLHDLMKTKQS
                     PGLSSLLVGQAKANEVVYKTGTPNLWIMPAGPNPPNPAELLGSTRFLNLVTSLGEHFD
                     WTVIDSPPVMAVTDPSVVAHRTTGVVFVIGSEQVDRRKALTAVQKLQASKGKILGAVL
                     NRVNVERNPYYYSHYYSHEYAGYYSSDKKTA"
     gene            7070..>7814
                     /locus_tag="PLT35_04330"
     CDS             7070..>7814
                     /locus_tag="PLT35_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28485.1"
                     /translation="MTHAAVPPPLPSLVLRFRRPLAVVIYIGLAWLSNVLAFQVRFDG
                     QVPPDARRSCLAMLPWLLALRAGGFMAFGQFRGLWRYASLYDLRNIVLAVLSSSAAFY
                     GLVRWVVAQPLYPRSVYLIDALLLITLLGSLRIARRAWMENRPQAHAKRVLVVGAGDA
                     GEMIVRDMRHNRRHEYVPVGFVDDDPGKRGEQIHGVPVLGGRADIARIVEKVRPADVL
                     IAMPSAPASVRRDVVRALQAYHVNITTLPD"
BASE COUNT         1136 a         2710 c         2821 g         1147 t
ORIGIN      
        1 gagcccatct tcaagggcct gggcttcgcg aaggcgcttg ccgcgaaggg ccaccgggtg
       61 gaggtcttga ccgggttccc gaactacccg ggagggcgga tccttccggg ctaccgcgtg
      121 aggctccggc agcgtgagca gatgcagggc atcccggtga cgcgggtggc gctctacccg
      181 agccacgacg cgtcggccat caggcggtct gccaactacc tgagcttcgc ggcgtcggcc
      241 gcgaccgtcg gagcgtgctc cgtcaccggg ccggacgtcg tctacgccta ccatccgccc
      301 gcaacgattg cgctgccggc gatggtgatc ggcgcggcga ggggcgcccc cttcgtcctc
      361 gacgtgcagg atctctggcc cgacacgctc ctggccacgg gcatggcgtc gaggccgtgg
      421 ctgctgaagg cggcaggggc gttttgcgat ctcgcgtact gccgcgcgaa gcggatttcg
      481 gtcatctcca acggcttcaa gcgcgcgctg gtagcccggg gcgttccgga agacaagatc
      541 gaggtgatct acaactgggg ccgcgaggag acgtcggcgc caggagggcc ggacgcaccc
      601 atcctgaggg aagccgccat cgagggccgc ttcagcgtgg tgttcgcggg cacgatgggc
      661 cgcgcccagg ccctgtcgtc ggtgctggag gccgccgcgc tggtggcccg aagggccccg
      721 caggtccgtt tcgtcttcgt cggcgggggc atcgaagccg ccgagctgca ggcccgcgcg
      781 gccgagatgg ccctgcccaa cgccgccttc cttcccctgc ggccggcggc ggaggtggcg
      841 ccgctgctcg aggcggcgaa cgtgctcctg gtgcacctgc gagacgaccc gctgttctcg
      901 atcacgattc cttcgaagac gcaggcgtac atgcaggcgg gccgcccggt cctgatggct
      961 gtccggggcg acgccgccga cctggtccgg gaggccggcg cgggcgtgtg cgtgccgccc
     1021 gaagacccgg aagcgctggc agcggcggtg tgcgatctcg ccgcggcgcc gccggaggcg
     1081 ctcgacgcga tgggggcgcg gggccgggcc tactaccagg cgcacctctc gttcgccgcc
     1141 ggtgtggccc gcgtcgatga gctgctccgg cgcgtggccg ccggcggctg acgccgacgg
     1201 aggccgcttc ttcacaccct gcgggccgac cgcgtgacgt tctaccgccg ttacggcaag
     1261 cgcgcctgcg acctcgccgc gagcgtggcg gcgctcatcg tcttcctccc gctgatggcc
     1321 gcccttgcgc tggcggtccg cctcgtcatg ggatctccgg tgctcttccg tcagcagcgc
     1381 cccggcctgc gcgcgcgtcc gttcacgctg ctcaagttcc ggaccatgac cgaggcgcgg
     1441 gatgaggcgg gggagctgct gcccgacgag cggcgcctga cgcggctggg agcgttcctg
     1501 cgccgatgga gcctcgacga gatccccgag ctctggaacg tcctgcgcgg cgacatgagc
     1561 ttcgtcggcc cgcggcccct cctcatgcgg tacctgccgc gctaccggcc ggaccaggcg
     1621 cgccggcacg acgtcaggcc cgggatctcg ggatgggccc aggtcaacgg gcgcgacctg
     1681 gtcacgtgga cacagcggtt cgcgatggac gtgtggtacg tggagcacgt gtcgttttct
     1741 ctcgacgtga agattgccgc cctcaccgtc tggaaggcgc tgcgccggga aggcgtcctg
     1801 gacgacggcc gggcgacgaa ggacgagttc ctcggctgag ccgtgctgaa cgtgctgttc
     1861 acgagcgccg gccgccgggt ggagctgctg cgggcgttcc gcaaggccta cgccaccctc
     1921 ggcctcgacg gccgcgtcgt ggccgtggac atggatccgc tcgcccccgc gctcggcgcg
     1981 gccgatcgcg ccttcatcgt gccgaggctc agcgcgcccg actacatcga cgcgctgtcg
     2041 gaagtctgcc gccgcgagcg cgtgaaggtc gtctttccgc tgatcgatcc ggacatcccg
     2101 gtccttgcag ccaaccgggc ggcgctggag gccgccggcg cgaggccggc ggtcgtcagc
     2161 gccgaggccg ccgccatcgc ggccgacaag tggcggtgct cgcagttctt cgccggcctc
     2221 ggactcgaga cgccgcgctc ctggctggtc gcggacctgg cgcggggggg cttcgactac
     2281 ccgctgttcg tgaagccgcg ccggggaagc gcggcggtga acgcgttcgc cgccaggaac
     2341 ccgcgcgagc tggagttctt cacgcagtac gtgcccgacg ctgtcgtaca ggagttcctg
     2401 cccggcccgg agatcacgag cgacgtcgtg tgcgatctcg acggcggcgt gctggcagtc
     2461 gtgtcgcggc agcgaatcga ggtgcggtgg ggcgaggtgg cgaagggcgt gacggtgagc
     2521 gatccgcgca tcaccgaggc gtgcgtggcg attgcgcgcg cgctcccggc cgtcggcccg
     2581 atcaccgtgc agtgcatgat gaaggacgga gcgccgcgct tcaccgagat caacgcgcgg
     2641 ctcggcggcg gggcgccgct cggaatcgcc gccggcgccg actggccctg ctggctgctc
     2701 gcccggtgcg ccggcgttca ggtcgaggtt ccgccgctcg ggagctaccg gaccgggacc
     2761 tacctgacgc gcttcgacga gtccgtgttc atgaccgagg ccgagcgtga cgagatcgcg
     2821 cgccgtcgtc tttgatctcg acgacacgct ctatcccgag cgcgacttcg tgctcggcgg
     2881 gttcgacgca gccgcggcgt ggggcgagac gcacctcggc atcccgaagg acgaggggta
     2941 ccgcgctctc ctccgcatgt acgaagacgg cgtgcggggc gacacgttcg acagatggct
     3001 cggcgcgcgg ggcatcgagc ccggcccgat cgtgccgcaa ctggtggacg cgtaccgcag
     3061 gcacgcgcct cgcatcgcgc cgtttccggg cgtgcctgcg atgctcggca ggctgcgcgc
     3121 cggccaccgg gtggggctgg tgagcgacgg gttcctcgcg gtgcagcggc gcaaactgga
     3181 tgcgctcggg atcggcaggc tgttcgacgc cgtcgtgttc tccgacgaat ggggccgcga
     3241 cgggtggaag ccgagcccgg cgccgttcct ggccgcgctc gaacggctcg gtgtggcggc
     3301 cgattcggcg gtctacgtgg gagacaaccc gctgaaggac ttcctgggcg ccaggctggc
     3361 cggcatgcgc acggtccagg tgcgtcgtcc tggcggcgag tactgccgcg cggttgcgcc
     3421 aacgaaggca cacgaggcgg accgcgtgct ccgcgatctg gacgagctcg agcacctgct
     3481 cgcgtcctgg cccggcgctg acggcccggc ggatggcgag cgcggagagc gccgggcata
     3541 gcgcacggcc gctggcgcga gaggggagcc gcgtggatcg gatctacttg tcgccgcccc
     3601 acgtcttcgg agacgagcag gcgctggtcg ccgacgcctt tgcgtcgaac tggattgcgc
     3661 cgctgggccc gcacgtggac gccttcgagc gcgagttcgc cgcctgcgtg gcggtgccgc
     3721 acgccgtggc gctgtcgtcg ggcacggccg ccttgcacct ggccatggtg ctcctcggcg
     3781 tcgggcccgg cgacacggtg atctgcccgt ccctcacgtt cagcgcgtcg gcgaacccga
     3841 tccgctacca gggcgcctcg cccgtcttcg tggactgcga ccgcacgtgg accatggacg
     3901 cggggctgct cgaggacgcg ctgaaacagg ccgccgccgc cgggcggctg ccgagggccg
     3961 tgattgccgt ggacctctac ggccagtgcg cggactacgg cgccatcggc ccgctgtgcg
     4021 agcggtacgg cctgcccctc gtggaggacg cggccgaagc gctcggcgcc acgcacggcg
     4081 gccgtcccgc cggcgggctc ggccgcatcg gcgtcttctc gttcaacggc aacaagatcg
     4141 tcaccacctc cggcggcggc atgctcgtgg cgcacgagaa gccgctggcc gaggccgcgc
     4201 ggttcctcgc cacccaggcg cgcgacccgg cgccgcacta cgagcattcg cacatcggct
     4261 acaactaccg catgagcaac gtgctcgcgg cgatcggccg cgggcagctg acgtcgctgg
     4321 ccgagcgcgt cgcgatccgc cggcggatct tcgatcggta cgtcgccggg cttggagacc
     4381 tgccgggcat cacgttcatg cccgaagcgc cgtacggccg gtgcacgcga tggctcacgt
     4441 gcctggtcat cgatgcgcgg gagttcggcg cggatcgcga ggcggtgcgc ctggccctgg
     4501 aagcggagaa tatcgaggcg cggccggtct ggaaacccat gcacatgcag cccgtcttcg
     4561 cgggagcggc gatgttcggc ggcgccgtgt cggagcgcct gttccgcgac ggcctctgcc
     4621 tgccgagcgg atcgagcctg cgggagcagg accaggcgcg ggtgatctcc gcgattcggc
     4681 gaacgagccg gacgtagtac gattcctgga gagaaggctc cgatgagcga accatcgacg
     4741 ccgggcccgg cctggccgtc cgaacgcacc tccgaaacgg cggacaccga gccgagggcc
     4801 ccgcgcacgt acgggggatc gagtccgtac gcccagccgg gcggcggagg cggggtcggc
     4861 ccaggagagg acgccgagtt ccgctcgacg cacttggtgg acaccgtccg gacgatctac
     4921 aagcaccgct ggatcgccct gacgacgttc ctggtgctct tcctcggatc ggcgatccgg
     4981 accttcacca ccacgccgac ctacgaaggg caggtccagc tcctcctcga tccatcgaac
     5041 ccgaacgtga tgaagttcca ggaggtcaac caggggagct acctgtacga gcagtacttc
     5101 caacagacgc aggtgaacat cctgaggagc cgcggcctgg cacgccgcac gatagaggcg
     5161 ctgaagctgt gggacgcgcc cgagctcggc ggcggcgaga ggacaggctc cgtcctggcg
     5221 cggatcgggg gtctgttctc ggggcggaag aaggcggcag cccccgcgtc cggggagacc
     5281 gccaagcagt cgctcacgat tgacgcgttc ctgaagtcgt tgaccgtcgc gcccatcagg
     5341 aacagcagcc tcgtgaacgt ccgcttcgcg tcgcccaacc ccgagctggc cgcgtcggca
     5401 gccaacacgc tggccaggca gtacatcgag cagaacctcg aatacaagtt cacgtcgacg
     5461 aaggatgcgt cggactggct ggcctcgcag ctggccgtcg agcgcaagaa gctcgaggcg
     5521 agcgagcagg cgctgcagag gtaccgcgag gcaggcgacg ccgtcgccct ggaggaccgc
     5581 cagaacatcg tcgtgcagcg cctggccgac ctcaacgccg cctacacgca ggcgcgcacg
     5641 gaacggttcg agaaggaggc gctctacaac cagcttcgga gcctgcagca tgaacctgcg
     5701 gccctcgaca cgttcccggc gatccttgcc aacgcgttca tccagcagct caaggcgcag
     5761 cttgccgacc tccagcgcca gaaggcccag atggccgagc gcctgggcga gaagcacccg
     5821 gacatgatca agctgaactc ggccatcgaa gccaccgagg cgaggctcgc cggcgaggtc
     5881 gggaagatcg tgcagtcggt gcgcaacgag tttctgtcgg cggagtcgaa ggagcgcagc
     5941 ctcgcggcgg agctggaatc gcagaaggcc ggggcgctgg cgctgaaccg gaagggcatc
     6001 gagtacggcg tgcttcggcg cgaagccgag agcaacaagc agatgtacga ggccctcctg
     6061 cagcgcgcgc gggaaacaga cgtttccggc gagatgaagg ccagcaacat ccgcatcgtg
     6121 gaccaggcgg aggtgccgcg caggcccgtc aggcccagga aggcgatgaa cctcgttctc
     6181 ggattcctcg ccggcctcgc gtcggggctc ggcctcgccc tgttcgtcga atacctcgac
     6241 aaccgcgtca agagcccgga cgagatcaag catcatctgg ggctcagctt cctgggcctc
     6301 gtgccggccg cccgccagga ggacgtggcg gcggggcgct cgccgctcat cagcgagacc
     6361 gtggcccaga acttcgcgga ggcggtccgg gagatccgca cgagcgtcat gttctcgtcg
     6421 gccgacgacg ggccgcggtc ggtgctcgtc acgagcacgc agccggcgga gggcaagtcg
     6481 gtgatcgccg cgaacctcgc cgtgtcgctg gcgaacgcgg gcctccgggt gctgctgctg
     6541 gacgcggaca tgcgcaagcc gcggctgcac gacctgatga aaacgaagca gagccctggc
     6601 ctgtcgagcc tgctcgtcgg ccaggcgaag gcgaacgagg tcgtgtacaa gaccggcacg
     6661 ccgaacctct ggatcatgcc ggccgggccg aacccgccca acccggcgga gctgctcggc
     6721 tcgacgcgct tcctgaacct cgtgaccagc ctcggcgagc acttcgactg gaccgtgatc
     6781 gactcgccgc ccgtgatggc cgtcaccgat ccctccgtgg tggcgcaccg gacgacaggc
     6841 gtagtgttcg tcatcggcag cgagcaggtc gatcggcgca aggcgctcac cgcggtgcag
     6901 aagctgcagg cgtcgaaggg gaagatcctc ggggccgtgc tgaaccgcgt gaacgtcgag
     6961 cgcaacccgt actactactc gcactactac tcccacgaat acgccggcta ctactcgagc
     7021 gacaagaaga ccgcgtaaac acccgggccc ccgctcgttt ccagaggccg tgacgcacgc
     7081 cgccgtccct ccgccgctcc cgagcctggt gctccgcttc cgccggcccc tcgccgtcgt
     7141 catctacatc ggcctggcgt ggctgtcgaa cgtcctggcg ttccaggtgc ggttcgacgg
     7201 gcaggtgccg cccgacgcgc ggcgctcctg cctggcgatg ctgccgtggc tcctggcgct
     7261 gcgggccggc gggttcatgg ccttcggcca gttccgcggc ttgtggcggt acgccagcct
     7321 ctacgacctg cgcaacatcg tcctggcggt gctgtcgagt tccgcggcct tctacggcct
     7381 cgtccgctgg gtcgtcgccc agccgctgta cccgcggtcg gtctacctca tcgacgccct
     7441 gctgctcatc acgctgctcg gctcgctccg catcgcgcgc cgggcctgga tggagaaccg
     7501 gccgcaggcg cacgcgaagc gtgtcctcgt cgtgggcgct ggcgatgcgg gcgagatgat
     7561 cgtccgcgac atgcggcaca accggcggca cgaatacgtg cccgtcgggt tcgtggacga
     7621 cgaccccggg aagcgcggcg agcagataca cggcgtgccc gtgctcggcg gacgggcgga
     7681 catcgcgcgc atcgtcgaga aggtgcgccc ggccgacgtg ctgatcgcga tgccgtcggc
     7741 gccggcgtcg gtgaggcggg acgtggtgcg ggcgctgcag gcctaccacg tgaacatcac
     7801 cacgctgccg gacc
//
LOCUS       DAOSIF010000082         7794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8633_length_7794_cov_3.507818, whole genome shotgun sequence.
ACCESSION   DAOSIF010000082 DAOSIF010000000
VERSION     DAOSIF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_7794_cov_3.507818"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLT35_04335"
     CDS             <1..570
                     /locus_tag="PLT35_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOG28486.1"
                     /translation="KPRGWVMVNGQRVPWETCQTFSGSWGYHRDEATWKSPAQLIELL
                     VDTVSKGGNLLMNVGPTARGTFDQRAKDRLAAFGRWTQANGRSIYGATQAPPEFKAPP
                     NTLLTYNPATKRLYVHVLAWPMGDIVLEGYAGKVAYAQLLHDASEVRLSERRATIWMD
                     EAGKTGDLVMRLPMLKPDVEIPVVELVLK"
     gene            924..2219
                     /gene="eno"
                     /locus_tag="PLT35_04340"
     CDS             924..2219
                     /gene="eno"
                     /locus_tag="PLT35_04340"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOG28487.1"
                     /translation="MSCAACSGRAIDTVRALEILDSRGNPTVSVTVTLENGVTASAAV
                     PSGASTGEHEAVELRDGDKSRYGGKGVLKAVRNVNEVIAPKVEGLDATRQREIDLLMK
                     DLDGTANKGVLGANAILGVSMAVARAAAASSDLPLYAYLGGPGATRLPVPMMNILNGG
                     AHANNSVDLQEFMVMPIGAPTFAEALRAGAETFHALKGILKQRGYATAVGDEGGFAPD
                     LKSNEEACEVILEAISAAGYKPGQDVSIALDPAASSFFEDGAYNLAKSRQGRKTHAEM
                     TAIYRDWIAKYPIVSIEDGLAENDWDGFKAHTAALGDRIQIVGDDLFVTNTAFVARGI
                     AEKAANAVLIKLNQIGTVSETIDTIAMCRKAGWGFVISHRSGETEDAFLADFAVAMGG
                     GQIKTGSACRSERMAKYNRLLEIEAELGAAAVFTNPFAR"
     gene            complement(2381..4099)
                     /locus_tag="PLT35_04345"
     CDS             complement(2381..4099)
                     /locus_tag="PLT35_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOG28488.1"
                     /translation="MKFLGSEIAYLISEREMRGNIRALLRYLLLLAAVITTHSVLFHL
                     IMTGLEGQRHSWVTGFYWTMVVMTTLGFGDITFTTDVGRAFSVVVLLSGVVLLLVLLP
                     FLFIRLFYAPWFEARVRLRAPRRVPAGTAGHVLIAEFDDVASGLAARLASERVPHFVI
                     EPDVVRAGQLVNDGVPVVAGRNDSRATYEQLGIERASLVLANCTDTVNTNITLTVREA
                     APRVPIVAIVEAEDSADILRLAGATHVLPLKHQLGEHLANRVEAGAAEAHVIGAFGDL
                     LIAELPARGTPFAGNSVRDTRLRERTGLNLVGLWERGRLLPAYPQTLIRPDSVVVVAG
                     TAGHIAALGRLLPASDAEPRPVIVIGAGTVGRAAALALKRKGLQVHVIERDEAALALL
                     AGTVDRAFQGDAADRALLDRAGIGSARSVLLTTNDDAMNIFLAVYCRRLQPEIRIVSR
                     ITHERNLEAIHRAGADFVLSYTSLGVDAVLAILRGHELVLLGEGVELFSIPVPASLAG
                     LPLRESQIGSRTGLSVVALKHGDGSSTPLDADAVLRADARLVMLGSLEQRRAFADAFE
                     RRPGQP"
     gene            4173..5660
                     /locus_tag="PLT35_04350"
     CDS             4173..5660
                     /locus_tag="PLT35_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOG28489.1"
                     /translation="MRRAVLALAFTGVAAAAAAQPYQPTWESLDARPTPAWFQDAKFG
                     IFIHWGVYSVPAYAPVLPGKLAYSEWYWNALTGGKGAKPSPIQAGTWEYHRQRYGADF
                     SYQEFAPQFRAELFDADHWAEVFRRSGAKYVVPTSKHHDGFAIWPSREASASWGRPWN
                     AAEIGPRRDLLGELTAAVRAKGLRMGFYYSLYEWYNPIWLYDKPRYVREHMFPQFKDV
                     VTRYQPSIIFSDGEWDLPSSEWKTPELLAWLFNESSVKDEVVVNDRWGRDCRHKHGGY
                     WTTEYTPGMAEASHPWEESRGMGFSYGYNRAERLDHYRTGRELVTILVDTVSRGGNLL
                     LDIGPDADGTIPVIMEQRLAEIGAWLDVNGEAIYGTRPFTAAKQWSAGEVPTIDYNKE
                     YDSDYDVARLVAPSAPGKAWIEAFFTQKGDAVYAILTRWPGRQFVWKNAAGLKPKSVT
                     LLGGRDSMRVRKSGTALIVDLPELPAELLGQPLWVLKLSRERASR"
     gene            complement(5652..6782)
                     /locus_tag="PLT35_04355"
     CDS             complement(5652..6782)
                     /locus_tag="PLT35_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG28490.1"
                     /translation="MSAAVFVVVVLAIWGSMHAVLLWHLLSIPAATELLPRWAIAAAV
                     AALGIAFPASRLLAATPLRALAPPLEVVGATWMGIIWLALTCLLVAELATGFGLWAAP
                     IRPALRGWALLAAAALSVAALVQGLRPPVVRRHDVTLAALPGARDGTVLVFVSDLHLG
                     TMTGDSWLRARIAQVGALDPDIVVIGGDVFEGDGERVRAMLPTLRQITAPLGVFAVTG
                     NHDEYGRGADGRSPLDDAAIRVLRDEWVQIVPGLVIAGVSDGGIRANRVPPAGQIARA
                     LEGRPPGAATVFVSHMPVGADVAASLGAGLMLSGHTHGGQIWPFGLIVRRVMPLFAGR
                     YDVAGMPVLVCRGTGTFGPRMRLWRRGEILCVTLRAAPIGSS"
     gene            complement(6861..>7794)
                     /locus_tag="PLT35_04360"
     CDS             complement(6861..>7794)
                     /locus_tag="PLT35_04360"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOG28491.1"
                     /translation="KKILYGLSFAIYSLSEYTLATGDPRGIEYAQRTFDLVERHCADP
                     ALGGYFEMFERDWTRCGPGAGGGDRKTLDVHMHLMEAFTTLFECSRRAEHRRALQENI
                     DLLVGRILHPQYATGIPQFWADWRVAPQIKFDIVWGWDRFAEGGAKPHADDNTSYGHN
                     VECAWLLLHALDVMGADRRPYEPVVRKQLDHAVRAGVDFEYGGVYVEGPHDGGPAYDT
                     EKEFWQQAETMIGMLDGCRLFGMEPYWQAYEAVHRFVMDRMVNREVGEWWPLLSRDGR
                     TRIWTHMSHSWKINYHSVRGAIQSIARLEELLAC"
BASE COUNT         1234 a         2701 c         2830 g         1029 t
ORIGIN      
        1 aagccccgcg gctgggtcat ggtgaacggc cagcgcgtcc cgtgggagac gtgccagacg
       61 ttctccggct cgtggggcta ccaccgcgac gaggccacct ggaagagccc ggcgcagctc
      121 atcgagctgc tcgtcgacac cgtcagcaag ggcggcaatc tgctgatgaa cgtcgggccg
      181 acggcgcgcg gcaccttcga ccagcgcgcg aaggaccgcc tggccgcctt cggccgctgg
      241 acgcaggcga acgggcggtc gatctacggg gcgacgcagg ctccgcccga gttcaaggct
      301 ccgccgaaca cgctgctgac gtacaacccg gcgacgaagc ggctgtacgt gcacgtgctg
      361 gcgtggccga tgggcgacat cgtcctcgag gggtacgccg gcaaggtggc ctacgcgcag
      421 ctgctgcacg atgcctcgga agtccggctg agcgagcggc gggccacgat ctggatggac
      481 gaagccggga agaccggtga cctcgtcatg cggctgccga tgctcaagcc cgacgtcgag
      541 atcccggttg tcgaactggt gctgaagtag ggacacccgc cggaagcggc cgcgcccggc
      601 tgaggccgcg ccgggcgcgc ccgatggcgt gctgcacgac cggcgccgca acgccgccac
      661 tccgcccacg gccgccgcac gccagctcgc ggggccgatc attgccggtt ccggacgcag
      721 gccgatgcgc gcgccggccg cccgaaagcg gcgcgcgcag cccgtcgctg cgcgccgccc
      781 gcggcggcgg gcccgcaggc cgccgtgata cgatgctcga tggccttgcg ccggcagggg
      841 gccgcagggg ccccggacgc gccgggaggc cggatgggca gccggttcgg gcgggacggc
      901 aggagacacg ggaggacggc atcatgagtt gtgcggcatg ctcggggcgg gcgatcgaca
      961 cggtcagggc gctcgagatt ctcgattcgc ggggcaatcc gaccgtgagc gtcacggtga
     1021 cgctggagaa cggcgtgacg gcgtcggccg cggtcccgtc tggcgcctcg accggggagc
     1081 acgaggccgt cgagctgcgc gacggcgaca agtcgcgcta tggcggcaag ggcgtcctga
     1141 aggccgtccg caacgtcaac gaggtgatcg ccccgaaggt cgagggcctg gacgcgacgc
     1201 gccagcgcga gatcgacctg ctcatgaagg acctcgacgg aacggccaac aagggcgtcc
     1261 tgggggcaaa cgcgatcctc ggcgtttcga tggcggtggc ccgggcggcc gcggcgtcga
     1321 gcgatctgcc gctctacgcg tacctcggag ggcccggcgc cacgcgcctc ccggtcccga
     1381 tgatgaacat cctgaacggc ggcgcgcacg ccaacaacag cgtggatctg caggagttca
     1441 tggtgatgcc gattggggcg ccgaccttcg ccgaggcgct ccgggccggc gcggagacct
     1501 ttcacgcgct caagggcatc ctgaagcagc gcggctacgc cacggccgtc ggcgacgaag
     1561 gcggcttcgc gcccgatctc aagagcaacg aagaggcgtg cgaggtgatc ctcgaggcga
     1621 tctcggcggc gggctacaag cccggccagg acgtgtcgat cgccctcgat ccggccgcct
     1681 cgtccttctt cgaggacggc gcgtacaacc tcgccaagtc gaggcagggc cggaagaccc
     1741 acgccgagat gaccgcaatc taccgcgact ggatcgccaa gtaccccatc gtctcgatcg
     1801 aggacggcct ggccgagaac gattgggatg gcttcaaggc gcacacggcg gcgctcggcg
     1861 accgcatcca gatcgtcggt gacgacctct tcgtcacgaa caccgcgttt gtcgcgcggg
     1921 gcatcgcgga gaaggcggcg aacgccgtgc tcatcaagct gaaccagatc ggcacggtct
     1981 ccgagacgat cgacacgatc gcgatgtgcc gcaaggccgg ctggggcttc gtgatctccc
     2041 accgctcggg cgagaccgag gacgccttcc tggcggactt cgccgtggcg atgggcggcg
     2101 gccagatcaa gaccggctcg gcatgccgca gcgagcgcat ggcgaagtac aaccgcctgc
     2161 tcgagatcga agccgagctg ggcgcggcag ccgtcttcac gaacccgttc gcgagatagg
     2221 gcgagagagg ggcggccgcc gccgggcagc gcgcgcggcg aggcagcctc cggccggacg
     2281 gccgggcgtc gggcccggcc gcccgcgcgg ttgcgggccg cgaggacggc ggtccgggcg
     2341 cgcccgtcgg gcgcccggag aagcccctca ggcgcgaagc ttacggctgc ccgggacggc
     2401 gctcgaacgc gtcggcgaac gcgcggcgct gctccaggct gccgagcatg accagccggg
     2461 catcggcccg cagcacggcg tctgcgtcga gcggcgtcga ggagccgtct ccgtgcttca
     2521 gcgccaccac gctcagcccc gtgcgggacc cgatctgcga ctcccgcagc ggcaggcccg
     2581 cgagcgacgc cgggacggga atcgagaaca gctctacgcc ctccccgagc agcaccagtt
     2641 cgtggccgcg caggatcgcc aggacggcgt cgacgccgag ggaggtgtag ctgaggacga
     2701 agtcggcgcc tgcacgatgg atcgcctcca ggttccgttc gtgggtgatc cggctgacga
     2761 tgcggatctc gggctgcagc cggcggcagt acaccgccag gaagatgttc atcgcatcgt
     2821 cgttcgtcgt cagcagcacc gaccgcgcgc tgccgattcc ggcgcggtcg aggagggcgc
     2881 ggtcggcggc gtctccctgg aaggcgcggt cgaccgtccc ggcgaggagc gcgagggcgg
     2941 cctcgtcacg ctcgatcacg tggacctgga ggcccttgcg cttgagggcc agggctgccg
     3001 cccggcccac ggtgccggcg ccaatgacga tcaccggccg gggctctgcg tcgctggccg
     3061 gcaggagccg gccgagtgct gcgatgtgcc cggccgtgcc cgcgaccact acgacgctgt
     3121 cgggccggat gagggtctgg ggatacgcgg gcagcaggcg cccgcgctcc cacagtccca
     3181 ccaggttgag gcccgtgcgc tcgcgcaggc gcgtgtcgcg gacggagttg ccggcgaacg
     3241 gcgttccgcg cgccggcagc tcggcgatca ggagatcgcc gaatgcgccg atcacgtggg
     3301 cctcggcggc gcccgcctcc acccggttcg cgaggtgctc acccagctgg tgcttgagcg
     3361 gaagcacgtg ggtggcgccg gccagccgca ggatgtcggc cgagtcctcg gcctcgacga
     3421 tcgcgacgat ggggacccgc ggcgcggcct cgcgcacggt gagcgtgatg ttcgtgttga
     3481 cggtgtccgt gcagttggcc agcaccaggc tcgcgcgttc gatcccgagc tgctcgtacg
     3541 tcgcccggct gtcgttccgg ccggccacca cggggacgcc gtcgttcacg agctggccgg
     3601 cgcgcacgac gtcgggctcg atgacgaagt gcggcacgcg ctcggaggcg agccgcgcgg
     3661 ccaggcccga ggcgacatcg tcgaactcgg caatcaggac gtggccggcc gtgccggccg
     3721 ggacgcggcg gggcgcgcgc aggcgaaccc gcgcttcgaa ccacggggcg tagaacaggc
     3781 ggatgaacag gaacgggagc aggacgagca ggagcaccac gcccgacagc agcacgacga
     3841 cgctgaaggc gcggccgacg tcggtcgtga acgtgatgtc gccgaacccg agggtcgtca
     3901 tcacgaccat cgtccagtag aagcccgtga cccacgaatg ccgctggcct tcaaggccgg
     3961 tcatgatgag gtggaacagg acgctgtgcg tggtgatgac cgccgccagc agcagcaggt
     4021 agcgcagcag cgcccggatg ttcccgcgca tctcgcgttc gctgatcagg taggcgatct
     4081 cgctgccgag gaacttcatc gtggcgctac tctaccactc cccccgcggc acggtcgcgg
     4141 tacaatccgg ctgccacgga ggagccatga gcatgagaag agccgtcctc gccctggcgt
     4201 tcaccggcgt cgccgccgcc gccgccgccc agccctacca gccgacctgg gagtctcttg
     4261 acgcgcgccc gacacccgcg tggttccagg acgcgaagtt cgggattttc atccactggg
     4321 gcgtctactc ggtgccggcg tacgcgcccg tcctgccagg caagctggcc tactcggagt
     4381 ggtactggaa cgccctcacg ggaggcaagg gggcgaagcc gagcccgatc caggcgggga
     4441 cgtgggagta ccaccgccag cgctacgggg ccgacttcag ctaccaggag ttcgcgccgc
     4501 agttccgggc cgagctgttc gacgccgacc actgggcgga ggtcttccgg cgatcgggcg
     4561 cgaagtacgt cgtgccgaca tcgaagcacc acgacgggtt cgccatctgg ccgagccgcg
     4621 aagcgtcggc gtcgtggggc cggccgtgga acgcggccga gatcgggccg aggcgcgacc
     4681 tgctcgggga gctgacggcg gccgtgcgcg cgaagggcct gcggatgggc ttctactact
     4741 cgctctacga gtggtacaac ccgatctggc tctacgacaa gccccgctac gttcgcgagc
     4801 acatgttccc ccagttcaag gatgtcgtga cgcgctacca gccgtcgatc atcttctcgg
     4861 acggcgaatg ggacctgccg tcgtccgagt ggaagacgcc tgagctcctc gcctggctct
     4921 tcaacgagtc gtcggtcaag gacgaagtgg tcgtcaacga tcggtggggc agggactgcc
     4981 gccacaagca cggcgggtac tggaccaccg agtacacgcc gggcatggcc gaggcgtccc
     5041 acccctggga agagagccga ggcatggggt tctcctacgg ctacaaccgc gccgagcgcc
     5101 tcgatcacta ccgcacgggc agggagctgg tgacgatcct cgtcgacacc gtcagccggg
     5161 gaggcaacct gctgctcgac atcgggcccg acgccgacgg cacgattccc gtgatcatgg
     5221 agcagcggct ggcggagatc ggcgcgtggc tcgacgtaaa cggcgaggcc atctacggca
     5281 cgcgcccgtt cacggccgcg aagcagtgga gcgccggcga ggtgccgacg atcgactaca
     5341 acaaggaata cgactcggac tacgacgtcg ccaggctcgt cgccccgtcc gcgccgggga
     5401 aggcctggat cgaggccttc ttcacgcaga agggcgacgc ggtgtacgcg atcctcacgc
     5461 gctggccggg acggcagttc gtgtggaaga acgccgcggg gctgaagccg aagtcggtca
     5521 cgctccttgg aggccgggat tcgatgcgcg tcaggaagtc cggcacggcg ctgatcgtcg
     5581 atctcccgga gctgcccgcg gagctgctcg gccagccgct gtgggtgctg aagctgtcac
     5641 gagagcgcgc gtcacgatga tccgatcggc gcggcgcgca gcgtgacgca caggatctcg
     5701 ccgcgccgcc acagccgcat gcgcggcccg aaggtgcccg tccctcggca gacgaggacg
     5761 ggcatgcccg cgacgtcgta gcgcccggcg aagagcggca tcacccggcg gacgatgagg
     5821 ccgaagggcc agatctggcc gccgtgcgtg tggcccgaga gcatcagccc cgcgccgagg
     5881 ctggcggcga catcggcgcc gacgggcatg tgcgagacga acacggtcgc ggccccgggc
     5941 ggccgccctt cgagcgcgcg cgcgatctgg ccggccggag gcacccggtt cgcccggatc
     6001 cccccgtcgc tcaccccggc gatgacgagt cccgggacga tctggaccca ctcgtcccgc
     6061 agcacccgga tcgcggcgtc gtcgaggggg ctcctcccgt cggctccgcg gccgtactcg
     6121 tcgtgattgc cggtgacggc gaacacgccg agcggcgccg tgatctgccg cagcgtcggc
     6181 agcatcgccc gcacccgctc gccgtcgccc tcgaagacgt ctccgccaat cacgacgatg
     6241 tccgggtcga gcgcgcccac ctgcgcgatg cgcgcccgca gccacgagtc gcccgtcatc
     6301 gtgcccaggt gaaggtccga gacgaagacg agaaccgtgc cgtcgcgcgc acccggcagc
     6361 gcggcgagcg tcacgtcgtg ccgccggacg acgggcgggc gcagcccttg gacgagcgcg
     6421 gcaacggaca gcgccgccgc ggcgaggagc gcccatcctc tgagggccgg gcggatgggc
     6481 gcggcccaca agccgaagcc cgttgccagc tccgcgacga gcaggcaggt cagcgccagc
     6541 cagatgatcc ccatccaggt cgcgccgacg acctcgagcg gcggggccag cgccctcagg
     6601 ggcgtcgccg ccagcaggcg cgaagcgggg aaggcgatcc cgagcgcggc gacggccgcg
     6661 gcgatggccc agcgcggcag gagttccgtc gcggcgggga tcgacagcag gtgccacagc
     6721 aggacggcat gcatcgatcc ccagatcgcc aggacgacga cgacgaacac cgcggcgctc
     6781 atggtccagg acgaccgcga cggcatcgtg agacccggcc gcgggtcacg agaccgcccg
     6841 cgtgccgcgg cctgcgcggg tcagcatgcg agcagttcct cgaggcgggc gatcgactgg
     6901 atcgcgccgc gcaccgagtg gtagttgatc ttccaggagt gcgacatgtg ggtccagatc
     6961 cgcgtgcggc cgtctcggct gaggagcggc caccactcgc cgacctcccg gttgaccatc
     7021 cggtccatga cgaaccggtg cacggcctcg tacgcctgcc agtacggctc catgccgaac
     7081 aggcggcagc cgtccagcat gccaatcatg gtttccgcct gctgccagaa ctccttctcg
     7141 gtgtcgtagg caggcccgcc gtcgtgaggc ccctccacgt agacgccgcc gtactcgaaa
     7201 tccacgccgg ccctgaccgc gtgatcgagc tgcttgcgga cgacgggctc gtacgggcgc
     7261 cggtctgcgc ccatcacgtc cagggcgtgc agcagcagcc aggcgcactc gacgttgtgg
     7321 ccgtaggagg tgttgtcgtc ggcgtgcggc ttggcgcccc cctcggcgaa gcggtcccag
     7381 ccccagacga tatcgaactt gatctggggg gcgacgcgcc agtccgccca gaactgcggg
     7441 attccggtcg cgtactgcgg gtgcaggatg cggcccacga ggaggtcgat gttctcctgc
     7501 agcgcgcgcc ggtgctcggc gcggcggctg cactcgaaca gcgtcgtgaa cgcctccatg
     7561 aggtgcatgt ggacgtcgag cgtcttccga tcgccgccgc cggcgcctgg tccgcacctc
     7621 gtccagtcgc gctcgaacat ctcgaaatag ccgccgagcg ccgggtcggc gcagtgccgc
     7681 tcgacgaggt cgaacgtccg ctgcgcgtac tcgatgccgc gcggatcgcc cgtcgccagc
     7741 gtgtactcgc tcaggctgta gatggcgaag ctgaggccgt acaggatctt cttg
//
LOCUS       DAOSIF010000083         7730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8700_length_7730_cov_4.998958, whole genome shotgun sequence.
ACCESSION   DAOSIF010000083 DAOSIF010000000
VERSION     DAOSIF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8700_length_7730_cov_4.998958"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLT35_04365"
     CDS             <1..754
                     /locus_tag="PLT35_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28492.1"
                     /translation="PEHRVTLGQMMEKLDEDAGLDASARVNTRENVRLTFDQKVEQVI
                     QEIVDSNFELYRRITDDRAFGEVVKNFLFDQYLRSHRNAEELIKRGESKTLEFKSTLR
                     WSLKEDRQDDKGVTHAALKAIAAFLNTEGGDLLIGVADDGSIVGIERDRLENDDKFMR
                     HLAQVVRNGLGDRAGTCIDPKMQVVQGRTVCVVSCQRSPEPVFLKWKGLEAAPDGDFF
                     VRSGPGTVKLPPESAREYIRTRFEARGAHRES"
     gene            1246..1761
                     /locus_tag="PLT35_04370"
     CDS             1246..1761
                     /locus_tag="PLT35_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28493.1"
                     /translation="MQEVARLLNSMREAGIIEDYALFGAAAQMRYTEAVATLDADVLV
                     VTATPDRLDVLEPIYAYCRERGYTPEGEAVRVGAWPVQFIPVFNSLTREAVSLADTAD
                     FEGVPFRVTGAAHLAVIALSVGRPKDFARVLELLESGAATQDDIGALAARHGLAETWT
                     RFRRKFLDDRD"
     gene            complement(2360..4114)
                     /locus_tag="PLT35_04375"
     CDS             complement(2360..4114)
                     /locus_tag="PLT35_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOG28494.1"
                     /translation="MPLRLRVLGAPLLQRVVGEGEQEVHVQAKRLAILAFLAVRRPPG
                     WCRRDTLLSLFWSERDGHDARNGLNQAVHQLRLGLGNRVISTRGHEEIRVEPGRLWCD
                     ALAFQDALAGGRCEDALDLYRGPFLDGLHVGDAPEFDGWLRAERERLRAQAAEAASLL
                     AHQERARGRTAVCVRWLQRLLALSPDDESALRQLMAVRRVLGDRPGALRDYQDFVRRL
                     SRELDLEHADGTRFLADAIRSEAGVPVPAVPRDQPADDAYWRGLVHWSRRTPDDLRQS
                     LYWFRQAMERNPSSCEAHAAFAMAGTALAAAFYDAEPADVLFPLVRSALTRALAIDGG
                     HASALAVLALVQGLYERRWREAEATSARACGLASHNTIVHHTRAVLAAYTGRLDDALV
                     SIEQAARLEPLALGYREIRGHYLCAARRYRQAAGDMEGLLRLEPRLYLARMALGHARA
                     ALGDTKAAEKAYRETLTAAGRHPYPLTSLAVLLARTGRARRAARLLDELLHLASSRYV
                     RPTYLAVVHAALGDHDSAFAALERAIAERDIHATSIVADPFADALREDPRFPPIVRRL
                     GLEPRRLPGVGFSGSVEG"
     gene            4445..4855
                     /locus_tag="PLT35_04380"
     CDS             4445..4855
                     /locus_tag="PLT35_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28495.1"
                     /translation="MRKLVLLVPAIALAAAPVAAQEAARPVNITGAWEMTVESPQGQM
                     VITANYRQEGETLTGSHVSEMGEAPLKGTVKGADVEYTLTIDMGGQQLAIVHKARIDG
                     DTLAGTADIEGFGSMAFTAKRKPSGPGGGGSQAR"
     gene            4888..6516
                     /locus_tag="PLT35_04385"
     CDS             4888..6516
                     /locus_tag="PLT35_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOG28496.1"
                     /translation="MRTLIPVAACAAALVAAAALPARAADRVTTANGIVESTAPPEGG
                     VRAFKGIPFAQPPVGDLRWREPQPVKDWGGVRNADRFGPACMQRLSPGADYWLRGDGM
                     SEDCLYLNVWTPAKSDREKLPVLVYIFGGGFQNGDGSEPRYDGANMARQGIVAVSLNY
                     RTNVFGFLAHPELTRESPHKASGNYGLLDQVAALRWVQKNIAAFGGDPKRVTIAGESA
                     GSISVSALMASPLSKDLIAGAICESGAAIASLPPRPLGEAEQAGVAFAAAAGAESLAA
                     LRAMTAQQIQDAAARAQGARFSTAMDGYFLARTLTDIGLAGEQARVPLLAGTNTEEMS
                     PRMVLAGGPPTPETLAAAITKLYGDKAGPVLKAYAATTTEEVFEAAMHLASARFISYG
                     TWKWTELHMQTSGKPVYRYLYARVRPAYLGMPGQPPPAPSAPPAPAPGAMPQPQPPRG
                     AAHSAEIQYAMGNLDLDRRYAWEPADYEVSKTMQAYFVNFIKTGDPNGPGLPPWPAYT
                     AATNYQRMLIDVTSQAGPEPHRDRYLALEAATGR"
     gene            complement(6562..7104)
                     /locus_tag="PLT35_04390"
     CDS             complement(6562..7104)
                     /locus_tag="PLT35_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28497.1"
                     /translation="MKHWLAAGMLLFAATAGAQDPAGAPRYPTSLPAKAGAPCGALRV
                     SALRGTYGFTATAWQDLSQINPQLPAGYAPVTIIGTFTIERDGVLTGWALINAGGLQM
                     AAEFVNSTVGAPRADCGIPITMSMKMAQFENAVMGPYSYVGVVTDDDGALELAFMMLG
                     TGPGSHVELDRARRISMRAQ"
     gene            7279..>7730
                     /locus_tag="PLT35_04395"
     CDS             7279..>7730
                     /locus_tag="PLT35_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOG28498.1"
                     /translation="MAAHCGRVAFGEFELDVEGETLYRSGRVLKLRPQPMRVLCLLVS
                     QAGKPVCRETIRRLLWGESTFVDYDVGMDYCVYRIRSVLGDKARSPRFIETLPRRGYR
                     FIAPIRRERAFNEPTLAVLPFVNLNRDPGKDYFADGVTDVLIAELACIP"
BASE COUNT         1233 a         2710 c         2731 g         1056 t
ORIGIN      
        1 ccccgagcac cgcgtgacgc tcggccagat gatggagaag ctcgacgagg acgccgggct
       61 cgacgcgtcg gcgcgcgtga acacgcgcga gaacgtgcgc ctcaccttcg accagaaggt
      121 cgaacaggtg atccaggaaa tcgtcgactc gaacttcgag ctctacaggc gcatcacgga
      181 cgatcgggcg ttcggcgagg tggtgaagaa cttcctcttc gaccagtacc tgcgcagcca
      241 ccgcaacgcc gaggagctga tcaagcgcgg cgagtcgaag acgctggagt tcaagtcgac
      301 gctgcgctgg agcctcaagg aggaccggca ggacgacaag ggcgtgacgc acgcggcgct
      361 caaggccatc gccgccttcc tgaacacgga gggcggagat ctgctgatcg gcgtggccga
      421 tgacggctcg atcgtcggca tcgagcgcga caggctcgag aacgacgaca agttcatgcg
      481 ccacctcgcc caggtggtgc gcaacggcct tggcgatcgc gccgggacgt gcatcgatcc
      541 gaagatgcag gtcgtccagg gaagaaccgt gtgcgtggtg agctgccagc gcagcccgga
      601 acctgtcttc ctgaagtgga aggggctcga ggccgcgccc gacggcgact tcttcgtccg
      661 aagcggcccg ggcacggtga agctgccgcc ggaaagcgcc cgcgagtaca tccggacgcg
      721 cttcgaggcc cgaggcgcgc acagggagtc gtgatgcagc tgcgattcag tacgatcgcc
      781 tcgaggccag acagccgcat cgggcgtaga acgtgcttcc cattcacgcc gtagcccgcc
      841 tgccccagcg cgacccgcat gcgtcgcgac ccgcatgcgt cgcggcccgc agaacggcgc
      901 cgccgcgtcc cggtcgtcca gcacccgcat cggggtgagc gccgtttcgc cgactgcggc
      961 cggcggacag gggaccgatc agggctccag gcccaggcgc cgcacgatgc gcggaaagcg
     1021 cggatcttcg cgcagcgcgt cggcgaacgg gtcggcaacg atcgacgtcg cgtggatgtc
     1081 gcgctcggca atggcccgct ccagcgcggc aaacgcgcgc cgaggtcgcc tccgcttcca
     1141 gccagcggcg ctcgtagagg ccctgcacca gggcgaggac ggcgagcgcc gcggcgtgcc
     1201 aacgccgcct tgcctcggtc gggcgatccc gctaccatca ggaccgtgca ggaagtcgcc
     1261 aggctgctga acagcatgcg cgaggcgggc atcatcgagg actacgccct cttcggagca
     1321 gcggctcaga tgcgctacac ggaggcggtc gcaacgctgg acgccgacgt cctggttgtc
     1381 acggcgacac ccgatcgcct cgatgtgttg gaaccgatct acgcctactg ccgtgagcgc
     1441 gggtacacgc ccgaggggga agcggttcgc gtcggtgcct ggccagtcca gttcatcccg
     1501 gtgttcaact cgctgacacg cgaggccgtg agccttgcgg atacggcgga cttcgaaggg
     1561 gtgccgttcc gggtcaccgg agcggcccat ctcgcggtca tcgcgctcag cgtcggccgc
     1621 ccaaaggact tcgcacgagt gctagagctg ctcgagtcgg gggcggccac gcaggacgac
     1681 atcggcgctc tagctgcgcg gcatggattg gctgaaacgt ggacgcgatt ccgccggaag
     1741 ttcctcgatg accgagactg aacggatgct gaggcggcaa gcggaatggc agaagacccg
     1801 ccaggcggca ccgtggccgg aaaagatccg gctggttgaa cggatgcgtc cgtcaatcga
     1861 ggcgatccgg ggggaacgaa agcgccactt ggacccgggg attccccaac ccgcgaacag
     1921 cggacgatct actcccgttt gatccgagtc ctcaatcacg tcatcggtgc tcgaagccct
     1981 gcctcccggc catccgcctc cgcggccgct cgatcgcacg gccgctacgg cgagaggtct
     2041 cgccgaagcc cgagccgtag cgggcgaagc agcgggcgcg gggcgaaggc ggacccccga
     2101 cgccgaattc gaacccttcc cgacagcatt cattagcgcg cgcgaagccg acacctggca
     2161 atttccgcca cggcgcccgc cccggggcgt tgcagaatcc gcgtagccgg gaagacaaag
     2221 agccgcataa ccctgcgttt accgcgcgta acacgccgcc gccgctcggc gcgagtattg
     2281 ctctcgctcg ggcatgcgcg gcatcgggtc ccagccgagc ccccacgccg ggtcccggta
     2341 cttcgcgatc gtctcggcgt cagccctcga cgcttccgct gaagcccact ccggggagcc
     2401 gccgcggctc caagcccagg cgccgcacga tgggcggaaa gcgcgggtct tcgcgcagcg
     2461 cgtcggcgaa cgggtcggca acgatcgacg tcgcgtggat gtcgcgctcg gcaatggccc
     2521 gctccagcgc ggcaaacgcg ctgtcgtggt cgccgagcgc ggcgtgcacg acggccaggt
     2581 aggtgggacg cacgtaacgg ctcgacgcca agtgcagcag ttcgtccagc agacgcgcgg
     2641 cgcgccgggc ccggcccgtg cgggcgagca acacggcgag cgaggtgaga gggtaggggt
     2701 ggcgccccgc ggcagtcagc gtctcgcggt acgccttctc ggccgccttc gtgtcgccta
     2761 gagcggcgcg ggcatggccg agtgccattc gcgccaggta cagccgcggc tcgagccgca
     2821 acaggccctc catgtcgccg gcagcctgac gataccggcg cgccgcgcac agatagtgcc
     2881 cccggatctc ccggtagccg agcgcgagag gctccagccg cgcagcctgc tcgatcgaga
     2941 caagcgcgtc gtccaggcgc ccggtgtagg ccgcgagcac cgcgcgcgtg tgatgaacga
     3001 tggtgttatg ggacgcgagc ccgcacgcgc gcgccgaggt cgcctccgct tcccgccagc
     3061 ggcgctcgta gaggccctgc accagggcga gaacggcgag cgccgacgcg tggccgccgt
     3121 cgatcgcgag cgccctcgtc aacgcgctcc tgaccagggg gaacaggacg tccgcgggct
     3181 ccgcgtcgta gaacgccgcc gcaagggccg tgcccgccat ggcaaacgcc gcatgcgctt
     3241 cgcagctgga ggggttccgc tccatggcct ggcggaacca gtagagcgac tggcgcagat
     3301 cgtccggcgt gcgcctcgac cagtgcacca ggccccgcca gtaggcgtcg tcggcaggct
     3361 gatcgcgggg gacggcgggc acggggacgc cggcctcgct cctgatcgcg tcggccagga
     3421 accgggtccc gtcggcgtgc tccagatcga gttcccgcga cagcctccgc acgaagtcct
     3481 ggtagtcgcg cagcgctccg ggtctgtcgc ccagcacgcg ccggacggcc atcagctgcc
     3541 gcagcgcgct ttcgtcgtcg ggcgagagcg cgagcaggcg ctgcagccag cggacacaga
     3601 cggccgttcg cccgcgcgcc cgctcttgat gggcgaggag cgaggccgcc tcggccgcct
     3661 gggcgcgcaa acgctcgcgc tcggcgcgca gccatccgtc gaactcgggg gcatcgccga
     3721 cgtgcagtcc gtcgaggaac ggtccccggt acaaatcgag ggcgtcctcg cagcggccgc
     3781 ccgcaagcgc atcctggaag gcgagggcgt cgcaccacag ccgccccggc tcgacgcgga
     3841 tctcctcgtg gccgcgcgtc gagatgacac gattccccag gccgagccgc agctggtgga
     3901 cggcctggtt gaggccgttg cgagcgtcgt gcccgtcgcg ctcgctccag aagagcgaga
     3961 gcagcgtgtc ccggcggcac cagccgggcg gacggcggac ggcgaggaag gccaggatcg
     4021 ccaggcgctt tgcctggacg tgcacttcct gctcgccttc cccgacgacc cgctggagaa
     4081 ggggcgcgcc gagcacccgc aagcgcagtg gcatgtgacg ccagcgaccg ggcatattat
     4141 gcggcggccc ggttcggcaa ggaagaggga gagtaggccg tccgcgtgct catggccggc
     4201 agaggagctg cctcatgcga agcggactgt tcggctgcgg cgtcgtcttc ggctgcatcc
     4261 tttctgccct ctccggctgc accagggccc ccgctgcgcc gagcgcgacg gcgaacccga
     4321 gcgcgacggc ggcggcgacg ccgtgggaag gcggctgtgc tattctccga cgcgtccagc
     4381 ccggcgcgcg cggcccggcg cggcggcagc ccgcgcgcgg ccaccggggg gcaaggagcg
     4441 acccatgagg aagctggttc tgctcgttcc ggccatcgcg ctggccgcgg cgcctgtcgc
     4501 cgcgcaggaa gccgcaaggc cggtgaacat cacgggcgcg tgggagatga ccgtggaatc
     4561 gccccagggg cagatggtca tcaccgccaa ctaccggcag gagggcgaga cgctgacggg
     4621 cagccacgtc agcgagatgg gcgaggcgcc cctgaaaggc accgtgaaag gcgcggacgt
     4681 cgagtacacg ctcacaatcg acatgggcgg ccagcaactg gccatcgtgc acaaggccag
     4741 gatcgacggc gacaccctgg ccggcacggc cgacatcgag ggcttcggct ccatggcctt
     4801 cacggcgaag cggaagccgt cggggccggg cggcggcggg agccaggcgc gatgactcgc
     4861 gggaacccgg ggcctctggg agcttccatg cgcacgctga ttcccgttgc tgcctgcgcc
     4921 gccgccctgg tcgccgcggc ggcgctgccg gcccgcgccg ccgatcgcgt gacgaccgcc
     4981 aacggcatcg tcgaatccac cgcgccgccg gagggcggcg tccgcgcctt caagggcatt
     5041 ccgttcgcgc agccgcccgt cggcgacttg cgctggcggg agccgcagcc ggtcaaggac
     5101 tggggcggcg tgcgcaacgc ggacaggttc ggccccgcct gcatgcagcg gctgtcgccg
     5161 ggagcggact actggctgcg cggcgacggg atgagcgagg actgcctgta cctgaacgtg
     5221 tggacgccgg cgaagtcgga ccgcgagaag ctgccggtcc tggtctacat cttcggcggc
     5281 gggttccaga acggcgacgg ctccgagccg cggtacgacg gcgcgaacat ggcccggcag
     5341 ggcatcgtcg cggtgtcgct gaactaccgc acgaacgtct tcggcttcct cgcgcacccc
     5401 gagctgacgc gcgagtcgcc gcacaaggcc tcgggcaact acggcctgct cgaccaggtg
     5461 gctgcgctgc gatgggtgca gaagaacatc gccgcgttcg gcggcgatcc gaagcgggtg
     5521 acgatcgcgg gcgagtcggc gggatcgatc tcggtcagcg ccctcatggc gtcgccgctc
     5581 tcgaaagatc tgatcgccgg cgccatctgc gagagcggcg cggccatcgc cagcctgccg
     5641 ccgcggccgc tcggcgaggc ggagcaggcc ggcgtcgcgt ttgccgccgc cgccggcgcg
     5701 gagtcgctcg ccgcccttcg cgccatgact gcgcagcaga tccaggatgc cgccgccagg
     5761 gcgcagggcg cccggttcag cacggcgatg gacggatact tcctcgccag gacgctgacc
     5821 gacatcgggc tcgctggtga gcaggccagg gtcccgctgc tcgcggggac gaacacggag
     5881 gagatgagcc cgcgcatggt gctcgccggc ggcccgccca cgcccgaaac gctcgcggcg
     5941 gccatcacga agctctacgg cgacaaggcc gggccggtgc tgaaggcgta tgcggcgacg
     6001 acgacggagg aggtgttcga ggcggcgatg caccttgcga gcgcgcggtt catctcctac
     6061 ggcacgtgga agtggaccga gctgcacatg cagacgagcg gcaagcccgt gtaccgctac
     6121 ctctatgcca gggtgcggcc cgcgtatctc ggcatgccgg gccagccgcc gccggcgcct
     6181 tccgcgcctc ccgcgccggc tcccggcgcg atgccgcagc cgcagccgcc ccggggcgca
     6241 gcgcattcgg ccgagatcca gtacgccatg ggcaacctcg atctcgatcg gcggtacgcg
     6301 tgggagcccg ccgactacga agtgtcgaag accatgcagg cgtacttcgt gaacttcatc
     6361 aagaccggcg atcccaacgg cccgggcctg ccgccgtggc cggcctacac ggccgccacg
     6421 aactaccagc gcatgctgat cgacgtgacg tcacaggcgg gccctgagcc gcaccgcgac
     6481 cggtatctcg cgctggaggc ggctaccggg cgatagggcc cgccgccttc cgccggccct
     6541 ggcccccggc gccgagcgcg cctactgggc cctcatcgag atccgtctcg cgcggtcgag
     6601 ctccacgtgc gaccccggcc cggtgccgag catcatgaac gctagctcga gggccccgtc
     6661 gtcgtccgtg acgaccccca cgtacgagta gggtcccatg accgcgtttt cgaactgcgc
     6721 catcttcatc gacatcgtga tgggaatgcc gcagtcggcc cgaggcgcgc ccaccgtcga
     6781 gttgacgaac tcggcggcca tctgcagccc gcccgcgttg atgagcgccc acccggtcag
     6841 gacgccatcg cgctcgatgg tgaaggtgcc gatgatcgtc accggagcgt agccggccgg
     6901 aagctgggga ttgatctgcg agagatcctg ccacgcggtg gcggtgaagc cgtacgtgcc
     6961 ccgcagcgcc gacacgcgca acgcgccgca cggcgcgccc gctttggctg gcagcgaggt
     7021 cggataccgg ggagcgcccg ccgggtcctg cgccccggcc gtcgccgcga acaacagcat
     7081 gccagccgct agccagtgct tcatgatgcg tgtctccttt caaatggccg tggccggagg
     7141 ccaatccggc cccctgccga cacgcgtacc gtagactccg ttcgccgggt gttccatcga
     7201 cagcggctgg gaacttgctg gatttgtcct gtgcgcccaa cgtggtggta ggattgggct
     7261 tgcagagaac cagaccacgt ggcggcgcac tgcggtcgtg tcgctttcgg cgagttcgag
     7321 ctggacgttg agggcgaaac cctgtacagg tccggcaggg tgctgaagct gcgcccgcag
     7381 ccgatgcgcg tgctctgcct gctcgtcagc caggcgggca aaccggtctg ccgcgagacc
     7441 attcgccggc tcctctgggg cgaatcgacg ttcgtggact acgacgtcgg gatggattac
     7501 tgcgtctacc gcatccgctc cgttctcggc gacaaggcgc ggtcgccccg gttcatcgaa
     7561 acgcttcccc ggcgcgggta ccgcttcatc gcgcccatca ggcgcgaacg ggcgttcaac
     7621 gagcccaccc tcgccgtgct gccgtttgtc aatctcaacc gcgaccccgg caaggactac
     7681 ttcgccgacg gcgtgaccga cgtgctcatc gccgagctcg cgtgcattcc
//
LOCUS       DAOSIF010000084         7723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8706_length_7723_cov_4.232525, whole genome shotgun sequence.
ACCESSION   DAOSIF010000084 DAOSIF010000000
VERSION     DAOSIF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8706_length_7723_cov_4.232525"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1731
                     /locus_tag="PLT35_04400"
     CDS             181..1731
                     /locus_tag="PLT35_04400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG28499.1"
                     /translation="MPADPPRQIAPLPAAWAPACVVLPLATLVFQLLTHRGYGFFRDE
                     LYYLASAEHLGLGYVEHPPMIALVAWLVRSALGDSLLAARLLPAVAHGALVALAAMLA
                     REMGGGRRAQLLAALATAVAPAYVGLCGILSMNAFDLVFWTALWLVAARALRTGDTRW
                     WLWFGVIAGAGLQNKISVLFLGFGTVAGLAVTRRWRVLRSRHLWVGGAVAALVFLPHL
                     VWQWQNGWPTLEFIANATREKNVALSPGAFLGEQVGTMGALAAPLWIGGLAGLLAARR
                     LGTFRPLGWAYPVVLAVMLASNSKPYYLTPAYTVLFAAGAVLVEGIRWSRVRSIAAPA
                     LAVMIALGGAMSLPLVKPVLSTDAFLRYSAAIGQTPRPEERHEMGRLPQMYADMHGWP
                     ELAANLATVYHALPARDRERACIFVRNYGEAGAVDVLGAPLGLPRAISGHNSYFLWGP
                     RGCTGDLVIALGAGRGDYEEAFASVEQAGVHTCTDCMPYENNLSIWVLRGARVPFAAY
                     WPRVKRYI"
     gene            complement(1769..3667)
                     /locus_tag="PLT35_04405"
     CDS             complement(1769..3667)
                     /locus_tag="PLT35_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOG28500.1"
                     /translation="MPQDYRRPLDLAVDAALKAGALLRDEFHRAGGPRGAGAHAEADT
                     EAERLIRHALVPAFPGWGYLGEETGAQPRAPGEAHHWLVDPNDGTRDYLKGRRGSAVS
                     IALLRDGVPVLGVVYAFAAPDDRGDLFAWADGCGPMERNGRAVERPAWPTALGRYDVV
                     LLSAAMNRKPRPNLELIQPARARAVPSIAYRLALVAAGDGDAAVSLNGPGAWDFAAGH
                     ALLGSAGGDFVDQDGRRIVYSADGSSRARFCFGGAPALVRDLASRAWQAVLDAPEPAA
                     EPYDLVTPEPGGATADSGLLSRAQGCLLGQVAGDALGAYVEFKTPLELARRHPGGLRK
                     MADGGTWNTLAGQPTDDSELALMLARSIVSTGRFDAEAAARAYHHWYHSRPFDCGGTT
                     AKALAAIGPGDVERGQTAAAAAQAASRESQANGSLMRCSPLAVWAHALDAERLAAAAR
                     EDSALTHPHPVCCDAVTLFAVAVAHAVRTGERPAQVYAFAMKRAEDLRCRRDVRRVVG
                     LAATEPPASFMDRQGWVLVSLQNAFHQLLHAESVEEGVVRTVMAGGDTDTNAAIAGAL
                     LGAVHGRDAIPHAWRQMVLTCRPLAGLPSVRHPRPRAFWPVDLLELAERLLSLGARAD
                     GTASERGQ"
     gene            3918..4856
                     /gene="thyX"
                     /locus_tag="PLT35_04410"
     CDS             3918..4856
                     /gene="thyX"
                     /locus_tag="PLT35_04410"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOG28501.1"
                     /translation="MSDDISRPETIARPVVPALEAVLGRPFRVLDDGFVRVVDYMGDD
                     SAVVQAARTSYGAGTKRRHEDRGLIRYLMRHAHTTPFEMCELKLHVRVPMDVWRQWIR
                     HRTASVNEYSTRYSVAIDAAQRTAPDAWRLQSPANRQGSAGLLHAGQGAALSEEESRL
                     QHAARAAYQARLAAGVAREQARKDLPLSTYTEAYWKIDLQNLLHFLDLRMDARAQQEI
                     REYATVIGEQIVAKWVPLTWEAFLDYRRGGLVLSRIENEFLAALTSGDRARAIAIADE
                     GGLLTHAEDGRLKANLEGRELEDKLARLGLRAPWTE"
     gene            complement(4939..5784)
                     /locus_tag="PLT35_04415"
     CDS             complement(4939..5784)
                     /locus_tag="PLT35_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOG28502.1"
                     /translation="MIGAITGDVVGSVYERTRPGAKAVPLLSSRSRFTDDSVLTIAVA
                     SAILHGEDYGSSIRKWARRYPYAGYGGRFKKWMSNDEAGPYGSYGNGSAMRVSAVGWA
                     FESLDCVLAEAERTASPTHNHPEGVKGAQAVASAVFLARTGSTAAQIDRFLTDRFGYD
                     CRASLEKARREPHFDVTCQGTVPTAAAVAFEASGVEGAIRSAVALGGDADTIACIAGA
                     IAEALHGGVPFDIQAGVFGRLDDTLRTVAVEFALKYRLSLAPSLRGPGQPARGSRGSG
                     MRWKS"
     gene            complement(6193..6402)
                     /locus_tag="PLT35_04420"
     CDS             complement(6193..6402)
                     /locus_tag="PLT35_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6370..6372),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HOG28503.1"
                     /translation="MHVLIFGKDTUPYTSEAREVYAKRGYEVEYVNAKASAANLQRML
                     GYTKGGRDVPVIVEGDKVTIGYGGT"
     gene            complement(6444..6875)
                     /locus_tag="PLT35_04425"
     CDS             complement(6444..6875)
                     /locus_tag="PLT35_04425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG28504.1"
                     /translation="MTESRFPVHCPIEVRFRDCDMMGHVNNAVYLTYLEVARFAYWRA
                     AGLDRVADEVSYILARVEIDYRAQAEVGEVLDVAAGVTGIGRSSFSMAFEIRNPAGRL
                     VAAAKSVQVAYDYGARAPVPVPDAVRARIAEFEGKQYSSSV"
     gene            complement(6886..>7723)
                     /locus_tag="PLT35_04430"
     CDS             complement(6886..>7723)
                     /locus_tag="PLT35_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG28505.1"
                     /translation="FGAAFGLGFIFGPAIGGFLSRWGYSAPAFFASGLALANFTAALF
                     LLPETLKPEHRAIRRAGRLDALRAAMAKPHLPLLMLIGFLAVAAFASFETTFALYSEH
                     VHGFTASTIGYVFALIGVVMVIVQGGLVGRAVKLVGEHHLVPVSLAVVAAGLFLIATS
                     RSAAVVVAANAVMAAGMGFNNPALASLISRCSSAEDQGGVMGLVQSLNSLARIAGPLW
                     GGYAFDRLGVVMPYFSGSAVMAVAAALAVRALWKARLAAAPETVAIVSMAAGSSARSP
                     ES"
BASE COUNT         1088 a         2815 c         2740 g         1080 t
ORIGIN      
        1 caaccgagac catttcgccg cgcccaatcg acagatcgac gcggtcgagg gcgatgaccg
       61 tgcgcctgcc gttgaaggcc ttcgtgacgc ccgagagggt gatcatgcct ggccctatcc
      121 tgccacgcgc gggcgaacct cgtcgaggcg cccggccgcc cccgggtgta acatggccga
      181 atgcctgccg acccgcctcg gcagattgcg cccctgccgg ccgcgtgggc gccggcgtgc
      241 gttgtcctgc cgctcgccac cctggtcttt cagctgctga cgcaccgcgg gtacgggttc
      301 ttccgcgacg agctgtacta cctcgcgtcg gccgagcatc tcgggctcgg gtatgtcgag
      361 cacccgccga tgatcgccct tgtggcctgg ctggtgagat ccgcgctcgg cgattcgctg
      421 ttggcggcgc ggctgctgcc ggccgttgcg cacggggcgc tcgtggcgct cgccgcgatg
      481 ctcgcgcgcg agatgggcgg cgggcgacgc gcgcagttac tcgccgcgtt ggcgacggcc
      541 gtggcgcccg cctacgtcgg cctgtgcggc atcctgtcga tgaacgcgtt cgatctcgtg
      601 ttctggaccg cgctgtggct cgtggcggca cgcgccttgc gcaccggcga cacgcgctgg
      661 tggctgtggt tcggcgtgat cgcgggcgcc ggcctgcaga acaagatcag cgtcctcttc
      721 ctgggcttcg ggaccgtcgc cggtctggcc gtcacgcgcc ggtggcgggt cctgcgctcg
      781 cggcatctgt gggttggcgg cgccgtcgcc gccctcgtct tcctgccgca cctcgtatgg
      841 cagtggcaga acggctggcc gacgctcgag ttcatcgcga acgccacgcg cgagaagaac
      901 gtggcgctct cgcctggcgc cttcctcgga gagcaggtcg gcaccatggg cgcgctggcc
      961 gcgccgttgt ggatcggcgg gctggccggc ctgctggccg cgcggcggct tggcacattc
     1021 cggccgctcg gctgggccta tcctgtcgtg ctcgccgtga tgctcgcgtc gaactcgaag
     1081 ccgtactacc tgacgcccgc ctatacagtg ctcttcgcgg ccggcgcggt cctcgtcgaa
     1141 ggcatccgct ggtcacgggt gcgctcgatc gcggccccgg cccttgccgt gatgattgca
     1201 ctcggcggcg ccatgtcgct gcctctcgtc aagccggtgc tgtccacgga tgcgttcctg
     1261 cgctactcgg cggccattgg acagacgccg cgcccggagg agcgccacga gatggggcgg
     1321 ctgccgcaga tgtacgccga catgcacggc tggccggagc tcgccgcgaa cctggcgacg
     1381 gtgtaccacg ccttgccggc gagagatcgc gagcgcgcct gcatcttcgt gcggaactac
     1441 ggcgaagccg gggccgtgga cgtcctgggc gcgccgctcg ggctgccgag ggccatctcg
     1501 ggccacaaca gctacttcct ctggggcccc aggggctgca cgggcgacct cgtcatcgcg
     1561 ctgggcgccg gccgaggcga ctacgaggag gcgttcgcgt cggtcgagca ggccggcgtg
     1621 cacacgtgca ccgactgcat gccctacgag aacaacctct ccatctgggt cctgcggggc
     1681 gcgcgcgtgc cattcgccgc gtactggccg cgcgtgaagc gctacatctg aaaggagcgg
     1741 ccgcggccgc cgcctgagcc cggcgcagtc actgtccccg ctcggatgcc gttccgtcgg
     1801 ctctcgcgcc aaggctcagc aggcgctcgg ccagctcgag aaggtcgacg ggccagaacg
     1861 cgcgcggccg cgggtggcgg acggagggca ggcccgcaag cggccggcac gtcaggacca
     1921 tctggcgcca ggcgtgcggg atcgcgtccc ggccgtggac ggctccgagg agcgcgccgg
     1981 cgatggcggc gttcgtgtcg gtgtcgccgc ccgccatgac cgtccgcacg acgccttcct
     2041 cgacgctctc cgcgtgcagc agctggtgga aggcgttctg gagcgacacc agcacccagc
     2101 cctggcgatc catgaagctg gcgggcggct cggtggcggc caggccgacc acgcggcgca
     2161 cgtcgcgccg gcacctgagg tcctcggccc gcttcatggc gaaggcgtag acctgcgccg
     2221 gccgctcccc cgtccgcacg gcgtgcgcca ctgcgacggc gaacagcgtc accgcatcgc
     2281 agcacaccgg gtgcgggtgc gtgagcgccg agtcctcgcg cgcggcggcg gccagacgct
     2341 ccgcgtcgag cgcgtgggcc cagacggcca gcgggctgca gcgcatcagc gagccgttcg
     2401 cctggctctc gcgcgaagcg gcctgcgcgg cggccgccgc cgtctggccg cgctccacgt
     2461 ctcccgggcc gatggccgcc agcgcctttg ccgtcgtgcc tccgcaatcg aaggggcgcg
     2521 agtggtacca gtggtggtag gccctggccg cagcctccgc gtcgaaccgc cccgtgctca
     2581 cgatcgatct cgccagcatc agggcgagct cgctgtcgtc ggtcggctgg ccggccagcg
     2641 tgttccacgt gccgccgtcg gccatcttcc ggaggccgcc cgggtgccgg cgcgccagtt
     2701 ccagcggggt cttgaactcg acatacgcgc cgagggcgtc gccggccacc tgcccgagca
     2761 ggcagccttg tgcgcgggac aggaggccgg agtccgcggt ggcgccgccc ggttcaggcg
     2821 tcacgagatc gtagggctcg gctgccggct ccggagcgtc gagcaccgcc tgccacgcgc
     2881 gcgaagcgag gtcgcgcacg agagccggcg cgccgccgaa gcagaaccgc gcgcggctcg
     2941 acccgtcggc cgagtagacg attcggcggc cgtcctggtc gacgaagtcg ccgcccgcgc
     3001 tgccgaggag cgcgtggccg gcggcgaagt cccaggcgcc ggggccgttc agcgacaccg
     3061 ccgcgtcgcc gtcgccggcg gcgacgagcg ccaggcggta cgcgatgctc ggcacggcgc
     3121 gcgctcgcgc cggctggatg agctccaggt tcggccgggg cttgcggttc atcgcggccg
     3181 acagcagcac gacgtcgtag cggccgagcg ccgtcggcca cgccggccgc tcgacggccc
     3241 ggccgttgcg ctccatcggt ccgcagccgt ccgcccaggc gaacagatcg ccccggtcgt
     3301 cgggcgcggc gaaggcgtag accacgccga ggaccggcac gccgtcgcga agcagggcga
     3361 tcgaaacggc gctgccgcgg cgccctttca ggtagtcgcg ggtgccgtcg ttgggatcga
     3421 cgagccagtg gtgcgcttcg ccaggggcgc gcggctgcgc gccggtctcc tcgccgaggt
     3481 agccccagcc cgggaacgcc gggaccaggg cgtgccggat gagccgctcg gcctccgtgt
     3541 ccgcctccgc gtgcgcgccc gcgccgcgcg ggccgcccgc gcggtggaac tcgtcgcgca
     3601 ggagcgcgcc ggccttgagc gccgcgtcca cggcgaggtc gagcgggcgc cggtaatcct
     3661 ggggcacggt gcctccccgt gcggaaaggg cggtgaacgc cggcgcgatg gcggggcgag
     3721 ccggatttta ctccccgcca ccggccttgt cgagcgggcc tggcggcggc tgccggtgtt
     3781 cgacggaacc gcttcgcgct gatcttggcg gcgatggacc gccgcggcgc cggcatccag
     3841 aaggcctggg cccggcaagc cagacccatc ggccggcgcc agccgcggta gaatgccgga
     3901 cagggagccc ttggcaaatg agcgacgaca tctctcgccc cgagaccatc gcccgccccg
     3961 tcgtgcccgc cctcgaagcg gtgcttggcc gccctttccg cgtcctcgac gacgggttcg
     4021 tgcgcgtcgt ggattacatg ggagacgact cggctgtcgt gcaggcggcg cgcacgtcgt
     4081 acggcgcggg cacgaagcgc cggcacgagg accgcggcct catccgctac ctcatgcgcc
     4141 atgcgcacac gacgcccttc gagatgtgcg agctgaagct gcacgtgcgc gtgccgatgg
     4201 acgtctggag gcagtggatc cgccaccgca cggcgagcgt gaacgagtac tcgacgcggt
     4261 actccgtggc catcgacgcg gcccagcgga cggcgccgga cgcctggcgg ctgcagtcac
     4321 cggcgaaccg ccagggcagc gccgggctgc tgcatgccgg gcagggcgcg gcgctgtccg
     4381 aggaggaatc gcggctccag cacgccgcgc gcgccgccta ccaggcccgc ctggctgcgg
     4441 gcgtggcccg cgagcaggcg cgcaaggacc tgccgctctc cacctacacc gaggcctact
     4501 ggaagatcga cctgcagaac ctgctgcact tcctcgacct gcggatggac gcgcgcgcgc
     4561 agcaggagat ccgcgagtac gccaccgtca tcggcgagca gatcgtggcc aagtgggtgc
     4621 cgctcacctg ggaggcgttc ctcgactacc ggcgcggcgg cctcgtgctg tcgcggatcg
     4681 agaacgagtt cctcgccgcg cttacgtccg gcgatcgcgc gcgcgccatc gccatcgcag
     4741 acgagggcgg cctgctgacg cacgcggaag acgggcgcct caaggcgaac ctcgagggtc
     4801 gggagctcga ggacaagctg gcgcgcctgg gcctgcgggc gccgtggacc gagtagcggc
     4861 gggccgcgcc ggctgacgcc gcatcgcgct gtctcttcgc ctccggcggg ttcccgtacg
     4921 acctccttcg ctcgcgggtc aggacttcca ccgcatcccg cttccgcgtg aaccgcgcgc
     4981 gggctgcccc ggccctctca ggctcggtgc gagagacaat cggtacttca gcgcgaactc
     5041 caccgccacc gttctcagcg tgtcgtcgag ccgcccgaag acccccgcct ggatgtcgaa
     5101 cgggacgccc ccgtgcagcg cctccgcaat ggcgccggcg atgcacgcga tcgtgtccgc
     5161 gtcgccgccc agggccaccg ccgagcggat ggcgccttcc acccctgacg cctcgaatgc
     5221 gaccgcggcc gccgtcggca ccgtgccctg gcacgtcacg tcgaagtgcg gctcacgcct
     5281 cgccttctcc aggctcgccc ggcagtcata gccaaagcga tccgtcagga agcgatcgat
     5341 ctgggccgcg gtgctgcctg tccgggcaag aaagacggcg ctcgccaccg cctgcgcccc
     5401 cttcacgccc tcggggtgat tgtgggtcgg gctggctgtc ctctccgcct ctgccagcac
     5461 gcagtcgaga ctctcgaagg cccatcccac cgccgatacg cgcatcgccg agccgttgcc
     5521 atagctcccg taggggccgg cttcgtcgtt ggacatccac ttcttgaacc ggcctccgta
     5581 cccggcgtag gggtatcgac gcgcccactt ccgaatggag gaaccgtagt cctcaccatg
     5641 gaggatcgcg ctggcgacgg cgattgtgag cacggagtcg tcggtgaagc gagatcggga
     5701 tgagaggagc ggaacggcct tcgcgcccgg cctggttctc tcgtagaccg atcccacgac
     5761 gtcgcccgtt atagcgccaa tcatcggctg ccctccgcgc gccggccagc cgcgcgggcg
     5821 ccgcaggcgg ccccgtcgcg acaccgcgga ccaaccggcg atcgagaacc acatgttctc
     5881 gacactgaac actccgacgc cgcgcagaat cccccgggaa tctgcatctg tatccagcaa
     5941 agccgtacta cgagaacgat gggccatcgg cagcctgaag ccgcacggca acaggcaggc
     6001 agcgatggcg acatgtcagt cggtacgccc ctggctggac acgatgcagc cgcagttcga
     6061 gcggcgcccg gccgacgcct ggggccagga tggagtccgg gccgcgcacg gcggcccctc
     6121 agaccggctt cacagcccgt tctcgcggcg cagccgcgtc acgcaggccg ggaagccgtc
     6181 cccttacacc cctcaagtgc cgccgtaccc gatggtgacc ttgtcgccct cgacgatgac
     6241 cgggacgtcg cggccgccct tggtgtagcc gagcatgcgc tgcaggttcg ccgcgctcgc
     6301 cttggcgttg acgtactcga cctcgtagcc gcgcttcgcg tagacctcac gggcctctga
     6361 ggtatagggt caggtgtcct tgccgaagat gaggacgtgc atcgaagacc tccaggcgct
     6421 aggggctcgg cgccaggcgc tggtcagacg gacgacgagt actgcttccc ttcgaactcg
     6481 gctatgcgcg cgcggacggc gtcgggcacc ggcacgggcg cccgcgcgcc gtaatcatac
     6541 gcgacctgga cgctcttcgc cgcggccacc aggcggccgg ccggattgcg gatttcgaag
     6601 gccatcgaga agctcgatcg cccgatcccg gtgacgccgg cggcgacgtc cagcacctcg
     6661 ccgacctccg cctgcgcgcg gtagtcgatc tcgacgcgcg ccaggatgta gctcacttcg
     6721 tccgcgacgc ggtcgaggcc cgcggctcgc cagtaggcaa agcgcgcgac ctccaggtac
     6781 gtgaggtaga cggcgttgtt gacgtggccc atcatgtcgc agtcgcggaa ccggacctcg
     6841 atggggcagt gaacgggaaa gcgcgattcg gtcatgacgc gggactcagg actcgggact
     6901 ccgggcgctc gatccagcgg ccatcgacac gatagcgacg gtctcgggcg ccgccgccag
     6961 ccgcgccttc cacaacgcgc ggaccgcgag cgccgcagcc acggccatca cggccgaccc
     7021 gctgaagtag ggcatcacga cgccgaggcg gtcgaacgcg tagccgcccc agagcggccc
     7081 cgcaatgcgc gcgaggctgt tcagcgactg caccagcccc atcacgcctc cctggtcctc
     7141 ggccgacgag caccgggaga tcagcgacgc cagcgcgggg ttgttgaacc ccatgccggc
     7201 cgccatcacg gcgttggccg cgacaaccac ggccgcggat cgggacgtcg cgatcaagaa
     7261 cagcccggcc gccacgacgg ccagcgacac gggcacgagg tggtgctcgc cgacgagctt
     7321 cacggccctg ccgacaaggc ctccctggac gatgaccatg acgacgccga tcagggcgaa
     7381 cacgtagccg atcgtcgagg cggtgaagcc gtggacgtgc tccgagtaca gcgcgaacgt
     7441 cgtctcgaag gaggcgaagg cggccacagc caggaacccg atgagcatca gcagcggcag
     7501 gtgcggcttc gccatcgccg cgcgcagcgc gtcgagccgg ccggcccgcc tgatggccct
     7561 gtgctcgggc ttcagggtct cgggcagcag gaagagcgcg gccgtgaagt tcgccagcgc
     7621 gaggccggag gcgaagaacg cgggcgccga gtacccccaa cggctcagga acccgccgat
     7681 ggccggcccg aagatgaagc ccagcccgaa ggcggccccg aac
//
LOCUS       DAOSIF010000085         7715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8719_length_7715_cov_3.479634, whole genome shotgun sequence.
ACCESSION   DAOSIF010000085 DAOSIF010000000
VERSION     DAOSIF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8719_length_7715_cov_3.479634"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="cutA"
                     /locus_tag="PLT35_04435"
     CDS             <1..371
                     /gene="cutA"
                     /locus_tag="PLT35_04435"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOG28506.1"
                     /translation="YIGKAMESAPITRDDLDASAVVVVLTTLPAGADALAFARAVVDE
                     RLAACVGVLSEMQSVYRWQGAVEHAPERQLVIKTTRGQVARLTDWLAAHHPYEVPELI
                     VMPADGGDAYLAWVRQSTAG"
     gene            394..2553
                     /locus_tag="PLT35_04440"
     CDS             394..2553
                     /locus_tag="PLT35_04440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG28507.1"
                     /translation="MASTLTFSNPAGRLGAWAAAARRFLLTTVEGRLIVGGAAVKVAV
                     NGIAFAGLGASRILEGISTAGGLAILAGVLGLLYRAWRGVRHQLLWRVRRKLIISYVF
                     IGVVPVILIIAFFVLAWLLLFANISSYLIRSALVDLGEGAMSAARLAAVEIEDRADPA
                     RVSAILERRAAAEAVRHAGISLAVVPIDADRCPAPADGAPARAVAAASAGSWAHTLAP
                     GLVPAWVPCDGFAGVLAYGARPGPEAPAAEVFVRAVAFPERSRGFVVIADVPVSRPVK
                     AAIEAETGIVIGEASLVGSTSLLVRGTAGTPAGDLPQASTAAERVWRFPWVALLDHVD
                     WTTGRTGPLPINIGLSVRAIYQRLSASQASLGGTRSFGDLLLALLLFVSGLFFIIEVV
                     ALVMGLALARSITGAIHELFAGTERVRQGDFGHRIPVLARDQLGELASSFNQMTGSIE
                     DLLRQAAEKKRLEEEMRLAREIQMSLLPRGPLVVPGVAISALCVPAREVGGDYYDVLS
                     LPDGRFGVLIADVSGKGMSAALYMAELKGLILSLSQVHLSPRELLIGANRLISMHLDS
                     RSFITMTYAVIDPAAGVMTYARAGHTPLLYLPGRGADGRARTLAPDGMIVGLRLDDGE
                     RFKSLLVESTLRLAAGDLLVFFTDGVSEAMNDRADCFGEAQLAELVEQHAHLPIGELR
                     ERILREITAFVGEAPQHDDMTLILLKVRGDEQAAGAS"
     gene            2550..3494
                     /gene="rnc"
                     /locus_tag="PLT35_04445"
     CDS             2550..3494
                     /gene="rnc"
                     /locus_tag="PLT35_04445"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOG28508.1"
                     /translation="MTDPVVVLRTHSDIEASIVIGLLDAHGIPASLASDVPHAVFPLT
                     INGLGEVRVSVPGNRAEEASRILADYRRAGGAERAVKIGEEFEALEAAIGHRFKDRGT
                     LEHALTHRSRANEDVTGGVFDNESLEFLGDAVLGFVIAETLFVRFPDRDEGKKSKMKA
                     ALVSTQTLAGQAERLGLGAHLLLGRGEEKTGGRQKRALLADGYEAIIAAVYLDGGMEA
                     ARGFIAREFEALIDQVAADANGGGDHKSALQEALQGRGDPLPEYVVTAEEGPAHRRRF
                     RIEVRLRGETLAEADGRTKKEAEQEAARLALERLTPRA"
     gene            complement(3478..4557)
                     /locus_tag="PLT35_04450"
     CDS             complement(3478..4557)
                     /locus_tag="PLT35_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOG28509.1"
                     /translation="MDTPIQDVDARLAAALADEPSRIPVLLGPCGSGRTRALRRLRDR
                     LPAHTAQYVDVERVVSTPERFLERLTAGTPFVWQPPPGAPDGPREAYARVLSYFARAR
                     TSEGGPATFLLDEAFDVRVFESFPGLAGALGHTLAALASSPNRAVLATKFEARALRAL
                     RGASDRFLVLHAPAVAVSAIAASLMATPGARPDWAQDTARAVVALADGRAAYAAAMVR
                     VVSAEPAGACDPAAALARLLGPGGDLACRCRFSFECRLHRARGYGALRAVLEILADDE
                     PLNLTEISLRLGRSPGSTKDYLGWLEDVDLVRVQGKRYRVADPILRLWIGLNTGPDAP
                     AEADAADAVRRYAQARLSAGVTPEA"
     gene            4738..6039
                     /locus_tag="PLT35_04455"
     CDS             4738..6039
                     /locus_tag="PLT35_04455"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOG28510.1"
                     /translation="MNIAVVGAQWGDEGKGKIVDLLARRFSIVARYQGGHNAGHTVHV
                     GGRRFVLRLIPSGILHQGVTCVIGNGVVVDLAALFDEVDGLAGAGIGVDGRLLISDRA
                     HVILPYHPDLDRLREASLGPRMIGTTSRGIGPAYEDKAGRRGLRVCDLADAAALEEHI
                     RDGLARRNELARDGRLAWTDVFDRTRQLGERLKPWIADVSAVLCAAMADGRSILFEGA
                     QGTMLDLDHGTYPYVTSSNATVGGACTGLGIGPRSIHVVLGIAKAYTTRVGEGPFPSE
                     LHGGDGDALRAKGGEFGTVTGRPRRCGWFDAVQMRHAVRVNGFDALAVTKLDVLDGFE
                     RVPVCVGYRLDGEAVSAFPADLGRLRRCTPVLESCPGWNRPTSGVQRYDDLPANAQAY
                     LQRLEDLAGVPVVFASTGAAREDIVVKNAPLAAAWFGSELA"
     gene            complement(6041..6691)
                     /locus_tag="PLT35_04460"
     CDS             complement(6041..6691)
                     /locus_tag="PLT35_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28511.1"
                     /translation="MADGFTILIGPRSLALTATGYPAAKGTELVCFPETEVCAAVEQI
                     VARKPRVVVLPEKLAFSPRGTALTHRLSIDAALARTELVVIDASGRLTPMPPGSQTTW
                     LPLDGAGTRRVPRIRIRDGHDVLVDGAPAALVNLSTLGAQVVSQVALKPRQRVRIVLG
                     AQPAAIKAAGTVIWASFEIPKGTQAPQYRAGLEFVEADPEPLLQLCLQHAAERAAP"
     gene            complement(6700..7341)
                     /locus_tag="PLT35_04465"
     CDS             complement(6700..7341)
                     /locus_tag="PLT35_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28512.1"
                     /translation="MFGGKGHVDDSALVRRYLADRGLEALKPKDEAVVRHLGRCAACS
                     SRYAALRAGLDETRDAVFAEVDAVCSPDRLERQRERILRHVDDGRSGATRVVVFPAPP
                     HRAKPAHEHRVLARWVAAAAVVGLMVGLTAGRLFDVGPGGPLPGAQRQAAAEPASARG
                     VPVIRPAASGLDAEDEFLSELELATSVSAVAELRAIYAFTLEEPNDAPRPGKD"
     gene            complement(7328..>7715)
                     /locus_tag="PLT35_04470"
     CDS             complement(7328..>7715)
                     /locus_tag="PLT35_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG28513.1"
                     /translation="DLPFDAWFTRILANTCRDRQKARRRRERWEVIGFDRRPELGSVV
                     ETARAGGLTPEESVLGGERQRELLKAIDALPKQQRTVLLLCHVEGQSPREVGALLGLN
                     ESTVRVHLFRALRKLRGVLEDSRVRR"
BASE COUNT         1060 a         2703 c         2842 g         1110 t
ORIGIN      
        1 tgtacatagg aaaagctatg gagtcggccc cgatcacccg cgacgatctc gacgcttcgg
       61 cagtcgtcgt cgtgctgacc acgctgcccg ccggcgccga cgcgctcgcc ttcgcgcgcg
      121 ccgtcgtcga cgagcgcctc gcagcgtgcg tgggcgtgct gagcgagatg cagtcggtct
      181 accggtggca gggcgccgtc gagcacgcac ccgagcgcca gctcgtcatc aagacgacgc
      241 gcgggcaggt ggcgcgcctg acggactggc tcgccgcgca tcatccgtac gaggtccccg
      301 agctcatcgt gatgccggcc gacggcggcg atgcctacct tgcctgggtg cgtcagagca
      361 cggcaggctg atctatcatc ctccgtaccg ccgatggcat cgacgctgac cttctccaac
      421 ccggcgggcc ggctcggcgc ctgggcggcc gcagccaggc gattccttct gacgacggtc
      481 gagggccgtc tgatcgtcgg cggcgccgcc gtcaaggtgg cggtgaacgg aatcgctttc
      541 gcggggctgg gcgcgtccag gatcctcgag ggcatcagca ccgccggggg gctggcaatc
      601 ctggcgggcg tcctggggct gctgtaccgc gcctggcgcg gggtgcgcca ccagctgctc
      661 tggcgtgtgc gccggaagct catcatctcc tacgttttca tcggcgtcgt gccggtcatc
      721 ctgatcatcg cgttcttcgt cctcgcgtgg ctgcttctct tcgccaatat cagctcgtac
      781 ctcatccgca gcgcgctcgt ggatctgggc gagggcgcga tgtcggctgc gcgcctcgcg
      841 gccgtcgaga tcgaggatcg cgcggacccg gcgcgggtgt ccgcgatcct ggaacggcgg
      901 gcggccgccg aggccgtccg gcacgccggg atctccctgg ccgtcgtgcc catcgacgcc
      961 gaccggtgtc ccgcgcccgc tgacggagcg ccggctcgcg cggtcgcggc cgcctcagca
     1021 ggctcgtggg cgcacacgct ggctccgggt ctggttccag cgtgggttcc gtgcgacggg
     1081 ttcgctggcg tcctggccta cggagcaagg cccggccccg aggcgcccgc cgccgaggtg
     1141 ttcgtgcggg ccgtcgcgtt ccccgagcgc tcgcggggat tcgtcgtcat cgccgacgtg
     1201 cccgtttcac ggccggtgaa ggccgcgatc gaagcggaga cgggcatcgt catcggcgag
     1261 gcgagcctgg tggggtccac gtcgctgctc gtccgaggca cagccggcac cccggccggg
     1321 gacctcccgc aggcgtccac cgccgccgag cgcgtctggc ggtttccgtg ggtcgcgctc
     1381 ctcgaccacg tggactggac gacggggcgg accgggccgc tgccaatcaa catcggcctc
     1441 agcgtccgcg cgatctatca acggctctcc gcctcgcagg cctcgctcgg cggcactcgg
     1501 agcttcggcg atctgctcct cgcgctgctg ctgttcgtca gcgggctctt cttcatcatc
     1561 gaggtcgtcg cgctcgtgat ggggctggcg ctggcgcggt cgatcaccgg cgcgatccac
     1621 gagctcttcg caggcaccga gcgcgtgcgc cagggcgact tcgggcaccg gattcccgtg
     1681 ctggcccggg accaactggg cgagctggcc agctcattca accagatgac cggcagcatc
     1741 gaggatctgc tccggcaggc cgccgagaag aagcgactcg aggaggagat gcgcctcgcg
     1801 cgcgagatcc agatgtcgct cctgccgcgc gggccgctcg tcgtgccggg agtggccatc
     1861 agcgcgctct gcgtgccggc ccgggaggtt ggcggcgact actacgacgt cctgtcgctg
     1921 cccgacgggc ggttcggcgt gctgattgcc gacgtgtccg gcaagggcat gtccgcggcg
     1981 ctgtacatgg cggagctgaa agggctcatc ctctcgctca gccaggtgca tctctcgccg
     2041 cgcgagctcc tgattggcgc caaccgcctg atctcgatgc acctggacag ccgcagcttc
     2101 atcacgatga cctacgcggt catcgatccg gccgcgggcg tgatgaccta cgcgcgcgcg
     2161 ggccacacgc cgctgttgta cttgccggga cgaggcgccg acggcagggc caggacgctc
     2221 gcgcccgacg gcatgatcgt gggcctgcgg ctcgacgacg gcgagcgctt caagtccctg
     2281 ctggtggagt cgacgctgcg gctggcggcc ggcgacctcc tggtgttctt caccgacggc
     2341 gtcagcgagg cgatgaacga ccgcgccgac tgcttcggcg aggcccagct ggccgaactc
     2401 gtcgagcagc acgcccatct cccgattggc gagctgcggg agcgcattct ccgggagatt
     2461 acagccttcg tcggcgaggc gccccagcac gacgacatga ccctgatttt gttgaaggtc
     2521 cggggcgacg agcaggccgc tggggcgtca tgaccgatcc cgtcgtcgtc cttcgcacgc
     2581 actccgacat cgaggccagc atcgtcatcg ggctgctcga cgcccacggc atcccggcga
     2641 gcctggcctc ggacgtgccg cacgcggttt tccccctgac gatcaacggg ctcggggagg
     2701 tgcgggtgtc ggtgcccggg aaccgggccg aggaggcgtc gcgtatcctg gcggattacc
     2761 ggcgcgccgg cggagcggag cgcgcggtga agatcggcga ggaattcgag gccctcgaag
     2821 ccgcaatcgg gcatcgtttc aaggaccgcg gcaccctcga gcacgccctg acgcaccggt
     2881 cgcgcgcaaa cgaggatgtg acgggggggg tgttcgacaa cgagtccctc gagttcctgg
     2941 gcgatgcggt gctcgggttc gtcatcgccg agaccctgtt cgtgcggttc ccggatcggg
     3001 acgagggcaa gaagtcgaag atgaaggcgg cgctcgtgtc gacccagacg ctcgcgggcc
     3061 aggccgagcg cctgggcctc ggcgcgcacc tgctgctcgg ccgcggcgag gagaagaccg
     3121 ggggtcggca gaagcgcgcg ctcctggccg acggctacga ggcgatcatc gcggccgttt
     3181 acctcgacgg cggcatggag gccgcccgcg ggttcatcgc gcgcgagttc gaggcgctca
     3241 tcgaccaggt agccgccgac gccaatgggg gcggtgatca caagtcggcg ctgcaggagg
     3301 cgctgcaggg gcgaggcgac ccgctccccg agtacgtggt gactgcggaa gaaggcccgg
     3361 cccaccggcg ccgcttcaga atcgaggtcc ggctccgggg ggaaaccctc gccgaggcgg
     3421 acggccggac gaagaaggaa gcggagcagg aggccgcgag gctggcgctc gagcggctca
     3481 cgcctcgggc gtgacgccgg ctgacagcct ggcctgcgcg taacgccgga ccgcgtcggc
     3541 ggcgtccgcc tcagccggcg cgtcggggcc cgtgttcaac ccgatccaga ggcgcaggat
     3601 cgggtctgcg acgcgatagc gcttgccctg aacgcgcacg agatcgacgt cctccagcca
     3661 ccccaggtag tccttggtcg agccgggcga ccggccgagg cgaagcgaga tctccgtcag
     3721 gttcaggggc tcgtcatcgg cgaggatctc gaggacggcc ctgagcgctc cgtagcctcg
     3781 cgcccggtgg agccggcact cgaagctgaa ccggcagcgg cacgcgagat cgccgccggg
     3841 cccgagcagg cgggccaggg ccgcggcggg gtcgcatgcg cccgccggtt cggcggacac
     3901 gacacggacc atcgcggcgg cgtacgccgc ccggccgtcg gcgagcgcca ccaccgcgcg
     3961 cgcggtgtcc tgcgcccagt ccgggcgcgc ccctggcgtc gccatgaggc tggcggcaat
     4021 tgccgagacc gcgacggcag gcgcgtgcag cacgaggaag cgatcggaag ccccgcggag
     4081 cgcgcgcagg gcccgcgcct cgaatttcgt ggccagcacg gcgcggttgg ggctcgacgc
     4141 gagggcggcc agcgtgtggc ccagcgcgcc ggccagcccg gggaagctct cgaacacacg
     4201 cacgtcgaag gcctcgtcga gcaggaacgt ggccgggccg ccctcggagg tccgcgcccg
     4261 cgcgaagtac gagagcaccc gcgcgtacgc ctcacgaggg ccgtcaggag cgcccggagg
     4321 cggctgccag acgaacggcg tccccgcggt caggcgttcg agaaagcgct cgggcgtgga
     4381 gacgacgcgc tcaacgtcca cgtactgggc cgtgtgcgcc gggaggcggt ctcgcaggcg
     4441 gcggagggcc cgcgtgcggc ccgagccgca gggaccgagc agcaccggaa tccgggacgg
     4501 ctcgtcggcg agggccgccg cgagccgggc gtcgacgtcc tgaatcggag tgtccatgcg
     4561 cggctgacgg ggaacgactc ggcgcgcgcg aggcgccggg ccgtagatgc tcatgcacca
     4621 tacgagggcc tctccgactt gtcaacctcg cgccccggtg ttccggcgcc ctgtgctaac
     4681 atcccgcgtg gcccgcgggc acgagcgccc gcgtgagcgc tcggagcgga cctgtcgatg
     4741 aacatcgccg tcgtgggcgc gcagtgggga gacgagggca agggcaagat cgtggacctg
     4801 ctcgcgcgga ggttctcgat tgtggctcgc tatcagggcg ggcacaacgc cggccacacg
     4861 gtgcacgtcg gcggacgccg cttcgtgctc cggctgatcc cgtcgggcat cctgcaccag
     4921 ggcgtgacgt gcgtcatcgg gaacggcgtg gtcgtcgacc tggcggcgct cttcgacgag
     4981 gtggatggcc ttgccggcgc gggcatcggc gtggacggcc gcctgctgat ctccgaccgg
     5041 gcgcacgtga tcctgcccta ccatcccgac ctcgatcggc tgcgcgaggc cagcctgggc
     5101 cctcgcatga tcggcacgac ttcgcgcggc atcggccccg cgtacgagga caaggccggc
     5161 cgccgggggc tgcgcgtctg cgacctcgcg gacgcggccg cgctcgagga gcacatccgc
     5221 gacgggctgg cccggcggaa cgagctggct cgcgatgggc gcttggcgtg gactgacgtg
     5281 ttcgatcgca cgcggcagct cggcgagcgc ctgaagccgt ggatcgcgga tgtctccgcg
     5341 gttctctgtg ccgcgatggc cgacggccgg tcgattctct tcgaaggggc ccaggggacg
     5401 atgctcgatc tcgaccatgg gacatacccc tacgtcacct cgtcgaacgc gacggtcggg
     5461 ggcgcctgca ccgggctcgg catcgggccg cgatccatcc acgtcgtgct cggcatcgcg
     5521 aaagcctaca ccacgcgcgt gggcgagggg ccgttcccca gcgaactgca cggcggagac
     5581 ggcgacgcgc tgcgggcgaa gggaggcgaa ttcggcacgg tgacgggccg tccgcggcgc
     5641 tgcggctggt tcgacgccgt ccagatgcgg catgctgtcc gggtcaacgg cttcgatgcg
     5701 ctcgcggtga cgaagctgga cgtgcttgac ggcttcgagc gcgtgcccgt ctgcgtcggc
     5761 taccggctcg atggcgaggc ggtgtcggcg tttccagccg acctcggccg gctgcggcgc
     5821 tgtacgccgg tgctcgagtc gtgtcccggg tggaaccggc cgaccagcgg cgtccagcgc
     5881 tacgacgatc tgccggcgaa cgcgcaggcg tacctgcagc gactcgagga tcttgcgggg
     5941 gtgccggtgg tgttcgcctc gacgggcgcg gcgcgcgaag atatcgtcgt gaagaacgcg
     6001 ccgcttgcgg ccgcgtggtt cggctccgag ctggcgtaga tcagggggcc gcgcgctcgg
     6061 cggcgtgttg caggcaaagc tggagcagcg gctccggatc ggcctccacg aactccagcc
     6121 cggcgcggta ctgcggcgcc tgcgtgccct tgggaatctc gaacgacgcc cagatcaccg
     6181 tgccggcggc cttgatggcg gcgggctgcg cgccgaggac gatgcgcacg cgctggcgcg
     6241 gcttcagcgc gacctgcgac accacttgcg caccgagggt ggacaggttc acgagcgcgg
     6301 cgggtgcgcc atcgacgagc acgtcgtggc cgtcgcggat gcggatccgc ggcacgcgcc
     6361 gcgtgcccgc gccgtcgagg ggcaaccacg tcgtctggct cccgggcggc atcggggtca
     6421 accggcccga cgcgtcgatg acgaccagct cggtcctggc gagcgccgcg tcgatggaga
     6481 ggcggtgggt gagcgccgtg cctcgggggc tgaacgcgag cttctcgggc agcacgacca
     6541 cccgtggctt gcgagcgacg atctgctcga cggccgcgca aacctcggtc tccgggaagc
     6601 acaccagctc ggtgcccttc gccgcggggt atccggtcgc cgtcagggcc agcgaccggg
     6661 gcccgatcag gatcgtgaag ccgtccgcca tccgggaggt cagtccttgc ccgggcgcgg
     6721 cgcgtcgttc ggctcttcca gcgtgaacgc gtagatcgcg cgcagctcgg cgaccgcgga
     6781 cacggacgtc gccagttcga gttcggacaa gaactcgtct tcggcgtcca gcccggaagc
     6841 ggccggccgg atcaccggca cgcctcgggc gctggccggc tcggctgccg cctgccgctg
     6901 cgcgccgggc agggggccgc ccgggccgac gtcgaacagg cggccggccg tgaggcccac
     6961 catgagaccc acgaccgccg cggccgcgac ccaccgcgcc aggacgcggt gttcgtgcgc
     7021 cggcttggcg cgatgcgggg gcgcggggaa cacgaccact ctcgtcgcac cggaccggcc
     7081 gtcgtcgacg tgccgcagaa tccgctcgcg ctggcgctcg aggcggtcgg gcgagcacac
     7141 cgcgtcgacc tccgcgaaga cggcgtcgcg cgtctcgtcg agcccggcgc gcagcgcggc
     7201 ataccggctg ctgcaggccg cgcagcggcc gaggtgcctc accaccgcct cgtccttcgg
     7261 cttgagcgct tcgaggcccc tgtccgccag ataacggcga acgagcgcgc tgtcgtcgac
     7321 gtgtccttta ccgccgaaca cggctgtcct ccaggacgcc ccgcagcttg cggagcgcgc
     7381 gaaacaagtg cacccgcacc gtcgactcgt tcaggccgag caacgcgccg acctcgcggg
     7441 gagactggcc ctccacgtgg cacagcagca ggaccgtccg ctgctgcttc gggagcgcgt
     7501 cgatcgcctt cagcagctcc cgctgccgtt cgcctcccag caccgattct tccggcgtca
     7561 gcccgccggc ccgagcggtt tccacgaccg agcccagctc ggggcgccga tcgaagccga
     7621 tgacttccca tcgttcgcgg cggcgcctgg ccttctggcg gtcccggcag gtgttcgcga
     7681 ggatccgggt gaaccacgcg tcgaacggca gatcc
//
LOCUS       DAOSIF010000086         7709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8730_length_7709_cov_4.211654, whole genome shotgun sequence.
ACCESSION   DAOSIF010000086 DAOSIF010000000
VERSION     DAOSIF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8730_length_7709_cov_4.211654"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLT35_04475"
     CDS             complement(<1..294)
                     /locus_tag="PLT35_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28514.1"
                     /translation="MRRAALAAWLLLPLASCRSAAHELTAGPGGRDAPRAFVEALADR
                     FGPIDRDPAYDALRRKLNRMSFAPSRGFDDPAAWTAKGPGWREVDLVGRGAGGA"
     gene            complement(341..469)
                     /locus_tag="PLT35_04480"
     CDS             complement(341..469)
                     /locus_tag="PLT35_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28515.1"
                     /translation="MRRPLDWRRESEPAGTAATTGAAATALRRRLRFALPAAGGGR"
     gene            complement(561..1232)
                     /locus_tag="PLT35_04485"
     CDS             complement(561..1232)
                     /locus_tag="PLT35_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG28516.1"
                     /translation="MRTHAKILVIDIGGNNVKLLASGHKEARKFPSGPKLTPRKMLAG
                     VRALTKDWPYDAVSIGYPGVVHKNRITEEPINLGKGWVGFNFRAAFRKPVKVVNDAVM
                     QAIGSYKGGTMLFLGLGTGLGTALIVNGHVVPMELGHFPYRKMTIEDYLGTRGVRIFG
                     VTRWRKLVESVIGEFVSAFLVDEVVLGGGNVKKLTRMPKGCRAGSNSNAFLGGFRLWD
                     RGGPR"
     gene            1354..2151
                     /locus_tag="PLT35_04490"
     CDS             1354..2151
                     /locus_tag="PLT35_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG28517.1"
                     /translation="MDAPQRLQELAYRIPYHYLPVAEGGRFSQHEYWSWGYRYLGRLE
                     VALSLLGQHPFASLLDIGCGDGRFLREVRRRFARPRLLGIDVSENAVRWARALNPGLE
                     FQTLDLLERRLDEQFDAVTLLEVVEHVSPERLPDVLRAAAAHLRPGGRLVLTTPHTNM
                     RLDPRHFQHFDRARLCALLEPDFDRISCTPFDYVDWPTRALLQMLGGSGRFFLVTHAG
                     LNAWFFRRYMKRRLYGSGESGCQRLAVAARKRAAPPDAGGGPAAGTD"
     gene            2159..3271
                     /locus_tag="PLT35_04495"
     CDS             2159..3271
                     /locus_tag="PLT35_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG28518.1"
                     /translation="MVSLRHRFGFLRGLMSGGRARGLPLYANVDVTHRCNLACAYCRW
                     HSRLLPETLLDGGLDLDLSPEAFRRFCEGARGTGTQTLQFVGAGEPLLHPEIATLVRT
                     AKEHGYRVLLYSNGTTLDERSVRMLVEARLDVLRVTLGDTTSEQFDRKHPHVKPGLFP
                     RILEGLARVTRVKREAGSAVPRLELGVPIDRDLVADLDAIVGIAASTGCDGLWFSVVL
                     DFGRDELAPFCLGPDEVERACDALRRARGRIERLSLAHNIDDVLVRYRLGKDVWKQAP
                     CYSAWYFAFLRSDGRLTVCQRGQVPMGDLREQSFGEIWNGEAYREFRRRALTDPSPPY
                     DCEFCPHAVNNHRVHRYARLVLPLLGRRRPPAGPGA"
     gene            3315..4433
                     /locus_tag="PLT35_04500"
     CDS             3315..4433
                     /locus_tag="PLT35_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG28519.1"
                     /translation="MPLLRDLVIKPTLACTANCPTCALRRHLHADLKGSGTLPFERWR
                     DLLAEAAALGASRVTISGGEPTLYPRLAGLVEAGRRHGCEVRVNSNGSLITEPYAKQL
                     LDAGLSVMCVSLYASTGAKMADMRGNATLWGKAAEAIRIFAALEAEYPGFEIRSQTIL
                     SRDNLDDLPALLDLHARLGSRHVTVSYLEGDSEGQHLPRAADVARFRQAVLPDAVRVC
                     RARRGPAGLLAARSVGRLFSPAILAAGDWSSGRYWSRERGCRVPSYQALILANGDVHP
                     CNIVEYTHEPVVGNVSRESLTGVWTGEAWREFRAARHPGCRFCPINRHTGVFLRPRSL
                     IEALAGTIFYDRAHADRARRVVEAWTALRRGARRARTA"
     gene            complement(4486..5907)
                     /gene="sthA"
                     /locus_tag="PLT35_04505"
     CDS             complement(4486..5907)
                     /gene="sthA"
                     /locus_tag="PLT35_04505"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HOG28520.1"
                     /translation="MNTFDVVVIGGGPGGERAAIQAARAGKRVALVERENVVGGMRVN
                     WGTIPSKTLRESAVFVHGLRHNRLDGIKVEIADTITIPELKYREQRVVQRELELILSS
                     LTRYAIEIVRGEGRFVDPHTVAVHGADGRMRLRLRGDVIVIAVGSSPNRPADVRFDDE
                     TVFDSTTVLRLPRMPQSMIVLGAGVIGVEYASIFSALGVAVTLVDTRADLLPFVDREI
                     ASLLRRELQRLGTVFVPNDQYAAVERIDGAPPAVRVTTRSGNVLEADALLYCVGRDGN
                     TRGIGLEAIGITPDPRGLIKVNEHFQTAHPHIYAVGDVVGYPALASTSMEQGRQAIRH
                     AFGIPGPRLRTERLPFAIYAIPEVSYIGETEEALRQQGLPYVVGRGHYEMNPRGQIVG
                     ASGGLVKLLFARETMRLAGVHIIGHTASELIHTGQAFLDSGADATHIAETLYNYPTFS
                     DMYRHAALVALRAAADDAPPPAA"
     gene            6358..7626
                     /locus_tag="PLT35_04510"
     CDS             6358..7626
                     /locus_tag="PLT35_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOG28521.1"
                     /translation="MVTAMKKRRVDVPSAGSRAASQDGGRGLRAAGVPCRVYGTDLEQ
                     GARQQMENACRLPVAVAGALMPDAHQGYGLPIGGVLATDNAVIPYAVGVDIACRMKMT
                     VLDLPASTLATGRYELRRVIERETRFGVGAEFRDRRDHPVMDRDWNVTMVTGRLRDKA
                     WAQLGTSGSGNHFVEFGVLETFEALGGLEPGEHLALLSHSGSRGTGAAVARHYSKLAR
                     DLRPGLPQEFGHLAWLDLESEAGMEYWAAMELMGAYAAANHELIHRHVARALGASVRF
                     DVENHHNFAWRERHVLPDGSEREVIVHRKGATPADTGVLGIIPGSMAAPGYVVRGKGS
                     AESLRSAAHGAGRRMSRTRARQAFTWDDVRRVLDARGVTLLSAGLDEAPMAYKDIDAV
                     MAAQRSLIHPVARFTPRLVKMAPAGEAPED"
BASE COUNT         1021 a         2818 c         2752 g         1118 t
ORIGIN      
        1 cgcgcccccg gcgccgcggc ccaccaagtc gacctcgcgc cagccgggcc ccttcgccgt
       61 ccacgcggcc ggatcgtcga acccgcgcga cggcgcgaaa gacatcctgt tcagcttccg
      121 gcggagggcg tcgtaggcgg ggtcgcggtc gatgggcccg aagcggtctg cgagcgcctc
      181 gacgaacgcg cgcggggcgt cccggccgcc gggccccgcc gtcagctcgt gcgcggcgct
      241 ccggcaggag gccagcggca gcaggagcca ggcggcaagc gcggcgcggc gcatgggatc
      301 atcatcccac ggtctcagcg gatcgaggcc ggcgcgagcg tcaccgcccg ccgccggcgg
      361 cgggaagcgc gaagcgcagg cgccgcctca gcgccgtcgc cgccgcgccg gtcgtcgcgg
      421 ccgtgccggc cggctcgctc tctcggcgcc agtcgagcgg gcggcgcatg ggacgggcaa
      481 tccggcgggg accatgcaac cgccggaagg gccggacgcc gcggacgtcc cgcctgctgc
      541 gccggcctgg cagctcgggc tcagcgcggg ccgccgcggt cccacaggcg gaacccgccg
      601 aggaacgcgt tcgagttcga cccggcgcgg cagcctttcg gcatgcgggt gagtttcttg
      661 acgttcccgc cgccgagcac cacctcgtcc accaggaacg ccgagacgaa ttccccgatg
      721 accgactcca ccagcttccg ccagcgggtc acgccgaaga tcctgacgcc ccgggtcccg
      781 aggtagtcct cgatcgtcat cttcctgtac gggaagtggc cgagctccat cggcacgacg
      841 tggccgttga cgatgagcgc ggtgcccagg cccgtgccga ggccgagaaa gagcatcgtg
      901 ccgcccttgt agctgccgat ggcctgcatc acggcatcgt tgaccacctt gacgggcttg
      961 cggaaggcgg cgcggaagtt gaacccgacc catcccttgc cgaggttgat cggctcctcg
     1021 gtgatccggt tcttgtgcac gacgccgggg tagccgatcg aaaccgcgtc gtacggccag
     1081 tccttcgtga gcgcccggac gccggccagc atcttccgcg gcgtgagctt cgggccggac
     1141 gggaacttcc gcgcctcctt gtgcccgctc gcgaggagct tgacgttgtt cccgccgatg
     1201 tcgatgacca ggatcttcgc gtgcgtccgc atccggcctc cccttgtggc tgcgctcggc
     1261 gcccgtctga agcagtgtcg cacaagtgcg gccggggcgg cgtcgcgagc cggctgcgct
     1321 atgatgatgg ggctccaagc cgcgatcgcc accatggacg cgccgcagag gcttcaggaa
     1381 ctcgcctatc gcatccccta ccactacctg ccggtggcgg aaggcggccg cttctcccag
     1441 cacgagtact ggtcctgggg ataccgctat ctgggacggc tggaggtcgc cctctcgctg
     1501 ctgggacagc atcccttcgc gtcgctcctc gacatcggct gcggcgacgg ccggttcctg
     1561 cgcgaggtcc ggcgccgctt cgcacgtccc cgcctgctgg gcatcgacgt gtcggagaac
     1621 gccgtccgct gggcgcgggc gctcaacccg ggcctggagt tccagacgct cgatctcctg
     1681 gagcgccggc tcgacgagca gttcgacgcg gtcacgctgc tcgaggtcgt cgagcacgtc
     1741 tcgcccgaga gactcccgga cgtcctgcgc gcggcggccg ctcatctccg ccctggcggg
     1801 cggctggtcc tcaccactcc ccacacgaac atgcgcctcg atccgcgcca cttccagcac
     1861 ttcgaccgcg cgcgcctttg cgcgctgctc gagcccgact tcgatcggat ctcctgcacg
     1921 cccttcgact acgtggactg gccgacgcgg gcgctcctgc agatgcttgg cgggtcgggc
     1981 cggttctttc tcgtcaccca cgcgggcctc aatgcctggt tcttccggcg gtacatgaag
     2041 cggcgcctct acggatcggg cgaatcgggc tgccagcggc tggcggtggc cgcgcgcaag
     2101 cgcgccgccc cgccggacgc cggcggcggt cccgccgcag ggaccgactg aggtcgtcat
     2161 ggtctctctg cgtcacaggt tcggcttcct gcgcgggctg atgtcgggag gccgcgcccg
     2221 cggcctgccg ctgtacgcga atgtcgacgt gacgcaccgc tgcaacctgg cctgcgccta
     2281 ctgccgctgg cattcccgcc tgcttcccga gacgctgctc gacggcggcc tcgacctcga
     2341 tctgtcaccg gaggcgttcc ggcggttctg cgagggcgcc aggggcaccg ggacgcagac
     2401 cctgcagttc gtcggggcgg gagagccgct gctccacccc gaaatcgcga cgctggtcag
     2461 gacggcgaag gagcacggct accgggtcct gctctactcg aacggcacca cgctggacga
     2521 gcggtccgtc cggatgctcg tcgaggccag gctcgatgtg ctcagggtca cgctcggtga
     2581 caccacgtca gagcagttcg atcgcaagca tccccacgtc aagcccggtc tgttcccgcg
     2641 gatcctcgaa ggcctggcgc gggtcacgcg cgtcaagcgc gaggcgggct cggcggtccc
     2701 gcgcctcgaa ctgggcgtgc ccatcgaccg ggatctcgtc gcggatctgg acgcgatcgt
     2761 cgggatcgcc gcctccacgg gctgtgacgg cctctggttc tcggtcgtgc tggatttcgg
     2821 ccgggacgaa ctggcgccgt tctgcctcgg tcccgacgag gtcgagcgcg cgtgcgacgc
     2881 ccttcggcgc gcgcgcgggc gcatcgagcg gctgtcgctg gcgcataaca tcgacgatgt
     2941 cctcgtccgc taccgcctcg ggaaagacgt ctggaaacag gctccctgct acagcgcgtg
     3001 gtacttcgcg ttcctgcgca gcgacggtcg gctgacggtg tgccagcggg gccaggtgcc
     3061 catgggggat ctcagggagc agtccttcgg cgagatctgg aacggcgagg cgtaccgcga
     3121 gttccgccgc cgcgcgctca ccgacccgtc cccgccctac gactgcgagt tctgtcccca
     3181 cgcggtgaac aaccaccgcg tccaccggta cgcgcggctg gtgcttccgc ttctcgggcg
     3241 ccgccggccg cccgccggcc caggggcatg atgaccacgg ccgcccggcg ccggctggac
     3301 tcgcgatgac cccgatgccc ctccttcgcg acctcgtcat caagcccacg ctggcgtgca
     3361 ccgccaattg cccgacctgc gcgctgcggc gccacctgca cgccgacctg aagggatccg
     3421 ggaccctgcc gttcgaacgg tggcgcgatc tcctggcgga ggccgccgcg ctcggcgcga
     3481 gccgcgtgac gatcagcggc ggcgagccca cgctctatcc gcggctggcc gggctcgtcg
     3541 aggcaggcag acggcatggc tgcgaggttc gcgtcaattc gaacggcagc ctgatcaccg
     3601 agccctacgc gaagcagctg ctcgacgccg ggctcagcgt gatgtgcgtc tcgctctatg
     3661 cctcgaccgg cgcgaagatg gcggacatgc gcggcaacgc gacgctgtgg ggcaaggccg
     3721 ccgaggcgat tcggatcttc gcggccctcg aggccgagta cccgggcttc gagatccgca
     3781 gccagacgat cctgagccgc gacaacctcg acgatctccc cgcgctgctc gacctgcacg
     3841 cccgccttgg ctcgcgccac gtcacagtgt cgtacctgga aggagactcc gaggggcagc
     3901 acctcccccg cgcagcggac gtcgcgcgct tccgccaggc ggtcctccct gacgcggtgc
     3961 gggtgtgccg cgcgcggcgt ggccccgcgg ggctgctcgc cgcgcgcagc gtcggccgcc
     4021 tcttctcgcc ggcgatcctc gccgccggcg actggtcgtc ggggcggtat tggagccggg
     4081 agcgcggctg ccgcgttccc tcgtaccagg cgctgatcct ggcaaacggc gacgtgcatc
     4141 cctgcaacat cgtggagtac acgcacgagc cggtggtcgg caacgtctcg cgcgagtcgt
     4201 tgaccggcgt ctggaccggc gaggcctggc gcgagttccg tgcggcgcgg cacccgggct
     4261 gccgcttctg ccccatcaac cgccacacgg gggtctttct caggcccaga tccctgatcg
     4321 aagcgctggc cgggacgatc ttctacgacc gggcgcacgc ggaccgggcg cgccgggttg
     4381 ttgaggcctg gacggcgctc agacgagggg cccggcgagc ccggacggcc tgaagccggc
     4441 gcaggtcagg ctcgcccgga agaggcaggg cctgcccgcg cgccgtcagg cggccggggg
     4501 cggggcgtcg tcggctgcgg ccctgagcgc cacgagcgcc gcgtggcggt acatgtccga
     4561 gaaggtcggg tagttgtaga gcgtctcggc gatgtgcgtc gcgtcggcgc ccgagtcgag
     4621 gaacgcctgc ccggtgtgaa tcagctcgct cgcggtgtgg ccgatgatgt gcacgccggc
     4681 cagccgcatc gtctcgcggg cgaagagcag cttgaccagc ccgccggacg ccccgacgat
     4741 ctggcccctc gggttcatct cgtaatgccc gcgcccgacg acgtacggga ggccctgctg
     4801 ccgcagcgcc tcttcggtct cgccgatgta gctcacctcg gggatggcgt agatcgcgaa
     4861 cgggaggcgc tcggtcctga gccgcggccc tgggatgccg aacgcgtgcc ggatcgcctg
     4921 ccggccctgc tccatcgacg tgctggcgag ggccgggtag cccacgacgt cgccgacggc
     4981 gtagatgtgg gggtgcgcgg tctggaagtg ctcgttcacc ttgatgaggc cgcgcggatc
     5041 gggcgtgatg ccgatggcct cgaggccgat gccgcgggtg ttgccgtccc ggccgacgca
     5101 gtagagcagc gcgtccgcct cgagcacgtt gccgctccgg gtggtcaccc ggacggcggg
     5161 cggcgcgccg tcgatgcgct cgacggccgc atactgatcg ttcggcacga acaccgttcc
     5221 gaggcgctgg agctcgcgcc gcagcaggga ggcgatctcg cggtccacga acgggaggag
     5281 atcggcgcgc gtgtccacga gcgtcaccgc cacgccgagc gccgagaaga tcgacgcgta
     5341 ctcgacgccg atgacgcccg cgcccagcac gatcatcgac tgcggcatcc gcggaaggcg
     5401 cagcacggtc gtgctgtcga acacggtctc gtcgtcgaac cggacgtcgg cggggcggtt
     5461 gggcgacgag ccgacggcga tcacgatgac gtcgcccctc agccgcaggc gcatccggcc
     5521 gtccgcgccg tggaccgcca ccgtgtgcgg atcgacgaag cgcccctcgc cccggacgat
     5581 ctcgatggcg tagcgcgtga gcgacgacag gatgagctcg agctcgcgct gcacgacgcg
     5641 ctgctcccgg tacttcagct cgggaatcgt gatcgtgtcg gcgatctcga ccttgatgcc
     5701 gtccagccgg ttgtggcgaa ggccgtggac gaacacggcg ctctcccgga gcgtcttgct
     5761 cgggatcgtg ccccagttca cgcgcatgcc gccgaccacg ttctcgcgct cgacgagcgc
     5821 gacgcgcttg ccggccctgg cggcctgaat cgcggcccgc tcgccgccgg gcccgccgcc
     5881 gatgacgacg acatcgaagg tgttcacgga cgctcctcgc cagcctggct ggcagacaag
     5941 cgcgcggccg ccgcgatggc gtcagcaggg cggcgttcgc ctgctggacc tgcccgggtc
     6001 cggccgcggc gtccagttcg cctgggcgtc gcccccgccg agtcggcgtg agcgccctag
     6061 tgcgcggcgt gcttgtcccg ccgagcggcc cctcgggctg gcggccccgc cggccgccgt
     6121 cagccgcgtc gggaacacgc gaatcctcgc cggcggctcg tccgtcgcgc ccccgtcgac
     6181 tcgccccggc ccgcgcgcga cgcccggcac ccgcacgccg ccattgtacg tccggccctt
     6241 ccgctcgcga agcggcgcct ccggccccgc gcctgcctcg ggccgcgggg cgcccgtgcc
     6301 ggatgcgggc gggcgggtgc gtgaatccat gacgcggcaa cccgcgaagg gtgcatcatg
     6361 gtcacagcca tgaaaaagag gcgcgttgac gtgccatccg ccgggagccg ggcggccagc
     6421 caggacgggg gccgcggcct tcgcgccgcc ggcgtcccct gccgggtgta cgggacggac
     6481 ctggagcagg gcgcccggca gcaaatggag aacgcctgcc gcctgcccgt ggccgttgcg
     6541 ggcgccctga tgcccgacgc gcaccagggg tacggcctgc ccatcggcgg cgtgctcgcc
     6601 acggacaacg ccgtcatccc gtacgccgtc ggcgtggaca tcgcgtgccg gatgaagatg
     6661 acggtgctcg acctgcccgc gtccaccctt gcgacgggcc gctacgagct ccggcgggtg
     6721 atcgagcgcg agacgcggtt cggcgtcggg gcggagttcc gcgaccggcg cgatcatccg
     6781 gtgatggacc gcgactggaa cgtcacgatg gtcaccggtc gcctcaggga caaggcctgg
     6841 gcccagctcg ggacgagcgg cagcggcaat cacttcgtcg agttcggcgt gcttgagacg
     6901 ttcgaagcac tcggcgggct cgagcccggc gagcacctcg ccctgctcag ccactcgggc
     6961 agccgcggca cgggcgcggc cgtggcgcgg cactacagca agctggcgcg tgatctccgc
     7021 ccggggctgc cccaggagtt cggccacctc gcgtggctcg acctggagtc ggaggccggc
     7081 atggagtact gggcggcgat ggagctgatg ggcgcctacg ccgcggcgaa ccacgagctc
     7141 atccaccgtc acgtcgcccg cgccctcggc gcctcggtcc gcttcgacgt cgagaaccac
     7201 cacaacttcg cctggcggga gcgccacgtg ctcccggacg gctccgagcg ggaggtcatc
     7261 gtgcaccgca agggtgcgac gcccgcggac acgggcgttc tcggcatcat ccccgggtcg
     7321 atggcggcgc ccggctacgt ggtgcgcggc aagggctcgg ccgagtcgct ccgctcggcg
     7381 gcgcacggcg cgggccggcg catgagccgc acgcgcgcca ggcaggcgtt cacgtgggac
     7441 gatgtcaggc gcgtgctcga cgcgagaggc gtgacgctcc tgtcggcggg cctggacgaa
     7501 gcgccgatgg cgtacaagga catcgacgcc gtcatggcgg cccagcgaag cctgatccac
     7561 ccggtggccc ggttcacgcc ccgcctggtc aagatggccc cggccggcga ggcgcccgag
     7621 gactgagtcg catcgtcccg gaggagcggc gcgcgccagg ccatgcttcc ggctgtcagc
     7681 gcggccggct tcgccttcca cggccggtg
//
LOCUS       DAOSIF010000087         7697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8745_length_7697_cov_3.188563, whole genome shotgun sequence.
ACCESSION   DAOSIF010000087 DAOSIF010000000
VERSION     DAOSIF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8745_length_7697_cov_3.188563"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /locus_tag="PLT35_04515"
     CDS             <1..2142
                     /locus_tag="PLT35_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28522.1"
                     /translation="GLPLIAAPFFAVGGYPAVVLLMILLAAAASGLAWTWARRITGSV
                     SSATFAWAATTLSLPFLANAGTVFPEVPAALAVLAAVTAAFREEASGNASIASAGCSA
                     GPWRALAVGLAAAALPWLHAKYAPLSAVLIAVGARRLWRDRPADGRAGTRLMVVAAAP
                     YAASLAGWMAFHYAVWGSPWPSAPYGGASGTQMSAWTWIRGVPGLLADQEYGVLAYAP
                     ALAIAFLGVRSMWRAGGRARGVATDLSLLVAAMLATVGAFQMWWGGSSLPGRMLVSVI
                     PLLAVPVAWEFRASAERPERRAAYRLLLIVGFALALAAVLARGGASLALTRNGISRLI
                     EWLSPDWHLWAYAPDFIMQPAWVAWLQLAIWAACILACGWAVGTLSARRSAARGASRT
                     GRGRAFLRADAGAFFALVLVTLLMPPALGSRLKENPAPEERTRIALLESFDPHARPIG
                     VRYDPLSLVDAASLPGVFTLSARPGSRTARQPAPVLLNARFALPSGRYEVEIASRSPA
                     ALAGSLALQAGRSGGSLQEWSVAAGPGERWRATFDLPVDINYVGFRASDGLAPQVGEL
                     RIRPLRVVTNLDRVAASEVLASSAFSHYVFLFHDGAAYPEPAGFWVRGAGRAVVSVVS
                     RTGRLATNSRMRLRLLSLIPNAVSVETPDRTWTVELEAGVASEIEIQPTVLDGTLRMA
                     LRPASGFRPSERTPDNPDRRFLGCWVELAEL"
     gene            2200..2694
                     /locus_tag="PLT35_04520"
     CDS             2200..2694
                     /locus_tag="PLT35_04520"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOG28523.1"
                     /translation="MALVDALLAEYDHELAVTRKVLERVDEARLGWQPHERSMSLGRL
                     AKHVAEVPRWARTILDDAEFDLADEADGPAASESLSDLLTAFDGLVLGVRARLAAKTD
                     AELATAWTLKRNGQDLFSMPRSAAWRTLIMNHLIHHRGQLSVYLRLTGSKVPSIYGPS
                     ADEA"
     gene            2691..4241
                     /locus_tag="PLT35_04525"
     CDS             2691..4241
                     /locus_tag="PLT35_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28524.1"
                     /translation="MTGGGGDRAGRWPARWLALGALVFAVVATLNSGGYRYGVGDQAF
                     YLPAVQRHLEPASFPHDRPVIDDQDRLSVFPSGAAAVVRATGMSRPALFGLVYLASLF
                     GLFLAARSLAAALGFSTWAQAALLAALTIKHRVGLTGVNTLEGYAHPRELAFALGVGA
                     VSCVLRRKPAAALGLVASAFAVHPTTALWFGVWVGVALLAAEPRLRAWLVPAAAAAAG
                     ASAWAVVWGPLGGQTVRMDGVWLGVLTVKDYLLPTGWPAAGWGVALAYVAAVAGGFAL
                     RRSAGLACPREGALVAGLAALLAVFVASLPLVHARVALAVQFQVSRVFWMFDLVATAY
                     AVWAAVDGRRAASFGPGKLAAPRRAIAAALLLVTAAAARGAWVMWVEHPGRSFVRIGL
                     PHDDWQDAMAWLSRTPLDTHVLADPGHAWRYGTSVRVAAGRDVLLEEVKDSAMALYSR
                     RVAARVAERIGALGDFGSLSPEGARALAAHYDLDYLVTDHRLDLPMVYRNARFTLYRL
                     GPPVAPPR"
     gene            4309..5376
                     /locus_tag="PLT35_04530"
     CDS             4309..5376
                     /locus_tag="PLT35_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG28525.1"
                     /translation="MHPFAPPRWLRGGHRMTLYAWARRRRFPGLPPAEPRYFQTEPHT
                     RVLGYCHWQPDRTAGPVLVVLHGLEGSSDSHYVLGTAHKACRAGFHVVRLNQRNCGGT
                     EHLTPGLYHSALIDDVRAVLEELTAADGIGPVAVCGFSLGGNVALRLAGHYGDRVPPW
                     LHAVCAISPTLDLGACTTMIERPANRLYEWNFLYGLLRRIRRKARLFPGRYDVSGLWR
                     VRTIRAFDERFTAPDAGFASAADYYDRASSIQVADRIRVPTLIISAEDDPFIPTAPLR
                     DARVAGNPFVTVAAERHGGHCGFVERPRGEYDGYWAEATAVEFASSRARPRAGAAGGA
                     SPALSGPRASPGLQWRPADAT"
     gene            5487..7115
                     /locus_tag="PLT35_04535"
     CDS             5487..7115
                     /locus_tag="PLT35_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG28526.1"
                     /translation="MGERVLLGDEAVALAAVHAGLTAAYGYPGTPSTEILEYLIRHRE
                     QHGSPRAQWCANEKTAYEAALGVSFAGRRALVTMKHVGLNVAADPFMNSALVAITGGL
                     VLAVADDPGMHSSQNEQDSRLFADFARVACFEPATHQEAYDMTLEAFDVSERFEIPVM
                     VRLVTRLAHSRGIVRTGDGRGENPSDKRGQAASWILLPANARRRWRALIERQRDIREY
                     TAGSRFNTVEGGPAKRFGVITTGLARNYLLENLADLPERPALLHVGAYPYPAEAIRAF
                     VAPLARVLLLEEGYPYIERALRGIVPPAALEIQGRESGELPPDGELNPDIVRAALGLP
                     LRQGVTLEGLTLPNRPPQLCAGCPHGDSLHAITSALASIPGAVVTADIGCSTLGALPP
                     YNAVETCVCMGASIGMAKGAADAGVRPVVAVIGDSTFLHSGITPLMDAVKDGTPMTLV
                     ILDNKTVAMTGGQPTILSSSELVTLVHGLGVPPGHLHVIEAHPRRASENTETLRRAVG
                     HPGLSVVIAVRECVETVKRGKREKPSAAAGEVRP"
     gene            7112..7693
                     /locus_tag="PLT35_04540"
     CDS             7112..7693
                     /locus_tag="PLT35_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG28527.1"
                     /translation="MKFDIILAGVGGQGVLSLSAIIASSAMREGLAVKQSEVHGMSQR
                     GGAVLAHLRLADGPIASDLIPRGSAAMILGMEPLESLRYLEYLDRAGAVVSATTPMAN
                     IADYPPLDQVLAAIRSLPRAYLVEAEALARQAGSARASNMVMVGAASPLLPVALSTLE
                     HFVETAFAAKGAKVVETNLRALHAGRAAAAVIP"
BASE COUNT          913 a         2798 c         2803 g         1183 t
ORIGIN      
        1 gggctgccgc tcatcgccgc cccgttcttc gccgtcggcg ggtaccccgc cgtcgtgctg
       61 ctgatgatcc ttcttgcggc ggctgcgtcc ggcctcgcgt ggacgtgggc gcggcgcatc
      121 accggctccg tgtccagcgc gaccttcgcc tgggccgcga caacgctttc attgccgttc
      181 ctcgccaacg ccggcaccgt gttccccgag gtgcccgcgg cgctggccgt tctggccgcg
      241 gtgacggcag cgttccggga agaggcgtcc gggaacgcct cgatcgcatc ggccggatgc
      301 tcggccggtc cctggcgcgc gctggccgtc ggcctggctg ccgccgccct gccctggctg
      361 cacgcgaagt acgcgccgct ctcggccgtc ttgatcgcgg ttggcgcgcg gcgcctctgg
      421 cgtgatcggc cggcggacgg acgggcgggg acgcgcctca tggttgtcgc cgccgccccc
      481 tacgccgcca gtctggcggg gtggatggct ttccactacg cagtgtgggg ttccccgtgg
      541 ccgtccgcgc cgtacggcgg ggcgtcgggc acgcagatga gcgcctggac gtggattcgc
      601 ggcgttcccg gcctgctcgc cgaccaggaa tacggcgtgc tcgcgtacgc gcccgccctg
      661 gcgatcgcct tcctgggcgt ccgttcgatg tggcgggcag gcggacgcgc ccggggagtg
      721 gcgacggact tgtccctgct tgtcgccgcc atgctcgcca ccgtcggagc gttccaaatg
      781 tggtggggcg gcagctccct ccccggccgg atgctcgtgt cggtgatccc gctgctggcc
      841 gtgcccgtgg cctgggaatt ccgcgcgtcg gccgagcggc cggagcggcg cgccgcgtac
      901 cggcttctgc tgatcgtcgg cttcgccctg gcgctggccg cggtcctggc gcgcggcgga
      961 gcctcgctgg cgctgacccg caacggcatt tcccggctga tcgagtggct gtcgccggac
     1021 tggcacctct gggcctatgc gccggacttc atcatgcagc ccgcgtgggt cgcgtggctg
     1081 cagcttgcga tctgggccgc ttgcatcctg gcctgtgggt gggccgtggg aacgctcagc
     1141 gcgcgccgga gcgccgcccg tggggcgtcg cgcacggggc ggggccgcgc cttcctgcgg
     1201 gccgacgccg gtgctttctt cgcgctggtg ctcgtgacgc tcctcatgcc gccggcgctc
     1261 ggcagccgcc tgaaggagaa cccggcgccc gaggagcgga cgcggatcgc gctgctcgag
     1321 tccttcgacc cgcacgcgcg cccgattggc gtccgttacg acccgctgtc actcgtggac
     1381 gccgcgtcgc tgcccggggt cttcacgctc tctgcgcgcc ccggatcgcg caccgccagg
     1441 cagcccgcgc ccgtcctgct caacgcccgg ttcgcgctgc cctccgggcg ctacgaagtg
     1501 gagatcgcgt cccgttcgcc ggcggcgctc gccggcagcc tcgcgctaca ggcggggcgc
     1561 tccgggggct ctctccagga atggagcgtc gccgcgggcc ctggcgagcg atggcgggcg
     1621 acgttcgact tgcccgtgga catcaactac gtcgggttcc gcgcctccga cggcctcgcg
     1681 ccgcaggtgg gcgaactgcg catccggccg ctgcgagtcg tgacgaacct cgatcgagtg
     1741 gcggcgtccg aggtcctcgc ctccagtgcc ttcagccact acgtgttcct gttccacgac
     1801 ggggcggcat acccggagcc tgccggcttc tgggtgcgcg gcgccggtcg cgcggtcgtc
     1861 tcggtcgtgt cccgcacggg ccgcctcgcc acgaacagcc ggatgcggct gcggctgctg
     1921 agcctgatcc ccaacgcggt gagcgtcgag acgccggacc ggacgtggac ggtggagttg
     1981 gaggctggcg tggccagcga gatcgagatc cagccgaccg tgctcgacgg cacgctcagg
     2041 atggccctga gaccggcgtc gggattccgt ccgtccgagc gcacgcccga caacccagac
     2101 cgccggttcc ttggatgctg ggtggagctg gccgagctgt agcgccggcg cgcgccgggg
     2161 cggcagcgcg cgaagcaggc gacgaacaag aggtgacgca tggcactggt ggatgcactg
     2221 ctggcggagt acgaccacga actggccgtc acgaggaagg tgctcgagcg cgtggacgag
     2281 gcgcggctcg gctggcagcc gcacgagcgc tcgatgtcgc tcgggcgtct ggcgaaacac
     2341 gtggccgagg ttccgcgctg ggcgcgcacg atcctcgacg acgccgagtt cgacctggcg
     2401 gacgaggcgg acgggccggc ggcctccgag agcctgtccg acctgctcac cgccttcgac
     2461 ggcctcgtgc tcggggtccg tgcgcgcctc gcggcgaaga ccgacgccga gctggcgacg
     2521 gcgtggacgc tgaagcggaa cgggcaggac ctgttcagca tgccccggtc ggcggcgtgg
     2581 cgcaccttga tcatgaacca cctgattcac caccgcggcc agctcagcgt ctacctgcgc
     2641 ctcactgggt ccaaggtgcc aagcatctac ggcccgagcg ccgacgaggc atgacaggcg
     2701 ggggcgggga tcgcgccggg cgatggccgg cgcgatggct ggccctcggc gccctcgtct
     2761 ttgctgtcgt cgccacgctc aacagcggcg gttatcgata cggcgtcgga gaccaggcgt
     2821 tctacctgcc ggccgtccag cgccacctcg agccggcgtc ctttccccac gaccgcccgg
     2881 tcatcgacga ccaggaccgg ctgagcgtct tcccgagcgg agccgccgcc gtcgtgcgcg
     2941 cgacggggat gtcccggcct gcgctgttcg gcctcgtgta cctggcctcg ctgttcgggc
     3001 tcttcctggc ggcccggtca ttggcggccg cgctcggttt ctcgacgtgg gcgcaggccg
     3061 ccctgcttgc cgcgctgacg atcaagcacc gtgtcggcct gacgggcgtc aatacgctgg
     3121 aaggctacgc gcatccgcgc gagctggcat tcgcgctggg cgtcggcgcc gtgtcgtgcg
     3181 tgctccgccg aaagcctgcc gccgcgctgg gcctcgtggc atcggccttc gccgtgcacc
     3241 cgaccacggc gctctggttc ggcgtgtggg tcggtgtggc gctcctcgcc gcggagccga
     3301 ggttgcgcgc gtggctcgtc cccgccgccg ccgcagcggc tggcgcgtcg gcgtgggcgg
     3361 tcgtatgggg tccgctcggc ggccagacgg tccgcatgga cggagtctgg ctcggcgtgc
     3421 tgaccgtcaa ggactatctc ctcccgaccg gctggccggc agccggctgg ggcgtggcgc
     3481 tggcctacgt ggcggcggtg gcgggcggtt tcgcgctccg tcggtccgcc gggctggcgt
     3541 gtccgcgcga gggagcgctc gttgccgggc tcgccgcgct ccttgcggtg ttcgtcgcgt
     3601 cgctgcccct cgtccatgcg cgcgtggcgc tggccgtcca gttccaggtg tcgcgcgtct
     3661 tctggatgtt cgacctcgtg gcgacggcct acgccgtctg ggcggcggtg gacggccgca
     3721 gagcggcctc cttcgggccg ggcaagctgg ccgcgccccg gcgcgccatc gctgcggccc
     3781 tcctcctggt caccgcggca gccgcccgcg gcgcgtgggt gatgtgggtc gagcatcccg
     3841 gccgctcctt cgtccggatc ggcttgccgc acgacgactg gcaggatgcc atggcgtggc
     3901 tctcgcgcac cccgctcgac acgcacgtcc tcgccgaccc gggccacgcg tggcgctacg
     3961 gcaccagcgt gcgcgtcgcc gccggacgcg acgtcctgct cgaagaggtc aaggacagcg
     4021 cgatggcact gtactcgagg cgcgtcgccg cgcgcgtggc ggagcggatt ggcgctctcg
     4081 gcgacttcgg atcgctctcg cccgagggcg cccgtgcgct ggccgcgcac tacgacctcg
     4141 actacctcgt gaccgaccac cggctcgatc tgccgatggt ctaccggaac gcgcggttca
     4201 cgctctaccg actcggcccc ccggtcgcgc cgccgcgctg aggcgcgccg tgcggcggca
     4261 cggcctcgcc ctccctggag gccggcgccg gtgcgataga ctggaaacgt gcacccgttc
     4321 gctccaccgc ggtggctgcg cggcggccac cgcatgaccc tctacgcctg ggccaggcgc
     4381 aggcgcttcc caggcctccc gccggccgaa ccgcgctact tccagaccga accgcacacg
     4441 cgcgtgctcg ggtactgcca ttggcagccg gatcggacgg ctggccccgt gctggtcgtc
     4501 ctgcacggcc tcgagggttc gagcgactcg cactacgtgc tgggaacggc gcacaaggcc
     4561 tgccgggcgg gcttccacgt cgtgcgcctc aaccagcgca actgcggcgg cacggagcac
     4621 ctgacgccgg ggctgtacca ctcggcgctg attgacgacg tgagggcggt cctggaggag
     4681 ctcacggccg ctgacggcat cggccccgtc gccgtctgcg ggttctcgct cgggggcaat
     4741 gtggcgctca ggctcgccgg gcactacggc gaccgcgtgc cgccgtggct ccacgccgtg
     4801 tgcgccattt cccccacgct cgacctcggc gcgtgcacga cgatgattga gcggcccgcc
     4861 aaccggctct acgagtggaa cttcctgtac ggcctgctcc gccgcatccg caggaaggcc
     4921 cgtttgttcc caggccggta cgacgtgagc gggctgtggc gagtgcggac gatccgggcc
     4981 ttcgacgagc gcttcacggc cccggacgcc ggattcgcca gcgcggccga ctactacgat
     5041 cgtgcctcgt cgatccaggt ggcggaccgc atccgcgtcc cgacgctcat catctcggcc
     5101 gaggacgatc cgttcatccc gacggcaccg ctgcgcgacg cgagggtcgc cggcaatccg
     5161 ttcgtcacgg tggcggcaga gcgccacggt ggccactgcg gattcgtcga gcggccccgg
     5221 ggcgagtacg acgggtattg ggccgaagcg acggccgtcg agttcgcgtc ctcccgcgcc
     5281 cgcccccgag cgggtgcggc gggcggggct tcgcccgccc tgtcgggtcc gcgtgcgtcg
     5341 cccgggctac aatggcggcc ggcggatgcg acctgacggc ggcgtccccg aggcgatccg
     5401 gcggcagccg cctctggttc ggggccgatc gcgcggcccc ggcgtgcggc cgggttcccc
     5461 ggtcggcgtg agtcgaggag tctgacatgg gagagagggt cctgctcggc gacgaggcgg
     5521 tggcgctggc ggcagtgcac gcgggcctga cggcggccta cgggtaccca ggcacaccgt
     5581 ccaccgagat tctcgaatac ctcattcggc accgtgagca gcacggcagc ccgcgggccc
     5641 agtggtgcgc gaacgagaag acggcgtacg aagccgccct cggcgtctcc ttcgccggcc
     5701 gccgcgccct cgtgaccatg aagcacgtgg gcttgaacgt ggcggcggac ccgttcatga
     5761 actcggcgct cgtcgccatc actggcggcc tggtgctcgc ggtggccgac gatcccggca
     5821 tgcacagctc gcagaacgag caggacagcc ggttgttcgc cgacttcgcg agagtggcct
     5881 gcttcgagcc cgcgacgcac caggaggcct acgacatgac gctcgaggcc ttcgacgttt
     5941 cggagcggtt cgagatcccg gtcatggttc gcctcgtgac ccgcctggcg cacagccggg
     6001 gcatcgtccg aaccggcgac gggcgcggcg agaacccctc ggacaagcgg gggcaggccg
     6061 cctcctggat cctgctgccg gcaaacgccc ggcgccggtg gcgcgccttg atcgagcgcc
     6121 agcgcgacat ccgggagtac acggccggct cgcggttcaa caccgtggaa ggcggtccgg
     6181 cgaagcggtt cggggtcatc acgaccggcc tggcgcgcaa ctacctgctg gagaacctcg
     6241 ccgaccttcc cgagcggccg gcgctcctgc acgtgggcgc ttacccgtac ccggccgagg
     6301 cgatccgggc gtttgtcgcc ccgctggccc gcgtgctgct cctcgaggag ggctacccgt
     6361 acatcgagcg cgcgctgcgc ggcatcgtgc cgccggcggc gctcgagatc cagggacgcg
     6421 agtccggcga gctgccgccg gatggcgagc tcaacccgga catcgtgagg gccgcactcg
     6481 gcctgccgct gcgccagggc gtcacgctgg agggcttgac gctgcccaac cgcccgccgc
     6541 agctgtgcgc cggctgtcct cacggcgaca gcttgcacgc gatcacctcg gcgctggcgt
     6601 ccatcccggg cgcggtggtg acggccgaca tcggctgctc gacgctgggc gcactgccgc
     6661 cgtacaacgc cgtcgagacc tgcgtgtgca tgggcgcctc gatcggcatg gcgaagggtg
     6721 ctgcggacgc gggggtccgt cccgtcgtcg ccgtcattgg cgacagcacc ttcctccact
     6781 cgggcatcac cccgctcatg gacgcggtca aggacggcac gccgatgacc ctggtcatcc
     6841 tcgacaacaa gaccgtcgcg atgacgggcg ggcagcccac gatcctgtcg tcgtccgagc
     6901 tcgtgacgct cgtgcacggc ctgggcgtgc cgccgggcca cctgcacgtc atcgaggcgc
     6961 atccgcggcg cgcctccgag aacaccgaga cgctgcggcg cgcggtgggc cacccgggcc
     7021 tctcggtcgt gattgccgtc cgggagtgcg tggagaccgt gaagcgcggg aagcgggaga
     7081 agccgtccgc cgcagccggg gaggtgcgcc catgaagttc gacatcatcc tcgcaggcgt
     7141 cggcgggcag ggcgtgctct cgctgtcggc catcatcgcc tcgagcgcga tgcgcgaggg
     7201 gctcgccgtc aagcagtccg aggtgcatgg catgtcgcag cgcggcggcg cggtgctcgc
     7261 ccacctgcgg ctggcggacg ggcctatcgc cagcgatctc atcccgcgcg ggtccgcggc
     7321 gatgatcctc gggatggagc ccctcgaaag cctgcgctat ctcgagtacc tggatcgtgc
     7381 cggcgccgtc gtctccgcga caacgccaat ggcgaacatc gccgactatc cgccgctcga
     7441 ccaggtgctg gccgccatcc ggagcctccc gcgggcgtat ctcgtggagg cggaggccct
     7501 ggccaggcag gcgggctccg cgcgcgccag caacatggtg atggtgggcg cggcgtcccc
     7561 gctgctgccc gtggcgctct ccacgctcga gcacttcgtg gagacggcct tcgcggccaa
     7621 gggggccaag gtcgtggaaa ccaacttgag ggccctgcac gcgggccggg cggccgccgc
     7681 cgtcatcccg tgagcgc
//
LOCUS       DAOSIF010000088         7603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8890_length_7603_cov_3.519210, whole genome shotgun sequence.
ACCESSION   DAOSIF010000088 DAOSIF010000000
VERSION     DAOSIF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8890_length_7603_cov_3.519210"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..465
                     /locus_tag="PLT35_04545"
     CDS             124..465
                     /locus_tag="PLT35_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOG28528.1"
                     /translation="MHEYALMQALMDRVGEEAAARRALAVHRIVVRIGDLSGVEADLL
                     ESAFEIVRAGTMCEGAALEIERVPATWVCSGCGQPVPSGGRLQCGGCGFPARLAAGGE
                     ILLGRIELEVP"
     gene            465..1196
                     /gene="hypB"
                     /locus_tag="PLT35_04550"
     CDS             465..1196
                     /gene="hypB"
                     /locus_tag="PLT35_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOG28529.1"
                     /translation="MCEYCGCGDPHRVSVDLRGEILAANDRQAAHNREHFAAHGVLAI
                     NLMGSPGSGKTAVLEATAAALGSRARIAAITGDLETERDAERLRAAGIPARAITTGSA
                     CHLDAGMIHEALHDEALHGASLLVIENVGNLVCPAVYDLGQAFNVVVLAVTEGEDKPL
                     KYPVMFRKADLVLVTKTDLLPHLGLSLELLDDAIARVMPTPAVFRVSARTGEGMDRWF
                     AWLDAHLASRPGPDRAHAAFGHRHG"
     gene            complement(1257..2489)
                     /locus_tag="PLT35_04555"
     CDS             complement(1257..2489)
                     /locus_tag="PLT35_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28530.1"
                     /translation="MSAMPPEGRFATPVPEQPGGSLPCPRCDRLVAPSLVSCPHCGVD
                     LALAAVLAERQALSVLPVEPDAPFVGDAMLSRFGEFLMKKGYITEAQLEGALARQGEI
                     ARMGMRETLGQVLLEQRVMTREQLELASVEQVQELQNALRAANAQLEQRVAERTRELE
                     RAYRRLTELDRLKSNFISNVSHELRTPLTKIKGFHALLSAGELGPLTGEQEQAVEIMG
                     RGVAELERLVGDLIQFATGAKGEMALSLAPFPAGPLVEGAVAAARARAETRGIALACR
                     PIAAGLTVTGDVERLRWVMAQLLDNAIKFTQPGGSVEAGAKPVEGGVRFWVADTGTGI
                     SEERLPELFEPFHQLDGSATRKAGGTGLGLALVKMIVSAHGSTVSVVSRPGVGSRFSF
                     DLGTSAEAAPDATADAGG"
     gene            complement(2545..3918)
                     /locus_tag="PLT35_04560"
     CDS             complement(2545..3918)
                     /locus_tag="PLT35_04560"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOG28531.1"
                     /translation="MPYSTIIEPFRIKSVEAVKFTTREERREALVRAGYNVFRLHAED
                     VLIDLLTDSGTGAMSAAQWSAIMQADESYAGSRSFYRFESVVRDLTGFAHVIPTHQGR
                     AAEHILFRTRLRPGQVVPNNNHFDTTRANVEAEDVEALDLVIEEGRVPGLIHPFKGNM
                     DLGALERLLETRGERVPLVMVTVTNNSGGGQPVSLENLRGVRRICDRYRKPFFLDACR
                     FAENAWFIKTREPGQAGRSPKAIAQEMFSLADGCTMSAKKDGLSNIGGFLAMNDPGWA
                     EAARNLLILTEGFPTYGGLAGYDLEAIARGLEEVVEEEYLRYRIRSIEYLGEKLIAAD
                     VPIVRPPGGHAIYLDARALLPHVPPLQYPGVALANQLYLEAGIRGAEIGTVMFGLRPD
                     GSERPGPMDLVRLAIPRRVYTQSHVDYVAEAVIAVAGLRGRMRGYRIVSAPKALRHFT
                     AAFAPLE"
     gene            4071..4268
                     /locus_tag="PLT35_04565"
     CDS             4071..4268
                     /locus_tag="PLT35_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG28532.1"
                     /translation="MPLFEFRCKSCGQHFETLVIGSRTTVCPRCDSADLEKQASRFGV
                     GGPGGDRSAGHSRPSCSTGGG"
     gene            4405..>7603
                     /locus_tag="PLT35_04570"
     CDS             4405..>7603
                     /locus_tag="PLT35_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOG28533.1"
                     /translation="MKRLALLLACAAAAAIATPTITLHSQAPVAGESRLLRFPAIHGN
                     RIVFTYAGDLYTVPAAGGTARRLTSDIGFEMFARFSPDGERLAFTGQYDGNTEVYVMP
                     SDGGVPKRVTYTATLGRDDVSDRMGPNNIVIGWKNATQILFRSRRTEWNDFRGQLYLA
                     SVDGGPIEEVPLPRGGFGMFSPDGRQFVYNRVFREFRTWKRYRGGQADEVWLHDFAAK
                     TTTNLTNNPAQDIIPMWRGSRVYFASDRGDRGRMNLYSYDLATRQTKALTSFAEFDVK
                     FPSLGDAAIVFENGGWIYRLDLATERAARVPIVIREDFDSGRGAIVDVSRSITTSDIA
                     PDGSRALFGARGDVFTVPAKHGPTRNLTRTPGVHERDARWSPDGRWVAYISDATGEDE
                     IYVAPQDGRGAAAQVTSGNANYMYQPLWSPDSKKLLWANRQQQLHVVDVATKAVTRVA
                     ESEAFEIRDYAWSPDSRFVAYAKPEVQGPQRIYLYSLDSRRTIAATDAWFASSSPAFS
                     PDGKLLFFVSARSFSPIYGQTEFEHIYADMSRIYFVTLAKDTKSPFAPKSDEVKVSDE
                     GAPADAKTGAAKAPGASAPAASAAPPGRDGGAPALKVDEDGLMERIGVVPTPPGAYRS
                     LAPAGNRIYYVRTNAREAPALYVYDLEKQAETQIGAGLGFALSADGKKMLLAQQGQRY
                     AIIDTPTGKAEPREFLDLGDLKVTLDRHAEWRQIFHESWRQMRDFFYAPNMHGVDWPG
                     ARRTYEPLLAHVNHRADLTYVIGEMIGELNAGHAYVGGGDLPRLERVPMGLLGARLER
                     DPQSKAFRITRILKGQNWDPALRSPLTDIGVDARVGDYILAVDGRPTAALSDIWEALV
                     GKAGKQVTLSLNARPSMEGARETVVVPVEDERGLYYFNWVQGNLEKVAKATGGRVGYM
                     HIPDMGVPGLNEFAKYFYPQTEKEAMIVDVRSNGGGNVSPMIIERLRRELVMVNIARN
                     GNPQANPGAMILGPKVLLADEFSASDGDLVTYRFKTHRIGPVIGKRTWGGVVGIRGTL
                     PLLDGGVLNRPEFSRYDVAGKSWVIEGVGVEPDIEV"
BASE COUNT         1163 a         2680 c         2663 g         1097 t
ORIGIN      
        1 cgcgtgcatc ggccggtgca tcggcgccca cgcgggcgcg gtcatcctcg ataccggagt
       61 gggcaggcgc ctactcgccg agccggaagg cgagccgctt ccaaggatct gctgaggccg
      121 gcgatgcacg agtacgccct gatgcaggcc ctcatggatc gcgtcggcga agaggctgca
      181 gcccgccgcg cgctcgccgt gcaccgcatc gtggtgcgta tcggcgatct ctcgggggtg
      241 gaggcggacc tgctcgagag cgccttcgag atcgtccggg ccggcacgat gtgcgagggc
      301 gccgcgctcg agatcgagcg cgtgcccgcg acatgggtgt gctcggggtg cgggcagccc
      361 gtccccagcg gcgggcggct gcagtgcggg gggtgcgggt tccccgcccg gctcgcggcg
      421 ggcggcgaaa tcctgctcgg ccgcatcgaa ctggaggtgc cctgatgtgc gaatactgcg
      481 gatgcggaga cccgcaccgg gtctcggtcg acctgcgcgg cgagatcctc gcggcgaacg
      541 accgacaggc ggcccacaac cgcgagcact tcgcggcgca cggggtgctc gcgatcaacc
      601 tgatgggatc gccgggatcc ggcaagaccg cggtgctcga ggcgacggcg gcggccctcg
      661 gctcgcgggc gcgcatcgcg gccatcaccg gcgacctcga gacggagcgc gacgccgagc
      721 ggctgcgggc ggcgggcatc ccggcgcggg cgatcacgac gggctccgcc tgccacctgg
      781 acgcgggcat gatccacgag gcgctccacg acgaggcgct gcacggcgcc agtctgctcg
      841 tcatcgagaa cgtgggcaac ctcgtgtgcc ccgccgtcta cgacctcggt caggccttca
      901 acgtcgtcgt actggcggtg accgagggcg aggacaagcc cctgaagtac ccggtcatgt
      961 tccggaaggc cgacctcgtc ctcgtcacca agaccgacct cctgccccat ctcggcctca
     1021 gcctcgagct cctcgacgac gcgatcgcca gggtgatgcc gacgcccgcc gtcttccgcg
     1081 tgtcggccag gaccggcgaa ggcatggatc gctggttcgc gtggctcgac gcgcacctcg
     1141 cctcgcgtcc cggacctgac cgcgcgcacg ccgcgttcgg gcatcggcac ggctgacgcg
     1201 gcggcgccag ggccgtcggc ggcgcgcgcc gagcagcgtc gggatgcgaa cgggcgtcag
     1261 ccgccggcgt ccgcggtcgc gtcgggcgcc gcctccgcgg aggtgcccag gtcgaaactg
     1321 aagcggctgc cgacgccagg ccggctcacg accgagaccg tcgagccgtg cgccgagacg
     1381 atcatcttga cgagggcgag acccaggccg gtcccgccgg ccttgcgcgt cgccgagccg
     1441 tcgagctggt ggaacggttc gaagagctcg ggaaggcgct cctcgctgat gcccgttccc
     1501 gtgtcggcca cccagaaccg tacgccgcct tcgaccggct tcgcaccggc ctcgacgctg
     1561 ccgcccggct gcgtgaactt gatggcgttg tcgagcagct gggccatgac ccaccggagg
     1621 cgctcgacgt ccccggtgac cgtcagcccg gcggcgatcg gccggcacgc cagggcgatc
     1681 ccccgcgtct cggcccgcgc ccgcgcggct gcgacggcgc cctccacgag gggcccggcc
     1741 gggaacggcg ccaggctcag ggccatttcg cccttggcgc cggtggcgaa ttgaatcagg
     1801 tcgccgacca gccgctcgag ctcagcgacg ccccgcccca tgatctcgac ggcctgctcc
     1861 tgctcgcccg tgaggggccc gagctcgccg gcggacagca gcgcgtggaa ccccttgatt
     1921 ttcgtcagcg gggtccgcag ctcgtgcgag acgttcgaga tgaagttgct cttcagccga
     1981 tcgagctccg tgaggcggcg gtaggcccgc tcgagttcgc gcgttcgttc ggcgacgcgc
     2041 tgctcgagct gggcgtttgc cgcgcgcagc gcgttctgga gctcctgcac ctgctcgacg
     2101 ctggcgagct cgagctgctc ccgcgtcatc acgcgctgct cgaggagcac ctgtccgagc
     2161 gtctcccgca tgcccatgcg ggcgatctcg ccctgccgcg cgagcgcgcc ctcgagctgg
     2221 gcttcggtga tgtagccctt cttcatcagg aactcgccga agcgcgagag catcgcgtcg
     2281 cccacgaacg gggcgtccgg ctccacaggc agcacgctca gggcctgccg ctcggcaaga
     2341 accgccgcga gggcgaggtc gacgccgcag tgcggacacg agacgaggct gggcgccacc
     2401 aggcggtcgc atcgcgggca gggcaggctc ccgcccggct gctccggaac cggcgtggcg
     2461 aacctcccct ctggcggcat ggcgctcatg cggctactcg ggactatcgg cccgcgggaa
     2521 ggcgctcaat agtcgccggc cggcctactc cagcggcgcg aaggcggcag tgaagtgccg
     2581 gagggccttc ggcgccgaga caatccggta gccgcgcatc cggccccgca agccggcgac
     2641 cgcgatgacg gcctcggcga cgtagtccac gtgagactgg gtgtagaccc ggcgcgggat
     2701 ggcgaggcgc acgaggtcca tcgggccggg ccgctcgctg ccatcgggcc tcaggccgaa
     2761 catcacggtg ccgatctcgg cgccccggat ccccgcctcg agatagagct ggttcgccag
     2821 ggcgaccccc gggtactgca acggcggcac gtgcgggagg agcgcgcgcg cgtcgaggta
     2881 gatggcgtgc ccgcccggcg ggcggacaat ggggacgtcc gccgcgatga gcttctcgcc
     2941 gagatactcg atcgaacgta tccggtagcg gaggtactcc tcctccacca cctcctcgag
     3001 gccgcgggcg atcgcctcga gatcgtatcc cgccagcccg ccgtaggtcg gaaacccctc
     3061 ggtcagaatg aggaggttcc gggcggcctc ggcccatccg ggatcgttca tggccaggaa
     3121 gccgccgata ttcgacaggc cgtctttctt ggccgacatc gtgcagccgt ccgccagcga
     3181 gaacatctcc tgcgcgatag ccttcggcga gcgccccgcc tgcccgggct cccgcgtctt
     3241 gatgaaccag gcgttctcgg cgaacctgca ggcgtcgagg aagaacggtt tccggtacct
     3301 gtcgcagatg cggcgcactc cgcgcaggtt ctcgagggac accggctggc cgcccccgga
     3361 gttgttcgtg acggtgacca tcacgagcgg cactcgctcc ccgcgcgtct cgagcagccg
     3421 ctcgagcgcg ccgagatcca tgttcccctt gaagggatgg atgagtcccg gcacgcgccc
     3481 ttcctcgatg acgaggtcca gcgcctcgac gtcttcggcc tcgacgttgg cccgggtggt
     3541 gtcgaagtgg ttgttgttgg gcacgacctg cccggggcgc aaccgggtcc ggaacaggat
     3601 gtgctccgcc gcccggccct ggtgggtcgg aatgacgtgc gcgaacccgg tcaggtccct
     3661 gacgacggat tcgaatcggt agaacgaacg gctgccggcg taggactcgt cggcctgcat
     3721 gatggcgctc cactgcgccg ccgacatcgc tccggtgccg gagtcggtca gcaggtcgat
     3781 gagcacgtcc tcggcgtgca agcggaacac gttgtagccg gcccggacga gggcctcccg
     3841 gcgctcttcc ctggtggtga acttcaccgc ctccaccgac ttgatccgga acggctcgat
     3901 gatcgtgctg tagggcatcc tcgccctccc tggggcgctg gcactttagc acaggccgtc
     3961 cggcgccgaa ggacccggca tgcctccctc cggcaggcgc cgcgacacct ggccgggcag
     4021 cgggtgtcac cttcgcgcct tgcgctgcat ccttgtgaac ggagcggacc atgcctctgt
     4081 tcgaatttcg ctgcaagtcc tgcggtcagc acttcgagac gctggtgatc gggagccgga
     4141 cgaccgtctg ccctcgctgc gacagcgcgg acctggagaa gcaggcctcg aggttcgggg
     4201 tcggcggccc cggcggggat cgctcggccg ggcactcccg cccgtcctgc tcgaccggcg
     4261 gcggttgagg gttctaatcc caccgggccg tgagcccggt ccatcgaccg actgacctcc
     4321 gcggcgcgcg cggcccctgg ccgccgggcg tggcggccga tcccgctggg cgtgtagaat
     4381 ccttcgaact ggagtcacac gcccatgaaa cgcctggccc tgttgctcgc gtgcgcggcc
     4441 gccgccgcaa tcgccacgcc aaccatcacg ctgcactcgc aggcccccgt ggcgggcgag
     4501 tcacggctcc tccgcttccc ggccatccac ggcaatcgga tcgtgttcac gtacgcgggg
     4561 gatctctaca cggttcccgc cgcaggcggc acggcccgcc ggctgaccag cgacatcggc
     4621 ttcgagatgt tcgcgcggtt ctcgccggac ggcgagcggc tggccttcac gggccagtac
     4681 gacggcaaca ccgaggtcta cgtgatgccc tccgacggcg gcgtgccgaa acgcgtcacg
     4741 tacaccgcca cgctgggccg cgacgacgtg tcggaccgca tggggccgaa caacatcgtg
     4801 atcgggtgga agaacgcgac ccagatcctg ttccggtccc gccggacgga gtggaacgac
     4861 ttccgcggcc agctctacct cgcctcggtc gacgggggcc ccatcgagga agtgcccctt
     4921 ccccgcggcg gcttcggcat gttctcgccc gacggccgcc agttcgtcta caaccgcgtg
     4981 ttccgggaat tcagaacctg gaagcgttat cgcggcggac aggccgacga ggtctggctg
     5041 cacgacttcg ccgcgaagac gaccacgaac ctgacgaaca acccggccca ggacatcatc
     5101 ccgatgtgga ggggatcgag ggtctacttc gcgtccgacc ggggcgaccg cggacggatg
     5161 aacctctaca gctacgacct cgccaccagg cagacgaagg cgctgacgtc gttcgccgag
     5221 ttcgacgtca agttcccttc gctcggtgac gcggccatcg tcttcgagaa cggcggctgg
     5281 atctaccgcc tcgacctggc gaccgagcgc gcggccagag tccccatcgt catccgggag
     5341 gacttcgaca gcggtcgcgg cgcgatcgtg gacgtcagcc ggagcatcac gacctcggac
     5401 atcgccccgg acggaagccg ggcgctcttc ggggcgcggg gagacgtgtt cacggtgccc
     5461 gcgaaacacg ggcccacccg caacctcacg cggacgccgg gcgtccacga gcgtgatgcc
     5521 agatggtcgc cggacgggcg gtgggtcgcc tacatctcgg acgccacggg cgaggacgag
     5581 atttacgtgg cgccgcagga cgggcgcggc gcggcggcgc aggtcacgtc cgggaacgcc
     5641 aactacatgt accagcccct gtggtcgccc gacagcaaga agctgctgtg ggccaatcgg
     5701 cagcagcagc tgcacgtcgt ggacgttgcc accaaggcgg tgactcgcgt ggcggagtcg
     5761 gaggcgttcg aaatccggga ctacgcgtgg tccccggaca gccggttcgt ggcttacgcg
     5821 aagcccgagg tgcagggccc gcagcggatc tacctctact ccctcgacag caggcggacc
     5881 atcgccgcca ccgacgcctg gttcgcctct tccagcccgg cgttcagccc cgacggcaag
     5941 ctgctcttct tcgtgtcggc ccgcagcttc agcccgatct atggccagac cgagttcgag
     6001 cacatctacg ccgacatgtc ccgcatctac ttcgtcacgc tcgcgaagga cacgaagagc
     6061 cccttcgcgc cgaagagcga cgaggtgaag gtgagcgacg agggcgcgcc cgcggatgcg
     6121 aagaccgggg cggcgaaggc ccccggcgcg agcgccccgg cggcgagcgc ggcgccgccc
     6181 ggcagagacg gcggcgcccc ggccctgaag gtggacgagg acgggctgat ggagcggatc
     6241 ggcgtcgtcc cgacgccgcc gggcgcctac cgatcgctgg ccccggcggg caaccggatc
     6301 tactacgtgc gcacgaacgc acgcgaggcg cccgcgctgt acgtctacga cctggagaag
     6361 caggcggaga cgcagatcgg agcggggctc ggctttgcgc tctcggccga cggcaagaag
     6421 atgctgctcg cccagcaggg ccagcgctac gcgatcatcg acacgccgac cggcaaggcc
     6481 gagccgcgcg agttcctcga tctgggcgac ctgaaggtca ccctcgatcg ccacgccgag
     6541 tggcggcaga tcttccacga gtcgtggcgg cagatgcgcg acttcttcta tgcgccgaac
     6601 atgcatggcg tggactggcc cggcgcccgg cgcacgtacg aacccctgct ggcgcacgtc
     6661 aaccaccgcg ccgatctcac gtacgtcatc ggcgagatga tcggcgagct gaacgcggga
     6721 cacgcgtacg tgggcggggg cgacctgccc cgcctcgagc gcgtgccgat ggggctgctg
     6781 ggcgcgcgac tggagcggga cccgcagtcg aaggcgttcc gcatcacccg gatcctgaag
     6841 gggcagaact gggaccccgc gctccgctcc ccgctcaccg acatcggcgt ggacgcgaga
     6901 gtcggcgact acatcctggc ggtcgacggc cggcccacgg ccgccctctc cgacatctgg
     6961 gaagccctcg tcgggaaagc cggcaagcag gtaacgctct cgctcaacgc gaggccctcg
     7021 atggagggcg cgcgcgagac ggtcgtggtt cccgtcgaag acgaacgcgg cctctactac
     7081 ttcaactggg tccagggcaa cctggagaag gtcgcgaagg ccaccggcgg ccgggttggc
     7141 tacatgcaca ttccggacat gggcgtcccg ggcctgaacg agttcgccaa gtacttctat
     7201 ccgcagaccg agaaagaggc gatgatcgtc gacgtccgaa gcaacggcgg cggcaacgtg
     7261 tcgccgatga tcatcgagcg gctgcggcgc gagctggtca tggtgaacat cgcgcggaac
     7321 ggcaacccgc aggcgaaccc gggcgcgatg atcctcggcc cgaaggtgct gctggccgat
     7381 gagttctcgg cctccgacgg cgatttggtg acctaccgct tcaagacgca caggattggc
     7441 cccgtcatcg gcaagcggac ctggggcggc gtcgtcggca tccgcgggac gctgcccctc
     7501 ctggacgggg gcgtgctgaa ccgtccggag ttctcgcgct acgacgtcgc cgggaagagc
     7561 tgggtcatcg agggcgtggg cgtcgagccg gacatcgaag tgg
//
LOCUS       DAOSIF010000089         7600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8897_length_7600_cov_4.272101, whole genome shotgun sequence.
ACCESSION   DAOSIF010000089 DAOSIF010000000
VERSION     DAOSIF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_7600_cov_4.272101"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLT35_04575"
     CDS             <1..417
                     /locus_tag="PLT35_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28534.1"
                     /translation="VTVFPEGRLSWGQRLRARSGLGLLAGWAPEAKVVLCAITGSTDF
                     VRFPTRPRFTVSFFPPATGGIRPGEEPAAFSARMLQELRARAPVTPAGRKRIIGGSPK
                     VRRTLARNRTLNELPPEPPPPLSSAPPPQPGEQMGD"
     gene            complement(687..3941)
                     /locus_tag="PLT35_04580"
     CDS             complement(687..3941)
                     /locus_tag="PLT35_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG28535.1"
                     /translation="MNTRLFALLCLLACGAAAPSALPPAAAPSAPGPGWLRYPAISPD
                     GRTIVFTYKGDLYRVAAGGGEAAPLTAHPAHDFMPVWSRDGRQIAFASDRYGNFDVFV
                     MPAAGGEARRLTFHSAPEYPYAFTADGAAVVFGAARMDAAENRQFPAASQPELYQVAA
                     AGGRPIQLLTTPAEDAKFSRDGRLLLYHDKKGGENAWRKHHTSAIARDIWVCDTRTGL
                     HRRLTTFAGEDRSPVFAAGERAFYYLSEESGSFNVHRMSLDGGATERLTSFTQAPVRF
                     LSASDTGVLCFGYDGGVYTMTPGSAPRKVEIAIAADAKANRERVIPVTGGAREMAVAP
                     SGKEIAFIARGDVFVTSVEGGVTKRITATPEDERGVGFSPDGKALVYASERNGRWAIY
                     EARRARDAEPHFYASTLVQETPLVANAQQNTQPRHSPDGREIAFLENRNTLKVLNLES
                     KQARTILTDKEIFSSGSLHYRWSPDSKWILFDYAIPGIAPGEVGLVRADGKSAAANLT
                     QSGFDDGAAEWILGGKAMLWLSNRDGLKSVAQSGAAQRDAYAMFFTKDAWDRFRLTKE
                     EYALVKEAEEQKDKDKPKAGADQEKKDAAKDAKVEEVVVDFDGLEIRKARLTIHSSAL
                     SDALVSRDGDTLYYLARFEKHANLWSTNLRTQETKMLVALNSSGGSLAWDKDQKTIFL
                     LADGSLSKIDPAGGTRQSIGISGEVVADLDAEREAMFDHVWRRTRDTFYTAGFHGVDW
                     QAIRPMYAKHLPHIGNNHEFAELLSEMLGELNVSHSGSTFSPSSPTDDATGALGVFYD
                     QAYTGPGMKVVEVVKDGPLDKAGMSVTPGSIIEAVDGQAITPATDIAQCLNRKAGKPV
                     LLVVATGDKKAEIVAKPVTLAEENRLLYARWVRRNQAEVDRLSSGQLGYIHVPGMNDG
                     AYRTTFEEALGKYALRKGLVVDTRFNGGGDLVADLAMFLSGKRFFEYTTDTRSNGFEP
                     NFRWTKPSVSLAGEANYSDGHCYAYAYKALKIGPLVGMPVPGTCTFAGWDMLQDGLRW
                     GVPGVGVKDSTTGKYLENLQTEPDLRLMNDYAVVGKGRDQQLEAAVAELLKLVR"
     gene            complement(4023..4538)
                     /locus_tag="PLT35_04585"
     CDS             complement(4023..4538)
                     /locus_tag="PLT35_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28536.1"
                     /translation="MLPRADLPGEGLTRVDFEQLRSLGAIALYATTFVVALVSGLVPY
                     VLSFEVFVLAVATLTNAPIAPIVGLGVLGQAITKYILYLAGTGTLNISWVRRTATSRA
                     AEAFARHPHASLGVVLSSAAFAVPPLYPTSVIAGTLRLPQIAFVLVVAAGSIVRYSAL
                     FLAPKLIRPLF"
     gene            complement(4643..5329)
                     /locus_tag="PLT35_04590"
     CDS             complement(4643..5329)
                     /locus_tag="PLT35_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG28537.1"
                     /translation="MIQRPIALACLWLLLPEAPMTVDHIHWLGHASFRIEDGAAQIYI
                     DPWKLPALAPKAAVVLVTHGHSDHFSPGDIARIEQPGTVFVAPADVAAKLKGRRVVIA
                     ASGGTFAAGPIRVEAVPAYNVGKPFHPRASGWLGYIITLSDGSRVYHSGDTDAIPEML
                     ALANIDVAMMPCGGTYTMTAAEMAAAANGFKPKVLIPMHWGDLVGSKADAEAVKKAFA
                     GTTVIKPVER"
     gene            5744..>7600
                     /locus_tag="PLT35_04595"
     CDS             5744..>7600
                     /locus_tag="PLT35_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOG28538.1"
                     /translation="MGAEKPRAAFVPHADRGGALARDRTRSPYFRLLNGTWKFRWAKN
                     PFSLPNGFESPAYDDADWDPMPVPSNWQVVGANEGRPYDRPFFTNIKHPFRADPPRVP
                     HDDNPVGLYRTAFEVPAGWRGRSVFVHFAGVQSAYYVWLNGTRIGYREDAFTPGEFDL
                     TPHLRPGRNVLAVEVLHHSDGSYLEDQDYWRLAGIFRDVYLVAQPAVRLRDFLVRTDL
                     DAAYRDAVLEVRASLENRSGAAARGHALRAALVGPDGREVFAADLAGPAQIAPDAEAE
                     LRASGAVRAPALWTAETAALYTLVVEHLDASGAVLEAASARVGFRKVEIRGGQLLLNG
                     VPITFRGVNRHEFDPDRGRVVSRERMLEDIRLMKQFNFNAVRTSHYPNDPLWYDLCDE
                     LGLYVVDEANVESHELWERKVYIGDDPAWTAAFVARGIAMVERDKNHPSVVYWSMGNE
                     TGLGRNFDAMYAAMKAVDPTRPIHYESRNPPYAPTLSSYDIISTMYPAVDHIIDLMNQ
                     DPSRPVIICEYAHAMGNSLGNFKKYWDAYDAYPRLQGGFIWDWVDQALRHPGPDGRVR
                     WNWVNTSDGSNANDGLVNADRTPQPELFEAKKVQQPVKVEGVDLARGRVRVTN"
BASE COUNT         1054 a         2691 c         2684 g         1171 t
ORIGIN      
        1 gtcacggtgt ttcccgaggg ccggctgtcc tgggggcagc gcctgcgcgc gcgcagcggc
       61 ctggggctgc tcgccggatg ggcacccgag gcgaaggtag tgctctgcgc gatcaccggt
      121 tccaccgatt tcgtccggtt ccccacgcgg ccgcgcttca ccgtgtcgtt cttcccaccg
      181 gccaccggcg gcatccgtcc gggcgaagag ccggcggcgt tttccgcccg catgctgcag
      241 gaactgcgcg cccgcgcgcc cgtcaccccg gcggggcgca agcgcatcat cggcggctcg
      301 ccgaaagtgc gccggacgct cgcgcgcaac aggacgctca acgagctgcc gcccgaaccg
      361 ccgccgcccc tttcatccgc gccgcctcct cagccaggcg agcagatggg cgattgagcc
      421 caggcgcagt cttctccccg ttccgccaca acgatcacac cgcgaccccg caagcacata
      481 cgaccgcgcc aaggtcggcc aggcgcccgc cacgatcgac cctggcgatc gcgggtccct
      541 gtcgcggcag gccgaagcct ctcccgacgc ggcgcaggcc cgcctgctgc gggcgaaggc
      601 ggcgatcgtc gaagccgccg ccgcgctcat cacccctgcg ctcgccacga cgaagccggc
      661 gccccggcct cccggcgcgc cacccgctac ctgacgagtt tcagcagctc cgcgacggcc
      721 gcctcgagct gctggtccct gcccttgccg acgacggcat agtcgttcat caggcggagg
      781 tcgggctcgg tctgcaggtt ctccaggtac ttgcccgtcg tcgagtcctt cacgccgacc
      841 cccggcacgc cccagcgcag gccgtcctgc agcatgtccc agcccgcgaa ggtgcaggtc
      901 cccgggacgg gcatccccac gagcgggccg atcttcagcg ccttgtaggc gtaggcgtag
      961 cagtggccgt cgctgtagtt cgcctcgccg gccagcgaga cgctcggctt cgtccagcgg
     1021 aagttgggct cgaacccgtt gctgcgcgtg tcggtcgtgt actcgaagaa ccgcttgccg
     1081 ctgaggaaca tcgccaggtc cgcgaccagg tcgccgccgc cgttgaaccg ggtgtccacg
     1141 acgagcccct tgcggagcgc gtacttgccc agcgcctctt cgaacgtcgt gcggtaggcg
     1201 ccgtcgttca tgcccggcac gtggatgtag cccagctgcc cgctgctcag ccggtccacc
     1261 tcggcctggt tccggcgcac ccagcgcgcg tagagcagcc ggttctcctc ggcgagcgtg
     1321 acgggcttcg ccacgatctc ggctttcttg tccccggtgg cgacgacgag cagcacgggc
     1381 ttgccggcct tgcgattgag gcactgcgcg atgtcggtgg cgggcgtgat ggcctggccg
     1441 tccaccgcct cgatgatcga gcccggggtg acgctcatcc cggccttgtc gagggggccg
     1501 tccttgacga cctccaccac cttcatgccg gggcccgtgt acgcctggtc gtagaacacg
     1561 cccagcgcgc cggtggcatc gtcggtcggc gacgaggggg agaaggtcga gccgctgtgg
     1621 ctgacgttca gctcgcccag catctcgctc agcagctcgg cgaactcgtg gttgttcccg
     1681 atgtgcggca ggtgcttcgc gtacatgggg cgaatggcct gccagtccac gccgtggaag
     1741 ccggccgtgt agaaggtgtc gcgcgtccgg cgccacacgt ggtcgaacat cgcctcgcgc
     1801 tcggcgtcca ggtcggcgac gacttcgccg ctgatgccga tgctctggcg cgtgcctccc
     1861 gccggatcga tcttcgacag gctgccgtcg gccaggagga agatggtctt ctggtccttg
     1921 tcccaggcca ggctgccgcc gctgctgttc agcgcgacga gcatcttcgt ctcctgcgtt
     1981 cgaaggttcg tggaccagag gttcgcgtgc ttctcgaagc gcgccaggta gtacagcgtg
     2041 tcgccgtccc ggctgaccag ggcgtccgag agcgccgagg aatggatggt cagccgcgct
     2101 ttccggatct ccaggccgtc gaagtcgacg acgacctcct cgaccttcgc gtccttcgcg
     2161 gcgtccttct tctcctggtc cgccccggcc ttgggcttgt ccttgtcctt ctgctcctcg
     2221 gcctccttca ccagggcgta ttcctctttg gtgaggcgga accggtccca ggcgtccttc
     2281 gtgaagaaca tcgcgtacgc gtcgcgctgc gcggcgcccg actgggcgac cgacttcagg
     2341 ccgtcccgat tgctcagcca gagcatggcc ttgccgccca ggatccactc ggcggctcca
     2401 tcgtcgaacc cgctctgcgt gaggttggcc gcggcgctct tgccgtcggc ccggacgagc
     2461 ccgacctcgc ccggggcgat gccggggatg gcgtagtcga agaggatcca cttgctgtcc
     2521 gggctccacc ggtagtggag cgagccgctc gagaaaatct ctttgtcggt caggatcgtc
     2581 cgcgcctgct tggactcgag gttcagcacc ttcagggtgt tgcggttctc gaggaacgcg
     2641 atctccctgc cgtcgggcga atgccgcggc tgggtgttct gctgcgcgtt ggcgacgagc
     2701 ggcgtttcct ggacgagggt ggacgcgtag aaatgcggct cggcgtcgcg cgcgcggcgc
     2761 gcctcgtaga tcgcccagcg cccgttccgc tcggaggcgt agacgagggc cttcccgtcg
     2821 ggcgagaagc ccacgccgcg ctcgtcttcc ggcgtggccg tgatccgctt cgtcacgcct
     2881 ccctcgacgc tcgtgacgaa cacgtcgccg cgcgcgatga acgcgatttc cttgccgctc
     2941 ggcgcgacgg ccatctcgcg cgccccgccc gtcacgggga tcacgcgctc gcggttcgcc
     3001 ttcgcgtcgg cggcaatcgc gatctcgacc ttgcgcgggg cgctgcccgg cgtcatggtg
     3061 tacacgccgc cgtcgtaccc gaagcacagc acgccggtgt ccgacgcgct caggaaccgc
     3121 accggcgcct gcgtgaacga ggtcagccgc tccgtcgcgc cgccgtccag gctcatcctg
     3181 tggacgttga acgagccgct ctcctcgctg aggtagtaga acgcccgctc gccggcggcg
     3241 aagaccgggc tgcggtcctc gccggcgaac gtcgtgagcc tgcggtgcaa gccggtccgg
     3301 gtgtcgcaga cccagatgtc gcgcgcgatg gccgacgtgt ggtgcttgcg ccaggcgttc
     3361 tccccgccct tcttgtcgtg gtacagcagg agccgcccgt cgcggctgaa cttcgcgtcc
     3421 tcggccggcg tggtcagcag ctggatgggc cgcccgccgg ccgccgcgac ctggtacagc
     3481 tcgggctgcg acgccgcagg gaactggcgg ttctccgccg cgtccatcct ggccgccccg
     3541 aagacgacgg ccgcgccgtc ggccgtgaag gcgtaggggt actcgggcgc cgagtggaag
     3601 gtcaggcgcc gggcctcccc gccggcggcg ggcatgacga agacgtcgaa gttcccgtag
     3661 cgatcgctcg cgaaggcgat ctgccggccg tcgcggctcc acacgggcat gaagtcgtgg
     3721 gccgggtgcg cggtgagcgg cgcggcctcg cctccgcccg cggccacgcg gtagagatcg
     3781 cctttgtagg tgaagacgat cgtcctgccg tccggcgaga tcgcgggata gcgcagccag
     3841 ccgggcccgg gcgccgacgg agcggcagcc gggggcaggg cggacggtgc ggcggcgccg
     3901 cacgcgagga ggcagaggag cgcgaacagt ctcgtgttca tgggtctcgg actcgaagaa
     3961 ggccggcagt cggcaatcca cggcatggca gcgccgcctg gcgcgggcgg ggcgccttca
     4021 tctcaaaaga ggggccgaat cagtttcggg gccaggaaga gcgccgaata ccggacgatg
     4081 ctcccggccg ccacgacgag gacgaacgcg atctggggca gcctcagcgt gccggcgatg
     4141 acgctggtgg gatagagcgg cggcaccgcg aaggcggcgc tgctcaagac gacgccgagg
     4201 ctggcgtgcg ggtgcctcgc aaacgcctcg gcggccctcg acgtcgcggt ccgcctgacc
     4261 caggagatgt tcagcgtgcc ggtcccggcg aggtagagga tgtacttcgt gatggcctgg
     4321 ccgagcacgc ccaggccgac gatgggggca atcggggcgt ttgtcagcgt ggccacggcc
     4381 agcacgaaga cctcgaagct gaggacgtac ggcacgaggc cgctcacgag cgccacgacg
     4441 aacgtcgtcg cgtacagggc gatcgccccc aggctgcgca gctgctcgaa atccactcgc
     4501 gtcaggccct ctcccggcag gtcggcccgc gggagcacca attctagccg gatgggcgcg
     4561 gccgggccag cgcggggcgc ggccggacag gggcgggcga acggctcgcc ggcggtgccc
     4621 tcgcctcccc tcgcgcgtgg gcctagcgtt caaccggctt gatgaccgtc gtcccggcga
     4681 aggccttctt caccgcctcg gcgtccgcct tcgacccgac gaggtcgccc cagtgcatcg
     4741 ggatgagcac cttcggcttg aagccgttgg ccgccgccgc catctccgcc gccgtcatgg
     4801 tgtaggtgcc gccgcacggc atcatcgcga cgtcgatgtt cgccagcgcg agcatctcgg
     4861 gaatcgcgtc cgtgtcgcct gagtggtaca cgcgcgagcc gtccgacagc gtgatgatgt
     4921 agccgagcca gccgctggcc ctggggtgga acggcttccc gacgttgtag gctggaacgg
     4981 cctccaccct gatcggaccc gccgcgaacg tcccgccgga agcggcgatg accacccgtc
     5041 tgcctttcag tttcgcggcg acgtcggcgg gcgccacgaa caccgtgccg ggctgctcga
     5101 tgcgcgcgat gtccccgggc gagaagtggt ccgagtggcc gtgcgtgacg agcacgactg
     5161 cggccttggg cgcgagcgcg ggcagcttcc acggatcgat gtagatctgg gccgccccgt
     5221 cctcgatgcg gaacgaggcg tgcccgagcc agtggatgtg atcgacggtc atgggcgcct
     5281 ccggcaggag cagccacagg caggcgaggg cgattgggcg ctggatcatg gcgcgcaccg
     5341 cgcgtgtcgg ggccggctgg ggccggcggc cctggacgct gcgccgatca tatcctcctt
     5401 cgcggcgggt tgagcgcagg cgcggggccg cacgagcggg cggacgccgg gttctccagg
     5461 ggggcgcccc attcgggggg cgcggggccg cgcgagtgga cgggcggggg gcattctgag
     5521 gtaaacaact cagtgcgccg ggttggccgt catgacgggg ggtgccatgt ccgcgagagt
     5581 cgcaccggtt ctggccgttc tgctgcttgc cgccgccgca ccgccgcggc tcgcgggcct
     5641 gtccgcgccc gcgcagtccc cgcccgccca atccccgccc gcgcagtccc cgcccgccca
     5701 atccccgccc gcgccggagt gggaggaccc ggggatcttc gccgtgggcg ccgagaagcc
     5761 gcgcgcggcg ttcgtgccgc acgccgaccg cggcggcgcg ctcgcgcgcg accggacgcg
     5821 ctcgccgtac ttccggctgc tgaacggcac ctggaagttc cgctgggcca agaacccgtt
     5881 ctcgctgccg aacggattcg agtcgcctgc ctacgatgac gcagactggg atccgatgcc
     5941 ggtgccgtcc aactggcagg tcgtgggcgc gaacgagggc cggccgtacg atcggccgtt
     6001 cttcaccaac atcaagcacc cgttcagggc cgacccgccg cgcgtgccgc acgacgacaa
     6061 ccccgtcggc ctgtaccgca cggcgttcga ggttcccgcg ggatggcgcg gccgctcggt
     6121 gttcgtccac ttcgcgggcg tgcagtcggc gtactacgtg tggctgaacg ggacgcggat
     6181 tggataccgc gaggacgcgt tcacgccggg cgagttcgat ctgacgccgc acctgcggcc
     6241 cggccggaac gtgctggcgg tcgaggtgct ccaccactcg gacggcagct atctcgagga
     6301 ccaggactac tggcggctgg ccggcatctt ccgcgacgtc tatctcgtgg cgcagcccgc
     6361 cgtgcgcctc cgggacttcc tggtgcgcac ggatctcgac gccgcgtacc gcgacgccgt
     6421 gctcgaggtg cgtgcgtcgc tcgagaaccg gtccggcgcc gccgcccgcg gccatgcgct
     6481 ccgcgcggca cttgtcgggc cggacggccg cgaggtcttt gccgcggatc tggccgggcc
     6541 ggcccagatc gcgccggacg ccgaggccga gctgcgcgcg tcgggggcgg tccgggcccc
     6601 ggcgctctgg acggccgaga cggccgcgct ctacacgctc gtcgtcgagc acctcgacgc
     6661 gtcgggcgcg gtcctcgagg ccgcgtcggc gcgcgtcggg ttcaggaagg tcgagatcag
     6721 gggggggcag ctgctgctga acggcgtgcc gatcacgttc cggggcgtga accggcacga
     6781 gttcgacccg gatcgcgggc gcgtggtgtc acgcgagcgc atgctcgagg acatccgcct
     6841 gatgaagcag ttcaacttca acgcggtccg cacgtcgcac taccccaacg accccctctg
     6901 gtacgacctc tgcgacgaac tgggcctcta cgtcgtggac gaggccaacg tcgagagcca
     6961 cgagctgtgg gagcggaagg tctacatcgg cgacgacccg gcgtggaccg ccgccttcgt
     7021 cgcgcgcggc atcgcgatgg tggagcggga caagaaccac ccgtcggtcg tctactggtc
     7081 gatggggaac gagaccggcc tcggccgcaa cttcgacgcg atgtacgcgg ccatgaaggc
     7141 cgtcgatccc acgcgcccga tccactacga gagccgcaac cctccctacg cgccgacgct
     7201 ctcgagctac gacatcatct cgacgatgta cccggcggtg gaccacatca ttgacctgat
     7261 gaaccaggac ccctcccgcc cggtgattat ctgcgagtac gcgcacgcga tggggaacag
     7321 cctggggaac ttcaagaagt actgggacgc ctacgacgcg tacccgcgcc tgcagggcgg
     7381 cttcatctgg gactgggtgg accaggcgct gcggcacccg ggcccggacg gccgcgtgcg
     7441 gtggaactgg gtgaacacga gcgacggctc caacgcgaac gacggcctcg tgaacgccga
     7501 ccggacgccg cagccggagc tcttcgaagc gaagaaggtg cagcagccgg tgaaggtgga
     7561 gggcgtcgat ctggcacgcg gccgggtgcg tgtcacgaac
//
LOCUS       DAOSIF010000090         7588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8912_length_7588_cov_3.865923, whole genome shotgun sequence.
ACCESSION   DAOSIF010000090 DAOSIF010000000
VERSION     DAOSIF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8912_length_7588_cov_3.865923"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1096
                     /gene="queA"
                     /locus_tag="PLT35_04600"
     CDS             50..1096
                     /gene="queA"
                     /locus_tag="PLT35_04600"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOG28539.1"
                     /translation="MRVADFAFDLPAELIAQYPPAARGASRLLVLDRQRGSVAHAAVP
                     DLGGLLRAGDLMVFNDTRVFPARLVGEREPGGGGVEVLLLSRVDDEHWEALVHPGQRM
                     RPGRRVRFERGGRAIHGEVLEERFFGRRLVRLWTRDAGTVTDAIEAVGHVPLPPYIKR
                     PDVDLDRERYQTIFAREPGSIAAPTAGLHFTEAILQSLDAAGVERTMLTLHVGYGTFK
                     PVRVEDADDHSVDPERYAISEGAASRINAALDAGRRIVAAGTTTTRALESAVALGDGR
                     VRPGPAETALFIRPGHRFRAVGALFTNFHLPGSSLLMLVAAFGGREQVLAAYGEAVRE
                     GYRFYSYGDAMLIV"
     gene            1217..2164
                     /locus_tag="PLT35_04605"
     CDS             1217..2164
                     /locus_tag="PLT35_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28540.1"
                     /translation="MPFPYEEFDASDIATYALAARASKVNVEAFARPHRKGADLAAFV
                     EGLPAILGAADFKAVVRAICAARASGGGIIWGFGAHVVKTGLAPVLIDLAERGFVSAF
                     ATNGAGIIHDFEIALAGSTSEDVDAALGPGRFGMADETGRLLNEIITDGVGRGMGLGQ
                     SVAAHLAGVKPPFAHASIACASARLGIPLTAHVALGTDIIHMHPAASGAAIGEGSLRD
                     FRYFTSNVAKLDHGVYLNCGSAVVLPEVFLKAVALARNRGIALAGLTTVNLDFLRMYR
                     PQTNVVTRPPAGTGGRGYSLVGHHEILIPLLAAALVESA"
     gene            complement(2179..2982)
                     /locus_tag="PLT35_04610"
     CDS             complement(2179..2982)
                     /locus_tag="PLT35_04610"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOG28541.1"
                     /translation="MRAAPPATPPRLRPPLKWAGGKRWLVPHVKRLWLGHEPRRFVEP
                     FCGGLAVALGLAPQRALLNDLNPHVINFYRHVKRGLVISLPMENARDAYYACRKLFNA
                     LLAAGRGHTAEAASLFYYLNRTGYNGLCRFNSRGEFNVPFGSFARIAYVRDFAAFRPA
                     FRRWQFASLDFEALRLRPGDFVYADPPYDVPFTQYARARFTWDDQVRAAEWLARHEGP
                     VVLSNQATPRVVALYARLGFRTRFLSAPRRISCTGDRSPAREVLATRNL"
     gene            3310..3386
                     /locus_tag="PLT35_04615"
     tRNA            3310..3386
                     /locus_tag="PLT35_04615"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3344..3346,aa:Met,seq:cat)
     gene            3464..4903
                     /gene="tilS"
                     /locus_tag="PLT35_04620"
     CDS             3464..4903
                     /gene="tilS"
                     /locus_tag="PLT35_04620"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOG28542.1"
                     /translation="MPLQDRVRRAVERAGLIPAGSRVLAAVSGGPDSVALLRLLLALA
                     DACGFTVAGVAHLNHRLRGAESDGDEAFCRALARAHGLAFSCGRRDVAALARATHASP
                     ETAARRARYAFLEQAAARLKAGVIATGHTRDDQAETFLLRLLRGAGASGLAGIPPKRG
                     PIVRPLLDVRRAEILAYLARLRQPFRTDSSNQDRAIPRNWVRHELIPMLARHVNGDIV
                     DVLAREAALLRDETELLDRLALDAGAIVERRGAGPTVALDAAALAGLPPALARRVVRR
                     ALGKTGSGRFHGFDHVEQVIDLARSATKGSRAADLPGVRVERNGAEVVLSERGPRPPR
                     RREAFWYRLAVPGRLAVPECGCVIEARARRWSAAELPLAKAVSAGGRRAVVAAKAAAG
                     GLAVRSRRPGDAFRPFGMRGRKKLQDVLTDRKVPRAERDRVPLVVDAADKILWVGGHI
                     LAEEARATARTRRVVVLSMRQSGRKGREA"
     gene            4900..6816
                     /gene="ftsH"
                     /locus_tag="PLT35_04625"
     CDS             4900..6816
                     /gene="ftsH"
                     /locus_tag="PLT35_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG28543.1"
                     /translation="MNSTLRSLLFWLVLAVVVVVVWNFQTTFSPRERSVPFTEFMAWV
                     ESGQVAEVVVAGNEVTGTTKSNETFRTYAPIQYEGLANKLVERGVVITAKPESASPWA
                     SILYTWAPALLMIGFWIFFMRQMQSGGNKALSFGKSKAKLSSGTQKKVTFKDVAGVDE
                     AKEELQEIIEFLKEPQRFQKLGGHIPKGVLLMGSPGTGKTLLARAVAGEANVPFFSIS
                     GSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFESNEGVILMAATNRPDVLDPALLRPGRFDRRVVVNRPDVRGREGILVV
                     HTRKIPLADDVDPSVLARGTAGFSGADLANLVNEAALNAARYNQKAVRMADFEFAKDK
                     VLMGSERRSMVISEAEKRVTAVHEAGHALLTVVLPGADPVHKVTIIPRGMALGLTQQL
                     PVDEKHNYSREYLADQIAILLGGRIAEEVCLGGITTGAGNDLERATELARRMVCEWGM
                     SEEMGPLTFGKKEEQIFLGREIAQHQDYSEDTAVKIDQEVRRIVQENYARAQGLLTSH
                     KDALQKIADELLTREVLDGAQVKAIVDGEPLDEPAPSRAAPAAPAEPPAADADRAAAA
                     AVPPPLAEALPQQR"
     gene            6966..>7588
                     /gene="folP"
                     /locus_tag="PLT35_04630"
     CDS             6966..>7588
                     /gene="folP"
                     /locus_tag="PLT35_04630"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG28544.1"
                     /translation="MPALVAPRARFALGLAHGRSLELGPRTLLMGILNVTPDSFAGGV
                     ADPDEAAAAALRMEAEGADIVDIGGESTRPGAGPVDAREELARVVPVIERLAGRLRVP
                     ISVDTSKAPVAEAAIRAGACLVNDISGLGYDANLGAVAASAGAGLVLMHTRGRPRDMY
                     REAVYGDVVAEVSAELAQALARAEAAGVDRARTVLDPVHRIARRQQG"
BASE COUNT         1055 a         2662 c         2830 g         1041 t
ORIGIN      
        1 tgaataacaa ttctcgttgt ttgcgaaaac cgtcccgacc ccaattgaga tgagggtcgc
       61 tgacttcgcc ttcgacctgc ccgccgaact catcgcgcag tacccgccgg cagcgcgggg
      121 ggcgtcgcgg ctgctcgtgc tcgaccggca gcgcggctcg gtggcccacg cggccgtgcc
      181 cgacctcggc ggcctgctcc gcgccggcga cctgatggtc ttcaacgaca cccgggtctt
      241 cccggcgcgg ctcgtcggcg aacgcgagcc cggcgggggc ggcgtcgagg tcctgctgct
      301 ctcgcgcgtt gacgacgagc actgggaggc gcttgtccac ccgggccagc ggatgcggcc
      361 gggccggcgc gtgcgcttcg agcggggcgg gcgcgcgatt cacggcgagg tgctggaaga
      421 gcgcttcttc ggccgccgcc tcgtccggct ctggacgcgc gacgcgggca cggtgacgga
      481 cgccatcgaa gccgtcggcc acgtcccgct gccgccctac atcaagcggc cggacgtgga
      541 cctcgatcgc gagcggtacc agacgatctt cgcgcgcgaa ccgggctcga tcgcagcccc
      601 caccgccggc ctgcatttca ccgaggccat cctccagtcg ctcgacgcgg cgggcgtcga
      661 gcgcacgatg ctgacgctgc acgtgggcta cggcacgttc aagccggtcc gggtcgagga
      721 cgcggacgac cactcggtgg atcccgagcg ctacgccatc agcgagggcg cggcctcgcg
      781 catcaacgcc gcgctcgacg ccggccgccg catcgtggcc gccggcacga cgacgacgag
      841 ggcgctcgag tcggctgtgg cgctggggga cgggcgcgtg cgccccggtc ccgccgagac
      901 cgccttgttc atccgcccgg ggcaccggtt ccgcgccgtc ggcgcgctgt tcaccaactt
      961 ccacctgccg ggctcgtcgc tgctcatgct cgtggcggca tttggcgggc gcgagcaggt
     1021 gctggccgcg tacggcgagg cggtgcgcga gggctaccgg ttctacagct atggcgacgc
     1081 catgctcatc gtgtgacacg cacgccgagc ggccgacagg cgcgccgcgg gccggagcag
     1141 gcggcgccct cggggcgccc atcgggatgc gttaggatcc tgccggcgga tcgcgcggtg
     1201 cgagcgagga gcagccatgc cgttcccgta cgaggagttc gacgcgtcgg acatcgcgac
     1261 ctacgcgctg gctgcacgcg ccagcaaggt caacgtcgaa gcgttcgcgc ggccccaccg
     1321 gaagggggcc gatctggcgg ccttcgtcga ggggctgccc gccattctcg gcgctgccga
     1381 tttcaaggcc gtcgtgcgcg ccatctgcgc ggcgcgcgcg tccggcggcg gaatcatctg
     1441 gggcttcggc gcgcacgtcg tcaagaccgg cctggccccg gtgctcatcg acctggcgga
     1501 gcgcggcttc gtctcggcgt tcgcgacgaa cggcgccggc atcatccatg acttcgagat
     1561 tgcgctcgcg ggttcgacct cggaggacgt cgacgccgcg ctgggccccg ggcgcttcgg
     1621 gatggcggac gaaaccgggc ggctcctcaa cgagatcatc acggacggcg tcggcagggg
     1681 catgggactc ggccagtccg tcgccgcgca cctcgccggc gtgaagccgc ccttcgcgca
     1741 cgcgagcatc gcctgcgcgt cggcgcgcct cggcattccg ctcacggcgc acgtggccct
     1801 cggcaccgac atcatccaca tgcacccggc ggcgtctggc gccgccatcg gcgagggcag
     1861 cctgcgcgac ttccggtact tcacgtcgaa cgtggcgaag ctcgatcacg gcgtctacct
     1921 caactgcgga tccgcggtgg tgctgcccga ggtcttcctc aaggccgtgg ccctcgctcg
     1981 gaaccggggc attgcgctgg ctggcctcac gacggtcaac ctggacttcc ttcggatgta
     2041 ccgtccgcag acgaacgtcg tcacgcgccc gccggccggg accggcggca ggggctactc
     2101 gctcgtcggc caccacgaga tcctgatccc gctgctcgcc gccgcgctcg tcgaatcggc
     2161 ctaggggcca ggccccttct acaggttccg cgtggcgagg acctcgcggg cgggggagcg
     2221 gtcgccggtg cagctgatgc gccgcggcgc gctgaggaag cgcgtccgga agccgaggcg
     2281 cgcgtagagc gcgacgacgc gcggcgtggc ctggttcgag agcacgaccg gcccttcgtg
     2341 ccgcgccagc cactcggccg cgcgcacctg gtcgtcccag gtgaagcgcg cccgcgcgta
     2401 ctgggtgaaa ggcacgtcgt agggcgggtc ggcgtacacg aagtcgccgg gccggaggcg
     2461 cagcgcctcg aaatcgaggc tggcgaactg ccagcgcctg aacgcggggc ggaaggccgc
     2521 gaagtcgcgg acgtaggcga tgcgcgcgaa gctgccgaag ggcacgttga actcgccccg
     2581 gctgttgaac cggcagagcc cgttgtaccc cgtccggttc aggtagtaga acagcgacgc
     2641 ggcctcggcg gtgtgccccc ggccggccgc aagcagcgcg ttgaacagct tccggcaggc
     2701 gtagtaggcg tcgcgcgcgt tctccatcgg caggctgatg acgaggccgc gcttgacgtg
     2761 gcggtagaag ttgatgacgt gcgggttgag gtcgttcagc agcgcgcgct gcggcgccag
     2821 cccgagggcc accgccaagc cgccgcagaa cggctccacg aaccggcgcg gctcgtgccc
     2881 gagccacagc cgcttgacgt gcggcacgag ccagcgtttg ccgcccgccc acttgagggg
     2941 cgggcgcagg cggggcggcg tggcgggcgg tgcggcacgc acgagcggca gtatacgcgc
     3001 gccggcgcag gatcgcgcgt cgctccggtc aggcggcccc gtcggcgagg ctcgttgcgt
     3061 cccacacgcg ctcccaatcc gcctggagct caggcggccc cgtcggcaag gctcggacaa
     3121 cgagcgcccc ggttcgcgct cctgacgatc gtcgggaggc ttcgacccgc ttccgcgacc
     3181 tccaggcggc gcaggccgcc gatctgaagc cggcgggcct gaccgaggcc gacctcgagg
     3241 cggaggcgcc gcgccggccg cggggccgtt tggcggacac ctggagggcc tgctaaaatg
     3301 ggacgttacg ccggtgtagc tcagctggtt agagcacgcg gctcatatcc gcggagtccg
     3361 gggttcaagt ccctgcaccg gcaccagctt ccagaggagg ggtcgcgccg caccgccacg
     3421 tcgggcccct ccccgccttt ccagcaggct tcccgtgccc acgatgccac tccaggatcg
     3481 cgtcaggcgc gccgtcgagc gcgccggcct gattccggct ggctctcgag tcctggcggc
     3541 tgtctcggga ggaccggact cggtcgccct gctgcgcctg ctgctcgcgc tggccgacgc
     3601 gtgcggcttc acggtggccg gcgtggcgca cctgaaccac cgcctgcgcg gcgcggagag
     3661 cgacggcgac gaggcgttct gccgggccct ggcccgggcc cacggcctgg ccttcagctg
     3721 cggccgccgc gacgtggcgg cgctggcgcg ggccacgcac gcgtcgcccg agactgccgc
     3781 ccgccgcgcg cgctacgcgt ttctcgagca ggccgcggcg cggctgaagg ccggcgtcat
     3841 tgccacgggg cacacgcggg acgaccaggc cgagacgttc ctcctccggc tcctccgagg
     3901 cgccggcgcc tccggcctgg ccggcattcc gccgaagcgc ggaccgatcg tcaggcccct
     3961 cctcgacgtg cgccgcgccg agatcctcgc gtacctcgcc cgactgcggc agccgttccg
     4021 caccgactcg tcgaaccagg atcgggccat cccgcgcaac tgggtccgcc acgaactcat
     4081 ccccatgctg gcccggcacg tcaacggcga catcgtggac gtccttgcga gggaggcggc
     4141 gctcctgcgc gacgaaaccg aactgctcga ccgcctggcc ctcgacgcgg gcgcgatcgt
     4201 cgaacgccgg ggggccgggc cgacggtggc gctcgacgcg gccgcgcttg ccggactgcc
     4261 gcccgcgctg gcgcggaggg tcgtccgccg ggcgctcggc aagacgggga gcggccggtt
     4321 tcacgggttc gaccacgtcg agcaggtcat cgatctcgcc aggagcgcca cgaagggctc
     4381 ccgcgcggcc gacctgccgg gcgtgcgcgt ggaacgaaac ggcgcagaag ttgtcttatc
     4441 tgagagaggg ccgcgcccgc cgcgccgccg ggaggctttc tggtaccggc tggcggtccc
     4501 agggcgcctt gccgttcccg agtgcggctg cgtgatcgag gctcgggccc ggcgctggtc
     4561 tgccgccgaa ctgcctcttg ccaaggcggt tagcgccgga ggccggcgcg cggtggtggc
     4621 ggcaaaggcc gcggcggggg gattggcggt ccgctcgagg cgtcctggcg acgccttccg
     4681 gcctttcggg atgaggggcc gcaagaagct gcaggacgtg ctcaccgaca ggaaggtgcc
     4741 gcgggcggag cgggaccggg tgccgcttgt cgtcgatgcc gccgataaga tcctctgggt
     4801 tggcgggcac atcctggccg aggaggcccg cgcgacggcg cggacgcggc gcgtggtagt
     4861 cttgagcatg aggcagtcgg gaaggaaggg acgcgaggcg tgaattccac cctcaggagc
     4921 ctgctgttct ggttggtgct ggccgtcgtg gtggtcgtgg tgtggaactt ccagaccacg
     4981 ttctcgccgc gggagcggtc ggtgccgttc acggagttca tggcctgggt cgagtcgggc
     5041 caggtggccg aggtggttgt cgcggggaac gaggtcacgg gcaccacgaa gtcgaacgag
     5101 accttccgca cctacgcccc gatccagtac gagggcctgg cgaacaagct cgtcgagcgc
     5161 ggcgtcgtca tcaccgccaa gcccgagtcg gcgagcccct gggcgtcgat cctctacacg
     5221 tgggcccccg ccctgctcat gatcgggttc tggatcttct tcatgcgcca gatgcagagc
     5281 ggcggcaaca aggcgctgtc gttcggcaag agcaaggcga agctgtcctc agggacccag
     5341 aagaaagtca cgttcaagga cgtggcgggc gtggacgagg ccaaggagga gctgcaggag
     5401 atcatcgagt tcctcaagga gccgcagcgc ttccagaagc tcggcggcca catcccgaag
     5461 ggcgtgctcc tgatgggctc gcccggcacg ggcaagacgc tgctggcgcg cgcggtggcc
     5521 ggcgaggcca acgtgccatt cttctcgatc agcggatcgg acttcgtcga gatgttcgtg
     5581 ggcgtcggcg cgtcgcgcgt gcgggacctc ttcgagcagg gcaagaagaa cgccccctgc
     5641 atcatcttca tcgacgagat cgacgcggtg gggcggcacc gcggcgccgg cctcggcggc
     5701 ggccacgacg agcgcgagca gacgctcaac cagctgctcg tcgagatgga cgggttcgag
     5761 tcgaacgaag gcgtcatcct gatggcggcc accaaccggc cggacgtcct cgatcccgcg
     5821 ctgctcaggc ccggccgctt cgaccggcgc gtggtcgtga accggccgga cgtgcggggc
     5881 cgcgagggga tcctcgtggt gcacacgcgg aagatcccgc tggccgacga cgtggacccg
     5941 agcgtgctgg cgaggggcac cgccgggttc tcgggcgccg acctggcgaa cctggtcaac
     6001 gaggcggccc tgaacgcggc caggtacaac cagaaggcgg tgcggatggc cgacttcgag
     6061 ttcgcgaagg acaaggtcct catgggctcg gagcggcgct cgatggtgat cagcgaggcc
     6121 gagaagcgcg tgacggcggt gcacgaggcc ggccacgcgc tgctgacggt ggtgctgccg
     6181 ggggccgatc cggtccacaa ggtgacgatc atcccgcgcg ggatggcgct gggcctcacg
     6241 cagcagctgc cggtggacga gaagcacaac tactcgcgcg agtacctcgc cgaccagatc
     6301 gcgatcctgc tcggcgggcg gatcgccgag gaggtgtgcc tgggcggcat cacgacgggg
     6361 gccggcaacg acctcgagcg cgcgacggag ctggccaggc ggatggtgtg cgagtggggc
     6421 atgagcgagg agatgggccc gctgaccttc ggcaagaagg aggagcagat cttcctcggc
     6481 cgcgagatcg cgcagcacca ggactacagc gaggacacgg cagtcaagat cgaccaggag
     6541 gtccggcgca tcgtccagga gaactacgcc cgcgcgcagg gcctgttgac gtcgcacaag
     6601 gacgcgctgc agaagatcgc cgacgagctg ctgacgcgcg aggtgctcga cggcgcccag
     6661 gtcaaggcca tcgtggacgg ggagcccctt gacgagcccg cgccatcgcg cgcggcgcca
     6721 gccgcgccgg ccgagccccc ggccgcggat gcggaccggg ccgccgcggc ggcggttcct
     6781 cccccgctgg cggaggcgct gccccagcag cgataggccc aggccgcgcg ccgcctccgc
     6841 gagggcccgc gcccgccggg ttctcgccgc gtgccgcggc gtccgcagcg gcgcggcggc
     6901 gccgccggcc gccccgcgaa tcacccgcct gagtccgcgc cgcttctgtc agaattgagg
     6961 gcgtcatgcc cgcgctcgtg gccccgcgcg cccgattcgc tctcggcctg gcccacggac
     7021 ggagcctcga gctcggcccg cggacgctgc tcatgggcat cctcaacgtc acgccggact
     7081 cgttcgccgg cggtgtggcc gacccggacg aggccgccgc cgcggccctg aggatggagg
     7141 cggagggcgc cgacatcgtc gacatcggcg gcgagtccac gcggccgggc gccggcccgg
     7201 tggacgcgcg cgaagagctc gcgcgcgtgg tgccggtcat cgaacggctg gcgggccggt
     7261 tgagggtgcc gatctcggtg gacacgtcca aggcgccggt ggccgaggcg gcgatccgcg
     7321 ccggggcctg cctcgtgaac gacatcagcg gtctggggta tgatgcgaac ctcggcgcgg
     7381 tggcggcctc cgccggggcc ggcctcgtcc tgatgcacac gcgcggcagg ccgcgcgaca
     7441 tgtatcgcga ggcggtctat ggcgacgtcg tcgccgaggt gtcggcggag ctggcgcagg
     7501 cgctggctcg ggccgaggcc gccggggtgg accgcgcgcg caccgttctc gatcctgtcc
     7561 atcggatcgc tcgacggcag caaggcca
//
LOCUS       DAOSIF010000091         7583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_8920_length_7583_cov_4.116631, whole genome shotgun sequence.
ACCESSION   DAOSIF010000091 DAOSIF010000000
VERSION     DAOSIF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8920_length_7583_cov_4.116631"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1578)
                     /locus_tag="PLT35_04635"
     CDS             complement(100..1578)
                     /locus_tag="PLT35_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOG28545.1"
                     /translation="MTRRFTLPTVVLASALAFLVGVVLAGRMATLGPAAVRDGDPARA
                     AAGARPLSFADVAAKVNPAVVYIEAATSALTRRADGSQSGRQEAPSHDGDEAAEQAER
                     DSGSGFLLSSDGEILTNHHVVDGADRLMVTLADGRRLPARVLGADPDADLALLKLDGV
                     SGLPTVELGDSSTLRPGEWVCAIGNPLGYEHTVTVGVVSYLGRKLFDEGLDDYIQTDA
                     AIDVGNSGGPLIDASGRVVGINTAISAEGDGIGFAVPINQARDVLDHLRRRGRALRGY
                     MGVSLQGVDADLRRALGLGAVEGVLVEDVSANSPGERAGLRRYDVIESVEGRAVRTPS
                     AVIRDIASRLPGTQVRLRIVRDGRGQELTVSLVERPLRESPPERADRAAPAGPAHGRG
                     AGVLVEDLADAVRRRWKLPPDLAGVVIARVEPLSPADDADLQRGDVVLEVNRIPVRTA
                     ARYGQAVSAAAPQGALTLLLYDPRSGQRFIRTLRIGPQQEPD"
     gene            complement(1575..3581)
                     /gene="ligA"
                     /locus_tag="PLT35_04640"
     CDS             complement(1575..3581)
                     /gene="ligA"
                     /locus_tag="PLT35_04640"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG28546.1"
                     /translation="MTDAERAAELRRAIREHEERYYVLAAPTISDAEFDRLMADLRAL
                     EARRPDLVTADSPTQRVGGRPADGFATVTHAAPLLSLDNAYSTDELRAFHERTCRLAG
                     ADGRDAPITYVAELKIDGLSIALTYQDGALVRGVTRGDGVRGEDVTGNVRTIRAIPLR
                     LKGPAPGRLEVRGEVYLPRASFDRVNREREDADEAPFANPRNAAAGTMRSLDPAVVAR
                     RGLAAFFYQVAAGGGETGSHEDALRRMRGLGLPVEPHWAACDGIEAVIAYCAEWNGRR
                     GALGFDTDGVVVKVSDLALRARLGATNKFPRWAIAYKFPAQQARTRLKQIAVNVGRTG
                     AVTPYAILEPVSLAGSTIQMATLHNEQEIARRDIRPGDLVIIEKGGDVIPKVVAPVLD
                     ARPPDLLPWTMPSECPACGSRLARPDDEVVWRCLNGSCPARIRRSLLHFASRRAMDIE
                     GLGEALVDQLVEQGLVADVAGLYDLETPVLAGLSRMGPTSAKNLVERIARSRTVEFWR
                     VLYALGIRHIGERAAQTLAAAFGSMDALIAAPAEAMRAVRDVGPVAAEAARRFLDEPH
                     NRELIERLRAAGVPMAGAGPAPAAGQPLAGRTFVLTGTLDTMSRDEAERAITALGGRV
                     TGSVSRKTSYLVSGADPGSKLAKARDLGVPVLDEAAFARLIMNP"
     gene            complement(3628..4617)
                     /locus_tag="PLT35_04645"
     CDS             complement(3628..4617)
                     /locus_tag="PLT35_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG28547.1"
                     /translation="MDKRTLRLSDIFKPIQDDLVKVDEEFARQVESPVELIPRIGKYI
                     QSSGGKRIRPAVLLTSARLCGYQGDRAVLYAAVVEFVHAATLVHDDIVDNSPLRRGRT
                     AVHSQWGNDVSVLVGDYLYIKSMALALTHNELDVVRLLGDVTLRMIEGELYQLTKNGD
                     PDVTEEEHLEIIRRKTAYLFGACGRIGAMLAGQPPDRVEALGEYGFKLGVAFQIVDDL
                     LDLTGTVQSLGKPVGGDLREGKLTLPVIALMQRGGPRARSLVSEVLRDRALSSEQWQA
                     ISALLAEHRTIDYAFDRAVQFATEAKAHLLAFPPGEARDALAALPDYVLRRDR"
     gene            complement(4713..5492)
                     /locus_tag="PLT35_04650"
     CDS             complement(4713..5492)
                     /locus_tag="PLT35_04650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG28548.1"
                     /translation="MIDSHAHLADEAFVPDFDAVLSRARAAGLEAILCVVDATDAEET
                     ARADRLAPRWDGIRTTAGVHPHRAGAFAARPDAAKDLVAARLAADPAARAIGEIGLDY
                     HYDLAPRGVQRDVFAAQVALARETGRPVVIHTREADEDTMAILRREGGGCVRGVFHCF
                     SGDRRLAVGAVELGFHVSFSGLLTFPKAWTIRDAAAAVPLERLLVETDAPYLAPVPHR
                     GRRNEPAWVALTLQALAGLRGLDRLALEDRVRLNYRALVAP"
     gene            complement(5489..6778)
                     /gene="larC"
                     /locus_tag="PLT35_04655"
     CDS             complement(5489..6778)
                     /gene="larC"
                     /locus_tag="PLT35_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOG28549.1"
                     /translation="MTVVRMGSVLYFDCFSGASGDMVLGALIDLGVPLSGLRQALAGL
                     LPEGCELHAERVTRSGISATRFQVVDAHAHHGAHAHRDPAPDEYGERGAHSRREAHPH
                     HGHEPHRGLGEILGMIGASELPERVKAQASRLFERLARVEADIHQVPVERVHFHEVGA
                     LDSIVDIVGAVWAIDALRADRVVSSPLNTGGGTVETAHGLLPVPAPATLRLLEGVPAY
                     GADVQAELVTPTGALLVTGHAAAYGPMPRMVVGKIGYGAGQRDLPGRPNLLRVALGEE
                     AGVADDGRILSIECNLDDMNPQFLGGLFERLQASGALDVFFTPVQMKKNRPGTLVTVL
                     APPPLREAVVGVLFRETTTIGVRYHEVERECLLRDWRTVTTRFGPVRIKIARRGAAVM
                     NAAPEYEDCAALAESAGVAVREVHQAALHAYLEAEAT"
     gene            complement(6775..7461)
                     /gene="radC"
                     /locus_tag="PLT35_04660"
     CDS             complement(6775..7461)
                     /gene="radC"
                     /locus_tag="PLT35_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOG28550.1"
                     /translation="MSEDGTARAAGRDRPREKLSRLGAPALGDNELLALVLGYGSSGT
                     SALGLANLVLQASGGVHGLPGCTAEELRRVPGFGQARAAQVLASVELGRRTLLRQPAD
                     RPRLRTPGEVATYLLPEFGARPVEQFGVVLLDAKHRLLKTVLLTVGTLDRSVVHPREV
                     FREAVSSRAAGIVLFHNHPSGDPEPSADDLALTRRMVAAGELMGIDVLDHVVLTATRY
                     ASVRELGFLR"
BASE COUNT         1082 a         2755 c         2688 g         1058 t
ORIGIN      
        1 ggcgaactcg tatccctcgt actccaggat catccgcatc gaatcgcggt tggcctcttc
       61 gtcgtcgatg accaggatgc gcggtttcat gatctcccgt cagtccggtt cctgctgcgg
      121 gccgatcctg agggtgcgga tgaagcgctg gcccgagcgc gggtcgtaga gcagcaaggt
      181 gagggctccc tgcggcgccg ccgccgacac ggcctggccg tagcgggcgg cggtccgcac
      241 ggggatgcga ttgacttcca gcacgacgtc gccccgctgc aaatcggcat cgtcggcggg
      301 gctgagcggt tcgacgcgcg cgattacgac gcccgcgagg tcgggcggga gcttccagcg
      361 ccgcctgaca gcgtcagcga ggtcctcgac caggacgccg gcgccccggc cgtgcgccgg
      421 gcccgccggc gcggcccggt ccgcgcgctc aggcggactc tcgcgcaggg gccgctcgac
      481 gagcgacacg gtcagttcct ggccgcgccc gtccctgacg atccgcagcc ggacctgcgt
      541 gccgggcagc cgggacgcga tgtcgcgaat cacggctgac ggcgtccgga ccgctcggcc
      601 ctccacggat tcgatcacgt cgtatcgccg gagccccgcg cgctcgcccg gcgagttggc
      661 cgacacgtcc tccacgagga cgccctcgac ggcgcccagc ccgagcgcgc gccgcaggtc
      721 ggcgtcgacg ccctgcaggc tgacgcccat gtagccgcgc agggcccggc cgcggcgtct
      781 caggtgatcc agcacgtcgc gcgcctggtt gatcggcacg gcgaagccga tgccgtctcc
      841 ttccgcgctg atcgccgtat tgatcccaac cacgcgcccc gaggcgtcga tgagcggacc
      901 gccgctgttg ccgacatcga tggccgcatc ggtctggatg tagtcgtcga ggccttcgtc
      961 gaagagtttt cggccgaggt agctgacgac gcccaccgtc acggtgtgct cgtacccgag
     1021 gggattgccg atggcgcaca cccactcgcc ggggcggagc gtcgaggagt cgcccagctc
     1081 gacggtcggc aggccggaca cgccatccag cttcagcaaa gccaggtccg cgtcgggatc
     1141 cgcaccgagc acccgcgcgg ggagcctccg cccgtcggcg agcgtcacca tgaggcgatc
     1201 ggcgccgtcc acgacgtggt ggttcgtcag aatctcgccg tctgagctca ggaggaaccc
     1261 gctgcccgag tcgcgttccg cctgctcggc cgcctcgtcg ccgtcgtggg acggcgcctc
     1321 ttggcggccg ctctggctcc catccgcccg gcgcgtgagc gccgaggtcg ccgcctcgat
     1381 gtaaacgacg gccgggttga ccttcgccgc gacgtcggcg aagctgaggg gccgcgcgcc
     1441 cgccgccgct cgcgccggat cgccgtcgcg gaccgccgct gggccgagcg tcgccatccg
     1501 accggccaac acgaccccca cgagaaaggc gagggccgag gccaagacga cggtcggcag
     1561 cgtgaagcgg cgcgtcacgg gttcattata aggcgcgcaa acgctgcctc atcgaggacc
     1621 gggacgccga ggtcccgcgc cttggccagc ttgctccctg ggtccgcccc tgagacgagg
     1681 tagctcgtct tcctggagac cgaacccgtg acgcggcccc cgagggctgt gatggcccgt
     1741 tccgcctcgt cgcggctcat cgtgtccagc gtgcccgtca gcacgaacgt ccggccggcc
     1801 agcggctggc ccgccgccgg cgcgggcccg gcgcccgcca tggggacgcc ggcggcccgg
     1861 aggcgttcga tgagctcgcg gttgtgcggc tcgtccagga agcgccgcgc cgcctcggct
     1921 gccaccggcc cgacgtcgcg cacggcccgc atggcctcgg ccggcgcggc gatgagggcg
     1981 tccatcgatc cgaaggccgc ggccagcgtc tgcgcggcgc gttcgccaat atggcggatg
     2041 ccgagggcgt agagcacgcg ccagaactcg acggttctgc tccgggcgat gcgctcgacg
     2101 aggttcttcg ccgaagtggg ccccatccgg ctcaagcccg ccaagaccgg ggtctcgagg
     2161 tcgtacagcc cggcgacatc ggcgacgagg ccctgctcga cgagctgatc gacgagggcc
     2221 tcgcccaggc cctcgatgtc catcgcccgg cgggaggcga agtgcaggag gctgcggcgg
     2281 atccgggccg ggcacgagcc gttgaggcac cgccagacca cttcgtcgtc cggtctggcg
     2341 agccggcttc cgcacgccgg gcactcgctc ggcatcgtcc aggggagcag gtcgggcggg
     2401 cgggcgtcga gaaccggagc cacgaccttc gggatgacat cgcccccctt ttcgatgatg
     2461 acgagatccc cgggccggat gtcgcggcgc gcgatttcct gttcgttgtg cagcgtggcc
     2521 atctggattg tcgagccggc cagcgatacc ggctccagga tggcgtacgg cgtgacggcg
     2581 cccgtgcggc ccacgttgac ggcaatctgc ttcaggcgcg tgcgcgcctg ctgggcgggg
     2641 aacttgtagg cgatcgccca ccgggggaac ttgttggtcg cgccgagccg cgcgcgcagc
     2701 gcgaggtcgc tcaccttgac gacgaccccg tccgtgtcga acccgagcgc gccccggcgc
     2761 ccattccatt ccgcgcaata ggcgatgacg gcttcgatgc cgtcgcacgc cgcccagtgc
     2821 ggctcaaccg ggaggcccag gccgcgcatg cgccgcagcg catcctcgtg cgagcccgtc
     2881 tcgccgcctc cggccgccac ctggtagaag aacgcggcga gcccccgcct cgcgaccacg
     2941 gccggatcca ggctccgcat cgtgccggct gccgcgttcc gggggttggc gaagggcgcc
     3001 tcgtcggcgt cctcgcgctc gcggttgacg cggtcgaacg acgcccgcgg cagatacacc
     3061 tcgccccgca cctcgagccg gcccggtgcc ggccccttca ggcggagggg gatcgcgcga
     3121 atcgtccgca cgttgcccgt gacatcctcg ccgcggacgc cgtcgcccct cgtcacgccg
     3181 cgcacgagcg cgccgtcctg gtaggtcagc gcgatgctca ggccgtcgat cttcagctcg
     3241 gccacgtagg tgatcggcgc gtctctcccg tccgcgcccg ccaggcggca cgtccgctca
     3301 tgaaaggccc gcagctcatc ggtcgagtag gcgttgtcga ggctgagcag gggcgcggca
     3361 tgagtgacgg tggcgaagcc gtcggccggc cggccgccga cgcgctgggt cggcgagtcc
     3421 gccgtgacca ggtcggggcg gcgggcctcg agggcgcgca ggtccgccat gagacggtcg
     3481 aactccgcgt cggagatcgt cggagcggcc agcacgtagt agcgttcttc gtgttcgcgg
     3541 atggcgcggc gcagctccgc ggcacgttcg gcgtcggtca tcggggtcct cgcgggccgg
     3601 gcgcgctgcc ggcgcacgcg gcgcggatca gcggtcccgt ctcagcacgt aatccggcag
     3661 cgcggccaac gcatcccggg cctcgccggg cgggaacgcc aagaggtggg ccttggcttc
     3721 cgtcgcgaac tgcaccgccc gatcgaaggc gtagtcgatc gtccggtgct cggccagcaa
     3781 cgcactgatc gcctgccact gctcggagga gagcgcgcgg tcccgcagga cctcggaaac
     3841 cagcgaacgc gcgcgcggac cgccgcgctg catcagggcg atgacgggca gcgtcagctt
     3901 gccttcgcgc aggtccccgc ccacgggctt gccgagtgac tggacggtgc ccgtcaagtc
     3961 gagcaggtcg tcgacgatct ggaaggccac gccgagcttg aagccgtact cgccgagggc
     4021 ctcgacccgg tccggaggct gtcccgccag catcgcgccg atgcggccgc aggcgccgaa
     4081 caggtacgcc gtcttgcgcc ggatgatctc caggtgctcc tcctcggtca cgtccgggtc
     4141 gccgttcttc gtcaactggt acagctcgcc ttcgatcatg cggagcgtca cgtcaccgag
     4201 caggcgcacg acatcgagtt cgttgtgcgt cagggcgagg gccatcgact tgatgtaaag
     4261 gtagtccccc accagcaccg acacgtcgtt gccccactgc gagtgcaccg cggtgcgccc
     4321 tcgccgcagc ggcgagttgt ccacgatgtc gtcgtggacg agcgtggcgg cgtgaacgaa
     4381 ctcgacgacg gcggcgtaga gcacggcgcg atcgccctgg tagccgcaaa gccgcgccga
     4441 agtgagcagc accgccggcc ggatgcgttt gccgccgctc gactggatgt acttcccgat
     4501 cctgggaatc agttcgacgg gggattccac ctggcgggcg aactcctcgt cgaccttgac
     4561 gaggtcgtcc tggatcggtt tgaagatgtc cgagagtcgc agggtcctct tgtccacggc
     4621 accaggcggc aacaagccat cagaccacaa tcacccgggg ctgtcaacgc ggcaaagtgc
     4681 ctagcgacaa caggttcgcc gcgatcaggc attcacggcg cgaccagcgc gcggtaattg
     4741 agacgcacgc gatcctccag cgcgagccgg tcgaggccgc gcaggccggc caacgcctgg
     4801 agcgtcagcg cgacccaggc gggctcgttc cgccggccgc ggtgcgggac gggcgccagg
     4861 tagggcgcgt cggtctcgac aaggaggcgc tccaggggca ccgcggcggc ggcgtcgcgg
     4921 atcgtccagg ccttcggaaa ggtcaggagg cccgaaaagg acacgtgaaa gccgagctcg
     4981 acggcgccca cggccaggcg gcggtcgccg gagaagcagt ggaagacgcc gcgcacgcat
     5041 ccgccgcctt ccctgcggag gatggccatc gtgtcctcgt cggcctcgcg cgtgtggatg
     5101 acgaccggac ggcccgtctc gcgggccagg gccacctgcg cggcgaaaac gtcacgctgg
     5161 acgccgcgcg gcgcgaggtc gtagtggtaa tcgaggccga tttcgccaat cgccctcgcg
     5221 gccgggtccg cggcgagccg cgccgcgacg agatccttgg cggcgtccgg cctggcggcg
     5281 aaggccccgg cgcggtgcgg atggacgccg gcggtggtcc gaatcccgtc ccagcgcggt
     5341 gcgaggcggt cggctcgcgc cgtttcttcc gcgtcggtcg cgtcaaccac gcagaggatc
     5401 gcctcgagcc cggccgcgcg cgcgcgcgac agcaccgcgt cgaagtcagg gacgaacgcc
     5461 tcgtcggcca gatgcgcgtg cgagtcaatc acgtcgcctc cgcctccaga tacgcatgga
     5521 gcgcggcctg gtgcacctcg cgcactgcca ccccggcgga ttcggccagc gccgcgcagt
     5581 cctcgtactc gggagcggcg ttcatcacgg ccgcgccgcg gcgcgcgatc ttgatgcgta
     5641 ccgggccgaa ccgcgtcgtg acggtcctcc agtcccgcag caggcactcc cgctcgactt
     5701 cgtggtagcg aacgccgatc gtcgtcgtct cgcgaaagag cacgccgacc accgcctcgc
     5761 gcagcggcgg aggcgccagg acggtcacca gcgtgccggg ccggttcttc ttcatttgga
     5821 cgggcgtgaa gaacacgtcc agcgcgccgg acgcctgcag ccgctcgaac agcccgccca
     5881 ggaactgcgg gttcatgtcg tcgaggttgc actcgatcga cagaatccgg ccgtcgtcgg
     5941 cgacgcccgc ctcctcgccg agcgccacgc gcagcaggtt gggccgcccc ggcaggtcgc
     6001 gctggccggc gccgtacccg atcttgccga caaccatccg cggcatcggg ccgtacgcag
     6061 ccgcgtgccc cgtgacgagc agcgcgccgg tcggcgtcac cagctccgcc tgcacgtccg
     6121 cgccatacgc ggggacgccc tcgagcaggc gcagcgtggc aggcgccggc accggcagca
     6181 gcccgtgcgc ggtctcgacc gtgcctcctc ctgtgttgag cggggaggag accacccggt
     6241 ccgcgcggag ggcgtcgatg gcccaaacgg cgcccacgat atcgacgatc gaatcgaggg
     6301 cgcccacctc gtggaagtgc acgcgctcga cgggcacctg gtggatgtcc gcttcgacgc
     6361 gcgcgaggcg ctcgaacagc cggctcgcct gcgccttgac gcgctccggg agctccgagg
     6421 cgccgatcat tcccaggatc tcgccgaggc cccggtgggg ctcgtgcccg tgatgcgggt
     6481 gcgcctcgcg ccgcgaatgc gcgccgcgct cgccgtactc gtcaggcgcg ggatcgcgat
     6541 gcgcgtgtgc gccgtgatgc gcgtgcgcgt cgaccacctg gaaccgcgtc gccgaaatgc
     6601 ccgagcgcgt cacccgctcg gcgtgcagct cgcacccctc cggcaggagc ccggcgaggg
     6661 cctgccggag tccggacaac gggacgccca ggtcgatgag cgctccgagc accatgtccc
     6721 cggaggcccc cgagaagcaa tcgaagtaca gcacactgcc catgcgcacc accgtcatct
     6781 cagaaagccg agttcgcgaa cgctcgcgta gcgcgtggct gtcagcacca cgtgatcgag
     6841 cacgtcgatt cccatcagct cccctgccgc gaccatccgc ctcgtcaagg cgaggtcgtc
     6901 ggcgctcggc tcgggatcgc cggagggatg attatggaac aagacgatgc cggccgcacg
     6961 cgaggagacc gcttccctga agacctctct cgggtgcacg acgctccgat ccagcgtgcc
     7021 gacggtcagc agcaccgtct tcagcagccg gtgcttggcg tccaggagca cgacgccgaa
     7081 ctgctcgacc ggacgggcgc cgaattccgg gaggaggtac gtcgctacct cgcccggcgt
     7141 cctcagccgc ggccggtcag caggctgccg cagcagggtc cgccggccga gttcgacgga
     7201 ggcgagcacc tgggccgccc tggcctgccc gaatccgggc accctgcgca gctcctcagc
     7261 cgtgcagccg ggcaggccgt gcacgccgcc cgacgcctgc agcacgaggt tggcgagccc
     7321 gagcgcactc gtgcccgagg acccgtagcc cagcacgagg gcgagcagtt cgttgtcacc
     7381 cagggctggc gcgccgaggc ggctcagctt ctcgcggggc ctgtcgcggc ccgcggcgcg
     7441 ggccgtgccg tcttcggaca tcaccgtgtc tcctcgaacg gccggggtcc tccgctgctg
     7501 cgagcggggc ccggatgagc cgatgcaagc ggcgtgctgg tttcccggga gccgggttcg
     7561 caacgcggcg tggacaggcc gga
//
LOCUS       DAOSIF010000092         7475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9067_length_7475_cov_4.338544, whole genome shotgun sequence.
ACCESSION   DAOSIF010000092 DAOSIF010000000
VERSION     DAOSIF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9067_length_7475_cov_4.338544"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PLT35_04665"
     CDS             <1..1309
                     /locus_tag="PLT35_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG28551.1"
                     /translation="RDGRPTFASSLMLHAFRLTRPGEEVTFKHLREAAGEPASQVPAA
                     PDAALGDAGWWLSSLKHAPKTGLQRVHEAFPDRMRGMAAEAARASDVFTEFDGLAPSA
                     AQVLDPRTSGRPVSPTSLEKLAACPFAYFLKAGLRLDPIQEDERDLDAWLDPLTRGSL
                     LHELFAQTMRHLREAKEKPGLKRHLAWLKARADAALAALGETMPPPSDEVFNRERDDI
                     YRDLEVFLGTEELAAKEDGITPVGFEVGFAGYGQAGDEPLGQAEPVAIRLPGARFPLN
                     GRIDRIDRLRDGSYQVVDYKTGTLCWAGYSGVFRHGRLLQHALYAVAAESLLRAKQDP
                     SAKVTSGRYRFPTVKGAGRSKVIPRPPDRDLGRVVAAVLDIAAAGAFMARTEAKDKKA
                     CEYCDFAGTCEGAPVVARAAAKLDNPANEALEPWRRLQGEENA"
     gene            1302..4532
                     /locus_tag="PLT35_04670"
     CDS             1302..4532
                     /locus_tag="PLT35_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG28552.1"
                     /translation="MHKHVIPPDHAERERITADLATNMLVEAGAGSGKTESLAQRMVA
                     GILAGYGVSQMAAVTFTRKAAAELRGRFQIALEEALARETGPTANPEARERLTAALAG
                     LESFFAGTIHSFYARLLRERPVEAGLAPGFTEVEETEDAELRKQAWREYLARERGKGS
                     ALLQALADADVRPRDLDYAFAFVCTFEEVEFPPGEAPLPDTAKAWKALEKFLRSLERR
                     LPGPIADDTTCKVQQRLRDDVPRLGYARRDRPGDLVDLLRRWEANVSITQKCWSDDPA
                     EKKRLAAEISAIIEDFKSGTVTPFLAEWRQYVYRLAMPLLTDARAFAREARFRALQLN
                     YNDLLQGAARLLRDHPEVRRSLQQKYRWLYVDEFQDTDPVQAEVMLWLASDPSTPPSA
                     SDPFAVRLRPGSLFIVGDPKQSIYRFRRADIEVYNRVREAIVREHGCVVPLVASWRSV
                     SAICTWVSDAFEHVFPEAPTAHQAKYEALKPVVKKPGGGVVTLTHPETLKREEVFGAD
                     AAAIARYIRAECDAGRRRPGDFLILTWKRKHLGAYAAALEALEVPAEVTGAGAFGDSA
                     EVRALAVLLRVLGDPDDGGSVVGVLRSLVFGVSDDELFQHRHAGGWFTPQRRLEDGTS
                     QPGHPRVLAALYALGDMLELVRELPAPAAVERILDTTGLLAQAAAASPGGARAGDLLH
                     AVDRVREAFERGGTLADAALALQDDVDAAEVESWPLEPGRTDVVRVMNLHKAKGLEAP
                     VVFLADPCGGFKNDAELRIDRGPSGAVGHMQVVWKDEEDTWRRKTIAEPENWAEYAAV
                     EQPFLNAEAERLRYVAATRARELLVISRWEKTGGAQPWLPFEPFLAGADELEVPPKAA
                     PPAARKADLSEKTRTRLAADREVLLKEAMAHSYAVVSVTASTHQEAPPGEQEGLESGP
                     ATGAAFGDLVHRLLEHAMRRGTDAAAIERYANWLTFEKPDLQAAVPEALAAVRRVTAS
                     GVWEEALAAEARDVEVPFTLATAGADGTPTLLNGVIDLAYRTADGWVIVDYKTDQLGG
                     RPAAFLLDRYGAQIDAYCRAWAELTGTPPARAGIHAVRSGETVWRR"
     gene            complement(4762..5154)
                     /locus_tag="PLT35_04675"
     CDS             complement(4762..5154)
                     /locus_tag="PLT35_04675"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG28553.1"
                     /translation="MTDGSYARRYPASLFPGIHAVRLMPLGASATLVNLSATGILVES
                     EARLAPGAPVRIDFEGTFSPSSIESRVTRSEVARIHPDGSLRFHLGLAFGDRIALSID
                     APAPADAPGAAASAAASGAQVPALRNRW"
     gene            complement(5151..5807)
                     /locus_tag="PLT35_04680"
     CDS             complement(5151..5807)
                     /locus_tag="PLT35_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG28554.1"
                     /translation="MTKPDIDPSAEAAHLRRLLDTQPGCLMRLADDGTLLALNNSALA
                     LLGLTSVAQALGRDFSTWIPADERLQWTEFLFGVAQGNPASLECHVMPASGGLQPALL
                     HGVPIAQHPDGISSIAVAARGVAAQRQLQSAVLELRQQAERERAARARVEAQLAESEG
                     ARRAAETKLNAALTDVQQLEAALESFAARRRQPDGGPARADGTAALDAESEGPGGAHS
                     "
     gene            5979..6734
                     /locus_tag="PLT35_04685"
     CDS             5979..6734
                     /locus_tag="PLT35_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOG28555.1"
                     /translation="MESKLAEALRLASQPVAVFLADEKPDGAGQFAEGGWGCVAASMV
                     AVAKGRTAAFDRRTFGCPGGGTGLGFGNQYERTGFAIEDLLSTGSPERAARLRRRSRM
                     GEGERFFASPELVGRWLGTLPFTDVPAEYVVMRPLGQVAEAERPEVVVFLVNPDQLSA
                     LVTLSDFRRGSGRSVVARFGGACQSILFAYAEAGREQPRGVIGFFDIAQRARIDRDLL
                     SFTAPWALFLEMESNVKGSFLEMKDWLELRARI"
     gene            complement(6731..7021)
                     /locus_tag="PLT35_04690"
     CDS             complement(6731..7021)
                     /locus_tag="PLT35_04690"
                     /inference="COORDINATES: protein motif:HMM:NF039479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HOG28556.1"
                     /translation="MRSGARSRSGPASAHLGGDPGWPGSESQSPHPRGVSSQRRAGQA
                     TVVRMLVEYRVIDSTGRSTLVWIGLVMAGIVPGMGLSWPLVRARIVGQIEVD"
     gene            7079..>7475
                     /locus_tag="PLT35_04695"
     CDS             7079..>7475
                     /locus_tag="PLT35_04695"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOG28557.1"
                     /translation="MSMRNHVVAAAVALSLGLGASAAAQPANTLTPAEKKQGWVLLFN
                     GATFDGWRQVNGTAMPANWTIEDQAMKVFTGAGKKPGQGSGGDVIYAARKFRDFELSI
                     DWKTEKAGNSGIFFNVREVPGQAIYYAAPE"
BASE COUNT         1108 a         2597 c         2774 g          996 t
ORIGIN      
        1 gcgcgacggg cgtccgacct tcgcctcgtc tctcatgctg cacgccttcc gcctcacgcg
       61 gccgggcgag gaggtcacct tcaagcacct ccgcgaggcg gccggcgagc cggcctcgca
      121 ggtgcccgcc gcgccggatg cggcgctcgg cgacgcgggc tggtggctct ccagcctgaa
      181 gcacgcgccg aagacgggac tgcagcgcgt gcacgaggcc ttcccggaca ggatgcgcgg
      241 gatggcggcc gaggcagcgc gcgcctcgga cgtcttcacg gagttcgacg gcctggcgcc
      301 ctcggcggcg caggtgctcg acccgcgcac gtccggccgc ccggtctcgc cgacgagcct
      361 cgaaaagctg gcggcgtgcc cgttcgccta cttcctgaag gcgggcttga gactcgaccc
      421 gatccaggag gacgagcggg acctcgacgc ctggctcgac ccgctcacgc gcgggtcgct
      481 gctgcacgag ctgttcgcgc agacgatgcg gcacctgcgc gaggcgaagg agaagccggg
      541 cctgaagcgc cacctggcgt ggttgaaggc tcgggccgac gcggcgctcg cagcgctcgg
      601 cgagacgatg ccgccgccat cggacgaggt cttcaaccgc gagcgcgacg acatctaccg
      661 cgacctggag gtcttcctcg gaacggagga gctggcggcg aaggaggacg gcatcacgcc
      721 ggtcggcttc gaagtgggat tcgcgggcta cggccaggcg ggcgacgagc cgctcggcca
      781 ggcagaaccg gtggcgatcc gcctgccggg cgcccggttc ccgctcaacg ggcgcatcga
      841 ccgcatcgat cgcctgcgcg acggcagcta ccaggtggtg gactacaaga cgggcacgct
      901 ctgctgggcg gggtactccg gcgtgttcag gcacgggcgg ctcctgcagc acgccctgta
      961 cgcggtggcc gccgaatcgc tgctgcgggc gaagcaggat ccgtcggcga aggtgacgtc
     1021 gggccggtac cgcttcccga cggtgaaagg cgccggccgg tcgaaggtga tccctcgccc
     1081 gccggaccgc gacctcgggc gcgtggtcgc ggccgtcctc gacattgccg cggccggcgc
     1141 gttcatggcc aggaccgagg cgaaggacaa gaaggcgtgc gagtactgcg acttcgccgg
     1201 cacgtgcgag ggcgcgccgg tcgtcgcgcg ggcggcggcc aagctcgaca acccggcgaa
     1261 cgaggcgctc gagccctggc gccgcctcca gggggaagag aatgcataag cacgtcattc
     1321 ctcccgatca cgccgagcgc gagcggatca cggccgacct cgccacgaac atgctcgtcg
     1381 aagcgggcgc cgggtccggc aagacggaga gcctcgccca gcgcatggtg gcgggcatcc
     1441 tcgccggcta cggcgtgtcg cagatggcgg ccgtcacgtt cacgcgcaag gccgccgccg
     1501 agttgcgcgg ccgcttccag atcgccctcg aagaggccct ggctcgagag accggcccga
     1561 cggcgaaccc cgaggcgcgc gagcgactca ccgccgccct cgcgggcctc gagtcgttct
     1621 tcgccggcac gatccactcg ttctacgcgc ggctgctgcg cgagcggccg gtcgaggccg
     1681 ggctcgcccc gggcttcacc gaggtcgagg aaaccgaaga cgccgaactg aggaagcagg
     1741 cgtggcggga gtacctggcg cgcgagcgcg gcaaggggtc ggcgctcctg caggcgctgg
     1801 ccgacgccga cgtccggccc cgggacctcg actacgcctt cgccttcgtc tgcacgttcg
     1861 aggaggtgga gttcccgcca ggagaggccc cgctgccgga caccgcgaag gcgtggaagg
     1921 cgctggagaa gttcctccgt tcgctggagc gccgcctccc gggaccgatc gccgacgaca
     1981 cgacctgcaa agtgcagcag cgtctgcgcg acgatgtgcc gcggctgggg tacgcccggc
     2041 gtgaccgccc gggcgatctc gttgacctgc tgcgccgctg ggaagcgaac gtctcgatca
     2101 cccagaaatg ctggtccgac gatccggcgg agaagaagag gctggccgcc gagatttcgg
     2161 cgatcatcga ggacttcaag agcggcaccg tcacgccctt cctcgcggag tggcgccagt
     2221 acgtctaccg cctggcgatg ccgctgctga cggacgcgcg ggccttcgcg cgcgaggcgc
     2281 ggttcagggc tcttcagctg aactacaacg acctgctgca gggcgccgcg cggctgctgc
     2341 gcgatcaccc ggaggtgcgc cgctcgctcc agcagaagta ccggtggctc tacgtggacg
     2401 agttccagga caccgacccg gtccaggcgg aggtgatgct gtggctggcg agcgatccgt
     2461 ccacgccgcc ctccgcatcc gatccgttcg ccgtccgcct ccgcccgggc tccctcttca
     2521 tcgtcggcga cccgaagcag tcgatctacc gcttccggcg cgccgacatc gaggtctaca
     2581 accgcgtccg cgaggccatc gtccgcgagc acggatgcgt ggtcccgctg gtggcgtcgt
     2641 ggcggtcggt gtcggccatc tgcacctggg tcagcgacgc gttcgaacat gtgtttcccg
     2701 aagccccgac ggcgcaccag gcgaagtacg aagccctgaa gccggtcgtg aagaaacccg
     2761 gcggaggcgt ggtcacgctg acgcacccgg aaaccctcaa gagagaggag gtgttcgggg
     2821 cggacgccgc ggccatcgcg cgatacatcc gggccgagtg cgatgccggc aggcggcggc
     2881 cgggcgactt cctgatcctg acgtggaagc ggaagcacct cggcgcctac gcagccgcgc
     2941 tcgaggcgct ggaggtgccg gccgaggtca ccggggccgg ggccttcggg gattccgccg
     3001 aggtcagggc gctggccgtg ctgctgcgcg tgctcggcga tccggacgac ggcgggagcg
     3061 tcgtgggggt gctgcgcagc ctggtgttcg gtgtcagcga cgacgagctg ttccagcacc
     3121 gccacgccgg cggctggttc actccccagc ggaggctgga agacgggact tcgcagccgg
     3181 gccatccgcg cgtgctggcg gcgctttacg cgctgggcga catgctggag ctggtgcgcg
     3241 agctccccgc accggcggcc gtcgagcgca tcctcgacac gacgggcctg ctcgcgcagg
     3301 cggctgctgc gagcccgggc ggcgccaggg cgggcgacct gctgcacgcg gtggaccggg
     3361 tccgcgaggc gttcgagcgc ggcggcacgc tggccgatgc ggccctcgcc ctgcaggacg
     3421 acgttgacgc ggcggaggtg gaatcgtggc cgctcgagcc agggcggacc gacgtcgtgc
     3481 gggtcatgaa cctgcacaag gcgaaaggcc tcgaggcgcc cgtcgtcttc cttgcggacc
     3541 cgtgcggcgg cttcaagaac gacgcggagc ttcgcatcga tcgcggcccg tcaggggcgg
     3601 tcgggcacat gcaggtggta tggaaggacg aagaagacac gtggcgacgg aagaccatcg
     3661 cggagccaga gaactgggcc gagtacgcgg ctgtcgagca gccgttcctg aatgccgaag
     3721 ccgagcggct ccgctacgtt gcggcgacgc gggcgaggga gctgctcgtc atcagccggt
     3781 gggagaagac cggaggcgcc cagccgtggc tgccgttcga accgttcctg gctggagcgg
     3841 acgagctgga ggtcccgccg aaggcagcgc cgccggcggc acgaaaggcc gacctgagcg
     3901 agaagacgcg gacccggctg gccgccgacc gtgaggtcct gctgaaggag gccatggccc
     3961 actcgtacgc cgtggtatcg gtgaccgcct cgacgcacca ggaagcaccc ccgggcgaac
     4021 aggaagggct cgagagcggg cccgcaacgg gcgcggcatt cggcgacctg gtgcaccgcc
     4081 tgctcgagca cgccatgcgg cgagggaccg atgcggccgc catcgagcgg tacgccaact
     4141 ggctgacgtt cgagaagccg gacctgcagg cggcggtgcc ggaagccctg gcagccgttc
     4201 ggcgcgtgac cgcctccggc gtctgggagg aagcactcgc ggccgaggcg cgcgacgtcg
     4261 aggtgccgtt cacgctggcg acggcgggcg ccgacggcac cccgacgctg ctgaacggcg
     4321 tcatcgacct ggcctaccgt acggccgatg gctgggtgat cgtggactac aaaaccgacc
     4381 agctgggcgg acgtccagcg gcgttcctgc tggatcgcta cggggcccag atcgacgcgt
     4441 actgccgcgc gtgggcggaa ctgaccggca caccgcccgc gcgggcgggc atccacgccg
     4501 tccgctccgg cgagaccgtg tggagacgct gagagccggg ccgcgggccg cccggcgttc
     4561 cgctgccggc cggaacgccc gcgtcctctc gtgtcccgcg cccgcgccct accgtcctcc
     4621 gcccgagcct ctggagccgt cgcgtcagcg gactcctgca agcgggcgcc ttgcgcagcg
     4681 cgagggccga acccaaacgg acgaggccgg ctggcagagc gatcaggacg aggccgaacg
     4741 gcgcggcagc gcggcgtgcg gctaccagcg gttgcgaagc gcgggcacct gggcgccgct
     4801 ggcggcagcg ctggcggcgg cgcctggggc gtccgcggga gccggggcgt ctattgacag
     4861 cgcgatgcga tcgccgaacg ccagcccgag gtggaagcgc agcgacccgt cgggatggat
     4921 gcgcgcaacc tcgctgcgag tgacgcggct ctcgatcgag cttgggctga aggtgccttc
     4981 gaaatcgatc ctcaccggcg ccccaggcgc caggcgggct tccgactcga cgaggatgcc
     5041 cgtcgcggac aggttgacga gcgtggcgga cgcacccagg ggcatcagcc tgaccgcgtg
     5101 aatcccggga aagagcgaag cggggtaacg gcgcgcgtac gaaccgtccg tcatgagtgc
     5161 gctcccccgg gcccctcgct ctccgcgtcc agggccgctg tgccgtcggc gcgagcggga
     5221 cccccgtccg gctgtctccg ccgcgcggcg aaggactcga gagccgcctc gagctgctgg
     5281 acgtcggtca gcgcggcatt gagcttcgtc tcggcagcgc ggcgtgcgcc ctcggactcg
     5341 gcaagctgcg cctccacccg ggcccgcgcg gcgcgctccc gttcggcctg ctgcctcagc
     5401 tcgaggacgg cggactgcag ctgcctctgc gccgccacgc cgcgcgccgc cacggcaatc
     5461 gacgagattc catccgggtg ctgcgcgatc ggcacgccat gcagcagcgc gggctgaagg
     5521 ccgccggacg ccggcatgac gtggcactcc agggaggcgg ggttgccctg cgcgacgccg
     5581 aagaggaact cggtccactg caggcgttcg tcggccggta tccacgtgga gaaatcgcgc
     5641 ccgagcgcct gcgccaccga cgtcaggccc agcagcgcga gcgccgagtt gttgagggcc
     5701 agcagcgtcc cgtcgtcggc caggcgcatc aggcaccccg gctgggtgtc gagcaggcgg
     5761 cggaggtgcg cggcttccgc agagggatcg atgtcgggtt tagtcacgtt cactcacccg
     5821 ccgggcccgt ccccacggaa ccgggcccct gcacgccggc ctggccgagc gcggccacgc
     5881 cgggctcgtt ccgtcctcgt atcggctcgg attcccgcct ccagcagggc ccgctggagc
     5941 ccggtgtacg atgtctgtcc tggacggagg ttcgccgtat ggagagcaag ctggccgagg
     6001 ccctgcgcct cgcgtcgcaa cccgtggccg tcttcctcgc ggacgagaag ccggacggcg
     6061 ccgggcagtt cgcagagggc ggatggggct gcgtggcggc ctcgatggtc gcggtcgcga
     6121 aggggcggac ggcggccttc gaccgccgga ccttcggctg ccccggcggc ggcacggggc
     6181 tcggcttcgg caaccagtac gagcggacgg ggttcgcaat cgaggacctg ctctcgacgg
     6241 gcagccccga gcgcgccgcg cggcttcggc gccgctcacg gatgggcgaa ggcgagcggt
     6301 tcttcgcgtc gcccgagctg gtgggtcgct ggctcggcac gctgcccttc accgacgtgc
     6361 cggccgaata cgtggtcatg aggccgctcg ggcaggtggc cgaggccgag cggcccgagg
     6421 tggtggtgtt tctcgtcaat cccgatcagc tctcggcgct cgtgacgctc tccgacttcc
     6481 gccgcggctc aggcaggagc gtcgtcgcgc gcttcggcgg agcgtgccag tcgatcctgt
     6541 tcgcctacgc cgaggccggc cgggaacagc cgcgcggggt catcggtttc ttcgacatcg
     6601 cgcagcgcgc gcgcatcgac cgcgacctcc tctccttcac ggcgccgtgg gcgctcttcc
     6661 tggagatgga gtcgaacgtg aagggcagct tcctcgagat gaaggactgg ctggagctgc
     6721 gggccaggat ctagtccact tcgatctggc cgacgatcct cgcgcggacc agcggccacg
     6781 acaagcccat gccgggcacg atcccggcca tcaccagccc gatccagacc agcgtggaac
     6841 ggccggtcga gtcgatgacg cggtactcga cgagcatccg aacgacggtg gcctgtcctg
     6901 cgcgcctctg gctggagacg ccgcgcgggt gcggcgactg gctctccgaa cctggccagc
     6961 ccgggtcccc gccaaggtgg gccgaggcgg ggccgctgcg cgagcgagcc ccggatcgca
     7021 cgcgggtgcc ggggagtggt agagttcccc ggtcggcctg tcggagggga caaggaacat
     7081 gagcatgcgc aaccacgtcg tcgccgccgc tgtggcgctg agcctggggc tcggcgcgag
     7141 cgccgccgcc cagcccgcca acaccctcac gcccgcggag aagaagcagg gctgggtcct
     7201 gctgttcaac ggcgccacgt tcgacggctg gcggcaggtg aacggcaccg ccatgcccgc
     7261 caactggacg atcgaagacc aggccatgaa ggtgttcacg ggcgcgggca agaagccggg
     7321 ccagggctcg ggcggcgacg tcatctacgc cgccaggaag ttccgcgact tcgagctgtc
     7381 gatcgactgg aagaccgaga aggcgggcaa ctcaggcatc ttcttcaacg tgcgggaagt
     7441 gccgggtcag gctatctact acgcggcgcc cgagg
//
LOCUS       DAOSIF010000093         7419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9154_length_7419_cov_3.048343, whole genome shotgun sequence.
ACCESSION   DAOSIF010000093 DAOSIF010000000
VERSION     DAOSIF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9154_length_7419_cov_3.048343"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLT35_04700"
     CDS             <1..526
                     /locus_tag="PLT35_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28558.1"
                     /translation="VVPEADRAREPDIRAAARRALNRVAGALGVEPPAELDLVFHPTV
                     ESFARATGQPWWTAARTSGSRIDLLPPRVLEGRRTLESTLRHEFVHLLTEPVLAGRAL
                     WIREGLAVVVAGELPSAGADARGRGGARAAACPSDEDLRAPRDPDAWRRAYEAAGRCV
                     SEALAGGRRWQDLR"
     gene            complement(482..1243)
                     /locus_tag="PLT35_04705"
     CDS             complement(482..1243)
                     /locus_tag="PLT35_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28559.1"
                     /translation="MTTRTRIVLAWLSAGHAAVGGLYWLLLQVPESNAWMLLASALVA
                     IAAVLLLGWVEMAAALGFNAVMPMREAARLGARRAWLIVFPLAVFGCFWLVTADATGW
                     LEQNAGRIDAWVIARTGWTRTTGLHSAAGWIVAFVRYGIGVSLAAALLRALAVRGLRG
                     LGTGWVRSGLSGPRLIAVSAALMAGFWLPWHAAYWRPAWLPPTWVQPAFAAAKLLAIF
                     IIANAGWAFILRRAPRERAKPSAEILPAAAAGERL"
     gene            complement(1240..5952)
                     /locus_tag="PLT35_04710"
     CDS             complement(1240..5952)
                     /locus_tag="PLT35_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOG28560.1"
                     /translation="MTRHLLPCAAGLVFLAVAPASAQPAGDAGPAFHLWSSQVFTTTD
                     RPSFSLTYREVDHLDFRVYRVAEPFAFFASLPDPHQLGSEAPIVPQERSPIERIAAWK
                     AGQRKALRSFVRRQVSREYRAKRREARATRDVRRRVPLDYNSFAQVPLLNPSRLVASW
                     RELLPPVREPESRRIPLDVSAPGVYVVEAVGPPLKAYTIVIVSDVALVTKTAPGQALV
                     YAVDRGTGEPQAGCGLRTIVDGRPLEAATTGADGTALVAIAQPKPDTVIALATCEGQV
                     AVTDPGSWALQAPARELVGYLYTDKPVYRPGHTVRMKGVLRWRTAAGLEPFGGSDVEL
                     SVTDSNDKVVLRERRPVDAFGSVSSSFALPAGAALGGYVVTAASGDERASGSFEVQEY
                     RKPEFEVTVTAADRFVVQGGAVEAVFKARYYFGQPVAGGSVTYVVHRQPYYSPLRWSD
                     DGDDTGGWWGGGEESESVTVRLDDAGTAAVSIPLPVDDDGRDYTARIEARVTDASNRE
                     VAGHAFVHATWGRFLVVSDVDRYVQKPGSTAQVSIRAVDYEGVLQAGVALHLALERLK
                     YPQGRWNEPQVTIIASGTATTDEQGRASWSAALPQEPGSYRFRVSAESAGRRVEDTSG
                     VWVTGSADEFGGGDTYLELVADRASYAPGDTARLVVRGGAVSAPVLVTKEARQISFHR
                     VVRTGGEHAIEVPVSDADLGDTYVNIAYVKDDRLYRAEKRLKVPAVSRRLNVAVAAES
                     ATAKPREAGVFTLTVTDASGAPVRAQLSVGVIDEAVYGVKPDDTPDALRFFHRLSYSR
                     VSTDYSRDYSFVGYAGSHELQLAQRRRPYTLADFKQEGPPRPQVRKDFPDAIFWAADV
                     TTDAQGRATVRVPYPDALTTWRLTARAVTADTLVGAGLARTTTTKDLILRVVTPRFLT
                     EADRIDMPVIVHNYLPDTQAVKVTGEVEGLTFAGGDPSRATPQTVEVPAGAERALAWT
                     IDAPRAGAAVVSGTAAAAADADAIQLSFPVLPFGLQREEGRAGSMRGAGEQVVEVDVP
                     ASSNPSERAIRVQVAPSLGGPILAALDFLTSYPYGCTEQTVSSLLPNLVARRTLTDLQ
                     LPLTEGLKSLDRQVTEGLARLLDSQHEDGGWGWWKTDQNHPFMTAYAITGLLEARAAG
                     YKVEEWPIRRGVRALGALYAEYPAALPDLKAYETYVLLAAAAAGFEAPARGEGKGWSR
                     TAALDDLWSARDRMSAYGRSLLLLALDAAKDARGDALARDVASRAERKGDLAWWTSAN
                     DPLLFDFVDTSVEATALAAQALAARDPKHPLIEPAVRWLLLNRTFGTYWASTKQTAIV
                     LLGLLDLMKARRETGAPSEVEVVVNGASIGTHRFAAGSLAAPDPVAFSVPAQAGRNTV
                     RLVTRGEGAIYWSVQAAYYDTGAARERTGSHALALQREYFSLAPVTVKGRVVYRATAL
                     RGPAQPGDLLLVRLTAAGARDWRYLVLEDPIPAGTEPIQRDDLYQLERGRTDWWGSRR
                     EFRDSRAVFFQERFDQGRYEYSYLLKVIAPGTFRASPARISAMYVPDGFASSAAAEVV
                     VRSGAPPAEPAASKGGRQ"
     gene            complement(5949..6614)
                     /locus_tag="PLT35_04715"
     CDS             complement(5949..6614)
                     /locus_tag="PLT35_04715"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HOG28561.1"
                     /translation="MDGRRALIAAGILLLLRALPGSAQVRLADEADREAFRAWFTFLA
                     DAEFYRPTPDVADCAGLVRHAAREALRAHTPEWHRLAALPALAPFPDVRDRPAGGPGG
                     LPLFRVAPGRYAEFADARTIVTLNARLIGRDPRALQPGDLVYFRQASGASPDHLMVFV
                     GRSRLERAAADWIVYHTGPADGGAGEVRKTRLADLVRHPAPRWRPVPHNPAFVGIFRL
                     DWL"
     gene            complement(6593..>7419)
                     /locus_tag="PLT35_04720"
     CDS             complement(6593..>7419)
                     /locus_tag="PLT35_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOG28562.1"
                     /translation="GGLGFRRDEQYTARGSKEPTLVIDRMAASRPAHPDAEARFTARS
                     VAFLRALERHNRRDWFRARKARYDEVVRGPMLALVQRFAADLPAFAPDLVADPRVSLY
                     RPYRDTRFSADKAPIKTHIGALFPHRLLPRHEGAGFYVEVAPHRVWYGGGMYMPSTTA
                     LQQVREHVAAHHRRLARIVSAPAFRRTYGVLEGNRLQRVPRGFPADHPAADFLKHRQF
                     LAGCERPAEFAASPAFYRTVLAAFKALAPLTAFLNEPLLAALRSGELGRPWTDAAR"
BASE COUNT         1046 a         2647 c         2740 g          986 t
ORIGIN      
        1 tgtggttccc gaagccgatc gcgcgcgcga gccggacatc agggccgccg ccaggcgtgc
       61 gctcaaccgg gtggccggtg cgctcggcgt cgagccgccc gcggagctgg acctggtgtt
      121 ccatcccacc gtcgagtcgt tcgcgcgggc cacgggccag ccgtggtgga ccgccgcgcg
      181 cacgagcgga tctcgcatcg atctgctgcc gcctcgcgtc ctggaagggc gccgcacgct
      241 cgaatcgaca ctgcggcacg agttcgtgca cctgctgacc gagcccgtcc tcgcgggccg
      301 cgcgctgtgg attcgtgaag ggctggcggt ggtcgtggcg ggggagctgc cgtcggccgg
      361 cgccgacgcg cgggggcgcg gcggcgccag ggccgccgcg tgcccgtcgg acgaggacct
      421 gcgcgccccg cgggatcctg acgcgtggcg ccgcgcgtac gaagccgccg ggcgctgcgt
      481 gtcagaggcg ctcgccggcg gccgccgctg gcaggatctc cgctgagggc ttcgcgcgct
      541 cgcgcggcgc cctgcgcagg atgaacgccc atcccgcatt cgcgatgatg aagatcgcga
      601 gcagcttggc cgccgcaaat gccggctgca cccacgtagg cggcagccac gcgggcctcc
      661 agtaggccgc atgccacggc agccagaacc ccgccatgag cgctgcgctg acggcaatca
      721 gccgcggtcc cgacaagccg gaccgcaccc agcccgtgcc cagcccccgc aagccccgca
      781 cggcgagcgc cctcagcagc gcggcggcca gcgacacgcc gatgccgtat cgcacgaacg
      841 ccacgatcca gccggctgcc gagtgcaggc cggtcgtgcg ggtccatccc gtgcgggcta
      901 tcacccaggc gtcgatgcgt cccgcgttct gctcgagcca cccggtggca tcagccgtga
      961 cgagccagaa gcagccgaag acggccagcg gaaacacgat gagccaggcg cgccgtgcgc
     1021 cgaggcgggc ggcctcgcgc atcggcatga ccgcgttgaa gccgagcgca gcggccatct
     1081 ccacccatcc cagcagcagg accgccgcga tggcgacgag ggcggaggcg aggagcatcc
     1141 acgcgttgga ttccggaact tgcagcagca gccaatagag gcccccgaca gcggcgtggc
     1201 ccgccgagag ccacgccagc acgatgcgcg tccgggtcgt cactgccggc ctcccttcga
     1261 cgccgccggc tcggccggcg gagcgcccga gcggacaacc acctcggcgg cggcgctcga
     1321 cgcgaagccg tcgggtacgt acatcgccga gatccgggcg ggcgaggccc ggaacgttcc
     1381 cggcgcgatc accttcaaca ggtacgagta ctcgtaccgg ccctggtcga agcgctcctg
     1441 gaagaacacg gcccggctgt cacggaactc gcgccggctg ccccaccagt cggtgcgccc
     1501 ccgctcgagc tggtacaggt cgtcgcgctg gatcggctcg gtgcccgcgg ggatcggatc
     1561 ctcgagcacg aggtagcgcc agtcgcgggc gccggcggcc gtgaggcgca cgagcagcag
     1621 gtcgccgggc tgcgccgggc cccggagcgc cgtcgcccgg tacacgacgc ggcctttgac
     1681 ggtcaccggc gcgaggctga agtactcgcg ctgcagcgcc agggcgtgcg atcccgtgcg
     1741 ctcgcgcgcc gcgccggtgt cgtagtacgc ggcttgcacg gaccagtaga tcgcgccctc
     1801 cccgcgtgtc acgagcctga ccgtgttccg gccggcctgg gccggcaccg agaatgccac
     1861 cgggtccggc gccgcgaggc tgccggcggc gaaccggtgc gtcccgatgc tggcgccgtt
     1921 gacgacgacc tccacctcgg acggcgcgcc ggtctcccgg cgcgctttca tgaggtcgag
     1981 caggccgagg aggacgatgg cggtctgctt cgtgctggcc cagtacgtgc cgaaggtgcg
     2041 gttcagcagg agccatctga cagcaggctc gatcagcgga tgcttcgggt ccctggcggc
     2101 gagggcctgc gcggccagcg cggtggcttc gacgctcgtg tcgacgaaat cgaagagcag
     2161 cgggtcgttg gcgctcgtcc accacgccag gtcacccttc cgctccgccc gcgatgccac
     2221 gtcgcgcgcc agcgcgtcgc cgcgcgcgtc cttcgcggcg tcgagcgcga gcagcagcag
     2281 gctccgcccg tacgcgctca tgcggtcgcg cgcggaccac aagtcgtcga gggcggccgt
     2341 ccggctccac cccttgccct cgcctcgcgc aggcgcctcg aacccggctg ccgccgcggc
     2401 gagcagcaca taggtttcgt aggccttcaa gtcggggagt gcggcggggt actcggcata
     2461 gagcgcgccg agtgcccgca cgccgcggcg gatcggccac tcttccacct tgtagccggc
     2521 cgcgcgggct tccagtaggc cggtgatcgc gtaggccgtc atgaacggat ggttctggtc
     2581 cgtcttccac cacccccatc cgccgtcttc gtgctgggag tcgagcaggc gcgccaggcc
     2641 ctctgtgacc tggcggtcga gcgacttcag cccttcggtg agcggcagct gcaggtcggt
     2701 cagcgtccgc cgggccacca ggttcggcag gaggctggag accgtctgct cggtgcaccc
     2761 gtacgggtag ctggtcagga agtcgagggc ggcgaggatc ggccctccga gcgacggcgc
     2821 gacctggacc cggatggcgc gctcggacgg attcgaggac gccggcacgt ccacctcaac
     2881 cacctgctcg ccggcgcctc gcatggaccc ggctcgcccc tcttcgcgct gcaggccgaa
     2941 ggggagcacc gggaaggaga gttggatcgc gtctgcgtcg gcggcggcag ccgccgtgcc
     3001 ggagacgact gccgcgccgg cgcgcggggc gtcgatcgtc caggccagcg cgcgctcggc
     3061 gccggccggc acctccacgg tctgcggcgt ggcccggctc ggatcaccgc cggcgaaggt
     3121 gaggccctca acctctccgg tgaccttcac cgcctgggta tccggcaggt agttgtggac
     3181 gatgaccggc atgtcgatcc ggtccgcctc ggtgaggaag cggggcgtca ccacgcgcag
     3241 gatgaggtcc ttcgtggtcg tcgtcctagc caggccggcg ccgacgagcg tgtcagcggt
     3301 gacggcgcgt gccgtcaggc gccacgtcgt cagcgcgtcg ggatacggca cgcggaccgt
     3361 cgcccgcccc tgcgcgtcgg tcgtgacgtc ggcggcccag aagatcgcgt cggggaagtc
     3421 cttgcgcacc tgcgggcgcg gcggtccctc ctgcttgaag tcggcgaggg tgtacggcct
     3481 tcgccgctgc gccagctgca gctcgtgcga tccggcgtat ccgacgaacg agtagtcccg
     3541 ggagtaatcg gtggacacgc ggctgtaact cagccggtgg aagaaacgga gcgcgtccgg
     3601 cgtgtcgtct ggcttcacgc cgtacacggc ttcgtcgatc acgccgacgc tcaactgggc
     3661 cctcacgggc gcgccgcttg cgtcggtgac ggtcagcgtg aagaccccgg cctcgcgcgg
     3721 cttcgccgtg gcggattccg cggcgaccgc gacgttcagt cggcgcgaga cggcgggcac
     3781 tttcagccgc ttctccgcgc ggtacaggcg atcgtccttc acgtacgcga tgttcacgta
     3841 cgtgtccccg agatccgcgt ccgacactgg cacctcgatg gcgtgctcgc cgccggtcct
     3901 gacgacgcgg tggaaggaga tctgccgggc ctctttcgtg acgagcacgg gcgccgagac
     3961 ggcgccgccc cgcacgacca gcctggcggt gtcgccgggg gcgtacgagg cgcggtccgc
     4021 gacgagttcc agataggtgt cgccgccgcc gaactcgtcg gccgaaccgg tcacccagac
     4081 gccgctcgtg tcctcgacgc gccggccggc gctctcggcc gacacgcgga agcggtacga
     4141 gccaggctcc tgcgggagcg ccgcggacca cgaggcccgg ccctgctcat ccgtcgtcgc
     4201 ggtcccggag gcgatgattg tcacctgagg ctcgttccac cgtccctgcg ggtacttgag
     4261 gcgctcgaga gccaggtgga gcgccacgcc cgcctgcagc acgccttcgt aatcgacggc
     4321 gcggatgctc acctgggccg tcgagccggg cttctggacg taccgatcga cgtccgagac
     4381 gacgaggaag cggccccagg tggcgtgcac gaaggcatgc cccgcgactt cccggttgct
     4441 ggcgtcggtg acccgcgcct cgatgcgagc ggtgtagtcg cgcccgtcgt cgtcaaccgg
     4501 cagcggaatc gacacggccg ccgtgccggc gtcgtcgagg cggacagtga ccgactcgga
     4561 ctcctcgccg ccgccccacc agccgcccgt gtcgtcgccg tcgtcgctcc accgcagcgg
     4621 cgagtagtac ggctggcggt gcacgacgta ggtcaccgag cctccagcca cgggctggcc
     4681 gaagtagtag cgcgccttga agaccgcctc gaccgcgccg ccctgcacga cgaatcgatc
     4741 ggcggcggtc accgtgacct cgaactccgg cttccggtac tcctggacct cgaagctgcc
     4801 gctcgcgcgt tcgtcgccgc ttgccgccgt gacgacgtac ccgccgagcg cggcgcccgc
     4861 gggcagcgcg aacgagctgc tgaccgagcc gaacgcgtcc accgggcgcc tctcgcgcag
     4921 gacgaccttg tcgttggagt ccgtgacgga cagctcgacg tcgctgccgc cgaagggctc
     4981 gagcccggcg gcggtgcgcc accgcaagac ccccttcatg cgcacggtgt gcccggggcg
     5041 gtagaccggt ttgtcggtgt agaggtagcc gacgagttcg cgggccggcg cctgcagcgc
     5101 ccagctgccg ggatcggtga ccgccacctg gccctcgcac gtcgcgagcg cgatgacggt
     5161 gtccggcttc ggctgcgcaa tcgccacgag ggcggtgccg tcggcgcccg ttgtcgcggc
     5221 ttcgagggga cggccgtcca cgatggtccg caggccgcac ccggcctgcg gctcgccggt
     5281 cccccggtcc acggcgtaga cgagcgcctg gcccggggcc gtcttcgtga cgagcgcgac
     5341 gtcggagaca atgacgatcg tgtaggcctt gagcggcggg cccacggcct cgacgacgta
     5401 gacgcccggt gcgctgacgt cgagggggat tcgcctcgat tcgggctcgc gcaccggcgg
     5461 caggagctcg cgccacgagg cgaccaggcg cgaggggttc aacagcggga cctgcgcgaa
     5521 gctgttgtag tccagcggca cccgccggcg cacgtctcgc gtggcccgcg cctcgcgccg
     5581 cttggctcgg tattcgcggc tgacctgccg gcgaacaaac gatcgcagcg ctttccgctg
     5641 tccggccttc cacgcggcga tgcgctcgat cgggctccgc tcctgcggaa caatcggggc
     5701 ttcgctgccg agctgatggg gatcgggcag cgacgcgaag aaggcgaaag gttcggcgac
     5761 gcggtagacc ctgaagtcga ggtgatcgac ctcgcggtac gtcagcgaga acgacggccg
     5821 gtcggtcgtc gtgaacacct ggctgctcca gagatggaag gccggacccg cgtcgccggc
     5881 gggctgggcg gacgccggag cgacggcgag gaacactaaa cccgcggcgc acggaagcag
     5941 atgtcgtgtc atagccagtc caggcggaag atgcccacga acgcggggtt gtggggcacc
     6001 gggcgccagc gaggcgccgg gtggcggacg aggtcggcga gccgggtttt ccgcacttcg
     6061 ccggccccgc catcggcggg accggtgtgg tagacgatcc agtcggccgc ggcgcgctcg
     6121 aggcgcgagc ggccgacgaa caccatcagg tgatcgggcg acgcccccga ggcctgcctg
     6181 aagtagacca ggtcgccggg ctggagcgcg cgcgggtcgc ggccgatcaa gcgcgcgttg
     6241 agcgtcacga tcgtcctcgc gtccgcgaac tcggcgtagc ggccgggagc gacgcggaac
     6301 agcgggaggc cgccggggcc gccagccggc cgatcgcgca cgtcgggaaa cggcgcgagc
     6361 gcgggcaggg cggccagccg atgccactcg ggcgtgtggg cgcgcagcgc ctcgcgcgcg
     6421 gcgtggcgca cgaggcccgc gcaatcggcc acgtcgggcg tgggtcggta gaactcggcg
     6481 tcggccagga aggtgaacca ggcccgaaag gcctcgcggt ccgcctcgtc ggcgaggcgg
     6541 acctgcgccg agcccgggag agcccggaga agaagcagga tcccggccgc gatcagcgcg
     6601 cggcgtccgt ccatggccgc cccagctcgc cggaccgcag agccgcaagg agcggctcgt
     6661 tcagaaacgc cgtgagcggc gccagcgcct tgaacgccgc gagcaccgtc cgatagaagg
     6721 ccgggctggc ggcgaactcg gccgggcgct cgcagccggc aaggaactgg cggtgcttca
     6781 ggaagtcggc cgccgggtga tcggccggga agccccgggg cacgcgctgc agccggttgc
     6841 cctccagcac gccgtacgtc cggcggaacg ccggcgcgga cacgatgcgc gcgaggcgcc
     6901 ggtggtgggc ggcgacgtgc tctcgcacct gctgcagggc ggtcgtcgac ggcatgtaca
     6961 tcccgccgcc gtaccagacg cgatgcggcg cgacctcgac atagaagccc gcgccttcgt
     7021 gcctgggcag gaggcggtgc gggaacagcg cgccgatgtg cgtcttgatc ggggccttgt
     7081 cggcggagaa ccgcgtgtcg cggtacgggc ggtacaggga cacgcgggga tcggccacaa
     7141 gatccggggc gaaggcgggc aggtcggccg cgaaccgctg cacgagtgcc agcatcggcc
     7201 cgcgcaccac ttcgtcgtag cgcgccttcc gcgcgcgaaa ccagtcgcgg cggttgtggc
     7261 gctcgagggc gcgcagaaag gcgacggacc gcgcggtgaa gcgcgcttcg gcgtcggggt
     7321 gcgcgggccg ggacgcggcc atccgatcga tcacgagcgt tggctccttc gatccgcgtg
     7381 cagtgtactg ctcgtcccgc cggaatccaa gcccgccgg
//
LOCUS       DAOSIF010000094         7401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9181_length_7401_cov_3.652464, whole genome shotgun sequence.
ACCESSION   DAOSIF010000094 DAOSIF010000000
VERSION     DAOSIF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9181_length_7401_cov_3.652464"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..2857)
                     /locus_tag="PLT35_04725"
     CDS             complement(77..2857)
                     /locus_tag="PLT35_04725"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HOG28563.1"
                     /translation="MSNPSRIAFVVVLAFMAASTGLARQAPAARQNPLLVTALRTEYA
                     ENPIGIDVAAPRLSWQIRASARGVIQASYQIRVASTERALAAPKGLVWDSGIVSSEES
                     TYRPYAGQPLQSGRRYYWQVRVWDRSGQASDWSQAAFWEMGLLTPADWKASWIEPGLQ
                     EDVTKSGPAPMLRREFVITGPIERARVYVTSHGMYEMELNGRRVGDAVLTPGWTSYHN
                     RLQYQTYDVTSQLQKGRNAIGVTFGNGWYRGNLAWADGRNHYGERLALLAQLVLTYRG
                     GKQEVVGTDAGWKAATGPILMSEIYHGETYDARLEKSGWSSPGFADADWSPVKVADYP
                     KTNLVAPAGPPVRRIEELKPVQTIRTPAGETVVDMGQNMVGWVRLKVQGPAGATVKLR
                     HAEVLDKQGNFYTENLRGAKQTVEYTLKGAGVETFEPHFTFQGFRYVAVEGFPGELAP
                     DSLTGVVVHSDMARTGAFETSKPLVNQLQHNIVWGQKGNFVDVPTDCPQRDERLGWTG
                     DAQVFARTAAFNMDVAGFFAKWLRDVAADQLPSGSVPHVIPDILSRPDRPSAGSAAWA
                     DAAVIIPWTMYLSYGDKRLLETQYPSMMRWVEYIRRRAGDDYIWSEDFAYGDWLAYAS
                     TASDYPGATTGKDLIATAFFAHSTDLVSRAARVIGRNDDAATYETLLGRIKNAFVREF
                     VTATGRVGENTQTAYAVALQFDLLPEAMRAGAAARLAREINQRKHLTTGFVGTPYLLH
                     ALARYGYLDEAYLLLNRETYPSWLYPVKQGATTIWERWDGLKPDGTFQDKGMNSFNHY
                     AYGAVGEWMYRVMAGLEIDPAAPAYKHALIQPRPGGGFTSVKASHDTPYGVIGSAWTL
                     KDGQFELVVDVPPNTSARVWLPGATLASATPVEVWRSGPFAGVLGGGFVLRFGGEKAP
                     TGRVSGPASGAR"
     gene            complement(2946..3740)
                     /locus_tag="PLT35_04730"
     CDS             complement(2946..3740)
                     /locus_tag="PLT35_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOG28564.1"
                     /translation="MKRREFLRSATLAGGAVMAGGAARAGATEGDGREFYELRVYETN
                     LGTRKRALDDYLERAFIPALNRAGSSPVGAFNVIAGASSLALYLLVPFPTLDALVSAP
                     ARLAADPEYVKAAEPCLSAPIDDPAYVRYESSLLRAFAEAPRVRVPAETAAKQPRLFE
                     LRTYESHSERAALRKIEMFNEGGEIALFDRLGLRSVFFGQTLMGRRQPNLVYMTVHRD
                     QAARDRSWAEFSASPDWKRLSSDPAFANTVSAQHIVFLRPAAYSQI"
     gene            complement(3649..5046)
                     /locus_tag="PLT35_04735"
     CDS             complement(3649..5046)
                     /locus_tag="PLT35_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28565.1"
                     /translation="MSRILPFLAAAAVLSPQNPPGALYDEAKVPSYVLPDPLITTAGE
                     RVRDSKTWIGTRRPEILELYRREVFGRVPSGAPRVSFQVESTDRQALDGVAVRKIVAL
                     TFGGGAAARAARMLVYLPAGARESVPVFLSLSFGPIQTVHADPGIPLGCQWSLDADSQ
                     EAVCRPAAESSRGSLAGRWPVEAILGRGYGLAAIYYGEIEPDVVGGLRHGIRALALKE
                     GQREPAPDEWGAIGAWAWSLSRALDYLETDRDVDAARVAVIGHSRLGKTALWAGAEDT
                     RFSLVISNDSGEGGAALSRRTFGERTRDLNTRFPHWFCGNFKQYNDRESEMPFDAHFL
                     LALAAPRGLYVASAAEDLWADPRGEFLAASAVEPVYALFGKSGLGTSGPAALDTPIGR
                     TVRYHVRSGKHEITAYDWDRYLDFADAMWKQAPRPGGGLGGGPHEETRVPQVGDTGGR
                     GGDGGRRGPGGGHGG"
     gene            5380..6891
                     /locus_tag="PLT35_04740"
     CDS             5380..6891
                     /locus_tag="PLT35_04740"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOG28566.1"
                     /translation="MRLLDAGQRVGARPPDGSCADLPTRPLLMDHLGRSIVCARRNPD
                     HRFAVVLVELDRSGEPGTGRPSPGAEGLLKAVAARLEACLERLDPSETACRSHLVARL
                     RGDEFAVLLDRVRDAGDAQAVASRMLAEALAPFPMGDRQVYLAPSVGIALGGAAHARA
                     EDVLRDADMALCRAKLLGKARCEIFDPDLATAAESQAALESDIRSALERGQFVLHFQP
                     ILALASNRIAGFEALVRWEHPSLGAIPPAEFIPMAERTGLITALGSWVLREACLRLKA
                     WQERGLVSEDLWVSVNLASPHFSRPGLADQIAATLGEMGLGARRLMLELTESAAMENA
                     AAIRRTIADVRAMGARVGLDDFGTGQSSLACLHQFPSDFLKLDRTFVRDLAIREDART
                     IVGAVIALARQLGLCVIAEGIETREQAELARALGCDYGQGFYFSHPLDQAGAADLLRA
                     GFPPPAVTSAGGESAGEAAPPPLESGLLPFDLPSCWTGWARPPAFAPTTLPPG"
     gene            complement(7096..>7401)
                     /locus_tag="PLT35_04745"
     CDS             complement(7096..>7401)
                     /locus_tag="PLT35_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28567.1"
                     /translation="LCMLLYGLAFGTVVTSGLALTLAVVVDPSNAGRSMALASATTFL
                     AQSIASGAGAVVLDPLNRLRPNAGYAGLVALIELLLAGGGYFLTRVDVPAPARRRPA"
BASE COUNT         1038 a         2685 c         2535 g         1143 t
ORIGIN      
        1 ctcttgtggg ccatgccccc cctctccggt gagcggcccg ttctcatttg gaaatcgacc
       61 acggccccca ttttttctac cgcgccccgg atgcggggcc gcttacccgg cccgtgggcg
      121 ccttctcacc cccgaaccgc aggacgaacc cgccgccgag cacgcccgcg aaggggccgc
      181 ttcgccagac ctcgactggc gtcgcgctcg ccagcgtcgc gcccggcagc cagaccctgg
      241 cgctcgtgtt gggcggcacg tcgaccacca gctcgaactg cccgtccttc agcgtccagg
      301 ccgacccgat cacgccgtag ggcgtgtcgt gcgacgcctt gacgctggtg aaccctccgc
      361 ccgggcgcgg ctggatgagc gcatgcttgt aggcgggcgc ggccggatcg atctcgaggc
      421 cggccatcac gcggtacatc cactcgccca ccgccccgta ggcgtagtgg ttgaacgagt
      481 tcatgccctt gtcctggaac gtcccgtcag gcttcaggcc gtcccagcgc tcccagatcg
      541 tcgtcgcgcc ctgcttcacc ggatagagcc acgacgggta cgtctcgcga ttcagcagca
      601 gatacgcctc gtctaggtac ccgtagcggg cgagggcgtg gaggaggtac ggggtgccga
      661 cgaaccccgt ggtcaggtgc ttgcgctggt tgatctcgcg cgccagccgg gcggcagcgc
      721 cggcccgcat ggcctcgggc agcaggtcga actgcagcgc gaccgcgtac gccgtctgcg
      781 tgttctcgcc gacccggccc gtggccgtga cgaactcgcg gacgaaggcg ttcttgatcc
      841 gcccgagcag cgtctcgtac gtcgcggcat cgtcgttccg gccgatcacc ctggccgccc
      901 gggacaccag gtccgtcgag tgcgcgaaga aggccgtcgc gatcaagtcc ttccccgtcg
      961 tcgcgcccgg gtagtccgac gcggtgctgg cgtaggcgag ccagtcgccg taggcgaagt
     1021 cctcgctcca gatgtagtcg tcgcccgcgc gcctccggat gtactcgacc cacctcatca
     1081 tgctcgggta ctgcgtctcg agcagccgct tgtcgccgta gctcaggtac atcgtccacg
     1141 ggatgatgac cgccgcgtcg gcccacgcgg ccgacccggc cgacgggcgg tccggccggc
     1201 tcaggatgtc cggaatcacg tgcggcacgc tgcccgacgg gagctgatcg gcggccacgt
     1261 cgcgcagcca cttcgcgaag aacccggcca cgtccatgtt gaacgccgcc gtccgcgcga
     1321 agacctgggc gtctcccgtc cagcccagcc gctcgtcccg ctgcgggcag tcggtgggca
     1381 cgtcgacgaa gttgcccttc tggccccaga cgatgttgtg ctgcagctgg ttcaccagcg
     1441 gcttcgacgt ctcgaacgcg cccgtgcgcg ccatgtccga gtgcacgacg acgcccgtca
     1501 ggctgtcggg cgcgagttcg ccgggaaagc cctcgaccgc cacgtagcgg aacccctgga
     1561 acgtgaagtg cggctcgaac gtctcaacgc ctgctccctt gagtgtgtac tcgaccgtct
     1621 gcttcgcgcc gcggaggttc tcggtgtaga agttgccctg cttgtcgagc acctcggcgt
     1681 ggcggagctt caccgtcgcg ccggcgggcc cctggacctt cagccggacc cagccgacca
     1741 tgttctggcc catgtccaca acggtttcgc cggccggcgt ccggatcgtc tggaccggct
     1801 tgagctcttc gatccggcgc accggcggac ccgccggggc cacgaggttc gtcttcgggt
     1861 agtcggcgac cttcacgggc gaccagtcgg cgtcagcgaa gccgggtgac gaccagcccg
     1921 acttctcgag gcgggcgtcg tacgtttctc cgtggtagat ctccgacatc aggatcggcc
     1981 ccgttgcggc cttccagccg gcatcggttc caacgacttc ctgcttcccc ccccgatagg
     2041 tcaggacgag ttgggccagc agggcgagcc gttcgccata gtggtttcgc ccgtcggccc
     2101 aggcgagatt gcctcggtac cagccattcc cgaaggtcac gccgatggcg ttcctcccct
     2161 tctgcagctg cgacgtcaca tcgtaggtct ggtactgcag gcgattgtgg tagctcgtcc
     2221 acccgggcgt cagcacggcg tctccgacgc ggcggccgtt cagttccatc tcgtacatgc
     2281 catggctcgt cacgtagacg cgcgcgcgct cgatcggccc ggtgatcacg aactcgcgcc
     2341 tcagcatcgg ggccgggccc gacttggtga cgtcctcttg cagcccgggc tcgatccaac
     2401 tggccttcca gtcagcggga gtgagcaggc ccatctccca gaaggcggcc tggctccagt
     2461 ctgaggcctg gccgcttcgg tcccagacgc gcacctgcca gtagtagcgc cggcccgact
     2521 gaagcggctg gcctgcatac ggccggtagg tggattcctc cgacgacacg atgcccgagt
     2581 cccagacgag acccttgggg gccgccagcg cgcgttccgt cgaggccacg cggatctggt
     2641 acgacgcctg gatgacgccc ctcgcgctgg cgcggatctg ccagctgagt ctcggcgcgg
     2701 cgacgtcgat gccgatgggg ttctcggcgt actcggtgcg aagggcggtc acgagcaggg
     2761 ggttctgcct ggctgcaggc gcctgcctgg ccagccctgt gctcgcggcc atgaacgcga
     2821 ggacgaccac gaatgcgata cgcgacgggt tgctcatgcg acactccagt gcacggtggt
     2881 tccggccggc tgccttgcgc ttcgcgggcc ggccgccagg gcctgcctcg gcgcagcggg
     2941 gcccgtcaaa tctgcgagta cgcggcggga cgcaggaaga cgatgtgctg cgccgagacc
     3001 gtgttggcga acgccgggtc ggaactcagc cgcttccagt ccggcgaggc gctgaactcg
     3061 gcccacgacc tgtcccgcgc cgcctggtcc ctgtgtacgg tcatgtacac gaggttcggc
     3121 tgccggcgcc ccatcagcgt ctgcccgaag aagaccgatc gcaggcccag ccgatcgaac
     3181 agggcgatct cgcctccttc gttgaacatc tcgatcttcc tgagcgctgc ccgctcgctg
     3241 tggctctcgt acgtgcgcag ctcgaaaagg cggggctgct tcgcggcggt ctcggccgga
     3301 acgcgcacgc gcggcgcctc ggcgaacgcc ctgagcaacg agctctcgta gcgcacgtac
     3361 gcggggtcgt cgatgggcgc ggagaggcac ggctcggccg ccttcacgta ctccgggtcc
     3421 gccgcgagcc tggccggcgc ggacaccagg gcgtcgagcg tcgggaatgg cacgaggagg
     3481 tagagggcga ggctgctcgc ccccgcgatg acgttgaacg cccccaccgg tgagctgccc
     3541 gcccggttga gcgccggaat gaaggcgcgc tcgagatagt cgtcgagcgc cctcttccgc
     3601 gtgccgaggt tcgtctcgta gacgcgcagt tcgtagaact cccggccgtc accctccgtg
     3661 gcccccgccc gggccgcgcc gcccgccatc accgccccgc ccgccagtgt cgccgacctg
     3721 aggaactcgc gtctcttcat ggggcccccc tcccaatccc ccaccgggtc tcggcgcctg
     3781 cttccacatc gcgtccgcga agtccaggta ccggtcccag tcgtaggcgg taatctcgtg
     3841 cttgccggag cgcacgtggt agcgcaccgt ccgcccgatt ggcgtatcga gtgccgcagg
     3901 cccggaggtg ccgagccccg acttgccgaa cagcgcgtac accggctcca cagccgacgc
     3961 cgcgaggaac tctccccgcg gatcggccca aagatcctcg gcggcgctgg ccacgtagag
     4021 gccgcgaggg gctgccaggg ccagcaggaa gtgcgcgtcg aacggcattt cggactcgcg
     4081 gtcgttgtac tgcttgaagt tgccgcagaa ccagtgcggg aacctcgtgt tgaggtcgcg
     4141 cgtgcgctcg ccgaacgtgc ggcggctgag cgccgcaccc ccttcgcccg agtcgttgga
     4201 gatcacgagc gagaatcgcg tgtcttcggc cccggcccaa agggccgtct tgcccagtct
     4261 cgagtggccg atgacggcga cgcgcgccgc atccacatcg cggtcagtct cgaggtagtc
     4321 gagcgccctg ctgagcgacc aggcccacgc gccaatcgcg ccccactcgt ccggcgccgg
     4381 ctcccgctgc ccttccttga gggccagcgc gcggatgccg tgcctcagcc ctccgacgac
     4441 gtcgggctcg atctcgccgt agtagatcgc cgcaagcccg tatccccggc cgaggatggc
     4501 ctcgacaggc cagcggccgg ccagcgagcc ccgcgacgac tcggcggcgg gccggcagac
     4561 ggcctcctgc gagtccgcgt cgagcgacca ctggcagccg agcgggatgc ctgggtcggc
     4621 gtgaaccgtc tggatcgggc cgaacgacag gctgaggaac accggaaccg attcccgggc
     4681 gccggccggc aggtagacca gcatccgcgc cgcgcgcgcg gcagcgccgc cgccgaacgt
     4741 caacgccacg attttccgga ccgcgacgcc gtccaacgcc tgccgatcgg tcgactccac
     4801 ctggaacgag acgcgcggcg ctccggacgg cacgcggccg aacacctctc tgcggtagag
     4861 ttcgagaatc tccggccggc gcgttccgat ccatgtcttc gagtcccgca ctcgttcccc
     4921 ggccgtcgtg atcagcgggt ctggcaggac gtaggatggg accttcgcct cgtcatagag
     4981 ggcacccggc gggttctgtg gagacagcac ggcggccgct gcgagaaacg gcaggattcg
     5041 gctcatgctc ggctccgggc tggcgggcgg ctcgccccgg gggcggttcc agaaccctac
     5101 gacgcggcgg caacgctgtc aaggcgcatc gccgactcgg ctaatgccgg cggcccctcc
     5161 gaccgaaaag acggcgggga cggggctgtc tccccccggc ggccgggctc gcgagagcca
     5221 gccgggcggg acggcgttcc cggggtcacc ccgcaaccgg cctcatcttt tcgcaacatg
     5281 aaccacggcg agtgggctgc gcacggcggc gacgagggat tcgggccctg gggccggaac
     5341 gtcgacccgc atcttccccc gcgggccggc ggcccccccg tgcgcctgct cgacgcgggc
     5401 cagcgcgtcg gcgccaggcc gccggacggc tcctgtgcgg acctgccgac acgtccgctc
     5461 ctcatggatc acctagggcg atccatcgtc tgcgcgcggc gcaacccgga ccatcgcttc
     5521 gcagtcgtgc tggtcgaact cgaccgatcc ggggagccgg gcacaggccg cccttcgccc
     5581 ggcgccgagg gcctgctaaa ggccgtggcc gcacggctgg aggcgtgcct cgagcggttg
     5641 gatccgagcg agacggcctg ccggtcccat cttgtcgcca ggctcagagg cgacgaattc
     5701 gccgtgctgc tcgatcgcgt gcgggacgcg ggcgacgccc aggcagtggc aagccgcatg
     5761 ctggccgagg cgctcgcgcc attccccatg ggcgatcggc aggtgtatct cgctccgagc
     5821 gtagggatag ccctgggggg cgccgcccac gcgcgggcgg aggacgtcct gcgggacgcc
     5881 gacatggcgc tctgccgagc gaaactgctg ggcaaggccc gatgcgagat cttcgatccg
     5941 gacctcgcca cggcagccga gtcccaggcc gcgctcgagt ccgatatccg aagcgccctg
     6001 gagcgcggcc agttcgtcct gcacttccag cccatcctgg cgttggcgtc gaaccgcatc
     6061 gccggtttcg aggccctcgt gcgctgggag cacccgtcgc tcggcgcgat ccctccggcg
     6121 gagttcatcc cgatggccga gcggacgggg ctgatcacgg cgcttggcag ctgggtgctg
     6181 cgcgaggcgt gtctccggct caaggcctgg caggagcggg gcctggtttc cgaggacctg
     6241 tgggtctcgg tcaacctcgc gagcccgcac ttcagccgcc cgggtttggc cgaccagatt
     6301 gccgcgacgc tcggtgagat gggactcggc gcccgccgcc tgatgctgga gttgaccgag
     6361 agcgccgcga tggagaacgc cgccgcgatc cgacgcacga tcgccgacgt ccgggccatg
     6421 ggcgcccgcg tcgggctgga cgatttcgga acgggacagt cctcgctggc ctgcctccat
     6481 cagttcccct ccgatttcct gaagctcgat cggacgttcg tccgcgacct tgcgatccgc
     6541 gaagatgccc ggaccatcgt gggcgccgtc atcgcgctcg cgcgtcagct cggcctttgc
     6601 gtgatcgccg agggcatcga gacgcgcgag caggcggaac tcgcccgcgc gcttggctgc
     6661 gactacggac aaggcttcta cttctcgcat ccgctggacc aggcgggcgc ggccgatctg
     6721 ctgcgggcgg ggttcccccc gccggcggtg acgagtgcgg gcggcgagtc cgccggcgag
     6781 gctgcgccgc cgccgctcga gagcggcctg ctgcccttcg accttccaag ctgctggacg
     6841 ggctgggcca ggccgcccgc attcgcgccg acaacgctcc cgcccggcta gctccacggc
     6901 cgacgcacct tccccgcgag accgcccgag ccagcggctg gcgaactggc cgggatctcc
     6961 gccccggagg caccgccgtg cccggtgcgc tcgccgcctc cgccctgctc tgccggctcg
     7021 gccggacggg tccggcgatc tcgccgccat cgatcgcggc ggaagccaac cgcgcggggc
     7081 gcgggcggat ttagttcacg cggggcgtcg gcgcgcgggc gccggcacgt cgaccctcgt
     7141 caggaagtat ccgccgccgg ccaggagcag ttcgatgagt gcgaccaggc cggcgtaacc
     7201 tgcgttcggg cggagccggt tgagtgggtc cagcactacc gcgccggcgc cggatgcgat
     7261 cgattgagcc aggaacgtcg tcgccgacgc gagggccatg ctccttcccg cgttggaggg
     7321 gtcgaccacg accgccagcg tcagcgccag gcccgatgtg acgacggtgc cgaaggcgag
     7381 gccgtagagg agcatgcaca g
//
LOCUS       DAOSIF010000095         7388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9206_length_7388_cov_3.749489, whole genome shotgun sequence.
ACCESSION   DAOSIF010000095 DAOSIF010000000
VERSION     DAOSIF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9206_length_7388_cov_3.749489"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="clpX"
                     /locus_tag="PLT35_04750"
     CDS             <1..768
                     /gene="clpX"
                     /locus_tag="PLT35_04750"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOG28568.1"
                     /translation="IILKLLQAAGGDIEKCQQGIIYIDEIDKISRKDENPSITRDVSG
                     EGVQQALLKILEATVANVPPQGGRKHPHQEFYQVDTTNILFICGGAFVGLDKVVARRI
                     GKRAMGFRADAGAAASRDAGALLERLEPEDLIKYGLIPEFVGRLPVVGTLHELDKAAL
                     VQILTEPKNAITKQYQRLFEYENVKLRFTDDALEAIAEGAIKRKIGARGLRTIIEDLM
                     LDLMYSLPGQKKVKECVITREVVEHRAQPLTLMEKAG"
     gene            768..3161
                     /gene="lon"
                     /locus_tag="PLT35_04755"
     CDS             768..3161
                     /gene="lon"
                     /locus_tag="PLT35_04755"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG28569.1"
                     /translation="MELYETLPIVPLRDLVVFPHMMVPFVIGRPASIRALEHAQEKDN
                     RVFLAAQHDAQVDDPQPQDIYSMGCVANVVQSLKLPDGNIKLLVEGAERGRAIEWKED
                     RGFFRVVVKVLPRHRDAAGDVEGAMSRVVSLFEQYVKLSNSLQYDAMIAAVRVDDPGR
                     LSDTIAAHLLVGVEEKQNLLEMVSPLERLNRIASILETEVDKLQVDRRIQTRVKKQME
                     KAQKEYYLNEKMRAIQKELGRKDERGNEIDDLRKKIEKARMPKDVEEKALQELKRLEA
                     MPPMSAEATVSRNYLDWLVGVPWHKRTKESRDLKRAEAILNADHYGLEKVKERILEFL
                     AVRALVKKPKATILTFAGPPGVGKTSLAKSIARAMNRRFVRLSLGGVRDEAEVRGHRR
                     TYIGAFPGQIIQMMKKAGTQNPLFLLDEVDKMSMDFRGDPSAALLEVLDPEQNHAFLD
                     HYLDVEYDLSNVMFICTANVLHTVPQALRDRMEVLNLAGYTEQEKLEIAKRFLVPKAL
                     EGTGLLPANVSFTDDAIQTIIQRYTREAGVRGLEREIQSICRKVARRVVTEGRAHAES
                     VSSERIAAFLGVPKFRPTEAEAKNEVGLATGLAWTEVGGEILVTETTLMSGRGRLTLT
                     GKLGDVMQESAQAALSYVRSKAAEYGIDKDFPRRHDVHIHVPEGAIPKDGPSAGITMA
                     TALVSVLTGTPVRRDVAMTGEITLRGKVLPIGGVKEKVLAAHRAGVTNIVLPKDNEKD
                     LADIPRTVLDVLNVYLVETMDEVLRVALATPLPGRPLADAAIEPVRVDRPSDERVTH"
     gene            3326..3955
                     /gene="yihA"
                     /locus_tag="PLT35_04760"
     CDS             3326..3955
                     /gene="yihA"
                     /locus_tag="PLT35_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOG28570.1"
                     /translation="MARAGIVAAEFVTSVAAAAGLPQSPLAEVALVGRSNVGKSSLLN
                     ALAGRTIARTSAAPGKTRLANFYRLEPRRGTACHVVDLPGYGYARGAGDGRPFEPLAR
                     DYFFGGRAPDRLRGAIAAAVLLADARHPGLDADLGAAAWLTAHGCPYQVAAAKADKLS
                     RGERQRAAESFARAFGRDVLMLSARTGEGLESLWTWIAQQVEQWTQRRP"
     gene            3934..5214
                     /gene="rho"
                     /locus_tag="PLT35_04765"
     CDS             3934..5214
                     /gene="rho"
                     /locus_tag="PLT35_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG28571.1"
                     /translation="MDATQTVDVAQLDLASLKTMSVAGLTAIAKQLDLPGATGLRKQD
                     LIFQILRARSERSGLMFSEGVLETLPDGYGFLRGPDYNYLPGPDDVYVSPSQIRRFDL
                     HTGDTVSGQIRPPREGERYFALVKVDAVNFEPPERKRERVYFENLTPLYPQEKFNLEF
                     AADELCTRVIDMMVPIGKGQRGLIVAPPRTGKTMLLQALANSVMANHPDVFLIVLLID
                     ERPEEVTDMQRSVRGEVISSTFDEPPQRHVQIAEMVIEKAKRLVETGRDVLILLDSIT
                     RLARAYNTVVPASGRILSGGVDSNALQKPKRFFGAARKIEGAGSLTIVATALIDTGSR
                     MDDVIFEEFKGTGNLEILLDRKLLDRRVYPSIDLQKSGTRKEELLLDKETLNKVWVLR
                     KVLDPLSTVDAMELLLGKLSKTKSNAEFLAAMHR"
     gene            5627..6592
                     /locus_tag="PLT35_04770"
     CDS             5627..6592
                     /locus_tag="PLT35_04770"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG28572.1"
                     /translation="MKYRPFGRVGTPVAEIGFGTWGMGGWSGSDDTASRAALRRGLEL
                     GCNFLDTARAYGDGHSERLIRDVLSGWDGPAPFIATKVPPKNGRWPARPDYLLDDVFP
                     ADHVRRATEESLENLGIDRLDLQQFHVWTDAWAADDRWQRAVADLKAEGLVKAFGISI
                     NRWEPANAIRALQTGLIDAVQVVYNIFDQAPEDELFPACRELGVAVIARVPFDEGSLT
                     GTMTASDTWPASDWRSLYFTPDKLRATLARVERLQADLPDGMALPELALRFILHHPAV
                     TTVIPGMRKVGHVERNLAASDGRAFPPALLDAMRRHRWDRDEVIP"
     gene            6856..7131
                     /gene="rpmE"
                     /locus_tag="PLT35_04775"
     CDS             6856..7131
                     /gene="rpmE"
                     /locus_tag="PLT35_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOG28573.1"
                     /translation="MKEGIHPKYFDVVARCACGATWKTRSTKPELHLEICSSCHPFYT
                     GRQKIMDTEGRIERFTKKYGAQTSEARKKAAATRKASKKAAGAAARS"
BASE COUNT         1268 a         2561 c         2568 g          991 t
ORIGIN      
        1 atcatcctga agctgctgca ggcggccggc ggcgacatcg agaagtgcca gcagggcatc
       61 atctacatcg acgagatcga caagatctcg cgcaaggacg agaacccgtc gatcacgcgc
      121 gacgtgtcgg gcgaaggcgt gcagcaggcg ctgctcaaga tcctcgaggc caccgtggcc
      181 aacgtcccgc cgcagggggg gcggaagcac ccgcaccagg agttctacca ggtcgacacg
      241 accaacatcc tgttcatctg cggcggcgcc ttcgtcgggc tcgacaaggt ggtggcccgg
      301 cggatcggca agcgggccat ggggttccgc gccgacgccg gcgcggcggc cagccgcgac
      361 gccggggcgc tgctcgagcg gctggagccg gaggacctca tcaagtacgg cctgatcccg
      421 gagttcgtcg gccggctgcc cgtcgtcggg acgctccacg agctggacaa ggcggcgctc
      481 gtgcagatcc tgaccgagcc gaagaacgcg atcacgaagc agtaccagcg gctcttcgag
      541 tacgagaacg tcaagctgcg cttcacggac gacgccctcg aggccatcgc cgagggcgcg
      601 atcaagcgga agatcggcgc gcgcggcctc cggacgatca tcgaggacct gatgctggac
      661 ctcatgtact cgctgcccgg ccagaagaag gtgaaggagt gcgtgatcac gcgcgaggtc
      721 gtcgagcacc gggcgcagcc gctcaccctc atggagaagg cgggctagtg gaactgtacg
      781 agacgctccc catcgtgccg ctgcgggacc tggtggtgtt cccgcacatg atggtgccct
      841 tcgtcatcgg gcgcccggcg tcgatccgcg ccctcgagca cgcgcaggag aaggacaacc
      901 gcgtgttcct ggcggcgcag cacgacgccc aggtggacga cccgcagccc caggacatct
      961 actcgatggg gtgcgtggcg aacgtcgtcc agagcctgaa gctgcccgac ggcaacatca
     1021 agctgctcgt cgagggcgcc gagcgcgggc gggcgatcga gtggaaggag gaccgcgggt
     1081 tcttccgggt cgtcgtcaag gtgctgcccc gccaccgcga cgccgcgggg gacgtcgagg
     1141 gcgcgatgag ccgcgtcgtg tcgctgttcg agcagtacgt gaagctgtcg aactccctgc
     1201 agtacgacgc gatgatcgcc gcggtgcggg tggacgaccc cggccgcctc tcggacacga
     1261 tcgccgccca cctgctcgtg ggcgtcgagg agaagcagaa cctgctcgag atggtgtcgc
     1321 ccctcgagcg cctgaaccgc atcgcctcca tcctcgagac cgaggtcgac aagctccagg
     1381 tcgaccgccg gatccagacg cgcgtgaaga agcagatgga gaaggcccag aaggagtact
     1441 acctcaacga gaagatgcgg gccatccaga aggagctggg ccgcaaggac gagcgcggaa
     1501 acgagatcga cgacctcagg aagaagatcg agaaggcccg gatgccgaag gacgtcgagg
     1561 agaaggccct gcaggagctg aagcgcctcg aggcgatgcc cccgatgtcc gccgaggcca
     1621 ccgtgtcgcg caactacctc gactggctcg tcggcgtgcc gtggcacaag cgcacgaagg
     1681 agagccgcga cctcaagcgc gccgaggcca tcctgaacgc cgaccactac ggcctcgaga
     1741 aggtcaagga gcggatcctc gagttcctcg ccgtccgcgc cctcgtcaag aagccgaagg
     1801 ccacgatcct gaccttcgcg ggcccccctg gcgtcggcaa gacctccctc gcgaagtcca
     1861 tcgcccgcgc catgaaccgc cggttcgtcc gcctctccct cggcggcgtg cgcgacgagg
     1921 cggaggttcg cggccaccgc cggacgtaca tcggcgcgtt ccccggccag atcatccaga
     1981 tgatgaagaa ggccggcacg cagaacccgc tcttcctgct ggacgaagtc gacaagatgt
     2041 cgatggactt ccgcggcgat ccgtcggcgg cgctcctcga ggtcctcgac ccggagcaga
     2101 accacgcctt cctcgaccac tacctggacg tcgagtacga cctctcgaac gtcatgttca
     2161 tctgcacggc caacgtcctg cacaccgtgc cgcaggcgct gcgcgaccgc atggaggtgc
     2221 tgaacctggc gggctacacc gagcaggaga agctggagat cgccaagcgg ttcctcgtgc
     2281 cgaaggccct cgagggcacg ggcctgctgc cggcgaacgt gtcgttcacc gacgacgcga
     2341 tccagacgat catccagcgc tacacgcgcg aggccggcgt ccgcggcctc gagcgcgaga
     2401 tccaatccat ctgccggaag gtcgcgcgcc gcgtcgtcac cgagggccgc gctcatgccg
     2461 agagcgtctc gtcggagcgg atcgccgcgt tcctcggcgt cccgaagttc cggcccaccg
     2521 aagccgaggc caagaacgag gtcggcctgg ccaccggcct ggcctggacc gaggtcggcg
     2581 gcgaaatcct cgtcaccgaa accacgctca tgtccggccg cggccgcctc acgctcacgg
     2641 gcaagctcgg cgacgtcatg caggagtcgg cgcaggcggc cctgagctac gtgcggtcca
     2701 aggccgccga gtacggcatc gacaaggact tcccccgccg ccacgacgtt cacatccacg
     2761 tgcccgaggg cgccatcccg aaggacggcc cgtcggcggg catcacgatg gcgaccgccc
     2821 tcgtgtcggt gctgaccggc acgccggtcc gccgcgacgt cgccatgacg ggcgagatca
     2881 cgctgcgcgg caaggtgctg ccgatcgggg gcgtgaagga gaaggtcctc gccgcccacc
     2941 gcgccggcgt cacgaacatc gtcctgccga aggacaacga gaaggacctc gcggacatcc
     3001 cgcgcaccgt gctggatgtc ctcaacgtct acctggtcga aaccatggac gaggtgctgc
     3061 gggtggcgct ggcgacgccg ctgccgggcc gccccctggc ggacgcggcc atcgagccgg
     3121 tgcgcgtcga ccgcccgagc gacgagcgcg tcacgcacta gcctgagacc cggcctgccg
     3181 ccgcagcggg ccggcaggcc gttcagcgaa gacgggggcg cagggctcgg gaggcggcgc
     3241 cggacgtggc ccccggctcg gggcgcccgc ctggactgcg acgcggcggg ggccgtcccg
     3301 gctgccctgc cgcgcaccgg acaccatggc gagagccgga atcgtcgcgg cggagttcgt
     3361 caccagcgtt gcggccgcgg cgggactgcc gcagtcgccg cttgccgagg tggcgctcgt
     3421 cggccgctcc aacgtcggca agtcgagcct gctcaacgcc ctcgccgggc gcacgatcgc
     3481 ccgcacgagc gcggcgcccg gcaagacgcg cctggcgaac ttctaccggc tcgagccgcg
     3541 ccgtggcacg gcctgccacg tggtggacct gccgggttac ggctacgcga ggggcgccgg
     3601 ggacggccgg ccgttcgagc cgctcgcgcg ggactacttc ttcggcggcc gggcgccgga
     3661 tcgcctcagg ggggccatcg cggccgccgt gctgctggcc gacgcgaggc accccggcct
     3721 ggacgccgat ctcggggcag cggcctggct gacggcgcac ggctgcccgt accaggtggc
     3781 ggcggccaag gcggacaagc tgtcccgcgg ggagcggcag cgcgccgcgg aatccttcgc
     3841 gcgcgcgttc gggagagacg tgctgatgct gtcggcgcgg accggggaag ggctcgagtc
     3901 tctgtggacg tggatcgcac aacaggtgga acagtggacg caacgcagac cgtagacgtc
     3961 gcgcagctcg acctcgcgtc gctcaagacg atgagcgtgg cgggcctgac cgccatcgcg
     4021 aagcagctcg accttcccgg cgccacgggg ctccggaagc aggacctcat ctttcagatc
     4081 ctcagggccc gcagcgagcg cagcggcctg atgttctccg agggcgtcct cgagaccctg
     4141 ccggacggct acgggttcct gcggggcccc gactacaact acctcccggg cccggacgac
     4201 gtctacgtgt cgccgtcgca gatccggcga ttcgacctgc acaccggcga caccgtgtcg
     4261 ggccagatcc gcccgccccg cgagggcgag cggtacttcg ccctcgtcaa ggtggacgcg
     4321 gtgaacttcg agccgccgga gcgaaagcgc gagcgcgtct acttcgagaa cctcacgccg
     4381 ctctacccgc aggagaagtt caacctcgag ttcgccgccg acgagctgtg cacccgcgtg
     4441 atcgacatga tggtgcccat cggaaagggc cagcgcggcc tcatcgtggc gccgccgcgc
     4501 acgggcaaga ccatgctgct gcaggcgctg gcgaacagcg tcatggcgaa ccaccccgac
     4561 gtcttcctca tcgtcctgct catcgacgag cggcccgagg aggtcaccga catgcagcgg
     4621 tccgtgcgcg gcgaggtgat ctcgtccacc ttcgacgagc cgccccagcg ccacgtgcag
     4681 atcgccgaga tggtcatcga aaaggccaag cggctggtgg aaaccgggcg cgacgtgctg
     4741 attctgctcg actcgatcac gcggctcgcg cgggcctaca acacggtggt gccggcgtcg
     4801 gggcgcatcc tgtcgggcgg cgtggacagc aacgcgctcc agaaacccaa acgcttcttc
     4861 ggcgccgccc gcaagatcga aggggcgggc tcgctgacca tcgtcgccac cgccctcatc
     4921 gacaccggct cccgcatgga cgacgtcatc ttcgaggagt tcaagggcac cggcaacctc
     4981 gagatcctgc tcgatcggaa gctgttggac cgccgcgtct atccgtcgat cgacttgcag
     5041 aagagcggca cccgcaaaga ggagctcctg ctcgacaagg agacgctcaa caaggtctgg
     5101 gtcctccgga aggtgctcga tccgctgtcc accgtcgacg ccatggagct gctcctgggg
     5161 aagctgtcga aaaccaagag caacgccgag ttcctggcgg caatgcatcg ctagagacaa
     5221 aagggaatgc cggggcacca tggcggcgct cggccgccac gtcgtcgcct gccgacaggc
     5281 cgcctggcga gagcgggatt caggattcgg gattcgaaat ccggcattcc ggatttctcg
     5341 ggtctcttga gaagcgcgct gcggcccagc ctgcgcccgg tcgcgtaacc cattgattgg
     5401 acggaggtta gagaacgact ccgtgcgcca gaacaccgct tcgtcggcat ggggttcgca
     5461 tgcggaatct cacggggagg gaggctcgcc cgagtaccgc gtgctcgtct ggtcggcaac
     5521 gaccgaggga gacgcatgag gggcagcgtg cacagacgcg ctgcccctcg gcgtgtaccg
     5581 gccgcgcagg agcccggtgg gcacgtcaca gggcaggact gacgaaatga agtacagacc
     5641 attcggacga gtgggaaccc cggtcgctga gattggtttt ggcacctggg gcatgggcgg
     5701 ctggagcggg tccgacgaca cggcctccag ggcggcgctt cgcagggggc tggaactcgg
     5761 ctgcaacttc ctcgacacgg cgcgcgcgta cggcgacgga cacagcgagc gcctcatccg
     5821 cgacgtcctc tccggctggg acggcccggc gcccttcatc gccacgaaag tcccgccgaa
     5881 gaacgggcga tggcccgcaa ggccggacta cctcctcgac gacgtgttcc cggcggacca
     5941 cgtgcgccgg gctaccgagg agagcctgga gaacctgggg atcgatcgcc tcgacctgca
     6001 gcagttccac gtgtggaccg acgcctgggc ggccgacgac cgctggcagc gcgccgtcgc
     6061 cgatctcaaa gcagagggcc tagtgaaggc cttcgggatc agcatcaacc gctgggagcc
     6121 ggccaacgcg atccgggccc tccagacggg gctcatcgat gccgtccagg ttgtctacaa
     6181 catcttcgac caggcgccgg aagacgagtt gttccccgcg tgccgggaac tgggtgtcgc
     6241 cgtcatcgcc cgcgtgccgt ttgacgaagg cagcctgacg gggacgatga ccgcgagcga
     6301 cacgtggccg gcgagcgact ggcgcagcct gtatttcacg cccgacaagc tccgggcgac
     6361 gctggcgcgc gtcgagcgcc tccaggccga ccttccggac ggcatggcat tgccggagct
     6421 ggcgttgcgc ttcatcctgc accaccctgc cgtcacgacg gtcatccccg ggatgcggaa
     6481 ggtcggccat gtcgagagga acctcgccgc ttccgacggc cgggccttcc cgccagccct
     6541 gctcgacgcc atgcggcggc accgctggga ccgggacgag gtcattccgt aggcgatagg
     6601 gcctgggcgc tcggcgctcg tcaggcaggg acaaggagca aagcctgcct gccgaagctg
     6661 gccggcaggc cgttcagcga aggcgagggg ccgggcagcc ggggcgagga cggcctcgcg
     6721 gccgcggcac cggcggcgtg ccggcgcgag cgcgccatcg gcgcgcgccc gccgcacgct
     6781 gtgccccgct tggttcgtgg cggcggggcg tacctgctat acttggaaga tcgcaggagg
     6841 cgtgaaggag cggtcatgaa ggaaggcatc cacccgaagt acttcgacgt tgtcgcgcgc
     6901 tgcgcgtgcg gggcgacgtg gaagacccgt tcgacgaagc ccgagcttca cctcgagatc
     6961 tgctcgagct gccacccgtt ctacaccggc cggcagaaga ttatggacac cgaaggccgc
     7021 atcgagcggt tcacgaagaa gtacggcgcg cagacctctg aggcccgcaa gaaggcggcc
     7081 gccaccagga aagcctcgaa gaaggccgcc ggcgcggcgg cccggagctg aagccggcca
     7141 tccccgcagg ggcgccccgc cagcgggcgc cccgcgtgct cccccacccg tttgacgccc
     7201 ggcccgcccc gcggtaacgt ccccgcggag gctccgccat gccgcatgcg tcgcatcggt
     7261 cccgtcaatc gctcgcgagc gcggccgccg cagccgtcct ggcggcagcc ggcctcgccg
     7321 cggccgcctc gaaccgggcg gctgcaccgg ccggtgagag cttgtgaggc ggcaatacag
     7381 tgcaaacg
//
LOCUS       DAOSIF010000096         7384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9212_length_7384_cov_4.402783, whole genome shotgun sequence.
ACCESSION   DAOSIF010000096 DAOSIF010000000
VERSION     DAOSIF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9212_length_7384_cov_4.402783"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..398)
                     /locus_tag="PLT35_04780"
     tRNA            complement(309..398)
                     /locus_tag="PLT35_04780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Ser,seq:gga)
     gene            complement(564..2546)
                     /gene="glgB"
                     /locus_tag="PLT35_04785"
     CDS             complement(564..2546)
                     /gene="glgB"
                     /locus_tag="PLT35_04785"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOG28574.1"
                     /translation="MRKTILSDLDLHLFNEGNHYRAYEKLGSHAATFDGESGLHFAVW
                     APNADRVSVVGDFNRWDGRVNVCEPLHSSGIWTTFVPGLAWGEKYKYEVRAKNGALVL
                     KADPYARRFEVPPRSATIAWDASGYQWNDQAWIAARAASGHQLERPVSIYEVHLGSWR
                     RGLANEALSYRDLADQLVPYVTEMGYTHVELLPVMEHPFTGSWGYQVTGFFAPTSRFG
                     PPEDFKRFIDACHQAGIGVILDWVPGHFPKDGYGLIRFDGTALYEHEDTRLGEHKEWG
                     TLVFNYGRHEVRNFLLSNALFWLDEYHADGLRVDAVASMLYLDYSRKDGQWLPNRFGG
                     RENLDAIDFLRQLNIVVHEQFPGALMIAEESTAWPAVSRPVYTGGLGFTFKWNMGWMH
                     DMLRYMSKDPVHRKWQHNDITFSMLYAYTENFVLPFSHDEVVHGKRAMVSKMPGDTWQ
                     RFANLRALYGYMFGHPGKKLNFMGGEFGQTREWDHDHSLDWHLAVYPEHKGLQQLVRD
                     LNRVYREEPSLHELDVRPEGFSWIDCNDHESSIVSFVRRSKDPADFTLFILNFTPVVR
                     HGYRLGVPEAGWYREVINTDAAAYGGSNVGNQGRVPADAIPTHGYEHSVSLTVPPLAC
                     LMLKPDRDRAARAPAEPQAGAPAGAPGEPPAGPREG"
     gene            complement(2543..3457)
                     /locus_tag="PLT35_04790"
     CDS             complement(2543..3457)
                     /locus_tag="PLT35_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOG28575.1"
                     /translation="MGRRCRYLHLDVFTREKFGGNQLAVFTDARGLTAPIMQAMTREM
                     SFSECTFILPAERPDTDIRMRIFTPGREMPMAGHPTVGSAFALALDGVIPAGRRRWTF
                     GLNIGPTPVDLDWDGGRPAFAWMTQQRPVFGNAVDGVEEAARAIGAEAGAIRSTGLPV
                     QEVSCGVPFVLFPVASRADVDAAAPDLDAFRRLCRRAGLDDHAMFVFSLEPGGDGATV
                     YSRMFAPGLGVAEDPATGAASGPLGCYLVKYGAVPPERAGSILSVQGVKMGRPSRIHV
                     AIGLDGAAIATVQVGGEAVLAGEGELFL"
     gene            3571..6327
                     /locus_tag="PLT35_04795"
     CDS             3571..6327
                     /locus_tag="PLT35_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG28576.1"
                     /translation="MCVHRFLAAPLAGALLAVMAAASAMAQPAAGIDIPYKRFVLKNG
                     LTLIVHEDRKAPIVAVNVWYHVGSKNEQPGRTGFAHLFEHLMFNGSEHFDDDYFKVLD
                     RLGATDLNGTTNEDRTNYFQNVPTPALDTALWMESDRMGHLLGAITQAKLDEQRGVVQ
                     NEKRQGENEPYGKVDLAIAEATYPKGHPYSWTVIGSMEDLAAASLDDVKKWFTSYYGP
                     SNAVLVIAGDIDAETARQKVERYFGDIPPGPPVTKHQAWIAKRTGEQRQVMEDRVPMA
                     RIYMVWNVPQWGSADADLLGLAANVLATGKTSRLYKRLVYDDQIATDVSASLDSREIA
                     SQFYVMATVRPGMAPAKVEQALREELAALLKDGPTPAELERVKTQRRAAFVRGVERIG
                     GFGGKSDVLAQGEVYAGNPEQHAITQRRIAEATPAAVRDASSRWLSDGVYVLEVRPFP
                     QYATTPSTVDRSAVPTPGAPPAVRFPAFERATLANGLKLVVAPRQSVPQVNLTLLVDS
                     GFAADQSALPGTASLALDMMDEGTASRSALQIAEDVAGLGATLSLGATLDTAAVRMSA
                     LKENLDASLAIFGDVVLNPSFPADELERLRRRRLAQIQQEGAQPIGIALRVLPGLIYG
                     PGHAYGLPLTGSGTIESVGRITRDTLVKFHGDYFKPSNATLIVVGATTMAEIKPRIEK
                     LFAAWKPGPVPARNVRTVAAAKAQAVYVIDRPGAEQSLILAGHVAPPKSNPDEIAIEA
                     MNAVLGGQFVSRINMNIREDKHWSYGAQTLFWDARGQRPFIVYAPVQTDKTKEAIQEI
                     QKELRGILGQIPVSAEELAAAKNALVLTLPGQWETMSAVGATLQQLVTFGLPDDYFDT
                     YGAKVGALGAADLVRAAKVSVHPDSVVWVVVGDLKKIEAPLKALGLGDIRLLDADGKV
                     KPGS"
     gene            6427..7029
                     /locus_tag="PLT35_04800"
     CDS             6427..7029
                     /locus_tag="PLT35_04800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG28577.1"
                     /translation="MRRLLFCGLLVPAVVLAGTISLEAKLKPGIYAHFTTPLGKFTVR
                     LFDDKAPKTVANFVGLAEGTKEWTDPKTGSKVKRPFYEGLIFHRVIDGFMIQGGDPLG
                     VGTGGPGYKFEDEFHKDLRHSKEGILSMANAGPNTNGSQFFITLGPTPHLNDHHSVFG
                     EVVEGMDVVRKIGKVPKAPGDRPVTPVTMSVTIERVPAGK"
     gene            7106..7267
                     /locus_tag="PLT35_04805"
     CDS             7106..7267
                     /locus_tag="PLT35_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28578.1"
                     /translation="MRRIAAAMILALPGVLAALPAAADPLPAVSDRVVQYHIRAALDT
                     ASGKVLLPE"
BASE COUNT         1150 a         2585 c         2567 g         1082 t
ORIGIN      
        1 gagggattgg tgcaggggtg cgtccggcgt gcggcgcaga aggcgggcct gtacaacaac
       61 gggtcgcaca ggctgcgcca ctcgccctgt tcacatctcg cgatgcgggg cgcaccggct
      121 cgagcgattc aggagctggc gcgacatcag gatccgataa cgacgcagcg gcgcatgtac
      181 ctcagtccgg cggcgatcga gagcgcgatc cggctcctcg atcggccgga ttccctacag
      241 agccgtggag acactctgga gacgggcccg ggcgaaatgg tgaagtcgtt tgggtggaac
      301 aggttagctg gcggagagag agggattcga accctcggta gggtttcccc tacacacgct
      361 ttccaagcgt gcaccttcag cctctcggtc atctctccgc gacggagcag gcgtgcgccc
      421 cgatcttagc acgcggaccg cgcggagccc gcggcgccgg gcgggcgcgc ggcgccgtcc
      481 ccggcgcctc cgttcaggga gaccgcgacg tgcgccgccg gcgcgcggct gggcgaggcc
      541 cgcgcagccc cccgcgcgac cggctaccct tctctcggcc cggccggcgg ctcccctggc
      601 gcgccggcgg gagctccggc ctgcggctca gccggcgccc tggcggcacg gtctcgatcc
      661 ggcttcagca tcaggcacgc gagcggcggc acggtcaggc tgaccgagtg ctcgtagccg
      721 tgcgtcggga tggcgtcagc cggcacgcgg ccctggttgc cgacgttcga gccgccgtaa
      781 gcggcggcgt cggtgttgat cacctcgcgg taccagcccg cctctggaac gccgagccgg
      841 tacccgtgcc ggacgacggg cgtgaagttc aggatgaaga gcgtgaaatc ggccgggtcc
      901 ttcgatcgcc ggacgaagga cacgatcgag ctctcgtggt cgttgcagtc gatccacgag
      961 aagccctccg ggcggacgtc cagctcgtgc agcgacggct cctcgcgata gacccggttg
     1021 agatcgcgga cgagctgctg gaggcccttg tgctcggggt agaccgcgag gtgccagtcc
     1081 aggctgtggt cgtggtccca ctcccgcgtc tggccgaact cgccgcccat gaagttcagc
     1141 ttcttgccgg gatgcccgaa catgtagccg tagagcgcgc gcagattcgc gaaacgctgc
     1201 cacgtgtcgc cgggcatctt cgacaccatc gcccgcttcc cgtggacgac ctcgtcgtgc
     1261 gagaacggga gcacgaagtt ctccgtgtag gcgtacagca tggagaacgt gatgtcgttg
     1321 tgctgccact tccggtgcac ggggtccttc gacatgtagc gcagcatgtc gtgcatccag
     1381 cccatgttcc acttgaaggt gaacccgagg cccccggtgt acacgggccg gctgacggcg
     1441 ggccacgcgg tggactcctc ggcgatcatc agcgccccgg ggaactgctc gtgcacgacg
     1501 atgttcagct ggcggaggaa gtcgatggcg tcgaggttct ctcggcctcc gaaccggttc
     1561 ggcagccact gcccgtcctt gcgcgagtag tccaggtaga gcatcgaggc cacggcgtcc
     1621 acgcgcaggc cgtcggcgtg gtactcgtcc agccagaaca gcgcgttcga caggaggaag
     1681 ttgcgcacct cgtggcggcc gtagttgaag accagcgtgc cccactcctt gtgctcgccg
     1741 aggcgcgtgt cctcgtgctc gtagagcgcc gtgccgtcga accggatgag gccgtacccg
     1801 tccttcggga agtgcccggg cacccagtcg aggatcacgc cgatgcccgc ctggtggcac
     1861 gcgtcgatga agcgcttgaa gtcctccggc gggccgaacc ggctcgtcgg ggcgaagaag
     1921 ccggtcacct ggtaccccca cgacccggtg aagggatgct ccatcaccgg caggagctcc
     1981 acgtgggtgt agcccatctc ggtgacgtac ggcacgagct ggtccgccag atcccggtag
     2041 gagagcgcct cgttggcgag cccgcgccgc cacgagccga ggtgcacctc gtagatcgac
     2101 accggccgct cgagctggtg gcccgacgcc gcgcgcgcgg cgatccacgc ctggtcgttc
     2161 cactggtagc ccgaggcgtc ccacgcgatc gtcgccgacc ggggcggcac ctcgaaccgc
     2221 cgcgcgtacg ggtccgcctt cagcacgagc gcgccgttct tcgcccgcac ctcgtacttg
     2281 tacttctcgc cccaggcgag gccgggaacg aacgtcgtcc agatgcccga cgaatgaagc
     2341 ggctcgcaca cgttgacgcg gccgtcccac cggttgaagt cgccgaccac gctgacgcgg
     2401 tcggcgttcg gggcccacac cgcgaagtgc aggcccgact cgccgtcgaa ggtcgcggcg
     2461 tgagatccga gcttctcgta ggcgcggtag tggttgccct cgttgaagag gtgcaggtcg
     2521 aggtcggaca ggatggtctt cctcatagga acagctcgcc ttctccggcc aacacggctt
     2581 ctccgcccac ctggacggtc gcaatggcgg ccccgtcgag gccaatcgcg acgtggatcc
     2641 ggctggggcg ccccatcttc acgccctgca cgctcaggat cgagccggcc cgctccggcg
     2701 gcaccgcgcc gtatttcacg aggtagcagc cgaggggccc gctggccgcg cccgtcgccg
     2761 ggtcctcggc cacgccgagc ccgggcgcga acatccggct gtagaccgtc gcgccgtccc
     2821 cgccgggctc gagactgaac acgaacatcg cgtgatcgtc cagcccggcc ctccggcaca
     2881 gcctccggaa ggcatcgaga tccggcgccg ccgcgtccac gtccgcgcgg gacgccaccg
     2941 gaaagagcac gaagggcacg ccgcacgaca cttcctggac gggcaggccg gtggaccgaa
     3001 tcgccccggc ctcggccccg atagcgcgcg cggcctcctc cacgccgtcc acggcgtttc
     3061 cgaagaccgg cctctgctgc gtcatccagg cgaaggccgg gcgcccgccg tcccagtcga
     3121 ggtccacggg ggtgggcccg atgttcagcc cgaacgtcca ccggcgccgg ccggccggga
     3181 tgacgccgtc aagagccagg gcgaacgcgg acccgacggt cgggtggccc gccatgggca
     3241 tctcccggcc gggcgtgaag atccgcatcc ggatgtcggt gtccggccgc tctgccggta
     3301 gaatgaaggt gcactccgag aagctcattt cgcgcgtcat ggcctgcatg atcggcgcgg
     3361 tgaggccccg ggcgtcggtg aagacggcga gctggttgcc cccgaacttc tcgcgcgtga
     3421 acacgtccaa gtggagatag cggcagcgtc tgcccatgat ggatccgacc gctccctgcg
     3481 cgtgtcccat gctatacgat cacccgttct ccaggcccga gacggccccg gccctgcgac
     3541 gccggcccgc cccttccacg gaggtcccac gtgtgcgttc atcggttcct cgccgcgccg
     3601 ctggccggcg cgctcctggc cgtcatggcc gccgctagcg ccatggccca gcccgctgcc
     3661 ggcatcgaca tcccctacaa gaggttcgtc ctcaagaacg gcctcaccct catcgtccac
     3721 gaggaccgca aggcccccat cgtcgccgtg aacgtctggt accacgtcgg ctcgaagaac
     3781 gagcagccgg ggcggacggg cttcgcccat ctgttcgagc acctgatgtt caacggcagc
     3841 gagcacttcg acgacgacta cttcaaggtg ctcgaccgcc tcggggccac cgatctcaac
     3901 ggcaccacga acgaggaccg gacgaactac ttccagaacg tgcccacgcc ggcgctcgac
     3961 acggccctct ggatggagtc ggaccgcatg ggccacctgc tcggcgccat cacgcaggcg
     4021 aagctggacg agcagcgcgg cgtcgtgcag aacgagaagc gccagggcga gaacgagccg
     4081 tacggcaagg tggacctggc cattgccgag gcgacctatc cgaagggcca tccctattcg
     4141 tggacggtca tcggctcgat ggaggatctc gccgccgcct cgctggacga cgtgaagaag
     4201 tggttcacct cgtactacgg cccgtcgaac gccgtgctcg tcatcgccgg cgacatcgac
     4261 gcggagaccg cgcggcagaa ggtcgagcgc tacttcggcg acatcccgcc ggggcctccg
     4321 gtgacgaagc accaggcgtg gatcgcaaag cgcacgggcg agcagcggca ggtcatggag
     4381 gaccgcgtcc cgatggcgcg gatctacatg gtgtggaacg tgccgcagtg gggctcggcc
     4441 gacgcggacc tgctcggcct ggcggcgaac gtgctggcca cgggcaagac gtcgcgcctc
     4501 tacaagcggc tggtctacga cgatcagatc gcgacggacg tctccgcgtc gctcgattcg
     4561 cgcgagatcg cgagccagtt ctacgtcatg gccacggtgc ggcccggcat ggcccccgcg
     4621 aaggtggagc aggccctgcg cgaggagctg gccgcgctgc tgaaggacgg gccgacgccg
     4681 gccgaactgg agcgcgtcaa gacgcagcgg cgggcggcgt tcgtcagggg cgtcgagcgc
     4741 atcggcgggt tcggcggcaa gtccgacgtc ctggcgcagg gcgaggtcta cgcggggaac
     4801 cctgagcagc atgcgatcac gcagcggcgc atcgccgagg cgacgccggc cgccgtgcgc
     4861 gacgcctcct cgcgctggct ctccgacggc gtgtacgtcc tcgaggtgcg gcccttcccg
     4921 cagtacgcca cgacgccgtc cacggtggac cggtccgccg tgccgacgcc cggcgcgccg
     4981 ccggcggtgc gcttcccggc gttcgagcgc gccacgctgg ccaacggcct gaagctcgtc
     5041 gtcgccccgc ggcagagcgt gccgcaggtc aacctcacgc tgctcgtgga ctccggcttc
     5101 gccgccgacc agtccgcgct gcccgggacg gccagcctcg ccctcgacat gatggacgag
     5161 ggcacggcga gccgctcggc gctccagatc gccgaggacg tggccggcct cggcgcgacg
     5221 ctgagcctcg gcgcgacgct cgacaccgcg gccgtgcgga tgtcggcgct caaggagaac
     5281 ctcgacgcct cgctggctat cttcggcgac gtcgtcctca acccgtcgtt ccccgccgac
     5341 gagctcgagc ggctccggcg gcggcgcctc gcccagatcc agcaggaggg cgcccagccc
     5401 atcgggatcg cgctacgggt gctgccgggc ctgatctacg gccccggcca cgcctacggc
     5461 ctgccgctga cgggctcggg cacgatcgag tcggtcggcc ggatcacccg ggacaccctg
     5521 gtgaagttcc acggcgacta cttcaagccc tccaacgcga cgctgatcgt cgtcggcgcg
     5581 acgaccatgg ccgagatcaa gccgcgcatc gagaagctgt tcgccgcgtg gaagccgggc
     5641 ccggtgccgg ctaggaacgt gcgcacggtg gcggcggcca aggcgcaggc cgtctacgtg
     5701 atcgaccgcc ccggcgccga gcagtcgctg atcctcgccg gccacgtggc cccgccgaag
     5761 tcgaacccgg acgagatcgc catcgaggcg atgaacgccg tgctcggcgg gcaattcgtg
     5821 tcgcggatca acatgaacat ccgcgaggac aagcactggt cgtacggcgc ccagacgctg
     5881 ttctgggacg cccgcggcca gcgccccttc atcgtctacg cgccggtgca gaccgacaag
     5941 acgaaggaag ccatccagga gatccagaag gagctgcggg ggatcctcgg ccagatcccg
     6001 gtgagcgccg aggagctcgc cgccgcgaag aacgcgctcg tgctgacgct gccgggccag
     6061 tgggagacga tgtcggccgt cggcgcgacg ctgcagcagt tggtgacgtt cggcttgccg
     6121 gacgattact tcgacacgta cggggcgaag gtcggcgccc tcggcgcggc cgacctcgtc
     6181 cgcgcggcga aggtctccgt gcatcccgac agcgtcgtct gggtcgtcgt cggcgatctg
     6241 aagaagatcg aggcgccgct caaggcgctc gggctgggcg acatccggct gctcgacgcc
     6301 gacgggaagg tgaagcccgg ttcgtgagtg cggcgcccgg acacggcggg ttctgcggta
     6361 cgatgcgttg actggccccg cccccggggc gccggcaggg cggggccgtt gcgaggaggc
     6421 aacccgatga gacgtctgct gttctgcggc ctgctggtgc cggccgtcgt cctggccggc
     6481 acaatcagcc tggaggccaa gttgaagcct ggaatctacg cgcacttcac caccccgctg
     6541 ggcaagttca cggtccggct gttcgacgac aaggcgccga agaccgtcgc caacttcgtc
     6601 ggactggccg agggcacgaa ggagtggacc gatcccaaga cgggcagcaa ggtgaaacgc
     6661 cccttctacg agggcctcat cttccaccgc gtcatcgacg gcttcatgat tcagggcggc
     6721 gatccgctcg gcgtcggcac cggcggccct ggctacaagt tcgaggacga gttccacaag
     6781 gacctgcgcc actcgaaaga gggcatcctc tcgatggcga acgccgggcc gaacacgaac
     6841 ggcagccagt tcttcatcac cctcggcccc acgccgcacc tcaacgatca ccattccgtg
     6901 ttcggcgagg tggtcgaggg catggacgtc gtgaggaaga tcggcaaggt gccgaaggcc
     6961 ccgggcgaca ggccggtgac gccggtcacg atgagcgtga cgatcgagcg ggttccggcg
     7021 ggcaagtagc caacatgggg gctgggcgcc gggcgctggt gtgacgcgcc tggcccctcg
     7081 cccctttcac cctgcggagt ccgccatgcg ccgcatcgcc gcagcgatga tcctggccct
     7141 ccctggcgtc cttgcggcgc tgcctgccgc cgccgatccg ctcccggcgg tgtccgatcg
     7201 ggtggttcag taccacatca gggccgcgct cgacacggcc agcggcaagg tgttgttgcc
     7261 ggagtagcac gcgggacgtg atcggcggcc ccgggcgggg cgctttggct actccgcccc
     7321 gaaggcttcc gagccgttga ggtgcaggcc gttgctgttg gcggcggtgg ggcgggggac
     7381 ggcg
//
LOCUS       DAOSIF010000097         7364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9247_length_7364_cov_5.014229, whole genome shotgun sequence.
ACCESSION   DAOSIF010000097 DAOSIF010000000
VERSION     DAOSIF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9247_length_7364_cov_5.014229"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PLT35_04810"
     CDS             <1..1187
                     /locus_tag="PLT35_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG28579.1"
                     /translation="LEEHFRQFRDDTSQVRVTILATLQCNLACRYCYQGDHAGANGPA
                     GKMSAETAADVADWIARQVDEVRPIQLVLTFFGGEPLLNAPAMTAIAEACWRHTRQRQ
                     VRQAVNIITNGLLLTPDVVRAMLPFGLTGVKVTLDGDCETHDRLRPARGGRGTFDRII
                     GNLRQVAPLVPITIGGNFDTATASRYPALLDFLSAQDFAPRIAKVAFKPIIEPAAPSG
                     AGPAAPLCNGACMSAVGPSAGCGVEPASGAAQGARAAADDTMSFLHEETRRRGLPAPD
                     GVHMGPCEIHRRHSHTIGPDGTLYPCAGFTGDRALSIGHIDGRRTDSQAAAAERVERR
                     QPWRRCGDCPFIPVCGGGCAVASHAELGDMDAPSCHRRSFESALASLAEQAARDASGG
                     IQ"
     gene            1468..2856
                     /locus_tag="PLT35_04815"
     CDS             1468..2856
                     /locus_tag="PLT35_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG28580.1"
                     /translation="MPQSRQVDPSHGIAFLAETIAFERLTANDAAGRTQPRLLEGWSV
                     SADGLRWRLRVRAGVAFQDGAPLTAADVARTFEAAMADEAARGASVCLPYVASVAASG
                     DYDVEVRLARRCAYLLDDLDRAVSRPAADGRTRIGTGAFSIVSSTPDEVVMEANRTHY
                     LEAPAIDRVVIRPYDALRTAFADMMRGRVDFLWEVAPDAAEFLGDQRSVTVRAFPGYY
                     AYAVTMNSARPALRAAAVRRALDAAVNREALVQQGLRGRGVPADGPVWPRYWARDRAA
                     PPPAADAAEAAARLRAAGPIAFTCLVPANFAILERMALLVQQQLAAFGVKMRLESAPP
                     GAFNRRLLSGDFDAAMLSVLGGPSATVYHRFWHSPGAARRWNYWNYRDARVDAALEAA
                     LEAPDDEAFRKAIGAFQGAWRDNPAAIFLAWNQTVQAVSRRFTLPPGSETRDAIHVLH
                     GWQPEPPAGRTP"
     gene            2853..4643
                     /locus_tag="PLT35_04820"
     CDS             2853..4643
                     /locus_tag="PLT35_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG28581.1"
                     /translation="MSTLRVRFAAMLGTAAVLPLLMYGVVSVYSLREGTRRTVNDGNL
                     NLARQVGEQLSRYVSTNLLILAGLADDIGIAELDPDERDRILKNFILRFPEFREVTAL
                     AADGAVIATSRIGASTLTLPQAGGVSLHGVRISPVVVDADFLPTMLVAAPLGGGAQAP
                     GWLVGELSIEELWRLVSRVRVGAAGHAMVVGPAGELLAHGNPDERPAIARGASLASDP
                     VVAALRQAAPGAPVVRDRTTEAGEVFLSAGVAIPSLGWLVIVEQPAAEALAASRRQER
                     DLTAAIALGLLVMVLAGLIWGRSLIAPIQELIRGTEAIAEGRLDERVRITSRSELGRL
                     GDAFNKMAGRLGDLQEDVRRQERHAVFGRVAAGLVHDLSHPFKNIQNNCRLIVKMHDD
                     PEYLELFRRTIDRECGQVKRVFEDLRNLARPMPMERFPLDLNRLAADVAESMRANAET
                     AGLSLELDLAPGPLLVEGDVFALSRVCRNLVMNAIEATNPRGTVTIATRAAAAAAVLR
                     VSDTGCGIAPARLETLFESFATTKRQGLGLGLAIAKKIVDQAGGTITASSVVGRGSAF
                     EVTLALAAPAASGGETRHPRGTAGMPEAGP"
     gene            complement(4692..4991)
                     /locus_tag="PLT35_04825"
     CDS             complement(4692..4991)
                     /locus_tag="PLT35_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG28582.1"
                     /translation="MAEARRMGKLELFAHFADRFEVKRAVAREFFDELAMLSEKELKR
                     SGEFVLPGMVKLVVQKRKARQGRNPATGEPIKIPAKTVVKARVVKQLKDAVLPRK"
     gene            5176..5523
                     /gene="queF"
                     /locus_tag="PLT35_04830"
     CDS             5176..5523
                     /gene="queF"
                     /locus_tag="PLT35_04830"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOG28583.1"
                     /translation="MSSAKIETFPNPRPGREYEIAISAPEFTSVCPMTGLPDFGEIRI
                     SYVPAERCIELKSLKLYLVEYRNRGIFYEAATNQILDDLVTACQPARMTVVGSFAPRG
                     GIRTSITATYESP"
     gene            5520..5987
                     /locus_tag="PLT35_04835"
     CDS             5520..5987
                     /locus_tag="PLT35_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOG28584.1"
                     /translation="MTASSGSGRRPASPRLADGLPGEGDGSSRPGGRSSPLGQAQAGA
                     AGDRQQSRPEPGSGRAGAGPPNDDGAVRLPIDGTLDLHAFRPRDVRSVVDDYVNEASA
                     AGLRAIRIAHGRGTGVLRGIVQAALDAHPLVEAFWDDTDAHLGATWARLAARR"
     gene            6005..>7364
                     /locus_tag="PLT35_04840"
     CDS             6005..>7364
                     /locus_tag="PLT35_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOG28585.1"
                     /translation="MARGKAAFTVTLILGFALLVPPGGAAAQEIPTPEAFFGFKMGDD
                     GRLADWPSIEKYFRAVAASSPRVELIEVGPSTEDRRIIAAVISSTENIRDLPALQLDN
                     RLLADPRRLESDREVRAILSGAKAVVAIGCSIHASEVGATQSASELLYELAAASDERT
                     AAMLRDIIVVLIPSLNPDGHAMVVDWQRKTAGTPFDGGPMPWPYHKYVGHDLNRDAFM
                     LNMAESRALARFFSREWHPQVFLSMHQMGSNGPRFFVPPNADPIDSNQDPLIWREAAL
                     LGSAMAMELESQGKSGVVSNAMFDYFWPGYEDSAPLGRNTVCLLTEAASARLASPIEV
                     AAKELRGGGRGLPEYAPQINFPNPWPGGTWRLRDIVDYNLAAMRGLLSAVARYRGEIL
                     ADFHAMGRRAIARGAAEPPFAFAIPPDQHDPAAAAALVNLLADGGVEVHQASEAFAVG
                     QTE"
BASE COUNT         1036 a         2684 c         2627 g         1017 t
ORIGIN      
        1 cgctcgagga gcacttcagg cagttccggg acgacacgtc ccaggtccgc gtcacgatcc
       61 tcgcgacgct ccagtgcaac ctggcgtgcc gctactgcta ccagggagac cacgccggcg
      121 cgaacgggcc ggccggaaag atgtcggcag agacggccgc cgacgtggcg gactggatcg
      181 cgcgccaggt ggacgaggtg cggcccatcc agctcgtgct cacgttcttc ggcggcgagc
      241 cgctgctgaa cgcgccggcg atgacggcga tcgccgaggc gtgctggcgg cacacccggc
      301 agcgccaggt gcggcaggcg gtcaacatca tcacaaacgg cctcctgctc acgccggacg
      361 tcgtccgggc catgctgccg ttcgggctga ccggcgtcaa ggtcacgctg gacggcgact
      421 gcgagaccca cgaccgcctg cgccccgcgc gcgggggccg cgggacgttc gaccgcatca
      481 tcggcaacct ccggcaagtc gcgccgctcg tgcccatcac gattggcggc aacttcgaca
      541 cggcgacggc gtcgcgctac cctgcgctgc tcgacttcct gagcgcccag gacttcgcgc
      601 cgcgcatcgc caaggtcgcc ttcaagccga tcatcgagcc ggctgcaccg tccggcgccg
      661 ggccggccgc gccgctatgc aacggcgcgt gcatgagcgc ggtggggccg tcggccgggt
      721 gcggggtgga gccggcgtcg ggcgccgcgc aaggggcccg cgcggccgcg gacgacacga
      781 tgtcgttcct gcacgaggaa acgcggcggc ggggcctgcc cgcgccggac ggcgtccaca
      841 tgggcccgtg cgagatccac cggcgccact cgcacaccat cgggccggac ggcacgctct
      901 atccgtgcgc ggggttcacc ggggatcgcg ccctctcaat cggccacatc gacggccgcc
      961 gcaccgacag ccaggctgcg gccgccgagc gcgtcgagcg ccggcagccg tggcgccgct
     1021 gcggggactg tcccttcatc ccggtgtgcg gcggcggctg tgccgtcgcg tcgcacgccg
     1081 agctcggcga catggatgcg ccgtcatgtc accgtcgaag cttcgaatcg gcgcttgcat
     1141 cgctggcgga gcaagccgcc cgcgacgcgt caggagggat acaatgaaag tcaaggttct
     1201 caagaaggcg cagaaggccc cgtcggcgga aatgtgcccg tggatgattt gcgtgccgcc
     1261 cgagagcggc aagtaaggga ctcccggacc accaggggac aggcagacag ccgcctcgcc
     1321 cgcgcggccc ccgcgcgcgc ccggtcccgt tggcggcgcc gccgagcggc gctcgccgcc
     1381 tccgccgccg gcgcgctgtc tgccgtcgcc tgcgccgcgc ccgcgcccgc tcctcggccc
     1441 acccaacccg tcacgctcac catcggcgtg ccgcagagcc gccaggtcga tcccagccac
     1501 ggcatcgcgt tcctcgccga gacgatcgcc ttcgagcggc tcacggccaa cgatgccgcc
     1561 ggccggaccc agccgcggct cctggaaggc tggtccgtgt cggccgacgg cctgaggtgg
     1621 cgcctgcgcg tgcgcgccgg cgtcgcgttc caggacggcg ctcccctgac ggctgccgac
     1681 gtcgctcgca cgttcgaggc cgcgatggcc gacgaggcgg cccgcggcgc gtcggtctgc
     1741 ctcccctacg tcgcgtcggt cgccgcgtcc ggcgattacg atgtcgaggt gcgcctggca
     1801 cgccggtgcg cctacctgct ggacgacctc gatcgggccg tgagccggcc ggcggctgac
     1861 ggaaggacgc ggatcggcac gggagcgttc tccatcgtct cgtccacgcc cgacgaggtc
     1921 gtcatggagg ccaaccggac gcactacctc gaggcgccgg ccatcgatcg ggtcgtcatc
     1981 cgcccgtacg acgcgctgcg cacggcgttt gcggacatga tgcgcggccg cgtcgatttc
     2041 ctgtgggaag tcgcgcccga cgccgccgag ttcctgggcg atcagcgctc ggtgacggtc
     2101 cgcgcctttc cgggctacta cgcgtacgcc gtgacgatga actcggcgcg gcccgcgctc
     2161 cgcgcggcgg ccgtcaggcg cgcgctggac gcggcggtga atcgcgaggc cctggtgcag
     2221 cagggcctgc gcggccgcgg cgtgccggca gacggccccg tgtggccccg ctactgggcc
     2281 cgcgaccgcg ccgcgccgcc gccggcggcc gatgccgcgg aggcagcggc ccgcctgcgc
     2341 gccgcgggcc cgattgcatt cacctgcctg gtgcccgcca acttcgcgat cctcgagcgc
     2401 atggcgctgc tcgtccagca gcagctcgcc gccttcggcg tcaagatgcg cctcgagtcg
     2461 gcgccgccgg gcgccttcaa ccgccgcctt ctcagcggcg acttcgacgc ggcgatgctg
     2521 tcggtgctgg gcggcccgtc cgccacggtg taccaccggt tctggcattc gccgggcgcc
     2581 gcccggcgct ggaactactg gaactaccgc gacgcgcgcg tcgacgccgc gctcgaggcc
     2641 gcgctcgagg ccccggacga cgaggcgttc aggaaggcca tcggggcctt tcagggcgcg
     2701 tggcgcgaca acccggccgc gatcttcctc gcgtggaacc agaccgtcca ggccgtcagc
     2761 cgtcgcttca cgctgccccc ggggagcgaa acccgcgacg ccatccacgt cctgcacggc
     2821 tggcagccgg agccgcccgc gggacgcacg ccgtgagcac gctgcgcgtc cgcttcgccg
     2881 ccatgctggg cacggcggcc gtcctgcccc tgctgatgta cggcgtggtc tcggtgtact
     2941 cgctgcgcga gggcacgcgg cgcacggtga acgacggcaa cctcaacctc gctcgccagg
     3001 tcggagaaca gctcagccgc tacgtctcga cgaacctcct gatcctcgcc gggctcgccg
     3061 acgacatcgg catcgcggag ctcgatcccg atgagcgcga ccgcatcctg aagaacttca
     3121 tcctgcgctt tcccgagttc cgcgaagtca cggcgctcgc ggcggacggc gcggtcatcg
     3181 ccacgagccg catcggagcg tcgacgctca cgctcccgca agcgggcgga gtgtcgctcc
     3241 acggggtgcg gatctcgccg gtcgtcgtgg acgccgactt cctgccgacg atgctcgtgg
     3301 cggcccccct cgggggcggc gcgcaggccc cgggatggct ggtcggcgag ctgagcatcg
     3361 aggagctctg gcgcctggtg agccgggtcc gggtcggcgc cgccggccac gcgatggtcg
     3421 tcggcccggc gggcgaactg ctggcgcacg gcaacccgga cgagcgcccg gccatcgcgc
     3481 gcggcgcgag cctcgcctcc gatccggtgg tggcggcgct ccggcaagcc gcgcctggcg
     3541 cgccggtcgt gcgggaccgg acgaccgagg ccggggaggt cttcctgtcg gcgggcgtcg
     3601 cgatccccag cctcgggtgg ctggtcatcg tcgaacagcc cgcggccgaa gcgctggccg
     3661 cgtcgcgccg gcaggagcgc gatctgacgg cggccatcgc cctcgggctg ctcgtgatgg
     3721 tgctggcggg cctgatctgg ggccgatcgc tcatcgctcc catccaggag ctcatccgcg
     3781 gcacggaggc catcgccgag ggccggctcg acgagcgcgt ccggatcaca tcgaggtcgg
     3841 agctcggccg tctgggtgac gccttcaaca agatggccgg ccgcctgggc gatctccagg
     3901 aggacgtgag gcggcaggag cggcacgcgg tgttcggacg cgtcgccgcg ggcctcgtgc
     3961 acgacttgtc gcacccgttc aagaacatcc agaacaactg ccggctcatc gtgaagatgc
     4021 acgacgaccc ggaatacctg gagctcttcc gccggacgat cgaccgcgag tgcggccagg
     4081 tgaagcgcgt gttcgaggat ctccgcaacc tcgcgcggcc gatgccgatg gagcggtttc
     4141 ccctcgacct caacaggctg gcggccgacg tcgccgagtc gatgcgcgcc aacgccgaga
     4201 cggcggggct gtcgctggag ctggacctcg cgccgggccc gctgctggtg gagggggatg
     4261 tcttcgcgct gagccgcgtc tgccggaacc tcgtgatgaa cgcgatcgag gcgacgaacc
     4321 cgcgcgggac cgtgaccatc gcgacgcgcg ccgcggccgc ggcggcggtg ctccgcgtgt
     4381 ccgacacggg ctgcggcatc gcgcccgcgc gcctggagac gctcttcgag agcttcgcca
     4441 cgacgaagcg gcaggggctc ggcctggggc tggcgatcgc aaagaagatc gtcgatcagg
     4501 ccggcgggac gatcacggcg tccagcgtcg tcggccgagg ctcggccttc gaggtcacgc
     4561 tggccctggc ggcgccggcc gcctcgggcg gcgaaacccg tcatccccgc gggacggccg
     4621 gaatgcctga ggccgggccg tgaggtgcgg cgagggggag aaggggccgg ccccgcggcc
     4681 ggcccttccg gtcacttgcg cgggaggacg gcgtccttca gctgcttgac cacgcgggcc
     4741 ttgacgacgg tcttcgccgg gatcttgatc ggctcgcccg tcgccggatt gcgcccctgc
     4801 cgggccttgc gcttctgcac gaccagcttg accataccgg gcagcacgaa ctcgcccgag
     4861 cgcttgagct ctttctccga cagcatcgcc agctcgtcga agaactcgcg cgccaccgcg
     4921 cgcttcacct cgaagcggtc cgcgaagtgc gcgaacagct ccaacttgcc catcctgcgg
     4981 gcctctgcca tcgtgactct cctcgaaatg accacgcgcg tttctggtca ccgcaagccg
     5041 gccgcgcgcg caggcggcgg accccggagt gaaagcggaa gtccagaaat cctagatcgc
     5101 gcccactacg ctgtcaatcg gaaggaccgg ctgcaaccgg cggttttcgg ccgctaaatg
     5161 gtaagatccg gcgcgatgtc aagcgcgaag atcgagacgt tccccaaccc gcggccgggg
     5221 cgcgagtacg agatcgccat ctcggccccg gagttcacct ccgtgtgccc gatgacgggc
     5281 ctgccggact tcggcgagat ccgcatctcc tacgtccccg ccgagcggtg catcgaactg
     5341 aagtcgctga agctctacct cgtcgagtac cgcaaccgcg gcatcttcta cgaagccgcc
     5401 accaaccaga tcctggacga cctcgtgacg gcctgccagc cggcgcgcat gaccgtcgtc
     5461 ggcagcttcg cgccgcgcgg cggaatccga acctcgatca cggccaccta cgagagcccg
     5521 tgacagcatc ctcgggatcc gggaggaggc ctgcctcgcc gaggctggct gacgggctgc
     5581 ctggcgaagg cgacggctcg tcgaggcccg ggggccgaag ttcgcccctc ggccaggcgc
     5641 aagcgggcgc cgcgggcgat cggcagcaga gccggcccga gcctggcagc gggcgcgccg
     5701 gcgccgggcc tccaaacgat gacggcgccg ttcgcctgcc catcgacggc acgctggatc
     5761 tgcacgcgtt ccggcctcgc gacgtgaggt cggtggtgga cgactacgtc aacgaggcaa
     5821 gcgccgcggg gctgcgcgcg atcaggatcg cgcacggccg cggaaccggc gtgctgcgag
     5881 gcatcgtcca ggcggccctg gacgcccacc cgctcgtcga ggcgttctgg gacgacaccg
     5941 acgcgcacct tggggcgacg tgggccaggc tggcggccag gcgctaggcg ccagggaagg
     6001 gacgatggca cgaggcaagg cggctttcac cgtcacgctg atcctgggct tcgccctcct
     6061 cgtgccgccg ggcggagcgg cggcgcagga gattccgacg ccggaagcgt tcttcggctt
     6121 caagatgggt gatgacggca ggctcgcgga ctggccgtcc atcgagaagt acttccgggc
     6181 cgtcgccgcg tcctcgccgc gcgtcgagct gatcgaagtc ggcccgtcca ccgaggaccg
     6241 tcgcatcatc gccgccgtga tcagctcgac cgagaacatc agggatcttc cggcgctcca
     6301 gctcgacaac cgcctcctgg cggatcccag gcggctcgag agcgatcggg aggtccgggc
     6361 gattctgtcg ggcgccaaag ccgtcgtggc catcgggtgc agcatccatg cgagcgaggt
     6421 cggcgccacg cagtcggcga gcgagctgct ctacgagctg gcggccgcga gcgacgagcg
     6481 caccgccgca atgctgcgcg acatcatcgt cgtgctgatc ccctcgctca atcccgacgg
     6541 gcacgcgatg gtcgtggact ggcagcggaa gacggcgggc acgccgttcg acggcgggcc
     6601 gatgccgtgg ccgtaccaca agtacgtcgg ccacgatctc aaccgcgacg cgttcatgct
     6661 gaacatggcc gagagccgcg cgctcgcgcg gttcttctcg cgcgagtggc acccgcaggt
     6721 gttcctctcg atgcaccaga tgggatcgaa cgggcccagg ttcttcgtgc cgccgaacgc
     6781 cgatcccatc gattcgaacc aggacccgct catctggcgc gaggccgccc tgctcggcag
     6841 cgcgatggcg atggagctcg agagccaggg caagtccggc gtggtgtcga acgcgatgtt
     6901 cgactatttc tggccggggt acgaggactc ggcgcccctc ggccggaaca ccgtgtgcct
     6961 gctcacggaa gccgcgtcgg cgaggctcgc gagcccgatc gaggtggcgg cgaaggagct
     7021 ccgggggggc gggcgcggcc tgccggaata cgcgccgcag atcaacttcc cgaatccgtg
     7081 gccgggcggc acgtggcgcc tgcgcgacat cgtggactac aacctcgccg cgatgcgcgg
     7141 cctgctctcg gccgtcgccc gctatcgcgg cgagatcctc gcggacttcc acgccatggg
     7201 gcgccgggcc atcgcgcgtg gggccgccga gccgccgttc gcgttcgcga ttccgccgga
     7261 tcagcacgac ccggcggcgg ccgcggcgct cgtcaacctc ctcgccgacg gcggcgtcga
     7321 ggtgcaccag gcgtcggagg ccttcgcggt cggccagacg gaat
//
LOCUS       DAOSIF010000098         7348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9273_length_7348_cov_3.452489, whole genome shotgun sequence.
ACCESSION   DAOSIF010000098 DAOSIF010000000
VERSION     DAOSIF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9273_length_7348_cov_3.452489"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2016)
                     /locus_tag="PLT35_04845"
     CDS             complement(<1..2016)
                     /locus_tag="PLT35_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG28586.1"
                     /translation="MGASRAIAVAACLLGVGVGSCQQGRPAQRYSQKMVILGFDGMEP
                     TLTEQWMAEGRLPNMAGLAAQGGYYRLETSHSPESPTSWASFATGSNAGKHNIFDFLV
                     RDVRTYLPDLGIVKKTMPEFFLDFVPIKMPKVTSIRGGTSFWVTAGRHGVRSSILTVP
                     VTFPPEDVENGEILSGLPLPDIRATIGTFYYFATDLSRYEEGNTEMGGILKRLVMDRG
                     VARTELVGPQNPIIKARQRKILSKGPSLSDADRAELAGLTAREDIRLPVTIHWNRPER
                     RATIEIGGHSIGLAEGEWSKWITLEFKANLLVRMQGMVQLFLVRADHELQLYVSPVNW
                     RPDKPLLPMSSPSGFSADLVDRLGLYRTLGWAEATWPLNEDRIDDKAFMDDLLAAFDD
                     RANVLLHRLDARKWDVLVWVSEATDRVQHMMWRFVDKTHPMYDQAEAARFGDSILRVY
                     QRADRLVGEVLERVEPGTRVIVMSDHGFHSFRQAVNLNTWLVNEGFLAIRGRRAEMKS
                     MNEMFLGSGEFWEKVDWPRTKAYAMGLGQVFINLKGREGQGAVDPADYKAVVDDLVAR
                     LRALTDPKTGRAIMSGVYKRDDIYSGPFFDNAPDIQTGMADGYRVSWQTTLGGSPPGE
                     ILYPNLRKWSGDHCGFDYKTIPGILISNHPLAEAHPRVIDLAPTVLQY"
     gene            2182..2655
                     /gene="ruvX"
                     /locus_tag="PLT35_04850"
     CDS             2182..2655
                     /gene="ruvX"
                     /locus_tag="PLT35_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG28587.1"
                     /translation="MRVLGVDDGLRRIGLAVSDPSGTLATPAGSVPGDGDAARAARTV
                     AAAIARLDRGDEPIGAIVVGLPRRLDGSDTEQTARARALADALGRATGLPVHLQDERL
                     SSREAEDRLARRERDWRARKRRLDAAAAAVILQEFLDWNPEARIRNSECRSRKSE"
     gene            2670..3707
                     /gene="mltG"
                     /locus_tag="PLT35_04855"
     CDS             2670..3707
                     /gene="mltG"
                     /locus_tag="PLT35_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOG28588.1"
                     /translation="MRRIVLALVVTAAAAGAWLAWQQSLRLKEPFQGYAGAEQFVEVP
                     GGAGPRAIGRLLASAGVIRDPITWRLAVWRSGAATRLKAGEYRFAGPLTPAAVVEKLA
                     RGDVFTRSVTFPEGLTIRQMASIYESAGLGPARQFVEAAGQGSLVHAFDPLARDLEGY
                     LFPDTYLLPRRASADDLVRRMVERFERALTEDLRDAARRSGMSIRDLVTLASLVEKET
                     ARADERPLVAAVYRNRLAVQMRLQCDPTVIYALEQQGRFSGNLTRADLAVASPYNTYR
                     YAGLPPGPIAAPGQASLEAAARPAAAPYSYFVSRNDGSHAFASTLREHNRNVQEFQVQ
                     FFRRRPRSASR"
     gene            complement(3779..4654)
                     /locus_tag="PLT35_04860"
     CDS             complement(3779..4654)
                     /locus_tag="PLT35_04860"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOG28589.1"
                     /translation="MITRVGIVAKPGLVALRDDLPRLVAWLEARAVRVCLDHETATLV
                     GTTGVRIVSRDDMSEQVDLVVLLGGDGTLLGMADRIARAGRDVPILGVNFGGLGFLTE
                     IKYDELYPALESVLAGTAAIDARLMLRAVVTRGSEVLRDTPVLNEVALTGGSLSRVVE
                     FGVRVGGNPVATFHADGIIISTPTGSTAYNLSAGGPIVHPAVHALVLNPIAPHTLTNR
                     PVVIPASADVEVRLGLRDREAEAFVTFDGRYGDRLEAHDVVLVQAAPTKARLVRAAAR
                     SYYDVLRLKLRWAER"
     gene            4858..>5508
                     /locus_tag="PLT35_04865"
     CDS             4858..>5508
                     /locus_tag="PLT35_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28590.1"
                     /translation="MVDSRRLLPAVLALFVGSGCAALIYEVVWFQLLELVIGSSSLSL
                     GVLLGTYMGGLCLGSLAFGKVVPPRRHPLRVYALLEAAIGAIGLLVLAGMPLVGSAYA
                     AWAGPGTAGIALRAAAAAVCLLPPTLLMGATLPALARWVEMTPRGVSWLGLFYAGNTA
                     GAVIGSVGAGFYLLRVFDVSIATFVAAATVAFNATATKVAGPAAGREAGPAAAVAVS"
     assembly_gap    5509..5608
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5609..>7348
                     /locus_tag="PLT35_04870"
     CDS             <5609..>7348
                     /locus_tag="PLT35_04870"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOG28591.1"
                     /translation="AGPAAGREAGPAAAVAVSAAASPAAAPRAGAFERKAAYVAIALS
                     GFTALSAEVVWTRLLSLVFGGTTYTFSLILAGILLGIGIGSSAGSALARSLTRPAAAL
                     GWCQAGLCAAIAWGAAMLCGSLPYWPIDPSISMSPAFNFQLDAARCLWTVLPGALLWG
                     ASFPLALAAVARFGGDQARVVGGMYAANTLGAIVGSLGAGLLVARFGSQHVHQLMIAV
                     AAAAALLMLLPGFGAPAAAPRRAAASWLAAAALVVAIAGGAAGAGLAVPPVPGLLVGY
                     GRFSATWVNQAEFIYVGEGLNASVAVSRLPSGVLNYHNAGKIQASSEPQDMRLQRMLG
                     HLTTLVPDRPASVFVIGFGAGVTAGAVAIDPSVERVTIAEIEPLVIKTVSAYFSAHNH
                     DVVNNPKVRIHIDDGRHFLLTTDERFDAITSDPLDPWVKGAAMLYSREFFQLVRRRLN
                     PGGIVTLFVQLYSSSEEAVKSEIATFFEAFPDGIVFGNTYNGAGYDLVLLGQAAPGPI
                     DIDRIEARLRSPGFGRVAASLGEVGFGSAVSLLATYAGEAGDLRPWLAGAAINRDRNL
                     RLQYLAGMGVNLRV"
BASE COUNT          960 a         2561 c         2634 g         1093 t
ORIGIN      
        1 atactgcagc accgtcgggg cgaggtcgat cacccgcggg tgcgcctcgg caagcgggtg
       61 gttcgagatc aggatcccgg gaatggtctt gtagtcgaac ccgcagtggt cgccgctcca
      121 cttccgcagg ttcggataga gaatctcccc gggcggcgac ccgccgagcg tcgtctgcca
      181 cgacacgcgg tagccgtccg ccattcccgt ctggatgtcc ggcgcattgt cgaagaacgg
      241 gcccgagtag atgtcgtcgc gcttgtagac gccgctcatg atcgcccggc cggtcttcgg
      301 gtccgtgagg gcccgcagcc gcgcgacgag atcgtccacg acggccttgt agtcggccgg
      361 atcgaccgcg ccctggcctt cacggccttt caggttgatg aacacctgcc cgaggcccat
      421 cgcgtacgcc ttggtccgcg gccagtccac cttctcccag aactcgccgc tgccgaggaa
      481 catctcgttc atgctcttca tctcggcgcg ccggcccctg atggccagga acccctcgtt
      541 gaccagccac gtgttgaggt tcacggcctg gcggaacgaa tggaagccgt gatcggacat
      601 cacgatcacg cgcgtgcccg gctccacgcg ctcgagcacc tcgccgacca gccgatcggc
      661 gcgctggtac acgcggagga tcgaatcgcc gaacctggcg gcttcggcct ggtcgtacat
      721 cgggtgcgtc ttgtccacga agcgccacat catgtgctgg acgcggtcgg tcgcctcgga
      781 cacccacacg agcacatccc acttccgcgc gtcgaggcgg tgcaacagga cgttggcccg
      841 gtcgtcgaag gcggccagga gatcgtccat gaacgccttg tcgtcgatgc ggtcctcgtt
      901 gagcggccag gtggcctcgg cccagccgag cgtacggtac agcccgagcc gatccacgag
      961 gtccgccgag aagcccgaag gcgacgacat gggcagcagc ggcttgtccg ggcgccagtt
     1021 caccggcgac acgtagagct ggagctcgtg gtcggcccgc accaggaaca gctgcaccat
     1081 gccctgcatg cgcaccagca ggttcgcctt gaactcgagg gtaatccact tgctccattc
     1141 gccctcggcg agcccgatgc tgtggccccc gatctcgatc gtcgcccgcc gctccggccg
     1201 gttccagtgg atcgtgacgg ggagccggat atcctcgcgc gccgtcagcc ccgccagctc
     1261 cgcgcggtcg gcgtccgaga gcgacgggcc cttcgacagg atcttgcgct gccgggcctt
     1321 gatgatcggg ttctgcgggc cgacgagctc cgtcctggcg acgcccctgt ccatcacgag
     1381 gcgtttcagg atgcccccca tctcggtgtt gccttcctcg taccgcgaca ggtccgtcgc
     1441 gaaatagtag aaggtcccga tggtcgcgcg gatgtccggc agcggcagcc ccgacaggat
     1501 ctcgccgttc tcgacgtcct cgggcgggaa cgtcaccggc acggtcagga tgctggagcg
     1561 aacgccgtgc cgcccggcgg tgacccagaa ggaggttccg ccgcggatgg aggtgacctt
     1621 gggcatcttg atcggcacga agtcgaggaa gaactcgggc atcgtcttct tgacgatgcc
     1681 gagatccggc aggtaggtcc tcacgtcgcg cacgaggaag tcgaagatat tgtgcttgcc
     1741 ggcgttggag ccggtggcga acgaggccca cgacgtgggc gactccggcg agtggctggt
     1801 ctcgaggcgg tagtagccgc cctgcgccgc caggcccgcc atgttcggca gccggccctc
     1861 tgccatccac tgctcggtca gcgtcggctc catgccgtcg aacccgagga tgaccatctt
     1921 ctgggagtac cgctgcgcgg gacggccctg ctggcacgat ccgacgccca cgcccagcag
     1981 gcaggcggcg acggcgatgg cgcggctcgc tcccacggct gcccccttcc cggcacggcg
     2041 gacccgggac tcgggagcag tatagcgcgc gtgcggtagc ggatcgacgc cgcgcagcgc
     2101 cccgccaggg ccgccgttcc cgcggcggcg gcgagagggt acactggagg gcgcggaacg
     2161 gcatcggagg cgacgcagcg gatgcgcgtg ctgggcgtgg acgacggact gcggcggatc
     2221 ggcctggccg tgagcgaccc ctcgggcacg ctcgcgacgc ctgccggaag cgtcccgggc
     2281 gacggcgacg ccgcccgggc cgcgcggacc gtcgccgcgg ccatcgccag gctcgatcgt
     2341 ggcgacgagc cgatcggcgc cattgtcgtc ggcctgcctc gccgcctcga cggctccgac
     2401 accgagcaga ccgcccgcgc cagggcgctg gccgacgctc ttgggcgcgc aaccggcctg
     2461 cccgtgcacc tgcaggacga gcgcctcagc agccgcgagg ctgaggaccg cctggcgcgc
     2521 cgcgagcggg actggcgcgc gcgcaagcgc cggctcgacg cggcggcggc ggcggtgatc
     2581 ctgcaggagt tcctggattg gaacccggaa gccaggattc ggaattcgga atgcagaagt
     2641 cggaagtcgg aatgaggtgc gtgggaggga tgagacggat cgtcctggcc cttgtcgtga
     2701 cggccgccgc cgccggcgcc tggctggcgt ggcagcagtc cctccggctg aaggagccgt
     2761 tccagggata cgccggcgcc gagcaattcg tcgaggtgcc cggcggggcc ggcccccgcg
     2821 cgattggccg gctgctggcg agcgccgggg tgatccggga cccgatcacg tggcgcctgg
     2881 cggtctggcg gagcggggcc gccacgaggc tgaaggcggg cgaataccgg tttgcggggc
     2941 cgctgactcc cgctgccgtc gtcgagaagc tcgcgcgcgg cgatgtgttc acgcggagcg
     3001 tgacgttccc ggaagggctg acgatccggc agatggcgtc gatctacgaa tcggccggcc
     3061 tgggaccggc gcggcaattc gtggaagcgg ccgggcaggg cagcctcgtc cacgccttcg
     3121 acccgctggc ccgcgatctc gaaggctact tgttcccgga cacgtacctg ttgcccaggc
     3181 gcgcctcggc cgacgatctc gtccggcgca tggtggagcg attcgagcgg gcgctgacgg
     3241 aggacctgcg cgacgcggcc cgccgcagcg gcatgtcgat ccgggatctg gtgacgctcg
     3301 cgtcgctcgt ggagaaggaa accgcgcgcg ccgacgagcg gccgctggtc gcggcggtct
     3361 accgcaaccg cctggccgtg cagatgaggc tgcagtgcga tcccacggtg atctacgcgc
     3421 tcgagcagca ggggcgcttc agcggcaacc tcacgcgggc ggacctcgcc gtcgcgtccc
     3481 cgtataacac ctaccgctac gcggggctcc ccccagggcc gattgccgcg cccgggcagg
     3541 cgtcgctgga ggcggcggcg cgtccggcgg cggcgcccta ctcgtacttc gtcagccgca
     3601 acgacggttc ccacgccttc gcgtcgacct tgcgcgagca caatcggaac gtccaggagt
     3661 tccaggtgca gttcttccgc cggcgccccc gctccgcctc gcgctagccg gttccgcacc
     3721 ccgcactccg gattgccgcc ttcgccgcgg ctggctgaac tccggattcg gtcttcctct
     3781 atctctccgc ccagcgcagc ttcagccgga gcacgtcgta gtagctgcgg gccgccgccc
     3841 ggacgagccg ggctttcgtg ggggcggcct ggacgaggac gacgtcgtgc gcctcgaggc
     3901 ggtcgccgta gcgcccgtcg aacgtgacga aggcctcggc ctcgcggtcc cgcaggccca
     3961 ggcggacctc gacgtcggcc gaggccggga tgaccaccgg ccggttcgtc agcgtgtgcg
     4021 gcgcgatagg gttcagcacg agggcgtgca cggcgggatg gacgatcggc ccgccggcgg
     4081 agaggttgta ggcggtcgag ccggtcgggg tggagatgat gatgccgtcc gcgtggaacg
     4141 tcgcgacggg attgccgccg acgcgcacgc cgaactcgac gacgcgcgac agcgacccgc
     4201 cggtgagggc gacttcgttg agcaccggcg tatcgcgcag cacctcgctg ccgcgcgtga
     4261 cgacggcgcg cagcatcagc cgcgcgtcga tggccgccgt gccggcgagc actgactcga
     4321 gcgcgggata cagctcgtcg tacttgatct cggtcaggaa cccgagcccg ccgaagttca
     4381 cgccgaggat cggcacgtcg cggcccgccc tggcgatccg gtcggccatg ccgagcaggg
     4441 tgccgtcgcc gccgagcagc accaccaggt cgacctgctc cgacatgtcg tcgcgcgaca
     4501 cgatgcggac gccggtcgtg ccgacgagcg tcgccgtttc gtggtcgagg cacacccgga
     4561 cggcgcgggc ctccagccac gccaccagcc gcggcaggtc gtcgcgaagc gcgaccaggc
     4621 cgggcttggc gacgatgccg acgcgtgtga tcatcgcggt cccctccgtg cagcgccatt
     4681 ctagcagcgc gcgccgccgc ggggtggccg gggcaggccg acgagccggc cccggagatc
     4741 cggcccgagg atcgaacgga agatccgctt gccgcaggcg cggcgagccc gtagactggc
     4801 ggcgctccgg tcgccccgtg cggggcgtgg ccgccgcgaa cggacaggat gccgcccatg
     4861 gtcgactcgc gccgacttct tcccgcggtc ctcgcgctct ttgtcgggag cgggtgtgcc
     4921 gcccttatct acgaggtcgt ctggttccag ctgctcgagc tggtcatcgg ctcgtcgtcg
     4981 ctgtcgctgg gcgtgctgct cggcacctac atgggcgggc tctgcctcgg cagcctcgcg
     5041 ttcgggaagg tcgtgccgcc tcggcgccat ccgctgcggg tgtatgcgct cctcgaagcg
     5101 gcgatcggcg ccatcggcct gctggtgctg gccggcatgc cgctggtggg cagcgcgtac
     5161 gcggcatggg cgggccccgg cacggccggc atcgcgctgc gcgccgcggc cgcggccgtg
     5221 tgcctgctgc cgcccacgct gctcatgggc gcgaccctgc cggcgctggc gcgctgggtg
     5281 gagatgacgc cgcgcggcgt gtcgtggctc gggctgtttt acgcgggcaa cacggcgggg
     5341 gccgtcatcg ggagcgtggg cgcgggcttc tacctgctcc gtgtgttcga cgtgagcatc
     5401 gcgaccttcg tcgccgcggc caccgtggcg ttcaacgcga cggcgacgaa ggtcgccggc
     5461 ccggcagccg gccgcgaggc ggggccggcg gcagccgttg ccgtcagtnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ccggcccggc agccggccgc gaggcggggc
     5641 cggcggcagc cgttgccgtc agtgccgcgg cctcgcccgc ggcggcgccg cgggcggggg
     5701 cgttcgagcg caaggcggcc tacgtggcca tcgcgctctc gggcttcacc gccctgtccg
     5761 ccgaggtggt ctggacccgg ctgctgtcgc tcgtcttcgg cggaaccacc tacaccttct
     5821 cgctgatcct cgccgggatc ctcctgggga tcggcatcgg cagcagcgcc ggctcggccc
     5881 tggcgcggag cctcacgcgt ccggccgcgg ccctcggctg gtgccaggcc gggctgtgcg
     5941 ccgccatcgc gtggggcgcg gcgatgctgt gcggctcgct gccgtactgg cccatcgacc
     6001 cctcgatctc gatgagcccg gcattcaact tccagctcga cgccgcgcgc tgcctctgga
     6061 ccgtgctgcc tggggccctg ctgtggggcg cgagcttccc gctcgcgctg gcggcggtgg
     6121 cgcgcttcgg cggcgaccag gcgcgcgtcg tcggcgggat gtacgccgcg aacacgctgg
     6181 gtgcgatcgt cggatcgctc ggcgcgggcc tcctggtggc ccggttcggc agccagcacg
     6241 tgcaccagct gatgatcgcc gtggcggccg ccgcggcgct gctgatgctg ctccccgggt
     6301 tcggcgcccc ggccgccgcg ccccggcgcg ccgcagcgtc ctggctggcc gccgccgcgc
     6361 tcgtcgtcgc gatcgcaggc ggggcggcgg gggccggcct cgcggtgccg cccgtgccgg
     6421 ggctgctcgt cggctacggc cgcttctcgg ccacgtgggt caatcaggcg gagttcatct
     6481 acgtcggaga agggctcaac gcctcggtgg ccgtgtcgag gctgccgagc ggcgtgctca
     6541 actaccacaa cgcgggcaag atccaggcgt ccagcgagcc ccaggacatg cggctccagc
     6601 ggatgctcgg ccacctgacc acgctggtgc ccgaccggcc cgcgtcggtc ttcgtgatcg
     6661 ggttcggcgc gggcgtcacg gccggcgccg tggcgatcga cccgtcggtc gagcgcgtga
     6721 cgatcgccga aatcgagccg ctcgtcatca agaccgtgtc ggcgtacttc agcgcccaca
     6781 accacgacgt cgtcaacaac ccgaaggtcc ggattcacat cgacgacggg cgccacttcc
     6841 tgctcacgac cgacgagcgg ttcgacgcga ttacgtcgga tcccctggac ccctgggtca
     6901 agggcgccgc gatgctgtat tcgagggagt tcttccagct cgtcaggcgg cggttgaacc
     6961 cgggcggcat cgtgacgctg ttcgtgcagc tctactcgag cagcgaggag gccgtgaaga
     7021 gcgagatcgc cacgttcttc gaggccttcc cggacggcat cgtgttcggc aacacgtaca
     7081 acggcgcggg ctacgacctg gtgctgctgg ggcaggccgc gccagggccg atcgacatcg
     7141 atcggatcga ggcgcggctc aggagccccg gtttcgggcg ggtcgccgcg tcgctcggcg
     7201 aggtcgggtt cgggtcggcc gtcagcctgc tggcgacgta cgccggcgag gccggcgatc
     7261 tccggccttg gctggcgggg gcggccatca accgcgaccg caacctgcgg ctgcagtacc
     7321 tggcggggat gggcgtcaac cttcgcgt
//
LOCUS       DAOSIF010000099         7281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9352_length_7281_cov_3.866039, whole genome shotgun sequence.
ACCESSION   DAOSIF010000099 DAOSIF010000000
VERSION     DAOSIF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9352_length_7281_cov_3.866039"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLT35_04875"
     CDS             complement(<1..960)
                     /locus_tag="PLT35_04875"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOG28592.1"
                     /translation="MNASISFVRATARLGVCLAFLAAPASVIAQGGGWQVQNLPTLPS
                     GSTYHFTAVSAVSETDAWIAGYIYPSREVFVARTEDGGAAWQIVFQSATWPTINRMTF
                     LPEVGFVAGTFDLFKSTTDGGVTWNQEMGNLPVPPGEWHPVGPGGHIYGLAVADSTHI
                     WTAGWDGAGAGVVFHRKPERPQSDPPNYNRPWWLELGTNNVGMYGIAAASQNVAWAVG
                     YQGAIWKTSDGGNGWGPQASGTGASLYDVVAIDANTLWAVGEAGTILKTTDGGANWVP
                     QTSNTTENLRRIAALDASTAWAVGTAGVILHTTDGGTTWEPQWS"
     gene            1216..1431
                     /locus_tag="PLT35_04880"
     CDS             1216..1431
                     /locus_tag="PLT35_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28593.1"
                     /translation="MAVRIESRGGGDDDSGRTHRVVTSESTLRADDAVTGSFQGWNKA
                     GEAIRGTYAGRRLKQGIDRFRSAPGRV"
     gene            1560..3011
                     /locus_tag="PLT35_04885"
     CDS             1560..3011
                     /locus_tag="PLT35_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOG28594.1"
                     /translation="MSKAIEGLKPAGVWKAFADLSAIPRPSKHEAAAAAFVIDTAKRL
                     GLEWAQDAAGSVVVRKPASKGYEGRPMVCLQGHLDMVPEKRPDKQHDFLKDPITLVRK
                     GNVMMADGTTLGADNGVAVATSLAIMADTSLAHGPLEFLFTVDEETGLTGANNLQPGF
                     LKSTILLNLDSEEEGELYVGCSGGRDTMGTWALDTEPAPAGTVGLLVKVGGLRGGHSG
                     LEIDKGRGNAIKILNRALMKLAPAGARLASIDGGNKRNAIPRDASALVFVPKPKAAEA
                     KAIVAAVNDVCRAELASVEPSLAVTAEPFKGRRKVVRKGLQKKLTMALAALPHGVIKM
                     SADIPGLVETSTNVGVITTGKKDISVATSQRSSVASEIAEIADAVRAVFELGGAKVSG
                     SDGYPGWKPNLESPILKLAIATYKKLYGKEPAVKAVHAGLECGIIGEKYPGMDMVSFG
                     PTLEGVHSPDEKIHIDTVQKYWDFLLALLQNVT"
     gene            complement(3210..4070)
                     /locus_tag="PLT35_04890"
     CDS             complement(3210..4070)
                     /locus_tag="PLT35_04890"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG28595.1"
                     /translation="MFGRQTQGSVVCASCGRLVGVNDEVCYTCGRRNPGLWGFSPLLR
                     RLGNDLGFVPLVTWGSAGLYAAMLLVSGSGIRMGGMFSFLAPSTKSLFLFGASGGLPV
                     FRYDRWWTVLSAGWLHAGLLHILFNMLWVRQLGPACAELYGAGRMIIIYTVAGVVGFA
                     ASSLAWLRLGGIPVLGGAGFTVGASAPVFGLLGALVLYGQRSGSSMTKREGLHYALML
                     GLFGLIMPGVDNWAHAGGFAGGWLAAKLLDPLKPERIDHLAAAALCVLATIGAIAASI
                     VHGLALFGGR"
     gene            4317..5753
                     /locus_tag="PLT35_04895"
     CDS             4317..5753
                     /locus_tag="PLT35_04895"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA Peptidase M64"
                     /protein_id="HOG28596.1"
                     /translation="MRRTPLGAIALVLLLSPASPRLGSATPYDDLFTADTMRIDYSHT
                     GGRGIEAVSVDRIVNDGPWAGSRARLVDDTNLGKYLFEVVDRRTNRVVYSRGFASLFG
                     EWETTPEARDTYRTFGGSLRFPWPRQPVQVVLKKRDRLNAFHEFWSTLVDPASRAVNR
                     ARLAPTGRVWTLWENGPPESKVDLLLIGDGYTEAELPKFHADAKRLVDRMFTVEPYKS
                     RRSDFNVRAIDVAAAESGVHNPRTGDMRRSRVGTEYNIFDSERYALSPDNRGWRDVAS
                     EAPYDAVGILINNRYYGGGGIFNDQATVAVDSGSAEYIFIHEFGHHFAGLGDEYYTSD
                     VAYETGAAEKVEPWEPNVTALLDPSALKWKDLVDPGTPLPTPWDKAEYEKRSAEIQAE
                     RRRLRASAAPESAMDALFARQLDFEKTFLSSMKYSGRVGAFEGASYEPTGLYRPETDC
                     IMFSRNPAGFCRVCRRAIERVIDLHTGR"
     gene            5761..>7281
                     /locus_tag="PLT35_04900"
     CDS             5761..>7281
                     /locus_tag="PLT35_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HOG28597.1"
                     /translation="MPWVQVYDPLGRAWLSTAAAALPTGLLLLALAALRWPAHRAAAL
                     GLAAAVAVSVGVFGMPAPAALATALYGAAYGLFPIGWIVLCAIFLYNLSVRSGTFDVV
                     KRSVARLSGDRRVQALVIAFSFGAFIEGCAGFGAPVAISAALLAGLGFAPRYAASLAL
                     IANTAPVAFGSIATPIFTLAAVTGLPATALGAMAGRQLPFVSILVPAWLIVTMSGWRG
                     LRGVWPAVAACGGAFALVQFAWSNFVGPELVDITAALASLAAVGLLCARWQPAGAPRR
                     GRSPAADAGNARLGPRELLRGWLPWILLTVCVTLWGAPPVKAALSAPAWSAPRVEIPA
                     LHRRVFRDFPVVPTPVDRARLADPDYRRTRAEAAVFTINWASATGTSVLAAALGTILF
                     LRMRRRDVADVARATWRQMRVPLLTISTMMALGFITRYGGTDATLGLAFTKTGWAYPF
                     FAAMLGWLGVAITGSDTSSNVLFGSLQKITAQQLGLDPVLIAASNSTGGVMGKMVAA"
BASE COUNT         1102 a         2528 c         2602 g         1049 t
ORIGIN      
        1 ggaccactgc ggctcccacg tcgtgccgcc gtcggtcgtg tgcagaatga cgccggctgt
       61 cccgacggcc catgccgtgc tggcgtcaag tgcggcgatg cgccgcagat tctcggtggt
      121 gttcgaggtc tgcggaaccc agttcgcacc gccgtcggtg gtcttcagga tcgtgcccgc
      181 ctcgccgacg gcccacaggg tgtttgcgtc aatcgcgacc acgtcgtaga gcgacgcgcc
      241 ggtgcccgag gcctgcggcc cccagccgtt gccgccgtcg gaggtcttcc agatcgcgcc
      301 ctggtagccg acggcccagg cgacgttctg gctcgccgcg gcgatgccgt acatgccgac
      361 gttgttcgtc cccaactcga gccaccacgg gcggttgtaa ttgggcgggt cggactgcgg
      421 gcgttccggc ttccggtgga atacgacgcc ggcgccggcg ccgtcccagc ccgccgtcca
      481 gatgtgcgtg ctgtcggcga ctgccaggcc atagatgtgg ccgccgggac cgacggggtg
      541 ccactcgccc gggggaacgg ggagattgcc catttcctgg ttccaggtga cgccaccgtc
      601 cgttgtcgac ttgaacaggt cgaaggtccc tgcgacgaac cccacctcgg gcaggaacgt
      661 catccggttg atggtgggcc acgtcgccga ctggaagacg atctgccacg cagcgccgcc
      721 gtcctcggtc cgcgccacga agacctcgcg gctcgggtag atgtagcccg cgatccatgc
      781 gtctgtctcg cttaccgcgg aaacagccgt gaaatggtac gtgctgccgc tcggcaacgt
      841 tggcaggttc tgcacctgcc agccgccgcc ctgggcgatg acgctggctg gtgcggcaag
      901 aaacgcgagg cacacgccga gtcgggccgt cgcgcggacg aacgagatgg aggcgttcat
      961 gattcacctc tcgaggccga caaggcggcc tctgctggac gccgggcgca cctcgaagga
     1021 gatcgggcgc acaccggcgc atgacgacgc gggcggctga tactggaccc aaacagaacc
     1081 gaagtcaagc cgggggtgtg acatggggag ctggccgggg attggacgtt tcacgccttc
     1141 atgtgggctc gccgcctcga tcccgagtgg gacagccgct gcgctcccca gacatgcggc
     1201 cagcagcagc gcacgatggc cgtcaggatc gagtctcgcg ggggcggcga cgatgactcc
     1261 ggcaggacgc accgcgtcgt gacgtcggag tccacgcttc gcgcggatga cgccgtgacc
     1321 gggtcgtttc agggctggaa caaagccggc gaggcgatca ggggcacgta tgccggcagg
     1381 cggctgaagc agggcataga ccggttccgg agcgcccctg gcagggtgtg aggctcacgc
     1441 aggaaagcag cgtcagtccc gccggacaag gccggcaggc ctcgccccga gtcccgaatc
     1501 ccgagccccg gtctctgtgc tagagtcgat gagcctcgcg tgtcaaagga gccagtccca
     1561 tgtcgaaagc catcgaaggg ttgaagcccg ccggagtgtg gaaggcgttc gcggatctgt
     1621 cggcgatccc ccgtccgtcg aagcacgagg ccgccgcggc ggcattcgtc atcgacacgg
     1681 cgaagcggct cgggctggaa tgggcccagg acgctgccgg ctcggtcgtc gtccgcaagc
     1741 cggcatcgaa ggggtacgag gggcggccga tggtgtgcct gcagggacac ctggacatgg
     1801 tgcccgagaa gcggcccgac aagcagcacg acttcctgaa ggatcccatc acgctcgtcc
     1861 gcaagggcaa cgtcatgatg gccgacggca cgacccttgg cgccgacaac ggcgtggcgg
     1921 tggccacgag cctggccatc atggccgaca cgtccctcgc gcacgggccg ctcgagttcc
     1981 tcttcaccgt cgatgaggag accgggctca ccggcgccaa caacctccag cccggctttc
     2041 tgaagagcac catcctgctg aacctggact cggaggagga aggcgagctg tacgtcggct
     2101 gctcgggagg ccgcgacacg atgggcacgt gggccctgga cacggaaccg gccccggcgg
     2161 gcacggtcgg cctgcttgtc aaggtgggcg gcctgcgggg cggccactcg ggcctcgaga
     2221 ttgacaaggg ccgcggcaac gcgatcaaga tcctgaaccg ggccctgatg aagctggccc
     2281 ccgccggcgc caggctggcc tcgatcgacg gcgggaacaa gcgcaacgcc atcccgcgcg
     2341 acgcgtcggc gctggtgttc gtgccgaagc cgaaggccgc cgaggcgaag gcgatcgtgg
     2401 ccgccgtcaa cgacgtgtgc cgcgcggaac tggcgtcggt cgagccgtcg ctcgccgtca
     2461 ccgccgagcc gttcaagggc cgcaggaagg tcgtgaggaa aggcctgcag aagaagctga
     2521 cgatggcgct cgccgcgctg ccgcacggcg tgatcaagat gagcgcggac atcccggggc
     2581 tggtggagac gtccacgaac gtcggcgtca tcacgaccgg caagaaggac atctcggtcg
     2641 cgacgagcca gcgcagctcg gtggcgtctg agatcgcgga gatcgccgac gccgtcaggg
     2701 ccgtcttcga gctcggcggc gcgaaggtga gcgggtccga cggatacccc ggctggaagc
     2761 cgaacctcga gtcgccgatc ctcaagctcg cgatcgccac ctacaagaag ctgtacggca
     2821 aggagccggc cgtgaaggcc gtccacgccg ggctcgagtg cgggatcatc ggcgagaagt
     2881 acccggggat ggacatggtg tcgttcggcc cgacgctgga aggcgtccac tcgccggacg
     2941 agaaaataca catcgacaca gtgcagaagt actgggactt cctgctggcg ctgctgcaga
     3001 acgtcacgta atcggggctc gggactcggc ggcccttcga cccggcgcct cgcggctcgc
     3061 ccggagcgcg ccgcgccggc gcagggcggg ggccaggcgc cgggcgccgg ccgggcgcgg
     3121 cacgcgagcc gcgcccaccg gccggccgcg ggcgttccgg gccctgaagt ccgggttccg
     3181 gcttctggat tctgacggct gacttccggc taccgtcccc cgaacagcgc cagcccgtgc
     3241 acgatcgacg cggcaatcgc gccgatcgtc gccagcacgc acagcgcggc ggccgccagg
     3301 tgatcgatgc gctcgggctt gagcggatcg aggagcttgg cggccagcca gccgcccgcg
     3361 aagccgccgg cgtgggccca gttgtccacg ccgggcatga tcaggccgaa caggccgagc
     3421 atcagggcgt agtgcaggcc ttcgcgcttc gtcatgctgc tgccggagcg ctgaccgtag
     3481 aggacgaggg cgccgaggag gccgaagacg ggagcggacg cgcccaccgt gaatccggcc
     3541 ccgccgagaa cggggatgcc gccgagcctg agccacgcca gcgagctcgc ggcgaagccc
     3601 acgacgccgg cgacggtgta gatgatgatc atgcggccgg cgccgtacag ctcggcgcag
     3661 gccggcccga gctgacgcac ccacagcatg ttgaagagga tgtgcagcag cccggcgtgc
     3721 agccagccgg cgctcagcac ggtccaccac cggtcgtagc ggaagaccgg gagcccgccg
     3781 ctcgcgccga acaggaacag gctcttcgtg ctcggcgcca ggaacgagaa catgcccccc
     3841 atgcggatgc ccgatcccga gacgagcagc atggcggcgt agagcccggc cgagccccag
     3901 gtcacgagcg gcacgaaccc caggtcgttc ccaaggcgcc ggagcagcgg cgagaagccc
     3961 cacagccccg ggttcctgcg gccgcaggtg tagcagacct cgtcgttcac gccgacgagc
     4021 cgcccgcagg acgcgcagac gaccgaccct tgcgtctgtc ttccgaacat gggacctctg
     4081 gggagatcat atcgtgatcg gggcttgggg ctcgggactc ggggcttggg gctcggggct
     4141 cgggaagaca ggggcaaggg acaaggggca cggggaaagc tcgccgcgcc gaagctggcc
     4201 gttcggctcg cggctgctcg ctcagggcaa gccggcaggc cgttcagcga aggcgggggc
     4261 tgcactatac tggcgcggtt ctgcggaacg ctgcacgctc cagccggagg cttcgcatgc
     4321 gacggacccc gctcggcgcc atcgccctcg tcctgctcct ctcgccggcc tcccccaggc
     4381 tcggctccgc gacgccctac gacgatctgt tcaccgccga cacgatgcgg atcgactact
     4441 cgcacaccgg ggggcgcggc atcgaggccg tctccgtcga tcgcatcgtc aacgacggcc
     4501 cctgggccgg aagccgcgcg cgcctcgtgg acgacacgaa cctcggcaag tacctcttcg
     4561 aggtcgtcga tcgccgtacg aaccgcgtcg tctactcgcg cgggttcgcc agtctcttcg
     4621 gcgagtggga aacgacgccg gaagcgcggg acacgtaccg cacgttcggc gggtccctgc
     4681 gcttcccgtg gccgcggcag ccggtgcagg tcgtgctgaa gaagcgcgac cgcctgaacg
     4741 cgttccacga gttctggtcc acgctcgtcg atccggcgtc gcgcgcggtc aaccgggcgc
     4801 gcctggcgcc caccggccgg gtgtggacgc tgtgggagaa cggtccgccc gagtcgaagg
     4861 tggacctgct gctgattggc gacggctaca cggaggccga gctgccgaag ttccacgccg
     4921 acgcgaagcg gctggtggat cggatgttca ccgtggagcc gtacaagagc cggcggagcg
     4981 acttcaacgt ccgggccatc gacgtggcgg ccgccgagag cggcgtccac aacccccgca
     5041 cgggggacat gcgccggtcg cgcgtcggca ccgagtacaa catcttcgac agcgagcggt
     5101 acgcgctgag ccccgacaac cgcggctggc gcgacgtcgc gtcagaggcg ccgtacgacg
     5161 ccgtcgggat tctgatcaac aaccgctatt acgggggcgg agggatcttc aacgaccagg
     5221 ccaccgtcgc cgtcgacagc ggttcggccg agtacatctt catccacgag ttcggccacc
     5281 acttcgcggg gctcggggac gagtactaca cgtccgacgt cgcctacgaa acgggcgcgg
     5341 cggagaaagt cgagccgtgg gagccgaacg tcaccgcgct cctggatccg tcggccttga
     5401 agtggaagga cctcgtcgat cccggcacgc cgctgccgac gccctgggac aaggcggagt
     5461 acgaaaagcg gtcggcggag atccaggccg agcggcgccg tctgcgggcg agcgcggcgc
     5521 ccgagtcggc gatggacgcc ttgtttgcgc ggcagctcga cttcgagaag acgttcctgt
     5581 cgtccatgaa gtactcgggc agggtcggcg ccttcgaagg cgcgtcgtac gagcccaccg
     5641 ggctctaccg gcccgagacg gactgcatca tgttctcgcg caacccggcc gggttctgcc
     5701 gggtctgccg gcgcgcgatc gagcgcgtca tcgacctcca cacgggaagg taggctgccg
     5761 atgccctggg tccaggtcta cgatccgctg gggcgggcgt ggctcagcac ggcggccgcg
     5821 gcgctgccga ccggcctgct gctcctcgcg ctggcggcgc tgcgctggcc ggcgcaccgg
     5881 gctgccgcgc tgggcctcgc cgcggcggtg gcggtgtcgg tcggggtctt cgggatgccg
     5941 gcgccggcgg cccttgcgac ggccctgtac ggcgcggcct acggtctgtt ccccatcggc
     6001 tggatcgtcc tctgtgcgat tttcctctac aacctcagcg tcaggtccgg caccttcgac
     6061 gtcgtcaagc gctcggtcgc gcggctctcg ggcgaccggc gcgtgcaggc cctggtgatc
     6121 gcgttctcct tcggcgcctt catcgagggg tgcgccgggt ttggcgcgcc cgtggccatc
     6181 tcggctgccc ttctggccgg cctcgggttc gcgccgcgct atgcggcgtc gctggccctc
     6241 atcgccaaca cggcgccggt cgcgttcggc tcgattgcca cgccgatctt caccctcgcc
     6301 gcggtcaccg gcctcccggc aaccgcgctc ggcgcgatgg ccgggcgcca gctgccgttt
     6361 gtctcgatcc tcgtgcccgc ctggctcatc gtcacgatga gcgggtggcg gggcctgcgc
     6421 ggcgtgtggc cggcggtggc ggcctgcggc ggcgcgttcg ccctcgtgca gttcgcctgg
     6481 agcaacttcg tcggacccga gctggtggac atcaccgcgg ccctcgcctc gctcgccgct
     6541 gtcgggctgc tgtgcgcgcg gtggcagccc gccggcgccc cgcgccgcgg ccgctccccc
     6601 gccgccgacg cgggcaacgc ccgcctcggg ccgcgcgaac tgctccgggg atggctgccc
     6661 tggatcctgc tgacggtctg cgtgacgctg tggggcgccc cgcccgtcaa ggccgccttg
     6721 agcgcgccgg cgtggagcgc gccgcgcgtc gagatcccgg ccctgcaccg gcgcgtgttc
     6781 cgcgacttcc ccgtcgtgcc gacgccggtc gaccgcgcgc ggctcgccga ccccgattac
     6841 cggcgcaccc gggcggaggc ggcggtcttc acgatcaact gggcgtcggc gacgggcacc
     6901 agcgtcctcg ccgctgcgct cggcacgatc ctgttcctgc ggatgcggcg ccgggacgtt
     6961 gcggacgtcg cccgcgccac gtggcgccag atgcgggtgc cgctgctcac gatctcgacc
     7021 atgatggcgc tggggttcat cacgcgctac ggaggcaccg acgccacgct gggcctggcc
     7081 ttcacgaaga ccgggtgggc ctacccgttc ttcgcggcca tgctcggctg gctgggcgtg
     7141 gccatcacgg gttccgacac cagctccaac gtgctgttcg gcagcctgca gaagatcacg
     7201 gcgcagcaac tgggcctcga cccggtcctc atcgcagcct cgaacagcac cggaggcgtg
     7261 atgggcaaga tggtcgccgc c
//
LOCUS       DAOSIF010000100         7271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9366_length_7271_cov_4.318736, whole genome shotgun sequence.
ACCESSION   DAOSIF010000100 DAOSIF010000000
VERSION     DAOSIF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9366_length_7271_cov_4.318736"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLT35_04905"
     CDS             <1..398
                     /locus_tag="PLT35_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOG28598.1"
                     /translation="AVIGCNFDPSHLFWQGADPVAAIRAIGDGVFHVHAKDCKLDAAN
                     ISVNGVLDAKPYTKESERSWIFRTVGYGNGAPVWKAIVSALRLVGYDHVLSIEHEDSL
                     MSGTEGLKKAIAFLKDVVITEPAGEAYWA"
     gene            complement(516..908)
                     /locus_tag="PLT35_04910"
     CDS             complement(516..908)
                     /locus_tag="PLT35_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG28599.1"
                     /translation="MADLEGLKKKYGPVIEYGQKRGVSWKNIHIENNKLLIRGAAPAD
                     FVKNEVWIKIKDIDPLYEDLTADITIDSSLKVPDTMYTVVAGDSLSKIAKRYYGDANQ
                     YMKIFEANKDQLKDPNLIKVGQTLRIPD"
     gene            complement(1004..1879)
                     /locus_tag="PLT35_04915"
     CDS             complement(1004..1879)
                     /locus_tag="PLT35_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOG28600.1"
                     /translation="MDNRTRSLTRRSFLELAGAAPLALAAARSITLPAAARIPVALQL
                     YSVRGDCAKDFDAALAAVAAMGFEGVEFAGYYAYEKDAPALAARLKALNLKAAGTHIR
                     TSSLVGDALKAAIDFHQAIGCRFLIVPSDQAFTDPEKSKALADTFNQAAAVLKPLGMA
                     CGYHNHVNEFKKDGDKTFWDLFAERTTKDVILQQDCGWSMAAGQDPAAYIRKYPGRSR
                     TVHFKPTVREGDAGRKAIFGQDSVDWRSVYDACRSVGGTEWIVLEQEVYPDGRTPMDA
                     TRESFEGVRKLLASA"
     gene            complement(1937..3013)
                     /locus_tag="PLT35_04920"
     CDS             complement(1937..3013)
                     /locus_tag="PLT35_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOG28601.1"
                     /translation="MLTRRELLTSSSLAVLASPASRRRAGAALPGPPGALPSAPPAPK
                     LVKPPRLKAGGTVGLVLPSFVQWDPVSIDILLDSLQALGLKGKLGKHVFDRRGYFAGR
                     DEDRAADLNAMFADPEVDAIHCIRGGWGAARLLPLLDWDAIARHPKAFIGYSDITALL
                     LALHARTGLVTFHGPLGSSSWNAFNVEWMKRVLWNGEAVTFANRKEADDTLVPVRNRT
                     RTITPGRGRGRLLGGNLTVLTAIIGSGYLPDFRNAVLFLEDVEEAPYSVDRMLTQLKL
                     AGILDGVKGVAWGTCEKCDPGQGFGSLTIPDVLDDHLKPLGVPVFSGAMIGHVDRQFT
                     LPLGVDVEIDAGAGTITMLEGAVS"
     gene            3209..4531
                     /locus_tag="PLT35_04925"
     CDS             3209..4531
                     /locus_tag="PLT35_04925"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG28602.1"
                     /translation="MIHSRGCSRRTFLRTVPAAVAGTVAFPTIVRASALGRGGAVAPS
                     ERIVMAGIGFGMQGPSNMRNFLGKPEVQWVACCDLDDAHLQRAKDIVDTSYGNTDCAT
                     YRDYRALYARGDLDAVSIAVPDHWHAILSLAAVRAGLDVYGEKPFTHSLREGRALCSA
                     IERYGRVWQTGSWQRSENNFHRAAELVAAGRIGRIERVEVGLPSGYTDFARTFGQEKI
                     ENPPPGLDYETWLGPAPWWPYCVARVHMNWRWNVDFGGGQLIDWIGHHLDIAHWALGL
                     ERTGPIEVKGTGEFPTSGIYNAARRFWVETKYADGPPMIIAGGYPQIQSGTKWFGEHG
                     WVWVDRDGFESQPAHLVNEVIGPNEKRLYKSRDHAQNFLDCVRSRALTIAPAEVAHRS
                     ASVGHLGQIAIETGRTIRWDPAAEAIIGDPGAERMLARGYRAPYQLPA"
     gene            4545..6557
                     /locus_tag="PLT35_04930"
     CDS             4545..6557
                     /locus_tag="PLT35_04930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG28603.1"
                     /translation="MTTITLRRFAPGLAAARAVFLASALLASASAAVTAQPAAAPSSL
                     DAILKALATHDGGIESAPVWSLRDYVYARKDDPAGRAECEAALLAFLKTPATPPAKLA
                     AARYLRVVAGDTAVPALRALLLADARTADCAIAVLQRLPGPAADDALAGALKPARGAV
                     KTALVAALGERRSAAALPALVPLLAQPALAEAAATAIGRIGGAGAVSALMPELTRAAG
                     PFKQAVAAALLAAADQMRASGDFAGALPAFRRLASDTSLPSPLRTAAFMGEIGAGEEG
                     AAGLLLTMLDSADPDERQAAVAKIADAVPAEGIGPVCDRLRKLPEALQVQVLAALTGY
                     PRVRVLGTVLWAARSESPEVRVAALRALAQVGDASIVPFLVERAASAPRGPEQDAARS
                     ALAAIRGRDVDDAVVGLLDRKPAPAAAVELLKAIGERRVFTAKPAVAAALADEPSAVR
                     IEALKALRAIGSPSDVSPVLDLLVGTRDDSEQAEAEKTVAALLQKIGAPDGRSRLLRA
                     RLLATQEPAERARLLALLPLVGDDGSLPVLRKALGDSDGEVAGAAARAIAAWPTVTAR
                     DDVLRLARGAKDDTHTLLALAGLVRLIGLEPNRLPEAAVADLRQAADLASRPEERKLV
                     LGVLAAFPCEAAFEFARGFLQDEAVKAEAQAAIDAMTQRLAKGPAR"
     gene            6574..7245
                     /locus_tag="PLT35_04935"
     CDS             6574..7245
                     /locus_tag="PLT35_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOG28604.1"
                     /translation="MTKKTVVLTFLCAFLLAPLAAEQAGKMEELKLKLPKPMFVGTPT
                     NIKSANLEVVTGKSRGPFMVPAGTKLLSAKKPVRGSDMQPVIGELDMITDGEKEGGDG
                     YFVELGPGKQWVQIDLGASYALHAILVWHYHSQARVYRDVVVQVSADKDFLKGVQTIF
                     NNDHDNSSGLGVGKDKEYIEVAEGRLIDPKGVQGRYVRLYSNGNTTNDLNHYVEVEVF
                     GTPGA"
BASE COUNT         1061 a         2576 c         2574 g         1060 t
ORIGIN      
        1 cggcggtcat cggatgcaac ttcgacccgt cgcacctgtt ctggcagggg gcggacccgg
       61 tggccgccat ccgggccatc ggcgacggcg tgttccacgt ccacgcgaag gactgcaagc
      121 tcgacgcggc caacatcagc gtgaacggcg tgctcgacgc caagccgtac acgaaggaaa
      181 gcgagcgctc gtggatcttc cggacggtgg gctacggcaa cggggcgccc gtgtggaagg
      241 cgatcgtcag cgccctgcgc ctcgtcggct acgatcacgt gctcagcatc gagcacgagg
      301 acagcctgat gtccggcacc gaaggcctga agaaggcgat cgcgttcctg aaggacgtgg
      361 tgatcacgga gccggcgggg gaggcgtact gggcgtaggg gttcgggact cggggctcgg
      421 gacccggggc tcggaacgcg cgcggaaggg ggcggcggca cgaagccggc gccccctttt
      481 tctgccgcgg ccgcgatgcg gcgaatcgcg ccgcgctagt ccggaatcct cagcgtctgg
      541 cccaccttga tcaggttcgg atccttcagc tggtccttgt tcgcttcgaa gatcttcatg
      601 tactggttcg cgtcgccgta gtaccgcttc gcgatcttcg acagggagtc gcccgccacc
      661 acggtgtaca tcgtgtccgg aaccttcagc gacgagtcga tcgtgatgtc ggccgtcagg
      721 tcctcgtaga gcggatcgat gtccttgatc ttgatccaca cttcgttctt cacgaagtcg
      781 gccggagcgg cgccgcggat gagcagcttg ttgttctcga tgtggatgtt cttccagctg
      841 acgccgcgct tctgcccgta ctcgatcacc gggccgtact tcttcttgag cccttccaga
      901 tcagccatga cacgtcccct ttcctcgacc tcggaggcca tcccggcggg ttagcgaagt
      961 acagcaacgc cggcgcgccc gccggacaac ccctcccgcc tccctacgcc gacgcgagca
     1021 gcttcctgac gccctcgaac gactcccgcg tggcgtccat cggcgtcctg ccgtcaggat
     1081 agacctcctg ctccaggacg atccactccg ttccgccgac cgaccggcag gcgtcgtaca
     1141 ccgacctcca gtccaccgag tcctgtccga agatcgcctt cctgccggcg tcgccctcgc
     1201 gcaccgtcgg cttgaagtgg acggtccggc tccggccggg gtacttgcgg atgtacgccg
     1261 ccgggtcctg ccccgccgcc atcgaccacc cgcagtcctg ctgcaggatc acgtccttcg
     1321 tcgtccgctc ggcgaagaga tcccagaagg tcttgtcgcc gtccttcttg aactcgttca
     1381 cgtggttgtg gtacccgcac gccatcccca ggggtttcag gacggccgcg gcctggttga
     1441 acgtgtccgc gagcgccttg ctcttctcgg ggtcggtgaa cgcctggtcg cttggcacga
     1501 tgaggaagcg gcacccgatc gcctggtgga agtcgattgc ggccttcagg gcgtcaccca
     1561 cgaggctgct ggtgcggatg tgcgtgccgg ccgccttgag gttcagcgcc ttgagccgcg
     1621 cggccagggc cggcgcgtct ttctcgtacg cgtagtagcc ggcgaactcg acgccctcga
     1681 agcccatcgc cgcgacggcg gcgagggcgg cgtcgaagtc cttcgcgcag tcgccgcgca
     1741 ccgagtacag ctgcagggcc acggggatcc gcgcggcggc cggcaacgtg atcgaccgcg
     1801 cggcggccag cgcgagcggc gcggcgccgg ccagttccag gaacgagcgc cgggtcaggg
     1861 aacgcgttcg gttgtccatg tcaggctccg tgaagaacgc caaccggttc gacggggatt
     1921 atggcacgaa ccgccgtcac gacacggcgc cctcgagcat cgtgatcgtg ccggcgccgg
     1981 cgtcgatctc gacgtccacg ccgagcggga gcgtgaactg acggtccaca tggccgatca
     2041 tcgcgcccga gaacaccggc acgccgagcg gcttcaggtg gtcgtccagc acgtcgggaa
     2101 tcgtcaggga gccgaagccc tgccccggat cgcacttctc gcacgtgccc cacgccacgc
     2161 ccttcacgcc gtccaggatg cccgccagct tgagctgcgt cagcatccgg tccacgctgt
     2221 agggcgcctc ttcgacgtcc tccaggaaca ggacggcgtt cctgaagtcc ggcaggtagc
     2281 cggagccgat gatggcggtc agcaccgtga ggttgccgcc gaggaggcgg ccccgccccc
     2341 ggccgggcgt gatcgtgcgc gtgcggttcc tgaccggcac cagcgtgtcg tcggcttcct
     2401 tccggttcgc gaaggtgacc gcctcgccgt tccagagcac gcgcttcatc cactccacgt
     2461 tgaaggcgtt ccacgacgac gacccgagcg ggccgtgaaa ggtcaccagg ccggtcctgg
     2521 cgtgcagcgc cagcagcagg gccgtgatgt cgctgtagcc gatgaacgcc ttgggatgcc
     2581 tggcgatggc gtcccagtcg aggagcggca gcagccgcgc cgctccccag ccgccccgga
     2641 tgcagtggat ggcgtcgacc tccgggtcgg cgaacatcgc gttcaggtcg gccgcgcggt
     2701 cttcgtcgcg gccggcgaag tagccgcgcc gatcgaagac gtgcttcccg agcttgccct
     2761 tgaggccaag cgcctgcagc gagtccagga ggatgtcgat cgacaccggg tcccactgca
     2821 cgaacgacgg aaggacgagg cccaccgtgc cgcccgcttt cagccggggc ggcttgacga
     2881 gcttcggagc cggcggggcc gaaggcaggg cgccaggcgg accaggcagg gcggcgcccg
     2941 cccgcctgcg ggaggccggc gacgccagca cggccagcga cgaggatgtc agcagttcgc
     3001 ggcgtgtgag catgccgggc atgctacgcc caggcccgga cgaatgcaaa ggagccggcc
     3061 gggccagcgt cgccgcggcg gccgctggag cgtcgccgtc ggcccgtcca gccgcgttgg
     3121 tcaggcgccc ggtgcctgcg gtagaatcgc ggccgtcgtt gccgggcccc ttccccgggg
     3181 ctcctccgtg acgcgggaga acacgacgat gatccactcg cgcggatgca gccgccgcac
     3241 gtttctcaga accgtcccgg ccgccgtcgc cgggaccgtc gcgttcccca ccatcgtccg
     3301 ggcgtcggcg ctcggccgcg gcggcgcggt ggcgccgagc gagcgcatcg tcatggccgg
     3361 catcgggttc gggatgcagg gcccgtccaa tatgcgcaac ttcctcggca agcccgaggt
     3421 gcagtgggtc gcctgctgcg acctcgacga tgcccatctc cagcgggcga aggacatcgt
     3481 cgacaccagc tacggcaaca cggactgcgc gacctacagg gactaccgcg cgctctacgc
     3541 gcgcggcgat ctcgacgccg tgtcgatcgc cgtgcccgac cactggcacg cgatcctgtc
     3601 actggcggcc gtgcgggcgg gcctcgacgt ctacggggag aagccgttca cgcacagcct
     3661 ccgggaaggg cgggccctgt gcagcgccat cgagcgctac ggccgcgtgt ggcagacggg
     3721 gagctggcag cgttccgaga acaacttcca ccgggcggcg gagctggttg ccgcgggccg
     3781 gattggccgg atcgagcgcg tggaagtcgg cctgccatcc ggctacaccg acttcgcgcg
     3841 cacgttcggc caggagaaga tcgagaaccc gccgcccggg ctggactacg agacgtggct
     3901 cgggccggcg ccgtggtggc cgtactgcgt ggcgcgcgtg cacatgaact ggcgctggaa
     3961 cgtggacttc ggcggcggcc agctcatcga ctggatcggt caccatctgg acatcgccca
     4021 ctgggcgctc ggcctcgagc gcaccgggcc gatcgaggtg aaggggacgg gcgagttccc
     4081 cacgagcggc atctacaacg ccgcgcgccg cttctgggtc gagacgaagt acgccgacgg
     4141 cccgcccatg atcatcgccg gcggctaccc gcagatccag tccggcacga agtggttcgg
     4201 cgagcacggc tgggtgtggg tggatcgcga cgggttcgag agccagccgg cccacctggt
     4261 caacgaggtc atcggcccca acgagaagcg cctctacaag agccgcgacc acgcgcagaa
     4321 cttcctcgac tgcgtgcgca gccgcgcgct cacgatcgct ccggccgagg tggcgcaccg
     4381 gtcggcgagc gtcgggcacc tcggtcagat cgccatcgag accgggcgga ccatcaggtg
     4441 ggacccggcc gccgaggcga tcatcggcga tcccggcgca gagcggatgc tcgcccgcgg
     4501 ctatcgcgcg ccctaccagc ttccggcgtg agacgaggtg ccctgtgacg accatcacgc
     4561 tccgccgctt cgcgccgggc ctcgcggcgg cccgagccgt cttcctggcg tccgccctgc
     4621 tggcgtccgc gtccgcggcc gtgacggccc agccggccgc cgccccctcg tctctcgatg
     4681 cgattctcaa ggcgctcgcc acgcacgacg gcgggatcga gtcggccccg gtgtggagcc
     4741 tccgcgatta cgtgtacgcg cgcaaggacg atccggccgg ccgcgccgaa tgcgaggcgg
     4801 cgctgctggc gttcctcaag accccggcca cgccgccggc aaagctcgcc gccgcgcggt
     4861 acctgcgcgt ggtggcgggc gacacggccg ttccggcgct ccgcgcgctg ctgctcgccg
     4921 acgcgagaac ggccgactgc gccatcgccg tcctgcagcg cctgccgggc ccggccgcgg
     4981 atgatgcgct cgccggcgcg ctgaagccgg cccgtggcgc cgtgaagacg gccctcgtgg
     5041 cggccctggg cgagcggcgt tcggcggcgg cgctgcccgc gctcgttccc ctgctggcgc
     5101 agcccgcgct cgcagaggcg gcagccacgg ccatcgggag aatcggcggc gcaggcgcgg
     5161 tgtcggcgct gatgccggag ctgacccgcg ccgccggtcc gttcaagcag gccgtcgccg
     5221 ccgcgctgct ggcggcggcc gaccagatgc gggcctccgg ggacttcgcc ggcgcgctgc
     5281 cggccttccg ccggctggcc tccgacacgt ccctgccgtc tcccctccgc acggcggcgt
     5341 tcatggggga gataggcgcc ggggaggaag gcgccgcggg ccttctgctc accatgctcg
     5401 actcggccga ccccgacgag cggcaggcgg cggtcgcgaa gatcgcggac gcggttcctg
     5461 ccgaagggat cgggcccgtc tgcgatcgcc tgcggaagct gccggaggcg ctccaggtgc
     5521 aggtgctggc cgcgctgacc ggctatccgc gggttcgcgt gctggggacg gtgttgtggg
     5581 ccgcccgcag cgagtcgccg gaggtccgcg tcgcggcgct gcgcgcgctg gcgcaggtcg
     5641 gcgacgcatc gatcgtcccg tttctcgtcg agcgcgccgc gtcggcgcca cgcgggcccg
     5701 agcaggacgc cgcacgcagc gccctggccg cgatcagggg ccgggacgtc gatgacgcgg
     5761 tcgtcgggct gctcgaccgc aagccggcgc cggccgcggc cgtcgagctg ctcaaggcca
     5821 tcggcgagcg gcgcgtgttc accgcgaagc cggccgtcgc cgccgccctg gcggacgagc
     5881 cgtcagccgt tcgcatcgag gcgctgaagg cgcttcgcgc catcggatcg ccttcggacg
     5941 tgtcgcccgt gctcgatctc ctcgtcggca cccgcgacga ctccgagcag gcggaggccg
     6001 agaagaccgt ggccgccctg ctgcagaaga tcggtgcgcc ggacggacgg tcgcggctcc
     6061 tccgggcccg tctcctggcg acgcaggaac ccgccgagcg cgcgcggctg ctggccctcc
     6121 tgccgctcgt cggcgatgac gggtcgctgc ccgtcctgcg gaaggcgctc ggcgactcgg
     6181 acggcgaagt cgccggcgcc gccgcgcggg cgatcgccgc ctggcccacg gtgacggccc
     6241 gggacgacgt cctccgcctg gcccgcggcg cgaaggacga cacgcacacg ctgctggcgc
     6301 tggccggcct ggtccgcctg atcggcctgg agccgaaccg gctccccgag gcggccgtcg
     6361 ccgacctcag gcaggcggcg gacttggcaa gccggccgga agagcggaaa ctggtattag
     6421 gcgtcctcgc ggcgtttccc tgcgaggccg cgttcgagtt cgcgagaggg ttcttgcagg
     6481 acgaagcggt gaaggccgag gcccaggcgg ccatcgacgc catgacgcag cgcctggcca
     6541 agggccccgc ccgctgaaag ggtgagaaga gacgtgacca agaagactgt cgtgctgacg
     6601 ttcctgtgcg ccttcctcct cgcgcccctg gccgccgagc aggcgggcaa gatggaagag
     6661 ctgaagctga agctgccgaa gccgatgttc gtcggcacgc caaccaacat caagagcgcg
     6721 aacctggagg tcgtgaccgg caagtcgcgc gggccgttca tggtccccgc cgggacgaag
     6781 ctgctgtcgg cgaagaagcc cgtccggggc agcgacatgc agccggtcat cggcgagctc
     6841 gacatgatca ccgacggcga gaaggaaggc ggagacggct acttcgtcga gctcggaccc
     6901 ggcaagcagt gggtccagat cgatctcggc gcgtcgtacg cgctgcacgc gatcctcgtc
     6961 tggcactacc actcgcaggc gcgcgtctat cgcgacgtcg tcgtgcaggt gtccgccgac
     7021 aaggacttcc tgaagggcgt gcagacgatc ttcaacaacg accacgacaa ctcgtccggg
     7081 ctcggcgtcg gcaaggacaa ggagtacatc gaggtcgccg aggggcggct catcgacccg
     7141 aagggggtcc agggccgcta cgtgcgcctt tactcgaacg gcaacaccac gaacgacctg
     7201 aaccactacg tcgaggtcga ggtcttcggc acgccgggcg catgagcaag gccggcttcg
     7261 gcggcgcggt c
//
LOCUS       DAOSIF010000101         7118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9587_length_7118_cov_4.299731, whole genome shotgun sequence.
ACCESSION   DAOSIF010000101 DAOSIF010000000
VERSION     DAOSIF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9587_length_7118_cov_4.299731"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1251)
                     /locus_tag="PLT35_04940"
     CDS             complement(112..1251)
                     /locus_tag="PLT35_04940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOG28605.1"
                     /translation="MSQVTMETETPRPMFDTDEMVLNMGPQHPSTHGVLRLVLKLDGE
                     RVVGCDPVIGYLHRGIEKLSEHRDYTQVVLLTDRMDYVAAATNNLGYVETIEKLYSLE
                     VPRRARYIRTILAELQRVASHLLWLGSSAGDLGALTVLLFGLRERELVLDLFEEYCGA
                     RLTYNTMRIGGQPVDVPPDWGRKILAFCDEQERKLPDYEDLLTNNRIFLQRTKGIGVI
                     SGADAIALGLSGPPLRGSGVARDVRKDEPYAAYDEMDFDIAVGTEGDTYDRYLVRLEE
                     IRQSLRILRQAVDGLPEGPVMGKVPRLIKPPAGETYHAIEAPKGELGFFVASDGKSVS
                     PYRMRVRPASFCNLQALPRLVVGHLVADVVALVGTIDIVLGEVDR"
     gene            complement(1248..1943)
                     /locus_tag="PLT35_04945"
     CDS             complement(1248..1943)
                     /locus_tag="PLT35_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOG28606.1"
                     /translation="MSEGEKPAPEQIPTAPPAAKPAAPSGPKGEAKAGPAKTEAKPAA
                     PPGPPDPEPPPDAAVPACVTALQSGLPGAVEHVSVWVGDWTVVVPAGRLVDAAAFLRD
                     QAACRFDYLSDLTAVDWLGRDARFDVVLCLYSTALRHRLRVKVRVADGEPVPSVAGLW
                     PAANWLEREVFDMFGITFTGHPDLRRILMPPDWQGHPQRKDYPLEGPGELLMENPQDW
                     LKARQAAVESGIE"
     gene            complement(1957..2313)
                     /gene="ndhC"
                     /locus_tag="PLT35_04950"
     CDS             complement(1957..2313)
                     /gene="ndhC"
                     /locus_tag="PLT35_04950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOG28607.1"
                     /translation="MVTTWIPILIFIAVAVAFGIVVLLLAWSVAPQRYSKAKLQPYEC
                     GIEPLTDARERFNVRYFLVAMLFLIFDVETIFLYPWAVIAEELAWFGFVEILVFISIL
                     VAGYFYAWREGALEWV"
     gene            2661..3578
                     /locus_tag="PLT35_04955"
     CDS             2661..3578
                     /locus_tag="PLT35_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28608.1"
                     /translation="MHLRLTHPPASPALLALLGALAMAASASTSAAQPAERDMFVSVV
                     DRGGSPVLTLGPEDFVVRDDDRLREVLRARRASDLLHIAVLVDTSQALGSQVSDVRQG
                     LLAFAARMGAQARIALVGFGDRPTVLSDYSSSADAVARGVGLIFPIQGAGACVLDAVD
                     ETLAGLEKAGPERSAIVVVWAGGREFGHATDTELIERLAANGTALHVLVIGGGVPEDV
                     RTPEGRHREGLFDRGTTATGGRRTNVLSSMAAADALERLAAELLGQYRITFARPESLI
                     PPKKTIVAVRPEGLTARGLLVPVRAAARQ"
     gene            3599..4528
                     /locus_tag="PLT35_04960"
     CDS             3599..4528
                     /locus_tag="PLT35_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG28609.1"
                     /translation="MAAALAFACHPAAALGQSVPAEAPPAAQAQAYRSGIDLVSLTVT
                     VTDGSNRYVTDLDSGDFTVFEDGVRQDVGVFSRTSLSLSVALLLDSSASMEERMSVAR
                     TAALGFVASLRPQDEVQLIDFDSRVRVVAPFTTSRPDLERAVRSVEAGGSTALYTAIY
                     VALREMAKTEARSVEAQRRQAIIVLSDGEDTTSLVSYDAVLDLARRSDTVIYAIGIDA
                     RSAGRGKGFNEAAFVLRELTAQTGGQAFFPERLDELPAIYAHISRELSSQYLLGYVSS
                     NSRRDGEWRRVSVRVSRPGAAARTRSGYYAPAR"
     gene            4602..6158
                     /gene="cobA"
                     /locus_tag="PLT35_04965"
     CDS             4602..6158
                     /gene="cobA"
                     /locus_tag="PLT35_04965"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOG28610.1"
                     /translation="MPNRPRVCFIGAGPGDPGLLTLRGARCLGRADVVVYDSLVHAAL
                     LRHAPPGAERIDVGRAAPQGTDRDAIAYLLVEKVREGKHVARLKWGDPFIFDDGGEEA
                     LFLHEHGIPFEVVPGIPAAVGVPPYAGVAVTYRGGGDTLTLVRGFEDARGRLPRIDWA
                     SLARLDGTIVCYAGPAQAPAILAALAAHGRPADEPAALIYSGTLATQQTVAGTLSSLA
                     GAPPPADSRPAIVVVGRVAALREHLRWFDVRPLFGRRIVVTRPREQARELVDLLEDQG
                     AQVVLAPTVRLAPAGDGSELDEVCAGIGAFDWVVLPTLTGAEVFLRRLTTAPHDIRDL
                     KGVGLCAIGQSSAERLAAFGLRADIAPPELRPDALVEALAARKGVRGRQVLLVQAEGA
                     RDLLAAELRKAGAEVREVGAYRIERVLPGDPGEPDLYKMLLEQQIDVVTFVSPSTVRE
                     FVDIHGADAVADVLRTTLVACVGPVTAQAAIAHGLMPAIVPEEFTMAAMVQAIVRYLR
                     NPPDAASSPR"
     gene            complement(6142..>7118)
                     /gene="rseP"
                     /locus_tag="PLT35_04970"
     CDS             complement(6142..>7118)
                     /gene="rseP"
                     /locus_tag="PLT35_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOG28611.1"
                     /translation="PPSLEPEEVADAPVDVAVVTKGSPAERGGILPGDRIVRVGSQKT
                     PTWERFYLAIGGRAERDVRVVLLREGRELTVTVRPEARTKMQVGDIGVLPKVHPGIRS
                     VVAGGPADRAGIKAGDVVLALNGEPIIFSGQLSAEIRKHPEEAITLTLERDGAPLDVR
                     VIPRRQGQIGLIGIGIQDPYRTIKPGPLGSLTLSLKRNVEYSGLIFRTIGGLLTRETS
                     ANQLMGPVAIAQMSGDYAAAGWIALFTFMASLSLNLGLLNLLPIPVLDGGHIFIMALE
                     GVARRDFSMKVKERLMLAGFLVLMALMVVVVYNDLARLSLFDRFSAGN"
BASE COUNT         1011 a         2607 c         2480 g         1020 t
ORIGIN      
        1 cgaacagcac ggtgaggatc acgatcagcg ggacgatgac ggtttctacc atgtcagcgt
       61 gtcttgtccg cgtcctcgag ccccgagtcc cgggccccgg gtccccactt cctaccgatc
      121 cacttccccg agcacgatgt cgatggtgcc gacaagcgcc acgacgtccg ccaccaggtg
      181 cccgaccacg aggcgcggca gcgcctggag gttgcagaag gaggccggcc gcacgcgcat
      241 ccggtacggc gacaccgact tgccgtcgct cgccacgaaa aaccccagct cgcccttcgg
      301 cgcctcgatc gcgtggtacg tctcgccggc cggcggcttg atgagccgcg gcaccttgcc
      361 catgaccggg ccctccggca ggccgtccac cgcctgccgc aggatgcgca gggactgccg
      421 gatctcctcc aggcgcacga ggtaccggtc gtaggtgtcg ccctcggtgc ccaccgcgat
      481 atcgaagtcc atctcgtcgt acgcggcgta gggctcgtcc ttgcgcacgt cccgcgccac
      541 gcccgacccc cgcagcggcg gcccggacag ccccagcgcg atggcgtccg cgccggagat
      601 gaccccgatg cccttggtcc gctgcaggaa gatgcggttg ttcgtcagca ggtcctcgta
      661 gtccggcagc ttgcgctcct gctcgtcgca aaacgccagg atcttcctgc cccagtccgg
      721 cgggacgtcc accggctgcc cgccgatccg catcgtgttg tacgtcaggc gtgcgccgca
      781 gtactcctcg aacaggtcca ggaccagttc ccgctcgcgc aggccgaaca ggagcaccgt
      841 cagggccccg agatcgccgg cgctcgatcc gagccagagc aggtggctcg ccacccgctg
      901 cagctccgcg agaatcgtgc ggatgtagcg cgcccgccgc ggcacctcga gggagtacag
      961 cttctcgatc gtctcgacgt atcccaggtt gttcgtcgcg gctgcgacgt agtccatccg
     1021 gtccgtcagc aggacgacct gcgtgtagtc ccggtgctcg ctcagcttct cgatcccgcg
     1081 gtgcaggtag ccgatgaccg gatcgcagcc gacgacgcgc tcgccgtcca gcttcagcac
     1141 gaggcggagc accccgtgcg tgctggggtg ctggggcccc atgttaagga ccatctcgtc
     1201 ggtgtcgaac atcgggcggg gcgtctcggt ctccatggtc acctggctca ctcgatgccg
     1261 gactcgacgg cggcctgacg cgccttcagc cagtcctgcg ggttctccat cagcagttcg
     1321 cctggcccct cgagcggata gtccttccgc tgcggatgcc cctgccagtc cggcggcatc
     1381 aggatccgcc gcaggtccgg gtggcccgtg aacgtgatgc cgaacatgtc gaagacctcg
     1441 cgctcgagcc agttcgccgc cggccacagg ccggcgacgc tcggcacggg ctccccgtcg
     1501 gccacccgca ccttcacgcg cagccggtgg cggagcgccg tcgagtagag gcacagcacg
     1561 acgtcgaagc gggcatctcg gcccagccag tccaccgccg tcaggtcgga caagtagtcg
     1621 aaccggcacg ccgcctggtc gcggagaaag gccgccgcgt cgacgaggcg gcccgccggc
     1681 acgacgacgg tccagtcccc cacccagacg ctcacgtgct cgacggcgcc cggcagcccc
     1741 gactgcaggg ccgtcacgca cgccggcacg gcggcatcgg gcgggggttc cggatcgggc
     1801 ggcccgggcg gcgccgcggg cttggcctcg gtcttggccg ggccagcctt cgcctcgcct
     1861 ttcggccccg agggcgcggc cggcttcgcc gccggcggcg cggtcgggat ctgttccggt
     1921 gccggcttct cgccttctga catcagcggc ctccgcctag acccactcca gggcgccttc
     1981 acgccaggca tagaagtacc cggcgacgag gatcgagatg aacacgagga tctcgacgaa
     2041 cccgaaccag gccagctcct ccgcgatgac ggcccacggg tagagaaaga tcgtctccac
     2101 gtcgaagatc agaaacagca tcgccaccag gaagtaccgc acgttgaagc gctcgcgcgc
     2161 gtcggtcagc ggctcgatgc cgcactcgta gggctggagc ttggccttcg agtaccgctg
     2221 cggcgcaacc gaccacgcca ggagcagcac gacaatgcca aacgccaccg ccaccgcgat
     2281 gaagatcagg atgggaatcc aggtcgtgac catcgtgcga ctcacctgcc cgcgcccgcg
     2341 ggcgcgctcc tctcccggcc gaagtccctg gacggacgcg tgagggcgcc cagttgtctg
     2401 acggtatgca cggctgctcg tgtggacggc gcgacggcat ctcccggcgg cgcgcgcgtc
     2461 gcgaccggcc ggcaggctcc gaacccccgc cgaaaaccgg ccgcacgccc gctgacccag
     2521 cggccgcgcc cggcagaacg gctttcgggt tataacacgc ggtccgaagg cgggtcaagg
     2581 taggcaagtg ccgtgctgac aagcgtttcg cggaaacgct atactggcct catccctgcc
     2641 tgaaggaagg acgaaccgcc gtgcatctcc gcctcacgca cccgccggcc tcgcccgcgc
     2701 tcctcgcgct cctcggcgcg ctggcgatgg cagcctccgc ctcgacctcc gccgcccagc
     2761 cagccgagcg ggacatgttc gtgagcgtcg tcgaccgcgg cggctccccc gtcctgacgc
     2821 tcggccccga ggatttcgtc gtgcgcgacg acgaccgcct ccgcgaagtg ctccgcgcga
     2881 ggcgggcttc cgatctcctc cacatcgccg tgctcgtgga cacgagccag gcgctcggca
     2941 gccaggtctc cgacgtccgg cagggcctgc tggcgttcgc cgcccgcatg ggcgcgcagg
     3001 cgcgcatcgc cctggtcggc ttcggcgacc ggccgaccgt gctcagcgac tactcgtcga
     3061 gcgcggacgc cgtggcgcgc ggcgtgggct tgatcttccc gattcaagga gctggcgcgt
     3121 gcgtcctcga cgccgtggac gagacgctcg cgggtctgga gaaggccggg ccggaacggt
     3181 ccgccatcgt ggtcgtgtgg gcgggcgggc gcgagttcgg acacgccacg gacacggagc
     3241 tcatcgagcg cctcgcggcc aacggcacgg ccctgcacgt gctcgtcatc ggcggcggcg
     3301 tgccggagga cgtccggacg cccgagggcc gccaccgcga agggctcttc gaccgcggca
     3361 ccacggccac tggcggccgc cgtacgaacg tgctgtcgtc gatggcggcc gccgacgcgc
     3421 tggagcgcct cgcggcggag ctcctgggcc agtaccgaat cacgttcgcc aggccggagt
     3481 cgttgatccc tccgaagaag accatcgtcg ccgtgaggcc ggaggggctg acggcgcggg
     3541 gcctcctggt ccccgtccgg gccgccgcca ggcagtgagg aggagcagcc tcgcctcgat
     3601 ggcggcggcg ctggccttcg catgccaccc cgccgcggcc ctcggacaat cggttcccgc
     3661 cgaagcgcct ccggcagccc aggcccaggc gtaccgcagc gggatcgacc tcgtctcgct
     3721 gacggtcacc gtcaccgacg gctcgaaccg gtacgtcacg gacctcgaca gcggcgactt
     3781 caccgtcttc gaggatggcg tccgacagga cgtcggcgtc ttcagccgca cgagcctctc
     3841 gctctcggtc gccctgctgc tcgacagcag cgccagcatg gaggaacgaa tgagcgtcgc
     3901 ccggacggcc gcgctcgggt tcgtggcgag ccttcgcccg caggatgagg tgcagctcat
     3961 cgacttcgac agccgagtcc gggtggtcgc gcctttcaca acctcgcggc cggatctcga
     4021 gcgggcggtg cgttcggtcg aggcgggagg ctcgacggcc ctctatacgg cgatctacgt
     4081 ggcgctgcgc gagatggcga agacggaagc ccggagcgtc gaggcgcagc gccgccaggc
     4141 gatcatcgtg ctctcggacg gggaagacac cacgagcctc gtgtcctacg acgcggtgct
     4201 cgacctggcc aggcgctccg acactgtcat ctacgccatc ggcatcgatg cccggtccgc
     4261 cgggcggggc aagggcttca acgaggcggc cttcgtcttg cgggagctga cggcgcagac
     4321 ggggggccag gcgttctttc ccgagcggct cgacgagttg cccgcgatct acgcgcacat
     4381 ctcgcgcgag ctgtcgagcc agtacctgct cggctacgtg tcgtccaact ccaggcgtga
     4441 cggagagtgg cggcgcgtga gcgtccgcgt gtcgcgccca ggcgcggccg cccggaccag
     4501 gtccggctac tacgcgccgg cgcgctgaac accgccgcgc cgcgcgcccc cgcctttgat
     4561 atgcttgggc cgctcacggg tcctgccgag cgaggtgcgc gatgccgaac cgcccgaggg
     4621 tgtgcttcat cggcgccggg ccgggtgacc ctggcctcct gacgctgcgc ggggcgcggt
     4681 gtctcgggcg cgccgacgtg gtggtgtacg acagcctcgt ccacgccgcg ctgctgcgcc
     4741 acgcccctcc cggggcggaa cgcatcgacg tgggccgcgc ggcgccgcag ggaaccgacc
     4801 gcgacgccat cgcctacctg ctggtcgaga aggtccgcga gggcaagcac gtcgcgcggc
     4861 tcaagtgggg cgaccccttc atcttcgacg acggcggcga ggaggccctt ttcctgcacg
     4921 agcacggcat cccgttcgaa gtcgtgccgg gcatcccggc cgcggtgggc gtcccgccgt
     4981 acgcgggggt ggcggtgacc taccgcggcg gcggcgacac gctgaccctc gtgcgggggt
     5041 tcgaggacgc gcgcggcagg ctgcccagga tcgactgggc gagcctggcg cgcctcgacg
     5101 ggacgattgt ctgctacgcg gggccggccc aggcgccagc catcctcgcc gcgctggcgg
     5161 cgcacggccg gccagccgac gagccggccg cgctcatcta cagcggcacg ctcgccaccc
     5221 agcagaccgt cgccggcacg ctctcgtccc tggccggcgc gccgcccccg gccgattcgc
     5281 gcccggccat cgtcgtggtg ggccgcgtcg cggccctgcg cgagcacctg cgctggttcg
     5341 acgtgcggcc gctcttcggc aggcgcatcg tcgtaacgcg gccgcgcgag caggcgcggg
     5401 agctggtcga tctgctggag gaccagggcg cgcaggtcgt cctggcgccg accgtacgcc
     5461 tcgcgcccgc gggcgacggc tccgagctcg acgaggtctg cgccggcatc ggcgccttcg
     5521 actgggtcgt cctgccgacg ctcaccgggg ccgaggtgtt cctgaggcgg ctgacaacgg
     5581 cgccgcacga catccgcgac ctgaagggcg tcgggctctg cgccatcggc cagtcctcgg
     5641 ccgaacggct cgcggccttc ggcctgcgcg cggacatcgc gccgcccgaa ctgaggcccg
     5701 acgccctcgt cgaggccctc gccgcccgga agggcgtgcg cggccgccag gtcctgctgg
     5761 tgcaggccga aggcgcccgc gacctgctgg cggcggagct gcgcaaggcc ggcgccgagg
     5821 tgcgggaagt cggggcatac cggatcgagc gcgtcctgcc gggcgatccc ggcgagccgg
     5881 acctctacaa gatgctgctc gagcagcaga tcgacgtcgt caccttcgtc agcccgtcca
     5941 ccgtgcgcga gttcgtggac atccacggcg ccgacgccgt ggccgacgtg ctgaggacga
     6001 cgctcgtggc gtgcgtgggg ccggtgacgg cccaggcggc catcgcccac ggcctcatgc
     6061 ccgcgatcgt gccggaggag ttcacgatgg cggccatggt ccaggccatc gtgcgctatc
     6121 tgagaaatcc gccggacgcc gctagttccc cgcgctgaag cggtcgaaca gactgaggcg
     6181 cgcgaggtcg ttgtagacga ccacgaccat cagcgccatc aggacgagga agcctgccag
     6241 catcaggcgc tccttgacct tcatgctgaa gtcgcggcgc gcgacgcctt cgagggccat
     6301 gatgaagatg tgcccgccgt ccaagacggg gatgggcagc aggttcagca ggccgaggtt
     6361 caggctgagc gacgccatga aggtgaacag ggcgatccag ccggcggcgg cgtagtcgcc
     6421 cgacatctgg gcgatggcca ccgggcccat gagctggttg gccgaggtct cgcgcgtcag
     6481 cagcccgccg atggtccgga agatcaggcc cgagtactcc acgttgcgct tgaggctcag
     6541 cgtgagcgat ccgagcgggc cgggcttgat cgtgcggtac ggatcctgga tgccgatgcc
     6601 gatcaggccg atctggccct ggcgccgcgg aatcaccctc acgtcgagcg gcgcgccgtc
     6661 ccgctcgaga gtcagggtga tggcctcttc ggggtgcttg cggatctcgg ccgagagctg
     6721 gccggagaag atgatcggct cgccgttcag cgcgagcacc acgtcgccgg ccttgatgcc
     6781 ggctctgtcg gcgggccctc cggcgacgac gctgcggatc ccggggtgca ccttcggcag
     6841 cacgccgatg tccccgacct gcatcttcgt ccgcgcctcg ggcctgacgg tgaccgtcag
     6901 ctcgcggccc tcgcgcagca gcacgacccg cacgtcgcgc tcggcgcggc cgccgatggc
     6961 gaggtagaag cgctcccacg tcggggtctt ctggctcccc accctgacga tgcggtcgcc
     7021 cggcaggatg ccgccgcgct ccgccggcga tcccttcgtt acgaccgcga cgtcgaccgg
     7081 ggcgtcggcg acctcctccg gctccaggga gggaggaa
//
LOCUS       DAOSIF010000102         7109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9605_length_7109_cov_4.164304, whole genome shotgun sequence.
ACCESSION   DAOSIF010000102 DAOSIF010000000
VERSION     DAOSIF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9605_length_7109_cov_4.164304"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..562
                     /locus_tag="PLT35_04975"
     CDS             113..562
                     /locus_tag="PLT35_04975"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOG28612.1"
                     /translation="MAWTHDQMAARAAQELHDGYYVNLGIGLPTLVANFVAKDIEVWL
                     QSENGMLGIGPFPTEDQVDADLINAGKQTVTTLPGSSIFGSHESFAMIRGGHIDLPVL
                     GLLPYVTTDADVRRRKRLRLLAATAAALLAAASSGVFWVLRLWRYVA"
     gene            562..1500
                     /locus_tag="PLT35_04980"
     CDS             562..1500
                     /locus_tag="PLT35_04980"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOG28613.1"
                     /translation="MGRIDDALRRSNVDAARGTGAEAPAPAPSPWQVEEQDIRAAAPG
                     AAGPAGTAAPAAEGATRGARREPPKAARFVVSGGIDARAAERLVASADASPLLVEQFR
                     TLGATLLRIQGERPLKSLLVTSPSPGDGKSHVATNLALTLSDSYHRRVLLIDADLRRP
                     TLHQVFRLANASGLCEALRADTDEKVPTARVSENLTLLPAGRPEANPLAGLSSGRMAR
                     IVADAAAAFDWVIVDSPPVVVLADAHLVSETVDGAIVVVRAGVTRFPDLAAAVDTLGQ
                     DRVLGVVLNALDPSEMRSEGYYSYYYGRSGRASSRR"
     gene            1699..2139
                     /locus_tag="PLT35_04985"
     CDS             1699..2139
                     /locus_tag="PLT35_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28614.1"
                     /translation="MPTEDIRRPIRELECPPGEEYDEDTFNYLLGMERARAERASRRV
                     RLLLATLEGESGESAMITSSCAARLFGGLRQLLRDTDIVGWYRQNRVAGAVLCARLDG
                     SDEETSRAIGARVGEGLKRLLPARTARNLRVRVTQQGPRRVPKG"
     gene            2153..3310
                     /locus_tag="PLT35_04990"
     CDS             2153..3310
                     /locus_tag="PLT35_04990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG28615.1"
                     /translation="MTKSILEALREFADWEKCPGPVPAPLPLVQPSPGPQAGIPVLGE
                     SVFKDVLVRERKCADRFESPFAVVLVDRSDDASGADAWVPILGAIAAVKRDADAVGWL
                     DEGKVLGLLLPDVPGEGALKILDRLRHEVSARLGDAALAVLSMRVYAHRAAPAADGFS
                     FPPPDLLIEAFAPEPARPGRDAVKRVLDIAGGLLLLVVFSPIMAAAALAVKLTSAGPV
                     FHRQVRVGRRGEPFTMLKFRTMHANAGHAIHQDYVTWFIKSGGKGRPNSDGLFKLTND
                     PRVTRAGRFLRKASLDELPQFLNVLRGEMSLVGPRPPLPFEVEQYQPWHRRRVLEAKP
                     GITGPWQVQGRSRTSFDDMVRLDLRYARTHTLWTDLKILLATPRAMFKGAA"
     gene            3310..3807
                     /locus_tag="PLT35_04995"
     CDS             3310..3807
                     /locus_tag="PLT35_04995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG28616.1"
                     /translation="MDPYLSIAPDVKLGKGVKLSKFINLYGCEIGDETKIGACVEIQK
                     GVKVGRRCKISSHTFICEGVTIEDNVFVGHGVTFTNDVYPRATTADGELQTEADWKVE
                     PTLVKKGASIGSGATILPRVTIGEHAIVGAGSVVTRNVAPRTIVWGNPARFLRRIPPE
                     SGGHT"
     gene            3804..4814
                     /locus_tag="PLT35_05000"
     CDS             3804..4814
                     /locus_tag="PLT35_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG28617.1"
                     /translation="MSQLRVGVIGYGYWGPNIVRNLYGIEACSVAALCDTNPAALQKA
                     GRVYPALRTTPDPAEVLESPGIDAVAIVTPVWTHYELARTALENGKHVFVEKPFTSTV
                     RQAEELVELADRKNLRIMVDHTFLFTGAVRKIRELIDKGVLGPLFYFDSSRINLGLFQ
                     HDVSVVWDLAPHDLSIMDYVIGTKAEAVVATGGSHFNHQADMAFLTIYFPGNVIAHIN
                     VNWLSPVKVRTTLIGGRDKMLVWNDLEADEKIKVYDKGVDVTSREGVYDLLVSYRSGD
                     VWAPKVDQTEALKAELAYFAECVTTGKKPFNDGEAGLSVVRMLEAADQSLAERGKVVL
                     LP"
     gene            5096..6253
                     /locus_tag="PLT35_05005"
     CDS             5096..6253
                     /locus_tag="PLT35_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28618.1"
                     /translation="MLLPFQLGEAVGNLRHERYPEALRAVEHISSFTLSHFLYYLARP
                     FLPVGVRRHLQRLHWAGWRRIPFPRWPVDASVETIMKQAVRALLEAGTVRELPFIWFW
                     PDGAPGAAMMTHDVEGPAGAEFCGELMDLDERSGIRSSFQVIPDGPRAAKAATRKLVD
                     DLRRRGFEVNVHDLTHDGRLFRNRDRFLRHAGRINARGREFGSRGFRSGAMYRRQEWF
                     SALDISYDMSVPNVAHLEPQAGGCCTVMPFFMGHVLELPLTAAQDYTVFHILGEYSTR
                     LWREQIDAILAEHGLASFIAHPDYLVDPRAREVYAELLGILSGLRAERGVWVALPSEI
                     DGWWRERREMRLAPDGGSWRVEGRGAERARVAFARFRDGCVVYELEPRVAK"
     gene            6261..>7109
                     /locus_tag="PLT35_05010"
     CDS             6261..>7109
                     /locus_tag="PLT35_05010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG28619.1"
                     /translation="MGAAVHVVDPLADPRWDGLLARHPRASVFHTRGWLDTLKRTYGY
                     EPFALTTTADGPLENGLALCRVKTPFARRLVSLPFSDHCDPLVDRPEDVSAMLGFLKA
                     QQERERWRSVELRPRQAAWGLAAEAPAAGGDGARPSWPAESARYVHHALDLARPAEQV
                     FAGFHPSHTQRAVRRAEREGLTYEAGNSEPLLSAFYGLLRLTRRRHGLPPQPRAWFRN
                     LAASLGDQLAVHLASKDGRPIAAILTLGFKRTVVYKYGASDAAFHRLGAMPFLFSSMT
                     IRAVSRA"
BASE COUNT         1009 a         2596 c         2456 g         1048 t
ORIGIN      
        1 agcccgacgc catccatctg ccgggcatct acgtcgatcg catcgtgctc aacgcacatc
       61 ccgaaaagcg catcgaaaga cgcaccatca ccgagaaagc gggagcctga ccatggcctg
      121 gacgcacgat caaatggcgg cccgtgccgc gcaggaactg cacgacggct actacgtcaa
      181 cctgggcatc ggcttgccca cgctggtggc caacttcgtg gccaaggaca tcgaggtctg
      241 gctgcagagc gaaaatggca tgctgggcat cggccccttt cccaccgaag accaggtcga
      301 tgccgacctc atcaacgccg gcaagcagac cgtgaccacc ctgcccggct ccagcatctt
      361 cggcagccac gagagctttg cgatgatccg cggcgggcat atcgatctgc ccgtgctcgg
      421 cctcctgccg tacgtgacga ccgacgcgga cgtccgccgc cgcaagcgcc tccgcctgct
      481 ggcggcgacg gcggcggcgc tcctggcggc cgccagcagc ggcgtgttct gggtcctgag
      541 gctctggagg tacgtggcgt aatgggacgc atcgacgacg cgctgcggcg ctcgaacgtg
      601 gacgcggctc ggggaacggg agccgaagcg cccgcgcccg cgccgtcgcc ctggcaggtc
      661 gaggagcagg acatccgcgc ggcggcgccc ggggccgccg ggcccgccgg gacggccgcg
      721 cccgcagccg aaggcgccac gcgcggcgcg cggcgcgagc ccccgaaagc cgcccggttc
      781 gtcgtctccg gcggcatcga cgccagggcc gccgagcgcc tggtcgcatc cgccgacgcg
      841 agcccgctgc tcgtcgagca gttccggacg ctcggcgcca cgctgctccg catccagggc
      901 gagcggccgc tcaagagcct cctcgtcacc tcgccctcgc cgggcgacgg caagagccac
      961 gtggcgacga acctcgccct gaccctgagc gactcctacc atcggcgggt gctcctcatc
     1021 gacgccgacc tccggcggcc gacgctgcac caggtgttcc gcctggccaa cgccagcggc
     1081 ctctgcgagg ccctgcgcgc cgacaccgac gagaaagtgc ccacggcgcg cgtcagcgag
     1141 aacctgacgc tgctcccggc cggccggccc gaggccaacc ccctcgccgg cctctcctcg
     1201 ggccgcatgg cgcgcatcgt ggcggacgcc gcggccgcgt tcgactgggt gatcgtcgat
     1261 tcgccccccg tcgtcgtcct cgccgacgcg cacctcgtgt cggaaaccgt ggacggcgcg
     1321 atcgtcgtcg tgcgcgcggg cgtgacccgg ttccccgacc tcgcggccgc cgtggacacc
     1381 ctcggccagg atcgcgtgct gggcgtggtg ctcaacgcgc tggatccgtc cgagatgcgg
     1441 agcgagggct actacagcta ctactacggg cgctcggggc gtgcctcctc gaggcgttga
     1501 ctttcgtcgc cgcaatcaac gtcttttgat gtatcctcgg cgggaacagc ttcgtccacc
     1561 ggttcgcgtt cgcacttaac cccctgtcac aacacgcgtt agatgacgaa gcccggccgc
     1621 cttccccgtc gttcgtcgtt ggcgcatgcc ttgctgccgg atgaggctga gcccgccctg
     1681 cgtgcgaggc agcccgacat gccgaccgag gacatccgcc ggccgattcg tgagctcgag
     1741 tgcccgccag gcgaggagta cgacgaagac acgttcaatt acctcctcgg catggaacgg
     1801 gcgcgcgccg agcgcgcgag ccgccgggtc cgcctgctgc tggcgacgct cgaaggcgag
     1861 tccggcgaga gcgcgatgat cacgtcttcc tgcgcggccc gcctcttcgg cggcctgcgg
     1921 cagctcctgc gcgacacgga catcgtcggc tggtaccggc agaaccgcgt cgccggcgcc
     1981 gtgctgtgcg cgaggctcga cggctccgac gaagagacgt cccgcgccat cggcgcgcgc
     2041 gtcggcgaag gcctcaagcg gctccttccc gcccggacgg cgcgcaacct ccgcgtgcgg
     2101 gtgacccagc aggggccgcg gcgcgttccg aagggctgag agacccgagc cagtgacgaa
     2161 gtcgatcctg gaggcgcttc gggagttcgc ggactgggag aagtgcccgg gcccggtccc
     2221 ggcccccctg ccgctcgtgc agccgtcgcc cgggccccag gccggcatcc ccgtcctcgg
     2281 ggaaagcgtc ttcaaggacg tcctcgtccg cgagcgcaag tgcgccgacc gcttcgagtc
     2341 gccgttcgcc gtcgtactgg tggaccggag cgacgatgcc tcgggggcgg acgcgtgggt
     2401 cccgattctc ggggccatcg ccgcggtgaa gcgcgatgcc gacgccgtcg ggtggctcga
     2461 cgagggcaag gtgctgggcc tgctcctgcc cgacgtgccc ggcgagggcg cgctgaagat
     2521 cctcgatcgg ctgcgccacg aggtgtcggc ccgcctggga gatgccgcgc tggccgtctt
     2581 gtcgatgcgc gtctacgcgc accgggccgc gccggcggcc gacgggttct cgttcccgcc
     2641 cccggacctg ctgatcgaag cgttcgcgcc cgagccggcg cgcccgggcc gcgacgccgt
     2701 caagcgcgtc ctcgacatcg ccggcggcct cctgctgctc gtcgtcttct cgccgatcat
     2761 ggccgcagcc gccctggcgg tgaagctgac ctcggccggc cccgtgttcc acaggcaggt
     2821 gcgcgtcggc cggcgcggcg agccgttcac gatgctcaag ttccgcacca tgcacgccaa
     2881 cgccgggcac gccatccacc aggactacgt gacctggttc atcaagtcgg gcgggaaggg
     2941 ccggccgaac agcgacgggc tgttcaagct gacgaacgac ccccgcgtga cccgggcggg
     3001 ccggttcctg cgcaaggcga gcctcgacga gctgccccag ttcctcaacg tgctgcgcgg
     3061 cgagatgtcg ctcgtcgggc cgcggccgcc gctgcccttc gaggtggagc agtaccagcc
     3121 gtggcaccgc cggcgcgtcc tcgaggccaa gcccggcatc acagggccgt ggcaggtcca
     3181 gggccggagc cggacgagct tcgacgacat ggtgcgcctc gacctgcgct acgcccggac
     3241 gcacacgctc tggaccgacc tgaagatcct gcttgccacg ccccgcgcca tgttcaaggg
     3301 tgccgcctga tggatccgta cctctcgatt gcccccgacg tgaagctcgg gaagggcgtc
     3361 aagctctcca agttcatcaa cctgtacggc tgcgagatcg gcgacgagac gaagatcggg
     3421 gcctgcgtcg agatccagaa gggcgtgaag gtggggcggc gctgcaagat ctcgagccac
     3481 accttcatct gcgagggcgt gaccatcgag gacaacgtct tcgtcggcca cggcgtcacc
     3541 ttcaccaacg acgtctaccc gcgcgcgacg acggcggacg gcgagctgca gaccgaggcc
     3601 gactggaagg tggagcccac gctggtgaag aagggcgctt ccatcgggtc gggcgcgacc
     3661 atcctgcccc gcgtcaccat cggcgagcac gccatcgtcg gcgccggcag cgtcgtcacg
     3721 cgcaacgtgg cgccgcgcac catcgtgtgg ggcaaccccg cgcggttcct gcgccgcatt
     3781 cccccggaat cgggaggtca cacgtgagcc agcttcgcgt cggcgtcatc ggctacggct
     3841 actggggccc gaacatcgtc cgcaacctgt acggcatcga ggcgtgcagc gtggcggcgc
     3901 tttgcgacac caaccccgcc gcgctgcaga aggccggccg cgtctacccc gccctgcgca
     3961 cgacgccgga cccggccgag gtgctcgagt cgcccggcat cgacgccgtg gccatcgtca
     4021 cgcccgtgtg gacgcactac gagctggcgc gaaccgccct cgagaacggc aagcacgtgt
     4081 tcgtggagaa gcctttcacc tcgaccgtgc gccaggccga ggagctggtc gagctggccg
     4141 accgcaagaa cctccggatc atggtggacc acacgttcct gttcaccggc gccgtgcgga
     4201 agatccgcga gctcatcgac aagggcgtcc tcgggccgct gttctacttc gactcgtcgc
     4261 gcatcaacct gggcctcttc cagcacgacg tcagcgtggt gtgggacctg gcgccgcacg
     4321 atttgtccat catggactac gtgatcggca cgaaggccga ggccgtggtc gcgaccggcg
     4381 gcagccactt caaccaccag gccgacatgg ccttcctgac gatctacttc cccggcaacg
     4441 tcatcgcgca catcaacgtc aactggctct cgccggtgaa ggtgcgcacg acgctgatcg
     4501 gcggccgcga caagatgctc gtgtggaacg acctcgaggc cgacgagaag atcaaggtct
     4561 acgacaaggg cgtcgacgtg accagccgcg agggcgtcta cgatctgctc gtgagctacc
     4621 ggtccggcga cgtctgggcg cccaaggtgg accagaccga ggcgctcaag gccgagctgg
     4681 cctacttcgc cgagtgcgtc acgaccggga agaagccgtt caacgacggt gaggcgggcc
     4741 tgagcgtggt ccggatgctc gaggccgccg accagtcgct ggccgagcgc ggcaaggtcg
     4801 tgctgttgcc ctgatggagg ccgggccctc ctcgccccga gcgagcgcgc tcacgacggg
     4861 cgggccgaag tgaccccggc tcgcgtccct gcccatccac cccctcgtcg agtactaccg
     4921 ctgccccgag cacctcgcgg cgctcgggac ggcggatcct ctgccgctcg agtccggcta
     4981 cttccgcttc ggcggcgccg tctgctacgg ccggcaggcc gtcggggcgc cgtcgccgtc
     5041 gccggacggc ggcctcgtcg aagtgtcggg agcggtgtcc tcgtccggcg gccgcgtgct
     5101 gctgccgttc cagctcggcg aggccgtcgg caacctgcgc cacgagcggt accccgaggc
     5161 cctgcgggcc gtcgagcaca tctcgtcgtt cacgctgtcg catttcctct actacctggc
     5221 ccggcccttc ctgccggtgg gcgtcaggag gcacctgcag cggctgcact gggcgggctg
     5281 gcggcggatt ccgtttcccc gctggccggt ggacgcgagc gtcgagacga tcatgaagca
     5341 ggccgtccgc gccctgctcg aggccgggac ggtccgggag cttcctttca tctggttctg
     5401 gcccgacggc gcgccgggcg ccgccatgat gacgcacgac gtggaagggc ccgccggcgc
     5461 ggagttctgc ggcgagctga tggaccttga cgagcggtcc ggcatccgat cgtcgttcca
     5521 ggtgattccc gacggccccc gcgcggcgaa ggcggcgacc aggaagctgg tggacgacct
     5581 ccggcgccgc ggcttcgagg tgaacgtcca cgacctgacg cacgacgggc ggctgttccg
     5641 gaaccgcgat cgcttcctgc gccacgccgg ccggatcaac gcgaggggcc gcgagttcgg
     5701 cagccgcggc ttcaggtccg gcgccatgta ccggcgccag gagtggttct cggccctcga
     5761 catctcctac gacatgtccg tgccgaacgt cgcgcacctc gagccgcagg cgggcggctg
     5821 ctgcacggtg atgccgttct tcatgggcca cgtgctcgag ctgcccctga cggcggccca
     5881 ggactacacc gtgttccaca tcctgggcga gtactcgacg cggctgtggc gcgagcagat
     5941 cgacgcgatt ctggccgagc acggcttggc cagcttcatc gcgcacccgg actacctcgt
     6001 cgatccgcgc gcccgggagg tctacgccga gctgctgggc atcctgagcg gcctgcgcgc
     6061 cgagcgcggc gtctgggtcg cgctgccgtc ggagatcgac gggtggtggc gcgagcggcg
     6121 cgagatgcgc ctggcgcccg acggcggctc gtggcgcgtc gaagggcgcg gcgcggagcg
     6181 cgcccgcgtc gccttcgccc gtttccgcga cggttgcgtc gtgtacgagt tggagccgcg
     6241 agtcgcgaag tagatgcgtg atgggggccg ccgtccacgt cgtcgatccg ctcgccgacc
     6301 cgcgctggga cggcctcctc gcccgtcatc cccgcgcgtc cgtctttcac acgcgcggat
     6361 ggctcgacac gctgaaacgc acctacggct acgagccgtt cgccctgacg acgacggcgg
     6421 atggcccgct ggagaacggc ctggcgttgt gccgcgtgaa gacgccgttc gcgcgccggc
     6481 tggtgtcgct gcccttctcg gatcattgcg acccgctggt cgatcggccc gaagacgtct
     6541 cggcgatgct cgggttcctg aaggcgcagc aggagcgcga gcgctggcgg tcggtggagc
     6601 tgcggccccg ccaggccgcg tggggcctgg ctgccgaggc gcccgccgcc ggcggggatg
     6661 gggcgcggcc ctcgtggccg gccgagagcg cccgctacgt ccatcacgcg ctcgatctcg
     6721 cgcgccccgc ggagcaggtg ttcgccggct tccacccctc gcacacccag cgcgccgtgc
     6781 gacgcgccga gcgcgagggc ttgacgtacg aggccggcaa ctcggagccg ctcctgtccg
     6841 cgttctacgg gctgctgcgc ctgacccgcc gccgccacgg cctgccgccg cagccgcgtg
     6901 cgtggttccg gaacctcgcg gcgagcctgg gcgaccagtt ggccgtgcat ctggcgagca
     6961 aggacggccg gcccatcgcc gccatcctga cgctcggctt caagaggacc gtcgtctaca
     7021 agtacggcgc gtccgacgcc gcgttccatc ggctcggcgc catgccgttc ttgttcagtt
     7081 cgatgaccat ccgtgccgtc agccgcgcg
//
LOCUS       DAOSIF010000103         7102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9619_length_7102_cov_3.522492, whole genome shotgun sequence.
ACCESSION   DAOSIF010000103 DAOSIF010000000
VERSION     DAOSIF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9619_length_7102_cov_3.522492"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLT35_05015"
     CDS             <1..861
                     /locus_tag="PLT35_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28620.1"
                     /translation="RHDKGLLVNVVAVPLEPDAAGAEQADGWVVVFEDVTTRARLEEQ
                     LQISDKMASIGLLAAGVAHEVNTPLTGISSYTQMLLDRADPDDPQTKLLEKIERQTFR
                     AAKIVNGLLTLARGGRSGDERVPVDLNAVVSDVLSLLEHQLKAVNIQIRRDLDAAAPV
                     VHGVEHKLQQVFLNLFINARDAMPRGGWLSVATRAEGERVRVEVADTGAGIPQEHLAR
                     IYDPFFTTKGLGQGTGLGLSITYGIVGEHDGTIACDSRPGEGTRFTLTFPPAPSAQQR
                     AAAGGIPVGG"
     gene            869..2284
                     /locus_tag="PLT35_05020"
     CDS             869..2284
                     /locus_tag="PLT35_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG28621.1"
                     /translation="MARAGSILVIDDEEVMRDILEALLAPEGYDVRVASGGAEGLDLA
                     RSVPFDAALVDVMMPGMDGLTVLDELKKLDEDMAVVMVTAYASVDAAVDAMKRGALDY
                     ITKPFKNDEVLLVLRNAVERKRLVTENRSLRQNIQERYHRFDNIVGKSPSMRRVFDLI
                     IQAAPSRSTILIEGESGTGKELVARAIHTHSPRADRAFVTVNSGSLPPDLLESNLFGH
                     VKGAFTGAVYPKKGLFEVADRGSIFFDEIGSVPLETQPKLLRVIQEREFMRLGGVETI
                     KVDVRIIAATNIDLGRMMEEGQFREDLYYRLHVITIQLPPLRERRDDIPLLVRHFLQK
                     YGEENGKPELELAPEALDLMMAYQWPGNVRELENVIERGAVLTQGTRIGVELIPEHVR
                     HPPSFQVPAVAVPPDGLSLKDAVMGFEKRLIETTLESAGGVQKRAAELLRIKPTTLNE
                     MIKRHDIVTKRRRNGEEGGGE"
     gene            complement(2324..2728)
                     /locus_tag="PLT35_05025"
     CDS             complement(2324..2728)
                     /locus_tag="PLT35_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOG28622.1"
                     /translation="MKEKVGAEAKAGGDRAPARSFRDLIVWQKAHELVLATYRVSASF
                     PKTESFGLTAQIRSAAVSIAANIAEGFRRSGRSDKARMLNVAQGSLEEVRYYLILARD
                     LQYGGNAEIDGLLEDVSRLLNAYRRKLLTPLP"
     gene            complement(2808..4067)
                     /locus_tag="PLT35_05030"
     CDS             complement(2808..4067)
                     /locus_tag="PLT35_05030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28623.1"
                     /translation="MKIAFVIQRYGSEILGGSEYHCRLIAERLAERHDVEVLTTCARD
                     YITWKNEYPEGTDRVRGVTVRRFAVAHPRDIEAFNRYSDWIFHNPHSREDELEWLRQQ
                     GPWCPALVEHLERSHQAYDALIFFTYLYAPTVLGLAVAPGKSILVPTAHDEPAIHLGL
                     YQEVFALPAAIAYNTEVERRFLSRGFPIRAIAEETVGCGVDLPHQPYRRDRPAGAGAG
                     PAEGEEPAAGHAGRFAAPARGEAFRARHKIHGPFALYGGRIEKGKGCEELVEYFGTYH
                     ADGGHLTLALMGVKLMPLPEEPYIRFAGLLSERERLQALEAASVVVLPSPFESLSLMA
                     IEAFAVGTPVLANARCEVLVDHCLRSNAGLFYADRDEFVECLKLLAVDDRLRAQMGRN
                     GRAYVRRHYRWDLILGKYEKLMGAVRK"
     gene            complement(4064..5146)
                     /locus_tag="PLT35_05035"
     CDS             complement(4064..5146)
                     /locus_tag="PLT35_05035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28624.1"
                     /translation="MTVQQWLPAARRGDAVGDSARLMQALLRAQGHASEIFALEIEPD
                     LRRDVRPFRGHADAGGDVTLLHFATPSPLTGALGRLRHGRVVQYHNVTPPRFFAPFSP
                     ELAALSACARRELRARRELRALRDAADLAAGPSEFNRRELEALGFAETRVLPLAVDVG
                     RLRDAPPHPVMDRWLRDGRVNLLFVGRVAPNKKVEDLLRLAARYRRRVDPDARLVVAG
                     RDDSVPRYAEFLRLLAARLGLGADALVFTGALSDAALAACYRAAGAYVSMSEHEGFCA
                     PLVEAMAMDVPILAYAGAAVPETLGGAGVAFAPKDLDCAAEALGLLVHDARLRASVIE
                     GQRRRLAHFEIGRVGDALRRIVERFT"
     gene            complement(5202..6266)
                     /locus_tag="PLT35_05040"
     CDS             complement(5202..6266)
                     /locus_tag="PLT35_05040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28625.1"
                     /translation="MVINQWVPAAHRGDAIGDSARAVRALLRAQGHASEIFALTIDDD
                     MRGDARPFADPAASRGDVTILHFALPSPMTEAFARLPRGRVLQYHNITPARFYAAYDP
                     GIFRLAVLGRRELASLAGRTDLALGDSEFNRRELEALGFSPTGVFPIAVDARRITGAP
                     RTPSLEKWLDDGLTNFLFVGRMVPNKKVEDVIRLAEHYKRYVDAHYRFIFVGRHDGVP
                     RYTNMLRALIAELGWMPDRFLFTGPVPDCELAAYYRCSSVYISMSEHEGFCVPLLEAM
                     AADLPILAYSCTAIPDTLGGAGVQFAPKDFEHAAELLGMLAFDDGLRAAVIEGQRRRL
                     SDFSAGRMEREVRALVARCS"
     gene            complement(6277..>7102)
                     /locus_tag="PLT35_05045"
     CDS             complement(6277..>7102)
                     /locus_tag="PLT35_05045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28626.1"
                     /translation="GDSEFNRRELEALGFPETGVLPVVPDFAHLDGPPNDVVAREFDD
                     ERTNVLFVGRIIPNKRIENVIRFFAAYKARYNPRARLLLVGSYGGFERYLAMLQDLAA
                     RLHVGDVHFTGHVSDGELTAYYDVADVFLCASEHEGFCVPLVEAFYKRIPVLALARTA
                     VPATLDGAGVLYDEPDPADVAALLDAVAGDAGLRERILEGQDAALDRLRARDFAGTLT
                     GFVERVRRAPRRPAHRVAWDFWQQVDTAERLEQQRLFRPSLYKALPASARASGLGW"
BASE COUNT         1092 a         2502 c         2539 g          969 t
ORIGIN      
        1 cgccacgaca aggggctcct ggtcaacgtc gtcgccgtgc cgctcgagcc ggacgcggcc
       61 ggcgcggagc aggcggacgg ctgggtggtg gtgttcgagg acgtgacgac ccgcgcccgg
      121 ctcgaggagc agctgcagat ctccgacaag atggcgtcga tcggcctgct ggcggcgggc
      181 gtggcccacg aggtgaacac gccgctcacg gggatttcca gctacaccca gatgctcctc
      241 gaccgcgccg atccggacga tccgcagacg aagctcctcg agaagatcga gcgccagacg
      301 ttccgggcgg cgaagatcgt caacggcctg ctgacgttag cccgcggcgg ccgcagcggc
      361 gacgagcgcg tgcccgtcga tctgaacgcg gtcgtcagcg acgtcctctc gctgctcgag
      421 caccagctga aggcggtgaa catccagatc cgccgcgatc tcgatgcggc cgcgccggtg
      481 gtccacggcg tcgagcacaa gctgcagcag gtgttcctga acctgttcat caacgcgcgg
      541 gacgccatgc cgcgcggcgg ctggctgtcg gtggcgacca gggccgaggg cgagcgcgtg
      601 cgggtcgagg tggccgacac cggcgcgggc atcccgcagg agcacctcgc gcgcatctac
      661 gacccgttct tcaccaccaa gggcctcggc caggggaccg gcctcggcct ctccatcacg
      721 tacggcatcg tcggggagca cgacggcacc atcgcgtgcg acagccgccc cggcgagggg
      781 acgcgcttca cgctgacgtt cccgcccgcg ccctcggcgc agcagcgcgc ggcggcgggc
      841 ggcatcccgg tgggcgggta gcaggcccat ggcgcgcgcg ggatccatcc tggtcatcga
      901 cgacgaggag gtcatgcggg acatcctcga ggcgctcctg gcgcccgagg gctacgacgt
      961 ccgcgtcgcc tcgggcgggg ccgagggcct cgacctggcg cgctccgtcc cgttcgacgc
     1021 ggcgctggtg gacgtgatga tgccgggcat ggacgggctc accgtcctcg acgagctgaa
     1081 gaagctggac gaagacatgg ccgtcgtgat ggtcaccgcc tacgcctcgg tggacgccgc
     1141 cgtggacgcg atgaagcgag gggcgctgga ctacatcacg aagccgttca agaacgacga
     1201 ggtcctgctt gtcctgcgca acgccgtcga gcggaagcgg ctcgtgaccg agaaccggtc
     1261 gctccgccag aacatccagg agcgctacca ccggttcgac aacatcgtcg gcaagagccc
     1321 gtcgatgcgt cgcgtgttcg acctgatcat ccaggcggcg cccagccggt ccacgatcct
     1381 catcgagggc gagagcggca ccggcaagga gctcgtcgcc cgcgcgatcc acacgcactc
     1441 gccgcgggcg gaccgcgcgt tcgtcaccgt gaactccggc agcctgccgc cggacctgct
     1501 cgagtcgaac ctgttcggcc acgtcaaggg cgccttcacc ggggcggtgt acccgaagaa
     1561 gggcctcttc gaggtcgccg accgcggcag catcttcttc gacgagatcg gcagcgttcc
     1621 cctcgaaacg cagccgaagc tcctgcgggt catccaggag cgcgagttca tgcggctggg
     1681 cggcgtcgag accatcaagg tggacgtgcg catcatcgcc gccacgaaca tcgatctcgg
     1741 ccggatgatg gaggaggggc agttccgcga ggacctgtac taccggctgc acgtcatcac
     1801 catccagctg ccgccgctgc gcgagcgccg ggacgacatc ccgctgctcg tccgccactt
     1861 cctgcagaag tacggcgagg agaacgggaa gccggagctc gagctcgcgc cggaggccct
     1921 cgatctcatg atggcgtacc agtggcccgg caacgtgcgc gagctcgaga acgtgatcga
     1981 gcgcggggcg gtgctcacgc aggggacccg gatcggcgtc gagctgatcc ccgagcacgt
     2041 gcgccatccg ccctcgtttc aggtcccggc cgtcgccgtc ccgcccgacg ggctctcgct
     2101 gaaggacgcc gtgatgggct tcgagaagcg cctcatcgag acgacgctcg agagcgccgg
     2161 aggcgtccag aagcgcgccg ccgagctgct ccgcatcaag cccacgacgc tcaacgagat
     2221 gatcaagcgc cacgacatcg tgacgaagcg aagaaggaat ggggaggagg ggggagggga
     2281 gtaggaggta ggggaggtac aggaggtaga gaaggtacag gagtcagggg aggggagtca
     2341 ggagtttgcg gcgataggca ttgagcaggc ggctgacatc ctccaagaga ccgtcgatct
     2401 cagcgttccc accgtattgc aggtcccggg cgagaatcag atagtagcgg acctcctcca
     2461 gggacccttg ggcgacattc agcattcgtg ctttgtcaga ccggcctgac cgccggaatc
     2521 cttccgcaat gttcgcggca atcgacaccg cagcagaccg aatctgagcc gtgaggccga
     2581 agctctctgt cttcggaaag ctcgcgctga ctcggtaggt ggccagcacg agttcgtgcg
     2641 ccttctgcca gacgatgaga tcgcggaagc tccgggctgg cgcccggtcc cctccagcct
     2701 ttgcttcagc ccccaccttc tccttcaccc gcctcccctc ccttctttct cttctttccc
     2761 tttcttccct tctctacctt ctctaccttc cctaccttct ttaccttcta cttcctcacc
     2821 gcccccatca gcttctcgta cttccccagg atgagatccc agcggtagtg ccggcggacg
     2881 taggcgcgtc cgttgcggcc catctgcgcg cgcaggcggt cgtccacggc caggagcttc
     2941 aggcactcga cgaactcgtc gcggtcggcg tagaacagcc cggcgttgct ccgcaggcag
     3001 tggtcgacca ggacttcgca gcgggcgttg gccaggaccg gcgtgccgac ggcgaaggcc
     3061 tcgatggcca tgagcgacag gctctcgaac ggcgagggca gcacgacgac ggaggcggcc
     3121 tcgagggcct ggaggcgctc gcgctccgag agcaggcccg cgaagcggat gtacggctcc
     3181 tccggcagcg gcatcagctt gacgcccatg agggcgagcg tcaggtggcc gccgtcggcg
     3241 tggtacgtgc cgaagtactc gaccagctcc tcgcagccct tgcccttctc gatgcggccg
     3301 ccgtagagcg cgaagggccc gtggatcttg tggcgcgccc tgaacgcctc gccgcgggcc
     3361 ggggccgcga agcggcccgc gtggcccgcg gcgggctcct cgccctccgc cgggccggcc
     3421 ccggcgccgg ccgggcgatc gcgccggtac ggctggtgcg gcaggtccac cccgcagccc
     3481 acggtttcct ccgcgatcgc gcggatgggg aagccccggc tgaggaagcg gcgctcgacc
     3541 tcggtgttgt aggcgatggc cgccggcagg gcgaacacct cctggtagag gccgaggtgg
     3601 atggccggct cgtcgtgggc ggtcggcacc aggatgctct tgccgggcgc gacggccagg
     3661 cccagcacgg tcggggcgta gaggtacgtg aagaagatca gcgcgtcgta cgcctggtgc
     3721 gagcgctcga ggtgctcgac gagggccggg caccacggcc cctgctgccg aagccactcg
     3781 agctcgtcct cgcgcgagtg cgggttgtgg aagatccagt ccgagtaccg gttgaacgct
     3841 tcgatgtccc gcgggtgcgc cacggcgaag cggcgcacgg tgacgccgcg cacccggtcc
     3901 gtcccttcgg ggtactcgtt cttccacgtg atgtagtcgc gggcgcacgt ggtcagcacc
     3961 tcgacgtcgt gccgctcggc cagccgctcg gcgatcaggc ggcagtggta ctcggatccc
     4021 cccaggatct ccgagccgta gcgctggatg acgaacgcga tcttcatgtg aaccgttcca
     4081 cgatccgccg cagggcgtcg ccgacgcggc cgatctcgaa gtgggcgagg cgccgccgct
     4141 ggccctcgat gaccgaggcg cggaggcggg cgtcgtggac gagcaggccg agcgcctcgg
     4201 ctgcgcagtc caggtccttg ggggcgaacg ccacgccggc gccgcccagg gtctccggca
     4261 cggccgcccc cgcgtaggcc aggatcggca cgtccatcgc catggcctcg accagcggcg
     4321 cgcagaagcc ctcgtgctcg ctcatcgaga cgtacgcgcc ggcggcgcgg tagcaggccg
     4381 ccagcgccgc atccgacagg gcgcccgtga agacgagggc gtcggcgccg aggccgaggc
     4441 gggcggccag cagcctcagg aattcggcgt agcgcgggac gctgtcgtcg cggccggcca
     4501 ccacgaggcg cgcgtccgga tcgacgcgcc tccggtaccg cgccgccagg cgcagcaggt
     4561 cctcgacctt cttgttgggc gcgacgcggc cgacaaacag caggttgaca cggccgtcgc
     4621 gcagccagcg atccatcacc gggtgcggcg gggcgtccct cagccgtccc acgtccacgg
     4681 cgaggggcag caccctcgtc tcggcgaagc caagggcctc cagctcgcgg cggttgaact
     4741 cggacggccc ggcggccagg tcggccgcgt cgcgcagggc gcggagctcc cgccgggcgc
     4801 ggagctcccg ccgggcgcag gcggagaggg cggccagctc gggcgagaac ggcgcgaaga
     4861 agcgcggcgg cgtgacgttg tggtactgca ccacgcggcc gtgccgcagc cggccgaggg
     4921 cgccggtcag gggcgacggc gtcgcgaaat gaaggagcgt cacgtcgccc cccgcatcgg
     4981 cgtggccgcg aaacggccgc acgtcccgcc gcagatcggg ctcgatctcc agcgcgaaga
     5041 tctcggaagc gtggccctgc gcgcgcagca gcgcctgcat gaggcgggcg ctgtcgccga
     5101 cggcgtcgcc gcggcgggcc gcgggcagcc actgctggac cgtcatcggc gccccgcccc
     5161 gcccggccgc gcggcccggg cgccgcccgc tcctcggaac gtcacgagca cctcgcgacg
     5221 agcgcccgca cctcgcgctc catgcgcccg gccgagaagt ccgagagccg gcgccgctgg
     5281 ccctcgatca cggcggcccg caggccgtcg tcgaaggcga gcatgccgag cagctcggcg
     5341 gcgtgttcga agtccttcgg cgcgaactgc acgccggcgc cgcccagcgt gtccgggatc
     5401 gcggtgcacg agtaggcgag gatcggcaga tcggccgcca tcgcctccag cagcggcacg
     5461 cagaacccct cgtgctcgct catcgagatg tacacgctcg agcagcggta gtaggccgcc
     5521 agctcgcagt cggggaccgg gccggtgaac aggaaccggt ccggcatcca gccgagctcg
     5581 gcgatgagcg cgcgcagcat gttcgtgtag cgcggcaccc cgtcgtgccg gccgacgaag
     5641 atgaaccggt agtgcgcgtc cacgtagcgc ttgtagtgct cggcgaggcg gatgacgtcc
     5701 tcgaccttct tgttgggcac catgcgcccg acgaacagga agttcgtcag cccgtcgtcg
     5761 agccacttct cgagggacgg cgtccgcggc gcgccggtga tgcggcgcgc gtcgacggcg
     5821 atcgggaaga cgcccgtggg cgaaaaaccg agcgcctcca gctcgcgcct gttgaactcc
     5881 gagtcgccca gcgccaggtc cgtgcggccg gcgagcgagg ccagctcgcg gcgtccgagc
     5941 accgccaggc ggaagatgcc gggatcgtac gcggcgtaga agcgcgccgg cgtgatgttg
     6001 tggtactgca ggacgcggcc gcgcggcagg cgggcgaacg cctcggtcat cggcgagggc
     6061 agggcgaagt gcaggatggt gacgtcgccc cgcgaggcgg ccgggtcggc gaagggccgc
     6121 gcgtcgcctc gcatgtcgtc gtcgatcgtg agggcgaaga tctccgacgc gtggccctgc
     6181 gcccggagca gcgcgcgcac cgcccgcgcg ctgtcgccga tggcgtcgcc ccgatgcgcc
     6241 gccggcaccc actggttgat caccatcgtg gcccgcctac cagccgaggc cgctggcccg
     6301 cgccgaggcc ggcagggcct tgtagagcga cggccggaag agccgctgct gctcgagccg
     6361 ctcggccgtg tccacctgct gccagaagtc ccaggccacg cggtgcgcgg gccggcgagg
     6421 cgcgcgccgg acgcgctcca cgaagccggt gagcgtgccc gcgaagtcgc gggcccgcag
     6481 gcgatcgagc gcggcgtcct ggccctccag gatccgctcc cgcagcccgg cgtcgccggc
     6541 gacggcgtcg aggagcgccg cgacatcggc cggatcgggc tcgtcgtaca gcaccccggc
     6601 accgtcgagg gtcgccggca ccgccgtgcg cgccagggcc aggacgggga tccgcttgta
     6661 gaacgcctcg acgagcggca cgcagaaccc ctcgtgctcg ctcgcgcaga gaaagacgtc
     6721 ggcgacgtcg tagtaggccg tcagctcccc gtccgacacg tggcccgtga agtgaacgtc
     6781 gccgacgtgc agccgcgccg ccaggtcctg cagcatcgcc agataccgct cgaagccccc
     6841 gtacgagccc acgaggagca gccgggcgcg cgggttgtag cgcgccttgt aggccgcgaa
     6901 gaaccggatg acgttctcga tccgcttgtt cgggatgatg cggcccacaa acagcacgtt
     6961 cgtccgctcg tcgtcgaact cccgcgccac gacgtcgttg ggcgggccgt ccaggtgcgc
     7021 gaagtccggc acgacgggca gcacgcccgt ctcgggaaag ccgagcgcct ccagctcgcg
     7081 cctgttgaac tccgagtcgc cc
//
LOCUS       DAOSIF010000104         7098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9623_length_7098_cov_3.208718, whole genome shotgun sequence.
ACCESSION   DAOSIF010000104 DAOSIF010000000
VERSION     DAOSIF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9623_length_7098_cov_3.208718"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PLT35_05050"
     CDS             <1..1171
                     /locus_tag="PLT35_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG28627.1"
                     /translation="NGGGAAFCGDCPLKLPLGDGDAPPQRGLDVPPQPSRLYVECTAA
                     CNISCFKACCAPETGITRTRQAGMLDAGLFGRVLDEAGPFLGRIDFFNYGEAFLHKRA
                     VEMCERVKRDFPHIYLYTSTNGLALDEESARRLARSGIDEVTFSIDGASQATYERYRR
                     RGRFETAIANLRAMAAEKRAAGRDVPVLNWRYILFEWNDSDEEMQRARDLAAEIGVDR
                     LAWEITDHPEEAFSRRFAPGTAEHQRIRHEIWDTSNLGNAIPGATPRARIEVAPMVPG
                     LPLFARRGRLFAVRTRVHNLSTRPFPREASYGRRLVRLGAQLCDASGIVLDRDHARAW
                     LPRPIGPGEQVAVPIEVLAPPQSGRYQLKFDLVSEGIDWFEACGSDTTLRPLVVL"
     gene            1425..1757
                     /locus_tag="PLT35_05055"
     CDS             1425..1757
                     /locus_tag="PLT35_05055"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG28628.1"
                     /translation="MSSSGWRKAWWRQTADAVIVTDRNGVIEYVNPAVERMTGFAPKD
                     LTDVRAALKRASKLSLARRVQQRMHPGPPPQGSGWDVAGAAIPADETSGGSGTRHGSR
                     CRCGSRTT"
     gene            complement(1905..2804)
                     /gene="lpxC"
                     /locus_tag="PLT35_05060"
     CDS             complement(1905..2804)
                     /gene="lpxC"
                     /locus_tag="PLT35_05060"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOG28629.1"
                     /translation="MAFQRTLRRQVTCAGIGLHSGQKVTLTLKPAPADSGIRFRRTDL
                     GVEIPASVAHVSGTNHATGLTRDGAVVETVEHLLAAMTCLGLDNAVVEIDHREVPVMD
                     GSSAPFVYLIQEAGLRTLPAPRRYLKVLRPITVSRGDKLMSLYPSDYFKVTYSISFDH
                     PLLRHQSHTLRVNEGAFVDGIAPARTFGFLNEVELLRQQGLALGGSLENAIVLSETGV
                     LNNQLRYDDEFVRHKILDAIGDLSLVGHPIIGHLVAHRAGHALHTAFAKRILEERDAW
                     KLVELVPEQAPAFAPAAVPVKAG"
     gene            complement(3002..5947)
                     /gene="uvrA"
                     /locus_tag="PLT35_05065"
     CDS             complement(3002..5947)
                     /gene="uvrA"
                     /locus_tag="PLT35_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG28630.1"
                     /translation="MTHRQITIRGARVHNLKNIDVDLPRNQLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLEQMEKPDVDSIEGLSPALSIEQKTTGANPRSTVGT
                     VTEIYDYLRLLFANTGVPHCPQCGLPITSQSVEQIVAQVLASPPDTRINVLAPVVRGR
                     KGEFKKDLAAWRQRGFLKARVDGQLRALEEDIALDRRRNHSIEIVVDRVVIRPGIERR
                     LAESVEIALGLADDIVLVNSLAAGDRLFSRKLACVSCGLSIPELTPRAFSFNSPHGGC
                     PECQGLGALWDFDPERIVPDPDRSLAEGAVAPWAAGDRRLVREAVAALGRNFGIDITV
                     PFGRLPKRVRDLLLYGPAAEAKTNGRGAGRGGPGGGAPAAAEDGPAAGRRGSAASARA
                     AAARRLKDPYGKGFEGIIPNLRRRYDQATWVDQADIERFRSLRPCPACQGERLRPESR
                     AVRVKGRTLPQVVSLPISAALEAFETDQLTAREELIAGRIHREIVERLRFLVDVGVGY
                     LTLGRSAATLSGGEAQRIRLATQIGANLRGVLYVLDEPSIGLHQRDNRRLLATLRRLR
                     DLGNTVIVVEHDEETIRSADYVVDLGPGAGEHGGRVIFQGTPQALLREGRASLTGQYL
                     RGERMIGVPRARRAALKGELVIRGARANNLKRIDVRIPLGLLTAITGVSGSGKSTLVN
                     DILYRALARSLYRANDEPGAHDRIEGIELIDKVIEIDQSPIGRTPRSNPATYTGLFTF
                     IRELFALVPEARARGFKPGRFSFNVKGGRCETCQGDGVIAIEMHFLPDVYVTCEECKG
                     RRYNRETLEITYRGRSVADMLDLTVDQALPLVEHFPSVAGKLRTLQDVGLGYIKLGQS
                     ATTLSGGEAQRVKLAKELSKRGTGRTLYILDEPTTGLHFDDVKKLLDVLNKLVDQGNT
                     VVVIEHNLDVVKTADHLIDLGPDGGEAGGKVVAAGTPESVATEPASYTGQVLAAVLNR
                     AAGQCPERG"
     gene            6172..>7098
                     /locus_tag="PLT35_05070"
     CDS             6172..>7098
                     /locus_tag="PLT35_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HOG28631.1"
                     /translation="MGLVVGRSVLRSDGAAKAAGTARYVADLARPGMLHARTIRSTIP
                     CGEVTRIRLDFDRAGFTIVDARDIPGRNVISLVADDQPCLADGVVRHVAEPVLLLAHE
                     DRERLIEAEVRIEYRPGTAVLDPAASGRAFKTVSIAKGDLAAGFRRADFVVEGEYRTG
                     HQEHLYIEPNGMLAVPENGGVTIIGSMQCPYYVHRALTALLGLAPDKVRVAQAETGGA
                     FGGKEDYPSMIAGHAALVALKSGRPVKLVYDRAEDLLATTKRHPSVVRHRTGVTRDGR
                     ITAMDIEVLLDGGATPIGLESTARWHGRDSGLD"
BASE COUNT         1071 a         2579 c         2401 g         1047 t
ORIGIN      
        1 gaacggcggc ggcgccgcct tctgcggtga ctgtccgctg aagctccccc tcggcgacgg
       61 cgacgccccg ccgcagcgcg gcctcgacgt cccaccgcag ccgtcgcgcc tctacgtcga
      121 gtgcaccgcc gcctgcaaca tctcgtgctt caaggcctgc tgcgcgcccg aaaccggcat
      181 cacccgcacg cggcaggcgg gcatgctcga tgccggcctg ttcgggcgcg tgctcgacga
      241 agccggcccc ttcctcggcc gcatcgactt cttcaactac ggcgaggcct tcctccacaa
      301 gcgcgccgtc gagatgtgcg aacgcgtgaa gcgcgacttc ccgcacatct atctctacac
      361 gagcaccaac ggcctggcgc tcgacgagga gtccgcgcgg cggctcgcgc ggtcgggcat
      421 cgacgaggtc accttctcca tcgacggcgc ctcgcaggcc acctacgagc gctaccgccg
      481 gcgcgggcgc ttcgagaccg ccatcgccaa cctgcgcgcg atggccgccg agaagcgcgc
      541 cgcgggccgg gacgtgcccg tgctgaactg gcgctacatc ctcttcgagt ggaacgacag
      601 cgacgaggag atgcagcgcg cgcgggacct ggcggccgag attggcgtcg atcgcctggc
      661 ctgggagatc accgatcatc cggaggaggc gttctcgcgc cggttcgcgc ccggaacagc
      721 cgagcaccag cgcatccgcc acgagatctg ggacacgagc aacctcggca acgccatccc
      781 cggggcgacc ccgcgggccc gcatcgaggt ggcgccgatg gtccccggcc tgccgctctt
      841 cgccaggcgc ggccggctct tcgccgtgcg cacgcgcgtc cacaacctct ccacccggcc
      901 cttcccgcgc gaagccagct acggccgccg gctcgtccgc ctcggcgccc agctctgcga
      961 cgcctccggg atcgtgctcg accgcgacca cgcccgggcg tggctgccac gcccaatcgg
     1021 ccccggcgag caggtggccg tgcccatcga agtgctggcc ccgccgcagt ccggacgcta
     1081 ccagctcaag ttcgacctgg tcagcgaagg catcgactgg ttcgaagcct gcggatccga
     1141 cacgaccctc aggcccctcg tcgtgctctg accgcccctc gcgagacgag ccgtcgaagg
     1201 ggagccgcca gccgccagtg aatctgaaaa cactggtcgg taattgagcc cactaggaag
     1261 gacgagtcac ccggggcgcc tggccccgcc gctcgcgtcg tgccgccggc agcagctggg
     1321 atcgtgccgc cctcgtccag gcgagccaga agcgaacgcc atgtccgacg agtcccgggt
     1381 cgagcggccg ctctcgccgt ttcccgtcga cggcttcttg aaggatctcc tcgagtgggt
     1441 ggcggaaggc gtggtggcgg cagacggccg acgccgtcat cgtgaccgac cgaaacggcg
     1501 tgatcgagta cgtgaatcca gcggtcgagc gcatgaccgg cttcgcgccc aaggacctga
     1561 ccgacgtccg cgccgcgctg aagcgggcaa gcaagctgag cctggcccgc cgggtgcagc
     1621 agcggatgca ccccgggccg ccgccccagg gcagcggctg ggacgttgcc ggcgccgcca
     1681 ttccggccga tgaaacgtca ggaggatccg ggacgcggca cggcagcaga tgccgatgcg
     1741 gcagccggac gacatgacga tgctggtgtg cgggctgcgc gcggagagct gacggccggc
     1801 acacccccgc ctgcggccag ccccgcgcgg cggcagggcc gcacgcgccg cgccggcacc
     1861 tgcggccctg tctcctggag gaggaagatg agcggactga gcgactagcc ggccttcacc
     1921 ggcacggccg ccggcgcgaa cgccggggcc tgttcgggca ccagctccac gagcttccac
     1981 gcgtcacgct cctcgaggat ccgcttcgcg aaagccgtgt gcagcgcgtg gccggcccga
     2041 tgggccacca ggtgcccgat gatcgggtgc ccgacgagcg acagatcgcc gatggcatcc
     2101 aggatcttgt ggcgcacgaa ctcgtcgtcg tagcgcagct ggttgttcag cacgccggtc
     2161 tcgctcagca cgatcgcgtt ttcgagcgac ccgccaagcg ccaggccttg ctgacgcagc
     2221 agctcgacct cgttcaggaa cccgaacgtc cgggccggcg cgatgccgtc cacgaacgcg
     2281 ccctcgttga cgcgcagcgt gtgcgactgg tggcgcagga gcgggtggtc gaagctgatg
     2341 ctgtaggtga ccttgaagta gtccgacggg tagagcgaca tcagtttgtc gccccgcgac
     2401 acggtgatgg gccgcagcac cttcaagtag cgccgcggcg ccggcagcgt ccgcagaccg
     2461 gcttcctgga tgaggtacac aaacggcgcc gaactcccgt ccatgacggg aacctcgcgg
     2521 tggtcgatct cgaccacggc gttgtccaag cccaggcagg tcatcgcagc cagcaggtgc
     2581 tcgaccgtct cgacgaccgc cccgtcgcgc gtcaaccctg tggcgtggtt cgtcccgctc
     2641 acatgcgcca ccgacgcggg aatctccacg ccaaggtccg tgcggcggaa ccggatgccg
     2701 gagtccgcgg gagcaggctt gagcgtcagc gtgactttct gaccggaatg gagtccgatg
     2761 ccggcgcacg tgacctggcg ccgtagtgtc cgttgaaagg ccatgatcct ctcgtcggtt
     2821 caggagctag aaccgccctc tactacgcaa actccatacc ggcatggatc ctgtcgcacg
     2881 gagtcgagta agtcatattg atatcaacag ttaagtcaac acgctctcgt ttcggacgac
     2941 ttccactcga ccgctagtgc agtcaatcag acacaccaca ccgcccctca aacgtggtaa
     3001 ttcagccacg ttcagggcat tgaccggccg cccggttcaa cacggctgcg agcacctggc
     3061 ccgtataaga cgctggctcg gtcgccacgg actccggggt cccggccgcc acgaccttcc
     3121 cgcccgcttc cccgccgtcc ggaccgaggt cgatcaggtg gtccgccgtt ttgacgacgt
     3181 cgaggttgtg ctcgatcacg acgacggtgt tgccctggtc caccagcttg ttcagcacgt
     3241 ccagcagctt cttcacgtcg tcgaagtgca gccccgtcgt gggctcgtcg aggatgtaca
     3301 gcgtccgtcc cgttccgcgc ttcgacagtt ccttcgcgag cttgacgcgc tgcgcctcgc
     3361 cgccgctgag cgtcgtggcc gactgcccga gcttgatgta gcccaggccc acgtcctgca
     3421 gtgtcctgag cttgccggcg actgacggga agtgctcgac cagcggcagg gcctggtcga
     3481 ccgtcaggtc gagcatgtcg gccaccgatc ggccgcggta cgtgatctcg agcgtctcgc
     3541 ggttgtaccg gcggcccttg cattcctcgc acgtgacgta gacgtcgggc aggaagtgca
     3601 tctcgatggc gatcacgccg tctccctggc aggtctcgca gcggccgccc ttcacgttga
     3661 aggagaagcg cccgggcttg aagcccctgg cgcgggcctc cggcaccagg gcgaacagct
     3721 cgcggatgaa ggtgaacagc cccgtgtacg tcgcggggtt ggaccgcggc gtccggccga
     3781 tgggcgattg atcgatctcg atgaccttgt cgatgagctc gatgccctcg attcgatcgt
     3841 gggcgccggg ctcgtcgttc gcgcggtaga gcgagcgcgc gagcgcgcgg tacaggatgt
     3901 cgttcaccag cgtggacttg cccgacccgc tgacgccggt gatcgccgtg agcagcccga
     3961 gcgggatgcg cacgtcgatg cgcttgagat tgttggcccg cgcgccccgg atcaccagct
     4021 cgcctttgag cgccgcgcgc cgcgcgcgcg gcacgccgat catgcgttcg ccccgcaggt
     4081 actggccggt gagcgacgcg cggccctcgc gcagcagggc ctggggcgtc ccctggaaga
     4141 tgacacggcc gccgtgctcg ccggcccctg gccccaggtc cacgacgtag tcggccgagc
     4201 ggatggtctc ctcgtcgtgc tcgacgacga tgaccgtgtt gccgaggtcg cggagccggc
     4261 ggagcgtcgc gagcagccgc cggttgtcgc gctggtgcag cccgatcgac ggctcgtcca
     4321 gcacgtacag cacgccgcgc agattcgcgc cgatctgcgt cgccaggcgg atgcgctgcg
     4381 cctccccgcc cgagagcgtg gcggcgctgc ggccgagcgt caggtagccg acgccgacgt
     4441 ccacgaggaa gcgcagccgc tcgacgatct cgcggtggat gcgtcccgcg atcagctcct
     4501 cgcgcgccgt caactggtcc gtctcgaacg cctcgagcgc cgcggaaatc ggcagcgaca
     4561 ccacctgcgg cagcgtgcgc cctttcaccc gcacggcccg gctctcgggc cgaagccgct
     4621 cgccctggca cgcggggcac ggccggagcg aacggaaccg ctcgatgtcg gcctgatcca
     4681 cccacgtcgc ctggtcgtag cgccggcgga ggttcggaat gatgccctcg aaccccttgc
     4741 cgtatggatc cttcagccgc ctcgcggccg ctgcgcgggc cgacgccgcg gagccccgtc
     4801 ggcccgccgc cggcccgtct tccgcggcgg ccggtgcgcc gccgcccggc ccgccgcgcc
     4861 cggccccccg gccgttcgtc ttcgcctcgg cggccggtcc gtagagcagc aagtcgcgca
     4921 ctcgcttcgg cagccggccg aacggcaccg tgatgtcgat gccgaagttg cgaccgaggg
     4981 ccgccaccgc ctcgcgcacc aggcgccggt ccccggctgc ccacggcgcg acggcccctt
     5041 ccgccagcga gcggtccgga tccggcacga tccgctcagg atcgaagtcc cacagcgcgc
     5101 cgaggccctg gcattcggga catccgccat ggggcgaatt gaaggagaag gcgcgcggcg
     5161 tgagctcggg gatgctcagc ccgcacgaca cgcaggcgag cttccgcgag aacaggcgat
     5221 ccccggccgc gaggctgttg acgagcacga tgtcgtcggc cagcccgagg gcgatctcga
     5281 ccgactccgc cagccggcgc tcgatcccgg gccggatgac cacccggtcc accacgatct
     5341 cgatggagtg attgcggcgt cggtcgaggg caatgtcctc ctcaagggcg cgcaactggc
     5401 cgtccacccg tgccttcagg aatccccgct gccgccaggc ggccagatcc ttcttgaact
     5461 cgcccttccg gcctcggacg accggggcca gcacgttgat ccgcgtgtcc ggcgggcttg
     5521 ccagcacctg cgccacgatc tgttcgacgg actgcgacgt gatgggaagg ccgcactgcg
     5581 ggcagtgcgg cacgccggtg ttcgcgaaca acagccggag gtagtcgtag atctcggtga
     5641 cggtgcccac cgtcgagcgg gggttggcgc ccgtcgtctt ctgctcgatc gagagcgcgg
     5701 gcgacaggcc ctcgatcgaa tcgacgtcgg gcttctccat ctgctcgagg aactgccggg
     5761 cgtacgccga gagcgactcg acgtagcggc gctgcccctc ggcgtagatc gtgtcgaagg
     5821 cgagcgagga cttgccggat cccgacaggc ccgtgatgac cacgagctgg ttgcgcggca
     5881 ggtccacgtc gatgttcttc aggttgtgga cgcgcgcgcc gcggatggtg atctgccgat
     5941 gggtcacaat cgattcgact cgggagtcgg ggctcggggc ccggaggacc ggactcgggg
     6001 tcgagccccg gcccgcccct cctcacaagc ccaggccagg ccctgcccaa accgaagatc
     6061 ttaccacgcg cgggcgttat aatcgcggca cccggcggcg ggcgggcgga ccctcctggt
     6121 ccccgtcgtc ccgccccggt gacctcgcca tggaggctgc cggaggctga catgggcctc
     6181 gtcgtcgggc gcagtgtcct ccgcagcgac ggagccgcca aagccgcagg caccgcgcgc
     6241 tacgtcgcgg acctggcgcg cccgggcatg ctgcatgccc gcacgatccg ctccaccatc
     6301 ccctgcggcg aggtgacgcg gatccggctc gatttcgacc gcgccggatt cacgatcgtg
     6361 gacgcccgcg acatccccgg ccgcaacgtc atctccctcg tcgccgacga ccagccgtgc
     6421 ctcgccgacg gcgtcgtccg ccacgtcgcc gaacctgtcc tgctgctggc ccacgaagac
     6481 cgagagcggc tgatcgaggc cgaggtccgg atcgagtacc gacccggcac ggccgtcctc
     6541 gacccggcgg cgtcgggccg cgcgttcaag accgtgtcca tcgcaaaagg cgaccttgcc
     6601 gccgggttcc gccgggccga cttcgtcgtc gagggcgagt accgcaccgg ccaccaagag
     6661 cacctctaca ttgagcccaa cggcatgctg gccgtgccgg agaatggcgg cgtgacgatc
     6721 atcggctcga tgcagtgccc gtactacgtg caccgcgccc tgaccgcgct gctcgggctg
     6781 gcgcccgaca aggtgcgcgt cgcgcaggcc gagaccgggg gcgcgttcgg cggcaaggag
     6841 gactacccgt cgatgatcgc cggccatgcg gcgctcgtcg cgctgaagtc gggccggccg
     6901 gtgaagctcg tgtacgaccg cgccgaggac ctgctcgcga cgacgaagcg gcacccgtcc
     6961 gtggtgcgcc accggaccgg cgtgacgcgc gacgggcgga tcacagccat ggacatcgag
     7021 gtgctgctcg acggcggcgc gaccccgatc gggctcgagt cgaccgctcg atggcacggt
     7081 cgagattctg ggctcgac
//
LOCUS       DAOSIF010000105         7020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9755_length_7020_cov_3.981335, whole genome shotgun sequence.
ACCESSION   DAOSIF010000105 DAOSIF010000000
VERSION     DAOSIF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9755_length_7020_cov_3.981335"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLT35_05075"
     CDS             complement(<1..296)
                     /locus_tag="PLT35_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28632.1"
                     /translation="MPGERLTPAQILDLLPQQRPFRFVDEILEVDEQRITGRYTFRPD
                     EFFYAGHFPGRPITPGVILLESMGQVGVVALGIYLFALDYPDEDPSRWTTLFVEA"
     gene            complement(333..923)
                     /gene="fabA"
                     /locus_tag="PLT35_05080"
     CDS             complement(333..923)
                     /gene="fabA"
                     /locus_tag="PLT35_05080"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF003509.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HOG28633.1"
                     /translation="MTYAEFQQRASFSKEEVLACAFGTLVTDPPSEGFASLPGPPMLM
                     VDRVVEISHRGPQGRIVAEYDVAVDAWFFQCHFRGDPVQPGCLGVDAVWQLIGLYGAV
                     RGAKGGGRALGAKEIEFFGQIRPYNTLVRYEIDIRRYAELAGSGSAIAIGTGTVLVDG
                     EPIYTVKDAKVGMFRDIRYTDYPLRSANSVGGRMTR"
     gene            complement(920..1948)
                     /locus_tag="PLT35_05085"
     CDS             complement(920..1948)
                     /locus_tag="PLT35_05085"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOG28634.1"
                     /translation="MSTLHRGDTALGEVFIHGVGHFHPENRIDNAFLASLDIGTDDAW
                     IMERVGIRSRRTVLPLEYIRTTKNAQPQLAAAASMYTNAQTGAAAARMALARSGLAPS
                     DIGLVIAGGCSPQHSIPAEACRIAAELGIQATAYDVNSACSSFAVQVHQLQQMRPSAL
                     PDYVLVVNVENTTRTVDYRDRRTAVLWGDGSAAAVLSARVPAPVRVTRSVVHSDPAGW
                     NKVVIPAGGHFVQDGRAVQGFAIRKSVATITSLASHLERDRSELLFIGHQANLMMLDA
                     VRERAGISPDRHLHNVEEFGNCGAAGAPGVLSQRWDDLPPACEIALVVVGSGLTWAGL
                     LIDVRRPS"
     gene            complement(1932..2867)
                     /locus_tag="PLT35_05090"
     CDS             complement(1932..2867)
                     /locus_tag="PLT35_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG28635.1"
                     /translation="MTNRFSERQWALVLGGSSGFGLAIAKKLAAEGMSVCVAHRDRRG
                     AMDRVDREFEAVRAHGRPFVSINTDALSPAGMADVIEALRAGMQPAGRVRVLVHSIAY
                     GNLKPIVAPPARPSAGPPLARLADELGLPPAEVDRAVRRLAEAGEPAFQLLNPPDYGD
                     AVLDDEDVARTIYSMGTSLLTWVQRLHREGLFAASARVIGLTSEGNEVAWRGYAAVAA
                     AKSALESASRAIAVEFGPYGIRSNILQPGVTDTPALRVIPGSGRMKAGALLRNPLGRL
                     TTPEDVANAAFLLSLDEADWINGALIRVDGGEHVA"
     gene            complement(3123..4580)
                     /locus_tag="PLT35_05095"
     CDS             complement(3123..4580)
                     /locus_tag="PLT35_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28636.1"
                     /translation="MHRGNRAAFAGAPRCAALTALLLSAFAGAAAWPVALRAASPPID
                     GAPAPHADATPPGASARAPRIEAPAGSQPDQEPAPVGTLPRAPRGRVRLEGRAFADDD
                     GPRPALGASLLWLAWGYRHDRARLEANLRWLAERGVDFVRALGEVGGEGWEDRAIDPA
                     WPDYAAVIGGATALANRCGLRVEWTLFGGGAVRTRDAWRAAARKAVDALRPVVAGVQF
                     VEIQNEEQGPPPDLARELAAEARHALGVEVAISGTPPAMLAALYEGSAATVATVHFDR
                     RAGDDGWQSARQAWDWRNRAGAPPAFVNNEPAGIASSVRAENDPVRLASDALVTWIAG
                     GAAYVLHHGAGIHGREYTHPTAGRRRANAWEQPALADALGIIARIRSVLPGDIAAWEK
                     AENRGQPPDPVPPFRFPADLLGARPGGPRGLAGAFTAMRGPEFFTVVLGARGDAAAEE
                     VRPVTATVRELREWVERPWPGRLAGADSTYLVHGR"
     gene            4627..>7020
                     /locus_tag="PLT35_05100"
     CDS             4627..>7020
                     /locus_tag="PLT35_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG28637.1"
                     /translation="MQRPSHGFIPGFIGLALAAVLVGAGALPAAAQTAAGGDFEGTVR
                     DATGAVLPGATLVLTNVDTGVERASVTNEIGRYRIPAVPAGAYTLRVSLDGFATMQRE
                     GLALEVGQVLRVDITLPPAGVSQEVTVTAAAPIVEQGRTQTGAVVSRTEIENLPSNGR
                     DFLSFSTQIAGVTGQQMSGQGSGISFNGQRARSNNVSLDGVDANGALNGNTRQTISQE
                     AVREYQVVTSQFAPEFGRASGGLVNIVSRSGTNDLRGNAFLYVRDESLDARNAFVTAG
                     RPEFQRQNFGGTLGGPLKRGRTFFFGAFERMRRDESGVISITDANVAAINAVLAARPI
                     PGSAVTSITNGVYPITRRDSLASLKIDHTLSPKHLLAFRYTYGKSTEANAGGVGIGGL
                     ITESGGGGQRGTDQSVLLSWTNVMSSSLLGEARLQVAPRDLEQYANDPVGPRVSISGV
                     ATWGRNTNFPVILDEVTTQGSYTLSWQRGAHFLKAGADVAHIAARSSFPVSFAGSFSF
                     GSLASFAAGTPSTFTQGFGNPQIDLPDTLYAAFVQDSWTVNDRLTFVYGLRYDYDAQP
                     QRVPRDRSNPLEAPLDDGIHRDPNNVSPRAGFTWDPIGGGRTIIRGGYGRFYDKVFLL
                     VARNALLARQSVSLSGANAALQFARGAFPESDQLPAGFTLTRPSINKADPEMQIPYLD
                     QVSLGVERQIGTHWSVAINGVRNWGSALLVSNNINLGPPTILTSANAASLGVSNPSPQ
                     QIGRPYYGSTNRLDPLYNNIQMVSSSGWSSYYGVQFTVQKRLANGYSLRANYILSESK
                     "
BASE COUNT         1016 a         2540 c         2499 g          965 t
ORIGIN      
        1 gcctcgacga acagcgtcgt ccagcgtgac gggtcctcgt ccgggtagtc cagggcgaac
       61 aggtagatgc cgagcgcgac gacgcccacc tggcccatcg actcgagcag gatcacgccg
      121 ggcgtgatgg gccggcccgg gaagtggccc gcgtagaaga actcgtcggg gcggaaggtg
      181 taccgtccgg tgatgcgctg ctcgtccacc tcgaggattt cgtccacgaa gcggaacggg
      241 cgctgctggg gcagcagatc caggatctgg gccggggtca gccgttcgcc gggcatcctc
      301 agctcctcat catgcgggcg ggcacttgcg cctcaccgcg tcatccgccc gcccacggag
      361 ttggccgacc gcagcgggta gtccgtgtag cggatgtccc tgaacatgcc gaccttcgcg
      421 tccttcacgg tgtagatcgg ctcgccgtcg acgagcaccg tcccggtgcc gatggcgatc
      481 gcggagccgg agccggccag ctcggcgtag cggcggatgt ctatctcgta gcggaccagc
      541 gtgttgtagg ggcggatctg gccgaagaac tcgatctcct tcgcgcccag cgcgcggccg
      601 ccgcccttgg cgccgcggac ggcgccgtac aggccgatga gctgccagac ggcgtccacg
      661 ccgaggcagc ccggctgcac ggggtcgccc cggaagtggc actggaagaa ccaggcatcg
      721 acggcgacgt cgtactccgc gacgatgcgc ccctgcggcc cgcggtggct gatctcgacc
      781 acgcggtcca ccatgagcat cggcgggccg gggagcgagg cgaagccctc ggagggcgga
      841 tcggtgacga gggtgccgaa tgcgcacgcg agcacctcct ccttggagaa ggacgcgcgc
      901 tgctggaact cggcgtaggt catgacggcc tccgcacgtc gatgagcagg ccggcccagg
      961 tcaagcccga ccccacgaca accagcgcga tctcgcaggc gggcgggaga tcgtcccacc
     1021 gctgcgaaag cacccccggg gcgccggcgg cgccgcagtt accgaactcc tccacgttgt
     1081 gcaggtggcg atcgggggag atccccgcgc gctcgcgcac cgcgtcgagc atcatcaggt
     1141 tcgcctggtg accgatgaac agcagctcag accggtcgcg ctcgaggtgc gacgcgagcg
     1201 acgtgatggt cgccaccgac ttgcggatgg cgaagccctg gaccgcccgc ccgtcctgca
     1261 cgaagtggcc gccggccggg atcacgacct tgttccagcc cgccgggtcc gaatgcacga
     1321 cgctgcgggt gacccgcacc ggcgccggca cccgcgccga caagaccgcg gcggcgctgc
     1381 cgtcgcccca cagcacggcg gtccggcggt cgcggtaatc gacggtccgc gtcgtgttct
     1441 cgacgttgac gacgaggacg tagtcgggca gggcggaggg ccgcatctgc tggagctggt
     1501 gcacctgcac ggcgaagctg gagcacgccg agttgacgtc gtaggcggtg gcctggatgc
     1561 cgagctcggc agcaatccgg caggcctcgg ccgggatcga gtgctgggga gaacacccgc
     1621 cggcaatcac caggccgatg tccgacggcg ccaggccgga ccgcgcgagc gccatccgcg
     1681 cggcggccgc gccggtctgg gcgttcgtgt acatcgaggc cgcggccgcc agctggggct
     1741 gggcgttctt cgtcgtcctg atgtactcga gcggcaggac ggtgcggcgg gaccggatgc
     1801 ccacccgctc catgatccag gcgtcgtcgg tgccgatgtc gagcgacgcg agaaacgcgt
     1861 tgtcgattcg gttctccggg tggaagtgcc ccacgccgtg gatgaacacc tcgccgagcg
     1921 ccgtgtcgcc cctatgcaac gtgctcaccc ccgtccacgc ggatgagcgc gccgttgatc
     1981 cagtcggctt catccaggct cagcaggaac gccgcgttgg cgacgtcctc gggcgtggtc
     2041 agccgcccga ggggattgcg gaggagggcg ccggccttca tccggccgct gccggggatg
     2101 acgcgcagcg cgggggtgtc ggtcacgccg ggctggagga tgttcgatcg aatgccgtac
     2161 gggccgaatt caaccgcgat ggcccgcgac gccgactcga gcgcggattt cgccgcggcc
     2221 acggccgcgt agcctctcca cgccacctcg ttgccctcgc tcgtgaggcc gatgacccgc
     2281 gcgctcgcgg cgaacaggcc ttcccggtgg agccgctgca cccacgtcag caggctcgtg
     2341 cccatcgagt agatcgtgcg ggcgacgtcc tcgtcatcga ggaccgcgtc gccgtagtcc
     2401 ggagggttca gcagctggaa ggcgggctcg cctgcctccg ccaggcgccg cacggcccga
     2461 tccacctcgg caggcgggag cccgagctcg tccgccagcc tcgccagcgg cgggcctgct
     2521 gagggccgcg ccggcggggc gacgatgggc ttgaggttgc cgtacgcgat cgaatgcacg
     2581 agcacgcgaa cccggccggc cggctgcatg ccggcccgga gcgcctcgat gacatccgcc
     2641 atcccggcgg gcgacagggc gtcggtgttg atgctgacga acggtcggcc atgggcgcgg
     2701 acggcctcga actcgcgatc gacccgatcc atcgcgccgc ggcggtcgcg gtgcgcgacg
     2761 cagacgctca tcccttccgc ggcgagtttc ttcgcgatgg ccaggccgaa tccgctggat
     2821 ccgcccagca ccagggccca ctggcgctcc gagaaccgat tcgtcaaccc gcgccccccg
     2881 cttccccgtc cgcgccgcct ctccggcgac ggctgcggcc gtcacgcaca ccgcagccgt
     2941 tggcccggct gccgatggcc gccacgctct tcatgcggat gccgcctctc atgttgcatc
     3001 aagggaatcc aagtccgggc gacgactccg gacgaggccg cgctccggag gccggttccg
     3061 gcgcgcttcg atcgcaccgc gagccggaag ctccgccggc atcttccgcc cacgcgctct
     3121 cgtcatcgcc cgtgcacgag gtacgtggag tcggcgccgg ccaggcggcc gggccacggg
     3181 cgttccaccc actcccgcag ctcacgcacc gtcgccgtca ccggccggac ctcctccgcc
     3241 gccgcgtcgc cacgcgcgcc gagcaccacc gtgaagaact cggggccgcg catcgcggtg
     3301 aacgccccgg cgaggcccct gggtccgccc ggccgcgcgc ccaggagatc ggccggaaat
     3361 cggaaaggcg gaaccggatc cggcggctgg ccgcggttct ccgccttttc ccaggcggcg
     3421 atgtcgccgg gcagcacgct ccggatgcgg gcgatgatcc cgagcgcgtc ggcgagcgcc
     3481 ggctgctccc aggcgttggc gcgtctgcgg cccgcggtgg gatgcgtgta ctcacgtccg
     3541 tggatcccgg cgccgtggtg caggacgtac gcggcccctc ccgcgatcca ggtcacgagg
     3601 gcgtcgctcg ccaggcgaac gggatcgttc tcggcccgca cactgcttgc gatccccgcg
     3661 ggctcgttgt tgacgaacgc gggcggcgcg ccggcgcggt tccgccagtc ccacgcctgg
     3721 cgggcggatt gccagccgtc gtccccggcc cgccggtcga agtgcacggt ggccaccgtg
     3781 gccgccgagc cctcatagag cgcggccagc atggccggcg gcgtgccgct gatcgccact
     3841 tcgacgccca gcgcgtgtcg ggcctcggcg gcgagctccc tggccagatc cggcggcggg
     3901 ccctgctcct cgttctggat ttcgacgaac tggacgccgg caacgactgg ccggagcgcg
     3961 tcgacggcct ttcgcgccgc cgcccgccag gcgtcgcggg tgcggacggc gcctcctccg
     4021 aagagcgtcc actcgacgcg cagaccgcag cggttggcca gggccgtggc gccgccgatg
     4081 accgccgcgt agtccggcca cgccgggtcg atggcgcggt cctcccagcc ctcgccgccg
     4141 acctcaccga gggccctgac gaagtccact ccccgctcgg cgagccaccg caggttcgcc
     4201 tcgaggcggg cgcgatcgtg ccggtatccc cacgcgagcc agagcaggct cgctcccagc
     4261 gcgggtctcg gcccatcgtc atcggcgaag gcacggccct cgaggcgcac cctgccgcgc
     4321 ggcgcgcgcg gcagggtgcc gacaggcgcc ggctcctggt ccggctgcga cccggcgggg
     4381 gcctcgatcc gcggcgcacg tgccgaagcg ccgggcggag tcgcgtcggc atggggcgcg
     4441 ggcgcgccgt cgatcggcgg cgacgcggcg cgcagcgcca ccggccaggc ggcggcaccc
     4501 gcgaaggcgg acagcaggag cgccgtcagc gcggcgcagc gcggcgcccc ggcgaaggcg
     4561 gcgcggttgc cccggtgcat agcgaagatt gttgatcgcc ctggggcgca ggcgtattgt
     4621 tgcgccatgc agagaccctc tcacggcttc ataccgggtt tcatcggcct agcgctcgcc
     4681 gccgtgctcg tcggcgccgg agcgctcccc gccgccgcgc agaccgcggc cggcggcgac
     4741 ttcgaaggca cggtccgcga cgcgacgggc gccgtgcttc cgggggcgac gctcgtcctg
     4801 acgaacgtgg acaccggggt cgagcgggcg tcggtgacca acgagatcgg ccggtaccgc
     4861 atcccggccg tgcccgccgg cgcgtacacg ctgcgcgtgt cgctggacgg cttcgcgacc
     4921 atgcagcgcg aagggctcgc cctcgaagtg ggccaggtcc tgcgcgtgga catcacgctg
     4981 ccgccggccg gcgtgagcca ggaagtcacg gtgacggcgg ccgcgccgat cgtcgagcag
     5041 gggcggacgc agaccggggc cgtcgtgtcg cgcacggaga tcgagaacct gccgagcaac
     5101 ggccgagact tcctgagctt ctcgacccag atcgcgggcg tcacgggcca gcagatgagc
     5161 gggcagggga gcgggatcag cttcaacggg cagcgcgcgc gcagcaacaa cgtgtcgctg
     5221 gacggcgtgg acgccaacgg ggcgctgaac ggcaatacgc gccagacgat cagccaggaa
     5281 gccgtccgcg agtaccaggt ggtgacgagc cagttcgcgc cggagttcgg gcgggcgtcg
     5341 ggcgggttgg tgaacatcgt gtcgcgctcg ggcacgaacg acctccgcgg caacgcgttc
     5401 ctctacgtgc gcgacgagtc gctggacgcg cgtaacgcgt tcgtgacggc gggccgcccc
     5461 gagttccagc gccagaactt cgggggcacg ctcggagggc cgctgaagag gggacggacc
     5521 ttcttcttcg gggccttcga gcggatgcgg cgcgacgaga gcggggtgat cagcatcacc
     5581 gacgccaacg tggcggccat caacgcggtg ctggccgcgc gcccgattcc cggcagcgcg
     5641 gtcacctcga tcacgaacgg cgtgtatccc atcacgcgcc gcgacagcct cgcctcgctc
     5701 aagatcgacc acaccctcag cccgaagcac ctgctggcgt tccgctacac gtacggcaag
     5761 agcaccgagg cgaacgcggg cggcgtcggc atcggcggcc tgatcacgga gtccggcggc
     5821 ggcggacagc gcggcaccga ccagtcggtc ctgctgtcct ggacgaacgt catgtcgtcg
     5881 tcgctgctcg gggaggctcg gctgcaggtc gccccccgcg atctcgagca gtacgccaac
     5941 gaccccgtcg gcccgcgcgt ctcgatctcg ggcgtggcga cgtggggccg gaacacgaac
     6001 ttcccggtca tcctggacga ggtgacgacg cagggcagct acacgctcag ctggcagcgg
     6061 ggcgcgcact tcctgaaggc cggcgccgac gtcgcgcaca tcgccgcgag atcgtccttt
     6121 ccggtgagtt tcgcgggcag tttctccttc gggagcctgg cgtcgttcgc ggccggcacg
     6181 ccctcgacgt tcacccaggg gttcggcaat ccgcagatcg acctgcccga cacgctctac
     6241 gccgcgttcg tgcaggattc gtggacggtg aacgaccgcc tgacgttcgt ctacggtctg
     6301 cgctacgact acgacgcgca gccgcagcgg gtgccgcgcg atcgctcgaa ccccctcgag
     6361 gcgccgctgg acgacgggat ccaccgcgat ccgaacaacg tgtcgccgcg ggcgggcttc
     6421 acctgggacc ccatcggcgg gggccgcacg atcatccgcg gcggctacgg gcggttctac
     6481 gacaaggtct tcctgctcgt ggcgcgcaac gcgctcctcg cccgccagtc ggtgtcgttg
     6541 agcggggcga acgccgcgct gcagttcgcc cgcggcgcgt ttcccgagag cgaccagctt
     6601 cctgccggct tcacgctgac ccggccgagc atcaacaagg ccgatccgga gatgcagatc
     6661 ccgtacctgg accaggtcag cctgggagtc gagcgccaga tcggcaccca ctggtccgtg
     6721 gcgatcaacg gcgtgcgcaa ctggggatcg gccctgctgg tgtcgaacaa catcaacctc
     6781 ggccctccga ccatcctgac ctcggccaac gccgcgtcgc ttggcgtcag caacccgagc
     6841 ccgcagcaga tcgggcgccc ctactacgga tccactaacc gcctcgaccc gctctacaac
     6901 aacatccaga tggtgtcgtc gagcggctgg tcgtcgtact acggcgtgca gttcaccgtg
     6961 cagaagcggc tggcgaacgg ctactccctg cgggcgaact acatcctgtc cgagtcgaag
//
LOCUS       DAOSIF010000106         6986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9812_length_6986_cov_3.762949, whole genome shotgun sequence.
ACCESSION   DAOSIF010000106 DAOSIF010000000
VERSION     DAOSIF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9812_length_6986_cov_3.762949"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLT35_05105"
     CDS             complement(<1..980)
                     /locus_tag="PLT35_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOG28638.1"
                     /translation="MPLSAVLDDVQRRVLAAERELLGNIRTALARAGADDGSLADLAA
                     SIAQLDELFLLVVVGEFNSGKSAFINALLGRAVLPEGVTPTTAEITVLAHGDPGAGQL
                     MPDRVRLVTAPVDLLRDLHIVDTPGTNAVIREHEALTTRFVPRADLVLFVTSIDRPFS
                     ESERAFLERIRAWGKHVVFVVNKHDLVTRAEEVEEVRRFVLANAAKVLGAEPTLFFVS
                     ARDALRAKTGQPALWASSGFEALEGFITGTLDDAGRLRLKLLNPLGVARHVVDARRAL
                     VTGRLDLLSGDVATLDEVDRQLAQYERDLARGFEPRMAAIDNVLLEMERRG"
     gene            1158..2891
                     /locus_tag="PLT35_05110"
     CDS             1158..2891
                     /locus_tag="PLT35_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOG28639.1"
                     /translation="MLRSRALAATAVALAVAFGAAALNSASPRPLRIEVSVPAALAPA
                     PLDGRVILVLSRRGTPEPRLARMTTLDAQPMFGVDVDGLTPGRPAVIDAGTRGWPVES
                     LRDLPPGEYFVQATLNVYTTVTRADGHTLKVHLDQWEGQDYRRSPGNLVTNVEKVRID
                     PARGGTVRIAFAKKLPPLDPPADTKYVKHLKFRSDILSRWWGTDIYLGAIVLLPDGWA
                     EHPNARYPVIYNHGHFPRGFYGFREPAEGQAPAGPPQPGGQPSQDRPQAAQPAGPSFY
                     EDWVSGRLGRVIIVQIQHPTPFYDDSYAVNSANNGPYGDALWQELVPRVERTFRGIPE
                     GWARATYGGSTGGWESLAWQIFYPDRLNGVWSFCPDPVDFSSFQAIDIYRDDNAFYPN
                     SEWKRTPVRPWNRGLDDQTFLSQYDANHYEEVLGTRGRSGEQMDIFMAVFGPVGPDGY
                     PKLLYDKWTGVIDRSVAEYWREHYDLRHILERDWKALGPKLAGKIHVFMGDQDTYLLE
                     EAAFKLQRFLEGTTDPHYGGSFEIGRRQPHCYAGEPAYPGQRPEQRYLPKMIERMLMT
                     APAGADLSWRY"
     gene            2904..3812
                     /locus_tag="PLT35_05115"
     CDS             2904..3812
                     /locus_tag="PLT35_05115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOG28640.1"
                     /translation="MGRKKAADFDPDVLLLFDQYVHGAIDRREFLEKAARYAAGGVTA
                     AMLLDALNPRFAEAQQVASDDARIAAEYVPFPSPQGNAGMRGYLVRPASAEGRLPGVL
                     VVHENRGLNPHIEDIARRLALDHFVAFAPDALAPLGGYPGDEDRARQLFPQLDQAKTR
                     EDFVAAAAFLRGRPECSGTIGVVGFCYGGGIANMLATRLPDLGAAVPFYGNQPAAEDV
                     AKIRAPLLIHYAEHDPRINAGWPAYEAALKAAKVPYTAHVYPGTQHGFNNDTTPRYDE
                     AAAKLAWKRTIEFFNAHLRTQERSRS"
     gene            3809..4531
                     /locus_tag="PLT35_05120"
     CDS             3809..4531
                     /locus_tag="PLT35_05120"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOG28641.1"
                     /translation="MNRKLLAAAIIVPALAAASAGAQDRPLNAPPPGFVSLFDGKTLN
                     GWVGRQGTYSPYEQAKLTAEQLDAKRAEWNADRDLHWHVDTAKGEIVSDGHGVHLATA
                     RDYGDFEFLVDWLMVSPNGDSGIYLRSFPQVQIWDPSNPREARNGADKGSGALWNNNP
                     DNPGRFPLVKADNPIGQWNTLRVRMVGNKVWIWLNDKQTVDGQVLDNFFDRTQPVLAR
                     GPLELQTHGSEIRFRNIFIKEL"
     gene            complement(4544..5290)
                     /locus_tag="PLT35_05125"
     CDS             complement(4544..5290)
                     /locus_tag="PLT35_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28642.1"
                     /translation="MRFVAFDMEIARPVPNGRDILAARPGIACAALASEGDARARVLF
                     DPGASPDLFDPASRAMTRAGAQLVLEVLAEAAARGSTIVTWNGAGFDFRLLADETGRR
                     DECARLARASVDMMFQVLCERGHPLALDAALRGAGLPPKLGQVRLRSGEAAPIDGAAA
                     PVFWQAGEYAAVLEYCAADVMGTLALAVACQQSRRLAWLSRSGRPNEIALRRGWLTVE
                     QCLALPLPDTSWMTQPLSRDSVVGWTAPSR"
     gene            complement(5318..6112)
                     /locus_tag="PLT35_05130"
     CDS             complement(5318..6112)
                     /locus_tag="PLT35_05130"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOG28643.1"
                     /translation="MTKRARLCRLFGLTAAACAALALLSALRAAAPGSGDPDALFRDR
                     EDLGLAAQAAKIWAARLETNPADYDAACKLARAEHWIGERLARDRTRHFKAGMAAARQ
                     AIAAGPERADGYFWLGANLGSHAGAGGILAALRYLKPTRQAFETSVRIDPRFNKGTAW
                     CLLGKYYEAVPGLFGGSKTKAEAMLRRCLAVDPDSIPGHYYLAETLLGLKRADEAREA
                     LLAAIAAPVDPDFGPEARVWKRKAARLLQELEGPADRAAAAGRRPE"
     gene            6431..>6986
                     /locus_tag="PLT35_05135"
     CDS             6431..>6986
                     /locus_tag="PLT35_05135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28644.1"
                     /translation="MVHLVERYLSYSETWIHRQIRWLPHDAVSLVAERLELPGPSAFP
                     VRSMLVLAQAYPRAVRFAALLSPSGRAGAALAVPFIRRQRPHVLHAHFGYTAWRWAAV
                     SRRLGVPLVASFYGRDLAAWLRRRPKWRSRYGRLFATGTRFLCEGPAMAAALADLGCP
                     RDKIGIQRLGVDVAGIDAAPRRLRP"
BASE COUNT         1059 a         2504 c         2467 g          956 t
ORIGIN      
        1 ccccggcgct ccatctcgag caggacgttg tcgatggcgg ccatccgcgg ctcgaacccg
       61 cgggccagat cccgctcgta ctgggcgagc tggcgatcca cctcgtccag cgtcgcgacg
      121 tcgcccgaga ggagatcgag gcgcccggtg acgagcgccc gcctagcgtc cacgacgtgc
      181 cgggcgacgc ccagcggatt gagcagcttg agccgcagcc ggccggcgtc gtccagcgtg
      241 cccgtgatga acccctcaag ggcctcgaag ccgctcgagg cccagagcgc gggctgcccg
      301 gtcttcgccc gcagggcgtc gcgcgcgctc acgaagaaca gcgtcggctc cgccccgagc
      361 accttcgcgg catttgcgag gacgaagcgc cgcacctcct cgacttcctc ggcccgcgtg
      421 accaggtcgt gcttgttcac gacgaacacg acgtgcttgc cccacgcgcg gatccgctcg
      481 aggaacgcgc gctccgactc gctgaatggc cggtcgatcg acgtgacgaa gagcacgagg
      541 tccgcgcggg gcacgaagcg cgtggtgagc gcctcgtgct cccggatgac cgcgttcgtg
      601 cccggcgtgt ccacgatgtg gaggtcgcga agcagatcga cgggcgcggt gacgagccgc
      661 acgcggtccg gcatgagctg tcccgcgccg gggtcgccgt gcgccagcac cgtgatctcg
      721 gccgtcgtgg gcgtgacgcc ctctggcagc accgcgcggc ccaggagcgc gttgatgaag
      781 gcgcttttgc cggagttgaa ctcccccacc acgaccagca ggaacagctc gtcgagctgc
      841 gcgatggacg ccgccagatc ggcgagcgat ccgtcgtcgg ccccggcacg ggcaagcgcg
      901 gtccgtatgt tgccgagcag ctcgcgctcg gccgcgagga cccggcgctg gacgtcgtcg
      961 agaacggccg agagcggcat gatgaaggga gtataccgcc tgcggcggag ccggcaggcc
     1021 gcggcgccgc cgtccgggac acccgggcgg ggcgcgctca cccgctcggg tggggggaag
     1081 acgccgccgc cgcggcaagc cggagcagac cccggtggcg gtgtggtacg ctgagaggaa
     1141 cggccccgga gactcgcatg cttcgatccc gtgcccttgc tgcaacggcg gttgccctcg
     1201 ccgtcgcgtt cggcgctgcc gcgctcaatt cggcatcgcc ccgtccgctc agaatcgagg
     1261 tgtccgtccc ggcggccctc gcgccggccc ccctcgacgg ccgcgtgatc ctggtcctgt
     1321 cgcggcgcgg gacgccggag ccgcggctgg cgaggatgac gaccctcgac gcgcagccga
     1381 tgttcggcgt ggacgtcgac ggcctcacgc ccggccggcc cgccgtcatc gacgcgggca
     1441 cgcgcggctg gcccgtcgag agcctgcgcg acctcccgcc cggcgagtat ttcgtgcagg
     1501 cgacgctcaa cgtctatacg accgtgacgc gggccgacgg ccacacgctc aaggtccacc
     1561 tcgaccagtg ggagggccag gactaccggc ggtcgccggg gaacctcgtc acgaacgtcg
     1621 agaaggtgcg aatcgatccg gccaggggcg gcacagtgag gatcgcgttc gcgaagaagc
     1681 tgccgcccct cgatcccccg gcagacacga agtacgtcaa gcacctgaag ttccgcagcg
     1741 acatcctgag ccggtggtgg ggtacggaca tctacctggg cgccatcgtg ctgctgcccg
     1801 acgggtgggc ggagcacccg aacgcccgct atcccgtcat ctacaaccac ggccacttcc
     1861 cgcgggggtt ctacgggttc cgggagccgg cggaaggcca ggcgcctgcc gggcccccgc
     1921 aacccggcgg ccagccgtcc caggaccggc cgcaggcggc gcagccggcc ggcccgagtt
     1981 tctacgaaga ctgggtgtcg ggacgcctcg gccgcgtcat catcgtgcag atccagcacc
     2041 cgacgccgtt ctacgacgac tcgtacgcgg tgaactcggc gaacaacggg ccgtatggcg
     2101 acgccctctg gcaggaactc gtgccgcgcg tggagcggac gttccgcggc atccccgagg
     2161 ggtgggcgcg cgcgacctac ggcggctcga ccggaggctg ggaatcgctg gcgtggcaga
     2221 ttttctatcc cgatcgcctg aacggcgtgt ggagtttctg cccggacccg gtggatttca
     2281 gctcgttcca ggccatcgac atctaccgcg acgacaacgc cttctacccg aacagcgagt
     2341 ggaagcggac gccggtccgg ccgtggaacc gcggcctcga cgaccagacg ttcctttcgc
     2401 agtacgacgc caaccactac gaggaagtgc tcgggacccg ggggcgctcg ggcgagcaga
     2461 tggacatctt catggccgtc ttcgggccgg tgggccccga cggctacccg aagctgctct
     2521 acgacaagtg gacgggggtc atcgatcgat cggtggccga gtactggcgc gagcactacg
     2581 atctgcgcca catcctcgag cgcgactgga aggcgctcgg gccgaagctc gcgggcaaga
     2641 tccacgtgtt catgggcgat caggacacct acctcctcga ggaagccgcc ttcaagctcc
     2701 agcggttcct cgagggcacg acggacccgc actatggagg ctcgttcgag atcggccggc
     2761 ggcagccgca ctgctacgcc ggagagcccg cgtaccccgg tcagcggccc gagcagcgct
     2821 acctcccgaa gatgatcgag cggatgctga tgaccgcgcc ggccggggcg gacctgagct
     2881 ggcggtactg acggaggacg accatgggcc gcaagaaggc agccgatttc gacccggatg
     2941 tcctgctgct cttcgaccag tacgtgcacg gcgccatcga tcggcgcgag ttcctcgaga
     3001 aggcggcccg ctacgccgcc ggaggcgtga cggccgcgat gctgctcgac gccctgaacc
     3061 ccaggttcgc cgaagcgcag caggtggcga gcgacgacgc gcggatcgcg gccgagtacg
     3121 ttcccttccc gtcgccgcag gggaacgccg gcatgcgcgg ctacctggtc cggccggcct
     3181 cggcggaagg caggctgccc ggcgtgctcg tcgtgcacga gaaccgcggc ctcaacccgc
     3241 acatcgagga catcgcgcgc cggctggcgc tcgaccactt cgtcgcgttc gcgccggacg
     3301 cgctggctcc cctcggcggc taccccggcg acgaggaccg ggcgcggcag ctcttcccgc
     3361 aactcgacca ggcgaagacc cgcgaggact tcgtcgccgc cgcggcattc ctgaggggcc
     3421 gccccgagtg ctccggcacg ataggcgtcg tcggtttctg ctacggcggc gggattgcca
     3481 acatgctggc cacgcggctg ccggacctgg gcgccgcggt gccgttctac gggaaccagc
     3541 cggctgcgga ggacgtggcg aagatccggg ccccgctgct catccactac gccgagcacg
     3601 acccgcgcat caacgccggc tggccggcgt acgaggcggc gctcaaggcc gcgaaggtgc
     3661 cctacacggc gcacgtttat ccgggcaccc agcacgggtt caacaacgac acgacgccgc
     3721 gctacgacga ggctgccgcg aagctggcct ggaagcgcac catcgagttc ttcaacgcgc
     3781 acttgagaac gcaagaaagg agccgttcgt gaaccggaaa ctgctcgctg ccgccatcat
     3841 cgtgccggcg ctcgccgccg cctcggccgg cgcccaggac cgccccctca acgcgccgcc
     3901 gccaggcttc gtcagcctct tcgacggcaa gacgctcaac gggtgggtgg gacgccaggg
     3961 cacctacagc ccgtacgagc aagcgaagct caccgccgag cagcttgacg cgaagcgcgc
     4021 cgagtggaac gccgaccgcg acctgcactg gcacgtggac acggccaagg gcgagatcgt
     4081 gtcggacggc cacggggtcc acctggcgac ggcccgtgac tacggcgact tcgagttcct
     4141 cgtggactgg ctgatggtga gccccaacgg cgactcaggc atctatctgc gcagctttcc
     4201 gcaggtccag atctgggatc cctcgaatcc ccgcgaggcc aggaacgggg ccgacaaggg
     4261 gtcgggcgcg ctctggaaca acaacccgga caatcccggg cggttcccgc tcgtcaaagc
     4321 ggacaatcca atcggccagt ggaacacgct gcgggtgagg atggtcggga acaaggtctg
     4381 gatctggctg aacgacaagc agaccgtgga cggccaggtc ctggacaatt tcttcgatcg
     4441 gacgcagccg gtcctcgcca ggggcccgct cgagctccag acgcacggct ccgagatccg
     4501 cttccgcaac atcttcatca aggagctgta gccggcccgc gcgctaccgg gacggcgccg
     4561 tccatccgac gacgctgtcc cggctgagcg gctgggtcat ccagctcgtg tccggcaggg
     4621 gcagcgccag gcactgctcc accgtgagcc agccgcgcct cagggcgatc tcgttgggtc
     4681 tgccgctccg gctgagccac gccaggcgcc ggctctgctg gcaggcgacg gcgagggcca
     4741 gcgtgcccat cacgtcggcc gcgcagtact ccaggacggc ggcgtactcc cccgcctgcc
     4801 agaagaccgg cgcggccgcg ccgtcgatcg gggccgcctc gccgctgcgc agcctgacct
     4861 ggccgagctt ggggggcagg cccgcgccgc gaagcgcggc gtcgagggcc agcggatggc
     4921 cgcgctcgca gagcacctgg aacatcatgt ccacgctcgc gcgggcgagg cgcgcgcact
     4981 cgtcgcgccg ccccgtctcg tcggcgagga gccggaaatc gaagcccgcg ccgttccacg
     5041 tcacgatcgt ggagccccgc gccgccgcct ccgcgagcac ctcgaggacg agctgcgccc
     5101 ccgcgcgggt catcgcgcgc gaagccggat cgaacaggtc cggcgacgcg ccgggatcga
     5161 agagcacccg ggcgcgagca tccccttcgg atgcgagcgc cgcgcatgcg atgccggggc
     5221 gggcggccag gatgtcccgc ccgttgggca cgggcctggc aatctccatg tcgaatgcga
     5281 caaaccgcat gagtccctcg agaggcgatg cgcccgttca ctcgggccga cgccctgcag
     5341 ccgcggcgcg gtcagcaggg ccttcgagct cctgcagcaa tcgcgccgcc ttgcgcttcc
     5401 acacgcgggc ttccggtccg aagtccggat cgacgggcgc cgcgatcgcg gccagaaggg
     5461 cctcgcgagc ttcgtctgcc ctcttgaggc ccagcagggt ctccgcgagg tagtagtgcc
     5521 ccgggatgct gtccggatcg accgccaggc agcgccgcag catagcctcg gccttcgtct
     5581 tgctgccccc gaacagcccg ggcacggcct catagtactt gccaagcagg caccacgccg
     5641 tgcccttatt gaagcgcgga tcgatgcgga cgctcgtctc gaacgcctgg cgcgtcggct
     5701 tcaggtaccg gagggccgcg aggatcccgc ctgcgccggc gtgggatccc aggttcgcgc
     5761 cgagccagaa gtagccgtcg gcacgttccg ggccggccgc aatcgcctgg cgcgccgctg
     5821 ccatgccggc cttgaaatgc cgggtgcgat cgcgggccag ccgttcgccg atccagtgct
     5881 cggcccgcgc gagcttgcag gcggcatcgt agtcagcggg gttcgtctcg agcctggctg
     5941 cccagatctt ggcggcctgc gccgccaggc cgaggtcttc gcggtcgcga aacaaggcgt
     6001 cggggtcgcc ggagcccggt gcggccgccc gaagcgccga gaggagggca agcgccgcac
     6061 aggcagccgc cgtgaggccg aacagccgac agaggcgtgc gcgcttcgtc atgacgcgtg
     6121 gtggggggcc ggcgcggcgc ccccgccgcg cctcacgccc tgtcctgatt gtaatcgagc
     6181 cggaccgaag ccggccggcg cgcgacgccc ggctcgcgac gaccggccgc gcgtcgcgga
     6241 ggacgaccgc ggagaccagc ggttgattcc gcgggagcgg cattgtacgg tggattcgcc
     6301 tgcggggatg gccggtggcg ggcgtgcggc gtccggaagc cgatcgcgga cgtgacgggg
     6361 cggaacgaat gcgatgagcg gggcggcagg acatgaaggc gacggggcac ccgggtcgca
     6421 gccgctgcgc gtggtgcacc tcgttgagcg ctacctgtcc tacagcgaga cgtggattca
     6481 ccgtcagatc agatggctcc cgcacgacgc ggtctcgctc gtcgccgagc ggctggagct
     6541 tccggggccg tccgccttcc ccgtgcgatc catgctcgtc ctcgcgcagg cgtatccacg
     6601 ggccgtccga ttcgcggcgc tcctgtcgcc gagcggccga gccggcgcgg cgctggcggt
     6661 tcccttcatc cggcgccagc gcccgcacgt gctgcacgcg catttcgggt acacggcctg
     6721 gcgatgggca gcggtcagcc ggcgcctggg cgtgcccctc gtcgcgtcgt tctacggccg
     6781 cgacctggca gcgtggctcc ggcgacgtcc gaagtggcgg agccgctacg ggcgactgtt
     6841 cgcgactggc acccgcttcc tgtgcgaggg cccggcgatg gcggcggccc tcgcggatct
     6901 cgggtgtccg cgagacaaga tcggcatcca gcggctcggc gtcgatgtcg ccggcatcga
     6961 tgccgcccct cggcgtctcc ggcccg
//
LOCUS       DAOSIF010000107         6969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_9847_length_6969_cov_3.926671, whole genome shotgun sequence.
ACCESSION   DAOSIF010000107 DAOSIF010000000
VERSION     DAOSIF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9847_length_6969_cov_3.926671"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1629
                     /locus_tag="PLT35_05140"
     CDS             70..1629
                     /locus_tag="PLT35_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28645.1"
                     /translation="MTRADRLGAGGLLLALAWLAANPGPVLLAAPVKGLTHAPLLSRA
                     FDLVYDADFAGAERALAAACGPAPPQACDVVRAAGAWWRLYLDFDDRSRDAALLTEVA
                     GVIARGEAWVRREPERAEAWLYLGAAYGVRVQYHGQRLEVLAAARDGKRIKNALERAL
                     ALDPDLHDAHAGIGLYRYYAGMAPSIFKILRWFLGLPGGNREAGIAQLRRAREHGVLM
                     RSEAAYQIYFADIWYENKPDEALELLAELRARHPGNPMFLLNTAQVHEIYRQDIPAAL
                     AAYRALVDGARRGSLHEAVLAETWGHLGAASQLQALAEQDRAIEEARAVVALRPERPY
                     GAVALAHLEAARAADALGRREEAVASYRAAQEAAPLGDPRLVRRAAQRGLQRAPDAGT
                     AEAARRSLDGWRAFERGAFEEALAELDRAVAARGQDGVHRYRRARVLAATGDRARARE
                     DFQRALDSRPPPPPPFVAASYAGLGALAEAASDPARAAAMYGAALRVRGAFPDTQDQA
                     RRALQRIRSPR"
     gene            2063..2221
                     /locus_tag="PLT35_05145"
     CDS             2063..2221
                     /locus_tag="PLT35_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28646.1"
                     /translation="MAKTVAKKAAVKKAAPKKAAVKKAAAKKAAPKKAAVKKAAAKKP
                     AKKGCKAC"
     gene            complement(2308..4455)
                     /locus_tag="PLT35_05150"
     CDS             complement(2308..4455)
                     /locus_tag="PLT35_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG28647.1"
                     /translation="MIRASARALALVALTAAHATAQTPGAGPTFDEKMAWMIRLEDQR
                     ILRAPEPLPAAPAPQPKRRLWQRRPPPPPPAAVPDLLALLRDPEGRVRRRAAQAVGRV
                     GLPEGAAPLAALLASDADAEVRQMAAFALGLIRDRSSVGPLRAALDDPSPLVRGRAAE
                     SLGLLGDAGSAPAIAAMASAVLAAGGLAGVADDDVSAGVPPLADAFRLAVVALARLKA
                     ADDLLGVVLGSSGGLRVRWWPAAFALSRIDDARAEPALEAIARDGGSVSKAFAARGLG
                     ARRARSAVAALLRLVEARAAEPRAAVSAVRALGQIGDAQAAPALRSLLRDQDVDSNLK
                     LEAIAALGALKDGPSLDTLVDLVGDAWPAARAAALRAIREIDAETYLVVLSGLDADPH
                     PSVRRAIVAALPSLGAEVAAPRLSALLAGEDVQVLPAAIEAAASLEARPPGFGEVLLR
                     LLGHADVMVRAAAASAIGAVKPEGGERALASAYRAAAGDGLCQARVAALSALAKYGAG
                     AALPVLRDALGDRDWAVRLSAAALLRAVEPASDTASRIRPAPGRGDAVDYAAPALVAP
                     AVSPHVFLDTDAGTVEIELAVLDAPLTSASFLALARSGYFSGMAIHRVVPDFVVQAGD
                     PRGDGEGGPGYTLRDEINMLPYLRGTVGMALDGPDTGGSQFFITHSPQPHLDAKYTVF
                     GRVVAGMEAVDRLRQWDVIRRVRVWDGVELVTR"
     gene            4549..5187
                     /locus_tag="PLT35_05155"
     CDS             4549..5187
                     /locus_tag="PLT35_05155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOG28648.1"
                     /translation="MDYRYLAIEGPVGAGKTALAERLALRVDATSVLEDPDNPFLADF
                     YQDKPGAAFQTQLFFLLSRHRQLAGLRQTDLFSQVTVCDYLFDRDRIYAYVSLDDNEL
                     YIYQRLYDLLVRDIPPPDLVVYLQAPTDLLLKRLRERTRDPERQAHRPDPGYVKELNE
                     AYQHFFFHYSATPLLIVETSQIDFGRSDDAVDDLLRQIRAMGRGTQYYVPRK"
     gene            5349..6197
                     /gene="accD"
                     /locus_tag="PLT35_05160"
     CDS             5349..6197
                     /gene="accD"
                     /locus_tag="PLT35_05160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOG28649.1"
                     /translation="MAWFKKEKTPIAAGSKAAARVPEGLWIKCPSCGKLIYHKDLEAS
                     LRVCPSCGHHFRMTAAARLRSLFDDAWQEHDAGLASTDPLRFADTKPYRERLRASQAA
                     TGLLDAVIVGSGTLEGIPVVIAAMEYGFIGGSMGVVVGEKITRAVERGLASRAPVIVV
                     SSSGGARMMEGALSLMQMAKVSAALARLDRAGVPFVSILTDPTTGGVTASFAMLGDLN
                     IAEPKALIGFAGPRVIEQTIRQKLPEGFQRSEFLLAHGMIDLIADRRELKATLARALR
                     FMHDAA"
     gene            6184..>6969
                     /locus_tag="PLT35_05165"
     CDS             6184..>6969
                     /locus_tag="PLT35_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG28650.1"
                     /translation="MTPLEYLFGLEFHGRKFGLENILAITDALGRPQDACPSAAVAGT
                     NGKGSVCAFVSAALTAAGHRTGCYTSPHLVRVEERFTVDGEPVGEAELAGVVEDLRAL
                     VSRLRAEGRLSASPTFFEVATAAAFELFRRRRVEVAVLEVGLGGRLDATTAAAPLAGA
                     ITNIDLDHQRDLGHTLAEIAREKAGIIKPGMRLVCGERRPEPYSVIAAACAERGATLV
                     RAWDGVTAAASGAGGRLIVELRTPARRYGPLALALRGRHQVENA"
BASE COUNT          980 a         2531 c         2533 g          925 t
ORIGIN      
        1 gctcgaccgg ttgatcgatc tggcgctcga gcgccacgcg gccaaacaga acctgcgcac
       61 gagcgcgtga tgacgcgggc agatcggctc ggtgccggcg gcctgctact ggccctggct
      121 tggctggccg cgaaccccgg gccggtgctc ctggccgcgc cggtgaaagg gctgacgcac
      181 gccccgttgc tgtcccgggc attcgatctc gtctacgacg cggacttcgc cggggccgag
      241 cgggcgctcg ccgcggcgtg cggtccggcg ccgccgcagg cctgcgacgt cgtccgggcg
      301 gcgggggcct ggtggcgtct ctacctggac ttcgacgacc gctcgcgcga cgcggcgctg
      361 ctgacggagg tggccggggt catcgcgcgg ggcgaggcct gggtgcggcg ggaacccgaa
      421 cgcgccgagg cctggctcta cctgggcgcg gcctacgggg tgcgcgtgca gtaccacggc
      481 cagcggctcg aagtcctggc ggcggcgcgc gacggcaagc gcatcaagaa cgccctggag
      541 cgcgcgcttg cgctggaccc ggacctccac gacgcacacg ccggcatcgg gctctaccgc
      601 tactacgcgg ggatggcgcc ttcgatcttc aagatcctgc ggtggttcct aggtctgccc
      661 gggggcaacc gcgaggcagg gatcgcgcag ctgcgtcgcg cacgggaaca cggcgtgctg
      721 atgcgctccg aggccgccta ccagatctac ttcgcggaca tctggtacga gaacaagccc
      781 gacgaggcgc tggagctcct ggccgagttg cgcgcgcggc acccgggcaa cccgatgttc
      841 ctgctcaaca cggcccaggt gcacgagatc taccgccagg acatcccggc cgcgctcgcg
      901 gcgtaccgcg cgcttgtgga cggcgcgcgg cgagggtcgc tgcacgaagc ggtgctggcc
      961 gagacgtggg gccacctggg ggcggcctcg cagctgcagg ccctggcaga acaggaccgg
     1021 gcgatcgagg aggcgcgggc cgtggtcgcg ctgcggcccg agcggccgta cggggccgtc
     1081 gcgctggcgc acctcgaggc ggcgcgcgcc gcggatgcgc tcggacggcg cgaggaggcg
     1141 gtcgcgtcgt accgcgcggc gcaagaggcg gcgccgctcg gcgatccccg cctcgtccgg
     1201 cgcgcggcgc agcgcgggct ccagcgcgcg cccgatgccg gcaccgccga ggcggcccgg
     1261 cggtcgctgg acgggtggcg cgccttcgag cgcggcgcct tcgaggaggc gctggcggag
     1321 ctggatcgcg cggtggccgc gcgcgggcag gacggcgtgc accgttaccg gcgcgcccgc
     1381 gtgctcgcgg cgacaggaga ccgcgcgcgg gcccgggagg acttccagcg ggcgctcgac
     1441 tcgcggccgc cgcctccgcc gcctttcgtc gccgcgagct acgccggcct cggcgcactc
     1501 gcggaggccg cgtccgatcc cgccagggcc gccgcgatgt acggcgccgc gctgcgcgtg
     1561 cgcggcgcct tccccgacac tcaggaccag gcgcgccgcg ccctccagcg catccggtcg
     1621 ccgcgctgac gccgccgcgc ggcggcgctt gcacggcaac caccgccgcg gatggcacgg
     1681 gcgcccgccc gcggtcgcgt ggagatcacc cgggcccgcg acggcagtcg cgttcgcgat
     1741 cactttgtgc ttgacatcgt ttcgttctca tccataatct acagcttgta tgagcctgga
     1801 ttgctcagcc cagtgcttgt gatcgaccgg cggcagtaac cagctgagcg gccacttccc
     1861 gaggaggaac aatggcgaag aagactgcga agaaggccac gaagaaggct gcgaagaagc
     1921 gctaggcttc gacgcgccga tgacgggggt gcgattcacc cccgttcttt tttttcagcc
     1981 cgcacacgcc cgctcatccg ggcatcatca taaagccggc cggcgctccc cgccggccgg
     2041 acgtgcgagg gaggagccga tcatggcgaa gactgttgcg aagaaggcgg ccgtcaagaa
     2101 ggccgcgccg aagaaggcgg ccgtcaagaa ggccgccgcc aagaaggccg cgccgaagaa
     2161 ggcggccgtc aagaaagccg ccgccaagaa accggcgaag aagggctgca aggcctgctg
     2221 acgccccggc gcgtcggcga cggcgggcgc gcgccgagcg cgcccgtttt tttttgtgtt
     2281 ccagcccccg ccgcggcgcc gctccgctca gcgcgtcacc agctccacgc catcccagac
     2341 cctgacccgg cggatcacat cccactggcg cagccggtcc accgcctcca tgcctgcgac
     2401 aacccgcccg aacaccgtgt acttcgcgtc gaggtgcggc tgcggcgaat gggtgatgaa
     2461 gaactggctg ccgcccgtgt ccgggccgtc cagagccatc ccgaccgtgc cgcgcaggta
     2521 cgggagcatg ttgatctcgt cgcggagcgt gtagcccgga cctccctccc cgtcgccgcg
     2581 cgggtcgccc gcctggacca cgaagtccgg caccacgcgg tgaatcgcca tcccgctgaa
     2641 gtagccgctt cgcgccagcg cgaggaagct cgccgacgtg agcggcgcgt cgagcacggc
     2701 cagctcgatt tcgacggtcc ccgcgtcggt gtcgaggaac acgtgaggcg acacggccgg
     2761 ggccaccagg gcgggggcgg cgtagtccac ggcgtccccc cgccccggcg cgggcctgat
     2821 ccgcgaggcg gtgtccgagg ccggctcgac ggcccgcagc agtgcggcgg cgctgaggcg
     2881 gacggcccag tctcgatcgc cgagcgcgtc gcgcaacacg ggcagcgccg cgccggcgcc
     2941 gtacttcgcc agggcggaga gcgccgccac gcgggcctgg cacaggccgt cgccggccgc
     3001 cgcgcggtac gcggaggcga gcgcgcgctc tccgccctcg ggtttcacag cgccgatggc
     3061 cgacgccgcg gcggcgcgca ccatcacgtc cgcgtggccc agcagccgca gcaggacctc
     3121 gccgaagccc ggcggcctgg cctcgagcga cgcggcggcc tcgatggccg cgggcagcac
     3181 ctggacatct tcgccggcga gcagcgccga cagccgcggg gcggccacct cggcgcccag
     3241 cgacggcagc gccgcgacga tggcccggcg cacgcttggg tgcgggtctg cgtcgaggcc
     3301 ggagagcacg acgagatacg tctcggcgtc gatctcgcgg atcgcccgga gcgcggccgc
     3361 tctggcggcg ggccacgcgt cgccgaccag gtcgaccagt gtgtcgagcg acggcccgtc
     3421 cttcagcgcg ccgagcgcgg caattgcctc gagcttcagg ttcgaatcga cgtcctgatc
     3481 gcgcaggagc gaacgcaacg cgggggcggc ctgcgcgtcg ccgatctgcc cgagcgcccg
     3541 cacggcgctg actgccgcgc gcggctcggc cgctctggcc tcgacgaggc gcaggagcgc
     3601 agccacggcc gagcgggccc tgcgcgcgcc gagtcctctc gccgcgaacg ccttcgagac
     3661 gctgccgccg tcgcgcgcga tcgcctccag cgccggctcg gcccgcgcat cgtcaatgcg
     3721 cgacagcgcg aaggcggcgg gccaccaccg cacgcggagc ccgcccgaag agccgagcac
     3781 gacacccagc agatcgtcgg cggccttgag ccgcgccaag gcgacgaccg caaggcgaaa
     3841 cgcgtcggcc agcggaggaa ccccagccga gacatcgtcg tcggcgacgc cggccaggcc
     3901 gcccgcggcc agcacggccg acgccattgc ggcgatggcc ggcgcggacc cggcgtcgcc
     3961 gaggaggccc agcgattcgg cggcgcgccc ccgcacaagc ggcgagggat cgtcgagcgc
     4021 ggcgcggagc ggcccgaccg acgagcggtc gcggatgagg cccagggcga acgccgccat
     4081 ctgccggacc tcggcatcgg cgtccgacgc gaggagcgcg gcaaggggcg cggcgccttc
     4141 cgggagcccg acgcgaccga cggcctgcgc cgcccgccgg cgcacgcgcc cttcgggatc
     4201 gcggagcaga gccagcaggt ccggaaccgc cgccggcggc ggcggcggcg gccggcgctg
     4261 ccagagccgc cgcttcggct gcggcgcagg agcggcgggg agaggctcgg gtgcgcgcaa
     4321 gatccgctgg tcctcgaggc ggatcatcca ggccatcttc tcgtcgaagg tcggcccggc
     4381 cccaggcgtc tgcgccgtcg cgtgggccgc cgtcagcgcc accagggcga gcgcccgcgc
     4441 gctcgcgcga atcatcggcc cggcctctct tgacagcctc ccctcgtcac accgccatct
     4501 taccgcgcct tgccctggcc gcgcggcggt cggtatactc gccgcacctt ggattaccgc
     4561 tacctggcga ttgaggggcc ggtcggggcg ggcaaaacgg cgctggccga gcgcctcgcc
     4621 ctgcgcgtcg acgccacgtc ggtgctggag gatcccgaca acccgtttct cgccgacttc
     4681 taccaggaca agcccggcgc cgcctttcag acgcagctgt tcttcctgct gagccgtcac
     4741 cgccagctcg ccgggctgcg gcagaccgac ttgttcagcc aggtgaccgt ctgcgactat
     4801 ctcttcgatc gggaccggat ctacgcgtac gtgagcctgg acgacaacga gttgtacatc
     4861 taccagcggc tgtacgatct cctcgtccgg gacatcccgc cgcccgatct cgtggtctac
     4921 ctgcaggcgc cgaccgacct gctgctcaag cgcctccggg agcgcacgcg agacccggag
     4981 cggcaggccc accgccccga tccgggctac gtcaaggagc tcaacgaagc gtatcagcac
     5041 ttcttcttcc actacagcgc gaccccgctc ctcatcgtcg agacctcgca aatcgacttc
     5101 ggacgcagtg acgacgccgt ggacgacctg ctgcggcaga tccgcgcgat gggccgcggc
     5161 actcagtact acgtgccccg gaagtagcgg gcaacggggt cagatccagg attccggggt
     5221 caggtcgacg gtctcggcac cgcgcctgac cgtgctgccg tccgcgaggc attcgggatc
     5281 ccggctttcg atctcgtgag cctcgagttc tggattccca tttccgcctt ctgatagagt
     5341 tcctgaaagt ggcctggttc aagaaggaga agaccccgat cgccgcgggg tcgaaggcgg
     5401 ctgcgcgtgt ccccgagggc ctgtggatca agtgcccctc gtgcggcaag ctcatctacc
     5461 acaaggatct cgaggcgagt cttcgcgtct gcccgtcctg cggccaccat ttccgcatga
     5521 cggcggcggc gcggctgcgg tcgttgtttg acgacgcgtg gcaggagcac gatgccggcc
     5581 tggcatcgac cgatcccctg cgctttgccg acacgaagcc ctatcgcgag cggctgcggg
     5641 ccagccaggc ggccacgggc ctgctcgacg cggtcatcgt cggcagcggc acgctggagg
     5701 ggatcccggt cgtcatcgcc gcgatggagt acggtttcat cggcgggagc atgggcgtgg
     5761 tcgtcggcga gaagattacg cgggccgtcg agcgcggcct ggcctcgcgc gcgccagtga
     5821 tcgtcgtgtc ctcgtccggg ggcgcgcgaa tgatggaggg cgccctgtcg ctgatgcaga
     5881 tggcgaaggt gtcggcggcc ctcgcgcggc tggaccgcgc cggcgtgccg tttgtgtcga
     5941 tcttgaccga cccgacgacg ggcggcgtca ccgccagttt cgcgatgctg ggggatctga
     6001 acatcgcgga gccgaaggcg ctcatcggct tcgcggggcc gcgcgtcatc gagcagacga
     6061 tccgccagaa gctgccggag ggcttccagc gcagcgagtt cctgctggcc cacggcatga
     6121 tcgacctcat cgccgaccgg cgcgaactga aagcgacgct cgcccgggcc ctccgtttca
     6181 tgcatgacgc cgcttgagta cctgttcggg ctcgagtttc acggccggaa gttcggcctc
     6241 gaaaacatcc tggcgatcac ggacgccctt gggagaccgc aggatgcctg tccgtcggcg
     6301 gcggtggcgg gcacgaacgg caaggggtcc gtgtgcgcgt tcgtctcggc agccctgacg
     6361 gcggccgggc accggacggg gtgctacacg tcgccccacc ttgtccgagt cgaggagcgg
     6421 ttcaccgtgg acggcgagcc cgtcggcgag gccgagttgg ccggcgtcgt cgaggacctg
     6481 cgcgcgctcg tctcgcgttt gcgcgcggag ggccgcctgt ccgcctcccc caccttcttc
     6541 gaagtcgcga cggcggccgc cttcgagctg ttccgccggc ggcgcgtcga agtcgcggtc
     6601 ctcgaagtgg ggcttggcgg gagactggac gccacgacgg cggccgcacc gctggccggc
     6661 gcgatcacga acatcgacct cgatcaccag cgcgatctcg ggcacaccct ggccgagatc
     6721 gcccgcgaga aggcggggat catcaagccc gggatgcggc tcgtctgcgg cgagcgcagg
     6781 cccgaaccgt actcggtcat tgcggccgcg tgcgcagagc ggggtgctac gctcgtccgg
     6841 gcctgggacg gcgtgacggc ggccgcctcc ggggccggcg gccggctaat cgtcgagctc
     6901 cgcacgccgg cccgccggta cggcccattg gcgctggcgc tccgcgggcg ccaccaggtg
     6961 gagaatgcg
//
LOCUS       DAOSIF010000108         6874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10026_length_6874_cov_4.574718, whole genome shotgun sequence.
ACCESSION   DAOSIF010000108 DAOSIF010000000
VERSION     DAOSIF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10026_length_6874_cov_4.574718"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="PLT35_05170"
     CDS             <1..1626
                     /locus_tag="PLT35_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28651.1"
                     /translation="VTWSCETGGGIHKTTDGGRTWVPINAGLPEAKFRGRIGLDVSRS
                     APNVLYALVDNYELSREPTEEERADPYGLPSSGFIKGATVYRSNDKGATWAQVSGLTP
                     EQKAFMERHSNTYGWVFGQVRVDPTDAETVYTMGLGLNVSTDGGRTFRQLRTPGIDHH
                     ALWIDPDNPSYLVNAYDQGLAISYDRGATWKDTRLTLPVAQFFNVSYDMSTPFRVYGS
                     MQDHGSFRAAVDLSRGRDRVPAADFERAPGGEGSTHAIDPDDPDIVYSSGFYGTLTRS
                     DLAKPAGSPQRSKSLLPARYPDEPRLRGEWLAPTIVSPHNGRTIYHGMQYLMRSRDRG
                     DTWDIISPDLTSHTGSETGDIPYHTITAISESPLRAGLIYVGTDDGKVHVTRDGGASW
                     TEILAGLPHEKWVSRIVASAFDLATVYLTQSGKRDDDFTPYVWKSADFGRTWVSLAGD
                     VPIGPVNVIREDPSDRNILYLGTDLSVYATADGGTSWQPLGGNLPSTYVLDLVIHPRD
                     NVVVIATHGRGMWVLDAEPVNRRAARPRRPAGS"
     gene            1727..3223
                     /locus_tag="PLT35_05175"
     CDS             1727..3223
                     /locus_tag="PLT35_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28652.1"
                     /translation="MAPLLLRSLYLKVVAPIVALVLAAMAIVSYLAMTAMGEGVRVAA
                     EQRARYGVAFTRSSVEDVEHAMLAKRAGSLQNLLERLGRSPDLDAIRILSPSGQVLYS
                     SKRAEVGLMMPAHLPEGPVSGDADNATVVEKRLPGILHAGSPLYNHDRCTPCHEGSRR
                     ILAYVDVDISLSRQSAGMRSWDDLAARTALALFVLLGAGVVLTLGIVVVGPIRRLSAR
                     MKEVQRGDLDVAADPSGTAEIDQLVTGFNEMVARLRQARQAEEEAQRSRMARAEQLAT
                     LGEIAASLAHEIRNPLAGAKAAIDVLAGEEREAEPRRILRSVSEELSRVDGVVRQLLG
                     FARPKAPVLGRVDLRPIIDDVVTLSRPMTAAQGVALDVQCGPEPIEALADADMVRQVV
                     LNLLLNAVQASEGISGATVSIAAILRGGQVVCSVRDQGPGIPADRAETIFRPFTTTKA
                     RGTGLGLATSRRLVELQGGRLWLDNPGAPCACFVFTLPPFAGLADAEA"
     gene            3228..4601
                     /locus_tag="PLT35_05180"
     CDS             3228..4601
                     /locus_tag="PLT35_05180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG28653.1"
                     /translation="MVRDRILVIDDDQLQRWAMGRQLTTWGYEVLEAADGQSGLDAFA
                     RHLPDLVLLDLQLPDQNGVEVLRQIRAIDPQAVVIMVTAHGGVPDAVAAFKLGVFDFL
                     PKPVDFDALGVTLRYGLEARRLREEVVRLRSGDQELADTTIVGESPAIAACVRMLDRL
                     AASDAGAVLLTGESGTGKDLFARTLHRRSSRADHPFVAVNCAAIPDTLMESELFGHEK
                     GAFTDARFLKKGVFELADGGTLYLDEVGELKPSLQPKILRVLESLTFRRVGGSRDITV
                     DLRVIAATNRDLDAAVRQGEFRRDLLYRLRVIELAIPPLRERRQDIPPLARHFLRQLA
                     LKFHKPVLGVSDAAMDVMTQYDWPGNARELKNAIERAVILEDGEQITPSGLPCQLTPP
                     AGGRASGPPGEFTLPIGGVSLERVEESLVRQAMAAAGGNQTHAARLLDISRDALRTRL
                     KKFGIER"
     gene            4764..5162
                     /locus_tag="PLT35_05185"
     CDS             4764..5162
                     /locus_tag="PLT35_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28654.1"
                     /translation="MITRRALALVAEDTELGRWALTRALETAGFDVQAVATWSEAAAW
                     LSRASFSLAFVAVSSVSGSAADVAASMRAYHPRTRLVLLADQDRADELRLACGPGQVL
                     LTKPLDLEKVASVAHLGLAADGMAGRRGNP"
     gene            5198..6184
                     /locus_tag="PLT35_05190"
     CDS             5198..6184
                     /locus_tag="PLT35_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28655.1"
                     /translation="MTEIRARRHGTLASGRRSPRRDAARCAAAAAWILVLSGSAALAQ
                     APAAYLYSLSSFSGPLRDDGARVRVDAERDEVLVIYQNTVRVFNRSGMEFYSFGADLD
                     LGQILDLAVEPGGDIVLLSYKDSRSLVTRCNFRGEPLGPIEIGGLPEGLAFHANRMVL
                     RNERFYFASLAASSVIVTDAKGTFLRHVDLPSMMQPEERQRGGTEALGFSVDEEGSIF
                     LTLPSVFRVYKIAEDGGLSEFGRPGAVPGRFGIVAGIAVDRRGNLLVADKLKCVVMLF
                     DKDFGFVGEFGYRGPAPENLIVPEDVAVDRLDRVYVAQGRRRGISVFALASR"
     gene            6425..>6874
                     /locus_tag="PLT35_05195"
     CDS             6425..>6874
                     /locus_tag="PLT35_05195"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOG28656.1"
                     /translation="MKALKIGLAVLAGAALLLLASPAPEAFHSGGVAECGGCHSMHSP
                     KPGGNYLLVGTDGASTCLSCHMHAGDTGPNGYHIATAESDMPAGVPPLQRTPGGDFGW
                     LKKTYTFTVRGTTTVEQGETHGHNIVAADTSFVADTVNTTAPGGTFPS"
BASE COUNT         1024 a         2357 c         2475 g         1018 t
ORIGIN      
        1 gtgacgtgga gctgcgagac cggcgggggg atccacaaga ccaccgacgg cggccggacg
       61 tgggtgccga tcaacgcggg cctgcccgag gcgaagttcc ggggccgcat cggactcgac
      121 gtttcccgat ccgcgccgaa cgtgctgtac gccctcgtgg acaactacga gttgtcccgc
      181 gagcccaccg aggaggagag ggccgacccg tacggcctcc cgtcgtccgg cttcatcaag
      241 ggcgccaccg tgtaccgctc gaacgacaag ggcgcgacgt gggcccaggt gagcggcctg
      301 acgccggagc agaaggcgtt catggagcgc cactcgaaca cctacggatg ggtcttcggg
      361 caggtgcgcg tcgatccgac cgacgcggaa acggtgtaca cgatgggcct cggcctgaac
      421 gtgtcgaccg acggcggcag gacgttccgg cagctccgga ccccgggcat cgaccaccat
      481 gccctgtgga tcgaccccga caatccgagc tacctggtca acgcgtacga ccaggggctg
      541 gccatctcct acgatcgcgg ggcgacctgg aaggacaccc ggctcacgct gccggtggcg
      601 cagttcttca acgtgtcgta cgacatgagc acgccgttcc gggtctatgg gtcgatgcag
      661 gaccacggga gcttccgcgc cgccgtggat ctcagccgcg ggcgcgaccg cgttcccgcg
      721 gcggacttcg agcgggcgcc cggcggcgag ggctcgacgc acgcgattga tcccgacgac
      781 ccggacatcg tgtattcgtc cggcttctac ggcacgctga cccggagcga tctcgcgaag
      841 cccgcgggca gtccgcagcg gtccaagagc ctgctgcccg cgcgctatcc ggacgagcct
      901 cgcctgcgcg gcgagtggct ggcgccgacg atcgtgtcgc cgcacaacgg ccggacgatc
      961 taccacggca tgcagtacct gatgcggtcg cgcgaccgcg gggacacgtg ggacatcatc
     1021 agcccggacc tgacgtccca cacggggtcc gagacgggcg acatcccgta ccacacgatc
     1081 acggcgatct cggagtcgcc gctgcgcgcc ggcctgattt acgtcggcac cgacgacggg
     1141 aaggtgcatg tgacgcgtga cggcggcgcc tcctggaccg agatcctggc gggcctgccg
     1201 cacgagaagt gggtctcgcg catcgtcgcg tcggccttcg acctcgccac cgtctacctg
     1261 acgcagagcg ggaagcgcga cgatgacttc acgccgtacg tgtggaagtc ggccgacttc
     1321 ggccggacct gggtcagcct cgccggcgac gtgccgatcg ggcccgtcaa tgtcatccgg
     1381 gaggacccgt cggatcggaa catcctctac ctgggcaccg atctgagcgt gtacgcgacg
     1441 gccgacgggg ggacgagctg gcagccgctc ggcggcaacc tgccttcgac ctacgtgctg
     1501 gacctcgtga tccacccgcg tgacaacgtc gtggtcatcg cgacgcacgg gcggggcatg
     1561 tgggtgctcg acgcggagcc ggtcaaccgg cgcgccgcga ggccgaggcg gccggccgga
     1621 agctaggccg tcgccgcggg cccgcgggca actgtgctag actccggccc ggcggcgagg
     1681 cgggcgcgca tcgccgcccc tgcggccccg acaagcttct gcacccatgg cgcccctgct
     1741 gctgcgcagc ctctacctca aggtcgtcgc gcccatcgtc gcgctggtgc tggcggcgat
     1801 ggcgatcgtc tcgtacctgg cgatgacggc gatgggcgaa ggcgtgcgcg tggccgccga
     1861 gcagcgcgcc cgctacggcg tcgctttcac gcggagcagc gtggaagacg tcgagcacgc
     1921 gatgctcgcc aagcgcgccg gcagcctcca gaacctgctc gagcgcctcg gccggagccc
     1981 ggatctggat gccattcgca tcctgtcgcc ctccgggcag gtgctctatt cgtcgaagag
     2041 ggccgaggtc gggctcatga tgccggcgca tctgcccgaa ggccccgtgt ccggcgatgc
     2101 cgacaacgcc acggtggtcg agaagcgcct gcccggcatc ctccacgccg gcagcccgct
     2161 gtacaaccac gaccgctgca cgccctgcca cgagggcagc cggaggattc tggcctacgt
     2221 ggacgtggac atcagtctgt cccgccagag cgccggcatg cggtcgtggg acgacctagc
     2281 ggcgaggacg gccctcgcgc tcttcgtcct gctgggggct ggagtggtgc tgacgctcgg
     2341 gattgtcgtc gtcggcccga tccgccgcct gtcggcgcgc atgaaggagg tccagcgcgg
     2401 ggacctcgat gtcgccgccg atccctcggg caccgccgag atcgatcagc tcgtgacggg
     2461 gttcaacgag atggtggcca ggctgcggca ggcgaggcag gcggaggaag aggcccagcg
     2521 cagccgcatg gcgcgtgccg agcagctcgc gacgctcgga gagatcgcgg ccagcctcgc
     2581 ccacgaaatc cgcaaccccc tcgcgggcgc caaggccgcc atcgacgtgc tggcgggcga
     2641 ggagcgcgag gccgagccga ggcgtatcct ccgaagcgtg tccgaggagc tctcgagggt
     2701 cgacggcgtc gtcaggcagc tgctcggctt cgccaggccg aaggcgccgg tgctcggcag
     2761 ggttgatctg cggccgatca tcgacgacgt ggtgacgctg tcgcggccga tgacggcggc
     2821 tcagggcgtg gccctggatg tgcaatgcgg cccggagccg atcgaggcgc tcgccgacgc
     2881 cgacatggtg cgccaggtgg tcctgaacct gcttctcaac gccgtccagg cgtcggaagg
     2941 catcagcggg gccacggtgt cgattgcggc catcctgcgg ggcggacagg tcgtctgcag
     3001 cgtgcgggac caggggcctg gcatcccggc cgaccgggcc gagacgatct tccggccgtt
     3061 cacgacgacg aaggccagag gcacgggcct cggcctcgcg acgagccggc ggctcgtcga
     3121 gctccagggc ggccggttgt ggctcgacaa ccctggcgcg ccgtgcgcct gcttcgtgtt
     3181 cacgctgccc ccgttcgccg ggctggcgga cgcggaggcc tgagcggatg gtcagggacc
     3241 gcatcctcgt catcgacgac gaccagctgc agcgctgggc catgggccgg cagctcacca
     3301 cgtggggcta cgaggtgctc gaggccgccg acgggcagtc cggcctcgac gcgttcgcgc
     3361 gtcacctgcc cgatctcgtg ctgctcgact tgcagttgcc cgaccagaac ggcgtcgagg
     3421 tgctgcgcca gatccgcgcc atcgacccgc aggcggtggt gatcatggtg acggcccacg
     3481 gcggcgtgcc cgacgccgtc gcggccttca agctcggggt gttcgacttc ctgcccaagc
     3541 ccgtcgactt cgacgcgctc ggcgtcacgc tgcgctacgg cctggaagcg cgtcgcctgc
     3601 gcgaggaggt cgtccggctg cggtccggcg accaggaact ggcggacacg accatcgtcg
     3661 gcgagtcgcc cgccatcgcg gcgtgcgtca ggatgctcga ccggctcgcc gccagcgacg
     3721 ccggcgccgt cctgctgacc ggcgagagcg gcaccgggaa ggacctcttc gcccggacgc
     3781 tgcaccgccg gagctcgcgg gcggaccacc cgttcgtggc ggtcaactgc gcggcgattc
     3841 ccgacacgct catggagtcg gagctcttcg ggcacgagaa gggcgcgttc accgacgcgc
     3901 ggtttctgaa gaagggggtg ttcgagctcg ccgacggggg caccctgtac ctcgacgaag
     3961 tcggcgaact caagccgtcg ctccagccca agatcctgcg cgtcctcgag agcctgacct
     4021 tccggcgcgt cggcgggtcg cgcgacatca ccgtcgatct gcgcgtcatc gccgccacca
     4081 accgcgatct cgacgccgcc gtccggcagg gcgaattccg gcgcgacctg ctctaccggc
     4141 tccgcgtgat cgaactggcg attccgccgc tgcgcgagcg gcggcaggac attccgcccc
     4201 tggcgcggca ttttctccgg cagctcgcgc tcaagttcca caagcccgtc ctcggcgtgt
     4261 cggacgcggc gatggacgtc atgacgcagt acgactggcc gggcaacgcc agggagctga
     4321 agaacgccat cgagcgggcg gtgatcctcg aggacggcga acagatcacc ccgagcggcc
     4381 tgccgtgcca gctgacgccc ccggccggcg ggcgcgccag cgggcccccg ggcgagttca
     4441 cgctgccgat cggcggcgtg tcgctcgaac gggtcgagga gtccctggtc cgccaggcca
     4501 tggcggcggc cgggggcaat cagacgcacg ccgcgcgcct gctcgacatc tccagggacg
     4561 ccctccgcac aaggctcaag aagttcggca tcgagcgctg agcgcgtgat ctcgcgcact
     4621 ggtgcgggat tcaccgcagg tcggcgccct catcccgccg tacgagccgc gccaggccgc
     4681 cgcttgggcg ccaccggcgg ccgaccctcc tggcacggcg attgattcac ggaacgggag
     4741 aagaccgagc caggcatccg acgatgatca ctcggcgtgc gttggcgctg gtggccgaag
     4801 acacggagct gggacgctgg gcgttgaccc gggccctcga gacggccggg ttcgacgtgc
     4861 aggccgtcgc cacctggagc gaagccgcgg cgtggctgag ccgggcgagt ttctcgctgg
     4921 cgttcgtggc ggtgtcctcc gtgtcgggaa gcgcggctga tgtggccgcc tcgatgcggg
     4981 cctaccaccc gcgcacgcgc ctcgttctgc tcgcagacca ggaccgcgct gacgagctgc
     5041 gccttgcatg cggtccgggg caagttctcc tcaccaaacc gctcgacctc gagaaggtcg
     5101 cctccgtggc gcatctcggg ctggctgctg acggcatggc gggtcggcgc ggaaaccctt
     5161 gacaggccgc gcgacccgct ggatgctgtg gaccagcatg actgagatcc gggcgcgtcg
     5221 gcacgggacg ctggcgtcgg gacggcgctc gccacgtcgg gacgccgccc gctgcgccgc
     5281 tgcggcggcc tggatcctgg tgctgtccgg cagcgccgcc ctcgcgcagg cgccggcagc
     5341 ctacctgtac agcctatcga gtttctcggg gccgctgcgc gacgacgggg cgcgggtccg
     5401 ggtcgatgcg gaacgtgacg aggtgctggt catctaccag aacacggtcc gcgtgttcaa
     5461 ccgttccggc atggagttct acagcttcgg cgcggacctg gacctgggcc agatcctcga
     5521 cctcgccgtg gagcccggcg gcgacatcgt cctgctctcg tacaaggact cgcgctcgct
     5581 ggtgacccgc tgcaacttca ggggggagcc gctcggcccg atcgagatcg gcggcctgcc
     5641 ggagggcctg gcgttccatg cgaaccggat ggtgctgcgc aacgaacggt tctacttcgc
     5701 ctcgctcgcc gcctcgagcg tcatcgtcac cgacgccaag gggacgtttc taaggcacgt
     5761 cgatctcccg tcgatgatgc agccggagga gcggcagagg ggcggaacgg aagccctcgg
     5821 attcagcgtg gacgaagagg gcagcatctt cctgacgctg ccgtcggtct tccgggtgta
     5881 caagatcgcg gaggacgggg ggctctcgga gttcgggagg ccaggtgccg tgccgggaag
     5941 gttcggcatc gtcgcgggca tcgcggtgga ccgccgcggc aacctcctgg tggccgacaa
     6001 gctgaagtgc gtggtgatgc ttttcgacaa ggacttcggc ttcgtcgggg agttcggcta
     6061 cagggggccc gctcccgaga acctcattgt tccggaggac gtggccgtcg accggctcga
     6121 ccgggtgtac gtggctcagg ggcgccgtcg gggcatcagc gtttttgcgc tggcgtcccg
     6181 gtaggcgctg gcgccgggcg gcgagggggt tccggatgcg tgaaatcacc cggcgtgagg
     6241 gatctcgcgc agctcgaggc ggtcgggtgc tgggttcgaa cgcgccggcg cggccaaaac
     6301 gattgcacgg gaagaagttg cgttggcgca cgccgcgacg ggaggttgga tccggaattg
     6361 cacgtttgtg gcatgctggt gaggcttgcc ttcggcgaag ctgtcatcat ttcggagggt
     6421 caggatgaaa gcactgaaaa tcgggttggc tgtgctggcc ggcgctgccc tgctcctgct
     6481 ggcgagcccc gcccctgagg cgttccattc cggtggcgtc gccgagtgcg gagggtgcca
     6541 cagcatgcac tcgccgaagc cggggggcaa ctacctgctc gtgggcaccg acggcgcctc
     6601 gacctgcctg tcgtgccaca tgcacgcggg cgacacggga ccgaacgggt accacatcgc
     6661 gacggccgag tccgacatgc cggcgggcgt tccgccgctg cagcggacgc ctggcggcga
     6721 cttcggctgg ctgaagaaga cctacacctt caccgtccgc ggcacgacca ccgtcgagca
     6781 gggcgagacg cacggccaca acatcgtggc ggccgacacg tccttcgtgg cggacacggt
     6841 gaacacgacg gctccaggcg gcacgttccc gtcg
//
LOCUS       DAOSIF010000109         6870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10030_length_6870_cov_3.667058, whole genome shotgun sequence.
ACCESSION   DAOSIF010000109 DAOSIF010000000
VERSION     DAOSIF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10030_length_6870_cov_3.667058"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLT35_05200"
     CDS             <1..573
                     /locus_tag="PLT35_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28657.1"
                     /translation="HEAWPAKDAKAMAALLPDIERHAAAVANAVLPGILRDKQAAWLA
                     KVTDLKAAVASYKAAVGSGDHEALFKAAEKVHAEYEGLVKIVRPVLKEMEEFHAALYV
                     LYHHQLSPFQMAKVSESVGILKLKMDALNTAVLPARLSAKADAFGLQRARLSKAVDDL
                     VAAVAGRDEVKVRAAIEQMHAEYENLERVF"
     gene            961..1185
                     /locus_tag="PLT35_05205"
     CDS             961..1185
                     /locus_tag="PLT35_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28658.1"
                     /translation="MSADVECASVSEMAVKTLACAVIKQALCDALDPTVPERIRRDAE
                     QFLAGDVRFRRWSAVAGVPPDGLLSRRLTA"
     gene            1407..3419
                     /locus_tag="PLT35_05210"
     CDS             1407..3419
                     /locus_tag="PLT35_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG28659.1"
                     /translation="MPATRTIRTVCPRNCYCTCGMLVSLDERDRIVAIEGDPENPATG
                     GRVCLKGLAYAQRQSDPARLLAPLRRRRDGRGFEPVGWDEALEEIARRLVRLRDESGP
                     LAALYYEGSGSHGALGGLAEAFWRQFGGCTRTHGDLCWPAGLEATRLTYGANLHNHPR
                     LTVESRLVVVWGHNPAETNVHQWRLILDAQSRGARLVVIDPRRTDSTDAADLHVQPKP
                     GTDAALALGIARIVVDEGLEDAAMLRAHAHGFAQYRARLADYPVQRVAEITGVGANTV
                     RELALEYGRVKPALLIAGFGLQRHHHAGQAMRAVALLPALTGNVGVPGGGWQYANLAS
                     HCLRPLPLPPEPPARRSVPVSRLAASLETLDAPPIRAAWIERANPGSQNPAAPRLRRA
                     LEALDLVVVVDQFMTPTAELAHFVLPAKTLFEEEDLVTAYWHPYLQWRARIVEPPGTV
                     RPETEIWRALSQRLGLDTSWFPGDREAFLRGLLPEPSEARLDELKKRPMDLSGLGDVA
                     FADRRFPTPSGKIEFASDQAAERWGADRIPGYVALGEGSESPLRSRFPLQLLSCKTRE
                     RIHSQFGNLAWVRGIERPHALDIHPDDAGPRGVQDGARVAVWNERGRIELRARLTPGI
                     RPGVVHVLEGRCHEGDPDVNALVAEGVTDMNHGATFYECLVEVAPV"
     gene            3416..4045
                     /locus_tag="PLT35_05215"
     CDS             3416..4045
                     /locus_tag="PLT35_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG28660.1"
                     /translation="MSGEHAGSAAGRGARSPGAGFTLDLHRCVGCGACVLACRIENGR
                     TGTAPWRRVVPVNLVRRLAGPTYFLSVACHHCDKPACLAGCPSRAYEKRADGVVLHHA
                     ERCLGCRYCEMACPFGAPQYDPDRGIVSKCTLCAPRLDDGRLPACVTACPTGALQLAP
                     PGRDTGGEALRDVPGFVDPAGCGPSLRFRAPAGARREQLFRALQEAMAR"
     gene            4042..4851
                     /locus_tag="PLT35_05220"
     CDS             4042..4851
                     /locus_tag="PLT35_05220"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HOG28661.1"
                     /translation="MKDGHGPLVAFTSLAIAGAGLVAADAAVGAPGESTGFAAAGAAL
                     MGAGIAVSLVHLGQKRRAGLAARGVGRSALSNEAAAAIVAALAAAAAALFGAAGRPNA
                     AVSAVAGLASAAFLLSIGLVYRVGGQRTWRGFTVATPLTGGLAFGAVTLHASSAAGGI
                     PAETLLVVVVDTLVFSRRWRDIVAMRVPAAVGTDAWWRRRAQALGARFFLLDALPVFL
                     LATGAARTAAICAAAGLVVDRVAFYALAVQHTTEFEAALVEERLGRMETNP"
     gene            5013..6614
                     /locus_tag="PLT35_05225"
     CDS             5013..6614
                     /locus_tag="PLT35_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG28662.1"
                     /translation="MMRTRTVVYCSAAAVIIAVAAGPIEPGRVAASSQQKVLRLATTT
                     STVDTGLLADILPDFQARCGCRVDVVAVGTGQAIELGKRGDADVLLVHSPEQEAAFMK
                     EGHGIRRDDVMYNDFVIVGPTGDPAGVAKASSARDAFRSIARAEAAFASRGDRSGTHT
                     KERAIWKSAGIDPAQEGPQRPSWYLSVGQGMGETLAFANEKQAYALADRATWTSMQGR
                     LPSLRRLFGGGSGDENPDRDLRNQYSVIVIDQARHPGVKAALGLQFADWLLSKPTQAR
                     IAAFGRDSAGRSLFYADSDEYKATDRVRVESGDRSRTFTLGEMAKLRSTTLSGVEYIG
                     VKKGPLGRHTWTGASLTDLLLALDPGIASKSRSTSTIEVVSSDGWTASFTWHELFGSV
                     SRGEGLYRAKGCNECHGLKAEGTRPEGKRPAPKLLGETYPVGDTTAMIRSGTAQHEGI
                     TAYTADRLSDADLAAILGWLARPNQAGTDDVFVVPPTRRIAMLAYERDGRPMTGRDGL
                     IQLVVGFDEFASRYSHWVSEIRVRY"
BASE COUNT         1039 a         2368 c         2434 g         1029 t
ORIGIN      
        1 cacgaggcct ggccggccaa ggacgcgaaa gccatggctg cgctgcttcc tgacatcgag
       61 cggcacgcgg ctgcggtcgc caacgccgtg ctgcccggca tcctccgcga caagcaggcc
      121 gcgtggctgg cgaaggtcac cgacttgaag gccgccgttg cctcttacaa ggcggccgtc
      181 ggctccggcg accacgaggc gctgttcaag gccgccgaga aggtccacgc ggagtacgag
      241 gggctcgtga agatcgtccg ccctgtcctc aaggagatgg aggagttcca cgccgccctc
      301 tacgtcctgt atcaccatca gttgagtccc ttccagatgg cgaaggtcag tgagtcggtc
      361 ggcatcctga agctgaagat ggatgcgctg aacacggccg tcctccccgc gcgcctgagc
      421 gccaaggccg acgccttcgg cctgcagcgc gcgcgcctca gcaaggcggt tgacgatctt
      481 gtcgcggccg tggctggacg agacgaggtg aaggtgcggg ccgccatcga gcagatgcac
      541 gctgaatacg agaacctcga gcgggttttc tagcagcgac caacggggca acggggtcag
      601 atcccaagaa gcgtgccatt ggcacgcttc ttgggatctg accccacatc cagtgtcgca
      661 gcggctgcac gctgtgtcgg accgcgcggt gcagctttct cgatcgccgg tgcttctgcc
      721 ggcggccccc cggtgcggac gccgctgcgc ccgggcagaa cgctcgacgc caagccattg
      781 acaaccggga cgttcagcgt cgtggggttg acggacggcg tcacggccgc actcgatgtt
      841 gcacgcggct gcacgtcccg gcggcaggca tcgaacttgc tgctacgacg agtgccgagg
      901 caggcaccgc cgtctccgcc tctccagacg gcggcgtgct tggttggcat cgaggtggcc
      961 atgtccgcag acgtcgaatg cgcttccgtc tccgagatgg cggtcaagac cctcgcgtgc
     1021 gccgtcatca agcaggccct ctgcgacgcg ctcgatccga cggtccccga gagaatccgc
     1081 cgcgacgccg agcagttcct ggcaggcgac gtgcgcttcc gccgctggtc ggccgtcgcc
     1141 ggcgttccac ccgacggcct gctctcgcgc cgactcaccg cctgaacccg ccgcccgcca
     1201 ttcggccgcc ggtggcggca acgcccatcc agcggccaag ggcacaaggc cccgcagccg
     1261 atgaagagtc ggcgtagcgg ctgcgagact gtccagacgc aagaggctgt gtccagcacg
     1321 cgaagaatcg gacacgccca gcgtgcgttg acacgaggcc gacccccact cgcgtttcca
     1381 gcgggtattc agctacactc ccggccgtgc cagccacgcg gaccattcgg accgtctgcc
     1441 cgcgcaactg ctactgcaca tgcgggatgc tcgtctcgct cgacgaacgg gaccgtatcg
     1501 tcgccatcga gggggatccc gagaacccgg caactggcgg ccgcgtctgc ctcaagggac
     1561 tcgcctacgc ccagcggcag tccgatccgg cgcgcctgct ggcgccgctg cgccgcaggc
     1621 gggacggacg cggcttcgaa ccggtgggct gggacgaggc gctcgaggag atcgcccgac
     1681 gactggttcg cctcagggac gaatcggggc cgcttgcggc gctctactat gaaggctcgg
     1741 gctcacacgg cgcgctcggg ggactggcgg aggcgttctg gcgccagttc ggcggctgca
     1801 cccgcacgca cggcgatctc tgctggccgg cgggcctcga agccacgcgc ctgacctacg
     1861 gcgccaacct gcacaaccat ccgcggctga ctgttgaaag ccgcctcgtc gtggtctggg
     1921 ggcacaaccc cgccgagacg aacgtccacc aatggaggct cattctcgac gcccagtcac
     1981 gcggggccag gctcgtcgta atcgatccga ggcgcacgga ctcaaccgac gccgcggacc
     2041 tgcacgtgca gccaaagccg ggcacggacg ctgcgctcgc gctgggcatc gcccgcatcg
     2101 tcgtggacga agggctggag gatgcggcga tgctccgggc gcacgcgcac ggtttcgcgc
     2161 agtacagggc caggctggcc gattatcccg ttcagcgcgt ggcggagatc acgggcgtcg
     2221 gggcgaacac cgtgcgcgaa ctggccctcg agtacggccg cgtcaagccg gcgctcctca
     2281 tcgcgggatt cggcctgcag cggcaccatc acgcgggcca ggccatgagg gccgtcgccc
     2341 tgctcccggc cctgacgggc aacgtgggcg tgccgggggg aggctggcag tacgccaacc
     2401 tcgccagcca ctgcctgcgt cccctgccgc tgccccccga accgcccgcg cgacgctcgg
     2461 ttccggtgtc gcggctggcc gccagcctcg agacgctcga tgctccgccg atccgcgccg
     2521 cgtggatcga acgcgcgaac cccggctcgc agaacccggc ggcgccgcgc cttcgccgcg
     2581 ccctcgaggc gctggacctt gtcgtcgtgg tcgaccagtt catgacgccg acagccgaac
     2641 tcgcccactt cgtgctgccg gcgaaaacgc tcttcgagga ggaggatctc gtcacggcgt
     2701 actggcatcc gtacctccag tggcgcgcca ggatcgtcga gcccccgggc accgtcaggc
     2761 cggagaccga gatctggagg gctctcagcc agcggctcgg cctggacacg agctggttcc
     2821 ccggcgatcg cgaagctttc ctgcgaggcc tcctgccgga gccgtcggaa gcgcgactgg
     2881 acgaactgaa gaagcggccg atggacttgt ccggcctcgg ggacgtggcc ttcgccgacc
     2941 gtcgatttcc gacgccgtcg ggcaagattg aattcgcctc ggaccaggcg gcggagcgct
     3001 ggggcgccga tcggattccg gggtacgtgg cgctcggcga gggttccgag tctccgctgc
     3061 gatcgcgctt cccgctgcag ctgctctcgt gcaagacgcg cgagcgcatc cactctcagt
     3121 tcggcaacct cgcgtgggtc cgcgggatcg agcggccgca cgcgctcgac attcatccgg
     3181 acgatgccgg gccgagaggc gtgcaggatg gcgcacgcgt cgcggtctgg aacgaacgcg
     3241 ggcgaatcga gcttcgcgcg cgcctgacac cgggaatcag gcccggcgtc gtccacgtgc
     3301 tcgagggccg ctgccacgag ggcgaccccg acgtgaacgc gctcgtggcg gagggcgtca
     3361 cggacatgaa ccacggcgca acgttctacg agtgcctggt cgaggtggct cctgtatgag
     3421 cggcgaacac gcgggcagcg cggcagggcg cggcgcgcgc tcgcctggcg ccggtttcac
     3481 gctcgatctg caccggtgcg tgggctgcgg agcgtgcgtg ctggcgtgcc gcatcgaaaa
     3541 cggccgcacc gggacggccc cgtggcgccg ggtggtgccg gtcaacctcg tccgccggct
     3601 ggccgggccg acctacttcc tgtccgtcgc gtgccaccac tgcgacaagc ccgcctgcct
     3661 ggcgggctgc ccgtcgcgcg cgtacgagaa gcgggccgac ggagtcgtgc tccaccacgc
     3721 ggagcgctgc ctcggctgcc ggtattgcga gatggcttgc ccgttcggtg cgccgcagta
     3781 cgacccggac cggggcatcg tctccaagtg cacgctgtgc gcgccgcggc tggatgacgg
     3841 ccggcttccg gcctgcgtga cggcctgtcc cacaggcgcg ctgcagctgg cgccgcctgg
     3901 cagagacacc ggcggggaag cgctgcgcga cgtccccggg ttcgtcgatc ccgccggctg
     3961 cggaccctcg ctgcgcttca gagctcctgc cggagcgcgg cgcgagcagc tgttccgggc
     4021 gcttcaggag gcgatggcgc gatgaaggac gggcatggtc cgctcgtggc gttcacgagc
     4081 ctcgccatcg ccggcgcagg actggtcgcc gccgacgccg ccgtcggcgc ccccggagag
     4141 agcaccgggt ttgcggcagc tggcgcggcg ctgatgggtg ccggcatcgc cgtgtcgctg
     4201 gtccacctcg ggcagaagcg ccgcgcgggc ctggccgcac gcggggttgg ccgcagcgcg
     4261 ctcagcaacg aggcggcggc ggcgatcgtc gcggcgctcg ctgccgccgc cgcggccctg
     4321 ttcggagcgg ccggccgtcc gaacgccgcc gtctccgccg tcgccggtct ggccagcgcg
     4381 gcattcctcc tgtcgatcgg cctcgtctac cgcgtcggcg gacagcgcac gtggcgggga
     4441 ttcaccgtcg cgacgccgct caccggcggc ttggcctttg gcgccgtcac gcttcatgct
     4501 tcctcagcgg cgggcggaat cccggcggag acgctgctgg tcgtggtcgt cgacaccctg
     4561 gtcttttcgc gacggtggcg cgatatcgtg gcgatgcgcg tgcctgcggc cgtgggaacc
     4621 gatgcgtggt ggcgccgccg ggcccaggcg ctcggcgcga gattcttcct gctggatgcc
     4681 ctgccggtct tcctcctggc gacaggagca gcgcgcaccg ccgcgatctg cgccgcagcc
     4741 ggcttggtcg tcgaccgagt cgcgttctac gcgctggccg tccagcacac gaccgagttc
     4801 gaggccgcct tggtcgagga acgcctgggc cggatggaga cgaacccgta gcgcgcgccc
     4861 agaccggcgc agcggtgggc gggcccatgg gccaggtgca gggccacgcg agaggccgta
     4921 cggcgggccg gccgcggagc cccgagacgc acacagccgg gcagttgggg tacgcccgtc
     4981 ttccgagtac gattctagcg gaggccgatc acatgatgcg cacccgaacc gtcgtctact
     5041 gctctgccgc cgccgtgatc atcgctgtcg ccgccggtcc aatcgaaccg ggccgggtcg
     5101 ccgcctcgtc gcagcagaag gttctgcggc tcgccacgac aacgagcacg gtggacaccg
     5161 gactgctcgc ggacatcctg ccggacttcc aggcgcggtg cggatgccgg gtggacgtgg
     5221 tggccgtcgg aacgggccag gcgatcgagc ttggcaagcg cggcgatgcg gatgtgctgc
     5281 tcgtgcattc gcccgagcag gaagctgcgt tcatgaagga aggccacggc atcaggcgcg
     5341 acgacgtgat gtacaacgac ttcgtcatcg tgggaccgac cggggatccc gccggtgtgg
     5401 ccaaggcgtc gagcgccaga gacgccttca gatccattgc cagggcggaa gcggcattcg
     5461 caagccgcgg cgacaggtcc gggacgcaca cgaaggaacg agcaatctgg aagtcggcgg
     5521 gcatagaccc cgcgcaggag ggtccgcagc ggccgtcctg gtacctctcg gtaggacaag
     5581 gcatgggcga gaccctcgcc ttcgcgaacg agaagcaggc gtacgccctc gccgaccgcg
     5641 ccacctggac ctcgatgcaa gggaggctgc ccagcctgcg gcgcctcttc ggcggcggat
     5701 ccggcgacga gaatccggat cgcgatctgc gcaatcagta cagtgtgatc gtcatcgacc
     5761 aggcgcggca cccgggcgtg aaggcggcgc tgggactgca gttcgccgac tggctgctgt
     5821 cgaagcccac gcaggctcgg attgccgcgt tcgggcgcga cagcgccggg cgttcgctgt
     5881 tctacgccga ctcggacgag tacaaggcga cagaccgcgt ccgcgtcgag tccggcgatc
     5941 gatcgcgcac gttcacgctc ggggagatgg cgaagctcag gagcaccacg ttgtccggcg
     6001 tggaatacat cggcgtgaag aaggggccgc tgggacggca cacctggacc ggcgcgtcgt
     6061 tgacggatct gctcctcgcc ctggaccccg ggatcgcatc gaagtccagg tcgacgtcaa
     6121 cgatcgaagt cgtcagcagc gacggctgga cggcgagctt cacgtggcac gaactgttcg
     6181 ggtcggtcag ccgcggtgaa gggttgtacc gggccaaggg ctgcaacgag tgccacggcc
     6241 tgaaggcgga gggcacgcga cccgagggga agcggccggc cccgaagctg ctcggcgaaa
     6301 cgtaccccgt cggggacacg acggcgatga tccgctccgg caccgcgcag cacgagggca
     6361 ttacggccta cacggccgat cgcctctccg acgcggatct ggcggcgatt cttggctggc
     6421 tggcgcggcc gaatcaggcg ggcaccgacg atgtgttcgt cgtaccgcca acgcggcgaa
     6481 tcgcgatgct ggcgtacgag cgcgacggtc ggccgatgac gggccgcgac ggccttatcc
     6541 aactggttgt ggggtttgac gagttcgcat cgcgctactc gcattgggtg tcggagatcc
     6601 gcgtccgcta ctgaaggggt cgggacgcat ttggcaacgc cgcagttgac gattacagaa
     6661 gcgtcagaag gagcaccaat gacgtttttg tatctgtcgt gggttcgagt gcgtaatgag
     6721 acccgacccc ttgagctatg atcgacgaca gcctgcgcgc gctcggcgat cccgccctcc
     6781 tgagtatcgt gtttctgacg ttccgcgtca cgggccttgc gctcgtgatc gcgaccgcca
     6841 tcggcgtgcc cgtcggggcg gccgtcggct
//
LOCUS       DAOSIF010000110         6838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10092_length_6838_cov_4.152145, whole genome shotgun sequence.
ACCESSION   DAOSIF010000110 DAOSIF010000000
VERSION     DAOSIF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10092_length_6838_cov_4.152145"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLT35_05230"
     CDS             <1..839
                     /locus_tag="PLT35_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOG28663.1"
                     /translation="ILEQEKLVRGLPDLGMNRRALGLFQDLLGRSRGIVLVTGPTGAG
                     KTTTLYSALNALGGRARNIMTVEDPVEFEFPAIRQTQVRPRAGLTFAAAIRSMLRQDP
                     DVIMVGEIRDPETAELAVRAALSGVLVFSTLHTQDAAGAVPRLMDMGVEPYLLASTMA
                     GVVAQRLVRLVCQRCKAPASYPAASLARAGLSHADGVAFVKGAGCEDCGGSGYRGRTG
                     VFEILMIDAAMRELISARADSRRIRDTAARAGFKPIGDDALSKAVLGQTTLEEVIRVS
                     SE"
     gene            851..2077
                     /locus_tag="PLT35_05235"
     CDS             851..2077
                     /locus_tag="PLT35_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG28664.1"
                     /translation="MPEFSYVAIGPDGRRVSGRAAAASEEALADALRTRGQYLVEAGP
                     AGAAGAGARLASIRVLDRVTRRDVIVFTGQLAAVVAAGVNLVDGLRDIEAQAVKAPLK
                     AVIAGVRRGVERGQSLSAAMALHPAAFNELYVSIVRAGEASGRVDRALDDLVQQLEWQ
                     EALAGKVREASTYPLIVVGLLAVLLTVLVGFTIPRFARVYQNVNANLALPAPTRAVQA
                     VAAAAASNALVIAFALVAAYVLYRLRVQAPGGRAWRDRWLLRLPLAGDVARKVALSRF
                     AHAFGSLHQSGVEVAPSLSLVERVIGNAYIAGRFRAAVSRVLAGEPLSRALAAVGEFS
                     PLVIQMVALGEKTGEMEKSLRQVRQYYDREVDRSVNRAITLFGPAALGALALVFVLIA
                     VAFYLPMFNLARALTR"
     gene            2074..2838
                     /locus_tag="PLT35_05240"
     CDS             2074..2838
                     /locus_tag="PLT35_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG28665.1"
                     /translation="MIPKRARDCGFTLIEAAVILAVLAVLAAAITPMILQQIVEAKTE
                     ATRREARLLHEAMLGRPDVPDSFGFFGDMGRLPASFEELVRPGPGTALFGTATFRNVG
                     MGWKGPYINAGDAAGDYLTDAFGRPYRGASAGQVRSAGPDGVFDTADDIVYPPARPNP
                     TGRLMVTVKRMSSEDASYTLDPAGYAVRLYYSNGGVQASLDDAVPPFVFENVPQGVHA
                     IAVLRMKSDPPQIVAQDTILVFGGGSTRLVELIFRP"
     gene            3046..3726
                     /locus_tag="PLT35_05245"
     CDS             3046..3726
                     /locus_tag="PLT35_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28666.1"
                     /translation="MALIETTDLWKTYVVGTEEIHALRGVDVTIERGEYVAIMGPSGS
                     GKSTLMNLIGCLDTPSKGSYLLNGKQVGQMDDDELARIRNEEIGFVFQTFNLLPRATA
                     LHNVELPLVYAGVPAKTRLDRAKTALEKVELADRMTHRPNQLSGGQRQRVAIARALVN
                     NPSILLADEPTGNLDSKTGVEIMTLFDRLHAANNTIILVTHEADIARHAKRIIHIRDG
                     KVEIDERR"
     gene            complement(3756..4574)
                     /locus_tag="PLT35_05250"
     CDS             complement(3756..4574)
                     /locus_tag="PLT35_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOG28667.1"
                     /translation="MAMVEVRGLEKHFVRRAAPWGRPTRVTAVDGFDLSIEEGEIVGL
                     IGESGSGKTTAGRCLLRLVEPSAGSFAFRGEDVFAMSPARLRQARRQMQMVFQDPESS
                     LDPRMRAGAIVEEGLVIHGLGTPAERRGRVREMLELVGLDANRADRYPHEFSGGERQR
                     LGLARALALAPSFLVADEPVSSLDLSIQAQIVNLFMDLQQQLGLTLLFITHDLRLVRH
                     LCSRVVVMHRGRTVGAGPAADVDRLWGQISTGEPAGSDLEFRSPPGRRAAEFEV"
     gene            complement(4574..5596)
                     /locus_tag="PLT35_05255"
     CDS             complement(4574..5596)
                     /locus_tag="PLT35_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28668.1"
                     /translation="MEANARDNTLLDVRALAVEFAAPRRGLRASIGRAPRAGVRVVDG
                     VSFHIARGETLGLVGESGSGKSMTALALAGLVPKPGRIAGGTVRFEGRELTSLREAEW
                     RRIRGARIGFVFQEPSSALNPVFTIGSQIAEALEVHGRASGSAARARAVELLDAVRIP
                     SPAARAGDYPHQWSGGMKQRALLAAAIACDPALLIADEPTTALDVRIQAEILDLLAEL
                     RDRLGLSLLVVTHDFGVVERLADRVAVMYAGRAVESGPAREVLAAPSHPYTRGLLASR
                     PGATPGRRLAAIPGSVPDPAALPRGCAFAPRCPERIEACDERRPGATAIAPGREVRCL
                     LREGTA"
     gene            5797..>6838
                     /locus_tag="PLT35_05260"
     CDS             5797..>6838
                     /locus_tag="PLT35_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28669.1"
                     /translation="MSNRRWGLTPLIIILCILSAVAFVPVVAQQQAQKVDKKVLKAQE
                     ADLDAIIKVSNAVLMGQEPAPTDYALTFRSDFLKAQDGKTFVPFTVTVDPSQVPPGQN
                     VTFFVRVVAKTPPAASAPDAKKDDRNKKGAGPEFVHQDASFITAAAPAKPGDPFKISR
                     AFAVLPGEYDVYLALKERLPIDAKNREKTPTKMGILKQTVGVPNLWTDSLLTSSIIVA
                     DKVETLTEPLTREQQIEQPYTFGLTQITPALVNRFTKKGEISLLFLIYNTAMDADKKP
                     DVSVDYNFYQKDAAAENGEKFFNRTNPQVFNAKTLPPQFDPALGHQLVAGQSLPLGSF
                     PEGEYRLEVTVTD"
BASE COUNT         1050 a         2430 c         2416 g          942 t
ORIGIN      
        1 gcatcctcga gcaggagaag ctcgttcgcg gcctgcccga cctcgggatg aaccgccgcg
       61 ccctcgggct cttccaggac ctgctcggcc gctcgcgcgg catcgtcctg gtgacgggcc
      121 cgaccggcgc gggcaagacg accacgctct actcggcgct gaacgcgctg ggcggccgcg
      181 cgcgcaacat catgaccgtc gaggacccgg tcgagttcga gtttcccgcc atccgccaga
      241 cccaggtgcg gccgcgcgcg ggcctcacct tcgcggcggc catccgatcg atgctccgcc
      301 aggaccccga cgtcatcatg gtcggcgaga tccgcgaccc ggagaccgcc gagctggccg
      361 tccgcgcggc gctctccggc gtcctcgtgt tctccacgct gcacacgcag gacgcggcgg
      421 gcgcggtgcc gaggctgatg gacatgggcg tcgagccgta cctgctcgcc tccaccatgg
      481 cgggcgtcgt ggcgcagcgg ctcgtgcgcc tcgtctgcca gcggtgcaag gcgccggcgt
      541 cgtacccggc tgcctcgctc gcccgcgccg ggctgtcgca cgccgacggc gtggcgttcg
      601 tcaagggcgc cggctgcgag gactgcggcg gctcgggcta ccgcggcagg acgggggtct
      661 tcgagatcct catgatcgac gcggcgatgc gcgagctcat ctccgcccgg gccgactcgc
      721 gccgcatccg cgacacggcc gcgcgcgccg ggttcaagcc gatcggcgac gacgccctgt
      781 cgaaggccgt cctcgggcag accacgctcg aggaggtgat ccgcgtgtcc tcggagtgag
      841 ccggcgcgcc atgcctgagt tctcgtacgt cgcgatcggg ccggacggca ggcgcgtgtc
      901 gggccgcgcc gcggccgcca gcgaggaggc gctcgccgac gccctgcgca cgcggggcca
      961 gtacctcgtc gaggccgggc cggccggcgc cgccggggcc ggcgcgcgcc tggcctcgat
     1021 ccgcgtcctc gaccgcgtca cccgccgcga cgtgatcgtc ttcacgggcc agctcgccgc
     1081 cgtcgtcgcc gcgggcgtga acctggtcga cggtctgcgc gacatcgagg cgcaggccgt
     1141 caaggcgccg ctcaaggccg tgatcgccgg cgtgcggcgc ggcgtcgagc gcggccagag
     1201 cctgtcggcg gcgatggcgc ttcatcccgc ggcgttcaac gagctttacg tcagcatcgt
     1261 ccgcgcgggg gaggccagcg ggcgcgtgga tcgcgccctc gacgacctgg tgcagcagct
     1321 ggagtggcag gaggcgctgg ccggcaaggt gcgcgaggcc tctacctacc ccctcatcgt
     1381 cgtcggcctg ctggcggtgc tgctgaccgt gctcgtcggc ttcacgatcc cgcgctttgc
     1441 gcgcgtgtac cagaacgtga acgcgaacct cgcgctgcca gcgccgacgc gcgccgtcca
     1501 ggccgtcgcc gccgccgccg cgtcgaacgc cctcgtcatc gcgttcgcgc tcgtcgccgc
     1561 gtacgtcctc taccggctgc gcgtgcaggc gccgggaggc cgggcgtggc gcgaccggtg
     1621 gctgctgcgc ctgcccctgg cgggcgacgt cgcgcgcaag gtggcgctct cgcgcttcgc
     1681 ccacgcgttc ggcagcctgc accagtcggg cgtcgaggtg gcgccgtcgc tgtcgctcgt
     1741 cgagcgcgtc atcggcaacg cctacatcgc cgggcgcttc cgcgccgccg tgtcgcgcgt
     1801 gctggccggc gagccgctct cgcgagccct ggcggccgtc ggcgagttct cgcccctcgt
     1861 catccagatg gtggcgctcg gggagaagac cggggagatg gagaagtcgc tgcggcaggt
     1921 gcggcagtac tacgaccggg aggtggaccg gtccgtgaac cgcgccatca cgctgttcgg
     1981 gcctgcggcg ctcggggccc tcgcgctcgt cttcgtcctg attgccgtcg ccttctacct
     2041 gccgatgttc aacctcgcga gggcgctgac ccgatgatcc cgaagcgcgc gcgcgattgc
     2101 ggattcacgc tgatcgaggc ggccgtcatc ctggcggtcc tggccgtcct cgccgccgcc
     2161 atcacgccga tgatcctgca gcaaatcgtc gaggcgaaga cggaggccac ccggcgggag
     2221 gccaggctgc tgcacgaggc catgctcggg cggcctgacg tcccggacag cttcggcttc
     2281 ttcggcgaca tggggcggct gccggcgtcg ttcgaggagc tggtcaggcc gggccccggc
     2341 acggcgctgt tcggcacggc gacgttccgg aacgtcggaa tgggctggaa ggggccgtac
     2401 atcaacgccg gggacgccgc cggcgattac ctcaccgacg ccttcgggcg cccctaccgg
     2461 ggtgcgtctg ccgggcaggt gcgcagcgcc gggccggacg gcgtgttcga caccgccgac
     2521 gacatcgtgt atccgcctgc gcggcccaac ccgaccgggc ggctgatggt gaccgtcaag
     2581 cgcatgtcgt cggaggacgc gagctacacc ctcgacccgg ccggctacgc cgtccgcctc
     2641 tactattcga acggcggcgt gcaggccagt ctggacgacg ccgtcccgcc gttcgtgttc
     2701 gagaacgtgc cgcagggcgt ccacgccatt gccgtgctgc gcatgaagag cgacccgccg
     2761 cagatcgtcg cgcaggacac gattctggtg ttcggaggag gctccacccg cctcgtcgag
     2821 ttgattttcc gcccgtaagg agaatctggg gtcagatcca gcattgcggg gtcagatcaa
     2881 ccccgcaatc ctggatctga ccccgcaatg ctggatctga ccccaggact ccgcgtggta
     2941 ctctctcgga ggggcaggaa ccgcgcgggt gcgtttcccg tctgtgatcg atcatagagg
     3001 ccgacggccc gggacctctc ccggccgcgg ggtggacgac ggcttatggc gctgattgaa
     3061 acgacagacc tgtggaagac ctacgtggtg gggaccgagg aaatccacgc gctgcgcggc
     3121 gtcgacgtga ccatcgagcg cggcgagtac gtggcgatca tgggtccgtc gggctcgggg
     3181 aagtccaccc tcatgaacct gatcggctgt ctcgacacgc ccagcaaggg cagctacctg
     3241 ctcaacggca agcaggtggg ccagatggac gacgacgagc tggcgcgcat caggaacgag
     3301 gagatcggct tcgtgttcca gacgttcaac ctgctgccgc gggccacggc gctccacaac
     3361 gtggaactgc ccctcgtgta cgccggggtg cccgcgaaga cccgcctcga tcgggcgaag
     3421 accgccctgg agaaagtcga gctggcggac cggatgacgc accggccgaa ccagctctcg
     3481 ggaggccagc gccagcgcgt cgccatcgcg cgggcgctcg tgaacaaccc ttccatcctg
     3541 ctggccgacg agccgaccgg caacctcgac tcgaagacgg gcgtcgagat catgacgctg
     3601 ttcgaccggc tgcacgcggc aaacaacacc atcatcctcg tcacgcacga ggcggacatc
     3661 gcccgccacg cgaagcggat catccacatc cgcgacggca aggtggaaat cgacgagcgc
     3721 cgttagcggg gaatcgacga gcgccgttag gggggtcaga cctcgaactc cgcagcccgt
     3781 cgacctggag ggctgcggaa ttcgagatct gaccccgcgg gttcaccagt cgagatctgt
     3841 ccccacaggc ggtcgacgtc ggcggcgggg ccggcgccga cggtgcggcc gcggtgcatc
     3901 acgacgacgc ggctgcagag gtggcgcacc aggcgcaggt cgtgcgtgat gaacagcagc
     3961 gtcagcccga gctgctgctg caggtccatg aacaagttga cgatctgcgc ctggatggac
     4021 aggtccagcg acgacaccgg ctcgtcggcc acaaggaagc tcggcgcaag ggccagggcg
     4081 cgcgcgaggc cgaggcgctg gcgctcgccg ccgctgaact cgtgcgggta gcggtcggcg
     4141 cggttcgcat ccaggccgac cagctcgagc atctcgcgca cccgcccacg ccgttcggcc
     4201 ggggtcccga ggccgtggat cacgaggcct tcctcgacga tcgcgcccgc acgcatccgc
     4261 gggtcgagcg acgattcggg gtcctgaaag accatttgca tctgacgccg ggcctggcgc
     4321 agccgcgccg gcgacatcgc gaagacgtcc tcgccgcgga acgcgaacga accggccgac
     4381 ggctcgacga gccgcagcag gcaccgtccg gccgtcgttt tgccgctgcc cgattcgccg
     4441 atcaggccga cgatctcgcc ctcctcgata gacaagtcga agccgtccac cgccgtgacg
     4501 cgcgtcggcc gcccccaggg cgccgcccgc cgcacgaagt gcttctcgag gccgcgcacc
     4561 tccaccatcg ccatcacgcg gtcccttccc tcaggaggca gcggacctcg cggccggggg
     4621 cgattgccgt tgcgcccggc cgccgctcgt cgcacgcctc gatccgctcg gggcagcgcg
     4681 gggcgaaggc gcagcctctc ggcagggcgg ccggatcggg cacgctgccc gggatcgccg
     4741 ccaggcgacg gcccggcgtg gccccgggcc gagacgccag cagcccccgc gtgtagggat
     4801 gggacggcgc ggcgaggacc tcccgcgcgg gcccgctctc gacggcgcgg ccggcgtaca
     4861 tgacggccac gcgatccgcc aggcgctcca cgacgccgaa gtcgtgagtc acgacgagga
     4921 gcgacaaccc gaggcgatcg cgcaactccg cgagcagatc gaggatctcc gcctggatgc
     4981 gcacgtcgag cgccgtggtc ggctcgtcgg cgatgaggag cgccgggtcg cacgcgatgg
     5041 cggcggcaag cagggcgcgc tgcttcatgc cgccggacca ctgatggggg tagtcgcccg
     5101 cgcgcgccgc cggactgggg atgcgaacgg cgtccagcaa ctcgacggcc cgggcgcggg
     5161 ccgcggagcc cgacgcgcgg ccgtgcacct ccagcgcctc tgcgatctgg ctcccgatgg
     5221 tgaacaccgg gttgagggcc gacgagggct cctggaagac gaacccgatg cgggcgcccc
     5281 ggatccgccg ccactcggct tcccggagcg atgtcagctc gcgcccctcg aaacgcaccg
     5341 tgccgccggc gatccggccg ggcttcggaa ccaggcccgc gagggccagg gccgtcatcg
     5401 acttgcccga ccccgactcg ccgacgaggc caagggtctc gccgcgggcg atgtggaaac
     5461 tcacgccgtc cacgacccgg acgccggcgc gaggggcacg gccgatggac gcgcgcaggc
     5521 cccggcgggg cgcggcgaac tccaccgcca gggcccggac gtcgaggagc gtgttgtcgc
     5581 gagcgttcgc ttccattccc acgcgccccg cggccgaggc cgggccgcgg cccggccggc
     5641 cgcccggccg ccgggcaggc gcaaacaccg ggccggcgga cgcgacggaa tcttagcaca
     5701 gccaatcggc gcaaaccttt gacacggcgt ggccttgggc ctatgatcga cgggtgcccg
     5761 accatggtgg aaggcaagac ggggagtagc gtaatcatga gcaatcgccg gtggggactg
     5821 acgccgctga tcatcatcct gtgcatcctg tccgcggtgg cgttcgtgcc cgtcgtcgcc
     5881 cagcagcagg cccagaaggt cgacaagaaa gtcctgaagg cccaggaagc cgacctcgat
     5941 gccatcatca aggtctcgaa cgccgtgctg atggggcagg agccggctcc gacggactac
     6001 gcactgacgt tccgcagcga ctttctcaag gcccaggacg ggaagacgtt cgtgccgttc
     6061 acggtgacgg tcgatccgtc gcaggttccg ccgggccaga acgtgacgtt cttcgtgcgc
     6121 gtcgtggcca agacgccgcc ggccgcctct gcgcccgacg ccaagaagga cgacaggaac
     6181 aagaaaggcg cggggccgga gttcgtgcac caggacgcgt cgttcatcac ggcggccgcg
     6241 ccggccaaac cgggcgatcc gttcaagatc agccgcgcat tcgcagtgct gcccggcgag
     6301 tacgacgtgt atcttgcgct gaaggagcgc ctgcccatcg acgccaagaa ccgcgagaag
     6361 acgccgacca agatgggcat cctcaagcag accgtgggcg tgccgaacct ctggaccgac
     6421 agcctcctga cgagctcgat catcgtggcg gacaaggtcg agacgctgac cgagccgctg
     6481 acccgcgaac agcagatcga gcagccgtac acgttcggct tgacgcagat cacgccggcg
     6541 ctggtgaaca ggttcacgaa gaagggcgag atctcgctgc tgttcctgat ctacaacacc
     6601 gcgatggacg ccgacaagaa gccggacgtg agcgtggact acaacttcta ccagaaggac
     6661 gcggctgccg agaacggcga gaagttcttc aacaggacga acccgcaggt gttcaacgcg
     6721 aagacgctgc cgccgcagtt cgatcccgcg ctggggcatc agctggtggc gggccagtcg
     6781 ctgccgctcg ggagcttccc ggagggcgag taccggctcg aggtcacggt gaccgaca
//
LOCUS       DAOSIF010000111         6829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10110_length_6829_cov_3.558164, whole genome shotgun sequence.
ACCESSION   DAOSIF010000111 DAOSIF010000000
VERSION     DAOSIF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10110_length_6829_cov_3.558164"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLT35_05265"
     CDS             complement(<1..264)
                     /locus_tag="PLT35_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HOG28670.1"
                     /translation="MLIYGINPVLEALKAGRVREVRIDGARRARAGDVCAAAAARGVP
                     VRVVPAPDLVDPASAPLLAEPFASAYAVADAGPAAVDAAAAAAG"
     gene            complement(284..1783)
                     /gene="gltX"
                     /locus_tag="PLT35_05270"
     CDS             complement(284..1783)
                     /gene="gltX"
                     /locus_tag="PLT35_05270"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG28671.1"
                     /translation="MRVRFAPSPTGLLHVGNARTALFNWLLARGGGGTFILRIEDTDA
                     ERSTPESERGILDDLRWLGLQWDEGPDVGGAYGPYRQSERLDLYRSYAGELLDAGQAY
                     YCFCSPDALERERQAALEAGRPPQYSGACRALPPAEAARRVAAGEAAAVRFRVPARRD
                     VAFEDVVRGTVRFDAGVIGDPVLLRSDGRPAYNFAVVVDDALMEVTHVVRGEDHVSNT
                     PRQVLLYEALHFEAPVFAHLALVLGPDHTPLSKRHGATSVAEFRNRGILPEALLNYLA
                     LLGWSPGDSEEILPAEELARRFSLEHVGRSSAVFDVEKLAWVNRHYLRDAAPARLAAL
                     AMPHLVEAGFVLAETATARDFAAAVMPLAAASVDRIEQIPARIRFLFEFDPGRARALE
                     EVGRVLAEPGASAVIAALAADLAGAPRLVDRERFRAAAARIRQATGCKGRALFHPIRV
                     ALTGEAGGPELDLAVPAIERGAELPPGSGVAPVLGCRERAARFAGELQK"
     gene            complement(1800..2960)
                     /gene="ispD"
                     /locus_tag="PLT35_05275"
     CDS             complement(1800..2960)
                     /gene="ispD"
                     /locus_tag="PLT35_05275"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOG28672.1"
                     /translation="MRVAAIIAAGGRGERLGAGVPKQLLPVGGVSILARSVEIFAGHD
                     LVDEIVVVLPAELAADPPAGLHAGAKPLRVVAGGARRQDSVRRGFEAVAGRADLVVIH
                     DAARPFASLSLVTRVIEAAEESGAALAAVAASDTVKLAREDAAGGAVVERTLPRARIH
                     LAQTPQAFRTEVLAAAIEAGRGIEATDEAALAEAAGFPVRLVPGEASNMKITTMTDLT
                     TADAVAGGPGARVGAGYDLHRLVEGRPLVLGGVTIPFDRGLAGHSDADVAAHAVTDAV
                     LGAIAGGDIGRHFPDADPRWKGASSLAMLAHAAGVARDAGFEVVNVDVVIVAERPKIA
                     PYADAIRAGLAGALGIAVARVSVKGKTNEGLDAVGRGEAMAAHAVALVSPRG"
     gene            complement(2962..3615)
                     /locus_tag="PLT35_05280"
     CDS             complement(2962..3615)
                     /locus_tag="PLT35_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOG28673.1"
                     /translation="MTGDETAGVAIEPGLERIVRLGVLWCAVAPAPGADRALDAPLAE
                     AVGAARGGDVADTSAARALYRAIGVDPTKTRPSSEALLRRVRRGGPFPRINALADIAN
                     WCSLQTQLPYGLYDAAAIRPPLVLRRGGPGDAYAGIRKDAVRLDGRLALFDALGPFGN
                     PTSDSARTMTRAATRDVLVAVFAPAAVPRERMARVLDMTSARLAEYAGGAETGRWVS"
     gene            complement(3612..4367)
                     /locus_tag="PLT35_05285"
     CDS             complement(3612..4367)
                     /locus_tag="PLT35_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG28674.1"
                     /translation="MTLPSPAWWRTLLVTLPAVVVLTVAAGVAALATTLFDRSGRAAH
                     RLAQWWGRGVLAAAAVRVRVRGLDALVPGATYVFVANHQSLYDIPVLFGSIPYQIRII
                     AKQSLGPIPFVGWHLRRAGHILVDRAHPDREGILRRWRGLVGEGLSLIIFPEGTRSAD
                     GTVRRFKSGSFTLALQAGLPVVPLSIAGTRDVMRKGEFTVRPGVVSLVVHPPIETRGR
                     AAEPGIEDVRAFAAEVRAIVAGGVEALGRAGAA"
     gene            complement(4364..5437)
                     /locus_tag="PLT35_05290"
     CDS             complement(4364..5437)
                     /locus_tag="PLT35_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOG28675.1"
                     /translation="MAWFLLARAIFLGAVTYTAAVLLPLEGAGLAANASLGAAIALAA
                     IALEWRMRDVSVPRILGAAIGAALGLFIAKLVTGALMWSDRGHGAFVQRVILLLFPYL
                     GLIIGARRGEWLQPARLTALFRGSAAARRQTVLDTSVVIDGRVADVCEVGFLDGTLVV
                     PQFVLRELQQIADSTDTMRRNRGRRGLDILHRMRKMAGVDVVVSDTDFPDIREVDLKL
                     IELARSLNARIATNDLNLNKVARLRGVGVLNINDLANAVKPVVLPGEVMTVLILKEGK
                     EYNQGVAYLDDGTMVVVDNARRLLNRTIDITVTSVLQTTAGKMIFGRHIGAGTGPQAP
                     PEREDARGRTRAGPPPPPAAQDP"
     gene            complement(5482..>6829)
                     /gene="radA"
                     /locus_tag="PLT35_05295"
     CDS             complement(5482..>6829)
                     /gene="radA"
                     /locus_tag="PLT35_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOG28676.1"
                     /translation="ACQECGAQSPKWLGRCPDCGAWNSLVEERAPASAPAGRYPAPAG
                     GGARLYADIEASSAARIATGIGELDRVLGGGIVPGSLVLLGGEPGIGKSTLLLQTAAH
                     LARTAGPVLYSSGEESEHQVKSRGERLGVGDAPLYLLAETCLERVLEEMTRLSPALVI
                     VDSIQTVFSLRMQSAPGSIGQVREAATQLLFAAKAQNVPTFLVGHVTKEGSLAGPKAL
                     EHIVDTVLYFEGERQHAHRIVRAAKNRFGAVSELGVFEMTAAGLVPVPNPSAMFLAER
                     PANAPGSAVLCCIEGTRPLLVEIQALVSSSSYGYAKRTAVGLDQNRLSLLLAVLEKRA
                     GLALGGDDVYVNVAGGLSVDEPALDLAVVAAVASSFRNRPIAAGTAVFGEVGLAGEVR
                     ATAQAARRVREAAQMGFSRCVMPAASIDRKDPGFADAGCALAGVRTVGEALEHLLA"
BASE COUNT          919 a         2588 c         2507 g          815 t
ORIGIN      
        1 cccggctgcg gcggccgctg cgtcaacggc ggcggggccg gcatcggcaa cggcataggc
       61 gctggcaaag ggctcggcca gcagcggcgc actggccggg tcgaccaggt cgggcgccgg
      121 cacgacccga accggcacgc cccgcgcagc ggccgcggcg cagacgtccc cggcgcgcgc
      181 gcgccgcgcg ccgtcgatgc gcacctcgcg gaccctcccg gccttcagcg cctcgagcac
      241 gggattgatg ccgtagatca acatctgacg ccaggcatcc tgcctacttc tgcaactccc
      301 ccgcaaagcg cgccgcgcgc tcgcggcagc cgagcaccgg cgccacgccg cttcccggcg
      361 gcagctccgc gccgcgctcg atggccggca cggccaggtc gagctcaggc ccgccggctt
      421 cgcccgtgag cgccacgcgt atcgggtgga acagcgcccg gcccttgcac ccggtcgcct
      481 gcctgatccg ggcggctgcc gcgcggaagc gctcgcgatc gacgaggcgc ggcgcgccag
      541 ccaggtcggc ggcgagcgcg gcgatgaccg ccgatgcgcc cggttccgcc agcacgcggc
      601 cgacctcctc cagcgcgcgc gcccgtccgg gatcgaactc gaagagaaac cggatgcggg
      661 ccgggatctg ctcgatgcga tcgaccgacg cggccgccag cggcatgacg gccgcggcga
      721 agtcgcgggc cgtcgcggtc tccgcgagca cgaagccggc ctcgaccagg tgcggcatgg
      781 ccagggccgc cagccgggcg ggcgccgcgt cgcgaaggta gtggcggttc acccaggcga
      841 gcttctcgac gtcgaacacc gccgagctgc ggccgacgtg ctcgagggag aaccgccgcg
      901 cgagctcttc cgccggcagg atctcctcgc tatcgccggg cgaccagccg agcagcgcga
      961 ggtagttcag cagcgcctcg ggcaggatgc cccggttccg gaactcggcc acggaggtgg
     1021 cgccgtggcg cttcgagagc ggcgtgtgat cggggccgag cacgagggcg aggtgggcga
     1081 acaccggcgc ctcgaagtgc agcgcctcgt acagcaggac ctgccgcggc gtgttcgaga
     1141 cgtggtcctc gccgcgcacg acgtgggtga cttccatcag cgcgtcgtcc acgaccacgg
     1201 cgaagttgta cgccgggcgg ccgtcggatc gcagcagcac cgggtcgccg atcacgccgg
     1261 catcgaagcg caccgtgccg cggacgacgt cctcgaacgc cacgtcgcgt cgcgcgggca
     1321 cgcggaacct gaccgccgcc gcctcgcccg cggccacgcg ccgggcggcc tcggctggcg
     1381 gaagagcgcg gcacgcgccc gagtactgcg gcggccggcc cgcctcgagg gccgcctgcc
     1441 gctcgcgctc gagggcgtcc ggggaacaga agcagtagta cgcctggccc gcgtccagga
     1501 gttccccggc gtacgaccgg tacaggtcga ggcgctcgga ctgccggtac ggcccgtagg
     1561 cgcccccgac gtccggcccc tcgtcccact gcaggccgag ccagcgcagg tcatcgagga
     1621 tgccgcgctc ggactccggc gtcgagcgct cggcgtccgt gtcctcgatg cgcaggatga
     1681 acgtcccgcc gccgcccctg gcgaggagcc agttgaacag cgccgtgcgc gcgttcccca
     1741 cgtgcagcag gcccgtcgga ctcggcgcga accgcacccg catgagggac ccccgcctcc
     1801 tacccccgcg gcgacacgag cgccaccgcg tgggcggcca tcgcctcgcc gcgcccgacg
     1861 gcgtcgagcc cttcgttggt cttgcccttg acgctcacgc gcgccacggc gatgccgagc
     1921 gccccggcga ggcccgcgcg gatggcgtcg gcgtagggcg cgatcttcgg gcgctccgcg
     1981 acgatgacga cgtccacgtt cacgacctcg aacccggcgt cccgggcgac gccggcggcg
     2041 tgcgccagca tcgcgaggct cgacgcgccc ttccaccgcg ggtcggcgtc cgggaagtgg
     2101 cggccgatgt cgccgcccgc gatcgcgccg agcaccgcgt cggtgacggc gtgcgcggcg
     2161 acgtcggcgt cggagtggcc cgccaggccg cggtcgaacg ggatcgtgac gccgcccagg
     2221 acgagcgggc gcccctccac gaggcggtgc aggtcgtacc cggcgcccac gcgcgcgccg
     2281 ggccccccgg ccacggcgtc cgctgtcgtc aggtcggtca tggtcgtgat cttcatgttg
     2341 gaggcctcgc ccggcacgag ccggaccggg aagccggcgg cctccgcgag cgcggcctcg
     2401 tcggtggcct cgatgccgcg gccggcctcg atggccgcgg ccagcacctc ggtccggaac
     2461 gcctgcggcg tctgcgccag gtggatgcgc gcgcgcggga gcgtccgttc cacgaccgcg
     2521 cctcccgcag cgtcttcgcg cgccagcttg accgtgtcgc tcgccgcgac ggccgccagg
     2581 gccgcgcccg attcctctgc ggcctcgatc acgcgcgtga cgagcgagag gctggcgaac
     2641 ggcctggccg cgtcgtggat cacgacgagg tccgcccggc cggcgaccgc ctcgaacccg
     2701 cgccgcaccg agtcctgccg ccgcgcgccg ccggcgacca cgcggagcgg cttcgcgccc
     2761 gcgtgcaggc ccgccggcgg gtcggccgcc agctcggccg gcagcacgac cacgatctcg
     2821 tccaccaggt cgtggcccgc gaagatctcg accgaccgcg ccaggatcga cacgccgccg
     2881 acaggcagca gctgcttcgg cacgccggcg cccagccgtt cgccgcgccc gcccgccgcg
     2941 atgatcgccg ctacgcgcat ctcaggagac ccaccgcccc gtttccgcgc ccccagcgta
     3001 ctcggcgagg cgggcgcttg tcatgtcgag cacccgggcc atccgctcgc gcggcaccgc
     3061 ggcgggcgcg aacacggcga ccagcacgtc cctcgtcgcc gcccgcgtca tcgtccgggc
     3121 ggagtccgag gtcgggttgc cgaaggggcc gagcgcgtcg aagagcgcca ggcgcccgtc
     3181 gaggcgcacg gcgtctttgc ggattcccgc ataggcgtcg cccggcccgc cccgccgcag
     3241 cacgaggggc ggccggatcg cggccgcgtc gtagagcccg tagggcagct gcgtctgcag
     3301 tgagcaccaa ttggcgatgt ccgccagcgc gttgatgcgc gggaacggcc caccgcgcct
     3361 gacgcgccgg aggagggcct cggacgaggg ccgcgtcttc gtcgggtcca cgccgatggc
     3421 ccggtagagg gcgcgcgcgg cgctcgtgtc cgccacgtcg cccccgcgcg cggcgccgac
     3481 ggcctcggcg agcggcgcat cgagcgcgcg gtccgccccc ggggcgggcg ccacggcgca
     3541 ccacagcacg ccgagccgga cgatccgctc aaggccgggc tcgatcgcga cgccggcggt
     3601 ctcgtcgccc gtcatgccgc gcctgcgcgc cccagggcct cgacgccccc ggcgacaatg
     3661 gcccggacct ccgcggcgaa cgcgcgcacg tcctcgatgc cgggctccgc cgcccgcccc
     3721 cgcgtctcga tcggcggatg gacgacgagg gacacgacgc ccggccggac ggtgaactcg
     3781 cccttccgca tgacatcccg ggtgccggca atcgagagcg gcacgacggg cagcccggcc
     3841 tggagcgcca gggtgaagct gccggacttg aagcggcgca ccgtgccgtc ggcgctgcgc
     3901 gtcccctcgg ggaagatgat gagcgagagc ccctcgccga cgaggccgcg ccaccgccgc
     3961 aggatcccct cccggtccgg atgggcgcgg tccaccagga tgtgccccgc acggcgcagg
     4021 tgccagccga cgaacgggat tgggccgagg gactgcttgg cgatgatgcg gatctgataa
     4081 gggatcgatc cgaacaggac cggaatgtcg tacaggctct ggtggttcgc gacgaagacg
     4141 taggtggcgc ccgggacgag ggcgtcgagg ccgcgcacgc ggaccctgac ggcggcggcg
     4201 gcgagcacgc cccgccccca ccactgggcc agccggtggg ccgcgcggcc gctccgatcg
     4261 aagagggtgg tggcgagcgc ggccacaccc gctgcgacgg tcaggacgac gactgccggg
     4321 agcgtcacga ggagcgtgcg ccaccaggcc ggcgagggga gcgtcacggg tcctgcgccg
     4381 ccggcggcgg cggcggtcct gcgcgcgtcc ggcccctggc gtcctcgcgc tcgggcggcg
     4441 cctgcgggcc cgtgcccgcg ccgatgtgcc ggccgaagat catcttgccg gcggtggtct
     4501 ggagcacgct cgtcacggtg atgtcgatgg tccggttgag cagccggcgg gcattgtcca
     4561 cgacgaccat cgtcccgtcg tcgaggtagg cgaccccctg gttgtactcc ttgccctcct
     4621 tcaggatgag gaccgtcatc acctcgccgg gaaggaccac gggcttcacg gcgttggcga
     4681 gatcattgat gttcagcacc ccgaccccgc gcaggcgggc caccttgttc aggttcaggt
     4741 cgttggtcgc gatgcgggcg ttcagcgagc gcgccagctc gatcagcttg agatccacct
     4801 cgcggatgtc ggggaagtcc gtgtcggaga ccacgacgtc cacgccggcc atcttccgca
     4861 tccggtggag gatgtcgagg ccgcggcgcc cccggttcct tcgcatggtg tcggtggaat
     4921 ccgcgatctg ctggagctcg cggagcacga actgcggcac gacgagcgtg ccgtcgagaa
     4981 agccgacctc gcagacatcc gcgacgcgcc cgtcgatgac gacgctcgtg tcgaggaccg
     5041 tctggcggcg cgcggcggcg gagccgcgga agagggccgt cagccgggcc ggctgcagcc
     5101 actcgccgcg gcgcgcgccg atgatgaggc cgaggtaggg gaagagcagc aggatcacgc
     5161 gctggacgaa ggccccgtgc ccccgatcgg accacatgag cgcgccggtg accagcttcg
     5221 cgatgaacag cccgagggcc gcgccgatgg cggcccccag gatgcgcggc acgctgacgt
     5281 cgcgcatgcg ccattccagc gcgatcgcgg ccagggcgat ggccgcgccg agcgaggcgt
     5341 tcgcggcgag gcccgcgccc tcgaggggca gcagcacggc ggccgtgtag gtcacggccc
     5401 cgaggaagat cgcgcgggcg aggaggaacc acgccatgcc gcctcctgac actcctgcac
     5461 tataccgggg tcgggagggg ttcacgcaag cagatgctcg agcgcctccc cgacggtcct
     5521 gacgccggcc agggcgcaac cggcgtcggc gaaccccggg tccttccggt cgatgctggc
     5581 ggcgggcatg acgcaccgcg agaagcccat ctgggccgcc tcgcgcacgc ggcgcgcggc
     5641 ctgcgccgtc gcccggactt cgcccgccag cccgacctcg ccgaacaccg ccgtgccggc
     5701 ggcaatcggc cggttccgga agctcgacgc cacggccgcg acgaccgcca ggtcgagcgc
     5761 gggctcgtcc acgctcaggc ctcccgcgac gttcacgtac acgtcgtcgc ccccgagcgc
     5821 gagcccggcc cgcttctcga gcaccgccag cagcagcgag aggcggttct ggtccaggcc
     5881 cacggcggtc ctcttcgcgt agccgtacga gctgctgctg acgagcgcct ggatctccac
     5941 gagcagcggc cgcgttcctt cgatgcagca gagcacggcg gagccggggg cgttcgccgg
     6001 ccgctcggcc aggaacatcg ccgacgggtt gggcacgggg acgaggcccg ccgccgtcat
     6061 ctcgaacacg ccgagttcgc tcacggcgcc gaaccggttc ttggccgcgc ggacgatgcg
     6121 gtgcgcgtgc tggcgctcgc cttcgaagta cagcaccgtg tccacgatgt gctcgagggc
     6181 cttgggcccg gcgaggctgc cttccttggt gacgtggccc acgaggaacg tcggcacgtt
     6241 ctgcgccttg gcggcgaaca agagctgcgt ggcggcctcg cggacctgcc cgatgctgcc
     6301 gggggccgac tgcatcctga gcgagaacac cgtctggatc gagtccacga tcacgagcgc
     6361 cggcgaaagc ctcgtcatct cctcgaggac gcgctcgagg caggtctcgg ccagcaggta
     6421 gagcggcgcg tcgccgacgc cgaggcgctc gccccgcgat ttcacctggt gctcggactc
     6481 ctcgcccgag ctgtagagca cgggccctgc ggtgcgcgca agatgggccg cggtctgcag
     6541 cagcagcgtg gacttgccga tgcccggctc gccgccgagg agcacgagcg agcccggcac
     6601 gatgccgccg cccagcacgc ggtcgagctc gccgatgccc gtcgcaatgc gcgccgcgct
     6661 ggaggcctcg atgtcggcgt agagcctcgc gccgccgccg gcgggggccg ggtagcgtcc
     6721 cgccggcgcg gacgccggcg cgcgctcctc gaccagcgag ttccacgcgc cgcagtcggg
     6781 gcaccggccg agccacttgg gcgactgcgc cccgcactcc tggcaggcg
//
LOCUS       DAOSIF010000112         6813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10139_length_6813_cov_3.812814, whole genome shotgun sequence.
ACCESSION   DAOSIF010000112 DAOSIF010000000
VERSION     DAOSIF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10139_length_6813_cov_3.812814"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLT35_05300"
     CDS             <1..371
                     /locus_tag="PLT35_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28677.1"
                     /translation="LAAAAALAPSLILAQSVVQNALAVLFPAWVAGTGPRARGIDAMG
                     QRLLVMAGILVILLLSVLPGAVVAALVAAAAYRLTGAVPIVVPALVVSAVVAGECWLA
                     LEGLGRIFDRTDASAADAAG"
     gene            440..1003
                     /locus_tag="PLT35_05305"
     CDS             440..1003
                     /locus_tag="PLT35_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG28678.1"
                     /translation="MSRAARTPAARHQAHRTVVAMLRAADLLRRRTAAIVEPAGITTQ
                     QFNVLRILRGARAGGEDALPTLEVGSRMIEQTPGVTRLLDRLEAKGLVSRRRCPEDRR
                     RHRCRITPRGLGLLAALDDPILEDSRQALRRLTARQQAALAGLLDAVGEEQGGKQGQE
                     SRRQNSESRRQKTHDTGRKTSHVIRRT"
     gene            1142..1675
                     /locus_tag="PLT35_05310"
     CDS             1142..1675
                     /locus_tag="PLT35_05310"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOG28679.1"
                     /translation="MSTSRTFTIDKTHSEATFQVRHLVTKVRGHFSDLGGTIQADEAH
                     PERSSVTFTIQAASIDTGTPDRDAHLRAEDFFHVEKYPAITFVSTSIVAAGGDRYDVT
                     GDLTMRGVTKRITLPVTFLGSAKDPWGHEKIGFETETTLNRKDFGLTWNAALETGGLL
                     VGDEVKVSVSIQAAPVA"
     gene            complement(1736..1918)
                     /locus_tag="PLT35_05315"
     CDS             complement(1736..1918)
                     /locus_tag="PLT35_05315"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOG28680.1"
                     /translation="MKKNVGGVDRIVRGVAGSALVVWALAGGPVWAWIGVVPLVTAVL
                     AFCPLYAPFGISTCRK"
     gene            complement(2065..3714)
                     /locus_tag="PLT35_05320"
     CDS             complement(2065..3714)
                     /locus_tag="PLT35_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG28681.1"
                     /translation="MKRGWVGVLLAAACAAGQIAHGQAPEAGAAFVAPGPVEPLRPHV
                     MAALAGITRDAVAAHVRFLASAALEGRGLGARGLDAAAEYAAATLAIAGLEPVPQAEG
                     GIPAEPYFHPVPIREITRRGGHVLVERRRGDALESWTFAHGVDAVLPRLLPRVSSAPA
                     VYAEYGIRTASPSRDDYRDLDVRGRIVVVEDGVPKGAEWSRPALADRWASPDTDDRHE
                     AKRALAESLGARALVVIEREGFAAAAAASAAAPAFFAPFDAGEDPSSHVPVVRVSGRA
                     GRILLAGGAAPAGEPGGDVPRPLPGTVVSIAASGAERAIVSRNVIGMIRGSDPRLRDE
                     AVVVGAHLDHLGVRDGAVYPGADDNASGVASLLEMAKAFAAAPARPKRTVVVAFWTGE
                     EEGHLGSEHYAAHPAWPLARTSAYVNLDMIGHPWKAEEIAALVKEARLEHGAAFLAAV
                     KPADFVELGVADSAPHLEPVLARAARACGLALHLDRTNGRSGGSDYRAFARRGVPFVR
                     VFGNFFDGYHEPVDTADRLDAEQVAKVARLAFASAWLLADR"
     gene            complement(3774..4937)
                     /gene="iadA"
                     /locus_tag="PLT35_05325"
     CDS             complement(3774..4937)
                     /gene="iadA"
                     /locus_tag="PLT35_05325"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HOG28682.1"
                     /translation="MLLLKNADVYTPAAIGRADILLAGGRIVMVGPDIEVPARLCERV
                     DASGLVAAPGFIDGHVHLAGAGGEGGFATRTPELAFSDAVRGGITTVVGCLGTDGATR
                     SPAGLLAKARALDEEGLSTFVLTGHYAVPVQTLTGSIERDLLLIDKVVGVGEVAISDH
                     RSTQPTFEQFVRVAAEARRGGMLSGKAGVVNVHVGDGPRGLSMLRRAVEETEIPATQF
                     WPTHINRNPALFDEGQAWAKAGGVVDFTTSTVPAFLDAGEVRCGAGLRRMLEAGVDAS
                     RVTFTSDGQGSLPDYDAAGELRGLRVGRVTSLAASVRDAVLVEGVPLASALLAVTANP
                     ARILKLRGKGALGAGADADLVLLDPAGLEIDGVIAKGRWLMKGREIVVRGTFE"
     gene            5195..5617
                     /locus_tag="PLT35_05330"
     CDS             5195..5617
                     /locus_tag="PLT35_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG28683.1"
                     /translation="MAAKTVLLVDDDLDLLDIYRITLEGEGFDVLTAENSDEAMRIAT
                     TTPIDAAVLDVMMRTPTEGFLLARALRQDERTRRIPLLMLTSVNAENEAQGYHRRFSD
                     RDRDDQWLPVDRFVDKPIKPEDLVTTVRTLCGGWTFTK"
     gene            5647..>6813
                     /locus_tag="PLT35_05335"
     CDS             5647..>6813
                     /locus_tag="PLT35_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG28684.1"
                     /translation="MISPADDASPVPRGRGRSFVEQELANSLGWLISLRWVAGAAVVL
                     ASVVVRVALPEQLPAAPILLVGAGILLYNLVLSWVLAWLQRSRPEATSGFEYLVRAQV
                     ALDWGAMTVLVASSGGIESPAISFFLFHITISSLLLPHQHVFLYVTLAPVLVGGVAVL
                     EYAGVLPHVSLVQAARYRDPLYVGAVLVFFATSCYVMAYFCVSIAKRLRRREAELKGL
                     YDGVRDVTSTLDITAVLERIVEAAARVLTCKAAAIRLIDQSHSQVEFAASWGLSDTYR
                     DEVPAEYARSTLDQDTLRSGVVHVTDVGGDPRVFHPELARDERIASMLSVVIKGRTGP
                     IGVLRAYGAPGHVFNAEDVAYLQAMAAHGAVAIENAKAYRLLADLDRDKSKFLRI"
BASE COUNT          966 a         2531 c         2429 g          887 t
ORIGIN      
        1 acctcgcggc cgccgcggcg ctcgctccgt cgctcatcct cgcgcagagc gtcgtgcaga
       61 acgcgctggc ggtgctgttc ccggcctggg tcgccggcac cgggccgcgc gcgcggggaa
      121 tcgacgccat gggtcagcgg ctgctcgtga tggccggcat tctcgtgatc ctgctgctgt
      181 cggtcttgcc cggggccgtc gtggcggcgc tcgtggcggc agccgcctac cggctcaccg
      241 gcgccgtgcc gatcgtggtg ccggcgctgg tcgtgtcggc cgtcgtcgcg ggggagtgct
      301 ggctggccct cgaggggctc ggacggatct tcgaccggac ggacgcctcg gccgccgacg
      361 ccgccgggta gggggctccg gccgcccggc gccggcgcaa atacttgaca tgacaactat
      421 tgactggtag tctaatggcg tgagcagggc ggcgcggacc ccggcggcga gacaccaggc
      481 ccaccggacc gtcgtggcca tgctccgggc ggccgatctc ctgcgccgcc ggacggccgc
      541 catcgtcgag ccggccggaa tcaccacgca gcagttcaac gtgctgcgga tccttcgggg
      601 cgctcgcgcc ggcggcgagg acgccctgcc gacgctggag gtcggaagcc ggatgatcga
      661 gcagaccccc ggcgtgaccc ggctcctcga ccgcctcgag gccaaggggc tggtgagccg
      721 ccggcgctgc cccgaggacc gccgccggca ccggtgccgg atcacgccgc gcgggctcgg
      781 cctcctcgcc gccctcgacg acccgatcct cgaggacagc cggcaggccc tgcgccggct
      841 gaccgcgcgc cagcaggccg cgctcgccgg cctgctcgac gccgtcggag aggagcaagg
      901 cgggaagcag ggacaggaat ccagaagaca gaattcagaa tccagaagac agaagacgca
      961 cgacacaggg cgcaagacgt cacacgtcat acgtaggacg tgagcggcaa gacgtcaggc
     1021 gcacgacttg agacctgaga cgcagaacgt cagacgcaag acgcaagacg acacggaacc
     1081 cggggcgtgg gccccggcag gcacaggacc cggacaccgc cagactgctg cgaggacaac
     1141 gatgagcacg agccgcacgt tcacgattga caaaacgcat tccgaagcga cgttccaggt
     1201 ccgccacctc gtcacgaagg tgcgcgggca cttctccgac ctcggcggca cgatccaggc
     1261 cgacgaggcg cacccggagc gatcctccgt cacgttcacc atccaggctg ccagcatcga
     1321 cacgggcacg ccggaccggg acgcgcacct gcgggcggag gacttcttcc acgtggagaa
     1381 gtacccggcc atcaccttcg tcagcacgtc aatcgtcgcc gcaggcggcg accgctacga
     1441 cgtgaccggc gacctgacga tgcgcggcgt cacgaagcgg atcacgctgc cggtcacgtt
     1501 cctcggctcg gccaaggacc cgtggggcca cgagaagatc ggcttcgaga ccgagacgac
     1561 gctgaaccgg aaggacttcg ggctcacgtg gaacgcggcc cttgagaccg gggggctcct
     1621 ggtgggagac gaggtgaagg tgtcggtgtc gattcaggcg gctcccgttg cgtgagcgga
     1681 cgccgactga ctggcgtgcg gcgcggcccc ggctggagcc ggggccccgt cgctcctact
     1741 tcctgcaggt cgagatcccg aacggcgcgt agagcgggca gaaggcgagc accgccgtca
     1801 cgagcggcac cacgccgatc caggcccaga cggggccgcc tgccagcgcc cacacgacca
     1861 gcgccgaacc cgccacgccg cgaacgatcc gatcgacgcc gccaacattc ttcttcatcg
     1921 aacggccctc cagatccagg tggacgacag gggcccgacg gggcctctgc cttggagaga
     1981 ttcacgggcc ggccgtcggt cacagccggc cgttccgccg ccgcgagcgg gctgctgcgc
     2041 cgggacggcc tcggcggccg ggtgtcagcg gtccgccagc agccacgccg acgcgaacgc
     2101 cagccgcgcc accttggcca cctgctcggc gtccaggcgg tcggccgtgt cgacgggttc
     2161 gtggtagccg tcgaagaagt tcccgaagac gcgcacgaac ggcacgcccc gccgcgcgaa
     2221 cgcccggtag tcgctgccgc cgctgcgtcc gttcgtccgg tcgaggtgca gcgccaggcc
     2281 gcacgcgcgg gcggcccggg cgagcacggg ctcgaggtgg ggagcggagt ccgcgacgcc
     2341 gagctcgacg aagtccgccg gcttcacggc cgcgaggaag gccgccccgt gctcgaggcg
     2401 cgcctccttc acgagcgccg cgatctcctc tgccttccac ggatggccga tcatgtcgag
     2461 attgacgtag gccgacgtgc gggcgagggg ccacgccggg tgcgcggcgt agtgctccga
     2521 cccgaggtgg ccttcctcct cgccggtcca gaacgccacg accacggtgc gcttcggccg
     2581 cgccggcgcg gccgcgaagg ccttcgccat ctccaggagc gaggcgacgc ccgacgcgtt
     2641 gtcatcggcc ccgggataga ccgcgccgtc ccgcaccccc aggtgatcga ggtgcgcacc
     2701 gacgaccacg gcctcgtccc gcagccgcgg atccgagccg cggatcatgc cgatcacgtt
     2761 gcggctcacg atcgcgcgct cggctccgct tgccgcgatc gacacgaccg tgcccggcag
     2821 cggcctcggg acgtcgccgc caggttcgcc ggcaggcgcc gcgccgccgg cgagcaggat
     2881 gcgcccggcg cgccccgaca cgcggacaac cggcacgtgc gacgaagggt cctcgccggc
     2941 gtcgaacggc gcgaagaacg caggggcagc cgccgacgcg gcggcggcgg ccgcgaaccc
     3001 ttctcgctcg atgacgacga gcgcccgcgc gccgagcgac tccgccagcg cgcgtttcgc
     3061 ctcgtggcgg tcgtcggtgt cggggctcgc ccagcggtcg gcgagcgcgg ggcgcgacca
     3121 ctccgccccc ttcggcacgc cgtcctcgac gacaacgatg cggccgcgga cgtcgagatc
     3181 ccggtaatcg tctctcgacg ggctggctgt gcggatgccg tactcggcgt agacggccgg
     3241 cgcggagctg acgcgcggca gcagcctggg cagcacggcg tcgacgccgt gagcgaacgt
     3301 ccacgactcg agcgcgtccc cgcgccggcg ctccacgagc acgtggccgc cgcgccgcgt
     3361 gatctcgcgg atcgggaccg ggtggaagta cggctccgcc ggaatgccgc cctcggcctg
     3421 cggcaccggc tccagtcccg cgatcgccag cgtcgccgcc gcgtactcgg cggcggcgtc
     3481 gagtccgcgc gcgccgaggc cgcgcccttc gagcgccgcc gaggcaagga agcgcacgtg
     3541 cgccgcgacg gcgtccctgg tgatgcccgc aagcgccgcc atcacgtgcg gccgaagcgg
     3601 ttcgaccggg cccggggcga cgaacgccgc tcccgcctcc ggcgcctgtc cgtgcgcgat
     3661 ctggccggcc gcgcacgcgg cggccaacag cacgccgacc cagcctcgtt tcatggcttt
     3721 gcgccccccc ccgtgacggg cgcgcctccg gcgccgccgg ccgatgcgcg cgcctattcg
     3781 aaggtcccgc ggacgacgat ctcgcgcccc ttcatcagcc agcggccctt tgcgatgacg
     3841 ccgtcgatct cgagccccgc cgggtcgagc agcaccaggt cggcgtcggc gcccgcgccg
     3901 agcgcgccct tgccgcgcag cttgaggatg cgcgcagggt tcgccgtgac ggccagcagg
     3961 gccgaggcca gcggcacgcc ctcgacgagg accgcgtcgc ggaccgatgc ggcgaggctc
     4021 gtcacgcggc cgacccgcaa cccgcgaagc tcgccggccg cgtcgtagtc cggcaagctg
     4081 ccctggccgt cagaggtgaa cgtcacccgc gaagcgtcca cgccggcctc cagcatcctc
     4141 cgcagccccg cgccgcaccg cacttcgccc gcgtcgagga aggccggcac ggtcgaggtc
     4201 gtgaagtcca cgaccccccc ggccttcgcc cacgcctggc cttcgtcgaa cagcgccgga
     4261 ttgcggttga tgtgcgtcgg ccagaactgg gtcgcaggga tctcagtctc ttcgacggcg
     4321 cgcctgagca tcgacagccc gcgcgggccg tcgcccacgt ggacgttcac gacgccggcc
     4381 ttgcctgaga gcatcccgcc gcgccgggcc tccgcggcca cgcgcacgaa ctgctcgaag
     4441 gtcggctgcg tggagcggtg gtcggaaatc gccacctctc cgacgccgac caccttgtcg
     4501 atgagcagga ggtcgcgctc gatgctgccc gtgagcgtct ggaccggcac ggcgtagtgt
     4561 cccgtcagga cgaacgtgct caggccctcc tcgtcgagcg cgcgggcctt cgcgagcagg
     4621 cctgcgggcg accgcgtggc cccgtcggtg cccaggcagc cgacgaccgt ggtgatgccg
     4681 ccgcggaccg cgtcggagaa cgcgagttcg ggcgtgcgcg tcgcgaagcc gccctcgccg
     4741 ccggcgccgg ccagatgcac gtgcccgtcg atgaagccgg gcgcggccac cagcccggac
     4801 gcgtcgaccc gctcgcagag gcgggcgggc acctcgatgt ccggtccgac catgacgatg
     4861 cggccgccgg ccaagagaat gtcggcgcgg ccaatcgcgg cgggggtgta gacgtcggcg
     4921 ttcttgagca gcagcacggg aaccagtcta cctcggcggc cgcgccggag cgcacgcgcg
     4981 cggaggcgcg ggccggccgc cgcgctcctg cggacttgcg gaattcgcgc ggactggcga
     5041 tgatgctccg gatcgcgtgc gcgggcgcgc cggcgggtcc ggcggccggc tggcgcgtgc
     5101 gccttgccgg ggcctcgcgt cgccgcccgc gcggggacgg ccgcgcgacg gcccctcacg
     5161 cccccgggtg accggcgcgc agggaggacg ggtgatggct gcgaaaaccg tgctgctcgt
     5221 ggacgacgat ctggacctgc tcgacatcta ccggatcacg ctcgagggcg aggggttcga
     5281 cgtactgacg gccgagaact cggacgaggc gatgcgcatc gccaccacca ctccgatcga
     5341 cgcagccgtg ctcgacgtga tgatgcggac gccaacggag ggcttcctgc tggcgcgggc
     5401 gctccggcag gacgagcgca cgaggcgcat cccgctgctg atgctcacct cggtgaacgc
     5461 cgagaacgag gcccagggct accaccgccg gttctcggat cgcgaccgcg acgaccagtg
     5521 gctgccggtg gaccgcttcg tggacaagcc catcaagccg gaggacctcg tcacgacggt
     5581 gcgcaccctc tgcggcggct ggaccttcac gaagtagcgg cccgtccgtt cccggtggaa
     5641 gggaagatga tctcacccgc cgacgatgcg tctcccgtcc cgcgcgggcg cggccgatcc
     5701 ttcgtcgagc aggagctcgc gaacagcctc ggctggctga tctccctccg ctgggtggcc
     5761 ggcgccgccg tcgtcctcgc ctccgtggtg gtgcgggtgg cgctgccgga gcagcttccc
     5821 gccgcgccga tcctgctcgt gggcgccggc atcctgctct acaacctcgt cctgtcgtgg
     5881 gtgctcgcct ggctgcagcg ctcgcggccg gaggccacga gcgggttcga gtacctcgtg
     5941 cgcgcgcagg tcgccctcga ctggggggcc atgacggtcc ttgtcgcctc gtccggcggg
     6001 atcgagagcc cggccatcag cttcttcctg ttccacatca cgatctcgtc gctgctgctg
     6061 ccgcaccagc acgtcttcct gtacgtcacg ctggccccgg tgctcgtggg cggcgtggcg
     6121 gtgctcgagt acgccggcgt cctgccgcac gtgtccctcg tccaggcggc gcggtaccgc
     6181 gacccgctgt acgtcggcgc cgtcctggtg ttcttcgcga cgtcgtgcta cgtgatggcg
     6241 tacttctgcg tgtcgatcgc gaagcgcctg cgccgccggg aggccgagct gaagggcctc
     6301 tacgacggcg tgcgcgacgt gacctcgacg ctggacatca cggccgtgct cgagcggatc
     6361 gtcgaggccg ccgcgcgcgt gctcacgtgc aaggccgctg ccatccgcct catcgaccag
     6421 agccactcgc aagtcgagtt cgccgccagc tgggggctga gcgacacgta ccgggacgag
     6481 gtgcccgccg agtacgcgcg ttcgacgctc gaccaggaca ccctgcgcag cggcgtcgtg
     6541 cacgtgacgg acgtcggcgg cgacccgcgg gtcttccacc ccgagctcgc ccgcgacgag
     6601 cgcatcgcct cgatgctcag cgtggtcatc aagggccgca ccgggccgat cggcgtgctg
     6661 cgcgcgtacg gcgccccggg ccacgtcttc aacgccgaag acgtcgccta cctgcaggcg
     6721 atggcggccc acggcgccgt ggccatcgag aacgccaagg cgtaccggct gctggccgat
     6781 ctcgaccggg acaagtcgaa gttcctccgg atc
//
LOCUS       DAOSIF010000113         6788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10181_length_6788_cov_3.422843, whole genome shotgun sequence.
ACCESSION   DAOSIF010000113 DAOSIF010000000
VERSION     DAOSIF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10181_length_6788_cov_3.422843"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PLT35_05340"
     CDS             <1..1101
                     /locus_tag="PLT35_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG28685.1"
                     /translation="TGGMLVAARVIQGMGGGMVFGTATAMLISVYPRTERGRVLGWSV
                     ASVYLGLSLGPPAGGWIVGQFGWRAIFWTNACLCACSLGVTLRHLRAEWKEAEGEAFD
                     VAGAVAYGLSVVALMLGLAVLPSIRGGALLALAVAGIGLFVRWEARSASPLLDVRLLR
                     ENRVFAFSNLAALINYAATFAVGFVLSLHLQYVKGLTPQQAGLVLMSQPVMQALFSPL
                     AGRLSDRVEPRLLASAGMALTAAGLASLSFLGAGTSLAHVGASLFVLGVGFGLFSSPN
                     TNAIMGSVDRRQYGLASAVLSTMRMLGQMFSMGMAALVIAVFVGRAAIGPSNQDAFVR
                     GARLLLGAFAALSAFGVFASLSRGTIHTSRRG"
     gene            1267..1977
                     /locus_tag="PLT35_05345"
     CDS             1267..1977
                     /locus_tag="PLT35_05345"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOG28686.1"
                     /translation="MINGQGAARGGLNEDGRRRIRRVAAMMGVIALAMFVPAGRIGWV
                     QGWVYVGLYAAAVIAGGSWVVRRNPAAINARGRRDGATARFDRVLAPLMLGIGLAQYV
                     VAGLDERFGWSSVPAPLQALGAAGLLASMAGAFWAMASNPFLATTVRIGEDGSHRVAT
                     AGPYRIVRHPMYASSVWFAWATGCLLDSWWAVLCSALSAVLFIVRTALEDRALVAGLE
                     GYREYAARVRYRLAPGVW"
     gene            2066..2689
                     /locus_tag="PLT35_05350"
     CDS             2066..2689
                     /locus_tag="PLT35_05350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG28687.1"
                     /translation="MRTPLAWLAVAQRIQAISQTGLAFAANEYDIGRYRELSRIAAAM
                     MAGPEPEPVALAEGVFALERGYATPKVDVRAAVCREGGLLLVRERDDGGWTLPGGWAD
                     VGQSAAECVEREVREESGYLVKAVRLLACWDRNRHGHTPHPFHVYKLVFQCELVGGAP
                     AASEETSDVGFFPEDRLPELSRTRIVPEQIRFVFAALRDPAMPPAFD"
     gene            complement(2753..3364)
                     /locus_tag="PLT35_05355"
     CDS             complement(2753..3364)
                     /locus_tag="PLT35_05355"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOG28688.1"
                     /translation="MRTRLVAAAVALAATAAPVGAIAAPVGAPAPAFKGTDTKGVTRS
                     LSDFAGKWVVLEWHNSGCPYVRKHYGGHNMQALQREWTSRGVVWLTVISSAAGQQGHM
                     EPAQADAYFADQKASQTAVLLDPSGEIGRLYDAKTTPHMFVINPDGVLVYNGAIDDRP
                     TTDLADLSGATNYVSAALTEGMAGKAVTVATSRPYGCGVKYAR"
     gene            complement(3408..4283)
                     /locus_tag="PLT35_05360"
     CDS             complement(3408..4283)
                     /locus_tag="PLT35_05360"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOG28689.1"
                     /translation="MTLNRAAVLCAAAAAASGAAIAARAHPAGPPGRTGQGPHVRVEL
                     IADRATPAPGMRLGLKFDLDPGWHIYWLNPGDSGEPPEARWTLPPGLAASGFEWPAPE
                     RIDAGGLVNYGYHGGVVLPSTIAAAAPGGAAPPGAFTAGASVRWMVCADMCVPGRAEL
                     ALAFPLAAQERARVAAWKAAIDEARSRVPAPAPASWKASARSAAGEFVLEVATGSREA
                     EAVFFPLDASQVNDSAAQAVTPLASGVRVTLRKSSQLVKDPAVLKGVLALAGRRAHVV
                     EARVRSSDSGFQQPK"
     gene            complement(4415..6334)
                     /locus_tag="PLT35_05365"
     CDS             complement(4415..6334)
                     /locus_tag="PLT35_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOG28690.1"
                     /translation="MSALDIVVFAAFVATVIGVGLYKSRGEATRSEQGAANYFLAGRG
                     LSWWLIGFSLIAANISTEQFVGMSGQAANWLGIAIASYEWMAAITLVVVAFVFLPTFL
                     RSGIYTMPEFLEYRFNPFARTIMALCTMVILVGVPTASVIYSGAKVISVYFAGITLFG
                     LDFGNIVVGCWIIGILSAVYVFAGGLKACAWADLLQGTALIAGGAVVMVMALRALGAA
                     DPLALVQTAVAPGADAAQIAQAGPWERIQLLNGGALPAGKLHMVRPGNDALIPWTALV
                     VGLWIPNFFYWGLNQYITQRTLGARTLNQGQRGIVFAAFLKLLIPFIVVFPGIMAFNL
                     FNGDMRQLGAEKNRPVLEAFARGEAQVYAFDEHFAALRPDMARSMFAANAAAVGAPGA
                     AAAPAEEPAALAAANDALVAAAQSAGRKVSTRLIGYDYDAAFPLLLKLLPQNGLLGFV
                     LVALFGAVVSSLAAMLNAASTIASMDIYRKIRKNATQYELVTSGRVFIVVFVLIAILI
                     APELGRPEFGGIFTFIQEFQGFISPGILAIFLFGLLVHRAPRVCGTVGLLLNPVLYGT
                     LKIAAPHVAFLDRMAICFAAVLAVLAVITLVKPMPKPVELPVNPSMDMRVDRSTKILG
                     GAVVVLTLLLYVVFW"
BASE COUNT          919 a         2456 c         2480 g          933 t
ORIGIN      
        1 acgggcggga tgctcgtcgc cgcccgcgtc atccagggca tgggcggcgg catggtcttc
       61 gggaccgcca ccgccatgct catctcggtg tacccgcgga ccgagcgcgg ccgagtgctc
      121 ggctggagcg tcgcctccgt ctacctcggg ctctcgctcg ggccgccggc gggcgggtgg
      181 atcgtcggcc agttcggctg gcgcgccatc ttctggacga acgcgtgcct gtgcgcctgc
      241 tcgctcggcg tcacgctccg ccaccttcgc gccgagtgga aggaagccga aggcgaggcg
      301 ttcgacgtcg ccggggccgt cgcctacggc ctctccgtcg tcgcgctcat gctgggcctc
      361 gccgtgctgc cgtccattcg cggcggcgcc ctgctcgcgc tggcggttgc cggcatcggc
      421 ctgttcgtcc gctgggaagc caggagcgcc tcgccgctcc tcgacgtgcg cctcctccgc
      481 gagaaccggg tgttcgcctt ctcgaacctc gcggccctca tcaactacgc cgcgacgttc
      541 gccgtgggct tcgtcctcag cctgcacctg cagtacgtga agggcctgac gccgcagcag
      601 gccgggctgg tgctgatgtc gcagccggtc atgcaggcgc tcttctcgcc gctggcgggg
      661 cggctgtccg accgcgtcga gccgcgcctg ctcgcctcgg ccggcatggc cctgactgcc
      721 gccggcctgg cgtcgctctc gttcctcggc gcggggacga gcctggcgca cgtcggcgcg
      781 agcctgttcg tcctgggcgt cggcttcggc ctgttctcgt cgccgaacac gaacgcgatc
      841 atgggctcgg tggaccggcg gcagtacggc ctcgcctccg ccgtgctcag cacgatgcgg
      901 atgctcggcc agatgttcag catgggcatg gccgcgctcg tcattgccgt cttcgtggga
      961 cgcgccgcga tcggcccctc gaaccaggac gcgttcgtcc ggggcgcgcg cctgctcctc
     1021 ggcgcgtttg ccgcgctctc ggccttcggc gtcttcgcgt cgctcagccg gggcacgatc
     1081 cacacctcca ggcgcgggtg aggcgatggc acgctccaat gccgcccccc gcgccatggc
     1141 gcccgccctc ggcggcccgg ccgccgctcc gacgagcgcc agcctggcgg ggctcggccg
     1201 cgcccgccag gcgctgtcgc cagcccgggg cgcacgacgg gaccgcccgt cgccgcggag
     1261 gccgcggtga tcaacggaca gggagcggcg cggggcggat tgaacgaaga cggccggcgc
     1321 cggatcaggc gggtcgccgc catgatgggc gtgatcgccc tcgcgatgtt cgtccccgcc
     1381 gggcggatcg gctgggtgca gggctgggtg tacgtggggc tctacgcggc cgccgtgatc
     1441 gccggcggct cctgggtggt ccgccggaac cccgccgcga tcaacgcgcg cggccggcgg
     1501 gacggggcca ccgcgcgttt cgaccgcgtg ctcgcgcccc tcatgctcgg catcggcctg
     1561 gcccagtacg tcgtcgcggg cctggacgag cgcttcgggt ggagcagcgt cccggcgccg
     1621 ctgcaggccc tcggcgccgc cgggctcctc gcgtcgatgg cgggcgcctt ctgggcgatg
     1681 gccagcaatc cgtttctcgc caccacggtg cgcatcgggg aggacggcag ccaccgcgtc
     1741 gccacggccg ggccttaccg gatcgtgcgg cacccgatgt acgcgtcgag cgtctggttc
     1801 gcgtgggcca ccgggtgcct gctcgactcg tggtgggcgg tgctgtgctc ggccctgagc
     1861 gccgtgctct tcatcgtcag gacggcgctc gaggaccgcg ccctcgtcgc cggactggaa
     1921 gggtatcgag agtacgcggc gcgcgtccgc taccggctcg cgccgggggt gtggtaggcg
     1981 gagggagggc gctcgcgcgc cgggcccggg gcggccggcc ggcgccggcg ggccggaacc
     2041 agtccccgtg gtacgatcgc ggcccgtgag gacaccactt gcctggctcg ccgtcgccca
     2101 gcgcatccag gccatctccc agaccgggct ggccttcgcc gcgaacgagt acgacatcgg
     2161 gcgctaccgt gagctctcgc ggattgcggc cgccatgatg gccggacccg agcccgaacc
     2221 cgtcgcgctg gccgagggcg tgttcgcgct ggagcgcggg tacgccacgc cgaaggtgga
     2281 cgtgcgcgca gcggtctgcc gcgagggcgg cctgctgctc gtgcgcgagc gcgacgatgg
     2341 cggctggacc ctcccgggag gatgggcgga cgtcgggcag agcgccgcgg agtgcgtcga
     2401 gcgggaagtc cgcgaggagt cgggctacct cgtcaaggcg gtgcggctgc tggcgtgctg
     2461 ggaccgcaac aggcacgggc acacgccgca cccgttccac gtctacaagc tggtgttcca
     2521 gtgcgagctg gtcggcggcg cgcccgcggc cagcgaggag acctcggacg tcgggttctt
     2581 cccggaggac aggctgccgg agctgtcgcg cacgcgcatc gtgcccgagc agatccggtt
     2641 cgtcttcgcc gcgctgcgcg atcccgcgat gccgcccgcg ttcgactgag cgggcctgcg
     2701 gacggagcgc gcgccgccgg acgaaggctc cggcggcgct tcggggcgaa ggctaccgcg
     2761 cgtacttcac gccgcagccg tacggcctcg acgtggcgac ggtcacagcc ttgcccgcca
     2821 tcccctcggt cagtgccgcc gacacgtaat tggtcgcgcc ggagaggtcc gcgagatcgg
     2881 tcgtcggcct gtcgtcgatg gcgccgttgt agaccagcac gccgtcgggg ttgatgacga
     2941 acatgtgcgg cgtggtcttc gcgtcgtaga gacgcccgat ctcaccggac ggatcgagca
     3001 ggaccgccgt ctgcgaggcc ttctgatcgg cgaagtaggc gtccgcctgc gcgggctcca
     3061 tgtggccctg ctggccggcg gccgacgaga tgaccgtcag ccacaccacg cccctggacg
     3121 tccactccct ctggagcgcc tgcatgttgt gtccgccgta gtgcttcctc acgtacgggc
     3181 agccgctgtt gtgccactcc agcacgaccc acttgcctgc gaagtccgac aggctgcgcg
     3241 tgacgccctt cgtgtcggtc cccttgaacg ccggcgccgg ggcgccgacg ggggcggcga
     3301 tcgcgccgac gggggcggct gttgcggcaa gggccaccgc cgcggcgacg agacgggttc
     3361 tcatggcgac tccttctctg gttgcggtgt tggtccgaac ggctgcgtca cttcggttgc
     3421 tggaatccgg agtccgagct ccgcactcgc gcctccacga cgtgcgcccg ccggccggca
     3481 agcgcgagga cgcctttcag cacggccggg tccttcacca gctggctcga cttccggagc
     3541 gtgacgcgca ccccggaggc gaggggcgta acggcctgtg cggccgagtc gttcacctgg
     3601 ctggcgtcga gcgggaagaa cacggcctcc gcctcgcgcg agcccgtcgc gacctcgagc
     3661 acgaactcgc cggcggccga ccgggccgag gccttccacg acgccggggc gggcgccggc
     3721 acgcggctcc tggcctcgtc gatggcggcc ttccaggctg ccacccgcgc ccgctcctgc
     3781 gcggcgagcg gaaacgccag cgcgagttcc gcgcggcccg gcacgcacat gtccgcgcag
     3841 accatccagc ggacggaggc gccggcggtg aacgccccag gcggcgccgc gccgcccggc
     3901 gccgcggccg cgatcgtcga cggcaacacc acgccgccgt gatagccgta gttgacgagg
     3961 ccgccggcgt cgatgcgctc aggcgccggc cactcgaacc cggaggccgc caggccaggc
     4021 gggagcgtcc accgggcctc gggcggctct ccggaatcac ccgggttgag ccagtagatg
     4081 tgccagcccg ggtcgaggtc gaacttcagg ccgagcctca tgcccggggc cggcgtcgcg
     4141 cggtccgcaa tcagctcgac gcgcacgtgc gggccctgtc ccgtccggcc cggagggccg
     4201 gccggatggg cgcgggccgc aatggcggcg cctgacgcag cggccgccgc ggcgcacagc
     4261 acggccgccc ggttgagggt catcgaaggc atgggaatcc cgacgtgccc tttggacgcc
     4321 cgggccagtc gcgaggatcc cggggacgat cgggacgagc ggccccctgg cgcgatcccc
     4381 gaagaccggg agcgcggcct ggcggcggcc ggcgctacca gaacaccacg tagagcagca
     4441 gcgtcagcac gacgacggcg ccgccgagga tcttcgtgct ccgatccacg cgcatgtcca
     4501 tcgacggatt gaccgggagc tcgacgggct tcggcatggg cttgacgagc gtgatgacgg
     4561 cgagcaccgc gaggaccgcc gcgaagcaga tggccatgcg atcgaggaac gcgacgtgcg
     4621 gcgcggcaat cttgagcgtg ccgtacagga ccgggttcag cagcaggccg acggttccgc
     4681 acacccgcgg ggcgcggtgc accagcaggc cgaagaggaa gatcgccagg atgcccggcg
     4741 agatgaagcc ctggaactcc tggatgaacg tgaagatgcc cccgaactcc ggccggccga
     4801 gctcgggggc gatgaggatc gcgatgagca cgaagaccac gatgaagacg cggcccgagg
     4861 tcaccagctc gtactgcgtc gcgttcttcc ggatcttccg gtagatgtcc atcgacgcga
     4921 tggtggacgc cgcgttcagc atggcggcga gcgagctcac cacggcgccg aacagcgcga
     4981 ccagcacgaa cccgagcagg ccgttctgcg gcagcagctt cagcaggagg ggaaacgccg
     5041 cgtcgtagtc gtagccgatc aggcgcgtgg acaccttccg cccggccgac tgcgccgccg
     5101 cgaccagcgc gtcgttggcg gccgcgagcg cggcgggctc ctccgccggc gcggccgccg
     5161 cgcccggcgc ccccacagcg gccgcgttcg ccgcgaacat cgatcgcgcc atgtccggcc
     5221 gcagcgccgc gaagtgctcg tcgaaggcgt agacctgcgc ctcgccccgg gcgaacgcct
     5281 cgaggacggg gcggttcttc tccgcgccga gctggcgcat gtctccgttg aacaggttga
     5341 acgccatgat ccccgggaag acgacgatga aggggatgag gagcttgagg aaggcggcga
     5401 acacgatgcc gcgctggccc tggttcaagg tccgcgcgcc cagcgtgcgc tgggtgatgt
     5461 actggttgag gccccagtag aagaagttgg ggatccacag cccgaccacc agcgccgtcc
     5521 acgggatcag cgcgtcgttg cccgggcgca ccatgtggag cttgcccgcc ggcagcgccc
     5581 cgccgttcag cagctggatg cgctcccacg ggcccgcctg cgcgatctgc gccgcgtccg
     5641 cgccgggcgc gaccgcggtc tgcacgaggg ccagcgggtc ggccgcgccg agcgcccgca
     5701 gcgccatcac catcacgacg gcgccgccgg cgatgagcgc cgtgccctgc agcaaatcgg
     5761 cccacgcgca ggccttcagg cctcccgcga agacgtagac ggccgacagg atgccgatga
     5821 tccagcaccc caccacgatg ttcccgaagt cgaggccgaa gagcgtgatg cccgcgaagt
     5881 acacgctgat gaccttggcg cccgagtaga tgaccgacgc cgtcggcacg cccacgagga
     5941 tcaccatcgt gcagagcgcc atgatggtcc gcgcgaacgg gttgaagcgg tactcgagga
     6001 actccggcat cgtgtagatg ccgcttcgca ggaacgtcgg caggaacacg aacgcgacga
     6061 cgacgagcgt gatggccgcc atccactcgt agctggcgat ggcgatcccg agccagttcg
     6121 ccgcctggcc gctcatcccc acgaactgct cggtggagat gttggccgcg atgagcgaga
     6181 acccgatgag ccaccacgac aggccgcggc ccgcaaggaa gtagttggcc gccccctgct
     6241 cgctgcgcgt cgcctcgccc cggctcttgt acaggccgac gccgatgacg gtggcgacga
     6301 aggccgcgaa caccacgatg tcgagtgcgc tcatcgggac cctccagggc gccgcttcgg
     6361 cggcgcccgg cacacggatt ctaccggttc tcccccggcg gcgcgcaaac gcccgggcgc
     6421 cggcgtccgt ccctggcagg aggcagcgcc gccgggaggg gcggaggaag ccggacacgc
     6481 gcccggctcc gggccgggcc gcttcgccgt gcagcggggc acgccctgcc gctgccgccc
     6541 tgccgctgcc gccgggcggc ggacaggagc ggcaacagcc ggtttccggt ccgccggcgg
     6601 gagatgaagg ctgccgccgc cacggcagcg ctataatcgt gccggcatcc ggcatcatcg
     6661 ggccccgaag atgcgccgtg cccgcggagg agcggcatga agaagacagg aatccgtcgg
     6721 agcggccccg cggccgcgat cgtgatggcg tctcttgcgg cactggcgtt cgcgcaggca
     6781 cccgccgg
//
LOCUS       DAOSIF010000114         6778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10198_length_6778_cov_4.090287, whole genome shotgun sequence.
ACCESSION   DAOSIF010000114 DAOSIF010000000
VERSION     DAOSIF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10198_length_6778_cov_4.090287"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="PLT35_05370"
     CDS             complement(<1..1798)
                     /locus_tag="PLT35_05370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG28691.1"
                     /translation="MLPGGDARRDQALEMALREVLPDQAVRERPDQPDGLVTAVRRVP
                     ARDADFEDLPPLVDPRLRDALARRGIDRLYSHQARAVAHALAGRHVVVTTPTASGKTL
                     CYNIPVMNAVLADPSSRALYLFPTKALAQDQLAELDRLTREIARASDVEVGAFTYDGD
                     TPQDARRAIRGRANVVLTNPDMLHAGVLPHHPKWAKLFGGLRYVVIDELHAYRGVFGS
                     HLANVLRRLRRVCEHYGSSPTFICSSATIANPVELAESLAERPFELVAESGAPRGEKW
                     LVFVNPPIVNRQLGIRRSYISQSRGLALEFLQKGLQVIVFAQSRLSTEILTRYLKDAH
                     DGAPGESDLIRGYRGGYLPHRRREIERGLREGAVRGVVSTNALELGIDIGALDVAVLA
                     GYPGTIASTWQRFGRARRRSGRSAGVLVASSAPLDQYVVRHPAYFFDASPEHALVNPD
                     NLQILVDHVKCAAFELPFATTDTFGRENVQDILGILAEEGFVHLSRPGSAEGAAGRWH
                     WTSESYPADAISLRTVSSDNFVVVDVTGKPRDIGETDFNTALEILHEKAIYIVEGRLY
                     QVERLDVEGRKAYVRHVDCDYYTDAITYSRVTP"
     gene            1969..2739
                     /locus_tag="PLT35_05375"
     CDS             1969..2739
                     /locus_tag="PLT35_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG28692.1"
                     /translation="MDVGLFRKCRYTAAKARDAIVDDTQASRRPPEPARILVVDDDAE
                     LCELMKPFFERHGFALDAEHDGVRGLVAALEGRHDLIILDVMLPGIDGIQLLRRLRRS
                     SNVPVILLTARTDMDDRIQGFDAGADDYLPKPFGPEELVARIRAVLRRAGRAGEVSRA
                     AFHAGGLDVRPASRDVLVDGALLPLTSIEFDILECLARAAGRVVSRDVLAALLHQRDA
                     AAFDRSVDVHVHHLRKKLGRRRDLIRTIRGEGYLFRAD"
     gene            2758..4041
                     /locus_tag="PLT35_05380"
     CDS             2758..4041
                     /locus_tag="PLT35_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG28693.1"
                     /translation="MRSILGKILCWLAGTLVLGAVSFVVVSVLLIERRARDPLAAALL
                     LQLEGATRTYEARGAPALEAYLERLHRDFGGRFHLADAAGRDVLTGEDRSPAIAGALP
                     RPALWPPRRSAPVVAATADGRYHFIAFSPHPIDVVEVSPYYVWIPLAIVALAYLLAKH
                     LAAPARRLRDTVERFGRGDLSVRCHSASRDEFGDLARAFDRMADRIEGTLENERRLLR
                     DVAHEIRSPLARLKYAVELAGTDQGRDTALAHIRKDIDRMASLASDLLETTRPVFGPA
                     ASGRDVPLGALVAEVAADCRRDAESESRPISVDVPPGLRARADPERVRRAVENLVRNG
                     LLHTPPGTPVEIRAVQAGDVVRLTVRDWGPGVPPAELERIFRPFHRLDEARRRETGGT
                     GLGLAIAERAIAGAGGRIAARNMNPGLEIAIDLPC"
     gene            4203..6671
                     /gene="bamA"
                     /locus_tag="PLT35_05385"
     CDS             4203..6671
                     /gene="bamA"
                     /locus_tag="PLT35_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOG28694.1"
                     /translation="MTCLAWLVIAFTGQQVPAPTALPPPDSPPLVRTVEIAFPTQGNV
                     SAIDPATYLFYIKTMPSRPSAGVWSPYDSASVLEDFKRLWGTGFLDNIWIEVKDVPYP
                     NGVEGKHIVFNLEERQRVKIVDYAGSKAIEQSKIEEKLREEGLQLRVDSFIDPGTIRR
                     VEGLVRGMLAEKGYEYGEVTHAIRPMESDPKLVTLTFTINEGPKVRIRHVEFIGNRAV
                     SDRKLRRRLKFNKAEWGLSWLTGRGTYQATKLEEDADRLTEYYRNNGYVTARIGQPTL
                     RTIGDSKDGRTRYVALEVPVQEGERYRIGTVAFDGNTVLKPEALTQLFPLKKGRYYDE
                     SRIRKALDKAREAYGSAGYYEFTGYPDLTPREPMSPDEAALSPTPPAPPTVDITMRLQ
                     EGKQYFVRRIEFTGNTTTRDRVIRRELAFYEGGVFNVEALKYTVKRLNQLGYFKPVED
                     QKNIKVDKATDADNQVDVTLKFEEQNRNQLNFGAGMSQYDGFFGTFAYTAANFLGRGE
                     GVTLALQRGSRSSLYQFSINEPYLFDQPISASFDLYSRKNNYYTAINVVGYSEVREGA
                     SLSIGRPLRRFMHGYLGYSYEVIQIAISRDLLKATSGSSLYGMPLFSFGLDSGRHTDS
                     RIMPALSYNTVDQPLFPRSGLRLTLSGSLASEQLGGSYNYAKPEGEAVFYLPISRRTS
                     LGVRAQGGWLRRYGTTRNVPYYLRYFLGGETQIRGVDIRSVGPVDQYHRSLGGNKFML
                     FNAEYSFDVMSQVRFLLFHDAGQAFAEGERLDVRKLRTSTGAELRVMVPMLNVPFRLI
                     YAVNLYKDIFQPRSAFKFAVGTTF"
BASE COUNT         1059 a         2294 c         2387 g         1038 t
ORIGIN      
        1 gcggcgtgac gcggctgtag gtgatggcgt cggtgtagta gtcgcagtcc acgtggcgga
       61 cgtaggcctt gcgtccctcg acgtcgagcc gctcgacctg atagagccgc ccttcgacga
      121 tgtagatggc cttctcgtgc aggatctcga gggcggtatt gaagtccgtc tcgccgatgt
      181 cgcgcggctt cccggtcacg tccacgacga cgaagttgtc cgacgacacc gtgcgcaggc
      241 tgatcgcgtc ggcgggatac gactcgctgg tccagtgcca gcgcccggcg gcgccctcgg
      301 ctgaaccggg tcggctgagg tggacgaagc cctcctcggc caggatgccg aggatgtcct
      361 gcacgttctc gcgcccgaac gtgtcggtcg tcgcgaaggg cagctcgaag gcggcgcact
      421 tgacgtgatc gacgaggatc tggaggttgt cggggttgac cagcgcgtgc tccggagacg
      481 cgtcgaagaa gtacgccggg tgccggacga cgtactgatc cagcggcgcg ctagacgcga
      541 ccagcacgcc ggcggaacgg ccagagcggc ggcgcgcgcg gccgaaccgc tgccacgtgg
      601 acgcgatggt cccggggtag cccgcgagca ccgcgacgtc cagcgccccg atgtcgatcc
      661 cgagctcgag cgcgttcgtg gacaccacgc cgcgcacggc accctcgcgc agcccgcgct
      721 cgatctcgcg ccgccggtgc ggcaggtagc cgccccggta gccgcggatg agatcggact
      781 cgcctggcgc gccgtcgtgc gcgtccttca ggtagcgcgt gagaatctcc gtggacagcc
      841 ggctctgcgc gaagacgatg acctgcaggc ccttctgcag gaactccagc gccaggccgc
      901 gcgattgcga gatgtacgag cgccggatcc ccagctgccg gttcacgatc ggcggattga
      961 cgaagacgag ccacttctcc ccgcgcgggg cgccgctctc ggccaccagc tcgaagggcc
     1021 gctcggcgag gctctcggcc agctccacgg gattcgcgat cgtcgccgac gagcagatga
     1081 aggtcggcga cgagccgtag tgctcgcaca cgcggcgcag ccgccggagc acgttggcca
     1141 ggtggctgcc gaacacgccg cggtaggcgt ggagctcgtc gatgacgacg tagcgaagcc
     1201 ccccgaagag cttggcccac ttcgggtggt gcgggagcac gcccgcgtgc agcatgtccg
     1261 ggttcgtcag gaccacgttc gcgcggcccc ggatcgcccg cctggcgtcc tgcggggtat
     1321 cgccgtcgta cgtgaaggcg ccgacctcca cgtccgacgc cctggcgatc tcccgggtca
     1381 gccgatcgag ctcggccagc tgatcctggg ccagcgcctt cgtcgggaac aggtagagcg
     1441 cccgcgagga cgggtccgcc aggacggcgt tcatcacggg gatgttgtag cagagcgttt
     1501 tccccgaggc ggtcggcgtc gtcaccacga cgtggcggcc ggcgagggca tgcgccaccg
     1561 cccgcgcctg atggctgtag agccggtcga tcccccggcg ggccagcgcg tcgcgcaggc
     1621 gcggatcgac gagcggcggg aggtcctcga agtcggcgtc gcgtgcggga acacgccgga
     1681 cggcagtgac gaggccgtcg ggctggtcgg ggcgctccct gacagcctgg tccggcagca
     1741 cctcgcgcaa cgccatctcg agcgcctggt cccggcgcgc gtcgcctccc ggaagcatgg
     1801 gcggggtccg cacagccggc atcgtactag ccgtggcggt accgatcaag aggcgaaagt
     1861 ctcggcgcga cgcgccggag ccgcggccgc tgccgtccag gcccggaccg ggcgcgcccg
     1921 ttcgcgcgtt cggggacgcc gccggcaacg cgcctggccg cgcggcccgt ggacgttggc
     1981 cttttccgca agtgccggta cactgcagcg aaggctcgcg acgcgatcgt ggacgacacc
     2041 caggcaagcc ggcgcccgcc ggaaccggcg cgcatcctcg tcgtggacga cgatgccgag
     2101 ctgtgcgagc tgatgaagcc gttcttcgag cgccacggct tcgcgctgga tgccgagcac
     2161 gacggcgtgc gcgggctggt cgccgccctc gagggccggc atgacttgat catcctcgac
     2221 gtcatgctgc cgggcattga cggaatccag ctcctgcgcc gcctccggcg gagcagcaac
     2281 gtccccgtca tcctgctcac cgcgcgcacg gacatggacg atcggatcca ggggttcgac
     2341 gccggcgccg atgactatct gccgaagccg ttcgggcccg aggaactggt ggcccggatc
     2401 cgggcggtgc tgcggcgggc gggccgggcc ggggaggtct cgcgcgcggc gtttcacgcc
     2461 ggcggcctgg acgtcaggcc ggcgtcgcgt gacgtgctgg tcgacggcgc gttgctgccc
     2521 ctgacctcga tcgaattcga catcctcgag tgcctcgcgc gcgccgccgg ccgcgttgtc
     2581 tcgcgcgacg tcctggcggc gctgctgcac cagcgcgacg cggcggcgtt tgaccggtcc
     2641 gtggacgtcc acgtccacca tctgaggaag aagctcggcc gtcggcggga cctcattcgc
     2701 acaatcaggg gcgagggcta cctgttccgc gcggactagg cgaggcggag agcgccgatg
     2761 cggtccatcc tcggcaagat tctctgctgg ctggccggca cgctcgtgct cggcgcggtc
     2821 tcgttcgtcg tggtatcggt cctcctcatc gagcgccggg cccgcgatcc gctggcggcg
     2881 gcgctgctgc tccagctcga gggggccacg cgcacgtacg aggcccgcgg cgccccggca
     2941 ctcgaggcct acctggagcg gctgcatcgg gacttcggcg gacgcttcca tcttgcggac
     3001 gccgccggcc gcgacgtgtt gacgggcgag gaccggtcgc cggccatcgc tggcgcgctg
     3061 cctcgtccgg ccctgtggcc tccgcgtcga agcgcgcccg tcgtggcggc gacggccgac
     3121 gggcgctacc acttcattgc gttctctccc catcccatcg atgtggtgga ggtgtcgccc
     3181 tactacgtgt ggatcccgct ggcgatcgtg gcgctcgcct acctcctggc gaagcatctg
     3241 gctgcgccgg cgcgccgtct gcgcgacacc gtggagcgct tcggccgggg ggacttgtcg
     3301 gtgcgctgcc attccgccag ccgcgacgaa ttcggcgatc tggcgcgcgc cttcgaccgg
     3361 atggccgacc gcatcgaagg aacgctggag aacgagcggc gcctcctgcg ggacgtggct
     3421 cacgaaatcc gctccccgct cgcgcggctc aagtacgccg tcgagctggc ggggaccgat
     3481 cagggccgcg acaccgcgct ggcgcacatc cggaaggaca tcgatcggat ggcgtcgctc
     3541 gcgtcggatc tgctcgagac gactcgtccc gtgttcgggc cggcggcgag cggccgcgac
     3601 gtgccactgg gcgcgctggt ggccgaggtc gccgccgact gccggcggga cgcggagtcg
     3661 gagagccggc cgatcagcgt ggacgtgcct cccgggctcc gcgcgcgggc cgatccagag
     3721 cgggtcaggc gggccgtcga gaacctcgtc cggaacggcc tcctgcacac tccgcccggg
     3781 acgccggtgg agatccgggc cgtgcaggcg ggagacgtgg tgcgcctcac cgtccgcgac
     3841 tggggcccgg gcgtgccgcc ggccgagctc gagcgcatct tccgcccgtt tcaccgcctg
     3901 gacgaagcgc gccggaggga gacggggggg acgggtctgg ggctggcgat tgcggagcgg
     3961 gcgattgcag gggcgggggg ccggattgcc gcccgtaaca tgaatcccgg cttggagatc
     4021 gccatcgatc tcccgtgcta acaaaactta acaaatgcgt tccgaacaat ccgacagaat
     4081 gggagtctga accacgagag ggtgcggcgg gaacctgtct ccctgcctga tatcatagac
     4141 ggtcacggac cgtccgttgt ctcgggtagc gcctgcctga agcgcgacgg agcgcccaat
     4201 ctatgacgtg cctggcatgg ctggtgattg ccttcaccgg tcagcaagtc cctgcgccaa
     4261 ccgcgttgcc gcctccggac tcgcctccgc tggtccggac ggtcgagatc gcgttcccga
     4321 cccagggtaa cgtctcggcc atcgatccgg cgacttacct cttctacatc aagacgatgc
     4381 cgagccggcc gtcggccggg gtgtggtctc cctacgacag cgcgtcggtg ctcgaggact
     4441 tcaagcgtct ctggggcacg gggttcctcg acaacatctg gatcgaagtc aaggacgtgc
     4501 cctacccgaa cggggtcgag ggcaagcaca tcgtcttcaa cctcgaagaa cggcagcgcg
     4561 tgaagatcgt cgactacgcg ggctcgaaag cgatcgagca gtcgaagatc gaggagaagc
     4621 tccgcgagga gggcctccag ctccgggtcg attcgttcat cgaccctggc accatccggc
     4681 gggtcgaggg gctcgtccgg gggatgctgg ccgagaaggg gtacgagtac ggcgaggtga
     4741 cgcatgcgat ccggccgatg gagagcgatc ccaagctcgt gaccctgacc ttcaccatca
     4801 acgaggggcc caaggtccgc atccggcacg tggagttcat cggcaacagg gcggtgagcg
     4861 accggaagct ccggaggcgc ctgaagttca acaaggcgga gtggggcctg tcgtggctca
     4921 cgggccgggg aacgtaccag gccacgaagc tcgaggaaga tgcggatcgg cttaccgagt
     4981 actaccgcaa caacgggtac gtgaccgctc ggatcggcca gccgacgctg cggacgatcg
     5041 gggactcgaa ggacggcagg acgcggtacg tggcgctgga ggttccggtt caggagggcg
     5101 agcgctaccg gatcggcacg gtggccttcg acggcaacac cgtgctgaag ccggaagccc
     5161 tgacgcagct ctttccgctg aagaagggcc ggtactacga cgagagccgc atccgcaagg
     5221 cgctcgacaa ggccagggag gcgtacggct cggccgggta ctacgagttc acggggtacc
     5281 ccgatctgac gccgcgcgag ccgatgtcgc ccgacgaggc ggcgctgtcg ccgacgccgc
     5341 ccgcccctcc gaccgtggac atcacgatgc gcctgcagga gggcaagcag tacttcgtcc
     5401 gccgcatcga gttcacgggg aacacgacga cgcgggaccg ggtgatccgg cgcgaactgg
     5461 ccttctacga aggcggcgtg ttcaacgtcg aggccttgaa gtacacggtc aaacggctca
     5521 accagctcgg gtacttcaag ccggtcgagg atcagaagaa catcaaggtc gacaaggcga
     5581 ccgacgcgga caaccaggtg gacgtcacgc tgaagttcga ggagcagaac cgcaaccagc
     5641 tcaacttcgg cgcgggcatg tcgcagtacg acggcttctt cggcaccttc gcgtacacgg
     5701 ccgcgaactt cctgggccgc ggcgagggcg tgacgctggc gctccagcgc gggtcgcggt
     5761 cgagcctcta ccagttcagc atcaacgagc cgtacctctt cgaccagccc atcagcgcgt
     5821 cgttcgacct ctactcgcgg aagaacaact actacacggc catcaacgtg gtcggctaca
     5881 gcgaggtgcg cgagggcgcg tcgctgtcga tcgggcggcc gctccggcgg ttcatgcacg
     5941 gctacctggg ctacagctac gaggtcattc agattgcgat cagccgcgac ctcttgaagg
     6001 cgacgagcgg atcgagcctc tacggcatgc cgctgttcag cttcggtctc gactcggggc
     6061 ggcacaccga cagccggatc atgccggcgc tctcgtacaa cacggtggac cagcccctct
     6121 tcccgaggtc ggggctgcgc ctcacgctga gcgggtccct cgccagcgag cagctgggcg
     6181 gaagctacaa ctacgcgaag cccgagggcg aggcggtctt ctacctgccc atctcccgga
     6241 ggacctcgct cggggtgcgg gcccagggcg gctggctgcg ccgctacggc acgactcgga
     6301 acgtgccgta ctacctgcgc tacttcctcg gcggggagac ccagatccgc ggcgtggaca
     6361 tccgctcggt cggcccggtc gaccagtacc accgctccct cggcggcaac aagttcatgc
     6421 tgttcaacgc cgagtactcg ttcgacgtga tgagccaggt ccgcttcctc ctcttccacg
     6481 acgccggcca ggcgttcgcc gaaggagagc gcctggacgt ccgcaagctg cgcacgtcca
     6541 ccggcgcgga gctgcgcgtc atggtcccga tgctcaacgt gccgttccgg ctcatctacg
     6601 cggtgaacct ctacaaggac atcttccagc cgcgctccgc cttcaagttc gccgtgggca
     6661 cgacgttcta atcctcaagg agtaagtcag atgcggaagt tcgcgtatct cctgtggctg
     6721 ccgttcctcg tcggggccgt cctcgggcag gacgtagggc gttccgtcgt aacgaagt
//
LOCUS       DAOSIF010000115         6769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10217_length_6769_cov_3.379506, whole genome shotgun sequence.
ACCESSION   DAOSIF010000115 DAOSIF010000000
VERSION     DAOSIF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10217_length_6769_cov_3.379506"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLT35_05390"
     CDS             <1..452
                     /locus_tag="PLT35_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28695.1"
                     /translation="IRVQRQMFAILREEHDPARIAERLNALDVPGGPAAKAQVVRQGR
                     SPWLRFFVRYDPAAALAKVPCPVLGLWGELDTQVEPAQNYGPVEAALQRGGHRDYTLQ
                     RLPGLNHLLQPAKTGLPSEYARIEETMSPGALETIGAWVRARTGLAK"
     gene            complement(498..1187)
                     /locus_tag="PLT35_05395"
     CDS             complement(498..1187)
                     /locus_tag="PLT35_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28696.1"
                     /translation="MRQVLEQAWRQFAAQSLSMLPNVLASLLFLVIGVALAVAAGRVA
                     RFLLRRSAVERRASRLGVTSWLERAGILSATAALVRLVQIVFALMTAALVLYALDAEL
                     AADLTRRFFLYLPDLAVGIGIFVVGLLAARVAGRSVLIGAVNHGIGAARLLATGTRGA
                     VIVLTGAMALDQLGIGKSIVPSALIILLGGVTLAAALAVGFGSRDTVGRWLDDRLRRP
                     APADADDIQHW"
     gene            complement(1225..2535)
                     /locus_tag="PLT35_05400"
     CDS             complement(1225..2535)
                     /locus_tag="PLT35_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall biosynthesis glycosyltransferase"
                     /protein_id="HOG28697.1"
                     /translation="MSAPDILAPAVRGRLDQIGRADIVVGIPSYQSAATIGHVVQAAQ
                     TGLAKYFPSAQSVILNADGGSTDGTPAVVESTSGPDTVLVMVSQPERPIQRFTIPYHG
                     LPGKGSAFRTIFAAAEALGATACAVVDSDLRSITPEWIHLLLAPILDRTHDYVAPYYL
                     RHKFDGTITNNVVYPLTRALYGKRIRQPIGGEFGMSLPLIRHYLRQSVWETDVARFGI
                     DIWMTTEAVCGDFRVCQAFLGAKIHNAKDPGADLSAMLVQVLGTVFRLMEAHEARWQP
                     VRATEAVPVFGFQYGVGLEPVHVDVGRMIDHFRRGVRDLAEIWGSMFGRDDMARLRTA
                     AASGGDSLAIGDDLWVRLIYDLAAAYRRRASDRDALVRATLPLYMGRVASFVLEMAGA
                     DAAEVEARLERLCSAFEAQKDYLRRRWDAAHGPPSRRGRTRRIA"
     gene            complement(2532..3398)
                     /locus_tag="PLT35_05405"
     CDS             complement(2532..3398)
                     /locus_tag="PLT35_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOG28698.1"
                     /translation="MRLVIVTDLDGTLLDAATRALGPAEAVVGRVVRAGVPLVLSSSK
                     TRAEIEALQRQLGLAQPFIAENGGAIVAPPGSFADGRPAPAGADGRFVIELGRPYREV
                     VAIVREAAEAEGVRVVGFSDMTVGDLAADSGLSPLEAQLAKLREYDEPFRLIDADTAA
                     RRRFLKALRRRGLQAVAGGRYDHAMGDTDKGRAVEVLRGLYRDRIGPVVLAGLGDAMN
                     DLPMLRAVDHPVIVRGTSDDSTVRLLRQVPDARVTQARGPAGWAEAVTAMLDAWEGNA
                     VRVPPRFWPGLA"
     gene            complement(3417..4640)
                     /locus_tag="PLT35_05410"
     CDS             complement(3417..4640)
                     /locus_tag="PLT35_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28699.1"
                     /translation="MPDFYQTGVVTTLHRLNRTKVEHLERDLERFTVAQPVALVLPCL
                     IDELDRPALGHIVEQLRGVRYLDTVVVSLGRATEDGVGRARDLFAKSRQRVRILWLDG
                     PRVVAALDRLRECGLLPSTSGKGFACWLAYGLVLAEARCHAIAVHDCDITTYDRELLA
                     RLVYPVAHPSLGFEFAKGYYARVGKKMNGRVTRLFMTPLLRSLQVVVGRHPLLVYLDS
                     FRYALAGEFAMKTELARVNRIPSDWGLEIGVLAEVHRNCALKRICQTELCANYDHKHQ
                     SVSADDASKGLLRMCTDIAQNLVRTLAAEGITFTQGHFQTLLVQYIRMAQDTITRYHA
                     DAAINGLAFDRHDEESMVGVFSGALRTACERFLDDPLGAPLIPNWNRIVAAIPDFLDE
                     LRTAVDEDGIEHAVG"
     gene            4769..6172
                     /locus_tag="PLT35_05415"
     CDS             4769..6172
                     /locus_tag="PLT35_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG28700.1"
                     /translation="MRDAADLRPGPPPADALTGAGGLPLGAMLVAGFALTILLWLAAG
                     IDLTFTFRAIDREVGAMTDRFMESEQALADVRTNVLLGAIDWRDSLLDSGEPDRTEAY
                     LEQLRGFQAACAASLQKLRASSDGLAASVSLEDLAREVDEYWASVVPIVRLPQARRAS
                     DVRRILGERILPRRANVVRIVAHVQSLNRSRLAEQQRREAAIYSRSSARFLLTGGVTL
                     LLSLAVGAFVTARVSRLERGLRTQLAANAAIAADLHRLSARLVRAQEDERRRIARELH
                     DEVGQALTAVKLQLSVARRAEPGSAGAALDEARAVADSALQSVRQLSRLLHPPMLDDM
                     GLAPALDWYLQAFADRAGVAMEFVHAGMADRPAPEVETCLFRVVQEATTNIAKHAAAT
                     ACRVYLQRLPASVVLTVEDNGRGFDPGTARQRAPEGFGLMSIEERVADARGVFRLESA
                     PGRGTRLTVELPAGGAS"
     gene            6169..>6769
                     /locus_tag="PLT35_05420"
     CDS             6169..>6769
                     /locus_tag="PLT35_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG28701.1"
                     /translation="MIRLLLADDHTLVRQGLRRILEERPDWRVVAECGDGREAVRRAV
                     ELAPDVAVLDIGMPALGGIEAARQIARQCPGTRILILSMHADEAHITQAMEAGASGYL
                     LKDSADDDLVRAVQATVQGKSFFSPAAAAVLLDEYRRSLARRGVTDRYDSLSGREREV
                     FQLMAEGHSNKSIAAILGVSPATVETHRAHVLEKLDLHSV"
BASE COUNT          952 a         2444 c         2470 g          903 t
ORIGIN      
        1 acatccgggt gcagcggcag atgttcgcca tcttgcgcga agagcacgac cccgcgcgga
       61 tcgccgagcg gttgaacgcc ctcgacgtgc ccggcggacc tgcggccaag gcccaggtgg
      121 tcaggcaggg gcggtcgccg tggctgcgct tcttcgtgcg ctacgacccc gcggccgcac
      181 tggcgaaggt cccctgcccc gtgctggggc tctggggcga actcgatacc caggtggagc
      241 cggcccagaa ctacggtccg gtcgaagcgg cgctgcagcg cggaggccac agggactaca
      301 cgctccagcg cctccctggc ctgaaccacc tgctgcagcc ggcgaagacc ggcctgccct
      361 ccgaatacgc gcgcatcgag gagacgatgt cacccggagc cctcgagacg atcggggcgt
      421 gggtgcgggc gcgcacgggg ctggcgaagt agccccgccc ggcggcgcgt cggccccggc
      481 cggcgcgcct gtccggctca ccagtgctgg atgtcgtccg cgtcggccgg cgcgggccgg
      541 cgcagccggt cgtcgagcca gcgcccgacg gtgtcgcggc ttccgaagcc gacggcgagc
      601 gccgcggcga gcgtcacgcc tccgagcagg atgatgagcg ccgagggcac gatggacttc
      661 ccgatcccga gctggtcgag ggccatcgcg ccggtgagca cgatgacggc gcccctggtg
      721 ccggtcgcca ggagccgcgc cgcgccgatg ccgtggttca cggcgccgat gagcacgctc
      781 cggccggcga cgcgcgcggc cagcagcccg accacgaaga tcccgatccc caccgccagg
      841 tcgggcaggt agagaaagaa ccgccgcgtc aggtcggccg ccagttcggc gtcgagggcg
      901 tagagcacca gcgccgcggt catcagcgcg aacacgatct gcaccagccg caccagcgcg
      961 gccgtcgcgc tcaggatccc cgcccgttcc agccacgagg tcacgccgag ccgggacgcc
     1021 cggcgttcga ccgccgagcg gcgcagcagg aaccgcgcca cgcggccggc ggccacggcc
     1081 agcgcgacgc cgatcaccag gaacagcagg ctcgccagca cgttcgggag catcgacagg
     1141 gactgggccg cgaactgccg ccacgcctgc tcgagcacct gtctcacgac ttgccacatt
     1201 ccgacctccc cttctcccgc gccgtcacgc gattcgtctc gtccggccgc gcctcgaagg
     1261 cggcccgtgc gcggcgtccc accgccgcct caggtagtct ttctgggcct cgaacgccga
     1321 gcacaggcgc tcgagccgag cctcgacctc ggctgcgtcg gccccggcca tctcgaggac
     1381 gaacgacgcc acgcgcccca tgtagagcgg cagggtcgct cggacgagcg cgtcgcggtc
     1441 gctcgcgcgg cggcggtacg cggcggcgag gtcgtagatc agccggaccc acaagtcgtc
     1501 gccgatcgcc aggctgtcgc cgccggaggc cgccgccgtg cgcaggcgcg ccatgtcgtc
     1561 gcggccgaac atcgagcccc agatctcggc caggtcgcgc acgccgcggc ggaaatgatc
     1621 gatcatgcgg ccgacgtcca cgtggaccgg ctccaggccc acgccgtact ggaacccgaa
     1681 gaccggcacg gcctccgtgg ccctgacggg ctgccagcgc gcctcgtgcg cctccatcag
     1741 ccggaacacc gtgcccagca cctggaccag catcgcgctc aggtccgcgc cggggtcctt
     1801 cgcgttgtgg atcttcgccc cgaggaacgc ctggcagacc cggaagtccc cgcacacggc
     1861 ctcggtcgtc atccagatgt cgatgccgaa gcgcgccacg tccgtctccc acaccgactg
     1921 gcgcaggtag tgccggatga gcggcagcga catcccgaac tcgccgccga tcggttgccg
     1981 gatccgcttg ccgtacagcg cccgggtgag ggggtagacg acgttgttcg tgatggtgcc
     2041 gtcgaacttg tgccgcaggt agtacggcgc cacgtagtcg tgggtcctgt ccaggatcgg
     2101 cgcaagcagc aggtggatcc attcgggggt gatgctgcgc agatccgagt ccacgacggc
     2161 gcacgccgtc gcgccgagcg cctcggccgc cgcgaagatg gtccggaagg cgctgccctt
     2221 ccccggcagg ccgtggtagg ggatggtgaa ccgctggatc ggccgctccg gctgcgacac
     2281 catgacgagc acggtgtccg gcccgctcgt gctctcgacg acggctggcg tgccgtcggt
     2341 cgatccgccg tccgcgttga ggatgaccga ttgcgccgac gggaagtact tcgccaggcc
     2401 cgtctgcgcc gcctgcacga cgtggccgat cgtcgcggcg ctctgatagc tcgggatccc
     2461 gacgacgatg tcggcccggc cgatctggtc gagacggccg cgcaccgccg gggccaggat
     2521 gtcgggcgcg ctcatgccag tcccggccag aaccgcggcg gcacgcggac ggcgttcccc
     2581 tcccaggcgt cgagcatggc ggtgaccgcc tcggcccatc ccgctgggcc gcgcgcttgg
     2641 gtcacgcgcg cgtcgggcac ctggcggagc aggcgcacgg tcgagtcgtc ggaggtgccg
     2701 cggacgatga cgggatgatc gacggcccgc agcatcggca gatcgttcat cgcgtcaccc
     2761 agccctgcca gcaccaccgg gccgatgcgg tcccggtaga ggccgcgcag cacctccacc
     2821 gcccgcccct tgtcggtgtc tcccatcgcg tggtcgtagc gcccgccggc gacggcctgc
     2881 aggccgcgcc gccggagcgc cttgaggaag cggcggcgcg cggccgtgtc cgcgtcgatc
     2941 agccggaacg gctcgtcgta ctcgcggagc ttggcgagct gggcctcgag cggcgacagg
     3001 ccggagtcgg cggcgaggtc gcccacagtc atgtccgaga agcccacgac gcggacgccc
     3061 tcggcctccg cggcctcgcg gacgatggcc acgacctcgc ggtagggccg ccccagctcg
     3121 atcacgaagc gcccgtcggc gccggccggc gccgggcgcc cgtcggcgaa ggagccgggg
     3181 ggagccacga tcgccccgcc gttctccgcg atgaacggct gggccaggcc cagctggcgc
     3241 tgcagcgcct cgatctcggc ccgcgtcttg ctcgacgaca gcaccagcgg cacgcctgcg
     3301 cgcaccaccc ggccgacgac ggcctccgcc ggcccgaggg cgcgggtggc cgcgtccagc
     3361 agggtgccgt cgaggtccgt gacgatgacg aggcgcatgg ccgctcgttg ctccccctat
     3421 cccaccgcgt gttcgatgcc gtcttcgtcc accgccgtgc gcagctcgtc caggaaatcc
     3481 gggatcgccg cgacgatccg gttccagttc gggatgagcg gggccccgag gggatcgtcc
     3541 aggaagcgtt cgcacgcggt tcgaagcgcc cccgagaaga cgccgaccat cgactcctcg
     3601 tcgtggcggt cgaaggccag gccgttgatc gccgcatcgg cgtggtagcg cgtgatcgtg
     3661 tcctgggcca tccggatgta ctggacgagc agcgtctgga agtggccctg ggtgaaggtg
     3721 atgccctccg ctgccagcgt gcggacgagg ttctgcgcga tgtccgtgca catgcgcagc
     3781 aggcccttgg acgcatcgtc ggcggacacg ctctggtgct tgtggtcgta gttggcgcac
     3841 agctccgtct ggcagatccg cttgagggcg caattccggt ggacttcggc cagcacgccg
     3901 atctccagcc cccagtcgga gggaatccgg ttgacgcgcg ccagttccgt cttcatggcg
     3961 aactcccctg ccagcgcgta ccggaagctg tcgaggtaca cgagcagggg atggcgcccg
     4021 acgacgacct ggagcgatcg cagcagcggc gtcatgaaga ggcgggtcac gcggccgttc
     4081 atcttcttgc cgacgcgcgc gtagtagccc ttggcgaact cgaatcccag cgacgggtgc
     4141 gcgacgggat acaccagccg cgcgagcagc tcgcggtcgt acgtcgtgat gtcgcagtcg
     4201 tggacggcaa tcgcgtggca gcgcgcctcg gcgaggacga ggccgtaggc gagccagcag
     4261 gcgaagccct tgcccgacgt cgaaggcagg agcccgcact cgcgcagccg gtcgagggcg
     4321 gccacgacgc ggggcccgtc cagccacagg atccgcacgc gctggcgcga cttggcgaac
     4381 agatcgcggg ccctgccgac gccgtcctcc gtggcgcgcc ccaggctgac gaccaccgtg
     4441 tccagatacc ggaccccccg cagctgctcc acgatgtgcc cgagcgccgg ccggtcgagc
     4501 tcgtcgatga gacacggcag cacaagggcc accggctgcg cgacggtgaa ccgctccagg
     4561 tcgcgctcga ggtgctccac cttcgtgcgg ttgaggcggt ggagcgtcgt cacgacgccg
     4621 gtctgataga aatccggcat gctggctgcg ccctcccttg gccatcgtag aagagccgga
     4681 cggcgagggc catccggtcg cgaccggatt cagagcggaa cgagacggga ggcgcgccgc
     4741 cccgggcgca acgtcgtaca atcacggcat gcgggacgcc gcggatcttc ggccgggccc
     4801 gcccccggct gacgccctga cgggcgccgg gggcctgccc ctgggcgcga tgctggtggc
     4861 cggcttcgcg ctgacgatcc tgctgtggct ggcggcgggc atcgacctga cattcacctt
     4921 ccgggccatc gaccgcgaag tgggcgcgat gacggaccgg ttcatggagt cggagcaggc
     4981 gctggccgac gtgcggacca acgtcctgct cggggcgatc gactggcgcg actcgctgct
     5041 cgactcgggc gagcccgatc gcaccgaggc ctacctggaa cagctgcgcg gctttcaggc
     5101 ggcctgcgcc gccagcctgc agaagctgcg cgccagcagc gacggcctcg ccgcgtccgt
     5161 gtcgctggag gacctggcgc gggaggtgga cgagtactgg gcgtcggtcg tgccgattgt
     5221 ccggctgccc caggcgcggc gggcctcgga cgttcgccgg atcctcggcg aacgcatcct
     5281 cccccgccgg gcgaacgtcg tccgcattgt cgcgcacgtg cagtcgctga accggtcgcg
     5341 gctcgcggag cagcagcgcc gcgaggccgc catctacagc cggtcgtcgg cgcggttcct
     5401 gctgacgggc ggcgtgacgc tgctgctgag cctggctgtc ggggcgttcg tcacggcccg
     5461 ggtgagccgg ctcgagcggg gcctccgaac gcagctcgcc gccaacgccg cgatcgccgc
     5521 cgacctgcac cgcctgtcgg cgaggctggt gcgcgcgcag gaagacgagc gccggcggat
     5581 cgcgcgggag ctgcacgacg aggtcggcca ggccctgacg gccgtgaagc tgcagctgtc
     5641 ggtggccagg cgcgccgagc cggggtcggc cggcgcagcc ctcgacgagg cccgcgccgt
     5701 ggccgactcg gcgctccagt ccgtgcggca gctgtcccgg ctgctgcacc cgccgatgct
     5761 ggacgacatg gggctggcgc ccgcgctcga ctggtacctc caggccttcg cggaccgcgc
     5821 cggcgtggcg atggaattcg tccacgcggg catggccgat cggccggcgc ccgaggtgga
     5881 gacctgcctg ttccgcgtgg tgcaggaggc cactacgaac atcgcgaagc acgccgcggc
     5941 gaccgcgtgc cgcgtgtacc tccagcgcct gcccgcgagc gtcgtgctga cggtggagga
     6001 caacgggcgg ggattcgatc ccggcacggc gcggcagcgc gcgcccgagg ggttcggcct
     6061 gatgagcatc gaggagcggg tggcggatgc gcgcggcgtg ttccgcctcg agagcgcccc
     6121 gggccgcggc acgcgcctga ccgtggaact gccggccggg ggagcctcat gattcgcctc
     6181 ctgctcgcgg acgatcacac gctggtgcgc caggggctcc ggcgcattct cgaggagcgc
     6241 cccgactggc gcgtcgtcgc cgagtgcggc gacggccgcg aggcggtgcg gcgggccgtc
     6301 gagctcgcgc cggacgtggc cgtgctcgac atcggcatgc cggcgctcgg cggcatcgag
     6361 gcggcgcggc agatcgcgcg ccagtgcccc gggacgcgga tcctgatcct gtcgatgcac
     6421 gccgacgagg cccacattac gcaggcgatg gaggccggcg cgagcggcta tctgctcaag
     6481 gactcggccg acgacgatct ggtgcgggcc gtgcaggcga cggtgcaggg caagtcgttc
     6541 ttcagcccgg cggcggccgc ggtcctgctg gacgagtacc ggcggtcgct ggcgcggcgc
     6601 ggcgtcacgg accggtacga ctcgctctcc gggcgcgagc gcgaggtgtt ccagctcatg
     6661 gcggaggggc acagcaacaa gtcgatcgcg gccatcctgg gcgtgtcgcc cgccaccgtc
     6721 gagacgcacc gcgcgcacgt gctcgagaag ctggacctgc actcggtgg
//
LOCUS       DAOSIF010000116         6768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10221_length_6768_cov_4.015194, whole genome shotgun sequence.
ACCESSION   DAOSIF010000116 DAOSIF010000000
VERSION     DAOSIF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10221_length_6768_cov_4.015194"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..935
                     /locus_tag="PLT35_05425"
     CDS             87..935
                     /locus_tag="PLT35_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28702.1"
                     /translation="MASGKGGTGKTTVAVNLARAFGSGVWLADCDVEEPNAHLFLRGT
                     TIGSRTVSLPVPQVEAGLCDACGACARLCQYHAIVSFGTLPLLFPELCHGCGGCVRVC
                     PRGAIAEVDRRIGVVETQRDGHITLVQGRLDVGVALVPPLVRAVKASLPRDMPVIVDA
                     PPGTSCPVIATIRGLDALALVTEPTPFGLHDLSLAVDMVRQLNIPFGVVVNRVGVGDD
                     RVHGFCRREGIPILLDIPDDRRIAEVCSRGRLVVDELPEYRPAFARLWAGACALAAPV
                     PALALS"
     gene            932..1909
                     /locus_tag="PLT35_05430"
     CDS             932..1909
                     /locus_tag="PLT35_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG28703.1"
                     /translation="MTANPTRYVYGPVPSRRLGRSLGVDLVPLKTCSYDCVYCQLGPT
                     SNPTTERREYAPVRDVLSGLQATLASGSAPDYISLAGSGEPTLHSGIGEIIRGIKAMS
                     AVPVAVLTNGSLLWREDVQRDLLQADLVLPSLDAGDEAMFRRVNRPHGGVSFAQVVDG
                     LASFTRQFSGDVWLEVFLLGGLTAEPHEARKIAALVRRIAPTRVQLNTVTRPPADPSA
                     RRVGLKALEGLASLFPGDVDVVCEAPWGHAPAGAAPAAIRDDIMAMLARRPCTAADVA
                     NGLGVPPNEAIKHLDALVSEGVVGPRVVDGRCYYAAVGGQSAAPPSTEA"
     gene            1913..2800
                     /locus_tag="PLT35_05435"
     CDS             1913..2800
                     /locus_tag="PLT35_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28704.1"
                     /translation="MRELVVISGKGGTGKTSLTASLAVLADRPVLADCDVDAADLHLV
                     LEPRQRNRHDFYGGREAVIRQDDCNGCGTCLAVCRFDAVLVDRPPGGRDTYTVDPVAC
                     EGCGVCVHFCPEIAIDFPERLAGEWMVSDTRCGPMVHARLGIASENSGKLVSTVRREA
                     RRIAEDEGRQLVIVDGPPGIGCPVIASVTGASLVLAVTEPTVSGEHDLERVLSLARHF
                     GVPAAVCVNKWDLNPEMTDRIEGRARAAGARVTGRVRYDRGVTAAQIQAKAVVETGAA
                     CGEDIRSIWADLRRHPAIS"
     gene            2928..4343
                     /locus_tag="PLT35_05440"
     CDS             2928..4343
                     /locus_tag="PLT35_05440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG28705.1"
                     /translation="MRRTLSAKRRRTPAPQLAPAPTEAILESISDGVFTVDRDWRITS
                     FNRAAEQITGVSRREAIGQRCEDVFRSSMCGPDCALARTLQSGRPIIGRTAYIIDAEG
                     TRIPISVSTAVLTDAGGRVTGGAETFRDLSELEALRLELEGQHRAGDLSSRSPLMQRV
                     FDALPAMAASPGTILILGETGTGKELVARTIHAFSAHNKGPFVAVNCGALPDTLLESE
                     LFGYRAGAFTGAARDKPGRFALARGGTLFLDEIGEVSPALQVRLLRVLQERTFEPLGA
                     TRSEQTDARIIVATNRDLDDEVRHGRFREDLYYRVNVLRIVLPPLRQRKEDIPLLADE
                     FVARFNRLQQRAVRGVSAEALSVLMAHDWPGNVRELENAIERAFVLCTDGFIGPRHLP
                     EELTARVREPGAPPGIRAAHQELDVQAIRAAIGASGGDRAAAAKALGIHKTTLFRRIR
                     KLGIELPARDGRSRRRKAPSS"
     gene            4538..4936
                     /locus_tag="PLT35_05445"
     CDS             4538..4936
                     /locus_tag="PLT35_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOG28706.1"
                     /translation="MPRPFCHRHVAGQPAAPVFKPAGVPALLLGDVVMTLDEFEALRL
                     ADLERLYHKRAAEQMGVSRPTFSRIVDSARVKIADAIVHGKALRIEGGPVRLLAGPRR
                     CCRLHEGRSRPPTPAGADGHHPRTPKEETT"
     gene            4933..5325
                     /locus_tag="PLT35_05450"
     CDS             4933..5325
                     /locus_tag="PLT35_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG28707.1"
                     /translation="MRICIPVDEDQGLASPVCLHFGSAPYFMIVNTDDGSCRAIPNRN
                     EHHAHGMCQPLQAIQGEAIDGMVVGGIGMGALNKLRAAGICVYQAQHPTVDLAVEAIK
                     AGRLPLVEPGSACGGHHSHGPGGHHHRS"
     gene            5504..6004
                     /locus_tag="PLT35_05455"
     CDS             5504..6004
                     /locus_tag="PLT35_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOG28708.1"
                     /translation="MDKQKSIDLLNKGVADELQAVHQYMYWHFHLDDQGFGPLSSMFK
                     RIAIEEMGHVERLAERILFLKGDVDMVPAGPVERVLEPDAILKRAAEMEHAAVGAYNA
                     FAIQCSQNADAVSKQMFEALVGDEERHFDEFDKQLDNIKRFGPSYLALQSFNTAPESA
                     APPKAD"
     gene            6051..6497
                     /locus_tag="PLT35_05460"
     CDS             6051..6497
                     /locus_tag="PLT35_05460"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HOG28709.1"
                     /translation="MAEPVRVGIIICDRYRTCAGGKCLRALKAREGAFSAYRGREVEL
                     VGFTTCNGCPGGNVEYAPGEMKRNGADVIHLATGLVVGYPPCPRIDAFTTFIAKKYGI
                     PVVIGTHPIPEKYYLTHQACGTWSGPDWPARLAPTLADRAVRLAYD"
     gene            6532..>6768
                     /locus_tag="PLT35_05465"
     CDS             6532..>6768
                     /locus_tag="PLT35_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOG28710.1"
                     /translation="MQAKQPGINSRLVHGTYRPDATGAVNVPIYQSSTFAFRNAEHGA
                     ALFAGREKGYIYTRIGNPTIGAFEDAVASIENGAG"
BASE COUNT         1020 a         2359 c         2424 g          965 t
ORIGIN      
        1 tcggaggcca tcgacgccta ccgggcaggt acgctcacca ggatcgacgc gccggacgtc
       61 gacgggcact ggacatgatg ctcggcgtgg cgtcgggcaa gggcggcacc ggcaagacga
      121 ccgttgcggt caacctggct cgggccttcg gctccggtgt ctggctggcc gactgcgacg
      181 tcgaagagcc gaacgctcac ctcttcctgc ggggcacgac aatcggctcg cgcacggtga
      241 gcctccccgt gccgcaggtg gaggcgggcc tgtgcgacgc gtgcggcgcc tgcgcccgtc
      301 tctgccagta ccacgccatc gtgtcgttcg ggacgctgcc gctcctcttc ccggagctgt
      361 gccacggctg cggagggtgc gtccgcgtct gtccccgcgg cgcgatcgcc gaggtggacc
      421 ggcgcatcgg cgtcgtggaa acgcagcgcg acggccacat cacgctggtg cagggccgcc
      481 tcgacgtcgg cgtggccctc gtgccgccgc tcgtgcgggc cgtgaaggcc agccttcccc
      541 gggacatgcc ggtcatcgtc gatgcgcccc cgggcacgtc gtgccccgtg atcgccacga
      601 tccggggact ggacgcgctg gcgctcgtca ccgagcccac ccccttcggc ctgcacgacc
      661 tctcgctggc cgtggacatg gtccgccaac tcaacatccc gttcggcgtg gtcgtcaacc
      721 gcgtgggcgt cggcgacgat cgcgtgcacg ggttctgccg ccgcgagggc atcccgatcc
      781 tcctcgacat ccccgacgac cgcaggattg ccgaggtctg ctcccgcggg cgcctggtcg
      841 tggacgagct gccggagtac cgccccgcgt tcgcacgcct gtgggccggc gcatgtgcgc
      901 tggcggcgcc ggtaccggcg ctggcgctgt catgaccgcg aacccgactc ggtacgtcta
      961 cggccccgtg ccatcgcggc ggctggggcg atcgctcggc gtcgatctcg tgccgctcaa
     1021 gacgtgctct tacgactgcg tctactgtca gctcgggccg acctcgaacc cgacgacgga
     1081 gcggcgggag tacgcgcccg tccgggacgt cctctcgggc ctgcaggcga cgctggcgtc
     1141 cggaagcgcc ccggactaca tcagcctcgc gggctccggc gaaccaacgc ttcactcggg
     1201 catcggcgag atcatccggg ggatcaaggc catgagcgcc gttccggtcg ccgtcctgac
     1261 caacggctcg ctgctctggc gggaggacgt gcaacgcgac ctgctgcagg ccgatctcgt
     1321 gctgccgtcg ctcgacgcgg gcgacgaggc gatgttccgg cgcgtgaacc gcccccacgg
     1381 cggggtgtcg ttcgcgcagg tggtcgacgg cctcgcgtcc ttcacccggc agttcagcgg
     1441 ggacgtgtgg ctggaggtct tcctgctcgg gggcctgacc gccgagccgc acgaagcgcg
     1501 gaagatcgcg gcgctcgtcc ggcgcatcgc gccgacacgc gtgcagctca acaccgtcac
     1561 ccggcctccg gcggacccgt cggcccgccg cgtgggcctg aaggcgcttg aggggctggc
     1621 atcgctgttt ccgggagatg tcgacgtcgt ctgcgaggcg ccctggggcc atgcgccggc
     1681 cggcgccgcg ccggccgcaa tcagagacga catcatggcg atgctcgcca ggcggccctg
     1741 cacggctgcc gacgtcgcca acggcctggg cgtgccgcct aacgaggcca tcaaacacct
     1801 cgacgccctg gtgtccgagg gcgtcgtcgg tccacgcgtc gtcgacggcc gctgctacta
     1861 cgccgcagtc gggggacagt ctgcggcgcc tccatcaacg gaggcatgac ccttgcgcga
     1921 actggtcgtc atcagcggca agggcgggac cggcaagacg agcctcaccg cgtcgctcgc
     1981 ggtgctggcc gaccggccgg tcctcgccga ctgcgacgtg gacgcggcgg atctccacct
     2041 ggtgctggag ccgcgtcaac ggaaccggca cgacttctac ggaggacgcg aagccgtcat
     2101 ccggcaggac gactgcaacg gatgcggaac ctgcctggcg gtttgccggt ttgatgccgt
     2161 gctcgtcgat cgtccacccg gcggcaggga cacgtacacg gtggacccgg tggcctgcga
     2221 gggttgcggg gtctgcgtcc acttctgccc cgagattgcc atcgacttcc ccgagcggct
     2281 cgccggcgag tggatggtct ccgacacgcg ctgcggcccg atggtgcacg cccgactggg
     2341 gatcgcgtct gagaactcgg gcaaactggt gtcgacggtc cgccgcgagg cccggcgcat
     2401 cgccgaggac gaaggccggc agctcgtcat cgttgacggg ccgcccggca tcgggtgccc
     2461 cgtcatcgcc tcggtgaccg gtgcctcgct ggtgctcgcg gtcacggaac ccacggtgtc
     2521 gggcgagcac gatctcgagc gcgtgctgtc gctcgcgcgg cacttcgggg tgcccgcggc
     2581 ggtctgcgtc aacaagtggg atctcaaccc ggagatgacg gacaggatcg agggccgggc
     2641 tcgggcagcc ggggcgcgtg tgaccggccg ggtgcgctac gatcggggag tgacggcagc
     2701 ccagatccag gcgaaggccg tggtcgaaac cggcgcggcg tgcggcgagg acatccgcag
     2761 catatgggcg gacttgcgcc gccatcccgc gatttcctga tcggggcgcg cggccgggca
     2821 ggcccggcgc atctgtcgcg tgctgacggc cgaagaagcg ggagcgtgcc ggagacaccg
     2881 gcggcgccgt ggagacaggc gctgtcgagc gcgatgcaat catggccgtg agacggactc
     2941 tttcggcgaa gcggcggcgc acgcccgcgc cgcagctcgc gcccgccccg accgaggcca
     3001 tcctcgagag catctcggac ggtgtcttca ccgtggaccg ggactggcgc attacgtcgt
     3061 tcaaccgggc cgcggagcag atcacgggcg tcagccgtcg ggaggccatc ggccagcgct
     3121 gcgaggacgt gttccgctcg agcatgtgcg ggccggattg cgccctcgcc aggacgctgc
     3181 agtccggccg gcccatcatc ggccggacgg cgtacatcat cgacgccgaa gggacgcgga
     3241 tcccgatcag cgtgtccacg gcggtgctga ccgatgccgg ggggcgcgtg actggcggcg
     3301 ccgagacgtt ccgcgacctc tcggagctgg aggcgctccg cctggaactc gagggacagc
     3361 atcgggccgg cgacctgtcg agccggagcc cgctgatgca gcgggtgttc gacgcgctgc
     3421 cggccatggc cgccagcccc ggcacgatcc tgatcctggg cgagacgggc accgggaagg
     3481 agctggtcgc gcggacgatt cacgccttca gcgcccacaa caaggggccg ttcgtggctg
     3541 tcaattgcgg cgccctgccc gacacgctgc tcgagtcgga gctcttcggg taccgggctg
     3601 gcgccttcac cggcgcggcg agggacaagc ccggccgctt cgcgctggca aggggcggaa
     3661 ccctcttcct cgacgagatt ggcgaggtga gcccggcgct ccaggttcgc ctcctgcgcg
     3721 ttctgcagga acgcaccttc gagcctctcg gcgccacccg ctccgagcag accgacgccc
     3781 ggatcatcgt ggcgaccaac agggacctcg acgacgaggt gcggcacggc cgcttccggg
     3841 aggacctcta ctaccgggtc aacgtcttgc gcatcgtcct gccgccgctc cggcagcgaa
     3901 aagaggacat ccccctgctg gccgacgagt tcgtcgcccg gttcaaccgc ctccagcagc
     3961 gtgcggtgcg gggcgtgtcc gccgaggccc tgtccgtgct gatggcccac gactggccgg
     4021 gcaacgtgcg cgagctcgag aacgcgatcg agcgggcctt cgttctctgc acggacggat
     4081 tcatcgggcc gcggcacctt cccgaggagc tgacggcgcg cgtccgggaa ccgggcgcgc
     4141 cgcccggcat cagggcggcc caccaggagc tcgatgtgca ggccatccgc gccgcgatcg
     4201 gcgcgagcgg cggcgaccgc gccgcggcgg cgaaggcact gggcattcac aagacgaccc
     4261 tcttccggcg catcaggaaa ctcggcatcg agttgccggc gcgagatgga cgctcgcgcc
     4321 ggcggaaggc gccctcgtcg tgaccgggcc ggggcaggga gggccggcgg atgcggcccg
     4381 gcccgccgct cgtcgagccg cgccgcctga gggagttgtg acgccaggaa gatcctcgat
     4441 gcgcttacga acacgctcgg caggcaggtc gcgagcaaca cgggccagct tgacaattat
     4501 gaacatatgc tcataattcg ttcagaggcc atccgacatg ccgcgtccgt tctgccaccg
     4561 tcacgttgcc ggccagccgg ccgcgccggt cttcaaaccg gccggcgtgc cggcactgct
     4621 gctcggggac gtggtcatga cgctcgacga gttcgaggcg ctccggctcg cggacctgga
     4681 gaggctgtac cacaagcgcg ccgccgagca gatgggcgtg tcgaggccga cgttctcgcg
     4741 gatcgtcgat tcggcccgcg tcaagatcgc cgacgcgatc gtgcatggca aggcgctcag
     4801 gatagaaggc gggcccgtgc ggctcctggc gggcccacgc cgctgctgcc gtctgcacga
     4861 gggccgcagc cggccgccga cgccggcagg cgccgacggc catcacccgc gcactccgaa
     4921 ggaggaaacc acgtgaggat ctgcattccc gttgacgagg accaggggct tgcgagcccg
     4981 gtctgcctgc atttcgggtc ggcgccgtac ttcatgatcg tgaacaccga cgatggaagc
     5041 tgccgggcca tcccgaaccg gaacgaacac cacgcgcacg gcatgtgcca gccgcttcag
     5101 gccattcagg gcgaggccat cgatggcatg gtggtgggcg gcatcggaat gggggccctg
     5161 aacaagctca gggcggccgg gatctgcgtc tatcaggcgc agcacccgac ggtggacctc
     5221 gcggtcgagg ccatcaaggc cggacggctg cccctcgtgg agcccggctc ggcctgcggg
     5281 ggtcaccaca gccacggacc gggcggccac catcaccggt cctgattcca gcgcaccgcg
     5341 gccgccggcc gggccgggcg ggcttccctg gagccggccc gcccggcgat ccgggagttg
     5401 gggggtgcat cagccgggcc agccagcgta tcctgtggac ggcgcgccgg gtcgcgcgcc
     5461 tgcggccctg gtggcacgcg gtcactccga aggagcacgg cacatggaca agcagaagag
     5521 catcgatctc ctgaacaaag gcgtcgcgga cgaactgcag gcggttcatc agtacatgta
     5581 ctggcacttc catctcgacg atcagggatt tgggccgctg agctccatgt tcaagcggat
     5641 cgccatcgag gagatgggcc acgtggagcg gctcgctgag cgcatccttt tcctgaaagg
     5701 cgacgtcgac atggtgccgg ccggccccgt cgagcgggtg ctcgagccgg acgccatcct
     5761 gaagcgcgcc gcggagatgg agcacgcggc ggtcggcgcc tacaacgcct tcgcgatcca
     5821 gtgcagccag aacgcggacg cggtctcgaa gcagatgttc gaggcgctcg tcggcgacga
     5881 agagcggcac ttcgacgagt tcgacaagca gctcgacaac atcaagcggt tcgggccgag
     5941 ctacctggcc ctgcagtcct tcaacaccgc gccggaatcg gcggcgccgc cgaaggccga
     6001 ctgacggccg gccgggccgc cggcgtgttc ggatgacgga gggcgacacc atggctgagc
     6061 cagtccgcgt cgggatcatc atctgcgacc gctaccgcac gtgcgctggc gggaagtgcc
     6121 tgcgcgccct caaggcccgc gagggggcct tcagcgcgta ccgggggcgc gaggtggagc
     6181 tggtcggctt cacgacctgc aacggatgcc cgggcgggaa cgtggagtac gcgcccggcg
     6241 agatgaagag gaacggggcc gacgtcatcc acctggcgac cggcctcgtc gtcggctatc
     6301 ccccgtgccc gcgcatcgac gcgttcacga cgttcatcgc gaagaagtac ggaatcccgg
     6361 tggtcatcgg aacgcacccg atacccgaga agtactacct gacgcaccag gcctgcggga
     6421 cctggagcgg gccggactgg ccggcgcgcc tcgcgccgac cctcgcggac cgggcggtca
     6481 ggctggccta cgactgatcg cgacgggcga tccactcacg cgggagccgc catgcaggcc
     6541 aagcagccgg gcatcaacag caggctcgtc cacggcacgt accgtcccga cgcgacggga
     6601 gcggtgaacg tccccatcta ccagagctcg acgttcgcct tccggaatgc cgagcacggc
     6661 gcggcgctgt ttgccgggcg ggaaaagggc tacatctaca cgcgcatcgg gaaccccacg
     6721 atcggcgcct tcgaggacgc ggtggcgtcg attgagaacg gcgccggc
//
LOCUS       DAOSIF010000117         6652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10462_length_6652_cov_4.034258, whole genome shotgun sequence.
ACCESSION   DAOSIF010000117 DAOSIF010000000
VERSION     DAOSIF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10462_length_6652_cov_4.034258"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1544
                     /locus_tag="PLT35_05470"
     CDS             177..1544
                     /locus_tag="PLT35_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HOG28711.1"
                     /translation="MLDAIRRAVFWASDFVWGPWSTPVGPVPGPLMAVLILAGIYLTI
                     RTGFVQVRRFPEAARTIVPRQAAGATGALSPFQAFMTALAASIGTGNIAGVATAIVSG
                     GPGALFWIWVYGFLAMAIKFAEAVLGVRFRISAPGGSSSGPMYYLRDGLGMPRLAAVF
                     AGIGGLAVLFTTPFAQPNSMAVVLRSEFGVPTWLAGVAIAALTWLVVIGGVTSIGRAA
                     ERLSPLKVGLYLAGGLFVIASHAGQLPAVLALVVREAFSLQAAAGSTAGVGIIIAMRY
                     GIARGVYANEAGYGTAAVAYGAARSKTPTQQGLNAIIEVFVISFVTSSISALTILVSG
                     VWQSGLSSTAVVAQAFNTAIPVAGGWVVAFCAFLFGYTTLIGWAFYGEQFLEYLFGTR
                     IIVPYRWVYCLLIPVGAVASVEAVWAWGDLMNGLQVFPNLVGVLGLGGFAAAAARCSV
                     TKSGD"
     gene            complement(1525..2841)
                     /gene="thrC"
                     /locus_tag="PLT35_05475"
     CDS             complement(1525..2841)
                     /gene="thrC"
                     /locus_tag="PLT35_05475"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG28712.1"
                     /translation="MRLASTNGRAPIVGLREAVRQGLAPDGGLYMPVDWPRIDPAELD
                     GWRGCAFSDLAARLSARIFEHDFDPGVASRLARAALDFPVLVRRLREGLILLELFHGP
                     TLAFKDIGARFLGRLFGLLHEERGETATVLVATSGDTGSAVARGFAGVRGTRVVLLYP
                     AGRVSAFQESQMATIGGNVEAVRVDGSFDDCQRLVKGALADPELRPLRLTSANSINVG
                     RLIPQVFSYCWGALTVVPPGGAPPVFSVPSGNLGNLTAGVLASRLGLSVAGFTAATNE
                     NDVLPEFLSSGAYRPRQAVATVSNAMDVGDPSNFVRLAALFGGSVARMRAFIRGERVS
                     EAETRRTIRRTFDEDGVVLDPHTAVAVAAARRGPPAAGGGPTIALATAHPAKFADVIA
                     EELGFEPELPEAERGWREKPLLARDLGGASPAAFRKLLEEISLRSW"
     gene            complement(2843..3790)
                     /locus_tag="PLT35_05480"
     CDS             complement(2843..3790)
                     /locus_tag="PLT35_05480"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOG28713.1"
                     /translation="MQRQRVRVFAPATVSNVGPGFDVLGFALHRPGDWVEAEASDRPG
                     VELLEVTGDGGALPVDARRNAAGVAARRLWRQARGGRPAGLRMRLQKGTPLASGLGSS
                     GASAAAGAMAANQLLECPLPLETLVACAMDGERAACGSAHADNVAPSLAGGIVLIRSY
                     LPLELVQLPVPDGLFVAVVHPHCRVSTGEARARLAGRAFPLPDIVANAGNLAALVAAL
                     YRGDLELLGRAIEDRLVEPVRAAMIPAYDAVRRAARDEGALACAVSGSGPSMFAVADA
                     HDAAAAAASAMRRAFRAGASLASDAWVGPISRRGAAPVE"
     gene            complement(3964..6183)
                     /locus_tag="PLT35_05485"
     CDS             complement(3964..6183)
                     /locus_tag="PLT35_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG28714.1"
                     /translation="MNAPARALAVAGLLAALSVPLDGAGPARKLFTYEQVFALPAGPP
                     GAVGAGAGILGRLPDIQGWLDDGHYLERREDPADKQMKTYAVSVADGSAAIHTADAAG
                     PGAARGMSVPGGRWTLSVRDGDLYATDGRTKSLKRLTATADPEVNPRLSPDGAWVAYT
                     RANNLYVYDLERGLERQLTSDGSSVVLNGYSSWVYMEEILGRASAYAAFWWSPDSTKL
                     AFMRFDDSPVPVFPIYHADGQHGELERQRYPKAGDPNPWVQMAVATVADGKIVWMDFD
                     PKADHYIAWPFWTPDSKTLTVQWMNRGQDTLRLYNCDPATGRKTMIFEEKQQAWVEFF
                     EDLTYLPSGTGFIIRSNVDGWDHLYLYAADGTLRKRLTSGEWRVDAIVRVDEPAGLVF
                     FTGRPGKSWDSQLMKVGLDGSGLQAITDGGGMHRVQLSPGGKHFIETVSTIASPAVMN
                     LRAIDGSLVRKLGDQKTAAMDDYAWGRGELFTIPSEDGQFQLPASWVLPPDFDARNAK
                     KTYPVIVSIYGGPNAGTVTNSWPALQAHYWAQRGVITIAVDHRGSGHFGKKGVALMHR
                     SLGKWEMADLIAAAKWLRTKPFVAKDRIGITGSSYGGYTTTMALLYGAAHFNYGQAGA
                     SVTDWRLYDSVYTERYMDTPKENPDGYKAGAVLTYADRYTGGLRMTHGTIDDNVHMQN
                     TIQVVDWLVSHDKPFELMLYPDSRHGYQASQRPHSTREAHDFWMRKLLRQEPGRGGK"
     gene            6280..>6652
                     /locus_tag="PLT35_05490"
     CDS             6280..>6652
                     /locus_tag="PLT35_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28715.1"
                     /translation="MPRLTTVLLAAALTPPASLLLSAQVPRLLERVEVSRVVVDVHVL
                     AGDGRPLRGLSAADLRLTVDGQPVPIESVRWITGAAAPSIDPHDGRMRAPAAPAGDDR
                     PQARRVVLLVQQDFDASRMEGL"
BASE COUNT          882 a         2433 c         2319 g         1018 t
ORIGIN      
        1 cggagcctgg acgctgctcc tcggcgcgat cacggcgctg gtgatcgtgc tgtcgggcgt
       61 cctgctccac cggcggaccc agttctcgta ggacgagtcc ggcagccggg cgcgcggccg
      121 ccggagccgg cgcggcccgc aagctccgcg tgccggcatt ctccggggag tcccccatgc
      181 tcgatgccat ccggcgcgcc gtgttctggg ccagcgactt cgtgtggggg ccctggagca
      241 cgcccgtcgg ccccgtgccg gggccgctga tggccgtgct gattctcgcc ggcatctacc
      301 tgacgatcag gaccggcttc gtccaggtgc ggcggtttcc ggaagccgcg cgcaccatcg
      361 tgccccgcca ggccgccggc gccaccggcg cgctgtcgcc gttccaggct ttcatgacgg
      421 cgctcgccgc gtccatcggg acgggcaaca tcgccggcgt cgccaccgcc atcgtgtccg
      481 gcgggccggg cgcgctgttc tggatctggg tctacggctt cctcgcgatg gcgatcaagt
      541 tcgccgaggc cgtcctcggc gtgcggttcc ggatctccgc ccctggcggc agctcgtccg
      601 gcccgatgta ctatctgcgc gacggcctcg gtatgccccg gctggccgcg gtcttcgccg
      661 ggatcggcgg tctcgccgtc ctcttcacca cgcccttcgc ccagcccaac tcgatggccg
      721 tcgtccttcg aagcgagttc ggcgtgccga cgtggctcgc cggcgtcgcc atcgccgcgc
      781 tcacgtggct cgtcgtcatc ggcggcgtca cctccatcgg gcgcgcggcg gagcggctgt
      841 cgccgctcaa ggtcggcctc tacctggcgg gcgggctctt cgtgatcgcc agccacgccg
      901 gccagctgcc ggccgtgctg gccctggtcg tccgggaggc gttctcgctc caggccgcag
      961 ccggcagcac cgctggcgtc ggcatcatca tcgccatgcg atacggcatc gcccgcgggg
     1021 tgtatgcgaa cgaagccggc tacggcacgg cggccgtcgc ctacggcgcc gcgcgcagca
     1081 agacgccgac ccagcagggg ttgaacgcga tcatcgaggt gttcgtgatc tcgttcgtca
     1141 cctcgtccat cagcgccctg accatcctgg tgagcggcgt ctggcagtcg ggcctgagca
     1201 gcacggcggt cgtcgcccag gccttcaata ccgcaatccc cgtcgccggc ggctgggtgg
     1261 tggccttctg cgcgttcctg ttcggctaca ccacgctgat cggctgggcc ttctacggcg
     1321 agcagttcct cgagtacctg ttcgggacgc gcatcatcgt gccgtaccgc tgggtctact
     1381 gcctgctcat tcccgtcggc gccgtggcca gcgtcgaggc cgtctgggcg tggggcgacc
     1441 tgatgaacgg cttgcaggtc ttcccgaacc tcgtgggggt cctcgggctg ggcggcttcg
     1501 ccgccgcggc cgcccgctgc tcggtcacca agagcggaga ctgatctctt cgagcagctt
     1561 ccggaacgcg gccgggctgg cgccgccaag gtccctcgcg agcagcggct tctcgcgcca
     1621 gccccgctcc gcctcgggca gctccggctc gaaccccagc tcctccgcga tgacgtcggc
     1681 gaacttggcc ggatgcgccg tcgcgagggc gatggtcgga ccgccgcctg cggcgggcgg
     1741 cccgcggcgg gcggcggcaa ccgcgacggc cgtgtgcgga tccagcacga caccgtcctc
     1801 gtcgaacgtc cgccgaatgg ttcggcgcgt ctccgcctcc gacacgcgct cgccgcgaat
     1861 gaaggcccgc atgcgcgcga cggacccgcc gaagagcgcg gcgagcctta cgaagttgct
     1921 cggatcgccc acgtccatcg cgttcgacac cgtcgcgacc gcctggcgcg ggcggtaggc
     1981 gccggatgac aggaactcgg ggaggacgtc gttctcgttt gtggccgccg tgaaacccgc
     2041 gacggacagc cccagccgcg aggccagcac cccagccgtc aggttgccga ggttgcccga
     2101 gggcaccgag aacacggggg gcgctccgcc ggggggcacc accgtcagcg cgccccagca
     2161 gtaggagaac acctggggaa tgagccggcc gacgttgatg gagttcgccg aggtcaggcg
     2221 gagcggccgg agctccgggt cggcgagtgc gcctttcacg aggcgctggc agtcgtcgaa
     2281 gctcccgtcg acgcggacgg cctcgacgtt cccgccaatg gtggccatct gcgactcctg
     2341 gaacgccgac acgcgcccgg caggatagag caggacgacg cgtgtgcccc ggacgcccgc
     2401 gaagccgcgc gcgaccgcgc tcccggtgtc gccggaggtg gccaccagaa cggtggccgt
     2461 ttcgccccgc tcttcgtgca ggaggccgaa caggcggccg aggaatcgcg cgccgatgtc
     2521 cttgaacgcc agcgtcggtc cgtggaagag ctccagcagg atgaggccct cgcgcagccg
     2581 gcgaacgagc acggggaagt cgagcgcggc gcgcgcgagg cggctggcca cgccagggtc
     2641 gaagtcgtgt tcgaagatcc gcgccgaaag acgcgccgcg aggtcggaaa acgcgcaccc
     2701 gcgccaaccg tccagctcgg ccggatcgat ccgtggccag tccacgggca tatagagccc
     2761 gccgtcggga gccagtccct gccgcacggc ttcacggagg cccacgatgg gtgcacggcc
     2821 gttcgtgctg gcgagccgca cgtcactcca ccggcgctgc gcctcggcgg ctgatggggc
     2881 caacccaggc gtcggaggcg agggatgcgc cggcgcggaa ggccctgcgc atggccgagg
     2941 cggcggcagc cgccgcgtcg tgggcgtcgg ccaccgcgaa catcgagggg cccgatccgg
     3001 agacggcgca tgccagcgcc ccctcgtccc gggcggcgcg ccgcacggca tcgtatgcgg
     3061 ggatcatggc cgcgcgcacc ggctcgacca ggcgatcctc gatggcgcgc cccagcagct
     3121 cgagatcacc gcggtaaagc gccgccacga gcgcggcgag gttcccggcg ttcgccacga
     3181 tgtccgggag cgggaacgcg cggcccgcga gccgcgcccg cgcctctccg gtcgacacgc
     3241 ggcaatgcgg gtgcacgacg gccacgaaca gtccgtccgg caccggcagc tggacgagct
     3301 ccagggggag gtacgagcgg atgagcacga tcccgccggc gagcgacggc gcgacgttgt
     3361 cggcgtgcgc cgagccgcag gcggcccgtt cgccgtccat cgcgcacgcc acgagcgttt
     3421 cgagcgggag cgggcactcg agcagctggt ttgccgccat ggcgccggct gcggccgatg
     3481 cgcccgagct gccgagcccg ctggcaagcg gcgtgccttt ctgaaggcgc atgcgaaggc
     3541 ccgccggccg gccgccccgc gcctggcgcc acagacgccg cgccgccacg ccggccgcat
     3601 tccgccgggc gtccacaggc agcgcgccgc cgtcgcccgt cacctcgagc agttcgacgc
     3661 ccggccggtc ggaggcttcc gcctcgaccc aatcaccggg ccggtgaagc gcgaacccga
     3721 gcacgtcgaa gcctggcccg acgttggaca ccgtcgccgg cgcgaacact cgcacgcgtt
     3781 gacgctgcat cgtgacgatg gctccccgcc tgcggcaggc ggcgtgccgg ccggctccga
     3841 gcccccaccg gccgccggcg ccgcccgccg aggctgttca gggcggccct tgaagagccg
     3901 gcgggccgaa gggccagctt cggtgcagca cgccccgcct tgccccctcg ctctgcctcg
     3961 ttcctacttc cccccgcgtc ccggctcctg ccgcagcagc ttccgcatcc agaagtcgtg
     4021 cgcctcccgc gtcgagtggg ggcgctgcga tgcctgatag ccgtgccggc tgtcggggta
     4081 cagcatcagc tcgaacggct tgtcgtgcga caccagccag tcgacgacct ggatggtgtt
     4141 ctgcatgtgg acgttgtcgt cgatggtgcc gtgggtcatc cgcaggccgc ccgtgtagcg
     4201 gtcggcgtag gtgagcaccg cgcctgcctt gtatccgtcc gggttctcct tgggcgtgtc
     4261 catgtagcgc tcggtgtaca cgctgtcata caagcgccag tcggtgaccg acgctcccgc
     4321 ttgcccgtag ttgaagtgcg cggcgccgta gagcagcgcc atcgtcgtcg tgtagccgcc
     4381 gtaagacgaa ccggtgatcc cgattcggtc cttggcgacg aagggcttcg tcctgagcca
     4441 cttcgcggcg gcgatgaggt cggccatctc ccacttgccg aggctgcggt gcatcagggc
     4501 cacgcccttc ttgccgaagt gcccgcttcc gcggtgatcc acggcgatcg tgatgacccc
     4561 gcgctgcgcc cagtagtgcg cctgcagcgc cggccagctg ttggtgaccg tgcccgcgtt
     4621 gggcccgccg tagatcgaca cgatgaccgg gtaggtcttc ttcgcgttcc gcgcgtcgaa
     4681 gtccggcggc agcacccagg acgcgggcag ctggaactgg ccgtcttccg acgggatcgt
     4741 gaacagttcg cccctgcccc acgcgtagtc gtccatggcc gcggtcttct gatcgccgag
     4801 cttgcgcacg agcgagccgt cgatggcgcg caggttcatc accgcgggcg aggcgatcgt
     4861 gctgacggtt tcgatgaagt gcttgccgcc gggagagagc tgcacccggt gcatgccccc
     4921 tccgtccgtg atcgcctgca gccccgaccc gtccaggccg accttcatga gctggctgtc
     4981 ccagctcttg cccggacgcc cggtgaagaa aaccagtccg gccggctcgt ccacccgcac
     5041 gatcgcgtcc acgcgccact cgccagacgt caggcgcttg cgcagcgtcc cgtcggcggc
     5101 atagaggtag agatggtccc atccgtccac gttgctgcgg atgatgaacc ccgtgccgct
     5161 cggcaggtac gtgaggtcct cgaagaactc cacccaggcc tgctgcttct cctcgaagat
     5221 catcgtcttg cggccagtcg ccgggtcgca gttgtagagc cgcagcgtgt cctggccgcg
     5281 attcatccac tggacggtca gcgtcttcga gtccggcgtc cagaacggcc aggcgatgta
     5341 gtggtccgct ttcgggtcga aatccatcca cacgatcttc ccgtccgcga cggtcgcgac
     5401 ggccatctgg acccacgggt tcgggtcgcc tgccttcggg tagcgctgcc gctcgagttc
     5461 tccgtgctgg ccgtccgcgt ggtagatcgg gaacacgggc acggggctgt cgtcgaaccg
     5521 catgaacgcg agcttcgtgc tgtccggcga ccaccagaac gcggcatacg ccgacgcgcg
     5581 gccgaggatc tcctccatgt agacccacga cgagtagccg ttgaggacga cgctgctgcc
     5641 gtcgctcgtc agctggcgtt cgaggccccg ctcgaggtcg tacacgtaca ggttgttcgc
     5701 ccgcgtgtag gcgacccagg ccccgtccgg cgacagccgg gggttgacct cggggtcggc
     5761 ggtggccgtc agccgcttca ggctcttcgt tctcccgtcg gtcgcgtata gatcgccgtc
     5821 acgcaccgac agcgtccatc gccctcccgg caccgacatc ccgcgcgccg cgccgggccc
     5881 agcggcgtca gcggtatgga tcgcggccga gccgtccgcc acggacaccg cgtaggtctt
     5941 catctgcttg tctgccggat cttcgcgccg ctcgaggtaa tggccatcgt ccagccagcc
     6001 ttggatgtcc ggcaggcggc cgaggattcc cgcgcccgcg ccgacggctc ccggaggccc
     6061 ggctggaagg gcgaagacct gctcgtacgt gaagagcttc ctggccggcc cggcgccgtc
     6121 gagcggaacc gagagggccg ccagcaatcc ggcgaccgcc aaagcacgtg cgggagcgtt
     6181 catgacacct ccgtaggtac cggccgcgaa gggcgcgacg gctgaatctt ataccgtccg
     6241 gccgtgcgcc gggcaggcgc atccggtaga ctgccctccg tgcctcggct gacgacggtc
     6301 ctcctcgcgg cggcgctgac gcctccggcc tcgctgctcc tgtcggcgca ggtgccccgc
     6361 ctgctcgagc gcgtcgaggt gtcccgcgtc gtggtcgacg tccatgtgct cgccggggac
     6421 gggcggccgc tgcgcgggct gagcgccgcc gacctgcgcc tcacggtaga cggccagccc
     6481 gtcccgatcg agtcggtgcg ctggatcacc ggggcagcgg cgccgtcgat cgatccacat
     6541 gacgggcgga tgagagcgcc ggccgcgccc gctggcgacg atcggcccca ggcccgccgc
     6601 gtcgtcctgc tcgtgcagca ggacttcgac gcctcccgga tggaggggct cc
//
LOCUS       DAOSIF010000118         6650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10466_length_6650_cov_3.747233, whole genome shotgun sequence.
ACCESSION   DAOSIF010000118 DAOSIF010000000
VERSION     DAOSIF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10466_length_6650_cov_3.747233"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLT35_05495"
     CDS             complement(<1..637)
                     /locus_tag="PLT35_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28716.1"
                     /translation="MSAPKLRVAVWATTFGADLAAFCRFLDRRDDVELTVFMKHPGAF
                     KTTAAWRADPLRARVYARDLRAYPRALARRADVAIVDNAVPLLGISRGLFVLWHGFGW
                     KGPDDRRAFAFMRRCIAAWWGDPWKPDSRVRWQCFGEWDRRFRRDVSGFSEDILRVLG
                     SALHDEIVRPPDRERLQPFYPFDIQRRKTAMLAPTWHYGGVFAHWGAEDDVF"
     gene            complement(634..1809)
                     /locus_tag="PLT35_05500"
     CDS             complement(634..1809)
                     /locus_tag="PLT35_05500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28717.1"
                     /translation="MPLRIAYVSDRPFPSFWTDTQQVMKNAEALALAGAEVRLAIPRA
                     YRSIVRPAARRHRALREFYGVSASLDLQDILTIPCTPARLEKWPHALVAQRRAALAGC
                     DLLYSRNLFPAWLAARRRSRVVYETHRRFDAASGLKARAFLDLAGRRELVGVVTNSEY
                     VRESLLRAGVGADRVLSSKLGHDPRDFAARLTKVEACQALALDPRRPLAVYAGHVDAT
                     KGIDALLAIAGLLPDVGFLLVGGSRIASAALAVRARRMGLSNVSTRPRVPPRAVVPYL
                     YASDILINPPSSTLLAFPHTITPIKLYNYLAAGRPIVAGRTPDVAELLTHDVDALLCR
                     PDDPHAAADAIARVLRTPELAARLAARALETSARYTWEERGRRILRFIQARLDEGRS"
     gene            complement(1781..2515)
                     /locus_tag="PLT35_05505"
     CDS             complement(1781..2515)
                     /locus_tag="PLT35_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOG28718.1"
                     /translation="MLAAGRGVRIRGSELVPKPLMKLGGRPLLARTLDALQAAGIERV
                     VVVLGYMAEDVRTVLAGHTWPGMSVQTVFNPAYEKSNGLSVLAASPDAGGRFILVMSD
                     HLLDSRIISMALESDPGDGTLLCVDRKLDRVFDMSDATKVLTQDGLIRDIGKSLAEYD
                     AVDTGVFHCTTALSAAIRDIAARTGDCSVTAGMQALAARGLARAADIGPLLWQDVDTG
                     EMLEEAGRRLSLIEANEDAAAHRVRQ"
     gene            complement(2615..3757)
                     /locus_tag="PLT35_05510"
     CDS             complement(2615..3757)
                     /locus_tag="PLT35_05510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28719.1"
                     /translation="MMLESPLVAVFVTEFLKYSQTFIFDEIRHHERYEVEVFCHRRVN
                     EGLFAYPRVHSLTPASGALGEIEALLYKATAVSPRFLRHLRRLRPNLLHAHFGPGSIY
                     ALPIRQALNVPLVVTFHGYDVPALRGLSRFRPSNWRYALFARTLLRSVDRFLAASTEL
                     ADMLVGLGAPPGRVRVWRTGVEVPPLAPRSGRPGGPVILMAGRFVEKKGFEYGLEAFA
                     SVAGRHPGARLRLIGDGPRRARYERLIRARGIADKVELAGVLPHHETLAEMARADVLL
                     APSVVAPNGDRESGVVVIKEANARGVPAIGTRHGGIPEIIDHEDTGLVVGERDVQALA
                     EGLHTLIASPTLREAMGRRAREKMIRDYEITDCVRALERHYDEVIG"
     gene            3886..4896
                     /locus_tag="PLT35_05515"
     CDS             3886..4896
                     /locus_tag="PLT35_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28720.1"
                     /translation="MTTRRAESTYGLRSLLGWPRAILYSAEVRSNRAWVSRRLTQHDL
                     EVAWLPVGDTDSPGRVLSGGGDVVPGCSVLLTTCDRPEPCRRLLEALAAQPVASDMPI
                     SRIIVLDDASVADYSAVREALARESTVRVDWYRAARRHGRSGFWRTYQFGFDLVRRLA
                     AGPVLFLQDDLDLVPGFWPEAWRTWQAIADPAKAVLSLCAMEDDEVRGRWIAFERVQL
                     PGQPYRLTQWFDLQAFLVEPRFFELLRYQVLAVPAGRWRRRTDASSGVGEQFTRRLWR
                     RGNVYQVVRTLVLHGSEPSLMNPGARSRRALDNYGAPRVPSEPGVSQRGWSASGGPRR
                     RP"
     gene            complement(4847..6025)
                     /locus_tag="PLT35_05520"
     CDS             complement(4847..6025)
                     /locus_tag="PLT35_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOG28721.1"
                     /translation="MREQFLTFGRPLIGQEEVAEVLDTLASGWLGTGPKTKRFETRFA
                     EYVGARHAVGVSSCTAALHLALDLIGVREGDEIITSPLTFPATANVVEHFRARPVFAD
                     VDRGTMTIDAGQVEAAVTPRTRAIIPVHLAGRPCDMPKLLEIAGRHGLTVIDDAAHAI
                     EAAVGRLKIGSAGHMTAFSFYVTKNLVTGEGGMLTTDRAEWAESARVRSLHGIDSDAW
                     KRYGAEGFTPYDTVYPGYKYNMTDMQASLGLHQLARLDASLAVRERHWARYDEAFGDL
                     DEVITPMRQPGIRHARHLYTLLLRLECLTIDRNQFVDELKRRRIGAGVHFTALHLHTY
                     YRERYGYTRGMFPNAEWIGDRTLSLPLSAGLTESDVEDVIGAVRDVVAAHRSPTIPAG
                     "
     gene            complement(6063..>6650)
                     /locus_tag="PLT35_05525"
     CDS             complement(6063..>6650)
                     /locus_tag="PLT35_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28722.1"
                     /translation="EVLAAARFVEKKGLPDAIAAAALAARDVELRLTVAGGPVDSAAS
                     RAETARIEAAAAASGLGDRLRFVGVLTPADLTALAYRSHLFIQPSRTAADGDTEGGAP
                     VALIDLAATGLPAVATLHADIPDVVSDGVGGLLSPEGDVKALAANVVRLGREPELLLR
                     LSLGAAAHVRDAFTADRCAASLRAHYLAAAGEGRR"
BASE COUNT          973 a         2332 c         2371 g          974 t
ORIGIN      
        1 cgaagacgtc gtcctcggcc ccccagtgcg cgaacacgcc gccgtagtgc cacgtcggcg
       61 cgagcatggc ggtcttgcgc cgctggatgt cgaacggata gaacggctgc aaccgctccc
      121 tgtcaggcgg gcggacgatc tcgtcgtgga gggcggaacc gagcacgcgc aggatgtcct
      181 cgctgaaccc gctgacgtcg cgccggaacc gcctgtccca ttccccgaag cactgccacc
      241 tgacgcgcga gtcgggcttc cagggatcgc cccaccaggc cgcgatgcac cggcgcatga
      301 acgcgaaggc ccgccggtcg tcggggccct tccagccgaa gccgtgccag agcacgaaca
      361 ggcctcggga gatcccgagc agcgggacgg cgttgtcgac gatcgcgacg tccgcgcggc
      421 gggcgagcgc ccgggggtag gcccgcagat cgcgcgcgta gaccctggcg cgcaggggat
      481 ccgcacgcca ggccgcggtg gtcttgaacg cgcccgggtg cttcatgaac accgtcagtt
      541 cgacatcgtc gcgacgatcg aggaatcggc agaacgccgc caggtcggcg ccaaaggtgg
      601 tcgcccagac cgcgacgcgg agcttcggcg cgctcacgac cgcccctcgt ccagcctggc
      661 ctggatgaac cgcaggatcc ggcgcccccg ctcctcccag gtgtaccgcg cggacgtctc
      721 gagggcgcgt gccgccaatc gcgcggccag ctcgggcgtg cggagcacac gggcgatcgc
      781 gtctgccgcg gcgtgcgggt cgtcaggccg gcagagcagc gcatcgacgt cgtgcgtcag
      841 gagttcggcc acgtccggtg tccgtccggc gacgatcggc cgcccggcgg ccaggtagtt
      901 gtagagcttg atgggcgtga tcgtgtgagg gaacgccagg agcgtggatg aaggagggtt
      961 gatcaggatg tccgaggcat acaggtaggg cacgaccgcc cggggaggga cgcgcggccg
     1021 cgtggacacg ttggagaggc ccatgcgccg ggctcgcacg gccagcgccg ccgaggcgat
     1081 ccggctgccc ccgacaagca ggaacccgac gtccggcagc agcccggcga ttgcgagcag
     1141 ggcgtcgatg cccttcgtgg cgtccacgtg ccccgcatac acggccagcg gccgccgggg
     1201 atcgagggcc agggcctggc aggcttcgac cttggtgagg cgcgcggcga agtcccgggg
     1261 atcgtgaccc agcttggacg aaaggacgcg atccgctccg acgccggcgc ggagcagcga
     1321 ctcgcgcacg tactcggagt tcgtcaccac gcccacaagc tcccggcgcc cggcgaggtc
     1381 caggaacgcg cgggccttga ggccgcttgc cgcgtcgaac cgccgatgcg tctcgtacac
     1441 cacccggctg cggcgcctgg cggcgagcca ggctggaaac aggttgcgcg aatacagcag
     1501 gtcgcacccc gccagggcgg cacgacgctg ggccacgagg gcgtggggcc acttctcgag
     1561 gcgggccggc gtgcacggga tcgtgaggat gtcctggaga tcgagggacg ccgacacgcc
     1621 gtagaactcg cgcagcgccc ggtgccggcg ggcggccggg cggacgatcg agcggtaggc
     1681 gcgcggaatg gccagccgca cctcggcccc cgcgagggcg agggcctccg cgttcttcat
     1741 cacctgctga gtgtcggtcc agaaggacgg gaacggcctg tcactgacgt acgcgatgcg
     1801 cagcggcatc ctcattggcc tcgatcaggg agagccgccg gccggcctcc tcgagcatct
     1861 cgccggtgtc gacatcctgc cagaggagcg ggccgatatc ggccgctcgg gccagccccc
     1921 gcgcggccag cgcctgcatc ccggcggtca ccgagcagtc tccggttcgc gccgcgatgt
     1981 cccggatcgc cgcgctgagc gccgtcgtgc agtggaagac gcccgtgtcg accgcatcgt
     2041 actcggcgag gctcttgccg atgtcccgga tgaggccgtc ctgcgtcaag accttggtgg
     2101 cgtcactcat gtcgaacacc ctgtcgagct tccgatccac acagagcagt gtcccgtcgc
     2161 cggggtcgga ctcgagcgcc atcgagatga tgcgggagtc gagcagatgg tccgacatga
     2221 ccaggatgaa ccggcctccg gcgtccggcg acgccgcgag caccgagagc ccgttggact
     2281 tctcgtaggc ggggttgaac accgtctgca cggacatgcc cggccaggtg tggccggcca
     2341 gcaccgtccg gacgtcttcg gccatgtagc ccagcacgac gacgacccgc tcgatgccgg
     2401 ccgcctgcag cgcatcgagc gtcctggcca ggagcggccg tcccccgagc ttcatgaggg
     2461 gcttcggcac cagctccgag ccgcggatgc gcacgccgcg ccccgcagcc agcacgaccg
     2521 ccaccttctc gccgtctcgc agtctcgacc tcctgatgcc gtgcccggat gcggccctgc
     2581 ggctcgctgc cggccgacgc gagggcccct catgttaccc gatcacttcg tcgtagtgcc
     2641 gttcgagcgc cctcacgcaa tcggtgatct cgtagtcgcg gatcatcttc tcgcgcgccc
     2701 tgcggcccat cgcctccctg agcgtcggac tggcgatcag cgtatgcagg ccctccgcga
     2761 gcgcctgcac atcgcgttcg ccgacgacca agcccgtgtc ttcgtgatcg atgatctcgg
     2821 ggatgccgcc gtgacgtgtg ccgatggccg gcaccccgcg cgcgttcgcc tccttgatca
     2881 ccacgacccc tgactcccgg tctccgttgg gtgccacgac gctcggcgca aggagcacgt
     2941 ccgcgcgcgc catctcggcg agggtctcgt gatgcggcag gacgcctgcg agctcgacct
     3001 tgtccgcgat gccgcgcgcc cgaatgaggc gctcgtagcg ggcgcggcgc ggtccgtcgc
     3061 caatgagtcg aagccgagcg ccgggatgcc gcccggccac gctcgcgaac gcctcgaggc
     3121 cgtactcgaa tcccttcttc tccacgaacc gccctgccat caggatgacc ggcccccccg
     3181 gccgtccgga gcgcggcgca agtggcggga cctcgacgcc cgtcctccag acccgcactc
     3241 ggccgggagg cgccccgagg ccgaccagca tgtccgcgag ctcagtcgag gcggccagga
     3301 agcggtcgac gcttcgcagc agcgtcctcg cgaagagcgc ataccgccag ttcgacgggc
     3361 ggaaccggct cagcccccgc agcgcaggca cgtcgtagcc gtgaaaggtc acgactaggg
     3421 ggacgttcag ggcctgcctg atcggcaggg cgtagatgct cccggggccg aaatgcgcat
     3481 ggaggaggtt tgggcggagg cgcctcaggt gccgcaggaa ccgcgggctc accgccgtcg
     3541 ccttgtagag cagcgcctcg atctcaccaa gcgcaccaga ggcgggggtg aggctgtgca
     3601 ctcgaggata ggcgaagagg ccctcgttga cccggcgatg gcagaagacc tcgacttcgt
     3661 agcgctcgtg atggcggatc tcatcgaaga tgaacgtctg gctgtacttc aggaactcgg
     3721 tgacgaacac cgccacgagc ggagattcaa gcatcatcgt ctctggaccg cggaccctcg
     3781 gtccaggcca tcgccggggc cgcaccgagg attgtggcaa cgccggtcgg gtttttccag
     3841 taccatccgg ccgtccgccg tgtgcggcgg agcgctcgca ccaggatgac aacgcgccgc
     3901 gccgaatcga cctacggtct gcgcagcctg ctcggctggc ctcgggccat cctctactcg
     3961 gccgaagtgc gctccaatcg ggcctgggtc agccgccgcc tgacgcagca cgacctggag
     4021 gtcgcgtggc ttccggtcgg ggacacggat tcgccaggcc gcgtgctgtc cggcggcggg
     4081 gacgttgtgc ccggatgctc cgtgcttctc acgacgtgcg acaggccaga gccgtgccgc
     4141 cgtctgctgg aggccctggc cgcacagcct gtcgcgtctg acatgcccat ttcgcggatc
     4201 atcgtgctcg acgacgcgtc ggtggccgac tactcggcgg tccgcgaggc gctcgctagg
     4261 gaatccaccg tgcgggtcga ctggtaccgg gccgcgaggc ggcacggccg gtccggcttc
     4321 tggcgcacgt accagttcgg attcgatctc gtccggcgcc tggcggccgg tcccgtgctg
     4381 ttcctccagg acgacctcga tctcgttccg ggtttctggc ccgaggcgtg gcgtacgtgg
     4441 caggccatcg ccgacccagc caaggcggtg ttgtccctgt gcgccatgga ggatgacgag
     4501 gtgcggggac gctggattgc gttcgagcgc gtgcagctgc cgggccagcc gtaccgcctg
     4561 acccagtggt tcgacctgca ggcgttcctg gttgagcccc ggttcttcga attgctgcgc
     4621 taccaggtgc tggccgtgcc cgcgggccgg tggcggcggc gtaccgacgc ctccagcggc
     4681 gtcggcgagc agttcacccg gcgcctgtgg cgccggggga acgtctacca ggtcgtccgg
     4741 acgctcgtcc tgcacggctc cgagccgagc ctgatgaacc cgggcgcccg ctcgcggcgc
     4801 gcactcgaca actacggcgc ccctcgcgtt ccctccgaac ccggcgtcag ccagcgggga
     4861 tggtcggcga gcggtgggcc gcgacgacgt ccctgacggc gccgatgaca tcctcgacat
     4921 ccgactccgt gagtcccgcc gacaagggaa gggagagggt ccggtcgccg atccattcgg
     4981 cgttggggaa catgccgcgg gtgtagccgt aacgttcgcg gtagtaggtg tgcaggtgca
     5041 gcgcggtgaa atgcacgccg gctccgatcc tgcgcctctt gagctcgtcg acgaactggt
     5101 tccgatcgat cgtcaggcat tcgagccgga ggagcagcgt atagaggtgc cgcgcgtggc
     5161 ggatgccggg ctgccgcatc ggcgtgatga cttcgtcgag gtcgccgaac gcctcgtcgt
     5221 aacgcgccca gtgccgctcg cggaccgcca ggctcgcgtc gaggcgggcg agctggtgca
     5281 ggccgagcga tgcctgcatg tccgtcatgt tgtacttgta gccgggatac accgtgtcgt
     5341 acggcgtgaa gccctccgcg ccgtaccgct tccaggcgtc gctgtcgatc ccgtggaggc
     5401 tcctgacgcg cgcggactcg gcccactccg cccgatcggt cgtcagcatg ccgccttcgc
     5461 ccgtgacgag gttcttggtg acgtagaagc tgaacgcggt catgtggccg gcgctgccga
     5521 tcttgagccg gccgacggct gcctcgatgg cgtgggcggc gtcgtcgatg accgtcaggc
     5581 cgtggcggcc ggcaatctcc agcagcttgg gcatgtcgca gggccgcccg gcgagatgga
     5641 ccgggatgat cgcgcgcgtt ctcggcgtga cggcggcctc cacctgcccg gcgtcgatcg
     5701 tcatggtccc gcgatcgacg tcggcgaaga ccggccgcgc ccggaagtgc tcgacgacgt
     5761 tggcggtggc cgggaaggtc agcggcgacg tgatgatctc gtcgccctcg cggacgccga
     5821 tgaggtcgag ggccaggtgc agcgcggccg tgcacgagga gacgcccacg gcgtggcggg
     5881 cgccgacgta ctcggcgaac cgcgtctcga accgcttcgt cttggggccc gtgcccagcc
     5941 agcccgatgc gagcgtgtcg aggacctcgg cgacttcttc ctggccgatg agaggccgac
     6001 cgaaggtgag gaactgctcg cgcacgtgac ccgtcctccc ggcagcccgc gcgcccgtcc
     6061 cgttaccggc ggccctcgcc ggcagcggcc aggtaatggg cgcgcaggga cgccgcgcag
     6121 cgatcggcgg taaaggcgtc ccgcacgtgg gccgccgcgc cgaggctcag ccgcaacaat
     6181 agctcaggtt cgcggcccag tcgaaccacg ttcgcggcga gggccttcac gtcgccttcc
     6241 ggggaaagca ggccgccgac gccgtcgctg acgacgtcgg ggatgtcggc gtggagcgtc
     6301 gcgacggcag gaagccccgt ggccgccagg tcgatgagcg ccaccggggc gccgccttcg
     6361 gtgtcgccgt cggccgcggt ccgggacggc tggatgaaca ggtgggagcg gtaggccagc
     6421 gccgtgagat cggcgggggt gagcacgccg acgaagcgaa gccggtcccc gaggccgctc
     6481 gcggcggccg ccgcctcgat gcgcgcggtc tccgcccggc tggcagccga gtcgaccggg
     6541 ccccctgcga ccgtcagcct cagttccaca tcccgcgcgg cgagcgcggc ggcggcaatc
     6601 gcatctggca gccccttctt ctcgacgaac ctcgcggcgg ccaggacttc
//
LOCUS       DAOSIF010000119         6628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10512_length_6628_cov_4.627111, whole genome shotgun sequence.
ACCESSION   DAOSIF010000119 DAOSIF010000000
VERSION     DAOSIF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10512_length_6628_cov_4.627111"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLT35_05530"
     CDS             complement(<1..867)
                     /locus_tag="PLT35_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOG28723.1"
                     /translation="MASIPTLKRSGGQVLIMTALTLVVLLALSALTIDLGMAYAVKAK
                     LNSAVDAASLAAGKAVKIGADDATRRANARAAANSFFAANFPTGIRSTSGTTFSVDSI
                     TNVEGTWTVRVSASTVAPTFFSRLFFMLPIRVGASAEATVKTLDMMLVLDCSGSLGSD
                     FPALRDAAKNFINGFQDGPGGDRVGLVTFASGAVLSEAINKDANRGFSKSSVIADLNA
                     TSMGGATNAEEAMRIAKAELDAIPVALRSTLRAIVFFSDGAPNIVSGKFKLKNPAGDR
                     DGIELRLGDSHRL"
     gene            complement(897..1424)
                     /locus_tag="PLT35_05535"
     CDS             complement(897..1424)
                     /locus_tag="PLT35_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOG28724.1"
                     /translation="MRARLTRRIRRAPDERGQALIEMAITLMLLVILMSGVYDYSRAI
                     HAQSVITNMSREGANLIARANQNLSGDEDIDFQNVLDLIGKTAQPLDMVNKGMMYIYK
                     VERVNNRNAITRSLAWNRSAANPKPPRSPSAQNLGGITLAAGQTAYAVEVYYRYTSIF
                     LGRRYEPTLSSISIF"
     gene            complement(1427..1888)
                     /locus_tag="PLT35_05540"
     CDS             complement(1427..1888)
                     /locus_tag="PLT35_05540"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOG28725.1"
                     /translation="MRRRDNGQTLVEFAVVAVVFFTTLFAIIDFAMMFYVNQTMQHAV
                     RAGSRMAIVNPGPNCRAAIIANIKTQSMGYYDKNVNDTKTPTISRQTLGAYGDINGVP
                     INDGSCGTPQMPITVSLTYAWPVMSPFLKPFFANGRYTFTVKATVVNEPQR"
     gene            complement(2228..3718)
                     /locus_tag="PLT35_05545"
     CDS             complement(2228..3718)
                     /locus_tag="PLT35_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28726.1"
                     /translation="MSIPQVKAARLPGVLLAVMVAAVLAFAWSGTAADPDLWGHVRFG
                     QDILAARAIPRADPYSVTSDRPWINHEWLSEAIMAAAYGLGGSAGLVGLKLVIAAVAF
                     AILWRALRRAGVHPPAVAGLVVAGAVGAYGLMLTVRPQLVSMLLYVVLLALLNEAWRG
                     RCRALLWMPLLFAAWANAHGGWIVGIGVLAAWGACAAVAGRVPPAWAAGSVILALAGT
                     LVTPYGTGLWRFLWETVGFGRADITEWQPASADAAFLAAWAAAASLVAIAWHLGRRAA
                     LPLLVPAILVGVAAFRVSRLGGFFALTNVLLLAPCFAGRGPARLPLSRAPTARDLLAV
                     GALCLAGLAAIAAAIQGRIGCVTLALPGPDEQWAPEAQAVQFLRDNPIRGRLLTYFDY
                     GEIAIWHLAPRVRVSYDGRRETVYSEAVQQAHRRFYSKSPNAEYARQLGADYIWLPHR
                     LPVVGLLERDGWVAIFRGARSIVLARAEGAYTQPDPWTGLRCFPGP"
     gene            complement(3803..5626)
                     /locus_tag="PLT35_05550"
     CDS             complement(3803..5626)
                     /locus_tag="PLT35_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG28727.1"
                     /translation="MRIVGQVRAQSGRSVSAVRFWVDGALVGTVTAGPPYAVEWTDDN
                     PFQRREILVEAEDTGGGIGRDRIVLEPYVITELAEITSVLVEAGVYEKNGRMVRGLTA
                     PEFVLEEDGVRQKPDMVAQETVPTTFALLVDSSQSMGRNMDFVRAAAARLVAYLRPKD
                     HVLVAPFSRRLRAITGPTNDPRTINEAVAAIDARGGTAMRDSMIAIAGRFEGIEGRRV
                     IVLITDAYDEHSESSLEEAVAALKEAGVTVYTVGIGGVAGISLKGHDELKAIAAGTGG
                     RAFFPARPIDLPGVYDVLASDAQLRYLVTYTPSNQKRDGTWRAITLRTYLEEYLVKAR
                     SGYFAPQPPPVRPVLEFTALDTHSRYLEISREDLVVIEDGVEQKVETFQEATAPVSIV
                     LALDSSGSMRRASEQVVAAAASFVEALRPEDSLGVLTFADKVNLAHAITTNRANTIDT
                     LAGYRAEGGTALYDALCDALLMLKGLKGRRAVVVLTDGRDEDNPGTGPGSVRTWESVL
                     RLLQDQDVTMFPVGLGTKIDPDRLNLLATFSGGQAYFPQELADLTAEFQRVIENLRHR
                     YVVGYTSTNSARDGKWRSVEIRARSAGIRITSRKGYFAPAR"
     gene            complement(5848..>6628)
                     /locus_tag="PLT35_05555"
     CDS             complement(5848..>6628)
                     /locus_tag="PLT35_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28728.1"
                     /translation="LLCGEIAVPHALVVEGAIIRFYDPVNHEALPESPAEEDAGPLDR
                     DYIPDARWVRCRRPSITAGGELTLDSLDLSFDSVSGFYRAPLQALANGGVLVIDDFGR
                     QHCSARDLLNRWIVPLESRVDYLTLQTGQKFELPFVVLVVFATNLKPAELVDEAFLRR
                     IQYKVFAESPTHADFIQIFGNYCAQQSLEFDRALAERLLVEYFRPRMIPLRGCHPAAL
                     IDQALARAEYWGRPRALTMDLLLAACESYFVDDRELLAEYA"
BASE COUNT         1012 a         2290 c         2273 g         1053 t
ORIGIN      
        1 gaggcgatga gaatcgccaa ggcggagctc gatgccatcc ctgtcgcccg ccgggttctt
       61 gagcttgaac ttccccgaca cgatattcgg cgcgccgtcg ctgaagaaga caatcgcgcg
      121 aagcgtcgac cggagggcga cagggatggc atcgagctcc gccttggcga ttctcatcgc
      181 ctcttccgcg ttggtcgctc cgcccatcga cgtggcgttg aggtcggcaa tcacgctcga
      241 cttgctgaag ccccggttcg cgtccttgtt gatcgcctcg ctcagcacgg ccccggaggc
      301 gaaggtcacc agcccgaccc tgtcgccccc gggtccatcc tggaagccgt tgatgaagtt
      361 cttcgccgcg tctctcagcg cggggaagtc cgaccccagc gagcccgagc agtcgaggac
      421 gagcatcatg tccagcgtct tgaccgtggc ttcggccgag gcgccgaccc ggatcggcag
      481 catgaagaac agccggctga agaacgtcgg cgcgaccgtc gacgccgaga cccggacggt
      541 ccaggtgcct tcgacgttcg tgatgctgtc gacgctgaag gtggtgccgc tcgtcgagcg
      601 gatgcccgtg gggaagttcg cggcgaagaa gctgttggcc gcagccctgg cgttggccct
      661 ccgcgtggcg tcgtcggccc cgatcttcac ggcttttccc gcggccaggg acgcggcatc
      721 gacggcggag ttgagcttcg ccttgacggc ataggccatc cccaggtcga tggtcagcgc
      781 ggacagcgcg agcaggacca ccagggtgag ggcggtcatg atcaggacct gtccgcctga
      841 gcgcttcagc gtcggtatgg atgccatggc gattcctcgg cggcctcaac ggggcgtcag
      901 aagatcgaga tggagctcag cgtcggctcg tagcggcggc cgaggaagat gctcgtgtac
      961 cgatagtaga cctcgacagc gtaggccgtc tggcccgcgg caagcgttat gcctcccagg
     1021 ttctgcgccg acgggcttcg gggcggcttc gggttggctg cgctcctgtt ccacgcgagc
     1081 gatcgggtga tggcgttccg gttgttgacc cgctcgacct tgtagatgta catcatcccc
     1141 ttgttcacca tgtcgagcgg ctgcgctgtc ttgccaatca ggtccaggac gttctggaaa
     1201 tcgatgtctt catcgccgga caggttctgg ttcgcccgcg cgatgaggtt ggcgccttcc
     1261 cggctcatgt tcgtgatgac gctctgggcg tggatggccc gcgagtagtc gtacaccccg
     1321 gacatcagga tcacgagcag catcagcgtg atggccatct cgatgagcgc ctggccgcgc
     1381 tcgtcgggcg cgcggcggat gcgacgggtg aggcgtgctc gcatggctac ctctgcggct
     1441 cgttgacaac ggtggccttg accgtgaagg tgtaccgccc gttggcgaag aacggcttca
     1501 ggaacggcga catgaccggc caggcgtaag tgaggctcac ggtaatcggc atctgcggcg
     1561 tgccgcagct gccgtcgttg atcgggaccc cgttgatgtc gccgtacgcc cccagcgtct
     1621 ggcggctgat ggtcggcgtc ttcgtgtcgt tgacgttctt gtcgtagtac cccatcgact
     1681 gcgtcttgat gttggcgatg atggccgcgc ggcaattggg gccggggttc acgatggcca
     1741 tgcgggatcc cgccctgacg gcatgctgca tcgtctggtt gacgtagaac atcatggcga
     1801 agtcgatgat ggcgaacagg gtcgtgaaaa agacgaccgc gaccacggcg aattcaacca
     1861 gcgtctgtcc gttgtctcgc ctgcgcatcg ggctacctcg tgggcggcac tcctccccgt
     1921 ggaacttaag caagaatcct gcctgacgcc gcggcacgca ccgcgatccg agctgctatg
     1981 gcggccggtc gcgaggctcg cggcgaaggc cggatctatc gtaccaagtc ctttatttcc
     2041 atactcttat cgggggattc aacagcacgg cgtagacggc gcatccagcc gcggcgcccg
     2101 gccgcacgcg gcgtttcaac atttgtccct tctacggcag acttgtctcc gctctctgcc
     2161 agaatccgcg gggcatctgc ccccggttgc tgcatccgct ggaggcaggc gcctccgcga
     2221 taccgcgtca cgggcctggg aaacaccgca ggccggtcca cgggtccggt tgagtatacg
     2281 ccccctccgc tcttgcgagg acgatggacc tggcgccccg gaagatcgcc acccacccgt
     2341 cgcgctcgag caggccgacg accggcagcc ggtgcggcag ccagatgtag tcggccccca
     2401 gctgccgcgc gtattccgcg ttcggcgact tcgagtagaa ccgccggtgc gcctgctgca
     2461 cggcctccga gtacacggtt tcgcgcctgc cgtcgtacga cacgcgcacc ctcggcgcaa
     2521 ggtgccagat ggctatctcg ccgtagtcga aatacgtgag caatcgtccg cgtatcgggt
     2581 tgtcgcggag aaactgcacc gcttgcgcct cgggcgccca ttgctcgtcg ggccccggca
     2641 gggcgagggt cacgcagcca atccgtccct ggatggcggc agcgatggcc gcgagtcccg
     2701 ccaggcagag cgcgcccacg gcgaggaggt cgcgcgccgt cggcgcgcgc gacagcggca
     2761 ggcgggcggg gcctcggccg gcgaagcagg gcgccaggag cagcacgttg gtcagcgcga
     2821 agaaaccgcc gagcctgctc accctgaacg ctgcgacgcc gacaaggatg gcgggtacga
     2881 gcaggggcag tgcggcgcgc cggcccagat gccaggcgat cgccacgagc gaggcggccg
     2941 ccgcccatgc cgccaggaac gccgcgtcgg cgctcgccgg ctgccactcg gtgatgtccg
     3001 cgcgtccgaa gccgacggtc tcccacagaa accgccacaa gccggtcccg tacggtgtca
     3061 cgagcgttcc tgccagagcc aggatcaccg atccagccgc ccacgccggc ggcacgcggc
     3121 cggcgacggc ggcgcaggct ccccacgccg ccagcacgcc aatcccgaca atccatcccc
     3181 cgtgcgcgtt ggcccaggcc gcgaagagca gcggcatcca gagcagcgca cggcagcggc
     3241 cgcgccaggc ctcgttcagc agcgcgagca gcacgacgta gagcagcatc gagacgagct
     3301 gaggccggac ggtcagcatc aggccgtagg cgcccacggc gcctgcgacg accaggcccg
     3361 cgacggccgg cggatgcacg ccggcccgcc tgagggcgcg ccagaggatg gcgaacgcca
     3421 cggcggcgat gacgagcttg aggcccacca gcccggcgct tcccccgagc ccgtacgccg
     3481 cagccatgat cgcttccgac agccattcat ggttgatcca gggccggtcg ctcgtgacgg
     3541 agtacggatc ggcgcgcggg atcgcgcgcg ccgctaggat gtcctggccg aagcgcacgt
     3601 gcccccacag gtccgggtcg gccgcggtcc cggaccacgc gaacgcgagg acggcggcga
     3661 ccatcacggc gagcagcaca cccgggagtc gcgcggcctt cacctgcggt attgacatgg
     3721 ggctccatcc tcctgggccg gcggctcagc cggggccgca gcctcggccc ccgctcgcgt
     3781 caccgtcccc cgtcgcgccg tgtcatcgcg ctggcgcgaa gtaccccttg cggctggtga
     3841 tgcgaatgcc ggccgagcgc gcgcggatct cgacgctgcg ccacttgccg tcgcgcgcgg
     3901 agttggtcga cgtgtacccg acgacgtagc ggtggcggag gttctcgatc acgcgctgga
     3961 actccgcggt gagatcggcc agctcttgcg ggaagtaggc ctggccgccc gagaacgtcg
     4021 ccagcaggtt caggcgatcc ggatcgatct tcgtgccgag tcccacggga aacatggtca
     4081 cgtcctggtc ctgcagcagg cgcagcacgc tctcccacgt cctgacgctg cccggcccgg
     4141 tgcctgggtt gtcctcgtcg cggccgtcgg tgaggacgac caccgcgcgc ctgcccttga
     4201 ggcctttgag catgagcagc gcgtcgcaca gggcgtcgta gagggctgtt ccgccctcgg
     4261 cccggtagcc ggcgagcgtg tcgatggtgt tcgcgcggtt cgtcgtgatc gcgtgcgcga
     4321 ggttcacctt gtcggcgaag gtcagcacgc caaggctgtc ctcgggacgc agggcctcga
     4381 cgaagctcgc cgcggccgcc accacctgct cgctcgcgcg cctcatgctg ccgctcgagt
     4441 cgagcgccag cacgatcgac accggggcgg tcgcctcctg gaaggtctcg accttctgct
     4501 cgacgccgtc ctcgatcacg acgaggtcct ctctcgagat ctcgaggtac cggctgtggg
     4561 tgtcgagcgc cgtgaactcg agcacggggc gcaccggcgg cggttgcggc gcgaaatagc
     4621 cgctgcgcgc cttcaccagg tactcctcca ggtacgtccg aagcgtgatg gcgcgccacg
     4681 tgccgtcgcg tttctggttg ctcggcgtgt acgtcaccag gtagcgcaac tgcgcgtcgc
     4741 tggccagcac gtcgtacacg ccgggcaggt cgatcgggcg cgccgggaag aacgcccggc
     4801 cgccggtgcc ggcggcaatc gccttcagct cgtcgtggcc cttcagcgag atgccggcca
     4861 cgccgccgat gccgaccgtg tacaccgtga cgccggcttc tttcagcgcc gccaccgcct
     4921 cctcgagcga cgactcgctg tgctcgtcgt acgcgtcggt gatcaggacg atgacccggc
     4981 ggccctcgat cccctcgaag cggccggcga tggcgatcat cgagtccctc atggcggtcc
     5041 cgcccctggc gtcgatggcg gcaacggcct cgttgatggt ccgcggatcg ttggtggggc
     5101 cggtgatcgc ccgcagtcgc cgcgagaacg gcgccaccag gacgtggtcc ttcggccgca
     5161 ggtacgccac caggcgcgcg gccgccgcgc ggacgaaatc catgttccgc cccatgctct
     5221 ggctgctgtc cacgagcagg gcgaaggtgg tgggcacggt ttcctgcgcc accatgtcgg
     5281 gcttctggcg cacgccgtcc tcctcgagga cgaactcggg cgcggtcagg cccctgacca
     5341 tgcggccgtt cttctcgtac acgccggcct cgaccagcac gctcgtgatc tcggccagct
     5401 cggtgatgac gtagggctcg aggacgatcc ggtctctgcc gatgccgccg ccggtgtctt
     5461 cggcctcgac gaggatttcg cgccgctgga acgggttgtc gtcggtccat tcaacggcgt
     5521 acggcgggcc ggcggtcacc gtgccgacca gggcgccgtc cacccagaag cgcacggcgc
     5581 tcacggaccg gcccgactgg gcccgcacct ggccaacgat gcgcaccgtg cccggctcgc
     5641 ccatgcggcc gagcggcgag gtgattctga cggaaacggc ggaaggggac tgggcccaag
     5701 ccgctaccgg cgccagcagg atcgcgagca cgcacgggag cgcgctccgc gggcgcgcgc
     5761 gcccacgccg gcgccgcctg gcctcctcga cctccacgcg ggattggccg cctcggttgc
     5821 cgtcatgcgc cgtcatccat ccacgtctca ggcgtactcc gccagcagct cccggtcgtc
     5881 cacgaagtag ctctcgcacg cggccaggag cagatccatc gtcagtgcgc gcggccggcc
     5941 ccagtactcg gcccgcgcca gcgcctgatc gatcagcgcc gccggatggc acccccgcag
     6001 ggggatcatg cgaggccgga agtactccac cagcagccgc tccgccagcg cccggtcgaa
     6061 ttcgagcgac tgctgcgcgc agtagttgcc gaagatctgg atgaaatccg cgtgcgtggg
     6121 gctctcggcg aagaccttgt actggatgcg tcggagaaac gcctcgtcca ccagttcggc
     6181 gggcttgagg ttcgtggcga agaccaccag cacgacgaac gggagctcga acttctgccc
     6241 ggtctggagc gtcaggtagt cgacgcggct ctcgagcggc acgatccagc ggttcagcag
     6301 atcgcgcgcg gagcagtgct gccggccgaa atcatcgatg acgagcacgc cgccgttggc
     6361 gagggcctgg agcggggcgc ggtagaagcc cgacacggag tcgaagctca ggtcgaggga
     6421 gtcgagcgtc agttcccctc ccgccgtgat cgacgggcgc cggcaccgca cccaccgcgc
     6481 gtcggggatg tagtcgcgat cgagcgggcc ggcgtcctcc tcagcggggc tttcgggcag
     6541 cgcctcgtgg ttcacgggat cgtagaagcg gatgatggcg ccctcgacga ccagggcgtg
     6601 cggcaccgcg atctccccgc agagcagg
//
LOCUS       DAOSIF010000120         6623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10522_length_6623_cov_2.983557, whole genome shotgun sequence.
ACCESSION   DAOSIF010000120 DAOSIF010000000
VERSION     DAOSIF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10522_length_6623_cov_2.983557"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2126
                     /locus_tag="PLT35_05560"
     CDS             <1..2126
                     /locus_tag="PLT35_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28729.1"
                     /translation="QEVDIVLLSAKDGKVIRNLTPGFDQGMGFDYIVTPGSRWITVPW
                     LAWSPSGDRLAYVVRKEKSKSIVVQNVVTRKIEQRIDLKTVDEPESPSFSPDGASLAF
                     AANQDGISDIFTVDLATERVTNVTRDDLANYGPTYAPDGASIVYLSRVSGNEKLFSID
                     LATGRKTQLTFGTHDDAAARFLDEETIVFPSTAMDPDTAIEPEIARNGNAFNLWTLGL
                     KTGELKQWTDALGGNFSPAVLRDGASSRIAFVAYYKGGYELHTLDRKEPVLVAAASDF
                     GAPGPLIDFQAPLSHALVAENKRRKRSWDKLFMEGRPSIALGVTSGSDLFGGTALSFS
                     DVLGDRRIDAMFAEMAQYRMYGVTMINLERRLNYAVQAYWQDLFYYGNVAGVYYDPYY
                     APYIDRDLATSVRSNRGASLYGIYPFDRYRRLEFSGGFGHIAERYTDEALEELARQYQ
                     QAAYGSALYRRGWYVPLTMAFVQETTIFREYGPLSGSTMRLAYEVAPKLGGGTLSWQA
                     VDLDIRKYFKLGSSSLVAVRGRGFRSWGANPSFTYYGGNSDMRGYDYLEFAGQNSVYA
                     NAELRFPLIHAMATPIGVLGGIRGVAFFNVGGAWWSNTGFKFSTTESEVVRPITGYTL
                     DAAGNLGYLYGDPVQVDGFRLVNGRASYGFGLTAFAFGFPVHFDWSWRTLFNKPWEDA
                     VFALSDGGGAKWRRPRFQFWIGYDF"
     gene            complement(2205..3716)
                     /locus_tag="PLT35_05565"
     CDS             complement(2205..3716)
                     /locus_tag="PLT35_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOG28730.1"
                     /translation="MPTTARLPVALSTLLAAVGLVAALRGAPLTDTPEIHLQLGKLLY
                     AEARYGEALTAFRTALASADGRARVAARVGAVQAAMRIGDFAAVRDEAARLRDESRGD
                     PEARAVYGDALWSAGLFAEAEAAFRESLAIDPEQPRGHNGLARALMARSRHDQALEHS
                     LLAVGLEPKEAEFHHTLGNVYERLGRYDAAAGALSSFIDLLPKGIADPRAALAGAELR
                     FLSSFGKKAPYEFISDPDRVYTLPFRLEQDKILVRGRVNGSQPLDFVVDTGAELTTIS
                     RDTGERERVAGVTYTVSAGVGEVGMRVLQLGRIEKLEVGPIKVKRVPCILKSPALREL
                     PAPEGESLSPLAMGLSVGVDYRRRILTLARRLPDDGPADVTLPLWMNRLATVRGTVGI
                     DHPRSFVVDTGGEVISISADTAKVLPPLVTRKIGLKVFGASGWDRDAYLLPGVDLAFA
                     DIRFRRLSVVVLNLRAPSVLLGYDLGGIVGHRFLSRYRVAIDLDRAELRLTSS"
     gene            complement(3814..4278)
                     /locus_tag="PLT35_05570"
     CDS             complement(3814..4278)
                     /locus_tag="PLT35_05570"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOG28731.1"
                     /translation="MLRDLLKKIFLVDLVKGLWVTLKYTPQPAFTFQYPAERRQVSPR
                     FRGVLRLQVDPESGAQVCIVCDQCAKACPDDLLSLGGHREPGMKLKALDYFDFNLSRC
                     SFCGLCSEVCPTKPIKALVMSEDYELGSYSRAAQVLRIDDMYDGLPITQYTR"
     gene            complement(4278..4814)
                     /locus_tag="PLT35_05575"
     CDS             complement(4278..4814)
                     /locus_tag="PLT35_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOG28732.1"
                     /translation="MGVIDHRFGDNVITTNVDAVLAWARKNSLWPVTFGLACCAIEMI
                     AAGGSRFDIARFGAEVFRPSPRQADLMIVAGTVTKKMAPVLRRLYDQMPEPKWVISMG
                     SCSNAGGPFPTYSVLQGVDKVVPVDVYISGCPPRPEALFYGLMRLQDKIAKESTVLRR
                     ERRIPLGGAEPIVIEERG"
     gene            complement(4849..5109)
                     /locus_tag="PLT35_05580"
     CDS             complement(4849..5109)
                     /locus_tag="PLT35_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOG28733.1"
                     /translation="MAYIICEPCIGTKDTACVDVCPVDCIHPRKDEAEFATAEMLYIH
                     PDECIDCGACVPACPVEAIFALDDVPEKWRSFIATNAEYYKR"
     gene            complement(5152..5880)
                     /locus_tag="PLT35_05585"
     CDS             complement(5152..5880)
                     /locus_tag="PLT35_05585"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG28734.1"
                     /translation="MPARSTSIDKALDVLEALSRSPAGAPLTEVARALGLPRPTVHRL
                     LARLKRRGYVRQDDESQRYGLTLKVLDLSFRLLGRSELRLHAYPVLREHARPRRTRSF
                     LAIPSSGEVTYIWSAGSDGAAARTAYGRPMPAHCAEYFAKPAPGARRLSCLRLGAAGG
                     AGGGAPAPLRLGLPDAEGSQRLYCTCAPVYDYSEREVARIGVFGHGPADGVALAECPR
                     EVEDLARRVSMRLGFLPQAGVGGR"
     gene            complement(5977..>6623)
                     /locus_tag="PLT35_05590"
     CDS             complement(5977..>6623)
                     /locus_tag="PLT35_05590"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOG28735.1"
                     /translation="PGGRRVLVDPWLEGNPSCPAGVRVDAADVILVTHGHFDHVHDAA
                     PVAKRTGATVVGIFELCQWLEGMGVEKTSGMNKGGSQDLCGITFTMVDARHSSSFAGE
                     GGAVYLGEAAGYVVTFENGLRVYVAGDTSVFGDMRLIAELYRPTVAFLPIGGHFTMDP
                     VEAARACELLGVRAVIPMHYGTFPVLKGRPAQLRELVAPLGVDVIEAVPGETLS"
BASE COUNT         1038 a         2402 c         2202 g          981 t
ORIGIN      
        1 accaggaagt cgacatcgtg ctcctgtcgg cgaaggacgg caaggtcatc cgcaatctca
       61 cgccgggctt cgaccagggc atgggcttcg actacatcgt gacgccggga agccgctgga
      121 tcacggtccc gtggctcgct tggtcgccct ccggcgaccg cctggcgtac gtcgtccgca
      181 aggagaagtc gaagtccatc gtcgtccaga acgtcgtcac gcggaagatc gagcagcgca
      241 tcgatctgaa gaccgtggac gagcccgagt cgccgagctt ctcgcccgac ggggcgtcgc
      301 tcgccttcgc ggcgaaccag gacgggatct cggacatctt caccgtcgat ctggcgacgg
      361 agcgggtcac caacgtgacc agggacgacc tggcgaacta cgggccgact tacgccccgg
      421 acggcgcgtc catcgtgtac ctctcgcgcg tgagcggaaa cgagaagctg ttctcgatcg
      481 atctggcgac gggccggaag acgcagctca ccttcggcac gcacgacgac gcggcggcgc
      541 gcttcctgga cgaggagacg atcgtctttc cgtcaaccgc gatggacccg gacaccgcaa
      601 tcgagcccga gatcgcgcgc aacggcaacg cgttcaacct gtggacgctc ggcctgaaga
      661 cgggcgagct gaagcagtgg actgacgcgc tcggcgggaa cttctcgccg gccgtgctcc
      721 gcgacggcgc ctcgtcgcgg atcgcgttcg tggcgtacta caagggcggg tacgaactgc
      781 acaccctgga ccgcaaggag ccggtgctgg tcgcggcggc ctccgacttc ggcgcgcctg
      841 ggcccctcat cgacttccag gcgccgctct cgcacgccct ggtggccgag aacaagcggc
      901 gcaagcggtc ctgggacaag ctgttcatgg aaggccggcc ctccatcgcc ctcggcgtga
      961 cgagcggcag cgacctgttc gggggcacgg ccctgtcctt ctcggacgtc ctcggcgacc
     1021 gccgcatcga cgcgatgttc gccgagatgg cccagtaccg gatgtacggc gtcacgatga
     1081 tcaacctcga gcgccgcttg aactacgcgg tgcaggccta ctggcaggac cttttctact
     1141 acgggaacgt cgccggggtg tactacgatc cctactacgc cccctacatc gaccgcgacc
     1201 tcgcgacctc ggtgcggagc aaccgcggcg cgtcgctcta cggcatctac ccgttcgatc
     1261 gctaccggcg cctcgagttc tcgggcgggt tcgggcacat cgccgaacga tacaccgacg
     1321 aggcgctcga ggagctggcc cggcagtacc agcaggccgc ctacgggtcc gcgctgtacc
     1381 gcaggggctg gtacgtgccg ctcacgatgg cgttcgtgca ggagacgacg atcttccgcg
     1441 agtacggccc gctttccggc agcaccatgc gcctggcgta cgaggtcgcc cccaagctgg
     1501 gcggcggaac gctgtcctgg caggcggtcg atctcgacat ccgcaagtac ttcaagcttg
     1561 gctcgtcgtc gctcgtcgcc gtgcgggggc gcggcttccg aagctggggc gcgaacccgt
     1621 cgttcacgta ctacggcggc aactccgaca tgcgggggta cgactacctc gagttcgccg
     1681 ggcagaactc ggtctacgcg aacgccgagc tccggttccc cctcatccac gccatggcca
     1741 cgcccatcgg ggtgctggga ggcatccgcg gcgtggcgtt cttcaacgtc ggcggcgcat
     1801 ggtggagcaa caccggcttc aagttctcca ccaccgagag cgaggtggtc aggccgatca
     1861 ccggctacac gctcgacgcg gccgggaacc tcgggtacct gtacggagac ccggtgcagg
     1921 tggacggctt ccgcctcgtg aacggccgcg cctcctacgg cttcggcctc acggcgttcg
     1981 ccttcgggtt ccccgtccac ttcgactggt cgtggagaac gctcttcaac aaaccgtggg
     2041 aggacgccgt gttcgcgctg tccgacggcg gcggcgccaa gtggcggagg ccccgcttcc
     2101 agttctggat cgggtacgac ttctaggcgg cgcggccgcc gtccgaggcc gggcggcggc
     2161 caacgccccg gggcggatcg ctccctccgg ggcgctggcc cgcctcagga ggaggtcagg
     2221 cggagctcgg cgcgatcgag gtcgatggcc acgcggtacc ggctgaggaa gcggtgcccc
     2281 acgatgccgc cgaggtcgta gccaagcagc acgctcggcg cgcgcaggtt gaggacgacg
     2341 accgacagcc tcctgaaccg gatgtcggcg aacgccaggt cgacgcccgg aaggaggtag
     2401 gcgtcccggt cccacccgga cgcgccgaag accttcaggc cgatcttccg cgtgaccaga
     2461 ggcggcagca ccttggccgt gtccgcgctg atcgagatca cctcgccgcc cgtgtccacg
     2521 acgaagctcc gcgggtggtc gatgccgacc gtgccccgca ccgtcgcgag gcggttcatc
     2581 cacagcggca gggtgacgtc ggcggggccg tcgtcaggca ggcgccgcgc gagcgtcagg
     2641 atccgccgcc ggtagtccac gccgaccgag aggcccatgg ccagcggcga caggctctcc
     2701 ccttccggcg ccggcagctc gcgcagcgcg gggctcttca ggatgcaggg cacgcgcttg
     2761 accttgattg gccccacctc gagcttctcg atccggccga gctggagcac gcgcatgccg
     2821 acctctccga cgccggcgct gaccgtgtag gtgacgcccg ccacgcgctc ccgctcgccc
     2881 gtgtccctcg aaatcgtcgt cagctcggcg ccggtgtcca cgacgaagtc caacggctga
     2941 ctgccattga cgcggcccct gacgaggatc ttgtcctgct cgaggcggaa tggcagcgtg
     3001 tacacccggt ccggatccga gatgaactcg tagggcgcct ttttcccgaa cgacgacagg
     3061 aatctcagct ccgcgccggc gagcgccgcg cgcgggtccg cgatcccctt cggcaggagg
     3121 tcgatgaagc tgctcagggc gccggcggcc gcatcatagc ggcccagccg ctcgtagacg
     3181 ttgccgaggg tatggtggaa ctcggcctcc ttcggctcca ggccgaccgc cagcaggctg
     3241 tgctcgagcg cctggtcgtg gcggctgcgc gccatcaagg cgcgggcgag accgttgtgc
     3301 ccgcggggct gctccgggtc gatcgcgagg gattcccgga acgccgcttc ggcctccgcg
     3361 aacagccccg ccgaccacaa ggcgtcgccg tagacggccc gggcctcggg gtcgccgcgg
     3421 ctctcgtcgc gcagccgggc ggcctcgtcg cgcaccgccg cgaagtcgcc gatccgcatg
     3481 gcggcctgca cggcgccgac ccgggccgcc acgcgcgcgc ggccatcggc cgaggccagc
     3541 gccgtccgga aggccgtgag cgcctcgccg tagcgcgcct cggcgtagag cagcttgccg
     3601 agctgcaggt ggatttctgg ggtgtccgtc agcggcgcgc cgcggagggc cgcgacgagg
     3661 ccgacggctg cgagcagcgt ggacagcgcc actgggaggc gagcggtggt gggcatgcgc
     3721 cctccgtctc cgggacgtcc gaggcgattc gctccggcga gagcggccgc ggcgggcggc
     3781 cggcggcctc ggccgcccgc agtcgtccgc gcgtcagcgc gtgtactgcg tgatgggcag
     3841 cccgtcgtac atgtcgtcga tgcgcagcac ctgggccgcc cggctgtacg agccgagctc
     3901 gtagtcctcg ctcatgacga gggccttgat gggcttcgtc gggcagacct ccgagcacag
     3961 gccgcagaag ctgcaccgcg acaggttgaa gtcgaagtag tcgagggcct tcagcttcat
     4021 gcccggttcg cggtgccctc ccagcgagag cagatcgtcc gggcacgcct tcgcgcactg
     4081 gtcgcacaca atgcagacct gcgcgcccga ctccggatcg acctggaggc gcagcactcc
     4141 gcggaaacgc ggcgacacct gccggcgctc cgcggggtac tgaaacgtga acgccggctg
     4201 aggcgtgtac ttcagcgtga cccagaggcc cttgacgagg tcgaccagga agatcttctt
     4261 cagcaggtcc ctgagcatca gccgcgctcc tcgatgacga tcggctccgc gccgccgagg
     4321 ggaatcctgc gctccctgcg aagcacggtg ctctccttcg cgatcttgtc ctgcagccgc
     4381 atgagcccgt agaacagcgc ctcgggacgg ggcggacagc ccgagatgta gacgtcgacg
     4441 ggcaccacct tgtccacgcc ctgcagcacg ctgtaggtcg gaaacggccc gcccgcattc
     4501 gagcagctcc ccatcgagat gacccacttc ggctccggca tctggtcgta cagccgtcgg
     4561 agcaccggcg ccatcttctt cgtgaccgtg ccggcgacga tcatcaggtc cgcctgccgc
     4621 ggcgacggcc ggaacacctc ggccccgaag cgggcgatgt cgaagcgcga cccgccggcc
     4681 gcgatcatct cgatggcgca gcaggccagg ccgaaggtga ccggccagag agagttcttc
     4741 cgcgcccacg ccaagacggc gtcgacgttc gtcgtgatga cgttgtcgcc gaaccgatgg
     4801 tcgatcacac ccatgacgcc aatccccgcc gctcgtgccg cggccgcatt accgcttgta
     4861 gtactcggcg ttcgtcgcga tgaagctccg ccacttctcc ggcacgtcgt ccagggcgaa
     4921 gatcgcctcc acggggcacg ccggcacgca ggctccgcag tctatgcatt cgtccggatg
     4981 gatgtacagc atctcggcgg tggcgaactc ggcttcgtcc ttgcgcgggt ggatgcagtc
     5041 caccgggcac acgtccacgc acgcggtgtc cttcgtgccg atgcacggct cgcagatgat
     5101 gtacgccacg tcagcctcct cctccgcgcg cccgccaggg ccgcgcgctc gctagcggcc
     5161 gcccaccccg gcctggggca ggaatcccag cctcatcgac acccgcctcg ccagatcctc
     5221 gacttcgcgc ggacactccg caagcgccac gccgtcggcc ggaccatggc cgaacacgcc
     5281 aatccgcgcc acctcgcgct ccgagtagtc atacaccggc gcgcacgtgc aatacaaccg
     5341 ctggctgccc tccgcgtcgg gcaggcccag cctgagcggc gcgggcgccc cgccgcctgc
     5401 gccgcccgcc gcgcccagac gcaggcagct gagccgccgc gcgcccggcg ccggtttggc
     5461 gaagtactcc gcgcagtgcg caggcatcgg ccgcccgtag gccgtccgcg ccgccgcccc
     5521 gtccgaaccc gcgctccaga tgtaggtcac ctcgcccgat gacggaatcg ccaggaagct
     5581 gcgggtccgc cttggccgcg catgctcccg gagcaccggg tacgcatgca gccggagctc
     5641 ggaacgcccc agcagccgga agctcagatc gagcaccttg agcgtcaggc cgtaccgctg
     5701 cgactcatca tcctgccgca cgtagccgcg ccgcttcagg cgcgcgagca gccggtgcac
     5761 cgtgggacgc ggcagaccca gcgcgcgcgc cacttcggtc agcggcgcgc ccgccggcga
     5821 ccggctcagg gcttcgagga cgtccagagc cttgtcgatg gacgtcgatc gtgccggcac
     5881 cgccgcacct tcagcgaaac cccatcatat ggttccacta tacggaatgt tattccagaa
     5941 aatcacacta cctttcgaca gccgtgaagt caagcctcaa gagagcgtct cgcccggcac
     6001 cgcctcgatc acatccacgc cgagcggcgc caccaattcg cgcagctgcg cgggacgccc
     6061 cttcagaacg gggaacgttc cgtagtgcat cgggatgaca gcgcgcacgc ccagcagttc
     6121 gcacgcccgg gcggcctcga cgggatccat ggtgaagtgg cctccgatcg ggagaaacgc
     6181 caccgtcggc cggtacagct ccgcgatgag ccgcatgtcg ccgaagaccg aggtgtctcc
     6241 ggcgacgtac accctcaggc cgttctcgaa ggtgacgacg tagccggccg cctcgcccag
     6301 atagactgcc ccgccttcgc cggcgaagct cgagctgtgc cgcgcatcca ccatggtgaa
     6361 ggtgatgccg cacaggtcct ggctcccgcc cttgttcatg cccgacgtct tctcgacgcc
     6421 cattccctcg agccactggc acagttcgaa gatgccgacg acagtggctc ccgtccgctt
     6481 ggccaccggc gccgcgtcgt ggacgtgatc gaagtggccg tgcgtcacca gaatcacgtc
     6541 cgccgcgtcg acgcgcacgc ccgccgggca agacgggttt ccctccagcc acgggtccac
     6601 caggacccgc cggccgcccg gcg
//
LOCUS       DAOSIF010000121         6620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10527_length_6620_cov_4.205331, whole genome shotgun sequence.
ACCESSION   DAOSIF010000121 DAOSIF010000000
VERSION     DAOSIF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10527_length_6620_cov_4.205331"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLT35_05595"
     CDS             complement(<1..1241)
                     /locus_tag="PLT35_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOG28736.1"
                     /translation="MRAGTRSRRVLTAAAVACAAVMIRAAPASSQAPAAGGRGPAYRL
                     IELPSGRVLAESRPDLLAAPTAPGSIVKLATLLAAVESGIVNEQTRISCRRQILVDGK
                     RLTCVHPDLHRPLSAAEALGHSCNVFFATVAQRLPRQSLDAALVRLGLPPADPDAPTA
                     SAALGLRGVRARPPQLLEAFLRLVGLSPREVALPAAAARILRAGTELAARSGTASALA
                     ASGLRGLAKTGTAPMPGGGAAGLVTAAVDTELPLHAIVVLVPGGAGSDAAVEAARILV
                     RHGAPQRHGEPMVRVGVARRDGRGYDIVTTRLEDYVAQVVTGEMGGSGPSAALEAMAI
                     TARTFALANMGRHRNDGFDLCDLTHCQVLARPTAAADAAARATAGLVLTAGTAPADVY
                     YSSSCGGHTETPSRVWTGARD"
     gene            complement(1247..1933)
                     /locus_tag="PLT35_05600"
     CDS             complement(1247..1933)
                     /locus_tag="PLT35_05600"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOG28737.1"
                     /translation="MATRPRVFYLHGLASSARSAKAQYFAERLAARGLELHCPDFNEP
                     EFETLTCSRMIRQVEAAMAALPPGPVAIIGSSLGGFVAWHVAARQSALRRSRAGTAHP
                     IDRLILLAPAFDFGRTPLGGMDAAGLEAWRRTDRFEIFHHAENRTRFLRYALYEDARQ
                     YDSAACAVEAPTLVFQGLRDAIVDPGMVRRFAAGRPSLTTRFVDDGHQLSASTGIIWR
                     ESAAFLGLIT"
     gene            1993..3189
                     /locus_tag="PLT35_05605"
     CDS             1993..3189
                     /locus_tag="PLT35_05605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOG28738.1"
                     /translation="MTLKLAALAAAAAASMPTCSSAGPLAPIAQASARPPARAAFAPA
                     ASRGGLVYVSAILPPDSSAAFETQATGVFEELKARLDGAGTTIDRVVSATVVLADAGD
                     FPALNAAWNAVWPASRPTRTTIVGRLPVAGARLQVSAIAASAGTSREIVVPPGWQASA
                     LPYSPAIRAGDTLFLSGIVSRRGADNAVVTGDIETQTRTVLDHASAVLQAAGLSLSDV
                     TTARVFLTDVGDFERMNAVYRTYFPSAPPARATVVAQLTSPDYAIEITMVASARRPRS
                     VHVTPNADGSPGRPSPVLSSAIEAGPTLFLSGMLGVAPGADADAAAQAKEALDRLERT
                     MKAAGYGWADVVDSVVYVTDAASAGSVIEVFRQRAGGRLPVGSLVVAGLVSPPARVEI
                     MLTAGR"
     gene            3201..4259
                     /locus_tag="PLT35_05610"
     CDS             3201..4259
                     /locus_tag="PLT35_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOG28739.1"
                     /translation="MPCARPLVGLGVLAAVMLALTAASVGGAAFVAAAAVALLPLPLY
                     LAVALWLDRFEPEPRALLAVAFLWGASAAVVGAGLANGVMEDAIGAVLTSILTAPLVE
                     EALKGAVLAWLFLRRRDEFDGAVDGVIYAAMVGLGFAFAENVDYYGRAFQKGGAGGLA
                     VLFTLRGVLAPFSHPLFTSLTGLGLGVARQSRSRAVSIAAPAAGFAGAVLLHAVWNAG
                     ASAGLVFFGVYALVMLPALAALFASVGFALRFEGRILQAHLAPEAAAGLIGAGDYALL
                     CSVRGRLHALTTALQRGGVADWRRRRAYHRAASELAFLRRRAAIDGVRPDPAAEAAYL
                     QALEAGGRPAGGPAGAGG"
     gene            complement(4274..5506)
                     /locus_tag="PLT35_05615"
     CDS             complement(4274..5506)
                     /locus_tag="PLT35_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOG28740.1"
                     /translation="MTADELLRHVSAFRWHHHATRETMTTAPLADGRVAAVPTFVNEF
                     WTSKQRAAHSLHEISYRACFKPQLPRFFIDRLTEPGAVVYDPFAGRGTTPLEAALAGR
                     TPVACDINPLSAMLLEPRLSPPAVEDVRRRLDAIALGTHVRVRRDLEAFYHPATLREI
                     TALRAYLLERERAGLLDAADRWIRMVTVNRLTGHSAGFLSVYTLPPNQATSVASQLKI
                     NAARGQVPPRRDLRAVVLKKTRALLRDFGEADARALARAAERARIFTRAAHDTPALRG
                     GSVDLVVTSPPFLTIVNYRLDNWLRCWFCGIDPGDVHLTSAHSLAEWQQEMARVFGEL
                     RRVLRPGACAAFEVGEVRGGAVRLEEAAIPAAMDAGLEPVFVLINAQTFTKTANIWGV
                     ANNRFGTNTNRVVLLRRP"
     gene            complement(5664..>6620)
                     /locus_tag="PLT35_05620"
     CDS             complement(5664..>6620)
                     /locus_tag="PLT35_05620"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOG28741.1"
                     /translation="FYHPATLVKDAQRHGVRFAPIDVQHSRWACDVEADGAVRLGLKY
                     VNGLRRQTGTAIEAFGIPSPELRVRFRSLEDFIARTGVNREELATLAEIGALNAFGHH
                     RREALWQIERAVRPAGELFEDGIRDWALDASEAAARRTADQPAAPGVAPPAPGAQRPG
                     PDVPAPGAESLAPGPCPLPPMSLTERLVADYEGTRLTIGPHPMALRRRELSLRGVLRA
                     SDLPLERQGRRVRVAGTVITRQRPGTAKGFVFLTLEDETGISNVIVRPDVFARERLAI
                     LDEPFLLVEGVLQNQDRVVSVRAERVEGIAGVPVEIDSHDFY"
BASE COUNT          880 a         2399 c         2418 g          923 t
ORIGIN      
        1 cagtcgcgcg cgcccgtcca gacgcgcgat ggcgtctcgg tgtggccgcc gcacgagctc
       61 gagtagtaga cgtcggctgg cgccgtcccg gcggtcagaa cgaggccggc ggtcgcgcga
      121 gcggcggcat cggcggcagc ggtcggacgc gcgagcacct ggcagtgcgt caggtcgcac
      181 aggtcgaagc cgtcgttccg gtgccgcccc atgttcgcga gggcgaacgt gcgggccgtg
      241 atcgccatcg cctccagggc ggcgcttggc ccgctccctc ccatctcccc tgttaccacc
      301 tgcgcgacgt agtcctcgag ccgcgtcgtc acgatgtcgt agccgcggcc gtcccggcga
      361 gcgacgccga cgcggaccat gggttcgccg tgcctctggg gcgccccgtg gcggacgagg
      421 atccgggcgg cctcgaccgc ggcatccgaa cctgcccctc ccggcacgag gaccacgatc
      481 gcgtgcaacg gcagctcggt gtcgactgcc gccgtcacga gaccggccgc gccgccgccc
      541 ggcatcggcg ccgtcccggt cttggcgagc cctctcaatc ccgacgccgc caacgccgac
      601 gcggtccccg agcgggccgc cagttcggtg ccagcacgca ggatccgcgc ggcggcggcc
      661 ggcagcgcga cttcgcgcgg tgacagtccg accaggcgca ggaacgcctc cagcagttgc
      721 ggcggccttg cccgcacgcc gcgaagcccg agggccgcgc tcgcggtcgg cgcgtcggga
      781 tcggccggcg gcaggcccag ccggaccagc gccgcgtcca gcgactggcg cgggagccgc
      841 tgggcgacag tcgcgaagaa gacgttgcag gagtgcccca gcgcctccgc ggccgacagc
      901 gggcggtgca ggtccgggtg cacgcacgtc aggcgcttcc cgtcgacgag gatctggcgg
      961 cggcacgaga tgcgggtctg ctcgttgacg atgcccgatt ccaccgccgc gagcagcgtc
     1021 gccagcttca cgatcgatcc gggcgccgtc ggcgccgcca gaaggtccgg gcgcgactcg
     1081 gcgagcacgc ggcccgacgg cagctcgatc aggcggtagg cgggcccgcg gccgccagcc
     1141 gccggcgcct gcgatgaagc gggtgcagcc cggatcatca ccgccgcgca cgcgaccgcc
     1201 gccgccgtca gcacccgccg cgaccgcgtt ccggcgcgca tggcgatcag gtgatcagcc
     1261 cgagaaaggc ggccgactct cgccagatga tgccggtgct ggccgagagc tggtggccgt
     1321 cgtccacgaa ccgagtcgtg agcgaaggcc tgcctgctgc gaaccgccgc accatccccg
     1381 ggtcgacgat ggcgtcgcgc aggccctgga agacgagcgt cggcgcctcc actgcgcagg
     1441 ccgcgctgtc gtactgccgg gcatcctcgt agagcgcgta acgaaggaac cgcgtccggt
     1501 tctcggcatg gtggaagatc tcgaagcggt cggtgcgccg ccacgcctca aggcccgcgg
     1561 cgtccatccc gccgaggggc gtccgcccga aatcaaaggc gggcgcgagc aggatcagcc
     1621 gatcgatcgg gtgcgccgtg cccgcgcggc tccgccgcag ggcggactgg cgggcagcca
     1681 cgtgccaggc cacgaacccg cccaggctcg agccgatgat cgccacggga cccggcggca
     1741 gcgccgccat cgcggcctcg acctgccgga tcatgcgcga gcacgtgagc gtctcgaact
     1801 ccggctcgtt gaagtccggg cagtgcagtt cgaggccgcg ggccgccagg cgctcggcga
     1861 agtactgcgc cttggcagag cgggcggagg aggcgagccc atggaggtag aagacgcgcg
     1921 gccttgtcgc catccggtct aagatacacc ggtcgccggc atgcgcatgc cgccgtatgt
     1981 ggaggatcgc cgatgaccct gaagctggcc gccctcgccg cggccgccgc ggcctcgatg
     2041 cccacgtgct cgagcgccgg tcctctggcg cccatcgccc aggcctccgc tcggccgccg
     2101 gcccgggctg cgttcgcccc cgccgcctcc cgtggcggcc tcgtgtacgt ttccgcgatc
     2161 ctgccgcccg attcgtccgc cgcgttcgag acgcaggcga ctggcgtgtt cgaggagttg
     2221 aaggcccgcc tcgacggcgc cggcacgacg atcgaccgcg tcgtgtcggc gacggtggtg
     2281 ctggcggacg ccggggactt cccggcattg aacgccgcgt ggaacgcggt ctggcccgca
     2341 agccggccga cgcggaccac tatcgtcggc cgcctgccgg tcgctggcgc gcggcttcag
     2401 gtgtccgcca ttgcggcctc cgccggcaca tccagggaga tcgtggttcc gccgggatgg
     2461 caggcgtcgg ccctgcccta cagcccggcc atccgcgcgg gcgacacgct gttcctctcg
     2521 ggcatcgtgt ctcggcgggg cgcggacaac gccgtcgtga ccggcgacat cgaaacgcag
     2581 acgcggaccg tcctcgacca cgcgagcgca gtgctgcagg ccgcagggct ctcgctctcg
     2641 gacgtcacga ccgccagggt cttcctgaca gatgtcggcg acttcgaacg catgaacgcg
     2701 gtctatcgga cgtacttccc gtccgcgcct cccgccaggg cgacagtcgt ggcgcaactg
     2761 acctctccgg actacgccat cgagatcacg atggtcgcct ccgcgcgccg gccgagatcc
     2821 gtgcacgtca cgccgaacgc ggacggctcg ccgggccggc cgagtccggt cttgagttcg
     2881 gccatcgagg ccggcccgac cctgtttctc tcgggcatgc tcggcgtggc cccgggcgcc
     2941 gacgctgatg cggcggccca ggcgaaggaa gcgctcgacc gcctcgaacg gacgatgaag
     3001 gcggcgggct acggctgggc ggacgtcgtg gactcggtgg tatacgtgac cgacgcggcc
     3061 tctgccggat ccgtgatcga ggtcttccgc cagcgggcag gcggccggct gcccgtgggc
     3121 agcctcgtgg tcgccgggct cgtgagccca ccggcgcgcg tggaaatcat gctcacggcg
     3181 gggagataag gcggcgccgc atgccctgcg cgcgccccct cgtcgggctc ggcgtgctcg
     3241 cggcggtgat gctggcgctg acggccgcca gcgtgggtgg cgcggcgttc gtcgcggcag
     3301 ccgccgtcgc gctgctcccg ctgccgctct atctcgccgt cgcgctgtgg ctcgaccgtt
     3361 tcgaaccgga gccccgcgcc ctgctcgccg tcgcattcct ctggggcgcg tccgctgccg
     3421 tcgtcggggc cggtctggcc aacggcgtga tggaggacgc catcggcgcc gtgctcacgt
     3481 cgatcctgac ggcgccgctg gtggaagagg ccctgaaggg cgccgtgctc gcgtggctgt
     3541 tcctgcgcag gcgcgacgag ttcgacggcg cggtggacgg cgtgatctac gccgccatgg
     3601 tcggcctcgg gttcgccttc gccgagaacg tggactacta cggccgggcc ttccagaagg
     3661 gcggcgccgg cggcctggcc gtcctcttca ccctgcgcgg cgtgctggcg ccgttctccc
     3721 atccgctctt caccagcctc accggcctcg ggctgggcgt cgcgcgccag tccaggtcgc
     3781 gcgcggtgtc catcgcggcg cctgcagcgg gcttcgcggg cgcggtcctg ctccatgccg
     3841 tctggaacgc gggcgcctcg gcgggcctcg tcttcttcgg cgtctatgcc ctcgtgatgc
     3901 tgcccgccct ggccgcgctc ttcgcctcgg tggggttcgc cctcagattc gaggggcgga
     3961 tcctccaggc gcacctggcg ccggaggcgg ccgcgggcct catcggcgcg ggcgactacg
     4021 cgctgctgtg ctcggtgcgc ggccggctgc atgcgctcac gaccgccctg cagcgaggcg
     4081 gcgtcgcgga ctggcgaagg cggcgcgcct atcaccgcgc cgcgagcgaa cttgcgttcc
     4141 tccggcgccg ggcggccatc gatggcgtgc ggccggaccc ggcagccgag gcggcgtacc
     4201 tgcaggcgct cgaggccggc ggccgtcccg ccggcggacc cgccggggca ggcggctgag
     4261 ccggcgcgcg gagtcagggc ctgcgaagga gcaccacccg gttcgtgttc gtgccgaagc
     4321 ggttgttagc cacgccccag atgttcgccg tcttcgtgaa cgtctgcgcg ttgatcagca
     4381 cgaacaccgg ttcgaggccc gcgtccatgg ctgccgggat cgcggcctcc tccaggcgca
     4441 ccgcgccccc tctcacttcg ccgacttcga aggcggcgca cgccccaggc ctgagcaccc
     4501 ggcgcagttc gccgaaaacg cgggccatct cctgctgcca ctcggcgagg ctgtgtgcgc
     4561 tggtcagatg gacgtcgccc gggtcgatgc cgcagaacca gcaccgcagc cagttgtcga
     4621 gcctgtagtt gacgatcgtc agaaacggcg gcgaggtgac gaccagatca acggagccgc
     4681 cgcggagggc cggcgtgtcg tgggcggccc gggtgaagat ccgggcccgc tccgccgccc
     4741 tcgcgagggc ccgcgcatcg gcctcgccga agtctctcag cagagcgcgc gtcttcttca
     4801 ggaccacggc ccggaggtcg cgccgcggcg gaacctggcc gcgcgcggca ttgatcttca
     4861 gctgcgaggc gaccgacgtc gcctgattgg gcggcagggt atagaccgac aggaagcccg
     4921 ccgagtgccc cgtcagccgg ttgaccgtca ccatgcggat ccaccgatcc gccgcgtcga
     4981 gcaagcccgc ccgctcgcgt tccagcaggt aggcccgcag ggcggtgatc tcgcgcagcg
     5041 tcgccggatg gtagaaggcc tcaaggtctc gacggacccg gacgtgcgtt ccgagggcga
     5101 tggcatccag acgccggcgc acgtcctcca cggccggcgg cgagagccgc ggttccagca
     5161 gcatggcgct gagcgggttg atgtcgcacg cgaccggcgt gcggcccgcg agtgccgcct
     5221 cgagcggcgt ggtgcctcgt ccggcgaacg ggtcgtagac gacggcgccg ggctcggtga
     5281 gccggtcgat gaagaaccgc ggcagctgcg gcttgaagca cgcgcggtag gagatctcgt
     5341 gcaggctgtg cgcggcgcgc tgcttgctcg tccagaactc gttcacgaag gtcggcaccg
     5401 ccgccacgcg cccgtcggcc agcggcgcgg tggtcatcgt ctcgcgggtg gcatgatggt
     5461 gccacctgaa ggcggacacg tggcggagca gttcgtcggc ggtcatcggg gagatcatag
     5521 tcagaatccg gaagtcagaa gtcagaagca gaatctcccg tggaccggcg ctcacttcag
     5581 tcgaggatgc gccggaatgg agcccgcagg cttcagcatg cgtgagccgg agcgaacgtg
     5641 accgaccggg atgagctccg gggctagtag aagtcgtgcg agtcgatttc gaccggcacg
     5701 cccgcgatgc cctcgacgcg ctcggcgcgc acggacacga cgcggtcctg gttctgcagg
     5761 acgccttcga cgagcaggaa cggctcatcc aggatggcca gccgctcgcg ggcgaagacg
     5821 tccgggcgca cgatgacgtt gctgatgccg gtttcgtcct cgagggtgag gaacacgaac
     5881 cccttggccg tgcccgggcg ctgccgcgtg atgaccgtgc cggccacgcg gacgcggcgg
     5941 ccctggcgct cgagcgggag gtccgaggcg cgcaggacgc cgcgcagcga cagctcgcgc
     6001 cgccgcagcg ccatcgggtg cgggccgatg gtgaggcgcg tgccctcgta gtcggccacg
     6061 aggcgctcgg tcagggacat cggcggcaag gggcaaggac cgggcgccag cgactcggcg
     6121 ccaggcgccg gcacgtcagg gcccgggcgc tgtgcgccag gggctggtgg cgcgacgcca
     6181 ggcgcggcgg gctgatccgc agtgcgacga gctgcggcct cactggcgtc tagcgcccag
     6241 tccctgatcc cgtcttcgaa gagctccccc gccggcctca cggccctctc gatctgccac
     6301 agggcttccc ggcggtggtg gccgaaggcg ttgagcgcgc cgatctcggc cagcgtagcc
     6361 agctcctcgc gattcacgcc cgtgcgggcg atgaagtcct cgagggaacg gaaccggact
     6421 cggagctcgg ggctcgggat tccgaacgcc tcgatcgccg tccccgtctg ccgccgcagc
     6481 ccattcacgt acttcagccc caggcgcacc gcgccgtccg cctcgacatc gcacgcccag
     6541 cgggagtgct gcacgtcgat gggggcgaaa cgcacgccgt gccgctgcgc gtccttcacc
     6601 agcgtggccg ggtggtagaa
//
LOCUS       DAOSIF010000122         6610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10554_length_6610_cov_3.966285, whole genome shotgun sequence.
ACCESSION   DAOSIF010000122 DAOSIF010000000
VERSION     DAOSIF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_6610_cov_3.966285"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..866)
                     /locus_tag="PLT35_05625"
     CDS             complement(228..866)
                     /locus_tag="PLT35_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HOG28742.1"
                     /translation="MSARTRLRVAGVLIAVAVSACGADKPADTAVEYAAGILADRTAK
                     DEAFRRQIDRPIPQARQDAFLPLKYFSIDPQYAVTAVFSPAQTRTLVRMPTSTGLMRD
                     MEMVGTLSFTLKGQALSLGAFVEAGAPPDALFVPFTDLTSGAETYQAGRYLELARNAT
                     GIYIVDFNRAFHPYCYYNPSYDCPYPPPSNRLPVPVRAGEKLAASTIPSAGR"
     gene            complement(863..1588)
                     /locus_tag="PLT35_05630"
     CDS             complement(863..1588)
                     /locus_tag="PLT35_05630"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG28743.1"
                     /translation="MAFEIQGPAGRLEALIDDPACGCRLAPVRPRREFADDPAAGPDD
                     EPRVAVVVAHPLPTAGGTMHNKVVYQAAKGFCRTGAAVLRFNFRGVGTSDGAFDDGPG
                     EMADFRAALDVMAARYPKAELWAAGFSFGAWVAMTVGAEDDRVTALIGIAPPVEQFDF
                     SAVLHSLKPKFFIQGDRDELCPYQATREFYARVPGPKELVTIDGADHLFDGKTSEVAE
                     AVEELLGGYSGLGTRGERGDTSR"
     gene            1796..5200
                     /locus_tag="PLT35_05635"
     CDS             1796..5200
                     /locus_tag="PLT35_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG28744.1"
                     /translation="MSETIQLAEGQILAGRFFPEPVRVETVRLLGSGAWEVGVVEAVS
                     RRFRRVTLTPTDLATIRIQQPVCSFSGDGALLKLGLQAHALGIAYEFDPYFGLSISRV
                     DPLPHQLEAVYDHFLQAARVRFLLADDAGAGKTIMAGLLVRELRLRGLVERVLIVCPA
                     NLTFQWQRELREKFDEKFFVLKGVDIRDQFGINQWIEQDSIITSLDLAKRKEILPGLK
                     QVHWDLVIVDEAHRMSSTSERKTERYRLGELLRDTTDHLLLLTATPHRGDPVNFTMFL
                     RLLDDEAFADVASISEAMKNRHAPFYLRRTKEAMVYFPERQADGTWEAKRIFTKRIPR
                     TAGFAIDGEEDILYRDVTRFVKRQANRAAQRGDDPRARAIGFLMALYQRRLASSTYAM
                     RRSLERRARRLEDALAKAQDLARIAPVDLPDPEELEEMDDADRERLEVMLEAVTLASN
                     PEHVRSEVAELRQLAVQAKSVEDGSLEAKLAKLRAILQQEGFFGQPDQRLLLFTEFKE
                     TLDYLVAKLREWGFKVGFIHGGMRPGSRDEPNSRLWAEQQFREGGFQILVATEAAGEG
                     INLQCCHILFNYDIPWNPNRLEQRMGRIHRYGQTKDCLIFNFVATNTIEGRVLNKLLE
                     KLQEIRDALDDDAVFNVVGEVLPAAHLERVLRDYYAGTLGACDLEERILRDVREDRFR
                     AICQHALEGLATKKLNLAMLVERRARAQERRLVPETTARFIVEAAQAVPMQMKPVKGM
                     DHSFDPGATPLQLKRLEQADSWRLGPIANRYPRLSTDRETADHEKLEWVTPGHPLFEA
                     LRRNALQQASDVLADGACFYSVDSERPARIDVFRAAVVDGLGDTVHERMFAVRQDGSA
                     PSAHDVSILNTLQPAPRPAELPQAAFAPDPLPWLTETALMPFLDEVRCDRVDEVGRIA
                     RHVDVALGELISREDFNIGRYADEAEAGVEGAAGLLRIAEDRHQELIEKRRRRAEALD
                     RQRSMSLQHVERMASLLVLPHPARNDGDVANLRPDPETEQIAMRVVIEHERAQKRIVD
                     DVHLKNLGYDITSVDPASGELRLIEIKGIGADAGTVALTPHERRVAEDRRDCFWLYVV
                     TNCKAEGGPTLLPPIRDPARLAWQEVKKVEYYALSARDLSRSAQTV"
     gene            5242..6321
                     /locus_tag="PLT35_05640"
     CDS             5242..6321
                     /locus_tag="PLT35_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOG28745.1"
                     /translation="MSSGTTGMTFNQFEMLAESRRLEVPVATAWLLAELGESRGRQEP
                     FVRQAPQRLKALREFAVIESAIASNRIEGVEIAPERRDAVLSGRGRLTDRDETEVRGY
                     RDALRLIHQAPSELPVSEDTIRRLHRLVRAGSGDAGAYKQQDSDIIERHPDGRSRVRF
                     RTVPAANTSSAMRSLVGAAERCRDGRWVPPLVTAAAFNLDFLCVHPFRDGNGRVSRLL
                     LLLQAYQAGYEVGRYISLERVIEENKERYYETLEQSSASWHDGRHDPWPYVNFLLFVI
                     RTACAEFERRVGETGEPRGSKTAAVINAIARLPGEFKAADVLRACPAVSLDMVRRVLK
                     DEQRRGRVECLGRGPGARWRKAGRA"
     gene            6365..>6610
                     /locus_tag="PLT35_05645"
     CDS             6365..>6610
                     /locus_tag="PLT35_05645"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOG28746.1"
                     /translation="MISRSCKRLAEVDFPIAVVSKHSAREKSIRHGHPSTLHLWWARR
                     PLAACRAMLMALLLPDPADPDCPAAFKEGARTALRNFP"
BASE COUNT         1123 a         2195 c         2259 g         1033 t
ORIGIN      
        1 agcgcttcgt ccatctcctc cttcgggtgg tcggcgagga tgacgacggc cgacgcgaaa
       61 accctgagcg atcgcgcgcg cttgagcatg gtgactcctc ggtgaacacg cgggcgcatc
      121 gtactatcgc ggggccagaa atcgaaggag tgaaagaccc cggggccgcc tgcgtggaag
      181 ccgccagccg ccgccggccg gcggcccccg gcggcgcggg cgcgcgctca gcggccggcc
      241 gacgggatgg tggacgccgc gagcttctcg ccggcgcgga cggggacggg caggcggttc
      301 gacgggggcg gatacgggca gtcgtaggac gggttgtagt agcagtacgg gtggaacgcg
      361 cggttgaagt cgacgatgta gatgccggtg gcgttccgcg cgagctcgag gtaacgcccg
      421 gcctggtagg tctccgctcc gctcgtcagg tcggtgaacg gcacgaagag cgcgtcgggc
      481 ggcgcgccgg cctcgacgaa cgcgccgagc gacagcgcct ggcccttcag cgtgaaggag
      541 agcgtgccga ccatctccat gtcgcgcatc aggccggtgg aagtcggcat cctgaccagg
      601 gtgcgagtct gcgccggcga gaacaccgcc gtgaccgcgt actgcggatc gatggagaag
      661 tacttcagcg gcaggaacgc gtcctgccgg gcctgcggaa tgggccggtc gatctggcgg
      721 cggaacgcct cgtccttggc ggtccggtcg gccaggatgc ccgccgcgta ctcaaccgct
      781 gtgtccgcgg gcttgtcagc tccgcacgcg gagaccgcca ctgcaatcag gacgcccgcc
      841 acgcgaagtc gtgtccgtgc gctcaccgcg acgtgtcccc gcgttctccc cgagtcccga
      901 gtcccgaata ccctcccagc agctcctcca ccgcctccgc cacctccgac gtcttgccgt
      961 cgaacaggtg gtcggcgccg tcgatcgtca cgagctcctt tggcccgggc acgcgggcgt
     1021 agaactcgcg cgtggcctgg taggggcaca gctcgtcgcg atcgccctgg atgaagaact
     1081 tcggcttgag gctgtgaagg accgccgaga agtcgaactg ctcgaccggc ggcgcaatcc
     1141 cgatgagcgc cgtgacgcgg tcgtcttcgg cgccgaccgt catcgcgacc cacgccccga
     1201 aggagaagcc cgccgcccag agctccgcct tcgggtaacg cgccgccatg acgtccagcg
     1261 cggccctgaa gtccgccatc tcgcccgggc cgtcgtcgaa cgccccgtcg cttgtgccga
     1321 cgccgcggaa gttgaagcgc agcacggcgg ctccggtgcg gcagaagcct ttcgcggcct
     1381 ggtagacgac cttgttgtgc atcgtcccgc cggcggtcgg cagcgggtgg gccacgacga
     1441 cggcgacccg cggctcgtcg tcgggccccg cggccgggtc gtcggcgaat tcccgtctcg
     1501 ggcggacggg cgccagccgg cagccgcacg ccgggtcgtc gatgagcgcc tcgagccgcc
     1561 cggcaggtcc ctggatctcg aacgccacgt gttcagtata cggggtcagg tgagagatcg
     1621 ctcgcgatct tcaatcagcc tccgccgccc tccctagccg ccgtcgttct ggtgtgaccc
     1681 gcgctgtcac ggggtagaac gcgggaccca atcgggtcgt tcttttcggc cgttgaatac
     1741 cgggtggggg ttatcctaga cgcgaccccg acgggactgg gtcgcgaggc aatctgtgtc
     1801 cgagacgatt caactcgcag aaggacagat ccttgcgggg cggttcttcc ccgagccggt
     1861 tcgggtggag accgttcgcc tgctgggcag cggcgcctgg gaagtcggcg tcgtcgaggc
     1921 ggtcagccgc cgttttcgcc gcgtcaccct gacgcccacg gatctcgcga ccatccgcat
     1981 ccagcagccc gtctgttcgt tcagcggcga cggcgcgttg ctgaagctcg gacttcaggc
     2041 gcacgccctc ggcatcgcct acgagttcga cccctatttc ggcctttcca tctcgcgcgt
     2101 ggacccgctt ccgcaccaac tcgaggcggt ctacgatcac ttcctgcagg ccgcccgggt
     2161 ccggttcctg ctcgcggacg atgccggagc cggcaagacc atcatggccg gactgctcgt
     2221 ccgtgaactt agactccggg ggctcgtcga acgcgtcctg atcgtctgcc ccgccaacct
     2281 gacgttccag tggcagcgcg agttacgtga gaagttcgac gagaagttct tcgtcctgaa
     2341 gggtgtcgac atccgagacc agttcggcat caaccagtgg atcgagcagg atagcatcat
     2401 cacgtccctc gacctcgcca agaggaagga gatcctgccc gggctcaagc aggtgcactg
     2461 ggatctcgtc atcgtggacg aggcgcaccg gatgtcgtcg acgtccgagc ggaagaccga
     2521 gcgctaccgc ctgggcgagt tgctccgtga cacgaccgat cacctgctcc tgctgacggc
     2581 cacgccgcac cggggcgacc cggtgaattt cacgatgttc ctgcgcctcc tcgacgacga
     2641 ggcgttcgcc gacgtcgcgt cgatcagtga ggcgatgaag aaccggcacg cgcccttcta
     2701 cctcaggcgc accaaggaag cgatggtcta ctttcctgag cgtcaggcag atggcacgtg
     2761 ggaggcgaag aggatcttca cgaagcgtat cccgcggacc gccggattcg cgatcgacgg
     2821 cgaagaggac atactctacc gggacgtgac gaggttcgtg aagcggcagg cgaaccgcgc
     2881 ggctcagcgg ggagacgatc cgcgcgcccg ggcgattggc ttcctcatgg ccctctacca
     2941 gcgccgcctg gcgtcgagca cctacgccat gcggcggtcg ctcgagcggc gcgcgcggcg
     3001 tctcgaggac gcgttggcca aggcgcagga cctcgcgcga atcgccccgg tcgatctccc
     3061 ggaccctgaa gaactggagg aaatggacga cgccgaccgc gagcgcctcg aggtgatgct
     3121 cgaagccgtc acgctcgcgt ccaaccccga gcatgtgcgc agcgaagtcg cggagctgcg
     3181 gcagctggcc gtccaggcga aatccgttga agacgggagc ctcgaggcca agctggcgaa
     3241 gctccgggcg atacttcagc aggaggggtt cttcggccag ccggaccagc ggctgctgct
     3301 cttcacggag ttcaaggaaa cgctcgacta cctcgttgcg aagctacggg aatggggatt
     3361 caaagtgggc ttcatccacg gcgggatgag gccgggctcc cgcgacgaac ccaactcgcg
     3421 cctgtgggcc gagcagcagt tcagggaggg cgggttccag atcctggtcg cgaccgaggc
     3481 ggcgggcgag ggcatcaacc tccagtgctg ccacatcctg ttcaactacg acatcccgtg
     3541 gaacccgaat cgcctcgagc agcggatggg tcgcatccat cggtacgggc agacgaagga
     3601 ctgcctgatc ttcaacttcg tcgcgacgaa caccatcgag gggcgcgtcc tcaacaagct
     3661 gctcgagaag ttgcaggaga tccgcgatgc gctcgacgac gatgccgtat tcaacgtcgt
     3721 gggagaggtg ctgccggccg cgcacctgga gcgggtgctc cgcgactact acgcgggtac
     3781 gctgggcgcc tgcgacctgg aggagcggat cctccgggat gtccgcgaag atcggttccg
     3841 ggccatctgc cagcacgcgc tggaagggct ggccacgaag aagctgaacc tcgccatgct
     3901 cgtcgagcgg cgggcgcgcg cccaggagcg ccggctcgtc ccggaaacga ccgcgcggtt
     3961 catcgtcgag gccgcccagg cagtcccgat gcagatgaaa ccggtcaagg gcatggatca
     4021 cagcttcgac ccgggcgcca ctcccctcca gctgaagcgc ttggagcagg cggattcgtg
     4081 gcgcctcggt ccgatagcga accgctatcc aaggctgtcg accgatcggg agaccgcgga
     4141 ccacgagaag ctcgagtggg tgacgcccgg gcatcctctg tttgaggcgc tgcgtcggaa
     4201 cgcgctccag caggcgtcgg acgtgctcgc cgacggcgcc tgcttctact cggttgactc
     4261 ggaacggcct gcccgtatcg acgtcttccg cgcggcggtt gtcgacggat tgggcgacac
     4321 cgtgcacgaa cgcatgttcg cagtccggca ggacggatcg gccccgtcgg cccacgacgt
     4381 gtcgatcctc aacaccctgc agcctgcgcc acggcccgct gaactcccgc aagccgcctt
     4441 cgctcccgat ccgctgccgt ggctcacgga aaccgcgctc atgcccttcc tggatgaggt
     4501 ccggtgtgac cgggtagacg aggtgggccg gatcgcccgc cacgtggatg tcgcgctcgg
     4561 tgaactcatc agccgcgagg acttcaacat cggtcgctac gccgatgaag ccgaggccgg
     4621 agtcgagggt gccgccggcc tgctgaggat cgccgaggac cgccaccagg aactcatcga
     4681 gaagcggcgc cggcgggccg aggcactgga ccgccagcgc tcgatgtcgc tccagcacgt
     4741 cgagcggatg gcgagcctgc tcgtcctccc gcaccccgcc cgcaacgacg gcgacgtcgc
     4801 gaacctgcgg ccagacccgg agacagagca gatcgcgatg cgcgtagtga tcgagcacga
     4861 gcgcgcgcag aagcggatcg tcgatgacgt ccacctgaag aacctcgggt acgacatcac
     4921 gagtgtggac ccggcctctg gggaactccg gctcattgag attaaaggga ttggcgcgga
     4981 cgccggcacc gtggcgctta cgccccacga gcgtcgggtc gccgaggatc gccgtgactg
     5041 cttctggctc tacgtcgtca cgaactgcaa ggcggaaggc gggccgacac tgctccctcc
     5101 gatccgcgac ccggcaaggc tggcctggca ggaggtcaag aaggtggagt actacgccct
     5161 ctcggcccgg gacctgagtc gaagcgcaca gaccgtgtag agcacgcctg agactacagg
     5221 aaaggatgta gtgtcccgcc catgagcagc ggaaccaccg gcatgacgtt caaccagttc
     5281 gagatgctgg ccgagtcgcg gcggctcgaa gtgccggtgg ccacggcctg gctgctcgcg
     5341 gagctgggcg agtcgcgggg gaggcaagag ccgttcgtcc gccaggcgcc ccagcgcctg
     5401 aaggccctac gggagttcgc cgtcatcgag agcgcgatcg catcgaaccg gatcgagggc
     5461 gtcgaaatcg cgcctgagcg gcgagacgca gtgctgtccg ggcgcggacg cttgacggat
     5521 cgcgatgaaa cagaagtccg cggctaccgc gacgcgttga ggctgattca tcaggcgccg
     5581 tccgagctgc ccgtctcgga ggacacgatc cgccgcctcc atcggctcgt gcgcgcgggt
     5641 tctggtgacg caggcgccta caagcagcag gacagcgaca tcatcgagcg gcacccggac
     5701 ggccgctcgc gcgtgcgatt ccggacggtg cccgcggcga acacctcatc ggccatgcgg
     5761 tcgctcgttg gggctgccga gcgatgccgt gacggacggt gggtgccgcc gctcgtcact
     5821 gccgccgcgt tcaacctgga cttcctctgc gtgcacccgt tccgggatgg aaatggccgg
     5881 gtgtcgaggc tcctgcttct tctccaggct taccaggctg ggtacgaggt cgggcggtac
     5941 atcagcctgg agcgggtcat agaggagaac aaggagcggt actacgagac ccttgagcag
     6001 tcctcggcct cctggcatga cgggcgccac gatccctggc cgtacgtcaa cttcctcctg
     6061 ttcgtcatca gaaccgcctg cgccgagttc gagcgacggg taggtgagac cggggaaccg
     6121 agaggatcga agacggcggc cgtcatcaat gcaatcgcca ggctcccggg ggaattcaag
     6181 gccgccgacg tcctccgggc gtgccccgcc gtcagcctcg atatggtccg acgggtcctg
     6241 aaagatgagc agaggcgcgg gagggtggag tgcctgggcc gaggacccgg tgcgcgttgg
     6301 cgaaaggccg gccgagcgta attgggtaat atccatataa ataggtaata atttaggcaa
     6361 caacgtgatc tctagatcct gcaagcggtt agccgaggtc gacttcccga tcgcggtcgt
     6421 ctcgaagcac tcggcccggg agaagtccat ccgccacggg cacccgagca cgcttcacct
     6481 gtggtgggca cggaggccgc ttgccgcctg ccgggcgatg ctcatggcgc tccttctgcc
     6541 cgacccggcc gatcccgact gtccggcagc cttcaaggaa ggggcccgga cggccctcag
     6601 gaacttcccg
//
LOCUS       DAOSIF010000123         6531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10732_length_6531_cov_4.159821, whole genome shotgun sequence.
ACCESSION   DAOSIF010000123 DAOSIF010000000
VERSION     DAOSIF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10732_length_6531_cov_4.159821"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLT35_05650"
     CDS             <1..684
                     /locus_tag="PLT35_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG28747.1"
                     /translation="RAIAPLLSLVLLLAALVMAQPDYGSAATLIAIVGVMMFAAGLPL
                     KYLAGLGGLAAGVLAGLAVLEPYRLRRLLAFLDPWQDRFGDGFQVVQSIIAVGTGGVA
                     GRGLGYSVQKLFYLPYPQTDFIFAVLSEELGLVGSTAIVACFAVIAWRGLRTALRAPD
                     AFGSFLALGLTAMIAVQAFVNISVVLGMLPTKGITLPFVSAGGSSLLVSLAGMGVLLN
                     ISQRASATS"
     gene            693..2114
                     /gene="murC"
                     /locus_tag="PLT35_05655"
     CDS             693..2114
                     /gene="murC"
                     /locus_tag="PLT35_05655"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOG28748.1"
                     /translation="MLGRTQRVHIGGIGMSGIAELLSNLGYAVSGTDLKMSEAALRLR
                     AIGVHIALGHDAAHVGDADVVVYSSAVRPDNPEIAEAHRQRIPVIPRAEMLAELMRLR
                     SGIAVAGAHGKTTTTSMVALVLERAGLDPTAVIGGRLSAFGSNARLGRGEYMVAEADE
                     SDRSFLRLAPVVAVITNIDREHLEAYRDFDDLRQAFLEFANKVPFYGAVVACADDPQV
                     RSLVPAMTRRVITYGLAEETADVRGLDVAHEGRRWTCRVVAGAGLARAIGRRGGLASP
                     LCDLRPEIPGWHNLQNALGALAVGLELGLDAGTVAAALGSFRGVDRRFQEIGEAGGVL
                     VIDDYGHHPTEIRAVLDAARASLGRRLVVAFQPHRYTRTGRLLGEFGPALAQADALVL
                     ADVYAAGEEPIPGVTVEALAAAVAPHVGGPIRIARTLDDVIGEVRALARPGDAVITLG
                     AGSIGTISARMLDALAGGAGGRA"
     gene            2116..3000
                     /locus_tag="PLT35_05660"
     CDS             2116..3000
                     /locus_tag="PLT35_05660"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOG28749.1"
                     /translation="MAVTAPADRRFRRSQIHATRRRRLRARPVYRALRAAGVLGLASA
                     GAIWAAGAVARVPWFDVRHIEVSGNQRLHETEVAGLLDGLIGERLLAVDLERWRARLL
                     ASPWVADARLRRRLPATIRVEVRERAPLGLARVAEDLYLVDASGAVIDDYGPRYAELN
                     LPVIDGLVARPASRPPAIDPRRWRLAFRLLAELRTRPELATRVSQVDVSNLHDARVIL
                     EGDPAIVRLGDAQFLERLESYVGLQATLREQVPDIDYVDVRFGERVYVGLSPAPAAAP
                     VQEGTAAAAGRPRARTQR"
     gene            2997..4244
                     /gene="ftsA"
                     /locus_tag="PLT35_05665"
     CDS             2997..4244
                     /gene="ftsA"
                     /locus_tag="PLT35_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOG28750.1"
                     /translation="MSGGVVARRERHLVGLDLGTSKVAAIVGELTGGGEVEIVGLGTA
                     DSEGIRRGAVINQEAAIASIQKAVEAAELTAGVEIDNALVSLTGTHVKGFNSRGVVAV
                     AGRTREITRDDVRRAIDAARAVSLPNGREILHVLPQDFVVDEEDGISNPVGMSGSRLE
                     VNVHIVTGGTTTTQNILSCVNKAGVNVREPVLEQLASSESVLTPDERELGVALVDIGG
                     GTADLAIFERGFLWHTAVMPIGGDHFTNDIAVGLRMPIPEAEKLKRRCGCALSALVGD
                     DETMDVASVGGRAPRTMARRILSDVLQPRAEELCHLVWDEIHRAGYEKSLNSGIVLTG
                     GAANLEGLVQIAEQIFDMPVRRGVPAGLGGLADHIVNPSFATAVGLILRARRQRAHDV
                     NQASRRGIGMLTDLVRGVFKEFF"
     gene            4278..5555
                     /gene="ftsZ"
                     /locus_tag="PLT35_05670"
     CDS             4278..5555
                     /gene="ftsZ"
                     /locus_tag="PLT35_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG28751.1"
                     /translation="MPYSTDIPHTPGARTAASPFRMTMDDTSQTGARIKVIGVGGGGG
                     NAVNRMVREGFDGVQFVVANTDVQALRLNAAPIKVQLGEKLTKGLGAGADPTIGRQAA
                     LEDTEKLVEAIGSADLVFVTAGLGGGTGTGGAPIIASLANQLGALTIAVVTRPFTFEG
                     RKRALQAERGLEELRGCVDSVITIPNERLISTIDRTTSLLDAFAAVDDVLRQAIQGIS
                     DLILLPGLINLDFADVKTIMTGMGVAVMGTGVGRGETRAMDAARKAISSPLLEDASID
                     GARGVIINVTGGADLSLVEVHEASTIIHEAAHEEANIIFGAVIDPRLDGEVKLTVIAT
                     GFDREGARGVVPGSAARTPVDLSAYTAWLGGDPAGARLNLARRHGVGVVAPASPLALT
                     LDAEEGEPAAAAAGGSGADALDVPAFMRRVQQA"
     gene            5668..>6531
                     /locus_tag="PLT35_05675"
     CDS             5668..>6531
                     /locus_tag="PLT35_05675"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28752.1"
                     /translation="MSHAPHRDRTSRGRLREAGVVRKTHGGRLRIALAFPNSYFVGMS
                     NLGFQTAYRLLNQPDDAVCERVFLPQGPDLASGLARPGTLATVESGTKVREFDVLAFS
                     VSFEWDYPNLVRLLRLAGLPVYAAGRDARHPLVIIGGAVTFLNPEPLAPFADVVAVGE
                     GEALVPALVRALARGRDRARALRALAAEPGFYVPGVVDVRYGPDGTIEAFEPRPGSGA
                     TLPIRKAVLSASACADPPATAVFTPDTEFGSRLLVEAVRGCANLCRFCWAGYNYLPVR
                     PFPADRILEIAR"
BASE COUNT          861 a         2351 c         2423 g          896 t
ORIGIN      
        1 agggcaatcg cgccgctcct gagcctggtg ctcctgctgg ccgccctggt catggcgcag
       61 cccgactacg gcagcgccgc gacgctgatc gccatcgtcg gcgtgatgat gttcgccgcg
      121 gggctgccgc tgaagtacct cgcgggcctc ggcgggctgg ccgcgggggt cctcgcgggc
      181 ctcgcggtcc tggagccgta ccgcctgcgc cggctgctgg ccttcctcga cccctggcag
      241 gaccggttcg gcgacgggtt ccaggtcgtg cagtcgatca tcgccgtcgg caccggcggc
      301 gtggcgggcc gcggcctcgg gtacagcgtg cagaagctct tctacctgcc ctacccgcag
      361 acggacttca tcttcgcggt cctgtccgag gaactcgggc tcgtcggctc gacggcgatc
      421 gtcgcgtgct tcgccgtcat cgcgtggcgc gggctgagga ccgccctgcg agcgccggac
      481 gccttcgggt cgttcctggc gctcggcctg accgcgatga tcgcggtgca ggccttcgtc
      541 aacatcagcg tcgtgctggg gatgctgccc accaagggca tcacgctccc gttcgtcagc
      601 gccggcggct cgtcgctcct tgtgagcctg gccggcatgg gcgtgctgct caacatctcg
      661 cagcgcgcgt cggcgacgtc ataggaggag ccgtgctggg ccgcacgcag cgcgtgcaca
      721 tcggcgggat cgggatgagc gggatcgcgg aactcctctc gaacctcggg tacgccgtca
      781 gcggcaccga cctcaagatg tccgaggccg ctctgaggct gcgcgcgatc ggcgtgcaca
      841 tcgcgctggg ccacgacgcg gcgcacgtcg gcgacgccga cgtcgtcgtg tactcctcgg
      901 ccgtgcgccc cgacaatccc gagatcgccg aagcccaccg gcagcggatc ccggtgatcc
      961 cccgggccga gatgctggcc gagctgatgc ggctgcgctc ggggatcgca gtggccggcg
     1021 cccacggcaa gaccaccacc acgtcgatgg tggcgctggt cctcgagcgg gcagggctgg
     1081 acccgacggc cgtcatcggc ggccgcctgt ccgccttcgg cagcaacgcc cggctcggcc
     1141 ggggcgagta catggtggcg gaggccgacg agagcgatcg gtcgttcctg aggctcgcgc
     1201 ccgtggtggc ggtcatcacg aacatcgacc gcgagcacct cgaggcctac cgcgacttcg
     1261 acgacttgcg ccaggcgttc ctcgagttcg cgaacaaggt gccgttctac ggcgccgtcg
     1321 tcgcatgcgc cgacgatccg caggtgcgga gcctcgtgcc ggcgatgacc cggcgggtga
     1381 tcacgtacgg cctcgccgag gagaccgcgg acgtgcgcgg cctcgacgtt gcccacgagg
     1441 ggcgccgctg gacgtgccgg gtcgtggcgg gggccggcct ggcccgcgcc atcggacgcc
     1501 gcggcgggct cgcgtcgccg ctgtgcgatc tgcgccccga gattcccggc tggcacaacc
     1561 tgcagaacgc cctcggggcg ctggccgtcg gcctcgagct cggcctcgac gccggcacgg
     1621 tggcggcggc cctcggctcg ttccgcggcg tcgaccgccg cttccaggag atcggcgaag
     1681 ccgggggcgt gctcgtcatc gacgactacg gccaccaccc gaccgagatc cgggcggtcc
     1741 tggacgctgc gcgggcgtcg ctgggcaggc ggctggtcgt ggccttccag ccgcaccgct
     1801 acacgcgcac cgggcggctc ctcggcgagt tcggtcccgc cctggcgcag gccgacgcgc
     1861 tggtgctggc ggacgtctac gccgccggag aggagccgat ccccggcgtg accgtggagg
     1921 cgctcgccgc ggccgtcgcg ccgcacgtgg gcgggccgat ccgcatcgcg cgcaccctcg
     1981 acgacgtcat cggcgaggtg cgggcgctgg cgcggccggg ggacgcggtg atcacgctcg
     2041 gcgccgggtc gatcggcacg atttcggcga ggatgctcga cgccctggcc ggcggggcgg
     2101 ggggacgggc ctgagatggc agtgaccgct ccggccgatc ggcggttcag acggagccag
     2161 atccacgcca cgcggcggcg gcgcctgcgc gcccggccag tctaccgcgc gctgcgcgcg
     2221 gccggcgtcc tgggcctcgc ctccgcaggg gcgatctggg cggccggggc cgtcgcccgg
     2281 gtgccgtggt tcgacgtccg ccacatcgag gtcagcggga accagcggct gcacgagacc
     2341 gaggtggctg gcctgctcga cgggctgatc ggcgagcgcc tgctcgcggt cgacctcgag
     2401 cgctggcggg cgcggctgct cgcgtcgccc tgggtcgccg acgcgcggct gcggcggcgg
     2461 ctgccggcca cgatccgcgt ggaggtccgt gagcgggcgc ccctgggtct cgctcgcgtc
     2521 gccgaggacc tctatctggt cgacgcgtcg ggcgccgtga tcgacgacta cgggccgcgc
     2581 tacgcggagc tcaacctgcc ggtcatcgac gggctcgtcg cgcgacccgc ctccaggccg
     2641 ccggcaatcg atccgaggcg ctggcggctc gcgttccggc tgctggccga actgaggacg
     2701 cggccggagc tggcgacgcg cgtgtcgcag gtggacgtga gcaacctgca cgacgcccgc
     2761 gtgatcctgg agggcgaccc ggccattgtc cgtctcggcg acgcgcagtt cctggagcgg
     2821 ctggagtcgt acgtggggct gcaggcgacg ttgcgcgagc aggtgccgga catcgactac
     2881 gtggacgtgc gcttcggcga gcgcgtctac gtcgggttgt cgccggcccc ggccgcggcg
     2941 cccgtgcagg aaggcaccgc ggcggcggcc ggcaggccgc gcgcgcggac gcaacggtga
     3001 gcgggggagt cgtggcgcga agggaacggc atctggtcgg gctggacctc gggacctcca
     3061 aggtggcggc catcgtcggc gagctgaccg gcggcggcga ggtcgagatc gtcggcctgg
     3121 gcaccgccga ctcggagggc atccgccgcg gcgcggtgat caaccaggag gcggcgatcg
     3181 cgtccatcca gaaggccgtc gaggcggccg agctgacggc cggcgtcgag atcgacaacg
     3241 ccctcgtgtc gctgacgggc acgcacgtca agggcttcaa cagccggggc gtcgtcgccg
     3301 tggcggggcg cacgcgggag atcacgcgcg acgacgtgcg gcgggccatc gacgccgccc
     3361 gcgccgtgag cctgcccaac ggccgcgaga tcctgcacgt gctcccgcag gacttcgtcg
     3421 tcgacgagga ggacgggatc agcaatcccg tcgggatgag cgggtcgcgc ctcgaggtca
     3481 acgtccacat cgtcaccggc ggcacgacga cgacgcagaa catcctgtcg tgcgtgaaca
     3541 aggcgggcgt gaacgtcagg gagcccgtgc tcgagcagct ggcctccagc gagtccgtgc
     3601 tcacccccga cgagcgggag ctgggcgtcg cgctggtcga catcggcggc ggaacggccg
     3661 acttggcgat cttcgagcgg ggcttcctct ggcacacggc cgtcatgccc atcggcggcg
     3721 accacttcac caacgacatc gccgtcggcc tgcgcatgcc gattccggag gccgagaagc
     3781 tgaagcggcg ctgcgggtgc gcgctgtcgg cgctggtggg cgacgacgaa accatggacg
     3841 tcgccagcgt gggcggccgg gcccccagga cgatggcgcg ccgcattctg tcggacgtcc
     3901 tgcagccgag ggccgaggag ctgtgccacc tcgtctggga cgagatccac cgcgccggct
     3961 acgagaagtc cctcaactcc gggatcgtgc tcacgggcgg cgcggccaac ctcgaaggcc
     4021 tcgtccagat cgccgagcag atcttcgaca tgcccgtgag gcgcggcgtg cccgccggcc
     4081 tgggcgggct cgccgaccac atcgtgaatc cctcgttcgc caccgccgtc ggcctcatcc
     4141 tgcgggccag gcggcagcgg gcgcacgacg tcaaccaggc gtccaggcgg ggcatcggca
     4201 tgctgaccga cctcgtgcgg ggcgtcttca aggaattctt ctaggccgcg atggaatcgc
     4261 cagcagcgga ggccccgatg ccgtactcga ccgacatccc ccacaccccc ggggcgcgga
     4321 ccgccgcgtc gcctttccgg atgacgatgg acgacacgtc gcagaccggc gcccgcatca
     4381 aggtgatcgg cgtcggaggc ggcggcggga acgcggtgaa ccggatggtc cgcgagggct
     4441 tcgacggggt gcagttcgtc gtggccaaca ccgacgtcca ggcgctgcgc ctgaacgcgg
     4501 cgccgatcaa ggtccagctc ggcgagaagc tgacgaaggg cctgggcgcc ggcgccgatc
     4561 cgacaatcgg ccggcaggcc gcgctcgagg ataccgagaa gctggtggag gccatcggca
     4621 gcgccgacct cgtgttcgtg acggcgggac tgggcggcgg caccggcacg ggcggcgcgc
     4681 ccatcatcgc gagcctggcc aaccagcttg gcgccctgac catcgccgtc gtcacgcgcc
     4741 cgttcacctt cgaggggcgc aagcgcgcgc tccaggccga gcgcggcctc gaggagctgc
     4801 gcgggtgcgt ggacagcgtc atcacaatcc cgaacgagcg cttgatcagc accatcgacc
     4861 gcacgacgtc gctgctcgac gccttcgcgg cggtggacga tgtcctgcgg caggccatcc
     4921 agggcatctc ggacctgatt ctcctgccgg ggctcatcaa tctggatttc gccgacgtca
     4981 agacgatcat gacggggatg ggcgtggccg tgatgggcac cggcgtcggg cgcggagaga
     5041 cgcgggcgat ggacgccgcg cgcaaggcga tctcgagccc gctcctcgag gacgcgtcca
     5101 tcgacggcgc gcgcggcgtc atcatcaacg tcaccggcgg agcggacctc tcgctcgtcg
     5161 aggtccacga ggcgtccacg atcatccacg aggccgcgca cgaggaggcg aacatcatct
     5221 tcggcgccgt catcgacccg cggctcgacg gcgaagtgaa gctgacggtg atcgccacgg
     5281 gcttcgaccg ggagggggcc cgcggcgtgg tgcccggcag cgcagcgcgg acgccggtgg
     5341 atctgagcgc ctacaccgcg tggctggggg gggatccggc cggcgcgcgg ctgaacctcg
     5401 cccggcggca cggcgtgggc gtggtcgcgc cagcctcgcc gctcgccctg acgctcgacg
     5461 cggaggaagg ggaacccgcc gcggccgccg cgggcgggag cggcgccgac gcgctcgacg
     5521 tgccggcgtt catgaggcgc gtccagcagg cctagccgag cggaacacgg cgtacaacca
     5581 cggcagcctc gatcgccgcc gccggagcac gagctccagc accgccggcg cccgggcgca
     5641 ggccgcggcg gcgcgccccg aacgcgcatg agccacgcac cacaccggga ccggacatcg
     5701 cgcggacgcc tccgcgaggc cggcgtcgtc cgcaagacgc acggcggccg cctgcgcatc
     5761 gccctcgcgt tccccaacag ctacttcgtc ggcatgtcga acctgggctt ccagacggcg
     5821 taccggctgc tcaaccagcc cgatgacgcc gtctgcgagc gcgtgttcct gccgcaggga
     5881 ccggacctcg cgtccggcct ggcccggccg ggcacgctgg cgactgtcga gtcgggcacg
     5941 aaggtccggg agttcgacgt tctcgccttc tccgtgtcgt tcgagtggga ctatccgaac
     6001 ctcgtgcgcc tgctccgcct cgcgggcctg ccggtatacg ccgccgggcg cgacgcgcgg
     6061 caccccctcg tcatcatcgg cggcgccgtc acgttcctga atcccgaacc gctggccccg
     6121 tttgccgatg tggtcgccgt cggcgaggga gaagcgctcg tgcccgccct cgtgcgcgcg
     6181 ctcgcgcgcg gccgcgaccg agcgcgcgcg ctccgcgcgc tcgcggcgga acccgggttc
     6241 tacgtgcccg gcgtcgtgga cgtccgctac gggcctgacg gcaccatcga ggccttcgag
     6301 ccgcgccccg gatcgggcgc gacgctgccg attcgcaagg ccgtgctgtc cgcctccgcc
     6361 tgtgccgatc cgccggcgac ggccgtcttc acgcccgaca ccgagttcgg ctcgcgcctg
     6421 ctcgtcgagg cggtccgggg ctgcgcgaac ctgtgccggt tctgctgggc gggttacaac
     6481 tacctccctg tgcggccgtt tcccgccgac cgcatcctcg agatcgcgcg g
//
LOCUS       DAOSIF010000124         6512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10767_length_6512_cov_3.982190, whole genome shotgun sequence.
ACCESSION   DAOSIF010000124 DAOSIF010000000
VERSION     DAOSIF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10767_length_6512_cov_3.982190"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLT35_05680"
     CDS             complement(<1..543)
                     /locus_tag="PLT35_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28753.1"
                     /translation="MPAIAVWEDDPLSASPLKPVRRQAPALGAGGLALSFVGKAPEAR
                     VHPVGTPGFRYWNAAEALSRTVRCWGRVLRPDTRWHAGKPLGVDLDAGRDLNAYYDRQ
                     EICFFHETVRGVTVYSGESPDVLCHELGHAILDAVRPQLWNAASIEVASLHESFADIT
                     AMLSALEVDSVRAMVVAATEG"
     gene            complement(650..1735)
                     /gene="queG"
                     /locus_tag="PLT35_05685"
     CDS             complement(650..1735)
                     /gene="queG"
                     /locus_tag="PLT35_05685"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOG28754.1"
                     /translation="MVSAESVKARAQQLGFDLCGVASAGDHPELAFLRQWLDRGFAGR
                     MTSLNRTAAVRADVRRWLPSARSVIVVACSYATAEPDGPTPEDGPRGQVARYARGGDY
                     HEIVGQRLKTMADWLRQEAGPDVSVRWSADTGPVQEKVYARRAGLGWTGKHTLVINPR
                     FGSWIVLGVIATDLALEPDLPIEDRCGTCRRCLEACPTGAIVEPFVLDARRCLACLTI
                     ELRGSIPPDQRADVGRHLFGCDICQEVCPHNAGVALSSRSRWQPRPELEQASLHRLWS
                     AADAELERVIAGTPVSRIGVSGLRRNLAVAIGNSGDPAPLRPSKPADGGSGTHGGAET
                     AAGDPARPSVSDPVVAEHTEWARRRRA"
     gene            complement(1821..4511)
                     /gene="acnA"
                     /locus_tag="PLT35_05690"
     CDS             complement(1821..4511)
                     /gene="acnA"
                     /locus_tag="PLT35_05690"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOG28755.1"
                     /translation="MAHANSFGAVRDLELRGGRLKYCSLPALQQIGFPGVERLPFSLK
                     ILLENLLRHEDGGAVRAGDIQALANWEPGVGIEKEIAFTPSRVLLQDFTGVPAVVDLA
                     AMRDAMAALGGDPNRVNPLQPVELVIDHSVQVDHFGTADAFHLNADIEYARNRERYTL
                     LRWGQSAFANFRVVPPETGIVHQVNLEHLARVVFRAERDGAAWAYPDTVVGTDSHTTM
                     VNGLGVVGWGVGGIEAEAAMLGQPISMLIPTVVGFRLTGALPPGTNATDLVLTVTERL
                     RRHGVVGKFVEFLGPGLANLTLADRATLSNMCPEYGATVAIFPIDAMTIEYLRLTGRD
                     ADTVALIEAYAKAQGLFARAGQPEARYDELVELDLGSIEPSLAGPKRPQDRVPLSAAK
                     AMFSSALPQMLAERKAPPVGRAAAPEPREPAIDHGAVVIAAITSCTNTSNPSVLVAAG
                     LLAKRAVERGLRSKPWVKTSLAPGSKVVTEYLEKAGLTPYLDALGFNLVGYGCTTCIG
                     NSGPLPDAIGAQVEAGGLVVASVLSGNRNFEGRVQPLVRANYLASPPLVVAYALAGSM
                     LVDLTTEPLGIGANGEPVYLKDIWPSERDVQATVAGSVSAGMYRKVYARVFDGDARWQ
                     AIVPPSGDRFTWDDESTYIRNPPYFDRLTLDPPPLRAIAGARVLAVLGDSITTDHISP
                     AGSIKADSPAGRYLQERGVAPRDFNQYGARRGNHEVMVRGTFANVRLKNRLVPGSEGG
                     VTAHLPDGEVMPIYDAAMKYQADGVPLVVLAGKEYGSGSSRDWAAKGTALLGVRAVIA
                     ESFERIHRSNLVNMGLLPLQFEEGASVASLGLTGRETFEVVGAADALVPRGTVTVRAT
                     AEDGSVKAFHARVRIDTPEELVAYRHGGILPYVLRQLLKK"
     gene            complement(4584..5738)
                     /gene="lpxB"
                     /locus_tag="PLT35_05695"
     CDS             complement(4584..5738)
                     /gene="lpxB"
                     /locus_tag="PLT35_05695"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOG28756.1"
                     /translation="MRTLMISCGEPSGDAYAGALARELRRLEPGVRIIGLGGERLRAA
                     GAELIGDYHGLAVTGLVEALAVLPRAWSMKRALVAGARRSRPDALVLVDFPDFNFRLA
                     SSLRPAGVPIVYYVCPQVWAWRRGRLRVMRRLVDRALVIFPFEEAVFGEAGVPAEFVG
                     HPLLDLAPPPGDRRALQEEWGLAPGAPLVALLPGSRPNEVRSTIPTLASAVPLMARRI
                     PGVQFVVGCAPNLDGSLFDPLRRAAGARVALVEGRADDVLRASDAVVTASGTATVQAA
                     IHECPMVIVYRVAPLTYRLGRRVVRLDTFGMANLIAGRRIVPELIQDGFTPEAVTEAA
                     ARYFEDPAHAAATRAALREVRMRLGEPGASRRAAERILALCAARPRRPGS"
     gene            complement(5749..6309)
                     /locus_tag="PLT35_05700"
     CDS             complement(5749..6309)
                     /locus_tag="PLT35_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28757.1"
                     /translation="MTASTAPHAGFAARRTARALAVGALLVAAASAAAGAQRDSEAIG
                     PPRTNFTETALPNGLRIVVHEDHSTPIASAQVWDHAGPANVPSGEHAAAVGACGGQSN
                     ARTTEDVTGHWNTVPAQRLPAVLWMEAGRRVSLRIEPSAFVRERAVQCPCVGVIRRGG
                     DLVPERGLNEVQMNTSFAPSHFAKGE"
BASE COUNT          926 a         2357 c         2370 g          859 t
ORIGIN      
        1 cccctcggtg gccgccacca ccatcgcccg gacggagtcc acctccaggg ccgagagcat
       61 ggcggtgatg tccgcgaacg actcgtgcag cgaggccacc tcgatgctcg ccgcgttcca
      121 cagctgcggc cggaccgcat ccaggatcgc gtgccccagc tcgtggcaga gcacgtccgg
      181 gctctccccc gagtacaccg tgacgccgcg gacggtctcg tgaaagaagc agatttcctg
      241 gcgatcgtaa tacgcgttga ggtcgcgccc ggcgtcgaga tcgacgccca gcggcttgcc
      301 agcgtgccac cgggtgtccg gccgcaggac ccgcccccaa cagcggaccg tccggctcag
      361 ggcctcggcc gcattccagt agcggaatcc cggcgtcccg acgggatgca cacgcgcctc
      421 gggcgccttg ccgacgaagg acagggccag gccgccggcg ccgagggccg gagcctgccg
      481 cctgacgggc ttgagggggg acgcggagag cgggtcgtct tcccagacgg caatggcggg
      541 catgggcata cctccttggc tggccaaggg tagtgactac ccacgcccgt ctcaatggat
      601 cctgcacgcg aatcgagcgc gcgcccgatt cagtgtgcaa cgcaggcggt taggcgcgac
      661 ggcgccttgc ccattcggtg tgttcggcga cgaccgggtc cgacactgag gggcgcgccg
      721 gatcgccagc agccgtttcg gccccgccgt gcgtgccgga accgccgtcg gcaggcttcg
      781 acggccgcag cggggccggg tcgccggaat tgccgatggc caccgcgagg ttcctccgca
      841 agccgctgac gccgatccgg ctcacgggag tgccggcgat gacccgttcc agctccgcgt
      901 ctgccgcgct ccagagccga tggaggctgg cctgctccag ctcgggccgg ggttgccacc
      961 gcgaccggct ggacagcgcc acccccgcat tgtgcgggca cacctcctgg cagatgtcgc
     1021 agccgaagag gtggcgcccg acgtccgcgc gctggtccgg cgggatgctc ccgcgcagtt
     1081 cgatggtgag gcaggcaagg caccgccgag cgtccaggac gaagggctcg acaatcgcgc
     1141 ccgtggggca cgcctcgagg cagcgccggc aggtgccgca ccgatcctcg atgggcaaat
     1201 cgggctcgag ggccaggtcg gtcgcgatca cgccgagcac gatccacgag ccgaaccggg
     1261 gattgatgac cagggtgtgt ttgcccgtcc acccgagccc ggcgcgccgc gcgtagacct
     1321 tctcctggac cggcccggtg tcggcgctcc accggacact gacgtccggc ccggcctcct
     1381 gccggagcca gtcggccatc gtcttcaggc gctgccccac gatctcgtgg tagtcgccgc
     1441 cgcgcgcgta gcgggcgact tgcccgcgag gaccgtcttc gggggtgggc ccgtccggct
     1501 cggccgtcgc gtagctgcag gcgacgacga tgacggacct ggcggagggg agccagcgcc
     1561 ggacatcagc gcggacggcg gcggtgcggt tgagggaggt catcctcccg gcgaaaccgc
     1621 ggtcgagcca ctggcggagg aacgcgagct cgggatggtc gccggccgac gccacgccgc
     1681 acaggtcgaa gccgagttgc tgcgcccgcg ccttcacgga ctctgcagac accatcaatc
     1741 aggagccaga agccagaagt cagaatccgg aattcagaac ccgagtcccc agcccccgag
     1801 tcccgagccc cgagccccgt ttacttcttg agcagctgcc tcagcacgta cggcaggatg
     1861 ccgccgtgcc ggtacgccac cagctcctcc ggcgtgtcga tgcgcacgcg cgcgtggaag
     1921 gccttcacgc tcccatcctc cgcggtcgcg cgcaccgtca ccgtgccgcg cgggacgagg
     1981 gcgtcggccg cgccgacgac ctcgaacgtc tcgcggccgg tgaggccgag cgacgccacc
     2041 gacgcgccct cttcgaactg cagcggcagc agacccatgt tgacgaggtt gctccggtgg
     2101 atccgctcga agctctcggc gatgaccgcc cggacgccga ggagcgccgt gcccttcgcg
     2161 gcccagtcgc gcgatgagcc cgatccgtac tctttgcccg ccaggacgac gagcggcacg
     2221 ccgtccgcct ggtacttcat ggcggcgtcg tagatcggca tcacctcgcc gtccggcagg
     2281 tgcgccgtca cgccgccctc ggagccaggc acgagccggt tcttcaggcg gacgttggcg
     2341 aaggtgccgc gcaccatgac ttcgtggttt ccgcggcggg cgccgtactg gttgaaatcg
     2401 cgcggggcca cgccgcgctc ctgcaggtag cggcctgccg ggctgtcggc cttgatgctg
     2461 ccggccggcg agatgtggtc cgtcgtgatg ctgtcgccga ggaccgcgag cacgcgcgcg
     2521 ccggcgatgg cgcgcagcgg cggcggatcg agggtcagac ggtcgaagta cggagggttc
     2581 cggatgtagg tggactcgtc gtcccacgtg aagcgatcgc cggatggcgg cacgatggcc
     2641 tgccagcggg cgtcgccgtc gaagacgcgc gcgtacacct tgcggtacat cccggcggac
     2701 accgatccgg cgaccgtcgc ctgcacgtcg cgctccgacg gccagatgtc tttcaggtac
     2761 accggctccc cgttcgcgcc gatcccgagg ggctccgtcg tcagatcgac gagcatcgat
     2821 ccggccaggg cgtaggcgac gacgagcggc ggcgacgcga ggtagttggc ccgcacgagc
     2881 ggctggacgc gcccctcgaa gttccggttg ccgctgagca cggaggcgac gacgagcccg
     2941 cccgcctcga cttgcgcgcc gatggcgtcg ggcagcggcc cgctgttgcc gatgcaggtc
     3001 gtgcagccgt acccgaccag gttgaacccg agggcgtcga ggtacggcgt caagccggcc
     3061 ttctcgaggt actcggtgac gaccttcgaa cccggcgcca ggctggtctt gacccacggc
     3121 ttgctcctca gcccgcgctc gacggcgcgc tttgcgagga ggcccgcggc gaccagcacg
     3181 ctggggttcg acgtgttggt gcagctcgtg atggccgcga tgaccacggc gccgtggtcg
     3241 atggcgggct cgcggggctc gggggccgcc gcgcgcccga caggcggcgc cttccgctcg
     3301 gccagcatct gcggcagcgc gctggagaac atcgccttcg ccgccgagag cggcacgcgg
     3361 tcctgcggac gcttcgggcc ggccagcgag ggctcgatcg agccgaggtc cagttcgacc
     3421 agctcgtcgt acctggcctc gggctgcccg gcgcgcgcga aaagcccctg ggccttcgcg
     3481 taggcctcga tgagcgccac ggtgtccgcg tcgcgccccg tcaggcgcag gtactcgatc
     3541 gtcatcgcgt cgattgggaa gatcgccacg gtcgcgccgt actccgggca catgttcgac
     3601 agcgtcgcgc ggtccgccag cgtgaggttc gcgaggccgg ggccgaggaa ctccacgaac
     3661 ttccccacca cgccgtgcct gcgcagccgc tccgtcacgg tgagcacgag atcggtggcg
     3721 ttcgtgcccg gcggcagcgc gccggtcagc cggaagccga cgacggtcgg gatgagcatc
     3781 gagatcggct gccccagcat cgccgcctcg gcctcgatgc cgccgacgcc ccatccgacg
     3841 acgccgagcc cgttgaccat cgtggtgtgg gagtcggtgc ccaccaccgt gtccgggtag
     3901 gcccaggccg ccccgtcccg ctcggcccgg aacacgacgc gcgcgaggtg ctcgaggttc
     3961 acctggtgga cgatcccggt ttccggcggc acgacgcgga agttggcgaa ggcgctctga
     4021 ccccagcgca gaagagtgta gcgctcgcgg ttgcgcgcgt actcgatgtc ggcgttcagg
     4081 tgaaacgcgt cggcggtgcc gaagtgatcg acctgcaccg agtgatcgat gacgagctcc
     4141 accggctgca gggggttcac gcggttcggg tcgccgccga gcgccgccat cgcgtcgcgc
     4201 atggccgcca ggtccacgac ggccgggacg ccggtgaagt cctgcaggag cacgcgcgac
     4261 ggcgtgaacg cgatctcttt ctcgatcccg acgcccggct cccagtttgc gagcgcctgg
     4321 atgtcgccgg ccctgacggc ccctccgtcc tcgtggcgaa ggaggttctc gaggaggatc
     4381 ttcagggaga acggcaggcg ctcgacgccc gggaagccga tctgctggag cgcgggcagg
     4441 ctgcagtact tgagccggcc gccgcggagt tcgaggtccc ggacggcgcc gaaggagttc
     4501 gcgtgggcca tgggttgacc gcctcccggc agtcgaaggc cctattatgc cgcgtttccc
     4561 ggccgcgccc cagagggccc ggctcaggac ccaggccgcc gcgggcgcgc ggcgcacagc
     4621 gcgaggatgc gctcggccgc gcggcggctg gcgcccggct cgccgaggcg catgcggacc
     4681 tcgcgaagcg ccgcccgcgt cgcggcggca tgtgcgggat cctcgaagta ccgggcggcc
     4741 gcctcggtca ccgcctccgg cgtgaacccg tcctggatga gctccggcac gatgcggcgg
     4801 cccgcgatca gattggccat gccgaaggtg tccagacgga cgacgcgcct gccgagccgg
     4861 tacgtgagcg gcgcgacccg gtagacgatc accatcgggc actcgtggat ggccgcctgc
     4921 acggtggccg tgccggaggc cgtcacgacg gcgtcggagg cgcgcagcac atcgtcggcg
     4981 cggccctcga cgagcgccac gcgcgcgccg gcagcgcggc ggagcggatc gaagagggag
     5041 ccgtccaggt tcggcgcgca ccccacgacg aactgcacgc ctgggatccg cctggccatc
     5101 aggggcacgg cggaggcgag cgtcgggatc gtgctccgga cttcgttcgg ccggcttcca
     5161 gggaggagcg ccaccagcgg cgcgcccggc gctaggcccc actcctcctg cagcgcgcgc
     5221 cgatctcccg gcggcggcgc gaggtccagc agcgggtggc ccacgaactc ggctggcacg
     5281 ccggcctcgc cgaagacggc ctcttcgaac gggaagatca ccagcgcccg atcgacgagg
     5341 cgccgcatca cgcggagccg cccgcggcgc cacgcccaca cctgcgggca cacgtagtac
     5401 acgatcggca ccccggccgg ccgcagcgac gacgccaggc ggaagttgaa gtcggggaaa
     5461 tcgacaagga cgagcgcgtc cgggcgagac cgccgcgcgc cggcgaccag cgcgcgcttc
     5521 atcgaccagg cccgcgggag cacggccagc gcctcgacca gccccgtcac cgccagtccg
     5581 tgatagtcgc cgatgagttc cgcgcccgcg gcgcgcagcc gctccccgcc gaggccgatg
     5641 atgcgcacgc cgggctccag gcggcggagt tcccgtgcca gggcgccggc gtaggcatcg
     5701 cccgacggct cgccgcacga gatcatgagg gtgcgcatcg ccgggccgct actcgccctt
     5761 cgcgaagtgc gacggggcga agctggtgtt catctggacc tcgttcaggc cgcgctccgg
     5821 cacgagatcg ccgccgcgcc ggatcacgcc gacgcacggg cactggacgg cgcgctcgcg
     5881 gacgaacgcg ctcggctcga tccggaggct gacccggcgg ccggcttcca tccagaggac
     5941 ggccggcagg cgctgcgccg gcacggtgtt ccagtggccg gtgacgtcct cggtcgtgcg
     6001 ggcgttcgac tgcccgccgc aggcgccgac agccgcggca tgttcgcccg acggcacgtt
     6061 cgccgggccg gcgtggtccc acacctgagc gctcgcgatc ggcgtggagt gatcctcgtg
     6121 gacgacgatc cggaggccgt tcggcagggc cgtctcggtg aagttggtcc tcggcggccc
     6181 gatcgcctcc gagtcgcgct gcgcgccggc agcggcggag gcagcggcga cgaggagcgc
     6241 gccgaccgcc agcgcccggg ccgttctgcg cgcggcgaac ccggcgtgcg gggcggtcga
     6301 ggcggtcatc gcagcgatta tggcacgagc cgcgcgtcag cgccgtccga gggtcacgcg
     6361 gctgacgaag gcctcgaggt gccggaggcc gagccgcccg gccacctccc gcgccagcgc
     6421 cacgcggcgc tcgccgtcca tcgaggccgc gcggcggaga tcggcgagcg gggcgctggg
     6481 ctcgaaccgc cagcggaccc ggcgaaggcc cc
//
LOCUS       DAOSIF010000125         6439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10891_length_6439_cov_4.218515, whole genome shotgun sequence.
ACCESSION   DAOSIF010000125 DAOSIF010000000
VERSION     DAOSIF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10891_length_6439_cov_4.218515"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..900
                     /locus_tag="PLT35_05705"
     CDS             148..900
                     /locus_tag="PLT35_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HOG28758.1"
                     /translation="MADIQRSKASYIFDGLTSGVLPEETAALIRSLIATESLYLAATR
                     EIATKLEVLNDEFKATKDRNPIQFIQTRVKTPKSIVDKLQRRGLPITLESARRHLTDI
                     AGIRVVCSYIDDIYLIAALLVSQDDIALVRFADYIESPKPNGYRSLHLIVTVPVFLSE
                     RTERVAAEIQIRTIAMDFWASLDHELSYKLDEPNRGTTARELRDCADLIAGTDLRMQR
                     LHDIIAGPPQGDPRERWKAEAARAFARAGRSE"
     gene            complement(892..1821)
                     /locus_tag="PLT35_05710"
     CDS             complement(892..1821)
                     /locus_tag="PLT35_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28759.1"
                     /translation="MTGGVSSGPLVRAVASACAALAALWLPGTALAQPGSAAPPRAEI
                     WGAFAVGAPTSGSAIEIAYAPPFRLGGTPLESRANQILAVDTGTAFGLDTGLNLFVSR
                     VFGIQAAFSTRSADIGGANGDFHAFLRYISRPPPDYQPREGTYERSDPWAATAGQLRR
                     RSVALGGVVRWGAGRPVGGTIAGGVEIGRLTGEIESAGYQQFVLGGHSTLFSVRHRAR
                     LRPAAGERLYSPYVGADVHVAMSGRVALAVGVRVPLTPGRSVPVEAAGLVDPSEDAWA
                     PELADVKLALDGQPLELRGTPWHAFAGLKLFLF"
     gene            complement(1927..3099)
                     /locus_tag="PLT35_05715"
     CDS             complement(1927..3099)
                     /locus_tag="PLT35_05715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOG28760.1"
                     /translation="MLDDAPRASRRRFLSQSAAAAAGIALRGLARAQGLPRAEVLETK
                     VISLDTQHYHGWPTAIRRSNGGLLVVCSGGREQHVCPFGRVDLFASHDEGRSWGWPRT
                     LLDGDIDDRDAGILETAKGTLVVTTFTSLAYEPVLARAEADGTWDPARLRRWQAAHAR
                     LGSELRHAELGQWMIRSTDGGRTWSPRYSSIVNSPHGPIQLRDGRLLYAGKELWTGAR
                     RVGVCESLDDGLTWRWLAGIPTRPGDDAANYHELHAAECASGRLVVHLRNQNSANDRE
                     TLQSESGDGGRTWSVPRPIGVWGLPSHLLRLRDGRLLMTYGYRRPPFGNQARISADDG
                     RTWSAPMTISGDGASGDLGYPSTVELADGSLLTVWYEAMAASPMAVLRQATWRLLV"
     gene            complement(3170..4564)
                     /locus_tag="PLT35_05720"
     CDS             complement(3170..4564)
                     /locus_tag="PLT35_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOG28761.1"
                     /translation="MRRTLALAVSAAVLAVGGCSLKPQYVRPASPVPPAFPGTQPAGP
                     DAPQPPDVPWEEYYTDARLRAVVAQALANNRDLRAAMLNIDRAEALYRVQRADRVPSV
                     SAGATVSQQRIPGSVARDGQAYTAAQYTVALGVSGWELDVFGRVRSLTAAALDQYLAT
                     EQAGRAAQLGLVASVAQAWLQRAADADSLALSRATLDAQQAALALIQKSRDLGVASDL
                     ELNQVRSQVEAARADVARYTALVDLDLSALQVLAGAPIDPGLLAGGLSEVSPPRPISA
                     GVSSDVLLRRPDILSAEHQLRAANANIGAARAAFFPRISLTAGLGISSSELSDLVGSG
                     TGTWSFVPQITQPIFNSGAIAAKVRVARVDRELAVARYEKGIQQAFAEVSNTLTLRRT
                     LVSQREAQEALVKALAETSRLSEARYKAGLDGYLGVLVAQRALISAQQALVGVRLAEQ
                     ANLVTLYKVLGGGV"
     gene            complement(4567..>6439)
                     /locus_tag="PLT35_05725"
     CDS             complement(4567..>6439)
                     /locus_tag="PLT35_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG28762.1"
                     /translation="EITSALIGIGLVLAAVFGPMMFFPGSTGVIYRQFSVTIIVSMLL
                     SVVVALVLSPVLCASLLKPVAKGHEAARSGFRPFRPFFLWFDRGFYRGRDAYRGAVAH
                     ILRRKLPYALVFAVIVGGLVVLFSRMPTGYLPDEDQGNLMGIVQLPVGATLEQTEAVL
                     ARARAYFAGNEPDAVESFMGVAGIGMAGRGQNQSMAYIRLKDWDLRRKPGLSAKDVAR
                     RGMGALSRIKEASIYVVPPPPVVELGTATGFDFMLQDRGGLGHEALLRARDQLIRMAA
                     GDPRLARVRPNGMPDVPQYKVDIDWERAGTLGVPVNSIYGYLSAAFGSAYVNDFVQGG
                     RVKRVYAQADAPFRMLPTDLERLHVRNTAGRLVPFSAVASGRWVYGPPRLERYNAVPA
                     MNIQGEPAPGHSSGEAMQAMEELTAKLPAGIAFQWTGLSYQEHMAQSQAGLLYFFSVL
                     VIFLVLAALYESWTVPISILLALPLGVIGSVLASTLRGLPSDVYFQIGLLTVLGLTTK
                     NAILIVQFAQRQQAAGTGLVEATIEGAKLRLRPIVMTSLAFGFGVLPLAIAGGAGAGA
                     QKAIGTSVLGGMLTATFLAIFFIPLFYVFVVRLLGGGAKAEPGRGAAAAAKAAPEVR"
BASE COUNT          983 a         2297 c         2304 g          855 t
ORIGIN      
        1 cgccttgcga ggtcgggcgt ccgctccgga ccacttcggc gcgcgcatcg ccagcgtggc
       61 gcacggggcg cgccctgcgg cgcgcctgcc gcggccggga gccgcgcggt gcgatactgg
      121 gtgcagcccc tgccggaagg agcagccgtg gcggacattc agcgctccaa agccagctac
      181 atcttcgacg gcctgacaag cggcgtgctg cccgaggaga cggccgcgct catccgctct
      241 ctgatcgcga cggaaagcct gtatctggcg gcgacgcgcg agatcgcgac gaagctggag
      301 gtcctgaacg acgagttcaa ggccacgaag gatcggaacc ccatccagtt catccagacc
      361 agggtcaaga cgcccaagag catcgtggac aagctgcagc gccgcggcct tccgatcacg
      421 ctggagtccg cgcggcgtca tctgaccgac atcgcgggca ttcgcgtcgt ctgctcctac
      481 atcgacgaca tctacctgat cgccgccctg ctcgtgtcgc aggatgacat cgcgctcgtc
      541 cgcttcgccg actacatcga gagcccgaag ccgaacggct accgcagcct gcacctcatc
      601 gtcacggtgc ccgtcttcct gtcggagcgc accgagcgcg tcgcggccga aatccagatc
      661 cggacaatcg ccatggactt ctgggcgtcg ctcgatcacg agctgtccta caagctggac
      721 gagccgaacc gcgggacgac ggcgcgggag ctcagggact gcgccgatct catcgccggc
      781 accgacctcc gcatgcagcg cctgcacgac atcattgccg ggcctcccca gggcgacccg
      841 agggagcgct ggaaggccga ggcggcgcgc gccttcgcac gcgccggccg ttcagaatag
      901 gaacagcttc aggccggcga aggcgtgcca gggcgtccct cgcagctcga gcggctggcc
      961 gtcgagggcg agcttcacgt cggccagctc cggcgcccac gcgtcctcgc tcggatcgac
     1021 gaggccggcc gcttcgaccg gcacggaacg gccgggcgtc agcggcacgc ggacgccgac
     1081 ggcgagggcg acgcgcccgg acattgcaac gtggacgtcg gcgccgacgt aggggctgta
     1141 gaggcgctcg cctgccgccg ggcgaaggcg ggcgcggtgc cgcacggaga agagcgtcga
     1201 gtggccgccc aggacgaact gctggtagcc ggcgctctcg atctcgccgg tcaaccgccc
     1261 gatttccacg cccccggcaa tcgtgccgcc aacaggccga cccgcgcccc agcgcacgac
     1321 gcccccgagg gccaccgagc gccgccgcag ctggccggcc gtcgcggccc acggatcgga
     1381 ccgctcgtag gtgccctcac gcggctggta gtcgggcggg ggacgggata tgtaccttag
     1441 gaacgcgtgg aagtcgccgt ttgcgccgcc gatgtccgcc gagcgcgtcg agaacgccgc
     1501 ctggatgccg aagacgcggg acacgaagag gttgaggccg gtgtcgaggc cgaaggccgt
     1561 gcccgtgtcg acggcgagga tctggttcgc gcggctctcg agcggcgtgc ccccaaggcg
     1621 gaagggcggc gcgtaggcga tctcgatcgc gctgccgctt gtcggcgcgc cgacggcgaa
     1681 ggcgccccag atctcggcgc gcggcggagc ggccgagccg ggctgggcca gcgccgttcc
     1741 cgggagccag agggcggcca gcgccgcgca cgcactcgcg accgcccgca cgagcggccc
     1801 gctcgaaacg ccacctgtca tggccgcctt ccctcgatcc cgacaccacg cgtcaggtgc
     1861 ggcgcggacg acgacccgga tgggcgcgcc gccagcgcgc tcgtcatcat cgggatcccg
     1921 agcagtctac accaggagcc tccacgtcgc ctggcgcagg acagccatcg gactggccgc
     1981 catcgcctcg taccacaccg tcagcagcga gccgtctgcg agctcgaccg tgctggggta
     2041 tccgaggtct cccgacgcgc cgtcccccga aatcgtcatc ggggccgacc acgtgcgccc
     2101 gtcgtccgcg ctgatcctgg cttggtttcc gaatggcggg cgccggtagc cgtacgtcat
     2161 gagcagtcgc ccgtcccgca gccgcagcag gtgagacggc aggccccata cgccgatggg
     2221 acgcggcacg ctccacgtgc gtccgccgtc gccggactcc gactgcaggg tctcgcgatc
     2281 gttcgcgctg ttctggtttc ggaggtggac gacgagccgc ccgctggcac actcggcggc
     2341 gtggagctcg tggtaattgg cggcatcgtc gccgggcctg gtcgggattc ccgccagcca
     2401 gcgccacgtc aacccgtcgt cgagcgactc gcacacgccg acccgcctgg cgcccgtcca
     2461 aagctccttg ccggcgtaca gcaggcgtcc gtcgcgaagc tggatgggcc cgtgcggact
     2521 gttgacgatg ctcgagtagc gtggcgacca ggtccggccg ccgtcggttg agcggatcat
     2581 ccactggccc agctccgcgt gccgcagctc ggaaccgaga cgggcgtgcg ccgcctgcca
     2641 gcggcgcagc ctggcggggt cccatgtccc gtcggcctcg gccctggcca ggaccggctc
     2701 gtacgccagc gacgtgaacg tggtcaccac gagcgtgccc ttggccgtct cgaggatccc
     2761 tgcgtcgcgg tcgtcgatgt cgccgtccag cagggtccgc ggccagcccc atgacctccc
     2821 ttcgtcatgg gaggcgaaca gatccacccg cccgaacggg cacacgtgct gctcccggcc
     2881 gccggaacag acgacgagca ggccgccgtt cgagcgccgg atcgcggtcg gccagccgtg
     2941 gtaatgctgc gtgtcgaggc tgatgacttt cgtctccagc acctcggcgc gcggcaggcc
     3001 ctgcgcgcga gcgagcccgc gcagagcgat gccggcggcg gcggcggccg attgcgagag
     3061 gaaccggcgg cgagacgccc gtggcgcatc atcgagcatg ggtatccgtc ctgtgaaccc
     3121 ggccggtgcg ggcccgtttc gcatccggag ggcgcgccgc gcgcgcggct cagacgccgc
     3181 cgcccagcac cttgtagagc gtaacgaggt tcgcctgctc ggccaggcgc acgccgacca
     3241 gcgcctgctg cgccgagatg agcgcccgct gcgcgacgag cacgccgagg tagccgtcga
     3301 ggccggcttt gtagcgcgcc tcggacaggc gggaggtttc cgcaagagcc ttgacgagcg
     3361 cttcctgggc ctcgcgctgg ctcaccagcg tccgccggag cgtcagcgtg ttggagacct
     3421 cggcgaaggc ctgctggatg cccttttcgt agcgcgccac cgcgagttcg cggtcgaccc
     3481 tcgccacccg gaccttcgcg gcgatcgccc cggagttgaa gatgggctgc gtgatctggg
     3541 gaacgaaact ccacgtgccc gtccccgacc cgacgagatc ggacagttcg gaactggaga
     3601 tccccaggcc ggccgtgagc gagatgcgcg ggaagaacgc cgcgcgggcc gcgccgatgt
     3661 tcgcgttggc ggcgcggagc tggtgctcgg ccgacaggat gtccggccgc cgaaggagca
     3721 cgtcggagct cacgccggcc gagatcggcc ggggcgggct gacctcggac aggccgccag
     3781 ccaggaggcc gggatcgatc ggcgctccgg caagcacctg cagcgcgctg aggtcgaggt
     3841 ccaccagggc cgtgtagcgg gcgacgtcgg cccgcgccgc ttccacctgg ctccggacct
     3901 gattcagctc gagatccgag gccacgccga ggtcgcggct cttctggatg agggccagcg
     3961 ccgcctgctg cgcgtcaagg gtcgcccgcg agagcgcgag gctgtccgcg tcagcggccc
     4021 gctggagcca ggcctgggcg actgatgcga ccaggcccag ctgtgccgcg cgccccgcct
     4081 gctcggtggc gagatactgg tcgagcgccg cggccgtcag gctgcggacg cggccgaaga
     4141 cgtccagttc ccatcccgac acaccgagcg ccacggtgta ctgcgccgcg gtgtacgcct
     4201 gcccgtcgcg cgcgacgctg ccgggaatgc gctgctgcga gacggtggcg cccgcgctca
     4261 cagacgggac ccggtcggcg cgctgaacgc ggtacagggc ctccgcccgg tcgatgttga
     4321 gcatcgccgc gcgcagatcc cgattgttgg cgagggcctg cgcgacgacc gccctgaggc
     4381 gtgcgtcggt gtagtactcc tcccagggga cgtccggcgg ctgcggcgcg tcggggccag
     4441 ccggctgggt ccccgggaac gcgggcggca cgggagacgc cgggcggacg tactgcggtt
     4501 tgagcgagca gccgccgacg gccagcaccg cagccgagac ggccaacgcc agtgtgcggc
     4561 gcatggctag cggacctccg gggcggcctt ggcggccgcc gccgcgccgc gccctggttc
     4621 ggccttcgct ccgccgccga gcaggcgaac gacgaagacg tagaacagcg gaatgaagaa
     4681 gatcgccagg aacgtggcgg tgagcatccc gccgaggacg ctcgtgccga tggccttctg
     4741 cgcgcctgcg ccggccccgc ctgcgatggc aagcggcagc acgccgaagc cgaaggccag
     4801 cgacgtcatg acgatcgggc gcaggcgcag cttggcgccc tcgatcgtgg cctcgacgag
     4861 ccccgttccc gcggcctgct ggcgctgggc gaactgcacg atcaggatgg cgttcttggt
     4921 cgtcaggccc agcacggtga gcaggccgat ctggaagtag acgtcgctcg ggaggccgcg
     4981 gagcgtcgag gccaggacgc tgccgatgac gcccagcggc agcgcgagca ggatcgagat
     5041 cggcacggtc cagctctcgt agagggcggc cagcaccagg aagatgacca gcacggagaa
     5101 gaagtacagg agcccggcct gcgactgggc catgtgctcc tggtaggaca ggcccgtcca
     5161 ctggaaggcg atgccggcgg gcagcttcgc ggtgagctcc tccatcgcct gcatcgcctc
     5221 gccggagctg tgccccgggg ccggctcgcc ctggatgttc atggcgggca cggcgttgta
     5281 ccgctccagc ctgggaggac cgtagaccca gcggccggac gccaccgccg agaacggcac
     5341 gaggcgtccg gcggtgttgc gaacgtgcag gcgctcgaga tcggtcggga gcatgcggaa
     5401 cggggcgtcg gcctgcgcgt agacgcgctt cactcgtccg ccctgcacga agtcgttgac
     5461 gtacgcgctg ccgaacgccg ccgacaggta gccgtagatc gagttgacgg gcacgcccag
     5521 cgtcccagcc cgctcccagt cgatgtccac cttgtactgg ggcacgtccg gcatgccgtt
     5581 gggcctcacg cgcgcgagcc gcgggtctcc ggccgccatg cggatgagct ggtcccgcgc
     5641 ccggaggagc gcctcgtggc cgaggccgcc gcgatcctga agcatgaagt cgaagccggt
     5701 cgcggtaccc agctcgacga cgggcggcgg aggcaccacg tagatgctgg cttccttgat
     5761 gcgcgagagc gcgcccatgc cgcgccgcgc cacgtccttc gcgctcagtc cgggcttccg
     5821 cctgagatcc cagtccttga gcctgatgta ggccatcgac tggttctggc cgcgccccgc
     5881 catgccgatg cccgccacgc ccatgaagga ctcgacggcg tccggctcgt tgccggcgaa
     5941 gtaggcccgg gcccgcgcga gcaccgcctc ggtctgctcg agcgtggcgc cgacgggcag
     6001 ctgaacgatt cccatcaggt tgccctgatc ctcgtcgggg aggtagccgg tgggcatccg
     6061 cgagaagagc acgacgaggc ccccgacaat caccgcgaac accagcgcgt agggcagctt
     6121 ccgccgcagg atgtgcgcca cggcccccct gtaggcgtcg cgcccgcggt agaagccgcg
     6181 gtcgaaccag aggaagaacg gccggaaggg gcggaacccg ctccgcgcgg cttcgtggcc
     6241 cttcgccacc ggcttcagca gcgacgcgca cagcacgggc gacaggacga gggccaccac
     6301 cacggacagg agcatcgaca cgatgatcgt caccgagaac tggcggtaga tgacgcccgt
     6361 cgatccgggg aagaacatca tcggcccgaa gaccgccgcc agcacgaggc cgatgccgat
     6421 gagggcgctg gtgatctcg
//
LOCUS       DAOSIF010000126         6437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10899_length_6437_cov_4.953463, whole genome shotgun sequence.
ACCESSION   DAOSIF010000126 DAOSIF010000000
VERSION     DAOSIF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10899_length_6437_cov_4.953463"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /gene="malQ"
                     /locus_tag="PLT35_05730"
     CDS             complement(<1..1208)
                     /gene="malQ"
                     /locus_tag="PLT35_05730"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOG28763.1"
                     /translation="MTPGRAFPAGRRAGLLLPLFSMPSRRSWGLGEFADAALLAPWLA
                     GAGVSALQVLPLNEMAPDQASPYSALSAMALDPIFISVPDVADYQALGGDGSLDGTER
                     RLLDHVRSAARADYRGIRWLKHRALRRAFGRFVEREWKPGTARAAELRAFAEAESSWL
                     DGYAVFRAAHHLLGGESWLDWPAPLRARDRAEIARFAKDAASEVLFRRYLQWIAHEQW
                     RAARARAAPLRVAGDFPFGVAVESADVWADRDLFAFDGSVGAPPDACSATGQNWGLPA
                     YRWDAMRRNGYAWFAARARRAAALFDVFRVDHVVGLYRTWVFPHDGSAPRFVPGDPAG
                     QLEQGEAVMRAIASQGAAVIAEDLGSVPGFVRASLAGLGVPGYRVLRWEREWDEPGRP
                     FIPPAAYPALS"
     gene            complement(1205..2377)
                     /locus_tag="PLT35_05735"
     CDS             complement(1205..2377)
                     /locus_tag="PLT35_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG28764.1"
                     /translation="MFSSRLSFDLRPNRLTAALARLGEARVPVDDLTESNPTRAGLAY
                     PARLLDALGGRAGLEYDPQPFGLMAAREAVAGDYRRRGIQVPRERVALTASTSEAYSL
                     LFKLLCDPGDEVLVPVPSYPLFEHLAGLDAVAPRPYRLEYHGSWALDVDGIRRATTDR
                     TRAVVVVSPNNPTGSYLKRAELDALAAHCGERGLALIGDEVFAEYVLEADLPRTASVL
                     GQRQALAFSLGGLSKGLGLPQLKLGWMAVTGPDDLVRQALERLELVCDTYLSVATPVQ
                     IALPSLLGDGAIVRTQIRDRVARNHQALAEAAAGCPAVGVLRAEGGWHAVLRVPAMQS
                     EESLVLQILEKEHVLVHPGYFYDFPSEAFLVVSLLPRPDVFDRAVSRVIARIEAAA"
     gene            complement(2484..4205)
                     /locus_tag="PLT35_05740"
     CDS             complement(2484..4205)
                     /locus_tag="PLT35_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOG28765.1"
                     /translation="MHPLDWSVIVLYLAWIVWDGVRRSKATSEVEGYFLAKRSLPWWA
                     VGLSVMATQLSAITLIGTTGRGYTDGLGVVQLYLGLPLAMVILSVTAVPFFYRAKVYT
                     AYEYLERRFDARTRTLASLLFLLSRALSCGVIVAAPAVILSIVLGWNLTLTVLGIGLP
                     TVLYTMVGGVQAVTWTDVKQMAVIVAGVGAAVVVLALQLPDRLSVGEALHLAGAAGRM
                     QALDFTWDPNRTYTFWSGLIGGLFLMLSYFGCDQSQVQRYLTARSVDEGRQSLMMSAY
                     VKIPLQLLIPLIGVLVFVFYLFHQPPMLFNRQHEAEVLASARGEEYRALGREFDAAFG
                     ERKAAAEALAAARRTGGAAEAASAASAFRASEARVAGVRARAAGLVREVSGDQAFGDV
                     NYVFPTFVTERMPIGLVGLLIAAIFAAAMSSIAAELNSLSTATVIDFYRRLWRTGESD
                     AHYLRVSKLATLVWGLVACAVALFAADLGPLIDVVNRFGSFFYGSLLGVFVLAIGTRR
                     ATARGAFWGLLAGMAAVALTASYTAIAFLWHNVVGAAVVVAAGMAVSLADRRAGSRRA
                     APVAGRR"
     gene            complement(4604..5095)
                     /locus_tag="PLT35_05745"
     CDS             complement(4604..5095)
                     /locus_tag="PLT35_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG28766.1"
                     /translation="MLSLKTVLVPTDFSEASEAALRYGKAMAEAFGASLHLVHVMEDL
                     LAQAWAAEVYVASMPQLREEIDKESRHRLAAMLTDEERRRYRVETALLAGNPFVEIVR
                     YAKARDVDLIVMGTHGRGPIAHMLLGSVAEKVVRKAPCPVLTVRDAAREASREASRDA
                     AMP"
     gene            5274..>6437
                     /locus_tag="PLT35_05750"
     CDS             5274..>6437
                     /locus_tag="PLT35_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOG28767.1"
                     /translation="MDASGPKALPGAAPDGSAPDGSARLWHVLPNPDVLQALDTSIAA
                     GLASDEARARFERHGPNELAERDRKSAWRSLWEQLSATLIVVLIAAAAISAALGDVTD
                     AVAILAIVALNTALGLVHEQRAEKAMAVLKRMAVPVVRVRRGGAVIELQARELVPGDI
                     VLLEAGNLVPADCRIVECAHLRVEEAALTGESAPIDKVGSALGDAEAAIGDRRNMLYM
                     GTTITYGRGVAAVVATGMATELGRIADLIQAVRRDSTPLQKRLDRLGRVLAGIALVLV
                     AVIFAEGMLTGEDLKTMFLTAVSMAVAAVPEGLPAVVTIALALGAQRMLKRNALIRKL
                     PAVEALGSVTVICSDKTGTLTENRMTVTVLDVAGERLDVVEALHAAGPSVDIEP"
BASE COUNT          947 a         2431 c         2323 g          736 t
ORIGIN      
        1 gacagcgccg ggtacgccgc cggcgggatg aacggccggc ccggctcgtc ccactcgcgc
       61 tcccagcgca gcacgcggta gccgggcacg ccgagcccgg cgagggaggc gcgcacgaac
      121 ccgggcacgc tgccgaggtc ctcggcgatg acggcggcgc cctgggacgc gatggcgcgc
      181 atcacggcct cgccctgctc gagctgcccg gcgggatcgc cgggcacgaa gcgcggggcg
      241 ctgccgtcgt gcgggaagac ccacgtccgg tagagcccga cgacgtgatc gacgcggaag
      301 acatcgaaga gggcggccgc gcgccgggcg cgcgccgcga accacgcgta gccgttccgg
      361 cgcatggcgt cccagcggta cgccggcagg ccccaattct ggcccgtcgc gctgcaggcg
      421 tccggcgggg cgccgacgga gccgtcgaag gcgaacaggt cccgatcggc ccagacgtcg
      481 gcgctctcga cggcgacgcc gaagggaaag tcgcctgcga cgcgcagcgg cgccgcgcgc
      541 gcccgcgccg cgcgccactg ctcgtgcgcg atccactgga ggtagcgccg gaagagcacc
      601 tccgaggcgg cgtccttcgc gaagcgcgcg atttcggcgc ggtcgcgcgc gcgcagcggc
      661 gcgggccagt cgagccagga ctcgccgccc agcaggtggt gcgcggcgcg aaacacggcg
      721 tagccgtcga gccacgacga ctcggcctcg gcaaacgcgc gcagctcggc cgcgcgcgcc
      781 gtgccgggct tccactcgcg ctcgacgaac cgcccgaacg cgcgccgcag cgcccggtgc
      841 ttcagccacc tgatgccgcg gtagtccgcg cgcgccgcgc tgcggacgtg atccagcagc
      901 ctgcgctcgg tgccgtcgag cgagccgtcg ccgccgagcg cctggtagtc ggccacgtcg
      961 ggcaccgaga tgaagatggg gtcgagggcc atcgcgctga gcgccgagta gggcgacgcc
     1021 tggtccgggg ccatctcgtt gagcggcagc acctgcagcg cgctcacgcc cgcgcccgcc
     1081 agccacggcg caagcagcgc cgcgtcggcg aactcgccga ggccccagct tcggcgcgac
     1141 ggcatcgaga agagcggcag cagcaggccg gcgcggcggc ccgccgggaa cgcccggccc
     1201 ggcgtcacgc ggcagcctcg atgcgggcga tcactcggct gacggcgcgg tcgaacacgt
     1261 cggggcgcgg caggaggctg acgacgagga aggcctcgct cggaaaatcg tagaagtacc
     1321 cggggtgcac gagcacgtgc tccttctcga ggatctgcag gaccagcgac tcctccgact
     1381 gcatggccgg cacccgcagc acggcgtgcc agccgccctc ggcccgcagc acgccgaccg
     1441 ccgggcaccc ggcggccgcc tcggcgaggg cctgatggtt gcgcgcgacc cggtcccgga
     1501 tctgcgtgcg cacgatcgcg ccgtcgccga gcagcgacgg cagcgcgatc tggacgggcg
     1561 tggccaccga caagtaggtg tcgcagacga gctcgaggcg ctccagcgcc tgcctgacca
     1621 ggtcgtccgg gcccgtcacg gccatccacc cgagtttcag ctgcggcagg ccgaggccct
     1681 tcgacagccc gccgaggctg aaggcgagcg cctggcgctg gcccagcacg gacgccgtcc
     1741 tcggcaggtc cgcctcgagc acgtactccg cgaacacctc gtccccgatg agggcgaggc
     1801 cacgctcgcc gcagtgcgcc gccagcgcgt ccagctcggc gcgcttcagg tacgagcccg
     1861 tcgggttgtt cgggctgacc accaccaccg cccgcgtccg gtcggtcgtc gcgcgcctga
     1921 tgccgtcgac gtcaagggcc cagcttccgt ggtactcgag gcggtagggc cgcggcgcca
     1981 cggcgtcgag cccggccagg tgctcgaaga gcgggtagct cggcaccggc accagcacct
     2041 cgtcgcccgg gtcgcagagc agcttgaaga gcagcgagta cgcctcgctc gtgctcgccg
     2101 tgagcgccac gcgctcgcgc ggcacctgga tgccgcggcg gcggtagtcg cccgccacgg
     2161 cctcgcgcgc ggccatcagg ccgaagggct gcgggtcgta ctccaggccc gcgcgcccgc
     2221 cgagggcatc gagcagccgc gcggggtacg caaggcccgc gcgcgtgggg ttcgactcgg
     2281 tgagatcgtc aaccggcacg cgggcctcgc caaggcgcgc cagggccgcc gtcagccggt
     2341 tggggcggag atcgaaggac aggcgtgatg agaacactga acgaacagga taccgcagct
     2401 tcgcccgcgg atggggcggc gcggcgcccg ccagcgcaag gccgccaacg ccgcgccggc
     2461 cgccgcgcgc gatcggcccg ggcctaccgc cgcccggcaa ccggggccgc gcggcgcgag
     2521 ccggcgcgcc gatcggccag actgacggcc atgcccgccg ccaccaccac cgccgcgccc
     2581 accacgttgt gccagagaaa cgcgatggcc gtatagctcg ccgtcagcgc cacggcggcc
     2641 atcccggcca gcaggcccca gaaggcgccg cgcgccgtcg cgcgccgcgt cccgatggcg
     2701 aggacgaaca cgccgagcag cgacccgtag aagaacgacc cgaagcggtt cacgacgtcg
     2761 atgagcgggc cgaggtccgc cgcgaagagc gccaccgcgc acgcgacgag gccccagacg
     2821 agcgtcgcga gcttcgacac ccgcaggtag tgcgcgtccg actccccggt ccgccacagc
     2881 cggcggtaga agtcgatgac ggtcgcggtc gagagcgagt tcagctcggc ggcgatgctc
     2941 gacatggccg cggcgaagat ggccgcgatg agcaggccga cgagcccgat cggcatgcgc
     3001 tcggtgacga aggtcgggaa cacgtagttc acgtcgccga acgcctggtc gccgctgacc
     3061 tcccgcacga ggcccgccgc gcgcgcccgc acgccggcca cgcgcgcctc gctcgcccgg
     3121 aaggccgacg ccgccgatgc ggcctcggcc gcgccgcccg tccgccgcgc cgccgccagc
     3181 gcctcggccg ccgccttccg ctccccgaac gccgcgtcga actcgcgccc gagggcgcgg
     3241 tactcctcgc cgcgcgcgct ggcgagcacc tccgcctcgt gctggcggtt gaacagcatc
     3301 ggcggctggt ggaacaggta gaagacgaag acgagcacgc cgatgagcgg gatgaggagc
     3361 tggaggggga tcttgacgta cgcgctcatc atgagcgact gccggccctc gtcgacggac
     3421 ctggcggtga ggtagcgctg cacctggctc tgatcgcagc cgaagtacga cagcatcagg
     3481 aacaacccgc cgatgagccc cgaccagaac gtgtaggtcc ggttcggatc ccacgtgaag
     3541 tcgagggcct gcatgcgccc cgccgcgccc gccaggtgca gcgcctcccc caccgacagg
     3601 cgatccggca gctgcagcgc cagcacgacg acggccgccc cgacgcccgc gacgatgacc
     3661 gccatctgct tcacgtcggt ccacgtcacc gcctgcacgc cgccgaccat cgtgtagagc
     3721 accgtcggca ggccgatgcc caggacggtc agcgtcaggt tccagccgag cacgatcgac
     3781 aggatcaccg ccggcgccgc cacgatcacg ccgcacgaca gcgcgcgcga cagcaggaag
     3841 agcaggctcg ccagcgtccg cgtccgcgcg tcgaagcgcc gctcgaggta ctcgtaggcg
     3901 gtgtagacct tcgcgcggta gaagaacggc accgccgtca ccgacaggat caccatcgcg
     3961 agcggcaggc cgaggtagag ctgcacgacg ccgaggccgt cggtgtagcc gcgcccggtc
     4021 gtgccaatca gcgtgatggc cgagagctgc gtcgccatca ccgacagccc caccgcccac
     4081 cacggcagcg accgcttcgc gaggaagtag ccctcgacct cgctcgtggc cttcgagcgg
     4141 cgcacgccgt cccagacgat ccacgcgagg tagaggacga tgacggacca gtcgagcggg
     4201 tgcatgggag acaggcgcta ggggccgggg gcggggcgct gggcgctggt cagttccgat
     4261 gtcacgctgt gcggcctccc acgcggcgcg agccgcatcc ggcgagcccc tggcgccagg
     4321 tccttgatcc ctggcggtga cccgcctccg ggcgggcggg ccgccccgcg cgggcggccg
     4381 gcgatcacgc gctgaagtac cgctggatga gccagattgc ggccagcacg atggcctcga
     4441 gggcgatcgc gccggcgtag agcaggcggc tgttcgatct cggggatgcc atgggctcct
     4501 gtcgatccgg gccatcgcca ggggaagggc gcccgcgcgc caggcgccgg cccgcggatg
     4561 ccgccgggct cggccgcccg ccccctggcc gccggctctt cctctacggc atcgccgcgt
     4621 cgcgcgatgc ctcgcgcgat gcctcgcgcg ccgcgtcccg caccgtcagc acggggcacg
     4681 gcgccttccg caccaccttc tccgccacgc tcccgagcag catgtgcgca atcgggccgc
     4741 gcccgtgcgt ccccatcacg atgagatcca cgtcgcgggc cttggcgtag cggacgatct
     4801 cgacaaacgg gttgcccgcc aggagcgccg tttccacgcg gtagcggcgg cgctcctcgt
     4861 cggtcagcat cgccgccagg cggtgcctcg attccttgtc gatctcctcg cgcagctgcg
     4921 gcatcgacgc cacgtagacc tcggccgccc acgcctgcgc cagcagatcc tccatcacgt
     4981 gcacgaggtg cagcgacgcg ccgaacgcct cggccatcgc cttgccgtag cgcagggccg
     5041 cctcggacgc ctcgctgaag tcggtgggga cgaggacggt cttgagggag agcatggcac
     5101 ctcctcgtga acgggcgccg ggggccaggc gcagggcgct ggtggtgatc cgctgggtgg
     5161 attatacggc cgatgcgccg aaacggcgcg ccccgggcgg gccgcggctc ggcgcggcaa
     5221 gccgcggagt tgcccctggc ccctgcacgc gcgacaattg gactggagac acgatggacg
     5281 catccggccc gaaggccctg cccggggcgg cccccgacgg atcggccccc gacggatcgg
     5341 cgcggctctg gcacgttctg ccaaaccccg acgtcctgca ggcgctcgac acgagcatcg
     5401 ccgccggcct ggcgagcgac gaggcccgcg cgcgcttcga gcggcacggc cccaacgagc
     5461 tcgcggagcg ggaccgcaag agcgcctggc gctcgctgtg ggagcagctg agcgccacgc
     5521 tcatcgtcgt gctgatcgcg gccgcggcca tctcagccgc cctgggcgac gtgaccgacg
     5581 ccgtcgccat cctcgccatc gtggcgctca acaccgcgct ggggctcgtc cacgagcagc
     5641 gcgccgagaa ggccatggcg gtcctcaagc gcatggccgt gcccgtcgtg cgcgtgcggc
     5701 gcggcggggc ggtgatcgag ctgcaggccc gcgaactcgt gccgggcgac atcgtcctgc
     5761 tcgaggccgg caacctcgtg cccgccgact gccgcatcgt cgagtgcgcg cacctgcgcg
     5821 tggaggaggc cgcgctgacg ggcgagtcgg cgcccatcga caaggtcggc agcgccctcg
     5881 gcgacgcgga ggccgccatc ggcgaccgcc ggaacatgct ctacatgggc acgacgatca
     5941 cgtacgggcg cggcgtcgcc gccgtcgtgg ccaccggcat ggccaccgag ctcggccgca
     6001 tcgccgatct catccaggcc gtccggcgcg acagcacgcc gctccagaag cgcctcgacc
     6061 gcctcgggcg cgtcctcgcg ggcatcgccc tcgtcctggt cgccgtcatc ttcgccgagg
     6121 ggatgctgac gggcgaggac ctgaagacga tgttcctcac ggccgtgagc atggccgtcg
     6181 cggccgtgcc cgagggcctg ccggccgtcg tgaccatcgc cctggccctc ggggcgcagc
     6241 ggatgctgaa gcgcaacgcc ctcatccgca agctgccggc cgtcgaggcg ctcggctcgg
     6301 tgacggtgat ctgctcggac aagaccggca cgctcaccga gaaccgcatg accgtcacgg
     6361 tgctcgacgt cgccggcgag cggctggacg tcgtcgaggc gctgcacgcc gccgggccgt
     6421 cggtggacat cgagccg
//
LOCUS       DAOSIF010000127         6410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_10954_length_6410_cov_3.693784, whole genome shotgun sequence.
ACCESSION   DAOSIF010000127 DAOSIF010000000
VERSION     DAOSIF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10954_length_6410_cov_3.693784"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLT35_05755"
     CDS             complement(<1..1239)
                     /locus_tag="PLT35_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG28768.1"
                     /translation="MNDARSLPPWAEDLRRRYRRGEASMFVLHGNVYDAVVHGRDTSS
                     LVDFLTDVLLRDTRETIAVYNMATGPRFSKRAKDARGADEVLGDTHKDKVLPAFERLL
                     VGSMRAAVILEYAEALAPAGDPAFQADADRAAIIMLHRWSSLPAIEKGDNIVLLVAEN
                     LSDLAAKIVSNPKVAVVEVPMPDRAARRAVARIANPGLTEQDADRYAEVTAGLKAVQI
                     ASILSPPPASEAEAAQREAFIAGILGPGPDVPPRAKTLAAMTAGMSQEDIRRLIAPGA
                     PASGPDTAAADRARQDVDRLIARRKREILERECFGLVEFVEPEHGFDVVGGMEEVKKD
                     LRVVAENIRDGRASRVPMGILFTGPMGTGKTFVAEAFAKECGLTTIKLKNFRSKWVGA
                     AIRRSSRRWRGAGTGGCGPGR"
     gene            complement(1246..2163)
                     /locus_tag="PLT35_05760"
     CDS             complement(1246..2163)
                     /locus_tag="PLT35_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28769.1"
                     /translation="MAAPERPGPRAADPAPPSRSAAARDRELPPVVEAVAASARDALI
                     EKRPYLKYAFTNPYNLSLFLGALAAAGLTLNPLLAVVAAGAEALWLLYAPESARLRHL
                     LWDPRFEKVKEALEREARAERMTWLSQGDRARVDRLVTQKRDINRLASQNPSFTAELL
                     QNELVKTDRLVEAFLDMAVTCARYEQYLGSIDVAALDKDRERWDRAIRAGKDGEAQTE
                     IAKKNLAIIMKRFEKVQEIRKYLGVARGQLDLIENSFRLLADQIVTMQSPQELSGQLD
                     ELLDGVQTIKDSAADTERMLSSLGLASNA"
     gene            complement(2173..3072)
                     /locus_tag="PLT35_05765"
     CDS             complement(2173..3072)
                     /locus_tag="PLT35_05765"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28770.1"
                     /translation="MSGQTRGAEPGEPGSAAPAVDGRTAGAPPTYRADLAAKPPKLRV
                     KGLGVTYIGRDGHETEAVRDVSFDIEDKPGAGEIVVFLGPSGCGKSTILKAVAGLIPP
                     SAGDILVDGHPVTGPGRDRGMVFQAYTSFGWLTVRDNVEYGLRLQGMPASERRALSDQ
                     YLASVGLTEFANRFPKDLSGGMKQRVAIARTLINQPRVILMDEPFGALDPQTRWGMQG
                     LLLDVSRTMDNTILFVTHDVSEAVYLADTIYVLSARPARILHQVETPFFQNRDMALKS
                     APEFRAVEKRLLDMLYAPGAATA"
     gene            complement(3069..3872)
                     /locus_tag="PLT35_05770"
     CDS             complement(3069..3872)
                     /locus_tag="PLT35_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG28771.1"
                     /translation="MARRTSPLFALRTPLPRSTSLILGLVMPAVVLGAWSAASYGGLA
                     PPDFLPSPSEVVRGTLQLFLQHNLASAILISTKRIVFAFLLASAIALPLGVLMGAFEP
                     VNRLFEPIMAPLRYMPISAFIPLLILWFGIYEKQKIAFLFLGVFVYLLPVVVTAIRAV
                     AEELVQTGLTLGAGRWQVVRTILVPAALPEIFDSFRVMNAISWTYVILAEAVNPEHGL
                     GYMVELARTHQKASWSFAGLLVIGGIGLLTDGLIRTASSMLFRWREQTA"
     gene            complement(3877..5511)
                     /locus_tag="PLT35_05775"
     CDS             complement(3877..5511)
                     /locus_tag="PLT35_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HOG28772.1"
                     /translation="MKLTPFAKLFITIVILAVVGYAAYHYKGAQIRQWAEGGKGAGPQ
                     PAQQVDRDDFSALGGMQADPARTAGSTGVTRTALARSGRLGRPLVVGINTWAGHAPGV
                     VFNAGLDANPASNYRKRFGFDVKFVLLEDPAAKLAAFRNGDIDVMWNTVDNWAREASI
                     LAEQNQQAKSIIMQDWSRGGDGIVSLASITSIEGLKGRKIACTQFTPSHFLLLYLLAQ
                     SGLSAEDRAAVEKRIIFTQDAPAAAAMFKAKQVDAAVTWEPDLSGAVAAREGEAHVLV
                     STTAATNIIADTLCARQDLIDRAPETLRDFVHGWFEGIELMKADPASSYAVVGRALKL
                     DNETVSGMLSGLKLTPYADNAQFYGLTGGKAHYETLFDTAFVIWRKKGLVTRPVDARD
                     WADTRFISALAEAYPGQTVQEPAIAARKASTSDRAIINKQIQIHFTPGSDVIMEGSYF
                     VLDALGETMTSFGNTYLRIEGNTDSVGSAAANLTLSERRALAVKNYVLKHFPNIQPAR
                     FQTIGRGEENPVASNETEAGRQLNRRTDIKVILATQ"
     gene            complement(5573..6283)
                     /locus_tag="PLT35_05780"
     CDS             complement(5573..6283)
                     /locus_tag="PLT35_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOG28773.1"
                     /translation="MAGGFFARLGNLWRGFLSLWISDVEKQHPEIAYENAINSMIAKY
                     TKLKTATAAIIRRREDIDQRNQKAKAELAEVDGQLAAALDTNQDDLAVVLLQKKNELE
                     KELAELQADVQLAQKDADSAKNSLLTVQAEIKKLKAERDSMLAKMHSAQARLKIQEQL
                     DGLSVDAEVKALDNVREHIRTTIAEANLGRELSESSLDARLDALRNQAGEVQARARLA
                     ELKAKRAAQQAAQGQKTL"
BASE COUNT          907 a         2235 c         2219 g         1049 t
ORIGIN      
        1 gcgcccaggc ccgcagccac ccgtacccgc gccgcgccac cgcctgctcg agcgtcgaat
       61 agccgcgccc acccatttcg accggaagtt cttcagcttg atcgtcgtca ggccgcactc
      121 tttcgcgaag gcctcggcga cgaacgtctt gcccgtgccc atcggcccgg tgaagaggat
      181 gcccatgggg acgcggctgg cgcggccgtc gcggatgttc tcggcgacga cgcgaagatc
      241 cttcttcacc tcctccatcc cgccgacgac gtcgaatccg tgctcgggct cgacgaactc
      301 gacgaggccg aagcactcgc gctcgaggat ctcgcgcttg cgccgcgcga tcaggcgatc
      361 cacgtcctgc cgcgcccggt ccgccgccgc cgtgtcggga cccgacgccg gggcgcctgg
      421 cgcgataagg cgtcggatgt cctcctgcga catgcccgcc gtcatcgcgg cgagcgtctt
      481 cgcccgcggc ggtacgtccg gaccggggcc gaggatgccg gcgatgaacg cctcgcgctg
      541 ggcggcttcg gcctccgacg ccggcggcgg cgagaggatc gaggcgatct gcaccgcttt
      601 caggccggcg gtcacctccg cgtaccgatc ggcgtcctgc tcggtgagcc cggggttggc
      661 gatcctcgcc acggcccgcc gcgccgcccg gtcgggcatg ggaacctcca cgacggccac
      721 cttcgggttc gacacgatct tcgccgccag gtcgctcagg ttctccgcga cgagcaggac
      781 gatgttgtcg cccttctcga tggccgggag cgacgaccag cggtggagca tgatgatggc
      841 cgcccggtcc gcgtccgcct ggaaggccgg atcgccggcc ggcgccagcg cttccgcgta
      901 ctcgaggatg actgccgcgc gcatcgatcc gacgagcagg cgctcgaagg ccggcagcac
      961 cttgtccttg tgcgtgtcgc ccagcacctc gtctgcgccg cgcgcgtcct tcgcccgctt
     1021 cgagaagcgc gggccggtcg ccatgttgta gacggcaatc gtctcgcggg tgtcccggag
     1081 gaggacgtcc gtcaggaagt ccacgaggct cgaggtgtcg cggccgtgca cgaccgcgtc
     1141 gtacacgttg ccgtggagga cgaacatcga ggcctcgccg cgccggtagc gccggcggag
     1201 gtcctcggcc cagggcggga ggcttcgcgc gtcgttcatg gtgcgtcagg cgttgctggc
     1261 caggccgagg ctgctgagca tgcgctcggt gtcagcggcg ctgtccttga tggtctgcac
     1321 gccgtccagg agctcgtcga gctggcccga cagctcctgc ggcgactgca tcgtgacgat
     1381 ttgatcggcg agcagccgga acgagttctc gatgagatcg agctgcccgc gcgcgacgcc
     1441 gaggtacttg cggatctcct gaaccttctc gaatcgcttc atgatgatcg cgagattctt
     1501 cttcgcgatc tccgtctgcg cttcgccgtc cttgcccgcc cggattgccc gatcccagcg
     1561 ttcgcggtcc ttgtcgagcg ccgcgacgtc gatcgagccg aggtactgct cgtagcgggc
     1621 gcaggtgacc gccatgtcca ggaacgcctc gacgagccga tcggtcttga cgagctcgtt
     1681 ctggagcagt tcggccgtga aggacgggtt ctgcgacgcg aggcggttga tgtcgcgctt
     1741 ctgcgtcacg aggcgatcca cgcgggcgcg gtccccctgc gaaagccacg tcatccgctc
     1801 ggcccgcgcc tctcgctcga gcgcctcctt caccttctcg aaccgcggat cccacaggag
     1861 gtgccgcagg cgcgccgact ccggcgcgta cagcagccac agcgcctcgg cgccggcggc
     1921 cacgacggcc aggagggggt tcagcgtcag ccccgctgcc gcgagggccc cgaggaagag
     1981 cgagaggttg tagggattgg tgaaggcgta cttgagatag gggcgcttct cgatgagggc
     2041 gtcgcgggcc gacgccgcga cggcctcgac gaccggcggc agctcccggt ccctggcggc
     2101 ggctgagcgg gagggcgggg ctgggtcggc ggcgcgcggg cctggcctct cgggtgcagc
     2161 catgcgcgta ttctacgcgg tcgcggcgcc cggcgcgtac agcatgtcga gcaggcgctt
     2221 ctcgaccgcc cggaattccg gcgccgactt gagcgccatg tcccggttct gaaagaaggg
     2281 cgtctccacc tgatgcagga tgcgcgccgg ccgtgccgac agcacgtaga tcgtgtcggc
     2341 gaggtacacg gcttccgaca cgtcgtgggt cacgaacagg atcgtgttgt ccatagtccg
     2401 cgacacgtcc agcagcaggc cctgcatgcc ccaccgcgtc tgagggtcga gggcgccgaa
     2461 gggttcgtcc atcaggatca cgcggggctg gttgatgagc gtgcgcgcga tcgccacgcg
     2521 ctgtttcatt ccgccggaca ggtccttcgg gaagcggttg gcgaattcgg tcagcccgac
     2581 cgaggcgagg tactggtcgg agagggcccg gcgctctgac gccggcatgc cctgcaggcg
     2641 gagcccgtac tcgacgttgt cgcggaccgt cagccatcca aacgacgtgt acgcctggaa
     2701 gaccatgccc cggtcgcgcc cggggccggt cacaggatgg ccgtccacga ggatgtcgcc
     2761 ggcgctcggg gggatcaacc cggcgaccgc cttcaggatg gtggacttgc cgcagccgga
     2821 cgggccgagg aacaccacga tctcgccggc gccgggcttg tcctcgatgt caaaggacac
     2881 gtcccggacg gcctcggtct cgtggccgtc gcggccgatg taagtgacgc cgaggccctt
     2941 cacgcggagc ttcggcggct tcgcggcgag gtccgcccgg taggtcggcg gcgcgccggc
     3001 ggttcggccg tcaaccgccg gggcggccga gccgggctcg cctggctccg cgccgcgcgt
     3061 ctgcccgctc atgccgtctg ctcccgccac cggaacagca tgctcgacgc cgtccggatc
     3121 agcccgtcgg tcagcagccc gatgccgccg atgaccagca ggcccgcgaa cgaccacgac
     3181 gccttctggt gcgtgcgcgc cagttcgacc atgtagccga ggccgtgctc cgggttgacc
     3241 gcctcggcca ggatcacgta ggtccacgag atggcgttca tcacgcggaa cgagtcgaag
     3301 atctcgggca gggccgccgg cacgaggatc gtgcggacga cctgccaccg gcccgctccg
     3361 agcgtcaacc ccgtctggac cagctcctcc gccaccgcgc ggatggccgt gacgacgacc
     3421 ggcagcaggt agacgaagac gcccaggaag aggaacgcga tcttctgctt ctcgtagatc
     3481 ccgaaccaca ggatgaggag cgggatgaac gccgagatgg gcatgtagcg cagcggcgcc
     3541 atgatcggct cgaaaagccg gttcacgggc tcgaaggcgc ccatgaggac ccccagcggc
     3601 agcgcgatgg ccgacgcgag caggaacgcg aagacgatgc gcttggtgga gatcaggatg
     3661 gcgctcgcca ggttgtgctg caggaacagc tgcagggtgc cgcgcacgac ctcgctcggc
     3721 gacggcagga agtccggcgg ggccagcccg ccgtacgagg cggccgacca ggcgcccagg
     3781 acgaccgccg gcatgacgag cccgaggatc aggctcgtcg agcgcggcag cggggtgcga
     3841 agggcgaaga gcggagacgt ccgtctggcc atcgcgctac tgggtggcca ggatgacctt
     3901 gatgtcggtg cgtcggttga gctgccgccc ggcctcggtc tcgttggagg cgacggggtt
     3961 ctcttccccg cgtccgatcg tctggaagcg cgccggctgg atgttcggga agtgcttcag
     4021 cacgtagttc ttcacggcca gcgccctccg ctccgagagc gtgaggttgg ccgcggccga
     4081 cccgaccgag tccgtgttgc cctcgatgcg caggtacgtg ttgccgaacg acgtcatcgt
     4141 ctcgcccagg gcgtccagga cgaagtacga gccctccatg atcacatccg aacccggcgt
     4201 gaagtggatc tggatctgct tgttgatgat ggcccggtcc gaggtcgacg ccttgcgggc
     4261 ggcaatcgcg ggctcctgga ccgtctggcc gggatacgcc tcggccagcg cggagatgaa
     4321 gcgcgtgtcg gcccagtccc tggcgtccac agggcgcgtg accaggccct tcttgcgcca
     4381 gatcacgaag gccgtgtcga acagcgtctc gtaatgcgcc ttgccgccgg tgagcccgta
     4441 gaactgcgcg ttgtcggcgt acggcgtgag cttcaggccg gagagcatgc cggacacggt
     4501 ctcgttgtcg agcttcagcg cccggccgac gaccgcgtag gaggaggcgg ggtccgcctt
     4561 catcagctcg atcccctcga accagccgtg cacgaagtcg cgcagcgtct cgggcgcgcg
     4621 gtcgatgaga tcctgccgcg cgcacagcgt gtcggcgatg atgttcgtcg cggccgtcgt
     4681 ggacacgagc acgtgggcct ctccctcccg cgccgcgacc gcgcctgaaa ggtccggctc
     4741 ccacgtgacg gcggcgtcca cctgcttggc cttgaacatc gcggctgccg ccggggcgtc
     4801 ctgcgtgaag atgatgcgct tctcaacggc cgcgcgatcc tcggccgaca ggcccgactg
     4861 cgccagcagg tagagcagca ggaagtgcga cggcgtgaac tgcgtgcagg cgatcttccg
     4921 gcccttcagc ccctcgatgg acgtgatcga cgcgagcgac acgattccgt cgccgccgcg
     4981 cgaccagtcc tgcatgatga tcgacttggc ctgctggttc tgttccgcca ggatcgacgc
     5041 ctcgcgcgcc caattgtcca cggtgttcca catgacgtcg atgtcgccgt tgcggaacgc
     5101 cgcgagcttc gcggccgggt cctccagcag cacgaacttc acgtcgaagc cgaagcgctt
     5161 gcggtagttg gaggcggggt tcgcgtccag tccggcgttg aagacgacgc cgggcgcgtg
     5221 gccggcccag gtgttgatgc cgacgacgag cggccggccc agcctgcccg acctggcaag
     5281 cgccgtgcgc gtcacgcccg tcgagcccgc cgtgcgggcc gggtccgcct gcatgccgcc
     5341 cagggcgctg aagtcgtcgc ggtcgacctg ctgcgcgggc tgcgggcccg cgcccttccc
     5401 gccctcggcc cactgcctga tctgcgcgcc cttgtagtgg tacgccgcgt agccgacgac
     5461 ggcgaggatg acgatcgtga tgaacagctt ggcgaacggt gtcagcttca tggtgcactc
     5521 cagcgggttc gcggcgcgtt ctcaggcgca cgccggccgc ggccccggtc cactacagcg
     5581 tcttctggcc ctgggcggcc tgctgggccg cacgcttcgc tttgagctcg gccagccggg
     5641 cccgggcctg cacctcgccc gcctggttcc gcagcgcgtc cagccgcgcg tcgagcgacg
     5701 actcggacag ctcgcgcccg aggttcgcct ccgcgatcgt cgtcctgatg tgctcgcgca
     5761 cgttgtcgag ggccttgacc tcggcgtcca ccgacagccc gtcgagctgt tcctggatct
     5821 tgaggcgcgc ctgggccgag tgcatcttgg ccagcatcga gtcgcgctcc gccttcagct
     5881 tcttgatctc ggcctggacc gtcagcaggg agttcttggc cgagtccgcg tccttctgcg
     5941 ccagctgcac gtcggcctgc agctccgcca gctccttctc cagctcgttc ttcttctgga
     6001 gcagcacgac ggccaggtcg tcctggttcg tgtcgagggc ggccgccagc tggccgtcca
     6061 cctcggccag ctcggccttc gccttctggt tgcgctggtc gatgtcctcg cgccgccgga
     6121 tgatggccgc cgtggccgtc ttcagcttcg tgtacttggc gatcatcgag ttgatcgcgt
     6181 tctcgtaggc tatctccggg tgctgctttt cgacgtccga gatccacagc gacaggaagc
     6241 cccgccacag gttgcccagg cgcgcgaaga accctccagc catgccgtca cctctccttg
     6301 ggagaaccgg gctcggccgc tccgccgccg gagccctgcg cggacgcggc gtgcaccacg
     6361 cgcgccacct cttcctgacc gaagaactcc aggatctgct ggatgcccag
//
LOCUS       DAOSIF010000128         6302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11203_length_6302_cov_3.277733, whole genome shotgun sequence.
ACCESSION   DAOSIF010000128 DAOSIF010000000
VERSION     DAOSIF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11203_length_6302_cov_3.277733"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PLT35_05785"
     CDS             <1..1165
                     /locus_tag="PLT35_05785"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOG28774.1"
                     /translation="IPMYFLIGVWGYERRVYAAVKFILFTMAGSVLMLVAILTVAYLH
                     SAATGAYSFGYEAWLRLALPKGTQVWLFLAFAVAFAIKVPLFPFHTWLPDAHVEAPTA
                     GSVILAGVLLKMGTYGLLRFAFPLFPQAAAEFAPALAVLAVVGIVYGALVAMVQPDLK
                     KLVAYSSVSHLGFVVLGIAAMNVQGVQGAVYQMLNHGVSTGALFLIVGMLSDRRHTRL
                     IAEFGGLKHVMPRLVAAFFVITLSSIGLPGLNGFVGEFLILLGAFRWNPWLAAAAASG
                     VILSAVYMLWMFQRVNYGSVTNPKNEGLPDMDRREGWVLVPAIAMAVFMGVAPWVFLR
                     PMTPAVERIVSRMAEAEPARAGTAPGREPGPADVPAALAVTGVEEEPGPSGPR"
     gene            1171..2625
                     /locus_tag="PLT35_05790"
     CDS             1171..2625
                     /locus_tag="PLT35_05790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOG28775.1"
                     /translation="MSTIGQVLNGIVPVMCVTLAGIACMLAEAFRRRGERMPIGGLGL
                     IGLAGAGASAVLLWGRRAGCLGVAEADDFGLFVQLVLVGIGVLTIVFSSDAVEREGLP
                     AGEYYALTMFALAGMMLMAVATDLLVIFLALEVFSLALYAMTGLRRRVYASAEGAFKY
                     FILGGFSSAFFLYGVAFAFGLSGSTHLDRLAAHVQAHAPAGGLLLLVAMGLLLAGFAF
                     KVAAVPFHMWTPDAYESAPTVVTGFMSTAVKAAAFAAFIRVFLHAFGPLRADWLPVVW
                     VLAICTMILGSVVGVVQVNLKRMLAYSSIAHGGYLLVALASGDEVGRAAILFYLVVYA
                     VTNVGAFAVIALLDSREAPMLTLDDYRGLWHRSPFLSGMLAVFLLSLGGFPPFAGFVA
                     KWYVFTAAVRADQYALAIVGVLTSVVSVFFYLRIIVMMYMADKAAEAAPAALPRVPKP
                     AAAALWLSVAALLYLGVLPTRVVEIAARAVASVF"
     gene            2833..4131
                     /locus_tag="PLT35_05795"
     CDS             2833..4131
                     /locus_tag="PLT35_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HOG28776.1"
                     /translation="MSDELFDDVVRRVPASAVDLDGEVRLETANRTQVELTPTDLEAL
                     LPPGHAARLVWRFVEGLDLSAFTATIRSREGRAGRPAIDPKILVALWLYATIDGVGSA
                     REVDRLCYTHDAYRWLRGGVSVNYHTLSDFRMGHHGALDQLLTQSITVLLDKQVVTLN
                     RITQDGTKIRASAGVRSFRRGERLRSCQQMARQQVARTRAEGDGVLPARRAAAQSRAA
                     EERLARVEEALAQLPEIEAAKRRNRVRSAPKASTTDPDARVMKMMDGGYRPAYNVQFA
                     ADVAGQAIVGVDVTNGSERGALLPMLAQVEIRTGRQPGSLLVAGDYVSAAGICGVAAR
                     GVTLYAPIPKGANAPGSGAGTTDPAVTAWRARMDTPEAKQIYRARAGTAERINGDTHR
                     HRAGGSIPVRGLTKVHTWALWAALAFNVMRVMEMVPHLMT"
     gene            complement(4419..5912)
                     /gene="selA"
                     /locus_tag="PLT35_05800"
     CDS             complement(4419..5912)
                     /gene="selA"
                     /locus_tag="PLT35_05800"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOG28777.1"
                     /translation="MSNFRVLPSIEQLRQRPRVAELVGRFGHTAVVEALREETDALRR
                     RLAEAASRPDSAASLPALPATAADANRLPATVEDAASAIEAGIEPRLARMFQPTLRPV
                     INATGVIVHTNLGRAPLCTAATDQVAAVGRRYSTLEYDLAAGGRGHRDHHAAGLLCRL
                     TGAESAVVVNNNAAATMLVLAAMAAGREVIVSRGELVEIGGGFRVPEVMEQSGAILRE
                     VGTTNRTRAADYDRAVSERTALILRVHRSNFTIEGFTEQPSLEELVAVGRRHGVLVAE
                     DLGSGNLIGGLAESAALAGLRADEMPWLEPFLRDEPTVRGSIGAGVDVVCVSGDKLLG
                     GPQAGIILGKRAPLDAIRRHPLMRALRVDKMTYAALTGTLMEYVTGRAADSVPVLRMM
                     TRSPESIEARARAVAGAVGAPGSFACEIVDGGSTIGGGTTPGLTLPTRLLALTHARLS
                     ADGLEAALRALDPPVIARIERDRVVLDLRTVFEDEDACLVRGLTAIV"
BASE COUNT          836 a         2164 c         2278 g         1024 t
ORIGIN      
        1 catcccgatg tacttcctca tcggggtctg gggctacgag cgccgggtct acgccgccgt
       61 gaagttcatc ctcttcacca tggccggcag cgtcctgatg ctcgtcgcca tcctcaccgt
      121 ggcctacctg cacagcgcgg cgacgggcgc gtacagcttc ggctacgagg cctggctgcg
      181 gctggcgctg ccgaagggca cgcaggtgtg gctcttcctc gccttcgccg tcgcgttcgc
      241 catcaaggtg ccgctcttcc cgttccacac gtggctgccc gacgcccacg tcgaggcgcc
      301 cacggccggc tccgtgatcc tggccggcgt gctgctgaag atgggcacct acggcctgct
      361 gcgcttcgcc ttcccgctct tcccgcaggc cgccgccgag ttcgccccgg cgctcgccgt
      421 cctggccgtc gtcggcatcg tctacggcgc gctcgtggcg atggtgcagc cggacctgaa
      481 gaagctcgtg gcctactcga gcgtgagcca cctggggttc gtcgtgctcg gcatcgcggc
      541 gatgaacgtg cagggcgtgc agggcgccgt ctaccagatg ctgaaccacg gcgtctcgac
      601 cggcgcgctc ttcctcatcg tcggcatgct gtccgaccgc cggcacacgc gcctcatcgc
      661 cgagttcggc ggcctcaagc acgtcatgcc gcgcctagtg gccgcgttct tcgtgatcac
      721 cctgtcgtcg atcgggctgc ccgggctcaa cgggttcgtc ggcgagttcc tgatcctgct
      781 cggcgcgttc cggtggaacc cgtggctggc ggccgcggcg gcgagcggcg tgatcctctc
      841 cgcggtctac atgctctgga tgttccagcg cgtgaactac gggtccgtga cgaacccgaa
      901 gaacgagggg ctgccggaca tggaccggcg cgaggggtgg gtgctcgtgc ccgccatcgc
      961 catggccgtg ttcatgggcg tggcgccgtg ggtgttcctg aggcccatga cgcccgccgt
     1021 cgagcgcatc gtgtcgcgga tggccgaagc cgagcccgcg cgcgccggga cggcgcccgg
     1081 gcgggagccg ggccccgctg acgttcctgc ggcccttgcc gtgacgggcg ttgaagagga
     1141 gccaggcccc tcgggcccca ggtgagcagc gtgtccacca ttggccaggt gctgaacggc
     1201 atcgttcctg tcatgtgcgt gacgctggcg ggcatcgcct gcatgctggc cgaggccttc
     1261 cggcgccgcg gcgagcgcat gcccatcggc ggcctgggcc tcatcgggct cgccggtgcc
     1321 ggcgcgtccg ctgtcctttt gtggggccgg cgcgccgggt gcctcggcgt ggcggaggcg
     1381 gacgacttcg ggctgttcgt gcagctcgtc ctcgtcggga tcggcgtcct cacgatcgtg
     1441 ttctcgtccg atgccgtcga gcgggagggg ctgccggccg gcgagtacta cgcgctcacg
     1501 atgttcgccc tcgccggcat gatgctcatg gccgtggcga cggacctcct cgtcatcttc
     1561 ctggcgctcg aggtgttctc gctggcgctg tacgcgatga ccggcctgcg gcgccgcgtc
     1621 tacgcgagcg ccgagggggc gttcaagtac ttcatcctgg gcggcttctc gagcgcgttc
     1681 ttcctgtacg gcgtggcgtt cgcgttcggc ctctccggca gcacgcacct cgatcggctg
     1741 gcggcccacg tgcaggcgca cgcgccggcg gggggcctgc tgctcctcgt cgccatgggc
     1801 ctgctgctcg ccggcttcgc cttcaaggtg gcggccgtgc cgttccacat gtggacgccg
     1861 gacgcctacg agagcgcgcc gaccgtcgtc accggcttca tgtccaccgc cgtgaaggcc
     1921 gccgccttcg ccgccttcat ccgcgtgttc ctccacgcgt tcgggccgct gcgggccgac
     1981 tggctgcccg tcgtgtgggt gctcgcgatc tgcaccatga tcctcggctc cgtcgtgggg
     2041 gtcgtgcagg tgaacctcaa gcggatgctc gcctactcga gcatcgccca cggcggctac
     2101 ctgctggtgg cgctcgcgtc gggcgacgag gtgggccgcg ccgccatcct gttctacctc
     2161 gtcgtgtacg ccgtgaccaa cgtcggggcc ttcgccgtga tcgcgctcct cgactcgcgc
     2221 gaggcgccca tgctgaccct cgacgactac cggggcctgt ggcaccgcag cccgttcctg
     2281 tccgggatgc tcgccgtgtt cctgctgtcg ctcggcggct tcccgccctt cgcggggttc
     2341 gtggcgaagt ggtacgtgtt cacggcggcc gtgcgcgccg atcagtacgc cctggccatc
     2401 gtcggcgtgc tgacgagcgt cgtctcggtg ttcttctacc tgcgcatcat cgtgatgatg
     2461 tacatggccg acaaggcggc cgaggccgcc ccggccgcgc tcccgcgcgt gcccaagccc
     2521 gccgcggcgg cgctgtggct gtcggtcgcg gcgctgctct acctcggcgt tctgccgacg
     2581 cgcgttgtcg agatcgccgc gcgcgccgtc gccagcgtgt tctaattcgg gacgcgggac
     2641 gcggggctcg ggattcggga cgcgggactc ggggctcggg actcgggact tggggctcga
     2701 gactcgggac tcggggttcg ggatcctgca gcgttctgat ggaggccggg tcttcagagc
     2761 ctgtgaagga atagtacatc gacgcggtga tttggcgttg cctgcggatc gtcgttgtac
     2821 taccctgcgt tcatgagcga cgaattgttc gacgacgtcg tgcgccgggt gccggcatcg
     2881 gcggtggact tggacggtga ggttcggctg gagacggcga atcggacaca ggtcgagttg
     2941 acgccgacgg atttggaggc gttgctgccc ccgggccatg cggcgcggct cgtgtggcgg
     3001 tttgtggagg ggctggacct gtcggcgttc acggcgacca ttcggtcgcg tgagggccgg
     3061 gcgggtcggc cggcgatcga ccccaagatc ctggtcgcgc tgtggctgta tgcgaccatc
     3121 gatggcgtgg gcagcgcccg cgaggtggat cgcctgtgtt acacgcatga cgcgtatcgc
     3181 tggctgcgcg ggggcgtgtc ggtcaattac cacacgctga gcgactttcg gatgggccac
     3241 cacggggcgt tggaccagtt gttgacgcag tcgatcacgg tgctgctgga caagcaggtc
     3301 gtgaccttga atcgcatcac gcaggatggc acgaagatcc gcgcgagtgc gggggtgcgc
     3361 tccttccgcc gcggggagcg gttgcgatcg tgtcagcaga tggcgcggca gcaagtggcg
     3421 cggacccggg cggagggcga tggggtgttg ccggcgcgcc gagccgcggc gcagtcccgt
     3481 gccgccgagg aacgcttggc gcgcgtcgag gaggcgctgg cgcaactgcc ggagatcgag
     3541 gcggccaaac ggcgcaatcg ggtgcgcagt gccccgaaag cgtcgacgac cgacccggac
     3601 gcgcgggtga tgaagatgat ggacggcggc tatcggccgg cgtataacgt ccaattcgcc
     3661 gcggatgtgg cggggcaagc catcgtgggt gtcgacgtga cgaacggcag cgaacgcggg
     3721 gcgttgctgc cgatgctcgc tcaagtcgag atccgcaccg gccgccaacc gggcagcctg
     3781 ctggtggctg gcgactatgt cagcgccgcc ggcatctgcg gtgtggcggc gcggggggtg
     3841 acgctgtacg caccgatccc caaaggggcg aacgcgccgg gcagcggagc gggcacgacg
     3901 gacccggccg tgacagcgtg gcgcgcccgc atggacacgc cggaggcgaa acagatctac
     3961 cgcgcccgcg cggggaccgc ggaacgcatc aatggcgaca cgcatcgcca tcgcgccggt
     4021 gggagcattc ccgttcgggg actcacgaag gtgcacacgt gggccctgtg ggcggcgctc
     4081 gccttcaatg tgatgcgggt gatggagatg gtgccccatc tgatgacgta acgtccgacg
     4141 cgcgaccggg tcgcgagccg cgccggcgta caagccccgc acggcccacg acggaacaac
     4201 gccgatggtt atgcacggcc atcggatcat aagagaccgg gtctatacgg caattcgttc
     4261 acacgctctg aagccccggc ctccacggat cacgcattcg gccaacagct gcggtgtgcg
     4321 ccggggctgg ggccgcgaca gccttggcga ctagcgccta gcgccaagtc ccaagcccct
     4381 ggctcgcctc gccgacccgc aggcctccac gagccaggct agacgatggc ggtgaggccg
     4441 cgaacgaggc acgcgtcttc gtcctcgaat acggtgcgca ggtcgagcac gacgcggtcg
     4501 cgctcgatgc gggcaatgac tggtggatcg agcgctcgaa gtgctgcctc gagaccatcg
     4561 gccgacaggc gggcgtgcgt cagcgccagc agcctcgtgg gcagcgtgag gccgggcgtg
     4621 gtgcctccgc caatggtcga gcccccgtcg acgatctcgc acgcgaacga gcccggcgcg
     4681 cccaccgccc ccgcgacggc tcgcgcgcgc gcctcgatgg actcgggcga ccgcgtcatc
     4741 atgcgcagca cgggcacgct gtcggccgcg cggccggtga cgtactccat cagggtgccg
     4801 gtcagcgccg cgtaggtcat cttgtccacc cggagcgccc gcatgagcgg atggcgccgg
     4861 atggcgtcga gcggcgcgcg cttcccgagg atgatgccgg cctggggccc gccgagcagc
     4921 ttgtcgccgc tcacgcacac gacgtccacg cccgcgccga tgctgccgcg aacggtcggc
     4981 tcgtcccgca ggaagggctc gagccacggc atctcatcgg cccgcagccc cgcgagcgcg
     5041 gccgactctg caaggccgcc gatgagattc ccgctcccga ggtcctcggc gaccagcacg
     5101 ccgtgccgcc ggccgacggc cacgagttcc tcgagcgacg gctgctcggt gaacccctcg
     5161 atggtgaaat tcgatcggtg cacccgcagg atgagcgccg tgcgctccga gacggcccgg
     5221 tcgtaatcgg ccgcccgcgt ccggttggtg gtgccgacct cccgcaggat cgccccggac
     5281 tgctccatga cctccggcac gcggaacccg ccgccgatct cgacgagttc cccgcgcgag
     5341 acgatgacct cccgccccgc cgccatcgcc gccagcacga gcatggtcgc ggcggcgttg
     5401 ttgttgacga cgacggccga ctcggcgccg gtcaggcggc agaggaggcc cgcggcgtga
     5461 tgatcgcgat ggccgcggcc gccggccgcg aggtcgtact cgagcgtgga gtagcggcgg
     5521 cccacggcgg cgacctggtc ggtggccgcc gtgcaaagcg gcgcgcggcc gaggttcgtg
     5581 tgaacgatca cgccggtggc gttgatgacg gggcgcagcg tcggctggaa catgcgcgcg
     5641 aggcgcggct cgatcccggc ttcgatggcc gacgcggcgt cctcgaccgt cgcgggcaac
     5701 cggttggcat cggcggccgt cgcgggaagc gcgggcagcg aggccgcgga atcgggccga
     5761 gaggcggcct ctgcaagccg gcggcgcagc gcgtcggtct cctcgcgcaa ggcctcgacg
     5821 acggcggtgt ggccgaagcg gccgacaagc tccgcgacgc gcggccgctg ccgcagctgc
     5881 tcgatggagg ggaggactcg gaagttggac atcggagcac tgactcggaa ttcagaatcc
     5941 ggaattcgga atccagagcc cggaacacgg gttgagccgc tgagccgcag cgggcacctt
     6001 ctcgcgtctg ggttccggct tctgaattcc ggattcctcc gtcgccgtct tgcgacccgc
     6061 ggcgcactca ggataccatc aggcgtccgc gcggtgatgg ccacggcgcg gccctcacgc
     6121 tcacatggcg gaatcgccca acgcgcacga tccgtccgct caggccagca gcgccgagaa
     6181 ccaggcagtg ccggcctacc gcccgcggga tcagttctgg ccgtacgccg atctcgacga
     6241 ggagccgtcc gacgaggagc tggcgcggct cgatccggac ctccaggccg cgctcctcga
     6301 gc
//
LOCUS       DAOSIF010000129         6274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11270_length_6274_cov_4.108217, whole genome shotgun sequence.
ACCESSION   DAOSIF010000129 DAOSIF010000000
VERSION     DAOSIF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11270_length_6274_cov_4.108217"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLT35_05805"
     CDS             <1..377
                     /locus_tag="PLT35_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28778.1"
                     /translation="DRRRAPASIQRHRQPGPLRPSAKGRTRVFRTAVNRTGRHAGLLA
                     FVEKAIKEKGGALRYSTPGVTLLRECQGRVTAVIARAPDGSYVRLDALLLQPRRCRFG
                     DEGDFMRTAGRALPRAAQRRLR"
     gene            462..959
                     /gene="nrfH"
                     /locus_tag="PLT35_05810"
     CDS             462..959
                     /gene="nrfH"
                     /locus_tag="PLT35_05810"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOG28779.1"
                     /translation="MSETRHLLWAGTLASVLVGAFAGTGIFTFFTAHGTAYLSDDPAA
                     CVHCHVMREEFDGWQHGSHHGVAVCNDCHLPHDNIAHKLFVKASNGYYHSKGFTLMNF
                     PEPIRIKPGNASVLEANCLRCHGEITEEIRAHGTLGVPTDPYQKADLYGCVRCHQETG
                     HGPTR"
     gene            1010..2506
                     /locus_tag="PLT35_05815"
     CDS             1010..2506
                     /locus_tag="PLT35_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOG28780.1"
                     /translation="MTDDRKPFYARPLVQMGMVAAVAAAATLALLALYSNISMRKSEA
                     KQRSMKLVELGETTVDPAEWGKNFPREYDSYLRTAEGYSTKYGGAGSEGLPLSRLQED
                     PRLPTIFDGYAFAIDFNRRRGHAYMLEDQRQTRRVTERPQPGACLHCHASNTVAYREA
                     GLKQGAPGTLDEPFTSPNGQAQLMAGFEAISKMSYADASKLVKHSVACIDCHDPRNMQ
                     VRVTKPGFIRGIDALARSSQPVPHLPSIEKWRKGDRSAAYDPNRDASRQEMRSMVCGQ
                     CHVEYYCGPKVTLFFPWHNGLKVEQIEATYDAYTFPDGHRFYDWSHGRTGAEVLKAQH
                     PEFEMWSQGIHARSAVACADCHMPYMREGAIKISDHQVRSPLLDVSRSCQTCHRFPEA
                     EMKARVEIIQDRTKALMDRAEDAVVALIGALQQARKDGANEAALQPVFALQRKAQWRV
                     DFVNAENSMGFHAPQEAARILAEAIDYARQGQLALRAQPPATGAKPGN"
     gene            2844..4238
                     /gene="rsxC"
                     /locus_tag="PLT35_05820"
     CDS             2844..4238
                     /gene="rsxC"
                     /locus_tag="PLT35_05820"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOG28781.1"
                     /translation="MVRLRLRDRSFRGGVHPRDEKALTRDLPLQDMPDPDVLLLPLQQ
                     HLGRPSVPAVKKGEWVREGQLLAEAQDGISAVVRAPRAGTITALQSGGTASGFPGRMI
                     ALKPGPPPSAADGEEARETAPVTLPALDPGAVAPADVVARTRAAGIVGQGGAAFPTAV
                     KLAPPQDAKLDVLIVNGCECEPYLTRDYRLMIERPAELVEGSALLARTLGVARVVIGI
                     ENNKLDAAEALRSAAAAPQRAAWTAGIAVEVLPLRTKYPQGAEKMLIEAATRRRVPPG
                     GLPMAVGAAIQNVGTVLAVRDAVVDGRVQTNAVLTVSGRAIRRPANLRVAVGTPIADV
                     IAHCGGLTDEVARIIVGGPMMGVAQHDLAAPVVKACSGILALTREELGPSLEPGNCVR
                     CGRCAEACPLNLVPSRLSRLAERQRFEEARDSGIEVCMECGTCAFTCPSHVPLVQWLR
                     LGKQEARKLGRRAS"
     gene            4235..5236
                     /locus_tag="PLT35_05825"
     CDS             4235..5236
                     /locus_tag="PLT35_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG28782.1"
                     /translation="MSAAPAALDQTGGRATPTLATSPHLHGWWTTPRMMWAVVAALVP
                     SLAAGLAFFGWQAAGVPLAAVAGSAAAEAAINRWRKLPFTLGDGSAVLTGLLLGLILP
                     PSLPLWMAGLGGIVSIGLGKAVFGGLGMNIFNPALVGRAFMQAAFPVSMTTWVANRLA
                     ADAVSAATPLALVKFREPGLGAVEVAPPIRDLFLGTIPGCLGETSALAILIGGGALLL
                     LKVANWRTPLAMAAGAALFGGALWACNPAQYPHPLFHLLSGGFLFGALFMATDLVTTP
                     ITGRGMAIFGFGAGFLTVLIRLFGGLPEGVMYSILIMNAAVPLINRWMRPRIFGAAR"
     gene            5233..5892
                     /locus_tag="PLT35_05830"
     CDS             5233..5892
                     /locus_tag="PLT35_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG28783.1"
                     /translation="MKTMLHMVATLVIVGLVAGASLSLVSGWAQPLIEANETKVKMAG
                     VMEVVPGGVSSKTLAEIASATPAPPGGDVEAYQVLDAQGQVLGWAVIGEGTGFSDKIR
                     LMVGVAPDFGRTLGIRILKDAETPGLGTKIREGDFPDRFFGRGGQAPSLGDGPLTVVK
                     APPGAANEVQAITGATISSKAVVKIVNEGVARLRARVGAGAAGGGTGAPAAPGRGEAG
                     R"
     gene            5889..>6274
                     /gene="rsxE"
                     /locus_tag="PLT35_05835"
     CDS             5889..>6274
                     /gene="rsxE"
                     /locus_tag="PLT35_05835"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOG28784.1"
                     /translation="MKARVSARQEVLKGLWDQNPVFRQLLGMCPTLAVTVTALNGVAM
                     GAAVVFVLVCSNLMISLVRKWIPGQVRIAAYIVIIATFVTVVDLTMKARFPELSSSLG
                     AFIPLIVVNCIILGRAEAFASKNGPWR"
BASE COUNT          916 a         2232 c         2244 g          882 t
ORIGIN      
        1 ccgatcgcag gcgagccccc gcctcgattc agcgccatcg ccagccaggg ccgctgcggc
       61 cgtctgcgaa gggccgtact agggtgttcc gcacggcagt gaaccggacc ggccggcacg
      121 ccgggctgct cgcgttcgtc gagaaggcca tcaaggagaa gggcggagcg ctccgctact
      181 cgacgcccgg cgtgacgctc ctgcgcgaat gccaggggcg cgtgacggcg gtcattgcca
      241 gggcgccgga cggcagctac gtgcggctcg acgcgcttct gcttcaaccg cgccgctgcc
      301 gcttcggcga cgagggcgac ttcatgcgga cggccggacg ggccctgccg cgcgccgcgc
      361 agcggcgcct ccgctagtgg agttccttgc cgacctgctc tagaatggcc gggccggcgc
      421 ggcgtttgtc gcacgcgtcc gcgcacgagg gacccgcggc cgtgagcgag acacgccatc
      481 tcctgtgggc gggcaccctg gcgagcgtcc tggtcggagc gttcgccggg acgggcatct
      541 tcacgttctt cacggcccac ggaacggcgt acttgtccga cgatccggcc gcgtgcgtgc
      601 attgccacgt catgcgcgag gagttcgacg gctggcagca cggctcccac cacggcgtgg
      661 ccgtctgcaa cgactgccac ctgccccacg acaacatcgc gcacaagctg ttcgtgaagg
      721 ccagcaacgg ctactaccac tcgaagggct tcaccctgat gaacttcccc gagcccatcc
      781 ggatcaagcc ggggaacgcg tcggtgctcg aagccaactg cctgcgctgc cacggcgaga
      841 tcaccgagga gatccgggcc cacgggacgc tgggcgtccc gactgatccg taccagaaag
      901 ccgacctcta cgggtgcgtg cgctgccacc aggagacggg gcatggcccg acgcgctgat
      961 catctgcacg gcgccgagcc tccgtgtccg acgccggagg gagtcaccca tgaccgatga
     1021 ccgaaagccc ttctacgccc gtcccctggt ccagatgggc atggtcgcgg cggtcgcggc
     1081 cgccgccacg ctggcgctgc tggcgctcta ctccaacatc tccatgcgga agtcggaggc
     1141 gaaacagcgc agcatgaagc tggtggaact cggcgagacg acggtcgatc ccgccgaatg
     1201 ggggaagaac ttcccccgcg agtacgactc ctacctgcgc acggccgagg gctacagcac
     1261 caagtacggc ggggccggca gcgagggcct gccgctcagc aggctccagg aagacccgag
     1321 gctcccgacg atcttcgacg gctacgcctt cgccatcgac ttcaaccgcc gccgggggca
     1381 cgcgtacatg ctcgaggacc agcgccagac caggcgcgtg accgagcgcc cgcagcccgg
     1441 ggcctgcctg cactgtcacg cctcgaacac cgtggcatac cgggaagcgg gcctgaagca
     1501 gggggccccc ggcaccctgg acgagccctt caccagcccc aacggccagg cgcagctgat
     1561 ggcgggcttc gaggcgatca gcaagatgag ctacgccgac gcctccaagc tcgtgaagca
     1621 ctcggtggcc tgcatcgact gccacgaccc caggaacatg caggttcgcg tcaccaaacc
     1681 ggggttcatc cgcggcatcg acgccctcgc ccggagcagc cagccggtgc cgcacctgcc
     1741 gtccatcgag aagtggcgga agggagaccg ttccgccgcc tacgatccca accgggacgc
     1801 ctcgcgccag gagatgcgat cgatggtgtg cggccagtgc cacgtggagt actactgcgg
     1861 cccgaaggtc accctgttct tcccctggca caacgggctc aaggtcgaac agatcgaagc
     1921 cacctacgac gcgtacacgt tcccggacgg ccaccggttc tacgactggt cgcacggccg
     1981 gaccggggcc gaggtgctga aggcccagca ccccgagttc gagatgtgga gccagggcat
     2041 ccacgcgcgc tcagccgtgg cctgcgccga ctgccacatg ccgtacatgc gcgagggcgc
     2101 catcaagatc agcgaccacc aggtccggag cccgctgctg gacgtctcgc ggtcctgcca
     2161 gacgtgccac cgattccccg aagccgagat gaaggcgcgg gtggagatca tccaggaccg
     2221 cacgaaggcg ttgatggacc gcgccgagga tgccgtggtg gcgctcatcg gcgcgctgca
     2281 gcaggcccgg aaggacggcg cgaatgaggc ggcgctccag ccggttttcg ccctgcagcg
     2341 caaggcgcag tggcgcgtcg acttcgtgaa cgccgagaac tcgatggggt tccacgcgcc
     2401 gcaggaagcg gcccgcatcc tggccgaggc catcgactac gcccgccagg gtcagcttgc
     2461 cctgcgcgct cagccgccgg cgaccggtgc gaagccgggc aactgagcgg cggcgccacg
     2521 cgtccggcgc ggccggaccg cggcgaagcc cgcgcgtgaa tcgatccgcc cgggcaaggc
     2581 ggctacggcc gcgcccccgg gccgggaagg cgcgggtgcg cgggaaacag cgcgcctcgg
     2641 gtatcctata aggtgagttg aagggccctg cgggtccgcc gaggggccgc gcgcggtctg
     2701 gaaggccgga tggcggccgc gcgtcggccc cgaatgcagg cgggcccgcg tcgagatatc
     2761 ggatccctac cgtcggcgat cgccgcgcca ccccccccgc gctgaggccg tacgtaggtg
     2821 cgtgccgtgt cgcggaggtt ggcatggttc gattgcgtct tcgagatcgg agtttccggg
     2881 gcggggttca tcctcgcgac gagaaggcgc tgacgcgcga cctcccgctt caggacatgc
     2941 ccgatccgga cgtgctcctg ctcccgctcc agcagcacct cggccgtccc tcggtgccgg
     3001 ccgtcaagaa aggcgaatgg gtccgcgagg gccagctgct ggccgaggcc caggacggca
     3061 tcagcgccgt cgtgcgggcg ccgcgggcgg gcacgatcac ggcgctccag tcgggcggca
     3121 cggccagcgg cttcccgggc cggatgatcg cgctcaaacc cggcccgccg ccctcggccg
     3181 ccgacggcga ggaggcgcgc gagaccgcgc cggtcacgct gccggccctc gacccgggcg
     3241 ccgtcgcgcc cgccgacgtg gtggcgcgca cgcgcgcggc gggcatcgtc gggcagggcg
     3301 gcgccgcctt cccgacggcc gtcaagctcg cgccgcctca ggacgcgaag ctcgacgtcc
     3361 tcatcgtgaa cggctgcgag tgcgagccgt acctcacgcg cgattaccgc ctgatgatcg
     3421 agcggccggc ggagctcgtc gagggctcgg ccctgctggc gcgaacactc ggcgtggcgc
     3481 gcgtcgtgat cggaatcgag aacaacaagc tcgacgcggc cgaggcgctt cgctcggccg
     3541 ccgcggcccc gcagcgcgcg gcctggacgg ctggcatcgc ggtcgaggtg ctgccgctgc
     3601 gcacgaagta cccgcagggc gccgagaaga tgctgatcga ggcggccacg cgccgccgcg
     3661 tgccgccggg cggcttgccc atggccgtcg gcgcggccat ccagaacgtg ggcacggtgc
     3721 tggcggtgcg ggatgcggtg gtggacggcc gcgtccagac caacgcggtg ctgaccgtca
     3781 gcggccgcgc gatccggcgt ccggccaacc tgcgcgtggc cgtcggcacg ccgatcgccg
     3841 atgtcatcgc gcactgcggc gggctcaccg acgaggtggc gcggatcatc gtcggcggcc
     3901 cgatgatggg cgtggcccag cacgacctgg cggcgcccgt cgtcaaggcg tgctcgggca
     3961 tcctggcgct gacgcgggag gagctcggtc cctcgctcga accgggcaat tgcgtcagat
     4021 gcggccgctg cgccgaagcc tgtcccctca acctggtgcc gagccgcctg tcgcgccttg
     4081 ccgagcggca gcgcttcgag gaagcccgcg actcggggat cgaggtctgc atggagtgcg
     4141 ggacgtgcgc cttcacctgc ccctctcacg tgccgctcgt gcagtggctg cggctgggca
     4201 agcaggaagc ccgcaaactg ggccggaggg cgtcatgagc gccgcgccgg ctgccctgga
     4261 tcagacgggc gggcgggcca cgccgacgct cgccacctcg ccgcacctcc acggctggtg
     4321 gacgacgccc cggatgatgt gggcggttgt cgccgcgctc gtgccgagcc tggcggccgg
     4381 ccttgcgttc ttcgggtggc aggcagccgg cgtgccgctc gcggccgtgg ccgggtccgc
     4441 agccgccgag gccgccatca accgctggcg caagctgccg ttcaccctcg gcgacgggtc
     4501 ggcggtgctg accggcctcc tgctcggcct gatccttccg cctagcctgc cgctgtggat
     4561 ggccggcctt ggcgggatcg tgtccatcgg cctgggcaag gcggtcttcg gcggcctggg
     4621 catgaacatc ttcaacccgg cgctggtcgg gcgggcgttc atgcaggcgg ccttcccggt
     4681 cagcatgacc acctgggtcg cgaacaggct ggccgccgac gccgtcagcg cggccacgcc
     4741 gctggcgctg gtgaagttcc gcgaaccggg cctcggcgcc gtggaagtgg cgccgccgat
     4801 tcgcgacctc ttcctcggga cgattcccgg ctgcctgggc gaaacaagcg ccctggccat
     4861 cctcatcggc ggcggcgccc tcctgctcct gaaagtggcc aactggagga cgcccctcgc
     4921 catggccgcc ggcgcggcgc tgttcggcgg cgcgctctgg gcctgcaacc cggcccagta
     4981 cccgcatccg ctgtttcacc tgctgtcggg cgggttcctg ttcggcgccc tcttcatggc
     5041 caccgacctc gtgacgacgc cgatcaccgg gcggggcatg gcgatcttcg ggttcggggc
     5101 gggcttcctg accgtcctga tccggctgtt tggagggctg cccgagggcg tcatgtactc
     5161 catcctcatc atgaacgccg ctgtccctct catcaaccga tggatgcgcc cccgcatctt
     5221 tggagccgcg cgatgaagac catgctgcac atggtggcca cgctggtcat cgtgggcctg
     5281 gtcgccgggg cctcgctgtc gctggtcagc ggatgggcgc agccgctcat cgaggccaac
     5341 gagacgaagg tcaagatggc cggcgtcatg gaagtcgtgc ccggcggggt ctcgtcgaag
     5401 acgctcgccg aaatcgcaag cgcgacgccg gctccgcccg gcggcgatgt cgaggcctac
     5461 caggtgctcg acgcccaggg gcaggttctc ggctgggcgg tcatcggcga gggcacgggg
     5521 ttctccgaca agatccggct gatggtcggc gtggcgccgg acttcgggcg cacgctcggc
     5581 atcaggatcc tgaaggacgc ggagacgccc ggcctcggca cgaagatccg cgagggcgac
     5641 ttcccggatc gcttcttcgg ccgcggcggc caggcgccca gcctcggtga cggcccgctg
     5701 accgtcgtca aggccccgcc gggagccgcc aacgaggtcc aggccatcac gggggcgacc
     5761 atcagttcga aggccgtcgt gaagatcgtc aacgagggcg tggcgcggct gcgggcgcgg
     5821 gtgggcgccg gggcggccgg aggcggaacg ggcgcgccgg cggcgccagg ccgcggggag
     5881 gcggggcgat gaaggcccgg gtgagcgcgc ggcaggaggt cctcaagggc ctgtgggatc
     5941 aaaacccggt gttccgccag ctgctgggca tgtgcccgac gctggccgtc acggtgacgg
     6001 ccctcaacgg cgtggccatg ggcgcggcgg tggtcttcgt gctggtctgc tcgaacctca
     6061 tgatcagcct ggtgcggaag tggatccccg gccaggtccg catcgcggcc tacatcgtca
     6121 tcatcgcgac cttcgtcacc gtcgtcgatc tcacgatgaa ggcgcgcttc cccgagctga
     6181 gcagctcgct cggcgcgttc atcccgctca tcgtcgtcaa ctgcatcatc ctcggccgcg
     6241 ccgaggcgtt cgcgtcgaag aacggcccgt ggcg
//
LOCUS       DAOSIF010000130         6177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11494_length_6177_cov_3.591473, whole genome shotgun sequence.
ACCESSION   DAOSIF010000130 DAOSIF010000000
VERSION     DAOSIF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11494_length_6177_cov_3.591473"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..680
                     /gene="rpiB"
                     /locus_tag="PLT35_05840"
     CDS             234..680
                     /gene="rpiB"
                     /locus_tag="PLT35_05840"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOG28785.1"
                     /translation="MRIAIGSDHAGFALKETIKALLDDLGVPFDDLGTASAEPADYPD
                     FAEAVARAVAAGRADRGILICGTGIGMSIAANKISGVRAALACTEASAKLGRAHNDAN
                     ILALGGRTTPAAAVPSIVRAFLATEFEGGRHGARVAKIAAIERKDP"
     gene            683..1954
                     /gene="glyA"
                     /locus_tag="PLT35_05845"
     CDS             683..1954
                     /gene="glyA"
                     /locus_tag="PLT35_05845"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG28786.1"
                     /translation="MEHILPARGLRLRDVDPDVADAIRNEAARQAGGLELIASENFVS
                     RAVLEATGSVFTNKYAEGYPGRRYYGGCDFADVVERLAVDRAKELFGADHANVQPHSG
                     AQANMAVYFTLVKPGETVLGMNLSHGGHLTHGHPLNFSGALYTIVPYGVRRDDERLDY
                     DEIERLAGQHKPKLIMVGASAYPRIIDFARIRGIADRVGAAMVTDMAHIAGLVAGGVH
                     PSPVPFADFVTSTTHKTLRGPRAGLVLCKAKWAKELDRSVFPGVQGGPLVHVIAAKAV
                     CFREALLPQFAAYQKKVVANARKLAEVIGSHGFRLVSGGTDTHLMLVDVFSKGLTGRE
                     ATAALGAAGITVNQNTIPFDENPPLKASGIRIGTPAVTTRGMGEAEMVLIGDLVARVL
                     DAPADGRAHAMVRAEVEALCRKFPLPADLPD"
     gene            2005..3924
                     /locus_tag="PLT35_05850"
     CDS             2005..3924
                     /locus_tag="PLT35_05850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOG28787.1"
                     /translation="MPAFEPRQGQREMAAAVARVFARGGVLLAEAGTGTGKTLAYLAP
                     AILSGRRVLVSTGTKNLQEQIYVKDIPLLREGLGIPFTAACMKGRGNYLCLHRFAAFR
                     DDIDAGSPADRVYLQMIDEWAASTETGDRAELADLPEDVPFWASISAAAENCVGPDCP
                     EHGACFVTRMRERAAAADVVIVNHHLLCADAAVRQGSYGEVIPDCPCAVIDEAHQLED
                     VATQYFGISVGAFRLAELVRDFDRVLASGEASDEGGELGRALNRVDSQARAFFAAAAA
                     LIWSAGPSAPAAAAGADERVRVTPRTLEPAQEPGLGLLDALDGLAGAVAAESQASEDL
                     AALGRRAADIADDLRFLLAANDRDYVYFLERRGRGVFLRAAPINVATLVRTQVLERFP
                     SAVLTSATLAVEGSFAYVRSRLGVERGEELLLPSEFDFASQALLYLPSRMPSPKTADF
                     PAAAGREVAEILRRTSGRAFVLFTSYAVLRSVHAALQEAIAFPVFVQGSLPRSALLAR
                     FRATPHAVLLATSSFWQGVDVVGEALSCVIVDRLPFASPGDPVVAARMEAIEAGGGDP
                     FSEYQVPLAVLALLQGLGRLIRHRRDRGVLAVLDPRLRTMPYGRRFLASLPPAPVTSE
                     LSDIERFFGVRTPLL"
     gene            4035..5009
                     /locus_tag="PLT35_05855"
     CDS             4035..5009
                     /locus_tag="PLT35_05855"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG28788.1"
                     /translation="MSRLMSGRPLLVLGLAALALFLLAQPSQAQTGAVSGRVVDGADK
                     PLEKAVVVIEFTDGINRRYEVKTNKKGEFIQIGLAPGNYKITAKYENLGEQSQTVRVR
                     LGDPTKVDIVLGGGPGGMTKEDAAKAAAVKALFDEGVALSKEHKYDEAIAKFTEASDV
                     TPGCFDCYYNIGFAHSQKKEYDQAEAAFLKAIELRPNYVDAYNGLATVYNAQKKFDKA
                     TEASQKAAQLAASAGPGTSGAGAVEAEYNQGVILWNAGKIAEAAEHFQQVILLKPDHA
                     DAHYQLGMAYVNQNKLAEAVPLFEKYLELAPDGQFAPTAKGVLASIKK"
     gene            5016..5726
                     /locus_tag="PLT35_05860"
     CDS             5016..5726
                     /locus_tag="PLT35_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG28789.1"
                     /translation="MDEPSRHGTIAASLSAVRQRLARSLERAGRSRADVRLVAVSKTR
                     PPEDIRAAVGAGQLDFGENRVQEALEKIGLTADMPISWHLVGHLQSNKARKAAAAFAW
                     IHSIDGVDLLRRVDEAAADAGRSPVVLVQVDLAGEPTKHGVAADACLPIFEAGAACRA
                     ASVGGLMVLPPFTEDPEEARPYFRRLRELRDALAGRGVPAAMLRELSMGMSHDFEVAV
                     EEGATVVRVGTAIFGERA"
     gene            5778..>6177
                     /locus_tag="PLT35_05865"
     CDS             5778..>6177
                     /locus_tag="PLT35_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOG28790.1"
                     /translation="MNVTPLDLRQQQFRTTMRGFDKDEVTAFLAEAADDYAEAIREAD
                     RLRQEVTRLEGLINEHREHERNLRNTLMTAQRLADEISDNAHKQAGTIIKDAESRAAL
                     ILEKAQSRYEDVQREIDGLRLKRREVENSVE"
BASE COUNT          921 a         2148 c         2226 g          882 t
ORIGIN      
        1 tccggacaac caccgtgtcg ccgccggcgg cccgggcctt cacgacgtcc gcgcggtaga
       61 cccaggggtg gccgccgcgg atccgatctt cgccgcgggc ggtgacaacg gcaacgggct
      121 cggtcggcgg cggggaggcg ctcatggctg ggtccgcgcc gacgggaaca cgggccgccg
      181 cgcgccctcc catcatgcgg aatccgccgc gggaaatcga gtagaatccg gctgtgcgca
      241 tcgcgatcgg ttcggaccac gccgggttcg cactcaagga aaccatcaag gcgctgctcg
      301 acgacctggg agtcccgttc gacgatctgg gaacggcgtc ggccgagccg gcggactatc
      361 cggacttcgc cgaggccgtc gcgcgcgccg tcgccgcggg gcgggcggac cgcggcattc
      421 tgatctgcgg gaccggcatc ggcatgagca tcgcggccaa caagatcagc ggcgtgcggg
      481 cggccctcgc ctgtaccgag gccagcgcga agctcgggcg cgcgcacaac gacgccaaca
      541 tcctggccct cggcgggcgc acgacgccgg ccgccgccgt cccgagcatc gtccgcgcgt
      601 tcctggccac cgagttcgaa ggcggacgcc acggcgcgcg cgtggcgaag atcgcggcca
      661 tcgaacggaa ggatccgtag ccatggaaca catcctgccc gcccgcggcc tgcgactgcg
      721 ggacgtcgat cccgatgtcg ccgacgccat ccgcaacgaa gcggcgcgcc aggccggggg
      781 cctcgagctc atcgcctcgg agaacttcgt cagccgcgcc gttctggagg ccaccgggtc
      841 ggtcttcacg aacaagtacg ccgaaggcta ccctggccgg cggtactacg gcggctgcga
      901 tttcgcggac gtcgtcgagc gcctggccgt ggatcgggcg aaggagctgt tcggcgcgga
      961 ccatgcgaac gtgcagcctc actccggggc ccaggccaac atggccgtct acttcacgct
     1021 ggtgaagccg ggcgagacgg tcctcggcat gaacctgtcg cacgggggcc acctgacgca
     1081 cgggcatccg ttgaacttct cgggcgcgct ctacacgatc gtgccctacg gcgtccgccg
     1141 ggacgacgag cggctcgact acgacgagat cgagcggctc gccggccagc acaagccgaa
     1201 gctcatcatg gtgggtgcca gcgcctaccc gcgcatcatc gactttgccc gcatccgcgg
     1261 catcgcggac cgcgtcggcg cggcgatggt caccgacatg gcgcacatcg ccgggctcgt
     1321 ggcgggcggc gtccatccca gcccggttcc cttcgccgac ttcgtgacga gcacgacgca
     1381 caagacgctg cggggcccga gggccgggct cgtgctgtgc aaggcgaagt gggcgaagga
     1441 gctggatcgc tcggtgttcc ccggcgtcca gggcggcccg ctcgtccacg tcatcgccgc
     1501 caaggcggtg tgcttccggg aggcgctcct gccgcagttc gccgcgtacc agaagaaggt
     1561 cgtcgccaac gcgaggaagc tggccgaggt catcggctcc cacggattcc ggctcgtgtc
     1621 cggcggcacg gacacgcacc tcatgctggt ggacgtgttc tccaagggcc tcaccgggag
     1681 ggaggcgacg gccgcgctgg gcgctgcggg catcacggtc aaccagaaca cgatcccgtt
     1741 cgacgagaac ccgccgctca aggcgagcgg catccgcatc ggcacgccgg ccgtgacgac
     1801 gcgcggcatg ggcgaggccg agatggtcct catcggcgac ctcgttgcgc gcgtcctgga
     1861 tgcgcccgcc gacggccggg cgcacgccat ggtgcgcgcc gaggtggaag ccctgtgccg
     1921 caagttcccg cttcccgcgg acctgccgga ctagacgagg ccgtcgagcg cgccttcgcc
     1981 gacgacggcc cgctggctcg ggccgtgccg gcgttcgagc cgcgccaggg gcagcgggag
     2041 atggccgccg ccgtcgcccg cgtcttcgcc aggggcggag tgctcctcgc cgaagccggg
     2101 acgggcacgg ggaagacgct ggcatacctc gcgccggcca tcctcagcgg ccggcgcgtc
     2161 ctggtgtcga caggcacgaa gaacctgcag gagcagatct acgtcaagga cattccgctc
     2221 ctgcgggagg gcctcggcat cccgttcacg gcggcctgca tgaagggccg cggcaactac
     2281 ctctgcctgc accggttcgc cgcgttccgc gacgatatcg acgccgggtc gcccgcggac
     2341 cgcgtctacc tgcagatgat cgacgagtgg gccgcgtcca ccgagacggg ggaccgggcc
     2401 gagctggccg acctgcccga ggacgtgccc ttctgggcgt ccatctcggc cgccgccgag
     2461 aactgcgtcg ggccggactg ccccgagcac ggcgcctgct tcgtcacgcg gatgcgcgag
     2521 cgcgccgcgg cggcggacgt cgtcatcgtc aaccatcacc tgttgtgcgc ggacgccgcg
     2581 gtccgccagg ggtcgtacgg ggaagtcatt ccggactgcc cctgcgcggt cattgacgag
     2641 gcgcaccagc tcgaggacgt ggccacgcag tacttcggga tctccgtcgg cgccttccgg
     2701 ctcgccgagc tggtccggga cttcgaccgg gtcctggcgt ccggggaggc gtcggacgaa
     2761 ggcggcgagc tgggccgcgc gctgaaccgg gtcgacagcc aggcgcgcgc cttcttcgcc
     2821 gcggccgcgg ccctgatctg gtctgccggg ccgtcggcgc ccgccgcagc ggcgggcgcc
     2881 gacgagcgcg tgcgcgtgac gccccggacg ctcgagccgg cgcaggaacc cgggctcggt
     2941 ctgctcgatg cgctcgacgg cctcgccggg gcggtcgcgg ccgagagcca ggcatccgag
     3001 gatctcgccg cgctcggccg ccgcgccgcc gacatcgcgg acgatctgcg cttcctgctc
     3061 gccgccaacg accgcgacta cgtctacttc ctcgagcgcc ggggccgcgg cgtgttcctt
     3121 cgcgcggcgc ccatcaacgt ggcgacgctc gtccgcacgc aggtgctcga gcgctttccg
     3181 tcggccgttc tcacgtcggc gaccctcgcc gtcgaaggct cgttcgccta tgtgcgcagc
     3241 cggctgggcg tcgagcgcgg ggaggagctc ctgctcccgt ccgagttcga tttcgcctcg
     3301 caggccctgc tctacctgcc gagccggatg ccgtcgccga agaccgcgga cttccccgcc
     3361 gccgctgggc gcgaggtggc cgagatcctg cgccggacga gcggccgggc cttcgtgctg
     3421 ttcacgagct acgccgtgct gcgctccgtc cacgccgcgc tgcaggaagc catcgcgttc
     3481 ccggtgttcg tgcagggctc gctgccgagg tccgcgctgc tcgcccggtt ccgggcgacg
     3541 ccgcacgccg tgctgctggc cacgtcgagc ttctggcagg gcgtggacgt cgtcggggag
     3601 gcgctgagtt gcgtcatcgt cgaccggctg ccgttcgctt cgcccgggga tccggtggtg
     3661 gccgcccgga tggaggccat cgaggccggc ggcggcgatc cattttccga gtatcaggtt
     3721 ccgctggccg tcctggccct cctgcagggc ctcggccgct tgatccggca ccgccgggat
     3781 cggggggtcc tggccgtgct cgatccgcgc ctgcgcacga tgccgtacgg gcggcggttc
     3841 ctggcgtcgc tccccccggc gcccgtgacc tcggagctct ccgacatcga gcggttcttc
     3901 ggcgtccgga cgccccttct ctgatcgcgg gactcggcat ttgggtgtaa actacgcaaa
     3961 gatcggcgaa ccgccggttc tgcgtgatgc cttgaccgga atcatccgcg tcgttacttg
     4021 ctagaggagt gccaatgtcc cgcctgatgt ccggccgtcc gctgctcgtg ctcggcctcg
     4081 ccgcgctcgc gttgttcctg ctcgctcagc ccagccaggc ccagaccggg gccgtgtcgg
     4141 gcagggtggt ggacggcgcg gacaagcccc tggagaaggc cgtcgtcgtc atcgagttca
     4201 ccgacggcat caaccgccgc tacgaggtca agaccaacaa gaagggcgag ttcatccaga
     4261 tcggcctggc gcccggcaac tacaagatca cggcgaagta cgagaacctg ggcgagcagt
     4321 cgcagaccgt gcgcgtgcgc cttggcgatc cgacgaaggt ggacatcgtg ctcgggggag
     4381 gccccggggg catgaccaag gaggacgccg cgaaggcggc ggccgtgaag gcgctgttcg
     4441 atgagggtgt cgctctgagc aaggagcaca agtacgacga ggccatcgcg aagttcaccg
     4501 aggcgtcgga cgtcacgcct ggctgcttcg actgctacta caacatcggg ttcgcgcact
     4561 cccagaagaa ggaatacgac caggccgagg cggcgtttct gaaggccatc gagctgaggc
     4621 ccaactacgt cgacgcgtac aacggcctgg cgacggtgta caacgcgcag aagaagttcg
     4681 acaaggcgac ggaggcgagc cagaaggccg cccagctggc tgcgtcggcc gggcctggca
     4741 cctcgggcgc gggcgccgtt gaggccgagt acaaccaggg cgtcatcctc tggaacgccg
     4801 gcaagatcgc cgaggccgcg gagcactttc agcaggtcat cttgctgaag cccgatcacg
     4861 ccgacgccca ttaccagctg ggcatggcct acgtgaacca gaacaagctg gcggaggcgg
     4921 ttccgctgtt cgagaagtac ctcgaacttg cgcccgacgg gcagttcgcg ccgacggcca
     4981 agggtgtgct ggccagcatc aagaagtagc ccgccatgga cgagccgtcg cgacacggca
     5041 cgatcgcggc gagcctctcg gcggtccggc agcggctggc tcgctccctg gagcgggccg
     5101 gccgttcgcg tgccgatgtg cggctcgtcg ccgtctcgaa gacgcgcccg ccggaggaca
     5161 tccgggcggc ggttggcgcg ggccagctgg atttcgggga gaaccgggtc caggaggcgc
     5221 tggagaagat cggcctgacg gccgatatgc cgatcagctg gcatctcgtc gggcatctcc
     5281 agtcgaacaa ggcgcggaag gccgcggcgg cgttcgcgtg gattcactcg atcgacggcg
     5341 tggatctgct gcggcgggtg gacgaggcgg cggccgacgc ggggcgcagc ccggtggtgc
     5401 tggtgcaggt ggatctggcc ggcgaaccga ccaagcacgg cgtggccgcg gatgcgtgcc
     5461 tcccgatttt cgaggccggg gcggcgtgcc gcgccgccag cgtgggaggc ctgatggtgc
     5521 tgccgccgtt cacggaagac ccggaggagg ccaggccgta cttccgccgg ctccgggagt
     5581 tgcgcgacgc gctcgccggc cgcggcgtcc cggcggcgat gcttcgcgag ctgtcgatgg
     5641 gcatgagcca cgacttcgag gtggccgtcg aggaaggcgc gaccgtcgtg cgtgtcggga
     5701 cggccatctt cggcgagagg gcgtagatga ccggccgccc tgacggcgcc ggccgcacca
     5761 ggttgcagga gaggacgatg aacgtcaccc cgctcgacct ccgccagcag cagttccgca
     5821 cgacgatgcg cgggttcgac aaagacgagg tcacggcgtt tctcgccgag gcggccgacg
     5881 actacgccga agccatccgc gaggccgatc ggctgcggca ggaggtgacg cgcctcgagg
     5941 gcctgatcaa cgagcaccgc gagcacgagc ggaacctgcg caacacgctg atgacggccc
     6001 agcgcctcgc cgacgagatc agcgacaacg ctcacaagca ggccggcacg atcatcaagg
     6061 acgccgagtc ccgtgccgcc ctgatccttg agaaggccca gtcgcggtac gaggacgtgc
     6121 agcgcgagat cgacggtctc cgcctgaagc gccgcgaggt ggagaactcg gtggagg
//
LOCUS       DAOSIF010000131         6163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11533_length_6163_cov_3.510478, whole genome shotgun sequence.
ACCESSION   DAOSIF010000131 DAOSIF010000000
VERSION     DAOSIF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11533_length_6163_cov_3.510478"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PLT35_05870"
     CDS             complement(<1..1044)
                     /locus_tag="PLT35_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG28791.1"
                     /translation="MIRAIIRFSAHNRLLVLMAASVAVAYGIYTLRHIPVDAIPDLSD
                     TQVIVYSRWDRSPDIIEDQVTYPIVSALLGTPKVKSIRGFSDFGFSYAYVIFEDGTDI
                     YWARSRVLEYLSKIQPVLPEGVKTELGPDATGVGWVFQYALVDDSGTQSLADLRALQD
                     WNLRYRLQSVPGVAEVASIGGFVQQYQVTVDPARLKAYDLGIMEVAEAVRRSNSEVGG
                     RLLEFAGREFMVRGRGYVKAAADIEKVVLKTDARGTPVLLRDVASVAVGPEIRRGVAD
                     LDGQGDAVGGIVVMRHGENARDVIDRVKARLGELEPSLPKGVRFVTTYDRSDLITRAI
                     DVLRHELLVEIAIV"
     gene            complement(1054..2754)
                     /locus_tag="PLT35_05875"
     CDS             complement(1054..2754)
                     /locus_tag="PLT35_05875"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG28792.1"
                     /translation="MTRTTRISLAVAAVLTLAAVSTWRVAVRSPERHGPSAGAPAAKY
                     QCPMHPTVISDRPGDCPICGMKLVPIKTAEQPPTGAAGSAHAASERYMCPMHPTVISD
                     RPGDCPICGMKLGLVPAALLKGELKAPGGAVPTYFCPMHPTVVSDKPGECPLCQMDLE
                     PIPQSLKTGWPEAGGGRRILYYRSPMDPAVRSDKPAKDSMGMDFVPVYEDQADPGAGS
                     VPGRSAVALSPERRSLLDVRSDEVREIRIERAIRTVGRVGVDERRLAHVHAKSEGYVE
                     RLYADFTGKYVRKGEPLLSIYSPELIATQQEYLLALRAEKELGASGIPSVATGGANLA
                     EAARERLRLWDISPADIAEIERTGTVRRAFDLRADAGGFVMAKNVVQGMRVMPADTLF
                     EIADLSSVWVLADVYESDLSAIRLEMPATITFAYEPGRAWRGRVSNIAPAVDGATRTV
                     KVRIDVPNRSGILKPDMFGDVQLLAAEGRGLVVADSAVIDAGDRKLVFLDRPDGTIEP
                     RAVEVGAKLADGYQVLRGLARGDRVVTSANFLLDSESSLKAALAAIAARTQPSADGTR
                     "
     gene            complement(2765..4144)
                     /locus_tag="PLT35_05880"
     CDS             complement(2765..4144)
                     /locus_tag="PLT35_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG28793.1"
                     /translation="MWTFSRRRDVAAWSLVAALVAGASGQVRAQGPAPPAQPDPVLEA
                     LVAEALANNPDVRTTESAVAAAQAAIPGARALPNPMLSMTFTNDGWAPSLGTMPMTTM
                     GIMVSQGLPWPGKRGLRADLATVQVRMLEPAVERARLGIEAGVKRAYYGLLLARELEA
                     IVTEQRRLWQQIEIATRARYAAGQGVQPDVLRIQAEVTRIDQQAIEQAAEADVRLAEI
                     NRLLARPLDTPVETPAKLRLAPITESFEEVADAARGRSPELQAARLAIEASEAAAALA
                     RRELRPDLTVQGGYMNRGGLDPMWQAGIGVSWPIDTRRRRSEIAEAEIAAKGGRHRSG
                     SLELQLAYRTRERYTRAKAAERLVALYDGGIVPQDQMTVESALANYQTGNVPFASVLE
                     AITALYADRWARAGLVAEHARLVASLYEASVDDASAMGPSGAPAAARIRTAASGSVAG
                     GMSGGMSGR"
     gene            complement(4722..5513)
                     /locus_tag="PLT35_05885"
     CDS             complement(4722..5513)
                     /locus_tag="PLT35_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28794.1"
                     /translation="MTSGTRAILVDHTTTALAQIPAQWVERARAMLHIAYGHTSHGSQ
                     LTTGMTGLAAFKGPAYAWRAGGAGGALDLRDTPFSGASDLGNPDRAAWAAATRTYLAA
                     QPAVNVVIWSWCGQVSSATPADIDAYLSAMSALEQEFPKVRFVYMTGHLDGTGLAGNL
                     HLRNEQIRQHCQSRGKVLYDFADIETYDPDGVYYGDRIPNDACDYDSNGDGRRDRNWA
                     VDWQGRHTVGVDWFQCPAAHSQPVNANMKAYAAWWLFARLAGWEG"
     gene            5829..>6163
                     /locus_tag="PLT35_05890"
     CDS             5829..>6163
                     /locus_tag="PLT35_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG28795.1"
                     /translation="MICRRLVLSMVFVLLLVPQAFAQALPARQGLPPGLRPAPPPQAA
                     LAAVPPEAVGLSSERLERLKAVMQQHVDQGRIAGVVTLIARGGRLAHFETYGKMDAEQ
                     NRPMRKDVIL"
BASE COUNT          880 a         2182 c         2157 g          944 t
ORIGIN      
        1 gacgatggcg atctcgacca ggagctcgtg gcggagcacg tcgatggccc gcgtgatgag
       61 atccgaccgg tcgtacgtgg tgacgaagcg cacgcccttc ggcagcgagg gctcgagctc
      121 ccccagccgg gccttgaccc gatcgatgac gtcgcgggcg ttctcgccgt ggcgcatgac
      181 gacgatgccg cccactgcgt cgccctggcc gtcgagatcg gccaccccgc ggcggatctc
      241 gggcccgacc gccaccgacg cgacgtcgcg caggagcacc ggcgtgccgc gggcgtcggt
      301 cttcagcacg accttctcga tgtccgccgc cgccttcacg tagccccggc cccgcaccat
      361 gaactcccgg cccgcgaact cgaggaggcg tcccccgacc tcgctgttgc tccggcgcac
      421 ggcctcggcc acctccatga tgccgaggtc gtaggccttg agccgggcag gatccacggt
      481 gacctggtac tgctgcacga agccgccgat cgaggcgacc tcggccacgc ccggcacgga
      541 ctgcagccgg tagcgcaggt tccagtcctg gagcgcccgg aggtccgcca gcgactgcgt
      601 ccccgaatcg tccacgagcg cgtactggaa gacccatccc acgcccgtcg cgtcgggccc
      661 gagctcggtc ttcacgccct cgggcagcac gggctggatc ttcgacagat actcgagcac
      721 gcggctgcgc gcccagtaga tatcggtgcc gtcctcgaag atcacgtagg cgtagctgaa
      781 cccgaagtcc gagaagcctc gaatgctctt caccttcggc gtgccgagca gcgccgacac
      841 gatcggatag gtgacctggt cctcgatgat gtccgggctc cggtcccagc gcgaatagac
      901 gatgacctgc gtgtcggaca ggtccggaat cgcgtcgacc gggatgtgac ggagggtgta
      961 gatgccgtag gccaccgcga cagacgcagc catcagcacc agcaggcggt tgtgggcgct
     1021 gaaacggatg atggctcgga tcatggccgg tcctcagcgc gttccgtcgg ctgacggctg
     1081 agtccgggcg gctatcgccg cgagggccgc cttgaggctc gactcggagt cgagcaggaa
     1141 gttcgccgac gtcaccacgc ggtcgcccct cgccaggccg cgcaggacct ggtagccgtc
     1201 cgccagcttc gcgcccacct cgaccgcgcg gggctcgatg gtgccgtccg gacgatcgag
     1261 gaagacgagc ttccggtcgc cggcgtcgat gacggcgctg tcggccacga cgaggccgcg
     1321 tccctccgcc gccagcagct gcacgtcgcc gaacatgtcc ggcttgagga tcccgctccg
     1381 gttgggaacg tcgatgcgca ctttgaccgt ccgcgtggcc ccgtccacgg ccggcgcgat
     1441 gttggatacg cgaccgcgcc atgcgcggcc gggctcgtag gcgaaagtga ttgtggccgg
     1501 catctccagc cggatggcgg agagatcgga ctcgtacaca tcggcgagca cccacacgga
     1561 cgacaggtcc gcgatctcga agagcgtgtc tgccggcatg acccgcatgc cctgcacgac
     1621 gttcttcgcc atcacgaagc cgccggcatc cgcacgaagg tcgaatgccc gccggaccgt
     1681 tccggtgcgt tcgatctcgg cgatgtcggc cgggctgatg tcccacagcc ggagccgttc
     1741 cctggcggcc tctgccaggt tcgcgccgcc tgtggcgacc gacggaatcc cgctggctcc
     1801 gagttccttc tcggcgcgca gggccaggag gtactcctgc tgcgtggcga tgagttcggg
     1861 gctgtagatc gaaagaagcg gctcgccctt ccggacgtac ttgccggtga agtccgcgta
     1921 gaggcgctcg acatagccct cggacttggc gtgcacgtgc gccaggcggc gctcgtcgac
     1981 ccccacccgg ccgaccgtcc gaatcgcccg ttcgatgcga atctcccgaa cctcgtcgct
     2041 ccgaacatcg aggaggctgc gccgctccgg cgagagcgcg acggctgagc ggccgggcac
     2101 ggagcctgcg cccgggtcgg cctggtcttc gtacacgggc acgaagtcca tgcccatgct
     2161 gtccttggca ggcttgtccg agcgcactgc cggatccatc ggcgaacggt agtagaggat
     2221 gcgccgcccg ccgccggcct ccggccatcc cgtcttcagc gactgcggga tcggctcgag
     2281 atccatctgg cacagcgggc actcgcccgg cttgtcggag accaccgtcg ggtgcatggg
     2341 gcagaagtac gtggggacgg ccccgcccgg cgccttgagc tcgcccttca gcagcgccgc
     2401 gggcacgagc ccgagcttca tgccgcagat cgggcagtcg cccggccggt cggagatgac
     2461 cgtcgggtgc atcgggcaca tgtacctctc cgacgcggcg tgcgccgatc ctgccgctcc
     2521 ggtcggcggc tgttcagcgg tcttgatcgg caccagcttc atgccgcaga tcgggcagtc
     2581 gcccggccgg tcggagatga ccgtcgggtg catagggcac tggtacttgg ccgctggagc
     2641 tccggcggac gggccgtgcc gctcgggtga gcggaccgcg acccgccagg tcgagaccgc
     2701 agccagagtc aggacggccg ctactgcaag gctgatccgg gtcgtgcgtg tcatgttctg
     2761 cgccctacct gccgctcatg ccgccgctca tgccgccggc cacgctgccg gacgcggccg
     2821 ttcgtatgcg cgccgccgcc ggcgcacccg atggccccat cgccgaggcg tcatcgacgc
     2881 tggcctcata gaggctcgcc acgaggcgcg cgtgctcggc gaccagcccg gcacgcgccc
     2941 accggtcggc gtagagcgcc gtgatcgcct cgagcaccga cgcgaacggc acgttgccgg
     3001 tctggtagtt cgcgagggcg gactcgaccg tcatctggtc ctgcgggacg atcccgccgt
     3061 cgtacagcgc gacgagcctc tcggcggcct tcgcgcgggt gtagcgctcg cgcgtcctgt
     3121 aggccagctg cagctcgagc gatcccgagc gatggcggcc gcccttggcg gcgatctcgg
     3181 cctccgcgat ctcgctccgg cgccggcgcg tatcgatggg ccacgacacg ccgatgccgg
     3241 cctgccacat cgggtcgagc cctccgcggt tcatgtaccc gccctggacg gtgaggtccg
     3301 gcctcagttc gcgtcgggca agggccgcgg cggcctcgct cgcttcgatg gccagccgcg
     3361 ctgcctgcag ttcaggactc cggccgcgcg cggcgtcggc gacctcttcg aacgactcgg
     3421 tgatgggcgc gagccggagc ttcgccggcg tctcgactgg cgtgtcgaga ggacgagcga
     3481 gaaggcgatt gatctcggcg aggcgcacgt cggcctcggc ggcctgttcg atggcctgct
     3541 gatcgatgcg cgtcacctcc gcctggatcc ggagcacatc cggctggact ccctgcccgg
     3601 cggcgtaccg cgccctggtc gcgatctcga tctgctgcca tagccgccgc tgctccgtga
     3661 cgatggcctc gagctcgcgc gccaggagca gaccgtagta cgcccgtttc acccccgcct
     3721 cgatgcccag acgcgcgcgc tcaactgcgg gttcgagcat ccggacctga accgtcgcca
     3781 ggtcggctct gagcccgcgc ttgcccggcc acggcagtcc ctggctcacc atgatcccca
     3841 tcgtcgtcat cggcatcgtg ccgaggcttg gcgcccagcc gtcgttggtg aacgtcatcg
     3901 acagcatcgg gttggggagc gcgcgtgcgc ccggaattgc agcctgcgcg gcggcgacgg
     3961 cggactcggt cgtccgcacg tccggattgt tcgccagggc ttcggcgaca agcgcctcga
     4021 ggacggggtc gggctgggct ggcggcgccg gaccctgcgc ccgcacctgt ccggacgcgc
     4081 cggccacgag tgcggcgaca aggctccacg ccgcgacgtc acgcctacgt gagaacgtcc
     4141 acatgtccgc ctccaacggc gggctggcga ggggacaggt cgccggaccg cccggttcag
     4201 cgatgtgcgg gaacacgcct ccggaggtcc gaacggacct ccggcatcga cgccggatta
     4261 gagcctgagg atgcgagtgg gaggagaggg agcggcgggc ggctcgtcca ggagatgcag
     4321 gcggacacct ccggcgtcgg cggcccattc ggcttctgtt gccggtgacg cctggatctg
     4381 gatggcgtcg acgggcgcca agtcccactg cgcggcctcg atgtggcagc aggactcgtc
     4441 cgctccaatc gcgcccgagg cgtcatcgcc gggcgagggg aggcagcagc tgtgaccggg
     4501 ccgcgagacg ggcgcgtccg ggccgtgagg gcagaaacac aggcccgccg tcgcattcac
     4561 cgacgctgcg accagcgtta caactccgag aatcctgagg aggcggccca cgatttccac
     4621 tatgcggagc cgagagccgt tctgtcaagc cggacgtcgt cgcgtgccgc gcgccgccga
     4681 gccggatccc gccgcgcggg ctgtcgtcag gcggtgacgc ctcatccttc ccatccggcg
     4741 agccgtgcga acagccacca ggcggcgtag gccttcatgt tcgcgttgac cggctgcgag
     4801 tgggcggcgg ggcactggaa ccagtccacg cccacggtgt ggcggccctg ccaatcgacg
     4861 gcccagttgc ggtcgcgcct gccgtccccg ttcgagtcgt agtcgcaggc gtcgttggga
     4921 atccggtcgc cgtagtagac gccgtccgga tcgtacgttt cgatgtcggc gaaatcgtac
     4981 agcaccttcc cccgagactg gcagtgctgc cggatctgct cgttgcgcaa gtgcaggttg
     5041 cctgcgaggc cggtgccgtc gagatggccg gtcatgtaca cgaaccgcac cttcgggaac
     5101 tcctgctcca gcgcgctcat ggccgagaga taggcgtcga tgtcggcggg ggtcgcgctg
     5161 cttacctggc cgcaccacga ccagatcacc acgttgactg cgggctgcgc ggccaggtaa
     5221 gtcctcgtcg ccgccgccca ggccgcgcgg tcggggttgc cgagatcgga cgcgcccgag
     5281 aacggcgtgt cgcgcaggtc gagcgcgccg ccagcgccgc cggcgcgcca ggcgtaggcc
     5341 gggcccttga aggccgccag gccggtcatg ccggtggtca actggctgcc gtgcgaggtg
     5401 tggccgtagg cgatatgcag catcgccctg gctcgctcca cccactgggc agggatctgc
     5461 gctaacgcgg ttgtcgtgtg atcgacgaga atcgcgcggg ttccggacgt cattggcggg
     5521 ggcgccggtg ccgccggacc ggttgccgtg ggtccggctg gagtcgactc atccgtgcgg
     5581 ccgcatgcgg cggcagcggc cagccctgcc agcgtggcga tcgccagcca cgtgcgcgtg
     5641 agactccgat tcatggtggg gcccattcta gttcgaggaa ccagctgcgc ccgttccggt
     5701 ggagccgcgg gcgccggccc cacgatcgtg caagctccgc gccaccaggc gccgccggag
     5761 ccgaccgtca aggcgcggcc cgcgtataat ccgccaagca tcgcctggcg agagggaggg
     5821 gcatcccgat gatctgcaga cgactcgtgc tctcgatggt gttcgtgctc ctgctggttc
     5881 cgcaggcgtt cgcccaggcg cttccggcgc gccaagggct gccgcctggc cttcgccccg
     5941 cgccgccgcc gcaggccgcc ctggcggcgg tcccgcccga ggccgtcggg ctgtcgagcg
     6001 agcgcctcga gcgcctgaag gccgtgatgc agcagcacgt ggaccagggc cgcattgccg
     6061 gcgtcgtgac gctcatcgcc cgaggcggca ggctggcgca cttcgagacc tacggcaaga
     6121 tggacgccga acagaaccgg ccgatgcgga aggacgtcat cct
//
LOCUS       DAOSIF010000132         6157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11543_length_6157_cov_3.875778, whole genome shotgun sequence.
ACCESSION   DAOSIF010000132 DAOSIF010000000
VERSION     DAOSIF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11543_length_6157_cov_3.875778"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2376
                     /locus_tag="PLT35_05895"
     CDS             <1..2376
                     /locus_tag="PLT35_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOG28796.1"
                     /translation="HLGQCGQPDVIGERHQPSQALPLGDGRTGVMVFGGPSSSRFLLN
                     DSTLWSGGPVDPAMNPDAIAWLPKVREALFAGDYRLATELTRKLQGRFSESYAPLGDL
                     YLDMPSVAAGSVSAYRRELDIAAGVARTSFKAGLTAIAQEAFVSHPARTLLVRLTASA
                     PGALAFSLRFESQLKHAAAVDASGDLLMTGRAPVRAEPNYRGNIKDPIVYDEGPDPKG
                     MRFAARARVLRTDGRIERGPSSLAVAGAREALVAIALDTSFAGFDREPSGGPDPVPAT
                     RATLDAAARQPYAALRLAHARDVEALMSRVALDLGRPARDEIPTDVRLREYADGAGDP
                     GLEALYFQFGRYLLVAGSRPGSPALNLQGIWNPHMRPPWSSNYTININTEMNYWPAET
                     TNLAECHGPLLRFIGDVARTGAVTARHYYGAGGWAAHHNTDLWAMSNPVGDFGQGDPV
                     WANWPMGGVWLARHLWEHYAFSGDREWLRESGYPLMKGAAEFALDWLVEGPDGHLVTA
                     PSTSPENVYRTPDGYEGSVSIMTTSDLALVRGLFLRVIEAAADLGADSDFRARVAAAL
                     ERLPPYKIGRRGHLQEWYEDWEDRDPQHRHVSHLIGLFPDDQITPSDTPALAQAARRS
                     LELRGDGGTGWSKAWKIALWARLLDGDRAYGMLRSHLHYVPATGEERHTGGGTYPNLF
                     DAHPPFQIDGNFGGTAGIAEMLLQSRRGALHLLPALPAAWPSGSVRGLRARGGFTVDI
                     RWKDGALESATIRADRDGPARVRYGRAAVERRFAAGRPVTLVSGPDGLTAR"
     gene            complement(2387..3832)
                     /locus_tag="PLT35_05900"
     CDS             complement(2387..3832)
                     /locus_tag="PLT35_05900"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG28797.1"
                     /translation="MPGVTRLAITGAVVAVAACCGLAAAARGQQESASPPAWAQAVDA
                     VFGEWDRPGSPGCAVAVSRDGAVAYARGYGLADLEHDAPITPDSVFYAGSVSKQFTAM
                     AAALAMGQGRLGPDDDVRTYVPELPDYGRTMTIRHLLHHASGLRDVNTLLVLAGRRDD
                     EAFDNEGVLRIVARQRALNFPPGDGHDYSNSGYALLALAVERATSTPFPAFADAHIFA
                     PLGMTATHFHTDVARVVKHRAWAYDRRADGTFALNSPQNERAGAGGLFTTVGDLARWD
                     ENFYSGRVGGPATIRLLESPGRLNDGTVLAYAWGLRIASYRGAPVVEHSGSLGGYRAH
                     VFRVRGARTSVSLLCNVSGVDTGAAVRRVADAVIGGWFTEKPASAPGPAAASEPPGPA
                     RAYSASELAALAGTYCSDELESCYRVSVNDGRLELRRGTARAVLRPRAGPEGEFRLPD
                     SSIRFTRSADGSVAGLLVESGRIRGLAFSKR"
     gene            complement(3862..5319)
                     /locus_tag="PLT35_05905"
     CDS             complement(3862..5319)
                     /locus_tag="PLT35_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOG28798.1"
                     /translation="MSADPSRLSSRLRELLRNAPPRSGGRADAAEAAGPRQPSAPGRG
                     LVYAPEDAPSGRGGAGGAGCAVIERQYAGERLHGRRRVAEYGEMLARCMPGLAVLAAD
                     DRAAAPREPRRERLQWDGGARLDESRRRAGAPSGGPLLFFDLETTGLSGGAGTLAFLA
                     GCGSFDQDGFHTRQLFLSACESERELLDGLAGMAGRYGGLVTFNGRAFDVPLIETRYA
                     FHRLESPFSGLPHFDLLHPARRLWRRRGLPGPSHRDRRAGGRPGDAASCALGALEEAI
                     LGLARLDDVPGAEIPSRYFGYLRTGDLGPLEPVFEHNRLDLLSLAALTAIAAQMAVEG
                     PSGTASPHEALAMGRIYQQLGREDEADACFSRASGLGGAPWEADAVDAGVRADALRAL
                     ARGRRRRRRYAEAADAWERLLASGASLAFAQEAREALAIHHEHRARNLAAARELAVRA
                     LAAERDPGAAAGLRRRLARIDRKIGASGARRGGGD"
     gene            complement(5510..>6157)
                     /locus_tag="PLT35_05910"
     CDS             complement(5510..>6157)
                     /locus_tag="PLT35_05910"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOG28799.1"
                     /translation="TITYSRVTPLDTAGAAGERPRALAAHGDVHVVSRVVGFKKIKFY
                     TNENVGSGELDLPERQMHTTSYWLTVPRELVGSLSWPGDDRRDGVVGMAYAMRQVAQL
                     LLMCDARDIGVSIGSARDAAEDGSAQPAAGPPSGVPPGARDGDEPRVFIYDNYPGGIG
                     FSAPLFRMHRDLLARTRELIAGCPCESGCPSCVGPLGDVGPKAKEVALELLGRLQ"
BASE COUNT          843 a         2265 c         2245 g          804 t
ORIGIN      
        1 catctcggcc agtgtggaca gcccgacgtc atcggtgagc gccaccagcc gagccaggcg
       61 ctcccgctcg gcgacggccg gacgggggtc atggtgttcg gcgggccctc ctccagccgc
      121 ttcctgctga acgactcgac gctgtggtcc ggaggcccgg tcgaccccgc gatgaacccg
      181 gacgcgatcg cctggctgcc caaggttcgc gaggcgctgt tcgcgggcga ctaccggctg
      241 gccaccgagc tgacgcggaa gcttcagggg cgcttctccg agtcgtacgc gccgctcggc
      301 gacctctacc tcgacatgcc ttccgttgcc gccggctccg tctcggccta tcgccgcgag
      361 ctcgacatcg ccgcgggcgt ggccaggacg agcttcaagg ccgggctgac ggcgatcgcg
      421 caggaggcgt tcgtgtccca ccccgcgcga accctgctcg tccggctcac ggcgtccgcg
      481 cccggcgcac ttgccttctc gctccggttc gagagccagc tgaagcacgc ggcggcggtc
      541 gatgcgagcg gcgatctgct gatgacggga cgcgcgcccg tgcgggccga gccgaactac
      601 cgcggcaaca tcaaggaccc gattgtctac gacgaggggc ccgatcccaa ggggatgcgg
      661 ttcgcggccc gggcgagggt gctccgcacg gacggccgga tcgagcgggg accttcgtcg
      721 ctcgccgtcg ccggcgcgcg cgaggcgctt gtcgcgatcg cgctggacac gagcttcgcg
      781 gggttcgacc gggagccgtc cggcggcccg gatcccgttc cggccacgcg cgccacgctc
      841 gacgcggccg ccaggcagcc ttatgccgcc ctgcgactgg cgcacgcacg cgacgtcgag
      901 gcgcttatgt cccgcgtcgc gctggacctc gggcgcccgg cgagagacga gatccccacc
      961 gacgtccgcc tgcgcgagta cgcggacggc gccggggacc ccggcctcga ggcgctctac
     1021 ttccagttcg gccgctacct gctcgtcgcc gggtcgcgtc cgggctcgcc ggcgctcaac
     1081 ctgcagggca tctggaaccc tcacatgcgc ccgccctgga gcagcaacta cacgatcaac
     1141 atcaacacgg agatgaacta ctggccggcg gagacgacga acctcgcgga gtgccacggc
     1201 ccgctcctgc ggttcatcgg ggatgtcgcc aggacgggcg cggtgacggc gcgccactat
     1261 tacggcgccg gcggctgggc ggcccaccac aacacggacc tctgggcgat gagcaatccg
     1321 gttggcgact tcggccaggg tgacccggtg tgggcgaact ggccgatggg cggcgtgtgg
     1381 ctcgcccggc acctgtggga gcattacgcg ttctcgggag accgggagtg gctgcgcgag
     1441 tccggctacc cgctcatgaa gggcgccgcc gagttcgccc tcgactggct ggtcgaaggg
     1501 ccggacgggc acctcgtcac ggcgccgtcc acgtcgccgg agaacgtgta caggacgccg
     1561 gacggctacg aaggctcggt gtcgatcatg acgacgagcg atctcgccct ggtgcggggg
     1621 ctgttcctgc gcgtcatcga ggcggccgcc gatctcggcg ccgactcgga cttccgcgcg
     1681 cgcgtcgcgg cggcgctcga gcggctgccg ccgtacaaga tcggccgccg cggccatctg
     1741 caggaatggt acgaggactg ggaggatcgg gatccgcagc accggcacgt gtcccatctc
     1801 attgggctct tccccgacga tcagatcacg ccctccgaca cgccggcgct ggcgcaggcg
     1861 gcgcgccggt cgctggaact gcgcggcgac ggcggcacgg gctggagcaa ggcctggaag
     1921 atcgcgttgt gggcgcgcct tctcgacggc gaccgcgcat acggcatgct gcgctcgcac
     1981 ctgcactacg tgccggcgac gggcgaagag cggcacacgg gcggcggcac gtacccgaac
     2041 ctcttcgacg cgcatccgcc gtttcagatc gacggcaact tcggcggaac ggccggcatc
     2101 gcggagatgc tgctgcagag ccggcgcggc gcgcttcacc tgctgcccgc gctgccggcc
     2161 gcgtggccgt cgggctcggt tcgcggcttg cgcgctcgcg gcgggttcac ggtggacatc
     2221 cgctggaagg acggcgccct cgagtcggcg acgatccgcg cggatcgcga cggccccgca
     2281 cgcgtgcggt acggcagggc cgcagtcgaa cgccggttcg ccgcgggcag gccggtgacc
     2341 ctcgtctccg gccccgacgg actgacggcc cggtagcggg cccgggtcag cgcttcgaga
     2401 aggcgagccc ccggatgcgg ccggactcga cgaggaggcc ggcgacggat ccgtccgcgc
     2461 tgcgcgtgaa acgaatcgag ctgtcgggga ggcggaactc cccttccggc ccggctctcg
     2521 gcctcaggac tgcgcgcgcc gtgccccggc gcagctccag ccggccgtcg ttgacggaca
     2581 cgcggtagca gctctctagc tcgtcgctgc agtaggtgcc ggcaagcgcc gccagctccg
     2641 acgcgctgta ggcgcgcgcg gggcccggcg gttcggaagc tgccgcgggc cccggagcgg
     2701 aagccggctt ctcggtgaac cagccgccga tcacggcgtc ggcgacacgg cgaaccgcgg
     2761 ccccggtgtc cacgccggac acgttgcaca gcaacgagac cgacgtgcgg gcgccccgga
     2821 cccggaagac gtgggcgcgg tacccgccaa gcgagccgct gtgctccacg acgggcgcgc
     2881 cgcgatagga ggcgatcctc aggccccagg cgtaggccag cacggtgccg tcgttcaggc
     2941 gccccggtga ttcgagcagc cggatcgtgg cggggccgcc gacgcggccg ctgtagaagt
     3001 tctcgtccca gcgcgccagg tcaccgaccg tcgtgaacag cccgccggcg ccggcgcgct
     3061 cgttctgcgg cgagttgagc gcgaacgtcc cgtcggcgcg gcgatcgtac gcccaggccc
     3121 ggtgcttcac gacgcgcgcg acgtcggtgt ggaagtgcgt ggccgtcatg ccgaggggcg
     3181 cgaagatgtg cgcgtcggca aacgccggga acggcgtgga cgtcgcccgc tcgacggcca
     3241 gggccagcag ggcgtagccc gaattggagt agtcgtggcc gtcgccgggc gggaagttga
     3301 gcgcgcgctg ccgcgcgacg atccgcagga ccccctcgtt gtcgaacgcc tcgtcgtcgc
     3361 ggcggccggc gagcacgagg agcgtgttga cgtcgcggag cccgctcgcg tgatgaagga
     3421 ggtgccggat ggtcatcgtc cggccgtagt ccggcagctc cggcacgtac gtccgcacgt
     3481 cgtcgtccgg cccgaggcgg ccctgcccca tggcgagcgc cgcggccatc gccgtgaact
     3541 gcttggacac ggaccccgcg tagaacacgg agtccggcgt gatgggcgca tcgtgctcga
     3601 gatcggccag gccgtagccg cgcgcgtagg ccacggcccc gtcccgggac accgccacgg
     3661 cgcagcccgg cgatccgggg cgatcccact cgccgaacac ggcgtcgacg gcctgcgccc
     3721 aggcgggcgg cgaggcggac tcctgctggc cgcgcgcggc cgcggccagc ccgcagcatg
     3781 ccgccacggc tacgacagcc ccggtgatcg ccagtcgcgt gacgcctggc atctggacct
     3841 cctactctga cggccgccag cctagtcccc gccgccccgc cgcgctccgg acgcgccgat
     3901 cttcctgtcg attctggcta ggcggcgcct gaggccggcg gccgcgccgg ggtcgcgctc
     3961 agccgccagg gcgcgcacgg ccagttcccg cgcggcggcc agattccggg cgcggtgctc
     4021 gtggtggatg gccagcgcct cgcgagcttc ctgcgcgaag gcgaggctgg cgcccgatgc
     4081 gagcaggcgc tcccaggcat cggcggcttc ggcgtagcgc cgtcggcgcc tgcgcccgcg
     4141 cgcaagggcc cgcagggcgt cggccctgac ccccgcgtcg actgcgtccg cctcccacgg
     4201 cgcgccgccc aggcccgagg cgcgcgagaa gcaggcgtcc gcttcgtcct cgcgtccgag
     4261 ctgctggtag atccggccca tggcgagggc ctcgtgggga gacgcggtgc cggacggccc
     4321 ttcgactgcc atctgcgccg caatcgcggt cagcgcggcc agcgagagca agtcgaggcg
     4381 attgtgctcg aagacgggct ccagcggccc gaggtcaccc gtccggaggt agccgaagta
     4441 gcgcgagggg atttccgcgc ccggcacatc gtcgaggcgc gcgagcccga ggatggcctc
     4501 ctcgagcgcg cccagcgcac agctggcggc gtcgccagga cggccgccgg cccggcgatc
     4561 gcgatgggac ggcccgggca ggccccgccg gcgccagagc cttctggccg ggtgcagcag
     4621 atcgaagtgc ggcaggcccg aaaacggcga ctcgaggcgg tggaacgcgt agcgggtctc
     4681 gatgaggggc acgtcgaacg cgcggccgtt gaaggtcacg agcccgccgt atcggcccgc
     4741 catgccggcc agcccgtcga gcagctcccg ttcgctctcg caggcgctca ggaagagctg
     4801 gcgcgtgtgg aacccgtcct ggtcgaagga cccgcagccc gcgagaaagg ccagcgtgcc
     4861 tgcccctccg ctgaggccgg tggtctcgag gtcgaagaac agcagcggcc cgccggaggg
     4921 ggcgcccgcg cgccgccggg actcgtcaag ccgcgctccg ccgtcccact gcaggcgctc
     4981 gcggcgcggc tcgcgcggcg cggccgcacg gtcgtcggcc gccagcaccg ccagccctgg
     5041 catgcaccgc gccagcatct ccccgtactc ggccacgcgg cgcctgccgt gcaggcgttc
     5101 tccggcgtac tggcgctcga tgaccgcgca gcctgcgccg ccagcgcctc cgcggccgct
     5161 cggcgcgtct tcgggcgcgt aaactaggcc gcgccccggt gctgacggct gccgcggccc
     5221 ggccgcctcc gccgcatcgg cgcgcccccc ggacctggga ggcgcgttgc gcagcagctc
     5281 gcggaggcgc gacgagaggc gggacggatc ggcggacatc gtggatcaga ggaagacgga
     5341 atccagcagc cagaagccag cagtcagaag ccagaatcgg gacatgccga gccggggcgc
     5401 gccgttcgtt ggatgtcccg gctccgccct ccactcctgg tctccggcgg catctcccga
     5461 cgccggctcc attccgaatt ccggattctg catgaaaccc gatttcctgc tactgcagcc
     5521 gcccgagcag ctccagcgcc acctctttcg ccttcggccc gacgtccccg agcggcccga
     5581 cgcacgacgg acagcccgac tcacaggggc agccggcgat gagctcgcgc gttcgcgcca
     5641 gcaggtcccg gtgcatgcga aacagcggcg cgctgaaccc gatgccgccg ggatagttgt
     5701 catagatgaa cacgcgcggc tcgtcgccgt cgcgggcgcc cgggggcacg cccgatggcg
     5761 gcccggccgc cggctgcgcc gagccgtctt cggcggcgtc tctcgcggac ccgatggaca
     5821 cgccgatgtc gcgcgcgtcg cacataagca gcagctgagc aacctgccgc atcgcgtacg
     5881 ccatcccgac gacgccgtca cggcggtcgt cgccgggcca cgagagcgac ccgacgagct
     5941 cgcgcggcac ggtcagccag tacgacgtcg tgtgcatctg gcgctcgggc agatcgagct
     6001 cgccggaccc gacgttctcg ttcgtgtaga acttgatctt cttgaagccg accacccggg
     6061 acacgacgtg aacgtctccg tgggccgcca gcgcccgcgg ccgctcgccg gcggcgccgg
     6121 ccgtgtcgag cggcgtgacg cggctgtagg tgatggt
//
LOCUS       DAOSIF010000133         6112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11644_length_6112_cov_3.781410, whole genome shotgun sequence.
ACCESSION   DAOSIF010000133 DAOSIF010000000
VERSION     DAOSIF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11644_length_6112_cov_3.781410"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLT35_05915"
     CDS             complement(<1..383)
                     /locus_tag="PLT35_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG28800.1"
                     /translation="MPGDGVGVDVMEAARIVLERADFDAEYLHGDIGWEFWCAEGDAL
                     PPRTIELLRHVDAALFGAITSKPVKAAEAELAPSLKGTGLIYRSPIVRMRQTFDLYTC
                     LRPMKAYPGNALNHDDGVDLVMFRE"
     gene            768..1217
                     /locus_tag="PLT35_05920"
     CDS             768..1217
                     /locus_tag="PLT35_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG28801.1"
                     /translation="MTLVRWDPFRELSAMQERMNRLFGEAYRPGEDDVMRRGAWVPPV
                     DIYDAGNHELVIKAELPDMTKDDIEIVVANNSLTLRGEKKMDSSIKEESCHRIERTYG
                     AFSRSFSLPPTVDASKVSADYKNGVLTIKLPVREDAKPKQIQVRVQD"
     gene            1455..1700
                     /locus_tag="PLT35_05925"
     CDS             1455..1700
                     /locus_tag="PLT35_05925"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOG28802.1"
                     /translation="MLNPTEARFLFVEVAAQRAKQLRRGALSRLRPADADPGRAPESP
                     APARKPERVAMDEVRQGYIQYDFADAPPSHPKLEGLL"
     gene            1697..1948
                     /locus_tag="PLT35_05930"
     CDS             1697..1948
                     /locus_tag="PLT35_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28803.1"
                     /translation="MSVNEVGALVAAALQSVGGAIATILVLMIGLSLGLDVFKLRPSG
                     GRSLTVRSLDEALGKPQTYLPPDIPRGTTDQLGNSRRAG"
     gene            2063..2377
                     /locus_tag="PLT35_05935"
     CDS             2063..2377
                     /locus_tag="PLT35_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28804.1"
                     /translation="MATMLTLAAMAVFGLFVLGLVGGLLKLLFWLLVLPFRLLGLVLK
                     LLFGLLLLPLFLALAVVGAAGFGLLALLGLLLPFLPLLVLGLAVWGLAKLIARPAVAS
                     GA"
     gene            complement(2366..3823)
                     /gene="gatA"
                     /locus_tag="PLT35_05940"
     CDS             complement(2366..3823)
                     /gene="gatA"
                     /locus_tag="PLT35_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOG28805.1"
                     /translation="MSGEALTAAGIRAAFVNGEATASAICRAALDCIAERNGPLGAVL
                     RVDEARAMARAAVLDRAAGTSGAGPLAAVPVLVKDNICVEGGVTTAGSRLLERFVAPY
                     TATAVERLERSGAIVIGKTNCDEFAMGSSNEHSAFGPARNPWDLSRTPGGSSGGSAAA
                     VAARMAPLALGSDTGGSVRQPAGFCGCVGLRPTYGRVSRYGLVAFSSSLDQIGPIALT
                     VRDAAAALGAMAGLDPQDATTSDAPVPDWAGAMTGDVRGCRLGVPVRLLDGIDDGVRA
                     AFGAALEVLHSRGALIRDVSLEHASLAIAVYYVLATAEASSNLARYDGVRYGYRAAGA
                     ADLRGMIERTRTEGFGPEATRRILLGTFVLSAGHYDAYYAKAQRVRALVAADFADAFR
                     DVDVIALPTSPTPAFRLGELVDDPLSMYLADVFTVGASLARLPALSVPCGFTPAGLPV
                     GLQLAGRPFDESTILRVAEAYERDTAFWRVAPSGA"
     gene            complement(3820..4137)
                     /locus_tag="PLT35_05945"
     CDS             complement(3820..4137)
                     /locus_tag="PLT35_05945"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOG28806.1"
                     /translation="MLASAMAGARDDFDIARVAALAGLELAPADAARLHAQLASIVGY
                     VGQLQDADTSGGAPGQADQPAADALRPDDVRASLPADAVLMNAPDATADPPLVRVPRV
                     MLR"
     gene            4146..4988
                     /gene="amrB"
                     /locus_tag="PLT35_05950"
     CDS             4146..4988
                     /gene="amrB"
                     /locus_tag="PLT35_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG28807.1"
                     /translation="MAPQPVRRPAAVAGTWYPGQPARLAAEIERYTEAVSARISGDIV
                     GLVAPHAGLAYSGPVAAHAYRQLDGRAYDVVVLVGPSHHVGFEGVAIAPRGVFETPLG
                     DLPISEADADAIMAASPIVQDLPAAHRREHSVEMQLPFIQRMLPGVPIVPLVMGHQSR
                     STVAALGDGLAAALAHRRPLLVASSDLSHYQDSRRAAQLDQVIVTAVERFDPEAVERA
                     LARNPEHACGGGPMVAVMRAARALGAADARVLCYADSGAVSGDTSAVVGYMAAVLGTF
                     RAAA"
     gene            5007..5567
                     /gene="amrA"
                     /locus_tag="PLT35_05955"
     CDS             5007..5567
                     /gene="amrA"
                     /locus_tag="PLT35_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOG28808.1"
                     /translation="MAARLTDEDRRRLLAIARRAVADALAGRPRAAVDTTGALGARAS
                     AFVSFHSGGALRGCIGRLGATQPLAAVVADCAVAAATDDPRFDPVGAGELDGCVIEIS
                     VLGPVSPMDDPEDIVVGRHGLIVEQGRQRGLLLPQVAVEQRWDRDTFLERTCGKAGLA
                     PDAWRRGARVSLFEADIFDDAAPGAR"
     gene            5586..>6112
                     /locus_tag="PLT35_05960"
     CDS             5586..>6112
                     /locus_tag="PLT35_05960"
                     /inference="COORDINATES: protein motif:HMM:NF021676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28809.1"
                     /translation="MEIYLLPIPRGRHELYCEFDERQPPAGADGRSRWRRWLSRRFQD
                     GLASLDAERRKRLARASAAPARTRLQRIRDRAVAWIAERVAEQRLLWILRGQTAVTLH
                     YPDDDDEAGADRIVRSSLQHDGRYHFALMIVDGLLYLAALPLTPLPGPNVPSIYFSFR
                     AIGHLLSWLGARRGLR"
BASE COUNT          855 a         2228 c         2201 g          828 t
ORIGIN      
        1 ttctcgcgga acatcacgag atcgacccca tcgtcgtggt tcagcgcgtt gccggggtag
       61 gccttcatcg gccggaggca ggtgtacaga tcgaaggtct gccgcatgcg gacgatgggg
      121 gagcggtaga tgaggccggt ccctttgagc gagggcgcca gctccgcctc cgccgccttg
      181 accggcttgg acgtgatcgc gccaaacagc gccgcgtcca cgtggcggag cagctcgatc
      241 gtgcgcggcg gcagcgcgtc gccctcggcg caccagaact cccagccgat gtcgccgtgc
      301 aggtactcgg cgtcaaaatc cgcccgttcg aggacgatcc tcgcggcctc catcacatcg
      361 acgcccacac cgtccccggg caaccacgca atcctgtaac gcggcatacg caccaccctc
      421 ggcaagggga gacaacggca gacccggcag cccccgatcc ggcacggcct tgcggggctt
      481 cgtccgacct gccgcgccag tgaggcctcg acaggatacc aagactcctg gagaacagcc
      541 agcctgctcg acgagcccgc gttgcgaccg tgtgcgagcg ggcgctcggt cgccagcctg
      601 gcgccggagt ctccagcaag gcgaaaagtg cttgatataa taagactcag ataattttag
      661 ttgacaacag tgatgcatcg tgtaaaataa ctctagattg caacgcggac ggctctggag
      721 tgaccgggcc ggcccgccaa gcggattcaa cgatgaggag gaaggccatg acactcgttc
      781 gctgggaccc gttccgtgag ctgagcgcga tgcaggagcg catgaaccgc ctgttcgggg
      841 aggcgtaccg ccccggcgag gacgatgtga tgcgccgcgg cgcgtgggtg ccgccggtgg
      901 acatctacga cgccggcaac cacgagctgg tcatcaaggc cgagctgccc gacatgacca
      961 aggacgacat cgagatcgtc gtggcgaaca acagcctcac cctgcgcggt gagaagaaga
     1021 tggacagctc catcaaggaa gagagctgcc accggatcga gcgcacctac ggcgccttca
     1081 gccggtcgtt ctccctgccg ccgacggtgg acgcgtcgaa ggtcagcgcc gactacaaga
     1141 acggcgtgct gaccatcaag ctgccggtgc gcgaggacgc gaagccgaag cagatccagg
     1201 tccgcgtgca ggactaggcc gggccgccgg ctgcggcgcg gccgccacca gctccggggg
     1261 cgcacgcggg cgcccccgga cgctgtacga acagcacgcc ccgcccgcgt gcgcgggagg
     1321 cgccggcggc cgcggaaagg cttgccagac ccttcacgcg tggtgtactt ttacctgttg
     1381 tggatcaagc accgattgtc gaacccgtcg ccctcctcga gacccccgaa aagccagagc
     1441 ccgacgagcg cttcatgctg aacccgaccg aggcgcgttt cctgttcgtc gaagtcgccg
     1501 cgcagcgggc gaagcagctc cgccgcgggg cgctcagccg cctgcggccc gccgatgctg
     1561 atcccgggag ggcgccggaa tcgccggcac ccgcgcgcaa gcccgagcgc gtcgcgatgg
     1621 acgaagtccg ccagggctac atccagtacg atttcgcgga cgcgccgccc tcgcacccga
     1681 agctcgaggg gctgctgtga gcgtgaacga ggtcggcgcc ctggttgccg ccgccctgca
     1741 gtcggtgggc ggcgccatcg cgacgatcct cgtgctgatg atcgggttgt cgctcggcct
     1801 ggacgtcttc aagctgcgcc ccagcggcgg ccgatcgctg accgtgcgca gcctggacga
     1861 ggccctgggc aaaccccaga cctacctccc gccggacatc ccgcgcggga cgaccgacca
     1921 gctcggcaac tcccgccgcg ccggctgacc cggcacgccg gtccgctgga acctcgaccc
     1981 gcccattcct cgtagatcgg tataacgggc gcggcaagcc gcggcgggcc cgcccgcaag
     2041 gcgcgagagg ggagagcaca ccatggccac gatgctgacg ctcgccgcga tggccgtctt
     2101 cggcctgttc gtccttggac tggtgggcgg cctcctgaag ctcctcttct ggctgctcgt
     2161 cctgccgttc aggctgctcg gcctcgtgct gaagctgctt ttcggcctcc tgctcctgcc
     2221 gctcttcctg gcgctggccg tggtcggggc ggcgggcttc ggcctcctgg cgctcctcgg
     2281 gctgctgctg ccgttcctgc cgctgctcgt gctggggctg gccgtgtggg ggctggcgaa
     2341 gctgatcgcg aggcccgccg tcgcgtcagg cgcctgacgg cgcgacgcgc cagaacgccg
     2401 tgtcccgctc gtaggcctcg gccacccgca ggatcgtcga ttcgtcgaag gggcgccctg
     2461 cgagctgcag gccgacgggc agcccggcgg gggtgaagcc gcagggcaca gagagcgcgg
     2521 gaaggcgcgc gagcgacgcg ccgacggtga agacgtccgc caggtacatc gagagcgggt
     2581 cgtccaccag ttcgcccagc cggaacgccg gcgtcgggct ggtcggcagc gcaatgacat
     2641 ccacgtccct gaacgcgtcc gcgaaatccg ctgcgacgag cgcgcggacc cgctgggcct
     2701 tcgcgtagta cgcgtcgtag tgccccgcgc tgagcacgaa ggtcccgagg aggatgcggc
     2761 gcgtggcctc ggggccgaag ccctcagtcc tcgtccgctc gatcattccg cgcaggtccg
     2821 cggcacccgc cgcgcggtac ccgtatcgca cgccgtcgta ccgtgcgagg ttcgaactcg
     2881 cctcggccgt cgccaggacg tagtacaccg caatggcgag gctggcgtgt tcgagggaca
     2941 cgtcccggat cagggcgccg cgcgaatgca gcacctcaag cgccgcgccg aatgcggcgc
     3001 gcacgccgtc atcgatgccg tccagcaggc ggaccggcac gccgaggcgg catccgcgca
     3061 cgtcccccgt catcgcgccc gcccagtcgg gaacgggcgc gtcggacgtc gtcgcgtcct
     3121 gcgggtcgag gcccgccatc gccccgaggg cggcggcggc gtcgcggacg gtcagggcga
     3181 tgggcccgat ctggtcgagc gacgacgaga aggcgaccag gccgtagcgc gacacgcggc
     3241 cgtaggtggg gcggaggccc acgcagccgc agaagccggc cggctggcgc acggagcctc
     3301 ccgtgtccga gccgagggcg agcggcgcca tccgggccgc gacggcggcc gccgacccgc
     3361 cgctcgatcc gccgggcgtc cgcgacaggt cccaggggtt gcgcgcgggg ccgaacgccg
     3421 agtgctcgtt cgacgagccc atcgcgaact cgtcgcagtt cgtcttgccg ataacgatgg
     3481 cgccggagcg ttcgaggcgc tccacggcgg tggccgtgta gggggccacg aagcgctcga
     3541 gcaggcgcga cccggccgtc gtcaccccgc cctcgacgca gatgttgtcc ttcacgagga
     3601 cgggaaccgc ggccaggggg ccggcgccgg acgtcccggc cgcgcgatcg agcacggcgg
     3661 cgcgcgccat cgcccgcgcc tcgtccaccc gcaggaccgc gcccagcggc ccgttgcgct
     3721 ccgcgatgca gtcgagcgcc gcgcggcaga tggccgatgc cgtcgcctcg ccgttcacga
     3781 acgccgcgcg gatcccggcc gccgtcagcg cctcgccgct catcgcagca tcactctcgg
     3841 aacgcggacc agggggggat cggccgtcgc gtcgggcgcg ttcatcagga ccgcgtcggc
     3901 tggcagcgac gcccgcacgt cgtccgggcg gagggcatcg gccgcgggct gatcggcctg
     3961 gccgggcgcg ccgccgctcg tgtccgcgtc ctggagctgt ccgacgtagc cgacaatcga
     4021 ggcgagctgg gcgtgaaggc gggccgcgtc ggccggggcc agttcgagcc cggccagggc
     4081 ggcgacgcgg gcgatgtcga agtcgtcgcg tgcgccggcc atggccgatg ctaacattca
     4141 cgatcatggc accgcaacca gtccgcaggc ccgccgcggt ggccggcacc tggtatccgg
     4201 ggcagccggc gcgcctcgcg gcggagatcg agcgctacac ggaggccgtg tccgcgcgca
     4261 tcagcggcga catcgtcggc ctggtggcgc cgcacgccgg cctcgcctac tctgggccgg
     4321 tggcggcgca cgcgtaccgc cagctggacg gccgcgccta cgacgtcgtg gtgctcgtcg
     4381 ggccgtccca ccacgtcggg ttcgaaggcg tggccatcgc gccgcgcggg gtcttcgaga
     4441 cgccgctcgg cgatctgccc atctccgagg cggatgccga cgcgatcatg gcggcgtcgc
     4501 cgatcgtgca ggacctgccg gcggcgcacc ggcgggagca ctccgtcgag atgcagctgc
     4561 ccttcatcca gcgcatgctg ccgggcgtcc ccatcgtgcc gctcgtgatg ggccaccagt
     4621 cgcgctcgac ggtggccgcc ctcggcgacg ggctggccgc cgccctggcg caccggcgcc
     4681 cgctgctcgt cgcaagcagc gatctctccc actaccagga ctcgaggcgc gcggcgcagc
     4741 tggaccaggt gatcgtcacc gccgtcgagc ggttcgatcc cgaagcggtc gagcgggcgc
     4801 tggcgcgcaa ccccgagcac gcctgcggcg gcggcccgat ggtcgccgtc atgcgcgcgg
     4861 cccgcgccct cggggcggcc gacgcccggg tgctgtgcta cgcggactcc ggcgccgtgt
     4921 cgggcgacac gagcgcggtc gtcggctaca tggccgccgt cctcggaacg ttcagggcgg
     4981 cggcctgatc gggccgggtg ggcgcgatgg ccgcgaggct gaccgacgag gatcggcgcc
     5041 gcctgctggc gatcgcgagg cgggccgtcg ccgacgccct ggccgggcgg ccgcgcgccg
     5101 ccgtcgacac gacgggcgcg ctcggcgcgc gcgcgagcgc cttcgtgtcc tttcacagcg
     5161 gcggcgcgct ccgcggctgc atcgggcgcc tcggcgccac ccagcccctg gccgccgtcg
     5221 tcgccgactg cgccgtggcg gccgccaccg acgacccgcg gttcgacccg gtgggcgcag
     5281 gcgaactgga cggctgcgtg atcgagatct ccgtgctcgg ccccgtctcg cccatggacg
     5341 accctgagga catcgtcgtc ggccggcacg ggctgatcgt cgagcaggga cggcagcggg
     5401 gcctgctgct gccgcaggtc gcggtcgagc agcgctggga ccgcgatacg ttcctcgagc
     5461 gtacctgcgg caaggcgggg ctcgcgcctg acgcgtggag gcgcggcgcg cgcgtctcgc
     5521 tgttcgaggc ggacatcttc gacgatgccg ccccgggcgc ccgctaggcg ggctggtgcg
     5581 gctccatgga gatctacctc ctgcccatcc cccggggtcg ccacgagctc tactgcgagt
     5641 tcgacgagcg ccagccgccg gccggcgccg acggccggtc gcgctggagg cgctggctca
     5701 gccggcggtt ccaggacggc ctggccagcc tcgacgccga gcgccggaag cggctggctc
     5761 gcgcctcggc cgcaccagcg cggacgcggc tgcagcggat ccgggaccgc gccgtcgcgt
     5821 ggatcgccga gcgcgtggcg gagcagcggc tcctgtggat cctgcgcggc cagacggctg
     5881 tgaccctgca ttatccggac gacgacgacg aggcgggcgc agaccgcatc gtgcgcagca
     5941 gcctgcagca cgacgggcgg tatcacttcg cgctgatgat cgtcgacggc ctgctctacc
     6001 tcgccgccct gccgctgacg ccgctccccg gcccgaacgt gccctcgatc tacttctcct
     6061 tccgcgcgat cggccacctg ctctcgtggc tcggtgcgcg gcggggcctt cg
//
LOCUS       DAOSIF010000134         6108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11653_length_6108_cov_3.610606, whole genome shotgun sequence.
ACCESSION   DAOSIF010000134 DAOSIF010000000
VERSION     DAOSIF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11653_length_6108_cov_3.610606"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..588)
                     /locus_tag="PLT35_05965"
     CDS             complement(28..588)
                     /locus_tag="PLT35_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOG28810.1"
                     /translation="MPMDAAAVAAVLERLVPGSGAQALAAIDQPTLVVSADRLAEVCL
                     ALRDDPALGYTALLDIVGVDRQGAEPRFELSYSLLAADAPARLRVKVRLAGPEPHVGT
                     VTGIWPSAGFLEREVWDLLGVVFDGHPDLRRLLTPDEWEGHPLRKDYPVQIGAPVRTY
                     EPIQVTEEQFAERILKSRRVRGGGTR"
     gene            complement(588..1073)
                     /locus_tag="PLT35_05970"
     CDS             complement(588..1073)
                     /locus_tag="PLT35_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOG28811.1"
                     /translation="MAPEWDIPILTTTVDKMVQWARRSSIWPVTLGLACCAIEMMSMA
                     ASRYDIARFGAEVFRASPRQADLMIVAGRLSRKMAPAVRRIYDQMPEPKWVISMGACA
                     SCGGVFDNYAIVQGCDQVVPVDVFVPGCPPRPEALLYAIVALQRKIDRQRAFAYPAAR
                     P"
     gene            complement(1122..1481)
                     /locus_tag="PLT35_05975"
     CDS             complement(1122..1481)
                     /locus_tag="PLT35_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOG28812.1"
                     /translation="MQGWLSIFLLAALGLGFAAVSIAASRALGPRHRTPEKDAPYECG
                     MPPVGDARERHSVKFYLVAMIFLLFDIEVAFLYPWAMAIRDLRWVGFIQVLVFFLVLA
                     AGYVYVWKKGAIDWNRP"
     gene            1591..2976
                     /locus_tag="PLT35_05980"
     CDS             1591..2976
                     /locus_tag="PLT35_05980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG28813.1"
                     /translation="MRIAWFSPLPPDRSGIAAYSADVLAGLRGHEIDAFIDDTSGQDA
                     VLRARPLAGASIRGAHEFVWRRARRPYDVVVYQLGNSAAHDYLWPYLVRYPGLVVLHD
                     AQLHQSRARALIRQFREDDYKAEFRYSYPGVPPLLADLVVAGLGATLCYFWPMVQIPV
                     ESARTVAVHNAHLASALRGRFPGCDIVRIHMGVPDPGVPPGAAAGVRRRHGIPEDAVV
                     FGSFGRVTPEKGLTAVVRALAHVAPSVPNLRLLTVGEPVGYYNLVAQARELGVADVVV
                     SAGYVGDGELASYLAATDVCLNLRWPTGRETSAAWLRCVAAGKPTIVTDLAHQTDVPS
                     LDVRTMAAAPGGPAGAEPVCLHIELVDEIPMLRLALQRLCGDAGLRERLGRAARRYWE
                     EHATVGLMVRDYEGLLARAAAAPDPPRPPAWPAHLAADGSALARALAAEAGVPYPLAA
                     GPGGPESSGRA"
     gene            2994..4487
                     /locus_tag="PLT35_05985"
     CDS             2994..4487
                     /locus_tag="PLT35_05985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG28814.1"
                     /translation="MFAEDDIERRRVEREAADRAYNEALTALDQAFPRMPELPHPPVV
                     YDESRLPAINERWEIVPADPAPGASGWRRRAARLVWSIVRPAFERQQAFNSALVDHLN
                     RNVAVHRANREAIAAAIETLRGELEKLQAFHSRLVMYLQTITRFVDTKDRSEHIARLA
                     NITQAGLSGVVDEFRRDWEAMTARDQRHVAAVDELRTSIAAVQQTGLTLKRELERLLA
                     GRAAPAEAGAAAAGPAQPGAEPTGGAQQTKEARPAPGAGAVPPAADAVNAYKYVGFEN
                     KFRGSEPEIRARLEDYVGLFAGASDVLDLGCGRGEFLALLAERGIAGRGLDINHEMVE
                     LCRARGLEAREGDALAYLESLADGALGGLIAVQVVEHFQPAYLVRLLDAAFHKLRPGS
                     RIVLETLNPACWFAFFDAYIRDITHAWPLHPETLKYLVVASGFQRADVRMLSPYPADA
                     RLQPLWLPPGADTLAMTTMAQVFNENMAKLNAVMFSELDYAVTAERM"
     gene            complement(4675..5970)
                     /locus_tag="PLT35_05990"
     CDS             complement(4675..5970)
                     /locus_tag="PLT35_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOG28815.1"
                     /translation="MTLLERVQRKDCNVGVIGLGYVGLPLAVELALGGFRVTGFDVDA
                     WKVGELNAGRSYIPDVPQPDLAKVVASGRFAATTAMDRLAEMDCIDIAVPTPLRKTKD
                     PDMSYIVQAVEAVKQHLRKGQLVSLESTTYPGTTDELVRPMLEETGLKAGRDFYLAFS
                     PERVDPANPQYLTKNIPKIVGGFDQASTDIACALYGSVVTKTVPVSSTVVAEMAKLLE
                     NTFRSVNIGMVNELALMCHRMKIDVWEVIDAAKTKPFGFMPFYPGPGLGGHCIPIDPF
                     YLSWKARQSGFEARFIELAGQVNGSMPEWIVTRIADALNTVKKAVNGSRIHVFGVAYK
                     KNVGDMRESPALDVIEHLSRRGASVTYTDPHVPSFRTDGRVFTAIGASEALAAAPDCV
                     VITTDHDAFDYKELVEKAALIMDTRNALKGFSGAHIHRL"
BASE COUNT          872 a         2147 c         2211 g          878 t
ORIGIN      
        1 gggccacgag atcggcgaag gcggcgctca ccgggtgccg cccccgcgca cgcgccggct
       61 cttcaggatc cgctcggcga actgctcttc ggtgacctgg atcggctcgt acgtcctgac
      121 gggcgcgccg atctgcaccg ggtagtcctt gcgcagcgga tgcccctccc actcgtcggg
      181 cgtgagcagc ctccgcaggt cgggatggcc gtcgaagacg acgcccagca ggtcccacac
      241 ctcgcgctcg aggaacccgg ccgacggcca gatccccgtg accgtcccga catgcggctc
      301 gggaccggcg agcctcacct tgacgcgcag ccgggccggc gcgtcggcgg ccaggaggct
      361 gtagctcaac tcgaagcggg gctcggcgcc ctggcgatcc acgccgacaa tgtcgaggag
      421 cgcggtgtag ccgagcgccg gatcgtcgcg gagcgccagg cacacctcgg cgaggcggtc
      481 ggccgagacg acgagcgtgg gctgatcgat cgcggccagc gcctgcgcgc ccgacccggg
      541 cacgagccgc tccagcaccg ccgcgacggc cgctgcgtcc atgggcatca cggcctcgcc
      601 gccgggtagg cgaacgcccg ctgccggtcg atcttgcgct gcaaggcgac aatcgcgtag
      661 agaagcgcct cgggccgcgg cgggcagccg ggcacgaaga cgtcgaccgg cacgacctgg
      721 tcgcagccct ggacgatcgc gtagttgtcg aagacgccgc cgcacgaggc gcacgcgccc
      781 atcgagatca cccacttcgg ctcgggcatc tgatcgtaga tccggcgcac ggccggcgcc
      841 atcttcctgg aaagccgccc cgcgacgatc atcaggtcgg cctgccgagg cgaggcgcgg
      901 aagacctcgg ccccgaaccg ggcgatgtcg tagcgcgacg ccgccatcga catcatctcg
      961 atggcgcagc aggccaggcc gagggtgacc ggccagatcg aggaccggcg cgcccactgc
     1021 accatcttgt cgacggtggt cgtcaggatg ggaatgtccc actcaggtgc catcggtcat
     1081 ccgtcgctgc ggcgccggcc cgggggcgcg gcgctgccgg cctacgggcg gttccagtcg
     1141 atcgcgccct tcttccagac gtacacgtac cccgccgcca ggaccaggaa gaacaccagc
     1201 acctggatga acccgaccca ccgcaggtcg cggatggcca tcgcccaggg gtagaggaac
     1261 gcaacctcga tgtcgaacag caggaagatc atcgccacca ggtagaactt caccgagtgg
     1321 cgctcgcggg catcgccgac cggcggcatg ccgcactcgt acggcgcgtc cttctccggg
     1381 gtccggtgac ggggcccgag agcccgcgat gccgcgatcg acacggcagc gaagccgagg
     1441 cccagcgcgg caagcaggaa gatcgacagc cagccctgca tacgcgggat tcatgctatc
     1501 ggacgagggg gaggcggggc aagacaaagt acccggacgc ccgcccgggt gcgggtctgg
     1561 cggcggcggg tgtggtaccg tggaggcgcc atgcgcatcg cctggttcag cccgctcccg
     1621 ccggaccgat cgggcatcgc cgcctacagc gccgacgtgc tggccggatt gcgcggccac
     1681 gagatcgacg cgttcatcga cgacacctcc ggccaggacg cggtcctgcg cgcgcggccg
     1741 ctggcgggcg cgagcatccg cggggcgcac gagttcgtct ggcggcgcgc gcgccgtccg
     1801 tacgacgtcg tggtgtacca gctgggcaac agcgcggcgc acgactacct ctggccgtat
     1861 ctcgtccgtt acccagggct cgtcgtcctt cacgacgcgc agctgcacca gtcgcgggcg
     1921 cgggcgctca tccgccagtt cagggaggac gactacaagg cggagtttcg ctacagctat
     1981 cccggcgtgc cgccgctcct tgccgacctc gtggtggcgg ggctcggcgc cacgctgtgc
     2041 tacttctggc cgatggttca gatccccgtc gagtcggccc gcaccgtcgc ggtccacaac
     2101 gcgcacctcg cctcggccct gcgcggccgc ttccccggct gcgacatcgt ccggatccac
     2161 atgggcgtgc ccgatcccgg cgttcccccc ggcgccgcgg cgggcgtgcg ccgccggcac
     2221 ggcattccgg aggacgcggt ggtgttcggg tccttcgggc gcgtcacgcc cgagaagggg
     2281 ctgacggcgg tcgtgcgggc cctcgcgcac gtggcgccga gcgttccgaa cctgcggctg
     2341 ctcaccgtcg gcgagccggt cgggtactac aacctcgtgg cccaggcgcg agaactgggc
     2401 gtcgccgacg tcgtcgtctc ggcgggctac gtcggggacg gcgaactcgc gtcctacctg
     2461 gcggcgacgg acgtctgcct gaacctgcgc tggcccacgg ggcgcgagac gtcggcggcg
     2521 tggctgcggt gcgtcgcggc cggcaagccc acgatcgtga ccgacctcgc gcaccagacc
     2581 gacgtgccga gcctcgacgt gcggaccatg gcagcggcgc cgggcggccc cgcgggcgcc
     2641 gagcccgtgt gcctgcacat cgagctggtg gacgagatcc cgatgctgcg cctggccctg
     2701 cagcgcctgt gcggcgacgc gggcctgcgg gagcggctgg gccgggcggc ccgccgctac
     2761 tgggaggagc acgccacggt cggcctgatg gtgcgcgact acgagggcct gctggcgcgc
     2821 gcggccgcgg cgcccgatcc gccgcggccg ccggcctggc ccgcgcacct ggcggccgac
     2881 gggtcggcgc tggcgagggc gctggcggcg gaggcgggcg tgccgtaccc gctcgcggcg
     2941 gggcccggcg gccccgaatc gtcggggcgc gcgtgaacga gggggcgtga tccatgtttg
     3001 ccgaggacga catcgagcgc cggagggtgg agcgtgaggc cgccgatcgc gcctacaacg
     3061 aggcgctcac ggcgctggac caggcgtttc cgcggatgcc cgagctgccg cacccgcccg
     3121 tcgtgtacga cgagagccgg ctgccggcga tcaacgagcg ctgggagatc gtcccggccg
     3181 atcccgcccc gggcgcgagc ggctggcgcc gccgcgcggc gcggctggtg tggagcatcg
     3241 tgcgcccggc gttcgagcgc cagcaggcat tcaactccgc gctcgtggac cacctgaacc
     3301 gcaacgtcgc cgtccaccgg gccaaccgcg aggcgattgc ggcggcgatc gagacgctgc
     3361 gcggggagct ggagaagctg caggcgttcc acagccggct ggtgatgtac ctgcagacga
     3421 tcacgcggtt cgtggacacg aaggaccgga gcgagcacat cgcgaggctc gccaacatca
     3481 cgcaggcggg cctgagcggc gtcgtggacg agttccgccg ggactgggag gcgatgacgg
     3541 cgcgcgacca gcgccatgtc gcggcggtgg acgagctgcg gaccagcatc gcggccgtgc
     3601 agcagaccgg cctcacgctc aagcgcgagc tcgagcggct cctcgcaggg cgggccgcgc
     3661 cggcggaggc gggtgcggcc gcggcgggac cggcgcagcc gggagccgag ccgaccggcg
     3721 gggcgcagca gacgaaggag gcccggccgg cgccgggcgc gggagcggtc ccgccggccg
     3781 ccgacgccgt gaacgcctat aagtacgtcg gcttcgaaaa caagttccgc gggtccgagc
     3841 cggagatccg cgcgcgcctc gaggactacg tcgggctctt tgcgggcgcc tcggacgtgc
     3901 tggacctcgg ctgcggccgt ggcgagttcc tcgcgctcct cgcggagcgg ggcatcgcgg
     3961 gccgcggcct cgacatcaac cacgagatgg tggaattgtg ccgcgcccgg gggctcgagg
     4021 cccgcgaagg cgacgctctc gcctacctcg agtcgctcgc ggacggggcc ctcggggggc
     4081 tgatcgcggt ccaggtcgtc gagcacttcc agccggccta cctcgtccgc ctgctcgacg
     4141 cggcgttcca caagctcagg ccggggtccc ggatcgtcct cgagacgctg aaccccgcgt
     4201 gctggttcgc gttcttcgac gcctacatcc gcgacatcac ccacgcctgg ccgctgcacc
     4261 cggagacgct gaagtacctg gtcgtcgcct cggggttcca gcgcgcggac gtgcggatgc
     4321 tgtcgccgta cccggccgac gcgcggcttc agccgttgtg gctgccgccc ggggccgaca
     4381 cgctcgccat gacgacgatg gcgcaggtgt tcaacgagaa catggccaag ctcaacgccg
     4441 tgatgttctc ggagctggac tacgcggtca ccgcggagcg gatgtaagga ctcggggctc
     4501 tgggctcggg actcggagct cgggactcgc gattcggggc tcggggctcg aggcttgcgg
     4561 ttcggggctt gtgaaggccg aagggccggc tgggtgcatc acccgccggc cctccgcgtg
     4621 tgtgtcttct gaatgccgga ttccgaattc cgatttccgg attctgactc cttctcagag
     4681 cctgtggatg tgcgcgccgc tgaagccctt cagcgcgttg cgcgtgtcca tgatcagcgc
     4741 ggccttctcg acgagctcct tgtagtcgaa agcgtcgtgg tcggtcgtga tgaccacgca
     4801 gtcgggcgcc gccgccagcg cctccgacgc gccgatggcg gtgaacacgc gcccgtcggt
     4861 gcggaacgac ggcacgtggg ggtcggtgta ggtcacactg gcgccgcgcc gggacaggtg
     4921 ctcgatgacg tccagggccg gcgactcgcg catgtcgccg acgttcttct tgtaggcgac
     4981 gccaaagacg tggatgcgcg acccgttgac ggccttcttc accgtgttca gcgcgtcggc
     5041 gatccgcgtc acgatccact cgggcatcga gccgttcacc tgcccggcca gctcgatgaa
     5101 gcgcgcctcg aagccgctct gccgcgcctt ccaggacagg tagaagggat cgatggggat
     5161 gcagtgcccg ccgagcccgg gccccgggta gaacggcatg aagccgaagg gtttggtctt
     5221 ggcggcgtcg atgacctccc agacgtcgat cttcatccgg tggcacatga gtgccagctc
     5281 gttcaccatc ccgatgttca ccgaccggaa ggtgttctcg aggagcttcg ccatctcggc
     5341 gacgacggtg gagctcacgg gcacggtctt cgtcacgacc gacccgtaca gggcgcaggc
     5401 gatgtccgtg ctggcctggt cgaacccgcc gacgatcttc gggatgttct tcgtcaggta
     5461 ctgggggttc gccgggtcca cgcgctcggg cgaaaacgcg aggtagaagt cccggccggc
     5521 cttcaggccg gtctcctcga gcatgggcct gaccagctcg tcggtcgtgc ccgggtaggt
     5581 cgtcgactcg aggctgacga gctgcccctt gcgcaggtgc tgcttcacgg cctcgacggc
     5641 ctggacgatg tacgacatgt ccgggtcctt cgtcttccgg aggggcgtcg gcacggcgat
     5701 gtcgatgcag tccatctcgg cgagccggtc catggccgtc gtggccgcga agcggcccga
     5761 ggccacgacc ttggccaggt ccggctgggg cacgtcgggg atgtagctcc ggccggcgtt
     5821 cagctccccc accttccagg cgtccacgtc gaagccggtc acgcggaacc cgccgagggc
     5881 gagttcgacg gcgaggggca ggccgacgta gcccagtcca atgacgccga cgttgcagtc
     5941 cttcctctga acgcgttcga gcagggtcat cgcagtcctc tagcgcggtt tcccgggtgt
     6001 gattcccgac aacttctcga tgtcgctgtc gaccatcatg ccgaccagcc cctcgaaatc
     6061 gaccgacggc gcccacccga gcacctgtcg ggccttcgcg gcgttgcc
//
LOCUS       DAOSIF010000135         6051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11799_length_6051_cov_4.604403, whole genome shotgun sequence.
ACCESSION   DAOSIF010000135 DAOSIF010000000
VERSION     DAOSIF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11799_length_6051_cov_4.604403"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLT35_05995"
     CDS             <1..520
                     /locus_tag="PLT35_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28816.1"
                     /translation="LDRPTSGWVEIDGRRLDGLRDDELTRVRRDTIGFIFQFFNLLPT
                     LSCLENVALPLHLRGWARKKAADRARELLGLVRLAARVDHLPDELSGGERQRVAIARA
                     LAIYPPILLADEPTGNLDSSTGDEILALVGDLHQRLQATVVIVTHDRHVAEICPRTIT
                     LRDGRIVGDERR"
     gene            517..3069
                     /locus_tag="PLT35_06000"
     CDS             517..3069
                     /locus_tag="PLT35_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG28817.1"
                     /translation="MMLLRLITWPYVRRHRLRTLLTTAGIVLGVAVFVGMRTANESVL
                     LAFRQTIDRIAGKAELQVAAGEGGLPEDVLERVQAMPEVRAAVPVIEAAVGTNLASEG
                     NVLVLAVDMTGDRSLRDYDLESGDEAVIDDPLVFLAQPDSLIVTREFAARNGLAIGST
                     VPMRTMEGEKRFTVRGIMKSGGLTQAFGGNLAVMDVYAAQMVFGRGRTFDRVDLALSE
                     GVGLERGRLAIGARLGPGYQVEPPASRGRQIDSVLRVYSFTADMNSAFALFIGMFIIY
                     NAFSIAVTERRREIGILRALGASRARIRALFLAESGIAGLAGSLAGLALGVLLSKGLA
                     LYISGLIAGVYGIAERTTEIATEPWLMAAGLLMGVATSLAAGWIPARNAARVDPIQAL
                     QKGRYQTLSAGENRVRRAAALALGGASLALLLFAKGWTVLFYAGYICAALALLLVSPT
                     VSLWLTKALRPPLRRLRPVEGALAADSLIQAPRRTSATVTALMLSLSLVIAMGGVARA
                     VYDSIMGWTRTTLNPDLFVTATQNLTERTFRFPESLGDQLEEVEGVEEIQRVRTHRIV
                     YEGSPIMLVAVEAKSMGDRVRRPPVEGPGDMYERAARGEGVILSDNFARLRGLHAGGS
                     IELDTPSGPRRVAVLGLVVDWTDQTGVVLIDRASYVRWWRDDTVNVFRVYVKNGARPQ
                     EVRQRIVERFAGAHRLFVLTNREVAAWIAGLTDQWMSLAYAQIAIAVFVAILGIVNTL
                     TVSIIDRRRELGVLQAVGGTRGQVRRTVWMEALAIGTVGLALGLGVGAVTLNYVLEMS
                     QRDVTGLALPYTFPWGTAALLVPLILASAFVAALWPAESAVRGSLVEALEYE"
     gene            3253..4029
                     /locus_tag="PLT35_06005"
     CDS             3253..4029
                     /locus_tag="PLT35_06005"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOG28818.1"
                     /translation="MTRCIRVLSVTLPLALGLVQPRTAAAQSAREVMEESQKRGQAAS
                     QHYEGLLQVVDARGKISDKRWIFDRLGSHGRSRAVLTFMEPPEVRGVALLVVNHPDRA
                     SDQWMWTPAIQRERRIALQDRSTRFFGTDFSFEDLEERDTEQYDFRAIADETIDGAAC
                     WKIEAKPRAGKSSQYTLSYVWVRKDNYAFQLVENYAKGALARRLRYSRIENVQGIWAG
                     RVLEMTDVRRNSRTVLTLDKLQYNLPMKEERFTLQALRRQ"
     gene            4071..5360
                     /locus_tag="PLT35_06010"
     CDS             4071..5360
                     /locus_tag="PLT35_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28819.1"
                     /translation="MHRRRVAFVLIAAALLPAAAAGQTVSHRGFLEVRGVGYPQTTAS
                     DSTRLVGEALVRHEVTAKPAPWLRLEGHADLRGDTHGQTDWGGPALSDRSTKRPALSI
                     RRLDAMLAKGPVSLDLGKQFVRWGKTDILNPTDRFAPRDYLAVVDSEILAVTGGRLTV
                     GLQSNALDLVAARFTPSRMPLVENRWSGITGGPAGTAFADGGAAHPARAQLGARWNHV
                     GSGFECSLSAFRGNTHLPVFAAGTPALPGGTAFPPAGPPPAGALVGPAPIRVARAFPE
                     MWMLGADAAVPLSPATIKGEAAFFGSGDARAGEYWLYVIQLERQRGEWVFVGGYAGEA
                     VTRPRAGGAFAPDRGLAKAFLGRASYTIDAARSAAFEGAVRQTGDGAWLRFEYSQASG
                     RHLRLTIRATIIGGAAGDFLGRYARNSHVTVGVRYSY"
     gene            5686..>6051
                     /locus_tag="PLT35_06015"
     CDS             5686..>6051
                     /locus_tag="PLT35_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28820.1"
                     /translation="MPVDLPADIADALTRAGLRAASAEVLTLTPDPAMPRALYRVRLD
                     CGRAVKARRFESEGEAARQQALRAVLPSAFAAVVARSGAVLIEEWIDGRSLADGAPDE
                     ATVRGAARLLAAMHATPELD"
BASE COUNT          818 a         2134 c         2233 g          866 t
ORIGIN      
        1 tctcgaccgg ccgacctcgg gctgggtcga gatcgacggc cggcgcctcg acggcctccg
       61 cgacgacgag ctgacgcgag tgaggcgcga cacgatcggc ttcatcttcc agttcttcaa
      121 cctgctgccc acgctcagct gcctcgagaa cgtcgccctg ccgcttcacc tgcgcggctg
      181 ggcccggaag aaggctgcgg atcgcgcccg ggagctgctc ggcctcgtgc gcctcgccgc
      241 gcgcgtcgat cacctgcccg acgagctgtc cggcggcgag cgccagcgcg tcgccatcgc
      301 ccgcgccctc gcgatctacc cgccgatcct cctggccgac gagccgacgg ggaacctcga
      361 ttcctcgacc ggcgacgaga tcctggccct cgtcggcgac ctccaccagc gcctccaggc
      421 caccgtcgtc atcgtcaccc acgaccgcca cgtggcggag atctgcccgc ggaccatcac
      481 cctccgcgac ggccggatcg tcggggacga gcggcgatga tgctgctgcg cctcatcacg
      541 tggccctacg tgcgccggca ccggctgcgg acgctgctca cgacggccgg catcgtgctg
      601 ggcgtggccg tgttcgtcgg catgcggacg gccaacgagt cggtgctcct cgcgttccgc
      661 cagacgatcg accgcatcgc gggcaaagcg gagctgcagg ttgccgccgg cgaaggcggg
      721 cttcccgagg acgtcctcga gcgcgtgcag gcgatgccgg aggtgcgcgc cgcggtcccg
      781 gtcatcgagg cggcggtggg cacgaacctc gcgagcgagg gcaacgttct cgtcctcgcg
      841 gtggacatga cgggcgaccg gtcgctgcgc gactacgacc tcgagagcgg cgacgaggcc
      901 gtcatcgacg acccgctcgt cttcctcgcg cagccggact cgctgatcgt gacgcgcgag
      961 ttcgccgccc gcaacggcct ggccatcggc tcgacggtcc cgatgcggac gatggagggc
     1021 gagaagcggt tcaccgtgcg cgggatcatg aagtcgggcg gcttgacgca ggccttcggc
     1081 ggcaacctcg ccgtcatgga cgtctacgcc gcccagatgg tgttcggccg cgggcgcacc
     1141 ttcgaccgcg tggacctcgc gctcagcgaa ggcgtggggc tggagcgggg gcggctggcg
     1201 atcggcgcgc gcctggggcc gggctaccag gtcgagccgc cggcctcgcg cggccgccag
     1261 atcgactcgg tgctccgcgt ctactccttc acggcggaca tgaacagcgc cttcgcgctg
     1321 ttcatcggga tgttcatcat ctacaacgcg ttctcgatcg ccgtgaccga gcggcggcgg
     1381 gagatcggca tcctgcgcgc gctcggggcc tcgcgcgccc ggatccgcgc gctgttcctc
     1441 gcggagagcg ggattgcggg gctcgccgga tcgctggccg gcctcgccct cggcgtcctg
     1501 ctgtcgaagg gcctggcgct ctacatctcc ggcctcatcg cgggcgtcta cggcatcgcc
     1561 gagcggacga cggagatcgc caccgagccc tggctgatgg cggcgggcct gctgatgggc
     1621 gtggccacga gcctggcggc gggctggatc ccggcgcgca acgccgcgcg cgtggatccg
     1681 atccaggcgc tgcagaaggg ccggtaccag acgctgtcgg ccggcgagaa ccgcgtccgg
     1741 cgggcggcgg ccctcgcgct cggcggcgcg tctctcgcgc tgctgctctt cgcgaagggg
     1801 tggaccgtcc tcttctacgc cgggtacatc tgcgccgcgc tcgcgctcct gctggtcagc
     1861 cccacggtgt cgctgtggct gacgaaggcg ctgcggccgc cgctgcggcg gctccggccc
     1921 gtcgagggcg cgctcgcggc cgacagcctc atccaggcgc cgcggcggac gtcggcgacg
     1981 gtgacggccc tcatgctgtc gctctcgctc gtcatcgcca tgggcggcgt cgcgcgggcg
     2041 gtctacgact cgatcatggg ctggacgagg acgacgctga acccggactt gttcgtcacg
     2101 gcgacgcaga acctcacgga gcgcacgttc cgcttccccg agtcgctcgg ggaccagctc
     2161 gaggaggtcg agggcgtcga ggagatccag cgcgtccgga cccaccggat cgtgtacgaa
     2221 ggctcgccca tcatgctcgt cgcggtggag gcgaagtcga tgggggatcg cgtgcggcgg
     2281 ccgccggtcg aaggccccgg cgacatgtac gagcgggccg cgcgcggcga gggcgtcatc
     2341 ctgtcggaca acttcgcgcg gctgcgcggc ctgcacgccg gcggctcgat cgagctggac
     2401 acccccagcg ggccgcggcg cgtggcggtg ctcggcctcg tcgtggactg gacggaccag
     2461 accggcgtgg tcctgatcga ccgcgccagc tacgtccgct ggtggcgcga cgacacggtc
     2521 aacgtcttcc gcgtctacgt gaagaacggg gcgcggccgc aggaggtccg gcagcggatc
     2581 gtcgagcgct tcgccggcgc gcaccggctg ttcgtcctca ccaaccgcga ggtcgccgcg
     2641 tggatcgcgg ggctcaccga ccagtggatg agcctggcct acgcgcagat cgccatcgcc
     2701 gtcttcgtgg cgatcctcgg catcgtcaac accctgaccg tgtcgatcat cgaccggcgg
     2761 cgcgagctgg gcgtgctgca ggccgtcggg ggcacgcggg gccaggtgcg ccggacggtg
     2821 tggatggagg cgctggcgat cgggacggtc gggctcgccc tgggcctggg cgtcggcgcg
     2881 gtgacgctga actacgtcct cgagatgagc cagcgcgacg tgaccgggct ggcgctgccg
     2941 tacacgttcc cctggggcac ggcggcgctg ctcgtgccgc tcatcctcgc atcggcgttc
     3001 gtcgcggcgc tgtggccggc ggagtcggcg gtcagaggat cgctggtgga ggcgctcgag
     3061 tacgaatagg gacaaggggc aagggggccg gggatcggtg actggaggtc gggaactgcg
     3121 gatcgggggc tgggcgccag ccggtctgga ctcgggactc ggcctgcctc gccgtagctg
     3181 gcccctcgac cagctcaggg tcggccggca gaccgcccag cgacggcggg ggccacgatt
     3241 cgggaacgaa cgatgacacg ctgcattcga gtcttgtcgg tcacgctgcc gctcgcgctg
     3301 ggcctggtgc agccacggac ggcggccgcg cagagcgcgc gcgaagtgat ggaggagtcg
     3361 cagaagcgcg ggcaggcggc gtcgcagcac tacgaggggc tcctccaggt cgtggacgcc
     3421 agggggaaga tcagcgacaa gcgctggatc ttcgaccggc tcgggtcgca cggccggagc
     3481 agggccgtgc tgaccttcat ggagccgccc gaggtcaggg gcgtggcgct gctcgtcgtg
     3541 aaccaccccg accgcgcgtc ggaccagtgg atgtggacgc cggcgatcca gcgcgagcgc
     3601 cggatcgcgc tccaggaccg atccacgagg ttcttcggca ccgacttcag cttcgaggac
     3661 ctcgaggagc gcgacacgga gcagtacgac ttcagggcga tcgccgacga gaccatcgac
     3721 ggcgcggcct gctggaagat cgaagcgaag ccgcgggccg gcaagtcgtc gcagtacacg
     3781 ctgtcctacg tgtgggtcag gaaggacaac tacgccttcc agctcgtcga gaactacgcc
     3841 aagggcgcgc tcgcgcgccg gctgcggtac tcgcgcatcg agaacgtcca gggcatctgg
     3901 gcgggccgcg tcctcgagat gaccgacgtg cgccgcaaca gccgaacggt cctgacgctt
     3961 gacaagctgc agtacaacct gccgatgaag gaagagcgct tcacgctgca ggccttgcga
     4021 agacagtagg aagaccccgg aagccggaat ccagaagaca gagcctcaag gtgcaccgtc
     4081 ggcgagttgc gttcgtcctc atagctgccg cattgctgcc ggcggcggcg gcgggccaga
     4141 ctgtctccca ccgcggcttc ctggaggtgc gcggcgtcgg gtacccgcag acgactgcgt
     4201 ccgacagcac gaggctcgtc ggcgaggcgc tcgtcaggca cgaggtgacg gcgaagcccg
     4261 ctccgtggct gaggctcgag gggcatgccg atctgagggg cgacacgcac ggccagacgg
     4321 actggggcgg cccggccctc tccgatcgat cgacgaagcg gccggcgctc tccatccggc
     4381 gcctcgacgc gatgctcgca aagggcccgg tctcgctcga cctgggcaag cagttcgtcc
     4441 gctggggcaa gaccgacatc ctgaacccga cggatcggtt cgcgccgcgc gactacctgg
     4501 ccgtcgtgga cagcgagatc ctcgcggtga caggcggccg gctgaccgtg gggctccagt
     4561 cgaatgcgct cgacctggtc gcggcgcggt tcacgccgag ccggatgccg ctcgtcgaga
     4621 atcggtggtc cggcatcacg ggcggcccgg cgggaacggc tttcgccgac ggcggcgccg
     4681 cgcatccggc ccgggcgcag ctcggcgcgc ggtggaacca cgtcgggagc ggcttcgagt
     4741 gctcgctgtc cgcgttcaga ggcaacaccc acctcccggt cttcgcagcc gggactcccg
     4801 ccctcccggg cggcacggcc tttccgcccg caggcccgcc tccggctggc gccctcgtcg
     4861 gccccgcgcc gatccgggtc gcacgcgcgt ttcccgagat gtggatgctc ggcgccgacg
     4921 ccgcggtccc gctctcgccg gccacgatca agggcgaagc cgccttcttc ggatcgggcg
     4981 acgcgcgcgc cggcgagtac tggctctacg tcatccagct cgagcgccag agaggcgaat
     5041 gggtcttcgt cggcggctac gcgggggagg cggtgacgag gccgcgcgcg ggcggagcgt
     5101 tcgcgccgga ccgcggcctg gcgaaggcgt ttctcggccg cgcgtcgtac acgatcgacg
     5161 ccgcccgcag cgccgccttc gagggcgcgg tccgccagac gggcgacggc gcgtggctcc
     5221 gattcgagta ctcgcaggcg tcgggacggc acctgcggct caccatccgc gccacgatca
     5281 tcggcggcgc ggccggagac ttcctcgggc gctacgcgcg gaactcccac gtcacggtgg
     5341 gggtgaggta cagctactga ctcaggaacc ggggcgcgaa tgcccgccgc cgccgggcgg
     5401 cctgtcggcc gccgccggcg aggcaggctg gattcggccc tccgatcctc cggccctccg
     5461 tctcgccggt ctcgggtccg ctcgccgtga gccgtccctc tcgcgggcgc ccggcgaacg
     5521 gcctcacgca ggctggcgtc cggcgggcgt ccggttcgaa gggcatccgg tcttgccacc
     5581 accatgcccg acgcggcaac atatgtcact ctctgccgcg agagaaccgg atggcgccac
     5641 gggtccgtgt gtcgtgtctg gtgccgtcag accctgcgag gccagatgcc tgtcgatctt
     5701 cccgccgaca tcgccgacgc gctgacccgc gccgggctcc gcgcggcgtc ggcagaagtc
     5761 ctgacgctca cgccggatcc cgccatgccg cgcgcgctgt accgcgtgcg gctggactgc
     5821 ggccgggcgg tcaaggcgcg gcggttcgag agcgagggcg aggcggctcg tcagcaggcc
     5881 ctccgcgcgg tgttgccgtc cgcctttgcc gccgtcgtcg cccggtcggg ggcggtgctg
     5941 atcgaggagt ggatcgacgg gcgctcgctg gccgacggcg cgcctgacga ggctacggtg
     6001 cggggcgccg cgcgcctgct ggccgccatg cacgcaacgc cggagctgga c
//
LOCUS       DAOSIF010000136         6043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11822_length_6043_cov_3.526052, whole genome shotgun sequence.
ACCESSION   DAOSIF010000136 DAOSIF010000000
VERSION     DAOSIF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11822_length_6043_cov_3.526052"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="PLT35_06020"
     CDS             <1..1659
                     /locus_tag="PLT35_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HOG28821.1"
                     /translation="VQAERQQDWDRAVVEYTAAVRAHPADQDARLALERVKLRATRQH
                     VTNGRRLASMGKVEEALVEYQIASQLSPASTEIEDALRSLRAAIRAKVSVAHDGKTEL
                     QTLVERTRDLPPPGLDLPSDARIPDSLTFREAGSRDVFTAIARFAGVSVVFDPAFRDA
                     PLTIDLRKATFEQALQSVAASTRSFYRLTAPRTLTIVPDTPAKRREYEEEVVRTFYLS
                     NADLKETIDLLRIVVDARKISSVAGTNAIALKDTPERVAAAAKLIAAIDKARPEVVID
                     VEVMEVDRVRLQEYGIQVATTGSTGVEGGAAVARDSLTLRDLRTLTTGDIALSSFPSL
                     FYRLLRADSSTRTLANPQLRALEGTAAQARFGERVPVPNTTFSPIATGGVNTQPITSY
                     TYENIGVNIDITPRTHHNDEVSLALKVEVSSISGTGYADLPTFGSRSVNSVIRLRDGE
                     TSILAGLIRDDERRTLEAVPGLSDLPLIGRLFARNRSERTETDIVITLTPHIVRVLDI
                     TENDLRPFRVGRDTGSPILDLLEPIRDEPAEPTAKPGTPPVIKD"
     gene            complement(1739..3145)
                     /gene="mpl"
                     /locus_tag="PLT35_06025"
     CDS             complement(1739..3145)
                     /gene="mpl"
                     /locus_tag="PLT35_06025"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HOG28822.1"
                     /translation="MPRVHLIGICGTAMAALAAMLKRRGCAVRGSDADVYPPMSTFLE
                     REGIPVLQGYDAAHITADLDLVVIGNAVSRGNAEVEEVLDRKIRYCSLPEAVREHFLW
                     GARSIVIAGTHGKTTTTALAGWLLVHGGLDPSVLVGGIAADLGGEGASYRLGLGREFV
                     IEGDEYDSAFFDKTAKFLKYLPDVAVVGNIEFDHADIYEDLASVRLAFRRLVNLVPRS
                     GLLVLGADSPDAAALRPVARSRVETFGLADGADWQAYDLEALPSGVRFRVQRARSPLG
                     LFEIPLAGSHNVRNALAAMAVAAEAGVDAGAMREGLATFRGVKRRLERVGTARGVTIL
                     DDFAHHPTAVRETLNAVRASHPDARIWAVFEPRSASSCRRIFQDAFAGSFGAADEVVV
                     ARVYRSDLPGPERLSAERLVEDLNASGTKARFLPDVDAIVGAIASEARDGDFVVIMSN
                     GGFGGIHGRLLAALQRDS"
     gene            complement(3153..4133)
                     /locus_tag="PLT35_06030"
     CDS             complement(3153..4133)
                     /locus_tag="PLT35_06030"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOG28823.1"
                     /translation="MRDSRLVKPMALRPGDRVAVVAPSSAFPREDFFAGLDELRRLGF
                     EPVFDERVFGHHRYTAGEPVARARAFAEAWQDPGVRAVLAARGGYGSAGLLPWLSLEA
                     LARTPKILVGFSDVTALLSVLTTRCGMTAVHGPVVAGGLAAGPGRYDRHTFLQSLTSG
                     EPLGALAAPALEALAEGDAEGPIVGGNLTQVAASMGTPYAFDPPEGCVLFLEDVNERP
                     YRLDRLLTQLSHAGILHRARAVVFGEMPGCDEPDGSVAAKDAIGEVLRGFSGPVLLGL
                     PAGHTAGPALTLPLGVRARVATRPSPVVEILESAVRDGPGPRVPGPDSRK"
     gene            complement(4126..5214)
                     /gene="nagZ"
                     /locus_tag="PLT35_06035"
     CDS             complement(4126..5214)
                     /gene="nagZ"
                     /locus_tag="PLT35_06035"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOG28824.1"
                     /translation="MSPLGPRRRVGQLVFLGFDGFEIPPEIRSLAREFDLGGVALFKR
                     NVEGPGQVADLAFQAAALGLSAPAWVAVDQEGGRVARMRSPFTEWPPMAALGRSGNAG
                     LAARIGRAIAHELRAVGVTMDFAPVLDVLTNAANPAIGDRALSEDAGVVARLGAAMIR
                     AMQDEGIAACGKHFPGHGDAGVDSHHDLPVVGLSPDRFEAVEWVPFRAAMAESVAALM
                     VAHLLVPAFDEAVPASLSRRVVTGLLRERLRFDGLILTDDLSMKGCSLTCAPPAAAVR
                     AIAAGHDAALLCEPDHDGQAAALEALVRALEDETIAASQVEASLARHARAKARFAEPR
                     TGRPASAWREVVGCEAHQALAAELRRYA"
     gene            5264..5518
                     /locus_tag="PLT35_06040"
     CDS             5264..5518
                     /locus_tag="PLT35_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28825.1"
                     /translation="MDPVAEIKRLYYATTRATIQRDLDKAIALLKTLSTEEERERVGV
                     FMDGLSQMRSEWGVAPAAGKASARRKPSRRPAPRAVKGRG"
     gene            complement(5515..>6043)
                     /locus_tag="PLT35_06045"
     CDS             complement(5515..>6043)
                     /locus_tag="PLT35_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28826.1"
                     /translation="GAGASSDDPLVGADDGGAARGDVREDPDEAREAGRPTAAAGLRL
                     SSMTVPEKIKTAMRGTREERAILIRDPNKIVAFSVLSSPKLSEQDIEGFAGNAGLSED
                     VLRVIATTRAWCKNYSIVRALVFNPKTPIAISMSLVNRLVERDVRMLALDRNVAEPVR
                     LLARKSLQTGRSRRG"
BASE COUNT          939 a         2214 c         2107 g          783 t
ORIGIN      
        1 gtccaggcgg agcggcagca ggactgggac cgcgcagtgg tggagtacac ggcggccgtg
       61 cgcgcccatc ccgcggacca ggacgcccga ctcgccctcg agcgcgtcaa gctccgcgcg
      121 acgcgccagc acgtgacgaa cgggcggcgc ctggcgtcga tgggcaaggt cgaggaggcg
      181 ctggtcgagt accagattgc ctcgcagctg tcgccggcga gcacggagat cgaggacgcg
      241 ctccgcagcc tcagggcggc catccgcgcg aaggtcagcg tggcccacga cgggaagacc
      301 gagctccaga cgctcgtcga gcgcacccgg gatcttcctc cgccgggcct cgatctgccc
      361 tcggacgctc ggattcccga ctcgctcacg ttccgcgagg ccggctcgcg cgacgtcttc
      421 acggccatcg cccggttcgc cggcgtcagc gtggtcttcg atccggcgtt ccgcgacgcg
      481 ccgctgacga tcgatctccg caaggcgacc ttcgagcagg cgctccagtc cgtcgcggcc
      541 agcacccgct cgttctaccg tctgacggcg ccgcggacgc tcaccatcgt accggacacg
      601 cccgccaagc ggcgcgagta cgaggaggag gtcgtccgga cgttctacct gtcgaacgcg
      661 gatctgaagg agacgattga cctgctccgg atcgtcgtcg acgcgcggaa gatctcctcc
      721 gtcgccggca ccaatgccat cgcgctcaag gacacgcccg agcgcgtggc ggcggcggcc
      781 aagctcatcg cggcgatcga caaggccagg cccgaggtcg tcatcgacgt cgaggtgatg
      841 gaggtcgatc gcgtccgcct gcaggagtac ggcatccagg tcgcgacgac ggggtcgacg
      901 ggcgtcgaag gcggcgcggc cgtcgcccgc gactcgctga ccctcagaga cctgcgcacc
      961 ctgacgacgg gggacatcgc gctctccagc tttccgtcgc tgttctaccg gctgcttcgc
     1021 gccgactcga gcacgcggac gctggcgaac ccccagctcc gcgccctgga gggcacggcc
     1081 gcccaggccc gattcgggga gcgcgtgccg gtgcccaaca ccacgttctc ccccatcgcg
     1141 acgggcggcg tgaacacgca acccatcacg tcgtacacgt acgagaacat cggcgtgaac
     1201 atcgacatca cgccgcgcac ccaccacaac gacgaggtct cgctggcgct gaaagtggag
     1261 gtgagctcga tctcgggcac gggctacgcc gacctgccga cgttcggcag ccgatcggtg
     1321 aactcggtga tccggctgcg cgacggcgaa acgagcatcc tcgccggcct catccgcgac
     1381 gacgagcggc ggacgctgga agcggtgccg ggactcagcg acctcccgct catcggccgg
     1441 ctgttcgcgc gcaacaggag cgaacgcacc gagaccgaca tcgtcatcac gctgacgccg
     1501 cacatcgtgc gggtcctcga catcaccgag aacgacctgc ggcccttcag agtcggccgc
     1561 gacaccggca gcccgattct cgacctgctg gagccgatcc gggacgagcc ggccgaaccg
     1621 acggcgaagc cggggacgcc gccggtcatc aaggactagg ggcgaggcgc gagtcgggca
     1681 gggagcgggg gctggggatt ctggattctg aagtccggat tctgaatacc gccagcgtct
     1741 agctgtcgcg ctggagggcg gcgagcagcc ggccgtgaat cccgccgaac cctccgttgg
     1801 acatgatcac gacgaagtcg ccgtcgcggg cttccgacgc gattgccccg acgatggcat
     1861 cgacgtccgg gaggaagcgg gcctttgtcc cgctggcgtt gagatcctcc accaggcgtt
     1921 cggccgagag gcgctcaggc ccgggcagat ccgatcggta cacgcgcgcg accacgacct
     1981 cgtctgccgc gccgaacgac ccggcgaagg cgtcctggaa gatccgacgg cacgacgagg
     2041 cggaccgcgg ctcgaacacc gcccagatgc gcgcgtccgg gtgcgaggcg cggacggcgt
     2101 tcagcgtctc cctgacggcc gtcggatggt gcgcgaagtc gtcgaggatg gtcacgccgc
     2161 gcgccgtccc cacgcgctcg agccggcgct tcacgccccg gaacgtggcg aggccctcgc
     2221 gcatggcgcc ggcgtcgacg ccggcctcgg cggcgaccgc catggcggcc agcgcattcc
     2281 tgacgttgtg cgatcccgca agcgggatct cgaacagccc cagcggcgag cgcgcgcgct
     2341 gcacgcggaa ccgcactccc gaaggcaggg cctccaggtc gtacgcctgc cagtcggcgc
     2401 cgtcggccaa accgaacgtc tcgacgcgcg accgcgcgac tggccgaagc gccgcggcat
     2461 cagggctgtc ggcgccgagg acgagcagcc cgctgcgcgg cacgaggttc accaggcgcc
     2521 ggaacgcgag acggaccgac gccaggtcct cgtagatgtc ggcgtggtcg aactcgatgt
     2581 tgccgacgac ggcgacgtca ggcaggtact tcaggaactt cgcggtcttg tcgaagaacg
     2641 cgctgtcgta ctcgtcgccc tcgatgacga actcccggcc gaggcccagc cggtaactcg
     2701 cgccctcgcc acccagatca gccgcgatgc cgccgacgag cacgctcggg tcgagcccgc
     2761 cgtgcacgag cagccacccg gccagcgcgg tcgtggtcgt cttgccgtgc gtcccggcga
     2821 tgacgatcga ccgcgcgccc cagaggaagt gctcgcgcac cgcctccggg agcgagcagt
     2881 agcggatctt gcgatccagg acttcctcga cctcggcgtt gccgcgcgag acggcgttgc
     2941 cgatgaccac caggtccaga tcggcggtga tgtgcgcggc gtcgtacccc tggagcacgg
     3001 ggatgccctc gcgctcgagg aatgtgctca tcggcgggta gacgtccgca tcggacccgc
     3061 ggacggcgca ccccctgcgc ttcagcatcg ctgccagcgc ggccatggcg gtgccgcaga
     3121 tgccgatcag atggacgcgt ggcatggggg catcactttc gggagtcggg gccagggact
     3181 cggggcccgg ggccgtcgcg aacggccgat tccaggatct cgactacagg ggatggccgc
     3241 gtggcgacgc gggcgcgcac gccgagcggc agcgtgagcg ccgggccggc cgtgtggccc
     3301 gccgggagcc ccagaagcac cggaccgctg aagccgcgga gcacctcgcc gatggcgtcc
     3361 ttcgcggcga cgctcccgtc gggctcgtcg cagcccggca tctcgccgaa cacgacggcg
     3421 cgcgcgcgat gcaggatgcc ggcgtgagag agctgcgtga gcaggcgatc cagccggtac
     3481 ggccgctcgt tgacgtcctc gaggaacagc acacacccct ccggcgggtc gaaggcgtac
     3541 ggggtgccca tcgaagccgc cacctgggtc agattcccgc cgacaatcgg cccctccgcg
     3601 tcgccttccg ccagcgcctc cagcgccggc gccgccaggg cgcccaacgg ctcgccgctc
     3661 gtcagcgact gcaggaacgt gtgccggtcg tagcgcccgg gccccgccgc gaggccgcca
     3721 gccacgactg gcccgtgcac cgccgtcatg ccgcaccgcg tcgtcagcac gctcaagagc
     3781 gcggtcacgt cgctgaagcc cacgaggatc ttcggcgtgc gcgcgagggc ctcgagagag
     3841 agccacggga gcagcccggc gctgccgtac ccgccgcgtg cggccagcac cgcccggacg
     3901 cccgggtcct gccacgcttc tgcgaaggcc cgggcccgcg cgacaggttc ccccgccgtg
     3961 tagcggtgat ggccgaagac gcgctcgtcg aaaacgggct cgaagccgag gcgccgcagc
     4021 tcgtcgaggc ccgcgaagaa gtcctcgcgg gggaacgcgc tcgacggcgc cacgacggcg
     4081 actcggtcgc ccggccggag cgccatcggc ttcacgagcc ggctgtcacg catagcgccg
     4141 gagctccgcc gcgagcgcct gatgggcttc gcagcccacg acctcccgcc aggccgaggc
     4201 gggacgtccg gtccgcggct cggcgaacct ggctttcgcg cgggcgtgcc gggccagcga
     4261 cgcctcgacc tgtgaggccg cgatggtctc gtcctcgagg gcgcgcacga gggcctcgag
     4321 ggcggctgcc tggccgtcgt ggtccggctc gcacaacagc gccgcgtcgt gtccggccgc
     4381 aatcgcccgc acggctgcgg ccggcggcgc gcaggtcagc gagcagccct tcatgctgag
     4441 atcgtcggtg aggatcaagc cgtcgaaccg cagccgctcc cggagcaggc ccgtcaccac
     4501 ccggcgcgac agactcgccg ggactgcctc gtcgaatgcc ggcacgagca ggtgcgccac
     4561 catcagcgcc gcgaccgact cggccatcgc cgccctgaac gggacccatt cgacggcctc
     4621 gaaccggtcc ggcgacaggc ccaccaccgg caggtcgtga tgcgagtcca cgccggcgtc
     4681 tccgtggccc gggaagtgct ttccgcacgc ggcgatccct tcgtcctgca tcgcccggat
     4741 catggcggcg ccgagccggg ccacgacgcc ggcgtcctcc gacagcgcgc ggtcgccgat
     4801 ggccgggttg gcggcattcg tcagcacgtc cagcacgggc gcgaaatcca tcgtgacgcc
     4861 cacggcgcgg agttcgtgcg cgatggcccg gccgatgcgc gcagccagtc ccgcgttgcc
     4921 gctccggccg agcgccgcca tcggcggcca ctcggtgaac gggctgcgca tacgggccac
     4981 acgcccgcct tcctggtcca ccgcaaccca cgccggagcc gacaaaccaa gggcggccgc
     5041 ctggaacgcc aagtcggcca cctgtccggg gccctcgacg ttcctcttga agagcgcgac
     5101 gccccccagg tcgaattccc gcgccagcga tcggatctcc ggcgggatct cgaacccgtc
     5161 gaacccgagg aagacgagct gcccgacgcg gcggcgaggc cccagcggcg acatggaaac
     5221 ccattataat gacgccggac tccgccagcc gggggcattg agcatggacc cagtcgccga
     5281 gatcaagcgc ctctactacg cgacaacgcg cgcgacgatc cagcgggacc tcgacaaggc
     5341 catcgccctc ctgaaaaccc tgtccacgga ggaggagcgg gagcgcgtcg gggtcttcat
     5401 ggacgggctc tctcagatgc gctccgagtg gggggtcgcg cctgcggccg ggaaagcgtc
     5461 agcgcggcgc aagccgagcc ggcgtccggc cccccgcgcc gtgaagggca ggggctagcc
     5521 gcggcgcgac cgcccggttt ggagcgactt gcgggcgaga aggcggacgg gttccgccac
     5581 gttgcgatcc agcgcgagca tccggacgtc ccgctcgacg aggcggttca ccagcgacat
     5641 cgagatcgcg atgggcgtct tcggattgaa cacgagcgcc cggacgatgc tgtagttctt
     5701 gcaccaggcg cgcgtcgtgg cgatgacgcg cagcacgtcc tccgacaggc cggcattccc
     5761 ggcgaaccct tcgatgtcct gttcggacag cttcgggctg ctcagcacgg agaaggccac
     5821 gatcttgttc ggatcgcgga tgaggatcgc tcgctcctcg cgggtgccgc gcatggcggt
     5881 cttgatcttc tccggtaccg tcatggagga gagccgcagc ccggcggccg cggtgggccg
     5941 cccggcctcg cgcgcttcgt ccggatcttc ccggacgtcg ccgcgtgcgg cccccccgtc
     6001 atcggctccc acgagcgggt cgtccgacga ggccccagcg ccc
//
LOCUS       DAOSIF010000137         6042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11824_length_6042_cov_4.422582, whole genome shotgun sequence.
ACCESSION   DAOSIF010000137 DAOSIF010000000
VERSION     DAOSIF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11824_length_6042_cov_4.422582"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLT35_06050"
     CDS             complement(<1..364)
                     /locus_tag="PLT35_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG28827.1"
                     /translation="MLHSLAAFVFVLGVLIFVHELGHFVVARRLGVRVVTFCLGMGPK
                     LVKWRRGETEYAIAALPIGGYVKMAGENPDDPQTGARDEFLSRPKWDRLRILLAGPLM
                     NVLLAVALTTIVVAQGAQV"
     gene            complement(379..1539)
                     /locus_tag="PLT35_06055"
     CDS             complement(379..1539)
                     /locus_tag="PLT35_06055"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOG28828.1"
                     /translation="MRRIAILGSTGSIGRSALSVAEAHPDRLEVVSLAAGSNAALLAA
                     QVSRVRPRVAAMATPRALESLRAHLGAAAGARLVSGPDGLIEAATHPDADVLLCASSG
                     TAALEAVLAAIEAGKTIALANKEVLVMAGALVTSAARRRGVAILPVDSEHNAIHQCLH
                     GRDRAEIARLILTASGGPFRGHTLAQMKQVGPDDALRHPTWRMGRKITIDSATLMNKG
                     LEVIEARWLFGVGPAQIDVVVHPQSVVHSMVELRDGSIIAQLGVADMRLPIQYAFSYP
                     ERWESPLPRLDLARCGRLDFAVADHQRFPCLGLAYRALEGDSALPVVLNAANEVAVAA
                     FLGGTLGFLAIPDLIERTMEASPAGNVSTLADVRSVDRWARGVAQDLASGYN"
     gene            complement(1536..2321)
                     /locus_tag="PLT35_06060"
     CDS             complement(1536..2321)
                     /locus_tag="PLT35_06060"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG28829.1"
                     /translation="MTRFVSGLVMIAAFAGVLWYAPPWLFLVVIEILVVAAVLEYAGL
                     MERTGVPVPRPLAAAAAVVICAGIAWPGPYAVPGLAAAVVVVLLAALTASEPDPRVPA
                     AVAAALFPSLYIAVPLALAAVVRTDHGREALLLCVLVVLASDTAQYYTGTLVGRHRLA
                     PSVSPKKSVEGAAGGFVAGVAIAAGLGRLWLPAVPVPRLVAIGIVLVGLGIAGDLFES
                     LLKRSANVKDSSALIPGHGGVLDRIDAYLFVIPGYYLVLRLAA"
     gene            complement(2318..3055)
                     /locus_tag="PLT35_06065"
     CDS             complement(2318..3055)
                     /locus_tag="PLT35_06065"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOG28830.1"
                     /translation="MAHRLDPLKLPAHIAIIMDGNGRWAARRHLPRVQGHQAGVDAVR
                     DAVEACARMGISVLTLYAFSTENWKRPQLEVTTLMRLLKHYLKQERHTLVEHHIRFRM
                     IGRHEGLPADIRAELASAERETADAAGMQFNIALNYSGRAEIVDAARRAVALGLGADA
                     LDEQRFGSLLYTAGQPDPDLLIRTSGEMRVSNFLLWQIAYAEIHVTDVLWPDFRCRHL
                     LEAVLDYQQRERRYGGIKQPARRRRSP"
     gene            complement(3236..4036)
                     /gene="truA"
                     /locus_tag="PLT35_06070"
     CDS             complement(3236..4036)
                     /gene="truA"
                     /locus_tag="PLT35_06070"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG28831.1"
                     /translation="MKRVLRLTLAYEGTAYVGWQRQASGTSIQGLLEEALERLTGAPV
                     AVHGAGRTDAGVHAFGQVASARVDTGLEAAVIRRALNAVLPPDVRVLAIDDAGLEFHA
                     RYSASGKAYEYRIWEGTVQPPFARAWSWHHPRPLDVAAMDAGARLLEGRHDFTAFQSA
                     GSRVRSAVRTIVCARVGVDDPGPAVLGRAAARGGLAEAGRLLVLSVEADGFLRHMVRA
                     IAGTLVEVGEGRRDPGSVGALLQSRDRSAAGATAPPHGLVLVRVLYPA"
     gene            complement(4109..4699)
                     /gene="pyrE"
                     /locus_tag="PLT35_06075"
     CDS             complement(4109..4699)
                     /gene="pyrE"
                     /locus_tag="PLT35_06075"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOG28832.1"
                     /translation="MSPDDVLRRFTELGALLEGHFRLTSGLHSPTYLQCALVLQHPRE
                     AEALGRLLGAAVAGRLAGAAPTVVLSPAIGGLIIGHEVARALGVRAIFAERADGVLTL
                     RRGFDLAADDRVVIIEDVMTTGGSTRETIEVARAKGATVVAAGTVINRSGSPNPVDVP
                     FVALAAITPPTYAPEACPLCEQGIPVTKPGSRAEAK"
     gene            complement(4720..>6042)
                     /locus_tag="PLT35_06080"
     CDS             complement(4720..>6042)
                     /locus_tag="PLT35_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG28833.1"
                     /translation="ALDPETGKARVLDALRDEAWVREAGARGGGGLGWMPDGHSLWFL
                     SERDGWMHLYRVDAARPGSAPEQLTSGRFEVSSAQISPDKASFHLVTSEAHPGERHLY
                     AMPVAGGPRVRLTAVAGSCTGEASPDGAVFALIHSTATKPPEVYVMPNRAGAPMQQVT
                     LTPTEEWRAFRWIEPEVVTFKARDGAEVHARLFTPEMIGARRDRNRPGVVFVHGAGYA
                     QNAHRYWSSYYREFMFEHLLASRGYVVLDVDYRASAGYGRDWRTAIYRHMGGKDLEDV
                     VDGAKYLADAHRVDPRRIGVWGGSYGGFITLMAMFTAPGTFAAGAALRPVTDWAHYNH
                     AYTASILNQPQDDAEAYARSSPIRFAEGLKGALLICHGLVDTNVHAQDSIRLAQRLIE
                     LRKRNWELAVYPVENHSFEQASSWADEYSRILKLFETNLRLAPAAGGR"
BASE COUNT          892 a         2195 c         2175 g          780 t
ORIGIN      
        1 gcacctgcgc gccctgggcc accacgatgg tcgtcagcgc gacggccagg aggacgttca
       61 tcagcggccc ggccagcagg atgcggagcc ggtcccactt ggggcgcgag aggaactcgt
      121 cgcgggcgcc cgtctgcggg tcgtcggggt tctcgccggc catcttcacg tagccgccga
      181 tggggagcgc ggcgatggcg tactccgtct cgccccggcg ccacttgacc agtttgggcc
      241 ccatcccgag gcagaacgtg acgacgcgga cgccgagccg ccgggccacg acgaagtgcc
      301 cgagttcgtg cacgaagatg aggacaccca ggacgaagac gaaggcagcc agcgaatgca
      361 gcacgggacc tctcccgatc aattgtagcc ggaggcgaga tcctgcgcga cgccccgcgc
      421 ccagcggtcc acgctgcgga cgtcggccag cgtcgagacg ttcccggcgg ggctcgcctc
      481 catggtccgc tcgatgagat ccgggatggc gaggaagccc agcgtgcccc cgaggaacgc
      541 ggccacggcg acctcgttcg cggcgttcag gacgaccggg agcgccgagt cgccctcgag
      601 ggcgcggtag gccaggccga ggcaggggaa gcgctggtgg tccgccacag cgaagtcgag
      661 gcggccgcag cgcgcgaggt ccagccgcgg cagcgggctc tcccatcgtt cgggatacga
      721 gaaggcgtac tggatgggca agcgcatgtc ggcgacgccg agctgcgcga tgatggatcc
      781 gtcccgcagc tccaccatgg agtgcaccac cgactgcggg tggacgacga cgtcgatctg
      841 ggccgggccg acgccgaaga gccagcgcgc ctcgatgacc tcgaggccct tgttcatcag
      901 cgtcgccgag tcgatcgtga tcttccgccc catccgccac gtcgggtggc gcagggcgtc
      961 gtccggcccc acctgcttca tctgcgccag cgtgtggcct cggaacggcc cgccggacgc
     1021 cgtgaggatc aggcgcgcga tctcggcgcg gtcgcggccg tgcaggcact ggtggatcgc
     1081 gttgtgctcg ctgtccaccg gcaggatggc gacgcctcga cgccgcgccg ccgaggtcac
     1141 gagcgcgccc gccatcacga gcacttcctt gttggcgagg gcgatcgtct tgcccgcctc
     1201 gatggccgcg agcacggctt ccagggcggc cgtgcccgac gaggcgcaca ggagcacgtc
     1261 ggcgtcggga tgcgtggccg cctcgatgag gccgtccggg ccggacacca ggcgcgcgcc
     1321 ggcggcggcc ccgaggtgcg cgcggagcga ctccagggcg cgcggcgtcg ccatggctgc
     1381 cacgcgggga cggacccgcg acacctgcgc ggccagcagc gccgcgttcg agccggcagc
     1441 aaggctgaca acctccaggc gatccgggtg ggcctcggcc accgacagcg cgcttcggcc
     1501 aatcgagccc gtggagccga gaatcgcgat gcgcctcacg cggccagcct caagacgagg
     1561 tagtaacccg ggatgacgaa gaggtaggcg tcgatgcggt cgagcacgcc gccgtggcca
     1621 ggaatgagcg ccgagctgtc cttgacgttg gcgctgcgct tcagcagcga ctcgaagaga
     1681 tcgcccgcga tgccgaggcc gacgagcacg atgccgatgg ccacgaggcg cgggacgggc
     1741 accgcgggca gccagagccg gccgagcccg gcggcaatcg ccacgccggc gacgaagccg
     1801 cctgccgcgc cctccacgct cttcttcggg ctgacggagg gagccagccg gtgccggccg
     1861 acgagcgtgc ccgtgtagta ctgcgccgtg tccgacgcca ggacgaccag gacgcagagc
     1921 agcagcgcct cccggccatg atccgtccgc acgacggcag ccagggcgag cggcacggcg
     1981 atgtagagcg acgggaacag cgcggccgcc acggccgccg gaacgcgcgg atccggctcc
     2041 gacgccgtca gcgccgcgag caggactacc acgacggcgg ccgccaggcc gggcacggca
     2101 tacggccccg gccacgcaat ccccgcgcaa atcacgactg ccgcggcggc ggccagcgga
     2161 cgggggacgg gcacgccggt ccgctccatc aacccggcgt actccagcac cgccgcgacg
     2221 acgaggatct cgatgaccac gaggaagagc cacggcggcg cgtaccacag cacgcccgcg
     2281 aacgcggcaa tcatgaccag cccgctgacg aagcgcgtca cggggatctg cgcctgcggg
     2341 cgggctgctt gatgccgccg tagcggcgct cccgctgctg gtagtcgagc accgcctcga
     2401 gcaggtggcg gcaccggaag tcaggccaca gcacgtcggt gacgtggatc tcggcgtagg
     2461 cgatctgcca caagaggaag ttgctgacgc gcatctcccc gctcgtgcgg atgagcaggt
     2521 ccgggtccgg ctggcccgcg gtgtacagca ggctgccgaa ccgctgctcg tcgagggcgt
     2581 ccgcgccgag ccccagcgcg acggcgcggc gggcggcatc cacgatctcc gcccgcccgc
     2641 tgtagttgag ggcgatgttg aactgcatgc ctgcggcgtc agccgtctcg cgttcggcgc
     2701 tggccagctc ggcccggatg tcggccggaa ggccttcgtg gcggccgatc atccgaaagc
     2761 ggatgtggtg ctcgaccagc gtgtggcgct cctgcttcag gtagtgcttc aggaggcgca
     2821 tcagcgtcgt cacttcgagc tgcggccgct tccagttctc ggtcgagaag gcgtagagcg
     2881 tcaggacgct gatgcccatc cgcgcgcagg cttcgaccgc atcgcgcacg gcgtccacgc
     2941 cagcctgatg gccctgaacg cgcggcaggt ggcgccgggc ggcccaccgc ccgttgccgt
     3001 ccatgatgat ggcgatgtgc gcaggcagct tgagcggatc caggcggtgc gccaaggcct
     3061 cttctggcga ccccggtcgc agccacgcca gcagatggtc aagcgccatg aacgcaccca
     3121 gggaagactc gatccttcat gatacaggct tcgcaacatg ggcgccaccg cgcgccccgg
     3181 ggcgcgcggc cccgcaggat gccgggcggg cgccgggaac gcgggacgcg ggcgctcagg
     3241 cggggtacag gacgcgaacc agcacgagcc cgtgcggcgg ggctgtggcg ccggcggcag
     3301 agcggtcccg cgactggagg agcgcgccga ccgacccggg atcgcgccgc ccttcgccca
     3361 cctccaccag cgtgccggcg atggcgcgga ccatgtgcct caggaagccg tccgcctcca
     3421 cgctcaggac gagcaggcgg ccagcctcgg cgagtccgcc gcgcgccgcc gcgcgcccca
     3481 gaacggcagg cccgggatcg tccacgccca cgcgcgcgca gacgatggtg cgcacggccg
     3541 acctgacgcg gctgcccgcg gactggaacg ccgtgaagtc gtggcggcct tccagcagcc
     3601 gagcgcccgc gtccatcgcc gccacgtcga gaggccgggg atggtgccag ctccacgccc
     3661 tggcgaacgg gggctgcacg gtcccctccc agatccggta ctcgtaggcc ttgccgctcg
     3721 cgctgtacct cgcgtggaac tccaagccgg cgtcgtcgat ggcgagcacg cgcacgtcgg
     3781 gcggcagcac ggcgttgagg gcccggcgaa tcacggctgc ttcgaggccg gtgtccaccc
     3841 tcgcggaggc cacctggccg aaggcgtgca cgccggcatc cgtccgtccg gcgccgtgga
     3901 cggcgaccgg cgctcccgtc agccgctcca gcgcctcctc gagcaggccc tggatcgacg
     3961 tcccgctggc ctgccgctgc cagccgacgt aggccgttcc ctcgtaggcg agcgtgagtc
     4021 tcaggacccg cttcatcgat gctgcagggc cggccccggc gggccgctca ctccgaaggc
     4081 cggatgtcgg attccggacc ccgcctccct acttcgcctc cgcccgcgat cccggcttcg
     4141 tgaccgggat cccctgctcg cagagcgggc acgcctcggg cgcgtaggtc ggcggcgtga
     4201 tggccgccag cgccacgaac gggacgtcca ccgggttcgg cgagccgctg cggttgatta
     4261 ccgttcccgc ggccacgacc gttgcgccct tcgcccgcgc gacctcgatg gtctcgcggg
     4321 tggagccgcc cgtggtcatc acgtcctcga tgatcacgac gcgatcgtcc gcggcgaggt
     4381 cgaagccgcg gcggagcgtc aggacgccgt cggcgcgttc ggcgaagatc gcgcggacgc
     4441 cgagcgcgcg cgccacctcg tggccgatga tgaggccgcc gattgccgga gagagcacca
     4501 ccgtcggggc cgcgccagcc agacgcccgg cgacggccgc gccgagcaga cggccgagcg
     4561 cctccgcctc ccgagggtgc tggagcacca gcgcgcactg caggtaagtc gggctgtgga
     4621 gcccggaggt caggcggaaa tggccctcga gcagcgcgcc aagctcggtg aaccgccgga
     4681 ggacgtcgtc aggactcatc ggaggagact ccctggggat caacggccgc cggccgccgg
     4741 cgccaggcgc aggttggttt cgaacagctt caggatgcgc gagtactcgt cggcccagct
     4801 cgacgcctgc tcgaagctgt ggttctcgac cgggtagacg gcgagctccc agttccgctt
     4861 ccgcaattcg atcaggcgct gcgcgagccg gatggagtcc tgggcgtgca cgttggtgtc
     4921 cacgaggccg tggcagatga gcagcgcgcc cttcagcccc tcggcgaagc ggatcggcga
     4981 gcttcgcgcg tacgcctccg cgtcgtcctg cggctggttg aggatgctcg ccgtataggc
     5041 gtggttgtag tgcgcccagt cggtcaccgg ccgcagcgcc gcgccggccg cgaacgtgcc
     5101 gggtgccgtg aacatcgcca tcagcgtgat gaatccgccg tagctgccgc cccacacgcc
     5161 gatccggcgc ggatccacgc ggtgggcgtc ggcgaggtac ttcgcgccgt cgacgacgtc
     5221 ctcgaggtcc ttgccgccca tgtgccggta gatcgccgtc cgccagtcgc ggccgtagcc
     5281 cgcgctcgcg cggtagtcca cgtcgagcac gacgtagccc cgcgacgcca gcaggtgctc
     5341 gaacatgaac tcgcggtagt acgacgacca gtacctgtgg gcgttctggg cgtagccggc
     5401 gccgtgaacg aacacgacgc ccggcctgtt ccggtcgcgc ctggccccga tcatctccgg
     5461 cgtgaagagc cgggcgtgca cctcggcccc gtcgcgcgcc ttgaacgtga cgacctcggg
     5521 ctcgatccac ctgaaggccc gccactcctc ggtcggcgtc agcgtcacct gctgcatcgg
     5581 cgcgccggcg cggttgggca tgacgtacac ctcgggcggc ttcgtggcgg tcgaatggat
     5641 cagggcgaag accgcgccgt ccggggacgc ctctcccgtg cacgacccgg ccaccgccgt
     5701 caggcgcacg cgcggcccgc cagcgacggg catggcgtag agatggcgct cgccggggtg
     5761 cgcctcgctc gtcaccaggt ggaaggacgc tttgtcgggc gagatctggg ccgacgacac
     5821 ctcgaaccgc cccgaggtga gctgctccgg cgccgagccg ggtcgcgccg cgtccacgcg
     5881 gtaaaggtgc atccagccgt cgcgctccga caggaaccag aggctgtgcc cgtcgggcat
     5941 ccagcccagg ccgccgcccc ctctcgcgcc cgcctcgcgc acccacgcct cgtcgcgcag
     6001 ggcgtccagg acccgcgcct ttcccgtctc ggggtcgagc gc
//
LOCUS       DAOSIF010000138         6028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11861_length_6028_cov_3.137619, whole genome shotgun sequence.
ACCESSION   DAOSIF010000138 DAOSIF010000000
VERSION     DAOSIF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11861_length_6028_cov_3.137619"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLT35_06085"
     CDS             complement(<1..610)
                     /locus_tag="PLT35_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28834.1"
                     /translation="MKRKRLGVIFGGRSSEHEVSVRSASSVLARLDPSRYDTTAVYID
                     PNGRWILPSRLPDARTNAEAREFVREQSRSFRRDSEVVLPARPAADDTMLVLSRASAE
                     ERADHLATASIRHLHLDVAFPLLHGPHGEDGTIQGLFELANVPYVGAGVTASAVGMDK
                     AVAKALFRAAGIPTPRFLVVSAADWRRDRAAVLRAEESLIFFN"
     gene            779..1987
                     /locus_tag="PLT35_06090"
     CDS             779..1987
                     /locus_tag="PLT35_06090"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG28835.1"
                     /translation="MLHEEGAARRGELATAHGVVQTPAFMPVGTRGAVKAATARDLRD
                     CGAEIILANTYHLWLRPGEDLVSRLGGLHRFMGWEGPILTDSGGFQAFSLGARRAVTE
                     DGVRFRSHLDGSERLLTPERAVEIQAALGSDIAMVLDECLAQPAPLEQVRESTERSAR
                     WARRCRDRFLQLQASGAGTSRSGRTAAELPLADSPGAASVFEPLPLVTNPGQAQFGIV
                     QGGTVPALRALSAERTLAIGFEAYAIGGLSVGEPAETMYEVVGHTAPLLPANRPRYLM
                     GVGTPADIVEAVARGVDLFDCVLPTRNARNGQLFTSQGRLSIKNARFAADAGPPDPAC
                     SCYTCRTHSRAYLRHLFVAGEIGASVLNTIHNLRFYLDTMERVRQAIVFGTFELLRQS
                     VRAVYSRPVE"
     gene            1991..2347
                     /gene="yajC"
                     /locus_tag="PLT35_06095"
     CDS             1991..2347
                     /gene="yajC"
                     /locus_tag="PLT35_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOG28836.1"
                     /translation="MTLTAMFGPGLVLAMAAPAEGGANPWVQLVPFALVLAIFYFLIL
                     MPMQRKQKKVQQFLAALKVGDRVVTTSGIYGQITRISDNSVQLQIADKVRIEISRTAV
                     GGYQGQEPVASDAPGQ"
     gene            2358..3926
                     /gene="secD"
                     /locus_tag="PLT35_06100"
     CDS             2358..3926
                     /gene="secD"
                     /locus_tag="PLT35_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOG28837.1"
                     /translation="MDKNLRWRTLAILAVVGLSIWGFYPPAKKVRRGLDIQGGVHLVL
                     RVHTDDALKLETETTMERFRELLEEQGITPAGIEADGADRFKVRGVPAGQDAQFRAAA
                     NEIQTSYNRDSGSGGEYTFSLKPNLVAQLRDETVDQALQSIERRVNELGVAEPIVARS
                     GAGGDQIIVQLPGIAEPERAKAIISSTALLELKLVEDGPASTREALLQSRNGMVPSNM
                     EVVPGTSDAVAPGERPETVYYLVRKVASVTGRDLRTAKPSRDELNRPAVSFSLNQEGA
                     RKFARATGENIGRRLAIVLDGRVQSAPVIEGRIFDEGRISGGSFTQQEVQDLSLVLRS
                     GALPASLTYLEERTVGPSLGADSIRAGVIAALGGLALVVLFLLFYYRLSGVNAIVSMV
                     INLLVLLGMMAYLDATMTLPGVAGFILTIGMGVDSNVLIFERIKEELAAQRGVKQAIG
                     AAFDRVFLTILDTHVASLISAAFLFQFGTGPIRGFATTLSIGLLSNVFTSVFVSRTVF
                     DLVLSRRRVASLSI"
     gene            3930..4961
                     /gene="secF"
                     /locus_tag="PLT35_06105"
     CDS             3930..4961
                     /gene="secF"
                     /locus_tag="PLT35_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOG28838.1"
                     /translation="MEIFKKPDFDFIRWHWHALGLSAAVILAGLLFAWSRGGLPLGID
                     FKGGTIIIFKFDEAVSEEQVRAALDRMPGEKVVQKYGQASAREIMVRLPQTIEREQGA
                     LLSRDVALATEALQAANLGKLEVIGTEVVGPVIGAQLQRKGLLATVFSVMGIAAYIAV
                     RFRPSFAIGAIVATLHDVLVTIAFLAFFGYELSLNVVAAILTITGYSVNDTIVIFDRV
                     RENFRSMRRDSLAHVVNTAVNQTLSRTIITAGTTFLAGLSLFLFGGEVLEGFAFTMIV
                     GIVSGTYSTVFIAAGIAILLSRQGERAARVPAPAQAERTIRVPAQAQERTAPPAGSRK
                     KGGRKKRAS"
     gene            4981..5808
                     /locus_tag="PLT35_06110"
     CDS             4981..5808
                     /locus_tag="PLT35_06110"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG28839.1"
                     /translation="MVYLAAVALGIVQGLTEFLPVSSSAHLILARMFFGWDTGRFGLA
                     FDVACHVGTLAAVAVYFRTDLLALARAVPGLARSPAWPADGPGRRLRLIAVGSVPVAV
                     VGLLFADDIEAAVRTPWVAVASLAAIAVLFLAVERAGQGRHGEEHLSAPAALAIGVAQ
                     AAALVPGVSRSGATIATGMIAGLRRDAAAAFSFLLGVPAIGAAALHEGHDLLGRGLTA
                     GDAALFLIGMTVSAAVGFVTIKYLLRYLASHTLAAFAWYRLGLAAAAALWLLARGGA"
     gene            5805..>6028
                     /locus_tag="PLT35_06115"
     CDS             5805..>6028
                     /locus_tag="PLT35_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28840.1"
                     /translation="MIVVAWLRRRFVTGLVVTVPLALSVVTLVWLFRTVDGVVGPLSR
                     RWVPVQVPGLGLLMTAALVLGVGIVATNVLG"
BASE COUNT          819 a         2065 c         2166 g          978 t
ORIGIN      
        1 gattgaagaa tatcaggctc tcttccgcgc gaagcacggc cgcgcggtcg cggcgccagt
       61 cggcggccga aacgacgagg aaccggggtg tcgggatccc ggccgcccgg aacagggcct
      121 tcgccaccgc cttgtccatg cccacggcag acgcggtcac gcccgcgccg acgtacggga
      181 cgttggccag ttcgaacagg ccttgtatcg tgccgtcctc tccgtgaggc ccgtgcagca
      241 gcgggaacgc cacgtccagg tgcaggtgcc tgatcgaggc cgtcgctagg tggtcggccc
      301 gctcctccgc cgaggcgcgg gacaagacca gcatcgtgtc gtccgccgcc gggcgcgccg
      361 ggagcacgac ctcgctgtcc cggcggaacg agcgcgactg ctcgcgcacg aattccctgg
      421 cctcggcgtt ggttcgggcg tccgggagcc ggcttggcag gatccagcgc ccgttcgggt
      481 cgatgtagac ggccgtcgtg tcgtagcgcg acgggtccag ccgggcgagc accgaggagg
      541 cggagcgcac cgagacctcg tgttcgctgg agcgtccgcc gaagatgacg cccagccgct
      601 tgcgtttcac gacactacta tatcctgtcg gatgcgcggg ccgcgggacc cggccggcgg
      661 cccggacgag ccgcgcgggc ggcccggcgc gactcggcga ggcgcggagg agccgcctcc
      721 cgggtgtcga gtccgacgcc ctcggtactg gccaatgtct gcctcttttt ccttccgcgt
      781 gctgcatgag gagggcgctg cccggcgcgg cgaactggcg acggcccacg gcgtcgtgca
      841 gacgcccgcg ttcatgccgg tcgggacccg cggggcggtc aaggcggcca ccgcgcgcga
      901 tctccgcgac tgcggcgctg agatcatcct cgccaacacc tatcatctct ggctgcgccc
      961 cggcgaggac ctcgtctcca ggctgggagg gctgcaccgg ttcatgggct gggaaggccc
     1021 catcctgacc gacagcgggg gcttccaggc cttcagcctg ggcgcccggc gggccgtgac
     1081 cgaggacggg gtcaggttcc gctcgcacct cgacggcagc gagcggctgc tgacgccgga
     1141 gagagccgtc gagatccagg cggccctggg ctccgacatc gccatggtcc tcgacgagtg
     1201 ccttgcccag ccggccccgc tggagcaggt gcgggagtcc acggagcgct cggcgcgctg
     1261 ggcgcggcgc tgccgcgacc gcttcctcca actccaggcc tcgggtgccg gaacgagtcg
     1321 atccgggcgc acggccgcgg agctcccgtt ggccgattcc ccgggcgcag cttccgtgtt
     1381 cgaacccctt cccctcgtca ccaatcccgg ccaggcccag ttcgggatcg tccaaggcgg
     1441 caccgtcccg gcgctgcgag cgctcagcgc ggagcgaacg ctcgccatcg gcttcgaggc
     1501 ctacgcgatc ggcggcctca gcgtcggcga gcccgccgag acgatgtacg aggtcgtcgg
     1561 gcacacggcg ccgctcttgc cggcgaaccg gccgcgatac ctgatgggcg tgggcacccc
     1621 ggcggacatc gtcgaggccg tggcgcgggg cgtcgatctc ttcgactgcg tcctgccgac
     1681 gcgcaacgcc cggaacggcc agctcttcac gagccagggc cggctgagca tcaagaacgc
     1741 gaggttcgcc gccgatgccg gcccgcccga cccggcctgc tcgtgctaca cgtgccgcac
     1801 gcactcgcgg gcctatcttc gccacctgtt cgtggcgggc gagatcggcg cgtcggtgct
     1861 gaacaccatc cacaatctcc gcttctacct tgacaccatg gagcgggtga gacaggctat
     1921 agtgtttggg acgtttgagt tgctgagaca atccgtccga gccgtgtact cccgccccgt
     1981 tgagtgaacc atgaccctga cagcgatgtt cggtccgggc ctggtgctgg ccatggccgc
     2041 accggctgaa ggcggcgcaa acccctgggt gcagctcgtt cccttcgctc tcgtcctggc
     2101 catcttctac ttcctgatcc tcatgccgat gcagcgcaag cagaagaagg tccagcagtt
     2161 cctcgcggcg ctgaaggtcg gcgaccgcgt ggtgaccacc agcgggatct acgggcagat
     2221 cacccgcatc tcggataaca gcgtgcagct ccagattgcg gacaaggtcc ggatcgagat
     2281 ctccaggacg gccgttggcg gctaccaggg gcaggagccg gtcgcctcgg acgcgccggg
     2341 gcagtaacta ccaggctatg gacaagaatc tacgctggcg tacgctcgcc atcctggcgg
     2401 tcgtggggct gtcgatttgg ggcttctacc ccccggccaa gaaggtccgg cggggccttg
     2461 acatccaggg gggcgtccac ctcgtgctgc gcgtccacac cgacgacgcg ctgaagctcg
     2521 agaccgagac cacgatggag cggttccggg agctgctcga ggagcagggc atcactccgg
     2581 cgggcatcga agcggacggc gcggaccgtt tcaaggtccg gggcgtgccg gcagggcagg
     2641 acgcccagtt ccgggcggcg gccaacgaga tccagacgag ctacaaccgg gattccgggt
     2701 cgggtgggga gtacaccttc tcgctcaagc cgaacctggt ggcgcagttg cgggacgaga
     2761 cggtcgacca ggccctgcag agcatcgagc gccgcgtgaa cgagctcggc gtcgccgagc
     2821 ccatcgtcgc gcggagcggc gcggggggcg atcagatcat cgtgcagctg ccgggcatcg
     2881 ccgagccgga gcgagccaag gcaatcatca gctcgacggc gctcctcgag ctgaagctcg
     2941 tggaggacgg gccggcgagc acacgggagg cgctgctcca gagccgcaac ggcatggtgc
     3001 cgagcaacat ggaagtggtg ccgggcacga gcgacgccgt ggcgcccggc gaacgcccgg
     3061 agacggtcta ttacctggtc cggaaggtcg cctccgtcac ggggcgggac cttcggacgg
     3121 cgaagccgtc gcgcgacgaa ctcaaccggc cggcggtcag cttctcgctg aaccaggaag
     3181 gggcgaggaa gttcgcgcgg gcgacgggcg agaacatcgg ccggcgcctc gcgatcgtgc
     3241 tcgacgggcg ggtccagtcg gcccccgtca tcgaggggcg gatcttcgac gaagggcgga
     3301 tttccggggg gagcttcacg cagcaggaag tgcaggatct gtcgctcgtg ctgcggtccg
     3361 gcgcgctgcc cgcgtcgctg acctacctcg aggagcgcac cgtcggcccg agcctcggcg
     3421 ccgactcgat ccgcgcgggc gtgatcgcgg cgctgggcgg gctggcgctc gtggtgctgt
     3481 ttctcctctt ctactaccgc ctgtcgggcg tcaacgcgat cgtgtcgatg gtcatcaacc
     3541 tgctggtgct gctcgggatg atggcctacc tcgacgcgac gatgacgttg ccgggcgtcg
     3601 cggggttcat cctgacgatc ggcatgggcg tcgactccaa cgtgctcatc ttcgagcgca
     3661 tcaaggagga gctggcggcc cagaggggcg tcaagcaggc cattggggcg gccttcgatc
     3721 gggtcttcct cacgatcctc gacacgcacg tcgcgtcgct catctcggcg gcgttcctct
     3781 tccagttcgg cacgggcccg atccggggct tcgcgacgac gctgtccatc ggcctcctgt
     3841 cgaacgtgtt cacctccgtg ttcgtgtcgc ggacggtgtt cgatctggtc ctgtccaggc
     3901 gccgcgtcgc gtcattgagc atctgagcca tggagatctt caagaagccg gacttcgatt
     3961 tcatccgctg gcactggcat gccctcggcc tctcggccgc cgtcatcctg gcggggctgc
     4021 tgttcgcctg gtcgcggggc ggactgccgc tcggcatcga cttcaagggc ggcacgatca
     4081 tcatcttcaa gttcgacgag gcggtcagtg aggagcaggt gcgcgcggcg ctcgaccgga
     4141 tgcccggcga gaaggtcgtg cagaagtacg gacaggcgtc cgcgcgcgag atcatggttc
     4201 ggctgccgca gacgatcgag cgggagcagg gtgcgctgct cagccgggac gtggcgctgg
     4261 cgaccgaggc gctgcaggcg gccaacctcg ggaagctcga ggtgatcggc acggaggtcg
     4321 tcgggccggt catcggggcg caactgcagc gcaagggcct gctggcgacg gtgttctcgg
     4381 tcatgggcat cgccgcctac atcgcggtcc ggttccgccc ctcgttcgcc atcggcgcca
     4441 tcgtcgcgac gctgcacgac gtgctcgtga ccatcgcgtt cctggcgttc ttcggctacg
     4501 agctgtcgct gaacgtggtg gccgccatcc tgacgatcac cggctactcg gtcaacgaca
     4561 ccatcgtcat cttcgaccgg gtgcgcgaga atttccgctc gatgcgacgg gactcgctgg
     4621 cacacgtggt gaacaccgcc gtcaaccaga cgctgagccg cacgatcatc actgccggca
     4681 cgacgttcct cgcggggctg tcgctcttcc tcttcggcgg cgaagtgctg gaagggttcg
     4741 ccttcacgat gatcgtgggg atcgtcagcg gcacctactc gaccgtgttc atcgcggccg
     4801 ggatcgcgat tctgctgagc cgccagggcg agcgtgcggc ccgcgtcccg gccccggccc
     4861 aggccgagcg gacgatccgc gtcccggccc aggcccagga gcggaccgcg ccaccggccg
     4921 gctcccggaa gaagggcggc cgcaagaagc gagcgtccta ggttcggttc ccgactgatc
     4981 gtggtctatc tcgcggcggt ggcgctgggc atcgtgcagg ggctcacgga gttcctgcca
     5041 gtctcgagtt ccgcccacct catcctggcc cgcatgttct tcggctggga caccgggcgc
     5101 ttcggcctgg ccttcgacgt ggcctgccac gtgggcacgt tggcggccgt cgcggtctac
     5161 ttccggacgg acctgctggc gttggcgcgc gccgtgccgg ggctggctcg gtccccggcg
     5221 tggccggccg acggccccgg gcggcgcctg cggctcatcg ccgtcggctc cgtgccggtc
     5281 gccgtcgtcg ggctcctgtt cgcggatgac atcgaggcgg cggtccggac gccgtgggtg
     5341 gccgtggcgt cgctcgcggc catcgcggtg ctgttcctcg ccgtggagcg cgcgggccag
     5401 ggacgccacg gcgaggagca cctctcggcg ccggcggcgt tggcgattgg cgtggcccag
     5461 gcggccgcgc tcgtgcccgg ggtgtcgcgc tccggcgcga cgatcgcgac cgggatgatc
     5521 gccggcctgc gccgggacgc tgccgctgcg ttttcgttcc tgctcggcgt gccggccatc
     5581 ggagcggccg cgctccacga gggccacgac ctgctcgggc gcggcctcac cgccggcgac
     5641 gccgcgctct tcctcatcgg catgacggtg tcggcggcgg tggggttcgt gaccatcaag
     5701 tacctgttga ggtacctggc cagccacacc ctggcggcgt tcgcctggta ccggctcggg
     5761 ctggccgccg cggccgcgct gtggctgctc gccaggggag gggcgtgatc gtggtggcgt
     5821 ggctgcggcg gcggttcgtc accggtttgg tcgtcaccgt gccgctggcc ctgagcgtgg
     5881 tcacgcttgt ctggctgttc cggaccgtgg acggggttgt cggcccgctc tcccgccggt
     5941 gggtcccggt gcaggtgccc ggcctgggcc tcctcatgac ggcggcgctc gtcctcggcg
     6001 tcggcatcgt ggcgaccaac gtcctggg
//
LOCUS       DAOSIF010000139         6027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11866_length_6027_cov_3.898861, whole genome shotgun sequence.
ACCESSION   DAOSIF010000139 DAOSIF010000000
VERSION     DAOSIF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11866_length_6027_cov_3.898861"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLT35_06120"
     CDS             <1..390
                     /locus_tag="PLT35_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOG28841.1"
                     /translation="AGKQMAIAFETGQEEATTLAAFLTKLDRKNVGVNFDPANMILYD
                     KGDPVAALRTLAPWLRQCHLKDATRTKTPGTWGAEVRLGTGEVDWKAFFATLGDVGFT
                     GDLCIEREAGDQRVADIRAAREYVLSL"
     gene            complement(385..1155)
                     /locus_tag="PLT35_06125"
     CDS             complement(385..1155)
                     /locus_tag="PLT35_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOG28842.1"
                     /translation="MKQNFHLGIMGNLFSGKTTLVQALARDPYKRDFEALVGGAELHA
                     FSERVEKGSIVDECLALFYRDRVANVFPTEVAFLQMRTLQQREIRHLLTRESPRGVVI
                     VEDRPFMDGPEVFVARMIESGEMPPAHARLYRALFQQTLRNEHIRLPDLMVYLRTEPA
                     VLEERRAKRAAEGDDYAATIQADYLRQIHEHYEAIATTWKRTLLRYQEYATVPPDADL
                     ITLPAEIDMNEHPDYVHVAAATIREKVRRMIAARQRGL"
     gene            complement(1329..2420)
                     /locus_tag="PLT35_06130"
     CDS             complement(1329..2420)
                     /locus_tag="PLT35_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28843.1"
                     /translation="MPEITALDKHACPACGAQAEWNPGKRKLVCPFCGTESEYSFDTD
                     RSKVRELDLLQALRDLPDEARGWKAERRAVQCRSCRAVMVYDPERVGQNCEFCGSPAL
                     VDYEEIQSPIRPQGVLPFRVSQSQVRDAIRRWYASKWFAPGALKRQALVDQLKSLYVP
                     YWTFDAQVRCRWTAEAGHYYYVNEERRDGQGRARVERVRKVRWEPAAGAIDHFFDDEA
                     VSGTAGVDPGLLRGVEPFPTAEVVPYDTGYLSGHVVEHYQVALAEAAARGREAMEAQL
                     RVLCGQRVPGDTYRNLRIEPEYSGQTFKHILVPVWLLTYTFGAKTYQVVANGCTGVLA
                     GRYPKSPWKILLVVLLAVVAVAIAALAQQ"
     gene            complement(2508..3581)
                     /locus_tag="PLT35_06135"
     CDS             complement(2508..3581)
                     /locus_tag="PLT35_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG28844.1"
                     /translation="MGIMDFIKGEFIDIIEWTDDSRDTLSYRFPDEDKAIKQGAQLIV
                     RESQVAQFVYLGEFGDTFGPGKHSLTTDNIPVLTRLKSWKYGFNSPFKADVYYIATRL
                     FTGNKWGTANPVMVRDDDFGIVRLRAFGIFDFKIVEPRLFLKEVAGSDQHFRLDEFAD
                     TMRSRMVSVFSEALAASRVPVLDVATRYSELGEALLPIINPSVGSKYGLEVTSFIVEN
                     VSVPPEVEEAIDKRSSMAAIGNLNDFVKYQMAKGMQDGGSGAGGTAAELAVGFGMAQQ
                     MLQQGLMNPQAQAAMTAGGAAGAAGSAAPAVELLAPADVAKRLGVPEADVMAIITAGE
                     LKAKKIGASYRVTKAALDAYLAQ"
     gene            complement(3626..5404)
                     /gene="cydC"
                     /locus_tag="PLT35_06140"
     CDS             complement(3626..5404)
                     /gene="cydC"
                     /locus_tag="PLT35_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HOG28845.1"
                     /translation="MSRPWRFVAALARPMAWPFAAAAALQSATIACGIGLMGVSAWLI
                     ATAGLHPSIAVLGVAIVGVRFFGIARGLLRYLERLASHGATLSLLSRLRVTIYRALAP
                     RSPSGLLDERSGDLVTRLVDDVETLDAVCVRIVGPSAAAALVAALVVAVLAPRGLALA
                     GAAVAGLAVAGVLAPALARRLGARAGARSVALRADVAARAVDAVQGIGDLLAFGRGEA
                     FRADLEGRTRDLGRAQARTARVAAAASALVALAGDAAALAVLALAVPLVRAGVLDGVQ
                     LPVAMLVTLAAFEAAPPLAAAWAHLGATRAAAARIESLVGGAPAVGEPASPLPAPAGR
                     TVDVRGLTYRHPAAGRDALREVSLSLRPGRLVAVVGPSGAGKTTLAGVLLRFHDAPPG
                     TVLLDGRDVRDYRADDVRGCFAYADQRASILTGTIRENLLLGAAPSADGAPPGSDGLA
                     GAVELAGLGGLVRRLPDGLDTWIGEQGQRLSGGERQRLALARAALRAAPFLILDEPGA
                     HLDPAALRRADEALRLLARDRGVLLVTHRTIGLEGASEIVVLHEGAVIQRGTFSELRR
                     QDGWFRRMHELQRAAAAIEEPAAQAR"
     gene            complement(5401..>6027)
                     /locus_tag="PLT35_06145"
     CDS             complement(5401..>6027)
                     /locus_tag="PLT35_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG28846.1"
                     /translation="VGPSGSGKTTLAGLLLRFLEPDAGEILAGGVPLRAWARDEWLGQ
                     VAWVPQQPHVFHGTIRDNLRLARPDAGDGALAEALERARADAFVRGLPAGLDTPVGER
                     GQRLSGGEAQRIALARAFLKDAPILVLDEPTAHLDPGHEAAIRASMSELRQGRTVLLI
                     AHRLTTVADADTVAVLAGGRLVEAGTRESLLASGGRYAAMLAAYGGAA"
BASE COUNT          841 a         2231 c         2150 g          805 t
ORIGIN      
        1 gccggcaagc agatggcgat tgccttcgag accggccagg aagaggcgac gacgttggcg
       61 gcgtttctca cgaaactgga tcggaagaac gtcggcgtga acttcgaccc ggccaacatg
      121 atcctctatg acaagggcga tccggtggcc gcgctgcgca cgcttgcgcc ctggctcagg
      181 cagtgccatc tgaaggacgc cacccgcacg aagacccccg gcacgtgggg cgcggaagtg
      241 cgcctcggca cgggcgaggt ggactggaag gcgttcttcg cgacgctcgg cgacgtcggc
      301 ttcacgggcg acctctgcat cgagcgggaa gccggcgacc agcgcgtggc ggacatccgc
      361 gcggcgcggg agtacgtgct gtcgctatag gccccgctgg cgcgcggcga tcatgcggcg
      421 gaccttctcg cggatcgtgg cggccgcgac gtggacgtag tccgggtgct cgttcatgtc
      481 gatctcggcc ggcagcgtga tgagatccgc atccggcggc acggtcgcgt actcctgata
      541 gcggagcagc gtccgcttcc aggtggtggc gatcgcctcg tagtgttcgt ggatctgccg
      601 caggtagtcg gcctggatgg tcgccgcgta gtcgtcgccc tcggcggcgc gcttcgcccg
      661 ccgttcctcg agcacggcgg gctcggtccg caggtacacc atgaggtcgg gcaggcggat
      721 gtgctcgttg cgcagcgtct gctggaagag cgcgcggtac agccgggcat gcgccggcgg
      781 catctcgccg gactcgatca tgcgggcgac gaagacctcc gggccgtcca tgaacgggcg
      841 gtcctcgacg atgaccacgc cgcgggggct ctcgcgcgtc agcaggtggc ggatctcccg
      901 ctgctgcagc gtccgcatct ggaggaaggc cacctcggtc gggaagacgt tcgcgacgcg
      961 gtcgcggtag aagagagcca ggcactcgtc gacgatcgag cccttctcga cgcgctccga
     1021 gaacgcgtgc agctcggcgc cgccaacgag cgcctcgaag tccctcttgt acgggtcgcg
     1081 tgccagcgcc tgcaccaggg tggtcttgcc cgagaagagg ttccccatga tgccgaggtg
     1141 aaagttctgc ttcatgcccg tgacctcgat gcgcgcgggc gggaggcgcc cggcgttccg
     1201 cacgacagcc tagtccagcc gccgcgcccg ggcaacgggc tttgcgccgg catccgcgcg
     1261 ggcggccgcc cgatcgggag cgcgcggatg cgggcccggc gggcgccccg tgggtctggc
     1321 gcccgcgctc actgctgcgc gagcgccgcg atcgccacgg cgaccaccgc cagcaggacc
     1381 accaggagga tcttccacgg gctcttcgga tagcggccag cgaggacgcc cgtgcatccg
     1441 ttcgccacca cctggtacgt cttcgcgccg aacgtatacg tcagcagcca gacgggcacc
     1501 aggatgtgct tgaacgtctg gcccgagtac tccggctcaa tccgcaggtt ccggtaggtg
     1561 tcgccgggca cgcgctggcc gcacagcacc cgcagctgcg cctccatggc ctcccgcccg
     1621 cgcgcggccg cctcggcgag cgcgacctgg tagtgctcga ccacgtgtcc cgagaggtag
     1681 cccgtgtcgt acggcacgac ctcggccgtg gggaacggct cgacgccgcg caggaggccg
     1741 gggtccacgc cggcggtccc cgacacggcc tcgtcgtcga agaagtggtc gatggcgccg
     1801 gcggcgggct cccaccgcac ctttctcacc cgctcgacgc gcgcgcgccc ctggccgtcg
     1861 cggcgctcct cgttgacgta gtagtagtgg ccggcctcgg cggtccagcg gcagcggacc
     1921 tgcgcgtcga aggtccagta aggcacgtag aggctcttga gctgatcgac gagcgcctgc
     1981 ctcttcagcg cgcccggggc gaaccacttg ctcgcgtacc agcggcggat cgcgtcccgc
     2041 acctggctct gcgagaccct gaagggcagc acgccctggg gccggatggg cgactggatt
     2101 tcctcgtagt cgacgagcgc cggcgacccg cagaactcgc agttctggcc gacacgctcg
     2161 gggtcgtaca ccatgacggc cctgcagctc cggcactgca cggcacggcg ctcggccttc
     2221 cagccgcgcg cttcgtccgg caggtcgcgc agggcctgca gcaagtcgag ttccctgacc
     2281 ttggaccggt cggtgtcgaa gctgtactcc gactcggtgc cgcagaacgg gcagacgagc
     2341 ttgcgcttcc ccgggttcca ctccgcctgc gcgccgcagg ccgggcaggc gtgcttgtca
     2401 agggcggtga tctccggcac ggcgtcctcc cccggggccg aggcccgggg ctccatcgtg
     2461 tcgcctccgg ccgacgcggg aggctcggcc tccggccgct gcaccgctca ctgcgcgagg
     2521 tacgcgtcga gcgctgcctt cgtcacgcgg tagctcgcgc cgatcttctt ggccttcaat
     2581 tcgcccgccg tgatgatggc catcacgtcg gcctcgggca cgccgagccg cttcgcgacg
     2641 tcggcgggcg cgagcagttc gacggccggc gcggcgcttc ccgccgcgcc cgcggcgccg
     2701 ccggcggtca tggccgcctg ggcctgcggg ttcatcagcc cctgctgcag catctgctgc
     2761 gccatgccga acccgacggc cagctcggcg gccgtgccgc cggcccccga cccgccgtcc
     2821 tgcatgccct tggccatctg gtacttcacg aagtcgttca ggttgccgat ggcggccatg
     2881 ctcgaccgct tgtcgatggc ctcctcgacc tccggcggca ccgacacgtt ctcgacgatg
     2941 aagctggtga cctcgaggcc gtacttcgat ccgaccgagg ggttgatgat gggcagcagc
     3001 gcctcgccga gctcggagta cctcgtcgcg acgtccagca cgggaacgcg ggatgccgcc
     3061 agcgcctcgc tgaacacgct caccatgcgc gagcgcatcg tgtcggcgaa ctcgtcgagg
     3121 cggaagtgct ggtcggaccc ggccacctcc ttcaggaaga gcctcggctc gacgatcttg
     3181 aagtcgaaga tgccgaaggc ccgcagccgc acgatcccga agtcatcgtc ccggaccatg
     3241 acggggttcg ccgtgcccca cttgttgccg gtgaacagcc gcgtggcgat gtagtagacg
     3301 tcggccttga aaggagagtt gaagccgtac ttccaggact tcagcctcgt cagcaccggg
     3361 atgttgtccg tcgtcagcga gtgcttgccc ggcccgaacg tgtcgccgaa ttcgccgagg
     3421 tagacgaact gcgcaacctg cgactcgcga acgatgagct gcgcgccctg cttgatggcc
     3481 ttgtcctcgt cggggaagcg gtaggacagc gtgtcgcgcg agtcgtcggt ccactcgatg
     3541 atgtcgatga actcgccctt gatgaaatcc atgatcccca tgccaggctc ctctcggtga
     3601 cgaacggcga tcccgtcggc ccattctagc gcgcttgggc ggcgggctcc tcgatcgcgg
     3661 cggcggcgcg ctgcagctcg tgcatccggc ggaaccagcc gtcctgccgc cgcagctcgg
     3721 agaaggtccc gcgctggatc accgcgccct cgtgcaggac gacgatctcc gatgcgccct
     3781 ccagcccgat cgtgcggtgc gtcacgagca gcacgccgcg gtcgcgcgcc agcaggcgca
     3841 gcgcctcgtc ggcgcggcgg agcgccgccg gatcgagatg cgcgcccggc tcgtccagga
     3901 tgagaaacgg cgccgccctg agcgcggcgc gcgcgagcgc caggcgctgg cgttcgcccc
     3961 cggacagccg ctgcccctgc tcgccgatcc acgtgtccag cccgtccggc agcctccgca
     4021 cgaggccgcc gagccccgcc agctcgacgg cgcccgcaag gccgtccgat ccaggcggcg
     4081 cgccgtccgc ggacggcgct gcgccgagga ggaggttctc gcggatcgtg cccgtcagga
     4141 tgctcgcgcg ctgatcggcg tacgcgaagc atccgcgcac atcgtccgcc cggtagtccc
     4201 ggacgtcgcg cccgtcgagc agcaccgttc cgggcggcgc gtcgtggaag cgaagcagca
     4261 cgcccgcgag ggtcgtcttg cccgcgccgc tcggcccgac gacggccacc aggcgcccgg
     4321 ggcgcaaact gaggctgacc tcgcgcagcg cgtcgcggcc ggcggccgga tggcggtacg
     4381 tcaggccgcg cacgtcgacg gtgcgccccg ccggggccgg cagcgggctt gccggctcgc
     4441 cgacagcggg cgcgcctccc accagggact cgatccgggc cgccgcggcc cgcgtcgcgc
     4501 ccaggtgcgc ccacgccgcg gccagcggcg gggcggcttc gaacgccgcc agcgtgacga
     4561 gcatggcgac cgggagctgc acgccgtcca ggacgccggc ccgaaccagc ggcaccgcga
     4621 gcgcgagcac ggcgagcgcg gcggcgtcgc ctgcaagcgc caccagcgcc gacgcggccg
     4681 ctgcgacgcg ggcggtccgc gcctgggcgc gcccgagatc gcgggtgcgg ccctcgaggt
     4741 ccgcgcggaa ggcttcgccg cggccgaagg ccagcaggtc tccgattccc tgcaccgcgt
     4801 ccaccgcgcg ggcggccacg tcggcgcgca gcgcgacgct ccgtgcgccc gcgcgcgcgc
     4861 ccaggcgccg cgccagcgcc ggcgccagca cgccggcaac ggcgaggccc gcgacggcgg
     4921 cgccggcgag cgccaggccg cggggcgcga gcaccgcgac gacgagcgcc gcaacgagcg
     4981 cggcggcggc cgagggcccg acgatcctca cgcagacggc atccagggtc tcgacgtcgt
     5041 cgacgagccg ggtgacgaga tcgccgcttc gctcgtccag cagcccggac ggcgagcgcg
     5101 gcgccagcgc gcggtagatc gtcacgcgca gcctcgaaag gagcgacagc gtggctccgt
     5161 gcgaggccag gcgctccaga taccgcagca gaccccgggc gatgccgaag aaccggacgc
     5221 cgacaatcgc cacgcccagc acggcgatgg acggatggag gccggcggtc gcaatcagcc
     5281 aggccgagac gcccatcagg ccgatgccgc aggcgatggt ggcggactgc agcgccgccg
     5341 ccgcggcgaa cggccaggcc atcggccggg cgagcgccgc gacgaaccgc cacggtcggc
     5401 tcacgccgcc cctccgtacg ccgcgagcat cgcggcgtag cggccgcccg acgccagcag
     5461 cgactcgcgg gtgccggctt cgaccagccg gccgcccgcc agcacggcga ccgtgtcggc
     5521 gtcggccacc gtcgtcaggc gatgggcgat gagcagcacg gtccgccctt gccgcagttc
     5581 gctcatggac gcgcggatcg ccgcctcgtg accggggtcg agatgggccg tcggctcgtc
     5641 caggaccagg atcggcgcgt ccttcaggaa cgcgcgcgca agcgcgatgc gctgggcttc
     5701 gccgcccgac agccgctggc cccgctcccc gaccggcgtg tccagtccgg ccggcagccc
     5761 gcgcacgaag gcgtcggcgc gcgcccgctc gagcgcctcg gcaagcgccc cgtcgccggc
     5821 atccgggcgc gcgagtcgca ggttgtcccg gatcgtcccg tggaacacgt gcggctgctg
     5881 cgggacccag gccacctgcc ccagccactc gtcacgggcc caggcccgca gcggcacccc
     5941 gcccgccagg atctcgcccg cgtcaggctc gaggaagcgc agcaggagcc cggccagcgt
     6001 ggtcttgcca gagccgctcg ggccaac
//
LOCUS       DAOSIF010000140         6014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_11900_length_6014_cov_4.622420, whole genome shotgun sequence.
ACCESSION   DAOSIF010000140 DAOSIF010000000
VERSION     DAOSIF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11900_length_6014_cov_4.622420"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1360)
                     /locus_tag="PLT35_06150"
     CDS             complement(65..1360)
                     /locus_tag="PLT35_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOG28847.1"
                     /translation="MNTASSRGNRWLVLAGGVLVQLAIGAVYAWSTFSKALQAESSAF
                     GLSKVQAAIPFEVAIGMIFVGAFLGGRVQDRRGPRLVAVVGVTLYSLGIILASFARGA
                     GGLWLLVLGYGVLGGFGLGLAYIVPIAMLQKWFPDKRGLITGVAVGGFGFGAVITSPL
                     AQAIIDSSEIYQRCPTRVFLWLGLAYLVAGLAGTSVFRNPPEDHAAPRRAAASGASAA
                     ARASSGGAANAATVPAAAGRDFSQAEALRTVQWYLLTLILTISVTAGISFISVAAGTA
                     SDVAGFSAAGAATLVGVMGLFNGGGRILWAAISDRIGRKRAFVGILALQGLALLAIPH
                     ADNAVLFCVLAALIYACYGGGFGTMPSTASDFFGVRHAGAIYGLMLIGWSIGGIVGPL
                     LIAWLIGDGQAYTRGFTAVGALALVGAVLPYAARPPRRS"
     gene            1643..2086
                     /locus_tag="PLT35_06155"
     CDS             1643..2086
                     /locus_tag="PLT35_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOG28848.1"
                     /translation="MRTHRVATAVLVALLAVPAAALAQGQSDEDAIKALVQTAYVDGL
                     QNLGDLDRTRAGFHADFVLLGLRDGALTRLPIDEWIASAAKRKADGVKPPLTTCKFLN
                     VDVTGSAASVKLELHQNDRRIFTDYLSLYKFPDGWKIVGKIYYRH"
     gene            2236..2415
                     /locus_tag="PLT35_06160"
     CDS             2236..2415
                     /locus_tag="PLT35_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28849.1"
                     /translation="MFDTGETPDYPLFHEPQYVISLATRIVQVAREGRRAVASRYCAN
                     EIVPRHEALRREPGA"
     gene            complement(2451..4817)
                     /locus_tag="PLT35_06165"
     CDS             complement(2451..4817)
                     /locus_tag="PLT35_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28850.1"
                     /translation="MTSRREFLKGVAAGMALIGSAPSATPLAAARPQGLSASDLPEGG
                     APAPLVPAHFPDRLHAFVWRNWPLVPAEVMARVVGASASQIRDLGRSMGLGDQPAISL
                     DQQMRSRLTVIRRNWHLLPYAQLLDVLGWTAEQLDYALREDDFLFIKLGRLKPRCGPL
                     RYAPPGRAAAERAREIARVLRDECPADSPEGADPLFGFVSRLSRKPAAAEPADRRAPG
                     DGLRFCYSYFALYGDPLLDPEPDPYPDGYLARLAQAGVNGIWLQAVLYKLAPFPWEPQ
                     RSAGHRTRLENLRTLVDRAARHGIRVLLYLNEPRTMPERFFEGRPGLKGVSSGGYAAL
                     CTSVPEVQRYLVDSVASICRAVPELGGFFSITASENPTNCWSHGAGAACPRCGARSPA
                     EVIAEVNCLFAEGIRASGSAARLIAWDWGWNDAWAGDAIGRLAAGTALMSVSEWRLPI
                     ERGGVTTEVGEYSISAVGPGPRARAHWNAAAGHKLETVAKIQAGNTWELSSVPYIPAV
                     ENVARHAARIRESGVSGVMLGWTLGGCPSPNLEVVSEALACGSADEAMSRVARRRFGP
                     RLGPLVVEAWRRFSAAFSEYPYHGEVLYNGPQQLGPANQLWGEPTNYRATMTGFPYDD
                     LDAWRAVYPPDVFIRQFDKMADGFDSALAELRVSAREAARAASASEREALAEEERVAE
                     AAAIHFRSTANQARFVLARRNVAEAKAPSEAAPHLDALERVLEDEIALARRLRRIQLS
                     DSRFGFEASNQYFYVPVDLQEKILNCRDLLTRWLPQLRAAAGAVPAAE"
     gene            complement(4814..6001)
                     /gene="odhB"
                     /locus_tag="PLT35_06170"
     CDS             complement(4814..6001)
                     /gene="odhB"
                     /locus_tag="PLT35_06170"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOG28851.1"
                     /translation="MVPEVGESVVDARVVRWLKQPGEAVAAGEAVVELETDKVDVEVA
                     APRAGVLGRIDHADGADVKVGDVLGVIDDSEAGAAAVPPARAEAAPPATAATPPGAAR
                     ADRARATPAARRAADAHDVDLARVRGSGPGGRIMRRDVEDAAPSASASQPSRQPSAER
                     ARGERAEERIRMSKRRATIARRLVEAQHTAAMLTTFNEADMSAVMALRERHKQAFRER
                     HGVGLGIASFFVKAAIGALREFPRLNAEIQGDEIVLKRYYDIGVAVGATEGLVVPVLR
                     DADRLSFAEIEREIRRFAAAAEDGSLSLADLQGGTFTITNGGVFGSMLSTPILNPPQV
                     GILGLHAIKERPVGIGGQVVLRPLMYLAMTYDHRIVDGAEAVRFLVRVKELVEDPGAM
                     LLE"
BASE COUNT          905 a         2187 c         2153 g          769 t
ORIGIN      
        1 cgtcggggcg cgggcgcgcg cggagcggcc gcgtcgcgac cggcccgtcc gcgcgctgga
       61 gcgatcagct gcgcctgggg ggccgcgcgg cgtacggaag gacggcgccg acgagggcga
      121 gggcaccgac tgccgtgaag ccccgtgtgt aggcctggcc gtcgccgatg agccaggcga
      181 tgagcagcgg gccgacaatc ccgccgatcg accacccgat gagcatgagg ccgtagatgg
      241 cacccgcgtg gcgcacgccg aagaagtcgc tcgccgtcga gggcatggtg ccgaagccgc
      301 cgccgtaaca ggcatagatg agggccgcaa gcacgcagaa cagcaccgcg ttgtccgcat
      361 gagggatggc cagcagcgcc agcccctgga gcgccaggat cccgacgaag gcccgtttgc
      421 gcccgatccg atccgagatc gccgcccaca ggatgcggcc gccgccgttg aacagcccca
      481 tgacgccgac cagcgtcgcc gccccggcgg cgctgaagcc cgccacgtcg ctggcggtcc
      541 cggccgccac cgagatgaac gaaatgccgg ccgtcaccga gatggtcagg atcaaggtca
      601 gcaggtacca ctgcacggtg cgcagcgcct ctgcctggga gaagtccctg cccgccgcgg
      661 cgggaacggt ggcggcattc gcggcgccgc cactcgacgc acgggccgct gccgacgccc
      721 cggaggcagc ggcgcgcctc ggcgccgcgt gatcctcagg agggttgcgg aaaaccgacg
      781 tcccggccag cccggcgacc aggtaggcca ggccgagcca caggaagacc ctggtggggc
      841 agcgctggta gatctcgctg ctgtcgatga tcgcctgcgc cagcggcgag gtgatcacgg
      901 cgccgaagcc gaagccgcca accgcgaccc cggtgatcag cccgcgcttg tcgggaaacc
      961 acttctgcag catggcgatc ggcacgatgt aggccagccc caggccgaag ccgccgagca
     1021 cgccataccc gaggacgagc agccacaggc cgccggcgcc gcgggcgaag ctggccagga
     1081 tgatcccgag ggaatagagc gtcaccccga cgacggcgac cagccggggg ccccgccgat
     1141 cctggacgcg gccgccgagg aaggcgccga cgaaaatcat cccgatggcg acctcgaagg
     1201 gaatggccgc ctgcaccttg gacaggccga aggccgagga ctcggcctgc agcgccttgc
     1261 tgaacgtgct ccacgcgtag acggcgccga tcgcgagctg cacgagcacg ccgccggcga
     1321 gaacgagcca gcggttcccc cttgatgacg cagtgttcat cgcccctcct gggccctgaa
     1381 tcgtccaatc accgtacggt gacgccgctg ccgtccgcgc cgccgcgcca gccggggcgc
     1441 cacgtggacg cagcgcccat tgtcccgccg cgccggagcg gatcaacccg gtgccccggc
     1501 gattcggtcg cggcgcagga acccgcgccg agccggcggt cgagggccgt cacggccggg
     1561 attctaccgc gcctggcagc ccgtgttggc ggaccggcgc gaggcggtgt acgttatccg
     1621 gaagtcccca tcaggaggag gcatgcgcac acaccgcgtc gccacggccg tgctcgtcgc
     1681 gctgctcgcc gttccggcag ccgcgctcgc ccagggccag tccgacgagg acgcgatcaa
     1741 ggccctcgtg cagaccgcct atgtggacgg cctgcagaac ctcggcgacc tggacagaac
     1801 cagggcaggc tttcacgccg acttcgtcct gctcgggctg agagacggcg cgctcacgcg
     1861 tcttcctatc gacgagtgga tcgcctcggc cgcgaagcga aaagccgacg gggtgaagcc
     1921 gccgctgacg acctgcaagt tcctgaacgt cgacgtgacc gggtcggccg cgtccgtgaa
     1981 gctggaactc caccagaacg acaggcggat cttcaccgac tacctgtcgc tgtacaagtt
     2041 ccccgacggc tggaagatcg tcggcaagat ctactaccgg cactgaccct cggtgaacgc
     2101 cgcggccccc atgcccgggg tcaccgcgac aacgaccggc gaatgcctgc cggcgctgcc
     2161 ggccggcacg cgcgcccgag cgcacatgcc cgggcaggcg ctggcagtcc gctgcgcgcc
     2221 gatcccggcg gcgtcgtgtt cgatacgggc gagacgcccg actacccgct gtttcacgag
     2281 ccgcagtacg tgatctcgct ggccacgcgg atcgtgcagg tggcccgcga aggccgccgg
     2341 gcggtggcct cccgttattg cgccaacgag atcgtgccgc ggcacgaggc gctgcgccgc
     2401 gaaccgggcg cctgagcgcc ctcggcccgg cgggttcgcc cgcgagcgcc tcactccgct
     2461 gccggaacgg ctccggcggc cgcccgaagc tgcggcagcc agcgcgtcag caggtcgcgg
     2521 caattcagga tcttctcctg cagatcgaca ggaacgtaga agtactggtt cgaagcctcg
     2581 aagccgaagc gggaatccga gagctggatg cggcgcagcc ggcgcgccag cgcgatttcg
     2641 tcctcgagca cgcgctccag ggcgtccagg tgcggcgcgg cctcggacgg ggccttggcc
     2701 tccgcgacgt tccgacgggc caggacgaac cgcgcctggt tcgccgtgct tcggaagtgg
     2761 atggcggccg cctcggccac gcgctcctcc tcggccagcg cctcccgctc ggaggccgac
     2821 gcggcccgtg cggcctcgcg cgccgacact ctcagctctg ccagggccga gtcgaagccg
     2881 tccgccatct tgtcgaactg gcggatgaag acgtccgggg gatagacggc gcgccacgcg
     2941 tccaggtcgt cgtacgggaa acccgtcatc gtggcgcggt agttcgtagg ttcgccccag
     3001 agctggttgg cggggccgag ctgctgcggg ccgttgtaga gcacctcgcc gtggtacggg
     3061 tactcgctga aggcggcgct gaacctgcgc cacgcctcga cgaccagcgg cccgagccgc
     3121 gggccgaacc ggcggcgggc cacgcggctc atcgcctcgt cggcagaccc gcaggcgagc
     3181 gcctcggaca ccacctccag gttgggagac gggcagccgc ccagcgtcca ccccagcatc
     3241 acgccgctca cgccgctctc gcggatgcgc gccgcgtggc gcgcgacgtt ctcgacggcg
     3301 gggatgtacg ggaccgagga gagctcccag gtgttccccg cctggatctt ggcgaccgtc
     3361 tcgagcttgt gccccgcggc cgcgttccag tgagcgcggg cgcgcgggcc gggtccgacg
     3421 gcggaaatcg agtactcccc gacttccgtc gtgacgccgc cgcgctcgat gggcagccgc
     3481 cactcgctga cgctcatcag cgcggttccc gcggccagcc ggccgatggc gtcgccggcc
     3541 cacgcgtcgt tccagcccca gtcccacgcg atgagccggg cggccgagcc cgatgcccga
     3601 atgccctcgg caaacaggca attcacttcg gcgatgacct cggcaggtga ccgggcgccg
     3661 caccgcgggc acgcggcgcc ggcgccgtgc gaccagcaat tcgtcgggtt ctccgaagcg
     3721 gtgatgctga agaacccgcc gagctccggg acggccctgc agatcgacgc gacggagtcg
     3781 acgaggtacc gctggacctc gggcacgctg gtgcacagcg cggcgtagcc gccgctgctg
     3841 acgcccttca gcccgggccg gccctcgaag aaccgctcgg gcatcgtccg gggctcgttc
     3901 aagtacagaa ggacccggat gccgtggcgg gcggcacggt cgaccagcgt ccgcaggttc
     3961 tcgaggcggg tccgatggcc cgcgctgcgc tgcggctccc acgggaacgg cgccagcttg
     4021 tagaggaccg cctggagcca gatgccgttc acgcccgcct gggcgagccg agccaggtag
     4081 ccgtccggat acggatcggg ctccggatcg agcaggggat cgccgtagag ggcgaagtag
     4141 gagtagcaga acctgagccc gtcgccgggc gctcggcgat cggccggctc ggccgcggcc
     4201 ggcttccgtg agaggcgcga gacgaagccg aagagcgggt cggcgccctc cgggctgtcg
     4261 gcggggcatt cgtcgcgcag gacgcgggcg atctcgcgcg cccgctccgc ggccgctcgt
     4321 cccggcggcg cgtaccgcaa gggcccgcac ctgggcttga ggcggccgag cttgatgaac
     4381 aggaagtcgt cctcgcgcag ggcgtagtcg agctgctccg ccgtccagcc cagcacgtcg
     4441 agcaactggg cgtagggcag caggtgccag ttgcggcgga tgacggtgag ccgcgatcgc
     4501 atctgctggt cgaggctgat cgcgggttga tcgcccagcc ccatcgatcg gcccaggtcc
     4561 cgaatctggg aggcgctggc gccaacgacg cgggccatca cctcggcggg aaccaggggc
     4621 cagtttcgcc agacgaacgc gtggagccga tccggaaagt gggccgggac gagcggcgcg
     4681 ggcgctcccc cttccggcag gtcggacgcc gacaggcctt gcgggcgggc cgccgccaag
     4741 ggcgtggcgg agggcgccga cccgatcagc gccatgcccg cggccacgcc cttgagaaac
     4801 tcgcgccgcg acgtcattcg agcagcatgg cgccagggtc ctcgacgagt tccttgacgc
     4861 gaaccaggaa gcgcacggct tcggcgccgt ccacgatgcg gtggtcgtag gtcatcgcca
     4921 ggtacatcag cgggcgaagg acgacctgac cgccgatgcc gaccgggcgc tccttgatgg
     4981 cgtgcaagcc gaggatgccg acctgcggcg ggttgaggat cggcgtgctg agcatcgatc
     5041 cgaacacccc gccgttggtg atggtgaacg tgcctccctg caggtccgcc agcgacagcg
     5101 acccgtcctc ggcagccgcc gcgaaccggc ggatctcccg ctcgatctcg gcgaacgaga
     5161 gccgatcggc gtcgcgcagc acggggacga ccagcccctc ggtcgcgcca acggccacgc
     5221 cgatgtcgta gtagcgcttc aggacgatct cgtcgccctg gatctcggcg ttcagccgcg
     5281 ggaactcgcg cagcgcgccg atggccgcct tgacgaagaa cgaggcgatg ccgaggccga
     5341 cgccgtgccg ctccctgaag gcctgcttgt gccgttcgcg cagcgccatg acggccgaca
     5401 tgtccgcctc gttgaacgtg gtgagcatgg ccgcggtgtg ctgcgcttcg accaaccggc
     5461 gcgcgatggt cgcgcgccgc ttcgacatgc ggattcgctc ttcggcccgc tcgccgcggg
     5521 cgcgctcggc ggagggctga cgagacggct gggaggcgga cgcggacggc gcggcgtctt
     5581 cgacgtcccg gcgcatgatc cgcccgccgg gtccgctgcc gcgcacccgc gcgagatcga
     5641 cgtcgtgcgc gtccgcggcc ctgcgggccg ccggcgtcgc gcgggccctg tcggcccgcg
     5701 cggcacccgg cggcgtcgca gccgtcgctg gaggcgcggc ctcggcccgg gccggaggca
     5761 cggcggcggc tcccgcctcg ctgtcgtcga tgacgcccag cacgtcgccc accttcacgt
     5821 cggcgccgtc cgcgtgatcg atccggccga ggacgccggc cctcggcgcc gccacttcca
     5881 cgtccacttt gtcggtctcg agctcgacca cggcctcgcc ggcggccacg gcctcgccgg
     5941 gctgcttgag ccaccggacg acgcgagcgt ccacgaccga ttctccgact tccggcacca
     6001 cgatgtctgc ggcc
//
LOCUS       DAOSIF010000141         5930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12112_length_5930_cov_5.115745, whole genome shotgun sequence.
ACCESSION   DAOSIF010000141 DAOSIF010000000
VERSION     DAOSIF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12112_length_5930_cov_5.115745"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="PLT35_06175"
     CDS             <1..1369
                     /locus_tag="PLT35_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="coagulation factor 5/8 type domain-containing
                     protein"
                     /protein_id="HOG28852.1"
                     /translation="AEGLSVHPPDGVVQWPVSQAAPFRRMRVRGDLVLHQGLGFASGG
                     WMSDTAVDGLVNAGSQQQSIARNCEWEGWKGANWNMVFVGTLNAPRGEWPGAAITRID
                     KTPLVREKPFLMIDTRERWVVRVPGLRADSSGVSWQMGLTPGRYIPLGRFHIARPGAD
                     TAATINAQLARGRHVIFTPGTYRLRETIRVTKPDTVVMGLGFATLLAEGGVPAMSTAD
                     VDGIVVSGLVFDAGADKVPVLLDVGPPKSRARHVANPASLHDVFFRVGGAARGRASVC
                     LRVNAHDTIVDHAWIWRADHGAGVGWTGNVSANGIVVNGSHVTVYGLFVEHHQEFQTL
                     WNGEAGRVYFYQSEVPHDPPDQASYSSSPGTNGWAAYKVADGVTRHEAWGLGVYSVFS
                     RPGIALTRAFEVPSAPGVMLRHLVTACIGPNGEISNVVNDEGGATLPNAERMPRVTAY
                     PAPGR"
     gene            1416..2813
                     /locus_tag="PLT35_06180"
     CDS             1416..2813
                     /locus_tag="PLT35_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG28853.1"
                     /translation="MANPDTSGARQGMFTVEGGKSVAVTFALVSSLFLLWGLCNGMID
                     VMDKHFQTELGLSKSQSAWVQFAHYLGYFLMALPAGWLATRLGYKGGIIAGLLMVAAG
                     GFWFVPATRINAMAQAGDVSHAAAFVGYLAGVCAIAAGLTFLETVANPYTTVLGPKQY
                     AATRINLAQSCNGVGWIFGPIIGGIFFYSTDGSGRSTGSETLYIPYLVVALVVLVLAV
                     VFYVADIPDIKAEDDYHLDNAAPGMSHALWRRPHFVMAVAAQFLYVAAQAGIFSFFIN
                     YMTEEVPAIPASWGAGALGPWLEAGAGGGLRISDQGAANLASLGFVCFLTGRFTGAGL
                     LRKVAAHKVLGLYGLLNVAACLLVFLKLGWLSVACVFLSYFFMSIMFPTIFALGIFGL
                     GARAKQASAFIVMAIVGGALLPKVMGRVADLYDMSRGFAVPLLCFAFVAAYGCLWPRL
                     SGAPSMTVTQAGGRD"
     gene            2824..4107
                     /locus_tag="PLT35_06185"
     CDS             2824..4107
                     /locus_tag="PLT35_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG28854.1"
                     /translation="MKPIRHLRWYIGGLFFLSTVINYIDRQTLSVLAPYLKTQFAWTN
                     ADFALIVISFRVAYAVGQTGAGQVIDRVGTRTGLSLGVAFYSTAAMLTSLACGLRSFC
                     FFRFLLGAGEAANWPGATKGVSEWFPRRETGWAVALFDSGSSIGGAVAPVLVLWIFHT
                     FGGWQPAFIITGTLGFAWLLLFRWFYRSPETHPRLSAEERAYILADRPAPQADAGAGP
                     AHRIPVRTLLRLPQTWGVIIAKTLSDPVWFFITDWFAIFLVSRGFRLESSLFAFWVPF
                     LAADAGNFLAGGVSSTLIKRGWAVGTARKTVIVVCALGMTLIAAAAYVSSLWWITAFF
                     AVSTLCYAALSTMVLNLPADIYPSHSVASVSGMSGTGAGIGTIVATYLTGVVADRYSF
                     EPILVVASLVPLVAAAAVLGLVRNRRETRGMLNEI"
     gene            complement(4235..5002)
                     /locus_tag="PLT35_06190"
     CDS             complement(4235..5002)
                     /locus_tag="PLT35_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOG28855.1"
                     /translation="MIVKRFDTRPSMARAAAAHAALAIRQAVAARGRARIVVATGASQ
                     IEFLQALTADPSVDWARVALFHLDEYVGLPITHPASFRKYILERVIRPVSLSDYLLLD
                     GEGDPAEVCRQAGAAIAASPVDVAFVGIGENGHLAFNDPPADFVTEEPYLVVQLDEAC
                     RRQQVGEGWFASVAEVPASAISMSVRQILKAREIICVVPDARKAVPVKAALEGPVTPD
                     VPASALREHANVTVYLDADSAALLEADALARFAPPVA"
     gene            5212..5484
                     /locus_tag="PLT35_06195"
     CDS             5212..5484
                     /locus_tag="PLT35_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28856.1"
                     /translation="MMSALDAGWAEEPFAIKDGKGVPRERRSVLDEIQARDLRQVPGM
                     SHDEAAPSMTAAVAIRRPAVPTDLPAARSLANSLPCPAGVPDYRND"
     gene            complement(5481..>5930)
                     /locus_tag="PLT35_06200"
     CDS             complement(5481..>5930)
                     /locus_tag="PLT35_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28857.1"
                     /translation="GFRGFGDNADGGLTALALAAGVGIQFDSVADLQPYTVWTESGRE
                     DSRCMIGGWSAFVGLRTPAIPVGGGSSVRAGVNRGRLAVVAERGVTWLGETACEDCPS
                     ERLGFRGGGYFEYYLAFQRESGARCLIAYRTYAAESDLRNSVIVGVG"
BASE COUNT          820 a         2125 c         2058 g          927 t
ORIGIN      
        1 agccgagggg ctctcggtgc acccgccgga cggcgtggtg cagtggcccg tgtcgcaggc
       61 cgcgcccttc cgccgcatgc gcgttcgcgg cgacctggtg cttcaccagg gcctcgggtt
      121 cgcaagcggc ggctggatga gcgacacggc cgtcgacggc ctcgtcaacg cgggctccca
      181 gcagcagtcg atcgcgagga actgcgagtg ggagggctgg aagggcgcga actggaacat
      241 ggtgttcgtc ggcacgctca acgcgccccg aggcgagtgg cccggggcgg ccatcacccg
      301 gatcgacaag accccgctcg tccgcgagaa gccgttcctc atgatcgaca cgcgggagcg
      361 ctgggtggtg cgcgtgcccg gccttcgagc ggattcgtcg ggggtcagct ggcagatggg
      421 gctgacgcca ggccggtaca tcccgctcgg ccgcttccac atcgcccgcc ccggcgcgga
      481 cacggccgcg acgatcaacg cgcagctggc caggggccgc cacgtcatct tcacccccgg
      541 aacctaccgc ctgcgggaga ccatccgggt cacgaagccc gacacggtgg tgatgggcct
      601 cgggttcgcg acgctcctgg ccgagggcgg cgtgccggcg atgtccaccg cggacgtgga
      661 cggcatcgtc gtctccggcc tcgtcttcga cgccggcgcc gacaaagtgc ccgtcctgct
      721 cgacgtcggg ccgccgaaga gccgcgcgcg gcacgtggcg aacccggcct cgctgcacga
      781 cgtgttcttc cgggtcggcg gcgccgcacg cggccgggcc tccgtctgcc ttcgcgtcaa
      841 cgcgcacgat acgatcgtcg atcacgcgtg gatctggcgc gcggaccacg gcgccggcgt
      901 gggctggacc ggcaacgtca gcgccaacgg catcgtcgtc aacggcagcc acgtcaccgt
      961 ctacggcttg ttcgtggagc accaccagga gttccagacg ctgtggaacg gcgaggccgg
     1021 ccgggtctat ttctaccaga gcgaggtccc gcacgacccg ccggaccagg ccagctactc
     1081 gagctcgccg ggcacgaatg gctgggccgc ctacaaggtc gccgacgggg tcacccgcca
     1141 cgaagcctgg ggcctggggg tgtacagcgt gttcagcagg ccgggcatcg ccctgacgcg
     1201 ggcgttcgag gtgcccagcg cgcctggcgt gatgctgcgc cacctggtca ccgcctgcat
     1261 cgggccgaac ggcgagatca gcaacgtcgt caacgacgag ggcggagcga cgcttcccaa
     1321 cgcggagcgg atgccgcggg tcaccgccta tcccgccccc gggaggtgac gcggcgctcg
     1381 cggatcgcgt ttcggaggcc tcttcaggag atgtgatggc gaacccggac acatcaggcg
     1441 cgcggcaggg catgttcacg gtcgagggcg gaaagagcgt ggcggtcacc ttcgccctcg
     1501 tcagctcgct gttcctgctc tgggggctgt gcaacggcat gatcgacgtg atggacaagc
     1561 acttccagac cgagctcggt ctgtcgaagt cgcagtccgc ctgggtgcag ttcgcccact
     1621 acctggggta cttcctgatg gcgctgccgg ccggctggct ggcgacgcgg ctgggctaca
     1681 agggcggcat catcgccggg ctcctgatgg tcgcggccgg gggcttctgg ttcgtcccgg
     1741 cgacccggat caacgcgatg gcgcaggccg gtgacgtgtc gcacgcggcg gcgttcgtgg
     1801 gctacctggc cggcgtctgc gcgattgcgg ccggcctcac gttcctcgag accgtcgcca
     1861 acccgtacac cacggtgctc ggcccaaagc agtacgccgc cacgcgcatc aacctcgcgc
     1921 agtcgtgcaa cggcgtgggc tggatcttcg ggccgatcat cggcgggatc ttcttctact
     1981 cgaccgacgg ttccgggcgc agcacgggca gcgagacgct gtacatcccc tacctcgtcg
     2041 tcgccctcgt cgtcctcgtg ctggcggtgg tcttctacgt cgccgacatc ccggacatca
     2101 aggccgagga cgactaccac ctcgacaacg cggcgccggg gatgtcccac gcactctgga
     2161 ggcgcccgca cttcgtgatg gccgtggcgg cgcagttcct gtacgtcgcg gcgcaggccg
     2221 gcatcttcag cttctttatc aactacatga cggaggaagt gcccgccatc cccgcgtcgt
     2281 ggggggccgg cgcgctcggg ccgtggctcg aagccggagc cggaggcggc ctgcggatca
     2341 gcgaccaggg cgccgccaac ctcgcctcgc tcggcttcgt ctgcttcctg acgggccggt
     2401 tcacgggcgc ggggctgctg cggaaggtcg cggcgcacaa ggtgctcggc ctctacgggc
     2461 tcctcaacgt cgccgcgtgc ctcctggtct tcctgaagct gggctggctt tccgtcgcct
     2521 gcgtgttcct gagctatttc ttcatgtcga tcatgttccc gacgatcttc gccctcggca
     2581 tcttcggcct cggcgcgcgc gcgaagcagg cgtccgcctt catcgtcatg gccatcgtgg
     2641 gcggcgcgct cctgccgaag gtgatgggcc gcgtggccga cctctacgac atgtcgcgcg
     2701 gcttcgccgt gccgctgctg tgcttcgcgt tcgtggccgc gtacggctgc ctctggccga
     2761 ggttgagcgg ggcgccgtcg atgaccgtga cgcaggccgg cggccgggac tgaggacttc
     2821 agcgtgaaac ccatccgaca cctgcggtgg tacatcggag gcctgttctt cctctccacc
     2881 gtcatcaact acatcgaccg acagacgctc agcgtcctgg cgccgtacct gaagacccag
     2941 ttcgcctgga cgaacgcgga cttcgcgctg atcgtgatct cgttccgggt cgcgtacgcc
     3001 gtcggccaga ccggagcggg ccaggtgatc gaccgcgtcg gcacgcggac cgggctgagc
     3061 ctcggcgtgg ccttctactc gacggccgcc atgctcacgt cgctggcctg cggcctgcgg
     3121 tcgttctgct tcttccgctt cctgctgggc gccggcgagg cggccaactg gccgggcgcc
     3181 acgaaggggg tgtcggagtg gttcccgcgc cgcgaaaccg gctgggccgt ggcgctgttc
     3241 gacagcggat cgtcgattgg cggcgccgtg gcgcccgtgc tggtgctgtg gatcttccac
     3301 acattcggcg gctggcagcc ggcgttcatc atcaccggca ccctcggctt cgcctggctg
     3361 ctgctgttcc gatggttcta ccgatcgccg gaaacgcacc cccgcctctc ggccgaggag
     3421 cgggcctaca tcctggcgga ccgccccgcg ccgcaggccg acgccggcgc cggcccggcc
     3481 catcgcatcc cggtccgcac gctcctgcgc ctgccccaga cgtggggggt catcatcgcc
     3541 aagacgctca gcgatcccgt ctggttcttc atcaccgact ggttcgccat ctttctcgtg
     3601 tcgcgcggct tcaggctcga aagcagcctg ttcgcgttct gggtcccatt cctggcggcc
     3661 gacgccggca acttcctcgc cggcggcgtg tccagcaccc tcatcaagcg cggctgggcg
     3721 gtgggcacgg cgcgcaagac ggtgatcgtc gtctgcgcgc tgggcatgac gctcattgca
     3781 gccgcggcgt acgtctcgag cctctggtgg atcaccgcgt tcttcgccgt gtccaccctg
     3841 tgctacgccg cgctctccac gatggtcctg aacctgccgg ccgacatcta cccgagccac
     3901 tcggtggcgt cggtgagcgg aatgagcggc acgggcgccg gcatcgggac gattgtggcc
     3961 acctacctga cgggcgtcgt cgcggaccgc tactcgttcg agccgattct cgtggtggcc
     4021 agcctcgtgc cgctcgtcgc cgcggcggcg gtcctggggc tcgtccggaa caggcgcgag
     4081 acgcggggaa tgctcaacga aatctagcgc gggctgcggc ccgggcccca ggatccagcc
     4141 gcccgatctc cacggcaccc gggatctccg ccgctggcga agccccctcc cgccgaaccc
     4201 gacggagccg ccgagcggac cgggcccggg gccttcaggc cacgggcggc gcgaatcggg
     4261 cgagcgcgtc cgcctcgagc agggcagccg agtcggcgtc caggtacacc gtcacgttcg
     4321 cgtgctcgcg cagcgccgac gccgggacgt ccggcgtgac cgggccctcc agcgctgcct
     4381 tgacgggaac ggccttccgc gcgtcgggca cgacgcagat gatctcgcgc gccttcagga
     4441 tctgccggac cgacatcgag atcgccgatg cggggacctc cgcgacggat gcgaaccagc
     4501 cctcgccgac ctgctgccgg cggcacgcct cgtccagctg gaccacgagg tagggctcct
     4561 cggtgacgaa gtccgccggc gggtcgttga aggcgaggtg tccgttctcg ccgatgccca
     4621 cgaacgcgac gtcaacgggc gaggcggcga tggcggcccc ggcctggcgg cacacctcgg
     4681 ctggatctcc ctcgccgtcc agcagcaggt agtccgacag cgaaacgggc cggatcacgc
     4741 gctccagaat gtacttcctg aagctggccg ggtgggtgat cggcaggccc acgtactcgt
     4801 cgagatgaaa gagcgccacc cgcgcccagt ccaccgacgg atccgccgtc agggcctgga
     4861 ggaactcgat ctgggacgcg ccggtggcga cgacgatgcg ggctcgaccg cgggcggcga
     4921 cggcctggcg gatcgccaac gccgcgtgcg cggcggcggc ccgggccatg gaaggcctgg
     4981 tgtcgaaacg cttgacgatc atgggtgtgt cacttcctcg cgcgggaggc ggcccgcgga
     5041 ccgcggggtg cacgtttcca gcattatcgc ccgccgccgg ggccctggat cggcctcgag
     5101 ccgcttcgct tccggtgtgg cgcccgagcc tgcccgcacg agggcccgcc gctcggtcca
     5161 ctcggccgcg gcctcatcca cccggcgcta gcgaacgcgg gtgccgcacg cgtgatgtcg
     5221 gcgctcgacg cgggctgggc ggaggagccc ttcgccatca aggacgggaa gggcgtgccg
     5281 cgagagcgac ggtcagtgct cgacgagatc caggcccggg accttcgcca ggtgcccggt
     5341 atgtcgcatg acgaggccgc gccgtccatg acggccgccg tcgcaatcag gagacccgcc
     5401 gtgccgacgg acctccccgc cgctcgcagc ctggcgaaca gcctcccgtg cccggctggc
     5461 gtgccggact accggaacga ctagccgacg ccaacgatga cgctgttgcg gaggtcgctc
     5521 tcggccgcgt acgtgcggta ggcaatcagg caccgagcgc cgctctcgcg ctggaacgcg
     5581 aggtagtact cgaaatagcc gccgcctcga aatcccagtc tctccgacgg gcagtcctcg
     5641 cacgcggtct ccccgagcca ggtgaccccg cgttctgcga cgaccgcgag gcggcccctg
     5701 ttcacgccgg cgcgcaccga cgagcccccg ccgacgggga tggccggcgt cctcaggccc
     5761 acgaaagccg accacccgcc gatcatgcag cggctgtctt cccttccgct ctcagtccag
     5821 acggtgtacg gctggagatc ggcgacgctg tcgaactgga tgccgacgcc cgcggcaagc
     5881 gcgagcgcgg tgagaccgcc gtccgcgttg tcgccgaagc cacggaatcc
//
LOCUS       DAOSIF010000142         5887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12232_length_5887_cov_3.351166, whole genome shotgun sequence.
ACCESSION   DAOSIF010000142 DAOSIF010000000
VERSION     DAOSIF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12232_length_5887_cov_3.351166"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..549)
                     /locus_tag="PLT35_06205"
     CDS             complement(142..549)
                     /locus_tag="PLT35_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28858.1"
                     /translation="MALGLVWDLAFPINKKIWTSSYVWFTGGAAAVFLAMCFALIDVR
                     GWKAWSRPFVILGVNAIALYTLSGLLSKFLSSHRIVLADGSALAWSRYLYTTYFAPLA
                     SPINASLLWAAAHLAILFVVLWVMDKRGIYLKA"
     gene            complement(697..1275)
                     /locus_tag="PLT35_06210"
     CDS             complement(697..1275)
                     /locus_tag="PLT35_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOG28859.1"
                     /translation="MSKPARLTSLDAFRGLTMAGMVIVNNPGDWNNVYAPLLHAEWNG
                     CTPTDLIFPFFLFIVGVSITLSRGTMANPWKIVKRAVVIFGLGLILALYPRWDFSVVR
                     IPGVLQRIALCYLAAAFLFRWTAPAHGSDDARRWSQGRTLLIVAAALTLAYWALMVLV
                     PVPGVAAGQHLTPGNDLGAYIDRHVFGAHLYS"
     gene            1392..2438
                     /gene="amrS"
                     /locus_tag="PLT35_06215"
     CDS             1392..2438
                     /gene="amrS"
                     /locus_tag="PLT35_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOG28860.1"
                     /translation="MAALTREGASALYDAPGGGWVQCHACAHECEIAEGAPGTCRVRF
                     NRGGVLRVPWGYVAGAQSDPIEKKPFFHVEPGCRAFSFGMLGCDFKCACCQNWVTSQA
                     LRDPAAGASLVETTPEMLVHAAIRSGARAVVSTYNEPLITTEWAVDIFKAARASGLLT
                     GYVSNGHATPRVLDYLAPWIDVFKVDLKGFDELQYRNYGGRLEPVLDTIRRLHAMGIW
                     VEVVTLLVPGLNNSDDEIGRVASFLAGVSPDIPWHVTAFHPDYRMTDRAATTPAMLVR
                     AAGIAARAGLRFVYAGNAAGEVGDLEDTRCPSCRQILVERRAYWIGVCRVTADGRCPA
                     CGTVIPGRWRTAAP"
     gene            2435..3466
                     /gene="moaA"
                     /locus_tag="PLT35_06220"
     CDS             2435..3466
                     /gene="moaA"
                     /locus_tag="PLT35_06220"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOG28861.1"
                     /translation="MTGGAPLIDTLGRALRTLRLSVTDRCNLRCHYCMPEPEYAWLPR
                     DLLLRFEEATRLVRVLTGLGVRAVRLTGGEPLLRHGLPDLVASLAAQPLADLALTTNG
                     LLLERLAEPLKRAGLKRLTVSLDTLAADRFRALTGQGGLSRVVGGIRAAARCFGGVKI
                     DAVILRGVNDDELADLLEFGAEVGAEVRFIEYMDVAGATRWTADAVVPSSEMIGRVRA
                     RYGSAEPIEEPDSAAPATRFALPDGRTFGIVASTTSPFCRACDRVRLTADGTLYMCLY
                     GTEGLALRDPMRSGATDEALAGLVAARWRARADRGAEERASLPVRRAYLPLRTLRADP
                     RLEMHSRGG"
     gene            complement(3496..4584)
                     /locus_tag="PLT35_06225"
     CDS             complement(3496..4584)
                     /locus_tag="PLT35_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HOG28862.1"
                     /translation="MDSVRGRVAGGIAVAAAAAAALAPLPPAWIESHYSRGFYPAFQR
                     ALTRASNAVPIAAFDVLVVAAACAVPLGVVVAMRAPKGGRLRLAGRLAWRGVVAAALV
                     YLAFLVAWGLNYRREPVESRLDFDERRVTAEAVVGLNDAAVAELGRLRPLLPEQYAEW
                     PTAAAVARALAGALEQGSRLLALPAPITPGRPKRSILDPFFTRAGVSGFTDPFFLETL
                     LPSNLLPFELPSVVAHEWGHLAGLARESEASLFAAVVCLHGDETAQYSAWLEVFAGTL
                     IARDAEGRREARGRLPAAVRADLDAMAARSARDEVRLVRRAAWRAYDRYLRSQRVASG
                     AADYGGVVRLLAGLRFESGQPAAARPER"
     gene            4670..>5887
                     /gene="hypF"
                     /locus_tag="PLT35_06230"
     CDS             4670..>5887
                     /gene="hypF"
                     /locus_tag="PLT35_06230"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOG28863.1"
                     /translation="MPVQRSPAPDEGRRLEIRGIVQGVGFRPWVYRLAREHGLGGWVC
                     NDAAGVAIEVCGPAASIEAFESALRSTPPPASRIVSVEARRVEASWPAGAFEIRASAP
                     AADRRVSIPPDLATCPDCLAEVLDPRDRRAGYPFTNCTHCGPRFTIAFDVPYDRRNTT
                     MAAFRMCPACQAEYDSPGDRRFHAQPNACPACGPRVWLTDDEGRERPSRDAIGEAASA
                     LLSGRIVAVKGLGGFHLACDASDEQAVARLRARKRRDEKPFAVMAATLGDAELLADLG
                     DPERDLLTSIERPIVLAPRRRGAPLAANVAPGNSMVGVMLPYTPLHHLLMKAVGRPLV
                     MTSGNLSEEPIACRNDEALVRLCAIADLFLLHDRDIDTRCDDSVARVIAGAPVVLRRS
                     RGYVPKGIPAAGRF"
BASE COUNT          863 a         2187 c         2063 g          774 t
ORIGIN      
        1 gaccctcgtc aggtagagcg gtatgatcgg gtagacggcc tccgaggcta cgtcggtgaa
       61 gaagctcgtc cacgagacga gccacaccgg tctgggcagc cgcggccgcg cgcctgtcgg
      121 cttcgccggg cgggccatgc gtcaggcctt gaggtagatg ccgcgcttgt ccatcaccca
      181 gagcacgacg aacaggatcg ccaggtgcgc cgcggcccac agcagcgacg cgttgatcgg
      241 gctggcgagc ggcgcgaagt acgtcgtgta gaggtagcgg ctccaggcga gggcgctgcc
      301 gtcggcaaga acgatccggt ggctcgacag gaacttgctg agcaggccgg acagggtgta
      361 gagggcgatg gcattcacgc cgaggatgac gaacggccgg ctccacgcct tccaaccgcg
      421 cacgtcgatg agcgcgaagc acatcgccag gaacaccgcc gccgccccgc cggtgaacca
      481 gacgtaggag ctcgtccaga tcttcttgtt gatgggaaac gccaggtccc acacgagccc
      541 cagcgccatg gcggcaacgc cggcgccgag cagccacgcc gccttccgcc tgccggttgc
      601 ccgcgagccc agccagaggc cggccgcgcc gccgaggagc gtcgtggcaa tggaggggag
      661 cgtgctcagc aggccctcgg gatcccacgt cttcgtctac gagtagaggt gcgcgccgaa
      721 cacgtggcga tcgatgtagg cgccgaggtc gttgcccggc gtcaggtgct ggcccgccgc
      781 gacgcccgga accgggacga gcaccatcag cgcccagtag gcgagggtca gcgcagccgc
      841 cacgatcaac agggtccggc cctgcgacca gcgcctggcg tcgtccgacc cgtgcgcagg
      901 cgcggtccag cggaacagga acgccgcggc gaggtagcag agggcgatcc gctgcagcac
      961 gccggggatg cgcacgacgc tgaaatccca tcgcgggtag agcgccagga tcaggccgag
     1021 gccgaagatc acgacggcgc gcttcacgat cttccagggg ttcgccatcg tgccgcgcga
     1081 caaggtgatg gacacgccga cgatgaagag gaagaacggg aagatcaggt ccgtcggcgt
     1141 gcagccgttc cattcggcgt gcaggagcgg cgcgtacacg ttgttccagt cgccggggtt
     1201 gttcacgatc accatcccgg ccatggtcag gccgcgaaag gcgtcgagcg acgtcaggcg
     1261 ggcgggcttc gacacgcggg acctcctgct gctcgcgcca tcatgccccg atccgaccgc
     1321 cggggaaagg gcctgttgca aatggcggac gccggcgcta caactggagc gtgacgagcc
     1381 tcggagaccg catggccgcc ctcacccggg agggcgcctc cgcgctgtac gacgcgccgg
     1441 ggggcggctg ggtgcagtgc cacgcctgcg cccacgagtg cgagatcgcc gagggcgccc
     1501 caggcacctg ccgcgtgcga ttcaaccggg gcggcgtcct gcgcgtgccc tggggctacg
     1561 tcgccggcgc ccagagcgac cctatcgaga agaagccctt ctttcacgtc gagcccggct
     1621 gccgcgcctt cagcttcggg atgctgggct gcgatttcaa gtgcgcgtgc tgccagaact
     1681 gggtcacctc gcaggcgctc agggacccgg ccgcgggcgc cagcctcgtc gaaacgaccc
     1741 ccgagatgct cgtgcacgcc gccatccgct ccggcgcgcg ggccgtcgtc agcacctaca
     1801 acgagccgct catcaccacc gagtgggcgg tggacatctt caaggccgcc cgcgcgtcgg
     1861 ggctgctcac cggttacgtc tccaacggtc acgccacgcc cagggtcctg gactacctgg
     1921 cgccctggat cgacgtcttt aaagtcgacc tgaaaggctt cgacgaactg cagtaccgga
     1981 actatggagg ccgcctcgag ccggtcctcg acacgatccg ccgactccac gcgatgggca
     2041 tctgggtcga ggtcgtgacg ctcctcgtgc ccggcctcaa caactccgac gacgagatcg
     2101 gccgagtcgc ctcgttcctc gccggcgtgt cgcccgacat cccgtggcac gtgacggcct
     2161 tccatcccga ctaccgcatg accgaccggg ccgccacgac gccggccatg ctcgtgcgcg
     2221 cggcgggcat cgccgcgcgc gcggggctcc gcttcgtcta cgccggcaac gcggcgggcg
     2281 aagtcggcga cctggaagac acccgctgtc cctcgtgccg gcagatcctc gtcgaacggc
     2341 gggcgtactg gatcggcgtg tgccgcgtca ccgccgacgg gcgctgcccc gcctgcggga
     2401 ccgtcattcc gggccgctgg cggacggccg cgccatgact ggcggcgccc cgctcatcga
     2461 cacgctcggc cgggcgctga ggacgcttcg gctgtcggtc accgaccgct gcaatctgcg
     2521 ctgccactac tgcatgccgg agccggagta cgcctggctg ccccgcgacc tcctcctgcg
     2581 cttcgaggaa gcgacaaggc tcgtccgcgt gctcaccggg ctcggcgtta gggcggttcg
     2641 cctgaccgga ggcgagcccc tgctccgcca cggacttccc gacctcgtgg cgtcactagc
     2701 cgcgcagccg ctcgccgacc tggcgttgac gaccaacggc cttctcctcg agcgcctggc
     2761 cgagccgctg aagcgggccg gcctgaaacg cctcaccgtc agcctggaca cgctcgcggc
     2821 ggaccggttc cgcgcgctca cggggcaggg cgggctctcc cgcgtcgtgg gcggaatccg
     2881 cgccgcggcg cgctgcttcg gtggcgtcaa gatcgacgcc gtcatcctgc gcggcgtcaa
     2941 cgacgacgaa ctcgcggacc tcctcgaatt cggcgcggag gtcggcgcgg aggtccggtt
     3001 catcgagtac atggatgttg cgggcgcgac gcgatggacg gcggacgcgg tggtgccctc
     3061 ctccgagatg attgggcgcg tccgcgcgcg ctatggatcc gcggagccga tcgaagagcc
     3121 ggattcggct gcgccggcca cgcgcttcgc gcttcccgac ggccggacgt tcgggatcgt
     3181 cgcctccacg acgtccccgt tctgccgggc ctgcgaccgc gtccgcctca cggccgacgg
     3241 cacgctgtac atgtgcctct acggcaccga gggcctcgcc ctgcgcgatc ccatgcggag
     3301 cggcgcgacc gacgaggcgc tcgcgggcct cgtcgccgca cgctggcgcg cccgcgccga
     3361 ccgcggggcc gaagagcgcg cgagcctgcc ggtgcggcgg gcctacctgc ccctcaggac
     3421 gctgcgcgcc gaccctcgcc tggagatgca cagccgcggc gggtagcgtc gggcagccgc
     3481 tggcagcggg ggtgttcagc gctccggccg ggccgcggcc ggctggcccg actcgaaccg
     3541 cagcccggcg agcagccgca cgaccccgcc gtagtcggcc gcgcccgacg ccacacgctg
     3601 cgatcgcagg taacggtcgt aggcgcgcca cgccgcgcgc cggaccaggc gcacctcgtc
     3661 gcgggcgctg cgcgccgcca tcgcgtcgag atcggcgcgc accgcggcgg gcaggcggcc
     3721 gcgcgcctcg cgcctcccct ccgcgtcacg cgcgatcagc gtgcccgcga agacctcgag
     3781 ccacgcgctg tattgcgcgg tctcatcgcc gtgcagacag acgacggccg cgaacagcga
     3841 cgcctccgat tcgcgcgcca ggcccgcaag gtgcccccac tcgtgcgcga cgaccgacgg
     3901 cagctcgaac ggcaggaggt tcgacggcag gagcgtctcg aggaagaacg ggtcggtgaa
     3961 gccgctgacg ccggcgcggg tgaagaacgg gtccaggatc gagcgcttcg gcctgccggg
     4021 cgtgatcgga gcgggcaggg ccagcaggcg gctcccctgc tcaagggccc cggccagagc
     4081 ccgagccacg gcggccgccg tcggccactc ggcgtactgc tcgggaagca gcggccggag
     4141 ccggccgagt tcggcgacag cggcgtcgtt cagccccacg accgcctccg ccgtcaccct
     4201 gcgctcgtcg aaatcgaggc gcgactcgac aggctcgcgc cggtagttga gcccccaggc
     4261 caccaggaac gccaggtaga cgagtgcagc ggccaccaca ccccgccacg ccagccgacc
     4321 ggccagccgc agccgtccgc ccttcggcgc ccgcatcgcg accacgactc cgagcggcac
     4381 cgcgcaggcg gcagccacga cgagcacgtc gaaggcggct atcggcacgg cgttcgacgc
     4441 gcgcgtcagg gcgcgctgga aggccggata gaacccacgc gagtagtggc tctcgatcca
     4501 cgccggcggc agcggcgcca gcgccgccgc cgcggctgcg gcaacggcga tccctccggc
     4561 cacccttccg cgcacgctgt ccatgatagc cggatgcccg cgtcctcggc cgcccgccgg
     4621 ccgggccgcc gctggactcg cgctgggcaa acagctataa acgaacgcgg tgcctgtcca
     4681 gcgcagcccc gctccagacg aaggccgccg cctcgagatc cgcggaatcg tccagggcgt
     4741 agggttccgc ccgtgggtgt accgcctggc ccgcgaacac ggcctcgggg gctgggtctg
     4801 caacgacgcc gccggcgtcg ccatcgaggt gtgcggcccg gccgcctcga tagaggcgtt
     4861 cgaatccgct ctccgttcga cgccccctcc ggcctcccgg atcgtatcgg tcgaggcccg
     4921 ccgggtcgaa gcgagctggc cggccggcgc gttcgagatc cgcgcgtcgg cgccggcggc
     4981 ggaccgccgg gtgtcgatcc cgcccgatct ggccacctgt cccgattgtc tcgccgaagt
     5041 cctcgacccg cgagatcgac gcgcgggcta tccgttcacg aactgcacgc actgcggccc
     5101 gcgcttcacc attgccttcg acgtccccta cgaccggcgc aacacgacca tggcggcgtt
     5161 ccggatgtgc ccggcctgcc aggccgaata cgactcgccg ggcgaccggc ggttccacgc
     5221 ccagcccaat gcctgccccg cgtgcggccc gcgcgtgtgg ctgacggatg acgaagggcg
     5281 cgagcggcct tcgcgcgacg ccatcggcga ggcggcctcg gccctgctca gtggccggat
     5341 cgtggccgtc aaggggctgg gcggctttca cctcgcgtgc gacgcgtcgg acgagcaggc
     5401 ggtcgcgcgg ctgagggcgc gaaaacgccg cgacgaaaag ccgttcgcgg tcatggccgc
     5461 gacgctcgga gacgcggagc tgctggcgga cctgggcgac cccgaacgcg acctgctgac
     5521 ctccatcgag cgccccatcg tcctggctcc ccggcgccgg ggggcgccgc ttgcggcgaa
     5581 cgtcgcgccg ggcaactcga tggtcggcgt gatgctgcct tacacgccgc tgcaccatct
     5641 gctcatgaag gctgtcggcc ggccgctcgt gatgacctcg ggcaacctgt ccgaggagcc
     5701 gatcgcctgc cggaacgacg aggcgctcgt ccgcctctgc gccatcgccg acctcttcct
     5761 gctgcacgac cgcgacatcg acacccgctg cgacgactcg gtggcgcgcg tgatcgctgg
     5821 cgcgcccgtc gtgctccggc gctcgcgcgg ctacgtcccg aagggaatcc cggcggccgg
     5881 ccgcttc
//
LOCUS       DAOSIF010000143         5884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12239_length_5884_cov_4.906330, whole genome shotgun sequence.
ACCESSION   DAOSIF010000143 DAOSIF010000000
VERSION     DAOSIF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12239_length_5884_cov_4.906330"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLT35_06235"
     CDS             complement(<1..188)
                     /locus_tag="PLT35_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOG28864.1"
                     /translation="MGLAHLATRFTCSWPWSTLVLLCDGRVVCGCADPYAARVVGDAR
                     TSTLHEIWTGAPMAALRAG"
     gene            complement(208..477)
                     /locus_tag="PLT35_06240"
     CDS             complement(208..477)
                     /locus_tag="PLT35_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28865.1"
                     /translation="MRTIKTTTGADIPLDGDLLTVMETLFQEVTARRELERSFDDMVR
                     EIQHLVGQMTEAEREAYLRESLFLNTVTYENERLGAYMRRLGQGA"
     gene            complement(480..821)
                     /locus_tag="PLT35_06245"
     CDS             complement(480..821)
                     /locus_tag="PLT35_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG28866.1"
                     /translation="MTLTIDHVRGVAVVRVAESRLMYPLLSDFAAAVGGLVAAGTPKV
                     IIDLSPVVYIDSATIGCLMDLYRQAAAAGGSLKLAGVQRRVETMLTMTGAHNFIQLFA
                     DEAAALASFEG"
     gene            complement(824..1453)
                     /locus_tag="PLT35_06250"
     CDS             complement(824..1453)
                     /locus_tag="PLT35_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG28867.1"
                     /translation="MPPEPTAPTTDVNALIERCLQGDQQAWDAIVRLHWRRVFNVAYK
                     FVGRHDEAEDLTQDVFLKVYRSLGTFDRRANFQTWLISVSRNLCIDHYRSVRKERETI
                     DRDVNADDLSPVSREVNPLVALERRDLARELRRALDELPRALRAAVVMRDLQDLSYQE
                     IADRLGLPEGTVKSRINRGRHELARQITRLREEDQARGAAPRQAGVSGA"
     gene            1537..2778
                     /gene="lysA"
                     /locus_tag="PLT35_06255"
     CDS             1537..2778
                     /gene="lysA"
                     /locus_tag="PLT35_06255"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOG28868.1"
                     /translation="MTGFGFRGGRLACEGVPVADIARAAETPVYVYSAGSVRAAYASL
                     DRALAAWPHAIHFALKANSALALLALLRELGAGADANSGGEIEAALRAGFSPPQIVFT
                     GVGKTRDELERAVALGVGAINIESGGEAERIDAIARGRGVRARVALRANPDIAPNTHA
                     HISTGSRLSKFGVPIECAAGLLREMARMPGLEPVGLHVHLGSQILDVAPLGRAAGLLA
                     GLARAARDWGLALEHIDLGGGLGVPYEGGAGPSVDEYAAAILPAVRETGLRLIVEPGR
                     YLVAQAGALVGRVADTKAYPGSPRFVILDAGMTELIRPMLYEAYHRIVEVEPRGGAAT
                     PCEVVGPLCETGDTLGHARELGPVEVGDLVAVLDAGAYGFVMASNYNRRRLPAEVLVD
                     GERWQIVRKRQSYEDLFRHEV"
     gene            2862..3455
                     /gene="tmk"
                     /locus_tag="PLT35_06260"
     CDS             2862..3455
                     /gene="tmk"
                     /locus_tag="PLT35_06260"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOG28869.1"
                     /translation="MSGLLIVFEGLDQSGKETQARLLRRWVEARGREVESLAFPDYDT
                     PIGAEIWKGLHQERDFGPDTLQLLLIANRFERKQAIQGWLAAGRVVICDRYLASSVAY
                     GEAQGLDPRWLTAAQVCLPQPDLTVLLDIAPEVAAARKATGRDKFERDLSMLARVRAS
                     YLRQAADLLWVVIDAARGVEAVADEVATAVARRLGLP"
     gene            complement(3421..4212)
                     /locus_tag="PLT35_06265"
     CDS             complement(3421..4212)
                     /locus_tag="PLT35_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOG28870.1"
                     /translation="MRGAAGRAAGLAREALSALASVVIAPECAACGRALERPDLGAVC
                     PACWQAVRRFTPPVCPACGISVSPHATGPAGPCEACQSGARVVSAARAVGPYEGRLRE
                     IIHACKYERRPSLAGPLATLLLEAGREVLDGADVVVPVPLHWTRRHQRGFNQAEALAT
                     RLGVPVARALRRTRRTRPQVELPAGDRHRNVSGAFGLARCAGGPPGRVRWRRGARPLA
                     GRIVVLVDDVATTGATLDACARVLLEAGAREVRALTAARVASPRR"
     gene            complement(4262..4717)
                     /gene="rsfS"
                     /locus_tag="PLT35_06270"
     CDS             complement(4262..4717)
                     /gene="rsfS"
                     /locus_tag="PLT35_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG28871.1"
                     /translation="MSRTPSARAKTAGGPGPGPRTRKAGGKAAADTAGHEPPIALVRA
                     ARAAAEKKATDVVALDLRQVQAFTDFFLICTGQNTRQVRAIADAVEAALGKHGEKPAH
                     VEGYDRANWILLDYFSFVVHVFAPEARAFYDIERLWGSASRLSLPDAAG"
     gene            complement(4710..5468)
                     /gene="nadD"
                     /locus_tag="PLT35_06275"
     CDS             complement(4710..5468)
                     /gene="nadD"
                     /locus_tag="PLT35_06275"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOG28872.1"
                     /translation="MPSRTRRLGVLGGTFDPIHLGHLAAATAAASALGLEEVLLVPSR
                     TPPHRRVDPAASVFHRFAMVALAAATDPRLVASDLELDRTGASYTADTLRALHATGLA
                     AWQIFFTTGADAFAEIATWREYPSVLELAHFVVCARAGIEASSLRARLPELAGRMVLL
                     APNVWDAGRSAVGRPRQEGDEPWNGRETRIFLLDGQTPDISSTAVRARARAGRPLAGL
                     VPPEVDDYIRRHGLYGAPAGAADGGRSAAGNLHE"
     gene            complement(5461..>5884)
                     /locus_tag="PLT35_06280"
     CDS             complement(5461..>5884)
                     /locus_tag="PLT35_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOG28873.1"
                     /translation="LGVVALDDDRSFVVADVPGLIEGAHRGLGLGHQFLQHLERTRVL
                     VHVVDVSGASGRDVVEDFDTIRRELRLYRPELADKPAIAAANKMDAAADAGEADRLDA
                     HVAPLGIPIHRISAVTGRGVAALVEDMWRHLQGSVPDA"
BASE COUNT          806 a         2036 c         2222 g          820 t
ORIGIN      
        1 ccggcccgca gcgcggccat cggcgccccg gtccagatct cgtgcagcgt ggaggtccgc
       61 gcatcgccca cgacgcgcgc ggcgtacgga tcggcgcagc cgcacaccac ccggccgtcg
      121 cagagcagaa ccagtgtgct ccacggccag gagcacgtga accgcgtggc gaggtgggcg
      181 agccccatcg gcagacgcgc cgtccgccta cgcgccctgg cccagcctgc gcatgtaggc
      241 gccgaggcgt tcgttctcgt aggtgacggt gttcaggaac aggctctccc gcaggtacgc
      301 ttcccgctcc gcctcggtca tctggccgac gaggtgctgg atctcccgca ccatgtcgtc
      361 gaacgaccgc tcgagctccc gccgcgccgt cacttcctgg aagagggtct ccatgacggt
      421 cagcaggtcg ccgtcgaggg ggatgtccgc gccggtggtc gtcttgatcg tccgcatggc
      481 tacccctcga agctggcgag cgcggccgcc tcgtcggcga acaactggat gaagttgtgc
      541 gcgccggtca tcgtcagcat cgtctccacc cgccgctgca caccagcgag cttcagcgac
      601 ccgccggccg ccgcggcctg gcggtagaga tccatcaggc agccgatggt ggcgctgtcg
      661 atgtagacga cgggcgagag atcgatgatc accttggggg tgccggcggc cacgaggccg
      721 cccaccgccg ccgcgaaatc ggagagcagc gggtacatca gccggctctc ggcgacgcgg
      781 acgacggcga cgccccgaac gtggtcgatg gtcagcgtca tgctcatgct ccggacaccc
      841 cggcctggcg gggcgcggcc cctctggcct ggtcctcttc tctgagacgc gtgatctggc
      901 gcgcgagttc gtgccggccg cggttgattc gggacttcac cgtgccttca ggcaggccca
      961 gccggtcggc gatctcctgg taggagagat cctggaggtc ccgcatcacg accgccgcgc
     1021 gcagcgccct tggcagctcg tcgagggcgc ggcgcagctc ccgtgcgagg tcgcggcgct
     1081 cgagggccac gagcgggttg acttccctcg agaccggcga caagtcgtcg gcgttcacgt
     1141 cccggtcgat cgtctcccgc tccttgcgga cgctgcggta gtgatcgatg cacaggttgc
     1201 ggctcacgct gataagccag gtctggaagt tggcgcggcg gtcgaacgtg ccgagcgacc
     1261 ggtagacctt gaggaacacg tcctgcgtca agtcctcggc ttcgtcgtgc cggcctacga
     1321 acttgtaggc gacgttgaac acccgccgcc agtggaggcg gacgatggcg tcccaggcct
     1381 gctgatcgcc ctggaggcag cgctcgatga gcgcgttgac gtccgtcgtc ggggcggtcg
     1441 gctctggcgg catgtcgtca gcgagacagc gccgcagaat accgcgtgag ccggggggtg
     1501 tcaaacgcgg aggaggtcgg gtaagatgcc cggtctgtga ccggatttgg cttccgcggc
     1561 gggcggctcg cgtgcgaggg cgtcccggtg gccgacatcg cgcgcgcggc ggagaccccc
     1621 gtgtacgtct acagcgccgg gagcgtgcgg gccgcgtacg cgagcctcga ccgggcgctc
     1681 gccgcctggc cgcacgccat ccacttcgcc ctgaaggcca actcggcgct ggcgctcctg
     1741 gccttgctgc gcgaactggg ggccggggcc gacgcgaact cgggcgggga gatcgaggcg
     1801 gcgctgcgtg cgggcttctc gccgccgcag atcgtcttca ccggcgtcgg gaagacgcgg
     1861 gacgaactcg agcgggccgt cgcgcttgga gtcggcgcca tcaacatcga gtcgggcggg
     1921 gaggcggagc gcatcgatgc catcgcccgc gggcggggcg tgcgggcgcg agtggcgctg
     1981 cgcgccaatc cggacatcgc accgaacact cacgcgcaca tctcgaccgg ctcgcggctc
     2041 agcaagttcg gcgttcccat cgagtgcgcc gccggcctcc tgcgggagat ggcgcgcatg
     2101 ccgggactcg agccggtggg cctgcacgtc cacctcggct cccagatcct cgacgtcgcg
     2161 ccgctcgggc gggcggccgg cctcctcgcc ggcttggccc gggcagccag ggactggggc
     2221 cttgccctcg agcacatcga ccttggcggc ggcctgggcg tgccctacga gggcggagcg
     2281 ggtcccagcg tcgacgagta cgccgcggcc atcctgcccg ccgtccggga gacgggcctc
     2341 cgcctgatcg tcgagccggg ccggtacctt gtcgcacagg cgggcgccct ggtgggccgc
     2401 gtcgccgaca cgaaggcgta ccccgggtct ccgagattcg tcatcctcga tgccggcatg
     2461 acggaactca tcaggcccat gctgtacgag gcgtaccatc gaatcgtcga ggtagagccg
     2521 cgcggcggag cggcgacgcc gtgcgaggtc gtgggtccgc tgtgcgaaac aggcgacacg
     2581 ctcgggcacg cccgggaact ggggccggtc gaggtgggcg acctcgtggc cgtgctggac
     2641 gcgggggcgt acggattcgt tatggcgtcc aactacaacc ggcggcggct gcccgcggaa
     2701 gtgctcgtgg acggtgagcg ctggcagatc gtccgcaaac ggcaatcgta cgaggacttg
     2761 ttcaggcacg aggtctgagc agggccacgg gaccaggggc gaggattcgg cgctccgaat
     2821 gccggttcgg ggttctggct tccggcgtcc ggcgggttct catgagcgga ctgctgatcg
     2881 tcttcgaggg cctcgatcag agcggcaagg aaacgcaggc gcgcctgctg cgccgatggg
     2941 tcgaagcgcg cggccgggag gtcgagtccc tggcgtttcc ggattacgac acgccgatcg
     3001 gggccgagat ctggaagggc ctgcatcagg agcgggactt cggccccgac acgctgcaac
     3061 tgctgctcat cgcgaaccgt ttcgagcgga agcaggcgat ccagggatgg cttgccgcgg
     3121 gccgggtcgt gatttgcgac aggtacctcg cgtcgagcgt ggcctacgga gaggcgcagg
     3181 gcctcgaccc gcgctggctg accgcagccc aggtctgcct cccgcagccc gacctgacgg
     3241 tgctgctcga catcgcgccc gaggtggccg cggcgcggaa ggccaccggg cgggacaagt
     3301 tcgagcgcga tctctccatg ctggctcggg tgcgggccag ttacctgcgg caggcagcgg
     3361 atctcctgtg ggtcgtcatc gacgcggcgc gcggggtcga ggccgtcgcc gacgaggtcg
     3421 ctaccgccgt ggcgaggcga ctcgggctgc cgtgagcgcc cgcacctccc gagcgcccgc
     3481 ctccaggagg acgcgagcgc aggcgtcgag tgtggcgccg gtcgtcgcca cgtcgtcgac
     3541 gaggacgacg atgcggccgg cgagcggccg cgcgccgcgg cgccagcgaa ctcggcccgg
     3601 cggcccgccg gcgcaccggg cgaggccgaa cgccccgctg acgttgcggt ggcggtcgcc
     3661 ggccggcaac tcgacctgcg gcctggtgcg cctggtcctt ctgagcgccc gggcgacggg
     3721 aacgccgagg cgggtcgcga gggcttcggc ctggttgaat cccctctgat ggcggcgtgt
     3781 ccagtggagc ggcaccggca ccacgacatc ggccccgtcc aggacctcgc gtcccgcctc
     3841 gaggagcagc gtcgcgagcg ggccggcgag cgaggggcgc cgctcgtact tgcaggcgtg
     3901 aatgatctcg cgcaggcggc cttcataggg gccgacggca cgcgcggccg agacgacgcg
     3961 cgcgcccgat tggcaggcct cgcacgggcc ggctggcccg gtcgcgtgcg gcgacacgga
     4021 tatcccgcac gcggggcaga ccgggggagt gaagcggcgg acggcctgcc agcaggcggg
     4081 gcagacggcc ccgaggtcgg gccgctcgag tgcgcgtccg cacgccgcgc actcgggcgc
     4141 aatgacgacg gacgccaggg cgctgagggc ctcgcgggcg agccccgccg cgcgccccgc
     4201 ggcgccgcgc aaggcgacga agccgacgcg gatggccgtc ctccgcgcca gccgggcggc
     4261 ctcagcccgc ggcgtccggc aggctgagcc ggctggcgct gccccagagc cgctcgatgt
     4321 cgtagaaggc ccgcgcctcc ggcgcgaaga cgtggacgac gaagctgaag tagtcgagca
     4381 ggatccagtt cgcccggtca tagccttcga cgtgggcggg cttctcgccg tgctttccca
     4441 gcgccgcctc gaccgcgtcc gcaatcgccc gcacctgccg ggtgttctgg cccgtgcaga
     4501 tcaggaagaa gtccgtgaac gcctgcacct ggcgcaagtc gagcgccacg acgtcggtgg
     4561 ccttcttttc ggctgcggcc cgcgccgccc ggacgagtgc gatcggcggt tcgtgccccg
     4621 ccgtgtcggc ggccgctttg ccgccggcct ttcgggttcg cgggcccggg ccgggtccgc
     4681 ctgcggtctt tgcgcgcgcg ctcggagttc tactcatgca agttgccggc cgcggacctg
     4741 ccaccgtcgg cggcgccagc cggagcgccg tagagaccgt ggcggcggat gtagtcgtcg
     4801 acttcgggag gcaccaggcc cgccaggggc cgccctgcac gcgcacgcgc gcgaaccgcc
     4861 gtggatgaga tgtcgggcgt ctggccgtca aggaggaaga tgcgcgtttc ccgcccgttc
     4921 cagggctcgt cgccttcctg gcgagggcgg ccgacggcgc tgcggccggc gtcccagacg
     4981 ttcggagcga gcaggaccat ccgtccggcc agctccggca gccgggcccg aagcgaggag
     5041 gcctcgatgc cggcacgcgc gcagacgacg aagtgagcga gctccagcac gctcggatac
     5101 tcccgccagg tggcaatttc cgcgaaggca tcggccccgg tcgtgaagaa aatctgccac
     5161 gcggcgagcc ccgtcgcgtg gagcgcgcgg agcgtgtcgg ccgtgtacga cgcgccggtg
     5221 cgatcgagtt cgaggtcgct ggcgaccagc cgcggatcgg tcgctgccgc cagcgcgacc
     5281 atcgcgaagc ggtggaagac cgacgcggcc ggatcgacgc ggcggtgggg cggcgtgcgc
     5341 gacgggacca gcaggacctc ctcgagcccc agtgcggagg cggctgccgt cgcggcggcg
     5401 aggtggccga ggtgaatggg gtcgaaggtg ccgccgagga cgccgagccg ccgcgtccgg
     5461 ctaggcatcc ggcaccgagc cttgcaggtg acgccacatg tcctcgacga gggcggccac
     5521 gccgcggccg gtgaccgccg agatgcggtg gatcgggatt ccgagcggcg cgacgtgcgc
     5581 gtcgaggcgg tcggcctctc ccgcgtcggc tgccgcgtcc atcttgttcg cggcggcgat
     5641 ggccggcttg tcggcgagct cggggcggta gaggcggagc tcgcgccgga tcgtgtcgaa
     5701 gtcctcgacg acgtcccgcc cggacgcgcc cgacacgtcc acgacgtgca ccagcacgcg
     5761 ggtgcgctcg aggtgctgga ggaactggtg gccgaggccg aggccgcggt gcgcgccctc
     5821 gatgagtccc ggcacgtccg cgacgacgaa gctgcggtcg tcgtcgagcg cgacgacgcc
     5881 gagg
//
LOCUS       DAOSIF010000144         5883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12244_length_5883_cov_3.959677, whole genome shotgun sequence.
ACCESSION   DAOSIF010000144 DAOSIF010000000
VERSION     DAOSIF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12244_length_5883_cov_3.959677"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLT35_06285"
     CDS             complement(<1..347)
                     /locus_tag="PLT35_06285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOG28874.1"
                     /translation="MTEVLSGCPFCNPAPGAIIAGNALAVALSDAYPVSPGHALIVPR
                     RHIASWFEVSAAEREALMALVDEVKERLDHDRRPDGYNIGMNVGAAAGQTVMHFHLHL
                     IPRFAGDVDDPTGG"
     gene            complement(524..664)
                     /locus_tag="PLT35_06290"
     CDS             complement(524..664)
                     /locus_tag="PLT35_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28875.1"
                     /translation="MQPTAQYDWIDVDNHFPYRIYGGQQDNSTVATASRELRGARLPR
                     KE"
     gene            783..>1304
                     /locus_tag="PLT35_06295"
     CDS             783..>1304
                     /locus_tag="PLT35_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28876.1"
                     /translation="MHRRVLSVLVLTPILAAVLHSSMTAMTPQAAAAGAARENPLMKP
                     SPLPFGAPAFDKIQDADFKPAMEAGIKAELEEVTAIAANAAAPTFENTLVALERRGVL
                     LRRVSLILNALASANTNPTIQALQEELAPKLAALDDAIFLNEKLFARVETVYESRASL
                     TLDPESARLLEFYY"
     assembly_gap    1305..1314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1315..2935
                     /locus_tag="PLT35_06300"
     CDS             <1315..2935
                     /locus_tag="PLT35_06300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOG28877.1"
                     /translation="GAADKAALKALNEQEAGLSAKFVNQLLAATKAGALVVSDPAELA
                     GLTKDELEAAAIAAKSRGLDGKWVLPLRNTTQQGPLASLTNRATREKLFRASWTRAER
                     GDANDTRQTIATLASLRARKAALLGYPDFAAWVLEDQMAKTPGAVNAFLGRLIPPATA
                     NARAEAKDIQAIIDSQKGGFQLAPWDWDLYAEQVRKAKFDLDSATVSQYLELDRVLRD
                     GVFFAAQELYGLSFKERRDLPVYQQDVRVFDVIDRDGSPLALFYFDPYARDNKNGGAW
                     MDNFVVQSKLLGTKPVVFNCANIAKPAPGQPVLLAFDEVLTLFHEFGHALHGIFADQQ
                     YPSLSGTSVARDFVELPSQFNEHWALDAKVLSRYARHHKTGEPMPQALADKLKAAATF
                     NQGYSLTEILGAAVLDMRWHSMPAGSGEQPAGPFEAGVLEQSGLNLPQVPPRYRSSYF
                     LHIWANGYEAGYYAYLWAEMLDNDAYQWFVEHGGLTRANGDRFRQMILSRGNAGDYAK
                     LYREFRGQDPSIEPMLKRRGISTAAAPAKRP"
     gene            3118..3279
                     /locus_tag="PLT35_06305"
     CDS             3118..3279
                     /locus_tag="PLT35_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28878.1"
                     /translation="MVAAATEGLELLDDEADCARQINMVMASAEEMTKDLVKRYGGTT
                     RKRAGRKKA"
     gene            3439..4623
                     /locus_tag="PLT35_06310"
     CDS             3439..4623
                     /locus_tag="PLT35_06310"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOG28879.1"
                     /translation="MRSVKSLYKIGHGPSSSHTMAPRFAAEAFLRRHPAATRVTVTLY
                     GSLAATGKGHLTDIALREALGKTAVELRWEPARTLPLHPNGMHFVASDAAGAPLGEWT
                     VYSVGGGDLLDPEEPAADEVYEPVSMADVLAWIEQGGNSFWEYVESHEPPDIWDHLAR
                     VRDAMCASITRGLANEGTLPGVLKLPRKAPAYHIRADHASAHVRSRVLLFAYALAVAE
                     ENAAGGEIVTAPTCGSAGVLPAVLRLLSEQHGFDERKIVRALATAGLVGNLVRHNGSI
                     SGAEVGCQGEVGTACAMAAAAATQLLGGTPRQIEYAAEMGLEHHLGLTCDPVAGLVQI
                     PCIERNAFGAARALDACYFALSSDGVHRVSFDRVVRVMRQTGHDLPSIYRETSEGGLA
                     VV"
     gene            4648..>5883
                     /locus_tag="PLT35_06315"
     CDS             4648..>5883
                     /locus_tag="PLT35_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28880.1"
                     /translation="MRIAGRAFSLPNAVMGALALAWAVSMAAVAANTRPIASDDALIS
                     FEYARNLARGQGLVFNSGERVWGFTSPLHTLLLGGLASAGMDAVRAAFATAFVWIGAS
                     SVMLYRAGRDILPRPMALCLGALFLLDGTAHGDYGLESSLLVALQLAFLLAAQAGWGR
                     TASLLAAISCLARPDSLLLVAPVLLFGRETRKPRNLVWFAAVGLAWEGFALAYYGDLV
                     PNSYHAKAGLTEFRAYLANAAAHVTRLGLADPAYTGFVAAERALVVALAGLAVLNPHV
                     RQRPALAYALVPYPWLLVLAYSAIGSYRGHNWESHSARFFFLSAVAVGFLTVASQLAG
                     RWRKPAHAALVAPAALLALIALHGLFHTRELACSLTAGDRTYWGGARYDAYRQIAAWA
                     SDHLPPGSSVAISEVGTFGY"
BASE COUNT          881 a         2006 c         2099 g          887 t
ORIGIN      
        1 ccgcccgtgg ggtcgtcgac gtcgcccgca aaccgtggga tcaggtgcaa atggaagtgc
       61 atgacggtct ggccggcggc agcgccgacg ttcatgccga tgttgtagcc gtcagggcgg
      121 cgatcgtgat ccagccgttc cttgacttcg tcgaccaggg ccatcaacgc ttcgcgctct
      181 gcggcagaga cttcgaacca ggatgcgatg tggcggcgcg ggacgatgag cgcgtgtccg
      241 ggggagacgg gataggcatc gctgagggcc acggccaggg cattccccgc aatgatcgcg
      301 ccgggggctg gattacagaa cgggcaaccc gacagcactt cggtcatatg tgcggcaacg
      361 atagcacgcg acggcgctgc gtgacccccg tcaaacgcga gggccacctg cgcaacatcc
      421 ggcgcatcgg cgcgctgacg gagaagtgct accagggccg cccatgggcg cccgcggccc
      481 cgaaggccag gactggagga ggacggcccg acccgcgccg gcgtcactcc ttgcgcggca
      541 ggcgcgcgcc gcggagctcg cggctcgcgg tcgcaaccgt cgagttgtcc tgctgtccgc
      601 cgtagatgcg gtacgggaag tggttgtcga cgtcgatcca gtcgtactgc gccgtcggtt
      661 gcacgtcctg gcagttcctg ccgcagtcga gggtaatcgc cgcgccgccc tccttcagcc
      721 cggcgacgcg ggagccggat cgggatcggc cgtgatatgt tcggtctcaa ggagtcacag
      781 gcatgcaccg tcgcgtgttg tccgtactgg tcctgacgcc gatcctcgcc gccgttctgc
      841 actcatccat gactgccatg acaccacaag ccgccgcggc gggcgccgcc cgcgagaacc
      901 cgctgatgaa accgagcccg ctgccgttcg gcgcgccggc cttcgacaag atccaggacg
      961 ccgacttcaa gcccgcgatg gaagcgggta tcaaggccga gctcgaggaa gtcacggcca
     1021 tcgccgcgaa tgccgcggcg ccgacgttcg agaacacgct cgtggcgctc gagcggaggg
     1081 gagtcctcct gcggcgggtg agcctcatcc tgaacgcgct cgcctcggcg aacaccaacc
     1141 cgacgatcca ggccctgcag gaagagctcg cgccgaagct ggcggccctg gacgatgcga
     1201 tcttcctgaa cgagaagctg ttcgcgcgtg tcgaaaccgt gtacgagtcg cgggcctcgc
     1261 tcacgctcga cccggaatcg gccaggctcc tcgagttcta ctacnnnnnn nnnngggggc
     1321 cgcggacaag gcggccctca aggccctgaa cgagcaggag gccgggctca gcgcgaagtt
     1381 cgtgaaccag ttgctggctg cgacgaaggc gggggcgctg gtcgtcagcg atccggcgga
     1441 gctggccggc ctgacgaagg acgagctcga agcggcggcg atcgccgcga agtcgagggg
     1501 cctcgacggc aagtgggtgc tgccgctccg gaacacgacg cagcagggcc cgctcgcgtc
     1561 gctgacgaac cgcgccacgc gcgagaagct cttccgcgcg tcctggacgc gcgccgagcg
     1621 cggcgacgcc aacgacacgc ggcagacgat cgcgacgctg gccagcctga gggccaggaa
     1681 ggccgcgctg ctcggctatc cggacttcgc ggcctgggtg ctcgaggacc agatggcgaa
     1741 gacgcccggg gcggtgaacg ccttcctggg ccggctgatc ccgccggcca ccgcgaacgc
     1801 gcgcgcggag gcgaaggaca tccaggcgat catcgactcg cagaagggcg gcttccagct
     1861 cgcgccgtgg gactgggatc tctacgccga gcaggtccgc aaggcgaagt tcgatctcga
     1921 cagtgccacg gtgtcgcagt acctggaact cgatcgcgtg ctccgcgacg gcgtcttctt
     1981 cgcggcgcag gagctctacg gcctgtcgtt caaggagcgc cgcgacctgc ccgtctacca
     2041 gcaggacgtg cgcgtcttcg acgtgatcga ccgcgacggg tcgccgctgg cgctgttcta
     2101 cttcgacccc tacgcccgcg acaacaagaa cggcggcgcg tggatggaca acttcgtcgt
     2161 ccagtcgaag ctgctcggca cgaagcccgt cgtcttcaac tgcgcgaaca tcgcgaagcc
     2221 ggcgcccggc cagcccgtgc tgctggcgtt cgacgaggtg ctgacgctct tccacgagtt
     2281 cgggcacgcg ctgcacggca tctttgccga ccagcagtac ccgagcctgt cgggcacgtc
     2341 ggtggcgcgc gacttcgtcg agctgccctc gcagttcaac gagcattggg cgctcgacgc
     2401 gaaggtgctg tcccgctacg cgaggcacca caagaccggc gagccgatgc cgcaggcgct
     2461 ggcggacaag ctgaaggcgg cggcgacgtt caaccagggc tacagcctga cggagatcct
     2521 cggggccgcg gtgctcgaca tgcgctggca ctcgatgcct gcgggctcgg gcgagcagcc
     2581 ggccgggccg ttcgaggccg gcgtgctcga gcagtccggc ctgaacctcc cgcaggtgcc
     2641 gccgcgctac cggtcgagct acttcctgca catctgggcg aacgggtacg aggcgggcta
     2701 ctacgcgtac ctctgggccg agatgctcga caacgacgcc taccagtggt tcgtcgagca
     2761 cggcgggctg acgcgcgcca acggcgaccg cttcaggcag atgatcctgt cgagagggaa
     2821 cgctggggac tacgcgaagc tctaccgcga gttccgcggc caggatccgt ccatcgagcc
     2881 gatgctgaag cgccggggca tcagcacggc ggcggcgccg gcgaagcggc cgtaggcgag
     2941 ccggccggcg tccagcgccg gtgcgtgtcg gtcgtgttgt ccggcgcgaa cccggtgacg
     3001 gcagcgtcgg ggctgaagcc ggcaccctca gggcttggcc gcccgggcca gcttcccggc
     3061 gctccaggct gcgacctcgc ctgtcacgcc tctctcgcac acctccgcgg cgacgccgtg
     3121 gttgccgcgg cgaccgaggg actcgagctc ctggacgacg aggccgactg cgcgcggcag
     3181 atcaacatgg tcatggccag cgcagaggag atgaccaagg acctggtgaa gcgctacggc
     3241 gggaccacca ggaagagggc aggcaggaag aaggcttgag atcggcgtac agtagaaacg
     3301 gcatcctgag tggtcttcgg gcctgccaac cggcgacgga cagcgcacgg tggattcccg
     3361 ccagggagat gcggccccgg gacttggggg cggcagacgg ccatggctcg ccggagcgcc
     3421 ctcggctatc atgcgctcat gcgttccgtg aaatcgctct acaagatcgg gcacgggcct
     3481 tcgagcagcc acacgatggc gccgcggttc gccgccgagg ccttcctccg ccgtcatccc
     3541 gcggccaccc gggtgacggt caccctgtat ggcagcctgg cggccacggg caaggggcac
     3601 ctgaccgata tcgcgctccg ggaggccctc gggaagaccg ccgtcgagct gcgctgggag
     3661 cccgcgcgca cgctcccgct gcaccccaac ggcatgcact tcgttgcctc cgatgcagcg
     3721 ggcgcgccgc tgggcgaatg gacggtgtac agcgtcggcg gcggcgacct gctcgatccg
     3781 gaggagccgg ctgcagacga ggtgtacgaa cccgtcagca tggcggacgt cctggcgtgg
     3841 atcgagcagg gcgggaacag cttctgggag tacgtggagt cgcacgagcc gccggacatc
     3901 tgggaccatc tcgcccgcgt gagagacgca atgtgcgcgt cgatcacgag gggcctggcc
     3961 aacgagggca cccttcccgg cgtgctgaag ctgccgcgca aggcgccggc ctaccacatc
     4021 cgggcggatc acgcgtcggc gcacgtgcgt tcgcgggtcc tgctgtttgc ctacgcgctg
     4081 gccgtggcgg aggagaacgc ggcgggcggg gagatcgtga cggcgccgac gtgcgggtcg
     4141 gcgggcgttc tgccggcggt gcttcggctg ctcagcgagc agcacgggtt cgacgagcga
     4201 aagatcgtcc gcgccctggc cacggccggt ctcgtcggca acctcgtcag gcacaacggg
     4261 tccatctcag gcgcggaggt cggctgtcag ggcgaggttg gcacggcctg cgccatggcg
     4321 gcggccgcgg cgacgcagtt gctcgggggg acgccgcgcc agatcgagta cgcggcggag
     4381 atggggctgg agcaccacct gggcctcacc tgcgacccgg tggccggcct ggtgcagatc
     4441 ccgtgcatcg agcgcaacgc gttcggggcc gcccgggccc tggacgcctg ctacttcgcg
     4501 ctctcgtccg acggggtcca ccgggtctcc ttcgatcgcg tcgtccgggt gatgcggcag
     4561 accggccacg acctgccgag catctaccgc gaaaccagcg aaggcggcct ggccgtcgtc
     4621 tgagcggcgg ctggcacctg gctcagcgtg cgaatcgccg gccgcgcctt cagcctgccc
     4681 aacgccgtca tgggcgcgct cgcgctggct tgggccgttt cgatggcggc cgtcgcggca
     4741 aacacccggc ccatcgcgag cgacgacgcg ctcatcagct tcgagtacgc ccgcaacctg
     4801 gctcgcggcc aggggctggt cttcaattcg ggcgaacgcg tgtgggggtt cacctctccg
     4861 ctgcacacgc tccttctggg agggctcgcg tcggcgggga tggacgcggt gcgcgcggcg
     4921 ttcgccacgg ctttcgtctg gatcggcgcg tcatctgtga tgctctaccg ggccggcagg
     4981 gacatcctgc cccggccgat ggctctgtgc ctcggcgcgt tgttcctgct cgacggcacg
     5041 gcgcacggcg actacggcct cgaaagcagc ctgctcgtcg cgctacaact ggcgttcctt
     5101 ctggccgcgc aggcgggatg gggccggacg gcgagcctcc tggccgcgat atcctgcctg
     5161 gctcggcccg actcgctgct gctcgtggcg ccggtcctcc tgttcggtcg tgagacgcgg
     5221 aagccgagga atctcgtctg gttcgcggcc gtcggcctgg cctgggaggg attcgcactc
     5281 gcgtactacg gcgacctggt gccgaactcg taccatgcga aggccggcct tacggagttc
     5341 cgggcgtatc ttgcgaacgc cgccgcgcac gtcacgcgcc tcgggctggc ggacccggcc
     5401 tacaccggat tcgtggctgc ggagcgggcc ctggtggtgg cgctcgccgg gctcgccgtg
     5461 ctcaacccgc acgtgcgcca gcggccggcc ctcgcgtatg cgctggtgcc gtacccgtgg
     5521 ctgctcgtcc tcgcctacag cgccatcggc tcgtatcgcg gccacaattg ggagtctcac
     5581 tccgcgaggt tcttcttcct gtccgccgtt gccgtcgggt tcctcacggt cgcgagccag
     5641 ttggccgggc gctggcgcaa gccggcccac gcggcgctcg tggccccggc cgcgctgctg
     5701 gcgctgatcg ccctgcatgg gctgttccac acccgcgagt tggcgtgctc tcttacggcc
     5761 ggagaccgta cctattgggg cggggcacgg tacgacgcct atcggcagat cgcggcgtgg
     5821 gcgagcgatc atctgccgcc aggatcgtcg gtcgccatca gcgaggtcgg gacgttcggg
     5881 tac
//
LOCUS       DAOSIF010000145         5862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12309_length_5862_cov_3.647494, whole genome shotgun sequence.
ACCESSION   DAOSIF010000145 DAOSIF010000000
VERSION     DAOSIF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12309_length_5862_cov_3.647494"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLT35_06320"
     CDS             complement(<1..1153)
                     /locus_tag="PLT35_06320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOG28881.1"
                     /translation="MTEGPGRWSRVARLFEEARQLPRGEAGAYLESACAGDSALEGDV
                     RALLDADERADGFLEESGADVGSLLMDDAVPPLPAGTMIGWYRVERALGRGGMGQVYL
                     AEDTRLARPVALKLLHPGDTADEKRRERLRFEARAAAALRHPNVATVHALEEFPDQPC
                     IVSEYVPGRTAREVLDAGGPLPAADAIDVALQVARGLDAAHHCGIIHRDLKPENILIG
                     DDRTVKILDFGIARAMQLDPARKRLTDTGVLVGTPGYMAPEQVEGHEGDARSDVFALG
                     TVVYELVTGVHPFQGRTASSSAARVLTAEPDPPSRVNPRVPPGLDHIVARCLEKEPAG
                     RYASAADVASDLASLARTLADDPRLLRPMRALLDATPGPGVPARGLWRAH"
     gene            complement(1167..1754)
                     /locus_tag="PLT35_06325"
     CDS             complement(1167..1754)
                     /locus_tag="PLT35_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG28882.1"
                     /translation="MSDRTPQSITGLLKRASAGSQDAFDEVFPLVYAELRRIAAREMR
                     RERPGQTLQTTALVHEAYLRLLKDASLSFANRAHFLGIAARAMREILIERARARAARK
                     RGGQALRVTFDEQTLRTPPPPVDVLALDEALRRLALLDERHARVVELRYFGGLSVDET
                     AAALGVSPATVKRSWALARAWLYKELTPHDGPRPA"
     gene            complement(1940..3373)
                     /locus_tag="PLT35_06330"
     CDS             complement(1940..3373)
                     /locus_tag="PLT35_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG28883.1"
                     /translation="MDFDAPFFLAGRWERSSRALDVRNPWNGALVGRTWLADDTQVEA
                     AVSAAAAAAAAMRGLPLHERAAVLARASAALAKRREEVGRILAGEAGKPIRDALAEVD
                     RAAATLQVSGEEARRLGGEVIPMDLAPHGKGRIAILRRFPLGPVAAISPFNFPLNLSA
                     HKLGPAVAAGDPVVLKPATKTPLTGLILASVLEEAGLPAGGLSVLPMPRQLGDRLVTD
                     ERFKLLTFTGSSAVGWAMKARAGKKKVVLELGGNAGVIIDESADVPFAVNRVAAGGFA
                     YAGQSCISVQRVLVHEGVYARFAGQLVERVGSLVVGDPLDPATDVGPMIDEGEADRID
                     AWVREAVAEGARVLTGGRRLGPCAYAPTVLEGVKEDAKVCAQEVFAPLVALYPFSRFD
                     DALASVNRSRYGLQAGVFTGNLQHALQAYDRLETGGVIINDVPTYRIDHMPYGGVKDS
                     GTGREGPRYAIEEMTEPKLLVINTEVG"
     gene            3567..3890
                     /gene="erpA"
                     /locus_tag="PLT35_06335"
     CDS             3567..3890
                     /gene="erpA"
                     /locus_tag="PLT35_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOG28884.1"
                     /translation="MVNVSDSAAGKIKELLAEEQKIESGLRVFVQGGGCSGFQYGLMI
                     EDAPAPGDQSIEANGIRLFVDPISARYLDGAEVDFIDSVTGGGFTIRNPNVRTTCGCG
                     SSFNA"
     gene            3910..4872
                     /locus_tag="PLT35_06340"
     CDS             3910..4872
                     /locus_tag="PLT35_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG28885.1"
                     /translation="MSAPAGPAAADAFRPHAEGGRRASKRERILELFLTHEGRLSTDD
                     LVGLIRREDPGVSRATVYRTLRWMVDAGVARRIDFPEGPSRFEHAYRHPRHFHLICKT
                     CNRSFEFLSSEIEGVIDEVASARGFAARQSVLQIYGTCEACLAGRTPQAEPATGERVF
                     ARDALRIAIATQRSALEFYTRASRLARRARIRRVFQKLADEARDQLSLLEARYQRLLE
                     SDPDLESRPTFLFFKGAAHGLFAEGADEVAKGIDDRQALKIGTRCERLSHQFFKRYGG
                     RFDDSDSKRLFLAFADGARRRLDMLLGEYIGMGKKKVGAGRKSA"
     gene            4963..5685
                     /locus_tag="PLT35_06345"
     CDS             4963..5685
                     /locus_tag="PLT35_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28886.1"
                     /translation="MDPLVEIAGLSKSCGGRCPLRIAALSVGPAERVAIVGLDAAAAE
                     VLVNLIIGAILPAEGEVRVFGRPTSVIADADDWLATLDRFGLVSPRAVLPGGFTARQA
                     IAAAFTLSLDPLAHGVSTDVERLSREAKLPRAALDQPLEGADPLTKARCRLARALAPN
                     PAVLVLEHANALVPAHAARFGGDIASIARRRGMAVVALTADAAFARAVADRVLRLDAA
                     TGRLAETAGWPWRPGRQTARTD"
     gene            5682..>5862
                     /locus_tag="PLT35_06350"
     CDS             5682..>5862
                     /locus_tag="PLT35_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28887.1"
                     /translation="MRLLLFDVDGTLLLSGGAAVRAMDAAFHEVFGVRGAFDGMPLGG
                     RTDGSILADALERANP"
BASE COUNT          811 a         2113 c         2090 g          848 t
ORIGIN      
        1 agtgggcccg ccagagcccg cgggccggca cgccgggccc gggcgtcgca tccaggagcg
       61 cccgcatcgg gcgcaggagg cgcggatcgt ccgcgagcgt cctggccagc gatgcgagat
      121 cggacgccac gtcggcggcg ctggcatacc gcccggccgg ctccttctcg aggcaccgcg
      181 cgacgatgtg gtccaggccc ggcgggaccc tcgggttcac gcggctgggc gggtccggtt
      241 cggcggtcag gacgcgggcc gcggaagacg acgccgtccg gccctggaac gggtgcacgc
      301 ctgtcaccag ttcgtaaacg accgtgccga gcgcgaagac atcgctgcgg gcatcgccct
      361 cgtgcccctc gacctgctcg ggagccatgt agcctggcgt cccgacgagg acgcccgtgt
      421 ccgtgaggcg cttgcgcgcg gggtccagct gcatcgcgcg ggcgatgccg aagtcgagga
      481 tcttcacggt ccggtcgtcg ccgatcagga tgttctccgg cttgagatcg cgatggatga
      541 tgccgcagtg atgcgcggcg tccaggccgc gggccacctg cagcgccacg tcgatggcgt
      601 ccgccgcggg aagcggcccg cccgcatcga ggacctccct ggccgtgcgg cccggcacgt
      661 actcggacac gatgcagggc tggtccggga actcctccag cgcgtgcacg gtggcgacgt
      721 tcgggtgtct cagcgctgcg gcagcccggg cctcgaagcg gaggcgctcg cgccgcttct
      781 cgtcggcggt gtctccgggg tggagcagct tgagcgcgac cgggcgggcc aggcgcgtgt
      841 cttccgccag gtagacctgg cccatcccgc cgcggcccag cgctcgctcg acgcggtacc
      901 agccgatcat cgtgcccgcc ggaagcggcg ggacagcgtc gtccatcagc agcgagccga
      961 cgtccgcgcc ggactcctcc aggaagccgt ccgcgcgctc gtccgcgtcg aggagcgccc
     1021 gcacgtcccc ctcgagcgcg ctgtcgcccg cgcaggctga ctcgagatag gcgccggcct
     1081 ccccgcgggg gagctgccgg gcctcctcga acaggcgggc gacccgagac caccggccgg
     1141 ggccctcggt catctcgctc atcatctcac gccgggcgcg gcccgtcgtg cggcgtcagc
     1201 tccttgtaca gccaggcccg cgcaagggcc cacgatcgct tgaccgttgc gggcgacacg
     1261 cccagggcgg cggccgtttc gtcgacggac agcccgccga aataccgcag ctcgacgacg
     1321 cgagcgtggc gctcgtcgag gagggcgagg cgccggaggg cttcgtccag ggcgaggacg
     1381 tcgacgggcg gcggcggcgt ccggagggtc tgctcgtcga acgtcacgcg cagagcctgc
     1441 ccgccgcgct tccgggcggc gcgggcgcgg gcccgctcga tcaggatctc gcgcatggcc
     1501 cgcgcggcga tgcccaggaa gtgggcccgg ttcgcgaagg acagcgaggc gtccttcagc
     1561 agcctcaagt acgcctcgtg gaccagcgca gtggtctgaa gcgtctgccc cggccgctcg
     1621 cggcgcatct cgcgcgccgc gatgcggcgg agttccgcgt agacgagtgg gaacacctcg
     1681 tcgaacgcgt cctggctgcc ggccgacgcc cgcttcaaga ggcccgtgat cgactgcggc
     1741 gttctgtcgg acatgcctgt tccgcgttgc ggaaccccga tcgcttatcc cttgtccctt
     1801 gccccttagt ccttgcccct gaccgcccag cgcctagccc cttttctcct gtcctttgcc
     1861 cctcgcccct cgttcctgtc tgaccgaatc ccgaaccccg agtccgagcc ccgagccccg
     1921 agccccgagt cccgagcccc taccccacct ccgtattgat gaccagcagc ttcggctcgg
     1981 tcatctcctc gatagcgtag cggggccctt cgcgcccggt gcccgagtcc ttcacgccgc
     2041 cgtagggcat gtgatcgatg cggtacgtgg gcacgtcgtt gatgatgacg cccccggttt
     2101 cgagccgatc gtacgcctgc agggcgtgct gcaggttgcc ggtgaagacg cccgcttgga
     2161 gcccgtagcg cgagcggttc accgacgcga gggcgtcgtc gaaccgcgag aagggataga
     2221 gcgcgacgag cggggcgaac acctcctgcg cgcacacctt cgcgtcctcc ttcacgcctt
     2281 cgagcactgt cggggcgtag gcgcacgggc cgaggcgccg cccgccggtg agcacccggg
     2341 cgccctcggc gacggcttcc cggacccagg cgtcgatgcg gtccgcctcg ccctcgtcga
     2401 tcatcggccc gacatcggtg gccgggtcga gcgggtcgcc gacgacgagg ctgccgacgc
     2461 gctcgacgag ctggccggcg aaccgcgcgt agacgccctc gtgcacgagc acgcgctgga
     2521 cagagatgca gctctggccg gcgtaggcga acccgccggc tgccacgcgg ttcaccgcga
     2581 aggggacgtc ggccgactcg tcgatgatca cgccggcgtt gccgcccagc tcgaggacca
     2641 ccttcttttt gccggcgcgg gccttcatcg cccatcccac cgccgacgac ccggtgaagg
     2701 tcagcagctt gaaccgctcg tccgtcacca gccgatcgcc aagctggcgc ggcatgggca
     2761 gcacgctcaa cccgccggcg ggcaggcccg cttcttccaa cacgctcgcc aggatcagcc
     2821 cggtgagcgg ggtcttcgtc gcgggcttga ggacgaccgg gtcgcccgcg gccacggcgg
     2881 ggccgagctt gtgggccgac aggttcaggg ggaagttgaa cggcgagatc gccgcgacgg
     2941 ggccgagcgg gaagcgcctg aggatggcaa tgcggccctt gccgtgggga gccaggtcca
     3001 tcgggatgac ctcgccgccg agccggcggg cctcctcgcc ggacacctgc agcgtcgccg
     3061 ccgcgcgatc cacctccgcg agcgcgtccc ggatcggctt gccggcttcg cccgccagga
     3121 tccggccgac ctcctcgcgc cgcttcgcca gggccgccga ggcccgcgcg agcaccgccg
     3181 ctcgctcgtg cagcggcagg ccccgcatgg cggcggcggc ggcggcggcg gccgacacgg
     3241 ctgcttccac ctgcgtgtcg tcggcgagcc aggtgcggcc gaccagcgcg ccgttccacg
     3301 ggttcctgac gtcaagggcg cgtgaagagc gttcccagcg tccggcaagg aagaaagggg
     3361 cgtcgaagtc catgcgtcgc gctccttgct gcggccccgg ggatcccagg aagggagacg
     3421 agcgtcaaac tcgctgtagg ataacacgat gcgccgcccg gccttgccgc gtccgcccgg
     3481 cggcaggcct cgcgacgtgc cgggcctcgc gcctggcgcg ctataatcgg aggacgggtt
     3541 tcgacccgtt ccccaaggag cattgaatgg tcaacgtgtc tgactcggcg gcaggtaaga
     3601 tcaaggagct gctcgccgaa gagcagaaga tcgagagcgg cctccgcgtg ttcgtgcagg
     3661 gcggcggctg ctcggggttc cagtatggcc tgatgatcga ggacgcccct gcgccgggcg
     3721 accagtcgat tgaggcgaac gggatcaggc tcttcgtcga tccgatcagc gcgcgctacc
     3781 tcgacggcgc cgaggtcgat ttcatcgaca gcgtcaccgg cgggggcttc acgatcagga
     3841 atcccaacgt caggacgacc tgcgggtgcg gttcgtcgtt caacgcgtga tcgcgggggc
     3901 cgcaccccga tgtccgcgcc cgcgggtcca gccgccgccg acgccttccg tcctcacgcc
     3961 gagggcggga ggcgcgccag caagcgggaa cgcatcctcg agctcttcct gacgcacgag
     4021 gggcgcctga gcacggacga tctcgtcggc ctcatccggc gcgaggatcc gggcgtcagc
     4081 cgggccaccg tctaccgcac cctgcggtgg atggtggatg ccggggtcgc ccggagaatc
     4141 gacttccccg aaggcccgtc ccgcttcgag catgcgtacc ggcatccgcg gcacttccac
     4201 ctcatctgca agacctgcaa ccgatcgttc gagttcctca gctccgagat cgagggggtc
     4261 atcgacgagg tggcctccgc gcgcgggttt gcggcgcgcc aaagcgtgct tcagatctac
     4321 gggacgtgcg aggcatgcct ggcggggcgg acgccgcagg ccgagcccgc aaccggggag
     4381 cgcgtgttcg cgagagacgc cctccgcatc gcgatcgcca cccagcgcag cgccctcgag
     4441 ttctacacgc gcgcatccag gctcgccaga cgagccagga tccggcgcgt cttccagaag
     4501 ctcgccgacg aggcgcgcga ccagctgtca ctcctcgagg cccgctacca gcgcctgctc
     4561 gagtccgatc cggacctgga gtcccggccg acatttctct ttttcaaggg agccgcccac
     4621 gggctgttcg ccgaaggggc cgacgaggtc gccaagggga tcgacgaccg ccaggcgctc
     4681 aagatcggga cccgctgcga gcggctctcg caccagttct tcaagcggta cggcggccgc
     4741 ttcgacgact cggacagcaa gcggctgttc ctggcgttcg ccgacggcgc gcggcggcgc
     4801 ctcgacatgc tgctcggcga gtacatcggg atgggaaaga agaaagtggg agccgggaga
     4861 aagagcgcct aggatccagc agaagtcaga agtcaggggt cggaatcccc cagccctcac
     4921 tccccggccc tcggtccctg tcgtacccgc tacaatctgt tcgtggaccc gctcgtcgag
     4981 attgccgggc tgagcaagag ctgcggcggg cgttgcccgc tgcggatcgc ggcgctctcg
     5041 gtgggcccag ccgagcgtgt ggccatcgtc ggcctggacg cggcagcggc ggaggtgctc
     5101 gtcaacttga tcatcggcgc catcctgccc gccgagggcg aggtgcgcgt cttcgggcgc
     5161 ccgacgtcgg tgatcgccga cgccgatgac tggctggcga ccctggaccg tttcggtctc
     5221 gtgtcgccca gggccgtgct gccaggcggc ttcaccgcgc gccaggcgat cgcagcggcc
     5281 ttcacgctgt cgctcgatcc cctcgcgcac ggcgtctcaa cggatgtcga gcgcctctcc
     5341 cgcgaagcga agctcccgcg agcggcgctg gatcagccgc tcgagggcgc cgatcctctc
     5401 acgaaggcgc gctgccggct ggcgcgggcg ctcgcgccga atccggccgt gctcgtgctc
     5461 gagcacgcaa acgcgctggt tccggcgcat gcggcgcgct tcggcggcga catcgcgtcg
     5521 atcgcccgga ggcgggggat ggcggtcgtc gcgctgaccg ccgacgcggc gttcgcgcgc
     5581 gcggtggcag accgggtgct ccggctggac gcggcaaccg gccgcctggc agagacggct
     5641 ggctggccct ggcggcccgg ccggcagacg gcgaggaccg attgaggctc ctgctcttcg
     5701 acgtggacgg gacgctgctg ctctcgggag gcgcggccgt gcgcgcgatg gacgcggcct
     5761 tccacgaggt gttcggcgtc cgcggcgcct tcgacgggat gccgctcggc gggcgcacgg
     5821 acgggtccat tctggcggac gcgctggagc gcgcgaaccc tt
//
LOCUS       DAOSIF010000146         5827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12391_length_5827_cov_2.893971, whole genome shotgun sequence.
ACCESSION   DAOSIF010000146 DAOSIF010000000
VERSION     DAOSIF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12391_length_5827_cov_2.893971"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="PLT35_06355"
     CDS             <1..1788
                     /locus_tag="PLT35_06355"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOG28888.1"
                     /translation="DSQWFGGRAEGWERAPYWLDGVIPLAWILDDGPLKAPITGYVDF
                     IVAHQRSDGWFGPYPEDAVTKRYDLWAILLANKALAQYHEATGDSRVLEAIVKSLRAL
                     LVGLDRTPLYGWGRFRWFEGLVPAFHAYQHTREPWLLDLALKLREQGVDFEALYRTDD
                     IARPTPRRGLWKWTKHVVNTAMAAKAAALNWRLDRRPADRAFAAAMIETLDRHHGQVT
                     GMFSGDECLAGTSPLQGTELCAVVEFMYSLEHLFSVFGDAPVGDRLERVAFNALPATF
                     APDMWSHQYDQQVNQVQCTVNPDHMWTTNGPESNLFGLEPNYGCCTSNMHQGWPKFAA
                     HLWMRTPDEGLVAAAYAPSVARFTARGVPVTIALDTDYPFREDLAITVEAAAAVRFPL
                     VLRVPQWARDAAIRVGGLPERRLVPGTLHRVEREWRGKTAVSLRFPMRPAVTVRDNGA
                     VAVERGPLVYALRIGEEWRRVNADKPHRELPHGDFEVRPSTPWNYGLVLDEGRPETGL
                     IFEERPVGERPFSPEGAGMAARATGRRIPSWTIAHGWAAEISPPDLAWSDPARSPSDE
                     PDEEVTLIPYGCTNIRVTEFPRLRPRRKA"
     gene            1808..2824
                     /locus_tag="PLT35_06360"
     CDS             1808..2824
                     /locus_tag="PLT35_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOG28889.1"
                     /translation="MTDRSRISWRTLAVVSAFVAALALASAGCGDKTTVLTAPSPQLA
                     IACPDSQTAMAPSGQPVAVSWPAPATSGGTAPVRTTCTPSSGSSFGLGSTTVACSATG
                     AARGETASCTFTVTVHPSRLSVTTFMAYGDSITWGKKSDPVAFVSDMEPENDYSYPNV
                     LRRLLAAGYPGQQFTVLNEGKPGEAVAAGLSRLPGELALGAPDVLLLLDGANDLLGSP
                     GSATTEYIAGQLRQMVRTAKAQRPGMAVLLANFPPQWHGSVPYDRGAGAEFVPELNQR
                     IASLAAAEGATLVDLYGPLSADIKRYIGADGLHPTEAGFALMAETFYAAIRRTFETTT
                     AGRR"
     gene            2901..5150
                     /locus_tag="PLT35_06365"
     CDS             2901..5150
                     /locus_tag="PLT35_06365"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOG28890.1"
                     /translation="MNVTRLGVLTSGGDASGMNAAVRAIVRTALDNGLEVWGIFEGYR
                     GLIEGGDMIRPLGWRDVGGVIHRGGTFIGSARSEAFRTREGRLAAARNLLQRGIDGLV
                     VIGGDGSLTGADVLRREWPDFVRELEASGAVEPGAAARHPRLVVVGLVGSIDNDAAGT
                     DMTIGADTALHRITEAIDAIGSTASSHRRAFVVEVMGRHCGYLALMAAIATGADAVFI
                     PEDPPPDDDWERRLVGQIVAGRQAGRRHSTVVVAEGATDRHGAAITSERVREALSGGL
                     GEAVRVTVLGHVQRGGAPSAFDRTLGTIMGHAAIETVLSGEADERALVMGMRGNRAVR
                     IPLEACVADSRAVNECLSSRQYARALDLRGPGFNDSLATYRTLVRALPRPPREGQRRL
                     RLAVLHAGAPAPGMNAAVRAAVRLGLDDGHQMLGVRRGVHGLVGADLVAMDWMSVNGW
                     APMGGAELGTSREVPAGPDLYAIARTIERESLDGLLFIGGWEAYETAHRLFEERANYP
                     AFGVPIVCLPATIDNNLPGTELSIGADTALNNIVDVVDKIKQSAVAEQRCYVVEVMGR
                     RCGYLALMSGLATGAERVYLHEEGVTLQRLVDDLAVLVQGFSSGKRLGLMIRSERAND
                     VYDTRFMCALFEQEGGGLFDVRESILGHLQQGGDPSPFDRIQATRLARLGIGRVASEA
                     LAGRAPAEFVGMVSGMVEFHSFEDYVRLVDAPNRRPKQQWWLGLRTINEALARPAPRG
                     AVRDAPLPR"
     gene            complement(5125..>5827)
                     /locus_tag="PLT35_06370"
     CDS             complement(5125..>5827)
                     /locus_tag="PLT35_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOG28891.1"
                     /translation="LRAARRGETAVSQRDLTDALEKVQLGTARKVVIPEAERRRTAYH
                     EAGHALLGMLQPGADPVRKVSIVPRGRALGVTLSTPESDRYGYDANYLRGRIIGALGG
                     MAAEQEVFGVVTTGAESDLEMVTRIARSMVGRWGMSERIGTLSVLPSEGDPRMAGVSD
                     ALLNAVDEEIRRITDECYAEARRLLRENRGKLDAIVARLLERESLDEPEIYDAAGIAR
                     PSSAPAPVIEGAARP"
BASE COUNT          802 a         2086 c         2116 g          823 t
ORIGIN      
        1 gacagccagt ggttcggagg cagggccgag ggatgggagc gcgcgcccta ctggctcgac
       61 ggcgtcatcc cgctcgcctg gattctcgac gacggtcccc tgaaggcccc gatcaccggc
      121 tacgtggatt tcatcgtcgc gcaccagcga agcgacggct ggttcggacc gtacccggag
      181 gacgccgtca cgaagcggta cgacctgtgg gcgattctgc tcgccaacaa ggcgctcgcg
      241 cagtaccacg aggccacggg cgactcgcgc gtccttgagg cgatcgtgaa gagcctgcgc
      301 gccctgctcg tcggcctcga caggacgccg ctctacgggt gggggcggtt ccgctggttc
      361 gaagggctcg ttccggcctt ccacgcgtac cagcacacgc gggagccgtg gctcctggac
      421 ctcgcgctca agcttcgcga gcagggcgtg gatttcgagg cgctctaccg gacagacgac
      481 atcgcgcgcc cgacgccgcg gcgcggcctc tggaagtgga cgaagcacgt cgtcaacacg
      541 gccatggccg caaaggcggc cgcgttgaac tggcggctgg atcggcgccc ggcggaccgg
      601 gcgttcgcgg cggcgatgat cgagacgctc gaccgccatc acggccaggt gaccggcatg
      661 ttctctgggg acgagtgcct cgccggcacg agccccctcc agggcacgga actgtgcgcg
      721 gtggtcgagt tcatgtactc gctcgagcac ctgttctcgg tgttcggcga tgcgcctgtt
      781 ggcgaccgcc tcgagcgcgt ggcgttcaac gcgctgccgg ccacgttcgc gcccgacatg
      841 tggtcgcacc agtacgacca gcaggtcaac caggtgcagt gcaccgtcaa tcccgaccac
      901 atgtggacca cgaacgggcc tgagtcgaac ctgttcgggc tcgaaccgaa ctacgggtgc
      961 tgcacgtcga acatgcacca gggatggccg aagttcgccg cgcacctctg gatgcggacc
     1021 cccgacgaag ggctcgtcgc cgcggcctac gcgccgagcg tcgcccgctt cacggccagg
     1081 ggcgtgccgg tcacgattgc gctcgacacg gactacccgt tccgcgagga ccttgccatc
     1141 acggtggagg cggccgccgc cgtgcgcttc ccgctcgtgc tgcgcgtccc gcagtgggcg
     1201 cgcgatgcgg caattcgcgt cggaggcctg cccgagcgcc ggctggttcc cggcacgctg
     1261 caccgggtgg aacgcgagtg gcgcgggaag accgccgtat cgcttcgctt cccgatgcgg
     1321 ccggccgtca cggtgcgcga caacggagcc gtcgccgtcg aacgcggccc gctcgtctac
     1381 gcgctgagaa tcggggagga gtggaggcgg gtcaacgccg acaaacccca ccgcgaactc
     1441 ccgcacggtg acttcgaggt ccggccgtcg actccgtgga actacggcct cgtcctggac
     1501 gagggccgcc ccgagaccgg actgatcttc gaggaacggc ccgtgggcga gcggcccttc
     1561 tcgccggagg gagccggcat ggccgcacgc gcgaccggcc gccgcatccc gtcgtggacg
     1621 attgcgcacg gctgggccgc cgagatctcc ccgccggacc tcgcctggtc cgacccggca
     1681 cgcagcccgt ccgacgagcc ggacgaggag gtgacgctca tcccgtacgg ctgcacgaac
     1741 atccgggtga cggaattccc gcggctgcgc cccaggcgca aggcgtagct gcgctatcat
     1801 gccgtagatg accgaccggt cccgaatctc gtggcggacg cttgccgtgg tttccgcctt
     1861 cgtcgcggcg ctggccctgg cgtcggccgg gtgcggagac aagacgacgg tcctgacagc
     1921 cccttcgccg cagctcgcga ttgcctgccc cgactcgcag acggcgatgg cgccctcggg
     1981 tcagccggtc gcggtctcgt ggcccgctcc cgccacctcg ggcggcacgg cgcctgttcg
     2041 gacgacctgc acgccctcgt cgggctcgtc attcgggctc ggatccacga ccgtcgcgtg
     2101 ctcggcgacg ggcgccgccc gcggcgagac cgcgtcctgc acgttcacgg tcaccgtcca
     2161 cccgtcccgg ctctcggtga cgacgttcat ggcgtacggg gacagcatca cgtggggcaa
     2221 gaagtccgat ccggtggcgt tcgtctccga catggaaccg gagaacgact actcgtatcc
     2281 gaacgtgctc aggcggctcc tggctgcggg gtaccctggg cagcagttca ccgtgctcaa
     2341 cgaaggcaaa cccggcgaag ccgttgcagc gggcctctcg cgcctgccgg gcgagctggc
     2401 gctcggcgcg cccgacgtgc tgctgctgct tgacggcgcg aacgacctgc ttggcagccc
     2461 gggcagcgcc acgacggagt acatcgccgg ccagctccga cagatggtgc ggacggcgaa
     2521 agcccagcgg cccgggatgg cggtgctgct tgcgaacttc cccccgcagt ggcacgggtc
     2581 agtcccctac gatcgcggcg cgggcgcgga gttcgtgccg gagctgaacc agcgcatcgc
     2641 gtcgctggcg gcggccgaag gcgccacgct ggtggatctc tacgggccgc tttcggccga
     2701 catcaagcgg tacatcggcg ccgacggcct gcacccgacc gaagcgggat tcgcgctgat
     2761 ggccgagacg ttctacgcgg cgatccggcg gacgttcgaa acgacgacgg ccggcaggcg
     2821 gtgacgcgga cgcgggcccg cgccggcgcc cgcgcggcgc gtggccgccg gcacacaccc
     2881 gcgcggaggc agacagcacc gtgaacgtga cgcgcctcgg cgttctgacg agcggcggcg
     2941 acgcttcggg catgaacgcg gccgtgcggg ccatcgtccg caccgcgttg gacaacggcc
     3001 tcgaggtgtg gggcatcttc gagggctacc gcggcctcat cgagggcggc gacatgatcc
     3061 gcccgctcgg gtggcgtgat gtcggcggcg tgatccaccg cgggggcacg ttcatcgggt
     3121 cggcccgatc cgaggcgttc cgcacgcgcg aaggccggct cgcggcggcg cggaacctgc
     3181 tgcagcgcgg catcgacggc ctcgtcgtca tcggcggcga cggatcgctg acgggcgccg
     3241 acgtgctccg ccgcgaatgg cccgacttcg tccgcgagct ggaggcgtcc ggcgccgtcg
     3301 agcccggggc cgccgcgcgc catccgcggc tggtcgtcgt ggggctggtg gggtcgattg
     3361 acaatgacgc cgccggcacg gacatgacca tcggggccga caccgccctg caccggatca
     3421 ccgaagccat cgacgccatc ggcagcaccg cgtcgagcca ccgccgcgcg ttcgtcgtcg
     3481 aggtcatggg ccgccactgc gggtatctcg cgctcatggc cgccatcgcc acaggcgcgg
     3541 acgcggtgtt catcccggaa gatccacctc cggacgacga ctgggagcgc cgcctcgtgg
     3601 gccagatcgt cgcggggcgg caggccgggc gacgccactc gacggtggtc gtggccgagg
     3661 gcgcgacgga tcggcacggc gcggccatca cgagcgagcg ggtgcgggag gcgctgagcg
     3721 gggggctcgg cgaggcggtg cgcgtcacgg tgctgggcca cgtgcagcgc ggcggcgccc
     3781 cgagcgcgtt cgaccgcacg ctgggcacga tcatgggcca cgccgccatc gagaccgtgt
     3841 tgtcgggcga ggccgacgag cgcgcgctcg tgatgggcat gcggggcaac cgcgcggtcc
     3901 ggattcccct cgaggcgtgc gtcgccgact cgcgggccgt caacgagtgc ctgtcgtcgc
     3961 gccagtatgc ccgggcgctg gacctgcgcg gccctgggtt caacgactcc ctcgcgacct
     4021 accgcacgct cgtgcgggcc ctgccgcgcc cgcctcgcga gggccagcgg cgcctgcgcc
     4081 tcgcggtcct gcacgcgggc gcgccggcgc cgggcatgaa tgccgcggtg cgcgccgccg
     4141 tgcgcctggg cctcgacgac ggccaccaga tgctcggcgt gcggcggggc gtccacggcc
     4201 tggtgggcgc cgacctcgtc gccatggact ggatgagcgt caacggctgg gccccgatgg
     4261 gcggcgccga gctgggcacg tcgcgcgagg tgccggccgg cccggatctc tacgccatcg
     4321 cccgcacgat cgagcgcgag tcgctcgacg ggctgctgtt catcggcgga tgggaggcgt
     4381 acgagacggc gcaccgcctg ttcgaggagc gggcgaacta ccccgccttc ggggtcccga
     4441 tcgtctgcct gccggccacg atcgacaaca acctccccgg caccgagctg agcatcggcg
     4501 ccgacacggc cctgaacaac atcgtcgacg tcgtggacaa gatcaagcag tcggccgtgg
     4561 ccgagcagcg ctgctacgtg gtcgaggtga tgggccggcg ctgcggctat ctggcgctca
     4621 tgagcggact ggcgaccggc gccgagcgtg tgtacctgca cgaggaaggc gtcaccctgc
     4681 agcggctggt ggacgatctc gccgtgctcg tgcaggggtt ctcctcgggg aaacgcctgg
     4741 ggctgatgat ccgcagcgag cgggcgaacg acgtctacga cacgcgcttc atgtgcgcgc
     4801 tgttcgagca ggaaggcggc ggcctgttcg acgtgcgcga gagcatcctc gggcacctgc
     4861 agcagggcgg ggacccgtcg ccgttcgacc gcatccaggc cacccgcctg gcccgcctcg
     4921 gcatcggccg ggtggcgtcg gaggcgctgg ccgggcgggc cccggccgag ttcgtcggca
     4981 tggtgtcggg gatggtcgag ttccactcgt tcgaggacta cgtccgcctc gtcgacgcgc
     5041 ccaaccgccg cccgaagcag cagtggtggc tcggcctgcg gaccatcaac gaggcgctcg
     5101 cgcggccggc cccgcgcggg gccgtcaggg acgcgccgct ccctcgatga cgggcgccgg
     5161 cgcgctggag ggccgcgcga tgccggcggc gtcgtagatc tcaggctcgt ctagactctc
     5221 gcgctcgaga agcctggcga cgatcgcgtc gagcttgccc cggttctccc gcaggagccg
     5281 ccgcgcctcg gcgtagcact cgtcggtgat gcgccggatc tcctcgtcca cggcgttcag
     5341 gagcgcgtcc gagacgcccg ccatgcgcgg atcgccctcc gacggcagca ccgacagcgt
     5401 gccgatgcgc tcggacatgc cccaccgccc aaccatcgac cgtgcgatgc gggtcaccat
     5461 ctcgaggtcg ctttccgcgc ccgtcgtgac cacgccgaac acttcctgct cggcggccat
     5521 gccgccgagc gccccgatga tgcgcccgcg caggtagttc gcgtcgtacc cgtagcggtc
     5581 cgattccggc gtcgacagcg tcacgccgag ggcgcgcccg cggggcacga tggagacctt
     5641 ccgcaccggg tccgcgccgg gctggagcat gccgagcagc gcgtgtccgg cttcgtggta
     5701 ggccgtgcgc cggcgctcgg cctcgggaat gacgaccttg cgggcggtgc caagctggac
     5761 cttttcgagc gcgtcggtca gatcgcgctg gctgaccgcg gtctcgccgc gccgcgccgc
     5821 ccgcagc
//
LOCUS       DAOSIF010000147         5824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12404_length_5824_cov_3.575836, whole genome shotgun sequence.
ACCESSION   DAOSIF010000147 DAOSIF010000000
VERSION     DAOSIF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12404_length_5824_cov_3.575836"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLT35_06375"
     CDS             <1..973
                     /locus_tag="PLT35_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG28892.1"
                     /translation="GAVGLEVRMVGERVLVTRVVPGSPAADAGVRAGWEIAAVGGRDI
                     RPELRAIARQRAGRPDRGAYLAAAVRGLLAGPVGSRVTVMMRDGGNRARRLDLRRAPP
                     EGWASRPFGHFPSVAVSFRRDRLGARIGYVAFNAFLDPARLMPLFNDAVESFLDAEGV
                     VIDLRGNMGGLGDMLPGMAGWFIGAKGTSLGTIVTRAGSLKLAVTPRPSPFTGRVAVI
                     VDDLSMSAAEVMAQGLKDAGRARVFGGRTAGAVLASTVERLPNGDGFQYPIARFEFPS
                     GAVVEGVGVTPDVEVRPTRETLLEGRDVALEAAVAWLGQQNPRGQRP"
     gene            970..1791
                     /locus_tag="PLT35_06380"
     CDS             970..1791
                     /locus_tag="PLT35_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28893.1"
                     /translation="MTKRIAIVCVAGFLLGSALWRPALAQTPVVMPPAAPAVAPAQAP
                     PAGALPSGGDVLARYVDATGGTAAYDAVRNRVVRARMEIQGAGIVFHVTVYAARPASV
                     LTLIDSDATGHMESGVWNGVVWENSALRGPIVKDGAERDDTLRDAAFDHMARWKEHFT
                     SVECTGVADVNGNPAYRVVATPKIGSRQTLYFDRETALLVRSESTIDSAAGQIAVTAE
                     PGDYRKVGGILTPFTSRVSLLGQQRTLTVETIEQNVDLAADRFDPPAEIKALLRK"
     gene            1887..3419
                     /gene="xylB"
                     /locus_tag="PLT35_06385"
     CDS             1887..3419
                     /gene="xylB"
                     /locus_tag="PLT35_06385"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOG28894.1"
                     /translation="MGRCFIGIDSGTQSTKAVLVDGGSGRVIAAASNAYDILPSAVPG
                     AKEQDPADWIRAASAAIESVLREAAPEARSVAAIGISGQQHGFVPLDAEGQVIRPAKL
                     WCDTSTAPQCETLLARLGGPAGAIAKLGNTIAAGFTASKILWLKEHEPENFSRLATVL
                     LPHDYLAYWLTGRAHMEWGDASGTALMDVRTRAWRAEALEAIDAGLASKLPPLAPSIE
                     PVGTVRPSVAARFGLGPRVFVSGSGDNMMGAVGTGNVSDGIVTASLGTSGTVYACSDA
                     PIVDPNGEVAAFCDATGRWLPLACTMNVTVATEMVRTMFGLDHAGLSAAAAAAPPGSE
                     GLLLVPFFEGERTPNVPDGTGVWIGVRPRTSGPAHMARAAMEGVTLGLNYGLNRLREL
                     GLAPREIRLTGGGSKSAVWRQIAADVFGCSVVCPASEEGAAYGAALHAMWVWHHATGS
                     PRPIADIAQQFVALDETTRAMPDARTAAVYLEMQALFDRSARDLGGVFTAHRRLLTRG
                     RV"
     gene            complement(3540..>5824)
                     /locus_tag="PLT35_06390"
     CDS             complement(3540..>5824)
                     /locus_tag="PLT35_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28895.1"
                     /translation="VTPDSAHAANSAAGLLARATGAPVAASAPGAEPGPGVFVIAAPP
                     AAAGEPAPAGGLPTARVDLRLAADGSGRLASAGGRFLYAAASWLVDVAASRDLGSLAT
                     GVSVSPAFAWNRSCYDIFLAQEARIQHRLDPESYVRQLAAWGYTHVEVNALAYPMGLE
                     TGPAGEAYPMFYTYCPALDQFVASSLNRGLYPAYYLSANLAVLKRNAELARKYGLVPG
                     LLSFEPRSVPEEFFARYPMLRGPRVDHPFRSFRPRYTMTLAHPRVREHYAEMMAALMR
                     EVPDLGFLDVWSNDSGSGFEHTKSLYVGRNGGPYLIREWKDDEEIARQAGACALRFFG
                     VLKDAARAVNPAFRVITRLESFYGEHDTVWAGLGEGLDVETTSLVARGWEMPYRHPSY
                     PDSHAVNSGTVYQLEFADRERELAAELRDRGAEPHFYFSAGPHQMFAPLLGVPYPRLT
                     HRRLALLRENGAGALANVGGTCPPELVPFNVNHEILRRFQFDSDLDIDAAVSGLARAW
                     AGQERGPILQRAWDLAEEAILAFPNPAPLYSTIGFTWYRLWARPLVPDIEAIPAGERA
                     YYERFMCTTPHNPNNVDLSRDVLFTLLTPEQSAIDMARIDVRVRPPLDRAIALLGAAR
                     EEARRALGPLNVVEDQWVRLRALRCWMMTLRNVSAWVAGVHGWMRAESTHDKARWRAV
                     LDEMIASEIANSRDLADVVDSGVEFMATTALGESPLVHGRNLKALLARRVVLMERHAG
                     DDPFIDAGFMERMASRECGA"
BASE COUNT          850 a         1971 c         2177 g          826 t
ORIGIN      
        1 aggggccgtg ggcctcgagg ttcgcatggt cggcgagcgc gtcctcgtca cgcgggtcgt
       61 gccaggctca ccggcagcgg acgccggcgt tcgcgcgggg tgggagatcg cggccgtcgg
      121 cggccgggac atccggccgg agttgagggc catcgcgcgc cagcgcgccg gaaggccgga
      181 ccggggtgcg tacctggctg cagcggtgcg agggctgctg gccgggcccg tgggcagccg
      241 cgtgacagtg atgatgcgcg acggaggcaa ccgggcgcgc aggctcgatc tgaggcgcgc
      301 gccgccggaa ggctgggcgt cccgcccctt cggccacttc ccgtcggtgg cggtgtcgtt
      361 ccggcgcgac cggctaggcg cgcgcatagg ctatgtcgcc ttcaacgcct tcctggaccc
      421 cgcgcgcctg atgcccctct tcaacgacgc cgtcgagtcg ttcctcgacg ccgagggggt
      481 cgtcatcgac ctgcgcggca acatgggcgg gctcggcgac atgctcccgg gcatggccgg
      541 atggttcatc ggcgccaagg gcacatccct cggcaccatc gtcacccgag ccggctcgct
      601 caagctcgcg gtcacgccgc ggccgtcgcc gttcacgggg cgggtcgcgg tgatcgtcga
      661 cgacttgtcg atgtcagccg ccgaggtgat ggcgcagggc ctgaaggacg ccggccgggc
      721 ccgggtgttc ggcggccgga cggcgggcgc cgtgctggcg tcgacggtcg agcggctgcc
      781 caatggagat gggttccaat acccgatcgc gcgcttcgag ttcccctccg gcgcggtcgt
      841 ggagggcgtc ggggtgacgc cggacgtcga agtccggcca acgcgcgaga ccctgctcga
      901 aggccgcgac gtggcgctgg aggcggccgt cgcgtggctc ggacagcaga acccgagagg
      961 acagaggcca tgacgaagcg gattgcgatc gtatgtgttg cgggcttctt gctgggatca
     1021 gcgctctggc gcccggccct ggcccagacg cccgtcgtga tgccgccggc agccccggcc
     1081 gtcgcgcccg cgcaggcgcc cccggccggc gcgcttccgt cgggcggcga cgtcctggcg
     1141 aggtacgtcg acgcgacagg agggacggcc gcctacgacg ccgtccgaaa ccgcgtggtt
     1201 cgcgcgcgca tggagatcca gggcgccggc atcgtctttc acgtgaccgt gtacgccgcg
     1261 cggcccgcga gcgtcctcac cctcatcgac tccgacgcga cgggccacat ggagagcggg
     1321 gtgtggaacg gcgtggtctg ggagaactcc gcgctgcgcg gcccgattgt caaggacggc
     1381 gccgagcgcg atgacaccct gcgcgacgcg gcgttcgacc acatggcgcg ctggaaggag
     1441 cacttcacga gcgtcgagtg caccggcgtg gccgacgtca acgggaatcc ggcctaccgc
     1501 gtcgtcgcga cgccgaagat cggatcgagg cagacgcttt acttcgaccg ggagacagcg
     1561 ctcctggtgc gctccgagag cacgatcgat tcggccgcgg ggcagatcgc ggtgacggcc
     1621 gagccgggcg actaccgaaa ggtgggcggc atcctgacgc cattcacgag ccgcgtgagc
     1681 ctcctcggcc agcagcgcac gctgactgtg gagacgatcg agcagaacgt cgacctcgcg
     1741 gcggaccgct tcgacccgcc ggccgagatc aaggccctgc tcaggaagta gagaggagcg
     1801 aggctgcccg gcagcgcgcc gggcccgctg gtggctctgg cgccggcgcc tgtgccgcca
     1861 tcaccgtctg tctgcgaggt cgcgacatgg gcaggtgctt catcggcatc gacagcggga
     1921 cgcagtccac gaaagccgtg ctcgtcgacg gcggcagcgg ccgggtcatc gcggccgcgt
     1981 cgaacgcgta cgacatcctc ccgtccgccg tgccgggggc gaaggagcag gacccggccg
     2041 actggatccg cgcggcatcg gccgcgatcg agagcgtgct gcgggaggcg gctccggagg
     2101 cccgctcggt ggcggccatc ggcatctcgg gacagcagca cggattcgtt ccgctggatg
     2161 ccgagggcca agtcatccgg ccggcgaagc tgtggtgcga cacctcgacg gcgccgcagt
     2221 gcgaaacgct cctcgcgcgg ctgggcgggc cggccggggc gatcgcgaag ctggggaaca
     2281 ccatcgcggc cgggttcacg gcatcgaaga tcctctggct gaaggagcac gagcctgaga
     2341 acttctcgcg cctcgcgacg gtgctcctcc cgcacgacta cctggcgtac tggctgaccg
     2401 gacgcgcgca catggagtgg ggcgacgcct cggggacggc gttgatggac gttcggacgc
     2461 gcgcgtggcg cgccgaggcg ctggaggcca tcgacgccgg gctcgcgtcg aaactgccgc
     2521 cgctcgcgcc gtcgatcgag ccggttggca ccgtgcggcc gtcggtggcg gcgcggttcg
     2581 gcctcggccc ccgcgtgttc gtgtcgggga gcggcgacaa catgatgggg gccgtcggca
     2641 ccggcaacgt ttcggatggc atcgtgacgg ccagcttggg cacatcgggg accgtctacg
     2701 cctgctccga cgcgccgatc gtggatccaa acggggaggt cgcggccttc tgcgacgcca
     2761 ccgggcggtg gctgccgctg gcgtgcacga tgaacgtgac ggtggccacc gagatggttc
     2821 ggacgatgtt cggtctcgat cacgcaggcc tttctgcggc ggcggcggcg gctccgccgg
     2881 gctcggaggg cctgttgctc gtgccgttct tcgaaggcga gcgaacgccc aacgtgcccg
     2941 atggcacggg cgtctggatc ggcgtccggc cgcggacctc cggaccggcg cacatggcgc
     3001 gcgccgccat ggaaggcgtg accctcgggc tgaactacgg cctcaaccgg ctgcgggaac
     3061 tggggctcgc gccgcgcgag atccggctca cgggcggcgg atcgaaaagc gcggtgtggc
     3121 gccagatcgc cgccgacgtg ttcggctgct cggtcgtgtg cccggcgagc gaagagggcg
     3181 cggcctacgg tgcggcgctg cacgccatgt gggtctggca ccacgcgact gggtcgccgc
     3241 ggcccatcgc ggacatcgcg cagcagttcg tggcgctcga cgagacgacg cgtgcgatgc
     3301 cggacgcgcg aaccgccgcc gtgtacctcg agatgcaggc gttgttcgac cggtcggcga
     3361 gggatctcgg cggcgtcttc acggcacacc gccgcctcct gacaaggggt cgggtctaat
     3421 tatgcaagcg cccccggtgg gattgcagtt tcgcaatttc accgaagcgt cccggcggta
     3481 ctaacggttc tgaaatcggc accagtggac ttgcagaatt cgtcccgacc cctgactgtt
     3541 taggcgccgc actcgcggct tgccatgcgt tccatgaacc cggcgtcgat gaacgggtcg
     3601 tcgccggcgt ggcgttccat caacaccacg cggcgcgcca gcagcgcctt gagattgcgt
     3661 ccgtgcacga gcggcgactc acccagcgcg gtcgtcgcca tgaactcgac cccggaatcg
     3721 acgacgtcgg cgaggtcgcg gctgttggcg atctcgctgg cgatcatctc gtccagcacc
     3781 gcgcgccacc gcgccttgtc gtgcgtcgac tcggcccgca tccagccgtg aacgccggcg
     3841 acccaggccg acacgttccg gagcgtcatc atccagcacc ggagggcccg cagccgcacc
     3901 cactggtcct cgacgacgtt gagcgggccg agggccctcc gcgcctcttc ccgtgccgcg
     3961 ccgagcagcg cgatggcgcg atcgagcggc ggccgcacgc ggacgtcgat gcgcgccatg
     4021 tcgatggcgc tctgctcggg cgtcaggagc gtgaacagca cgtcgcggct caagtcgacg
     4081 ttgttcgggt tgtgcggcgt cgtgcacatg aagcgctcgt agtaggcacg ctctccggcc
     4141 ggaatagcct cgatgtcggg gacgaggggc ctggcccaga ggcggtacca cgtgaatccg
     4201 atggtcgagt agaggggcgc cggattggga aacgcgagga tggcctcttc ggcgaggtcc
     4261 caggcccgct gcaggatcgg cccgcgctcc tgtccggccc acgcgcgcgc cagtccggag
     4321 acggcggcgt cgatgtcgag gtccgagtcg aactggaaac ggcggaggat ctcgtggttc
     4381 acgttgaacg gcaccagttc gggcggacac gtgccaccga cgttcgcgag cgcgccggcg
     4441 ccgttttcgc ggagcagcgc caggcgccga tgggtcagcc gcgggtacgg cacgccgagg
     4501 agcggcgcga acatctggtg cgggccggcg gagaagtaga agtgcggttc cgcgccgcgg
     4561 tcgcgcagtt cggcggcgag ttcccgctcc cgatccgcga actcgagctg gtagacggtt
     4621 ccgctgttga cggcgtgcga gtcggggtag ctcggatggc ggtagggcat ctcccatccg
     4681 cgggcgacaa gcgaggtggt ctcgacatcg aggccctccc cgagaccggc ccacaccgtg
     4741 tcgtgctcgc cgtagaacga ctcgagccgc gtgatgacgc ggaacgccgg attgaccgcg
     4801 cgggccgcgt ccttcagcac gccgaagaac cgcagcgcgc acgctcccgc ctgccgcgcg
     4861 atctcttcgt cgtccttcca ttcgcggatg agataggggc cgccgttgcg gccgacgtac
     4921 agcgacttcg tgtgctcgaa gcccgagccg ctgtcgttcg accagacgtc gaggaagccc
     4981 aggtccggga cctcgcgcat gagcgcggcc atcatctcgg cgtagtgctc gcgcacccgc
     5041 gggtgcgcga gcgtcatcgt gtaccgcggg cgaaacgacc ggaacgggtg gtccacgcgc
     5101 gggccccgca gcatcgggta ccgggcgaag aactcctcgg gcaccgagcg cggctcgaag
     5161 gagagcagcc cgggcaccag cccgtacttg cgcgcgagct cggcgttccg cttgaggacc
     5221 gcgaggttgg ccgagagata gtaggccggg tagaggcccc ggttgagcga agacgccacg
     5281 aattgatcga gcgcggggca atacgtgtaa aacatgggat aggcctcgcc ggccggaccc
     5341 gtctcgaggc ccatcggata ggcgagggcg ttgacctcca cgtgcgtgta gccccacgcg
     5401 gcgagctggc gcacatacga ctccggatcg aggcggtgct ggatgcgcgc ctcctgcgcc
     5461 aggaaaatgt catagcagga gcggttccag gcaaaggccg gcgagacgga gacgccggtg
     5521 gccagcgacc cgaggtcgcg gcttgccgcg acgtcgacga gccaggacgc cgcggcgtag
     5581 aggaaccggc cgccggcgct tgcaaggcgt ccggacccgt cggccgccag ccggagatcg
     5641 acccgcgccg tgggcaggcc tccggccggc gccggctcgc cggctgcggc tggcggtgcg
     5701 gcgatgacga acacgcccgg cccaggctcg gcgccaggcg ccgaggcggc gactggcgcg
     5761 ccggttgcgc gcgcgagcag gcccgccgcg ctgttggcgg cgtgcgcgga atccggcgtc
     5821 accc
//
LOCUS       DAOSIF010000148         5818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12418_length_5818_cov_4.773902, whole genome shotgun sequence.
ACCESSION   DAOSIF010000148 DAOSIF010000000
VERSION     DAOSIF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12418_length_5818_cov_4.773902"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLT35_06395"
     CDS             complement(<1..545)
                     /locus_tag="PLT35_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG28896.1"
                     /translation="MVTTNVREGRIVQIIGPVIDVEFEGGHLPPVYNALEIAAEIDGK
                     AAVVVAEVEQHLGEGRVRAVSMKPTDGLQRGMTAVDLGGPISMPVGPATLGRVLNVLG
                     EPVDFADKPVNAAERWPIHRPAPTLVEQSTEARMFETGIKVVDLLEPYLQGGKIGLFG
                     GAGVGKTVIIQELIHNIALKHG"
     gene            complement(683..1543)
                     /gene="atpG"
                     /locus_tag="PLT35_06400"
     CDS             complement(683..1543)
                     /gene="atpG"
                     /locus_tag="PLT35_06400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOG28897.1"
                     /translation="MPSLIDLRRRIRAVKSTQQITKAMKMIAASRLRKSQERIVNARP
                     YANETLRVLQNLASRTDPDAHPLLAVREERAALLVIITADKGLCGSFNTNIIKTAGQE
                     ILAGGGRRVALALIGRKAREFFGRRGFPIVYERSHFFQHLAFDDAAAIAAVAIEEYCE
                     GRADAVYLVYNEFKSVLQQRVVTEKLLPIERRSVDEAGPSARVDYLYEPDAATLFGQL
                     LPAYVRTEVWRALLESAAAENAARMTAMDAATKNATEMIDGLTLYMNKVRQAGITNEI
                     IEVVSGAEAL"
     gene            complement(1543..3072)
                     /gene="atpA"
                     /locus_tag="PLT35_06405"
     CDS             complement(1543..3072)
                     /gene="atpA"
                     /locus_tag="PLT35_06405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG28898.1"
                     /translation="MTIKAEEITKIIRDQIGSHAVDVDVSEVGSVVSVGDGIARVHGV
                     DSTMSGEMLEFPHGVFGIALNLEEDSVGCVLLGEHHAIAEGDPVRRSGRIISVPVGDE
                     MVGRVVNSLGQPVDGKGPIVTSRYAPIERIAPGVVDRKGVKEPLQTGLKAIDSMVPIG
                     RGQRELVIGDRQTGKTAIVVDTIINQKGQDVVCIYNAIGQKQSTIAQVVKTLEDAGAM
                     EYSIVVAAAASDPAPMLYISPYAACTMGEFFRDTGRHALCVYDDLSKHAQAYREISLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLREDLGGGSLTALPIIETQAGDLSAYIPTNV
                     ISITDGQIFLETDLFHQGVRPAINVGNSVSRVGGSAQVKAMRQVAGTLRLDMAQFREL
                     AAFAQFGSDLDKATQAQLNRGRRLVEVLKQPQYRPLPVEKQVLMIFAGTNGFLDPVAV
                     DEVGKYEEEMYRFFDSHKPSLLRTLAERGQFDDGLKAEIASALEEFAEVFAAAKRTVA
                     A"
     gene            complement(3181..3729)
                     /gene="atpH"
                     /locus_tag="PLT35_06410"
     CDS             complement(3181..3729)
                     /gene="atpH"
                     /locus_tag="PLT35_06410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOG28899.1"
                     /translation="MTARASAKRYARALLDVALAEADASAVERQLARVSGLFTGHADL
                     WAVMTNPAVPAPKKRAVVEAMLPRLEVAPVVAKLLQLLAGRDRLALLPDIVEAYGNRL
                     LDHRRVVRAVVTSAVPLEAERTKALERELAGITGRTVIVSAETDPAIVGGVVARIGST
                     VYDGSVRRQLEKIRERLAAAGQ"
     gene            complement(3726..4424)
                     /locus_tag="PLT35_06415"
     CDS             complement(3726..4424)
                     /locus_tag="PLT35_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28900.1"
                     /translation="MRHGVTLVLAAAAAALILAPSVAPAQQERAAAAASGAAAHRAAE
                     GVAGEEPPEGTDGHAGGGDHGSPVVDLVARLFNFGILAGTLVYFLRSPLSGYLSSRAA
                     GIRSGLANAAQTRRAAEADLVSIEARMAGLPAELEAIRKAGAAEVEAEETRIREAAAR
                     ERVRLLDQARREIDWQVKIAQRDLTAHAAELAVGVAARRLTATITAEDQARLFDRYLS
                     QLGRAAEPGRQVRA"
     gene            complement(4449..4871)
                     /locus_tag="PLT35_06420"
     CDS             complement(4449..4871)
                     /locus_tag="PLT35_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28901.1"
                     /translation="MLPNLSVVFVVVAVVLLTAVLERVLFRPLLRVMREREAALRSAT
                     DLAEAAAARARSAAAEFDAKVTAARAELYKQMDERRKAAEGYRNELVADTRAEVDAQL
                     ASAKAELAAQAARARATLEAEADALGGDIAAKVLGRPS"
     gene            complement(4988..>5818)
                     /gene="selD"
                     /locus_tag="PLT35_06425"
     CDS             complement(4988..>5818)
                     /gene="selD"
                     /locus_tag="PLT35_06425"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOG28902.1"
                     /translation="FGRIAATNALSDVYAMGGRPRTALAIAAFPQKDFDPALIRAIFT
                     GGFDKLREAGVALLGGHTVRDNEIKFGYAVTGEVDRGRVLSNAGARAGDVLFLTKPIG
                     TGIVGTAIKAGCAPAAAVQAAVASMTTLNRAAAEALQRLPAGLVHACTDITGFGLVGH
                     ASELADASAVTLEIDADCVPLLPGVADLAAGNRPGGLASNLEFFERGVAVGGGVDESL
                     LWLLYDPQTSGGLLICAAGEHAEAVRRALSEAEIAAAEIGRAVDRGAETVRVGRRARR
                     "
BASE COUNT          779 a         2210 c         1933 g          896 t
ORIGIN      
        1 ccgtgcttga gggcgatgtt gtggatgagc tcctggatga tgacggtctt gccgacgccg
       61 gccccgccga acagcccgat cttcccgccc tggaggtacg gctcgagcag gtcgacgacc
      121 ttgatgccgg tctcgaacat cctggcctcg gtcgactgct cgaccagcgt cggcgccggg
      181 cggtggatcg gccagcgctc cgcggcgttc accggcttgt cggcgaagtc caccggctcg
      241 cccagcacgt tcagcacccg gcccagcgtc gccgggccga cgggcatgct gatcgggccg
      301 cccaggtcca cggcggtcat gccgcgctgc aggccgtcgg tgggcttcat cgacacggcg
      361 cggacgcgcc cctcgccgag gtgctgctcg acttccgcca cgaccacggc ggccttgccg
      421 tcgatctcgg ccgcgatctc gagggcgttg tacaccgggg gcaggtggcc gccctcgaac
      481 tccacgtcga tgacgggccc gatgatctgg acgatgcggc cctcacgaac attagtagta
      541 accattggca ttcgtccccg tcattcctgg gggctagggg ctgggcgcta ggcgcttgtc
      601 accagcgccg ccacaccgcg attccgtcca cggggcgatg ccccgtcgga ctagccccca
      661 gcgccaagcc ccgagcgcct ggctacagcg cctccgcccc cgacacgacc tcgatgattt
      721 cgttcgtaat ccccgcctgg cgcaccttgt tcatgtacag cgtcaggccg tcgatcatct
      781 ccgtcgcgtt cttcgtcgcg gcgtccatcg ccgtcatgcg cgccgcgttt tcggcggccg
      841 ccgactcgag cagcgcccgc cacacctccg tccgcacgta cgccggcagc agctgcccga
      901 acagcgtcgc cgcgtccggc tcgtacaggt aatccacccg ggccgagggg ccagcctcgt
      961 ccacgctccg ccgctcgatg ggcagcagct tctcggtcac cacgcgctgc tggagcacgg
     1021 acttgaactc gttgtacacg aggtacaccg cgtccgcgcg cccctcgcag tactcctcga
     1081 tggccaccgc cgcaatcgcc gccgcgtcgt cgaaggcgag gtgctggaag aagtgcgagc
     1141 gctcgtacac gatcgggaac ccgcggcggc cgaagaactc gcgcgccttg cgcccgatga
     1201 gcgcgagcgc gacgcggcgg ccgccgccgg ccaggatctc ctggccggcg gtcttgatga
     1261 tgttcgtgtt gaagctgccg cacaggccct tgtcggccgt gatgatcacg agcagggcgg
     1321 cgcgctcctc gcggaccgcc aggagcgggt gcgcgtccgg gtcggtccgc gaggccaggt
     1381 tctgcagcac gcgcagcgtc tcgttggcgt agggccgcgc gttgacgatc cgctcctgcg
     1441 acttgcggag cctcgacgcg gcgatcatct tcatcgcctt cgtgatctgc tgggtcgatt
     1501 tcaccgcccg gatgcgccgc cggagatcga tgagcgaggg cattacgccg ccaccgtccg
     1561 cttcgcggcg gcgaacacct ccgcgaactc ctcgagcgcc gacgcgatct ccgccttcag
     1621 cccgtcgtcg aactgccccc gctcggccag cgtccgcagc agcgagggct tgtgcgagtc
     1681 gaagaagcgg tacatctcct cctcgtactt cccgacctcg tcgacggcga cgggatcgag
     1741 gaagccgttc gtgccggcga agatcatcag gacctgcttc tccaccggga gcgggcggta
     1801 ctgcggctgc ttcaggacct ccaccaggcg cctgccccgg ttgagctggg cctgcgtggc
     1861 cttgtcgaga tcgctgccga actgcgcgaa cgccgccagc tcgcggaact gcgccatgtc
     1921 gaggcggagc gtgccggcca cctgccgcat ggccttgacc tgcgcggagc cgcccacccg
     1981 cgacacggag ttgccgacgt tgatcgccgg gcggacgccc tggtggaaca agtcggtctc
     2041 caggaagatc tgcccgtcgg tgatcgagat cacgttggtc gggatgtacg ccgagaggtc
     2101 ccccgcctgc gtctcgatga tcggcagcgc cgtcagcgag ccgccgccga ggtcctcgcg
     2161 cagcttcgcc gcgcgctcga gcagccgcga gtgcaggtag aagacgtcgc ccgggtacgc
     2221 ctcgcgcccc ggcgggcggc gcagcagcag cgagatctcg cggtacgcct gcgcgtgctt
     2281 cgacaggtcg tcgtagacgc agagggcgtg gcggccggtg tcccggaaga actcgcccat
     2341 cgtgcacgcg gcgtacgggc tgatgtagag catcggggcc ggatcggagg ccgccgccgc
     2401 gacgacgatc gagtactcca tcgcccccgc gtcctcgagc gtcttcacga cctgcgcgat
     2461 ggtcgactgc ttctgcccga tggcgttgta gatgcagacg acgtcctgcc ctttctggtt
     2521 gatgatcgtg tcgaccacaa tcgccgtctt gcccgtctgg cggtcgccga tgacgagctc
     2581 gcgttggccg cggccaatcg gcaccatcga gtcgatggcc ttcagccccg tctggagcgg
     2641 ctccttcacg cccttccgat cgacgacgcc cggcgcgatg cgctcgatgg gcgcgtagcg
     2701 gctcgtgaca atcggcccct tgccgtccac cggctggccg agagagttca ccacgcgccc
     2761 gaccatctcg tcgccgacgg gcaccgagat gatgcggccc gagcggcgca ccgggtcgcc
     2821 ctccgcaatg gcgtggtgct cgcccaggag cacgcagccc acgctgtcct cctcgaggtt
     2881 cagggcaatg ccgaacacgc cgtgcgggaa ctcgagcatc tcgcccgaca tggtcgagtc
     2941 cacgccgtgc acgcgcgcga tgccgtcgcc cacggacacc acggacccga cttccgacac
     3001 gtccacgtcc acggcatggc tgccgatctg gtcgcggatg atcttggtga tttcctcggc
     3061 tttgatggtc atggcctcgt tcgtttctcg ccgccgccgt cgttcgccgc aggaggcgcg
     3121 cccgccgccg ctggcgccgc gtcttgcgcg ttccgcgttc cgactccccc gccccggctc
     3181 ctactgcccc gccgccgcca gccgctcgcg gatcttctcg agctgccgcc gcacgctccc
     3241 gtcgtacacc gtgctcccga tgcgcgcgac gacgccgccg acgatcgcgg gatccgtctc
     3301 ggccgacacg atgaccgtgc gcccggtgat gccggccagc tcccgctcga gcgccttcgt
     3361 ccgctcggcc tccaggggca ccgccgacgt gacaacagcg cgcaccaccc gccggtgatc
     3421 gagcaggcgg ttcccgtagg cctcgacgat gtccggcagc agcgcgagcc gatcgcggcc
     3481 ggccagcagc tgcagcagct tcgccaccac gggcgccacc tcgagcctcg gcagcatcgc
     3541 ctccaccacc gcccgcttct tcggcgccgg caccgcgggg ttcgtcatca ccgcccagag
     3601 atccgcgtgg cccgtgaaga ggcccgacac ccgggcgagc tgacgctcca cggccgaagc
     3661 gtccgcttcg gccagggcga cgtccagcag cgcccgcgca taccgcttgg cgctcgcccg
     3721 ggcggtcatg cgcgcacctg ccgtccgggt tcggccgccc ggccgagctg gctcaggtag
     3781 cggtcgaaca ggcgggcctg gtcctcggcc gtgatcgtcg ccgtcagccg cctggccgcc
     3841 acgcccacgg ccagctcggc ggcgtgcgcc gtgaggtcgc gctgcgcgat cttcacctgc
     3901 cagtcgatct cgcgcctggc ctgatccagc agccggacgc gctcgcgggc cgccgcctcc
     3961 cggatgcgcg tctcctcggc ttccacctcg gcggcgcccg ccttgcggat ggcctccagc
     4021 tcggcgggca gcccggccat cctcgcctcg atggacacga gatcggcctc ggccgcgcgc
     4081 cgcgtctgcg ccgcgttcgc caggccgctc ctgatgcccg ccgcgcggct gctcaggtat
     4141 cccgagagcg gcgaccgcag gaagtacacc agcgtgcccg cgaggatgcc gaagttgaac
     4201 agcctcgcga ccaggtccac gacgggactg ccgtgatcgc cgccgccggc gtgcccgtcc
     4261 gtgccctcgg ggggctcctc gcccgcaacg ccctcggccg ctcgatgcgc ggcagccccg
     4321 gacgcggcgg ccgccgcgcg ctcctgctgc gccggcgcca cgctcggcgc aaggatcagg
     4381 gcggccgccg ctgcggccag aaccagagtg acgccgtgtc gcatgaggga tgtcctcgac
     4441 cgtcggggct aactggggcg tccgagcact ttcgcggcga tgtcgccgcc aagggcgtca
     4501 gcctcggcct cgagcgtggc gcgtgcccgg gcggcctggg ccgcgagctc ggctttcgcg
     4561 gaagccagct gcgcgtccac ttccgcgcgc gtgtccgcga cgagctcgtt ccggtatccc
     4621 tcggccgcct tccgccgctc gtccatctgc ttgtacagct ccgcgcgcgc cgccgtcacc
     4681 ttcgcgtcga actcggccgc cgccgacctg gccctggccg ccgccgcttc cgcaagatcc
     4741 gtcgccgacc ggagggccgc ctcgcgctcg cgcatcaccc gcagcaacgg ccggaacagc
     4801 acgcgttcca ggacggccgt gagcagcacc acggcgacga ccacgaacac gaccgacagg
     4861 ttcggaagca cggcgtcgac ctcgcgtccg caccctgcgc ggacgtcaaa acagagattc
     4921 tatacgggag acaccacgcg gggctagtcc tgagggggct cgcggctcgg gatttctcta
     4981 tctcgcttca cctcctcgct ctcctaccca ccctcaccgt ctccgctccc ctgtccaccg
     5041 ctctcccaat ctccgcggcg gcgatttccg cctccgacaa ggcgcgccgg acggcctcgg
     5101 cgtgctcgcc ggccgcgcag atgagcaggc cgccggaggt ctgcggatcg tagagcagcc
     5161 acaggaggct ctcgtcgacg ccgccgccga ccgccacgcc gcgttcgaag aattccaggt
     5221 tgctcgccag cccgcccggg cggttgcccg ctgccaggtc cgcgacgcca ggcagcaggg
     5281 gcacgcagtc ggcgtcgatc tcgagcgtga cggcgcttgc atcggccagc tcgcttgcgt
     5341 ggccgaccag gccgaacccc gtgatgtccg tgcacgcgtg cacgaggccc gcgggcagcc
     5401 tctgcagcgc ctccgccgcg gcgcggttga gcgtcgtcat cgacgccacc gcggcctgga
     5461 ccgcggctgc cggcgcgcac ccggctttga tggccgttcc gacgatgccc gtgccgatgg
     5521 gcttggtcag aaagaggacg tcgccggccc tggcgcccgc gttcgagagc acgcggccgc
     5581 ggtcgacctc tcccgtcacg gcgtagccaa acttgatttc gttgtcacgc acggtgtggc
     5641 cgccgagcag ggccacgccg gcctcgcgca gcttgtcgaa gccgccggtg aagatcgcgc
     5701 ggatgagtgc cggatcgaag tccttctgcg ggaaggcggc gatggcgagc gccgtccgcg
     5761 ggcggccgcc catggcgtac acgtcgctca gggcgttggt ggcggcgatg cggccaaa
//
LOCUS       DAOSIF010000149         5813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12433_length_5813_cov_3.105940, whole genome shotgun sequence.
ACCESSION   DAOSIF010000149 DAOSIF010000000
VERSION     DAOSIF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12433_length_5813_cov_3.105940"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="PLT35_06430"
     CDS             <1..1776
                     /locus_tag="PLT35_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOG28903.1"
                     /translation="PAAAPGAGQAGRHAFTLGPHDFLLDGRPFQIRGCEIHPARVPAE
                     YWAHRIRMAKALGCNTISAYLFWNYHETREGAFDFKTGNRDIARFIALAQAEGMWVLL
                     RPGPYVCAEWDFGGLPPYLLRDPELKVRCLHPTYMKAVERFIATLVPLVRPLLVTRGG
                     PILMVQVENEYGSYGNDRAYMARLKDLFVSAGIDVPLYTADGPTPHMLEAGHLPGAAV
                     GLDSGTTEDHWTLARSIVPGVPVFSAETYPGWLTHWGEAWARPSPDALLKEVAFLLSN
                     RKSFSLYVAHGGTNFGFTAGANSGGAGYEPDVTSYDYDAPIGEQGRPAPKYYALREAI
                     AASLPAGEKLPPVPDPIPAVAIPEFHMRAYASIWENLPAPQRSVQPRPFEAYGQNQGL
                     VLYRTALVGRKAGRLVVTDLHDYATVFLNGAFVGTLDRRLGQKSIELPKTDAAMPVLD
                     ILVEGMGHINFAQEMIDRKGITERVTLAGMTLMNWEIFLLPLDDAWVEALPEGAVDRV
                     RPGTFYKGSFNLDRPADTYLDMTGYRKGVVWVNGHNLGRYWDIGPQRRLYCPAPWLKA
                     GANDVVVFDLLAALPAPLRGMPELE"
     gene            1783..3066
                     /locus_tag="PLT35_06435"
     CDS             1783..3066
                     /locus_tag="PLT35_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG28904.1"
                     /translation="MVTGGRGGRADWGLVALLSGTATAAYLCRVNVSVAGALIMEEYG
                     LNQAQMGRVFSAFLLGYTLLMMPGGLLADAWGTTRTLSRLAWGWVALTALHAALPRAD
                     RGADVLVVLAGLVALRFLMGAFAAPTYPAAVQGVSRWIDPRAHGRANGFIIGSVGLGS
                     AIAPPFVTAVMIRWGWRAAMVASALPALALAAVWLIVREPADARPAGRPPQPAGGPPE
                     ARGAADARSVARLLRVPGFGWLTLSYSLQGYVGYIFIFWFYLYLVQVRHISLVEGAVW
                     SSLPWVLSTVSIPAGGWVSDRLVQTRLGPAWGRRLVPMVGMGGAGLFISIGAHTATAW
                     LAAGSLAVATALVLSVEGPFWTTAARLARARSATGGAVMNTGCNIGGLVSPMLTPVLG
                     QAFGWEPALHVAAMLSVAGALAWLRINPAGPPAAR"
     gene            complement(3079..3993)
                     /locus_tag="PLT35_06440"
     CDS             complement(3079..3993)
                     /locus_tag="PLT35_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOG28905.1"
                     /translation="MPRRQAAGVLARMSAEVASLAARVLRATVAVAGPTPDGGSSGSG
                     FFIDAGGHIVTNQHVVDDVTPPITISLHGGGTALAGVVGVDPIADIAVLRLNGEWPHH
                     LSLRRSRVRPGEMCLAVGNPLGRYPESVTIGVVSGLARTAAAGTGRPHYDLLQTDCDI
                     HEGNSGGPLVDVRGRVIGITTLLDTESPHIGLAVPAETVRVIVPELLANGRVDRATLG
                     VSVARRTVTVEGKPVAGLEVVRITRPKDQRLEAGDVLVSLAGRPVRDAPALFALLGRE
                     RIGRATPAEVIRRGRRVAVSVIPWRLES"
     gene            complement(3986..4426)
                     /locus_tag="PLT35_06445"
     CDS             complement(3986..4426)
                     /locus_tag="PLT35_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28906.1"
                     /translation="MSRIDVLRPFIETNLAALLETDRVAPDEDGEYSFPRGSAEITLR
                     LLDAPMPLLQFSAVLVSGLKKKVRVLEALNEVNAAELGFRVFRFEDLVIAAWEIPADT
                     LDARQFADACRRFAAAADRFDSIIARKFGGRTARSDADEEAVDA"
     gene            4647..>5813
                     /locus_tag="PLT35_06450"
     CDS             4647..>5813
                     /locus_tag="PLT35_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOG28907.1"
                     /translation="MQLQAIDWIIVALSLLICFVPALFFGKRAGKSTSEFFASGRAVP
                     WWLAGLSMVATTFSSDTPNLVTDIVRRNGVAGNWVWWAFVLTGVATVFFYARLWRRSG
                     VMTDLEFYQVRYSGKAASVVRGFRAVYLGFFFNCMIMATVNLAACKIAGVLFGFERWQ
                     TLAFVGALNVVFATHSGLWGVLVIDMIQFFIKMTAVIAAAYFAVKLPQVGGLDGLITK
                     LSATSGPNGINYLNILPDFANHWEIAVAVFIMPIAVQWWAVWYPGAEPGGGSYIAQRM
                     LASKSERDALGAVLFFNVAHYVLRPWPWIITALASIIVYPQLGDIQAAFPHLDPTLIG
                     HDTAFPAMLKFLPIGFMGLMVGGLIAANSSTILTHLNWGASYLVHDFWRQFIRKG"
BASE COUNT          783 a         2022 c         2067 g          941 t
ORIGIN      
        1 cccgccgccg ctccaggcgc cggccaggcg ggccgtcacg ctttcacgct cgggccgcac
       61 gacttcctgc tcgacgggcg gcccttccag atccgggggt gcgaaatcca cccggcgagg
      121 gtgcccgcgg aatactgggc gcaccggatc cggatggcga aggccctcgg ctgcaacacc
      181 atctcggcct acctcttctg gaactaccac gaaaccaggg aaggcgcctt cgacttcaag
      241 acgggcaacc gggacatcgc gcgcttcatc gcgctcgcgc aggccgaggg catgtgggtc
      301 ctgttgaggc cagggcccta cgtctgtgcc gagtgggact tcgggggcct cccgccttac
      361 ctgctccgcg atccggagct gaaggtccgc tgccttcacc ccacgtacat gaaggcggtg
      421 gagcgtttca tcgcgaccct cgtcccgctg gttcgccctc tcctcgtcac gcggggcggc
      481 ccgattctga tggtccaggt ggagaacgag tacggaagtt acgggaacga ccgggcctat
      541 atggccaggc tgaaggacct cttcgtcagc gccgggatcg acgtgccgct ctacaccgcc
      601 gacggtccca cgccgcacat gctcgaggcg gggcacttac cgggcgcggc cgtaggcctc
      661 gactcgggaa cgaccgagga ccactggacg ctggcgcgct cgattgtgcc gggcgtgccg
      721 gtcttctccg ccgagaccta ccccgggtgg ctcacgcact ggggcgaggc ctgggcgcgg
      781 cccagcccgg acgcgctcct caaggaggtc gccttcctgc tctcgaaccg gaagtccttc
      841 agcctctacg tggcccacgg cggcacgaac ttcggcttca cggcgggggc gaactcgggc
      901 ggcgcaggct acgaacccga cgtgaccagc tacgactacg acgcgcccat cggcgagcag
      961 ggccggccgg ctccgaagta ctacgccctg cgcgaggcca ttgcggcgtc gctgccggcg
     1021 ggcgagaagc tgccgccggt tcctgacccc atcccagccg tggcgattcc ggagttccac
     1081 atgcgggcct acgcctccat ctgggaaaac ctgccggcgc cgcagcgctc ggtccagccc
     1141 cgcccgttcg aggcgtacgg ccagaaccag ggcctcgtcc tctatcgcac cgcgctcgtc
     1201 gggaggaagg cgggccggct ggtggtcacc gacctgcacg attacgcgac ggtctttctg
     1261 aacggcgcgt tcgtcggcac gctggatcgc aggctcggcc agaagtcgat tgagctcccg
     1321 aagacggatg ccgcgatgcc ggtgctcgat atccttgtcg aagggatggg ccacatcaac
     1381 ttcgcgcagg agatgatcga ccgcaagggc atcaccgagc gagtcaccct cgccggcatg
     1441 acgctcatga actgggagat cttcctcctg cccctcgacg acgcctgggt ggaggcgctg
     1501 ccggaggggg cggtggaccg cgtccgccca ggaacgttct acaagggctc cttcaacctc
     1561 gatcggcccg ccgacaccta tctggacatg acgggctatc ggaagggcgt ggtctgggtc
     1621 aacgggcaca acctcggtcg ctactgggat atcgggcccc agcgacggct gtactgcccg
     1681 gcgccgtggc tcaaggccgg cgccaacgac gtggtcgtgt tcgatctcct cgccgcgtta
     1741 ccggctcccc tgcgcggcat gccggagctg gagtaggcgg gcgtggtcac cggcggccgc
     1801 gggggacggg ccgactgggg cctggtcgcg ctcctctcgg gaaccgcgac ggcggcgtac
     1861 ctttgccgcg tgaacgtctc ggtggccggc gcgctcatca tggaggagta cggcctgaac
     1921 caggcgcaga tgggccgcgt cttcagcgcg ttcctgctcg gctacacgct gctgatgatg
     1981 cccgggggcc tgctggccga cgcgtgggga accacgcgca cgctgagccg gctcgcgtgg
     2041 ggatgggtcg cgctgaccgc cctgcacgcc gcactgccgc gggccgaccg gggcgccgat
     2101 gtcctggtgg tgctggccgg gctggtcgcc ctgcggttcc tgatgggcgc cttcgccgcg
     2161 ccgacctacc cggctgccgt ccagggcgtc tcccgctgga tcgatccgcg cgcgcacggg
     2221 cgggcgaacg gcttcatcat cggctccgtc ggcctcggct cggcgatcgc gccgcccttc
     2281 gtgacggcgg tgatgattcg ctggggatgg cgggcggcga tggtggcgtc agccctgcca
     2341 gcgctcgccc tggccgcggt gtggctgatc gtccgcgagc ctgccgacgc gcgcccggcc
     2401 gggcggccgc cgcaaccggc aggaggcccg cccgaagccc gcggcgcggc cgacgcgcgg
     2461 tctgtcgcgc gcctcctccg ggtcccgggc ttcggctggc tcaccttgag ctactcgctc
     2521 cagggctacg tgggctacat tttcatcttc tggttctacc tctacctggt ccaggtccgc
     2581 cacatcagcc tcgtcgaggg agcggtctgg agcagcctgc cgtgggtcct gtccaccgtg
     2641 tccatccccg cgggaggatg ggtgtcggat cggctggttc agacccgcct gggcccggcc
     2701 tggggccgcc gtctggtgcc gatggtcggc atgggaggcg ccggcctctt catctccatc
     2761 ggggcgcaca cagcaaccgc ctggctggca gcgggctccc tcgccgtggc aacggccctc
     2821 gtgttaagcg tcgaagggcc gttctggacg acggcggccc gccttgccag ggcgcgaagc
     2881 gccaccggcg gagccgtcat gaacaccggc tgcaatatcg gaggcctcgt gtctcccatg
     2941 ctgacgccgg tgctcgggca ggcgttcggc tgggagccgg cgctgcacgt ggcggcgatg
     3001 ctgtcggtgg cgggggcgct ggcgtggctg cggatcaacc cagccggccc gcccgcggct
     3061 cggtgacggt ggcggccgtc aggattcgag gcgccagggg atgacgctca cggccacccg
     3121 gcgccctcgc cggatgactt ccgccggcgt ggcgcggccg atccgctcgc ggccgaggag
     3181 cgcgaacagg gcgggcgcgt cgcgcacggg ccgcccggcg aggctcacca gcacgtctcc
     3241 cgcctcaagg cgctggtcct tcgggcgtgt gatccgcacg acttcgagcc ccgcgaccgg
     3301 cttcccctcc acggtcaccg tccgcctggc gaccgacacg ccgagcgtgg cgcggtccac
     3361 gcgcccgttg gcgagcaact ccggcacgat gacgcggacg gtctcggcgg gcacggcgag
     3421 gccgatgtgc ggcgactcgg tgtcgagcag ggtcgtgatg ccgatcacgc ggccccgaac
     3481 gtccacgagc ggtccgccgg agttgccttc gtggatatcg cagtcggtct gcaggagatc
     3541 gtagtggggc ctgccggttc cggcggcggc cgtgcgggcg agcccgctga cgaccccaat
     3601 cgtcactgac tccgggtatc ggcccagcgg gttgccgacg gcgaggcaca tctcgcccgg
     3661 gcgcactctg gagcggcgca gtgacagatg gtgcggccac tccccgttca ggcggagcac
     3721 ggcgatgtcc gcgatcgggt cgacgccgac cacgccagcg agcgcggtgc cgccgccgtg
     3781 caggctgatg gtgatgggcg gtgtgacgtc gtcgacgacg tgctggttgg tgacgatgtg
     3841 gccgccggcg tcgatgaaga agcccgagcc gctgctgccg ccgtccggcg tcgggccggc
     3901 caccgcgacg gtggcccgca gcacccgcgc ggccaggctg gcgacctcgg cgctcatacg
     3961 ggccaggacg ccggctgcct gccgcctagg catcgacggc ctcctcgtcg gcgtcagagc
     4021 gcgccgtccg cccgccgaac ttgcgcgcga tgatcgagtc gaaccggtcg gcggcggcgg
     4081 cgaaccgccg gcatgcgtcg gcgaactgcc gggcgtcgag ggtgtcggcg ggtatctccc
     4141 aggcggcgat gaccaggtcc tcgaagcgga agacgcggaa cccgagttcc gcggcgttca
     4201 cctcgttcag cgcctcgagc acccgcacct tcttcttcag gcctgagacg aggacggccg
     4261 agaactgcag gagaggcatc ggagcgtcga ggaggcggag cgtgatttcg gcgctgccgc
     4321 ggggaaagct gtactcgccg tcctcgtctg gagccacgcg gtccgtctcg agcagcgccg
     4381 cgaggttcgt ctcgatgaag gggcgaagga cgtcgatccg cgacatgctg cctccgtggt
     4441 cgggacgccg gccgcgcggc ggcggccgcc cggcggccgg ttccggtcgg tgggaacagt
     4501 ctacgccagc cgccgcccca ggggtgacac aaccggggcg gcagggccgt cggcggcgcg
     4561 gcgcgtttcg gagggcgccg tctgtgttat cgtgtcgtgg ctcatcgggc gcccgagacg
     4621 ggcctccagg acggggacag gaccttatgc agcttcaggc catcgactgg attatcgtcg
     4681 cgctctcgct ccttatctgc ttcgtgccgg cgctgttctt cgggaaacgc gccggcaaga
     4741 gcacctccga gttcttcgcc tcggggcgcg ccgtgccctg gtggctggcc gggttgtcga
     4801 tggtggccac caccttcagc agcgacaccc cgaacctggt gaccgacatc gtccgccgca
     4861 acggcgtggc cggcaactgg gtgtggtggg cgttcgtgct gacgggggtc gccaccgtgt
     4921 tcttctacgc ccggctctgg cgccgatcgg gcgtgatgac ggacctggag ttctaccagg
     4981 tccgctactc ggggaaggcc gccagcgtcg tgcgcgggtt ccgcgcggtg tacctgggct
     5041 ttttcttcaa ctgcatgatc atggcgacgg tcaacctggc ggcgtgcaag atcgcgggcg
     5101 tgctgttcgg gttcgagcgc tggcagacgc tggcgttcgt gggggcgctg aacgtcgtct
     5161 tcgccacgca ctccggcctc tggggcgtgc tcgtcatcga catgatccag ttcttcatca
     5221 agatgacggc cgtgatcgcg gcggcgtatt tcgccgtgaa gctgccccag gtcggcggcc
     5281 tcgacgggct gatcacgaag ctgtcggcca cgtcgggccc caacgggatc aactacctga
     5341 acatcctgcc ggatttcgcc aaccactggg agatcgcggt cgccgtgttc atcatgccga
     5401 tcgcggtgca gtggtgggcc gtctggtatc cgggcgcgga gccaggcggc ggcagctaca
     5461 tcgcgcagcg catgctggcg tcgaagtcgg aacgggacgc gctgggcgcg gtgctcttct
     5521 tcaacgtggc ccactacgtc ctgcggccgt ggccgtggat catcacggcg ttggcgtcca
     5581 tcatcgtcta cccgcagctc ggcgacatcc aggcggcctt cccgcacctc gacccgacgc
     5641 tcatcggcca cgacacggcc tttcccgcga tgctgaagtt cctgcccatc ggattcatgg
     5701 gcctcatggt gggcggcctc atcgcggcga actcctcgac gatcctcacg cacctcaact
     5761 ggggcgcgtc gtacctggtc cacgacttct ggcgccagtt catccgcaag ggc
//
LOCUS       DAOSIF010000150         5810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12438_length_5810_cov_5.007646, whole genome shotgun sequence.
ACCESSION   DAOSIF010000150 DAOSIF010000000
VERSION     DAOSIF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12438_length_5810_cov_5.007646"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLT35_06455"
     CDS             complement(<1..367)
                     /locus_tag="PLT35_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28908.1"
                     /translation="MPRLTILADENVPFAREAFGTLGDVRLEHGRRILPAGLADVDLL
                     VVRSITRVDAALLAATPVRFVGTATSGTDHVAEPDLERLGIAFYAARGCNAAAVAEYM
                     AAAWLTLAARRGETLAGRRV"
     gene            complement(468..3440)
                     /locus_tag="PLT35_06460"
     CDS             complement(468..3440)
                     /locus_tag="PLT35_06460"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOG28909.1"
                     /translation="MERTGWERIDELFEAALQQPAGERETFLLGATDDERLRAEVRAL
                     LHEHDSDPWFVDEPSAAPIGPPRAAAAPSPAPPRLPVVAAADEPPAALVQRAASRPQP
                     GSAARPGGPAARPGGPAPTWPTARPLPPARGEPHAAPSAAPSAAAPSAAAPSAATPSA
                     AAPSAASLQPQAPRAHALPRPPSIQPQSPRPTPPASPPAASALRRTPVLREPPDGQPE
                     TAPELAPGALFGAYRIIRQVGRGGMGAVYEAADQSGASPQQVTLRVLPPGWNTAAWTD
                     WFRAACHLVAGLDHPWVARLLDGGVAEGGMPFVAMEHVAGEDICAYCRSRETSLPDRV
                     RLVLRVCTALEYAHQRLIVHRSVGPKNILVTADGQLKVMNCGVARLLFGRPDSGEGLT
                     RTGQSLFTPEYVSPEQARGELITAASDIYSLGVLLYVLLTDHPPYEFRDLTPVEMTRV
                     ICDTDPEPPSRVAPPSQRRALANDLDAVVMKALRKDPRMRYQSVTAFADDLRAWLHGR
                     TVSVFPASRRQRAAKALRRHWLLAAVLGAAIAALGAGTALFGWQARALRAERDLAGGR
                     LRVSQQFSRSLLNELHEAIAALSASAPARTAVLTRAVEQVDGLARDAAGDPAAALDLA
                     RAYRLVAALQAATSGGAPAGRASAAASLDAAVAAGERALAAEPESVEAAIALIAACGD
                     AAALRQELGDDEAAGRADARHLGLVERLARQQPRDALARAAASSGYGRLGAFRAARGD
                     LAGARMMYAYAAAGFETLAAENVLPAAARRDYAGVLRALGGFLLADGALDDADARFAS
                     AQLLEREDLAGQPGDESARRGLAASTGGLADVAMRRGDHAKAESLWLQALADAQASED
                     GGADDAAAAEIAAAARASLAELCRSQRRLDEALRHAREYARIRERLADAPGAPAAAAL
                     DLAVARTAVARVQLDVLDARPSSPDEGWRLRDAGALLTRAFPPIRAAAPSSAQQAALA
                     EIERQTARFSRLTAARQP"
     gene            3563..4348
                     /locus_tag="PLT35_06465"
     CDS             3563..4348
                     /locus_tag="PLT35_06465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOG28910.1"
                     /translation="MRRTGSRADGPPHGAQLRLPFGRSARETTRGAVVSVPGVGAGVV
                     FVRHRRARQYVLRLRDDGSVRVTLPWRGSRAEAQRFVRERRDWILRQRLQHAASAAAR
                     SGPWLPGTSVLLRGVEEPIRVQPAGGALAVTLGSERLVLPASACHNLRPPLEAHLRSV
                     AARELRGRLHELARAHGFTVAAVSIRAQRTRWGSCSRSGRISLNWRLIQVPASVRDYV
                     LLHELAHLKHADHSARFHREVERLCPHCAEARRWLRETTLLGR"
     gene            4444..4950
                     /locus_tag="PLT35_06470"
     CDS             4444..4950
                     /locus_tag="PLT35_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28911.1"
                     /translation="MTGMDSLFSEGTRRGARGALVAAALVVAALVVAASAVPALAQDG
                     PGLRAGLSASPDQIFFGGHYVTQPLTGMLRFQPNLEVGLGGGQTTIAFNGEFALWRKI
                     SPDWHVYLGGGPSILAVTHQHRDDTDIDPGFNLVAGLRRRGGAFFELKIGRIDSPSFK
                     ALVGFTIR"
     gene            complement(5008..>5810)
                     /gene="meaB"
                     /locus_tag="PLT35_06475"
     CDS             complement(5008..>5810)
                     /gene="meaB"
                     /locus_tag="PLT35_06475"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOG28912.1"
                     /translation="LGLRLLAQGRRVAVLAVDPSSSVSGGSILGDKTRMERLSADERA
                     FIRPSPSSGTLGGVADKTREAMLAVEAAGHDVVIIETVGVGQSETAVAGMTDMFVLLQ
                     LPNAGDDLQAIKKGITELADLIAINKADLDEAAATNARAQITSSLRILGLHGRGEEEP
                     LDAAAWHPEVLTVSALTGQGLDAFEDAISRFREMQQASGRFALRRRQQAEAWMKERVE
                     AGLRERFRAHPAVRAALPEAIEDVRAGRLAPSVAARRLLDLLDRGPGI"
BASE COUNT          715 a         2156 c         2227 g          712 t
ORIGIN      
        1 cgacgcgcct gccggccagc gtctcgcccc gccgggcggc aagggtcagc cacgcggcgg
       61 ccatgtactc ggcgacggcg gcagcgttgc acccgcgggc ggcgtagaac gcgatgccga
      121 ggcgctcgag atcgggctct gccacgtgat cggtgcccga cgtggcggtg ccgacgaagc
      181 gcacgggcgt cgccgcgaga agcgccgcgt ccacgcgggt gatcgagcgc acgacgagca
      241 gatccacgtc cgcgaggccg gccggcagga tccggcggcc gtgctccagg cgcacgtcgc
      301 cgagagtgcc gaaggcctcc cgggcgaacg ggacgttctc gtctgcgagg atcgtcagac
      361 gcggcatgcg gcgattgtgc cgctcgtggc gggcgccgcg caagcgccgc cggggcggga
      421 cgcgcgccag gccgcgggac gggcggcccg ccgcctcggc cgcccgctca cggctgccgc
      481 gccgcggtga ggcggctgaa gcgggcggtc tgccgctcga tctcggcgag cgccgcctgc
      541 tgcgccgacg acggcgcggc ggctcggatc ggcgggaacg cgcgggtcag gagcgcgccg
      601 gcgtcgcgaa gccgccagcc ttcgtcgggc gaggacggcc gcgcatcgag cacgtcgagc
      661 tggacgcggg ccacggccgt gcgggcgacg gcgagatcga gcgcggcggc ggccggcgct
      721 ccgggagcgt ctgcgaggcg ctcgcggatg cgggcgtact cgcgggcgtg cctcagcgcc
      781 tcgtcgagcc ggcgctggga gcggcacagc tccgccagcg acgcgcgcgc ggccgcagcg
      841 atctccgccg cggccgcgtc gtcggcgccg ccgtcctcgg aagcctgcgc gtcggccagc
      901 gcctggagcc acagcgactc ggccttggca tgatcgcctc ggcgcatggc cacgtccgcc
      961 aggccgccgg tcgaggcagc gaggccgcgc cgggccgact cgtcgccggg ctgtccggcg
     1021 agatcctcgc gctccaggag ctgggccgac gcaaagcgcg cgtcggcgtc gtcgagggcg
     1081 ccgtccgcga gcaggaagcc cccgagcgcg cgcaagacgc cggcgtagtc ccgccgggcg
     1141 gcggccggca gcacgttctc ggcggccagc gtctcgaaac cggccgcggc gtaggcgtac
     1201 atcatccggg cgccggccag gtcgccgcgt gcggcgcgga acgcgcccag ccgcccgtac
     1261 ccggacgacg ccgccgcgcg ggccagtgcg tcccgcggct gctggcgcgc gaggcgctcg
     1321 accagtccca agtggcgagc gtccgccctc ccggcggcct cgtcgtcgcc gagctcctgg
     1381 cgcagcgccg ccgcatcacc gcacgccgcg atgagcgcga tggccgcctc gaccgattcc
     1441 ggctcggccg cgagggcccg ctcgcctgcc gcgacggccg catcgaggct ggcggcggcc
     1501 gaggcgcggc ccgccggcgc gccgccggac gtagccgcct ggagtgcggc gacgagccgg
     1561 taggctcggg ccagatcgag cgccgcggcc ggatcgccgg ccgcatcgcg cgcgaggccg
     1621 tccacctgtt cgacggcgcg cgtcagaacg gccgtccgcg cgggagcaga tgccgaaagc
     1681 gcggcaatgg cctcgtgcag ctcgttcagc agcgaccgcg agaattgctg cgacaccctc
     1741 agccggcccc cggcgagatc ccgctccgcg cgcagtgccc gcgcttgcca gccgaacagg
     1801 gccgtccccg cgccaagggc ggcgatggcg gcccccagga cggccgccag aagccagtgc
     1861 cggcgtagcg ccttcgccgc gcgttgacgc cggctcgccg gaaacaccga tacggtccgg
     1921 ccgtggagcc acgcgcggag gtcgtcggcg aaggccgtga ccgactgata gcgcatgcgc
     1981 gggtccttgc gcagcgcctt catgacgacg gcgtccagat cgttcgccag ggcgcggcgc
     2041 tgcgacggcg gcgcgacccg gctcggcggc tcggggtccg tgtcgcagat cacccgcgtc
     2101 atctcgaccg gcgtcagatc ccggaactcg tagggcgggt ggtccgtcag cagaacgtac
     2161 aggagcacgc cgagcgagta gatgtcgctc gccgcggtga tgagctctcc gcgcgcctgc
     2221 tccgggctga cgtattcggg cgtgaagagc gactgccccg tgcgcgtgag cccctcgccc
     2281 gagtcgggcc ggccgaacag gagccgcgcc acgccgcagt tcatcacctt gagctggccg
     2341 tccgcggtca ccaggatgtt cttcgggccc acgctgcggt ggacaatcag gcgctggtgc
     2401 gcgtactcga gcgcggtgca gacgcggagc acgagcctca cgcggtcggg caggctggtc
     2461 tcgcggctgc ggcagtaggc gcagatgtcc tctccggcga cgtgctccat cgccacaaac
     2521 ggcatgccgc cctcggccac gccgccgtcc aggaggcgcg cgacccacgg atggtcgagg
     2581 ccggccacga ggtggcaggc ggcgcggaac cagtcagtcc acgcagccgt gttccatccg
     2641 ggcggcagga cgcgaagcgt gacctgctgc ggcgaggccc cggactggtc ggccgcttcg
     2701 tacaccgccc ccatcccgcc gcgcccgacc tgccggatga tgcgataggc gccgaagagg
     2761 gcgccgggag ccagttccgg cgccgtctca ggctggccgt ccggcggctc acggagcacg
     2821 ggagtccggc gcagcgcaga ggcggcaggc gggctcgcgg ggggcgtcgg gcgcgggctc
     2881 tggggctgga tcgacggcgg ccgcggcagg gcgtgcgcgc gcggcgcttg cggctgcagc
     2941 gaggcggccg atggcgcggc ggcggacggc gtggcggcgg acggtgccgc ggcggacggt
     3001 gccgcggcgg acggcgcggc ggacggcgcg gcgtgcggct cgccgcgtgc gggaggcaga
     3061 ggccgggcag tcggccacgt cggcgccggg ccgccgggac gagccgccgg gcctccggga
     3121 cgagccgccg atcctggttg cggacgggac gccgcccgct gcacgagcgc cgcaggcggc
     3181 tcgtcggcgg cggccaccac gggcagacgc ggcggcgctg gtgagggcgc cgccgcggct
     3241 cgaggcggcc cgatgggagc ggcgctcggc tcgtccacga accacgggtc cgagtcgtgc
     3301 tcgtgcagca gcgcccgcac ctcggcccgc agccgctcat cgtcggtcgc accgagcagg
     3361 aacgtctcgc gctcgccggc gggctgctgc agcgccgctt cgaacagctc gtcaatgcgt
     3421 tcccagccgg ttcgttccat cggcaggacc cgtgtgccag tcgagccgcg cgtccgtctg
     3481 cgcgggccag ccgcgcggcc cgctgacgcg ccggtcgacg cttcgcaagc cggacagccg
     3541 tctctatact agtccaggtc acatgaggcg gacgggatcg cgggcggacg gcccgccaca
     3601 tggcgcgcag ctgcggctgc ccttcggacg gtccgcgcgg gagacgacgc gcggcgctgt
     3661 cgtgtcggtg cccggcgtcg gagcgggcgt cgtgttcgtg cgccatcgcc gcgcgcgcca
     3721 gtacgtgctg cgcctgcgcg acgacgggag cgtgcgcgtt acgctgccgt ggagaggatc
     3781 gcgagccgag gcgcagcgct tcgtgcgcga gcgccgcgac tggatcctgc gccagcggct
     3841 gcagcatgcg gcgagcgccg cggcgcgaag cgggccgtgg ctgccgggca cctcggtgct
     3901 gctgcggggc gtggaagagc cgatccgggt ccagccggcc gggggcgcgc tggcggtgac
     3961 gctcggcagc gagcgcctgg tgctgccggc ctcagcctgt cacaacctcc gtccgcccct
     4021 cgaagcccat ctccgatcgg tcgcggcccg cgagttgcgc ggccgcctcc acgaacttgc
     4081 gcgcgcgcac ggcttcacgg tggcggccgt cagcatccgc gcccagcgca cccgctgggg
     4141 atcctgctcg cgatcggggc gcatctcgct gaactggcgc ctcatccagg tgcccgcatc
     4201 cgtccgcgac tacgtgctgc tgcacgagct ggcgcacctg aagcacgccg atcactcggc
     4261 gcggttccac cgcgaggtcg agcgtctctg cccgcactgc gccgaggcgc gtcgctggct
     4321 gcgcgagacg acgcttctcg ggcgctgagc ccgccgggcg gaggtctcta ggcaccggga
     4381 gccggctcag cgcttctccg gccgtctgcc cccgaggcag gtacaatgga cagggatgcg
     4441 ggggtgacag ggatggacag cctcttcagc gaaggaacgc ggcgcggcgc tcgcggggcg
     4501 ctcgtcgcgg cggcgctcgt cgttgcggcg ctcgtcgttg cggcgtcggc cgttcccgcc
     4561 ctggcgcagg acggaccggg actgcgcgcc gggctcagcg ccagcccgga tcagatcttc
     4621 ttcggcgggc actacgtgac gcagccgctc accggcatgc tgcgcttcca gccgaacctg
     4681 gaggtgggcc tcggcggcgg ccagacgacg atcgccttca acggcgagtt cgcgctgtgg
     4741 cggaagatct cgccggactg gcacgtctac ctcggcggcg ggccgtcgat cctcgcggtc
     4801 acgcaccagc accgcgacga caccgacatc gacccggggt tcaacctggt ggcgggcctg
     4861 cgccggcgcg gcggggcctt cttcgagctg aagatcggca ggatcgacag cccctcgttc
     4921 aaggcgctgg tgggcttcac catccgctag gcgccggccg ggtccggcgg cgcggcctcc
     4981 ggctggccga tgagaccccc gggggcgtca gatcccgggt ccccggtcca gcaggtcgag
     5041 cagcctcctt gcggcaaccg acggggcgag gcggcccgcg cgcacgtcct cgatcgcctc
     5101 cggcagcgcg gcgcgcaccg cggggtgcgc gcggaaccgc tcgcgcagcc cggcctcgac
     5161 gcgctccttc atccacgcct cggcctgctg ccgccgccgc agcgcgaacc ggccggaggc
     5221 ctgctgcatc tcccggaacc gcgagatcgc gtcttcgaaa gcgtccaggc cctgcccggt
     5281 gagggcgctc accgtcagca cctccgggtg ccaggcggcc gcatcgaggg gttcctcctc
     5341 gccgcgcccg tgcaggccga gtattcgcag cgacgaggtg atctgggccc gcgcgttcgt
     5401 ggcggccgcc tcgtcgaggt cggccttgtt gatcgcgatg agatcggcca gctcggtgat
     5461 ccccttcttg atcgcctgca gatcgtctcc cgcgttgggc agctgcagca ggacgaacat
     5521 gtcggtcatg ccggcgactg ccgtctcgct ctgtccgacg ccgacggtct cgatgatgac
     5581 gacgtcgtgg ccggcagcct cgaccgccag catcgcctcg cgcgtcttgt cggcgacgcc
     5641 gccgagcgtc cccgacgagg ggctcggccg aatgaacgcg cgctcgtcgg cggagagccg
     5701 ctccatgcgc gtcttgtcgc cgaggatcga acctccggac acgctgctcg acgggtccac
     5761 cgccagcacg gccacccgcc ggccctgcgc gaggaggcgc aggccgagcg
//
LOCUS       DAOSIF010000151         5758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12590_length_5758_cov_4.647554, whole genome shotgun sequence.
ACCESSION   DAOSIF010000151 DAOSIF010000000
VERSION     DAOSIF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12590_length_5758_cov_4.647554"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /gene="lpxD"
                     /locus_tag="PLT35_06480"
     CDS             <1..841
                     /gene="lpxD"
                     /locus_tag="PLT35_06480"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOG28913.1"
                     /translation="GAVIAGAFAAQYAGPKPVLVAKQPKLAFARAVKLLAGAPPATGG
                     VHASAMVAPTAALGQSVSIGPHAVVGEGVAIGDRTQVGAGVAIGDGVRIGPDCRIHPN
                     VVIYAGTEVGARVVLHAGVVLGSDGFGYVMDEETGAYEAFPQIGTLRLEDDVEIGANT
                     TIDRGALGATVIGAGTKIDNLVQVAHNVRIGRHVAISAQTGIAGSSSIADRVIVGGQA
                     GIADHVRIEEGVILGAQAGVPTSKILRGKGVVFWGSPARPLPGVLREQATLARLARKR
                     GRE"
     gene            complement(815..1837)
                     /locus_tag="PLT35_06485"
     CDS             complement(815..1837)
                     /locus_tag="PLT35_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOG28914.1"
                     /translation="MARHVRRLVGPLAPLAGAGLLLAALAAAPAARAGAAPRAAPAGG
                     PWLFSYFVDDGQDGLHLAYSRDGLAWTPLREGRPFLAPTVGGKLMRDPSVVLGPDGVF
                     HMVWTTGLWEQGIGVGHSRDLLAWSGVEFLPVMVHERQAVNAWAPEIIWDEETRQYVI
                     FWATSIPGRFPDTDESADSRSSDGARLNHRIYRTTTRDFREYTRAELFFDPGFAVIDA
                     AIVRDGAQYVMVFKNATRFPEATEDIRIAFADHALGPYRVEPGPISKANRVEGPTAFR
                     AGGELMVLYDAYTRRRFEGVRSRDLKAWAALGADLTMPAGARHGTVFAVPEKVLAGLL
                     AAPLAR"
     gene            2268..4112
                     /locus_tag="PLT35_06490"
     CDS             2268..4112
                     /locus_tag="PLT35_06490"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28915.1"
                     /translation="MITSRTSRYSASPVLTLLAGLALALLMASTAGAQVVSNPRIAEF
                     DPSADHWTDLGGGGPAVLRYELGVYMVGASAPFTTVDMGKPSPEADGKIRYDFSSSVS
                     GWPLPGGSYEARVAAVGPEGSALSEPSNPFTFGVAASPCTFALGATSASIPVTGGRYA
                     VDVQTGDGCSWSVSTTSSWIALTTLNGSGSSTVPFDVLANSSTASRAGSIRIGGQTLV
                     LQQAGLATPTLSWAKPAAITQGTPLGAAQLNATASVPGTFAYNPAAGTVLAAGTRTLN
                     VTFTPSDPTRYTTATASTTITVNAVSYTLTIARPANGTVMGSGINCGSGGSACSVTMP
                     GPMTIGLQAVADTGYTFTSWSGSCSGASPTYSLSLNGPVSCGAAFAAVPAAPPAAPPA
                     APPAEPPAEPPPASPPPASPAPPSGGLPIGAPYTLTIQRQSGGVVRGAGINCGTTARA
                     CTTTMPGPMTLGVQATPDPGYRFVEWTGHCSGSNASYAIALEGARTCAASFVPAGSAV
                     IAPPPATEPVPSPPPAPSPSAGPPYRLNITRPSGGTIRAAGINCGTGGSACSVTMPAP
                     LWLGILATPDRGYRFVGWTGDCSGAHAAYALSLAGTRTCGATFVASGN"
     gene            4548..5606
                     /locus_tag="PLT35_06495"
     CDS             4548..5606
                     /locus_tag="PLT35_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG28916.1"
                     /translation="MYEAFFGLNERPFQLTPNPRFLFLNPRQREALATLRYGFTSSLG
                     ITLLLGEAGTGKTTLLRAALDAESRPEYRHVVLNNPTLSPGEFYEILADRFALPAASG
                     SKAKFLLAFERDLAARHAAGGLTAIVIDEAQSLTHELFEEVRLLANLETATAKLVNVV
                     LVGQPELADRLNDPSLRQLKQRVVLRCSLEPLDRHWTACYIASRLRVAGGEPTEVFAK
                     DAVLAIYEASRGIPRTIGVVCENALLAGYAAQKKPVDGPLVAEVCGDLDLASSNGRPR
                     RAGEPLIEPPADENGGADERAARPRAALRAAAQARTRRREPCAWLPPKDAAAPPDGPG
                     RAQDAPANPDPNRIFRFF"
BASE COUNT          806 a         2221 c         1986 g          745 t
ORIGIN      
        1 cggcgccgtc atcgccggcg cgttcgccgc gcagtacgcc ggcccgaagc ccgtgctggt
       61 ggcgaagcag cccaagctgg cgttcgcccg cgcggtgaag ctgctcgccg gcgccccgcc
      121 ggctacaggc ggcgttcacg caagtgccat ggtcgcgccc acggcggccc tcggccagtc
      181 ggtgtcgatc ggcccccacg ccgtcgtcgg cgagggcgtc gccatcggcg accggacgca
      241 ggtcggggcc ggcgtcgcca tcggcgacgg cgtccgcatc ggcccggact gccgcatcca
      301 tccgaacgtc gtcatctacg ccggaaccga agtcggcgcg cgggtcgtgc tgcacgccgg
      361 cgtcgtcctc gggagcgacg ggttcggcta cgtgatggac gaggaaaccg gcgcctacga
      421 ggcgttcccg cagatcggga cgctgcgcct cgaagacgac gtcgagatcg gcgcgaacac
      481 gaccatcgac cgcggcgccc tcggcgccac cgtcatcggc gccggcacga agatcgacaa
      541 cctcgtgcag gtggcgcaca acgtgcggat cgggcgccac gtggccatct cggcccagac
      601 cgggattgcc ggcagctcca gcatcgccga ccgcgtcatc gtcggcgggc aggccggcat
      661 cgccgaccac gtccgcatcg aggagggcgt gatcctcggc gcgcaggcgg gggtgccgac
      721 cagcaagatc ctgcgcggca agggcgtagt cttctggggc agccccgccc gtccgctgcc
      781 gggcgtcctg cgggagcagg cgacgctggc gcgcctagcg cgcaagcggg gccgcgagta
      841 gccccgccag caccttctcc ggcaccgcga agaccgtgcc gtgccgcgcc ccggccggca
      901 tcgtcaggtc cgcgccgagc gccgcccacg ctttcaggtc gcgcgaccgc acgccctcga
      961 agcggcggcg cgtgtacgcg tcgtagagaa ccatcagctc gccgccggcg cgaaacgccg
     1021 tcggcccctc cacccggttc gccttcgaga tcggcccggg ctcgacgcgg tacggcccaa
     1081 gcgcgtggtc cgcgaacgcg atgcggatgt cctccgtcgc ttcggggaac ctcgttgcgt
     1141 tcttgaagac catcacgtac tgggcgccgt cgcggacgat cgcggcatcg atgacggcga
     1201 agccgggatc gaagaacagc tccgcccggg tgtactcccg gaagtccctc gtcgtcgtcc
     1261 ggtagatgcg gtggttcagc cgcgcgccgt ccgacgagcg gctgtcggcg ctctcgtccg
     1321 tgtccgggaa gcggcccgga atcgacgtgg cccagaagat gacgtactgc cgcgtctcct
     1381 cgtcccagat gatctcgggc gcccacgcgt tgaccgcctg acgctcgtgc accatgaccg
     1441 gcaggaactc gacgccgctc caggcgagca ggtccctcga gtggcccacc ccgatgccct
     1501 gctcccacaa gcccgtcgtc cagaccatgt ggaagacgcc gtcggggccc agcactacgc
     1561 tcgggtcgcg catgagtttt ccgccgaccg tgggcgcgag gaacgggcgg ccctcgcgaa
     1621 ggggcgtcca ggcgaggccg tccctgctgt aggccaggtg caggccgtcc tgcccgtcat
     1681 cgacgaagta gctgaagagc catggcccgc cggcgggcgc cgcacgaggc gcggctcctg
     1741 cccgggccgc aggcgcggcg gcgagcgccg cgagaagcag cccggcgccg gcgagcgggg
     1801 cgagcgggcc gacgaggcgt cgaacgtggc gtgccatgcg cccatgctac ccgagcgcgc
     1861 gccgcgcgca gaagggcccc ggcccgcagc ggtctccccc tcgtcctgag aacgaatagg
     1921 ggtctggctc cggaacagga tcagatcccg gtgcctgacc ccttttcgcg accgcattct
     1981 cgtcaaggga atgacgcgac gacgagaagc gtcaacggcg ccgcggcgca cccggcgcgc
     2041 caggcccgcc agacgccgcg cgccgggccg ggacagaact gtaatccgtc cgcagaaacc
     2101 ggaaccagcg tgcagtcctg cgcgttggat tcgcgccgaa taccggccaa ttccagcggt
     2161 tcccgcacgg tctgcggtcc aggcgccggg gcatgtttct tgcgccctcc gcgccgaagc
     2221 cgaaggggac gcccgtctcc gggcacgcca acgcgcggga ccgcaccatg attacttctc
     2281 gaaccagccg atacagcgca tcgccggttc tcacgctcct cgccggcctg gcgctcgcgc
     2341 tattgatggc ctcgaccgcc ggcgcgcagg tcgtctcgaa cccgcgcatc gcagagttcg
     2401 atccgtccgc ggaccactgg accgacctcg gcggcggcgg gccggccgtg ctgcgctacg
     2461 agctcggcgt ctacatggtc ggggcgtccg cgccgttcac caccgtggac atgggcaagc
     2521 cgtcgcccga ggccgacggg aagatccgct acgacttctc gtcgagcgtg agcggctggc
     2581 cgctgccggg gggcagctac gaggcccgcg tggccgcggt cggccccgag ggatcagcgc
     2641 tcagcgaacc gtcgaacccc ttcacgttcg gcgtcgccgc cagcccctgc acctttgccc
     2701 tcggcgcgac gagcgcgtcc atccccgtca ccggcggccg ctacgcggtg gacgtccaga
     2761 ccggcgacgg ctgctcgtgg agcgtctcga cgacctcgtc ctggatcgcg ctcacgacgc
     2821 tgaacggctc gggcagctcg acggtcccgt tcgacgtcct tgccaactcg tccaccgcca
     2881 gccgcgccgg ctcgatcagg atcggcggac agacgctcgt gctccagcag gccggcctgg
     2941 cgacgccgac gctgtcctgg gcgaagcccg cggccatcac gcagggcacg ccgctcggcg
     3001 cggcgcagct caacgccacc gccagcgtgc ccggcacctt cgcctacaac ccggccgccg
     3061 gcacggtgct cgccgccggc acgcggacgc tgaacgtcac cttcacgccg tccgacccga
     3121 cgcgctacac gacggccacg gcctcgacca caatcaccgt caacgcggtc agctacacgc
     3181 tcaccatcgc gcgccccgcg aacggcacgg tcatgggctc cggcatcaac tgcggcagcg
     3241 gcggcagcgc ctgctcggtg accatgcccg ggccgatgac catcgggctg caggccgtcg
     3301 ccgacacggg ctacacgttc acctcctggt cgggcagctg ctccggcgcg tcgccgacct
     3361 actcgctgtc gctcaacggc ccggtctcct gcggcgcggc gttcgccgcg gtcccggccg
     3421 ccccgccggc cgccccgcca gccgcaccgc cagccgaacc gccagccgaa ccgccgccgg
     3481 cctccccgcc gccggcctcg cctgcgccgc ccagcggcgg gctgccgatc ggcgccccgt
     3541 acaccctcac gatccagcgc cagtctggcg gcgtcgtccg gggcgcgggc atcaactgcg
     3601 gcaccaccgc cagggcctgc acgacgacga tgcccggccc gatgacgctg ggtgttcagg
     3661 ccacgcctga tccaggctac cgcttcgtcg aatggaccgg gcactgttcg gggagcaacg
     3721 cgagctacgc gattgcgctc gagggcgccc gcacctgcgc cgcgagcttc gtcccggccg
     3781 gcagcgctgt catcgcaccg ccgccggcaa ccgagccggt cccgagcccg ccgccggctc
     3841 cgtcgccgtc cgccggcccg ccgtacaggc tgaacatcac gcggccctcg ggcggtacca
     3901 tcagggccgc cggcatcaac tgcggcaccg gcggcagcgc ctgctcggtc accatgcccg
     3961 cgccgctctg gctggggata ctggcaacgc cggacagagg gtacaggttc gtcggctgga
     4021 cgggcgactg cagcggcgcg cacgctgcct acgccctgtc gctcgcgggc acgcgcacct
     4081 gcggcgccac cttcgtcgcg tcgggcaact agccgcaccg accggctcgt cgcaccgaag
     4141 cggctcgccg cgccgggcgg gccgcctgtc gctgctgcgc cgaaggcggc atcccacagc
     4201 cggacatggc acgccgcagg gccccggacg gggccctgcg cttgacaaag ccggggctcg
     4261 ggcctaggct cgcatgaggg gtgtgaccga tgacaatgcc gccggcgtga gcccgcctgg
     4321 ccttggccgc gcgggctctg ggaagttgcg tgcagtcatg gtcttgcgac gacgggcagc
     4381 cggcgattca ggagcaggcg gggtgttgaa cgcgcgcgcc tcggccgata tctcacccga
     4441 gtcagtcgag ggggcgcgat cgcaccccct ggagcggttc gcgcgcgcgt ccattcaggc
     4501 cctgacatgg gactgacggg gcgaagccgt ctcccggccg gttgcagatg tacgaagctt
     4561 tcttcggatt gaacgagcgg cccttccagc tcacgccgaa cccgcggttt ctcttcctga
     4621 acccgcggca gcgcgaggcc ctcgccaccc tccgctacgg gttcacctcg tcgctgggca
     4681 tcacgctgct cctcggcgag gccggcacgg gcaagaccac gctgctccgg gctgccctcg
     4741 atgccgagag ccggcccgag taccggcacg tcgtcctgaa caacccgacg ctgagcccag
     4801 gggagttcta cgagatcctc gccgaccggt tcgcgctgcc cgccgcctcc ggatcgaagg
     4861 cgaagttcct cctcgccttc gagcgcgacc tcgccgcgcg ccacgcggcc ggcggcctca
     4921 cggccatcgt gatcgacgag gcgcagagcc tgacgcacga gctgttcgag gaggtccggc
     4981 tgctcgccaa cctggagacc gccacggcga agctcgtcaa cgtggtgctc gtcggccagc
     5041 ccgagctggc cgatcggctg aacgacccgt cgctgcgcca gctcaagcag cgggtcgtgc
     5101 tccgctgctc gctcgagccc ctcgatcgcc actggaccgc gtgctacatc gcctcgcgcc
     5161 tgcgcgtggc ggggggcgag cccacggagg tgttcgccaa ggacgccgtc ctggccatct
     5221 acgaggcctc gcgcggcatc ccgcgcacga tcggcgtcgt gtgcgagaac gcgctgctgg
     5281 cgggatacgc ggcgcagaag aagcccgtcg acgggccgct cgtcgccgag gtctgcggcg
     5341 acctcgatct cgcctcgtcg aacggccggc cccgccgcgc cggcgagccg ctgatcgaac
     5401 cgccggccga cgagaacggc ggcgccgacg agcgcgccgc gcggccccgc gcggcgttgc
     5461 gcgccgccgc gcaggccagg acgcgccgcc gcgagccatg cgcctggctg ccgcccaaag
     5521 acgccgcggc gccgccggat gggccgggcc gggcccagga cgcgccggcc aatccagacc
     5581 cgaaccggat cttcaggttc ttctgacagg actgacgctc atgcgcacga taccggccgc
     5641 cctcgtcgtg ttggcacttc tcgcgcccgc cggccttggg gcccggggtg gcgcccaggc
     5701 tggcgcccag gcgcaacccg ccgcgaagcc ggccgcaggc caggccgcag ggcaggcc
//
LOCUS       DAOSIF010000152         5754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12608_length_5754_cov_3.301807, whole genome shotgun sequence.
ACCESSION   DAOSIF010000152 DAOSIF010000000
VERSION     DAOSIF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12608_length_5754_cov_3.301807"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="PLT35_06500"
     CDS             <1..1529
                     /locus_tag="PLT35_06500"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOG28917.1"
                     /translation="GLQCPAMVRTGFDTDRYLAEQTAAIRERVRDCGDRLYLEFGGKL
                     LFDYHAARVLPGFDPNVKMRLLQELGDDVEVLLCIFAGDIERRKVRADFGITYDSDAL
                     KLIDDLRRWGIEVSAVVITRYDEQPAARVFKNTLERQGLRVFTHGVTKGYPTDVDTIV
                     SERGYGANAYVPTSKRLVVVTAPGPGSGKLATCLSQLYHEHQAGRTAGYAKFETFPIW
                     NLPLKHPVNIAYESATADLRDFNLVDPFHLEAYGRTAVNYNRDVEIFPVLRRILERIL
                     GRAPYRSPTDMGVNRAGFGIVDDEAVREAARQEAIRRYFRASCEYLMGFVNADTVQRT
                     ELLMEELGVAPRDRAVVQPAEDAAKRARETGKGNNGIYCGAAIELPGGAVVTGTNSPL
                     MHAASSLVLNAVKHLAGIPDPIHLLSPSVIGAVAHLKQNVLASSSISLDLEETLIALT
                     VGAATNPTAQLAMEKLKALAGCDAHMTLMPSRGDEAGLRKLGVNLTTDPVLPRRSLLH
                     "
     gene            complement(1480..2937)
                     /locus_tag="PLT35_06505"
     CDS             complement(1480..2937)
                     /locus_tag="PLT35_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG28918.1"
                     /translation="MGGFFGVVSRHDCVADLFYGTDYHSHLGTSRGGMVVRNGNGFKR
                     NIHDISGTPFRSKFSEDLPKYDGGSGIGVISDNEDQPLLIASHHGSYAVVTVGRIENV
                     DELARQTLQSRAVHYSELKGNAFNPTEVVATIINGQGSLVEGLREVQARVLGSCSVLL
                     LTDHGLYAARDAFGRTSVTLGRKDDAWAVTMETCALPNLGYEVERHLGPGEIVRVTAE
                     GHETLAAPGDRMQVCAFLWIYYGYPASNYEGINTEVARYRCGEALARADATRPDLVAG
                     IPDSGIGAAIGYAAEAGVPYRRPFVKYTPTWPRSFMPQEQRVRDLVARMKLIPIREII
                     EGRRLLFCEDSIVRGTQLRDLIRRVFDAGAREVHMRPACPPLVYGCRFLNFSQSRSEL
                     DLAARRAVTALEGRADARLEEYADPATDRHCAMVEWIRRSLGLTTLQYQRLGDMVAAI
                     GLPRERLCTYCWTGDAPFGVNAGGFSAAAPDRSSG"
     gene            complement(2954..4471)
                     /locus_tag="PLT35_06510"
     CDS             complement(2954..4471)
                     /locus_tag="PLT35_06510"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOG28919.1"
                     /translation="MLPEFFSSMLELIVRTSTELPPDVRAAMKAATDLEPPDTRSGQA
                     LSIIGVNIDQAAGVEGPICQDTGMPFFEIHLPRGSDQLWMADEIRRAVAEATRLGKLR
                     PNSVDSITGKNSGDNLGPGTPIFHFHQWAREEVGVKLLLKGGGCENCNAQYSLPMDLP
                     HLGRADRNLEGVRKCILHAVWAAQGQGCSPGAIGACVGSDRAQGYLYAKGQLFRTLDD
                     VNPEPALAELEADIMGAANRLGIGTMGFRGRTTLIGCKITAMNRLPASFFVSVAYECW
                     AFRRLGVILDAATGKIVEWQYRDPSSPLIPMADQAGYRRTGREVSLTTPLTEDAVRAL
                     KVGDIVLLSGRMFTGRDAVHAYLMKHDSPVDLNGSALYHCGPVAVKDGDRWVMKAAGP
                     TTSIREEPYEADVIRNFGVRAVIGKGGMGARTLEALKERGAVYLNAIGGAAQFYARCI
                     ERVDDVSLFHFGVPEAMWHLQVRDFPAIVTMDAKGNSLHKDVEAESARELAALRR"
     gene            4517..5215
                     /locus_tag="PLT35_06515"
     CDS             4517..5215
                     /locus_tag="PLT35_06515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG28920.1"
                     /translation="MPRRISSRVSARESRLGAAATAAALFVLACAASSGAQERPRHGK
                     LFAPVDLGLLEGPDRDLWQMPDRIMDALAIAEGSVVADIGAGGGWFTIRLGRRVGSNG
                     TVYAEDIQRQMLEATSRRAAREGLTNVRTVLGTSEDPRLPKGALDAVLIVDTYHELEN
                     PVAMLRNIAASLKAQGRVGVVDFKRDGLGPGPALEERVDPELVIRDAAEAGLALLRRE
                     TFLTYQFMLVFGRR"
     gene            5284..>5754
                     /locus_tag="PLT35_06520"
     CDS             5284..>5754
                     /locus_tag="PLT35_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOG28921.1"
                     /translation="MPDALLSLTPEPLDATALVRLLDDASAPGEHGAVTAFVGMVRAE
                     NLGRRVIALDYEAYEPLALEAFRLIAEEAAAAWPGVRLAIRQRVGRVGVGDASIVIAA
                     ASAHRAEAFAACRYAIERVKQVAPIWKRELFAGGACWLEGRTADPFDAAARDAAM"
BASE COUNT          847 a         2044 c         1997 g          866 t
ORIGIN      
        1 tggggctaca atgcccagcc atggtccgaa ccgggttcga caccgatcgc tacctcgccg
       61 agcagaccgc cgccatccgg gagcgcgtcc gcgactgcgg cgacaggctg tacctcgagt
      121 tcggcgggaa gctgctgttc gattaccacg cggcgcgcgt gctccccggc ttcgacccga
      181 acgtcaagat gcgcctgctc caggagctgg gcgacgacgt ggaggtgctg ctctgcatct
      241 tcgccggcga catcgagcgc cggaaggtcc gggcggactt cggcatcacg tacgactccg
      301 acgccctcaa gctcatcgac gacctgcggc gctggggcat cgaggtctcg gccgtggtca
      361 tcacccggta cgacgagcag ccggccgcgc gcgtgttcaa gaacacgctg gagcgccagg
      421 ggctgcgggt gttcacgcac ggggtcacga aggggtatcc gaccgacgtc gacacgatcg
      481 tgagcgagcg cgggtacggc gccaacgcgt acgtcccgac gtcgaaacgg ctggtggtcg
      541 tgacggcgcc gggccccggc agcggcaagc tcgccacgtg cctctcgcag ctgtaccacg
      601 agcaccaggc cggccgcacg gcgggctacg ccaagttcga gacgttcccc atctggaacc
      661 tgccgctgaa gcacccggtg aacatcgcct acgagtcggc gaccgccgac ctccgggact
      721 tcaacctcgt ggatccgttc cacctggagg cgtacggccg gacggcggtg aactacaacc
      781 gggacgtgga gatcttcccc gtgctgcggc ggatcctcga acggatcctc gggcgggcgc
      841 cctatcggtc gcccaccgat atgggcgtca accgggccgg gttcggcatc gtggacgacg
      901 aggcggtgcg cgaggcggcc aggcaggagg cgatccgccg ctactttcgc gcgtcgtgcg
      961 agtacctcat gggcttcgtg aacgcggaca cggtgcagcg gaccgagctg ctgatggagg
     1021 agctcggcgt ggcgccccgc gaccgcgcgg tcgtccagcc ggccgaggat gcggcgaagc
     1081 gggcgcggga gacgggcaag ggaaacaacg ggatctactg cggggccgcc atcgagctgc
     1141 ccggcggcgc cgtggtcacg ggcacgaact cgccgctcat gcacgcggcg tcgagcctcg
     1201 tgctgaacgc cgtcaagcac cttgcgggca tccccgatcc catccacctg ctgtcgccgt
     1261 cggtcatcgg cgccgtcgcg cacctgaagc agaacgtgct ggcgtcgagc tcgatcagcc
     1321 tggatctcga ggagacgctc atcgcgctga ccgtcggcgc cgcgacgaac ccgacggccc
     1381 agctcgccat ggagaagctc aaggcgctgg cggggtgcga cgcccacatg acgctcatgc
     1441 cctcgcgcgg cgacgaggcc gggctgcgca agctgggcgt caacctgacg accgatccgg
     1501 tgctgccgcg gagaagcctc ctgcattgac gccgaagggc gcgtcgccgg tccagcagta
     1561 ggtgcacagc cgctcccgcg gcagcccgat cgccgccacc atgtcgccga ggcgctggta
     1621 ctgcagcgtc gtgagcccta ggctgcggcg gatccactcc accatcgcgc agtggcggtc
     1681 ggtcgcgggg tccgcgtact cttcgaggcg ggcgtcggca cgcccttcga gcgccgtcac
     1741 cgcccgccgg gccgccaggt ccagctccga cctcgactgc gagaagttga ggaagcggca
     1801 gccgtacacg agcggagggc aggccggccg catgtgcacc tcccgcgcgc ccgcgtcgaa
     1861 gacgcgccgg atgaggtccc gcagctgcgt gccgcgcacg atcgagtcct cgcagaacag
     1921 gaggcggcgc ccctcgatga tctctcggat ggggatcagc ttcatgcgcg cgacgaggtc
     1981 gcgcacccgc tgctcctgcg gcatgaagga ccggggccac gtgggcgtgt acttcacgaa
     2041 cggccggcgg tacggcaccc cggcctcggc cgcgtagccg atggccgcgc cgatgccgga
     2101 gtctggaatg ccggccacga ggtccggccg cgtggcatcg gcgcgcgcga gggcctcgcc
     2161 gcagcggtag cgcgccacct ccgtgttgat cccctcgtag ttcgacgccg gatagccgta
     2221 gtagatccac aggaacgcgc agacctgcat gcggtcgcca ggcgccgcca gcgtctcgtg
     2281 gccctcggcc gtgacccgca cgatctcgcc ggggcccagg tggcgctcca cctcgtagcc
     2341 gaggttcggc agcgcgcacg tctccatcgt caccgcccag gcgtcgtcct tgcggccgag
     2401 cgtgacggag gtgcggccga aggcgtcccg ggccgcgtag aggccgtggt cggtgaggag
     2461 caggaccgag cacgagccga ggacgcgcgc ctgcacctcc cggaggccct ccaccaggct
     2521 gccctggccg ttgatgatgg tggcgacgac ctccgtggga ttgaaggcgt tgcccttcag
     2581 ctccgagtag tgcacggctc tggactgcag cgtctgccgc gccagttcgt ccacgttctc
     2641 gatgcggccg acggtgacga cggcgtacga gccgtggtgc gaggcgatga gcagcggctg
     2701 gtcctcgttg tcgctgatga cgccgatgcc agacccgccg tcgtacttcg gaaggtcctc
     2761 gctgaacttc gaccggaacg gcgtgcccga gatgtcgtgg atgttccgct tgaacccgtt
     2821 cccgttgcgc acgaccatgc cgccacggga cgtgccgaga tgcgagtggt agtcggtgcc
     2881 gtagaacaag tccgccacgc agtcgtggcg gctcaccacc ccgaagaacc cgcccatcga
     2941 tgcccccggg cgctcagcgc cgcagcgcgg ccagctccct cgcgctctcg gcctccacgt
     3001 ccttgtgcag gctgttccct ttcgcgtcca tcgtgacgat ggccgggaag tccctcacct
     3061 ggaggtgcca catcgcttcc ggcacgccga agtggaacag cgagacgtcg tcgacccgct
     3121 cgatgcaccg cgcgtagaac tgcgccgcgc ccccaatcgc gttcaggtag actgcgccgc
     3181 gctccttcag cgcctcgagg gtcctcgcgc ccatgccgcc tttgccaatc accgcccgca
     3241 ccccgaaatt ccggatgaca tcggcctcgt acggttcctc ccggatgctc gtcgtcgggc
     3301 ccgctgcctt catgacccag cggtcgccgt ccttcaccgc caccggcccg cagtgataca
     3361 gcgccgaccc gttcagatcc accggcgagt cgtgcttcat caagtacgcg tgcaccgcgt
     3421 cccggcccgt gaacatccgg ccgctcagga gcacgatgtc gccgaccttc agcgcgcgga
     3481 ccgcgtcctc ggtgagcggc gtcgtcaagg acacctcgcg gcccgtccgc cggtaccccg
     3541 cctggtcggc catcgggatg agcgggctgg agggatcccg gtactgccat tcgacgatct
     3601 tgccggtggc ggcgtcgaga atcacgccca gccggcggaa cgcccagcac tcgtacgcca
     3661 ccgacacgaa gaagctcgcc ggcagccggt tcatcgccgt gatcttgcag ccgatgagcg
     3721 tcgtgcgccc gcggaacccc atggtcccga tgccgaggcg gttcgcggcg cccatgatgt
     3781 ccgcctcgag ttccgcgagc gcgggctccg ggttcacgtc gtcgagcgtg cggaagagct
     3841 ggcccttggc gtagaggtac ccctgcgccc ggtcgctgcc gacgcacgcg ccgatcgccc
     3901 cggggctgca gccctggccc tgcgccgccc acacggcgtg caggatgcac ttgcgcacgc
     3961 cttccaggtt gcgatcggcg cggccgaggt gcgggagatc catcgggagc gagtactgcg
     4021 cgttgcagtt ctcgcagccg ccgcccttga gcagcagctt caccccgacc tcctccctcg
     4081 cccactggtg aaagtggaag atcggggtgc cggggccgag gttgtcgccg gagttctttc
     4141 ccgtgatcga gtcaaccgag ttcggccgga gcttgcccag cctggttgcc tcggccacgg
     4201 ccctgcggat ctcatccgcc atccacagct ggtcggagcc acggggcaga tggatctcga
     4261 aaaacggcat ccccgtgtcc tggcagatgg gcccctcgac gcccgcggct tggtcgatgt
     4321 tgacgccgat aatcgaaagc gcctgtcccg accgcgtgtc gggcggttcg agatccgtgg
     4381 ccgccttcat cgcggcgcgg acgtcgggag gaagctccgt cgacgtcctg acgatcagct
     4441 ccagcatgct cgagaagaac tctggtaaca tgggtcccga gtataccgtg tttcaccggc
     4501 tcaggctaaa ccactgatgc cacgcaggat aagctcgcgg gtttcggcgc gggagagtcg
     4561 tcttggcgcg gcggcgaccg cggcggcgct cttcgtgctg gcctgcgccg cttcttccgg
     4621 cgcgcaggag cggcccaggc acggcaagct gttcgcgccg gtggatctcg gcctgctcga
     4681 gggcccggac cgcgacctct ggcagatgcc ggaccgcatc atggatgccc tcgccatcgc
     4741 cgagggatcc gtggtggcgg atatcggcgc gggcggcggg tggttcacga tccgcctggg
     4801 ccggcgggtc ggctcgaacg gcacggtcta cgccgaggac atccagcgcc agatgctgga
     4861 ggcgacgagc cggcgcgccg cgcgggaggg cctgacgaac gtccggaccg tgctcgggac
     4921 ctcggaggat ccccgcctgc cgaaaggcgc cctggacgcc gtcctgatcg tcgacacgta
     4981 ccacgaactc gagaaccccg tcgcaatgct ccgcaacatc gcggcctccc tgaaggcgca
     5041 gggacgcgtc ggcgtcgtgg acttcaagag ggacggcctc gggccaggcc ctgccctgga
     5101 ggagcgcgtc gatcccgagc tcgtgatccg cgacgccgca gaggcgggcc tcgcgcttct
     5161 ccgccgggag acgtttctca cgtatcagtt catgctggtc ttcgggcggc gctgagcctg
     5221 gcctgcggtg cggcccgcgc gccgcggccc gaagccggct gctatactga ggcgttggct
     5281 gccatgccgg acgccctgct gagcctgacg ccggagccgc tggacgcaac cgcgctcgtg
     5341 cgcctcctgg acgacgcgtc tgcgcccggg gagcacggcg ccgtcaccgc gttcgtgggg
     5401 atggtgcgcg ccgagaacct cggccggcgc gtcatcgcgc tcgactacga ggcgtacgag
     5461 ccgctggcct tggaggcctt ccgcctcatc gcggaagagg ccgcggcggc gtggcccggc
     5521 gtccgcctgg ccatccggca gcgggtcggg cgcgtcggcg tcggtgacgc gagcatcgtc
     5581 atcgcggccg cctcggcgca tcgggccgaa gccttcgccg cctgccgcta cgccatcgag
     5641 cgcgtgaagc aggtcgcgcc gatctggaag cgcgagctgt tcgcaggcgg cgcctgttgg
     5701 ctggaaggcc ggacggcgga cccgttcgat gccgcggccc gcgacgccgc gatg
//
LOCUS       DAOSIF010000153         5752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12615_length_5752_cov_3.981745, whole genome shotgun sequence.
ACCESSION   DAOSIF010000153 DAOSIF010000000
VERSION     DAOSIF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12615_length_5752_cov_3.981745"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..683)
                     /locus_tag="PLT35_06525"
     CDS             complement(150..683)
                     /locus_tag="PLT35_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOG28922.1"
                     /translation="MEQTLLLNASFEPLKVVHWKKAVTLLCQGKVEVISVYDRDVRSV
                     SFTIKLPSVIRLLRYVRIKHRFDYVPFSRANIYARDEHTCQYCGRRLPTSDLTFDHVV
                     PVAQGGTKDWENIVTCCVACNRKKGGRTPAGAGMRLIRLPKRPESAPALRITIGFRYA
                     PESWRDYLYWNVELDDE"
     gene            758..1348
                     /locus_tag="PLT35_06530"
     CDS             758..1348
                     /locus_tag="PLT35_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOG28923.1"
                     /translation="MAYCRTRGAACAAIAGAALAAACAGQQPRVRVLPPRAEPAAEAP
                     APGRPSPALPPADGGGAAFAGEAIARTAERLLGAPYRDGGAQPDGFDCSGLVSYVFAR
                     HGIPVPRDVRRQAVAGIEVEPAEVLPGDLLFFATTGSGPTHVGIAMGGGRFIHAPKNG
                     AVVRMESLSSTYWSSRFVSARRLLGSGPVKRGQVST"
     gene            complement(1356..2165)
                     /locus_tag="PLT35_06535"
     CDS             complement(1356..2165)
                     /locus_tag="PLT35_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28924.1"
                     /translation="MIDYTDHVHRLMADIVSRVPALSHVDVNRVLVFARPGRTGAHGP
                     YSTCHSLLVPDSEPAYYYWRDPRTGAMAKRSEWFVVRSPEVRIGPRRIWHLVSVALPR
                     FCDQAAADTHKERVYGRGREWLAKLDTIVHELYHVDPHEGGIRRGSNGDGRLSALTHT
                     PQFFREVQGIVRAYLASGPDSSVVEFLSFDFDALTRRYGGVVGTVFRGYPAFPQRYRH
                     RLKVQPRAPRAAQIVLVPSQPARRVFTEADLEKRQFLRGSTLRLGDWGQVQ"
     gene            2416..3177
                     /locus_tag="PLT35_06540"
     CDS             2416..3177
                     /locus_tag="PLT35_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28925.1"
                     /translation="MTLHARRSLTAAILAAALVGGASAHAQIGGFLKKKLPKLPVTGA
                     SAPAPAAPERPKYCAAITDEVLDRFLSALNAENAALAKRQAARQRVADAGRYAAEVMT
                     PEGFAKFEACKEAAIAKDPRTKERDRLRALADDAVRKVDNRKYQEYSAQADAIDELID
                     ASAEKACGGGPKAAAASLEAADTQAAVTALMADAEDPARAGSKAGGFTEQEYARLKEC
                     VIGRLSTPNTTPTTPESAEAIDKRAAELEKALPLK"
     gene            3174..4019
                     /locus_tag="PLT35_06545"
     CDS             3174..4019
                     /locus_tag="PLT35_06545"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOG28926.1"
                     /translation="MRVFAAGFAVVLAVVSAPGPRQTGATAVPGLAWHESAPGLERTE
                     FDLPGARGGWRTRIVAVRVDPNRFRFRLRARLSGARPAWTVDRAPESAVLAMNAGQFS
                     GLAPWGWLVMGGREIRPPGRGPLSTAVAWDAAGRVRWLAPSDIEAERAAGRTAEAIQS
                     YPTLIDERGAVPRPLREPGLGVDAEHRDARLAMGSLPDGRLAVVITRFNGFGAVSPAL
                     PLGLTLGETADVMLGLGCRRAVSLDGGLSAQLLVRERGRTLAWRGWRPVPLGLVAEPA
                     ADGGD"
     gene            complement(4025..5137)
                     /locus_tag="PLT35_06550"
     CDS             complement(4025..5137)
                     /locus_tag="PLT35_06550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HOG28927.1"
                     /translation="MKHRLLLTIAMAALAVPAARSYYLNGPSWPAGSVISMHVQLGPS
                     PRLADGCLDWACSAAAAAELWNVALGRVRLRTVQDEGVAQGRQNGRNDMFFASNAYGY
                     ALESTTLAVTTWWQRLGRITEADITFNSALQWDSYRGDLERRLHDLRRVAAHEMGHVL
                     GLGHPDEQGQTVHALMNSKIGNLDTLTVDDIQGVLALYGLSGAGVAEPIGTVAFPPRD
                     ETFEFRAWLETKYRDALGRPATLSYTDVEGSVVWVQEYLRYRLNACGHEGAVGRVRIQ
                     IAGGGIVGACGTPKPGSVIVFPSRDETHAFRRALEAIYRDDLGRAAVKTYVDAEGDVV
                     WIQEYLRYRLNGCTHAQSVEKVGRQIDGFGIQPVCR"
     gene            complement(5148..>5752)
                     /locus_tag="PLT35_06555"
     CDS             complement(5148..>5752)
                     /locus_tag="PLT35_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28928.1"
                     /translation="RPVVTSRRSIVRFVASLALAAGAAAPASASSILPQTLPELVARS
                     RTVFVGETIGVESRWADVGADRVIVTRVTFKVDRVLKGRLGGQTVLEFLGGTVGEDRL
                     AVAGMPKFRVGDRDVLFVDDGGRPMCPVVGMTHGRFRVLEDPQTGRRSMARSGFEPLA
                     AVGDIGAAGPPARVRSARALTLEAFASEIEREVERQRAAR"
BASE COUNT          828 a         2107 c         2023 g          794 t
ORIGIN      
        1 acgcggacct cgggcatgcc ggttccccgg cggtgcaatc cggatccgca tagggcaata
       61 cgaccgcccg gaacggccca cgaggggtcg gccgaggcga cgcgggctgg cgtggagtca
      121 ggcacagagc accaggcgcg gcatccccgc tactcgtcgt cgagctcgac gttccagtag
      181 aggtagtcgc gccagctctc cggcgcatag cggaacccga tggtgatccg cagcgcaggc
      241 gccgactcgg ggcgcttcgg gagccggatg agccgcatgc cggcgccggc cggcgtccgg
      301 ccgcctttct tgcggttgca ggccacgcag caggtgacga tgttctccca gtccttggtg
      361 ccgccttgcg cgaccggcac gacgtggtcg aacgtcaggt cggaggtcgg cagcctccgc
      421 ccgcagtact ggcaggtgtg ctcgtcgcgg gcgtagatgt tcgcgcggga gaacggcacg
      481 tagtcgaacc ggtgcttgat gcggacgtag cgcagcaggc ggatgaccga cggcagcttg
      541 atcgtgaagg agacgctgcg gacgtcccgg tcgtagacgg aaatcacctc gaccttgccc
      601 tggcacagca gcgtcaccgc cttcttccag tgcacgactt tgaggggctc gaacgaggcg
      661 ttgaggagga gcgtctgctc catgcgcgca ttctacaaca agacgcggcg gcgtccggct
      721 gttccccgga ggccggacgg ccgatagcct gatcgagatg gcatattgcc ggacgcgcgg
      781 ggcggcgtgc gccgcgattg cgggagcggc gctggcggcc gcatgtgccg gacagcagcc
      841 gcgcgtgcgg gtgctgccgc cgcgcgccga acccgctgca gaagcgcccg cgccgggccg
      901 gccgtcaccc gcgctgccgc ccgccgatgg cggcggggcc gcgttcgccg gcgaggcgat
      961 tgcgcggacg gcagagcgcc tgctcggcgc gccgtaccgg gacgggggcg cgcagccgga
     1021 cgggttcgat tgcagcggcc tcgtcagcta cgtcttcgcg cgccacggca tccccgtgcc
     1081 gcgcgacgtg cgccgccagg ccgtcgccgg gatcgaggtc gagccggccg aggttctgcc
     1141 cggcgacctg ctcttcttcg cgaccaccgg gtccgggccg acgcacgtcg gtatcgcgat
     1201 gggcggcggc cgcttcatcc acgcgcccaa gaacggcgcc gtggtgcgga tggagtcgct
     1261 gtcgtccacg tactggtcgt cgcgcttcgt gtcagcccgt cgtctgctgg ggtcaggtcc
     1321 agtaaaacgg ggtcaggtct cgacctgacc ccgttttact ggacctgacc ccaatccccc
     1381 aatctgagcg tcgagccgcg caggaactgc cgcttctcca ggtcggcctc ggtgaacacc
     1441 cgccgcgccg gctgggatgg caccaggacg atctgcgccg cccgaggcgc gcgcggctgc
     1501 accttgagcc ggtgccggta ccgctgcggg aacgccggat agccgcggaa cacagtcccg
     1561 acaacacctc cgtagcgccg cgtcagcgcg tcgaagtcga acgacaagaa ctccacgacc
     1621 gacgagtccg gcccgctcgc aaggtacgcg cggacgatgc cctggacctc ccggaaaaac
     1681 tgcggcgtgt gggtcaacgc cgacagtcgg ccgtcgccgt tcgagccgcg ccggattccg
     1741 ccctcgtgcg gatcgacgtg atacagctcg tgcacgatcg tgtccagctt ggcgagccac
     1801 tcgcggcccc gcccgtacac ccgctccttg tgcgtgtcgg ccgcggcctg atcgcagaaa
     1861 cgcggcagcg ccaccgagac gaggtgccag atgcgcctcg gtccgatgcg gacctcgggc
     1921 gaccgcacaa cgaaccactc tgaccgcttg gccatcgcgc cggtgcgggg atcccgccag
     1981 tagtagtacg ccggctcgct gtccgggacc agcagcgagt ggcacgtcga gtacgggccg
     2041 tgggcccccg tccgcccggg acgcgcgaag accagcaccc tgtttacgtc cacatgcgag
     2101 agcgccggca cgcgggagac gatatccgcc atcagccggt ggacgtgatc ggtatagtca
     2161 atcatgcagg gcacgcgcag gcagggcccc gtcacgggcg cccgcggcgc gtcgctgtgg
     2221 tgtgtcggcc ttgacccgaa cgggctttag cgggcccgct ggcatcgcga cgcgctggat
     2281 gccgcgtgcg ggaccggcgc cggccgtgct atcctgcaag tgggtaccct aacctgcagc
     2341 gaccagtcgc ccggctgccc tccatggccg actgcctccg aggtgcgtca gcgcgcgcga
     2401 cacccggagg ccccgatgac cctgcacgcc cggcgttcgc tcaccgccgc gatcctggcc
     2461 gccgccctcg tcggcggcgc atcggcccac gcccagatcg gcgggttcct caagaagaag
     2521 ctcccgaagc ttcccgtcac cggcgcgtcg gccccggctc cggccgcgcc cgagcggccg
     2581 aagtactgcg cggcgatcac cgacgaggtc ctcgaccgct tcctgtcggc gctcaacgcc
     2641 gagaatgcgg ccctggcgaa gcggcaggcg gccagacagc gcgtggccga cgcggggcgc
     2701 tacgccgccg aggtgatgac cccggagggg ttcgcgaagt tcgaggcctg caaggaagcg
     2761 gccatcgcga aggaccctcg gaccaaagag cgggatcgcc tccgggctct cgctgacgat
     2821 gcggtccgca aggtcgacaa ccgcaagtac caggagtact cggcgcaggc ggacgccatc
     2881 gacgagctga tcgacgcctc agccgagaag gcctgcggcg gagggccgaa ggccgccgcg
     2941 gcttcgttgg aggcggctga cacccaggca gctgttacgg ccctgatggc cgatgcggaa
     3001 gacccggcca gggcgggctc gaaggccggc ggcttcaccg aacaggagta cgccaggctc
     3061 aaggagtgcg tgatcggacg cctgtccacg ccgaacacga ccccgaccac gccggagtcg
     3121 gccgaggcca tcgacaagcg cgcggccgaa ctcgagaagg cgctgccgct caagtgagag
     3181 tgttcgctgc cggcttcgcc gtcgttctcg ccgtggtgtc ggcgccaggg cctcgccaga
     3241 ccggggcgac ggcggtgcct ggcctggcgt ggcacgagtc ggcgccgggc ctcgagcgga
     3301 cggagttcga cttgcccggc gcgcgaggcg gatggcgcac gcgcatcgtg gccgtgcgcg
     3361 tcgatccgaa ccgcttccgg ttccgcctcc gcgcgcgcct cagcggcgcc cgccccgcct
     3421 ggacggtgga tcgcgcgccg gagtcggccg tcctcgccat gaacgccggc cagttctcgg
     3481 gcctggcgcc ctggggctgg ctcgtcatgg gcgggcgcga gatccggccg cccggccggg
     3541 gcccgctttc caccgcagtg gcgtgggacg cggccggccg cgtccgctgg ctggccccct
     3601 ccgacatcga agccgagcgt gcagccggac gcacggcgga agccatccag tcgtacccga
     3661 ctctcatcga cgagcggggc gcggtccctc gcccgctccg ggagccgggc ctcggcgttg
     3721 acgcggaaca tcgcgatgcg cgcctcgcga tgggctcgct ccctgacggc cgcctggccg
     3781 tcgtcatcac ccgcttcaat ggcttcggcg cggtgagccc cgcgcttccc ctcgggctca
     3841 cgctcggcga gacggccgac gtcatgctcg ggctcggctg ccggcgcgcc gtgtcactcg
     3901 acggcggact gtcggcgcag ctgctcgtgc gggaacgcgg tcgcaccctg gcctggcgcg
     3961 gctggcggcc ggtgccgctc ggcctggtcg cggagccggc cgccgacgga ggcgactgag
     4021 gcgcctatct gcacaccggc tggatgccaa acccgtcgat ctgccggccc accttctcga
     4081 ccgactgcgc gtgcgtgcag ccgttcagcc ggtaccggag gtactcctgg atccagacga
     4141 cgtcgccctc ggcgtccacg taggtcttca cggccgcgcg cccgaggtcg tccctgtaga
     4201 tcgcctcgag cgcccgccgg aacgcgtggg tctcgtcgcg cgacgggaac acgatgacgc
     4261 tgcccggctt cggcgttccg caggcgccca cgatcccgcc gccggcaatc tggatccgca
     4321 cccgcccaac cgccccctcg tggccgcacg cgttgagccg gtacctcagg tactcctgca
     4381 cccacacgac cgagccctcg acgtccgtgt aggacaacgt ggcgggccgg ccgagcgcgt
     4441 cgcggtactt ggtctccagc cacgcccgga actcgaacgt ctcgtcgcgc ggcgggaacg
     4501 ccaccgtgcc gatcggctcg gcgacgccgg cgcccgacag cccgtagagc gccagcacgc
     4561 cctggatgtc gtccaccgtc agcgtgtcga ggttgccgat cttgctgttc atcagcgcgt
     4621 ggacggtctg tccctgctcg tccggatggc cgaggccgag cacgtggccc atctcgtgcg
     4681 cggcgacgcg ccgcaggtcg tgcaggcgcc gctcgaggtc cccgcggtag gagtcccatt
     4741 ggagcgccga gttgaacgtg atgtcggcct cggtgatccg ccccagccgc tgccaccacg
     4801 tcgtcaccgc cagcgtggtg gactccagcg cgtagccgta cgcgttgctg gcgaagaaca
     4861 tgtcgttgcg gccgttctgc ctgccctgcg cgacgccctc atcctgaacg gtccgcagcc
     4921 gcacgcgccc cagggcgacg ttccacagct ccgccgcggc ggccgccgag cacgcccagt
     4981 ccaggcagcc gtcagccagc ctgggagacg gcccgagctg gacgtgcatg gagatgaccg
     5041 agcccgcggg ccacgacggg ccgttcaggt agtaggaccg cgccgccggc acggccagcg
     5101 ctgccatcgc gatcgtgagc aggagcctgt gcttcatggc ggactcccta tctggcagcg
     5161 cgctgccgct cgacctcgcg ctcgatctcg ctggcgaacg cctcgagcgt gagggcgcgg
     5221 gccgacctca cgcgcgcggg cggcccggcg gcgccgatgt caccgacggc tgcgagcggc
     5281 tcgaagcccg atctcgccat cgatcggcgg ccggtctgcg ggtcctccag aacccggaac
     5341 cggccgtgcg tcatgcccac gaccggacac atcggacggc ccccgtcgtc gacgaacaag
     5401 acgtcccgat ccccgacgcg gaacttcggc atgccggcca cggcgagacg gtcctccccg
     5461 accgtgccgc cgaggaactc aaggacggtc tggccgccca gccggccctt cagtacgcga
     5521 tccaccttga aggtgacgcg cgtgacgatc accctgtctg cgccgacgtc ggcccatcgc
     5581 gactcgacgc cgatcgtctc gccgacaaag accgtccgcg agcgcgcgac gagttcgggg
     5641 agcgtctgcg gcaggatcga ggacgccgag gccggggccg cagctcccgc cgcgagcgcg
     5701 agcgaggcca cgaaccgcac aatcgatcga cggcttgtca cgacgggcct cc
//
LOCUS       DAOSIF010000154         5727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12668_length_5727_cov_4.883286, whole genome shotgun sequence.
ACCESSION   DAOSIF010000154 DAOSIF010000000
VERSION     DAOSIF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12668_length_5727_cov_4.883286"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1108)
                     /locus_tag="PLT35_06560"
     CDS             complement(251..1108)
                     /locus_tag="PLT35_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOG28929.1"
                     /translation="MRTHLTRRDVLKLGGVAACAALAGRPDLLRAAAGWKKIPIGVQL
                     WCVRKQLATDISGVLGALAAAGFDGVELENAFKTPGPEWRKHLDSAKLKACGFHHTLA
                     ELQGEKLAATVEFNQAIGNPYLIIRSLPADVYTSADLLKKTADAVNEAAAKVKPHGMC
                     VGYHNHFADFNRIGGEYWWNLFADATTKDVVLQLDTGNAAELPGVPLADLVRRNAGRT
                     VTMHIKPYSKANPNAYIGADELDWPAIMAAAESVGGLDWYIVEYEREGTPPVEALKAN
                     LDGFRKLRG"
     gene            1372..2292
                     /locus_tag="PLT35_06565"
     CDS             1372..2292
                     /locus_tag="PLT35_06565"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOG28930.1"
                     /translation="MDEPDARLEEELRRGAQYLAQRLTPTAGAIPRLPGIGIHGVSLP
                     LNGIAGGDLLTYVDFRARYDLDARIARALAAGQERLAWVLQKLKHVGGILVADVAGHD
                     FTDAARAAMLHQAFHTGALYEMDLTGQITLRLFEQINTRFCKSTTLRSLAADPDEASY
                     ITLIYGEISSTGRFSFLSAGHPPPLVFSREFDRFVEVSQDRLVSYPPIGLQPAAHHVD
                     ARRYERPLGYKKRYTVNNLNLMGQGDVLLLYTDGLIEPDSAYTQAHLEQAVSRAKDGS
                     AQAICEAIISDRQAATPQTDDLTLVVIKYR"
     gene            complement(2453..2629)
                     /locus_tag="PLT35_06570"
     CDS             complement(2453..2629)
                     /locus_tag="PLT35_06570"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOG28931.1"
                     /translation="MVAESTKRRGYSPVPTLPALAGRIVDSADVSDRFARDVRRTLAD
                     MWADSHYGTMADLL"
     gene            complement(2882..4015)
                     /locus_tag="PLT35_06575"
     CDS             complement(2882..4015)
                     /locus_tag="PLT35_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOG28932.1"
                     /translation="MNIRFDRVERAQQLMREQGWLGIMVMNHDDYRYFFGADRSQPRA
                     IIPFEGPPVLVAFAGEEPELRDATAGAPIRVFTHVGGQIQDVVMAFRDLMKSRGGPPP
                     GAKPRVGMQMWFETPAFLVDLFRRVNPALELVPSDPVMDRLRMVKEPEEIALLERAQS
                     IAALGMDRVREMIAPGASPHEIATEALYAMMKAGAERTSTPIYVNAGRDTCWLHGRIS
                     AGPIREGDLVLVDLTPQFEGYCANLARTFVVGRPDGEQQRLLDAYAEMREASRDKLRP
                     GTKVADIDARGLEVCASHGLGEAHVDGISHGIGLRFEETPASTIVKPHRNVALLEGMT
                     VTVGHTILALPGLGGVRHEDIYEVTPNGGRILMPYPVDPVVGR"
     gene            complement(4248..5663)
                     /locus_tag="PLT35_06580"
     CDS             complement(4248..5663)
                     /locus_tag="PLT35_06580"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HOG28933.1"
                     /translation="MDRNHTVVPAAGALETRIEEWRAHLRRSRAISSTDSAELEDHLR
                     EDIASLRAGGLSDDEAFLVGVRRLGAIDALTREFAREHSDRLWKQLVLSGDGGDGGIG
                     SSASGRVRKMWVALGLAAAAAAAIKLPALFGISFDAEPAAAFYPRNIGFFTLPFIAAY
                     FAWDRPLERTGRFGLAAAFAAAAVVVNLFPFSVSPKGGPPHTLILTMLHLPIALWLGV
                     GVAYAGGRWRGSDRRMDFVRFTGEFFIYYVLIALGGGVLMALSAGIFGAIGVGMEPLL
                     ESWVLPCGAAGATVVAAWLVEAKQRVVENMAPVLTRIFAPLFALLLIAFVVTMAATGR
                     AIDAKREVLILFDLILAVVFGLLLYSASARDAAAPPGPGDWIQLTLVGAALVVDGLAL
                     RAIGSRIFEFGFTPNRVAALGENIVLLVNLGGSAVLYSGFLRGRAAFSRLCDWQQFYL
                     YILAAWAAIVALLFPLAFGFE"
BASE COUNT          838 a         2079 c         2002 g          808 t
ORIGIN      
        1 gccgagaagg cgccgacggc cgtgccggat ccccggacgt tgcgcggcgc ggcgtttctc
       61 atcgccgccg ccgccctcga gcaactgcct ccgcccggcc cgcccgaggt ggcgttcgcc
      121 ggacggtcga acgcggggaa gtcgagcgcg aaggagcgcg cggccatggt cgaataatta
      181 ttcgactaca ataggctctg tcaaccacgc acgcccggcg gcgcgcctcc cgcgctcgcc
      241 cgggcggccg tcagccgcgg agcttcctga agccgtcgag gttcgccttc agcgcctcga
      301 ccggcggcgt gccctctcgc tcgtactcga cgatgtacca gtcgaggccg cctaccgact
      361 ccgcggccgc catgatggcg ggccagtcga gctcgtcggc gccgatgtaa gcgttcgggt
      421 tcgccttcga gtacggcttg atgtgcatgg tcacggtccg cccggcgttg cggcggacga
      481 gatccgcgag cggcaccccc ggcagctccg cggcgttgcc cgtgtcgagc tgcaggacga
      541 cgtccttcgt ggtggcgtcg gcgaacaggt tccaccagta ctcgccgccg atgcggttga
      601 agtcggcgaa gtgattgtgg tacccgacgc acatcccgtg cggcttcacc ttcgcagccg
      661 cctcgttcac ggcgtccgcg gtcttcttca ggaggtcggc cgacgtgtag acgtcggccg
      721 ggagcgacct gatgatcagg taggggttgc cgatggcctg gttgaactcg acggtcgcgg
      781 cgagcttctc gccctgcagc tcggccagcg tatggtggaa gccgcacgcc ttcagcttcg
      841 cgctgtcgag gtgcttccgc cactccggcc cgggcgtctt gaaggcgttc tcgagctcga
      901 cgccgtcgaa cccggccgcc gccagggcgc caagcactcc ggaaatgtcc gtggcgagct
      961 gcttccggac gcaccagagc tgcacgccga tcgggatctt cttccacccg gccgcggccc
     1021 gcaggagatc cggccggcca gccagggcgg cacaggcggc aacgccgccg agcttgagga
     1081 cgtcgcggcg agtcaggtgc gtgcgcatga gggaagtcct ttcgatggcg cagatcctat
     1141 gccggtcccg gcggagcggg caagcggcgg cgcctgaggc gcggctcgcg gcggcgctgc
     1201 tcgagcggcg gccgcgacgc cgccggccgg aagccgaccg gacgccgggc atgctgccgc
     1261 ccgagcggag ccgccccgtg ccgccgcaac cgccatggcc gcgcgtcgcg cggacgcgag
     1321 aagggggata atcgttcgtc tggacgaggc gcggggacgc gggaggcgat tgtggatgaa
     1381 ccggatgccc ggctcgagga ggaacttcgc cgtggcgcgc agtacctggc gcaacgcctg
     1441 acgccgacgg ccggggcgat ccccaggctg ccgggcatcg gcatccacgg cgtgtcgctg
     1501 ccgctcaacg gcatcgccgg aggcgatctc ctcacctacg tcgatttccg cgcacgctac
     1561 gacctggacg cccggatcgc ccgcgcgctg gcggccgggc aggagcgcct cgcgtgggtg
     1621 ctccagaagc tcaagcacgt cggcggcatc ctggtcgccg acgtcgccgg ccacgatttc
     1681 accgacgcgg cgcgcgcggc gatgctgcac caggccttcc acaccggcgc gctctacgag
     1741 atggacctga ccggccagat cacgctgcgc ctcttcgagc agatcaacac gcgcttctgc
     1801 aagtccacga cgctgcgcag cctggccgcc gaccccgacg aggcgagcta catcacgctc
     1861 atctacggag agatctcctc gacgggccgc ttcagcttcc tgagcgcggg gcacccgccg
     1921 ccgctcgtct tctcgcgcga gttcgatcgc ttcgtcgagg tgagccagga tcgcctcgtc
     1981 agctacccgc cgatcgggct gcagccggcc gcgcaccacg tcgacgcgag gcgctacgag
     2041 cggccgctgg gctacaagaa gcgctatacc gtcaacaacc tcaacctcat gggccagggc
     2101 gacgtgctgc tgctctacac cgacggcctc atcgagccgg attccgctta cacccaggcc
     2161 cacctcgagc aggcggtctc gcgggccaag gacgggagcg cgcaggcgat ctgcgaggcc
     2221 atcatcagcg atcgccaggc ggcgacgccg cagaccgacg acctcacgct cgtcgtcatc
     2281 aagtaccggt aagctgcgcc gcggacgtcc tgcaggtcca tcagccgcgg ctgcagctca
     2341 cgcacccgga actcgccggc gggaatcccg agcttgctct tgttgagcag cgtgtcctcg
     2401 ggcatctcca gcgacaggcc ggcggcccgc cgtagatgcc gacgccgtcc tttcacagca
     2461 ggtcggccat cgtgccgtag tggctgtcgg cccacatgtc ggcgagggtc cggcggacgt
     2521 cccgcgcgaa ccggtcgctg acgtcggcgg agtcgacgat cctgcccgcg agcgcgggca
     2581 gggtggggac cgggctgtac ccgcggcgct tcgtggactc cgcgaccatc ccttcggtcc
     2641 aacggcccgc ccgtctcgct ccagccaagc gagctgagga tggccatttc gaggccgagg
     2701 cggtccgctt cggccgcggc atggcggacc gcgtcgggcc actccggcgt gccgaagggg
     2761 atcctgggct cgaccgtccg gcctccgccc gagttgacgc cggcgcggcc gcgcgcccgc
     2821 ggccgcgcgg gcgatgacgg tggcggagcg gccgcgcggt ctcgtgggcc cgcgggcccg
     2881 atcagcgccc gacgaccgga tcgaccggat acggcatcag gatccggccg ccgttcggcg
     2941 tcacctcgta gatgtcttcg tgccgcacgc cgccgaggcc cggcagggcc aggatggtgt
     3001 ggccgacggt gaccgtcatg ccctccagca gcgccacgtt cctgtgcggc ttcacgatcg
     3061 tcgaggcggg cgtctcctcg aagcgcaggc cgatcccgtg cgagatgccg tcgacgtgcg
     3121 cctcgcccag gccgtgcgac gcgcagacct cgaggccccg cgcgtcgatg tcggccacct
     3181 tcgtcccggg ccggagcttg tctcttgacg cctctcgcat ctcggcatag gcgtcaagca
     3241 gccgctgctg ctccccgtcg gggcggccga cgacgaaggt gcgcgcgagg ttggcgcagt
     3301 agccctcgaa ctgcggcgtc aggtcgacga ggaccagatc gccctcccgg atcgggcccg
     3361 cgctgatccg tccgtgcagc cagcacgtgt cccggcccgc attcacgtag atcggcgtgc
     3421 tcgtgcgttc cgcgccggct ttcatcatcg cgtagagcgc ctcggtggcg atctcgtgcg
     3481 gcgaggcgcc gggcgcgatc atctcgcgca cccggtccat gccgagcgcc gcgatggact
     3541 gcgcccgctc cagcagggcg atctcctccg gctccttcac catccgcagg cggtccatca
     3601 ccgggtccga cggcacgagt tccagcgcgg gattgacgcg cctgaacaga tcgaccagga
     3661 acgccggcgt ctcgaaccac atctgcatgc cgactcgcgg cttcgcgccc ggcgggggcc
     3721 cgcccctcga cttcatcagg tcccggaacg ccatcacgac gtcctggatc tgtccgccga
     3781 cgtgggtgaa cacgcggatc ggggcgccgg ccgtggcgtc ccggagttcc ggctcttctc
     3841 cggcgaacgc cacgagcacg ggcgggccct cgaaggggat gatggcccgc ggctgggacc
     3901 ggtctgctcc gaagaagtac cggtagtcgt cgtggttcat caccatgatg cccagccatc
     3961 cctgttcgcg catcagctgc tgggcccgct ccacgcggtc gaaccggatg ttcatgctcg
     4021 ttcctcctgc tgccgccgcc cgtccgcccc agcgtagacc gcccgccggg ctccgggcgg
     4081 ccggctgcgc cccgggcggg cttctcccgt cgcctctctt cgtattctct caggctgagg
     4141 cccgccgcgc gcaggttccg atccgcgggg ccgggcggat ggcgccgcag ggcgacacgc
     4201 cggcgcgcgc ggcgccggct gtccgcgctc ccctgctctt cggccgctca ctcgaatcca
     4261 aacgccagcg ggaacaggag cgcgacgatg gccgcccagg cggccaggat gtagaggtag
     4321 aactgctgcc agtcgcacag gcgcgagaaa gcggccctgc ctcgcaggaa acctgagtag
     4381 aggacggccg agccgcccag gttgaccagg aggacgatgt tctcgccgag ggccgcgacg
     4441 cggttgggcg tgaagccgaa ctcgaagatc cggcttccga tggcccggag cgcgagcccg
     4501 tccacgacca gggcggcacc gaccagcgtc agctgaatcc agtcgccggg cccggggggc
     4561 gcggccgcat cgcgggctga cgccgagtag aggagcagcc cgaagaccac ggccaggatg
     4621 aggtcgaaga gaatcaggac ctcccgcttc gcgtcgatcg cccgcccggt cgccgccatg
     4681 gtcacgacga aggcaatgag cagcagggcg aagagcggcg cgaagatgcg cgtcagcacg
     4741 ggcgccatgt tctcgacgac gcgttgcttc gcctcgacga gccaggccgc gacgaccgtg
     4801 gccccggcgg cgccgcacgg caggacccag ctctccagga gcggctccat cccgacgccg
     4861 atggccccga agatgccggc ggacagcgcc atgaggacgc cgccgcccag cgcgatgagg
     4921 acgtagtaga tgaagaactc gccggtgaag cggacaaagt ccatccgccg gtcgctgccc
     4981 cgccagcgcc cgccggcgta ggccacgccc acgcccagcc acagggcgat gggcaggtgc
     5041 agcatcgtca ggatgagggt gtgcggcggt ccgcccttcg gtgagacgga gaacggaaag
     5101 aggttcacca cgaccgcggc ggcggcgaag gcggcggcca gcccgaaccg gcccgttcgc
     5161 tcgaggggcc ggtcccaggc gaagtaggcg gcgatgaagg gaagcgtgaa gaagccgatg
     5221 ttgcgcgggt agaacgcggc ggcgggctcc gcgtcgaacg agatcccgaa cagggcgggc
     5281 agcttgatcg ccgcggcggc cgccgcggcc aggccgagcg ccacccacat cttccggacc
     5341 cggccggagg cggacgagcc gatgccgccg tccccgccgt cgccggagag cacgagctgc
     5401 ttccacaggc gatccgagtg ctcgcgcgcg aactcccgcg tcagggcgtc gatcgccccc
     5461 agccgcctga cccccaccag gaaggcctcg tcgtccgaga ggccgccggc gcgcagcgac
     5521 gcgatgtcct cgcgcaggtg atcttccagc tcggccgagt ccgtgcttga gatggcgcgg
     5581 ctccgccgga ggtgggcccg ccattcctcg atccgggtct cgagcgcacc ggccgccggc
     5641 acgaccgtgt ggttccgatc catgggctca gctccgcagc ttctcgagcg tcttgtgcac
     5701 ggtctgccat tggcgccgct cgtcggc
//
LOCUS       DAOSIF010000155         5718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12694_length_5718_cov_3.076285, whole genome shotgun sequence.
ACCESSION   DAOSIF010000155 DAOSIF010000000
VERSION     DAOSIF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12694_length_5718_cov_3.076285"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PLT35_06585"
     CDS             complement(<1..1144)
                     /locus_tag="PLT35_06585"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG28934.1"
                     /translation="MPTTGLSIPFRSRLLDNGLRVIVHVDRHCPVAAVNVWYHVGSKD
                     DPPGRSGLAHLFEHLMFKGSAHHDASYFEPLQQAGGVLNGSTNADRTNYWEVVPSNAL
                     ELALWMESDRMGFLLPALTGARFAAERDVVLNERRQRYENRPYGLAPIAMAAALYPAG
                     HPYCRATIGSPEDLCGATLDDARAFFSRFYHPANASLAIAGDVEADEAFDLADAHFGE
                     IPRGNAPPVVPLAPPAASPSRLLLEDRVELPRLYLGWLSPAAYAAGDAELDLLAAALA
                     GGKSSLLHRSLVYERRLATDVLAAQVSRERGGAFQVAATAAPGVPLDTLERAIDDELD
                     RVRAAGLTGDELARALAGAESAFISRLQAVGGFGGKSDQLNHYSVML"
     gene            1309..1512
                     /locus_tag="PLT35_06590"
     CDS             1309..1512
                     /locus_tag="PLT35_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28935.1"
                     /translation="MSVGRFSLSLFGTIAVLSAVLAGATIWLLITDPVTVADAVESGH
                     VSPLVEALVGVLFDALKGLLRYL"
     gene            complement(1544..2905)
                     /locus_tag="PLT35_06595"
     CDS             complement(1544..2905)
                     /locus_tag="PLT35_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOG28936.1"
                     /translation="MTAPLHVRTIEELAPLVAARRVSSEELTRACLAEIDARDASLRA
                     FITVTADTALAEARRCDEEAAGGFVRGPLHGMPVSLKDVIDQRGVPTTAASRVRDGHV
                     ARQDAPAAARLRAAGAVLIGKTNLHEFAFGTTSDETAYGAVRNPYDPARSPGGSSGGS
                     AVAVVTGMSVASIGTDTGGSIRIPASACGVVGLKPAHGDVPTAGVVPLSWSLDHVGPL
                     ARSVADAWLLYLVLRGDAALARWPLPSRRSIKGLRLGVPRAYFHDVLDDEVRQRFAAS
                     LARLRHAGALSADVALPRAPLGPAAYALLSLAEAAAYHAASLGSEPGRYTPKVRLRIE
                     CGRYVLAEDYVRASRARDVLRRDVDAALAGCDALALPTLPIPAPPIGAETVEIGGAPR
                     PVRQMMLRLTQLFNLTGHPAISLPAGRTSAGLPCGFQLVGRPGDTEGLLAIASACEPH
                     VAA"
     gene            complement(2902..3147)
                     /locus_tag="PLT35_06600"
     CDS             complement(2902..3147)
                     /locus_tag="PLT35_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28937.1"
                     /translation="MTVQDWYAWACADAERRGVPALKPLLQALADWTQALRSADWDAA
                     ERPSSADPAAPSGGRAPEGPAAEGDSGATGPTDGARS"
     gene            3353..4951
                     /locus_tag="PLT35_06605"
     CDS             3353..4951
                     /locus_tag="PLT35_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG28938.1"
                     /translation="MRSALPTPMMAEQPSDARPETVAAIDVGASAVRLVIAELTAGRP
                     PEVIDEAIRGVSLGKETFFAGRIGSEPMDAVLRALKGFARLMGEHGVTRYRAVATSAV
                     REASNADTFLDRVLVKTGLRLEVIDLAEESRLVYLAVRNGLAGHPALAARHALIVEVG
                     GGSADITLLAGARPKYSGVYALGSVRLRQALRPWASDAERRVRLLTRNIGGVVRAISR
                     DVPLRSAEFMIGLGGDMRLAAREVAGGGEGAVCEVPREPFLQFCAQVERFGEEALAER
                     FGLTLVDAETLVPALLVYRALLLETPAQGITVPPVSLRDGLLADMAAPPSGSAADGLA
                     EFSQQVLAGAEALGEKYRHDAAHARHVALLATRLFDELRDEHGLEPRDRLLLEVAALL
                     HNIGSFVSLRAHHKHAQYLIQASEIFGLSAGDRAIIANVARYHRRGLPQPSHVPYMAL
                     DRTERVRVNKLAAILRVANALDAENAQKVREVRIVRADGALCLDVTGVGDLAMERERV
                     QARANLFQDVFGCVLAWRGTGVQP"
     gene            4948..>5718
                     /locus_tag="PLT35_06610"
     CDS             4948..>5718
                     /locus_tag="PLT35_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28939.1"
                     /translation="MSARRTAGAKRRASAGAGRPGAYFNRELSWLAFDERVLEEAADA
                     SNPLLERVKFAAIVASNLDEYFMVRVARLVHAIEEGDDEPDPSGLSPARQLALVSDRV
                     HALVHQLYRQVNEHLIPALARHGIRIVGPQDLDEPGRAAVAAYFRDDVLPVLTPMAVD
                     TARPFPMLSSLSVNLALRLAPAGEGLPERIAVVQVPSVLPRLLRVAGGEGVTFVSLDA
                     LIRDGLAALFPGQAIVECAAFRLTRDAELELDDEGGRSY"
BASE COUNT          735 a         2073 c         2142 g          768 t
ORIGIN      
        1 cgagcatgac gctgtagtga ttcagctggt cggacttgcc gccgaagccg ccgacggcct
       61 ggagccgtga gatgaacgcg gactccgcgc ccgctagcgc ccgggccagc tcgtccccgg
      121 tcaggccggc cgccctgacg cgatcgagtt cgtcgtcgat ggcccgctcg agcgtgtcga
      181 gcggcacgcc cggcgccgcc gtcgccgcca cctggaacgc gccgccgcgc tcgcgcgaca
      241 cctgggccgc gaggacgtcc gtcgccagcc ggcgttcgta gacgagcgag cggtggagca
      301 gggacgactt cccgcccgcc agcgcggcgg ccagcagatc gagttccgcg tcgccggcgg
      361 cgtacgctgc ggggctcagc cacccgaggt agagacgagg cagctcgacg cgatcctcga
      421 gcagcagcct cgacggggag gccgccggcg gcgccagcgg cacgacgggc ggcgcgttcc
      481 cgcgcgggat ctccccgaag tgcgcatctg cgagatcgaa ggcctcgtcg gcctccacgt
      541 cgccggcgat ggcgagcgat gcgttggcgg ggtggtagaa gcggctgaag aacgcccgcg
      601 catcgtcgag cgtggccccg cacaggtcct caggcgaccc aatcgtggcc cggcagtagg
      661 gatggccggc ggggtagagc gcggcggcca tggcgatggg cgccaggccg tacggccggt
      721 tctcgtagcg ctgccggcgc tcgttcagca cgacgtcgcg ctcggccgca aagcgggcgc
      781 cggtcagcgc cggcagaagg aagcccatgc ggtcggactc catccacagc gcgagctcga
      841 gggcgttcga gggcacgacc tcccagtagt tcgtgcggtc ggcgttggtc gatccgttga
      901 gcacgccgcc cgcctgctgg aggggctcga agtagctggc atcgtgatgg gccgacccct
      961 tgaacatgag gtgctcgaag aggtgggcca gcccgcttcg gcccggcgga tcgtccttgg
     1021 atcccacgtg gtaccagacg ttcacggccg cgacgggaca gtggcggtcc acgtggacga
     1081 tgacccgcag gccgttgtcg agcaggcggg agcggaacgg gatggacagt ccggtcgtcg
     1141 gcacggcgct tcgcgccggc cctggccggc ggctccgcgc atcctactac acccggcgtg
     1201 ccggccaggg gccggccgct ggcgaggtgc aaccccggcg ccggcgcgtg cgtaattctc
     1261 atcgtgcgcg ccgggctggg tgtagactag cccggtacag cctggcccat gagcgttggc
     1321 cgtttcagtc tgagcctgtt cggcacgatt gcggtgctgt cggccgtgct ggcgggcgcg
     1381 acgatctggc tgctgatcac cgacccggtg acggttgccg acgccgtcga gagcggccac
     1441 gtgtccccgc tggtcgaggc gctcgtcggc gtgcttttcg acgcgctcaa ggggctgctc
     1501 aggtacctct gaatccctcc caggccgtca gccggccccc gggctacgcc gccacatgcg
     1561 gctcgcaggc cgaggcaatc gcgagcaggc cctcggtgtc tcccggccgg cccacgagct
     1621 gaaacccgca cggcagcccg gcggaggtcc gtcccgcagg gagcgagatg gccgggtgcc
     1681 cggtgaggtt gaaaagctgg gtgagccgca gcatcatctg gcgcaccggc cgcggcgctc
     1741 cgccaatctc gaccgtctcg gcgccgatcg gcggcgccgg gatgggcagc gtcgggagcg
     1801 ccagcgcgtc gcagccggcc agcgcggcgt caacgtcgcg ccgcagcacg tcgcgcgcgc
     1861 gggacgcgcg cacgtagtcc tccgccagca cgtagcggcc gcactcgatg cgcaggcgga
     1921 ccttcggcgt gtagcgcccc ggctccgacc cgagcgaggc cgcgtggtac gccgccgctt
     1981 cggccagcga cagcagcgcg taggcggccg gccccagcgg cgcgcgggga agggccacgt
     2041 cggcgctcag cgctccggcg tgccgcagcc gcgccagcga cgccgcgaac cgctgcctga
     2101 cctcgtcgtc gagaacgtcg tggaagtacg cgcgcgggac gccgaggcgc aggcccttga
     2161 tcgaccggcg cgaaggcagg ggccagcgcg ccagcgccgc gtctccgcga agcacgaggt
     2221 agagcagcca ggcgtcggcg acggagcgag ccagcggccc gacgtgatcg agcgaccagc
     2281 tcagcgggac gacgccggcc gtgggcacgt cgccgtgcgc gggcttgagg cccaccacgc
     2341 cgcacgcaga cgccggaatg cggatcgagc cgccggtgtc cgtgccgatc gaggccaccg
     2401 acatgcccgt gaccacggcg acggccgagc ctccgctgga gccgccgggc gatctcgccg
     2461 ggtcgtacgg gtttcgcacg gcgccgtagg cggtctcatc gctggtcgta ccgaaggcga
     2521 actcgtggag gttggtcttg ccgatgagca ccgcgcccgc ggcccgcagt ctcgcggcgg
     2581 ccggggcgtc ctgcctggcc acgtggccgt cgcggacgcg cgaggccgcc gtggtcggaa
     2641 cgccgcgctg atcgatcacg tccttcaggg acaccggcat cccgtgcagc ggcccgcgca
     2701 cgaacccgcc ggcggcttcc tcgtcgcagc gcctggcctc ggcgagcgcc gtgtcggcgg
     2761 tcacggtgat gaacgccctg agcgacgcgt cgcgcgcgtc gatctcggcc aggcacgcgc
     2821 gggtcagctc ctcggacgac acccgccgcg cggcgacgag gggcgccagt tcttcgatcg
     2881 tgcggacgtg cagcggcgcc gtcacgagcg cgctccgtcg gtcgggcctg tcgcgccgga
     2941 gtcgccctcg gcggccgggc cctcgggtgc ccgcccgccg ctcggggccg ccggatcggc
     3001 ggaagacggc cgctcggcgg cgtcccagtc cgccgaacgc agcgcctgcg tccaatcggc
     3061 cagggcctgc agcaggggct tcagcgcggg cacccctcgg cgctcggcat cggcgcaggc
     3121 ccaggcgtac cagtcctgga cggtcatcgc gtgaggcctc ctcggccgcc gatcttaccg
     3181 cggcttccgg cgtgggagcc ggccgttccg ccggcccgtc ccgagcgggc acacgcccac
     3241 gagccatccc atcccccggg cccgcccgcg cagggactcc cgagcccccg gccgcgcctc
     3301 ttgggtcccg agttccgggt tcgcagcccg ccttgcctcc gggggcgata agatgaggag
     3361 cgcccttccg acccccatga tggccgaaca gcctagcgac gcgcgccccg aaacggtagc
     3421 cgccatcgac gtcggcgcga gcgcggtccg cctcgtcatc gccgagctga cggcgggcag
     3481 gcccccggag gtgatcgacg aggcgatccg cggcgtgtcg ctgggcaagg agacgttctt
     3541 cgccggccgg attggatccg agccgatgga cgcggtgctt cgcgccctga aggggttcgc
     3601 gcgcctgatg ggcgagcacg gcgtgacgcg gtaccgggcg gtcgcgacaa gcgcggtccg
     3661 cgaggcatcg aacgccgaca cctttctcga ccgcgtcctg gtgaaaacgg gcctgaggct
     3721 cgaggtcatc gatctggcgg aagagagccg cctcgtctac cttgccgtca ggaacggcct
     3781 tgccggccat cccgcgctcg cggcgcgcca tgcgctgatt gtcgaggtcg gaggcggatc
     3841 ggcggacatc acgctgctgg ccggggcgcg gcccaagtac tccggcgtct acgcgctcgg
     3901 atcggtccgg ctccggcagg cgctccggcc gtgggcgtcc gacgccgagc ggcgcgtgcg
     3961 gctgctcacc cggaacatcg gcggcgtcgt ccgcgccatc tcccgtgacg tcccgttgcg
     4021 gtcggccgag ttcatgatcg gcctgggcgg cgacatgcgc ctggcggcgc gcgaggtggc
     4081 cggcggcggc gagggcgccg tgtgcgaggt gccgcgggag cccttcctcc agttctgcgc
     4141 gcaggtcgag cgcttcgggg aggaggccct cgccgagcgt ttcggcctga cgctggtgga
     4201 tgccgagacg ctggttccgg ccctgctcgt ctaccgcgcg ctgctgctcg agacgccggc
     4261 gcaggggatc accgtgccgc ccgtgtcgct gcgggacggg ctgcttgcgg acatggccgc
     4321 gccgccctcc ggatcggcgg ccgacggcct ggcggagttc agtcagcagg tgctcgccgg
     4381 ggcggaggcg ctcggcgaga agtaccggca cgatgccgcg cacgcacgcc acgtggcgct
     4441 gctcgcgacg cgcctgttcg acgagctgcg ggacgagcac gggctggagc cgcgcgaccg
     4501 cctcctgctc gaggtcgccg cgctgctgca caacatcggg tccttcgtga gcctgcgcgc
     4561 gcaccacaag cacgcgcagt acctcatcca ggcgtccgag atcttcgggc tgtccgccgg
     4621 cgatcgcgcc atcatcgcca acgtcgccag gtaccaccgg cgcggcctgc cccagccgtc
     4681 gcacgtgccg tacatggccc tggaccgcac ggagcgcgtc cgggtgaaca agctcgcggc
     4741 gatcctgcgc gtcgccaacg cgctggatgc ggagaacgcg cagaaggtcc gggaggtccg
     4801 catcgtccgc gccgacggtg cgctgtgtct cgacgtgacc ggcgtcggcg acctcgcgat
     4861 ggagcgggag cgcgtgcagg cccgagcgaa cctgttccag gacgtgttcg gctgcgtcct
     4921 ggcgtggcgc gggacagggg tgcagccgtg agcgcgcgcc ggacggccgg ggcgaagcgg
     4981 cgcgcgtccg cgggggcggg ccggcccggc gcgtacttca accgcgagct gtcgtggctg
     5041 gccttcgacg agcgggtgct ggaagaggcc gccgacgcct cgaatccgct cctggagcgc
     5101 gtgaagttcg ccgccatcgt cgcgtcgaac ctcgacgagt acttcatggt gcgcgttgcg
     5161 cggctcgtcc acgccatcga ggagggcgac gacgagccgg atccgtcggg cctgtcgccg
     5221 gcccggcagc tggccctcgt gtcggaccgc gtccacgccc tggtgcacca gctgtaccgg
     5281 caggtcaacg agcatctcat cccggcgctc gcgcgtcacg gcatccggat cgtcggcccg
     5341 caggacctcg acgagcctgg gcgcgccgcc gtcgcggcgt acttccgcga cgacgtgctg
     5401 ccggtgctga cgccgatggc ggtggacacg gcccggccct tcccgatgct gtcctcgctg
     5461 agcgtgaacc tcgcgctgcg cctcgcgccg gccggggagg ggctgccgga gcgcatcgcc
     5521 gtcgtgcagg tcccgtcggt cctgccgcgc ctcctgcgcg tggcgggcgg cgagggcgtg
     5581 accttcgtca gcctcgacgc gctcatccgc gacgggctgg ccgccctctt ccccggccag
     5641 gcgatcgtcg agtgcgcggc gttcaggctc acgcgggacg ccgagctcga gctcgatgac
     5701 gaaggcgggc ggtcgtac
//
LOCUS       DAOSIF010000156         5707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12714_length_5707_cov_4.973992, whole genome shotgun sequence.
ACCESSION   DAOSIF010000156 DAOSIF010000000
VERSION     DAOSIF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12714_length_5707_cov_4.973992"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLT35_06615"
     CDS             <1..859
                     /locus_tag="PLT35_06615"
                     /inference="COORDINATES: protein motif:HMM:NF021895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28940.1"
                     /translation="VVLSFDPDAAGQGAAARSCDLLVAEGFQVNVAVLPGGDDPDAFI
                     RRHSGRQYAAQLQQSTPYLDYLLERTASAHDVRRDDSRREFLQAMLGVAARIPDAAAR
                     DQFADRIAHRARIAESVVRDEIRKAAVERRTEIGPREMPALGQIKPAERGLIWAAMRE
                     PDEAADALAALDEEDLEGLAAASILGQALRWQASEGIRSPAALLARLTREEAQIVTGI
                     ASAPASPAPAHECALAIKRLRFEREGAAVQREIDRLQQAGRSEDIDRLWQRKHELLRR
                     LAALGMRGS"
     gene            799..1212
                     /locus_tag="PLT35_06620"
                     /pseudo
     CDS             799..1212
                     /locus_tag="PLT35_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            1819..2556
                     /gene="rpoD"
                     /locus_tag="PLT35_06625"
                     /pseudo
     CDS             1819..2556
                     /gene="rpoD"
                     /locus_tag="PLT35_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            2565..2640
                     /locus_tag="PLT35_06630"
     tRNA            2565..2640
                     /locus_tag="PLT35_06630"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2598..2600,aa:Ile,seq:cat)
     gene            2647..3417
                     /locus_tag="PLT35_06635"
     CDS             2647..3417
                     /locus_tag="PLT35_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOG28941.1"
                     /translation="MHPDLERVVRLQRIEDAAERARRTIADEPARQEALAAALDAARQ
                     ALETQRALLAENQTARRDAEKELAVQQARLSKYRDQLMEVKTNREYQALQKEIEVAQQ
                     EIRKQEDALLERMIEFDEVRERVRAAEEAFERDRAAFDAGREALAAEVAGAGEAIGTL
                     AAERAAVAAELSPPVLALFERVLRHRRTSAVAPLRDGRCAACQVRIRPQTVQEIRRND
                     SVVQCESCQRILYLEAPAAEGAAADAGASGAASARLQE"
     gene            3422..3880
                     /locus_tag="PLT35_06640"
     CDS             3422..3880
                     /locus_tag="PLT35_06640"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOG28942.1"
                     /translation="MIVAHIDGGSRGNPGPAGFGVRLSRPDGSLVAEFGEAIGIATNN
                     VAEYRGLLAALRYAVEHGHRNLLVRSDSLLLVCQMRGEYKVKNAGLQPLHAEARLLAA
                     RLDRVRFEHLPREQNARADRLANQAMDEAERAAVTPRPPGAVVLDPTPRA"
     gene            complement(3900..5345)
                     /locus_tag="PLT35_06645"
     CDS             complement(3900..5345)
                     /locus_tag="PLT35_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG28943.1"
                     /translation="MIPGAAGDDPANLELSVDDMRRLGEDALARVLAHVASIGAQPVR
                     GEVAAAELCAAMREAAPEHGAPIETLLDPLFRDWIPRSFTTPAPGYMAYIPGGGLYPA
                     AVADFISAATNRFTGLWQAAPALVQLEANVLDWFRQWMSFPPETRGLLTTGGSMAAFN
                     AVLCARERHLGRDVRPGVLYLSTQAHHSLEKAAKLAGVFHDRVRHVAVDAQYRMRVDA
                     LLDAVREDRAAGLAPFMVVSSAGTTNTGAVDPLDAIADACAAEGLWHHVDGAYGAFFH
                     LCPELRPILAGLPRADSLTLDPHKGLFLPYGTGALLVRDGAALRAVHGMSAGYLPATP
                     DLDAFYDPGQYGPDLSRGFPGLRVWLCLKVFGAARFRAALAEKRNLAEQACRRIAALP
                     GIVMTAPPHLSLFAFHVTRPGATRAEENLATAALLERVTRRGRVMLTGCTVDGGRFLG
                     RVCVLSFRTRQAHVDACVEDVAQELPFVWEA"
     gene            complement(5451..>5707)
                     /locus_tag="PLT35_06650"
     CDS             complement(5451..>5707)
                     /locus_tag="PLT35_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HOG28944.1"
                     /translation="LRAPLFLDTWAANADQLRLAGVPPAQVHALRLCTSCYREVFHSH
                     RVDGDRAGRMAGVIRKRPGPEGREPASGIGTSRFGGRRRG"
BASE COUNT          948 a         2012 c         2059 g          688 t
ORIGIN      
        1 ggtcgtgctg agcttcgatc ccgacgcggc ggggcagggc gcggcggcgc gctcgtgcga
       61 cctgctggtg gccgaggggt tccaggtgaa cgtggcggtg ctcccgggcg gggacgatcc
      121 cgacgcgttc atccgccgtc actcggggcg ccagtacgcc gcgcagctgc agcagtccac
      181 gccctacctc gactacctgc tggagcgcac ggcgtcggcc cacgacgtgc gccgcgacga
      241 cagccggcgc gaatttctcc aggccatgct cggcgtggcg gcgcggatcc cggacgcggc
      301 ggcccgcgat cagttcgcgg accgcatcgc ccaccgcgcg cggattgccg agtccgtcgt
      361 ccgggacgag atccgcaagg cggcggtgga gcggcggacc gagatcgggc cgcgcgagat
      421 gccggcgctg ggccagatca agccggcgga gcgcgggctc atctgggcgg cgatgcgcga
      481 gcccgacgag gcggccgacg cgctggccgc cctggacgag gaggacctgg agggcctggc
      541 ggccgcgtcc atcctcggac aggccctgcg ctggcaggct tcggagggca tccgctcacc
      601 cgcggccctg ctcgcgcgtc taaccaggga agaggcccag atcgtgaccg ggatcgcgtc
      661 cgcgccggcg tcgccggccc cggcccacga gtgcgccctg gccatcaagc gcctgcggtt
      721 cgagcgggaa ggcgcggccg tgcagcggga gatcgaccgc ctgcagcagg ccggacggag
      781 cgaagacatc gatcggttgt ggcagcggaa acacgaactg ctccggcgcc tggcggcgct
      841 ggggatgagg ggcagttgag ggaggcaccc ttgtcaatcg aggacaagtt cgacgaagtc
      901 cagcagctca tcatcatcgg caaggagaag ggctacctgc tgtacgacga ggtcaacgac
      961 ctcctgcgcg cggacatcac gtcggcggac gagctcgacg atctcttcag caccttcggc
     1021 agcgcgggaa tcgaggtcgt cgactccgac cagaagttcc gggaggagaa gcaggagggc
     1081 gacgagctgg agctcgacct ctcgcccggc accctcgaca agacgaacga tcccgtgcgc
     1141 atgtacctgc gcgagatggg aaccgttccg ctgctgaccc gggaaggcga ggtcgagatc
     1201 gccaagcgca tcgagcgcgg caagctggcg gtcatcaagt cgatcacgcg gacgccgacg
     1261 gtcgcggaga agaccatcgc catgggcgac gccctgaagg ccggccagcg ctccatccgc
     1321 gagctggtgg tgttcaacga cgaggagatc accgacgatc gcctggcgga gcggacccgt
     1381 gaggtgctca ggcagatcga ggcggtgcgc aaggcccggg cggcgtacgg caggctgcag
     1441 gagaagctgg acgccacggc gaagaaggac aagcggaagt accgccgggc gcgctggaag
     1501 gccatgcgcg cccgcatcgc cgtgtcccgg tgcatccggg agatcgagtt caccgagccg
     1561 gtcaagcggc gcctgatcga cgacatcaag gaggccgtcg agcgcatgag ccgcgtgcag
     1621 cgcgaggccg acaccctgag gcggcaactc gacgcgcggg cccgccggcc gaagctgaag
     1681 gaggacgagc gcagggcgct cgccagccgg atgaaggagc tgcgcggcga ggtgcggcgc
     1741 caggccgagc tgctcggcga gacgcccgac tacctccggc gcaccctcga gaccattcgc
     1801 cgcggcgagg cccaggccga acaggcgaag aaggagctgg tcgaggcgaa cctgcgcctc
     1861 gtcgtctcga tcgccaagaa gtacaccaac cgcgggctgc agttcctgga cctcatccag
     1921 gaaggcaaca tcggcctgat gaaggccgtg gacaagttcg agtaccgccg cggctacaag
     1981 ttctcgacct acgcgacgtg gtggatccgc caggcgatca cccgcgccat cgccgaccag
     2041 gcgcggacga tccgcatccc ggttcacatg atcgaaacga tcaacaagct catccggacg
     2101 tcgcgatcgc tcgtgcagga gctgggccgc gagcccacct ccgaggagat cgccgtccgc
     2161 atggacatcc cggtgtcgaa ggtccggaag gtgctcaaga tcgcccagga gccaatctcg
     2221 ctcgagaccc ccatcggcga ggaggaggac tcgcacctcg gcgacttcat cgaggaccgc
     2281 aacatcgtgt cgccgtccga cgcggtgatc aacctgaacc tgaaggagca gaccgactcg
     2341 gtgctgaaga ccctgacgcc gcgcgaggag aaggtcatca agatgcggtt cggcgtcggc
     2401 gacggctcgg agcacaccct cgaggaagtg ggccagagct tcgcggtcac ccgcgagcgc
     2461 atccgccaga tcgaggcgaa ggcgctcagg aagctccggc acccgtcgcg cagccggaag
     2521 ctgcgggcgt ttctcgaagg gcgctcttcg cagtaagctt atgcgggccc atagctcagc
     2581 ggtgagagcc gccggctcat aaccggcccg tcccaggttc gaatcctggt gggcccacca
     2641 cgcgacatgc accctgatct cgaacgcgtc gttcgtctcc agcggatcga ggacgccgcc
     2701 gagcgcgcgc gccgcacgat tgccgacgag cccgcccgcc aggaggccct ggcggcggcg
     2761 cttgacgccg cgcgacaggc cctcgagacg cagcgcgctc tgctggccga gaaccagacg
     2821 gcccgccgcg acgccgagaa ggagctcgcc gtgcagcagg cccggctgtc gaagtaccgg
     2881 gaccagctga tggaggtgaa gaccaaccgc gagtaccagg cgctgcagaa ggagatcgag
     2941 gtcgcccagc aggaaatccg gaaacaggag gacgccctcc tggagcgcat gatcgagttc
     3001 gacgaggtcc gagagcgcgt gagagccgcc gaagaggcgt tcgagcggga tcgagccgcc
     3061 ttcgacgccg ggcgggaggc cctcgcggcc gaggtggccg gcgccggcga ggccatcggg
     3121 acgctggccg ccgagcgcgc ggctgtcgcc gccgagctca gcccgccggt cctggcgctg
     3181 ttcgagcgcg tgctgcggca ccgcaggact tccgccgtgg cgcccctccg cgacggccgg
     3241 tgcgccgcct gtcaggtccg catccgcccg cagacggtcc aggagatccg ccggaacgac
     3301 agcgtggtcc agtgcgagag ctgtcagcgc atcttgtacc tcgaggcgcc cgccgccgag
     3361 ggcgcggcgg ccgacgccgg cgcctcgggc gcggcctcgg cccggctcca ggagtagcgg
     3421 cgtgattgtc gcgcacatcg acggcgggtc ccgcgggaac ccgggcccgg ctggattcgg
     3481 ggtgcgcctc tcgcggcccg acggctcgct cgtcgcggag ttcggcgagg ccatcggcat
     3541 tgccaccaac aacgtggcgg agtaccgcgg cctgctcgca gccctgcgct acgccgtgga
     3601 acacggccac cggaacctgc tggtccgatc cgactccctg ctgctcgtct gccagatgcg
     3661 cggcgagtac aaggtgaaga acgcgggcct ccagccgctg cacgccgaag ccaggctgct
     3721 cgccgcccgc ctcgaccggg tgcgcttcga gcacctcccc cgcgaacaaa acgcccgcgc
     3781 ggatcgcctc gccaaccagg cgatggacga agcggagcgg gcggctgtca caccccggcc
     3841 cccaggcgcc gtagtcctgg atccgacccc gcgggcctga cgggccgggc gaggcagcct
     3901 caggcctccc acacgaacgg gagttcctgc gccacgtcct cgacgcacgc gtccacgtgc
     3961 gcctggcgcg tgcggaagct cagcacgcag acgcgcccga ggaaccgccc gccgtccacc
     4021 gtgcagcccg tgagcatgac gcggccgcgg cgcgtaaccc gctcgaggag cgcggcggtc
     4081 gccaggttct cctccgctct cgtcgcgccg ggcctcgtca cgtggaacgc gaagagcgac
     4141 aggtgcggcg gggccgtcat gacaatgccg ggcagggcgg cgatgcggcg gcaggcctgt
     4201 tccgccaggt tccgcttctc cgccagcgcc gcgcggaagc gggccgcgcc gaagaccttg
     4261 aggcacagcc agacccgcag ccccgggaat ccccgcgaca agtccggtcc gtactgaccg
     4321 gggtcgtaga acgcgtcgag gtccggcgtc gccggcaagt accccgcgga catgccgtgc
     4381 acggcgcgca gcgcggcgcc gtcacgaacc agcagggcgc ccgtgccgta cggcaggaac
     4441 aggcccttgt gcggatccag cgtcagcgag tccgcgcgcg gcagcccggc aaggatcggc
     4501 cgcaactccg ggcagaggtg gaagaaggcg ccgtacgcgc cgtcgacgtg gtgccagagc
     4561 ccctcggcgg cgcacgcgtc cgcgatggcg tcgagcgggt ccaccgcccc ggtgttcgtg
     4621 gtgccggccg acgacaccac catgaacggc gccagtcccg cggcccgatc ctcgcgcacc
     4681 gcgtccagca gcgcgtccac gcgcatccgg tactgcgcgt ccaccgcgac gtgccgcacc
     4741 cggtcgtgga acacccccgc gagcttcgcg gctttctcca gcgagtggtg cgcctgcgtc
     4801 gacaggtaga gcacgcccgg ccgcacgtcg cggccgaggt gccgttcgcg cgcgcagagg
     4861 acggcgttga acgccgccat cgagccgccc gtcgtgagga gcccgcgcgt ctcgggcggg
     4921 aacgacatcc actggcggaa ccagtcgaga acgttggctt cgagttgcac cagtgccggc
     4981 gcggcctgcc agagccccgt gaagcggttc gtcgcagcgc tgatgaagtc cgccacggcc
     5041 gccgggtaga gcccgccgcc cgggatgtag gccatgtagc ccggcgccgg cgtggtgaag
     5101 gaccgcggga tccagtcgcg gaacagcggg tccagcaggg tctcgatcgg cgcgccgtgt
     5161 tccggcgcgg cctcgcgcat cgccgcgcac agttcagcgg cggcaacctc gccccggacg
     5221 ggctgggcgc caatggaggc cacgtgcgcg aggacgcggg cgagggcgtc ctcgccgagg
     5281 cggcgcatgt cgtcgactga aagctcgaga ttggcgggat cgtcccccgc agcgccagga
     5341 atcacgtcag gcgtactcct caacatgacg gccagactag cacgatgcga gcccgtccgc
     5401 gatcagcggc ccgtcgtcga agcgggcccg cgacggcggg gcgcaggaag tcagccgcga
     5461 cggcgcccgc cgaaccgcga ggtcccgatt ccggatgccg gctccctgcc ctcaggtccc
     5521 ggacgcttcc tgatcactcc cgccatgcgc cccgcccgat ccccgtcgac ccggtgcgag
     5581 tggaacacct cgcggtagca ggacgtgcac aggcgcaagg catggacctg tgccggcggc
     5641 acgcccgcca ggcgcaactg gtcggcgttg gcggcccacg tgtcgaggaa gagcggggcg
     5701 cggagac
//
LOCUS       DAOSIF010000157         5605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_12989_length_5605_cov_4.899820, whole genome shotgun sequence.
ACCESSION   DAOSIF010000157 DAOSIF010000000
VERSION     DAOSIF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12989_length_5605_cov_4.899820"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PLT35_06655"
     CDS             complement(<1..1243)
                     /locus_tag="PLT35_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOG28945.1"
                     /translation="MFKKLRELAVNLTVYGIGDVAVQLASFLLLPVYTIVLSPTEYGA
                     IALLLIVEQVVRVLNRWGIDAAFMRFYFDCADTAARRLLASTIFFFLAGAGGLLAAAG
                     LAAAPHLARPLFGGGDFTAAFRVVMITTLFGAVSFLPFHVLRIEGRARTFVALTFANN
                     LATLVAKLLLVVGLRKGVMGVVLADLLVAVGLALALLPRYAALIRPVFSLRVLAECLK
                     FGLPRVPHSFAHQIIAVADRVTLSRFAPLQQLGVYNVGASLGLGLKLFLSAFETAWAP
                     FYFKEMHAPNAKRTYRTMTTYAFGALALLVAGLAAVSRDLVRLMTVPRFHGAGDIVPW
                     IGLGVGLQGIYLLTSIGLNITKRTVYYPVATGLAAAAALGASLVLVPRYGAIGAAWSN
                     VAAYGVLAAAGMALSQRFYPIA"
     gene            complement(1288..2391)
                     /locus_tag="PLT35_06660"
     CDS             complement(1288..2391)
                     /locus_tag="PLT35_06660"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG28946.1"
                     /translation="MDFPRTRRGRPAVALVMALAALLWAPAARAADPQAPGAAQPSLL
                     DQMRSEAKSAIAAAVAAGRPGEALETYDRLFASVKKHDLALLAPVASGLLGAIASDVS
                     SLARIPALERLARSGDAAARGLLESAAGGRNTLMPAGIEADCALARLGDSPAVDRLIR
                     RLPEEVPRDKGAIITALKDAGAKRAAYAIVPFLGDANPSNRLAAIRALAEIGSREHVP
                     AMRAAFEAETHAGMRQSLAMALHAVGSNAGDAVLAQAEASRVPDVQLLALEAYHASGS
                     PRWPALARELLRSPGEGARLRAAQLLGVSDADAMRELARAASSPNTPTREVGARLLES
                     AGCRDAALLVTLLHDASPFVRTYAAGAALAAAK"
     gene            complement(2402..2593)
                     /locus_tag="PLT35_06665"
     CDS             complement(2402..2593)
                     /locus_tag="PLT35_06665"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28947.1"
                     /translation="MNCGTGGSACSVTMPASMTLGLQATPAAGYTFTGWTGNCAGSTA
                     NLFLALTGARTCSATFTAR"
     gene            2682..>3238
                     /locus_tag="PLT35_06670"
     CDS             2682..>3238
                     /locus_tag="PLT35_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28948.1"
                     /translation="MHVVAPSTVTVVVRPVPVQRPPQPVKVWVASGAAVSVTVVAAGK
                     VTEQAEPPAPQVSPPPVTVPPVGAGVIANPKATVGAKTAVQDLMPSTVTVVFGLVPLQ
                     APPQPVKVWVASGAAVSVTVVAAGKVTEQAEPPAPQVSPPPVTVPPVGTGVIASWWVM
                     LGVVPAGLKVALQVRAPVSARKRFAV"
     assembly_gap    3239..3338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3339..>4893)
                     /locus_tag="PLT35_06675"
     CDS             complement(<3339..>4893)
                     /locus_tag="PLT35_06675"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28949.1"
                     /translation="TFAASPGPIGSSINQFTGAVQYQDLAYSSCQQMYLAVWGSGGVI
                     KGRFVGESGAGAGDAFDISTGGWAQRPRVVYNPNLGRFLVAWHSGASESRTEVRAAAV
                     TYPPGPAAAGTVLSAPTYSTVSSARPAIAYSTGSGRYLVAWPRSSSSGTEINARLLDA
                     SGAPVGDEFAVTSTSSEAEREPAAAYFPAVNRFIVTWSGTGGASDFVRGRLYDAGTAA
                     PYGTTLSLAEGSFVHVPDAALNSVTGNVLVAWMQLDTATGGPRPFGRFVSGAGTLLSA
                     APSRLSAVAGAFLANALAFNPLSQSFLLVTHGSDTLQDVGFEIDGAGAPIGAAAAVTS
                     VTAPDLSGSFYPRIAASTRQPRWLIGTAASSTSLWTQLVGGRQPAAAVTYQLNVTKPT
                     GGSVSGGGIACGPAGTACSATFSSATTVTLTAAPEGGYTFTSWGGGCSGAGTTTTVLV
                     DGVKTCTASFAAASTYVMTIGPRPSGGTVTGAGLNCGAGGSACSVTMPAPMTLGLQAT
                     PAAGYTFTG"
     assembly_gap    4894..4903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4904..>5605)
                     /locus_tag="PLT35_06680"
     CDS             complement(<4904..>5605)
                     /locus_tag="PLT35_06680"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28950.1"
                     /translation="QQDSATGGWRPFGRFVSSAGAVLTSARRLSSTVGSYDANSVAYN
                     GLTGTFFLVTHLQGTPQDMGFEITGAGSPVAAATTVTSVTAENLTGSYNPRLAASSQR
                     ARWLIVTAASFASLWGQLIGEGGAPGGGDDGGGSGTGGTANPQLTISPAPTGGTVSGG
                     GLNCGTGGTTCQLTFTSPSSVTLSATPSPGYAFSGWGGACSGTGTTTTVTVNAALTCT
                     ATFTVPWSGSGTEPSG"
BASE COUNT          828 a         1935 c         2037 g          695 t
ORIGIN      
        1 aggcaatcgg atagaaccgc tgcgagagcg ccatgccggc cgccgccagc acgccgtatg
       61 ccgcgacgtt cgaccaggcc gccccgatgg ccccgtaccg cggtacgagc accaggctcg
      121 cgcccagggc ggccgcggcg gccagccccg tcgcgacggg gtagtacacc gtgcgcttcg
      181 tgatgttgag gccgatcgac gtcagcaggt agatcccctg caggccgacg cccaggccga
      241 tccagggaac gatgtcgccg gcgccgtgga agcggggcac ggtcatcagc cgcacgaggt
      301 cgcgggaaac ggcggccagg ccggccacca gcagggccag cgccccgaac gcatacgtcg
      361 tcatcgtgcg gtacgtccgc ttggcgttcg gcgcgtgcat ctccttgaag tagaagggcg
      421 cccacgcggt ctcgaacgcg ctgaggaaca gcttgaggcc gaggccgagg ctggcgccga
      481 cgttgtagac gcccagctgc tgcagcggcg cgaagcggct cagcgtgacg cgatcggcca
      541 ccgcgatgat ctggtgcgcg aagctgtgcg ggaccctcgg caggccgaac ttcaggcact
      601 ccgccagcac gcgaagcgaa aacacgggcc ggatgagcgc cgcgtagcgc ggcagcaacg
      661 ccagcgccag gccgaccgcc acgagcaaat ccgccagcac cactcccatc acgcccttgc
      721 gcagccccac gaccagcagc agcttcgcga cgagcgtcgc gaggttgttg gcgaaggtca
      781 gcgccacgaa ggtccgcgcg cgcccctcga tccggagcac gtggaacggc aggaaggaca
      841 cggcgccgaa cagcgtcgtg atcatgacga cgcggaaggc cgccgtgaaa tcgccgccgc
      901 cgaagagcgg ccgggcaagg tgcggcgccg ccgccaggcc cgccgccgcc agcaggccgc
      961 ccgcgcccgc caggaaaaag aagatcgtgc tcgccagcag gcgccgcgcg gccgtgtcgg
     1021 cgcagtcgaa gtagaagcgc atgaacgccg cgtcgatgcc ccagcggttc aggacccgca
     1081 cgacctgctc cacgatcagc aggagggcga tcgcgccgta ctcggtgggc gagaggacga
     1141 tcgtgtagac gggcagcagg agaaagctgg cgagctggac ggcgacgtcg ccgatgccgt
     1201 acaccgtcag gttgacggcg agttcgcgca gcttcttgaa cacggccccc gattgtagcc
     1261 cagcggcgcg cgcgccgccg ggccccgtca cttcgcggcg gcaagcgccg cgccggccgc
     1321 gtaggtgcgg acaaacgggc tcgcgtcgtg gaggagcgtc acaagaagcg ccgcgtcacg
     1381 gcagccggcc gactcgagga gtcgcgctcc gacttcgcgg gtgggcgtgt tggggctgga
     1441 agccgcgcgc gcaagctcgc gcatcgcgtc ggcgtccgac acgccgagca actgggccgc
     1501 gcgcaggcgc gccccctcgc ccggcgaccg cagcagctcc cgcgcgagcg ccggccagcg
     1561 cggcgacccg gaggcatggt aggcctcgag cgcaagcaac tggacgtccg ggaccctgct
     1621 cgcctcggcc tgcgccagca ccgcgtcgcc ggcgttgctg ccgacggcgt gcagggccat
     1681 cgccaggctc tgccgcatgc ctgcgtgcgt ctccgcctcg aaggccgcgc gcatggccgg
     1741 aacgtgctcg cgggaaccaa tctcggcaag cgcccggatc gccgcgagac ggttcgacgg
     1801 gttcgcgtct cccaggaacg gcacgatggc gtacgccgcc cgtttcgcgc cggcgtcctt
     1861 cagcgcggtg atgatggcgc ccttgtcgcg gggcacctcc tcaggcagcc gccggatcag
     1921 gcggtccacc gcaggagaat cccccagccg agccagcgcg cagtcggcct cgatgcccgc
     1981 aggcatcagg gtgttgcggc ccccggcggc ggactcgagc agaccgcggg ctgccgcgtc
     2041 tcccgatcgg gcgaggcgct ccagcgcggg aatccgggcc agcgaggaca catcgctggc
     2101 gatggcgccg aggaggccgg acgccaccgg cgcgagcagc gcgaggtcgt gcttcttgac
     2161 ggacgcaaag agacgatcgt aggtctcgag cgcctcgccc ggccgcccgg ctgccacagc
     2221 ggcagcgatg gccgacttcg cctcggagcg catctgatcg agaagcgacg gctgcgccgc
     2281 gccgggagcc tgcgggtcgg ccgctcgggc ggctggcgcc cagagaaggg ctgcaagggc
     2341 catgacgagc gcgacggctg gccggccgcg tctggtgcgt gggaagtcca tctccgacag
     2401 tctacctcgc cgtgaaggtg gcgctgcagg tgcgggcgcc ggtgagcgcg aggaagaggt
     2461 tcgcggtgga gccggcgcag ttgcccgtcc agccggtgaa ggtgtagccg gcggcgggcg
     2521 tggcctgcag gccgagtgtc atcgaggccg gcatcgtgac cgagcaagcg gacccgcctg
     2581 tgccgcagtt cagcccggcg ccggtgatgg tgccgcccgt cgggacgggg ctgatcgtca
     2641 tcgtgtaggg cgggccggcg gccaggccgc cgaaggtggc ggtgcacgtg gtggcgccgt
     2701 ccacggtgac cgtggtggtg aggcccgtgc ccgtgcagcg gccgccccag ccggtgaagg
     2761 tgtgggtcgc gtcgggcgcg gccgtgagcg tgacggtggt ggcggcgggg aaggtcaccg
     2821 agcaggcgga gccgcccgcg ccgcaggtga gcccgccgcc ggtcaccgtg ccgcccgtcg
     2881 gcgccggcgt gatcgccaat ccaaaggcga cagtcggggc gaagaccgcc gtgcaggact
     2941 tgatgccgtc caccgtgacg gtcgtgttcg gactggtgcc gctgcaggca ccgccccagc
     3001 cggtgaaggt gtgggtcgcg tcgggcgcgg ccgtgagcgt gacggtggtg gcggcgggga
     3061 aggtcaccga gcaggcggag ccgcccgcgc cgcaggtgag cccgccgccg gtcaccgtgc
     3121 cgcccgtcgg caccggcgtg atcgccagct ggtgggtgat gctgggagtc gtgcctgcgg
     3181 ggctgaaggt ggcgctgcag gtgcgggcgc cggtgagcgc gaggaagagg ttcgcggtnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gccggtgaag gtgtagccgg
     3361 cggcgggcgt ggcctgcagg ccgagtgtca tcggggccgg catcgtgacc gagcaggcag
     3421 acccgcccgc gccgcagttc agcccggcgc ccgtgactgt tcccccggac ggcctgggac
     3481 cgatcgtcat gacgtacgtg gatgccgcgg caaatgacgc ggtgcaggtc ttcacgccgt
     3541 cgaccagcac cgtcgtggtc gtaccggcgc ccgagcagcc tccgccccac gacgtgaagg
     3601 tgtagccgcc ttccggcgcg gccgtgagcg tgaccgtcgt cgccgacgag aaggtcgccg
     3661 agcacgccgt tccggccggg ccgcacgcga tcccgccgcc gctgaccgac ccgcccgtcg
     3721 gcttcgtgac gttcagttga tacgtgacgg ctgccgccgg ctgtcttccg ccgacaagct
     3781 gcgtccacaa gctggtggac gaggccgcgg tgccgatcag ccaccgcggc tggcgcgtgc
     3841 tcgcggcgat cctcgggtag aacgaccccg agagatccgg cgccgtgacg gaagtgaccg
     3901 ccgccgccgc gccgattggc gcgccggcgc cgtcgatttc gaagccgacg tcctgcagcg
     3961 tgtccgagcc gtgggtgacg aggaggaacg actgcgacag cgggttgaag gccagcgcat
     4021 tcgccaggaa cgcacccgcg accgccgaca gcctcgacgg agcggccgac aggagcgtgc
     4081 ctgcgccgga gacgaatcgg ccgaacggcc tcggcccgcc ggttgccgtg tccagttgca
     4141 tccaggccac caggacgttg cccgtcacgc tgttcagggc cgcgtccggc acgtgcacga
     4201 agctgccctc cgcgagcgac aacgtcgttc cgtacggcgc ggccgtcccc gcgtcgtaca
     4261 gccggccgcg cacgaagtcc gaggcccctc ccgttccgga ccacgtcacg atgaaacggt
     4321 tgaccgcagg gaagtaggcc gccgccggtt cacgctcagc ctcgctggag gtcgaggtga
     4381 ccgcgaactc gtctccaacg ggcgcgccgg aagcgtcgag cagcctggcg ttgatctccg
     4441 tgcccgagga cgacgaacgc ggccacgcca ccaggtagcg gccggaaccc gtcgagtacg
     4501 cgatcgccgg tcgggcagac gagacggttg agtacgtcgg tgcggacagc acggtccctg
     4561 cagccgcagg cccgggtggg taggtcaccg ccgccgcccg gacttccgtg cgggactcgc
     4621 ttgcgccgct gtgccacgcg accaggaagc gcccgagatt cgggttgtag acgaccctcg
     4681 ggcgctgggc ccagccgccc gtcgagatgt cgaacgcgtc gccggcaccg gcgcccgact
     4741 cgccgacgaa gcgtcccttg atgacgccgc ccgatcccca caccgcgagg tacatctgct
     4801 ggcacgagct gtacgccagg tcctggtact gcacggcgcc ggtgaactgg ttgatcgacg
     4861 atccaatcgg tccgggcgag gcggcaaagg tgannnnnnn nnnccggacg gttcggtgcc
     4921 cgatccggac cagggcaccg tgaaggtggc ggtgcacgtc agcgccgcgt tcaccgtcac
     4981 ggttgtcgtc gtgcccgtgc ccgagcaggc tccgccccag ccggagaagg cgtagcccgg
     5041 gctcggcgtg gccgagagcg tcaccgagct cggcgacgtg aaggtgagct ggcaggtcgt
     5101 tccccccgtg ccgcaattca gtccgccgcc gctgaccgtc ccgccggtgg gcgccgggga
     5161 gatcgtcaac tgcgggttcg ccgtgccgcc cgtgccgctt ccgcctccgt catcgccgcc
     5221 gccgggcgcg ccgccttctc cgatgagctg gccccacagg ctcgcgaacg aggcagccgt
     5281 gacgatcagc catcgtgccc gctgcgaact ggccgccagc ctggggttgt acgatccggt
     5341 gaggttctcc gcggtcaccg acgtgaccgt cgtcgcggcc gcgaccggcg atcccgcgcc
     5401 ggtaatctcg aagcccatgt cctggggcgt gccctggaga tgcgtcacga ggaagaacgt
     5461 gccggtcagg ccgttgtagg cgaccgagtt cgcatcgtag gacccgaccg tcgaggacag
     5521 gcgccgcgcc gacgtcagca ccgcgcccgc gctgctgacg aaccggccga agggccgcca
     5581 tccgcccgtg gccgaatcct gctgg
//
LOCUS       DAOSIF010000158         5598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13005_length_5598_cov_2.987913, whole genome shotgun sequence.
ACCESSION   DAOSIF010000158 DAOSIF010000000
VERSION     DAOSIF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13005_length_5598_cov_2.987913"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLT35_06685"
     CDS             <1..800
                     /locus_tag="PLT35_06685"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HOG28951.1"
                     /translation="ARIPIVAAFIYNLKYRDDKQNAIDPSLDMGANFAHMIGQSLEYR
                     DVARMYFILHSDHESGNVSAHATHLVHSALSDPYYAYSAGLNGLAGPLHGLANQEVLS
                     WTMAFQKKYCADREPTKELVTKALWDTLNAGQVIPGYGHAVLRKTDPRYMSQREYCLN
                     TPGLRDDPLFKLVSMIFEVAPGVLTEHGKTKNPWPNVDAQSGVIQWYYGVKEWDYYTV
                     LFGVGRALGCMANITWDRGLGYAIERPKSVTTAMLEKWAAEGGRKLG"
     gene            complement(806..2575)
                     /locus_tag="PLT35_06690"
     CDS             complement(806..2575)
                     /locus_tag="PLT35_06690"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOG28952.1"
                     /translation="MPRSVRIRQAARAAACLFVSAAAAAVAAPVRLVDQAPVRIDAVA
                     PGVALADFGRAAFGNLCIVPPAGARGEITVHFGEALAGGRVNRQPPGSVRYAPVRVVL
                     DGRASIIVAPPPDARNTRPPSAILTPPGWGVVLPFRWVEIEGWPGEIRAGHIVRRAAF
                     AAAWDDGAASFRSSDGLLDRIWELCRYSIKATTFAGVYVDGDRERIPYEADAYISQIS
                     HYATDRDVQMARDTFDRLMRHPTWPTEWAFHMVFMAHAEWMHTGDLAWLRERYEALKP
                     KLLLDRARADGLLASSEAQRTREDIVDWPAGERDGFVFTPVNTVVNAFHLRSLALMAE
                     LADAVGRTSEAEGYRQRARTARTAFQQLLFDRSRLVYRDGEGTDHASLHASLFPLAFG
                     LVPSEHVPPVIARLSAKGMACSVYAAQYLLEGLFEHDAAAQALALMTAQHDRSWRHMV
                     DSGATITWEAWDQKYKPNLDWNHAWGAAPANLLPRFVLGAQPLAPGWLRARVRPRAGS
                     LSHAAGTIPTPRGPIRIGWSRETGFRMRLDLPPGMSARVDLPAEPGSLGVYLRSTPVR
                     AERVGAYWRLAEDVRGSVVLDVR"
     gene            complement(2613..4877)
                     /locus_tag="PLT35_06695"
     CDS             complement(2613..4877)
                     /locus_tag="PLT35_06695"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOG28953.1"
                     /translation="MIKISDEDVFQYHEKPRPGKLEVSTSKPCLTQRDLSMAYTPGVA
                     RPCLAIEQDPELAYRYTGKGNLVAVVSNGTAVLGLGDIGAVAGKPVMEGKGVLFKRFA
                     GVDVFDIELNTHDPDEIIRIVKALEPTFGGINLEDIKAPECFYIEESLKKIMDIPVFH
                     DDQHGTAIISGAALVNALELVNKKIGDVKVVFAGAGAAGIACAEMYLRLGVSKANILF
                     VDTVGVIWNGRAEKMNPYKQRFAADTKARTLADALKDADVFVGVSARDLVTPEMLKTM
                     ATDPIVFAMANPDPEIPYPVATATRQDVVMATGRSDYPNQVNNVLGFPFIFRGALDVR
                     ATAINDEMKVAAAHALAALAKEDVPETVLKAYGVNSMAFGREYLIPKPFDPRVLTWEA
                     PAVAKAACETGVAKRPISDWDAYREQLERMLGPSRKVMHFVVRKARNDAPRRIVFPEG
                     EQDAVIRAARDIVDQKIAMPVLLGKEADIRARASSLGIDQLEFEIVDVPNAERGPVYA
                     EALYDLRKRKGVTLPAAHDLVKDPTIFALMMVQRGDADGFVGGMYKAYPETIRPALQI
                     VGLKPGVSRVSAAHVLVLKDRLFFCADTMVNIEPTSEELAEIALLAADLARSFDTDPK
                     VALLAFSSFGSVPHPISKRVARAVDLVREQAPSIVVDGEMHLDTAVVEEIVEKNYPHS
                     RIKGDANVLVFPDLTSGNIGYKLVQRLGNADAIGPLLMGMRKPVNVLQHGMTVAEIVN
                     VAAITAVTAEALRR"
     gene            5195..>5598
                     /locus_tag="PLT35_06700"
     CDS             5195..>5598
                     /locus_tag="PLT35_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28954.1"
                     /translation="MKTFSMKIDPLTKEEYWEVYLRGQQILSDPLLNKASAFTQEERL
                     SLGLTGLLRTAVSTLDRQVERSYENLQRKPDDLERYIYLQGLLDRNEVLYYRVLQEHL
                     TELVPIVYTPTVGQACLELSHITRRYRGVYLSP"
BASE COUNT          852 a         1952 c         1931 g          863 t
ORIGIN      
        1 tggcccgcat cccgatcgtc gcggccttca tctacaacct gaagtaccgg gacgacaagc
       61 agaacgccat cgatccgtcg ctggacatgg gcgcgaactt cgcccacatg atcggccaga
      121 gcctggagta ccgcgacgtg gcccggatgt acttcatcct gcactccgac cacgagtcgg
      181 gcaacgtctc ggcgcacgcg acgcacctgg tgcactcggc gctctccgat ccctactacg
      241 cgtactcggc cggcctgaac ggcctggcgg gcccgctcca cgggctggcc aaccaggaag
      301 tgctgagctg gaccatggcg ttccagaaga agtactgcgc cgaccgggaa ccgaccaagg
      361 aactggtgac gaaggccctc tgggacacgc tgaacgccgg ccaggtcatt ccgggctacg
      421 gccacgcggt tctgcgcaag acggacccgc gctacatgtc gcagcgtgag tactgcctga
      481 acaccccggg cctccgggac gacccgctct tcaagctcgt gtcgatgatc ttcgaggtcg
      541 cgccgggcgt gctcaccgag cacggcaaga cgaagaaccc ctggccgaac gtcgacgcgc
      601 agtccggcgt catccagtgg tactacggcg tcaaggagtg ggactactac acggtgctct
      661 tcggcgtcgg ccgggcgctc ggctgcatgg ccaacatcac gtgggaccgc ggcctcgggt
      721 acgccatcga gcgtccgaag tcggtgacga cggcgatgct cgagaagtgg gccgcggagg
      781 gcggccgcaa gctcgggtaa tcccgctacc tgacatcgag gacgacggat cctctcacgt
      841 cctcagcaag ccgccagtac gcgccgactc gctcggcgcg tactggcgta ctgcggaggt
      901 acacgcccag cgacccgggt tccgcgggca ggtccacgcg ggccgacatg cccggcggca
      961 ggtcgagcct catccggaat ccagtctcgc gagaccaccc gatccggatc ggcccccgcg
     1021 gcgtgggaat cgtccccgcc gcatgcgaga gcgagccggc gcgcggccgg acccgggccc
     1081 ggagccagcc cggcgcgagg ggctgcgccc cgagcacgaa gcgcgggagc aggttggccg
     1141 gggccgctcc ccacgcgtgg ttccagtcca ggttgggctt gtacttctgg tcccacgctt
     1201 cccaggtgat ggtggcgccg ctgtcgacca tgtggcgcca gctgcggtcg tgctgcgcgg
     1261 tcatcagcgc cagcgcctgc gcggcggcat cgtgctcgaa gaggccctcg aggaggtact
     1321 gggcggcgta caccgagcag gccatgccct tcgccgacag gcgggcgatc acgggcggca
     1381 cgtgttcgct cggcacgagg ccgaacgcga gggggaacag gctggcgtgc agcgaggcgt
     1441 ggtccgtgcc ttcgccgtcg cggtagacga ggcgggaccg gtcgaagagg agctgctgga
     1501 acgccgtccg cgccgtccgg gcgcgttggc ggtagccctc ggcctcggac gtccgcccca
     1561 cggcatccgc cagctcggcc atgagcgcca gggaccggag gtggaacgcg ttgaccacgg
     1621 tgttcaccgg ggtgaagacg aagccgtcgc gctcccctgc cggccagtcg acgatgtcct
     1681 cgcgcgtgcg ctgcgcctcg ctgctggcaa ggaggccgtc ggcccgcgcg cggtcgagga
     1741 gcagcttcgg tttcagcgcc tcgtaccgct cgcggagcca cgcgaggtcg ccggtgtgca
     1801 tccactccgc gtgggccatg aagaccatgt ggaacgccca ctcggtcggc cacgtgggat
     1861 gcctcatcag gcggtcgaag gtgtcgcgcg ccatctgcac gtcgcgatcg gtggcgtagt
     1921 ggctgatctg gctgatgtag gcgtccgctt cgtacgggat gcgctcgcgg tccccgtcga
     1981 cgtagacgcc ggcgaacgtc gtggccttga tggagtagcg gcagagttcc cagatgcgat
     2041 cgagcaggcc gtcagatgag cggaacgacg cggccccatc gtcccacgcg gcggcgaagg
     2101 ccgcccggcg cacgatgtgc ccggcgcgga tctcgccggg ccacccttcg atctcgaccc
     2161 agcggaacgg caggacgacg ccccacccgg gcggggtgag gatggcggag ggcggcctgg
     2221 tgttgcgggc gtcgggcgga ggggcgacga tgatggaggc gcggccgtcg agcaccacgc
     2281 ggaccggcgc gtaccgcacc gagccgggcg gctgccggtt cacgcgtccc ccggccagcg
     2341 cctcgccgaa gtggacggtg atctcgccgc gggcccccgc gggcgggacg atgcacaggt
     2401 tgccgaaggc cgcgcggccg aagtcggcca aggccacgcc gggcgcgacg gcgtcgattc
     2461 ggacgggcgc ctggtcgacc agccgtacgg gggccgcgac cgccgccgcg gccgcggaca
     2521 cgaagaggca ggcggccgcg cgggccgcct gccggatccg gacggatcga ggcatgcgaa
     2581 cgtccgtccc ttcgccgcgc ggcggcgcca ggctacctcc tgagcgcctc cgccgtcacg
     2641 gccgtgatcg cggcgacgtt cacgatctcg gccacggtca tgccgtgctg cagcacgttc
     2701 accggcttcc tcatgcccat caggagcggc ccgatcgcgt cggcgttgcc caggcgctgg
     2761 accagcttgt agccgatgtt gcccgaggtg aggtccggga agacgagcac gttcgcgtcg
     2821 cccttgatgc gcgagtgcgg gtagttcttc tcgacgatct cctccacgac ggccgtgtcc
     2881 aggtgcatct cgccgtcgac gacgatcgag ggggcctgct cacgcacgag atcgacggcg
     2941 cgggccaccc gcttcgagat cgggtgcggc accgacccga agctcgagaa cgcgagcagg
     3001 gccaccttcg ggtcggtgtc gaagctgcgc gccaggtcgg cggcgagcaa ggcgatctcg
     3061 gccagctcct cggaggtggg ctcgatgttc accatcgtgt cggcgcagaa gaagagccgg
     3121 tccttcagca cgagcacgtg ggccgccgag acgcgcgaga cgcccggctt gaggccgacg
     3181 atctggagcg ccgggcggat ggtctccgga taggccttgt acatcccgcc cacgaagccg
     3241 tcggcgtcgc cgcgctgcac catcatcagc gcgaagatcg tcggatcctt cacgagatcg
     3301 tgcgccgcag gcagcgtgac ccccttgcgc ttccgcagat cgtagagcgc ctcggcgtag
     3361 acggggccgc gctcggcgtt cgggacgtcg acgatctcga actccagctg gtcgatcccg
     3421 aggctcgatg ccctcgcgcg gatgtccgcc tcctttccca gcaggacggg catggcgatc
     3481 ttctggtcga cgatgtcgcg cgcggcccgg atgacggcgt cctgctcgcc ctcggggaac
     3541 acgatccgcc tcggcgcgtc gttgcgcgcc ttgcgcacga cgaagtgcat gaccttgcgc
     3601 gacgggccca gcatgcgctc gagctgctcc cggtaggcgt cccagtcgga gatgggccgc
     3661 ttcgccacgc ccgtctcgca cgccgccttg gccaccgccg gcgcctccca ggtcagcacc
     3721 ctcgggtcga acggcttcgg aatcaggtac tcccggccga acgccatgct gttgacgccg
     3781 taggccttca gcacggtctc cgggacgtcc tccttcgcca gggccgccag cgcgtgcgca
     3841 gccgccacct tcatctcatc gttgatggcc gtcgcgcgca cgtccagcgc cccgcggaag
     3901 atgaacggga acccgaggac gttgttcacc tggttcgggt agtcggaccg gcccgtcgcc
     3961 atgacgacgt cctggcgcgt cgccgtggcc accggatacg ggatctccgg gtcgggattg
     4021 gccatcgcga acacgatcgg gtccgtcgcc atcgtcttca gcatctcggg ggtgacgagg
     4081 tccctggccg acacgccgac gaacacgtcc gcgtccttca gcgcgtcggc cagcgtccgg
     4141 gccttcgtgt ccgcggcgaa ccgctgcttg tacggattca tcttctcggc gcgcccgttc
     4201 cagatgacgc cgaccgtgtc cacgaacagg atgttcgctt tcgacacgcc gaggcgcagg
     4261 tacatctccg cgcaggcgat gcccgcggcg ccggctccgg cgaagacgac cttcacgtcg
     4321 ccgatcttct tgttcacgag ctcgagcgcg ttcaccaggg ccgcgcccga gatgatcgcc
     4381 gtcccgtgct ggtcgtcgtg gaacaccggg atgtccatga tcttcttcag ggactcctcg
     4441 atgtagaagc actcgggcgc cttgatgtcc tcgaggttga tgccgccgaa ggtgggctcg
     4501 agggccttca cgatcctgat gatctcgtcc gggtcgtgcg tgttcagctc gatgtcgaag
     4561 acatccacgc cggcgaaccg cttgaacagt acgcccttcc cttccatcac gggcttgccg
     4621 gcgacggctc cgatgtcgcc gagaccgagc accgccgtcc cgttcgacac gaccgccacg
     4681 aggttgccct tccccgtgta gcggtacgcc agctccggat cctgctcgat cgccaggcac
     4741 ggccgggcga cgcccggggt gtaggccatg gagagatcgc gctgggtcag gcagggtttc
     4801 gatgtgctga cctcgagctt cccgggcctt ggcttctcgt ggtactggaa gacgtcttcg
     4861 tcgctgatct tgatcatgat cctggcctct ccggttgtgg aaggacgcgg gcgaccaaca
     4921 cgcaccgacg acgggaacgc ggccgccggg cggccggcac gccgtatagc ctacccgatc
     4981 tcccgccggc ggaagccgcc attcgacgcg cccggcgaac cgacggcggc ccggggcctg
     5041 gaggccccgg gctccgtcag cgggaccgat ccgtgtccgc cgcagaggcc gggccgggac
     5101 gcctcgtgcg ttcatcctgg ggcgcgcgac gcgcgcgccc cgttcttgac accccccgtg
     5161 cgagtggtca gcatgggtgg caacggagtg tgccatgaag acgttctcga tgaagatcga
     5221 cccgttgacg aaggaggagt actgggaggt ttacctccga ggccagcaga tcctcagcga
     5281 tcctctgctc aacaaagcgt ccgcgttcac ccaggaggaa cggctctcgc tcggactgac
     5341 cgggctcctg cggaccgcgg tctcgaccct cgaccgccag gtggagcggt cctatgagaa
     5401 ccttcagcgc aagccggacg acctcgagcg ctacatctac ctccagggcc tgctggaccg
     5461 gaacgaggtg ctctactacc gggtgctgca ggagcacctg accgagctcg tcccgatcgt
     5521 ctacacgccg acggtcggcc aggcctgcct ggagctgagc catatcacgc gccggtaccg
     5581 cggcgtgtac ctgtcgcc
//
LOCUS       DAOSIF010000159         5583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13054_length_5583_cov_3.609986, whole genome shotgun sequence.
ACCESSION   DAOSIF010000159 DAOSIF010000000
VERSION     DAOSIF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13054_length_5583_cov_3.609986"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLT35_06705"
     CDS             <1..983
                     /locus_tag="PLT35_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28955.1"
                     /translation="QGGAMDYPLQLPEAKAVSDFLLAHPNVAGLQTYHNSGGMILRSP
                     GAEQIGEYPPQDTMVYDEIGRMGERILPFYRYLVIWSGLYTVHGGVTDWGNDTLGIIS
                     LSNELWSSQQYFTSPELKEQQRDPESPIAPRVANYFFNDYLEFGDQLTEWRTFNHPQF
                     GEVEIGGAFKKTFGRVPPRFMNEELCHRNMAFTLYQADEMPRMSIGATKVERLGGGVF
                     KVWVDLVNDKLAPTILAKAAQNAVVPPDLLTLDGKGVEVLSAGWVPSKWRPSVARPID
                     QKDLKRIIVRNGHPGRTTRTVQYLVKGSGPVTVTYASLKGGSPQKTILLQ"
     gene            complement(943..2685)
                     /locus_tag="PLT35_06710"
     CDS             complement(943..2685)
                     /locus_tag="PLT35_06710"
                     /inference="COORDINATES: protein motif:HMM:NF022115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOG28956.1"
                     /translation="MAGVSGRVWQRAAIYGSLWAASEIVAGSFLHNLRIPFAGSLLAA
                     SGVMMMTAGHRLHPERGLIWRAALVCALMKSISPSAVILGPMVGIAMEGLLLEAGVRL
                     ARGRAAGYLIGGAAAVSWSMAQRIITAIIAFGGDVVRLYVDAYRFAARSLAVESFGPF
                     DLIWTFVVLEWLAGLAAAAVGLRVAGRVGNKAAPLVPGAAAAPAVFPEPPIEAEGPWS
                     LRRLAAAAFVLVGGMMGLSRLPLAAAALVVAVFAAWVLAVYPRAAGRIRRLSLWLELL
                     AVMLLAGLVFGLVRHGASGLAGGLAEGAAMALRAVMVIVGFTAISAELRNPSILAFVE
                     RRKLRGLSDALGVAFGILPAFTGALADRRRSWRRPGRVLADLLAVADSLVLAHAPASS
                     CRRVVLLTGPTGSGKTTMATEVVKRLRQAGVRVGGVLAPGLLADERRTGFDLLDIATG
                     QRAGLARERPGPAARHAQWGRFSFLPDGLALGARALGPGAAASDVIVVDEVGPFELSG
                     GGWAEALDALARGFPGALLLVARESVAEAVAARWGAAETIVCRVDGDAPERVAGLLDA
                     RATGAGSSSAATRP"
     gene            complement(2693..3310)
                     /locus_tag="PLT35_06715"
     CDS             complement(2693..3310)
                     /locus_tag="PLT35_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOG28957.1"
                     /translation="MPLPSRYLVPAATIRAEREILRSRFITTVGRASTAEEAAAFVRA
                     VSGEHPGAAHNCWAYVVGPPGDTSRIGMSDDGEPHGTAGRPMLNALLRAGVGDIVAVV
                     TRYYGGTKLGTGGLVRAYGGGVQQALAALAVAERIDYLSLTAVVDYASLSALQQACAA
                     CEGEILRQEFAADVRVRLRLPAGRAGEFRSALLNATRGRARVAED"
     gene            3335..4270
                     /locus_tag="PLT35_06720"
     CDS             3335..4270
                     /locus_tag="PLT35_06720"
                     /inference="COORDINATES: protein motif:HMM:NF022665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HOG28958.1"
                     /translation="MKAYPIPAVLGLNSRWSTLITMEPLDDFRMQPEQEPPVPDAGGP
                     SFLPYWIAAAALVLAVAIGGAWWYRQRPSAAANQAPSPDGAGQAAETGGAHAPLGPAV
                     DPIELPPLVLTDPLVRDLLGQLSSSPTLASWLATDGLIRKAVASIENVAEGASPATHA
                     RVLAPRLPFAAETEGGTITIDPRSYARYDGIAGAVASLDPAGVARVYSLLKPRLAEAY
                     ADLGHPDADIDAAVERAVSMLLGAPVVEEPVVLRRRVLSYRYDRADFEALPAAQKQLL
                     RMGPRNTRIIQEQLRAVARELGIPAGRLPPRQTAR"
     gene            4376..4900
                     /gene="msrB"
                     /locus_tag="PLT35_06725"
     CDS             4376..4900
                     /gene="msrB"
                     /locus_tag="PLT35_06725"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOG28959.1"
                     /translation="MTRLQLAAAALCALVAAVVPWPHSWSTGTMSHDPSAPSKPFPLT
                     KTDEEWRAALTPMQFAVLRRGDTEYPGTSPLLHEHKPGVFRCAACGQALFHSAAKYES
                     GSGWPSFYDAVEDAVGTRQDRALGMLRTEVHCSRCGGHLGHRFPDGPEPTGQRYCING
                     AALAFEPGAPSPET"
     gene            complement(4976..>5583)
                     /locus_tag="PLT35_06730"
     CDS             complement(4976..>5583)
                     /locus_tag="PLT35_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28960.1"
                     /translation="EIYALDGRQRRVVRLDAAGTTAGVLAFDGVPPPATVIPKAMAID
                     ASDALYVLDVFSARVLVIDGEGRFRRALPVPEGAGFGTDIAIDQAGALVLLDSVGRRL
                     YAAAKGAAAFSSLGGDLSGWLASSPAHVAVSKGLIFVLEGSGGSIVGFGRDGSFLSRP
                     VVMGREEGALNRPSQICVNDSDEVFVADRDNSRIQVFQLLR"
BASE COUNT          883 a         2048 c         1955 g          697 t
ORIGIN      
        1 tccagggcgg ggcaatggac tacccgctcc agcttcccga ggcgaaagcc gtcagcgact
       61 tcctcctggc gcatccgaac gtcgccgggc tgcagaccta ccacaacagc ggcggcatga
      121 tcctgcgcag cccgggcgcc gagcagattg gcgagtatcc gccgcaggac acgatggtgt
      181 acgacgaaat cggccggatg ggcgagcgca tcctgccgtt ctatcgctac ctcgtgattt
      241 ggagcgggct ctacacggtg cacggcggcg tgacggactg gggcaacgac acgctgggca
      301 tcatctccct gtccaacgag ctgtggagct cgcagcagta cttcaccagc cccgagctga
      361 aggagcagca gcgggacccg gagagcccga tcgcgccgcg cgtcgcgaac tatttcttca
      421 acgactacct cgagttcggc gaccagctga cggagtggcg gacgttcaac cacccgcagt
      481 tcggggaggt cgagatcggc ggcgcgttca agaagacgtt cgggcgcgtg ccgccgcggt
      541 tcatgaacga ggagctgtgc caccgcaaca tggcgttcac gctgtaccag gccgatgaga
      601 tgccgcgcat gtcgatcggc gcgacgaagg tcgagcggct cggcggaggg gtcttcaagg
      661 tgtgggtgga cctggtcaac gacaagctcg cgccgacgat cctcgcgaag gccgcccaga
      721 acgccgtcgt gccgccggac ctgctgacgc tcgacggcaa gggcgtcgag gtgctgtcgg
      781 ccggctgggt gccgagcaag tggcgtcctt cggtggccag gccgatcgat cagaaggatc
      841 tgaagcgcat catcgtgcgc aacggccatc ccggccggac gacgcgcacg gtgcagtacc
      901 tcgtcaaagg ctcgggccct gtcacggtga cgtacgcgag cctcaagggc gggtcgccgc
      961 agaagacgat cctgctccag tagcgcgggc gtcgaggagc ccggccacgc gctccggggc
     1021 gtcgccgtcc acgcggcaga cgatcgtctc ggcggcgccc caccgcgcgg ccaccgcctc
     1081 ggcaaccgac tcgcgcgcga cgagcaggag cgcgccgggg aatccccgcg cgagcgcgtc
     1141 cagggcctcg gcccatcctc ctccggacag ctcgaagggc ccgacctcgt cgaccacgat
     1201 gacatccgac gccgcggcgc ccgggccaag ggcgcgcgcg ccgagcgcca gtccgtccgg
     1261 caggaacgaa aaccggcccc actgcgcgtg ccgggccgcc gggcccgggc gctcgcgggc
     1321 gaggcccgcc cgctggcccg tcgcgatatc gagcagatcg aaaccggttc gccgctcgtc
     1381 cgccagcagg cccggcgcaa ggacgccgcc gacgcggacg cccgcctggc gcagccgctt
     1441 cacaacctcc gtcgccatcg tcgtcttgcc cgacccggtg gggcccgtga gcaggacgac
     1501 ccggcggcag gacgatgcgg gcgcgtgcgc gagcacgagg ctgtccgcga ccgccagcag
     1561 gtccgccagg acgcggcccg gcctgcgcca cgatcggcga cggtccgcca gggcgccagt
     1621 gaacgcggga aggatgccga aggccacccc gagcgcgtcc gacaggcctc gcagtttgcg
     1681 ccgctcgacg aaggcgagga tcgaggggtt gcgcagctcg gccgagatcg ccgtgaatcc
     1741 gacgatcacc atgaccgcgc gcagcgccat cgcggcgccc tccgccaggc cgccagcgag
     1801 tccgctcgcg ccgtggcgga cgagtccgaa cacgaggccg gcgagcagca tgacggcaag
     1861 cagctcgagc cagagcgaca ggcgccggat ccgccccgcc gcccgcgggt acaccgccag
     1921 cacccaggcc gcgaacaccg cgacgaccag cgcggcggcc gcgagaggca gcctggagag
     1981 gcccatcatg ccgccgacga gcacgaacgc cgctgcagcc aggcggcgca gcgaccaggg
     2041 cccttcggcc tcgatcggcg gctccgggaa gaccgcgggg gcggctgccg cgcccgggac
     2101 aagaggcgcc gccttgttgc cgacgcgtcc ggccacgcgg agaccgactg ccgccgccgc
     2161 cagccctgcc agccactcga ggacgacgaa cgtccagatg aggtcgaacg gaccgaagga
     2221 ctcgacggcc agggacctcg ccgcgaagcg gtacgcatcg acgtacaagc ggaccacgtc
     2281 gccgccgaag gcgatgatcg cggtgatgat ccgctgcgcc atcgaccacg agacggcggc
     2341 ggctcccccg atcaggtagc ccgcggcgcg cccccgcgcg agccggacgc cggcctcgag
     2401 caagaggccc tccatggcga tgcccaccat ggggcccagg atcaccgcgc tcggcgaaat
     2461 ggacttcatc agggcgcaga cgagcgccgc gcgccagatc aagccgcgct cgggatgcag
     2521 gcggtggccc gccgtcatca tcatgacgcc ggaggccgca aggagactgc ccgcgaacgg
     2581 gatgcgaagg ttgtggagga agctgccggc gacgatttcg gaggcggccc acaggctgcc
     2641 atagatcgcc gcacgctgcc acacccggcc cgagacgccg gccatcacgc cgtcagtcct
     2701 ccgccacccg cgccctgccg cgcgtggcgt tgagcagcgc ggagcggaac tcgccggccc
     2761 gccccgctgg cagacgcagc ctgacgcgca cgtcggcggc gaactcctgc cggaggattt
     2821 ccccttcgca ggcggcgcag gcctgctgca gcgcagagag gctggcgtag tccacgaccg
     2881 cggtcaacga caggtagtcg atgcgctcgg ccaccgccag cgccgccagc gcctgctgca
     2941 cgccgccgcc gtacgcgcgc acgaggccgc cggtgcccag cttcgttccg ccgtagtagc
     3001 gcgtgaccac cgccacgatg tcccccacgc cggcgcgcag cagcgcgttc agcatgggcc
     3061 tcccagccgt tccgtggggc tcgccgtcgt cgctcatacc aattcggctg gtgtcgccgg
     3121 gcgggcccac cacgtacgcc cagcagttgt gcgcggcgcc ggggtgctcc ccgctgacgg
     3181 cccggacaaa cgccgcagcc tcttcggccg tcgaggcgcg cccgaccgtc gtgatgaagc
     3241 ggctcctgag gatctcccgt tcggcgcgga tcgtcgccgc cggaacgaga taccgcgagg
     3301 ggagtggcat gtcgcctctc gggaactcgg cgtcgtgaaa gcgtacccca ttcctgccgt
     3361 tctggggtta aatagccgct ggagcacgct catcaccatg gagccgctgg acgatttccg
     3421 catgcagccg gaacaggagc cgccggtgcc ggacgccggc ggtccttcgt tcctgccgta
     3481 ctggattgcg gccgcggcgc tcgtgctggc ggtggccatc ggaggagcct ggtggtaccg
     3541 gcagcggccc tccgccgccg cgaaccaggc gccgtcccct gacggcgcag gtcaggccgc
     3601 cgagaccggc ggcgcccacg cgccgctggg gccggcggtc gaccccatcg aactgccccc
     3661 gctcgtgctc accgatcccc tcgtgcgcga cctgctgggt cagctgtctt cgagcccgac
     3721 cctggcttcg tggctcgcga ccgacggcct gatccggaag gccgtcgcgt cgattgagaa
     3781 tgtggccgag ggcgcgtcgc ccgcgacgca cgcccgcgtg ctcgcgccgc gcctcccgtt
     3841 cgccgcggag accgagggcg ggaccatcac gatcgacccg cgctcctacg cgaggtacga
     3901 cgggattgcg ggagccgtgg cgtctctgga tccggccggg gtggcgcgcg tgtactctct
     3961 gttgaagccc aggctggcgg aagcgtacgc cgacctcggc catcccgacg ccgacatcga
     4021 tgccgcagtc gaacgggccg tcagcatgct gctcggggcg ccggtcgtcg aggagcccgt
     4081 ggtcctccgc cggcgcgtgt tgtcctaccg ctacgatcgg gccgatttcg aggcgctgcc
     4141 ggccgcgcag aagcagctcc tgcgcatggg gccgcgcaat acgcgcatca tccaggagca
     4201 gctccgcgcc gtcgcccgcg aactgggcat tccggcgggg cggctgccgc cccgtcaaac
     4261 cgcccggtag gcgcgtggcg ccgccggccc gggagccgcg ggagaggcct ccacggtccg
     4321 tggatcccac gcgtcccgga cggcgtcgta gcagtgcaga cggcaggttc agcccatgac
     4381 gcgccttcaa ctcgcagcgg ccgctctctg cgcgctcgtc gcggccgtcg ttccctggcc
     4441 gcattcctgg agcaccggca cgatgtctca cgatccgtcc gccccgtcga agccattccc
     4501 gctcacgaag acggacgagg aatggcgggc ggcgttgacg ccgatgcagt tcgccgtcct
     4561 gcgccgcggc gacaccgagt atccgggcac gagcccgctc ctccacgagc acaagcccgg
     4621 ggtgttccgg tgcgcggcct gcggacaggc gctgttccac tccgccgcga agtacgagag
     4681 cggcagcgga tggccgagct tctacgacgc cgtcgaggac gccgtcggca cgcgccagga
     4741 ccgcgcgctg ggaatgctca gaacggaggt gcactgcagc cgttgcggag gccacctcgg
     4801 gcaccgcttt ccagacgggc ccgaaccgac cgggcaacgc tactgcatca acggggcggc
     4861 gctggcattc gaacccggcg cgccgtctcc cgagacgtga gtcgcttggc gcaccgggga
     4921 gcccgggggc cgcgaaggcc ggatcccggc ccgtcgaccg cggttccgtc cggcgtcacc
     4981 gcaggagctg gaagacctgg atcctgctgt tgtcccggtc tgcgacgaag acctcgtcgc
     5041 tgtcattgac gcagatctgc gaggggcgat tcagcgcccc ctcctcccgg cccatgacga
     5101 ccggcctcga gaggaacgaa ccgtcgcgcc cgaacccgac gatgctgccg cccgagccct
     5161 cgaggacgaa gatgaggccc ttgctgacgg caacgtgggc cggggacgag gcgagccacc
     5221 cggagaggtc gcctcccagc gaagagaacg cagccgcgcc tttcgccgcc gcatagagcc
     5281 gccgcccgac tgaatcgagc aggactaggg ccccggcctg gtcgatcgcg atgtcggtgc
     5341 cgaatcccgc gccctcggga accggaagcg cccgccggaa cctcccttcg ccgtcgatga
     5401 cgaggacgcg cgcggaaaag acgtcgagca cgtacagggc atccgacgcg tcaatcgcca
     5461 tcgccttggg aatgacggtg gcaggcggcg ggacgccgtc aaacgcgagg acgcctgccg
     5521 tcgtgccggc ggcatcgagg cgaacgacgc ggcgctgcct gccgtcgagc gcgtagatct
     5581 ccc
//
LOCUS       DAOSIF010000160         5569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13090_length_5569_cov_3.891911, whole genome shotgun sequence.
ACCESSION   DAOSIF010000160 DAOSIF010000000
VERSION     DAOSIF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13090_length_5569_cov_3.891911"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1839)
                     /locus_tag="PLT35_06735"
     CDS             complement(88..1839)
                     /locus_tag="PLT35_06735"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HOG28961.1"
                     /translation="MRRPLWLAASIVLCLAARPEAGPAAAPVLRLTSAEFAGGAAVRV
                     PLGELAGLTEARQLGAGARATSAAVRATFDLGATPGGPYAVWLKARAEGEGAPPAVEI
                     VLNGSRIPVGPRPFPREWWELRRFRLPAGALRPRANRLEVRCASAGGNRGVPQALLVA
                     EAVVGPVSHVPVHELTTAYTVDLSLASPAGPAPRPPTGGFAVRGTKGWGWTPEQYLSE
                     IPWLARFGLNFLMNGYTSMYTDAVRKINRWWEPIPDEKKRAFSEIARQCRLNGVEFCF
                     AFHPQLHSERPLKLDDEADFEALWRQYEWMQQAGVNWFSLCFDDIPTRGLDMPALAEG
                     QARVANRLLERLRRSSPSAQLVFCPVTYYGCGDAPSVRPYLEALGRTLLDDVYVFWTG
                     DQSVAPAITRACGETFRSLVRRRLFIWDNYPTNDRFPILHLGPVTGRDPDLTAVAGAY
                     LSNPHAPQHDVNRLPLATCADFAADPAGYDPQASIAEAIFALGRTAAQRDVLAALVEL
                     YPGELIYGRSEPNEVLILERFDRLLERPGGRGAAGALLSLAERTLDGLEREFPDWFES
                     TKTTLRAHVAHMQGRRR"
     gene            complement(1836..3596)
                     /locus_tag="PLT35_06740"
     CDS             complement(1836..3596)
                     /locus_tag="PLT35_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28962.1"
                     /translation="MTGIDWLVVAALCAASLSLGVWFTRSASRQGAAGFFTGNRSLPW
                     WAIGLSNSATYQSGVGGFVMLVLVFGLAGNWLWWTAWVVWMPLVAIVWAKLWRRLQVV
                     TTAELITLRYGGRPAAAARRVYAVLLFAYAVLIIGYITGFFAKTVAPLVPLSEWQILL
                     IFGGVTVAYTMFGGLAGVVFTDVLQFGVFLVGNLVFFAVAVPQFGGWEAILGRIESVR
                     PGGLDPLPPTDQIPLLTMLMLVVQGLFYASSPTAGEGMTAQRFMGAVNERHAMGGQLF
                     NAFLSLSLRTIPLIGLGTIALSLFWTADLAGAIGPAPAGMTTLADPAHAWGTLVRVTA
                     LPLGLAGLLVATEAAAFMSTLSSLINWGSSLVVNDFYLPARPDAPRRRQIVVSRLTTL
                     ALFAFAGTVAILFVKGMVGWFLFINSAMVVFLLPLSWLRFFWWRFNVWGELAAIVLGL
                     PASILVWFVWGFEHRPLWQGTGILFLTSVAVLLTVTLLTPAESDDTLVAFYRRCRPPG
                     RWAHIARRAGAASAPPESWPKLVVDCLLGVVACVGLVVATNAAFVARWLPFGAGLAGG
                     LAAGTWLLRRIWPGREGGAT"
     gene            3718..4680
                     /locus_tag="PLT35_06745"
     CDS             3718..4680
                     /locus_tag="PLT35_06745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG28963.1"
                     /translation="MDRREFITRSSAASLLACFPASLAGIERTFEAGKLERRALGRTG
                     ERLSLIGFGGIVVKDATTAEAAARVAEAIDHGVNYFDVAPTYGNAQDMLGPALEPYRK
                     GVFLACKTTERGRAGSQRELDESLRKLRTDRFDLYQLHAVNTREDVERVFMADGALET
                     LVAARKAGKVRFLGFSAHTVEAALALIARFDFDTILFPINFATWHAGSFGPQVLAAAQ
                     AKQMGILALKAMAKGPWPAGAERDQHPKCWYEPLSDPADAMMGLRFTLSHPVTAAVPP
                     GDERLFKMALGLGLRFTPLAAQDAAAIKERGQKTAPLFTHPNWT"
     gene            complement(4712..>5569)
                     /locus_tag="PLT35_06750"
     CDS             complement(4712..>5569)
                     /locus_tag="PLT35_06750"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOG28964.1"
                     /translation="QGDGWQSSASVQFRNFWRSSVLLSYAKRVWDDKLTRGGPTVIRP
                     GNRGVTVSTSSDTRRRLAFSAAYAFTDRDYDAESHASELQLVWRPAPALTVTAGPAIT
                     HSIVAAQYLATVPDALATRTFGARYVFGEMRQAQVSITTRVNLVASPRTSLQVYLQPL
                     VSAADYGAIKEVYAPRTFDFVRYGEDAGSIVDAGSRYTIDPDGAGGASPFSIAKPDFN
                     LRSLKANAIFRWEFRPGSSLFVVWTQQRRDRAPDGTFDFGADAKRWFTARPDNVFLVK
                     LSYWFGIDR"
BASE COUNT          929 a         1978 c         1983 g          679 t
ORIGIN      
        1 cggcgtctcg gcgaagctgg ggccgacaag cacggcccag tcccccgcgt agtagatgcg
       61 agccatcggg tcgcctcctt cgcgccgcta tcggcggcgc ccctgcatgt gcgccacgtg
      121 cgcgcgcagg gtcgttttgg tggactcgaa ccagtccggg aattcccgct cgaggccgtc
      181 gagcgtacgc tcggccagcg acagcagcgc gcctgcggcg ccacggccgc cgggccgctc
      241 cagcaggcgg tcgaaccgct cgaggatgag cacttcgttg ggctcggaac ggccgtagat
      301 caactctccg ggatacagct cgacgagtgc cgcgaggaca tcccgctggg ccgcggtgcg
      361 gccgagggcg aagatggcct ccgcgatcga cgcctgcggg tcgtagcctg ccgggtcagc
      421 agcgaagtcg gcgcacgtgg ccagcggcag gcggttcacg tcgtgctggg gcgcatgcgg
      481 gttgctgaga taggcgccgg ccaccgccgt cagatcggga tcccggccgg tcaccggccc
      541 gaggtgcaag atgggaaacc ggtcgttcgt ggggtagttg tcccagatga agagccggcg
      601 gcgcaccaac gagcggaacg tctcgccgca cgcgcgggtg atcgccgggg ccaccgactg
      661 gtctccggtc cagaacacgt acacgtcgtc gagcagcgtc cgccccagcg cttcgaggta
      721 cggcctgacc gaaggagcgt ccccgcagcc gtagtaggtg accgggcaga acacgagctg
      781 ggccgacggc gaggaccgcc tgagccgctc gaggaggcgg ttcgcgacgc gggcctgccc
      841 ctcggcgagc gccggcatgt ccaggccccg cgtggggatg tcgtcgaagc acaagctgaa
      901 ccaattgacc ccggcctgct gcatccactc gtactggcgc cacagcgcct cgaagtccgc
      961 ctcgtcgtcc agcttcagcg gccgctcgga gtggagctgg gggtggaagg cgaagcagaa
     1021 ctccacgccg ttcagccggc actggcgcgc gatttccgag aaggcgcgct tcttctcgtc
     1081 cgggatcggc tcccaccatc ggttgatctt cctgacggcg tcggtgtaca tcgacgtgta
     1141 gccgttcatc aggaagttca agccgaaccg cgcgagccac gggatctcgg acaggtactg
     1201 ctcgggcgtc catccccagc ccttggtgcc ccggacggcg aagccgccgg tcgggggccg
     1261 cggcgccggg ccggccggcg aggccagcga cagatccacg gtgtaggccg tggtcagctc
     1321 gtgaaccggc acgtgcgaca ccgggccgac aacggcctcg gcgacgagca gcgcctgcgg
     1381 cacgccgcga ttgccgcctg cgctcgcgca ccgcacctcg agccggttcg cgcgcggacg
     1441 gagcgcgccc gccggcagtc ggaaccgccg aagttcccac cactcgcggg ggaagggccg
     1501 aggcccgacg gggatccggc tcccgttcag cacgatctcg acggctggcg gagcgccctc
     1561 tccctcggcg cgggctttca gccagacggc gtaggggccg cccggcgtgg cgccgaggtc
     1621 gaaggtcgcg cggactgcgg cgcttgtcgc gcgcgcgccg gcgccgagct gacgagcctc
     1681 ggtcagcccc gcgagttcgc ccagcggcac ccgcaccgcc gccccgccgg cgaactcggc
     1741 gctcgtcagc cgcaggaccg gagcggcggc gggcccggcc tccggcctgg ccgcgagaca
     1801 cagcacgatc gacgcggcaa gccacaacgg ccgcctcatg tcgctcctcc ctcccgcccg
     1861 ggccagatgc gccgcagcag ccaggtgccg gccgcgaggc cgccagccaa acccgccccg
     1921 aacggcagcc acctcgccac gaaggcggcg ttcgtcgcca cgaccaggcc gacgcacgcg
     1981 accaccccga gcaggcaatc gaccaccagc ttcggccacg actccggcgg ggcggaagcc
     2041 gcgcccgcgc gccgcgcgat gtgcgcccat cgcccggggg gtctgcacct gcggtagaac
     2101 gccaccagcg tgtcgtcgga ctcagccggt gtgagcagcg tgacggtcag aagcacggcg
     2161 acgctcgtca ggaacaggat gcccgtcccc tgccacaacg ggcggtgctc gaagccccac
     2221 acgaaccaca ccaggatcga cgccggcagc ccgaggacga tcgcggccag ctcgccccac
     2281 acgttgaaac gccaccagaa gaagcggagc cacgacaggg gcagcaggaa gacgaccatc
     2341 gccgagttga tgaagagaaa ccagcccacc atgcccttga cgaacaggat cgccacggtc
     2401 cccgcgaacg cgaagagcgc gagcgtcgtc aggcggctga ccacgatctg acggcgccgt
     2461 ggcgcatcgg gccgcgccgg caggtagaaa tcgttcacga cgaggctgct gccccaattg
     2521 atcagcgagc tcagggtgga catgaaggcg gccgcctccg tcgccacgag cagccccgcc
     2581 aggccgagcg gcagcgccgt gacgcgaacc agcgtgcccc acgcgtgcgc cgggtccgcc
     2641 aaggtcgtca tgcctgcggg ggccgggccg atggcgccgg ccagatcggc ggtccagaac
     2701 agcgacagcg cgatcgtgcc cagcccgatg agcgggatgg tccgcagcga caacgagagg
     2761 aacgcgttga agagctgccc gcccatcgcg tggcgctcgt tgacggcgcc catgaaccgc
     2821 tgggcggtca tcccttcgcc cgccgtcggc gaactggcgt agaagagccc ctgcacgacg
     2881 agcatcagca tggtcagcag cgggatctgg tcggtcggcg gcagcggatc caggccgccg
     2941 ggcctgaccg actcgatgcg gccgagaatc gcctcccatc cgccgaattg cgggaccgcc
     3001 acggcgaaga acacgaggtt gccgacgagg aagacgccga actgcagcac gtcggtgaag
     3061 accaccccgg cgaggccgcc gaacatcgtg taggccaccg tgacgccgcc gaagatcagg
     3121 aggatctgcc actcgctcag gggcacgagc ggcgccaccg tcttcgcgaa gaacccggtg
     3181 atgtagccga tgatcaggac cgcgtaggcg aacagcagca cggcgtacac gcggcgcgcg
     3241 gcggcggcag gtctcccccc gtaacgcagc gtgatcagct cggccgtcgt cacgacctgc
     3301 aggcggcgcc acagcttcgc ccagacgatg gcgacgagcg gcatccagac gacccaggcc
     3361 gtccaccaga gccagttgcc ggcgaggccg aacaccagga cgagcatgac gaagccgccg
     3421 acgcccgact gataggtggc ggagttggac aggccgatcg cccaccacgg gaggctccgg
     3481 ttgccggtga aaaaacccgc cgcaccctgg cgggaggcgg agcgggtgaa ccagacgccg
     3541 agggacagcg aagccgcgca cagggcggcg acgacgagcc agtcgatgcc ggtcatgcgg
     3601 gaatcccgac cgggggcgcg ctccggagcg gcgaggccgc ggtcccgggc cgaagaaccg
     3661 cagctagtat atgatgcccc ccgccaggca gcgaaggcct cgcgggaagg agtgcccatg
     3721 gaccgccgcg agttcatcac gaggagttcc gccgcgtccc tgctcgcctg cttccccgcc
     3781 tccctggccg ggatcgaacg gacgttcgag gccggcaagc tcgagcgccg cgcgctcggc
     3841 cggacgggcg agcgcctctc cctcatcggt ttcggcggca tcgtcgtcaa ggacgcgacc
     3901 acggcggagg ctgccgcccg cgtggcggag gcgatcgacc acggcgtcaa ctacttcgac
     3961 gtggcgccga cctacggcaa cgcgcaggac atgctcgggc ccgcgctgga gccgtaccgg
     4021 aagggcgtgt tcctcgcgtg caagacgacg gagcgcggcc gggcgggctc gcagcgggag
     4081 ctggacgagt cgctgcgcaa gctccggacg gatcgcttcg atctctacca gctgcacgcg
     4141 gtcaacacgc gcgaggacgt cgagcgggtc ttcatggccg acggggcgct cgagacgctg
     4201 gtggcggcca ggaaggccgg gaaggtccgg ttcctcggct tctcggccca cacggtcgaa
     4261 gcggcactgg cgctcatcgc gaggttcgac ttcgacacga tcctcttccc catcaacttc
     4321 gcgacgtggc acgccgggag cttcgggccg caggtgctcg ccgcggcgca ggcgaagcag
     4381 atgggcatcc tcgcgctcaa ggccatggcg aaaggcccct ggcccgccgg cgcggagcgg
     4441 gatcagcacc cgaagtgctg gtacgagccg ctgtcggacc cggctgacgc gatgatgggc
     4501 ctgcgcttca cgctgtcgca cccggtgacg gccgcagtgc cgccgggcga cgagcgcctg
     4561 ttcaagatgg ccctcgggct gggactgcgc ttcacgccgc tcgcggcgca ggacgcggcc
     4621 gcgatcaagg agcgggggca gaagacggcg ccgctcttca cgcacccgaa ctggacctag
     4681 cagggggccg gacgagccgc ccgcccggcc gtcagcggtc gatgccgaac cagtagctca
     4741 gcttgaccag gaacacgttg tcggggcgcg cggtgaacca gcgcttcgcg tcggccccga
     4801 agtcgaacgt cccgtcgggc gcgcggtcgc gccgctgctg cgtccacacg acgaagagcg
     4861 aggacccggg ccggaactcc caccggaaga tggcgtttgc cttgagcgac cgcaggttga
     4921 agtcgggctt ggcgatcgaa aaggggctcg cgccgccggc gccgtcggga tcgatcgtgt
     4981 agcgcgagcc cgcgtcgaca atcgagcccg cgtcctcccc gtaccgcacg aagtcgaacg
     5041 tgcgcggcgc gtacacctcc ttgatggcgc cgtagtccgc cgccgacacg agcggctgca
     5101 ggtacacctg cagcgaggtc cgcggcgacg caacgaggtt gacccgcgtc gtgatcgaca
     5161 cctgcgcctg tcgcatctcg ccgaacacgt agcgcgcccc gaacgtgcgc gtggccagcg
     5221 catcgggcac ggtggccaga tactgggccg ccacgatgct gtgcgtgatg gccggaccgg
     5281 ccgtcaccgt cagggcgggg gccgggcgcc agacaagctg cagttccgac gcatgcgact
     5341 cggcgtcgta gtcccggtcc gtgaaggcgt aggcggccga gaaggccagg cgccgccggg
     5401 tgtcgctgga cgtggacacc gtcacgccgc ggttgccggg ccggatgacc gtcgggcctc
     5461 cccgggtcag cttgtcgtcc cagacccgct tcgcgtacga gagcagcacg gacgaccgcc
     5521 agaagttccg gaactggacg ctcgctgagg actgccagcc gtcgccctg
//
LOCUS       DAOSIF010000161         5555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13131_length_5555_cov_3.246364, whole genome shotgun sequence.
ACCESSION   DAOSIF010000161 DAOSIF010000000
VERSION     DAOSIF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13131_length_5555_cov_3.246364"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>217)
                     /locus_tag="PLT35_06755"
     CDS             complement(<1..>217)
                     /locus_tag="PLT35_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28965.1"
                     /translation="KGDGNAGIRIGSGVFIGRNTILSCKNGDIEIGDGANIGFNCEVF
                     SASRVEIGPRSLMAAYCYVIGGDHEYAD"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<228..519)
                     /locus_tag="PLT35_06760"
     CDS             complement(<228..519)
                     /locus_tag="PLT35_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28966.1"
                     /translation="MADIPKAQDQLFDERTSAREKYVRFVVGKPGLAALLTHEAIVLL
                     CQATPGALGLALRKLLYPRLLGACGRNVVFGQNVVLRHPGKIRVADNVVIDDN"
     gene            complement(519..1502)
                     /locus_tag="PLT35_06765"
     CDS             complement(519..1502)
                     /locus_tag="PLT35_06765"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG28967.1"
                     /translation="MRTLVTGAAGFTGGHLARALRAGGHEVRALVRAADARAAALARD
                     GIDVATGDLRDPGALGRAVDGVEVVYHVAALYRQASARRADYRAVNALAVAALADAGA
                     SAGVRRIVYCSTVGVHGDVERPPAGEDAPLRPGDEYQRSKLEGERLGREAAARRGLEF
                     VVARPSGIYGPGDRRLLKLFRGVARRRFVILGSGAIFYHLTYIDDLVEGLRLCGRVPE
                     AAGRTYILAGGEVTSLSELVGRIARQAGVKPLPLRLPVWPVWAAGAVCEAICVPLGVE
                     PPLYRRRVDFFTKSRAFDISRARAELGFAPQVGLDEGIGRTLDWYRKYGWV"
     gene            complement(1490..2233)
                     /locus_tag="PLT35_06770"
     CDS             complement(1490..2233)
                     /locus_tag="PLT35_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG28968.1"
                     /translation="MTKDHYSYRVYADPSMADAFDAMRFSGPIGTMLAETQDRVLAEF
                     LGPVDGRGVVDVGTGTGRAALGLARRGARVTGVDASEEMLRVARARAAEARVEATFEA
                     GDAHALPYPDRAFAAAVSLRVLMHTPDWRACLRELCRVADERVVVDFPAACSAAALQA
                     AFRRAASALGRPVEAYRVLREREVESVLASCGFRVAGWHRQFVLPIAFHKLVGSRSFT
                     RASERVLACLGLLRLAGSPVTVVALRCER"
     gene            complement(2230..3420)
                     /locus_tag="PLT35_06775"
     CDS             complement(2230..3420)
                     /locus_tag="PLT35_06775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG28969.1"
                     /translation="MRILMIAPEPFFEARGTPFSEYHRIRALIDLGHTVDLVTYPFGR
                     DVALAGLRIHRSWRPPFVRGVKVGPSFAKLPLDALLAAKALHVALKWRFDYVHSHEEG
                     GGIGLVLAWLRNVPHLYDMHSSLPEQLANFGFSRSKVLIAVFRALERRMIRRSASVIV
                     ICRQLEETARAIAPGAHVVLIENAPGSAETAGAASRVRVRETAGLASGVPLVVYTGTF
                     EAYQGLDLLFAAMRTVLASRPDARLLLVGGKPNQVARARVDAERAGVGHAAVFAGERP
                     GDEIPSYLEAADVLVSPRSRGTNTPLKLYQYLRAGRPIVATNLLTHTQVLDARVAELT
                     DPTPEAFGAGILRVLGDREYALALGRAAGDLAATRYTYESYVARTREALAPIAARAAA
                     GAQP"
     gene            3622..4653
                     /locus_tag="PLT35_06780"
     CDS             3622..4653
                     /locus_tag="PLT35_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG28970.1"
                     /translation="MADRNDVGGPGPLRRALMLLLKLAVSAGLLGLLLARTDLSRLWL
                     SARQASPLWLALALVLYLAMILLSGWRWKLLLDAQHVRVAFGRLVNSYLVAVFFNNFL
                     PSNIGGDVIRIKDTAPQAGSKTLATTIVLMDRGLGLLGLLAVAAFGASVTGRMGGRPP
                     ILASMLWLALAGGVAVAAVAVLLPGGVGRLLSPLRLIHREWIDERIKRLTGALARFRG
                     APGALATCLAGAVLVQAVLVAFYAAVVRSMDIPLSPWHLAVIVPVSFVVQMAPVSLNG
                     FGVREATFAYYFSRLGLSIESSLAVSFMGAGLVILFSLSGAAAYWCRGSRWVLEGASA
                     GVPPAAGSR"
     gene            4816..>5555
                     /locus_tag="PLT35_06785"
     CDS             4816..>5555
                     /locus_tag="PLT35_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28971.1"
                     /translation="MMSRRVFGVLLIPVTMAAVAACEAVKSATPTSPSVAGPIAGVNI
                     TNPHPVAPPQGTTIASTDQPVTLTVNNVTTNGQRPVTYLFEVASDSEFATKVVTREGI
                     EPGADGKTSYRLPDALTPERTYFWRAKAKDGANESGYSVVLSFNVVTPVIIQAPVPIS
                     PVSGATISTLRPTFKTNNAARSGPVGAVTYVFDIARNATFSDKEAVVTVSEQAGQTKF
                     TIDQELVPDKKYYWRVKAYDPQVTSEWSL"
BASE COUNT          839 a         1981 c         1899 g          826 t
ORIGIN      
        1 gtcagcatac tcgtgatcgc cgccgatgac gtagcagtag gcggccatca ggctccgggg
       61 gccaatctcc acgcggctgg ccgagaagac ctcgcagttg aacccgatgt tggcgccgtc
      121 gccgatctcg atgtctccgt tcttgcacga caggatggtg ttgcgcccga tgaagacccc
      181 cgatccgatc cggatgcctg cgttcccgtc ccccttcnnn nnnnnnnagt tgtcgtcgat
      241 gacgacgttg tcggccaccc ggatcttgcc cgggtggcgc aggacgacgt tctggccgaa
      301 gacaacgttc cggccgcacg cgcccagcag gcgcggatac agcagcttcc tgagagccag
      361 gccgagggcg cccggcgtgg cctggcacag gagcacgatc gcctcgtgcg tcagcagggc
      421 ggccaggccc ggctttccga cgacgaagcg cacgtacttc tcgcgcgccg acgtccgctc
      481 gtcgaacagc tggtcctggg ccttcgggat gtcggccatc acacccaccc gtacttgcgg
      541 taccaatcca gcgtgcgccc gatgccctcg tccaggccga cctgcggcgc gaagcccagc
      601 tcggcgcggg cgcggctgat gtcgaaggcg cggctcttcg tgaagaaatc gacgcgcctg
      661 cggtagagcg gcggctcgac tcccagcggc acgcaaatcg cttcgcacac ggcgccggcc
      721 gcccagaccg gccacaccgg caggcggagc ggcaggggct tgacgcctgc ctgtcgcgcg
      781 attcggccga ccagctcgct caggctggtg acttccccgc ccgccagaat atacgtccgt
      841 ccggccgcct ccggcacgcg gccgcagagg cggaggccct cgactaaatc gtcgatgtac
      901 gtgaggtgat agaagatcgc gccggagccg agaatgacaa acctccgccg cgcgacgccc
      961 cggaacagct tgaggagcct ccggtctccc ggcccgtaga tcccgctcgg acgggcgacg
     1021 acgaactcga ggccgcgccg ggcggcggcc tcccgcccga ggcgctcccc ttccagcttc
     1081 gatcgctggt actcgtcgcc gggcctgagc ggcgcatcct cgccggcagg cgggcgctcg
     1141 acgtcgccgt gcacgccgac ggtgctgcag tacacgatgc gccgcacgcc cgccgaggcg
     1201 cccgcatccg ccagggcggc cacggccagg gcgttgacgg cgcggtagtc cgcccggcgc
     1261 gccgaggctt gccggtagag cgccgcgacg tggtacacga cctcgacgcc gtcgaccgcg
     1321 cggccgaggg cgcccgggtc gcgcaggtca cccgtcgcga cgtcgatgcc gtcgcgcgcc
     1381 agcgcggccg cgcgcgcgtc ggcggcgcgg acgagcgccc gcacctcgtg cccgcccgcc
     1441 cgcagcgccc gggccaggtg cccgccggtg aatcccgcgg cgccggtgac tagcgttcgc
     1501 atcggagcgc caccacggtc accggcgacc ctgccagcct cagcaggccg aggcaggcga
     1561 gcacgcgctc ggacgcacgc gtgaacgatc tcgacccgac gagcttgtgg aacgcgatgg
     1621 gcagcacgaa ctggcggtgc cagccggcga cgcggaagcc gcaggacgcg agcaccgact
     1681 cgacctcccg ctcccttagg acgcggtagg cctccaccgg gcggccgagg gccgatgcgg
     1741 cccgccggaa cgccgcctgc agcgccgccg cgctgcaggc ggccgggaag tcgacgacga
     1801 cgcgctcgtc ggccacccgg cacagctcgc gcaggcaggc gcgccagtcg ggcgtgtgca
     1861 tcaggacgcg caggctcacc gccgcggcga acgctcgatc ggggtacggc agggcatggg
     1921 cgtcgccggc ttcgaacgtc gcctccacgc gcgcctcggc ggccctggcg cgcgccacgc
     1981 gcagcatttc ttccgacgca tcgacgcccg tcacgcgcgc cccccggcga gccaggccca
     2041 gcgccgccct ccccgtgccc gtgccgacat cgacgacgcc gcgcccgtcg accggaccga
     2101 ggaactcggc gagcacgcgg tcctgggttt cggcgagcat cgtgccgatg gggccgctga
     2161 atcgcatcgc gtcgaacgcg tccgccatcg acgggtccgc gtagacccgg tagctgtagt
     2221 ggtccttcgt cacggctgcg cccccgcggc agcgcgcgcg gcgatcggcg cgagcgcttc
     2281 cctcgtgcgc gcgacgtacg actcgtaggt gtagcgtgtc gccgcgaggt ccccggccgc
     2341 ccgcccgagc gccaaggcgt actcccggtc gcccagcacg cggaggatgc ccgcgccgaa
     2401 agcctccggc gtcgggtcgg tcagctcggc gacgcgggcg tcgagcacct gcgtatgcgt
     2461 cagcaggttc gtcgcgacaa tcgggcgccc ggcccggagg tactggtaga gcttcagcgg
     2521 cgtgttggtg ccgcggctgc ggggggaaac caggacgtcg gccgcctcga gatacgacgg
     2581 gatctcgtcg ccgggccgtt ctcccgcgaa caccgccgcg tgtccgacgc cggcgcgctc
     2641 ggcgtccacc cgcgcccgcg cgacctggtt cggcttccct cccaccagaa gcagcctggc
     2701 atcgggccgc gaggccagga ccgttcgcat cgccgcaaac aacaggtcga ggccctggta
     2761 ggcctcgaag gtccctgtgt agaccaccag gggaacgcct gaagcgagcc ccgctgtttc
     2821 acggacccgc acccgggagg cggctcccgc tgtctcggcc gaaccaggcg cgttctcgat
     2881 gagcacgacg tgcgcgccgg gcgcaatcgc gcgggccgtc tcctcgagct gcctgcagat
     2941 gacgatgacg gaagcggagc ggcggatcat tcgccgctcg agcgcccgga aaacggcgat
     3001 gaggaccttc gagcggctga acccgaagtt cgcgagttgc tcggggaggc tcgagtgcat
     3061 gtcgtagagg tgcggcacgt tgcggagcca cgccagcacg agcccgatgc cgcctccctc
     3121 ttcgtgcgaa tggacgtagt cgaagcgcca cttgagcgcg acgtgcaggg ccttggcagc
     3181 gagcagcgcg tccagcggca gcttggcgaa ggaggggccg accttcacac cccgcacgaa
     3241 cggcgggcgc caggatcggt ggatgcggag ccctgcaagc gcgacatcgc ggccgaacgg
     3301 atacgtgacc aggtccaccg tgtgcccaag atcgatcagc gcccgaatcc ggtggtactc
     3361 gctgaacggg gtgccccgcg cctcgaagaa cggctcggga gcgatcatca ggattcgcac
     3421 ggccctgtca ttctaggtgt cccgctcgag catcgtcaat gaacggcctc gcccgtgggg
     3481 ccaaggcgca aggccgattc gagcccgttt acagggttcc agccacgtgc taggcttcag
     3541 attgccttgg ggggcaccct cctcttccgg ctcgctcggg cccccggccc gaacagctga
     3601 cgcgtggagc agcagcgaaa catggcggac cgaaacgacg taggcgggcc cggaccgctc
     3661 cggcgcgcgc tgatgctcct gctgaagctc gcggtcagcg ccgggctgct cggcctgctg
     3721 ctcgcgagga ccgacctgtc gcgcctctgg ctgagcgcga ggcaggcctc gccgctctgg
     3781 ctcgcgctgg cgctcgtgct ctatctcgcg atgatcctgc tgagcggctg gcggtggaag
     3841 ctgctgctgg atgcgcagca cgtgcgcgtg gcgttcgggc gcctcgtgaa ttcctacctc
     3901 gtcgccgtct ttttcaacaa cttcctgccg agcaacatcg gcggcgacgt catccggatc
     3961 aaggacaccg cgccgcaggc gggatccaag acgctggcga cgacaatcgt gctgatggat
     4021 cgcggcctcg ggcttctggg cctgctcgcc gttgccgcct tcggggccag cgtgacgggc
     4081 aggatgggag ggcggccccc gatcctggcg tcgatgctct ggctggcgct cgccggaggt
     4141 gtggcagtcg cagccgtcgc ggtcctgctg cccggaggcg tcggccgcct gctcagcccg
     4201 ctccggctca tccaccggga atggatcgac gagcgcatca agcgcctgac cggcgcgctc
     4261 gcgaggttcc gaggcgcacc gggggcgctg gcgacctgcc tggccggcgc agtcctggtg
     4321 caggccgtcc tggtcgcctt ttacgccgcc gtcgtccgaa gcatggacat cccgctctcg
     4381 ccgtggcacc tggccgtcat cgtcccggtg tccttcgtcg tgcagatggc gcccgtgtcg
     4441 ctgaacggat tcggcgttcg cgaagccacg ttcgcgtact acttctccag actgggcctg
     4501 tccatcgagt cgtcgctggc ggtctcgttc atgggtgctg gcctcgtcat cctgttctcg
     4561 ctttcaggag ccgccgccta ctggtgtcgc ggctcccgat gggtcctcga gggggcctcc
     4621 gcaggcgtgc cgccggctgc cggctcacga tagccggccc tgacgttcgc cgtgtgaccc
     4681 cgtgaagtgc gaaatccgtt cgtccgatac cccggaagca ggagaccctg tccgcgagtg
     4741 tggcacgggg gttgaaaagg ccattctgga agctgtcccg cagcggcttt cccgggctgc
     4801 gtccacggag cgctcatgat gagtagacga gtcttcggcg tcctgttgat cccggtgacg
     4861 atggcggccg tcgccgcctg cgaggcggtg aagagcgcca cgcccacgag tccgtctgtg
     4921 gcgggtccga tcgcgggcgt caacatcacg aaccctcacc cggtggcgcc cccgcaggga
     4981 accaccatcg cgtcgacgga tcagcccgtt acgttgaccg tgaacaacgt cacgacaaac
     5041 ggccaacggc cagtcactta cctgttcgag gtcgcctcgg actcggagtt cgccaccaag
     5101 gtcgtcaccc gcgaaggcat cgagcctgga gccgatggca agacgagtta tcggctcccg
     5161 gacgcgctga cgcctgaacg gacgtatttc tggcgggcca aagccaagga cggcgcgaac
     5221 gagagcgggt actccgtggt cctcagtttc aatgtggtca ccccggtcat catccaggct
     5281 cccgtgccga tctccccggt gagcggagcc acgatctcca cgctgcggcc gacgttcaaa
     5341 accaacaacg ccgcgcgcag cggcccggtt ggtgcggtca cttacgtttt tgacattgct
     5401 cgcaatgcga ccttcagtga caaagaggcg gtcgtcacag tctccgagca ggccggccag
     5461 accaagttca cgattgacca ggaactggtg ccggataaga agtactactg gcgggtgaag
     5521 gcctacgacc cgcaggtcac gagcgaatgg tcact
//
LOCUS       DAOSIF010000162         5535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13180_length_5535_cov_4.922810, whole genome shotgun sequence.
ACCESSION   DAOSIF010000162 DAOSIF010000000
VERSION     DAOSIF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13180_length_5535_cov_4.922810"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLT35_06790"
     CDS             complement(<1..563)
                     /locus_tag="PLT35_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28972.1"
                     /translation="MSVVEMTAVPAPAAPRDVAESGLNVDLLTQLVLKTLHLAGTLTG
                     LELAARLGLRFPVLEPSLDDLKWQHHVEVVAGAAIGGAGYKYRITDAGRERAALFLAH
                     NRYVGVAPVPLDQYRRYLERFSRNVVNDVSPERVREAFRTLVLSDSVLDQLGPAIRAG
                     RSLFVYGPPGNGKTVISQCIRNLLCGE"
     gene            complement(560..844)
                     /locus_tag="PLT35_06795"
     CDS             complement(560..844)
                     /locus_tag="PLT35_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28973.1"
                     /translation="MKQACLGLLSETMLAGRGAEHLRTRIRPAAPAAPGGAGTAERYP
                     VDLAAALASRASAPTGAGEIVPAMACVRAGVPGHARARFVPAAPEMGEVP"
     gene            complement(1031..1945)
                     /locus_tag="PLT35_06800"
     CDS             complement(1031..1945)
                     /locus_tag="PLT35_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG28974.1"
                     /translation="MPVEFVVALGAVFVSVALLAGLAASAILSKQSAAQRRLDAILPS
                     RARTEVLLQGPLTSERDPKLARWSRLLPKSAQDMSGLEQRLAAAGIKSPLAAIAFGSA
                     EIALPVLAVVGVFSFLGLSDRAGWVAALFAGALGYIAPGFVLDSMIGARRKQIRNGLP
                     DLLDLLIVCLEAGSSLDQAVIKASEELGIAYPALAEELRILSTETRAGMARLEAFRNL
                     AQRTRVEDVRALVAMLVQTDRFGTSVAQTLRTMADDMRLKRRQAAEEMAAKVGVKLVF
                     PLVFFLFPALYVVVLGPAVIQFLRNFPD"
     gene            complement(2048..3010)
                     /locus_tag="PLT35_06805"
     CDS             complement(2048..3010)
                     /locus_tag="PLT35_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG28975.1"
                     /translation="MAFAALTFALVVVIVLGTYWALIERPARAAELALRRRLGAAKAG
                     ARVSSSVQSEARRLSSLPALEQLLARRMNLTGPIVRLIDQSGLKTTVGVVLLSAGCLF
                     MLGLLVGQTWSGSLQVGLVLGACLAAAPVLFLRYKGAQRVRRFEELFPEALDLMTRAL
                     RAGHTFAAALGMVADELPEPIAAEFKLLHDQQNFGMPLPEALRSFGERVPLLAAKFFV
                     TAILTQRESGGNLTEVLTNLASVIRDRFMVKRQVQVRSAHGRVTGWILVGLPPALAVI
                     FSIINPKHFAAMLDERLGIQMIVGAVVFQVVGALVIRKIVNVDY"
     gene            complement(3026..4348)
                     /locus_tag="PLT35_06810"
     CDS             complement(3026..4348)
                     /locus_tag="PLT35_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOG28976.1"
                     /translation="MTPVPPAVPRVDTRHPQYQELKSRVHQGLLSRLNLERLTRVSRN
                     EAEPEIRGVIMGMLDAESDQVPLSLFERQSLIGDVLNELFGLGPLEELFADQEVSDIL
                     VNRFDQVYVERHGVLEPVDVVFKDDQHLMRIIERIVSSVGRRIDESSPMVDARLADGS
                     RVNAIIPPLALDGPALSIRRFRTDRLGAQDLVERDSLTQPMLDFIRAAVACRTNVIVS
                     GGTGAGKTTLLNVLSGFISDRERIVTVEDAAELVLRQRHVVRLETRPPNIEGKGAIRQ
                     RQLVINALRMRPDRIIVGEVRGDEALDMLQAMNTGHDGSLTTIHANSARDALYRLDTM
                     VAMANLNIPERAIRQQVASAVNLVVHMSRMADGTRKVTSVTELTGMEGEVISMQDIFV
                     FERLGINQQGRVCGRFRATGIRPKMSERLTTAGHVLPADMFEHVKNIT"
     gene            complement(4368..>5535)
                     /locus_tag="PLT35_06815"
     CDS             complement(4368..>5535)
                     /locus_tag="PLT35_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28977.1"
                     /translation="RGSRRALQRHIAFVGAGDRQVEQLLRARGLTVLTWSERDLSAKA
                     QQASAVADVVMLDLRGEPQRTLPAGLDAFRRQHPAIPILLLVSALDPALMLEAMRAGV
                     SECLQDPLAGDDLDAALGRIAASRDAGPAGDVFAFLGAKGGVGTTTAAVNVATELSRL
                     RAGSVLLVDLHLAYGDAAIYLAAEPRFSVADALENTHRLDAAFLKSLCVKTKAGPELL
                     ASPDRALGLLVDAGRVRALVDSAARHYRYVVLDLCRTDPAVLDGLDPVKKILVIANQE
                     LATVRGASRMAETLNRRYGKDRVVLAISRYDPNAGIGQEDIERVVGSKIRHLLPSDYR
                     VALEALNTGRPLALSDQTGLAGALKELARELADLPAEEPQRGGRSIFGRLTGRS"
BASE COUNT          830 a         1965 c         1943 g          797 t
ORIGIN      
        1 atctccccgc agagcaggtt ccggatgcac tgcgagatca cggtcttgcc gtttccgggc
       61 gggccgtaga caaacagcga ccgcccggcg cggatcgccg gtccgagctg gtcgagcacg
      121 ctgtcgctca gcaccagcgt ccggaacgcc tcccgcacgc gctccggcga gacgtcgttc
      181 acgacgttcc gggagaaacg ctcgaggtag cggcggtact gatcgagcgg caccggcgcc
      241 acgccgacgt agcggttgtg cgcgaggaag agcgccgcgc gctcgcgtcc ggcgtcggta
      301 atccggtact tgtagcccgc cccgccgatt gccgccccgg cgacgacctc cacgtggtgc
      361 tgccacttca ggtcgtccag gctcggctcg agcacgggaa accggaggcc caggcgcgcg
      421 gccagctcca ggccggtcag cgtgcccgcc aggtgcagcg tcttcagcac cagctgcgtc
      481 aggagatcga cgttcagtcc cgattccgcg acgtcgcgcg gagctgcggg cgccggcacc
      541 gccgtcattt caacgacgct catgggacct cccccatttc gggcgcagcg gggacgaagc
      601 gcgcgcgggc gtggccagga acgcccgcgc gcacgcaggc catggccggc acgatctcgc
      661 cggcgccggt cggcgcactt gcgcggctgg caagggccgc ggcgagatca accgggtacc
      721 gctccgccgt cccggcgccg ccgggcgccg ccggggccgc gggccggatg cgcgtgcgca
      781 gatgctcggc gccgcgcccg gccagcatcg tctccgaaag caggccgaga cacgcctgct
      841 tcacctgaag gacgccctgg acaaggcgcc tgccctgcta cttgccggag gactcgcccc
      901 ggagccgcgc gcggcgtcgg ccaatgtaga ggaccagcac caccgctgcc acggccaaga
      961 ggatccactc cgtcatcggt cttcactcct tccgccggga acaccggctg cttgtcgcca
     1021 atcgcgcgcc ctagtcgggg aagttccgga ggaactgaat caccgccggg ccgagcacca
     1081 ccacgtacag cgcgggaaac aggaagaaca cgagggggaa caccagcttg acgcccacct
     1141 tcgcggccat ctcctcggcg gcctgccgcc gcttcagccg catgtcgtcg gccatggtcc
     1201 gcagggtctg cgccacgctc gtgccgaagc gatccgtctg gacgagcatc gcgaccaggg
     1261 ccctgacgtc ctccaccctg gtccgctggg cgaggttcct gaacgcctcg agccgggcca
     1321 tgccggcgcg cgtttccgtg ctgagaatgc gaagctcttc cgcgagcgcc ggataggcga
     1381 tgcccagttc ctcgctggcc ttgatgaccg cctggtcgag actgctgccg gcttccaggc
     1441 acacgatgag gaggtcgagc aggtccggca ggccgttgcg gatttgcttc cggcgggcac
     1501 cgatcatgga gtcgaggacg aacccgggcg cgatgtagcc gagggcgcct gcgaagagcg
     1561 ccgcgaccca gcccgcccgg tcgctcagtc ccaagaacga gaagacgccc acgacggcca
     1621 gcaccggcag cgcgatctcg gcgctcccga aggcgatggc cgcgagcggc gacttgatgc
     1681 cggcggcggc gaggcgttgc tccaggcccg acatgtcctg cgcggacttc ggcaggagcc
     1741 tggaccagcg ggcgagcttg ggatcccgct ctgacgtgag cggcccttgc agcaggacct
     1801 cggtcctggc ccgcgacggc aggatcgcgt cgaggcggcg ctgggcggcg gactgcttcg
     1861 acaggatcgc ggacgccgcc aggcccgcca gcagggccac ggacacgaac acggcgccga
     1921 gcgcgacaac gaattctacg ggcattgcgg ccctcgccct tcgtgcgtgc gcgccccggg
     1981 acggcgtccg ccgtctccag cctgcttgag ccggagcgag ggcccccagc cggcgcgagt
     2041 tccggggcta gtagtcgaca ttgacgatct tcctgatgac cagcgcgccg acgacctgga
     2101 agacgacggc tcccacgatc atctggatcc cgagccgttc gtcgagcatc gcggcgaagt
     2161 gtttcgggtt gatgatgctg aagatgacgg cgagcgccgg aggcagcccc accaggatcc
     2221 acccggtgac ccggccgtgg gcgctcctga cctgcacctg gcgcttgacc atgaagcggt
     2281 cgcggatgac ggaagcgagg ttggtgagga cctccgtgag attgcccccg gactcgcgct
     2341 gcgtcaggat ggccgtcacg aagaacttgg ccgcgagcag cgggacccgc tcgccgaacg
     2401 agcgcagggc ctcggggagc ggcatgccga agttctgctg gtcgtgcagc agcttgaatt
     2461 cggcggcgat cggctccggc agctcgtcgg ccaccatgcc gagggcggcg gcgaaggtgt
     2521 ggccggcgcg cagcgcgcgg gtcatgaggt cgagcgcctc gggaaacagc tcctcgaacc
     2581 ggcgcacgcg ctgcgcgccc ttgtaccgca ggaacagcac cggcgcggcg gccaggcacg
     2641 cgcccagcac gagcccgacc tgcagcgagc ccgaccaggt ctggccgacc agcaggccca
     2701 gcatgaagag gcatcccgcc gacagcagca cgacgccgac ggtcgtcttg aggcccgact
     2761 gatcgatcag ccggacgatc gggcccgtga ggttcatgcg gcgcgcgagc agctgctcga
     2821 gggccggcag gctgctcagg cgccgggcct cgctctgcac gctggacgag acgcgggcgc
     2881 ccgctttcgc cgctccgagg cgccggcgga gcgccagctc ggctgcccga gcggggcgct
     2941 cgatcagcgc ccaataggtc ccgagcacga tcacgacgac gagtgcgaac gtcagcgcgg
     3001 caaacgccac cacagccctc cggggtcagg tgatgttctt cacgtgctcg aacatgtccg
     3061 ccggcaggac gtggccggcg gtcgtcaggc gctcgctcat cttgggcctg atgccggtgg
     3121 cccggaacct gccgcagacc cttccctgct ggttgatccc caggcgctcg aagacgaaga
     3181 tgtcctgcat cgagatcacc tcgccttcca tgccggtgag ctcggtgacc gatgtcacct
     3241 tgcgggtgcc gtcggccatg cggctcatgt gcacgacgag gttcacggcc gaggccacct
     3301 gctggcggat cgcccgctcg gggatgttca ggttggccat ggcgaccatg gtgtcgaggc
     3361 ggtacagcgc gtcgcgggcg ctgttggcgt ggatggtggt caggctgccg tcgtggccgg
     3421 tgttcatggc ctgcagcatg tccagcgctt catcgccgcg cacctcgccc acgatgatgc
     3481 ggtccggccg catgcggagg gcgttgatca ccagttgccg ctgccggatc gcgcctttgc
     3541 cctcgatgtt gggcggccgc gtctcgaggc gcaccacgtg gcgctgccgc aggacgagct
     3601 cggcggcgtc ttcgacggtg acgatacgct ccctgtccga gatgaagccg gagagcacgt
     3661 tgagcagcgt cgtcttcccc gcgcccgtgc cgcccgagac gatgacgttg gtccggcagg
     3721 cgacggcggc gcggatgaag tccagcatcg gctgggtcag cgagtcgcgc tccacgaggt
     3781 cctgggcgcc gagccgatcg gtgcggaacc gccggatcga cagcgcgggg ccgtccagcg
     3841 ccagcggcgg gatgatggcg ttcacgcggg agccgtccgc gagcctcgcg tccaccatgg
     3901 ggctcgattc gtcgatgcgg cggcccaccg agctgacaat gcgctcgatg atccgcatca
     3961 ggtgctggtc gtccttgaac accacgtcga ccggctccag cacgccgtgg cgctcgacgt
     4021 acacctggtc gaaccggttc acgaggatgt ccgacacctc ctggtccgcg aacagctcct
     4081 cgagcggacc caggccgaac agctcgttga ggacgtcgcc gatgagcgac tgccgctcga
     4141 agaggctgag ggggacctga tcgctctcgg cgtccagcat ccccatgatg acgccgcgga
     4201 tctccggctc ggcttcgttc cggctgaccc tggtgagccg ctccagattg aggcggctca
     4261 gcagcccctg gtgcacccgg cttttcagct cctggtactg cgggtggcgc gtatcaacgc
     4321 ggggcaccgc gggtgggaca ggtgtcatgg tctgcctcag tcggcgctca cgagcggccc
     4381 gtcaggcggc cgaagatgct ccggccgccc ctctgcggct cctcggccgg caggtccgcc
     4441 agttcgcgcg caagctcctt gagcgccccg gcgaggcctg tctgatcgct cagcgcgagc
     4501 ggccgtcccg tattgagggc ttcaagcgcc acgcggtagt cggacggcag gaggtggcgg
     4561 atcttcgagc cgaccacgcg ctcgatgtcc tcctggccga tgccggcgtt cggatcgtac
     4621 cggctgatgg ccagcacgac gcggtccttg ccgtagcgcc tgttcagggt ctcggccatc
     4681 cggctcgcgc cccgcacggt ggccagctcc tggttggcga tcaccaggat cttcttcacc
     4741 gggtcgaggc cgtccagcac ggcagggtcc gtgcggcaca ggtccagcac cacgtagcgg
     4801 tagtggcggg cggcggagtc gacgagcgcc cgcacccggc cggcgtccac cagcaggccc
     4861 agggcgcgat cgggcgaggc cagcagctcc gggcccgcct tcgtcttgac gcagaggctc
     4921 ttcaggaacg cggcgtcgag ccggtgcgtg ttctccagcg catcggcgac cgagaacctc
     4981 ggctcggcgg ccaggtagat ggcggcatcg ccgtacgcca ggtgcaggtc caccagcagc
     5041 acgctgcccg cccgcaggcg ggacagctcg gtggccacgt tgacggcggc cgtcgtcgtg
     5101 cccacgccgc ccttcgcgcc gaggaacgcg aacacgtcac ccgccggccc ggcgtcccgg
     5161 gacgcggcga tgcggccgag cgcggcgtcg agatcgtccc ccgcgagggg atcctgcagg
     5221 cactcgctga cgccggcccg catcgcctcg agcatgaggg ccgggtcgag cgccgacacc
     5281 agcagcagaa tcgggatcgc cgggtgctgc cggcggaacg cgtccaggcc ggcggggagc
     5341 gtccgctgcg gctccccccg caagtcgagc atgacgacgt cggccaccgc cgacgcctgc
     5401 tgcgctttgg ccgagaggtc ccgctcgctc cacgtcagga cggtcaggcc cctcgcgcgc
     5461 agcagttgct cgacttgccg gtccccggct ccgacaaacg cgatgtgccg ctgcaacgct
     5521 cttctactcc ctcgg
//
LOCUS       DAOSIF010000163         5531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13194_length_5531_cov_4.235391, whole genome shotgun sequence.
ACCESSION   DAOSIF010000163 DAOSIF010000000
VERSION     DAOSIF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13194_length_5531_cov_4.235391"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLT35_06820"
     CDS             <1..373
                     /locus_tag="PLT35_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG28978.1"
                     /translation="VIGVTPAEVEDVVTYYTMFRSKPAGTYLLQVCRTLSCALNGAER
                     VTDALARKLGIAPGETDATGMFTLVEVECLGACDRGPVVMVNDGWHECVTPEGAERLV
                     DELREKGTAALSGCHLRVEKP"
     gene            370..1653
                     /gene="nuoF"
                     /locus_tag="PLT35_06825"
     CDS             370..1653
                     /gene="nuoF"
                     /locus_tag="PLT35_06825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOG28979.1"
                     /translation="MTEPVLTAFVREPGGFTLEASIRHGAYEGLRKALAMEPGRVIEA
                     VKASGLRGRGGAGFPTGMKWGFVPKDSPGPKYVCCNADESEPGTFKDHVLMERNPHLV
                     LEGVAIACYAIGARTGYIYIRGEFLHVAEVLESAIAEARAAGCLGRGILGSAFDCEIH
                     VHRGAGAYEAGEESALLESLEGRRAQPRLRPPFPAVVGLYGCPTVINNVETLANVPLI
                     LAKGPEWFAAIGPEQNTGPKLFCVSGHVARPGVYEASMHTTLRELIYGYAGGIRGGRA
                     LKAVIPGGSSTPVLPPGAIDAPASFDGMIKAGSMLGSAAVIVMDDTTCMVWAAENLVH
                     FYRHESCGKCTPCREGSDWMLKILQKIERGEGEMRDLGLLESVARNIAGKTLCPFGDA
                     EVAPVLGTLEHFRHEYEAHVREGRCPFHAGWRRGR"
     gene            1650..2675
                     /gene="nuoH"
                     /locus_tag="PLT35_06830"
     CDS             1650..2675
                     /gene="nuoH"
                     /locus_tag="PLT35_06830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOG28980.1"
                     /translation="MIPLYVAQIGVVVAVFVMLLLAAAVMVYSERKVAGAIQQRYGPY
                     LVGPKGALQPFADVIKLILKEPLRPVAADRVLYYVAPLLSATAAFAAFAVVPFGAETT
                     LFGLIEEPIRLQVADVNVAVLVIFAITSISVYGIVLAGWSSNSKYSLLGALRSASQMI
                     SYELSYGVSLASVLIIAGSLSLTEIVDAQQGAWLGVLPRWNVFVQPVGFLVFMIAGVA
                     ETNRAPFDLPEAEQELVAGYHTEYSSTPFMMFFLAEYINMVTAAAVAANLFLGGWHGP
                     FLPESLGWIWFLAKTGAILFFYIWMRWTLPRLRYDQLMQFGWKVLLPVAALNLILTAA
                     GVITVGR"
     gene            2665..3195
                     /locus_tag="PLT35_06835"
     CDS             2665..3195
                     /locus_tag="PLT35_06835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOG28981.1"
                     /translation="MGAEMLLFLLAAAVAVAASLAVVLGHNPMHSVLLLIVSFAALAA
                     LYIGLDAPFAAVVQIIIYAGAIMVLFLFVVMLLNAHREDEGAGGAALGGRPLAAGAAL
                     AIVLAAELAWAVLRTRRAGGSWMGAGPESDGAALQSVRTLGGVLYSEYSLAFEVTSLL
                     IIVAMVGAVVLARRES"
     gene            3200..3502
                     /gene="nuoK"
                     /locus_tag="PLT35_06840"
     CDS             3200..3502
                     /gene="nuoK"
                     /locus_tag="PLT35_06840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOG28982.1"
                     /translation="MVPLGHYLFLSAVLFAVGTVGVFLRRNVITVLLSIEVMLNAVNL
                     AFIAFGRFQGSVHGQVIMFFVVTVAAAEAAVGLALAIALFRHRESLNPDAFTSLKW"
     gene            3511..>5531
                     /gene="nuoL"
                     /locus_tag="PLT35_06845"
     CDS             3511..>5531
                     /gene="nuoL"
                     /locus_tag="PLT35_06845"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOG28983.1"
                     /translation="MLLLIPLLPLLGFLVNAAIGRRLARAASAGVALAAMLGSLLASA
                     WAVLALAGLPEGSRAIVQQVAPWIESGALAAPFTLRLDPLAAVMVLVVTGVGFLIHVY
                     STAYMHDERPSEYARYFSYLNLFAAFMLVLVLGANFLVMFVGWEGVGLCSYLLIGFWF
                     TRKSASDAGKKAFIVNRIGDFGFLLGVFLIWVAFGTLDFQEVAGLAAQAPPEAAFGTV
                     SLAALLLFAGATGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMIGRNAVLF
                     AHAPIALGVVAAVGVATALMAGTIGLVQNDIKRVLAYSTVSQLGLMFVAMGVGAFAAG
                     IFHLYTHAFFKALLFLASGAVIHALAGEQDIRKMGGLKGELPVTYWTFLVGALAISGV
                     PLLSGFFSKDEILWKAYAGGHTLVWAVAVVASLLTAIYMFRLVFLTFHGSRASASRGL
                     AEAGHDRASGGGLPHAGGGGRLHDAPPAMAVVLVLLAVGSAVAGYIGVPAALGGSNRI
                     EHFLAPSFAAPGVAASGEHGANLAEARGEMVPGSGPQGEGGLARAGEGGHGSDDHAAE
                     LGLMALSSGVALAGIALAAYFFLRRREAADAVAARFAGLHRLLLNKYYVDEIYDAAIV
                     QPIRRGSERALWKAVDEGVVDGAVNGAGTIVSGAGAALRRLQTGSMRAYA"
BASE COUNT          687 a         1966 c         1955 g          923 t
ORIGIN      
        1 ggtcatcggc gtcacgccgg ccgaggtgga ggacgtcgtg acctactaca cgatgttccg
       61 ctcgaagccg gcgggcacgt acctgctgca ggtctgccgg acgctgtcgt gcgcgctgaa
      121 cggcgccgag cgcgtcaccg acgcccttgc ccgcaagctc ggcatcgcgc cgggcgagac
      181 cgacgccacg ggcatgttca ccctcgtcga ggtcgagtgc ctgggggcgt gcgaccgcgg
      241 accggtcgtg atggtgaacg acggctggca cgagtgcgtg accccggagg gcgccgagcg
      301 cctggtggac gagttgagag agaaagggac cgccgcgctg agcggctgtc acctgcgcgt
      361 ggagaagccg tgacggagcc ggtgctgacc gcattcgtcc gcgagccggg cgggttcacg
      421 ctcgaggcct cgatccgcca tggcgcctac gaagggctgc gcaaggcgct cgcgatggag
      481 cccggccggg tcatcgaggc cgtgaaggcg tcgggcctgc ggggccgcgg cggcgcgggc
      541 ttcccgacgg ggatgaagtg ggggttcgtc ccgaaggact ccccgggccc gaaatacgtc
      601 tgctgcaacg ccgacgagag cgagccgggg accttcaagg accacgtgct gatggagcgc
      661 aacccccatc tcgtgctcga gggcgtggcc atcgcctgct acgccatcgg cgccaggacg
      721 ggctacatct acatccgggg cgagttcctg cacgtggccg aggtgctcga atcggccata
      781 gccgaggcgc gcgcggcggg ctgcctgggc cggggcatcc tcgggagcgc cttcgactgc
      841 gagatccacg tgcaccgggg cgccggcgcg tacgaggccg gcgaggagtc ggcgctgctc
      901 gagtcgctcg aaggcaggcg ggcccagccg cggctgcgcc caccgttccc ggcggtggtc
      961 ggcctctacg gctgcccgac cgtcatcaac aacgtcgaga ccctcgccaa cgtgccgctc
     1021 atcctcgcga agggccccga gtggttcgcc gccatcgggc ccgagcagaa caccgggccg
     1081 aagctcttct gcgtgagcgg gcacgtggcg cggccgggcg tgtacgaggc gtcgatgcac
     1141 acgacgctgc gcgagctcat ctacggctac gccggcggga tccgcggcgg acgggccctc
     1201 aaggccgtga tcccgggcgg ctcctcgacg ccggtcctgc ccccaggcgc gatcgacgcc
     1261 ccggcgagct tcgatggcat gatcaaggcc gggtcgatgc tcgggtcggc ggccgtgatc
     1321 gtcatggacg acaccacgtg catggtgtgg gccgccgaga acctggtcca tttctaccgc
     1381 cacgagtcct gcggcaagtg cacgccgtgc cgcgagggct cggactggat gctgaagatc
     1441 ctgcagaaga tcgagcgggg cgagggggag atgcgcgacc tcggcctgct cgaaagcgtg
     1501 gcgcgcaaca tcgcgggcaa gacgctgtgc ccgttcggcg acgccgaagt ggcgccggtg
     1561 ctgggcaccc tcgagcactt ccgccacgag tacgaggcgc acgtccggga ggggcgctgc
     1621 ccgttccacg ccggctggag gcgggggcga tgatcccgct gtacgtggcc cagatcggcg
     1681 tcgtcgtcgc cgtcttcgtc atgctgctcc tggcggcggc cgtcatggtg tactccgagc
     1741 gcaaggtcgc cggggccatc cagcagcgct acgggcccta cctcgtcggc ccgaagggcg
     1801 cgctccagcc gttcgccgac gtcatcaagc tcatcctcaa ggagccgctc cggcccgtcg
     1861 ccgccgaccg cgtcctctac tacgtggcgc cgctcctctc ggcgacggcc gccttcgcgg
     1921 ccttcgccgt cgtgcccttc ggcgccgaga cgacgctgtt cgggctgatc gaggagccga
     1981 tccgcctgca ggtcgccgac gtgaacgtgg ccgtgctcgt gatcttcgcc atcacgtcga
     2041 tcagcgtcta cggcatcgtc ctcgccggct ggagctcgaa cagcaagtac tcgctgctcg
     2101 gcgcgctccg ctcggcgtcg cagatgatca gctacgagct gtcttacggc gtctcgctgg
     2161 cgtcggtcct gatcatcgcg ggctcgctct cgctcaccga gatcgtggac gcgcagcagg
     2221 gcgcgtggct cggggtcctc ccgcgctgga acgtcttcgt ccagcccgtc ggcttcctcg
     2281 tcttcatgat cgccggcgtg gccgagacca accgcgcgcc cttcgacctc cccgaggccg
     2341 agcaggagct ggtggcgggc taccacaccg agtacagcag cacgccgttc atgatgttct
     2401 tcctcgcgga gtacatcaac atggtgaccg cggccgccgt ggccgcgaac ctcttcctcg
     2461 gcggctggca cgggccgttc ctccccgagt ccctcggctg gatctggttc ctcgccaaga
     2521 ccggcgccat cctcttcttc tacatctgga tgcggtggac gctgccgcgc ctgcgctacg
     2581 accagctcat gcagttcggg tggaaggtgc tgctcccggt cgcggcgctc aacctgatcc
     2641 tcaccgcggc gggggtgatt accgttgggc gctgagatgc tgctgttcct gctcgccgcc
     2701 gccgtcgccg tcgcggcctc cctcgcggtg gtgctgggcc acaacccgat gcacagcgtg
     2761 ctgctgctca tcgtgtcgtt cgccgccctc gccgcgctct acatcgggct ggacgcgccc
     2821 ttcgccgccg tcgtgcagat catcatctac gccggcgcga tcatggtgct gttcctcttc
     2881 gtggtgatgc tcctgaacgc gcaccgggaa gacgaggggg caggcggcgc ggcgctcgga
     2941 ggcaggcccc tcgcggccgg cgccgcgctg gccatcgtgc tcgcggccga gctggcctgg
     3001 gccgtcctgc gcacgcgccg cgcgggcggg tcgtggatgg gcgccggccc ggagtccgac
     3061 ggggccgccc tccagtcggt gcgcaccctt ggcggcgtgc tctactcgga gtactccctc
     3121 gccttcgagg tgacgtcgct gctcatcatc gtggccatgg tcggggccgt cgtcctggcc
     3181 aggcgggagt cgtagcgcga tggtgccgct gggccactac ctcttcctct ccgccgtgct
     3241 cttcgccgtg ggcaccgtcg gcgtgttcct gcgccgcaac gtcatcacgg tgctcctgtc
     3301 gatcgaggtg atgctgaacg ccgtcaacct cgcgttcatt gccttcggcc gcttccaggg
     3361 atccgtccac ggacaggtga tcatgttctt cgtcgtcacc gtcgcggcgg ccgaggcggc
     3421 cgtgggcctg gcgctcgcca tcgccctgtt ccggcaccgg gagtcgctca accccgacgc
     3481 cttcacgagc ctgaagtggt gaggggaccc gtgctgctcc tcattccgct gctgccgctc
     3541 ctcgggttcc tggtcaacgc cgcgatcggg aggcgcctgg cccgggccgc ctccgccggc
     3601 gtggcgctgg cggcgatgct cggatcgctg ctcgcgtcgg cctgggccgt cctggcgctc
     3661 gcgggcctgc ccgaaggctc gcgggcgatc gtgcagcagg tggcgccctg gatcgagtcc
     3721 ggcgcgctcg cggcgccgtt cacgctcagg ctcgacccgc tcgccgcggt gatggtcctg
     3781 gtcgtcacgg gcgtcgggtt cctgatccac gtgtactcga cggcctacat gcacgacgag
     3841 cggccgagcg agtacgcgag gtacttctcc tacctgaacc tcttcgcggc cttcatgctc
     3901 gtgctcgtcc tcggcgcgaa cttcctcgtg atgttcgtgg gctgggaggg cgtggggctc
     3961 tgctcgtacc tcctcatcgg gttctggttc accaggaagt cggcctccga cgccggcaag
     4021 aaggccttca tcgtcaaccg catcggcgac ttcggcttcc tgctgggcgt cttcctgatc
     4081 tgggtggcct tcggcaccct cgacttccag gaggtcgccg gcctggcggc ccaggcgccc
     4141 ccggaagccg cgttcgggac cgtctcgctg gcggcgctgc tgctgtttgc cggcgcgacg
     4201 ggcaagtccg cccagatccc gctctacgtg tggctgccgg acgcgatgga gggcccgacg
     4261 cccgtctccg ccctcatcca cgccgcgacg atggtgacgg ccggcgtgta catgatcggg
     4321 cgcaacgcgg tgctgttcgc ccacgcgccg atcgcgctgg gcgtcgtggc ggccgtcggg
     4381 gtggcgaccg cgctcatggc cggcacgatc ggcctcgtgc agaacgacat caagcgcgtc
     4441 ctcgcctact ccaccgtgtc gcagctcggg ctgatgttcg tcgcgatggg cgtcggcgcc
     4501 ttcgccgcgg ggatcttcca cctctacacg cacgcgttct tcaaggcgct gctcttcctc
     4561 gcgtccggcg cggtcatcca cgccttggcg ggggagcagg acatccggaa gatgggcggc
     4621 ctgaaggggg agctgccggt cacgtactgg acgttcctcg tcggcgcgct cgcgatctcg
     4681 ggcgtgccgc tgctctccgg cttcttcagc aaggacgaga tcctctggaa ggcgtatgcc
     4741 ggcggccaca cgctggtgtg ggccgtggcc gtcgtcgcgt cgctgctgac ggcgatctac
     4801 atgttccgcc tcgtcttcct cacgttccac ggctcgcgcg cgagcgcgag ccgcggtctc
     4861 gccgaagccg ggcatgatcg ggccagcgga ggcggactcc cgcatgcggg cggaggcggg
     4921 cgcctccacg acgcgccgcc cgcgatggcc gtcgtgctcg tgctgctcgc cgtcgggtcc
     4981 gccgtggcgg gatacatcgg cgttcccgcc gccctcggcg gctccaaccg catcgagcat
     5041 ttcctcgcgc cgagtttcgc cgctccgggc gttgcggcga gcggcgaaca cggcgcgaat
     5101 ctcgccgaag cccgaggcga gatggttccc gggagcgggc cgcagggcga aggcggactc
     5161 gcgcgcgccg gcgaaggcgg ccatggctcc gacgaccatg ccgccgagct cggcctcatg
     5221 gccctgtcga gcggcgtcgc gctggccggc atcgccctcg cggcgtactt cttcctgcgc
     5281 cggcgcgaag cggccgacgc ggtggccgcc cgcttcgcgg gcctccaccg gctgctcctc
     5341 aacaagtact acgtggacga gatctacgac gcggccatcg tgcagcccat ccggcgcgga
     5401 tccgagcgcg cgctgtggaa ggcggtggac gagggcgtcg ttgacggcgc cgtcaacggc
     5461 gccgggacga tcgtcagcgg cgccggcgcg gcgctgcggc ggctgcagac cggctcgatg
     5521 cgcgcgtacg c
//
LOCUS       DAOSIF010000164         5523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13223_length_5523_cov_3.898866, whole genome shotgun sequence.
ACCESSION   DAOSIF010000164 DAOSIF010000000
VERSION     DAOSIF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13223_length_5523_cov_3.898866"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLT35_06850"
     CDS             complement(<1..728)
                     /locus_tag="PLT35_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG28984.1"
                     /translation="MARPGVLVTCRIPTSVVERLEAACDVEVFAGPDAMPPGELKARL
                     EGKQALLCLLTDRITADVLDAGRDLRIVANIAVGFDNIDLDAARARGVVVSNTPDVLT
                     QATADLTWGLILAATRRIPEGERLLRRGGWSGWALDFLLGSDLRDKQLGIVGLGRIGA
                     AVASRAAAFGMRVAYATLDEAAAGPSAADPAWARLGLDELLSSSDVVSLHVPLTPSTR
                     HLIDRRAILRMKRRAYLINTSRGPV"
     gene            complement(733..996)
                     /locus_tag="PLT35_06855"
     CDS             complement(733..996)
                     /locus_tag="PLT35_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28985.1"
                     /translation="MLARILVWAFFLLVIWRAIRSLISGIVQGASAPPPPGGRIPPDA
                     GERMVRDPVCGTFVLPSRSLFLRDRTGVHHFCSAVCREKYRAR"
     gene            complement(1009..1668)
                     /locus_tag="PLT35_06860"
     CDS             complement(1009..1668)
                     /locus_tag="PLT35_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG28986.1"
                     /translation="MRAESIVAGVAGAVFGLLVGWIIGTQQGSARPAGAAPAPFAQAA
                     PAGSMPPAVAAVDEARAAALKGIAAGDAANVQSRVQLGNLYFDAERYQEAIRWYDEAL
                     RLNPKDVNVSTDLAVCFYYTNQPDRAISQLEESLRIDPAHTKSLLNLGIVKSFGKQDL
                     AGAMAAWEEVIKVAPGSEEGQSARRLLDNLRSAHPGGTAMPPPGTPAATETPKPAGSG
                     R"
     gene            complement(1676..2386)
                     /locus_tag="PLT35_06865"
     CDS             complement(1676..2386)
                     /locus_tag="PLT35_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOG28987.1"
                     /translation="MPAAVIVTACVALLGPAAPARAQGPQPVTFAARDGVRVSGVLYV
                     PAQQPAPGVVLLPMLGRTHRDWEAAAATFAESGLAALAIDFRHAAGGAVEAPDPGFSA
                     LVLDADAARAYLAARPEVDPARIGMAGGSLGANVAALSAGNDPSVRSLALLSVSGDYR
                     GLKMEQALRSFGARPALLVASSEDWYALRSARQAVSMGGGTRELRVLAGMGHGLVMLA
                     RDPDLGLVLVDWFKRTLL"
     gene            complement(2539..3249)
                     /locus_tag="PLT35_06870"
     CDS             complement(2539..3249)
                     /locus_tag="PLT35_06870"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOG28988.1"
                     /translation="MRLSLNIDHIATIREARRAREPEPVTAAVIAELAGAEGITVHLR
                     GDRRHIQDRDVELLRRIVATKLNIEMAATPEMIEIARRIKPDQVTLVPERPDEVTTTG
                     GLDVLQHGDAVRHAVAACGAAGIRVSVFVDAIGAQVDASKAAGADAIEINTGPYADAP
                     GQQRAAELARIALAADRAAERGLEVLAGHGLNYVNVAPIVKLSPIVEANIGHSIIARA
                     ALVGLDRAVREMVALLKS"
     gene            complement(3444..>5523)
                     /locus_tag="PLT35_06875"
     CDS             complement(3444..>5523)
                     /locus_tag="PLT35_06875"
                     /inference="COORDINATES: protein motif:HMM:NF006194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HOG28989.1"
                     /translation="DAARCVDQYAEAWGEPLALRTYTSRLLGADPALVLHGGGNTSVK
                     APWRTVLGEDAAAVFVKASGLALAGIEPAGHPALDLGALQRLRALEALAEDAMVNELR
                     RARFDAGAPVPSVETLLHAFLPGAFIDHTHADAVLALTNQRDGAALAQEAFGSAAALV
                     PYAAPGFDLARSAAEAAERGAPALVLMKHGLVTWAPTAREAYERHIDLVARAEAFAAA
                     RGRRGTVVTVPAGAFGEAVRRFEAAAPVLRGRLAHATGDADRPWDRVLLQPLITEPVL
                     ALLATPGARDLLVTPPLTPDYLIRTKALPLWIGDPAWDDLARFSAQCGEAIRAYSAEY
                     EAYVGRHRADLEDGLAAFDPRPRVVLVPGLGAVCAGPTARDAAMARDITAMTLQVKAD
                     VARMGAYEGLGERELFLQEYRGVQRAKLRAPDGPLADRVAVVTGAAGAIGAGIAEGLL
                     RAGCHVVLTDLPGAPLDSLAAELDARFPGRAVGAPMDVTEQASVAEGFRFAARTWGGV
                     DLVIVNAGVAHVSALSAMDLEAFRRVERVNVEGALLVVSESGRHFALQGTGGDIVLVS
                     TKNVFAPGAKFGAYSATKAAAHQLARIASLELADIDVRVNMVAPDAVFSHGPRRSGLW
                     AAVGPDRMKARGLDEAGLEEYYRSRNLLKARVTARHVANAVLYFATRQSPTTGATIPV
                     DGGLPDATPR"
BASE COUNT          739 a         2077 c         1990 g          717 t
ORIGIN      
        1 accgggccgc gcgacgtgtt gatcaggtac gcgcgccgtt tcatgcggag gatcgcgcgg
       61 cggtctatca ggtggcgggt cgacggcgtc agcggcacat gaagcgagac cacgtcggag
      121 gaagacagga gctcgtccaa tccgaggcgc gcccatgccg gatcggccgc cgacgggccg
      181 gccgccgcct cgtcgagcgt cgcgtaggcg acgcgcatgc cgaacgccgc cgcccgggac
      241 gcgacggccg cgccgatccg cccgagcccg acgatgccga gctgcttgtc gcggaggtcg
      301 gacccgagca ggaagtccag cgcccagccg gaccagccgc cgcgcctcag cagccgctcg
      361 ccctccggga tgcgccgcgt ggccgccagg atcaggcccc acgtcaggtc cgccgtggcc
      421 tgcgtgagca cgtccggcgt gttcgacacg acgacgcccc gcgcccgggc ggcgtcgaga
      481 tcgatgttgt cgaatccaac cgcgatgttc gcgacgatgc gcaggtcgcg gcctgcgtcg
      541 agcacgtcgg cggtgatccg gtcggtcagg aggcagagca gcgcctgctt gccctccagc
      601 ctcgccttga gctcgccggg cggcatcgcg tccggcccgg cgaacacctc cacgtcgcac
      661 gcggcctcga gccgctcgac gaccgacgtc gggatgcggc acgtgacgag aacgccgggc
      721 ctggccatga cgtcagcgcg cgcggtactt ctcgcggcag acagccgagc agaagtggtg
      781 gacgccggtc ctgtcgcgca ggaagagcga gcgcgaggga agcacgaacg tgccgcacac
      841 cgggtcgcgc accatccgct cgcccgcgtc cggagggatg cgcccgccgg gaggcggtgg
      901 agcggaggcg ccctgcacga tgcccgagat caggctgcgg atcgcgcgcc agatgacgag
      961 caggaagaac gcccagacca ggatgcgagc cagcatctgc gggctcctct agcggccgga
     1021 gccggccggc ttgggggtct cggtcgccgc gggcgtgccg ggcggaggca tggcggtgcc
     1081 gccggggtgg gccgacctca ggttgtcgag caggcgcctg gcggactgcc cctcttcgct
     1141 gccgggcgcg accttgatga cctcttccca cgccgccatg gccccggcga ggtcctgttt
     1201 gccgaaggac ttgacgatcc cgaggttcag cagcgacttc gtgtgggccg gatcgatgcg
     1261 cagcgactcc tcgagctggc tgatcgcgcg gtcgggctga ttcgtgtagt agaagcagac
     1321 cgccagatcc gtgctgacgt tcacgtcctt cgggttcagc cggagcgcct cgtcgtacca
     1381 gcggatggcc tcttggtagc gctcggcgtc gaagtagagg ttgccgagct ggacgcgcga
     1441 ctggacgttg gcggcgtcgc ccgccgcgat gcccttcagc gccgccgccc gcgcctcgtc
     1501 cacggcggcg acggccggcg gcatgctccc cgcaggggcc gcctgggcga agggggctgg
     1561 cgccgcgccc gccggccggg cgctcccctg ctgcgtgccg atgatccagc cgaccagcag
     1621 gccgaacacg gcgcccgcga cgccggcgac gatggattcc gccctcatgg gccgatcata
     1681 gcaacgtgcg cttgaaccag tccaccagca cgagcccgag gtccggatcc ctggcgagca
     1741 tcacgaggcc gtgtcccatg cccgccagca cccgcagctc ccgggtgccg ccgcccatgc
     1801 tgaccgcctg gcgcgcggat cgtagcgcgt accagtcctc gctgctcgcg acgaggagcg
     1861 cggggcgcgc cccgaagctc cggagcgcct gctccatctt caggccgcgg tagtcgccgc
     1921 tgaccgacag cagcgcgagg gatcgcacgg acgggtcgtt gccggccgag agcgcggcca
     1981 cgttcgcccc gagcgaccct cccgccatgc cgatcctcgc ggggtcgact tccggccgcg
     2041 cggccaggta cgcgcgcgcg gcgtccgcgt cgagcaccag cgccgagaag ccggggtccg
     2101 gcgcctccac cgccccgcca gccgcgtgcc ggaagtcgat ggcgagcgcg gcgaggcccg
     2161 actcggcaaa cgtcgcggcc gccgcctccc agtcgcggtg cgtccggccg agcatcggca
     2221 gcagcacgac tccgggcgcc ggctgctgcg ccggcacgta cagcacgccg gacacccgca
     2281 cgccgtcccg cgccgcgaac gtcaccggct gagggccctg cgcccgggcg ggcgcagcgg
     2341 ggccgagcag tgcgacacac gcggtgacga tgacagccgc gggcatgagc gccagcccgg
     2401 cccgcggccc ccagctccag gcctgcgcgt ccaccagacg gcgtgccggt cggcttcggc
     2461 gcggcaggcc tggcgccgag tcccgagtcc cgagtcccga gtcccgagtc ccggatgccg
     2521 catcccggat gtcggctgtc atgacttgag cagcgccacc atctcgcgca cggcgcggtc
     2581 gagccccacg agcgcggcgc gcgcgatgat gctgtgcccg atgttcgcct cgacgatggg
     2641 gctcagcttc acaatcggcg cgacgttgac gtagttcagg ccgtggcccg ccaggacctc
     2701 gaggccccgc tcggcggccc ggtcggcggc cagcgcgatg cgggcgagct ccgccgcccg
     2761 ctgctggccc ggcgcgtcgg catacgggcc ggtgttgatc tcgatggcgt cggcgccggc
     2821 ggccttcgac gcgtccacct gcgcgccgat agcgtctacg aacacgctga cccggattcc
     2881 cgcggccccg cacgccgcca ccgcgtgtct gacggcgtcg ccgtgctgca gcacgtccag
     2941 ccctcccgtc gtggtcacct cgtcggggcg ctcggggacc agcgtcacct ggtccggctt
     3001 gatgcggcgc gcgatctcga tcatctccgg cgtcgcggcc atttcgatgt tcagcttcgt
     3061 ggcgacgatc cggcgcagca gctcgacgtc gcgatcctgg atgtgccggc ggtcgccgcg
     3121 caggtgcacg gtgatgcctt ccgctccggc cagctcggcg atgaccgcgg ccgtgacggg
     3181 ctccggctcc cgggccctgc gcgcctcgcg gatggtggcg atgtgatcga tgttgaggct
     3241 caaacgcata gcagctacct catcgataac cagaagtcag catccagaag ccggaatccg
     3301 gaatgaggca tcgagacccc ctggcttcat gccgacgccc acgcgcgacc ggacgccgcc
     3361 cgctcgccgc agtgctcttg ccttccctat tctgcattct ggatctgaat tccggattcc
     3421 ggattccctc tcccattccc gcttcaccgt ggcgtcgcgt ccggcagccc gccgtcgacc
     3481 gggatggtcg caccggtcgt gggcgactgg cgcgtcgcga agtacagcac ggcgttcgcc
     3541 acgtggcgcg cggtgaccct cgccttgagc aggttgcgcg agcggtagta ctcctccagg
     3601 ccggcttcgt ccaggccgcg cgccttcatg cggtccggcc cgaccgccgc ccagagtccc
     3661 gaccgccgcg ggccgtgcga gaacacggcg tctggcgcca ccatgttcac cctgacgtcg
     3721 atgtccgcca gctcgaggct ggcgatgcgc gccagctggt gcgcggccgc cttcgtcgcg
     3781 ctgtaggcgc cgaacttcgc gcctggcgcg aagacgttct tggtggacac cagcacgata
     3841 tcgccgcccg tgccctggag ggcgaagtgc cgtccgctct ccgagacgac gagcagcgcg
     3901 ccctcgacgt tcacgcgctc gacccggcgg aacgcctcga ggtccatcgc cgaaagggcc
     3961 gacacgtgcg cgacccccgc gttgacgatg accaggtcca cgccgcccca ggtgcgcgcg
     4021 gcgaaccgga agccctcggc caccgacgcc tgctcggtga cgtccatcgg cgcgcccacc
     4081 gcccggccgg gaaacctggc gtcgagctcc gcggcgagcg agtcgagggg cgccccgggg
     4141 agatccgtca gcacgacgtg gcagccggcc cgcagcagcc cctcggcgat ccccgctccg
     4201 atggcgcctg cggcgcccgt cacgacggcc acccggtcgg ccagggggcc gtcgggtgcg
     4261 cgcagtttcg cgcgctggac gccgcggtat tcctgcagga acagctcgcg ctcgccaagc
     4321 ccttcgtagg cccccattcg cgccacgtcc gccttgacct gcagcgtcat cgcggtgatg
     4381 tcgcgcgcca tggcggcgtc gcgcgccgtc gggccggcgc agactgcgcc gaggcccggg
     4441 acgagcacga cgcgcggacg cgggtcgaac gccgcgaggc cgtcctccaa gtccgcgcgg
     4501 tggcggccga cgtaggcctc gtactccgcg ctgtaggccc ggatggcctc gccgcactga
     4561 gcggagaagc gcgcgaggtc gtcccacgcg gggtccccga tccagagggg gagcgccttc
     4621 gtcctgatca ggtagtccgg cgtcagcggc ggggtgacga gcagatcgcg cgcgccggga
     4681 gtcgccagga gcgccaggac gggctccgtg atgagaggct gcagcagcac gcggtcccag
     4741 gggcggtcgg cgtcgccggt ggcgtgcgcc agccggcccc ggagcacggg ggccgcggcc
     4801 tcgaagcgcc ggacggcctc gccgaaggcg ccggcgggca ccgtcacgac ggttccgcgc
     4861 cgcccgcgcg ccgccgcgaa ggcctcggcg cgcgccacca gatcgatgtg ccgctcgtac
     4921 gcctcgcgcg ccgtcggcgc ccacgtcacc aggccgtgct tcatcagcac cagcgccggc
     4981 gcgccgcgct cggcggcctc cgcagccgac ctggcgagat cgaagcccgg cgcggcgtac
     5041 ggcacgagcg cggcggcgct gccgaacgcc tcctgcgcga gggccgcgcc gtcgcgctgg
     5101 ttggtcaacg ccagcacggc atccgcgtgg gtgtggtcga tgaacgcccc cggcaggaag
     5161 gcgtgcagga gcgtctcgac cgacggcacc ggggcgcccg cgtcgaagcg ggcgcggcgg
     5221 agctcgttga ccatcgcgtc ttccgccagc gcctcgaggg cccgcaggcg ctggagggcg
     5281 ccgagatcca gggccgggtg cccagcaggc tcgatcccgg cgagggcgag gcccgaggcc
     5341 ttcacgaaga cggccgcggc gtcctcgccg agcaccgtgc gccacggggc cttcaccgac
     5401 gtgttgccgc ccccgtgcag gaccagcgcc gggtccgcgc ccagcagccg gctcgtgtag
     5461 gtgcgcagcg ccaggggctc gccccacgcc tcggcgtact gatcgacgca cctggcggcg
     5521 tcc
//
LOCUS       DAOSIF010000165         5469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13380_length_5469_cov_4.129664, whole genome shotgun sequence.
ACCESSION   DAOSIF010000165 DAOSIF010000000
VERSION     DAOSIF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13380_length_5469_cov_4.129664"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLT35_06880"
     CDS             complement(<1..902)
                     /locus_tag="PLT35_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG28990.1"
                     /translation="MNRKFETGHPIRVVVAAVALLAIMVAVGHHRVQALKYAKPEHSL
                     QPDPSLPVWVPGPLTIEPDEEFNVVGADVMDEMTLGWVKMMRKAYPRLSVTMEARASG
                     SGGPALTNGIAHAAPVGRELLPAEEKAFVDKFGYKPTAFRVATGSLGSLGKTAASIIM
                     VDGDNPITCLSLQQLDAIYSAERRRGGQPIRTWGDVGLKGEWADRPIHLYGLRHPNGI
                     EWYLRQEVLLGGPYRDDVEFVKGKGFTHAFNVAADDMRSRKGGMSYALLANIQPNTKA
                     IALSSEPGGKCVVPTTRTVYDHSYP"
     gene            1230..3638
                     /locus_tag="PLT35_06885"
     CDS             1230..3638
                     /locus_tag="PLT35_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG28991.1"
                     /translation="MRRIIGSRIPTVVLAILLVTTTAVFGQAPAGAKSSSPSGQTVAG
                     RVLDPSQSVIIGATVTLRSLAEDRTRDTQTDARGVYRFDQVAPGSYLLIAFRDGFTAI
                     TEELEVTPGVAANVDLTLHVAGFKEEVTVAFTALESRSALKMDTPLRDVPLTVNSYTG
                     SFMKTIESTNVGDLYSYTTGVTRSGNTGLDFVIRGVRASHTGNIQYNGLPGLAARVGS
                     PSTVNVERLEVLKGPSSVLYGQAQPGGLINIITKKPQAERQALLDIRSGGTFGGGMNG
                     RGKHHVSTDLTGPIDSGRKFLYRFIASYDDENSFRDNVTNKDLYLVPSLSWMGWAGAV
                     LNIEAEYRRTNTSLDSGLVAPFNDIKLVAPANVRYQEPSDYIEETGKTITTSLKKSWA
                     NGVTWTANWRSVWHEDLQVGFENAGTSGTQTVTRRDRRQRNLREYHYVDTTIGRSVKT
                     GSVNHKLLFGSTVGYEMTDYDRQQFATGAALNIDLYRPVFGAANLPLKPNTWRHSTAW
                     TGAGYLNDQIELSAMWKVLLGLRFEARRADEEELRVNPKTREKNSQAFLPLAGLVFQP
                     IDSLSLYGSYSTSFTPPPPGAIDKNGENPFKPESARQFEWGAKLQVWNGGELSVSWFD
                     ITKNDVLITLVAQAVNDQIGQERSKGLETTFNARLLNNWQVIVGYAYTDSEVSKDSDP
                     AKVGSRIPNSPRHGANFWTRYDVAKGALSGLGAGLGVVFTGERAGTISASSSTLPVLL
                     LPGYTRLDLGVYYVKKRYELTLRVVNLTDKKYYESNLGTTTTSLNIRPGAPRSGSMSL
                     RVRF"
     gene            3641..5128
                     /locus_tag="PLT35_06890"
     CDS             3641..5128
                     /locus_tag="PLT35_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG28992.1"
                     /translation="MANGAAKLAALAAGAALIISGSAAHAQEGALPPYRPVAVSCELN
                     SVGDGSMAPLMDALFTAYQKVQPGVRQGSRWDHQRAERAMPALMFELAEMAPLAREPR
                     PAELAPYAHQFAGDMMKAPLLVRIGRGADGGEPAVAVNKRPDSPLPPCVREFLAFALS
                     REGQALVAAGGRFGALTAAEAAREREKLQGYLPGLDPTLPVYAPEIAVSGEIASVGSD
                     GMKSLMERWMRDFQRVQPGVRKGERWEHLGTLNGFHALIADETMLAPMGRELWPEEAD
                     AYASTHGGRRLLEIRVARGGFNTPQRTTAQAIFVHQSNPLSRITLGQVAAIFGEPASI
                     TRWGQLGLSGEWKDRPITVYAPRLTAPNAMSVQLRVLNGGRWKASIREGTIAETAAAA
                     ASDQGSIAFGGFEEGGPGIKPLAVAESAEGPFYEGTSATVSGGQYPLARYMFIRLEAD
                     EKHLTPAVREFFRFILSRDGQEPVRYSGYFPLSNREVQEELRKLR"
BASE COUNT          932 a         1841 c         1842 g          854 t
ORIGIN      
        1 ggataggagt ggtcgtagac ggtccgcgtc gtcggcacca cgcacttgcc gccgggctcg
       61 ctcgacagcg cgatcgcctt ggtgttcggc tggatgttcg cgagcagggc atagctcatg
      121 ccgcccttgc gcgaccgcat gtcgtcggct gccacattga aggcgtgggt gaagcccttg
      181 cccttcacga actcgacatc gtcgcgatac gggccgccga ggagcacctc ctgcctgaga
      241 taccactcga tgccgttcgg gtgcctgagg ccgtacaggt ggatcggccg gtccgcccac
      301 tcgcccttca acccgacgtc gccccacgtg cggattggct gtccgcccct cctgcgctcg
      361 gcggaataga tcgcgtcaag ctgctgcagg ctcaggcagg tgatcgggtt gtctccgtca
      421 accatgatga tgctcgcggc ggtcttgccg agcgacccga ggctgcccgt cgcgacccgg
      481 aaggcggtcg gcttgtagcc gaacttgtcg acgaacgcct tctcctcggc aggcagcagc
      541 tcgcggccca cgggcgcggc gtgggcgatg ccgttcgtca gcgccgggcc gccggacccg
      601 gacgcgcgcg cctccatggt gacgctcagt ctgggatagg ccttgcgcat catcttgacc
      661 cagccgaggg tcatttcgtc catcacgtcg gcgccgacga cgttgaactc ctcgtcgggc
      721 tctatcgtga gcggccccgg cacccagacc ggaagcgacg gatccggctg gagagagtgt
      781 tccggctttg cgtacttgag cgcctggacc cggtgatgac cgacggcgac catgatggcc
      841 aacagtgcga cggctgccac caccacacgg atggggtggc cggtttcgaa cttccggttc
      901 atcctcgccc tcctctgtct ccggcgtgct cgggctccgg cactcccggc cgggagtgcc
      961 gcggcggcgc cgatcgcgcg gacctcgccg acggcgccac ctgcgcgggc agcatgttac
     1021 gcagccgtcg ggcacgctgc tacttaagtc cgcctgaagt ttccttcagg gtcccgcggc
     1081 gagcgaccgg ctcccgctcg tccgcggcgc cttaaccggg gttcagaaaa acttcagtat
     1141 ggtgcgcgcg gcaagcgatc tatacagggg cgcggtcgat tagtgcgttc cggatgctgg
     1201 atggagctgg atggagtgag gagggcatca tgcggcggat catcggttcg cggattccga
     1261 cagtcgtcct agcgatattg ctcgtcacga ccaccgctgt cttcggtcag gcacccgccg
     1321 gggcgaagtc gtccagtccg agcggccaga ccgtcgccgg gagggtcctc gacccttcgc
     1381 agtcggtgat tatcggcgcg acggtgacct tgcgcagcct ggccgaggat cgcacgcgtg
     1441 acacccagac cgacgcgcgc ggcgtctatc gctttgacca ggtcgcgccc ggctcgtacc
     1501 tgctgatcgc cttccgcgac gggttcaccg cgatcaccga ggagctggag gtgacgccgg
     1561 gcgtcgcggc gaacgtggat ctgacgctgc atgtagccgg gttcaaagag gaagtgaccg
     1621 tggccttcac ggcgctcgaa tccaggagcg ccctgaagat ggatacgccg ctgcgtgatg
     1681 tcccgctcac cgtcaacagc tacaccggct cgttcatgaa gaccatcgag tccacgaacg
     1741 tcggggacct gtacagctac acgaccggcg tgaccaggtc gggaaacacc ggcctggatt
     1801 tcgtgatccg gggcgtgcgt gcctcgcaca ccggcaacat ccagtacaac gggctgccgg
     1861 ggctcgccgc ccgtgtcggc tcaccgtcga ccgtcaacgt cgagcggctg gaggtgttga
     1921 aggggccgag ctcggtgctc tacggccagg cgcagcccgg cggcctgatc aacatcatca
     1981 ccaagaaacc gcaggccgag cgccaggcgc tcctcgacat ccggagcggc ggcacgttcg
     2041 gcggaggcat gaacggtcgc ggcaagcacc acgtgtcaac ggacctgacc gggccgatcg
     2101 acagcgggcg gaagttcctg taccgcttca tcgcatccta tgacgacgag aactcgttcc
     2161 gcgacaacgt gacgaacaag gatctctacc tggtgccgtc gctgtcgtgg atgggctggg
     2221 ctggggcggt gctgaacatc gaggccgagt accgccggac gaacacctcg ctcgacagcg
     2281 ggctggtagc gcccttcaac gacatcaagc tggtggcacc ggccaatgtg cgctaccagg
     2341 aaccgtccga ctacatcgag gaaaccggaa agaccatcac cacgtcgctc aagaagagct
     2401 gggcgaacgg cgtgacgtgg accgcgaact ggcggagtgt gtggcacgag gacctgcagg
     2461 tcggcttcga gaacgccggg acctccggca cccagacagt gacgcgccgc gaccggcggc
     2521 agcggaacct tcgcgagtac cactacgtcg acacgaccat cggccgaagc gtgaagacgg
     2581 ggtcggtcaa ccacaagctg ctgttcggct cgacggtcgg ctacgagatg accgactacg
     2641 accgtcagca gttcgctacc ggtgcggcgc tcaacatcga cctgtaccgt cctgtcttcg
     2701 gggccgccaa cctccccttg aagccgaaca cctggcgcca ttccaccgcc tggaccggcg
     2761 ccggctacct gaacgatcag atcgagctga gcgccatgtg gaaggttctg ctcggcctgc
     2821 gcttcgaggc ccgcagggcc gacgaagagg agttgagggt caaccccaag acccgggaga
     2881 agaacagcca ggcattcctg ccgcttgccg ggctcgtgtt tcagccgatt gattccctgt
     2941 cgctgtacgg cagctactcc acctccttca cgccgccgcc gccgggcgcc atcgacaaga
     3001 acggcgagaa ccccttcaag ccggaaagcg cgcgccagtt cgagtggggc gccaagctcc
     3061 aggtctggaa cggcggggaa ctgtccgtgt cctggttcga catcacgaag aacgatgtcc
     3121 tcatcacgct ggtcgcgcag gccgtcaacg accaaatcgg gcaggagcgc agcaagggac
     3181 tcgagaccac cttcaacgcc cggctgctca acaactggca ggtcatcgtc ggatatgcct
     3241 acaccgactc cgaggtgagc aaggactcgg atccggccaa ggtcgggtcc cggatcccga
     3301 attccccgcg tcacggcgcc aacttctgga cgcgctacga tgtggcgaag ggcgccctga
     3361 gcggcctcgg agccggcctc ggcgtggtgt tcacgggcga acgggcgggg acgatcagcg
     3421 ccagctcgtc gacgctgccg gtcctgctgc tgcccggcta cacccgactc gatctcggcg
     3481 tgtactacgt caagaagcgg tatgagttga ccctgagggt cgtcaatctc accgacaaga
     3541 agtactacga gagcaacctc gggaccacca cgacgtccct gaacatccga ccgggcgctc
     3601 cgcgcagcgg gtcgatgtcc ctgcgcgtga ggttctaggc atggccaacg gagctgcgaa
     3661 gctcgccgcg ctggcagcgg gcgcggccct catcatctcg ggaagcgcgg cgcacgcaca
     3721 ggagggcgcg ttgccgccgt accggcccgt ggccgtctcg tgcgaactca acagcgtggg
     3781 cgacggatcc atggcgccct tgatggatgc cctgttcacg gcctaccaga aggtccagcc
     3841 cggcgtgcgc cagggatcgc gctgggacca ccagcgcgcg gagagggcga tgcccgcgct
     3901 gatgttcgag ctcgccgaga tggcccccct tgcgcgcgag ccccggccgg ccgagcttgc
     3961 gccctacgcc catcagttcg ccggcgacat gatgaaggcg cccctgctgg ttcgcatcgg
     4021 gcgcggcgca gacggcggcg agccggcggt ggcggtcaac aagcgccccg actcgccgct
     4081 tccgccgtgt gtcagggagt tcctggcgtt cgcgctcagc cgcgagggcc aggctctggt
     4141 ggcggcgggc gggcggttcg gcgccctcac ggcagccgag gcggcgcgcg agcgcgagaa
     4201 gctgcaaggt tacctgccag ggctcgatcc gacgctgccc gtctacgccc cggagatcgc
     4261 cgtcagcggt gagatcgcga gcgtgggcag cgacggcatg aagagcctga tggaacggtg
     4321 gatgcgcgac ttccagcgtg tccagccggg cgttcgcaag ggcgagcgct gggaacacct
     4381 ggggactctc aacgggtttc acgccctgat cgccgatgag accatgctgg ctccgatggg
     4441 ccgcgagctg tggccagaag aggccgacgc ttacgcgtcc acacacggcg gcagaaggct
     4501 gctcgagatc cgcgtggcac gcggcgggtt caataccccg cagcggacga cggcccaggc
     4561 catcttcgtc catcagagca acccgctttc gcgcatcacc ctcgggcagg tcgccgcgat
     4621 cttcggcgaa ccggccagca tcacgcgctg gggccagctc ggcttgagcg gcgaatggaa
     4681 agaccgcccg atcaccgtgt atgcgccgcg gctgacggcg cccaacgcca tgtcggtgca
     4741 gctgcgcgtc ctgaacggag ggcgctggaa ggcctcgatc cgcgaaggga cgattgcgga
     4801 gaccgccgcc gccgccgcga gcgaccaggg atccattgcg ttcggcggtt tcgaagaggg
     4861 cggcccggga atcaagcccc tcgcagtggc cgagagcgcg gagggcccgt tctacgaagg
     4921 aaccagcgcg acggtgtcgg gcgggcagta tccgctcgcg cgctacatgt tcatccgcct
     4981 cgaggccgac gagaagcacc tgacgccggc tgtcagggag ttcttccgct tcatcctcag
     5041 ccgcgacggg caggagcccg tccgttactc ggggtacttc ccgctctcga accgggaagt
     5101 acaggaagaa ctgcgcaaac tgcgctgacc gccgccccgg caggccgcgt gccagcccgt
     5161 ggcaccggcc tagccgggag gccgacgccg ggtgcggcgc cccgcggctt cggcaacgac
     5221 gctgccgaag gcggtcacga gcagccacga ggggctatgg cgaggatagc cccttcccgt
     5281 ttcatcctgg agggtgacgg ccgacgcccg tcacccgcgc gctcatcctc cgagcagcgc
     5341 ccttgcggcg tcggcctgtc cggcgcggag cggcagaaac cgtgcgtgct ccagaatgac
     5401 ctgttggcct tcacggctca ggacgaaccg gaggaattcc tcgatcgccg gattcagggg
     5461 cttgccagg
//
LOCUS       DAOSIF010000166         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13412_length_5459_cov_4.190044, whole genome shotgun sequence.
ACCESSION   DAOSIF010000166 DAOSIF010000000
VERSION     DAOSIF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13412_length_5459_cov_4.190044"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..537)
                     /locus_tag="PLT35_06895"
     CDS             complement(40..537)
                     /locus_tag="PLT35_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG28993.1"
                     /translation="MYERIVRQARRHERSLVAFLRDLIAIRSVSGQEGAAVRRVEAEM
                     EACSYDEVRVDAMGNVLGRIGSGPRVLALDAHVDTVDVGDPAAWTVDPFLGAERGGVI
                     YGRGACDMKGALASTVYGGRIIKELGLEGDVTLWVVGSVQEEDCDGLCWQHILGGGGL
                     RPRPW"
     gene            complement(586..1638)
                     /locus_tag="PLT35_06900"
     CDS             complement(586..1638)
                     /locus_tag="PLT35_06900"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HOG28994.1"
                     /translation="MAVNWSSSRRCHRSIVDAIGRTPMVRLGRIPAVDGVRARILVKL
                     EYFSPSGSLKDRVYRNMFERAERRGALKPGMTVLECSTGNAGIGCSFVAAVKGYPCII
                     VMPEGMSEERKKLDLAYGATMVYTPGGESDVDLALRKLEEIRLKNPQRYFVPAQFENP
                     DNVEAHIRTTGPEIWEQTGGRIDAFIMTQGTGGTLTGVGRYLRRRRKTLRLFAIEPAE
                     CPLLSRRTWGPHGIEGIGDGFVPENLDVSLLDGIVTATTGESIEMARRLARDEGIFCG
                     ISSGANVAGAIKLARAHQELKTIVTLICDTGQRYFSTELCGCPKHVDVPEREHPMDAR
                     TVSQLDRYQGRWEIID"
     gene            complement(1679..2047)
                     /locus_tag="PLT35_06905"
     CDS             complement(1679..2047)
                     /locus_tag="PLT35_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOG28995.1"
                     /translation="MARIESYRDLTAWQKAMALAAECYRFTRALPVEERYGLTAQIRK
                     AAVSIPSNLAEGHNRRSRQAFANHVSIALGSQAEVETQIRHCVILQFAPESAATPVLQ
                     LAAEVGRMLHGLVRSLESTD"
     gene            complement(2104..4803)
                     /locus_tag="PLT35_06910"
     CDS             complement(2104..4803)
                     /locus_tag="PLT35_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG28996.1"
                     /translation="MNPDAPAREVSFTLNGAPFTARVDDGTSLLEVLRDQAGLLSPKN
                     GCAPQGSCGACTVLIDGRARASCAVPAARVEGRRVLTLEGFGARERHVFAHAFASAGA
                     VQCGYCLPGIVVAAKRLIEEVPRPTRRQIAEALNNHVCRCTGYVKIVDAIDLAARALQ
                     GEALPALDESGRIGTSLPKQDAARFVLGEHRYVDDIRAEGLLHAAFLFSEHPRARVVR
                     IDTSAARASPGVARVVTAADVPGDRWQGLLERDWPLFVAEGETTHCLGDILAAAVADS
                     ARHAREALRRVLVEYEVLPGVFSPEAALAPGAPRVHPDRSNLLSRSSITRGRVDAAFA
                     ASAHVETRTFTTQVIEHAFLEPESCVAMPGDWGSGAGDRDDEHGSSGVVPISGPRPPA
                     SDPRITVYSQGQGVFDDRRQIASSLGLPESSVHVIQVPTGGAFGGKEDLSIQAQVALM
                     ALLTGRPVKATLTREESIRLHPKRHPMTLTYTVGCGRDGRLTALRARIVGDTGAFASV
                     GAKVLERAAGHAAGPYRVPAVDIESAAVYTNNPPCGAMRGFGANQAAFAIESMLDILA
                     ERAGLDGWEMRWRNALDRGDTFCTGQVLDCSVGLKETLLAVKGVYYSSPRAGIACGIK
                     NVGIGNGAAEAGQAVVEVTDRGRVVIHTGFTEMGQGFHTAMVQCLCDITGIDPALVRT
                     EVDTAFPTPCGMTTASRATVLGGRAVQEAARRLKADLAPGVLLLDLAGRRYYGQHQAG
                     ETMPLGANAARPRTHMTYGFATQVCLLDPEGRLQTVVAAHDVGRIINRRLLEGQIEGA
                     VHMGLGYALTEELALTEGVPDSFRMQSLGLIRARAMPEVRVVLVESCDPEGPFGAKGV
                     GEIGLVPTAGAVANALYRFDGVRRYALPMRDSAAARHMRGHGG"
     gene            complement(4808..>5459)
                     /locus_tag="PLT35_06915"
     CDS             complement(4808..>5459)
                     /locus_tag="PLT35_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG28997.1"
                     /translation="KLGTRLGFWDCLSCSKCVPVCPNGAVFELEVEPFAGEVPVLEVD
                     ARGWRDIGRRGYRVAKTRQIAVFADACNACGNCAVFCPEDGGPHLQKPLFFGSVESWL
                     RAAPRAGFVLSREGAARVLRGRLPVGACALSFVPGSDRAGFEAPGARLSIAWASHAVL
                     DAALWPPLPSSGQSPAHDPHPQPVTVDLSAYLTLRLLLGALLNPARIHYVNAEFVE"
BASE COUNT          795 a         1949 c         1923 g          792 t
ORIGIN      
        1 ggtgcccccg gtagacgccg aggttcgtgg gctcggcaat caccacggcc tcggcctcag
       61 cccgcccccg ccgaggatgt gctgccagca cagcccgtcg cagtcctcct cctggacgct
      121 gccaacgacc cagagcgtga cgtcgccctc gagcccgagc tccttgatga tgcgcccgcc
      181 gtacacggtc gaggcgagcg cccccttcat gtcgcaggcc ccccggccgt agatgacgcc
      241 gccgcgctcg gcgcccagga agggatcgac cgtccaggcg gcaggatcgc cgacatcgac
      301 cgtgtccacg tgggcgtcca gggccagtac gcgcgggccg ctgccgatcc ggccgaggac
      361 gttgcccatt gcatcgacgc ggacctcgtc gtagctgcag gcctccatct ccgcctcgac
      421 gcggcggacg gccgctccct cctggccgct gacggacctg atggcgatga ggtcgcgcag
      481 gaacgcgacc aggctccgct catgacgacg ggcctgccga acgatgcgct catacatctt
      541 cgtctgtcct ccgcccctgg cttcccgagc cccgagccgg ttcccctagt cgatgatctc
      601 ccagcgcccc tggtaacgat cgagctggct gaccgtgcgg gcgtccatcg ggtgctcccg
      661 ctcggggacg tcgacgtgct tcggacaccc gcacagctcc gtggagaagt agcgctgccc
      721 cgtgtcgcag atcagcgtga cgatggtctt gagttcctgg tgcgcgcgcg ccagcttgat
      781 ggcgccggcg acgttggcgc ccgaggaaat gccgcagaag atcccctcgt cgcgggcaag
      841 gcggcgcgcc atctcgatcg actccccggt cgtcgccgtg acgatgccgt cgagcaggct
      901 gacgtcgaga ttctcgggca cgaacccgtc gccgatcccc tcgatgccgt gggggcccca
      961 cgtgcggcgc gagagcagcg ggcactcggc aggctcgatg gcgaacagcc gcagcgtctt
     1021 ccggcgccgt cggaggtagc gtcccacgcc cgtgagcgtg cctccggtgc cctgcgtcat
     1081 gatgaaggcg tcgatgcgcc ccccggtctg ctcccagatt tccggccccg tcgtccggat
     1141 gtgggcctcc acgttgtcgg ggttctcgaa ctgggcgggc acgaagtacc gctgcgggtt
     1201 cttgagccgg atctcctcga gcttccggag tgcgaggtcc acgtcgctct cgccgcccgg
     1261 cgtgtagacc atcgtcgccc cgtaagccag atcgagcttc ttgcgctcct ccgacatgcc
     1321 ttcgggcatg acgatgatgc aggggtagcc cttgaccgcc gccacgaagg aacacccgat
     1381 gcctgcattg ccggtggagc actcgagcac cgtcatgccc ggtttgagcg cgccgcgccg
     1441 ctcggcgcgc tcgaacatgt tccggtaaac gcggtccttc aggctgcccg acgggctgaa
     1501 gtactcgagc ttgacgagga tcctggcgcg tacgccgtcc accgccggga tccggccgag
     1561 ccggaccatc ggcgtgcgcc cgatcgcgtc gacaatcgaa cgatggcagc ggcgcgagga
     1621 ggaccagttc acggccatgg gctgttctcc ataggggaaa gggactaggc gctgggcgct
     1681 agtcggtgct ctcgagggaa cggacaagcc cgtggagcat tcggcctact tcggcggcca
     1741 gctggagcac gggcgtcgcg gcggattccg gcgcgaactg caggatgacg cagtgcctga
     1801 tctgcgtctc gacttcggcc tgcgagccca gggcgatcga tacatgattg gcaaacgcct
     1861 ggcgcgaacg tcggttgtgc ccctccgcga ggttcgaggg aatcgacacg gccgcctttc
     1921 ggatctgggc ggtgagtccg tagcgttctt cgacgggaag cgccctggtg aaccggtagc
     1981 actcggccgc caaagccatc gccttctgcc atgccgtcag gtccctgtac gactcaatcc
     2041 gcgccatgtg tccccgcccg actagcgcct agcgcctagg ccatggtttc cgcccccagc
     2101 cccctatccg ccgtgccccc gcatatgccg cgcggcggcc gaatcgcgca tcgggagtgc
     2161 gtaccgccgc acgccatcga agcggtagag cgcgttcgcg acggcgccgg cggtcgggac
     2221 caggccgatc tcgccgacgc ctttcgcgcc gaacggcccc tccgggtcgc acgactcgac
     2281 gaggacgacg cgcacctcgg gcatggccct tgcgcggatg aggccgagcg actgcatccg
     2341 gaaggagtcc ggcacgccct cggtgagcgc gagttcctcc gtcagggcgt agcccaggcc
     2401 catgtgcacg gcgccttcga tctggccttc cagcaggcgt cggttgatga tccggcccac
     2461 gtcgtgcgcg gccacgaccg tctggagccg cccctcggga tcgagcaggc agacctgcgt
     2521 ggcgaagccg tacgtcatgt gcgtccttgg gcgcgccgcg ttcgccccga gcggcatcgt
     2581 ctcgcccgcc tggtgctggc cgtaatagcg gcgcccggcc agatcgagca gcagcacgcc
     2641 cggcgcgagg tcggccttta gccgcctcgc cgcctcctgg accgcccggc cgccgagcac
     2701 ggtcgcccgc gaagccgtcg tcattccgca cggcgtggga aaggccgtgt ccacctcggt
     2761 ccgcacgagc gccgggtcga tgcccgtgat gtcgcagagg cactggacca tggcggtgtg
     2821 gaagccctgg cccatctcgg tgaagccggt gtgaatgacg acgcggccgc ggtcggtcac
     2881 ctccaccacc gcctgcccgg cttcggcggc gccgttgccg atgcccacgt tcttgatccc
     2941 gcacgcgatg ccggcgcgcg gagaggagta gtagacgcct ttgacggcca gcagcgtttc
     3001 tttcaacccg acgctgcagt cgaggacctg gccggtgcag aaggtgtccc cgcgatcgag
     3061 ggcgttgcgc caccgcatct cccatccgtc gaggccggcg cgctcggcga ggatgtcgag
     3121 catgctctcg atggcgaacg ccgcctggtt cgccccgaag ccgcgcatgg cgccgcacgg
     3181 cgggttgttc gtgtagacgg ccgcggattc gatgtccacg gcggggaccc ggtacggccc
     3241 cgccgcgtgt ccggcggcgc gttcgagcac cttcgcgccc accgaggcga atgcgcccgt
     3301 gtcgccgacg atccgcgcgc gcagggcggt cagccgcccg tcgcgcccgc acccgacggt
     3361 gtacgtcagg gtcatcgggt ggcgcttcgg gtgcaggcgg atgctctctt ctcgcgtgag
     3421 ggtcgccttg acgggccgcc cggtgaggag cgccatcaag gcgacctggg cctggatgct
     3481 caggtcctct ttgccgccga acgctccgcc cgtcggcacc tgaatgacgt gaacgctcga
     3541 ttcggggagt ccgagcgacg aggcgatctg gcggcggtcg tcgaacacgc cctggccctg
     3601 ggagtagacc gtgatgcgag ggtcggaagc cgggggccgg gggccggaga tcggcacgac
     3661 gccggaggag ccgtgctcgt cgtcccgatc cccagcgccc gatccccagt cccccggcat
     3721 cgccacgcac gactccggct cgaggaaggc gtgctcgatg acctgcgtcg tgaaggtcct
     3781 cgtctcgacg tgcgccgagg ccgcgaaggc ggcgtccacc cggcccctgg tgatggacga
     3841 gcgggacagg aggttgctgc gatccgggtg cacgcggggc gcgccggggg cgagcgcggc
     3901 ctcgggtgag aacacgccgg gcagcacctc gtactcgacc aggacgcgcc gaagcgcctc
     3961 ccgcgcgtgg cgcgcgctgt cggcgacggc cgctgccagg atgtcgccga ggcagtgggt
     4021 cgtctcgccc tcggcgacga agagcggcca gtcccgctcg aggaggccct gccagcgatc
     4081 gcccggcacg tccgcggcgg tcacgacgcg cgcgacaccc gggctggcgc gcgcggccga
     4141 cgtgtcgatc cggaccaccc tggcgcgggg gtgctcggag aacaggaacg cggcgtgcaa
     4201 caggccctcg gcgcggatgt cgtccacgta gcggtgctct ccgagcacga agcgcgcggc
     4261 gtcctgcttg gggaggctgg tgccgatccg gcccgactcg tcgagggcag gcagcgcttc
     4321 gccctggagc gcgcgcgccg cgaggtcgat cgcatcgacg atcttcacgt agccggtgca
     4381 gcggcagaca tggttgttca gcgcttccgc gatctgccgg cgcgtgggcc ggggaacttc
     4441 ctcgatgagg cgcttggccg cgaccacgat gccgggaagg cagtagccgc actgcacggc
     4501 gccggctgag gcgaaggcat gcgcgaaaac gtgccgctcg cgcgcgccga agccctcgag
     4561 ggtgagcacg cgccggccct cgacgcgcgc ggcaggcacc gcgcaggacg cacgcgcgcg
     4621 cccgtctatg aggaccgtgc aggcgccgca cgacccttgc ggcgcgcatc cgttcttggg
     4681 cgagagcagg ccggcctgat cccgcaggac ctccaggagc gacgtgccgt cgtccacgcg
     4741 ggcggtgaac ggcgcgccgt tcagcgtgaa tgacacctct cgggccggtg cgtcagggtt
     4801 cacacggtca ctccacgaac tcggcgttga cgtagtgtat ccgcgccggg ttcagcaagg
     4861 caccgagcag cagacggaga gtcaggtagg cggagagatc gaccgtcacg ggctgcgggt
     4921 gggggtcgtg ggctggggac tgaccgctgg acggcaaggg cggccagagc gccgcgtcca
     4981 ggaccgcgtg cgaggcccag gcgatcgaca gccgcgcgcc cggcgcctcg aagccggcgc
     5041 ggtccgagcc cggcacgaac gacagcgcgc acgcgccgac cggcaggcgc ccgcgcagca
     5101 cgcgcgccgc cccttcccgc gagagcacga atccggcgcg gggtgccgcg cgcagccacg
     5161 actcgacact gccgaagaag agcggcttct ggaggtgagg cccgccgtcc tccgggcaga
     5221 agacggcgca gttgccgcag gcgttgcagg cgtcggcgaa caccgcgatc tgcctggtct
     5281 tcgcaacacg gtagccgcgg cgcccgatgt cgcgccagcc ccgcgcgtcc acctcgagca
     5341 ccggcacctc gccggcaaac ggctccacct cgagttcgaa caccgcgccg ttcgggcaca
     5401 caggcacgca cttggagcag gacaggcagt cccagaagcc caggcgggtg ccgagcttg
//
LOCUS       DAOSIF010000167         5446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13450_length_5446_cov_3.718976, whole genome shotgun sequence.
ACCESSION   DAOSIF010000167 DAOSIF010000000
VERSION     DAOSIF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13450_length_5446_cov_3.718976"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLT35_06920"
     CDS             complement(<1..460)
                     /locus_tag="PLT35_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG28998.1"
                     /translation="MTGPVPRLEVERVSKRFGPTTALDEVSLAVAPGEVHAVVGENGA
                     GKSTLMKILSGAVTPDAGAMRLDGEPYRPRTPLDALARGVAMVYQELSLAPHLTVAEN
                     IMLGIEPSRAGFVRRAEGRAAAAAALARLGAREIDLDARAGALSVAAQKMV"
     gene            complement(652..726)
                     /locus_tag="PLT35_06925"
     tRNA            complement(652..726)
                     /locus_tag="PLT35_06925"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(692..694),aa:His,seq:gtg)
     gene            957..1343
                     /locus_tag="PLT35_06930"
     CDS             957..1343
                     /locus_tag="PLT35_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG28999.1"
                     /translation="MPHRFIRPRLLAVAAIAGFALACGGGGASGDLDKIEVTQTQFEI
                     VVNNISGKALMDVLAEIKPAGPSSHYTTRIARLENGEKRGIAHTSFHDRDHVPFSART
                     AKATLIIVSAKDIDGNAIRVELPFKK"
     gene            1544..1620
                     /locus_tag="PLT35_06935"
     tRNA            1544..1620
                     /locus_tag="PLT35_06935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1578..1580,aa:Arg,seq:tct)
     gene            1841..2131
                     /locus_tag="PLT35_06940"
     CDS             1841..2131
                     /locus_tag="PLT35_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29000.1"
                     /translation="MTDRVSTIDVRQRLGDMLNRVALRNDEFIIERKGKALAALVPVE
                     RLEQMRRFARRHALEFLSRQHGGGLTDKQAADLALEAQRWARKSATARTKSA"
     gene            2128..2559
                     /locus_tag="PLT35_06945"
     CDS             2128..2559
                     /locus_tag="PLT35_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOG29001.1"
                     /translation="MTAVLRAVLDANVFVSAAIHPDGPPGRIIEQFLRADAFTLVLSE
                     AIVDEIMRALTYPKVLKYVRRDLDPGLWMEDLAVLADFVLPPAQAHGVSDDPDDDKYL
                     AAAIAGRASLVVSGDPHLLAVEAYEGVRIVTPRASLNLLTG"
     gene            complement(2658..3353)
                     /locus_tag="PLT35_06950"
     CDS             complement(2658..3353)
                     /locus_tag="PLT35_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOG29002.1"
                     /translation="MTSLAIGNDIVDLAEPGAAGKARDRRFMDRVFTAEERACILGAL
                     SPALAVWKTWAAKETAYKIASKVRGKVVFAHRAFEVEPGAAGTAGGWGRVRFDGLDIR
                     VRWETARDYVHCVGQLIQHRPGDAAASPHEPRPVHSAIVHHGQLLSGTLSAAERASVH
                     STPSARARLVARRLFERLGLQGVEVLRHWRERGWGPPVAAIGGEALPGFDVSLSHDGR
                     FVAAAVAGPPPET"
     gene            complement(3331..4083)
                     /locus_tag="PLT35_06955"
     CDS             complement(3331..4083)
                     /locus_tag="PLT35_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG29003.1"
                     /translation="MAPGFDRVGLRVQEVLSHAAAPLTYGALSAWMRFGRRYRIPNLR
                     EVRREFAAQAWDRPGPLLICANHLTLIDSLVIQWAMTSAWRLASRRRAFAWNLPDRQN
                     LARHWWWRVVGYAGKCIPVVRGGTPEQTRRTLDKVAWLLERGQSVLIFPEGGRSRVGR
                     VDREGVSYGVGQILQAVPGARVLCVFLRGRGQEAFSDYPARGETFVVRMERVAPSTSS
                     AGMRGARDLAMQIVGRLSEMEARYFDDLAGHR"
     gene            complement(4090..4347)
                     /locus_tag="PLT35_06960"
     CDS             complement(4090..4347)
                     /locus_tag="PLT35_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOG29004.1"
                     /translation="MDHAAALGKVVAIIKPFAKNTQALEAATDATRIIEDLGVNSARL
                     VDIILAFEDEFGISVDDESADKVHTVGDAVQMILQKTAAGG"
     gene            complement(4406..>5446)
                     /locus_tag="PLT35_06965"
     CDS             complement(4406..>5446)
                     /locus_tag="PLT35_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG29005.1"
                     /translation="KPLLEKMGKSVTLIGDSGAGQVAKACNQILTGVGVLAVAEAFNF
                     AAKSGVDAGKVRRLGSRVVEQTMASSVSALVGGLLALGGQVTTNSSACTTGTEAIVDA
                     FHRIRDGRAVRVMAGGAEGSSHYIWAGFDAMRVLARQSNDRPEAASRPMSATAGGFVP
                     SSGAGLLMVESLSSARARGARIHAEILGGHVNCGGQRGGGTMTAPNPEGARRCILAAL
                     AEAGIGPDAVDAINGHLTATMADPLEIATWQRALGRSPGRFPWINSTKSLVGHGLGAA
                     GGIEAVACVLQLSRGFIHGSINCEDLHPALEPFASRIPHETIAASPTVLAKASFGFGD
                     VNGCLILKTWES"
BASE COUNT          795 a         2005 c         1882 g          764 t
ORIGIN      
        1 ccaccatctt ctgcgcggcg acggagagcg cgccggcgcg cgcgtcgaga tcgatctcgc
       61 gcgcccccag gcgggcgagg gcggccgcgg cggccgcgcg cccctcggcg cggcggacga
      121 acccggcgcg cgaaggctcg atgccgagca tgatgttctc ggcgaccgtg aggtgtggcg
      181 ccagcgacag ctcctggtac accatcgcca cgccgcgcgc gagggcatcc agcggcgtcc
      241 ggggccggta cggctcgccg tcgaggcgca tcgcgcccgc gtcgggcgtc accgcgcccg
      301 acaggatctt catgagcgtg ctcttgccgg cgccgttctc gcccacaacc gcgtgcacct
      361 ctcccggcgc cacggcgagg ctcacctcgt ccagggcggt ggtcggcccg aagcgcttgg
      421 agacgcgctc gacctcgagg cgcggcacgg ggcccgtcat cgcggccgat cgtatcgcat
      481 tcgcgtcgcc gcgcgcggca gcgcccgcgc ccgagcgcag gggcgcgcgc gcgagatcct
      541 gtcgcgcgac ggcgccaccg tcgagcgcat cgtcgggcgc gacggcaact ggaacaacgg
      601 gcaggcccgg cgggacggct cgaggtcagg tggcggagcg ggatggagga gtggggtggg
      661 taacggggtt cgaacccgcg acttccggag ccacagtccg gcgctctgcc actgagctat
      721 acccaccgcg ccgcaagact tcactttacc acagggccgc agccgcgccg ggcggggcgg
      781 gagccccgcg cggcggcggc ttgactccgg cagcggctcg gcctacattg cagtcgcctc
      841 ctcgcaggag cgccggacgc cggctgcctc ccgcgcccgg gaaggccgta tccggcccgg
      901 atgagtgtcc tctgcgccgg cggaaggccg atagcccgac gaaaggagca ccgcccatgc
      961 cgcaccggtt catccgccca cgactcctcg ccgtcgccgc gattgccggg ttcgccttgg
     1021 cctgcggggg cggcggcgcg agcggggatc tcgacaagat cgaggtgacg cagacgcagt
     1081 tcgaaatcgt ggtgaacaac atctcgggca aggccctgat ggacgtgctg gcggagatca
     1141 agcccgccgg tccgtccagc cactacacga cgcgcatcgc caggctggag aacggggaga
     1201 aacggggcat cgcgcacacg agtttccacg accgggacca cgtgcccttc agcgcccgca
     1261 cggccaaggc gacgctcatc atcgtgagcg cgaaggacat cgacgggaac gcgatccgcg
     1321 tcgagctgcc gttcaagaag tgaggggcgg ccgccccata cggcccttcg acgcgctgag
     1381 ggaccgcccg agcagcgtcg agaggcgact cgccggtttc cggacctgat gctgcccccg
     1441 tcggctcgtg aggagccgtc tccacctccc cggccccgag gctgcccaag ggccggcgtg
     1501 ccggcggccg gttcgtcgta caatggcccg tcaccgccgg agcgcgcccg tagctcagct
     1561 ggatagagca ccggccttct aagccggggg ccccgcgttc gaatcgcggc gggcgcgcca
     1621 gccttcgcga gggcccggct cgatcctttc ctcccttcgc ttcggccagg caggcctctt
     1681 tcggagccac gtcaccgatg aagccccggg gcttccgcct tcgcgcgaag cttcggcgga
     1741 ctgcggccgc ggcaaccgcg gtcagcttcg gcatcgcctg aggtccagag aatcattgcg
     1801 attgcatcca gagcaactat aatagttgcg agggtcccac atgaccgata gggtgtccac
     1861 catcgacgtg cggcagcggc tcggcgacat gctcaaccgg gtggcgcttc ggaacgacga
     1921 gttcatcatc gagcgaaagg ggaaggccct cgccgcgctg gtgccagtcg agcgcctcga
     1981 acagatgcga cgcttcgctc gccgccacgc attggagttc ctctcccggc agcatggcgg
     2041 cgggctgacc gacaagcagg ccgccgacct ggccttggag gcgcagcgct gggcccgtaa
     2101 gtccgccaca gcgcgaacga agtcggcgtg acggccgtgc ttcgtgccgt cctcgacgcc
     2161 aacgtgttcg taagcgcggc catccatccg gacggcccgc cgggccgcat tatcgagcag
     2221 ttcctccgtg cagacgcgtt tacgctggtt ctgtctgagg cgatcgtcga cgagatcatg
     2281 cgggccctca cgtacccgaa ggtcctcaag tacgtgcgca gggaccttga ccccggactg
     2341 tggatggagg acctggccgt cctcgccgac ttcgtgctgc ctcctgccca ggcgcatggc
     2401 gtgagcgacg acccggatga cgacaagtac ctagccgccg ccatcgccgg ccgggcgtcg
     2461 ctggtagtga gcggcgatcc tcacctcctg gcggtcgagg cgtatgaagg cgttcgcatc
     2521 gtcacgccgc gggcgtctct caacctgctg acaggttgag cacgtcgtcc gggcggtcag
     2581 cagcacgacg tgcggcggcc cgactcgggt gcggcctctg cctcggcgat cgaacgagac
     2641 tggaggacga cgcggggcta cgtctccggc ggcggcccgg cgacggccgc cgcgacgaaa
     2701 cggccgtcgt gagacaggct gacatcgaag ccgggaagcg cttcgccccc gatcgcagcc
     2761 acgggcggac cccagccccg ttcccgccag tggcgaagca cttccacgcc ctgcaggccc
     2821 agccgttcga acagccggcg cgcgacgagc cgggcgcggg ccgacggcgt cgagtgcacg
     2881 gacgcccgct cggccgccga cagcgtgccg ctcaggagct ggccgtgatg gacgatggcg
     2941 gagtggacgg gccgcggctc atgcggcgac gccgcggcgt cgccgggacg gtgctggatt
     3001 agctggccga cgcagtgcac gtagtcgcgc gccgtctccc agcggacgcg gatgtcgagg
     3061 ccgtcgaaac ggacccgccc ccatccgccg gccgtcccgg cggcgccggg ctccacctcg
     3121 aacgcgcgat gcgcgaagac gaccttgccg cggaccttgc tcgcgatctt gtaggcggtc
     3181 tccttggcgg cccacgtctt ccacacggcc agcgccggcg agagggcccc gagaatgcag
     3241 gcgcgctcct cggccgtgaa cacgcgatcc atgaaccggc ggtcgcgcgc cttgcccgcc
     3301 gcgccgggct cggccaggtc gacgatgtcg ttaccgatgg ccagcgaggt catcgaagta
     3361 tcgcgcctcc atctcactca gccgtccgac gatctgcatc gccaggtcgc gcgccccccg
     3421 catccctgcc gacgacgtag acggcgcaac gcgttccatc cggacgacga acgtctcgcc
     3481 gcgggcgggg tagtcgctga acgcctcctg cccgcgcccg cggaggaaca cgcacaggac
     3541 gcgcgcgccg ggcacggcct gcaggatctg gcccacgccg tacgacacgc cttcgcgatc
     3601 cacccgcccg acgcggcttc gcccgccctc cgggaagatc agcaccgact gcccgcgctc
     3661 gaggagccac gccaccttgt cgagcgttct cctcgtctgc tcgggcgtgc ccccgcgcac
     3721 gacggggatg cacttgccgg cgtacccgac gacccgccac caccagtgcc tggccaggtt
     3781 ctgccggtcc gggaggttcc acgcgaaggc gcgccgccgg ctcgcgagcc gccaggccga
     3841 cgtcatcgcc cactggatga cgagcgaatc gatgagcgtc aggtggttcg cgcagatgag
     3901 cagggggccc ggcctgtccc aggcctgcgc cgcgaactcc cgtcgcacct cgcgaaggtt
     3961 cgggatccgg taccgccggc cgaacctcat ccaggccgac agggcgccgt aggtcagggg
     4021 ggccgcggcg tgcgacagca cctcctgcac gcgcagcccg acccgatcga accccggcgc
     4081 cacccccggc taacctccgg ccgcggtctt ctgcaggatc atctgcacgg cgtcgccgac
     4141 ggtgtgcacc ttgtccgcgg actcgtcgtc cacggaaatg ccgaactcgt cctcgaacgc
     4201 caggatgatg tccacgaggc gggccgaatt cacgcccagg tcctcgatga tccgcgtcgc
     4261 gtcggtggcg gcctcgagcg cctgcgtgtt cttcgcgaac ggcttgatga tggcgacgac
     4321 cttcccgagc gctgctgcgt ggtccattcc cgtctcctct atcggtggcc ggccgccggc
     4381 ggtcgtgccg cggcggccgc ccgggctacg actcccaggt cttcagaatc aggcatccat
     4441 tgacgtcgcc gaagccgaag ctggccttgg ccagcaccgt gggcgaggcg gctatcgtct
     4501 cgtgcgggat cctcgacgcg aacggctcga gggcgggatg cagatcctcg cagttgatcg
     4561 agccgtgaat gaagccgcgg ctgagctgca gcacgcacgc gaccgcctcg atgccgcccg
     4621 ccgctccaag gccgtggccg acgagcgact tggtggagtt gatccacggg aagcggcccg
     4681 ggctccggcc gagcgcccgc tgccaggtgg cgatctcgag cgggtcggcc atggtggccg
     4741 tcaggtgccc gttgatggcg tccaccgcgt cgggcccgat gccggcctcg gccagggccg
     4801 cgaggatgca gcgccgcgcg ccttcggggt tcggcgccgt catggtgccg ccgccccgct
     4861 ggccgccgca gttgacgtgg ccgccgagga tctccgcgtg aatccgcgcg ccgcgcgccc
     4921 gcgccgacga caggctctcc accatcagca ggccggcccc cgacgagggc acgaagccgc
     4981 cggccgtggc gctcatgggc cgcgaggccg cctcgggacg gtcgttcgac tggcgggcca
     5041 gcacgcgcat cgcgtcgaac ccggcccaga tgtagtgcga cgagccctcc gccccgccgg
     5101 ccatcacgcg cacggcccgt ccgtcccgga tccggtggaa cgcgtccacg atggcttcgg
     5161 tcccggtggt gcacgcgctg ctgttggtgg tcacctggcc gccgagcgcc agcagtccgc
     5221 cgaccagcgc gctcaccgaa ctcgccatcg tctgttcgac cacgcggctt cccagtcggc
     5281 gcaccttgcc ggcatcgacg ccgcttttcg ccgcgaagtt gaaggcctct gcgacggcaa
     5341 gcacgccaac gccggtgagg atctggttgc aggccttggc cacctgcccg gcgcccgagt
     5401 cgccgatcag cgtcactgac ttgcccattt tttccagcag cggctt
//
LOCUS       DAOSIF010000168         5445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13452_length_5445_cov_4.770315, whole genome shotgun sequence.
ACCESSION   DAOSIF010000168 DAOSIF010000000
VERSION     DAOSIF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13452_length_5445_cov_4.770315"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..451
                     /locus_tag="PLT35_06970"
     CDS             89..451
                     /locus_tag="PLT35_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG29006.1"
                     /translation="MTILLAEDEPDVQLIARLSLKKAGFTVVTAGNGVEALERVAAER
                     PDVILLDWMMPDMDGFETCRRLKADPATAGIPVIFLTARVQEAEVARAMALGAAGCIG
                     KPFDALTLGQRVLEFVAG"
     gene            452..2272
                     /locus_tag="PLT35_06975"
     CDS             452..2272
                     /locus_tag="PLT35_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29007.1"
                     /translation="MTIRSRIFIVVGVVIAAAFAQTLLVLRIEGRRGEISAAVDRALW
                     RVEQQASLGRLVAELDGVERARAGMGSAAYGEEHGRLWTLYERSVALLPQYMDDDEAR
                     RELSAIDARLRAWHEAASGAPAAGQGAVLIQAIRGDLDRLETRERNRLAAERARANSQ
                     SLQSTLLTLAIPAAAIVMLLALLAIIGRILLDPLAAVATSARQISAGTFDVTLPRESR
                     DEIGDMARAFREMIAAVERRQREAAESLARAEREHRRLQATIETVPVGLLIVDAETGR
                     VALQNQAATRLIGRQPGAGSAWLAYWDAFRVTTREGAPVPLRQWAATRLTRGVVGEEL
                     VVKQADGRETPILVSAAPLGEEDGRISGAVVAFQDITSLYEVDRLKSEFVSIVSHELR
                     TPLTSIKGAIQLLLDAGLAADADHLMLMNVALANTDRLVRIINDILDISKIEAGKLEL
                     APKPHAAADVAAVSVQNVAQIADASRIALASRIEDGVPPVVVDLDRMVQILVNLLSNA
                     LKAAPAGSEVTLAVRRAGDGFAAFSVTDCGPGIPPEKIELLFQKFQQLDGANTRKARG
                     TGLGLAIVKALAEMQGGRVEVQSEVGRGSTFTVTVPTARG"
     gene            complement(2410..4956)
                     /locus_tag="PLT35_06980"
     CDS             complement(2410..4956)
                     /locus_tag="PLT35_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG29008.1"
                     /translation="MSKQLESLLRQAGLATDAQFGRVRNRMEQDAGSFLEILVKEEHV
                     SEEAIADAIAERTKVPRVRVATFLLDEEALRRVPERLARKLECLPLAVEGRALIVAMA
                     DPTDYRAIQDIEFTAAMPVRPVVATLSEVTSGIGERYGADDRIKSFLANVSEAPDLQI
                     VSEDQHELSLDAADSAAAAEVAPVVKMCSVIVFDAIRSQASDVHIEPALNDLQVRMRV
                     DGVLRDYTRVPKWLHGPVVSRLKILAKLDIAERRIPQDGRVNVLYQGRSIDLRVSTLP
                     THFGEKMVLRVLGGAKAPDISALGLSKPQEALLQSSISQPQGMILVTGPTGSGKTTTL
                     YALLAWHHKPEVNIVTVEDPIEYQLAGINQVQINPKAGLTFASALRSILRQDPDVILV
                     GETRDRETAEIAFQAAMTGHMVLSTLHTNSAVGAVSRMYDLGVDPAILATSLTMVAAQ
                     RLVRRICGECREEYEPGEAVRNLVGLFGDASPVFRGRGCPACGGTGFAGRVGIYELFR
                     PTNAVRKLINDRASEAEIRYAARQSGMTLLREDALEKIKAGITSPDEVLRVVQVDESE
                     IPCPACKAIIEADFATCPYCRTSLKRNCAGCGQPLRREWKVCPHCNTTVAMQLLAEEL
                     GMAERKPAEAPPQAAAEAAAEDSLPFTPMIDLESAGLAAADQPAGLAAADQPAGLAAA
                     DQPAESTAGATTPPAAAAAPAEESAEAQVPTVDPRGGQRPLRVLIVDDDPDIRMIVRA
                     TLRGLTVPVESVQAANGIEALEQAKAAKPDLVILDVMMPEMDGFETCRALRADLRTAF
                     VPILMLTASADQDSRAKGYLIGTDDYMSKPFLPLDLKLRVSRLLRKTYGV"
     gene            complement(5212..>5445)
                     /locus_tag="PLT35_06985"
     CDS             complement(5212..>5445)
                     /locus_tag="PLT35_06985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG29009.1"
                     /translation="NASGEDFGSERLVAAVCRNRERPAQAIADAIFEAVDDFRGGAEQ
                     HDDRTVVVLKITGQEQESRSRERTGGRRSNRVR"
BASE COUNT          749 a         1898 c         1955 g          843 t
ORIGIN      
        1 cggagctgct ggtgcgcatc cagctgctcc tggcgcggcg ccggacggcg gaagctggcg
       61 ccggggccgt ggctctgcag gactcccgat gacgattctc cttgccgaag acgaaccgga
      121 cgtgcagctg attgcccgct tgtctttgaa gaaggccggg tttaccgtgg tcaccgcggg
      181 aaacggcgtg gaagcgctcg agcgcgtggc cgccgagcgc cccgacgtga ttctgctcga
      241 ttggatgatg cccgacatgg acgggttcga gacgtgccgg cggctcaagg ccgacccggc
      301 cacggcgggc atccccgtga tcttcctgac agcgcgtgtg caggaggccg aggtggcccg
      361 cgcgatggcc ctcggggcgg ccggctgcat cggcaagccg ttcgacgcgc tgacgctcgg
      421 ccagcgcgtc ctggagttcg tggcgggcta agtgacgatt cgctcccgca tcttcatcgt
      481 cgtcggcgtt gtcatcgccg ccgcgtttgc gcagaccctg ctggtgctcc ggatcgaggg
      541 ccgccgcggg gagatctccg ccgcagtgga tcgggcgctc tggcgggtcg agcagcaggc
      601 gtcgctcggc cggctcgtgg ccgaactcga cggcgtcgag cgggctcgcg cgggaatggg
      661 cagcgccgcg tacggcgaag agcacggccg cctgtggacg ctgtacgagc ggagcgtcgc
      721 gctgcttccc cagtacatgg acgatgacga ggcgcggcgg gaactgtcgg ccatcgacgc
      781 caggctgcgc gcgtggcacg aggcagcgtc gggcgcgccg gccgccggac aaggcgcggt
      841 cctcatccag gccatcaggg gggatctcga ccggctcgag actcgcgaac gcaaccggct
      901 cgccgccgag cgggcccggg ccaacagcca gtccctgcag tccacgctgc tgacgctcgc
      961 cattcccgcg gctgccatcg tgatgctgct ggcgctgctg gcgatcatcg gccggatcct
     1021 gctggatccg ctggcggccg tcgccacgtc ggcgcggcag atctcggccg ggacgttcga
     1081 cgtgacgctc cctcgggagt cgcgcgacga gatcggcgac atggcccggg ccttccgcga
     1141 gatgatcgcc gccgtcgagc ggcggcagcg ggaggcggcc gaatcgctcg cgagggccga
     1201 gcgcgagcac cggcggctgc aggcgacgat cgagaccgtg cccgtcggcc tgctcatcgt
     1261 cgatgccgaa acgggccggg tcgcgctgca gaaccaggcc gcgacccggc tgatcggtcg
     1321 gcagcccggt gccgggtcgg cgtggctcgc ctactgggac gccttccgcg tgacgacccg
     1381 cgagggcgcg ccagtgcccc tgcgccagtg ggccgcgacg cgattgacga ggggcgtcgt
     1441 tggcgaagaa ctcgtcgtga agcaggcgga cgggcgcgag acgcccattc tcgtcagcgc
     1501 cgccccgctc ggcgaggagg acggccggat ttccggcgcc gtcgtggcct tccaggacat
     1561 cacgagcctc tacgaagtgg accgactcaa gagcgagttc gtgtcgatcg tgtcccacga
     1621 gctgcggacg ccgctcacct cgatcaaggg cgcgatccag cttctgctgg acgcggggct
     1681 ggccgccgac gccgaccacc tgatgctcat gaacgtggcc ctggcgaaca ccgaccgtct
     1741 cgtgcgcatc atcaacgaca tcctcgacat ctcgaagatc gaggcgggca agctcgagct
     1801 cgcgccgaag ccccacgccg cggcggacgt tgccgcggtg tcggtgcaga acgtggcgca
     1861 gatcgccgac gcgtcccgga ttgcgctcgc ctcgcgcatc gaagacggcg tgccgccggt
     1921 cgtcgtcgat ctcgaccgca tggtccagat cctcgtgaac ctcctctcga acgcgctgaa
     1981 ggcggcgcct gccgggtccg aggtgaccct ggccgtgcgc cgcgccggcg acgggttcgc
     2041 ggcgttctcg gtgaccgact gcggcccggg catcccgcca gagaagatcg agctgctctt
     2101 ccagaagttc cagcagctgg acggcgcgaa cacgcgcaag gcccgcggca ccggcctcgg
     2161 cctcgccatc gtgaaggccc tcgccgaaat gcaggggggg cgggtcgagg ttcagagcga
     2221 ggtgggccgg ggatcgacct tcaccgtcac ggtgcccaca gccagaggct agctaccagg
     2281 agctggcgcg aggggctagg gactgggtgc gaggggctag tcggacgcgg ctgtcgccgc
     2341 gtcggaggca tgactgttcg gcctgtcggc tgactggccc ccagcccctg gtccccagcg
     2401 cctggctccc tagaccccgt acgtcttccg cagcagccgg ctgacgcgca gcttgagatc
     2461 gaggggcagg aacggcttgg acatgtagtc gtcggtgccg atgaggtagc ccttcgcgcg
     2521 gctgtcctgg tccgcgctcg cggtcagcat cagaatgggg acgaacgccg ttcgcaggtc
     2581 ggcgcggagc gcgcggcacg tctcgaagcc gtccatctcg ggcatcatca cgtcgaggat
     2641 caccaggtcc ggcttggccg ccttcgcctg ttccagggcc tcgatgccgt tcgcggcctg
     2701 cacgctttcg accggcaccg tcaggccgcg cagcgtggcc cgcacgatca tcctgatgtc
     2761 cggatcgtcg tccacaatca gcacgcgcag cgggcgctgg ccgccgcgag gatcgacggt
     2821 cggcacctgg gcctcggcgc tctcttcggc gggagccgcc gcggcggccg gcggcgttgt
     2881 cgcgcctgcg gtcgactcgg ccggctggtc cgccgccgcc agaccggccg gctggtccgc
     2941 cgccgccaga ccggccggct gatccgccgc cgccagaccg gccgactcca ggtcgatcat
     3001 gggggtgaac ggcaggctgt cctcggctgc ggcctcggcc gccgcctgcg gcggcgcctc
     3061 ggccggtttc cgctcggcca tcccgagttc ttcggccaga agctgcatcg ccaccgtcgt
     3121 gttgcagtgg gggcacacct tccactcccg ccggagcggc tgtccgcagc ccgcgcagtt
     3181 cctcttgagg ctggtccggc agtaggggca cgtggcgaag tcggcctcga tgatggcctt
     3241 gcaggccggg cacgggatct cgctctcgtc cacctggacg acgcgcagga cttcgtctgg
     3301 cgacgtgatg ccggccttga ttttctcgag ggcgtcctcg cgcagcagcg tcatgccgga
     3361 ctggcgggcg gcgtagcgga tctcggcctc gctggcccgg tcgttgatca gcttgcggac
     3421 cgcgttcgtc ggcctgaaca gctcgtagat gccgacgcgc ccagcgaagc ccgtgcctcc
     3481 gcaggcgggg cagccgcgcc cgcggaacac gggcgacgcg tcgccgaaga ggcccacgag
     3541 gttgcggacg gcctcgccgg gctcgtactc ctcccggcac tcgccgcaga tccgccgcac
     3601 gaggcgctgg gcggcgacca tcgtgagcga ggtcgccagg atcgcgggat cgacgcccag
     3661 atcgtacatg cgcgagacgg cacccacggc gctgttcgtg tggagcgtgc tcagcaccat
     3721 gtggcccgtc atggccgcct ggaacgcgat ctcggcggtc tcgcggtcgc gggtctcgcc
     3781 gacgagaatc acgtcggggt cctgcctcag gatcgatcgc agcgcgctgg cgaaggtcag
     3841 ccccgccttg ggattgatct gcacctggtt gatgcccgcc agctggtact cgatcggatc
     3901 ctcgacggtg acgatgttga cttcgggctt gtggtgccag gcgaggaggg cgtacagggt
     3961 cgtcgtcttg cccgacccgg tgggaccggt gaccaggatc atgccctgcg gctggctgat
     4021 cgacgactgg agcagcgcct cctgcggctt gctcagtccg agcgccgaga tgtccggcgc
     4081 cttcgcgccg ccgagcacgc gcagcaccat tttctcgccg aagtgcgtgg gcagcgtgga
     4141 gacgcgcagg tcgatggagc ggccctggta gaggacgttc acccggccgt cctgggggat
     4201 gcggcgctcg gcgatgtcga gcttggcgag gatcttcagc cgcgacacca ccgggccgtg
     4261 gagccacttc ggcacccgcg tgtagtcgcg cagcacgccg tccacccgca tgcgcacctg
     4321 caggtcgttg agggcgggct cgatgtggac gtcgctcgcc tgcgacctga tcgcgtcgaa
     4381 gacgatgacg ctgcacatct tcacgaccgg agccacctcg gcggcggccg ccgagtcggc
     4441 ggcgtcgagc gacagctcgt gctggtcctc ggacacaatc tggaggtccg gcgcctccga
     4501 cacgttggcc aagaacgact tgatgcggtc gtcggcgccg tagcgctcgc cgatgccgct
     4561 tgtgacttcc gacagcgtgg cgacgaccgg cctgaccggc atggcggccg tgaactcgat
     4621 gtcctggatg gcccggtagt cggtgggatc ggccatcgcg acaatcagcg cccggccttc
     4681 gacggcgagg ggcaggcact cgagcttgcg ggccagccgt tcgggcaccc tgcgcagcgc
     4741 ctcctcgtcc agcaggaacg tggcgacccg cacccgcggc accttcgtcc gctcggcgat
     4801 ggcgtcggcg atggcctcct cgctgacgtg ctcctccttc acgaggattt ccaggaagga
     4861 tccggcgtcc tgttccatcc gattccggac ccgtccgaac tgggcgtccg tggccaggcc
     4921 ggcctgacga agcagagact cgagctgttt ggacatggtc gccgccactg ccaccggtag
     4981 aggtcacccg ggcgtccctg ccgcaggacg ctgcggaggt gtatcggccc gaaacgcctg
     5041 acacgtgagg gcccgccggc ggcacgcgtc ttgatgggag aaggggcagg ccgcgtcgag
     5101 tcagcggggc gggtcaccaa caggcaggcg ctggcatcgc cgccccgccg ggcagatcga
     5161 ttcggcgcgg ctcccctcct ccattccccg gggcggcgct cgggccgagc gtcatcggac
     5221 gcggttcgag cgccgtcccc ctgtccgttc ccggcttcgg ctctcctgct cctgcccggt
     5281 gatcttcagc accacgacgg tccggtcgtc gtgctgctcg gcgccgccgc ggaaatcgtc
     5341 caccgcctcg aagatcgcat cggcgatggc ctgcgcgggc cgctcgcggt tccggcacac
     5401 ggcggcaacg aggcgctcgg acccgaagtc ctcgcccgag gcgtt
//
LOCUS       DAOSIF010000169         5444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13454_length_5444_cov_4.262757, whole genome shotgun sequence.
ACCESSION   DAOSIF010000169 DAOSIF010000000
VERSION     DAOSIF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13454_length_5444_cov_4.262757"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3462)
                     /locus_tag="PLT35_06990"
     CDS             complement(<1..3462)
                     /locus_tag="PLT35_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29010.1"
                     /translation="MRELLEMAFDGFDGARESGEGAELLGLERLEEHARRLAALLTPE
                     RRRRSGRPEHLKRLGEDARALRRIYTALEEDARAGEATPPAAEWLLDNFHVIAAAIRD
                     VRSDLPPSFYRRLPSLTADEFAGLPRVSAMARELIRSSAGRLDAHRIQRFMTAFQSVT
                     PLTIGEIWAWPSALKLALVEQLRARAEVIFAARSQRLDADRLAGTLDSPRAEPRAWPD
                     PFHPAFVIRVLQRSREHGAAAAALRQELGAELDARGQTIEEVIRAEAQYQAASQAAMA
                     NLVESLRLVSTFDWSEFFESVSLVEQVLLRDPSAVYGRMDFRSRDRYRHAVEELAEPT
                     GEGQVRVALKAVERARQVAERDREAREAHVGYYLIGGGRPKFEQGIGYTPGPWQRLRR
                     RAFRHATPLYLGAIALVTVSILAALAAYSQRHGWGAAGAVMVMLLALVPASELAVQIV
                     QQALGRLIPPRRLPRLDLQQVPDSACTMVIVPTLLDSVDAVRDLLSHLEVQALGNVDP
                     NVHFAILSDFRDAPTECLPRDAELLEAARSGIEELNAQYAPEANDRFFLFHRVRQWNP
                     QENIWMGWERKRGKIEEFNRLLRGATDTSFVLHVGNLAVLPKVRYCITLDSDTRLPRG
                     AARELIGIVSHPLNRPRFDPEARRVVDGYGILQPRVSVTFASAEGSLFARLFAGHTGV
                     DPYTTAVSDTYQDLFGEGIFTGKGLYDVDAFTSALEDVVPENALLSHDLFEGLHARVA
                     LVSDVEVVDDYPSTVLSHARRQHRWVRGDWQILLWLFPFVPSRRGLVRNTLPLIGRWK
                     ILDNLRRSLVRPTLMALLLAGWTVLPGSHWLWTGVVFAVVAAQLLPLVARLVTGPRRA
                     QSLPVFLRNLRNDIALALARATLGISLLAYHAFLSAHAIGLTLVRLAFTRKRLLEWET
                     AAAVAARTAGLTTGKALRRYHVEMASSPIAAVASAFVVAAVNPSALPAALPFLTLWLL
                     APAIGYWIGLPAAARVEPLSAAERLALRRVARKTWRFFETFVTQDDAWLPPDNFQVDG
                     AGPKLARRTSPTNIGLGLLSTLAAYDLGYIAAGELADRIDRTLTTLEGLEQHEGHFLN
                     WYDTATLAPLHPHYVSTVDSGNLAASLIALVQGLRQAADEPQTPAQRLEGLADTADLL
                     CEASGLQG"
     gene            complement(3459..3932)
                     /locus_tag="PLT35_06995"
     CDS             complement(3459..3932)
                     /locus_tag="PLT35_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG29011.1"
                     /translation="MPAHAIGLTLGERLARWLTLEQPALRAVVPEAVELVEAVTRAPF
                     WAKARARSEVHAEVPFAVRVEAGTSIPGVAVVERPAVLRGVIDLVYRAADGWRILDYK
                     TDRLEGAEDRLAGRRRGRTASSRSTRSAGRRRGGRRATSWRRRARLPRRPRVRSS"
     gene            4097..>5444
                     /locus_tag="PLT35_07000"
     CDS             4097..>5444
                     /locus_tag="PLT35_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOG29012.1"
                     /translation="MFPLAAVLLLVVMEVPALIWGQQPAPPTGQQCVDCHLKNSPQLV
                     ADWRASKHFEAGIGCETCHPAEHIADADKGVLVKRGNALPTPELCAQCHADRVDQFKK
                     GKHAMAWTVMKAMPTAHMLPATLSDGMKGCGGCHKIGTKTDAEIRELRKTGVGYGVAS
                     CDACHTRHTFSVAEARQPEACKTCHMGFDHAQWEMYSSSKHGVRQQLRQMKVLPEEAA
                     APTCQTCHMPGGDHGVRTAWGFLAVRLPMPEDKQWAEDRTVILQALGVLDASGKPTAR
                     LDTVKAADLARLTQESWQQERDAMQKACASCHVQSFAKAELAKGDDMIRQADRLMAEA
                     IRTVAALYADGVLKKPSAADSPYPDLLEFHDASSQIEQKLFVMFLEHRMRAFQGTFHA
                     SPDYALWYGWSELKRDVPMPPGAELLLHGDGRIGTRRRRGEVVRAGVDRLVLRQRPG"
BASE COUNT          812 a         1891 c         2028 g          713 t
ORIGIN      
        1 cccctgcagc cctgacgcct cgcacagcag gtcggccgta tcggcaaggc cctccaggcg
       61 ctgggccggc gtctgcggct cgtcggccgc ctgtcggagg ccctgcacga gcgcgatcag
      121 cgacgcggcg aggttgccgc tgtccaccgt cgagacgtaa tgcggatgga gcggcgccag
      181 ggtggcggtg tcgtaccagt tgaggaaatg cccctcgtgc tgctcgagcc cctcgagcgt
      241 ggtcagcgtg cgatcgatgc ggtcggccag ctcgccggcc gcgatgtagc ccaggtcgta
      301 ggcggcaagc gtcgagagca gccccagccc gatgttggtg ggcgaggtgc gccgtgccag
      361 cttcggcccg gcgccgtcca cctggaagtt gtccggcggc agccaggcat cgtcctgggt
      421 gacgaacgtc tcgaagaacc gccaggtctt gcgggccacg cgccgcagcg cgaggcgctc
      481 ggcggccgag agcggctcca cgcgggcggc agccggaagg ccgatccagt acccgatggc
      541 cggcgcgagc agccagagcg tcaggaaggg caaggccgcg ggcagcgcgg acgggttgac
      601 ggcggccacc acgaaggccg aggccaccgc ggcaatcggg ctcgacgcca tctcgacgtg
      661 gtagcggcgg agcgccttgc cggtggtgag cccggcggtc cgcgcggcga ccgcggccgc
      721 ggtctcccac tcgaggagcc gcttccgggt gaaggccagg cgcaccagcg tcagcccgat
      781 ggcgtgtgcc gagaggaaag cgtggtaggc gagaagggag atcccgagtg tggcccgcgc
      841 gagcgcgagg gcgatgtcgt tgcgcaggtt gcggaggaac accgggagcg actgcgcgcg
      901 gcggggcccg gtgacgaggc gggcgacgag cggcagcagc tgcgccgcga cgaccgcgaa
      961 gacgacgccg gtccacagcc agtggctgcc gggcagcacc gtccagccgg cgagcagcag
     1021 cgccatcagc gtcggccgga cgaggctgcg ccgcaggttg tcgaggatct tccagcggcc
     1081 gatgagcggc agcgtgttcc gcacgaggcc gcggcgcgag gggacgaagg ggaacagcca
     1141 gaggaggatc tgccagtcgc cgcgcaccca gcggtgctgg cgccgggcgt gggagagcac
     1201 cgtggacggg tagtcgtcca ccacctcgac gtccgacacc agcgctacgc gcgcgtgcag
     1261 accctcgaac aggtcgtgcg acagcagcgc gttctcgggg acgacgtcct cgagcgccga
     1321 ggtgaacgcg tccacgtcgt acagcccctt gccggtgaag atgccttcgc caaaaaggtc
     1381 ctggtaggtg tccgagaccg ccgtcgtgta gggatcgaca ccggtgtgcc cggcgaacaa
     1441 ccgggcgaag agcgacccct cggcgctcgc gaacgtgacg ctgacgcgcg gctgcaggat
     1501 gccgtagccg tccaccaccc ggcgggcctc cgggtcgaag cgcgggcggt tcagggggtg
     1561 gctgacgatg ccgatcagct cgcgggccgc gccgcgcggc aggcgcgtgt ccgagtcgag
     1621 cgtgatgcag tagcggacct tcggcagcac ggcgaggttg ccgacgtgga gcacgaagct
     1681 cgtgtcggtg gcgcctcgca ggagccggtt gaactcctcg atcttccccc gtttccgctc
     1741 ccagcccatc cagatgttct cttgggggtt ccactggcgc acgcggtgga acaggaagaa
     1801 ccggtcgttg gcctcgggcg cgtattgcgc gttcagctcc tcgatccccg agcgcgccgc
     1861 ctccagcagc tcggcgtcgc ggggcaggca ctcggtgggg gcgtcgcgaa aatcgctgag
     1921 gatggcgaag tggacgttgg gatcgacgtt gccgagcgct tgcacctcga ggtgcgacag
     1981 caggtcgcgc accgcatcca cgctgtcgag cagcgtgggc acgatgacca tcgtgcacgc
     2041 cgagtcgggc acctgctgca ggtcgaggcg cgggagccgg cgcggcggga tcaggcgccc
     2101 gagggcctgc tgcacgatct gcacggccag ctcgctcgcc gggacgagcg cgagcagcat
     2161 gaccatcacc gcgccggcgg cgccccagcc gtgccgctgg gagtaggcgg caagcgccgc
     2221 gaggatcgac acggtgacga gcgcgatcgc cccgaggtag agcggggtgg cgtggcggaa
     2281 cgcgcggcgg cgaaggcgct gccacgggcc gggggtgtag ccgatccctt gctcgaactt
     2341 cgggcggccg cccccgatga ggtagtagcc gacgtgggcc tcgcgcgcct cgcgatcgcg
     2401 ctcggccacc tgccgcgcgc gctcgaccgc cttgagcgcc acccgcacct gcccctcgcc
     2461 ggtcggctcg gccagctctt ccacggcgtg gcggtagcgg tcgcggctgc ggaagtccat
     2521 ccgcccgtag acggccgacg ggtcgcggag cagcacctgc tcgaccaggc tgacgctctc
     2581 gaagaattcg ctccaatcga aggtggagac caggcgcagg ctctcgacga ggttggccat
     2641 cgcggcctgg ctggcggcct ggtactgcgc ctcggcgcgg atcacctcct cgatcgtctg
     2701 gccgcgcgcg tccagctcgg cccccagctc ctggcggagg gcggcggccg ccgccccgtg
     2761 ctcgcgcgac cgctgcagca cgcggatgac gaaggccggg tggaacgggt cgggccaggc
     2821 ccgcggctcc gctcgggggg agtcgagggt cccggccagc cggtcggcgt cgaggcgctg
     2881 cgaccgggcc gcgaagatga cctcggcccg ggcgcgcagc tgctcgacca gcgcgagctt
     2941 gagcgcgctc ggccacgccc agatctcgcc gatcgtcagc ggcgtcaccg actggaacgc
     3001 ggtcatgaag cgctggatgc ggtgcgcgtc gaggcggccg gcgctcgagc ggatcagctc
     3061 gcgcgccatg gccgacacgc gcggcaggcc ggcgaactcg tcggcggtga gcgacggcag
     3121 gcggcggtag aacgagggcg gcaggtcgct gcggacgtcc ctgatggcgg ccgcgatcac
     3181 gtggaagttg tcgagcagcc actcggcggc gggcggcgtc gcctcgccag cgcgcgcgtc
     3241 ctcctcgagg gcggtgtaga tgcggcgcag cgcgcgcgcg tcctctccca gccgcttcaa
     3301 gtgctcgggt cggcccgacc ggcggcggcg ctcgggcgtc agcagcgcgg ccagccgccg
     3361 ggcatgctcc tcgagccgct cgaggccaag cagttcggcg ccctcgcccg actcgcgggc
     3421 gccgtcgaac ccgtcgaatg ccatttccag caattctctc acgaagaccg cacccgcggc
     3481 ctccgaggca agcgcgcacg ccggcgccag ctggtcgccc gacggccgcc acgtcgtctt
     3541 ccagcagaac gcgtcgatct ggatgatgcc gttcgacccc gccgccggcc ggccagccga
     3601 tcctccgcgc cctccagccg atcggtcttg tagtcgagga tgcgccagcc atcggccgcc
     3661 cggtacacga ggtcgatgac gccgcgcagg accgccggtc tctcgaccac ggcaacaccc
     3721 gggatcgacg tgccagcctc caccctcacc gcaaacggca cctcggcgtg cacctcgcta
     3781 cgggccctcg ccttggccca gaacggcgcc cgcgtaacgg cctcgaccag ttcgaccgcc
     3841 tcgggcacca cggcccgcaa cgcgggctgc tccagcgtga gccagcgcgc caggcgctcg
     3901 cccagcgtga ggccgatggc gtgcgcgggc acgacggcca atttcgcgcc ggtggggttg
     3961 gcgcggcaca ggcccgcgag ctgcgagcgg agcggcgctc cacggacggg ccggtgcggg
     4021 cgcgtcgggt ccgggtcgcg gctgccgcgg ttgttcgcgc gcgcggcggg tgtaagctgt
     4081 cgtgcgggag gccatcatgt ttcctcttgc cgccgttctt ctcctcgttg tcatggaagt
     4141 gccggcgctg atatggggcc agcagccggc gccgcccacg ggccagcagt gcgtggattg
     4201 ccacctcaag aacagcccgc agctcgtcgc cgactggcgc gcgagcaagc acttcgaggc
     4261 cgggattggc tgcgagacgt gccatcctgc cgagcacatc gccgacgccg acaagggcgt
     4321 gctcgtcaag cgcggcaacg ccctgccgac gccagagctc tgcgcccagt gccacgctga
     4381 tcgcgtcgac cagttcaaga agggcaagca cgcgatggcc tggacggtga tgaaggcgat
     4441 gcccaccgcg cacatgctgc ccgccacgct gtcggacggc atgaagggct gcggcggctg
     4501 ccacaagatc gggaccaaga ccgacgccga gattcgcgag ctgcggaaga ccggtgtcgg
     4561 ctacggcgtc gcctcgtgcg atgcgtgcca cacgcgccat accttctcgg tggccgaggc
     4621 gcggcagccc gaggcgtgca agacgtgcca catgggcttc gatcacgcgc agtgggagat
     4681 gtactcctcg tcgaagcacg gcgtgcgcca gcagctcagg cagatgaagg tgctgccgga
     4741 ggaggcggcc gcgcccacgt gccagacctg ccacatgccc ggcggcgacc atggggtccg
     4801 caccgcatgg ggcttcctgg ccgtgcggct gccgatgccc gaggacaagc agtgggccga
     4861 ggatcggacc gtgatcctgc aggcgctcgg cgtgctcgac gcgtcgggga agccgacggc
     4921 gcggctcgat acggtgaagg cggccgacct cgccaggctg acgcaggaga gctggcagca
     4981 ggagcgcgac gccatgcaga aggcgtgcgc ctcgtgtcac gtgcagtcgt ttgcgaaggc
     5041 cgagctcgcc aagggcgacg acatgatccg gcaggccgat cgcctgatgg ccgaggcgat
     5101 ccgtaccgtc gccgcgctat acgcggacgg cgtcctgaag aagccgtcgg cggccgactc
     5161 gccgtatccg gacctgctcg agttccacga cgcgtcgtcg cagatcgaac agaagctctt
     5221 cgtcatgttc ctcgagcacc ggatgcgcgc cttccaggga acgttccacg cgagcccgga
     5281 ttacgcgctc tggtacggct ggagcgagct gaagcgcgac gtcccgatgc cgccgggagc
     5341 ggagctactg cttcacggcg acggccgcat cgggacccgg cgccggcgcg gtgaggtcgt
     5401 ccgcgccggc gtcgatcgac tggtgctccg ccagcggcct gggg
//
LOCUS       DAOSIF010000170         5435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13487_length_5435_cov_3.389219, whole genome shotgun sequence.
ACCESSION   DAOSIF010000170 DAOSIF010000000
VERSION     DAOSIF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13487_length_5435_cov_3.389219"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLT35_07005"
     CDS             <1..199
                     /locus_tag="PLT35_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOG29013.1"
                     /translation="LAPGAHAALAGADALALVTEWHEFLEPDFGKMRKLMKSPVVFDG
                     RNVYSRDTMRAHGFTYYGIGR"
     gene            183..1199
                     /gene="galE"
                     /locus_tag="PLT35_07010"
     CDS             183..1199
                     /gene="galE"
                     /locus_tag="PLT35_07010"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOG29014.1"
                     /translation="MASDAEREAVLVTGGAGYIGSHAARALVGAGRRAVVVDNLSMGH
                     REAVRWCDLVAADLHDEDAMRRTIRQYGVTAVMHFAALASVGLSVADPAAYYRENVEG
                     TLSLLRAMRAEGVSRLVFSSTAAVFGEPIDTPITEDHPTRPINPYGETKLVIERALAH
                     YERAYGLHSVTLRYFNAAGADPEGILGEDHRPEEHVLPIAIDAALGRGQLEVFGGDYP
                     TPDGTCLRDYVHVTDLAQAHLLALARLESGAGSAVYNLGNGRPFSVRDVIRAVERVTG
                     RTVPWSLGGRRPGDPAVLFASSARIRSDLGWSPAISSLEDIVDTAYRWRLAHPAGYGG
                     EDSR"
     gene            1196..1909
                     /locus_tag="PLT35_07015"
     CDS             1196..1909
                     /locus_tag="PLT35_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG29015.1"
                     /translation="MKARLRGALEDPLLSVVIPVYNERETIEEILTRVRAVPIRTEVI
                     VVDDGSTDGTRDLLARLQPRLGFRLFLQDRNRGKGSALRRGFAEATGDLVIIQDADLE
                     YSPEEYPALIELICQGRADVVYGSRFLGRHRVFLFTHYLGNRFLTLVTNVLYNTMLTD
                     METCYKVMRMEVVRSFALRSNGFGIEPELTAKIFKRGFRVYEVPITYDGRGYDEGKKI
                     TWRDGVVALWVLLRYRFSE"
     gene            1906..3675
                     /locus_tag="PLT35_07020"
     CDS             1906..3675
                     /locus_tag="PLT35_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOG29016.1"
                     /translation="MSPLRRLLRYAAPYRGRFAVALAAMAAYGGASAWLAYLVKPIFD
                     EVLIKQQSLSTVAWAILAAYLLKGLGSYASSYLMADVGQHVVLDLRNEMFRRTLGQSA
                     AFFKSRSTGQLLSRLTNDIAQVQQVVSETVADLLRESMAVVGFAGLLFSYDAGLALVC
                     LTGAPLVMYPLVKLGQRLRRTTKRSQEHLEHMSHVATEAFTGHRIVKAFGAEEREAAR
                     FGLAARALYRVNLAVTATVSVMPPVMEFLGGVAMATALWYGSREIGAGRLTEGEFTAF
                     LAALFLMYGPLKKLSRVNANIQQSIAASQRIFELIDTRSEVPEAPGAPALPPLARAIE
                     FRDVSFEYEDARGKSALRHISFTVRAGQTAAIVGRSGAGKTSLVNLIPRFYDVTGGAI
                     LIDGTDITRVSLASLRGQIGMVTQDTVLFDDTVAANIAYGRPAATAAAIEAAARAARA
                     HEFIAGLPQGYRTVIGERGQRLSGGQRQRLAIARAILSDAPILILDEATSALDSESEG
                     LVQDALANLMKDRTSFVIAHRLSTIQRADVIIVLERGRVAEVGRHEELLANPDGIYAA
                     LHAVQFAAPRRRAATAPAGKAGA"
     gene            3672..4565
                     /locus_tag="PLT35_07025"
     CDS             3672..4565
                     /locus_tag="PLT35_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOG29017.1"
                     /translation="MIKSMTGFASMTREDDRASIGVTVRSVNHRYLDLQLRVPQTLGH
                     LEPRLRAAIQPRVARGRVELAVAVQLRQEPALRVEINEAFTAALADAMARARQAGLVS
                     GDLLPGDLLRFPQALTVHEEAPDLDEQGRAALEGAVLEAVDAALAELDAMRRREGALL
                     QADLDARKRSVLEAVERIAVKAAEAEAGLRERLAARVADLAGDLSLDSSAIAQEVVRF
                     AARSDISEELVRFRAHVQQWSALTDGPEACGRKLDFLLQEMNREVNTMGSKADGAAVP
                     EAIIDVKAELEKMREQVQNVE"
     assembly_gap    4697..4706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4707..5195
                     /locus_tag="PLT35_07030"
     CDS             <4707..5195
                     /locus_tag="PLT35_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOG29018.1"
                     /translation="DGVDYRFVSRGRFEAMAGAGEFLEWADVFGDLYGTHAGDTERAL
                     SEGLDLVLVIDVQGARQVRARRVPSVSIFVLPPSFAELEARLRARGEDTEEQIRRRLD
                     VARREVLAVPEYDYVVVNDDVDGCADRVRAIVLAERSRRDRIAATADAIARTFERADG
                     AR"
     gene            5223..>5435
                     /locus_tag="PLT35_07035"
     CDS             5223..>5435
                     /locus_tag="PLT35_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOG29019.1"
                     /translation="MARIALGISGGIGAYKAVEVARGLQQRGHDVVAVMTRNARRFIG
                     RLTLEAITRHPVVTSQWTNGANAGVEH"
BASE COUNT          765 a         1852 c         2029 g          779 t
ORIGIN      
        1 gctggcgccg ggcgcccacg cggcgctggc gggcgcggac gcgctggccc tggtcacgga
       61 gtggcacgag ttcctcgagc cggacttcgg caagatgcgc aagctcatga agagcccggt
      121 cgtgttcgat ggcaggaacg tgtattcccg cgacacgatg cgggcgcacg ggttcaccta
      181 ctatggcatc ggacgctgag cgcgaagcgg tcctcgtcac cggcggcgcc ggctacatcg
      241 gcagccacgc ggccagggcc ctcgtgggcg cggggcggcg ggccgtcgtc gtggacaacc
      301 tctcgatggg gcaccgcgaa gccgtgcggt ggtgtgatct cgtcgccgcc gaccttcacg
      361 acgaggacgc gatgcgccgg acgatcaggc agtacggcgt caccgcggtg atgcacttcg
      421 ccgccctggc gtcggtcggc ctttcggttg ccgacccggc cgcgtactac cgcgagaacg
      481 tcgagggcac gctgagcctg ctccgcgcca tgcgggccga gggcgtgagc cgcctcgtgt
      541 tttcctcgac ggccgccgtg ttcggggagc cgatcgacac gcccatcacc gaggaccacc
      601 ccacgcggcc catcaacccg tacggcgaga ccaagctggt catcgagcgc gcgctcgcgc
      661 actacgagcg cgcgtacggg ctccactcgg tgaccctgcg ctatttcaac gccgcggggg
      721 ccgatcccga ggggatcctg ggggaggatc accgccccga ggagcacgtg ctgccgattg
      781 ccatcgacgc ggcgctcggg cgggggcagc tcgaggtgtt cggcggcgac tacccgacgc
      841 cggacggcac ctgcctccgc gactacgtcc acgtcaccga tctcgcccag gcgcacctgc
      901 tggcgctcgc ccgcctggag tccggcgcag ggtcggccgt ctacaacctc gggaacggtc
      961 ggccgttctc ggttcgggac gtcatccgcg ccgtcgagcg cgtgaccggg cggacggtgc
     1021 cgtggtcgct cgggggccgg cggccgggcg atcccgcggt tctattcgcc tcgagcgcgc
     1081 ggatccggag cgatctcggc tggtcgccgg cgatctcgag cctcgaggac atcgtcgaca
     1141 ccgcgtaccg ctggcgcctg gcgcatccag ccggctacgg cggggaggac agccggtgaa
     1201 ggcgcgtctg cgcggggcgc tggaggaccc gctgctctcg gtcgtcatcc ccgtctacaa
     1261 cgagagggag acgatcgagg agatcctgac gcgcgtgcgc gcggtgccca tccgcaccga
     1321 ggtgatcgtc gtcgacgacg gctccaccga cggcacgcgg gacctgctgg cccgactgca
     1381 gccgcgcctc gggttccggc tgttcctcca ggaccggaac cggggcaagg gctcggcgct
     1441 tcgccggggc ttcgcggagg cgacggggga cctggtcatc atccaggacg cggacctcga
     1501 gtactcgccc gaggagtacc ccgcgctcat cgagctcatc tgccagggcc gcgccgacgt
     1561 ggtgtacggc tcgcggttcc tgggccgcca ccgcgtcttc ctgttcacgc actacctcgg
     1621 caaccgtttc ctgacgctgg tgacgaacgt gctgtacaac acgatgctga cggacatgga
     1681 gacctgctac aaggtcatgc ggatggaggt cgtgcgctcg ttcgcgctcc ggtccaacgg
     1741 gttcggcatc gagccggagc tgaccgcgaa gatcttcaag cggggattcc gggtgtacga
     1801 ggtgcccatc acgtacgatg ggcggggcta cgacgagggc aagaagatca cctggcgcga
     1861 cggcgtggtg gcgctctggg tgctcctgcg gtaccggttc agcgagtgag cccgctcagg
     1921 cgcctgcttc gctatgccgc gccctaccgg gggcggtttg ccgtcgccct ggcggccatg
     1981 gcggcctacg ggggcgcgtc ggcgtggctg gcctacctgg tcaagccgat cttcgacgaa
     2041 gtcctgatca agcagcagtc gctgtccacc gtcgcgtggg cgatcctcgc cgcgtacctg
     2101 ctgaagggcc tggggtcgta cgcctcgtcg tatctgatgg ccgacgtcgg ccagcacgtc
     2161 gtgctggatc tgcggaacga gatgttccgg cgcacgctgg gccagtcggc ggcgttcttc
     2221 aagtcgcgca gcacgggcca gctcctgtcg cgcctgacga acgacatcgc acaagtgcag
     2281 caggtggtgt cggagaccgt tgcggatctg ctgcgcgagt cgatggcggt cgtcggcttc
     2341 gccggcctgc tgttctcgta cgacgccggc ctggcgctcg tctgcctgac gggcgcgccg
     2401 ctcgtgatgt atcccctcgt caagctgggg cagcggctgc ggcggacgac gaagcggagc
     2461 caggagcacc tcgagcacat gtcgcacgtg gccaccgagg ccttcacggg ccaccgcatc
     2521 gtcaaggcgt tcggagcgga ggaacgggag gcggcgcggt tcgggctggc ggcgcgggcg
     2581 ctctaccggg tcaacctcgc cgtgacggcc acggtgtccg tgatgccgcc ggtgatggag
     2641 ttcctggggg gagtcgcgat ggcgacggcc ctctggtacg gcagccggga gatcggggcg
     2701 ggccgcttga cggaagggga gttcacggcc ttcttggcgg cgttgttcct gatgtacggg
     2761 cccttgaaga agctgagccg cgtcaacgcg aacatccagc aatccatcgc ggcgtcgcag
     2821 cggatcttcg agctgatcga tacgcggtcg gaggttccgg aggcgcccgg cgcgcccgcc
     2881 ctcccgccgc tcgcgcgggc gatcgagttc cgcgacgtca gcttcgagta cgaagacgca
     2941 cgggggaaga gcgcgcttcg gcacatctcg ttcacggtgc gcgcggggca gacggcggcg
     3001 atcgtcggcc ggagcggggc gggcaagacg agcctcgtca acctcatccc gcggttctac
     3061 gacgtgaccg gaggcgcgat cctgatcgac ggcacggaca tcacgcgggt gtcgctcgcg
     3121 tcgctccggg ggcagatcgg catggtgacg caggacaccg tgctgtttga cgacaccgtc
     3181 gccgccaaca tcgcctacgg ccggccggcc gccacggccg cggccatcga ggcggcggcc
     3241 cgggcggccc gggcgcacga gttcatcgcg gggctgccgc agggctaccg gaccgtcatc
     3301 ggagagcgcg gccagcggct ctcgggaggc cagcggcagc ggctcgcgat cgcccgggcg
     3361 atcctgagcg acgcgccaat cctgatcttg gacgaggcga cctcggcgct cgactccgag
     3421 tccgagggcc tcgtgcagga cgccctcgcg aacctgatga aggaccggac gtccttcgtc
     3481 atcgcgcacc ggctctcgac cattcagcgc gcggatgtca tcatcgtgct ggagcgcggc
     3541 cgggtcgccg aggtcgggcg ccacgaggag ctgctggcga acccggacgg catctacgcc
     3601 gccctgcacg cggtgcagtt cgcggcgccg cggcgccggg ccgccacggc cccggcgggg
     3661 aaggcgggcg catgatcaag agcatgaccg ggttcgcctc catgacgcgc gaggacgacc
     3721 gcgcgagcat cggcgtgacg gtccgcagcg tgaaccaccg gtacctcgac ctccagctgc
     3781 gggtgccgca gacgctcggg cacctcgagc cccgcctccg ggcggcgatc cagccgcgcg
     3841 tcgcccgggg ccgcgtcgag ctggcggtgg ccgtgcagct gcgccaggag cccgcgctcc
     3901 gcgtcgagat caacgaggcc ttcacggccg ccctcgccga cgccatggcg cgggcccggc
     3961 aggctggcct cgtctcggga gacctcctgc cgggcgacct cctccggttc ccgcaggccc
     4021 tgaccgtgca cgaagaggcc ccggacctgg acgagcaggg ccgagccgcc ctcgagggcg
     4081 ccgtcctgga ggcggtggac gccgcgctcg ccgagctcga cgccatgagg cggcgggaag
     4141 gggcgctcct ccaggccgac ctggacgcgc ggaagcggag cgtgctcgag gcggtggaac
     4201 gcatcgcggt caaggcggcg gaggccgagg ccggcctgcg ggagcgcctc gcggcgcgcg
     4261 tcgccgacct ggcgggcgat ctctccctcg actcgtccgc catcgcccag gaggtggtcc
     4321 ggttcgcggc tcggtcggac atcagcgagg agctggtgcg cttccgcgcc catgtgcagc
     4381 aatggtctgc gctcaccgac ggcccggagg cgtgcggccg caagctggat ttcctgctgc
     4441 aggagatgaa ccgcgaagtc aacaccatgg gctcgaaggc ggacggcgcg gccgtgccgg
     4501 aggccatcat cgacgtcaag gccgaactcg aaaagatgcg ggagcaggtg cagaatgtcg
     4561 aatgaggcgg ccggcggcgg gcggctcatc gtggtgtcgg ccccttcggg ggcgggtaag
     4621 acgaccatcg tggagcgcgt cgtgcgggcc gtgcctgggc tgcgcaggtc gcgctcgtac
     4681 acgagccggc ccgcgcnnnn nnnnnngacg gcgtcgacta ccgcttcgtc agccgcgggc
     4741 ggttcgaggc gatggccggc gccggcgagt tcctggagtg ggccgacgtc ttcggcgacc
     4801 tgtacggcac ccatgccggg gacaccgagc gcgccctgtc ggagggcctc gatctcgtgc
     4861 tggtgatcga cgtccagggg gcccgccagg tgcgggcgcg ccgggtcccg tcggtctcga
     4921 tcttcgtgct gccgccgtcg ttcgccgaac tcgaggcgcg gctgcgcgcg cgcggcgagg
     4981 acaccgagga gcagatccgg cgccgcttgg acgtggcgcg gcgcgaagtg ctcgccgtgc
     5041 cggagtacga ctacgtcgtt gtcaacgacg acgtggacgg gtgcgcggat cgcgtgcggg
     5101 cgatcgtgct ggccgagcgg tcgcggcgcg accggatcgc cgcgacggcg gacgccatcg
     5161 cgaggacatt cgaacgagcg gacggggcgc gctgagggcg cagcccgagg gagcagggcg
     5221 ctgtggccag gatagcactc gggatcagcg gcggcatcgg ggcgtacaag gccgtcgagg
     5281 tggcgcgggg cctccagcag cggggccacg acgtcgtcgc ggtaatgacg cgcaacgcgc
     5341 gccggttcat cggtcgcctc accctcgagg ccatcacccg ccatccggtc gtgacgagcc
     5401 agtggacgaa cggcgccaac gccggcgtcg agcac
//
LOCUS       DAOSIF010000171         5417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13532_length_5417_cov_4.374487, whole genome shotgun sequence.
ACCESSION   DAOSIF010000171 DAOSIF010000000
VERSION     DAOSIF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13532_length_5417_cov_4.374487"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="PLT35_07040"
     CDS             <1..1835
                     /locus_tag="PLT35_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29020.1"
                     /translation="YGPQQDNTTVVVPSLPPVAWGLDSPVQAWGQVAGCETGQVWPRP
                     DGSVVWGACKGQVGRYVTATGQEQQYWVYPQNRYGHNPKDIKYRFPRQTVVYLSPHDP
                     KTIYQASHVVHRSVDEGVTWETISHDLTAYEPDKQITPGTPITRDITGEEVYSSLYAF
                     VESRLEPGVLWAGSNDGPVHVSRDAGKSWRRVTPPDLPPGGRVQTIEDSPHRKGSAYI
                     AVYRYLREHDLEPYIYATADYGATWTRLTDGRNGIPGDHPTRVVREDPSREGLLYAGT
                     EFGLFVSFDNGRHWQPLQQNLPHTPVTDIRVHRQDLVLSTMGRGFWILDDVTLLQQLA
                     ARHELVMHSEGYLFQPRDAWRMRYRPTGGSAGQPEYPPPGAIIDYYLAGEPAGEVKLE
                     IMDGKRGVIRTLSVGGGAGGAAGAPGQAAGQQTGPAAPPRRGRAGAALPRRAWHNRFV
                     WDLRLEGSGGSPGPLAVPGSYLLRLSVGEWSQTRTIEVRIDPRVAAAGVTQADLQEQL
                     DLALAIRDAMSGASALASKIAAAGQAATANAASARALQSLHGRLVTAGGAYPQPMLID
                     QLANVALMISQADQKVGRDAFARFEDLSKELAAVEAEAARLGIR"
     gene            1886..4243
                     /locus_tag="PLT35_07045"
     CDS             1886..4243
                     /locus_tag="PLT35_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG29021.1"
                     /translation="MAAIRTGRGAAVAVVCLAGATAAGLWCGGPPPAQPATAPDASFS
                     AARAARLVERIAERPHHAGSADHGRVRDLLIAEFAALGAAVERQAATVLVGTVEARAA
                     RVENLVFRLPGTASTGAVLLVAHYDSVAAGPGAADDGAGVAAIAETVRALRAGPPLAN
                     DVIALVSDAEEFGMLGARAFATGHPRAKDVRMVLNLEARGTSGPLQLFETSAGNGSVV
                     AAWAADAPRPTGSSLAYEVYRRLPNDTDFSVLKKLDTAGLNLAFIGNPERYHTPQDTP
                     AALDLRSLQHMGDTTLALARRFGGMDLGRLRARDAVFFSLPVPGMVVRYSSLLALPLA
                     AAACGLMAIAFLRARRRRETSIPGALVAAVLFAAFAAASTAAGWRFGRWSHALHARWM
                     GGGSVVWIAAWAGALVGSIATAWCALYAALRKVFAAHSIAFGAMLVLACAELALAWFV
                     VGASYVLVWPLAGAALAAMALRAAWDIPPGAGPARALVITAFGLPAVLVLWPLVGMLF
                     TAMGLSPQGGAAMGAVAACALGALAVPLEVVAQGRRWWPAALALAATIASLAAGMAGS
                     RYSERFPRAVNAYYALHADTRAAHWAVQSVSAGRPEPSGRDGWPDQWARQYVGDEPHP
                     GRPYSLVPPWSTLEGTPGFLTAGAPLLDLPAPRASLLSSARSEGGRSVRIRVFPAREG
                     RVLSVWVHGSQVLDAAVDDLRIAGPMPARRENDTAWSLDYVNAPAPGIVLTLVLRDAR
                     PLTVAVLDRTAGLPAVPGRAFAPRPATVTPIQFGDQTVVRRTYVF"
     gene            complement(4326..4898)
                     /locus_tag="PLT35_07050"
     CDS             complement(4326..4898)
                     /locus_tag="PLT35_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29022.1"
                     /translation="MDLGPVERSLKELTLAPYIVKAMAMIDRPRKAGSNMFRHQMSTM
                     AILLDYKIIDPILLKASVVHDLYEELGHEPWVNRDTIATIDADGPAVYDLVAEVTIRE
                     TNGVREPKSDYLLRIMQTGSFRAKQLKLADRISNLVGLGFVHDRAFVERYTSQTRTCI
                     LPYAEAVNADMYRELRDLVNDRTAHLAHLL"
     gene            complement(4952..>5417)
                     /locus_tag="PLT35_07055"
     CDS             complement(4952..>5417)
                     /locus_tag="PLT35_07055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOG29023.1"
                     /translation="QVQFMPSSYLAYAADGDGDGRRDIWRSLPDVFASIANYLKAHGW
                     VPGTRWGREVRLADAVAARATAEVPLRLASPCRALRDMSEARPLSAWRSLGVTQRSGA
                     QLPRSELPASLVRVDARAFLVYANYEALLAYNCANTYALSVAMLADRIGGAW"
BASE COUNT          694 a         1925 c         2042 g          756 t
ORIGIN      
        1 tgtacggccc gcagcaggac aacacgaccg tcgtggtgcc gagcctgccg cccgtggcct
       61 ggggcctcga ctcgccggtc caggcctggg gccaggtggc cggctgcgag acgggccagg
      121 tctggcccag gcccgacggg tcggtcgtct ggggcgcctg caaggggcag gtcggccgct
      181 acgtgacggc cacggggcag gagcagcagt actgggtcta cccgcagaac cgctacggac
      241 acaacccgaa ggacatcaag taccggttcc cgcggcagac ggtcgtctac ctctcgccgc
      301 acgatccgaa gacgatctac caggcctcgc acgtcgtgca ccgctccgtg gacgagggcg
      361 tgacgtggga gaccatcagc cacgacctga cggcctacga gccggacaag cagatcacgc
      421 cgggcacgcc gatcacgcgc gacatcacgg gcgaggaggt ctacagcagc ctctacgcct
      481 tcgtcgaatc gcgtctcgag ccgggcgtcc tgtgggccgg ctcgaacgac gggcccgtgc
      541 acgtctcgcg cgacgccggg aagagctggc gccgcgtcac gccgccggac ctgccgccgg
      601 gcgggcgcgt gcagacgatc gaggactcgc cgcaccgcaa gggctcggcc tacatcgccg
      661 tctaccgcta cctgcgcgag cacgatctgg agccgtacat ctacgccacg gccgactacg
      721 gcgccacctg gacgcgcctg acggacggcc ggaacggcat tcccggagac catccgacgc
      781 gcgtcgtgcg cgaggatccg tcgagggagg gcctgctcta cgcgggaacc gagttcggcc
      841 tgttcgtgtc gttcgacaac ggccgccact ggcagccgct gcagcagaac ctcccgcaca
      901 cgccggtgac cgacatccgc gtccaccgcc aggatctcgt gctctcgacg atgggcaggg
      961 ggttctggat cctggatgac gtgacgctgc tccagcagct ggcggcgcgg cacgagctgg
     1021 tcatgcactc ggagggctac ctcttccagc cgcgcgacgc ctggcggatg cgctaccggc
     1081 cgacgggcgg atcggccggt cagcccgagt acccgccgcc gggcgcgatc atcgactact
     1141 acctcgcagg agagccggcg ggggaggtga agctcgagat catggacggc aagcgcggag
     1201 tgatccggac gctgtcggtc ggcggcggag cgggcggcgc ggccggagcg cccggccagg
     1261 cggcgggcca gcagacgggc ccggcggcgc cgccgcggcg cgggcgcgca ggcgccgcgc
     1321 tgcccagacg ggcgtggcac aaccggttcg tctgggacct caggctggag gggagcggag
     1381 gctcgcccgg cccgctcgcg gttcccggct cgtacctgct gcgcctgtct gtgggcgagt
     1441 ggagccaaac gcgcacgatc gaggtgcgca tcgacccgcg cgtcgcggcg gcgggcgtga
     1501 cgcaggcgga cctgcaggag cagctcgatc tcgcgctggc gatccgcgac gcgatgtcgg
     1561 gcgcatcggc gctggcctcg aagatcgccg ccgccgggca ggcggccacg gcgaacgccg
     1621 cctcggcgcg ggcgctgcag tcgctccacg gccgcctcgt cacggccggc ggcgcgtacc
     1681 cgcagccgat gctcatcgac cagctggcca acgtggcgct gatgatctcc caggccgatc
     1741 agaaggtcgg ccgcgacgcg ttcgcgcggt tcgaggacct gtcgaaggag ctggccgccg
     1801 tcgaagccga ggccgccagg ctcgggatcc gctgaaggag ggcggttcgc agaagcagcg
     1861 gggctgctga cgaaaggagg cgggcgtggc cgcgatccgc acgggaagag gagcggcggt
     1921 ggcggttgtg tgcctcgccg gcgcaacggc cgccggcctg tggtgtggcg ggccgccgcc
     1981 ggcgcagccg gccacggcgc cggacgcctc gttctcggcc gcccgggccg cccgtctcgt
     2041 cgagcgaatc gccgaacgcc cccaccacgc cggatcggcg gaccacggcc gcgtgcgcga
     2101 tctgctcatc gccgagttcg ccgcgctggg cgcggccgtc gagcggcagg ctgcgacggt
     2161 ccttgtgggc accgtcgagg cccgcgccgc gcgcgtcgag aacctggtct tccgcctgcc
     2221 gggcacggcc agcaccggcg ccgtcctgct cgtcgcgcac tacgactcgg tcgccgccgg
     2281 gcccggggcc gccgacgatg gcgccggggt ggcggcgatt gccgagacgg tgcgggcgct
     2341 tcgggccggc ccgcccctgg cgaacgacgt catcgcgctc gtgagcgacg ccgaggagtt
     2401 cggcatgctc ggcgcgcgcg cgttcgccac ggggcaccct cgggcgaaag acgtgcggat
     2461 ggtcctgaac ctcgaggccc gggggacgag cgggcccctc cagttgttcg agacgagcgc
     2521 gggaaacggc agcgtcgtgg ccgcctgggc ggcggacgcc ccgaggccga ccggatcgtc
     2581 gctggcgtac gaggtctaca ggcggcttcc caacgacacg gacttctccg tgctcaagaa
     2641 gctcgacacg gcggggttga acctcgcctt catcgggaac cccgagcgct accacacccc
     2701 gcaggacacg cccgccgcgc tcgacctccg cagcctgcag cacatgggag acacgacgct
     2761 cgcgctggcg cgccggttcg gcggcatgga tctcggccgg ctgcgggccc gcgacgcggt
     2821 gtttttctcg ctccccgtcc cgggcatggt ggtgcgctac tccagcctgc tggccctgcc
     2881 gctggcggcg gcggcgtgcg gcctcatggc gattgccttc ctccgggcgc ggcgccggcg
     2941 cgagacgagc attcccgggg cgcttgtggc ggcggtcctg ttcgccgcct tcgcggcggc
     3001 ctcgaccgcc gccggctgga ggttcggccg ctggtcgcac gcgctgcacg cccgctggat
     3061 gggcggcggc agcgtggtct ggatcgcggc gtgggcgggc gccctcgtgg gctcgatcgc
     3121 gacggcctgg tgcgcgctgt acgccgccct ccggaaggtg tttgccgcgc actcgatcgc
     3181 gttcggcgcg atgctggtgc tcgcctgcgc cgaactggcc ctcgcatggt tcgtcgtcgg
     3241 cgccagctac gtgctcgtgt ggccccttgc gggcgccgcg ctggcggcca tggcccttcg
     3301 ggccgcgtgg gacatcccgc ccggggccgg cccggcgcgg gcgcttgtca tcacggcctt
     3361 cggcctgccg gcggtcctcg tcctgtggcc tctcgtcggg atgctcttca cggcgatggg
     3421 cctgtcgccg cagggcggcg cggccatggg cgccgtcgcg gcgtgcgcgc tcggcgcgct
     3481 cgcggtcccg ctcgaggtcg tcgcccaggg gcggcggtgg tggccggccg cgctggcgct
     3541 ggccgcgacg atcgccagcc tggcggcggg catggcgggc agccgctact cggagcgttt
     3601 cccgagggcc gtcaacgcgt actacgcgct gcacgccgac acgcgggccg cgcactgggc
     3661 ggtgcagtcc gtgtcggcgg gccggcccga gccgtcgggc cgggacggct ggccggacca
     3721 gtgggcgagg cagtatgtgg gggacgagcc gcacccgggc aggccctact cgctcgtgcc
     3781 gccctggtcc accctggagg gcacgcccgg cttcctcacg gccggggcgc cgctcctcga
     3841 tcttccggcg ccgcgcgcct cgctcctgtc gagcgcgcgc tccgaggggg gccggagcgt
     3901 gaggattcgc gtgttccccg cccgcgaagg gcgcgtgctc tccgtctggg tgcacggttc
     3961 gcaggtgctc gacgcagccg tggacgacct gcggatcgcg gggccgatgc cggccaggcg
     4021 cgagaacgac acggcgtgga gcctggacta cgtcaacgcg cccgcgccgg gaatcgtcct
     4081 taccctcgtc ctcagggacg cccggcccct caccgtcgcc gtgctggatc gcacggccgg
     4141 cctgcccgcc gtccccggcc gcgcgttcgc gccgcgcccc gcgacggtga cgcccatcca
     4201 gttcggcgac cagaccgtcg tgcggcgcac gtacgtgttc tgaccgcggc gcgccgagtg
     4261 cgccgggccc agggttggct ggggggcgcg cgcgagcgtg agcgtgaggg cgcgaacggg
     4321 cctggctaga gcaggtgggc gaggtgggcg gtgcggtcgt tgacgaggtc cctcagttcg
     4381 cgatacatgt ccgcgttcac cgcctcggcg tacggaagga tgcacgtgcg cgtctgcgag
     4441 gtgtagcgct cgacgaacgc gcggtcgtgc acgaagccca ggccgacgag gttgctgatg
     4501 cggtcggcga gcttcagctg cttcgcccgg aagctccccg tctgcatgat gcgcagcagg
     4561 tagtcgctct tcggctcgcg gacgccgttc gtctcgcgga tcgtcacttc cgcgacgaga
     4621 tcgtagacgg ccggcccgtc ggcgtcgatt gtggcgatgg tgtcccggtt cacccacggc
     4681 tcgtggccga gctcctcgta caggtcgtgc acgacggacg ccttcaggag gatggggtcg
     4741 atgatcttgt agtcgaggag gatggccatc gtgctcatct ggtggcggaa catgttgctg
     4801 cccgccttgc ggggccggtc gatcatggcc atcgccttca cgatgtaggg tgcgagggtc
     4861 agctccttga ggctgcgttc aacgggtccg agatccatcg ggcgtcactc ttccttggcg
     4921 caagagtcta ctgcaaaacc ggcccggatc cctaccaggc gcccccgatc ctgtcggcga
     4981 gcatggcgac gctcagcgcg taggtattgg cgcagttgta ggcgagcagc gcctcgtagt
     5041 tcgcgtacac caggaaggcg cgggcgtcga cgcgcacgag ggaggcgggc agctcgctcc
     5101 ggggcagctg ggcgccgctg cgctgggtca cgcccagcga gcgccaggcc gacagcggac
     5161 gcgcctcgct catgtcgcgc agggcgcggc acggagaggc caggcgcaag ggcacctcgg
     5221 ccgtcgcgcg ggccgccacg gcgtcggcga gccgcacctc gcgcccccag cgcgtccccg
     5281 gcacccagcc gtgggccttc agatagttgg cgatcgacgc gaagacatcg ggcaacgagc
     5341 gccagatgtc acgccggccg tcgccgtcgc cgtctgcggc ataggccagg tagctcgagg
     5401 gcatgaactg gacctgg
//
LOCUS       DAOSIF010000172         5397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13587_length_5397_cov_3.928304, whole genome shotgun sequence.
ACCESSION   DAOSIF010000172 DAOSIF010000000
VERSION     DAOSIF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13587_length_5397_cov_3.928304"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="PLT35_07060"
     CDS             <1..1385
                     /locus_tag="PLT35_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG29024.1"
                     /translation="NQVVMITGHLYEGYTKQGANDNVSGCALSLEMGRAYLRLVADGA
                     LPKPKRAIHFLWVPEISGTMAWLRAHDDVAKRIVANLNFDMEGLLLRLSGSAWVMHRT
                     PDSVPSFLNDLCASVLRFVGETNRERVRYRSVAYGFTLPVVSQNGSRDPFFVIEDPYY
                     GASDHVVFINQGIPAVMFITWPDNFYHSSQDTPERLDPTQMKRAAVVGIGCMSLVASA
                     GDETGLKVAAESLARGAERMGQAQRKGASYIADLSFGLSLPEAYKEARTAIRHQANVE
                     KAVVKSASVLFADPAAAAKKLAAFDLLIDQRAASLQNEIAALCRLRAEQLKLPWAEPA
                     PTDLEKRAAATLVEALPQPAGASPFGGGGRRAQTGPMTPAMQAMADARRKIPAHILGE
                     LGTVMRQGRTVLEIRDFISGEFEPVPLQDVFDYLKAAEALGQVRLVEKPAAPATKKAP
                     ATVKAPAQAG"
     gene            1391..1933
                     /locus_tag="PLT35_07065"
     CDS             1391..1933
                     /locus_tag="PLT35_07065"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOG29025.1"
                     /translation="MARQHEHVTYFTYLDTPVGVLMLAGCPDHGLRHVAFQCGQGALA
                     PEPDWKPSKKPFREAERQLIEYFHGRRTRFDLRLSPTGTAFQTAVWKALQRIPYGETR
                     SYGEIAKAVGRPRAARAVGLANARNPLPIVVPCHRVIGSTGRLVGFGGGLPVKQALLD
                     LERRVSRARRTRPAPRRSSS"
     gene            complement(1884..3473)
                     /locus_tag="PLT35_07070"
     CDS             complement(1884..3473)
                     /locus_tag="PLT35_07070"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG29026.1"
                     /translation="MLNGRVWTGLAGRSEAGAVAVAGDRIAAVGPTSALAGWRGRATT
                     VIDARGARIVPGFNDSHVHLMAGGRQLDSVDLREAASPAELARLVGERAAVTPAGSWV
                     LGGGWDEQRWDRALPPDRDLLDRAAPATPVFVARYDLHMAAANSLALELAGIAAGTPD
                     PPGGVIVRDRGGRATGVLKDAAMALVVRAVPPPTRAQRTATLRRALDHAASLGVTSVR
                     DMGPEPDDLAALAELADAGRLTLRVSAAPPAARWEEQANWGARSAFGTPWLSLGAVKA
                     FADGSLGSSTALFFEPYADAPDTIGLLADDLTPLEDARRRLIAADAAGLQLCVHAIGD
                     RAVSIVLDVFTDVVAANGPRDRRLRIEHAQHVAEADFDRFASLGVIAAVQPYHAIDDG
                     RWAERRIGPRRIRSAYPFRTLLEKGVRLALGSDWPVAPLNPLLGIYAAATRATLDGAH
                     PEGWVPRQKITVEDALRGYTSGSAYAEFTDGRKGVVAPGAFADLAILSDDILAIAPDR
                     VREARVTTTIVGGRVVFDARG"
     gene            3656..4129
                     /locus_tag="PLT35_07075"
     CDS             3656..4129
                     /locus_tag="PLT35_07075"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG29027.1"
                     /translation="MAKARWDGRFRMEDAGQKAYNVQQFQDLVKDIPFAMFTTMNGER
                     GLRSRPMVASAGAFDGDLWFFTRTSSAVAQELAKNAVVNITYVSAPEDRFISVSGNAC
                     VVRDVEHALAMWHPAYNQWFPGGAADADLSLIKVEVRRVEYWDRKAGRMKDLEAR"
     gene            4256..4726
                     /locus_tag="PLT35_07080"
     CDS             4256..4726
                     /locus_tag="PLT35_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG29028.1"
                     /translation="MMKRAVFVMVAVLLALALGADAGQGTGRISGTARDAQNRVLANT
                     TVQLRHVDTGQLVATTRTAGDGRFEFTGLNPGNYVVEIVDTDGKIIGLSPSTALAAGG
                     FVSGLAVAASAAGAAAGAGTAFFASTGGILVLAGAGIALTAGVIALTQDASASK"
     gene            complement(4845..>5397)
                     /locus_tag="PLT35_07085"
     CDS             complement(4845..>5397)
                     /locus_tag="PLT35_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG29029.1"
                     /translation="TVLYARTAPGQLAAGRAELEFLLRRMRGLPPGADNDFTLSTAEQ
                     IISNLDAISAQIGLAAFALALVSLVIGGIGIANVMIIAVTERTREIGTRLAIGAKRRD
                     VLAQFLVEAALLSAVGGGVGVAVSWALGFALSLAAPAFPAVPPLWAVAGGLGSAVGTG
                     LVAGYLPARRAANLDPVEALRYE"
BASE COUNT          825 a         1912 c         1935 g          725 t
ORIGIN      
        1 cgaaccaggt cgtgatgatc accggccacc tctacgaggg ctataccaag caaggcgcga
       61 acgacaacgt ctccggatgc gcgctgtcgc tcgagatggg ccgcgcctac ctccggctcg
      121 tggccgacgg cgctctgccg aagccgaaac gggcgattca cttcctctgg gtgcccgaga
      181 tcagcggcac catggcgtgg ctcagggcgc acgacgatgt cgcgaagcgg atcgtcgcca
      241 acctcaactt cgacatggaa gggctgctgc tgaggctcag cggcagcgcc tgggtcatgc
      301 accggacccc ggactcggtg ccgtcctttc tgaacgacct gtgcgccagc gtgctgcggt
      361 tcgtcggaga gaccaaccgc gagcgcgtgc gctaccgctc ggtggcctac ggattcacgc
      421 ttccggtcgt gtcgcagaac ggcagccggg acccgttctt cgtcatcgaa gacccgtact
      481 acggcgcgag cgaccacgtc gtgttcatca accagggcat tccggcggtg atgttcatca
      541 cctggccgga caacttctac cactcgtcgc aggacacgcc cgagcgcctc gaccccacgc
      601 agatgaagcg cgcggcggtc gtcggcatcg gctgcatgag cctggtggcg tcggccggcg
      661 acgagacggg cctgaaagtg gcggccgagt cgctggcgag gggcgccgag cggatgggcc
      721 aggcgcagcg caagggcgcg tcctacatcg cggacctctc gttcggcctc tccctcccgg
      781 aagcctacaa ggaggcgcgg acggccatcc gccatcaggc caacgtcgag aaggcggtgg
      841 tgaagtccgc ctcggtcctg ttcgccgatc cggcggccgc cgcgaagaag ctggcggcgt
      901 tcgacctgct catcgaccag cgcgcggcca gcctgcagaa cgagatcgcg gcgctctgcc
      961 gcctgcgggc ggagcagctg aagctgccgt gggccgaacc cgcgccgacc gacctggaga
     1021 agcgggccgc cgcgacgctg gtcgaagccc tgccgcagcc ggccggcgcg tcgcccttcg
     1081 gaggaggcgg ccggcgcgct cagacgggcc cgatgactcc ggccatgcag gcgatggcgg
     1141 acgcccggcg gaagatcccg gcccacatcc tcggtgaact cggcacggtc atgcggcagg
     1201 gccggacggt cctggaaatc cgggacttca tctcgggcga gttcgagccc gtgcccctgc
     1261 aggacgtctt cgactatctg aaggccgccg aggccctcgg gcaggtcagg ctggtcgaga
     1321 agccggctgc gccagcgacg aagaaggcgc ctgcgacagt gaaggccccg gcgcaggcag
     1381 gctgactccg atggcgcgtc aacacgaaca cgtgacgtac ttcacctacc tggacactcc
     1441 cgtcggcgtc ctcatgctgg cgggatgtcc ggaccacggc ctccggcatg tcgcattcca
     1501 gtgcggacaa ggcgcgctgg cgcccgagcc ggactggaag ccgtcgaaga agccgttccg
     1561 cgaggctgaa cgccagctca tcgagtactt ccatgggcgg cggactcggt tcgacctccg
     1621 gctcagcccg acgggcacgg cgttccagac ggcggtgtgg aaggccctgc agcggatccc
     1681 gtacggggaa acccggtcgt acggcgaaat cgcgaaggcc gtcggccgcc cgcgcgcggc
     1741 acgcgccgtc ggcctcgcca acgccaggaa ccccctgccg atcgtggtgc cctgccaccg
     1801 cgtcatcggc agcacgggca ggctcgttgg cttcggcggc ggcctgccgg tgaagcaggc
     1861 gctcctggat ctcgagcgca gggtcagccg cgcgcgtcga acacgacccg ccccccgacg
     1921 atcgtcgtcg taacccgggc ctcgcggacc ctgtccgggg cgatcgcgag gatgtcgtcg
     1981 gacaggatcg cgaggtcggc gaaggcgccg ggagcgacga cacccttccg gccgtcggtg
     2041 aactcggcgt acgccgaccc gctggtgtag cctcgcaacg cgtcttcgac ggtgatcttc
     2101 tggcgcggca cccacccttc gggatgggcc ccgtccagcg tcgcccgcgt ggccgccgcg
     2161 tagatgccga ggagcgggtt cagcggagcc accggccagt cggacccgag ggccaggcgc
     2221 acccccttct cgagaagcgt ccggaacggg tacgcggatc ggatgcggcg ggggccgatg
     2281 cgccgctccg cccatcgccc gtcgtcgatg gcgtggtagg gctggaccgc cgcgatgacc
     2341 ccgagcgacg cgaagcggtc gaagtcggcc tcggccacgt gctgcgcgtg ctcgatgcgg
     2401 agccgccggt cgcgcggacc gtttgccgcc acgacgtcag tgaacacgtc cagcacgatg
     2461 gagaccgcgc gatcgccgat cgcgtgcacg cacagctgga gcccggccgc gtccgcggcg
     2521 atgagccgcc gacgggcgtc ctccagcggc gtcagatcgt ctgcgagcag gccgatcgta
     2581 tccggcgcgt cggcgtacgg ctcgaagaac agcgccgtcg acgatccgag cgagccgtcg
     2641 gcgaacgcct tgacggcgcc caggctgagc caaggcgtgc cgaatgcgct acgggctccc
     2701 caattcgcct gctcctccca gcgcgcggcc ggcggggccg cagacacccg gagcgtcaag
     2761 cgccccgcgt cagcgagctc ggccaaggcc gccaggtcgt ccggctcggg ccccatgtcc
     2821 cgcacgctcg tgacgcccag cgacgccgcg tggtccagtg cgcgcctgag cgtcgccgtg
     2881 cgctgagcgc gagtgggggg aggaaccgcc cggacgacca gcgccatggc ggcgtccttc
     2941 agcactcccg tcgcccggcc gccccggtcg cgcacgatca cgccgccggg cgggtcgggc
     3001 gtccccgcgg cgatgcccgc gagttcgagc gcgagggaat tggccgcggc catgtggagg
     3061 tcgtaccgcg ccacgaagac gggcgtcgcc ggagccgcac gatcgagaag gtcccggtcc
     3121 ggcggcaggg cacgatccca gcgctgctcg tcccatcccc cgccaagcac ccacgatccc
     3181 gccggcgtga cggcggcgcg ctcgcctacg agacgggcga gctcagcggg cgacgcggcc
     3241 tcgcgcaggt ccacgctgtc gagctgccgc ccgccggcca tgaggtggac gtgcgaatcg
     3301 ttgaagcccg ggacgatcct ggcgcctcgc gcgtcgatga ccgtcgtggc gcggcctcgc
     3361 caccccgcca gcgccgatgt gggcccgacg gccgcgatcc ggtcgccggc gacggcaacc
     3421 gccccggcct cggagcggcc ggcgagcccc gtccacaccc tgccgttcaa cacgagcagg
     3481 tcggccggtc cggttcgcgc accggctccg cggcccgcgg ccccgggcgc tgccggcccc
     3541 ggctgcggcg ctgagtccat gacaccccca acaaaggtcg acggtgggat tatactgatg
     3601 ggtgcgcgtc ggctgcgccg gcggcgggcg cgcgcgaacg gccgtgagcc cccgcatggc
     3661 caaggcgaga tgggacggga ggttccggat ggaggatgcg ggtcagaagg cgtacaacgt
     3721 ccagcagttc caggatctcg tcaaggacat cccgtttgcg atgttcacga ccatgaacgg
     3781 cgagcgcggc ctgcggagcc gcccgatggt ggcgtctgct ggggcgttcg atggggacct
     3841 ctggttcttc actcgcacct cgtcggccgt cgcccaggaa ctcgccaaga acgcggtcgt
     3901 gaacatcacg tatgtctcgg cgccggagga tcggttcatt tcggtctcgg gcaacgcgtg
     3961 cgtggtgcgg gatgtcgagc acgccctggc catgtggcac ccggcctaca accagtggtt
     4021 ccccggcggc gccgccgacg ccgacctgtc gctcatcaag gtcgaggtca gacgcgtcga
     4081 gtactgggac cggaaggccg ggcgcatgaa ggacctcgag gcacgatagc gccccttgac
     4141 ttcgggcggc gaaacgccga tattacgggg ggctgttgag tcccaggcga acccgaggaa
     4201 gacggccgca cggtgtggag gcacatgccg gcgcggctgc aaggcaggtg acgccatgat
     4261 gaagcgagcg gtattcgtga tggtggccgt gcttctggcg ctggcgctgg gcgccgacgc
     4321 cggacaggga acgggcagga tctccggcac ggcgcgcgac gctcagaacc gggtgctggc
     4381 caacacaacg gttcagctgc gccatgtcga cacaggccag ctcgtggcga ccacgcggac
     4441 cgcgggagac gggcgattcg agttcaccgg cctgaacccg ggcaactacg tcgtggaaat
     4501 cgtcgacacc gacggcaaga tcatcggcct cagcccgtca acggccctgg cggccggcgg
     4561 gttcgtcagc gggctcgcgg tggcggcgag cgcagccggt gccgcggctg gggcgggcac
     4621 ggcctttttc gcctcgaccg ggggcattct ggtgctggcc ggtgccggca tcgccctgac
     4681 ggcaggcgtc atcgcgctca cccaggacgc cagcgcgtcc aagtagcccc ggcggctgcc
     4741 tcctggatac ggggcgggct ctctgcccgc ctcgggcgtt cgtgtggagc cgctgcggct
     4801 tcgcctcgag ttcgccgcgc cccgccccga tttctctcag ccgatcattc gtagcgcaag
     4861 gcctcgacag gatcgaggtt cgcagcccgc cgcgcgggaa gatagcccgc gacgagcccg
     4921 gtgcccaccg ccgagcccag cccgccagcg accgcccaca gcggaggcac cgcgggaaac
     4981 gccggggccg ccaggctcag ggcgaacccg agcgcccagg aaaccgcgac gccgacgccg
     5041 cctccgacgg ccgacaacag cgcggcctcc acgaggaact gggccagcac gtcgcggcgt
     5101 ttcgcgccga tggcgaggcg agtgccgatt tcccgcgtgc gctccgtcac ggcgatgatc
     5161 atgacgttgg cgatgccgat gccgccgatc acgaggctca ccagggccag ggcgaaggcg
     5221 gcgagcccga tctgggcgct gatcgcgtcg aggttcgaga tgatctgctc cgcggtggag
     5281 agggtgaagt cgttgtccgc tcctgggggc agcccccgca tgcgccggag caggaactcc
     5341 agctcggccc ggcccgctgc cagctggccc ggcgcggtgc gcgcgtagag cacggtg
//
LOCUS       DAOSIF010000173         5375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13644_length_5375_cov_5.044549, whole genome shotgun sequence.
ACCESSION   DAOSIF010000173 DAOSIF010000000
VERSION     DAOSIF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13644_length_5375_cov_5.044549"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="PLT35_07090"
     CDS             <1..1705
                     /locus_tag="PLT35_07090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG29030.1"
                     /translation="VGAFRFTPDGRRLVFTAEVWPECGADMACNRKRDDEAEKRKIRA
                     VVADRLLYRHWDAWEDGKRTHIMIMDLADAKAPLRDLTPGDFDSPAFVVGGAADFDVS
                     PDGKELVFTSNRDAQPADSTNSDLWIVPIDGDAALLAAPRNITAANRASDSAPRYSPD
                     GRSIVFTHQRVPGYESDRIVLASYDRASGAIRVLTERLDAWVRAFRFSPDGARIYFTA
                     DVRGRTPIHALDLATGAISVVSGVGHVDAFDVSKDGTWAAVARRRMHLPAEIYRVALS
                     GPSSGTERALTSQNQALLDEVDFRPMEEVFVDSPSGKRIQVLLVKPHGFDPAKQYPAI
                     VNVHGGPQMQWADAFRGDAQVYPGAGYVVAFPNPHGSTGWGQDFTAAISGDWDGKVMQ
                     DILAVADWLVSQPWVDRARMGAMGWSWGGYAMMWLQGHTDRFKALAAMMGVYDLRTMY
                     SSTEELWFPEWDLKGTPWQNPEGYRRQSPSEYVTRFKTPTLVLTGERDYRVPYTQSLA
                     FFTDLQKMGVPSRLVVFRNAGHWPGWYDMVLYYAAHLDWFHTYLGGGASPLDPKALAA
                     Q"
     gene            1725..3173
                     /locus_tag="PLT35_07095"
     CDS             1725..3173
                     /locus_tag="PLT35_07095"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG29031.1"
                     /translation="MRRALIVPILMAVWAPSAAARAGHPAPGQGAARPLQAAQSLGPH
                     EQAARDIFKELVEIDTTDSSGSTTRAAEAMASRLRAAGFPESDVLVVGPNARKGNLVA
                     RLRGRGARRPILLLAHLDVVEARREDWSPDLDPFALTERDGYFYGRGTSDDKNQASIW
                     IANLIRYKQEGFVPDRDIIVALTADEEGGDSNGASWLLANHRALVDAEYALNEGGGGD
                     LKDGRRIANNVQAAEKVYFSVALEARNPGGHSALPRRDNAIYQLSAALTRLAALEFPI
                     ELNEIAKAYFAGMAAQQTGQLAEDMKAVAGPSPDPAAAARLAAASPSYNAVMRSTCVA
                     TQLSGGHAENALPQSARAVVNCRLLPGADPRAVERALRDAIADDGISITPVSEPVPSP
                     ASPLRPDLMDAIRRHTDEMWPGVPVVPFMASSATDGMHFRNAGIPTYGVDGTFFDVDD
                     VRAHGKDERLGVREYFEGLEFLYRLVKTLSGP"
     gene            3259..4164
                     /locus_tag="PLT35_07100"
     CDS             3259..4164
                     /locus_tag="PLT35_07100"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOG29032.1"
                     /translation="MSIRPVKRLVTSTPTLEGAGVRLRRAFGFGETGDVDPFLLLDDF
                     RSDRPADYAAGFPWHPHRGIETITYVLAGTVDHSDSLGNAGTIGPGDVQWMTAGSGII
                     HQEMPHGNERGQMHGFQLWANLPSSLKMTAPKYRDVTAADIPEVIDDDGTAVKIISGS
                     FWGRQGPVRDVAAEPQYLDVFVPPGRRRSLPVPTTRHAFAYVFQGGGRFANASEPQAV
                     TTDVVGGEDAGSRYAENRSLVVFDSGDEVTVRAGEQGIRFLLVSGAPLGEPIAWRGPI
                     VMNTQEELRQAFEEFRSGTFLKHER"
     gene            complement(4185..4622)
                     /locus_tag="PLT35_07105"
     CDS             complement(4185..4622)
                     /locus_tag="PLT35_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOG29033.1"
                     /translation="MAADDVYRTIREKVSGYTGAYADYVMLVPDLFLLVTRLMLDQRV
                     EAKHKVYLGAAVAYVISPIDLLSERRFGPIGYLDDLVVIVAALNILLNESDQNVVLEH
                     WAGKEDLLGTVRNIVAQADGLIGKGRLEKILQTIGIRRPAPGV"
     gene            complement(4949..>5375)
                     /locus_tag="PLT35_07110"
     CDS             complement(4949..>5375)
                     /locus_tag="PLT35_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29034.1"
                     /translation="RDTETEAPKGLQAAFAKGNRLQDILLAEMKPGLTGNAILAAALK
                     KAKTEGIVGSIYSHPIGYHGHAAGTIIGLWDRQGGVPGLGDFPLHEDTAHAIELSVRA
                     VAPEFGNIDVRILLEQDILFRRDGAAWMDERQTSLMLIR"
BASE COUNT          785 a         1908 c         1895 g          787 t
ORIGIN      
        1 cgtgggcgcg ttccgcttca cgcccgacgg ccggcggctg gtcttcaccg ccgaggtctg
       61 gcccgaatgc ggcgccgaca tggcctgcaa ccggaagcgc gacgacgagg cggagaagcg
      121 gaagatccgg gcggttgtcg ccgaccgcct gctctaccgc cactgggacg catgggagga
      181 cggcaagcgg acccacatca tgatcatgga cctggctgac gcgaaggccc cgcttcgcga
      241 tctgacgccc ggcgacttcg actcgccggc cttcgtggtc ggcggcgccg cggacttcga
      301 cgtgtcgccc gacggcaaag agctcgtctt cacctccaat cgcgacgcgc agccggcgga
      361 ctccaccaac tcggacctct ggatcgtgcc gatcgacggc gatgccgcct tgctggcggc
      421 cccgaggaac atcaccgccg cgaaccgcgc ctcggacagc gcgccgcgct actcgcccga
      481 cgggcgctcc atcgtcttca cgcaccagcg cgtgcccggc tacgagtccg accggatcgt
      541 gctggcgagc tacgaccgcg cgagcggcgc gatccgcgtg ctgaccgaac ggctcgacgc
      601 gtgggtgagg gcgttccgct tctcgcccga tggcgcgcgg atctacttca ccgcggacgt
      661 caggggccgg acccccattc acgccctcga cctcgccacc ggcgccatct ccgtcgtctc
      721 gggcgtcggc cacgtcgatg cgttcgacgt gtcgaaggac ggcacgtggg cggccgtggc
      781 ccggcgccgg atgcacctgc cggcggagat ctaccgcgtg gcgctgagcg gcccttcctc
      841 cggcaccgag cgcgcgctca ccagccagaa ccaggcgctg ctcgacgagg ttgacttccg
      901 gccgatggag gaggtgttcg tcgattcccc gagcggcaag cgcatccagg tgctgctcgt
      961 gaagccgcac ggcttcgacc cggcgaagca gtacccggcc atcgtcaacg tgcacggcgg
     1021 cccccagatg cagtgggccg atgcgtttcg cggcgacgcc caggtctacc ccggggccgg
     1081 ctatgtcgtc gccttcccca atccgcacgg ctcgaccggg tgggggcagg acttcaccgc
     1141 cgcgatctcg ggcgactggg acggcaaggt gatgcaggac atcctcgcgg tggccgactg
     1201 gctggtctcg cagccgtggg tcgatcgggc gcggatgggc gcgatggggt ggtcctgggg
     1261 cggctacgcg atgatgtggc tgcaaggcca taccgaccgg ttcaaggccc tcgctgcgat
     1321 gatgggcgtg tacgacctgc ggacgatgta ctcgtcgacc gaggagctgt ggttcccgga
     1381 gtgggacctc aagggcacgc cgtggcagaa cccggagggc taccggaggc agagcccgtc
     1441 ggagtacgtg acgcgcttca agacgccgac gctcgtgctc accggcgagc gggactaccg
     1501 cgtgccctac acgcagtcgc tcgcgttctt cacggacctg cagaaaatgg gcgtgccctc
     1561 gcggctcgtc gtgttccgga atgccgggca ctggccgggc tggtacgaca tggtgctgta
     1621 ttacgccgcg catctcgact ggttccacac gtacctgggc ggcggcgcgt caccgctgga
     1681 tccgaaggcg ctggccgcgc aatgagccag cggagcattc agccatgaga cgtgcactca
     1741 tcgttccgat cctgatggcc gtctgggcgc cttctgcggc ggctcgcgcc gggcatccgg
     1801 cgcccggcca gggcgccgcg cggccgctgc aggcggcgca gagcctcggc ccgcacgaac
     1861 aagcggcccg cgacatcttc aaggagctcg tcgagatcga cacgaccgac tcgagcggca
     1921 gcacgacccg ggccgccgag gccatggcgt cgcggctccg cgcggccggg ttccccgaga
     1981 gcgacgtgct ggtcgtcggt cccaacgccc gcaagggcaa tctggtcgcg cgtctgcggg
     2041 ggcgcggcgc gcggcgaccg atcctgctgc tggcccacct cgacgtcgtc gaggcccgcc
     2101 gggaggactg gtcgcccgat ctcgatccgt tcgcgctcac cgagcgcgac gggtacttct
     2161 acggccgggg cacgagcgac gacaagaacc aggcctcgat ctggatcgcc aacctgatcc
     2221 gctacaagca ggagggcttc gtcccggatc gcgacatcat cgtcgcgctc acggcggacg
     2281 aagagggcgg cgactcgaac ggggcgagct ggctgctcgc gaaccaccgg gcgctcgtcg
     2341 acgcggagta cgcgctgaac gaaggcggag ggggcgacct caaggacggc aggcggatcg
     2401 ccaacaacgt ccaggcggcc gagaaggtgt acttctcggt cgcgctcgag gcgcggaacc
     2461 cgggaggaca cagcgcgctg ccgcgccggg acaacgcgat ctaccagctg tcggccgcgc
     2521 tgacgcggct ggccgccctc gagttcccga tcgagctcaa tgagatcgcg aaggcgtact
     2581 tcgcggggat ggccgcgcag cagaccggcc agctggccga ggacatgaag gccgtcgccg
     2641 gcccgtcacc cgatccggcg gccgcggccc ggctcgccgc ggcgtcgccg tcctacaacg
     2701 ccgtgatgcg atccacctgc gtcgccaccc agctgagcgg cgggcatgcg gagaatgcgc
     2761 tgccgcagtc ggcgcgggcc gtcgtgaact gccggctcct gccgggcgcc gatcccaggg
     2821 ccgtcgagcg cgcgctgcgt gacgcaatcg cggacgacgg gatctccatc acgccggtct
     2881 cggagcccgt cccgagcccg gcgtccccgc tccggccgga tctgatggac gcgatccggc
     2941 gtcatacgga tgagatgtgg cccggggtgc cggtggtgcc cttcatggcc agcagcgcga
     3001 ccgacggcat gcacttcagg aacgccggca ttcccaccta cggcgtggac ggcacgttct
     3061 tcgacgtgga cgacgtgcgc gcgcacggca aggacgagcg gctcggcgtg cgggagtact
     3121 tcgagggcct cgagttcctc taccgtctgg tcaagacgct gtccgggccc tgagtcggcc
     3181 ggacaggcgg ggccggatcc gcggacgagc gggcccggtc acgcgacggc gcggccgcgg
     3241 ggcgcgaggg ggagcagcat gtcaatccgt ccggtcaaac ggctcgtcac ctccacgccc
     3301 acgctcgaag gggccggcgt ccgccttcgg cgcgcgttcg gcttcggcga gaccggcgac
     3361 gtcgatccct tcctcctgct cgacgacttc cgcagcgacc gccctgccga ctacgcggcg
     3421 ggctttccgt ggcacccgca ccgcggcatc gagacgatca cgtacgtgct ggccggcacc
     3481 gtggaccaca gcgacagcct ggggaacgcc ggcacgatcg gccccggcga cgtccagtgg
     3541 atgacggccg gcagcggcat catccaccag gagatgccgc acggcaacga gcgcggccag
     3601 atgcacggtt tccagctctg ggccaacctg ccctcgtcgc tcaagatgac cgcgccgaag
     3661 taccgcgacg tcacggccgc ggacattccc gaggtgatcg acgacgacgg cacggcggtg
     3721 aagatcatct cgggctcgtt ctggggccgc caggggcccg tccgggacgt tgccgccgag
     3781 ccgcagtacc tcgacgtgtt cgtgccgccc ggccggcgcc gcagcctccc ggtcccgacg
     3841 acgcgccacg cgttcgccta cgtgttccag ggcggcggcc ggttcgcaaa cgcgtcggag
     3901 ccgcaggcgg tgacgacgga cgtcgtcggc ggcgaggacg ccggcagccg gtacgccgag
     3961 aaccgttcgc tcgtggtgtt cgactcgggc gacgaggtga cggtgcgggc gggggagcag
     4021 ggcatccggt tcctgctcgt gtcgggcgcg ccgctcggcg agccgatcgc ctggcgcggc
     4081 cccatcgtca tgaacacgca ggaggaactg cgccaggcgt tcgaggagtt cagaagcggg
     4141 acgttcctga agcacgaacg ctgaaccgac ggagcccggg gccttcagac cccgggggcc
     4201 ggccttcgga tcccgatcgt ctggaggatc ttctcgagcc ggcccttgcc gatcagcccg
     4261 tccgcctgcg cgacgatgtt ccgcaccgtt cccagcaggt cttccttgcc ggcccagtgc
     4321 tccaggacga cgttctggtc cgactcgttg agcaggatgt tcagcgcggc cacgatgacg
     4381 accaggtcgt cgaggtagcc gatcgggccg aagcggcgct ccgacagcag gtcgatgggc
     4441 gagatcacgt acgccacggc ggcgcccagg tagaccttgt gcttggcctc gacgcgctgg
     4501 tcgagcatca ggcgcgtgac gagcaggaac aggtcgggca ccagcatgac gtagtcggcg
     4561 tacgcgccgg tgtagcccga cactttctcg cgaatcgtgc ggtagacgtc gtctgcggcc
     4621 atgtgtcctg acctcccggg tgccggcggg ccgtcggcgc ccctgtaggg gatcttacgg
     4681 aatccgcgcc agggagttgc ggtgtccctc cgcgccagtc cgacaggggc caggccgcat
     4741 cgcctcgcgc cgtcccgctc gcgcaggccg gcagcaggca cttccagggt gccgtcggcg
     4801 gcggcgctcg gtccctgtca cccgacgcgg acttcggccc ctgcggcgcg cttcgcagcc
     4861 tgacgcccct cggcctggtt cgagggcctg ctcgaaacga ccctgcccgg ccttccgggg
     4921 accccgagga gccctggtcc gcccgcggct atcgaatcag catcagcgac gtctggcgtt
     4981 cgtccatcca cgccgccccg tccctgcgga acaggatgtc ctgctcgagc aggatccgga
     5041 cgtcgatgtt gccgaactcc ggcgcgaccg cgcgcacgct cagctcgatg gcgtgcgccg
     5101 tgtcctcgtg gaggggaaag tcgccgagcc ccggcacgcc gccctgcctg tcccacaggc
     5161 cgatgatcgt gccggccgcg tggccgtggt acccgatcgg gtgcgagtag atgctcccga
     5221 cgatgccctc cgtcttcgcc tttttcaggg cggcggccag gatcgcgttg cccgtcaacc
     5281 ccggcttcat ctcggccagc aggatgtcct gcaggcggtt gcccttggcg aacgcggcct
     5341 ggaggccctt cggcgcttcc gtctcggtgt ccctg
//
LOCUS       DAOSIF010000174         5373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13650_length_5373_cov_4.482136, whole genome shotgun sequence.
ACCESSION   DAOSIF010000174 DAOSIF010000000
VERSION     DAOSIF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13650_length_5373_cov_4.482136"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLT35_07115"
     CDS             <1..990
                     /locus_tag="PLT35_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG29035.1"
                     /translation="SKVVGSLRISGSELKNAVGQLAAGFQMFHPEAKLTISHTTSSEG
                     GIAGLYMFTSDVAPMGDDAKITDMMPFYNTFGYMPTEISVATGGYEKRGSLWAWAIVV
                     NKDNPLDHISVEELKRVFGAERSGGWEMAENDYRFTSKYARTKDTNIRTWDQLGLTGE
                     YAGREIETFGYAAPGFATAIERSWFHWSKKWNPNFMEYVEAKQTTPDEAGAKVASTVP
                     LAQLATNRFAIGIAGLMHVKNYPHIKVLKISREPGGQAFALTPENVAKRAYPLIRDAY
                     FYVNKPPGQKLDPKVREFMRFVLSREGQEIIARVGYYYPLDAAYLKEQLKKLD"
     gene            complement(987..1172)
                     /locus_tag="PLT35_07120"
     CDS             complement(987..1172)
                     /locus_tag="PLT35_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29036.1"
                     /translation="MPFKTNAAGPLSVGASPVGGGEHADIHRTTAAKAAVAAAAFALR
                     LLSLALVSDIPASILLV"
     gene            1162..2292
                     /locus_tag="PLT35_07125"
     CDS             1162..2292
                     /locus_tag="PLT35_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29037.1"
                     /translation="MKGIAEPLKTAEGKDAPGSAAERLARQKKLGRSYSTRFDLAALP
                     EYVPARQVSGTIRIWGNNYIDASGLAKAWTDEFRTFHPGAAFELVLPTAAAAIPALYF
                     GLADLAMTHQPTFYDSLTHLRILGYAPTGFMAVTGSFDQSGWMNSIAIAVHKDNPIDK
                     ITLQQLDGIFGSARMGGWDGTTWRADWARGPERNIRTWGQLGLTGAWARQRITPYGYS
                     VTYSTAQAFSRMVLQDGDRWNEDLQDFGNIRNPDGTQTLQATQIIDRLKADPHGIAYI
                     RYQKGFADVGLKYLPVARDGEGPYVPFTIENIQNRTYPLAEEMNFWVSVKPGTKMDPK
                     VKEFLRYVFSRQAQNLVQEVDGKYLPLCADIVRRELARLDQY"
     gene            2525..3502
                     /locus_tag="PLT35_07130"
     CDS             2525..3502
                     /locus_tag="PLT35_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG29038.1"
                     /translation="MRFVDALPPYEPHGRVDGPVRLWGHGSFRHDFMRKLVTSWIAQF
                     SRVQPGVTFDYRMYGTASAVGAVCTNAGDIAILGEEISPAAARMFLRAKGYPHTDISI
                     ATGSLDVNFFDYAHMVFVHRDNPLSRLSLADLDGIFGAEHRRAGRNIRVWGDLGVTGA
                     RAGRTIRPYGWKTDVDFALFFRERVLGDSHRWNPEIREFVHITRPDGTQYDHGQQILD
                     ALAADPDGIAISNVRYATPAVKALALAWTREGPYVEATPASLISQEYPLVRIIPAIID
                     RAPGQPIAPPVREFLRFVLSREGQRSLVEESGYLPLGPKVIQRQLEKLR"
     gene            3499..4500
                     /locus_tag="PLT35_07135"
     CDS             3499..4500
                     /locus_tag="PLT35_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG29039.1"
                     /translation="MKGLALALFLLAVAHAAAAQIPPPSHEPPPGVVRVWGHAQARNL
                     VRRWAASFETAHPGVRIDARLTGSDIGFAGLYTGQADIALVGREATDSETKAFEWVFR
                     YKPASVQVMTGSLDRAGRSPAIVVFVHRHNPLERLSLAQLAAIFGHGEGPLGAIRTWG
                     QLGLTGTWADAPIRLYAPMAESGTGRAFRARVLGGSTKMNWDHLREFADEVSQPDGID
                     TSGARIAAALASDRYGLALSNLGSARPELKPIALASAGDGEYCHATRVNVIDRRYPLA
                     RSIYAYVNQRPGVALAPSVRLFLKHILSDHGQAVVSDYGYLRLNARILAEQRRLVDR"
     gene            complement(4561..>5373)
                     /locus_tag="PLT35_07140"
     CDS             complement(4561..>5373)
                     /locus_tag="PLT35_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG29040.1"
                     /translation="DGAEPIITETLSFYEVYGYHATQITVATGAYDAEGRSNGLIVFV
                     HKDNPISRLTMRQLDGIFGAERNGALRGFKWTMEDGRGPEGNIRTWGQLGLSGEWANK
                     AIQTYGHAPSGTARFFQWKVLRNSGKWNSNYREYVETGSKMIGDDDRVAQRGGLKHML
                     ANELANDRYGIAWTVLPQAQGIAGIKPVALASADGGEYYEPSAKSFQDRTYPLARSIY
                     IYLNRPPGQPLEAKIAEFLRYVLSRDGQQLVAKTGYLPLTASLAHEQLGQLQ"
BASE COUNT          922 a         1807 c         1799 g          845 t
ORIGIN      
        1 tccaaggtgg tgggaagctt gcgcattagc gggagcgagc tgaagaacgc cgtggggcag
       61 ctcgccgcgg gcttccagat gttccacccc gaggcgaagc tgaccatcag ccacaccacg
      121 agcagcgagg ggggcatcgc cggcctgtac atgttcacgt ccgacgtcgc cccgatgggc
      181 gacgacgcca agatcaccga catgatgccg ttctacaaca cgttcggcta catgcctacg
      241 gagatctcgg tcgcgacggg cggttacgag aagcggggtt ccctgtgggc ctgggcgatc
      301 gtcgtcaaca aggataaccc gctggatcac atctcggtag aagaactgaa gcgggtcttc
      361 ggcgccgagc gctccggcgg gtgggagatg gccgagaacg actaccgctt cacgtcgaag
      421 tacgcgagga cgaaggacac caacatccgc acctgggatc agctcggcct gactggtgag
      481 tacgcgggcc gcgagatcga gacgttcgga tacgccgcgc cgggcttcgc caccgccatc
      541 gaacgcagct ggttccattg gtcgaagaag tggaacccga acttcatgga gtacgtcgaa
      601 gccaagcaaa cgactcccga cgaggctggc gcgaaggtgg ccagcaccgt gcccctggcg
      661 cagctggcga cgaacaggtt cgccatagga atcgcgggcc tgatgcacgt caagaactac
      721 ccgcacatca aggtcctgaa aatcagccgc gagccggggg ggcaggcgtt cgcgctcacg
      781 cccgagaacg tcgcgaagcg cgcgtacccg ctgatccgcg acgcgtactt ctacgtgaac
      841 aagcctcctg gccagaagct cgaccccaag gtgcgcgagt tcatgcgctt cgtgctgagc
      901 cgtgaagggc aggagatcat cgcccgcgtc ggctactact acccgctcga tgcggcctac
      961 ctcaaggagc agctgaagaa gctggactag acaagcagga tggaggcagg aatgtcggaa
     1021 accagcgcca gggacagcaa gcgcagagcg aacgcagcgg cggcgaccgc tgccttcgcc
     1081 gccgtcgtcc tgtggatgtc ggcgtgctcg ccgccgccaa ccggcgaggc accgaccgag
     1141 agcggtccgg ctgcattcgt cttgaagggc atcgccgagc cgctcaagac cgcagaaggc
     1201 aaggacgcgc cggggtccgc cgcggagcgc ctggctcgcc agaagaagct gggccgcagc
     1261 tattcgacca ggttcgatct tgcagccctg ccggagtacg tgccggcgcg gcaggtcagc
     1321 ggcacgatcc ggatctgggg caacaactac atcgacgcgt cgggcctcgc caaggcctgg
     1381 accgacgagt tccggacgtt tcacccgggc gcggcgttcg aactagtgct gcccacggcc
     1441 gcggccgcca ttccggccct gtacttcggc ctggcggacc tggcgatgac ccatcagccg
     1501 acgttctacg actcgctgac gcacctgcgg attctcggct acgcgcccac gggattcatg
     1561 gccgtcaccg ggtcgttcga ccagtccggc tggatgaact ccattgcgat cgcggtgcac
     1621 aaggacaacc cgatcgacaa gatcaccttg cagcagctcg acgggatctt cggcagcgca
     1681 cgcatgggag gctgggacgg gacgacctgg cgggccgact gggcgcgggg ccccgagcga
     1741 aacatccgga cgtgggggca gcttgggctg accggcgcgt gggcgcggca gcgcatcacg
     1801 ccgtacggct acagcgtcac ctacagcacg gcccaggcgt tctcgcggat ggtcctgcag
     1861 gacggcgacc gctggaacga agacctgcag gacttcggca acattcggaa cccggacggc
     1921 acgcagacgc tgcaggccac gcaaatcatc gacaggctga aggccgatcc gcacggaatc
     1981 gcatatatca ggtaccaaaa ggggttcgcc gacgtcggcc tgaagtacct gccggtggcc
     2041 agggacggag agggcccgta cgtgccgttc acgatagaga acatccagaa caggacgtac
     2101 ccgctggccg aggagatgaa cttctgggtg agcgtgaagc cgggtacgaa aatggatccg
     2161 aaggtgaagg agttccttcg ttacgtcttc agccgacagg cgcagaacct cgtgcaggag
     2221 gtggacggca agtatctccc tctctgcgcg gacatcgtgc ggcgggagct ggcccgtctg
     2281 gatcagtact gaccggtcgc ttggcgcatc gaacgcctcg gacgccatgg ctgcccatcc
     2341 tggccgtggc acttcgggcc gccgggctcc cgtccaggag cttcgtcggg cgacagcgat
     2401 gacgcgcaag ccagctctcc cgcctgctct ggcccgaatt gcctgcctcg ttctcctgct
     2461 gctcgcgcca gccggcgtga gcagcagggc ggtacggacc ggccagccgc cgctacggga
     2521 tgccgtgcgg ttcgtcgatg ccctcccgcc gtacgagcct cacggccggg tcgacgggcc
     2581 cgtcaggctc tgggggcacg ggagcttcag gcacgacttc atgcgcaagc tcgtcacgag
     2641 ctggattgcg cagttcagcc gagtccagcc cggggtgacc ttcgactacc ggatgtacgg
     2701 aacggcatcc gcggtcggtg ccgtctgcac gaacgccggc gacatcgcca tcctcggcga
     2761 ggagatcagc ccggcggcag cccggatgtt cctgcgcgcg aagggctacc cgcacaccga
     2821 catctccatc gcgacgggca gcctcgatgt caacttcttc gactacgctc acatggtctt
     2881 cgtccaccgg gacaacccgc tcagccggct gtctctggcg gacctcgacg ggatcttcgg
     2941 ggcagagcac cgccgtgcgg ggcggaacat cagggtgtgg ggggacctcg gcgtcacggg
     3001 ggcccgggcc ggaaggacca tccggcccta cggctggaag acggacgtgg acttcgcgct
     3061 gttctttcgc gagcgggtgc tcggggacag ccaccgctgg aaccccgaga tccgggaatt
     3121 cgtccacatc acgcgcccgg atggcactca gtacgatcac ggccagcaga tcctcgacgc
     3181 gctggccgcg gacccggacg gcatcgccat ctccaacgtg cggtatgcca cgcccgcagt
     3241 caaggccctc gcgctggcct ggacccgcga gggcccctat gtcgaggcga caccggcgag
     3301 cttgatctcg caggagtacc cgctggtgcg gatcatcccg gccatcatcg accgggcgcc
     3361 cggccagccg atcgcgcccc cggtgcgcga gttccttcgg ttcgtcctga gcagagaggg
     3421 ccagcggtca ctggtcgaag agagcgggta ccttcccctg gggccgaagg tgatccagcg
     3481 gcagttggag aagcttcgat gaagggcctc gcgctcgccc tcttcttgct tgcggtcgcc
     3541 cacgccgcgg cggcccagat ccctccgccc tcgcacgagc cgccgccggg cgtggtccgc
     3601 gtctggggac acgcacaggc ccggaacctg gtccggcggt gggcggcgag cttcgagacg
     3661 gcgcaccccg gcgtcaggat cgatgcgcgg ctgaccggga gcgacatcgg attcgccggc
     3721 ctctacaccg gccaggcgga catcgcgctc gtgggccgcg aagcgactga cagcgaaacc
     3781 aaggcgttcg aatgggtctt tcgctacaag cccgcctcgg tgcaagtgat gacgggcagc
     3841 ctggatcggg cgggccggtc gccggcgatc gtcgtgttcg ttcaccgcca caacccgctc
     3901 gaacgcctga gcctcgccca gctggccgcg attttcgggc acggcgaggg cccgctcggc
     3961 gcgatacgca cgtggggcca gctcgggttg acaggcacgt gggcggacgc gccgatcagg
     4021 ctgtacgcgc ccatggcgga gagcggcacg ggcagggctt tccgcgcacg cgtcctcggc
     4081 ggcagcacca agatgaactg ggaccacctg agggaattcg ccgacgaagt gtcccagccc
     4141 gacggcatcg acaccagcgg cgcgcggatc gccgcagccc ttgcctcgga tcggtacggc
     4201 ctcgctctct cgaacctggg ctctgcccgt cccgaactca agccaatcgc gctggcatcg
     4261 gccggagacg gcgagtactg tcacgccacg cgggtcaacg tgatcgatcg gcgttaccct
     4321 ctggcacgca gcatctatgc gtacgtcaac cagcggccgg gcgtcgccct ggcaccctcg
     4381 gtgaggctct tcctgaagca catcctgagc gaccacgggc aagcggtcgt gtccgactac
     4441 ggctatctgc ggctcaacgc ccggattctg gccgagcagc gccggctggt ggaccgctga
     4501 cagggccaca ggccgcgccg ccccagccgg cacccgccgc tggcccgtcg gcccgggagc
     4561 ctactggagc tgcccgagct gctcgtgtgc gaggctggcg gtcaacggga ggtatcctgt
     4621 cttggccacc agttgctggc cgtcccggct cagcacgtaa cggaggaact cggcgatctt
     4681 cgcttcgagc ggctggcccg gcgggcggtt caggtagatg tagatgctcc gggcaagcgg
     4741 gtaggtccga tcctggaagc tcttcgcgct gggctcgtag tactcgcccc cgtcggcgga
     4801 ggccagggcc accggcttga ttccggcgat gccctgggcc tggggcagga ccgtccacgc
     4861 gatcccgtag cggtcgttgg ccagctcgtt ggccagcatg tgcttcaggc ccccgcgctg
     4921 cgccacgcgg tcgtcgtcgc cgatcatctt gctcccggtc tccacgtact cccggtagtt
     4981 ggagttccat ttgccgctgt tgcgcaggac cttccactgg aagaacctcg cggtgccgga
     5041 aggcgcgtgc ccgtatgtct ggatcgcctt gttggcccac tcgccgctca ggccgagctg
     5101 gccccacgtc cggatgttgc cttccggtcc ccgcccatct tccatcgtcc acttgaagcc
     5161 ccggagcgcg ccgttccgct cggccccgaa gatcccgtcc agttgcctca ttgtgagccg
     5221 agagattggg ttgtccttgt gaacgaagac gatgagaccg ttcgatcgcc cctcagcgtc
     5281 atacgctccc gtcgcgaccg tgatctgggt ggcgtggtat ccgtacacct cgtaaaacga
     5341 gagcgtctcg gtgataatcg gctccgcgcc gtc
//
LOCUS       DAOSIF010000175         5333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13785_length_5333_cov_4.819629, whole genome shotgun sequence.
ACCESSION   DAOSIF010000175 DAOSIF010000000
VERSION     DAOSIF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13785_length_5333_cov_4.819629"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..3522
                     /locus_tag="PLT35_07145"
     CDS             202..3522
                     /locus_tag="PLT35_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOG29041.1"
                     /translation="MLRSIRFALPVVLTLLVAPITVSTQPGARDTRLLAQPATSGTHV
                     AFVYADDLWTARLDGSDVRRLTTDLGAEGSPAFSPDGKLLAFSAQYDGNVDVYVLPVE
                     GGVPRRLTWHPGADLVQGFTPDGASVLFTSGRAAFTGAHQHLFTVPVAGGIETQLPIP
                     NANQAAYSPDGTRIAYVPLPPAFQQWKQYRGGRVSRLWLYSVADHAIEKVPQPPERSN
                     DVDPMWIGGTVYFRSDRDGEFNVYGYDTASKQVRRLTSHGDFPVMKASAGGGKIVYEQ
                     AGWLHLLDPATGASRRLAIGVVADLRETRPRFVKGPDYIRNADISPSGARAVFEYRGE
                     IVTVPAEKGDARNLTNSPGAHERAPAWSPDGTRIAYFSDASGEYELHVAPQDGKGEVK
                     RFRPGGAGFYSAIRWSPDSRRLAFQDNSQSLFWIDLATGAVKRVGGNRIVSPAQPVSA
                     DWSPDSKWLAYVVDTQPLVNTVFVYSTEQDKSFAVTDGLSEVTEPVFDKGGKYLYLFG
                     STDAGPVQDWFALSNQDMRRTRSLYVAVLRKGLPSPLAKESDEEKPKKADGAGGKPEA
                     AKPGDTTAAEPAADAKAGGDAKEPFSIDLDGLQTRIVALPIPAGDLSSLKVGDAGHVY
                     YLVRAGGESSLHHFNLATRKDETPLSSVDSFALSADAKKILYAKGRAWFITALAPKIA
                     PGEGRIAAGDIEVRIDPRAEWTQIFNEAWRINRDYFYAANMHGVDWRAMREKYAAFLP
                     DLATRDDLNRVIQWMCSELAVGHHRVGGGDRLDQPKNVPGGLLGADYEIANGRYRFKK
                     VFGGLNWTPDLRAPLTEPGVDVKAGEYLLAVNGRDVRPPANLYSFFENTSGRITELTV
                     GPNPDGSGSRTVQAVPVASETALRNRDWVEGNLAKVDRATGGRVAYVYVPNTSTAGHA
                     SFKRYFFPQSHKDAVIVDERFNGGGLVADYYIDILRRPFISNWHMRYGDDLKTPTASI
                     QGPKVMIIDEMAGSGGDLLPWMFRKFKLGPLVGQRTWGGLVGTLGFPILMDGGRVTAP
                     NLAIWTPEDGWVVENEGVPPDIEVEQTPAAVIAGRDPQLEKAIEVAMAELKKAPPTPQ
                     KRPPYPVRGRTQRGTFKK"
     gene            complement(3759..4472)
                     /locus_tag="PLT35_07150"
     CDS             complement(3759..4472)
                     /locus_tag="PLT35_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOG29042.1"
                     /translation="MHVWRADLDEPGWTNPVHAAFLDDSERARAARLIQPRRRRRFCA
                     AHAMLRTVLSRYVEGAGPASLAFVSGATGKPELDAGNASPPVRFNLSHSDGVALVAVA
                     RACAVGIDVEGARRDVRIEEIARRQFHPDVVRALLGVDPPRRAAAFLRCWCALEARLK
                     ASGTGFAAAGAGLMAQMGLPEPLLASLDAAEWAGVAGPWALSALDAGPGFAAVVAAEA
                     AAVAATEAWLETFAASPLP"
     gene            complement(4987..>5333)
                     /locus_tag="PLT35_07155"
     CDS             complement(4987..>5333)
                     /locus_tag="PLT35_07155"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOG29043.1"
                     /translation="VQVLDNVDATDNKIASHLAGSLYDMLAADPATVKPVGEWNTIVV
                     RVKDGKVTHTQNGTKVVEYTLWTPEWDRMVANSKFKTFPGFTEGIAKEGYIGLQDHGY
                     AVWFRNIKIRTL"
BASE COUNT          837 a         1904 c         1853 g          739 t
ORIGIN      
        1 aactggttcg acaacgccgc gcacgaagtg gccggtcact gcgcgacgca caggactgta
       61 agttccggac agtcgcgcgc gaaattcgtc ccgacccctt tgcctttgcg aacctgctgc
      121 gcccaatcgg cgtctaacgt ccagagccgc cggccgtgct ctgcgcgccc gccccttggc
      181 gattcctctt ggaggcctgc gatgttgcga tcgatccgat tcgccctccc ggtcgtcctc
      241 acgctgctcg tcgccccaat caccgtctcc acccagccgg gcgcgcgcga cacgcggctg
      301 ctggcccagc cggcgacaag cggcacgcac gtggccttcg tctacgcgga cgacctgtgg
      361 acggcccgcc tcgacggcag cgacgtgcgg cgcctgacaa cggacctcgg ggccgagggc
      421 agcccggcat tctcgcccga cgggaagctg ctggccttct ccgcccagta cgacggcaac
      481 gtggatgtct acgtgctgcc cgtcgagggc ggcgtcccga ggcgcctcac gtggcacccg
      541 ggcgccgacc tcgtgcaggg gttcacgccc gacggcgcct ccgtcctgtt cacgtccggc
      601 cgcgccgctt tcacgggcgc gcaccagcac ctcttcaccg tgccggtggc gggaggcatc
      661 gagacgcagc tgccgattcc gaacgccaac caggccgcgt actcgcccga cggcacacgg
      721 atcgcctacg tgccgctgcc gccggccttc cagcagtgga agcagtaccg cggcgggcga
      781 gtgtcgcggc tgtggctgta cagcgtggcc gaccacgcca tcgagaaagt gccccagccg
      841 ccggagcggt cgaacgacgt ggacccgatg tggatcggag gcacggtcta cttcaggtcg
      901 gaccgggacg gcgagttcaa cgtgtacggg tacgacacgg cgtcgaagca ggtccgccgt
      961 ctcaccagcc acggcgattt ccccgtgatg aaggcgtcgg cgggcggcgg caagatcgtg
     1021 tacgagcagg ccgggtggct ccatctcctc gacccggcca ccggcgcctc gagaaggctc
     1081 gcgatcggcg tcgtggcgga cctccgcgag acgcggccga ggttcgtgaa ggggccagac
     1141 tacatccgga acgccgacat ttctccctcg ggcgcccggg ccgtcttcga gtatcgcggc
     1201 gagatcgtga cggtcccggc cgagaaggga gacgcgcgca acctgacgaa cagccccggc
     1261 gctcacgagc gcgctccggc atggtcgccg gacggcactc gcatcgccta tttctctgac
     1321 gcgtccgggg aatacgagct gcacgtcgcg ccccaggacg gcaagggcga agtcaagcgc
     1381 ttcaggccgg gaggggcggg tttctactcg gccatccgct ggtcgccgga cagccggcga
     1441 ctcgccttcc aggacaattc gcagtcgctc ttctggatcg acctcgccac cggcgcggtg
     1501 aagcgcgtgg gcggcaaccg gatcgtcagc ccagcacagc ccgtcagcgc cgactggtcg
     1561 cctgattcca agtggctggc ctatgtcgtg gacacgcagc cgctcgtgaa caccgtcttc
     1621 gtctactcga cagagcagga caagtccttc gccgtcaccg acggcctgag cgaggtgacc
     1681 gagccggtgt tcgacaaggg cgggaagtac ctctacctgt tcggatcgac ggatgccggg
     1741 ccggtgcagg actggttcgc cctgtcgaac caggacatgc gccggacccg gagcctgtac
     1801 gtcgccgtgc tgcgcaaggg cctcccatcg cccctcgcga aggagagcga cgaggagaaa
     1861 ccgaagaagg ccgacggcgc cggcgggaag cccgaggcgg cgaagcccgg cgacacgacc
     1921 gcagcggagc cggcagccga cgcgaaggcc ggcggggacg cgaaggagcc gttctcgatc
     1981 gacctcgacg gcctccagac ccgcatcgtg gcgctgccga ttcccgccgg ggatctctcg
     2041 agcctcaagg tcggcgacgc ggggcacgtc tactacctgg tccgggccgg cggcgagtcg
     2101 tcgctccatc acttcaacct cgccacgcgc aaggacgaga cgccgctgtc gtctgtcgac
     2161 agcttcgccc tctcggccga cgcgaagaag atcctctatg cgaagggccg ggcgtggttc
     2221 atcaccgcgc ttgcgccgaa gatcgctccc ggcgaagggc ggatcgccgc gggggacatc
     2281 gaggtccgca tcgacccgcg cgccgagtgg acgcagatct tcaacgaggc ctggcgcatc
     2341 aaccgcgatt acttctacgc ggcgaacatg cacggcgtgg actggcgggc catgcgcgaa
     2401 aagtacgcgg ccttcctgcc ggacctggcg acccgcgacg acttgaaccg cgtcatccag
     2461 tggatgtgca gcgagctggc cgtcggtcac caccgcgtgg gcggcggcga ccgcctggat
     2521 cagccgaaga acgtgccggg cggcctgctc ggcgcggatt acgagatcgc caacggccgc
     2581 taccgcttca agaaggtgtt cggcggcctc aactggacgc cggacctgcg cgcgccgctc
     2641 accgagcccg gcgtggacgt gaaggccggc gagtacctgc tggccgtcaa cggccgcgac
     2701 gtgcggccgc cggcgaacct gtacagcttc ttcgagaaca cgtccggcag gatcacggaa
     2761 ctgaccgtgg gcccgaaccc ggacggatcc ggctcgcgca cggtgcaggc ggtgcccgtc
     2821 gcctcggaaa cggcgctgcg caaccgcgac tgggtcgagg gcaacctcgc gaaagtggac
     2881 cgcgcgacgg gcgggcgcgt ggcctacgtc tacgtgccga acacctcgac ggccggccac
     2941 gcatcgttca agcggtactt cttcccgcag tcccacaaag acgccgtcat cgtggatgaa
     3001 cggttcaacg gcggcggctt ggtcgccgac tactacatcg acatcctccg ccggccgttc
     3061 atcagcaact ggcacatgcg gtacggtgac gacctgaaga cgccgaccgc gtccatccag
     3121 gggccgaagg tgatgatcat cgatgaaatg gccggatccg gcggcgacct cctcccgtgg
     3181 atgttccgca agttcaagct cggcccgctg gtcggccagc gcacctgggg cggcctcgtc
     3241 ggcacgctcg ggttcccgat cctgatggac ggcgggcggg tcaccgcccc gaacctcgcg
     3301 atctggacgc cggaagacgg ctgggtcgtc gagaacgagg gcgtgccgcc ggacatcgag
     3361 gtggagcaga cgcctgccgc cgtgatcgcc gggcgcgacc ctcagctgga gaaggcgatc
     3421 gaggtcgcga tggcggagtt gaagaaggcg ccgccgacgc cgcagaagcg cccgccgtac
     3481 ccggtgcgcg gcaggacgca gcgcgggacg ttcaagaagt agcgggcctc cggcggtcga
     3541 ggggccgtgc cggcacccgg ccttcgggct ggaaggacgc agcacggcgc tgccggagcc
     3601 gggggcccgg aaacgggacc attcgagcga gccgtcccgc tcgatggccg tgccatcgcg
     3661 ccacggggcc ggcggcgtcc tcggcgttcg cccgcagacg atgaaaacca tgcgggggcc
     3721 ggcgatcaga aagctcgccg gctcgccgat cgaaggcgtc aggggagcgg cgacgccgcg
     3781 aacgtctcga gccaggcctc ggtggccgca accgcagccg cctcggccgc gactaccgcc
     3841 gcgaagccgg gaccggcgtc gagcgccgac agggcccacg gtccggccac gcccgcccac
     3901 tcggccgcgt cgagcgacgc aagcagcggc tctggcagtc ccatctgggc catcagcccc
     3961 gcgccagccg ccgcgaagcc tgtgcccgac gccttgaggc gcgcctcgag cgcgcaccag
     4021 caccgcagga aggccgccgc tcgccgcggc ggatcgacgc cgagcagggc ccggacgacg
     4081 tccggatgga actgccggcg tgcgatctcc tcgatccgca cgtcccgccg cgcgccttcc
     4141 acatcgatgc ccaccgcgca ggctcgcgcg acggcgacga gcgcgacgcc gtccgagtgg
     4201 ctcaagttga agcggacggg cggggaggcg ttgccggcgt cgagttccgg tttgccggtc
     4261 gcgccgctta cgaacgccag cgacgccggt ccggcgcctt cgacgtaacg gctcagcacc
     4321 gtcctcagca tcgcgtgggc ggcgcagaat cggcgccggc ggcggggctg gatcagccgc
     4381 gcggcgcgtg cccgctccga gtcatccagg aacgccgcat ggaccgggtt cgtccatccc
     4441 ggttcgtcga ggtccgcgcg ccacacgtgc acgcgcccgt gcggaagcgc cgggcggcca
     4501 ggcgaggccg tccaccagga ggcggaaccg ttcatagctc cgcgccgcaa ggatccacgc
     4561 gaacgcgcga accgcccctc accgctctgc cgcgctggag ccgagcgccc ggcgcgacgg
     4621 ccgccatagc cgccggcgcg ccgaggggtg tcggcaggct gcggattggg cgacgaagga
     4681 ggttcggaac caccaggacg aaacagggca tcgcgggctg cacgcgactt gtaccgtgca
     4741 gggcgcgtcc gcgcaagcgg aatctccgcg tctccggccg cagcacgcgg ccgcgcccgc
     4801 gcgatgcgcc tcggccgcgg gatccggccg tccacgactg tctcgccgga aggcgagcgg
     4861 tcggtcgcgc cgatcgcgtg ctcgcggaga tcctgtcgcg cgtgatcgac gcggcggaat
     4921 cgcggcggtc cgcggcccac gagccgcgag atccttccgg gcagcggccc ttcggccccg
     4981 gcggtctcag agcgtcctga tcttgatgtt cctgaaccag accgcgtagc cgtggtcctg
     5041 caggccgatg tagccctcct tcgcgatccc ctcggtgaag cccgggaagg tcttgaactt
     5101 gctgttcgcg accatccggt cccactcggg cgtccacagc gtgtactcga ccaccttggt
     5161 gccgttctgg gtgtgcgtga ccttgccgtc cttcacgcgg accacgatcg tgttccactc
     5221 gccgacgggc ttgaccgtcg ccgggtcggc cgcgagcatg tcgtacagcg atccggcgag
     5281 gtggctggcg atcttgttgt cggtcgcgtc cacgttgtcg agcacctgca cct
//
LOCUS       DAOSIF010000176         5325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13813_length_5325_cov_4.161101, whole genome shotgun sequence.
ACCESSION   DAOSIF010000176 DAOSIF010000000
VERSION     DAOSIF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13813_length_5325_cov_4.161101"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLT35_07160"
     CDS             complement(<1..230)
                     /locus_tag="PLT35_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29044.1"
                     /translation="MRRTKRAIATDSSHRPRRIWQQRIKTSGHDRPLRPHVRQHCLTP
                     RGSDPVSWREAVLGAGCPSIFFETGEDDAAGCA"
     gene            complement(345..1163)
                     /locus_tag="PLT35_07165"
     CDS             complement(345..1163)
                     /locus_tag="PLT35_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29045.1"
                     /translation="MTNGAPQLIDVGAESVDRTGFFCCMSKKKSEGHQGKLRWLKERF
                     AEGMRIRLLAPPERGFIEYIPGEHAWRAVHADGYMLIHCLWVVGRSKGQGFSSTLLDA
                     CVADARKQRMKGVAMVTSERVWLAGRRVLDKHGFACVETAPPAFSLMVKQFGRHPPPS
                     FAGGWSDKAKAFGKGLTVIQSAQCPYTVDAANAAAAAAAKAGIESRVVELQSREDVMR
                     LSPSPYGVFGLVLDGRLLSYHYQLEKDLLPLLQGGALHELEGKTAKPDTPSTSR"
     gene            complement(1175..1435)
                     /locus_tag="PLT35_07170"
                     /pseudo
     CDS             complement(1175..1435)
                     /locus_tag="PLT35_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1648..1722)
                     /locus_tag="PLT35_07175"
     tRNA            complement(1648..1722)
                     /locus_tag="PLT35_07175"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1688..1690),aa:Asn,seq:gtt)
     gene            complement(1816..2025)
                     /locus_tag="PLT35_07180"
     CDS             complement(1816..2025)
                     /locus_tag="PLT35_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29046.1"
                     /translation="MDARELDEALRNLDRRTSRIEQILPTLSEGLEAVDRRDAAQHGG
                     LKGEIAHVIRRIVRLEAMPGLRQSR"
     gene            2250..2633
                     /locus_tag="PLT35_07185"
     CDS             2250..2633
                     /locus_tag="PLT35_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29047.1"
                     /translation="MKKVLVLAAWCVFLAALPVLAQEAAKPAVDVTGVWDSLVESPQG
                     AMVLVTTLRQEGDKVSGTQSTPMGELPLEGTVSGNDLTFTIKFDMQGQQAVVTFTAKV
                     ESDSMTGMYDFAGMGGAGWTAKKKQ"
     gene            complement(2642..4237)
                     /locus_tag="PLT35_07190"
     CDS             complement(2642..4237)
                     /locus_tag="PLT35_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG29048.1"
                     /translation="MPMTERPAGGPVWFRSLYWRIAIGVVALFAALLVAQALVFLWLS
                     GSIAANLMGSSPEELAAQVATETASALQQNPSLDLESFVRERYSRYYQPLMVVLEDGR
                     VARNHEPPAVPGRGPGLRGPRADGGRGGMADSADRGAGGPPAGGTRRPDAAAGEAPPP
                     GAGGSRFSRGGRVPPGSRPMAPLPGGQRASIEVAGSRVGFVAVAGRGPRTEFLLRQFG
                     PALAVIGTVLLLVGSAVSSLAIFRPARRRLRALETVARAIGEGNASARAPESGGDEVT
                     ALARAINQMASDLEARTSAMEASDRTRRQLLADVSHELKTPLAAIRGYVETLAMPEVE
                     LDADIRRRYLDIVGEETIKLEGIVGDLLDLARLEGGGMTLTCESVPVAALFQRVNDRH
                     ERGLLEKQINVETAIAPDAGEVWADPARIEQALQNLVSNALRHVPEGGQITLASERAG
                     GRVRLVVRDSGPGIPEDHLPRIFDRFYKTDASRSDPYSKSGSGLGLSIVKAIVELHGG
                     SAAAANLAGGGAEFAIALPPRPA"
     gene            complement(4224..4961)
                     /locus_tag="PLT35_07195"
     CDS             complement(4224..4961)
                     /locus_tag="PLT35_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG29049.1"
                     /translation="MERERATPAAGRALIVEDEPRIRELVALHLSLEGLACVEAGTGD
                     EALRLARAERFDLIVLDLMLPGLDGVTVCRAIRRDSPNAATPILMLTARSEESDKVIG
                     LESGADDYLTKPFGVRELVARARALLRRAGAARAAHAPDAAGIVSAAGLAIDPSRRQV
                     RAGERAIDLTALEFELLHLLASRRGLVFSRETLLARVWKDDRHVTERSVDTLVKRVRK
                     KIEPDPENPRYVLTVWGAGYKFADADD"
BASE COUNT          767 a         1963 c         1815 g          780 t
ORIGIN      
        1 gcgcagccgg cggcgtcgtc ctcgccggtc tcgaagaaaa tcgaggggca acccgcgccc
       61 aggacggctt cccgccagga aaccggatcg gaccctcgcg gcgtcaggca gtgctggcgg
      121 acgtgcggcc tcaagggtcg gtcgtgcccg gaggtcttga tccgctgctg ccagatccgc
      181 ctcgggcggt gagacgagtc cgtcgcgatg gctcgcttcg ttcttctcac gcctgttggc
      241 ctgctctcgg gccgcgtgga tgcgtcggcg agacgacctg gcggcttcat gcctgaccag
      301 cgtgtctctc cggccaagac cccaatcgag cgcgccgcgt cttctcacct cgaggtcgat
      361 ggggtatcgg gtttcgctgt cttcccttcg agctcgtgca gggcgcctcc ttgaaggaga
      421 ggcagcaggt ccttttcgag ctggtagtgg tagctgagga gcctgccgtc gaggacgagg
      481 ccaaagacgc cgtacggtga tggcgacagc cgcatgacgt cctcgcggct ctgcaactcg
      541 accacccggc tctcgatccc cgccttggca gccgcggccg cggcggcgtt ggccgcatcg
      601 acggtgtacg ggcactgggc cgactgaatg acggtcagac ccttgccgaa tgccttcgcc
      661 ttgtcgctcc agccaccggc gaacgacggc ggcgggtgcc tgccgaactg cttcaccatc
      721 agggagaagg ccggcggcgc ggtctcgacg cacgcgaacc catgcttgtc cagcacgcgg
      781 cggccagcca gccacaccct ctcgctggtc accatggcga cgcctttcat ccgctgcttc
      841 ctggcgtcgg cgacgcaggc atcgagcagc gtgctcgaga acccctggcc cttgctcctg
      901 ccgacgaccc acaggcaatg aatgagcatg tatccgtcgg catgaaccgc gcgccaggcg
      961 tgctcgccgg ggatgtactc gatgaagccg cgctcgggcg gtgcgagcag cctgatgcgc
     1021 atgccctcgg cgaagcgctc cttcagccag cggagcttgc cttgatggcc ctcggacttc
     1081 ttcttgctca tgcagcagaa gaagccggtc ctgtcgacgc tctcggcgcc gacatcgatc
     1141 agctggggcg cgccgttcgt caatcgtacc ggcttcagcg gagtgcgggt cgtgcgagtc
     1201 ggcccgcctt ggcctgctca tgatagcgct cttcgtgctc cgccggcggt acgtggccga
     1261 gcggctcaag tagccgccgc atgtcgaccc ggtgcaccgg gaccggcgcg gcagactcga
     1321 ccatctccgg actgcgccac gggccccggc gttgaatgac ctcggtcttg aaggggccga
     1381 tcaccgtctc cgcgagcgta gtgtcgcagg ctccgccggt gccgcccacc gatggcgcgc
     1441 tcacgcgcct cagggaaaga ccgcttcgag agtcgcatag ggcccatcct ctctctgagc
     1501 ggaccctccg acaacccggc tccgggccgt cgaccttcca cgcggtgagc gtggctcccc
     1561 atccgccctc gccccgagcg aagtcgaggg gctacggcgt gatttcgtcg ccaatccgtc
     1621 tgggcaaagc ggagtgtcgg ggaggactgg ctcctcaggt aggactcgaa cctacaaccc
     1681 tccggttaac agccggatgc tctgccattg agctactgag gaacgctggc agacgctgcg
     1741 gccgctgggg ccggccgcaa aggtcgatga tagccggatt gcaggaaccc ggtcaatccg
     1801 ccggcgcgcg cccgctcaac gcgactgccg cagcccgggc atggcctcca gccgcacgat
     1861 gcgccggatg acatgggcga tctcgccctt cagcccgccg tgctgcgcgg cgtcgcgcct
     1921 gtccacggcc tcgaggccct ctgagagagt tggcagaatc tgctcgatcc gggacgtccg
     1981 ccggtcgagg tttctgagtg cttcgtcgag ttcgcgcgcg tccacccctc cgctccgatc
     2041 ccgacctcct gcgtgaaaac cccacaatga ccggacgccg cgcgcgcctg tcgcgcgacc
     2101 gcccgtgcgg catccggccc aggccctgca aggctggtgc ctggtccgcg cctcacggcc
     2161 gctgacggca gtgcacggcg ttcttgaccg gctccccctc ccccgatact ctcttgcgcg
     2221 accggccgca cgccggaagg agcgatcgaa tgaagaaggt cttggtactc gccgcctggt
     2281 gcgtcttcct cgccgcgctc ccggttcttg cgcaggaggc cgcgaaaccc gcagtagacg
     2341 tcacgggcgt ctgggactcg ttggttgaat cgcctcaagg cgcgatggtg ctcgtgacga
     2401 ccctcaggca ggaaggcgac aaggtgagcg gcacgcagtc caccccgatg ggcgaactgc
     2461 ccctcgaggg cacggtttcc gggaacgacc tcacgttcac gatcaagttc gacatgcagg
     2521 gccagcaggc cgtcgtcacc ttcaccgcga aggtcgagag cgacagcatg acgggcatgt
     2581 acgacttcgc cgggatgggc ggggccggct ggacggcgaa gaagaagcag taggccgccg
     2641 gctacgccgg acgcggcggc agggcgatcg cgaattccgc gccgccgccg gcgaggttcg
     2701 ccgccgcggc gctcccgccg tgaagctcca cgatggcctt cacgatggac aggccgagcc
     2761 cgctcccgct cttggagtac ggatcgcttc gggacgcgtc ggtcttgtag aagcgatcga
     2821 agatgcgcgg caggtggtct tcggggatcc cgggcccgct gtcgcggaca accagccgca
     2881 cccggccccc ggcgcgctcc gacgccaggg tgatctgccc gccctccggg acgtgacgca
     2941 gcgcgttgga cacgaggttc tgcagcgcct gctcgatccg ggcgggatcg gcccacacct
     3001 cgccggcgtc tggagcaatg gcggtctcca cgttgatctg cttctcgagc agcccgcgct
     3061 cgtgcctgtc gttgacgcgc tggaacagcg ccgcgacggg cacggactcg cacgtcagcg
     3121 tcatcccgcc gccctcgagg cgcgccaggt ccagcaagtc cccgacgatc ccctcgagct
     3181 tgatcgtctc ctcgcccacg atgtccaggt agcgccggcg gatgtccgca tcgagctcca
     3241 cctcgggcat cgccagcgtc tcgacgtacc cgcgaatggc ggcgagcggc gtcttcaact
     3301 cgtgcgacac gtcggcgagc agctgccggc gggtccggtc cgacgcttcc atggcggacg
     3361 tgcgcgcctc gaggtcggac gccatctggt tgatggcacg ggccagcgcc gtgacctcgt
     3421 cgccgcccga ctccggggcc cgcgccgagg cgttcccctc gccaatcgcg cgcgcgacgg
     3481 tctcgagcgc gcggagccgc cggcgcgcgg gcctgaagat cgccagcgag ctgacagccg
     3541 agccgacaag cagcagtacc gtgccgatga cggcgagcgc ggggccgaac tgccggagca
     3601 ggaactcggt ccgcgggccg cgcccggcaa ccgccacgaa cccgacgcgg ctgcccgcca
     3661 cctcgatgga cgcacgctgg ccgcctggca gcggcgccat cggccgcgac cctggcggca
     3721 cgcggccgcc ccgcgagaag cggctgccgc ccgcccccgg cggcggcgcc tcccccgccg
     3781 cggcgtccgg ccgccgcgtc ccgcccgcag gcggcccgcc cgctccgcga tcggccgagt
     3841 ccgccatgcc gccccgcccg ccgtccgccc tcgggccgcg cagcccgggc ccgcggccgg
     3901 gcacggccgg cggctcgtgg ttgcgcgcga cccgcccgtc ttccagcacc accatgagcg
     3961 gctggtaata gcggctatat cgctcgcgga cgaagctctc gagatcgagc gaggggttct
     4021 gctgcagggc cgaggccgtc tccgtcgcca cctgggccgc gagctcctcc ggcgacgatc
     4081 ccatcaggtt cgcggcgatg ctcccgctca gccagaggaa gacgagcgcc tgcgccacga
     4141 gcagcgctgc gaacagcgcg accacgccga tggcgatgcg ccagtagagg ctgcgaaacc
     4201 agacggggcc gccggcaggg cgctcagtca tcggcatcgg cgaacttgta ccccgcgccc
     4261 cagacggtca ggacgtagcg ggggttctcg ggatcgggct cgatcttctt gcggacgcgc
     4321 ttcaccagcg tgtcgacgct ccgctcggtg acgtggcggt cgtccttcca cacgcgcgcg
     4381 agcagggtct cgcgcgagaa cacgaggccg cgcctcgatg cgagcaggtg cagcagctcg
     4441 aactcgaggg cggtcagatc gatcgcgcgc tccccggcgc gcacctggcg gcgcgacgga
     4501 tcgatggcca ggcccgcggc agacacgatt ccggcggcgt cgggagcgtg cgccgcccgc
     4561 gcggctcccg cccggcgcag cagggcgcgc gcccgggcca ccagctccct gacgccgaac
     4621 ggcttggtca gatagtcgtc ggccccgctc tcgaggccga tcaccttgtc ggactcctcg
     4681 gaacgagccg tgagcatcag gatcggcgtc gccgcgttcg gcgagtcccg ccggatcgcg
     4741 cggcacacgg tgacgccgtc caggcccggc agcatgagat cgagcacgat caggtcgaac
     4801 cgctccgcgc gcgcgaggcg gagggcctcg tcgccggtgc cggcctcgac gcaggccagc
     4861 ccctccagcg acaggtgcag ggcgacgagt tcgcggatcc gcggctcgtc ctcgacgatc
     4921 agcgcccggc cggcggcagg cgtcgcgcgt tcgcgttcca tgtctcctcg cctccgcatt
     4981 gtagaccgct atgcaggacg gcgagcgcgc cgagagggcg tgtcgcagaa gaatcaccgg
     5041 cgtgccacgc cgccgccatg gcgcggccgg gacgcccggc gacaacggca cccggccgca
     5101 tcggctgccg cagcggctcc agcgtgccgc gccatcctgc ccgtccgcgg ctaagcgcga
     5161 ggccgccggc cgtcgtagat gacagagggt cgctgccttc ccgacccgga gcggagcccc
     5221 atgtcctcga cgcctgacgg cacggtccgt gcccggacgc gccaagctgc tctccggccc
     5281 gcggccccga tcctgctggc agccttgaca gccgctgcct cggcg
//
LOCUS       DAOSIF010000177         5322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13829_length_5322_cov_3.615910, whole genome shotgun sequence.
ACCESSION   DAOSIF010000177 DAOSIF010000000
VERSION     DAOSIF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13829_length_5322_cov_3.615910"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /gene="speA"
                     /locus_tag="PLT35_07200"
     CDS             <1..1503
                     /gene="speA"
                     /locus_tag="PLT35_07200"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOG29050.1"
                     /translation="SDLVALASNETPIVCNGFKDAEFIETAMLAQKVGRTVLPVVEKY
                     SELGLILECARQVGVRPTIGMRVKLAARGSGKWQSSGGYRSKFGLTVSEVLKGLEELK
                     ALGMQDCFKLLHFHLGSQIPNIRVVKAAVNEAARIYVELAKAGAGLEYLDIGGGLGVD
                     YDGSRTNFESSVNYTLEEYAADVVYHVQTVCDDAGVKHPTIVSESGRAIAAYHSVLVF
                     NVLGVTGFREQEIRAVSPHDEQEQPLIDLRETYQSVSIRNALESYHDAQQALDMATNL
                     FNAGYLPLDQRCEAETLYWAICARLQRLIQQVDEIPEDLQSLDEMLADTYFCNFSLFQ
                     SAPDSWAIKQLFPVMPIHRLDTPPTNHAILGDITCDSDGKIDQFIDRRDVKRTLPLHP
                     ANGEPYYLGIFLIGAYQEILGDMHNLFGDTHAVHVSLDEKGGVVLDAVIKGDTVREVL
                     QYVDFDTNALEAKLRADAEAAVREGRMSFEESGRLLRFYESGLGGYTYLE"
     gene            complement(1888..3720)
                     /locus_tag="PLT35_07205"
     CDS             complement(1888..3720)
                     /locus_tag="PLT35_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG29051.1"
                     /translation="MRTGRLAGAAAAALAAAISLQAGHGQAPDQYGAATPADLSSLAP
                     ASAARSLDELAARFDQRAAIDLVRFMDGYWREAANAGFNATLDRIKARLEASGFAPAD
                     TAPAGAPTVRVESFGRANGWNHTVGTLALAAEGERPEEILLSRERHRVALCINSFSTP
                     PGGIVAPVVDVGEGASDADYEGRDVRGAVVLGDSGAFRLWQAAVVRRGAIGIVSTALD
                     GYIEPGPSAADRAKPRREWDVLQWGSVPYDETRRGFGFKATPRAAALIRERLKAGPAR
                     VRVEVASTFTPGPIRTLVAEIPGAIAPQERVVMVAHVQEPGANDNASGCASLQELARA
                     IAAGMRQRTIAAPGRTLTFLWGDEIRASRQWIVDHPVEAKQVRYMIALDMTGEDVTKT
                     GGSFLIEKAPDPAAVWDRPSDPHTDWGGGHVKAGSLRGTLLNDLFLAICQVRAQRTGW
                     VVRTNPYEGGSDHTPFVASGVPALLAWHFPDRFYHTNLDRPEMTSPSEMEHVAVSAGA
                     AALLLAGASATEARQVVALLEHAARERLALERRQGAALVRAAPDRAAAEATEAEVMAA
                     WLTWYAEAVRSVADLPPGGADAALARRIDEAVTRLGGRGASKPG"
     gene            complement(3720..>5322)
                     /locus_tag="PLT35_07210"
     CDS             complement(3720..>5322)
                     /locus_tag="PLT35_07210"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOG29052.1"
                     /translation="TIQPRFTGTWNGIFRTIPVEYRTPQGFSYDLDLDIESVTGGNGS
                     ALRFESSRERHYRKLKIWVPGAQDAVKTVVITYRVRHGLKFFEEHDELYWNVTGDEWE
                     VPIERARAVVYLPAGVTGLRAYAFTGGYGSQEAAARTTIVGTNITVETTRVLNFREGV
                     TVAVAWNPGVVARPTNAEKAAGFAFANLPLLIPVGVFGLMFGVWKRFGRDPKTLPVTV
                     QYEPPGGMTPAEAGTLVDNRPDMRDITASIVDLAVRGFLRIEETTEPRFLGFGKRTGY
                     LLKLLKPSTGWKGLRTHEEKLLHALFLRTERPGSSQPDASGVVDEVAISDLEDRFYTN
                     LPEIRERIFSQLIDRGYYRRRPDKARAPFIIGGFASLLAGGWVCAALGAGTAGWLATV
                     ASAVIIFAFGLVMPARTVRGARAREGVLGFEEFLKRVDADKFERVARTPDMFERFLPY
                     AMALGVERNWARAFEGIYTSQPDWYVGGTPGVFGTRMFASSLANLSSTAGSAMASSPR
                     SSGGSGTGGGGFSGGGFGGGGGGGF"
BASE COUNT          806 a         1924 c         1836 g          756 t
ORIGIN      
        1 tccgatctgg tcgccctcgc gtcgaacgag acgcccatcg tctgcaacgg cttcaaggac
       61 gccgagttca tcgagaccgc gatgctcgcg cagaaggtgg gccgcacggt gctgcccgtc
      121 gtcgagaagt acagcgagct ggggctgatc ctggaatgcg cgcggcaggt gggcgtccgg
      181 ccgaccatcg ggatgcgcgt caagctcgcg gcgcgcggca gcgggaagtg gcagtcctcg
      241 ggcgggtacc gctccaagtt cggcctgacg gtgtcggagg tgctcaaggg gctcgaggag
      301 ctgaaggccc tgggcatgca ggactgcttc aagctcctgc acttccacct cggcagccag
      361 atcccgaaca tccgcgtggt gaaggcggcg gtcaacgagg ccgcccggat ctacgtcgag
      421 ctggcgaagg cgggcgcggg cctggagtac ctcgacatcg gcggcggcct cggcgtggac
      481 tacgacggct cccggaccaa cttcgagtcg agcgtgaact acacgctcga ggagtacgcc
      541 gccgacgtcg tctatcacgt gcagacggtg tgcgacgatg ccggcgtgaa gcacccgacg
      601 atcgtgtcgg agagcgggcg cgcgatcgcg gcctaccaca gcgtcctcgt tttcaacgtc
      661 ctcggagtca ccggcttccg cgagcaggag atccgggccg tcagcccgca tgacgagcag
      721 gagcagccgc tcatcgacct gcgcgagacc tatcagagcg tgtcgatccg gaacgccctc
      781 gagagctacc acgacgcgca gcaggcgctc gacatggcga ccaacctgtt caacgcgggc
      841 tacctgccgc tcgatcagcg gtgcgaggcc gagacgctgt actgggccat ctgcgcgcgg
      901 ctccagcgcc tgatccagca ggtcgacgag attccggagg acttgcagag cctcgacgag
      961 atgctggcgg acacgtactt ctgcaacttc tcgctgttcc agtcggcgcc ggacagctgg
     1021 gccatcaagc agctcttccc ggtgatgccg attcaccgcc tggacacgcc gccgacgaac
     1081 cacgccatcc tcggcgacat cacgtgcgac tcggacggca agatcgacca gttcatcgac
     1141 cgccgcgacg tcaagcgcac gctgcccctc catcccgcca acggcgagcc ctactacctc
     1201 gggatcttcc tcatcggcgc ctaccaggaa atcctcggcg acatgcacaa cctcttcggc
     1261 gacacccacg ctgtgcacgt cagcctggac gagaagggcg gcgtcgtgct cgacgcggtc
     1321 atcaagggcg acacggtgcg cgaggtcctg cagtacgtgg atttcgacac caacgcgctc
     1381 gaggccaagc tgcgcgcgga cgccgaggcg gcggtgcgcg agggccgcat gtcgttcgag
     1441 gagtcgggcc gcctgctcag gttctacgaa agcgggctgg ggggctacac ctacctcgag
     1501 tagggcccgg gcgccggctc gctgggcgcc cccggtcgat gcccggcctc aagactgcct
     1561 cgcaacaggc gccgcggccc gatcggcgcc agtcgcctgc tcctcgaagc ccgctgcccg
     1621 cgcccttcct cgcgtcgcgt cacgtccgcc gacagcaggc gggcggccgc ccgcgcaggg
     1681 tgccacccgg acaccgcacc aatcacgacg gcggcgcccg ccgtcaccgc gagcatcagc
     1741 acgctgatgg cggcatcgag ccgcgcggcg gtctggcggc gcatgatgac cgccgccgag
     1801 gaggcccctg acggtgaacc gccgcgtcgg cagcggtcgt cggtgcagga ggacagggat
     1861 ccgcgggccg aggggcgccg ctcggggcta tccgggcttc gacgcgccgc ggccgcccag
     1921 gcgcgtgacg gcctcgtcta tccggcgcgc cagcgcggcg tccgcgccac cgggagggag
     1981 atccgcgacg ctgcgcaccg cctcggcgta ccaggtcagc caagcggcca tcacctcggc
     2041 ttccgtggct tctgccgcgg cccggtcggg tgcggcccga accagcgccg cgccctgccg
     2101 ccgctcgagc gccagccgct cgcgcgccgc atgctcgagg agcgcgacca cctgccgcgc
     2161 ctccgtggcc gaggcgcccg cgagcagcaa cgccgcggcg ccggcgctca cggcgacgtg
     2221 ctccatctcg gacgggctgg tcatctcggg ccgatccagg ttcgtgtgat agaagcgatc
     2281 ggggaagtgc caggccagca gcgcgggtac gccggaggcg acaaacggcg tgtggtcgct
     2341 gcctccctca tagggattcg tccggaccac ccagccggtg cgttgcgccc ggacctggca
     2401 gatggccagg aacaggtcgt tgagcagcgt gccccgcagc gagccggcct tgacgtggcc
     2461 gcctccccag tcggtgtgcg gatcggacgg acggtcccag accgctgcag gatccggcgc
     2521 cttctcgatg aggaacgacc cgccggtctt cgtcacgtct tcaccggtca tgtcgagcgc
     2581 gatcatgtac cggacctgct tcgcctcgac ggggtgatcg acgatccact gccggctcgc
     2641 gcggatctcg tcgccccaga ggaacgtcag cgtgcggcca ggcgcggcga tcgtccgctg
     2701 gcgcatcccg gcggcgatgg cccgggcgag ttcctgcagc gaggcgcacc cgctggcgtt
     2761 gtcgttcgct cccggttcct ggacgtgcgc caccatcacc acgcgctcct gtggggcgat
     2821 ggcgccgggg atctcggcga cgagcgtccg gattggcccc ggcgtgaacg tcgaggcgac
     2881 ctcgacgcgg acccgcgccg ggccggcctt caggcgctcc cggatgaggg cggccgcccg
     2941 cggcgtggct ttgaacccga acccccgccg ggtctcgtcg tagggcacgc tgccccactg
     3001 gagcacgtcc cactcgcggc gcggcttcgc gcgatcggcg gctgaggggc ccggttcgat
     3061 gtagccgtcg agcgccgtcg agacgatgcc gatcgcgccg cgccggacga cagccgcctg
     3121 ccagagccgg aacgcgcccg aatccccgag cacgacggcc ccgcggacgt cccgaccctc
     3181 gtagtccgcg tcggacgccc cctcgccgac atcaaccacg ggtgcgacga tgccgcctgg
     3241 cggagtggaa aacgagttga tgcagagcgc gacgcggtgg cgctcacgcg acagcaggat
     3301 ctcctccgga cgctcgcctt cggcagccag cgcgagcgtg ccgaccgtat gattccagcc
     3361 gttcgcccgg ccgaacgact ccacgcgcac ggtgggcgca cccgcgggcg ccgtgtcggc
     3421 cggagcgaag cccgaggcct cgaggcgagc cttgatgcgg tcgagggttg cgttgaagcc
     3481 ggcgttggcc gcctcgcgcc agtagccgtc catgaaccgg accaggtcga tcgcggcgcg
     3541 ctggtcgaag cgggccgcga gttcgtcgag gctccgcgct gccgaggccg gcgccagcga
     3601 ggacagatcg gccggcgtgg ctgcgccgta ctggtctggc gcctgtccgt gccctgcctg
     3661 caggctgatt gccgccgcca gcgcggcggc cgccgcgccc gccaaccgcc ccgtgcgcat
     3721 cagaagcctc cccctcctcc gccgccgaag ccgccgcccg agaagccgcc ccctccagta
     3781 ccggacccgc ccgagctcct cggcgaggac gccatggcgg aacccgccgt gctcgacaga
     3841 ttcgccagcg acgaggcgaa catccgcgtg ccgaacacgc cgggcgtccc gcccacgtac
     3901 cagtccggct ggctcgtgta gatgccctcg aacgccctcg cccagttgcg ctcgacgccg
     3961 agggccatcg cgtacggcag gaaccgctcg aacatgtccg gtgtgcgcgc cacgcgctcg
     4021 aacttgtcgg cgtcgacgcg cttgaggaac tcctcgaatc ccagcacgcc ctcgcgggcc
     4081 cgggcgcccc tgacggtcct cgccggcatg acgaggccga aggcgaagat gatgacggcc
     4141 gacgccaccg tggccagcca gccggccgtg ccggcgccga gggccgcgca gacccagccg
     4201 cccgccagca gggaggcgaa tccgccgatg atgaacggcg ctctggcctt gtcggggcgc
     4261 ctccggtagt acccgcgatc gatgagctgg ctgaagatgc gctcgcggat ctccggcagg
     4321 ttcgtgtaga acctgtcctc gagatccgag atcgcgactt cgtcgacgac gccggaggca
     4381 tcgggctggc tcgagcccgg cctctcggtc cggaggaaaa gcgcgtgcag cagcttctcc
     4441 tcgtgcgtcc ggaggccctt ccagccggtt gacggcttga gcagcttgag caggtagccg
     4501 gtccgcttgc cgaaccccag gaagcgcggc tccgttgtct cctcgatccg gaggaagccc
     4561 cgcaccgcca gatcgacaat cgacgcggtg atgtcccgca tgtcggggcg gttgtcgacg
     4621 agcgtccccg cctcggcggg cgtcatgccg cccggcggct cgtactgcac cgtgaccggc
     4681 agcgttttcg ggtccctgcc gaagcgcttc cacacaccga acatcaggcc gaacacgccg
     4741 accggaatga gcagtgggag atttgcgaat gcgaagcccg cggccttctc cgcgttggtc
     4801 ggccgcgcaa ccacgcctgg gttccacgcg acggcgaccg tgacgccctc acggaagttc
     4861 aggacgcggg tcgtctcgac ggtaatgttc gtgccgacga tcgtcgtccg cgcggcggcc
     4921 tcctgcgatc cgtagccgcc ggtgaacgcg tacgcgcgca ggcccgtcac gcccgccggc
     4981 aggtagacga cggcgcgggc gcgctcgatg gggacctccc attcgtcgcc cgtcacgttc
     5041 cagtacagct cgtcgtgctc ctcgaagaac ttgaggccgt gacgcacgcg gtacgtgatg
     5101 acgacggtct tcacggcgtc ctgcgcgcca ggcacccaga tcttgagctt ccggtagtgc
     5161 cgctcgcggc tcgattcgaa gcgcagcgcg gagccgttcc cgccggtgac ggactcgatg
     5221 tccaggtcga ggtcgtacga gaacccctgc ggcgtgcggt actcgacggg aatggtccgg
     5281 aagatgccgt tccacgtgcc ggtgaagcgc ggctggatcg tc
//
LOCUS       DAOSIF010000178         5296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13920_length_5296_cov_4.065446, whole genome shotgun sequence.
ACCESSION   DAOSIF010000178 DAOSIF010000000
VERSION     DAOSIF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13920_length_5296_cov_4.065446"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="PLT35_07215"
     CDS             complement(<1..1809)
                     /locus_tag="PLT35_07215"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29053.1"
                     /translation="MRLSLKARQVAAVTSIVGVAVIVLGAVYVTGVARVRLEETRARG
                     VFLANAIYHRARDVVATSAQPYAALRSDPGLRSILESSVYSENVTYAAIVDASEVVVA
                     HSDSACVGQTMPARPGLDALLADRPWAQVRAIYADAGRMLEIQQPLLMGGSRFGTIRV
                     GVSMLLARADLGRAMRPAVLTALAALGLSSLIAMILAQSLLRPIHLIRSGLSRLGRGE
                     FGVKLDLPREDELGELGGFFNTVSERISADRSKLEGETSRLESIVGRLEDAVAFFAPG
                     GELLFGNPAMSAALPAGLAGRTLAEIWPDGDPCRSLVEQTASDGQSRGPVAIDAGAPG
                     GGPRQADGSAGRGRRLVMTHAIEDGAGRTLGVMLVSRDLGYIGEVESTLQYSRKVAAL
                     GRLTAGVAHEIKNSLHAATIHLELVKQDLGAGGPRAQPVDREAAEAHVASIAATLKRL
                     DEMLQGLLTFTRPEELKLQEVGLAAVVGSLMPIVRAEAGKAGVEIAVDCPEDLPMLRA
                     DPGALQQALLNLALNACQAMPGGGRLRMAGRALRDRRVEIVVEDTGAGIPPEHIGQIF
                     NLYFTTRQGGTGIGLSMVYRTIQLHDGDIEVQSSPGR"
     gene            complement(1813..3207)
                     /locus_tag="PLT35_07220"
     CDS             complement(1813..3207)
                     /locus_tag="PLT35_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG29054.1"
                     /translation="MPPDTAPDDRVRILLVEDDAASRVGFQQLLQGWGFAVEAACDGE
                     DALAKAPAFRPDIVLTDLVMPRLDGLGLLRALQQEDGEITTVILTAQGTVDTAVEALK
                     HGAYDYLSKPVDIQRLRVVLDKIIERQETLREVKALRRQLREHGSFGRMIGRSPAMQA
                     VYRVIEQAAPTSASVLVSGESGTGKELVAQTIHRLSPRASAPWVPINCAAIPEALLES
                     ELFGHERGAFTGAVERQAGCFELAHRGTLFLDEIAEMAPAMQVKLLRVLQERSFRRLG
                     GRVEQSVDVRVIAATNSDPASAVETGRLREDLYYRLNVVAIRLPPLRERMEDLPILVD
                     SFVREFGELHAKSAAGISPAARRALESYAWPGNVRELRNVIERATIMASGRLVEAQDL
                     PPLGRPSSQPPQGGGPLQPGMTVDEAERRLIMLTLEHTGNNKTRAAEMLGISLKTLHN
                     KLNRFRDLGLGSGA"
     gene            3311..4282
                     /locus_tag="PLT35_07225"
     CDS             3311..4282
                     /locus_tag="PLT35_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29055.1"
                     /translation="MHKKTALVVLVLLAATLPTACAGPDDRAGPAAARSQTGPSASAV
                     EGGGGGASPLGRLYGATGAARNLGAVAFPPRNEPYAFRQELEAKYRDGLGRPPSPTFV
                     DLEGDIVWTQEYVRYRVNGCDHPAAAQRVFDQIDGRGVSPVCGDAAPGPIAFPPRNEP
                     YAFRLELEAKYRDGLHRPATSSTVDIEGSIVWTQEYLRYRVNRCGHAAAAAKVFSQID
                     GQGIAPTCWDALSGVWDGESDYFNAPFTMDVYMRGASILGRYSDRHDTGSLWGTYESG
                     PAVVINAYFGDGGIRFDGAFAGPDTIRGSMRVSTLSRLYHFTMTRRP"
     gene            4337..>5296
                     /locus_tag="PLT35_07230"
     CDS             4337..>5296
                     /locus_tag="PLT35_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOG29056.1"
                     /translation="MSRLLSVAVWAAVAAAGAAAVLVSALHRGESINALWIVTAGLCS
                     FAIAYRFYSKWLAVKVLAIDDRRAPPSITERDGKDFVPTNTWVVYGHHFAAIAGPGPL
                     VGPVLAAQFGYLPGMLWILVGATLGGGVHDAVILFASMRRCGRSLGQMLKEEVNPVVG
                     VIALVSLLAIMTILLAVLGLVVVKALAESPWGLFTIAMTIPLALAMGLAHTVGRVSVR
                     TVTVFGVTALLASVWGGSQLGAWGLTPWFDRGAEWIAWAIILYGFAASVLPVWLLLAP
                     RDYLSTYMKLGTVAVLAAAIVIVAPPLHMPALTRYVGGTGPVFA"
BASE COUNT          705 a         1910 c         1904 g          777 t
ORIGIN      
        1 gcggcctggc gacgactgca cctcgatgtc accgtcgtgc agctgaatgg tccggtacac
       61 catcgacagg ccgatcccgg ttccgccctg gcgggtggtg aaatacaggt tgaagatctg
      121 gccgatgtgc tccggcggga tgcccgcgcc ggtgtcctcg acgacgatct cgacgcgccg
      181 gtcgcggagg gcgcgcccgg ccatcctgag ccgcccgccg cccggcatgg cctggcaggc
      241 gttcagggcc aggttcagca gcgcctgctg cagcgcgccg gggtcggccc gcagcatcgg
      301 caggtcctcc gggcagtcca ccgcgatctc gacgccggcc tttcctgcct cggcccggac
      361 gatgggcatc agcgaaccca cgacggccgc cagtcccacc tcctgcagct tcagctcctc
      421 tggccgcgtg aaggtcagca ggccctgcag catttcgtcc agccgcttga gggtggcggc
      481 gatggacgcg acgtgcgcct cggccgcctc ccggtcgacc ggctgcgcgc gtggcccgcc
      541 cgcgccgaga tcctgcttca cgagctcgag gtggatcgtc gccgcgtgca gcgagttctt
      601 gatctcgtgc gcgacgccgg cggtcaggcg gccgagcgcc gccaccttcc gcgaatactg
      661 cagcgtcgat tccacctcgc cgatgtagcc gaggtcgcgc gacacgagca tcacgccgag
      721 ggtgcgcccc gcgccgtcct cgatggcgtg cgtcatgacg aggcgccggc cgcggccggc
      781 gctcccgtct gcctggcgag ggccgcctcc cggggcgccg gcgtcaatgg cgacgggccc
      841 ccgcgactgc ccgtcggagg cggtctgttc caccaggctc cggcagggat cgccgtccgg
      901 ccagatctcg gcgagcgtcc ggcccgcgag cccggccggg agcgccgccg acatggcggg
      961 gttgccgaag agcagctcgc cgcccggcgc gaagaacgcg accgcgtcct cgagccggcc
     1021 gacgatggac tcgagcctcg acgtctcgcc ctcgagtttc gatcggtcag cggagatccg
     1081 ctcgctcacc gtgttgaaga agccgcccag ctcgccgagt tcgtcctcac gcggcaggtc
     1141 gagcttcacg ccgaactcgc cgcgcccgag gcgcgacagg ccgcttctaa tgaggtggat
     1201 gggccgcagc agggactgcg ccagaatcat cgcgatcagg ctcgacagcc cgagggcggc
     1261 aagcgccgtg agcaccgcgg ggcgcatggc gcggccgaga tccgcccggg ccaggagcat
     1321 cgacacgccc acgcggatcg tgccgaacct cgatccgccc atcagcagcg gctgctggat
     1381 ctcgagcatc cgcccggcgt cggcgtagat cgcgcgcacc tgcgcccacg gccggtccgc
     1441 cagcagcgcg tccaggcccg gccgcgccgg catggtctgt cccacgcacg ccgagtcgct
     1501 gtgggcgacg acgacctcgc tggcgtcgac gatggcggcg taggtgacgt tctcggagta
     1561 gacgctggac tcgaggatcg agcggagccc ggggtccgat cgcaaggcgg cgtacggctg
     1621 ggcggacgtc gcgacgacgt cccgggcgcg gtggtagatc gcattcgcca ggaacacgcc
     1681 gcgggcccgg gtctcctcca ggcgcacgcg cgcgacgccc gtgacataga cggcgcccag
     1741 cacgatcacg gcgacgccga cgatggacgt gacggcggcg acctgccggg ccttgaggct
     1801 gaggcgcatg gcctaggcgc cgctccccag gccgaggtcg cggaagcggt tcagcttgtt
     1861 gtgcagcgtc ttgaggctga tgccgagcat ctcggccgcc cgggtcttgt tgttcccggt
     1921 gtgctcgagg gtcagcatga tcagccgccg ctcggcctcg tccacggtca tgccgggctg
     1981 gagcggtccg ccgccctgcg gaggctgcga agacggccgg cccaggggcg gcaggtcctg
     2041 cgcctcgacc aggcggccgg aggccatgat tgtcgcccgc tcgatgacgt tccgcagttc
     2101 gcgcacgttg ccgggccacg cgtacgactc gagcgcgcga cgggcggccg gcgagatgcc
     2161 cgcggccgac ttcgcgtgca gttcgccgaa ctcccgcacg aagctgtcca ccaggatcgg
     2221 caggtcctcc atgcgctcgc gcagcggcgg cagcctgatc gcgacgacgt tgaggcggta
     2281 gtacagatcc tcgcggagcc gccccgtctc gacggcagat gcgggatcgc tgttcgtcgc
     2341 cgcgatcacg cggacgtcca cgctctgctc gacgcgcccg ccgaggcggc ggaacgaccg
     2401 ctcctgcagg acgcggagca gcttcacctg catcgccggg gccatctcgg caatctcgtc
     2461 gagaaacagc gtgccgcggt gggccagctc gaagcagccg gcctggcgct cgaccgcgcc
     2521 ggtgaacgcg ccccgctcgt ggccgaagag ctccgactcg aggagcgcct ccgggatcgc
     2581 ggcgcagttg atcggcaccc acggggccga cgcccggggg ctcaggcggt ggatggtctg
     2641 cgcaaccagc tccttgcccg tgccggactc gccgctgacg agcaccgacg cgctggtcgg
     2701 cgcggcctgc tcgatcacgc ggtagacggc ctgcatggcg gggctccggc cgatcatgcg
     2761 gccgaaggag ccgtgctcgc gcagctggcg gcgcagcgcc ttcacctcgc gcagggtctc
     2821 ctggcgctcg atgatcttgt cgagcaccac gcgcaggcgc tggatgtcca ccggcttgct
     2881 caggtagtcg tacgccccgt gcttgagggc ctcgacggcc gtgtccaccg tcccctgcgc
     2941 ggtgaggatg accgtcgtga tttcgccgtc ctcctgctgg agggcccgga gcaggccgag
     3001 gccgtcgagc cgcggcatca cgagatcggt gagcacgatg tccgggcgga acgcgggagc
     3061 cttcgccagc gcgtcctcgc cgtcgcacgc ggcctcgacg gcgaagcccc agccttgcag
     3121 cagctgctgg aacccgacgc gcgaagccgc atcgtcctca acgaggagga tgcggacgcg
     3181 gtcgtctggc gcggtgtcgg gcggcatgag aggtcggcgg tccccgccat tctgccccac
     3241 atagcgggct gactccaagg ggcggacgat ctacactggt gccagctccg cgcagcagcc
     3301 tggaggcgcc atgcacaaga agacggcgct cgtggtcctc gtcctcctcg ccgcgacgct
     3361 gccgacggcc tgcgcaggcc ccgacgatcg ggcgggcccg gcggcggccc gttcccagac
     3421 ggggcccagt gccagtgccg tcgagggcgg cggcggcggc gcctcgccgc tgggccgcct
     3481 ctacggcgcc acgggggccg cgcggaacct tggcgccgtg gcgtttccgc cgcgcaacga
     3541 gccgtacgcg ttccggcagg agctggaggc caagtaccgc gacggcctgg ggcggccgcc
     3601 gtcccccacc ttcgtcgatc tcgagggcga catcgtctgg acgcaggagt acgtgcggta
     3661 ccgggtgaac gggtgcgatc atccggcggc cgcgcagcgg gtgttcgatc agatcgacgg
     3721 ccgcggggtg tcgcccgtgt gcggcgacgc cgcgccgggc ccgatcgcgt ttccgccgcg
     3781 caacgagccg tacgcgttcc gcctggagct ggaggcgaag taccgcgacg ggctgcatcg
     3841 gcccgcgacc tcgtccaccg tcgacatcga gggatcgatt gtctggacgc aggagtacct
     3901 gcgataccgg gtgaaccggt gcggccacgc cgccgccgcc gccaaggtgt tcagccagat
     3961 cgacggccag gggatcgcgc caacgtgctg ggacgcgctg tcgggcgtct gggacggcga
     4021 gtccgactac ttcaacgcgc cgttcacgat ggacgtctac atgcggggcg cgagcatcct
     4081 cgggcggtac agcgaccgtc acgacacggg ctccctctgg ggcacgtacg agagcggacc
     4141 ggcggtcgtg atcaacgcgt acttcggcga cggcggcatc cgcttcgacg gggcgttcgc
     4201 cgggccggac acgatccggg gctcgatgcg cgtctcgacg ttgagccgcc tctatcactt
     4261 cacgatgacg cggcggccgt gacgccgccg ctcgcggcgc ttctcccgcg aaagccgccg
     4321 cgctagaatt cccgtcatgt cgaggctgct gagcgtcgcc gtctgggccg cggtggccgc
     4381 ggccggcgcc gccgccgtcc tcgtctcggc gctccaccgc ggcgagtcca tcaacgcact
     4441 ctggatcgtg accgccggcc tgtgctcgtt cgccatcgcc taccgcttct acagcaagtg
     4501 gctcgccgtg aaggtgctcg cgatcgacga ccggcgcgcg ccgccctcga tcaccgagcg
     4561 cgacggcaag gacttcgtgc ccacgaacac gtgggtcgtc tacggccacc acttcgccgc
     4621 catcgcggga ccggggccgc ttgtgggccc cgtgctggcc gcccagttcg gctacctgcc
     4681 cggcatgctc tggatcctgg tcggcgccac cctcggcggc ggcgtccacg acgcagtcat
     4741 cctgttcgcc tcgatgcggc gatgcggccg gtccctcggc cagatgctga aagaggaggt
     4801 caaccccgtc gtcggcgtca tcgccctcgt cagcctgctc gcgatcatga cgatcctgct
     4861 cgccgtgctg ggcctggtgg tggtcaaggc gctggcggag agcccctggg gcctcttcac
     4921 gatcgccatg acgatcccgc tcgccctggc gatggggctg gcccataccg tcgggagggt
     4981 cagcgtgcgg acggtgacgg tcttcggcgt gacggcgctc ctggccagcg tctggggcgg
     5041 cagccagctg ggcgcgtggg gcctgacgcc gtggttcgat cgcggcgccg agtggatcgc
     5101 gtgggccatc atcctgtacg gcttcgcggc gtcggtgctg ccggtgtggc tgctgctcgc
     5161 gccgcgcgac tacctgagca cgtacatgaa gctcggcacg gtggcggtgc tcgcggcggc
     5221 gatcgtgatc gtggcgccgc cgctgcacat gcccgcgctc acgcgctatg tcggcggcac
     5281 ggggcccgtg ttcgcc
//
LOCUS       DAOSIF010000179         5285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_13955_length_5285_cov_3.259273, whole genome shotgun sequence.
ACCESSION   DAOSIF010000179 DAOSIF010000000
VERSION     DAOSIF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13955_length_5285_cov_3.259273"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1957)
                     /locus_tag="PLT35_07235"
     CDS             complement(26..1957)
                     /locus_tag="PLT35_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG29057.1"
                     /translation="MTMLDRMRRHKGWLKWSLALVVVAFIILYIPDFLQKQDPAQTGA
                     LPHDQVAQVNGRGISVLDFRTAYQRQLSAYRQAYGSSISEQMLKQLGFEQQVLQQLVN
                     EQAMLAEAARLGMSVADAEVRQRILAMPQLQLNGQFVGEAQYQAILARSSPPTTPAEF
                     EARLRRDLLVEKLQALVTDWVAVTDAEADAEFTRRNEKVKVQLVHVAAAAYLGQASAT
                     DAEVAAYYEAHKQDYEVGERRKVRYVLVDTEALRTGIVVPSRELERYYNDNLDLFSTP
                     EQVRASHILFKIDGKTEADVRASAEKVLAEARAGKDFAALAKQYSEDEATAALGGDLD
                     YFGRGRMTPAFEEAAFGAEQPGLLPDLVRSPFGFHIIKVVDKKAGTTRSFDEVRPQIL
                     DQLTGERAQRQAERKAEDLAKAIVQPGDMDKAAAGRGLKVEETGFFTANEPILAFGAS
                     QQVSNSLFALADGAVSTALPVGRGFIVAVVSGKLAASIPPLDEVKDRVRNDVLRQKAG
                     ALAADKMRSAEAAIKAAADFAAAAKKAGFEAKTSELVARGAALPDVGVNAAVERAAFS
                     LPAGATSDTIAGPDGATIVKVLERKEVAPAEVAASRESVRQELIDARRSEFFSAYMTK
                     ARQRMKIEYNRGGSGQIIG"
     gene            complement(2039..2743)
                     /gene="araD"
                     /locus_tag="PLT35_07240"
     CDS             complement(2039..2743)
                     /gene="araD"
                     /locus_tag="PLT35_07240"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HOG29058.1"
                     /translation="MTARRLREAVCEANRDLFRRGLVFESFGNASGVDRARGLVVIKP
                     SGVPCKRLAPSDLVVTDLDGRPVEGRLRPSSDLPTHLEIYRAFPSVGGVAHAHSTFAT
                     AWAQAGREIPCLGTTHADYFPGPVPITRQMTPAEIRTDYEANTGRVIVRRLARLDPAA
                     TSAVLVHGHGPFAWGASAADAVRAAAVLEELARLAWATATLNPGAGPIRRALHEKHFG
                     RKHGPGAYYGQPPRER"
     gene            complement(2740..4320)
                     /locus_tag="PLT35_07245"
     CDS             complement(2740..4320)
                     /locus_tag="PLT35_07245"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOG29059.1"
                     /translation="MVVAGVDFGTLSVRVSIVDEGRGRLGSGTAEYPLTRSRHDPDLA
                     TERHADHMDALVLAMRRALADAGVPGREIRAVAIDTTGSSIVPVGEGLEPIGDYYLWC
                     DHRAWREAREITAKARELKLPALDWCGGAYSAEWGFSKLLHWLRHNPGARSRFVTVLE
                     HCDMAAAALCGITDPDQVPRSVCAMGHKWMWNPAWGGLPDEAFLQAVDPLLAGVRDRI
                     TGRFAASDAIAGRLAPAWASRLGLRAGIPIPVGALDAHWDAIGAGIRLGDVVNVIGTS
                     TCIMAISDARTLVPGVCGVVPGSVHPGYTGVEAGLSATGDIFEAIARRAGRDVAGLSE
                     AASGRRAGETGLLRLTWDNGDRTVLVNPELGGVTLGWNLTHAAEDELFAAIEGTAFHT
                     RVILERMEQHGVPVRRIINAGGIPRKNALLNRVYASVLGKPVLVPRQESTSLGSAIFA
                     FMAAGAFPTIEAAQDALCPAYDAVEPDAADARVYEDLFGLYRALYFGFGSPDAAPVAI
                     GAVLPALRETASRARSRS"
     gene            complement(4444..>5285)
                     /locus_tag="PLT35_07250"
     CDS             complement(4444..>5285)
                     /locus_tag="PLT35_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29060.1"
                     /translation="YSEKLLEASGISVEPVDLSEILGRVDRLDDDDPAVRRKLADIRA
                     YVPVGDTPPEALTKMAKLGAVLDAWMAETGVTVTALQCWTALETYFGVVPCTLMSMMS
                     HGLASSACEVDVCGAVAMHVLRLASGAPSALLDWNNNYGGDPDKAVCFHCSNLPKHFL
                     ADAQMDYQAIIAGSVGRERTYGTCVGRVKSGPMTFARFSTDDRAGRIRGYLGEGAFTD
                     DPLSTFGGAGVVRIPRLETLLRHICEHGFEHHVAASLSSVAAALHEAVTKYLGWEIYW
                     HRE"
BASE COUNT          708 a         1911 c         1876 g          790 t
ORIGIN      
        1 gcccgctggc gccaaccccc gcgccctagc cgatgatctg accgctgcct ccccggttgt
       61 actcgatctt catgcgctgc cgggccttcg tcatgtaggc ggagaagaac tcggagcgcc
      121 gggcatcgat gagctcctgg cggaccgact cgcgcgacgc ggcgacctcc gccggcgcga
      181 cctccttacg ttcgaggacc ttgacgatcg tggcgccgtc gggccccgcg atcgtgtcgc
      241 ttgtggcgcc ggccggcagg ctgaaggccg cgcgctcgac ggcggcgttc acgcccacgt
      301 ccggcagcgc cgctccgcgg gccaccagtt cgctggtttt cgcctcgaac ccggctttct
      361 tcgcggccgc ggcgaaatcg gcggctgcct tgatggcggc ctccgcggac cgcattttgt
      421 ccgccgccag cgcgccggcc ttctggcgca gcacgtcgtt gcggaccctg tctttcacct
      481 cgtccagcgg gggtatcgac gcggccagct tgccggacac gaccgcaacg atgaagcccc
      541 ggccgacggg cagcgcggtg ctgaccgcgc catccgcgag ggcgaagagc gagttcgaga
      601 cctgctggga cgcgccgaac gccaggatcg gctcgttcgc cgtgaagaag ccggtttcct
      661 cgaccttcag gccccggccg gcggcggcct tgtccatgtc gccgggctgg acgatggcct
      721 tcgccaggtc ctcggccttc cgctccgcct ggcgctgggc gcgctcgccc gtgagctggt
      781 ccaggatctg cggcctcacc tcgtcgaagc tgcgcgtcgt gccggccttc ttgtccacga
      841 ccttgatgat gtggaacccg aacggcgacc tgaccagatc gggcagcagg cccggctgtt
      901 cggcgccgaa cgccgcctcc tcgaaggccg gcgtcatccg gccgcggccg aagtagtcca
      961 ggtcgccgcc gagcgccgcc gtcgcctcat cttcggagta ctgcttcgcc agcgcggcga
     1021 aatccttgcc ggccctggct tccgccagca ccttctcggc tgaggcgcgc acgtccgcct
     1081 cggtcttgcc gtcgatcttg aagaggatgt ggctcgcgcg cacctgttcg ggcgtcgaga
     1141 acagatcgag gttgtcgttg tagtaccgct ccagctcccg cgagggcacc acgatgcccg
     1201 tgcgcagggc ctcggtgtcc acgaggacgt agcggacctt ccggcgctcg cccacctcgt
     1261 agtcctgctt gtgggcctcg tagtaggccg ccacctcggc atcggtggcg ctggcctgcc
     1321 cgaggtacgc cgctgcggcg acgtgcacga gctggacttt cactttctcg ttgcgccggg
     1381 tgaactcggc gtcggcttcc gcatcggtca cggccaccca gtcggtcacc agcgcctgga
     1441 gcttctccac gagaaggtcg cggcgcagtc tcgcctcgaa ctcggccggc gtcgtcggcg
     1501 gcgacgaacg cgccaggatc gcctggtact gcgcctcgcc cacgaactgc ccgttcagct
     1561 gcagctgcgg catcgccagg atccgctgcc gcacctcggc gtcggcgacg ctcatgccca
     1621 gccgcgcggc ctcggcaagc atggcctgct cgttgacgag ctgctgcagc acctgctgct
     1681 cgaagcccag ctgcttcagc atctgttcgc tgatgctcga tccgtacgcc tgccggtagg
     1741 ccgagagctg ccgctggtac gccgtccgga agtcgaggac gctgatgccc cggccgttca
     1801 cctgcgccac ctggtcgtgc ggcagggcgc cggtctgggc cggatcctgt ttctgcagga
     1861 agtccgggat atagaggatg atgaacgcga cgacgaccag ggcgaggctc cacttgagcc
     1921 agcccttgtg ccgccgcatg cggtcgagca tcgtcatggt gcctgtgtct ccatccagac
     1981 cagcatgcta gtctagcgtc tcgcgcgagg ggaccgcaac cgcggccgct ccgggcggtc
     2041 agcgttcccg cggcggctgc ccgtagtagg ccccggggcc gtgcttccgc ccgaagtgct
     2101 tctcgtgcag cgccctgcga atcgggccgg cgccggggtt gagcgtcgcg gtggcccacg
     2161 ccaggcgcgc cagttcctcg aggacggctg ccgcgcgaac ggcgtcggcg gccgacgcgc
     2221 cccacgcgaa cggtccgtgc ccgtgcacga gcacggcgga ggtcgcggcg gggtccagcc
     2281 gcgcgaggcg gcggacgatg acccggcccg tgttggcctc gtaatcggtg cggatctcgg
     2341 ccggcgtcat ctgccgcgtg atggggacgg ggccggggaa gtagtcggcg tgggtggtcc
     2401 cgaggcaggg aatctcgcgg ccggcctgcg cccaggcggt ggcgaaggtc gagtgcgcgt
     2461 gcgcgacgcc gccaaccgag gggaaggccc ggtagatctc caggtgcgtc ggcaggtcgg
     2521 acgaggggcg gagtcgcccc tcgacggggc ggccgtcgag atcggtcacc accaggtccg
     2581 acggcgcgag ccgcttgcag ggcacgccgc tgggcttgat gacgacgagg ccgcgcgcgc
     2641 gatcgacgcc gctcgcgttt ccgaacgact cgaagacgag gccgcggcgg aacaggtcgc
     2701 ggtttgcctc gcacaccgct tcgcgcaggc gccgcgccgt cacgaccggc tcctcgcgcg
     2761 cgacgccgtc tcgcgcagcg cgggcaggac ggcgccgatc gccaccggcg ccgcgtcggg
     2821 gctgccgaac ccgaagtaga gcgcgcggta gaggccgaag aggtcctcgt acacgcgcgc
     2881 gtcggcggcg tccggctcca cggcgtcgta ggccgggcag agcgcgtcct gcgccgcctc
     2941 gatcgtcgga aacgcccccg cggccatgaa ggcgaaaatc gccgagccga ggctcgtcga
     3001 ttcctgcctc ggcacgagga cgggcttgcc gaggacgctg gcgtagacgc ggttcaacag
     3061 cgcgttcttc cgcgggatgc cgccggcgtt gatgatccgc cggacgggca cgccgtgctg
     3121 ctccatgcgc tccaggatca cccgcgtgtg gaacgccgtg ccctcgatgg cggcgaacag
     3181 ctcgtcctcg gccgcatgcg tgaggttcca gcccaacgtg acgccgccga gctcgggatt
     3241 tacgagcacc gtccggtcgc cgttgtccca cgtcaggcgc agcagtccgg tttcgcccgc
     3301 gcggcgcccg gaggcggcct cggagagccc ggccacgtcg cggcccgcgc ggcgggcgat
     3361 cgcctcgaag atgtcgccgg tggccgagag gcccgcctcg acgccggtgt agcccgggtg
     3421 caccgacccc ggcacgacgc cgcacacccc ggggacgagg gtccgcgcgt cgctgatggc
     3481 catgatgcag gtcgaggtgc cgatcacgtt gacaacgtcg ccgagccgga tgccggcgcc
     3541 aatggcgtcc cagtgggcat cgagggcgcc gaccggaatc ggaatgccgg cccgcaggcc
     3601 caggcggctg gcccacgcgg gcgccaggcg tcccgcgatg gcgtcggagg ccgcaaaccg
     3661 ccccgtgatg cggtcgcgca cgccggccag gagcggatcg acggcctgca ggaacgcctc
     3721 gtcgggcagg ccgccccagg ccgggttcca catccatttg tgccccatgg cgcagacgct
     3781 gcgcggcacc tggtccgggt cggtgatgcc gcacagcgcg gcggccgcca tgtcgcagtg
     3841 ctcgagcacc gtcacgaagc gggaacgcgc gccggggttg tggcgcagcc agtgcaacag
     3901 cttcgagaag ccccattccg ccgagtaggc gcccccgcac cagtcgaggg ccggcagctt
     3961 cagctcgcgg gccttcgccg tgatctcgcg cgcctcgcgc cacgcgcggt gatcgcacca
     4021 caagtagtag tcgccgatgg gctccagccc ctcgccgacg gggacgatgc tcgaccccgt
     4081 ggtgtcgatg gcgacggccc ggatctcgcg gccgggtacg ccggcgtccg ccagcgcgcg
     4141 ccgcatcgcg aggaccaggg cgtccatgtg atcggcgtgt cgctcggtgg cgaggtcggg
     4201 gtcgtggcgg ctccgcgtga gcgggtactc cgcggtgccg ctgccgaggc gtccccgccc
     4261 ctcgtccacg atcgacaccc gcacgctcaa ggtgccgaaa tccacgcctg ccaccaccat
     4321 ggccatcgcc tcccgccacg catcgtgagt gcggaggccg ggccttcggg tccggcgcgg
     4381 ctgggctgac ggcccatttg ccaccggcag cgccgccgga tcccggctcc cgaacgcgtg
     4441 cccctactcc cggtgccagt agatctccca cccgaggtac ttcgtcaccg cttcgtgaag
     4501 cgccgcggcc accgacgaca ggctcgcggc gacgtgatgc tcgaagccgt gctcgcagat
     4561 gtgccgcagg agcgtctcga ggcgcgggat acggacgacg ccggccccgc cgaacgtgga
     4621 gaggggatcg tcggtgaacg cgccctcgcc gaggtagccg cggatgcgcc cggcgcgatc
     4681 atcggtggag aagcgggcga aggtcatggg cccgctcttc acgcgcccga cgcacgtgcc
     4741 gtacgtgcgc tcgcggccga cgctgccggc gatgatcgcc tggtagtcca tctgcgcgtc
     4801 cgccaggaag tgcttcggca ggttgctgca gtggaagcag accgccttgt ccgggtcgcc
     4861 gccgtagttg ttgttccagt cgaggagcgc gctcggcgcg cccgacgcga gccggaggac
     4921 gtgcatcgcc acggcgccgc acacgtccac ctcgcacgcg ctggaggcga ggccgtggct
     4981 catcatgctc atcagcgtgc agggcacgac gccgaagtag gtctcgagcg ccgtccagca
     5041 ctgcaaggca gtcaccgtga cgccggtctc cgccatccag gcgtcgagga ccgcgccgag
     5101 cttcgccatc ttcgtcaggg cctccggcgg ggtgtcgccg acggggacgt aggcccggat
     5161 gtcggccagc ttgcgccgga cggccgggtc gtcgtcgtcg agccggtcga cccgtccgag
     5221 gatctcgctg aggtccacgg gttcgaccga gatgccgctc gcctcgagca gcttctcgct
     5281 gtagc
//
LOCUS       DAOSIF010000180         5262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14026_length_5262_cov_5.331669, whole genome shotgun sequence.
ACCESSION   DAOSIF010000180 DAOSIF010000000
VERSION     DAOSIF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14026_length_5262_cov_5.331669"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLT35_07255"
     CDS             <1..366
                     /locus_tag="PLT35_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOG29061.1"
                     /translation="CIILGRAEAFASKNGPWRSVLDAVGMGLGFTFGLTFLGSLREVL
                     GSGTLFGVTVLPSWEPWVVMILPAGGFLTLGLILGGVNLITRTVEARRRRAVVEASRV
                     TARIAVTGIAPGSPARGEA"
     gene            366..938
                     /gene="rsxA"
                     /locus_tag="PLT35_07260"
     CDS             366..938
                     /gene="rsxA"
                     /locus_tag="PLT35_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOG29062.1"
                     /translation="MDLLLIFIGAAVVNNFVLSYFLGICPFIGVSGRLSSAVSMGMAT
                     TFVLVMTAMVTWVLQAYVLGPLRLEYLQTVAFILVVASLVQFVEMVIRKTSPPLYRAL
                     GIFLPLITTNCAILGLALFIQLRGYGFVQSVVFGLGAGVGFTLALCLMAGIREELDLA
                     DVPGPLRGAGITLLVAGTMALAFMGFAGMI"
     gene            958..1569
                     /locus_tag="PLT35_07265"
     CDS             958..1569
                     /locus_tag="PLT35_07265"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOG29063.1"
                     /translation="MTTRREFLNRTLMGGAAFGAGMLAGLGPFGRAAGASRWSLAARV
                     PAGAEADVEALATELGLDLGRGDWQPLAGGESPDLVLLRGTRLLDPAEWPSQAIALRA
                     RWSGHPASRRLRLTDKERRAARRAVVRSRDGILAALDLDRESSRVFRGRDRQRMALVT
                     RGGGVEVVEASCRHQHCLRQGRVGLAGERIVCAPAGLVVTLEA"
     gene            1574..2542
                     /locus_tag="PLT35_07270"
     CDS             1574..2542
                     /locus_tag="PLT35_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOG29064.1"
                     /translation="MRVPLPAGRTLLAVAVLAMAGFGLGRAIRQEADPAADLQVRTRL
                     LMGTVVEIKVPASDGRIDERAVQAALDEMSRVERVFSPSKPGGGPLTPEERSEVEAVI
                     EAGRRLAAETDGAFDLRIRDWIDLWGFESDPRVPGETEMQRVAAARRRRDPEGPLTEF
                     TFGAVAKGYAVDRAVAVLESRGVTRALVNAGGEVGAIGDGWTVGVRHPRDPGALLERV
                     RLAEGRAIATSGDYENFFVADGRRYHHLLVPATGLPATGARSVSVMAPRCAEADAWAT
                     ALFVLGPERALAAAARHPELDVMIVDADGRVTRSARWNAGVPEGRP"
     gene            2543..3400
                     /locus_tag="PLT35_07275"
     CDS             2543..3400
                     /locus_tag="PLT35_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOG29065.1"
                     /translation="MEPTLIPALVTMGGLGALFAGGLAFADRKLRVEEDPRIGDVLSQ
                     LPGANCGGCGLAGCQDFAVKVVGGEKAVTGCPVGGPDVAAEIARILGVEAGESVRLVA
                     RVLCRGTAGEHAAVKAGYVGPSSCAAKALVAGGEKACLHGCLGGGDCVDACQFDAIVL
                     NENGLPEVIDELCTGCGACVTACPRDIIELHPEDRQLFVFCRNRDDPRTARKICAVAC
                     IGCGICAKKSDGAITMVENLAVIDYTRLDPSIIPVDRCSTKAIGFLRPAAPVADESAG
                     QAASASAAG"
     gene            3437..3910
                     /locus_tag="PLT35_07280"
     CDS             3437..3910
                     /locus_tag="PLT35_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOG29066.1"
                     /translation="MTGEGREVQVEEGTVLEVLPGPPTLARVLLAAGPGCEECGARLI
                     CRTDDPGRRSLLALAPSPPPKPGERVRVCVTGARVLSAGLWAYGLPLVGLCGGLGLGW
                     LAFAGWPGRELLASAAGLACAAAPFALLWWRGRSRPPEEWFEARILAESGQDLPR"
     gene            4120..>5262
                     /locus_tag="PLT35_07285"
     CDS             4120..>5262
                     /locus_tag="PLT35_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29067.1"
                     /translation="MPHTLVVVMALVLFVLALSWVVPSGEYERVKVQTSAGERSVTVS
                     GTYRQVEKVYLGPEMVLVAPIRGFLDGALLIGFLLVIGGSFGVLQETGAVEFGIRRMT
                     ASFSRHPRLETMMIPALMLVFSLAGSVFGMAEETIPFVLIFIPLARGLGYDSIVGAAI
                     PFLGAASGFAAAVLNPFTVGIAQSIADVPLYSGMGYRLVTWAIGTAVIIAFVMRYAAR
                     VKANPEISPVRDIDLARESHQPGGADAGAGWNARHAVTLSLFASSMALLVIGVLAWGW
                     FIEEIAALFLGIGLVVGLAGGMGPSRIAKTFVTGARDMVGVVFIIACARALLVIAQDA
                     RIMDSILYGASRALSLLPRGAIAQLMFLIQCVINFFIHSGTAQAALT"
BASE COUNT          634 a         1788 c         2001 g          839 t
ORIGIN      
        1 tgcatcatcc tcggccgcgc cgaggcgttc gcgtcgaaga acggcccgtg gcgctcggtg
       61 ctggatgccg tcgggatggg gctcggcttc accttcggcc tcaccttcct cggctccctg
      121 cgggaggtcc tgggcagcgg cacgctcttc ggcgtgacgg tcctgccgtc gtgggagccc
      181 tgggtcgtga tgatcctgcc ggccggcggg ttcctgacgc tcgggctgat tctgggcggc
      241 gtcaacctga tcacccgcac cgtcgaggcc cgccgccgcc gggcggtggt cgaggcctcg
      301 cgcgtcacgg cgcgcatcgc cgtcacgggc atcgcgcccg gatcgccggc gcggggggaa
      361 gcgtgatgga tctgctgctg atcttcatcg gcgccgccgt cgtgaacaat ttcgtcctga
      421 gctacttcct cggcatctgc ccgttcatcg gcgtctcggg gcgcctctcg agcgccgtct
      481 cgatgggcat ggccacgacg ttcgtgctcg tcatgaccgc gatggtgacc tgggtgctgc
      541 aggcgtacgt gctcgggccg ctgcgcctcg agtacctgca gacggtggcc ttcatcctcg
      601 tcgtggcctc gctggtccag ttcgtggaga tggtgatcag gaagacgagc ccgccgctct
      661 accgggcgct cggcatcttc cttccgctca tcacgaccaa ctgcgcgatt ctcggcctgg
      721 cgctgttcat ccagctgcgc ggctacgggt tcgtgcagag cgtggtcttc ggcctcggcg
      781 ccggcgtcgg cttcacgctg gcgctgtgcc tgatggcggg gatccgggag gagcttgacc
      841 tggccgacgt gcccgggccg cttcgcggcg cgggcatcac gctgctggtg gcgggcacga
      901 tggccctggc gttcatgggc ttcgccggga tgatttgagg cgccgaagga gcaagcgatg
      961 acgacgcgac gggaattcct caatcggacg ctgatgggcg gcgccgcctt cggcgcgggc
     1021 atgctggccg gcctgggccc gttcgggcgc gccgcgggcg cgtcccgctg gagcctggcc
     1081 gcgcgggtgc cggccggagc agaggcggac gtggaggccc tggcgaccga actcggcctg
     1141 gacctcggcc gcggcgactg gcagccgctc gccggggggg agagccctga cttggtcctg
     1201 ctgcgcggga cgcgcctgct cgatcccgcc gagtggccgt cacaggcgat cgccctccgg
     1261 gcgcgatggt cgggtcaccc tgcgagccgg cggctgcgtc ttacagacaa ggagcgccgg
     1321 gcggcccgac gggcagtggt gcggagccgc gacggcatcc tggcggcgct ggacctggac
     1381 cgggaatcga gccgcgtttt caggggccgg gaccgacagc ggatggcgct cgtgacccgg
     1441 ggcggcggcg tcgaggtcgt cgaagcctcg tgccgccatc agcattgcct caggcagggc
     1501 cgggtcggcc ttgcgggcga gcgtatcgtg tgcgcgcccg ccggcctcgt cgtgacgctg
     1561 gaggcctgag gtcgtgagag tgccgctgcc cgcgggcagg acgctgctgg ccgttgccgt
     1621 gctggccatg gccggcttcg gcctcgggcg ggccatccgc caggaggccg atccggccgc
     1681 cgacctgcag gtccggacgc gcctgctcat gggcaccgtc gtcgagatca aggtgccggc
     1741 ctccgatggc cggatcgacg agcgggcggt gcaggccgca ctcgacgaga tgtcgagggt
     1801 cgagcgggtg ttctcgccct cgaagcccgg tggcgggccg ctgactcccg aggagcggtc
     1861 cgaggtcgag gccgtgatcg aggcgggccg ccggctggcg gccgagaccg acggggcgtt
     1921 cgacctgcgg attcgggact ggatcgatct gtgggggttc gagtccgacc ccagggtgcc
     1981 cggcgagacg gagatgcagc gggtggccgc cgcgcgccgc cgaagggatc cggagggccc
     2041 gttgaccgag ttcaccttcg gagccgtggc caagggctat gcggtggatc gcgccgtcgc
     2101 cgtgctcgag tcccggggcg tcacgcgggc gctggtgaat gcgggcggtg aggtcggggc
     2161 gatcggggac ggatggacgg tgggcgtccg gcacccccgc gatccgggcg cgctgctgga
     2221 acgcgtccgc ctcgccgaag gacgggccat cgccacctcc ggcgattacg agaacttttt
     2281 cgtggcggac ggccggcggt accaccacct gctggttccc gcgaccggcc tgccggcgac
     2341 gggggcgcgg agcgtgtcgg tgatggctcc gcgctgcgcg gaggccgacg cgtgggcgac
     2401 ggcgctgttc gtcctgggcc cggagcgggc gctggcggcg gcggcgcgcc atccggaact
     2461 cgatgtgatg atcgtggacg ccgacgggcg cgtcacgcgg agcgcccggt ggaacgcagg
     2521 cgttcccgag gggaggcctt gaatggaacc gacgttgatt ccggccctcg tcaccatggg
     2581 cgggctgggc gcgctgttcg ccggcggcct ggcgttcgcc gaccggaagc tgcgcgtgga
     2641 ggaggacccg cgcatcgggg acgtcctgtc gcagctgccc ggggcgaatt gcgggggatg
     2701 cggcctggcg ggctgccagg atttcgcggt caaggtcgtg ggcggcgaga aggcggtgac
     2761 cgggtgcccc gtcggcgggc cggacgtcgc ggcggagatc gcgcggatcc tgggcgttga
     2821 agcgggcgag agcgtccgcc tcgtggcgcg cgtgctctgt cgcggcaccg ccggcgagca
     2881 cgccgcggtg aaggccggct acgtcgggcc gtcttcgtgc gccgccaagg cgctcgttgc
     2941 cggaggggag aaggcgtgcc tgcacggctg cctcggcggc ggggactgcg tggacgcctg
     3001 ccagttcgac gcgatcgtcc tcaacgagaa cgggctgccc gaggtcatcg acgagctgtg
     3061 caccggctgc ggcgcgtgcg tgacggcgtg cccgcgagac atcatcgagc tccacccgga
     3121 agaccgccag ctcttcgtgt tctgccgcaa ccgcgacgat ccccggaccg cgcgcaagat
     3181 ctgcgccgtg gcgtgcatcg gctgcggcat ctgcgccaag aagtcggacg gcgcgattac
     3241 gatggtcgag aacctcgccg tgatcgacta cacgcggctg gatccttcca tcatccccgt
     3301 ggatcggtgc agcacgaagg cgatcgggtt cctgcgtccg gccgcgccgg tggccgacga
     3361 gtcggccggg caggcggcct cggcgtccgc ggcggggtag aggcgagcgt cgaggaagcg
     3421 cgttcaggga cgaacgatga caggcgaggg gcgtgaggtc caggtcgagg aagggacggt
     3481 cctcgaggta ttgcccgggc cgccaaccct ggccagggtg ctgctggcgg ccggccccgg
     3541 ctgcgaggag tgcggcgcgc gcctgatctg ccggaccgac gaccccgggc ggcgcagcct
     3601 gctggcgctc gccccgagcc cgccgccgaa gcccggcgag cgcgtgcgcg tctgcgtgac
     3661 cggcgcgcgg gtgctctcgg ccggcctgtg ggcctacggg ctcccgcttg tcggcctgtg
     3721 cggcggcctc ggcctgggct ggctggcctt cgccggctgg cccggccgcg agcttctcgc
     3781 cagcgccgcg ggccttgcgt gcgcggccgc gccgttcgcg ctcctctggt ggagaggacg
     3841 ctcgcgcccg ccggaggagt ggttcgaggc gcgcatcctc gccgagtccg gccaggacct
     3901 cccgcgctga accgccgcct gccggccagc gggaacatcc tcccaccccg aagctactga
     3961 gcgccgcgct aggcgctcga gcgggcgccg gactacactg tgcgccgcgg cgccgagccc
     4021 gacaggccca agagccgtct cacgccgcca gtcgtccgac accgccacgc gaggactccc
     4081 gtgccgaccg aacagaagcc ctccccgaag cgattccaga tgccgcacac cctcgtggtg
     4141 gtgatggcgc tggtcctctt cgtgctcgcg ctctcgtggg tcgtcccctc cggcgagtac
     4201 gagcgcgtga aggtccagac cagcgcgggc gagcgcagcg tcaccgtctc ggggacctac
     4261 agacaggtgg agaaggtcta tctgggcccg gaaatggtcc tcgtggcgcc gatcaggggc
     4321 ttcctcgacg gggcgctcct gattgggttc ctgctggtga tcggcggcag tttcggcgtt
     4381 ctgcaggaga ccggcgcggt ggaattcggg atcaggcgga tgacggcgtc gttctcgagg
     4441 cacccgcgcc tcgagacgat gatgattccc gcgctcatgc tcgtcttctc gctcgccggg
     4501 tcggtgttcg gcatggccga ggagacgatt cccttcgtgc tgatcttcat cccgctggcg
     4561 cggggcctgg gctacgactc gattgtcggc gcggccatcc cgttcctggg ggcggcctcg
     4621 ggcttcgcgg cggccgttct gaacccgttc accgtcggca tcgcgcagag catcgccgac
     4681 gtgcctctgt attcgggcat gggctaccgc ctggtgacgt gggcgatcgg cacggcggtc
     4741 atcatcgcgt tcgtgatgcg ctacgccgcc agggtcaagg ccaatcccga aatcagcccc
     4801 gtccgcgaca tcgatctcgc acgggagtcg caccagcccg gcggcgccga cgccggcgcc
     4861 ggctggaacg cgcgccacgc ggtcacgctc tcgctgttcg cgtcctccat ggcgctcctc
     4921 gtgattggcg tgctggcctg ggggtggttc atcgaggaga tcgcggcgct cttcctgggc
     4981 atcggcctcg tcgtcgggct cgccggaggc atggggccca gccgcatcgc gaagacgttc
     5041 gtgacgggcg ccagggacat ggtgggcgtt gtcttcatca tcgcctgcgc ccgcgcgctc
     5101 ctcgtcatcg cgcaggacgc gcggatcatg gactcgatcc tctacggcgc gtcccgggcg
     5161 ctctcgctcc tgccgcgcgg cgcgatcgcg cagctgatgt tcctcatcca gtgcgtgatc
     5221 aacttcttca tccactccgg aacggcacag gccgccctga cg
//
LOCUS       DAOSIF010000181         5238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14110_length_5238_cov_3.124831, whole genome shotgun sequence.
ACCESSION   DAOSIF010000181 DAOSIF010000000
VERSION     DAOSIF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14110_length_5238_cov_3.124831"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /locus_tag="PLT35_07290"
     CDS             <1..1868
                     /locus_tag="PLT35_07290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOG29068.1"
                     /translation="NLGMSPFIGTLLGRVGPVFSNLQSSNTSPELQAINREVAPLLTA
                     LRDDIRLNPALFARVKALWEAREGLGLTPVQSRLLDEQYKSFVRGGANLAPARKERFR
                     AINASLSELGVQFGDNLLHDTNAYRLVIEREADLAGLPASVVAAGAEAAKAAGMPGRW
                     VYTLHAPSIWPFLQYADSRELRRQILTAYITRNDHGDDYDNKANVARQAALRAERAAL
                     LGYRTHADYVLAENMAKTPAQVYGLLTQLWGPARAMALREAADQEALIRASGGAFALE
                     PWDWRYYQEQIRRKRFDLDEQALRPYFELERVRDGAFHVATRLYGLTFTPRPDLPVYH
                     PEVKAFEVKQADGSHLGVFYTDYHPRPGKRVGAWSSSFRSTRVKDGRRITPVVVNVCN
                     FSRPAGDEPALLTLEETSTLFHEFGHALHSLLSQTPYRGLGGFPRDFVEVPSQIMENW
                     ALEPEVLAVYARHYRTGEAIPTALVDKIRQSAQFDQGFVTVEYLAAAFLDMDWHTLAS
                     AEPVDAAAFERASLERIQLPPVIVSRYRSPYFSHIFGAGGGYSSGYYSYIWSEVLDQD
                     AFQAFREKGLFDPATARGFRAILEQGGTKDPMDLYKAFRGREPSVEPLLRKRGLK"
     gene            complement(1882..3741)
                     /gene="aspA"
                     /locus_tag="PLT35_07295"
     CDS             complement(1882..3741)
                     /gene="aspA"
                     /locus_tag="PLT35_07295"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOG29069.1"
                     /translation="MKDLIDQLRASDLFAGLPEAALASIAGVGARRAVPAGGVLFRQG
                     DPRVTCYLLTSGRVEAVQDTGDGPERVIVLGPGDTLGEGALLHAARHSVTARALTDVE
                     AAAFERDAVRALMERDGSLAIELLGRVSLLQHRRALYSPIARRNREQAYGSTRSRTET
                     DLLGTRQVPADALFGIQSLRALENFPITGIEVGHFPAFVRSLAMVKQAAARANARLGL
                     LDPGVAAAIDRASQEIIDGRWHGHFLVDMVQGGAGTSTNMNANEVIANRALEILGRRR
                     GEYAACHPNDHVNMSQSTNDVYPTAVRLATVFMLASLLQALARLRDALRAKGAEFKDV
                     IKMGRTQLQDAVPMTLGQEFEAFAVTTGEDIERLEETARLLHEVNLGGTAIGTGICAD
                     PRYAGAAVEELAAIAGLPLVLATNLVEATPDTGAFVMFSGTLKRAAVKLSKLCNDLRL
                     LSSGPRCGLAEINLPPRQPGSSIMPGKVNPVIPEVVNQVAFHVIGNDLTITLAAEAGQ
                     LQLNVMEPVLAFNLFQSLQMLTSAVNTLTAHCITGITANAEHCRALVAGSIGIVTATL
                     PALGYKRATEVAREAITTGVPVREIIRAKGWLTDAELDEILSPEAMTRPRPLS"
     gene            complement(3797..4459)
                     /locus_tag="PLT35_07300"
     CDS             complement(3797..4459)
                     /locus_tag="PLT35_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOG29070.1"
                     /translation="MLLIDELLAEHRLIERVSEAFLAYAAGPAGDARAGDTARFLRFF
                     RGYANRFHHAREEDTLFAALRERGGLPADRGPLAVLVADHRRMEALLGEIEALPAGAA
                     GAPHPAGFRTLAASYVHALWHHIDAEDSVLFPECEPRLRRNGVAELPSRPMDADERAA
                     KAEGESLVLKYPPAGPRDGDPMRGDGCIMCPAYGVTCRGLELEWWNEWEWDEFEDRMG
                     GD"
     gene            4848..>5238
                     /locus_tag="PLT35_07305"
     CDS             4848..>5238
                     /locus_tag="PLT35_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG29071.1"
                     /translation="MAGTDSVVGRYRWVVCGLIFLATTVNYIDRQILALIKEFLDAEL
                     GWTNEQFGLVNSAFQGAYAAGLFLFGWFVDKYGTKIGYAVSITFWSVAALAHAAVASV
                     GGFFAARVFLGVSEGGNFPAAIKGIALW"
BASE COUNT          718 a         1837 c         1915 g          768 t
ORIGIN      
        1 ccaacctggg catgagcccg ttcatcggca ccctgctcgg gcgggtgggc ccggtgttct
       61 cgaacctgca gtcgtcgaac acgtcgccgg agctgcaggc catcaaccgc gaggtggcgc
      121 cgctcctgac ggcgctgcgg gacgacatcc ggctgaaccc ggcgctcttc gcgcgcgtga
      181 aggcgctctg ggaggcgcgg gagggcctgg ggctgacgcc cgttcagtcg aggctgctcg
      241 acgagcagta caaatcgttc gtgaggggcg gcgccaacct cgcgccggcc cggaaggaac
      301 ggttccgggc gatcaacgcg tcgctctccg agctgggcgt ccagttcggg gacaacctgc
      361 tgcacgacac gaacgcgtac cggctcgtga tcgagcgcga ggccgatctc gccgggctgc
      421 cggcgtcggt cgtggccgcc ggcgcggagg ccgcgaaggc ggcgggaatg ccgggacggt
      481 gggtctacac gctccacgcg ccgagcatct ggccgttcct gcagtacgcg gacagccgcg
      541 agctgcgccg ccagatcctg acggcctaca tcacgcggaa cgatcacggc gacgactacg
      601 acaacaaggc gaacgtcgcg cgccaggcgg cgctgcgggc cgagcgcgcc gcgctcctcg
      661 gctaccggac gcacgccgac tacgtgctcg ccgagaacat ggcgaagacg ccggcccagg
      721 tgtacggcct gctcacgcag ctctgggggc cggcgcgcgc catggccctg agggaggcgg
      781 ccgaccagga agcgctcatc agggcctccg gcggcgcctt cgcgctggag ccctgggact
      841 ggcgctacta ccaggagcag atcaggcgca agcggttcga cctggacgag caggcgctgc
      901 ggccgtactt cgagctcgag cgcgtgcgcg acggcgcgtt tcacgtcgcc acccggctgt
      961 acggcctgac gttcacgccg cggcccgatc tccccgtgta ccatcccgaa gtgaaggcct
     1021 tcgaggtgaa gcaggccgac ggctcgcacc tcggcgtgtt ctacacggac taccatcccc
     1081 ggcccggcaa gcgcgtcggg gcgtggagca gctcgttccg gagcacgcgc gtcaaggatg
     1141 ggcggcgcat caccccggtg gtcgtgaacg tgtgcaactt ctcccggccc gcgggcgacg
     1201 agcccgcgct gctcaccctc gaggagacca gcaccctgtt ccacgagttc ggccacgcgc
     1261 tgcactcgct cctgtcgcag acgccctacc gcggcttggg cggcttcccc agggacttcg
     1321 tcgaggtgcc gtcgcagatc atggagaact gggccctcga gcccgaggtg ctcgccgtgt
     1381 acgccaggca ctaccgcacg ggcgaggcca tcccgacggc cctcgtggac aagatcaggc
     1441 agtcggcgca gttcgatcag ggcttcgtca cggtcgagta cctggcggcg gcgttcctcg
     1501 acatggactg gcacacgctg gcgtcggccg agccggtgga cgccgccgcg ttcgagcgcg
     1561 cgtcgctcga gcgcatccag ctgccgccgg tgatcgtgtc gcgctaccgg agcccgtact
     1621 tcagccacat cttcggggcc gggggcgggt actcgtcggg gtactactcc tacatctgga
     1681 gcgaggtgct ggaccaggac gcgttccagg cgttccggga gaaggggctg ttcgacccgg
     1741 cgacggcacg cgggttccgc gcgatcctgg agcagggcgg cacgaaggat ccgatggatc
     1801 tctacaaggc gttccggggc cgcgagccga gcgtcgagcc gctgctccgc aaacgcggcc
     1861 tgaaatagcg ccggccgccg cctacgacaa cgggcggggc cgggtcatgg cctccgggct
     1921 gaggatctcg tccagctcgg cgtccgtgag ccagcccttc gcgcggatga tctcccggac
     1981 cggcacgccg gtcgtgatgg cctcgcgcgc cacctcggtc gcgcgcttgt agccgagggc
     2041 cggcagcgtc gcggtgacga tgccgatgct ccccgcgacg agcgcgcggc agtgctccgc
     2101 gttcgccgtg atgccggtga tgcagtgggc tgtcagggtg ttcaccgcgg acgtcagcat
     2161 ctgcaggctc tggaacaggt tgaacgcgag caccggctcc atgacgttga gctggagctg
     2221 tcccgcctcg gccgccagcg tgatggtgag atcgttgccg atcacatgga acgccacctg
     2281 gttcacgact tcggggatca ccgggttcac cttcccgggc atgatgctgc tccccggctg
     2341 gcgcggcggc aggttgatct cggccaggcc gcaccgcggc ccgctcgaga gcaggcgcag
     2401 gtcgttgcag agcttgctga gcttgacggc ggcccgcttg agcgtgccgg agaacatgac
     2461 gaaggccccc gtgtccggcg tcgcctcgac gagattggtc gccagcacca gcggcagtcc
     2521 ggcgatcgcg gcgagctcct cgacggccgc cccggcgtag cgcggatcgg cgcagatgcc
     2581 cgtgccgatg gcggtgccgc ccaggttcac ctcgtgcagc agccgcgcgg tttcctcgag
     2641 ccgctcgatg tcctcgcccg tggtgaccgc aaacgcctcg aactcctgcc cgagcgtcat
     2701 gggcacggcg tcctggagct gcgtgcggcc catcttgatg acgtccttga actcggctcc
     2761 cttcgcgcgc agcgcgtcgc gcaggcgcgc caaggcctgc agcagcgacg cgagcatgaa
     2821 gacggtggcg aggcggaccg ccgtcgggta gacgtcgttg gtgctctggc tcatgttcac
     2881 gtgatcgttc gggtggcacg ccgcgtactc gccgcgccgg cgcccgagga tctcgagggc
     2941 acggttggcg atgacctcgt tggcgttcat gttcgtggac gtgcctgcgc cgccctgcac
     3001 catgtccacc aggaagtgcc cgtgccaccg gccgtcgatg atctcctggc tggcccggtc
     3061 gatggccgcc gcgacgcccg ggtcgagcag gcccaggcgc gcgttggccc gggccgccgc
     3121 ctgcttcacc atcgccaggc ttctcacgaa ggccgggaag tggccgactt cgatgccggt
     3181 gatggggaag ttctcgaggg cgcgcaggct ctggatgccg aacagggcgt cggcgggcac
     3241 ctgccgggtg cccagcaggt cggtctcggt ccggctgcgg gtgctgccgt aggcctgctc
     3301 gcggttccgg cgcgcgatgg gcgagtagag ggcccgccgg tgctgcagca gcgagacgcg
     3361 gccgagcagc tcgatggcga ggctgccgtc gcgctccatc agcgcccgga cggcgtcgcg
     3421 ctcgaaggcg gcggcctcga cgtccgtcag cgcgcgggcc gtgacgctgt ggcgcgcggc
     3481 gtggagcagc gcgccttcgc cgagcgtgtc gcccgggccg aggacgatga cgcgctcggg
     3541 gccgtcgccc gtgtcctgga cggcctcgac gcggcccgac gtcagcaggt agcacgttac
     3601 ccgcgggtcg ccctgcctga agagcacccc gcccgcgggc acggcgcggc gggcgcccac
     3661 cccggcgatg gaggcaagcg cggcttcggg caggccggcg aacaggtccg acgcgcgcag
     3721 ctgatcgatg aggtctttca tggcaagccg tgctccagcg ggggacgcac tccgcgatcg
     3781 gcgcgcgggc ggcggcctag tcgccgccca tccgatcctc gaattcgtcc cattcccatt
     3841 cgttccacca ttcgagctcg agcccgcggc aggtgacgcc gtaagcgggg cacatgatgc
     3901 agccgtcgcc gcgcatgggg tctccgtcac ggggcccggc cggcgggtac ttcagcacga
     3961 gagactcgcc ctcggccttc gccgcgcgct cgtccgcgtc catggggcgg gagggcagtt
     4021 cggcgacccc gttgcggcgg agcctcggct cgcactcggg gaagaggacg gagtcctcgg
     4081 cgtcgatgtg gtgccacagc gcgtggacgt aggatgcggc gagcgtgcgg aacccggcag
     4141 gatgcggcgc tccggccgcg ccggcgggca gcgcctcgat ctcgccgagc agcgcctcca
     4201 tgcgccggtg atccgcgacc aggaccgcca gggggccccg atcggcgggg agcccgcccc
     4261 gctcgcggag cgccgcaaag agggtgtcct cctcgcgcgc gtgatggaag cggttcgcat
     4321 agccgcggaa gaagcgcagg aacctcgccg tgtcgcccgc ccgggcgtcg cccgccgggc
     4381 cggcggcata cgcgaggaac gcctccgaga cgcgttcgat gagacggtgt tcggccagaa
     4441 gctcgtcgat gagcagcatc cggcgccatt gtacctgagg ccgcgcgaag agccgaagcc
     4501 gacgggccgg cccagcggga gaggcccgcg cgcctgtccg tccgccggcg gctgccgtgc
     4561 ggcgcgatcc gctgcgcgag gttgcggcgc gggcggtccc cgcgttgccg gcgcggtccg
     4621 gcccgcggcg ggcgcgacga tccggccggc gcccgcgctt gacaggggct gccccgggtg
     4681 ctacttgtga acccgcgcgt tgccctcgtc ggtctgacct ggccgtcccc gagccgcatg
     4741 cctgacgctc acggatccgt cgccgccgag cccggggcgc cgccgcagcg gcccgcgccc
     4801 tggcccgggc gagggcagcc acacaggcac cacggaaggg gcgttccatg gcgggcaccg
     4861 acagcgtcgt cggcagatac cggtgggtcg tttgcggcct gatcttcctg gcgaccaccg
     4921 tcaattacat cgaccggcag atcctcgcgc tcatcaagga gttcctggat gccgagctcg
     4981 ggtggaccaa cgagcagttc ggcctggtga actccgcctt ccagggcgcc tacgccgccg
     5041 gcctcttcct gttcgggtgg ttcgtcgaca agtacggcac gaagatcggc tacgcggtgt
     5101 cgatcacctt ctggagcgtc gccgccctgg cgcacgcggc ggttgcctcg gtcggagggt
     5161 tcttcgccgc gcgggtcttc ctgggggtga gcgagggcgg caacttcccg gcggccatca
     5221 agggcatcgc gctgtggt
//
LOCUS       DAOSIF010000182         5236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14116_length_5236_cov_2.998649, whole genome shotgun sequence.
ACCESSION   DAOSIF010000182 DAOSIF010000000
VERSION     DAOSIF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14116_length_5236_cov_2.998649"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /gene="fusA"
                     /locus_tag="PLT35_07310"
     CDS             <1..1960
                     /gene="fusA"
                     /locus_tag="PLT35_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG29072.1"
                     /translation="VMDWMAQEQERGITITSAATTCFWRDHRINIIDTPGHVDFTAEV
                     ERSLRVLDGAVAVFDAVSGVEPQSETVWRQADKYRVPRICFVNKMDRVGADFLDTVAQ
                     IRRKLQANPVVVQLPIGAEAGFQGVVDLVRMKALRYRDETPGSGFAVEEIPADLLGQA
                     REYRERLIEKACEADDAVLEKYLHGSDIGEDEIRAALRKRTIESVRREQAPVVPVLCG
                     AAFRNLGVQPLLDAVVDYLPSPADIPPVIGLVPGKADRQAERPADDRAPFSALVFKIM
                     TDPFVGQLAYIRAYSGAMQSGGTVFNATRDKTERVGRLLKMHANKREEIREVCAGDIA
                     AAVGLRSVTTGDTICDRQHPILLESMDFPAPVISLAIEPKTKADQEKLGQGLAKLQAE
                     DPTFRAHTDAETGQVVIDGMGELHLEILVDRLRREFGVDASVGNPQVAYKETLTRAAE
                     GEGRYIRQTGGRGQYGHVRLRLEPREAGAGFAFASAIVGGAIPREFLKPIEEGIREAM
                     TAGVLASYPVDDVGVTGHDGSFHEVDSSEMAFKIAGSMAFKDAAKKAAPILLEPVMRV
                     EVVVPEEYMGDIIGDLTGRRGRITSMEARGGTQIVHSRVPLSEMFGYATDLRSKTQGR
                     ATHTMHFDRYEPAPQAVSDEIVARVMGR"
     gene            2016..3215
                     /gene="tuf"
                     /locus_tag="PLT35_07315"
     CDS             2016..3215
                     /gene="tuf"
                     /locus_tag="PLT35_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOG29073.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHIDHGKTTLTAAMTKVAASKGWAKY
                     VSYDEVAKASESQGRRDETKILTIATSHVEYGTAKRHYAHVDCPGHADYIKNMITGAA
                     QMDGAIVVVAATDGPMPQTREHVLLARQVEVPYLVVALNKVDAVDDPELLDLVELEVR
                     DLLKSYGFPGDDLPVVRVSALKALEGDAEAEAGILKLMEALDEYIPLPERQVDKPFLM
                     PIEDVFSISGRGTVVTGRVERGKVKVGEEVEIVGFRPTLKKVVTGVEMFRKLLDEGVA
                     GDNIGVLLRGTEKDDVERGQVLAKPGSITPHTKFTARVYVLTKEEGGRHTPFFNGYRP
                     QFYLRTTDVTGAMTLGAGVEMIMPGDNTDVTAELITPVAIEKGSKFAIREGGRTVGAG
                     TVTEILA"
     gene            3308..3457
                     /gene="rpmG"
                     /locus_tag="PLT35_07320"
     CDS             3308..3457
                     /gene="rpmG"
                     /locus_tag="PLT35_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOG29074.1"
                     /translation="MRDIITLACTACKRRNYNTTKNKKKTTERLEMKKYCPFCRTHTP
                     HRETK"
     gene            3462..3537
                     /locus_tag="PLT35_07325"
     tRNA            3462..3537
                     /locus_tag="PLT35_07325"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3495..3497,aa:Trp,seq:cca)
     gene            3592..3846
                     /gene="secE"
                     /locus_tag="PLT35_07330"
     CDS             3592..3846
                     /gene="secE"
                     /locus_tag="PLT35_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG29075.1"
                     /translation="MVDNVKEAPARALDGVTGWYGNLRTFLGEVRNELRRVTWPSRKE
                     VYGTTVVVILTSIFFGLYLWGLDQVFEKSVLSLFRYFGAK"
     gene            3855..4385
                     /gene="nusG"
                     /locus_tag="PLT35_07335"
     CDS             3855..4385
                     /gene="nusG"
                     /locus_tag="PLT35_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOG29076.1"
                     /translation="MTKQWYIVQTYSGFERKVAESLRQRIQAYGLQDEIGEVLIPTED
                     TVEMRGGRKVVTPKRFFPGYILVEMAMTDGAWHVVKNTPKVTGFVGAGAKPTPLTKEE
                     VDQILLQVSAAAEKPKPKYSFDKGDQVRINEGPFTSFNGVVDEVNVDRNTLKVMVTIF
                     GRSTPVELDFLQVEKI"
     gene            4534..4959
                     /gene="rplK"
                     /locus_tag="PLT35_07340"
     CDS             4534..4959
                     /gene="rplK"
                     /locus_tag="PLT35_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG29077.1"
                     /translation="MAKKVQAVVKLQIPAGKATPAPPVGTALGPHGVNIMGFCKEFNA
                     KTASQEGLIIPVLVTIYADRTFSFVTKTPPAAVLLKRAANIAKGSAEPNKTKVGTVTA
                     RQVEEIARAKMPDLNADTVEAAIRTVVGAARSMGLDVIE"
     gene            5038..>5236
                     /locus_tag="PLT35_07345"
     CDS             5038..>5236
                     /locus_tag="PLT35_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG29078.1"
                     /translation="MRNVGKKFAAARGQVPDRPLPLEEAVPLVQRLKFAAFDETVEMA
                     FRLGVDPKHADQMVRGTVVLPH"
BASE COUNT          924 a         1579 c         2038 g          695 t
ORIGIN      
        1 cgtcatggac tggatggcgc aggagcagga gcggggcatc acgatcacgt cggcggccac
       61 gacgtgcttc tggcgcgacc accggatcaa catcatcgac acgcccgggc acgtggactt
      121 cacggccgag gtggagcggt cgctgcgggt gctggacggc gccgtcgcgg tcttcgacgc
      181 ggtgagcggc gtggagcccc agtcggagac ggtgtggcgc caggcggaca agtaccgcgt
      241 gccccgcatc tgcttcgtca acaagatgga ccgggtgggc gccgacttcc tcgacacggt
      301 cgcgcagatc cgccgcaagc tccaggcgaa cccggtggtc gtccagctgc cgattggcgc
      361 cgaggcgggc ttccagggcg tcgtcgacct ggtgcggatg aaggccctcc gctaccggga
      421 cgagacgccg ggatcgggtt tcgccgtcga ggaaattccc gcggacctgc tcggccaggc
      481 ccgcgagtac cgcgagcggc tcatcgagaa ggcctgcgag gccgacgacg cggtcctgga
      541 gaagtacctg cacggcagcg acatcggcga ggacgagatc agggcggcgc tccggaagcg
      601 gaccatcgag tcggtccgcc gcgagcaggc gcccgtcgtc cccgtgctct gcggggcggc
      661 gttcaggaac ctgggcgtgc agcccctgct cgacgcggtg gtggactacc tgccgtcgcc
      721 cgccgacatc ccgcccgtca tcgggctggt gcccggcaag gccgatcggc aggccgagcg
      781 gccggccgac gaccgcgcgc ccttctcggc gctcgtgttc aagatcatga ccgacccgtt
      841 cgtcggccag ctcgcctaca tccgcgccta ctcgggggcc atgcagtcgg gcgggacggt
      901 gttcaacgcg acgagggaca agaccgagcg cgtcgggcgc ctcctgaaga tgcacgcgaa
      961 caagcgcgag gagatcaggg aggtctgcgc gggcgacatc gccgcggccg tcggcctccg
     1021 gagcgtgacg accggcgaca cgatctgcga ccggcagcac cccatcctcc tcgagtcgat
     1081 ggacttcccg gcgccggtca tctccctggc catcgagccg aagacgaagg cggaccagga
     1141 gaagctcggg caggggctcg cgaagctgca ggccgaggac ccgacgttcc gcgcgcacac
     1201 cgacgcggag acgggccagg tggtcatcga cgggatgggc gagctgcacc tcgagatcct
     1261 cgtggaccgc ctgaggcgcg agttcggcgt ggacgccagc gtggggaacc cgcaggtggc
     1321 ctacaaggag acgctgacgc gggcggccga gggcgagggg cgctacatcc gccagaccgg
     1381 cggccgcggg cagtacggcc acgtgaggct ccggctggag ccgcgcgagg ccggcgcggg
     1441 gttcgccttc gccagcgcca tcgtgggcgg cgccatcccg cgcgagttcc tcaagcccat
     1501 cgaggagggc atccgcgagg cgatgaccgc cggcgtgctg gcgagctacc ccgtggacga
     1561 cgtcggcgtg acggggcacg acgggtcgtt ccacgaggtg gactcctcgg aaatggcctt
     1621 caagatcgcc ggctcgatgg cgttcaagga cgccgcgaag aaggccgccc cgatcctgct
     1681 cgagccggtg atgcgcgtcg aggtggtggt gcccgaggag tacatgggcg acatcatcgg
     1741 cgacctcacc ggccggcgcg ggcggatcac gtcgatggag gcccgcggcg ggacgcagat
     1801 cgtccactcc cgcgtgcccc tctcggagat gttcggctac gccacggacc tgcggtcgaa
     1861 gacccagggc cgcgcgacgc acacgatgca cttcgaccgg tacgaacccg cgccccaggc
     1921 cgtgagcgac gagatcgtcg cgcgcgtcat ggggcgctga caggacggcg caggcggctg
     1981 cgccgcggca ctgggcgcac gaacggagac gagccatggc gaaggaaaag tttgatcggt
     2041 cgaaaccgca cgtgaacgtg gggacgattg ggcacatcga ccacgggaag acgacgctga
     2101 cggcggcgat gacgaaggtg gcggcgtcga aggggtgggc gaagtatgtg tcgtatgacg
     2161 aggtggcgaa ggcgtcggag tcgcaggggc gtcgggacga gacgaagatc ctgacgattg
     2221 cgacgtcgca cgtggagtat gggacggcga agcggcacta cgcgcacgtg gactgcccgg
     2281 ggcacgcgga ctacatcaag aacatgatca cgggtgcggc gcagatggac ggggcgattg
     2341 tggtggtggc ggcgacggac gggccgatgc cgcagacgcg ggagcacgtg ctgctggcgc
     2401 ggcaggtgga ggtgccctac ctggtggtgg cgctgaacaa ggtggacgcg gtcgacgacc
     2461 cggagctgct ggacctggtg gagctggagg tgcgggacct gctgaagtcg tatgggttcc
     2521 cgggggacga cctgccggtg gtgcgggtgt cggcgctgaa ggcgctggaa ggggacgcgg
     2581 aggcggaggc ggggattctg aagctgatgg aggcgctgga cgagtatatt ccgctgccgg
     2641 agcggcaggt ggacaagccg tttctgatgc cgatcgagga tgtgttttcg atttcggggc
     2701 gggggacggt ggtgacgggg cgggtggagc gggggaaggt gaaggtcggg gaggaggtcg
     2761 agattgtggg cttccggccg acgctgaaga aggtggtgac gggcgtcgag atgttccgga
     2821 agctgctgga cgagggggtg gcgggggaca acatcggcgt gctgctgcgg gggacggaga
     2881 aagacgacgt ggagcggggg caggtgctgg cgaagccggg gtcgatcacg ccgcacacga
     2941 agttcacggc gcgggtgtat gtgctgacga aggaagaggg cgggcggcac acgccgttct
     3001 tcaacgggta tcggccgcag ttttacctgc ggacgacgga cgtgacgggg gcgatgacgc
     3061 tgggggcggg cgtggagatg atcatgcccg gggacaacac ggacgtgacg gcggagctga
     3121 tcacgccggt ggcgatcgag aaggggtcga agttcgcgat ccgcgagggc gggcggacgg
     3181 tgggcgcggg gacggtgacg gagattctgg cctaggggcc aggggcgggg cgcggggcgc
     3241 tagtcggccg ggggcgggac ggcctcgccc cgagcgccta gccccgagcg cctgaaggta
     3301 gacagcaatg cgcgacatca tcacgctggc gtgcaccgcg tgcaagcgcc gcaactacaa
     3361 cacgaccaag aacaagaaga agaccaccga gcgcctggag atgaagaagt actgcccctt
     3421 ctgccggacg cacacgccgc accgggagac gaagtgagca caggccaata gctcaactgg
     3481 tagagcaccg gtctccaaaa ccgggggttg ggggttcgag tccctcttgg cctgccagat
     3541 tcagccgccc ccgggccgcg tggatcgggg cggcagaggc gagagcaccg catggtcgac
     3601 aacgtgaagg aggcgcccgc cagggcactc gacggcgtga cggggtggta cggcaacctg
     3661 cgcaccttcc tgggcgaagt gcgcaacgag ctgaggcggg tcacctggcc gtcgcgcaag
     3721 gaagtgtacg ggacgaccgt ggtcgtcatc ctgacgtcga tcttcttcgg cttgtatctc
     3781 tggggcctgg accaggtctt cgagaagagc gtcctgtccc tgttcaggta cttcggggcc
     3841 aaatgaccga cgtcatgacg aagcagtggt acatcgtgca gacgtactcg gggttcgagc
     3901 gcaaggtcgc cgagtcgctg cggcagcgga tccaggccta cggcctgcag gacgagatcg
     3961 gcgaggtgct gatcccgacc gaggacaccg tcgagatgcg cggcggccgc aaggtggtca
     4021 cgccgaagcg cttcttcccg ggctacatcc tcgtcgagat ggcgatgacc gacggcgcgt
     4081 ggcacgtcgt gaagaacacg ccgaaggtca ccgggttcgt cggcgccggc gccaagccca
     4141 ccccgctcac gaaggaggag gtcgatcaga tcctcctgca ggtgtccgcc gcggccgaga
     4201 agcccaagcc caagtactcg ttcgacaagg gcgaccaggt gcggatcaac gaggggccgt
     4261 tcacgagctt caacggcgtc gtcgacgagg tgaacgtcga tcggaacacg ctgaaggtca
     4321 tggtgaccat cttcgggcgg tccacgccgg tcgagctgga cttcctgcag gtggagaaaa
     4381 tctaggggcc gggcgccgga gagcccgggg cgcgcggctc gggattcggg cccggaggcg
     4441 ggaacgcggg acggacatcg gggcgctcgg cggggaaggg ccgccgggcg gcggaagcgg
     4501 acaacaggcg actgatcatc gaaagcaccg aacatggcga agaaggtaca ggcggtcgtg
     4561 aagctgcaga tcccggcggg aaaggcgacg ccggcgcccc cggtgggcac ggccctgggg
     4621 ccccacggcg tcaacatcat ggggttctgc aaggagttca acgcgaagac cgcgagccag
     4681 gagggcctga tcatcccggt gctcgtgacg atctacgccg accggacgtt cagcttcgtg
     4741 acgaagacgc cgccggcggc cgtgctgctc aagcgcgcgg ccaacatcgc caaggggtcg
     4801 gccgagccga acaagacgaa agtgggcacg gtgaccgcca ggcaggtcga ggagattgcc
     4861 agggcgaaga tgccggacct gaacgccgac acggtggagg cggcgattcg gaccgtcgtc
     4921 ggtgcggcgc ggtccatggg gctggatgtc atcgagtaga cccgggcgcc gccgcgccgg
     4981 cggcgccgcc ggtgggaggg cccggaggcc gggcccggtt ggaccacgga ggtatccatg
     5041 cgcaacgtag gcaagaagtt cgccgccgcg cgggggcagg tgcccgacag gcccctcccc
     5101 ctggaggagg cggtccccct cgtccagcgg ctcaagttcg ccgccttcga cgagaccgtc
     5161 gagatggcgt tccggctggg cgtggacccg aagcacgccg accagatggt gcgcggcacg
     5221 gtggtgctgc cgcacg
//
LOCUS       DAOSIF010000183         5225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14150_length_5225_cov_4.000000, whole genome shotgun sequence.
ACCESSION   DAOSIF010000183 DAOSIF010000000
VERSION     DAOSIF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14150_length_5225_cov_4.000000"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PLT35_07350"
     CDS             <1..1256
                     /locus_tag="PLT35_07350"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29079.1"
                     /translation="SIKQGPVSIELAFAGGKVTAEDVSALLGLVGRDLLFEVMAAVAD
                     ENGAAAFELAGRAVESGYDLRMLCRELSRLVRDLLVLSVDPSRFGDPEVAPETDRSRL
                     EALLPRFSREDLLRAFDVLARAEDEVRRAAEPRTAFEMALLRWIHLRRLTPLADLIDR
                     LERGGGPPAGSGGRSSESSLPFGGQVRPAAPSASASSAPGARRQAAAPLRQAPPARQG
                     GPAAAVSRPPAAPTGRASDAGRWPANDPDEGLDAGLDSRPAPRSPAAPPSGGPALKDA
                     FVAGIQKGRPMIFQTVVTEAQRIDVEGSRIVFRYASNQTMLADQVAQQRQWLESLASE
                     VAGRRMSVAAEVAASGPAGPARRNAPAAAAASPAAPKPDERGLEPEAGAPRDLRAEAL
                     RDPAIQSLLDVIPAELRDVTELKDP"
     gene            1253..1549
                     /locus_tag="PLT35_07355"
     CDS             1253..1549
                     /locus_tag="PLT35_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOG29080.1"
                     /translation="MNIQAMMKQAQRMQEQMQRQMADLRIEASSGGGMVKVVVNGLKQ
                     IQSLTIDPEVVSRDDVEMLQDLILAAVNDATRRADEAMSQQLGGMMGGLKLPGM"
     gene            1565..2161
                     /gene="recR"
                     /locus_tag="PLT35_07360"
     CDS             1565..2161
                     /gene="recR"
                     /locus_tag="PLT35_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOG29081.1"
                     /translation="MFQPEPLTRLIEQLQRLPGIGRKSAQRLAFHVLKTPRADADLLC
                     EAIRDVKERVTYCSVCHNVTDADPCPVCTDDSRDTHLICVVEEPPNVLAIERSRDYNG
                     TYHVLMGALSPLQGIGPDDLRIKSLLARIGAGGVEEVILATNPNVEGEATAIYLARLL
                     KPMGVRVTRIAMGVPVGSDLEYADDVTMHKAMEGRREV"
     gene            2163..2774
                     /locus_tag="PLT35_07365"
     CDS             2163..2774
                     /locus_tag="PLT35_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HOG29082.1"
                     /translation="MLPPSFQVPASIVLLAGGALACFAGYRLFRQVLAVYGFILGALI
                     ATSVVGQAGTWVTALAALGGGLAGALVLVAGYFVGVALVGAALGASLVHLGWRLVGGD
                     PHWAAVLACAALGAIAAMAFQRHVIIAATAFGGAWTMLVGAAALMLGRGATVGSSATD
                     VWVVYPGYEGPASGWVYVAWVAVALTGVYVQLHAGGRTRPRKK"
     gene            complement(2914..5160)
                     /locus_tag="PLT35_07370"
     CDS             complement(2914..5160)
                     /locus_tag="PLT35_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG29083.1"
                     /translation="MRHHSRPPHLLPRRAPAAAVAVLTLGALALPAGQAERPSAQRTP
                     VFRSGRDLVVVNVVVRDKSGAPVRGLTRDDFTVFEDNRPQTIESFDLEELDAVTGRFE
                     IADSLLAAAAKPAPGAARAPAGEAAKAAEVPAPLPSRPVADLRDRRLMVLFFDLSAME
                     PDQLARAVQSGRDYVTARLSPADLIGVVTLGASLEVAQDFTSDRALLLAALDQISPVE
                     GPGLAATAAADAESAADTGNAFTADDTEFGIFSTDRRLDALRSLADVLAGVEQKKSVI
                     YFSGGMTRQGMDNQAAMRSVIDRAVRANMSIYAADTRGLQALPAGGEASQASVRGSGA
                     FSGQSMRGAFDSLSSSQDSLATLAEDTGGRAFFDANEFGEVYDQVVKDTTAYYLLGYT
                     STNPASDGRYRRIRVSLKRPGYKLEHRSGYYAPRDFSHSGRDDREQQMQEHLLSDLSP
                     TDLPVHGLAGYFRLKPNRYFVPMSFIVPGSQVQFSRASDRERATLDVLGVIRDPQQRI
                     VGWIRDTVRLTVAAAEDVRRKNVQYDTSFELPPGRYAVKLVIRENQLGTVGSFETPLV
                     VPDIERQGLKVSSVVLASQRHPAPAKKGVTNPLLRDGQLLTANVARVVTAAQPLYFFF
                     EVYDPAKPGADAPGAVPDARPGPPPATGQPAGPRAPRTGAGAAAPPDAARVLASVACY
                     RGSQRALQTPLVTFDRLNAADRGAVAVQIEIGPGQLPPGPYTCQVNIVDDAAGTFAFP
                     RVPLYVRR"
BASE COUNT          674 a         1878 c         1922 g          751 t
ORIGIN      
        1 ggtcgatcaa gcagggcccc gtctcgatcg agctcgcctt cgccggcggg aaggtcacgg
       61 ccgaggacgt gtcggcgctc ctcggcctgg tcggcaggga cctcctcttc gaggtgatgg
      121 ccgcggtcgc cgacgagaac ggcgccgccg ccttcgagct tgcgggccgg gcggtcgagt
      181 cgggctacga cctgcggatg ctttgccgcg agctgtcgcg gctcgtgcgc gatctgctgg
      241 tgctgtcggt cgatccgtcg cggttcggcg acccggaggt ggcccccgag accgaccgca
      301 gccgcctcga ggcgctgctg ccccgcttct cgcgcgaaga cctgcttcgc gcgttcgacg
      361 tgctggcgcg cgccgaagac gaggtcaggc gcgccgcgga gccgcgcacc gcgttcgaga
      421 tggcccttct gcggtggatc cacctgcgca ggctgacgcc gctcgccgat ttgatcgatc
      481 gcctcgagcg cggcggcggg ccgcctgccg gttcgggcgg ccgttcttcg gaaagctccc
      541 tcccgttcgg cgggcaggtg cggcctgccg ccccttccgc ctccgcttcg tccgcgccgg
      601 gggcgcgccg gcaggctgcg gctccgcttc ggcaggcgcc gccggcccgt cagggcgggc
      661 cggcggcggc cgtcagccgt cctcccgcgg cgcccacagg ccgcgcttcg gacgccggcc
      721 gatggccggc gaacgatccg gacgaaggcc tcgatgcggg cctcgattcg cggcccgcgc
      781 cgcgctctcc cgcggcgccg ccgagcggag gacctgcctt gaaggacgcc ttcgtcgccg
      841 gcatccagaa ggggcggccg atgattttcc agacggtcgt cacggaggcc cagcgtatcg
      901 acgtcgaagg ctctcgcatc gttttccgat acgcctcgaa ccagaccatg ctcgccgacc
      961 aggtggcgca gcagcggcag tggctggagt cgctggcctc tgaggtggcc ggccggcgga
     1021 tgtcggtcgc ggccgaggtg gccgcgtcgg ggcctgccgg gccggcgcgg cggaacgcgc
     1081 ccgccgccgc ggccgcgagc cccgcggcac cgaagccgga cgagcggggc ctcgagccgg
     1141 aggccggcgc gccgcgcgac ctgcgggccg aggcgctccg cgacccggcg atccagtcgc
     1201 tcctcgacgt catccccgcc gagctgcggg acgtgacgga gctgaaggac ccatgaacat
     1261 ccaggccatg atgaagcagg cgcagcggat gcaggagcag atgcagcgcc agatggccga
     1321 cctccggatc gaagcctcct ccggcggcgg catggtgaag gtcgtggtga acggcctgaa
     1381 gcagattcag tcgctcacga tcgaccccga ggtcgtgtcc agggacgacg tggagatgct
     1441 gcaggacctg attctcgccg ccgtcaacga cgcgacgcgt cgcgccgacg aagcgatgtc
     1501 gcagcagctg ggcgggatga tgggcggcct gaagctgccc ggcatgtagg cgcggcggtt
     1561 tcgcatgttc cagcccgaac ccctcacgcg gctcatcgag cagctgcagc gcctgccggg
     1621 catcggccgc aagagcgcgc agcgcctggc cttccacgtg ctgaagacgc cgcgcgcgga
     1681 cgccgacctg ctgtgcgagg ccatccgcga cgtcaaggag cgcgtgacgt actgctcggt
     1741 gtgccacaac gtcaccgacg ccgacccgtg tcccgtctgc acggacgact cgcgcgacac
     1801 gcacctcatc tgcgtcgtcg aggagccgcc gaacgtgctg gccatcgagc gcagccgcga
     1861 ctacaacggc acctaccacg tcctgatggg cgccctctcg cccctgcagg gcatcggccc
     1921 cgacgatctg cgcatcaaga gccttctcgc ccgcatcggc gcgggcggcg tggaggaggt
     1981 catcctcgcg accaatccga acgtcgaggg cgaagcgacg gccatctacc tggcgcggct
     2041 gctgaagccc atgggcgtgc gcgtgacgcg catcgcgatg ggcgtgcccg tcggcagcga
     2101 cctcgagtac gccgacgacg tcaccatgca caaggccatg gagggccgca gggaggtctg
     2161 acatgctccc gccgtcgttc caggtgcccg cgtcgatcgt cctgctcgcc ggcggggcgc
     2221 tggcctgttt tgccggctac cggctcttca ggcaggtcct cgccgtctac gggttcatcc
     2281 tcggcgcgct gatcgccacc tcggtggtgg ggcaggccgg cacgtgggtc accgccctgg
     2341 cggcgcttgg cggcgggctc gccggcgcgc tggtgctggt agccggctac ttcgtgggcg
     2401 tggcgctcgt gggcgccgcg ctcggcgcgt ccctcgtgca cctcggctgg cggcttgtcg
     2461 gaggagatcc ccactgggcg gcggtgctgg cgtgcgccgc cctcggcgcg attgccgcga
     2521 tggcgttcca gcggcacgtg atcatcgcgg cgacggcgtt cggaggcgcc tggacgatgc
     2581 tcgtgggcgc cgcggcgctg atgctcggca gaggcgcgac ggtggggtcg tcggcgaccg
     2641 acgtgtgggt cgtctacccc ggctacgagg gcccggcgag cggctgggtc tacgtggcgt
     2701 gggtggccgt cgccctcacc ggggtgtacg tccagctgca cgcgggcggc cggacgcggc
     2761 cgcggaagaa gtaggccggt tctcggcacc agcgacccgg gctctggagc tcccgggctc
     2821 catcctcact ctgcattccg aattccggct tctggattcc gacttccgga ttcggggctc
     2881 cccggccgcg ccagacttcg ccttcgcggc cggtcatctc cgcacgtaga gcggcacccg
     2941 cggaaacgcg aacgtgcccg ccgcgtcatc cacgatgttc acctggcagg tgtacgggcc
     3001 gggcggcagc tggcccgggc caatctcgat ctggacggcc acggccccgc ggtccgccgc
     3061 gttgaggcgg tcgaaggtca cgagcggcgt ctgtagcgcg cgctggctgc cgcggtagca
     3121 cgcaacgctc gccagcacgc gcgccgcatc gggcggcgcg gccgcgcccg cccccgtccg
     3181 cggcgcgcgc gggccggccg gctgtcctgt cgccggcggg ggcccaggcc tcgcatccgg
     3241 gaccgcaccc ggagcgtccg cgcccggctt cgccgggtcg tacacctcga agaagaagta
     3301 cagcggctgt gcggcggtca ccacgcgggc gacgttggcg gtcagcagct ggccgtcccg
     3361 caggagcgga ttcgtgacgc ctttcttcgc gggcgcggga tggcgctgcg aggcgagtac
     3421 gacggaactc accttcagcc cctgccgctc gatgtcgggc accacaagcg gcgtctcgaa
     3481 cgatccgacc gtgcccagct ggttctcgcg gatgacgagc ttgacggcgt agcgcccggg
     3541 cggcagctcg aagctcgtgt cgtactgcac gttcttgcgc cgcacgtcct cggcggccgc
     3601 caccgtcagc cgcaccgtgt cgcggatcca cccgacgatg cgctgctggg ggtcgcggat
     3661 gacgcccagg acgtcgagcg tcgcccgttc cctgtcgctc gcccgggaga actgcacctg
     3721 cgagcccggc acgatgaagg acatcggcac gaagtaccgg ttcggcttca gccggaagta
     3781 gcccgccagt ccgtgcaccg gcaggtccgt gggggagagg tccgagagca ggtgctcctg
     3841 catctgctgc tcgcggtcgt cgcgccccga gtgcgagaag tcgcgcggcg cgtagtagcc
     3901 cgaccggtgc tccagcttgt agcccggccg cttcagggag acccggatgc ggcggtagcg
     3961 gccgtcggac gccgggttcg tgctcgtgta gccgagcagg tagtacgccg tcgtgtcctt
     4021 cacgacctgg tcgtacacct cgccgaactc gttggcgtcg aagaacgcgc ggccgcccgt
     4081 gtcctcggcg agcgtcgcca gcgagtcctg cgagctcgac aggctgtcga acgcgccccg
     4141 catcgattgc ccggaaaacg cccccgagcc gcgcacgctg gcctggctcg cctcgccgcc
     4201 ggcgggcagc gcctggaggc cccgggtgtc ggccgcgtag atggacatgt tcgcgcggac
     4261 cgcgcggtcg atgaccgagc gcatcgccgc ctggttgtcc atgccctgcc gggtcatgcc
     4321 gccgctgaaa tagatgaccg acttcttctg ctcgacgccc gccagcacgt ccgcgagcga
     4381 ccgcagcgcg tcgaggcggc ggtccgtgct gaagatgccg aactcggtgt cgtcggcggt
     4441 gaaggcgttg cccgtgtcgg cggcgctctc cgcgtccgcc gccgccgtgg cggcgagccc
     4501 ggggccctcg acggggctga tctgatcgag cgcggcaagc agcagcgcgc ggtccgacgt
     4561 gaagtcctgg gcgacttcca gcgatgcgcc gagcgtcacg acgccgatga gatcggcggg
     4621 cgagaggcgc gccgtgacgt agtcgcggcc ggactgcacc gcccgcgcca gctggtccgg
     4681 ctccatcgcg ctcagatcga agaagagcac catgagccgg cggtcgcgca ggtcggcgac
     4741 gggcctggac ggcagcggcg ccggcacctc cgccgccttc gcggcttcgc ccgcgggcgc
     4801 acgggcggcg cccggcgcgg gcttggccgc cgccgcgagc aggctgtcgg cgatctcgaa
     4861 acggcccgtg acggcgtcga gctcctcgag gtcgaagctc tcgatcgtct gcggccggtt
     4921 gtcctcgaag acggtgaaat cgtcgcgggt cagtccgcgg acgggcgccc cgctcttgtc
     4981 gcgcacgacc acgttgacca cgaccaggtc gcggccggag cggaagacgg gcgtccgctg
     5041 cgccgaaggc cgctcggcct gcccggccgg gagcgcgagc gcccccagcg tcaggacggc
     5101 gacggccgcc gccggcgcgc ggcgcgggag gaggtgcggc gggcgcgagt ggtgcctcat
     5161 ctcagagccc cagcctgacg ttgaactgca cgctccgcat cgagcgcacc gagaggacct
     5221 gcctg
//
LOCUS       DAOSIF010000184         5211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14196_length_5211_cov_4.564973, whole genome shotgun sequence.
ACCESSION   DAOSIF010000184 DAOSIF010000000
VERSION     DAOSIF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14196_length_5211_cov_4.564973"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLT35_07375"
     CDS             complement(<1..285)
                     /locus_tag="PLT35_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29084.1"
                     /translation="MELVVRKFDLLKELQLFQGIVERKNTIPILANVLIDAKGEVMRL
                     AATDLEVGLRAECKASIARAGALTLPAKKLFEIVKELPDTRNLLIKYVSEE"
     gene            complement(572..1987)
                     /gene="dnaA"
                     /locus_tag="PLT35_07380"
     CDS             complement(572..1987)
                     /gene="dnaA"
                     /locus_tag="PLT35_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG29085.1"
                     /translation="MESHNVWDRVLARAEAKVNRHSYYTWFHPSSLLSDTGELLRVRV
                     PDPVFRDWLTKHYAGIIAEALSEVGRAGTRVEFVTDGPSGAMLTAQEAAPPIPPEEAA
                     LIEAVASEPAAGDELTAAGSSWAPPAPDATPGVGLNVRYTFESFVVGPSNQFAEAASR
                     AVAEAPARSYNPLYIYGGVGLGKTHLMHAIGHYVLRHNQHFKLTYISTERFMNEMINA
                     VKTDRVIDFRERYRGVDVLLVDDIQFLAGKEGTQNEFFHTFNALYDAQKQIVLSSDCA
                     PNEIPSLEERLRSRFAWGLIADIQPPDLETKVAILKKKADAESVPLPDNVAIYIAEKI
                     KSNIRELEGSLIRLIAFASLTGRDVSIDLAQEVLKNVIGQDDRAVTVDMIQKAVADYY
                     QLKIADMRARNNSKSVALPRQIAMYLCKALTHASLPEIGRSFGGKHHSTVIHSIRKIE
                     ALRKRDRDFNSVINTLIDRMR"
     gene            2313..2456
                     /gene="rpmH"
                     /locus_tag="PLT35_07385"
     CDS             2313..2456
                     /gene="rpmH"
                     /locus_tag="PLT35_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG29086.1"
                     /translation="MKRTFQPNVRRRKKTHGFRRRMKTQDGQRILARRRAKGRKRLTV
                     SDE"
     gene            2470..2868
                     /gene="rnpA"
                     /locus_tag="PLT35_07390"
     CDS             2470..2868
                     /gene="rnpA"
                     /locus_tag="PLT35_07390"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOG29087.1"
                     /translation="MTSGQQGILPIAVPAKRDERFRPREHIRKRAEFAAIYDRGRRVP
                     GRLMTLFLLENGLEWPRLGIAATKKFGCAAQRNRAKRLVREAFRRHKPSRGLDIVVVP
                     RREMNEAGFARVEAEYAAVVERRAGRLRGR"
     gene            2858..3145
                     /gene="yidD"
                     /locus_tag="PLT35_07395"
     CDS             2858..3145
                     /gene="yidD"
                     /locus_tag="PLT35_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG29088.1"
                     /translation="MAASVPSRAVRAALAIYKSFVSPLFAGSCRYLPSCSDYMAEAVE
                     RHGAAAGIALGVRRLARCQPLGGSGLDPVPRQRPWRALVSRRPCREPGAEP"
     gene            3155..4867
                     /gene="yidC"
                     /locus_tag="PLT35_07400"
     CDS             3155..4867
                     /gene="yidC"
                     /locus_tag="PLT35_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOG29089.1"
                     /translation="MEKRVVLAVVLSFVVLYLYQATFAPKTPRRAAPPAAEQPAGSTA
                     AESPGQPAAPPQGGAQAAGPAAAAPIVAAVADTEEHDAIIETDTLRAVFSNRGGVLKS
                     WRLKTYKDKAGRPVDLVPQTLPDDPKPFSIKTGDAGADARANAALFRTEGGAPRRDPQ
                     SGAFLVRFEYAEAGGVAVRKAFSVDPRAFTVDAVVEASIGGSPVNVSVVMGPGPGDVE
                     TSESNRFRMGARANLYREGKVERYDAAALDRQPLMEGVMRWAGVDDHYFLSAVLARTR
                     NVRVEYAPRRTQDAQGKTQHTFVGYTVNLPGQAIDATFFLGPKAFALLKSLDAELVRA
                     IDYGMFAWLVVPLLNALNWVNSYVGNYGWSIIALTFLINLALLPLRHKSVVSMKKMQA
                     LQPEIKAIQERYGKLKAADPARQKMNQELMELYKQRGVNPASGCVPMLLTMPILFAFY
                     SMLAYSIELRGAPFILWIKDLAAHDPYYVAPVLTGLSMLVQQRMTPQTGADPMQQKMF
                     MIMPVVFTVMFLWAPSGLNIYWLFNNVLAIAQQYFTNRLLGPARVPRPAAERRSAKKG
                     TRSA"
     gene            4864..>5211
                     /locus_tag="PLT35_07405"
     CDS             4864..>5211
                     /locus_tag="PLT35_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29090.1"
                     /translation="MSSDLSTRLVEFVSAVTGAMGLSLTATVTETPDLLRVNLAGDGA
                     EVLVRRRGEALGALQHIVSAVFRDDAPGGQRVVVDSLGFRQDKDAELRRMAAFLAEKA
                     AASGAPQEIGPLNP"
BASE COUNT          823 a         1783 c         1733 g          872 t
ORIGIN      
        1 ctcttcgctg acgtatttta tcaggaggtt gcgcgtgtcc ggcagttcct tgacgatctc
       61 gaacagcttc ttcgccggca gcgtgagcgc cccggcccgc gcgattgacg ctttgcactc
      121 ggcgcggagg cccacctcga gatccgtcgc cgccaggcgc atcacctcgc ccttggcgtc
      181 gatgagcacg ttggccagga tcgggatcgt gttcttgcgc tcgacgatgc cctggaacaa
      241 ctgcagctct ttcagcagat cgaatttgcg tacgaccagt tccatggtgt cctgcccccg
      301 aaggccgccc cctgtggcgc cgcgaatctc tcccgctaca ggaacgagat tgaaacaaga
      361 aattatactg taaggatcgg gtgttgggaa gcaggaaaac gccccgtaag cgattgtttg
      421 ccaaggactt gccgcggcga aaaggtgttg acttcctgcg ggaggagcgt gtgcgggcgc
      481 gcgcttccgg gaggggccgc cgcgttccca caaggcgtcc gcagcgcggc cctgcggacg
      541 agtgtgcaga cgccccgccg ggccgccttt cctaccgcat ccggtcgatg agggtgttga
      601 tgacgctgtt gaaatcgcgg tcccgcttgc gcagcgcctc gatcttccgg atcgagtgga
      661 tgaccgtcga gtggtgcttc ccgccgaagc tccggccgat ctccggcagc gacgcgtgcg
      721 tgagcgcctt gcagaggtac atcgcgatct gcctcggcag cgccaccgac ttcgagttgt
      781 tgcgcgcgcg catgtcggcg atcttgagct ggtagtagtc cgccaccgcc ttctggatca
      841 tgtccaccgt caccgcgcgg tcgtcctggc cgatgacgtt cttcagcacc tcctgggcca
      901 ggtcgatgga cacgtcgcgg cccgtgagcg acgcgaatgc gatgaggcgg atgagcgatc
      961 cctccagctc gcggatgttc gacttgatct tctccgcaat gtagatggcc acgttgtccg
     1021 gcagcggcac gctctccgcg tccgccttct tcttgagaat ggccactttc gtctcgagat
     1081 ccggcggctg gatgtccgcg atcaggcccc aggcgaaccg cgaccgcagc cgctcctcga
     1141 gcgagggaat ctcgttgggg gcgcagtcgc tgctcagcac gatctgcttc tgcgcgtcgt
     1201 acagggcgtt gaacgtgtgg aagaactcgt tctgcgtgcc ttccttcccg gcgaggaact
     1261 ggatgtcgtc gaccagcagg acgtccacgc cccggtaccg ctcgcggaag tcgatcacgc
     1321 ggtccgtctt caccgcgttg atcatctcgt tcatgaagcg ctcggtcgag atgtaggtca
     1381 gcttgaaatg ctggttgtgc cggaggacgt agtgcccgat cgcgtgcatg aggtgcgtct
     1441 tgcccagccc caccccgccg tagatgtaga gcgggttgta cgaccgcgcc ggcgcctcgg
     1501 ccactgcccg cgacgcggct tcggcaaact ggttcgacgg cccgacgacg aagctctcga
     1561 acgtgtagcg cacgttcagg ccgacgccgg gcgtggcgtc cggcgcaggc ggggcccagg
     1621 aggacccggc cgccgtcaac tcgtcccctg ccgccggctc ggacgccacc gcctcgatga
     1681 gggccgcctc ctccggcgga atcggaggcg cggcctcctg cgccgtcagc atggcgccgg
     1741 aaggcccgtc cgtcacgaac tcgacgcgcg tgccggccct gcccacctcg gacagggctt
     1801 cagcgatgat gccggcgtag tgcttcgtca gccagtcgcg gaacaccggg tcgggcacgc
     1861 gcacccgaag cagctctccc gtatcgctca gaagagaact ggggtggaac caggtgtagt
     1921 agctgtgccg gttgaccttg gcttcggccc tggcgagaac gcgatcccac acgttgtggc
     1981 tctccatggc cacccatcct cttcgttaat aaagccttac caaacaatat cttgccgact
     2041 ttcgccgttc cgagcggcgc ttcgcgcgag ccgctggcct ccgggactcg cggaccgccg
     2101 ccgtattcgg gcccgatccc ggctttcgcc cccttcgata tccacagcct tttccacatc
     2161 tgtggaaaac ccgcctcccc gagcaggctg ggttttccac aggcggagcc ttgacgataa
     2221 cacctgacgg gcaaatcgcg caggaaaagt tgacgggttc acgaaggacc gctagtatgg
     2281 gagattcggt tctctgcggt tagggtgtct gcatgaagcg cacatttcag ccgaacgtac
     2341 gccgccggaa gaagacccac gggttccgga ggcggatgaa gacccaggat ggccagcgga
     2401 ttctcgcgcg ccgccgcgcg aagggccgga agcgcctcac ggtgtccgac gaatagcccg
     2461 gaggcggccg tgacgtccgg ccagcaaggc atcctcccca tcgccgtccc ggcgaagcgc
     2521 gacgagcggt tccgtccgcg ggagcacatt cgaaagcgcg cggagttcgc agcgatctac
     2581 gatcgagggc gccgcgttcc aggccgcctg atgacgctct tcctgctgga gaacgggctg
     2641 gagtggccgc ggcttggcat tgccgccacg aagaagttcg gctgcgccgc gcagaggaac
     2701 cgggccaagc gcctggttcg agaagcattc aggcgccaca aaccctcccg cggccttgac
     2761 atcgttgtgg ttccgcgtcg ggagatgaac gaggccggct tcgcgcgtgt cgaagccgag
     2821 tacgcggccg tagtcgagcg gcgcgccggg aggctccgtg gccgctagcg tgccgtcgcg
     2881 cgccgtccgt gcagcgctgg cgatctacaa gtcgttcgtg tccccgctct tcgccgggtc
     2941 gtgccgctac ctcccctcct gttcggacta catggccgag gcggtggaac gccacggggc
     3001 ggcggcgggc attgcgttgg gcgtccgccg gctggccagg tgccagcccc tgggtggcag
     3061 cgggctggat cccgtgccgc ggcagaggcc gtggcgcgcg ctcgtgtcgc gtcggccgtg
     3121 ccgcgagcct ggcgccgaac cctgatcggg ccatatggaa aaacgcgtcg tccttgccgt
     3181 cgtcctgtcg ttcgtggtgc tctatctcta ccaggcgaca ttcgcgccga agaccccgcg
     3241 gcgcgcggcg ccgcccgccg ccgagcaacc ggcgggttca accgctgcgg aaagcccggg
     3301 acagcccgcg gcgccgccgc agggcggcgc acaggcggcc gggcccgccg ccgcggcgcc
     3361 gatcgtcgcg gcggtggcgg acaccgagga acacgacgcc atcatcgaga ccgacacgct
     3421 ccgggccgtc ttctcgaacc gcggcggcgt cctgaagagc tggaggctga agacctacaa
     3481 ggacaaggcc ggccgcccgg tcgacctggt tccccagacc cttccggacg atccgaagcc
     3541 gttctcgatc aagaccggcg acgccggcgc cgacgcgcgg gccaacgccg ccctcttccg
     3601 caccgagggc ggggccccca ggcgcgaccc gcagagcggc gcgttcctcg tgcgcttcga
     3661 gtacgccgag gcgggcggcg tggcggttcg aaaggcgttc tccgtcgatc ccagggcctt
     3721 cacggtcgat gcggtcgtcg aggcctccat cggcggctcc cccgtcaacg tctcggtcgt
     3781 catgggcccg ggcccgggcg acgtggagac gagcgagagc aaccgcttca ggatgggcgc
     3841 gcgcgcgaac ctgtaccgcg agggcaaggt cgagcgctac gacgccgcgg cgctcgacag
     3901 gcagccgctg atggaaggcg tcatgcgctg ggccggcgtc gacgaccact acttcctgtc
     3961 cgccgtgctg gcgcgcaccc ggaacgtccg cgtggagtac gcgccccggc ggacccagga
     4021 cgcccagggc aagacgcagc acacgttcgt cggctacacc gtgaacctgc cgggacaggc
     4081 catcgacgcg acgttcttcc tcggcccgaa ggcgttcgcc ctgctcaagt cgctcgatgc
     4141 cgagctcgtg cgggcgatcg actacggcat gttcgcctgg ctcgtggttc cgctcctcaa
     4201 cgccctgaac tgggtcaact cgtacgtcgg caattacggc tggtcgatca tcgccctcac
     4261 cttcctcatc aacctcgcgc tcctgccgct gcgccacaag agcgtcgtgt cgatgaagaa
     4321 gatgcaggcg ctgcagccgg agatcaaggc aatccaggag cgctacggca agctcaaggc
     4381 ggcggatccc gcccgccaga agatgaacca ggagctgatg gagctctaca agcagcgcgg
     4441 cgtcaacccg gccagcggct gcgtgcccat gctcctgacg atgccgatct tgttcgcgtt
     4501 ctactcgatg ctggcctact ccatcgagct gcgcggggcg ccgttcatcc tgtggatcaa
     4561 agacctcgcc gcgcacgacc cgtactacgt cgcgccggtc ctgaccggcc tctcgatgct
     4621 cgtccagcag cggatgacgc cgcagacggg cgcggacccg atgcagcaga agatgttcat
     4681 gatcatgccg gtggtgttca ccgtcatgtt cctctgggcg ccgagcggct tgaacatcta
     4741 ctggctcttc aacaacgtgc ttgccatcgc gcagcagtac ttcacgaacc ggctgctcgg
     4801 cccggcgcgc gtcccgcgcc cggcggccga acggcggtcc gcgaagaagg gcactcgatc
     4861 cgcatgagca gcgacctcag cacccgactc gtggaattcg tgagcgccgt gaccggggcc
     4921 atgggcctgt cgctgacggc gaccgtgacg gagacgccgg acctgctgcg ggtgaacctt
     4981 gccggcgacg gggccgaggt cctggtgcgc cggcggggcg aagcgctcgg cgcgctccag
     5041 cacatcgtct ctgccgtctt ccgcgacgac gcgccggggg gccagcgcgt ggtcgtggac
     5101 agcctcgggt tccgccagga caaggatgcg gagctgcggc gcatggcggc gttcctggcc
     5161 gagaaagccg cggcttccgg agcgccccag gaaatcggcc ccctcaaccc g
//
LOCUS       DAOSIF010000185         5203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14226_length_5203_cov_3.157537, whole genome shotgun sequence.
ACCESSION   DAOSIF010000185 DAOSIF010000000
VERSION     DAOSIF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14226_length_5203_cov_3.157537"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="ftcD"
                     /locus_tag="PLT35_07410"
     CDS             complement(<1..856)
                     /gene="ftcD"
                     /locus_tag="PLT35_07410"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOG29091.1"
                     /translation="MIKLVECVPNFSEGRDRTVIDAIAQALSSVSGVKLLDVDPGADT
                     NRTVYTLVGPPDAVAEAAFRGASRAAELIDMRAHKGAHPRVGALDVCPFVPVAGVTMA
                     ECAELARGVGRRMGDELGIPIYFYEHAAASDARRSLADVRAGEYEGLAARLADPAWAP
                     DAGPARFNARSGATVVGAREFLLAYNVNLNTRDRRLANEVALSIREAGRARRDAQGQI
                     VRDRDGAPVKAPGRLKAVRAIGWYIEHYRQAQVSINLLDFRTTPLHVVFETAREEAEK
                     LGLLVTGSE"
     gene            855..2660
                     /locus_tag="PLT35_07415"
     CDS             855..2660
                     /locus_tag="PLT35_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOG29092.1"
                     /translation="MATAYVGRGRAGKLALRLQCPVMKRLLLGFLALAVAAAPLARAQ
                     SRRAPQAPASMPPFSIVEATIPEMRAAMDARRITSRELVRQCLVRIATYEERLNAAIA
                     VSPRALEEAEALDRERAEGRLRGPLHGIPIALKDNIHTTSMPTTGGALAFEGLVPPYE
                     ATLVKHLRDAGAVIIAKTVMTELANWVATGMPGNYSALGGYGMNPYDPRRDPREASGD
                     GRPVMGTGGSSSGIGTAASFWAASVGTETSGSILSPSNATMLVGIKPTVGRISRHGVI
                     PITADQDTAGPMARTVADAAILLGALEGAAPDPNDPATKACAPPPGRDYTAFLNPRAL
                     AGARIGIPRAFFYDRTTPPGASQPRGGLSDAQAARMAEAIDILRQQGAVIVDPADIPS
                     IVTPDRDRNILLWPVCGGLDQAKGKDADCSVVFKYGMKRDFNAWLASLGDAAPVKSLG
                     GLRVWNVAHQRAGAIKYGQALLDVSDEMSLQGDRARYQADREKDLRLSGAEGIDAVMK
                     AERLDALLFPGSSGASIAAKPGYPTVIVPFGFVDAAGGGAAGGRGGQDFPEGFQPKPS
                     PFGVSFTGMACSEPRLVALAYAFEQATKRRVPPGL"
     gene            complement(2676..>3670)
                     /locus_tag="PLT35_07420"
     CDS             complement(2676..>3670)
                     /locus_tag="PLT35_07420"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR01822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOG29093.1"
                     /translation="AAVPPALQRGARFICGTQAVHKQLEEKLSEFLGTEDTILYSSCF
                     DANGGLFETLLGERDAVISDALNHASIIDGIRLCRAQRHRYANSDMQDLEAKLHASAD
                     ARFRMIATDGVFSMDGYIANLPAICDLAERHGALVMVDDSHAVGFVGARGRGTHEHHG
                     VMPRVDVLTGTLGKALGGASGGYTSGRREIIELLRQRSRPYLFSNTLCPSIAGATLKV
                     LDLLSQSTALRDALASNTRYFREGMSQAGFRVLPGTHPICPVMIGDAALAGRVADAML
                     EKGVYVIGFSFPVVPKGEARIRTQISAAHTRADLDRAIGAFAEVKREMGLDTGA"
     assembly_gap    3671..3680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3681..3879)
                     /locus_tag="PLT35_07425"
     CDS             complement(<3681..3879)
                     /locus_tag="PLT35_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29094.1"
                     /translation="MYGSYQHFASQQLDDIRAAGTFKDERVILTPQGTTIRVSDGRPV
                     LNLCANNYLGLAQHPEVREAAR"
     gene            complement(4135..4211)
                     /locus_tag="PLT35_07430"
     tRNA            complement(4135..4211)
                     /locus_tag="PLT35_07430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4175..4177),aa:Pro,seq:tgg)
     gene            complement(4303..>5203)
                     /locus_tag="PLT35_07435"
     CDS             complement(4303..>5203)
                     /locus_tag="PLT35_07435"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOG29095.1"
                     /translation="GQWGSALACACRLMGLECMVYMVRVSYDTKPYRRIMIQSYGGQI
                     VPSPSPLTQAGRAILAEHPDSPGSLGIAISEAVEDAAGRDDTKYSLGSVLNHVILHQT
                     IIGQEAKKQMELAGEYPDILIGCHGGGSNFAGLTFPFLEDKLKGRRPALRAIAVEPAS
                     CPTLTKGPREYDFGDTAGLTPLLMMHTLGHNFVPPPVHSGGLRYHGSAPLVSHLLAAG
                     MIEAEAYDQRSVFESAVQFARCEGTIPAPESAHAVHAAMRQALLARESGEAKVILFNL
                     SGHGNFDMAAYDAFLSGQMDGGV"
BASE COUNT          727 a         1846 c         1877 g          743 t
ORIGIN      
        1 gctcggaccc ggtcacgagc aggccgagct tctccgcctc ctccctggcc gtctcgaaga
       61 cgacgtggag gggggtcgtg cggaagtcga ggaggttgat cgacacctgc gcctggcggt
      121 agtgctcgat gtaccagccg atggcgcgca cggccttcag ccggcccggc gccttcaccg
      181 gcgcgccgtc gcggtcgcgg acgatctggc cctgcgcgtc gcgcctggcg cggccggctt
      241 cgcggatgct gagggcgact tcgttggcga gccggcgatc gcgcgtgttg aggttcacgt
      301 tgtacgcgag caggaactcg cgggcgccga cgacggtggc gccgctgcgg gcgttgaagc
      361 gggccggccc ggcgtcgggc gcccaggcag ggtcggccag cctggcggcg agcccttcgt
      421 actcgcccgc gcggacgtcg gcgaggctgc ggcgcgcgtc ggaggcggcc gcgtgctcgt
      481 agaaatagat cgggattccc agctcgtcgc ccatgcgccg gccgacgccg cgggccagct
      541 ccgcgcattc ggccatcgtc acgcctgcga cgggcacgaa cgggcacacg tcgagggcgc
      601 ccacgcgcgg gtgcgcgccc ttgtgcgcgc gcatgtcgat gagctcggcg gccctgctgg
      661 cgccgcggaa cgcggcctcc gccacggcgt cgggggggcc gacgagggtg tagaccgtgc
      721 ggttggtgtc cgcgccggga tcgacgtcga gcagcttcac gccgctcacg ctcgagagcg
      781 cctgcgcgat ggcgtcgatg accgtccgat cgcggccctc ggagaagttg ggaacgcatt
      841 ccacgagctt gatcatggcc acagcgtacg tcggccgcgg ccgcgcgggc aagctcgccc
      901 ttcggctaca atgcccggtc atgaagcgac tcctgctcgg tttcttggcg ctcgcagtcg
      961 cggccgcgcc cctggcccgc gcgcagtcca ggcgggctcc ccaggcgccc gcctccatgc
     1021 cgccgttcag catcgtcgag gccacgatcc cggagatgcg cgccgcgatg gacgcgcgcc
     1081 ggatcacctc gcgtgaactg gtccggcagt gtctggtgcg gatagccacg tacgaggagc
     1141 ggctcaatgc cgcgatcgcc gtcagcccgc gcgctctcga ggaggcggag gcgctcgacc
     1201 gcgagcgcgc cgagggcagg ctccgcggcc cgctgcacgg gatcccgatc gccctgaagg
     1261 acaacatcca cacgacctcc atgccgacga ccggcggcgc gctggctttc gagggcctcg
     1321 tgccgcccta cgaggccacg ctcgtgaagc acctccgcga cgcgggggcc gtgatcatcg
     1381 ccaagaccgt gatgaccgag ttggcgaact gggtcgccac cggcatgccc gggaactact
     1441 ccgcgctggg cggctacggc atgaacccgt atgatccgcg gcgcgacccg cgcgaggcga
     1501 gcggggacgg gcggccggtc atgggaaccg gaggatcgag ttcgggcatc ggcacggccg
     1561 cgagcttctg ggcggccagc gtcgggaccg agacatcggg ctccatcctg agcccgtcga
     1621 acgcgacgat gctcgtcggc atcaagccga cggtcggccg catcagccgc cacggcgtga
     1681 tcccgattac cgccgatcag gacaccgccg gcccgatggc ccggaccgtc gcggacgcgg
     1741 cgatcctgct cggcgcgctc gaaggcgcgg cccccgaccc gaacgacccc gcaacgaagg
     1801 cctgcgcgcc gcccccggga cgcgactaca ccgcgttcct gaacccccgg gctctcgcgg
     1861 gcgcgcgcat cggcatcccc cgcgcgttct tctacgaccg gacgaccccg ccgggagcca
     1921 gccagccccg aggcggcctg agcgacgcgc aggccgcgcg catggccgag gcgatcgaca
     1981 tcctccgcca gcagggcgcc gtcatcgtcg acccggccga cattcccagc atcgtgacgc
     2041 ccgatcggga ccggaacatc ctcttgtggc ccgtctgcgg cggcctcgat caagccaagg
     2101 ggaaggatgc cgactgctcg gtggtgttca agtacgggat gaagcgcgac ttcaacgcct
     2161 ggctggcgtc gctcggagac gcggcgccgg tcaagtcgct gggcgggctg cgcgtctgga
     2221 acgtcgccca ccagcgggcg ggcgccatca agtacggtca ggccctgctg gacgtgtccg
     2281 acgagatgag cctccagggc gaccgcgccc gctaccaggc cgatcgcgag aaggaccttc
     2341 gcctcagcgg ggccgagggc atcgacgccg tcatgaaggc ggagcgcctc gatgccctgc
     2401 tgtttcccgg ttcgagcggg gcgtcgattg cggcgaagcc cgggtatccc accgtgatcg
     2461 tgccgttcgg gttcgttgac gctgccggcg gaggcgccgc ggggggaagg ggcgggcagg
     2521 acttcccgga gggcttccag ccgaagccgt cgcccttcgg cgtcagcttc acgggcatgg
     2581 cctgcagcga gccgaggctc gtcgcgctgg cctacgcctt cgagcaggcc acgaagcggc
     2641 gcgtgccgcc ggggctctga cggccgcgcc ccgcgctacg cgccggtgtc caggcccatc
     2701 tcccgcttga cctcggcgaa ggcgccgatg gcccggtcga ggtccgcccg cgtgtgcgcc
     2761 gccgagatct gggtccggat gcgcgcttcg cccttcggca cgaccgggaa cgagaacccg
     2821 atcacgtaga cgcctttctc cagcatggcg tcggccaccc ggccggcgag cgcggcgtct
     2881 ccgatcatga cggggcagat cggatgcgtg ccaggcagga cgcgaaaccc ggcctgcgac
     2941 atcccttcgc ggaagtagcg cgtgttggag gcgagggcgt cgcgcagcgc ggtggactgg
     3001 ctcagcaggt ccaggacctt gagcgtcgcc cccgcgatgc tcgggcagag cgtgttcgag
     3061 aacaggtacg ggcgcgatcg ctgcctgagc agctcgatga tctcccggcg cccgctcgtg
     3121 tagccgccgc tggcgccgcc gagcgccttg ccgagcgtgc ccgtgaggac gtccacgcgg
     3181 ggcatgacgc cgtggtgctc gtgggtgccg cggccgcgcg cgcccacgaa tcccaccgcg
     3241 tgcgagtcgt ccaccatgac gagggcgccg tggcgctcgg cgagatcgca gatcgccggc
     3301 aggttcgcga tgtagccgtc catcgagaac acgccgtcgg tggcgatcat ccggaagcgc
     3361 gcgtccgcgg aggcgtgcag cttcgcctcc aggtcctgca tgtcgctgtt ggcgtaccgg
     3421 tggcgctgcg cgcggcagag cctgatgccg tcgatgatgc tcgcgtgatt cagcgcgtcg
     3481 ctgatcacgg cgtcccgctc gcccagcagc gtctcgaaga ggccgccgtt ggcgtcgaag
     3541 caggacgaat agaggatcgt gtcctcggtc cccaggaact cgctgagctt ctcctcgagc
     3601 tgcttgtgga ccgcctgcgt gccgcagatg aaccgcgcgc cgcgctggag cgctgggggt
     3661 acggctgcgc nnnnnnnnnn cgcgcgcggc ctcccggacc tccgggtgct gcgccaggcc
     3721 caggtagttg ttggcgcaca ggttgagcac gggcctgccg tccgacaccc ggatcgtcgt
     3781 gccttgcggc gtgaggatga cgcgttcgtc tttgaaggtg ccggcggcgc ggatgtcatc
     3841 gagttgctgg ctggcgaagt gttggtagga accgtacatg aaggcctcgt cgactcgacc
     3901 gccctcgctc cgagggtccc cggttgccag aggcagatcc tattctcgaa cgcgcgcggc
     3961 gaaaagggaa aaggcgccgc cgccggcgcg ccaccggcgc ggcggccccg ggcgcaggga
     4021 tctcgggcag cgcgtgcacg acatcggctc ggcgatcggt tcgaagggag aagcccgagg
     4081 cggagggaag ctcgggcggc cctgcggcag gctcaggacc gccccggatg acggtggtcg
     4141 gggcgactgg atttgaacca gcgaccccct gcgcccaagg caggtgcgct accgggctgc
     4201 gctacgcccc gacccgacgg ccgctccggc gttcggccgg ccggcgcggc cggccgaacg
     4261 ccgcgtgact tcattattat ccccgaacgc gtcgccccgc gctcagacgc cgccgtccat
     4321 ctgaccggaa aggaaggcgt cgtaggccgc catgtcgaag ttgccgtgcc cggagaggtt
     4381 gaacaggatc accttcgcct cgcccgactc ccgggccagc agcgcctgcc gcatcgccgc
     4441 gtgcaccgcg tgggccgatt cgggcgccgg gatggtgccc tcgcagcgcg cgaactgcac
     4501 cgcgctctcg aaaacggagc gctggtcgta ggcctccgcc tcgatcatcc cggcggccag
     4561 caggtggctc acgagcggcg ccgagccgtg gtaccgcagc ccgccgctgt gcaccggcgg
     4621 cggcacgaag ttgtgcccga gcgtgtgcat catcaggagc ggcgtcaggc cggccgtgtc
     4681 gccgaagtcg tactcccgcg gccccttcgt cagcgtcggg cacgacgccg gctccacggc
     4741 gatggcgcgc agcgccggcc ggcgcccctt cagcttgtcc tcgaggaacg ggaacgtcag
     4801 cccggcgaag ttgctgccgc cgccgtggca gccgatcaag atgtccgggt actcgccggc
     4861 cagctccatc tgcttcttcg cttcctggcc gatgatcgtc tggtgcagga tgacgtggtt
     4921 gaggacgctg cccagcgagt acttggtgtc gtcccgcccg gccgcgtcct cgacggcctc
     4981 ggagatcgcg atgcccagcg agcccggcga gtcgggatgc tccgccagga tggcgcgccc
     5041 ggcctgggtc agcggcgagg gactcggcac gatctggccg ccgtagctct gaatcatgat
     5101 ccggcggtag ggcttggtgt cgtagctcac ccgcaccatg tagaccatgc actcgagccc
     5161 catcagccgg cacgcgcacg ccagggccga gccccactgg ccc
//
LOCUS       DAOSIF010000186         5110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14576_length_5110_cov_4.627102, whole genome shotgun sequence.
ACCESSION   DAOSIF010000186 DAOSIF010000000
VERSION     DAOSIF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14576_length_5110_cov_4.627102"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLT35_07440"
     CDS             complement(<1..211)
                     /locus_tag="PLT35_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29096.1"
                     /translation="MSVVGIDIGALFLKAVRLDEAGRVLASVYERHRGEPAGVLAEAF
                     DRLGVQPDEPVGLTGSNGELFALQLK"
     gene            636..887
                     /locus_tag="PLT35_07445"
     CDS             636..887
                     /locus_tag="PLT35_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29097.1"
                     /translation="MLDEPLVRSAIERAPDTWRSAGAISAETGIPLDRVTAVLSASLS
                     DIIVAPGGHDGACDLYSTRTHYRKTKSLLERYLDVLDSS"
     gene            910..1224
                     /locus_tag="PLT35_07450"
     CDS             910..1224
                     /locus_tag="PLT35_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29098.1"
                     /translation="MSPIAAFLWAAGAGFAVSVLDYTALAGVPKRRRRVDFSDPAYLL
                     KFAGHPLVGGLLASAYAASRPDATPLLAAILGAAAPGIWRTIVRSGAAIARALVGRLP
                     AA"
     gene            1403..2491
                     /locus_tag="PLT35_07455"
     CDS             1403..2491
                     /locus_tag="PLT35_07455"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOG29099.1"
                     /translation="MTQVRADDGAPATRPEAQARADRIEAFRQELAALDRAGVLLLGA
                     EDRERVEAYHAGMLDDLAARFDVDRSERQRRMSLGMRVATLLGALTLSAAVVFFFYRV
                     WGLLGTATQVLVLVSAPLVLLAAAEAFARRERTLYVTSIMAVTATAAFVLDLSTAGAI
                     FNLRPSPFAPALWAAFALIVAYRYELRLLLAAGLAMAGWAACAVVGWVAGLDISGWGA
                     RPEPLLPLGALAVAFAASAPNRRRPGFSEVWRLGGLAALLLPLVLLSTWSEAFSYIRL
                     PGKTLGAIYDAAGFALGGAAIWLGFRKGWMRFAQAAAGFLVLFLYVKCFDWLWDWMPR
                     YLFFLLLGGIAVAAVIALGRGRSRRRRL"
     gene            2493..3263
                     /locus_tag="PLT35_07460"
     CDS             2493..3263
                     /locus_tag="PLT35_07460"
                     /inference="COORDINATES: protein motif:HMM:NF027432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4824 family protein"
                     /protein_id="HOG29100.1"
                     /translation="MRGRGLWWASALVVAANLAVLGLSAANRSGEPDAILDLTERELR
                     LPVKEPENTALALSIEFQRRRPGPETESEQPQVKEAGWFDRAKLEAIGFDCSRPVALE
                     YASFYRGQPARTTFAALEYEGDEWRRQVEQAPGPDAAFDTRLIAVDVDNDPAALRRRH
                     PDRRRVAIVEATAILRYVAGAGGPPVLMGRIVGVLPGSITVPREWRAALEAYQSDRLP
                     DSSPPPMREPRYRVTVEWGSRFEPRVTSVEPLVPAAPR"
     gene            3476..4372
                     /locus_tag="PLT35_07465"
     CDS             3476..4372
                     /locus_tag="PLT35_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29101.1"
                     /translation="MTYRPAFAALAVLVAAGGQAPADVRPTAEPRPTVDILVDGRPLP
                     TYHARDTIYVEATKGREYEICIRNPYPVRVAVALSVDGLNTIDARRTSAAAARKWVIE
                     PHGTITIGGWQTSLEHARRFEFTTEERSYGAWLGRANDLGVIAAVFYRERQRQSVAPI
                     WSSSPRQSDAPGTAPQAGGVLSGEAPAPVASERQEEAGARASAANDPSPAGAALARPA
                     AADEYAATGIGRQVQHAVTQVHFELEPSPAASICLRYEYREQLVRLGVLPDTPVHPDA
                     LERRERARGFDPGFCPDPHRSW"
     gene            complement(4415..>5110)
                     /locus_tag="PLT35_07470"
     CDS             complement(4415..>5110)
                     /locus_tag="PLT35_07470"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG29102.1"
                     /translation="PFDPITWDRRRFEALWGWAYRFEAYTPAPQRVRGYYALPLLWGD
                     QVIGWSPSSLMLMFVITNLTASAGSVGFGFLQKWWGNLRTFNLTLVIWIASVAGIWGT
                     PSIAAWMHRAFGVSWSAEHLFLAVGAVAGLCLGATQSASRTIVAVFSPSSKSAEFFGF
                     WGLFGKLASVAGLLSIGALQARFGLRTAILVCSLFFLGALLATVFVREARGRAAAAAF
                     DLGSDPGFRGQIP"
BASE COUNT          707 a         1827 c         1858 g          718 t
ORIGIN      
        1 ccttcagctg gagcgcgaac agctcgccgt tggagccggt caggcccacc ggctcgtccg
       61 gctggacgcc cagccggtcg aacgcttccg ccagcacccc ggccggttcg ccgcggtgcc
      121 gctcgtagac gctggccagg acccggccag cctcgtcgag gcggacggcc ttgaggaaca
      181 gcgctccgat gtcgatgccg accacggaca tgccaggggc tccaattgta ggggatgcgg
      241 taagcgtgac gtcccaaacg gcgccagcac gcggcggcac cgcacccgcc gcgtgcgatc
      301 cctggctcca gtctaccgga cacgtcccgt tcggatctac ccgccgcgcc tcggccgtcc
      361 gggagggcgt taccgagccg gcgccgcggc cgggcggatc cccgctcgag aggcggtcgc
      421 gaccgaggcg cagaaggcgc ctggcccgca tcaccggcgc cggggcggcg ggttgcacgc
      481 tgattgcagc ggggccttga aatcccgttg cacgcgcggg tgaggccgtc tacgatgcgc
      541 gggggaggcg cgtggcgcag ccgtcgaacg gaacgctgcc ggcgaggccg cccgggccgc
      601 gcggccaagg ccgcacatgg gcccggtgga cggccgtgct cgacgagccg ctggtgcggt
      661 cggccatcga gcgcgcgccg gacacctggc gatcggccgg cgcgatcagc gcggagacgg
      721 gcattccgct cgaccgggtg acagccgtcc tcagcgcctc cctgtcggac atcatcgtcg
      781 cgccaggcgg acacgacggc gcctgcgacc tgtactcgac tcgcacgcac taccggaaga
      841 ccaagagcct gctcgagcgc tacctggacg tgctggattc ctcgtaggcg ggacggaggc
      901 tgcgcggcca tgagcccgat tgcggcgttt ctctgggcgg ccggggcggg gttcgccgtc
      961 agcgtcctcg actacacggc actcgccgga gtgccgaaac ggcgccgccg cgtcgacttc
     1021 tccgatcccg cctacctcct caagttcgcg ggccacccgc tggtcggagg gctcctggcc
     1081 tcggcctacg ccgcgagccg gcccgatgcc acgccgctgc ttgccgcgat cctcggggcc
     1141 gcggctcccg gcatctggcg cacgatcgtg cggtcgggcg cggcgatcgc cagggcgctc
     1201 gtcggccgtc tccccgccgc ctgaccgccg ccctcgcccg gggcggcggc cccgcccggc
     1261 tatcccgggc tgagtgagcc cctcctgcgc gagcggacgc atgcccgggc cggagaggca
     1321 ggcgggaggt tgcgtgactc tcccatctga gagtaacatc cgcccgtacc gattcccact
     1381 gaacctgcgg ggcgcccttg ccatgacgca agtccgcgcg gatgatggtg cgccggcgac
     1441 gaggccggag gcgcaggcgc gggccgaccg gatcgaggcg ttccgccagg agttggccgc
     1501 gctggaccgc gccggcgtct tgctcctggg ggccgaggac cgcgagcgcg tggaggcgta
     1561 tcacgcgggc atgctcgacg atcttgcggc ccggttcgac gtggaccgga gcgagcgaca
     1621 gcgccgcatg tcgctgggga tgcgcgtcgc cacgctcctc ggcgccctca cgctgagtgc
     1681 ggccgtcgtc ttcttcttct accgcgtgtg gggactgctg ggcacggcga cgcaggtgtt
     1741 ggtgctcgta tcggcgccgc tggtcctgtt ggccgccgcc gaagcgttcg cgcgccgcga
     1801 gcggaccctc tacgtgacgt cgatcatggc ggtgacggcg acggcggctt tcgtgctcga
     1861 cctcagcact gccggcgcga tcttcaacct gcgcccgtcg ccgttcgcgc ctgcgctctg
     1921 ggcggccttc gcgctgatcg tagcctaccg ctacgagttg cgactgctgc tggcggcagg
     1981 cctcgcgatg gccgggtggg cggcctgcgc ggtcgtcgga tgggtcgccg gactcgacat
     2041 cagcgggtgg ggggcaaggc ccgagccgct gctgccgctc ggcgccctgg ccgtcgcctt
     2101 cgccgcgtcg gcgccgaacc ggcgccgtcc gggattcagc gaggtgtggc gcctcggagg
     2161 cctggccgcg ctgctgcttc cgctcgtcct cctgtccacc tggagcgagg ccttcagcta
     2221 catccggctg cccggaaaga cgctcggcgc gatctacgac gccgccggct tcgccctcgg
     2281 cggcgccgcg atctggctcg gcttccggaa gggctggatg aggttcgctc aggcagcggc
     2341 cgggttcctc gttctgttcc tctacgtcaa gtgcttcgac tggctgtggg actggatgcc
     2401 gcgctacctg ttcttccttc tgctcggcgg gattgccgtg gctgcggtga tcgcgctcgg
     2461 gcgcggccgg tcgcggcggc ggaggctgtg agatgcgagg gcgcggtctg tggtgggcgt
     2521 cggcgctcgt ggtggcagcc aacctggccg tgctggggct gtccgcggcg aatcggtccg
     2581 gcgagcccga cgccatcctc gacctgaccg agcgcgaact ccgcctgccg gtcaaggaac
     2641 ccgagaacac ggcgctcgcg ctgagcatcg agttccagcg tcggcggccc gggccggaga
     2701 cggagtccga acagccgcag gtgaaagagg caggctggtt cgaccgcgcg aaactcgagg
     2761 ccatcgggtt cgattgcagc cggccggtcg cgctggagta cgcctcgttc taccggggcc
     2821 agcctgcgcg cacgacgttc gcggcgctcg aatacgaggg ggacgagtgg cggcggcagg
     2881 tggagcaggc gccgggcccg gacgccgcgt tcgacacgcg cctcatcgcg gttgacgtgg
     2941 acaacgatcc ggctgcgctt cgccggcgcc acccggaccg gcggcgcgtg gccatcgtcg
     3001 aggcgacagc gatcctgcgc tatgtcgccg gcgccggcgg gcccccggtc ctcatggggc
     3061 gcatcgtggg cgtcttgccg ggctcgatca ccgtgccgcg tgagtggcgc gcggcgctcg
     3121 aagcgtacca gagcgatcga ttgccggact cgtcgcctcc gccgatgcgc gagccgcgct
     3181 accgggtcac ggtggagtgg ggatcgcgtt tcgagccgcg ggtgaccagc gtcgagccgc
     3241 tggtcccggc cgcgccgaga tgaccgcccg gccgcctcgc gctgggggtc tgctgccctg
     3301 caacggccag cctccgactt cgcagtaagc ccacgatcta ccccgcgatc gccgcccggc
     3361 tgccccggac atcgcgctcg gctcgtgccc gccgtccgtt caggctggac ccgccgcgac
     3421 ggcgccgggc ctgcaagaaa acgccaggcc gtcgttcttc aggcaggagg acgacatgac
     3481 gtacagaccc gcgtttgcgg ccctagcagt cctggtggca gccggcggcc aggcgccggc
     3541 ggacgtgcgg ccgacggcgg agccgcgccc gaccgtcgac atcctggtcg acggtcggcc
     3601 gctgccgacc taccacgcgc gcgacacgat ctacgtcgag gccacaaagg ggagggagta
     3661 cgagatctgc atccggaatc cctatcccgt gcgcgtggcg gtcgcgctgt cggtcgacgg
     3721 cctgaacacg atcgacgccc gccgcacgag cgctgcggcc gcgcggaagt gggtcatcga
     3781 gccgcacggc acgattacca tcggcggatg gcagacgagc ctggagcacg cgcggcgatt
     3841 cgagttcacg accgaggagc gatcgtacgg cgcgtggctc gggagggcga atgacctcgg
     3901 tgtgatcgcg gctgtcttct accgcgagcg gcagcgacag tccgtcgcgc ccatctggtc
     3961 gtccagcccg cggcagtccg atgcgcctgg cacggcgccg caggcgggcg gcgtcttgag
     4021 cggggaggcg cctgcgccgg tggcgagcga gcggcaggag gaagccggtg cgcgggcgtc
     4081 ggccgcgaac gacccatccc cagcgggagc cgcgctggcg cggccggccg cggccgacga
     4141 gtacgccgcc accggcatcg gacgtcaggt tcaacacgcc gtcacccagg tgcacttcga
     4201 attggagccc tctccagccg cgtctatctg cctccgctac gaataccgcg agcagcttgt
     4261 gcgtttgggg gtgctgcccg acacaccggt ccatcccgac gcgctcgagc gccgcgagcg
     4321 cgcccgcggc ttcgacccgg gcttctgccc ggacccgcac cggtcctggt agcggattgg
     4381 ggtcagatcc aggtttccgg ggtcagatcc aggattacgg gatctgaccc cggaaacctg
     4441 gatctgaccc caagtcgaat gcggcggcgg cggcccgtcc gcgcgcctcg cgcacgaaca
     4501 cggtggcgag cagggccccg aggaagaaca gcgagcagac caggatggcg gtccgcaggc
     4561 cgaagcgcgc ctggagcgct ccaatcgaca acagcccggc caccgaggcg agcttgccga
     4621 acagacccca gaacccgaag aactccgccg acttggagga ggggctgaac acggcgacga
     4681 tcgtccggct cgccgattgc gtcgcgccca ggcacagccc cgccaccgca cccacggcga
     4741 ggaacaggtg ctcggccgac caggacacgc cgaaggcccg gtgcatccag gcggcgatcg
     4801 agggcgtgcc ccagatgccc gccaccgacg cgatccagat cacgagcgtc aagttgaacg
     4861 tgcgcaggtt gccccaccac ttctgcagga acccgaagcc gaccgacccc gcgctggccg
     4921 tcaggttcgt gatcacgaac atcagcatca ggctcgacgg gctccagccg atcacctggt
     4981 cgccccacag cagcggcagc gcgtagtagc cgcgcacgcg ctggggcgcg ggggtgtagg
     5041 cctcgaagcg gtaggcccag ccccacaggg cctcgaagcg gcggcggtcc caagtgatcg
     5101 gatcgaaggg
//
LOCUS       DAOSIF010000187         5090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14649_length_5090_cov_4.201192, whole genome shotgun sequence.
ACCESSION   DAOSIF010000187 DAOSIF010000000
VERSION     DAOSIF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14649_length_5090_cov_4.201192"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLT35_07475"
     CDS             <1..389
                     /locus_tag="PLT35_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29103.1"
                     /translation="PVRPRGVHANLNHEALESLAGCAPEGLSTLVRTAYDTPTIDASL
                     REGVLTLRTRRYAGGFLTSTDLRHFVVAIDKAGPTRLTNPLGPFEAVPDGSSLTIRQA
                     LSGDDARHFIWFVFSGGELRVLTPGR"
     gene            complement(460..1926)
                     /locus_tag="PLT35_07480"
     CDS             complement(460..1926)
                     /locus_tag="PLT35_07480"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOG29104.1"
                     /translation="MIFNSLHFAAFFLVVYAVYRVVPHRGQNWLLLAAGYYFYASWDP
                     RFLGLLIAVTLAAYACGLLLGRTADRGRRKGLLAAGLAFNLTVLGFFKYAGFFAENLQ
                     CLLALAGWRADAVTLRVILPVGISFYTFVAMGYLIDVFRRQAEPARNLRDFAVFIGFF
                     PTLLAGPIMRSSGLLRQIASPRSLAPGAAAEGLWLILWGAFKKMFVADNLARAVDGVF
                     AAGHEPSGLAVVLGTVAFAFQIYGDFSGYTDIARGTSRLLGIDLKVNFRFPYFVTTPQ
                     GFWANWHITLSEWLRDYLFLPLSYAASRRLDGVRWLGQRDDFWIYASATLVTMLAAGL
                     WHGAAWTYVAWGAYQGLLLVAFRFAARRRTRRTAGPRPWRFQWRDAPAALFMFALTCG
                     GWLLFRAASLQQALQFAGRIVTGFAPSWNDAEAVGLPLAVYAGPLLAVHLAEARRGTL
                     DVVRSWPRVWRFAAVVALVYAIVLFGDFEGSEFLYFQF"
     gene            complement(1923..3056)
                     /locus_tag="PLT35_07485"
     CDS             complement(1923..3056)
                     /locus_tag="PLT35_07485"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29105.1"
                     /translation="MGFRQFFRSNVAVLCAVVVVEFALFEAGFRLKGGSEAAPEFQRL
                     FMPDPYIGYRLAPGASARFTTADFDTRITINQAGVRDREVAPKAAGERRIVVLGDSLV
                     MAVQVELEETFTAQLERRLNASAAPPRRFRVINAGVQGYGPVEEYLFHRHVTSALRPD
                     IVILALYPGNDAVEAGVSARRLDDAGGKRPADDGLTAFQRFTQWRRRLMRRSVVVQVV
                     RLRAMTLVGRFGWRPEIDPPLRTFLADAPPEIARGLDVMREAVSRLAVLTAREGARLV
                     VVLLPARFQVDDGDYGRLKEIAARSGKILERDGATRRFKAALAGVGAPVFDALPPLRE
                     AAKTSGVFMQSTAHFTPYGHARLAEIVERFLREAGLVEDGEGR"
     gene            3212..4441
                     /locus_tag="PLT35_07490"
     CDS             3212..4441
                     /locus_tag="PLT35_07490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG29106.1"
                     /translation="MSTLRRLSRGARARLRAVPAISRWHARLRSRAYERTVQAFTRDG
                     SGRANRFPAGIVYEATMRCNLRCEFCYVGDLLNIEGEWRQELPLEAIRRAFPARSGLQ
                     VNLTGGEIFMRKDIMEVLDLFARKRYVCGYLTTNGTIIDDARADALAELARRGFLRHI
                     SVSVDGPGALHDAARGVRGTFERTAAGLRRLQAAAARRSAPLRLSINTTVAAETLGSL
                     HLMADVAREFGVDAIGLNHLMFATPEEAEQTAKIVGAPDGSAIATFVTPDPGLASADV
                     ARQVQRLQGLCREKGLFFDFRPRVWPSIVQHYYTPGAPLLGRCLYPFLHARVGFSGKV
                     YFCPFIRIEVGDLSTSTIEDVWNSPRYVELRRLLLERKLFPVCRRCCKVELSKDPVPG
                     LGAPAARRRTIPLTTIR"
     gene            4451..>5090
                     /locus_tag="PLT35_07495"
     CDS             4451..>5090
                     /locus_tag="PLT35_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29107.1"
                     /translation="MQQWRRSPGRTLAVLAAAVVGSVLAAHAWQAVITGSYLDVVTGV
                     WLTLADDVAHGVFYRGLIGPDGYGGTRYFPLFFSSIGALVRLGAPPVAAGFAVSLASG
                     VLLALGLRRFLLRLGLPEHLAAGLGALSLAPQFVQQTLLAIRSDVLAAALVVWGLAWT
                     LPAFEHERPQRGALRAAALCFVLAAATKATTLYAPGAAVLALAWSGRARASAR"
BASE COUNT          754 a         1856 c         1793 g          687 t
ORIGIN      
        1 ccccggtgcg cccgcggggg gtgcacgcca acctcaatca cgaggcgctg gagtcgctcg
       61 cgggatgcgc gcccgaaggc ctctcgacgc tggtgagaac cgcgtacgac acgccgacaa
      121 tcgacgcctc tcttcgggag ggcgtcctca cgctccggac gcgccggtac gccggcggct
      181 ttctcacctc cacggatctc cggcattttg tcgtggcgat cgacaaggcc gggccgacgc
      241 ggctgacgaa ccccctcggg ccgttcgaag ccgttccgga tggctcttcc ctgacgatcc
      301 gccaggccct gtcaggcgac gacgcccgtc acttcatctg gttcgtgttc agcggcggcg
      361 aactccgggt gctgactccc gggaggtagc gcggacagcg gaccggaccc cgctcgcttt
      421 cgcggatgcc cggggggatg cgatggcgcc cgctgcgcgt cagaactgga agtagaggaa
      481 ctcgctgccc tcgaagtccc cgaagaggac gattgcgtac accagcgcca cgacggcggc
      541 gaagcgccac acacggggcc acgaccggac gacgtcgagc gtgccgcgcc tggcttcggc
      601 gaggtgcacg gccaggagcg gccctgcata gacggcgagc ggcaggccga cggcctcggc
      661 gtcgttccag gacggggcga acccggtgac gatccggccg gcgaactgca gcgcctgctg
      721 cagcgacgcc gcgcggaaga gcagccagcc gccgcacgtc agcgcgaaca tgaacagggc
      781 ggccggcgcg tcgcgccact ggaaccgcca cggccgcggc cccgccgttc gccgcgtccg
      841 gcgcctggca gcgaacctga aagccaccag cagcagcccc tggtacgccc cccaggcgac
      901 gtacgtccag gccgcgccgt gccagagacc ggcggcgagc atcgtcacga gggtcgccga
      961 ggcgtagatc cagaagtcgt cccgctggcc cagccagcgc acgccgtcca gccgccgcga
     1021 cgcggcgtag ctgagcggca ggaacaggta gtcgcgcagc cactctgaga gggtgatgtg
     1081 ccagttcgcc cagaacccct ggggcgtcgt cacgaaatac gggaagcgga agttcacctt
     1141 caggtcgatg ccgaggagcc tggacgtgcc gcgggcgatg tccgtgtagc ccgagaagtc
     1201 gccgtagatc tggaacgcga acgccacggt gcccagcacg acggccagtc ccgacggctc
     1261 gtgccccgcg gcgaacacgc cgtccacggc gcgcgccagg ttgtcggcca cgaacatctt
     1321 cttgaaggcg ccccagagga tcagccagag cccttcggct gccgcgcccg gcgccagcga
     1381 gcggggcgag gcgatctgcc gcagcagccc ggacgatcgc atgatgggcc ccgccaggag
     1441 cgtggggaag aacccgatga agaccgcgaa atcgcggagg ttccgggccg gctcggcttg
     1501 ccggcggaac acgtcgatga ggtagcccat ggccacgaac gtgtagaacg agatgccgac
     1561 cgggaggatc acgcgcaagg tgaccgcgtc ggcgcgccag cccgccaggg cgaggaggca
     1621 ttgcaggttc tcggcgaaga acccggcgta cttgaagaac cccagcacgg tgagattgaa
     1681 agcgaggccc gcggcgagca ggcccttgcg gcggccgcgg tccgcggttc ggccgaggag
     1741 gagcccgcac gcgtaggccg ccagcgtcac ggcgatcagc aggccgagga agcgcggatc
     1801 ccacgaggcg tagaagtagt agccggccgc gagcagcagc cagttctggc cgcggtgcgg
     1861 cacgacccgg tagacggcgt agacgacgag gaagaacgcc gcgaagtgga gcgagttgaa
     1921 gatcaccgcc cctctccgtc ttccacgagc ccggcctcgc gcaggaaccg ctccacgatc
     1981 tcggcgagcc tcgcgtgtcc gtacggcgtg aagtgcgccg tggactgcat gaagacgccc
     2041 gacgtcttcg ccgcctcccg cagcgggggc agcgcgtcga acaccggggc cccgacgccg
     2101 gccagcgcgg ccttgaaccg gcgcgtcgcc ccgtcgcgct ccaggatctt ccccgaccgc
     2161 gcggcgatct ccttcagcct gccgtagtcg ccatcgtcca cctggaaccg cgccggcagc
     2221 agcacgacga cgagcctcgc cccctcgcgg gcggtcagga cggcgaggcg cgacacggct
     2281 tcgcgcatga catcgaggcc cctggcgatc tcgggcgggg cgtcggcgag gaacgtccgg
     2341 agcggaggat cgatctcggg ccgccacccg aagcggccga cgagcgtcat cgcgcgcagc
     2401 cggacgacct gcacgacgac gctccggcgc atgaggcggc ggcgccactg cgtgaagcgc
     2461 tggaacgccg tcagcccgtc gtccgcgggc cgcttgccgc cggcgtcgtc gagccggcgg
     2521 gccgagactc ccgcctcgac ggcgtcgttg ccgggataga gcgcgaggat cacgatgtcg
     2581 ggccgaagcg ccgacgtcac gtgccggtgg aacaggtact cctcgaccgg cccgtatccc
     2641 tgcacgccgg cgttgatcac acggaagcgg cggggcggcg cggccgaggc gttgagccgc
     2701 cgctccagct gcgcggtgaa cgtctcttcg agctccacct gcaccgccat caccagggaa
     2761 tcccccagca cgacgatgcg gcgttcgccg gccgccttgg gcgcgacctc gcggtcccgg
     2821 actcccgcct ggttgatggt gatgcgcgta tcgaaatccg cggtcgtgaa cctggcggac
     2881 gcccctggtg cgagccggta cccgatgtac gggtccggca tgaacaggcg ctggaactcc
     2941 ggtgccgcct ccgacccgcc cttcaggcga aagccggcct cgaacagggc gaactccacg
     3001 acgacgactg cgcagagcac cgcgacgttc gatctgaaga actgcctgaa gcccatgccg
     3061 tggaatggtc cggggaatgc agcccgccga cgacggcgcc accgggcccg cagtgtacca
     3121 cgcgcggccc ctcccaccga ggcgccgccg gccgcgcgtg ccgcccgagt cgcatccggc
     3181 ccgagcttgc aggtacaatt cccggacgga catgagcacc ctgcgtcggc tctctcgagg
     3241 cgcccgcgcg cggctccgcg cggtcccggc cattagccgc tggcacgccc gcctgcgctc
     3301 gcgcgcgtac gagcgcaccg tgcaggcctt cacccgggac ggcagcggcc gcgccaaccg
     3361 ctttccggcc ggcatcgtgt acgaggccac gatgcggtgc aacctgcgct gcgagttctg
     3421 ctacgtcggc gatctgctca acatcgaagg cgagtggagg caggagctgc ccctcgaggc
     3481 gattcgccgg gcgttcccgg cgcgcagcgg cctccaggtc aacctgacgg gcggcgagat
     3541 cttcatgcgc aaggacatca tggaggtcct cgatctcttc gcgcgcaagc ggtatgtctg
     3601 cggctatctg acgacgaacg gcacgatcat cgacgatgcg cgcgcggacg ccctggcgga
     3661 gctggcgcgc cgggggttcc tgcggcacat cagcgtgtcg gtcgacggcc cgggcgccct
     3721 gcacgacgcg gcgcgcggcg tgcggggcac gttcgagcgc accgcggcgg gtctgcgccg
     3781 cctccaggcc gcggcggcgc gccgctcggc tcccctgcgc ctcagcatca acacgaccgt
     3841 cgccgcggaa accctgggtt cgctccacct catggccgac gtggccaggg agttcggcgt
     3901 ggacgccatc ggcctcaacc acctgatgtt cgccacgccc gaggaggccg agcagaccgc
     3961 gaagatcgtc ggcgcgcccg acggctccgc catcgccacc ttcgtcacgc cggacccggg
     4021 cctcgcctcg gccgacgtcg cgcggcaggt gcagcggctg cagggcctgt gccgggagaa
     4081 gggcctgttc ttcgacttca ggccgagggt ctggccatcc atcgtgcagc actactacac
     4141 gcccggcgct ccgctgctcg ggcgctgcct ctatccgttc ctgcacgcgc gcgtcggctt
     4201 cagcggcaag gtgtacttct gcccgttcat ccgcatcgag gtcggcgatc tctcgacctc
     4261 gacgatcgaa gacgtctgga acagcccgcg ctacgtcgag ctgcgccggc tcctgctgga
     4321 gcgaaagctc ttcccggtat gccggcgctg ctgcaaggtc gagctgtcaa aggaccccgt
     4381 gccgggcctc ggcgctcccg ccgcgcggcg ccggacgatc ccgctcacga cgatccgctg
     4441 agggcgcggg atgcagcagt ggcggcgctc acccggccgg acgctggccg tcctggcggc
     4501 ggccgtcgtc ggctcggttc tggcggcgca tgcctggcag gccgtcatca cgggctccta
     4561 cctcgacgtc gtcaccggcg tgtggctgac gctggcggac gatgtcgccc acggcgtgtt
     4621 ctaccgcggc ctgatcggtc ccgacggcta cggaggcact cggtacttcc ctctcttctt
     4681 ctcgtcgatc ggcgccctgg tgcggcttgg agcgcctccg gtggccgccg ggttcgcggt
     4741 gagcctcgcc tcgggggtcc tgctcgccct gggcctccgc cggttcctct tgcggctggg
     4801 gctgcccgag cacctggcgg ctgggctcgg cgcgctcagc ctcgctcccc agttcgtgca
     4861 gcagaccctg cttgcgatca ggagcgacgt gctggcggcc gcgctcgtcg tctggggcct
     4921 cgcgtggacg ctgccggcct tcgagcacga gcggccgcaa cgcggggcgc tgcgcgccgc
     4981 cgcgctgtgc ttcgtgctgg ccgcggcgac gaaggccacg accctgtacg cgccgggcgc
     5041 cgcggtgctc gcgctcgcgt ggtcggggcg agcccgcgcc tcggcccgcc
//
LOCUS       DAOSIF010000188         5078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14701_length_5078_cov_3.740792, whole genome shotgun sequence.
ACCESSION   DAOSIF010000188 DAOSIF010000000
VERSION     DAOSIF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14701_length_5078_cov_3.740792"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..2031
                     /locus_tag="PLT35_07500"
     CDS             211..2031
                     /locus_tag="PLT35_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG29108.1"
                     /translation="MRHATLRRLRLASQLFFFGLFLFLLFRTESPGSIRSASLNVRIP
                     WPVGWFLEADPLVAIATALSTGTLYRGLLWALPFLLLAILVGRAFCGWICPLGSLHHF
                     VSSLKSEAKRGPRLIESNRYKRWQAAKYYLLAAVLAMAVLGSVAGLLLDPISFLVRSM
                     SVAVLPAFNAALRALIDTAYGWPLGGAKAVVNGAAAVLNATVLSFRQPHFHQAFLIGA
                     LFVFVVALNSRVTRFWCRAICPLGALLGVCSRWSLVQMRKDDEKCDNCRRCLLHCQGG
                     DDPIPGAPWRKAECHLCLNCAHDCPTGGIRFAWSTSASPARHTRETPDLRRRALITAA
                     AAGAAMVPLVRSGPGVRTPAGAFLIRPPGSLDEPHFLDRCIRCGDCMKVCPTNALHPA
                     AFEGGVEGLWTPILVPRIGYCEPNCVLCSQVCPTGAIWEITVAEKTGTRDSGLGTRGA
                     RGQANAAATAPTPDAGTENAASPHADARSADAPETNAQASSGPAADGHPIRIGTAFYD
                     RGRCLPWAMATECIVCEEWCPTSPKAIHLVPADVPGRDGGTKSVKQPYIDPRLCTGCG
                     ACEYACPIKGSPAVYVTSAGETRSRANQFLLPENRQPSRS"
     gene            2031..3284
                     /locus_tag="PLT35_07505"
     CDS             2031..3284
                     /locus_tag="PLT35_07505"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG29109.1"
                     /translation="MQKPRRYQPMLTSSEVTRREFVRAGAASAVVGGIVLGVPSFAAS
                     DQDRTPPPAPETNINDFMKVPKGKHALPGPFPGRVVEVKDARSLADDAVDGKVVAEMF
                     ERGVRTLTGKDMSQSFALLFEPGDVVGIKVNPVGPPLIHTKVELAQAVVRWLMDNKVP
                     AGRIVIWDRFDYMLKEAGYTPDRFPKGVRIEGLQTMDETGNGWRDAAGNHVSTDRFDK
                     DAYYFAKGIVGRNVRGYKNDEFYLNQHVFNGEYSYFGKLLTKDVTRLVNLAAYKNTGA
                     GISMATKNLGYGAVCNTGRLHAPLFFTVCTEVLAAPAVREKLVLNVIDGLRGQYDGGP
                     DKNAQFVYPNHSLYFATDPFALDMCCHRQLMAKRKEMGIAVNEHPRFTEYLHYAERLG
                     LGVANPEKIDYVRVQGPETGSWAGR"
     gene            3359..4255
                     /locus_tag="PLT35_07510"
     CDS             3359..4255
                     /locus_tag="PLT35_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29110.1"
                     /translation="MRSRRKWVRTALTAAALIVAASGALVAQRPTGDAGLMPADAFAG
                     GFKAVGPLRVFTSADLYGHIDGGAEAFLEIGFEQLTVQRYKDGAHEITIDLYRMSDPT
                     AAHGIYIARCGKETPDPAVKARHTASRHQILLQKHRYYVQVNNSAGGAANAPNLVKAA
                     QAVAARLPADAPIAALALLPKSGLVAGSARLFRGPIGLQAVYTLGDGDILRLGGKLTA
                     AAGDYKDAGGAYSLVVAEYPTPAAASSAFRHLTSNLDPYLKPSSSTDTRLVFVDYDKK
                     YGVASVAGNRLEVRLHLAKPPA"
     gene            4594..>5078
                     /locus_tag="PLT35_07515"
     CDS             4594..>5078
                     /locus_tag="PLT35_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29111.1"
                     /translation="MSDKKSWAATVLGWFVVSEDGERREDAAASGGGAAPMPPVFVKD
                     PPAALGGQVDFGAVFEAAGLDADQIGRVSKAQELVSSLPAETPAAVKKQIVEAALTAF
                     GVDIDKIIESGVAEIRALEGYIRTGAAETQALLAESAERIGKFEDEIRRIRTVMDQRV
                     Q"
BASE COUNT          766 a         1809 c         1781 g          722 t
ORIGIN      
        1 cctcgtccgc caccggtatg cgcgccctaa cggcgatcag gctcatgcgg tcgcaccacc
       61 agcccgccgc gtgctcgcaa gccgggcctc ccggcttcta gcgtacctca ccacggggcc
      121 cggcggcacc accggctgcc acggcgtgtg gcgcgattga tccgccgccg cccggggacg
      181 ggggcctcat gcggtatcat gggcgccgac atgcgacacg cgacgctgcg gcgcctccgc
      241 ctggcctcgc agctcttctt cttcggcctc ttcctctttc tgctgttccg cacggagtcc
      301 cccggctcca tcaggtcggc gtcgctcaac gtgcgcatcc cgtggcccgt cggctggttc
      361 ctcgaggcgg atcccctggt cgccatcgcg acggcgctct cgacggggac gctctaccgt
      421 ggcctcctgt gggcgctgcc cttcctgctg ctcgcgatcc tggtcggccg ggcgttctgc
      481 ggctggatct gcccgctcgg atcgctgcac cacttcgtca gcagcctgaa gtcggaggcg
      541 aaacgagggc cgagactcat cgagtcgaac cgctacaagc ggtggcaggc ggcgaagtac
      601 tacctgctcg cggcggtgct cgccatggcg gttctcggct cggtggcggg cctgctgctg
      661 gatccgatct cgttcctcgt gcgctcgatg tccgtggccg tgctgccggc cttcaacgcg
      721 gccctccgcg ccctcatcga cacggcgtac gggtggccgc tcggcggcgc caaggctgtc
      781 gtgaacggcg cggccgcggt cctgaatgcg acggtcctca gcttccggca gccgcatttc
      841 caccaggcgt tcctcatcgg cgcgctcttc gtgttcgtcg tcgcgctcaa ctcgcgcgtg
      901 acgcgcttct ggtgccgcgc gatctgcccg ctcggcgccc tgctgggcgt ctgctcgcgc
      961 tggtcgctgg tgcagatgcg caaggacgac gagaagtgcg acaactgccg gcgctgcctg
     1021 ctccactgcc agggcggcga cgacccgatt ccgggcgcgc cctggcgcaa ggcggaatgc
     1081 cacctctgcc tgaactgcgc gcacgattgc ccgaccggcg gcatccgctt cgcctggtcc
     1141 acgtcggcct cgccggcccg gcacacgcgc gagacgcccg acctcaggcg ccgcgcgctg
     1201 atcacggcgg ccgccgcggg ggccgcgatg gtgccgctcg tgcgctccgg cccgggcgtc
     1261 aggacgcccg ccggcgcctt cctcatccgc cctccgggct ccctcgacga gccccatttc
     1321 ctcgatcggt gcatccgctg cggcgactgc atgaaggtgt gccccacgaa cgcgctgcat
     1381 cccgccgcct tcgagggcgg cgtcgagggc ctctggacgc cgatcctcgt gccgcgcatc
     1441 ggctactgcg agccgaactg cgtcctctgc tcgcaggtct gccccaccgg cgcgatctgg
     1501 gagatcacgg tggcggagaa gaccgggact cgggattcgg gactcgggac tcggggagca
     1561 cggggtcaag ccaacgcggc ggccaccgca ccgacgccgg atgccggcac ggagaacgca
     1621 gcgtcgccgc acgccgacgc ccgatccgca gacgcgcccg agaccaatgc gcaggcgtcg
     1681 tccggacccg cggccgacgg acacccgatc cgcatcggca cggccttcta cgatcgcggg
     1741 cgctgcctcc cgtgggcgat ggcgaccgag tgcatcgtct gcgaggagtg gtgcccgacc
     1801 tcgccgaagg ccatccacct cgtcccggcg gacgtccccg ggcgcgatgg cggaacgaag
     1861 agcgtgaagc agccgtatat cgatccgagg ctctgcacgg gctgcggcgc gtgcgagtac
     1921 gcctgcccca tcaagggcag cccggccgtc tacgtcacca gcgccggcga gacgcgctcg
     1981 cgcgcgaacc agttcctgct cccggagaac cggcagccct cgaggtcgtg atgcagaagc
     2041 cccgccgcta ccagccgatg ctgacgtcct ccgaggtcac gcgccgcgag ttcgtgaggg
     2101 ccggcgccgc ctccgccgtc gtcgggggca tcgtcctcgg cgtaccgtcc ttcgccgcca
     2161 gcgaccagga ccgcacgccg ccgcccgcac cggaaaccaa catcaacgac ttcatgaagg
     2221 tcccgaaggg caagcacgcg ctgccggggc cgttcccggg gcgtgtggtc gaggtgaagg
     2281 acgcccggtc gcttgccgac gacgcggtcg acggcaaggt cgtcgccgag atgttcgagc
     2341 ggggcgtgcg gacgctcacc ggcaaggaca tgtcgcagag cttcgccttg ctcttcgaac
     2401 ccggcgacgt cgtcggcatc aaggtcaacc cggtggggcc gccgctcatc cacacgaagg
     2461 tcgaactggc gcaggcggtg gtgcggtggc tgatggacaa caaggtcccc gccggccgga
     2521 tcgtcatctg ggaccgcttc gactacatgc tgaaggaggc gggctacacg ccggaccgct
     2581 tcccgaaagg cgtccgaatc gagggcctgc agacgatgga cgagaccggc aacggctgga
     2641 gggacgccgc ggggaaccac gtcagcaccg accggttcga caaggatgct tactacttcg
     2701 cgaagggcat cgtcggcagg aacgtgcggg gctacaagaa cgacgagttc tacctgaacc
     2761 agcacgtgtt caacggagag tactcgtact tcggcaagct gctgacgaag gacgtcacgc
     2821 gcctggtcaa cctcgcggcc tacaagaaca ccggcgccgg catctcgatg gcgacgaaga
     2881 acctgggata cggagccgtg tgcaacacgg ggcggctgca cgcgccgctc ttcttcaccg
     2941 tctgcacgga agtgctggcg gcgcccgccg tccgcgagaa gctggtcctg aacgtgatcg
     3001 acggcctgcg gggacagtac gacggcgggc ccgacaagaa cgcgcagttc gtctacccca
     3061 accactcgct gtacttcgcg acggatccgt tcgccctcga catgtgctgc caccggcagc
     3121 tgatggcgaa gcgcaaggag atgggcatcg cggtgaacga gcacccccgg ttcaccgagt
     3181 acctgcacta cgcggagcgg ctgggcctcg gcgtcgcgaa tccggagaag atcgactacg
     3241 tcagggttca ggggccggag actgggagct gggccgggcg ctaggcgctg gtgtgccgcg
     3301 cccgggcccc gacgcgccgg gacgagccgg gacgcaacga gcagagggac agggagcgat
     3361 gaggtcacga cgcaagtggg ttcggacggc gttgacggcg gccgcgctca tcgtggcggc
     3421 gtcgggagcg ctggtcgcgc agcggccgac cggagacgcg gggctcatgc cggccgacgc
     3481 gttcgcgggc ggcttcaagg ccgtcggccc gctcagggtg ttcacgagcg cggatctcta
     3541 cggccacatc gacggcggcg cggaggcctt cctggagatc gggttcgagc agctgaccgt
     3601 gcagaggtac aaggacgggg cgcacgagat caccatcgac ctctaccgga tgtccgaccc
     3661 gacggccgcg cacggcatct acatcgccag gtgcgggaag gagacaccgg acccggccgt
     3721 gaaggcccgg cacacggcga gccgccatca gatcctgctg cagaagcacc gctactacgt
     3781 gcaggtcaac aactcggccg gcggcgcggc caacgcgccg aacctcgtta aggccgcgca
     3841 ggccgtcgcc gccagactgc cggccgacgc gccgatcgcc gccctcgcgc tgcttccgaa
     3901 atcgggcctc gtggcaggct cggcgcggct gttccgcggg ccgatcggcc tgcaggcggt
     3961 ctacacgctc ggcgacggcg acatcctgcg cctcggcgga aagctcaccg ccgcggcggg
     4021 cgactacaag gacgcgggcg gcgcgtactc gctggtggtc gcggagtacc cgacgccggc
     4081 agcggcctcc tcggcgttca ggcacctgac gtcgaacctc gacccctacc tgaagccgtc
     4141 cagctcgacc gacaccaggc tggtgttcgt ggactacgac aagaagtacg gcgtggcctc
     4201 ggtggcgggc aatcgcctcg aggtgcggct ccaccttgcc aagccgcccg cctgacgggg
     4261 ccgccggctc gcgggccctg ccgccggtca cccccgtccg tgctcgcgcg cacagccctt
     4321 gtcacgggag tcgcgctgcg gccggccggg gagcccccga ccggtgtcac ccgctacccg
     4381 gcggggttcc tgagcgtggg cggcggcacg gcgtgccgcg aaccgctctt gccctcgccc
     4441 agtcccacga gcacctagcg cccaacctct ggcccctgga gagagccccc atttgccgcc
     4501 ggagcgccgc agtcgtacaa ttccgtccca ataccgcggg agcgtctccc ccgcatgacc
     4561 gggtcggcgc atgaggcgcc ggagcgagcc gagatgagcg acaagaagag ctgggccgcg
     4621 acggtcctcg gatggttcgt cgtgtcggag gacggggagc ggcgggagga tgccgcggcc
     4681 tccggcggcg gggcggcgcc catgccgccg gtgttcgtca aggacccgcc ggccgctctg
     4741 ggcggccagg tggacttcgg cgcggtcttc gaagccgcgg gcctggacgc ggatcagatc
     4801 ggccgcgtgt cgaaagcgca ggaacttgtg tccagcctgc cggccgagac gccggcggcg
     4861 gtcaagaagc agatcgtcga ggcggcgctc accgcgttcg gcgtggacat cgacaagatc
     4921 atcgagagcg gcgtggccga gatccgcgcg ctcgaaggct acatccggac tggggcggcc
     4981 gagacccagg cgctgctggc ggagtcggcc gagcgcatcg ggaagttcga ggacgagatc
     5041 cggcggatcc ggacggtgat ggaccagcgg gtgcagga
//
LOCUS       DAOSIF010000189         5077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14710_length_5077_cov_3.284150, whole genome shotgun sequence.
ACCESSION   DAOSIF010000189 DAOSIF010000000
VERSION     DAOSIF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14710_length_5077_cov_3.284150"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLT35_07520"
     CDS             complement(<1..481)
                     /locus_tag="PLT35_07520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG29112.1"
                     /translation="MMVPHTRHAHAPLCALAVALAVALVAAAPPHAAAQTTAAAPPVV
                     RSYVAPADVAAPPVDAVKTASGLATKVLVPGTGTAHPKADDFVSVHYIGWTAQGKMFD
                     SSYPRGKAASFQLPRVIPGWSEAVQLMVVGEKRRAWIPEALAYKGRKDKPQGMLVFDI
                     "
     gene            complement(478..1962)
                     /locus_tag="PLT35_07525"
     CDS             complement(478..1962)
                     /locus_tag="PLT35_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOG29113.1"
                     /translation="MPISGDALKIAVFIDFDNIEIGVKTTLNREFEVGAVLDAVKERG
                     EVITKVAYGDWKRAGDYSRMLTQHAVRMVQRNMTPGGDKNGADINLALDALEMAFTHD
                     HINAFVIVGGDSDFISLVEKLKQYDRKVFVVGGRQFTGAVMQKNCHEFIAYENLIRQT
                     GRRPAGHGRERGGEKAAPLATAPVSRAVPLVTRALALLADREVTPQLGLLKSTLLQLD
                     STFSERDYGASSFRDFAEKLAETGVVALRSSGRSVLVELKDAERVHDAPEGHPAEADA
                     AAPSPAAAAPAAEPEAQPPGHDEAPPTSIVDGIRMLHRVIATPGAITRWPMYVRNVKQ
                     AMRAVEPGFDERRHGNLVDILRAVQRDGLVRLERDRQGVLRVFQGQLPRVPAAQPAEP
                     APEPVEAAPSASAQPADLLLSPDGREPADASLEPRGGAESSEPVAPDSRERTAEEAPA
                     ADATPRRRRRSGSAVPKAKVTAARKPRARSTSSRTKKDKDTEPS"
     gene            complement(1979..3883)
                     /locus_tag="PLT35_07530"
     CDS             complement(1979..3883)
                     /locus_tag="PLT35_07530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOG29114.1"
                     /translation="MPSYTAKDITVLEGLEPVRKRPGMYIGGVGSTGLHHLAWEILDN
                     AVDEAMNGFASAITVTLHEDGASLTVSDDGRGIPIDKHPKSRKSALEVVFTVLHAGGK
                     FEHGSYKTAGGLHGVGASVVCALSKELSVTVKRDGEQWEQRFKQGVAQGPPKKLGPAR
                     GTGTTVFFRPDATIFPKVEFDAAVIRERLEVASYLHKGVKVVFEDEGARTREVFQHDD
                     GIEAYLRAILAAAGARPSHESAFVLARDNGMRLDLAMQWTEGTDEQIRSYVNGIPTGS
                     GGTHEHGLRAGVGKAIRNFIDTHGLTPKGVTIAAEDIREGLVAVLSLFVAEPQFQGQT
                     KDRLNNPEALSAVDSAVRPALEHWLNHNLSMAEAIVARIILAARAREASRAAQQEVAR
                     KNATSNRLNLPGKLSDCSAAGRDDSELFIVEGDSAGGSAKQGRDRARQAILPLRGKVL
                     NVESASVSKVLENKELSDLVTALGCGIGKHVDITRLRYGRVIILADADSDGNHIATLL
                     LTFLYRHLTPLMTAGKVFLAQPPLYRIDIGKETHWALDEIQKAEILERHAKGRGTPEI
                     TRFKGLGEMMPKVLWETTLNPKTRRLLRVEVADHIVTDRVINELMGKDASARFRFIME
                     RAEEARELDV"
     gene            4225..>5077
                     /locus_tag="PLT35_07535"
     CDS             4225..>5077
                     /locus_tag="PLT35_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG29115.1"
                     /translation="MATFTRRQPDRTPAVRRAFQMAFILLNLWIGATFYLFVRYYETG
                     GQSIQVNRPPGVEGWLPIAGLMNLKYAILTGRIPEVHPAAMFLLAAFIGISFLARKAF
                     CSWLCPIGTISEWLWQGGREIFGRNVQAPRGLDIPLRSFKYLLLGFFGYAVASMTAED
                     LRAFVFSPYGQIADVKMLNFFRHIGTTPAIVIAMLIVGSLAVKNLWCRYLCPYGALVG
                     IAALFSPARIRREPDVCIDCEKCAKACPSLLPVDRLITVRSAECTGCLECIASCPAQG
                     ALRLAVGR"
BASE COUNT          689 a         1759 c         1747 g          882 t
ORIGIN      
        1 cgatgtcgaa caccagcatg ccctgcggtt tgtccttccg gcccttgtac gccagggctt
       61 cgggaatcca ggcgcgccgc ttctcgccga ccaccatcaa ctggacggcc tcgctccacc
      121 ccggaatgac gcgcgggagc tggaacgatg ccgccttgcc gcgcgggtag gagctgtcga
      181 acatcttgcc ctgcgccgtc cagccgatgt agtggacgct cacgaagtca tcagccttcg
      241 ggtgggccgt cccggtgccc ggcacgagca ctttcgtggc cagcccggag gcggtcttga
      301 ccgcatcgac gggcggagcg gcgacgtcgg ccggggcgac gtacgatctc acgaccggcg
      361 gcgcggcggc ggtcgtctga gcggccgcgt gcggcggcgc ggcagcgacg agcgcgacgg
      421 cgagcgcgac ggcgagcgcg cacaacgggg cgtgagcgtg gcgggtgtgc ggtaccatca
      481 tgagggttcc gtgtccttgt ccttcttcgt gcgtgacgat gtggagcgcg cccgcggctt
      541 gcgggcggcc gtgaccttgg ccttggggac tgccgaaccg gacctgcgcc ggcggcgcgg
      601 cgtagcatcg gcggcgggag cctcctccgc cgtgcgttcg cgtgaatcgg gagcgacggg
      661 ttcggaggat tccgcgccgc cgcgcggctc gaggctggcg tcagcgggct cgcgcccgtc
      721 gggcgagagg agcagatcgg ccggctgcgc cgaagccgag ggagccgcct cgacgggctc
      781 gggcgccggt tcggccggct gcgccgcggg gacgcgcggc agctgcccct ggaacacccg
      841 cagcacgccc tgccgatcgc gttcgaggcg caccaggccg tcgcgctgga ccgcccggag
      901 gatgtcgacg aggttgccgt ggcggcgctc gtcgaagccg ggttccacgg cgcgcatggc
      961 ctgcttgacg ttgcgcacgt acatcggcca ccgcgtgatg gcccccggcg tggcgatcac
     1021 ccggtggagc atccggatgc cgtcgacgat cgacgtcggc ggcgcctcgt cgtggccggg
     1081 tggctgcgcc tccggctcgg cggctggcgc ggctgccgcc ggggacggcg cagcggcgtc
     1141 cgcctcggcc gggtggccct ccggggcgtc gtggacgcgt tctgcgtcct tcaactccac
     1201 gagcacgctc cgtcccgagc tgcgcaatgc gacgacgccg gtctcggcga gcttctcggc
     1261 gaaatcgcgg aagctcgagg cgccgtagtc ccgctccgag aacgtcgagt cgagctgcag
     1321 cagcgtgctc ttcagcagcc cgagctgcgg cgtgacctcg cggtccgcca ggagcgcgag
     1381 cgcccgcgtc acaagcggga cggcccggct gacgggagcg gtggccagcg gggccgcttt
     1441 ctcgcccccg cgctcgcggc catggccggc cggccgccgg cccgtctgcc ggatgaggtt
     1501 ctcgtaggcg atgaattcgt ggcagttctt ctgcatcacg gcgcccgtga actgccgtcc
     1561 gcccacgacg aacaccttcc tgtcgtactg cttgagcttc tccaccaggc tgatgaagtc
     1621 gctgtcgccg ccgacgatga cgaaggcgtt gatgtggtcg tgggtgaagg ccatctcgag
     1681 ggcgtcgagg gcgaggttga tgtcggcgcc gttcttgtcg ccgccaggcg tcatgttgcg
     1741 ctgcaccatc cgcaccgcgt gctgcgtgag catgcggctg tagtcgccgg cgcgcttcca
     1801 gtcgccgtag gcgaccttcg taatcacctc tccgcgctct ttgacggcgt ccagtacggc
     1861 gccgacctcg aactcgcggt tcagcgtggt cttcacgccg atctcgatgt tgtcgaaatc
     1921 gatgaagacc gcaatcttca gggcatctcc tgatatgggc atggatcctt cttgatggtc
     1981 acacgtccag ctcgcgggcc tcctcggcgc gttccatgat gaagcggaac cgcgcggaag
     2041 cgtccttgcc catgagctcg ttgatgacgc gatcggtcac gatgtggtcg gccacctcca
     2101 cgcggaggag ccgccgggtc ttcgggttga gggtggtctc ccacagcacc ttcggcatca
     2161 tctcgcccag gcccttgaag cgcgtgatct ccggcgtgcc gcgccccttg gcgtggcgct
     2221 ccaggatctc cgccttctgg atctcgtcga gggcccagtg cgtctccttg ccgatgtcga
     2281 tgcggtagag gggcggctgg gcgaggaaga cctttcccgc cgtcatgagc ggggtcaggt
     2341 gccggtagag gaaggtgagc aggagcgtgg cgatgtggtt cccgtccgaa tccgcgtcgg
     2401 ccaggatgat cacgcggccg tagcgcagcc gcgtgatgtc gacgtgcttg ccgatgccgc
     2461 agccgagggc ggtgacgagg tcggacagct ccttgttctc cagcaccttc gagaccgagg
     2521 cgctctcgac gttcaggacc ttgccgcgga gcggcaggat cgcctgccgc gcgcggtcac
     2581 ggccctgctt cgccgacccg ccggccgagt cgccctccac gatgaagagc tccgagtcgt
     2641 cgcgccccgc cgcggagcag tcgctgagct tgccggggag gttcaggcgg ttcgacgtcg
     2701 cgttcttgcg tgcgacctcc tgctgcgctg cgcggctggc ttcccgggcg cgcgcggcga
     2761 ggatgatgcg cgcgacgatg gcctctgcca tgctgaggtt gtggttcagc cagtgctcga
     2821 gggccggccg caccgccgag tccacggcgg acagcgcctc cgggttgttc agccggtcct
     2881 tcgtttgccc ctggaactgc ggctcggcca caaagaggct cagcaccgcg accaggcctt
     2941 cgcggatgtc ctcggccgcg atcgtcacgc ccttgggggt caggccgtgc gtgtcgatga
     3001 agttccggat ggctttgccg acgcccgccc tgaggccatg ctcgtgggtc ccgcccgagc
     3061 cggtcgggat cccgttgacg tagctccgga tctgctcgtc ggtgccctcc gtccactgca
     3121 tcgccagatc caggcgcatc ccgttgtcgc gggcgagcac gaaggccgac tcgtgcgagg
     3181 gcctggcgcc cgccgccgcc aggatcgccc tgaggtaggc ctcgatgccg tcgtcgtgct
     3241 ggaagacctc gcgggtcctc gcgccttcgt cctcgaagac gaccttgacg cccttgtgca
     3301 ggtagctggc gacctcgagg cgctcgcgga tgacggcggc gtcgaactcg actttcggga
     3361 agatcgttgc gtccggcctg aagaagaccg tcgtgcccgt gccccgcgcc ggccccagct
     3421 tcttcggcgg cccctgggcc acgccctgct tgaagcgctg ctcccactgc tcgccgtctc
     3481 gcttgaccgt gacggacagc tccttcgaca gtgcgcagac cacgctggcg ccgaccccgt
     3541 gcaggccgcc cgccgtcttg tagctcccgt gctcgaactt gccgccggcg tgcagcacgg
     3601 tgaagacgac ctccagggcg ctcttcctgc tcttcgggtg cttgtcgatg gggatgccgc
     3661 gcccgtcgtc cgagaccgtc agcgaagcgc catcctcgtg cagcgtcacc gtaatggccg
     3721 aagcgaagcc attcatcgct tcgtccacgg cgttgtccag gatctcccag gccaggtggt
     3781 ggaggccggt cgagcccacg cccccgatgt acatgccggg gcgtttccga acaggctcga
     3841 ggccttcgag gacggtgatg tccttggctg tataggacgg catcaacgga acacgcgcta
     3901 gggactaggc gccaggcgct agggacagcc ggcgcacagg ggcgcgtcgg ccgctaggga
     3961 ctgcgctgaa gagatgccag cgttccgcgc cccgaaacaa gccccccggg acagccggga
     4021 cgagccagga gcatggaccg ctcgccaatt ccttgacgcc attgtacttc gattcgtcgt
     4081 cgtaccgcca cctcggcttg gggtcagatc caggaatacg gggtcagggc cagtattact
     4141 ggccctgacc ccgtattcct ggatctgacc ccgatacccg acagcagccg gcgccgcctg
     4201 gcccgtgcct gatattctgg actcatggcc acgttcaccc gccggcagcc cgaccggacg
     4261 ccggccgtcc gccgcgcgtt ccagatggcc ttcatcctgc tcaacctctg gattggcgcg
     4321 accttctacc tcttcgtccg ctactacgag acgggcggcc agagcatcca agtcaaccgg
     4381 ccgccaggcg tcgagggctg gctgccgatt gcgggcctga tgaacctcaa gtacgccatc
     4441 ctgaccggcc gcatccccga ggtgcacccg gcggcaatgt tcctcctcgc cgcgttcatc
     4501 ggcatttcct tcctcgcgcg caaggcgttc tgcagctggc tctgcccgat tggcacgatc
     4561 tccgagtggc tgtggcaggg cgggcgcgag atcttcgggc gcaacgtcca ggctccgcgc
     4621 gggctcgaca tcccgctccg aagcttcaag tacctcctgc tcgggttctt cggctacgcc
     4681 gtcgcaagca tgacggcgga ggacctgcgg gccttcgtct tcagccccta cggccagatc
     4741 gccgacgtca aaatgctgaa cttcttccgg cacatcggga cgacgccggc catcgtcatt
     4801 gcgatgctca tcgtaggctc gctggccgtg aagaacctct ggtgccgcta cttgtgcccc
     4861 tacggggcgc tggtcggcat cgccgccctc ttcagcccgg cccggatccg acgggaaccg
     4921 gacgtctgca tcgactgcga gaagtgcgcg aaggcgtgcc cgtcgctgct gccggtggat
     4981 cggctgatca ccgtcaggtc ggccgagtgc accggctgtc tggaatgcat cgcgtcgtgc
     5041 ccggcccagg gggcgctccg cctagcggtg ggccgat
//
LOCUS       DAOSIF010000190         5075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14718_length_5075_cov_4.073506, whole genome shotgun sequence.
ACCESSION   DAOSIF010000190 DAOSIF010000000
VERSION     DAOSIF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14718_length_5075_cov_4.073506"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLT35_07540"
     CDS             complement(<1..692)
                     /locus_tag="PLT35_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG29116.1"
                     /translation="MTSRPLPPAGPAGLVPGRPWALLWLMAGFNVLNFVDRQLVGSLA
                     PLLMADLQISRAQIGLLAGFAFVLCYTVAGMAIGIAADRWPRRAIVLGGVWLWSLMTA
                     VSGAARGFADLAAARVFLGVGQAALTPAALPMLADALPSRRWGLATGIYYAGLPVGTA
                     LSLIVAGFVAPRFGWRACFYALGALGIAASSLLALVREPVRRQGAPPAAGRASGQSGA
                     AQAFGATARELFR"
     gene            800..1588
                     /locus_tag="PLT35_07545"
     CDS             800..1588
                     /locus_tag="PLT35_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29117.1"
                     /translation="MSEFQKHLYLILHPNEALVASQLSPEAFGAHYSIGSPRHFTGKV
                     IFAEVDISFRHPDLRIDEYLAQTESGQSGRPKRTKFVKSYRVLEHIDLTAFQRLYLVT
                     TDGAVLGLDPGDEPADTRRAGRIRIYQEICPLRLVVASSLEPRAFGAYITAETWSKGA
                     PKIFFAQWDVDAEAIVNRLDVLSHSGQLPNVNPANLSNALRELAEIPGKKSKTLSLNG
                     NLDLTSFKNIQPGFYFSEGDKLLFYRMPTMEELHDKHRAWWRHL"
     gene            complement(1874..3025)
                     /locus_tag="PLT35_07550"
     CDS             complement(1874..3025)
                     /locus_tag="PLT35_07550"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HOG29118.1"
                     /translation="MTYDFDRPVDRIGSDSTKWRVYGPGVLPLWTADMDFRVPEPVIA
                     ALRARVDHGVFGYCVEPPELREILAERLGRLYGWHVSAGDIVLGTGVVTAFHRACRAA
                     AGPTDGILVQTPVYQPILGAAAHNGSVGQQAPLARRADGRWEIDFEAFEAAVTGRTRV
                     FILCNPHNPVGRVFSRADLERLASMCLRRDVLICSDEIHCDLVFPGSAHTPIASLDPE
                     VAKRCVTLMAPSKTFNVPGLRCSFAVVTDPDLRRRLVGEESATFSEVNNLALAATLAA
                     YRDGQDWLDQVLAYLAANRDFVAEFVRRELPGAAMAPPEATYLAWLDCRTCGIDGNPQ
                     QFFLERARVALQDGEWFGPGGEGFVRLNFGCTRATLAEALERMATALRE"
     gene            3198..4754
                     /locus_tag="PLT35_07555"
     CDS             3198..4754
                     /locus_tag="PLT35_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29119.1"
                     /translation="MPSEMNRREFLSGAAAAAASFTIVPRRVLGGAGFVAPSDKITLA
                     CIGFGTQAIREIGGILASPDVEVVAVCDVDKTGVGYLEWGKDQIRNDIRRIIDEPTWR
                     ENVKGVPGGLDVGKEIVDTYYRKQRGAAPGAKGCAAYVDYRELLEQEKDVTAVKVLTP
                     DHTHAPIAIAALRRGMNVIVHKPLANRVLEARAVIEAARAANKATHFLPASEGASQRQ
                     ALEMIGNGAIGTLREIHNWSARPMWPQFPTLPADTPPVPAGFDWTLWLGPSLDRPYHP
                     NYTHTNFRGWYEFGGGSIADMGHYSLWPIFQALELDAPLSVESTPSHVCAVADDICYT
                     IKNDYSFPAACTVRMRFGAKRARPGLDIFWYDGGIRPPVPEELMAENKELPSEGMLFV
                     GDTGKILGGFRAENPQIVPEAKMRAYRAAHNIPEPAPPQPRQRGPQDLRPRPSERDLA
                     WINAFKGGPRSYGDFLLAQPICDAVNLAAVSLRLGGRRLLWDAAAARVTNVPEANALL
                     TREYRPGWQI"
     gene            4767..>5075
                     /locus_tag="PLT35_07560"
     CDS             4767..>5075
                     /locus_tag="PLT35_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29120.1"
                     /translation="MTGLTRREFIERTGAAAGFLAAGMRGLAAAPLGLPIGSQVYPMR
                     HLLKDFPAFVKAMAGIGVTRLELCSPIGYGTEFQPLSNGREVRAILADHGLKAESSHF
                     S"
BASE COUNT          827 a         1791 c         1789 g          668 t
ORIGIN      
        1 cggaacaact cccgcgcggt cgcgccgaag gcctgggcgg cgcccgactg cccggaggcg
       61 cggccggccg ccgggggtgc gccctggcgc cgaaccggtt cgcggacaag cgccagcagc
      121 gacgaggctg cgatgccaag cgcaccgagc gcatagaagc acgcccgcca cccgaaccgc
      181 ggggcgacga agcccgcgac gatcagactg agggccgtgc cgacgggcag cccggcatag
      241 taaatcccgg tggccagccc ccatcgcctc gacgggagcg cgtcggcaag catcggcagc
      301 gccgccggcg tcagcgccgc ctggccgacg ccgaggaaca cccgggcggc tgcgagatcg
      361 gcgaagcctc ttgcggcgcc cgacacggcg gtcatcaggc tccacagcca cacgccgccc
      421 aggacaatgg cccgccgcgg ccaccggtcg gcggcgatgc cgatcgccat gccggcgacg
      481 gtgtagcaga ggacgaacgc gaagccggcc agcaggccaa tctgcgcccg cgagatctgc
      541 aggtccgcca tcagcagcgg cgcgaggctg cccacgagct gccggtccac gaagttcagg
      601 acgttgaacc cggccatcag ccagagcagc gcccaggggc ggcctgggac gaggccggcc
      661 ggcccggctg gcgggagggg cctggatgtc atcggctccc gatgataccc cgctcccgcg
      721 gcgctcggcg ttccggtata gactgcttgg tgtcgccctt atgggggccg tccgccagcc
      781 ctacgctgag gagccgccga tgtccgagtt ccagaagcac ctgtacctga ttctccaccc
      841 gaacgaagcg ctggtggcgt cgcagctctc cccggaggca ttcggcgccc actattcgat
      901 cggcagccct cgccatttca cgggaaaagt catcttcgcg gaagtagaca tcagcttccg
      961 gcacccggat ctgcgcatcg acgagtacct cgcgcagacg gagtcgggcc agtccggccg
     1021 gccgaagcgc acgaagttcg tcaagtccta ccgggtcctc gaacacatcg atctgaccgc
     1081 gttccagcgc ctgtacctcg tcacgacgga cggggcggtg ctgggtctcg acccgggcga
     1141 cgagccggcc gacacgcgcc gggcgggccg catccgcatc taccaggaga tctgcccgct
     1201 ccgcctggtg gtagcctcga gcctcgagcc gcgcgccttc ggcgcgtaca tcaccgccga
     1261 gacgtggtcg aaaggcgcgc cgaagatctt cttcgcccaa tgggacgtgg acgccgaggc
     1321 catcgtcaac cgcctcgacg tcctgtcgca cagcggccag ctcccgaacg tgaacccggc
     1381 gaacctctcg aacgcgcttc gggaactggc cgaaatcccc ggcaagaaga gcaagacgct
     1441 cagcctcaac ggcaacctgg acctgacgag cttcaagaac atccagccgg gcttctactt
     1501 ctcggagggt gacaagctgc tcttctaccg catgccgacg atggaagaac tgcacgacaa
     1561 gcaccgcgct tggtggcgtc acctatagac ggtttcgaga cctgcgtgct gcgcgggaac
     1621 gggtctcgaa accgcgttcc gtgagcgtca ccgctcctgc gcgctgcgcg ggaacgggtc
     1681 tcgaaaccgc gttccgtgag cgtcaccgcc cctgcatgct gcgcgggcac gggcctcgca
     1741 actgcgttcc gtgagcgtca ccgcccctgc gtgctgcgcg ggaccgggtc tcgcaaccgc
     1801 gttccgtgag cgccaccgct ccgccgcgcg cccccgggga cctgtctgtc gcgagactgt
     1861 cgatcggtgc cggtcactcg cgcagcgccg tcgccatccg ctccagcgcc tcggcgagcg
     1921 tcgcacgcgt gcacccgaag ttgaggcgca cgaatccttc gccgccgggc ccgaaccact
     1981 cgccgtcctg cagggccacg cgcgcgcgct cgaggaagaa ctgctgcggg ttcccgtcga
     2041 tgccgcacgt ccggcagtcc agccaggcca ggtaggtcgc ctcgggcggg gccatggctg
     2101 cccccggcag ctcgcggcgc acgaactccg cgacgaagtc ccggttcgcc gccagatagg
     2161 cgagcacctg atcgagccag tcctggccgt cccggtaagc ggcgagcgtc gccgccagcg
     2221 cgaggttgtt cacctccgaa aacgtggccg actcctcgcc gacgaggcgg cggcgcaggt
     2281 ccgggtcggt gacgacggcg aacgagcagc ggaggcccgg cacgttgaac gtcttgctcg
     2341 gcgccatcag cgtcacgcag cgcttcgcca cctcggggtc gagcgacgca atcggcgtgt
     2401 gcgcgctccc ggggaacacg agatcgcagt gaatctcgtc cgagcagatg aggacgtcgc
     2461 gcctcaggca catcgaggcg aggcgctcga gatcggcgcg gctgaacacg cggccgacgg
     2521 ggttgtgcgg gttgcagagg atgaagacgc gggtccggcc ggtgacggcc gcctcgaacg
     2581 cctcgaaatc gatctcccac cggccgtctg cgcgccgggc cagcggcgcc tgctgcccga
     2641 cggacccgtt gtgcgccgct gcgcccagga tcggctgata cacgggcgtc tgcacgagga
     2701 ttccgtcggt cgggccggcg gcggcacggc acgcgcggtg gaacgccgtc accacgcccg
     2761 tcccgagcac gatgtcgccg gccgacacgt gccagccgta gaggcggccg agccgctcgg
     2821 cgaggatctc gcggagctcg ggaggctcga cgcagtaccc gaaaacgccg tgatcgacgc
     2881 gcgcccgcag ggccgcgatg acgggctcgg gaacgcggaa gtccatgtcc gcggtccaga
     2941 gggggagcac gccgggcccg taaacgcgcc acttggtgct gtcggagccg atgcggtcga
     3001 cggggcggtc gaagtcgtag gtcatagggg cactatacct tcgggactcg ggacttggga
     3061 ttcgggagcc gggatccgtg gggcgcggac acggagcctc gcgggcacga gcaggggcgg
     3121 ccagcggcgg ctcgggtgct gagtcgtgca ttcagcgtcc agtcgtgcta tgaagcagcc
     3181 tgcaggaggc tgagaccatg ccatccgaga tgaaccggcg cgagttcctg tccggcgcgg
     3241 ccgcggccgc cgcctcgttc accatcgtgc cgaggcgcgt gctcggcggc gcggggttcg
     3301 tcgcgccgag cgacaagatc accctcgcgt gcatcggctt cggcacgcag gcgatccggg
     3361 agatcggcgg cattctcgcc agcccggacg tggaggtggt cgccgtctgc gatgtcgaca
     3421 agacgggcgt gggctacctc gagtggggca aggatcagat ccgcaacgac atcaggcgga
     3481 tcatcgacga gccgacgtgg cgcgagaacg tcaagggcgt acccggcggg ctggacgtgg
     3541 gcaaggagat cgtggacacc tactaccgca agcagcgcgg cgccgcgccc ggcgcgaagg
     3601 gctgcgcggc gtacgtggac taccgcgagc tcctcgagca ggagaaggac gtcaccgccg
     3661 tgaaggtcct gacgcccgac cacacccacg cgcccatcgc cattgccgcc ctcaggcgcg
     3721 gcatgaacgt gatcgtccac aagccgctgg ccaaccgcgt gctggaggcg cgcgccgtca
     3781 tcgaggccgc gcgggcggcg aacaaggcca cgcacttcct gccggccagc gagggcgcaa
     3841 gccagcggca ggcgctcgag atgatcggga acggcgcgat cgggaccctg cgggagatcc
     3901 acaactggtc cgcgcgcccg atgtggccgc agttcccgac gctgccggcc gacacgccgc
     3961 cggtgccggc ggggttcgac tggacgctct ggctcgggcc gtcgctcgac cggccgtacc
     4021 acccgaacta cacccacacg aacttccgcg gctggtacga gttcggcggc gggtccatcg
     4081 cggacatggg gcactacagc ctgtggccga tcttccaggc cctcgagctg gatgcgccac
     4141 tcagcgtcga gtcgacgccg agccacgtgt gcgccgtcgc cgacgacatc tgctacacga
     4201 tcaagaacga ctactccttc ccggcggcct gcacggtgcg gatgcgcttc ggcgcgaagc
     4261 gggccaggcc gggcctcgac atcttctggt acgacggcgg catcaggccg ccggtgccgg
     4321 aggaactgat ggcggagaac aaggagctgc cgagcgaggg catgctgttc gttggcgata
     4381 cgggcaagat cctcggcggc ttccgggccg agaacccgca gatcgtcccc gaggcgaaga
     4441 tgcgcgccta ccgggcggcg cacaacatcc cggagccggc gccgccgcag ccgcggcagc
     4501 gcggcccgca ggacctgcgg ccgcggccgt cggagcgcga tctcgcctgg attaacgcct
     4561 tcaagggcgg cccgcggagc tacggcgact tcctgctggc ccagccgatc tgcgacgcgg
     4621 tcaacctggc cgccgtctcg ctgcgcctcg gcggccggcg gctgctctgg gacgcggccg
     4681 cggcgagagt cacgaacgtg cccgaggcga acgcgctcct gacgcgcgag taccgcccgg
     4741 ggtggcagat ctagggagca ccagccatga caggactgac gagacgcgaa ttcatcgagc
     4801 gcacgggcgc ggcggccggg ttcctggcgg cggggatgcg cggcctggcg gcggccccgc
     4861 tgggcctgcc catcggcagc caggtctatc ccatgcggca cctgctgaag gacttcccgg
     4921 cgttcgtgaa ggcgatggcc ggcatcggcg tgacgcggct cgagctgtgc tcgcccatcg
     4981 ggtacggcac cgagttccag ccgctctcca acggcaggga ggtccgggcg atcctcgcgg
     5041 atcacgggct gaaagccgaa agcagccact tcagc
//
LOCUS       DAOSIF010000191         5053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14802_length_5053_cov_4.059824, whole genome shotgun sequence.
ACCESSION   DAOSIF010000191 DAOSIF010000000
VERSION     DAOSIF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14802_length_5053_cov_4.059824"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="PLT35_07565"
     CDS             complement(<1..1838)
                     /locus_tag="PLT35_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29121.1"
                     /translation="MLSQLAAVCRKEPTRTKWVLVPSRTLGHTLGERAARESGGWLNA
                     RFTTPEDLATATAAPHLVEAGINPLGEELGAPLVMRLLRELPPATPSYFRSLAEHHTM
                     GAALWSTIAELRLAGLGSAGLKAGAFESPAKHAELQALLTAYEDWLLRERRADEAAVF
                     RTALEHVAECPLREDDLILEALDTPWPPLVRRFIDALPGEHLARQATELPGVPLPRRL
                     SAFPRHEQRAASPLARLMAPPGPTATNLKAKPGKAQAAKANLAFFRAGGPDAEVEEVF
                     RRILHSPAGPRRLDEVEIACASSEYALLLWQKAERYGWPVTVETGLPGTVARPVRALL
                     AWCGWIDSGFEAGRLRRLLASGDVRMDIEGGPGSGQAARLLLRAAPAWGRQTYALALD
                     ALAVAETERAEDPELDDGERERHARRAADAQRLRDWIDGLTGRIPEAANGLVPVQALV
                     SAALDFVDRAAAVTNELDGQAARGIKARLEELRLLGDVDCTVLTALDTIRAALDTVRV
                     GSDRPRPGRLHVSSLARAGLAGRPVTFIVGLQEGEVFPAAFEDPVLLDVERVRTNEAA
                     AHVALALSADRTAEFVHGVAARLADLQGADAPGEPSICLSYSCQDLR"
     gene            2234..3058
                     /locus_tag="PLT35_07570"
     CDS             2234..3058
                     /locus_tag="PLT35_07570"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG29122.1"
                     /translation="MERDREAGGAGSVTIAGVELVLAPPDDHDVEWIDFDARIQRLAA
                     AWLRFSPKEPPLTSRIIGEPGVGKTTLACAVAHRLGRPPYIFQCTMDTRPEDLIVTPV
                     LTADRRVSYHASSLVSAMIRGGIAVLDEGNRMPERAWASLASLMDDRRYVESAAASVK
                     IHAHTDFRLCVTMNQDASVYELPGYIQSRLKPRVEVLNPPWELQEQILRQKCPTMDAA
                     LLASVFELLKQRVERGVHDTTRDMLALALYVEKLRRSDVDDPLGCAARQVLEKADA"
     gene            3051..5033
                     /locus_tag="PLT35_07575"
     CDS             3051..5033
                     /locus_tag="PLT35_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29123.1"
                     /translation="MRDGTGLTRRFRNLTAVPSRHHDPVFGRAVHEVFAAQVPDVVAL
                     ELPPSLSGELAWGASVWPEPAASIDMRSARRKKSKATILPFVPGDSILEGWRLARAHG
                     VEVALVDIDTRSGANGPDAGSIDVPGAELAGRVGRAFHEVADACLANSPPSAADLARE
                     AAMARALAVLMRHHGTVLWIGGMYHWARLVARLEAGDFRAPRVGRVARREWTRVALES
                     PAFYRLAGVLPWMSQAFAADPAGFTDAEATLRLLHAAAAEPPDLADGAPESHAPVDVA
                     RVDRYARNLAFSTGVRERAEADELLLAASAVIGSGYASRLYALAMKDPERAQTGDLEA
                     LTWEVDAEHQRAGFRLHGEWIALEPLRPARFPIAVLEPPDELLRHAHRAEYEGLPDAK
                     SGEKYFWGAYPPDEAEWESFVGYLLRRASIQDPGEARVFPFTAGLADGVDVRATIRFW
                     HQDHIYVREEQRGLLNVRNGAIDWSDEEDSDVPSGRAAGGWNDPDSVRVGSASRETEA
                     AEVLSQAGESLVTRRRREWSLMTLDLPTFRPAGGGGTRPGPDTFFGHVIEPLLSIKGE
                     PGQDHVYGWLEAMFKFCAGKPFAYYSRYRPSPRIHALAREHRVTLLWQPLGLVPAALL
                     KRNRTFRQLWLSASQWAALSSRLGLGAPSPGTPRRF"
BASE COUNT          683 a         1785 c         1851 g          734 t
ORIGIN      
        1 cgcaagtcct ggcaggagta gctgaggcag atggacggct cgccgggcgc gtccgcgccc
       61 tgcaggtccg ccaggcgggc cgccacgccg tgaacgaact ccgcggtgcg gtcggccgaa
      121 agggcaagcg ccacgtgcgc cgccgcttcg ttcgtgcgca cgcgctcgac gtccaggagg
      181 accgggtctt cgaacgccgc cggaaagacc tcgccctcct ggaggccgac gatgaaggtg
      241 accggccggc cagccagccc ggcgcgtgcc agcgaagaga cgtggaggcg gcccggccga
      301 ggccgatccg agccgacgcg caccgtgtcg agcgcggccc ggatcgtgtc gagcgccgtc
      361 aggaccgtgc agtccacgtc gccgaggagg cgcagttcct cgagccgggc cttgatgccg
      421 cgcgcagcct ggccgtccag ctcgttcgtc acggccgcgg cccggtccac gaagtcgagc
      481 gcggccgaga cgagcgcctg caccggcacc aggccgttcg cggcctcggg gatgcggccg
      541 gtcaatccgt cgatccagtc gcgcagccgc tgcgcgtcgg ccgctcgcct cgcgtgccgc
      601 tcccgctccc catcgtccag ttcgggatct tcggcccgct ccgtctcggc gaccgcgagg
      661 gcgtccaggg cgagcgcgta cgtctggcgt ccccaggctg gcgccgcgcg cagcagcagg
      721 cgtgccgcct gtccgctgcc cgggccgccc tcgatatcca tgcggacgtc gcccgacgcg
      781 agcaggcgcc gaaggcggcc ggcctcgaag ccgctgtcga tccagccgca ccaggccagc
      841 agcgcgcgaa ccggtcgcgc gaccgtgccc ggcaagcccg tttccaccgt gaccggccag
      901 ccgtagcgct cggccttctg ccagagcagc agcgcgtact cgctcgacgc gcacgcgatc
      961 tcgacctcgt cgaggcgccg cggcccggcg ggggagtgga ggatgcggcg gaacacctct
     1021 tcgacttccg cgtccggtcc tcccgcgcgg aagaaggcca ggttcgcctt cgcggcctgc
     1081 gccttcccgg gcttcgcctt caggttcgtc gccgtcggac cgggcggggc catcagccgc
     1141 gccagcggag acgcggcccg ctgctcgtgg cgcgggaacg ccgacagacg gcgggggagc
     1201 ggcactccgg ggagttccgt cgcctggcgc gcgaggtgtt cgccgggaag cgcgtcgatg
     1261 aaccggcgca cgagcggagg ccacggcgtg tccagcgcct cgagaatcag gtcgtcctcg
     1321 cggagcgggc actcggcgac gtgttcgagc gccgtccgga agacggctgc ctcgtctgcg
     1381 cggcgttccc ggaggagcca gtcctcgtag gcggtcagca gcgcctgcag ttccgcgtgc
     1441 ttcgccgggc tctcgaacgc gcccgccttc aggcctgccg agcccagtcc cgccagccgc
     1501 agttcggcga tggtggacca gagcgccgcg cccatcgtgt ggtgttcggc gaggcttcgg
     1561 aagtaggacg gcgtggcggg aggcagttcg cgcagcaggc gcatcacgag cggcgcgccg
     1621 agttcctcgc caagcgggtt gatgcccgcc tctactaggt gcggcgcggc cgtggcggtg
     1681 gcgaggtcct ccggcgtcgt gaaccgggcg ttgagccagc cgccagactc gcgggccgcc
     1741 cgttccccga gcgtgtgacc cagtgtccgg gagggcacga gcacccactt cgtgcgcgtc
     1801 ggctccttcc ggcagaccgc ggctagttgg ctgagcatgc gcgctcccac cccggcagcc
     1861 agtcctcgac gaactccgcg caggcgttgt ccgggtccgc cgcgcgttcg cgtatggcag
     1921 agactaccat cgcccgggac tcccggtgga cgtgttccgg atgcaggatg atgacgtcct
     1981 gcgcgctctc aaccgcgggt gattcgggcg ccatggtttc cactgcgcca gcatagagga
     2041 ccggtgtgac agggcgggtc gcaccgtgga gggtggtcct gatgcgacga gctcagaggc
     2101 actcgtaggc ccgcacgaac tcgtcggtgc aggtgttggc agtttctgcg ccccgcggcg
     2161 ggcttcgggc gagcagattc tgccgtgacc tgcgctgtcg cagcactccg gtagtctgtt
     2221 ggtgggaggt gcgatggaga gagacagaga ggccggcgga gcggggagcg tgaccattgc
     2281 cggcgtcgag ctggtgcttg cgccgcccga cgaccacgac gtggagtgga tcgacttcga
     2341 cgcgcgcatc cagcgcctgg cggcggcgtg gctgcggttc tcgccgaagg agccgccgct
     2401 cacgtcgcgc atcatcggcg agcccggcgt cggcaagacg acgctggcgt gcgccgtggc
     2461 gcaccggctg ggccggccgc cgtacatctt ccagtgcacg atggacacgc ggccggagga
     2521 cctgatcgtc acccccgtcc tgacggccga ccggcgcgtc tcctaccacg cgtcgagcct
     2581 cgtgtcggcc atgatccgcg gcggcatcgc cgtcctggac gagggcaacc ggatgcccga
     2641 gcgcgcgtgg gcctcgctgg cgtccctcat ggacgaccgg cggtacgtcg agagcgcggc
     2701 cgcctcggtg aagatccacg cgcacaccga cttccgcctg tgcgtcacca tgaaccagga
     2761 tgccagcgtc tacgagctgc ccggctacat ccagagccgg ctcaagccgc gcgtggaggt
     2821 gttgaacccg ccgtgggagc tgcaggagca gatcctgcgg cagaagtgcc cgacgatgga
     2881 cgccgcgctg ctcgcgtcgg tgttcgagct gctcaagcag cgcgtggagc gcggcgtgca
     2941 cgacacgacg cgcgacatgc tcgcgctggc gctctacgtc gagaagctga ggcgctcgga
     3001 cgtcgacgat ccgctcgggt gcgccgcgag gcaggtgctg gagaaggccg atgcgtgacg
     3061 gcaccggcct cacgcggcga ttccggaacc tgacggccgt gccgtcccgc caccacgacc
     3121 ccgtgttcgg ccgggccgtc cacgaggtgt tcgcggcgca ggtgccggac gtcgtggcgc
     3181 tggagctgcc gccatcgtta tccggggagc tggcgtgggg cgcgtcggtc tggcccgagc
     3241 ccgccgccag catcgacatg cgatccgcgc ggcgcaagaa gagcaaggcg accatcctgc
     3301 cgttcgtgcc tggcgactcg atcctagagg gctggcggct ggcccgggct catggcgtcg
     3361 aggtcgcgct cgtggacatc gacactcgat caggcgcgaa cggcccggat gccgggtcca
     3421 tcgatgtgcc gggcgccgaa ctggccgggc gcgttggacg cgcctttcac gaagtggccg
     3481 acgcctgctt ggcgaactcg ccgcccagcg ctgccgacct ggcgcgggaa gcggccatgg
     3541 ccagggctct ggccgtgctg atgcgccacc acggcacggt cctctggatt ggcggcatgt
     3601 atcactgggc acgcctcgtg gcgcggctcg aggccggcga cttccgcgcg ccgcgcgtcg
     3661 gccgcgtcgc caggcgcgag tggacgcgcg tcgccctcga gtcgccggcg ttctaccggc
     3721 tggccggcgt cctgccgtgg atgtcccagg cgtttgccgc cgaccccgcc gggttcacgg
     3781 atgccgaggc gactctgcgc ctgctgcacg ccgccgcagc ggagccgccc gacctggcgg
     3841 atggcgcgcc cgagtcgcat gcgcccgtgg acgtggcgag ggtcgatcgc tacgcccgca
     3901 acctggcgtt cagcaccggg gtccgcgagc gcgccgaggc cgacgagttg ctgcttgccg
     3961 cgagcgccgt gatcgggtcg ggctacgcgt cgcggctgta cgcgctggcg atgaaagacc
     4021 ccgaacgcgc gcagaccggc gatctcgagg cgctcacctg ggaagtcgat gctgagcacc
     4081 agcgcgccgg gttccgtctg catggcgagt ggatcgcgct cgagccgctt cggccggcgc
     4141 ggttcccgat cgccgtgctc gagccgcccg acgagctgct gcggcacgct caccgcgcgg
     4201 agtacgaagg cctgcccgac gcgaagagcg gcgagaagta cttctggggt gcctacccgc
     4261 cagacgaggc cgagtgggag tcgttcgtcg gctacctgct tcgccgggca agcatccagg
     4321 accctggcga ggcgcgtgtc ttcccgttca ccgccggcct cgccgatggc gtggacgtgc
     4381 gcgccaccat ccgcttctgg catcaggacc acatctacgt gcgcgaggag cagcggggcc
     4441 tgctgaacgt gcgcaacggc gccatcgact ggtccgacga ggaggacagc gacgttccgt
     4501 ccggccgggc cgcgggcgga tggaacgatc cagattcggt ccgtgtcggg agcgcgtcgc
     4561 gcgaaaccga ggctgcggag gtgctgtccc aggcagggga gtcgctcgtg acgaggcgcc
     4621 ggcgcgagtg gtccctgatg acgctcgacc tgccgacgtt cagacccgcc ggcgggggcg
     4681 ggaccaggcc cggacccgac acgttcttcg gccacgtgat cgagcccctg ctctccatca
     4741 agggcgagcc cggccaggac cacgtctatg gctggctcga ggccatgttc aagttctgcg
     4801 cgggcaagcc ctttgcttac tacagccgtt atcgcccgag cccgcgcatc catgcactgg
     4861 cccgggagca ccgggtgacc ctattgtggc agccgcttgg cctcgtgccg gcggcgctat
     4921 tgaagcggaa ccggacgttc cggcagctct ggctctcggc gagccagtgg gccgcgctca
     4981 gttcacgcct cggcctgggc gcgccgtccc cggggacgcc tcgccggttc tgatggagcc
     5041 tcgaggtttg cag
//
LOCUS       DAOSIF010000192         5052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14805_length_5052_cov_3.899540, whole genome shotgun sequence.
ACCESSION   DAOSIF010000192 DAOSIF010000000
VERSION     DAOSIF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14805_length_5052_cov_3.899540"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1048)
                     /locus_tag="PLT35_07580"
     CDS             complement(233..1048)
                     /locus_tag="PLT35_07580"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOG29124.1"
                     /translation="MTTLARGPRRPAAFALLAVALAWSAAHVSAQGAAPAAKPKVLMV
                     WGGWEGHEPKQTVGIFAPWLVEQGFDVEISDTLDSYLDTEKMKGLSLVVQVVTMGRIT
                     PQQEKGLLDAVRSGVGLAGWHGGLSDAFRNNTEYEFMVGGAWAAHPGGVIDYTVNVVS
                     HDDPITRGLKDFRMHSEQYYMLVDPNSEVLATTTFSGAHAPWIDGAVMPVAWKKLYGR
                     GRVFHLSVGHVAADFSVPEARTIMQRGLLWAARVPGFGEDPRPTNPYRLLRGK"
     gene            complement(1068..1853)
                     /locus_tag="PLT35_07585"
     CDS             complement(1068..1853)
                     /locus_tag="PLT35_07585"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOG29125.1"
                     /translation="MTRGIIVSAATAVSIAVAGLAVSAQLPPNPRAAELQQWAIHDLT
                     RPMPPVVDPGPAGPPAPVPSDATVLFDGRDLSAWTTAKGQPVTWPVRDGYMEVVKGAG
                     AIQTKAAFGDCQLHVEWASPAPAVGASQDRGNSGVFLMNTYEVQILDSYESATYADGM
                     AASIYGQFPPLVNASRRPGEWQSYDIVFRAPRFAADGTVTAPARMTVFHNGILVHDND
                     QLTGPTAHKARPPYKAHADRLPIGLQDHAHPVRFRNIWIRDLQ"
     gene            2150..2926
                     /locus_tag="PLT35_07590"
     CDS             2150..2926
                     /locus_tag="PLT35_07590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG29126.1"
                     /translation="MTPLLALVVPCYNEAERLQPGAFREFLASHDGVRLIFVDDGSRD
                     GTFALQERIRADAPGRVSILRMPSNRGKGEAVRLGILEGIRSGAGIVGFWDADLATPL
                     DAVDDFLAIAARRPDLEILIGSRVRLMGRDIRRQHGRHYLGRVFATAVSVSLDLPVYD
                     TQCGAKLFRVTGEMASVFERPFHSPWIFDVEALARYLALPVGPGERPRESRIYEVAVR
                     QWHHVPGSKLRWFDYIRSFFELGFIHRRYRRRPAPRGRGC"
     gene            3068..4531
                     /locus_tag="PLT35_07595"
     CDS             3068..4531
                     /locus_tag="PLT35_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29127.1"
                     /translation="MRFALKHAPEAGDRKMIDEQDTSNHVSRRSFVGGLAAAAGVLIV
                     PRRVLGGPGYQAPSDTVNVATVGYAHGMGTSNTNAVGKVANVVALCDCDESEAAQRAI
                     ARTKIWETFPKAVHYKDYRVMLEKQKDIDGVIVATPDHTHAVIAMAAMQLGKHVYVQK
                     PLTRTVSEARALTEAARRYKVVTQMGNQGHSGEGVRLVEEWIADGAIGKVHEVHCWTN
                     RPVWPQGMPRPAEGLPVPPGLHWDLFIGPSPMRPYHTAYHPFSWRAWWDFGAGALGDM
                     ACHVMDASYQSLKLGYPTSVTTYLAYNVMAVPREGGGTRNERIKYDDSFPPACVVHYT
                     FPARGKNLPALKLHWYDGGLQPERPEELEIDRRLPESGTIFVGEKGKLMCETYSESPR
                     LIPERRMQAYKRPKKTIPRVVGSHEQNWIEAIQGKTRATSNFDYSGPFTETVLLGNVA
                     AKFPGQKLLWDGRALRFTNSEEANAAIQHQYREGWSL"
     gene            complement(4630..>5052)
                     /locus_tag="PLT35_07600"
     CDS             complement(4630..>5052)
                     /locus_tag="PLT35_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29128.1"
                     /translation="ALGNATDFAITRLGLPIRNALAALGPKEFFCPGSTLRVAFDTTH
                     PLAYGMPPTGLVLFHYSPAFDIVATDVNDRHQIVARYADRDVERSGWLNGERHIAGRT
                     AMAASSYGQGQVVLIGFRTQNRAQTHGTYKLLFNALVK"
BASE COUNT          711 a         1794 c         1786 g          761 t
ORIGIN      
        1 gcctgacgcg tgacgtgctg ttcgaggcct acgtcgccta cgtcgtgcgc ggctgaagag
       61 gcccggggtc ccggagggcc gcactccgtc tgaaggcccc ggggctgcaa ccggcgcgcg
      121 cacacccggc cgcgcggccg cggcggcgcg cttcgagaag atccggcgtc cagccgggcc
      181 cggtgtgttc gccttcgcgg ccttccgtgt cctgcgggtg gcccaggcag gcttacttgc
      241 cgcgcagcaa gcgataggga ttggtgggcc tgggatcctc tccgaagccc ggaacgcggg
      301 ccgcccagag caagccccgc tgcatgatgg ttctcgcctc cggcacgctg aagtccgctg
      361 ccacgtggcc caccgacagg tggaacacgc ggccccttcc gtacagcttc ttccacgcca
      421 ccggcatgac cgcgccgtcg atccacggcg cgtgcgcgcc gctgaaggtc gtcgtcgcca
      481 ggacctccga gttcgggtcg acgagcatgt agtactgctc cgagtgcatc cggaagtcct
      541 tcaggcccct ggtgatcgga tcgtcgtggc tcacgacgtt caccgtgtaa tcgatcaccc
      601 cgcccgggtg ggccgcccac gccccgccca ccatgaactc gtactcggtg ttgttgcgga
      661 acgcgtcgct gagcccgccg tgccagccgg cgaggcccac gcccgacctg accgcgtcga
      721 ggaggccctt ctcctgctgg ggcgtgatcc ggcccatcgt gaccacctgc accaccagcg
      781 acaggccctt catcttctcg gtgtccaggt acgagtcgag cgtgtcggag atctccacgt
      841 cgaagccctg ctcgacgagc cagggcgcga agatgccgac cgtctgcttc ggctcgtggc
      901 cctcccagcc gccccacacc atcagcactt tcggcttcgc ggccggcgcc gcgccctggg
      961 ccgaaacgtg cgccgcggac caggcgagcg ccaccgccag cagcgcgaac gccgctggcc
     1021 gacggggccc gcgcgcgagt gtcgtcatgg cgcgtccctc cccgctgcta ctgcaggtcg
     1081 cggatccaga tgttgcggaa gcgcaccggg tgggcgtgat cctgcagccc gatcggcagg
     1141 cgatccgcgt gggccttgta gggcggccgg gccttgtggg ccgtcggccc ggtcagctgg
     1201 tcgttgtcgt gcaccaggat gccgttgtga aagaccgtca tccttgccgg cgcggtgacc
     1261 gtcccgtcgg cggcgaagcg cggcgcccgg aagacgatgt cgtaggactg ccactcgccg
     1321 ggcctgcggc tggcattgac gaggggaggg aactgcccgt agatcgacgc cgccatgccg
     1381 tccgcgtacg tcgcgctctc gtacgagtcg aggatctgca cctcgtacgt gttcatcagg
     1441 aagacgccgc tgttcccgcg atcctggctc gcgccaaccg ccggcgcagg cgaggcccac
     1501 tcgacgtgca actggcagtc gccgaaggcc gccttcgtct ggatcgcccc tgcgcccttg
     1561 acgacctcca tgtagccgtc acgcaccggc cacgtgaccg gctggccctt cgccgtcgtc
     1621 caggccgaca ggtcccggcc gtcgaagagc accgtcgcgt ccgacggcac cggcgccggc
     1681 gggcccgccg ggcccggatc cacgaccggc ggcatcggcc gcgtcaggtc gtgaatggcc
     1741 cactgctgca gctcggcggc gcgggggttc ggcggcaact gggccgacac ggcgaggccg
     1801 gccacggcga tgctcactgc ggtcgctgcg ctgacgatga ttccgcgtgt cacgtgtcct
     1861 ccgttcgcct cggagcgcgc cggcggcctc ccgccctggc cccttcgggg gcgggggcct
     1921 acggcgcgtc cggacgattc tacggcgaat cggcccgatg gcggatcctg gaccctctcc
     1981 tcaccaggcg gactgggggc ggcgcggcgg acctcggccg gcggggcctc ccggcgctct
     2041 ccgcctggcg cccggttccc gggtttcggc cccgggccgc cggtttccgg gagttgcccc
     2101 cttccctcca cctgtgtcac tgtgacgccg agacggacga cgcgcttcga tgacgcctct
     2161 gctcgccctg gtcgtgccct gctacaacga ggccgagcgc cttcagcccg gcgcgttccg
     2221 ggagttcctc gcgtcgcacg acggcgtccg cctcatcttc gtggacgatg ggagccgcga
     2281 cgggacgttc gcgctccagg agcgcattcg ggcggatgcg cccggccgcg tgtcgatcct
     2341 ccggatgccg tccaaccggg gcaaaggcga ggcggtccgg ctgggcatcc tggagggcat
     2401 ccggtcgggg gccgggatcg tgggcttctg ggacgccgac ctggcgaccc ctctcgatgc
     2461 cgtggacgat ttcctggcaa tcgccgccag gcggccggac cttgagatcc tgattggctc
     2521 gagggtgcgg ctcatgggcc gggacatccg ccgccagcac gggcgccact acctcggccg
     2581 cgtgtttgcg acggcggtct ccgtgagcct ggacctcccc gtgtacgaca cgcagtgcgg
     2641 cgccaagctg ttccgcgtca ctggcgagat ggcgtcggtg ttcgagcggc cttttcacag
     2701 cccgtggatc ttcgacgtcg aggcgctggc gcgctacctg gccttgcccg tcgggcctgg
     2761 cgaacggccg cgcgagtcgc ggatctacga ggtggcggtt cggcagtggc accacgtgcc
     2821 cgggtccaag ctgcgctggt tcgactacat ccggtcgttc ttcgagctgg gtttcatcca
     2881 tcggcgctac cgccgccggc ccgcgccgcg gggccggggc tgctgagcgg gggctgcctg
     2941 cggccgggcg ccgggggccc ggcgggacgc cggccggccg cgtcgtcgcg tctcccaggc
     3001 gccggcttcc cgtgaatttc ttcatccgtt ccggagccgc gaacggatag aatctggcga
     3061 tgatcgtgtg cgtttcgcgc tcaaacacgc ccccgaagcc ggagacagga agatgatcga
     3121 cgagcaggac acctcgaacc atgtttcacg gcgatcgttc gtcggaggac tggcggcggc
     3181 cgcgggcgtg ctgatcgtgc cgcggcgcgt gctgggcggg cccgggtacc aggcgccgag
     3241 cgacacggtg aacgtcgcga ccgtcgggta cgcgcacggc atgggcacca gcaacaccaa
     3301 cgccgtcggc aaggtggcga acgtcgtcgc gctctgcgac tgcgacgaga gcgaggcggc
     3361 gcagcgggcg atcgcccgga ccaagatctg ggagaccttc ccgaaggccg tgcactacaa
     3421 ggactaccgc gtcatgctcg agaagcagaa ggacatcgac ggcgtcatcg tggcgacgcc
     3481 cgatcacacg cacgcggtga tcgcgatggc cgccatgcag ctcggcaagc acgtctacgt
     3541 gcagaagccg ctgacgcgga ccgtgtcgga ggcccgcgcg ctgaccgagg ccgcgcgccg
     3601 ctacaaggtc gtgacgcaga tgggcaatca gggccactcc ggcgagggcg tgcgcctggt
     3661 cgaggagtgg atcgccgacg gcgccatcgg caaggtgcac gaggtccact gctggacgaa
     3721 ccggccggtc tggccgcagg gcatgccgcg cccggccgag ggcctgcccg tcccgccggg
     3781 cctccactgg gacctgttca tcgggccctc gcccatgcgg ccctaccaca ccgcgtacca
     3841 cccgttcagc tggcgcgcgt ggtgggactt tggcgccggc gccctcggcg acatggcgtg
     3901 ccacgtcatg gacgcctcgt accagtcgct gaagctgggc tacccgacga gcgtgaccac
     3961 ctacctcgcc tacaacgtga tggcggtccc gcgcgagggc ggcgggacgc gcaacgagcg
     4021 gatcaagtac gacgacagct tcccgcccgc ctgcgtcgtg cactacacgt tcccggcgcg
     4081 cggcaagaac ctcccggcgc tgaagctcca ctggtacgac ggcgggctcc agcccgagcg
     4141 gccggaagag ctcgagatcg atcgcaggct gcccgaaagc gggacgatct tcgtcggcga
     4201 gaagggcaag ctcatgtgcg agacgtactc ggagagcccg cgcctgattc ccgagcgccg
     4261 catgcaggcg tacaagcgcc cgaagaagac gatcccccgg gtcgtcggca gccatgagca
     4321 gaactggatc gaggcgatcc agggcaagac cagggcgacg tcgaacttcg actactccgg
     4381 ccccttcacc gagacggtgc tcctcggcaa cgtcgcggcg aagttccccg gccagaagct
     4441 gctgtgggac ggcagggcgc tgaggttcac gaactccgag gaggccaacg cggccatcca
     4501 gcaccagtac cgcgagggct ggagcctgta ggctcgtctc gggcggcgac accggaaggg
     4561 ccgggcccgg catcctgcga cgccgggccc ggcccctgcg ctcgttcctc ccgcggcgat
     4621 cctccgccgc tacttcacca gcgcgttgaa aaggagcttg tacgtgccgt gggtctgggc
     4681 gcggttctgc gtccggaacc cgatgagcac cacctggccc tggccgtagc tcgacgcggc
     4741 catcgcggtc cggccggcga tgtgccgctc gccgttcagc cagccgctcc gctcgacgtc
     4801 gcggtcggcg taccgcgcca cgatctggtg ccggtcgttg acgtcggtgg cgacgatgtc
     4861 gaaggcgggg ctgtagtgga agagcacgag gccggtcggc ggcatgccgt aggccagcgg
     4921 gtgcgtggtg tcgaacgcga cgcggagcgt cgagcccggg cagaagaact ccttcgggcc
     4981 gagcgcggcc agggcgttgc ggatcggcag gcccagcctg gtgatggcga agtccgtcgc
     5041 gttgccgagg gc
//
LOCUS       DAOSIF010000193         5009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14958_length_5009_cov_3.972346, whole genome shotgun sequence.
ACCESSION   DAOSIF010000193 DAOSIF010000000
VERSION     DAOSIF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14958_length_5009_cov_3.972346"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PLT35_07605"
     CDS             complement(<1..1226)
                     /locus_tag="PLT35_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG29129.1"
                     /translation="MDDKGLYFWRGPLPETQALPPDAAPEPWGETRVVGKPVTRVDAY
                     DRVSGSAIYPSDVILPDMLHAAVLTCPHAHALVKKVDTAGAEKMPGVRAVLKDGMPGT
                     NVPWFQGRGGFSSRLFDPHCRYEGDEVAVVAADTIYQAWDAVRAIDVEYEVLPHAVTV
                     EDAMKPDAPGLRDGGNVPPAPKPYERGNVAEGFKAADVVLEQTYTTPCELHNPMELHG
                     CVAKWDGPRLTVWESTQGVYAVQSGVANALKLPLANVRAIGPYMGGGFGSKLETGKYS
                     VMAALLARKTGRPVRLFYTREQVCRSMGNRPANTISLKVGAKRDGTLVALESRAVGTG
                     GAYAEDGTGGVDYLIRELYTCPNVRCENSSALTNAGPQRAFRAPGHPQGAWALESLMD
                     ALAARLQALGVRAADLA"
     gene            complement(1230..1868)
                     /locus_tag="PLT35_07610"
     CDS             complement(1230..1868)
                     /locus_tag="PLT35_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOG29130.1"
                     /translation="MSHDEQGKPAATATGVSRRGFITSVGSGAIGVAAAGALVKGAPP
                     SPPPDTLKAGDLSAVTITVNGRRHQVLVEARWSLLYVLRDRLGLTGTKIGCERGECGA
                     CTVLIDGKARYACMTLAVEADGHEVTTVEGLMQGEELGPVQQAFAEDDAFQCGYCTPG
                     QVMAAEGLLRENPSPTIEDVRHGMAGNICRCGAYAHIFTAVVKAADLKRGGR"
     gene            complement(2016..3164)
                     /locus_tag="PLT35_07615"
     CDS             complement(2016..3164)
                     /locus_tag="PLT35_07615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG29131.1"
                     /translation="MTHRMGTAVSLAVCAALVATAAFAQKKPAPPAAKLFTTPLTIDE
                     MTGKQAVIETTLGRIVIDLLPAQAPNHVGYFIKLAREGAYDGTTFHRLVARGIIQGGD
                     PLSKDPAKAAQYGTGGLRVLAFEPGAGTHVAGAVSSVLLPGNKNSAGAQFFICVVPQP
                     ALDGQYTMFGKVVEGIGIAQLISETPVDEAGKAATRVEMTSVTIRDALPPGVEPFSTE
                     PVEELASYRATLETSLGAITLELLPEKAPNTVRNFLRLASTGVYDRTSFHRVVPAFMI
                     QAGYLGTKQVPMDESQKALVRTLEPEFNDTPHEKGVVSMARGQDRASASTSFFIMTTR
                     VSSFDGIYTAFARVVDGMSVVEAIEQAPRDGEAPAPRIDLLRVRVSKK"
     gene            complement(3207..3563)
                     /locus_tag="PLT35_07620"
     CDS             complement(3207..3563)
                     /locus_tag="PLT35_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG29132.1"
                     /translation="MDATDLRSLVDGCRAGDALAWEGFVRRLQGRIFALAYSYVGERE
                     DARDLAQEIFVRLYETRAQWAEGDEFLPWLFRVARNRSVDYLRRRKVRAPGIAVDEPA
                     IAGLPDPEPGAEPAGE"
     gene            complement(3649..4611)
                     /locus_tag="PLT35_07625"
     CDS             complement(3649..4611)
                     /locus_tag="PLT35_07625"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOG29133.1"
                     /translation="MHISSGLHSAALIAVRDCMGLQPGERALVVTDAPLRTIGYAVFE
                     AARGLGNEAVLVEMPPRRTNGEEPPAEVAELMTRFDVVLCPTSKSLTHTDSRRQASAT
                     GARIATLPGVTEEVMVRCMNADYQAIAARTFRLCDLMRTTKVIRVTAPAGTDITMPVE
                     GREPHASSGLFREKGLWGNLPTGEAYLAPLEGLSTGVVVVDGSMASIGMVDEPIRIAV
                     QDGCATEIAGGESARRLVELLEPHGRDGRTVAEFGIGTNDKAILTGVILEDEKVMGTI
                     HIAFGDNKSMGGSVRVASHLDGLVKRPTVWFDDRLVMEDGRLLA"
BASE COUNT          683 a         1815 c         1733 g          778 t
ORIGIN      
        1 gccaggtcgg ccgcgcgcac gccgagcgcc tgcaggcgcg ccgccagggc gtccatcagg
       61 ctctcgagcg cccaggcgcc ctgcgggtgg cccggcgccc ggaaggcccg ctgcggcccc
      121 gcgttggtga gcgcgctgct gttctcgcag cgcacgttcg ggcaggtgta gagctcgcgg
      181 atgaggtagt cgactccccc ggtgccgtcc tcggcgtacg ccccgccggt gccgacggcc
      241 ctggactcca gggccaccag cgtgccgtcc ctcttcgccc ccaccttcag cgagatcgtg
      301 ttcgcggggc ggttgcccat gctcctgcag acctgctcgc gcgtatagaa cagccgaacc
      361 ggccgcccgg ttttgcgcgc cagcagggcg gccatgaccg agtacttccc ggtctcgagc
      421 ttgctgccga acccgccgcc catgtacggg ccgatggcgc gcacgttcgc caggggaagc
      481 ttcagcgcgt tcgccacgcc ggactgcacc gcgtacacgc cctgcgtgga ttcccagacc
      541 gtcaggcgcg ggccgtccca cttcgcgacg cacccgtgca gctccatcgg attgtgcagc
      601 tcgcacggcg tcgtgtaggt ctgttcgagc acgacgtcgg cggccttgaa cccctcggcg
      661 acgttgcctc gctcgtaggg cttcggggca ggcgggacgt tcccgccgtc gcgcagcccg
      721 ggcgcgtccg gcttcatcgc gtcctcgacg gtgacggcat gcggcagcac ctcgtactcg
      781 acgtcgatcg cgcgcacggc gtcccaggcc tggtagatcg tgtccgccgc caccacggcc
      841 acctcgtcgc cctcgtaccg gcagtgcggg tcgaacagcc ggctcgagaa cccgccgcgg
      901 ccctggaacc acggcacgtt cgtgcccggc atgccgtcct tcaggaccgc tcgcacgccc
      961 ggcatcttct cggcccccgc cgtgtccacc ttcttcacca gcgcgtgggc gtgcgggcag
     1021 gtcagcacgg cggcgtgcag catgtcgggc aggatcacgt cggacggata gatcgccgac
     1081 ccgctgacgc gatcgtaggc gtccacgcgc gtgacgggct tgccgacgac gcgcgtctcg
     1141 ccccagggct cgggcgccgc gtccggcggg agcgcctgcg tctcggggag cgggccgcgc
     1201 cagaagtaga gtcccttgtc gtccacggct tacctccccc cgcgcttcag gtcggccgcc
     1261 ttcacgacgg ccgtgaagat gtgcgcgtag gcgccgcacc ggcagatgtt gccggccatt
     1321 ccatgccgca cgtcctcgat ggtgggcgac gggttctcgc ggagcaggcc ctcagccgcc
     1381 atcacctggc cgggcgtgca gtagccgcac tggaacgcgt catcctcggc gaaagcctgc
     1441 tgcaccgggc ccaactcctc gccctgcatc aatccctcga ccgtcgtgac ctcgtggccg
     1501 tccgcctcca ccgccagcgt catgcaggcg tagcgcgcct tcccgtcgat gagcaccgtg
     1561 cacgcgccgc actcgccccg ctcgcatccg atcttcgtcc cggtcaggcc gagccggtcg
     1621 cgcagcacgt acagcagcga ccaacgggcc tcgaccagca cctggtgccg gcgtccgttg
     1681 accgtaatcg tcaccgcgct gaggtcgccc gccttcaggg tgtccggcgg cggggagggc
     1741 ggcgcgccct tcaccagcgc accggcagcc gccacgccaa tcgccccgga tcccaccgag
     1801 gtgatgaatc cgcggcggct cacgccggtc gccgtcgccg cgggttttcc ctgctcgtcg
     1861 tggctcatgc gtacctcccc gggttcgggg ggattatacc ctcgggactc ggccctcggg
     1921 actccagctc cggggctcgg gagacaggag agcacgcagt acagcgcgcc tccattccgg
     1981 atcttgaatt ccggctgctg actcttggct cctgactact tcttcgacac ccgcacccgc
     2041 agcaggtcga tgcggggcgc cggcgcttcg ccgtcccgag gcgcctgctc gatggcctcg
     2101 acgacgctca tgccgtcgac gaccctcgca aacgccgtgt agatgccgtc gaaggaggaa
     2161 acgcgggtag tcatgatgaa aaaggacgtc gaggcgctgg cgcggtcctg gccgcgggcc
     2221 atggagacga cgcccttctc gtgcggcgtg tcgttgaact cgggctcgag cgtgcggacg
     2281 agggccttct gcgactcgtc catcgggacc tgtttggtgc cgaggtagcc cgcctggatc
     2341 atgaacgccg gcaccacgcg gtggaacgag gtccggtcgt agacgcccgt cgaggccagg
     2401 cggaggaagt tgcggaccgt attgggcgcc ttctcgggaa gcaactcgag cgtgatggcg
     2461 ccgaggctgg tctcgagcgt cgcgcggtag ctggcaagct cctccaccgg ctcggtggaa
     2521 aacggctcca cgccgggcgg cagcgcgtcg cggatcgtca ccgacgtcat ctcgacgcgc
     2581 gtggccgcct tgccggcctc gtccacgggc gtttccgaga tcagctgggc gatgccgatg
     2641 ccctcgacca ccttgccgaa catcgtgtac tggccgtcca gcgccggctg cggcacgacg
     2701 cagatgaaga actgcgcgcc cgcgctgttc ttgttccccg gcagcaggac cgacgacacc
     2761 gccccggcca cgtgagttcc cgctccaggc tcgaatgcca ggacgcggag gccgccggtt
     2821 ccgtactggg cggccttcgc ggggtctttc gacagcgggt cgccgccctg gatgatgccg
     2881 cgggcgacga gccggtggaa ggtcgtgccg tcgtaggccc cttccctggc gagcttgatg
     2941 aagtagccga cgtggttggg cgcctgcgcc gggagcagat cgatgacgat gcggccgagc
     3001 gtcgtctcga tgacggcctg cttgcccgtc atctcgtcga tcgtgagggg cgtggtgaag
     3061 agcttcgccg ccggcggggc cggcttcttc tgggcgaagg cggcggtcgc gacgagggcg
     3121 gcgcagaccg cgagcgagac ggcagtcccc atccggtgcg tcatggctgg cctccggatg
     3181 gggattatag cggcttgtcc gccgttctac tcgccggccg gctcggcgcc cggctccggg
     3241 tccggcaggc ccgcgatggc gggctcgtcc accgcaatcc ccggcgcgcg caccttccgc
     3301 cggcgcaggt agtccaccga gcggttgcgt gcgacgcgga acagccacgg caggaactcg
     3361 tccccctccg cccactgggc gcgggtctcg tacagccgga cgaagatctc ctgcgccagg
     3421 tcgcgcgcgt cctcgcgctc gccgacgtag ctgtaggcaa gggcgaagat gcggccctgg
     3481 agccggcgga cgaagccttc ccaggcgagc gcgtcgcccg cgcggcagcc gtcaacgagg
     3541 gagcgaaggt cggtggcgtc cacggatcgg ggctgacggc actcgggcgc gcctcgccat
     3601 tcgggataac gcgccgggcg cccggaaggt tccgcgcccc cctgtcggtc acgccagcag
     3661 gcggccgtcc tccatgacga gccggtcgtc gaaccacacc gtcggcctct tgacgaggcc
     3721 gtcgaggtgg ctggcaacgc ggacggaacc gcccatcgac ttgttgtcgc cgaaggcgat
     3781 gtgaatcgtc cccatcacct tctcgtcttc gaggatgacg ccggtgagga tggccttgtc
     3841 gttcgtgccg atgccgaact cggcgacggt gcggccgtcc cgcccgtgcg gctcgagcag
     3901 ttcgacgagc cgccgcgcgg actcgccgcc cgcgatctcc gtcgcgcagc cgtcctgcac
     3961 ggcgatccgg atgggctcgt cgaccatgcc gattgacgcc atcgagccgt ccaccacgac
     4021 gacgccggtc gacaggccct cgaggggcgc cagataggcc tcgcccgtcg gcaggttccc
     4081 ccagagcccc ttctcgcgga acagcccgga actcgcgtgg ggctcgcggc cctcgaccgg
     4141 catcgtgatg tcggtgccgg ccggcgcggt cacccggatg accttcgtcg tgcgcatcag
     4201 gtcgcagagg cggaacgtgc gcgcggcgat ggcctggtag tccgcgttca tgcagcggac
     4261 catgacctcc tcggtcacgc ccgggagcgt ggcgatgcga gcgcccgtcg cgctggcctg
     4321 gcgccgcgag tcggtgtggg tcagcgactt cgaggtcggg cacaagacga cgtcgaacct
     4381 ggtcatcagc tccgccacct cggcgggcgg ctcttccccg ttcgtccggc ggggcggcat
     4441 ctccacgagc acggcctcgt tgccgaggcc ccgcgcggct tcgaacaccg cgtacccgat
     4501 cgtgcgcagc ggcgcgtcgg tgacgacgag cgcgcgctcg cccggctgaa ggcccatgca
     4561 gtcccggacg gcgataagcg cggcggagtg gaggccggag gagatgtgca ttggggcatt
     4621 gtagcggggg ctggcactcg cgtgtcatct ttacgggcgg aagggcctcc ccagggagca
     4681 atccgcccga tgccccgccg gacatcccca tacgatctgc tcgcggccgg cgcgataacg
     4741 agccccgtgt ccgtgcgaga tgcccctgtc gcgcctcgag atcccccttc ctggtgcagg
     4801 acacggcgcg caccgttcgc gccttccatc gcagtccggc cccatcggag ctgaggctgt
     4861 gcccctggtg ccgacgccca tggaccagca gcctggtcgt ccgcaacgca ctggaagggg
     4921 tgcgaccatg agagcactcc tggatgtcgt gctggcggcg ttcagcgatc gagcgaccgc
     4981 agcgagacgt ggcagagcat cgccggggc
//
LOCUS       DAOSIF010000194         5006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14969_length_5006_cov_4.071097, whole genome shotgun sequence.
ACCESSION   DAOSIF010000194 DAOSIF010000000
VERSION     DAOSIF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14969_length_5006_cov_4.071097"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLT35_07630"
     CDS             complement(<1..374)
                     /locus_tag="PLT35_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG29134.1"
                     /translation="MLYDVLVSLARPLGWWGRMRVDGLEHLPAEGPVLLAPNHDSQMD
                     PVLISLAALPRRMLRYLAGDDLWRIPLVGAVLDGMGQIPITRGRGDRRALERAVDALK
                     AGDAVTVFPEGRLSWGQRLRARL"
     gene            528..1442
                     /locus_tag="PLT35_07635"
     CDS             528..1442
                     /locus_tag="PLT35_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG29135.1"
                     /translation="MSIPEPPAIVTRGLMKRFGRLVAVRALDLQVEPGQVFGFLGQNG
                     AGKTTTIRMLLDLLRPTAGRAEILGHDCQADGLAARALVGYMPGEIGFYRDMTGRRAL
                     GVLAELARGAVRADRQRELCERMALDDADLDRPIREYSTGMKRKLAAVAAFQHDPPVL
                     LLDEPTEGLDPLMQEAFYELLADARAGGTTVFLSSHVLSEVDRVCDRIGLLRAGELVL
                     TAPIGEVRRTAPRLVRVTFRRPVVGPPSAWPPSIEIVEVAAARWIVRAYGSIGPVVES
                     LGGLPVADLEVREPRLEDVVMAYYRGQQ"
     gene            1463..2254
                     /locus_tag="PLT35_07640"
     CDS             1463..2254
                     /locus_tag="PLT35_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29136.1"
                     /translation="MRHSARRFRAPVAIAAAALGAFQVLLCLAARELQREGMFQQILG
                     LIPPFARELFGPVLVSMMSFPGIMALGYYHVAIVAVLVGLVIAIATEPAGEVESGFAD
                     LVLARAVPRGAVMTRSIVLLTGGAACVVAAMAAGTFFGLWLVGPADASKPASSLVWKL
                     AFSLWCVLACWGGVSLAIGAAARRRAVAASMAGSAAAALMLVDYLARFWKPARGAARL
                     SPFHYYNPLDLVIGKPLPLGDIAVLLGTGTVTAALAYLVFARRDL"
     gene            complement(2251..4725)
                     /locus_tag="PLT35_07645"
     CDS             complement(2251..4725)
                     /locus_tag="PLT35_07645"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOG29137.1"
                     /translation="MTSPAFTGRLPRAAAFAIVLAGAHGLTPPTHGPADAAGALSERV
                     MIRRDVFGIPHIVAETEEAAGFGLGYAQAEDHAAEMAKRFLAARGDAARHFGASFLEN
                     DLAARRVDNLGESRRALATLDRTFRGVLEGFAAGFNLYVRQHRDALPPWVGEVTAADV
                     LALTHADYPADVASPDLVRALQRKYPEEAARPAPGPIEGREIAGPRAAAGAGGTPERS
                     GGDIGSNALALSGRRTASGAAILLGNPHLRWESLYWEAHVKVPGRLDFYGSTLVGFPW
                     LRAGFNDRLGYVQTNNTSDNKDVFALRLDPERRDHYLFEGRPRPLARVDVAVDVRRDD
                     GTRATERRTYWQSHLGPIVYRTTTAAFALRSTGADAWKWFEGFWRLSHARSLREYLLV
                     MSTRFSPESNYTYADADGNIFYLWNSRIPKRVQDGTDYSLDVPGATKRYAWGSLHPTK
                     DLPQLLNPEGGYIQNANNPPWFVSMRPQLDPARYPAYFERGELALRPQLALDLIEARE
                     TFSVDDVVRLKYEPRLLVAERVVPALLTALDARLTGRENGAPGAGRTPAAPGTSDAGD
                     AGELVQARAVLRAWDRRVSASSRGAVLFQRFWETYRAEVRQPYAEPWDAARPTDTPRG
                     IADPDAALKHLAEAVRWTRAAYGAAEVAWGDVNRYRFGGVDLPGEGASGLLGAFRVQQ
                     FDEVPGASDAAGASDAARGRRVRVAGWAAADRQLAGSGDAWVLLVHFTRPIQAYSVLA
                     YGQTTDRASPHSRDQIGIFAKRGLRPIWFTDAEVSAHLERAYRPSDTERGRDELRNGT
                     SAKDDFVVTESSCEQAPLRNSSRPLH"
BASE COUNT          667 a         1776 c         1803 g          760 t
ORIGIN      
        1 aggcgcgcgc gcaggcgctg cccccaggac agccggccct cgggaaacac cgtgaccgca
       61 tcgcccgcct tcagcgcgtc caccgcccgc tccagggcac gcctgtcgcc gcggccgcgc
      121 gtgatcggaa tctggcccat gccgtcgagc acggccccga cgagtggaat cctccacagg
      181 tcgtcgccag ccagatagcg cagcatgcgg cgtggcagtg ccgcgagcga gatcagcacc
      241 gggtccatct ggctgtcgtg gttgggcgcg agcagcacgg gcccctcggc tggcaggtgc
      301 tcgaggccgt ccacgcgcat ccggccccac cagcccaaag gacgcgccag gctcaccagg
      361 acgtcgtaga gcatcgcacc cgccaacata aggggtcggg actcttttcg caacggcacg
      421 ccaggcaacg acggttttgt cgtcatcgac ggtggcacag accggcgctg acaaaaccgt
      481 cgctcgacag agcggcgttg cgaaaagagt cccgacccct tagactcgtg agcattcctg
      541 aacctcccgc catcgtcacc cgtggcctca tgaagcggtt cggccgcctc gtggctgtgc
      601 gggcgctcga cctccaggtt gagcccggac aggtgttcgg cttcctcggc cagaacggcg
      661 ccggcaagac cacgacgatt cgaatgctcc tcgacttgct gcggccgacc gccggccggg
      721 cggagatcct cggccacgac tgccaagccg atggtctggc agcgcgcgcg cttgtcggct
      781 acatgccggg agagatcggc ttctaccgtg acatgaccgg tcggcgcgcg ctcggggtgc
      841 tggcagagct cgctcgaggc gcggtgcgcg cagaccggca gcgcgagctg tgcgagcgga
      901 tggcgctcga cgacgccgat ctcgatcgcc cgatccgcga atacagcacc ggcatgaagc
      961 ggaaactcgc cgccgtcgcg gcattccagc acgacccgcc cgtcctgctc ttggacgagc
     1021 cgaccgaagg cctcgacccg ctcatgcagg aggcgttcta cgagctgctg gctgacgcac
     1081 gggcgggcgg gacgacggtg ttcctgtcgt cgcatgtcct gtcggaagtg gaccgcgtct
     1141 gcgaccggat tgggctgctt cgcgccggcg agctggtgtt gacggcgccg atcggcgaag
     1201 tccgccgcac ggcgccgcgt ctggtgcgcg tcacgttccg ccgcccggtc gtgggtccgc
     1261 cgtccgcctg gccgccgtcg atcgagatcg tggaagttgc cgccgcgcgc tggatcgtgc
     1321 gggcgtacgg gagcatcggg cccgtcgtcg agtcgctggg cggcctgccc gtggccgatc
     1381 tcgaggtgcg cgagccgcgc ctcgaggacg tcgtgatggc gtattaccgg gggcagcagt
     1441 gacgcgggag accgccgccc tcgtgcggca ctcagcccgc cggttcaggg cgccagtggc
     1501 gattgcggcg gccgcgctcg gcgcgttcca ggtgctgctg tgcctggcgg cacgagagct
     1561 gcagcgggaa ggcatgttcc agcagatcct cgggctcatc ccgcctttcg cccgcgagct
     1621 cttcggaccg gtgctcgtgt cgatgatgtc attcccgggc atcatggccc tcggctacta
     1681 ccacgtggcc attgtcgccg tgctcgtcgg cctggtcatc gccatcgcca ccgagcctgc
     1741 cggagaggtc gagagcggat tcgccgacct ggtgctggcg cgggcggtgc cgcgcggcgc
     1801 cgtgatgacg cgaagcatcg tgctcctcac gggcggcgcc gcgtgtgtcg tggcggcgat
     1861 ggccgcggga acctttttcg gtctctggct ggttgggccg gcggacgcat cgaagccagc
     1921 ctcgtcgctc gtctggaagc tcgcgttcag cctgtggtgc gtgctcgcgt gctggggcgg
     1981 cgtatcgctg gcgattggcg ccgcggccag gcggcgggcc gtcgcagcct cgatggccgg
     2041 atcggcggcg gcggccctga tgctcgtgga ctatctcgcg cgcttctgga agcccgcgcg
     2101 cggcgccgcg agactgtcgc cgttccacta ctacaacccg ctcgatcttg tcattggcaa
     2161 gccgctgccc ctcggggaca tcgccgtcct gctcggtacc gggacggtta ccgccgccct
     2221 cgcgtacctc gtcttcgccc ggcgggacct ctagtgaagg ggtcgggacg aattacgcaa
     2281 cggcgcctgt tcgcacgacg attctgttac gacaaaatcg tctttcgcgg acgtgccgtt
     2341 gcgtaattcg tcccgacccc tttctgtgtc ggaggggcgg tacgcgcgct ccaggtgggc
     2401 cgagacctcg gcgtcggtga accagatcgg ccgcagtccc cgcttcgcga agatcccgat
     2461 ctgatcgcgg ctgtgcgggg acgcgcggtc agtcgtctgg ccgtaggcca gcaccgagta
     2521 cgcctggatc ggccgcgtga agtgaaccag caacacccag gcgtcgcccg atccggcgag
     2581 ctgccgatcg gctgcggccc acccggcgac gcggacccgc ctcccgcgtg cggcgtctga
     2641 cgcgccggcc gcgtcggacg cgcctggcac ctcatcgaac tgctgcaccc ggaacgcgcc
     2701 gagcaggccg ctcgcgcctt cgcccggcag gtcgacgccg ccgaaccggt accggttcac
     2761 gtcgccccat gcgacctcgg cggccccgta cgccgcccgc gtccagcgga cggcctcggc
     2821 gaggtgcttc agcgccgcat cgggatcggc aatcccgcgg ggggtgtcag tcggccttgc
     2881 agcgtcccat ggctcggcat acggctgccg cacttcggcc cggtaggttt cccagaagcg
     2941 ctggaacagc accgcgcccc ggctcgacgc cgacactctt cgatcccacg ccctcaacac
     3001 ggcccgagcc tggaccaact cgcccgcgtc tcccgcgtcg gacgtgccgg gcgccgccgg
     3061 cgtccgtccc gcgccgggcg ccccgttctc gcggcctgtc aggcgcgcgt cgagcgccgt
     3121 gagaagcgcc ggcacgacgc gctcggccac cagcagccgc ggctcgtact tcagccggac
     3181 gacgtcgtcc accgagaacg tctcgcgcgc ctcgatcagg tcgagcgcca gttgcggccg
     3241 cagcgccagc tcgcccctct cgaagtacgc cgggtagcgc gccggatcca gctgcgggcg
     3301 catcgagacg aaccacggcg ggttgttggc gttctggatg tacccgccct cggggttcag
     3361 gagctgcggc aggtccttcg tgggatggag cgagccccac gcgtaccgct tcgtcgcgcc
     3421 cggcacgtcg agggagtagt ccgtgccgtc ctgcacccgc ttcgggatgc gcgagttcca
     3481 gagatagaag atgttcccgt ccgcgtcggc gtacgtgtag ttcgactcgg gcgagaaccg
     3541 cgtggacatc acgagcaggt actcgcgcag cgaccgggcg tggctcagcc gccagaagcc
     3601 ttcgaaccac ttccaggcgt cggcgcccgt cgagcggagc gcgaacgccg ccgtcgtggt
     3661 ccggtagacg atcgggccga ggtgcgactg ccagtaggtc cgccgctcgg tggccctggt
     3721 gccatcgtcc cgcctgacgt ccacggcgac gtcgacgcgc gccagcggac gcggccttcc
     3781 ctcgaagagg tagtggtcgc gccgttccgg gtcgagcctc agcgcgaaga cgtctttgtt
     3841 gtccgacgtg ttgttcgtct ggacgtagcc gagacgatcg ttgaatccgg cgcggagcca
     3901 gggaaacccg acgagcgtcg acccgtagaa gtccagccgc cccggcacct tcacgtgcgc
     3961 ttcccagtag agcgactccc agcgcaggtg cgggttgccg agcaggatcg cggcgccgct
     4021 cgcggtccga cggcccgaga gcgcgagggc gttcgacccg atgtcgccgc ccgagcgctc
     4081 gggtgtgccg cctgccccgg ctgccgcccg cggtcccgcg atctcgcgcc cctcgatcgg
     4141 gcccggcgcc ggtcgggcgg cctcttcggg gtacttgcgc tggagcgccc ggacgaggtc
     4201 gggtgacgcg acatccgccg ggtaatcggc gtgcgtcagg gccagcacgt ccgcggccgt
     4261 gacttcgccg acccatggcg ggagcgcgtc gcgatgctgc cgcacgtaca ggttgaaccc
     4321 agcggcgaag ccctcgagca cgccgcggaa cgtccggtcc agcgtggcca gcgcgcgccg
     4381 gctctcgccg aggttgtcca cgcgcctggc ggcgaggtcg ttttccagga acgacgcgcc
     4441 gaagtgcctc gcggcgtctc cgcgcgcggc gaggaagcgc ttcgccatct cggccgcgtg
     4501 gtcttccgcc tgcgcgtatc cgagcccgaa cccggccgcc tcctccgttt cggcgacgat
     4561 gtgcgggatg ccaaagacat cgcggcggat catcacccgc tcggacaggg ctccggctgc
     4621 gtcggcgggg ccgtgcgtcg gcggcgtcag gccgtgcgcg cccgccagga cgatggcgaa
     4681 ggccgccgcc cgggggaggc ggcccgtgaa agccgggctg gtcatgggcg gggatgatag
     4741 cgcggatcgg ccgagggttc tgcgattgca gcatccttct ccgcccggtt gtccgagcgt
     4801 tccggtttcg ggccggcggg ggccgggctg gctgtcggcg gcggcgcgtg gcagggcggc
     4861 cgcgcggcgc aggggggagc cgtagcccag ggggagtcgc ggcccaaggc ggcggcgcgg
     4921 ggcagggcgg ccgcgcggga cagggcagcg acgcgtggca gggcggccgc gcggcgcagg
     4981 ggggagccgt agcccagggg gagtcg
//
LOCUS       DAOSIF010000195         5002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_14981_length_5002_cov_4.758844, whole genome shotgun sequence.
ACCESSION   DAOSIF010000195 DAOSIF010000000
VERSION     DAOSIF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14981_length_5002_cov_4.758844"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1534
                     /gene="glmM"
                     /locus_tag="PLT35_07650"
     CDS             173..1534
                     /gene="glmM"
                     /locus_tag="PLT35_07650"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG29138.1"
                     /translation="MRAIPTLKISISGVRGVVGDSLTPTLLTRFAQAFGTYTGSGTVV
                     VGRDTRTSGEMVRQAVAAGLLSSGCRVVDLGVCPVPTVQLAVRHRRAHGGIAITASHN
                     PPEWNALKFVGPDGLFLGSGPARELLDIYHQGEYVKVPGANMRSIDVMTDGLDLHLKA
                     IMDGVGPLPAAPKRLRVALDGCNGAGALIAPRLLEALGADVVPINITPNGLFPRGAEP
                     VPENLGALCEAVRAGGCDIGFAQDMDADRLAVVSELGEPIGEDFTLVLAAWHVLATRP
                     GPVVANLSTTAALDEVARRFNCPVVRTKIGEANVTERMVSERAVIGGEGNGGVIYPAV
                     NFARDSQVGMALILHLLASSGKTVSALMAELPGFVMVKEKLACPSNRIPDVLRMVKRE
                     YAGLPMDTRDGVKVLLENGWFHVRGSNTEPIVRVVAEGPSRGAARAIVDEVFGRVAHV
                     VAS"
     gene            complement(1585..2406)
                     /locus_tag="PLT35_07655"
     CDS             complement(1585..2406)
                     /locus_tag="PLT35_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOG29139.1"
                     /translation="MTLRVAAAVALAGVGVALFAARLVAPGPADFYIEPETGMVLVAV
                     RPGSFMMGTNDAEPGRRDDEYLHRVTITQLFYIGQHEVTQAEWARVMGSNPSRFTGCE
                     KCPVEQVDFYQVNAFLSRINAGNTAMRFRLPTEAEWEYACRAGSRTAFSGGAQLPALA
                     ANVDTRFMAGPEDGAAYEKTLPAGRFPANKWGVHDMHGNVWEWTNDWYGPYDPRREFD
                     PRGADSGTRKAIRGGSWRFDAMSARCSARQAHPPQDAADDLGFRVVAEPRRPRRR"
     gene            2514..4481
                     /locus_tag="PLT35_07660"
     CDS             2514..4481
                     /locus_tag="PLT35_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29140.1"
                     /translation="MRTWRAAVLFAVLTLLLTWPLARHPSSATWSSGPDGDLFIWTLA
                     WDAHAFVHQPLSIFDANIYHPLRRTLAYSENLIGSALFAAPVIWLTGSPVLALNVAAL
                     LSCALCGLGAFVLARRVGLGAAGAVIAGLVFAFAPPRFFRTSQLHVGAVQWIPFALAF
                     LHAYLDKGRARDLRLAAACFSLQALASGHGAAFLFVAMTALAVYRAVRGTAIDAARRL
                     RDLGVTGALLLAPAVLVYLPYRAVQQETGLRRSLENWAVTPVSFLASPTHAHAWLASL
                     APGLRILEDASAYLFPGVLPLLLAASAIVLRPGGARRAMGGGHGPAGARRALAVLVRA
                     AAYFLEAAVAASLVLAVAVTLTGPLRLRAGGTVVVSARSALRPWLLAAAAALARAALL
                     GRVPFDPASRWRRSAAGVRRLGLRLRTPEPSGSVFFYGLLMLLGLWLSIGPPLGLWPL
                     VYWLPGMSFIRVPSRFTVLAVLGLAIVAGAGMDRLLARVSSRRRTLLPAAVAVLLVAE
                     SAAAPLSLVPAHAEAPLADRWLAARPRPFVVAEVPLPDPMDASAFERRQTMYMLHAMA
                     HWQKTVHGYSGWRATLHERLYLAMRAFPDDASLDALSRLGVTYVVVHTELYPPGEWPL
                     VEERLERFGARLQLEHVAGAGRVYALRPARP"
     gene            complement(4497..>5002)
                     /locus_tag="PLT35_07665"
     CDS             complement(4497..>5002)
                     /locus_tag="PLT35_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29141.1"
                     /translation="GVSARATFRNADLFETDLSEPTVITMFLLPQINMRLRPKILDLK
                     PGTRIVSNSFNMEDWIPDETATVSEGCETWCTAMLWIVPARIEGQWKTAEGDLSVVQT
                     FQQFTGTLQSGSESTPITEGRLSGASLTMTIGAARYEGRVNGHAIEGTVTEGEKTRPW
                     RASRLGS"
BASE COUNT          584 a         1886 c         1728 g          804 t
ORIGIN      
        1 gacggggggc taggcgcggg agtgcagggg ctgggggctc gccgctgggc gctgatccgc
       61 ccgtatgtct cgctggcgcg ccgcccgcgc ggcgacagcc gtgttccact agtctctgcc
      121 gcccagtccc cacctcctga ttcgtagtcc agagcgagct gaggcgtttg tgatgcgagc
      181 gatacccact ctgaagatca gcatctccgg cgtgcgcggc gtcgtgggcg actcgctgac
      241 gcccacgctg ctgacgcgct tcgcgcaggc gttcggcacg tacacgggat ccggcacggt
      301 cgtcgtcggc cgcgacacgc gcacgtcggg cgagatggtg cgacaggccg tcgcggccgg
      361 cctcctgtcg agcggctgcc gggtcgtcga tctcggcgtg tgcccggtgc cgacggtgca
      421 gctcgccgtc cgacaccgcc gcgcgcacgg cgggattgcc atcacggcca gccacaatcc
      481 gccggaatgg aacgcgctga agttcgtcgg cccggacggg ttgttcctgg gctccggccc
      541 ggcccgcgag ctgctcgaca tctaccacca gggcgagtac gtgaaggtgc ccggcgcgaa
      601 catgcggtcg atcgacgtca tgaccgacgg cctcgatctc cacctgaagg ccatcatgga
      661 cggagtcggg ccgctgccgg ccgcgccgaa gcgcctgcgc gtcgcgctcg acgggtgcaa
      721 cggcgccggc gccctcatcg cgccgcgcct gctcgaggcg ctcggggccg acgtcgtgcc
      781 catcaacatc acgccgaacg gcctgtttcc gcgcggcgcc gagccggtcc ccgagaacct
      841 cggcgcgctc tgcgaggccg ttcgcgccgg cggctgcgac atcggcttcg cgcaggacat
      901 ggacgccgac cgcctcgccg tcgtctcgga gctcggcgag cccatcggcg aggacttcac
      961 cctcgtcctc gccgcgtggc acgtgctggc gacccgcccc ggcccggtgg tggccaacct
     1021 ctcgacgacg gccgccctgg acgaggtggc gcgccgcttc aactgccccg tcgtgcgcac
     1081 gaagatcggc gaggccaacg tcaccgagcg catggtgtcc gagcgcgcgg tgattggcgg
     1141 cgagggaaac ggaggcgtca tctacccggc cgttaatttc gcccgcgaca gccaggtggg
     1201 catggcgctc atcctgcacc tgctcgcctc gtccggcaag acggtgtcgg ccctcatggc
     1261 cgagctgccg ggcttcgtga tggtgaaaga gaagctcgcc tgcccgtcca acaggatccc
     1321 ggacgtgctg cgcatggtga agcgggagta cgccggcctg ccgatggaca cgcgcgacgg
     1381 cgtgaaggtc ctgctcgaga acggatggtt ccacgtccgc ggatcgaaca ccgagcccat
     1441 cgtgcgggtg gtggccgaag ggccgtcgcg cggcgcggcg cgcgcgatcg tggacgaggt
     1501 gttcggccgc gtggcgcacg tcgtcgcctc ctgagcgatc gccgcggggc cagatccgca
     1561 tattttcaga tctgacccca acggtcagcg cctgcgcggg cggcgcggct cggcgacgac
     1621 gcggaacccg aggtcgtcgg cggcgtcctg cggcgggtgg gcctggcgcg cgctgcagcg
     1681 cgcgctcatc gcatcgaacc gccagctgcc gccgcggatg gccttgcgag ttccgctgtc
     1741 cgccccccgg gggtcgaact cccgcctggg gtcgtacggc ccgtaccagt cgttcgtcca
     1801 ctcccacacg ttgccgtgca tgtcgtggac gccccacttg ttcgccggga accttccggc
     1861 cggcagcgtc ttctcatacg ccgccccgtc ctcgggcccg gccatgaacc gcgtatcgac
     1921 attcgccgcc agcgccggca gctgcgcgcc gccgctgaag gcggtgcggc tgccggcccg
     1981 gcacgcgtac tcccattcgg cttccgtcgg cagcctgaac cgcatcgccg tgttcccggc
     2041 gttgatgcgg gagaggaagg cgttcacctg gtagaagtcc acctgctcga cggggcactt
     2101 ctcgcacccg gtgaagcggc tcggattcga ccccatgacg cgcgcccact ccgcctgcgt
     2161 cacctcgtgc tggccgatgt agaagagctg cgtgatcgtg accctgtgca ggtactcatc
     2221 gtcccggcgg ccgggctcgg cgtcgttcgt gcccatcatg aacgacccgg gccgcacggc
     2281 cacgagcacc atccccgttt cgggctcgat gtagaagtcg gccgggcccg gcgcgacgag
     2341 gcgcgcggca aagagcgcca cgcccacccc ggccagcgcg acggccgcag caacgcggag
     2401 ggtcaccccc agattctacc gccccgcccg ggcgggccgg ccctgaaacc cgcgcgtccg
     2461 ccgcgccggc cgaatccttc gcctttccgg tccccgcccc gtaaaatccg ggaatgcgca
     2521 cgtggcgcgc cgcggtcctc ttcgccgtcc tgacgctcct gttgacctgg cccctcgccc
     2581 gccatccctc gtcggcgacg tggtcgtcgg gccccgacgg ggacctcttc atctggaccc
     2641 ttgcgtggga cgcgcacgcg ttcgtccatc agcccctgtc gatcttcgac gcgaacatct
     2701 accacccgct ccgccgcacg ctggcgtact ccgagaacct gatcgggagc gcgctctttg
     2761 cggcaccggt catctggctg accggcagcc ccgtcctggc gctgaacgtc gccgcgctcc
     2821 tgtcgtgcgc gctgtgcggc ctcggcgcct tcgtgctggc gcgccgggtt ggactcgggg
     2881 ccgccggggc cgtcatcgcc ggcctcgtct tcgcgttcgc gccgccgcgc ttcttccgga
     2941 ccagccagct gcacgtcggc gccgtgcaat ggatcccgtt cgccctcgcc ttcctgcacg
     3001 cgtacctgga caagggccgc gcgcgcgatc tgcgcctggc ggctgcgtgc ttcagcctgc
     3061 aggccctcgc gagcggccac ggggcggcgt ttctcttcgt cgcgatgacc gcgctcgccg
     3121 tctaccgcgc cgtgcgcggg accgccatcg acgcggccag gcgcctccgc gacctgggtg
     3181 tgacgggcgc actcctgctc gcgccggccg tgctcgtcta tctgccctac cgggccgtgc
     3241 agcaggaaac cgggctgcgc cggtcgctcg agaactgggc cgtcacgcca gtcagcttcc
     3301 ttgcgtcgcc gacgcacgcg cacgcgtggc tcgcctcgct cgcgccgggc ttgcgcatcc
     3361 tcgaggacgc cagcgcgtac ctcttcccgg gcgtcctgcc tctcctgctg gccgcatcag
     3421 cgatcgtcct gcgcccgggc ggcgcgcggc gcgcgatggg cggtgggcac gggccggccg
     3481 gcgcacgccg ggccctcgcg gtcctcgtgc gcgcggccgc gtactttctc gaggcggctg
     3541 tcgcggcctc gctcgtcctg gccgtcgccg tgacgctcac ggggccgctg cggctccgcg
     3601 ccggcggcac ggtggtcgtg tccgcgcgca gcgcgctgcg gccgtggctg ctcgccgccg
     3661 ccgccgccct cgcgcgtgcg gcattgctcg gccgcgtgcc gttcgacccg gcctcgcgat
     3721 ggcggcgcag cgcggctggc gtgcgcaggc tcgggctgcg gctccggaca cccgagccgt
     3781 caggctcagt cttcttctac ggcctcctga tgctcctcgg gctctggctg tcgatcggcc
     3841 cgccgctcgg cctctggccg ctcgtgtact ggctgcccgg catgagcttc attcgcgtgc
     3901 cttcacggtt caccgtgctg gccgtgctcg gcctggccat cgtcgcgggc gcgggcatgg
     3961 accggctgct cgcgcgcgtg tcatcccggc gccggacgct cctccccgcc gcggtcgcgg
     4021 tcctgctcgt ggccgagtcg gccgccgcgc ccctgtcgct cgtgccggcc cacgccgagg
     4081 cgccgctggc cgatcgctgg ctcgccgccc ggccccgccc ctttgtcgtc gcggaggtgc
     4141 cgcttccgga tccgatggat gccagcgcgt tcgaacgccg ccagacgatg tacatgctgc
     4201 acgcgatggc gcactggcag aagaccgtgc acgggtacag cggctggcgg gcgacgctgc
     4261 acgagcggct ctacctcgcg atgcgcgcct tccccgacga cgccagcctg gacgcgctct
     4321 cccggctcgg ggtgacctac gtcgtggtgc acacggagct ctacccgccc ggggagtggc
     4381 ccctcgtcga ggagcggctg gagcgattcg gcgcgaggct ccaactggag cacgtggcgg
     4441 gcgccggccg cgtgtacgcg cttcgtccgg cccgtccgtg aagggccgcc gcgcggctac
     4501 gatccgaggc gcgaggcccg ccagggccgg gtcttctcgc cctcggtgac cgttccctcg
     4561 atcgcgtgcc cgttgacgcg gccctcgtac cgggccgcgc cgatcgtcat cgtcagactg
     4621 gcgccgctca gccggccttc ggtgattggc gtgctctccg atccggactg gagcgtgccc
     4681 gtgaactgtt ggaacgtctg aacgacgctc aggtcgccct cggccgtctt ccactggccc
     4741 tcgatcctgg ccggcacgat ccagagcatg gccgtgcacc acgtctcgca gccctcgctc
     4801 accgtggccg tctcgtcagg aatccagtcc tccatgttga acgagttcga cacgatgcgc
     4861 gtgccgggct tcaggtcgag gatcttcggc cgcagccgca tgttgatctg cggcagcagg
     4921 aacatggtga tgaccgtcgg ctcagagaga tccgtctcga acaggtcggc gttgcggaac
     4981 gtcgccctgg cggagacgcc gg
//
LOCUS       DAOSIF010000196         4997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15007_length_4997_cov_4.136180, whole genome shotgun sequence.
ACCESSION   DAOSIF010000196 DAOSIF010000000
VERSION     DAOSIF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15007_length_4997_cov_4.136180"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLT35_07670"
     CDS             complement(<1..897)
                     /locus_tag="PLT35_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG29142.1"
                     /translation="MLLKGATLVRIDPPALERADLRVAGDTIDGRGPRLRPRAGEAVV
                     DVKGRLILPGLVCAHTHGYSALSRGMPAPSASPRSFADMLARVWWTLDQALDEETVYY
                     SALVAAVEAVRAGTTTLIDHHSSPNAIPGSLDIVREALGAVGVRGVLCYEVTDRGGAR
                     RRDAGLAENDRFLSATRSDPQMRGLVGAHASFTLGDSSLAALGDLVRYHGAGIHMHVA
                     EATDDVRRTERAHGCGILDRLDRHGLLNERAVLVHGVHLSDADLARARGASAWLVHNP
                     RSNMNNAVGRARIERFGARAALG"
     gene            complement(902..2152)
                     /gene="thrC"
                     /locus_tag="PLT35_07675"
     CDS             complement(902..2152)
                     /gene="thrC"
                     /locus_tag="PLT35_07675"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG29143.1"
                     /translation="MATRMFTGLVCITCGRRVAPGDHQTCVACGSEGILDARYDYDGA
                     RAALTRDALGLRPRTIWRYEELLPVPPGAARPPLHTGWTPIVEAPRLAAALGIRRAWL
                     KDEGRNPTASLKDRASAIGVAKALEFGYRAIACASTGNAASSLAAMAASMGLPAFIFV
                     PERAPEPKIAQLLVFGATVFRVGGTYEQAFDLCRDACARFHWYNRNSGTNPFLVEGKK
                     TAGLEIAEQCAADPSFDGAMPDWVAVSVGDGCTIGGIAKGLHEFKRIGLAGAVPRMLG
                     VQAAGAGPIVEAFNARAGLVPCAPSTLADSIAVGTPRNWRRALAWVRATKGAMVAVPD
                     ADILDSLRDAARLGGVFGEPAGVACLAGLRAAVAAGTISRGATALAVITGNGLKDVRS
                     AMDAAGQPHDIRPDIEAVAAVVGG"
     gene            complement(2178..2969)
                     /locus_tag="PLT35_07680"
     CDS             complement(2178..2969)
                     /locus_tag="PLT35_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29144.1"
                     /translation="MRFADRTLGRTGLTVGPLGIAASYGAPAAAVERAVEAGLNYLYW
                     GSLRTKAFAEALRHLAPSRDRLVLVVQSYSRSAALLTWSVERALRRIGYDHADVLLLG
                     FWNGPVSPRIMEAARRLQRRGLVNALAVSGHKRAALAGFAAGGGVDVLHVRYSASHPG
                     AARDLFPHLPGGSGPGVVSFTALDYGRMVHPRYTPAGFRTPSAGDCYRFVLSHPAVDV
                     CMTGPRSAADVEQIIAARELGPMSGDELAWMTRVGEAVAARRRRN"
     gene            3030..4343
                     /gene="ispG"
                     /locus_tag="PLT35_07685"
                     /gene_synonym="gcpE"
     CDS             3030..4343
                     /gene="ispG"
                     /locus_tag="PLT35_07685"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOG29145.1"
                     /translation="MILHILGTAKGVMAMRAPGARHETIGVAVGGVRVGGGAPIVVQS
                     MCNTDTADVEATLRQCLELWQAGSEMLRVTVNVPEAAEAVPALKRRLAEAGCAAPLIG
                     DFHYNGHLLLTKYPACAKALDKYRINPGNVGTGMRRDEQFSTICRVAAGLGKPVRIGV
                     NAGSLNQELVTRRMQENTDRGLGLTSEQIIGECMVISAIESTALALQSGLREDQIIIS
                     CKASKPRSLIAVYRELARRTRQPLHLGLTEAGMGVKGLVWSASAMGVLLHEGIGDTIR
                     VSLTPPPGGDRREEVHAACELLQALGLRAFAPSVTACPGCGRTTSTTFQELAEQIQRH
                     VRERMPEWKARYDGVETMTLAVMGCVVNGPGESKAANIGISLPGTGEAPACPVYIDGR
                     HAATLRGTYDELSKAFRTLVDEYVEAKYPRRQGQGATDNGLGARR"
     gene            complement(4428..4562)
                     /locus_tag="PLT35_07690"
     CDS             complement(4428..4562)
                     /locus_tag="PLT35_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29146.1"
                     /translation="MTYGKGKLLILTPEVTFRAQPHAAFKFLFNAIYYGSARRVFLKN
                     "
     gene            complement(4586..>4997)
                     /locus_tag="PLT35_07695"
     CDS             complement(4586..>4997)
                     /locus_tag="PLT35_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29147.1"
                     /translation="ALPPQYQGRQGRVTAATMPALKEFLEQGGTIITIGSSASLAGHL
                     GLPVSNGLVEMVNGVERRLPAEKLYVPGSILTMDVDPSSPLAYGFGPAVDVMWDNSPA
                     LRLGPDAALRGSRRSAGSAATSRFAAAGRGARST"
BASE COUNT          730 a         1800 c         1829 g          638 t
ORIGIN      
        1 gccgagcgcg gcccgcgccc cgaaacgctc gatgcgcgcg cggcccacgg cgttgttcat
       61 gttggaacgc ggattgtgca cgagccacgc cgaggcgccg cgggcgcggg ccaggtccgc
      121 gtccgacagg tgcacgccgt gcaccagcac ggcgcgctcg ttcagcagcc cgtgccggtc
      181 cagcctgtcc aggatgccgc agccgtgcgc gcgctcggtg cgccgcacgt cgtctgtcgc
      241 ctccgcgacg tgcatgtgga tgccggcgcc gtggtaccgc acgaggtcgc ccagggcggc
      301 gagactggag tcccccagcg tgaaggacgc gtgcgcgccg accaggcccc tcatctgcgg
      361 atcggatcgc gtcgccgaca agaaccggtc gttctcggcc agccccgcgt cgcggcgccg
      421 cgcgccgccc cgatccgtca cctcgtagca cagcacgccg cgcacgccga ccgcgccgag
      481 cgcctcgcgg acgatgtcga gcgagccggg aatggcgttg ggcgacgagt ggtggtcgat
      541 gagcgtcgtc gtgccggcgc ggaccgcctc cacggcggcc accagggccg agtagtacac
      601 cgtctcttcg tccagcgcct ggtcgagcgt ccaccagacc cgcgccagca tgtcggcaaa
      661 cgaccgcggg ctggcggacg gggcgggcat gccccgcgac agggccgagt agccgtgcgt
      721 gtgcgcgcac accagcccgg gcagaatcag gcggcccttg acgtccacga cggcctcgcc
      781 ggctctcggg cgcagccgcg gcccgcggcc gtcgatggtg tcgcccgcga cgcgcaggtc
      841 cgcgcgctcg agagcgggag ggtcgatgcg gaccagcgtg gcgcccttca ggagcatcgg
      901 gtcaccctcc gacgacggcg gcgacggcct cgatgtccgg gcggatgtcg tgcggctgcc
      961 cggcggcatc catcgcggag cggacgtcct tcagcccgtt gcccgtgatg acggccagcg
     1021 ccgtcgcccc gcgggagatc gtccccgccg ccacggcggc gcgcagaccg gccaggcagg
     1081 cgaccccggc cggttcgccg aacaccccgc ccaatcgcgc cgcatcgcgc aaggagtcca
     1141 ggatgtccgc gtccggcacc gccaccatgg cgcccttcgt cgcccgaacc caggcgaggg
     1201 cgcggcgcca gttgcgcggc gtgccgacgg cgatgctgtc ggcgagcgtc gagggcgcgc
     1261 atggcacgag gccggcacgg gcgttgaagg cctcgacgat cgggccggcg cccgccgcct
     1321 gcacgcccag catccggggg acggcgccgg cgagcccgat ccgcttgaac tcgtggaggc
     1381 ccttggcgat gccgccgatc gtgcagccgt cgccgaccga caccgccacc cagtccggca
     1441 tcgcgccgtc gaacgagggg tccgccgcgc attgctccgc gatctcgagg ccggcggtct
     1501 tcttgccctc caccaggaac gggttggtgc cgctgttccg gttgtaccag tggaaccgcg
     1561 cgcaggcgtc gcggcagagg tcgaaggcct gctcgtaggt gccgccgacg cggaacacgg
     1621 tggcgccgaa cacgaggagc tgcgcgatct tcggctccgg cgcgcgctcc ggcacgaaga
     1681 tgaacgccgg gaggcccatc gacgccgcca tggcggccag cgacgaggcg gcgttgcccg
     1741 tcgaggcgca ggcgatggcg cggtagccga actcgagcgc tttggcgacg ccgatcgcgc
     1801 tcgcgcggtc cttcagcgac gccgtggggt tgcggccctc gtccttcagc cacgcgcggc
     1861 ggatgccgag cgcggcggcg aggcgcggcg cttccacgat gggcgtccac ccggtgtgca
     1921 gcggggggcg cgcggcgccc ggcggaaccg gcagcagctc ctcgtagcgc cagatcgtcc
     1981 tcggccgcag cccgagcgcg tcgcgcgtca gggccgccct ggcgccgtcg tagtcgtacc
     2041 tggcgtcgag gatgccctcg ctcccgcagg cgacgcacgt ctggtggtcg ccgggagcga
     2101 cgcggcggcc gcacgtgatg cagacgagtc cggtgaacat gcgcgtggcc aacagtagcg
     2161 cctgccgcgg cgcgcggtca atttcgccgg cggcgcgcgg caaccgcctc gccgacccgc
     2221 gtcatccacg ccagttcgtc cccgctcatc ggcccgagct ctcgcgccgc gatgatctgt
     2281 tcgacgtcgg cggccgagcg cgggccggtc atgcagacgt ccaccgccgg atgggagagg
     2341 acgaagcggt agcaatcgcc cgccgagggc gtcctgaagc ccgcaggcgt gtaacgcggg
     2401 tgcaccatcc ggccgtaatc gagggccgtg aacgacacga cgccgggccc ggatccgccg
     2461 ggcaggtgcg gaaagaggtc gcgcgcggcg cccggatgcg acgcgctgta gcgcacgtgg
     2521 agcacgtcga cgccgccgcc tgccgcgaag ccggcgagcg cggcccgttt gtgtccggag
     2581 acggccaggg cgttgacgag gccgcgccgc tggagccttc gggcggcctc catgatgcgc
     2641 ggacttaccg ggccgttcca gaagccaagc agcagcacgt cggcgtgatc gtagccgatg
     2701 cgccggagcg cccgctcgac gctccaggtg agcagcgccg cgctgcgcga gtacgactgc
     2761 acgacgagca caaggcggtc ccgggacggc gcgagatggc gcagcgcctc ggcgaacgcc
     2821 ttcgtgcgca ggctgcccca gtacaggtag ttcagtcccg cctcaacggc gcgttccacg
     2881 gccgccgccg gcgcgccgta cgatgccgca atgccgaggg gccccacggt gaggccggtc
     2941 ctgccgagcg tgcgatctgc gaaccgcatg gcgcctccag gccttccgcg gcgcgtggcc
     3001 catcggcagg ccgccgcgga gcgaatgata tgatccttca catcctgggg acggcgaaag
     3061 gcgtcatggc catgagggca ccgggtgcgc gtcacgagac catcggcgtg gctgtcggcg
     3121 gcgttcgcgt gggcggcggc gcgcccatcg tggtccagtc gatgtgcaac accgacaccg
     3181 ccgatgtcga ggcgacgctg cggcagtgcc tcgaattgtg gcaggccggc tcggagatgc
     3241 tgcgcgtgac ggtcaacgtc cccgaggccg ccgaggccgt gccggccttg aagcggcgcc
     3301 tcgccgaggc gggatgcgcg gcgcccctca tcggcgactt ccactacaac ggccatctgc
     3361 tgctgacgaa gtacccggcc tgcgcgaagg ccctcgacaa gtaccgaatc aatcccggca
     3421 acgtcgggac gggcatgcgc cgcgacgagc agttctcgac gatctgccgg gtggccgccg
     3481 gcctcggcaa gcccgtgcgc atcggcgtca acgccgggtc gctcaaccag gagctcgtca
     3541 cccggaggat gcaggagaac accgaccgcg gcctgggcct cacctccgag cagatcatcg
     3601 gcgagtgcat ggtgatctcg gcgatcgaat caaccgccct tgcgctccag agcgggctcc
     3661 gcgaggacca gatcatcatc tcctgcaagg cgtcgaagcc ccggagcctg atcgccgtct
     3721 accgcgagct ggcccgccgg acgagacagc cgctgcacct cggattgacc gaggccggca
     3781 tgggcgtgaa ggggctggtg tggtcagcct cggccatggg cgtgctcctg cacgaaggca
     3841 tcggcgatac catccgggtc tcgctgacgc cgccgccggg cggggaccgg cgcgaggaag
     3901 tgcacgccgc ctgcgaactg ctgcaggccc tcgggctcag ggcgttcgcg ccctccgtga
     3961 ccgcatgccc gggctgcgga cgcacgacga gtacgacctt ccaggagctg gccgagcaga
     4021 tccagcgcca cgtccgcgag cggatgccgg agtggaaggc ccgctacgac ggcgtcgaga
     4081 caatgaccct ggccgtgatg ggatgcgtgg tgaacgggcc gggggagtcg aaggccgcca
     4141 acatcggcat cagcctgccc ggcaccgggg aggcgccggc gtgcccggtg tacatcgacg
     4201 ggcggcacgc cgcgacgctg cgagggacgt acgacgagct gtcgaaggcg ttccgcacgc
     4261 tcgtcgacga gtacgtggag gcgaagtacc cgcgcaggca ggggcagggg gcaacggaca
     4321 acgggctggg cgccaggcgc tagtcagcct caccgcgacg ctgcgcggcg actgacgcgg
     4381 cgacagccgc gtccgaccag cccctggcgc caagtccccg gcgcctgtca gttcttcaag
     4441 aacaccctgc gcgcactgcc gtagtagatc gcgttgaaca ggaacttgaa ggcggcgtgc
     4501 ggctgcgcgc ggaacgtgac ctccggcgtc agaatcagga gcttcccctt gccgtaggtc
     4561 acgtcgagcg ccgtcgtcag ccccttcagg tgctcctggc cccacgccca gccgctgcga
     4621 agcggcttgt cgcggccgaa ccagccgacc ggcgtgatcc ccggagcgcc gcgtccgggc
     4681 ccagcctcag ggcggggctg ttgtcccaca tgacgtcgac tgccgggccg aagccgtacg
     4741 ccagcgggct tgacggatcg acgtccatgg tcagaatcga gccggggacg tagagcttct
     4801 cggccgggag ccggcgctcg acgccgttca ccatctcgac gagcccgttc gacaccggca
     4861 ggccgaggtg cccggcgagg ctggcggagg acccgatggt gatgatggtc ccgccctgct
     4921 ccaggaactc cttgagcgcg ggcatcgtcg ccgccgtcac gcggccctga cggccctggt
     4981 actggggcgg gagggcc
//
LOCUS       DAOSIF010000197         4995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15014_length_4995_cov_3.583198, whole genome shotgun sequence.
ACCESSION   DAOSIF010000197 DAOSIF010000000
VERSION     DAOSIF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15014_length_4995_cov_3.583198"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLT35_07700"
     CDS             complement(<1..687)
                     /locus_tag="PLT35_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29148.1"
                     /translation="MPGLGTRGRLLGVLFAALGSAAGPGALSPPSASQDAMPAASRVL
                     ARFAREDNPIALRGPARPGRYLEASGRKAALIGREDGSFEAWVYPLKVLHDFSLSFRT
                     PDYAEPIPGASVASAVDVRPEASTVRYAHAAFTADATWLVPLSETGALVLLDVSTSQP
                     LQVVVKFRADLKPMWPAALGGQYSYWDPATKAYVLGEASGRHAAMIGSPLGLDPPEQP
                     AHNLPDAPSQF"
     gene            1212..2171
                     /locus_tag="PLT35_07705"
     CDS             1212..2171
                     /locus_tag="PLT35_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOG29149.1"
                     /translation="MAAPPAIRIGVSSCLLGQAVRFDGGHKRNPLLVETFGRFVEWIP
                     VCPEVELGLGVPRETLRLTRTADGEVRMVTTKSGRDITAQMREWSRARTEALAGEGLS
                     GYVLKKDSPSCGMERVTVSGGSGAALRTGRGLFAEALMARFPRLPIEDEGRLSDPRLR
                     DNFVERAFAYRRLGALFAGRWQPDDLIRFHAAHELALLAHSPAIEAELARLAARGTSM
                     ARADLEIRYRDRFMDAMATLATTRKHTNVLRRMARRLAPLLGADAWQELQSNLEEYRL
                     GLAPLVVPLALVRRQVRRHRVEQLAGQVYLDPHPTELMLRNHV"
     gene            2271..3968
                     /locus_tag="PLT35_07710"
     CDS             2271..3968
                     /locus_tag="PLT35_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG29150.1"
                     /translation="MRGRRLLQVILLAAALLPYFVNLGATSIIDANEAFYAQTPVEMM
                     ASGDCISPTFNGEPRLNKPPLSYWAVAASYKVFGVSLFAARLPIALGAVVIVMTAFFL
                     GQLAFSPGAGFVAAVTLAASPRVLLFSRRIIIDIYTTMFVGLALACFARAEIEPQPAR
                     RRAWLLAMYAAIGLGVMTKGPVAIALPGLVFAGYLALIGRLRTVTRLMLPTGAVVVAA
                     IVAPYYAALCAQEGWGAISSFLLRENLARYTSGVGAPDRGPLFYLPVVLGDFYFPWSL
                     LLPAAFALVPWRSVLAGRGWARIWGGPGAEGPVAPERVRLLLGLWIILIVAFFSLSNG
                     QQDLYVLPFVTAAAPLVGGLVAAWAGSRITGRLDRVATWSVAAIAVALAAAGAGVAWF
                     VGRPGERIHLAGALATGGVLIIGGAAALAALKRHARAVAAIGLAAAAILSLWIIVIRS
                     LPDYERYQPVPQLARVIRAGTGPAPAVATYLVAAPSLVFYLNRHVHQLFDREQLHAFL
                     RANPSGLCLMTDDEYAAVRESLPVPTHVVASAPRMGSKLVDFLDRAPLRMLVLIGPA"
     gene            complement(4280..>4995)
                     /locus_tag="PLT35_07715"
     CDS             complement(4280..>4995)
                     /locus_tag="PLT35_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG29151.1"
                     /translation="PPWDLSVPDNVRVDVWLEEFRRFEKDGGLPALSILRIGGDHTAG
                     TRAGYPTPRAMIAENDAALGRIVEAVSRSVYWKESAIFVLEDDAQNGPDHVDAHRSPA
                     FVISPFVKRGVVDSTMYSTSGMLRTMELILGLPPMSNYDAAATPMYNAFQATPVLTPY
                     RARPARIDVTEKNAVNAWGAAASAAMNMAEADLCPDLELNDIVWRSVRGANHPMPPPV
                     RAAFVRALPGDDDDEEDRD"
BASE COUNT          659 a         1794 c         1769 g          773 t
ORIGIN      
        1 gaactgcgag ggggcgtccg gcaggttgtg cgccggctgc tccggcgggt ccaggccgag
       61 gggggatccg atcatcgccg cgtgcctgcc gctcgcctcg ccgagcacgt acgccttcgt
      121 ggccgggtcc cagtagctgt actggccgcc gagggccgcc ggccacatcg gcttgaggtc
      181 cgctctgaac ttcaccacga cctgcagcgg ctgcgacgtg ctcacgtcga gcagcaccag
      241 cgcgccggtc tccgacaggg gcaccagcca cgtcgcgtcc gcggtgaagg cggcgtgggc
      301 gtagcgcacg gtgctcgctt cgggccgcac gtccaccgcc gacgccacgc tcgcgcccgg
      361 gatcggctcg gcatagtccg gcgtcctgaa cgagaggctg aagtcgtgga gcaccttgag
      421 cggatagacc cacgcctcga agctgccgtc ctcgcgtccg atgagcgccg ccttgcggcc
      481 cgacgcctcg aggtaacggc cgggccgcgc cgggccgcgg agggcaatcg gattgtcctc
      541 gcgcgcgaac cgcgcgagaa cgcggctggc ggcaggcatc gcgtcctgcg acgcagacgg
      601 cggcgaaagc gcccccgggc cggccgccga gccgagcgcc gcgaacagga ctccgagcag
      661 gcggccgcgc gtgccgaggc cgggcatgac atccctccgc cgcctgcagc cgcggtccgg
      721 cgccggcggc ccgacgcggc ggattgtagc aaatcgccgt cggcgctcga tgtcgatgag
      781 ctggccgggc ccatcacagg catcgtgaag cgccctcgct ccggacccgg cggcgcccat
      841 caccggcgct gccggccgca ttcgacctgg ggctcaccgc cgcctgtctc cgcgccgccc
      901 ggcgttgaca ccgcggcgag atccaggccg acggctagct cccgcccgag cgcaggctgc
      961 gccggttcgt cgccggccgc ctcaccgtgc gcgcccgcgg gcggaagccg ccccgaacgc
     1021 gacgccgcga ggcagctgcc gcgcccgccg cactccgggc gaggcgggcg acgcgatgtg
     1081 gtgcgagccg gccgagcctg ggggacgctc cggagggagc gcgatgcgct cgtcgagggg
     1141 tccgtcggag aaggaacagg aggcagagag cccgagggga ccgcatccgg cgccccgagt
     1201 atcatcgcct gatggcagct cctcccgcga tccgcatcgg cgtctcctcg tgcctgctcg
     1261 gccaggcggt ccgcttcgac gggggccata aacggaaccc tctcctcgtc gagaccttcg
     1321 gaaggttcgt cgagtggatt ccggtgtgcc cggaagtgga gctgggcctc ggcgtgccgc
     1381 gcgaaacgct ccggctgacg cgcacggcgg acggcgaggt ccggatggtc accacgaaga
     1441 gcggccgcga catcacggcg cagatgcggg aatggtcgcg ggcccgcacc gaggcgctgg
     1501 ccggagaggg cctctcgggg tacgtcctca agaaggactc gcccagctgc ggcatggagc
     1561 gggtgacagt gtccggcggc tccggcgcgg cgctccggac gggccggggg ctgttcgccg
     1621 aggcgctgat ggcgcgcttt ccccgcctgc ccatcgagga cgagggacgg ctgtcggatc
     1681 cccggctccg cgacaacttc gtcgagcggg cctttgccta ccgtcgtctg ggcgcgctgt
     1741 tcgccgggcg atggcagccc gacgacctga ttcgcttcca cgccgcgcac gaactggcgc
     1801 tgctggctca ttcgccggcg atcgaggcgg agctggcccg cctggcggcg cgggggacgt
     1861 caatggcgcg ggccgacctc gagatccgct atcgcgatcg tttcatggat gccatggcca
     1921 cgctcgccac cacgcgcaag cacacgaacg tgctgcggcg catggccagg cgcctcgcgc
     1981 ccctcctcgg ggcggacgct tggcaggagc tgcaatcgaa tctcgaggag taccggctcg
     2041 gcctggcgcc gctcgtcgtg cccctggcgc tcgtcaggcg ccaggtcagg cgccaccgcg
     2101 tcgagcagct cgccggccag gtctacctgg atccgcaccc gactgaactg atgctccgga
     2161 accacgtgta gccgcccggc tcccggggcc cgcccgcggg gacccgcgca gcggcggcaa
     2221 ccggccgcgg cggatgggcg ttcgggcgcc ggcgatggta cgatgggacg atgcgaggcc
     2281 gccggctcct ccaggtgatc ctgctcgccg cggcgctcct gccctacttc gtgaacctgg
     2341 gcgccacgtc gatcatcgac gcgaacgagg cgttctacgc gcagacgccg gtcgagatga
     2401 tggcgtcggg cgactgcatc agcccgacct tcaacggcga gccccgcctg aacaagcctc
     2461 cgctgtcgta ctgggcggtg gcggccagct acaaggtgtt cggcgtgtcg ctcttcgccg
     2521 cccgcctgcc gattgccctg ggcgccgtgg tcatcgtaat gaccgcgttt ttcctcggcc
     2581 agctcgcgtt ctcgccgggc gcgggcttcg tcgccgccgt cacgctggcg gcctccccgc
     2641 gcgtcctgct cttttcgcgg cggatcatca tcgacatcta cacgacgatg ttcgtgggcc
     2701 tggcgctcgc atgcttcgcg cgcgccgaga tcgagccgca gccggccagg cgccgcgcgt
     2761 ggctgctggc gatgtacgcg gccatcgggc tcggcgtgat gaccaagggc cctgtcgcca
     2821 tcgccctccc cggccttgtc ttcgccggct acctggcctt gatcggccgg ctgcgcaccg
     2881 tcacgcggct gatgctgcct acgggcgccg tcgtcgtcgc ggcaatcgtg gccccgtact
     2941 acgcggccct ctgcgcgcag gaaggctggg gcgcgatttc gtccttcctg cttcgcgaga
     3001 acctcgcgcg ctacacatcc ggcgtgggcg ccccggatcg aggccccctg ttctacctgc
     3061 ccgtcgtgct cggcgacttc tactttccgt ggtcgctgct gctgcccgcg gccttcgcgc
     3121 tcgtgccgtg gcggtccgtc ctcgccgggc ggggatgggc caggatctgg ggcggtcccg
     3181 gcgccgaggg tccggttgcg cccgaacgcg tgcggctcct gctcggcctg tggatcatcc
     3241 tcatcgtggc cttcttctcg ctgtccaacg gccagcagga cctctacgtg ctgccgttcg
     3301 tgacggcggc cgcgccgctg gtcggagggc tcgtggcggc gtgggcgggc agcaggatca
     3361 cggggcggct ggaccgagtc gccacctgga gcgtcgcggc cattgccgtg gcgctcgccg
     3421 cggccggcgc tggcgtcgcc tggttcgtgg gccggcccgg cgagcggatc catctggcag
     3481 gggcgctcgc gacgggcggc gtcctgatca tcggaggcgc cgccgctctg gcggcgctga
     3541 aacggcacgc gcgcgccgtc gcggcgatcg gcctggccgc ggccgccatc ctcagcctct
     3601 ggatcatcgt catccgatcg ctgcccgact acgagcgcta ccagccggtt ccgcagcttg
     3661 cgcgggtcat ccgggcaggc acggggccgg cgccggccgt ggccacatac ctggtggccg
     3721 cgccgagcct cgtcttctac ctgaaccgcc acgtccacca gctcttcgat cgagagcagc
     3781 ttcacgcgtt cctgcgcgcc aaccccagcg gcctgtgtct catgacggac gacgagtacg
     3841 cggccgtgag agaaagcctg ccggtgccga cgcacgtcgt cgcgagcgcc ccgaggatgg
     3901 gctcgaagct ggtggatttc ctcgaccggg caccgctgcg aatgctcgtg ctgatcggcc
     3961 ctgcctgagc tcctcgcgcg cacggcgagg gtgcgctggg cgcgccgatt ccaatcgtca
     4021 attgattgac agcgtccgca agtgccggac gtccggcgat aaatccggca gatgacggaa
     4081 ttccgatgat tccgttcgcg gaatagtccc taacaaatgc gaaatccggc cggaaacaac
     4141 gccaagcgct gcaaccgatt cctggcaccg ctattgcgat aacaagcggc atgagcccat
     4201 cggttgggct ctggcggcaa cggatggggg cccgttgccg ttggggtatc aaccgatggg
     4261 ctgttttctt gtacgagcgt cagtccctgt cctcctcgtc gtcatcgtct ccgggcagcg
     4321 cacgaacgaa cgcggcccgc acgggaggcg gcatcggatg gttcgccccg cggacggagc
     4381 gccagacgat gtcgttcaac tcgaggtcgg ggcagagatc cgcttcggcc atgttcatgg
     4441 ccgccgaggc cgcggccccc caggcattga cggcgttctt ctccgtcacg tcgatgcgcg
     4501 ccggacgcgc ccggtagggc gtgagcaccg gcgtggcctg gaacgcgttg tacatcggcg
     4561 tggccgcggc gtcgtagttg ctcatcgggg gcaggccgag gatgagttcc atcgtccgca
     4621 gcatgcccga ggtcgagtac atcgtgctgt ccacgacgcc gcgcttgacg aaggggctga
     4681 tgacgaatgc cggcgatcga tgcgcgtcca cgtggtccgg cccgttctgc gcatcgtcct
     4741 ccaggacgaa gatcgccgac tccttccagt acacgctgcg gctgacggcc tcgacgatcc
     4801 ggccgagcgc cgcgtcgttt tcggcgatca tcgcgcgcgg cgtcggatag ccggcccgcg
     4861 tgcccgccgt atggtcgccg ccgatgcgga ggatgctcag cgccggcagc ccgccgtcct
     4921 tctcgaaccg gcggaactcc tcgagccaga cgtccacgcg cacgttgtcc ggcaccgaca
     4981 ggtcccaggg gggat
//
LOCUS       DAOSIF010000198         4955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15164_length_4955_cov_3.850612, whole genome shotgun sequence.
ACCESSION   DAOSIF010000198 DAOSIF010000000
VERSION     DAOSIF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15164_length_4955_cov_3.850612"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1279)
                     /locus_tag="PLT35_07720"
     CDS             complement(38..1279)
                     /locus_tag="PLT35_07720"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOG29152.1"
                     /translation="MAARARLSRTPISRRAVPLRAAASAAGAPLMAYVGTFSSPHRGV
                     PRAQEGQPPGNGCGIHLFQVARETGAFAPHGTVDLGTSPSCLAVNAAGTRLYSTNETD
                     RVGASAAGSVSAFVLDRADGRLSELNTVSSGGAGPTHLSVHPSGRFLLVANYLGGSIA
                     VLPILADGRLGRAADVKRGAGAVGPRRAPSAPPGSFAWGGHDRAHAHMVQADPSGRFV
                     LHADLGLDRIFVWKLDERTGVLAPADTREMALPPGDGPRHFAFHPSGRWLYSIQEEGS
                     TIALFDYDGATGGLAPRQTVSSLPPGFAGSSYGSGILVSADGRFVYAGNRLHDSISVF
                     AVGRAGELTFAGSEWTRGDYPRSFAFDPAGRFLCCCNQRADNVTVFRVDSSTGALAFT
                     GQYVPVGSPSGIVFVDLAEAG"
     gene            1615..2598
                     /locus_tag="PLT35_07725"
     CDS             1615..2598
                     /locus_tag="PLT35_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG29153.1"
                     /translation="MTLDDNGLTRRGFFQGVMGAAVAAAFAPGPAQADTARARVVRVE
                     SPGVWKGDARRPEVVAAMIGAGLMSFTGESKADAAWRRFVKPGMRVGVKLNLLGRPLV
                     ITAREVTDAVVSGALAAGVRPEDLTVWDRKPEHFGPDTYQPGTGRHGERIKTGSVYHD
                     TIAADTSGGKAPIDRIPIEETDVTINLPVIKDHGMAGVTGALKNIAFGCYRNTRTAHS
                     GNCDPYIAETCEHFYRVARVPLIVLDATEGCFDQGPAPSNSQAVWRENAIYVATDPVA
                     LDVVMRDVIHAKRVAAGLPDVMRRSRHIETAAGAGLGVGDRARIDLVTVRV"
     gene            2842..4218
                     /locus_tag="PLT35_07730"
     CDS             2842..4218
                     /locus_tag="PLT35_07730"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOG29154.1"
                     /translation="MTRWIAALTALAAAAGPAVAEDGWRPLFNGKTLDGWAQLNGTAP
                     FTVADGAIVGTTVVDSPNSFLCTTEEFGDVILELEAKLDAPMNSGVQFRSLSTPAYMD
                     GRVHGYQVEIDPSDRAWTGGIYDEARRGWLHPVYGQEAARRAFRMHEWNRFRVEAIGP
                     SLRTWVNGVPVANVVDDLTPRGFIGLQVHSIGRDASRAGETVRFRNIRVLTANLAAHR
                     TPDRGDTPQFNFIPNTLTERERQEGWRLLWDGSTTAGWRGARLRTFPEKGWTVTDGAL
                     SVAETGGGEAAAGGDIVTEDEYGDFELIVEFNLTKGANSGIKYFVDTDLNQGAGSSIG
                     CEYQLLDDASHPDAALGVQGNRTLAGLYDLIPPVGVRFNGVGQWNRARIVVRGARVEH
                     WLNGFKTVEYERGTQMWRALVSHSKYTVWPGFGERPRGRILLQDHGNAVSFRSIKIRE
                     LDPRAPAR"
     gene            4269..>4955
                     /locus_tag="PLT35_07735"
     CDS             4269..>4955
                     /locus_tag="PLT35_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29155.1"
                     /translation="MPSTPHVTRRRFVKTTVAGSAGLMLASPLGRESLLFGQSPNRRI
                     QIAQVGCGRMGTEDLLGTIRHADLCRIVAVCDLDSKRLAAAKAKAEQFYRERGERAVD
                     VKAYRDYREVLARPDIDAVSVTSPDHWHAIVAVEAALAGKDLHVQKPLTYDINEAIAL
                     RTAVRARKRILQTGSQQRSSNPWNTFRVATEAVRNGRIGRIHTVKVGIGQDQPKGQPP
                     AAQPVPPNLDY"
BASE COUNT          777 a         1767 c         1786 g          625 t
ORIGIN      
        1 aaggcgcggg ctggcgcggc gcccgccggc gcccgcgcta gcccgcttcc gcgagatcga
       61 cgaacacgat gccggacggg ctgcccaccg gcacgtactg ccccgtgaaa gccagcgcgc
      121 cggtggacga atccacccgg aacacggtca cgttgtcggc ccgctgattg cagcagcaga
      181 ggaaccggcc tgcgggatcg aacgcgaagc tcctggggta gtcgccgcgc gtccactcgc
      241 tgccggcgaa ggtcagctcg cccgcgcggc cgaccgcgaa gacgctgatg ctgtcgtgca
      301 gccggttccc ggcatacacg aaccggccgt cggccgacac gagaatcccc gagccgtagc
      361 tgctgccggc gaaccccggc ggcaggctgg acacggtctg ccggggcgcg agcccgcccg
      421 tcgcgccgtc gtagtcgaag agcgcgatgg tcgagccctc ttcttggatc gaatagagcc
      481 agcggccgct tggatggaac gcgaagtgcc tcggcccgtc gccgggcggc agcgccatct
      541 cgcgcgtgtc ggcgggcgcg aggacgcccg tccgctcgtc gagcttccac acgaagatcc
      601 ggtcgaggcc gagatcggca tggagcacga accgccccga tggatcggcc tggaccatgt
      661 gcgcgtgcgc ccggtcgtgc ccgccccacg cgaagctccc gggcggcgcg ctgggcgccc
      721 gccggggccc caccgcgcct gcaccccgct tgacatcggc ggcgcgccca agtcgcccgt
      781 cggcgaggat cggcagcacc gcgatcgatc cgccgaggta gttggcgacg agcaggaacc
      841 gtcccgacgg atgcacgctg aggtgggtcg gccccgctcc gcccgacgac acggtgttca
      901 gctcggacag tcggccgtcg gcacggtcga ggacaaaggc gctcaccgag ccggccgccg
      961 acgcgccgac ccggtccgtc tcgttcgtgg agtagagccg cgtgccggcg gcgttgaccg
     1021 cgagacagct cgggctggtc cccagatcga cggtgccgtg cggcgcgaac gcgccggtct
     1081 cacgagccac ctggaacagg tggatgccgc agccgttccc cggcggctgg ccttcttgcg
     1141 cccgcggtac gccgcgatgc ggcgagctga aggtgcccac gtacgccatg agcggggcgc
     1201 cggcggcact cgccgcggcg cgcagcggga ccgcgcggcg ggagatggga gtccgcgaca
     1261 ggcgcgcgcg agcggccatg gcatgcgcat cgtaccagcc gcgggcgcct cgcgccactc
     1321 gttgtgcacc cgcgggttcg cggcgaagtc ggaaggccgc ggccggctcc ggccgcccga
     1381 gcccacgcac cgcggctccc tgcgccgcgc cactccgggt cgagcgcggg gtgcccaagg
     1441 agagcctcgc ctgccgcggc gcacttgcga agcccgcagg ccttcgaccg cggcatcctg
     1501 cggaatcagc aggcggcagg cgggccgggc cggtttgctg ctagactctc tgaaccccgc
     1561 ccgggagggc ggaccggccc cgggcatccg gcctggcagc gaaggagtcc aacgatgact
     1621 ctcgacgaca acgggctgac gagacgcggg ttcttccagg gcgtgatggg agcggcggtg
     1681 gcggcggcat tcgcgccagg acccgcgcag gccgacacgg cgcgggcgag ggtcgtccgc
     1741 gtggagtcgc cgggcgtctg gaaaggcgac gcaagacggc ccgaggtcgt ggcggccatg
     1801 atcggcgccg gcctcatgtc cttcaccggc gagtcgaagg ccgacgcggc gtggcggcgc
     1861 ttcgtcaagc ccggcatgcg cgtgggcgtc aagctcaacc tgctgggccg acccctcgtc
     1921 atcaccgccc gcgaggtcac agatgccgtc gtgtccggcg cgctcgccgc tggcgtgagg
     1981 cccgaggacc tcacggtgtg ggatcgcaag cccgagcact tcggcccgga cacctaccag
     2041 cctggcacgg gcaggcacgg cgagcgcatc aagaccggct ccgtgtacca cgacacgatc
     2101 gcggccgaca cgagcggggg caaggcgccc atcgaccgga tcccgatcga ggagaccgac
     2161 gtcaccatca atctgccggt catcaaggac cacggcatgg cgggcgtgac cggcgcgctc
     2221 aagaacatcg cgttcggctg ctaccggaac acccggactg cgcacagcgg gaactgcgac
     2281 ccgtacatcg ccgaaacgtg cgagcacttc taccgggtcg ccagagtccc gctgatcgtg
     2341 ctggacgcca ccgagggatg cttcgaccag gggccggcgc cgtcgaactc gcaggcggtc
     2401 tggcgcgaga acgccatcta cgtggccacc gatccggtgg ccctcgacgt ggtgatgcgg
     2461 gacgtgatcc acgcgaagcg tgtcgcggcg ggcctgcccg acgtgatgcg ccggagccgt
     2521 cacatcgaga cggccgccgg agcgggcctg ggcgtcggcg accgcgcgcg gatcgatctc
     2581 gtcacggtca gagtctagcg cctctgcggg cgcggccgcc cccgaggcgg cggagggcag
     2641 cccggctgtc tgcatccgcg accgcgcgca cgacgccggc gtgccgtcga cgggcggccc
     2701 cagccgcctg ttggcgcccc gccgccgcgt tgacactccc tctcgcctcg tgtgtacttg
     2761 tcgggccggg tccgagcgcc agcctggcgc acccggtcga cggcgctcca ggcgcgaacg
     2821 ggcatcttcc cggaggactt catgacacgg tggatcgcgg ccctgaccgc cctcgcggcg
     2881 gccgcgggcc cggccgtcgc agaggacggc tggcggccgc tcttcaacgg caagacgctc
     2941 gacggatggg ctcagctgaa cggcacggcc ccgttcaccg tcgccgacgg cgccattgtc
     3001 ggcacgacgg tggtggattc gccgaacagc tttctttgca cgaccgagga gttcggcgac
     3061 gtcatcctgg agctcgaggc gaagctcgac gccccgatga acagcggcgt gcagttccgc
     3121 agcctgagca cgccggccta catggacggc cgcgtgcacg gctaccaagt cgagatcgat
     3181 ccgagcgacc gcgcgtggac cggcgggatc tacgacgagg cgcggcgcgg gtggctgcac
     3241 ccggtctacg ggcaggaggc cgcgcgcagg gcctttcgga tgcacgagtg gaacaggttt
     3301 cgcgtagaag ccatcgggcc ctcgcttcgt acgtgggtca acggcgtccc ggtggcgaac
     3361 gtggtggacg acctgacgcc gagaggcttc atcggccttc aagtgcacag catcggcagg
     3421 gacgcctcgc gggccggcga gacggtgcgc ttccgcaaca tccgggtcct gacggcgaac
     3481 ctcgccgcgc accgcacgcc cgaccgcggc gacacgccgc agttcaactt catccccaac
     3541 acgctcaccg agcgggagcg gcaggagggc tggaggctcc tttgggacgg cagcacgacg
     3601 gccggctggc ggggcgccag gctgcgcacg ttccccgaga agggctggac cgtcactgac
     3661 ggcgcgctga gcgtcgccga aacgggaggc ggcgaggccg ccgccggcgg ggacatcgtc
     3721 acggaggacg agtacggcga tttcgagttg atcgtcgagt tcaacctgac gaagggcgcg
     3781 aacagcggca tcaagtactt cgtggacacc gacctcaacc agggcgccgg gtcgtcgatc
     3841 gggtgcgagt accagctgct cgacgatgcc tcgcatcccg acgccgcgct gggcgtgcag
     3901 ggcaatcgga cgctcgccgg gctgtacgac ctgattcctc ccgtgggcgt gcgcttcaac
     3961 ggcgtgggtc agtggaaccg cgcccgcatc gtcgttcgcg gcgcgcgcgt cgagcactgg
     4021 ctgaacgggt tcaagaccgt cgagtacgag cgcggcacgc agatgtggcg cgcgctcgtc
     4081 agccacagca agtacaccgt atggcccggc ttcggcgagc ggccgagagg gcgcatcctg
     4141 ctgcaggacc acggcaacgc ggtgtcgttc cgcagcatca agatcaggga actggatccg
     4201 cgcgcgccgg ccaggtagcc gtaccgggcc cgtcgcacgc gggcgcaccg cccccgaaag
     4261 gatcggccat gccgtccaca ccgcacgtga cccgacgccg cttcgtcaag accaccgtag
     4321 ccggatcggc cggcctcatg ctcgcgtctc cgctgggccg cgagtcgctg ctgttcgggc
     4381 agtcgccgaa ccggcgcatc cagattgcgc aagtcggctg cgggcgcatg ggcacggagg
     4441 acctgctcgg caccatcagg cacgccgacc tctgccgcat cgtcgccgtg tgcgatctcg
     4501 actcgaagcg gctcgcagcg gccaaggcga aagccgagca gttctaccgg gagaggggcg
     4561 agcgcgccgt cgacgtgaag gcctaccgcg actaccgcga agtgctcgcc aggccggaca
     4621 tcgacgcggt cagcgtcacg tcgccggacc actggcacgc gatcgtggcc gtggaagccg
     4681 cgctggccgg caaggacctg cacgtccaga agccgctcac ctacgacatc aacgaagcca
     4741 tcgccctgcg cacggccgtg cgggccagga aacggatcct gcagaccggg agccagcagc
     4801 ggtcgtcgaa tccgtggaac accttccgcg tggcgaccga ggcggtgcgc aacggccgca
     4861 tcggccggat ccacacggtg aaggtgggca tcggccagga ccagccgaag ggccagccgc
     4921 ccgcggccca gccggtgccg ccgaacctcg actac
//
LOCUS       DAOSIF010000199         4951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15182_length_4951_cov_3.838031, whole genome shotgun sequence.
ACCESSION   DAOSIF010000199 DAOSIF010000000
VERSION     DAOSIF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15182_length_4951_cov_3.838031"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLT35_07740"
     CDS             <1..1227
                     /locus_tag="PLT35_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG29156.1"
                     /translation="RLALWLEHAVVAADAAGDRVRALKVRNLRTGNERWIEAPNVIDA
                     TELGDLLPLAGTEFVAGCEGRAATGEPHMPERADALNQQAFTAVFAMDFVPGSDQTIE
                     RPRDYAFWRDYAPRLTPPWTGRLLELAGSHPITLAPRRYAFDPTGAWQTDLTDLWRYR
                     RIANKDNFAAGAYAGDITVVNWPQNDYMLGPLVGVDDAARARHLDGARQLSLSLLYWL
                     QTEAPRPDGKAGWRELRLRGDVLGSEDGVAKHPYVRESRRIKALTTVLEQHCGTEARA
                     ALTGSAGATAERYPDSVGIGSYRIDLHPSSAGDNYIDVASLPFQIPLGALLPVRVENL
                     VPACKNIGTTHVTNGCYRLHPVEWNIGEAAGALVAFAAARRVPPRAVRKRPALLADFQ
                     ADLRKRGVELEWPPGA"
     gene            complement(1265..2452)
                     /locus_tag="PLT35_07745"
     CDS             complement(1265..2452)
                     /locus_tag="PLT35_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29157.1"
                     /translation="MATPLTVGLVGGGKGAFIVHAHQKAIHFDGTRRIVAGALHPDPK
                     VALDEAAAWPYPIKGYASYDEMIAANAGLPARERLDYVLIVTPNHVHYDPAMKAMQAG
                     IAVFCEKPLCLTLKEAADLVRTSRKLKIPFGVAHTYLGHWTSRFSRYIVRQGLIGDVR
                     WVDSAYLQGWLATKAEDTGNQQAVWRVDPRRAGGSGCGGDIGTHALMQLRYVTGLDVT
                     RVSAQLETFVQGRLLDDHFTVYCQLSNGGKALVRASQICIGCKNELGIMVAGTKGTLR
                     WRQEEPESVTIQLPGQPDRVYWRGAVTPGDGFLPKGVPKALLAEPTIPSGHPEGFHDA
                     FARLHREFEKDVRAWKAGKAFSGAGPQYASVEDGWTGIAFLDACLKSSRRKGAWVAMP
                     KAV"
     gene            complement(2711..4444)
                     /locus_tag="PLT35_07750"
     CDS             complement(2711..4444)
                     /locus_tag="PLT35_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29158.1"
                     /translation="MTNEPAQDSRARGGAADDLRVRAEQGQAAAQFALAFCREHGQAG
                     GQADPDAALGWYRKAAQQGHALAQCRLGLAYAQGRIVPRDDVAAVSWFQQAAEQGQPE
                     AAYMLGVMYGTGRGVGRDQNAAVLWCRKAALQGHPEAQFTLGVMHARGEGVPRDGWQA
                     VAWCRKAAASGHAEAQHTLGVMYANGDGVVKDAPQAVLWFRQAAEQGHAPSQVELGQA
                     FASGNGVEQDLVEAVAWFQRAAGQGHAAAERALAGLSGQAGAAPPAGPARPGPWAQDE
                     AAALPPEAPGSLSAGSVEAVGWPPAVHVPPAAPAEPSGWLVRAPEGVVAPPAEPPPPA
                     TPPPEDKRVASCRADAERGDRLAQYRLAAMYASGDGVAQDDSEAFAWCARSAEQGLPE
                     AQYNLAALYAAGRGVGRDEARALVWLRKAAEGGYPPAQFELGVHCATGREAPQDDSEA
                     AAWYRRAAERGHADAQYNLALRHARGRGVERDYALAAWWYRQAADAWHLPSQFNLGVR
                     YARGEGVPQDLVEAYWWIHLAATRSSGALNQSCQAALEELAARMTPAQAAARDRRLAD
                     WSEFPEQPSRR"
     gene            4760..>4951
                     /locus_tag="PLT35_07755"
     CDS             4760..>4951
                     /locus_tag="PLT35_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOG29159.1"
                     /translation="MASASGTIKRKVSDKGFGFIAAGDGKEYFFHQSACTQTPFDALR
                     EGDAVTFETSQGPKGPRAEN"
BASE COUNT          689 a         1818 c         1766 g          678 t
ORIGIN      
        1 agactcgcgc tgtggctcga gcatgccgtc gtcgccgccg acgccgccgg cgaccgcgtg
       61 cgggcgctga aggtccgaaa cctgcgcacg ggaaacgagc gctggatcga ggcccccaac
      121 gtcatcgacg cgacggaact gggggacctg ctgcccctgg cgggcaccga gttcgtcgcc
      181 ggctgcgaag gccgggccgc gacgggcgag ccgcacatgc ccgagcgggc ggacgcgttg
      241 aaccagcagg cgttcacggc ggtgttcgcc atggacttcg tgccgggatc ggatcagaca
      301 atcgagcggc cgcgcgacta cgcgttctgg cgcgactacg cgccgcgcct gacgccgccg
      361 tggacgggcc ggctgctgga gctggccggc agccacccga ttacgctcgc gccccggcgc
      421 tacgccttcg atcccaccgg ggcctggcag accgacctga cggatctctg gcgctaccgc
      481 cgcatcgcga acaaggacaa cttcgcggcc ggcgcctacg ccggcgacat caccgtcgtg
      541 aactggcccc agaacgacta catgctcggg ccgctcgtcg gcgtcgatga cgccgcacgg
      601 gcgcgccatc tcgacggcgc gaggcagctg agcctgtcgc tgctgtactg gctgcagacc
      661 gaggcgccga ggccggacgg caaggcgggc tggcgcgagt tgcggctccg cggggacgtg
      721 ctcggcagcg aggacggggt tgccaagcac ccgtacgtga gggagtcgcg gcgcatcaag
      781 gccctgacga cggtgctcga gcagcactgc ggcaccgagg cccgggcggc cctcacgggc
      841 tccgcgggcg cgacggccga gcgctacccc gattcggtcg gcatcggcag ctaccgcatc
      901 gacctgcacc cgagctcggc cggcgacaac tacatcgacg tcgcgtcgct gccgttccag
      961 atcccgctcg gcgccctgct cccggtgcgc gtcgagaacc tcgtgccggc ctgcaagaac
     1021 atcggcacga cgcacgtaac gaacggctgt taccgcctgc acccggtcga gtggaacatc
     1081 ggcgaggccg cgggcgcgct cgtggcgttc gcggccgcgc ggcgcgtgcc gccgcgcgcc
     1141 gtccggaaac gcccggccct gctcgccgac ttccaggctg accttcggaa gcgaggcgtc
     1201 gagctggagt ggccgcccgg ggcctgacgg ccccaggctt catccgccgc cgccgtgccg
     1261 ggcgtcagac ggccttgggc atcgcgaccc aggcgccctt ccttcggctg ctcttcaggc
     1321 acgcatcgag gaacgcgatg cccgtccagc cgtcctcgac gctcgcatac tgcgggccgg
     1381 cgcccgaaaa cgccttcccc gccttccacg cgcgcacgtc cttctcgaac tcgcggtgca
     1441 gccgcgcgaa cgcgtcgtgg aagccctcgg ggtggccgga gggaatcgtc ggctcggcga
     1501 gcagggcctt ggggacgccc ttcgggagga acccgtctcc cggcgtgacg gcgccacgcc
     1561 aatacacgcg gtccggctgg cctggcagct ggatcgtcac gctctcgggc tcctcctgcc
     1621 gccaccgcag cgtgcccttc gtgccggcga ccatgatgcc cagctcgttc ttgcacccga
     1681 tgcagatctg cgacgcgcgc acgagcgcct tcccgccgtt cgagagctgg cagtagacgg
     1741 tgaagtgatc gtcgagcagc cgcccctgca cgaaggtctc cagctgcgcc gagacgcggg
     1801 tgacgtcgag gccggtcacg tagcgcaact gcatcagcgc gtgggtgccg atgtctccgc
     1861 cgcacccgga cccgccggcg cgcctgggat ccacgcgcca caccgcctgc tggttgcccg
     1921 tgtcctcggc cttcgtcgcc agccagccct ggaggtaggc gctgtccacc cagcgcacgt
     1981 ccccgatgag cccctgccgg acgatgtagc gcgagaaccg gctggtccag tgcccgaggt
     2041 acgtgtgcgc gacgccgaag gggatcttca gcttcctgga cgtccggacg aggtcggcgg
     2101 cctccttgag cgtgaggcac agcggcttct cgcagaacac ggcgatgccg gcctgcatgg
     2161 ccttcatggc ggggtcgtag tgcacgtggt tcggcgtgac aatgaggacg taatccagcc
     2221 gttctcgggc cgggaggccc gcgttggcgg cgatcatctc gtcgtacgac gcgtagccct
     2281 tgatgggata cggccaagcc gctgcttcgt cgagcgcgac cttcgggtcc ggatgaagcg
     2341 cccccgccac gatgcggcgg gtgccgtcga agtgaatcgc cttctggtgg gcgtggacga
     2401 tgaacgcgcc cttgccgccg ccgaccagtc cgacagtgag gggtgtcgcc atggcctgtt
     2461 gtctccgtgt cgttggtccc ggtccttcac aggcggccgt ccggatcccc gcgccggcgc
     2521 cggccgccgc ttgccgatgg ggcaaagcgt acaccaggac gcgccgaccg gcgcccgtgc
     2581 gcgcgcaggg gggcggccgc ggagcgcacg ggagaccggc ggcatgagtc gtccggccga
     2641 cagcgaggcg acgatgttgt caggcgaacg cccgatttcg tctccggctg ctccgcaggc
     2701 gcggtccgcc ctacctccga gacggctgct cggggaactc gctccagtcc gccaggcgcc
     2761 tgtcgcgcgc cgccgcctgc gcgggagtca tccgggcggc gagttcctcc agcgccgcct
     2821 ggcaggactg attcagcgcg ccggacgacc gggtggccgc gaggtgaatc caccagtacg
     2881 cctcgaccag gtcctgcggc acgccctcgc cgcgcgcgta gcggacgcca aggttgaatt
     2941 gcgaaggcag gtgccacgcg tcggcggcct ggcggtacca ccatgccgcg agcgcgtagt
     3001 cgcgctcgac gccgcggccg cgcgcgtgcc tgagcgccag attgtactgc gcgtcggcgt
     3061 ggccgcgctc ggcggccctg cggtaccacg cggccgcttc gctatcgtcc tggggagcct
     3121 cgcggccggt ggcgcagtgg acgccgagct cgaactgcgc cggcgggtag ccgccttcgg
     3181 ccgccttccg cagccacacg agggcccgcg cctcgtcgcg tccgacgccg cggcccgcgg
     3241 cgtagagcgc ggccagattg tactgggctt ccggaagacc ttgctcggcc gacctggcgc
     3301 accaggcgaa ggcctcgctg tcgtcttgcg caacgccgtc gccggacgcg tacatggccg
     3361 ccagccggta ctgggcgagc cggtcgccac gctcggcgtc ggcgcggcac gaggccacgc
     3421 gcttgtcctc cggcggcggc gtggcagggg gcggcggctc ggcgggcggc gcgacaacgc
     3481 cttccggcgc gcggaccagc cagcccgacg gctcggctgg cgccgccggc ggcacgtgca
     3541 cggctggagg ccacccgacc gcttcgacgc ttccagccga aaggcttcct ggggcctccg
     3601 gcggcagcgc ggcggcctcg tcctgcgccc acggacccgg acgcgcaggg ccagccggag
     3661 gcgcggcccc agcctgcccc gacaatccgg ccagcgcgcg ttcggccgcg gcatgccctt
     3721 gcccggcggc ccgctggaac cacgcgaccg cctccacgag atcctgctcg acgccgttgc
     3781 cggaggcgaa ggcctggccc agctcgacct gggacggcgc gtggccctgc tcggccgcct
     3841 ggcggaacca gaggaccgcc tgaggggcgt ctttcacgac gccgtcaccg ttggcgtaca
     3901 tcacgccgag ggtatgctgg gcctcggcgt ggcccgatgc ggccgccttc cggcaccagg
     3961 ccacggcctg ccagccgtct cgaggcaccc cctctcccct ggcgtgcatc acccccagcg
     4021 tgaactgcgc ctccggatgg ccctgcagcg cggccttgcg gcaccacagg acggcggcgt
     4081 tctggtcccg accgactccg cggccggttc cgtacatcac gccgagcata taggcagctt
     4141 ccggctggcc ctgttcggcg gcctgctgga accacgacac ggccgcaacg tcatcgcggg
     4201 gcacgatgcg gccctgggcg tacgcgaggc cgaggcggca ctgggccagg gcgtgcccct
     4261 gctgggcggc cttccggtac cagcccagcg cggcgtcggg atcggcctgg ccgccggcct
     4321 gtccatgctc gcggcagaac gccagggcaa actgggcggc tgcctggccc tgctcggccc
     4381 ggacgcggag atcgtccgcg gcccctccac gcgcccggga atcctgcgcc ggttcgttgg
     4441 tcacgggctc tgcctccagc ctgccggcac acaccacgtg caaagagtct acccgatgcc
     4501 ctgcctgggg acttccggcg gaggagcccc tcgtcgaggg cgcctgatgg ccccggtcgg
     4561 gggccgacgc ggcgcccggc cgggcgcctc ggcagagaag gcgcgaagaa acggagaaaa
     4621 acgtgctacc ctaatcggga gcgtgcgcga ccaagcgacg agagcctgtg cagttacgca
     4681 cagctcgcgt gccctcaccg tctctatcgt ggcgacgaag agtggtgcag catcgaaggc
     4741 ggtcgcacga ggagacgaca tggcgagtgc aagcgggacg atcaagcgga aggtcagcga
     4801 caagggcttc gggttcatcg ccgccggcga cgggaaggag tatttcttcc accagtcggc
     4861 gtgcacgcag acgcctttcg acgcgcttcg cgaaggcgat gcggtgacgt tcgagaccag
     4921 ccagggcccg aagggcccgc gggccgagaa c
//
LOCUS       DAOSIF010000200         4950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15185_length_4950_cov_3.549949, whole genome shotgun sequence.
ACCESSION   DAOSIF010000200 DAOSIF010000000
VERSION     DAOSIF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15185_length_4950_cov_3.549949"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1328
                     /locus_tag="PLT35_07760"
     CDS             114..1328
                     /locus_tag="PLT35_07760"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29160.1"
                     /translation="MRWGTVAGLAATWLLLAVLVQRQFRGPSVNLDPSTVWLYARTQA
                     EVIVHYLRLAFVPSPLVFLYDWALMPAPLWRAWQAAVVLALLALTAAGLVGRHPASFL
                     GAWFFLILAPSSSVLPIITEVAAEHRMYLPLASVVAGVVVGLFLLGQRLVPSSSAASR
                     LTAARASAAIVLAAAVVALGLGTRARSHVYASAVGLWQDTVEKRPEDARPRIAYGEAL
                     AAAGRFAEAEAELRKGTELAPQDAFALVRLGSVLAQQRKYDEAVSHLVTALAVSPGNF
                     DAHRFLADIRALRGEDESAVEHYEAALATVPADARVLTALAAVLAGSRDPNVRNPARA
                     RGYAERAVAATGGQDARALQALSAAQAADGSLAEAARTARSAAAVARARGEIALADAL
                     EYRALSYESVRK"
     gene            complement(1501..2169)
                     /locus_tag="PLT35_07765"
     CDS             complement(1501..2169)
                     /locus_tag="PLT35_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG29161.1"
                     /translation="MGHKLASGVVLVTVAIFGASACATKGYVNKNVADVNTKVESLTK
                     SLEEAQERTKANEGKINVVDQKVGAVQGSADAARKAAADADAKAVAADAKVATLDKAS
                     RKLVYSVALSADQANFAVGRTELPEGSKAKLDELATRVKNDGQLVFFEIEGHTDSTGP
                     KELNYRLGLERAEAVKMYLHDQHQIPLHKMSVISYGPDKPVAPNKTRADRAQNRRIVV
                     NVLQ"
     gene            complement(2183..3142)
                     /locus_tag="PLT35_07770"
     CDS             complement(2183..3142)
                     /locus_tag="PLT35_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG29162.1"
                     /translation="MRRGAIAVLSLFMAGLSSAGDLVSAQAGQALPAKTTFRSGVDLV
                     RVSATVRDQRGRPVLGLTRHDFQLNVNGERVPILAFQSDASGVSLAVLVDSSGSMKLA
                     GRMAAARDIVGHVVRWVEPGRDEVALLSFDRNLRTVHPFTSDPLSVLPHLDALQPYGM
                     TSLYDAIAEAARPLTGRQRPRRAVLVVTDGIDNGSELTPPEVSRIASEIDVPVYVVAV
                     RVGADRIGGVPDETTVIDDASQATLLDLAAWTGGDAFFAGSPSETSQVARRILAELRH
                     QYLIGFQASARTGWHPLSIGVRKKNLSVRARGGYVTGPSVGSQ"
     gene            3486..4292
                     /locus_tag="PLT35_07775"
     CDS             3486..4292
                     /locus_tag="PLT35_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29163.1"
                     /translation="MSMKPIGLAAAVWLALGAAPQDPLVRARQLYNEHQYEAAAVAAR
                     EASRVPELADVAAVVLARAHLECYRASGEAADLLAARQALVAADSRRLAPRDRHDLLV
                     GYGELLYFDGHYGAAAEMFGAALSSAGQAAPADRDLVLDWWAAALDREAQLTTGADRE
                     RLYRRVIDRMEPAAEADPPSAAALYWLAAAAAGAGDAGRAWHAAVSGWIRAPLAVGPR
                     ATLRADLERLVQQAIIPLRARLPTAGGDARQAAAAMQRDWEAIKEIGIRR"
     gene            complement(4305..4880)
                     /locus_tag="PLT35_07780"
     CDS             complement(4305..4880)
                     /locus_tag="PLT35_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29164.1"
                     /translation="MTRHVTGLALAMAAAAALVTACATAQAKIDPAAGPVLAPPAPPP
                     RAVEPVELPQGTPDAEPEPGPVPVVIKPAPRPTVSRPDRNSGKATEKADQAGAAAAPP
                     PVAPGPGSAPLQTTANVMEAERTVRALMARAAHDLDRTDTRALSGDRRMQYDTARRFL
                     QQAGDALEVKNYVFAEQLADKAATLAAALVK"
BASE COUNT          647 a         1772 c         1798 g          733 t
ORIGIN      
        1 gcatgccctc gtggtggtag acgtaggcgg ggaagacgat ggtgaccgcg ccggtcgccg
       61 tggcgatctg ggaccgcctg ttccgccagc cggacacgtc cggacgcggc cgcgtgagat
      121 ggggcacggt ggcgggactg gcggcgacgt ggctcctgct ggccgtactc gtccagcggc
      181 agttccgggg gccgtcggtc aatctcgacc cgtcgacggt ctggctctac gcgcgcaccc
      241 aggcggaggt gatcgtgcac taccttcggc tcgcgttcgt gccgtcgccg ctggtgttcc
      301 tctacgactg ggcgctgatg cccgcgccgc tgtggagggc ctggcaggcg gccgtcgtgc
      361 tcgcgctgct cgcgctgacg gcggccgggc tcgtcggcag gcacccggcc tcgttcctgg
      421 gcgcgtggtt cttcctgatc ctggcgccct cctccagcgt gctgcccatc atcaccgaag
      481 tggcagccga gcaccgcatg tatctgccgc tggcgtcggt cgtggcgggc gtggtggtcg
      541 ggctgttcct gctcggacag cgcctcgtgc cctcatcctc tgccgcgtcg cggctgacgg
      601 cggcacgcgc ctccgccgcc atcgtcctcg ccgcggccgt ggtcgcactg ggcctcggca
      661 cccgcgcccg cagtcacgtc tacgcgagcg cggtcggcct gtggcaggac acggtggaga
      721 agcgcccgga ggatgcgcgg ccgcgcatcg cgtacgggga agcgcttgcg gcggcgggcc
      781 ggttcgcgga ggcggaggcc gagctgcgca aggggacgga actcgcgcct caggacgcct
      841 tcgcgctggt gcggctcggc agcgtgctgg cgcagcagcg caagtacgac gaggccgttt
      901 cgcacctggt gaccgctctg gccgtcagcc ccggcaactt cgacgcgcac cgcttcctcg
      961 ccgacatccg cgccctgcgc ggcgaagatg agtcggcggt ggagcactac gaggccgcac
     1021 ttgcgacggt gccggccgat gcgcgcgtgc tgacggcgct ggcggcggtc ctggctggat
     1081 cgcgggaccc gaacgtgcgg aacccggcgc gggccagggg gtacgcggaa cgggccgtgg
     1141 cggcgacggg cggacaggac gcacgcgccc tgcaggcgct ctcggccgcg caggcggcgg
     1201 acgggtcact ggccgaggcc gcgcgtaccg cacggtcggc cgcggcggtg gccagggccc
     1261 ggggcgagat tgccctggcc gacgccctcg agtaccgcgc tctctcgtac gagagcgtgc
     1321 ggaagtagcg ctgccggccg aacaggagcc ctgttcggcc gtgaaacgcg ggacgacagg
     1381 ggcggagggc accccccggc ctccagcggc cgggcctcga ccgagagccc gcctggcgga
     1441 cgcggaaact gggcgcgtgc ggaggcgcac gcggcgcctc cgcacggtcc ccgacgccgg
     1501 ctactgaagg acgttgacga cgatccgccg gttctgcgcg cgatcggccc gcgtcttgtt
     1561 cggcgcaacg ggcttgtccg gcccgtagct gatcacgctc atcttgtgca gcgggatctg
     1621 gtgctgatcg tgaaggtaca tcttcacggc ctcggcgcgc tccagcccga gcctgtagtt
     1681 gagctccttc ggaccggtcg agtcggtgtg gccttcgatc tcgaagaaca ccagctggcc
     1741 gtcgttcttc acgcgagtgg ccagctcgtc cagcttggcc ttggagccct ctggcagctc
     1801 ggtcctgccg acggcgaagt tggcctggtc ggccgacagc gccacgctgt agaccagctt
     1861 gcgcgacgcc ttgtcgagcg tcgcgacctt cgcgtcggcc gccacggcct tcgcatcggc
     1921 gtcggcggcc gccttgcgcg ccgcgtcggc tgagccctga accgctccga ccttctggtc
     1981 gacgacgttg atcttgcctt cgttggcctt cgtgcgctcc tgcgcctcct cgagcgactt
     2041 ggtcagcgac tcgaccttgg tgttcacgtc cgcgacgttc ttgttgacgt atcccttcgt
     2101 ggcgcacgca gacgccccga atatcgcgac ggtgaccaac acgactcccg atgccagctt
     2161 gtgacccatc tccactcctt tgctactgtg aaccgacgct ggggcccgtc acgtagcctc
     2221 cccgggcccg cacagacagg ttcttcttgc gcacgccgat cgacagcgga tgccagccgg
     2281 tgcgggcgct cgcctggaac ccgatcaggt actggtggcg cagctccgcg aggatgcggc
     2341 gcgccacctg gctcgtctcg gacggcgacc cggcgaagaa tgcgtccccg ccggtccagg
     2401 cggccagatc cagcagcgtc gcctgggacg catcgtcgat gacggtggtc tcgtccggca
     2461 cgccgccgat gcggtcggcg ccgacgcgga ccgccacgac gtagaccggc acgtcgatct
     2521 cgctcgcgat ccgcgacacc tcgggcggcg tcagttcgct gccgttgtcg atgccgtcgg
     2581 tcaccaccag gacggcccga cgcggccgct ggcgcccggt gagcggccgc gcggcctcgg
     2641 caatcgcgtc gtagagcgac gtcatgccgt acggctgcag cgcgtcgagg tgcggcagca
     2701 cggagagcgg gtcgctcgtg aacggatgga ccgtgcgcag gttccggtcg aagctgagga
     2761 gcgcgacctc gtcgcgcccc ggctcgaccc accgcacgac gtgcccgacg atgtcgcggg
     2821 cggccgccat cctgccggcc agcttcatgc tgccgctcga gtccaccagc accgccaggc
     2881 tcacgccgct cgcgtccgac tggaacgcga ggatcggcac gcgctcgccg ttcacgttga
     2941 gctggaagtc gtggcgcgtc agcccgagca ccggccggcc gcgctgatcg cgcacggtgg
     3001 ccgacacgcg cacgagatcg acaccggacc ggaacgtcgt cttcgctggc aaagcctgcc
     3061 ctgcctgagc ggaaaccagg tcgccggccg acgacagccc cgccatgaac agcgacagga
     3121 cggcgatggc cccgcgtctc acgccacgaa gtgaatcaag acccgtgcca gagaaggacg
     3181 gagcggacac gcgaaaaacg gccggaacgg acgcgggcac ggctgctcgc gcaccgggat
     3241 gtgtaagaac tacaccgtct gcgtgagcgc catatacttg ttgtccgacc ggcctgaccg
     3301 tggattggcg gagacgctcg gcttgtccag gaaggccgca cacacgcctg gcctccggag
     3361 cgttcgagac cggatccggc gggcggagcc gcctggcgca cactcggaaa aggacgggca
     3421 gcgggctggg ccgaggcgcg gggggttggg gcacttcggg cgcttgcgct actctatcac
     3481 cgcccatgtc gatgaagccg attgggcttg ccgccgcggt ctggctggcc ctcggggccg
     3541 ccccgcagga tccgctcgtc cgcgcgcggc agctctacaa cgaacatcag tacgaggccg
     3601 cagcggtggc ggctcgcgag gccagccgcg tgcccgagct tgccgacgtg gcggccgtcg
     3661 tgctcgcccg ggcgcacctc gaatgctacc gggcgtcggg ggaggccgcc gacctcctcg
     3721 ccgcgcgcca ggcgctcgtc gctgccgaca gccgccgcct cgctccgcgc gaccgccacg
     3781 acctgctcgt cgggtacggc gagctgctgt acttcgacgg ccactacggc gcggcggccg
     3841 agatgttcgg ggcggccctc tcgagcgccg gccaggcggc acccgcggat cgcgatctcg
     3901 tgctcgactg gtgggcggcg gcgctcgatc gagaggccca actgacgacc ggcgccgatc
     3961 gggagcggct ctaccggcgc gtaatcgacc gtatggagcc ggcagcggag gcagacccgc
     4021 cctcggctgc ggccctgtac tggctggcgg cagccgccgc cggcgccgga gacgccggcc
     4081 gcgcgtggca tgcggccgtc tccggctgga tccgcgcgcc gcttgccgtc gggccgcgcg
     4141 ccacgctgcg ggccgatctc gagcgcctcg tccagcaggc catcatcccg cttcgggccc
     4201 ggctcccgac ggctgggggc gacgccaggc aggcggccgc cgccatgcag cgcgactggg
     4261 aagccatcaa ggagattggc atccgccgct gacgcgccga ccggctactt cacgagcgcc
     4321 gcagcgagcg tcgcggcctt gtcggccagc tgctcggcga acacgtagtt cttcacctcc
     4381 agcgcatcgc ctgcctgctg caggaagcgt ctcgctgtgt cgtactgcat gcgccggtcg
     4441 cccgaaagcg cgcgggtgtc ggtgcggtcg aggtcgtgcg cggcgcgagc catcagggcg
     4501 cgcacggtcc gctccgcctc catcacgttc gccgtcgtct ggagcggggc cgagcctggc
     4561 cctggcgcaa ccggcggcgg cgcggcggcg gcaccggcct gatccgcctt ctcagtggcc
     4621 ttgccggagt tccgatcggg tcgggacacg gtagggcgtg gcgcgggctt gatgacgacc
     4681 ggcacagggc cgggttcggg ctcggcgtcc ggcgtccctt gaggaagctc gacgggttcg
     4741 acagcgcggg gaggaggcgc cggcggcgcg agaactggcc ctgcggcagg atcgatcttc
     4801 gcctgcgcgg tcgcgcacgc ggtgaccagc gccgcggccg cggccatcgc cagcgccagc
     4861 cccgtcacgt gccgtgtcat ggcctcatta tgcgccattg cggtcagatc cgcagcggtg
     4921 cggatccggc cccggttcag gcgcagggca
//
LOCUS       DAOSIF010000201         4931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15258_length_4931_cov_3.608285, whole genome shotgun sequence.
ACCESSION   DAOSIF010000201 DAOSIF010000000
VERSION     DAOSIF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15258_length_4931_cov_3.608285"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLT35_07785"
     CDS             complement(<1..302)
                     /locus_tag="PLT35_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG29165.1"
                     /translation="MDRELAARCRRGEAGAFEELYKRHGRRVYTLAYRMTGSAADAED
                     LTQDVFLRAHRRLDSYRGEAALGSWLHRLTVNACLDYARGKHGRRQRATDTTADIEA"
     gene            complement(439..756)
                     /locus_tag="PLT35_07790"
     CDS             complement(439..756)
                     /locus_tag="PLT35_07790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOG29166.1"
                     /translation="MTAPSFAMRVLHVVCRIPPGRAATYGDVAALAGSPRAARAVGTV
                     MRTCRAEGVPCHRVVAAGGRLGGYGGRPAMKRALLAAEGIIVIGARIADWRRVRWPRG
                     ARC"
     gene            complement(989..2380)
                     /locus_tag="PLT35_07795"
     CDS             complement(989..2380)
                     /locus_tag="PLT35_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG29167.1"
                     /translation="MSEDARRVRMAEALLRHFSACVKAVQLYAPEHPLVKRAVDQLAE
                     SFERAHVESEELVIGLIDHQAVVDGVPLLATPGVSETVERLQALGAERLAFRRGLTPD
                     ELLAFVRQLGARPALSGDRRDDRLAALSSPHVTVGRLTLQRQIDADCCDVAAMREHYG
                     QAVAGAEALWDQARAEGSPDPNAAIALVDGLASAIGENPRAMLALAALHRYDNYTFTH
                     MVNVSVLTMAQARSLGITGTLLRQFGLAGLMHDIGKIRTPAEILTKSDRLTDAEFAVM
                     MRHPLDGAEILRRRLDLPPLAAVVALEHHLRIDRTGYPAGVERSSLNLATQLCSIADV
                     YDAMRSQRAYQRAFPTDRIMAVLQQHDRKRFDDRLVRRFGQLMGVYPIGNIVRLDNGS
                     LAVVTRIHAPDPARPAVRVVVDPSGKRLDRPYDLALWTEEGRDGPPPRIVVPVDPDEA
                     GFDPLAYLDGAAA"
     gene            complement(2377..4644)
                     /locus_tag="PLT35_07800"
     CDS             complement(2377..4644)
                     /locus_tag="PLT35_07800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG29168.1"
                     /translation="MSEALSEPAAGAGPMPPDLANRIAAFARACRAAARAVALYPSDH
                     PALAAALEAVTAAAEEAAGGSALSLAVLPDALMVDGRRMLKPDPAAAEIASLLHRHQV
                     GLFSVHPHTTPEVWRLFLALLTLQPEQARLRGGLSRLWASEGETRIELRAVNYHELLR
                     VRSGGERATWEAIAAQCLEGLAFSLDDAIVEMLFGVLDDSSRIAGVVEAIESRLAPAG
                     AGGQTPAVVVSLLQAVAQFAAASDPEGIDPVLDALAAAMARLPLDTLGPVIGVSRGSN
                     RAGLARFVEGLTRRVSDGAIAALLAAEVRGGRGTSPALADAFCGLAPDPDRRAAIVTL
                     ARNLAATSEDAPSPSLAKAWQQSEAVLLAYSDRKFVSDAYNAELDRLAGRAVELYEDR
                     ADSPSLIAAWRETVGEDAVRLLDAALLADLLRLQPDLAHWRDLAALALQRLSVLLVVG
                     DFPAAVVLAETLRAHSNNHTDPGIRAAAEEMLQQVLTPSTMRHVASHLDTRDRPVVLA
                     AQRFCAAIGTAAIGPLADVLTREERSRPREHLISILAGFGAAGRQAAEQLRRSPNAAV
                     RRTAVQLLRRFGGQEALPELASLLDDIEPRVKREATHAIAMLGSEAAFDVLAQALTRD
                     DEVTRSAILGVLWTLPGEDAEPVLSHLARHAPCRGSMFEVHARLVGRLGMIGGAPSVD
                     ALAAVLERRRFWEPFRMAALHRAAIDALGRIGTAGARAVLEAAAGSGPRSVRGHARAR
                     LAESARRAEAGEQTA"
BASE COUNT          680 a         1824 c         1784 g          643 t
ORIGIN      
        1 gcctcgatgt cggccgtcgt gtccgtcgcc cgctggcgcc ggccgtgctt gccccgcgcg
       61 tagtcgaggc aggcgttgac ggtcaggcgg tgcagccagg agccgagggc cgcctcgccg
      121 cgatagctgt ccaggcggcg gtgggcccgc aggaacacgt cctgcgtcag gtcctcggcg
      181 tcggccgcgc tgccggtcat tcggtaggcc agcgtgtaca cccggcgccc gtggcgcttg
      241 tacagctcct cgaatgcgcc agcctcgccc cggcggcatc gtgccgccag ctcacggtcc
      301 atccggttgg cctccatggt gtcagacgga gtatgacgca tgactggttg cctgcccgct
      361 gagaaaaagc ctggagcgct cgtaccgaag ggtgacgtcc gggtgcggcg ccatgtttcc
      421 ccttgccacg ccagctgctc agcagcgtgc gccgcgcggc cagcgcaccc tgcgccagtc
      481 ggcgatgcgc gcgccgatca cgatgatgcc ttccgccgcc aggagcgccc gcttcatggc
      541 ggggcgcccc ccgtacccgc cgagcctccc gcccgccgcc accaccctgt ggcagggcac
      601 gccttccgcc cggcaggtgc gcatgaccgt tcccaccgcg cgcgcggccc ggggcgatcc
      661 agccagcgcc gcgacgtccc cgtacgtcgc ggcccgtcca ggcgggatgc ggcagacgac
      721 gtgcagcacg cgcatggcga aggagggcgc ggtcatctcc agctccaggc tgtcaggata
      781 cgcgcggccg ccgggatcgg caaccgcgca ccagccgtgt ggaccggcct cagcgaccgg
      841 ggccggcccg ccgcgcccgc aggcgtcggt tcggcgaaac cggacacgcc acgcgtcaca
      901 cgggaccgga gaactcgcgt gtcagcaaca ccgcaacgcc ggaccggcgg cggccgcccg
      961 gcgccgcggg gcggacgcgc gcagcaggct acgcggcggc accgtccagg taggcgagcg
     1021 ggtcgaagcc cgcctcgtcc ggatcgaccg gcacgacgat gcgcggcggg gggccgtcgc
     1081 ggccctcctc cgtccacagc gcgaggtcgt agggcctgtc cagccgcttg ccggacggat
     1141 cgaccaccac ccggaccgcc ggccgcgccg ggtccggggc gtggattcgc gtgaccacgg
     1201 ccagcgaccc gttgtcgagg cggacgatgt tgccgatggg gtagacgccc atcagctgtc
     1261 cgaaccgccg cacgagccga tcgtcgaagc gcttccggtc gtgctgctgc agcacggcca
     1321 tgattcgatc cgtcgggaag gctcgctggt aggcccgttg cgaccgcatg gcgtcgtaga
     1381 cgtcggcgat gctgcacagc tgcgtggcca ggttcagcga cgagcgctcg acgccggcgg
     1441 gataccccgt ccggtcgatc cgcaggtggt gctcgagcgc gacgacggcc gcaagcggcg
     1501 gcagatcgag gcggcggcgg aggatttcgg cgccgtcgag cgggtgcctc atcatcaccg
     1561 cgaattccgc gtcggtcagg cgatcggact tcgtcaggat ctcggcgggc gtccggatct
     1621 tgccgatgtc gtgcatcagg cccgccaggc cgaactgccg aaggagcgtg ccggtgatgc
     1681 cgaggctccg cgcctgcgcc atcgtgagga cgctcacgtt caccatgtgc gtgaaggtgt
     1741 agttgtcgta ccggtgcagc gccgcgagcg cgagcatggc ccgcgggttc tcgccgatgg
     1801 cgctcgccag gccgtccacg agggcgatgg cggcgttcgg gtccgggctg ccctcggccc
     1861 tcgcctgatc ccagagcgcc tccgcgccgg ccaccgcctg cccgtagtgc tcccgcatgg
     1921 ccgcgacgtc gcagcagtcc gcgtcgatct ggcgctgcag cgtcagccgt cccaccgtga
     1981 cgtggggcga cgagagcgcg gccaggcggt cgtcgcgccg gtctccggac agcgcgggcc
     2041 tcgcgccgag ctgcctgacg aaggccagca gctcgtcggg cgtgaggccg cgtctgaagg
     2101 ccaggcgctc cgcgccgagc gcctgcagcc gctcgacggt ctccgacacg ccgggtgtcg
     2161 caagcagggg caccccgtcc accaccgcct ggtgatcaat gaggccgatc accagttcct
     2221 ccgattccac gtgggcgcgc tcgaacgact cggcgagctg gtccaccgcg cgcttcacga
     2281 gggggtgctc cggcgcgtag agctgcaccg ccttgacgca cgccgagaag tggcgcagga
     2341 gcgcttcggc catccgcacg cgccgcgcgt cttcgctcat gccgtctgct ctcctgcctc
     2401 cgcccggcgg gccgattcgg ccagcctggc gcgggcgtgc ccgcgcacgc tcctgggtcc
     2461 gctcccggcg gcggcttcca gcacggcccg cgcgccggcc gttccaatcc ggccgagggc
     2521 atcgatggcc gcacggtgca gggccgccat gcggaacggc tcccagaagc gccggcgctc
     2581 gaggaccgcc gccaacgcgt cgacggaggg cgcgccgccg atcatcccga ggcgccccac
     2641 gagccgtgcg tggacctcga acatcgagcc gcggcagggc gcgtggcgcg ccaggtgcga
     2701 gagcaccggc tcggcgtctt cgccgggcag cgtccacaac acgcccagga tggccgaccg
     2761 cgtgacttcg tcgtcgcgcg tcagcgcctg ggccagcacg tcgaaggccg cctccgagcc
     2821 gagcatggca atcgcgtgcg tcgcttcccg cttcacccgc ggttcgatgt cgtcgagcag
     2881 cgaggccagt tccggcagcg cttcctgccc gccgaatcgg cgcagcagct gcaccgccgt
     2941 ccgccgcacg gccgcgttcg gcgaccggcg cagctgctcg gcggcctgcc ggcccgccgc
     3001 gccgaacccg gccaggatcg agatgaggtg ctcgcgcggc ctcgagcgct cctcccgcgt
     3061 cagcacgtcg gccagcggac cgatggccgc cgtcccgatg gcggcgcaga agcgctgcgc
     3121 cgcaaggacc accgggcgat cgcgcgtgtc gaggtgcgag gcgacgtggc gcatcgtgga
     3181 cggcgtcagc acctgctgca gcatctcttc ggcggcggcg cggatgcccg ggtcggtgtg
     3241 attgttcgaa tgggcccgaa gcgtctcggc cagcaccacc gccgccggga agtcgccgac
     3301 gacgagcagg acgctgaggc gctggagcgc cagcgccgcc aggtcgcgcc agtgcgcgag
     3361 gtccggctgc agccgcagca ggtcggccag cagcgcggcg tcgagcagcc ggacggcgtc
     3421 ctcgccgacc gtctcgcgcc aggccgcgat caggctcggc gaatcggcgc gatcctcgta
     3481 gagctcgacg gcccggccgg cgagacgatc gagctcggcg ttgtaggcgt ccgacacgaa
     3541 cttccgatcc gagtaggcga gcaggaccgc ttcggactgc tgccaggcct tggcgagcga
     3601 aggactgggg gcgtcctcgg aggtggcagc aaggttccgc gccagggtga caatggcggc
     3661 ccggcggtcc gggtccggcg cgaggccgca gaaggcatcc gcgagcgcgg gcgacgtgcc
     3721 ccggccccct cgcacttccg ccgcgaggag cgcggcgatg gccccgtcgc tgacgcgacg
     3781 ggtcagccct tcgacgaaac gcgcgagccc ggctcgattc gatccgcgac tgacgccgat
     3841 gaccggaccc agggtgtcga ggggcagccg ggccatggcg gcggccaggg cgtccagcac
     3901 cgggtcgatg ccctcggggt cggacgccgc ggcgaactgc gccaccgcct ggagcaggct
     3961 gaccaccact gccggcgtct ggccccccgc gccggcaggc gccagacgcg actcgatggc
     4021 ctcgaccacg ccggcaatcc gcgacgaatc gtcgagcacg ccaaacagca tctccacgat
     4081 ggcgtcgtcg agcgaaaagg cgagaccttc gagacactgg gcggcgatgg cttcccaggt
     4141 cgcccgctcg ccgccgctcc gcacgcggag gagttcgtgg tagttcacgg cgcgaagttc
     4201 aatgcgcgtt tcgccttctg atgcccagag cctgctgagg ccgccccgca gtcgcgcctg
     4261 ctcaggctgc agcgtcagca gcgcgagaaa caggcgccag acctccggcg tggtgtgcgg
     4321 atggactgag aacagcccga cctggtggcg gtgcaggagg cttgcgatct cggcggcggc
     4381 cgggtccggc ttgagcatcc ggcggccgtc caccatgagc gcgtcgggca ggacggccag
     4441 ggacagcgcc gacccgcccg cggcctcctc cgcggcggca gtcactgcct cgagcgcggc
     4501 ggccaacgcc ggatggtcgg acgggtacaa ggcaaccgcc cgggcggcgg ctcggcacgc
     4561 ccgtgcgaag gcggcgatgc ggttcgccag atcgggcggc atcggcccgg cgcccgctgc
     4621 cggttcactc agcgcctcgc tcacagcacc gcctcgaccc gcgccccgga gcagacggct
     4681 cgcgccgacg gccgcgcgcc gtcgcacggc acctcggccc gaaggcgtcg cccggtttgc
     4741 gctcgtcgct ctggcacagt cccaccctgc cgcgcgaggc ggctctctag ggaatatcgg
     4801 caagtccagc cgcgactttc gctatactgc tgcccttgat gcacgccgtc gaaatcatcc
     4861 ggaaaaagcg ggatgggctg gccctggagc gagacgacgt ggctcgattc gtctcgggcg
     4921 ccaccgacgg g
//
LOCUS       DAOSIF010000202         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15299_length_4920_cov_4.600206, whole genome shotgun sequence.
ACCESSION   DAOSIF010000202 DAOSIF010000000
VERSION     DAOSIF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15299_length_4920_cov_4.600206"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLT35_07805"
     CDS             <1..297
                     /locus_tag="PLT35_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOG29169.1"
                     /translation="PMGGVSFDHTAHAKAAGGDAKCVTCHHASKPEKPSKSPTQKCQE
                     CHTKAVAAPMKTNSKAAFHDAMAKKGLCVDCHAKEIAAGKKIPSKCNECHTIKG"
     gene            complement(542..1246)
                     /locus_tag="PLT35_07810"
     CDS             complement(542..1246)
                     /locus_tag="PLT35_07810"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HOG29170.1"
                     /translation="MPRDPTPRLRAQSPRFGGLDTPEGYLFELTGGSLCLDLANTLDE
                     RLRERPRELLRDYKSACDWGLQAGALSPPEYRALVRRSKALPAEAEKALHRLRKGRET
                     VFETFSAIARGKAVPAAALAALDVLIRAAVSRRGLSGGTGPLAWEWRRSDTPDLDRPL
                     WAAVLSAMDLVTSDTVRRVRQCEGAGCAWLFIDSSKNGSRRWCDMSVCGNRAKARRFR
                     ARSGALEALELTGPLQ"
     gene            complement(1246..2196)
                     /locus_tag="PLT35_07815"
     CDS             complement(1246..2196)
                     /locus_tag="PLT35_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG29171.1"
                     /translation="MEAELRGWARRRGFAVAWGPAGVLPLARRVVQWQRRTGKIDERF
                     ASRHLSFARGRPPRGWQALVVAMPRPAHSVGFVTSGRRIQALFPPTYERYLRTFEDVR
                     CDLAEGPLKGCELAVAAAPLKTAASLLGLVRYGRNNLTYAAGMGSYLQLLGYVTNARL
                     RVPPDWRPCVPRLLDECDGCRICEARCPTGAISSERVLLRAELCLTLANETPGPWPSH
                     VPSGVHHCLIGCLSCQRDCPANPELPVVESGVEFSEAETRALLDGRSRTGCVREAIGR
                     KLDLLGQPCQEEVIGRNLRALSADRVDPAAGTMCGACGSA"
     gene            2387..2956
                     /locus_tag="PLT35_07820"
     CDS             2387..2956
                     /locus_tag="PLT35_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29172.1"
                     /translation="MLVEFLRKRGRRYLLYLGVAVPAAMCHNLLHEAVHYIGAIVFGE
                     GVTAFRFLTNGWGTSQVVFSMPVEQRVGGHWLVVAWAPSIVTTLLGYGLYAARSVAGR
                     HPVSRYAWLYAGFFFLLLDPFYMSLLSLAVGGDVEAAAAVGLPVWPVQAVAAAVLLFN
                     VRLFTRWQKEVQEARVGTEEMQPGAHGGG"
     gene            3537..4283
                     /locus_tag="PLT35_07825"
     CDS             3537..4283
                     /locus_tag="PLT35_07825"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG29173.1"
                     /translation="MDAEAYDRWYETPYGRWAGQREVALLLEELAPRPGETVLDVGCG
                     TGFFTRALAAATGGPVTGVDLDTDWLAYARRRSAAGVAYEAADARSLPYGPASFDLVV
                     SITALCFIEEERAAVGELLRVARRRVAIGLLNRRSLLWLQKGRRGGRNGYRGARWHTV
                     REAAGLLAGLHAGRTRVRTALHLPGGGRLARAMERLSPRWLHTGAFILLVADVPGAAA
                     ASSGGDEQGTAPRAVPGVKRGHGPRPAAFR"
     gene            complement(4595..>4920)
                     /locus_tag="PLT35_07830"
     CDS             complement(4595..>4920)
                     /locus_tag="PLT35_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29174.1"
                     /translation="QADRLMAEAIRTVAALYADGILKKPSANASPYPDLLEFHDAPTQ
                     IEQKLFVMFLEHRMRAFQGTFHASPDYALWYGWSELKRDVTQIADMAQELRRNARVST
                     AAGNR"
BASE COUNT          716 a         1741 c         1738 g          725 t
ORIGIN      
        1 ccgatgggcg gcgtgagctt cgatcacacg gcccatgcca aggccgccgg cggcgacgcg
       61 aagtgcgtga cgtgccatca tgcgtccaag cccgagaagc cgtcgaagtc gccgactcag
      121 aagtgccagg aatgccacac caaggccgtg gccgcgccga tgaagaccaa ttcgaaggcg
      181 gccttccacg acgcgatggc gaagaagggc ctgtgcgtcg actgccacgc caaggagatc
      241 gccgccggca agaagatccc gtcgaagtgc aacgagtgcc acacgatcaa gggctaggcg
      301 cgtagcgatc cgacgcccct cgcgcgatgc ggccgggacg agcgtcccgg ccgccccgtt
      361 cgcgcacacg ccaggcccct ggcccgccgc acgagcagca gccggccgcc atcctgcccg
      421 gccagaaggc gccccgcgac tcttcccacg ttcttgggaa accgcacatg cgaagggccc
      481 cgtcgacggg gccctcagcc gtctggatgg cacgtcgtgc ggcctgaagg aagcgattcc
      541 ttcattgcag gggtccggtc aactccaacg cctccaacgc gccggacctg gcacggaaac
      601 gacgggcctt ggcgcgattg ccgcagaccg acatgtcgca ccagcgccgg gacccgttct
      661 tgctcgagtc gatgaacagc caggcgcacc ccgccccctc gcactgccgc acgcggcgca
      721 cggtgtccga ggtcacgagg tccatcgccg acaggacggc ggcccagagc gggcggtcga
      781 gatccggggt gtcgctgcgg cgccactccc acgccaacgg accggtcccg ccgctcagtc
      841 cgcgccttga gacggccgct cggatcaaga catcgagcgc cgccagtgcg gcggctggca
      901 cggctttgcc gcgggcgatc gcagagaagg tctcgaaaac cgtctcgcgt cccttccgga
      961 ggcggtgaag cgccttctcc gcctccgccg ggagggcctt cgatcgcctg accagcgcgc
     1021 gatactccgg cggcgagagc gcccccgcct gcaggcccca gtcgcacgcg gacttgtagt
     1081 cccgcagtaa ctcccgaggc cgctctcgaa ggcgctcgtc gagcgtgttg gcgaggtcca
     1141 ggcagaggct gccccccgtc agctcaaaga gataaccctc tggcgtatcg aggccgccga
     1201 aacggggcga ctgcgcacgg agtcggggcg tggggtccct aggcatcagg cagatccgca
     1261 ggcgccgcac attgtacctg ctgcaggatc gacacgatcc gctgacaacg cccgcaggtt
     1321 gcggccgatc acctcttcct ggcagggctg gccaagcaga tccagcttgc gcccgatcgc
     1381 ctcccggacg cagccggttc ggcttcgtcc gtcaaggagc gcgcgcgtct cggcttcgct
     1441 gaactcgacg ccgctctcca ccacgggcaa ctcggggttg gccgggcagt ctcgctgaca
     1501 ggacaggcag ccgatgaggc agtggtgcac ccctgaaggc acgtgcgacg gccacgggcc
     1561 cggcgtttcg ttggcgaggg tcaggcacag ctccgctcgg agaaggacgc gttccgagct
     1621 gattgcgccg gtcgggcacc tggcctcgca gatgcggcac ccgtcgcatt cgtcaaggag
     1681 ccgggggacg caaggccgcc agtcgggagg cacgcgcagc cgcgcgttcg tgacgtagcc
     1741 gagcaactgc aggtaactgc ccatcccggc ggcgtacgtc aggttgttcc ggccgtaccg
     1801 gacgaggccg agcagcgacg ctgccgtctt gagcggggcc gccgcgacgg ccagctcgca
     1861 ccccttgagc ggcccctcgg cgaggtcgca ccgaacgtcc tcgaaggtgc gcaggtaccg
     1921 ttcgtaggtc ggcgggaaga gggcctggat gcggcggccg gacgtcacga agcccaccga
     1981 gtgcgcgggg cgaggcatcg ccacgaccag ggcctgccac ccgcgcggcg gacggccgcg
     2041 ggcgaatgac agatgcctgg aggcgaatcg ctcgtcgatc ttccccgttc gcctctgcca
     2101 ctgcacgacc cgcctcgcca gcggcagcac gccggccggc ccccatgcca cggcgaaccc
     2161 cctgcgtcgc gcccagcccc gcagctcggc ttccaccgtt cacctcctcg cttgacggtc
     2221 gagagtaact atcataacgt gtatagctag ttactcgctc ccggagtgtc ccggaggcgc
     2281 accgaggtcg gcctggatct tcggctgccg ccgccaggcc ccttgccgga cacccgttcg
     2341 ctcgagcgcg cttgcccggg ttcctgggga aaggcaggtg atttccttgc tggtagagtt
     2401 tttgcggaag cggggcagga ggtatctcct gtaccttggc gtggccgtgc cggccgccat
     2461 gtgccacaac ctgctgcacg aagccgtgca ctacatcggc gcgatcgtgt tcggcgaagg
     2521 cgtgacggcc ttccggttcc tgaccaatgg ctggggaacc agccaggtgg tgttcagcat
     2581 gcccgtcgag cagcgcgtgg gcggacattg gctggtggtg gcgtgggctc ccagcatcgt
     2641 aacgaccctg ctcgggtacg gcctctacgc ggcccgcagc gtggctgggc gacacccggt
     2701 gtcccggtac gcgtggctgt acgccgggtt cttcttcctg ctcctcgatc ccttctacat
     2761 gtcgctgctt tcgctggctg tcgggggcga cgtggaagca gccgccgcgg tcggcctgcc
     2821 ggtgtggcca gtgcaggctg tagcagcggc ggttctgctg ttcaacgtga gactgttcac
     2881 ccgctggcag aaggaggtgc aagaggctcg ggtcgggacg gaagagatgc agccaggcgc
     2941 gcatggcggc ggttgacaag cgcacaggac gattgcgaac gcgagacagg ctggggtgcg
     3001 gcttccccgc gcccgctgct cgagcctctg ggctacgtgc ttccggcggc ggacgaagcg
     3061 cgcgacgatg agctggccac cgtggtcgga ctcacgccaa ccgccctccg atgaagccgg
     3121 tacggttcct cgctccctcg caggcgggac gcccgcacgg actgctgaca ggactcgtca
     3181 gaccgaactc accgaggccc gcccgccggc acgacgctgg tgccctacag ccgacaggcc
     3241 ggacgcaggg cttgccggcc atcgcgcttc gcgttacaac gttcgcacct gttcccgaag
     3301 gcctgcgatg gcgtgctcgg ccctctcgtc tcggcgtgcg gcgcgggccg gcgcgtggat
     3361 ccgcgtcgcg gcatctgcgc ccgcgcgggc gtccggcccc gccagggtga tcgaggggct
     3421 cgatcgccgt cctttgacgg gcagggtggc cgaaggccgg gcctccagtc gccagggccc
     3481 ctcttttctt cgatccgttc gccgcgggac ggcgacctgc cggaagggcg agcgctgtgg
     3541 acgccgaagc ctacgatcgg tggtatgaga cgccgtacgg ccggtgggcc ggccagcgcg
     3601 aggtggcgct gctcctcgag gaactcgccc cccgcccggg ggagacggtg ctcgatgttg
     3661 gctgcggcac gggcttcttc acgcgcgccc tggccgccgc aaccggaggg cctgtcaccg
     3721 gcgtcgacct ggacacggac tggctggcgt acgcccggcg ccggagtgcg gccggtgttg
     3781 cctacgaagc cgccgacgcg cggagccttc cgtacgggcc cgcgagcttc gatctggtcg
     3841 tgtcgatcac cgcgctctgc ttcatcgagg aagagcgcgc cgccgtcggc gagctgctcc
     3901 gcgtcgcgcg ccgcagggtc gccatcggcc tgctgaaccg ccgcagcctc ctgtggctcc
     3961 agaagggccg gcgcggcggc cgcaacggct accgcggcgc tcgctggcac acggtgcgcg
     4021 aggccgcggg cctgctggcc gggttgcacg ccgggcgcac gcgggtgcgc acggcgctgc
     4081 acctgcccgg cggcgggcga ctcgcgcggg ccatggaacg cctctcgccg cgctggctgc
     4141 acaccggcgc gttcatcctg ctggtggcag acgtccctgg agctgcggca gcatcctccg
     4201 gcggcgacga gcagggcacg gcgccgcgtg ctgtcccggg tgtcaagcgg gggcacgggc
     4261 cgcgtcccgc agcatttcgt taacggggga ggtccgctcg tgtcggtgtc gggatcgcgc
     4321 tgccgcgccg acgctcgacg ttgcctcgct cgaaggcaat ctcgacgcgg caagcgccgc
     4381 gggactgatc cggctggaga cgagcctcgg ccccgacgtc agcgccgcac cgcccgcgct
     4441 gctggatcgc cggatggatc gcggccgaga cgaacggatc gcgtgaccgg gctgaacctc
     4501 gccgtcccgg cacggaggtc gcgccgcggc gccggcggtc cactccagcc ggcgcccgcc
     4561 cggtcgcggc gatgcggcgc aggcgcgcgc gaagctaccg gttgccggcg gcagtcgata
     4621 cccgcgcgtt ccggcgcagc tcctgcgcca tgtcggcgat ctgggtgacg tcgcgcttca
     4681 gctcgctcca gccgtaccac agcgcgtagt cagggcttgc gtggaacgtg ccctggaacg
     4741 cgcgcatccg gtgctccagg aacatgacga acagcttctg ctcgatctgc gtcggcgcgt
     4801 cgtggaactc gagcaggtcc ggatacggcg aggcgttcgc cgacggcttc ttcaggatgc
     4861 cgtccgcgta gagcgcggcg acggtgcgga tggcttcggc catgaggcga tcggcctgcc
//
LOCUS       DAOSIF010000203         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15301_length_4920_cov_3.897225, whole genome shotgun sequence.
ACCESSION   DAOSIF010000203 DAOSIF010000000
VERSION     DAOSIF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15301_length_4920_cov_3.897225"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLT35_07835"
     CDS             complement(<1..744)
                     /locus_tag="PLT35_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29175.1"
                     /translation="MTGAAPSSSHFTPLDLAVVAAYFAGTMAAGFAFSRRSRSVEGFT
                     AADRSLPGWLTGLSILGTYISSITFLALPAKAYAANWNTFVFSLSIPLAAWIAVTWFL
                     PFYRHARRVSAYEHLEERFGPWARVYTSLCYLLTQLARMGTVMYLMALPMSVLLGWDV
                     RAIILITGASVTLYALVGGIVAVIYTDAFQTLVLMAGALVCAAVMVAGLPGGAGGLLR
                     LAEAHDKFSLGSFGPALTEPTFWVVLLYGL"
     gene            940..1863
                     /locus_tag="PLT35_07840"
     CDS             940..1863
                     /locus_tag="PLT35_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOG29176.1"
                     /translation="MRADRRLWIAGLVLALASTGAYLAAAPGQGGQAAAAPAAPLNII
                     VFGAHPDDCDIRAGGTAAKWVAAGHRVRFVSMTNGDAGHQSEGGGALGARRRAEAQEA
                     GRRIGVEYIVLDNHDGELMPDLRVREQVIRLIRQWKADLVLGPRPNDYHPDHRYTGIL
                     LQDAAFMVTVPNVAPDTPSLRENPVFMYVEDGFQKPNPFTPDVAVSIDDTFQKKIDML
                     DAHVSQVYEWLPWHAGRLDQVPKDPAARRQMLAKQREGRISPATREALVKYYGPEAGA
                     RVQHAEAFEICEYGRRPSQAEILRLFPFLPR"
     gene            1871..3274
                     /locus_tag="PLT35_07845"
     CDS             1871..3274
                     /locus_tag="PLT35_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG29177.1"
                     /translation="MSDIPAGAPASSRAPTLVRRFGLLQATALNMSNMIGIGPFITIP
                     LLMTALGGPQALVGWLAALVIVVADGLVWSELGAAMPGSGGSYRYLRDGFGAERWGRL
                     VAFLFIWQFVLSGPLEIASGYIGFSRYLRYVWPDLSQGGAVVVAMSVGVLNIALLYRQ
                     IGSIGKLTVSLWVGTLLTTLAVIVTGALHFDPALAFDVPPGGLSLSVGFLVGLGAASR
                     VGVYDYLGYYNVAYIGDEVKDPGRVIPRSILISVVAVAFIYIGINLAILGVVPWREFV
                     PAAAHPESDFLVSSFMERVYGSRVATLFTALVLWTAFGSVFALLLGYSRIPYAAAADG
                     TFFRVFARLHPVKRIPHVSLLLIGIIAIAASALSLGMVIDALITTRILVQFIGQIVAL
                     TLLRRNAPDMVRPFRVWLYPLPNLVALFGWIFLFATSGWQVIAFGLGTLLAGVAGFFG
                     WSRWTRRWPFAAARAAI"
     gene            3294..>4920
                     /locus_tag="PLT35_07850"
     CDS             3294..>4920
                     /locus_tag="PLT35_07850"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOG29178.1"
                     /translation="MRRREAAPSVTALAFLLLAPAASLAQGEAPVRTVIAVRADAPPT
                     IDGRLDDPVWARAVPVSGFLQRDPQEGAPATEETSVRIAFDDRALYVAGDLRDREPGA
                     IVRQLSRRDAAVEADGFVLYLDPHDDKLTGAQFAVSAAGVQRDALIYNDTYLDPSWDA
                     VWESAVAVGPGGWTVELRIPLSQLRFPRAERYTWGINVQRILQRRNESDWLQLVPKNE
                     SGLASRMARLEGIAGIDPPSTLQLMPYVTGRAEFIAPSSAGTPFNDGSRGFASGGLDL
                     KYGVSSSLTLDAAFNPDFGQVEVDPAVVNLTQFETFFEERRPFFTEGASVFRNFGKSG
                     ASAQVGFFRPEPTLFYSRRIGRAPQIAAVGPYVDAPANTTILGAAKLIGRTAGGWTLG
                     ALEAVTGRERARVSDGLTTTRPEVEPLEHERERFARRRDDPGVEGMADGEIAHLAPPL
                     GEDRDRALDRLARPADHRLGGGVDVRHHDVAVEAGEHLLDLGERREDGGHPAGVADRE
                     AGHLPAAGRDRFERLGEREAAGGHERAVLAEAVS"
BASE COUNT          682 a         1753 c         1746 g          739 t
ORIGIN      
        1 gagcccgtag agcagcacga cccagaaggt cggctcggtc agcgcgggtc cgaagctgcc
       61 caggctgaac ttgtcgtgcg cctcggcgag ccggagcagc ccgccggccc cgccgggcag
      121 gccggcgacc atcacggccg cgcagaccag cgcgccggcc atgagcacca gcgtctggaa
      181 ggcgtcggtg tagatgacgg cgacgatgcc gccgacgagg gcgtagagcg tgaccgacgc
      241 gccggtgatc aggatgatgg cgcgcacgtc ccagccgagc aggacgctca tcggcagcgc
      301 catgaggtac atgaccgtgc ccatccgggc gagctgcgtc agcaggtagc acaggctcgt
      361 gtagacgcgg gcccaggggc cgaaacgctc ctcgaggtgc tcgtacgccg agacgcggcg
      421 cgcgtggcgg tagaagggca ggaaccacgt caccgcgatc cacgcggcca gggggatcga
      481 gaggctgaac acgaacgtgt tccagtttgc cgcgtacgcc ttcgcgggca gcgcgaggaa
      541 cgtgatgctg ctgatgtacg tgccgaggat cgagaggccg gtcagccagc ccggcaggct
      601 gcggtccgcc gcggtgaagc cctcgaccga gcggctcctg cgcgagaacg cgaagcccgc
      661 ggccatcgtg cccgcgaagt aggcggcgac gacggcgaga tcgagaggcg tgaagtgcga
      721 ggagctcgga gcggcgccgg tcaccgaggc agtctacgcc aaagtccgcc ggatgccgca
      781 tgcgatcgcc cgaagtgcgc cgccccgccc gcgcaggccc cggaccggag cgggcggagg
      841 cgattccagg cccgcggcgc gccgatcccg gatagaatcc agaaaccgcc cctcccggcg
      901 cgcgccgact cgcgcgcccg gctcacagga ggtcatccca tgcgcgctga tcgccgtctt
      961 tggatcgccg gtctcgtgct tgcgcttgct tcgacaggcg cctaccttgc ggccgctccc
     1021 gggcaaggcg gccaggcggc ggccgcgcct gcggccccgc tcaacatcat cgtcttcggc
     1081 gcccaccccg acgactgcga catccgggcc ggcggcacgg ccgccaagtg ggtggccgca
     1141 ggtcaccggg tccggttcgt gtcgatgacc aacggcgacg cgggccacca gtcggagggc
     1201 ggcggcgcgc tgggcgcgcg gcgaagggcc gaggcgcaag aggcgggccg ccgcatcggt
     1261 gtcgagtaca tcgttctcga caaccacgac ggcgagctga tgccggacct cagggtgcgc
     1321 gagcaggtca tccgtctcat ccgccagtgg aaggccgacc tcgtcctcgg gccccgcccg
     1381 aacgactatc atcccgacca ccgctacacg ggcatcctcc tgcaggatgc cgccttcatg
     1441 gtgaccgttc ccaacgtggc gcccgacacg ccgtccctgc gcgagaaccc ggtgttcatg
     1501 tacgtcgaag acggcttcca gaagccgaac cccttcacgc cggacgtggc ggtgtcgatc
     1561 gacgacacgt tccagaagaa gatcgacatg ctcgacgcgc acgtctcgca ggtctacgaa
     1621 tggctgccat ggcacgcggg ccggctggat caggtgccga aggatcccgc cgcgcgtcgg
     1681 cagatgctgg cgaagcagcg cgaggggcgc attagtcccg cgacgcgcga ggcgctggtc
     1741 aagtactacg ggcccgaggc gggcgccagg gtgcagcatg cggaggcgtt cgagatctgc
     1801 gagtacggcc ggcggccgag ccaggccgag attctgaggc tgttcccgtt cctgcctagg
     1861 tagccgcgtc gtgagcgaca tcccggccgg ggcgcccgcg tcctcgcggg cgcccacgct
     1921 cgtgcggcgc ttcggcctgc tccaggccac ggcgctgaac atgtcgaaca tgatcggcat
     1981 cgggcccttc atcacgatcc cgctgctgat gacggccctc ggcgggccgc aggcgctcgt
     2041 cggctggctc gcggcgctcg tcattgtcgt cgccgacggc ctggtgtgga gcgaactcgg
     2101 cgccgcgatg ccaggctcgg gcgggtcgta ccgctacctc cgggacggct tcggcgccga
     2161 gcggtggggc cggctggtcg cgttcctgtt catttggcag ttcgtcctga gcggccccct
     2221 cgagatcgcc tccggctaca tcggcttctc gcggtacctc cgctacgtgt ggccggacct
     2281 gtcgcagggc ggcgccgtcg tggtggccat gagcgtcggc gtcctcaaca tcgcgctcct
     2341 gtaccggcag atcggatcga tcggcaagct caccgtcagt ctgtgggtcg gcaccctcct
     2401 gacgaccctc gcggtcatcg tcaccggcgc cctgcacttc gatccggccc tcgccttcga
     2461 cgtgccgccg ggcgggctgt cgctgtcggt gggattcctc gtcgggctgg gggccgcctc
     2521 gcgcgtcggc gtctacgact acctgggcta ctacaacgtc gcctacatcg gcgacgaggt
     2581 gaaggacccg gggcgggtga tcccccggtc gatcctgatc agcgtggtgg cggtggcgtt
     2641 catctacatc gggatcaacc tggcgatcct gggcgtggtg ccgtggcgcg agttcgtgcc
     2701 ggccgccgcg cacccggagt ccgacttcct tgtctcgagc ttcatggagc gggtctacgg
     2761 gtcgcgcgtg gccaccctct tcaccgcgct cgtgctgtgg accgcgttcg gctcggtgtt
     2821 cgcgctgctg ctcggctatt cgcggatccc ctacgcggcg gccgccgacg ggacgttctt
     2881 ccgcgtgttt gcccggctgc acccggtcaa gcgcatcccg cacgtgtcgc tgctgctcat
     2941 cgggatcatc gcgattgcgg ccagcgccct gtcgctcggc atggtgattg acgcgctgat
     3001 cacgacccgg atcctggtgc agttcatcgg ccagatcgtc gcgctgacgc tgctgcggcg
     3061 gaacgcgccg gacatggtgc ggccgttccg cgtgtggctg tacccgctgc cgaacctcgt
     3121 ggccttgttc ggctggatct tcctgttcgc cacgtcgggc tggcaggtga tcgcgttcgg
     3181 cctcggcacg ctcctcgccg gcgtggcggg ctttttcgga tggtcccggt ggaccagacg
     3241 gtggcctttc gccgcagctc gagcggcgat ctgagctgaa gggagtctgc ggcatgcgcc
     3301 gtcgcgaagc ggcgccgtcc gtgacggcgc tggcgtttct gctgctcgcc cccgcggcgt
     3361 cactcgccca aggggaggcg ccggtccgga cggtgatcgc cgtgcgggcc gacgcgcccc
     3421 ccaccatcga cgggcggctc gacgacccgg tgtgggcccg ggcggtgccc gtgtcgggct
     3481 tcctgcagcg ggacccgcag gaaggcgctc ccgccaccga ggagacgagc gtgcgcatcg
     3541 cgttcgatga tcgcgcgctg tacgtggccg gcgacctgcg ggatcgcgag ccgggcgcca
     3601 tcgtgcgcca gctgtcgcgg cgtgacgcag cggtggaggc cgacggcttc gtcctgtacc
     3661 tggatcccca cgacgacaag ctgacgggcg cgcagttcgc ggtgtcggcc gcgggcgtgc
     3721 agcgcgacgc cctgatctac aacgacacct acctcgaccc ctcctgggac gccgtctggg
     3781 agtcggccgt cgccgtcggc cccggcggct ggacggtgga actgcgcatc ccgctgtccc
     3841 agctgcgctt tccccgtgcc gagcgctaca cgtggggcat caacgtccag cggatcctgc
     3901 agcggcgcaa cgagagcgac tggctgcagc tcgtgcccaa gaacgaatcc gggctcgcct
     3961 cgcgcatggc gcgcctcgaa ggcatcgccg gcatcgaccc tccctcgacc ctgcagctga
     4021 tgccctacgt gacgggccgc gccgagttca tcgcgccatc gtccgcgggc acgccgttca
     4081 acgacggctc gcgcgggttt gcctccgggg gcctcgacct caagtacggc gtctcgagca
     4141 gcctgacgct cgacgccgcg ttcaacccgg acttcggcca ggtcgaggtc gacccggccg
     4201 tcgtcaacct gacgcagttc gagacgttct tcgaggagcg gcggccgttc ttcaccgagg
     4261 gcgccagcgt gttccgcaac ttcggcaaga gcggcgccag cgcgcaggtg gggttctttc
     4321 gccccgagcc gacgctgttc tacagccggc gcatcggcag ggcgccgcag atcgcggccg
     4381 tcggccccta cgtggacgcg cccgccaaca ccaccatcct cggcgcggcc aagctcatcg
     4441 gccgcacggc cggcgggtgg accctcggcg ccctcgaggc cgtcaccggc cgcgagcgcg
     4501 cccgcgtctc cgacggcctg acgaccacgc ggccggaggt cgagccgctg gagcatgagc
     4561 gcgagcgatt cgctcgccgg cgcgacgatc cgggtgtgga aggcatggct gacggggaga
     4621 tagcgcacct cgcgcccccg ctcggagaag accgcgaccg cgcgctcgac cgcctcgctc
     4681 gccccgccga tcaccgcctg ggcggtggag ttgatgttcg ccaccacgac gtagccgtcg
     4741 aggccggcga gcacctcctc gatctcggcg agcggcgcga agacggcggc catccggccg
     4801 gggtcgccga ccgagaggcg ggtcatctcc cggccgcggg ccgagaccgc ttcgagcgcc
     4861 tcggcgaacg agaggcagcc ggcggccacg agcgcgccgt actcgccgag gctgtgtccc
//
LOCUS       DAOSIF010000204         4876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15475_length_4876_cov_4.455507, whole genome shotgun sequence.
ACCESSION   DAOSIF010000204 DAOSIF010000000
VERSION     DAOSIF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15475_length_4876_cov_4.455507"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLT35_07855"
     CDS             <1..335
                     /locus_tag="PLT35_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOG29179.1"
                     /translation="VFTTIEALTGAPVNGVRIIGGGCQNAYLNQATSTATGKPVLAGP
                     VEATAMGNAIVQAIAAGRFASLAEARAHVARHVSPSSFEPRPTAASERAALRYAAIEA
                     RFTEGVRP"
     gene            332..1594
                     /locus_tag="PLT35_07860"
     CDS             332..1594
                     /locus_tag="PLT35_07860"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF002203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOG29180.1"
                     /translation="MSANVQQAYRLAAERYREMGVDAGAALAKLKTVPISLHCWQGDD
                     VKGFEDFGGELGGGLAATGNYPGAARTPDQLRADAAKALSLIPGTHRFNLHAFYGEFK
                     GKKVDRDEILPEHFAGWIDWARSLGLGLDFNPTYFSHPKAADNFTLSHADRGIRDFWI
                     RHGIACRRIAEAMGRAVGKTCITNFWIPDGMKDTPVDRRGPRERLIQALDEILAAPVD
                     QSCTRDSVEGKLFAIGIESYTVGSHEFYQSYSLARRTLFTLDTGHYHPTETITDKISA
                     ILSFIPEILLHVSRGVRWDSDHVVTLGDDLGAIAQELVRGDYLGRTHIGLDYFDASIN
                     RVAAWVIGVRNMLKALLQAMLEPIDRLREAERSGDYTARLALLEEAKTLPFGAVWDYY
                     CETAGVPVGEAWLSEVQAYERDVLARRG"
     gene            1611..2681
                     /locus_tag="PLT35_07865"
     CDS             1611..2681
                     /locus_tag="PLT35_07865"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOG29181.1"
                     /translation="MTPAEAAANPALGVLIFAIGGLAGAVFYLPFAKVRSWAWESYWL
                     VYAVTGLLVVPVALALATSPNLFPVLRAAPAGELAYCFFCGAMWGVGGLTWGLMIRYL
                     GVGLGLALGCGITSAAGTLVPRLLAGDIGRLFEPGAGIVSLVGVLVSLAGIVVVGLAG
                     MAKERELPEAEKRKSVAEFNFSKGVRAALFSGLMSSAMGWGLVGGPTIQRLAETTAPA
                     TSRTWSGMPVLVVVLFGGLLVNVAWCLRLNAKNKTTGDYRKAGAPLGANFALAALAGA
                     IWCSQFICFKTGEPAMGQTAYVGWALLMACQILFSSLLGVFVLGEWRQTGRRTRALLA
                     AGLSLLILTAVLSGYSGYLALA"
     gene            complement(2759..4102)
                     /locus_tag="PLT35_07870"
     CDS             complement(2759..4102)
                     /locus_tag="PLT35_07870"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG29182.1"
                     /translation="MNVRNTRREFLGFAAAAAASAGLFTRTAFGRSLADKPALLGGSP
                     VHLGGWPQWPEWRQAWEPSILEVLRSGKWYRGSGDKVQQFEAAYAKLLGAKRCLATAS
                     GTTALLVGMHVLDVDAGDEVIVSPFTFIASYNAVLMLKALPVLADTDPATLTLDPASI
                     ESRITERTRAIMPVHIYGMPCDLDPVLAIAKRHRIAVIEDACQAWLAEYKGRMCGTIG
                     DLGCFSFQNSKHLPSGEGGAITGNSDSLVDRAASFHDCGRSYGTFTGARPNFTRGGNF
                     RMQHVQAAMLLQQFDKLVQETARRRENADRLTAALRDIPGIQTVRLPGDSRAVWHLYP
                     FRYDAERFQGLSREKFLRAMRAEGIPCSTGYQEQYFDGLLDEAIGSRGFKRLFTAQRL
                     KQYRDSFRDLPGNRVVCATTVAIPQNLLLAEPRHVDHIADAIRKVQAHAAELAKA"
     gene            complement(4317..>4876)
                     /locus_tag="PLT35_07875"
     CDS             complement(4317..>4876)
                     /locus_tag="PLT35_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG29183.1"
                     /translation="PGYYLSWGGQFENQQQAMRRLAVITPVVAGLIFLLLLFTFDSVR
                     LAALVLANLPFAMVGGVFALFLSGLYLSVPASVGFIVLFGVAVLNGVVLVSHISDLRD
                     AGATVADAVRIGCEDRLRPVLMTASISILSLIPMVLATGPGSEVQRPLAVVVIGGLAT
                     STALTLLVLPTLYQWLAPGRRTARS"
BASE COUNT          706 a         1666 c         1778 g          726 t
ORIGIN      
        1 ccgtgttcac gacgatcgag gcgctgacgg gcgcgccggt caacggcgtg cgcatcatcg
       61 gcggcggatg ccagaacgcg tatctcaatc aggcgacatc gaccgcgacg ggcaagcccg
      121 tgctcgcggg cccggtcgag gcgacggcca tgggaaacgc gatcgtgcag gcgatcgccg
      181 ccggccgctt cgcctccctg gccgaggccc gagcccacgt ggcgcggcac gtgtcgccat
      241 cgtcgttcga gccgaggccc accgccgcct cggaacgcgc ggccctccgc tatgcggcca
      301 tcgaagcgcg gttcacggaa ggagtccggc catgagcgcg aatgtgcagc aggcataccg
      361 gctggcggcc gagcggtacc gggagatggg cgtcgacgcc ggggccgcgc tggcgaagct
      421 gaagacggtt ccgatctcgc tgcactgctg gcagggggac gacgtcaagg gcttcgagga
      481 cttcggcggc gagctcgggg gcggcctggc ggccaccggc aactaccccg gcgcggcccg
      541 gacgcccgac cagctgcgcg ccgacgcggc gaaggccctg tcgctgattc ccgggacgca
      601 ccggttcaac ctgcacgcgt tctacgggga attcaagggg aagaaggtcg atcgcgacga
      661 gatcctgccg gagcacttcg ccggctggat cgactgggcc agatcgctcg gcctcggcct
      721 cgacttcaat ccgacgtact tctcccaccc gaaggccgcg gacaacttca cgctgtcgca
      781 cgcggaccgc gggatccgcg acttctggat ccggcacggg atcgcctgcc ggaggatcgc
      841 cgaggcgatg ggaagggccg tcggcaagac gtgcatcacc aacttctgga tcccggacgg
      901 catgaaggac acgccggtcg atcggcgggg cccgcgcgag cggctgatcc aggccctcga
      961 cgagattctc gcggcgcccg tggaccagtc gtgcacgcgg gactcggtcg aaggcaagct
     1021 ctttgccatc ggcatcgaga gctacaccgt cggctcgcac gagttctacc agtcgtactc
     1081 cctcgcgcgg aggacgctct tcacgctcga caccgggcac taccatccga ccgagacgat
     1141 caccgacaag atcagcgcca tcctctcgtt catccccgag atcctgctgc acgtcagccg
     1201 cggcgtgcgc tgggacagcg accacgtggt cacgctcggc gacgacctcg gggccatcgc
     1261 ccaggagctc gtgcgaggcg actacctcgg ccggacgcac atcggcctcg actacttcga
     1321 cgcgagcatc aaccgcgtgg cggcgtgggt catcggcgtg cggaacatgc tgaaggcgct
     1381 gctgcaggcc atgctggagc cgatcgatcg gctccgcgaa gccgagcgct cgggcgacta
     1441 cacggcgcgc ctggcgctgc tcgaggaggc gaagacgctg ccgttcggcg cggtgtggga
     1501 ctactactgc gagaccgccg gcgtgccggt tggcgaggcg tggctgagcg aggtgcaggc
     1561 gtacgagcgc gacgtgctcg cgcggcgcgg gtgaagggaa gggctgagcg atgactccag
     1621 ccgaagctgc cgccaacccc gccctgggcg tcctgatctt cgcgatcggg ggcctcgccg
     1681 gcgcggtgtt ctacctgccg ttcgcgaagg tcagaagctg ggcctgggag agctactggc
     1741 tcgtctacgc cgtgacgggc ctgctcgtcg tgccggtcgc gctggcgctg gcgacgtcgc
     1801 cgaacctctt cccggtgctg cgggcggcgc cggcagggga gctggcctac tgcttcttct
     1861 gcggcgcgat gtggggggtg ggcggcctca cctggggcct catgatccgc tacctcggcg
     1921 tgggcctcgg cctcgcgctg ggctgcggca tcacgtccgc ggccgggacg ctcgtcccgc
     1981 ggctgctcgc gggcgacatc ggccgcctct tcgagcctgg cgccggcatc gtctcgctcg
     2041 tcggcgtcct cgtctccctc gccggcatcg tcgtggtggg gctggccggg atggcgaagg
     2101 agcgggagct gccggaggcg gagaagcgga aatcggtggc cgagttcaac ttctccaagg
     2161 gcgtcagggc ggcgctgttc tccgggctga tgagctcggc gatgggatgg ggcctcgtcg
     2221 gcggcccgac catccagagg ctggcggaga ccacggcgcc ggccacgtcg cggacgtgga
     2281 gcggcatgcc cgtcctggtc gtcgtgctgt tcggcggtct cctcgtgaac gtcgcctggt
     2341 gcctgcgcct gaatgcgaag aacaagacga ccggcgacta ccggaaggcc ggcgcgcctc
     2401 tcggggccaa cttcgccctc gcggccctgg ccggcgcgat ctggtgctcg cagttcatct
     2461 gcttcaagac gggcgagccg gcgatgggcc agacggccta cgtgggctgg gcgctcctga
     2521 tggcgtgcca gatcctcttc agttcgctgc tcggggtgtt cgtcctgggc gagtggaggc
     2581 aaacggggag gcggacgcgc gcgctgctgg ctgcgggcct gtcgctcctg atcctgaccg
     2641 ccgttctgtc gggctacagc ggctacctgg cgctggcgta gccggcagcc cggccgccgc
     2701 gctgagggcg ccgcttcctg ctccccaatc cggccggccc gcctggccgg gggccgggct
     2761 acgccttcgc cagctccgcg gcgtgggcct gcaccttccg gatcgcgtcg gcgatgtgat
     2821 ccacgtggcg gggctcggcg agcagcaggt tctgcgggat cgccaccgtc gtggcgcaga
     2881 ccacgcggtt gcccgggagg tcgcggaagc tgtcgcggta ctgcttcagc cgctgagcgg
     2941 tgaagagccg cttgaacccg cgcgagccga tcgcttcgtc caggagcccg tcgaagtact
     3001 gctcctgata gccggttgaa cagggaatcc cctcggcgcg catcgccctg aggaacttct
     3061 cgcgtgagag gccctggaac cgctccgcgt cgtagcggaa cgggtacagg tgccagaccg
     3121 cgcggctgtc cccgggcagg cgcacggtct gaatgccggg gatgtccctg agcgcggcgg
     3181 tcaggcgatc ggcgttctcc cgccggcggg ccgtttcctg taccagcttg tcgaattgct
     3241 ggaggagcat ggcggcctgg acgtgctgca tccggaagtt gccgcccctc gtgaagttgg
     3301 ggcgcgcgcc cgtgaaggtc ccgtaggagc ggccgcagtc gtggaacgac gcggcccgat
     3361 cgacgaggct gtcgctgttg ccggtgatgg cgccgccctc gcccgacggg aggtgcttcg
     3421 agttctggaa gctgaagcac ccgaggtcgc cgatcgtgcc gcacatgcgc cccttgtact
     3481 cggccagcca cgcctggcac gcgtcctcga tcactgcaat ccggtgccgc ttcgcaatgg
     3541 cgaggaccgg atcgaggtcg cagggcatgc cgtagatgtg cacgggcatg atcgcgcggg
     3601 ttcgctcggt gatccggctc tcgatggatg ccgggtccag cgtcagcgtt gccggatcgg
     3661 tgtcggcgag caccggcagg gccttgagca tcaggaccgc gttgtagctg gcgatgaacg
     3721 tgaacggcga cacgatgacc tcgtcgccgg cgtcgacgtc gagcacgtgc atgccgacga
     3781 ggagggccgt cgtaccgctg gcggtggcca ggcagcgctt cgcgccgagc agcttcgcgt
     3841 acgcggcttc gaactgctgc accttgtcgc ccgagcctcg gtaccacttg ccgctgcgca
     3901 gcacctccag gatggacggc tcccaggcct gccgccactc cggccactgc ggccagcctc
     3961 ccaggtgcac gggcgagcct ccgagcaggg ccggcttgtc cgcaagagag cggccgaagg
     4021 cggttcgcgt gaacaggccc gcgctcgcgg cggcggccgc ggcgaagccg aggaactccc
     4081 ggcgggtgtt tctgacgttc atcgccccct cacgccgccc atcttacacc cgggcgccgg
     4141 gattggcgcg ccgccgagcg cctagatccg cctgccggac gggccggcgt gcgcatcccc
     4201 gtcctgctcc cggctccgcc ggatggcgtg ccgggcctcg cgaggcggcg tgccggcaga
     4261 cggcgctgcc gtcccggtcc cggctcacgc ctcgcgccgc agccggtgcg gtcgtgtcag
     4321 gaccgcgccg tcctccgtcc cggagcgagc cattgataca gcgtcggcag cacgagcagc
     4381 gtgagggcgg tcgaggtggc aagccctccg atcacgacca cggccagggg gcgctgcacc
     4441 tccgagcccg ggccggtcgc cagcaccatc ggaatcaggc tcaggatcga gatgctggcg
     4501 gtcatgagga ccgggcggag ccggtcttcg cagccgatgc gcaccgcgtc ggcgacggtc
     4561 gctccggcgt cgcgaaggtc ggagatgtgc gagacgagca ccacgccgtt cagcaccgcg
     4621 acgccgaaca ggacgatgaa gccgacagag gccgggacgg acaggtacag cccggagagg
     4681 aagagcgcga acacgccgcc caccatcgcg aagggcaggt tggccaggac gagcgccgcc
     4741 agccggaccg agtcgaaggt gaacaggagc agcaggaaga tgaggcccgc cacgacgggc
     4801 gtgatgaccg cgaggcgccg catggcctgc tgctggttct cgaactggcc tccccagctc
     4861 aggtagtagc cgggcg
//
LOCUS       DAOSIF010000205         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15502_length_4868_cov_3.831290, whole genome shotgun sequence.
ACCESSION   DAOSIF010000205 DAOSIF010000000
VERSION     DAOSIF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15502_length_4868_cov_3.831290"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /gene="pepF"
                     /locus_tag="PLT35_07880"
     CDS             complement(<1..1760)
                     /gene="pepF"
                     /locus_tag="PLT35_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOG29184.1"
                     /translation="MKPQTRERAEIPDRYKWDLSAIYPSWNAWDEAYARLGALVSAFA
                     SRRGTLASGPDALLEALRAADELGRLAHSVYYFPALFHDQDQRDNAVGARRQMAQIQV
                     ARWQEAASWFNPELLEIPLARVREWMAGRADLAVYQFAVENLYRLQTHVLDAKGERLM
                     SLVSRFDSTAAEAYEALSTADAAFPTIELTDGRRVTVSYGEYRSILTTSRQQADREAA
                     FRAYLGTFEASRNTYAALYNGVCQRDWAAAQARGYGSTLEAALFVHDIPPAVVETLIA
                     VTRANAASLQRYHRLRKRALGLDRYCLFDGQVPIVGLERAYDYDEARAWIEASVAPLG
                     AEYQALVERSFERASIDVYENDGKRSGAYSAPVYGVHPYILLNHNDTLDAVFTLAHEM
                     GHSIHTVLAHANQPFVYSDYTIFVAEVPSTLAEALLLDHLLERTADSRERVVLLEHAI
                     ESIAGTFFSQVLYADFELRAHRLVEANQAITADVLGDIWEGLLADYYGGTLDAEPLAR
                     LTWARVPHFFSTPYYVYQYATCFASASRLADQILRGAEASRADAVGRYLDLLKAGGSG
                     HPMGLLRRAGVDLRDADTVES"
     gene            complement(1777..3801)
                     /locus_tag="PLT35_07885"
     CDS             complement(1777..3801)
                     /locus_tag="PLT35_07885"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOG29185.1"
                     /translation="MAPEDAIQSPSLEQQVEITVPDRTPPPAGGLADAAAAPAAPAGL
                     PGPAEPAGPVEVPVPAAAVEPASEGEADGEDPVRAEAPPPLDLTPAEQAERVARIGQL
                     LDGAEQVLAATDLPDARTRWNALRRDWTALAAGVALDDASAARIADIERRIEARDAAL
                     REARTRQQQTNLARLQALCDEMEQLARSERLSLRDAERALREIRAALDAPGPLPSRQE
                     QQAVVARLKAVQSLVFPRVQDLREADGWERWANAGVQESLIARLEALRDQADPAVIAK
                     QLRVVQDEWHKVRAVPREKGRELWQRYKIIEGELRLRCESFFAQLAAERVENLRKKEA
                     LCAQVESLADSTDWIRTAEAIKAIQAQWKTIGPVTPGHEKAVWERFRAACDRFFTRRK
                     TDLTERKAVWLANLKKKEAICAEAEALSESTDWERALADVKRIQAEWRLVGPVKRNRS
                     EALLARFHAACEKFFDRYAHRNDHQLAQQAAAREGLCAELEGLLTPAEEAPPGEVDPA
                     EGLAKKVLALKRQWDQSPVLPKAQAEPLVQRFDTALHAVIRSKPEAFRGTDLDPEAAR
                     RKMEQLCQLVERHAGDEKSGDQRSPAEILAAQLREALAANTIGGRADEEARWRNAADE
                     VRRAQASWRRLAPLPDEREQELEARFQRACTRFFRQLEQRRRPGSQGPRR"
     gene            complement(3965..4450)
                     /locus_tag="PLT35_07890"
     CDS             complement(3965..4450)
                     /locus_tag="PLT35_07890"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4295..4297),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG29186.1"
                     /translation="MAQTPAALLQAGDAAPAFTLPGSDGATHTLAEFAGKRAVVLAWF
                     PKAFTGGUTAECRSLRESGDLIRGYDVALFAASVDTPEDNKRFAESLGLDYPILSDPG
                     RQVAQAYGVVTPERQVAARWTFYIGLDGKILAVDRKVSAATAGQDVVARLGELGIAKK
                     Q"
     gene            complement(4547..>4868)
                     /locus_tag="PLT35_07895"
     CDS             complement(4547..>4868)
                     /locus_tag="PLT35_07895"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOG29187.1"
                     /translation="DLDHFTANLREIRRLLGEGKTRQQILDVYVARYGDRILAEPPAR
                     GFSRLFHVAPWVFLAGSVGLVWAVLRRLRPAAAAEGAVAAATAAPPDEAESERIDEEL
                     RNLD"
BASE COUNT          639 a         1749 c         1742 g          738 t
ORIGIN      
        1 gattcgacgg tgtcggcatc gcgcaggtcc actccggccc ggcgcagcag gcccatcggg
       61 tggccgctcc cgccggcttt gagcagatcc agatagcggc cgaccgcgtc ggccctcgac
      121 gcctcggcgc cgcggaggat ctggtcggcg agcctggacg ccgaggcgaa gcaggtggcg
      181 tactgataga cgtaataagg cgtcgagaag aagtgcggca cgcgcgccca cgtcaggcgg
      241 gccagtggct ccgcgtcgag cgtcccgccg tagtagtcgg cgagcaggcc ctcccagatg
      301 tcgcccagga catcggccgt gatggcctgg ttcgcctcga cgagccggtg cgcccgcagc
      361 tcgaagtcgg cgtagaggac ctggctgaag aaggtcccgg cgatgctctc gatcgcgtgc
      421 tccagcagca cgacgcgctc gcgcgaatcg gcggtccgct cgagcaggtg gtcgagcagc
      481 agcgcctcgg ccagcgtgga cggcacctcg gcgacgaaga tggtgtagtc cgagtacacg
      541 aacggctggt tcgcatgggc caggacggta tggatgctgt ggcccatctc gtgggccagg
      601 gtgaagacgg cgtccagggt gtcgttgtgg ttgagcagga tgtacgggtg cacgccgtac
      661 accggcgccg agtacgcgcc gctgcgcttg ccgtcgttct cgtagacgtc gatgctcgcg
      721 cgctcgaagc tccgctcgac cagcgcctgg tattcggcgc cgagcggcgc caccgaagcc
      781 tcgatccagg cccgggcttc gtcgtagtcg tacgcgcgct ccaggccgac gatgggcacc
      841 tggccgtcga agaggcagta gcgatccagg ccgagcgccc gcttccgcag ccggtggtag
      901 cgctgcagcg acgcggcgtt ggcgcgcgtc acggcaatca gcgtctccac gacggctgga
      961 gggatgtcgt ggacgaaaag agcggcctcg agggtggagc cgtaccccct ggcctgcgcc
     1021 gcggcccaat cccgctggca gacgccgttg tagagcgccg cgtacgtgtt gcgcgacgcc
     1081 tcgaacgtgc ccaagtacgc gcggaacgcc gcctcgcgat ccgcctgctg gcgggaggtg
     1141 gtcaggatcg atcggtactc cccgtaggac accgtgacgc ggcggccgtc ggtcagttcg
     1201 atggtcggga acgcggcgtc ggcggtggac agcgcctcgt acgcctcggc ggccgtggag
     1261 tcgaagcggc tgaccagcga catgagccgc tcgcctttcg cgtccagcac gtgggtctgc
     1321 aggcggtaca ggttctccac ggcgaactgg tagacggcga ggtcggcccg gcccgccatc
     1381 cactcgcgca cgcgcgcgag cgggatctcg agcagctcgg ggttgaacca ggaggcggcc
     1441 tcctgccacc gcgccacctg gatctgggcc atctgccgac gggcgcccac cgcattgtcg
     1501 cgctggtcct ggtcgtgaaa gagcgccggg aagtagtaga ccgagtgcgc gaggcgcccg
     1561 agttcgtcgg cggcccgcag ggcctccagc agcgcgtccg ggcccgaggc gagggtgccc
     1621 cgccgcgacg caaatgccga cacgagcgcg ccgaggcggg cgtacgcctc gtcccaggcg
     1681 ttccaggacg ggtagatggc ggacagatcc cacttgtagc ggtcgggaat ctcggcgcgc
     1741 tcccgagtct gcggcttcac gaggcgtcct ggcgggctag cgccgcggcc cctgggaccc
     1801 gggacgccgc cgctgctcca gctgccggaa gaaccgcgtg cacgcacgct ggaaccgcgc
     1861 ctcgagctcc tgctcgcgct cgtccggcag cggcgcaagc cggcgccacg atgcctgcgc
     1921 ccggcgcacc tcgtcggcgg cgttccgcca ccgagcctcc tcgtccgccc gcccgccgat
     1981 ggtgttcgcc gccagcgcct cgcgcagctg ggcggccagg atctcggcgg gagatcgctg
     2041 gtcgcctgac ttctcgtcgc cggcgtgccg ctcgacgagc tggcagagct gctccatctt
     2101 ccgccgcgcg gcttccggat cgaggtccgt gcccctgaag gcctccggtt tcgaccggat
     2161 gaccgcgtgc agcgccgtgt cgaaccgctg gacgagcggc tcggcctggg ccttgggcag
     2221 gacgggggac tggtcccact gccgcttcag tgcaagcacc ttcttcgcga ggccttcggc
     2281 cggatcgacc tcgccgggcg gggcttcctc ggccggcgtg agcaggcctt cgagctcggc
     2341 acagagccct tcgcgcgccg ctgcctgctg cgccagctgg tggtcgttgc ggtgcgcgta
     2401 gcggtcgaag aacttctcgc acgcggcatg gaaccgggcg agcagcgcct cggaccggtt
     2461 gcgcttgacg ggcccgacca ggcgccactc ggcctggatt cgcttcacgt ccgccagcgc
     2521 gcgctcccag tcggtgctct cgctgagcgc ctccgcctcg gcgcagatgg cctccttctt
     2581 cttcaggttc gcgagccaga cggccttccg ctctgtcagg tcggtcttgc ggcgcgtgaa
     2641 gaagcggtcg caggccgcgc ggaaccgctc ccacaccgcc ttttcgtgcc ccggcgtcac
     2701 gggcccgatg gttttccact gcgcctggat ggccttgatg gcctcggccg tgcggatcca
     2761 gtccgtcgag tcggcgaggg attcgacctg ggcgcagagg gcttccttct tccggaggtt
     2821 ctcgacgcgc tcggcggcga gttgcgcgaa aaaggactcg cagcgcaggc gcagctcgcc
     2881 ttcgatgatc ttgtagcgct gccacagctc gcggcccttc tcgcgcggga ccgcccgcac
     2941 cttgtgccac tcgtcctgca cgacgcgcag ctgctttgcg ataacggccg ggtccgcctg
     3001 gtcacgcagc gcctcgaggc gggcgatgag cgactcctgc acgccggcgt tggcccagcg
     3061 ttcccacccg tccgcttcgc gcaggtcctg cacgcgaggg aagaccaggg actgcacggc
     3121 cttcaggcgc gcaacgaccg cctgctgttc ctggcgggac ggcagcggcc ctggcgcgtc
     3181 gagcgcggcg cggatctcgc ggagcgcgcg ctcggcgtcc cgcaggctca ggcgctcgct
     3241 gcgcgccagt tgctccatct cgtcgcagag ggcctggagc cgcgccaggt tggtctgctg
     3301 ctggcgcgtc cgggcttcgc gaagggccgc gtccctcgcc tcgatgcgcc gctcgatgtc
     3361 cgcgatgcgc gccgccgagg cgtcgtcgag cgcgacgccc gcggcgagcg ccgtccagtc
     3421 gcggcgcagg gcgttccagc gcgtgcgcgc gtcgggcaga tcggtggcgg cgagcacctg
     3481 ttcggccccg tcgagcagct ggccgatgcg cgccacgcgc tcggcctgct cggccggcgt
     3541 cagatccagc ggcggaggcg cctcagcgcg gacgggatcc tcgccgtccg cttccccttc
     3601 cgatgccggc tcgaccgccg cggctggcac cgggacctcg acaggcccgg ccggctcggc
     3661 cggcccgggg agcccggcag gcgccgctgg ggccgcggcc gcgtcggcca ggccgcccgc
     3721 cggagggggc gtccgatcgg gcacggtgat ctcaacctgc tgttcgaggg atggcgactg
     3781 gatggcgtct tctggtgcca cgctcgtctc gcgcgcctca tcgcacgtgc cggacactac
     3841 cgagtcccga cccggcgact ggcggttcag gcccgccggg ccggcgtccc tggacgcgag
     3901 cccggcggcc gcggattggt gttcggcccg gccggattca gccgggccct cgccgccgac
     3961 ggcctcactg cttcttggcg atgccgagtt ctccaagcct ggccacgacg tcctgcccgg
     4021 ccgtcgccgc gctgaccttc cgatcgaccg cgaggatctt cccgtcgagg ccgatgtaga
     4081 acgtccaccg cgcagcgacc tgccgctcgg gagtgaccac gccgtatgcc tgcgctacct
     4141 gcctgccggg gtcgctgaga atcgggtagt ccagccccag cgactcggcg aaccgtttgt
     4201 tgtcttcggg cgtgtcgacg ctcgccgcaa acaaggccac gtcgtagccc ctgatcaggt
     4261 cgccgctctc acggagcgac cggcactcgg ccgttcatcc gccggtgaag gccttcggga
     4321 accaggcgag cacgacggcc cgcttgcccg cgaactccgc cagcgtatgg gtcgccccgt
     4381 ccgaacccgg cagcgtgaac gccggcgccg cgtcgccggc ctgcagcaac gcggccggcg
     4441 tttgggccat cgccggagcg gtcatcgaga cgcccgtcgc gagcagcagc gccagagccg
     4501 tccgcataga atactcctct gggggttgag aggatcctgc cggttcctag tcgaggttcc
     4561 gcagctcttc gtcgatccgt tccgattccg cttcgtccgg cggcgccgcc gtcgcggcgg
     4621 ccaccgcgcc ttccgccgcc gccgccggcc ggagccggcg gaggacggcc cacaccaagc
     4681 cgacgctccc agccaggaag acccacggcg cgacgtgaaa caggcggctg aagccgcggg
     4741 ccgggggctc ggcaaggatg cgatcgccgt agcgcgcgac gtaaacgtcg aggatttgct
     4801 ggcgcgtctt gccctcgccg agcaggcgcc ggatttcccg gaggttggcg gtaaagtggt
     4861 ccaggtcg
//
LOCUS       DAOSIF010000206         4833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15636_length_4833_cov_3.079950, whole genome shotgun sequence.
ACCESSION   DAOSIF010000206 DAOSIF010000000
VERSION     DAOSIF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15636_length_4833_cov_3.079950"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2203)
                     /gene="carB"
                     /locus_tag="PLT35_07900"
     CDS             complement(<1..2203)
                     /gene="carB"
                     /locus_tag="PLT35_07900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOG29188.1"
                     /translation="MPRRPDVKRVLVLGAGPIVIGQACEFDYSGSQACKALRAEGVEV
                     VLVNSNPATIMTDPELADRTYVEPLTPAFVEAVIARERPDALLPTVGGQTALNLAMAL
                     AKSGSLARYGVELVGASVAAIDVAEDRLKFRDAMREIGVGVPESGYARSLDEALSLVR
                     TTGFPSIIRPSFTLGGVGSGIAYNIEEFTEIVRRGLAMSPVHEVLIEESLIGWKEYEL
                     EVMRDAGDNFVVICSIENLDPMGVHTGDSVTVAPALTLTDKEYQAMRDAARRIIRRVG
                     VETGGSNIQFALDPRDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLALGYHLDE
                     IPNDITRLTPASFEPTIDYVVVKIPRWNFEKFPEAERTLTTQMKSVGEVMAIGRTFQD
                     AFMKGVRSLEMGRESLLFEHEWLRGEDDDGLRQRLATPTDGRLWAVFEALDRGWSATA
                     LHELTRIDPWFLAQFADLAARRRALRGKTLQDLGGADLRALKRAGFGDATIAAATGAS
                     ERAVRERRAAAGLRASYKRIDTCAAEFESLTPYLYGSYEQECEADPTPHRKAVILGSG
                     PNRIGQGIEFDYCCCHAVFGLRDEGFETVMVNCNPETVSTDYDTADRLYFEPLTLEDV
                     LAVIDKEREAGGSVACLVQFGGQTPLKLSTALHRAGVPVLGTSPDSIDLAEDRKRFAA
                     LLRDLDIPQPPSGTASSRAEARDVAVGIGFPVVVRPSYVLGGRAMAIVYDPG"
     gene            complement(2204..3334)
                     /gene="carA"
                     /locus_tag="PLT35_07905"
     CDS             complement(2204..3334)
                     /gene="carA"
                     /locus_tag="PLT35_07905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOG29189.1"
                     /translation="MRAVLALEDGTWFEGTAAGAEGTAVGEVVFNTGLTGYQEILTDP
                     SYAGQIVTMTCPEIGNYGVTPEDAESRAPQVAGFVMRRESLVASNWRAAGTLRDYLVS
                     AGTVAISDIDTRALTRRLRSAGVMRGVIGTGDARPADLVEQARRVPLMAGSDLVSGVT
                     CAAPFDWPVEGAAAGTGGYGREPLRAPARRYRVAAWDFGIKWNILRRLASYGCDIRVY
                     PASTPAAVLLSERPSGVFLSNGPGDPAALPSAVENIRQVVADGTPVFGICLGHQLLGL
                     AVGARTFKLKFGHRGTNHPVQQVETGRVEITSQNHGFAVDPATLPADVVVTHVNLYDG
                     TLEGFRHRTKPAFGVQYHPEASPGPHDADYLFGLFTEAMERR"
     gene            complement(3331..4122)
                     /locus_tag="PLT35_07910"
     CDS             complement(3331..4122)
                     /locus_tag="PLT35_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG29190.1"
                     /translation="MLGYWLGGRPSSSPSPRPAASAAVASARTGAGRVETPAARALAS
                     PEARVPEPEAATPADVNHGLSGGGRRDGAAAAPPASAPAASSASPPGSAPSAGTGTIV
                     VVASGRPANVYVDGRREGLTPRNLARVPFGPHSVRVTRPGYEAQEKRVTLSREEPAVR
                     LTFDLRPLAAAPARQPGEPERAETTPAASGRQTLVVETQPAGARVRVGGRDYGVTPVT
                     ITPIRTGLHKIELRLGGYRPWAETITVKAGQVRRVSVPLERTNSR"
BASE COUNT          700 a         1788 c         1685 g          660 t
ORIGIN      
        1 ccccagggtc gtagacgatg gccatcgccc ggccgccaag cacgtacgag ggcctgacca
       61 cgacggggaa gccgatgccg acggccacgt cccgcgcttc ggctcgcgac gatgccgtgc
      121 cgctcggcgg ctgcgggatg tcgagatccc ggaggagcgc ggcgaaccgc ttgcggtcct
      181 ccgcgaggtc gatcgagtcc ggcgaggtgc cgagcacggg caccccggcg cgatgcagcg
      241 ccgtggagag cttcagcggc gtctggcccc cgaactgcac gaggcaggcg acgctcccgc
      301 cagcttcccg ctccttgtcg atgaccgcca gcacgtcctc gagggtcagc ggctcgaagt
      361 agagccgatc ggcggtgtcg tagtcggtgg acaccgtctc ggggttgcag ttcaccatga
      421 cggtctcgaa gccctcgtcc cgcaggccga agaccgcgtg gcagcagcag tagtcgaact
      481 cgatgccctg gccgatccgg ttcggcccgc tgccgaggat gacggccttg cggtgcggcg
      541 tggggtcggc ctcgcactcc tgctcgtacg agccgtacag gtagggcgtc agcgactcga
      601 actcggcggc gcaggtgtcg atgcgcttgt aggacgcccg gagccccgcc gcagcccgcc
      661 gctcgcgcac ggcgcgctcg ctcgcgccgg tggcggccgc gatcgtcgcg tcgccgaacc
      721 cggcccgctt gagcgcccgc aaatccgcgc cgcccaggtc ctgcagcgtc ttgccgcgaa
      781 gcgcgcggcg ccgcgcagcc aggtccgcga actgcgcgag gaaccagggg tcgatccgcg
      841 tcagctcgtg cagcgccgtc gcgctccagc cgcggtcgag cgcctcgaac accgcccaca
      901 accgcccgtc ggtcggcgtc gccagccgct gccgcaggcc gtcgtcgtcc tcgccgcgca
      961 gccactcgtg ctcgaagagc aggctctccc gccccatctc gagcgaccgg acccccttca
     1021 tgaaggcgtc ctggaacgtc cgcccgatgg ccatgacctc cccgaccgac ttcatctgcg
     1081 tcgtcagggt gcgctcggcc tcggggaact tctcgaagtt ccaccgcggg atcttgacga
     1141 cgacgtagtc gattgtcggc tcgaaggacg cgggcgtcag gcgggtgatg tcgttcggga
     1201 tctcatcgag gtggtacccc agggccagct tcgccgcgat cttggcgatg ggaaacccgg
     1261 tcgccttcga cgcgagcgcg gacgagcgcg acacgcgcgg gttcatctcg atcacgacca
     1321 tgcgcccgtc gcgcggatcg agcgcgaact ggatgttcga tccgccggtc tcgacgccga
     1381 cgcgccggat gatccggcgc gccgcgtccc gcatcgcctg gtactctttg tcggtcagcg
     1441 tgagcgcggg cgccacggtg acgctgtcgc cggtgtgcac ccccatcggg tccaggttct
     1501 cgatcgagca gataacgacg aagttgtcgc cggcgtcgcg catcacctcc agctcgtatt
     1561 ccttccagcc gatgagcgac tcctcgatca ggacctcgtg caccgggctc atggcgaggc
     1621 cgcggcggac gatctcggtg aactcctcga tgttgtacgc gatcccgctc ccgacgccgc
     1681 cgagggtgaa cgacggccgg atgatcgacg ggaagccggt cgtgcggacg agcgacaggg
     1741 cctcgtccag cgaccgggcg tagccgctct cgggcacgcc cacgccgatc tcgcgcatgg
     1801 cgtcgcggaa cttgaggcgg tcctccgcga cgtcgatcgc ggccaccgac gctccgacga
     1861 gctccacgcc gtagcgcgcg agcgagccgg acttcgccag ggccatcgcc aggttgagcg
     1921 ccgtctgccc gcccaccgtg ggcagcagcg cgtcgggccg ctcgcgcgcg atcaccgcct
     1981 cgacgaaggc cggcgtcagg ggctcgacgt acgtccggtc cgccagctcc gggtcggtca
     2041 taatcgtcgc agggttgctg ttgaccagca ccacctcgac gccttccgcg cgcagcgcct
     2101 tgcacgcctg ggagccggaa tagtcgaact cgcacgcctg cccgatcacg atcgggccgg
     2161 cgccgagcac cagcacgcgc ttcacgtccg gccggcgcgg catctaccgg cgctccatgg
     2221 cctcggtgaa caggccgaac agatagtccg cgtcgtgggg gccgggcgac gcttccgggt
     2281 ggtactggac gccgaaggcg ggcttcgtgc gatgacggaa gccctccagc gtgccgtcgt
     2341 agaggttgac gtgcgtcacg acgacgtccg ccggcagcgt ggccgggtcc accgcaaagc
     2401 cgtggttctg cgacgtaatc tcgacgcgcc cggtctcgac ctgctgcacc gggtggttcg
     2461 tgccgcggtg cccgaacttc agcttgaagg tcctcgcacc gaccgccagg cccagcaact
     2521 ggtgccccag gcagatgccg aagacgggcg tgccgtccgc cacgacctgg cggatgttct
     2581 ccaccgccga cggcagcgcg gcgggatcgc cgggcccgtt gctcaggaag acacccgacg
     2641 gacgctcgga cagcaggacg gcggcgggcg tcgacgccgg atagactcgg atgtcgcacc
     2701 cgtacgacgc gagccgcctc aggatgttcc acttaatccc gaagtcccag gccgccacgc
     2761 gataccgtcg ggccggcgcc cggagcggct cgcgtccgta cccgccggtc cccgcggccg
     2821 ccccctcgac tggccagtcg aacggggcgg cgcacgtcac gccggacacc aggtcggagc
     2881 cggccatgag cggcacgcgt cgcgcctgtt cgacgagatc cgcgggtctg gcgtcaccgg
     2941 tcccaatgac gccgcgcatg accccagccg agcgcagccg gcgggtcagc gcgcgggtgt
     3001 cgatgtcgga gatggcgacc gtgcccgccg agacgaggta gtcccgcagc gtgcccgcag
     3061 cccgccagtt gctcgcgacg agcgactcgc ggcgcatgac gaacccggcg acctgcggag
     3121 cgcgcgactc ggcgtcttcc ggcgtgacgc cgtagttgcc gatttccggg caggtcatgg
     3181 tgacgatctg cccggcgtag gacggatcgg tcaggatctc ctgatagccc gtcaggccgg
     3241 tgttgaacac gacctcgccg accgcggtgc cttccgcccc ggccgccgtg ccctcgaacc
     3301 aggtgccatc ttcgagcgca agaacggccc tcaccgcgaa ttcgtcctct cgagaggcac
     3361 cgacacgcgg cggacctgcc cggccttgac cgtgatcgtc tccgcccagg gacggtagcc
     3421 gccgagcctg agttcgattt tgtggaggcc ggtccggatc ggcgtgatgg tgacaggcgt
     3481 caccccgtag tcgcgcccgc cgacgcggac ccgcgcccca gccggctgtg tctcgacgac
     3541 gagcgtctgc cggccggaag ctgcaggcgt cgtctcggcg cgctccggct cccccggctg
     3601 ccgcgccgga gccgccgcga gcggccgcag gtcgaatgtc agccgcaccg caggctcctc
     3661 gcggctcaac gtcacgcgct tctcctgggc ctcgtacccg gggcgcgtca cccgcacgct
     3721 gtgcggcccg aacggcacgc gagcgaggtt gcgcggcgtg aggccttcgc gccgcccatc
     3781 gacgtacacg ttcgcgggcc gacctgaggc gacgacgaca atcgtgcccg tgccggcaga
     3841 cggcgcggag cccggcggcg atgccgagct ggcggccggc gcggaagccg gaggggcagc
     3901 tgccgctccg tcccgcctgc cgccgcccga cagcccgtga ttgacgtcgg cgggcgtggc
     3961 ggcctctggc tctggaacgc gagcctcggg cgaagccagt gcgcgagcag ccggcgtttc
     4021 gacacgaccc gcgccagtcc gggccgaggc gacggcagcg ctggccgccg gccgcggact
     4081 gggcgaagag gacggacgcc cgccaagcca ataccccagc atcaggcccg ctgcgagtcc
     4141 cgccgcgagc ccagcggcga gacccgcggc gaacggcagc ctgcgccgct cgcgactcgc
     4201 tccagaccac tccatctcga agggcggccg ctccgccggc cggagcggtt cactcggcgc
     4261 gtcgaaaacg ctcgacggag ccgtcatggc tcgagccggt tcaccgtcgc cggtgccggc
     4321 cggatccgga atccggatcg gagccctgcg ccgcaggctc gcgggccggg actcgaccgg
     4381 ttctccggcc ggctgcgcgc cgtcgagaac ggcagccatc cccaacgccg ctggctcggg
     4441 tggtgggagc gcgagatcca gaccagccag atcgacggaa aacatgctcg ccggcgtctc
     4501 gctctcaaag ctgttcagct cggcggcgtc cagggaaaaa ccctcggctg agaacccgag
     4561 ctgctccgcc agatcaccgg atggcgggct cgaaacggcg tctgcctggg cctcggcgga
     4621 ttgaggcggc gccaaagccg acgcctcgcc gggccccggt gtcaggccga tcatcgcctc
     4681 gggcaccgag cccgccggct ccatcgcctc aatggccgcg gcagaagccg cctcggcgag
     4741 caggacgggc gcgccaagcc cgggagccgc cgccatgtcg ccctgagaag acggcgcgcc
     4801 gccggccggg gtatcgccag gctccggcga cgc
//
LOCUS       DAOSIF010000207         4829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15649_length_4829_cov_3.445748, whole genome shotgun sequence.
ACCESSION   DAOSIF010000207 DAOSIF010000000
VERSION     DAOSIF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15649_length_4829_cov_3.445748"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..372)
                     /locus_tag="PLT35_07915"
     CDS             complement(7..372)
                     /locus_tag="PLT35_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29191.1"
                     /translation="MSTLDGNNLFGSGPHEIHPESWQRAVMRRAFAALDGELAMDLGL
                     RSRRIVQTGRLQAAGSDVLNLLLAQIEWLLDGQLHTLVDSYGRVYDRVLVEEFAPASA
                     IRHGRGFWCDYRIVYRQLP"
     gene            complement(369..776)
                     /locus_tag="PLT35_07920"
     CDS             complement(369..776)
                     /locus_tag="PLT35_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29192.1"
                     /translation="MNRYTRIQSVTFDGVDLPLPLQVRVSRRCEPQAAAGDGDAFVTS
                     VQCSRPVLAAEVRLRGTAAAEALSPGQIGTLTFVVGPAAAGQAPRRVTLAGAVLTGAD
                     VAYEQAGFAEATLRFVAEAADGLTDPHAAEDAQ"
     gene            complement(773..1201)
                     /locus_tag="PLT35_07925"
     CDS             complement(773..1201)
                     /locus_tag="PLT35_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29193.1"
                     /translation="MPSPVADILNDIVETLRGTGRFALVTLGECAAATATPRACVTLA
                     GLDELRPDDAPGARWFRLRARVTVHARAHEPPGGTIRAAELCDAASALLADPFRAGRC
                     QHLPTGAATEIGFAEPAALRRPEGEMCLSVRCHFQTQDQP"
     gene            complement(1203..1856)
                     /locus_tag="PLT35_07930"
     CDS             complement(1203..1856)
                     /locus_tag="PLT35_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29194.1"
                     /translation="MSEFCQDRDLLGIEPGLFLGGGFAAQQLVGGSDGQLSGTTFTSP
                     GSDFSAAGVTAGMVLCVYDASPDEGVPLEILAVPSATTLTVSVLRAEADGPAVPPASA
                     EGLSFRVRTFRPQIRNASAALAEKLRQLAETAGVQSADFADSSQLCLTAAYLTLAGVF
                     VAQAQNAAAHDAHWIKAEHYRRESVRMQLQLRLAADEDGDGFAERTRTLGHVTLRRS"
     gene            complement(1989..3149)
                     /locus_tag="PLT35_07935"
     CDS             complement(1989..3149)
                     /locus_tag="PLT35_07935"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HOG29195.1"
                     /translation="MAFTGKATYSAGATLPELAEDVSDIVSIVSPYETPLLDHLGDAP
                     RAAGSTYHEWLEDELLPNTDAVNDDTWTDPAADTTFGVAHAARFRVGDQLRPHGSGEL
                     MLVTDVDTGAGTLTVTRGYGGSTPEDLSDAQVLHILGNAALEGDDAPAARFTTRVRKG
                     NWTQIFTSAVRVSGSDLAVRKVAVSDELDYQKQERLRELLRDLENTVVNGIAPVSNPQ
                     GGESVRRTMRGILPSLTTNVFSPGADGFPADTDLTEAQLNLALRSIWEQASSRIDTLV
                     VNGKQKRRINSFLTSGRAYGPADTSYRDLVSVYESDFGVCRVILCRAVPADTVLLLDS
                     GRIDVLPLAGRSFHYKPLASAGDYEAGEVIGEYTLELRNENAHGLITGLSIS"
     gene            complement(3179..3757)
                     /locus_tag="PLT35_07940"
     CDS             complement(3179..3757)
                     /locus_tag="PLT35_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29196.1"
                     /translation="MTEQQDIPVEAEPRDDSPAESPAPGRLVPVAESIRYRRRAQQAE
                     ARIHDLEQQFREVQAQLEDQRNQLALAEAQRDEARQQILFAENRIAAERALVESGVTD
                     LEAASILLSKRVDLNEPLGADALGRHVEQLLLDKPYLRAAAGGLPPPTAAPKPRQAPA
                     ADLADAARRAAHSGNRRDVADYLRLRRQLATH"
     gene            complement(3790..>4829)
                     /locus_tag="PLT35_07945"
     CDS             complement(3790..>4829)
                     /locus_tag="PLT35_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOG29197.1"
                     /translation="RRQGDASASDGRQDDGTDPAPARSNTSLARAIRFARTLRLETIE
                     AGRVLPILDENDYRRVRYWVQRFEKHPPRLASQDRPWWRLGRGPAAPSPETVEVVEIL
                     GPTWWQRYENGRLAVEGPNALGRLPVVHIPNLAVPGAYEGLSDVEPLIPLQDELNTRL
                     SDRAHRVTYQSFRMYLGKGIDDFLERPVGPGQMWATTNIDASIQEFGADSGSPSEDAH
                     IDQVRQAMDKVSGVTPLAAGLIRGNVGNLTSAAALKVVLAGLLARTARKRLTYGEGLQ
                     RIVEMSLAVLDRFGALRTAPADRRVEIHWPSPLPSDESEQLRNAQIKAQLGVPAERIL
                     AELGYGPENNE"
BASE COUNT          621 a         1586 c         1863 g          759 t
ORIGIN      
        1 cgtcgctcat ggcaattgtc tgtagacgat gcggtaatcg caccagaagc cccggccgtg
       61 ccggatggcc gaggccgggg cgaattcctc aacgagcacg cgatcgtaga cgcgcccgta
      121 ggaatcgacg agcgtgtgca gttggccgtc gaggagccac tcgatctgcg ccagcagcag
      181 gttgagcacg tcgcttccgg ccgcctggag ccgcccggtc tggacgatgc gtcgcgagcg
      241 caggcccagg tccatcgcca gctcgccatc gagagcggcg aaggcgcggc gcatcacggc
      301 acgctgccag gactcggggt ggatctcgtg cggtccgctg ccgaagaggt tgtttccgtc
      361 gagcgtgctc attgggcgtc ctccgcggcg tgggggtcgg tcaggccgtc ggcggcctcg
      421 gcgacaaacc gcagcgtcgc ctcggcgaag cccgcctgct cgtaggcgac atccgcgccc
      481 gtcaggaccg cgccggcgag ggtgacgcgg cgcggcgcct ggcccgcggc ggcagggccg
      541 acgacgaacg tcagcgtccc gatctgcccc ggcgacagcg cctccgccgc ggccgtgccg
      601 cgcaggcgca cctccgccgc caggaccggc cgggagcact gaacgctcgt gacgaaggcg
      661 tcgccgtcgc cggctgccgc ctgcggctcg caccggcgcg acacgcgcac ctggagcggc
      721 agggggaggt ccacgccgtc gaacgtcacg gactggatgc gcgtgtagcg gttcatggct
      781 ggtcctgcgt ctggaagtgg cagcggacgg aaaggcacat ttccccctcg ggtcgtcgca
      841 gcgctgcggg ttcggcgaag ccgatttcgg tggcggcgcc ggtaggcagg tgttggcagc
      901 gcccggcgcg gaaggggtcg gccagcaggg cgctcgccgc gtcgcacagt tccgccgcgc
      961 ggatcgttcc gcccggcggc tcatgcgcgc gggcgtgcac cgtcacgcgg gcccgcaggc
     1021 ggaaccagcg ggcgccgggc gcgtcgtcgg ggcgaagttc atcgagcccg gcgagtgtca
     1081 cgcaggcacg cggcgttgcc gtcgccgcgg cgcactcgcc cagcgtcacc agcgcgaacc
     1141 gccccgtgcc ccggagcgtc tcgacgatgt cgttcaggat gtccgccacg ggtgagggca
     1201 tctcacgacc tcctcagcgt cacgtgcccg agggttcgcg tccgctcggc gaagccgtcg
     1261 ccgtcctcgt cggccgccag gcggagctgg agctgcatgc ggacggactc gcggcggtaa
     1321 tgctcggcct tgatccagtg ggcgtcgtgg gcggcggcgt tttgggcctg ggccacgaac
     1381 acgcccgcca gcgtgagata cgcagccgtc aggcaaagct gcgacgagtc ggcgaagtcg
     1441 gcggactgca cgccggcggt ctcggcgagc tggcggagct tttcggccag cgcggccgag
     1501 gcgttgcgaa tctgcgggcg gaacgtgcgg acgcggaacg acaggccctc ggcggacgcg
     1561 ggcggaacgg ccgggccgtc ggcctcggcg cggagcacgg agaccgtcag cgtcgtcgcg
     1621 gagggcacgg cgaggatttc cagcggcacg ccctcgtccg gcgaggcgtc gtagacgcac
     1681 aggaccatgc cggccgtcac gccggcggcg gaaaagtcgc ttccgggcga ggtgaaggtc
     1741 gtgccggaaa gctgtccgtc ggagccgccg acgagttgct gggcggcaaa gcccccgccg
     1801 agaaacagcc ctggctcgat gcccagcagg tccctgtcct ggcagaattc gctcatggtc
     1861 gtttcctcgt tggtggtcgg gtgcgaggcg cggggtttgt cccgcccagt ctgccggtcg
     1921 ccggtccgct gtcgcgggcg tggcgcgggc gggacaggcc cggcgcctcg cgccggggtt
     1981 cgatgccgct aggagatgct caggccggtg atgagcccgt gggcgttctc gttgcggagt
     2041 tccagcgtgt attcgccgat gacctcgccg gcctcgtagt cgccggcgct ggccagcggc
     2101 ttgtagtgga agctgcgccc ggccagcggc agcacgtcga tccgtccgga gtccagcagc
     2161 agcaccgtgt cggcgggcac ggcccggcag aggatgacgc ggcagacgcc gaagtcgctc
     2221 tcgtagacgc tcaccaggtc gcggtacgac gtgtcggccg gtccgtacgc ccgaccggag
     2281 gtgaggaacg agttgatccg ccgcttctgc tttccgttga cgacgagggt gtcgatgcgg
     2341 ctggacgcct gctcccagat gctccgcagt gcgaggttga gctgcgcctc ggtgaggtcg
     2401 gtgtcggcgg ggaatccgtc ggcgccgggc gaaaagacgt ttgtcgtcag gctgggcagg
     2461 attccgcgca tcgtccggcg gacggactcg ccgccctgcg ggttgctgac gggggcgatg
     2521 ccgttgacga cggtgttctc caggtcgcgg agcagttcgc ggaggcgctc ctgcttctgg
     2581 tagtccagtt cgtcgctgac ggcgaccttg cggacggcca ggtcggaccc gctgacgcgg
     2641 acggcggagg tgaagatctg cgtccagttt cccttgcgga cgcgggtggt gaagcgggcg
     2701 gccggggcgt cgtcgccctc gagggcggcg ttgcccagga tgtgcagcac ctgggcgtcg
     2761 gaaaggtctt cgggcgtgga gccgccgtag ccgcgagtga cggtgagggt gccggcgccg
     2821 gtgtcgacgt ccgtcacgag catcaactcg ccgctgccgt ggggacgcag ctggtcgccg
     2881 acgcggaatc gcgccgcgtg cgccacgccg aaggtcgtgt cggcggcggg gtcggtccac
     2941 gtgtcatcgt tgacggcgtc ggtgttgggc agcagctcgt cctcgagcca ctcgtggtag
     3001 gtgctgccgg cggcgcgggg cgcgtcgccc aggtggtcca gcaggggcgt ctcgtacggc
     3061 gagacgatgc tgacgatgtc cgacacgtcc tcggcgagtt ccggcagcgt cgcgccggcc
     3121 gagtaggttg ccttgcctgt gaatgccatg gatggtttct cctgtgtggg aaaaggggtc
     3181 agtgggttgc cagttgcctg cggagacgca ggtagtcggc gacgtcgcgc cggtttccgc
     3241 tgtgggcggc gcgtcgcgcg gcgtcggcca ggtcggccgc gggagcctgc cggggcttcg
     3301 gggcggccgt cggcggggga agcccgcccg ccgccgcgcg aaggtacggc ttgtcgagca
     3361 ggagttgctc gacgtgccgg ccgagggcgt cggccccgag cggctcgttg aggtccacgc
     3421 gcttggacag cagaatcgac gccgcctcaa gatcggtcac gccggactcg acgagggccc
     3481 tctcggccgc gatgcggttc tcggcgaaga gaatctgctg acgggcctcg tcgcgctggg
     3541 cctcggccag cgcgagctgg ttgcgctggt cctcgagctg cgcctggact tcgcggaact
     3601 gctgttccag atcgtgaatc cgggcctcgg cctgctgcgc ccgccggcga taccggatgg
     3661 actcggccac cggcacgagc cgtccggggg cggggctttc ggcggggctg tcgtcccgcg
     3721 gttcggcctc gaccgggata tcctgctgct ctgtcatggg ggttcctttc gcttggtttt
     3781 tgcgcggggt cattcgttgt tctcaggtcc gtagccgagc tcggcgagaa tccgttccgc
     3841 gggcacgccg agctgcgcct tgatctgggc gttgcggagc tgctccgact cgtcgctggg
     3901 cagcgggctg ggccagtgga tctcgacgcg gcggtcggct ggcgcggttc gcagcgcgcc
     3961 gaaccggtcg agcacggcca ggctcatttc cacgatccgc tggagcccct cgccgtaggt
     4021 caggcgcttg cgggcggtgc gggcgagcag gccggccagc accaccttca gcgccgcagc
     4081 gctggtgagg ttgcccacgt tcccgcggat cagccctgcc gccagcggcg tgacgccgga
     4141 gaccttgtcc atcgcctggc ggacctggtc gatgtgggcg tcctcgctgg gcgagccgct
     4201 gtcggcgccg aattcctgga tggaggcgtc gatgttcgtg gtggcccaca tctgccccgg
     4261 gccgaccggg cgctccagga agtcgtcgat gcccttgccc aggtacatgc ggaacgactg
     4321 gtacgtgacg cgatgcgccc ggtcgctcag gcgggtgttg agttcgtcct ggaggggaat
     4381 gagcggttcg acgtcgctga gcccctcgta cgcgccgggg acggcgaggt tgggaatgtg
     4441 cacgacgggc agccgcccga gcgcgttggg accctcgacc gccaggcggc cgttctcata
     4501 ccgttgccac cacgtcgggc cgagaatctc gacgacctcg acggtttcgg gggacggagc
     4561 ggccgggccg cggcccagac gccaccacgg gcgatcctgc gaggccaggc ggggcgggtg
     4621 cttctcgaac cgctgcaccc agtagcggac gcggcggtaa tcgttctcgt ccaggatggg
     4681 caggacccgc ccggcctcga tggtctcgag ccgaagcgtg cgggcgaacc ggatggcccg
     4741 tgcaagggag gtgttagacc gggccggcgc ggggtccgtg ccgtcatcct gtcggccgtc
     4801 ggaggccgag gcatcgccct gccggcgcg
//
LOCUS       DAOSIF010000208         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15702_length_4816_cov_3.463558, whole genome shotgun sequence.
ACCESSION   DAOSIF010000208 DAOSIF010000000
VERSION     DAOSIF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15702_length_4816_cov_3.463558"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLT35_07950"
     CDS             <1..410
                     /locus_tag="PLT35_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29198.1"
                     /translation="GHLVFTTVHANNVVDVLAQRLVRVICPHCKRPSPPGPTLLEESG
                     LEPASVEDWTFYEGSGCIECGGTGYKGRTAICELLDLSDSIRELILARKPTSEIKKAA
                     REEGMRFLRECAVERVMQGLTTLREINKVTFVE"
     gene            407..1357
                     /gene="pilM"
                     /locus_tag="PLT35_07955"
     CDS             407..1357
                     /gene="pilM"
                     /locus_tag="PLT35_07955"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOG29199.1"
                     /translation="MRLPAVLRSPPPRVALEIGPSRAAAVSVTGSAGRGVTVDAWAVE
                     PLPPGAVAPSLTAPNVADPAVLADAVGRLWQRLGLRPKRVALLVPDATAKVSIVRFKD
                     VPAKASDLDELVKFQVRKAVPFRLEDSQVSYAAGERSPDGQDFVVVQARREIVEEYER
                     ACLATGAAPGIVELASFGLAQCALVTSGGIAGDWLLIHAGSEDATIAIFRGRHAVFFR
                     NRSASGDGHLGELVHQTAMYYQDRLGGQGFARVFAGGVAAGDLSTPVWTAIQERLGAA
                     AEIVGAGGSLSVPARRGLAPGDLEAMAPALGVVASRWAEH"
     gene            1369..1962
                     /locus_tag="PLT35_07960"
     CDS             1369..1962
                     /locus_tag="PLT35_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29200.1"
                     /translation="MGRLNLSTRPFYNERAVNAALAAAAAGVLAVTALNLWGVYILSG
                     RRADLGARILRAETATRDLRAQAARIRAATSAREIESTVAAAQEANALIGRRVFSWTE
                     VLNQFETTLPPTVRISSIRPRVERDGTMTVGVTVLARSVEAVGTFIENLEKQGAFADV
                     LSREEFVNQAGLIQASLEGRYVPRTASAAAGEAEGAR"
     gene            1959..2555
                     /locus_tag="PLT35_07965"
     CDS             1959..2555
                     /locus_tag="PLT35_07965"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HOG29201.1"
                     /translation="MTGGLLRVLRDRRAAVLPLVVALVLNIAVFGLVVFPSFRVVAGA
                     EQRERAALDELADAEREHAAASRIRQDKERAERNLRTFYEEVLPADLAGARRATYVHL
                     AQLARDAGLRYQRRVEQPRPPATTGEDRSSTLARFDITMVLEGDYEGVRQFLREVEAS
                     DSFIVIDNVGLAEGSESSGDLVLTINMSTYYRAGSRER"
     gene            2545..3141
                     /locus_tag="PLT35_07970"
     CDS             2545..3141
                     /locus_tag="PLT35_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29202.1"
                     /translation="MNASRRQVALLAAILTLLAAVLYYQYTVGGASPEELAAAARRAP
                     AAAAAGPAPARPAAVPAGIPAVDIAALERPQPEPSDTGRDPFHFGAAPIARAAAGPAG
                     SAAEGPRGVAPAVTGPAEPLGPPPPPPITLKFIGTARQGAGRFYAVLRDDRGVYYGAD
                     GDVVEGRYKVISVSADFVVLSYVDGRGRVSLPLSGGQP"
     gene            3166..>4816
                     /locus_tag="PLT35_07975"
     CDS             3166..>4816
                     /locus_tag="PLT35_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOG29203.1"
                     /translation="MTVVGRRSGRWRGAAALVAAALAVSAVSCASSRVFQHGNQAAMR
                     GDWDLAVTFYERAVKGSPNRADYKMALDRARLSAAQHHFEQARGYEKQDQLDLALLEY
                     RRVVEFHPGSQEARAKIQSVEQAIRSRIEAARPKPAVELMRQKARQAMDEPALNPASR
                     EPIEIRMPNRQIQEVLDFLGTASGINIAYDKDFPKTTVVSVNVAGVTLEEALDQILTV
                     NQAYYKVINPRSIIIIADSQAKRTAYDEQAVQTFYLSSAEAADIEQLLTKVIAAQQAG
                     VRPTFAANKAANSITVRATVPMLKMIERVIAMNDKPRAEISIDVELLEVNRSNARKHG
                     LDLSSWTLGLAFSPESRPSEKSAAFNLNTVSTGVSTLDFYATVPSATVAFLESDSATK
                     VIAKPNLRGSEGEKLSLKLGEELPVPSTTFYSAYGGTGAVATNPMTSFTYKNVGVNLD
                     IEPRVTYDGEIIMKLTLEVSSQSSDRNVAGQNLPAFATRKVETVLRLRDGESNLLAGL
                     LREDERTSLRGFPGATRAPILKQLFSANDNQVAQTDIVMLLT"
BASE COUNT          753 a         1665 c         1738 g          660 t
ORIGIN      
        1 cgggccatct cgtcttcacc accgtgcacg ccaacaacgt cgtggacgtc ctcgcgcagc
       61 gcctggtgcg cgtgatctgc ccccactgca agcggccctc accgccgggg cccacgctgc
      121 tggaggagtc cggactcgaa ccggcgagcg tggaggactg gacgttctac gaagggagcg
      181 gctgcatcga gtgcggcggc accggctaca agggccgcac ggccatctgc gagctgctgg
      241 acctctcgga ctcgattcgc gagctgatcc tggccaggaa gccgacctcg gagatcaaga
      301 aggcggcccg ggaggagggc atgcggttcc tgcgggaatg cgcggtggaa cgcgtcatgc
      361 aagggctgac aacgttgcgc gaaatcaaca aggtgacgtt cgtcgaatga ggctgccagc
      421 cgtcctccgc tcgccccccc cccgcgtggc cctcgagatc gggccctcac gggcggccgc
      481 cgtctctgtc accgggagcg ccggccgggg cgtgaccgtg gacgcctggg ccgtcgagcc
      541 gctgccgcca ggcgcggtgg cgccctccct gacggcgccg aacgtggcgg acccggccgt
      601 cctcgccgac gccgtcggcc ggttgtggca gcgcctcggc ctgcggccga agcgcgtggc
      661 cctgctcgtg ccggacgcca cggcgaaggt gtccatcgtc cgcttcaagg acgtgccggc
      721 gaaagcctcg gacctcgacg aactcgtgaa gttccaggtc cgcaaggccg ttccgtttcg
      781 actcgaggac tcgcaggtgt cgtacgcggc aggggaacgg agcccggacg gccaggactt
      841 cgtggtcgtg caggcccgcc gcgagatcgt cgaggagtac gagcgcgcct gcctcgcgac
      901 cggcgcggcg cctggcatcg tggagctggc gagcttcggc ctggcccagt gcgccctcgt
      961 cacgagcgga gggatcgcgg gcgactggct cctgatccac gcgggcagcg aggacgccac
     1021 catcgcgatc ttccggggcc ggcacgcggt gttcttcagg aaccgaagcg catcgggcga
     1081 cggccacctc ggcgagctcg tgcaccagac agcgatgtac taccaggatc ggctgggcgg
     1141 gcaggggttc gcccgggtct tcgccggcgg cgtggccgca ggcgatctgt cgacgcccgt
     1201 ctggacggcg atccaggagc gcctgggcgc cgcggcggag atcgtgggcg ccgggggcag
     1261 cctgtccgtg cccgccaggc gcgggctggc cccaggggac ctcgaagcca tggcgccggc
     1321 cctcggggtc gtcgcatcgc ggtgggctga gcactaggcg gaagaacgat gggtcgcctg
     1381 aacctgtcaa cccggccttt ctacaacgag cgcgcggtca acgcggcgct ggcggccgcc
     1441 gccgccggcg tcctcgccgt aacggccctc aacctctggg gggtctacat cctgtccggg
     1501 cggcgcgccg accttggcgc ccgcatcctc cgggccgaga cggcgacgcg cgacctgcgc
     1561 gcgcaggccg cccgcatccg ggccgcgacg agcgcccgtg agatcgagag caccgtggcg
     1621 gccgcgcagg aagccaacgc gttgatcggc cggcgcgtct tctcgtggac ggaggtgctc
     1681 aaccagttcg agacgacgct gcctccgacg gtccgcatct cgtcgatccg gccccgagtc
     1741 gagcgcgacg gcaccatgac ggtgggggtg accgtgctcg ccaggagcgt cgaggcggtc
     1801 gggaccttca tcgagaacct cgagaagcag ggcgccttcg cggacgtcct ctccagggag
     1861 gagttcgtga accaggccgg cctcatccag gccagcctgg aaggccgcta cgtgccgcgg
     1921 accgccagcg ccgccgcagg agaagcggag ggagcgcgat gacggggggg ctcctgcgcg
     1981 tgctccgaga tcgccgggcg gccgtgctgc cgctcgtggt cgcgctcgtg ctgaacatcg
     2041 cggtgttcgg cctcgtcgtg tttccctcgt tccgggtcgt ggccggcgcg gagcagcgcg
     2101 agcgggcggc gctcgatgaa ctggccgacg ccgagcggga gcacgctgcc gcctcgcgga
     2161 tccggcagga caaggagcgc gcggagcgga acctgcggac gttctacgag gaggtgctgc
     2221 ccgccgatct cgccggcgcc cggcgagcca cctacgtgca cctggcgcag ctcgccaggg
     2281 acgcgggcct ccggtaccag cggcgggtcg agcagccgcg gccgcccgca acgaccgggg
     2341 aggaccgctc ctcgacgctc gcccgcttcg acatcacgat ggtgctggag ggggactacg
     2401 aaggcgtccg ccagttcctg cgcgaggtcg aagcgtcgga ttcgttcatc gtgatcgaca
     2461 acgtgggact cgcggagggc tcggaatcga gcggagatct cgtgctgacg atcaacatgt
     2521 ccacctacta ccgggcggga tcccgtgaac gctagccgcc gccaggtcgc gctgctcgcg
     2581 gcgatcctca cgctgctggc cgcggtgctg tactaccagt acacagtcgg cggggcatcg
     2641 ccggaggagc tcgcggcggc ggcgcgccgc gccccggcgg ccgcggcggc ggggccggcg
     2701 ccggccaggc ctgccgcggt tcctgcgggg atccccgcgg tggacatcgc ggcgctggag
     2761 cggccccagc ccgagccttc ggacacgggc cgcgacccgt tccacttcgg cgcggctccg
     2821 atcgcgcgcg ccgcagccgg cccggcaggc tcggccgccg aagggcctcg cggcgtggcg
     2881 cccgcggtga cggggcctgc cgagccccta ggcccgccgc cgccgccgcc aatcacgttg
     2941 aagttcattg gcacggccag gcagggcgcg ggccgcttct acgcggtctt gcgcgacgac
     3001 cgcggcgtct attacggcgc cgacggcgac gtggtcgagg gacgctacaa ggtgatcagc
     3061 gtctcggccg acttcgtggt actgtcgtac gtcgatggcc ggggacgcgt aagcctcccg
     3121 ctgtccgggg gacagcccta gaccgcgccc gggatggaga gtcgcatgac ggtggtcggc
     3181 aggcgaagcg gtcggtggcg cggcgcggcg gcgctcgtcg cggccgcctt ggccgtgtcc
     3241 gcggtgagct gcgcgtccag ccgggtcttc cagcacggaa accaggccgc catgcgcggg
     3301 gattgggacc ttgcggtcac cttctacgag cgggcggtga aaggcagccc gaaccgggcc
     3361 gactacaaga tggccctcga ccgggcgcgc ctctcggcgg cccagcacca tttcgagcag
     3421 gcccgcgggt acgagaagca ggatcagctc gacctggcgc tcctcgagta ccggcgcgtg
     3481 gtggagttcc atcccggaag ccaggaggcg cgggcgaaga tccagagcgt cgagcaggcg
     3541 atcaggagcc ggatcgaggc ggcccgcccg aagcccgccg tggaactgat gcggcagaag
     3601 gcccgccagg cgatggacga gcccgctctg aacccggcgt cgcgcgagcc catcgagatc
     3661 cgcatgccga accggcagat ccaggaggtg ctcgacttcc tcggcaccgc ctccggcatc
     3721 aacatcgcct acgacaagga cttcccgaag acaaccgtgg tgagcgtcaa cgtcgcgggg
     3781 gtgacgctgg aggaggcgct cgaccagatc ctgacggtca accaggcgta ctacaaggtc
     3841 atcaacccgc ggagcatcat catcatcgcg gacagccagg ccaagcggac ggcgtacgac
     3901 gagcaggccg tgcagacgtt ctacctgtcc agcgccgagg cggcggacat cgagcagttg
     3961 ctgaccaagg tcatcgcggc ccagcaggcg ggcgtgcgcc cgaccttcgc cgcgaacaaa
     4021 gcggccaatt ccatcaccgt tcgggcgacc gtgccgatgc tgaagatgat cgagcgcgtg
     4081 atcgccatga acgacaagcc ccgagccgag atcagcattg acgtggagct gctcgaggtc
     4141 aaccgatcca acgcgcgcaa gcacgggctg gacctgtcgt cctggacgct gggcctggcg
     4201 ttttcgcccg agtcgaggcc gtccgagaaa tcggccgcct tcaacctcaa caccgtgtcg
     4261 acgggggtga gcaccctcga tttctacgcc acggtgccct cggccacggt ggcgttcctg
     4321 gagtccgatt cggcgaccaa ggtcatcgcc aagccgaacc tgcggggatc ggagggtgag
     4381 aaactctcgc tgaagctggg cgaggagctg ccggttccct ccacgacctt ctacagcgcg
     4441 tacggcggca ccggggcggt ggcgacgaac ccgatgacgt cgttcaccta caagaacgtc
     4501 ggcgtgaacc tcgacatcga gccgcgggtg acctacgacg gcgagatcat catgaagctg
     4561 acgctcgagg tcagctccca gagcagcgac cgcaacgtgg cgggccagaa cctgcccgcc
     4621 ttcgccacgc gcaaggtgga gaccgtgctc cgcctgcgcg acggagagtc gaacctgctg
     4681 gcgggtctgc tccgagaaga cgaacgcacg tcgctccgag gcttcccggg cgcgacgcgg
     4741 gcgcccatcc tgaagcagct gttttcggcc aacgacaatc aggtggccca aaccgacatc
     4801 gtgatgctgc tgacgc
//
LOCUS       DAOSIF010000209         4801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15772_length_4801_cov_3.771386, whole genome shotgun sequence.
ACCESSION   DAOSIF010000209 DAOSIF010000000
VERSION     DAOSIF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15772_length_4801_cov_3.771386"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..873
                     /locus_tag="PLT35_07980"
     CDS             427..873
                     /locus_tag="PLT35_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29204.1"
                     /translation="MQKIAGPKIVLLVSGGTPRPDGSPGRWYAPIGDAFAAAEIFLYT
                     TYVEQPEFGQARYGTSPTGAQDRLVELEGLENATSAAGGLFVEAIGAWDQHFDRVLAE
                     LSGSYLLGVEVEPADRDGRPHNVTVRVRRDGTAVRARAKYVIQPRQ"
     gene            916..2535
                     /locus_tag="PLT35_07985"
     CDS             916..2535
                     /locus_tag="PLT35_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG29205.1"
                     /translation="MTAGPAAPPPAPAQPKPPSALVSLWRALTSWRTASVALLSFSSG
                     MPLGLVWYAIPDWMRSIGVDIRIVGLFTLAQAPWTLKVIWSPLMDRYVPPFWGRRRGW
                     MAITQVALTVIGLLFAGVGRHPDAIWIVGALALAVAFASASQDIAIDAYAVEVLRKDE
                     QGAAVGARVAFYRAALVVSGGAAITMAGRLGWPTVNAMLALLYLPMLFVTWRAPEPEE
                     AVVAPRSLRDAVWQPFLGFLSRHRALEILAFVLLYKLSDQLTQALTRPFLIDMGYNAD
                     HRGIALATVGMFATIIGAVVGGWITTLIGLGHSLWVFGFLQIFSNLGYYLVSLPGRPV
                     LPLMYGATSFELFTSGLGTGAFSVLLLRMTQKRFSATQYALFSSLFALPRLLAGPIAG
                     VSVDAMGWSWFFLGTLVLGIPGLVMLARFVPLGVREPVFTVEEVAPKPPLRPLQIALR
                     GAAGGVLIGLTGFVLDAALAAVKTMRESKGALGFDLGGAMRRLAHPATVSDWVEVGGL
                     VAFAIIGGLFVAALAAARRGAGREIALSADA"
     gene            2699..4600
                     /locus_tag="PLT35_07990"
     CDS             2699..4600
                     /locus_tag="PLT35_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29206.1"
                     /translation="MLSTRCPRGRLLALAAALLMAGPMTGLAQAPNPPAAPPQAPAAQ
                     AAAPAQAPQTAAQPQQPAPAAQPAPAAPAAQAQPQEKPADEAASKLDPDEKAFRDASA
                     MTDADKQIEALRKVIADFPQSTFVASAENLIVTALTRKATTDVKALQEEAKKYSAGGA
                     TPNEQARRLAQLASGLSGAGVLLDEAEEYAKKALELLGDQDAWVAAEKKAAADAAERA
                     AKKDPAAKPRPEVSEADYVSRFVTQRQGGLVTLAQIYEKRGKAADAEKTYRDAFALQP
                     QTGGAAALKLAEFAKAAGHPFEQLEYLTVATLAGRVTAASRGELEALYEATRGGSSAD
                     LDRMLDERYAQSVTKVHAPAYVRPKGRTGRMVLAELFTGAGCPPCVGADLAFDGALEG
                     YSPRDFALLVYHLHIPRPDPMTNPSTEARKGFYDVPGTPTFFVDGGDRHVGGGNAAGA
                     QKLFDETIRPAVEKRLDARPGAVIRLEAALKGDTVSAQAKVSKTGKPGRKLRVQIALV
                     EEHVRYTGENGVRFHSMVVRSLASAEKDTLGFAAASGKGTTLRHTFDLGAVEAGAKAH
                     LDAMEGGSSKRFGTFQFVERKSALDRSRLRVVVFVQDEETKEVLQAAVQPIRAARAAA
                     GAGSAGHRP"
     gene            4597..>4801
                     /locus_tag="PLT35_07995"
     CDS             4597..>4801
                     /locus_tag="PLT35_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29207.1"
                     /translation="MTPGRAARIACGLAAAVVVASAAAIWRAQPARQGSGDGRAGAGG
                     PRGAAETARHAGGGADASAAGATY"
BASE COUNT          632 a         1713 c         1784 g          672 t
ORIGIN      
        1 gcgcgcggca tcttccgcag catcggtcgg cgcctcgtgc gctcgccgct ccagttcgtg
       61 cgctggccga gaggcagcgc gtagcccgcc ggccgatgcg gacggcttca gagcggtgcg
      121 gagcggacat gcgcccgcgc gcccaggcgg acaacgcctt cgcgcgtgca tgagatcggc
      181 gggtgaccgc gagcatcggg actcccaccc ccgttccccg gacttccgcc cagccgaagc
      241 ccccgagcgc cctcgtcggc ctctggcgcg tgctgacctc ctggcgcacc gcctcggtcc
      301 cttcaagagg cgcgccaggc aggcggttcc gctggtccgc gttcgcgggt gcggagactc
      361 tctgtgaagg aatcctgcct gcgcggggac cggtcgctta gtgccctccg ggatctcgga
      421 ctggccttgc agaagatcgc tggaccgaag atcgtcctgc tggtttccgg aggcaccccg
      481 aggccggatg gttcaccagg caggtggtac gcgcccatcg gcgacgcgtt cgctgcagcc
      541 gagatcttcc tgtacacgac gtacgtggaa cagcccgagt tcggtcaggc acgatacggc
      601 acctccccga ccggggcgca ggaccggctg gtggaactcg aagggctcga gaacgcgaca
      661 tcggcggctg gcggcctgtt cgtcgaggcc attggcgcgt gggaccagca cttcgaccgt
      721 gtcctcgccg agctttccgg gtcgtacctg ctgggagtcg aagtagagcc ggcggatcgc
      781 gacggccggc cccacaacgt gacggttcgc gtgcggcgcg acggtacggc cgtgcgtgcg
      841 cgggccaagt acgtcatcca gccgcgtcag tagatccccg cgggactccg cctccctcgc
      901 gtgtatgatc ggcgggtgac tgccggcccc gccgcgcccc ctcccgctcc cgcccagccg
      961 aagcccccga gcgctctcgt cagcctctgg cgcgcgctga cctcctggcg caccgcctcg
     1021 gtcgccctgc tgtccttctc ctcgggcatg ccgctgggcc tggtctggta cgccatcccc
     1081 gactggatgc gcagcatcgg cgtggacatc cgcatcgtcg gcttgttcac gctggcccag
     1141 gcgccgtgga cgctgaaggt gatctggtcg ccgctgatgg atcgctacgt gccgcccttc
     1201 tggggacggc ggcgcgggtg gatggccatc acgcaggtgg cgctcaccgt gatcggcctg
     1261 ctgttcgcgg gagtcgggcg ccacccggac gcgatctgga tcgtcggcgc gctggcgctg
     1321 gcggtggcct tcgcgtcggc gtcgcaggac atcgccatcg acgcgtacgc ggtcgaggtg
     1381 ctgcgcaagg acgagcaggg cgccgccgtc ggggcccgcg tcgcgttcta ccgcgcggcc
     1441 ctggtcgtct ccggcggcgc cgcgatcacg atggcagggc ggctcggatg gccgacggtc
     1501 aatgccatgc tggccctgct ctacctcccg atgctcttcg tcacgtggag ggcgccggag
     1561 ccggaagagg ccgtcgtcgc gccgaggagc ctgcgcgacg ccgtctggca gcccttcctg
     1621 ggcttcctgt cgcgccaccg cgccctcgag atcctcgcct tcgtcctgct ctacaagctc
     1681 tccgatcagc tcacgcaggc gctgacgcgg ccgttcctga tcgacatggg ctacaacgcc
     1741 gatcaccgcg gcatcgcgct ggccacggtc ggcatgttcg cgacgatcat cggggcggta
     1801 gtgggcggct ggatcacgac gctcatcggc ctcgggcact cgctgtgggt gttcggcttc
     1861 ctgcagatct tctcgaacct cggctactac ctcgtgtcgc tgcccggccg tcccgtgctg
     1921 ccgctcatgt acggcgccac gagcttcgag ctgttcacgt cgggccttgg gacgggcgcc
     1981 ttctcggtgc tcctcttgcg catgacgcag aagcgcttct cggccacgca gtacgcgctc
     2041 ttctcgagcc tgttcgcgct gccgcgcctg ctggccgggc ccatcgcggg cgtgtcggtg
     2101 gacgcgatgg gctggtcgtg gttcttcctc ggcacgctcg tcctcggcat ccccggcctg
     2161 gtgatgctcg cgcgcttcgt cccgctgggc gtgcgcgagc cggtcttcac cgtcgaggag
     2221 gtcgccccga agcctccgct ccggccgctg cagatcgcgc tgcgcggcgc cgctggcggc
     2281 gtcctcatcg gcctgacggg gttcgtgctc gacgcggcgc tggcggcggt gaagacgatg
     2341 cgcgagagca agggcgcgtt gggcttcgac ctgggcggag cgatgcggcg cctggcgcac
     2401 cccgcgacgg tctcggactg ggtggaggtc ggcggcctcg tcgcgttcgc gatcatcggc
     2461 ggcctgttcg tcgcggccct cgcggcagcc aggcgcggcg ccggccgcga gatcgctttg
     2521 tcagccgacg cctgatcggg cggcctgcca ggcgcagcgt tccggcgccc gaagcgcggc
     2581 gcctggcgcc gggtccgggc ccgcggccgg ggcaggctcc tgcgatagac tggatcggac
     2641 tggatgacgc gcggctccgt catgccggcg gcgcgcgggc ttcccgaaac gaggtcacat
     2701 gctcagcacc agatgccccc gcgggcggct cctcgcgctc gccgccgccc tgctgatggc
     2761 gggaccgatg acgggactcg cgcaggcccc caatccgccc gccgccccgc cgcaggcccc
     2821 ggcagcccag gcggcggcac ccgcgcaggc gccgcagacg gcggcgcagc cgcagcagcc
     2881 cgcgcccgcc gcgcagcccg cacccgccgc gcccgcggcc caggcgcagc cgcaggagaa
     2941 gccggccgac gaggcggcgt cgaagctcga tccggacgag aaggccttcc gggacgcctc
     3001 ggcgatgacg gacgcggaca agcagatcga ggcgctgcgg aaggtgatcg cggacttccc
     3061 gcagagcacg ttcgtggcga gcgccgagaa cctcattgtg accgcgctga cgaggaaggc
     3121 tacgaccgac gtgaaggccc tccaggagga ggcgaagaag tactcggccg gcggcgcgac
     3181 gccgaacgag caggcgcggc ggctcgcgca gctcgccagc ggcctgtccg gcgcgggcgt
     3241 gctcctggac gaggcggagg agtacgcgaa gaaggcgctg gagctgctgg gcgaccagga
     3301 cgcgtgggtg gccgccgaga agaaggcggc ggcggacgcg gccgagcgcg cggcgaagaa
     3361 agacccggcc gcgaagcccc ggccggaggt gagcgaggcg gactacgtgt cgcgcttcgt
     3421 cacgcagcgc cagggagggc tcgtgacgct ggcacagatt tacgagaagc gagggaaggc
     3481 cgccgacgcc gagaaaacgt accgcgacgc gttcgcgctg cagccgcaga ccggcggcgc
     3541 ggcggcgctg aagctggccg agttcgccaa ggccgcgggc catccgttcg agcagctcga
     3601 gtacctcacc gtggcgacgc ttgcgggccg cgtcaccgcg gcgtcgaggg gcgaactcga
     3661 ggcgctctac gaggccacgc gcggcgggtc ctcggcggac ctcgaccgca tgctggacga
     3721 gcggtacgcg caatcggtga cgaaggtcca cgcgccggcc tacgtccggc cgaaagggcg
     3781 cacgggccgc atggttctgg cggagctctt caccggcgcg ggctgcccgc cctgcgtcgg
     3841 ggccgacctc gccttcgacg gcgcgctcga ggggtactcg ccgcgggact tcgcgctgct
     3901 cgtctatcac ctgcacatcc cgcgcccgga tcccatgacg aacccgtcca ccgaggcccg
     3961 caagggcttc tacgacgtgc ccggcacgcc gacgttcttc gtggatggcg gcgaccggca
     4021 cgtgggcggc ggcaacgcgg ccggcgccca gaagctgttc gacgagacga tccggcccgc
     4081 cgtcgagaag cggctcgacg ccaggcccgg cgccgtgatt cgactcgagg ccgcgctgaa
     4141 gggcgacacg gtgtccgcgc aggcgaaggt ctcgaagacc gggaagccgg gacggaagct
     4201 gagggtccag atcgcgctgg tggaggagca cgtgcgctac accggcgaga acggcgtccg
     4261 gttccactcg atggtcgtgc ggagcctcgc gtcggccgag aaggacaccc tcggcttcgc
     4321 cgcggcctcc gggaagggaa cgacgctgcg gcacacgttc gacctcggcg cggtcgaggc
     4381 cggggccaaa gcccacctcg acgccatgga aggcggcagc agcaagcgct tcggcacgtt
     4441 ccagttcgtg gagcggaaga gcgccctcga ccggagccgc ctgcgcgtgg tggtgttcgt
     4501 gcaagacgag gagacgaagg aagtgctgca ggcggccgtc cagccgatcc gcgcggcgcg
     4561 ggcggcggct ggcgccggat cggcgggcca tcggccgtga cgccgggccg cgcggcccgc
     4621 atcgcctgcg gcctcgccgc tgccgttgtg gtggcgtcgg ccgctgcgat ctggcgcgcg
     4681 cagccggcgc gtcagggcag cggggacggg cgcgccggcg caggcgggcc gcgcggggcg
     4741 gccgagacgg cgcgccacgc gggcggcggc gccgacgcgt cggcggcggg cgcgacctac
     4801 g
//
LOCUS       DAOSIF010000210         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15828_length_4785_cov_5.224524, whole genome shotgun sequence.
ACCESSION   DAOSIF010000210 DAOSIF010000000
VERSION     DAOSIF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15828_length_4785_cov_5.224524"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLT35_08000"
     CDS             complement(<1..262)
                     /locus_tag="PLT35_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(221..223),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOG29208.1"
                     /translation="MVKRLTEMVSCSGUASKLAAGELAQVLGHIPPQKDPRILVDFRT
                     ADDAGVYEWAGGPALVQTVDFFTPIVDDPYAYGQIAAANALSD"
     gene            complement(366..1271)
                     /locus_tag="PLT35_08005"
     CDS             complement(366..1271)
                     /locus_tag="PLT35_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOG29209.1"
                     /translation="MAEKRHALGRGLDALIGDALEGAGRPAGGPFEVEIDRLSPNRLQ
                     PRSRVEDARIDELARSIRANGVIQPILVRQAPNGYQIIAGERRWRAAQRAGLLRVPVT
                     VRDVPDDQDRSLLLLALIENLQRVDLNPMAEAAAYQRLVDEFRLTQDEIAAAVGKDRS
                     TVANMLRLLKLPRDVRDDVAAGRLTTGHARALVALEGEEAQRNLAREAIAHGWSVRET
                     ERFVTRALARSGAAAARKPLGARTLDADTKAAQDKLRLVIGGRVDILRRGRGGEIRIG
                     FKNEEELIRIFEILVRDNQGLGPRR"
     gene            complement(1277..2083)
                     /locus_tag="PLT35_08010"
     CDS             complement(1277..2083)
                     /locus_tag="PLT35_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOG29210.1"
                     /translation="MARILAVANQKGGVGKTTTAINLGASLALADQRVLLVDADPQCN
                     LSSGVGLKGRSAPAGDVYDALMCDDIPDPAVYILPTSVSGLSAIPSDRNLAGAEIELV
                     PMLARESRLRRLVSPLRDRFDYILIDCPPSLGLLTLNALVACDAVLIPLHCEYFALEG
                     LADLVGTLKRVRAALNPSLDIDGVLLTMYDERTNLGQQVSRDVRAFFKEKVFRTVIPR
                     NVRLGEAPSHGMPVILYDVKSRGAEAYLALAREVLARNGRAPATPPTPTK"
     gene            complement(2330..3022)
                     /gene="rsmG"
                     /locus_tag="PLT35_08015"
     CDS             complement(2330..3022)
                     /gene="rsmG"
                     /locus_tag="PLT35_08015"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOG29211.1"
                     /translation="MTPREFGDRLAKRARRAGVALEPSIVESLAAYYQLLEVWNKKIN
                     LTAFPLSEGQDEAFDRLLIEPLVAAKHLQSTHGHGRPHGAVLGARPRLLDIGSGGGSP
                     AIPMKLAIPSLVLTMVESKTRKSAFLREAVRQLGLADAAVETARAEDLLTRSDLHESQ
                     DLVTVRAVRVEQRLLVHLQAFLRLGGRVLLFRTVSGADTPPLVAPPFVYEATWTLVDS
                     LRSRLVVLRKML"
     gene            complement(3000..>4785)
                     /gene="mnmG"
                     /locus_tag="PLT35_08020"
     CDS             complement(3000..>4785)
                     /gene="mnmG"
                     /locus_tag="PLT35_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOG29212.1"
                     /translation="TPSIESVARMSCNPAIGGVAKGHLVREIDALGGLMGRAIDATGI
                     QFKLLNRGRGPAVWSPRAQADKRSYAEWMKRALSEHPRVEWVSGLGGGLGRARGRTGK
                     GSEFWVLTLIHGWDVSARAVVLTTGTFLNGRIHVGNESWAGGRFGEPASIELANSLAK
                     LGFRMGRLKTGTPPRLRRESIDFEAGVRSGAFLPDPGDPDPVPMSFLSGRIVCEQITC
                     WHLHTTGAVHDVVRRNIYRSPLYNGQITGTGPRYCPSLEDKVMRFPERERHRIVLEPE
                     GRNVEEIYLNGLSMSLPRDVQAELIHALPGLSGAEVLRPAYAVEYDFVQPTELTATLE
                     AKRLAGLYLAGQINGTSGYEEAAAQGLVAGANAALTVAGRPPFTLGRADAYIGVLIDD
                     LVTQGCVEPYRMFTSRAEYRLLLRIDNADLRLTPLGREVGLVDDVRWKRFQERRERLH
                     RNLAALRDSRVEAGGLRIPAIQRLRQPGVRLSDMLEGGDLRLDVDPASAHYDLSTAET
                     IVKYHGYLKRQQQEVEKARREEERAIPRDLPYERIPGLSREMVQRLGEIGPRTLGQAG
                     RIPGVTPAAVAVVGAYLERFSHDATGIR"
BASE COUNT          773 a         1606 c         1641 g          765 t
ORIGIN      
        1 cgtcgctcag cgcgttcgcc gccgcgatct ggccgtacgc gtacgggtcg tccacgatgg
       61 gcgtgaagaa gtccaccgtc tggacgagcg ccggcccgcc ggcccattcg tacacaccgg
      121 catcgtccgc ggtgcggaag tcgacgagga tccgcgggtc tttctgaggc gggatgtggc
      181 ccaggacctg ggcgagctcg ccagcggcga gtttgctcgc tcagcccgag caggaaacca
      241 tctcagtgag tcgcttcacc atgtcacttc tccagggact aggggctagg gactgggcgc
      301 taggggctag accgtcgcgc tgcctcgctg caacccacgc ggcgagagcc gcgtcagacg
      361 agcgcctagc gcctaggccc cagcccctgg ttgtccctga ccagtatctc gaaaatccgg
      421 atcagctctt cttcgttctt gaacccgatc cggatttccc ctcctctgcc ccggcggagg
      481 atgtccacgc ggcccccgat gacgaggcgg agcttgtcct gagcggcctt cgtgtccgcg
      541 tcgagcgtcc gcgcgccgag gggcttgcgg gcggccgccg cgccgctgcg ggcgagcgcg
      601 cgcgtgacga accgctcggt ctcgcggacg gaccagccat gcgcgatcgc ctcgcgcgcg
      661 aggttgcgct gcgcctcctc gccctcgagc gcgacgagcg cgcgcgcgtg gcccgtcgtc
      721 aggcgtcccg cggccacgtc gtcgcggacg tccctgggga gcttgaggag ccgcagcatg
      781 ttggctaccg tcgagcggtc cttgccgacc gcggcggcaa tctcgtcctg agtgagcctg
      841 aactcgtcga ccagccgctg gtaggccgcg gcctcggcca tcggattgag gtccacgcgc
      901 tggaggttct cgatgagggc gagcagcagg aggctccggt cctggtcgtc cgggacgtcg
      961 cggacggtga ccgggacccg gaggaggccg gcgcgctgcg cggcgcgcca ccgccgctcg
     1021 cccgcgatga tctggtagcc gttgggcgcc tggcgcacga ggatgggctg gatgacgccg
     1081 ttggcgcgga tcgagcgcgc gagctcgtcg atgcgcgcgt cctcgacgcg ggaccgcggc
     1141 tgaaggcggt tcggggagag ccgatcgatc tcgacttcga acgggccgcc ggccggccgg
     1201 ccggcgccct cgagggcgtc gccgatgagg gcgtccagcc ccctgcccag tgcgtgtcgt
     1261 ttctcagcca tggtggtcac ttcgtcggcg tcggcggagt tgccggagcg cggccgttgc
     1321 gcgcgaggac ctcgcgcgcc agcgcgaggt acgcctcggc gccgcgggat ttcacgtcgt
     1381 agaggatgac cggcatgccg tggctcggcg cttcgcccag gcgcacgttg cgcgggatga
     1441 cggtgcggaa gaccttctct ttgaagaacg cgcggacgtc gcgcgacacc tgctggccca
     1501 ggttcgtccg ctcgtcgtac atcgtcagca gcacgccgtc gatgtcgagc gacggattga
     1561 gcgcggcgcg gacgcgcttg agggtgccga ccaggtcggc gaggccttcg agcgcgaagt
     1621 actcgcagtg cagcgggatg agcaccgcat cgcaggcgac gagggcgttg agggtgagca
     1681 ggccgagcga cggcgggcag tcgatgagga tgtagtcgaa gcggtcgcgg aggggcgaga
     1741 cgaggcggcg gagccgggac tcgcgcgcga gcatgggaac cagctcgatt tccgcgccgg
     1801 cgaggttgcg gtcggaagga atggcggaca ggccggagac ggaggtcggc aagatgtaga
     1861 cggccgggtc cgggatgtcg tcgcacatga gggcgtcgta gacgtcgccg gcgggagcgc
     1921 tgcggccttt gaggccgacg ccgctcgaga ggttgcactg cgggtcggcg tcgacgagca
     1981 ggacgcgctg gtcggcgagg gcgagcgagg cgccgaggtt gatggcggtg gtggtcttgc
     2041 cgacgccgcc tttctggttg gcgacggcga ggattctggc catggacagg gcccgggtcg
     2101 agaagtctca cgcgcgggcg cgggcccgcg ggcgaggcga ggtgacggtg cgagcgagcg
     2161 ggctcgcatg gctgggcggg ctggagggtt tgttttgggt gttcaacaga ataccagcct
     2221 gccttgtgtc gtacttgttg agaataacat ggatagcgac gagagatacg agaaatcccc
     2281 cgtctgttcc acgtgaaaca cccggccgga cacctggtct cgcatgcggt cagagcatct
     2341 tccggagcac gacgaggcgg ctgcggaggc tgtccacgag cgtccacgtg gcctcgtaga
     2401 cgaagggcgg cgcgacaagc ggcggcgtgt ccgcaccaga cacggttcgg aacaagagga
     2461 cacggccgcc gagcctgaga aacgcctgca ggtgcaccag caggcgctgc tccacccgga
     2521 cagcccggac ggtgacgaga tcctgcgact cgtgcaggtc ggaacgagta agcagatcct
     2581 cggccctcgc ggtctcaacg gcggcgtcgg caaggcccag ttgccggacg gcttccctga
     2641 gaaacgccga cttcctcgtc ttcgactcga ccatggtcag gacgagtgat ggaatcgcca
     2701 gcttcatggg aatggctggc gagcctcctc cagaaccaat gtccagcagg cgcggacggg
     2761 cccccaggac ggcaccgtgt ggccggccat gcccgtgggt gctctgcaga tgcttcgccg
     2821 cgacgagagg ctcgatcaga agccggtcga aagcctcgtc ctggccctcg ctgagcggga
     2881 acgccgtgag attgatcttc ttgttccaga cttcgagaag ctgatagtaa gccgccagcg
     2941 attcaacgat cgacggctcc agcgcgacgc ccgccctccg tgcccgcttc gccaggcgat
     3001 caccgaattc ccgtggcgtc atggctgaat cgctccagat acgcgccaac aacggcaacg
     3061 gcggccggcg tcaccccggg gattcggcca gcctggccga gcgtccgggg cccgatttca
     3121 cccagccgct ggaccatctc gcgggacaac ccggggattc tctcatacgg caagtcccgg
     3181 gggatagcgc gctcttcttc tcttcgcgcc ttttcgacct cctgctgctg gcgcttcaag
     3241 tagccgtggt acttcacgat cgtctccgct gttgacagat cgtagtgcgc agaagccgga
     3301 tccacgtcca gccgcagatc gccgccttcg agcatgtcag acagtcgaac ccccggctgc
     3361 cgcagacgct ggatcgccgg aatcctcagg ccgccggctt caacccgcga gtcccgcaag
     3421 gccgccaggt tccggtgcag cctctctcgg cgttcctgga agcgtttcca gcggacatca
     3481 tccacaagcc cgacttccct tccgagcggg gtcaggcgga ggtccgcgtt gtcaatccgc
     3541 aggagcaggc ggtattcggc tcgcgaggtg aacatccggt acggctcgac gcacccctgc
     3601 gtgacgaggt cgtcgatgag cacgccgata taggcgtcgg cgcgacccag ggtgaagggg
     3661 ggccgccccg caaccgtcag cgcggcattc gcaccggcaa ccaggccctg agcggccgct
     3721 tcctcgtacc cggacgttcc gttgatctgg cccgccagat acagccccgc cagtcgcttc
     3781 gcctcgagcg tcgccgtcag ctccgtaggc tgcacgaaat cgtactcgac ggcataggcg
     3841 ggccgcagca cctcggctcc cgagaggccg ggcagcgcgt ggatcagctc cgcctgcaca
     3901 tcgcggggca ggctcatcga cagcccgttc aggtagatct cttccacgtt ccgaccttca
     3961 ggctcgagga caatgcgatg acgctcgcgt tctggaaaac gcatcacctt gtcctcgagc
     4021 gacgggcagt accgcggacc cgtgcccgtg atttgcccgt tgtacagcgg cgatcgatag
     4081 atgttcctcc gtaccacgtc gtggactgcg ccggtcgtat gaagatgcca gcaggtgatc
     4141 tgctcgcaga cgatccggcc gctgagaaaa gacatcggca ccgggtcagg gtcgccggga
     4201 tcgggcagga acgcgccgct tcgcacgccg gcctcgaaat cgatgctctc tcggcgcaag
     4261 cggggcgggg tgccggtctt cagccgaccc atgcgaaaac ccaacttagc caacgagttg
     4321 gcgagctcaa tcgacgccgg ctccccgaat ctgccgccag cccacgactc gttgccgacg
     4381 tgaatccgcc cgttcaagaa cgtacccgtc gtcagaacca ctgctctcgc cgaaacatcc
     4441 catccatgaa ttagggttaa cacccaaaat tctgagccct tgcccgtcct tccacgcgcc
     4501 cgacccaggc cgccgcccag cccgctcacc cactccaccc gcggatgctc gctcaacgcc
     4561 cgcttcatcc attcggcgta cgaacgcttg tccgcctgcg cccgcggaga ccagacggcc
     4621 ggcccccggc cgcggttgag cagcttgaac tggatgcccg tcgcgtcaat cgcgcggccc
     4681 atcagccccc cgagcgcgtc gatctcccgg acgagatgcc ccttcgcgac gccgccaatg
     4741 gcaggattgc aggacatccg cgcgaccgat tcgatcgagg gcgtg
//
LOCUS       DAOSIF010000211         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15831_length_4785_cov_3.284567, whole genome shotgun sequence.
ACCESSION   DAOSIF010000211 DAOSIF010000000
VERSION     DAOSIF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15831_length_4785_cov_3.284567"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLT35_08025"
     CDS             <1..239
                     /locus_tag="PLT35_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29213.1"
                     /translation="ADASVPVAAQGRRSRGVAVGEGAWLGAGAKILDGVRIGSHAIIG
                     AGAIVSVDVPEYAVAVGRTARIAGSRREDAGPAE"
     gene            241..1440
                     /locus_tag="PLT35_08030"
     CDS             241..1440
                     /locus_tag="PLT35_08030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG29214.1"
                     /translation="MSARKLNILHVCDHLGWEGSRMHGVKRLFSWMMPRFDPDRYAVS
                     LVSLRRKDLSEETLESLGVDISYLHRSKFDPATLTALLKVIDRKAIDVLHLHGYGATT
                     FGRAAAALRRLPVVLHEHANLTDTPWFQKVADRALAPFTDIAIAVSRSTAEFLVRARL
                     VPERKVKVVYLGAPFDEFSRARTALEIAAAREALGIKDGDFAVGTVTRLHDSKGNQYL
                     VDAARLVLDARPQARFVLVGEGPLRPDLEAQAARLGLGDRFVFAGFARDVAAALSAFD
                     LSVFPSLWEGTPLTAFEALAAGKAIVATDADGLLDVLRDGRDARIVPKRDARALADRI
                     IELMDAPDERRRLGQHARTTGAEYDIAAFVRKMERLYALLHDVSRRTRRKGVLDEDLS
                     FLERSRA"
     gene            1437..3311
                     /locus_tag="PLT35_08035"
     CDS             1437..3311
                     /locus_tag="PLT35_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29215.1"
                     /translation="MTDRTAAARRPDARPGLLAAAAVVCVYGAVALSVDFPRAAFGIQ
                     SDEATYYMMGHSLVNDGDLAYRREDLARVWREFPSGPSGVFLKQGRDLDVSLSAAPPF
                     VRIDSRPDPDRARLFYGKSYIYPLAASPLVALFGTNGFLAFHALLLGLVTLAGYVFLN
                     ARSSPAVSLLLALGFLLASVVPAYFVWITPELFNFSLVFLGYFCWLYKEVAPAGTVSR
                     GARWLLTPSSDLAAAALIGVATFSKPSNLLLVLPLLIWLAARREWRRMAAAGLVFAAV
                     GAALLAGNVAVTGDWNFQGGDRRTFYGSFPLQERDLAWNDVGQGRATDRVLSEVIFDP
                     RVFWTVLGHNLGYVFAGRYSGLVPYFFPGVFALAAFLFARGRRAGWQWLVLGAAAAEI
                     LLLLVWVPYTYNGGGGSIGNRYFVNTYGTLLFLLPPIAGAGTALVPWLVGCLFSAQIT
                     FNPFFASFNPGDPAKHGPLRWLPVELTLVNDLPINTQANRVRIWFGEARRFQIYFLDD
                     NTYGREGLSFWVRGRARTEMLVKTVEPASALDLDLAAGDAATSVTVTRGRLSRRVDLA
                     PGASARVSLPLDEGFPYSGTRVWRVSIRSSGGFVPHFLTPGSEDKRFLGVRVTPELRP
                     "
     gene            3335..4375
                     /locus_tag="PLT35_08040"
     CDS             3335..4375
                     /locus_tag="PLT35_08040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG29216.1"
                     /translation="MKIGVIISSPPLAEGGHLVLARSLVEALRDAGHEAGLVVTPQNR
                     FGRLPSSYLAAWLTDVEVAYDGAPIHQVISLRFPAYAVRHPRHVVWLNHRMREYYDLW
                     ERFRATLSWRARLKERVRRRLLHAVDRRLLTHRVARLYTISGTIRERLRRFGGIPAEV
                     LYPPAPQRPYRCDGYGDELFAVSRMTPLKRMDLLLRALAEPAAQGVRCAIAGEGPDAG
                     RIHRLVSDLGLDGRVTLLGRIDTGTLVARLASCRAVVFPTYDEDYGLVTVEAFASRKA
                     VVTCTDSGGPAELVQDGVSGFVCAPTPAALAVPLARLAGERGLAERLGEGGFRTGAEI
                     TWPRTLERLILP"
     gene            4389..>4785
                     /locus_tag="PLT35_08045"
     CDS             4389..>4785
                     /locus_tag="PLT35_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29217.1"
                     /translation="MPYESTLEARIARKVTRAIVEYDLIANGDRVMVALSGGKDSWAL
                     LQILDVLRRRAPVTFDLFAVTVDTGFEGLRTDLLSETCAARGWEHHVERTSIGAAIGR
                     LLKPGDTPCSLCARFRRGVLYRLAGGLGAT"
BASE COUNT          608 a         1704 c         1689 g          784 t
ORIGIN      
        1 atgctgacgc gtcggtgccg gtggcggcgc aggggcgccg ctcgaggggc gtcgcggtgg
       61 gagaaggagc atggctggga gcgggcgcca agatcctcga tggggtccgg atcgggagcc
      121 acgccatcat cggggccggc gcgatcgtca gcgtcgatgt gccggagtac gccgtcgccg
      181 tcgggcgcac ggcgcgcatc gccgggagcc ggcgcgagga tgcgggccct gcggagtagc
      241 atgagcgcgc gcaagctcaa catcctgcac gtctgcgatc acctcggatg ggagggatcg
      301 cgcatgcacg gcgtgaagcg gctcttctcg tggatgatgc cccgcttcga tccggaccgc
      361 tatgccgtgt cgctcgtgag cctccgcagg aaggacctgt cggaggagac cctcgagtcg
      421 ctcggcgtcg acatctcgta cctccaccgg tcgaagttcg accccgcgac gctcacggcc
      481 cttctgaaag tcatcgaccg caaggccatc gacgtcctgc acctgcacgg gtacggcgcg
      541 acgaccttcg ggagggccgc cgcggccctg cggcgcctgc ccgtcgtcct gcacgagcac
      601 gcgaacctca ccgatacgcc gtggttccag aaagttgcgg accgcgcgct cgcgcccttc
      661 accgacatcg ccatcgcggt gtcgaggagc acggccgagt tcctcgtccg ggcgcgcctc
      721 gtccccgagc gcaaggtgaa agtggtgtac ctcggcgccc cgttcgacga gttcagccgg
      781 gcgcggacgg ccctggaaat cgccgcggcc cgtgaggccc tcggcatcaa ggacggcgac
      841 ttcgccgtcg gcacggtgac gcggctgcac gactcgaagg gcaaccagta cctcgtggac
      901 gcggcgcggc tggtgctcga cgcgcgcccg caggcgcggt tcgtccttgt cggcgaaggg
      961 ccgctgcggc cggatctcga ggcgcaggcg gcgcgcctcg gcctcggcga tcgcttcgtg
     1021 ttcgccggct tcgccaggga cgtcgcggcg gcgctctccg cgttcgacct gagcgtgttc
     1081 ccgtcgctct gggaaggcac gccgctcacg gcgttcgagg ctctcgcggc cggaaaggcg
     1141 atcgtggcca ccgacgcgga cggcctgctg gacgtcctgc gggacggccg cgacgcccgc
     1201 atcgtgccga agcgggacgc ccgggcgctg gccgaccgga tcatcgaatt gatggatgcg
     1261 cccgacgagc gccgccgcct cggccagcat gccaggacga ccggcgccga gtacgacatc
     1321 gcggccttcg tccgcaagat ggagcggctc tacgcgctcc tgcacgacgt gtcgcgccgg
     1381 actcgcagaa agggcgtgct ggacgaggat ctgtcgtttc tcgagaggag ccgcgcgtga
     1441 cggatcggac ggccgcggcc cgccgcccgg acgcgcgccc cggcctgctg gcggcggcgg
     1501 ctgtcgtgtg cgtgtacggc gccgtcgccc tgtcggtgga cttcccgcgc gccgccttcg
     1561 gcatccagag cgacgaggcg acctactaca tgatggggca cagcctcgtg aacgacggcg
     1621 atctggcgta ccggcgcgag gacctcgcgc gggtgtggcg cgaattcccg tcgggtccgt
     1681 ccggcgtctt cctgaagcag gggcgcgacc tcgacgtgtc gctgtccgcc gctccgccct
     1741 tcgtgcgcat cgacagcagg cccgacccgg accgggcgcg tctgttctac ggcaagtcgt
     1801 acatctatcc gctggcggcg tccccgctcg tcgccctgtt cggcacgaac ggcttcctgg
     1861 cattccacgc gctgctgctg gggctcgtga ccctggcggg gtacgtgttc ctcaacgccc
     1921 gctcgtcgcc ggctgtctcg ctgctgctgg cgctggggtt cctcctggcg tcggtcgtac
     1981 cggcctactt cgtctggatc actcccgaac tgttcaactt cagcctcgtc ttcctcggct
     2041 acttctgctg gctctacaag gaagtcgctc ccgccgggac ggtctcgcga ggcgcgcgct
     2101 ggctgctgac cccctcttcg gacctcgccg ccgccgcgct catcggcgtg gcgacattct
     2161 cgaagccgtc gaatctgctg ctcgtgctcc ctctgctgat ctggctggcg gcgcggcgcg
     2221 agtggcggcg catggcggcg gccggcttgg tcttcgccgc cgtgggcgcg gcgctcctcg
     2281 cggggaacgt cgccgtcacc ggcgactgga acttccaggg cggcgaccgg cggacgttct
     2341 acgggtcgtt cccgctgcag gagcgggatc tcgcgtggaa cgacgtcgga cagggccgcg
     2401 cgacggatcg cgtgctgtcg gaagtcatct tcgatccgcg ggtgttctgg accgtgctcg
     2461 gacacaacct cggatacgtc ttcgccggcc gctactcggg cctcgtgccg tacttctttc
     2521 cgggcgtctt cgccctcgcc gccttcctct tcgccagagg caggcgcgcg ggatggcagt
     2581 ggctggtgct gggtgccgcc gcggccgaga ttctgctgct gctcgtctgg gtgccctaca
     2641 cctacaacgg cggcggaggg tcgatcggca accgctactt cgtcaacacc tacgggacgc
     2701 tgctgttcct gctgccgccc atcgcggggg cgggcaccgc cctggtgccc tggctggtcg
     2761 gctgcctgtt ctcggcgcag atcacgttca atccgttttt cgcgtcgttc aacccgggcg
     2821 acccggccaa gcacgggccc ctgcgctggc tgcccgtcga actgactctc gtgaacgatc
     2881 tgcccatcaa cacccaggcg aaccgcgtcc gcatctggtt cggcgaggcg cgccggttcc
     2941 agatctactt cctcgacgac aacacctacg ggcgcgaggg cctcagcttc tgggtgcgcg
     3001 ggcgcgcgcg gacggagatg ctcgtgaaga cggtcgagcc cgcctcggcg ctcgacctcg
     3061 acttggccgc cggggatgcg gcgacgtcgg tgacggtgac gcgggggagg ctgtcgcggc
     3121 gcgtcgacct ggcgcccggc gccagcgcgc gcgtcagcct gccgctcgac gagggctttc
     3181 cgtacagcgg cacccgcgtg tggcgcgtgt cgatccggag cagcggcggg ttcgtgccgc
     3241 acttcctcac gccgggttct gaggacaaga ggttcctggg tgttcgcgtc acgccggagc
     3301 ttcggcccta gaggcaccgc atggagcgga ggcgatgaag atcggcgtga tcatttcgag
     3361 cccgccgctc gccgaaggcg ggcatctcgt cctggccagg tcgctcgtcg aggccctgcg
     3421 ggacgcgggg cacgaggccg gccttgtcgt gacgccccag aaccgcttcg gccgcctgcc
     3481 atcgtcatac ctcgccgcct ggctcaccga tgtcgaggtt gcctatgacg gcgccccgat
     3541 ccaccaggtg atctcgctcc ggttcccggc gtacgccgtc cgccatcccc gccacgtggt
     3601 ctggctgaat caccggatgc gcgagtacta cgacctctgg gagcggttcc gggcgaccct
     3661 ctcctggcgg gcccgcctca aggagcgcgt gcgacggcgc ctgctgcacg ccgttgatcg
     3721 ccggctgctg acgcaccgcg tcgcgaggct ctacaccatt tccggcacga tcagggagcg
     3781 gctgcggcgg ttcggcggca ttcccgccga ggtcctgtac cctccggccc cgcagcggcc
     3841 gtaccggtgc gacggatacg gcgacgagct gttcgccgtc tcgaggatga cgccgctgaa
     3901 gcggatggac ctgctcctgc gcgcgctggc cgagccggcg gcgcaggggg tgcgatgcgc
     3961 catcgcgggc gaaggcccgg acgccggccg catccatcgc ctcgtctcgg atctcggcct
     4021 cgacggacgg gtgaccctgc ttgggcgaat cgacaccggc acgctcgtcg cgcgcctggc
     4081 atcgtgccgg gcggtggtct ttcccaccta cgacgaggac tacggactgg tcacggtgga
     4141 ggccttcgcc tcgcgcaagg ccgtcgtgac ctgcaccgac agcggcggcc cggccgaact
     4201 ggttcaagac ggcgtgtccg gcttcgtctg cgcgcccaca cccgccgcgc tggccgtgcc
     4261 gctggcgcgc ctggccggcg agcgcgggct ggccgagcgc cttggcgagg gcggcttcag
     4321 gacgggtgcc gagatcactt ggcctcggac cctcgagcgg ctcattctcc cgtgagcagc
     4381 ggtcgcccat gccgtacgaa agcacgctcg aagcccgcat cgcccgcaaa gtgacgcggg
     4441 ccatcgtcga gtacgacctc attgccaacg gcgaccgcgt gatggtggcc ctgtcgggcg
     4501 ggaaggacag ctgggcgctc ctgcagatcc tcgacgtgct gcgccggcgc gcgcccgtca
     4561 cgttcgacct gttcgcggtg accgtcgata ccgggttcga gggcctgcgc acggacctcc
     4621 tcagcgagac gtgcgcggcg cgcggctggg aacaccacgt ggagcgcacc tccatcggcg
     4681 ccgccatcgg ccgcctgctg aagccgggcg acacgccctg ctcgctctgc gcgcgcttcc
     4741 gtcgaggcgt gctctaccgg ctggcgggcg gccttggcgc gacga
//
LOCUS       DAOSIF010000212         4777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15859_length_4777_cov_3.198221, whole genome shotgun sequence.
ACCESSION   DAOSIF010000212 DAOSIF010000000
VERSION     DAOSIF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15859_length_4777_cov_3.198221"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>296)
                     /locus_tag="PLT35_08050"
     CDS             complement(<1..>296)
                     /locus_tag="PLT35_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29218.1"
                     /translation="QPPLPLPSGGAGNGNIYVRAKQVATGFTFARSGSSLLEGRFGWS
                     RTEAGKNPPGLGSEPIQSMYGITGLPSDARIAGGLNTQLLTGWSSYGRQETNPQ"
     assembly_gap    297..306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<307..1560)
                     /locus_tag="PLT35_08055"
     CDS             complement(<307..1560)
                     /locus_tag="PLT35_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG29219.1"
                     /translation="MSSHRYALIVCAAAACLLASALPSSAQFDTATVVGTVKDNSGGV
                     VPGATVTLTNLDTGVTSVHTTDGTGSYEFVTVRIGRYKVTAELQGFSTAVADGFQVTV
                     GARQRVDLQLSPGQVTETVQVTGAAAVLETDSSQRAHLITSRQAVELPLNTREYSALA
                     LLTPGVKQSSLNQGSGTNNREGSFNVNGMRSTFNNFLLDGLDNNSYGTSNQGFSNQVI
                     QPSPDAVAEFKVVTNNMSAEYGRAGGATVNVAYKSGTNRYTGAAWEFYRDTALNATGF
                     FKPTSGVKPTLEKNQFGGTFGGPIIRNKAFFFADAEVWRQTRKQVAFQTIPNPTERQG
                     ILKVDVRNPQTGDVYPAGTPIPMTAFARKVLSGLPDPTRPELVGNNYDLLQNFEYNTY
                     KYGGKVDYQASERLSMFARFGYRNVD"
     gene            1972..>4777
                     /locus_tag="PLT35_08060"
     CDS             1972..>4777
                     /locus_tag="PLT35_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29220.1"
                     /translation="MRKVFVDPVVLPDARFRDGLGIRYVRLEGADEVVEVFRPLDDLA
                     VHRTAIQQRVNRLTSFRQVRFVPLRRADVVHDSAGTLEILSDFIPGHRLSDVLEAAAA
                     GAIAITACAAIHIVREVLGALAVLAESRGLTHGAVGPERLLLVPKGRVLVADYVLGGA
                     LAQLEYSRGRLWREFRVAAAPGKGAPKLDMASEVVQVAVTAVALLRGRPVEAEEYPDG
                     LLDLVASLGRSRSSSRGEAVPAALLTWLRRALHLDAGSRLTSVSEARLALEKVLSKQA
                     VASGGAAAVRALADDYGRLTGVVDAASAARAAAEAAKRTERERDATVGMPPERSSDAA
                     GPQTAARTVLMPAFTLLPDATIDADGEVVAGASFVEHAGDSGQTDPPVGWRVPERASP
                     DIATVVGPLLDRTATPRLPRDAEFPEEIIDLDALTESEGAATAPVPAPMSPPPIEAGR
                     SGADSSVGDERAAPRTLDIAALEAEPEIAACFLNEEDRPVVRDLTPVDTMPALTPLPL
                     QEIELSVVETEPAVAALFQPEPIAGAAREAAPMEEPAPLAQPEAVAVLAIESEAAVEA
                     PPIEAGRSADESSVGDERAAPRTLDIAALEAEPEIAACFLNEEDRPVVRDLTPVDTMP
                     ALTPLPLQEIELSVVETEPAVAALFQPEPIAGAAREAAPVEEPAPLAQPEAVTVLAVE
                     SEAAVEQALVVEVLGSSMADSFRSEASILVVPDPNAGNAAAAANADSAPGSEQALPAP
                     SKPDALPRLPSDWLIDLDVASRRRHRTPEPERPALGVTIELPDLVGSFAAGASQSDVP
                     PPPLTPRRRLGVAPLDAPGVAEEQIEVAAAAHQALPQEGLPPVPLARVGPQFDGAGDS
                     ATDVEQSPAEATEGEAGPVFPRVAPSVRRVREEARRRRLARARAALAGSAVLAARAAH
                     AAGAAMVSAGTSAGRALRAAW"
BASE COUNT          643 a         1672 c         1716 g          736 t
ORIGIN      
        1 cactgcgggt tcgtctcctg gcgcccgtag gacgaccagc cggtcagcag ctgcgtgttc
       61 aggccgccgg cgatccgcgc atcgctcggg agcccggtga tgccgtacat gctctggatg
      121 ggctcggagc cgagccccgg agggttcttg cccgcctcgg tcctcgacca gccaaaccgg
      181 ccttcgagga gcgacgagcc cgagcgcgcg aacgtgaacc cggtggccac ctgcttggcc
      241 cgcacgtaaa tgttgccgtt gcccgcgccg ccagacggca gcggcagcgg cggctgnnnn
      301 nnnnnngtcc acgttgcggt agccgaagcg cgcgaacatc gacaggcgct cgctggcctg
      361 gtagtccacc ttgccgccgt acttgtaggt gttgtactcg aagttctgca gcaggtcgta
      421 gttgttgccg accagttcgg gccgtgtcgg atccggcagc ccgctcagga ccttcctcgc
      481 gaacgccgtc atcggaatcg gcgtcccggc ggggtacacg tcccccgtct gcgggttccg
      541 cacgtccacc ttcaggatgc cctgccgctc ggtcgggttc gggatcgtct ggaacgccac
      601 ctgcttccgg gtctggcgcc acacctcagc atcggcgaag aagaacgcct tgttccggat
      661 gatgggaccg ccgaacgtcc cgccgaactg gttcttctcc agcgtcggct tcacgccgct
      721 cgtcggcttg aagaaccccg tggcgttcag cgcagtgtca cggtagaact cccaggccgc
      781 gccggtgtac cggttggtgc cgctcttgta ggccacgttg accgttgcgc ccccggcccg
      841 cccgtactcc gcgctcatgt tgttcgtgac gaccttgaac tcggccaccg cgtcgggcga
      901 cggctggatg acctgattcg agaacccctg gttgctcgtg ccgtacgagt tgttgtcgag
      961 gccgtccagc aggaagttgt tgaacgtgct ccgcatgccg ttgacgttga acgatccttc
     1021 ccggttgttc gtgccgcttc cctggttcag gctcgactgc ttcacgcccg gcgtcagcag
     1081 ggcgagcgcc gagtactcgc gggtgttcag cgggagctcg accgcctgcc tcgacgtaat
     1141 caggtgggcg cgctggctcg agtcggtctc caggactgcc gccgcgccgg tgacctgcac
     1201 cgtctcggtc acctggccgg ggctgagctg cagatcgacg cgctggcggg cgccgacggt
     1261 gacctggaac ccgtcggcca cggcggttga gaaaccctgc agttcagctg tcaccttgta
     1321 gcgcccgatg cgcaccgtga cgaactcgta actccccgtg ccgtccgtgg tgtggacgga
     1381 ggtcacgccg gtatcgaggt tcgtgagcgt gacggtagct ccaggcacga cgcctccgga
     1441 gttgtccttc acggttccca cgacggtggc cgtgtcgaac tgcgcggagg atgggagcgc
     1501 ggatgccagc aggcacgccg ccgcggcgca gactatgagt gcgtagcggt gtgaagacac
     1561 gtgaagatcc ctcccggatg agtgctgtcg actgtatgac gcgcgtggac cgcaacggac
     1621 gcggaagcct agctccgctt ccgtgcgatg tcaaggccgg gcgcgagcct cggcggcggc
     1681 gagccgtcgc ctcgatcgcc gcacgctgga agcgccgcct cccgcccatt cagccgcctg
     1741 gccgccgcgc ccggcgggct gccggggcgc cttggggcga acgctcgagt ccggccaacg
     1801 cgcacagagc cggcatcggc ctcagggcag cgtcgaaccg gacggcctgt gtgcggaccg
     1861 gtcgtccggg ccccctagtg gagggcgccg ccgacccgat aagacggaac agggagtctc
     1921 cgcgccaccc gctgcagagg cggcgcggac gttgcaaagc ggttctggcc catgcgaaag
     1981 gtgttcgtcg atccggtcgt gctgccggac gcgcgcttcc gggacggcct cggaatccgc
     2041 tacgtccgtc tggagggcgc ggacgaggtg gtcgaggttt tcaggccgct cgatgacctc
     2101 gccgtgcacc ggacggcgat ccagcagcgc gtcaaccggt tgacgtcgtt ccggcaggtc
     2161 aggttcgtgc cgctcaggcg cgccgacgtc gttcacgaca gcgccggcac tctcgagatt
     2221 ctctcggact tcattccggg gcaccggctc tcggacgtgc tggaggccgc cgccgccggc
     2281 gcgattgcga tcacggcctg cgccgccatc catatcgtgc gtgaagtgct cggggccctg
     2341 gcggtgcttg ccgagtcgcg cgggcttacg cacggcgccg tcggcccgga acgcctgctg
     2401 ctcgtgccca aagggcgcgt tctcgttgcc gactacgtcc tcggcggtgc gctggcccaa
     2461 ctcgagtaca gccgcggcag gctctggcgg gagttcaggg tggccgctgc tccggggaag
     2521 ggcgctccca agctcgacat ggcgtccgag gtcgtgcagg tggcagtcac ggccgttgcg
     2581 ctgctgaggg gcaggcccgt cgaggccgag gagtaccccg atggtcttct cgatcttgtc
     2641 gcctcgctcg gccgctcgag gagcagcagc cgcggcgaag ccgtcccggc ggccctgctc
     2701 acctggctca ggcgggcgct gcatctcgac gcgggcagcc gcctgacgag cgtctccgaa
     2761 gcgcggctgg ccctcgagaa agtgctctcg aagcaggcgg tggcgtctgg cggggcagct
     2821 gccgtcaggg ccctggccga tgactacggc cgcctgaccg gcgtggtcga tgccgccagc
     2881 gcagcgcgag ccgcagccga agccgcgaag cggaccgagc gggagcgcga cgcaacggtc
     2941 gggatgccgc ccgagcggtc ctccgatgcc gccggtccgc agacagccgc ccgaaccgtc
     3001 ctcatgcctg cgttcacgct gctgccggat gcgacaatcg acgcagatgg ggaagtggtg
     3061 gctggcgcct cgtttgtcga acacgccggc gacagcgggc agacggaccc gcccgtgggc
     3121 tggcgcgtgc cggaacgggc ctcaccagat atcgcgaccg tcgtcggccc gcttctcgac
     3181 cggacggcca cgccgcggct cccgcgcgat gcggaattcc ccgaggagat catcgatctc
     3241 gacgcactca ccgagtcaga aggcgccgcg acggccccag tccctgctcc aatgtccccg
     3301 ccgcccatcg aggcgggacg ctccggcgcc gactcgagcg ttggggacga gcgcgcggcg
     3361 ccgcgcacgc tggacatcgc ggcgctggag gcggaacccg agattgccgc ctgcttcctg
     3421 aacgaagagg atcggccggt tgtccgggac ctcacgcccg tcgacacgat gccagcgctg
     3481 acgccgttgc cgctgcagga gatcgagctc tcggtggtgg agaccgagcc ggccgtggcg
     3541 gcgctcttcc agccggagcc gattgccggc gccgcgcgcg aggccgcgcc gatggaggag
     3601 ccggcgcccc tcgcgcagcc cgaggccgtc gccgtcctcg ccatcgagtc cgaggccgca
     3661 gtcgaggcgc cgcccatcga ggcgggacgc tccgccgacg agtcgagcgt gggggacgag
     3721 cgcgcggcgc cgcgcacgct ggacatcgcg gcgctggagg cggaacccga gattgccgcc
     3781 tgcttcctga acgaagagga tcggccggtt gtccgggacc tcacgcccgt cgacacgatg
     3841 ccagcgctga cgccgttgcc gctgcaggag atcgagctct cggtggtgga gaccgagccg
     3901 gccgtggcgg cgctcttcca gccggagccg attgccggcg ccgcgcgcga ggccgcgccg
     3961 gtggaggagc cggcgcccct cgcgcagccc gaggccgtca ccgttctcgc cgtcgagtcc
     4021 gaagccgcgg tcgagcaggc cctggttgtt gaagtgctcg gcagctcgat ggccgactcg
     4081 ttccgcagtg aagcgtcgat cctggttgtg ccagacccga acgccgggaa cgccgctgcc
     4141 gccgcgaatg ccgattccgc gccgggaagt gagcaggctc ttcccgcacc ctccaagccg
     4201 gacgcgctgc cgcggctgcc gtccgactgg ctgatcgatc tggatgtcgc ctcgcggcgc
     4261 cggcaccgca cgcctgagcc ggagcggcca gcgctcggcg tgacgatcga gcttcccgat
     4321 ctggtgggtt ctttcgccgc cggtgcgagc cagtccgacg tgccgccgcc gcccttgact
     4381 cctcgcagac gcctcggcgt cgcgcccctc gacgcgccag gcgtcgcgga ggagcagatc
     4441 gaagtggcgg cggccgcgca ccaggcgttg ccgcaggagg ggctcccgcc ggtgccgctg
     4501 gcccgggtcg gcccccagtt cgatggtgca ggggactcgg cgaccgatgt cgagcagtcc
     4561 ccggcggagg cgacggaggg cgaggcaggc ccggtgtttc cccgtgtggc ccccagcgtg
     4621 cggcgtgtgc gcgaggaggc caggcgccgg cggctggcgc gcgcgcgcgc cgcgcttgcc
     4681 ggctcggcag tactggcggc aagggccgcg cacgccgcgg gggccgccat ggtttccgct
     4741 ggtacgtcgg cgggccgcgc gctgcgggcc gcgtggg
//
LOCUS       DAOSIF010000213         4771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15890_length_4771_cov_3.893130, whole genome shotgun sequence.
ACCESSION   DAOSIF010000213 DAOSIF010000000
VERSION     DAOSIF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15890_length_4771_cov_3.893130"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLT35_08065"
     CDS             <1..663
                     /locus_tag="PLT35_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29221.1"
                     /translation="LSAALAASRAAVTNYLATASLDTGLALGAAVTLSLRAEAILSLL
                     AAITPATTHAGTREASLGATAALAPAGRLDVAGMAALASVASLTPATRLDLAVTAALD
                     ILAALVASRGASTAYLASASLDIAAAQTATTTLAAHVQAALAILGQLQPASQASASVT
                     AVLAATIALQASRRPDLTTALIWMLAEAILAGAVTSELLTGGTLTDGDITAGGLTNED
                     LQ"
     gene            674..1180
                     /locus_tag="PLT35_08070"
     CDS             674..1180
                     /locus_tag="PLT35_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29222.1"
                     /translation="MKTTLGTVQMETRYEAVCRDASGAVKWIAHVTNLVVTAGLNKLL
                     DATFKTGLTTPAWYVGLKGTGTVAAGDTMASHAGWTELTAYSESVRQTFTPGTISGGS
                     VNNSGSKAVFTANGSMTVLGLFLADNSTKGGTSGTLYGAGDFSEGSRAMVSGDTLTVT
                     CTLTAAAA"
     gene            1192..1584
                     /locus_tag="PLT35_08075"
     CDS             1192..1584
                     /locus_tag="PLT35_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29223.1"
                     /translation="MADLATMNERASGRYIGTLRDEAGTLLTAADLTAATLTLYDQAS
                     GTIVNSRSAQNVKNANGVTLYDTLQTDVATGKTYNLLWLIEPADNAIVGTGPAERRVA
                     LFVFTCSATKEVPHEVTWIVRNLTKRPA"
     gene            1634..1975
                     /locus_tag="PLT35_08080"
     CDS             1634..1975
                     /locus_tag="PLT35_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29224.1"
                     /translation="MPDEYTNGNGTRNGRTDPLAGLPWWVRAVFLVGVPSAIALGLVW
                     SDRAQLAEQIHTNGVLLRQIDADARAHDARVVLRFQELSTATSETNRILTASCVNAAK
                     TAEARERCIGR"
     gene            2149..2595
                     /locus_tag="PLT35_08085"
     CDS             2149..2595
                     /locus_tag="PLT35_08085"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HOG29225.1"
                     /translation="MTGPSPHLSWDELACHDAIRTPYPLDWRSDPTRLRALCDAFEAV
                     RAECSLEAGMPCPIIVSSGYRTPEYQAKLAAIPTFRAAQRSQHVQGRALDLVCPRMLT
                     WDQFENAVKRAASRAGSPIRYIEYRPSRQYIHIDVRPTKHLVQETV"
     gene            2592..3596
                     /locus_tag="PLT35_08090"
     CDS             2592..3596
                     /locus_tag="PLT35_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29226.1"
                     /translation="MSSLFSPHRQYEVKIQDDGNLVIYTASGRVVWASGADPSPDPVP
                     PAPVVPPPTDTWPRLGASYYTSLTDPRIDPPTFAVRLADLGVTLTRAWLFDAWAIGAS
                     AGTGCYDGYLPWVRGADGRFDLWSMDAAYLDRLRAYVEAMAAQGILVQLCGCELYTWS
                     DRKQGLLWVPPSARQPWRCNSQGVDYHDDHAFERIGQPMGRDAFLGACYQRVVATLDG
                     LPYSVELGNELPEKTLHARLAVAWRDAGYRGSISVNRQEDTPGQYRNMRIGQMGGYDR
                     IAFHGKRSLAYLDEEFPREPELRTFQLFWASGPDPSRVILSSDGCRASTDVARLSYSW
                     "
     gene            complement(3866..3941)
                     /locus_tag="PLT35_08095"
     tRNA            complement(3866..3941)
                     /locus_tag="PLT35_08095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3906..3908),aa:Lys,seq:ctt)
     gene            complement(4198..>4771)
                     /locus_tag="PLT35_08100"
     CDS             complement(4198..>4771)
                     /locus_tag="PLT35_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29227.1"
                     /translation="PAFGLIVAGGACLAYASAAAIHGVTWLVQERGFRFESAAYTAGG
                     VAVAAGLCGNLAGGWVADWCARRWRGGRGWSLVLLTLFFAPFSVAFYLLPPSSALFYV
                     CWIVSSASTVAYFGPVFSSIQEVAPPQVGSSAVAFGLLVVNIAGVGSGSWLTGVIGDG
                     AGLTAGLLASVGVTLVGVPAFLLAARRWPR"
BASE COUNT          807 a         1729 c         1618 g          617 t
ORIGIN      
        1 ctctcggcgg ccctggcggc ctcccgcgcg gccgtcacga actatctcgc gaccgcctcg
       61 ctcgacacgg gcctcgcgct cggggcggcc gtgacgctct cgctgcgcgc cgaggcgatc
      121 ctgtcgctgc tcgccgccat cacgcccgcc acgacccatg ccggcacacg cgaggcgtcg
      181 ctcggcgcga cggcggccct cgcgccggcg ggccgcctgg acgtggcggg gatggccgcc
      241 ctggcgtccg tcgcgagcct cacccccgcg acccggctcg acctggcggt caccgcggcc
      301 ctcgacatcc tggccgccct ggtcgcctcg cgcggggcgt ccacggccta tctcgcgtcg
      361 gcgagtctag acatcgccgc cgcgcagacc gcgaccacga cgctcgccgc gcacgtccag
      421 gcggcgctcg ccatcctcgg gcaactgcag ccggcgtcgc aggcatcggc gtccgtgacg
      481 gcggtcctgg ccgccacgat cgcgctccag gcctcccggc gtccggatct cacgaccgcg
      541 ctcatctgga tgctagcgga ggcgatcctc gccggcgccg tgacctccga gctgctcaca
      601 ggcggcacgc tcaccgacgg cgacatcacc gcaggcgggc tcacgaacga agacctgcaa
      661 tgaaggagag accatgaaga cgaccctcgg cacggtgcag atggagacgc gctacgaagc
      721 ggtctgccgc gacgcgtcgg gcgcggtcaa gtggatcgcg catgtcacga acctcgtggt
      781 gacggcgggg ctcaacaagc tcctcgacgc cacgttcaag acggggctca ccacgccggc
      841 gtggtatgtc ggcctcaagg gcaccggcac cgtcgcggcg ggcgatacga tggcctcgca
      901 cgccggctgg acggagctca ccgcctacag cgagagcgtg cggcagacct tcacgcccgg
      961 caccatctct ggcggcagcg tcaacaacag cggctccaaa gccgtcttca ccgccaacgg
     1021 ctcaatgacg gtgctcggcc tgttcctggc cgacaacagc acgaagggcg gcacgagcgg
     1081 caccctgtac ggcgcaggcg acttcagtga gggctcgcgc gcgatggtct caggggacac
     1141 cctcaccgtc acctgcacac tgaccgccgc ggccgcataa ggaggctcgg gatggcggac
     1201 ctcgcgacga tgaacgagcg ggcgagcggc cgctacatcg gcacactgcg cgatgaggcc
     1261 ggcacgctgc tcacggcggc cgacctcacc gcggccacgc tcacgctgta cgaccaggcc
     1321 agcggcacca tcgtgaacag ccggagcgcg cagaacgtga agaatgccaa cggcgtcacg
     1381 ctctacgaca cgctgcagac ggacgtggcc acgggcaaga cctacaacct gctctggctc
     1441 atcgagccgg cggacaacgc catcgtcggc acggggccgg cggagcggcg cgtggcgctc
     1501 tttgtgttca cctgtagcgc gacgaaggaa gtcccgcacg aggtgacctg gatcgtccgg
     1561 aacttgacca agcggcccgc gtaagtccgc ctagaccagc gcggcaggca cacggtcgcg
     1621 cggagacacg acgatgcctg acgagtacac gaacggcaac gggacgcgga acgggcgcac
     1681 tgatccgctg gcgggcctgc cttggtgggt gcgcgcggtg ttcctggtcg gggtgccctc
     1741 ggcgatcgcc ctcggcctgg tctggagcga ccgcgcccag ctcgcggagc agatccatac
     1801 caacggcgtg ctcttgcggc aaattgacgc cgacgcgcgc gcccacgacg cgcgcgtggt
     1861 gctccgcttc caggagctca gcacggcgac ctcggagacg aatcgcattc tcactgcgtc
     1921 atgcgtgaac gcggcgaaga cggcagaagc gcgggaacgc tgcatcggac gatgatgacc
     1981 taacccagag accgcgtctg cagaggcccg cctgatcagc gggctgctcc tggcctggcc
     2041 ggccaggcgg gcgtgatcac aggccggaac gaccaggcca cgttcctgtt cgcccacagt
     2101 cgggcggatc ggacgtggcc tgtgtcgtct ccggtggagc aaccgggcat gactggaccg
     2161 agtccgcacc tgtcgtggga cgagctcgcg tgccacgacg cgatccgcac gccgtatccg
     2221 ctcgactggc ggagcgaccc gacgcgactc agggcgctct gcgacgcttt cgaggcggtg
     2281 cgcgcggagt gctcgctcga ggccgggatg ccgtgtccca tcatcgtcag ttccgggtac
     2341 cgcacgccgg agtatcaggc gaagctggcg gccatcccca cgttcagagc ggcgcagcgc
     2401 agccagcacg tccagggccg ggccctggac ctcgtgtgtc cgcggatgct cacctgggat
     2461 caattcgaaa acgccgtgaa gcgcgcggcc tcaagagccg ggtcaccgat ccgctacatc
     2521 gagtaccgcc cgtcgcggca gtacatccac atcgacgtcc ggccgacgaa gcacttggtg
     2581 caggagacgg tatgagcagc ctcttcagcc cgcaccggca gtacgaagtc aagatccagg
     2641 acgatgggaa cctcgtcatc tacacggcga gcgggcgggt cgtgtgggcc tctggcgcgg
     2701 acccatcgcc agaccccgtt ccgccagcgc ctgtcgtgcc gccgcccacc gacacctggc
     2761 cgcgcctagg cgcgagctac tacaccagcc tgaccgaccc ccgcatcgac ccgccgacgt
     2821 tcgccgtgcg tctcgccgac ctcggcgtga cgcttacgcg cgcgtggctg ttcgacgcct
     2881 gggccatcgg cgcctcagcc ggcacgggct gttacgacgg gtatctgccg tgggtgcgtg
     2941 gcgcagacgg gcgattcgac ctctggtcga tggatgcggc ctatctcgac cggctgcgcg
     3001 cgtacgtcga ggcgatggcg gcgcagggca tcctcgtgca actctgcgga tgcgagcttt
     3061 acacgtggag cgaccgcaaa cagggcttgc tctgggtgcc acccagcgcc cgccaaccct
     3121 ggcggtgcaa cagccagggc gttgactatc acgacgacca cgccttcgag cgaatcggac
     3181 agccgatggg ccgcgatgcg ttccttggcg cgtgctatca gcgggtggtc gccacgctcg
     3241 atggcctgcc gtacagcgtg gagctgggca acgagctgcc ggaaaagacc ctccacgcga
     3301 gactcgcggt cgcctggcgc gacgcgggct accgcgggtc gatcagcgtg aaccgccagg
     3361 aggacacccc gggccagtac cgcaacatgc ggatcgggca gatgggcggc tacgaccgga
     3421 tcgccttcca cggaaagcgg tcgctcgcct acctcgacga ggagttcccg cgcgagcccg
     3481 agctccgcac attccagctc ttctgggcca gtgggcctga cccgtcgcgg gtcatcttgt
     3541 cgtctgacgg ctgccgcgcc tcgaccgacg tcgcgcggct cagctactcg tggtgagggg
     3601 cgcgtctcag gcgcggcact cagggtggga gctcggggcc ccggtgtccg gccgggagcc
     3661 ccgagaggag ccaggatgtc gctggcccca gtggcgccac gcccagtgta ccatccgtgc
     3721 cagcctcggt gccagccacg cctgagacac gctggcgaaa ggtggccact tctggccact
     3781 tctggcacat gagcccaggc aaggcctgtg gccgtcattc aggcgaaact agcgcaattc
     3841 ctcaggaaaa caggggcgaa agaagtggtg agccgcgtag gaatcgaacc tacaacccgc
     3901 agattaagag tctgctgctc tgccaattga gctagcggcc cactcccctg cactccgccg
     3961 gcggcggacc cggctgcgga agccggaatg agacagtata gcgcgcaacc gggatcaggt
     4021 ccaagatcgc gacgccaacc gcaatccggg ccgcgtctcc cgcgcctccg gaccgcagga
     4081 cgtgaggctc cacctgccgg gctgccagac ggcgccggcg agcgccgcac ggcgcgccgc
     4141 gcggccggac cccgcgccca ttcggccggc ggcgcttcga acgatcctgc cgcccgttca
     4201 ccgcggccag cgccgtgcgg cgagcaagaa cgcgggcacg ccgacgagcg tcacgccgac
     4261 gctcgcgagc aggccggccg tcaggcccgc gccgtcgccg atgacgccgg tgagccagga
     4321 ccccgagccc acgccggcga tattgacgac caacagcccg aaggccaccg cgctggagcc
     4381 aacctgcggc ggagccacct cctggatcga cgagaacacg gggccgaagt acgccacggt
     4441 ggatgccgag gagacgatcc agcacacgta gaacagcgcc gatgacggag gcagcaggta
     4501 gaacgccacg ctgaacggcg cgaagaagag cgtgagcagc acgaggctcc agccgcgacc
     4561 gccgcgccac cgccgcgcgc accagtccgc cacccagccg cccgcgaggt tgccgcacag
     4621 cccggcggca acggcgacgc cccccgcggt gtaggccgcc gactcgaagc ggaacccccg
     4681 ctcctgcacg agccacgtca cgccgtggat ggccgcggcc gacgcgtacg cgaggcaggc
     4741 cccgccagcc acgatgaggc cgaaggcggg a
//
LOCUS       DAOSIF010000214         4757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15948_length_4757_cov_3.648873, whole genome shotgun sequence.
ACCESSION   DAOSIF010000214 DAOSIF010000000
VERSION     DAOSIF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15948_length_4757_cov_3.648873"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLT35_08105"
     CDS             <1..870
                     /locus_tag="PLT35_08105"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG29228.1"
                     /translation="QGESGTGKELIAKAIHAGSARRQGRYCAVNCAALSDDLFESELF
                     GHVRGAFTGASSDRQGLFEEADGGTLFLDEIAELSPRAQAKLLRAIQEGEIRRIGENR
                     ARRVDARIVSASNRSLADEAREGRFRTDLLFRLDVIRISVPPLRERREDIVPLARRFW
                     REATGRVGVRAELTHAALAALARYDWPGNVRELQNTLAALAVHAPPRGRVGPASLPQA
                     VSEAAAGLPDTAVTLAVARRRFEEGYVRTALARVGGCRSEAAAALGLTRQGLAKLMGR
                     LGIDAGAGGDAAY"
     gene            complement(864..2243)
                     /locus_tag="PLT35_08110"
     CDS             complement(864..2243)
                     /locus_tag="PLT35_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29229.1"
                     /translation="MKRTLALALALTALAAGLIVAGLAMQRDIRYRELVAAGNSALES
                     DRPFEAIEAFSGAIALKERSMIAHLRRGEAYRRRGESEAALRDLRAAARIDPSAVRPA
                     ELLGDLSCDLGRLPQAVDAYRKCAAIDDADPRVQYKLGLALFRAGDAAGAVEPLRRAA
                     ALAPRVSESHYLLGLCLKHTRDTDGAIAAFEQAIAGSPALLAAREELAGVYAEKGRLD
                     DAAAQLEAIAALEPDRPERQAALALALARAGRQDQAVGLLGQAAQRFPDHAVVFSALG
                     RVWFEAAARTRDRVGLRKAIEALEPVTRGAAASGDDLARYGQALVLSEDLVRGEAALR
                     AAADRLPVSPDTLIWLANAAERLRHYAIVRMALERWAAISPESDPNLPAVYERVGDLA
                     HRLGDTAAAVSAWRIAAGPSGSPALLARLAGAELAIGDIDAARKTIGRGLALDPRHPA
                     LLGLRARAQ"
     gene            2348..2974
                     /locus_tag="PLT35_08115"
     CDS             2348..2974
                     /locus_tag="PLT35_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG29230.1"
                     /translation="MPGELVVKDARALDWPGVQDEDAAFVRRCVAGENAACAALVANH
                     QRMVYQLAYHLLGDVEEARDLSQDVFLQVFRTLDRFRGQSALRSWIYRIAVNLARNRQ
                     RWWRRRLRKDQVSLDEHVERHGPDVLTDASATPEVSLRRRETAERVWRALAGLPFEQR
                     TAIVLREIDGLSYEEIAFALGIAVGTVKSRLTRARVALRERLREERAT"
     gene            2971..3756
                     /locus_tag="PLT35_08120"
     CDS             2971..3756
                     /locus_tag="PLT35_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOG29231.1"
                     /translation="MMPLSCAVVRGRLSAYQDGELPVASRVAVDAHLRACASCAAELD
                     GLRELGALLRERAADSAACRPFSLEALGAETLNRLCAERDESVPALLSRAFQDMRLGY
                     AAVGSAALTCVSVLLVIVIFHFAPLSARADSLAGMMDTLGAPAIGLDSRVVSPPSGWS
                     LISEDLVSEEDAVFALAAVVTRNGRIASLEYLSDQSTNSERERAIRLLDELSRARFEP
                     ARLGGTPVTVKTVLVLARTTVRGKMPPAAKQSRLPRGPDAVRG"
     gene            complement(3772..>4757)
                     /locus_tag="PLT35_08125"
     CDS             complement(3772..>4757)
                     /locus_tag="PLT35_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29232.1"
                     /translation="IAWRASGAGPGAIRIATRTGNTAVPDDTWSGWSAPYQRAEGDAV
                     TSPAARYLQWRAELKGQFAGSASPALASVSIAYLQRNARPRVTEITVHPPGIVFQRPY
                     PAGEPEIAGLGDVPGEARAPAFATPLGSTQQTPASGPALGRRMYQKGLQAFAWKAEDD
                     NDDRMEYAVFFRAVGDAQWKTLRANAPELLVTWDTTSVPDGTYVVKVTASDAPNNPAE
                     QALTGELESAAFDVDNSAPAITILRAVQGASSSDVEFEVADSHSPLSVVEYSIDSGRW
                     IAAYPVDGAADSRRERYAIRIEGKAEGRLVIRATDGMNNTATARVGGAGLR"
BASE COUNT          649 a         1685 c         1745 g          678 t
ORIGIN      
        1 cagggcgaga gcgggaccgg caaggaactc atcgccaagg cgattcacgc cggatcggct
       61 cggcggcaag ggaggtactg cgccgtcaat tgcgccgcgc tcagcgacga cctcttcgag
      121 agcgagctgt tcgggcacgt tcggggcgcc ttcaccggag ccagttccga ccggcagggc
      181 ctgttcgagg aagcggacgg cgggaccctc ttcctcgacg agatcgccga actctcgccc
      241 cgcgcgcagg cgaagctgct gcgcgcgatt caggagggcg agatccggag aatcggggag
      301 aaccgcgccc ggcgcgtgga cgcacgcatc gtgtcggcgt ccaatcgctc tctcgccgac
      361 gaggcgcggg aaggtcgatt ccgcaccgac ctcctgttcc gcctagatgt catccgcatc
      421 tcggtgcccc cgctgcgcga gcggcgggag gacatcgtcc ctcttgcacg ccgcttctgg
      481 cgggaggcga cggggcgcgt cggcgtgcgt gccgagctga cgcacgctgc gcttgctgcg
      541 ctcgcgcgct acgactggcc cgggaacgtg agagaactgc agaacaccct ggccgccctc
      601 gccgtccatg cgcctccgcg cggaagagtc gggccggcga gcctgccgca ggccgtcagc
      661 gaggcggccg ccggtctccc ggacacggcc gtgaccctgg cggtcgcgcg ccggaggttc
      721 gaggaaggct acgtccgtac ggcgctcgcg cgcgtgggcg gatgcaggag cgaggcggca
      781 gcggcgctcg gcttgacgcg tcagggcctc gcgaaactga tggggcggct ggggatcgac
      841 gccggggcag gaggagacgc cgcctactga gcgcgtgcgc gcaggcccag cagcgcgggg
      901 tggcgcgggt ccagggcgag cccgcgccca attgtcttcc tggcggcgtc gatgtcaccg
      961 atcgcgagtt cggcgccggc cagtcgcgcc agcaacgcgg gagaccccga cgggcccgcc
     1021 gcaatcctcc aggctgacac ggcggcagcc gtgtctccga gccgatgagc gaggtcgccc
     1081 acgcgctcgt acacggccgg gaggttcgga tcggactcgg gcgagatggc ggcccaccgc
     1141 tcgagcgcca tgcgcacgat ggcgtaatgg cgcaggcgct cggccgcgtt ggccagccag
     1201 atcagcgtgt cgggcgatac cggcagccgg tcggccgccg cccgcagcgc cgcttcgccg
     1261 cgcacgaggt cctcggagag cacaagcgcc tggccgtagc gggccaggtc atctcccgac
     1321 gccgccgcgc cccgggtgac gggttcgagg gcctcgatgg ctttgcgcag cccgacgcga
     1381 tcgcgagtgc gcgcagcggc ctcgaaccag acacgcccga gcgcggaaaa gaccaccgca
     1441 tgatcgggaa agcgctgcgc ggcctgaccc aagaggccga cggcctgatc ctgcctgccc
     1501 gcgcgcgcca gggcaagcgc gagcgcggcc tgccgctcgg gccggtcggg ctcgagcgcc
     1561 gcgatggcct ccagctgcgc ggcggcatcg tcgagccggc ccttctcggc atacacgccc
     1621 gccagttcct cgcgagcggc aaggagcgcg ggcgatcccg cgattgcctg ctcgaaggcg
     1681 gcgatagctc cgtcggtgtc cctggtgtgc ttcaggcaca gccccagcag gtaatgggac
     1741 tccgacacgc ggggagcaag cgccgcagcg cggcggagcg gttcgactgc tcccgccgcg
     1801 tcccccgcgc ggaaaagggc caggccgagc ttgtactgca cccgggggtc ggcgtcgtcg
     1861 atggcggcgc acttgcggta ggcgtccacg gcctgcggga gccgcccgag atcgcagctc
     1921 aagtccccga gcaactcggc gggccgcacc gccgaggggt cgatcctggc cgccgccctc
     1981 aagtcccgaa gcgccgcctc ggactcgccg cgcctgcggt aggcctcccc gcgccgcagg
     2041 tgggcgatca tcgagcgttc cttgagggca atggcgccgc tgaaggcctc gatggcctcg
     2101 aacggccggt cgctctccag cgcgctgttg ccggccgcta cgagctcgcg gtaccggatg
     2161 tcccgctgca tggcgagccc cgcgacgatg agcccggcgg ccagcgccgt gagcgccagc
     2221 gccagggcga gcgtgcgctt catcccggtt ctatcgtagc agcagaagga acctttgccg
     2281 catctccgtg tctaatccag agagggcgtt ggcgcgcatg cgtactgcta tagttggagg
     2341 cggaacggtg cccggagagc tggtggtgaa ggatgcgcgc gcactggact ggcctggtgt
     2401 acaggacgag gacgcggcgt tcgtgcgccg ctgcgtcgcc ggcgaaaatg cagcgtgtgc
     2461 cgccttggtc gcgaaccatc agcggatggt gtaccagctc gcctaccacc tgctggggga
     2521 cgtcgaggag gcgcgggacc tgtcgcagga cgtcttcctg caggtcttcc gcacactcga
     2581 tcggttccgc gggcagtccg ccctgcgcag ctggatctac cgcatcgccg tgaacctggc
     2641 ccggaaccgc cagcgctggt ggcgccggcg gctgcgcaag gatcaggtgt ccctcgacga
     2701 gcacgtcgag cgccatggcc ccgacgtgct gacggacgcg tcggcgacgc cggaggtgtc
     2761 gctgcggcgc cgggagacgg ccgagcgcgt ctggcgcgcg ctggcagggc tgccgttcga
     2821 gcagcggacg gccatcgtgc tgcgggaaat cgacgggctg agctacgagg agatcgcctt
     2881 cgcgctcggc attgcggtcg ggacggtgaa gtcgcgtttg acgcgcgccc gggtcgcgct
     2941 gcgcgagcgg ctgcgcgaag agagggccac atgatgccgc tgtcgtgcgc cgtagtccgg
     3001 ggccggctgt cggcctacca ggacggagaa ctgccggtcg ccagccgcgt ggccgtcgac
     3061 gcgcacctcc gcgcgtgcgc ctcctgcgcg gcggagctcg acggcctgcg tgagctgggc
     3121 gccctgctgc gcgaacgagc cgccgactcg gccgcatgcc ggccgttctc gctcgaggcc
     3181 ctcggggccg aaaccctaaa ccggctctgc gccgagcgcg acgagtcggt tccggccctg
     3241 ctgtctcggg cgttccagga catgcgcctc gggtacgccg ccgttggctc cgcggcgctc
     3301 acctgcgtgt cggtcctgct cgtcatcgtc atcttccact tcgcgccgct cagcgcgcgc
     3361 gccgactccc tggccggcat gatggacacc ctgggagccc ccgccatcgg cctggacagc
     3421 cgcgtggtgt cgccgccctc cggctggagc ctgatctctg aagacctggt gagcgaggag
     3481 gacgcggtct tcgcgctggc ggcggtggtc acgcgcaacg gccgcatcgc gagcctcgag
     3541 tacctctcgg accagtccac gaactcggag cgcgagcggg ccatcaggct gcttgacgag
     3601 ctgtcccgcg cgcggttcga gccggcgcgc ctgggcggca caccggtgac ggtgaaaacg
     3661 gtcctcgtgc tggcgcgcac caccgtgcgc ggaaagatgc ccccggcggc caaacagagc
     3721 cggcttcccc ggggaccgga cgccgtgcgc gggtagcgcc gccctgagcc gctagcggag
     3781 gcccgccccg cccactctcg ccgtcgcggt gttgttcatg ccgtcggtcg ctcggatgac
     3841 cagccggccc tccgccttcc cttcgatgcg gatcgcgtag cgctcgcggc gcgagtcggc
     3901 ggcgccgtca acggggtagg ccgcaatcca tcggcccgag tcgatcgagt actcgacgac
     3961 cgacagtggc gagtgcgagt ccgccacctc gaactccacg tcggaggacg acgctccctg
     4021 gaccgcgcgc aggatcgtga tggcgggcgc gctgttgtcc acgtcgaagg ccgcgctctc
     4081 gagttcgccg gtcagcgcct gctcggccgg gttgttcggc gcgtccgagg ccgtgacctt
     4141 cacgacgtac gtgccgtccg gcaccgacgt cgtgtcccac gtcacgagca gctccggcgc
     4201 gttggcgcgg agcgtcttcc actgggcgtc cccgacggcg cggaagaaca cggcgtactc
     4261 catccggtcg tcgttgtcgt cctcggcctt ccacgcgaac gcctgcaggc ccttctggta
     4321 catccgccga ccgagcgccg ggccggatgc cggcgtctgc tgggtcgagc cgagcggcgt
     4381 cgcgaaggcc ggcgcgcgcg cttcgccggg cacgtcgccc aggccggcga tctccggttc
     4441 tccggccggg tacggccgct ggaacacgat gccgggggga tgcacggtga tctcggtgac
     4501 gcgcggccgt gcgttgcgct gcaggtaggc aatgctgacc gacgccagcg cgggcgaggc
     4561 ggagccggcg aactggcctt tcagttcggc ccgccattgc aggtagcggg cggccgggct
     4621 cgtcacggca tctccttcgg cccgttgata cggcgcgctc cagccgctcc aggtgtcgtc
     4681 cggaacggcc gtattgccgg tgcgcgtggc gatgcgaatg gcgcccggtc cggctccgga
     4741 cgcacgccag gcaatcg
//
LOCUS       DAOSIF010000215         4747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_15988_length_4747_cov_3.427323, whole genome shotgun sequence.
ACCESSION   DAOSIF010000215 DAOSIF010000000
VERSION     DAOSIF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15988_length_4747_cov_3.427323"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLT35_08130"
     CDS             complement(<1..603)
                     /locus_tag="PLT35_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOG29233.1"
                     /translation="MDTDALSPLMSALRDAVLDGDARGAASATERALGQGVDPQRLVD
                     GALIPAMDEAGRRFENGEFFVPELLVSARALKASQAIVTPLLAQRGGGVARGRVAIGT
                     VKGDMHDIGKNLVVALLQGGGFDVVDLGTDVPPTRFVSAVTNDRADIVGLSALLTTTM
                     LQMKATLKAMETAGVRGSVKVNVGGAPVTQRFADEIGADGY"
     assembly_gap    1046..1055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1056..2097
                     /locus_tag="PLT35_08135"
     CDS             <1056..2097
                     /locus_tag="PLT35_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOG29234.1"
                     /translation="VRPGTGVRKEIASMPDQLSLFEAETPAPAQRRRAASPAPGSALE
                     QRYERYRVLAAALPPGLRMGTSSWSFPGWAGIVYARKRAVSMLARDGLYEYAQHPLLR
                     TVGIDRSYYAPIPDDDLRRYAEQLGDEFPCCAKAPASVTSPTIPGLGHHQPNPDYLSA
                     SRFTADVLEPFARCFARHTGPFVLQFPPPVSPQASDPAAFVERLDAFLAALPRQFQYA
                     VEIRDKWALTGAYQRVLARHGAGHVYNYWSAMPLPGRQARAVPPERQPFVVIRLLLRP
                     GTWYENQKQVFAPFDRLVQPDDEMRRDVLGLVRAAALASRPTYLLVNNKAEGSAPLTV
                     EALAEHYVAEGS"
     gene            complement(2173..3417)
                     /locus_tag="PLT35_08140"
     CDS             complement(2173..3417)
                     /locus_tag="PLT35_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29235.1"
                     /translation="MTSRLVPRTFAALALVVALAGALDLLAASAAQAQAQDNYKTLTR
                     LGGSNRFDQPIRSTAAVQKWAARKRTQSGLTTVMEKAGLSSLTSTVLDILTKASPDQL
                     KESDLQPGTTMVWMAFRRGGTRPDIVRNVRWGGKKPAPGFTFVIDDMNQTYTFFLPKP
                     CANLTLVTSEPSREKARLDAEKAAAERAAAEKAAAEKAAAEKARLEAERRERERAEAE
                     RLAQEKAAAERAAAERAAAEKAAAERAEAERIAREKDAKVDWFASPLFGKERRTRQFE
                     TAAGSALATGTSSLCSPLLAGKVGPEFKVGPNFKINPGIGGAINFEKSGNSSLFTEVE
                     FNGYTEGLKHYIGASVGLWDITHSDWMTPTLGLQAGTEIWSNAKEDRIYLVGEGRFFT
                     RAFGAGLSNNYQFWGGLRYVSR"
     gene            complement(3744..>4747)
                     /locus_tag="PLT35_08145"
     CDS             complement(3744..>4747)
                     /locus_tag="PLT35_08145"
                     /inference="COORDINATES: protein motif:HMM:NF018829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HOG29236.1"
                     /translation="FRTAPHIDREETHKRACDMLVHCLDAGIRPTLVWAPIPVLMPGE
                     RSSTEYEPAKRLWAQLPALNARDGVLDVSLLVGYVWADERRATAASVLTGTKPAVLHQ
                     TAAELAAQYWEARTEFAFGVPTGTMGEMIEKALALPTQPVVISDSGDNPTGGGTGDRA
                     DALAELLRMKVRNAVFAGIADRPATEACYAAGVGKTIALTVGGSLDPQASRPVPVTAR
                     VAFVERTDAPAERRAVVEVDGVTLALTARRRPFHEIRDFTALGLDPKAFKIVVVKAGY
                     LVPEIAAIANPNLMALTDGAVNQDIEHLTSVHRVPTYPFVRDLAYVPRTFVSARSKS"
BASE COUNT          630 a         1753 c         1645 g          709 t
ORIGIN      
        1 gtagccgtcc gccccgattt cgtcggcgaa gcgctgggtc acgggcgccc cgccgacgtt
       61 caccttcacc gacccgcgga cgcccgcggt ctccatggcc ttcagcgtcg ccttcatctg
      121 gagcatggtc gtcgtcagca gcgccgacag accgacgatg tcggcgcggt cgttcgtgac
      181 ggcggacacg aagcgcgtcg gaggcacgtc ggtcccgaga tcgacaacgt cgaagccgcc
      241 gccctgcagg agcgccacga cgaggttctt gccgatgtcg tgcatgtcgc ccttgacggt
      301 gccgatggcg acgcgcccgc gggcgacgcc gccgccgcgc tgcgccagca gcggcgtcac
      361 gatcgcctgc gacgctttca gcgcgcgcgc cgacaccagc agttccggca cgaagaactc
      421 gccgttctcg aagcggcgcc cggcctcgtc catcgccggg atgagcgcgc catccaccag
      481 ccgctgcgga tcgacgccct gccccagggc gcgctcggtg gccgaagccg ccccgcgcgc
      541 gtcgccgtcg agcaccgcgt cgcgcagggc cgacatcagg ggggagagtg cgtcagtgtc
      601 catggcttgc ctgcttcgcg cctgcctcca cgcgccgtgg gccagcgcac ccgggcccag
      661 ggaaatcggc ccgcgcctga gccttgcgac aggaacacgc gcgatccgcc cgctccgggc
      721 tgcagggccg ccgccagcgt gacgaagcct ccctgctctc cctccgccgc cattcgccgc
      781 ggcgcgcgcc cgctgcgggc gatgtccacc agcgcgagga agagggcgga gtcgaccgtg
      841 ccgcccgtgg ccatgccgac ctgcgtcaaa cggcgccgga tcccggccag gcgcagttcg
      901 ccccctagct cgcggcatgg cgcgaggcgc gcgcgtagcc taccatgcgg cccgacgcca
      961 gtgaaactcc ggcggccgga cgcgcgggcg gctccgcccg cccggccggg ttgcgggcgc
     1021 gggcgtccgt ctgccagaat cgcgtnnnnn nnnnncgtgc ggccgggaac gggcgtccgc
     1081 aaagagatcg cgtccatgcc ggaccagttg tcgctcttcg aggcggagac gccggctcca
     1141 gcgcaaagac gccgggccgc ctcgcccgcg ccgggttcgg ccctggagca gcgctacgag
     1201 cgctatcggg tcctggctgc cgcgctgccc ccggggctgc ggatgggaac cagttcctgg
     1261 agctttccgg gctgggccgg catcgtctat gcgaggaagc gcgccgtgtc gatgctcgcg
     1321 agggacggcc tgtacgagta cgcgcagcac ccgctgctgc gcacggtggg catcgaccgc
     1381 agctactacg cgccgatccc cgacgacgac ctgcggcgct acgcggagca gttgggcgac
     1441 gagttcccct gctgcgccaa ggcgccggct tcggtcacgt ccccgacgat tcccggcctc
     1501 ggccaccacc agccgaatcc cgactacctg tccgcgtctc ggttcaccgc ggacgtgctg
     1561 gagccgttcg cgcgctgctt cgcgcgccac accgggccgt tcgtccttca gttcccgccg
     1621 cccgtcagcc cgcaggcctc cgatcctgcg gcgttcgtcg agcgcctcga cgccttcctg
     1681 gccgcgctgc cgcgccagtt ccagtacgcg gtcgagattc gggacaagtg ggcgctcacc
     1741 ggggcgtatc agcgggtcct cgcccggcac ggcgccgggc acgtgtacaa ctactggtcc
     1801 gcgatgccgc tccctggccg gcaggcgcgg gcggtgccgc cggaacgcca gccgttcgtc
     1861 gtcattcggc tcctgctgcg gccggggacg tggtacgaga accagaagca ggtgttcgcg
     1921 ccattcgacc ggctcgtcca gccggacgac gagatgcgcc gcgacgtgct tggcctcgtc
     1981 cgggcggctg cgctcgcctc gcgcccgacg tacctcctcg tcaacaacaa ggccgagggc
     2041 tccgccccgc tcaccgtgga agcgctggcc gagcactacg tcgcggaggg gagctagtct
     2101 cccgctgtca gaaaagacgc gggcggcacc tcggtgccgc ccgcaaagcg tcacgcgcga
     2161 gagccggctg cgctagcggc tgacgtagcg caggccgccc cagaactggt agttgttcga
     2221 cagcccggcg ccgaaggcgc gggtgaagaa cctgccctcg ccgacgaggt agatccggtc
     2281 ctccttggcg ttgctccaga tctcggtgcc ggcctggagg cccagcgtcg gggtcatcca
     2341 gtcggagtgc gtgatgtccc acaggcccac gctggcgccg atgtagtgct tgagcccctc
     2401 ggtgtagccg ttgaactcga cttcggtgaa gagcgagctg ttgccgctct tctcgaagtt
     2461 gatggcgccg ccgattcccg ggttgatctt gaagttcggc ccgaccttga actccggccc
     2521 gaccttgccc gccaggagcg gggagcacag gctgctggtt ccggtggcca aggccgagcc
     2581 agccgccgtc tcgaactggc gcgtgcggcg ctccttgccg aagagcgggg acgcgaacca
     2641 gtcgaccttc gcgtccttct cgcgggcgat ccgctcggcc tcggcgcgct cggcggcggc
     2701 cttttcggcg gcggcgcgct cggcggcggc gcgctcggcg gccgccttct cctgggcgag
     2761 ccgctcggcc tcggcgcgtt cgcgctcccg ccgctcggct tccagccgcg ccttctcggc
     2821 ggcggccttc tcggcggcgg ccttctcggc ggcggctcgc tcggcagcgg ccttctcggc
     2881 atcgaggcgc gccttctcgc ggctcggctc gctcgtcacc agcgtcaagt tcgcgcacgg
     2941 cttcggcagg aagaacgtgt acgtctggtt catgtcatcg atgacgaagg tgaagccggg
     3001 cgcgggcttc ttgccgcccc acctgacgtt ccgcacgatg tcgggcctgg ttcctccgcg
     3061 gcggaacgcc atccacacca tggtcgtgcc cggctgcagg tccgactcct tcagctggtc
     3121 cggcgaggct ttcgtcagga tgtccaagac cgtcgaggtc agggacgaca gcccggcctt
     3181 ctccatgacg gtggtgaggc ccgactgcgt ccgcttcctc gccgcccact tctgcacggc
     3241 cgcggtgctc ctgatcggct gatcgaaccg gttgcttccg cccagccggg tcagggtctt
     3301 gtagttgtcc tgggcctgcg cctgggccgc cgaagcggcc agcaggtcga gggcgcccgc
     3361 gagcgccacc acgagcgcca gcgcggcgaa cgtgcgtgga acgagtcttg atgtcatgtt
     3421 ggccttcccc aatgggagcg agacaaaccc ttcgtgagcg acgcacccgg gagaacgcgc
     3481 gcgtcccggc aggtgatccg ggacatgata caacgaaacg acggctttcg acgagggcgc
     3541 ggcggggcca gacgcgctcg gctccggcct tccgccggcc gacgcgccgg caatcgcggc
     3601 ggccttcagc tcgcggtccc ggcacgaggc tcgacgttca ccccagaagc caggcgacgc
     3661 gcacggcccg acccgggccg tgcgctggcc gccgcccgcc cggcgccatc ccgatgccgc
     3721 gccgccgccc gtgcccgagg cggtcaggat ttcgatcgcg cggaaacgaa cgtgcgcggc
     3781 acgtacgcca agtcgcgcac gaaggggtag gtcggcaccc ggtgcacgct cgtcaagtgc
     3841 tcgatgtcct ggttgacggc gccgtccgtc agcgccatga gattcgggtt cgcgatggcc
     3901 gcgatttccg ggaccaggta gccggccttc acgacgacga tcttgaaggc cttcggatcg
     3961 aggcccaacg ccgtgaagtc ccggatctca tggaagggcc ggcggcgcgc cgtgagcgcc
     4021 agcgtcacgc cgtccacctc gaccacggcg cgccgctccg cgggagcgtc cgtccgctcg
     4081 acgaacgcca ctcgggccgt gacgggcacg gggcggctgg cctgcgggtc gaggctgccg
     4141 cccacggtga gcgcaatcgt cttcccgacg cccgccgcgt agcacgcctc cgtcgccggc
     4201 cgatcggcga tgccagcgaa gacagcgttc cggaccttca tccgcagcag ctcggccagg
     4261 gcgtcggcgc gatcgcccgt tccgccgccc gtcgggttgt cgcccgagtc ggagatcaca
     4321 accggctgcg tcggcagcgc gagggccttc tcgatcattt cccccatcgt gcccgtcggc
     4381 acgccgaacg cgaactcggt gcgggcctcc cagtactgcg cggccagctc cgcagccgtc
     4441 tgatgcagca ccgcgggctt cgtgccggtg agcaccgaag cggccgtagc ccgccgctcg
     4501 tccgcccaga cgtacccgac gagcagggag acgtcgagca cgccgtctcg cgcgttcaag
     4561 gccggcagct gggcccagag ccgcttggcg ggctcgtatt ccgtgctgct gcgctcgccc
     4621 ggcatgagca ccggaatcgg cgcccacacg agcgtcggcc ggatcccggc gtcgaggcag
     4681 tgcacgagca tgtcgcaggc gcgcttgtgg gtctcttcgc ggtcgatgtg aggcgcggtg
     4741 cggaagg
//
LOCUS       DAOSIF010000216         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16020_length_4738_cov_4.177237, whole genome shotgun sequence.
ACCESSION   DAOSIF010000216 DAOSIF010000000
VERSION     DAOSIF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16020_length_4738_cov_4.177237"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLT35_08150"
     CDS             <1..204
                     /locus_tag="PLT35_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29237.1"
                     /translation="DLDQPGLAAFKDHLGAERAALAYCAWPVRRARPPQPGALSRLAR
                     SVVPLLPDAVLDWSGRMLYRHLG"
     gene            274..1455
                     /locus_tag="PLT35_08155"
     CDS             274..1455
                     /locus_tag="PLT35_08155"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG29238.1"
                     /translation="MSDNPVPFLDLVAPHRELENDLVEAFRQAVRSAAFVGGAEVEGF
                     EKEFAEFCGSKFCVGVANGTDAVRFALMASGVGPGDAVVTVAHTFIATVEAISQAGAE
                     TEFVDIDERTYCMSPAALDEYLKGCAKDPATGRPLGRRTGKPIKAVLPVHLYGQTADM
                     DAILAVADRYGLIVVEDACQAHGAEYKSADGKWRRAGTFGKAAAFSFYPGKNLGSCGE
                     GGAVTTDDEQAAKVIRMLREHGQAQKYYHDLEGYNGRLHAIQAAFLRVKLRRLPEWNA
                     GRYAAARRYGEMLAGVPGVIVPHEPENTRPVYHLYVIRTKDRDALGEHLKARKVFTGL
                     HYPVPLHLQKCYAGWGYAKGSLPVTEAVASSIVSLPMFPTLTAEQQRRTVDAVADFVA
                     R"
     gene            1474..2535
                     /locus_tag="PLT35_08160"
     CDS             1474..2535
                     /locus_tag="PLT35_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HOG29239.1"
                     /translation="MSSRRIWIDLDNTPHIPFFLPIIRELEREGHSVVLTARDAYGVC
                     DLAAHHGLAVTTVGRHYGKHRALKVAGTLWRALQLAPVILRERPDISMSHGSRSLVIL
                     SGLLRIPSLLLFDYEHAERIPLLRPALGMAPASIDDPSLAKGFRYGVRHYSGLKEDVY
                     AAGFRPDPSILDELGVGPSEILVTIRPPATDAHYHNPEAEGLYVEVVEMLGAMPGVRM
                     VVLPRTAGAQRDFLMTTWPAWCAEKKIVVPERALDGLNLVWHSDLVVSGGGTMNREAA
                     ALGVPVYSIFRGKLGAVDRQLAREGRLVLLATRDDVRSKLRVARRAKGASFAAGDRPA
                     LRQILAAARELMELSNGRP"
     gene            2522..3685
                     /gene="wecB"
                     /locus_tag="PLT35_08165"
     CDS             2522..3685
                     /gene="wecB"
                     /locus_tag="PLT35_08165"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOG29240.1"
                     /translation="MGARSAPVQVHLVCGARPNFMKVAPLYHALAAEPWADPVIVHTG
                     QHYDANMSGAFFADFGLPEPHVSLGVGSGTHAEQTAKVMVAYERVLQERRPDLVVVVG
                     DVNSTAAATLAAAKLGVKVAHLEAGLRSRDRRMPEELNRLVTDVLADLLWAPSQDGVE
                     NLLAEGIPAGRIQLVGNIMIDTLEMCRERIERQDAFSAFGLRPGGFAVATIHRPSNVD
                     DPAVLGPLCAILESVSELVPLVFPVHPRTGRRIAAEGLLAGARRAGRLFLPEPMPYIA
                     FMNLVFNARLVITDSGGIQEETTYLGIPCLTMRENTERPITVTQGTNRLCAPDRLLAS
                     VREILRRRPPSAPRIPYWDGRTAGRVVEAIRGQFAWRAGAATAEAAPLAVPRS"
     gene            3682..>4738
                     /locus_tag="PLT35_08170"
     CDS             3682..>4738
                     /locus_tag="PLT35_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29241.1"
                     /translation="MISNARLEEIVTRLEAYCRSRDWAGYDPYDALNSGRFARTPLAR
                     SRLARLALTQLLKRSPVNFRPLLGIVPHQNPKALGLFLSFYGRRLVAGDAGAAAPARA
                     VAARLLELRSPGAGCWCWGYSFPWQMRTRLVPRFAPNLVCTTFAAHGLLDAYDAGLGD
                     EWLAPAVGAGEYLARELFWEDGQRAAFAYPLPDIRVPIHNANFLGAALLCRLARTTGD
                     DGVVAPALRAARWSAARQREDGSWPYGEGPTQQWMDNFHTGFNLCALESIGRDLGTRE
                     FELALRKGFQFYLDHFFAPDGVPRYFHDRTYPIDIHSVAQSLITLTSLRALDERAAAV
                     ARSVLAWALANMWDERGF"
BASE COUNT          657 a         1726 c         1626 g          729 t
ORIGIN      
        1 gatctcgacc agcccggtct cgccgccttc aaggaccatc tgggcgccga gcgggcggcc
       61 ctggcctact gcgcctggcc ggtccgccgg gcgcgcccgc cgcagccggg cgcgctgtcg
      121 cgcctcgcgc gcagcgtcgt tcccctgctg ccggatgcgg tcctcgactg gtccggccgg
      181 atgctgtacc ggcacctggg ttagcagcct gagcgccttt tcagatggag ccccggggcg
      241 ttcgccccgg gattgacccc cgggagcacc gacatgtccg acaaccccgt tccttttctc
      301 gacctcgtcg ccccgcatcg cgaactcgag aacgacctcg tcgaggcctt ccgccaggcg
      361 gtgcgctcgg ccgccttcgt cggcggcgcc gaggtcgagg gcttcgagaa ggagttcgcc
      421 gagttctgcg ggtcgaagtt ctgcgtgggc gtggccaacg ggaccgacgc cgtgcgcttc
      481 gccctgatgg cctccggcgt gggcccgggc gatgccgtcg tcaccgtggc gcacacgttc
      541 atcgccaccg tcgaggccat cagccaggcc ggggccgaga cggagttcgt ggacatcgac
      601 gagcgcacct actgcatgag ccccgcggcc ctcgacgagt acctgaaggg ctgcgcgaaa
      661 gatcccgcca ccggccggcc cctgggcagg cggaccggca agcccatcaa ggccgtcctt
      721 cccgttcacc tctacggcca gaccgcggac atggacgcca tcctggccgt cgccgaccgc
      781 tacggcctga tcgtcgtcga ggacgcctgc caggcgcacg gggccgagta caagtcggcc
      841 gacggcaagt ggcgcagggc cggcaccttc ggcaaggccg ctgctttcag tttctatccc
      901 ggcaagaacc tcggctcgtg cggcgagggc ggggccgtga cgaccgacga cgagcaggcg
      961 gcgaaggtca tcaggatgct gcgcgagcac gggcaggcgc agaagtacta ccacgacctc
     1021 gagggctaca acggccggct ccacgccatc caggcggcgt tcctgcgggt gaaactgcgg
     1081 agactccccg aatggaacgc cggccggtac gccgccgcgc ggcggtacgg ggagatgctc
     1141 gccggggtgc ccggggtgat cgtgccgcac gagccggaga acacgcgccc cgtctaccac
     1201 ctctacgtca tccggacgaa ggaccgcgac gcccttggcg aacacctgaa ggcgaggaag
     1261 gtgttcaccg ggttgcacta ccccgtgccg ctgcaccttc agaagtgcta tgccggctgg
     1321 ggctatgcga agggcagcct gcccgtcacc gaggccgtcg ccagctcgat cgtctcgctg
     1381 cccatgttcc cgacgctgac cgcggagcag cagcggcgca ccgtcgacgc cgtcgccgac
     1441 ttcgtcgcca ggtgagagac agacgcgcct ctgatgtcat cccgtcgcat ctggatcgac
     1501 ctcgacaaca ccccgcacat cccgttcttc ctccccatca tccgggagct cgagcgcgag
     1561 gggcactccg tcgtgctgac cgcgcgcgac gcgtacggcg tctgcgatct cgccgcccac
     1621 cacggcctcg ccgtaacgac ggttggccgg cactacggca aacacagggc gctgaaggtc
     1681 gcgggcacgc tctggcgcgc cctgcagctc gcgcccgtga tcctgcgcga gaggcccgac
     1741 atctccatgt cgcacggctc gcgctcgctg gtgatcctct caggcctcct gcgcatcccg
     1801 agcctgctcc tcttcgacta cgagcacgcc gagcgcatcc ccctgctgag gcccgccctg
     1861 ggcatggcgc ccgcctcgat cgatgacccg tcactggcga aggggtttcg ctacggggtg
     1921 cgtcactact ccggtttgaa ggaggacgtc tacgcggcgg gcttccggcc ggacccctcg
     1981 atcctggacg aactcggcgt gggcccctcg gagatcctcg tcaccatccg cccgcccgcg
     2041 accgacgcgc actatcacaa tcccgaggcc gaggggctgt acgtcgaggt cgtcgagatg
     2101 ctgggcgcga tgcccggcgt ccgcatggtc gtgctgccca ggacggccgg cgcccagcgc
     2161 gacttcctga tgacgacgtg gcccgcgtgg tgcgcggaga agaagatcgt cgtccccgag
     2221 cgggccctcg acggcctcaa cctcgtgtgg cactccgacc tcgtcgtgag cggcggcggc
     2281 accatgaacc gcgaagccgc ggccctcggc gtgcccgtgt acagcatctt ccgcggcaag
     2341 ctgggcgccg tggacaggca gctggccagg gaaggccgcc tcgtgctgct cgcgacgcga
     2401 gacgacgtgc ggtcgaagct gcgggtggcc aggcgcgcga agggcgcgtc gttcgccgcg
     2461 ggcgaccggc ccgccctccg gcagatcctg gccgccgcgc gcgagctgat ggagttgtcg
     2521 aatgggcgcc cgtagcgctc ccgtgcaggt tcacctcgtg tgcggggccc gccccaactt
     2581 catgaaggtg gcgccgctct atcacgccct cgccgccgag ccctgggccg acccggtcat
     2641 cgtccacacc ggccagcact acgacgccaa catgtccggg gcgttcttcg ccgacttcgg
     2701 cctgcccgag ccgcacgtgt cgctcggcgt cggcagcggc acgcacgccg agcagaccgc
     2761 caaggtcatg gtggcctacg agcgcgtcct gcaggagcgg aggcccgacc tggttgtcgt
     2821 cgtgggcgac gtcaattcca ccgcggccgc cacgctggcg gcggcgaagc tcggcgtgaa
     2881 ggtggcgcac ctcgaggccg gcctgcggtc gcgcgaccgg cgcatgcccg aggaactcaa
     2941 tcggctcgtc accgacgtgc tggcggatct gctctgggcg ccgtcgcagg acggcgtcga
     3001 gaacctcctg gccgagggga ttcccgccgg gcggatccag ctcgtcggca acatcatgat
     3061 cgacacgctc gagatgtgca gggagcgcat cgagcggcag gacgcgttca gcgcgttcgg
     3121 gcttcggccg ggcggctttg ccgtggcgac catccaccgg ccgtcgaacg tggacgaccc
     3181 ggccgtcctc ggcccgcttt gcgccattct cgagagcgtg tccgagctcg tgccgctcgt
     3241 gtttcccgtg cacccgcgga ccggccggcg cattgccgcc gagggcctgc tggctggggc
     3301 ccggcgggcc gggcgcctct tccttcccga gcccatgccg tacatcgcgt tcatgaacct
     3361 cgtgttcaac gcgcggctcg tcatcaccga ctcgggcggg atccaggagg agaccaccta
     3421 tctcggcatc ccgtgcctga ccatgaggga gaacaccgag cggccgatta ccgtgacgca
     3481 gggcaccaac cggctgtgcg cgccggatcg gcttcttgcg agcgtgcggg agatcctgcg
     3541 gcgccggccg ccgtccgcgc cgcgcattcc gtactgggac ggcaggaccg ccggcagggt
     3601 ggtcgaggcc attcgcggcc agttcgcctg gcgcgccggc gccgccacgg ccgaggcggc
     3661 gccgctcgcg gtgccgcgct cttgatctcg aacgctcgcc tcgaggagat cgtcaccagg
     3721 ctcgaggcgt actgccggag ccgcgactgg gccggctacg atccgtacga cgcgctgaac
     3781 agcgggcggt tcgcgcggac gcctcttgcg aggagccgcc tggcgcgcct cgcgctcacg
     3841 cagctcctga agcgcagccc cgtcaatttc cgcccgttgc tcggcatcgt cccgcatcag
     3901 aaccccaagg ccctcgggct gttcctgtcc ttctacgggc ggcgcctcgt ggctggggat
     3961 gccggggccg ccgcgccggc cagggccgtt gccgccaggc tgctcgagct tcgatcgccc
     4021 ggggccggct gctggtgctg gggctacagc ttcccgtggc agatgcggac caggctcgtg
     4081 cccaggttcg cgcccaacct cgtctgcacc acgttcgcgg cgcacggcct gctcgatgcg
     4141 tacgacgccg ggcttgggga cgagtggctc gcgcccgccg tcggggccgg cgagtacctg
     4201 gcgcgcgagc ttttctggga ggatgggcag cgggccgcgt tcgcgtatcc cctgccggac
     4261 attcgggtcc ccatccacaa cgccaatttc ctcggcgccg cgctcctttg ccggcttgcc
     4321 cggacgacgg gcgacgacgg agttgtagcg cccgcgctgc gtgccgcgcg gtggtcggcc
     4381 gcgaggcagc gcgaggacgg ctcgtggccg tacggagagg gcccgaccca gcagtggatg
     4441 gacaacttcc acaccggctt caacctctgc gcgctggagt ccatcggccg cgatctgggg
     4501 acccgcgagt tcgaactcgc gctccggaag gggttccagt tctacctgga ccacttcttc
     4561 gcgccggacg gcgtgcccag gtacttccac gatcggacgt atcccatcga cattcacagc
     4621 gtcgcgcaga gcctgatcac gctgacttct ctgcgtgcgc tggatgagcg agcggccgcg
     4681 gttgcccggt ccgttctcgc ctgggcgctc gccaacatgt gggacgagcg cgggttct
//
LOCUS       DAOSIF010000217         4733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16039_length_4733_cov_3.923044, whole genome shotgun sequence.
ACCESSION   DAOSIF010000217 DAOSIF010000000
VERSION     DAOSIF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16039_length_4733_cov_3.923044"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLT35_08175"
     CDS             complement(<1..425)
                     /locus_tag="PLT35_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG29242.1"
                     /translation="MTRRDFVRMTACGAAGGAALWLPRLDRAAAEQAASPAGRFDAVI
                     AGGGLGGVAAALAAARAGLRVAMTEETDWIGGQLTQQGVPPDEHQWIETHGSTRTYRE
                     LREGIRDYYRRYYPLTDAARTRVALNPGDGSVSRLCHEPR"
     gene            complement(878..1960)
                     /gene="buk"
                     /locus_tag="PLT35_08180"
     CDS             complement(878..1960)
                     /gene="buk"
                     /locus_tag="PLT35_08180"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOG29243.1"
                     /translation="MPDGRRFRILVINPGSTSTKIAAYLDDCAEMSETLRHSGEEMAP
                     YRGRPVLDQIAWRAGVIESALGRAGISEDSFDAVAGRGGLLPPLASGTYLVNDRMLEE
                     LRLARRGEHASNLGACLAQAIAARAGVAAYVVDPVSVDEWEPVARVSGCALIDRECLW
                     HALNSKAVAKRYARERGVPYESLRLVVAHIGGGASISVHRGGLGVDVINPREEGPFSM
                     DRAGGVPVLKLASLCFSGQHRLADIERMLFGEGGVYSHLGTKDLIEVERRIAQGDERA
                     RLVFEAMAYQIAKAIGAMATVLHGRIDAVLLTGGMAHSSRLVEAIRGSVAWIAPLAVY
                     PGEDEMQALAEGVLRVLRGEEQARQF"
     gene            complement(1947..2852)
                     /locus_tag="PLT35_08185"
     CDS             complement(1947..2852)
                     /locus_tag="PLT35_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOG29244.1"
                     /translation="MLTSHAEIRTAARQRGPLRVAVVDADDDVALTAAALAAEQGIAT
                     AVLVGDEARIRERAGRLGLDAALATAEYAAADAPAAAAARLAADGGADMLLKGRLRTD
                     ELMRAVLDKSLGLRTGRLLSDVLLYEDVVSGTRRLVGVTDGGLNVAPDLAQKKQIVLN
                     AIGVLRAVGLARPKIAILSATEAVSAAVPSTVDARALTEMGEAGEFGDADVFGPLALD
                     NALLESAARGKGITHPVAGHADCLVVANIETGNALAKAAKYLAGSSCAHVVVGARLPI
                     LIPSRVESAEDKLNAIALGVLVAGR"
     gene            complement(2864..>4733)
                     /locus_tag="PLT35_08190"
     CDS             complement(2864..>4733)
                     /locus_tag="PLT35_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOG29245.1"
                     /translation="LVVVVPNYRVGVLGFLAHPGLSAESPVKASGNYGILDQIAALQW
                     VRDNIAAFGGDADRVTIAGQSAGGMSVHALIASPLAAGLFHRAIVQSGGSSVGGGGIA
                     LGARTLADAEAAGRQWAEARGAASVAELRAMSWQQLVEPRPAPAAGGGAPPAAMRFSP
                     IIDGYVTPAPVREVVVSGRQNDVPVLTGANTGELGGVSGPGAPVTLESFTKRAGQQYG
                     PLAPEFLALYPAKTDEEAAAAQARSARDLALVSMHLWARERAGTAKTPAYTYLWDHAL
                     PGPDAARYGAFHSSEVPYVLNTLYAADRPFTDQDRKIADMMSSYWANFAATGDPNGEG
                     LPRWNPAGQAPEVMQVGDRTGVVPIAGSPAAVAFFEKFLTRPLFAASAENYRIDVLGR
                     GLWRIQAITGTLSTAYLIAGSREALVVDACAGRDGFRDIVQQLVGNMPVKLALTHGHG
                     DHTGAVTHFSEVYVHQADAGMLPRTVTAARRPIDETTVFDLGGKRIEVVAIPGHTPGS
                     VAFVDRAGRYMMTGDGIGSTMVWMQISNLPLTAYLASVKKLEALKGAVDTLYVGHHEQ
                     ERRTLGPWYITDMRIVTEKVISGTIDTSPYAMAGGRGGLQASYGSATLVFHPDRIR"
BASE COUNT          613 a         1759 c         1700 g          661 t
ORIGIN      
        1 cggggctcgt ggcagagccg ggagacggac ccgtcgccgg gattgagggc cacgcgcgtc
       61 ctcgccgcgt cggtcagcgg gtagtagcgg cggtagtaat cgcggatgcc ttctcgcagc
      121 tcccggtagg tgcgggtcga gccgtgcgtc tcgatccact ggtgctcgtc gggcgggacg
      181 ccctgctgcg tgagctggcc gccgatccag tcggtctcct ccgtcatcgc gacgcggagc
      241 ccggcgcggg ccgccgccag cgcggcggcc acgccgccaa ggccgccgcc ggcgatgacg
      301 gcgtcgaacc ggcctgcggg agaggccgcc tgctccgccg ccgcccgatc gaggcgcggc
      361 agccacagcg cggcgccccc ggctgctccg cacgccgtca tccgcacgaa gtcgcgtctc
      421 gtcatggcct ggcaccccgc ctcggcgtct cccgggactc ttcgatcggc cgcgcgcccg
      481 ggccgccgcg cggggccatg atcgcacagt cggcccggcg gcacgacctc gttgccgtgc
      541 ggcgatccct gacgggcggg aaggccgcct tcgctccagg ccatcgtgtt gagtgcctgg
      601 gctgggccgc atcgcacgat ggaaggagaa ggcatggccg ccgatcgccg gcaggcccgc
      661 cgccgctgtt gttgacaagt gaactgaccg ctttcgtcag cgtgtcgcgg tcagagccgg
      721 tgggcgctgt gggccggtcg cgcttctggc gaccggtcca accggcttgt gcgcggccgg
      781 gctcgcccgg cgcagcatgc ccgggtcgtc cctttcattc caagctcccc tcggcgacgg
      841 gtccgctgga agtccgcgcc gcgggccggg gcgcggctca gaactgccgc gcctgctcct
      901 cgccgcgcag gacccggagc accccttcgg cgagcgcctg catctcgtcc tcgcccgggt
      961 agaccgcgag cggcgcgatc caggcgaccg agccgcggat cgcctccacg aggcgggacg
     1021 aatgcgccat gccgcccgtg aggagcacgg cgtcgatccg gccgtgcagg acggtggcca
     1081 tggcgccaat cgccttggcg atctggtagg ccatcgcctc gaagacgagc ctggcccgct
     1141 cgtcgccctg ggcgatgcgc cgttcgacct cgatgaggtc cttcgtgccg aggtgcgagt
     1201 acacgccgcc ctcgccgaag agcatccgct cgatgtcggc gagccggtgc tgcccggaga
     1261 agcagaggct cgccagcttg agcaccggga cgccgccggc ccgatccatc gagaacggcc
     1321 cttcctcccg cgggttgatg acgtccacgc ccagcccgcc gcggtggacc gagatcgacg
     1381 cgccgccgcc gatgtgcgcg accacgagcc tcagcgactc gtacggcacg ccgcgctcgc
     1441 gggcgtaccg cttcgccacc gccttcgagt tcagcgcgtg ccagaggcac tcgcggtcga
     1501 tgagcgcgca gccggacacg cgggcgaccg gctcccactc gtccaccgac acgggatcca
     1561 cgacgtaggc cgccacgccg gcgcgcgccg cgatggcctg cgcgaggcag gcgccgaggt
     1621 tcgacgcgtg ctcgccccgc cgcgccagcc gcagctcctc cagcatgcgg tcgttgacga
     1681 ggtaggtgcc gctcgccagc gggggcagca ggccgccccg gccggcgacg gcgtcgaacg
     1741 agtcttcgct gatgcccgcg cgcccgagcg ccgactcgat cacgcccgcg cgccacgcga
     1801 tctgatccag gaccgggcgg ccgcgatacg gcgccatctc ctcgccggag tggcggaggg
     1861 tctcggacat ctcggcgcaa tcgtccaggt acgccgcgat cttcgtggac gtcgagccgg
     1921 ggttgatgac gaggatgcgg aagcgcctac cgtccggcaa cgagcacccc cagggcgatg
     1981 gcgttcagtt tgtcttccgc gctctcgacg cgcgacggga tcaggatcgg cagccgggcg
     2041 ccgaccacga cgtgcgcgca cgacgacccg gccaggtact tggccgcctt ggcgagggcg
     2101 ttgcccgtct cgatgttggc gacgaccagg cagtcggcat ggccggcgac cgggtgcgtg
     2161 atgcccttgc cgcgcgcggc cgactcgagc agcgcgttgt cgagggcgag gggcccgaag
     2221 acgtcggcgt cgccgaactc gccggcctcg cccatctcgg tgagggcgcg ggcgtcgacg
     2281 gtggaaggca ccgcggcgga gacggcctcg gtcgcgctca ggatggcgat cttggggcgc
     2341 gccaggccga cggcgcgcag cacgccgatc gcgttcagca cgatctgctt cttctgggcg
     2401 agatcgggcg cgacgttcag gcctccgtcg gtcacgccca ccaggcggcg cgtcccggac
     2461 accacgtcct cgtagagcag cacgtcgctg aggagcctgc cggtgcgcag gccgaggctc
     2521 ttgtcgagga cggcgcgcat cagctcgtcc gtccggaggc ggcccttgag cagcatgtcc
     2581 gcgcccccgt cggccgccag ccgggccgcc gccgccgccg gcgcgtcggc cgccgcgtac
     2641 tcggcggtgg cgagggcggc atcgagcccg agccgcccgg cgcgctcgcg gatccgcgcc
     2701 tcgtcgccga cgagcaccgc ggtggcgatg ccctgctcgg cggccagcgc agccgccgtc
     2761 agcgccacgt cgtcgtccgc gtccaccacg gcgacgcgca gcgggccgcg ctgcctggcg
     2821 gcggtccgga tctctgcgtg agacgtcagc atgaagacct ccgctacctg atgcggtccg
     2881 ggtggaagac gagcgtcgcc gacccgtacg acgcctgcag gcctccccgg cctccggcca
     2941 tcgcatacgg gctcgtgtcg atggtgccgc tgatcacctt ctcggtgacg atgcgcatgt
     3001 cggtgatgta ccagggcccc agcgtccgcc gttcctgctc gtggtggccc acgtacagcg
     3061 tgtccacggc gcccttcagc gcttcgagct tcttcaccga cgccaggtac gccgtgagcg
     3121 gcaggttcga gatctgcatc cacaccatcg tcgagccgat gccgtcgccg gtcatcatgt
     3181 aacggccggc ccggtcgacg aacgcgaccg accccggcgt gtggcccggg atcgcgacga
     3241 cctcgatccg cttgccgcca aggtcgaaga ccgtcgtctc gtcgatcggg cgccgcgccg
     3301 cagtgactgt cctgggcagc atccccgcgt ccgcctggtg gacgtagacc tcggaaaagt
     3361 gcgtcaccgc gccggtgtga tcgccgtggc cgtgggtcag cgccagcttc acgggcatgt
     3421 tgccgacaag ctgctgcacg atgtcccgga acccgtcccg gcccgcgcag gcgtcgacga
     3481 cgagcgcctc ccggctgccc gcgatgaggt acgccgtcga cagcgtgccc gtgatggcct
     3541 ggattcgcca gagcccccgg ccgagcacgt cgatcctgta gttctcggcc gacgccgcga
     3601 agagcggccg cgtgaggaac ttctcgaaga aggcgaccgc tgcggggctt cctgcgatcg
     3661 gcactacgcc tgtgcggtcc ccgacctgca tgacctcggg ggcctggccg gccgggttcc
     3721 accggggcag tccctcgccg ttgggatcgc cggttgccgc gaagttcgcc cagtaggacg
     3781 acatcatgtc ggcgatcttc cggtcctggt ccgtgaaggg gcggtccgcc gcgtagagcg
     3841 tgttcagcac gtacgggacc tcggagctgt ggaacgcgcc gtagcgggct gcgtcgggcc
     3901 cgggcagggc gtggtcccac aagtacgtgt aggccggtgt ctttgccgtg ccggcgcgct
     3961 cgcgcgccca gaggtgcatc gacacgagcg ccagatcgcg ggcgcttcgc gcctgggccg
     4021 ccgccgcttc ctcgtcggtc ttcgccgggt agagcgcgag gaactccggg gccagcgggc
     4081 cgtactgctg cccggcgcgc ttcgtgaagg actcgagcgt gaccggggcc cccgggcccg
     4141 acacaccgcc gagctcgccg gtattggcgc cggtcagcac cgggacgtcg ttctggcgcc
     4201 ccgacaccac gacttcgcgc acgggcgccg gcgtgacgta tccatcgatg atgggcgaga
     4261 accgcatggc ggccggcggc gcgccgcctc cggcggccgg cgcgggccgc ggctcgacga
     4321 gctgctgcca gctcatcgcg cgcagctcgg cgaccgatgc cgcgcccctg gcctcggccc
     4381 actgcctgcc cgcggcctcg gcgtccgcca gcgtgcgcgc gcccagggcg atgccgcccc
     4441 cgccgacgct cgagccgccg ctctgcacga tggcccggtg gaacaagccc gccgcaagcg
     4501 gcgaggcgat cagggcgtgc accgacatgc cgccggcgga ctggccggcg atggtcacgc
     4561 ggtcagcgtc gcccccgaag gcggcgatgt tgtcgcgcac ccactgcagc gccgcgatct
     4621 ggtcgaggat gccgtagttc cccgacgcct tcaccggtga ctccgctgac agcccggggt
     4681 gcgccaggaa tcccagcacg ccgacgcggt agttggggac gaccaccacg agc
//
LOCUS       DAOSIF010000218         4729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16054_length_4729_cov_4.004707, whole genome shotgun sequence.
ACCESSION   DAOSIF010000218 DAOSIF010000000
VERSION     DAOSIF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16054_length_4729_cov_4.004707"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="PLT35_08195"
     CDS             <1..1856
                     /locus_tag="PLT35_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29246.1"
                     /translation="LCVADSADRPQRVALFLFAQRAFAGLSPVRLPDALVALRAFPRY
                     RVLLMTLERIGVIDPGIYAAAIRQAQRIDGIGDREKAELALRQFQGALALVERVRFSR
                     ALTAEGAAGLVSALAAVPLSQDNEYLGGIGAWLDDHFLPALASLPGAHGAPAAPDRPL
                     ESAVLEAMAGLRTRVEEVRVEWEGLLYRVDVGAAEFDRLSRLRRKQGGASLDAALALS
                     RASRGLAGPLPGLSVIPSRIEALAAAADQLASVRPAASDAAVRLRQAVAGATRDLQKI
                     TKAKDLEKAARISVPLRRASDGWLADVLLSLAYTPHLGDPDGPALLAGDPALRHRFSA
                     ENPVAGQRAAARWRIPVETLGEAGGWRATGSVLGLDVGLAALSLRRLTTDSLPPPPSV
                     NEIDRSSIAATLVLTNAFDVTDAGRDALAGAIARGRARVEALANDSSGVADVAQASGI
                     GEWRRQILPWALRHEPARAAEYFSLSEFARLGSAQPARQPDLDAWGTSALAREGCLCL
                     RYAEPGGWETLAGRFGTALVAEQMPDLSLRIAESLAGLGLPARLLPAVLAFVAQDVLD
                     TCAPAHLDDWTALAAAVRRLPDSRIADDIAALTAGGPLVPADREWRDDARR"
     gene            1840..3021
                     /locus_tag="PLT35_08200"
     CDS             1840..3021
                     /locus_tag="PLT35_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG29247.1"
                     /translation="MMRGADVLAIGPRQPRRAWLLAAALAAAGLAAQAPEPSVVFLSP
                     HSGDYTSGPTPIRVQVDPPGTAVSSLSLFADGRLVCTLDHPPFECAFDAGPNVVEHAL
                     RAVATLPGGRRIAQSVRTQGVAYTESVDVDVVQVTVTVTDGAGRFVRGLTRQDFRLFE
                     DNVRQDVTTFIAENVPTELIVAVDVSGSMTDAMPNVKESVKRFLSAIRPTDRVTVLGF
                     NDSVFTLARPTVDLAARLRAIDRLAPWGGTALYDVIIQAFDLLGRQTGRRALVVFTDG
                     EDLNSRAPIETAERRLEASDAVMYPIGQGRAQKLQSLRAVLERLSRKSGGRAFFGDDV
                     EKLDGVFGDILEELSNQYLLGYVPRDLRRDGRWRILKVEVPGKELRVRARQGYRAEPK
                     K"
     gene            3055..>4729
                     /locus_tag="PLT35_08205"
     CDS             3055..>4729
                     /locus_tag="PLT35_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG29248.1"
                     /translation="MANRAGVACAAVALAALLQGPLVPVTALAQQPPSFKSGVDVIAV
                     DVSIVDKEGQPVGGLDADRFEVAVDGKPRRVVSATFVDYASAAAGAGSAPLTGRPLQL
                     GASFASNEREEEPSAIPGRVVVLAVDQLSFAPGAARAAIESAQRFLDRLQPADRVGVV
                     AYPAPGPVVAPTVEHGVVRAALERVVGLGETGQPVAPYLSMSEAMALDRNDPQVRQQV
                     IERECAGLKQPAIRGAFSEIESCVRRIDGLVPQMLMQLQTQGARSLNGLQSVVNDLDR
                     ISGVKTLIVISAGMLPSSSARLDLRRDIQAVAAAAASANVRIYVLHVQREFLQAVSAE
                     MARPSPTVFDNEIVLASGLQMLAGLSAGSIFTVASGADRAFDRVARESSAAYILGIEP
                     EAGDRDGKSHRIQVRVRNLPNALVRSRSSFVVPAPSAAPPTPEAAVTAALKPGRLAKD
                     LPVQFTAHAMRDPRTGEIRLLMSANIGRGVAGPAEVRVGYAVSDQTGRVLGSAVDKLH
                     LDQRGAGPSASWSYLNAIGLRPGAYTLRVAAADEGGRTGSAEYRIDAKLT"
BASE COUNT          608 a         1696 c         1739 g          686 t
ORIGIN      
        1 gcctgtgcgt cgcggactct gccgaccggc cgcagcgggt ggcgcttttc ctgttcgccc
       61 agcgcgcgtt cgcggggctg tctccggtcc ggctgcctga cgcgctcgtg gcgctccggg
      121 cgttcccgag ataccgtgtc ctcctgatga cgctggagcg gatcggcgtc atcgatcccg
      181 ggatctacgc cgccgcgatc cgccaggccc agcgcatcga cggcatcggc gaccgcgaga
      241 aggccgaact ggcgctccgc cagttccagg gcgcgctcgc gcttgtcgag cgggtccgct
      301 tcagccgggc cctgacggcc gagggcgcag ccgggctcgt ctccgccctg gccgccgtgc
      361 ctctctcgca agacaacgag tacctgggcg gcatcggcgc gtggctcgac gaccacttcc
      421 ttccagcgct ggcgtcgctc ccgggcgcgc acggcgcgcc cgccgccccg gaccgcccgc
      481 tcgaatcggc cgtgctcgag gcgatggcgg ggctgcgcac gcgggtcgag gaggtccgcg
      541 tcgaatggga agggctgctt taccgggtgg atgtgggagc ggccgagttc gaccggctct
      601 cgcgccttcg ccggaaacag ggcggcgcca gcctcgacgc ggcacttgcc ctgtcgagag
      661 cgtcccgcgg gctggctggc ccgctgcccg ggctctcggt cattccatct cggattgagg
      721 ccctcgcggc ggccgcggac cagctcgcgt cggttcgacc ggcagcttcg gacgcggcgg
      781 tccgactgcg tcaggcggtc gccggggcga cgcgcgacct gcagaagatc acgaaggcca
      841 aggacctcga gaaagccgcc aggatctccg tcccgctccg gcgcgccagc gacggctggc
      901 tggcggacgt gctgctctcc ctcgcgtaca cgccgcacct gggcgatccc gacggtccgg
      961 cgctgctagc cggcgacccg gcgcttcgtc accggttcag cgcggagaac cccgtcgccg
     1021 gccagcgcgc cgcggcgcgt tggcgcattc ccgtcgagac gctcggggaa gccgggggat
     1081 ggcgcgccac gggttccgtc ctgggcctcg acgtcggatt ggcggcgctc tcactgcgca
     1141 ggctgaccac cgacagcctg ccgccgccgc cgtcggtcaa cgagatcgac cggtcctcga
     1201 tcgcggccac gctggtcctc acgaatgcgt tcgatgtgac cgacgcgggc cgcgacgcgc
     1261 tggcgggggc catcgcgcgc ggccgggcgc gcgtggaggc gctcgcgaac gattcgtccg
     1321 gcgtggccga cgtcgcgcag gcgtcgggca tcggcgagtg gcggcgccag atcctcccgt
     1381 gggcgctgcg gcacgagccg gcgcgcgcgg ccgagtactt ctcgctgtcg gaattcgcgc
     1441 gtctcggctc tgcccagccg gctcggcagc cggacctgga cgcatggggc acgtcggccc
     1501 tagccaggga aggctgcctc tgtctgcgct acgcggagcc ggggggatgg gagacgcttg
     1561 ccggccggtt cggcacagcg cttgtggccg agcagatgcc ggacctgtcg cttcgcatcg
     1621 cggagtcgct cgccgggctg ggactgccgg cgcggctgct gcccgccgtg ctggctttcg
     1681 tggcacagga cgtcctcgac acctgcgccc cggcccacct cgacgactgg accgcacttg
     1741 ccgccgccgt gcggcgcctg cccgactccc gcatcgcgga tgacatcgcc gcgctcacgg
     1801 ctggcgggcc gcttgtgccg gcggaccggg agtggcgcga tgatgcgcgg cgctgacgtc
     1861 ctcgcgatcg ggccgcgcca gccgcggcgc gcgtggctgc tcgcggcggc gctcgcggcc
     1921 gcggggctcg cggcgcaggc cccggagccg tcggtcgtgt tcctgtctcc ccacagcggt
     1981 gactacacca gcggccccac gcccattcgc gtccaagtgg acccgcctgg cacggccgtc
     2041 tcgagtctca gcctgttcgc agacggcagg ctcgtctgca cgctggacca cccgccgttc
     2101 gagtgcgcct tcgacgccgg tccgaacgtc gtcgagcacg cgctgcgcgc cgtggcgacg
     2161 ctgccgggcg gccggcgcat cgcccagtcg gtgcggacgc aaggcgtcgc ctacacggag
     2221 agcgtggacg tggacgtcgt gcaggtcacg gtcaccgtca ccgacggcgc cgggcgattc
     2281 gtccgcggcc tgacgcggca ggacttccgc ctgttcgaag acaacgttcg gcaggacgtg
     2341 acgacgttca tcgccgagaa cgtgccgacc gagctcatcg tcgcggtgga tgtcagcggg
     2401 agcatgacgg acgccatgcc gaacgtgaag gagtccgtca agcggttcct gtcggcgatt
     2461 cggccgaccg accgggtcac cgtcctcggc ttcaacgaca gcgtgttcac cctggcccgg
     2521 ccgacggtcg atctcgcagc gcggctgcgc gccatcgacc ggctcgcgcc ctggggcggc
     2581 accgcgctgt acgacgtgat catccaggcg ttcgatctgc tgggccgcca aaccgggcgg
     2641 cgggcgctcg tcgtcttcac cgacggcgag gacctcaaca gccgcgcgcc catcgagacg
     2701 gccgagcggc ggctggaagc gagcgacgcc gtgatgtacc ccatcggcca gggccgggcg
     2761 cagaagctcc agagcctgcg cgccgtgctc gagcggctgt ctcgcaagag cggcggccgt
     2821 gcgttcttcg gcgacgacgt cgagaagctg gatggcgtct tcggcgacat cctcgaggag
     2881 ctgtcgaacc agtacctcct cgggtacgtg ccgagggacc tcaggcgcga cgggcgctgg
     2941 aggatcctga aggtcgaagt gcccgggaag gagcttcgcg tccgggcgag acaggggtac
     3001 cgggcggagc cgaagaagta ggcgccgcgc gcgcggcggg cgagaggagc ggcgatggcg
     3061 aatcgggccg gggtggcgtg cgcggctgtg gcgctggcgg ctctcctgca ggggccgctg
     3121 gtgccggtta ccgcgctggc gcagcagccg ccctccttca agtccggcgt ggacgtcatc
     3181 gcggtggacg tcagcatcgt ggacaaagag ggccagccgg tcggcgggct cgacgccgat
     3241 cggttcgagg ttgccgtcga cggcaagccc cgccgcgtcg tgtcggcgac cttcgtggac
     3301 tacgcgtccg cggcagccgg cgcaggctcg gcgccgctca ccggacgccc gcttcagctc
     3361 ggcgcgagct tcgcctcgaa cgagcgcgag gaggagccgt cggccatccc cgggcgcgtc
     3421 gtcgtgctgg ccgtggacca gttgagcttc gcgccgggcg cggcccgcgc cgccatcgag
     3481 agcgcgcaga ggttcctcga ccgcctgcag ccggccgacc gcgtcggtgt cgtcgcctat
     3541 ccggcgccag gccccgtggt ggcgccgacg gtcgagcacg gcgtcgtgcg cgccgcgctg
     3601 gagcgggtcg tggggctcgg cgagacgggg cagcccgtgg cgccgtacct cagcatgtcc
     3661 gaggccatgg cgctggaccg caacgacccg caggtgcgcc agcaggtgat cgagcgcgag
     3721 tgcgccggcc tgaagcagcc cgccattcgg ggcgcgttct cggaaatcga atcctgcgtc
     3781 cgccgcatcg acggcctggt gccgcagatg ctcatgcagc tccagacgca aggcgcgcgg
     3841 agcctcaacg ggctccagtc cgtcgtcaac gacctggacc gcatttccgg cgtgaagacg
     3901 ctgatcgtga tcagcgccgg catgctgccc agctcgtctg ctcgcctcga tcttcggcgc
     3961 gacatccagg cggtggcggc cgcggcggcc tccgccaacg tccgcatcta cgtgctgcac
     4021 gtccagaggg agttcctcca ggcggtttcc gccgagatgg cccggccgtc gccaaccgtg
     4081 ttcgacaacg agatcgtgct cgcctccggg ctgcagatgc tggcgggcct gagtgccggg
     4141 tcgatcttca ccgtcgcctc cggcgccgac cgggcgttcg accgcgtcgc gcgggagtcg
     4201 tcggcggcct acatcctcgg catcgagccg gaagctggcg atcgggacgg gaagtcgcac
     4261 cggatccagg tgcgcgtgcg gaacctgccg aacgctttgg tccggagccg ctcgtccttc
     4321 gtggtgcccg cgccgtcggc cgctccaccc acgccggagg cggccgttac cgccgcactg
     4381 aagccggggc ggctcgcaaa ggaccttccg gtgcagttca ccgcgcatgc gatgcgcgac
     4441 ccgagaaccg gagagattcg cctcctcatg tcggcgaaca tcggccgtgg cgtagccggc
     4501 ccggccgagg tccgcgtggg ctacgccgtc agcgaccaga ccggccgggt actgggatcc
     4561 gccgtcgaca agctccacct cgatcagcgg ggggctgggc cgtcggcgtc ttggtcgtat
     4621 ctgaacgcga ttggcctcag gcccggcgcc tacaccctcc gcgtcgcagc tgccgacgaa
     4681 ggggggagga ccggcagcgc ggagtacagg atcgatgcga agctgaccg
//
LOCUS       DAOSIF010000219         4727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16068_length_4727_cov_3.357877, whole genome shotgun sequence.
ACCESSION   DAOSIF010000219 DAOSIF010000000
VERSION     DAOSIF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16068_length_4727_cov_3.357877"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3334)
                     /locus_tag="PLT35_08210"
     CDS             complement(<1..3334)
                     /locus_tag="PLT35_08210"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29249.1"
                     /translation="MYSLPKNVYRERIDFSKIKATVPIPNLIEIQRKSYERFLQMDKL
                     PDEREDIGLQSVFKSVFPISDFRENSSLEFIEYSIGNWECKCGRLQGLQHMRKPCTNC
                     GHLLVADPYGERDVLCPRCGRANEARGEVCDICGTTVRLKDKYDVAECQERGMTYAVP
                     LKVTIRLVVWNKDPDTGAKTIRDIKEQEIYFGDIPLMTDHGTFIINGTERVIVSQLHR
                     SPGAFFHSEDKALYVAQVIPYRGSWVEFEYDAKNLLYVRIDRKRKFLATVFLRALGLR
                     GADEVLRTFYAIDRIHLGPGGAYWDVADSLLGLRAGEDIAVPGSEIVIAKGRRISPTH
                     LRALRAAGVARVKIDEAEREGAFAAADVVDPETGEIIVEAGEEVTHRVIAIAQEKGVG
                     RIEVFFPERDEVGPTIAATLKKDPVRTHEDALVEIYRRLRPGDPPTLDSSRSLFENMF
                     FNPQKYDFSRVGRLKLNTKLGLTTPLEEKILHPQDFYEVIRYLLRLRKNPALVDDIDH
                     LGNRRVRSVGELLENQFRIGLVRMERAIKEKMSVYQEMATAMPHDLINAKPVMAAIRE
                     FFGSSQLSQFMDQTNPLSEVTHKRRLSALGPGGLSRERAGFEVRDVHTTHYGRICPIE
                     TPEGPNIGLISSLASYARINEFGFIESPYRKVQDGQIVDYALITNAGASRHKVGDVVP
                     LADILEESGGKRRVKKGIEYEPHPFYLSAWEEDQYVIAQANVAVGEDGRLLEDRVTAR
                     QEGNFILAQRDAVQFVDVSPKQLVSVAASLIPFLEHDDANRALMGSNMQRQAVPLLRA
                     RAPFVGTGMEYKTATDSGACITAKRSGTVDYVDSQRIVVRVAGEEDNVSREMGADIYP
                     LTKFKRSNQNTCINQKPIIRVGQRVEKGQVLADGPCTDLGELALGRNVLVAFMPWRGY
                     NFEDAILVSERMVKDDYYTSIHIEEFEIESRDTKLGPEEITRDIPNVSETFLRDLDES
                     GIIRLGAAVKAGDLLVGKVTPKGETQLTPEEKLLRAIFGEKAGDVRDASLICPPGVEG
                     IVVGVRIFSRKGIEKDERAKAIEAEELELMEKNLQDEVRILHDEVKKRATRIVLGQTL
                     RSDLFDDDGRERLLRKGTILTAE"
     gene            complement(3470..3862)
                     /gene="rplL"
                     /locus_tag="PLT35_08215"
     CDS             complement(3470..3862)
                     /gene="rplL"
                     /locus_tag="PLT35_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOG29250.1"
                     /translation="MAELTQSQVVEYIKHISVVELSQLVKALEQELGVSAAAAIPMAM
                     PVGGAAAGAAAAPAEEKTEFDVVLTDFGAKKIEVIKAVREVTSLGLKEAKDLVEGVPK
                     VVKEGVPKAEAEAIKKKFEDAGAKAEIK"
     gene            complement(3891..4415)
                     /gene="rplJ"
                     /locus_tag="PLT35_08220"
     CDS             complement(3891..4415)
                     /gene="rplJ"
                     /locus_tag="PLT35_08220"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG29251.1"
                     /translation="MALTRSEKQTELAALETELRAAESAILVDFKGLNVPQVTELRRQ
                     VRAAKGRYRVVKNTLAKRALKGTSFAPLTEQCAGATALAYTGGDPVALAKVLTAFIKT
                     APALSIKGAVVQGRAIPPGEVAELAALPGRPELCARLLYVLQAPMVQLVSVLNAAPRD
                     LVGVLAAAEKKRSA"
     gene            complement(4440..>4727)
                     /locus_tag="PLT35_08225"
     CDS             complement(4440..>4727)
                     /locus_tag="PLT35_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG29252.1"
                     /translation="PKTGTVTPDVAKAVREIKAGKVEFRVDKTGIVHAPVGKVSFAAD
                     RLIENARALVESLLRAKPAAAKGKYMKSVTICSTMGPGVRVDTAGLEAARR"
BASE COUNT          632 a         1640 c         1617 g          838 t
ORIGIN      
        1 cctcggcggt gaggatcgtg cccttccgca gcaggcgctc gcggccgtcg tcgtcgaaga
       61 ggtccgagcg cagcgtctgg cccagcacga tccgcgtcgc ccgcttcttc acctcgtcgt
      121 ggaggatgcg gacttcgtcc tgcaggttct tctccatcag ctcgagctct tcggcctcga
      181 tggccttggc ccgctcgtcc ttctcgatgc ccttgcggga gaagatcctc acgccgacga
      241 cgatgccctc gacgcccggc gggcagatga gcgacgcgtc acgcacgtcg cccgccttct
      301 cgccgaagat cgcccgcagc agcttctcct cgggggtgag ctgggtctcg cccttgggcg
      361 tgaccttccc cacgagcagg tcgccggcct tgacggcggc gccgagccgg atgatgccgc
      421 tctcgtcgag gtcgcgcagg aaggtctccg acacgttcgg gatgtcccgc gtgatctcct
      481 cggggccgag cttggtgtcc cgggactcga tttcgaactc ctcgatgtgg atggaggtgt
      541 agtagtcgtc cttcaccatc cgctcggaga cgaggatcgc gtcctcgaag ttgtagccgc
      601 gccagggcat gaaggcgacc agcacgttgc gcccgagggc gagctcgccc aggtccgtgc
      661 acggcccgtc ggcgaggacc tgccccttct cgacgcgctg gccgacgcgg atgatcggct
      721 tctggttgat gcaggtgttc tggttcgagc gcttgaactt cgtcaggggg tagatgtcgg
      781 cccccatctc gcggctgacg ttgtcctcct cgccggcgac gcgcacgacg atgcgctggg
      841 agtcgacgta gtccaccgtg ccggagcgct tcgcggtgat gcaggcgccc gagtcggtgg
      901 cggtcttgta ctccatgccc gtgcccacga agggggccct ggcgcgcagg aggggcacgg
      961 cctggcgctg catgttcgag cccatcagcg cgcggttcgc gtcgtcgtgc tcgaggaacg
     1021 ggatgagcga ggccgccacc gagacgagct gcttcggcga cacgtccacg aactgcaccg
     1081 cgtcgcgctg cgcgaggatg aagttgccct cctgccgcgc ggtgacgcgg tcctcgagca
     1141 gcctgccgtc ctcgccgacg gcgacgttgg cctgggcgat gacgtactgg tcctcctccc
     1201 acgccgacag gtagaagggg tgcggctcgt actcgatgcc cttcttgacc cggcgcttcc
     1261 cgccggactc ctccaggatg tcggcgagcg gcacgacgtc gccgaccttg tgcctgctgg
     1321 cgccggcgtt cgtgatgagc gcgtagtcca cgatctggcc gtcctggacc ttgcggtagg
     1381 gcgactcgat gaagccgaac tcgttgatgc gcgcgtacga ggccagcgac gagatcaggc
     1441 cgatgttcgg gccttccggc gtctcgatgg ggcagatccg gccgtagtgc gtcgtgtgga
     1501 cgtcgcggac ctcgaagccc gcccgctcgc gcgacagccc gccaggcccg agggccgaga
     1561 gccgccgctt gtgggtgacc tcggagaggg ggttggtctg gtccatgaac tgcgagagct
     1621 gcgaggagcc gaagaactcc cggatcgccg ccatgacggg cttggcgttg atcaggtcgt
     1681 gcggcatggc cgtcgccatc tcctggtaga ccgacatctt ctccttgatg gcgcgctcca
     1741 tgcggaccag cccgatgcgg aactggttct cgagcagctc gcccaccgag cggacgcggc
     1801 ggttgccgag gtggtcgatg tcgtccacga gggcggggtt cttgcgcagc cggaggaggt
     1861 agcggatcac ctcgtagaag tcctgcgggt gcaggatctt ctcctcgagg ggcgtggtga
     1921 ggccgagctt ggtgttgagc ttgaggcggc ccacccgcga gaagtcgtac ttctgcgggt
     1981 tgaagaacat gttctcgaac agcgagcgcg agctgtcgag ggtcggcggg tcgcccgggc
     2041 ggaggcgccg gtagatctcg acgagcgcgt cctcgtgggt gcggacgggg tccttcttga
     2101 gcgtggcggc gatggtcggc ccgacctcgt cgcgctcggg gaagaagacc tcgatgcgcc
     2161 cgacgccctt ctcctgcgcg atggcgatca cccggtgggt gacctcctcg cccgcctcga
     2221 cgatgatctc accggtctcc gggtccacca cgtcggcggc ggcgaaggcg ccctcgcgct
     2281 ccgcttcgtc gatcttcacg cgcgcgacgc cggccgcgcg cagggcgcgc aggtgggtcg
     2341 gggagatcct gcgccccttg gcgatcacga tctcgctgcc cggcacggcg atgtcctcgc
     2401 cggcgcggag gccgaggagc gagtcggcca cgtcccagta ggccccgccc ggcccgaggt
     2461 ggatgcggtc gatggcgtag aaggtccgga gcacctcgtc ggcgccgcgg aggccgaggg
     2521 cccgcaggaa gacggtcgcg aggaacttcc gcttgcggtc gatgcggacg tagaggaggt
     2581 tcttcgcgtc gtactcgaac tcgacccagg agccgcggta cgggatgacc tgggcgacgt
     2641 agagggcctt gtcctcggag tggaagaagg cgcccggcga gcggtggagc tgggagacga
     2701 tgacccgctc ggtcccgttg atgatgaacg tgccgtggtc ggtcatcagc gggatgtcgc
     2761 cgaagtagat ctcctgctcc ttgatgtcgc ggatggtctt ggcgcccgtg tccgggtcct
     2821 tgttccagac gacgaggcgg atggtgacct tgaggggcac ggcgtaggtc atgccgcgct
     2881 cctggcactc ggccacgtcg tacttgtcct tgaggcggac cgtcgtgccg cagatgtcgc
     2941 acacctcgcc ccgggcctcg ttggcccgcc cgcagcgcgg gcacagcacg tcgcgctcgc
     3001 cgtaggggtc ggccacgagc aggtggccgc agttcgtgca cggcttgcgc atgtgctgca
     3061 ggccctggag cctgccgcac ttgcactccc agttgccgat ggagtactcg atgaactcca
     3121 gcgacgagtt ctcccggaag tcgctgatcg ggaacacgga cttgaacacc gactggaggc
     3181 cgatgtcctc ccgctcgtcc gggagcttgt ccatctgcag gaaccgctcg tacgacttcc
     3241 gctggatctc gatcaggttc gggatcggca ccgtcgcctt gatcttggag aagtcgatgc
     3301 gctcgcggta gacgttcttc ggaaggctgt acatgctgct ctcgggacta gggcctgggg
     3361 gccatccaac acgcgggcac gggcggacgc ctcgcgtgtc aaccacgcat cgggcacgtc
     3421 cgcggcgcgc gcccccggcc ggacgcgcgc cgcgtcaccg gaacggcggc tacttgatct
     3481 cggccttcgc gccggcgtcc tcgaacttct tcttgatcgc ctcggcctcg gccttcggca
     3541 cgccttcctt gacgaccttc ggcacgccct cgaccaggtc cttggcctcc ttcaggccga
     3601 ggctggtgac ctcgcggacg gccttgatga cctcgatctt cttcgcgccg aagtcggtca
     3661 gcacgacgtc gaactcggtc ttctcctcgg ccggcgccgc cgccgcgccc gccgccgcgc
     3721 cgcccaccgg catggccatc gggatcgccg cggccgccga cacgccgagc tcctgctcga
     3781 gcgccttgac aagctgcgac agctccacca ccgagatgtg cttgatgtac tccaccacct
     3841 gcgactgcgt cagttcggcc attgctcgct ttctcctctg ccagtggacg ttatgcgctc
     3901 cgcttcttct ccgccgccgc cagcacgccg accaggtcgc gcggcgcggc gttcagcacg
     3961 ctcacaagct gcaccatcgg ggcctgcagc acgtagagca gtctcgcgca gagctcgggc
     4021 ctgcccggca gggccgccag ctcggccacc tcgcccggcg ggatcgcccg gccctgcacc
     4081 acggccccct tgatgctcag cgcgggggcg gtcttgatga acgccgtcag caccttcgcc
     4141 agcgccaccg ggtcgccgcc cgtgtaggcc agcgccgtgg cgcccgcgca ctgctccgtc
     4201 agcggcgcga acgaggtgcc cttcagcgcg cgcttcgcga gggtgttctt caccacgcgg
     4261 tagcggccct tcgccgcgcg cacctggcgc cgcagctcgg tgacctgcgg cacgttgagc
     4321 cccttgaagt cgacgaggat cgcgctctcg gccgcgcgga gctcggtttc gagcgcggcc
     4381 agctcggtct gcttctccga tcgtgtgagt gccatggccc tctgatcctg gttccgcggc
     4441 tagcgacgcg ccgcctcgag ccccgccgtg tccacccgga cgcccgggcc catcgtggag
     4501 cagatggtga cgctcttcat gtacttgccc ttcgccgccg cgggcttcgc gcgcagcagg
     4561 ctctccacga gcgcccgcgc gttctctatc agccggtccg ccgcgaacga caccttcccc
     4621 accggcgcgt gcacgatgcc ggtcttgtcc acgcggaact cgaccttgcc cgccttgatc
     4681 tcgcggacgg ccttcgccac atcgggggtc accgtgccgg tcttcgg
//
LOCUS       DAOSIF010000220         4714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16125_length_4714_cov_3.344709, whole genome shotgun sequence.
ACCESSION   DAOSIF010000220 DAOSIF010000000
VERSION     DAOSIF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16125_length_4714_cov_3.344709"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLT35_08230"
     CDS             <1..774
                     /locus_tag="PLT35_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOG29253.1"
                     /translation="AINVNDSVTKSKFDNIYGCRHSVVDGLNRASDVMLGGKVAVVCG
                     YGEVGKGCAQALRGQGARVVVTEIDPICALQAAMEGYQVATVEDVVSTADIFITATGS
                     YNVITAEHMSMMKDKAIVGNIGHFDNEIDMAGLKKVPGIQRINIKPQYDEWRFPDGHS
                     VMILAEGRLLNLGCATGHPSFVMSASFSNQVLAQLELHLHNEQYERKVYMLPKRLDEE
                     VARLHLPHLGVKLTTLTKEQADYLGIPIEGPYKAEHYRY"
     gene            850..1770
                     /locus_tag="PLT35_08235"
     CDS             850..1770
                     /locus_tag="PLT35_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HOG29254.1"
                     /translation="MIGALSPFAPVLLALSATAVAVPEPAFAEEAKSRTAVEQLIRGS
                     GADVAVAFRTLDAPAGSPARLELLIQPDAEFHAASTMKVPVMIELFRQAREGLVRLDE
                     PLEIRNAFTSIVDGSPYALSADDDSDAAIYARAGQSMTLADLCEPMITVSSNFATNLL
                     IQRLDPARVQRTVRDLGADGMIVRRGVEDQKAFDAGLNNSTTARALMVLMERIARLEA
                     VDADASRRMLDILTRQRFNEGIPALLPPGTPVAHKTGSITRINHDAAVVLAPRPFVLA
                     VLVRGVDDHARSAALIAQIARVLYDESQAR"
     gene            1802..3715
                     /locus_tag="PLT35_08240"
     CDS             1802..3715
                     /locus_tag="PLT35_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOG29255.1"
                     /translation="MRRRARGGWWLAGIAAIVALGAAAVGAQRGQAPASVQAAYTKHE
                     YRIPMRDGVRLFTAVYVPRDRSAVWPILLTRTPYGVAPYGPDAYKSSLGPSSPEFLRS
                     GYIFVYQDVRGRMMSEGEFVNMRPHLAKKAGPADIDESTDAYDTIEWLLRNVPGHNGR
                     VGMWGISYPGFYASAGMIDAHPALKAVSPQAPITDWFIGDDFHHNGALYLPHAFGFLS
                     GFGRPRPAPTTQSGSRFDFGTEDGYRFYLTLVPLSNANALYLKDTVAFWNEMMAHETY
                     DAFWQARNLRPHLRRIKPAVLTVGGWFDAEDLFGALETYAHVERNSPGATNTLVMGPW
                     AHGGWTGDGDFLGDLRFGSRTGRFFQEHIEFPFFEHHLKDAPDPGLPEACVFETGRNQ
                     WRRLDAWPPAEAAAKALYLRPGGRLSWQPPSEASAFDEYISDPARPVPYINHLAIGMT
                     REHMVDDQRFASRRPDVLTYATDVLDSDVTVAGPIRPSLQVSTTGTDSDWVVKLIDVH
                     PESAAGPSSSGERALGLRPASRMAGYQQLVRGEVMRGKFRDSYEAPQPFVPGEVTRVE
                     YVVPDVFHTFRAGHRIMVQIQSTWFPLVDLNPQTFTNIPAAAKGDFRKATQRVYRGQR
                     AASHIVLSVLEGP"
     gene            complement(3822..>4714)
                     /locus_tag="PLT35_08245"
     CDS             complement(3822..>4714)
                     /locus_tag="PLT35_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29256.1"
                     /translation="FKLLNDLYGQDKIKEWLRQDIALWRQNDTEALPMGYLLCGPVGT
                     GKTFMVRCLAGEAGVPVVKIKNFRDKWVGSSEGNLEKILSVIKAIGQVVVIVDEGDRA
                     FGSTESEGDGGTSSRVIARIKEFMSDTSNRGRILFLVMTNRPDKLDVDLKRAGRLDRK
                     IPFLYSQSAEEVEQVARAVIRKNRIATDVDIASIRGTFSARLVGYSNADVEAVVLMAN
                     DDAAREAGGEATVEGRHFERAAADYFPSRDAELLEYMELLAVFEASSRRLLPPKYASM
                     TPEELDLRLRTLRLAVGSRR"
BASE COUNT          758 a         1619 c         1625 g          712 t
ORIGIN      
        1 gccatcaacg tcaacgactc ggtgacgaag agcaagttcg acaatatcta cggctgccgc
       61 cactcggtcg tcgatgggct gaaccgggcc agcgacgtga tgctgggcgg caaagtcgcg
      121 gtcgtgtgcg ggtacggcga agtggggaag ggctgcgccc aggcgcttcg cggccagggc
      181 gcgcgcgtgg tggtgaccga gatcgatcca atctgcgccc tgcaggcggc gatggaaggc
      241 taccaggtgg cgactgtcga agacgtcgtg tcgacggcgg acatcttcat cacggcgacc
      301 gggagctaca acgtcatcac cgccgagcac atgtcgatga tgaaggacaa ggccatcgtc
      361 gggaacatcg gccacttcga caacgagatc gacatggccg gcctgaagaa ggtgccgggc
      421 atccagcgca tcaacatcaa gccgcagtac gacgagtggc ggttccccga cgggcacagc
      481 gtgatgatcc tggccgaggg gcgcctcttg aatctcgggt gcgccaccgg gcatcccagc
      541 ttcgtgatgt cggcgtcgtt ctcgaaccag gtgctcgccc agctcgagct gcacctgcac
      601 aacgagcagt acgagaggaa ggtatacatg ctgccgaagc gcctcgacga agaggtggcg
      661 aggctgcacc tgccgcacct cggggtgaag ctgaccacgc tgaccaagga gcaggcggac
      721 tacctgggca ttccgatcga gggcccgtac aaggcggagc attatcgata ctagcaaggg
      781 ggtcagatcc caagaaccgt gccattggca cggttcttgg gatctgaccc cgcgacgctg
      841 ggcatgcgct tgatcggagc actctcgccg ttcgcgccgg ttctgctggc gctctccgcc
      901 acggccgtgg cggtgcccga gccggcgttc gccgaagagg cgaagtcgag aacggcggtc
      961 gagcagctca tccgcggaag cggggccgac gtggccgtgg cattccggac ccttgacgct
     1021 cccgccgggt ccccggcccg cctcgaactc ctgatccagc ccgacgccga gttccacgcc
     1081 gccagcacca tgaaggtgcc ggtgatgatc gaactgttcc gccaggcgcg cgaaggcctg
     1141 gtgcgcctcg acgaaccgct cgagattcgc aacgccttca cgagcatcgt ggacggcagc
     1201 ccgtatgccc tcagcgcgga cgacgactcc gacgcggcca tttacgcgcg ggcggggcag
     1261 tccatgacgc ttgctgattt gtgcgagccc atgatcacgg tcagctcgaa cttcgcgacc
     1321 aatctgctca tccagcggct ggatccggcg cgcgtgcagc gcacggtgcg ggacctcggg
     1381 gcggacggca tgatcgtgcg gcgcggggtc gaggaccaga aggcgttcga cgcggggctg
     1441 aacaactcga cgacggcgcg ggcgctgatg gtcctcatgg agcggatcgc gcgcctcgag
     1501 gcggtggacg cggatgccag ccggcggatg ctcgacatcc tgaccaggca gcggttcaac
     1561 gagggcatcc cggcgctcct gccgccgggg acgccggtcg cgcacaagac gggcagcatc
     1621 acccggatca accacgatgc ggccgtcgtc ctagcgccga ggccgttcgt gctggccgtg
     1681 ctggtgcgcg gcgtcgacga ccatgcgcgc agcgccgcgc tcatcgcgca gatcgcccgc
     1741 gtcctctacg acgagtcgca ggcgaggtag cggagcggcg gccgggagag gagagcggag
     1801 gatgcgccgc agggcccgcg gcggatggtg gctggcgggg attgctgcca tcgtggcgct
     1861 cggggccgct gcggtcggcg cgcagcgcgg ccaggcgccg gcttcggtcc aggccgccta
     1921 caccaagcac gagtaccgca tcccgatgcg ggacggcgtg aggctgttca ccgccgtcta
     1981 cgtgcccagg gaccgcagcg ccgtctggcc gatcctgctc acccgcacgc cgtacggcgt
     2041 ggcgccctac gggcccgatg cgtacaagag cagcctcggc ccctcgtcgc ccgagttcct
     2101 gcggagcggg tacatcttcg tgtaccagga cgtgcggggc cgcatgatgt cggagggcga
     2161 gttcgtcaac atgcggccgc atctggcgaa gaaggctggc ccggcggaca tcgacgagag
     2221 caccgacgcc tacgacacca tcgagtggct gctgaggaac gtgcccgggc acaacggccg
     2281 cgtgggcatg tgggggatct cgtaccccgg cttctacgcg tccgccggca tgatcgacgc
     2341 ccacccggcg ctcaaggccg tgtcgccgca ggcgcccatc accgactggt tcatcggcga
     2401 cgacttccat cacaacggcg ccctctatct gccgcacgcc ttcggcttcc tgtcgggctt
     2461 cggacggccc aggcccgcac ccaccacgca gtccggttcc cggttcgact tcggcaccga
     2521 ggacggctac cggttctacc tgacgctcgt cccgctctcg aacgcgaacg ccctgtacct
     2581 gaaggacacc gtcgcgttct ggaacgagat gatggcccac gagacctacg acgcgttctg
     2641 gcaggcgcgc aacctccggc cgcacctgag gcgcatcaag ccggccgtgc tgaccgtcgg
     2701 cggctggttc gacgcggagg acctgttcgg cgcgctcgag acgtacgcgc acgtcgaacg
     2761 gaacagcccc ggcgcgacga acacgctcgt catggggccg tgggcccacg gcggctggac
     2821 cggcgacggc gacttcctgg gagatctgcg gttcggctcc aggacgggcc gcttctttca
     2881 ggagcacatc gagttcccgt ttttcgagca ccacctcaag gacgcccccg accccgggct
     2941 gccggaggcc tgcgtcttcg agaccgggcg gaaccagtgg cggaggctcg acgcctggcc
     3001 gcccgcggag gccgccgcga aggcgctgta cctccggccc ggcggcaggc tgtcctggca
     3061 gccgccctcc gaggcgtcgg ccttcgacga atacatcagc gatccggccc ggccggtgcc
     3121 ctacatcaac catctcgcca tcggcatgac gcgcgagcac atggtggacg accagcgctt
     3181 cgcctcgcgc cggccggacg tgctgacgta cgcgaccgac gtgctcgact cggacgtcac
     3241 ggtggccggc ccgatcaggc cgagcctgca ggtgtccacg accggcaccg actcggactg
     3301 ggtcgtcaag ctcatcgacg tccatcccga gagcgccgcc gggccatcgt cgtccgggga
     3361 acgcgcgctg gggctgcggc ccgcatcacg catggccggc taccagcagc tggtccgcgg
     3421 cgaggtcatg cggggcaagt tccgcgacag ctacgaggcg cctcagccgt ttgtgccggg
     3481 cgaggtgacg cgggtcgagt acgtggtgcc cgacgtcttc cacacgttcc gcgccggcca
     3541 ccggatcatg gtgcagatcc agagcacgtg gtttccgctg gtggacctga acccgcagac
     3601 gttcacgaac atcccggccg cggcgaaggg cgacttcagg aaggcgaccc agcgcgtgta
     3661 ccgcgggcag cgcgccgcat cgcacatcgt cctgagcgtc ctggaggggc cctgacgccc
     3721 ggacggccgc ggccgcctgg ctggcggcag ggccggcccc ggcgggccgc ggtccgcgcg
     3781 gcccaggacg gcgattggtc ccgggaggga ccgtggaacc gctaccgccg gctgccgact
     3841 gcgagtctga gcgtgcggag gcggaggtcg agctcctccg gggtcatcga cgcgtacttc
     3901 ggggggagca gccgccggct cgacgcctcg aacaccgcca gcagctccat gtactcgagg
     3961 agttcggcgt cccggctggg gaagtagtcc gcggccgccc gctcgaagtg ccgcccctcg
     4021 accgtcgcct ctccccccgc ctcccgcgcc gcgtcatcgt tcgccatcag cacgacggcc
     4081 tcgacgtccg cgttgctgta gccgaccagc ctggcggaga acgtcccgcg gatggaggcg
     4141 atgtccacgt cggtggcgat gcggttcttg cgaatgaccg cgcgcgccac ctgctcgact
     4201 tcctccgccg actgggagta caggaacggg atcttccggt cgagccggcc cgcgcgcttc
     4261 aggtccacgt cgagcttgtc cgggcggttc gtcatgacga ggaacaggat gcggccccgg
     4321 ttcgaggtgt ccgacatgaa ctccttgatg cgcgcgatca cgcgcgacga cgtgcccccg
     4381 tcgccctcgc tctccgtcga cccgaaggcc cgatcgccct cgtccacgat tacgacgacc
     4441 tggccgatgg ccttgatcac cgaaaggatc ttctcgaggt tgccctcgct cgaaccgacc
     4501 cacttgtcgc ggaagttctt gattttgacc acgggcacgc ctgcctcgcc cgcgaggcag
     4561 cggaccatga aggttttgcc ggtgcccacg ggaccgcaga ggagatagcc catggggagg
     4621 gcctcggtgt cgttctggcg ccagagggcg atatcctggc ggagccactc cttgattttg
     4681 tcctggccgt agaggtcgtt gagcagcttg aagc
//
LOCUS       DAOSIF010000221         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16140_length_4710_cov_3.096885, whole genome shotgun sequence.
ACCESSION   DAOSIF010000221 DAOSIF010000000
VERSION     DAOSIF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16140_length_4710_cov_3.096885"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLT35_08250"
     CDS             <1..270
                     /locus_tag="PLT35_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG29257.1"
                     /translation="EKFRQALVHRVYHLAADRVVALHKHPRHDEVFYCVKGAGYGVLE
                     SAEVPLTPGQAFIVPAGVMHALRTEGDLYVTSTLVPLADDPQFSL"
     gene            513..1880
                     /locus_tag="PLT35_08255"
     CDS             513..1880
                     /locus_tag="PLT35_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HOG29258.1"
                     /translation="MTGPTLEETRSDEGGTTSVTMSAGPSRGRMLGRIADYKIGSIPL
                     PLYLAMALIVVGAALQRRLPSDMIGGFAAIMIMGIPLGHIGARVPLLRSIGGPAILSL
                     FVPSALLGWKMLDPELAKAVTALMKTANFLYLYIACLVAGSMLGMNRRILIQGFARMF
                     VPLLVGSVASVAAGIATGSLLGYAPRDTFFYIVIPIVGGGIGEGILPLSIAYSEILHR
                     PQSELIALMIPAALIGNVVAITSSGLLKHLGEKRPHLSGRGLLVRTHDADVLVEEQNA
                     ERPIDLRLMGSGLLIACTFFILGSLLAPYVGIPGPILMILGAAACKVLRVVPASMEQG
                     AHQMYRFVSLNLTFPLLAGLGVVYVPWADLVGSFTIPYFCICAATVLAMVASGFFIGF
                     VMRMYPVEAAIVTACHSGLGGTGDVAILSASNRMGLMPFAQIATRVGGASMVVIAVFL
                     LKLLR"
     gene            2301..3410
                     /locus_tag="PLT35_08260"
     CDS             2301..3410
                     /locus_tag="PLT35_08260"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOG29259.1"
                     /translation="MRTTAARAAGWGSLETAPAAVLGGAAAQTPSGGAETAPRVALSG
                     DVRVRYEHTTTDEGIPERDRGVVRARLNAQYSLSPNIAFGLRVATGDLTDPRTTDVTL
                     GDFAHKLQANLDRVFVTVRGGPVALTAGRMPNPFVASELAWDGDVNPHGVAGELVLPK
                     MGRLTPSVTSMLFVVDELTAASDSTMLGTQVLGKLKLSPAATLTLGGAYYDYRMLTLS
                     KSDVRGNLLTADGKRYLSDFDLVSVLGRLTYGRPETKWPVTFLTEYIVNRGTAGGAND
                     DGFDVEIDVGRSRVKGAMRARYGYMTADRDAVLNAFSHDNIPLASGYRMHTFGWDWYQ
                     RKDAFVNVTLYVFRDTLVPSSPYHARLRLNFTVEF"
     gene            3466..4029
                     /locus_tag="PLT35_08265"
     CDS             3466..4029
                     /locus_tag="PLT35_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29260.1"
                     /translation="MEMRVLKMAVVAGAFVAATALATAATAQQEIPRAPSTPDTPKNW
                     VAPAYKSLAQALVDELMAKHPEVASITMHGTPPGKKDIYTMFAGSFDDRIGNESSGGD
                     IITIKKGVTQVESKWGSANWMKRVTIVAPVKNAKGDYLPIAMCIAFYQSPTSGKIDTD
                     FLQPGVKIRDSIMSRIASVEALFGPAK"
     gene            complement(4521..>4710)
                     /locus_tag="PLT35_08270"
     CDS             complement(4521..>4710)
                     /locus_tag="PLT35_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29261.1"
                     /translation="PLSRFVYVFVNRKPDQPLEPKVKEFLKAILSKEGQQQVSNDRVY
                     LPLTPKIVAEELAKLNQM"
BASE COUNT          771 a         1534 c         1615 g          790 t
ORIGIN      
        1 gagaaattcc gccaggcgct cgtccacagg gtgtatcacc tcgcggccga tcgggtggtg
       61 gcccttcaca agcatcctcg acacgacgag gtcttctact gcgtgaaagg cgcgggctac
      121 ggcgtcctgg aatcggccga ggttcccctg acgcccggtc aggccttcat cgtccctgcg
      181 ggtgtcatgc acgccctgag aacggaaggg gacctctacg tgacgtcgac cctggtgccc
      241 ctggccgacg atcctcaatt cagcctgtaa cggccagtga cccggcgtcc ggcgccgcgc
      301 cgctggcggg gagcgccttc gtggccggtg gaatcggcga tcgacggcga ggagcctgtg
      361 ctggcggtcg cgcggatctc cgggtgcagc gtggagcagc gcgcctgacg cgacgtccgg
      421 ttcaggaagg ccgtccgcga gccgatcgct gaagagaact tcagcgcgaa ttaaggtgcg
      481 ggcccgggat cgttccacta cgatgccccg acgtgaccgg tccaaccctg gaggaaaccc
      541 gctcggacga gggcggcacg acgtccgtga cgatgtccgc ggggccatcc cgcggcagga
      601 tgctcggccg aatcgccgac tacaagatcg ggagcatccc ccttcccctc tacctggcga
      661 tggcgctcat tgtcgtcgga gcggccttgc agaggaggct gccatccgac atgatcggcg
      721 gctttgccgc catcatgatc atgggcatcc cgctgggcca cattggcgcg agggttccgc
      781 ttctgcggag catcggcggc cccgccatcc tgtcgctctt tgttccgtcc gcgctgcttg
      841 gctggaagat gctcgacccg gagctggcca aggccgtcac cgcgttgatg aagacggcga
      901 acttcctcta tctgtatatc gcctgcctgg tggcgggaag catgctgggg atgaaccgca
      961 ggatcctgat ccagggcttc gcgaggatgt tcgtcccgct gctggtcggc tccgtggcct
     1021 ccgtcgcggc tggcatcgcc accggctcgc tgctggggta cgccccgaga gacacgttct
     1081 tctacattgt cattccgatc gtgggcggcg gcatcggcga gggcattctt ccgctctcga
     1141 tcgcctattc ggagatcctc caccggcccc agtccgagct gatcgcgttg atgatcccgg
     1201 ccgcattgat tggcaacgtc gtcgcgatca cgtcgagcgg tctgctgaag cacctcgggg
     1261 agaagcgccc gcacctctcg ggacggggcc tgctcgtccg cacccacgac gccgacgttc
     1321 tggtcgagga gcagaacgcc gagaggccga tcgacctgag gctcatgggg agcgggctgc
     1381 tgattgcgtg caccttcttc attctcgggt cgctcctcgc gccgtacgtc ggcatacccg
     1441 gcccgattct gatgatcctg ggagcggctg cgtgcaaggt gctgagagtc gttccggcca
     1501 gcatggagca aggcgcccac cagatgtaca ggttcgtgtc tctcaacctg acctttccgc
     1561 tgctcgccgg cctcggggtc gtgtacgtgc cgtgggccga cctcgtcggg tcgttcacga
     1621 tcccctactt ctgcatctgc gcggccacgg tgctggccat ggtcgcctcg ggcttcttca
     1681 tcgggttcgt catgcgcatg taccccgtgg aggccgccat cgtcacggcc tgtcacagcg
     1741 gcctcggcgg caccggtgac gtcgccatcc tctcggcgtc caaccggatg ggcctcatgc
     1801 cattcgcgca gatcgccacc cgggttggcg gcgcgtcgat ggtcgtcatc gcggtcttct
     1861 tgctgaagct gctgcgatga tccggccgtg gcgcggcccg gccagcgccg cgttgtggcc
     1921 atgagacccg aggcaggaga gagacgatcg tccggcggtg atgggcggca gttgaatggg
     1981 cgagataacg tattgacgtc aaacaggcgg cattcgttcc ccgttgcgag catgaagaat
     2041 aattcaccaa acgttcagga acgtgccggc gggcggttta tatgatcggc ccgtcctgtg
     2101 cggaatcccc gggcaggcag tcctaacggg ggtgtctcat gcattgcaag atcgttctgt
     2161 tggcagtcgt attgtgtgcg ggattggcgc ttcccgctca tgctcagcag gatgaaggga
     2221 agaacgtagc gcacccgtcg tcccaggccg tcgagacgct gacggacgaa cgggcgccaa
     2281 gtcagggccc gacggcgggg atgcgaacga cggcggctcg tgccgccggc tgggggagcc
     2341 tcgagacggc gccggcggcc gttctgggcg gcgccgcggc ccagacgccg agcggcggcg
     2401 ccgagaccgc gccccgtgtt gcgctctccg gtgacgtccg cgtccggtac gagcacacca
     2461 cgacggacga gggcattccg gaacgcgaca ggggggtcgt gcgcgcacgc ctgaacgccc
     2521 agtactcgct ttcgccgaac atcgcgttcg gcttgcgcgt cgcgacaggc gacctgacgg
     2581 atcccaggac gacggacgtg acgctggggg acttcgcgca caaactccag gcgaacctcg
     2641 atcgggtctt cgtcaccgtg cgtggggggc cagtggcgct gacggccggg cgcatgccca
     2701 acccgttcgt cgcctcggag ctggcgtggg acggcgacgt gaacccgcac ggggtggccg
     2761 gcgaactcgt gctcccgaag atgggccgcc tgaccccgtc ggtcacgagc atgctcttcg
     2821 tcgtcgacga gctgactgcc gcttccgact cgacgatgct cggcacgcag gttctgggca
     2881 agctgaagct gtcgccggcc gcgacgctga cgctgggcgg cgcttactac gactaccgca
     2941 tgctcacact gagcaagagc gacgtccggg ggaacctgct gacggcggac ggcaagcgct
     3001 acctctcgga cttcgacctg gtgtccgtgc tggggcgcct gacgtacggc cgcccggaga
     3061 cgaagtggcc cgtgaccttc ctgacggagt acattgtgaa ccgcgggaca gccggtgggg
     3121 cgaatgacga cggattcgac gtcgagatcg acgtcggccg ctcgcgcgtc aagggcgcga
     3181 tgcgggcccg gtacggctac atgacggccg atcgggacgc cgtgctcaac gcgttctcgc
     3241 acgacaacat cccgctggcc agcggctacc ggatgcacac gttcgggtgg gactggtacc
     3301 agcgcaagga tgcgttcgtc aacgtgacgc tgtacgtgtt tcgcgacacg ctcgtgccgt
     3361 ccagccccta ccacgcccgt ctccgtctga atttcacggt ggagttctga gcgcagcggc
     3421 aggagaagcg agcctggtga aggccgacga gaggaggagg aagtcatgga gatgagagtg
     3481 ctgaagatgg cggtggtggc gggcgcgttc gtcgccgcga cggcgctggc gacggcggcg
     3541 acagcgcagc aggaaatccc gagggcgccg tcgacgcccg atacgcccaa gaactgggtg
     3601 gctccggcgt acaagtcgct ggcacaggcg ctcgtcgacg agctcatggc caaacacccc
     3661 gaagtggcga gcatcacgat gcacgggacg cctccgggca agaaggacat ctatacgatg
     3721 ttcgccggct ccttcgacga ccggatcgga aacgagtcct ctggcggaga catcatcacg
     3781 atcaagaagg gcgtcaccca ggtggagtcg aagtggggct cggccaactg gatgaagaga
     3841 gtcaccatcg tggcgccggt caagaacgcc aagggagact acctcccaat cgcgatgtgc
     3901 attgccttct accagtcacc gacgagcggc aagatcgaca ccgatttcct gcagcccggc
     3961 gtgaagatac gcgacagcat catgagccgc attgcgagcg ttgaggcgct gttcggcccc
     4021 gccaagtaac cgcccagcac gccctgcgcg aggcagggcc cggaagaggc cgccgcggcg
     4081 ccacgccgcg gcggcccgcc ggcaccggat gatgcggcca ggttctcctg ccgcgaacgg
     4141 ttctcctgcc gcgaacggtt cccctgccgc gaacggttct cctggtcgtt taggctgcct
     4201 ccggctgtgg cagtagaacg gggtaggcgc aggcgctcct cgcgccgcgc ccgcaggaga
     4261 ggctgttctg ctcgctgacg cagcctccgg ctgagggagc ataacggcgc gggcgcaggc
     4321 gctcctggcg ccgcgcccgc aggaaaggct gcgccgtgcg agcgactcga cgtggctgag
     4381 ccggccggag cgagccgccg ctcgagaggc ccacggccac gcgccggaag gcagccgcgc
     4441 gccgaccgca ttccgagcgg ccgcgatgga tccggcggcg gccgagggcc cgcgagacgc
     4501 cggaacgaga acaccgcccg ctacatctgg ttcagcttgg cgagctcctc cgccacgatc
     4561 ttcggagtga ggggcaggta cacgcgatca ttgctcacct gctgctgacc ctccttgctg
     4621 agaatcgcct tcaggaactc cttgaccttg ggttcgagcg gctgatcggg cttgcggttg
     4681 acgaacacgt agacgaagcg gctcagcgga
//
LOCUS       DAOSIF010000222         4704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16179_length_4704_cov_4.126909, whole genome shotgun sequence.
ACCESSION   DAOSIF010000222 DAOSIF010000000
VERSION     DAOSIF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16179_length_4704_cov_4.126909"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLT35_08275"
     CDS             complement(<1..448)
                     /locus_tag="PLT35_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29262.1"
                     /translation="MRPDPALLSSVFTGCMLLAAFFAIGRTRLTTFLRHYAASSACLA
                     GVILVSAAVSGNPAEYAGAAATLVLKVVLIPLGLLRTARKLGDAMRLTSMTRPVLSYT
                     LAAASVAGAWLLARRLPLSAASGRAEGGELPLAGLLFVALALILLGF"
     gene            complement(445..1389)
                     /locus_tag="PLT35_08280"
     CDS             complement(445..1389)
                     /locus_tag="PLT35_08280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOG29263.1"
                     /translation="MSPALLTLVQGAIVVALAPLAPGLVRRFKAVLQGRQGPPTLLPY
                     WTIATLFRKETVVSQSASWVFLAAPTAVLASSLLAACVLPLVSRGGALAPLSHLLAVS
                     GIWMLGSVFLVLGGLDPGSAFGGMGASREMTISAFLEPALVVSLAALAIASGSPAVDA
                     MMDVAGRGLLLRPWLLPAVGGLALVALGENARYPVDNPATHLELTMVHEAMLLEYSGP
                     HLAILELASAVKLATFALLLANLLVPAGLLRAGAGPLAAAAAVPAAIAKLAVTMGGLA
                     LVESGMAKLRLYRLPEFFFGALFLGLTSLGIGLMAGFK"
     gene            complement(1386..3407)
                     /locus_tag="PLT35_08285"
     CDS             complement(1386..3407)
                     /locus_tag="PLT35_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG29264.1"
                     /translation="MTAGQAVAAGLPGAALLWVFAAIAGLAARGPRVRQLAHALLAAG
                     SASGLLASLAHQLAALPPLKLAAPPILFGAPFVVDRLSAFFLMLLHGVSLMAAWFGWR
                     YGEDEAAKYPPRLVVPLTAVFCLGMHGVLLSSGAASFLLFWEAMSVSSFFLVMADGTA
                     DSRKAALLYLAVAQAGALALLVGIGVLGGGSARVTLAALGTGLPALPPASRTLALGLV
                     AFAFTSKAGLAPFHAWLPEAHPRAPSQVSALMSGVMLKIAVYGLLRFTSICWPALPDG
                     WSLALAALGLGGAVVAVISANIARDIKRILAWSSVENLGLVFAMFGFSALAGAHGHPR
                     LASAILGAMFLHVFAHALFKAGLFLGAGAVLHGAHTAKIEDLGGLANRMPRLSAAMLA
                     LALAAAPLPPFGAFAAEWLLVESLLASFGTGELLLVAVGAVLLTGVAFLAGMAVFAMV
                     RLFAFTFLAEPRSAGARLAAEPPAPLRSPVAVLALAGLALGVLAPWASPLLPIPLTRA
                     AWTVSAPPAPAAARAATAAAPVSALPLALAAALAGALVCAWLAGRLLAPGRRVRRVRT
                     WDCGQPIDATMEYTATGFSAPLRFFLRDIVTAEKHLVFSRVHPANPWITRGSMEFRKA
                     AGAVERIYLPIAGFIEGVGARLKLLQNGVIQFYVALMLVTLMLTLWVAL"
     gene            complement(3404..3940)
                     /locus_tag="PLT35_08290"
     CDS             complement(3404..3940)
                     /locus_tag="PLT35_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29265.1"
                     /translation="MPLARLLRSSALRPRLVGTDRSPRHLGGDRPAPEDAAALRAIVS
                     GLYGGRSLRIRAVDAGSTNAEELELTALGNAYYDIERLGLSFVASPRHADVLMVTGPV
                     VRNLAGALRATYDAAPSPCIVVAVGDGACTGSIWKGSYAVVGAVDEVIPVHIRIPGDP
                     PSPDDIIRGLAAGLRGAR"
     gene            4061..>4704
                     /locus_tag="PLT35_08295"
     CDS             4061..>4704
                     /locus_tag="PLT35_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG29266.1"
                     /translation="MKPSPPAPDSRTRERLLDAAERLFASRGFPGASVREITEAAGAN
                     LGAVNYHFRSKAGLYAEVFARRAALLRAPVLAAARDAAAHARRDPARAFRALGLAFLA
                     PHADRRASVRVFELLAREVVDRCLPRRHFAERFVVPALDAIASVVREVRPDLPDHVGQ
                     ACAHAFFVQLMAVVKAAVTSGKSIDERLDEAVRFTVAGDVPIAVENWSGGSVAAR"
BASE COUNT          757 a         1697 c         1774 g          476 t
ORIGIN      
        1 ggaagcccag caggatgagc gccagggcga cgaagagcag gccggccagc ggcagctctc
       61 cgccctccgc gcggccggac gccgccgaca gcggcagccg ccgcgccagc agccaggcgc
      121 cggccacgct cgcggcggcc agcgtgtagg acagcaccgg ccgcgtcatc gacgtgagcc
      181 gcatcgcgtc gccgagcttc cgcgccgtgc ggagcaggcc gaggggaatg agcaccacct
      241 tcaggaccag cgtggccgcc gcgcccgcgt actcggccgg gttgcccgac accgcggcac
      301 tgaccaggat gacgccggcc aggcaggccg acgaggcagc atagtggcgc aggaacgtcg
      361 tcaggcgcgt gcggccgatc gcgaagaacg ccgccagcag catgcagccc gtgaagacgc
      421 tcgagagcag cgccgggtcc ggtctcactt gaaccccgcc atcagcccga tgccaaggct
      481 cgtcagcccg aggaacagcg cgccgaagaa gaactccggc agcctgtaca accggagctt
      541 ggccatgccc gactcgacga gcgcgaggcc gcccatcgtc acggcgagct tggcgattgc
      601 cgcgggcacg gcggcggcgg cggccagcgg gccggcgccc gccctcagga ggccggccgg
      661 caccagcagg ttggcgagca gcagcgcgaa ggtggccagc ttgacggcgc tcgccagctc
      721 caggatcgcc aggtgcggac cggagtactc gagcagcatc gcctcgtgca ccatggtcaa
      781 ttcgaggtgg gtggccggat tgtccaccgg gtagcgcgcg ttctcgccga gcgccaccag
      841 cgccagcccg ccgacggccg ggaggagcca ggggcgcagg agcaggcctc tgccggcgac
      901 gtccatcatg gcgtcgacgg ccggggatcc cgacgcgatt gcgagcgcgg ccagcgacac
      961 gacgagcgcc ggctcgagga aggcggagat cgtcatctcg cggctcgcgc ccatcccgcc
     1021 gaacgcgctg ccggggtcga gcccgcccag caccaggaag acggagccga gcatccagat
     1081 cccggagacc gcgagcaggt gcgacagcgg cgccagcgcg ccgccgcgcg agacgagcgg
     1141 caggacgcac gcggcgagga gcgacgaggc gagcacggcc gtcggcgccg cgaggaagac
     1201 ccacgacgcg gactgcgaca cgacggtctc cttccggaag agcgtcgcga tcgtccagta
     1261 cgggagcagc gtcggcggcc cctggcggcc ctgcaagacc gccttgaagc ggcgcaccag
     1321 tcccggcgcc agcggcgcca gcgccacgac gatggcgccc tggacgaggg tgagcagcgc
     1381 cgggctcaca gggcgaccca gagcgtcagc atcagcgtga cgagcatcag cgccacgtag
     1441 aactggatga cgccgttctg cagcagcttc aggcgcgccc cgacgccttc gatgaagccc
     1501 gcgatgggca ggtagatccg ctccacggcc ccagcggcct tgcggaactc catcgatccg
     1561 cgcgtgatcc aggggttggc cgggtgcacg cgcgagaaca ccaggtgctt ctcggccgtg
     1621 acgatgtcgc gcaggaagaa ccggagcggc gccgagaagc ccgtcgcggt gtactccatc
     1681 gtcgcgtcga tgggctgacc gcagtcccag gtccggacgc ggcggacgcg ccggccaggc
     1741 gcgagcagcc gcccggcgag ccaggcgcac acgagggcgc cggcgagggc cgccgccagg
     1801 gccaggggca gcgcggacac gggggcggcg gccgtcgcgg cacgagccgc cgctggggcc
     1861 ggcggcgccg agaccgtcca ggcggcccgg gtcaggggga tcggcagcag cggcgacgcc
     1921 cacggcgcca gcacgcccag cgccagcccc gcgagcgcaa gcacggccac gggcgaacgc
     1981 agaggcgcag gcggctcggc cgccaagcgc gcgccggccg atcgcggttc ggcgaggaac
     2041 gtgaacgcga acaggcgcac catcgcgaag accgccatgc ccgcgaggaa cgcgacgccc
     2101 gtcagcagca cggcgccgac ggccacgagc aggagttctc cggtgccgaa cgacgccagc
     2161 agcgactcca cgagcagcca ctccgccgcg aaggcgccaa acggcggcag cggggcggcg
     2221 gcgagggcca gggcgagcat cgcggccgac aagcgcggca tccggttcgc gaggccgccg
     2281 agatcctcga tcttcgccgt gtgcgcgccg tggaggaccg cgccggctcc gaggaacagc
     2341 cccgccttga acagcgcgtg cgcgaacacg tggaggaaca tcgcgccgag gatggccgag
     2401 gccagccgtg ggtggccgtg cgcgccggcc agcgcggaga agccgaacat ggcgaagacg
     2461 agcccgaggt tctcgacgct gctccaggcg aggatgcgct tgatgtcgcg ggcgatgttc
     2521 gcggagatca ccgcgacgac cgctccgccg aggccgaggg cggccagcgc caggctccag
     2581 ccgtccggga gcgccggcca gcagatcgag gtgaaccgca gcaggccgta gacggcgatc
     2641 ttgagcatca cgcccgacat cagcgcggaa acctggctcg gcgcacgggg gtgcgcctcc
     2701 ggcagccatg cgtggaacgg cgcgagcccg gccttcgagg tgaaggcgaa cgcgaccagg
     2761 cccagggcca gcgtccggct tgccggcggg agcgcgggca ggcccgtgcc gagcgcggcc
     2821 agggtgactc gcgcggatcc gccgccgagc acgccgatgc cgacgagcag ggcaagcgcc
     2881 ccggcctggg cgacggcgag gtagagcagc gccgccttcc gcgagtcggc cgtgccgtcg
     2941 gccatcacga gaaagaacga cgacaccgac atcgcctccc agaacagcag gaagctggcc
     3001 gcgccggaag agagcaacac cccgtgcatg ccgaggcaga agacggccgt gagcggcacc
     3061 accaggcggg gcgggtactt cgccgcttcg tcttcgccgt accgccaccc gaaccaggcg
     3121 gccatgagcg acacgccgtg gagcagcatc aggaagaacg cggagagccg atcgacgacg
     3181 aacggcgcgc cgaagaggat cggcggcgcg gcgagcttga gcggcggcag ggccgcgagc
     3241 tggtgcgcca gcgaggccag gaggccggag gccgaaccgg cggcgagcag cgcgtgcgcg
     3301 agctgcctca cccgcggtcc gcgcgccgcc aggccggcga tggcggcgaa cacccacagc
     3361 agcgcggccc cggggaggcc cgcggcgaca gcctggccgg ccgtcaccgc gcccccctca
     3421 ggccggcagc caacccacgg atgatgtcgt ccggagaggg cgggtcgccc ggaatgcgga
     3481 tgtgcaccgg aatgacctcg tcgaccgcgc ccacgacggc gtacgagccc ttccaaatgc
     3541 tcccggtgca tgcgccgtcg ccgacggcca cgacgatgca ggggcttggg gcggcgtcgt
     3601 aggtggcgcg cagggcgccg gcgaggttgc ggacgaccgg cccggtgacc atcagcacgt
     3661 cggcatggcg cggggaggcg acgaaggaca ggccgagacg ctcgatgtcg tagtaggcgt
     3721 tgccgagggc ggtcagctcg agctcctcgg cgttcgtcga tccggcgtcg acggccctga
     3781 tgcgcagcga gcgtccgccg tagaggccgg acacgatggc gcgcagcgcg gcggcgtcct
     3841 ccggcgcggg ccggtctccg ccgaggtgcc gcggcgagcg gtcggtcccg acgaggcggg
     3901 gcctcagggc ggacgagcgg agcaggcggg cgagcggcat acgggtgcgg gcggatccga
     3961 caggcggata ctatagcatg cggccggagc gggcccggcg cggggcttga cacgccgcgt
     4021 cgccgaatgc taggttcaaa caattgtttg atacggttgt atgaagccct ctccgcccgc
     4081 tcccgactcc cgcacccgtg agcgcctgct cgacgccgcc gagcgcctct tcgcctcgcg
     4141 cggcttcccg ggggcgtccg tccgggagat caccgaggcg gctggcgcga acctcggcgc
     4201 cgtcaactac cacttccggt ccaaggccgg cctctacgcc gaggtcttcg cgcgccgggc
     4261 cgcgctcctc cgggcgcccg tcctcgcggc ggcgcgcgac gccgccgccc acgcgcggcg
     4321 cgaccccgcc cgcgcgtttc gcgccctcgg ccttgcgttc ctcgcgccgc acgccgaccg
     4381 ccgggcgtcg gtgcgcgtgt tcgagctcct cgcgcgcgaa gtcgtcgacc gctgcctccc
     4441 gcgccgccac ttcgcggagc ggttcgtggt gccggcgctc gacgccatcg cgagcgtcgt
     4501 gcgggaggtg cggcccgacc tgcccgacca cgtcggccag gcgtgcgccc acgcgttctt
     4561 cgtgcagctg atggccgtcg tgaaggcggc cgtcacctcc gggaagtcca tcgacgagcg
     4621 gctcgacgaa gccgtccgct tcaccgtggc gggcgatgtc ccgatagctg ttgaaaattg
     4681 gagcggcggc tccgtcgctg ctcg
//
LOCUS       DAOSIF010000223         4668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16325_length_4668_cov_4.809452, whole genome shotgun sequence.
ACCESSION   DAOSIF010000223 DAOSIF010000000
VERSION     DAOSIF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16325_length_4668_cov_4.809452"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLT35_08300"
     CDS             <1..204
                     /locus_tag="PLT35_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29267.1"
                     /translation="RQTAVYAFQLCEFINPILPTSAVTMGVLGAARIPWERWARWFLP
                     LMLILVALSCLLLVPPVLLFEWN"
     gene            complement(220..849)
                     /locus_tag="PLT35_08305"
     CDS             complement(220..849)
                     /locus_tag="PLT35_08305"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOG29268.1"
                     /translation="MSSTFRWRNFVTCALALSSLVVAVSGVVLFLRPEGSLARWVGWS
                     ALGADKRQWEALHLASVSLFLAAALAHAWYNWNAIAAAVSARRPRAPRRRLRLRPEFI
                     AALAAVGFVTAGSLADWQPLTALNALRSAIKDGRLAVSVPPPAADADRMSVRELCAKI
                     PLDPAEAMANARARGLAIADPSKTLGEAAAELGVSPEALYQAMCRPVRP"
     gene            complement(1053..2429)
                     /locus_tag="PLT35_08310"
     CDS             complement(1053..2429)
                     /locus_tag="PLT35_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG29269.1"
                     /translation="MSDSTANPQPPASKPPELARVLGLFSIVSIVIGTMIGSGIFINP
                     ANVARDVGTPGLMLLVWVAGGLLSFFGALAIGELAAAYPQAGGIYVYLREAYGPALAF
                     LFGWTLFLIIESGTIATLAVGFSTKYLPYFVTLSPAQGKVVAVSLIALLAAVNVLGVR
                     KGAFLMNFLTTIKFVGLIGVCAVVFIFASGSAANFTDGGAAGAGQGGVLGNFGVALVA
                     ALWAYKGWETSTYSAGELRNPERTLPLGLFIGTLCVIALYILANLAYLYVFPAARMAG
                     SNRIAADVMEAAVGPIGASIIAVITLLSMAGTANGHLLTSPRVFYAMARDGLFFSGVA
                     KVHPAYRTPHVSIVLVAAWAALLSLSGTFEQLFSFVIFGFWIFMGLTVLGVVILRRRQ
                     PDLPRPYKTWGYPVTPALFILSSVFLTANSLLRAFWNSFAGLAIIALGIPVYLVWKRR
                     AGRAGVRD"
     gene            2577..3443
                     /locus_tag="PLT35_08315"
     CDS             2577..3443
                     /locus_tag="PLT35_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOG29270.1"
                     /translation="MKRLPLACLVLLAAGAASEQVPAAVRVGVCVGAGEIEAAKAAGF
                     DYVEINASRVAALTEEEFRQLADRVARIGVPVEAANIFIPGAIKLTGPEVDPARQAAH
                     LELTLGRLEKLGVKVVVLGSGGARRVPDGFSADEARTQLVDFCRRLAPIARAHGITIA
                     VEPLRRQETNIVNTAREGLAFVKAVDRPEIQLLVDYYHMAEEGESADVLLEAGKAVVH
                     THIANPRGRVYPLDPAESNYAPFFENLCAIGYTGRLSIEASTKDFAAEAPAALAMLRK
                     GLACGPGPKAAR"
     gene            3540..>4668
                     /locus_tag="PLT35_08320"
     CDS             3540..>4668
                     /locus_tag="PLT35_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29271.1"
                     /translation="MSKVTRRTFMQAAGAGVAGAAIASSSSSYASVIGANDRVRVGLV
                     GYSDRTRSSLVPAFLRSADALNFEIVAVSDIWSLRREAGAAHIEKLTGKRPAATRNNE
                     ELYARKDVDAVIVGTADFQHAQHGVEAVRSGRDAYVEKPLANTMEDARAIRQAVKETG
                     RIVQIGTQRRSEPVYQRACEYIKSGRFGDIVMVEMTWNVNQPGRWRRPDVVPTLREQD
                     TDWGRYLLNRPFEPFDPRKYLEFRLFWPYSSGIPDQWLVHQIDTVHWFTGLPRPRSVV
                     ANGGIYLWKDGRRNWDTMTAVFDYGPLDDPSKGFQVVYSSRMTNSAGDVKELYYSNGG
                     TLDLDKNRVTPGGGLQAREAAAMKMPPNLLAEEALVSAAGPV"
BASE COUNT          764 a         1610 c         1703 g          591 t
ORIGIN      
        1 cgccagacgg ccgtctacgc gttccagctc tgcgagttca tcaatccgat ccttcccacg
       61 agcgcggtga cgatgggcgt gctgggcgcg gcgaggatcc cgtgggaacg gtgggcgcgc
      121 tggttcctcc cgctgatgct gatcctggtg gcgctgagct gcctgctgct cgttccgccc
      181 gtgctgctct tcgagtggaa ctgagggcga ggcggacggc tacggcctga ccggccggca
      241 catggcctgg tagagcgcct ccggcgacac gcccagttcg gcggccgctt cgccgagcgt
      301 tttcgacggg tcggcgatgg ccaggccgcg cgcccgcgca ttggccatcg cctcggccgg
      361 atccagcgga atcttcgcgc acagctctct cacgctcatc cggtcggcgt cggcggcggg
      421 cggcgggacc gagacggcga gccggccgtc cttgatggcg gagcgcagag cgttcagcgc
      481 ggtgagaggc tgccagtccg ccagcgaccc ggccgtgacg aacccgacgg cggcgagggc
      541 cgcaatgaac tccgggcgca aacgaagccg ccggcgcggc gcccggggcc ggcgcgccga
      601 cacggccgcc gcgatcgcgt tccagttgta ccaggcgtgc gccagcgccg cggcgaggaa
      661 cagcgagacc gacgcgaggt gcagcgcctc ccactgccgc ttgtcggcgc cgagcgccga
      721 ccacccgacc caccgggcga ggctgccctc gggccggagg aacagcacga cgcccgacac
      781 ggcgacgacc agcgacgaga gcgccagcgc gcaggtgacg aagtttcgcc accggaacgt
      841 cgatgacatg agcagcctcg agcaggggac gacgggcggg ctcacccggc gccctcgatt
      901 ctaccgtgtc cgcgccggga cggccgcgca ccctccgcga cggcgggtcc gccgccgccg
      961 gccgggcagc ccgcggccgg cgcccgcgcg cgccgcgcct gcaagcgcga gcctgaacgc
     1021 gccggggccg caatgggccc gccgtcgcgc cgtcagtcgc gaacgcccgc cctcccggcc
     1081 cggcgcttcc agacgagata gacgggaatg cccagcgcga tgatcgccag gccggcgaac
     1141 gaattccaga acgcgcgaag gagcgagttc gccgtgagaa agaccgacga caggatgaag
     1201 agcgccggcg tgaccgggta gccccacgtc ttgtacgggc gcggcaggtc cggttgcctc
     1261 cggcgcagga tcaccacgcc caggacggtc agtcccatga agatccagaa gccgaagatg
     1321 acgaacgaga agagctgctc gaacgtgccg ctcaggctga gcagcgcggc ccacgccgcc
     1381 acgaggacga tcgacacgtg cggcgtgcgg taggcggggt gcaccttcgc gacgcccgaa
     1441 aagaacaggc cgtcccgggc catcgcgtag aacacgcgcg ggctcgtgag gaggtggccg
     1501 ttcgccgtcc ccgccatcga gagcagggtg atgacggcga tgatcgacgc cccgatcggg
     1561 ccgacggcgg cctccatcac gtcggcggcg atgcggttcg atccggccat gcgcgccgcc
     1621 gggaacacgt agaggtaggc gaggttcgcg agaatgtaca gcgcgatgac gcagagcgtg
     1681 ccgatgaaga ggcccagggg aagcgtgcgc tccgggttcc gcagctcgcc ggcgctgtac
     1741 gtgctcgtct cccacccctt gtaggcccag agcgccgcga cgagcgccac gccgaagttg
     1801 ccgagcacgc cgccctggcc tgcgcccgca gcgccgccgt cagtgaagtt ggccgccgag
     1861 cccgacgcga agatgaacac gacggcgcag acgccaatca ggccgacgaa cttgatcgtc
     1921 gtcaggaagt tcatcaggaa cgcgcccttc ctgacgccga gcacgttcac ggcggcgagg
     1981 agcgcgatga gcgagacggc cacgaccttg ccctgcgcgg gcgagagcgt gacgaagtaa
     2041 ggcaggtact tggtcgagaa cccgaccgcg agcgtggcga tggtgcccga ctcgatgatc
     2101 aggaacagcg tccacccgaa gaggaacgcc agcgccgggc cgtaggcctc gcgcaggtag
     2161 acgtagatgc ccccggcctg cgggtaggcg gcggcgagct ctccgatggc gagggccccg
     2221 aaaaagctca gcaggccccc ggccacccag acgagcagca tcaggcccgg cgtgccgacg
     2281 tcgcgggcca cgttcgccgg gttgatgaag atgcccgatc cgatcatcgt cccgatgacg
     2341 atgctgacga tgctgaagag gccgagaacg cgcgcgagct cgggcggctt ggaggcgggc
     2401 ggctgcgggt tcgccgtgga gtcgctcatc ggatgtccct gacgtcgtgg aagccggacg
     2461 ggtgcccccg gagtatatgg aaacggcgcg ggcgcggcaa acgcgcgccg ggctgcctcg
     2521 cgccggcggc cgcgcggccg gcgcccgcga ggtgcgaatc ggttatgctt cagcccgtga
     2581 agcgcctgcc gctcgcctgc ctcgtcctgc tcgccgccgg cgccgcctcc gagcaggtgc
     2641 ccgcagccgt tcgcgtcggc gtgtgcgtcg gcgccggcga gatcgaggcc gcgaaggcgg
     2701 ccggcttcga ctacgtcgag atcaacgcct cgagggtcgc cgcgctgacc gaggaggagt
     2761 tccggcaact cgcggaccgc gtcgcccgca tcggcgtccc ggtcgaggcg gccaacatct
     2821 tcatccccgg cgccatcaag ctcactggac ccgaggtcga tccggcccgc caggcggccc
     2881 acctcgagct gacgctgggc cgcctggaga agctcggcgt gaaggtggtg gtgctcggca
     2941 gcggaggcgc gcgccgggtt cccgacggct tttcggcgga cgaggcgcgg acgcagctcg
     3001 tcgacttctg ccgacgcctg gcgccgatcg cccgcgccca cggcatcacg attgccgtcg
     3061 agccgctgcg ccgccaggag acgaacatcg tcaacaccgc ccgcgagggc ctggcgtttg
     3121 tgaaggcggt ggaccggccc gagatccagc tgctggtgga ctactaccac atggccgagg
     3181 aaggcgagag cgcggacgtc cttctggagg ccggcaaggc cgtcgtccac acgcacatcg
     3241 cgaatccaag gggacgcgtg tacccgctgg atcccgccga gtcgaactat gcccccttct
     3301 tcgagaacct ctgcgcgatc ggttacaccg ggcggctcag catcgaggcg tccacgaagg
     3361 acttcgccgc cgaggcgccg gcggcgctgg cgatgctgag aaagggcctg gcatgcgggc
     3421 cgggaccgaa ggccgcccgg tgacggcgcg caggtggacg acaggggacg cggcggccgc
     3481 gcggcctgcg agaggcgcga gacgatcgag gaggcgtcca tgacgtcggg agggagctca
     3541 tgtcgaaggt gactcggcgg acgttcatgc aggcggcggg cgcgggcgtg gcgggggcgg
     3601 cgatcgcctc cagctcgtcc agctacgcga gcgtcatcgg cgccaacgat cgcgtgcggg
     3661 tcggcctggt cggttactcc gaccggacga ggtcgtcgct cgttcccgcg ttcctcaggt
     3721 cggccgacgc gctcaacttc gagatcgtcg cggtgtccga catctggagc ctgcggcgcg
     3781 aggcgggagc cgcgcacatc gagaagctga ccggcaagcg gccggccgcg acgcgcaaca
     3841 acgaggagct gtacgccagg aaggacgtgg acgccgtcat cgtcgggacg gcggacttcc
     3901 agcacgcgca gcacggcgtc gaggccgtcc gatcggggcg cgacgcctac gtcgagaagc
     3961 cgctggcgaa cacgatggag gacgcccggg cgatccgcca ggcggtgaag gagacgggca
     4021 ggatcgtcca gatcggcacg cagcgccgca gcgagccggt gtaccagcgc gcctgcgagt
     4081 acatcaagtc gggcaggttc ggcgacatcg tgatggtgga gatgacctgg aacgtgaacc
     4141 agcccggccg ctggcggcgt ccggacgtcg tcccgacgct ccgggagcag gacaccgact
     4201 gggggcggta cctcctcaac cggcccttcg agccgttcga tccgcgcaag tacctcgagt
     4261 tcaggctgtt ctggccgtac tcgtccggca ttcccgacca gtggctggtc caccagatcg
     4321 acaccgtcca ctggttcacc ggcctgccgc gcccgcgcag cgtcgtcgcc aacggcggca
     4381 tctacctgtg gaaggacggc cggcgcaact gggacacgat gacggcggtc ttcgactacg
     4441 gcccgctcga cgacccgtcg aagggcttcc aggtcgtcta ctcgtcgcgc atgacgaact
     4501 cggccggcga cgtgaaggag ctctactact cgaacggcgg aaccctcgac ctcgacaaga
     4561 accgggtgac gcccggcggc ggcctgcagg cgcgcgaggc ggcggccatg aagatgcccc
     4621 ccaatctcct cgccgaggag gctctggtga gcgcggcggg gccggtga
//
LOCUS       DAOSIF010000224         4654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16383_length_4654_cov_3.771907, whole genome shotgun sequence.
ACCESSION   DAOSIF010000224 DAOSIF010000000
VERSION     DAOSIF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16383_length_4654_cov_3.771907"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..402)
                     /locus_tag="PLT35_08325"
     CDS             complement(28..402)
                     /locus_tag="PLT35_08325"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG29272.1"
                     /translation="MTLQETLRNILKHEGVVALASLGEDGPHLVDTWNSYVEVPSDCR
                     MLVPVGGMHRTERHIAVNPDVLITLGTRQEQGRHGPGIGFLIKGRAELAQAGPEFDRM
                     KARFPWMRAVLSIHIVSATQTL"
     gene            complement(512..1369)
                     /locus_tag="PLT35_08330"
     CDS             complement(512..1369)
                     /locus_tag="PLT35_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG29273.1"
                     /translation="MFNSRAMMVGLFAVFVFAAPARAQKDAAGCRDHPSLTRMPQYAL
                     VECRASDFDAYEFYDPLTQGKTKVTAEGRKIWLSYEVKAEYADKSPSQLQIVRNYETA
                     IQKIGGKTYSWTNPIGGELHARYAAGGLEIFARVYINGRGKGYTVTFIEKEAMRQEVT
                     ADAKTMAADIGATGRAVLYGIFFDVDKAEVKPESEPALTEIARLLKQDPALKLYVVGH
                     TDGTGVLEHNMRLSQMRAEAVAKELASKHGVAADRLKPHGVGPLAPVATNDTEEGKAR
                     NRRVELVKR"
     gene            complement(1408..2898)
                     /locus_tag="PLT35_08335"
     CDS             complement(1408..2898)
                     /locus_tag="PLT35_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29274.1"
                     /translation="MAARSRARWPAGALWFAGIACVSGLLLTGVRPGDAGQAASGCEG
                     CDRRAYVGRAEAGATGVALGESLVSRLAEVLTSPCFHLQRPIAGAPESEAARFGAKAE
                     PPEYVFEAEYEEGLNARADDGSAVMSRLTLTLFYDGEPRERVASWTVTSPINSFTACT
                     NRMFQNKDAAMKGVRPIEALLEDFERRPASCQIHPGKTDLACGETMDLIIAVLRDGNG
                     RETRPFNRIVVEAEKGEVLGGEPVEGRPRSRAFLVDDGIIELRYRAPGGETGAVEDVI
                     SVFSSCAVARPDRLPLSKTSPFRKIGESRLTVHCGAAPGTVVYRRTVSWDDTAANQAG
                     STTYKGSLSEEVTVRVGLQYTNTYKDNDYYRAATTSATYSFSYANDVFINGRKADVLS
                     ERVEDSGAFGPEGSASIELVVGRKSGAYTLQIGLRSREKQGRGSWFIGVNTLPDLLKG
                     AVDGRSARGSYAAPAGKGGRWPDHRDNQGNDPGTSFSWNLELPDRK"
     gene            complement(2919..>4654)
                     /locus_tag="PLT35_08340"
     CDS             complement(2919..>4654)
                     /locus_tag="PLT35_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29275.1"
                     /translation="SDLRNALERRASPSELRRLQRALGKAGPAGGLNGYGMALLLAGS
                     PGDALYVLSVAAAGFPDDFLAFNNLGAVLNNLGQYRLARPLLRYADALSPDNPMVLSN
                     LGAAELGEGRSAEAEKLFSRAVKIAPNHPQANYVLGRLALRRNDLAAARRHLEVSLDG
                     SYTAAAAKALKDVDRALRRSGSAKAEPPGALPPVPLLAAEAGKDALVRLPMVQAGNSA
                     ECVSARKWLYAEREKYAAMATATLAELRPYLTAGPGSRPPGESAAASAQPSGARVLRL
                     TPDKAKRGQKGAELWLERLSGSRERFLRDIHARSMEALRIHGELDKAWLAEEEQCLKL
                     EPNARGRCLDEVKRRSCARHAQTFDGEALAVSEAYAQFAPAWEAAALAYHRTVNYWAS
                     FIPDPAEAARARIGARGAILQNIDQIVGDMHLMYAVLSPDESCLTTPPPAQSIEGELR
                     LEDFKVPCTFPGLGLDLAVVSFHVDCTTVTVGGDIGVAAVELEWNFVEKTGTLFIGAE
                     TGFEAGKLVSAEASARAGLVLSFDGDSMTDIGLEARASAGIRLGQVLTADRADAEAGV
                     RIGLNSTPTFF"
BASE COUNT          699 a         1579 c         1577 g          799 t
ORIGIN      
        1 catgagcgcg gggccgtggg ccatgggcta cagcgtctgg gtggcagaca cgatgtggat
       61 ggacagcacg gcccgcatcc acgggaagcg tgccttcatg cggtcgaact ccgggcccgc
      121 ctgcgcgagc tcggccctcc ccttgatcag gaacccgatg ccggggccgt gccgaccctg
      181 ctcctgtcgg gtcccgaggg tgatcagcac gtcggggttc accgcgatgt ggcgttcggt
      241 tcgatgcatc ccgccgacag gaacgagcat gcggcagtca gacgggacct cgacgtagct
      301 gttccaggtg tccacaaggt gcgggccatc ctcgcccagg ctcgccaggg cgaccacgcc
      361 ctcgtgtttc agtatgttcc tcagcgtctc ctgaagcgtc atcgctgcct ctcgaacagg
      421 acacacgaac catcatacaa gccccgctgc cgctgccgcc acggcaagcg agcgggcact
      481 ggtccgaccc gcacccccgg acgaatccgg gctagcgctt gaccagctcg acgcggcggt
      541 tcctggcctt gccctcctcg gtgtcgttgg tcgcgaccgg cgccagcggg ccgacgccgt
      601 ggggcttgag ccggtcggcg gcgactccgt gcttcgaggc gagctccttc gccacggcct
      661 ccgcccgcat ctgcgagagc ctcatgttgt gctccaggac gccggtgccg tcggtgtggc
      721 cgacgacgta gagcttcagg gccgggtcct gcttcaggag cctggcgatc tcggtcaacg
      781 ccggctcgga ctcgggcttg acctcggcct tgtcgacatc gaagaagatg ccgtagagga
      841 ccgccctgcc cgtcgcaccg atatcagcag ccatcgtctt ggcgtcggcc gtaacctcct
      901 gccgcatggc ttccttctcg atgaacgtga ccgtgtagcc tttccccctc ccgttgatgt
      961 aaacgcgggc gaagatctcc aggccgcccg cggcgtacct ggcatggagc tcgccgccga
     1021 tggggttggt ccaggagtag gtctttccgc cgatcttctg gatggccgtt tcgtagttgc
     1081 ggacgatctg cagctgcgac ggggacttgt cggcgtactc ggccttgacc tcgtagctga
     1141 gccagatctt ccggccctcg gccgtaacct tggtcttgcc ttgggtcagc ggatcgtaga
     1201 actcgtacgc atcgaagtcg ctggccctgc attcgacgag ggcgtactgg ggcattctcg
     1261 tcaacgaagg gtgatcccgg cagccggccg cgtccttctg ggcccgggcg ggggctgcga
     1321 agacaaagac cgcgaacagt ccgaccatca tggctcgtga gttgaacatg atcctcggcc
     1381 tcctcttgat gtcgggcgcg gcggcgccta tttccgatcc gggagctcca ggttccagct
     1441 gaagctcgtc ccgggatcgt ttccctggtt gtcgcgatga tcgggccagc ggccgccctt
     1501 cccggcgggc gcggcgtagg agcctcgcgc gctccggcca tcgaccgctc ccttcagcag
     1561 gtcggggagc gtattgacgc cgatgaacca tgagccgcgg ccctgttttt cgcggctgcg
     1621 caggccgatc tggagtgtgt aggcgccgga cttccggccg acgactaact cgatagacgc
     1681 gctgccctcc ggtccgaaag cacctgagtc ctcgaccctt tcgctcagga cgtcggcctt
     1741 gcggccgttg atgaaaacgt cgttcgcgta gctgaaggaa tatgtcgccg acgtcgtcgc
     1801 ggctcgatag tagtcgttgt ccttgtaagt gttcgtgtac tggagcccga cccggacggt
     1861 gacctcctcg gacaggcttc ctttgtaggt cgtggacccc gcctggttgg ccgcggtgtc
     1921 gtcccaggac accgtccggc ggtacacgac ggtcccgggc gcggccccgc agtggaccgt
     1981 caggcggctc tcgccgatct tccggaaggg ggaagtcttc gagaggggca gacgatccgg
     2041 ccgggccacg gcacaggagc tgaagaccga aatgacgtct tcgacggcgc ccgtttcgcc
     2101 gccgggcgcc cgatagcgca actcgatgat cccgtcgtcg acgaggaagg cccggctcct
     2161 cggacgccct tcgacgggct cgcccccgag gacttcgccc ttctccgcct cgacgacgat
     2221 ccgattgaag ggcctggtct cccgcccgtt cccgtctcga agcacggcga tgatgaggtc
     2281 catcgtctcg ccgcaggcca ggtccgtctt gcccggatgg atctggcagc tcgccgggcg
     2341 cctctcgaag tcctcgagga gcgcctcgat cggccggacg cccttcatcg ccgcgtcctt
     2401 gttctggaac atccgattgg tgcaggccgt gaacgagttg atcgggctcg tgaccgtcca
     2461 gctggccacc cgctcgcggg gctccccgtc gtagaacagc gtgagggtca gacgagacat
     2521 cacggccgag ccgtcgtcgg cgcgggcgtt caacccctcc tcgtactcgg cttcgaacac
     2581 gtattccggc ggctcggcct tcgccccgaa ccgggccgct tccgactcgg gtgcgccggc
     2641 gatgggccgc tggagatgga agcaggggga ggtcaggacc tcggccaggc gggagacgag
     2701 agactccccg agggcgacgc cggtggcccc ggcctcggct cgtccgacgt aggcccgccg
     2761 gtcacagccc tcgcagccgg acgcggcctg ccccgcgtcc ccgggacgga cgccggtgag
     2821 gagcaggccc gaaacgcagg cgatacccgc gaaccacaag gctccggcgg gccatcgagc
     2881 ccttgatcgc gcggccatcc gtccctcctt tccctctctc agaagaacgt cggcgttgaa
     2941 ttgaggccga tccggacgcc ggcctcggcg tcggcccgat cggccgtgag aacctggccg
     3001 agccggatcc cggccgaggc tctggcctcg agcccgatgt cggtcatcga atcgccgtcg
     3061 aagctgagga cgagcccggc cctggcggag gcctcggccg aaacgagttt tccggcctcg
     3121 aaacccgtct cggcgccgat gaagagcgtc cccgtcttct cgacgaagtt ccactccagt
     3181 tccacggcgg cgacgccgat gtccccgccg acggtcacgg tcgtgcagtc gacgtggaag
     3241 gacacgacgg cgaggtcgag cccgagcccg gggaatgtgc acggcacctt gaagtcctcg
     3301 agccggagtt cgccctcgat cgactgggcg ggcggcggcg tcgtgaggca gctttcgtcc
     3361 ggggacagca cggcgtacat aagatgcatg tctccgacga tttggtcgat attctgcagg
     3421 atggcgcctc gcgctccgat cctggcgcgg gccgcctccg cgggatcggg gatgaagctg
     3481 gcccagtaat ttactgtccg gtgataggcc agggccgcag cttcccacgc cggggcgaat
     3541 tgggcatagg cttctgagac ggcgagggct tcgccgtcga aggtctgggc atgccgggcg
     3601 caggagcggc gcttgacttc gtcgaggcaa cggccgcggg cattcggctc gagcttcagg
     3661 cactgttctt cctcggccag ccaggccttg tcgagctcgc cgtgtatcct taaagcctcc
     3721 atcgagcggg catggatgtc gcggaggaaa cgctcccgcg atccgctgag acgctcgagc
     3781 catagctcgg cgcccttctg cccccgcttc gccttgtcgg gcgtcagccg gaggacccgc
     3841 gccccggacg gctgcgccga ggcggccgcg ctttcaccgg gcggccgtga tcccgggccg
     3901 gccgtcaagt aaggccggag ctcggccaac gtggccgtgg ccatcgccgc gtacttctct
     3961 ctctccgcat acagccactt ccgggcggaa acgcactccg cgctgttgcc cgcctggacc
     4021 atcggcaggc ggaccaaggc atcctttccg gcctcggcgg ccagtaaggg gaccggcggc
     4081 agcgccccgg gaggctcggc tttggccgag ccgctccggc gcagggcgcg atccacgtcc
     4141 ttgagggctt tggccgcggc cgccgtatag gagccgtcga gagaaacctc gaggtgccgc
     4201 cgggccgccg cgagatcgtt ccgccggagg gccagccggc ccagcacgta attggcttgg
     4261 ggatggttcg gggcgatctt gacggcccta gagaagagct tctccgcttc ggcagagcgg
     4321 ccttcgccga gttcggccgc gcccagattg gacaagacca tcgggttgtc cggggagagc
     4381 gcatcggcgt atcgaaggag aggccgggcg agtcggtact ggccgaggtt attgaggacc
     4441 gcacctaggt tgttgaaggc caaaaagtcg tccggaaatc cggccgcggc gacggagagg
     4501 acatagaggg cgtcgcccgg agaacccgcg agcagcaggg ccatcccgta gccgttgagc
     4561 ccgccggccg gtccggcctt tcccagcgcc cgctgaagcc ggcggagctc cgacggggaa
     4621 gctcggcgtt caagcgcgtt ccggaggtcc gata
//
LOCUS       DAOSIF010000225         4647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16414_length_4647_cov_4.374347, whole genome shotgun sequence.
ACCESSION   DAOSIF010000225 DAOSIF010000000
VERSION     DAOSIF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16414_length_4647_cov_4.374347"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PLT35_08345"
     CDS             <1..1105
                     /locus_tag="PLT35_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29276.1"
                     /translation="RDTAAPWLRWLSQAADAARGAPSLFRGTPWLAFAEAGVWAVALG
                     AAWFAARLAERTWRLRPGSAALTLLGAFALAGGLSLTAAWRLEGVAGLAAAAGQMRAL
                     EHAAAGKSGCGVSLDPLVFSSPEAALGRLRLGEARGGLPPGALLQLPGLPAGRYRLWI
                     DRPAGSAAADAGLFVGTRAGPPVCAWRIEEAPAGAVSREFELPVGVSSLALRWIGEPQ
                     APVRAAWLQPAGRAGGNRLLHEMRASAAARYGRAVVFAIGRAFLEPEGLWTGGDTTAE
                     LVVQSASGEDHSDFTMGAGPVDTSVEVTAGPFAWRGTLAPGERRGLRLPTGAGGAVLV
                     RIRTTPGFRPASHDPSSGDVRLLGVRLDPPAQP"
     gene            complement(1199..2038)
                     /locus_tag="PLT35_08350"
     CDS             complement(1199..2038)
                     /locus_tag="PLT35_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29277.1"
                     /translation="MTRVPAARALLTAALAVLAVSVPAEAQYRPIPARAAGPDAGPFG
                     GWYRIEFAANTWNPVPEFTVSSESLGIKGTDIDVRADLDIPQERKTELRLTLRASKRN
                     KLRFHYLPLAYKGTTNLKADIIFNGIRYPANTSVDSSLEWKTYRVGYELDIISRTQGF
                     LGFVVEAKYTDASFTLTSPAGTEYARARAPIPALGAIARVHITRYGSVTGEFTGFKIP
                     ESIDEDYRGHYYDWDVYGTLNFTRNFGAQIGYRSLDIGYLARQDRGDAKLSGLYFGGV
                     VRF"
     gene            2209..4092
                     /locus_tag="PLT35_08355"
     CDS             2209..4092
                     /locus_tag="PLT35_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG29278.1"
                     /translation="MRPSPTRRLATYLLRYTRGFVLGLACTGLSTAFGLATPWVLKLA
                     VDDLAASATGAKFRTYALLVLGLALAGGAFRFLMRRIIVGVSRRLEFDLRNDLFARLL
                     TLPPAYYHAHRTGDLMSRATNDLNAVRMMAGPAIMYITETAIVFAVAIVLMAGLDARL
                     TAIALIPLPFVSIAARRFGAAIHRRFEGIQAQLSALSAVVQEALSGVRVVRAYRQEEA
                     EFARFADANTEYVRRNLGLIRVQSAFFPGLGLLLGLGGLLVLWLGSRDVIHGRITLGE
                     FVAFNAYMAMLTWPMIAFGWVTNMLQRGAASWQRMLEVLDAEPAVPDLEPDPARPAVG
                     PIRGGIEFRGLTFAFDGAPVLRDVSVAIEAGRTLAIVGPTGSGKSTLINLIPRLYDPP
                     RGTVFIDGTDVRDIPLAALRGAIGFVSQEPFLFSESLGENIGFGTRAGEAERSAASQP
                     PASTLDEPLAAAASVACLDKDVRDFPEGYATMVGERGITLSGGQKQRTALARAIAIDP
                     RILILDDALSAVDTYTEEEILSRLRGVMRERTSVIVSHRISTVRDADLVILLDGGRIV
                     ERGSHDELVALGGAYAGLYRKQLLEEELEGDRQEAGSERPGAGDGQQTNRRRAADPRS
                     PSG"
     gene            4097..>4647
                     /locus_tag="PLT35_08360"
     CDS             4097..>4647
                     /locus_tag="PLT35_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG29279.1"
                     /translation="MDGRDDEIIAGKAYDAVLMRRLLTYLRPYWRQVLLALVCIAAGS
                     MLQLAQPYLMKLAIDRYIADRDLAGLDRIALLFLGILGGAFAAEFVQAYVLQSTGQRI
                     MFDIRAEIYRHLQRLDIAFHDRNPVGRLMTRVTTDVDALNDLFASGVVSVFGDVFTLA
                     GIMIVLLTMDWRLALVAFSVLPA"
BASE COUNT          590 a         1606 c         1724 g          727 t
ORIGIN      
        1 acgcgacacc gccgcgccct ggctgcggtg gctgagccag gctgcggatg ccgcccgcgg
       61 cgcgccgagc ctgttccgcg gaacgccgtg gctggccttc gccgaggcgg gcgtctgggc
      121 cgtcgcgctc ggggcggcct ggttcgccgc ccgactcgcg gagcgcacgt ggcgcctgcg
      181 gccaggctcc gcggcgctca cgctgcttgg cgcgtttgca cttgccggcg gcctgtcgct
      241 cacggcggcc tggcgcctcg aaggcgtcgc cgggctggct gcggcagccg ggcagatgcg
      301 ggcgctggaa cacgccgccg ccgggaagtc cggatgcggc gtcagcctcg acccgctggt
      361 cttcagcagt cccgaggctg ctctgggccg tctgaggctg ggcgaggcgc gcggcgggct
      421 tccgcctggc gcgctgctgc agctacccgg gcttccggct gggagatacc ggctgtggat
      481 cgatcggccc gcgggttcgg cggcggccga cgcggggctc ttcgtcggaa cgcgagcggg
      541 gcctcccgtc tgcgcctggc gcatcgagga agcacccgcc ggggcggtga gccgcgagtt
      601 cgaattgcct gttggcgtgt cgtcgcttgc cctccgctgg atcggggagc cgcaggcccc
      661 cgtgcgggcc gcatggctgc agccggcggg ccgcgcgggc ggaaaccggc tgctgcacga
      721 gatgagggcg tcagccgcgg cccgctacgg ccgggctgtg gtgttcgcga tcggccgcgc
      781 cttcctcgag ccggagggcc tgtggaccgg gggagacacc acggccgaac tggtggtgca
      841 gtcagcgtct ggagaggacc attcggactt cacgatggga gcggggcccg tcgacacctc
      901 ggtggaggtg acggccgggc cgtttgcctg gcgggggacg ctcgcgccgg gcgagcggcg
      961 cgggctgcgg ctgccgacgg gcgccggggg cgcggtcctc gtgcgcatcc gcacgacgcc
     1021 gggctttcgc cccgcttcgc acgatccctc gagcggcgac gtgcgcctgc tcggggtccg
     1081 cctcgacccg cccgcgcagc cgtgacgcgc ttccggcgac gggacgaggc ggcggcctct
     1141 tcgccggcga ggcggcgact gccgccgcgc tcgagaggcc cgcgggggct cgcgtgcctc
     1201 agaagcggac gacgccgccg aaatacagcc cgctcagctt ggcgtcgcca cggtcctgcc
     1261 tggcgagata gccgatgtcg agcgaccggt agccgatctg ggcgccgaag ttcctcgtga
     1321 agttcagcgt tccgtacacg tcccagtcgt agtagtggcc gcggtagtcc tcgtcgatgc
     1381 tctcggggat cttgaacccg gtgaactcgc cggtgacgga gccgtaccgc gtgatgtgaa
     1441 cgcgggcgat cgcgccgagg gcgggaatcg gcgcgcgcgc ccgcgcgtac tcggtcccgg
     1501 ccgggctcgt gagcgtgaag ctcgcatcgg tgtacttcgc ctcgacgacg aagccgagga
     1561 agccctgcgt ccgggagatg atgtcgagct cgtagccgac ccggtaggtc ttccactcca
     1621 gactcgagtc cacgctggtg ttggccgggt acctgatgcc gttgaagatg atgtccgcct
     1681 tcaggttcgt ggtgcccttg taggcgaggg gcaggtagtg gaagcggagc ttgttccgct
     1741 tgctcgcccg gagcgtgagg cgaagctcgg tcttgcgctc ctgcgggatg tccaggtcgg
     1801 cgcggacgtc gatgtcggtg cccttgatgc cgagcgactc gctcgacacg gtgaactcgg
     1861 ggaccgggtt ccacgtgttg gcggcgaact cgatgcggta ccagccgccg aagggcccgg
     1921 catcggggcc ggcggctcgc gccgggatgg gcctgtattg ggcctcggcc ggaacggaca
     1981 cggcgagaac ggccaaggca gcggtcagca gggcgcgggc ggcgggtact cgggtcatgg
     2041 acggcgctcc tcggcgacac cacgggcgtc gcgcatgacc ctcttatcgg tgcgacgggt
     2101 gtgcgcgact gccccgcgct gcacgcaagt gcacggtacg gcacggaaac ggccggcccg
     2161 cgggcgcgct ggccggagcg gtgacggctc cggtatgctg aaggcctcgt gaggccttcc
     2221 ccgacccggc ggctcgcaac ctacctcctc cgctacacgc gcgggttcgt gctcggcctg
     2281 gcctgcaccg gcctgtccac cgcgttcggc ctcgcgacgc cgtgggtgct gaagctggcg
     2341 gtcgacgacc tggcggcgag cgcgacgggc gcgaaattcc ggacctacgc cctgctcgtc
     2401 ctcgggcttg cgctggccgg cggcgcgttc cgcttcctga tgcgccgcat catcgtcggc
     2461 gtctcgcggc gcctcgagtt cgacctccgc aacgacctct tcgcccggct gctgacgctc
     2521 ccccccgcgt actaccacgc gcaccgcacc ggcgacctca tgtcgcgggc gaccaacgac
     2581 ctgaacgccg tgcgcatgat ggcgggcccg gccatcatgt acatcaccga gacggccatc
     2641 gtcttcgccg tggcgatcgt gctgatggcg gggctcgacg cgcggctgac ggcgatcgcg
     2701 ctcatcccgc ttccgttcgt ctccatcgcg gcgcggcgct tcggcgcggc catccaccgg
     2761 cggttcgagg ggatccaggc gcagttgtcc gcgctgagcg ccgtggtgca ggaggcgctg
     2821 tcgggcgtcc gcgtcgtcag ggcctaccga caggaggagg ccgaattcgc gcggttcgcc
     2881 gacgcgaaca cggagtacgt gcggcgcaat ctgggattga tccgggtcca gagcgcgttc
     2941 tttcccggcc tcggcctgct cctcggcctg ggcgggctgc tcgtgctctg gctcggcagc
     3001 cgcgacgtca ttcacggccg catcacgctg ggcgagttcg tggcgttcaa cgcgtacatg
     3061 gccatgctca cctggccgat gatcgccttc ggctgggtga cgaacatgct ccagcgcggc
     3121 gcggcgtcgt ggcagcggat gctggaggtg ctcgacgccg agccggcggt ccccgacctg
     3181 gagcccgatc cggcccgtcc ggccgtgggc cccatccggg ggggcatcga gttccgcggg
     3241 ctcacgttcg ccttcgacgg cgcgccggtg ctgagggacg tctcggtggc catcgaggcc
     3301 gggcggacgc tggcgatcgt cggaccgacg ggatcgggaa agtcgacgct gatcaacctg
     3361 atcccgcggc tgtacgatcc gccgcgaggc acggtgttca tcgacggcac cgacgtgcgc
     3421 gacatcccgc tcgcggcgct gcgcggcgcg atcggcttcg tgtcgcagga gccgttcctc
     3481 ttctccgaga gcctcggcga gaacatcggc ttcggcacgc gggccggcga ggccgagcgc
     3541 tccgcggcga gccagccgcc cgcctcgacg ctggacgagc cgcttgcggc ggccgcgtcg
     3601 gtggcgtgcc tcgacaagga cgttcgcgac ttccccgagg gctacgccac gatggtcggg
     3661 gagcgcggca tcacgctctc cggcggccag aagcagcgca ccgccctggc gcgggcgatc
     3721 gccatcgatc cgcgcatcct catcctagac gacgcgctgt ccgcggtgga cacctacacg
     3781 gaggaggaga tcctgtcgcg cctgcggggg gtgatgcgcg agcggacgtc ggtgatcgtc
     3841 tcgcaccgca tctccacggt ccgcgacgcg gacctcgtca tcctcctcga cggcggccgg
     3901 atcgtggagc ggggctcgca cgatgaactc gtcgcgctcg gcggcgcgta cgccgggctc
     3961 taccggaagc agctgctgga agaggagctg gaaggggaca gacaggaagc aggaagcgag
     4021 aggccggggg caggagacgg acagcagacg aaccgacgcc gggctgccga tccccgatcc
     4081 ccgagcggct aaggcgatgg acggccgcga cgacgagatc atcgccggga aggcgtacga
     4141 cgccgtgctc atgcggcgtc tgctgacgta cctgcgcccg tactggcggc aggtgctgct
     4201 ggccctcgtg tgcatcgccg ccgggtcgat gctgcagctg gcccagccgt acctgatgaa
     4261 gctggccatc gaccggtaca tcgcggaccg cgacctggcc ggactggatc gcatcgcgct
     4321 cctgttcctg ggaatcctcg gcggcgcgtt cgcggccgag ttcgtgcagg cctacgtgct
     4381 gcagtcgacc gggcagcgca tcatgttcga catccgcgcg gagatctacc ggcatctgca
     4441 gcgcctcgac atcgcgttcc acgaccgcaa cccggtgggt cgcctgatga cccgcgtgac
     4501 gaccgacgtg gacgctctga acgacctgtt cgcctccggc gtcgtgtccg tgttcggcga
     4561 cgtcttcacg ctggccggga tcatgatcgt cctcctcacg atggactggc ggctcgcgct
     4621 ggtcgctttc tcggtgctcc cggccat
//
LOCUS       DAOSIF010000226         4641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16432_length_4641_cov_4.508286, whole genome shotgun sequence.
ACCESSION   DAOSIF010000226 DAOSIF010000000
VERSION     DAOSIF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16432_length_4641_cov_4.508286"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLT35_08365"
     CDS             <1..451
                     /locus_tag="PLT35_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29280.1"
                     /translation="SSDGAAAPIFNSYRDIARSGSRDLIGKNARIDATGVGLTFGTFM
                     LVFRSAWNSYLYYVFNSALFEYQSGTFLTSTINQLTVGNLNDMTVPVPPAEDAVSIAA
                     FLDRETARIDTLIAKVRDAIVRLRELRTTLISAAVTGKIDVREEAAA"
     gene            608..1768
                     /locus_tag="PLT35_08370"
     CDS             608..1768
                     /locus_tag="PLT35_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOG29281.1"
                     /translation="MKRTRTGTYETTAAGGETVRAFVPAPLPPVPPLVLDGAARDALD
                     AALLALGRLDGISSVLPDTHLLLYSYIRKEAVLSSQIEGTQSTLADLMLFELKEAPGV
                     PLDDVVEVSNYVRALEHGVKRLRGGFPLSNRLIREVHQILLSRGRGAGKSPGEFRRTQ
                     NWIGGTRLGNARFVPPPPHRVAAAMSDLEKFLHDPSAPPVLLRAALWHVQFETIHPFL
                     DGNGRVGRLMVTLLLCAAGVLREPMLYLSLFLKQHRARYYELLDSVRCEGDWESWVAF
                     FCEGVAAVSDGAVTSARRLLDLAGQDREQLARLGRRAGSAATVQTALLSTPVCTIPLL
                     AKRTGLTLPTIAKALDVLLDREMVSETTGKKRNRVYRYDRYLEILNEGTEPL"
     gene            1765..>4641
                     /locus_tag="PLT35_08375"
     CDS             1765..>4641
                     /locus_tag="PLT35_08375"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOG29282.1"
                     /translation="MSPEISERAFEQAIECGLLQHGPDACAGAASTVRETSPPYGESP
                     PGGYRRRRPEDYDRTLCLLPRDALDFVLATQPKEWKKLEQHHGTAVREQFIKRLAAEI
                     ARRGALDVLRNGLKDSGCKFRLAYFRPASGLNEETRRLHAANLFSVVRQLHYSTKNEK
                     SLDLVLFLNGIPIFTAELKNPLTGQDVEDAIRQYKTDRDPREPLLAYGRCLAHFAVDP
                     DLVYVTTRLAGTKTRFLPFNQGRFGGAGNPPVPPTRKGYATSYLWEETWARDSVLDLV
                     RQFIHEVEEEDDKGRKTGARYIIFPRYQQLDCVRRLVADARARGAGQRYLVQHSAGSG
                     KSFTIAWLAHQLSTLHDASDRRVFDSIVVITDRRVLDRQLQTTMRQFEQTLGVVENID
                     TTSRQLKEALESGKTIIVTTLQKFPVIAAEIGELPGRRFAVIVDEAHSSQSGESTKSL
                     KAVLASGSLEAAESEEAGAATPEEELENTVLAEMERRGHLPNLSTFAFTATPKPKTLE
                     LFGVKRADGKFGPFHLYSMRQAIEEGFILDVLASYTTYTAYWRLLKTIESDPRYDKKK
                     AEYLLKSFVELHPHAIAEKVKLCVEHFAAHAQGEIGGKAKAMIVTRSRLHAVRYKLAV
                     DKYVAERGYPFKALVAFSGTVQDGGQAYTEANMNGVPETQTAGTFERPDYRFLIVANK
                     FQTGFDQPLLHTMYVDKKLGGVNAVQTLSRLNRTHPEKKNTLVLDFANSADEIQRAFE
                     PYYETTLLSEATDPNLLYELQGRLAKFPVYTEADVNAFAKLYFDRGATQDQLYAVLAP
                     LVARWGQLPEDERLEFRGQLTDYVRLYAFLAQVLTFTDADLEKLYVFARHLRRLLPAD
                     RDVLPREVQQNIDMESYRIQQTGSGKIALERKPGVVDPAGSRTSHGAAPEELESLSRI
                     IAELNERFGLNPEVITSCDAVVRIPLFGLKNSLNVVSAFSIASYT"
BASE COUNT          824 a         1598 c         1500 g          719 t
ORIGIN      
        1 gagcagcgac ggagccgccg ctccaatttt caacagctat cgggacatcg cccggagcgg
       61 gagccgcgac cttatcggga agaacgccag aattgatgcg acaggcgtcg gtttgacgtt
      121 cggaaccttc atgctcgtct ttaggagcgc atggaactcg tacctatact acgtattcaa
      181 ttccgcgctc ttcgagtatc agtcgggaac ctttctgacg tccacgataa accagctgac
      241 cgtaggaaac cttaacgaca tgacggttcc ggttcctcca gcagaggacg cggtgtctat
      301 cgccgccttc ctcgaccgcg agaccgcccg catcgacacc ctcatcgcca aggtccgcga
      361 tgccatcgtc cgcctcaggg aactccgcac gacgctcatc tccgctgcgg taacggggaa
      421 gatcgacgtg cgggaggagg cggccgcatg acccgcgtgg ccatcgaggc ccggatgctc
      481 ggtcccgcgg ttcgtcccgg gcaggcagat cggtgcacgg attgacgttc gaggttaata
      541 aagcgaaaat gcatctgctt tagttacgta gggcgttact aaagtcgctt tcacaggagc
      601 cgcacgcgtg aaacggaccc ggaccgggac gtacgagaca acggccgccg gcggtgagac
      661 ggttcgggcg ttcgtgccgg ccccgctgcc gccagtgccg cccctcgttc tggacggcgc
      721 tgcccgcgat gcgctcgacg cggcgctgct cgccctgggc cggctcgacg gcatctcgag
      781 cgtgctgccg gatacgcacc tgctcctcta ctcgtacatc cgcaaggaag ccgtgctttc
      841 ctcgcagatc gagggtacgc aatccaccct cgccgacctg atgctcttcg agctgaagga
      901 agcgcccggc gtcccgctcg atgacgtcgt cgaggtgtcg aactacgtgc gcgcgctcga
      961 acacggggtg aaacggctgc gcggtggctt tccgctctcg aaccggctga tccgcgaggt
     1021 gcaccaaatc ctgctctcgc gcggccgggg tgcgggaaag agtccgggcg agttccgccg
     1081 gacccagaac tggatcggcg ggacgaggct cggcaacgcg cgtttcgtcc ctccgccgcc
     1141 gcatcgcgtg gcggccgcca tgagcgatct ggagaagttc cttcacgacc cttcggcgcc
     1201 gcctgtgctg ctcagggccg cactctggca cgtccagttc gaaaccatcc atcccttcct
     1261 cgacggcaac gggcgtgtcg ggcggctcat ggtcacgctg ctgctctgcg ccgcgggggt
     1321 gttgcgcgag ccgatgctgt atctgagcct ctttctcaag cagcaccgcg cccgctacta
     1381 cgagctgctc gactcggtgc gctgcgaagg cgactgggag tcctgggtcg cgtttttctg
     1441 cgagggcgtg gcagcggtgt cggacggcgc ggtaacgagc gcgcgccgcc tgctcgacct
     1501 cgccggacag gatcgcgagc aactggcccg cctcggccgc cgggcgggtt ccgcggcgac
     1561 ggtccagact gcgcttctca gcacgccggt ctgcacgatt ccgctgttgg cgaagcggac
     1621 ggggctcacg ttgccgacga ttgcgaaggc gctcgatgtg ctgctggaca gggagatggt
     1681 cagtgagacg accgggaaga agcggaatcg tgtgtaccgc tacgaccgct acctggagat
     1741 cctgaacgag gggacggagc cgctgtgagc cccgagattt ccgagcgcgc cttcgagcag
     1801 gccatcgagt gcgggctgct gcagcacggt cccgacgcct gcgccggcgc cgcgtcgacg
     1861 gtgcgcgaga cgtcgccgcc ctacggcgag agcccgccgg gcggctaccg caggcggcgc
     1921 cccgaggact acgaccgcac gctctgcctc ctgccgcgcg acgcgctgga cttcgtcctc
     1981 gccacgcagc cgaaggagtg gaagaagctt gagcagcacc acggcacagc ggtgcgggag
     2041 cagttcatca agcgcctcgc cgccgagatc gcacggcgcg gcgcgctcga cgtgctgcgc
     2101 aacggcctca aggactcggg ctgcaagttc aggctcgcgt acttccgccc cgcgagcggg
     2161 ctcaacgagg agacgcggcg gctgcacgcc gccaacctct tctccgtcgt tcgccagctc
     2221 cactacagca cgaagaacga gaagagcctc gacctggtgc tcttcctcaa cggcatcccg
     2281 atcttcaccg ccgagctcaa gaacccgctc accgggcagg acgtcgagga tgcgatccgg
     2341 cagtacaaga ccgaccgcga cccgcgcgag ccgctcctcg cctacggccg ctgcctcgcg
     2401 cacttcgcgg tcgaccccga cctcgtctac gtgacgaccc gcctcgcggg gacgaaaacc
     2461 cgcttcctgc ccttcaacca gggcaggttc ggcggcgccg gcaacccgcc cgtgccaccc
     2521 acgcggaagg gctacgcgac gtcgtacctc tgggaggaga cctgggcgcg cgacagcgtg
     2581 ctcgatctcg tgcgccagtt catccacgaa gtggaggagg aagacgacaa gggcaggaag
     2641 acgggcgcgc gctacatcat cttcccgcgc taccagcagc tcgactgcgt gcgcaggctc
     2701 gtcgccgacg cccgcgcgcg cggcgcgggg cagcgctacc tcgtccagca ctcggccggc
     2761 agcggcaaga gcttcaccat cgcctggctc gcccaccagc tctcgacgct gcacgacgcc
     2821 agcgaccggc gcgtcttcga ctcgatcgtg gtgatcaccg atcgccgcgt gctcgaccgc
     2881 cagctccaga cgacgatgcg ccagttcgag cagacgctcg gcgtggtcga gaacatcgac
     2941 accacctcac gccagctcaa ggaggccctc gagtcgggca agaccatcat cgtcacgacg
     3001 ctccagaagt tcccggtgat cgccgcggag atcggcgagc tgccgggcag gcgcttcgcc
     3061 gtcatcgtgg acgaggcgca ctcctcgcag tcgggcgaga gcacgaagag cctcaaggcc
     3121 gtgctcgcct ccggctcgct cgaggcggcg gaatcggagg aggcgggggc ggcgacgccg
     3181 gaagaggagc tggagaacac ggtcctcgcc gagatggagc gacgcgggca cctgccgaac
     3241 ctctcgacct tcgccttcac cgccacgccg aaaccgaaga cgctggagct gttcggcgtg
     3301 aagcgcgccg acgggaagtt cggtccgttc cacctgtaca gcatgcggca ggcgatcgag
     3361 gaggggttca tcctcgacgt gctggcaagc tacacgacct acacggccta ctggcgcctg
     3421 ctgaagacga tcgagagcga cccgcgctac gacaagaaga aggccgagta cctgctcaag
     3481 tcgttcgtgg agctgcaccc gcacgcaatc gccgagaagg tgaagctgtg cgtcgagcac
     3541 ttcgcggcgc acgcacaggg cgagatcggc gggaaggcca aggcgatgat cgtgacgcgc
     3601 tcgcgcctgc acgccgtgcg ctacaagctc gcggtggaca agtacgttgc cgagcgcggc
     3661 tacccgttca aggcgctggt ggcgttctcc gggacggtgc aggacggcgg gcaggcctac
     3721 accgaggcca acatgaacgg cgtccccgag acgcagaccg ctggaacctt cgagcgcccc
     3781 gactaccggt tcctcatcgt cgccaacaag ttccagaccg gcttcgacca gccgctgctc
     3841 cacaccatgt acgtggacaa gaagctcggc ggtgtgaacg cggtgcagac gctctcgcgc
     3901 ctgaaccgca cgcacccgga gaagaagaat acgctggtgc tcgacttcgc caacagcgcc
     3961 gacgagatcc agagagcttt cgagccctac tacgagacca cgctgctctc ggaggcgacc
     4021 gatccgaacc tgctctacga actgcagggc cgactcgcga agttcccggt gtacaccgag
     4081 gccgatgtca acgccttcgc gaagctctac ttcgatcgcg gtgcgacgca ggaccagctc
     4141 tacgccgtcc tggccccgct ggtggcgcgc tggggacagc tgcctgagga cgagcgcctc
     4201 gagttccggg gccagctcac cgattacgtc cggctctacg cgttcctcgc ccaggtgctg
     4261 accttcaccg acgccgacct cgagaagctc tacgtcttcg cgcgccacct gcgccgcctg
     4321 ctgcccgcgg accgcgacgt gctgccgcgc gaggtgcagc agaacatcga catggagtcg
     4381 taccggatcc agcagaccgg aagcgggaag atcgcactgg agcgcaagcc gggcgtggtg
     4441 gatccggcgg ggagccggac gtctcacggc gccgcgccgg aggaactcga atcgctctcg
     4501 cgcatcatcg ccgaactgaa cgagcgcttc gggctcaacc ccgaggtcat cacctcctgc
     4561 gacgccgtgg tccgcatccc cctgtttggc ttaaagaact cactcaatgt ggtcagcgcc
     4621 ttctccatcg caagctatac g
//
LOCUS       DAOSIF010000227         4624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16501_length_4624_cov_3.428978, whole genome shotgun sequence.
ACCESSION   DAOSIF010000227 DAOSIF010000000
VERSION     DAOSIF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16501_length_4624_cov_3.428978"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLT35_08380"
     CDS             complement(<1..854)
                     /locus_tag="PLT35_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29283.1"
                     /translation="MGPSRRRFLHDSLATVGGLALAAGRATGASSHPEDHEPAYLRLA
                     RQGRLREVERQLREILAACRLCPRSCGIDRRKGKTGVCSSTARLKVASHGPHFGEERP
                     LVGSGGSGTIFFSNCNLLCCYCQNWEINHRGDGRVVGEMDVADMMLDLQRKGCHNVNL
                     VTPTHVAPQIVGALCCAVDRGFRLPLVYNTGGYDSLDVIRALDGIVDIYLPDFKYQDG
                     ALAAKYSSGAADYPEVAAAAIKEMHRQVGTLTVDRRGIATRGLIIRHLVLPHNIGGEK
                     RVNFLAVM"
     gene            complement(891..1541)
                     /locus_tag="PLT35_08385"
     CDS             complement(891..1541)
                     /locus_tag="PLT35_08385"
                     /inference="COORDINATES: protein motif:HMM:NF021512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HOG29284.1"
                     /translation="MDRDGSFTAFAGTRQVASGDLRSVVLALKSRLDRGDEGAVLVFD
                     DRSGAELDFDLRGSTAEVLERLNSHPASAPGAEDASGPPERQARPGPGRPRLGVVSRE
                     ISLLPRHWEWLGRQRGGASGALRRLVDEARKADRGQERAQMAWEAAGRFMWAMGGNLP
                     GFEDAARALHRKDRARMSELIRAWPEDVRAHATRLAEAAEALERELSEAGDARPPV"
     gene            complement(1655..2077)
                     /gene="arfB"
                     /locus_tag="PLT35_08390"
     CDS             complement(1655..2077)
                     /gene="arfB"
                     /locus_tag="PLT35_08390"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOG29285.1"
                     /translation="MLQVTPEYVVPERELRERFVRSSGPGGQNVNKVATAVELRFDVG
                     ASALPADVKRRLLALAGTRASAEGIILVDSRAFRTQARNREAARARLAALIRQAFRRP
                     KSRTKTTPTAASRERRLAEKARRSRVKARRASIETDDD"
     gene            2076..3713
                     /locus_tag="PLT35_08395"
     CDS             2076..3713
                     /locus_tag="PLT35_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29286.1"
                     /translation="MPPPLYHARCYNPPGPASHVKRGTQPGRTSGARRRRNAGSSSPG
                     EPRKKVTMRALVICFMPVAALLGSCGNPSTDQARPMLRPYESSSKLFVLFKPSDWKVT
                     EDARPDSLRVLVRDPAGTSMVDFRWARSDARVSDALAALLGARKALALEHGDVALSEV
                     FASKDGSRATAAVRWGSGAGSVAGRLYIEASAGSHSVQGYFAPSSRLAAQRSLLLNVM
                     ASFAFSKAPAAQGARAAAPAPPPPVQAALTARAAPDGSLSLRVPADWSFLAGRGRVIA
                     ASRDGGLGFIFTAFAGNPLVPQATVAQGIIPTPYQPPAETLGYVFSAFRNRQFRIHTA
                     ASDQGTARECMARMGGACDAQDFQVSYTSPEGTACVGGFKVINFPPSPITGLWNTIVA
                     GIWGPERDFARYLPMLEQIAGSFAVNDQYARRYVQNGLENLRRLQQKTQAAMQDLNAS
                     RAQNQQDWEARQERKAFMDSGWDDYRRGHSYWVSELEGGKVYATDSGGTEDTVTGQYY
                     EGRPYSWVNFEGRNPRHPSETMREINSYELKKLEERQ"
     gene            3766..4434
                     /locus_tag="PLT35_08400"
     CDS             3766..4434
                     /locus_tag="PLT35_08400"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG29287.1"
                     /translation="MRTSVVIPSRNEGATVVALADAVRAHCDEVIVVDGHSTDGSPAR
                     LREAGVTVVEDGGLGKGDAIRRGLAAAGGEVIVTMDADGSHDPADIRALVAPIGAGEA
                     DLVIGCRMRGGSDEFAGTWTMFVRLWGNNFLTQVINTRFGTALTDSQNGFRAIRAAAV
                     AQLGLRENRHSIELEMVLRALKRGLRVCQVPAHEYARKAGKSSLSVVRQTPTFLRCLL
                     RNIW"
BASE COUNT          713 a         1620 c         1608 g          683 t
ORIGIN      
        1 tacatgacag caaggaaatt gacgcgcttc tcgccgccaa tgttgtgcgg cagcaccaag
       61 tgccggatga tcaatccccg ggtggcgata ccgcgtcgat cgacagtcag tgtgccaacc
      121 tggcggtgca tctccttgat cgccgccgcc gccacctctg gatagtcggc ggctccgctc
      181 gagtacttcg ccgccagcgc cccgtcctgg tacttgaagt ccggcaggta gatgtcgacg
      241 atgccgtcca gggcgcggat cacgtcgagg ctgtcgtagc cgcccgtgtt gtagaccagc
      301 ggcaggcgga agccgcggtc gacggcgcag cagagcgccc cgacgatctg gggcgccacg
      361 tgggttggcg tgacgagatt gacgttgtgg cagcccttcc gctgcaggtc gagcatcatg
      421 tccgcgacgt ccatctcgcc gacgacgcgg ccgtccccgc ggtggttgat ctcccagttc
      481 tggcagtaac agcagagcag gttgcagttc gagaagaaga tagtgccgct gccgccgctg
      541 cccacaagcg gccgctcctc cccgaaatgc ggcccgtggg aggccacctt caggcgcgct
      601 gtcgaactgc acacgccggt cttgcccttg cggcggtcga tgccgcacga gcgcggacag
      661 aggcggcagg ccgcgaggat ctctcgaagc tgccgttcga cctctcggag ccgaccctga
      721 cgggcgaggc gcagataagc gggttcgtgg tcctccgggt gactcgaggc acccgtcgca
      781 cggccggcgg ccagcgcgag gcctccgaca gtcgcaagcg agtcgtggag gaaccggcgc
      841 cggctcggac ccatgtcatc ctccccaggg acgcacgtcc cggcgggatc tcacaccggc
      901 ggccgtgcat ctccggcttc ggagagctcc cgctccaagg cttcggcggc ttccgcgagt
      961 cgagtagcgt gggcccggac gtcctccggc caggcccgga tgagctccga catgcgggcc
     1021 cggtccttcc ggtgcaacgc acgcgcggcg tcctcgaatc ccggcaggtt gccgcccatc
     1081 gcccacatga acctgcccgc ggcctcccaa gccatctggg cgcgttcctg gcccctgtcg
     1141 gccttcctcg cttcgtcgac gaggcgccgg agggcgcctg aggccccgcc tctctgtcgc
     1201 ccgagccact cccaatggcg cggcagcagg gaaatctcac ggctgaccac gccaagcctg
     1261 ggtcgtcccg gacccgggcg cgcctggcgt tctggcgggc ccgaggcgtc ctcggccccg
     1321 ggagcgctgg ccggatggct gttcaggcgc tccagcacct ctgctgtcga gccccgcagg
     1381 tcgaaatcga gttcagcccc agatcgatcg tcgaagacga ggacggctcc ctcatcgccc
     1441 ctgtcgagcc tgctcttgag tgccagcacg accgagcgaa ggtctcccga cgcgacttgc
     1501 cgcgtacctg cgaaggcggt aaacgaaccg tctcgatcca aatccaaccc ggattcccag
     1561 acaggctaat attacccgga tgaaatcagc ctgtcaataa ctcccggacg aaatggcctg
     1621 ccgtgctaag gaaggcgtgg acagaagaac cgagctagtc gtcgtcggtc tcgatggaag
     1681 cgcggcgggc cttgacccgg cttcgccgag ccttttcggc aaggcgccgc tcgcgcgatg
     1741 cggccgtggg cgtggtcttc gtgcgagatt tcggcctgcg gaacgcctgg cgtatgaggg
     1801 cagcgaggcg ggcgcgggcg gcctctcggt tcctcgcctg tgtccgaaag gccctgctgt
     1861 ccaccaggat gatgccctcg gcagacgctc gcgtgcctgc cagtgcgagg aggcggcgct
     1921 tcacgtccgc cggcagcgcc gacgcgccga cgtcgaagcg cagttccacc gcggtggcca
     1981 ccttgttgac gttctgcccg ccgggccccg acgagcgaac gaagcgctcg cgcagttcgc
     2041 gttcggggac cacgtactcc ggtgtgacct gcagcatgcc gccgccactg taccacgcac
     2101 ggtgctacaa tccgcctggg ccggcgtccc acgtcaagcg cggcacgcag cccgggcgca
     2161 ccagcggcgc ccggcgccgg cggaacgccg gcagctccag cccgggcgag ccgcgcaaga
     2221 aggtcactat gcgcgccctc gtcatctgct tcatgccggt cgccgcgctg ctcggcagct
     2281 gcgggaatcc atcgaccgat caggccaggc ccatgctgcg tccctacgaa tcctccagca
     2341 agctgttcgt gctcttcaag ccgtccgact ggaaggtgac cgaggacgcg cgtccggaca
     2401 gcctgcgcgt gctcgtacgg gatcctgcgg ggacttccat ggtggacttc cgctgggcgc
     2461 ggagcgacgc acgcgtttcc gacgcgcttg ccgcgctgct gggagccagg aaggcgctgg
     2521 ccctcgagca cggggacgtg gcgttgagcg aggtgttcgc gtcgaaggac ggcagccggg
     2581 caacggcggc cgtccgatgg ggaagcggcg cgggcagcgt cgccggcagg ctgtacatcg
     2641 aagcgtcggc agggtcgcac tcggtccagg gctacttcgc gccatcatcc cgcctggcgg
     2701 cgcaacggtc gctgctgttg aacgtgatgg cgagcttcgc cttctccaag gcaccggcgg
     2761 cacaaggtgc ccgcgcggcg gcgccggcgc cgccgccgcc cgtgcaggcg gcgctgacag
     2821 cgcgcgcggc cccggatggc agcctgtcgc tgcgcgtgcc cgccgactgg agcttcctcg
     2881 cgggccgggg acgggtgatc gcggcctcgc gggacggcgg actggggttc atcttcaccg
     2941 cgttcgccgg caacccgctg gtcccgcagg cgacggtggc gcagggcatc atcccgacgc
     3001 cataccagcc gcccgcggag acgctcggct acgtcttcag cgcgttcagg aatcgccagt
     3061 tccgcattca cacggccgct tccgaccagg gaacggcgcg cgagtgcatg gcgcggatgg
     3121 gcggcgcgtg cgacgcgcag gacttccagg tgagctacac gtcgccggag ggcacggcct
     3181 gcgtcggcgg gttcaaggtg atcaacttcc cgccgtcgcc gatcacgggc ctctggaaca
     3241 ccatcgtcgc cggcatctgg gggcccgagc gggacttcgc ccgctacctg ccgatgctcg
     3301 agcagatagc aggcagcttc gccgtcaacg atcagtacgc acgccgctac gtccagaacg
     3361 ggctcgagaa cctgcgccgc ctgcagcaga agacgcaggc tgcgatgcag gacctgaacg
     3421 cctcccgggc ccagaaccag caggactggg aggcccgcca ggagcgcaag gccttcatgg
     3481 actcgggctg ggacgactac cgccgagggc acagctactg ggtgtcggag ctcgagggcg
     3541 gcaaggtgta cgccaccgac tcgggcggca ccgaggacac cgtcaccggc cagtactacg
     3601 agggcaggcc ctacagctgg gtgaacttcg aaggccggaa cccgcgccat ccgtccgaga
     3661 cgatgcgcga gatcaacagc tacgaactga agaagctcga ggagcggcag taggcgcgcc
     3721 gcctcgtgcg tccggccgcg cgccggctcg gggtatcctg tatccgtgcg cacgtcggtc
     3781 gtcatcccgt cccgcaacga aggcgccacg gtcgtcgccc tcgcggacgc cgtccgcgcg
     3841 cattgcgacg aggtgatcgt cgtggacggt cacagcaccg acgggtcgcc cgcgcgcctg
     3901 cgggaggccg gcgtcaccgt cgtcgaggac ggcgggctgg gcaagggtga tgcgatccgg
     3961 cgcggcctgg cggccgccgg cggcgaggtg atcgtcacga tggacgcgga cgggtcgcac
     4021 gacccggcgg acatccgggc gctcgtcgca cccatcggag ccggcgaggc ggacctcgtg
     4081 atcggctgcc ggatgcgggg cggcagcgac gagttcgccg gcacctggac gatgttcgtg
     4141 cggctctggg gcaacaactt cctgacacag gtcatcaaca cgcgcttcgg caccgcgctc
     4201 accgacagcc agaacggctt ccgcgcgatc cgcgcggccg ccgtcgctca gctcgggctg
     4261 agggagaacc ggcactcgat cgagctcgag atggtgttga gagccctcaa gcgcggcctg
     4321 cgcgtctgcc aggtgcccgc gcacgagtac gcccgcaagg cggggaagtc gagcctgtcg
     4381 gtcgtccggc agacgccgac gttcctccgc tgcctgctcc gcaacatctg gtagagacgt
     4441 ccgccggcgg ggtggccgtc ccgcccggca gcgcgcgctc gggcgcgcgc aatctacggc
     4501 cggcggcgcg cgtagacgac catctccggg ttcaccttgt cgaggttggt gagaggggac
     4561 tcctcgtcgc cgcgcatcgg cgcctcgtac tgcagaagtg cccacgcggg gagcggcgca
     4621 cgat
//
LOCUS       DAOSIF010000228         4614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16540_length_4614_cov_4.604519, whole genome shotgun sequence.
ACCESSION   DAOSIF010000228 DAOSIF010000000
VERSION     DAOSIF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16540_length_4614_cov_4.604519"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="PLT35_08405"
     CDS             <1..1804
                     /locus_tag="PLT35_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOG29288.1"
                     /translation="QPLSAKQARIEKQAAERRQKEADLWVARTMAKMTLDEKIGQLIF
                     TSFNAGYMSTDSAEFDRLLHLVRDVRVGGIHVFGSAEAFPQVMLNPVWGAGPASRKGD
                     PYAAAAVLNRLQAESAVPLLTSGDFEGGAAYILNGATRLPRAMVIAATGDEQMAFNAG
                     VVGATEARAVGVNIDFYPVVDVNNNPRNPIINLRSFGEDPAFVSKMAVAYIKGIQSTG
                     AFATAKHFPGHGDTNVDTHLGLAVINHPRAHLDEVELVPFRAAIAAGVDAVMSTHIIL
                     PALDPSPGIPATLSRPILTGLLRDELKFSGLVFTDSMTMDAISKMFPHDKAAAMAVRA
                     GADFVLHSPDDDAAFRGIKAAVEAGEIPMAQVDASVERILRAKARLGLHVKRIVDVEA
                     IAGKFGTRAHAAVAQEINDRGITLVRDERNQVPLSVPRDADLLYLSVLDYASGWREGV
                     PGRTIVPELKRRWPNLTSIEITDRTTASEYELIRAISRRAGAVVAGVFVRIASFSGRM
                     DLSQPQIALLDSIAAQDKPFVTVCFGNPYTATFLPKLPAVLLGYEFSDFTERAAVKAL
                     AGEIAVRGRLPISLPGMFPIGHGLARAARPGAGE"
     gene            2005..2081
                     /locus_tag="PLT35_08410"
     tRNA            2005..2081
                     /locus_tag="PLT35_08410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2039..2041,aa:Arg,seq:acg)
     gene            complement(2428..>4614)
                     /locus_tag="PLT35_08415"
     CDS             complement(2428..>4614)
                     /locus_tag="PLT35_08415"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HOG29289.1"
                     /translation="DAHVAWGATPSPLPRVEGSRWVVRHRAGATRYQHAVEGLEQEFE
                     VFVPPDDPVKVSVLTLRNTSETSRRLSVFGYAEWRLGPPRQGERRFVITEADERAGAI
                     FARNPYNSEFAGRVAFLRATAAPASYTCDRGDFIGRQRTPANPAGLGRGRLANRSGAG
                     LDPCAALHVPIVIPPGETRRLAFVLGQGRDAAEAADLAARYASIESVEAAGERCNRFW
                     DETLGAIQVKTPDDSFDLIVNRWLLYQSLSCRMWARSGPYQPGGAWGFRDQLQDALAL
                     VYTRPDICRAHLLEAASRQFVEGDVQHWWHPPTGRGTRTRCSDDLLWLPYAVAAYVSR
                     TGDEGVLDEAVRFLEAPPLAPDQQEAYFQPSVSAQSASLFEHCCRAVDRSLVYYGAHG
                     LPLMGSGDWNDGMNRVGYEGRGQSVWLAWFLIVVLNEMAGLADRRELPDVAQRYRSQV
                     EWLAGMAELAWDGDWYRRAYFDDGTPLGSAQNDECRIDSLTQSWAVLSGAAQLRRAAR
                     AMEAVRAHLVRRDAQLVLLLTPPFDRTPLDPGYIKGYLPGLRENGGQYTHAALWAVIA
                     LARLGQGDEAMELFHMVNPINHTRTRDEVDRYKTEPYVVAADVYAHPQHAGRGGWSWY
                     TGSAGWMYQAAVEALLGLRRRGQSFALDPCIPARWEGFSMEWRVGRTTYRIEVASPEQ
                     RGRGVGQVHLDGVPVDPRKIPLVDDGRLHEVRAVLGEVGTTDSAAV"
BASE COUNT          663 a         1655 c         1660 g          636 t
ORIGIN      
        1 ccagcccctc tcggcgaagc aggcgaggat cgagaagcag gcggccgaaa ggcggcagaa
       61 ggaagccgat ctctgggtcg cgcgcacgat ggcgaagatg acgctggacg agaagatcgg
      121 ccagctgatc ttcacctcgt tcaacgcggg ctacatgagc accgacagcg cggagttcga
      181 ccgcctgctg cacctcgtgc gcgacgtgag agtcggcggc atccacgtgt tcggcagcgc
      241 ggaggcgttt ccgcaggtga tgctcaatcc cgtgtggggc gccgggccgg cctcccgcaa
      301 gggcgatccg tacgcggccg cggcggtcct gaaccgcctg caggccgagt cggcggtccc
      361 gctcctcacg tcgggcgatt tcgagggcgg cgcggcctac atcctgaacg gcgccacgcg
      421 gctgccgcgc gccatggtca tcgcggcgac cggcgacgag cagatggcct tcaacgcggg
      481 cgtggtcggc gcgaccgagg cgcgcgccgt cggcgtcaac atcgacttct atccggtcgt
      541 cgacgtcaac aacaacccgc gcaacccgat catcaacctg cgctcgttcg gcgaggaccc
      601 cgcgttcgtg tcgaagatgg cggtggccta catcaagggc atccagtcca ccggcgcctt
      661 cgccaccgcg aagcacttcc cgggccacgg cgacacgaac gtcgacacgc acctcgggct
      721 cgccgtcatc aaccaccccc gcgcgcacct ggacgaggtc gagctggtgc ccttcagggc
      781 ggccatcgcg gcgggcgtcg acgcggtgat gtccacgcac atcatcctgc cggccctcga
      841 cccgtcgccc ggcatcccgg ccacgctgag ccgcccgatc ctcacgggcc tgctgcgcga
      901 cgagctgaag ttctcgggcc tcgtcttcac cgactcgatg acgatggacg cgatttcgaa
      961 gatgttcccg cacgacaagg ccgccgcgat ggcggtgcgc gccggcgcag acttcgtcct
     1021 gcactcgccc gacgatgacg cggcgttccg gggcatcaag gcggcggtcg aggcgggtga
     1081 gatcccgatg gcgcaggtcg acgcgtcggt cgagcgcatc ctgcgggcga aggcccggct
     1141 ggggctgcac gtgaagcgga tcgtggacgt cgaggccatc gcggggaagt tcggcacgcg
     1201 cgcgcacgcg gcggttgcgc aggagatcaa cgaccgcggc atcacgctcg tcagggacga
     1261 gcggaaccag gtgccgctgt ccgtgccgcg cgacgcggac ctgctttact tgtcggtgct
     1321 cgactacgcg tcgggctggc gggagggcgt gccgggtagg acgatcgtgc cggagctgaa
     1381 gaggcggtgg ccgaacctga cgtccatcga gatcacggat cgcacgaccg cgtctgagta
     1441 cgagctcatc cgggccatct cgcgccgcgc gggcgccgtc gtcgcgggcg tgttcgtgcg
     1501 catcgcgtcg ttcagcggcc ggatggacct gagccagccg cagatcgcgc tgctcgattc
     1561 gattgccgcc caggacaagc cgttcgtcac cgtgtgcttc gggaatccct acaccgccac
     1621 gttcctgccg aagctgccgg cggtgctcct cggctacgag ttctcggact tcaccgagcg
     1681 ggcggccgtg aaggcgctgg ccggggagat cgcggttcgg ggcaggctgc cgatctcgct
     1741 gccggggatg ttcccgatcg gccacgggct ggcgcgggcg gcgcggccgg gagcggggga
     1801 ataggaacag gggattgacg agatcggaac ggggcgcgag acagccggct ggcggcggcg
     1861 cccgggcatc ccgggcgtcc ggcgggaaat gaccggggag gcgccgcgga acccggcgaa
     1921 ggcccgggtc ttcacccctg gcgccgtcgt ggtatattcg attgttccgg cggatgtact
     1981 cgttgccgcc gggcaacggc ccgtgcgccc ttagctcagc tggatagagc gtctggctac
     2041 gaaccaggag gccgcaggtt cgagtcctgc agggcgcacc attgctctcg acaccctgag
     2101 cgggcccgcg cgttcgccgc gggccgcgcg tcctctcgta tgtcgcgcgc aggctgggcg
     2161 gatcgatccg accaggcccg cgcgttcatc gccgtggcgg tgcagctcga acggccccgc
     2221 ggacgggcgg cggccgacga cggcacgtca gggcggaacc ggtgcagccg cccgcggcat
     2281 cgccatcccg gaaccgacac gagcggacct cccctgctaa cggaatgctg cgggacgcgg
     2341 cccgcgccct cgcccgacac ccgggacagc ccgcggcgcc gtgccctgtt cgtcgctgcc
     2401 ggtggacgcc gccgctgccc cgcagcccta cacggctgcg ctatcggtgg tgccgacctc
     2461 gccgaggacg gccctcacct cgtggagcct gccgtcgtcc accaacggga tcttccgggg
     2521 atcgacgggg acgccgtcga ggtggacctg gcccacgccg cggccgcgct gctcggggct
     2581 ggcgacctcg atccggtagg tcgtccggcc gacgcgccac tccatggaga agccttccca
     2641 gcgagccggg atgcacgggt cgagcgcgaa cgactggccg cgccgccgca gaccgagtag
     2701 cgcctcgacg gcggcctggt acatccaccc ggccgacccc gtgtaccagc tccacccgcc
     2761 gcgccccgcg tgctgggggt gcgcgtacac gtcggcggcc accacgtagg gctcggtctt
     2821 gtagcggtcg acctcgtcgc gcgtgcgggt gtggttgatc gggttgacca tgtggaacag
     2881 ctccatcgcc tcgtcgccct gcccgaggcg ggcgagcgcg atcacggccc acagcgccgc
     2941 gtgcgtgtac tgcccgccgt tctcgcgcag gcccggcagg taccccttga tgtagccggg
     3001 gtcgagcggc gtgcggtcga aggggggcgt cagcagcagc acgagctgcg cgtcgcgtcg
     3061 gacgaggtgc gcgcgcaccg cttccatcgc ccgcgcggcg cgccgcagct gggctgcgcc
     3121 cgagagcacg gcccacgact gggtcagcga gtcgatccgg cactcatcgt tctgcgccga
     3181 cccgagcggc gtcccgtcgt cgaagtacgc gcggcggtac cagtcgccgt cccaggcgag
     3241 ttcggccatg ccggccagcc actcgacctg gctccggtag cgctgcgcca cgtcggggag
     3301 ctcgcggcga tcggccagcc ccgccatctc gttcaacacg acgatgagga accaggcgag
     3361 ccagacgctc tggccccgac cctcgtagcc gacgcggttc atgccgtcat tccagtcgcc
     3421 cgatcccatg agcgggaggc cgtgcgcgcc gtagtagacg agcgagcgat cgacggcgcg
     3481 gcagcagtgc tcgaagagcg aggccgactg cgccgacacg gacggctgga agtaggcctc
     3541 ctgctggtcg ggcgccagcg gcggcgcctc gaggaacctg acggcctcgt cgagcactcc
     3601 ctcgtcgccg gtccgcgaga cataagccgc caccgcgtag ggcagccaga gcaggtcgtc
     3661 cgagcaccgg gtgcgcgtcc cgcggccggt aggcgggtgc caccagtgct gcacgtcgcc
     3721 ctcgacgaac tggcgcgagg ccgcctcgag caggtgcgcg cggcagatat cgggccgcgt
     3781 gtagaccagc gccagcgcgt cctgcagctg gtcgcggaat ccccacgcgc cgccgggctg
     3841 gtagggcccg ctcctcgccc acatccggca gctcagcgac tggtacagca gccacctgtt
     3901 cacgatcagg tcgaacgagt cgtccggcgt cttcacctgg atcgcgccga gcgtctcgtc
     3961 ccagaagcgg ttgcagcgct ccccggccgc ctcgaccgac tcgatcgacg cgtagcgcgc
     4021 ggccaggtcg gcggcctcgg cggcgtcgcg cccctggccg aggacgaacg ccagccgccg
     4081 cgtctcgccg ggcgggatca cgatcggcac gtgcagcgcg gcgcagggat ccagcccggc
     4141 gccgctccgg ttggcgaggc ggccgcggcc aagccccgcc gggttggccg gcgtgcgctg
     4201 gcggccgatg aagtcgccgc gatcgcacgt gtacgaggcg ggcgccgcgg tggcgcgcag
     4261 gaaggcaacg cggccggcga actcgctgtt gtacgggttg cgcgcgaaga tcgcgccggc
     4321 tcgttcgtcc gcctcggtga tgacgaagcg gcgctcgccc tggcgcggcg gcccaaggcg
     4381 ccactcggca tatccgaaca cgctcagccg gcgcgaggtc tcagaggtgt tcctgagcgt
     4441 cagcaccgac actttcaccg ggtcgtcggg cggcacgaag acctcgaact cctgctcgag
     4501 gccttcgacc gcgtgctgat atcgggtcgc ccccgcgcgg tgccgcacga cccagcggct
     4561 cccctccacg cgcggcagcg gcgacggcgt cgcgccccag gcgacgtgcg cgtc
//
LOCUS       DAOSIF010000229         4611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16552_length_4611_cov_3.339991, whole genome shotgun sequence.
ACCESSION   DAOSIF010000229 DAOSIF010000000
VERSION     DAOSIF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16552_length_4611_cov_3.339991"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="queC"
                     /locus_tag="PLT35_08420"
     CDS             <1..633
                     /gene="queC"
                     /locus_tag="PLT35_08420"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOG29290.1"
                     /translation="TAGAIARAEGFGLYALTVAYGQRHRVELEAARRVARALGVVRHV
                     ELPLDLAAFGGSVLTGAGDVPRDRAIGEGGIPATYVPARNTVFLSLALAWAETLGASD
                     IVIGVNAVDYSGYPDCRPEFIRAFESLAALATKAGAGGARFRVLAPLASLSKADIIRR
                     GLALGLDYGLTHSCYDPRPGGRPCGRCDSCVLRAKGFAEAGARDPLVDGA"
     gene            659..1867
                     /locus_tag="PLT35_08425"
     CDS             659..1867
                     /locus_tag="PLT35_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOG29291.1"
                     /translation="MTERIYYGDPACLEFDAAVLDCTADGGRTIVVLDRTAFYPTSGG
                     QPHDIGWLGGVPVSDVIELDDDRIGHVVDSPIPAGSAVRGRIDRARRFDHMQQHTGQH
                     ILSAAFDRLHGARTVGFHLGALVSTIDLDKVLGAEPLADAEAEANRVVWEDRPLRILT
                     ASGEEGALLGLRKEAVRSGPLRVIEVERFDRSACGGTHVARTGEVGVIVLRSWEKFRG
                     GMRVEFVCGARALREFRALRDAVAGSLRFLSVPPQELPSAIEGAQAENKELRRAIRGL
                     TERLAAHEAAALVGRARPAGPIRLVVESLDDWDQTGLTALASAAAAQPGVVAALLSAG
                     PDPAVAAIACAPDTGVNAAVVLRRLAERFGGKGGGKPGLAQGGGLTGERAAMLAALEE
                     IIAASLPPAP"
     gene            complement(1836..2588)
                     /locus_tag="PLT35_08430"
     CDS             complement(1836..2588)
                     /locus_tag="PLT35_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29292.1"
                     /translation="MAASAALYFFVAAAAAGALSIVRPIRRLGIRSRKAGWLVLLAGA
                     AGAQYVLGRPALATYVTTPETKLDEFAPIYQFSEIQSIPVRAPAGRVYESLFAVTAAE
                     VPLYRELAWIRRGGASGPESVLNPSDDVPLVDVATRTSFLQLAAVPGRELVLGAVVMA
                     PPGVRLAAAPTPAAFQALRQPGFAKAVMGFVIEAQDSGWCRLRSEIRVHATDPASRDT
                     FSTYWKVITPGSQLMHLMWLRAIKVRAEATRR"
     gene            complement(2605..4113)
                     /gene="dacB"
                     /locus_tag="PLT35_08435"
     CDS             complement(2605..4113)
                     /gene="dacB"
                     /locus_tag="PLT35_08435"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOG29293.1"
                     /translation="MKRALAAAFLAAALTAGCAAHPAPAVSPRPPRPPKPGVSALRAD
                     LASCFRTPALRGAVSGVLVRSLKTGETLAAINPGTFLMPASNMKVVTAAAAAERLGWD
                     YTFTTKVVAAGPIEGGVLKGDLVIVGSGDPALGGRPTPEGFALVDRWAEAIRAAGVSA
                     VEGRIVGDDNAFEDEGLGSGWAWDDLAYGYAAPVGGLNFNENTARLTFAPGAARGDPV
                     TVEVRPDGSGLQVDSTVTTADPDGEVDLRIRRLPGSARLGVRGSVPAGKTDVTQAVSV
                     DNPTEFLARALKLALAGRGVTVSGEAIDGDALAAPVDIASASTLVTHVSPPLADIVKV
                     LLKASQNLYADTLLKALGRTASGGPASTAAGKLAVRDVLQAWGIGPDQYIQADGSGLS
                     RYNYLSADVLVAVLTRMYYDERHRDAFMAALPVAGVDGTLANRMKGTRAEGNARAKTG
                     SIANTRSLSGFVASASGEPLVFSMIVNNFNAAPSDALDAIDRAVVRLAEFRR"
     gene            complement(4110..>4611)
                     /locus_tag="PLT35_08440"
     CDS             complement(4110..>4611)
                     /locus_tag="PLT35_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOG29294.1"
                     /translation="GSRYARDASKRLVVTVEPAILDDLKKKADDYRPRDVFDFRSFTA
                     TRLEVTRGASTVAFERTTDKDGAATWRQVQPAKEVESSSVLAALGALSGLSVERYVDA
                     KTRTGADAPVAVVTAAFDEGKKTERVAFGKVGDEVFAVREGEPGGAKVDAARFDEALK
                     ALDAVK"
BASE COUNT          610 a         1662 c         1695 g          644 t
ORIGIN      
        1 acggctggcg cgatcgcgcg agcggagggc ttcggtctgt acgcgctgac cgtggcgtac
       61 ggccagcgac accgcgtcga gctcgaggcc gcccggcgcg tggcccgcgc cctcggggtc
      121 gtgcgtcacg tcgagctgcc actcgacctc gcggccttcg gcgggtcggt cctcacgggg
      181 gccggggacg tgcctcggga ccgggcgatc ggcgagggcg gcatcccggc gacgtacgtg
      241 ccggcgcgca acacggtctt cctctcgctg gcgctggcat gggcggagac cctgggcgcg
      301 agcgacatcg tcatcggcgt caacgccgtg gactactcgg gctatcccga ctgccgcccc
      361 gagttcatcc gggccttcga atcgctggcc gcgctggcga cgaaggccgg ggcggggggc
      421 gcccgcttcc gcgtgctcgc gccgctggcg tcgctgtcga aggccgacat catccgccgc
      481 gggctcgccc tcggcctgga ttacggcctc acgcacagct gctacgaccc gcgccccggc
      541 gggcggccgt gcgggcggtg cgacagctgc gtgctgcggg cgaagggatt cgccgaggcg
      601 ggcgcgcgcg acccgctcgt tgacggcgcc tgacgcgcgc cggagggcag aacgacccgt
      661 gaccgagcgc atctactacg gcgatccggc gtgcctcgaa ttcgacgccg ccgtcctgga
      721 ctgcacggcc gacggcggcc gcacaatcgt cgtcctcgac cgtacggcct tctatcccac
      781 ctccggcggc cagccccacg acatcggatg gctcggcggc gtcccggtct cggatgtcat
      841 cgagctggat gacgatcgga tcggccacgt cgtcgactcg ccaatcccgg ccgggtcggc
      901 ggtccgcggc cggatcgacc gcgcccggcg gttcgatcac atgcagcagc acacggggca
      961 gcacatcctg tcggctgcct tcgaccggtt gcacggggcg cgcacggtcg gcttccacct
     1021 cggggcgctc gtgtcgacca tcgatctcga caaggtgctc ggcgccgagc cgctggccga
     1081 cgccgaggcc gaagccaacc gcgtggtgtg ggaggaccgg ccgcttcgga tcctgacggc
     1141 gtcgggcgaa gagggcgccc tgctgggcct gcgcaaggag gcggtccgga gcggcccgct
     1201 gcgcgtgatc gaggtcgagc gcttcgaccg ctccgcgtgc ggcgggacgc acgtggcccg
     1261 cacgggcgag gtgggagtca tcgtgcttcg ctcgtgggag aagttccgcg ggggcatgcg
     1321 cgtcgagttc gtgtgcggcg cccgcgccct tcgggagttc cgggcgctgc gcgacgcagt
     1381 cgccggaagc ctccggttcc tgtcggtgcc cccgcaggag ctgccctcgg ccatcgaggg
     1441 cgcccaggcc gagaacaagg agctgcggcg ggccatacgc gggctcaccg agcggctcgc
     1501 cgctcacgag gccgccgcgc tcgtgggacg cgcgcggcct gccggaccga tccgtctcgt
     1561 cgtcgaatcg ctggacgact gggatcagac ggggctcacg gccctcgcct cggccgccgc
     1621 ggctcagcct ggcgtcgtgg ccgcgctgct ctcggccggt ccggaccccg cggtggcggc
     1681 gatcgcatgc gcgccggaca caggggtgaa cgctgcagtc gtgctcagga ggctggccga
     1741 gcggttcggg ggaaagggcg gcggcaagcc cggcctggcc cagggcggcg ggctgaccgg
     1801 cgagcgggcg gcgatgctcg cagcgctcga ggagatcatc gccgcgtcgc ttccgcccgc
     1861 accttgatgg ccctcagcca catcaagtgc atcagctggc tgccgggcgt gatgaccttc
     1921 cagtaggtgg agaacgtgtc gcgcgaagcc gggtccgtcg catgcacccg gatttccgag
     1981 cgcagcctgc accagccgga gtcctgcgcc tcgatgacga agcccatgac cgccttggcg
     2041 aaacccggct gcctgagcgc ctggaacgcg gctggcgtcg gagccgcggc cagcctgacg
     2101 cccggtggag ccatgacgac ggcgccgagt acgagttccc gccccggcac ggccgccaac
     2161 tgcaggaacg acgtcctcgt ggccacgtcc accaggggga cgtcgtcgct ggggttcagc
     2221 acgctctcgg gcccgctcgc cccaccccgg cggatccagg ccagctcgcg gtacaggggc
     2281 acttcggcag ccgtcacggc gaagagcgac tcgtagacgc gccccgccgg cgcgcggacc
     2341 gggatcgact ggatctcgga gaactggtag atcggcgcga actcatccag cttcgtttcg
     2401 ggcgtcgtga cataggttgc gagcgcgggc cggccgagga cgtactgcgc gcccgcggcg
     2461 cccgcgagga ggacgagcca ccccgccttg cggctgcgga tgcccagccg gcggatcggg
     2521 cggacgatcg acagcgcgcc cgccgcggcc gccgccacga agaagtagag cgccgcggac
     2581 gccgccatgc gcgctgactc cgatctagcg ccggaactcc gccagccgca cgaccgcccg
     2641 atcgatcgcg tcgagggcgt ccgacggcgc ggcgttgaag ttgttgacga tcatcgagaa
     2701 gacgagcggt tcgcctgagg ccgaggcgac gaacccggac agcgaacgcg tgttggcgat
     2761 cgatccggtc ttggcccggg cgttgccttc ggccctcgtg cccttcatgc ggttggccag
     2821 cgtgccgtcc acgccggcga cgggcagggc ggccatgaac gcgtcgcggt ggcgctcgtc
     2881 gtagtacatg cgcgtcagca ccgcgaccag cacgtcggcg ctcaggtagt tgtacctcga
     2941 caaaccggag ccgtccgcct ggatgtactg atcggggccg attccccacg cctggagcac
     3001 gtcccgcacc gccagcttcc cggcggctgt ggaggccggc ccgcccgagg ccgtgcgacc
     3061 cagggccttc agcagcgtgt ccgcgtacag gttctggctc gctttcagga gcaccttgac
     3121 gatgtcggca agcggcggcg acacgtgcgt gacgagagtc gaggccgacg cgatgtcgac
     3181 gggcgccgcg agcgcgtcac cgtcaatcgc ctcgccggac accgtcacgc cccggccggc
     3241 gagcgccagt ttcagcgcgc gcgcgaggaa ctcggtcggg ttgtccaccg acaccgcctg
     3301 cgtgacgtcg gtcttgccgg ccggcaccga tccgcggacg ccgagccggg cgctgccggg
     3361 gagcctgcga atgcggaggt cgacttcgcc gtcgggatcc gccgtcgtca ccgtggagtc
     3421 gacctgcaag ccgctgccgt cgggcctgac ttcgacggtc acgggatcgc cccgggccgc
     3481 tcccggcgcg aacgtcagcc gtgccgtgtt ctcgttgaag ttgaggccgc cgaccggcgc
     3541 cgcgtagccg tacgcgaggt cgtcccaggc ccatccggag ccgaggccct cgtcttcgaa
     3601 cgcgttgtcg tcgccgacga tgcgcccctc gacggccgac acgccggcag cccggatcgc
     3661 ctcggcccag cgatcgacga gggcgaagcc ctcgggcgtg ggccggcctc cgagcgcggg
     3721 gtcgccgctg ccgacgatga cgaggtcgcc cttcaggacg ccgccctcga tcggcccggc
     3781 cgccacgacc ttcgtggtga acgtgtagtc ccagccgagg cgctcggcgg ccgcggcggc
     3841 ggtcaccacc ttcatgttcg aggcgggcat gaggaaggtg cctgggttga tcgcggcgag
     3901 ggtttcgccg gtcttcagcg accggaccag tacgccgctg acggcgccac gcagcgccgg
     3961 cgtgcggaag caggaggcca gatcagccct gagggcgctg acgcctggct tcggcggccg
     4021 cggcggccgt ggggagaccg caggggcggg gtgcgcggcg caccccgccg tgagtgcggc
     4081 ggcgaggaac gcggcggcaa gcgcccgctt cacttgacgg cgtccagggc cttgagggcc
     4141 tcgtcgaacc tggcggcgtc caccttggcg ccgcccggct cgccttcgcg cacggcgaag
     4201 acctcgtcgc ccaccttgcc gaaggcgacg cgctccgtct tcttgccttc gtcgaacgcc
     4261 gccgtcacca ccgcgacagg cgcgtcggcg ccggtcctgg tcttggcgtc cacgtatcgc
     4321 tcgaccgaca ggcccgagag cgcgccgagc gcggcgagca cggacgacga ctcgacctcc
     4381 ttggcgggct ggacctgccg ccacgtcgcg gcgccgtcct tgtccgtcgt ccgttcgaac
     4441 gcgactgtgg acgcgccgcg cgtcacctcg aggcgggtgg cggtgaagga gcggaagtcg
     4501 aaaacgtccc tcgggcggta gtcgtccgcc ttcttcttca ggtcgtcgag gatggccggt
     4561 tccaccgtga ccaccaggcg cttcgacgcg tcgcgcgcgt aacggctgcc g
//
LOCUS       DAOSIF010000230         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16585_length_4602_cov_3.480317, whole genome shotgun sequence.
ACCESSION   DAOSIF010000230 DAOSIF010000000
VERSION     DAOSIF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16585_length_4602_cov_3.480317"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLT35_08445"
     CDS             complement(<1..309)
                     /locus_tag="PLT35_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29295.1"
                     /translation="MQRPIVVILARGLGTRMRRGDPGAALADEQARVADTGVKAMIPI
                     GRPFLDYVLSGLADAGLREVCLVIGPEHGAIREYYGTTARPSRLHIEYAIQARPLGTA
                     D"
     gene            complement(288..>4602)
                     /locus_tag="PLT35_08450"
     CDS             complement(288..>4602)
                     /locus_tag="PLT35_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOG29296.1"
                     /translation="VRRGIVDTGLLDRLTVGEEALSERHAKIALLQAAVEAYDAEHNE
                     EVAEFFASAARLRPSVRAEIGRPVELSYRGHRYALRVHRLGEGRARVAAGASRVDVEV
                     EHSGAFERWLTTDGRRYRVLASQSGYTLLIEVEGVPHAIFRADEGIVRASSPAVVVSV
                     HVQPGDEVAAGERLAVLEAMKMETAVAAPFAGTVRQVFVLPNSQVGAGAPLVHLDAAR
                     DERGRKAGPRVVFDGRSHAAARPGGRQASRGRALLHGVRAGLAEYRKAGSERGVVNPL
                     PAMRAVMLGFDLDAKDVERLLQDYDRLTRTLQPDDPELLRAEDNLLRMAVDLRAVFGR
                     QAAAGAESGPSLGSEQHLLRYLRTIDAEGADLPTGFLEELQAALAHYGVTDLRPSRQL
                     RESLLALFQANQRGALHASIVRAVLERRLAAAPRLAPGTPAFLRTIERLIASVQAREA
                     GLADLARDVHYRYVEQPAFERVRGAVLGEMLERLDRLALDPASPEASAWLGDLVACPY
                     PLRTAVTARMATAVPAVRRAALEVMLRRDYRFRRLERLTCVPESDPPLVLAAYPLEGE
                     SIEVVAICGPVDAMASMLLAVREHVASLPADREVIAEIYVWNAQGLDDDAMAAALGET
                     LEAANLARPLRRAVVMVLPRGEPEACGGLKCFTFRPSGPAYREDRVLRGLHPMQAKQL
                     EFSRLERFFLERLPSVEDVYLFKGTARENPRDERLFALVEVRDAAALRDAEGRLAAVP
                     HLERMALEAFTAMRGAQLRRRGEDRLHWNRVTLFVRPPMAVSRPEIGELAARLSTHAE
                     GLGLEKIVIRAALPGADGGVEDVSVGIILAAGRAPVLQFAAPSRDPLRTLSDYDQRVV
                     RMRQRGLTYPYEIVRLMTPEHAEPGDPIPPGSFVELDLDASGALAPVDRPHGKNTANI
                     VVGLLTNRTEKYPEGMTRVALFGDPSREVGSLAEPECRRIIGALDLAEETGVPLEWYA
                     LSAGAKISMESGTENMDWISRVLRRIVTFTQAGGEINIVVTGINVGAQPYWNAEATML
                     MHTRGILVMTAEGAMVLTGKMALDYSGSVSAEDNTGIGGYEHIMGPNGQAQYWARDLR
                     DACRILLRHYDHSYRMPGERFPRQAVTTDPVSRDIRAFPHDGRPDGFATVGEVFSDET
                     NPGRKRPFDIRRVMLAVADADRAPLERWAGWRDAEIAVTWDAHIGGYPVCLIGFESRA
                     VPRVGVVPADGPAQWTSGTLFPMASKKVARAINAASLNRPVVILANLSGFDGSPESMR
                     RRQLEFGAEIGRAVVNFQGPIVFVVVSRYHGGAFVVFSRALNENLEAAALEGSFASVI
                     GGAPAAAVVFARDVDGRTRKDPRVREIDAALASAATDEERAALRASLADVMRKVRSEK
                     LGEVADEFDRIHSVHRALEVGSLDRIISAAELRPYLVGAIERGIARELERWTACSARS
                     S"
BASE COUNT          661 a         1682 c         1609 g          650 t
ORIGIN      
        1 gtcggccgtg ccgagcggcc gcgcctggat cgcgtactcg atgtgcaggc gggagggccg
       61 ggccgtcgtc ccgtagtact cccggatggc gccgtgctcg ggcccgatga cgaggcacac
      121 ctcccggagc ccggcgtcag cgaggccgct caggacgtag tcgagaaacg ggcggccgat
      181 tgggatcatc gccttcacgc ccgtgtccgc cactcgggcc tgttcgtccg cgagcgccgc
      241 gccggggtcg ccgcgccgca tgcgcgtgcc gaggccgcgg gccagaatca cgacgatcgg
      301 gcgctgcatg ccgtccaccg ctccagttcg cgtgcgatcc cgcgttcgat ggcgccgacc
      361 aggtacggac ggagttcggc cgcgctgatg atgcggtcga gcgagcccac ctcgagcgcg
      421 cggtgcacgc tgtgaatgcg gtcgaactcg tcggccacct cgccgagctt ctccgagcgg
      481 acctttcgca tgacgtcggc gagcgacgcg cgcaacgcgg ctcgctcctc gtcagtcgcc
      541 gccgatgcga gggccgcgtc gatctcccgg acgcgcgggt ccttccgcgt gcgcccgtcc
      601 acgtcccggg cgaacacgac ggcggctgcc ggcgccccgc cgatgaccga cgcgaagctg
      661 ccctcgagcg ccgcagcctc gaggttctcg ttcagcgcgc gcgagaagac cacgaacgcg
      721 ccgccgtgat agcgggagac gacgacgaac acgatcgggc cctggaagtt caccacggcc
      781 cgcccgatct cggccccgaa ctccagttgc cgccgccgca tcgattccgg cgagccatcg
      841 aagccggaga ggttggccag aatcaccacc ggccggttca ggctggccgc attgatcgcg
      901 cgcgccacct tcttcgaagc catcggaaac agcgtcccgg acgtccactg cgccgggccg
      961 tcggccggca cgacgccgac ccgcgggacg gccctggact cgaacccgat caggcacacc
     1021 gggtagccgc cgatgtgggc gtcccacgtc acggcgattt cggcgtcgcg ccagcccgcc
     1081 caccgttcga gcggcgcgcg gtccgcgtcg gcaaccgcca gcatcactcg ccggatgtcg
     1141 aagggccgct tgcggcctgg gttggtctcg tcagaaaaca cctcgccgac ggtcgcaaaa
     1201 ccgtcggggc ggccgtcgtg cgggaacgcc cggatgtcgc gcgagacggg gtccgtcgtc
     1261 acggcctgcc gcggaaaccg ctcgccgggc atccggtacg agtggtcgta gtgccgcagg
     1321 aggatccggc acgcgtccct gaggtcccgg gcccagtact gcgcctggcc gttcggcccc
     1381 atgatgtgct cgtagccgcc gatcccggta ttgtcctcgg cggacaccga ccccgagtag
     1441 tcgagcgcca tcttgccggt cagcaccatc gcgccttcgg cggtcatgac caggatgccg
     1501 cgggtgtgca tcagcatggt agcctcggcg ttccagtacg gctgggcgcc gacgttgatt
     1561 ccggtcacga cgatgttgat ttcgcccccg gcttgcgtga aggtgacaat ccggcgcagc
     1621 acgcgcgaga tccagtccat gttctcggtg ccgctctcca tggagatctt ggcgcctgcc
     1681 gacagcgcgt accactcgag cggcacaccg gtctcctccg cgaggtccag cgcgccgatg
     1741 atgcggcggc actccggttc ggccagcgag cccacttccc ggctcggatc gccgaagagc
     1801 gccacgcggg tcatgccctc ggggtacttc tccgtccggt tcgtcaggag ccccacgacg
     1861 atgttcgcgg tgttcttccc gtgcggccgg tcgaccggcg cgagcgcgcc ggaggcgtcg
     1921 aggtcgagct cgacgaacga gcccggcggg atggggtctc ccggctccgc atgctccggc
     1981 gtcatcaggc ggacgatctc gtacggatag gtgagcccgc gctggcgcat ccggacgact
     2041 cgttgatcgt agtcggacag cgtccgcagc gggtcccgcg aaggcgctgc gaactgcaga
     2101 accggcgccc ggcccgcggc gaggatgatg ccgacgctca cgtcctcgac gcctccgtcc
     2161 gcaccgggca aggccgcccg gatgacgatc ttctcgaggc cgagtccctc ggcatgggtc
     2221 gacaaccgcg ccgccagttc gccgatttcc gggcggctca ccgccatcgg ggggcgcacg
     2281 aacagcgtca cgcggttcca gtggagccgg tcttctccgc gacgccgcaa ctgggccccc
     2341 cgcatggccg tgaaggcctc gagggccatt cgctcaaggt gcggcaccgc cgcgaggcgg
     2401 ccttcggcgt ccctgagcgc ggcagcgtcg cggacttcga ccagggcgaa gaggcgctcg
     2461 tccctggggt tctcccgcgc ggtccctttg aagaggtaga cgtcctcaac cgacggcagc
     2521 cgctctagga agaaccgctc gaggcgcgag aactcgagtt gcttcgcctg catcggatga
     2581 aggccgcgca gcacccgatc ttcgcggtag gccgggccgg acgggcggaa cgtgaagcac
     2641 ttcaggcctc cacacgcctc aggctcgccc ctgggcagca ccatgacgac cgcgcgccgc
     2701 agcggacggg cgaggttcgc ggcctccagc gtctctccca gcgcggctgc catggcgtcg
     2761 tcgtcgagtc cctgcgcgtt ccagacatag atctcggcga tcacctcccg gtctgccggc
     2821 aggctggcca cgtgctccct gacggccagg agcattgacg ccatggcgtc gacgggcccg
     2881 cagatcgcca cgacttcaat ggactcgcct tcgagaggat acgccgcgag cacgagcggc
     2941 gggtcggatt ccggcacgca cgtgaggcgc tccaggcggc ggaaccggta gtcgcggcgg
     3001 agcatgacct ccagcgcagc ccgccggacg gcaggcaccg ccgtcgccat ccgcgcagtc
     3061 acagcggtgc gcagggggta cgggcacgcg acgaggtcgc cgagccaggc cgacgcctcc
     3121 ggggacgctg ggtcgagcgc cagccgatcc aggcgctcga gcatctctcc caggaccgcc
     3181 ccgcggaccc gttcgaaggc cggttgttcg acgtagcggt agtggacgtc gcgggccagg
     3241 tcggcgaggc ccgcctctcg agcctggacc gaggcgatga ggcgttcgat cgtgcgcagg
     3301 aacgccggcg ttcccggtgc gagcctgggg gctgcggcaa gccggcgttc gaggacggcc
     3361 cgcacgatcg aggcgtgcag agcgccgcgc tggttcgcct ggaacagcgc cagcaggctc
     3421 tcgcgaagct gcctcgacgg ccgcaggtcc gtgacgccgt aatgcgccag ggcggcctgg
     3481 agctcctcga ggaagcccgt cggcaggtcg gcgccctcgg cgtcgatcgt ccggagatag
     3541 cgcagcaggt gctgctcgga gcccagcgac ggcccggatt ccgcgcctgc ggctgcctgg
     3601 cgcccgaaca ccgccctgag atcgacggcc atgcgcagca ggttgtcctc ggcgcgcagc
     3661 agttcggggt cgtcgggctg aagggtccgg gtgaggcgat cgtagtcctg aagcagccgc
     3721 tcgacgtcct tggcgtccag atcgaagccc agcatgacgg cgcgcatcgc cggcagcggg
     3781 ttcacgacgc cccgctccga gccggccttc cggtactccg caaggccggc gcgcacgccg
     3841 tggagcaagg cccgcccccg agacgcctgc cggccgcccg ggcgcgcggc ggcgtggctc
     3901 ctcccgtcga agacgactct cggcccggct tttcggccgc gctcgtcgcg ggctgcgtcc
     3961 aggtgcacga gaggggcgcc cgcgcccacc tgcgaattgg gaagcacgaa cacctgccgg
     4021 accgtcccgg cgaagggcgc ggccaccgcg gtctccatct tcatcgcctc gagcacagcg
     4081 aggcgctctc cggctgccac ctcgtcgccc ggctggacgt gcaccgacac caccacggcc
     4141 ggcgacgacg cccggacgat gccttcgtcg gcgcggaata tcgcgtgggg cacgccttcg
     4201 acctcgatga gcagcgtgta gccgctctgc gaagccagca cgcggtaccg tcggccgtcg
     4261 gtcgtcagcc agcgctcgaa ggcaccgctg tgctccacct cgacgtccac gcgtgaggcg
     4321 ccggcggcca cgcgggcgcg cccctcgccg aggcggtgca ccctgagcgc gtaccggtgg
     4381 ccgcggtagc tcaactcgac cgggcgcccg atctccgccc ggacgctggg gcgaagccgc
     4441 gcggccgagg cgaagaactc cgcgacctct tcgttgtgct cggcgtcgta cgcctcgacg
     4501 gcggcctgca gcagcgcgat tttcgcgtgg cgttccgaga gggcctcctc gccgaccgtg
     4561 aggcgatcca gaagccctgt gtcgacgatg ccccgccgca cc
//
LOCUS       DAOSIF010000231         4574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16701_length_4574_cov_3.637309, whole genome shotgun sequence.
ACCESSION   DAOSIF010000231 DAOSIF010000000
VERSION     DAOSIF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16701_length_4574_cov_3.637309"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..521)
                     /locus_tag="PLT35_08455"
     CDS             complement(180..521)
                     /locus_tag="PLT35_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG29297.1"
                     /translation="MTRRSLVSLVGGLALLAAAAVPSFAGSIQAGAPAKHAKPAATAA
                     AKPAELLDLNTATKEQLAALPGIGEAYAQKIIEGRPYARNSELKTRNILPEAVYAKVA
                     KLVVARHHVKK"
     gene            847..2403
                     /gene="rmuC"
                     /locus_tag="PLT35_08460"
     CDS             847..2403
                     /gene="rmuC"
                     /locus_tag="PLT35_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOG29298.1"
                     /translation="MTVAVDVLVAAFAGLLVGAAISWLAGALRVRSRERQAAALAAAE
                     AEARAGQALAASMAARARLEATLEAEREASAEAARALEGRIAELQRASAALAHDLKAS
                     ADALLEVRSRESRLSAELAKDREAHEERLAAYRDAEQRLKEAFAAVSADTLDANARRF
                     LELASARLEAIASGAQQDLAERQARISSALEPVKAALGKVESTLASVESARQQERGQT
                     SAQLASLGETQRQLEAETRRLVQALRAPQVRGRWGEVQLRRVVELAGMEQYCDFEVQP
                     TIDGDQLVLRPDLIVTVPGGRSIVVDAKAPVMAYLEAVDASEADVREARLRDHAGQVR
                     AHVSKLGGKGYWEHLERTPDFVVLFLPGEAFFSAALQHDPGLFEYGVERRVFLAGPMT
                     LLALLRTVAYGWTQEKLAANAQEISALGRDLYERLCTMFDHLGDLQRKLDSAVQAHNA
                     AVASLESRVLPAARRFKDLGVRSAREIEGPEPVERQARLLQAVEAATARNTAAVDATV
                     VGRGAEQAPD"
     gene            complement(2470..3273)
                     /locus_tag="PLT35_08465"
     CDS             complement(2470..3273)
                     /locus_tag="PLT35_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29299.1"
                     /translation="MKSRWWVWVAAVALTLGSAVYQRLTGPTNPARGTVTIGGREHRL
                     RLTRTHPGAGDQPVVLTVPDRDVGGRVAWRRYPTTDAWQVLPLERDGDTLRTALPHQP
                     VAGKLEYQVLLERGSERAVFPGRPAITRFRQDVPAGVLIPHVAAMFLAMLFSTAAGLG
                     ALARHKQARRDARICMVLMFLGGFVLGPLMQYYAFGEWWTGVPFGWDLTDNKTLMAFA
                     AWLWAAWRMRRGGEARTAIVLASVATLIVFAIPHSTWGSEYKWEAAQGS"
     gene            3516..>4574
                     /gene="metK"
                     /locus_tag="PLT35_08470"
     CDS             3516..>4574
                     /gene="metK"
                     /locus_tag="PLT35_08470"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG29300.1"
                     /translation="MEHLPYTVSSESVSEGHPDKVCDLIADTVLDKYLEQDPDSHVAC
                     EVMCKNNLVVLGGEISSKGRVPYEDIVRETIRGIGYVDPAEAFNADGVQVLIAISRQS
                     AEIDQGVSRAVAAEQGAGDQGMMYGYATDETPELMPLPVLLAHRLTRGLADDRRAGTE
                     RCAWLRPDAKSQVSVVYENGAPVRVSHVLVSTQHRQGAARDTIAEYVRHTLAPRVLGS
                     WFTKDVEVLVNPTGSFVLGGPSADCGVTGRKIIVDTYGGAGRHGGGAFSGKDPSKVDR
                     SGAYFCRFVARQIVKQGLAKRAEIQVAYAIGMAQPVSIKVDTFGTGDDRAAAALVKTF
                     DFRPRPIIDRLGLLRPIYR"
BASE COUNT          682 a         1592 c         1666 g          634 t
ORIGIN      
        1 ccgatcctta cgcgcccgcg tcccggtcag cccattctac gccggccgcc ggcaggccgc
       61 cggatgagcc gctccggggg ccgcctggat ccgggccgcg cgaggagcgg ggaggcgaac
      121 gccccggcgg gcgggggagc ggccggcgtc ggcacgccgg ggcgttcgca tcagggcggc
      181 tacttcttga cgtggtgtcg ggcgacgacg agcttggcga ctttcgcgta cacggcctcc
      241 ggcaggatgt tcctggtctt cagctcggag ttcctggcgt agggccggcc ctcgatgatc
      301 ttctgggcgt acgcctcacc aatgcccgga agggcagcca gctgctcctt cgtcgcggtg
      361 ttgagatcca gcagttcagc tggcttcgcg gctgctgtcg ccgccggctt ggcgtgcttg
      421 gcgggggcac ccgcctggat ggagcccgcg aacgacggca ccgcggccgc cgcgagaagc
      481 gcaaggcccc cgacgagcga tacgagagag cgacgcgtca tacgagtatt cctaccttcc
      541 tgcgcacccg gccacaagag gcgcccgacc gtcatgatcg gcaccgtgcc ccgtccgcct
      601 tccgggtgat cgcgcggaca ccaggactgc aacaagaagc gtgccggtgc gtcgatgccg
      661 gaatgcgcgg caatccggcg gcacgtgacg aaatcgggtt cctggcggct tcgacaggcg
      721 gtgcgttctg ccccaggcgg ggcaatcggc cacaccggac tgctcggcgc ggcggcccgc
      781 gtctggggcg cccgccacgg cgggcgcacg cttgaccccg cgtccgggcc gggccatcat
      841 cgggccatga cggttgccgt cgatgtcctc gtggcggcct tcgccggcct gcttgtcggc
      901 gcggcaatca gctggctggc cggcgcgctg cgtgtccgtt cgcgcgaacg ccaggcggcg
      961 gccctcgctg cggccgaggc ggaggctcgc gcagggcagg ccctggccgc gtcgatggcg
     1021 gcccgcgcgc gcctggaggc gacgctggag gcggagcgcg aggcgtcggc cgaggcggcc
     1081 cgcgcgctcg aaggccgcat cgccgagctc cagcgagcga gcgcggccct cgcccacgat
     1141 ctgaaggcgt cggcggacgc gctgctggag gtccggagcc gggagagccg gctcagcgcg
     1201 gagcttgcca aggaccgcga ggcccacgag gaacgcctgg ccgcctaccg ggacgccgag
     1261 cagcgtctga aggaggcgtt tgccgccgtt tccgccgaca cgctcgacgc caacgcgcgg
     1321 cgtttcctcg agctggcgtc agccagactg gaggcgattg ccagcggcgc gcagcaggat
     1381 ctcgccgagc gccaggcgcg catctcttcg gcgctcgagc cggtcaaggc cgccctggga
     1441 aaagtcgagt ccacgctggc atccgtcgaa tcggcgcgac agcaggagcg gggccagacg
     1501 tcggcccaac tggcgtcgct cggcgaaacg cagcggcagc tcgaggccga aacccgcagg
     1561 ctcgtgcagg cgctgcgcgc gccgcaggtc cgcggccgat ggggcgaggt ccagttgcgc
     1621 cgcgtggttg aactggccgg gatggaacag tactgcgact tcgaagtgca gccgacgatc
     1681 gacggcgacc agctggtgct ccgccccgac ctcattgtca cggtgcccgg cgggcgcagc
     1741 atcgttgtgg acgcgaaggc ccccgtcatg gcgtatctcg aggccgtcga cgcctcggag
     1801 gccgacgtgc gcgaggcccg gctgcgcgac cacgccggcc aggtgcgggc tcacgtgagc
     1861 aagctgggcg gcaagggcta ctgggagcac ctggagcgga cgccggattt cgtggtgctg
     1921 ttcctgccgg gggaggcgtt cttcagcgcc gcgctccagc acgatccggg gctgttcgag
     1981 tacggcgtgg agcggcgcgt gttcctggct ggcccgatga cgcttctcgc cctgctgcgg
     2041 acggtcgcct acgggtggac gcaggagaag ctggcggcga acgcgcagga aatcagcgcc
     2101 ctggggcggg acctgtacga gcgcctgtgc acgatgttcg atcacctcgg cgacctgcag
     2161 cgaaagctcg attccgccgt gcaggcgcac aacgcagccg tggcgtccct cgagagccgg
     2221 gtgttgccgg ctgccaggcg gttcaaggac ctcggcgtcc ggtctgcgag ggagatcgag
     2281 ggcccagagc cggtggaacg ccaggcgcgg ctccttcagg ccgttgaggc cgccactgcg
     2341 cgcaacacgg cggcggtcga cgcgaccgtc gttggccgcg gcgcggagca ggccccggac
     2401 taggctcggc gccgcctcat ctggcgcagg gagcggcgcc ggcgccgggt cgtcatgcag
     2461 ccggcgcgct caggaaccct gcgccgcctc ccacttgtat tcggagcccc atgtgctgtg
     2521 cgggatggca aacacgatga gcgtcgccac cgaggcgagg acgatcgccg ttcgggcctc
     2581 gccgccgcgg cgcatccgcc aggcggccca cagccaggcc gcgaacgcca ttagcgtctt
     2641 gttgtccgtc agatcccacc caaacggcac gccggtccac cattcgccaa aggcgtagta
     2701 ctgcatcaag gggccgagga cgaacccgcc caggaacatc aggaccatgc agatgcgggc
     2761 gtcgcgcctg gcctgcttgt gccgggcgag cgcgccaagg cctgccgccg tcgagaagag
     2821 catcgcgagg aacatggcgg ccacgtgggg gatcagcacg ccggcgggca cgtcctgccg
     2881 gaatcgcgtg atggcgggac gcccggggaa caccgcccgc tcgctgccgc gctcgagcag
     2941 cacctgatac tccagcttgc cggcaaccgg ctggtggggg agggccgtcc gaagggtgtc
     3001 gccgtctcgc tccagcggca ggacctgcca ggcgtcggtg gtgggatagc gcctccaggc
     3061 gacgcggccg ccgacgtccc gatcgggcac cgtcagcacc acgggctggt ccccggcgcc
     3121 ggggtgggtc cgcgtgaggc gcagccggtg ctcccggccg ccgatcgtca ccgtgcctcg
     3181 agccgggttc gttgggcctg tcagccgctg atagacggcc gagcccagcg tcagcgcgac
     3241 tgccgccacc cacacccacc aacggctctt catgcaatgc ctccgacggc gcgggccgcc
     3301 ccgagcccgc gccgtctcat gataacaaac ggcgcgcttg acatcccgcg ggcgcgccag
     3361 ccatagtggg gcgataggaa ggcggcgatt tccccgactt gatgggccgc gccaggccgg
     3421 tctcgaaccg cccctggaac tatctaagtc gcggctgggc tcgcgacggc acgtcggcga
     3481 gcctttgctg tttctggaag aaaggccagg agaccatgga gcatctgccg tacaccgtgt
     3541 cctcggagtc cgtctccgaa gggcatcccg acaaggtctg cgacctcatc gccgacacgg
     3601 tcctcgacaa gtacctcgag caggatccgg acagccacgt cgcctgcgag gtgatgtgca
     3661 agaacaatct cgtcgtcctc ggcggcgaga tcagctcaaa ggggcgcgtt ccatacgagg
     3721 acatcgtcag ggagacgatc cgcgggatcg gctacgtcga tccggcggag gcgttcaacg
     3781 cggacggcgt ccaggtgctc atcgcaatca gccgccagtc ggccgagatc gaccagggcg
     3841 tgagccgcgc cgtggccgcg gagcagggcg ccggcgatca aggcatgatg tacggctacg
     3901 cgacggacga aaccccggaa ctgatgccgc tgccggtcct cctggcgcac cggctcacgc
     3961 gcggcctcgc cgacgaccgc cgggccggca cggagcgctg cgcctggctg cggcccgacg
     4021 ccaagtcgca ggtgtcggtc gtgtacgaga acggcgcgcc ggtccgcgtg tcgcacgtgc
     4081 tggtgtcgac gcagcaccga cagggcgcgg cgcgggacac catcgcggag tacgtccggc
     4141 acacgctggc gccgcgcgtg ctcggttcct ggttcaccaa ggacgtcgag gtcctcgtca
     4201 accccacggg gagcttcgtc ctcggcggcc cgtcggccga ctgcggcgtc accggccgca
     4261 agatcatcgt ggacacctac ggcggcgccg ggcggcacgg cggcggcgcc ttcagcggca
     4321 aggacccgtc gaaggtggac cggtcaggcg cgtacttctg ccgcttcgtc gcgcggcaaa
     4381 tcgtcaagca ggggcttgcc aagcgggccg agattcaggt ggcgtacgcc atcggcatgg
     4441 cgcagccggt ctcgatcaaa gtggacacct tcggcaccgg cgacgatcgg gcggcggccg
     4501 cgctggtgaa gacctttgac ttccggccac ggccgatcat cgatcgcctc ggcctgcttc
     4561 ggccgatcta ccgc
//
LOCUS       DAOSIF010000232         4536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16881_length_4536_cov_3.192591, whole genome shotgun sequence.
ACCESSION   DAOSIF010000232 DAOSIF010000000
VERSION     DAOSIF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16881_length_4536_cov_3.192591"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLT35_08475"
     CDS             complement(<1..523)
                     /locus_tag="PLT35_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOG29301.1"
                     /translation="MNRWLVAITAGVIGCLLAGAAPASAQGFGVFEQGSCAMGRGGAG
                     VAAPCPDASGIYFNPAGLSLDRTQISLGAALIGPKGDFTNSTTSLVSQLKKAWYPVPN
                     VYASTPISDKVAAGIGLFAPYGLTSEWPLTAEGRFLGYKSVVQAVYVQPTLAFKASDR
                     LSIGVGVDVAYVKL"
     gene            676..852
                     /locus_tag="PLT35_08480"
     CDS             676..852
                     /locus_tag="PLT35_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29302.1"
                     /translation="MQCERCGAGPSGAELFDYCGLCGQTLCPYCMAAGCCGQTPAVSG
                     RTEDLDPDDEDRER"
     gene            complement(890..2998)
                     /locus_tag="PLT35_08485"
     CDS             complement(890..2998)
                     /locus_tag="PLT35_08485"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG29303.1"
                     /translation="MNRRRFIVPLLALAVALGAAPRADEGFWPYNSVPKAAIKAKYKF
                     DVTDAWLKHLQLATVRFGGGTGSIVSPRGLVLTNHHIGLGTLQRLSTPEKDYVKNGFY
                     APTFADELKVPGMSLMVLQTIEDVTAKVADAVTPSMTAQEAAAARQKAIQAIQGEPAQ
                     GVSKQVVSLYAGAVYNLYTYKVYSDVRLVFGAEYQTGFYGGDADNFTYPRYNLDVSMF
                     RLYENDKPAETPNYLRWSPNGSKENELVFTTGHPGSTQRLNTVAHLKYLRDLTVPFTI
                     ASAEMRETAVKKWMAQSPENARQATSELFGLQNSLKSYRGRLKGLQDPSVIARKEAAE
                     ARLRAELAKNAAKQQLLGDAWDLVEKSIGVARQIDAERNFIVNAAGLNSTLFAQARQI
                     VRRAYAPQTADGGRGGRGAGPGGPGGPGGPGGPGGPGGPGLTQGAPNLAREKLNLTES
                     LAFMQKHLGPDHAIVKRILAGKAPEARAAELVDGTRITDSEIRALLVAGGKATIDVST
                     DPMIKLARDLEPDAQAIAKRYEDEVAGVQAIAYPKIGQAVFAVDGPKAYPDATGTARI
                     SYGVVKGYLEDGKKIPPYTYVPGLYERAALHAGQPDYALAPAWVAAKAALNPKTPFNF
                     VSTNDIVGGNSGSAVVNKKGELVGLIFDGNIQSLPGYYIFDEPNRAVSVDSRIIIEAF
                     RKVYNAGALADEITGAAARK"
     gene            complement(3099..4115)
                     /locus_tag="PLT35_08490"
     CDS             complement(3099..4115)
                     /locus_tag="PLT35_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG29304.1"
                     /translation="MKRLRILALVHKHLVPPEDTAGVDTVNAEWKMEFDVTVTLEAQG
                     HEVRIAGVHDDLSPARAAIDEFRPDIVFNLLEGFADISTFDQNVVSYLELLRVPYTGC
                     NPRGLILARDKSLSKKLLTYHRVPVPDFTVVRRGRSARPTRLRYPLIVKSLVFEASIG
                     ISQASVVENADELARRIQFVHESLGTAAIVEEFIDGRELYVGVIGNERLQAFPVWEMS
                     FAQMPEHTWHVATERVKWSTKYQQKHGIRTDEASLPEGVAARLQHTAKRVYRALDLNG
                     YARVDFRMDQAGRFYVLEANPNPQLAFGEDFAESAERAGLSYPALLERIVALGLRWRP
                     ERVQ"
     gene            complement(4112..>4536)
                     /locus_tag="PLT35_08495"
     CDS             complement(4112..>4536)
                     /locus_tag="PLT35_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29305.1"
                     /translation="SLAGRPPCVTRADEMDALRRLRITLRTHYRRKRRHYGVDRPDFY
                     DRDLRRLFSDAPEFATSPTAAAFVGRIRRQVRRLVADWTGIYQYTIDQVLEDIIVRCR
                     ELKLRLAVPEDTARQEFTVLLTVQAMNFLHSGRHRLAL"
BASE COUNT          627 a         1564 c         1585 g          760 t
ORIGIN      
        1 gcagcttgac gtaggccacg tccacgccga cgccgatcga cagccgatcg gaggccttga
       61 acgccagcgt cggctgcacg tagaccgcct ggacgacgct cttgtagccc aggaagcggc
      121 cttcggcggt gagcggccac tccgaggtca ggccgtacgg ggcgaacagg ccgatgcccg
      181 ccgcgacctt gtcggagatc ggcgtcgacg cgtacacgtt gggcaccggg taccaggcct
      241 tcttcagctg gctgacgagg ctggtggtgc tgttggtgaa gtcgcccttg gggccgatga
      301 gcgccgcgcc gagggagatc tgggtccgat cgagcgacaa gccggccggg ttgaagtaaa
      361 tcccgctggc gtccgggcac ggagcggcga cgccggcgcc gcctcgcccc atcgcgcagg
      421 agccctgctc gaacacgccg aacccctgcg cggaggccgg tgcggcccct gccagcaagc
      481 agccgatcac gcctgcagtg atggcgacca accatcggtt catagacact gcctcccagt
      541 agtggacgga tagtccgcac agcctatcac gggtcgtctc cgaaccgtag gggattcccc
      601 ggccccgcgc ttgcggtccc ggccggggcg gtctatagtc gggccggggt cggtctaacc
      661 cgaagggagg tgcccatgca atgcgaacga tgcggggccg ggccgtccgg cgccgagctg
      721 ttcgactact gcgggctctg cgggcagaca ctgtgcccgt actgcatggc cgccgggtgt
      781 tgcggccaga ctcccgcggt gtcgggccgg accgaggacc tcgatccgga cgacgaggac
      841 cgcgagcggt gagggaatcg gggccgggct tgcgcccggc cccgtcgagc tacttcctgg
      901 ccgccgcccc cgtgatctcg tcggccagcg ccccggcgtt gtacaccttc cggaacgcct
      961 cgatgatgat ccgggaatcc acggacacgg cccggttggg ctcgtcgaag atgtagtacc
     1021 ccggcagcga ctggatgttg ccgtcgaaga tcaggcccac cagttcgccc ttcttgttga
     1081 ccaccgccga cccgctgttg ccgccgacga tgtcgttcgt gctgacgaag ttgaagggcg
     1141 tcttcgggtt gagcgccgcc ttcgccgcca cccacgccgg cgccagggcg tagtccggct
     1201 gccccgcgtg cagggccgcg cgctcgtaga gcccggggac gtacgtgtag ggcgggatct
     1261 tcttgccgtc ctccaggtac cccttcacca cgccgtagga aatccgcgcc gtgccggtcg
     1321 cgtcgggata cgccttcggg ccgtccacgg cgaacaccgc ctgcccgatc ttcggatagg
     1381 cgatggcttg gacgccggcg acctcgtcct cgtaccgctt ggcgatcgcc tgggcgtccg
     1441 gctcgaggtc ccgcgccagc ttgatcatcg ggtcggtgga gacgtcgatg gtcgccttgc
     1501 cgccggccac gagcagcgcc cggatctcgg aatcggtgat ccgcgtgccg tccaccagtt
     1561 ccgcggccct ggcctccggg gccttcccgg ccaggatccg cttgacgatg gcgtggtctg
     1621 gcccgaggtg cttctgcatg aaggcgagcg actcggtcag gttcagcttc tcgcgtgcga
     1681 ggttcggggc cccctgcgtc aggcccggcc cgcccggccc gcctggcccg cctggtccgc
     1741 caggtccgcc gggtccgccc ggtccggccc cgcgcccgcc gcggccgccg tcggctgtct
     1801 ggggtgcgta ggcgcggcgg acgatctggc gcgcttgggc gaacagcgtc gagttcaagc
     1861 cggccgcgtt cacgatgaag ttccgctcgg cgtcgatttg gcgcgcgacc ccgatggact
     1921 tctcgacgag gtcccaggcg tcgcccagca gctgctgctt ggcggcgttc ttcgccagtt
     1981 cggcgcggag gcgcgcctcg gcggcttcct tcctcgcgat gacggacggg tcctggaggc
     2041 ccttcagccg cccgcggtac gacttcaggc tgttctggag gccgaacagc tcgctggtcg
     2101 cctggcgcgc gttctcgggg ctctgggcca tccacttctt caccgccgtc tcccgcatct
     2161 cggcgctggc gatggtgaaa ggcacggtca ggtccctgag gtacttgagg tgcgccacgg
     2221 tattcaggcg ctgcgtcgag ccggggtggc cggtcgtgaa caccagctcg ttctccttcg
     2281 agccgttggg cgaccacctc agatagttcg gcgtctcggc cggcttgtcg ttctcgtaca
     2341 ggcggaacat cgagacgtcc aggttgtagc gcggataggt gaagttgtcg gcgtcgccgc
     2401 cgtagaagcc ggtctggtac tcggcgccga agaccaggcg gacgtcgctg tagaccttgt
     2461 acgtgtacag gttgtagacg gcgcccgcgt acagcgacac gacctgcttc gagacgccct
     2521 gtgcgggctc gccctggatc gcctggatcg ccttctgccg cgcggccgcg gcttcctggg
     2581 cggtcatcga cggcgtgacg gcgtcggcca ccttcgcggt cacgtcctcg atggtctgga
     2641 gcaccatgag cgacatgccc gggaccttca gctcgtcggc gaaggtcggc gcgtagaagc
     2701 cgttcttcac gtagtctttc tcgggcgtgc tgaggcgctg gagcgtgccg aggccgatgt
     2761 ggtggtttgt cagcacgagg ccccgcgggg agacgatcga cccggtgccg ccgccgaagc
     2821 gcaccgtcgc aagctgcagg tgcttcagcc acgcgtcggt cacgtcgaac ttgtacttcg
     2881 ccttgatggc ggccttcggg acgctgttgt agggccagaa cccctcgtct gcccgcggcg
     2941 cggcgccgag ggcgaccgcc agggcgagga gcgggacgat gaatcgccgt ctgttcatgg
     3001 tgtgacctcg tctggaagag cgaccacccg actctagtga ggcgccgcag ggctgtcaag
     3061 ccggcgtcct ccaggaggcg tcccggggac ggcccggcct actggacgcg ctcgggccgc
     3121 caccgcagcc cgagcgccac gatgcgctcg agcagcgcgg gatacgacag gcccgcccgt
     3181 tccgccgatt ccgcgaagtc ctcgccgaat gccagctgcg ggttcgggtt cgcctcgagg
     3241 acgtagaacc gcccggcctg gtccatccgg aagtcgacgc gcgcgtaccc gttcaggtcg
     3301 agcgcgcggt agacgcgctt ggccgtgtgc tgcaggcgcg ccgcgacgcc ctcgggcagc
     3361 gacgcctcgt cggtcctgat cccgtgcttc tgctggtact tcgtgctcca cttcacccgc
     3421 tccgtggcca cgtgccacgt gtgctcgggc atctgcgcga acgacatctc ccagacgggg
     3481 aaggcctgca ggcgctcgtt gccgatgacg ccgacgtaca gctcgcggcc gtcgatgaac
     3541 tcctcgacga tggcggccgt gccgaggctc tcgtggacga actggatgcg ccgcgcaagc
     3601 tcgtccgcgt tctcgaccac cgacgcctgc gagatgccga tggacgcctc gaacaccagc
     3661 gacttcacga tgagggggta gcgcagccgc gtgggccggg ccgagcggcc gcggcggacc
     3721 accgtgaagt cggggaccgg cactcggtgg tacgtcagca gcttcttgct gagcgacttg
     3781 tcgcgcgcca ggatgagccc gcgcgggttg cagccggtgt agggcacccg cagcagctcg
     3841 aggtagctca ccacgttctg gtcgaaggtg ctgatgtcgg cgaagccctc gagcaggttg
     3901 aacacgatgt cgggcctgaa ttcgtcgatg gccgcccgcg ccggcgacaa gtcgtcgtgg
     3961 acgcccgcga tccgcacctc gtgcccctgc gcctcgaggg tcaccgtgac gtcgaattcc
     4021 atcttccatt cggcgttgac cgtgtccact ccggccgtgt cctccggcgg cacgaggtgc
     4081 ttgtgcacga gcgcgaggat gcggagccgc ttcatagcgc cagacggtgg cggccgctgt
     4141 gcaggaagtt catggcctgc accgtcagca gcaccgtgaa ctcctgccgg gccgtgtctt
     4201 cagggaccgc caggcgcagc ttcagctcgc ggcaccgcac gatgatgtcc tcgagcacct
     4261 ggtcgatcgt gtactgatag atgcccgtcc agtcggccac gagccggcgc acctgccggc
     4321 ggatgcgccc gacgaaggcg gccgccgtcg ggctggtcgc gaactccggc gcgtccgaga
     4381 acaggcgccg gaggtcccgg tcgtagaaat cgggccgatc gaccccgtag tggcgccgct
     4441 tccgtcggta gtgcgtccgc agcgtgatgc gcagccgccg cagcgcgtcc atctcgtctg
     4501 cgcgcgtcac gcagggcggc cgccccgcga gcgacg
//
LOCUS       DAOSIF010000233         4527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_16917_length_4527_cov_3.611136, whole genome shotgun sequence.
ACCESSION   DAOSIF010000233 DAOSIF010000000
VERSION     DAOSIF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16917_length_4527_cov_3.611136"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLT35_08500"
     CDS             <1..449
                     /locus_tag="PLT35_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29306.1"
                     /translation="GAVAAMVSTAESMLIVAGTAVSQDAYKGIIRRGQVSDRALLNVS
                     RGTTLAIGVLSLVLALTTEKLIYSIVSYAWAGIGCSFAPAVLLSFYWDRFCSAGVVTA
                     LLVGLLTTIVWIATGLDSRVTAMAVTFAAAMGSAVLVTLAAPPRRA"
     gene            complement(472..2733)
                     /locus_tag="PLT35_08505"
     CDS             complement(472..2733)
                     /locus_tag="PLT35_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG29307.1"
                     /translation="MTLRTRLSLAFFFISVVPLAVVTAYSYYSSAAALRHAVEAQADQ
                     MASEMGDRLTWVMADLGERVERLWRMRVQEPAALGAPRTAGALPSAVANSETLQHLAA
                     AMLAEAAPLVRRLEFSSAADTAAVPGSAGAPPAQAPAQAESGGGRTGGRGGRARGAWQ
                     APGAPSAGRAPESGPRGPAGRPQPPPGVPVVSPGMPKVVIDMPTARAAQGSANSEALF
                     EALTPEAVNAWRRAIQRQAEMDADQAGIPPRPPAPPPSPEPGQPSASGAPDGQAGAPA
                     FGRAPRAGAAGLSASELRTLRDRRRAERAERMKALATGEALQFDVRRAGQLIGTISAT
                     VDRNRIIETVLSLGRRERGEVPFVMDSSGEIHALDPAGRRAIEALRLAPVAPGRSAST
                     TLAGDVLVVTRRDPSGIVFGLARPLRDSLRDMRRASLGNLAVGLLFIAGAFAMILPLA
                     SRLTRHLAALNAGVQRLAAGDRHARVAEDAPDEAGELARSFNRMAAELEVHDRVLAKQ
                     ERLARELELGRQIQTDMLPRGPLKTSFVEVAGVSIPAREVGGDFFNYFLLEDGRIALL
                     VGDVSGKGVGAALLMANIQATLRARLQLESDLAQLADAVDHDIAANTPPEVYATLFAG
                     VMDPAGSELRYVNAGHNPQFLLRAGGGLQRLESTGLPIGLLPGRGYVERRVGVERGDL
                     LFLYTDGAIEAPNESGEFFDADRLQRELVEASALDVDALLVRIERALRAFRGRAEPAD
                     DATMMALRLSPAR"
     gene            2787..3749
                     /locus_tag="PLT35_08510"
     CDS             2787..3749
                     /locus_tag="PLT35_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOG29308.1"
                     /translation="MPPVCAVGPVEWGIEMRLFLTILADDILPIFLVAAVGFVLARRL
                     RVDVKSVSRVTFNALAPCLVFTQLVTSRVGAGEFGRIVAFTILLVGSAGAVARLAAIP
                     FRLERPALAAFLIVVMFSNSANYGLSVVLFAFGEEALSRAVVYFATGAMLVYSVGSFL
                     ASSGRRSAREAAAGLLRVPALWGLLAAAVVIGTGATLPAFVLRPATLLSSAAIPTMLL
                     VLGMQFERGAWPERPALVATAALLGLVGSPLIGFGLAWLLGLEGVSRQAVLVEAAMPS
                     AVITTILAVEFDVEPRFVTSVVVVTTLASAVTVSILIALLKSGY"
     gene            complement(3685..>4527)
                     /locus_tag="PLT35_08515"
     CDS             complement(3685..>4527)
                     /locus_tag="PLT35_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOG29309.1"
                     /translation="ADACADGLARLAAAIRGEGAVACLQIAHAGSRASSRVTGLPVVS
                     ASPVATGREAHEETPAALDTGGIANVVEAFAAAAARARRAGFDAVEVHAAHGYLLSQF
                     LSPLTNRREDDYGGDAARRARLTADVVTAITQRVGPGFPVFVRLGMHDERPGGLTLAP
                     ACWTAARLVEAGASLIDVSGGLAGSDDPGRGPGYFVPYAEALKDVVNVPVLVAGGITD
                     PRTADVIVRSRRADIVGVGRAMLADPAWAARAVEMLREAPAASTPTSGAQSGSTPSPR
                     WPAS"
BASE COUNT          560 a         1657 c         1656 g          654 t
ORIGIN      
        1 ccggcgcggt cgcggccatg gtgtcgaccg ccgaaagcat gctcatcgtg gcgggaaccg
       61 cggtcagcca ggacgcctac aagggcatca tccgccgggg gcaggtgtcc gatcgcgccc
      121 tgctgaacgt ctcccgcggc acgacgctgg cgatcggcgt gctcagcctc gtgctcgccc
      181 tgacgacgga gaagctcatc tactcgatcg tcagctacgc ctgggccggc atcggttgct
      241 cgttcgcgcc ggcggtgctg ctgtcgttct actgggaccg cttctgctcc gccggcgtcg
      301 tcacggcgct cctcgtgggc ctgctcacga ccatcgtctg gatcgccacc gggctggatt
      361 ctcgcgtcac cgccatggcc gtgacgttcg ccgccgcgat gggatcggcc gtgctcgtga
      421 ccctggcggc gccgccccgc cgggcgtgac cggcgcgcgc cgccatgccc ttcagcgcgc
      481 cgggctgagg cggagcgcca tcatcgtcgc gtcgtcggcg ggctcggcgc ggccgcggaa
      541 tgctcgcagc gcgcgttcga tccgcacgag cagcgcgtcg acgtcgaggg cggacgcctc
      601 caccagctcc cgctgcaggc ggtcggcgtc gaagaactcg cccgattcgt tcggcgcctc
      661 gattgcgccg tccgtgtaga ggaagagcag gtcgccgcgc tcgacgccga cacggcgttc
      721 cacgtagccg cgccccggga gcaggccaat cggcaggccg gtcgattcca ggcgctggag
      781 cccgcctccc gcccggagca ggaactgcgg attgtgcccg gcgttgacgt accggagttc
      841 gctcccggcc gggtccatca cgccggcgaa gagcgtcgca tagacctcgg gcggcgtatt
      901 ggccgcgatg tcgtggtcca cggcgtccgc cagctgagcc aggtccgact cgagctggag
      961 tctcgcccgc agcgtggcct ggatgttcgc catcagcagc gccgcgccca cgcccttgcc
     1021 cgacacgtcc cctaccagca gggcgatgcg cccgtcttcc aggaggaagt aattgaagaa
     1081 gtccccgccc acctcgcggg cgggaatcga cacgccggcg acctcgacga agctggtttt
     1141 cagcggcccg cggggcagca tgtcggtctg gatttgccgg ccgagctcca gctcacgtgc
     1201 gaggcgctcc tgcttcgcga gcacccgatc gtggacttcc agctcagccg ccatccggtt
     1261 gaacgagcgc gccagctctc cggcttcgtc cggggcgtcc tcggcgacac gggcatggcg
     1321 gtcgcccgcc gccagccgct gcacgccggc gttgagcgcg gccagatgcc gcgtgagccg
     1381 gctggccagc ggcaggatca tcgcgaacgc ccccgcgatg aacagcagcc cgacggcgag
     1441 gttgccgagg gaggcgcggc gcatgtcgcg gagcgagtcg cggagggggc gcgcgaggcc
     1501 gaagacaatg cccgaggggt cgcggcgggt gacgacgagc acgtctccgg ccagcgttgt
     1561 cgaggcactc cggcccggcg ccaccggcgc gagcctgagc gcctcaatgg cgcgccgccc
     1621 cgcgggatcg agggcgtgga tctcgccgct cgaatccatg acgaacggga cctcgccccg
     1681 ctcccggcga ccgagggaga gcaccgtctc gatgatgcgg ttccggtcca ccgtggcgct
     1741 gatcgttccg atgagctggc ccgcccgccg gacgtcgaac tgcagggctt cgccggtcgc
     1801 cagggccttc atgcgctccg cgcgctcggc gcgccgccgg tcccgcagcg tgcggagctc
     1861 ggacgccgac agtccggccg ctcccgcgcg aggcgctcgg ccgaacgccg gcgcacccgc
     1921 ctggccgtcg ggtgctccag aggcgctcgg ctgacccggt tcgggggagg gaggcggggc
     1981 agggggcctg ggcgggatgc ctgcctggtc ggcatccatc tcggcctggc gctggatggc
     2041 ccggcgccac gcattgacgg cctccggagt gagcgcctcg aagagcgcct ccgagttggc
     2101 gctgccctgg gccgcacgcg ccgtcggcat gtcaatcacg accttcggca tgccgggcga
     2161 gaccaccggt acaccgggcg gcggctgggg ccggcccgcc ggccctcgcg ggccggactc
     2221 gggcgctcga ccggccgaag gcgcgccagg ggcctgccac gcgccacgag ccctcccgcc
     2281 ccggcctccc gttctcccgc ccccggattc ggcttgggct ggcgcctggg ccgggggggc
     2341 acccgcggaa cctgggacgg ccgcggtgtc ggcagccgac gagaactcga ggcgccggac
     2401 gagcggcgcc gcttcagcga gcattgcggc ggcgaggtgc tgaagggtct ccgagttcgc
     2461 cacggccgag gggagcgcgc ccgccgtcct cggcgcgccg agagccgcgg gctcctgcac
     2521 gcgcatccgc cagagccgct cgacgcgctc cccgagatcc gccatgaccc atgtcaggcg
     2581 atcgcccatc tcggaggcca tctggtccgc ctgcgcttcg acggcgtgcc ggagcgccgc
     2641 agcggacgag tagtacgagt aggccgtgac aaccgcgagc ggcacgaccg agatgaagaa
     2701 aaacgcgagc gacaagcgtg tcctgagtgt catggcgtta ccaggatcct acgcccgcag
     2761 cgggcagccc gtttcacgct cggcgagtgc cgccggtctg cgccgtcggc cctgtagaat
     2821 ggggcatcga gatgcgcctc ttcctgacca tcctggccga cgacatcctg ccgatcttcc
     2881 tggtggcggc tgtgggcttc gtgctggctc ggcgccttcg cgtcgacgtg aagtccgtgt
     2941 cgcgggtcac cttcaacgcg ctggcgccgt gtctcgtgtt cacgcagctt gtcacgagcc
     3001 gcgtgggggc gggggagttc gggcgcatcg tcgcgttcac gatcctgctg gtggggtcgg
     3061 caggggcggt cgcgcgcctc gcggccattc cgttccggct ggagcgtccc gcgctcgccg
     3121 cgttcctaat cgtcgtcatg ttctccaact ccgcgaacta cggcctctcg gtcgtgctct
     3181 tcgccttcgg cgaggaagcg ctgtcgcggg cggtcgtgta cttcgccacc ggcgcgatgc
     3241 tcgtctacag cgtcgggtcg ttcctcgcgt cgagcggccg gcgcagcgcg cgcgaggccg
     3301 ctgcggggct cctgcgggtg ccggcgctgt ggggcctgtt ggcggctgcc gtcgtgatcg
     3361 ggaccggcgc gacgctcccg gcgtttgtgc tccggcccgc cacgctgttg agctcggccg
     3421 ccatcccgac catgctgctc gtgctgggca tgcagttcga acggggcgcg tggccggagc
     3481 gcccggccct cgtggccact gcggccctgc tcggcctcgt ggggagcccg ctcatcgggt
     3541 ttgggctggc ctggctcctc ggccttgagg gcgtcagccg ccaggccgtg ctcgtcgagg
     3601 ccgccatgcc gagcgccgtc atcaccacca tcctggcggt cgagttcgac gtcgagccgc
     3661 gcttcgtgac gtcggtcgtg gtcgtcacga cgctggccag cgcggtgacg gtgtcgatcc
     3721 tgattgcgct cctgaagtcg gggtactagc agccggggcc tcgcggagca tctccacggc
     3781 tctcgccgcc cacgccgggt ccgcgagcat cgcccgcccg acgcccacga tgtccgcacg
     3841 gcgcgaccgg acgatcacgt cggccgtccg cgggtccgtg atgcctccgg ccacgaggac
     3901 agggacgttc accacgtcct tgagcgcctc ggcgtagggc acgaagtagc cggggcccct
     3961 ccccgggtcg tcagacccgg ccagccctcc agacacgtcg atgagcgacg cgccagcctc
     4021 cacgaggcgc gcggcggtcc agcaggcggg agccagcgtg agcccgcccg gccgctcgtc
     4081 gtgcatgccc aggcgtacga agacggggaa ccccgggccg acccgctgcg tgatcgcggt
     4141 gaccacgtcg gccgtcagcc gcgcccggcg cgccgcgtcg ccgccgtagt cgtcttcgcg
     4201 gcggttggtc aggggggaca ggaactgcga caggaggtag ccgtgcgcgg cgtgcacctc
     4261 gacggcgtcg aagccagcgc gccgggcgcg ggcggcggcg gccgcgaacg cctccacgac
     4321 gttcgcgatc ccgccagtgt cgagcgcggc gggcgtctcc tcgtgcgcct cccggccggt
     4381 ggccacgggc gacgccgaca cgacagggag gccggtcacg cgggaggacg cgcgggaccc
     4441 ggcgtgggcg atctgcaggc aggcgacggc gccctcgccg cggatggcgg cggccaggcg
     4501 cgccagcccg tccgcgcagg cgtcagc
//
LOCUS       DAOSIF010000234         4508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17008_length_4508_cov_3.679542, whole genome shotgun sequence.
ACCESSION   DAOSIF010000234 DAOSIF010000000
VERSION     DAOSIF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17008_length_4508_cov_3.679542"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="PLT35_08520"
     CDS             <1..1626
                     /locus_tag="PLT35_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG29310.1"
                     /translation="THYRVRGLVIHNTRTDLPIKKGLSSSAAICVLTARAFNRIYDLK
                     LTIRGEMELAYAGEITTPSRCGRMDQGCAFGDRAVLMEFDGERLSTEEVRPAKELHFV
                     IVNLNASKDTMEILKRLNRSYPFAEGEVERGVQQLLGPINKRIVGEARAALEAGDAER
                     LGALMTEAQALFDRYAGPACPEELTAPVLHRVLAHEPLQPHVWGGKGVGSQGDGSAQF
                     VARSAADQQAVAEILQRDLGMPALPLTLGTGPKVRRALIPAAGFGTRLFPASKATKKE
                     LFPVVDRDGIAKPAILLIVEEALDAGIEEVVIVVQEEDLAEFSSFFNDQVSVENYNKL
                     PPSSQQYARRILDMGRRVRFVTQPTQQGFGDAVYCARAAIGDEPFLLMLGDHLYRSAS
                     GLSCARQVLDAFQQSGTSVVGLRPTPESEVGSFGVAAGVWIEPGRRLTITEFAEKPTV
                     DYARSRLQVPGLPDGQYLTVFGQYVITPLVFTYLEEHIRHNVRERGEFQLTSALERLR
                     QEDGFLGLIVDGQRFDIGLPSYYLDTLASFSRD"
     gene            1816..2781
                     /locus_tag="PLT35_08525"
     CDS             1816..2781
                     /locus_tag="PLT35_08525"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOG29311.1"
                     /translation="MPGPTLVLLLVLSAAAFAHGASAGDSPPSGPPLAGCSQAAAPLV
                     RSLVAPGAKLEKLAGGFEFAEGPTSDGAGNVFFTDQPNDRILKWSVDGTLTTFLQLAG
                     RANGMFFDADGNLLACADERMELRSIGPDGSHTVLVNRYEGRLLNGPNDVWVRPDGGI
                     YFTDPFYKRPWWSHAAPPQDGQHVYFLAPDRRTVRRVTTDLTKPNGIIGTPDGKTLFV
                     ADIGARRTYAFAIQPDGTLTDKRLHCEMGSDGMTLDEEGRLYLTGRGVSVIDRDGRMV
                     EQFDVPEPWTANVSFGGPDHRTLFITAGTGLYAVRLRVRGANAAK"
     gene            2801..3169
                     /locus_tag="PLT35_08530"
     CDS             2801..3169
                     /locus_tag="PLT35_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29312.1"
                     /translation="MRSLSTLGAALFDLLAPGEACQIAEALSGRIGLLERHVGIGARQ
                     RPGSATRDDEAQDLAIAAAGSSRRAGRGKFPFVWKKPRGGFIRQPKAEFAAAGAEPRG
                     RPDCRRAPGWSHRIPTAGPR"
     gene            3221..>4508
                     /locus_tag="PLT35_08535"
     CDS             3221..>4508
                     /locus_tag="PLT35_08535"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOG29313.1"
                     /translation="MPTAVSSVLMSFVLPVLFALQTGGAAAQAPAPNPELLSRRWQAR
                     WITAAGAEPFAYGVYHFRKSFDLAAPPARFVVHVTADNRYQLFVNGTRVVWGPARGDL
                     NHWRYETVDIAPHLRTGRNVLAAVVWNFSDLAPEAQVTWRTGFLLQGDTAAEQAVNSN
                     ASWKAARNRAYSPIPFTHAQMRGYTVVGPGERVEGAVYPWGWETAGFDDAGWQAAEAA
                     VPDNRDGGSPRDSQDAPNRWLLVPRSIPLMEERPERFASVRQSLGVEVPSSFPAAAAA
                     FTVPPRTRARLLLDMSHLTTAYPVLEYGGGAGATIRLGYAESLYYPRDGERRGEKGNR
                     NEVEGKEFVGNHDVVLADGGRHRAYSPLWWRTYRYVELTIETGMDPLVVEDLRGIYTG
                     YPFERRQRIDTGSDLVSRILDVGWRTARLCAHESYMD"
BASE COUNT          680 a         1574 c         1591 g          663 t
ORIGIN      
        1 acccactacc gcgttcgcgg cctggtcatc cacaacaccc gcaccgacct gccgatcaag
       61 aagggcctgt cctcgagcgc ggccatctgc gtgctcacgg cgcgggcgtt caaccgtatc
      121 tacgacctga agctgacaat ccgtggcgag atggagctgg cctacgcggg cgagatcacg
      181 acgccgtccc gctgcggccg catggatcaa ggctgcgcct tcggcgaccg tgcggtgctg
      241 atggagttcg acggcgagcg gctcagcacc gaggaagtgc ggccggcgaa ggagctgcac
      301 ttcgtcatcg tgaacctgaa cgcgagcaag gacacgatgg agatcctcaa gcggctgaac
      361 cgctcgtacc ccttcgcgga gggtgaagtt gaacgcggcg ttcagcagct gctcggcccc
      421 atcaacaagc ggatcgtcgg cgaggcgcgg gcggcgctgg aagccggcga cgccgagcgc
      481 ctcggcgcgc tcatgacgga ggcgcaggcg ctcttcgacc gctacgccgg gccagcctgc
      541 ccggaggaac tgacggcgcc ggttctgcac cgcgtgctgg cccacgagcc gctgcagccg
      601 cacgtctggg gcggcaaagg cgtcgggtcc caaggggacg ggagcgcgca gttcgtggcc
      661 cgcagcgcgg ccgaccagca ggcggtcgcc gagatcctgc agcgcgatct cggcatgccg
      721 gccctgcccc tgacgctggg cacgggtccg aaggttcgcc gggcgctcat cccggcagcg
      781 ggattcggca cgcggctctt cccggccagc aaggccacga agaaggagct cttccctgtc
      841 gtggaccggg acgggatcgc caagccggcc atccttctga tcgtcgaaga ggcgctcgac
      901 gccggcatcg aagaggtcgt tatcgtcgtc caggaagagg acctggcgga gttttcctcc
      961 ttcttcaacg atcaggtgtc ggtcgagaac tacaacaagc tcccgccttc gtcccagcag
     1021 tacgcccgcc gcattctcga catgggccgc cgcgtccggt tcgtcaccca gccgacgcag
     1081 caggggttcg gcgacgcggt ctattgcgcg cgcgcggcga ttggcgacga gccgttcctc
     1141 ctcatgctcg gcgatcacct ctacaggtcg gcgagcggcc tgtcctgcgc gcggcaggtg
     1201 ctggacgcgt tccagcagag cggcacgagc gtcgtgggcc tgcgccccac gcccgaatcc
     1261 gaggtcggga gcttcggcgt ggcagccggc gtctggatcg agccgggccg gcggctcacc
     1321 atcaccgagt tcgccgagaa gccgacggtg gactacgccc gaagccgcct gcaggtgccc
     1381 ggcctccccg acggccagta cctgacggtg ttcggccagt acgtgatcac gccgctggtc
     1441 ttcacgtacc tggaagagca catccgtcac aacgtccgcg agcgggggga gttccaactc
     1501 acgtcggccc tggagcggct gaggcaggag gacggattcc tgggcctgat cgtagacggg
     1561 cagcgcttcg acatcggtct gccgagctac tacctggata cgctggcgag tttcagccgg
     1621 gactgacgcg caggccgcac gcgtgcgcgc cacggcaccc agcggaccgc cgatgtcgag
     1681 cgcggcgcat ggactcgcgg cggcgcgggt tcccggcgcg gtatcatacc cggcgtcgag
     1741 cgcagatcgg caccatgagg ccggcgcgca cgccgccgcc cgcgatggcc ggggccgcag
     1801 cctcgaagga gcgtcatgcc gggtcccacg ctcgtcctgc tcctagtcct gtcggcggca
     1861 gccttcgcgc acggggcttc cgccggcgac tcgccgccaa gcggtccgcc gctcgcgggg
     1921 tgcagtcagg ccgcggcccc gcttgtccgg agcctcgttg ccccgggcgc gaagctcgag
     1981 aagctcgccg gcggattcga gtttgctgag ggccccacga gcgatggcgc gggcaacgtc
     2041 ttcttcaccg accagccgaa cgaccgcatc ctgaagtgga gtgtggacgg aacgctgacc
     2101 accttcctcc agctggcggg ccgagccaac ggcatgttct tcgacgccga tggcaacctg
     2161 ctcgcctgcg ccgacgagcg catggaattg cggtcgatcg ggcccgacgg ctcgcacacc
     2221 gtcctggtga accggtacga gggcaggctt ctcaacggcc cgaacgatgt ctgggtccgg
     2281 ccggacggcg gcatctactt cacggaccct ttttacaagc ggccctggtg gagccacgcg
     2341 gctccgccgc aggacggcca gcacgtctac ttcctcgccc cggaccgccg gactgtcagg
     2401 cgcgtgacga ccgacctgac gaagccgaac ggcatcatcg ggacgccgga cggcaaaacg
     2461 ctcttcgtgg ccgacatcgg cgcgcgcaga acctacgcgt tcgccatcca gccggacggc
     2521 accctgaccg acaagcgcct gcactgcgag atgggctcgg acggcatgac gctggacgag
     2581 gagggccggc tctacctgac cggccgcggc gtctccgtga tcgatcggga cgggcgcatg
     2641 gtcgagcagt tcgacgtgcc cgagccgtgg accgcgaacg tcagcttcgg cgggccggac
     2701 catcggacgc tgttcatcac ggccggcacg ggcctgtatg cggtgcggct ccgcgttcgt
     2761 ggcgcaaacg ccgcgaagtg atcgatcgcg gagctgcgtc atgcgaagcc tctccacgct
     2821 cggcgccgcc ctgttcgacc tcctggcccc gggcgaggcg tgtcagatcg ccgaggcgct
     2881 gtccggccgt attggccttc ttgagcggca cgtcggcatc ggcgctcggc agcgtccggg
     2941 ctcggcgacc cgcgacgatg aggcgcagga tctcgccatc gccgccgcgg gatccagcag
     3001 gcgggcgggt cgaggcaagt tccccttcgt ctggaagaag ccgcgcggcg gcttcattcg
     3061 gcagccgaag gccgagttcg ccgccgcggg cgccgagccg cgcgggaggc cggattgccg
     3121 gcgcgcgcca gggtggagcc atcggattcc cacggccggt ccccgatagc ggggcgattg
     3181 ccttcgtcgc cacgaccggc cggtttcagc gagggtcgac atgccgactg ccgtctcgtc
     3241 cgttctgatg tcgttcgtcc tgccggtcct gttcgccctg cagactggcg gcgcggccgc
     3301 ccaggctccg gcgccgaatc ccgaactcct ctcgcggcgg tggcaggcgc gctggatcac
     3361 ggcggccggc gccgagccgt tcgcctacgg cgtgtaccac ttccgcaagt cgttcgacct
     3421 ggcggcgccg ccggcgcgct tcgtcgttca cgtcacggcc gacaaccgct accagctctt
     3481 cgtcaacggc acgcgcgtcg tgtgggggcc ggcccgaggc gacctcaacc actggcggta
     3541 cgagacggtg gacatcgccc cgcacctccg gaccgggcgg aacgtgctgg cggccgtggt
     3601 ctggaacttc tccgatctcg cccccgaagc gcaggtgacg tggagaacgg ggttcctgct
     3661 ccagggcgac acggcggccg agcaggcggt gaacagcaac gcctcgtgga aggccgccag
     3721 gaaccgcgcc tactccccca ttccgttcac ccacgcccag atgcgcggct acaccgttgt
     3781 cgggcccggc gagcgcgtcg agggcgcggt gtacccctgg ggctgggaaa cggcaggctt
     3841 cgacgacgcg ggatggcagg cggccgaggc ggccgtgccg gacaaccggg acggcggatc
     3901 tccgcgggac tcgcaggacg cgccgaaccg ctggctgctg gttccgagga gcattccgct
     3961 catggaggag cggccggagc ggttcgccag cgtgcgccag tcgctgggtg tggaggtgcc
     4021 gtcctccttt ccggcggcgg cggccgcgtt cacggtcccg ccccgcaccc gggcgcggct
     4081 gctgctcgac atgtcgcacc tgacgacggc ctacccggtg ctcgagtacg gcggcggcgc
     4141 cggtgcgacg atcaggctgg ggtatgccga gtcgctctac tatcccaggg acggcgagcg
     4201 ccgcggtgag aagggcaatc gcaacgaggt cgaaggcaag gagttcgtcg gcaaccacga
     4261 cgtggtgctg gccgacggcg gccggcaccg cgcctacagc ccgctctggt ggcgcacgta
     4321 ccgctacgtc gagctgacga tcgagacggg gatggatccg ctcgtcgtgg aggacctccg
     4381 gggcatctac acgggctacc cgttcgaacg gcgccagcgc atcgacaccg ggtccgatct
     4441 cgtgtcgcgc atcctcgacg tggggtggcg gacggcgcgg ctctgcgcgc acgagagcta
     4501 catggact
//
LOCUS       DAOSIF010000235         4482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17158_length_4482_cov_3.397335, whole genome shotgun sequence.
ACCESSION   DAOSIF010000235 DAOSIF010000000
VERSION     DAOSIF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17158_length_4482_cov_3.397335"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLT35_08540"
     CDS             complement(<1..1184)
                     /locus_tag="PLT35_08540"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOG29314.1"
                     /translation="MRVGLWGCVLVFAGIAASCARQEPPAPPAQAGAPSAGAAAARAG
                     RSYRFDRNGWTFVHLEGAPAEIGFQHGYRLAPEIDDLLRVMKPFLLETSKRDWAFYRN
                     VAETVLWPGIDAEYRAEIDGIVDGLAARGVAADRWDLAALNAFEEVPDYYVPWLDAQQ
                     GRPPVGRAPGNCSAFVATGDWTRDGRIVMGHNAWTTYVTGARWTIVFDIVPERGHRIL
                     MDALPGLIASDDDFGVNGAGIMITETTITGFKGFDPAGTPEFFRARKAMQYAESIDDV
                     VRIMRERNNGGYANDWLIGDAKTGEIALFELGLKNSTVDRTNNGYFVGANFPVKEKLT
                     REETDFDPANAASSPNARRARWEQLMAEHKGRIDIEAAKAFEADRYDVIERRDGPSER
                     SL"
     gene            1441..2466
                     /gene="pheS"
                     /locus_tag="PLT35_08545"
     CDS             1441..2466
                     /gene="pheS"
                     /locus_tag="PLT35_08545"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG29315.1"
                     /translation="MAVDFDLIVAEGLAALAAADGSGALERWRVAHLGRSAPVMVALG
                     TLGALPKEDRRAFGQRGNQARQALERAYEEAAARVRQAELSRDLEAGAVDVTAPGRRR
                     GRGRLHPTTRTLRRIYAIFGEMGFQIYRSRDVETDEYNFSLLNIPPHHPARDMWDTFY
                     TTAPNVILRTHTSPGQIHVMRERCPEPIRVILPGMCYRYEQITARSDIQFYQVEGLAI
                     GRRITMADLKGTLTAFAHALFGSEVRTRFRADHFPFTEPSAEMDVECFVCEGQGCPVC
                     KQSGWLEILGCGMVHPVVLQNGGYDPREFSGFAFGMGPQRITMLMHKIEDIRYFFSND
                     VRFLEQF"
     gene            2549..>4482
                     /gene="pheT"
                     /locus_tag="PLT35_08550"
     CDS             2549..>4482
                     /gene="pheT"
                     /locus_tag="PLT35_08550"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG29316.1"
                     /translation="MRVPLTWLRDYVAITDTPDELASRLTYAGLEVEGIEYVGLAPVT
                     RPVAGLPAAGRSGPAARGLAWDPATIVIARILEVLPHPNADRLTLLRVDDGSGAEPVV
                     LTGAPNVFHLKGAGPLPKPLKIVYAREGAVLIDGHKSGREIITLKRAKIRGVESSSMA
                     CSEKELGISDEHDGIIILDDDAPVGAAAAEYMGDVVFEIKINPNMARNACVLGIAREI
                     AALTGAPLKPPALGVTQAGPPIAGRVGIEIREPDLNPRFVLGLIEGVTIAPSPYWVRR
                     RLRLAGTRPINNIVDVTNYVMFELGEPLHAFDFDALVRRADGRRPTIITRRAGPGERI
                     TTLDGVERALDPFTILVCDEAGPLSIAGVMGGAETEVADTTANVLLEGAAWNLVNVRR
                     TMKAQDLPSEASSRFARGVHPGLAEKGVVRALELMRQWAGGTVARGLVDSYPGRPGPV
                     VVDITPAEVERQLGLRVPAGEIVRILRALEFSCEVVGDASDPAAVVRATAPDHRLDIG
                     SGHTGKADLVEEIARIHGYERIPETMLADVVPPPHPNRDLEREEQARDVLVSLGLQEI
                     VTYSLTSPEREARLALAARPEGGAEIPYVRLANPIVVDRVVMRRAILPGLIEAAAANS
                     RNTDRMAFFEIGNVFLPRPE"
BASE COUNT          627 a         1605 c         1600 g          650 t
ORIGIN      
        1 cacagggacc gctcggacgg gccgtcgcgc cgctcgatca cgtcgtagcg gtcggcctcg
       61 aaggccttgg ccgcttcgat gtcaatgcgg cccttgtgct cggccatcag ctgctcccag
      121 cgcgcgcggc gcgcgttggg cgaactggcc gcgttcgccg gatcgaagtc cgtctcctcc
      181 ctggtcagct tctccttcac ggggaagttg gccccgacga agtagccgtt gttcgtgcga
      241 tcgaccgtcg agttcttcag gcccagctcg aacagcgcga tctccccggt cttggcgtcg
      301 ccgatcagcc agtcgttcgc gtagcccccg ttgttccgct cccgcatgat gcgcacgacg
      361 tcgtcgatgg actcggcgta ctgcatggcc ttgcgggcgc ggaagaactc gggcgtgccg
      421 gcgggatcga aacccttgaa cccggtgatg gtggtctccg tgatcatgat tcccgcgccg
      481 ttcacgccga agtcgtcgtc gctcgcgatg aggccgggca gagcgtccat caggatgcgg
      541 tgcccccgct cgggcacgat gtcgaagacg atcgtccacc gcgcccccgt cacgtacgtc
      601 gtccacgcgt tgtggcccat gacgatgcgg ccgtcccggg tccagtcgcc ggtcgcgacg
      661 aacgcgctgc agttgccggg cgcccggccc acgggcggcc gcccctgctg cgcgtcgagc
      721 caggggacgt agtaatcagg cacctcctcg aaggcgttca gcgcggccag gtcccagcga
      781 tcggcggcga cgcccctggc ggcgaggccg tcgacgatcc cgtcgatctc ggcccggtac
      841 tcggcgtcga tgcccggcca cagcaccgtc tccgccacgt tgcggtagaa ggcccagtcg
      901 cgcttggagg tctcgaggag gaagggcttc atcacgcgca gcaggtcgtc gatttcgggt
      961 gcgagccggt acccgtgctg gaagccgatc tccgctgggg cgccttccag gtgcacgaag
     1021 gtccatccgt tccgatcgaa ccggtagctc cgcccggcgc gcgcggcggc tgcgccggcg
     1081 ctcggggcgc cggcctgcgc aggcggcgcc gggggctcct gcctggcgca cgaggccgcg
     1141 atcccggcga acacgagcac acacccccag agtcccacgc gcatgtcagt cctcccgcgt
     1201 gtccggccgc gacccggcag cggccccgcg gtacggtagt gcaggtacgg ccgaggatca
     1261 atccgtacgc gcccccaggg cagcgcgcac aggccgtgcc ggcggcctgc cggggccatg
     1321 cgcccgccgt ttccgagccg cggccgctcc gatccgggct cgctcggccg cgggcgggtc
     1381 cggcgccggg tggcgctcga cccgccgccg atgtatcgta tgcgtctggc gagtgtgcgc
     1441 atggcggttg acttcgacct catcgtggct gaggggctgg cggcgctggc ggcggcggac
     1501 gggtccggcg cgctggagcg gtggcgcgtc gcgcacctcg gcaggagcgc gccggtgatg
     1561 gtggcgctcg ggaccctcgg cgcgctcccg aaggaggatc gccgggcgtt cgggcagcgc
     1621 ggcaaccagg cgagacaggc gctcgagcgc gcctacgagg aggcggcggc gcgggtccgg
     1681 caagccgagt tgtcgcgcga cctcgaggcc ggggccgtgg acgtgacggc gccggggcgg
     1741 cgccgggggc gcggccggct ccacccgacc acgcggacgc tgcggagaat ctacgcgatc
     1801 ttcggggaga tgggcttcca gatctaccgc tcgcgcgacg tcgagacgga cgagtacaac
     1861 ttcagcctgc tcaacatccc cccgcaccac ccggcgcggg acatgtggga caccttctac
     1921 acgacggcgc ccaacgtcat cctgcggacg cacacgtcgc ccgggcagat ccacgtgatg
     1981 cgggaacgct gccccgagcc gatccgcgtc atcctgccgg gcatgtgcta ccgctacgag
     2041 cagattaccg cgcgcagcga catccagttc taccaggtcg agggcctcgc catcggccgg
     2101 cgcatcacga tggccgatct gaaggggacg ctgacggcgt tcgcgcacgc gctgttcggg
     2161 tccgaggtcc gcacgcggtt ccgggcggac cacttcccgt tcaccgagcc ctcggcggag
     2221 atggacgtcg agtgcttcgt gtgcgagggg cagggctgtc cggtctgcaa gcagagcggg
     2281 tggctggaga tcctgggctg cggcatggtg cacccggtgg tgctgcagaa cggcggctac
     2341 gatccccgcg agttctccgg cttcgcgttc gggatgggcc cgcagcggat caccatgctg
     2401 atgcacaaga tcgaggacat ccggtacttc ttctcgaacg acgtgcggtt cctggagcag
     2461 ttctgacgat ccgggcgcgc tgcgggcgcg ccgcggctgg cgggccgcgg gcgcacaggg
     2521 cgggcccagt gggcaagcga gactgacgat gcgcgttccg ctgacctggc tccgcgacta
     2581 cgtcgcgatc accgacacgc cggacgagct ggcctcccgg ctgacctacg ccggactcga
     2641 ggtcgagggc atcgagtacg tcggcctggc gcccgtcacc cgccccgtcg ccggcctgcc
     2701 ggccgcgggc cgctcgggcc ccgcggcccg aggcctcgcc tgggaccccg ccacgatcgt
     2761 catcgcccgg atcctcgagg tgctgccgca cccgaacgcg gaccgcctca cgctgctccg
     2821 cgtggacgac ggctcgggcg ccgagcctgt cgtgctgacg ggcgcgccga acgtctttca
     2881 cctgaaaggc gcgggccctc tcccgaagcc gctgaagatc gtgtacgccc gcgagggcgc
     2941 cgtgctcatc gacggccaca agagcggccg cgagatcatc acgctgaagc gcgccaagat
     3001 ccgcggcgtc gagtcgtcgt cgatggcgtg ctcggagaag gagctcggca tctcggacga
     3061 gcacgacggc atcatcatcc tggacgacga cgcgccggtc ggggccgcgg cggccgagta
     3121 catgggcgat gtcgtgttcg agatcaaaat caatccgaac atggcgcgca acgcgtgcgt
     3181 cctgggcatc gcccgcgaaa tcgccgccct gaccggggcc cccctgaagc ccccggccct
     3241 gggcgtgacc caggccgggc cgcccatcgc gggacgggtc ggcatcgaga tccgcgagcc
     3301 cgatttgaac ccccgcttcg tcctcgggtt gatcgagggc gtcaccatcg cgccgagccc
     3361 ctactgggtc cgccgccgcc tccgcctggc cggcacccgg ccgatcaaca acatcgtgga
     3421 tgtgaccaac tacgtgatgt tcgagttggg cgagccgctc cacgcattcg acttcgacgc
     3481 cctcgtgcgc cgcgcggacg gccggcggcc gacgatcatc accaggcggg cggggcccgg
     3541 cgagaggatc acgacgctgg acggcgtcga gcgggcgctc gaccccttca cgatcctggt
     3601 ctgcgacgag gccgggccgc tgtcgattgc cggcgtcatg ggcggcgcgg agaccgaggt
     3661 cgccgacacg acggccaacg tgctccttga aggcgcggcc tggaacctcg tgaacgtgcg
     3721 gcggaccatg aaggcgcagg acctgccgtc ggaagcctcc tctcgcttcg cgcgcggcgt
     3781 ccatccgggc ctcgccgaga agggcgtcgt ccgggcgctg gagctgatgc gacagtgggc
     3841 cggcggcacc gtggcccgcg gcctcgtgga cagctatccc gggcggccgg gtccggtcgt
     3901 cgtcgacatc accccggccg aagtcgaacg ccagctcggc ctgcgcgtgc cggccggcga
     3961 gatcgtgcgg atcctgcgcg ccttggagtt ctcctgcgag gtcgtcggcg acgcctcgga
     4021 ccccgccgcg gtggtccggg cgacggcgcc ggaccaccgg ctggacatcg gatcgggcca
     4081 caccggcaag gccgatctgg tcgaggagat cgcgcgcatc cacggctacg agcgcatccc
     4141 cgagaccatg ctcgccgacg tggtgccgcc gccccatccg aaccgcgatc tggagcgcga
     4201 ggagcaggcg cgggacgtcc ttgtcagcct cggcctccag gagatcgtca cctactcgct
     4261 gacctcgccg gagcgcgagg cgcgcctcgc gctggcggcg cggccggagg gcggcgccga
     4321 gatcccgtac gtccggctcg cgaaccccat cgtcgtcgat cgagtggtga tgcggcgagc
     4381 catcctgccc ggcctgatcg aggcggcggc ggccaacagc cgcaacaccg atcggatggc
     4441 cttcttcgaa atcggcaacg tcttcctgcc gaggccggag ga
//
LOCUS       DAOSIF010000236         4470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17206_length_4470_cov_3.329332, whole genome shotgun sequence.
ACCESSION   DAOSIF010000236 DAOSIF010000000
VERSION     DAOSIF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17206_length_4470_cov_3.329332"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..622
                     /gene="dtd"
                     /locus_tag="PLT35_08555"
     CDS             173..622
                     /gene="dtd"
                     /locus_tag="PLT35_08555"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG29317.1"
                     /translation="MRAVVQRVSRARVLVGGETAGEIGPGLLALVGVGADDGPADAAY
                     IAGKIRGLRVFEDAGGKMNRSLGEAGGAVLAVSQFTLYGDCRKGRRPSFDHAAPPDAA
                     RRLFEAVVSDLRAAGVPVETGRFQARMAVELVNDGPVTILLDSRRTF"
     gene            complement(665..2284)
                     /locus_tag="PLT35_08560"
     CDS             complement(665..2284)
                     /locus_tag="PLT35_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG29318.1"
                     /translation="MRRDFSRLAGRPFDVLIVGGGIHGLAAAYDAAQRGFSTALVERA
                     DFGSGSSFNHAKTVHGGLRSLQTGDVRKARFSICERRAMARIAPHLVTPLAFAMATTR
                     KLTRSTWALRAGFLADAAIGYDRNAAVPPGLRLPAGRVIGLQEYRRAFGADAQPEATG
                     AATWWDYQMPFSDRLTFAFAQAAAASGAVLVNYAEALEPLMTGRRVRGARVCDGLDGG
                     VVEVEARVTVNAAGAACRPWMERLGGSPSFPLLKAMNLVTSRPAGPLALSAPTSGGRL
                     LLIMPWQGRMLIGTSHSDRPAEPGDLAVGCAEVSAFVAEVNSAFPALRLRQDEVTLVH
                     RGVVPAVRDRRGALGLMGHHRVHDHEADGVSGAVSMIGVKYTTGRGVAEQAVGLLAGK
                     LGRSGPPCRTGITRLPGADLDSLEAETARAASETRGLFGSDSLAAVVATHGTRWRQVA
                     ALCRADPSLAEPIGGGVPFPQAAVVHAVRDEMACTLADVVLRRLPIGAAARPDPAAVS
                     ACAALMAAECGWGPDRVASEIQALDSFYAIE"
     gene            2347..3129
                     /locus_tag="PLT35_08565"
     CDS             2347..3129
                     /locus_tag="PLT35_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG29319.1"
                     /translation="MTGRPARVLPTTADVRAYWDRRIHDLEVSRHPPGTPGFFADLDE
                     YHFDKLHHLLRLVDYGGYAGRRVLDVGCGAGVDLARFAGAGARAVGVDVSESAVALAR
                     RNLAQRRLDGLLCPADAEALPFPDETFDFVFAHGVVQYACGDQAVVDECRRVVKRGGT
                     VFIQVYNRVSWLNALSRLMKVGLEHEDAPVLRKYSPGEFRRLLRGFAHVRVLPERFPV
                     RSRLHRGWKGAVYNGLFVGVFNALPRRLVRRFGWHLLAFCRK"
     gene            3158..3961
                     /gene="dapF"
                     /locus_tag="PLT35_08570"
     CDS             3158..3961
                     /gene="dapF"
                     /locus_tag="PLT35_08570"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG29320.1"
                     /translation="MELLLAKAHAYGNDFIYLESADLARADGPELARTLCERHTGVGA
                     DGLILFRQTSSGAVMRLYNADGSPSEVSGNGVRGLAALLCWRTRQHGTAAPGTLAIGT
                     DAGVKVVSVLEAVPPRFLCRSAMGQPAGLRRETLEVAGDSVAVVTLRVGNPQCVVLES
                     RLDEARYRRLGPALERHAAFPEGTNVEFAAVEGPRRVRVLIWERGVGPTRSSGTGACA
                     AAVAAMAYAGVERDAEIVSPGGAQRVEWNESGLFLTGWADMVWEGRWIR"
     gene            3958..4095
                     /locus_tag="PLT35_08575"
     CDS             3958..4095
                     /locus_tag="PLT35_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29321.1"
                     /translation="MIRNRRQALELAEPRLPAERLSSHWRKTAGLVLGALLFVRAWPL
                     R"
     gene            4261..>4470
                     /locus_tag="PLT35_08580"
     CDS             4261..>4470
                     /locus_tag="PLT35_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29322.1"
                     /translation="MTLTVLMKLLWPRRPERQRLQILPARPQRRGQSVASPTRRDSRR
                     AAPSDATIAACTRRIHRLRPLRPVHA"
BASE COUNT          611 a         1624 c         1598 g          637 t
ORIGIN      
        1 ccagccggcc ctccgcgttg accgccgcca gcccgcaccc gggcgtcagg gcgtcgatgc
       61 tgcgggccct cgcgaatgtc gcgcccagcc acctgctgga caggcgcttg atagcgggcg
      121 agcgcccgac aggcggccgg gcgagcagcg gcgatgccga gcaggagcgc gcatgagagc
      181 cgtcgtgcag cgggtgagcc gggcgcgcgt gctcgtcggc ggcgagacgg cgggggagat
      241 cggaccgggg ctcctggccc tggtcggcgt cggcgccgac gacgggccgg ctgacgccgc
      301 ctacatcgcg ggcaagatcc gcggtctccg cgtgttcgag gacgctggcg gcaagatgaa
      361 tcgatcgctc ggcgaggccg gaggcgccgt cctggccgta tcgcagttca cgctgtacgg
      421 cgactgccgg aagggcaggc gcccgtcgtt cgaccatgcg gccccgcccg acgccgcccg
      481 gcgcctgttc gaggcagtcg tgagcgacct gcgcgcagcc ggagtcccgg tcgaaacggg
      541 acggttccag gcccgcatgg ccgtggagct ggtcaacgac ggcccggtga cgatcctgct
      601 cgacagccgc aggacgtttt gagacgccag ccggcccgct cgggcgagcc gcagggccgt
      661 cgcctcactc gatcgcgtag aagctgtcga gcgcctggat ctccgacgcc actcggtccg
      721 gaccccatcc gcactccgcc gccatcagcg ccgcgcacgc ggatacggcg gccgggtccg
      781 gccgagccgc cgccccgatt ggcagccgcc tcaagacgac gtcggcaagc gtacacgcca
      841 tctcgtcgcg cacagcgtgc acgaccgcgg cttgggggaa cggcacgccg ccgccgatcg
      901 gctcagccag cgacgggtcc gcgcggcaca gggccgcgac ctggcgccag cgcgtcccgt
      961 gcgtcgccac gacggcggcc agcgagtcag agccgaacag gccgcgcgtc tccgacgccg
     1021 cccgcgcggt ctccgcctcg agactgtcca ggtcggcgcc gggcagccgg gtgatccctg
     1081 tgcggcacgg cgggccggag cgaccgagct tgccggcgag cagccccacc gcctgctcgg
     1141 cgacgcctcg gccggtcgtg tacttcacgc cgatcatcga gacggcgccc gagacgccgt
     1201 cagcctcgtg atcgtgcacg cggtggtgcc ccatcaggcc gagcgcgcca cgccgatcgc
     1261 gcaccgccgg aacgacgccg cgatggacga gcgtcacctc gtcctgccgg agcctgagag
     1321 ccgggaacgc cgagttcact tcggccacga acgccgacac ctcggcgcag ccgaccgcca
     1381 gatctcccgg ctccgcgggc cgatccgagt gagacgttcc aatcagcatg cggccctgcc
     1441 acggcatgat gagcaggagg cgaccgccgg aggtcggggc gctcagggcg agagggccgg
     1501 ccgggcgcga tgtcacgagg ttcatcgctt tcagcagggg gaaactcgga ctgccgccca
     1561 gtctctccat ccacgggcgg catgcggcac ccgcggcgtt gacggtgacg cgagcctcga
     1621 cctcgacaac gcctccgtcc aggccgtcgc agaccctggc gccccgcacc cgccggcccg
     1681 tcatcagcgg ctcgagggcc tcggcatagt tgacgagcac cgcgccgctg gcggcggccg
     1741 cctgcgcgaa cgcaaacgtc aggcggtcgg aaaagggcat ctggtagtcc caccacgtgg
     1801 cggcccccgt ggcctccggc tgcgcatcgg ctccaaaggc gcgccggtac tcctggaggc
     1861 caatcacccg acccgccggg aggcgcaggc ccggcggcac ggcggcgttg cggtcgtagc
     1921 cgatggctgc gtcagccagg aagccggccc gcagggccca tgtggaccgc gtgagcttcc
     1981 tggtggtcgc catcgcgaac gccagaggcg tgaccaggtg cggcgcgatg cgcgccatcg
     2041 cccggcgttc gcagatcgag aacctggcct tgcgcacgtc gccggtctgg agcgagcgca
     2101 gcccgccatg cacggtcttc gcgtggttga acgaactgcc gctgccgaaa tcggcccgct
     2161 cgaccagcgc ggtcgagaag ccacgctgcg ccgcgtcgta ggccgccgcc agtccgtgga
     2221 tgccgccgcc gacgatgagc acgtcgaacg gccggcctgc caagcgcgag aagtcacgtc
     2281 tcatgaggct gtcggctgct gccggctcgt tgccggaagc gcccgggtat tctatagtac
     2341 gccctcgtga ccggccgtcc cgcgcgcgtc ctgccgacca cggccgacgt ccgcgcgtac
     2401 tgggatcgcc gcatccacga ccttgaggtc agccgtcacc ccccgggcac gccgggtttc
     2461 ttcgcggacc tcgacgagta tcatttcgac aagctccatc atcttctgcg cctcgtcgac
     2521 tacggcggat acgccggccg gcgcgtcctc gatgtcggct gcggcgcggg cgtggatctg
     2581 gcgcgattcg ccggggccgg ggcgcgggcc gtcggtgtgg acgtgtcgga atcggccgtt
     2641 gcgcttgccc gacggaacct cgcgcagcgg cggctcgacg gccttctgtg cccggcggac
     2701 gccgaggccc tgccgtttcc cgacgagacg ttcgacttcg tgttcgccca cggcgtcgtg
     2761 cagtacgcct gcggggacca ggccgtcgtg gatgaatgcc ggcgcgtcgt gaagcggggc
     2821 ggaacggtct tcatccaggt ctataaccgg gtctcgtggc tgaacgccct ctcgcgcctg
     2881 atgaaggtcg gtctcgagca cgaggacgct ccggtgctcc gcaagtacag ccccggcgag
     2941 ttccgccgtc tcctgcgcgg ctttgcccac gtccgcgtcc tgcccgaacg cttcccggtc
     3001 agatccaggc tccaccgagg ctggaagggc gccgtgtaca acggcctctt tgtgggagtc
     3061 ttcaacgcgc tgcccaggcg gctcgtgagg cgattcggct ggcacctgct ggcgttctgc
     3121 cggaagtagg actcactcca acgcccgggg tggcgacgtg gaactgctgc tggccaaggc
     3181 ccacgcgtac ggcaacgact tcatctacct ggagagcgcc gacctcgccc gcgcggacgg
     3241 gccggagctg gccagaaccc tgtgcgagcg ccacacgggc gtgggcgccg acgggttgat
     3301 cctcttccgc cagacctcca gcggcgcggt catgcggctc tacaacgcgg acggcagtcc
     3361 gtcggaggtc tcgggcaacg gcgtccgtgg cctggcggcc ctgttgtgct ggcgtacgcg
     3421 gcagcacggg acggcagccc ccgggaccct ggcgattggc accgacgcgg gcgttaaggt
     3481 cgtcagcgtc ctcgaagcgg ttccgccgcg cttcctgtgc cggtcggcga tgggccagcc
     3541 cgccggactc cgacgcgaga cgctcgaggt cgctggggac agcgttgcgg tggtgaccct
     3601 ccgggtcggc aatccccagt gcgtcgtgct cgaatcccgc ctggacgagg cgcgctatcg
     3661 caggctcggc ccggcgctcg agcgccacgc cgccttccct gaaggcacca atgtcgagtt
     3721 cgccgcggtc gaagggccgc ggagggtccg cgtgctgatc tgggaacggg gcgttggccc
     3781 gacgcgctct tccggaaccg gtgcgtgcgc ggcggctgtg gcggcgatgg cctacgcggg
     3841 cgtcgaacgc gacgcggaga tcgtgtcccc gggaggcgca cagcgcgtcg agtggaacga
     3901 gtccgggctg ttcctgacag gatgggccga catggtctgg gagggccgct ggattcggtg
     3961 attcgaaacc ggcgccaggc gctcgagctg gccgaacccc gattgccggc cgagcgcctg
     4021 tccagccact ggcggaagac ggcgggactc gtcctcggtg cgctgctgtt cgtgcgcgcc
     4081 tggccgctcc gctgacgagt ctgccgccgc cggcccgacg ccgcggcatc ttcggcgtga
     4141 tgcctgtgtt cctcccgatg cgggtcaaga accgcgccac cgcggccacg gtgttcccga
     4201 tcggcctcac gggcgtcggc cgcttgttct tccacggtga ggctctcggt tgcggcgtgc
     4261 gtgacgctca ccgtcctgat gaagctgctg tggccgcgcc ggcctgaacg ccaacggctg
     4321 cagattctgc ctgcccggcc gcagcggagg gggcaatcgg tggcatcacc gactcgccgc
     4381 gattcccggc gggctgcgcc gagcgacgcg acgatcgccg cctgcacccg ccgcatccac
     4441 cgcttgcgcc ccctccggcc tgtccacgcc
//
LOCUS       DAOSIF010000237         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17248_length_4459_cov_3.958901, whole genome shotgun sequence.
ACCESSION   DAOSIF010000237 DAOSIF010000000
VERSION     DAOSIF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17248_length_4459_cov_3.958901"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1423
                     /locus_tag="PLT35_08585"
     CDS             260..1423
                     /locus_tag="PLT35_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29323.1"
                     /translation="MPRALWGAALVAAVSFVSACSDKTTPIKVVESSPSAPSGPSTGT
                     ATLAAPVPDAPVDNVQLTTLRPTLSVRNVPSTQAGGAKLYEFQIADNPSFSADSGASS
                     LYYPVAVTKTGVAEDASGKTGFTVDADLQPATRLYWRARVAQGTAVSDWSATATFRTR
                     IAGYSRAGELYDPLVNGETVGERVGATAFVPGDGLRLDSSSSYVRYRLPQAVSAGEFS
                     MEVKGLRPNNPIAQGFSSNKPKIFGMQDGDGDYTTNLWRIDAQYRGLEGNPPNCIHFR
                     AMFGGNQFKIEPDTAVRYSMVFLLDPAKAYFWKGSWSNGFRLEVFDGLGGAKLYDQSL
                     PVSNAYNPPRHVAYLGAPVGSGGEMSVIAGTTYRNVWLGARAKPASLGSALER"
     gene            complement(1439..2395)
                     /locus_tag="PLT35_08590"
     CDS             complement(1439..2395)
                     /locus_tag="PLT35_08590"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG29324.1"
                     /translation="MVHYEFRVEGEEAGQRLDHFLVSKLNGPSRSQVQRLIKDGLVAV
                     GGRGSKANYTVRAGDAVAADLPDPAPAAPQPEALPLDVLFEDADVIVVNKAAGMVVHP
                     APGHASGTLVNALLHHVDDLSGVGGELRPGIVHRLDRGTSGVMVVAKHDASHRELARQ
                     FHDREVEKEYEALVWGLVQQGRRIDAAIGRDPRDRTKMSHRARYARSAATRVTLARFL
                     NGVSYLRVVIATGRTHQIRVHLSGIGHPIVGDTVYGGVRKQMPARLRPVLRLERPFLH
                     ARRLAFTHPADAARRLEFTAPLPADLQAVLDELEAHARAGGT"
     gene            complement(2466..3293)
                     /locus_tag="PLT35_08595"
     CDS             complement(2466..3293)
                     /locus_tag="PLT35_08595"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG29325.1"
                     /translation="MLPRLLELGPFTVYSYGVLLASAYLLALYVAFRRARRFGLDATR
                     VLDLGVYIIIAALIGAKLLLLIVDFDHFRRHPGEIWTLVRTGGVFYGGLIVAFGVGLW
                     YVRRHHLPLWPVADAVAPGLALGHVVGRFGCLLAGCCYGKPTTVPWAVTFTDPFAASN
                     VGTPLHVPLHPTQLYDAGAEFLILLFLLATERRWRRRAGWTWWTYILLYGISRYVIEM
                     FRGDPRGAAMGLSTSQWIALGLVPLSIAMLAVLWRRGPAAAPAGPAPAAPRRKRRGR"
     gene            complement(3274..3798)
                     /gene="lspA"
                     /locus_tag="PLT35_08600"
     CDS             complement(3274..3798)
                     /gene="lspA"
                     /locus_tag="PLT35_08600"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOG29326.1"
                     /translation="MSAAADAGPACRRAVRLEAALAAAVILLDQLTKALVRREIPLHD
                     SVPVVPGVLNLVHVRNTGAAFGFLNDVDFPGKAAVVAVVATGALIGIALYAARVSSRQ
                     RLARAGLALIIGGAVGNLIDRLAFGSVVDFVDVVFGAWHFWAFNVADSAITVGVAAML
                     ADMMGLNRHAPSTP"
     gene            complement(3795..>4459)
                     /locus_tag="PLT35_08605"
     CDS             complement(3795..>4459)
                     /locus_tag="PLT35_08605"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG29327.1"
                     /translation="AFVAVDLSAFYVDVSKDRLYTLAPGSRGRRSAQTAMFAIADGLA
                     RLIAPILPVLADEYWASLPGEREESVHLAEFPTRVELFRDAELVARWSRLLKLRAAVN
                     AELERLRQAKAIGQSLEAAVHLRGTGALADLIASYRDELPGLFITSQVDGETDAPSAG
                     AEPEAGAIYREDEHSAVHIVAERAAGVKCERCWRYVPAVSSDPEREGVCPRCEAVLAA
                     AR"
BASE COUNT          661 a         1633 c         1582 g          583 t
ORIGIN      
        1 gtgacggacc atgtcgagcg tcgccgtccg gccgttgggc aggcgcaccc ggtcgacggt
       61 gatcgtgaaa atccggccct tgaacgcctc cgaactcgag agcagcgtcg ctgcgcgttc
      121 ctccatgctc gccttcctcc gtttcgccgc ctgccgtccc cgctggttcg cgccgcgggg
      181 cgtacgctaa gataccttgc tcgccgccgc cgcggggcgc tccgtcaccg gggcggcggc
      241 ccgatccgag gaggccgccg tgccgcgtgc attgtgggga gccgccctgg tggcggccgt
      301 ttcgttcgtg tcagcgtgca gcgataagac cactccgatc aaggtcgtcg aatcctcccc
      361 ctcggcgccg agcggcccgt cgaccggcac ggcgacgctg gccgccccgg tcccggacgc
      421 gccggtggac aacgtccagt tgaccacgct tcgccccacg ctgagcgtgc gcaacgtgcc
      481 ctcgacccag gccggcggcg cgaagctcta cgagttccaa atcgccgaca acccgtcctt
      541 ctcggccgac agcggcgcct ccagcctcta ctaccctgtg gccgtcacca agaccggcgt
      601 ggccgaggac gcgagcggca agacagggtt caccgtggac gcggacctcc agccggccac
      661 ccgcctgtac tggcgcgcgc gcgtcgcgca ggggaccgcc gtctcggact ggtcggccac
      721 ggcgacgttc aggacgcgga tcgccggcta cagccgggcg ggggagctgt acgacccgct
      781 ggtcaacggc gagaccgtgg gcgaacgcgt gggggccacg gcgttcgtgc ccggcgacgg
      841 gctccgcctc gactcgtcca gcagttacgt gcggtaccgc ctgccccagg ccgtcagcgc
      901 cggcgagttc tcgatggagg tgaaggggct ccggcccaac aacccgatcg cccagggctt
      961 ttccagcaac aagccgaaga tcttcggcat gcaggacggc gacggcgact acacgacgaa
     1021 cctctggcgc atcgacgccc agtaccgcgg cctcgagggc aatcctccca attgcatcca
     1081 cttccgcgcg atgttcggcg gcaaccagtt caagatcgag ccggacacgg ccgtccgcta
     1141 ctcgatggtg ttcctgctcg atcccgcgaa ggcgtacttc tggaagggca gctggagcaa
     1201 cggcttccgg ctcgaggtgt tcgacgggct cggcggcgcg aagctctacg accagtcgct
     1261 cccggtgtcg aacgcctaca acccgccgcg ccacgtggcc tatctgggcg cgccggttgg
     1321 cagcggcggc gagatgtccg tgatcgccgg cacgacctac cgcaacgtct ggctcggcgc
     1381 ccgggcgaag ccggcctcgc ttggcagcgc gctcgagcgc tgacgggcgg aggcgccgtc
     1441 aggtcccgcc ggccctggcg tgcgcctcca gttcgtccag gacggcctga agatcggcgg
     1501 gcagcggcgc ggtgaactcg aggcgcctcg ccgcgtcggc cgggtgcgtg aacgccaggc
     1561 gccgtgcgtg aaggaagggg cgctccagcc gcagcaccgg ccgcaggcgg gcgggcatct
     1621 gcttccgcac gcccccgtag acggtgtcgc cgacaatggg gtggccgatg ccgctcaggt
     1681 gcacccggat ctggtgggtg cggccggtcg cgatgacgac gcgcaggtag gagacgccgt
     1741 tgaggaaccg ggcgagcgtc acgcgcgtgg cggccgaccg ggcgtaccgc gcgcggtggg
     1801 acatcttcgt gcggtcgcgc gggtcgcggc cgatggccgc gtcgatccgg cgcccctgct
     1861 gcaccagccc ccacacgagg gcctcgtatt ccttctcgac ctcgcggtcg tggaactgcc
     1921 gggccagctc gcggtgcgag gcgtcgtgct tggccaccac catcaccccg gacgtgcccc
     1981 gatccagccg gtgcacgatg cccggccgca gttcgccgcc gacgccgctc aggtcgtcca
     2041 cgtggtggag gagggcgttc accagcgtac cgctggcgtg tccgggggcc gggtgcacga
     2101 ccatgccggc cgccttgttg acgacgatga cgtcggcatc ctcgaacagc acgtcgaggg
     2161 gcagcgcctc gggctgcggc gcggcgggcg ccgggtctgg cagatccgcc gcgacggcgt
     2221 cgccggcgcg gacggtgtag ttcgccttgc tcccgcgccc gcccacggcg accaggccgt
     2281 ccttgatgag gcgctggacc tgcgaccgcg acggcccgtt cagcttcgag acgaggaaat
     2341 gatcgaggcg ctggcccgcc tcctcgccct cgacccggaa ctcgtagtgg accatggctc
     2401 tccgcagcgg ccgcctcgcc ggtgcccgca tcgccaggtc cccggcccgg ctgcgtgcgc
     2461 cgcgatcagc ggcccctccg cttcctgcgc ggcgcggccg gggccggccc ggccggcgcc
     2521 gccgcgggcc cgcgccgcca cagcaccgcc agcatcgcga tcgacagcgg aaccagaccc
     2581 agcgcgatcc actgcgacgt ggacagcccc atcgcggcgc cacgcggatc gccccggaac
     2641 atctcgatga cgtaccgcga gattccgtag aggaggatgt aagtccacca ggtccacccg
     2701 gcgcgccggc gccagcgcct ctcggtggcg agcaggaaaa gcaggatcag gaactccgcc
     2761 ccggcgtcgt acagctgcgt cgggtggagc ggaacgtgga gcggcgtgcc gacattgctc
     2821 gcggcgaacg gatcggtgaa ggtcacggcc cacgggaccg tcgtcggctt cccgtagcag
     2881 cagccggcca gcaggcatcc gaagcggccg acgacgtgcc cgagggcgag gccaggcgcc
     2941 accgcatcgg ctaccggcca cagcggcagg tggtgcctcc gcacgtacca caggcccacg
     3001 ccgaacgcaa cgatgaggcc gccgtaaaag acgccgcccg tgcgcacgag tgtccagatt
     3061 tcgccggggt gccgcctgaa gtggtcgaag tccacgatca ggagcagcag cttggccccg
     3121 atcagggcgg cgatgatgat gtagacgccc aggtcgagca cgcgggtcgc gtcgaggccg
     3181 aagcggcgcg cccggcggaa ggcgacgtac agcgccagca ggtacgccga cgcgagcagg
     3241 acgccgtagc tgtagaccgt gaacggcccc agctcaagga gtcgagggag catgcctgtt
     3301 caaccccatc atgtccgcga gcatggccgc gaccccgacc gtgatggccg agtcggcgac
     3361 gttgaacgcc cagaagtgcc aggcgccgaa cacgacgtcc acgaagtcca ccaccgagcc
     3421 gaacgcgagg cggtcgatca ggttgccgac ggcgccgccg atgatcaagg cgaggccggc
     3481 acgcgcgagg cgctggcgcg acgacacccg cgccgcgtac agcgcgatcc cgatgagcgc
     3541 cccggtcgcc acgacggcca ccacggccgc cttgccgggg aagtcgacgt cgttcaggaa
     3601 cccgaaggcc gcgcccgtgt tgcgcacgtg cacgaggttc agcacgccgg gcacgacggg
     3661 cacgctgtcg tgcagcggga tctcccgcct caccagcgcc ttggtcagct ggtcgaggag
     3721 gatgacggcc gccgccagcg ccgcctccag ccggacggcc ctgcggcagg ccggccccgc
     3781 gtccgccgcg gcgctcatcg cgcggccgcc agcacggcct cgcagcgcgg gcagacgcct
     3841 tcgcgctccg ggtcgctcga cacggcgggc acgtagcgcc agcagcgctc gcacttcacg
     3901 ccggcggccc gctccgcgac gatgtgcacg gcgctgtgct cgtcttcgcg gtagatcgcg
     3961 ccggcctccg gctcggcgcc ggccgagggg gcgtccgtct cgccgtccac ctgcgacgtg
     4021 atgaacaagc cgggcaactc gtcccggtag gaggcgatga ggtcggcgag ggccccggtg
     4081 ccgcgcaggt gcaccgcggc ctcgagcgac tgcccgatgg ccttcgcttg ccggagccgt
     4141 tccagttccg cattgactgc ggcccggagc ttcagcagcc gcgaccagcg cgccacgagc
     4201 tccgcgtcgc ggaacagctc cacccgcgtc gggaactcgg ccaggtgcac cgattcctcg
     4261 cgctcgccgg gcaggctggc ccagtactcg tcggccagga ccggcaggat gggggcgatg
     4321 agccgcgcca ggccgtctgc gatggcgaac atcgcggtct gcgcggatcg ccgcccgcgc
     4381 gagccggggg ccagggtgta gaggcggtcc ttggagacgt ccacgtagaa cgcgctcagg
     4441 tccaccgcaa cgaaggcgt
//
LOCUS       DAOSIF010000238         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17345_length_4441_cov_3.675787, whole genome shotgun sequence.
ACCESSION   DAOSIF010000238 DAOSIF010000000
VERSION     DAOSIF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17345_length_4441_cov_3.675787"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>852
                     /gene="pyk"
                     /locus_tag="PLT35_08610"
     CDS             <1..>852
                     /gene="pyk"
                     /locus_tag="PLT35_08610"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG29328.1"
                     /translation="ATIGPASDPPEVLQRLILAGMNVARLNFSHGAFEEHARRIGAIR
                     AAERATGRRVAIMADLPGPKIRVGPIAPEPVDLRPGDRLTLTASQEPGTPRRVSVSFE
                     RLPQVVKPGDRLFLNDGLVQLRVDRVEAPDVHCIVAVGGELRSRKGLNLPGIDLGISA
                     FTGRDRECLAFALAHGVDAVSQSFVENAADVVRVREAAHALGHRPFVIAKIERAGALA
                     HADGILAAADGVMVARGDLGVEVPIEQIAMTQKSLIARATLAGKPVITATQMLESMVA
                     SRIPTRA"
     assembly_gap    853..862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <863..1415
                     /locus_tag="PLT35_08615"
     CDS             <863..1415
                     /locus_tag="PLT35_08615"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG29329.1"
                     /translation="AEATDVANAILDGTDAVMLSAESAMGRYPEEAVAMLAKIAAATE
                     PKRPRTSLAALREFCLTHRPASTPEAIARVVETALETVPCGAVFVPARTGTTARMVSR
                     HKPAVWIVAFSRDRAVCQGLAFSYGVWPEHLAAEPDDWRGQAASWMRDRGVRAASAML
                     VAGPSGAHPGANHRIEFMQLGED"
     gene            1415..1984
                     /gene="pnuC"
                     /locus_tag="PLT35_08620"
     CDS             1415..1984
                     /gene="pnuC"
                     /locus_tag="PLT35_08620"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOG29330.1"
                     /translation="MNPFEVAGVASGLLGVWLMTRQKIWAWPVLLFSSLVFIVVFFRA
                     RLYAAMSLQVVYIGLASYGWHQWLHGGGGHGRLRVSRVPRRVALALAGAGAALTAALG
                     TWLDLRTDQALPYADAFTTSFSLAAQFMQARKYLENWAVWLAVDFVYIGMLRSQDLDL
                     TSLLYLVYLVLAILGLRDWRRSMAAPTLP"
     gene            1981..2568
                     /locus_tag="PLT35_08625"
     CDS             1981..2568
                     /locus_tag="PLT35_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29331.1"
                     /translation="MTPPAPFVIVVTGSECTGKTTLAAELAARLGAPCSEEFARRYVG
                     RKGAPLGASDVEAIARGQIAGEDAALAAAARVVVKDTDLVSTVVYARHYYGCCPAWIE
                     RAARERAGDLYLLLHPDVPWVADGPYRDRPAARAELHGLFEAALAEFGAKAIDILGDW
                     GARRARALAALRAWGQIQDSGASSGSGPTPCIPRG"
     gene            complement(2627..>4441)
                     /locus_tag="PLT35_08630"
     CDS             complement(2627..>4441)
                     /locus_tag="PLT35_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29332.1"
                     /translation="NGPDDDPVSPAEYQARLAKASLYIRTVLGAPDVLCVQEAENLGV
                     LEALAARIAADDPSMVYSAHLLEGHDIGGIDNGFLVRDTVAVDSVTQVGYDTRLSLDG
                     SYLNDRPPLVLEGRYLANGAPFPITVIGVHGRSLSGIDGPSANRVRQKRLEQSLELAS
                     YIQGLQVADPARRIVVTGDFNAYQFSDGYVDVVGLVSGSLDGNGALQPGHEDLVQPDL
                     YNVLHTLPEAERYSFVYGGSAQALDHALISASLGAFLREAAFARGNADAPASLLFDTS
                     TAAGTSDHDGLVLFVMTDFDGDGVPDDEEGPAPVGADFNGDATSDILWRGAAGDLQVW
                     GMSGGVKSLDGYVGAVADPDWEIKGQGDLDGDGTVDLLWRHKTDGTIYYWRFEGYTPV
                     EELFVATVDPSYDIVGTGDFDGNGTSDILWRGAGGDLWMWTMDGASVTGEFPLGVVDA
                     GYRVEGIGDLDADAKADIAWRGAAGDVWVWLMNGPVKRKEAYLATVDLAYEIQQVADF
                     DGLGAADLLWWNATLGDVWIWFMNGTAVVSEACVGIGVDTDYRIVGAGDYNGDGKADI
                     LWHRAAEGDVWVWVMNGSVRASEIFVDLAAGPGYQVVK"
BASE COUNT          587 a         1662 c         1506 g          676 t
ORIGIN      
        1 tcgccaccat cggcccggcc tcggacccgc cggaggtgct gcagcggctc atacttgccg
       61 gcatgaacgt ggcgcggctc aatttttcgc acggggcctt cgaggagcac gcgcggcgga
      121 tcggcgcgat ccgcgccgcc gagcgcgcga cgggccggcg cgtggcgatc atggccgatc
      181 tgccggggcc gaaaatacgt gtcggcccga tcgctcccga gccggtggac ctgcgcccgg
      241 gcgaccgcct caccctcacc gccagccagg agccgggcac gccccgccgc gtgtccgtgt
      301 ccttcgagcg cctgccgcag gtcgtcaagc cgggggaccg cctgttcctc aacgacggcc
      361 tcgtgcagct ccgcgtcgat cgcgtcgagg ccccggacgt ccactgcatc gtggccgtcg
      421 gcggcgagct gcgatcgcgg aagggcctga accttcccgg catcgatctc ggcatctccg
      481 ccttcaccgg gcgcgatcgc gagtgcctcg ccttcgccct cgcgcacggc gtcgacgccg
      541 tcagccagtc gttcgtcgag aacgcggccg acgttgtccg cgtccgcgag gcggcgcacg
      601 ccctggggca ccggccgttc gtcatcgcga agatcgagcg cgctggcgcg ctggcgcacg
      661 ccgacgggat cctcgcggcc gccgacggcg tgatggtggc ccggggcgac ctgggcgtcg
      721 aagtccccat cgagcagatc gcgatgacgc agaagtcgct gatcgcgagg gccacgctgg
      781 cgggaaagcc cgtcatcacg gcgacccaga tgctggagtc gatggtcgcg agccgcatcc
      841 ccactcgcgc cgnnnnnnnn nncgccgagg cgaccgacgt cgccaacgcg atcctggacg
      901 gcaccgacgc ggtcatgctg tcggccgaat cggccatggg gcggtatccg gaggaggccg
      961 tcgccatgct ggcgaagatc gcggcggcca ccgagccgaa gcggcctcgc acgtccctcg
     1021 ccgcgctccg cgagttctgc ctgacgcacc gcccggccag cacgccggag gcgatcgcca
     1081 gggtcgtgga gaccgccctg gagacggtgc cgtgcggcgc ggtgttcgtg ccggcgcgca
     1141 ccggcacgac ggcgcgcatg gtgtcccgcc acaagccggc cgtctggatc gtcgccttca
     1201 gccgggaccg cgcggtctgc caggggctcg ccttctccta cggcgtctgg ccggagcatc
     1261 tcgcggccga gcccgacgac tggcgcggcc aggcggcctc ctggatgcgc gatcgcggag
     1321 tgcgcgcggc gtcggcgatg ctcgtcgcgg ggccgtcggg cgcgcatccc ggcgccaacc
     1381 accgcatcga gttcatgcag ctcggggagg attgatgaat ccgttcgagg tcgccggagt
     1441 cgcctccgga ctgctgggcg tgtggctcat gacgcggcag aagatctggg cctggcccgt
     1501 cctgctgttc tcgtccctgg tcttcatcgt cgtgttcttc cgggccaggc tctacgccgc
     1561 gatgagcctg caggtggtgt acatcggcct cgcctcctac ggctggcacc agtggctgca
     1621 cgggggcggc ggccacgggc ggctccgcgt gtcgcgcgtg ccgcgacgcg tcgccctcgc
     1681 gctggccggg gctggcgcgg cgctcacggc cgcgctgggc acgtggctgg atctgcgcac
     1741 cgaccaggcg ctcccgtacg ccgacgcctt caccacgtct ttcagcctcg ccgcgcagtt
     1801 catgcaggcg cggaagtacc tcgagaactg ggccgtatgg ctcgccgtgg acttcgtcta
     1861 tatcggcatg ctgaggagcc aggacctgga cctgacctcc ctgctctacc tcgtctacct
     1921 ggtgctggcg atcctcggct tgcgcgactg gcgccgctcg atggccgccc cgacgctgcc
     1981 gtgacgcccc ccgcgccgtt cgtcatcgtc gtcacaggct cggagtgcac cggcaagacg
     2041 acgctggcgg cggagctggc ggcgcggctc ggggcgccct gctcggagga gttcgcgcgc
     2101 cggtacgtcg gccgcaaggg cgcgcccctc ggcgcctcgg atgtcgaggc catcgcgcgc
     2161 gggcagatcg cgggcgagga cgccgccctc gccgcggccg cgcgggtcgt cgtgaaggac
     2221 accgatctcg tcagcacggt ggtgtacgcg cgccactact acgggtgctg ccccgcgtgg
     2281 atcgagcggg cggcgcgcga gcgcgccggc gacctctacc tgctgctgca cccggacgtg
     2341 ccgtgggtag ccgacggccc gtatcgcgac cggccggccg cccgcgccga gctgcacggc
     2401 ctgttcgagg cggcgctcgc cgagttcggc gcgaaggcga tcgacattct cggcgactgg
     2461 ggcgcccggc gcgcccgcgc cctcgccgcc ctccgcgcct ggggtcagat ccaagattcc
     2521 ggtgcctcgt cgggatccgg ccccaccccc tgcatccccc ggggctgacg ccccgtggct
     2581 tcgtttgcct tccggatttc gaagtccgga ttccgggtcc ctgcccctac ttcacgacct
     2641 gataccccgg acccgccgcc aagtcgacga agatctccga cgccctcacc gacccgttca
     2701 tcacccacac ccacacgtcg ccctcagcgg cacggtgcca caggatgtcg gccttgccgt
     2761 ccccgttgta gtcgcccgca cccacgatcc ggtagtccgt gtcaacgccg atcccgacgc
     2821 acgcctcgga caccaccgcc gttccgttca tgaaccagat ccagacgtcg cccagcgtcg
     2881 cgttccacca cagcaggtcc gccgcgccga ggccgtcgaa gtccgcgacc tgctgtatct
     2941 cgtacgccag atccaccgtc gccaggtagg cttccttgcg cttcaccggg ccgttcatca
     3001 gccacaccca cacgtcgccc gccgcgccgc gccaggcgat gtcggccttc gcgtcggcat
     3061 ccaggtcgcc gatgccttca acccggtaac cggcgtccac cacgccaagc gggaactccc
     3121 ccgtgacgct ggcgccgtcc atcgtccaca tccacaaatc gccgcccgcg ccgcgccaca
     3181 ggatgtcgga cgttccgttc ccgtcgaagt cgcccgtccc gacgatgtcg tacgagggat
     3241 cgaccgtcgc cacgaacagc tcctccaccg gggtgtagcc ctcgaatcgc cagtagtaga
     3301 tcgtgccgtc ggtcttgtgg cgccagagca ggtcgaccgt cccgtcgccg tccaggtcgc
     3361 cctggccctt gatctcccag tccgggtcgg cgactgcgcc gacgtatccg tcgagcgact
     3421 tgacgccgcc cgacatgccc cagacctgca gatcgccggc tgccccgcgc cacaggatgt
     3481 ccgaggtcgc atcgccgttg aagtcggcgc caacgggcgc cggtccctct tcgtcgtcgg
     3541 gcacgccgtc gccgtcgaag tcggtcatca cgaagagcac cagcccgtcg tgatcggacg
     3601 tgccggccgc ggtcgacgtg tcgaacagca gcgaagccgg cgcgtcggcg ttgccgcgcg
     3661 cgaacgccgc ctcgcgcagg aacgcgccca ggctcgccga gatcagcgcg tggtcgagcg
     3721 cctgcgcgct gccgccgtag acgaacgagt agcgctcggc ctccggcagc gtgtgcagga
     3781 cgttgtagag atcgggctgg acgagatcct cgtgtccggg ctgcagcgcg ccgtttccgt
     3841 cgaggctgcc gctgacgagc ccgacgacgt cgacgtagcc gtcgctgaac tggtaggcgt
     3901 tgaagtcgcc cgtgacgaca atccgccggg ccggatcggc gacctgcagg ccctggatgt
     3961 aggacgccag ttcgagcgac tgttcgagcc gcttctgccg cacgcggttg gcgctggggc
     4021 cgtcgatgcc gctgagcgac cggccgtgca cgccaatcac cgtgatgggg aacggcgcgc
     4081 cgttggcgag gtagcgcccc tcgagcacca ggggcggccg gtcgttgagg tagctcccgt
     4141 cgaggctcag ccgcgtgtcg tacccgacct gcgtgacgct gtcgacggcc accgtgtctc
     4201 tcaccaggaa cccgttgtcg atgccgccga tgtcgtggcc ttcgaggaga tgcgcgctgt
     4261 acaccatcga cgggtcgtcg gccgcgatcc gcgcggccag cgcctccagc acgccgaggt
     4321 tctcggcctc ctgcacgcac agcacgtcgg gcgcgccaag aacggtccgg atgtagagcg
     4381 acgccttcgc gagcctcgcc tggtactccg ccggcgacac cgggtcgtcg tccggcccgt
     4441 t
//
LOCUS       DAOSIF010000239         4401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17543_length_4401_cov_4.178325, whole genome shotgun sequence.
ACCESSION   DAOSIF010000239 DAOSIF010000000
VERSION     DAOSIF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17543_length_4401_cov_4.178325"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLT35_08635"
     CDS             complement(<1..248)
                     /locus_tag="PLT35_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29333.1"
                     /translation="MPQIELTELTLPELEDAFAQLGLERYRARQVFQWVYRRGACDFD
                     LMTSLPAAHRRLLAARFRVSTPSIARRDVSADGTEKFLL"
     gene            385..1647
                     /locus_tag="PLT35_08640"
     CDS             385..1647
                     /locus_tag="PLT35_08640"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG29334.1"
                     /translation="MIVREVLDNGLRLLSEPLPHVRSVSLGVWLARGSRHEGDASPGI
                     AHFVEHMLFKGTTTRTAEEIAQAVDSVGGQMDAATGKENANYGIKVLDEHLGLAVDVL
                     SDVVLHPKFAPADIEREKSVVAEEIKMVEDMPDDLVHELFIERFWAGHPLGRSILGTP
                     DSVEALTREALQAYFSRVHTASNFVVAAAGSFDYERLRDLVQEAFGSLPRGGGEPEDA
                     PPSAHGVLLVRTKDLEQSHVCLGAGAYPQNHASRYAALVFNTLLGGSMSSRLFQNVRE
                     KRGLAYSVSSGLSAFRDAGLLTIYAGCANTKVDEVVRVIMDELRAAVAQPVAASDLAR
                     AKDNVKGGLILGLESTWQRAANLARQELYFGEPFTLDETLAGIDAVTADDVARVAQEL
                     LAGAPLSLAVVGAVDGWTITEEDLQVTR"
     gene            1644..2948
                     /gene="purB"
                     /locus_tag="PLT35_08645"
     CDS             1644..2948
                     /gene="purB"
                     /locus_tag="PLT35_08645"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOG29335.1"
                     /translation="MIARYTHPEMGGIWSDDRRYRTWLAVEIAAAEAMADRGIIPAEA
                     ARAIRERGAVDAARVEAIEAVTKHDLVAFTTAVAERVGPEARWLHFGLTSSDVVDTAF
                     AVQLRDACALILRRLDALRASVRARAEEHRHTPMIGRTHGVHAEPTTFGLKLALWYAE
                     LGRDAARVERGRDAVSVGKISGAVGTYAHLEPAVERAVCAKLGLEPAPVSSQVVQRDR
                     HAELMAALAITAASLEKFALEIRGLQKTEIGEVEEPFDQGQKGSSAMPHKRNPVGCEQ
                     IAGLARVIRGNLGAALENIALWHERDISHSSVERIIFPDSFIALDHMLRRFTRIVSGM
                     AVHPDRMRRNLELLRGVVFSGPLLLALAARGVPRERAYEWVQRNALRSADEGLDFRGL
                     LRADADVAGALPAEEMDRVFDLGHQLRHADEIFARVFGGGRA"
     gene            2976..3182
                     /locus_tag="PLT35_08650"
     CDS             2976..3182
                     /locus_tag="PLT35_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29336.1"
                     /translation="MDERPGHRRWIVMAAFASGAALLVLAALAFAGVLAFRDPARLVA
                     GAAFAAAGVGDLLAGFVLLRSHAG"
     gene            3172..>4401
                     /gene="purF"
                     /locus_tag="PLT35_08655"
     CDS             3172..>4401
                     /gene="purF"
                     /locus_tag="PLT35_08655"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG29337.1"
                     /translation="MQDKFNDECGVFGIYGQAEAAKLTYLGLYALQHRGQESGGIAAC
                     DAGRLRAFRGMGYVADVFDERVLADLPGPAAIGHVRYSTAGTSGLSNAQPILIECVHG
                     PIAVCHNGSLVNERELRRELSAQGAIFQTSSDTEVFLHLYARSKAASLEDALVEAVGQ
                     AQGAFSLLFLAPGRLVAVRDANGFRPLALGRLGEAWVVCSETCAMDLIGATYVRDVEP
                     GEMLVIGPDGPRSLRPFPPAPRAHCIFEHVYFARPDSLVFGESVNRVRTNLGRVLARE
                     SGVPADVVVPVPDSGVCAAVGYAERSGIPIQFGLIRNHYVGRTFIEPQQAIRHFGVKV
                     KLNPVRSILEGRRVVLVDDSIVRGTTSRKLVKMIKAAGALEVHVRISCPPTIAPCFYG
                     VDTPRRAELIAATHTLEE"
BASE COUNT          598 a         1509 c         1666 g          628 t
ORIGIN      
        1 aggaggaact tctcggtgcc gtccgcggac acgtccctcc gcgcgatcga cggcgtcgac
       61 acgcggaagc gcgcggcgag caggcgccgg tgcgccgccg ggaggctggt catcaggtcg
      121 aagtcgcacg cgccgcgccg gtagacccac tggaacacct gcctagcccg gtagcgctcg
      181 aggccgagct gcgcgaaggc gtcctcgagc tcggggagcg tgagttcggt caactcgatc
      241 tgcggcatgg ggcgggctcg tttctttcgt tgctgcggcg atcgggccca tgctatcatc
      301 gtgaggttgg ctcggccaca attcctttat gtacagtcat ttgcggcgac gcgccaactc
      361 caggcccaag gggatcgccc gccgatgatc gtgcgcgagg ttctcgacaa cggactgagg
      421 ctcctgagcg agccgctgcc gcacgtgcgc tcggtgagcc tgggcgtctg gctggcgagg
      481 ggctcgcggc acgagggcga cgcctcgccc ggcatcgcgc acttcgtcga gcacatgctg
      541 ttcaagggga ccacgacccg gacggcggag gagatcgcgc aggcggtgga ctcggtcggg
      601 gggcagatgg acgccgcgac gggcaaggag aacgcgaact acgggatcaa ggtgctggac
      661 gagcacctgg ggctggcggt ggacgtgctg tcggacgtcg tgctgcaccc gaagttcgcc
      721 cccgccgaca tcgagcgcga gaagagcgtc gtcgccgaag agatcaagat ggtcgaggac
      781 atgcccgacg atctcgtcca cgagctgttc atcgagcggt tctgggcggg gcaccccctc
      841 ggccggtcga tcctgggcac gcccgacagc gtggaggcgc tgacgcggga ggcgctccag
      901 gcgtacttct cccgggtcca cacggcgtcg aacttcgtcg tggcggcggc cgggagcttc
      961 gactacgaac ggctgcggga cctcgtgcag gaggcgttcg gaagcctgcc ccgcggcggc
     1021 ggcgagccgg aggacgcgcc gccctcggcg catggcgtcc tgctcgtgcg gaccaaggac
     1081 ctcgagcaga gccacgtttg cctgggcgcg ggggcgtacc cccagaacca cgcgagccgg
     1141 tacgccgcgc tcgtcttcaa cacgctgctc ggcgggtcga tgagctcgcg gctgttccag
     1201 aacgtgcggg agaagcgcgg cctggcgtat tcggtgagca gcgggctgag cgcgttccgc
     1261 gacgcggggc tgctcaccat ctacgcgggc tgcgcgaaca cgaaggtgga cgaggtggtg
     1321 cgggtgatca tggacgagct gcgcgcggcg gtggcgcagc cggtggccgc ctccgatctg
     1381 gcccgggcga aggacaacgt gaagggcggg ctgatcctgg gcctcgagag cacctggcag
     1441 cgcgccgcga acctcgcccg ccaggagctg tacttcggcg agccgttcac gctggacgag
     1501 acgctggcgg gcatcgacgc cgtcacggcg gacgacgtcg cgcgcgtggc gcaggagctt
     1561 ctggcggggg cgccgctctc gctggcggtg gtcggcgcgg tggacggctg gacgatcacg
     1621 gaagaggatc tgcaggtgac gcgatgattg cgcgctacac gcaccccgag atgggcggga
     1681 tctggagcga cgaccggcgc taccggacgt ggctggcggt ggagatcgcg gcggccgagg
     1741 cgatggccga tcggggcatc atcccggcgg aggcggcgcg ggcgatccgg gagcgcggcg
     1801 cggtggacgc ggcgcgcgtg gaggcgatcg aggcggtcac gaagcacgac ctcgtcgcgt
     1861 tcacgacggc cgtggccgag cgcgtcgggc cggaggcgcg ctggctgcac ttcggcctga
     1921 cgtcctccga cgtggtggac accgccttcg cggtgcagct gcgggacgcc tgcgcgctga
     1981 tcctccggcg gctcgacgcc ctgcgcgcct cggtgcgcgc gcgggcggaa gagcaccggc
     2041 acacgccgat gatcggccgg acgcacggcg tgcacgccga gccgacgacc ttcggcctga
     2101 agctggcgct gtggtacgcc gagctcgggc gcgacgcggc gcgcgtcgag cgcgggcgcg
     2161 acgccgtgtc ggtgggcaag atctcgggcg ccgtcggcac ctacgcgcac ctcgagccgg
     2221 ccgtcgagcg ggcggtgtgc gcgaagctgg gcctggagcc ggcgccggtg tcgtcgcaag
     2281 tcgtccagcg cgaccgccac gcggagctga tggcggcgct cgccatcacg gcggcctcgc
     2341 tggagaagtt cgccctcgag atccgcggcc tccagaagac ggagatcggg gaggtcgagg
     2401 agcccttcga ccagggccag aagggctcgt cggcgatgcc ccacaagcgc aaccccgtcg
     2461 ggtgcgagca gatcgcaggc ctggcgcgcg tcatccgggg caacctcggc gccgccctcg
     2521 agaacatcgc gctctggcac gagcgcgaca tctcgcactc atcggtcgag cggatcatct
     2581 ttcccgacag cttcatcgcg ctcgatcaca tgctccggcg cttcacgcgc atcgtctcgg
     2641 ggatggcggt ccaccccgat cgcatgcggc gcaacctgga gctgctgcgc ggcgtcgtgt
     2701 tctcggggcc gctgctcctg gcgctggccg cgcgcggcgt gccccgcgag cgggcgtacg
     2761 agtgggtgca gcgcaacgcg ctgcgatcgg ccgacgaggg cctggacttc cgcggcctgc
     2821 tgcgcgcgga cgcggacgtg gcgggcgcgc tgccggccga ggagatggat cgcgtgttcg
     2881 atctcggcca ccagctccgc cacgcggacg agatcttcgc ccgcgtgttc ggcggcggcc
     2941 gggcctgatt gggccccgcg ggggagggcg aggcgatgga cgagcggccc ggccaccggc
     3001 gctggatcgt gatggcggcg ttcgcttccg gcgcggcgct gctcgtcctg gcggccctcg
     3061 ccttcgcggg cgtcctggcg ttccgggacc cggcgcgcct cgtcgcgggc gcggcgttcg
     3121 ccgccgcggg cgtcggcgac ctgctcgcgg gcttcgtgct cctccggtcc catgcaggat
     3181 aagttcaacg acgagtgcgg cgtcttcggg atctacgggc aggccgaggc ggccaagctc
     3241 acgtacctcg ggctgtacgc cctgcagcac cgcggccagg agagcggcgg gatcgccgcc
     3301 tgcgacgccg ggcgcctccg cgcgttccgc gggatggggt acgtcgccga cgttttcgac
     3361 gagcgcgtgc tcgcggacct gccggggccc gccgccatcg gccacgtgcg ctactcgacg
     3421 gcgggcacga gcggcctgtc gaacgcgcag ccgatcctca tcgagtgcgt gcacggcccc
     3481 atcgccgtct gccacaacgg gagcctcgtc aacgagcgcg agctgaggcg ggagctgtcg
     3541 gcgcaggggg cgatcttcca gacctcgagc gacaccgagg tgttcctgca cctgtacgcc
     3601 cggtcgaaag cggcctccct cgaagacgcc ctcgtcgagg ccgtgggcca ggcgcagggg
     3661 gcgttctcgc tgctgttcct ggcgccgggc cgcctggtgg ccgtgcgcga cgccaacggc
     3721 ttccggcccc tcgcgctcgg ccgtctggga gaggcgtggg tcgtctgctc cgagacgtgc
     3781 gccatggacc tcatcggcgc gacctacgtg cgcgacgtcg agccgggcga gatgctcgtc
     3841 atcggccccg atgggccgcg gtcgctccgg ccgtttccgc cggcgccgcg cgcgcactgc
     3901 atcttcgagc acgtgtactt cgccaggccc gacagcctcg tcttcggcga gagcgtcaac
     3961 cgcgtgcgga ccaatctcgg gcgcgtgctc gcgcgcgagt ccggcgtgcc ggccgacgtc
     4021 gtcgtccccg tgccagactc cggcgtgtgc gccgccgtcg ggtacgcgga gcggtccggc
     4081 atcccgatcc agttcggcct gatccgcaac cactacgtcg ggcgtacctt catcgagccg
     4141 cagcaggcga tccgccactt cggcgtgaag gtgaagctca accccgtgcg cagcatcctc
     4201 gagggcaggc gcgtggtgct cgtcgacgac tcgatcgtgc gcgggacgac gagccggaag
     4261 ctggtgaaga tgatcaaggc cgccggcgcc ctcgaggtcc acgtgcgcat cagctgcccg
     4321 ccgaccatcg cgccgtgctt ctacggggtg gacacgccgc gccgggccga gctcatcgcc
     4381 gccacgcaca ccctcgagga g
//
LOCUS       DAOSIF010000240         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17567_length_4398_cov_3.374396, whole genome shotgun sequence.
ACCESSION   DAOSIF010000240 DAOSIF010000000
VERSION     DAOSIF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17567_length_4398_cov_3.374396"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLT35_08660"
     CDS             <1..382
                     /locus_tag="PLT35_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29338.1"
                     /translation="ITAGRDLVGALLMGHPFNSWWTGSILSIAQARRRVRDVNATALQ
                     VAAGVLAAVLWIERNPNRGLCFPEDLPHEEILRFARPYLGRIVSVRSDWTPLQRHRVY
                     FSENPEARANHEDVWQLRNFLFSP"
     gene            complement(520..1197)
                     /locus_tag="PLT35_08665"
     CDS             complement(520..1197)
                     /locus_tag="PLT35_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOG29339.1"
                     /translation="MVMRKTIGIAIVALAMALAAAPAQADLIYYTGTDANNDGIDDAF
                     TVNGNPAYLVTKTANGWPVLPNPSITLGKYISWAADQSNATQGSQTGQLFTYAFSFNW
                     AGLPLTTTFDFRWLSDDYLSDVLLNGTSLGVNNLGQPSPWTQSYAVNGVSGTVQSGIN
                     TIEFLIWNTGGQQPPYYGQSGPTGMAADFTVHGEATTVPEPASSMALLAMGLAGLACW
                     RARQQSR"
     gene            complement(1532..1607)
                     /locus_tag="PLT35_08670"
     tRNA            complement(1532..1607)
                     /locus_tag="PLT35_08670"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1572..1574),aa:Phe,seq:gaa)
     gene            complement(1800..2867)
                     /gene="recA"
                     /locus_tag="PLT35_08675"
     CDS             complement(1800..2867)
                     /gene="recA"
                     /locus_tag="PLT35_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOG29340.1"
                     /translation="MPVDDRTERTKALDVALSQIEKQFGKGSIMRLGQKGAILPVDSI
                     PTGAVSLDFALGIGGMPRGRVVEIYGPESSGKTTLALQLIAEAQKLGGMAAFVDAEHA
                     LDATYAQKLGVDLDNLLVSQPDNGEQALEIVEVLVRSGGVDVVVVDSVAALVPRAEIE
                     GEMGEAQMGLQARLMSQALRKLTGVVSKSKTSLVFINQLREKIGVMFGSPETTTGGRA
                     LKFYASVRLDIRRIASIKDGEMVVGGRVRVKVVKNKVAPPFREAEFDILYGEGISKEG
                     DLLDLAVDRKIVEKSGTWFSFSGERLGQGRENAKQFLRENRGVCKAIEERVRKELGLV
                     REASSEAEPASEKEKASPSRR"
     gene            3075..3662
                     /locus_tag="PLT35_08680"
     CDS             3075..3662
                     /locus_tag="PLT35_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOG29341.1"
                     /translation="MPSPIGHMVAGLAVGWAAHALEAARSQPGDPAGAAPWRLASAGS
                     IPVAFCALLAAAPDADILMASHRTYTHSVGAIALVGLAAAAGALWLRRPAVTAALVCA
                     AAWGSHVFLDWLGHDGTPPIGLMALWPFTDRYYSSGFDLFADVSRRYWNLDEFVFGNA
                     RSIARELVVLAPLALAALWWQRRSTGPPPHPAPGL"
     gene            complement(3685..>4398)
                     /locus_tag="PLT35_08685"
     CDS             complement(3685..>4398)
                     /locus_tag="PLT35_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOG29342.1"
                     /translation="IERSLAKLVEKGVIDAEASTAALGRIAFTGELSQLAAADLVVEA
                     AVEDLAVKRGLFGALDALVRPGVILASNTSALPIGSLASATTRPALVLGMHFMNPVPL
                     RPLVELVRGARTSPDAMATAAEVCRRLGKTPVESADSPGFIANRLLMPMINEATFAAM
                     EGIGTPEAIDTVMKLGMNHPMGPLALADLIGLDVCLAIMKALHAGFGDPKYRPCPLLE
                     EMVASGRLGRKSGKGFYEY"
BASE COUNT          673 a         1546 c         1428 g          751 t
ORIGIN      
        1 gatcacggcg ggccgggacc tggttggcgc gctgctgatg gggcatccgt tcaactcgtg
       61 gtggaccggc agcatcctct cgatcgcgca ggcccgccgc cgggtgcgcg acgtcaacgc
      121 caccgcgctg caggtggcgg cgggcgtgct cgccgccgtg ctctggatcg agcgcaaccc
      181 gaaccgcgga ctgtgcttcc cggaggacct gccccacgag gaaatcctgc ggttcgcgcg
      241 gccgtacctg gggcggatcg tctccgtacg ctcggattgg acccccctcc agcgtcatcg
      301 cgtctacttc agcgagaacc ccgaggcacg cgctaaccac gaggacgtgt ggcagcttcg
      361 gaacttcttg ttctccccct gattcacgcc gcgtcaggag cggcgcgccg gcggcgccgg
      421 cgcgagggag gcccggcagt gcagggcctt gagcgggccg tacgcagcga gggccacctc
      481 ccagggtggc cctcttcgct ggcgcctagc ttcgacgaat caccgggact gctgccgcgc
      541 acgccagcac gccagccccg cgagccccat ggccaggagc gccatcgatg acgccggctc
      601 gggaacggtc gtggcttcgc cgtgcaccgt gaaatccgca gccatgcccg tcggcccgct
      661 ctggccgtag taaggaggct gctggccgcc ggtgttccag atcagaaact cgatggtgtt
      721 gatgccactc tggaccgtgc cggatacacc gttcacggcg tacgactggg tccagggcga
      781 cggctggccg aggttgttca cgccgagact cgtcccattg agaagaacgt cgctgaggta
      841 gtcgtccgag agccagcgga agtcgaacgt cgtggtcagc ggcagtccgg cccagttgaa
      901 agagaaggcg tacgtgaaga gctggcccgt ctggctgccc tgcgtcgcgt tggactggtc
      961 ggccgcccag gagatgtact tgccgagagt aatgctcgga ttcggcagca ccggccagcc
     1021 gttggcggtc ttggtaacca ggtaagccgg gttgccgttc accgtgaagg cgtcgtcgat
     1081 cccgtcgttg ttggcgtccg tgccggtgta gtagatgagg tcggcctgag ccggggcggc
     1141 cgccagggcc atggccaggg cgactatcgc gattccaatc gtcttgcgca ttaccatgct
     1201 cctctcaacg gcgagccggt cgttccggct cgtcgccgtt ctatatagca aattcgatgc
     1261 ccgatggatg accgacggag ggttgggtct atcgtaactc caagtgatgc tggtacagta
     1321 ggataaaggc gttaacccga ctggcccggc cgggccgtca cccacggtac gtcaacttgt
     1381 ccgacgttct gagcctcaat aggcgagggg cctccagcgt gccggcaagt ccaccgcgac
     1441 cccaaggcgc ctggctggtg ccatcagtgc accctcagcg caacccctgc ggccggggtt
     1501 cgccgaaatc atcggcatgc tcaaggaatt gtggtgccga ggggcagagt tgaactgccg
     1561 acaccgtgat tttcagtcac gtgctctacc acctgagcta cctcggcact aaggtcagcc
     1621 gcgtggcgag atggccacta tagcacgcag atcgccgccc gcaaaacacg cgcagccagc
     1681 gcgccgagcc cggcacgcca caacgagcac gcccacggga cccgactgct cgggtcttcg
     1741 cgccgaagcc cggatgaaaa gtccgggctg ctgaggcccg gcgccgatcg tgcgcgcccc
     1801 tacctccgcg atggagacgc cttctccttc tccgaggcgg gctcggcctc ggacgaggcc
     1861 tcgcgcacga gcccgagctc cttccttacg cgctcctcga tcgccttgca gacgccccgg
     1921 ttctcccgga ggaactgctt cgcgttctca cgcccctgcc cgaggcgctc cccgctgaac
     1981 gaaaaccatg tcccgctctt ctccacgatc ttccgatcga ccgccaggtc gagcaggtcg
     2041 ccttccttcg agatgccctc gccgtacagg atgtcgaact ccgcctcgcg gaacggcggc
     2101 gccaccttgt tcttgacgac cttgacccga acccggccgc cgaccaccat ctcgccatcc
     2161 ttgatgctcg cgatgcggcg gatgtcgagg cgcaccgacg cgtagaactt cagcgcgcgc
     2221 ccgcccgtcg tcgtctcggg gctgccgaac atcacgccga tcttctcgcg caactggttg
     2281 atgaacacga gcgacgtctt ggacttcgac acgacgccgg tcagcttgcg cagagcctgc
     2341 gacatcaggc gggcctgcag gcccatctgc gcctcgccca tctccccctc gatctccgcg
     2401 cgcggcacca gcgccgccac cgagtccacg accacgacgt caacgccgcc ggagcgaacg
     2461 agcacctcga cgatttcgag cgcctgctcc ccgttgtccg gctgcgacac cagcaggttg
     2521 tcgagatcga cgccgagctt ctgcgcatag gttgcgtcga gcgcgtgctc cgcgtccacg
     2581 aacgccgcca tgccgcccag cttctgcgct tcggcgatga gctgcagcgc cagcgtcgtc
     2641 ttgcccgacg actcgggacc gtagatttcg acgacgcgcc cgcgcggcat gccgccgatg
     2701 ccgagcgcga agtccaggct gacggcgccc gtgggaatgg aatcaacggg gaggatggcg
     2761 cccttctggc ccaggcgcat gatggacccc ttgccgaact gcttctcaat ctgggaaagc
     2821 gcgacgtcca gcgccttcgt acgttccgta cggtcgtcta cgggcatgac tgctccttgc
     2881 tgtgccggcc ggtcaccgaa gcattctaga tgcgtgccgt cagctcgtca agcgaaaatc
     2941 ggcattcaac gcggctttat atgtttgttt tcaactagtt acgctgcgcg ccgctttcgc
     3001 ccttcgctcc atgccatggc agaaagcgcc tggcgccctt gtcggagcag ggcagagact
     3061 gctagggttg ggtaatgcca agccccatcg gccatatggt ggccggactc gccgtcggct
     3121 gggccgcgca cgcgctcgaa gccgcccgct cacagccggg agacccggcc ggcgcagcgc
     3181 cctggcggct cgccagcgcc ggctcgatcc cggtcgcctt ctgcgccctg ctggcagccg
     3241 cacccgatgc cgacatcctg atggcgtctc accgcaccta cactcacagc gtcggcgcca
     3301 tcgcccttgt gggactggcg gccgccgccg gcgcgctgtg gctgcggcgc ccggccgtca
     3361 cggccgcgct cgtctgcgcg gcggcgtggg gcagccacgt cttcctcgac tggctcggcc
     3421 acgatggcac gcccccgatc ggcctgatgg cgctctggcc gttcaccgac cggtactact
     3481 cctcgggttt cgacctgttc gccgacgtgt cgcggcgcta ctggaatctc gacgagttcg
     3541 tcttcggcaa tgcgcgttcc atcgcgcgcg agctggtggt gcttgcgccg ctggcgcttg
     3601 ctgcgctctg gtggcaaagg cggtcgaccg gtccgccacc gcacccggcg cccggcctct
     3661 gatcgccggt cccggctccc gttgtcaata ctcgtagaac cccttcccgc tcttccgccc
     3721 aagccggcct gacgccacca tctcctcgag cagggggcag ggccggtact tcggatcgcc
     3781 gaatccggcg tgcagcgctt tcatgatggc gaggcacacg tcgagcccga tgaggtcggc
     3841 gagcgcgagc ggccccatcg gatggttcat gccgagcttc atgacggtgt cgatcgcctc
     3901 cggcgtgcca atgccttcca tggcggcgaa ggtcgcctcg ttgatcatcg gcatgagcag
     3961 ccggttggcg atgaagccgg gcgagtcggc cgactcgacg ggcgtcttgc ccagccgccg
     4021 gcatacctcg gcggcggtcg ccatggcgtc gggcgatgtg cgcgccccgc gcacgagctc
     4081 caccagcggc ctcagcggca cggggttcat gaagtgcatg cccagcacga gcgccgggcg
     4141 agtcgtggcg gaggcgaggc ttccgatcgg gagcgcggag gtgttcgacg ccagaatgac
     4201 gccggggcgg acgagagcgt ccagcgcgcc gaagaggccg cgtttgacgg ccaggtcctc
     4261 gacggccgct tcgacaacga gatcggcggc ggccagctgg gacagctcgc cggtgaaggc
     4321 gatccggccg agcgcggccg tgctcgcctc ggcgtcgatc acgcccttct cgaccagctt
     4381 cgcgaggctc ctttcgat
//
LOCUS       DAOSIF010000241         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17606_length_4388_cov_4.078698, whole genome shotgun sequence.
ACCESSION   DAOSIF010000241 DAOSIF010000000
VERSION     DAOSIF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17606_length_4388_cov_4.078698"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLT35_08690"
     CDS             <1..500
                     /locus_tag="PLT35_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOG29343.1"
                     /translation="VCTAGAMYLRAWVPERSQVLTFAAHRVRRVSVLEERFVPDPEWA
                     ERALSQSLGPTSGPPVHIVLRFDRALAPMVAERQYHPSQVVTRHPDGSLTLELDVCDD
                     VWLRSFVLQFGHRVRVIGPPTLAEQIRDELDAARRHYAPADRVEALATSQALIDLGDQ
                     WRLPF"
     gene            complement(576..914)
                     /locus_tag="PLT35_08695"
     CDS             complement(576..914)
                     /locus_tag="PLT35_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29344.1"
                     /translation="MIHGNRDARRLWQGLDEAAPGARGRDKTVRPRARFCAACRIRRA
                     LFARCGEVKADRQHTLCFQCYRAERNRLRAGRLSAAGAGVTDPARPAGRAAPDRAPLM
                     ADIELRRLQA"
     gene            1095..2126
                     /locus_tag="PLT35_08700"
     CDS             1095..2126
                     /locus_tag="PLT35_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOG29345.1"
                     /translation="MRAQVLRRIAPVDSDRDSLELCELPDPIPGPGDVLIRVSACGVC
                     HTELDEIEGRTPPPLLPVVPGHQVVGRVVQCGPRPSVLKPGTRVGVAWIYSACGRCVY
                     CLAGQENLCASFRATGRDVNGGYAELLVARQDFVFPLPDTPLTDAEAAPLLCAGAIGW
                     RSLRLANLPNGGRLGLTGFGASAHLVLKLVRHRDPEAEVFVFARSEAERSFARELGAA
                     WTGDTADAAPWPLDAIIDTTPAWRPVVGALKNLAPGGRLVINAIRKEHGDRAALAEID
                     YPRDLWMEKEVKSVANVTRRDVTEFLLAAVAMGLRPEIAEYPLSGANEALRHLKARAA
                     RGARVLRLD"
     gene            2273..2908
                     /locus_tag="PLT35_08705"
     CDS             2273..2908
                     /locus_tag="PLT35_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29346.1"
                     /translation="MRNGKLALALLAALVLAPASAQAQYAAQEALGDPDVCITGYYNL
                     NFGGDVYKGLSATTWHASRSHGLGASVTLLARHLVSAEVDFNYSHSFFGTSDSLNKNY
                     VLTTTLDGVVGPWFSLGSGRIRTYAVAGGGYMRGMITNRRRAGWSHPSKNLGVVEAGG
                     GLLWLFSDSLGVRADVRYRWGRGAAEKDNGGYGKFDNFTYLKSTLGFTVAF"
     gene            complement(2961..>4388)
                     /locus_tag="PLT35_08710"
     CDS             complement(2961..>4388)
                     /locus_tag="PLT35_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG29347.1"
                     /translation="RVAASSVRLVGTFAAGLAGLVVTGLSAGGRAVVVSAAAVVRGVT
                     AAARGAVAAAGALGRGAAAVGAAAASGAWRAGRSAASGAIRLGHACALAVRWSARALK
                     RSGVALGRAGAGAVRGVASAAGSAAQTAASMARAARPRRTVDRPGAFTWRDLPRRAGF
                     LLSDLSDRLPRARVRLSYVAAALTLIVGAVGAPYVKDRWFTPAPPSGTLRVESSRAGL
                     TVRVDGADLGPAPLTAPVPVGTHRVEVTGDGRIRVQDVAVVEGQTVVIEAGSRDLRAT
                     GSLRIDTDPPGASVWVDGVAHGTAPVTIGALAEGAHSVVVRNAAGSVRRSVAVRPDET
                     AEAVVAIRPGWLAVFAPVRLEILEGGQPIGSTEGGRLLASPGDHTIEAVSRTLGYRSS
                     HRVEVRPGEVTALTIALPPAALEIVAPPGSEIRVDGTLVGNAPLGTIQVPVGTRVVQA
                     RHRDFGDRRQSVTVTYTKAARAIFP"
BASE COUNT          601 a         1613 c         1558 g          616 t
ORIGIN      
        1 tcgtgtgcac ggcgggcgcg atgtacttgc gggcgtgggt gcccgagcgg tcgcaggtgc
       61 tgacgttcgc cgcccaccgc gtgcgccgcg tgtccgtgct cgaagagcgg ttcgtgcccg
      121 accccgaatg ggccgagcgg gccctttcgc agtcgcttgg ccccaccagc ggcccgccgg
      181 tgcacatcgt gctgcggttc gaccgcgcgc tggcgcctat ggtggccgag cgccagtacc
      241 atccaagcca ggtcgtgacg cggcatccgg acggctcgct gacactcgag ctcgacgtgt
      301 gcgacgatgt ctggctgagg agcttcgtgc tgcaattcgg ccaccgggtt cgcgtgatcg
      361 gccctccgac gctcgccgag cagatccgcg acgagctcga cgccgcgcgc cggcattacg
      421 cgccggccga ccgggtcgag gcgctggcca cgtcgcaggc tctcattgat ttgggagacc
      481 agtggcggct gcccttctga ccgcgcgcct caggatgcct tgcgctcggc cgcgctcggc
      541 agatcggcgg cgtgaaggcc ccggttttca gcgcgtcacg cctgcagccg ccgcaattcg
      601 atgtcggcca tcagtggcgc ccggtcgggc gccgcgcggc ctgcgggccg cgccgggtcc
      661 gtgacgcccg caccggcggc ggacagtctc ccggcacgca ggcggttgcg ttcggcgcgg
      721 tagcactgga aacacagcgt gtgctggcgg tccgccttga cctcgccgca gcgcgcgaac
      781 agcgcccgtc ggatccggca cgcggcgcag aagcgcgctc tcggccggac tgtcttgtcc
      841 cgccctcgcg ctcccggcgc cgcctcgtcg agcccctgcc acagccgcct cgcgtcccgg
      901 ttgccatgaa tcatcgcagc ccccctggcc tgacggcaga gagcgtacgg cccggatgtg
      961 acaggggagg tcgcgcccgc cgccccgcac gccaccgcgg gccggcggcg acggccggcg
     1021 cgcaaggcgg aaaggccggg ctggcgcatt gcgttcgccc gccaggcgga ctaggatgcc
     1081 gccgtgggag agccatgaga gcccaggttc tgcggcgcat cgcgcctgtc gacagcgacc
     1141 gcgactcgct cgagctctgc gagttgcccg acccgattcc gggccccggc gacgtcctga
     1201 ttcgggtgtc cgcctgcggg gtttgccata ccgaactcga tgagatcgaa ggccggaccc
     1261 caccgccgct gctcccggtg gttccggggc accaggtcgt cggccgtgtc gtacagtgcg
     1321 ggccgaggcc gtcggtgctc aagccgggca cgcgcgtcgg ggtcgcgtgg atttactcag
     1381 cgtgcggccg gtgcgtgtac tgcctggcgg gacaagagaa cctgtgcgca tcgttccgcg
     1441 ccaccggccg ggacgtcaac ggcggctacg cggagctgct cgtggcccgc caggacttcg
     1501 tcttcccgct gcctgacacg ccgctgaccg acgccgaggc ggcgccgctg ctgtgcgcgg
     1561 gcgccattgg ctggcggtcg ctgcgcctcg cgaacctgcc caacgggggc cggctcggct
     1621 tgaccgggtt cggcgcatcc gcgcacctgg tgctcaagct ggtgcgtcac cgcgaccctg
     1681 aggccgaagt gttcgtcttc gcccgcagcg aggcggagcg gtccttcgcg cgcgagcttg
     1741 gcgcggcgtg gacgggcgac acggcggatg cggccccgtg gcctctcgat gccatcatcg
     1801 acaccacgcc tgcgtggcgg ccggtagtcg gggcgctcaa gaacctcgcg ccgggcggcc
     1861 gcctcgtgat caatgccatc cggaaggaac acggcgaccg tgccgccctc gccgagatcg
     1921 actacccgcg cgacctctgg atggaaaagg aggtgaagag cgtcgcgaac gtgacgcggc
     1981 gggacgtgac ggagttcctg ctcgcggccg tggcgatggg ccttcgcccg gagatcgcgg
     2041 aataccccct gtccggcgcc aacgaggccc tgcggcacct caaggcgcgg gccgcccgcg
     2101 gggcgcgcgt gctgcgtctc gactagcccg ccgggagtgc gcccggccgg cggcgcccga
     2161 gccggcttcg gcttggccga ggcacgggcc ccggagtaag atgccgaggt tgccgccgcc
     2221 tgttcgggac gccgcagggt tgggcgcgac acggaagtcc cctggaggct ctatgagaaa
     2281 cgggaagctc gcccttgcgc tcctcgccgc tctagtcctg gcgccggcct ccgcgcaggc
     2341 ccagtacgcg gcccaggaag cgctcggtga ccccgatgtc tgtatcacgg ggtattacaa
     2401 cctcaacttc ggcggcgacg tctacaaagg cctgagtgcc acgacctggc acgcctcccg
     2461 ctctcatgga ctgggagcct cggtcaccct tcttgctcgt catctcgtct cggccgaggt
     2521 ggacttcaac tacagccaca gcttcttcgg cacctccgac tcgctcaaca agaactacgt
     2581 cctcacgacc acgctcgacg gcgtcgtcgg cccatggttc tcgctgggat ccgggaggat
     2641 caggacgtac gccgtggctg gcggcggcta catgcgcggc atgattacga accgccggcg
     2701 ggccggatgg tcccatccct ccaagaacct cggcgtcgtg gaggctggcg gcgggctgct
     2761 gtggctcttc agcgactcgc tgggcgtgcg cgctgacgtc cgctaccgct ggggacgagg
     2821 cgcggcggag aaagacaacg gcggctacgg caagttcgac aacttcacct acctgaagag
     2881 cacgctcggc ttcaccgtcg ccttctagac gacgcaccct gcccgaggcg cgcccgacgg
     2941 gccgtgccga cgagctcccg tcaggggaag atggcccgcg cggccttggt gtatgtcacg
     3001 gtgacgcttt ggcgccgatc gccgaaatcc cgatgccgcg cctgcacgac gcgcgtgccc
     3061 accgggacct ggatcgtgcc caggggcgcg ttgccgacga gcgtgccgtc cactcgaatc
     3121 tcggaccccg gaggagccac gatctcgagc gccgccggcg gaagcgcgat tgtcaaggca
     3181 gtaacctcgc cgggcctgac ctcgacgcga tggctcgacc ggtagcccag ggtccggctg
     3241 acggcttcga tcgtgtggtc gcccgggctg gccagcagcc gcccgccttc ggtggagccg
     3301 atgggctggc ctccttccag gatctcgagc ctgaccggcg cgaagaccgc gagccagcct
     3361 ggccggatgg ccaccacggc ctccgcggtc tcatccggcc ggacggcgac cgagcgcctg
     3421 accgaacccg ccgcgttgcg cacgacgacc gaatgcgcgc cttcagcaag agcgccgatg
     3481 gtgacgggcg ccgtgccgtg cgcgacgccg tccacccaca cgctcgcccc tggcgggtct
     3541 gtgtcgatcc ggagcgatcc ggtcgcccgc aggtccctgc tgccggcctc gatgacgacc
     3601 gtctgtcctt cgacgacggc cacgtcctgc acgcgaatgc gaccgtcgcc ggtcacttcg
     3661 acgcggtggg tgccgaccgg caccggggcg gtcaacggtg ccgggccgag gtcggcgcca
     3721 tcaacccgga ctgtgagccc cgcgcgtgag gattccaccc gcagggtgcc ggatggcggc
     3781 gccggcgtga accagcggtc cttcacgtag ggcgcgccaa cggcgccgac gatgagggtg
     3841 agggctgcgg cgacgtacga aaggcgcacg cgcgcccgcg gcagacgatc gctgaggtcg
     3901 gagagcagga atcccgcgcg tcgtggcaga tcccgccacg tgaaggcgcc gggccggtcg
     3961 acggtccgcc gcgggcgcgc ggcccgggcc atcgaggccg ccgtctgcgc cgccgacccg
     4021 gctgccgagg ccacaccacg cacggcgccc gctcctgcgc ggccaagcgc gactcccgag
     4081 cgcttcagcg cccgtgcgct ccagcgcaca gccaacgcgc acgcatgacc cagccggatc
     4141 gcgcctgacg ctgcgctgcg gcccgcgcgc cacgccccgg aagcggccgc tgcgccaacg
     4201 gcggcggcgc cacggccgag cgcgccggcg gccgccacgg cgcctctcgc cgccgctgtg
     4261 acgccgcgga cgacggcggc cgctgagacg acaacggccc ttcccccggc tgacaatccg
     4321 gtgacgacga gtccggcgag ccccgccgcg aaggtcccga caagtcggac ggaagatgcc
     4381 gccacgcg
//
LOCUS       DAOSIF010000242         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17607_length_4388_cov_3.882991, whole genome shotgun sequence.
ACCESSION   DAOSIF010000242 DAOSIF010000000
VERSION     DAOSIF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17607_length_4388_cov_3.882991"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..878)
                     /locus_tag="PLT35_08715"
     CDS             complement(156..878)
                     /locus_tag="PLT35_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG29348.1"
                     /translation="MADGFYTWFTERRLTRFGGSRRQRIERSRLALLARHAHAPGDLV
                     EIGPGTGSLQKPLTEAGWRYRAIEASPALAADLRSRGADVIEAWAPPFPLADASSDVV
                     YADQVLEHMNGIEGARAFAAESFRVLRPGGVLFVVVPDYLKERTFFWDVDYTHNFVTT
                     ERRVRQLLGDHGFRIERVVRSIGASTGIGRDLLAAASALANVPGLNALSRWTKTEDIL
                     FRVRKNFFETLAFVARKPAPPQ"
     gene            1018..2250
                     /locus_tag="PLT35_08720"
     CDS             1018..2250
                     /locus_tag="PLT35_08720"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HOG29349.1"
                     /translation="MPTLGSWLRKSVQSAAARARAGAAACGRRAGRSATFRIVLFALS
                     VRTATAALAFFSNVLFPLAQRQQFCVGERTHLLWDTFARYDSGWYFGIARYGYDFVEG
                     GRNNLAFFPVYPLLMRYLGLALGGGRENLYFAGVIISWGAYVLTMVGLYRLARTYLPR
                     AGAERTVVFASAFPFAFFYGVVYSEALFLLFTVAAFYGFRTKRWWLGALAGALASATR
                     VNGILALPALALLAWRSAGKEPAARRRALIATALVPLGLVLYSVYVDSLSGSYLEWSY
                     SIQRWNYSIAGSAVEPIVSLLRELATRPYEYLTTTPQAPYDVLNGLTALVVILSLPAV
                     GRLAGAPYVILMAANLWVPLSSGVFEGLGRYCAVLFPFFFVLAMLRPVILRKGVLVIS
                     AALYMLCLTLFTNLHPIF"
     gene            complement(2384..>4388)
                     /locus_tag="PLT35_08725"
     CDS             complement(2384..>4388)
                     /locus_tag="PLT35_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29350.1"
                     /translation="AVTPEEAARGLVPIAIAASVDGLTGARAAYDRLLSSADTAYRES
                     AAHYRRLREELTSIDSPDDRLDLAFEWGKVALDKGFVCNPHLGCGLVAGLGPSGASER
                     PGFGWFFGGDAFINAWAMTAYGDFDTVRRALEFLRQRQRADGKMMHELSQGAGYLKWF
                     EEFPYGYYHADTTPLYIIAVRDYVRASGDTALAREFWPSIERAYQYCVSTDEDGDGLM
                     DNTKAGLAAVETGALRQRDVLTDVYLGAAWTEAAEAAAELASAGGGALAPSARDAAAR
                     ARAALNRRFLDDEGRRIFFARMKDGRGQAEPTAWPGFGLWRGVFDASRPAVAGALDDL
                     AGAGVGTDWGARMLSIDSARYGPLSYNNGAVWPFLTGFAALALYAHGRPDAAWAYLDG
                     TADLTFIESRGYVAELFSGDRLRSLDAAVPHQLFATAGVVSTLLRGLLGLAALPAETR
                     DGLALPDRLAIAPQLPAGWTRLDVRNLRWRGIAADVSVRRDAAGFSAAVTPRGGTLPI
                     ELRAVLPPGSHPLARDARWRPVAATTGPSRGLSFWRVEKVSAPSVFSLRATPGIQVIP
                     VHSPLSHGDAPARLRVIDAACDGDTYTLRVEGRRGRRYDIRLLAPDVQAVTGAELLPP
                     SPADRPGERVLRVQLPPAPAAAPRAVLSRADWATIAITVRVSR"
BASE COUNT          586 a         1623 c         1563 g          616 t
ORIGIN      
        1 gcggtcggcg gccaccagcg cgagcgccag caggagccac accgggtgca cgtgcaccat
       61 cgcccgcgcc gacgcgccga gatcgatgcg ggacaggagg tacgccagga ccgcgcccgt
      121 gacgacgagg cgcgccgcga tcgagctcag cgccctcatt gcggcggggc cggtttccgg
      181 gcgacgaacg cgagcgtctc gaagaagttc ttgcggaccc ggaacaggat gtcctccgtc
      241 ttcgtccagc gggacagcgc gttcaggccg ggcacgttgg caagcgccga ggccgcggcc
      301 agcagatcgc ggccgatgcc ggtgctcgcc ccgatgctgc ggacgacccg ctcaatccgg
      361 aagccgtggt cgccgagcag ctgacggacg cggcgctcgg tcgtcacgaa gttgtgcgtg
      421 tagtccacgt cccagaagaa cgtccgttcc ttcaagtagt cggggaccac gacgaacaac
      481 accccgccgg gacgcaggac ccggaacgac tcggcggcga acgcgcgggc cccctcgatg
      541 ccgttcatgt gctcgaggac ctggtccgca tagacgacgt ccgagctggc gtcggcgagc
      601 gggaacggcg gcgcccacgc ctcgatgacg tccgcgccgc gcgaccggag gtcggccgcc
      661 agcgcggggc tggcctcgat ggcccgatag cgccacccgg cctccgtgag cggcttctgc
      721 agactgcccg tgcccggccc gatctcaacc aggtcgcccg gggcgtgcgc gtggcgggcc
      781 agcagcgcca gccggctgcg ctcgatgcgc tggcggcgcg acccgccgaa gcgggtgagc
      841 cggcgctcgg tgaaccacgt gtagaacccg tcggccatcc cgcacctccg ttgcggcgcg
      901 agtcccactc tacatcatcc ggcggtcccc atcgccgcgc caggcgtgcc gccgacccgg
      961 cttgcgctgc agcgtggcgc cccgcccggc gctggagtag aatcgcgcgc gcctgccatg
     1021 ccgaccctgg gatcgtggtt gcgcaagtcc gtccaatcgg ccgcggcccg cgcgcgcgcc
     1081 ggcgcggcgg cgtgcggccg gcgagcgggc cgcagcgcga cgttccgcat cgtgctgttc
     1141 gcgctgtcgg tccgcaccgc cacggcggcc ctcgccttct tctcgaacgt gctcttcccg
     1201 ctggcccagc gccagcagtt ctgcgtcggc gagcgcacgc acctcctgtg ggacacgttc
     1261 gcgcggtacg actccggctg gtacttcggc atcgcccgct acgggtacga cttcgtcgaa
     1321 ggaggccgca acaacctcgc gttctttccc gtctacccct tgctgatgcg gtacctgggc
     1381 ctggcgctcg gcggcgggcg ggagaacctg tacttcgccg gcgtgatcat ctcctggggc
     1441 gcctacgtcc tgacgatggt ggggctgtac cggctggcgc ggacgtacct gccgcgggcc
     1501 ggcgccgaac ggaccgtggt cttcgccagc gcgttcccgt tcgcgttctt ctacggggtg
     1561 gtgtattccg aggcgctgtt cctgctcttc accgtcgccg cgttctacgg gttccgaacg
     1621 aagcgctggt ggctgggcgc cctcgcgggc gcgctggcca gcgccacgcg ggtgaacggc
     1681 atcctggcgc tgccggcgct cgcccttctg gcctggaggt cggcgggaaa ggaacccgcc
     1741 gcgaggcgca gggccctgat cgccaccgcg ctcgtgcccc tgggactcgt gctctactcg
     1801 gtttacgtcg attcgctgag cggatcgtac ctggagtgga gctacagcat ccagcgctgg
     1861 aactactcga ttgccggctc ggccgtggag ccgatcgtgt cgctgctgcg cgaactggcg
     1921 acccggccct acgagtacct gaccaccact ccgcaggccc cctacgacgt gctcaacggc
     1981 ctgacggctc tcgtcgtcat cctgtcactg ccggccgtcg gccggctggc cggcgcgccc
     2041 tacgtgatcc tcatggcggc gaacctctgg gtgccgctgt cgtcgggcgt cttcgagggg
     2101 ctcgggcgct actgcgccgt gctgttcccg ttcttcttcg tcctcgccat gctgcggccg
     2161 gtcatcctgc ggaagggcgt gctcgtgatc tcggcggccc tctacatgct ctgcctgacg
     2221 ctcttcacca acctccatcc gatcttctga tctccggccg ccggtgcagc ggccgatatc
     2281 gagccgcggc cggacgcccg agcaggccgc cgcccgacaa gggcggcacg ttccgaggcg
     2341 cgaacgccac cgctgcgggg cacccggagc gcgccgcctg ccgtcagcgg ctgacgcgga
     2401 cggtgatcgc gatcgtggcc cagtcggcgc gggacaggac cgcccgcggc gcggcggccg
     2461 gcgccggcgg caactggacg cggagcacgc gttcccccgg gcgatcggcg ggggacggcg
     2521 gcagcagctc cgcgccggtc accgcctgca cgtccggggc gagcagccgg atatcgtagc
     2581 ggcggccgcg ccgcccctcg acgcgcaggg tgtacgtgtc gccgtcgcag gcggcgtcga
     2641 tgacgcgcag ccgggccggc gcgtcaccgt ggctgagcgg ggagtggacg gggatcacct
     2701 ggatgccagg cgtggcccga agcgagaaga ccgacggggc cgacaccttt tcgacgcgcc
     2761 agaaagacag tcccctcgag gggcctgtcg ttgctgcgac gggccgccag cgggcgtcgc
     2821 gggccagcgg gtgcgagccc ggcggcagaa cggcccgaag ctcgatgggc agggtgcctc
     2881 cccgcggggt gacggccgcc gagaaaccgg ccgcatccct cctgacggag acatcggcgg
     2941 caatcccgcg ccatcgcagg ttgcggacgt cgagccgcgt ccagcccgcc ggaagctggg
     3001 gcgcgatcgc caggcggtcg gggagcgcga ggccgtcgcg ggtctcagcc ggcagcgcgg
     3061 ccaggccgag cagcccccgc agaagcgtcg agacgacgcc ggccgtggcg aagagctgat
     3121 gaggcacggc cgcgtcgagg gaccgcaggc ggtcgccgga gaacagctcc gcgacgtacc
     3181 cgcgcgactc gatgaaggtc aggtccgccg tgccgtccag gtaggcccac gccgcatcag
     3241 gcctgccgtg cgcgtacagc gccagcgcgg cgaagcccgt caggaacggc cacaccgcgc
     3301 cgttgttgta gctgagcggg ccgtagcgcg cgctgtcgat cgacagcatg cgcgcgcccc
     3361 agtccgttcc caccccggcg cccgcgagat cgtcgagcgc gccggccacg gccggccggg
     3421 acgcgtcgaa caccccccgc cagaggccga atcccggcca cgcggtcggc tcggcctgtc
     3481 cccgcccgtc cttcatccgc gcgaagaaga tccgtcgccc ctcatcgtcc aggaagcgcc
     3541 ggttcagcgc ggcccgggcc ctggccgcgg cgtcgcgggc cgagggggcc agcgcgccgc
     3601 ccccggcaga ggccagttcg gccgccgcct ccgcggcctc ggtccatgcc gcgccgaggt
     3661 agacgtcggt cagcacgtcg cgctggcgga gcgcgccggt ttcgacggcg gccaggccgg
     3721 ccttcgtgtt gtccatcaat ccgtcgccat cctcgtcggt ggagacgcag tactggtagg
     3781 cccgctcgat tgacggccag aactcgcggg cgagggccgt gtcgccgctt gcgcggacgt
     3841 agtcgcgcac cgcgatgata tagagcggcg tcgtgtcggc gtggtagtag ccgtacggga
     3901 actcctcgaa ccacttcagg taccccgcgc cctgcgacag ctcgtgcatc atcttgccgt
     3961 cggcccgctg gcgctggcgc aggaactcaa gcgcccgcct caccgtgtcg aagtcgccgt
     4021 acgcggtcat ggcccacgcg ttgatgaacg cgtcgccccc gaagaaccag ccgaacccgg
     4081 ggcgctcgct cgcgcccgac ggccccaggc cggcgacgag accgcagccc aggtgcggat
     4141 tgcagacgaa gcccttgtcg agggcgacct tgccccactc gaacgcgagg tcgagccggt
     4201 cgtcggggct gtcgatgctc gtcagctcct cgcgcagcct gcggtagtgc gccgcggatt
     4261 cccggtacgc cgtgtcggcc gacgagagca ggcgatcgta tgccgcccgc gcgcccgtca
     4321 acccgtccac gctcgcggcg atcgcgatcg gcacgaggcc ccgcgccgcc tcctctggcg
     4381 tcacggcg
//
LOCUS       DAOSIF010000243         4382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17644_length_4382_cov_3.629304, whole genome shotgun sequence.
ACCESSION   DAOSIF010000243 DAOSIF010000000
VERSION     DAOSIF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17644_length_4382_cov_3.629304"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..2190
                     /locus_tag="PLT35_08730"
     CDS             304..2190
                     /locus_tag="PLT35_08730"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOG29351.1"
                     /translation="MRRPNIAGVAALAAAVLAAVANRPADGPPVDYPGAPVPAAAVTF
                     NDGFWSSRLATVEAVTLGAAFRQSELTGRIRNFEIAGGEADGAFCTRYSFDDSDVFKL
                     IEGASYALARRANPALDAYLDALIAKIAKAQESDGYLFTARTIDPARTMPMAGSARWT
                     NERDSHELYNAGHLYEAAVAHYQATGKKTLLDVATKNADLVDRTFGPAGLRYPPGHQE
                     IEIGLVRLFRVTGNRRYLTLAKFFLDERGNAAGHQLYGEYAQDHKPVVDQDEAVGHAV
                     RAAYMYTAMADVAALTGAARYVAALDRIWDDVVLRKLYLTGGIGAEGEWEGFGPAYEL
                     TNSAYAETCASIANALWNHRMFLLKLDARYMDVYERAVYNAMLSGLSLAGDTFFYPNP
                     LLSLGQHARSPWFACACCPSNLPRFILSVPSHVYATAGDRVFVSLFAQGRARVSTPVG
                     TVGIEQQTRYPWDGDVRVTVSPARPARFTLMVRVPGWSLGRPVPGDLYRDAEPVRDSV
                     SLKVNGLLVPIRLERGFAAIAREWAAGDTVDLHLPMPVRRVVAHPAVAADEGRVAIER
                     GPIVYAAESVDHGGRVTNLVVDSAPSWQPSGALRNWAASSPLRAGRRRSACRKAPSSR
                     SACR"
     gene            2151..2711
                     /locus_tag="PLT35_08735"
     CDS             2151..2711
                     /locus_tag="PLT35_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29352.1"
                     /translation="MQEGAIQSQRVPLTLIPYYAWAHRGPGEMAVWLARDPAKARPAP
                     EPTVASQARVSASEGARGLEGLNEQYEPARSGDREPLYMHWRPKTGSLEWVQYDLPPD
                     TTVSEASVYWYADTSEGGCRAPRTWRLVYKRGDAWLPVETSGEYGVALDRYNTVRFTP
                     VTTTAVRLEVSLPEGFSAGIHEWKLR"
     gene            2720..3466
                     /locus_tag="PLT35_08740"
     CDS             2720..3466
                     /locus_tag="PLT35_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOG29353.1"
                     /translation="MPHVLLEICCGSLDDALEAGAGGADRVELCSSLFHGGLTPSFGT
                     LVEAKARLDIPVIFMARPRGGGFCYTATEMAAMERDTEMAVAQGADGVVFGILTEDGR
                     IDLPRTRRLRDRVGDRDAVFHRAFDVTPDPFRALDELIDLGITRVLTSGQCDTVWEGM
                     PLIARLIDYAAGRIQIMPGGGIKPFHVDEVIARTGCRQIHIAAWKTERDDSTRHRPAV
                     TFGGALYPPENLYDVTDRAVVRDIAHRLKR"
     gene            3533..3904
                     /locus_tag="PLT35_08745"
     CDS             3533..3904
                     /locus_tag="PLT35_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG29354.1"
                     /translation="MAEPKAKVCHYTDVPAEPVGLRAPGATMRWLIDDARDGAPVYAL
                     RMVEIGPGGNSPHHQHNYEHENFVVEGKGRVFLAGEWHDLKPGDVVFVPPGVEHEYVN
                     AGDTTFRFLCGIPVTKLRPSP"
     gene            3918..>4382
                     /locus_tag="PLT35_08750"
     CDS             3918..>4382
                     /locus_tag="PLT35_08750"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG29355.1"
                     /translation="MRRRPCFGAVVLALALVLWPARARPSQAAQQPPITEPAPGAIDR
                     IFAPVDRTSSPGCAVGVASRGRLAHLQGYGMANLEYGVPIRPLTVFEAGSVAKQVTAA
                     AIVLLAQDGKLSLDDPVRTHVPELPEFGAPILVRHLLGHTSGLRSQWPMLTMA"
BASE COUNT          669 a         1519 c         1563 g          631 t
ORIGIN      
        1 agagcagctt ggccaggatc ggcagggaga ccagcagcag cgtgatcgcc atgaagtagt
       61 cgtcccacga cgggcgatcg gccgacgcgt ccaggacccg ggcgatgtgc tcgacgagcg
      121 acggcgtggt gtctgccaag ggcacctccc cggccgggcc ggcttgccga catcgtagca
      181 gactggcggg cggcccgggg cgaagcgctc gcgttgatcc ccggacttgc gctagattca
      241 gcctgcggcg gcgcgcgggc gccgatggcg cggccgtccg atgggcccct ggggaactcg
      301 agcatgcgga gaccgaacat cgccggcgtc gccgccctcg cggccgccgt cctggccgcg
      361 gtcgcgaacc ggccggcaga cggcccgccc gtggactatc cgggcgcacc cgtcccggcc
      421 gccgcggtca cgttcaacga cggcttctgg tcctcccgcc tcgcgacggt cgaggcggtg
      481 acgctcggcg cagccttcag gcagagcgaa ctgaccgggc ggatcaggaa cttcgagatc
      541 gccggaggcg aggcggacgg cgcgttctgc acccgctact ccttcgacga ctccgacgtg
      601 ttcaagctca ttgaaggcgc gtcgtacgca ctggccaggc gcgccaaccc ggcgctggat
      661 gcctacctcg acgcgctgat cgcgaagatc gccaaagcgc aggagtccga cggctacctg
      721 ttcaccgccc ggacgatcga tcccgcccgg accatgccga tggccgggag cgcgcgttgg
      781 acgaacgagc gggacagcca cgagctgtac aacgcgggac atctctacga ggctgccgtc
      841 gcgcactacc aggccaccgg gaagaagacc ctgctggacg tggccacgaa gaacgccgat
      901 ctcgtggatc ggacgttcgg gcccgccggc ctccggtatc cgccggggca ccaggagatc
      961 gagatcgggc tggtcaggct cttccgcgtc acgggcaacc ggcggtacct gacgctcgcc
     1021 aagttcttcc tggacgagcg cgggaatgcc gcggggcacc aactgtacgg cgagtacgcg
     1081 caggatcaca agccggtcgt ggaccaggac gaggccgtcg gccacgcggt ccgcgccgcg
     1141 tacatgtaca cggccatggc ggacgtcgcc gccctgacgg gcgcagcccg ttacgtggcc
     1201 gccctcgacc gcatctggga cgatgtcgtg ctccggaagc tctacctgac cggcggcatc
     1261 ggcgccgagg gcgagtggga gggattcggc ccggcctatg agctcacgaa ctcggcgtac
     1321 gcggaaacct gcgcctccat cgccaatgcg ctctggaacc accggatgtt cctgctgaag
     1381 ctggacgcgc gctacatgga cgtctacgag cgcgccgtgt acaacgcgat gctctcgggc
     1441 ctctcattgg cgggggacac gttcttctac ccgaaccctc tcctgtcgct gggacagcat
     1501 gcgcgcagcc catggttcgc gtgcgcctgc tgcccgagca acctgccccg cttcatcttg
     1561 tcggtgccct cgcacgtgta cgcgacggcg ggcgaccggg tgttcgtcag cctgttcgcg
     1621 cagggccggg cgcgggtctc gacgccggtc gggaccgtcg ggatagaaca gcagacacgg
     1681 tatccttggg acggcgatgt ccgtgtcacg gtctcgccgg cgagaccggc ccgtttcacg
     1741 ctgatggtga gggtgcccgg ctggtcgctc ggcaggccgg tcccgggcga cctctaccgg
     1801 gacgcggagc ctgtccgaga ctcggtgtcg ctcaaggtca acggcctgct cgtgccgatc
     1861 aggctcgagc gcggcttcgc cgccatcgcc cgggaatggg cggccggcga cacggtggac
     1921 ctgcaccttc cgatgccggt ccgccgcgtg gtggcgcacc ccgcggtcgc cgccgacgag
     1981 ggccgcgtgg ccatcgagcg gggcccgatc gtctacgccg ccgagtccgt ggatcacggc
     2041 ggacgcgtca cgaacctggt ggtcgacagc gcgccgtcgt ggcaaccgag tggcgccctt
     2101 cgcaactggg cggcatcgtc gccattacgg gccgggcgac ggcgctcagc atgcaggaag
     2161 gcgccatcca gtcgcagcgc gtgccgctga cgctcattcc gtactacgcg tgggcgcacc
     2221 gagggccagg ggagatggcg gtctggctcg cgcgcgatcc cgccaaggcc aggccggcgc
     2281 cggagcccac ggtggcgtcg caggcgcgcg tctcggcgtc cgaaggcgcc cgcgggctgg
     2341 aggggctcaa cgaacagtac gagccggcgc ggtcgggcga ccgggaaccg ctgtacatgc
     2401 actggcgccc caagacgggc agcctcgaat gggtccagta cgacctcccg cccgacacga
     2461 ccgtctccga ggcgtccgtc tattggtatg ccgacacgag cgagggcggc tgccgggcgc
     2521 cgaggacctg gcgcctcgtg tacaagcggg gcgacgcatg gctgccggtg gaaaccagcg
     2581 gcgagtacgg cgtggcgctc gaccgttaca ataccgtccg cttcacgccg gtgacgacga
     2641 ctgccgtccg gctggaggta agcctgcccg agggattctc cgcgggcatt cacgagtgga
     2701 aactcaggta ggaggccgga tgccgcacgt gctgctggag atttgctgcg ggtccctcga
     2761 cgacgcgctc gaggcgggtg cgggcggagc ggaccgcgtc gaactgtgct cctcgctctt
     2821 ccatggaggc ctgactccgt cgttcggcac cttggtcgaa gccaaggccc gtctcgacat
     2881 ccccgtgatc ttcatggccc gcccgcgtgg cggggggttc tgctacaccg ccacggagat
     2941 ggcggccatg gagcgcgaca cggagatggc cgtggcccaa ggcgccgacg gcgtggtgtt
     3001 cggcatcctc accgaggacg gccgaatcga cctgcctcgg accaggcgcc ttcgcgatcg
     3061 cgtcggcgac cgggacgcgg tgttccaccg cgcgttcgac gtcacgcccg atccgttccg
     3121 ggcgctcgac gagctgatcg atctgggcat cacgcgcgtc ctgacgagcg ggcagtgcga
     3181 cacggtctgg gaggggatgc cgttgatcgc gcggctcatc gactatgccg ctggccggat
     3241 ccagatcatg ccaggcggcg gcatcaagcc gttccacgtg gatgaggtga tcgcgcggac
     3301 gggctgccgc cagattcaca tcgcggcgtg gaagaccgag cgcgacgact ccacgcggca
     3361 ccgtcccgcc gtcaccttcg gcggggcgct ctatccgccg gagaacctgt acgacgtgac
     3421 ggatcgggcc gtcgtgcgcg acatcgcgca ccggctcaag cgctgagagc aggcgacccc
     3481 gaaggccagg gcgaagcccc ggccggggtg tcgcggaacc agggagcaga ccatggccga
     3541 gccgaaagcc aaagtgtgtc actacaccga cgtgccggca gaacccgtcg gcctgcgcgc
     3601 gccaggggcg acgatgcggt ggctgatcga cgatgcccgc gacggcgcgc ccgtctacgc
     3661 cttgcggatg gtggagatcg gcccgggcgg caactcgccg caccaccagc acaactacga
     3721 acacgagaac ttcgtcgtcg agggcaaggg gcgcgtgttc ctcgccggcg agtggcacga
     3781 cttgaagccc ggggacgtgg tgttcgtgcc gcccggcgtc gagcacgagt acgtgaacgc
     3841 gggcgacacg acgttccggt tcctctgcgg gatccccgtc acgaagctgc ggccgtcgcc
     3901 gtgaaaggag acgagtcatg cgccggcgtc catgcttcgg ggcggtcgtg ctggccctcg
     3961 ctctcgtcct ctggccggcg cgcgcgcgcc cgtcccaggc agcccagcag ccccccatca
     4021 cggagcccgc gccaggggcc atcgacagga tcttcgcccc tgtcgaccgg acgtcgtcgc
     4081 cgggctgcgc cgtcggcgtg gcctcgcggg gccgactggc ccacctgcag ggatacggca
     4141 tggccaacct ggagtacggc gtgccgatcc ggcccctgac cgtgttcgag gcagggtcgg
     4201 tcgcgaagca ggtcacggcc gctgccatcg tgctcctcgc ccaggacggc aagttgtcgc
     4261 tcgacgatcc ggtgcggacg cacgtgccgg agctgccgga gttcggcgcc ccgatcctcg
     4321 tcaggcacct gctcggccac acgagcggcc ttcggagcca atggccgatg ctgacgatgg
     4381 cg
//
LOCUS       DAOSIF010000244         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17697_length_4374_cov_3.667979, whole genome shotgun sequence.
ACCESSION   DAOSIF010000244 DAOSIF010000000
VERSION     DAOSIF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17697_length_4374_cov_3.667979"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="hydG"
                     /locus_tag="PLT35_08755"
     CDS             <1..893
                     /gene="hydG"
                     /locus_tag="PLT35_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOG29356.1"
                     /translation="ANVAPLTLDEFRQLKAANIGTYQLFQETYHRPTYAAVHIAGKKR
                     DFDWRLTAMDRAMQAGIDDVGIGVLFGLFDWRFELLALMQHIRHLESRFGVGPHTISV
                     PRMEPASGSDVASQPPHPVSDVDFRKIVAILRLAVPYTGIIMSTRESAHIRRETFALG
                     VSQISAGSRTNPGGYADDEHFEAAQFSLGDHRPLDEVIRDVAGLGYIPSFCTGCYRLG
                     RTGQDFMDLAKPGDIKAHCDPNALSTFVEYLIDYASEETRKVGDALVEEALASMEPGP
                     RRAATTMVAKVRGGKRDVFC"
     gene            994..1650
                     /locus_tag="PLT35_08760"
     CDS             994..1650
                     /locus_tag="PLT35_08760"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOG29357.1"
                     /translation="MVHGHLKPVSSRLTVQTIGRLSLYRRLLLEVSASGARQIYSHQL
                     ATAAVATPAQVRRDLMAIGFSGSPRRGYAVDDLIAAINAVLARNVETSVALVGVGNLG
                     RAILAYFANREPWVRFTAAFDRDPDKVNRILHGCRVYPIDRAEEIIRRDGIRAAAIAV
                     PASEAQHTADRLVLAGVRSFLNFAPVRLHVPTGTFVDDVDMTTALDRVAFYAHQPQGS
                     "
     gene            1672..2145
                     /gene="nuoE"
                     /locus_tag="PLT35_08765"
     CDS             1672..2145
                     /gene="nuoE"
                     /locus_tag="PLT35_08765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG29358.1"
                     /translation="MTAPDFDPIVAKYPSGNRDALIPLLQDVQQQCGYLPREAVVRIG
                     QHLQLPASKVYGVATFFNQFRFQPLGRYHFQVCRGTACHVKKSMQTLTALQRTLNIKP
                     GQTSRDGLFSLEVVACLGACGLAPVVAVNGEFHAGASPDLVNRLVRTLRKEAKVK"
     gene            2142..4136
                     /locus_tag="PLT35_08770"
     CDS             2142..4136
                     /locus_tag="PLT35_08770"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOG29359.1"
                     /translation="MTHASFPRCCAECWHSPERPCPDLVACLASGPLCHESEACASWR
                     TRRQVELRREAPVRPVIFVGTGTCGLGAGAGRTLAAVRAWVDAGAREADVVEVGCIGL
                     CVEEPLVDVQMPGRPRLSFARVSEKKVGPLLDAIFDGHPLPVEPLGQFRHAGATRWPG
                     VAALDAHPFFALQTRWVLEHCGILDPASLDEYLARGGYTALAKALRTMTPAEVCAQVE
                     QSGLRGRGGGGFPTGTKWSIANKQAADQKYMVCNADEGDPGAFMDRAIMESDPHRVLE
                     GLALAAYAIGASKAYIYTRAEYPLAIQRMNAAIADATAYGLLGANVFDSGFSLDIHVK
                     QGAGAFVCGEETALLASIEGRRGMPRPRPPYPAIAGLFGKPTVINNVETLANVPPIVA
                     KGAAWFASIGTASSKGTKVFALSGNVARTGLVEVAMGTALRRIVFEIGGGIPGDRAYK
                     AVQIGGPSGGCIPTSELDIDIDYESLKSVGAMMGSGGLVVMDDGSCMVDVAKFFMDFI
                     QKESCGKCIPCREGTRRMLEILESLTRSRRSEERFEALERFRAVMNLEQLAGVIKDTS
                     LCGLGQSAPNPVLSTLRWFRGEYEAHVFDRRCPAGACRELLVYEIDPVRCKGCGLCVK
                     QCPSDAIVGALKSPHHIIPERCIACGTCVDVCNLDAVLVH"
     gene            4225..>4374
                     /locus_tag="PLT35_08775"
     CDS             4225..>4374
                     /locus_tag="PLT35_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG29360.1"
                     /translation="MTLEINGRSVDVHAGDTVLAAARRAGIRIPTLCHYEGLPPSGAC
                     RMCVVE"
BASE COUNT          662 a         1530 c         1523 g          659 t
ORIGIN      
        1 acgccaacgt ggcgccgctc acgctcgacg agttccgtca gctcaaggcg gcgaacatcg
       61 gcacctacca gctcttccag gagacctacc accgacccac ctacgccgcc gtccacatcg
      121 ccggaaagaa gagggacttc gattggcgcc tgacggcgat ggaccgcgcc atgcaggccg
      181 gcatcgacga cgtgggcatc ggggtgctgt tcgggctgtt tgactggcgc ttcgagctgc
      241 tggcgctcat gcagcacatc cggcacctcg agtcgcgctt cggcgtcggt ccgcacacga
      301 tcagcgtgcc ccggatggag ccggcgagcg ggtccgacgt ggccagccag ccgccgcacc
      361 cggtctcgga cgtcgacttc cggaagatcg tcgccatcct caggctggcg gtgccctaca
      421 ccggcatcat catgtccacg cgtgagtcgg cgcacatccg gcgcgagacc ttcgccctcg
      481 gcgtgtcgca gatttcggcg ggcagccgga cgaacccggg cggatacgcc gacgacgagc
      541 atttcgaggc ggcgcagttt tcgctcggcg accaccggcc gctcgacgag gtcatccgcg
      601 acgtcgcggg cctcggctat attccgtcct tctgcacggg gtgctaccgt ctgggccgga
      661 cgggccagga cttcatggat ctggccaagc cgggcgacat caaggcgcac tgcgacccaa
      721 acgcgctgtc cacctttgtc gagtacctca tcgactacgc gtcggaggag acgcgaaagg
      781 tcggggacgc gctggtcgag gaggcgctgg cctcgatgga acccgggccg aggcgagccg
      841 cgacgacgat ggtggccaag gtgcgcggcg gcaagcggga cgtgttctgc tgaggcgttt
      901 cctgaccgcc cggagttggc tcttgacacg gttcgtttga gtgcgtaagc tatctgtgac
      961 tgtattcaca gcgtgaaaca cgtcacagat aacatggttc acggacacct caaaccagtc
     1021 tcatcccggc tcaccgttca aacgatcggc cgcttgagcc tgtaccgccg cctcctcctt
     1081 gaggtttccg cgtcgggcgc gcgccagatc tactcccatc aactcgccac cgcggccgtg
     1141 gccacgccgg cgcaggtccg gcgggacctc atggccatcg gcttttccgg cagcccccgc
     1201 cggggctacg cggtcgacga cctcatcgcg gccatcaacg ccgtcctcgc ccggaacgtc
     1261 gagacctccg tggccctggt cggcgtcggc aatctcgggc gcgcgatcct ggcctatttc
     1321 gcgaaccgcg agccgtgggt ccgcttcacg gcggccttcg accgcgaccc ggacaaggtg
     1381 aaccggatcc tccatgggtg ccgcgtctac cccatcgacc gcgccgagga gatcatccgc
     1441 cgcgacggca tccgggccgc tgccatcgcg gtgccggcct ccgaggccca gcacacggcg
     1501 gaccgcctgg ttcttgctgg cgtccgcagc ttcctcaact tcgcgccggt acgcctgcac
     1561 gtgccgaccg gcacgttcgt cgacgacgtt gatatgacga cggccctcga ccgtgtggcg
     1621 ttctacgcgc accagccgca gggctcgtga gccccccggg atgcatgcct catgacagcg
     1681 cctgacttcg acccgatcgt tgccaagtac ccctccggga accgggacgc cttgatcccg
     1741 ctgctgcagg atgtccagca gcagtgcggc tacctgcccc gggaggcggt ggtccgcatc
     1801 ggccagcacc tgcagctgcc cgccagcaag gtgtacggcg tggccacctt tttcaaccag
     1861 ttccggttcc agccgctggg ccggtatcac ttccaggtct gccgaggcac ggcgtgccac
     1921 gtgaagaagt cgatgcagac gctgaccgcg ctgcagcgca cgctgaacat caagcccggc
     1981 cagacgtctc gcgacgggct gttcagcctg gaggtggtgg cctgcctcgg ggcgtgcggt
     2041 ctcgccccgg tggtggccgt gaacggcgag ttccacgccg gcgcgtcacc cgacctcgtc
     2101 aaccgcctgg tgcggacgct ccggaaggag gcgaaggtca agtgacgcac gcgtcattcc
     2161 cccgctgctg cgccgagtgc tggcactccc ccgaacggcc ctgcccggac ctggtggcct
     2221 gcctggcgag cggtccgctc tgccacgaaa gcgaggcctg cgcctcctgg cgcacgcggc
     2281 gccaggtcga gctgcgccgg gaggcccccg tccgcccggt gatcttcgtc ggcaccggga
     2341 cctgcggcct gggcgctggc gccggacgga cgctggccgc cgtccgcgcc tgggtcgacg
     2401 ccggggcgcg cgaggcggac gtggtcgagg tcggctgcat cggcctctgc gtcgaggagc
     2461 cgctggtgga cgtgcagatg cccggccgtc cccggctgag cttcgcccgc gtgagcgaga
     2521 aaaaggtggg cccgctcctg gacgcgatct tcgacgggca cccgctgccg gtcgagccgc
     2581 tcggccagtt ccggcacgcc ggcgccactc gctggcccgg cgtggcggcg ctcgacgccc
     2641 atcccttctt cgccctgcag acgcgctggg tgctggagca ctgcggcatt ctcgatccgg
     2701 cgagcctgga cgagtacctc gcccgcgggg gctacaccgc cctcgccaag gcgctgcgga
     2761 cgatgacgcc ggcggaggtc tgcgcccagg tcgagcagag cggcctgcgg ggacgcgggg
     2821 gcggcggctt cccgacgggc accaagtggt cgatcgcgaa caagcaggcg gcggatcaga
     2881 agtacatggt ctgcaacgcc gacgagggcg acccgggggc gttcatggat cgcgcgatca
     2941 tggagagcga cccgcaccgc gtcctcgaag gcctggcgct cgccgcgtac gccatcggcg
     3001 cgagcaaggc ctacatctac acgcgcgccg agtatccgct ggccatccag cggatgaacg
     3061 ccgccatcgc cgacgccacg gcgtacggcc tgctgggcgc gaacgtcttc gactcgggct
     3121 tctcgctcga catccacgtc aagcagggag ccggggcctt cgtgtgcggc gaggagacgg
     3181 cgctgcttgc aagcatcgag gggcgccgcg gcatgccgag gccgaggccg ccctatccgg
     3241 ccatcgccgg gctcttcggc aagccgacgg tcatcaacaa cgtcgagacg ctcgccaacg
     3301 tcccgcccat cgtcgcgaag ggcgcggcgt ggttcgcgtc catcggcacc gccagcagca
     3361 agggcaccaa ggtgttcgcg ctgtcgggca acgtcgcccg gacgggcctc gtcgaggtcg
     3421 cgatgggcac ggcgctccgg cggatcgtgt tcgagatcgg cggcggcatc cccggcgacc
     3481 gcgcctacaa ggcggtccag atcggcgggc cgtccggcgg atgcatcccg acgtcggagc
     3541 tcgacatcga catcgactac gagtcgctga agtcggtggg cgcgatgatg ggctccggcg
     3601 gcctggtcgt catggacgac gggagctgca tggtggacgt cgccaagttc ttcatggact
     3661 tcatccagaa ggagagctgc ggcaagtgca tcccgtgccg cgagggaacg cggcggatgc
     3721 tcgagatcct ggagagcctg acgcggagcc gccggtcgga ggagcgcttc gaggccctcg
     3781 agcggttccg ggcggtgatg aacctcgagc agctggccgg cgtgatcaag gacacgagcc
     3841 tctgcggcct gggccagtcg gcgccgaacc cggtgctgag cacgctccgc tggttccgcg
     3901 gggagtacga ggcgcacgtc ttcgaccgcc gctgccccgc cggcgcgtgc cgcgagctgc
     3961 tcgtctacga gatcgacccg gtgcgctgca agggctgcgg cctctgcgtc aagcagtgcc
     4021 cgtccgacgc catcgtgggc gcgctgaaga gcccgcacca catcatcccc gagcgctgca
     4081 tcgcctgcgg cacgtgcgtc gacgtatgca acctggacgc ggtgctggtg cactagctcg
     4141 gcaggggccg gagcccgggg gcgaggcgcg agcagcgagg ggccgggcag ggaacaaggg
     4201 ctgaggggca aggacaaggc gatgatgaca ctcgagatca atggccggtc ggtcgacgtg
     4261 cacgccggag acacggtgct cgcggcggcc cggcgggccg gcatccggat tccgacgctc
     4321 tgccattacg agggcctgcc gccgagcggc gcgtgccgca tgtgcgtggt cgag
//
LOCUS       DAOSIF010000245         4367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17736_length_4367_cov_3.927412, whole genome shotgun sequence.
ACCESSION   DAOSIF010000245 DAOSIF010000000
VERSION     DAOSIF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17736_length_4367_cov_3.927412"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLT35_08780"
     CDS             complement(<1..615)
                     /locus_tag="PLT35_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOG29361.1"
                     /translation="MKIGVFAVLFGQKPFEETLDYLVDLGIGAVEIGTGCYPGNAHAN
                     PAVLLASDRRLKAFKEAIERRNLVISALSCHGNPLHPHAATAKAHHRVFEQTVELASK
                     LGVENVITFSGCPGADAKAVTPSWVVSPWPPEFAQALAWQWAERVVPYWKQTAAICRK
                     AGVRVAIEMHPNFVVYNAETMNRLRAIAPAVIGCNFDPSHLFWQG"
     gene            complement(612..1745)
                     /locus_tag="PLT35_08785"
     CDS             complement(612..1745)
                     /locus_tag="PLT35_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29362.1"
                     /translation="MSEINVALIGTAFMGRAHGNAWRQVAAFCRPKRTPRLKVICGRD
                     PGRTKAAARAFGWEEAATDWREVVRRPDIGIVDVCTPGDSHAEIAIAAARAGKAVLCE
                     KPLANTVREAERMLAAAERAGVAHMVCHNFRRIPAVCLARQIIDEGRLGEIRHFRGAY
                     LEDWLLNPAFPLVWRLDRKRAGSGSLGDIGSHVIDLARMLAGEIREVSGLTETFIRTR
                     PLPGSPARKGRVTVDDAAVALVRFASGAVGSIEATRFAPGRKSQCSFEINGSRGSLAF
                     DLERLNELQVYFESDPPALRGFRTILATEPIHPHMAPWWPPGHIIGWEHSFTHMVLDL
                     LAAVADGTVPSPSFHDGVMNQRVLDAIGRSSASRRWARVKGVA"
     gene            complement(1796..3808)
                     /locus_tag="PLT35_08790"
     CDS             complement(1796..3808)
                     /locus_tag="PLT35_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29363.1"
                     /translation="MFPAPLRIFLASFLVLFLEVALIRWLPAQIRLLSYFSNFILLAA
                     FLGIGIGSLLGRAARSLFAWYPALQLLLVAVVYFFRLEVRINAPGSIYFTSGTSDPVT
                     AVETTLLLPVIFVAVAALFAALAQRMAAEMTALPPLRAYTLNLAGSLAGTAAFALMSW
                     LEAPPSAWFIASLAAAVPFLRARRWTTASLAAVLCLVTGAGLVYDMQRGSLWSPYYKI
                     TLKRAGPETVVEVNNIWHQSMARVNAKEYFYQWPYMAFGDTFHDALILGAGTGTDVAA
                     ALAHGVASVDAVEIDPAILRLGRQGHPDRPYADPRVHAVADDARHYLRTTTKKYDLVV
                     FALIDSLTLQSAFSGVRLESYMFTEESFRAVRDVLAPDGVLVVYNYFREPWLVDRLAN
                     TAAAAFGMEPMVHVHPAKDQLGVIVVGPRLRTLARYPDPPSEVTAFNQGSPLAPPVRH
                     ERDRAVAPATDDWPFLYMRRPHLPSHYTSALAVVLLVSAVAVFGVVRGAARKWSWEFF
                     FLGAGFMLLESKSIIQFALLWGSTWVVASLIISSVLVMALLANWVVARVEIRRPWLVG
                     AVLGALLALNYLVPVGAVGFESRALESLVYSVMVFSPIFCAGLLFGSSIKRSGDVTID
                     YGANLLGAMVGGVAEYLSLVTGFQFLLILAAAFYLAALATRKPMTA"
     gene            complement(3873..>4367)
                     /locus_tag="PLT35_08795"
     CDS             complement(3873..>4367)
                     /locus_tag="PLT35_08795"
                     /inference="COORDINATES: protein motif:HMM:NF023454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOG29364.1"
                     /translation="EWERMRDPDYQPHGAGARPGPDVKPPGPAAAREDVTRDRKAFLA
                     AVRARLFTFLRAWWVGDYAGALAALDGTGDGGGEWTADRLEAARAGYRAEHGPLRFDP
                     EARNLRHTHVSDAPEGEALRVEQMLVDPDMHNDWVAEFRVDVAASREAGRPVLALVRL
                     GSLA"
BASE COUNT          689 a         1535 c         1580 g          563 t
ORIGIN      
        1 cccctgccag aacaggtgcg acgggtcgaa gttgcatccg atgaccgccg gggcgatcgc
       61 gcgcaggcgg ttcatggtct cggcgttgta gacgacgaag ttcgggtgca tttcgatcgc
      121 cacccgcacg ccggccttgc ggcagatcgc cgccgtctgc ttccagtacg gcaccacgcg
      181 ctcggcccac tgccacgcga gggcctgggc gaactccggg ggccacggcg acacgaccca
      241 gctcggcgtg acggcctttg catccgcgcc cgggcacccg ctgaaggtga tcacgttctc
      301 aacgccaagc ttcgaggcca gctcgacggt ctgctcgaag accctgtgat gggccttcgc
      361 cgtcgcggcg tgcgggtgga ggggattccc gtggcagctc agcgcgctga tgacgaggtt
      421 gcgccgctcg atggcttcct tgaacgcctt cagcctgcgg tcgctggcca gcagcacggc
      481 cgggttggcg tgggcgttcc ccgggtagca gcccgtgccg atttcaacgg cgccgatccc
      541 caggtcgacg aggtagtcca gggtttcctc gaacggcttc tggccgaaca ggacggcgaa
      601 cactccgatc ttcatgcgac cccctttacg cgcgcccagc ggcgcgacgc ggacgagcgc
      661 ccgatggcgt cgagcacccg ctggttcatg acgccgtcgt ggaagctggg cgacggcacc
      721 gtgccgtcgg ccacggcggc gagcagatcg agcaccatgt gcgtgaacga gtgctcccat
      781 ccgatgatat ggcccggcgg ccaccacggc gccatgtgcg ggtggatcgg ctcggtcgcc
      841 aggatcgtcc ggaacccgcg cagagccggc gggtccgact cgaagtagac ctggagctcg
      901 ttgagccgct cgaggtcgaa ggccaggctt ccccggctcc cgttgatctc gaacgagcac
      961 tggctcttcc ggcccggggc gaaccgcgtg gcctcaatgg atccgacggc gccgcttgcg
     1021 aaccgcacga gcgccaccgc cgcgtcgtcc accgtcacgc gccccttgcg ggcgggcgaa
     1081 ccgggcagcg ggcgcgtccg aatgaacgtc tcggtcaggc cggacacctc gcggatctcg
     1141 cccgccagca tgcgcgccag atcgatcacg tgcgacccga tgtcgccgag cgatccggag
     1201 cccgcccgct tccgatcgag gcgccacacg agcgggaacg cggggttcag cagccagtcc
     1261 tcgaggtagg cgccccggaa gtggcggatc tcgcccaggc gcccctcgtc gatgatctgc
     1321 ctggcgaggc agacggccgg gatccggcgg aagttgtggc acaccatgtg ggccacgccg
     1381 gcgcgctcgg cggcggcgag catccgctcg gcctcccgca cggtgttcgc caggggcttc
     1441 tcgcagagga cggccttgcc ggcccttgcc gcggcgatgg cgatctcggc atggctgtcc
     1501 ccgggcgtgc agacgtcgac gatgccgatg tccggacgac ggacgacctc ccgccagtcg
     1561 gtggccgcct cttcccagcc gaacgcgcgc gcggcggcct tcgtccgccc ggggtcccgg
     1621 ccgcagatga ccttcaggcg cggcgtccgc ttcgggcggc agaacgccgc cacctggcgc
     1681 caggcgttgc cgtgggcgcg gcccatgaac gcggtgccga tcagggcgac gttgatctcg
     1741 gacatgcgca cgattctaca ccggggcgcg ccggcatcgg gcgccggccc ggcggctacg
     1801 ccgtcatcgg cttgcgcgtg gcgagggcgg ccaggtagaa ggcggcggcg aggatcagca
     1861 ggaactggaa gcccgtcacg agcgacaggt actcggccac gccgcccacc atcgccccca
     1921 gcaggttcgc gccgtagtcg atcgtcacgt cgcccgagcg cttgatgctg gagccgaaca
     1981 gcagcccggc gcagaagatg gggctgaaga ccatcacgct gtagacgagc gactccaggg
     2041 cgcggctctc gaacccgacg gccccgaccg gcacgaggta gttcagcgcc agcagcgcgc
     2101 cgagcaccgc gccgaccagc cacggccgcc tgatctcgac gcgggccacg acccagttcg
     2161 cgagcagcgc catgaccagc accgacgaga tgatcaggga ggccaccacc caggtcgatc
     2221 cccacagcag ggcgaactgg atgatcgact tcgactccag cagcatgaag cccgcgccga
     2281 ggaagaagaa ctcccaggac cacttccgcg ccgcgccgcg cacaacgccg aagaccgcga
     2341 cggccgacac gagcagcacc acggcgagcg cgctcgtgta gtgcgacgga aggtgcgggc
     2401 ggcgcatgta gaggaagggc cagtcgtcgg tggccggcgc caccgcccgg tcgcgctcgt
     2461 gcctcacggg gggcgcgagc ggcgatccct ggttgaacgc ggtcacctcg gacggcgggt
     2521 cggggtagcg cgccagcgtc ctgagccgcg gccccacgac gatgacgccg agctgatcct
     2581 tcgccgggtg cacgtgcacc atgggctcca tgccgaaggc ggcggcggcc gtattcgcca
     2641 ggcgatctac gagccagggc tcgcggaagt agttgtagac gacgagcacc ccgtctggag
     2701 cgaggacgtc ccgcacggcg cggaaggact cctcggtgaa catgtagctc tcgaggcgca
     2761 cgcccgagaa ggccgactgc agcgtcagcg agtcgatcag cgcgaacacc accaggtcgt
     2821 acttcttcgt cgtcgtccga aggtagtgcc gggcgtcgtc ggcgaccgcg tgcacgcgcg
     2881 ggtcggcgta gggccgatcg gggtggccct gccgcccgag ccggaggatg gccggatcga
     2941 tctcgacggc gtccacgctg gccacgccgt gcgccagcgc ggccgccacg tcggtgcccg
     3001 tgcctgcgcc caggatgagc gcgtcatgga aggtgtcgcc gaaggccatg tagggccact
     3061 ggtagaaata ctccttggcg ttcaccctgg ccatggactg gtgccagatg ttgttcacct
     3121 ccacgaccgt ctcggggccc gcgcgcttca gggtgatctt gtagtagggg gaccacaggc
     3181 tgccgcgctg catgtcgtag accaggccgg cgcccgtcac caggcacagg accgccgcga
     3241 gcgaggcagt ggtccagcgg cgggcccgca ggaacgggac ggcggcggcg agagacgcga
     3301 tgaaccaggc gctcggcggc gcttcgagcc acgacatcag ggcgaaggcc gccgtcccgg
     3361 ccaggctgcc ggccaggttg agcgtgtacg cgcgcagcgg cgggagcgcc gtcatctcgg
     3421 cggccatgcg ctgggccagc gccgcgaaca acgccgccac ggcgacgaag atcacgggca
     3481 gcagcagcgt cgtctcgacg gcggtgaccg ggtcgctcgt gccgctggtg aagtagatgc
     3541 tgccgggggc gttgatccgc acctccaggc ggaagaagta gacgacggcg acgaggagca
     3601 gctggagcgc cggataccag gcgaacagcg agcgcgccgc gcgaccgaga agcgagccga
     3661 tgccgatccc gaggaaggct gccagcagga tgaagttcga gaagtaggag agcaggcgga
     3721 tctgggccgg cagccagcgg atcagcgcga cctcgaggaa cagcacgagg aacgacgcga
     3781 ggaagatgcg cagcggagcc gggaacatcc ggacatctta ccccgcccgc gccgcgccgg
     3841 ctcggacggc cggccggcgc ggcgcctccg ggctacgcga ggctgcccag gcggacgagg
     3901 gcgagcacgg gccgccccgc ttcccgcgac gcggccacgt cgacgcggaa ctccgccacc
     3961 cagtcgttgt gcatgtcggg atcgacgagc atctgctcga cccggagcgc ctcgccctcc
     4021 ggcgcgtcgg acacgtgggt gtgccgcagg ttccgcgcct cgggatcgaa gcggagcggg
     4081 ccgtgctcgg cgcgatagcc ggccctggcc gcctcgaggc ggtccgcggt ccactcgccg
     4141 ccgccgtcgc cggtcccatc gagcgccgcc agcgcgccgg cgtagtcgcc gacccaccac
     4201 gcgcggagga acgtgaagag gcgcgcgcgc acggccgcca ggaaggcctt ccgatcgcgc
     4261 gtgacgtcct cgcgggccgc ggcgggcccg ggcggcttga cgtccgggcc gggccgggct
     4321 ccggcgccgt gcggctggta gtccggatcg cgcatgcgtt cccactc
//
LOCUS       DAOSIF010000246         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17828_length_4350_cov_3.465425, whole genome shotgun sequence.
ACCESSION   DAOSIF010000246 DAOSIF010000000
VERSION     DAOSIF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17828_length_4350_cov_3.465425"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLT35_08800"
     CDS             <1..560
                     /locus_tag="PLT35_08800"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG29365.1"
                     /translation="CLAGPVVAAAVILRRDSPIARLADSKLVPPRERERLSALIRARA
                     LAWAIAEVGPADIDRMAIHHASIEAMRRAALRLAPLPDVVLVDAFRIPGLPFAQRGVV
                     HGDRLCASIAAASIVAKAHRDDLMRALDAADDRYGYARHKGYATPQHLAAIARFGYSP
                     EHRRSFRPASLFGKIGAGNRLADPV"
     gene            573..>1962
                     /locus_tag="PLT35_08805"
     CDS             573..>1962
                     /locus_tag="PLT35_08805"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29366.1"
                     /translation="MNRDETLRRAEKLLRQGRVEAAIPEYARLVEASPRDWNTANLLG
                     DLYVRAGRIDEAVGHYARIAEHLASEGFVSKATALYKKIVKIRPDDDAALLRTAELSA
                     SQGLTAEARVHLRALFAQRARRGDRAGASRAATALADLDPRDAAARAEIARMMADLGD
                     AADAAAHLRAAGHAWLAAGQADLAAKAWREGLSLDPANEALAGDLLETLLAQGDLDGA
                     RDVAKTAGHWRAVADGFTNAGRTGDACAALERVLASEPADVPARIQLARAAIAEQDAA
                     RARALLAPVADNRDPAVLLGLAEIEFLSGSVEVGRAALERLLAASPELVQAAVEVGCD
                     IGRTAPDKGFAVIGVAVKDAEAAGDTDAALEMIERFLAAAPGCIQALEDYIRLCGQGF
                     YEHQRYRAQVQLADAYLSVGRWREARALAEALASARPDDSTHQRRLALASAGLGAAAG
                     GEILPQSAPSPAE"
     assembly_gap    1963..1972
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1973..2817
                     /locus_tag="PLT35_08810"
     CDS             <1973..2817
                     /locus_tag="PLT35_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29367.1"
                     /translation="PGEPDAPSAALVEDETLADVAVAPALWTMPSTEPVPALIVPPKP
                     VAGAARAEDQPDALDFEAVIELTDEVVAPDAGSRLRTAEPEVFEIDLSGELDELMASN
                     GGIAGRPGGRGPSLGPAGGGHPASLEGIEGYFGRLREQSSRDAEMAEAARAYDLASEH
                     FNRGEIAEAEGCLRTAARDPLFRFRAASMLARIARDRGSLAEAIEWLERAGEALAPSE
                     EASRSLLYELGDTLESAGEEARALAVFLGLASSAPTYRDVGARLARLRERAAGGGEPW
                     RSPR"
     gene            2814..3083
                     /locus_tag="PLT35_08815"
     CDS             2814..3083
                     /locus_tag="PLT35_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29368.1"
                     /translation="MSVLSRLVFMAYFVEAGLVLLVVPWSPLWERNYFVQAWPALAGV
                     AHSSLCRGAVSGLGIVSLWAAMAELSALIGMRAAGVARESNPRRN"
     gene            3155..4057
                     /locus_tag="PLT35_08820"
     CDS             3155..4057
                     /locus_tag="PLT35_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOG29369.1"
                     /translation="MSAAEEGAADFGTSLRRAREARGLTLQDLAVTTKISASVLGALE
                     RNEPGKLPGGIFSRAFVRAYAREVGLDPESTVARFVAAFPADSGASGIPPPALADAEG
                     FERHRRTAAAAVRILGAAAIVVALGLMGFAWWQRSGRTEEAADARAPVALDGSLGSPA
                     SIVGEAPPAAPASPGPQSDTTPSADDAPLPVPSERAAGLPTSQPDGTQAAGQHGAPLV
                     VGLRAEGECWLSVTVDGTTQPQRTLGPGEHAEFRVRKLLTMRIGNAGAIAMTLNGAPA
                     KPLGRAGEAVTATIPADGFGSLLR"
     gene            4065..>4350
                     /locus_tag="PLT35_08825"
     CDS             4065..>4350
                     /locus_tag="PLT35_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29370.1"
                     /translation="MDSLYRTPLLDCFKRGDIAPDIRMMAAEGILAPRAHEQLGLLVL
                     LADDPDPGIRAATESTLRRLPVRDLARFLGRGDLAPEIVAFFKARGVEPER"
BASE COUNT          568 a         1554 c         1611 g          607 t
ORIGIN      
        1 ggtgcctggc agggcccgtc gtggctgcgg ccgtcatcct gcgccgggat tcgccgattg
       61 cgcgcctcgc ggattccaag ctcgtgcccc cgcgcgagcg cgagcgcctg agcgcgctca
      121 tccgcgcccg ggcgctggcg tgggccatcg ccgaggttgg ccccgccgac atcgaccgga
      181 tggcgatcca ccacgcgtcg atcgaggcga tgcggcgcgc ggcccttcgg ctcgcgcccc
      241 tgccggacgt cgtgctcgtg gacgccttcc gaatcccggg ccttcccttc gcccagcgcg
      301 gcgtcgtgca cggcgatcgc ctgtgtgcct cgattgccgc cgcgtccatt gtcgccaaag
      361 cccaccgcga cgatctgatg cgggcgctcg atgccgccga tgatcggtac gggtacgcgc
      421 ggcacaaggg ctacgcgacg ccccagcacc tggcggcgat tgcgcgattc gggtactccc
      481 cggagcaccg ccgctcgttc cggcccgcat cgctgtttgg taagatcggc gcgggcaatc
      541 ggctggccga ccctgtctga cacgttcggc acatgaaccg cgacgagacg ctccgacggg
      601 ccgagaagct gctgcgccag gggcgcgtcg aggccgcaat tcccgagtat gcgcggctcg
      661 tcgaggcttc tccgagggac tggaacaccg ccaaccttct gggcgacctc tacgtcaggg
      721 ccggccggat cgacgaggcc gtcggccact acgccaggat tgcggagcac ctcgccagcg
      781 agggcttcgt ctcgaaggcc acggcgctct acaagaagat cgtcaagatc cgccccgatg
      841 acgatgcggc gctgctccgg acggcggaat tgtccgccag ccaggggctg accgcagaag
      901 cccgcgtgca cctgcgcgcg ctcttcgcgc agcgcgcccg gcgcggggac cgggccggcg
      961 cgtcacgggc cgcgacggcg ctggcggacc tggatccccg tgatgcggcg gcgcgcgcgg
     1021 agatcgcccg gatgatggcc gacctcggcg acgctgccga cgccgcggcg cacctgcgcg
     1081 cggccggaca cgcgtggttg gcggcgggcc aggccgacct cgcggcgaag gcctggcgcg
     1141 aggggctgag cctcgatccg gccaacgaag ccctggccgg cgacctgctg gagacgctgc
     1201 tcgcccaggg ggatctggac ggagcccgcg acgtcgcgaa aacggcgggc cactggcgcg
     1261 cggttgcaga cggcttcacc aacgcgggcc ggactggcga tgcctgcgcc gccctcgagc
     1321 gcgtgctggc gtccgagccc gccgatgtgc ctgcccgcat ccagctcgcc cgcgcggcga
     1381 ttgccgagca ggacgcggct cgggcacgcg ccctgctggc cccggtcgca gacaatcgag
     1441 acccggccgt gctgctcggg ctcgccgaga tcgagtttct ctccgggtcc gtcgaggtcg
     1501 gccgcgctgc tctcgagcgg ctgctcgcgg ccagcccgga gctggtgcaa gcagccgtcg
     1561 aggtgggctg tgacatcgga cggaccgcgc ccgacaaggg cttcgctgtc atcggcgtcg
     1621 ccgtcaagga cgccgaggcc gccggcgaca cggacgccgc cctcgagatg atcgagcgct
     1681 tcctcgcggc cgctccgggg tgcatccagg ccctcgagga ctacatccgg ctctgcgggc
     1741 agggcttcta cgagcaccag cgctaccgcg cgcaggttca gctggccgac gcgtacctct
     1801 cggttggacg atggcgggag gcgcgggcgc tggcagaagc gctcgcgagc gcgaggcccg
     1861 atgacagcac ccaccagcgg cggctggccc tcgccagcgc cggcctcggc gcggcggccg
     1921 gtggcgagat cctgccgcag tccgcgccca gccccgctga ggnnnnnnnn nnggcccggc
     1981 gagccggacg ctccgagcgc cgccctcgtc gaagatgaga cgctggccga cgtcgccgtg
     2041 gcccctgccc tctggacgat gccttcgact gagccggttc ccgcgctgat cgtgccgccc
     2101 aagccggtgg cgggcgcggc gcgggccgaa gaccagcccg acgccctgga cttcgaggcg
     2161 gtcatcgagc tcaccgacga ggtggtggcg cccgacgccg ggtcgaggct gcgtaccgcc
     2221 gagcctgaag tgttcgaaat cgatctgagc ggggagctcg atgagctgat ggcctccaac
     2281 ggcgggatcg ccggccggcc cggaggcagg gggccttcgc tcgggcctgc gggaggcggg
     2341 caccctgcca gtctcgaggg gatcgagggg tacttcggga ggctccggga acagtccagc
     2401 cgagacgcgg agatggccga ggcggcccgc gcgtacgacc tcgccagcga gcatttcaat
     2461 cgcggcgaga tcgcggaggc cgaaggctgt ctccgcacgg cggcgaggga cccgcttttc
     2521 aggttccgtg cggcctcgat gctcgcgcgg atcgcccggg atcgcggaag cctcgccgag
     2581 gcgattgagt ggctcgagcg ggccggcgag gccttggcgc cgtcggagga agcatcccga
     2641 agcctgctgt acgaactcgg cgataccctc gaatcggcag gcgaggaagc cagggcgctc
     2701 gcggtcttcc ttggtctggc gtcgagcgcg ccgacctacc gcgacgtcgg cgcgcggctg
     2761 gcgcgcctgc gggagcgggc ggccggcggc ggcgagccgt ggaggagccc gcggtgagcg
     2821 tgctgagccg cctggtgttc atggcctact tcgtcgaggc tggcctcgtg cttctcgtcg
     2881 tgccgtggtc gccgctctgg gagcgcaact acttcgtgca agcctggccg gcccttgccg
     2941 gcgtcgcgca cagcagcctc tgccgcggtg cggtctcggg tctgggcatc gtgagcctct
     3001 gggcggcgat ggccgagctg tcggcgctga tcggcatgcg tgcggccggc gtggcgcgcg
     3061 aatcgaaccc gcgccgcaat tgacagtgca tacggcggtt ccctaacatg gtggccccat
     3121 gtcggctggc gatcgtccgc aactgagact gggcatgtct gcggcggagg agggcgccgc
     3181 cgacttcggg acctccttgc gccgcgcgcg cgaagcgcgc ggcctgacgc tccaggatct
     3241 ggcggtcacc acgaagatct cggccagcgt cctcggggcg ctcgagcgca acgaacccgg
     3301 caagctcccg ggcggcatct tctcgagggc gttcgtgcgt gcgtatgcgc gggaggtggg
     3361 actcgatccc gagtcgaccg ttgcgaggtt cgtcgcggcg tttcccgccg actccggcgc
     3421 ttcgggcatt ccgcctcccg ctcttgccga cgccgagggc ttcgagagac acaggcggac
     3481 ggccgcggcg gcggtacgga tcctcggcgc cgccgccatc gtcgtcgcac tcgggctgat
     3541 ggggtttgcg tggtggcagc ggagcggccg gacggaagag gcggccgacg cccgggcgcc
     3601 cgtggcgctc gacgggagcc tcggctcccc ggcatcgatc gtcggcgagg cgccgcccgc
     3661 cgcgcccgcc tcgcctggtc cccaatccga caccacaccc tcggccgacg acgcgccgct
     3721 gccggtcccg tccgagaggg cggccggact cccgacatct cagccggacg gaacgcaggc
     3781 cgccggacag cacggcgcgc ccctggtggt cggtctgagg gccgaggggg agtgctggct
     3841 ctcggtcacc gtcgatggaa cgacgcagcc gcagcgcacg ctgggcccag gcgagcacgc
     3901 cgagttccgg gtccggaagc ttctgaccat gcggatcggc aacgccggcg cgatcgcgat
     3961 gaccctgaac ggcgctccgg caaagcccct cggccgcgct ggcgaggcgg ttaccgcgac
     4021 gatcccggcg gacggcttcg ggagcctgct ccgctaggcc ccggatggat tcgctttacc
     4081 gcacgccgct cctggactgc ttcaagcggg gggacatcgc gcccgacatc cgaatgatgg
     4141 cggccgaggg gatcctcgcg ccgcgcgccc acgagcagct ggggctgctg gtgctgctcg
     4201 cggacgatcc cgaccccggc atccgcgccg ccacggagtc cacgctccgg cggctgcccg
     4261 ttcgcgatct cgcgcgattt ctcgggcgcg gcgatctggc gccggagatc gtggcgttct
     4321 tcaaggcgcg cggtgtcgag cccgagaggg
//
LOCUS       DAOSIF010000247         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17836_length_4348_cov_2.875844, whole genome shotgun sequence.
ACCESSION   DAOSIF010000247 DAOSIF010000000
VERSION     DAOSIF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17836_length_4348_cov_2.875844"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLT35_08830"
     CDS             <1..230
                     /locus_tag="PLT35_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG29371.1"
                     /translation="DITKVKAELAVDAVFDTMRLSMQRGDRIELRGFGVFQVKPRKRG
                     IGRNPRTGKEVRIPPGRTIRFKPGKDLQHLG"
     gene            261..1247
                     /locus_tag="PLT35_08835"
     CDS             261..1247
                     /locus_tag="PLT35_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG29372.1"
                     /translation="MTDSSTPPHAAAGPFQQPAREVAGIRVAAPRRPRERYALHALLL
                     TATFLTTTAAGALHWSAFESDFGVREASSSMSALAIRGLWYSITLLAILGAHELGHYF
                     ACGYHGVRASLPFFLPVPIPLTGTAGAFIRIRQPITAKSVLFDVGVAGPLSGFLVAVP
                     ALFLGLARSRVVPLPAEFVGAELGEPILFKLASWAVWGPIPEQLSLNLHPMAFAAWFG
                     LLVTAINLFPIGQFDGGHIAYAVFGRRSFYVSAGTVGVAVALCFYSSSWIAWTALAIV
                     ILSVFGWRHPPTWDEGVPLDRARIRLAVITLVVFALCFTPAPIEPLDLVTAR"
     gene            complement(1254..3773)
                     /gene="priA"
                     /locus_tag="PLT35_08840"
     CDS             complement(1254..3773)
                     /gene="priA"
                     /locus_tag="PLT35_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG29373.1"
                     /translation="MVRFASVAVPLPQVDLLTYRVPESLETPAIGARVLVPVGSRILT
                     GCVAALHEEAGEIGRDGGPTVRDLIDVLDGDAYVPGRILDLAAWVAEYYACGPGEAVA
                     AAVPRSAWIESRQFVRATDEGRRALSGDAGVPLGEPARRLIAAAAAAGGLPASALRAR
                     LDRKPAERGVGRFPLASVLRTLGRAGLVTVSQVLEGSRDPSKTERVLELTAAGREAAT
                     AQAGAKPTAFRLGPRQQALIGALRDAGAPMSAASLRQAGFDLGGLHRSGLRGLVAVRH
                     VRAERDPFAGAWGRAGRRQAEAPPVDLTAEQAAAVDRLGARVDAAAFHVALLHGVTGS
                     GKTEIYVRLAARADAAGRRAIVLVPEIALTPGTAAVFRARFGDRVAIQHSGLSDGERH
                     DQWQRTRRGEIAVVVGTRSAVFAPLADVGLIVVDEEHDASYKQDETPRYNGRDVAVVR
                     GRRESALVVLGSATPSMESYAHAKRGRYELVSLERRVLERPLAAVRTVDMRREYAEAG
                     PETVISRALASAMADTLARGEQVLLLLNRRGYSTSVFCRQCGAALECPSCSVSLVVHG
                     RGQAVCHYCNHSRRVPKACPACGGPFLEQAGCGTERVEAETRALAPEARVARLDRDTA
                     RRRGAAASLLSRFADGDIDVLVGTQMIAKGHDFPRVTLVGVISADVGLGLADFRAAER
                     TFQLLTQVVGRAGRGDAPGEAIVQTLCPEHYSIRLACRQEYAAFFDAEMAFRRAMRYP
                     PVVALVNGVVRGPTQAAAMQAAGAIVAAIRERGTERQFTVLGPAPAPLARLRGRHRAQ
                     FFLKGPAASRAAMRQAVLAALRARPDLRRHVTIDVDPASVL"
     gene            complement(3785..>4348)
                     /locus_tag="PLT35_08845"
     CDS             complement(3785..>4348)
                     /locus_tag="PLT35_08845"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG29374.1"
                     /translation="RETLDEIRADLGDCTRCKLHRLGRRQIVFGTGNPAAELMFVGEA
                     PGHDEDLQGVPFVGRAGQLLTKIIESIGCSREDVYIANVIKCRPPENRNPEPDEVASC
                     EPFMLRQVESIRPKVIVALGTFAAQALLRTSDPISRLRGRLLHYGAARLIPTFHPAYL
                     LRSPERKREVWEDMKNVRAILRGDTRA"
BASE COUNT          566 a         1610 c         1520 g          652 t
ORIGIN      
        1 ccgacatcac caaggtgaaa gccgagttgg cggtggacgc cgttttcgac acgatgcgcc
       61 tctcaatgca gcggggcgac cggatcgaac tgcgcgggtt cggcgtcttc caagtgaagc
      121 cgcgcaagcg cgggattggc cgtaaccctc ggacaggcaa ggaagtgcgc atcccgccgg
      181 gccgcacgat ccgcttcaag ccgggcaagg acctccagca cctcgggtga tgggcccgct
      241 cccgcccccg gcctggtccc gtgactgact cctcgacgcc gcctcacgct gctgccgggc
      301 catttcagca gcccgcgcgg gaggtggcgg gtatccgagt tgccgcgcct cgaagacccc
      361 gcgagcgcta cgctctccac gcactgctgc tgacggccac gttcctgacg acgacagccg
      421 ccggtgcgct gcattggagc gcgttcgaat cggacttcgg cgtccgggaa gcctcgtcct
      481 cgatgagtgc gctcgcgatt cgcgggctct ggtactcgat tacgctgctg gcgatcctcg
      541 gtgcacacga actcggccac tacttcgcgt gcgggtacca cggcgtgcgc gcctcgctcc
      601 cctttttcct gcccgtcccg atcccgctga ccggcacggc cggcgccttc atccggatcc
      661 gccagccgat caccgccaag tccgtgctct tcgacgtcgg cgtggccggc ccgctctcgg
      721 gctttctggt ggccgtgccc gccctcttcc tcgggcttgc gcgctcgcgg gttgtcccgc
      781 tcccggcgga gttcgtcggc gcggagctgg gcgagccgat cctgttcaag ctggcgtcct
      841 gggcggtctg gggcccgatc ccggagcaac tgtcgctcaa cctgcatccg atggcgttcg
      901 cggcatggtt cggcctgctg gtgacggcca tcaacctgtt tccgattgga cagttcgacg
      961 gcgggcacat cgcctatgcg gtgttcggcc gccggtcctt ctatgtgagt gccgggacgg
     1021 tcggcgtggc agtcgcgctc tgcttctact cgtcgtcctg gatcgcctgg acggcgctcg
     1081 cgattgtcat tctatcggtc ttcggctggc gccatccgcc cacctgggac gagggcgtgc
     1141 cgctggatcg cgcgcgaatc cgtctcgcgg tcatcacgct ggtggtgttc gcgctgtgct
     1201 tcacgccggc gcccatcgag cccctggacc ttgtgacggc caggtaggcc cttctagagg
     1261 acgctcgcgg ggtcgacgtc gatcgtgacg tggcggcgga ggtccggcct cgcgcgaagc
     1321 gccgcgagaa cagcctgacg catggcggcg cgcgacgctg ccgggccctt gaggaagaac
     1381 tgggcgcggt gccggcccct cagccgcgca agaggggccg gcgccgggcc gagcacggtg
     1441 aactgccgct cggtgccccg ctcccgaatg gccgccacga tggcgcccgc ggcctgcatc
     1501 gccgcggcct gagtgggtcc acgcacgacc ccgttaacga gggccacgac gggcgggtac
     1561 cgcatcgcac gccggaatgc catttccgcg tcgaagaagg ccgcgtattc ctgccggcac
     1621 gcgagccgga tgctgtagtg ctcggggcat agcgtctgga cgatggcttc gcccggcgca
     1681 tcgccgcggc cggcgcgccc caccacttgc gtcagcagct gaaacgtccg ctcggcggcc
     1741 cgaaagtcgg cgaggcccag gccgacgtcg gccgagatta cgccgacgag ggtcacgcgc
     1801 gggaagtcgt gcccctttgc aatcatctgg gtgccgacga ggacgtcgat gtcgccgtcg
     1861 gcgaacctcg acaggaggct ggccgccgcg ccgcgccgcc gcgccgtgtc gcggtcgagg
     1921 cgggccacgc gcgcctcggg cgccagggcg cgggtctccg cctcgacgcg ttccgtgccg
     1981 cagccggcct gctcgaggaa cggcccgccg caggcgggac acgccttcgg cacgcggcgg
     2041 ctgtggttgc agtagtgaca caccgcctgc ccccggccgt gaacgaccag cgacacggag
     2101 cagctcggac actccagcgc ggccccgcat tgtcggcaga acaccgaggt cgagtacccg
     2161 cggcggttca ggagcagcag cacctgctcg ccgcgcgcca gcgtgtcggc catcgcggat
     2221 gcgagcgcgc ggctgatgac cgtctcgggg ccggcttcgg cgtattcccg gcgcatgtcc
     2281 accgtgcgca cggcggccag cgggcgctcg agcacccggc gctcgaggct gaccagctcg
     2341 taccggccgc gcttcgcgtg cgcgtagctc tccatcgaag gcgtcgccga gcccaggacg
     2401 accagggccg attcgcgccg cccccgcacg accgccacgt cgcgcccgtt gtatcgcggc
     2461 gtctcgtcct gtttgtagga ggcgtcgtgt tcttcgtcca ccacgatcag gccgacgtcg
     2521 gcgagcggcg cgaagacggc cgacctggtg ccgacgacga cggcgatctc gccgcggcgg
     2581 gtgcgctgcc actggtcgtg ccgctcgccg tccgacaggc cgctgtgctg gatggcgacg
     2641 cggtcgccga accgggcgcg gaacaccgcc gccgtgcccg gcgtcagcgc tatctccggc
     2701 acgagcacga tagcgcggcg gccggcggcg tccgcccgcg cggcaagcct gacatagatc
     2761 tccgtcttgc cgctcccggt cacgccgtgc agcagcgcga catggaacgc cgccgcgtcg
     2821 acgcgcgcgc caaggcggtc taccgcggcg gcctgttcgg ccgtgagatc gacgggcggc
     2881 gcctcggcct ggcggcgccc cgcacggccc caggccccgg caaacgggtc gcgctcggct
     2941 cggacgtgcc gcacggcgac gagtccgcga agcccggagc ggtgcaggcc tcccagatcg
     3001 aagccggcct gcctcagcga agccgcgctc atcggcgccc cggcgtcgcg cagggcgcca
     3061 atgagcgcct gctgcctcgg ccccagccgg aaggccgtcg gcttcgctcc agcctgcgct
     3121 gtcgctgcct cccggccggc cgccgtcagt tccagcactc gctcggtctt cgacgggtcc
     3181 cggctccctt cgagtacctg gctgacggtc accaggcccg ctcggccgag cgtgcgcagg
     3241 acgctggcga gaggaaacct cccgaccccg cgctcggccg gcttccggtc gagccgggct
     3301 ctcaacgccg acgccggcag cccgcctgcc gctgccgccg ccgcgatcag ccgccgggcc
     3361 ggctcgccaa gcggcacccc ggcgtcaccc gagagcgccc gacggccctc gtcggtcgcc
     3421 cgcacgaact gccggctctc gatccaggcc gagcgcggca cggcggccgc cacggcctcc
     3481 ccggggccgc acgcgtagta ctcggccacc caggcggcca ggtcgaggat cctgccgggc
     3541 acgtaggcgt cgccgtcgag cacgtcgatg aggtcgcgca cggtcggccc cccgtcccgt
     3601 ccgatctcgc cggcctcttc gtgcagcgcc gccacgcagc cggtcaggat tcgcgaaccc
     3661 acgggcacga ggacgcgcgc gccgattgcc ggcgtctcga gcgactcggg gacgcggtac
     3721 gtgaggagat cgacctgcgg aagcggaacg gcgacggacg cgaagcggac catggctcgg
     3781 tgggctaggc ccgggtgtcg ccgcgcagga tggcgcggac gttcttcatg tcctcccaca
     3841 cctcgcgctt ccgttcgggg ctccgcagca ggtacgcggg gtggaacgtc gggatgagcc
     3901 tcgcggcccc gtagtggagc agcctgcctc ggaggcgcga gatcggatcg cttgtccgca
     3961 gcagggcctg tgccgcgaac gtgcccagcg ccacgatcac cttcggccgg atcgactcga
     4021 cctgccgcag catgaagggc tcgcagctgg cgacctcgtc aggctccggg ttgcggtttt
     4081 cgggcggccg gcacttgatc acgttggcga tgtacacgtc ttcgcggcta cagccgatgg
     4141 attcgataat cttcgtcagc agttgcccgg cccggccgac gaacgggacg ccctgcaggt
     4201 cctcgtcgtg ccccggggcc tccccgacga acatcagctc cgcggcggga ttgccggtcc
     4261 cgaacacgat ctggcgccgg cccagccggt ggagcttgca gcgggtgcag tcgccgaggt
     4321 cggcccggat ctcgtcgagt gtctcgcg
//
LOCUS       DAOSIF010000248         4338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17881_length_4338_cov_3.477002, whole genome shotgun sequence.
ACCESSION   DAOSIF010000248 DAOSIF010000000
VERSION     DAOSIF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17881_length_4338_cov_3.477002"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLT35_08850"
     CDS             complement(<1..564)
                     /locus_tag="PLT35_08850"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOG29375.1"
                     /translation="MPRNAEVIRQWKILRSIEAARCGLTVAELAASARVVTRTIWRDL
                     AALQEAGFPLVDESRDNRTYWKINQSPFKHLTQLGLSLSELCALYLSRRLVESLVGVP
                     FQTALGDAFAKFERDLPERMKQYLDRLPALMTARPATGRLSLPKGHDRMIEQLVDASV
                     ALRHVRMRYYSLSHDREADYLVYPLRFV"
     gene            complement(625..1503)
                     /locus_tag="PLT35_08855"
     CDS             complement(625..1503)
                     /locus_tag="PLT35_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOG29376.1"
                     /translation="MALRILAVGDIAWDIFVRPEGDLVRGSDALGTVDVLPGGAAANV
                     AVWARRLGADVTLVGKIGDDTLGALMLAHLRSEGVASHVITVPRSATTRVGILVAQDG
                     EHSFVIDHTKVLHFGEEDAPPGLLDGADAVYFNGYDIFLARSTAFLEPLLAAARRRGI
                     PLAFDPSSFALIDAYGASRLLDGVGPLDVLLLNEAEAEALRRGTGAELTTRASLVVVK
                     RGPGGASAHAAGAAWSAGAERVAVVDTTGAGDAFDAAFLVEWLRTRDVEAALSAGNRL
                     GAWVAGHLGAQPPLPG"
     gene            complement(1526..1945)
                     /locus_tag="PLT35_08860"
     CDS             complement(1526..1945)
                     /locus_tag="PLT35_08860"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG29377.1"
                     /translation="MAIHSARAVWKGNLADGNGVMTLPKGAYEGPFTRASRFETGPGT
                     NPEELIGAAHAGCFSMYLSAILSASGFPPVEIRTTATVHLGDGPAITLIELDSEAQVP
                     GCDEAVFLEHAEKAKAGCPVSKALSAVPMTLKAKLVK"
     gene            complement(2070..3275)
                     /locus_tag="PLT35_08865"
     CDS             complement(2070..3275)
                     /locus_tag="PLT35_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG29378.1"
                     /translation="MTEADALTVLCLASYEKGEEFMRECHRLGCRVLLVTLESLRNAA
                     WPREAITETFYMPRDLGREDLLKGVSHIARTRRLDRIVALDDFDVETAALLREHLRVA
                     GMGETTARYFRDKLAERMKARSAGIPVPDFVHVLNDEAIRAFAAAVGPPWILKPRSQA
                     AAMGMKRVDRLEDLWPAIDSLGDRRSFYLLERFVPGDVFHVDAIVRGRAVVFQAMHRY
                     GQPPFEVAHAGGVFTTMTVPKHDPAWSALAAMNRDVLALFGMVRGVAHTEFIRNAADG
                     VLLFLETSARVGGAHIVDLVEAETGLNLWREWARLEVAGEDGAYDPPPLADRHAGLVL
                     TLARQERPDLSAYADPEIVYRVKKPHHAGLIVASADQARVRALVEEYASRFTADFFAF
                     APAPEEPPE"
     gene            3396..4208
                     /locus_tag="PLT35_08870"
     CDS             3396..4208
                     /locus_tag="PLT35_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOG29379.1"
                     /translation="MTASDVVARPVGKPLAGDIRVLAGVTSPELGNTRDVLVYLPPSH
                     QRGGGPFPVVYMHDGQNLFDPVTSFAGEWRVNETLERLAGEGIEAIAVAIPNMGMERV
                     DEYSPFRDERRGGGRGDRYLEFVVRTLKPEIDRRFRTRPDRACTGIMGSSMGGLISLY
                     GFFRHDTVFGFAGVMSPSLWFGGGAIFDVVKNSRAPWSGAIYLDIGTGEERRAVRDAR
                     AMARLLRRRAGGPRHGLMYVEERGAAHHESAWAGRFERAARFLISPCAGRPH"
BASE COUNT          642 a         1527 c         1529 g          640 t
ORIGIN      
        1 cacgaagcgc aggggataca cgaggtagtc ggcctcccgg tcgtgcgaga gcgagtagta
       61 ccgcatccgc acgtgcctga gggcgaccga ggcgtccacc agttgctcga tcatccggtc
      121 gtgccccttc ggcagggaga gcctgccggt ggcgggcctc gcggtcatga gggccggcaa
      181 ccgatccagg tactgcttca tgcgctcggg caggtcgcgc tcgaacttgg cgaacgcgtc
      241 gcccagggcc gtctggaagg ggacgccgac gagcgattcg acgagccgcc ggctgaggta
      301 gagggcgcac aactcggaga gcgacagccc aagctgagtg aggtgcttga aggggctctg
      361 gttgatcttc cagtacgtgc ggttgtcccg cgactcgtcg acgaggggaa accccgcttc
      421 ctgcagcgcg gcgaggtcgc gccaaatcgt ccgcgtgacc acccgggcgc tcgcggccag
      481 ctcggcgacc gtcagcccgc agcgcgcggc ctcgatggac cgcaggatct tccactggcg
      541 gatgacttct gcgtttcgcg gcatcggcgc cccccttcac cgacccgatc cagccgcgtg
      601 aacgcggcgc gccgccgact gggctcagcc cggcagaggc ggctgggcac cgaggtggcc
      661 cgccacccac gccccgagcc ggttgcccgc gctgagcgcg gcctccacgt cgcgcgtccg
      721 cagccattcg acgagaaaag ccgcgtcaaa cgcgtctccc gctcccgtcg tgtcgacgac
      781 ggcgactcgt tcggccccgg cggaccaggc ggcgccggcc gcgtgggccg acgccccgcc
      841 gggcccccgc ttgacgacga ccagcgacgc ccgcgtcgtc aactcggcgc ccgtcccgcg
      901 gcgcagcgcc tcggcttcgg cctcgttcag gagcagcacg tcgagcgggc cgacgccgtc
      961 gagcagccgc gaagctccgt atgcgtcgat gagcgcgaac gacgacgggt cgaaggccag
     1021 cggaatgccg cggcgcctcg ccgcggccag cagcggctcg aggaacgccg tcgatcgcgc
     1081 aaggaagatg tcgtagccgt tgaaatagac ggcgtcggcg ccgtcgagca ggcccggcgg
     1141 ggcatcctcc tcgccgaagt gcagcacctt cgtatggtcg atgacgaacg agtgctcgcc
     1201 gtcctgagcg acgaggatgc ccacgcgggt ggtcgcagag cgcggcacgg tgatgacgtg
     1261 gctcgcgacg ccctcagacc gcagatgggc gagcatgagc gcgccgagcg tgtcgtcccc
     1321 gatcttgccg acgagcgtga cgtcggcgcc caggcgccgc gcccacaccg cgacgttcgc
     1381 ggcggccccg cccggcagga cgtccaccgt gccgagcgcg tcggagccgc ggaccaggtc
     1441 gccctcggga cggacgaaga tgtcccaggc gatgtcgccg acggccagga tccggagtgc
     1501 cacggctggt tcgcgccggc cctcgctact tcaccaactt cgccttcagc gtcatcggca
     1561 cagccgatag ggccttcgac accgggcatc ccgccttcgc cttctcggcg tgctcgagga
     1621 acacggcctc gtcgcagccg ggcacctggg cctcggagtc gagttcgatg agcgtgatgg
     1681 ccggaccgtc gccgaggtgc accgtggcgg tggtccggat ctccacaggc gggaacccgc
     1741 tcgccgagag gatcgccgaa aggtacattg agaagcagcc tgcgtgcgcc gccccaatga
     1801 gttcctccgg gttggtgccg ggccccgtct cgaaccggga cgcccgcgtg aaagggccct
     1861 cgtaggcgcc ctttggcagc gtcattaccc cgtttccgtc ggccaagttt cctttccaaa
     1921 ccgcacgcgc gctgtgaatg gccatgatcg gtctgctcct ttgtaacgtc cgcccagggc
     1981 ggagggcccg gcctgggcag tgggtgtcgc agtcgggtca cggcggcctg ccgctgatcc
     2041 ggtgcgccta agcatacaac gtcggcacgc tattccggcg gttcctcggg cgccggggcg
     2101 aacgcgaaga agtccgccgt gaatcgtgac gcgtactcct cgaccagggc gcggacgcgc
     2161 gcctggtccg ccgaagccac aatcagaccg gcgtggtggg gcttcttcac ccggtagacg
     2221 atctccggat ccgcgtaggc ggacaggtcg ggccgctcct gccgcgccag cgtcagcacc
     2281 agccccgcat gcctgtccgc cagcggcggc ggatcgtagg cgccgtcctc gcccgcgact
     2341 tcgagtctcg cccactcccg ccagaggttg aggcccgttt ccgcctcgac cagatcgacg
     2401 atatgggcgc ctccgacgcg cgcggacgtc tcgaggaaga gcaggacgcc gtctgccgcg
     2461 ttccggatga actccgtatg cgccacgccc cggaccatgc cgaagagcgc cagcacgtcg
     2521 cggttcatcg ccgcgagagc ggaccaggcc gggtcgtgct tcggcacggt catcgtcgtg
     2581 aagaccccgc cggcgtgcgc gacctcgaag ggcggctggc cgtagcggtg catcgcctgg
     2641 aagaccacgg cccggccgcg cacgatggca tcgacgtgga acacgtcgcc cggcacgaag
     2701 cgctcgagca agtagaacga cctccggtcg ccgagcgagt cgatggccgg ccagaggtcc
     2761 tccaggcgat cgacgcgctt catacccatg gccgcggcct gcgaacgcgg cttcaggatc
     2821 cagggtgggc ccacggcggc cgcgaacgcc cggatggcct cgtcgttcag gacgtgcacg
     2881 aagtccggca ccgggatgcc ggcgctccgg gccttcatgc gctccgccag cttgtcccgg
     2941 aagtaccgtg ccgtcgtctc tcccatgccc gccacgcgga ggtgttcgcg caggagtgcc
     3001 gccgtttcca cgtcgaaatc gtcgagggcg acgatccgat cgaggcggcg cgtccgggcg
     3061 atgtggctga cgcccttcaa caggtcttcg cgcccgagat cccggggcat gtagaaggtt
     3121 tccgtgatcg cctctcgagg ccaggcggcg ttcctgaggc tttcgagcgt cacaagcagc
     3181 acccggcatc ccagccgatg gcattcccgc atgaactcct cgcccttctc gtagctggcg
     3241 aggcagagca ccgtcagtgc gtcggcttcg gtcatggcgg cccccgtcgg ggatggagct
     3301 tccagcttac cccagaccag cggggcggct cgcgcgcgtt cttggccggc aggcggtctc
     3361 ccggctacga tgattgcttc gacagtcgaa ccggcatgac agcgagcgac gtggtagcgc
     3421 ggcctgttgg gaagcccctc gcgggcgaca tccgggttct cgccggcgtg acgagcccgg
     3481 agctcggcaa cactcgagac gtcctggtct acctgccgcc gtcccaccag cggggcggag
     3541 ggccgtttcc cgtcgtctac atgcacgacg gccagaacct gttcgacccg gtgacgtcgt
     3601 tcgccggcga gtggcgcgtc aacgagacgc tcgaacgtct cgcaggcgag ggcattgagg
     3661 caatcgccgt ggcgatcccg aacatgggca tggagcgggt ggacgagtac agcccgtttc
     3721 gggacgagcg gcgcggcggc ggccgcggag accgctatct cgagttcgtg gtccggacgc
     3781 tgaagcctga aatcgaccgc cgcttccgga cgcggcccga tcgggcctgc accgggatca
     3841 tggggtcgtc gatggggggg ctcatcagcc tgtacgggtt cttccgccat gacaccgtgt
     3901 ttggattcgc cggcgtgatg agcccgtcgc tgtggttcgg cggaggagcg atcttcgacg
     3961 tcgtgaagaa cagccgcgcg ccctggtccg gcgcgatcta cctcgacatc gggacgggcg
     4021 aagagcggcg ggccgtgcgg gacgcccggg ccatggcgcg gctcctcagg cggagggcgg
     4081 gcgggcctcg ccatggcctg atgtatgtcg aggaacgggg cgcggcgcac cacgagtcgg
     4141 cctgggcggg ccggttcgag cgcgcggcgc ggttcctgat ttccccctgc gccggtcgtc
     4201 cccactaggc cgcctccgcc gcctccatgc gggccagggg cggcagcctc gtcaacctcc
     4261 gcctcgccgg gccgcacgcg ccgcggctcg ggccggcagg cccccaggac gagcgggctg
     4321 gcggcgatcg ggccgctt
//
LOCUS       DAOSIF010000249         4337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17887_length_4337_cov_4.049043, whole genome shotgun sequence.
ACCESSION   DAOSIF010000249 DAOSIF010000000
VERSION     DAOSIF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17887_length_4337_cov_4.049043"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLT35_08875"
     CDS             complement(<1..937)
                     /locus_tag="PLT35_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG29380.1"
                     /translation="MRYQVAAAGLLLLSAYAGGAAQPLPVRAGRPAAAAVGGDLIFLD
                     ELVRELGPSADRARLQQGLGTAADLELLGRLVNIRLIVQEAATMGIAELPEIRKQVEV
                     GSREILREVLLEQLVKDVKADPAVVDSLVREAVREWKTTSLLFQDEAAARRARKEIAD
                     GASFADVASRAVAAKAAKTDGDSSYHARGDYLPAIAQAVAALKPGEVSPVVRIQAGFV
                     ILTVLDIRYPERPEARAEAQRQALSRAQVAFMTAREQDLKRKYVTVNEAVLKGLDYQA
                     PKPGLDALLKDPRVVARIKGAAPVTVADLTDYLRMQ"
     gene            complement(974..2074)
                     /locus_tag="PLT35_08880"
     CDS             complement(974..2074)
                     /locus_tag="PLT35_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29381.1"
                     /translation="MRCRHRLSPVAAVIPLVLLLGAWAMTPSAGTMAKVAVFPVENLT
                     SGSVPAAEVTRALVGGLTSAGVGVVSDDALEAFMARHRIRYAAGIDAAMAARLREETG
                     ADGAVFASMEWSSAAEPPKIALLARLASTSDAPTVVWADDVGLSGDDAPGLFELGLVN
                     DYQVLLARALDRLAGSLTAYLKTGRAAGAPKAASKFRPKTSHRQITLEPGRIYSIAVV
                     PFFNLSERRQAGQILALLFARHLAGRPEFRVVEPGVTRRQLLDARIIMDGGISLSDAD
                     AIAAATGADLVLGGRVFRYEDYEGPDGRTRVDFSTMLVDRRSGRVVWSSASDNEGTDG
                     VLVFERGTSRTAHAMATRMVRLVADRIAGRDR"
     gene            complement(2100..>4337)
                     /locus_tag="PLT35_08885"
     CDS             complement(2100..>4337)
                     /locus_tag="PLT35_08885"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOG29382.1"
                     /translation="LWTRVLTIAVGASVYGFTIILVAFLTGIALGSEAYGVFARASRA
                     AVPAARRVAAWFGATQIAVGVTALLVTVYLRDIPVAAVRLQGFVFGSEPGSFQARTWA
                     NFGLAFLYMVVPAFFMGAAFPLAGDVVARQRRAVGRGVGDVFASNTVGSILGAAVSGL
                     ILIRLVGIERSLQLLTVANAGLGLLVLASLRRTRWLPAGAAACTAAVLAFLASSPDAA
                     RIWDRKYFAIYRSNQPEAFRTPEMVREAVENTDVIYYSEGVESTVSVIRVKGGEQAFV
                     TNGRIEASTHLQAQQVQFTLGHLPMLLCERPSDVLVVGMGSGMTAGATAVHPGVDRVT
                     LVEIEGQVRGVARTFSAYNHNLLDHPKVSVVVNDGRNFLTTTNRTFDVITADPIHPWF
                     RGAGYLYSSEYFALAARHLRPGGVIAQWLPLYELRPEDLASVVKTVRQHFAHTMLWVT
                     HYDAVIVGRGSPFLVDEADLDRRIAAPGVAEDLRRVNMGSAADLLAYFLMGTEGMTRF
                     GRNGVLNTDDRLYLEFSAPFSMAAASVMRGNVEALAAHRESILPYLKPAGDAEGRRRQ
                     EEAAARQVAAGRMGDEALARYLAFGASDPRVGQALRRLTLEHPGFAPGRALWAEREAA
                     LALEPRLLQEAEFQLTTANALATTVRLSAVLVPVSRSRASIMFVDNRARAVYGQVYID
                     DYDRDGLAGRTAADVLAAVSAAYDQEAAAARERSQGLPPAEATLARFKAVIDGMVRRG
                     GRPGS"
BASE COUNT          649 a         1578 c         1495 g          615 t
ORIGIN      
        1 actgcatccg caggtagtcg gtcaggtccg cgacggtcac gggcgcggcg cccttgatcc
       61 gggcgacgac gcgcggatcc ttcagcaggg cgtcgaggcc cggcttgggc gcctggtagt
      121 cgaggccctt gagcaccgcc tcgttcacgg tcacgtactt gcgcttgagg tcctgctccc
      181 gggccgtcat gaacgccacc tgcgcccggc tcagcgcctg ccgctgcgcc tctgccctcg
      241 cctccgggcg ctccgggtac ctgatgtcga ggaccgtcag gatgacgaac ccggcctgga
      301 tccgaacgac gggactgacc tcgccgggct tcagggccgc caccgcctgc gcgatggcgg
      361 gcaggtagtc gccccttgcg tgatacgagc tgtccccgtc ggtcttcgcg gccttcgccg
      421 cgacggcccg cgaggcgacg tcggcgaacg aggcgccgtc ggcgatctcc ttgcgcgcgc
      481 gcctggcggc cgcctcgtcc tggaacagca gggacgtggt cttccactcc cggaccgcct
      541 ccctcacgag cgagtcgacg acggccgggt cggccttgac gtccttgacg agctgctcca
      601 gcaggacttc ccgcaggatc tcgcgggagc cgacctcgac ctgcttccgg atctccggaa
      661 gctcggcgat ccccatcgtc gccgcttcct ggacgatgag ccgaatgttc acgagacgcc
      721 cgaggagttc gaggtccgcc gccgtgccaa gaccctgctg gaggcgggcc cgatcggccg
      781 agggcccgag ttcccgaacg agctcgtcga ggaagatcag gtcgccgccg acggccgccg
      841 cggccggcct gccggcgcgg acgggcaggg gctgagccgc gccgccggcg taggctgaaa
      901 gaaggagcag tcccgccgcc gctacctgat accgcatgcg ctccttccgg gcggagacat
      961 ccgccgacgc cgcctaccgg tcacgtccgg ctatcctgtc ggcgacgagc ctgaccatcc
     1021 gggtcgccat ggcatgagcg gttctcgacg tcccgcgctc gaacacgagg acgccgtcgg
     1081 tcccctcgtt gtcgctcgcc gagctccaca cgaccctgcc gcttctcctg tcgaccagca
     1141 tggttgagaa gtccacccgc gtcctgccgt cgggcccctc gtagtcctcg tagcggaaca
     1201 cccgtcctcc cagcacgagg tcagcgccgg tcgcggcggc aatcgcgtcg gcgtcgctga
     1261 gggagatgcc gccgtccatg ataatccgtg cgtcgaggag ctgccgcctg gtgacgccgg
     1321 gctcgacgac gcggaactcc ggccgccccg ccaggtgcct ggcgaacagc agtgcgagga
     1381 tctgccctgc ctgccggcgc tcgctcaggt tgaagaacgg cacgacggca atcgagtaga
     1441 tccggccggg ctcgagcgtg atctgccggt gagacgtttt cgggcggaac ttcgacgccg
     1501 ccttcggggc cccggcggca cgccctgttt tcagataggc cgtaagcgac cctgcgaggc
     1561 gatcgagggc ccgcgccagc agcacctgat agtcgttcac caggcccagc tcgaacaagc
     1621 cgggcgcatc gtctccggac aggcccacat cgtcggccca gactaccgtc ggcgcgtccg
     1681 acgtggacgc gagcctggcg agcagggcga tcttgggcgg ctcggcggcg ctcgaccatt
     1741 ccatcgacgc gaacacggcg ccgtcggccc cggtttcctc gcgcagccgc gcggccatgg
     1801 ccgcgtcgat ccccgccgcg taccggatgc ggtggcgggc catgaacgcc tcgagcgcat
     1861 cgtccgagac gacgccgacc ccggcggacg tcaggccgcc cacgagcgct cgagtcacct
     1921 ccgccgccgg cacgctgccg ctcgtcaggt tctcgacggg aaacacggcg actttcgcca
     1981 tcgtgccggc agacggcgtc atcgcccatg ccccgagcag gagcacgagg ggaatcaccg
     2041 cggcgacggg cgagagccgg tgtctgcagc gcacctggga gtcctttctc cccgcacggc
     2101 taggagccgg gccgtccacc acgccggacc attccatcga tgacggcctt gaatcgcgcg
     2161 agggtcgcct ccgccggggg caggccctgg ctccgctcgc gggccgccgc cgcttcctga
     2221 tcgtaggccg cgctgacggc cgcgagcacg tccgcggcgg tgcgcccggc gaggccgtcg
     2281 cgatcgtagt cgtcgatgta gacctgtccg tacaccgccc gggcgcggtt gtccacgaac
     2341 atgatcgagg cgcgggaccg gctcacgggc accaggaccg cggacagcct aacggtggtg
     2401 gcgagcgcgt tcgccgtcgt cagctggaac tcggcctctt gcagcagtct gggctccagc
     2461 gccagcgccg cctcgcgctc ggcccacagc gcccgtcccg gcgcgaaccc cggatgctcg
     2521 agcgtcagcc ggcggagtgc ctgcccgacg cgcgggtcgg acgcgccgaa ggcgaggtag
     2581 cgcgcgaggg cctcgtctcc catcctgcct gcggcgacct ggcgcgccgc tgcctcctcc
     2641 tgccggcgcc gcccctcggc atcgccggcg ggtttcaggt acggcaggat gctctcgcga
     2701 tgggcggcga gcgcctcgac gttgcccctc atcaccgaag ccgcagccat cgagaacggc
     2761 gccgagaact ccaggtagag ccggtcgtcc gtgttgagga caccgttccg cccgaagcgc
     2821 gtcataccct cggtgcccat caggaagtac gccagcaggt cggcggccga gcccatgttg
     2881 acgcgccgca ggtcctcggc gacgccgggc gccgcgatcc ggcggtcgag gtcagcttcg
     2941 tccaccagga acggcgagcc ccgccccacg atcaccgcgt cgtagtgggt gacccagagc
     3001 atcgtgtggg cgaagtgctg ccggacggtc ttgacgaccg acgcgagatc ctccggcctc
     3061 agttcgtaga gcggaagcca ctgggcgatc accccgcccg ggcgaaggtg ccgcgccgcc
     3121 agcgcgaagt actcgctcga ataaaggtat ccggcgcccc tgaaccacgg atggatcggg
     3181 tccgccgtga tgacatcgaa cgtccggttc gtggtcgtca ggaagttgcg gccgtcgttg
     3241 acgacgacac tgaccttcgg gtggtccagg aggttgtggt tgtaggcgga gaacgtcctg
     3301 gcgacgccgc gcacctggcc ctcgatttcc acgagcgtca cccgatcgac gccgggatgc
     3361 acggcggtcg cccccgctgt catcccgctc cccatcccga cgacgaggac gtccgagggc
     3421 cgctcgcaca agagcatcgg cagatgcccg agtgtgaact ggacctgctg cgcctgcagg
     3481 tgcgtcgagg cctcgatccg gccgttggtg acgaaggcct gttcgccgcc cttgacccgg
     3541 atgacgctga cggtggactc gacgccctcc gagtagtaga tcacgtcggt gttctcgacg
     3601 gcctcgcgca ccatctcggg cgtgcggaag gcctcgggct ggttgctcct gtagatcgcg
     3661 aagtacttgc ggtcccagat cctcgccgca tccgggctgg aggccaagaa cgccagcacg
     3721 gcggcggtgc acgccgccgc gcccgccggc agccaccgcg tgcggcggag gctggcgagc
     3781 acgagcaggc ccaggccggc gtttgcgacg gtcaacagct ggagggaccg ctcgatgccg
     3841 acgagccgga tcaggatcag cccgctgacg gcggctccga ggatcgatcc aaccgtgttg
     3901 gacgcgaaca cgtcgccgac gccccggccg accgcccgcc gctggcgcgc cacgacgtct
     3961 cccgcgagcg ggaacgcggc gcccatgaag aacgcgggga cgaccatgta caggaacgcc
     4021 aggccgaagt tggcccacgt ccgcgcctgg aaggagccgg gctcggatcc gaagacgaag
     4081 ccctgcagcc gaacggcagc gacggggatg tcgcgcaggt ataccgtgac gagcagggcg
     4141 gtcacgccga cggcgatctg ggttgcgccg aaccaggcgg ccacgcgcct ggcggccggc
     4201 acggccgccc tggaggcccg cgcgaagacc ccgtaggcct cgctgcccag cgcgatgccc
     4261 gtcaggaacg ccaccagtat gatcgtgaag ccgtagacgc tggcgccgac ggcgatggtc
     4321 agcacccgcg tccagag
//
LOCUS       DAOSIF010000250         4329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17926_length_4329_cov_2.916472, whole genome shotgun sequence.
ACCESSION   DAOSIF010000250 DAOSIF010000000
VERSION     DAOSIF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17926_length_4329_cov_2.916472"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLT35_08890"
     CDS             complement(<1..283)
                     /locus_tag="PLT35_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOG29383.1"
                     /translation="MKIAVCLKQVPARDWAPRVDDAGTWVRDQDAGFELNEPDAYALE
                     EGLRLKEQHGGEVVVCSAGPARVSQALREALARGADRAIHVEDDGLAVAD"
     gene            complement(302..1543)
                     /gene="fabF"
                     /locus_tag="PLT35_08895"
     CDS             complement(302..1543)
                     /gene="fabF"
                     /locus_tag="PLT35_08895"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF004970.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOG29384.1"
                     /translation="MNRRVVITGIGLVSPLGIGTEATWEGLCAGRSGVGRITRFDASP
                     FSVQIAAELKGFDPLQFIDKKDVRKMDPFIHYAIAASQLAVADARLEIGPENAARVGV
                     FIASGIGGFGTFEREYKAYLEGGPRKLSPFFVPAMIINLAAGQVSIRLGAKGPNLSTS
                     TACAASAHAVGEASEIVRRGAADVMIAGGSEASITAMGIGGFTAMRALSTRNDEPERA
                     SRPFDRDRDGFVVGEGAGIIVLEELESARRRGARIYAEVAGYGATGDAFHITAPSEDG
                     DGAIRAMAAALASAGVSPSEVQYINAHGTSTPYNDKLETLAIRTLFGEHANALAVSST
                     KSMTGHLLGAAGGVETGITALAVFHQVMPPTINQDTPDPECTLDYVPWTARKAPIGCA
                     LSNSFGFGGTNAALLLRRCES"
     gene            complement(1563..1805)
                     /gene="acpP"
                     /locus_tag="PLT35_08900"
     CDS             complement(1563..1805)
                     /gene="acpP"
                     /locus_tag="PLT35_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG29385.1"
                     /translation="MAGVADKVKSIIVEQLGVDEEEVTVDASFVDDLGADSLDTVELV
                     MAFEEEFGIEIPDEDAEKITRVKEAVEYIEAHAKKK"
     gene            complement(1814..2557)
                     /gene="fabG"
                     /locus_tag="PLT35_08905"
     CDS             complement(1814..2557)
                     /gene="fabG"
                     /locus_tag="PLT35_08905"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOG29386.1"
                     /translation="MFELTGKVAVVTGAARGIGRAIAWALASRGALVVPTDAVVSSPD
                     VGDSPAPGSGRIADLVRMNVTEAADVEAVFAGVLAEHGRLDILVNNAGITRDQLLMRM
                     KREEWDRVLEVNLTGAFVCSQAVLRPMMKQRSGRIISISSVVGQMGNAGQSNYAASKA
                     GLIGFSKSLAREVAARTITVNVVAPGLIDTDMTRALPEAVQAEWQSKVPLGRFGTPAD
                     VAAAVCFLASEEASYVTGQVLAVNGGMYT"
     gene            complement(2564..3490)
                     /gene="fabD"
                     /locus_tag="PLT35_08910"
     CDS             complement(2564..3490)
                     /gene="fabD"
                     /locus_tag="PLT35_08910"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOG29387.1"
                     /translation="MIAFIFPGQGSQAVGMGKALAEAYPAARETFAEADEALGERLSG
                     LCFDGPADRLVLTENTQPAILAVSVACFRVLASRGLKPDFVAGHSLGEYSAHVAAGTL
                     AFADALRTVRRRGRYMQEAVPVGQGAMAAILGLDAAKVAQACEEAAEGEVVSPANLNA
                     PGQVVIAGAKGAVARASEAAKRLGARRAITLPVSAPFHCALMKPAEVRLAPDLRALPA
                     GDPLVPVVANVDAEVKRSGRDAIDALIRQVSSPVRWEDVMRRLASEGVRKYVEVGPGT
                     VLTGLGRKTLPDAAFASAEDPAGVDAAAALLG"
     gene            complement(3651..3833)
                     /gene="rpmF"
                     /locus_tag="PLT35_08915"
     CDS             complement(3651..3833)
                     /gene="rpmF"
                     /locus_tag="PLT35_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOG29388.1"
                     /translation="MPNPKRRHSKARTAKRRTHDALKPVSLGRCPHCHEAKAPHRICP
                     HCGYYRGRQVRDVKEV"
     gene            complement(3836..>4329)
                     /locus_tag="PLT35_08920"
     CDS             complement(3836..>4329)
                     /locus_tag="PLT35_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOG29389.1"
                     /translation="PEVRIDRTFAAGVLPTDPRMFRIAAPLRLAVVVRKDRSQFRLVG
                     RVRTTVALACSRCLEDFALELDEPFDVLYLPRGHSGDAEERQVEDDDLSTAFYSDNVI
                     DLGQLMQEQCYLAVPMKPLCSDGCRGLCPECGTNLNSGSCNCQHVWVDPRMAVLEQLR
                     KDQ"
BASE COUNT          633 a         1599 c         1453 g          644 t
ORIGIN      
        1 ggtcggcgac tgccaggccg tcgtcctcga cgtggatggc gcgatccgcg cccctggcca
       61 gcgcctcgcg cagcgcctgg gacacgcgcg cggggccggc cgagcacacg acgacctcgc
      121 cgccgtgctg ctctttcagg cgcagcccct cttcgagggc gtacgcgtcc ggctcgttga
      181 gctcgaagcc cgcgtcctgg tcgcgcaccc acgtgcccgc gtcatcgacc cgtggcgccc
      241 agtcgcgcgc cggcacctgc ttgagacaca cggcgatctt catggcgaag cggtcccggc
      301 gtcagctctc gcagcggcgg aggagcaacg cggcgttggt gccgccgaac ccgaacgagt
      361 tggacagggc gcagccgatc ggcgccttgc gagccgtcca cggcacgtag tccagcgtgc
      421 actccgggtc gggcgtgtcc tggttgatcg tcgggggcat cacctggtga aagaccgcga
      481 gggccgtgat gcccgtctcc acgccgcccg ccgctccgag caggtggccg gtcatggact
      541 tggtcgagga gacggccagg gcgttggcat gctcgccaaa cagggtccgg atggccagcg
      601 tctcgagctt gtcgttgtac ggcgtcgagg tgccgtgcgc gttgatgtac tgcacctcgg
      661 agggcgagac gccggcgctc gcgagggccg cggccatcgc ccggatcgct ccgtcgccgt
      721 cctcggaagg cgcagtgatg tggaaggcgt cgccggtggc gccgtagccg gcgacctcgg
      781 cgtagatgcg cgcgcctcgc cggcgcgcgg actccagctc ctcgagcacg atgatcccgg
      841 cgccctcgcc caccacgaag ccgtcccgat cgcggtcgaa gggcctgctc gcccgctcgg
      901 gctcgtcgtt gcgggtggag agcgcccgca tggcggtgaa gccgccgatc cccatcgccg
      961 tgatcgacgc ctcggatccg ccggcgatca tcacgtcggc ggcgccgcgc cggacgatct
     1021 cggacgcctc gccgacggcg tgcgccgagg ccgcgcaggc cgtgctggtc gagaggttgg
     1081 gccccttggc cccgaggcgg atggacacct gaccggccgc caggttgatg atcatggccg
     1141 gcacgaagaa cggcgacagc ttgcgcggcc cgccctccag gtacgccttg tactcgcgct
     1201 cgaacgtgcc gaagccgccg atcccggagg caatgaacac gccgacacgg gcggcgttct
     1261 ccggcccgat ctcaagccgc gcgtccgcca cggcgagctg cgaggccgcg atcgcgtagt
     1321 ggatgaaagg atccatcttc ctgacgtcct tcttgtcgat gaactgcaag ggatcgaacc
     1381 ccttgagttc ggcggcaatc tggacgctga agggcgacgc atcgaatctc gtgatgcgcc
     1441 cgacgccgct gcgtccggcg cacaatcctt cccacgtcgc ctcggtgccg atgcccagcg
     1501 gcgacaccag cccgatgccc gtgatgacaa cgcgcctgtt cacgcggcct ccctaggggt
     1561 tgctactttt tcttggcgtg ggcttcgatg tactcgacgg cttccttgac gcgggtgatc
     1621 ttctcggcat cctcgtcagg gatctcgatg ccgaactcct cctcgaacgc catgaccagt
     1681 tcgaccgtgt cgagcgagtc ggcgccgagg tcgtcgacga acgaggcatc gacggtcact
     1741 tcctcctcgt cgacgccgag ctgctccacg atgatgctct tgaccttgtc agcaacccca
     1801 gccattcatc ctcctaggtg tacatcccgc cgttgacggc gagaacctgc cccgtgacgt
     1861 acgacgcctc ctccgacgcc aggaaacaga ccgccgctgc cacgtcggcg ggcgtcccga
     1921 accggccgag aggcaccttc gactgccact ccgcctggac cgcctccggc agcgcgcgcg
     1981 tcatgtccgt gtcgatgagg cccggcgcca ccacgttcac ggtgatggtc cgcgcagcga
     2041 cctcgcgcgc gagcgacttg ctgaacccga tgagcccggc tttcgacgcg gcgtagttcg
     2101 actggccggc attgcccatc tggccgacaa cggagctgat gctgatgatc cgcccggacc
     2161 gctgcttcat catcgggcgg agcaccgcct gcgagcacac gaacgcgccc gtcaggttga
     2221 cttccagcac gcggtcccac tcttcccgct tcatgcgcat cagcagctgg tcgcgggtga
     2281 tcccggcgtt gttgaccagg atgtcgaggc gcccgtgctc ggccagcacg ccggcgaaca
     2341 cggcttcgac gtcggcagcc tcggtgacgt tcatgcggac caggtccgcg atccgcccgg
     2401 aacccggagc gggcgaatcg ccgacatcgg gtgaggagac cacggcgtca gtgggcacga
     2461 ccagcgcccc ccgcgaggcc agggcccatg cgatggcacg cccgattccc cgggcggccc
     2521 cggtgacgac ggcgaccttg ccagtcaact cgaacatgcg gcgctacccc agcagggcgg
     2581 ccgcggcgtc caccccggcg gggtcttccg cgctcgcaaa cgccgcgtcc ggcagggttt
     2641 tccggccgag acccgtcagg accgtccctg gacccacttc aacatacttg cggacgccct
     2701 ccgatgcaag ccgccgcatc acgtcctccc agcggaccgg cgacgagacc tgccggatga
     2761 gcgcgtcgat cgcgtcgcgc ccgctccgct tcacctcggc atccacgttg gccacgacgg
     2821 gcacgagggg atcccccgcc ggcagcgctc gaagatccgg cgcgaggcgg acctcggcgg
     2881 gcttcatcag ggcgcagtgg aacggggcgc tgaccggcag cgtgatcgcc cgcctggcgc
     2941 cgagtcgttt ggccgcctca gacgccctgg ccacggcgcc cttggcgccg gcgatgacga
     3001 cctgccccgg cgcgttcagg ttggccgggc tgacgacctc gccctccgcc gcctcctcgc
     3061 aggcctgcgc taccttcgcg gcgtccaggc cgaggatcgc agccatggcg ccctgcccca
     3121 ccggcaccgc ttcctgcatg tagcgcccgc gcctacggac ggtgcgcagc gcgtcggcaa
     3181 acgccagcgt gccggccgcg acgtgcgcag agtattcgcc gagcgaatgc cccgccacga
     3241 agtccggctt cagccccctg gacgccagca cgcggaaaca cgccacgctg acggccagga
     3301 tcgccggctg cgtgttctcc gtcagcacga ggcggtccgc cgggccgtcg aaacacaagc
     3361 cgctgagacg ctcgccgagc gcctcgtcgg cctcggcgaa cgtctcccgc gcggctggat
     3421 acgcctcggc cagcgccttg cccatgccaa cggcctgcga cccctgtccg gggaagatga
     3481 aggcgatcac gcgcaccctc ccacgcaagc cccccgggca ctccggcacg gagggaaatg
     3541 ccgggcaccg acgacgcggc cgggagccgc cccgcctgcg gcgcggcccg cccggctagc
     3601 cgcgccacga gtggacgcgc gcggctccgg cgccccggcg gcaggcgacc tcagacttcc
     3661 ttgacgtccc gcacctggcg gccgcggtag tagccgcagt gcgggcagat ccggtgcggc
     3721 gccttcgcct cgtggcagtg cgggcagcgg cccagcgaca ccggtttcag cgcgtcgtgg
     3781 gtccggcgct tcgccgtgcg ggccttcgag tgtcgtcgct tcggattcgg catcgctact
     3841 ggtccttgcg cagctgctcc agcaccgcca tccgcgggtc gacccacacg tgctggcagt
     3901 tgcaggagcc cgagttcagg ttcgtgccgc actcagggca caatccccgg cagccgtcgc
     3961 tgcagagcgg cttcatcggc acggccaggt agcattgctc ctgcatcagc tggccgaggt
     4021 cgatgacgtt gtccgaatag aacgccgtcg aaaggtcgtc gtcctcgacc tggcgctcct
     4081 cggcgtcgcc ggaatggcct cgggggaggt agagcacgtc gaacggctcg tccagctcca
     4141 aggcgaagtc ctccaggcat cggctgcacg ccagggcgac tgtcgtcctg acgcgcccga
     4201 cgaggcggaa ctgggaccga tccttgcgca cgacgaccgc gagtcgaagc ggggccgcga
     4261 tccgaaacat gcggggatcc gtcggcagca ccccggcggc gaaggtccgg tcgatccgga
     4321 cctccgggt
//
LOCUS       DAOSIF010000251         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17959_length_4323_cov_3.417526, whole genome shotgun sequence.
ACCESSION   DAOSIF010000251 DAOSIF010000000
VERSION     DAOSIF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17959_length_4323_cov_3.417526"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLT35_08925"
     CDS             <1..523
                     /locus_tag="PLT35_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG29390.1"
                     /translation="GKKFVVSVVNTGMGLPPVVVGLWVYMLLSRSGALGSFAWLFTPA
                     AMVLAQTIIAFPLVAALTMSAVEGVDPELRLQVRALGATRRQSGWTVLREARIGLTAA
                     IVAAFGGIISEVGAVMLVGGNIEGHTRVLTTAIVLSTRQGDFGLAMALGTVLLLLALG
                     ANAFLLQLQGRAR"
     gene            520..1248
                     /locus_tag="PLT35_08930"
     CDS             520..1248
                     /locus_tag="PLT35_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOG29391.1"
                     /translation="MKAGATPAYALQGIRHRYGDRTVLEIDRLEIAAGRTLGVVGPSG
                     AGKSTLLRLLQGLERPAEGEIRVDGELFPYPAPLALARRITTVFQRPAMLDRSVRDNV
                     TFGLRLRGKAEEPVVDALLERLGLAPLARADARKLSGGETQRVALARALAVEPDVLLL
                     DEPAANLDPAHVALVEALIRDVQARGTTIVLVTHNTHEARRLAHDTLLLLDGKMVEAA
                     PTAAFFESPTNARARAFLAGELVY"
     gene            complement(1334..1789)
                     /locus_tag="PLT35_08935"
     CDS             complement(1334..1789)
                     /locus_tag="PLT35_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOG29392.1"
                     /translation="MRIYLACTVRGDRGAIDAARHIHACLVGLGHEVLTGHLLRDDVA
                     AAEDEVGDAAVFARDMAWLESADAVVAEASGSSYGVGFEVGYALGRSEQTGQRVLVLY
                     DASRRGRVSRLIAGMNDPRARVLAYESPSDIDAFLASSLSTATISPSVE"
     gene            complement(2044..2604)
                     /locus_tag="PLT35_08940"
     CDS             complement(2044..2604)
                     /locus_tag="PLT35_08940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG29393.1"
                     /translation="MSTARPAMLFDLDDTLFDHSGCARDALGVLRERYPALPVPAPAV
                     MAREYQRVLEEQHLRVPAGELGIDEAHVRRFERLCARAGIPVAADTAQEAAAACRTAD
                     LAACLEACRQADGAKDRLAAFEEICATGVVTNTPATEQRRKLAVCGFDAFLDAVVIAE
                     ETGRAKPDPASSRPRWSASAGAWKRP"
     gene            complement(2610..3680)
                     /locus_tag="PLT35_08945"
     CDS             complement(2610..3680)
                     /locus_tag="PLT35_08945"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOG29394.1"
                     /translation="MALIDLRSDTLSKPTPDMLQSMAAATLGDDSRDGDPTVRELEAE
                     AAQLLGKDAALLTVSGTMSNTVALRTHAEPGGAAVVEESAHVYGMEHGSIASACGLLA
                     MPIRGHRGAPDLDAVRWAIRRARTLFPASGLVCLENTHNAAGGAVLTPAQIDGVAEAA
                     HQGGFAVHLDGARLFNAAVALGVDARELTRSVDSVSVCLSKGLSAPIGSLLAGPKAFI
                     ERANRVRRAFGGAMRQAGILAALGLVALRTMIPRLAEDHDNARRFARAVAGVPGLSVD
                     LSAVQSNIVHVDVGGLGIEAAAFAAHLREWGVRGLPGMGSSMRFVTYRGITRADVEEA
                     ARAAAALVEARPWAARGSVERG"
     gene            3948..>4323
                     /locus_tag="PLT35_08950"
     CDS             3948..>4323
                     /locus_tag="PLT35_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29395.1"
                     /translation="MSIAASRITRGWRLGDPWSIAEASELYEIARWGQGYFSIGPSGN
                     LHVHPTKEPDKYIDLKALTENLVLRGIDIPVLIRFSDILRHRVQDLHDAFQTAITQNG
                     YQGRYVCVYPVKVNQQRRVVEEI"
BASE COUNT          625 a         1566 c         1485 g          647 t
ORIGIN      
        1 gggcaagaaa ttcgttgtca gtgtggtcaa caccggcatg gggctcccgc cggtcgttgt
       61 cggcctctgg gtctacatgc tcctctcgcg gagcggcgcg ctcggctcgt tcgcgtggct
      121 cttcacgccg gcggccatgg tcctcgcgca gaccatcatc gcgttcccgc tcgtcgcggc
      181 gctcaccatg agcgcggtgg agggcgtcga tcccgaactg cggctgcagg tgcgcgccct
      241 cggagccact cgacggcagt cgggctggac cgtgctgcgg gaggcgcgca tcggcttgac
      301 ggccgccatt gtggcggcgt ttggcggcat catttcggaa gtcggcgcgg tgatgctagt
      361 cggcggcaac atcgagggcc acacgcgcgt acttacgacg gccatcgtgc tgtcgacgcg
      421 ccagggcgac ttcggcctcg cgatggcgct ggggaccgtg ctcctgctgc tcgcgctcgg
      481 ggcgaacgcc ttccttctgc agctccaggg gagagcgcga tgaaggccgg cgcaacaccg
      541 gcctacgccc tgcaaggcat ccgtcaccgc tacggcgacc ggacggtgct ggagattgac
      601 cgcctcgaga ttgccgcggg ccggacgctc ggcgttgtcg ggcccagtgg cgccggcaag
      661 agcaccctcc tgcgcctgct gcagggcctc gagcggccgg ccgagggcga gatccgcgtc
      721 gatggcgagc tttttcccta tcccgcgccg ctcgcgctcg cacgccggat cacgacggtc
      781 ttccagcggc cggcgatgct cgatcgcagc gtgcgcgaca atgtgacgtt cggtctccgc
      841 ttgcgcggga aggccgagga acccgtggtg gatgccttgc tcgagcggct cggcctggct
      901 cctctggccc gtgccgatgc ccggaagctc tcaggcggcg agacccagcg cgtggcgctc
      961 gctagagcgc tggccgtcga accggacgtc ctgctgctcg acgaacccgc cgccaatctc
     1021 gaccctgcgc atgtcgccct ggtcgaggcg ctcattcgcg acgtccaggc ccgcggcacc
     1081 accatcgtcc tggtcacgca caacacgcac gaggcccgcc ggctggccca cgacacgctg
     1141 ctgctgctcg acgggaagat ggtcgaggcg gcgccgaccg ctgcgttctt cgagtcgccg
     1201 accaacgccc gcgcccgggc ctttctggcg ggcgaactcg tctactaggc gcacggcgcc
     1261 gcgcctctcc agctcctcgg gcaggggcgg cagccgtaca tggtcagatg gccgagcggc
     1321 ctgccccccg tggtcattcg accgaagggg agatggtcgc cgtcgacaga ctggaagcca
     1381 ggaaggcgtc gatgtccgac ggggactcgt aggccagcac gcgcgcacgg gggtcgttca
     1441 ttccggcaat caaccgcgat acccggccgc ggcgcgaggc gtcgtacagc accaacacac
     1501 gctgtccggt ctgttcggac ctccccagcg cgtagcccac ttcgaacccg acgccgtagc
     1561 tcgacccgga ggcttctgcg acgacagcat ccgccgactc aagccaggcc atgtcgcgcg
     1621 cgaacacggc ggcgtcgcca acctcgtcct cggcggcggc gacgtcgtct cgcagcaggt
     1681 gcccggtgag cacctcgtgg ccgagaccga cgagacaggc gtgaatgtgg cgggcggcat
     1741 cgatggcgcc acggtcgccg cggacggtgc aggctaggta gatacgcatc gctcagactc
     1801 gggactcggg gtttggaacc cgggattcgg ggttcggggg tttggaactc gggcctcggg
     1861 actccggagg ctgcccctgc aattcctcga cgacgacgga caccacgagt tccgtcgggc
     1921 ggcgcgaccg aatctcggcg accgtctcat cagacgaggg cgcgcccgag ggattgaccc
     1981 agacaggccg ggcacaagcg gcgtgcgcgt ctcggatgtc cgcgtctcgg gcgttcccga
     2041 tcatcacggc ctcttccatg caccagccga ggcgctccag cgcggtctcg aagatgcggg
     2101 gtccggcttc gcgcggcccg tctcttcggc gatcaccacg gcgtcgagga acgcgtcgaa
     2161 gccgcagacg gccagcttcc ggcgctgctc cgtggctggg gtgttggtga cgacgccggt
     2221 agcgcagatc tcctcgaagg ccgccagccg atccttcgcc ccgtccgcct gccggcacgc
     2281 ctcgaggcag gctgcgaggt cggccgtcct gcaggcggca gcggcctctt gcgccgtgtc
     2341 ggcggcgacg gggatcccgg ctcgggcaca cagccgctcg aaacgccgga catgcgcttc
     2401 gtcgatgccg agctcaccgg ccgggacacg gaggtgctgt tcctccagca cgcgttggta
     2461 ctcccgcgcc atgacagcag gcgccgggac cgggagggcg ggatagcgct cgcgaaggac
     2521 gccaagcgca tcgcgcgcgc agccgctgtg gtcgaagagc gtgtcgtcga gatcgaaaag
     2581 catcgccggc ctggccgtgg acatggccgt catcctctct cgacgctccc gcgagccgcc
     2641 cacggcctcg cctcgacaag ggcggccgcg gcgcgggccg cctcctcgac atcggcccgg
     2701 gtgatgccgc ggtacgtgac gaaccgcatg ctggagccca tccccgggag tccccggacg
     2761 ccccactcgc gcaggtgcgc cgcaaacgcc gccgcctcga tgccgaggcc gccgacgtcc
     2821 acatggacga tgttgctctg gacagcggag agatccaccg agagcccggg aacgccggcc
     2881 accgcccgag cgaacctccg ggcgttgtcg tgatcctccg cgaggcgcgg gatcatcgtg
     2941 cggagcgcca cgagtcctag cgcggcgagg atgcccgcct gccgcatcgc gccgccgaag
     3001 gcccgccgca cgcggttcgc gcgctcgatg aacgccttcg gcccggccag cagggatcca
     3061 atcggcgccg ataacccctt cgacaggcag accgagaccg aatccaccga ccgcgtcagc
     3121 tcgcgcgcgt ccacgccgag cgcaaccgcc gcgttgaaga ggcgcgcgcc gtcgaggtga
     3181 acggcgaagc cgccctggtg tgcggcctcg gccacaccgt cgatctgcgc aggcgtcagg
     3241 accgcgccgc cggcggcgtt gtgggtgttc tcgaggcata cgaggcctga ggccgggaac
     3301 aacgtcctgg ccctccggat cgcccaccga acggcatcga ggtccggggc gccccgatgt
     3361 ccccggatcg gcatggcgag cagcccacac gccgacgcga tcgagccgtg ctccatcccg
     3421 tagacgtggg cggactcctc gacgacggca gcgcctcccg gttcggcgtg ggtccgcagt
     3481 gcgacggtgt tggacatcgt cccgctcacg gtgagcagcg ccgcgtcttt gccgagcagc
     3541 tgcgccgcct cggcttcgag ttcgcgcacc gtcggatcgc cgtcacggga gtcgtctccg
     3601 agcgtcgcgg ccgccatgct ctgcagcatg tcgggcgtcg gcttcgacaa cgtgtcgctg
     3661 cgcaaatcga tcagcgccat cgtccgcctt tcgcctcgtg ccgtgacccg gcggcacggc
     3721 ctttccattt cgctggggtt cctgggcctt cgggccgcgc ccgccgggcc tgccgcgccg
     3781 gtctccgcct ggccgcacct gcgcccgaag cgcccctcgg tctcgtggcc ccctgggccc
     3841 tcacgccgtc ttggcgtcgg cgccggcgcc gcggcccgcg gacgccattg tacgcccgcc
     3901 ccgaaaacct ccgcggcgcc cggctgaact tctgtattat ggaacgcgtg agcattgcag
     3961 ccagccgcat cacccggggg tggcgcctcg gcgacccgtg gagcatcgcc gaggcgtccg
     4021 agttgtacga gatcgcccgc tggggccagg gctacttctc gatcgggccg agcggcaacc
     4081 tgcacgtgca cccgacgaag gagccggaca agtacatcga cttgaaggcg ctgaccgaga
     4141 atctcgtgct gcgcggcatc gacatcccgg tgctgatccg gttcagcgac atcctccggc
     4201 accgcgtcca ggatctgcac gacgccttcc agacggcgat cacgcagaac ggctaccagg
     4261 gccgctacgt ctgcgtctac cccgtcaagg tcaaccagca gcgccgcgtc gtcgaggaga
     4321 tcg
//
LOCUS       DAOSIF010000252         4318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_17988_length_4318_cov_2.936899, whole genome shotgun sequence.
ACCESSION   DAOSIF010000252 DAOSIF010000000
VERSION     DAOSIF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17988_length_4318_cov_2.936899"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLT35_08955"
     CDS             complement(<1..475)
                     /locus_tag="PLT35_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG29396.1"
                     /translation="MVDRVFTRAARRCKLGCQQLGAAMRTTETSARFGNKLLAEREKA
                     GVSTTHLAKEVGVSLSTLSRIEAGSLSPSRDLAERIAGAFEQSVEGWFLAAGIVPERV
                     AALLAESPDTFEAVSRIAPPAVVARARLEGLIADIRNRHPALARMDGLRASSLCLL"
     gene            626..1018
                     /locus_tag="PLT35_08960"
     CDS             626..1018
                     /locus_tag="PLT35_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29397.1"
                     /translation="MKRVKTFRFSEADLAVLDELSEAFGGASEAETLRRLLAEGLRYR
                     MEHCTPPVHPHFLDAIKPEAMPQEFLEYLDAVREQVGVMRPDGTAPTHYFAQVPAGGR
                     WEAEGVAGGWAVLDKRTKFTRNGYEREV"
     gene            1021..2889
                     /locus_tag="PLT35_08965"
     CDS             1021..2889
                     /locus_tag="PLT35_08965"
                     /inference="COORDINATES: protein motif:HMM:NF021628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HOG29398.1"
                     /translation="MTTQQADIAAAPSELALLASIDGRKLPRVEIVAYTGGVMSVPGW
                     GDVAIDLSGLELSATVPILSDHDSTRRGIVGHGRAEAKAGRLLLAGSISAGTAAAREI
                     VEAAKNGFPWQASVAAEVLERRQVPAGQSAAVNGKTLAAPAGGMTLIVRGRLREVSIV
                     GIGCDSATSVAIAASRTNTEQKNMEATNIERGEQAMEAARLEAAELARVESIRRICAG
                     RHDRIEAQAVADGWDANRTELEVLRADRPKAPAVHLPQRAAGTVETIEAAVLTWMGQA
                     GLAEKTLGPAAMEQAGRLGATCLLDLCRSALLADGKEPPRGRMEMVKAALSTYSLPTA
                     LGNVANKVLLDAYNEVPASWRAFASIRSVPDFKTNSAIRPSFTGALEQVAHGGELKHG
                     TVGESVAEFAIDTFGKLLSIDRRDIINDDLGLFQDAARAMGRSALRKLSDLVYAVLLA
                     NAGSFFGTGNKNLLTGADSALCFDALAAAIALMRTQRDAEGNDLDLKPATLLVGPDLE
                     PTARALLESEYIQRPEDIPTGNSLRRAVNLEVEPRLANTAKYGTAASTKHWYLFAAPS
                     FAPMVVAFLNGLQTPTVEYFGLEQSVERLAVTWRVYHDFGASLVDPRAAVRSKGEA"
     gene            3039..3221
                     /locus_tag="PLT35_08970"
     CDS             3039..3221
                     /locus_tag="PLT35_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29399.1"
                     /translation="MPVAKDTSQEVVEQVAGYEIRIVRAKDGRDSRQRVEERGEAMAA
                     WLLSRWREQHRQEGRN"
     gene            3221..>4318
                     /locus_tag="PLT35_08975"
     CDS             3221..>4318
                     /locus_tag="PLT35_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG29400.1"
                     /translation="MPSQVKIANDRLRIADCMHALRGIEEGDNFDGAFRLLKACQVCI
                     EHDLSDANAIDQIRSYAMLHPFPRQYGEAEVVKYLRMAERRVRRGGAYMPKAAPSGPA
                     CEPGQLSPQYRSVEQLVMQFPHLRKPLIEGLLREGETMNVIAPPKTGKSWLVTDLAIA
                     VATGRPWLDTFQTHRGSVLIIDNELHGETSAHRIPKVAEARGVPLEEIDQMVYVENLR
                     GRLRDLMEMSVYFKAIGPRQFKLIVLDAFYRFIPRDTDENDNGSVARLYNLLDHFALE
                     LQCSFVLIHHSTKGNQSAKAITDVGAGAGSQSRATDTHLILRAHEEPEAVVLDAAVRS
                     WPKVTPLCLKWSFPVWNPAFDLDPEALKPERP"
BASE COUNT          748 a         1469 c         1502 g          599 t
ORIGIN      
        1 tcagaaggca caggctcgac gccctgagac catccatacg cgcaagcgcg ggatggcgat
       61 tgcgaatgtc ggcaatcagc ccctccaacc ttgctctggc gacgacggcc ggcggagcga
      121 ttcgtgagac cgcctcgaag gtgtcggggc tctcggctag gagcgccgcg accctctccg
      181 gcacgatgcc ggcagcaagg aaccagcctt cgaccgattg ctcgaacgcg ccggcgatcc
      241 tctcggccag atcgcggctt ggcgacaggg agccggcctc gatgcgcgaa agcgtggaca
      301 gcgatacccc aacttccttg gcaaggtgag tagtggagac gccggccttc tcgcgttctg
      361 cgagaagctt gttcccgaac cttgcgctgg tctcagtcgt ccgcatcgcc gctcccaatt
      421 gctggcagcc aagcttacat cgccttgcag cccttgtaaa gactctgtcg accatggcga
      481 gatggatttc ctcgccctcc gctcagccac ttccggccgg ccccggtaag caccagacag
      541 gcgacgcgga cggattcgcg cgaccacctg aagcgctttc cggtaagccc tgaacatgga
      601 gcacgagaac acgaaggagt gtgccatgaa gcgagtcaag acgttccggt tttctgaggc
      661 cgacctggcg gtgctggacg aactgagcga ggcgttcggg ggcgcgtccg aggccgagac
      721 gctccggcgg ctgctcgccg aggggctgcg gtatcggatg gagcactgca cgcccccggt
      781 gcatccgcat ttcctcgacg ccatcaagcc ggaggccatg ccccaggagt tcctggagta
      841 cctcgacgcc gtgcgcgagc aggtcggcgt catgcggccc gacggcacgg ccccgacgca
      901 ctacttcgcg caggttcccg caggcggacg gtgggaggcc gaaggcgtgg ccggcggatg
      961 ggcggtcctc gacaagcgga ccaagttcac gcgcaacggc tacgagcggg aggtgtgacc
     1021 atgaccacgc agcaagcgga tatcgccgcg gccccgtcgg agcttgccct gctggccagc
     1081 atcgacggcc gcaagctccc gcgcgtcgag atcgtcgcct acaccggcgg cgtgatgagc
     1141 gtgcccggct ggggcgacgt ggccatcgac ctgtcgggcc tggagctatc ggccacggtc
     1201 ccgatcctct cggaccacga cagcacgcgc cgaggcatcg tcggccacgg ccgggcggaa
     1261 gcgaaggccg gccggctgct gctggccggg tcgatctcgg ccggcacggc tgccgcgcgg
     1321 gagatcgtcg aggcggcgaa gaacggattc ccctggcagg catcggtcgc ggccgaggtg
     1381 ctggagcgcc ggcaggttcc cgccggccag tcggccgcgg tcaacggcaa gaccctcgcc
     1441 gcgccggccg gcggcatgac gctgatcgtc cggggccggc tgagggaagt gagcatcgtc
     1501 ggcatcggct gcgactcggc gacgagcgtg gccatcgccg catcaaggac gaacacggag
     1561 cagaagaaca tggaagcaac gaacatcgaa cgaggcgagc aggccatgga agccgctcgc
     1621 ctggaagcgg cggaactggc cagggtcgag tcgatccggc gcatctgcgc cggccggcac
     1681 gaccggatcg aggcgcaggc cgtcgcggac ggctgggacg ccaaccgcac ggagcttgag
     1741 gtgctgcgcg ccgacaggcc caaggccccg gccgtccacc tgccccagcg ggcagcgggc
     1801 acggtcgaga ccatcgaggc cgcggtgctc acgtggatgg ggcaggccgg gcttgccgag
     1861 aagacgctcg ggccggcggc gatggagcag gccggccggc tgggcgcgac ctgcctgctg
     1921 gacctgtgcc gctcggcgct gctggccgac ggcaaggagc cgccccgcgg ccggatggag
     1981 atggtcaagg cggcgctgtc cacctacagc ctgccgacgg cgctgggcaa cgtggcgaac
     2041 aaggtgctgc tggacgccta caacgaggtg cccgcttcct ggcgggcctt cgcctcgatc
     2101 cgaagcgtcc ccgacttcaa gaccaactcg gccatccgcc ccagcttcac gggcgcgctg
     2161 gagcaggttg cccacggcgg cgaactcaag cacggcacgg tcggcgagag cgtggccgag
     2221 ttcgccatcg acaccttcgg caagctgctc tccatcgacc ggcgggacat catcaacgac
     2281 gacctgggcc tgttccagga cgccgcgcgg gcgatgggcc gctcggccct gcggaagctc
     2341 tccgatctgg tgtacgcggt tctgctggcc aacgccggga gtttcttcgg cacgggcaac
     2401 aagaacctgc tgaccggggc cgacagcgcc ctgtgcttcg acgcgctggc cgcggcgatc
     2461 gcgctgatgc ggacccagcg ggatgccgag ggcaacgacc tcgacctcaa gccggcgacg
     2521 ctgctggtgg ggccggacct ggagccaacg gcccgggcgc tgctggaatc ggagtacatc
     2581 cagcggcccg aagacatccc gaccggtaac agcctgcgcc gagcggtgaa cctcgaagtc
     2641 gagccgcggc ttgccaacac ggccaagtac ggcacggccg cgagcaccaa gcactggtat
     2701 ctattcgccg cgccgtcgtt cgccccgatg gtcgtggcgt tcctcaacgg actccagacg
     2761 ccgacggtcg agtacttcgg cctggagcag agcgtcgagc ggctggcggt gacgtggcgg
     2821 gtctatcacg acttcggcgc gtcgctggtc gatccccgcg cggccgtgcg gagcaagggc
     2881 gaagcctgat gcgaggtcta cgggaggggc ggggttcacc agtgctcctt gccccgcccc
     2941 tccactcgtt tgagcggaac ccgcgcggcc gtcgaggcag gcccgacccg gagaccaccc
     3001 cgggcctgcc tcccggccgg cggacttctg gagtgaatat gccagttgcg aaagacacat
     3061 cccaagaggt ggtcgagcag gtcgccggat acgagattcg gatcgtccgc gccaaggacg
     3121 gccgcgactc ccgccagcga gtcgaggagc gcggcgaggc gatggctgcg tggctgctga
     3181 gccgctggcg cgagcagcac cggcaggaag gtaggaactg atgccatccc aggtcaagat
     3241 cgcaaacgac cggctccgca tcgccgactg tatgcacgcc ctgcgcggca tcgaggaagg
     3301 cgacaacttc gacggggcct tccggctgct caaggcgtgc caggtgtgca tcgagcacga
     3361 cctgagcgac gccaacgcca tcgaccagat cagaagctac gcgatgctcc acccgttccc
     3421 gcgccagtac ggcgaggccg aggtggtcaa gtacctgcgg atggccgaga ggcgggtcag
     3481 gcgcggcggc gcgtacatgc ccaaggccgc gccatcgggg ccggcctgcg agccggggca
     3541 actgtcgccc cagtaccgga gcgtggagca gcttgtgatg cagttcccgc acctgcgcaa
     3601 gccgctgatc gaagggctgc tccgcgaagg ggagacgatg aacgtcatcg cgcccccgaa
     3661 gacgggcaag agctggctgg tgacggacct ggccatcgcg gtcgccaccg gccggccgtg
     3721 gctggacacc ttccagacgc accggggaag cgtgctcatc atcgacaacg agcttcacgg
     3781 cgagaccagc gcccaccgca tcccgaaggt ggccgaggcc cgcggcgtgc cgctggaaga
     3841 gatcgaccag atggtctacg tcgagaacct ccgcggccgg ctgcgcgacc tgatggagat
     3901 gagcgtatac ttcaaggcca tcggcccgcg gcagttcaag ctgatcgtgc tcgatgcttt
     3961 ctaccgcttc atcccgcggg acaccgacga gaacgacaac ggctccgtgg cccggctcta
     4021 caacctgctg gaccacttcg ccctggagct tcaatgctcc ttcgtgctga tccaccactc
     4081 gaccaagggc aaccagtcgg ccaaggcgat taccgacgtg ggggccgggg caggcagcca
     4141 gagccgggcg accgatacgc acctgatcct tcgcgcccac gaggagccgg aggccgtggt
     4201 gctcgacgcc gcggtgcggt cctggccgaa ggtgacgccc ctgtgcctga agtggagctt
     4261 ccccgtctgg aatcccgcct tcgacctcga ccccgaagcg ctgaagcccg agcgcccc
//
LOCUS       DAOSIF010000253         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18111_length_4297_cov_3.840641, whole genome shotgun sequence.
ACCESSION   DAOSIF010000253 DAOSIF010000000
VERSION     DAOSIF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18111_length_4297_cov_3.840641"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /gene="pth"
                     /locus_tag="PLT35_08980"
     CDS             <1..473
                     /gene="pth"
                     /locus_tag="PLT35_08980"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG29401.1"
                     /translation="ESGRAETLAARFGRGDAAVLVAKPLTMMNLSGSAVAGLAGFYRV
                     DPASILVVADDVNLPLGRLRIRAAGSAGGHNGLRSIIDCLGTEGFPRLRVGVGRGDPR
                     RGLADHVLARFDGDERDEVDRAVARAADAVETFLAAGIEAAMNRHNRAEDPASS"
     gene            525..968
                     /gene="rpsF"
                     /locus_tag="PLT35_08985"
     CDS             525..968
                     /gene="rpsF"
                     /locus_tag="PLT35_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOG29402.1"
                     /translation="MSQARQYELVYIVSPDATEQDVADLHAQVEANVARYGGGMVKSE
                     NWGRRKLAYRIGKHREGTYVLEVFTGTGELTKELDRRLKVSDHVIRHLCIRVDEELRL
                     AERTKARREADQQKRIARGLSAGGAKQRAEDEGGDDDQADRAEVS"
     gene            1058..1270
                     /gene="rpsR"
                     /locus_tag="PLT35_08990"
     CDS             1058..1270
                     /gene="rpsR"
                     /locus_tag="PLT35_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG29403.1"
                     /translation="MFRRRKVCRFCADKIDYIDYKDAKMLLPFVPERAKILPRRISGV
                     CAMHQRKLQTAIMRARLLALLPYSTD"
     gene            1281..1742
                     /gene="rplI"
                     /locus_tag="PLT35_08995"
     CDS             1281..1742
                     /gene="rplI"
                     /locus_tag="PLT35_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG29404.1"
                     /translation="MEVILRDHVENLGRRGDVVKVADGYARNYLLPRKLALPATPGNL
                     KIVERQRKIADAREAAEKSGAEAIAVRLTRAELVLARRVGEHDALYGSVTAADIAGEL
                     EAQGFEIDKRKIQLAEPIKQLGEHSVPVKLHREVVAHVAVKVTRLEEAAEE"
     gene            1802..3142
                     /gene="dnaB"
                     /locus_tag="PLT35_09000"
     CDS             1802..3142
                     /gene="dnaB"
                     /locus_tag="PLT35_09000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOG29405.1"
                     /translation="MAEPAVDRPLPHNLEAERAVLGAALLNAEAVHRAVEFITERDFF
                     RDAHRRIFASMLGLAERGQAIDFITVKDELARAGELDQVGGPAYIASLVDGLPHGVNV
                     ADYARIVKEKASLRSLIASANRILASAYQAEEDAGGIIDEAEREIFAIAEGSIHGGFV
                     SMRDLANASFAVVEQAHARKQLVTGVPTGFIELDELLAGLQPSDLIIVAARPAMGKTS
                     LVLNIAQHVGTKTPMTVGVFSLEMSKEQLFLRMLTSEAGIDGHRLRTGFLGERDFPRL
                     TQAMETLGRARIYIDDSASIGVLEMRAKARRLKAEHGLDLIIIDYIQLMQGRGRFDNR
                     VTELASISRGLKGLAKELSVPVVVLSQLSRAPENRPNKRPQLSDLRESGALEQDADVV
                     VMIYREDMYEQTEENQGVAELIIAKQRNGPTGTVRLAFIREFTRFENLERGAPR"
     gene            3139..4275
                     /gene="alr"
                     /locus_tag="PLT35_09005"
     CDS             3139..4275
                     /gene="alr"
                     /locus_tag="PLT35_09005"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG29406.1"
                     /translation="MNRPTFARVDLGAIAHNVRAVRAFMAARAAGPVPALAGVVKAEA
                     YGHGAAESARAMADAGAAMVACAAIEEGVALRASGLDGPILVFGPFALGDLDGIFEYG
                     LTPTLSTPTLGRRLQDAAARRGVRLRCHLAIDTGMNRLGLRHDNLARTAPELLAAPNL
                     EFTGVCTHFATADEPGSGLFARQQRNFEEAGRVLAGLGARGCVRHAANSGALVAEPAT
                     WLDMVRPGLLLYGVPPAGFEGVVPVRPAMSVQSRLAAVKGVRPGESVGYGGRFVARRP
                     TTIGLVPAGYADGLDRRLEGRGAVLVRGRRAPIVGAVSMDVFAIDVTGLDAEPGDEVV
                     ITGRQGGDEITVSEMAAWASTIPYELLCRVGSRVERVYVRARAE"
BASE COUNT          639 a         1550 c         1558 g          550 t
ORIGIN      
        1 tcgagagcgg gagggcggag accctggcgg cgcgcttcgg ccgcggcgac gcggcggtgc
       61 tggtcgccaa gccgctcacg atgatgaacc tcagcgggtc ggccgtggcc ggcctcgccg
      121 ggttctacag ggtcgatccg gcgtcgatcc tcgtcgtggc cgacgacgtg aacctgcccc
      181 tggggcggct tcggatccgg gcggccgggt cggccggcgg gcacaacggg ctgcggtcga
      241 tcatcgactg cctcggcacc gaggggttcc cgcggctccg cgtcggcgtg ggccgcggcg
      301 acccgcggcg cggcctggcc gatcacgtgc tggcgcgctt cgacggcgac gagcgggacg
      361 aggtggatcg agcggtggcc cgggccgccg acgcggtgga gacgttcctc gcggcgggca
      421 tcgaggcggc catgaaccgg cacaaccgag cggaggaccc ggcgtcctct tgactccttg
      481 ccgccgcgga tgcgcggcgg ccgtcatgtc cagaaggagc aggcatgagc caagcgaggc
      541 agtacgaact cgtctacatc gtgtcgcccg acgcgaccga gcaggacgtc gccgacttgc
      601 acgcgcaggt cgaggcgaac gtcgcccggt acggcggcgg gatggtcaag tccgagaact
      661 gggggcgccg caagctggcg taccggatcg gcaagcaccg cgagggcacc tacgtcctcg
      721 aggtcttcac cggcacgggc gagctcacca aggagctgga ccgccgcctg aaggtgagcg
      781 accacgtcat ccgtcacctg tgcatccgcg tcgacgagga gctgcgcctg gccgagcgaa
      841 cgaaggcgcg ccgcgaggcc gaccagcaga agcgcatcgc gcgcggcctg tccgccgggg
      901 gggcgaagca gcgggcggag gacgagggcg gggacgacga tcaggctgac cgggcggagg
      961 tgtcgtgatg gctgacgaac gacagggacg gggaccgtcg ggaccgaggg ggcgcggcag
     1021 cgccaggggc ggcgagaagg gcggcccgcg ccgcggcatg ttccgccgcc ggaaggtctg
     1081 caggttctgc gcggacaaga tcgactacat cgactacaag gacgccaaga tgctgctgcc
     1141 cttcgtgccg gagcgcgcga agatcctgcc gcggcggatc tcgggcgtgt gcgccatgca
     1201 ccagcggaag ctgcagaccg ccatcatgcg cgcccgtctg ctggcgctgc tcccgtactc
     1261 gacggactag gtggaccgcc atggaagtca tcctgcgtga tcacgtggag aatctgggcc
     1321 gccgcggcga cgtggtgaag gtggccgacg gctacgcgcg caactacctg ctgccgcgca
     1381 agctcgcgct gccggcgacc ccgggcaacc tcaagatcgt cgagcgccag cgcaagatcg
     1441 ccgacgcccg cgaggccgcc gagaagtccg gcgccgaggc catcgccgtc cgcctgaccc
     1501 gggccgagct cgtcctggcc cgccgcgtgg gcgagcacga cgccctgtac gggtcggtca
     1561 cggccgccga cattgccggg gagctcgagg cgcagggctt cgagatcgac aagcgcaaga
     1621 tccagctcgc cgagccgatc aagcagctcg gcgagcactc tgtcccggtc aagctccacc
     1681 gggaagtggt ggcgcacgtg gccgtcaagg tgaccaggct cgaggaggcc gcggaagagt
     1741 agcccggggg agacagagga attcaggctt ctgagttcct gcttctgaca agatggcgcc
     1801 tatggccgag cccgccgtcg atcgcccgct tccgcacaac ctcgaggccg agcgcgcggt
     1861 gctcggcgcg gcgctgctga acgcggaggc cgtccaccgg gccgtcgagt tcatcaccga
     1921 gcgggacttc ttccgcgacg cgcaccgccg gatcttcgcg tcgatgctcg gcctggcgga
     1981 gcgcggccag gccatcgact tcatcaccgt caaggacgag ctcgcccgcg ccggcgagct
     2041 cgaccaggtc ggcggcccgg cctacatcgc gtccctcgtg gacggcctgc cccacggcgt
     2101 caacgtcgcc gactacgccc ggatcgtcaa ggagaaggcc tccctccgga gcctcatcgc
     2161 gtcggccaac cgcatcctcg cgagcgcgta ccaggccgag gaggacgcgg gcggcatcat
     2221 cgacgaggcc gagcgcgaga tcttcgccat cgccgagggc agcatccacg gcggcttcgt
     2281 gtcgatgcgc gacctggcga acgccagctt cgccgtcgtc gagcaggccc acgcccggaa
     2341 gcagctggtc acgggcgtgc cgacgggctt catcgagctg gacgagctgc tcgcgggact
     2401 ccagccctcg gacctgatca tcgtcgccgc gcgcccggcc atgggcaaga cgagcctcgt
     2461 gctcaacatc gcgcagcacg tcggcacgaa gacgccgatg accgtcggcg tcttcagcct
     2521 ggagatgtcg aaggagcagc tcttcctccg catgctcacc tccgaggctg gcatcgacgg
     2581 gcaccggctc cgcacggggt tcctcggcga gcgcgacttc ccgcggctca cccaggcgat
     2641 ggagaccctc ggccgggcgc gcatctacat cgacgactcg gcgtccatcg gcgtgctcga
     2701 gatgcgcgcg aaggcgaggc gcctcaaggc ggagcacggc ctcgacctca tcatcatcga
     2761 ctacatccag ctcatgcagg ggcgcggccg gttcgacaac cgcgtcaccg agctggcgtc
     2821 gatctcccgg gggctgaagg ggctcgcgaa ggagctctcg gtgcccgtcg tcgtgctgtc
     2881 gcagctcagc cgcgcgcccg agaaccggcc caacaagcgg ccgcagctct cggacctgcg
     2941 cgagtccggc gccctcgagc aggacgccga cgtcgtcgtc atgatctacc gcgaggacat
     3001 gtacgagcag acggaggaga accagggcgt ggccgagctc atcatcgcca agcagcgcaa
     3061 cggcccgacc ggcacggtcc gcctggcgtt catccgggag ttcacgcgct tcgagaacct
     3121 ggagcgaggc gcgccccgtt gaaccggccg accttcgccc gcgtcgacct cggcgcaatc
     3181 gcccacaacg tcagggccgt tcgcgcgttc atggcggcgc gcgccgccgg ccccgtcccg
     3241 gccctggccg gcgtcgtcaa ggccgaggcg tacggccacg gcgcggccga atcggcccgg
     3301 gccatggcgg acgccggcgc cgccatggtg gcgtgcgccg ccatcgagga aggcgtcgcg
     3361 ctgcgggcgt ccggcctcga cggccccatc ctcgtcttcg gccccttcgc cctcggcgat
     3421 ctcgacggca tcttcgagta cggcctgacg cccacgctgt ccacgcccac cctcgggcgc
     3481 cgcctccagg acgccgcggc gcgccgcggg gtgcgcctcc ggtgccacct cgccatcgac
     3541 accgggatga accgcctcgg gctgcgccac gacaacctcg cgcggaccgc gccggagctg
     3601 ctcgcggcgc cgaacctcga gttcacgggc gtctgcacgc acttcgcgac cgccgacgag
     3661 ccgggaagcg ggctgttcgc gcgccagcag cggaacttcg aggaggcagg ccgcgtgctc
     3721 gcgggcctcg gcgcgcgcgg gtgcgtccgg cacgccgcca acagcggcgc gctcgtcgcc
     3781 gagcccgcca cgtggctcga catggtccgc ccggggctgc tgctctacgg cgtcccgccg
     3841 gccggcttcg agggcgtcgt gccggttcgg ccggccatga gcgtgcagag ccgcctcgcg
     3901 gccgtcaagg gcgtccgccc cggcgagagc gtcggatacg gcggccgatt cgtcgcccgc
     3961 cgcccgacga cgatcggcct cgtgcccgcc ggctacgcgg acggcctgga ccgccgcctg
     4021 gaaggccgcg gagccgtgct cgtccggggc cgccgcgcgc ccatcgtcgg ggccgtgtcg
     4081 atggacgtct tcgccatcga cgtcaccggc ctggacgccg agccgggcga cgaagtggtc
     4141 atcaccgggc gccagggcgg cgacgaaatc accgtgtcgg agatggccgc ctgggcctcg
     4201 accatcccgt acgagctgct gtgccgcgtc ggctcgcgcg tcgaacgggt gtacgtccgg
     4261 gcgcgcgcag agtaggggcc cggcgccacc gcgccta
//
LOCUS       DAOSIF010000254         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18204_length_4280_cov_3.537041, whole genome shotgun sequence.
ACCESSION   DAOSIF010000254 DAOSIF010000000
VERSION     DAOSIF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18204_length_4280_cov_3.537041"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..519)
                     /locus_tag="PLT35_09010"
     CDS             complement(97..519)
                     /locus_tag="PLT35_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG29407.1"
                     /translation="MTPDATPFHILLVDDDEDFLFQHRIQLENAGFRVTTASTRAEAE
                     ALADQARPDLAILDLMMEQHDDGFILSHHLKRKLPALPVILVTAVTSETGISFTPTTG
                     AERAWVGADAFLSKPIRFEQLKREVDRLLEGKTPGGRG"
     gene            complement(541..2640)
                     /locus_tag="PLT35_09015"
     CDS             complement(541..2640)
                     /locus_tag="PLT35_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOG29408.1"
                     /translation="MAEGVGRRSGEGRTAARSQVALVSTIGERCRVCYQCVRECPAKA
                     IRIADHKAQVLPERCIGCGVCYQVCTQGAKRILSAVEAVDELLAGPAPVAAILAPSFP
                     AEFIRVDYPHVVGTLRAMGFASVHEVAFGAELVARQYKRLVAQDSGRLWIATTCPAVV
                     GYVRRYHPDLIGSLAPVVSPMIAIARLLRALHGDAIRVVFIGPCIAKKREAATHDLDG
                     EVDEVLTFTELRQMIAARGIDIAAVEPSGFDPPHARDGALFPISGGMLQVAGIEEDLV
                     TGHVMVGDGPAEFVQAIKEAETGIIETRLLELLFCPGCTMGPGMSSNLPHFGRRARVS
                     QFVRQRIAEVDSTAWEAEHRRWAGLSLTRSYSPYDLRMPVPSPEELQRILGDLGKPDP
                     RHELNCGACGYSSCRELASAIYVGLAEPTMCLPHSIETLRTTVRELAHSNEELASTQE
                     ALVRSEKLASMGQLAAGIAHEVNNPLGVVIMYAHFLMEQLGERPELREDLTMVVEQAD
                     RCKKIVSGLLNFARQNKVEHNLVDVGELVQKAMRSVPPPVNVVVEVENLMRNPMAELD
                     ADQITQVLVNFVTNAYAAMPDGGRLRVTARDDDGDRCSLTVADTGTGIPAEHLSRVFE
                     PFFTTKRGGKGTGLGLAVTYGIVKMHRGELAVVSNADPAKGATGTSFTVSLPRRRPEN
                     ANGHFVAATIPESAAAL"
     gene            complement(2687..>4280)
                     /locus_tag="PLT35_09020"
     CDS             complement(2687..>4280)
                     /locus_tag="PLT35_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOG29409.1"
                     /translation="ACRMCVVEIEGQRGLVPSCAFPASDGLKVQTHSPRAVDARRTLV
                     ELLLANHPDDCLYCARNGECQLQELAEQLGVRRRRYFGGRTPGRLDIASPSIARDPSK
                     CILCGKCVRVCEEVQSVGAIDFIGRGSKTRIGTAFDEGLNVSSCVNCGQCVAVCPVGA
                     LVETRAIDDVRAALADPSRIVVVQHAPSVSVSLAEEFGLKPGTDVDGPLVTALRRLGF
                     AWVFDTSFSADLTIMEEASELAHRLTTGGVLPMMTSCSAGWVKFMEQFYPDLLPNLST
                     CKSPQQMLGAIVKTFLAERLGLDPARIYSVAIMPCTAKKFEAGREEMGRAGQQDIDAV
                     LTTRELAELIRMYGLDLSALPPDAADTPFGERTSAGKLFGASGGVAEAAVRTAHYLLT
                     GQSMEAPKIQALRGLDGIKEYRTTVGGLDIGVAAVSGLGNARRLLDQVRAGRRDLHLI
                     EVMACPGGCIAGGGQPRSASTEAIRARMQALYLIDRDATVRASHENPAVKRLYAEFLE
                     KPLSHRSHELLHTHYERRQPVM"
BASE COUNT          590 a         1556 c         1493 g          641 t
ORIGIN      
        1 ctggccgtcg ccgggccgcc ggcgggccag tctcggcgcg gcgggcctgg gcctgtgtcc
       61 gttcgccgcg cccgcctggc gcctcgcccc cagcgcctag cccctgcccc ccggcgtctt
      121 cccctcgagc agccggtcca cctcgcgctt cagctgctcg aaccggatgg gcttggacag
      181 gaacgcgtcg gcgccgaccc aggcccgctc cgcccccgtg gtcggtgtga aactgatgcc
      241 ggtttccgag gtgacggccg tgaccaggat gaccggcagc gcgggcagct tgcgcttcag
      301 atggtgggac aggatgaacc cgtcgtcgtg ctgctccatc atcaggtcga ggatggccag
      361 gtcaggcctg gcctggtccg cgagcgcctc ggcctcggcc cgcgtggagg ccgtggtgac
      421 gcgaaacccg gcgttctcga gctggatccg gtgctggaac aggaagtcct cgtcgtcgtc
      481 aacgagcagg atgtggaacg gggttgcgtc gggtgtcatg gtgtggctga ccctcgggac
      541 tcagagcgcg gcggcgctct cggggatggt ggcggcgacg aaatggccgt tggcgttctc
      601 gggccgccgg cgcggcaggg acacggtgaa gctcgtgccg gtcgccccct tcgccgggtc
      661 ggcgttggag accaccgcga gctcgccgcg atgcatcttg acgatcccgt aagtgaccgc
      721 gaggccgagc ccggtgccct tgccgccccg cttggtcgtg aagaacggct cgaacaccct
      781 cgacaggtgc tccgcgggga tgcccgtgcc cgtgtcggcc accgtcaggc tgcaccgatc
      841 gccgtcgtcg tcgcgggcgg tcacgcggag ccgtccgccg tcgggcatgg cggcgtacgc
      901 gttcgtgacg aagttcacga gcacctgcgt gatctggtcg gcatcgagct ccgccatcgg
      961 gttgcgcatc aggttctcga cctcgacgac gacgttcacg ggcggcggca cgctgcgcat
     1021 cgccttctgc acgagctcgc cgacgtccac cagattgtgc tccaccttgt tctggcgcgc
     1081 gaagttgagc aggccggaga cgatcttctt gcagcggtcc gcctgctcga cgaccatcgt
     1141 caggtcctcg cgcagctcgg gccgctcccc gagctgctcc atcaggaagt gcgcgtacat
     1201 gatgaccacg ccgagggggt tgttcacctc gtgggcgatg ccggccgcca gctggcccat
     1261 gctggcgagc ttctcggagc gcacgagcgc ctcctgcgtg ctggccagct cctcgttcga
     1321 gtgggccagc tcccgcaccg tcgtccgcag cgtctcgatg ctgtgcggca ggcacatggt
     1381 cggctccgcc aggcccacgt agatggccga ggccagctcg cggcacgacg aatagccgca
     1441 ggcgccgcaa ttcagctcgt ggcgcgggtc cggcttgccg agatcgccca gaatccgctg
     1501 cagttcctcc ggggacggca ccggcatgcg gagatcgtac ggggagtagg agcgggtgag
     1561 cgacaggccg gcccaccgcc ggtgctccgc ctcccacgcg gtcgagtcca cctcggcgat
     1621 gcgctgccgc acgaactggc tgacgcgggc ccggcggccg aagtggggca ggttggagct
     1681 catgcccggc cccatcgtgc agcccgggca gaacaggagt tcgagcagcc gcgtctcgat
     1741 gatacccgtc tcggcttcct tgatggcctg cacgaactcg gccggcccgt cgccgaccat
     1801 gacgtggccc gtgacgagat cctcctcgat ccccgccacc tgcagcatgc cgccgctgat
     1861 ggggaacaag gcgccgtcgc gcgcatgggg cgggtcgaac ccgctcggct cgaccgcggc
     1921 gatgtcgatg ccgcgcgccg cgatcatctg gcgcagttcg gtgaaggtca ggacctcgtc
     1981 cacctcgccg tcgaggtcgt gcgtggccgc ctcgcgcttc ttggcgatgc aggggccgat
     2041 gaagaccacg cgaatcgcgt cgccgtgcag cgcgcgcagc aggcgcgcga tggcgatcat
     2101 tggcgaaacg accggcgcga gcgacccgat gagatccggg tggtaccggc gcacgtagcc
     2161 gaccaccgcc gggcacgtcg tcgcaatcca gagcctgccg gaatcctgcg ccaccagccg
     2221 cttgtattgc cgggccacga gctccgcgcc gaacgccact tcgtggaccg acgcgaagcc
     2281 catcgcgcgc agcgtcccca cgacgtgcgg gtagtcgacg cggatgaatt ccgcggggaa
     2341 gctgggggcg aggatggccg ccaccggcgc cggcccggcc agcagctcgt ccaccgcctc
     2401 gaccgccgag aggatgcgct tggcgccctg cgtgcacacc tggtagcaga cgccgcagcc
     2461 gatgcaccgc tcgggcagca cctgcgcctt gtggtccgcg atgcggatcg ccttggcggg
     2521 gcactcgcgc acgcactggt agcagacgcg gcagcgctcg ccgatcgtgg agaccagcgc
     2581 gacctgcgag cgagccgccg tgcgcccctc cccgctcctc cgtccgactc cttcagccac
     2641 cgttccgctc ccccttcgcg gccgcctccg gaccgcgggc cggcacctac atcaccggct
     2701 gccggcgctc gtagtgcgtg tgcaagagct cgtggctccg gtgcgagagc ggcttctcga
     2761 ggaactcggc gtacagccgc ttgaccgcgg ggttctcgtg cgacgcgcga accgtcgcgt
     2821 cgcggtcgat gaggtacagc gcctgcatcc gggcccggat ggcctcggtc gaggcggacc
     2881 gcggctggcc gccgccggcg atgcagccgc ccggacacgc catgacctcg atcaggtgca
     2941 gatcgcgccg gccggcgcgc acctggtcca gcagcctgcg cgcgttgccg aggcccgaga
     3001 cggccgcgac gccgatgtcc aggccgccca cggtcgtccg gtactccttg atgccgtcca
     3061 gtccgcgcag ggcctggatc ttgggcgcct ccatcgactg gcccgtcagg aggtagtgcg
     3121 ccgtccggac ggcggcttcc gcgacgcccc ccgacgcccc gaacagcttg cccgccgacg
     3181 tgcgctcgcc gaacggcgtg tcggcggcgt ccggcggcag cgcggacagg tcgaggccgt
     3241 acatgcggat gagttcggcc agctcgcgcg tcgtgaggac cgcgtcgatg tcctgctggc
     3301 cggcgcggcc catctcctcg cgccccgcct cgaacttctt ggccgtgcac ggcatgatgg
     3361 ccacgctgta gatccgggcc ggatcgaggc cgaggcgctc cgccaggaac gtcttgacga
     3421 tggcgcccaa catctgctgc gggctcttgc aggtggacag gttcggcagc aggtccgggt
     3481 agaactgctc catgaacttg acccatccgg ccgagcagct cgtcatcatg gggagcaccc
     3541 cgcccgtcgt cagccggtgg gccagctccg aggcctcttc catgatcgtc aggtcggcgc
     3601 tgaacgacgt gtcgaacacc caggcgaacc cgagccggcg cagcgccgtg accagcgggc
     3661 cgtccacgtc cgtgcccggc ttgaggccga actcctcggc caggctgacc gacaccgacg
     3721 gcgcgtgctg aaccacgacg atccgcgacg ggtcggccag cgccgcccgc acgtcgtcga
     3781 tggcgcgcgt ctcgaccagg gcgccgaccg ggcagaccgc cacgcactgg ccgcagttga
     3841 cgcagctgct cacgttcagg ccctcgtcga aggccgttcc gatccgcgtc ttgctgccgc
     3901 gcccgatgaa gtcgatggcg ccgaccgact gcacttcttc gcacacccgg acgcacttgc
     3961 cgcacaggat gcacttggag ggatcccgcg cgatcgacgg gctcgcgatg tcgaggcggc
     4021 ccggcgtgcg gccgccgaag taccggcgtc ggcggacgcc gagctgctcg gccagctcct
     4081 gcagctggca ctcgccgttg cgcgcgcagt acaggcagtc gtccgggtgg ttggcgagca
     4141 gcagctccac cagcgtccgg cgcgcgtcga cggcgcgcgg cgagtgcgtc tgcaccttga
     4201 ggccgtccga cgccgggaac gcgcagctcg gcacgaggcc ccgctgcccc tcgatctcga
     4261 ccacgcacat gcggcacgcg
//
LOCUS       DAOSIF010000255         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18254_length_4269_cov_3.418130, whole genome shotgun sequence.
ACCESSION   DAOSIF010000255 DAOSIF010000000
VERSION     DAOSIF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18254_length_4269_cov_3.418130"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLT35_09025"
     CDS             <1..706
                     /locus_tag="PLT35_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29410.1"
                     /translation="RMEGLLAMRRRAEALVGSLGPDNAVAVASFQSHLELWTDFTTDR
                     EALGAILGRAVLASGRPPDEAGPAPSGSLRAVFDRAAGRRAATMEQALLVLARALDGV
                     PGPKALVLLGHGFGRIVSPAAWGALMVGFDDEYDRARRILSRARVTVYCLDLTQADAH
                     TLEAGLVRIAEDTGGFYARTHVFPDRAFASLGEALTGHYEVTFETPRLPWGEHRIRID
                     LVGRQGVVRARRFYVG"
     gene            complement(727..2442)
                     /locus_tag="PLT35_09030"
     CDS             complement(727..2442)
                     /locus_tag="PLT35_09030"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOG29411.1"
                     /translation="MPVRRLVALSAALAALPVSLLVGLLVGARPASASRPSRAEQFDV
                     RILVEPGGSIRVTETIRFSFGPKPLTSVRREVARHRTDGLTVAEATMDGVPLARGTKA
                     GQFEWRREDKGKRQIVWHFKPASNSVHTFTVTYVAAGVAWQDAGSDVLAWPLVPSARA
                     FDIECASGEVEFPASATLLGTPGLTPPASQMQVGERRVSFTRCPLEPNRSWIVTLRFA
                     PGTLLASPPAWQAKANRGRAHLPLFLGLAAMMLVGGVGGFTAFALGHRAASGGDADRR
                     LQTPPDDLPPALAGALLRPASAAGWSDLLGAVMDLGRRGVIHIEAKPSSGVFRQHDAT
                     LSRGDAPSGLLAHEQALLDIFLTDKSGPRSSVTFSQIARSVSSPSRWKRLQAAVSEDL
                     REARLLDPERERTRGRVIGLGAAILALAFVGFVLSVAFIERAGEPVLALPIAALIVGI
                     TGIVTGAHLSPLSEEAIGRAERWRAYRRYLADSSTRAGTTARSFERVLPYAAAFGLAL
                     GWAKRLQNQGVTDGPSWMVAAAKDGTAVPADIGDVVAVLSAGSRAGNRAGRSDAGVAH
                     AGGAG"
     gene            2622..3815
                     /locus_tag="PLT35_09035"
     CDS             2622..3815
                     /locus_tag="PLT35_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG29412.1"
                     /translation="MSDLRIEEHAREGGQYRAGARIFAFVLLAACGAAAWYGLRAPRP
                     AAVRVAVVTEAAGGAPPVLNASGYVTARRRATVSSKITGKVTGVYVEEGMAVKAGQVL
                     ARLDDSIPRRYLELAEAELAATRRQEAEVAVRLKEARQNLDRARALVADGIAGQAELD
                     KAEAEADAYQARLALIREQVTVQERQVGVRRTELDDAVIRAPFSGIAISKDAQPGEMV
                     SPVSAGGGFTRTGISTIVDMSSLEIEVDVNESYINRVAPGQAVEAVLDAYPDWRIPSH
                     VIAIVPTADRQKATVLVRIGFERLDARILPDMGVKVAFLGAAGAGAANPAPRVQMPRS
                     AVRTDHGQSVTFVVAGDRVERRAVRIGEARGDLIGVVSGLAAGEQVVVEGPADLADGR
                     RIRIR"
     gene            3829..>4269
                     /locus_tag="PLT35_09040"
     CDS             3829..>4269
                     /locus_tag="PLT35_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG29413.1"
                     /translation="MSDEARADGVLVRIRGLHKVFRRGDERIDVLHGLDLDIPRGDFL
                     ALMGPSGSGKSTLLNLIGGLDRPTLGAIDVDGQRIDGMSGGRLAAWRARHVGFVFQLY
                     NLLPVLTAERNVELPLLLTGLSGRQRKRHVAAALAIVGLTDRARH"
BASE COUNT          611 a         1489 c         1588 g          581 t
ORIGIN      
        1 ccggatggag gggctcctcg ccatgcggcg gcgtgccgag gcgctggtcg gcagcctcgg
       61 ccccgacaac gccgtggcgg tggcgtcgtt ccagtcgcac ctcgagttgt ggacggactt
      121 cacgaccgac cgggaggcgc tcggcgccat cctcgggcgc gccgtcctgg cctcgggccg
      181 gccccccgac gaggcggggc cggcaccgtc cggttcgctc cgagccgtct tcgaccgtgc
      241 cgcagggcgc cgagccgcga ccatggaaca ggcgctcctt gtcctcgccc gggcgctcga
      301 cggcgtaccg ggcccgaagg cgctcgtgct tctcggccac gggttcggcc gcatcgtcag
      361 cccggcggcg tggggggcct tgatggtggg tttcgacgac gagtacgaca gggccaggcg
      421 catcctctcg cgcgcccgcg tgacggtgta ctgcctggac ctgacccagg cagacgccca
      481 caccctagag gccggcctcg tccggatcgc cgaggacacc ggcggcttct acgccaggac
      541 gcatgtgttc ccggaccggg cctttgccag ccttggcgag gcgctcaccg gccactacga
      601 ggtcaccttc gagacgccgc ggctgccgtg gggtgagcat cgcattcgga tcgatctcgt
      661 gggccgccag ggagtggtgc gggcgcgcag gttctacgtc ggctagcgcc ggaacctcca
      721 gcctgctcaa cccgcgcctc cggcgtgggc gaccccggcg tcagagcggc cggcgcgatt
      781 gccggctctc gatccggccg acagcacggc cacgacgtcg ccgatgtccg ccggaaccgc
      841 cgtgccgtcc tttgcggcgg ccaccatcca ggacggcccg tccgtcacgc cctggttctg
      901 cagccgcttc gcccagccca gggcgaggcc gaatgccgcg gcgtagggca gcacgcgctc
      961 gaacgaacga gccgtcgtgc cagcccgggt ggaggagtcg gcgaggtagc gccggtaggc
     1021 gcgccagcgc tcggcccggc cgatcgcctc ctccgacaac ggagacaggt gcgcgccggt
     1081 cacgatgccc gtgatcccga cgatgagcgc ggcaatcggc agggccagca cgggctcccc
     1141 ggcccgctcg atgaacgcga cgctcaggac gaacccgacg aacgccaggg cgaggatggc
     1201 ggcaccgagg ccgatgacgc gtccgcgcgt ccgctcgcgc tcgggatcca gcagcctcgc
     1261 ctccctgagg tcctcgctga ccgcagcttg aagacgcttc caccggctgg gggaggacac
     1321 gctgcgggca atctgcgaga acgtgacgct cgatcgaggc ccggacttgt cggtgaggaa
     1381 gatgtcgagc agcgcctgtt cgtgcgcgag caggccggaa ggggcgtcgc cccggctgag
     1441 cgtggcgtcg tgctgcctga agacgccgct cgagggcttc gcctcgatgt ggatgacccc
     1501 gcgccggccg aggtccatca cggcgccgag caggtcgctc cagcccgccg ccgaggccgg
     1561 cctcagcagc gcgccggcga gagcgggcgg aagatcgtcc ggcggcgttt gaaggcggcg
     1621 gtcagcgtct ccacccgagg ccgcccggtg gccgagggcg aacgccgtga accccccgac
     1681 gccgccgacc agcatcatgg ccgccaggcc gagaaagagc ggcaggtgcg cgcggccgcg
     1741 attcgccttc gcctgccacg ccggtgggga ggccagcagc gtccccggcg cgaagcggag
     1801 cgtgacgatc cacgagcggt tcggttcgag cggacagcgc gtgaaggaca cgcggcgctc
     1861 cccgacctgc atctggctgg ccggcggggt gagtccgggt gttccgagca gcgtcgccga
     1921 ggcggggaac tcgacctcgc cggaggcgca ctcgatgtcg aacgcacggg cggacggcac
     1981 cagcggccag gcgagcacgt cgctgccggc gtcttgccac gccacgccgg cggcgacgta
     2041 cgtcacggtg aacgtgtgga ccgagttcga cgccggcttg aagtgccaga cgatctgccg
     2101 cttgcccttg tcttcgcgcc gccactcgaa ctggccggcc ttggtgcctc tggcgagcgg
     2161 gacgccatcc atcgtagctt ccgccaccgt caatccgtcc gtccggtgcc gcgccacttc
     2221 ccggcggacg gaggtcaagg gcttcggccc gaacgagaag cggatggtct cggtgacgcg
     2281 aatcgacccg ccaggctcga cgaggatgcg cacgtcgaac tgttcggcgc gggagggtct
     2341 ggaggccgaa gccgggcggg cgccgacgag caggccgacg agcaggctga cgggcagagc
     2401 ggcgagcgcg gcggagaggg caacaaggcg ccggacaggc atgccagcct ccagtggttt
     2461 cattatgata tcagtacgaa atcccggtgt ccagcccgcg agcggagccg ccggcgccgg
     2521 cgtcgcccga tccggcgggg ttccgcgagc ggcgcgtgac gcagcgccgc gccgacgcta
     2581 gaatgagcgc gtcgatcccc atgagccagg acaggccgtc cttgagcgac ttgcgaatcg
     2641 aggagcacgc gcgcgagggc gggcaatacc gcgccggcgc gaggatcttc gccttcgtgc
     2701 tgcttgctgc ctgcggcgct gcggcgtggt atgggctccg cgccccccgc cccgcggccg
     2761 tgcgcgttgc ggtcgtcacc gaggccgccg gaggcgcgcc tccggtgctg aacgcctcgg
     2821 gctatgtgac cgcgcggcgc cgcgccacgg tgtcgtcgaa gataacgggg aaggtcaccg
     2881 gggtctatgt ggaagaaggc atggcggtga aggcgggcca ggtcctcgcc cgcctcgacg
     2941 actcgatccc gcgccgctac ctggagctcg ctgaggcgga gctcgccgcc actcggcggc
     3001 aggaagcgga agtcgcggtc cgtctcaagg aagctcgtca gaacctcgat cgtgcccggg
     3061 ccctcgtggc cgacggcatc gcgggacagg ccgagctcga caaggccgag gccgaggcgg
     3121 acgcctatca ggcgcgcctc gcgctcatcc gggagcaggt gacggttcag gagcgccagg
     3181 tgggcgtccg ccgcaccgaa ctcgacgacg cggtgatccg cgcgccgttt tccggcatcg
     3241 ccatctcgaa ggacgcgcag cctggcgaga tggtgtcgcc cgtctcggcg ggcggcggct
     3301 tcacgcgcac cggcatctcg acgatagtcg acatgtcgtc gctcgaaatc gaggtggacg
     3361 tcaacgagag ctacatcaac cgcgtggcgc ccgggcaagc ggtggaagcg gtgctcgacg
     3421 cgtacccgga ctggcgaatc ccgtcgcatg tcatcgcgat cgtgccgacg gcagatcggc
     3481 agaaggcgac ggtgctggtc aggatcgggt tcgagcggct cgatgcccgc atcctgccgg
     3541 acatgggcgt gaaggtggcg tttctgggtg ccgccggggc cggagctgcg aacccggctc
     3601 cgcgggtcca gatgccccga tcggcggtcc gcaccgacca cggccagagt gtgacgttcg
     3661 tggtcgccgg tgatcgggtc gagcgccgcg ccgtccggat cggcgaggcg cgcggcgacc
     3721 tcatcggcgt cgtgagcgga ctcgcggccg gagagcaagt ggtggtggaa gggcccgcgg
     3781 accttgccga tggccgccgg atccgcatcc ggtaggcggg gaggcgccat gagcgacgag
     3841 gcgcgcgccg acggcgtgct ggtgcgtatc cggggcctgc acaaggtctt tcgccggggc
     3901 gacgagcgga tcgacgtcct gcacggcctc gacctcgaca tcccgcgcgg cgacttcctc
     3961 gccctcatgg ggccgtcagg gtcggggaag tcgacgctcc tcaacctgat cggcggcctg
     4021 gatcggccga ccctcggcgc gatcgacgtg gacgggcagc gcatcgacgg gatgtcgggc
     4081 ggccggctgg ccgcgtggcg cgcccgccac gtcgggttcg tcttccagct ctacaacctg
     4141 ctgccggtgc tcacggccga gcggaacgtc gagttgccgc tccttctgac gggcctgtcg
     4201 ggccggcagc ggaagcggca tgtcgccgcg gcgctcgcca tcgtgggcct gaccgatcgc
     4261 gccaggcac
//
LOCUS       DAOSIF010000256         4264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18290_length_4264_cov_3.506058, whole genome shotgun sequence.
ACCESSION   DAOSIF010000256 DAOSIF010000000
VERSION     DAOSIF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18290_length_4264_cov_3.506058"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLT35_09045"
     CDS             complement(<1..1000)
                     /locus_tag="PLT35_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG29414.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPVVITNPEGGRLTPSVVAFTK
                     TGERLVGQVAKRQAVTNPENTVFSIKRFMGRRYEEVSEEMKMVPFRVGRDGDHVVVQV
                     QSAGKEERLPPPQISAMVLQKLKKAAEDYLGQPVSKAVITVPAYFNDAQRQATKEAGV
                     IAGLEVMRIVNEPTAAALAYGLDKKKEETIAVYDFGGGTFDISILEVGEGVVEVKATN
                     GDTHLGGDNLDQEVMDWIIAEFKKSDGIDLSRDRMALQRLREAAEKAKMELSTVMETE
                     INLPFITADASGPKHLQMKLTRAKFEQLVDDLLQKTIGPVKQALADAGLQPSQVNEV"
     gene            1253..3304
                     /locus_tag="PLT35_09050"
     CDS             1253..3304
                     /locus_tag="PLT35_09050"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOG29415.1"
                     /translation="MPTLRRAGFLVSCCVVLAARAGAQTAEPHAPNHDAPLHLSVVEG
                     QVFLDREERTETAVANVPLLAGDRLRTEAGRAEVVCPDGSLLHVDRATVVDVMATDLL
                     RLLEGRLALTVHGAGAPDEAVRYQIDAPAASVQTRGPGEFRISSMADGAVSEVELAVF
                     RGEAVLANDAGAQAVRAGELAVARDDLAPSRPQYFNSARWDEFDAWSARRRQAARSAV
                     SVDDLPPDLAPYAATFDRYGVWRDDPIDGRVWYPTVAADWRPYSVGYWRSYPAWDAFW
                     VAGDPWGWPTHHYGRWGFSHTFGWYWMPARVWGPSWVYWAYAPGYVSWCALGRWNNPV
                     FGLFGLLGSIVGNRVDPWRGWNIIERRHYGSFQSMHRVAFDGRRLGRDDYQRFAVQRR
                     APGWAPGERGGPVPGTPDRQRDVRGPDGRGGAQRPAWPTVPRRSFANAEPRSGAAPRT
                     QPARPTPDVTAFSNRRTGPAERPSLGSAASSGSTRAPSSRSLGSALGSALGTAPRPAY
                     QRTPSAAAGSSRPLPSSRSLGSALGTAPRPAYQRAPAAGAGSSRPLPSSRSLGSALGT
                     APRPAYQRAPAAGAGSSRPLPSSRSLGSAFGTAPRPAYQRAPAAGAGNSSPLPSSRSL
                     GSAFGSAPRAAVPRGGSMGSGSSSSRAPASRYNPGSGGARQPGGGAASARPRGGRTPA
                     P"
     gene            3308..>4264
                     /locus_tag="PLT35_09055"
     CDS             3308..>4264
                     /locus_tag="PLT35_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG29416.1"
                     /translation="MAKPRSSRSSLARQATVVALFVLAAFLGILTGVLLAYTGDLPLV
                     SALDEYTPSTITRVLAKDGRVIGEYATQRRVILKYEEIPAVMRQAILAAEDAEFERHI
                     GIRPVRLLVAVLKNLLVQHRLMGGGASTLTQQLARKLFLKPDKTLERKVKEWVLALQI
                     EKRYTKREIFTLYCNQMNLGHGAYGVEAAARVYFGKPVKDLRLEEAAMIAGILQLPER
                     QSPYINQRWATQRRNYTLGRMAAEGFITEAQAEAAKKRPIVLAGLPLPANSKAPYFVE
                     EVRQHLERRYGARELYEAGLTVQTTLDADLQDAATRALDRGLR"
BASE COUNT          583 a         1552 c         1437 g          692 t
ORIGIN      
        1 cgacttcgtt cacctgcgag ggctgcagcc ccgcgtcggc cagcgcctgc ttcaccgggc
       61 cgatcgtctt ctgcagcagg tcgtccacca gctgctcgaa cttcgcccgg gtcagcttca
      121 tctggaggtg cttcggcccg ctggcgtcgg cggtgatgaa cggcagattg atctccgtct
      181 ccatcaccgt ggaaagctcc atcttggcct tctccgccgc ctcccgcagc cgctgcagcg
      241 ccatgcggtc tcggctcaga tcgatgccgt cgctcttctt gaactcggcg atgatccagt
      301 ccatcacctc ctggtcgaga ttgtcgccgc cgaggtgcgt gtcgccgttg gtggccttga
      361 cctcgaccac gccctcgccc acctcgagaa tcgagatgtc gaaggtgccg ccgccgaagt
      421 cgtagacggc gatggtctct tccttcttct tgtcgaggcc gtaggccagc gcggcggccg
      481 tgggctcgtt cacgatgcgc atcacctcga ggcccgcaat gaccccggct tccttcgtgg
      541 cctggcgctg cgcgtcgttg aagtacgccg gcacagtgat gacggccttc gacaccggct
      601 gcccgaggta gtcctccgcc gccttcttga gcttctggag caccatggcc gagatctggg
      661 gcggaggcag gcgctcctct ttcccggccg actggacttg caccaccacg tggtcgccgt
      721 cgcgccccac gcggaacggc accatcttca tctcctcgct gacttcttcg tagcgccggc
      781 ccatgaaccg cttgatcgaa aagaccgtgt tctcagggtt cgtcacggcc tggcgcttgg
      841 ccacctgccc gaccaggcgt tcaccggtct tcgtgaaggc cacgacggac ggggtgaggc
      901 gcccgccctc cgggttggtg atgacgacgg gctcgccgcc ctccatgacg gccacgacgg
      961 agttggtcgt gcccaggtcg atgccaatga ttttgctcat atgtgcttcc tctgcggcag
     1021 tctgttcgac gcgctcgcat cccagcccac tggctgacga tgcttatatt gcccatagaa
     1081 tattatctca gtcgccatta gtcaagtact tgttgatgca acagacctcc ctctcgaagc
     1141 aagtcctgtt ctgtcaattg attctcctga gggaagtgct atactgtcca cgcctgccaa
     1201 ccgccgagat gccccatctg cccggggacg tcctctcggc cgcgaggcga acatgcccac
     1261 gttgcgccgt gcaggcttcc tggtgtcttg ctgtgtcgtc ctcgcagcgc gcgccggcgc
     1321 ccagaccgcc gagcctcacg ctccgaacca cgatgcgcct cttcacctct ccgttgtcga
     1381 gggccaggtg ttcctcgacc gcgaggagcg gaccgagacg gccgtcgcca atgtcccgct
     1441 gctcgcagga gatcggctac ggaccgaagc cgggcgagcc gaggtggtgt gtcccgacgg
     1501 ctcactgctc cacgtcgatc gggcgacggt cgtcgacgtg atggcgacgg acctcctccg
     1561 tctgctagag ggtcgactcg ccttgacggt gcacggcgcc ggtgctcccg atgaggcggt
     1621 tcgctaccaa atcgatgccc ccgccgcgtc ggtgcagacc cgcggcccgg gcgagttccg
     1681 catctcctca atggcggatg gagcggtctc cgaagtcgaa ctcgcggtct tccgcggcga
     1741 agccgtgctg gcgaacgatg ccggcgcgca agcggtacgc gcgggtgagc tggcggtggc
     1801 gcgcgacgat ctggcgccca gccgtcccca gtacttcaac tcggctcgat gggacgagtt
     1861 cgatgcgtgg agcgctcgcc ggcggcaggc ggcccgcagc gccgtgtctg tggacgatct
     1921 gccgccggac ctcgccccct acgcagcgac cttcgatcgc tacggcgtgt ggcgcgacga
     1981 tccgatcgac ggccgggtct ggtatccgac ggtggctgcc gactggcggc cctactcggt
     2041 cggctactgg cgcagctatc cggcctggga tgctttctgg gtggcaggtg acccctgggg
     2101 ctggccgacc catcactacg ggcggtgggg cttctcgcac accttcggct ggtactggat
     2161 gccggctcgt gtctgggggc cgtcatgggt gtattgggcg tacgctcccg ggtacgtcag
     2221 ctggtgcgcc ctcggccggt ggaacaaccc ggtgttcggc ctgttcgggc tgttgggctc
     2281 gattgtcgga aaccgggtcg acccgtggcg aggatggaac atcatcgagc gccggcacta
     2341 cgggtcgttc caatcgatgc accgcgtcgc gttcgacggg cgccgcctgg gtcgcgacga
     2401 ctatcagcgc ttcgcggtcc agcgtcgcgc gcctggatgg gcccctgggg agcgcggagg
     2461 cccggtcccg ggcactccgg accggcagcg cgacgtccgc gggccggacg ggcggggcgg
     2521 cgctcagcga ccagcctggc cgacggtgcc ccgccgctcg ttcgcgaacg ccgagccgcg
     2581 gtccggggcg gccccccgca cgcagccggc gcggccaacg ccagacgtca cggcgttcag
     2641 caaccgccgc accgggccgg ccgagcggcc gtctctcggc agcgccgcgt cgagcggcag
     2701 cacccgagcg ccgtcctctc gcagccttgg cagcgcgctc ggcagcgcgc tcggcacggc
     2761 gccgcgcccc gcgtaccagc gcaccccctc ggccgccgcg ggcagcagcc gtccgctgcc
     2821 gtcctcccgc agcctcggca gcgcgctcgg cactgcgccg cgcccggcat accagcgcgc
     2881 ccccgcggcg ggcgcgggca gcagccgtcc gctgccgtcc tcccgcagcc tcggcagcgc
     2941 gctcggcact gcgccgcgcc cggcatacca gcgcgccccc gcggcgggcg cgggcagcag
     3001 ccgtccgctg ccgtcctccc gcagcctcgg cagcgcgttc ggcactgcgc cgcgcccggc
     3061 gtaccagcgc gcccccgcgg cgggcgcggg caacagcagt ccgctgccgt cctcccgcag
     3121 cctcggcagc gcgttcggca gcgcgccgcg cgcggccgtg ccccgcggcg gcagcatggg
     3181 gagcggcagc tcgtcgtctc gcgcaccggc ttcgcgctac aatccggggt ctggtggagc
     3241 gcgccagcca ggcggagggg ccgcatcggc ccggccgcgc ggcggccgca cgccggcacc
     3301 ctgatcgatg gcgaaaccgc gttcctcccg ctcgtcgctg gcccgtcagg ccacggtcgt
     3361 cgccctcttc gtcctcgcgg cgttcctggg gatcctgacg ggcgttctgc tcgcctatac
     3421 gggcgacctg ccgctcgtct cggccctcga cgagtacacg cccagcacga tcacccgcgt
     3481 cctcgcgaag gacggccgcg tgatcggcga gtacgcgacg cagcgccgcg tgatcctgaa
     3541 gtacgaggag atccccgcgg tgatgcgcca ggcgatcctg gcggctgagg acgccgagtt
     3601 cgagcgccac atcggcatcc gaccggtgcg cctgctcgtc gccgttctca agaacctgct
     3661 cgtccagcac cggctgatgg ggggcggggc gagcacgctc acgcagcagc tggcccgcaa
     3721 gctctttctg aagccggaca agacgctcga gcgcaaggtg aaggagtggg tgctcgcgct
     3781 gcagatcgag aagcgctaca cgaagcgcga gatcttcacc ctctactgca accagatgaa
     3841 cctcggccac ggcgcctacg gggtcgaggc ggccgcgcgc gtgtacttcg ggaagccggt
     3901 caaggacctc aggctggagg aggccgcgat gatcgcgggc atcctgcagc tgcccgagcg
     3961 ccagagccct tacatcaacc agaggtgggc gacgcagcgc cgcaactaca ccctcggccg
     4021 catggctgcg gaagggttca tcaccgaggc ccaggcggag gcggcgaaga agcggccgat
     4081 cgtgctggcc ggcctgccgc tcccggcgaa ctcgaaggcc ccgtacttcg tggaggaggt
     4141 gcgccagcac ctcgagcgcc ggtacggcgc acgcgagctg tacgaagccg gcctgaccgt
     4201 tcagacgacc ctcgatgccg acctgcagga cgcggcgacg cgcgcgctcg accggggcct
     4261 gcgg
//
LOCUS       DAOSIF010000257         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18347_length_4253_cov_4.767508, whole genome shotgun sequence.
ACCESSION   DAOSIF010000257 DAOSIF010000000
VERSION     DAOSIF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18347_length_4253_cov_4.767508"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLT35_09060"
     CDS             complement(<1..259)
                     /locus_tag="PLT35_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG29417.1"
                     /translation="MLTSFMTPFMGAAVNLALPRLAAELGLDAVMLSWIATAYILAAV
                     VFLLPFGRLADIHGRRRVFLSGLTCFLAGSVVAALARTGGML"
     gene            complement(335..1660)
                     /locus_tag="PLT35_09065"
     CDS             complement(335..1660)
                     /locus_tag="PLT35_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOG29418.1"
                     /translation="MSNTVVRCSVVAALGGFLFGFDTVVISGAEQAIQRLWGLRAALH
                     GLVISAALWGTVAGALAGGWPTEHWGRRRTLFAAGVLYFVSAAWSGLATGVYALMFAR
                     FIGGIGVGISTVAAPLYIAEISPASARGRLAGMFQFNIVFGILAAYLSNALLGGVGRH
                     AWRWMLGVEAFPALLFTVLSLWLVESPRWLITHQGDRADGLRVFRRINPALPDADLEA
                     LADEVEASALRGEAGARLFTRRLARPILLAFLIAFFNQFSGVNAILYFAPRIFELTGL
                     AERAALLQSVGVGATNLIFTFAGLWLIDRLGRRTLLTMGSFGYIASLGLCAWAFHSGR
                     VSIVPACIFAFIAAHAVGQGAVIWVFISEIFPNRNRAAGQTLGSSTHWICAALITLVF
                     PKLVEAFQPWAIFAAFCGMMVLQLLWVRLFVPETMGVPLERMPQTLGLD"
     gene            complement(1734..3431)
                     /locus_tag="PLT35_09070"
     CDS             complement(1734..3431)
                     /locus_tag="PLT35_09070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOG29419.1"
                     /translation="MVAAPARERLLDEVFDFRPGEGLIRLREQRVVILSAAAMGLLRK
                     ELIQTLGIETARRVLLRFGFADGYHDAINLKDQLGWSDPLDGVRAAATIHALEGIVRA
                     EFLRLDHDPATGAFGCELAWQRSYEGEQHLEHYGRGSQPVCWSLVGYISGYVSASLGA
                     EIYFRETRCVGLGHGECRLVGSHAGGWGEDLAGFRFDFQGADLGAEVERLRVAATRQR
                     QALAARERALVRREREMDALQQRVSEHVARQRFVVRSPQMQSVLDLASRVAPLDTTVL
                     VTGESGTGKEFIVRLVHAHSARAGGPLVSVNCAALTETLLESELFGHVRGAFTGAVRD
                     KAGLFEVATDGTLFLDEIGEMAPTIQAKLLRALQEGEIRRVGSERDIKVRPRIITATN
                     RDLRATVAEGRFREDLYFRLAGFVIAIPPLRERTDDIPALAQEFLRRTASRLAKDAKA
                     LSSQAMIALVSYPWPGNVRELEHAVERAVILSRGPVLSLDDLPPEVRGGAPRAAGRGD
                     LDLESFRRAMLEEALRRFPRSRKAAAAALGVSTVTLWRMMVRYGLAGSERRGGRSGV"
     gene            3855..4235
                     /locus_tag="PLT35_09075"
     CDS             3855..4235
                     /locus_tag="PLT35_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29420.1"
                     /translation="MQLKLKSPSFTENPLLVQQVERRARLEFGRYGGRVDRVTISVLD
                     RTEAAPIRFHCGVGVHVLDENNDLRLVVSMGEDNDLYRSVDSALTRAGVRTGGEIARA
                     EEALRARRQWLAGLQAHAGTELSS"
BASE COUNT          678 a         1513 c         1494 g          568 t
ORIGIN      
        1 cgagcatccc gcccgtgcgg gcgagcgcgg cgaccaccga cccggccagg aagcaggtca
       61 ggcccgagag gaagacgcgg cggcgcccgt ggatgtcggc gagccgcccg aacgggagca
      121 ggaacacgac ggccgccagg atgtaggccg tggcgatcca gctgagcatg accgcgtcga
      181 ggcccagctc ggcggccagc ctcggcaggg cgaggttcac cgcggcgccc atgaagggcg
      241 tcatgaagga cgtcagcacg gccaccgtca gggtggctcg ctgaagggcg ctcggcgaca
      301 tcatccggtt ccggagcggc ggcctcgcgc cgcctcagtc gagccccagc gtctgcggca
      361 tccgctcgag gggcacgccc atcgtctcgg gcacgaagag ccgcacccac agcagctgca
      421 ggaccatcat gccgcagaag gcggcaaaga tggcccaggg ctggaacgcc tccaccagct
      481 tcgggaagac cagcgtgatg agcgccgcgc agatccagtg cgtggagctg ccgagggtct
      541 ggccggcggc gcggttccgg ttcgggaaga tctcgctgat gaagacccag atgacggcgc
      601 cctgcccgac ggcgtgcgcc gcgatgaacg cgaagatgca ggcgggcacg atggacacgc
      661 gccccgagtg gaaggcccag gcgcagagcc cgagcgaggc gatgtatccg aacgacccca
      721 tcgtcagcag cgtgcggcgc cccagccggt cgatgagcca caggccggcg aaggtgaaga
      781 tcaggttcgt cgcgcccacg cccaccgact gcaggagcgc cgcgcgctcg gcaaggccgg
      841 tgagctcgaa gatgcgcggc gcgaagtaca ggatcgcgtt gacgcctgag aactggttga
      901 agaaggcgat gaggaacgcg agcaggatcg gccgggcgag ccgcctggtg aacaggcgcg
      961 cgccggcctc gccgcgcagg gccgacgcct cgacctcgtc ggcgagcgcc tcgaggtcgg
     1021 cgtccggcag ggccgggttg atgcggcgga agacgcgcag cccgtccgct cggtcgccct
     1081 ggtgggtgat gagccagcgc gggctttcga ccagccacag gctgagcacc gtgaacagga
     1141 gggctgggaa ggcttcgacg ccgagcatcc agcgccacgc gtgccgtccg acgccgccga
     1201 gcagcgcgtt cgacaggtag gccgcgagga tgccgaagac gatgttgaac tggaacatgc
     1261 cggcgaggcg gccgcgcgcg gaggcggggg agatctcggc gatgtagagc ggcgcggcca
     1321 cggtcgagat gccgacgccg atgccgccga tgaagcgcgc gaacatcagc gcgtacacgc
     1381 ccgtcgcgag gcccgaccag gcggccgaga cgaagtagag gacgccggcc gcgaagagcg
     1441 tccggcggcg gccccagtgc tcggtgggcc agccgccagc gagcgccccg gccaccgtgc
     1501 cccagagggc ggcgctgatg accaggccgt gcagcgccgc gcgcaggccc cacagccgct
     1561 ggatggcctg ctcggcgccc gagatgacga ccgtgtcgaa gccgaacagg aagccgccca
     1621 gcgcggcgac tacggaacac cgcacgaccg tgttgctcat ccccgttcct gcctgcgcgc
     1681 ggggccccgg cgcgggcccg ccgacccggg ctcccccgcg cgtgcgccgc cgatcatacg
     1741 ccgcttcggc cgccgcgtcg ttcgctgccg gccaggccgt agcggaccat catccgccac
     1801 agcgtgaccg tgctgacgcc gagggccgcc gccgccgctt tccggctgcg cgggaagcgc
     1861 cggagcgcct cctccagcat ggcccgccgg aaggactcga ggtcgaggtc gccgcggccg
     1921 gcggcgcgcg gggcccctcc gcgcacttcg ggcggcaggt cgtcgaggga cagcacgggt
     1981 ccgcgcgaga ggatgaccgc ccgctcgacc gcgtgctcga gctcgcggac gttgccgggc
     2041 caggggtagc tcaccagggc gatcatcgcc tgcgacgaca gcgcctttgc gtccttcgcc
     2101 aggcgcgagg ccgtccgccg gaggaactcc tgcgcgagcg ccgggatgtc gtcggtccgc
     2161 tcgcgcagcg gcgggatcgc gatgacgaac cccgcgaggc ggaagtacag gtcctcgcgg
     2221 aagcgcccct cggcaaccgt ggcccggagg tcgcggttcg tggcggtgat gattcgcggg
     2281 cggaccttga tgtcgcgctc gctgccgacg cgccggatct caccctcctg cagcgcccgc
     2341 aggagcttcg cctggatggt gggggccatc tccccgattt cgtcgaggaa cagcgtgccg
     2401 tcggtcgcca cttcgaacag gcccgccttg tcgcggacgg cgccggtgaa cgcgccacgg
     2461 acgtggccga acagctccga ctccagcagc gtctcggtca gcgcggcgca gttcaccgag
     2521 accagcggcc cgccggcccg cgcgctgtgc gcgtgcacca ggcggacgat gaactccttg
     2581 cccgtgccgc tctcgcccgt cacgagcacc gtcgtgtcca gcggcgccac gcgcgaggcc
     2641 aggtccagca cgctctgcat ctgcgggctg cgcacgacga agcgctggcg cgccacgtgc
     2701 tccgacaccc gctgctggag cgcgtccatc tcgcgttcgc ggcggaccag cgcgcgctcc
     2761 cgcgcggcca gcgcctggcg ctggcgcgtg gccgccacgc gcaggcgctc gacttcggcc
     2821 ccgaggtcgg cgccctggaa gtcgaagcgg aagccggcga gatcctcccc ccagcctccc
     2881 gcgtggctcc cgacgaggcg gcactcgccg tggccgaggc ccacgcacct cgtttcccgg
     2941 aagtagattt ccgcgccgag cgacgcgctg acgtacccgc tgatgtaccc gaccaggctc
     3001 cagcacaccg gctggctgcc gcgcccgtag tgctccaggt gctgctcgcc ctcgtacgag
     3061 cgctgccacg cgagttcgca tccgaaggcc cccgtcgccg ggtcgtggtc cagcctgagg
     3121 aactcggcgc ggacgattcc ctcgagcgcg tggatcgtcg cggctgcccg caccccgtcg
     3181 agggggtcgg accagccgag ctggtccttg aggttgattg cgtcgtggta gccgtcggcg
     3241 aagccgaacc gcagcaggac gcggcgcgcg gtttcgatgc ccagcgtctg gatgagttcc
     3301 ttccgcagca ggcccatcgc ggcggcgctc aggatgacga cccgctgctc gcggagcctg
     3361 atcaggccct cgcccgggcg gaaatcgaag acctcgtcga gcaggcgctc acgggccggt
     3421 gccgcgacca taccaccctc cccaccggcc tggcgccggc tctgaaaatt gcattatgaa
     3481 ataatagctg tcgaccggca agctcccgtc ggcttcccgg caggaattgc accgctaacg
     3541 ctcgaaaaca cgctgaaaac aggtatcttg cgacgctgat cctgccgatt cggtatcctg
     3601 cttcggcatt cagtgagttt caatatgata tgattacttt ctaattgaaa ttttcggcag
     3661 cctgtgcgcg ctgtgtgcag tccgcacaag ttcctcaaag gcaatcagtt gcgccgagcc
     3721 tgcctgcctg cacaggttgg ccggccgctt gcgactgttc gcagcagcgg ccttctgccg
     3781 caggaggtcg cgtgtgtccg ctgcaaccac gcccggttgg gcacccgctc cctctcaatt
     3841 caccggagac tgacgtgcag ctgaaactca agagcccttc gttcaccgag aacccgctgc
     3901 tcgtccagca ggtcgaacgc agggcccgcc tggagttcgg ccgctacgga ggacgggtgg
     3961 atcgggtcac gatctccgtg ctggaccgca ccgaggccgc gccgatccgc ttccattgcg
     4021 gtgtcggcgt gcacgtcctc gacgaaaaca acgacctgcg cctcgtggtc tcgatgggcg
     4081 aggacaacga cctttaccgc tcggtcgatt cggccctcac gcgggccggc gtgcgaacgg
     4141 gcggcgagat cgccagagcc gaagaggcgc tccgcgcaag gcggcagtgg ctcgccggcc
     4201 tccaggccca cgccggaacg gagctgtcct catgaacccc tacctccaac tcg
//
LOCUS       DAOSIF010000258         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18404_length_4242_cov_3.485789, whole genome shotgun sequence.
ACCESSION   DAOSIF010000258 DAOSIF010000000
VERSION     DAOSIF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18404_length_4242_cov_3.485789"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLT35_09080"
     CDS             complement(<1..232)
                     /locus_tag="PLT35_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG29421.1"
                     /translation="MLALETDRLTKDYAVGFWRRRPYRALDGLSLQVEAGEVFGLLGP
                     NGAGKTTTLKLLMELIYPTSGRIAILGHPPGHP"
     gene            complement(236..1459)
                     /locus_tag="PLT35_09085"
     CDS             complement(236..1459)
                     /locus_tag="PLT35_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOG29422.1"
                     /translation="MSTTAMSPAIRRVLALLALAGLASAFGSMLVHVKLVRQPQYLSF
                     CDVTSYISCSTVYQSRYAELLGVPVALLGALWYVAVLVVLAGARWGWTSLRDSALGYV
                     FLLSVAGFGFVLYMAYASLVLLKTICLLCVATYVCVASIFVISGLKTVPPMTSIPRRL
                     LRDARAALASRGAVATVLAFLVLATTAVAFFPRLPVKEAAAAVGQGGAAAPADQQSEF
                     LRFWEAQPRVPVPVSSEGAAVLVVRFSDFGCPSCGQTYEAYKSVFAKYAAQHPGAIKL
                     VAKDFPLEPECNSNVGRPMHPSSCEAAVAARLAKAAGRGEAMEDWLYKNSAYVPPASI
                     RQAAQEIGGVRDYDAKYAATINEIKGDIALATVLGVRVTPTFFINGVKLEGGLPPQYF
                     DLALEQELKRAGKRP"
     gene            complement(2092..2175)
                     /locus_tag="PLT35_09090"
                     /pseudo
     CDS             complement(2092..2175)
                     /locus_tag="PLT35_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(2431..3861)
                     /locus_tag="PLT35_09095"
     CDS             complement(2431..3861)
                     /locus_tag="PLT35_09095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG29423.1"
                     /translation="MTMMTTNETSAPNAPRSGRILVVDDERSMRELLQIVLRREGHQV
                     RLAEDGPAAVAELGREPADVLISDIRMPGMSGVDVLREAKRLDPDIVGIMVTAYASTD
                     SAVEALRLGAYDYLTKPFDVEELKAKVRNALERRTLRQENVLLKRALRSSSSFSNIVG
                     RSRVMQDVFDLVETVAPTNSTILIMGESGTGKELVARAVHTNSLRRDQPFVALNCGAL
                     PEALLESELFGHMRGAFTGAAATKKGLLEVAERGTVFLDEISEMSPMMQVKLLRVLQE
                     RRFRRVGGNEEIDADIRIIAATNRDLARAVADGTFREDLYYRVNVIPIHLPPLRERRE
                     DIAPLAEHFVAKYREQMGKAVSGLTPEAVRWLEAGDWPGNVRQLENVIERAVALERGP
                     LIQIASLPAGPAPRPPSKQGANGALAGAALPEAGLDLPRHLESQERDLVAQALQQAGG
                     RHDRAARLLAITPRQLRYLLDKHALR"
     gene            complement(3858..>4242)
                     /locus_tag="PLT35_09100"
     CDS             complement(3858..>4242)
                     /locus_tag="PLT35_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29424.1"
                     /translation="AVEVDVPSSPVWCEADEGQLRQVVWNLATNGLRAMPDGGRLRLG
                     VRAEPGGASAVLEVRDEGVGIPETELDAVFQPFHSTFERGSGLGMAVVHRIVSDYGGE
                     VRVTSQPGAGTTVEVRIPARALAAA"
BASE COUNT          603 a         1524 c         1512 g          603 t
ORIGIN      
        1 cggggtggcc gggaggatgc ccgaggatcg cgatgcggcc cgatgtggga tagatgagct
       61 ccatcaggag cttcagcgtc gtcgtcttgc cggcgccgtt cggaccgagg agcccgaaca
      121 cctcgcccgc ctccacctgc aacgacaggc cgtcgagcgc gcgataggga cgccggcgcc
      181 agaacccgac ggcgtagtcc ttggtgagcc gatcagtctc cagcgccagc atggctcagg
      241 gacgcttgcc cgcccgcttg agttcctgct cgagggccag gtcgaagtac tgcggcggca
      301 ggccgccctc gagcttcacg ccgttgatga agaacgtcgg ggtgacccgc acgcccagga
      361 ccgtcgccag ggcgatgtcg cccttgatct cgttgatcgt ggcggcgtac ttcgcgtcgt
      421 agtccctgac gccgccgatc tcctgggccg cctggcggat ggaggccggc ggcacgtagg
      481 ccgagttctt gtagagccag tcctccatcg cctcgccccg gccggcggcc ttcgccaggc
      541 gcgccgcgac cgccgcttcg cacgaggagg ggtgcatggg gcgcccgacg ttcgagttgc
      601 actccggctc gagcgggaag tccttggcga ccagcttgat cgctcccgga tgctgggcgg
      661 cgtacttggc gaagacgctc ttgtaggcct cgtacgtctg cccgcaggac gggcagccga
      721 agtccgagaa gcggaccacc agcaccgccg ccccttccga ggaaaccggc accggcaccc
      781 ggggctgggc ctcccagaaa cgcaggaact cgctctgctg atccgccggc gccgccgcgc
      841 cgccctgccc gaccgccgcg gccgcctcct tcaccgggag ccgcgggaag aacgccacgg
      901 ccgtggtcgc caggacgaga aacgccagca ccgtcgcgac ggcgccgcgg ctcgcgaggg
      961 cggccctggc atcgcgcagc agccgccggg ggatggatgt catgggaggc accgtcttca
     1021 gcccggagat gacgaagatg gaggcgacgc agacgtacgt cgcgacgcac aggaggcaga
     1081 tcgtcttgag caggaccagc gacgcgtagg ccatgtacag cacgaacccg aagccggcaa
     1141 ccgacagcag gaacacgtag ccgagggcgc tgtcgcgcag gctcgtccag ccccagcgcg
     1201 cgcccgcgag cacgacgagg accgcgacgt accacagcgc gccgagcagc gccaccggca
     1261 cgccgagcag ctccgcgtag cggctctggt agaccgtcga gcagctgatg tacgaggtga
     1321 cgtcgcagaa gctgaggtac tgcggctggc gcacgagctt cacgtggacc agcatcgagc
     1381 cgaacgcgga cgccaggccg gcgagggcga gcagggcgag gactcggcgg atcgcggggc
     1441 tcatcgcggt cgtgctcaac tatccacttg tagcggcttc gcgcaggaac gtcaagcggg
     1501 gcccccgggg atcaggcgcc cgtccgacgc acgcgaggga tgccggccgg cgtgcggcgg
     1561 gcatccctcg cgcgtgtggc acctcgtggt gcgtcgacga agagcggagg atgcccggcc
     1621 cggacatcct cccgtcgtca ggcaggcagc cggctactgg atcacggtga cggtcccggc
     1681 gggcgcgaac ggctcgcccg gcgcggcgcc gctcgtgtcc tgccagatcg cgccgctctg
     1741 gttcgtcgcg aacccgcggg ccccggtcgt gccgaccgtg agggccaccg ccgacgcgta
     1801 gtagccggtc tgggtcgccg tcccgttgca gtcgttgggg ccggccacgg cgccggcgcc
     1861 acccgccatc gtcacggtgt atccgctctt gaccaccacg tcggccgcgc cgagatcggc
     1921 tgaaatgaac ccctcgccga cgccgttgca cggcagcgcc agggtcgtca gcgcgatcgc
     1981 atagccgcgg ctggcagccg tcgcggcata gctcgcctgc gaactgttga tcgtgcgcat
     2041 cgagccgatg gccgacgcct cgttgccggc catgcgggcg cgcatcaggc ccggaatcgc
     2101 gatggccgcg atgatgccga tgatcgccac gacgatcagc agctcgatga gtgtgaatcc
     2161 cttgttgctc ttcatgcctc gctcccttcg gaaatggaac cggccggtcc gtcctgaccg
     2221 cgtccgccgc agtcagccgg gcgggttcca tgtcccgacg actgcccttt gcccgaggga
     2281 gcagcaaggc cgatgccagc gtgccggcgt cgggccttgt cgctggaaat gcctgacaat
     2341 gcacagggtt gcgcgcgcct gcgccggacc tccccggccg ggcctgccag cccggtgacg
     2401 aagcgcgtca atgggccgtg ccggaaaccg tcaccggagc gcgtgcttgt cgagcaggta
     2461 gcgcagctgg cgcggcgtga ttgcgagcag gcgggccgcc cgatcgtggc gccccccggc
     2521 ctgctgcagc gcctgcgcga ccaggtcgcg ctcctgcgac tcgaggtgcc gcggcaggtc
     2581 caggccggcc tccggaagcg cggcgcccgc cagggcgccg ttggcgccct gcttcgacgg
     2641 cggccggggc gcagggccgg cggggaggct cgcgatctgg atcagggggc cccgctcgag
     2701 ggccaccgcc cgctcgatga cgttctcgag ctggcgcacg ttgcccggcc agtcgccggc
     2761 ctcgagccac cggacggcct ccggcgtcag cccgctgacg gccttgccca tctgctcgcg
     2821 gtacttcgcg acgaagtgct cggccagcgg cgcgatgtcc tcgcgccgct cgcgcagcgg
     2881 cggcaggtgg atggggatga cgttgacgcg gtagtagagg tcctcgcgga acgtcccgtc
     2941 ggcgacggcc cgggccaggt cccggttcgt ggcggcgatg atgcggatgt cggcgtcgat
     3001 ttcctcgttg ccgccgacgc gccggaagcg ccgctcctgc agcacgcgca gcagcttcac
     3061 ctgcatcatc gggctcatct cggagatctc gtccaggaag acggtgccgc gctcggcgac
     3121 ctccaggagg cccttcttgg tggcggccgc cccggtgaac gcgccgcgca tgtgcccgaa
     3181 cagctccgac tccagcagcg cctccgggag cgccccgcag ttgagcgcca cgaacggctg
     3241 gtcgcgccgc agcgagttcg tgtggacggc gcgggcgacc agttcctttc ccgtcccgga
     3301 ttcccccatg atgaggatgg tgctgttggt cggcgcgacg gtctcgacca ggtcgaagac
     3361 gtcctgcatc acccggctcc ggccgacgat gttcgagaag gacgaggacg agcgcagggc
     3421 gcgcttgagg agcacgttct cctggcgcag cgtccggcgc tcgagggcgt tgcggacctt
     3481 cgccttcagt tcctccacgt cgaacggctt cgtgaggtag tcgtacgcgc cgaggcgcag
     3541 ggcctcgacg gccgagtcgg tggacgcgta ggccgtcacc atgatgccga cgatgtccgg
     3601 gtcgaggcgc ttcgcctcgc ggagcacgtc cacgccgctc atgcccggca tcctgatgtc
     3661 ggagatcagg acgtccgcgg gctcccggcc cagctccgcc acggccgccg gcccgtcctc
     3721 ggcgagccgc acctggtgcc cctcccggcg caggacgatc tgcagcagct cgcgcatcga
     3781 ccgctcgtcg tccacgacga ggatccggcc gctgcggggg gcgttcggcg cgcttgtctc
     3841 gttcgtcgtc atcatcgtca tgcggcggcc agcgcccggg ccgggatgcg cacctcgacg
     3901 gtggtgccgg cccccggctg cgacgtgacg cgcacctcgc cgccgtagtc gctcacgatg
     3961 cggtgcacga cggccatgcc gaggccgctg ccccgctcga aggtgctgtg aaagggctgg
     4021 aacaccgcgt ccagctcggt ttccgggatg cccacgcctt cgtcgcggac ctcgaggacg
     4081 gccgacgccc cgcccggctc ggcgcgcacg ccgaggcgca ggcggccgcc gtcgggcatc
     4141 gcgcgcagcc cgttggtcgc caggttccag acgacctggc gcagctggcc ctcgtcggcc
     4201 tcgcaccaca ctggcgagga cggcacgtcc acctcgacgg cg
//
LOCUS       DAOSIF010000259         4239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18426_length_4239_cov_3.154398, whole genome shotgun sequence.
ACCESSION   DAOSIF010000259 DAOSIF010000000
VERSION     DAOSIF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18426_length_4239_cov_3.154398"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..668
                     /locus_tag="PLT35_09105"
     CDS             387..668
                     /locus_tag="PLT35_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29425.1"
                     /translation="MARGLAAISDRAWDNSGVLVGLVACGTIGYQVVREWRDPAPSSV
                     SLWFVVGFFFVYSFWCLYGVRFGRRGIWLPNGVAAILQVIFAAAILMKR"
     gene            complement(996..1082)
                     /locus_tag="PLT35_09110"
     tRNA            complement(996..1082)
                     /locus_tag="PLT35_09110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1046..1048),aa:Leu,seq:cag)
     gene            complement(1298..2452)
                     /locus_tag="PLT35_09115"
     CDS             complement(1298..2452)
                     /locus_tag="PLT35_09115"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOG29426.1"
                     /translation="MTRCDVWLGAALAACLACPPALAQQPAPGVLGGDLGGLARLSRA
                     KTRSISPENFTGEKGKAGMATTGTGERAARDLGQGWKISPSVRIKAGETFTLGEIAGP
                     GSIQHIWMTPTGNWRYSILRMYWDGEAQPSVEVPVGDFFATGWGAFAPLSSLAVCVNP
                     GSAFNSYWEMPFRKSAKLTMTNIDAADMTLYYQIDYALADVPADAAYFHAQFRRVNPL
                     PYKGVYTILDGVKGWGHYVGTYLAWGVNNSGWWGEGEIKFYMDGDTTFPTICGTGTED
                     YFCGSYNFENKETKQYQVFTTPYAGLSQVIKPDGLYASQQRFGMYRWHLRDPIRFESD
                     LRVTIQALGWRGDGRYLPLQDDIASVAYWYQTEPHAPFPALPERDQLEVR"
     gene            2600..>4239
                     /locus_tag="PLT35_09120"
     CDS             2600..>4239
                     /locus_tag="PLT35_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29427.1"
                     /translation="MPRRICLVASGDLRLSANRVCWPAQAALEAALERAFARFDCAIV
                     RGHPYDPEAQHGFLDSQIRGIEAFRQIDPDMPIVVAEAVWQYTSHVLPGLTKHRGPIL
                     TAANWSGEWPGLVGMLNLNGSLTKAGIRYSTLWSETFEDEFARRGLEQWLTEGRIDHD
                     TSHARPFDASALPDDLRRAAAHGAGLGRQLKARQAVMGVFDEGCMGMYNAIIPDHLLH
                     AAGIFKERLSQSALYAAMREVSDETASRHYEWLAERGMTFDFGPDPAADLTRDQVLEG
                     LKMYDAAVRFAATFGCSAIGIQYQQGLKDVCVASDLAEGLLNCSDRPPVVGADGLVLF
                     DGQPVPHFNEVDECAGLDAILTNRVCTVAGLDPSTTLHDVRWGADFDGHGISEFVWVF
                     EISGAVPPSHLIGGYKGAVGERQPPMYFPKGGATIKGVSKPGEIVWSRVYVEGDALHM
                     DIGRGAALRLPEEESARRWAQTTPQWPMMHAVLYGVTRDQFMAKHQANHIQVVYAPDA
                     MAARRLLSAKAGMAGALGLRVNLCGALDDEIGAHQAEE"
BASE COUNT          607 a         1485 c         1522 g          625 t
ORIGIN      
        1 cccatcgtat cacccgcgga gggcgagcgg ccggctggcg gcgccgatgg cgcccagcgt
       61 gagcggagat tccgcgctcg gccagtcgcc ctgccgcgcg gcaatccgcc gggcattccc
      121 ctcgcgggcg agcgccttgg cgcgctcggt ccgctcgccg ccctcgcccg cgggccccct
      181 ccatcacgtg cgtgcatcgg ccatgggcgc tccctgcgcg agagctgcga gaatcccggg
      241 cggaaggttc cggaagccgc cgactacaat gtgaggggaa ccgcggtgcg gcgcctctcc
      301 ggccaggggc ggctcgagcc tcgcgctcgc gcgtcgcggt ccgtgggttg gagcatgcgg
      361 ggcggcggcc cgggagagga ttcggcgtgg cacgagggct ggcggcgatt tccgacaggg
      421 cgtgggacaa ctcgggcgta ctggtgggcc tcgtggcctg cggcacgatc ggctaccagg
      481 tggtccgcga gtggcgggac cccgcgccgt cctccgtgtc tctctggttc gtcgtgggct
      541 tcttcttcgt ctacagcttc tggtgcctct acggcgtcag gttcggacgc cgcgggatct
      601 ggctgcccaa cggggtcgcc gcgatcctgc aggtgatctt cgccgcggcg atcctcatga
      661 agcggtgatc accgggccgg gcgacgccgg ctctcgccac cgcctgatgc cctcgcaggc
      721 gcccggggac ggcacgcgcg cagcgcggcc cggccgccgg gtcctgacgt cgggccttcg
      781 gacgcagccg gacgaagcgg ccttcgcggc ttcgcctggc gggcctcggc ggagccggcc
      841 ccttgccggc tcgcccgccc gcagaccgtg cgaaagcggc cgccgtgagg tgatggggat
      901 ccttcgactg gctgcgcgcg cagcagacca ttcgcctcgg tggtccgagg ctaaggggct
      961 tgccgcggac gaaggggcga cgcgactcag tcgagtggtg cggaagagag gactcgaacc
     1021 tccacgacct tgcggccaca agctcctgag gcttgcgcgt ctgccaattc cgccacttcc
     1081 gcactcggat agaccggcag gacccctaag tataccaacg ctccaacgcg ccgcgcaaac
     1141 agcgccggga ggtgggggag gaggaattca gaattcagaa tccaccccct tcccttgtcc
     1201 cccgcctccg tcagacggcc tgccggcccg ccctgagctt gtcgaagggc cagctacggc
     1261 gcggcacgcg aaatcccgag tcccgaatcc ggactcccta cctcacctcc agctggtccc
     1321 gctccggcag cgccgggaac ggtgcgtgcg gctcggtctg gtaccagtag gccaccgacg
     1381 cgatgtcgtc ctgcagcggc aggtagcggc cgtcgccgcg ccagccgagc gcctggatgg
     1441 tcacgcgcag gtcgctctcg aaccggatcg ggtcgcgcag gtgccagcgg tacatgccga
     1501 accgctgctg cgaggcgtag agcccgtccg gcttgatcac ctgggagagg ccggcgtacg
     1561 gcgtcgtgaa gacctggtac tgcttcgtct ccttgttctc gaagttgtac gacccgcaga
     1621 agtagtcctc ggtgccggtg ccgcagatcg tcgggaacgt ggtgtcgccg tccatgtaga
     1681 acttgatctc gccctcgccc caccatcccg agttgttgac gccccaggcc aggtacgtcc
     1741 cgacgtagtg gccccagccc ttcacgccgt cgaggatcgt gtagacgccc ttgtacggca
     1801 ggggattcac gcgccggaac tgggcgtgga agtaggccgc gtcggcgggc acgtccgcga
     1861 gcgcgtagtc gatctggtag tagagcgtca tgtcggcggc gtcgatgttc gtcatggtca
     1921 gcttggccga cttgcggaac ggcatctccc agtagctgtt gaacgcgctg ccgggattga
     1981 cgcagacggc cagcgaggag agcggcgcga acgcgcccca tcccgtggcg aagaagtccc
     2041 cgacgggcac ctcgacggag ggctgcgcct cgccgtccca gtacatccgg aggatcgagt
     2101 accgccagtt gcccgtcggc gtcatccaga tgtgctgaat cgagccgggc ccggcgatct
     2161 cgccgagcgt gaaggtctcg ccggccttga tgcgcacgga cggcgagatc ttccatccct
     2221 gtccgagatc gcgggcggcc cgctcgcccg tgccggtcgt ggccatgccg gccttgccct
     2281 tctcgccggt gaagttttcc gggctgatcg agcgcgtctt cgcgcgggag agccgggcga
     2341 ggccgccaag atcgccgcca aggacgcccg gcgcgggctg ctgcgccagt gccggcggac
     2401 aggcgaggca cgccgcgagg gcggcgccga gccacacatc acaccgcgtc atagtcgcct
     2461 cctgcggggc ggagcatacc acgccgcggc ggcttgacag gcgcaggccc ggagccaaag
     2521 atagggctgc gcgcggagtg cgcgtgccct cgggccccgc gggcacgcgc ggcccgacgc
     2581 gcccccggaa aggatcgcca tgccacgccg gatttgtctc gttgccagcg gcgacctgcg
     2641 gctgtccgcc aaccgcgtct gctggcccgc ccaggccgcc ctggaggcgg cgctcgaacg
     2701 cgccttcgcc cgcttcgact gcgccatcgt ccgcggccac ccgtacgacc cggaggcgca
     2761 gcacgggttc ctcgacagcc agatccgcgg catcgaggcg ttccgccaga tcgaccccga
     2821 tatgccgatc gtcgtcgccg aggcggtctg gcagtacacg agccacgtcc tgcccggcct
     2881 gacgaagcac cgcggcccga tcctcacggc cgccaactgg agcggcgagt ggccgggcct
     2941 ggtcggcatg ctgaacctga acggctcgct gaccaaggcc ggcatccgct acagcaccct
     3001 gtggagcgag acgttcgagg acgagttcgc gcggcgcggc ctggagcagt ggctgacgga
     3061 gggccgcatc gatcacgaca cgagccacgc gcgccccttc gacgcgtcgg ccctgccgga
     3121 cgacctgcgg cgggcggcgg cccacggcgc gggcctcggc cgccagctga aggcgcggca
     3181 ggccgtcatg ggcgtcttcg acgagggctg catggggatg tacaacgcga tcatccccga
     3241 ccacctgctg cacgccgcgg gcatcttcaa ggagcgcctg agccagtccg cgctgtacgc
     3301 ggcgatgcgg gaggtgagcg acgagaccgc gtcgcgccac tacgagtggc tcgccgagcg
     3361 cggcatgacg ttcgacttcg ggccggaccc ggccgcggac ctcacccgcg accaggtgct
     3421 cgagggcctc aagatgtacg acgccgcggt ccggttcgcc gccaccttcg ggtgctcggc
     3481 catcggcatc cagtaccagc aggggctgaa ggacgtgtgc gtcgcctccg acctcgccga
     3541 gggcctgctg aactgctccg atcggccgcc ggttgtcggc gcggacggct tggtgctctt
     3601 cgacggccag ccggtcccgc acttcaacga ggtggacgag tgcgcgggcc tcgacgccat
     3661 cctcacgaac cgggtctgta ctgttgcggg gcttgacccg tcgacgacgc tgcacgacgt
     3721 gcggtggggg gccgacttcg acggccacgg gatcagcgag ttcgtgtggg tgttcgagat
     3781 ctcgggcgcc gtgcccccga gccacctgat cggcggctac aagggggcgg tcggcgagcg
     3841 gcagccgccg atgtacttcc cgaagggcgg cgccacgatc aagggggtca gcaagccggg
     3901 cgagatcgtc tggagccgcg tctacgtgga aggggacgcg ctgcacatgg acatcggccg
     3961 cggggccgcg ctgcgcctgc ccgaggagga gtcggcccgg cgctgggcgc agacgacgcc
     4021 gcagtggccg atgatgcacg cggtgctcta cggcgtgacg cgcgaccagt tcatggcgaa
     4081 gcaccaggcg aaccacatcc aggtggtgta cgccccggat gcgatggcgg cccgccggct
     4141 tctgtcggcg aaggccggca tggccggcgc cctgggcctg cgcgtgaacc tctgcggcgc
     4201 gttggacgat gagatcggcg cgcaccaggc cgaggagtt
//
LOCUS       DAOSIF010000260         4234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18459_length_4234_cov_3.542474, whole genome shotgun sequence.
ACCESSION   DAOSIF010000260 DAOSIF010000000
VERSION     DAOSIF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18459_length_4234_cov_3.542474"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1381)
                     /locus_tag="PLT35_09125"
     CDS             complement(92..1381)
                     /locus_tag="PLT35_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOG29428.1"
                     /translation="MGMTVAQKILARASGLPAVTVGDVVEPAVDLAMSHENTALVINQ
                     FLEVFAGTALEPRVWDPSRIAVIFDHRVPAESAKTATNQRKVREFVAAQGIARFHDVR
                     GDSGGICHQVLAEEGYDRPGAVIVGADSHTTSHGALGALAFGIGATEMASVWALGRAV
                     NVQVPASIKVALHGRFGPAVSPKDAILHLVGLLTAEGANFKVIEFHGDAVAAMPTSGR
                     LVLCNMSVEAGATSGIVPADAETVRYLREEAGVTDPLDLVQPDPDAEYDRVVDIDVSA
                     LGPQIACPHTVDNVKPVGAVAGTKVHQIVIGSCTNGRLDDLETAAARLRGRKVASGVR
                     MLVFPASWRIYKEAMRRGYLADLVEAGAVIMNPGCGCCLGVHQGALGDGEVALSTTNR
                     NFKGRMGNASGEVYLCSPAVAAASALAGVITDPRQEG"
     gene            complement(1385..2578)
                     /locus_tag="PLT35_09130"
     CDS             complement(1385..2578)
                     /locus_tag="PLT35_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOG29429.1"
                     /translation="MTLLDGIRVLDLTNVLAGPFATMHLALAGADVIKIENPNGGDLA
                     RKLGNVPALNQELMGTSFLAQNANKRSLTLNLKADEGRRIFLALAATADVVVENFRPG
                     VMARLGLGYETLTAANPRLVYCAISGFGQTGPDAHKPAYDQIIQGLSGVMDVNGDTRL
                     HPLRCGFPVCDTVGGLNAAFAIMGALFHRERTGEGQFIDVAMLDSIMPLMGWVAANLL
                     IGGVPPVPMGNDNFTAAPSGVFTTGDGFINIAANEQAQWESLCDVLGVPELKSDERFA
                     RRDVRKANRALLTPLLEAKLAGRPTAHWVEALNRAGVPSGEILDLRAALAQPQIAHRG
                     TIRTVREPGIGDLKLFNLTARFEKTPGSVDAPPPRLSADTDAILGELGYTADAVADLR
                     QKGVV"
     gene            complement(2639..3565)
                     /locus_tag="PLT35_09135"
     CDS             complement(2639..3565)
                     /locus_tag="PLT35_09135"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOG29430.1"
                     /translation="MAVVTIHEVGLRDGLQIEEQVVPSERKLDWIARLARAGVPVIQV
                     GSFVHPQRVPQMADTDSLFRRLADLPRVPGVAYSALVLNERGLERGLACGVEYFCMGA
                     SASDTHSRKNTGMAASEAADRITAMGRRAAEAGRRVQVSVQSAFGCGFEGRVPAARVL
                     DLVRRFVDAGLRSVSLADTAGHADPAQVESLVASVRAIDDGLALTCHFHDTYGLGLAN
                     AYAALRAGVTTFESAFGGLGGCPFTAVAAGNVCTEDLVHMFQRMGLCGGVDAEAVAEV
                     ARDAAAFFGRALPGVVHRTGPIPERGTAEGAH"
     gene            complement(3568..>4234)
                     /locus_tag="PLT35_09140"
     CDS             complement(3568..>4234)
                     /locus_tag="PLT35_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG29431.1"
                     /translation="RLSETLAALAPAFAPYAALAPDQFAVSCKINTKRGSERIIDAAF
                     AYARAHGRRKVTVVHKANVVRATDGLFLETAREVAARYPDIAWDEANVDAITMWLLKN
                     PLNYDVLVAPNLYGDIVSDLCAQMVGGLGFACSGNIGDTLAVFEPTHGSAPKYAGQHK
                     VNPIATILAAKMMLEWLGEAHLAARVEAAVAAVIRQGQVRTYDMGGSNTTLEMAEAVA
                     REL"
BASE COUNT          585 a         1496 c         1543 g          610 t
ORIGIN      
        1 gacgggagga ccgtcgccat gaaccgtccc gggtagatcg tgtcggtcga cacgtcgtcg
       61 cccaccacgt agaccacctt cgccatggtg ctcatccctc ctgccgcggg tccgtgatga
      121 cgccggcgag cgcgctggcc gccgcaacgg ccggagagca gaggtagacc tcgcccgagg
      181 cgttgcccat ccggcccttg aagttccggt tcgtcgtcga gagcgccact tcgccgtcgc
      241 cgagcgcgcc ctggtggacg cccaggcagc agccgcaccc cgggttcatg atgacggcgc
      301 cggcctcgac gaggtccgcg agatagccgc gccgcatggc ctccttgtag attctccagg
      361 acgcggggaa gacgagcatc cgcacgccgc tcgccacttt ccggcccctc aaccgggccg
      421 cggccgtctc gagatcgtcg aggcgcccgt tggtgcagga gccgatgacg atctgatgga
      481 ccttcgtgcc ggccacggcg ccgaccggct tgacgttgtc caccgtgtgc gggcacgcga
      541 tctgcggccc gagggccgac acgtcgatgt cgacgacgcg gtcgtactca gcgtccgggt
      601 ccggctgcac cagatcgagc gggtccgtga cgcccgcttc ctcgcgcagg tagcggaccg
      661 tctcggcgtc ggcggggacg atgcccgacg tggcgccggc ctccacgctc atgttgcaca
      721 gcacgaggcg gcccgaggtc ggcatggcgg cgacggcgtc gccgtggaac tcgatcacct
      781 tgaagttcgc gccttccgcg gtgaggaggc cgacgaggtg gaggatggcg tccttcggcg
      841 agaccgccgg cccgaaccgc ccgtgcaggg ccaccttgat cgacgccggg acctggacgt
      901 tgacggcgcg gccgagcgcc cacaccgagg ccatctccgt ggcgccgatg ccgaaggcga
      961 gggcgccgag ggcgccgtgg ctcgtggtgt ggctgtcggc gccgacgatc acggcgcccg
     1021 gccggtcgta gccctcctcg gccaggacct ggtggcagat gccgcccgag tcgccgcgca
     1081 cgtcgtggaa cctggcgatg ccctgcgccg cgacgaactc gcggaccttt cgctggttcg
     1141 tcgccgtctt ggccgactcg gcggggaccc ggtgatcgaa gatgacggcg attctcgacg
     1201 ggtcccagac gcgcggctcg agggccgtgc ccgcgaacac ctcgaggaac tggttgatga
     1261 cgagcgcggt gttctcgtgc gacatcgcga ggtcgacggc cggctccacc acgtcgccga
     1321 cggtgacggc cggcaggccg gaggcccgcg ccaggatctt ctgcgcgacg gtcatgccca
     1381 tgggtcagac gactcccttc tggcggagat cggcgacggc gtcggcggtg tagccgagct
     1441 cgccgaggat cgcgtcggtg tcggccgaga ggcgcggcgg cggcgcatcc accgaccccg
     1501 gggtcttctc gaaccgcgcc gtgaggttga agagcttgag atcgccgatg cccggctcgc
     1561 gcaccgtccg gatcgtgccg cggtgcgcga tctgcggctg cgccagcgcc gcgcgcaggt
     1621 cgaggatctc gcccgacggg acgcccgcgc ggttcagcgc ctcgacccag tgggcggtcg
     1681 ggcgcccggc cagcttcgct tcgaggagcg gcgtgaggag cgcgcggttg gccttgcgca
     1741 cgtcccggcg cgcgaaccgc tcgtcgctct tcagttcggg gacgccgagc acgtcgcaga
     1801 gcgactccca ctgcgcctgc tcgttggccg cgatgttgat gaagccgtcc ccggtggtga
     1861 acacgcccga aggcgccgcg gtgaagttgt cgttgcccat cggcaccggc ggcacgccgc
     1921 cgatgagcag gttggccgcg acccagccca tgagcggcat gatcgagtcg agcatggcga
     1981 cgtcgatgaa ctggccttcg cccgtccgct cgcggtggaa gagggcgccc atgatggcga
     2041 aggcggcgtt gaggccgccc accgtgtcgc acaccgggaa accgcagcgg agcgggtgca
     2101 ggcgcgtgtc gccgttgacg tccatgacgc cgctgaggcc ctggatgatc tgatcgtacg
     2161 ccggcttgtg ggcgtcgggg cccgtctggc cgaagccgga gatggcgcag tagacgagcc
     2221 gcgggttcgc cgccgtcagc gtctcgtacc cgagcccgag ccgcgccatg acgccgggac
     2281 ggaagttctc caccacgacg tcggccgtcg ccgcgagggc gaggaagatg cggcggccct
     2341 cgtccgcctt cagattgagc gtcagcgagc gcttgttggc gttctgcgcg aggaagctcg
     2401 tgcccatcag ctcctggttg agcgccggca cgttgccgag cttgcgcgcc aggtcgccgc
     2461 cgttcgggtt ctcgatcttg atgacgtccg ccccggccag cgccaggtgc atggtggcga
     2521 aggggccggc aaggacgttc gtcaggtcca gcacccgaat cccgtcgagc agtgtcatgg
     2581 ctgcagtcac ctcggtccgc gaaggggccg gccggcgtgg cgcgccgcgg gccggccgct
     2641 agtgagcgcc ctccgccgtt ccccgctcgg ggatggggcc cgtgcggtgc acgacgcccg
     2701 gcagcgcgcg gccgaagaag gcggccgcgt cgcgggcgac ctcggcgacc gcctcggcgt
     2761 ccacgccgcc gcacaggccc atgcgctgga acatgtgcac caggtcctcc gtgcacacgt
     2821 tgcccgccgc aacggcggtg aacgggcagc cgccgaggcc gccaaacgcc gactcgaacg
     2881 tcgtgacgcc ggcgcgcaag gcggcgtacg cgttcgccag gccgaggccg taggtgtcgt
     2941 ggaaatggca cgtgagcgcc aggccgtcgt cgatcgcgcg gaccgacgcc acgagcgatt
     3001 cgacctgcgc cgggtccgcg tggccggccg tgtcggccag gctcaccgag cggagaccgg
     3061 cgtcgacgaa ccgcctgacc aggtccagca cgcgggcggc cggcacgcgc ccctcgaagc
     3121 cgcagccgaa ggccgactgc accgacacct ggacgcgccg gcccgcctcg gccgcccggc
     3181 ggcccatggc ggtgatccgg tcggcggcct cggaggcggc catgcccgtg ttcttccggc
     3241 tgtgcgtgtc gctggccgac gcgcccatgc agaagtactc gacgccgcac gcaaggccgc
     3301 gctcgaggcc gcgctcgttc agcaccaggg ccgagtaggc gacgcccggg acgcggggca
     3361 gatcggcgag gcggcggaag aggctgtcgg tgtccgccat ctgggggacc cgctgcggat
     3421 gcacgaacga tcccacctgg atgaccggca cgcccgcgcg cgccaggcgc gcgatccagt
     3481 cgagcttgcg ctcgctcggg acgacctgtt cctcgatctg cagaccgtcg cgcagcccga
     3541 cttcgtggat ggtgacgacc gccatcgcta gagctcccgg gccacggcct ccgccatttc
     3601 cagcgtggtg ttcgagccgc ccatgtcgta ggtgcggacc tggccctggc ggatgaccgc
     3661 ggccacggcc gcctcgacgc gggcggccag gtgggcctcc ccgagccact cgagcatcat
     3721 ctttgccgcg agaatcgtcg cgatcgggtt gaccttgtgc tggcccgcgt acttcggcgc
     3781 cgagccgtgc gtcggctcga agacggccag ggtgtcgccg atgttgccgg agcaggcgaa
     3841 gccgagcccg ccgaccattt gcgcgcacag gtcggagacg atgtcgccgt acaggttcgg
     3901 cgccacgagg acgtcgtagt tgagcgggtt cttgaggagc cacatcgtga tggcgtccac
     3961 gttcgcctcg tcccacgcga tgtccggata gcgggccgcc acttcccgcg ccgtctccag
     4021 gaacaggccg tcggtcgcgc ggaccacgtt cgccttgtgg accaccgtca ccttcctgcg
     4081 gccgtgcgcg cgcgcgtagg cgaaggccgc gtcgatgatc cgctcggacc cgcgcttcgt
     4141 gttgatcttg caggacacgg cgaactggtc gggggcgagc gccgcgtacg gcgcaaaggc
     4201 cggcgcgagg gccgcgagcg tctccgagag ccgc
//
LOCUS       DAOSIF010000261         4221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18523_length_4221_cov_4.806049, whole genome shotgun sequence.
ACCESSION   DAOSIF010000261 DAOSIF010000000
VERSION     DAOSIF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18523_length_4221_cov_4.806049"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLT35_09145"
     CDS             complement(<1..442)
                     /locus_tag="PLT35_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG29432.1"
                     /translation="MAEHRIREHPILPIPDARPVTFSWNGKPCTGHEGETIASALFAN
                     GVRVFGHHPKDGAPQGIFCANGQCAQCTVVADGLAVKSCMVPVRPGMEVRPLDGKPAL
                     PEVHHAPDLRDVPVVDTGCLIIGGGPAGLTAAIELGRAGVRTLVV"
     gene            complement(459..1712)
                     /locus_tag="PLT35_09150"
     CDS             complement(459..1712)
                     /locus_tag="PLT35_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG29433.1"
                     /translation="MPTIAALHDRIDQAAAAVLADVVAWRRDLHAHPELGYEEVRTSA
                     FLAERLRAMGYTVRTGVARTGVVGLLTGGRPGPVVALRADMDALPVAEETGLPFASAA
                     RGVYAGKEVGLMHACGHDFHMAMLLGAGHVLASMKADLAGAVKIIFQPAEEGIAGEPG
                     GAEAMIREGVLENPTVDAMFGLHVGITPEEAGSITFRPRGLMAAGNTFSIVVKGRQTH
                     GAIPWAGTDSIVVASQIVLGLQTVVSRQIDITEAPAVVTVGTIEGGTRHNIVPGEVRM
                     TGTIRTFDPGMRAEILERVRRTAAGIAESAGAAADVRIDEGYPVTWNDPALTAQMTPS
                     LRRVAAAAFNPNVKPTTTSEDFSYYGQKVPALFFFLGVAPKGSDPAAWAANHSPKFNP
                     DESALITGVRALASLAVDYLSAASK"
     gene            complement(1966..2967)
                     /locus_tag="PLT35_09155"
     CDS             complement(1966..2967)
                     /locus_tag="PLT35_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG29434.1"
                     /translation="MPTRLPRLPGAVRGRDFIETRDWTVPEIELLLETSDRLKRAFRA
                     GRPTRYLPDKTVFLFFFDKSTRTRNSFEAGATQLGAHAHFIAAETSQAAHGESPRDMG
                     IILSSYGHAIAIRHDLVPGEGNRYMREVAEHATVPVFNMQCDVDHPFQTLADLMTIRE
                     EFGRDLRGRRIAVTWAYAPSYAKPMSVPQGLITLLPRFGLDVALAHPPEWKLMDAPLA
                     FAREQAKKHGTSFEVTGDMDEACRGADIVYAKSWGIESLFHKPEEALALSARYKSWIC
                     DERRMRLASKDAIYMHCLPADRNHEVTDAVIDGPQSRVFREAENRLHTCKAVMALTM"
     gene            3088..3762
                     /locus_tag="PLT35_09160"
     CDS             3088..3762
                     /locus_tag="PLT35_09160"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOG29435.1"
                     /translation="MTKHGTGLAALALALAAGWLWPTPAAAQVLTRLENAVTVFDEAT
                     SGGDASIPEDLLKKAECVVIVPGLKKGAIGIGFKYGRGFVSCRSEDGWSPPAGIRMEG
                     GSYGFQIGGAEVDVILTVMNKRGVDRLLSSKFTLGADASVAAGPVGRSASAQTDAMMT
                     AEILSWSRSRGIFAGIALGGSTLRGDDDANKDLYGKKVRVRDIVTGGLPAPAAASALL
                     ERLAKY"
     gene            3932..>4221
                     /locus_tag="PLT35_09165"
     CDS             3932..>4221
                     /locus_tag="PLT35_09165"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="catalyzes the formation of propanoyl phosphate from
                     propanoate and ATP; TdcD also has acetate kinase
                     activities and functions in anaerobic threonine
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate/acetate kinase"
                     /protein_id="HOG29436.1"
                     /translation="MDVLVLNCGSSSVKFAVIDPVSSQEEVSGIAQRLGAPEASLDWK
                     RHGKKDTRPTPRAGHEEALRAIGNLLNELGLTDELIGVGHRVVHGGSKFSGS"
BASE COUNT          598 a         1480 c         1498 g          645 t
ORIGIN      
        1 ccacgacgag cgtccgcacg ccagcccgcc cgagctcgat ggccgccgtc aggccggccg
       61 gcccgccgcc gatgatcagg cacccggtgt cgacgacggg gacgtcccgc agatcgggcg
      121 cgtggtgcac ctccggcagg gccggcttgc cgtcgagcgg ccggacctcc atgcccggcc
      181 ggaccggaac catgcacgac ttgacggcca gcccgtcggc gaccacggtg cactgcgcgc
      241 actggccgtt ggcgcagaag atgccctgcg gcgcgccgtc cttcgggtga tggccgaaca
      301 cccgcacgcc gttggcgaag agggccgacg cgattgtctc accttcgtgt ccggtgcacg
      361 gcttcccgtt ccaggagaac gtgacgggcc tggcgtcggg aatcgggagg atcgggtgct
      421 cgcggatgcg gtgctcggcc acgggtgtca gctctccttc acttcgacgc ggcgctgagg
      481 tagtcgacgg cgagactggc gagggcgcgc acgccggtga tgagcgccga ctcgtccggg
      541 ttgaacttcg gcgagtggtt ggccgcccac gcggccgggt ccgagccctt cggcgccacg
      601 ccgaggaaga agaacagcgc cggcaccttc tgcccgtagt acgagaagtc ctcggaggtc
      661 gtcgtcggtt tcacgttggg gttgaaggcg gccgccgcga cccgacggag cgacggcgtc
      721 atctgggcgg tcagcgcggg atcgttccac gtgaccgggt agccctcgtc gatccggacg
      781 tcagccgccg ccccggccga ttcggcgatt cccgccgccg tgcgccggac gcgctccagg
      841 atctcggcgc gcatgccggg gtcgaaggtc ctgatggtcc cggtcattcg cacctcgccg
      901 ggaacgatat tgtgccgggt gccgccctcg atggtgccga cggtcacgac cgccggcgcc
      961 tcggtgatgt cgatctgccg gctcaccacc gtctgcaaac cgagcacgat ctgcgatgcg
     1021 acgacgatcg aatcggtgcc ggcccaggga atggcgccgt gcgtttgccg gcccttcacg
     1081 acgatgctga aggtgttgcc ggcggccatc aggccccgcg gccggaacgt gatcgagccg
     1141 gcttcctcgg gcgtgatgcc gacgtgcaaa ccgaacatgg catcgaccgt cgggttctcg
     1201 agcacgccct cccggatcat cgcctccgcg ccgccgggct ctccggcgat accctcctcc
     1261 gccggctgga agatgatctt cacggcgccg gcgaggtccg ccttcatcga ggccagcacg
     1321 tgcccggcgc cgagaagcat cgccatgtgg aagtcgtggc cgcaggcgtg catgaggccg
     1381 acctccttgc ccgcgtacac gccgcgcgct gcggaggcaa acggcaggcc ggtttcctcg
     1441 gcgaccggca gcgcgtccat gtccgcccgg agcgccacca cgggccctgg ccgcccgcct
     1501 gtgaggagcc cgaccacgcc ggttctcgca acgccggtcc gcacggtgta gcccatggcg
     1561 cgaagccgct cggcgagaaa cgccgacgtg cgcacctcct cgtatcccag ctcgggatgc
     1621 gcgtggaggt ccctgcgcca ggcgacgacg tcggcgagca ccgccgcggc ggcctggtcg
     1681 atccggtcgt gaagcgcggc gatcgtcggc atcggtcgtc cttcctgggc tcgctccggc
     1741 cgcccgggag cgccctctgc ggccgccgcc accgcgatcg ccaccgccga aaccagcacg
     1801 attcccgatc gcgtcgcagc cgatcgtccc acgtccctgc tcccgcctgt ccggcccggg
     1861 gcggcgcccc gaaccccgag tcccgaactc cgagtcccga gtcccgcgtc ccgagtcccg
     1921 ggtcccgggt cccgagcccc gggtcccgag ccccgggttc cttccctaca tcgtcagcgc
     1981 catgaccgcc ttgcacgtgt ggagccggtt ctcggcctcc cggaagaccc tcgactgcgg
     2041 cccgtcgatc acggcgtcgg tcacttcgtg gttgcggtcg gcgggcaggc agtgcatgta
     2101 gatcgcgtcc ttggacgcga gccgcatgcg ccgctcgtcg cagatccagc tcttgtaccg
     2161 ggccgagagc gcaagcgcct cctccggctt gtggaagagc gactcgatgc cccacgactt
     2221 ggcgtagacg atgtccgcgc cgcggcaggc ctcgtccatg tcaccggtga cctcgaacga
     2281 ggtgccatgc ttcttcgcct gctcccgcgc gaaggccagc ggagcgtcca tcagcttcca
     2341 ctccggggga tgggccagcg ccacgtccag gccgaagcgc ggcagcagcg tgatcaaccc
     2401 ctgagggacg gacatcggct tggcgtagct cggcgcgtag gcccacgtca cggcgatgcg
     2461 gcggcccctg aggtcccgcc cgaactcctc gcggatggtc atcaggtccg cgagcgtctg
     2521 gaacgggtgg tcgacgtcgc attgcatgtt gaagacgggc acggtggcgt gctcggcgac
     2581 ctcgcgcatg tagcggttgc cctcgcccgg cacgaggtcg tgccggatgg cgatggcgtg
     2641 gccgtagctc gacaggatga tgcccatgtc cctcggcgac tcgccgtggg ccgcctgcga
     2701 ggtctcggcc gcgatgaaat gcgcgtgggc gccgagctgc gtggcgccgg cctcgaacga
     2761 gttccgcgtg cgggtggact tgtcgaagaa gaacaggaag accgtcttgt ccggcaggta
     2821 ccgcgtgggc cgtccggcgc ggaacgcccg tttcaggcgg tccgacgtct cgagcagcag
     2881 ctcgatctcg gggacggtcc agtctcgcgt ctcgatgaag tccctgcctc ggaccgcgcc
     2941 gggcagccgg gggagtcggg tgggcatacc ggcgaagtct acgtcagcgg cccctgcctc
     3001 cgctacggcc gcgtggcggc ctccggttgc cgggcacgcg gcgggcgatc tacgctggtg
     3061 tggcacggca ggcgtgcgga ggttgacatg acgaaacacg gaacgggact cgcggccctc
     3121 gccctcgcgc tggcggccgg gtggctctgg ccgacgccgg cggcggccca ggtgctgacc
     3181 cgcctggaga acgccgtgac ggtgttcgac gaggccacca gcggcggcga cgcgtcgatc
     3241 cccgaggatc tcctgaagaa ggccgagtgc gtcgtcatcg tgcccgggct gaaaaagggt
     3301 gccatcggca tcggcttcaa gtacgggcgg gggttcgtct cctgccggag cgaagacggc
     3361 tggtcgccgc ccgcggggat ccggatggag ggcgggagct acgggtttca aatcggcggc
     3421 gccgaggtcg atgtcatcct gacggtgatg aacaagcgcg gcgtggaccg gctgctttcg
     3481 agcaagttca ccctcggagc ggatgcctcg gtggcagcgg ggcccgtcgg ccgatccgcg
     3541 tccgctcaga ccgacgcgat gatgacggcg gagatcctgt cgtggtcgag gtcgcgcggg
     3601 atcttcgcgg gcatcgcgct cggcgggtcg acgctccgcg gcgacgatga tgcgaacaag
     3661 gatctctacg gcaagaaggt ccgcgtccgc gacatcgtca ccggggggct ccccgcgccc
     3721 gcggccgcgt cggcgctcct ggagcggctg gcgaagtact gattgcctct cagcggcgcc
     3781 gccgggcccc ggacttggcc cgcggcgccg gaacgggtag gatattgcgg tttgccggcc
     3841 ggagtgtggc cggtcgtgcg gtcttcagca accccgttcg gtggcccacc gcccctgatg
     3901 ccattccgga cgtctcaggc aggtagcgcc catggacgta ctcgtactca actgcggttc
     3961 ctcgtccgtg aagttcgccg tcatcgatcc cgtatcgtcc caagaggagg tctccggaat
     4021 cgcgcagcgc ctcggcgccc ccgaagccag cctcgactgg aagcgccacg gcaagaaaga
     4081 caccaggccc actcccaggg ccgggcatga agaggcgctc cgggcgatcg ggaacctgct
     4141 gaacgaactg gggctcaccg acgagctcat cggcgtcggg caccgcgtcg tgcacggggg
     4201 ctcgaagttc tcggggtcga t
//
LOCUS       DAOSIF010000262         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18662_length_4200_cov_3.191556, whole genome shotgun sequence.
ACCESSION   DAOSIF010000262 DAOSIF010000000
VERSION     DAOSIF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18662_length_4200_cov_3.191556"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..2076
                     /locus_tag="PLT35_09170"
     CDS             187..2076
                     /locus_tag="PLT35_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOG29437.1"
                     /translation="MAVRIVHPVPSDIDIAQAATLLPIADVAASLGLAPDDLDFYGKH
                     KAKIHLDVLERLQDAPMGHYVDVTAITPTPLGEGKTTTTVGLCQALGAHLGKKVVTVV
                     RQPSLGPTFGIKGGAAGGGYSQIVPMEDFNLHLTGDIHAVTASHDLMAAAIDARILHE
                     STQDDDKLFNAFCPADKQGRRRFSPTMLRRLRKLGIDKTDPADLTPAERRRMFRLDID
                     PATITWNRVIDISDRFLRAVQIGRGPDEKGMVRETGFDISVASEIMAILALTTGLADM
                     RERLGRTVIGTSRAGEPVTAEDLGVAGALTVLMKDAIMPNLMQTLENTPAIVHAGPFA
                     NIAHGNSSVIADQIALRLVGRDGYVVTESGFGADCGMEKFMNIKCRASGLAPSAVVIV
                     ATVRALKMHGGGPRVVAGRPLAPEYTEENLGLLAAGLPNLAAHIRNARRFGVPAVVAV
                     NRFTADTDAEIELIRRTAIDAGAEDAIPCSNWTDGGAGAVALAKGVIAACEKPSEFRF
                     LYPLEWSIKRKIETIATEIYGADGVDYAPEAEAKVELYTRLGFGTLPICMAKTHLSLS
                     HDPALKGVPTGFRIPVRDIRASVGAGFLYPLCGDMRTMPGLPTRPVFFDVDLDLETGR
                     VRGLF"
     gene            complement(2073..>4200)
                     /locus_tag="PLT35_09175"
     CDS             complement(2073..>4200)
                     /locus_tag="PLT35_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOG29438.1"
                     /translation="TCVAILEVVARLVPSPPCRSLLVLGFDDVYLAADAVEDVTAAGP
                     IGLEGIDELLVRHSRKKNLNPKGIALLPEGQGWLFVEFGADTAADAEGQAERLMERMR
                     QRAGAPSMRLFRDPAEAQLVWAVRESALGATSFVPGEAKNWEGWEDAAVPPGRLGEYL
                     RALRRKMSDFGYTGSLYGHFGQGCVHTRINFDLGTPDGIATYRRFVEEAADLVVSLGG
                     SISGEHGDGQSRGELLPKMYSGELMQALREFKALWDPDNKMNPGKLVSPYRLDEHLRV
                     PQYRPPEVKTHFHYPVEGSFADAAMRCVGVGKCRKTDGGAMCPSYMATGDERHATRGR
                     ARLLFEMLQGDIVTDGFRSEAVKESLDLCLSCKSCKSECPTGVDLAAYKAEFLAHYYE
                     GRRRPLRAYAFGFINYWGALAERAPWLANAFTQAPPFRGLVKWALDMPAERRVPAFAR
                     RSFRRAFTAAQRFAPPASRVPAPARGRVILWPDCSNNYFHPEVSLAAARVLTAAGFQV
                     EIPAKRLCCGRPLYDHGLLSAAKRRLAEVLGALRDDIEAGTPIVGLEPSCISVFRDEL
                     LRFFPDDPLAQTLSRQAWLLTEFLVKTDSIPEVRLSGRAVVHPHCHERAALCLDDDIE
                     IMKRTGLDFTVLDSGCCGMAGAFGFEKSHYGVSMAIGERVLLPAVRRAPPGTYIVTNG
                     FSCREQIEQTTGRRVWHMAELLAEGL"
BASE COUNT          647 a         1520 c         1439 g          594 t
ORIGIN      
        1 ccttgtggcc ttcgccatct gcggcccggg gatttgaacc gtcggctggc cggcagccac
       61 ggccgccggg cagttatgcc atacaatgtc ccccggcgtt tcgcacggcg cttcgcccag
      121 cccccgaccg ggaatcgagc ccggcccgaa agccgccgca agccgacccg tccaggagga
      181 tgtgccatgg ccgtcaggat cgtccatccc gttcccagcg acattgacat cgcccaggca
      241 gcgacgctgc tgcccattgc cgacgtggcg gcgagcctcg gcctggcgcc cgacgacctc
      301 gacttctacg gcaagcacaa ggcgaaaatc catctcgacg tcctcgagcg gctgcaggac
      361 gcgccgatgg gccactacgt cgacgtgacg gccattacgc ccaccccgct cggcgaagga
      421 aagacgacga cgactgtcgg gttgtgccag gcgctcggtg cgcacctcgg caagaaggtg
      481 gtgaccgtcg tgcgccagcc gagcctgggc ccgacgttcg ggatcaaggg cggcgccgcc
      541 ggcggcggct actcgcagat cgtgccgatg gaggacttca acctccacct gacgggcgac
      601 attcacgcgg tgacggcctc ccacgatctg atggccgcgg ccatcgacgc ccgcatcctc
      661 cacgagtcga cccaggacga cgacaagctc ttcaacgcct tctgccccgc cgacaagcag
      721 ggccggcggc gcttctcgcc gaccatgctg cgccgcctca ggaagctcgg tatcgacaag
      781 acggaccccg ccgacctcac gcccgccgag cgccggcgga tgttccgcct cgacatcgat
      841 cccgcgacga tcacgtggaa ccgggtgatc gacatcagcg accggttcct gcgcgcggtg
      901 cagatcggcc gcgggcccga cgagaagggg atggtccgcg agaccgggtt cgacatctcg
      961 gtcgcgtccg agatcatggc catcctcgcc ctgaccacgg gcctggccga catgcgcgag
     1021 cggctggggc gcaccgtcat cggcacgagc cgcgcgggcg agcctgtgac cgccgaggat
     1081 ctcggcgtgg cgggcgcgct gaccgtcctg atgaaggacg cgatcatgcc gaacctcatg
     1141 cagacgctgg agaacacgcc cgccatcgtt cacgcggggc ccttcgccaa catcgcccac
     1201 ggcaactcct ccgtcatcgc ggatcagatc gcgctccggc tcgtcggccg cgacggctac
     1261 gtcgtcaccg agtcgggctt cggcgcggac tgcgggatgg agaagttcat gaacatcaag
     1321 tgccgcgcaa gcggcctcgc cccctccgcg gtggtcatcg tcgcgacggt ccgggcgttg
     1381 aagatgcacg gaggcgggcc gagggtcgtg gccggacggc cgctggcccc ggagtacacg
     1441 gaggagaacc tcggcctgct ggccgcgggc ctgcccaacc tggccgcgca catccgcaac
     1501 gcgcggcggt tcggggtccc cgccgtcgtg gccgtcaacc ggttcaccgc ggacaccgac
     1561 gccgagatcg agctcatccg ccggaccgcc atcgacgccg gagccgagga cgcgatcccc
     1621 tgttcgaact ggacggacgg gggcgccggc gcggtcgcgc tggccaaggg cgtcatcgcc
     1681 gcctgcgaga agccgtcgga gttccgcttc ctctatccgc tggagtggag catcaagcgg
     1741 aagattgaga cgatcgccac cgagatctac ggcgccgacg gcgtcgacta cgccccggag
     1801 gcggaggcca aagtcgagct ctacacgcgc ctcggcttcg gcacgctgcc gatctgcatg
     1861 gccaagacgc atctcagtct gagccacgat ccagcgctga agggcgtgcc gacggggttc
     1921 cgcatcccgg tccgcgacat cagggcgagc gtcggggccg gcttcctgta cccgctgtgc
     1981 ggggacatgc ggaccatgcc cggcctgccg acgcggccgg tcttcttcga cgtcgatctc
     2041 gacctcgaga cgggcagggt gcgcgggctg ttctagagcc cttcggccag cagctcggcc
     2101 atgtgccaga cgcgccggcc cgtggtctgc tcgatctgct cgcggcagct gaagccgttc
     2161 gtcacgatgt aggtgccggg cggcgcgcgg cgcacggcgg gcagcagcac gcgctcgccg
     2221 atggccatcg agacgccgta atgggacttc tcgaagccga acgcgcccgc catgccgcag
     2281 cagcccgaat cgaggacggt gaagtcgagc cccgtccgct tcatgatttc gatgtcgtcg
     2341 tccaggcaca gggccgcgcg ctcgtggcag tgcgggtgca cgacggcccg gcccgacagc
     2401 cgcacctccg ggatcgagtc ggtcttcacg aggaactcgg tcaggagcca cgcctgcctg
     2461 ctcagcgtct gcgcgagcgg atcgtcggga aagaaccgca gcagctcgtc gcggaacacc
     2521 gagatgcagc tcggctccag gcccacgatc ggcgtgccgg cctcgatgtc gtcccgcagc
     2581 gcgccaagga cttctgccag gcggcgcttc gccgcgctca gcaggccgtg gtcgtagagc
     2641 gggcggccgc agcacagccg cttggccggg atctccacct ggaagccggc ggccgtcagc
     2701 accctggccg ccgcgagcga gacctcgggg tggaagtagt tgttcgagca atccggccag
     2761 aggatcacgc ggccgcgggc cggcgccggg acgcgggagg cgggaggcgc gaagcgctgc
     2821 gccgcagtga aggcgcgccg gaagctgcgt cgcgcgaagg cgggcacgcg gcgctcggcg
     2881 ggcatgtcga gcgcccactt gaccaggccc ctgaacggcg gagcctgcgt gaaggcgttc
     2941 gcgagccacg gcgctcgctc ggcgagcgcc ccccagtagt tgatgaagcc gaaggcgtac
     3001 gcccggagcg gccggcgccg gccttcgtag tagtgagcga ggaactcggc cttgtaggcg
     3061 gccaggtcca cgcccgtcgg gcactcgctc ttgcagctct tgcacgacag gcagaggtcg
     3121 aggctctctt tgacggcctc gctgcgaaag ccgtccgtaa cgatgtcgcc ctggagcatc
     3181 tcgaagagca gccgcgcgcg gccgcgcgtg gcgtgccgct cgtcgcccgt cgccatgtag
     3241 ctggggcaca tcgcgccgcc gtcggtcttg cggcacttcc ccaccccgac gcagcgcatc
     3301 gcagcgtcgg cgaagctgcc ctccaccggg tagtggaagt gcgtcttcac ctcgggcggg
     3361 cggtactggg gcacgcggag gtgctcgtcc agccggtacg gcgagacgag cttgcccggg
     3421 ttcatcttgt tgtccgggtc ccagagcgcc ttgaactccc gcagggcctg catcagctcg
     3481 ccgctgtaca tcttcggcag cagctccccg cgcgactgcc cgtcgccgtg ctcgccggag
     3541 atcgacccgc ccaggctcac caccaggtcg gcggcctcct ccacgaagcg ccggtaggtg
     3601 gcgatgccgt cgggcgtccc gaggtcgaag ttgatgcgcg tgtgcacgca gccctggccg
     3661 aagtgcccgt agagcgagcc ggtgtacccg aagtccgaca tcttccgccg cagcgcccgc
     3721 aggtactcgc ccagtcgccc gggcggcacg gcggcgtcct cccagccctc ccagttcttc
     3781 gcctcacccg gaacgaacga cgtcgcgccg agggccgact cgcgcacggc ccagacgagc
     3841 tgggcttcgg ccggatcgcg gaagagccgc atcgacgggg cgccggcccg ctgccgcatc
     3901 cgctccatca gccgctcggc ttgcccttcg gcgtccgccg ccgtgtcggc cccgaactcc
     3961 acgaagagcc atccctgccc ctcgggcagc agcgcaatcc ccttcgggtt gaggttcttc
     4021 ttgcgcgagt gccggacgag cagctcgtcg atgccctcca gcccgatcgg gcctgcggcc
     4081 gtcacgtcct cgacggcgtc ggcggccagg tacacatcgt cgaagcccag cacgaggagc
     4141 gaccggcagg gcgggctcgg gaccagcctg gccacaacct cgaggatcgc gacgcacgtg
//
LOCUS       DAOSIF010000263         4191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18704_length_4191_cov_3.535300, whole genome shotgun sequence.
ACCESSION   DAOSIF010000263 DAOSIF010000000
VERSION     DAOSIF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18704_length_4191_cov_3.535300"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /gene="mreD"
                     /locus_tag="PLT35_09180"
     CDS             complement(<1..399)
                     /gene="mreD"
                     /locus_tag="PLT35_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOG29439.1"
                     /translation="MKTALGPAAFLLAAVLLQGVLSGAAGGAFVDLVLVAVVAVGLRH
                     GRVAGLASGAITGLLQDALGGGILGLSGLGKSVAGYLAGVAATQFIVAGAAARGLVFA
                     GGTLVSAAGFIGLSVLLGLRRYDHPLLEAGS"
     gene            complement(396..1256)
                     /gene="mreC"
                     /locus_tag="PLT35_09185"
     CDS             complement(396..1256)
                     /gene="mreC"
                     /locus_tag="PLT35_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOG29440.1"
                     /translation="MARRDLSSRAPLLLAAVIVAHVLLVSAQVGTAGGSSLLRTGIVG
                     LVTRLQEGSWLAVGVLRSIVDGYVALWHVREENARLARDAEALRGALAEARASASGTE
                     GLRALVGVRASVPWTTTGARVVAGSASPDYRSVTIDKGSRDGVRRNMAVIAQGGVVGR
                     VAIAAASASTVQFVVDRSAAVSGRVERTRSEGIALGNGDATLRFEYLSATADVQPDDA
                     VVTAGLDGIYPAGLALGRVVDVRRAGPAYTRVTIKAAVDFSRLEYVLVIVSPRQPDVA
                     PPRAGAEEGG"
     gene            complement(1260..2285)
                     /locus_tag="PLT35_09190"
     CDS             complement(1260..2285)
                     /locus_tag="PLT35_09190"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG29441.1"
                     /translation="MAFRSLFSLFSSDLAIDLGTANTCVYARGRGIVVNEPSIVAINK
                     LNGKVEAVGNAAKDMLGRTPGNIVAIKPMKDGVIADFEITEKMLTHFIKQAHNGAMWV
                     RPRIVIGVPSEITQVEKRAVKESAYRAKASEVYLVEEAMASAIGAGMPITEPTGNMIV
                     DIGGGTSDIAVISLAGIVYSKAVRVAGNEMDESIIQYIKRNHNLLIGERTAEQIKMEI
                     GSAFPLDERLSMEIKGRHLVEGVPKTITITDEEIRDALAETINVIVDAVRVALERTPP
                     ELSADIVDRGIVLSGGGALLRNLDKRLREETGLPLAMAEDPLSSVVLGAGKMLGDFNL
                     LRKISID"
     gene            complement(2311..3384)
                     /locus_tag="PLT35_09195"
     CDS             complement(2311..3384)
                     /locus_tag="PLT35_09195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG29442.1"
                     /translation="MSPTRSPGEAGGQPLDFAERLRRGQPELRRALRRRSVLEEVVRE
                     AYADTDPARIARMLVDRVSAWIPADTWAVLTSDGTGQVRLLHARDHQGPDEPTLLGVA
                     SWVMMHGSEFVSADLRRDGRVQAAGDATVVALPLAGRGRRAAVLVGFDRRPSARRPRF
                     AAGVRPLLESVLGMMALALDHALLLQQAEALSVTDDLTGLHNSRFLRDALHRETKRAV
                     RYSRPLSVLFIDLDGFKSVNDTHGHLCGSRAIVEAATVIRGCARESDIVARYGGDEFV
                     VVLPDTPAEGAVVVARRVRDRLAAFPFLQPLHLDVRLTASVGIATLPDVTLAPEELLR
                     AADEAMYRVKERGKNSYCLAPPR"
     gene            3588..>4191
                     /locus_tag="PLT35_09200"
     CDS             3588..>4191
                     /locus_tag="PLT35_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29443.1"
                     /translation="MDAANAHAATLGLIIGNRGFFPDHLARSGREEMLAALGAAGVEV
                     VALGPEDSKCGAVETPAEARRCADLFRARRDDIDGVIISLPNFGEERALADALRFSTL
                     DVPVLVQATPDDPGLMSIKDRRDSFCGKMSACNNLTQYGIRYTLTSRHTEAPGSEAFA
                     KDLDAFLRVCRIVGGLRGLRVGAIGARPAAFNTVRYSEKLL"
BASE COUNT          586 a         1568 c         1472 g          565 t
ORIGIN      
        1 ggacccggcc tcgagaagcg gatggtcgta gcgccggagc ccgagcagca ccgagaggcc
       61 gatgaacccg gccgcgctga cgagcgtgcc gcccgcgaag accaggcccc gcgcggcggc
      121 gccggccacg atgaactgcg tggccgccac gcccgcgagg tagcccgcca cgctcttgcc
      181 gaggccgctc aggccgagga tcccgcctcc cagggcgtcc tgcagcagcc ccgtgattgc
      241 gcccgacgcc aggcccgcca cgcggccgtg gcgcagcccg acggcgacca cggccacgag
      301 gacgaggtcg acgaacgcgc cgccggcggc gcccgacagg acgccctgga ggagcaccgc
      361 cgccagcagg aacgccgccg ggccgagggc cgtcttcacc cgccctcctc cgcgcccgcg
      421 cggggcggcg ccacgtcggg ctggcggggc gacacgatca ccaggacgta ctcgaggcgc
      481 gagaagtcca cggcggcctt gatcgtcacg cgcgtgtagg ccgggccggc ccggcggacg
      541 tcgacgacgc gcccgagcgc cagcccggcg ggatagatgc cgtcgaggcc ggccgtcacc
      601 acggcgtcgt ccggctgcac gtcggcggtg gcggagaggt actcgaagcg gagggtcgcg
      661 tcgccgttgc cgagggcgat gccctctgac cgcgtgcgtt cgacgcgtcc cgagacggcg
      721 gcgctgcggt ccacgacgaa ctgcacggtg gaggcggagg cggcggcgat ggcgacgcgg
      781 ccgacgacgc cgccctgcgc gatgaccgcc atgttccggc gcacgccgtc gcgagatccc
      841 ttgtcgatgg tcacggacct gtaatcgggc gacgcgctgc cggcgacgac ccgggcgccc
      901 gtcgtggtcc aggggacgct cgcgcgcacg cccacgagcg cccggaggcc ctcggtgccg
      961 gaagcgctcg cccgggcctc cgccagggcg ccgcgcaggg cctctgcgtc gcgggccagc
     1021 ctcgcgttct cctcccgcac gtgccacagc gccacgtagc cgtccacgat cgatcgcagg
     1081 acgcccacgg cgagccagct cccctcctgc aggcgcgtca cgaggcccac gatgccggtg
     1141 cggagcagcg aggagccgcc cgccgtgccg acctgcgccg agacgagcag cacgtgggcc
     1201 acgatcaccg cggcgagcag gagcggggcg cggctgctga ggtcccggcg cgccatgcct
     1261 cagtcgatcg agatcttgcg cagcaggttg aagtcgccca gcatcttccc ggccccgagg
     1321 acgaccgagg acaggggatc ctcggccatc gccaggggca gcccggtttc ctcgcgcagc
     1381 cgcttgtcga ggttgcgcag gagcgcgccg ccccccgaga gcacgatgcc gcggtccacg
     1441 atgtcggcgg agagctcggg cggcgtgcgt tcgagggcca cgcgcacggc gtcgacgatc
     1501 acgttgattg tctcggccag ggcatcgcgg atctcctcgt cggtgatggt gatggtcttc
     1561 ggcacgccct ccaccaggtg gcggcccttg atctccatgc tgagccgctc gtccaagggg
     1621 aacgccgacc cgatctccat cttgatctgc tcggcggtcc gctcgccgat gagcaggttg
     1681 tggttgcgct tgatgtactg gatgatggac tcgtccatct cgttgccggc gacgcgcacg
     1741 gccttcgagt acacgatgcc cgccagcgag atgaccgcga tgtcgctcgt gccgccgccg
     1801 atgtccacga tcatgttgcc cgtgggctcg gtgatgggca tgcccgcgcc gatggcggac
     1861 gccatcgcct cctcgacgag gtagacctcg ctggccttcg cgcggtacgc gctctccttg
     1921 acggcgcgct tctccacctg cgtgatttcc gacggcacgc cgatcacgat gcgcgggcgc
     1981 acccacatgg cgccgttgtg cgcctgcttg atgaagtgcg tcagcatctt ctcggtgatc
     2041 tcgaagtcgg cgatgacgcc gtccttcatg ggcttgatcg ccacgatgtt gcccggcgtg
     2101 cgcccgagca tgtccttcgc cgcgttgccg accgcctcga ccttgccgtt cagcttgttg
     2161 atggccacaa tcgacggttc gttcacgaca atgccgcgcc cgcgggcgta gacgcaggtg
     2221 ttggcggtgc ccagatcgat ggcgaggtcg ctggagaaca acgagaagag ggaacggaag
     2281 gccaacggtg tgactccttg cggcccggcc tcagcgcggc ggggccaggc agtagctgtt
     2341 cttgccgcgt tccttgacgc ggtacatcgc ctcgtcggcg gcgcgcagca gctcctcggg
     2401 cgccagcgtg acgtccggca gcgtggcgat gcccaccgac gccgtcaggc gcacgtcgag
     2461 gtgcagcggc tgcaggaacg gaaacgccgc cagccggtcc ctgacgcgcc gcgcgaccac
     2521 gacggcgccc tcggcgggcg tgtccggaag cacgacgacg aactcgtcgc cgccgtagcg
     2581 cgccacgatg tcgctctcgc gcgcgcaccc gcggatcacg gtggccgcct cgacgatcgc
     2641 ccggctcccg cacaggtgcc cgtgggtgtc gttcaccgac ttgaacccgt ccagatcgat
     2701 gaacagcacc gacagcggcc ggctgtaccg gaccgcgcgc ttcgtttcgc ggtgcagcgc
     2761 gtcgcggagg aagcgcgagt tgtgcaggcc ggtcagatcg tcggtgaccg acagcgcttc
     2821 ggcctgctgc agcagcaggg cgtggtccag ggccagggcc atcatgccca gcaccgactc
     2881 cagcagcggc cgcacgcccg ccgcgaacct gggccggcgc gcgctcggcc ggcggtcgaa
     2941 cccgacgagc accgcggcgc ggcggccccg tcccgccagg ggcagggcca ccaccgtcgc
     3001 gtcgcccgcc gcctgcacgc gtccgtcccg gcgcaggtcg gccgacacga actccgaccc
     3061 gtgcatcatc acccaggagg cgacgcccag cagcgtcggc tcgtccggcc cctggtggtc
     3121 gcgggcgtgg agaagccgga cctggccggt cccatcgctc gtcagcacag cccatgtatc
     3181 ggcgggaatc cacgccgaga cgcgatccac gagcatgcgg gcgatgcgcg cggggtccgt
     3241 gtccgcgtac gcctcgcgga ccacctcctc gagcaccgag cgccgccgca gcgcgcggcg
     3301 cagctccggc tggccgcgcc gcaggcgctc ggcgaaatcg agcggctggc cgcccgcctc
     3361 accaggactc cgtgtggggg acactcccgc tggctcccgc taagtcgctc agttcccagc
     3421 gaatatagca cagcggcccg ggccagggcg gcccgattcg tcccccattc ggccgcggcg
     3481 gcgccccggg gatgcgcccg accggcggtg cgctacaatc gccgtgcccc tgcccttccc
     3541 gggcctcgtc ccggggtcga gcacctgctc ctcgggggta ccacctcatg gacgctgcga
     3601 acgcacacgc cgccaccctc gggctcatca tcggcaaccg cgggttcttc cccgaccacc
     3661 tcgcccgcag cggacgcgag gagatgctgg cggcgctcgg cgccgccggc gtggaagtcg
     3721 tggcgctggg gcccgaggat tcgaagtgcg gcgcggtgga gacgcccgcc gaggcgcggc
     3781 gctgcgccga cctgttccgg gccaggcgcg acgacatcga cggcgtgatc atctccctgc
     3841 cgaacttcgg cgaggagcgc gccctcgcgg acgcgctgcg cttctcgacg ctcgacgtgc
     3901 cggtcctcgt gcaggccacg cccgacgatc ccggcctgat gtcgatcaag gaccgccgcg
     3961 acagcttctg cggcaagatg tccgcctgca acaacctcac gcagtacggc atccgctaca
     4021 cgctcacctc gcggcacacg gaggcgccgg gctcggaggc gtttgcgaag gacctcgacg
     4081 ccttcctccg cgtctgccgc atcgtcggcg ggctccgcgg cctgcgggtc ggcgccattg
     4141 gcgcccgccc ggccgcgttc aataccgtcc gctacagcga gaagctgctc g
//
LOCUS       DAOSIF010000264         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18757_length_4183_cov_3.991764, whole genome shotgun sequence.
ACCESSION   DAOSIF010000264 DAOSIF010000000
VERSION     DAOSIF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18757_length_4183_cov_3.991764"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLT35_09205"
     CDS             <1..533
                     /locus_tag="PLT35_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOG29444.1"
                     /translation="PTVDDEAVYRVLEKLLVWRHCHKHDCDVHAASGPLLLLGDAFHN
                     FVDGIVLAGAFVASTTLGLAAAVSTVAHEVPQELGEFMVYLRSGYSRKRALVLNSLTS
                     LTTVVGAILGYLFLREMRAVGPYLLAFAAAGFLYVALADLVPSQRGRTSLRLALLDMI
                     LLAAGIGVIALVAHSH"
     gene            586..882
                     /locus_tag="PLT35_09210"
     CDS             586..882
                     /locus_tag="PLT35_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29445.1"
                     /translation="MARRTLPFRVLAVVVFLWVVGLTGARDLLHNHSGFVERPDCPAC
                     RLDGTAGVATPLLGLMVAIPDLRPVGAVPVCPRSSFVSSAAALEPPPRSPPLPL"
     gene            1097..2347
                     /locus_tag="PLT35_09215"
     CDS             1097..2347
                     /locus_tag="PLT35_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG29446.1"
                     /translation="MFWRTSALVALLGVGLPVAPATVAAQTSASAPLTLDDCLRLAAA
                     EHPLLKAAGHRIEAARARVEQAKALPQPTIVYDSDLQPRPFHFVDSGESYLGITQPLG
                     FPGHRKTRADIARREQDTAAADADGVRIVLAYNVRRAFFGLLRAEERLRHLEQDRDLV
                     AGFLKTAETRLAAGDTARVEVVRARVEAAQAATEVQGAAAAVRLARAALNVEMGRPQG
                     EPIAVTGDLRVPPLAESLEDLQQRALVTRPEIRRLGHALDAELLRAKQARQDRWPGFE
                     LGVSSHRVDGARRTWDVTVALPVPLFYGQAAKGPVAEAQANVLAIRRELEHQRHAIGL
                     EVEAAFVEAQLARDQIRLYEEQILAEAEEAHGMLLFSFEQGEIGGFELIASRRTLVAA
                     RLGYADALYNHAVALAAVEMAAGR"
     gene            2371..3642
                     /locus_tag="PLT35_09220"
     CDS             2371..3642
                     /locus_tag="PLT35_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG29447.1"
                     /translation="MRQTRLVIGAALLAAAAWGCTAVGERPAEPPGGRAGQQVLREDA
                     RPGRGHGGAGAGEAERGLRRRGRRWDAAVIRLSAEQEQALGVRTMAVSARPFRTTLRA
                     MGEVTVPLPRKAIVSCAFPARIARVHASVGAWVKRGQAVLTLQSDEVGLAKAEYQKAQ
                     SLLDLARRNCEREKRLFDNGVGAEKTLLASENELKMAEANRLAAERRLRVFGMSDAEI
                     RGLADDRSSPAELTLTAPIEGKVVESAAVLGAMVDASHQILTLVDTRVLWVDAEIYER
                     DIAQVRLGQQATVQVPAYPDEVFEGAVVHVGDIVKPDTRTIGVRVEVSNAGQRLKPGM
                     FASAVFRTGERPSVVALPAQVILDDEDGHLVFVRTPAGYEPRLVDLGTQDNGNREVVR
                     GIAPGDEVVVAGHYQLKSRLHADALRQGASH"
     gene            3650..>4183
                     /locus_tag="PLT35_09225"
     CDS             3650..>4183
                     /locus_tag="PLT35_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG29448.1"
                     /translation="MIARLIASALRHKLLTVLLVALAAVWGALSYARMPKDIYPDLNA
                     PLVKIITENPGMASEDVERLISLPLESLLSGAPGVTRVRSESTTGDSVVTVEFDWGTD
                     IYHARQIVSSKIELIAGRLPQNTTTPVLGAVSSRMGEVFEFAVVGDGIDPMELRSIAD
                     WTIRYQLQGVPGVAFVVN"
BASE COUNT          560 a         1462 c         1533 g          628 t
ORIGIN      
        1 ttccgacggt ggacgacgag gcggtgtacc gcgtgctcga gaagctgctg gtgtggaggc
       61 attgccacaa gcacgactgc gacgtccacg ccgcctcggg acccctcctg ctgcttggcg
      121 acgccttcca caacttcgtg gacggcatcg tcctcgctgg cgccttcgtc gcctcgacga
      181 cgctcggcct ggcggcggcc gtctcgacgg tggcccacga ggtgccgcag gaactcggcg
      241 agttcatggt ctacctccgg agcggctact cacggaagcg ggccctcgtc ctgaacagtc
      301 tcaccagcct gaccacggtc gtcggcgcca tcctcgggta cctcttcctg cgcgagatgc
      361 gggcggtggg gccgtacctg ctcgcgttcg cagccgccgg cttcctctac gtggccctgg
      421 ccgaccttgt tccctcgcag cgcggccgga ccagcctgcg actggcgctg ctggacatga
      481 tcctgctcgc cgccggcatc ggcgtgatcg cgctggtggc ccacagccac tgatgcggac
      541 gcgcgcggct tgaccggacc atccgcgccc gcgtataagg ttgggatggc gcggcgcacg
      601 ctgccgtttc gtgtgctggc ggtggtcgtg ttcctctggg tcgtcgggct cacgggggcg
      661 cgggacctgc tgcacaacca ctccggcttc gtcgagcggc cggattgccc tgcgtgccgc
      721 ctcgacggca ccgcgggcgt cgcgacgccg ctcctcggtc tgatggtcgc catccccgat
      781 ctcaggcccg tcggggccgt gccggtctgc ccgcgttcct cgttcgtatc ctccgcggct
      841 gccctcgagc ctccgccgcg gagtccgcct ctcccgctct agccgtccga tcgccttcat
      901 ctgaacaccc ggcgttcgcg gagcgcctcg gcccgggccg ccgccggccg gcgcgaggaa
      961 gccgctcgcg cggaacgccg ctctgcgccg ttcatccgcc cccgtcaggt gcgccgctcc
     1021 agcctctccc cggccgggcg tcgcggccag ccggacgtgc agccggctcg ctcgtcggtc
     1081 gattcgaggg acgtgcatgt tctggagaac ctcagcgctg gtcgcgttgc tcggcgtcgg
     1141 gctccccgtg gcccctgcga ccgttgcggc gcagacgtca gcatccgcgc cgctgacact
     1201 ggacgactgc ctgcgcctgg ccgccgctga gcacccgctc ctcaaggccg cggggcatcg
     1261 gatcgaggcg gcgcgagcgc gggtggaaca ggcgaaggcg cttccccagc caaccatcgt
     1321 ctacgactcc gacctccagc cgcggccttt ccacttcgtg gactcgggcg aatcgtatct
     1381 gggcatcacc cagcccctcg ggttcccggg gcaccggaag accagggccg acatcgcccg
     1441 tcgtgagcag gacacggcag ccgctgatgc cgacggcgtg cgcatcgtgc tcgcgtacaa
     1501 cgtgcggcgg gcgttcttcg gcttgctgcg cgccgaggag cggctccggc acctcgagca
     1561 ggaccgggat ctcgtcgccg gcttcctgaa gaccgccgaa acccggctgg cggctggtga
     1621 cacggcccgg gtcgaggtcg ttcgagcgcg cgtcgaggcc gctcaagccg cgaccgaagt
     1681 gcagggcgcc gcggcggccg tccggctcgc cagggcagcg ctgaacgtgg agatggggcg
     1741 gccgcagggc gagccgatcg ccgtcacggg cgaccttcgg gttccgcccc tcgccgagtc
     1801 cctcgaggac ctgcagcagc gggccctcgt gacccgcccg gaaatccgcc ggctcggcca
     1861 cgcgctcgac gcggagctgt tgcgcgcgaa gcaggcgcgg caggaccgct ggcccggatt
     1921 cgaactgggc gtctcgagcc atcgcgtcga tggcgcgcgc cggacctggg acgtgacggt
     1981 ggccctgccg gttccgctct tctacggcca ggcggccaag gggcccgtgg cggaggcgca
     2041 ggccaacgtc ctcgcgattc ggcgcgagct cgagcaccag aggcatgcca tcgggctcga
     2101 ggtggaggcg gcctttgtcg aggcgcagct cgcccgcgat cagatccgcc tgtacgagga
     2161 gcagattctc gcggaggcgg aggaggccca cggcatgctg ctgttcagct ttgagcaggg
     2221 cgagattggc ggcttcgagt tgatcgcgtc ccggcgtacg ctcgtcgcgg cgcggctggg
     2281 ctacgccgat gccctctaca accatgcggt ggccctggct gccgtggaga tggcggccgg
     2341 ccggtaggcc ggcgcgcgga gtacggagcc atgcgacaga cacgtctcgt catcggggcg
     2401 gcgctcctgg cggccgcggc ctggggctgc acggcggttg gcgaacgccc ggcggagccg
     2461 ccgggcggcc gtgccgggca gcaggttctt cgcgaggacg cgcgtccggg cagggggcac
     2521 ggcggtgccg gcgccggcga agccgagcgc ggcctccggc gccgggggcg ccggtgggac
     2581 gcggccgtga tccggctctc ggcggagcag gagcaggccc tcggcgtgcg aaccatggcg
     2641 gtctcggcgc gtccgttccg gacgaccctg cgggcgatgg gcgaagtgac ggtgccgctg
     2701 ccccgcaagg cgatcgtgag ctgcgcgttt cccgcccgaa tcgcgcgggt gcatgcctcg
     2761 gtgggggcgt gggtgaaacg cgggcaagct gtcctgacgc tgcagagcga cgaggtgggg
     2821 ctcgcgaagg cggagtacca gaaggcgcag tccctgctgg atctcgcgcg gcggaactgc
     2881 gagcgcgaga agcggctgtt cgacaacggc gtcggcgccg agaagacgct cctcgcgtcg
     2941 gagaacgagc tcaagatggc cgaggcgaac cggctggccg ccgagcggag gctccgcgtc
     3001 ttcggcatgt cggacgcgga gatccgcggc ctggccgacg accgcagcag cccggccgag
     3061 ctgacgctca ccgcgcccat cgagggcaag gtggtggaga gcgccgccgt gctcggggcg
     3121 atggtcgacg cctcgcacca gatcctgacc ttggtcgaca cgcgcgtgct gtgggtcgac
     3181 gccgagatct acgagcgcga catcgcccag gtgaggctgg gacagcaggc aaccgttcag
     3241 gtcccggcgt acccggacga ggtcttcgag ggcgccgtgg tgcacgtcgg cgacatcgtc
     3301 aagcccgaca cgcgcacgat tggcgtgcgg gtggaggtct cgaacgcggg ccagcggctc
     3361 aagccgggca tgttcgcgag cgccgtcttc cggaccggcg agcgcccgtc cgtcgtggcg
     3421 ctgccggcgc aggtcatcct cgacgacgag gacgggcacc tcgtgttcgt gcggacgccg
     3481 gcggggtacg agccgcgcct ggtggacctc ggcacccagg acaacgggaa ccgggaggtc
     3541 gtgcgcggta tcgctccggg agacgaggtg gtcgtcgccg ggcactacca gctcaagtcg
     3601 aggctgcacg cagacgcgtt gaggcagggg gcttcccact aggcacgcca tgatcgcccg
     3661 actgatcgca tccgcgctgc ggcacaaact gctgaccgtg ctcctcgtgg ccctggccgc
     3721 cgtgtggggc gccctgtcct acgcgcggat gccgaaggac atctacccgg acctgaacgc
     3781 gccgctcgtg aagatcatca cggagaaccc cggcatggcg tcggaagacg tcgaacgcct
     3841 gatctcgctg ccgctcgaga gcctgctctc gggcgccccg ggggtgacgc gcgtgcggtc
     3901 tgaatcgacg accggcgact cggtcgtgac ggtggagttc gactggggga cggacatcta
     3961 ccacgcgcgc cagatcgtgt cgagcaagat cgagctcatc gcgggccgcc tgccccagaa
     4021 caccacgacc ccggtcctcg gcgcagtgtc gtcccgcatg ggggaggtgt tcgagttcgc
     4081 cgtcgtgggc gacgggatcg atccgatgga gcttcggtcg attgccgact ggacgatccg
     4141 ctaccagctg caaggcgtgc cgggcgtcgc cttcgtcgtc aat
//
LOCUS       DAOSIF010000265         4179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18775_length_4179_cov_3.463385, whole genome shotgun sequence.
ACCESSION   DAOSIF010000265 DAOSIF010000000
VERSION     DAOSIF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18775_length_4179_cov_3.463385"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLT35_09230"
     CDS             <1..806
                     /locus_tag="PLT35_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29449.1"
                     /translation="RLDRQGCTLVDTTCGSVLNVWKAVRRYAEDGFTSVIHGKVRHEE
                     TQATASQALKPPRGPFLVVLDRAEAAEVCRYIREGGDPAAFLARFAGAYSDGFDPDRD
                     LARVGLANQTTMLKSESLAVGEMFRAAMADRYGLGDLAGHYRAFDTICSATQDRQDAV
                     LSLLRDRPIDLMVVIGGYNSSNTLNLARLCAGRVPTFHVADDECLESPDAIRHRPVGE
                     REEVRARGWLPPAGPIHVGLTAGASTPDNIVGAAILKLAAFAAASSATR"
     gene            862..1302
                     /locus_tag="PLT35_09235"
     CDS             862..1302
                     /locus_tag="PLT35_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOG29450.1"
                     /translation="MCIGNRLAWIGLVLLLAPAAADAQGFKWWQSDRFKAELALTPDQ
                     TARIEDVFQRLLPTLVAGKDALDRLESRLSAVIAEGTAPEADVMRLVDQVEQARSDLT
                     KARTLMLYRMHQVLTPEQRAKMNALHAKREQERRQTRRPPQPEK"
     gene            1340..3004
                     /locus_tag="PLT35_09240"
     CDS             1340..3004
                     /locus_tag="PLT35_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG29451.1"
                     /translation="MNRISTSGLVIGLAAVFALAPALPAFAQAAPPSSADAARVSELV
                     TYARERYEQAKAQPAPAGTPQRSLEEQAASRPVVKLTVAQAVAMALENNIELSVERLN
                     PQLQDLAIAQVRGAYRPTVGSTVNMTSNMPLPTSLLNGGTRVTNEVQNFNANVSQAIS
                     KTGGTLTASFNNSRTNTTSTFSLLNPQYVSQVSATLTQPLLRNFRIDANRQNLRTGAI
                     AREISDVTLRQRIINTTATTKNAYWDLLYSIRAIEAARQSLALAEKLVEDNRVRVEVG
                     TLAPLDIVSAQAEAATRRQTLTAAEAARRTAELTLKRLIVGDTKDPLWTATVDPIDVV
                     DVPAEAVNLDAAVTRALSERTDLVTARKNLASNDIQIQYLRNQMMPALDLTATYGTRG
                     LGGNTFIREGGVVVGEIPGGWSDAFRMMRKVDYPNWTLGVTLSYPIGRSAQDAAYARA
                     KVQYQQAQAQIRALELTVATEVTNAALQVESTLRRLDASRAARQLSEKQLEAVQSKFE
                     VGLSTNFFVVQAQRDLLDAQNTELRALLDYQKALVAFERVQQSAGN"
     gene            3077..>4179
                     /locus_tag="PLT35_09245"
     CDS             3077..>4179
                     /locus_tag="PLT35_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG29452.1"
                     /translation="MKKLIITIIVLGVIAGSVGAYYRYRGTGEQVKVSSLPVTRGEVI
                     ESVGATGTLQAVTTVQVGSQVSGNIKALYADYNSIVKKGQVVAILDPSLIETQIEQSK
                     ANLVKSEAELDRLRVSLEDARTKLKRSESLAARQLLAPQELEAAQLSVRTIEVQIKSQ
                     EASVTQAKASLSQSTVNLQHTVIEAPIDGIVISRSVDVGQTVAASMNAPTLFTIAADL
                     TKMQVNANVDESDVGRIRPGQVVKFRVDAYPLDEFTGSVAQVRLNPTTTQNVVTYATV
                     IDVPNNDLKLKPGMTANVTIEIAKRTDVIRVPNAALRFRPTADTFAALNQPVPPELQR
                     GPGGRGNGAGFAGGAPQGQGAQSQGGRAGQPANT"
BASE COUNT          727 a         1434 c         1442 g          576 t
ORIGIN      
        1 cgcggctgga tcggcagggc tgcacgctcg tcgacacgac ctgcgggtcc gtcctgaacg
       61 tgtggaaggc cgtccgccgc tacgccgagg acgggttcac gtccgtcatc cacggcaagg
      121 tgcgccacga ggaaacccag gcgacggcct ctcaggcgct gaagccccct cggggcccgt
      181 tcctggtcgt tctcgaccgc gccgaggccg ccgaggtctg ccgctacatc cgtgagggag
      241 gcgatccggc ggcgttcctg gctcgcttcg cgggtgccta ctcggacggc ttcgacccgg
      301 accgggacct ggcgcgcgtc ggcctcgcca accagacgac gatgctgaag tcggagtcgc
      361 tggccgtcgg tgagatgttc cgggccgcca tggcggatcg gtacgggctc ggcgatctcg
      421 cgggtcatta ccgggcgttc gacaccatct gcagcgccac ccaggatcgg caggatgccg
      481 tgctgtcgct gctgcgagac cgcccaatcg atctcatggt ggtcatcggg ggctacaaca
      541 gcagcaacac gctgaatctg gcccgtttgt gcgccggccg cgtgcccacc ttccacgtcg
      601 ccgacgacga gtgcctcgag tcgccggacg ccatccggca caggccggtg ggggagcggg
      661 aggaggtccg ggcgcgcggc tggctgccgc ctgccggccc catccacgta ggcctgacgg
      721 ctggcgcctc gacgccggac aacatcgtcg gcgccgccat tctcaagctt gccgccttcg
      781 ctgccgcctc ctccgccacc cggtaaacga cccgtcccgc caagtcgtag tctaggggcg
      841 aagcggaggt agaagaccgc catgtgcatc ggcaatcgtc tggcgtggat tggcctcgtc
      901 ctgctgctcg cccctgccgc cgcggacgcg caggggttca agtggtggca gtccgacagg
      961 ttcaaggcgg agctcgccct gacgccggac cagaccgccc ggatcgagga cgtgttccag
     1021 cggctgctgc ccacgctggt cgccggcaag gacgcgctgg accggctgga gtccaggctc
     1081 tccgccgtca ttgccgaggg gaccgctccc gaggcggacg tcatgcggct ggtggaccag
     1141 gtcgagcagg ccaggtcgga cctgaccaaa gcgcgcacct tgatgctcta ccggatgcat
     1201 caggttctca cgcccgagca gcgcgcgaag atgaacgccc tgcacgcgaa gcgcgagcag
     1261 gagcgccggc agacccgccg cccgcctcag ccagagaagt gaccgcgaca ggttggttcc
     1321 gtcacccggg agggattccg tgaacaggat ttcgacgtct ggactcgtga tcggactggc
     1381 ggcggtcttc gccctcgccc cggcgctgcc ggcgttcgcg caggcggccc cgccgtcgtc
     1441 ggccgacgcg gcgcgggtca gcgaactggt aacgtacgcg cgcgagaggt acgagcaggc
     1501 caaggcgcag ccggctcccg ccggcacgcc gcagcgctcg ctcgaggagc aggccgcgtc
     1561 gcgtccggtc gtgaagctga cggtggccca ggccgtcgcg atggccctcg agaacaacat
     1621 cgagctgtcg gtcgagcggc tgaatccgca gctgcaggac ctggcgattg cgcaggtccg
     1681 gggcgcctac aggccgaccg tgggttcgac cgtcaacatg acgtcgaaca tgccgctgcc
     1741 gacgagcctg ctgaacggcg gcacccgcgt gaccaacgaa gtgcagaact tcaacgcgaa
     1801 cgtctcgcag gcgatctcga agacgggggg cacgctcacg gcgtcgttca acaactcgcg
     1861 cacgaacacg accagcacgt tctcgctgct caacccgcag tacgtgtccc aggtgtcggc
     1921 gacgctcacg cagccgctgc tgcggaactt ccgcatcgac gcgaaccggc agaacctgcg
     1981 aacgggggcc atcgcgcgcg agatcagcga cgtcacgctc cgccagcgga tcatcaacac
     2041 gacggcgacg accaagaacg cctactggga cctgctctac tcgatccgcg cgatcgaggc
     2101 ggcgaggcag tcgctggccc tcgccgagaa gctggtcgag gacaaccggg tccgcgtcga
     2161 ggtcggcacg ctcgcgccgc tcgacatcgt ctcggcccag gccgaggcgg ccacgcggcg
     2221 acagacgctc acggcggccg aggccgcgag gcggacggcc gagttgacgc tgaagcgcct
     2281 gatcgtcggc gacacgaagg acccgctgtg gacggctacg gtggatccca tcgacgtggt
     2341 ggacgtgccg gccgaggccg tcaacctgga cgcggccgtc acccgggcgc tcagcgaacg
     2401 gacggacctg gtgacggcga ggaagaacct ggccagcaac gatatccaga tccagtacct
     2461 gcggaaccag atgatgccgg ccctggatct gaccgcgacc tacggcaccc gcggcctggg
     2521 cggcaacacg ttcatccgcg agggaggcgt ggtggttggc gaaattcccg gcggctggag
     2581 cgacgcgttc cgcatgatgc ggaaggtcga ctatcccaac tggacgctcg gcgtcacgct
     2641 ctcctatccc atcgggcgaa gcgcgcagga cgcggcctac gcgagggcga aggtgcagta
     2701 ccagcaggcc caggcgcaga tccgcgccct ggagctgacg gtggcgaccg aagtgacgaa
     2761 cgccgcgctg caggtcgaga gcacactgcg ccgcctggat gcttcgcgcg cggcgagaca
     2821 gctgtcggag aagcagctcg aggccgtcca gagcaagttc gaggtggggc tgtccacgaa
     2881 cttcttcgtg gtgcaggcgc agcgcgatct gctcgacgcg cagaacacgg agctccgcgc
     2941 tctgctcgac tatcaaaagg cgctcgtggc gttcgagcgc gtgcagcaat cggccggcaa
     3001 ctagcgcggc ccggccgccc gccgacgcgg ctggctgccg actgggatcc gccaacctgg
     3061 ttcgggtgaa gtcaacatga agaaactcat catcacgatc atcgtcctcg gcgtcatcgc
     3121 aggttccgtt ggcgcctact accggtaccg cggcaccggt gagcaggtga aggtctcgag
     3181 cctgcccgtg accaggggcg aggtcatcga gtccgtcggc gccaccggca cgctgcaagc
     3241 ggtcaccacg gtgcaggtcg gctcgcaggt gtccggcaac atcaaggccc tctacgccga
     3301 ctacaactcg atcgtgaaga agggccaggt cgtggcgatc ctggacccgt ccctgatcga
     3361 gacgcagatc gagcagtcca aggccaacct ggtcaagtcc gaggccgagc tcgacaggct
     3421 gagggtctcc ctcgaggacg cccggacgaa actgaagcgg agcgagagtc tggcggcgcg
     3481 ccaactcctg gcgccgcagg agctggaggc cgcccagcta tcggtccgga cgatcgaggt
     3541 gcagatcaag tcgcaggaag cgtcggtgac gcaggcaaag gcctcgctca gccagagcac
     3601 ggtcaacctg cagcacaccg tcatcgaggc gcccatcgac gggatcgtca tctcgcgcag
     3661 cgtggacgtc gggcagacgg tggccgccag catgaacgcg ccgacgctgt tcaccatcgc
     3721 ggccgacctc acgaagatgc aggtcaacgc gaacgtcgac gagtcggacg tgggccgcat
     3781 caggcccggg caggtggtca agttccgggt ggacgcctac cccctcgatg agttcaccgg
     3841 ctcggtcgcg caggtgcggc tcaaccccac caccacgcag aacgtggtga cctacgccac
     3901 ggtcatcgac gtgccgaaca acgacctgaa actgaagccc ggcatgacgg cgaacgtgac
     3961 gatcgagatc gcgaagcgca ccgacgtgat tcgcgtcccg aacgctgccc tgcgcttccg
     4021 gccgacggct gatacgttcg ccgcgttgaa ccagccggtg ccgcctgagc tccagcgcgg
     4081 gcccgggggg cgcggcaacg gcgccggctt cgccggcggc gcgccgcagg gccagggcgc
     4141 gcagagccag ggcggtcgcg ccgggcagcc ggccaacac
//
LOCUS       DAOSIF010000266         4153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_18927_length_4153_cov_4.401171, whole genome shotgun sequence.
ACCESSION   DAOSIF010000266 DAOSIF010000000
VERSION     DAOSIF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18927_length_4153_cov_4.401171"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLT35_09250"
     CDS             <1..411
                     /locus_tag="PLT35_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG29453.1"
                     /translation="NGLVFSVGMPLPIFNRNEAGVRRGTAERRQAEAAQRATEARVAL
                     EVRQALDAADVARRRLAYIEGEYLRSAREARDIVLASYRSGAASLIDYLDAERALREA
                     LRTRNRARFDHRVSVFQYEAAVGAPAPGPERNRP"
     gene            408..1637
                     /locus_tag="PLT35_09255"
     CDS             408..1637
                     /locus_tag="PLT35_09255"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG29454.1"
                     /translation="MTIERTALALLAALALAASGACGGNSRSRPEAGDHDAGPHEDAS
                     AGRNIVHVAPDLQRKWGIGVAEAGRAALPGTITVPGVLALDPQRSGQVSSPLDGKVAS
                     VGAQVGDEVRRGQALVTVHAPALAQAKTAYLQAAARLALTRRESERADVLLEREAIDQ
                     QEHLRRRTAYENASSEFAAAESSLHSYGLDQAAVERLLQQARRPDAAGAALDGIAEPY
                     LRLASPTSGRVIERDVVVGQHVEPRQTLLVVADLSTLWALLDAREADLPLVARGQAVR
                     ITAGVYPGRAWSGRVDHVGDVVDEKTRTVKVRVVVRNDERLLKPNMFVQGEFPTAAVR
                     DALTVPLDAVQTIGGEPVVFVREGAGRFAARPVEIGGRAGDRRIVLKGLDEGEAVVVA
                     GAFTLKAELLKSTLAGE"
     gene            1658..>4153
                     /locus_tag="PLT35_09260"
     CDS             1658..>4153
                     /locus_tag="PLT35_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOG29455.1"
                     /translation="MLERLMALPLRNRLVVALGVLLIVALGAYAVRSIPIDAFPDVTN
                     VQVEVVSTATGMAPLEIEQFVTRPIENGMRGLPGLLTMRSVTKYGLSVITLVFGDDVD
                     IYFARQQVHERLSGVERALPDGVETEMGPIATAMGEIYQYTLEGDPPAGPGGEVARLT
                     RLRTIQDWVVAPLLKSVAGVTEINSFGGYLQQYHVVADPDALLAYGLSVDEVADAIRS
                     NNANVGGGIVSQGPEQRIIRGVGLFRDEEDIRSVVLKAERGTPVTIREVGDVRAGHAV
                     RQGASQMNGDREVVGGIVMMLRGENSREVSKNVEARVREINASRMLPDGLRIVPFHRR
                     SEIVGASTRTVLKALVEGSVLVLAVLAVFLWSVRGALVVIAALPVSALLTFIAMRFAG
                     LGANLMSLGGLAISVGMVIDATIIQVENIQRHLARHPPGPEKLRTVLRAALEVRKPSI
                     FGELIIALTFVPIAAMQGMEGKMFSPLAYTVALALLSSLLLSIFVIPTLCLAVLEPVR
                     RESPVFALARRLYLPWLSITLRRRRAVLGAAGAALIAALLLVPRLGTEFVPVMDEGAF
                     DMDVQMIPGVSLEQAMQTAGEVEARLKRFPELETIVSRTGQTGVAIEARGVDKTGFVG
                     TLLPRSRWTTARSRDELIARMRDSLADIPGMVFSFSQPIQCRIDELVAGTRAQVILKL
                     FGDDADLLRRKAGELASVLGAVPGAADLVVERVSGQPYLAVTVDREKVARYGANAGDV
                     LRVIEIAVAGKPLSRLYQQNRVFDIALRFPDERRGSVEALGALLVAVPGGYRVPLREL
                     ADVRAIEGPAQISRENGQRRIGLELNVVGRDIGG"
BASE COUNT          568 a         1506 c         1488 g          591 t
ORIGIN      
        1 aacggcctgg tgttctccgt cggcatgccc ctgccgatct tcaaccgcaa cgaggccggc
       61 gtcaggcgcg ggacggccga gcggcgtcag gccgaggccg cgcaaagggc caccgaggcg
      121 cgcgtcgccc tcgaggtccg gcaggcgctg gacgccgcgg atgtcgcccg ccggcgcctg
      181 gcctacatcg agggcgagta cctccgcagc gcccgcgaag cgcgcgacat cgtgctcgcg
      241 tcgtaccgga gcggcgcggc ctcgctcatc gactatctgg acgcggagcg cgcgcttcgc
      301 gaagcccttc gcacccggaa ccgcgcgcgg ttcgatcacc gggtcagcgt ctttcagtac
      361 gaggccgccg tgggagcgcc cgcgccgggc ccggaaagga accggccatg acgattgaac
      421 ggaccgccct cgccctgctc gcggccctcg cgctcgcggc gtcgggcgcg tgcggcggga
      481 acagccgcag ccggccggag gccggcgatc acgacgccgg gcctcacgag gacgcctccg
      541 ccgggcgcaa catcgtccac gtggcgcccg accttcagcg caagtggggc atcggcgtcg
      601 cggaggccgg ccgcgccgcg cttcccggca ccatcacggt cccgggcgtc ctggcgctcg
      661 acccgcagcg cagcggccag gtgtcgtcgc ccctcgacgg caaggtggcc tccgtcgggg
      721 cccaggtcgg cgacgaagtg cgccgcggcc aggccctcgt cacggtgcac gcgccggccc
      781 tcgcccaggc caagaccgcc tatctgcagg ccgcggcgag actcgcactc acccgccggg
      841 agtccgagcg ggccgacgtc ctcctcgagc gggaggccat cgaccagcag gagcacctcc
      901 ggcggcggac cgcgtacgag aacgcctcga gcgagttcgc cgcggccgag tcgagcctgc
      961 attcgtacgg cctcgatcag gccgccgtcg agcggctcct gcagcaggcc cgacggcccg
     1021 acgccgccgg cgcggcgctc gacgggattg ccgagccgta cctgaggctg gccagcccga
     1081 cctccggccg cgtgatcgag cgggacgtgg tcgtcggcca gcacgtcgag ccgcggcaga
     1141 cgctgctggt cgtggcggac ctttcgacgc tctgggcgct gctcgacgcg cgtgaggccg
     1201 acctgccgct cgtcgcccgc ggccaggccg tgcgcatcac cgccggcgtc tacccaggcc
     1261 gcgcctggtc ggggcgtgtc gatcacgtcg gcgacgtggt cgacgagaag acccgcacgg
     1321 tcaaggtccg ggtcgtggtc cgcaacgacg aacgcctgct gaagcccaac atgttcgtcc
     1381 agggcgagtt tcccaccgcc gccgtgcgcg acgcgctgac cgtgccgctc gacgcggtcc
     1441 agacgatcgg cggcgagccc gtggtcttcg tccgcgaggg ggccggccgc ttcgccgcgc
     1501 gcccggtgga gatcggcggc cgcgccggcg atcgccggat cgtgctgaag gggctggacg
     1561 agggcgaggc ggtggtcgtc gccggcgcgt tcacgctgaa agcggaactc ctgaaatcga
     1621 cgctggctgg cgaatagccg cctggaggca cgttcccatg ctcgaacgac tgatggcgct
     1681 gcccctcagg aaccggctcg tcgtcgcgct cggcgtcctg ctgatcgtcg cgctcggcgc
     1741 ctacgccgtc cgctccatcc cgatcgacgc gttcccggac gtgaccaacg ttcaggtcga
     1801 ggtggtgagc acggccaccg gcatggcacc gctcgagatc gagcagttcg tcacccgccc
     1861 aatcgagaac ggcatgcgcg ggttgcccgg cctgctgacg atgcgatcgg tgaccaagta
     1921 cggcctgtcg gtgatcacgc tggtcttcgg cgacgacgtg gacatctact tcgctcggca
     1981 gcaggtgcac gagcgcctgt cgggcgtcga gcgggcgctt ccagacggcg tggagaccga
     2041 gatggggccc atcgcgacgg cgatgggcga gatctaccag tacacgctgg agggcgatcc
     2101 gcccgccggc cccggcggcg aggtcgccag gctgacgcgg ctgcggacga tccaggactg
     2161 ggtggtggcg ccgctgctca agagcgtggc cggcgtcacc gagatcaatt ccttcggcgg
     2221 ctacctgcag cagtaccacg tcgtggccga cccggacgcg ctgctcgcgt acggcctctc
     2281 cgtggacgag gtcgccgacg cgatccgcag caacaacgca aacgtcggcg gcggcatcgt
     2341 ctcgcagggg ccggagcagc gcatcatccg aggcgtgggg ctcttcaggg acgaggagga
     2401 catccgcagc gtcgtgctga aagccgagcg cggcacgccg gtgaccatcc gcgaggtcgg
     2461 ggacgtccgc gcgggccacg ccgtccggca gggcgcgtcg cagatgaacg gcgatcgcga
     2521 ggtcgtgggc ggcatcgtga tgatgctccg cggcgagaac agccgcgagg tctccaagaa
     2581 cgtcgaggcc cgggtccgcg agatcaatgc cagccgcatg ctcccggacg ggctgcgcat
     2641 cgtcccattc caccggcgat cggagatcgt cggcgcgagc acgcgcaccg tgctcaaggc
     2701 cctcgtcgag ggctccgtcc tggtgctcgc cgtcctcgcg gtcttcctgt ggagcgtccg
     2761 cggggcgctg gtcgtgattg ccgcgctgcc cgtctcggcc ctcctgacct tcatcgcgat
     2821 gcgcttcgcg gggctggggg cgaacctcat gtccctcggc ggcctggcca tttccgtcgg
     2881 catggtcatc gacgcgacca tcatccaggt ggagaacatc cagcgccacc tcgcccggca
     2941 tccgcccggg ccggagaagc tccgaaccgt gctccgggcg gctctcgagg tccggaagcc
     3001 gagcatcttc ggcgagctga tcatcgccct gacgttcgtg cccatcgccg ccatgcaggg
     3061 catggagggc aagatgttct cgcccctggc ctacacggtg gcgctggcgc tgctctcgtc
     3121 gctcctgctc tccatcttcg tgatccccac gctgtgcctg gcggttctcg agccggtccg
     3181 gcgggagagc ccggtgttcg ccctggccag gcgcctctac ctgccgtggc tctcgatcac
     3241 gctgcgccgg cgccgcgcag tgcttggggc cgccggcgcc gcgcttatcg cggccctgct
     3301 cctggtgccg cggctgggca ccgagttcgt gcccgtcatg gacgagggcg ccttcgacat
     3361 ggacgtccag atgatccccg gcgtgtcgct cgagcaggcc atgcagaccg caggcgaggt
     3421 cgaggcgcgc ctcaagcggt tccccgagct cgagaccatc gtctcccgga ccggccagac
     3481 cggcgtggcg atcgaggcgc ggggcgtgga caagaccggg ttcgtcggca cgctcctgcc
     3541 acggtcgcgg tggacgacgg cccgctcgcg cgacgagctg atcgccagaa tgcgggactc
     3601 gctcgccgac atccccggca tggtcttcag cttcagccag ccgatccagt gccgcatcga
     3661 cgaactcgtc gcgggcaccc gcgcgcaggt cattctcaag ctgttcggcg acgacgccga
     3721 cctcctgagg cggaaggccg gcgagctggc atcggtgctc ggcgccgtcc ccggcgcggc
     3781 cgacctggtg gttgagcgcg tgtccggcca gccgtacctc gccgtgaccg tcgatcgcga
     3841 gaaggtcgcc cgctacggcg ccaacgccgg cgacgtcctg cgggtgatcg agatcgccgt
     3901 cgccggcaag ccgctgtcgc gcctctacca gcagaaccgg gtcttcgaca tcgcgctccg
     3961 ctttcccgac gagcgccggg gctcggtcga ggcgctcggc gcgctcctcg tcgcggtgcc
     4021 cggcgggtac cgcgtgcccc tgagggagct cgccgacgtg agggcgatcg aaggccccgc
     4081 gcagatcagc cgggagaacg gtcagcggcg catcggcctg gagctgaacg tcgtcgggcg
     4141 cgacatcggc ggg
//
LOCUS       DAOSIF010000267         4140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19020_length_4140_cov_3.964259, whole genome shotgun sequence.
ACCESSION   DAOSIF010000267 DAOSIF010000000
VERSION     DAOSIF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19020_length_4140_cov_3.964259"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLT35_09265"
     CDS             <1..463
                     /locus_tag="PLT35_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29456.1"
                     /translation="DRLEREIAPHSEWLIELALSSPRLAIRAITTDPLPGGMWRVRLV
                     VENTGWLPTNGAQQAIESGAVQPVSVELTMPAGARLVAGEPRQRLGQLAGRSEQRSTA
                     TPWGYRPGTPDRAFAEWIVAAAPGSRLALKASHERAGVVRSEVTLPSPAVR"
     gene            506..1909
                     /locus_tag="PLT35_09270"
     CDS             506..1909
                     /locus_tag="PLT35_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOG29457.1"
                     /translation="MRISLATAAACLFAITAGAQAPPPSEAPVGRKLIEIYRVAPGQH
                     EAFLRAIAQCDEANRRAGVPPRQLYVHSDGASWDFLLIQDAEYPEGKGALVDKAFEDL
                     RLPTGARFFIDFRRFILEHTDTFASGPTTASAYLATLHATRPAVPLGGGLTLSYELRK
                     SIPLGGTPRWDYLTVDPAARRVYVAHDVNVEVVDLDEQRVVGQVRGLAGAHGIALAPE
                     MGRGFVASGDRNLVTAFSLETLQHLGVAKVGRGPDSVTFEPVTRSVLAWNGDSQNVTI
                     LDAGSLRVVHTVPLGGTPEFAVADGAGSVFVNIEETHEIVTVDAGRGVVAARRAIAGC
                     DEPTGLAFDATARRLFSACSNATLVSVDAATGARLGSAPIGRGADAVVWDESRGIVFV
                     SSSEGFVSLVEVGQDGSLRSLAAVQTRVTGRTMALDPATGAIYVPAADLDIDWDTRAG
                     AFARDGLKLHVFEPVRR"
     gene            complement(2205..2441)
                     /locus_tag="PLT35_09275"
     CDS             complement(2205..2441)
                     /locus_tag="PLT35_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG29458.1"
                     /translation="MFYAETATLVEQARQRFVRKWRLQYPAVVEWLEEAGEELLTFLR
                     FPRTQWRALRTTNAPERFNEESRRRTKTQASLPS"
     gene            complement(2414..3169)
                     /locus_tag="PLT35_09280"
                     /pseudo
     CDS             complement(2414..3169)
                     /locus_tag="PLT35_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3681..4140
                     /locus_tag="PLT35_09285"
                     /pseudo
     CDS             3681..4140
                     /locus_tag="PLT35_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903157.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          660 a         1456 c         1365 g          659 t
ORIGIN      
        1 ggaccggctc gagcgcgaga tcgcacccca ctcggagtgg ctgatcgagt tggcgctgtc
       61 gtcgccaagg ctggcgattc gagcgatcac cacggacccg ctcccgggcg ggatgtggcg
      121 cgttcgtctc gtcgtcgaga acaccgggtg gcttccgacc aacggcgcgc agcaggcaat
      181 cgagagcggc gcggtgcaac ccgtatcggt tgagctgacg atgccggcgg gtgcccgcct
      241 ggtagcaggc gagccgcgtc agcggctcgg ccagctggcg ggccgcagcg agcagcgctc
      301 gacggctacc ccatggggtt acagaccggg cacgccagat cgggcgttcg ccgaatggat
      361 cgtcgccgcg gcccccggga gccggctcgc gttgaaggcg agtcacgagc gagcgggcgt
      421 ggtccgctcg gaggtgacgc tgccgtcgcc cgccgtgcgc tagctcgagc cgtgagactg
      481 cacctgccaa caggagggat cacccatgag gatcagcctc gccacagcgg ccgcctgcct
      541 ctttgccatc accgcgggcg cccaggcccc gccaccctcg gaggcgcccg tcggccgaaa
      601 gctgattgag atctatcggg tggcaccggg gcagcacgaa gcgttcctgc gggccatcgc
      661 gcagtgcgac gaggcgaacc ggcgggccgg cgttccgcca cggcagctct acgtgcacag
      721 cgacggagcc agttgggact tcctcctgat ccaggacgcc gagtaccccg aagggaaggg
      781 cgcgctcgtg gacaaggcgt tcgaggatct gcggcttccg accggcgcgc gctttttcat
      841 cgacttccgc cggttcatcc ttgagcacac cgacacgttc gccagcggcc cgacgaccgc
      901 ctccgcatac ctcgcgacgc tccacgcgac acgcccggcg gtcccgctcg gcgggggtct
      961 gacgctgtcg tacgagctgc ggaagtcgat tccgctcggc ggaacgccgc gctgggacta
     1021 cctgacggtc gatccggcgg cccgccgtgt ctacgtggcg cacgatgtga acgtcgaagt
     1081 ggtggacctc gacgaacagc gcgtggtcgg ccaggtgcgc ggacttgccg gtgctcacgg
     1141 gatcgcgctg gcgcccgaga tgggacgcgg cttcgtcgcc agcggcgacc gaaatctggt
     1201 cacggcgttc agcctggaga cgctgcagca cctcggcgtg gcaaaggtcg ggcgcggacc
     1261 ggacagcgtc acgttcgagc cggtgacgcg cagcgttctg gcctggaacg gcgacagcca
     1321 gaacgtgacg atcctcgacg ccggctccct gcgggtcgtc cacacggtac cgctcggggg
     1381 aacgcccgag ttcgccgtcg cagacggtgc cggctcggtc ttcgtgaaca tcgaggagac
     1441 tcacgaaatc gtcacggtcg atgcgggccg tggcgtggtg gccgcgcgcc gggcgatcgc
     1501 cgggtgcgac gagcccacgg gactggcgtt tgacgcgacc gcccgccggc tgttcagcgc
     1561 ctgctccaac gcgacgctgg tgtcggtcga tgccgcgacc ggcgcccggc tgggctcagc
     1621 ccccatcggg cgcggagcgg acgcggtcgt ctgggacgaa tcacgcggga tagtcttcgt
     1681 gtcgagcagc gaaggcttcg tatcgctggt cgaggtcggc caggacgggt cgctgcgctc
     1741 cctggcggcg gtgcagacgc gggtgacggg ccgcaccatg gcgctcgatc cggcgaccgg
     1801 ggcgatctac gtgccggccg ccgacctgga catcgactgg gacacccgcg cgggcgcgtt
     1861 cgcccgggac ggcctcaagc tccacgtgtt cgagccggtc cggaggtgac gcgggtgaca
     1921 agtccgctct ccgaaacgct ccgcgacggt gtcacgaccg tgaccgtcgc gtgcaaccac
     1981 gactgcggtg gctagtgcca cctgaaggcg catgtacgtg acggcggctg tgtctgtcaa
     2041 gtggtggaga agtgtgaagt catcaccggc tgagaaagat caactgacaa ggaagagcct
     2101 cacgcggcct ccttcaccgt cttcatctct tgccacccat cgatcgctcg caaccggatc
     2161 ttcccgctgc gcaggagccc gaacagcaac aacaacacgg cgtcctagct cgggagactg
     2221 gcttgcgtct tggtccgccg ccgggactcc tcgttgaatc gctcaggcgc gttggtcgtc
     2281 cgcagcgctc gccactgggt gcgcgggaag cggaggaagg taagcagttc ctcgcccgct
     2341 tcttccagcc actccaccac cgccgggtac tgcagccgcc acttgcgcac aaaccgctgc
     2401 cgcgcctgct cgactagcgt tgccgtctcg gcgtagaaca tgcacggtag tcctccgcga
     2461 gttcctcgcg ctgccgcttc gggcccttcg cctccaaatt ccgggacttg tgcgtcgtac
     2521 accgctggac cggcaacgtc ggccactgtt cccgcaacgc ggcgcgcagc cccgcgctct
     2581 catcgatcac cgccagcacc ggcgtgccga actggcactc gatgaggcag cggatcgtgt
     2641 cccgccaggc cgcggtactc tcgccgccgg tcagccgcag gtccaacagg accttccggc
     2701 cgtcggcccg cacgcccagc accaccaaca ctggcacgac cacgcgccgc ttcccgagcc
     2761 gcaccttcgg ataccagccg tccaggaaca gatagcggat ctgctccgcc gccagatcgc
     2821 gggtccgtca ctgctcgaat tcctccttca gccggcctac caaccgcgac accgcgtcct
     2881 tagacaaggg cccgttctgc agcaatggca ccagcgtcca gcggacccgc cggccattgg
     2941 tcccgctcag gtagacgtca aagagcgcct catagacccg cgcggtccgc cgctgatacc
     3001 gggccaccgt ggcactgcgc cattccgtcg tcccccccgc ctcctcgaac agccgcgccc
     3061 gcggcaacgc gaacgtcgtc gggctcaggc tggtcgtcag ggtccgcggt cccgcccagt
     3121 gccggtagcc ccggccgcgt ctgcccgacc cggaccgaac tctccgcacc cagcaccgtc
     3181 gcgagttcct ggtccacgat cacttcgatc cacctccggc cctgctcccg aatccccgcc
     3241 tcccgccgat cccgatcgcc gcacagatct tacttttcaa tgctcctctt ggcatggtcg
     3301 gccatcgcgt agtcctccgg cacccgccgc caagcgggcg cagctcttgg cttgaactgc
     3361 gccggatgat gacgcgattc ctacttttcc accaccttca cgacactacc caacgaactg
     3421 atcccagcct cgtttgtctg gacaattgcc atcgccgcgg ggcggacgtc gtcgtacctt
     3481 gcatggtccg agtcgtcagc agagaggtcg tggtacacgt agtccgtgcg atcccgcact
     3541 tagcaggcca agaacgcgtt agagtcctag gacgtgctgg aaattcggta ggccgctgag
     3601 gctggacaag caagcggcca tcgcgcatcg ttccgtgagt tttctctggc aggagaagac
     3661 gacacggaag tgatgcgacg atggccaagc ccagcatgga tttctcgacg ttcgttggca
     3721 agctcctcga ggagcaagat ggggacgtcc tccgagaagg cgtccgggcc tgcccaggcg
     3781 gtcatggatg cggaggtgac ggccctggtc ggtgctgaac gccacgagcg gagcgatgat
     3841 cggacggcct accggaacgg gacccgggtc cggacgtggg acacgcgggt ggggacggtg
     3901 gagctggcga tctccacggt gcgtcccggc agttccttcc catccctgct gcagccgcgg
     3961 cgccgcgccg agcaggccct cctcacgatc atccaggaag cctatgtgcc cggcgtgtcg
     4021 acccggaagg ttgacgacct ggtgacgggc ctggggctcg acggcatctc caagtcgaga
     4081 gtctcgcgca tctgtgcgga gctcgatccg atcgtgaagg cgtttcgccc acgccccctg
//
LOCUS       DAOSIF010000268         4136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19038_length_4136_cov_4.478559, whole genome shotgun sequence.
ACCESSION   DAOSIF010000268 DAOSIF010000000
VERSION     DAOSIF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19038_length_4136_cov_4.478559"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLT35_09290"
     CDS             <1..443
                     /locus_tag="PLT35_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29459.1"
                     /translation="ALSVLAGIVGIAAAYRIFVRAPEIAETLARRWSGAHRVLSNKYY
                     VDELYGATVVAGTMGAARGLWAFDRSVVDGAVNGSGWLTVSSAWVSHALDKYIVDGLV
                     NLVGAVLEAGSFVVRRVQTGLVQNYALVMLFGVFAFVSVYLFVR"
     gene            487..2172
                     /locus_tag="PLT35_09295"
     CDS             487..2172
                     /locus_tag="PLT35_09295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOG29460.1"
                     /translation="MSNAANFPILSTILFTPIAGAVLLLFVNKRQENLIRWIANLVAL
                     LGFLVSVPIWFWYDPAGPQYQFIERVPWIPSIGADYFLGVDGFSVLLVLLTTLMGAIA
                     VLSSWTAITDRVKEYYVFLLLLQGGMIGAFVALDFLLFFLFWEVMLVPMYFLIGIWGS
                     DRRLYSAIKFFLYTLVGSVVMLLGILALYFHLHDVTGAYSFDITKFQAMHAPFDLQWW
                     VFLAFFLGFSIKVPMFPFHTWLPDAHTDAPTAGSVILAAVLLKMGTYGFIRFSLPILP
                     EATKASIWWVAGLAIIGIIYGALVALAQKDWKRLVAYSSVSHMGMVMLGLITLNPVGI
                     TGSIIQQLNHGISTGALFLIVGIVYERRHTREIAEYGGLSKVMPVFAAIFMVMMLSSL
                     GLPTLNGFIGEILILQGAYTANIWWAAVAASGLVLGAAYLLWLYQRTMFGRLENPKNE
                     HLRDLSLREIVTFAPLVVLAVWIGIYPAPFINRTAPTVQAVMQRVNPEYARASAAAGA
                     RQAPKIADCDENEPLTPFTTAPCGDAAEGQAPPPPAAPEGGGAPQANPAGGRH"
     gene            2172..3647
                     /locus_tag="PLT35_09300"
     CDS             2172..3647
                     /locus_tag="PLT35_09300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOG29461.1"
                     /translation="MPAWFSLNDLYYLLPELVLASGVLALLVATALTPKRRQGWLSWL
                     ALGALAASAAAVLAVARPVPVDVARGLISVDAFAAFFKGIVLLAAALTVLLSVRYLDT
                     EGARHGSYMFLVLCATLGMMFMASGTDLVVIFVGLETMAIAFYILAGLLKPDRRSNEA
                     AVKYFLLGAFSLGILLYGISILYGMSGTTNLRALAEAVAARGRDPWLMLALILVAAGI
                     GFKIAAVPFHMWAPDVYEGAPTPITAFLSVGSKAASFAMLLRIFLEGLPSLSGDWTIL
                     FYVLAVVTMTVGNIAALTQSNTKRMLAYSSIAHAGYLLIGVIAGTTRGVTAMLIYFVI
                     YAFMQMGAFAVVVLLRRADVIGDELKDLGGLLARQPFAAVAMLVFMLSLGGIPPTAGF
                     MGKFWLFSAAIDANYVWLAVIGVLNSAVSLYYYLRVVVFMFMKHEAAGSQPALSPALT
                     TALVVTLAGTLVLGVYPRPLFELAELSARALGVVGVSATVF"
     gene            complement(3833..>4136)
                     /locus_tag="PLT35_09305"
     CDS             complement(3833..>4136)
                     /locus_tag="PLT35_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOG29462.1"
                     /translation="RAWPAAERDVFYRYAYPIGPDVFVLWDREPAGWAPQNHSCNPNT
                     AFSGLNLVALRDIRAGEELTVDYGTFYDGRMTPFDCACGSPSCRGRIVGGRGLFGS"
BASE COUNT          550 a         1452 c         1375 g          759 t
ORIGIN      
        1 tggccctttc ggtgctcgcg ggcatcgtgg gcatcgcggc cgcctaccgc atcttcgtgc
       61 gggcaccgga gatcgccgag accctggccc gccgctggtc gggcgcgcac cgggtgctgt
      121 cgaacaagta ctacgtggac gagctctacg gcgccaccgt cgtcgccggc acgatgggcg
      181 cggccagggg gctctgggcc ttcgaccgca gcgtggtgga cggggcggtg aacggatcgg
      241 gctggctgac ggtttcctcg gcctgggtct cgcacgccct cgacaagtac atcgtggacg
      301 gcctcgtgaa cctcgtcggc gcggtgctcg aggcgggcag cttcgtcgtc cgccgcgtcc
      361 agaccggcct cgtgcagaac tacgcgctcg tcatgctgtt cggcgtgttc gcgttcgtga
      421 gcgtctacct gttcgtgcgg tagccggagt cggatttcag gtgcctctaa ccccaggact
      481 gcagcagtga gcaacgccgc caacttcccg atcctgtcca ccatcctctt cacgccgatt
      541 gcgggggccg ttctgctgct cttcgtgaac aagcgtcagg agaacctgat ccgctggatc
      601 gcgaacctcg tcgcgctcct cggattcctg gtgtcggtgc cgatctggtt ctggtacgac
      661 ccggcgggcc cgcagtacca gttcatcgag cgggtcccgt ggatcccctc catcggcgcc
      721 gactacttcc tcggcgtgga cggcttcagc gtgctgctgg tcctgctgac caccctgatg
      781 ggcgccatcg ccgtgctctc gtcctggacg gccatcacgg accgcgtcaa ggagtactac
      841 gtcttcctgc tgctgctcca gggcggcatg atcggcgcct tcgtcgcgct cgacttcctc
      901 ctgttcttcc tgttctggga ggtcatgctg gtgccgatgt acttcctcat cggcatctgg
      961 ggcagcgacc gccggctcta ctcggcgatc aagttcttcc tctacaccct ggtcggcagc
     1021 gtggtgatgc tgctgggcat cctcgccctg tacttccacc tccacgacgt caccggcgcg
     1081 tacagcttcg acatcacgaa gttccaggcg atgcacgcgc cgttcgacct gcagtggtgg
     1141 gtgttcctgg cgtttttcct gggcttctcc atcaaggtcc cgatgttccc gttccacacc
     1201 tggctgcccg acgcgcacac cgacgcgccg acggccgggt cggtcatcct ggcggccgtg
     1261 ctcctgaaga tggggacgta cgggttcatc cggttcagcc tgccgatcct gcccgaggcc
     1321 acgaaggcca gcatctggtg ggtggccggc ctcgcgatta tcggcatcat ctacggcgcg
     1381 ctggtcgcgc tggcccagaa ggactggaag cgcctggtcg cgtactccag cgtcagccac
     1441 atgggcatgg tgatgctggg cctgatcacc ctgaacccgg tcggcatcac cggcagcatc
     1501 atccagcagc tgaaccacgg gatctcgacc ggcgcgctct tcctcatcgt cggcatcgtg
     1561 tacgagcggc ggcacacccg cgagatcgcc gagtacgggg ggctctcgaa ggtcatgccg
     1621 gtcttcgccg cgatcttcat ggtcatgatg ctctcgtcgc tgggcctgcc gacgctcaac
     1681 ggcttcatcg gcgagatcct gatcctgcag ggcgcctaca ccgccaacat ctggtgggcg
     1741 gcggtcgcgg ccagcggcct ggtcctcggc gcggcctacc tcctgtggct ctaccagcgg
     1801 acgatgttcg gcaggctcga gaacccgaag aacgagcacc tgcgcgacct gagcctgcgc
     1861 gagatcgtca ccttcgcgcc gctcgtcgtc ctcgcggtct ggatcggcat ctacccggcg
     1921 ccgttcatca accggacggc tccgaccgtg caggccgtca tgcagcgcgt gaatcccgag
     1981 tacgcgcggg cctcggcggc ggcgggcgcg cgccaggcgc cgaagatcgc ggactgcgac
     2041 gagaacgagc cgctgactcc gttcacgacc gcgccctgcg gcgacgctgc ggaggggcag
     2101 gcccccccgc cgccggccgc ccctgagggc ggcggcgcgc cgcaggcgaa tccggcagga
     2161 gggcggcact gatgcccgcg tggttctcgc tgaacgacct ctactatctg ctgccggagc
     2221 tggtgctcgc ctccggcgtg ctcgccctgc tcgtcgccac ggccctgacg cccaaaaggc
     2281 gccagggctg gctctcgtgg ctggcgctgg gcgcgctggc ggcctcggcc gccgccgtgc
     2341 tggccgtcgc ccggcccgtc ccggtggacg tcgccagggg cctcatctcc gtggacgcct
     2401 tcgccgcgtt cttcaagggg atcgtcctgc tcgccgccgc tctcaccgtc ctcctgtccg
     2461 tgcgctacct cgacaccgag ggcgcgcggc acggctccta catgttcctc gtgctgtgcg
     2521 ccacgctcgg catgatgttc atggcctcag gcaccgacct cgtcgtgatc ttcgtcggcc
     2581 tcgagacgat ggcgatcgcc ttctacatcc tggccggcct gctcaagccg gatcggcggt
     2641 cgaacgaggc cgcggtgaag tacttcctgc tgggcgcgtt ctccctgggc atcctcctgt
     2701 acgggatttc gatcctgtac ggcatgtccg gcacgacgaa cctccgcgcg ctggcggagg
     2761 ccgtcgcggc ccgcgggcgg gatccctggc tgatgctcgc gctgatcctc gtggcggcgg
     2821 gcatcggctt caagatcgcg gccgtgccgt tccacatgtg ggccccggac gtgtacgagg
     2881 gcgcgcccac gcccatcacg gcgttcctgt cggtgggatc gaaagccgcg tccttcgcga
     2941 tgctgctccg gatcttcctc gagggcctgc cgtctctctc cggcgactgg acgatcctct
     3001 tctacgtcct cgccgtcgtg acgatgaccg tcggcaacat cgcggcgctg acgcagagca
     3061 acacgaagcg gatgctggcg tactcgtcga tcgcccacgc cggctacctg ctgatcggcg
     3121 tgatcgccgg caccacccgc ggcgtcaccg cgatgctcat ctacttcgtg atctacgcgt
     3181 tcatgcagat gggcgccttc gcggtagtcg tcctgctgcg gcgggccgac gtcatcggcg
     3241 acgagctgaa ggacctcggc gggctcctcg cgcggcagcc cttcgccgcc gtcgcgatgc
     3301 tcgtcttcat gctgtcgctc ggcggcatcc cgcccaccgc cggtttcatg ggcaagttct
     3361 ggctcttcag cgcggccatc gacgcgaact acgtctggct ggccgtcatc ggcgtgctca
     3421 acagcgccgt ctcgctctac tactacctgc gcgtcgtcgt gttcatgttc atgaagcacg
     3481 aggcggccgg gtcgcagccg gcgctctcgc cggcgctgac gacggcgctc gtcgtgacgc
     3541 tggccgggac gctggtgctg ggggtgtacc cgcggccgct gttcgagctg gccgagctgt
     3601 cggccagggc gctcggggtc gtcggcgtct cggcgacggt gttctgagag gaatccagaa
     3661 gccagaatcc cgaacggaca ggtgacggtt gggaggttag gagctacagg gggtaaagga
     3721 ggcaggggag gcccgaccag cgccaagcgc ctggtctgcg gccccttgcc ccggtgttga
     3781 cgagttccga gctccgagcc acgaggccag agccccgcgt cccgaatccc catcagctcc
     3841 cgaacagccc ccgcccgccg acgatgcgcc cgcggcagct cgggctgccg caggcgcagt
     3901 cgaacggcgt catgcggccg tcgtagaacg tgccgtagtc caccgtcagc tcctccccgg
     3961 cgcggatgtc ccggagggcg acgaggttca agcccgagaa cgcggtgttg ggattgcagg
     4021 agtggttctg gggcgcccac ccggcgggct cgcggtccca gaggacgaag acgtccgggc
     4081 cgatggggta ggcgtatcgg tagaagacat cccgctcggc ggccggccag gcgcgc
//
LOCUS       DAOSIF010000269         4128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19071_length_4128_cov_3.869138, whole genome shotgun sequence.
ACCESSION   DAOSIF010000269 DAOSIF010000000
VERSION     DAOSIF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19071_length_4128_cov_3.869138"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="PLT35_09310"
     CDS             <1..1654
                     /locus_tag="PLT35_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG29463.1"
                     /translation="AGYEFQFIKTEVQDVNPLYGRDTYSGQFTRPPGAAANNLYNLAD
                     FMLGLRSQYALSNILIAHLRQRMQFVYLQDDFRVNDKLTLNLGLRYEYATPQWETNSL
                     WSNFDKDKVAMVMAKDGSIADRALVEPDRNNFAPRLGFAWTALPKTVVRGGFGVSYVH
                     FNRIGAANVLAINGPQVVNAVVSQTNPTLPAFRTTQQGYPENFADPSQFDPLKANVTY
                     MDRNYHSSEVKSAYISVQRELTSNTVLDVAYVHNRADGMMMVANYNQAVPNNAAASIP
                     LQARRPIQTFGDITYVWNGGKSRYHGLQVKLEYRMRGGLMFLNSFTLSKAKDNGPGAL
                     ENSNSGNVGPQDFYNMGPEYGTSNYDQPVNNTTSFVWELPFGHGRRWLNDAHPILNAV
                     LGGWTISGINTMTSGAPATLVYTPTASWAVSGISQEYRGSNTYRPNVTGSPYGNKKSI
                     TEYLSKTSVTIPADASQVYGNAPRNSVRLPWFWQLDFVASKNFTLPFGRHTTLQVRAE
                     AFNLLNRTNFAGLSTNRTSANFGTITSAFDARQIQLGAKITF"
     gene            1725..2654
                     /locus_tag="PLT35_09315"
     CDS             1725..2654
                     /locus_tag="PLT35_09315"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOG29464.1"
                     /translation="MTTRYLALAACAVTPCLACAVLHAQAPAAKKILVAHRGASAYAP
                     EHSAEAYTLAIAQGADFVEQDLAVTKDGVLVSIHDVSLERTTNVEEVFPDRFVEEATA
                     KGPLKRWYVNDFTLAEIKRLDAGSSFDKKFAGTRMLTFQEAIDLVRGKAGLYPELKDP
                     EFYRRRGVDQVRLFADVVKKNGLEADPKTPLIVQSFDEATLRAVGKLLPSVPRVFLVS
                     PQAADRLDSPAKLREIAAWATGIGPSKLIFEKLPDLAAWAHAAKLTVTPWTFRPPAPP
                     QFPTIREEMSYFLFTLGVDALFTDNPDMFPRTK"
     gene            complement(2746..3516)
                     /locus_tag="PLT35_09320"
     CDS             complement(2746..3516)
                     /locus_tag="PLT35_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HOG29465.1"
                     /translation="MARDTWREVLAEFFGTFVLILFGAGVVAQYVLSRQVNGSYLAIN
                     LTWGLGVALGCYVSIGVSGAHLNPAVTLAVAVHRGFPWRKVLPYVTAQVLGAFVAAAV
                     VYLTYREALAAFDGGVRQVLGPQGTGGIFATYPQPYLSTFPGGFIDQVVGTAILMGVL
                     FGITDPRHAPPAGLVPLIVGLLVVAIGAAFGVNCGYAINPARDFGPRIFTAMAGWGSG
                     VFRAGNSWWWVPIVGPLAGGVLGGWLYDIFVGKPTRAR"
     gene            3634..>4128
                     /locus_tag="PLT35_09325"
     CDS             3634..>4128
                     /locus_tag="PLT35_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOG29466.1"
                     /translation="MAYILALDQGTTSSRAILFDRSGGTSAVAQREFEQHFPTPGWVE
                     HDPREIWASQMAVAAEALARAQAGPGDVAAIGITNQRETTVVWDRQTGEPVYPAIVWQ
                     DRRTAEQCDRLKADGFEPLVRERTGLVIDAYFSGTKVAWILDHVDGARAKAEAGRLAF
                     GTIDA"
BASE COUNT          729 a         1476 c         1333 g          590 t
ORIGIN      
        1 ggcgggatac gagttccagt tcatcaagac cgaggtgcag gacgtcaacc cgctgtacgg
       61 ccgtgacacg tactcgggcc agttcacgag gcctccggga gcggccgcga acaacctgta
      121 caacctcgcc gacttcatgc tgggtttgcg cagccagtac gcgctgagca acatcctgat
      181 cgcccacctc cgccagcgga tgcagttcgt gtacctgcag gacgacttcc gcgtgaacga
      241 caagctcacg ctgaacctcg ggctgcgcta cgagtacgcc acgccgcagt gggagacgaa
      301 cagcctctgg tcgaacttcg acaaggacaa ggtggcgatg gtcatggcga aggacggctc
      361 gatcgccgat cgcgcgctcg tcgagcccga ccgcaacaac ttcgcgcctc gcctgggctt
      421 cgcctggacc gcgctgccga agacggtcgt ccgcggtggc ttcggcgtga gctacgtgca
      481 cttcaaccgg atcggcgcgg cgaacgtgct ggccatcaac gggccgcagg tcgtcaacgc
      541 ggtcgtctcg cagacgaacc cgacgcttcc ggccttccga acgacgcagc agggctaccc
      601 ggagaacttc gccgacccct cgcagttcga cccgctgaag gcgaacgtga cctacatgga
      661 ccggaactac cactcgtcgg aggtcaagag cgcctacatc tcggtgcagc gcgagctgac
      721 gtcgaacacg gtgctcgacg tggcctacgt gcacaaccgc gccgacggca tgatgatggt
      781 cgcgaactac aaccaggcgg tcccgaacaa cgccgccgcc tccatcccgc tgcaggcgcg
      841 ccgcccgatc cagaccttcg gcgacatcac ctacgtgtgg aacggcggga agtcccgcta
      901 ccacgggctc caggtgaagc tcgagtaccg catgcgcggc ggcctcatgt tcctgaactc
      961 cttcacgctg tcgaaggcca aggacaacgg ccccggcgcg ctggagaact cgaacagcgg
     1021 caacgtcggg ccccaggact tctacaacat gggtccggag tacgggacgt cgaactacga
     1081 ccagccggtc aacaacacga ccagcttcgt ctgggagctg cccttcggcc acggacgccg
     1141 ctggctgaac gacgcccacc cgatcctcaa tgccgttctc ggcggctgga ccatcagcgg
     1201 gatcaacacg atgacgtccg gcgcgccggc aaccctcgtg tacacgccga cggcgtcgtg
     1261 ggccgtctcc gggatctctc aggaataccg cgggtcgaac acctaccggc cgaacgtgac
     1321 cggcagcccg tacggcaaca agaagtcgat cacggagtac ctcagcaaga cgtccgtgac
     1381 gattcccgcc gacgccagcc aggtatatgg caacgccccg cgcaactcgg tccggctgcc
     1441 ctggttctgg cagctggact tcgtcgcgtc gaagaacttc acgctcccgt tcgggcgcca
     1501 taccacgctc caggtccgcg cggaggcgtt caacctgctt aaccgcacga acttcgccgg
     1561 cctgagcacc aatcgcacgt cggcgaactt cggcacgatt acgtccgcgt tcgacgcgcg
     1621 ccagatccag ctcggcgcca agatcacctt ctagccccaa ccggcaatag gccgcgggag
     1681 acggccctgg cccgcctccc gcggcctcct cggaggcacg accgatgacg acccgttacc
     1741 tcgcgctggc cgcctgcgcg gtcacgccgt gcctggcgtg cgccgtcctc cacgcgcagg
     1801 ctcccgccgc gaagaagatc ctggtggccc atcgcggggc gtcggcctac gccccggaac
     1861 actccgccga ggcctacacg ctcgccatcg cccagggggc tgatttcgtc gagcaggacc
     1921 tcgcggtgac gaaggacggc gtgctggtct ccatccacga cgtttcgctc gagcgcacga
     1981 ccaacgtcga agaggtgttc ccggaccgct tcgtcgagga ggcgacggcg aaggggccgc
     2041 tgaagcgctg gtacgtcaac gacttcacgc tggccgagat caagcggctc gacgcggggt
     2101 cgtcgttcga caagaagttc gccggcacgc ggatgctgac gttccaggag gctatcgatc
     2161 tcgtccgagg gaaggcgggc ctctacccgg agctgaagga tcccgagttc taccgcaggc
     2221 gcggcgtgga ccaggttcgc ttgttcgccg atgtcgtcaa gaagaacggc ctggaggccg
     2281 acccgaagac ccccctcatc gtccagtcgt tcgacgaggc cacgctgcgg gccgtcggga
     2341 agctcctgcc gtcggtgccg cgcgtcttcc tcgtctcgcc ccaggcagcc gaccgcctgg
     2401 actcgccggc gaagctccgg gagatcgccg cgtgggcgac gggaatcggc ccgagcaagc
     2461 tgatcttcga gaagctgccc gacctggccg cctgggcgca cgccgcgaag ctgacggtca
     2521 cgccgtggac cttcaggccg cctgcccccc cgcagttccc gacgattcgc gaagagatgt
     2581 cgtacttcct cttcaccctc ggcgtggatg ccctgttcac cgacaacccg gacatgttcc
     2641 cgaggacgaa atgactccgg cccggggcct gaacgccccg ggccccatcc tcatcccgga
     2701 tgcggcctga gtcccgccgg ctccggcgtc accagcgtgg cgggctcacc gagcccgggt
     2761 cggcttcccc acgaatatgt cgtagagcca gccccccagc acgccgcccg cgagcggccc
     2821 gacaatcggc acccaccacc aggagttccc ggcgcgaaag accccgcttc cccacccggc
     2881 catcgccgtg aagatcctcg ggccgaagtc gcgggcgggg ttgatggcgt agccgcagtt
     2941 cacgccgaag gcagcgccga tggcgacaac cagcaggccc acgatgaggg gcacgaggcc
     3001 tgcgggcggc gcgtggcggg gatcggtgat gccgaagagg acgcccatga ggatcgccgt
     3061 gccgaccact tgatcgatga atccaccggg gaatgtgctc aagtacggct gcgggtacgt
     3121 ggcgaagatg ccgcccgtgc cctgcgggcc cagcacctgg cggacgccgc cgtcgaaggc
     3181 ggccaaggcc tcgcggtacg tcaggtacac gacggcggcg gccacgaacg cgccgaggac
     3241 ctgggcggtc acgtacggca gcaccttgcg ccaggggaac ccgcggtgca cggccaccgc
     3301 gagggtcacc gccgggttca ggtgcgcgcc ggagacgccg atggacacgt agcagcccag
     3361 ggccacgccc aggccccagg tgaggttgat cgccaggtag ctgccgttga cctgcctgct
     3421 caagacgtac tgagcgacga cgcccgcgcc gaacagtatc aatacgaagg tcccgaagaa
     3481 ctcggccagc acttcccgcc acgtgtctcg cgccatgcgc ccgctcctgt ctcgagaaaa
     3541 gggtcccgac cggcgccaga gtataccgcc gaagtgtgct aaccttccga tgttttcggc
     3601 gccgtgggac ggcgccgccc ggggagcgat gccgtggcct acatcctcgc cctcgatcag
     3661 ggcacgacca gttcgcgcgc cattctcttc gatcgcagcg gcggcacctc cgccgtcgcc
     3721 cagcgcgaat tcgagcagca cttcccgacg cccggctggg tcgagcacga tccgcgcgag
     3781 atctgggcct cgcagatggc cgtcgccgcc gaggccctgg cgcgcgcgca ggcgggcccg
     3841 ggagacgtcg cggccatcgg catcacgaac cagcgggaga cgacggtggt ctgggaccgc
     3901 cagaccggcg agcccgtcta ccccgctatc gtgtggcagg atcggcgcac cgccgagcag
     3961 tgcgaccgcc tgaaggcgga cggcttcgag ccgctggtcc gcgagcggac cggcctggtc
     4021 atcgacgcgt acttctcggg cacgaaggtc gcctggatcc tcgaccacgt cgacggagcg
     4081 cgggcgaagg cggaagcggg ccgcctggcc ttcggcacga ttgacgcc
//
LOCUS       DAOSIF010000270         4085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19306_length_4085_cov_3.758065, whole genome shotgun sequence.
ACCESSION   DAOSIF010000270 DAOSIF010000000
VERSION     DAOSIF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19306_length_4085_cov_3.758065"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2832
                     /gene="purL"
                     /locus_tag="PLT35_09330"
                     /gene_synonym="purI"
     CDS             <1..2832
                     /gene="purL"
                     /locus_tag="PLT35_09330"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOG29467.1"
                     /translation="GPESRPDRIASPLSIMIDGPIGAASFNNEFGRPNLAGYFRTYEQ
                     TVAGVRRGYHKPIMLAGGVGNVWAAQALKAGFPAGALVLQIGGPALLIGMGGGAASSM
                     ATGANAPDLDFDSVQRGNAEMQRRAQEVIDRCRALGPRNPILSIHDVGAGGLSNAVPE
                     LVHGAGRGARIDLRAAPNEEPGMTPREVWCNEAQERYVLAVAPEDLGTFRALCERERC
                     PCAVLGHATADERLVVSDPVFGLDAVDMDLPALLGKPPRLTRDAVRLARPSPLFDLSG
                     LTIAGALDRVLRAPAVADKTFLVTIGDRTVGGLCARDQMAGPWQVPVADCAATLLSFE
                     GHAGEAFAIGERTPIAAIDAPASGRMAVAEALTNLAAAPVDSLSRVRLSANWMAAAGA
                     PGEDAALFDTVRAVALELCPALGISIPVGKDSMSMRTTWSEHGEPREVVSPVSLIVSA
                     FAPCADVRGTWTPQIRLDTGPSSLVLVDLARGRARLGGSILAQVHGQAGGEPPDLDDP
                     AGLRALFAALAELREARLVLAYHDRSDGGLIVTLLEMAFAGRAGLDIDADAIAPEPLG
                     DQALIAALFAEEAGAVIQVPGPYLGRAQLVLAGHGLEGRAVARPAIGGRVTIRRGSRV
                     LFGGDRIALQRAWSETTWLLQTLRDNPDCAQQEYDRILDAADPGLSPALTFDPAADVA
                     APFVAAGARPPIAILREQGVNGEIEMAAAFDRAGFEARDVHMSDIIAGRVSLAEFKAF
                     AACGGLSYGDVLGGGEGWAKSILYNARARDEFAAFFGRPDTFALGVCNGCQMMAALKD
                     LIPGAEAWPRFVKNASEQFEARLCTIEVLPGPSIFFAGMAGSRLPVATAHGEGRALFD
                     RPEDAASVLVAGRYVDNRGHPTEVYPLNPNGSAGGLTAVTTADGRFTAIMPHPERVFR
                     TVQLSWAPREWGEDSPWLRFFRNARVFAG"
     gene            complement(3462..>4085)
                     /locus_tag="PLT35_09335"
     CDS             complement(3462..>4085)
                     /locus_tag="PLT35_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29468.1"
                     /translation="AVSLAGMPAALLSAQDALALERGVREAIDVELAAAERPEAHINL
                     SNLYLKIGRVRDAEAELHTALRLDPRGIAARVNLADVFRAEGREAEAERVLREALGID
                     PGAAEAHHALGLLHIRLKRHDEALSHLGRAHRLRPDDARYGYVYAVGLDAVGRTERAV
                     QVLAALHKTRPGDADIIAALAAFEDRLGHPAAAAAWAEKLKAARGGR"
BASE COUNT          494 a         1581 c         1482 g          528 t
ORIGIN      
        1 ggccccgagt cgaggccgga ccggatcgcc tcgccgctct cgatcatgat cgacgggccg
       61 attggcgccg cctccttcaa caacgagttc ggccgcccga acctcgccgg gtacttccgc
      121 acgtacgagc agacggttgc cggggtgcgc cgcggctatc acaagcccat catgctggcc
      181 ggcggcgtcg gcaacgtctg ggcggcgcag gcgctgaagg ccggctttcc cgccggcgcg
      241 ctggtcctcc agatcggcgg gccggccctg ctcatcggca tgggcggcgg cgcggcctcg
      301 tcgatggcga ccggcgccaa cgcgccggac ctcgacttcg actcggtgca gcgcggcaac
      361 gccgagatgc agcggcgcgc gcaggaggtc atcgaccgct gccgcgccct cggcccgcgc
      421 aacccgatcc tctcgatcca cgacgtcggc gccggcggat tgtcgaacgc cgtgccggag
      481 ctcgtgcacg gcgccggccg cggcgcgcgc atcgacctgc gcgcggcgcc caacgaggag
      541 ccgggcatga cgccgcgcga ggtctggtgc aacgaggcgc aggagcgcta cgtcctggcc
      601 gtcgctccgg aggacctcgg gacgttccgc gcgctctgcg agcgggagcg ctgcccgtgc
      661 gccgtgctgg gccacgccac cgccgacgag cgcctcgtgg tcagcgaccc ggtgttcggc
      721 ctggacgccg tggacatgga cctgccggcg ctgctcggca agccgccgcg cctgacgcgg
      781 gacgccgtcc gcctggccag gccgtcgccg ctcttcgatc tgtcgggcct caccattgcc
      841 ggcgccctcg accgcgtgct gcgcgcgccc gccgtcgccg acaagacgtt cctcgtcacg
      901 attggcgatc ggaccgtggg cggcctgtgc gcgcgcgacc agatggccgg gccctggcag
      961 gtccccgtcg ccgactgcgc ggccacgctg ctgtcgttcg agggacacgc cggagaggcc
     1021 ttcgcgattg gcgagcgcac gccgattgcg gccatcgacg cccccgcgtc cggccgcatg
     1081 gcggtcgccg aggcgctgac gaacctcgcc gccgcgcccg tggactccct gtcgcgcgtc
     1141 aggctctcgg ccaactggat ggccgccgcc ggcgcgcccg gcgaggacgc ggcgctcttc
     1201 gacaccgtgc gcgccgtggc cctggagctc tgcccggccc tcggcatcag catccccgtc
     1261 ggcaaggact cgatgtccat gcgcacgacg tggagcgagc acggcgagcc gcgcgaggtc
     1321 gtgagccccg tctcgctcat cgtctcggcc ttcgcgccgt gcgccgacgt ccgcggcacg
     1381 tggacgccgc agatccgcct cgacaccggg ccctcgtcgc tcgtgcttgt cgatctggcg
     1441 cgcggccgcg cgaggctcgg cggatccatc ctggcccagg tccacggcca ggccggcggc
     1501 gagccgcccg acctggacga tcccgccggg ctgcgcgcgc tgttcgccgc gctggccgaa
     1561 ctgcgcgagg cccggctcgt gctcgcgtac cacgaccggt ccgacggcgg gctgatcgtg
     1621 acgctgctcg agatggcctt cgcgggccgc gcgggcctcg acatcgacgc cgacgcgatc
     1681 gcgcccgaac cgctcggcga ccaggctctc atcgccgcct tgttcgccga ggaagccggc
     1741 gcggtcatcc aggtgcccgg gccctatctc ggccgcgcac aactggtgct ggccgggcac
     1801 ggcctcgagg gccgcgccgt cgcccggccg gccatcggcg ggcgcgtcac catccggcgc
     1861 gggtcccggg tcctgttcgg cggcgaccgg atcgcgctgc agcgcgcatg gtcggaaacg
     1921 acgtggctgc tgcagacgct gcgcgacaac ccggactgcg cgcagcagga gtacgaccgg
     1981 atcctcgacg cggcggaccc cggcctgtcg ccggcgctga cgttcgatcc cgccgccgac
     2041 gtcgcggcgc cgttcgtcgc cgcgggcgcg cgcccgccca tcgccatcct gcgcgagcag
     2101 ggcgtgaacg gcgagatcga gatggccgcc gccttcgacc gcgcgggctt cgaggcccgc
     2161 gacgtccaca tgagcgacat catcgccggc cgcgtgtcgc tcgcggaatt caaggcgttc
     2221 gccgcctgcg gggggctctc gtacggcgac gtgctcggcg gcggcgaggg atgggcgaag
     2281 tcgatcctct acaacgcccg cgcgcgcgac gagttcgccg ccttcttcgg ccggcccgac
     2341 acgttcgccc tcggcgtctg caacggctgc cagatgatgg cggcgctgaa agatctgatc
     2401 ccgggagccg aggcctggcc ccggttcgtg aagaacgcgt cggagcagtt cgaggcgcgg
     2461 ctctgtacca tcgaggtgct gccgggcccg tcgatcttct tcgccggcat ggccggcagc
     2521 cggctgcccg tcgccacggc ccacggcgag gggcgtgccc tgttcgaccg gcccgaagac
     2581 gcggcgtccg tcctcgtggc cgggcggtac gtggacaacc gcggacaccc caccgaggtc
     2641 tatccgctca acccgaacgg ctcggccggc ggcctgaccg ccgtgacaac ggccgacggc
     2701 cgcttcaccg cgatcatgcc gcaccccgag cgcgtcttcc ggaccgtgca gctctcctgg
     2761 gcgccgcgcg agtggggcga ggacagcccc tggctgcgct tcttccgcaa cgcgcgcgtg
     2821 ttcgccggct gacggctccc ggggcctgaa gacccctagc tccatcggac gcacctgccg
     2881 gccagcgtga gcgggaccgc gcggcagcgc ccccgccgcc ggcaacccgc acgccggtag
     2941 ccgcggcgtc tccggagggc cggctcacgt ccgccggaag cccggcggcg gagcgtgctg
     3001 gcgagcgggc cctcgaacct ggccgcacgg cgtcagtccg caaaacggcg cgcaagggcc
     3061 gaagtccggg ctggggtctc gggggcccca gcctggcgcc gaccggaccc tgcagcgctg
     3121 atttgccggc cagcgtgagc gggaccgcgc ggcagcgccc ccgccgccgg caacccgcac
     3181 gccggtagcc gcggcgtctc cggagggccg gctcacgtcc gccggaagcc cggcggcgga
     3241 gcgtgctggc gagcgggccc tcgaacctgg ccgcacggcg tcagtccgca aaacggcgcg
     3301 caagggccga agtccgggct ggggtctcgg gggccccagc ctgccgccga ccggaccctg
     3361 aagcgctgat ttgccggcca gcgtgagcgg gaccgcgcgg cagcgcctcc gccgccggca
     3421 acccgcacac cggcagccgc ggcgtctccg gaaggcgagg cctatcgccc gccacgagcg
     3481 gctttgagct tttcggccca cgccgcggcg gccgccgggt gcccgagccg atcctcgaag
     3541 gcggcgagcg ccgcgatgat gtccgcgtcg cccggccggg tcttgtggag cgccgcgagc
     3601 acctgcaccg cgcgctcggt cctgccgacg gcgtcgaggc cgacggcgta cacgtacccg
     3661 taccgcgcat cgtccggcct caaccggtgc gcgcgcccga ggtgcgagag cgcctcgtcg
     3721 tgccgcttca gccggatgtg cagcaggccc agcgcgtggt gcgcctcggc ggcgcccggg
     3781 tcgatgccca gggcctccct cagaacgcgc tccgcctcgg cctcgcgtcc ttcggcgcga
     3841 aacacgtcgg ccaggttgac gcgcgcggcg atgccgcgcg ggtcgagccg aagggccgtg
     3901 tgaagctcgg cttccgcgtc gcggacgcgg ccgatcttca ggtacaggtt gctgagattg
     3961 atgtgcgcct ccggccgctc ggccgccgcc agctcgacgt cgatcgcctc gcgcacgccg
     4021 cgttcgagcg cgagggcgtc ctgcgccgag agcagcgcgg ccggcatccc tgccagcgac
     4081 accgc
//
LOCUS       DAOSIF010000271         4082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19328_length_4082_cov_3.992799, whole genome shotgun sequence.
ACCESSION   DAOSIF010000271 DAOSIF010000000
VERSION     DAOSIF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19328_length_4082_cov_3.992799"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLT35_09340"
     CDS             complement(<1..880)
                     /locus_tag="PLT35_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29469.1"
                     /translation="MPHEPAEFTVALQPRARFDAIDVRSRLQSAHGDVLAEFPRAYYV
                     SLHTTAGYLEQALAARLTRRAQNGVGLYVDAFRSVFPEDAGYEHDKLELRTELSEDQR
                     AVEPRNADSHLAFMAAGLRTCVSYRAGGGPVWFVDLDGVTNGGPRQRQTTVLAYNHEV
                     PVWRAQVTVPVSAHPMDSVNLRDPQIGFYQMLEEAIARYGVTKGRLRIELAPQERQAG
                     LTVNEYETLLMRHDLLEVLHNPFRFMIEKGRHAFADPRSIPAKTRDYAKYDIVRLINR
                     IVDSLGLNDSVIERKVA"
     gene            944..1582
                     /locus_tag="PLT35_09345"
     CDS             944..1582
                     /locus_tag="PLT35_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOG29470.1"
                     /translation="MSTPREWSFGEELAHSLTHGAGLVLGVAGLVFLVILAARTGDPW
                     RIAACTVYAVTLVLLYAASTLYHALSPTRARRVLQIVDHSAIFLLIAGTYTPFALVNL
                     RGPWGWALFAVVWSLAALGIAAKAVFGTRWPALSTAFYLGMGWTVLVALKPLVEHVAP
                     GGIAWLAAGGLAYTGGVAFYAWDRLRYGHAVWHVFVLAGSACHYVAVARYVV"
     gene            complement(1552..2052)
                     /locus_tag="PLT35_09350"
     CDS             complement(1552..2052)
                     /locus_tag="PLT35_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOG29471.1"
                     /translation="MKTVSRAGIFAAVGALGFALQTAVLWLLAGRAEMPVVPATLVAT
                     EAAVLHNFAWHVRWTWADRPAGPRALLGRLLRFNLANGGVSLAGGAVLMAILVHGCGV
                     HYLPANLASVLICSAVNFAASDGWVFRAEPQAARGEAGGLGGRRGSGGGGRPRAGQTT
                     YRATAT"
     gene            complement(2049..2756)
                     /locus_tag="PLT35_09355"
     CDS             complement(2049..2756)
                     /locus_tag="PLT35_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG29472.1"
                     /translation="MRASATTVETASARLAGHTRVHGSLLARAEKQALVWMASRLPAW
                     INSDHLTALGFASMAGVAASFVLARSSPGAGLPLVVLFLFLNWLGDSLDGTVARVRNK
                     QRPRYGYYVDHVVDIAGTSMMLAGLALSGYMHPLRAVSTLALWLLVAGESFLATNARR
                     VFRLSFMWLGPTELRILIAAAALSLLGGGMVPIPGLGPCRLFDVIGVAVSAGLIVAFT
                     VNVARNVSALYREETVR"
     gene            2919..>4082
                     /locus_tag="PLT35_09360"
     CDS             2919..>4082
                     /locus_tag="PLT35_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29473.1"
                     /translation="MTTRSSIRFGVHFRLRHAAAVAALAAAGIALGPARGPVRAHAAA
                     AAPASGAAAGQPAQDPNAVINPVMFQGLRFRSVGPHRGGRVTAVAGVRQQPCTFYMGA
                     TGGGVWKTTDCGSSWLPISDGQIDTGSIGAIAVSESNPDIVWVGTGSAAIRSNVIIGR
                     GVYKSTDAGRTWQYAGLKDAGQIGSIAIHPTNPDIVWLAAMGSPYGPTDRRGVFRTTN
                     GGREWARVLTVNRETGARVVAVNPANPNEVFAGMYRGFRKGWDIVSGGPADEGGIYKS
                     TDGGETWGHLRVGLPMKLIGKIDIDIARSKPSTIYAMVEAPGQEGGLYKSTDSGDTWA
                     LVNNSQRLRARPFYFHYVDVNPNDEHEVWVSEVGLWKSTDGGRTFSQVPTPHVD"
BASE COUNT          610 a         1484 c         1418 g          570 t
ORIGIN      
        1 gcgccacctt ccgctcgatg acgctgtcgt tcaggccgag cgagtccacg atccggttga
       61 tgagcctgac gatgtcgtac ttggcgtagt cgcgggtctt ggcgggaatc gagcggggat
      121 cggcgaaggc atgccggccc ttctcgatca tgaatctgaa cgggttgtgg agcacttcca
      181 gcaggtcgtg ccgcatcagg agcgtctcgt actcgttcac cgtgaggccc gcctgccgct
      241 cttgcggagc cagctcgatg cgcagccgtc ccttggtcac gccgtagcgc gcgatggcct
      301 cctcgagcat ctggtagaac ccgatctgcg gatcccgcag gttcaccgag tccatcgggt
      361 gcgcggacac cgggacggtg acctgcgccc gccagacggg cacctcgtgg ttgtaggcca
      421 gcaccgtcgt ctggcgctgc ctgggccctc cgttggtcac cccgtcgagg tccacgaacc
      481 acacgggccc gccgcccgcc cggtacgaca cgcacgtgcg cagccccgcc gccatgaacg
      541 cgaggtggga atcggcgttc cggggctcga cggcccgctg gtcctcgctc agctcggtgc
      601 gcagctccag cttgtcgtgc tcgtagccgg cgtcctcggg aaacaccgac cggaaggcgt
      661 ccacgtagag gccgacgccg ttctgggcgc ggcgcgtcag gcgggcggcc agggcctgct
      721 cgaggtagcc ggcggtcgta tggagggaga cgtagtacgc ccgggggaac tcggccagca
      781 catcgccgtg ggcggactgg agccggctcc tgacgtcgat tgcgtcaaac cgcgctcgag
      841 gttgcaaggc gaccgtgaat tccgccggtt cgtgcggcat agggcgcaac tatccataat
      901 acttcttcac cggctccgat gcacggaatt agactggaag gcgatgagca cccctcgcga
      961 gtggtcgttc ggcgaagagc tggcccacag cctcacgcac ggcgccggcc tggtcctggg
     1021 cgtcgcgggg ctggtcttcc tcgtcatcct ggccgcccgc accggcgacc cctggcggat
     1081 cgcggcctgc acggtctatg cggtcacgct ggtcctgctc tacgccgcct ccacgctcta
     1141 ccacgccctg tcgcccaccc gggccaggcg cgtgctccag atcgtcgacc actcggccat
     1201 cttcctcctg atcgccggca cctacacgcc cttcgccctc gtcaacctgc gcgggccctg
     1261 gggctgggcg ctgtttgccg tcgtctggtc gctggcggct ctcggcattg ccgccaaagc
     1321 ggtgttcggc acgcgctggc cggcgctctc gacggcgttc tatctcggca tgggctggac
     1381 ggtgctcgtc gccctgaagc cgctcgtcga gcacgtcgct cccggcggca tcgcctggct
     1441 ggcggcgggc ggcctggcct acaccggtgg cgtggccttc tatgcctggg accgcctccg
     1501 ctacggccac gccgtctggc acgtgttcgt cctcgccggc agcgcctgcc actacgtcgc
     1561 ggtagcccgg tacgtcgtct gaccggcgcg cgggcggcct cctccgccgg acccgcgccg
     1621 tcctcccagc ccgccagcct ctccccgcgc ggcctgcggc tcggcccgga acacccagcc
     1681 gtcgctggcc gcgaagttca cggcggagca gatgaggacg ctcgcgaggt tcgccggcag
     1741 gtagtgcacg ccacagccgt ggaccaggat ggccatcagc acggcgccgc ccgcgaggga
     1801 cacgccgccg ttcgccaggt tgaaccgcag gagccgcccg agaagcgccc tcggcccagc
     1861 cggccggtcc gcccaggtcc acctgacgtg ccacgcgaag ttgtgcagca cggccgcctc
     1921 ggtggccacg agcgtcgccg gcacgacggg catctccgcc ctcccggcca ggagccacag
     1981 gacggccgtc tgcagcgcga agccaagcgc gccgacggcg gcgaagatgc ccgctcgcga
     2041 gacggtcttc accggacggt ctcctcgcga tagagggcgc tcacgttgcg ggcgacgttg
     2101 accgtgaacg ccacgatcag gccggccgac acggccacgc cgatgacgtc gaacaggcgg
     2161 cacgggccga ggcccgggat gggcaccatc ccgccgccca gcagcgacag ggccgccgcg
     2221 gcaatcagga tgcggagctc cgtggggcca agccacatga aggacaggcg gaacaccctc
     2281 ctcgcgttcg tggcgagaaa cgactcgccg gccacgagga gccacagcgc cagggtggac
     2341 acggcccgca gcgggtgcat gtagccagag agggcgaggc ccgccagcat catcgacgtg
     2401 ccggcgatgt ccacgacgtg atccacgtag tatccgtaac gcgggcgctg tttgttccgg
     2461 acccgcgcca cggtaccgtc gaggctgtcg ccgagccagt tcaggaacag gaacagcacc
     2521 accaggggca gccccgcgcc gggggacgac cgcgccagga cgaacgacgc ggcgacgccg
     2581 gccatggacg caaagccgag ggccgtcagg tggtcggaat tgatccacgc gggcagtctc
     2641 gacgccatcc acacgagggc ctgcttctcg gcgcgcgcga gcaggctgcc gtggacgcgc
     2701 gtgtggccgg ccaggcgggc cgaagcggtt tcgacggtcg ttgctgacgc gcgcatcgga
     2761 tcctcctccg gcggcacatg cggccgccgt cacccgggaa tgcgcagacg gcgcccgaaa
     2821 cggctcacgc gggagcgcgc gaggccgggc gctccgcgtc gagccggagg ccgggctggt
     2881 atacttcgcg caactacggc cctgggggac ccgcgctgat gaccacgcgc tcatcgatcc
     2941 gcttcggcgt tcacttccgc ctgcgccatg ctgcggcggt cgcggccctt gcggccgcgg
     3001 ggatcgcgct gggcccggct cgcggccccg tgcgcgccca cgcggccgcc gcggcgccgg
     3061 catccggcgc cgcggcgggc cagcccgccc aggacccgaa cgccgtgatc aaccccgtga
     3121 tgttccaggg gttgcgcttt cgctccgtcg gaccgcaccg cggcggccgc gtcaccgccg
     3181 ttgccggcgt gcgccagcag ccgtgcacgt tctacatggg cgccacgggc ggcggcgtct
     3241 ggaagacgac cgactgcggc tcgtcatggc tgcccatcag cgacggccag atcgacaccg
     3301 gctcgatcgg cgccatcgcc gtgtccgaat cgaacccgga catcgtctgg gtggggaccg
     3361 ggagcgcggc catccggagc aacgtcatca tcggacgcgg cgtctacaag tccaccgacg
     3421 cgggccgcac ctggcagtac gccgggctga aagacgccgg ccagatcggc tccatcgcca
     3481 tccacccgac caacccggac atcgtctggc tggcggcgat gggctcgccc tacgggccga
     3541 ccgaccggcg gggcgtcttc aggacgacga acggcgggcg ggagtgggcg cgcgtcctga
     3601 cggtcaaccg cgagaccggc gcgcgcgtcg tcgcggtcaa cccggcgaac ccgaacgagg
     3661 tcttcgccgg catgtaccgc ggcttccgca agggctggga catcgtgagc ggcgggccgg
     3721 ccgacgaagg cggcatctac aagtccaccg acggcggcga gacctggggc cacctgcgcg
     3781 tcgggctccc gatgaagctc atcggcaaga tcgacatcga catcgcgcgc agcaagccga
     3841 gcacgatcta cgccatggtg gaagcgcccg gccaggaagg cgggctctac aagtccaccg
     3901 acagcggcga cacgtgggcg ctcgtcaaca acagccagcg gctgcgcgcc cggccgttct
     3961 acttccacta cgtggacgtc aacccgaacg acgagcacga ggtgtgggtg agcgaggtcg
     4021 ggctctggaa gtccaccgac ggcggccgca cgttctcgca ggtgccgacg ccgcacgtcg
     4081 ac
//
LOCUS       DAOSIF010000272         4079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19347_length_4079_cov_3.870030, whole genome shotgun sequence.
ACCESSION   DAOSIF010000272 DAOSIF010000000
VERSION     DAOSIF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19347_length_4079_cov_3.870030"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1877
                     /locus_tag="PLT35_09365"
     CDS             72..1877
                     /locus_tag="PLT35_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29474.1"
                     /translation="MGHVSPPAARAAIATALTRDTLVLLFYAVAVTEALIYSLPLMDP
                     GTLTAFGASPFLIPFVATATAAGFYGLGRVQEREERLFWSHLAYACVFWLATLAAIAA
                     VPVAQWRLIDDVLVDATYLFFYSPILFAAECKPHIPGVGPHRDVERQLRWAGVTLLVL
                     GWFAYFVVTPAVLDPDRFGATASSAFLFMTLDVTIVVRFTWRAWTSGSTRWRVLYGTV
                     ALTGVALFATDTLDVLEGLRVFALADGALTDLLWAIPAFVLLLAFRLRDADLPRAIDP
                     ATHERAVSAGLEPVRVGSFLVGSALAFPLVHFAFHTAYPVLPALVATHRVIVTAELVL
                     LGALGVIAFRHLEHNRVLAERRRAALEERVRRARTLEAVSRFAGVVAGEYSALVRVLG
                     AFVDRAIDATGPGDPLHDEAIRAAEQVQRAADFTGNLQAISRQQRGHPVRVDVAAAVS
                     ERAHELRQILGPQILLEIAPSRGPCVAEIDPAHLRAMLLDLVTNARDAMPRGGRCRIE
                     TGAVDLDGDAAMGMGIHAGRFARLAVRDTGPGIPSEILPHIFEPFFSSKPGEGRGAGL
                     GLATLYALASQYGGCVTAASGPGDTAFEILLPSAG"
     gene            complement(1866..3167)
                     /locus_tag="PLT35_09370"
     CDS             complement(1866..3167)
                     /locus_tag="PLT35_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG29475.1"
                     /translation="MDAIGFEVLVIAVLLVANGVFAMAEIAVVAARKSRLRHWAERGD
                     ARARAALDLASSPDRFLAAVQVGISAVGILAGAFGGATIAGAIAARLQGVPRLAPYGD
                     AIGLAVVVTVITYCSLVIGELVPKRLALNHPERLASYLAGPMRRLSAAAAPVVWLLEA
                     STRAVLWLLRAKPSKDPVITPEELKLIISQGTEGGVFEAAEQEMLEGVLHLADRTVGA
                     LMTPRTRMVSVDVDDPPDAILRKIAASHRSRFPVVQHDEENVIGILRSKDLLVRVMAG
                     EPFDLRPLLQPVVFVPESMTAPRVLETFKQQGAHIAIVTDEFGSVQGLVTQTDILESI
                     VGELPARGEGDPPSAVRRDDGTWLVDGLLDIPALREAIALPELPGEHEGVYQTVGGFI
                     THLFGRIPAAGQHATWRDWRFEVVDMDGHRVDKVLVGPLAR"
     gene            complement(3330..>4079)
                     /locus_tag="PLT35_09375"
     CDS             complement(3330..>4079)
                     /locus_tag="PLT35_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG29476.1"
                     /translation="VHRKGLALDGKNPALLYGYGGFNITTAPGFSALRVALLEQGFVY
                     ASANLRGGGEYGEAWHRAGMKLNKQNVFDDCIAAAEWLIANKYTSPAKLAVQGGSNGG
                     LLVGAVMNQRPELFRAAVPQVGVMDMLRFHKFTIGWNWIADYGSSDNPDEFKALYAYS
                     PLHNIRAGVKYPATLITTADHDDRVVPAHSFKYAATLQEKASRETPILIRIETKSGHG
                     ASSTAKQLETTADIYAFLFRELGVTPKLAAK"
BASE COUNT          501 a         1509 c         1430 g          639 t
ORIGIN      
        1 tcttgcatgc tttggcgccg ggacctcaag gacgtcccgt ctcgggccga taagtccggc
       61 tgttgccgcc catgggacac gtgtctccgc cagctgctcg ggccgcgatc gccaccgcgc
      121 tgacgcgcga cacgctcgtc ctgctcttct acgccgtcgc cgtcacagag gcgctcatct
      181 attcgctgcc gctgatggac ccgggcacgc tgaccgcctt cggcgcgtcg ccgttcctga
      241 tccccttcgt cgccacggcg acggccgccg gcttctacgg gctgggccgc gtccaggagc
      301 gggaggagcg gctgttctgg tcgcacctcg cctacgcctg cgtcttctgg ctggccacgc
      361 tcgcggccat cgccgccgtg ccggtcgccc agtggcggct catcgacgac gtgctggtcg
      421 acgcgacgta cttgttcttc tactcgccga tcctgtttgc cgccgagtgc aagccgcaca
      481 tcccgggcgt gggtccgcac cgggacgtgg agcgtcagct gcgctgggcc ggcgtgacgc
      541 tgctcgtgct cggctggttc gcctacttcg tcgtgacgcc ggccgtcctg gatccggacc
      601 gcttcggcgc cacggcctcc tcggcgttcc tgttcatgac gctggacgtc acgattgtcg
      661 tccggttcac ctggcgggcg tggacgtcgg gttccacgcg gtggcgcgtg ctctacggca
      721 ccgtcgcgct gacgggcgtc gccctgttcg ccaccgacac gctggacgtc ctcgaggggc
      781 tgcgcgtgtt cgcgctggcg gacggcgcgc tgacggacct cctgtgggcc attcccgcgt
      841 tcgttctgct gctggctttc cggctgcgcg acgccgatct gccccgggcg atcgatcccg
      901 caacgcacga gcgcgccgtg agcgccggcc tcgagcccgt gcgggtgggc agcttcctgg
      961 tgggcagcgc cctcgccttt ccgctggtcc acttcgcgtt ccacacggcg tatcccgtcc
     1021 tgcccgccct cgtcgccacc caccgggtga ttgtgaccgc ggagctcgtt ctgctgggcg
     1081 cgctcggcgt catcgcgttc cggcacctag aacacaaccg ggtcctggct gagcggcggc
     1141 gcgcggccct cgaggagcgg gtccgccgcg cgcggacgct cgaggcggtg agccgcttcg
     1201 ccggcgtcgt ggccggcgag tactcggcgc tcgtccgcgt cctcggcgcg ttcgtggacc
     1261 gcgccatcga cgccaccggc cccggcgatc cgctgcacga cgaggccatc cgcgcggccg
     1321 aacaggtgca gagggccgcg gacttcaccg ggaacctgca ggccatcagc cgccagcagc
     1381 gcgggcaccc ggtgcgcgtc gacgtggccg cggccgtgtc ggagcgcgcg cacgagctgc
     1441 gccagattct cgggccgcag atcctgctgg agatcgcgcc gtcgcggggg ccgtgcgtcg
     1501 ccgagatcga cccggcgcac ctgcgcgcca tgctgctcga cctcgtgacc aacgcccgcg
     1561 acgcgatgcc ccgcggcggc cgctgccgca tcgagaccgg cgccgtcgat ctcgacggcg
     1621 acgcggcgat gggcatgggg atccacgccg gccgtttcgc ccggctcgcc gtgcgcgaca
     1681 ccgggccggg catcccgagc gagatcctgc cgcacatctt cgagcccttc ttctcgtcga
     1741 agccgggcga ggggcggggg gcggggctgg ggctcgccac gctctacgcg ctcgccagcc
     1801 agtacggcgg ctgcgtgacg gctgcaagcg ggccgggcga caccgccttc gagatcctgc
     1861 tgccgtcagc gggctagggg ccccaccagc accttgtcca cgcggtggcc gtccatgtcc
     1921 accacctcga accgccagtc cctccaggtg gcgtgctggc ctgcggcggg gatgcggccg
     1981 aacaggtggg tgatgaagcc gccgacggtc tggtagacgc cctcgtgctc gcccggcagt
     2041 tcgggcaggg cgatggcctc ccgcagcgcg ggaatgtcga gcaggccgtc caccagccac
     2101 gtgccgtcgt cgcggcgcac ggccgagggc ggatccccct cgccccgggc gggcagctcc
     2161 ccgacgatcg actccaggat gtcggtctgc gtgaccagcc cctgcacgct gccgaactcg
     2221 tcggtgacga tggcgatgtg ggcgccctgc tgcttgaacg tctcgagcac ccgcggcgcc
     2281 gtcatgctct cgggcacgaa caccaccggc tgcagcagcg gacggaggtc gaagggctcg
     2341 ccggccatca cgcgcaccag caggtccttc gagcgcagga tgccgatgac gttctcctcg
     2401 tcgtgctgca ccaccgggaa gcgcgatcgg tggctcgccg cgatcttgcg caggatcgcg
     2461 tccggcgggt cgtccacgtc caccgacacc atgcgggtcc gcggcgtcat gagggcgccc
     2521 accgtccggt cggcgaggtg caggacgccc tcgagcatct cctgctcggc cgcctcgaac
     2581 acgccgccct cggtgccctg gctgatgatc agcttcagct cctccggcgt gatgaccgga
     2641 tccttcgacg gcttcgcccg cagcagccag agcaccgcgc gcgtggacgc ctcgagcagc
     2701 cagaccaccg gcgccgcggc ggccgagagc ctccgcatcg ggccggcgag gtacgaggcg
     2761 aggcgctccg ggtggttgag ggcgaggcgc ttcggcacta gctcgccgat cacgagggag
     2821 cagtacgtga tgaccgtgac gacgacggcg aggccgatgg cgtcgccgta gggcgccagg
     2881 cgcggcacgc cctgcagccg cgcggcgatc gccccggcga ttgtcgcgcc gccgaaggcg
     2941 cccgccagga tgccgacggc cgagatgccg acctgcacgg ccgcgaggaa gcggtcagga
     3001 ctcgacgcca ggtcgagcgc ggcgcgggcg cgggcgtcgc cgcgctcggc ccagtgccgc
     3061 agcctgctct tacgggcggc caccaccgcg atctcggcca tcgcgaacac cccgttcgcg
     3121 acgagcagga ccgcgatgac gaggacttcg aagccgatcg cgtccaccgg ggcattctat
     3181 cgcgacagcc gggcaggtgc agcggcgcgg gcgcgtcact cggctgccgc gcgcgggcgc
     3241 cgtcgaggcg cgcgcgtgcg cccccacgcc gtggtcggcg tgacggccgg gcgcgccgcc
     3301 ccctgtcctt cctgcctggg cccctgccgc tacttcgccg ccagcttcgg cgtcaccccc
     3361 agctcgcgga acagaaacgc gtagatgtcg gccgtcgtct cgagctgctt ggcagtgctg
     3421 ctcgcgccgt gccccgactt ggtctcgatc cggatcagga tcggggtctc gcggctcgcc
     3481 ttctcctgca gcgtggccgc gtacttgaac gagtgcgccg gcaccacgcg gtcgtcgtgg
     3541 tcggccgtcg tgatgagggt ggccgggtac ttcactccgg ccctgatgtt gtggagcggc
     3601 gagtaggcgt agagcgcctt gaactcgtcc gggttgtcgc tcgagccgta gtcggcgatc
     3661 cagttccagc cgatcgtgaa cttgtggaac cgcagcatgt ccatgacgcc cacctgcgga
     3721 acggccgccc ggaacagctc cggccgctgg ttcatgacgg cgccgacgag caggccgccg
     3781 ttcgatccgc cctggacagc cagcttcgcg ggtgaggtgt acttgttcgc gatcagccac
     3841 tcggcggccg cgatgcagtc gtcgaacacg ttctgcttgt tcagcttcat cccggcgcgg
     3901 tgccaggcct cgccgtactc gccgccgccc cgcaggttcg cggaggcgta cacgaagccc
     3961 tgctcgagga gggccacgcg cagcgcgctg aagcccggcg ccgtcgtgat gttgaacccg
     4021 ccgtacccgt agagcagcgc ggggttcttc ccgtcgagcg ccagcccctt ccggtgcac
//
LOCUS       DAOSIF010000273         4053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19504_length_4053_cov_4.224112, whole genome shotgun sequence.
ACCESSION   DAOSIF010000273 DAOSIF010000000
VERSION     DAOSIF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19504_length_4053_cov_4.224112"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLT35_09380"
     CDS             <1..259
                     /locus_tag="PLT35_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOG29477.1"
                     /translation="DEAGVRVTILCFARLRDLVGSPRLLRDVPDGATVETVWAALAAE
                     FPAVAPHRPAVAAAVNARFARFASPVAEDDEVAFLPPVAGG"
     gene            340..1416
                     /gene="prfA"
                     /locus_tag="PLT35_09385"
     CDS             340..1416
                     /gene="prfA"
                     /locus_tag="PLT35_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG29478.1"
                     /translation="MLDKLMALEKKYDELLSLLGDASVQADPPQYRTHAKALSEIEPV
                     VAKFREYKAVVSEIAQAEELARGPELDLQALAQEELPGLKAKAEALIAEITTLLTPRD
                     PNDEKNVVLEIRAGTGGDEAALFAADLFRMYARYAERQGWRVDVLSSNENGVGGLKEV
                     IATVEGRRVFGRLKFESGVHRVQRVPATEASGRIHTSTATVAVLPEAEEVDIHIDPKD
                     LRIDTFCSSGPGGQSVNTTYSAVRLTHIPTGLVVSQQDEKSQIKNKAKALKVLRARLY
                     EMEMRKQQDAIAKDRKSQVGTGERSEKIRTYNFPQNRITDHRIGFTTHRLADVLDGDV
                     GELVDETVAFFTAERLNETSARGG"
     gene            1520..2578
                     /gene="prmC"
                     /locus_tag="PLT35_09390"
     CDS             1520..2578
                     /gene="prmC"
                     /locus_tag="PLT35_09390"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOG29479.1"
                     /translation="MADSAILQQRILAARHRFERAGIGRAEAALDADVLARHALGGWE
                     RGRLLASMREACPPGFDEAFEPLVARREHREPSGYITGVREFWGIDIEVGRGVLVPRP
                     ETELLVDEALARLSAPAEASRRAGRAMVDAFPSVSIADVGTGSGCVAVALARWLPGAA
                     VAAIDASDEALAYARRNAARHGVQDRVLVVRGDLLGAQAGPFDAVVSNPPYVPTEDIA
                     GLPPEIRGYEPTGALDGGPDGLAVIRRLVPEAAARLRPGGWLLFEFGFGQAEGVRAIV
                     EAEPAFEWHGTRADLAGIPRVAMARRRVTAAGGPPPDPESPPPTHGLPACPEQAEEPA
                     SARQAESPAPSPEPRAPD"
     gene            2591..2932
                     /locus_tag="PLT35_09395"
     CDS             2591..2932
                     /locus_tag="PLT35_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOG29480.1"
                     /translation="MPCLFCRIAAGEVPARTVHEDEALIAFHDIHPRAPVHVLVVPRR
                     HISTLNELAPEDDALVGAMTRRAAAIARQLGIAERGYRTIFNCNRDAGQTVFHIHLHL
                     LGGWSSANPFE"
     gene            complement(2969..3358)
                     /locus_tag="PLT35_09400"
     CDS             complement(2969..3358)
                     /locus_tag="PLT35_09400"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG29481.1"
                     /translation="MIITFPGGARVDAEYGPYTIRTDQSPQGGGEGSAPAPFTLFLAS
                     VGTCAGIYVLSFCKQRGLPIDGIRIVQRTERDPRTGLVAKVGLDIQVPPGFPAKYHDA
                     LVRAADQCAVKKHMEHPPAFDVRTVVA"
     gene            complement(3436..>4053)
                     /locus_tag="PLT35_09405"
     CDS             complement(3436..>4053)
                     /locus_tag="PLT35_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29482.1"
                     /translation="RPAQWSHMLAVRPACRAAGVARALKLEQRRACLAQNVDLVEWSF
                     DPLAAVNAHLNLRRLGATVREYAEDLYGASDSPLHRGAPTDRFIAEWRLRSPRVETAL
                     AAGSGPRRDGALQAFPGVVRVNATEPRGAWQACAGWDMSRGDDELAVAVPGRYQEMLA
                     SEPALAREWRAASREMFAGYLGRGYQAVDFAADGVGGGTYLLRRA"
BASE COUNT          606 a         1419 c         1480 g          548 t
ORIGIN      
        1 cgatgaggcg ggcgtgcggg tgacgatcct gtgcttcgcc cgcctgcggg acctggtggg
       61 atcgccgcgg ctgcttcgag atgtgccgga tggcgcgacg gtcgagaccg tatgggccgc
      121 gctcgccgcc gagttcccgg ccgtggcccc gcaccggccg gctgtcgcgg ccgcggtgaa
      181 cgcgcggttc gcgcggttcg catcacccgt ggccgaggac gacgaggtgg cgttcctccc
      241 gccggtggcg gggggatagc tggaatccgg cagtcggcat gcggaagccg gacaacgtcc
      301 ggcgcggccc gctcgggcct tgccggcaag gacgagacca tgctcgacaa actgatggcg
      361 ctcgagaaga agtacgacga gctcctgtcg ctgctgggcg acgcgtcagt ccaggcggac
      421 ccgccgcagt accgcaccca cgccaaggcg ctttccgaga tcgagcccgt cgtcgcgaag
      481 ttccgcgagt acaaggccgt cgtgtcggag atcgcccagg ccgaggaact ggcgcgcggc
      541 ccggagctgg acctgcaggc gctggcgcag gaggagctgc cgggcctgaa ggcgaaggcc
      601 gaggcgctca tcgcggaaat cacgacgctg ctcaccccga gggacccaaa cgacgagaag
      661 aacgtcgtgc tggaaatccg cgccggcacc ggcggcgacg aggcggcgct cttcgcggcg
      721 gacttgttcc ggatgtacgc gcgctacgcg gagcggcagg gctggcgcgt cgacgtgctc
      781 tcgagcaacg agaacggcgt cggtggcctg aaagaggtca tcgcgacggt cgaaggcagg
      841 cgcgtctttg gccggctgaa gttcgagagc ggcgtgcacc gggtgcagcg cgtgccggcc
      901 accgaggcga gcgggcgcat ccatacctcg acggccaccg tcgccgtcct tcccgaagcc
      961 gaggaggtgg acattcacat cgacccgaag gacctgcgca tcgacacgtt ctgctcgagc
     1021 ggcccgggag gccagagcgt gaacaccact tactcggccg ttcgcctcac gcacatcccc
     1081 acgggcctcg tcgtgtcgca gcaggacgag aagtcgcaga tcaagaacaa ggccaaggcg
     1141 ctgaaggtcc tgcgcgcgcg cctctacgag atggagatgc ggaagcagca ggacgcgatt
     1201 gccaaggacc gcaagagcca agtcggaacg ggcgagcgct cggaaaagat ccggacctac
     1261 aacttcccgc agaaccgcat cacagaccac cggatcggct tcacgacgca ccgcctggcc
     1321 gacgtcctcg atggcgacgt gggcgaactc gtcgacgaga ccgtcgcgtt cttcaccgcc
     1381 gagcgcctca acgagaccag cgcgcgcgga ggatagggcc cggggcccgt tcccggggcc
     1441 cggagcgcgg gacgcggggc acggacaccg cgtgccgggc tccatgccgc atactggaag
     1501 ccagcgtcag gaatccgcca tggcagactc ggcaatcctc cagcagcgca ttctggcggc
     1561 gcggcacagg tttgagcgcg cgggcatcgg ccgcgcggag gccgcgcttg acgccgatgt
     1621 gctggcccgc cacgcgctgg gcgggtggga gcgcggccgt ctgctggcgt cgatgcggga
     1681 ggcctgcccg cctgggttcg acgaggcgtt cgaaccgctc gtcgcccggc gcgagcatcg
     1741 cgagccctcc ggctacatca ccggcgtccg cgagttctgg ggcatcgaca tcgaggttgg
     1801 ccgcggggtg ctcgtgccgc gccctgagac cgagctgctc gtcgacgaag cgctggcccg
     1861 cctgagcgcg cccgccgagg cgtctcgccg cgcaggccgg gccatggtgg atgcctttcc
     1921 gagcgtatca atcgcggatg tcgggaccgg ctcgggctgt gtggcggtcg cgctggcgcg
     1981 gtggctcccc ggggccgcag tcgccgccat cgacgcgtcg gacgaggcgc tggcgtacgc
     2041 ccgccggaac gcggcgcgcc atggcgtcca ggaccgcgtc ctcgtggtgc gcggcgatct
     2101 gctcggagcc caggctgggc cgttcgacgc cgtggtgtcg aacccgccct acgtgcccac
     2161 ggaagacatc gccggcctgc cgcccgagat ccgcggctac gagccgacgg gcgcgcttga
     2221 cggcgggccg gacggcctgg ccgtcatccg ccgcctggtt cccgaggctg cagccaggct
     2281 gcgtccgggc ggctggctcc tgttcgaatt cgggttcggc caggcggagg gcgtgcgcgc
     2341 gatcgtcgag gcggaaccgg cgttcgagtg gcacgggaca cgcgccgacc tcgccggcat
     2401 cccccgtgtc gccatggcca gaagacgggt cactgcggct ggcggtccgc ccccggatcc
     2461 cgagtctccg cctccaacac acggcttgcc ggcttgccct gaacaagccg aagagccggc
     2521 ttcggcgcgg caagccgagt ccccggcccc gagtcccgag ccccgagctc cggactagaa
     2581 taggcatccg atgccctgcc tcttctgccg catcgccgcc ggcgaggtgc ccgccagaac
     2641 agtgcacgag gacgaggccc tcattgcgtt ccacgacatc cacccgcgcg cgccggtgca
     2701 cgtgctggtc gttccgcgcc gccacatctc gacgctcaac gagctcgctc cggaagacga
     2761 tgcgctcgtc ggcgcgatga cgaggcgcgc cgcggccatc gcccggcagc tcggcattgc
     2821 cgaacggggc taccggacca tcttcaactg caaccgggac gcaggccaga cggtgttcca
     2881 catccacctg catctgcttg gcgggtggtc ctccgccaac ccgttcgagt agaccgcggc
     2941 tccgcgcctc gggtgcagcg ccgcccggct acgccaccac tgtccgaacg tcgaaggcgg
     3001 gcggatgctc catgtgcttc ttgacggcgc actggtctgc ggcccgcacc agggcgtcgt
     3061 ggtacttcgc cgggaagccg ggcgggacct ggatgtcgag gccgaccttc gcgacgaggc
     3121 ccgtccgcgg atcgcgctcc gtccgctgga cgatgcggat cccgtcgatc gggaggccgc
     3181 gctgcttgca gaaggacagg acgtagatgc ccgcgcaggt gccaacggag gccaggaaca
     3241 gcgtgaacgg agccggggcg gatccctccc cgcctccctg gggggactgg tcggtgcgga
     3301 tcgtataggg accgtactcg gcgtcgacgc gcgcgccgcc gggaaacgtg atgatcatgt
     3361 ccatggcttg ttggatgcgg gtgcgcggga attggtgacg gccggcggag ctgccgccgg
     3421 cgctcccctg gggcatcacg cgcgtcgaag caggtaggtc ccgccgccga cgccatcggc
     3481 ggcgaaatcg acggcctggt agccccggcc gaggtacccg gcgaacatct cgcgggacgc
     3541 cgcgcgccac tcgcgcgcga gggcgggctc gctcgcgagc atctcctggt atctcccggg
     3601 cacggccacc gccagctcgt cgtctccgcg cgacatgtcc cagccggcgc acgcctgcca
     3661 agcgccgcga ggctcggtgg cgttcacgcg gacgacgccg ggaaacgcct ggagggcgcc
     3721 atcgcgccgg gggccggagc cggccgccag cgcggtctcg acgcgcggcg agcggagtct
     3781 ccactcggcg atgaacctgt cggttggcgc gccgcggtgc agggggctgt cggacgcgcc
     3841 gtagaggtcc tccgcgtact cccggaccgt ggcgccgagg cggcgcaggt tgaggtgggc
     3901 gttgaccgcc gccagcggat cgaagctcca ttccacgaga tcgacgttct gcgccaggca
     3961 ggcgcgccgc tgctccagct tcaaggctcg cgcgacgccg gctgcacggc aggccggccg
     4021 gacggcgagc atgtgcgacc actgggccgg gcg
//
LOCUS       DAOSIF010000274         4051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19518_length_4051_cov_4.268519, whole genome shotgun sequence.
ACCESSION   DAOSIF010000274 DAOSIF010000000
VERSION     DAOSIF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19518_length_4051_cov_4.268519"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLT35_09410"
     CDS             <1..727
                     /locus_tag="PLT35_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29483.1"
                     /translation="FPYPTLTLLYDEFAAEHLARAGRIPPRALAVTGSSRLDALVERA
                     AAITPDDMERTRRAVGAGAGDALVVVAAKFSQIGPVFEQLVREAGEIPGVRLVVKPHP
                     AEAAAPYLAAGGGAPHLTIAPAREGLADLIRVSRLLVTVNSTAAIEAMVMGVPSLVLG
                     LPNNLTPFVEAGAMSGVEEGEPIGPALRALLADEGGRRAVLERSAAFMRRYRVESDGR
                     AALRAAERIAGLAAARGGPGEVS"
     gene            745..1707
                     /locus_tag="PLT35_09415"
     CDS             745..1707
                     /locus_tag="PLT35_09415"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG29484.1"
                     /translation="MRALITGGAGFIGSHLAETLLGGGHSVSVIDDLSTGSFDNIAHL
                     KGRAGFSYTIDTIMNEPVLAELVDQCDVIYHLAAAVGVRLIVEAPVRTIETNVHGTEV
                     VLKHASKKQRTVVVASTSEVYGKSDDVPFREDGDIALGPTVKHRWAYACSKAMDEFLA
                     LAYWKEKRLPVIIVRLFNTVGPRQTGRYGMVVPSFVRQALAGEPLTVYGDGTQSRCFT
                     WVGDVVGALAALAGRREALGRVYNLGSTEEVSIRALAERVKALTGSASPIVSVPYDQA
                     YESGFEDMPRRVPDITRIRALIGFEPKASLDEILQLVIDHHRTR"
     gene            1711..2664
                     /locus_tag="PLT35_09420"
     CDS             1711..2664
                     /locus_tag="PLT35_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOG29485.1"
                     /translation="MPSTLRRRARRATESLLLRHASLVRRLLLAAGRDRAPRPPEIVQ
                     IEATNICNAKCTFCPRDDMTRRQGIMDWALYQKIVDECAALGIGHVRMHNYGEAFVDR
                     KLPEKIAYAKSKGIREVGVITNGSLLGPDAARAIVLAGLDAINISLDAAGKETFESTR
                     VGLKYDRVVANVEGLIRIRRELGRTRPKLILSFVRQDNSAEEQAFIDHWSAVADKIHV
                     TNLHNWAGALTRRGEVNFPCYRQWLTFTVLWDGRVSLCCADLDGRVVLGDLNTQTIAE
                     IWNGEAYRRVRREQLQSGGPAICRNCDLPAKDSPLWVARLR"
     gene            2833..>4051
                     /locus_tag="PLT35_09425"
     CDS             2833..>4051
                     /locus_tag="PLT35_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29486.1"
                     /translation="MRIRTRAAAALFAAGVSVTGTAWAQALPAGPSVQQYAGFTRWPA
                     VAYDSEHGVYLAVWGNGSIRGRFINDDGTAASAWFQISDAAPNAQTPRVAFSPDTGQF
                     LVVWHSSDGVAGNPEGTRVRGRLVDFSSAGPVGPTQVYSNANYTTRWEVGPAVAYATG
                     SQQFLVAYNIFAATGAEIGARLIDTTGAPVGPEIAVTSSSGEYDREPAVGYFPSTNAF
                     VVAWSGGSDSSPDFIRTRMFGAADGAAIGPAVTVAQSTFTYVPEVAYNLATGQMLVTW
                     IQSDPAGGGWRPFRNFLDDAGVVVGTNLRLSSTVGAYDANSVAYNAPAMTFFLVTHGN
                     TEQDVGFEITGDGMPVSPAMVVTSVTAPDLNGSYNPRIAASAQTPSWLIGTAASFSSL
                     WTQLITGVDLGGGG"
BASE COUNT          614 a         1417 c         1429 g          591 t
ORIGIN      
        1 attcccgtat cccacgctca cgctgctcta cgacgagttc gcggcggaac accttgcgcg
       61 cgcaggccgg atcccgcctc gggcgctggc ggtcacgggc agctcgcgcc tcgacgccct
      121 ggtggagcgg gctgccgcga tcacgccgga cgacatggag cggacgcgcc gtgcggtcgg
      181 cgcgggcgcc ggcgacgctc ttgtcgtggt ggcggccaag ttctcgcaga ttgggccggt
      241 gttcgagcag ctggtccgcg aggccggcga aatccccggc gtgcgcctgg tcgtcaagcc
      301 gcatcccgcc gaagccgccg cgccctacct ggccgccggc ggcggggccc cgcacctgac
      361 gatcgcgcct gcgcgcgagg gcctcgccga tctgatccgc gtcagccgcc tgctggtgac
      421 ggtcaactcc acggccgcca tcgaagcgat ggtgatgggc gtgcccagcc tcgtcctggg
      481 gctgccgaac aacctgacgc ccttcgtcga ggcaggggcc atgtcggggg tcgaagaggg
      541 cgagccgatc ggaccggccc tccgggcgct gctggccgac gagggcgggc ggcgggccgt
      601 cctcgagcgg agcgcggcct tcatgcggcg ctaccgcgtg gaatccgacg gccgagccgc
      661 cctgcgggcg gcggagcgca tcgccggcct cgcggcggcg cggggcggcc ccggcgaggt
      721 atcctagaaa gtcaaggagc atcgatgcgc gcgttgatca ccggcggcgc cggtttcatc
      781 ggatcccatc tcgccgagac gctgctcggg ggagggcact ccgtatccgt catcgacgac
      841 ctctcgacgg ggtctttcga caacatcgcc cacctgaagg gccgggcggg gttctcgtac
      901 acgatcgaca ccatcatgaa cgagcccgtg ctggccgaac tcgtcgacca gtgcgacgtc
      961 atctaccacc tcgccgccgc cgttggcgtg cggctgattg tcgaagcccc cgtccggacg
     1021 atcgagacga acgtgcacgg gacggaggtc gtcctgaagc acgcgtccaa gaagcagcgc
     1081 acggtggtgg tcgcctcgac ctccgaggtc tacggcaaga gcgacgacgt gccgttccgg
     1141 gaggatggcg acatcgccct cggcccgacg gtcaagcacc ggtgggcgta cgcgtgcagc
     1201 aaggccatgg acgagttcct ggccctcgcg tactggaaag agaagcgcct cccggtcatc
     1261 atcgtccggc tgttcaacac cgtgggcccg cgccagaccg gccgctacgg gatggtcgtc
     1321 cctagcttcg tccgccaggc gctcgcaggg gagcccctca ccgtgtacgg cgacggcacg
     1381 cagagccgct gcttcacctg ggtcggcgac gtggtgggcg cgctcgcggc cctggccggg
     1441 cgccgggagg cgctcgggcg ggtctacaac ctcgggagca ccgaggaggt gtcgattcgc
     1501 gcgctggccg agcgcgtgaa ggccctcacc ggcagcgcct cgcccatcgt cagcgtcccc
     1561 tacgaccagg cctacgagag cgggttcgag gacatgccgc ggcgcgtgcc cgacatcacc
     1621 aggatccgcg cgctcatcgg attcgagccg aaggcgtcgc tcgacgagat cctccagctc
     1681 gtcatcgacc atcaccggac gcgctgagcc gtgccgtcca cgctgcgccg ccgcgcgcgc
     1741 cgcgccaccg agtcgctcct gctgcgacat gcgagcctcg tccggcgcct cctgctcgcc
     1801 gcgggccgcg atcgcgcccc gcgaccgccc gagatcgtgc agatcgaggc gacgaacatc
     1861 tgcaacgcga agtgcacgtt ctgcccgcgt gacgacatga cgcgccggca gggcatcatg
     1921 gactgggcgc tctaccagaa gatcgtcgac gagtgcgccg ccctcggcat cggccacgtc
     1981 cgcatgcaca actacgggga agcgttcgtc gatcggaagc tccccgagaa gatcgcctac
     2041 gccaagtcga agggcatccg cgaagtcggc gtgatcacga acggctcgct gctgggcccg
     2101 gacgccgcca gggcaatcgt gctggcgggg ctggacgcca tcaacatcag cctggacgcg
     2161 gcgggcaagg agaccttcga gtcgacgcgc gtcggcctga agtacgaccg ggtcgtcgcc
     2221 aacgtcgagg gcctcatccg catccggcgc gagctcggcc gcacgcggcc caagctcatc
     2281 ctgtccttcg ttcgccagga caactcggcc gaggagcagg ccttcatcga tcactggtcc
     2341 gccgtcgccg acaagatcca cgtcaccaac ctgcacaact gggcgggagc gctgacgcgc
     2401 cggggggagg tgaacttccc ctgttaccgc cagtggctga ccttcaccgt gctgtgggac
     2461 ggccgcgtca gcctctgctg cgccgatctt gacggccgcg tcgtgctcgg ggacctgaac
     2521 acccagacca tcgccgagat ctggaacggc gaggcctacc gccgggtgcg gcgggagcag
     2581 ctccagagcg ggggcccggc catctgccgc aactgcgatc tgccggccaa ggactcgccg
     2641 ctgtgggtgg ccaggctccg gtagccggag gcacgcgggc cggacgggct ccaactcggt
     2701 cggcgacggg cgtgggaact gggctatgat tcggtgttcc gccgggcctt cgcgggcgtc
     2761 tgccggcatg cctcggccgc gcgattcgca gtccggtcgt cttcgtttgt ccggggcccg
     2821 gccggagggg tcatgaggat acgcactcga gctgccgccg ctctgttcgc cgctggtgtg
     2881 agcgtgaccg ggaccgcctg ggcgcaagcg ctcccggccg ggccatcggt ccagcagtac
     2941 gcgggtttca cgcgctggcc agccgtggcc tacgacagcg agcacggagt gtacctcgcc
     3001 gtctggggca acggctccat ccggggccgg ttcatcaacg acgacggcac ggccgccagc
     3061 gcctggttcc agatctcgga cgcggcaccg aacgcgcaga cgccgcgcgt cgccttcagc
     3121 cccgacaccg gacagttcct ggtggtgtgg cacagctcgg acggcgtggc gggcaatccg
     3181 gaaggcacgc gcgtgcgcgg caggctcgtg gacttcagca gcgcaggccc ggtgggcccc
     3241 acgcaggtgt actcgaacgc caactacacc acgaggtggg aagtggggcc ggcggtggcc
     3301 tacgccaccg gatcgcagca gttcctggtg gcctacaaca tcttcgccgc gacgggcgcc
     3361 gagattggag cccgcctgat cgacacgacg ggcgctcccg tggggccgga gatcgccgtc
     3421 acctcatcgt ccggcgaata cgatcgcgag ccggcagtgg ggtacttccc gagcacgaac
     3481 gccttcgtgg tggcgtggtc gggaggaagc gactcgagcc cggacttcat ccgaacgagg
     3541 atgttcggcg cggcggacgg cgccgcgatc ggccccgcgg tgaccgtcgc gcagagcacg
     3601 ttcacgtacg tgccggaggt ggcctacaac ctggccaccg gccagatgct cgtcacctgg
     3661 atccagagcg acccggccgg cggaggctgg aggccgttca ggaacttcct ggacgatgcc
     3721 ggcgtcgttg tcgggacgaa cctgaggctg tcctcgaccg tcggcgcgta cgacgcgaac
     3781 tcggtggctt acaacgcgcc tgcgatgacc ttcttcctgg tcacgcacgg caacaccgag
     3841 caggacgtgg gattcgagat caccggtgac ggcatgccgg tgtcgccggc gatggttgtc
     3901 acctccgtca ccgcgccgga tctcaacggc tcctataatc cgcggatcgc ggcgagcgcg
     3961 cagacgccaa gctggctgat cgggacggcc gcgtcgttct cgagcctgtg gacgcagctc
     4021 atcacgggcg tggatctcgg cggcggcggc g
//
LOCUS       DAOSIF010000275         4049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19533_length_4049_cov_3.690285, whole genome shotgun sequence.
ACCESSION   DAOSIF010000275 DAOSIF010000000
VERSION     DAOSIF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19533_length_4049_cov_3.690285"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLT35_09430"
     CDS             complement(<1..487)
                     /locus_tag="PLT35_09430"
                     /inference="COORDINATES: protein motif:HMM:NF016555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOG29487.1"
                     /translation="MERLFSPWRMAYITGTDQPQACVFCAALSAKDKESLVLHKGRDC
                     FVVLNLYPYNNGHLMVVPNRHIAALSDATPQERCELMDLARIAELALTEIYRPQGLNV
                     GMNLGRAAGAGVVDHMHIHVVPRWLGDANFMSVVGETRVLPEDIATTAGRLRPVFQRL
                     AG"
     gene            complement(565..831)
                     /locus_tag="PLT35_09435"
                     /pseudo
     CDS             complement(565..831)
                     /locus_tag="PLT35_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831316.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(892..2685)
                     /locus_tag="PLT35_09440"
     CDS             complement(892..2685)
                     /locus_tag="PLT35_09440"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOG29488.1"
                     /translation="MANEEHGNTPDPATQAGRPTAQAHGAGPVRTIKPRAGYAEDMDP
                     TDFAALLDLYDSSFRNISEGEVVKGTVLKVTESEVVVDVGYKSEGIIPVGEFLDENGH
                     VVVQPGDTVDVLLERTEDRDGYVVLSREKAEKMKIWDEIEKAYAERKVVIGRVIERIK
                     GGLAVDIGVRAFLPGSQVDVRPVRNLDALKNQELRMRVIKVNKKRGNIVLSRKVLLEE
                     ENAEKKKYTLATLAEGKVLRGVVKNITDYGAFIDLGGIDGLLHITDMSWGRVGHPSEI
                     FKVNDEIDVVVLKYDPATERVSLGHKQLVADPWSNVSERYPIGSRVSGKVVSLTDYGA
                     FVELEAGVEGLIHVSEMTWSKRVKHPSKILNQGDAVEAMVLGVDPAARRISLGLKQIE
                     TNPWYDLASKYPIGARIVGKVRNLTEFGAFVEVEEDIDGLIHISDMSWSKRIKHPSEV
                     LKKGDKVEAVVLNIDAENQRLSLGLKQLATDIWDEFFSQRQVGDVIEGKVVRLTNFGA
                     FVELAEGIEGLIHVSEFDDSQEEKIELEVEKVYPMKIIKLSPSERKIGLSIRAMRVDQ
                     ERADWQAYTEGSGRPQATLADHFRQQQRDEH"
     gene            complement(2811..3500)
                     /gene="cmk"
                     /locus_tag="PLT35_09445"
     CDS             complement(2811..3500)
                     /gene="cmk"
                     /locus_tag="PLT35_09445"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOG29489.1"
                     /translation="MIAIDGPSGAGKGTVARAVAAALGYRHVDTGAMYRAVAWRALQL
                     GLDLDDEEAVLRVSREAAFELDGSRVSIDGDDVSAAIRTAEIDRAVTPVARQARVRAE
                     LVARQRAMAEDGPVVMEGRDIGTVVFPDAPVKIYLDALPEERARRRANDPAHSASATQ
                     ALEEIARAMARRDDHDRTRQASPLSVAPDALVIDTTRLSVAEVVERVMAAVGARGLVA
                     APLAVHERRRP"
     gene            3716..>4049
                     /locus_tag="PLT35_09450"
     CDS             3716..>4049
                     /locus_tag="PLT35_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29490.1"
                     /translation="MRARTFAAAAIVAVTALALSAPFVRSYTRAAALIVRMAGLEAAA
                     GGALAWEREPITADDTRVPTRHGEVEGADLGQLGGQAVGDLGGAVGAGVLGDRELPRT
                     PAVDAQVVV"
BASE COUNT          560 a         1432 c         1350 g          707 t
ORIGIN      
        1 ccccggcgag gcgctgaaac acgggcctga gccgtccggc tgttgtcgcg atgtcctcgg
       61 gcagcacgcg ggtctcgccg actacggaca tgaagttggc gtcgccgagc cagcgcggca
      121 ccacgtggat gtgcatgtga tcgacgacgc cggcgccggc ggcccggccg aggttcatgc
      181 cgacgttcaa tccctggggc ctgtagatct ccgtcagcgc cagctcggca atccgcgcca
      241 ggtccatcag ctcgcagcgc tcctgcggcg tcgcgtcgga gagcgccgcg atgtgccggt
      301 tgggcacgac catcagatgg ccgttgttgt agggatagag gttcaggacg acgaaacagt
      361 cccggccctt gtggaggacg agcgactcct tgtctttcgc ggagagggcg gcgcagaaca
      421 cgcacgcctg gggctggtcg gtgccggtga tgtacgccat gcgccagggt gagaagagcc
      481 gctccatcgc cgctaggcct ggggaggcgt gtcctcggcc ggccggggat ccgccgccgg
      541 cagctccgcc ggctcggtcg cgttgatgag atccttcagc tccttgccgg gcttgaagta
      601 gggcaccagc ttcgacggca cgtccacgcg ctccccggtc ttcgggttgc ggcccttgcg
      661 cggctctcgc ttgcgcaggc ggaagctgcc gaacccccgc agttcgatct tctcgccgcg
      721 ctgcagggcg aggatgatgc tgcggaagac cgtgtcgacg atgacctcgg agtgcttctt
      781 cgtgaggtcg gagacgcggg agacttcttc gacgagttca gccttggtca tggtggcccc
      841 gccgtggaga aggcgacggc ccgggcgtcc gggaccccgg gccggtcgcc gctagtgctc
      901 gtcgcgctgc tgctgccgga agtggtcggc cagggtcgcc tgcggccggc ccgatccctc
      961 ggtgtaggcc tgccagtcgg cgcgctcctg gtccacccgc atcgcgcgaa tgctcagccc
     1021 gatcttccgc tcggacgggc tgagcttgat gatcttcatc gggtagacct tctccacctc
     1081 gagctcgatc ttctcctcct gggagtcgtc gaactcggag acgtggatca ggccctcgat
     1141 gccctcggcg agctcgacga aggcgccgaa gttcgtcagc cggacgacct tgccctcgat
     1201 gacgtcgccc acctggcgct gcgagaagaa ctcgtcccag atgtccgtcg cgagctgctt
     1261 cagcccgagg gagaggcgct ggttctcggc gtcgatgttc agcacgaccg cctcgacctt
     1321 gtcgcccttc ttcagcacct cggacgggtg cttgatccgc ttgctccacg acatgtcgga
     1381 gatgtggatg aggccgtcga tgtcctcctc cacctcgacg aaggcgccga actcggtcag
     1441 gttgcgcacc ttgccgacga tgcgcgcgcc gatggggtac ttcgacgcca ggtcgtacca
     1501 gggattggtc tcgatttgct tcaggccgag cgagatgcgc cgcgccgccg gatcgacgcc
     1561 gagcaccatc gcctcgactg cgtcgccctg gttgaggatc ttcgacgggt gcttgacccg
     1621 cttgctccac gtcatctcgg acacgtggat gaggccttcg acgccggcct ccagctcgac
     1681 gaaggcgccg tagtccgtaa ggctcaccac cttgcccgac acgcggctgc cgatcggata
     1741 ccgctccgag acgttgctcc aggggtcggc caccagctgc ttgtgcccga gcgagacgcg
     1801 ctcggtcgcc gggtcgtact tgagcacgac gacgtcgatc tcgtcgttga ccttgaagat
     1861 ctccgacgga tggccgacgc ggccccacga catgtcggtg atgtgcagca ggccgtcgat
     1921 cccgccgaga tcgatgaagg cgccgtagtc ggtgatgttc ttcaccacgc cgcgcagcac
     1981 cttgccctcg gccagcgtcg ccagcgtgta cttcttcttc tcggcgttct cctcctcgag
     2041 gaggaccttg cgcgacagca cgatgttgcc gcgcttcttg ttgaccttga tgacgcgcat
     2101 ccgcagttcc tggttcttca gcgcgtccag gttgcggacc gggcgcacgt ccacctgcga
     2161 gccgggcagg aacgcccgca cgccgatgtc caccgccagc ccgcccttga tccgctcgat
     2221 cacccggccg atgacgacct tccgctccgc gtaggccttc tcgatctcgt cccagatctt
     2281 catcttctcc gctttttcgc gggacaagac gacgtagccg tcgcgatcct ccgtccgctc
     2341 cagcaggacg tccacggtgt cgcccggctg gaccaccacg tggccgttct cgtcgaggaa
     2401 ctccccgacc gggatgattc cctccgactt gtaccccacg tcgacgacca cctccgactc
     2461 ggtcaccttg agcacggtgc ccttgacgac ctcaccttcc gagatgttgc ggaagctgct
     2521 gtcgtaaaga tccagcagcg cggcgaagtc cgtcggatcc atgtcctcgg cgtagcctgc
     2581 tctgggtttg atcgtgcgca ccggcccagc cccgtgagcc tgggcggtgg ggcgtcccgc
     2641 ctgtgtcgcc gggtccgggg tgttgccgtg ctcttcgttc gccatgcgtc gcgcgtcctc
     2701 ctgcacttcg agatctgccg gtcttgggat cagcgccggc gggctgagac tggcgcggcc
     2761 atccaggatg gccacaagcg attgagtgta cgtcacttac gccgtgcagg tcaaggccga
     2821 cgccgctcgt gaaccgccag gggcgcggca acgaggcccc gcgcgccgac ggccgccatc
     2881 acccgctcga ccacctcggc aaccgacagg cgcgtcgtgt caatcaccag cgcgtccggc
     2941 gccacgctga gcggcgacgc ctggcgcgtc cggtcgtggt cgtcacgacg cgccatcgcc
     3001 cgcgcgatct cctcgagggc ctgggtcgcc gaggcggaat gcgccgggtc gttcgcccgc
     3061 cgcctcgcgc gctcctcggg cagcgcgtcc aggtagatct tcaccggcgc gtcgggaaac
     3121 acgaccgtgc cgatatcgcg cccctccatc acgaccggac cgtcttccgc catcgcgcgc
     3181 tgccgggcca ccagctcggc gcggacccgg gcctggcgcg ccacgggggt cacggccctg
     3241 tcgatctccg ccgtgcggat cgcggcggac acgtcgtccc cgtcgatgga cacgcggctg
     3301 ccgtccagct cgaaggccgc ttcccgggac acgcgcagca cggcttcttc atcgtcgaga
     3361 tcgaggccga gctggagcgc acgccaggcc accgcgcggt acatggcccc cgtgtccacg
     3421 tggcggtaac ccagcgccgc ggccacggcc cgcgcgaccg tgccctttcc cgcccccgac
     3481 gggccgtcaa tggcaatcac cgcgtgcatc attcaccggg gactggggta gggggccagg
     3541 ggccagggac gaggcgccag gcgctggtct ggccccctcg tccacgctag tcctcgaaca
     3601 gcgcgtggca agatgacagc gcgacactca cgctgacgag cccctggctc ctcagcgctt
     3661 agtccctgct ctctgccccg agtcccgagt cccggtgagt gtaacatgtc actccatgcg
     3721 ggcccggacc ttcgcggcgg cggcgattgt cgccgtcacg gcgctcgccc tgagcgcgcc
     3781 gttcgtccgc tcgtacaccc gggccgcggc gctcatcgtg cgcatggccg ggttggaggc
     3841 ggcggccggt ggcgcgctgg cctgggagcg cgagccgatc acggcagacg acacccgggt
     3901 gccgacccgc cacggggagg tggagggtgc ggacctgggg cagctcggcg gtcaagcggt
     3961 cggcgatctc ggcggtgccg tcggtgctgg cgttctcggc gatcgtgagc tgccacggac
     4021 cccggcggtg gacgctcagg tggtggtgc
//
LOCUS       DAOSIF010000276         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19562_length_4046_cov_3.276873, whole genome shotgun sequence.
ACCESSION   DAOSIF010000276 DAOSIF010000000
VERSION     DAOSIF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19562_length_4046_cov_3.276873"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..>4046)
                     /gene="rpoC"
                     /locus_tag="PLT35_09455"
     CDS             complement(311..>4046)
                     /gene="rpoC"
                     /locus_tag="PLT35_09455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG29491.1"
                     /translation="LTEEQYRKAREDYPGRFRAQMGAEAIKDLLKRIDVEQLSQELRD
                     RMKAEPSAQKKLKYAKRLKVVDAFRKSNNRPEWMILDVIPVIPPELRPLVPLDGGRFA
                     TSDLNDLYRRVINRNNRLKKLIELKAPEVIIRNEKRILQEAVDALFDNGRRGRVLRGA
                     NNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLNQCGLPKKMALELF
                     KPFIYNKLEERQMVQTIKQAKEMVEQQKSEVWDILEEVIQEHPVLLNRAPTLHRLGIQ
                     AFEPVLVEGKAIRIHPLVCTAFNADFDGDQMAVHIPLSPEAQIEASVLMMSSHNVLSP
                     ASGAPIAIPSQDIVLGIYYLTKAKRNARGEGRAFSTLDDVLLALHAEEVERLTPIRMR
                     YTGELLDLTASRDNQDVLHTELQTVAGKVINTTVGRVILNDALPKGMPYINGHLKRKG
                     LQQVVQYCYLRFGLRKTVEMLDSLKDLGFLAATTSSLSIGIDDLIVPTEKGRLVEEAR
                     QEVIHVEQQYLEGAITNGERYNKVIAIWSEVTEEIANRMFDGMQALDRDEGAFNAVYI
                     MADSGARGSKQQIRQLAGMRGLMAKPSGEIIETPITSNFREGLSVIQYFISTHGARKG
                     LADTALKTADSGYLTRRLVDVAQDVIISEPDCGTLDGIEAQAIIEGGEVIEPLRDRII
                     GRVALERIADPFTDETLAEANQEIDEEIGAAIQDAGIERVKIRSVLTCESRRGVCARC
                     YGRDLATGRLVEMGQAVGVIAAQSIGEPGTQLTMRTFHIGGTASRIADQSTLESSYDG
                     TVRFHGLQIVEGRTTGAEGAALVVMNRSGSIVIQDEKGRDRERHQLVYGAHLLVRDGQ
                     QIEAGATLAEWDPYTVSILSDGPGVVRFKDIVQGQTVHEEVDEVTGLSRLIIIDSPDE
                     KKQPAMEVRRTDGTVRRFHMPSRAHLMVTDGAEVMAGDVLAKIPRETTKTKDITGGLP
                     RVVELFEARKPRDPAIMSEIDGVAKFGGIVKGRRKVLIVPESQGETREYEIPRGVHVS
                     VQEGELVRAGEPLMDGPRNPHDILAVLGEKALQRYLVNEIQEVYRLQGVTINDKHIEV
                     ITRQMMRWVKIEDVGDTEFLIEDTVDKFRFRAENERVILEGKTPAKGRPVLLGITKAS
                     LSTESFISAASFQETTRVLTEASISGKTDYLRGLKENVTMGRLIPAGTGFEYYKSVRI
                     PADEPPPPPPPTEEELELEREMDYMVEAEGGIGLPLDIED"
BASE COUNT          521 a         1315 c         1483 g          727 t
ORIGIN      
        1 tcgcggcccg ccagagcagc gggtgcgtcc agacggcggc cagcgcggcg aacgccgcaa
       61 tcacccggcg gagttcgcgg ggcatgcccg atggtagcat gcggcgcggc agggccgggc
      121 gaggccggct ggcgcgctgc ggcctgccgg tcgccggtgt ctcgcgggcg ccgcaccggg
      181 atgccgcgcc cgccgcccgg ccgagccggc cgagccggtc gaaggaggcg agagggcggg
      241 cggcccgcct ggggccgtcc ccgggtcgtg cggcgcccgt cctagcgggc gccgcctcgg
      301 gccaacgcgc tcagtcctcg atgtcgagcg gcaggccgat cccgccttcg gcctcgacca
      361 tgtagtccat ctcccgctcg agctccagct cctcctccgt cggaggcggc ggcggcggcg
      421 gctcgtcggc cgggatgcgc accgacttgt agtattcgaa gcccgtgccg gccgggatga
      481 ggcggcccat ggtcacgttc tccttcaggc cgcgcaggta gtcggtcttg cccgagatgg
      541 acgcttcggt caggacgcgc gtggtctcct ggaacgaggc cgccgagatg aacgactccg
      601 tggagagcga cgccttcgtg atcccgagga ggaccgggcg gcccttcgcg ggggtcttgc
      661 cctccaggat cacgcgttcg ttctcggcgc ggaagcggaa cttgtccacg gtgtcctcga
      721 tgaggaactc cgtgtccccg acgtcctcga tcttcaccca ccgcatcatc tggcgggtga
      781 tgacctcgat gtgcttgtcg ttgatcgtga cgccctggag gcggtagacc tcctggatct
      841 cgttcacgag atagcgctgc agcgccttct cgccgaggac ggcgaggatg tcgtgcgggt
      901 tccggggccc gtccatgagc ggctcgccgg cgcggacgag ctcgccttcc tggacgctca
      961 cgtggacgcc gcgcgggatc tcgtactcgc gcgtctcccc ctgcgactcc ggcacgatca
     1021 gcaccttgcg gcggcccttg acgatgccgc cgaacttggc gacgccgtcg atctcgctca
     1081 tgatggccgg gtcgcggggc ttcctggcct cgaacagctc gacgacgcgc ggcaggccgc
     1141 cggtgatgtc cttggtcttg gtcgtctccc gcgggatctt cgcgaggacg tcgccggcca
     1201 tcacctccgc gccgtccgtc accatgaggt gggcgcgcga gggcatgtgg aagcggcgga
     1261 ccgtgccgtc cgtgcgccgc acctccattg ccggctgctt cttttcgtcc ggcgagtcga
     1321 tgatgatgag gcgcgagagg ccggtgacct cgtccacctc ctcgtggacc gtctggccct
     1381 ggacgatgtc cttgaagcgg acgacgcccg ggccgtcgct gaggatggag acggtgtagg
     1441 ggtcccactc cgccagggtc gcgcccgcct cgatctgctg gccgtcgcgc accaggaggt
     1501 gcgcgccgta gacgagctgg tggcgctcgc ggtcgcggcc cttctcgtcc tggatgacga
     1561 tggagccgct gcggttcatg acgaccaggg cggcgccctc ggcgcctgtc gtccggccct
     1621 cgacgatctg gaggccgtgg aagcggaccg tgccgtcgta gctcgactcg agggtggact
     1681 ggtcggcgat gcgggaggcg gtgccgccga tgtggaaggt gcgcatcgtc agctgtgtgc
     1741 cgggctcgcc gatggactgc gcggcgatga cgccgacggc ctggcccatc tcgacgaggc
     1801 ggccggtggc gaggtcgcgc ccgtagcacc tggcgcacac gccgcggcgg ctctcgcagg
     1861 tcagcaccga gcggatcttg acgcgctcga tgccggcgtc ctggatggcc gcgccgatct
     1921 cctcgtcgat ctcctggttg gcctcggcca gggtctcgtc ggtgaacggg tcggcgatgc
     1981 gctcgagggc gacgcggccg atgatgcggt cgcggagcgg ctcgatgacc tcgccgccct
     2041 cgatgatggc ctgggcctcg atgccgtcga gggtgccgca gtcgggctcg gagatgatca
     2101 cgtcctgcgc gacgtcgacg agccgccggg tcaggtagcc cgagtccgcc gtcttgaggg
     2161 cggtgtcggc cagccccttg cgcgcgccgt gcgtcgagat gaagtactgg atgacggaga
     2221 ggccctcgcg gaagttggac gtgatggggg tctcgatgat ctcgcccgac ggcttggcca
     2281 tgaggccgcg catcccggcg agctggcgga tctgctgctt gcttccccgg gcgccggagt
     2341 cggccatgat gtagaccgcg ttgaaggcgc cctcgtcgcg atcgagggcc tgcatgccgt
     2401 cgaacatgcg gttggcgatc tcctcggtga cctcggacca gatggcgatg accttgttgt
     2461 agcgctcgcc gttggtgatg gcgccctcga ggtactgctg ctcgacgtgg atgacctcct
     2521 ggcgggcctc ctcgacgagc cggcccttct cggtgggcac gatcaggtcg tcgatgccga
     2581 tggacaggct cgaggtggtc gcggcgagga agccgaggtc cttgaggctg tcgagcatct
     2641 cgacggtctt ccggaggccg aaccggaggt agcagtactg gacgacctgc tgcaggccct
     2701 tgcgcttgag gtggccgttg atgtacggca tgcccttcgg gagggcgtcg ttgaggatga
     2761 cgcggccgac ggtggtgttg atcaccttgc ccgcgacggt ctgcagctcc gtgtggagca
     2821 cgtcctggtt gtcgcgcgac gcggtcaggt ccagcagctc gccggtgtag cgcatgcgga
     2881 tgggcgtcag gcgctcgacc tcttcggcgt gcagcgcgag caggacgtcg tcgagggtgg
     2941 aaaaggcgcg gccctcgccc ctggcattgc gcttggcctt cgtgaggtag tagatgccga
     3001 ggacgatgtc ctgcgacggg atggcgatcg gggcgccgct tgcgggcgac aggacgttgt
     3061 gcgacgacat catcaggacc gaggcctcga tctgggcctc gggcgacagc gggatgtgca
     3121 ccgccatctg gtcgccgtcg aagtcggcgt tgaacgccgt gcagacgagg gggtggatcc
     3181 ggatggcctt gccctcgacc aggacgggct cgaacgcctg gatccccagc cggtgcagcg
     3241 tcggggcgcg gttgagcagc acggggtgct cctggatcac ctcctcgagg atgtcccaca
     3301 cctcgctctt ctgctgctcg accatctcct tcgcctgctt gatggtctgc accatctggc
     3361 gctcttcgag cttgttgtag atgaacggct tgaagagctc cagggccatc ttcttcggca
     3421 ggccgcactg gttcagcttg agctcgggcc cgacgacgat gacggagcgg ccggagtagt
     3481 ccacgcgctt gccgaggagg ttctggcgga accggccctg cttgcccttg agcatgtcgg
     3541 agagcgactt caggggccgg ttgttggcgc ctcgcaggac gcgcccgcgg cgtccgttgt
     3601 cgaagagcgc gtcgacggcc tcctggagga tccgcttctc gttccggatg atgacctcgg
     3661 gcgccttcag ctcgatgagc ttcttcagcc ggttgttccg gttgatgacg cggcggtaga
     3721 ggtcgttcag gtccgaggtg gcgaagcgcc cgccgtcgag cgggaccagg gggcgcagtt
     3781 cgggcgggat gaccgggatg acgtcgagga tcatccactc gggccggttg ttggacttgc
     3841 ggaaggcgtc cacgaccttg aggcgcttgg cgtacttcag cttcttctgc gcggagggct
     3901 cggccttcat ccggtcccgc agctcctgcg acagctgctc gacgtcgatc cgcttgagga
     3961 gatccttgat ggcctcggcg cccatctgcg cgcggaagcg cccggggtag tcctccctgg
     4021 ccttgcggta ctgctcctcg gtgagc
//
LOCUS       DAOSIF010000277         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19665_length_4031_cov_4.008551, whole genome shotgun sequence.
ACCESSION   DAOSIF010000277 DAOSIF010000000
VERSION     DAOSIF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19665_length_4031_cov_4.008551"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /gene="ettA"
                     /locus_tag="PLT35_09460"
     CDS             <1..1505
                     /gene="ettA"
                     /locus_tag="PLT35_09460"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOG29492.1"
                     /translation="EFLGEAYPAEGISVGFLPQEPVLDPAKSVLGNVEEGVAPTKALL
                     ARYDELNGKLGEALEPDEMAKVLDELGRLQDRIDASGAWEIDSRLELAMDALRCPPAD
                     ADVRTLSGGERRRVALCRLLLQSPDLLLLDEPTNHLDAESVAWLEHFLQAYPGTVVAV
                     THDRYFLDNVAGWILELDRGSGIPWQGNYSSWLEQKQRRLEIEEKQEARRQRTLQREL
                     EWIRMSPRARQAKGKARLNAYEAMLAEESAQRIEAAEIYIPPGPRLGDIVVEAHGLRK
                     GYGDLLLMDDVTFTLPRGGIVGVIGPNGAGKTTLFRLITGQEQPDAGTLRLGETVKVG
                     YVDQSRDALDPDKTVWQEISGGRDQVVLGKREVASRAYVSWFNFKGRDQQRKVGTLSG
                     GERNRVHLAKLLKTGCNLLLLDEPTNDLDVDTLRALEDAILAFAGCVAVISHDRWFLD
                     RIATHMLAFEGDSRVEWFEGNYQDYEADRRRRLGAEADRPHRIKYRKLTR"
     gene            1814..2833
                     /locus_tag="PLT35_09465"
     CDS             1814..2833
                     /locus_tag="PLT35_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG29493.1"
                     /translation="MRRPGVLITGASGEIGHGLIEKLAEDGATTIVTLDVRALEPSLG
                     RMVHREVTGSITDNAALDRILAEFDIDHVYHLAAYLSTRSEFTPVTAHKVNVEGTMNL
                     LEFAQKQSESHGRPVVFLYPSSIAAYGLPDLETKAKAGKVREEEYNTPSTMYGCNKLY
                     CELLGRYYARHYKQLAAEALSGKVDFRAVRFPGLISAVTVPSGGTSDYAPEMIHAAAR
                     GEAYACFVRPDTRIPFMAMPDGVEALLTLADAPRAKLTKTAYNLGAFNPSAEEVRQLV
                     LRSFRGASLTYDVDVKRQGIVDTWPADVDDSAARAEWGFAAKYDFDRAFSEYLIPTIR
                     GRYAR"
     gene            2979..>4031
                     /locus_tag="PLT35_09470"
     CDS             2979..>4031
                     /locus_tag="PLT35_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG29494.1"
                     /translation="MDLKSLIRRARGDEPADLLLKNARVVDVLGGTVEPGSVAIAGEV
                     IVGVGQCSARAEIDLGGSFVAPGFIDAHVHIESAMVPPAEFARAVVPRGTTTVVTDPH
                     EIANVLGLDGIRFMFESAKFGPLSMYVMAPSCVPATGMATSGAALHGYDLFPLKADPW
                     VLGLAEVMNFPGVVDGDDAVLEKLGAFQDRVIDGHGPGLSGAALQAYVAAGVQSDHES
                     TSADEALEKIRLGMMVMIREGSAARDLSAIVPIVTAANQHRFCFCTDDCQPSDLVERG
                     GIDHAIRSAIGLGMDPVAAIRLATWNAAQHFRLHNRGAVTPGRRADLVVFDDLHDPRP
                     RLVFRNGSVVARDGAMA"
BASE COUNT          652 a         1388 c         1404 g          587 t
ORIGIN      
        1 aggagttcct cggcgaggcg tacccggccg agggcatctc ggtcggcttt ctgccccagg
       61 agcccgtcct cgacccggcc aagagcgtgc tcgggaacgt tgaggaaggc gtggcgccga
      121 ccaaggccct cctggcccgc tacgacgagc tgaacggcaa gctcggcgag gccctcgaac
      181 ccgatgagat ggcgaaggtc ctcgacgagc tcggccgcct gcaggatcgc atcgatgcct
      241 ccggggcctg ggagatcgac tccaggctcg agctcgcgat ggacgcgctc aggtgcccgc
      301 cggcagacgc cgacgtccgc acgctgtcgg gcggcgaacg ccggcgcgtg gcgctctgcc
      361 ggctcttgct ccaatcgcct gacctgctgc tgctggacga gccgaccaac cacctcgacg
      421 cggagtcggt ggcgtggctc gagcatttcc tgcaggccta cccgggcacg gtcgtggcgg
      481 tgacgcacga ccggtacttc ctcgacaacg tcgccggctg gattctcgag ctcgatcgcg
      541 gctccgggat cccgtggcag ggcaactact cgtcgtggct cgagcagaag cagcgccgcc
      601 tcgagatcga ggagaagcag gaggcccgcc gtcagcgcac gctgcagcgc gagctggagt
      661 ggattcgcat gtcgccgcgg gcgcgccagg ccaagggcaa ggcgcggctc aacgcctacg
      721 aggccatgct cgccgaggag tcggcccagc ggatcgaggc ggcggagatc tacattccgc
      781 ccggcccccg gctcggcgac atcgtcgtcg aggcgcacgg gctgcggaag ggctacggcg
      841 acctgctgct gatggacgat gtcaccttca cgctgccacg gggcggcatt gtcggcgtca
      901 tcggccccaa cggcgcgggc aagaccacgc tgttccgcct gatcaccggg caggaacagc
      961 ccgacgccgg cacgctgcgc ctgggcgaga cggtcaaggt cggctacgtg gatcaatccc
     1021 gggacgccct ggacccggac aagaccgtct ggcaggagat ctccgggggc cgcgaccagg
     1081 tggtgctcgg caaacgcgag gtggcgtcga gggcctacgt gtcgtggttc aacttcaagg
     1141 gccgcgatca gcagcgcaag gtcgggacgc tctccggcgg cgagcgcaac cgagtccacc
     1201 tggcgaagct cctcaagacg ggctgcaacc tgctgctgct ggacgagccg accaacgacc
     1261 tcgacgtgga caccctccgc gcgctcgaag acgcgatcct ggccttcgcc ggatgcgtcg
     1321 ccgtcatcag ccacgaccgc tggtttctcg accgcatcgc gacgcacatg ctggccttcg
     1381 agggcgacag ccgcgtggag tggttcgagg gcaactacca ggattacgag gccgaccggc
     1441 gccgccggct cggcgccgag gcggaccggc cgcatcggat caagtaccgg aagctgacga
     1501 gataagagag agaagacagg agtcaggaga cagtatccgg agcccccgcc ttcgccggcc
     1561 agtccgcccg ccggattcgg cggggcaggc cgaatgccga atcgcgagcc ccgagtcccg
     1621 agcctccagc tcccggtcgc gagcccgcgt gtcgcgtcga agccggccct tcggctcgcg
     1681 ccttccactg cgtgctcagg gcgagccggc aggccttccg ggggaggcgg aagcccccga
     1741 atccggtccc cgggcgtata atccgccaac gccctgcggt ccttgctgcc gcgtcatgcg
     1801 aagaggttca actgtgcgac gacctggagt gctcatcacc ggcgccagcg gcgagatcgg
     1861 acacggactg atcgagaagc tggccgaaga cggcgcgacg acgattgtca ccctcgacgt
     1921 gagggccctc gagccgtccc tcggcaggat ggtccaccgc gaggtgaccg ggtccatcac
     1981 ggacaatgcc gctctcgacc gcatcctggc cgagttcgac atcgaccacg tctaccatct
     2041 cgcggcctac ctctcgacgc gcagcgagtt cacgcccgtc acggcccaca aggtgaacgt
     2101 cgaggggacc atgaacctcc tcgagttcgc ccagaagcag agcgagtcgc acgggcggcc
     2161 ggtcgtcttc ctctatccct cctccatcgc cgcctacggc ctgccagacc tcgagacgaa
     2221 ggcgaaggcg gggaaggtgc gcgaagagga gtacaacacc ccgtccacca tgtacgggtg
     2281 caacaagctc tactgcgagc tgctgggccg ctactacgcg cgccactaca agcagctcgc
     2341 cgccgaggcg ctctccggca aggtggactt cagggccgtg cggttcccgg gcctcatctc
     2401 ggccgtcacg gtgccgtcgg gcggcacgtc ggactacgcg ccggagatga tccacgccgc
     2461 ggccaggggc gaggcctacg cctgcttcgt gcgccccgac acccggatcc cgttcatggc
     2521 gatgcccgac ggcgtcgagg cgctgctcac gctggccgat gcgccccggg ccaagctgac
     2581 caagaccgcg tacaacctgg gggcgttcaa cccttcggcc gaggaggtcc ggcaactggt
     2641 gctgcgcagc ttccggggcg ccagcctgac gtacgacgtg gacgtcaagc gccagggcat
     2701 cgtcgacacg tggcccgccg acgtggacga ctctgccgcg cgggcggagt ggggcttcgc
     2761 ggcgaagtac gacttcgatc gggcgttctc cgagtacctg atcccgacga tccggggacg
     2821 gtacgcgagg taagggcgga attcagaatc cagcagccag aatccggagt ctgcgtgacg
     2881 gggtgacctg ctctcgctgc ctttgggatc cccattccga attccggctt cctggcttct
     2941 gattccgcat gctgaatgcg gaattccgga ttctgcgaat ggacctgaag tccctgatcc
     3001 gccgtgcgag gggcgatgag cccgccgacc tgctgctgaa gaacgcgcgg gtggtggacg
     3061 tgctgggcgg cacggtcgag cccggcagcg tcgcgattgc cggcgaggtc atcgtcggcg
     3121 tcggccagtg ctccgcccgg gcggagatcg acctcggcgg ctcgttcgtg gccccggggt
     3181 tcatcgacgc tcacgtccat atcgagagcg caatggtgcc gccggcagag ttcgcgcggg
     3241 cggtcgtgcc gcgcggcacc acgacggtgg tcacggaccc gcacgagatc gcgaacgtcc
     3301 tcggcctgga cggcatccgc ttcatgttcg agagcgccaa gttcggcccg ctgagcatgt
     3361 acgtgatggc cccgtcctgc gtgccggcga cgggcatggc cacaagcggc gcggcgcttc
     3421 acgggtacga cctgttcccg ctgaaggcgg atccctgggt gctgggcctc gccgaggtga
     3481 tgaacttccc gggggtggtg gacggcgacg acgccgtgct cgagaagctg ggggccttcc
     3541 aggaccgggt gatcgacggc cacgggccag gcctgagcgg cgccgccctt caggcctacg
     3601 tggcggcggg cgtccagtcg gaccacgagt ccacgagcgc cgacgaggcg ctcgagaaga
     3661 tccggctcgg gatgatggtc atgatccggg aaggctcggc cgcccgcgac ctgtccgcca
     3721 tcgtgcccat cgtgacggcg gccaaccagc accggttctg cttctgcacg gacgactgcc
     3781 agccctcgga cctggtggag cgcggcggca tcgaccacgc catccgcagc gcgatcggcc
     3841 tcggcatgga cccggtcgcg gccatcaggc tggcgacgtg gaacgccgcg cagcacttcc
     3901 gcctgcacaa ccgcggggcg gtcacgcccg ggcgccgcgc cgacctggtg gtgttcgacg
     3961 accttcacga cccgaggccc cgcctggtgt tcaggaacgg atcggtggtc gcgcgggacg
     4021 gcgcaatggc g
//
LOCUS       DAOSIF010000278         4021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19727_length_4021_cov_4.451336, whole genome shotgun sequence.
ACCESSION   DAOSIF010000278 DAOSIF010000000
VERSION     DAOSIF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19727_length_4021_cov_4.451336"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PLT35_09475"
     CDS             complement(<1..901)
                     /locus_tag="PLT35_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG29495.1"
                     /translation="MRLLVVSTWFPLPPDNGSRLRAYHLLRELGRRHDVTLLSFRGEG
                     RPTAGQEAALRESCRAIDTVPDGAFAERRLTARGLLSPVPRSYAQAFSADMAARIRAA
                     APGHDAVVALQVTAAFHARAAAKRLPGVFEEAELAVIRDAAGMETSAARRLRRRATWL
                     KYSRFVRSLCRRFAVTTVVSAAEREILAGIGCDPRALKVVPNGVAAADLEWPASRRCE
                     RLIYPGSLTYAANADAVRWFLASILPLIDAARPGVEFLVTGSSDGVEAAGWQGHGRMT
                     LTGHLPDVRPAVAESAVCVVPLRI"
     gene            complement(926..2236)
                     /locus_tag="PLT35_09480"
     CDS             complement(926..2236)
                     /locus_tag="PLT35_09480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG29496.1"
                     /translation="MNPRILIAVHGYPPAAGGGAELRAARTAALLARRGLEMRAFAFG
                     SHAAGPLRWDDADEDGVIVRRLNGDPAAGQDPFEASYDSQEIGRALQLALREWRPHLV
                     YLFSGYLMSSSVVRAAHEAGIPVAVNLTDYWWFCHRINLMLGGGSPCPAPSLAGCARC
                     LAESRRRWRLPAAVWPAAARAFWAAAPRARVLDRPLGIDRIRRRAEVLSQTIREVRAL
                     ISPSRHLADFYARQGIDPGRLHVIRQGVRIEQRVPRVAAAEMRFVFAGQVKAHKGIQT
                     LLDAWSMLTGPRPRRLTIYGSAAGEEAFGARIRKRVADADGVEWPGVYAPGGVWQVLA
                     EADAVVVPSLCLENSPNIVLEAQATGVPVVGSNHGGVAELVRHDHDGLLFPAGSAVGL
                     ARALQRLLDEPGLAPRLSRDGPPPRSTEAEVDETLRVLGRFLPG"
     gene            complement(2233..>4021)
                     /locus_tag="PLT35_09485"
     CDS             complement(2233..>4021)
                     /locus_tag="PLT35_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOG29497.1"
                     /translation="ETSLPEADAALGRQSIGVFNRLPQPVQHAAGVSLWLCGEFYHQR
                     ERRGRLASEGRLDGEAPDEALALAVYERDGIEGLTALEGAFAIAVWDRRTRTFVFVND
                     RFGSYPHLYAHTGGGLTLAPDLETVCCNPAVSRALDRTTVAQFVRFQQALGDRTWLQG
                     VRLLPPASQLVYRPDDDALDIRAYWDWDRIGRQPSITFDDAVDGCIGVFQRAVDAMTA
                     PPARPGIFLSGGLDGRAILGFARPDAALATITYGAAGCRDVVLAERLSRAAGRPHHWF
                     PFRDGSWVETCAPLHVAITSGLHGWYNAHGLSVLPQIRGLMDVNLSGYDGGTVLGGSL
                     DFYRDRPYREAPDPAALAASFFDAFCTKFTWPGLTDDEARALFAGESRDLFRRAPDSL
                     RDELARVTDFPPGRQADFFYHRHALARALQYQVVVQRSAIEVRCPFFDYALIDFIYSL
                     PDRIRTTPMFRRTVLTRRMPKLARIPHEKTNLLPHASPWVYYPHAFLRKLGNRINRHI
                     APVFVDRPRLYADYENYLRRELRPWAEDLLLGPRAAARGLFDPAAVRSLWERHLAGGE
                     LWTIGKVGPLISIELALRRFIDSEKRPAA"
BASE COUNT          599 a         1421 c         1481 g          520 t
ORIGIN      
        1 cgatcctcag gggcacgacg cagaccgcgc tttcggcgac ggccggacgc acgtcgggca
       61 gatgcccggt cagcgtcatg cggccatggc cctgccagcc agcggcctca acgccgtcgc
      121 tgctcccggt gaccaggaac tcgacgccgg gccgggcagc gtcgatgaga ggcaggatcg
      181 aggcgaggaa ccatcggacg gcatccgcgt ttgccgcgta ggtcagcgag cccgggtaga
      241 tgaggcgctc gcagcgtcga gaggccggcc attcgaggtc tgccgcagcc acgccgttgg
      301 gcacgacctt cagcgcgcgg gggtcgcatc cgattcccgc cagaatctcc cgctcggccg
      361 ccgagacgac ggtcgtgacg gcaaatcggc ggcagaggct gcgcacgaac ctcgagtatt
      421 tcagccaggt cgctctacgc cggagccgcc tggccgcgct cgtctccatg ccagccgcgt
      481 cccggatgac ggccagctcg gcttcttcga acacgccggg gaggcgcttc gcggcggctc
      541 gcgcgtggaa cgccgccgtc acctggaggg ccacgactgc gtcgtgcccg ggggcggcgg
      601 cgcggatgcg cgcggccatg tcggccgaga acgcctgcgc gtacgagcgg ggcacgggcg
      661 agagcagccc gcgcgccgtc aggcgccgct ctgcgaacgc cccgtcgggc acggtgtcga
      721 tcgcgcggca gctttcccgg agagcggcct cctgcccggc tgtcggcctg ccctcgccgc
      781 ggaaggacag gagcgtgacg tcgtgtcgcc ggcccagctc gcgcagcagg tggtacgcgc
      841 gaaggcggct gccgttgtcc ggcggcagcg ggaaccaggt tgaaacgacg agcagcctca
      901 tggcagcctg cacgctggcc cgcgatcagc cgggcaggaa ccggcccagg acgcgcagcg
      961 tctcatccac ctcagcctcg gtcgatcgtg gcggcgggcc atcgcgggac aatcgcggcg
     1021 ccaggcccgg ttcgtcgagc aggcgctgga gcgcgcgcgc caggccgacg gcacttccgg
     1081 cggggaacag gaggccgtcg tggtcgtggc gcacgagttc cgcgacgccc ccgtggttcg
     1141 agccaacgac cgggactccc gtcgcctgcg cctcgagcac gatgttcggg ctgttctcca
     1201 ggcacagcga cggcacgacg acggcgtccg cctccgccag gacctgccag acgccgccag
     1261 gagcgtagac cccgggccac tcgacgccgt cggcgtccgc cacgcgcttc cggatccgcg
     1321 cgccgaaggc ttcctctccc gccgccgagc cgtagatggt cagccgcctc ggtctcggac
     1381 cggtcagcat cgaccaggcg tcaagcagcg tctggattcc cttgtgcgcc ttgacctggc
     1441 cggcgaacac gaaccgcatc tcggccgcgg ccacgcgagg cacgcgctgc tcgatccgga
     1501 ctccctgtct gatcacgtgg agtcgccccg ggtcgattcc ctggcgggcg tagaagtcgg
     1561 cgaggtgccg cgacggcgag atcaaggcgc ggacctcccg aatcgtctgc gacaggacct
     1621 cggcgcggcg gcggatccgg tcgatgccga ggggccggtc gaggacgcgg gcgcgtggcg
     1681 ccgcggccca gaacgcgcgg gccgcggcgg gccagacggc agcgggcaac ctccagcggc
     1741 gccgcgattc cgccaggcag cgcgcgcagc ccgccagtga cggtgcgggg cacggggagc
     1801 cgccgcccag catcagattg atgcggtggc agaaccacca gtagtcggtc aggttcacgg
     1861 cgacggggat gcccgcctca tgcgccgctc gcaccacgct cgacgacatc aggtagccgc
     1921 tgaagaggta caccaggtgc ggccgccact cccgcagcgc gagttgcagc gcccgcccga
     1981 tctcctggct gtcgtaggag gcttcgaaag ggtcctggcc ggccgcggga tcgccgttca
     2041 gtctcctcac gatgacgccg tcctcgtcgg cgtcgtccca ccgcagcggt cccgcggcgt
     2101 gcgatccgaa ggcgaaggcg cgcatctcga gccccctgcg cgcgagcagg gctgccgtcc
     2161 gcgccgcgcg cagctccgcg ccgccgccgg cggccggcgg gtacccgtgg acggcgatca
     2221 ggatgcgcgg attcatgcgg ccggccgctt ttcgctgtcg atgaagcgcc tgagggcgag
     2281 ttcgatcgaa atgagcggcc cgaccttgcc gatcgtccag agctccccac cggccaggtg
     2341 ccgctcccag agcgaccgga ccgcggcggg atcgaagagc ccgcgcgccg cggccctggg
     2401 gccgagcagc aggtcctccg cccacggacg cagttcgcgg cggaggtagt tctcgtagtc
     2461 cgcgtacagg cggggcctgt ccacgaacac cggcgcgatg tgcctgttga tccggttccc
     2521 gagcttccgc aggaacgcgt gggggtagta gacccacggg ctcgcgtgcg gcagcaggtt
     2581 ggtcttctcg tggggaatcc gcgccagctt cggcatgcgg cgcgtcagca cggtgcgccg
     2641 gaacatcggc gtcgtgcgga tgcggtccgg cagcgagtag atgaagtcga ttagcgcgta
     2701 gtcgaagaag ggacagcgca cctcgattgc cgaccgctga acgacgacct ggtactgcag
     2761 cgcgcgcgcc agggcgtgac ggtggtagaa gaagtccgcc tggcggccgg gagggaagtc
     2821 cgtgacgcgc gccagctcgt cgcggagcga gtccggggcc cgcctgaaca ggtcccggct
     2881 ttcgccggca aacagcgcgc gcgcctcgtc gtcggtgagg ccgggccagg tgaacttcgt
     2941 gcagaacgcg tcgaagaagc tcgcggcgag cgcggccgga tcgggcgcct cccggtaggg
     3001 ccggtcgcgg tagaagtcca ggctgccgcc cagcacggtg ccgccgtcgt agccggagag
     3061 gttcacgtcc atgaggccgc ggatctgcgg gagcacgctg agcccatgcg cgttgtacca
     3121 cccgtggagg ccgctcgtga tggccacgtg cagcggcgcg cacgtttcga cccacgagcc
     3181 gtcgcggaac ggaaaccagt ggtgcgggcg cccggccgcc cgcgacagcc gctcggccag
     3241 cacgacatcc cggcatcccg ccgcgccgta ggtgatcgtc gcaagggccg cgtcgggccg
     3301 cgcgaacccg agaatggcgc ggccgtccag gccgccgctc aggaagatgc cgggccgggc
     3361 cggaggggcg gtcatcgcgt cgacggcccg ctgaaagacg ccgatgcatc cgtccacggc
     3421 gtcgtcgaag gtgatcgagg gctgccggcc aatccgatcc caatcccagt acgcccggat
     3481 gtccagcgcg tcgtcatcgg gccggtacac cagttgcgac gcgggcggaa gcaggcggac
     3541 gccctgcagc cacgtccgat cgccgagcgc ctgctgaaag cgcacgaact gcgccacggt
     3601 cgtccggtcg agcgctcggc tcaccgccgg gttgcagcag acggtctcga ggtccggagc
     3661 gagggtcagg ccgccgcccg tgtgcgcgta aagatggggg tacgacccga agcggtcgtt
     3721 gacgaacacg aacgtgcgcg ttcggcggtc ccagacggcg atggcgaacg cgccctcgag
     3781 cgcggtgagc ccctcgatgc cgtcgcgctc gtagacggcc agggcgaggg cctcatcagg
     3841 cgcctccccg tcgagccggc cctcggacgc gaggcgtccg cggcgctccc gctggtggta
     3901 gaactccccg cagagccaca ggctgacgcc ggctgcgtgc tgaacgggct gcgggagccg
     3961 gttgaagacc ccgatggact gccggccgag ggccgcgtcg gcctcgggaa gggatgtctc
     4021 g
//
LOCUS       DAOSIF010000279         4012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_19784_length_4012_cov_4.207228, whole genome shotgun sequence.
ACCESSION   DAOSIF010000279 DAOSIF010000000
VERSION     DAOSIF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19784_length_4012_cov_4.207228"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLT35_09490"
     CDS             <1..592
                     /locus_tag="PLT35_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG29498.1"
                     /translation="TLALTRTPALLLRGTEGEPVADARRTPAMDAFASGVVTRVQDAQ
                     TGPLAQAADVARHLVLPAVALALPIAAMIERLQSGAIAATLGERFVAAAVARGVPPGR
                     LLWRNALKPSLRPVAAVFGVVFGNLLSGSFIVEIVASWPGLGRLMFDALRSRDLYLVA
                     GCAAAGGAFLALGAILSDLALAAIDPRLRGREEAGA"
     gene            589..1551
                     /locus_tag="PLT35_09495"
     CDS             589..1551
                     /locus_tag="PLT35_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG29499.1"
                     /translation="MRRAGFALIGAVALLAVLAPVLTPHAAHERFADRAYAPPMAVHL
                     IDGQGAWRAPFVYPIVLADRLERRYEEDRARPVPLAWFSGGVIVRLADESRGPWLPLG
                     ADGSGRDVLARLLHGARLSLAVALLAALGALAIGTAVGGLAGSAGPVLDEVLMRLSDF
                     VLVLPAMYVVLSLRAALPLVMPPAQVFALVAGILALVGWPIVARGVRAVVAAERERDY
                     AQAARALGAGRARLLVRHLLPAARGFLATQATLLFAAFILAEATLSFVGLGFPDPSVT
                     WGVMLQEAANVAAIADAPWTLAPAAAIFLVVLGVNLAAQDAARL"
     gene            complement(1585..2316)
                     /locus_tag="PLT35_09500"
     CDS             complement(1585..2316)
                     /locus_tag="PLT35_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOG29500.1"
                     /translation="MARATFRFHGELGDLLPRQWRRREIVHVFNEPASVKDRIEAHGV
                     PHTEVALIEIGGAPAGFSRLLRNGDRVGVYPAAAGLDAPLALRPPYPRGRFVLDQHLG
                     RLAAYLRLLGLDCLHRASFPDEEVAAVAVEEGRVLLSRDRQLLMRRAVVHGGFVHATD
                     PKAQVSEVLRRFPAPERLAPFTRCLSCNGVLRPAAREDVEDRLQADTRRYYAEFRECP
                     DCRRVYWDGSHVRRMRPWVDAWLAG"
     gene            2324..3355
                     /locus_tag="PLT35_09505"
     CDS             2324..3355
                     /locus_tag="PLT35_09505"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOG29501.1"
                     /translation="MMRPTAADGERVRPGKAGDWASATTGQPTGARRTKEMETGPPFS
                     CILPLVLNLAGIYPPIATPFGRGEVDYASLQRNVERLMTTGLRGVLALGTNGEAAHVD
                     EREAERIIGAARERVPAGRVLLAGTGRQSTRAVIAATSRAAQAGADAVLVLTPFFFKS
                     RMTHDALVEHYRAIADASPVPVLLYTNTAVTGVNLAPDTVAAIAAHPNVVGMKDSNGD
                     VAQMEAVIARVPAGFALFAGSAPAIHPSILAGAAGAIVAVANVVPGLCVRLFELARAG
                     RQEEARRLQARITPLAVAVTKEHGIAGLKAAMDMAGYTGGEVRGPLRPITEQAREEIR
                     RLVDALVRD"
     gene            3526..>4012
                     /locus_tag="PLT35_09510"
     CDS             3526..>4012
                     /locus_tag="PLT35_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29502.1"
                     /translation="MRSGALLALVACLAPQASPLDQARADLQRRREALIRAYDLEADR
                     PRLDAFARTIAGAFREPAIARQAPLDRLPFVARDRRDFLERTAAFVSARLGVRLSRMT
                     VRFAGLDRGTAGRVRMVDEAAVVEVSSRHRDDDEQILAILAHEFAHIVLDAPRSGVPA
                     GS"
BASE COUNT          484 a         1497 c         1483 g          548 t
ORIGIN      
        1 cacgctggcg ctcacacgca cccccgccct gctgctgcgc ggcaccgaag gcgagcccgt
       61 ggccgatgcc cgccgcaccc ccgccatgga cgcgtttgct tcaggcgtgg tcacacgggt
      121 gcaagatgcc cagaccggcc cgctggcgca ggcggccgat gtcgcgcgcc acctggtgct
      181 gcccgccgtc gccctggccc tgccaatcgc cgccatgatt gaacgcctcc agtcgggggc
      241 gatcgccgcg accctcggcg agcggttcgt ggcggcggcc gtggcgcgcg gcgtgccgcc
      301 gggccgcctg ctctggcgca acgcgctcaa gccgtcgctg cgcccggtgg cggcggtgtt
      361 cggcgtggtc ttcggcaacc tgctgagcgg ctctttcatc gtcgagatcg tggcgtcgtg
      421 gccagggctg gggcggctga tgttcgacgc gctgcggtcg cgcgacctgt acctggtggc
      481 cggctgcgcg gcggccggcg gcgcgttcct ggccctcggc gcgatcctgt ccgacctggc
      541 gctggcggcc atcgacccgc gcctgcgcgg gcgcgaggag gccggcgcgt gaggcgcgcc
      601 ggattcgccc tcatcggggc cgtggcgctc ctcgccgtcc tcgcgcccgt gctgacgccg
      661 cacgccgcgc acgagcggtt cgccgaccgc gcgtacgcgc cgcccatggc cgtgcacctc
      721 atcgacgggc agggcgcgtg gcgcgcccct ttcgtctacc cgatcgtgct ggcggatcgg
      781 ctggaacggc gctacgagga agaccgcgcg cggcccgttc cgctggcgtg gttctcgggc
      841 ggcgtgatcg tccgcctggc cgacgaaagc cgcgggccgt ggctgccgct cggcgccgac
      901 ggctccgggc gcgacgtgct ggcgcggctc ctccacggcg cgcggctgtc gctggccgtg
      961 gcgctcctcg ccgcgctcgg cgcgctcgcc atcggcacgg ccgtcggagg cctcgccggc
     1021 tcggcagggc ccgtcctgga cgaggtcctg atgcggctat cggacttcgt gctcgtgctg
     1081 ccggcaatgt acgtggtgct gtcgctgcga gccgcgctcc ccctcgtgat gccgccagcg
     1141 caggtgttcg ccctcgtcgc cggcatcctc gcgctggtcg gctggcccat cgtcgcgcgg
     1201 ggcgtccggg ccgtcgtcgc ggccgagcgc gagcgcgact acgcgcaggc ggcacgggcg
     1261 ctcggagccg ggcgcgcgcg gctcctcgtg cgccacctcc tgccagccgc ccgcgggttc
     1321 cttgcgacgc aggccacgct gctgtttgcg gcgttcatcc tcgcggaggc caccctgtcg
     1381 tttgtcggcc tcgggtttcc cgatccgtcg gtgacgtggg gcgtgatgct gcaggaggcc
     1441 gcaaacgtcg ccgcgatcgc cgacgcgcca tggacgctcg ccccggcggc ggcgatcttc
     1501 ctcgtcgtcc tcggcgtcaa tctcgctgcg caggatgcgg cgcggctctg agcccggacg
     1561 gcgccggccg gccggcgccc gcgctcaccc ggcgagccac gcgtccaccc acgggcgcat
     1621 gcggcggacg tgcgagccgt cccagtaaac gcggcggcaa tcagggcact cgcggaactc
     1681 ggcgtagtac ctgcgcgtgt ccgcctgcag gcggtcctcc acatcctcgc gcgcggcggg
     1741 ccggagcacg ccgttgcacg acaagcagcg cgtgaagggc gccagccgtt cgggcgcggg
     1801 aaaccgccgc agcacctcgg acacctgcgc cttcggatcg gtcgcgtgga cgaagccgcc
     1861 atgcacgacc gcgcggcgca tcagcaactg ccggtcgcgc gagagcagca cgcggccctc
     1921 ctcgacggcg acggccgcca cctcctcgtc cgggaacgac gcgcggtgga ggcaatccag
     1981 ccccagcagg cgcaggtagg ccgccaatct gcccaggtgc tggtcgagca cgaatcggcc
     2041 ccggggatac ggcggccgga gggcgagcgg ggcgtcgagg ccggccgcgg cgggatacac
     2101 tcccacgcga tcgccgttgc gcaacaggcg cgagaagccg gcgggcgcgc cgccgatctc
     2161 gatgagcgcg acctcggtgt gaggcacgcc gtgggcttcg atgcggtcct tgacactggc
     2221 cggctcgttg aagacgtgaa cgatctcgcg ccggcgccac tggcggggca gcaggtcgcc
     2281 aagctcgccg tggaagcgga acgtcgcgcg ggccatcggt accatgatgc gcccaacggc
     2341 cgccgatggc gagcgcgtcc gtccgggaaa ggcaggcgac tgggcgtccg cgacgacggg
     2401 ccagccgacg ggcgccaggc ggacgaagga gatggagacc gggcctccgt tctcctgtat
     2461 ccttcccctc gtgctgaacc ttgccggcat ctatcccccc atcgccacgc cgttcgggcg
     2521 cggcgaggtg gactacgcct cgctccaacg caacgtggag cgcctgatga ccaccggcct
     2581 tcgcggcgtg ctggcgctcg gcaccaacgg cgaagccgcg cacgtggacg aacgcgaagc
     2641 cgagcgcatc atcggagcgg cacgggagcg cgtgccggcc ggccgcgtgc tcctggccgg
     2701 cacggggcgg cagtccactc gggccgtcat cgccgccacc agccgggcgg cgcaggcggg
     2761 cgccgacgcg gtcctcgtgc tgacgccctt tttcttcaag agccggatga cgcacgacgc
     2821 cctggtggag cactaccggg ccatcgccga cgcgtcgccg gtgccggtcc tcttgtacac
     2881 caacaccgcc gtgacgggcg tgaacctcgc gcccgacacg gtggcggcga ttgccgcgca
     2941 tccgaacgtc gtcgggatga aggactcgaa cggcgatgtc gcccagatgg aggctgtgat
     3001 cgcgcgggtg ccggcgggct ttgccctgtt tgccgggtcg gcgccggcga tccacccctc
     3061 gatcctcgcc ggcgcggcgg gcgccatcgt ggcagtggcc aacgtcgtgc ccggcctctg
     3121 cgtgcgcctg ttcgaactgg cgagagcggg caggcaggag gaggcgcggc ggctccaggc
     3181 acgcatcacg ccgctcgccg tggcggtgac gaaggagcac ggaatcgcgg gactgaaagc
     3241 cgcgatggac atggccggct acacgggcgg cgaggtgcgc gggcctctcc ggccaatcac
     3301 ggagcaggcg agagaggaga ttcggcggct ggtcgacgcc ctcgtcaggg attgatcccg
     3361 tcattccaga tcccggcgcc gcatggcgga ttccagggag ccgtcgccgg ggcgcgacct
     3421 ggtggcacgc gggacccgct gggccagggc ccgagcctgg tggcgccgag cgggaggcag
     3481 gccatgaccg cgccgggcgg cctgcaggcg cgataggatc tccgcatgcg gagcggcgcc
     3541 ctcctcgcgc tcgtcgcatg cctggccccc caggcctcgc cgctcgacca ggcccgcgcc
     3601 gacctgcagc ggaggcgcga agccctcatc cgggcgtacg acctggaggc tgaccgcccc
     3661 cgcctcgacg cgttcgcgcg caccatcgcc ggcgcgttcc gcgagccggc gatcgccagg
     3721 caggcgccgc tcgaccgcct ccctttcgtc gccagggacc gccgcgactt cctcgagcgg
     3781 accgcggcgt tcgtcagcgc gcgcctcggc gtgcgcctct cccgcatgac ggtccgcttc
     3841 gccggcctcg accgggggac cgccggccgc gtgcgcatgg tggacgaggc cgcggtcgtc
     3901 gaggtctcgt cgcgccaccg cgacgacgac gagcagatcc tcgcgatcct ggcccacgag
     3961 ttcgcgcaca tcgtcctgga cgcgccgcgg tccggcgtgc cggctggaag tc
//
LOCUS       DAOSIF010000280         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20094_length_3966_cov_3.221682, whole genome shotgun sequence.
ACCESSION   DAOSIF010000280 DAOSIF010000000
VERSION     DAOSIF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20094_length_3966_cov_3.221682"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLT35_09515"
     CDS             complement(<1..873)
                     /locus_tag="PLT35_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29503.1"
                     /translation="MAISKSAAAEIGPLIADLCGSDDRARDAASARLAVIGARAVPHL
                     VAACGRTRSPAALAAILKTLESAPDRRSVGLAVGLLHDAGAEPRIRGAAIALLAAHLD
                     HPQGPRALDELSAFALDAEQPDSLRLQALDAVARLLPRVTAPLRALLAVDPSAALRAW
                     AAADPAAAPAAVDPRLAVESAAAGEPADPALLRELVPAGAADAPLPTLHRLVEIARAR
                     EEAAPTPADRAEWRAVRGAVHLALARRGSRVAVYDLREALAGPAGALTAEFVDALRMA
                     GDTACLEALAERLAR"
     gene            complement(877..1737)
                     /gene="tatC"
                     /locus_tag="PLT35_09520"
     CDS             complement(877..1737)
                     /gene="tatC"
                     /locus_tag="PLT35_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOG29504.1"
                     /translation="MTPGLLAKLLPQRLRKAPPPRQLTSAKALDRPWRDDEADDDGAG
                     GRMSFLEHLDELRQRLVASAISLAAGTVVAVFFIDRIFDFIMRPLFEKLPAGSQLIYT
                     EPTEAFMLKVKIALLSGLVIGAPLIMAQLWLFIAPGLYAREKRFALPFIVSASVFFVA
                     GAAFNHYVLFPLAWQFLAGFSNDYLMFMPRIAPVFSLYAMLLVAMGLVFEMPVVILVL
                     AKMGIVTAGFLWRNIKYAVLVIFIAAAVITPTGDMVTQSLMAGPMVVLYVLSIGLAWI
                     FGKRRRAPGD"
     gene            complement(1734..2003)
                     /gene="tatA"
                     /locus_tag="PLT35_09525"
     CDS             complement(1734..2003)
                     /gene="tatA"
                     /locus_tag="PLT35_09525"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOG29505.1"
                     /translation="MFGSIGMPELVIVLVLALIIFGPRKLPELGRSLGRSIGEFRRAS
                     NELRSTLEEEIRLEEQKAPAASSASAHERASEPPSDSTSGSTSDS"
     gene            2219..3838
                     /locus_tag="PLT35_09530"
     CDS             2219..3838
                     /locus_tag="PLT35_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG29506.1"
                     /translation="MRFARLSLLVASVLVVSALAGGIFGRAAGSQDRAPERYRSFTAA
                     VRLVETGYVEKIDSERVVYGAIAGMLQTLDPHSSFLDPRTYGQMRERQQGRYYGLGIT
                     IQVVDGDITAVSLFEGSPAYKKGVRRGDVIARIDGDDTKGWTTDQAARRLRGPKGTTV
                     HVSLRRRGYDQLIELDVPRDEISIPTILPAAMIDAVTGYIRSQDFAEQTGNDLAASLK
                     ALQGRGMKRLLLDLRGNPGGPLDQAIRVSNEFLQQGQMIVYTRGRVPNADQDYRATER
                     GRYTDVPMVVLVNRNSASASEIVAGALQDHDRALVVGETTFGKALVQSVYRVSEGAAL
                     ALTTARYYTPSGRLIQRPWDGTFDEYLLYTARSQTAPSERRSADLTHTDAGRPVYGGG
                     GIEPDRRLEGPIEGFNPGRFARLLYARQAFASFAQKFSAVGDTRIAGQAHGRRFVSKD
                     FAVDEEMLGAFRDHVTSEGLAIDEAGFRQDLSFITSMIRYEIDLNLWTVEDARRRLLT
                     TDPQAQLGLASFDEAERMVLASRARASRASH"
BASE COUNT          605 a         1368 c         1488 g          505 t
ORIGIN      
        1 ccgggcgagg cgctctgcga gcgcttccag gcacgcggtg tcgccggcca tccgcagggc
       61 gtcgacgaac tccgcggtca gcgcgccggc cggcccggcc agggcctcgc ggaggtcgta
      121 gacggccacc cggctcccgc gccgggccag ggcgaggtgc accgcgccgc gcacggcacg
      181 ccactcggcc cggtccgcgg gcgtcggggc ggcctcctcg cgcgcgcggg cgatctcgac
      241 gaggcggtgg agggtgggaa gcggcgcgtc ggccgcgccg gcgggcacga gttcccgcag
      301 cagggcggga tcggcgggct cgcccgcggc cgccgattcg acggccaggc gcgggtcgac
      361 cgccgccggc gccgcggccg ggtcggccgc ggcccacgcg cggagggcgg cgctgggatc
      421 gacggcgagc agcgcgcgca gcggggccgt gacgcgcggg aggaggcggg cgacggcgtc
      481 gagcgcctgc aggcgaagcg aatccggctg ctcggcgtcg agggcgaacg cgctcaactc
      541 gtcgagggcc ctcggccctt gcgggtggtc gaggtgcgcg gcgagcagcg cgatggcggc
      601 gccgcggatc ctgggctcgg cgccggcgtc gtgcagcagt ccgacggcga gcccgacgct
      661 gcgacggtcc ggcgccgact cgagcgtctt gaggatcgcg gcaagggcgg cgggcgaccg
      721 cgtgcggccg cacgcggcca cgaggtgcgg gacggcgcgc gcgccgatca ccgccagcct
      781 cgccgacgcc gcgtcgcgcg cccggtcgtc gctcccgcac aagtcggcga tgaggggccc
      841 gatctcagcc gccgccgact tgctgatcgc cacccgtcag tccccgggcg cgcgccggcg
      901 cttgccgaag atccaggcga gcccgatgct caggacgtac aggaccacca tcggcccggc
      961 catgagcgac tgcgtgacca tgtctcccgt cggcgtgatc acggccgcgg cgatgaagat
     1021 gacgaggacg gcgtacttga tgttccgcca gaggaagccg gccgtgacga tgcccatctt
     1081 ggccagcacg aggatgacca cgggcatctc gaagacgagt cccatcgcca cgagcagcat
     1141 cgcgtacagc gagaacaccg gcgcgatgcg cggcatgaac atcaggtagt cgttcgagaa
     1201 gcccgccagg aactgccacg ccagggggaa gagcacgtag tggttgaacg ccgccccggc
     1261 cacgaagaac acgctggccg aaacgatgaa gggcaacgcg aagcgcttct cgcgggcgta
     1321 caggccgggc gcgatgaaca gccagagctg cgccatgatg agcggcgccc cgatgaccag
     1381 cccggacagg agcgcgatct tcaccttcag catgaaggcc tcggtcggct ccgtgtagat
     1441 gagctgcgat cccgcgggca gcttctcgaa gagcggccgc atgatgaagt cgaagatccg
     1501 atcgatgaag aagacggcga cgaccgtccc cgcggcgagc gagatcgccg aggcgacgag
     1561 ccgctggcgc agttcgtcga ggtgctcgag aaacgacatc cggccgcccg cgccgtcgtc
     1621 gtccgcctcg tcgtcgcgcc aggggcggtc gagcgccttc gccgacgtga gctggcgggg
     1681 cggcggcgcc ttccggagcc gttgcgggag gagcttggcg agcaggcccg gcgtcacgag
     1741 tcggacgtcg aaccggacgt ggagtcggag ggcggctccg acgcgcgttc gtgcgcggag
     1801 gcggacgagg ccgccggggc cttctgctcc tccagacgga tttcctcctc cagggtgctg
     1861 cgcagctcgt tcgacgcgcg gcggaactcc ccgatgctcc ggccgagcga ccgtccgagc
     1921 tccggcagct tccgcggtcc gaagatgatc agcgcgagca ccaggacaat gacgagctcg
     1981 ggcatgccga tggatccgaa catggaactg gctccaatgg cccctccgga cgagcggctg
     2041 cgggcgcccg ggccgggtct tcccgggacc cgtgcgggcg acggtcgtcg ccctctgccg
     2101 caccgcggac ggggcttact gcatcatagg accggagggg cgcagggtca aggacgggcg
     2161 ccgcggccgg ggcgcgaagt cgttgccgca ccagaggtta tctggtacga ttaatcgtat
     2221 gcgcttcgcc cggctgtcgc tgctggtggc ctcggtgctc gtcgtgtccg cacttgccgg
     2281 cggcatcttc ggccgcgccg ccgggagcca ggatcgggcc ccggagcgct accggtcctt
     2341 cacggctgct gtgcgcctgg tcgagacggg ctatgtcgag aagatcgaca gcgagcgcgt
     2401 cgtgtacggc gccatcgccg gcatgctcca gaccctcgat ccgcactcga gctttctcga
     2461 tccccggacc tacggacaga tgcgggagcg gcagcagggg cgctactacg gcctcggcat
     2521 caccatccag gtggtggacg gggacatcac cgcggtcagc ctcttcgagg ggtcgccggc
     2581 ctacaagaag ggcgtccgcc gcggcgacgt cattgccagg atcgacggcg acgacaccaa
     2641 gggctggacg accgaccagg ccgcgcggcg gctgcgcggg ccgaagggca ccacggtgca
     2701 cgtgtcgctc cggcgccgcg gctacgacca gctcatcgag ctggacgtgc cgcgagacga
     2761 gatcagcatc ccgacgatcc tgccggcggc catgatcgac gccgtgacgg ggtacatccg
     2821 cagccaggac ttcgccgagc agacgggcaa cgacctggcc gcgtcgctga aagcgctcca
     2881 gggccgcggc atgaagcgcc tgctgctcga tctgcgcggg aatcccggcg ggccgctcga
     2941 ccaggcgatc cgggtgtcga acgagttcct gcagcagggc cagatgatcg tctacacgcg
     3001 cgggcgggtc ccgaacgccg accaggacta ccgcgcgacg gagcgaggcc ggtacaccga
     3061 tgtgccgatg gtggtgctgg tcaaccggaa cagcgcgagc gcctccgaga tcgtggcggg
     3121 ggcgctgcag gaccacgacc gggcgctcgt cgtgggcgag acgacgttcg gcaaggcgct
     3181 ggtgcagtcg gtctaccggg tcagcgaggg cgccgccctc gccctgacga cggcccgcta
     3241 ctacacgccg agcggccggc tgatccagcg gccgtgggac gggacgttcg acgagtacct
     3301 cttgtacacg gcgcgttcgc agaccgcgcc gagcgagcgt cgatcggcgg acctgacgca
     3361 caccgacgcc ggacggccgg tctacggagg cgggggcatc gagcccgatc gccgcctcga
     3421 agggccgatc gaggggttca acccgggccg cttcgcgcgg ctgctctacg cgagacaggc
     3481 gttcgcgtcc ttcgcccaga agttctcggc cgtcggcgac acgcggatcg cggggcaggc
     3541 gcacggccgc cggttcgtct cgaaggactt cgcggtggac gaagagatgc tcggcgcgtt
     3601 ccgcgaccac gtgacgagcg aggggctggc catcgacgag gcgggcttcc ggcaggacct
     3661 gtcgttcatc acgtcgatga tccgctacga gatcgacctc aacctctgga cggtggagga
     3721 cgcgcgccgc cgcctcctca cgacggaccc gcaggcgcag ctggggctgg cctccttcga
     3781 cgaggccgag cggatggtgc tggcctccag ggccagggcg tcgcgcgcca gccactaggc
     3841 gggccgaggg gcgccgcgcg ccggggcgcg ggccggccgc cgattcacac ggttcgaagc
     3901 catgcgtttg cagcgtctgg aaatcaccgg gttcaagtcc ttcagcgacc gctccgagct
     3961 ctcctt
//
LOCUS       DAOSIF010000281         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20208_length_3947_cov_3.404933, whole genome shotgun sequence.
ACCESSION   DAOSIF010000281 DAOSIF010000000
VERSION     DAOSIF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20208_length_3947_cov_3.404933"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLT35_09535"
     CDS             complement(<1..415)
                     /locus_tag="PLT35_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29507.1"
                     /translation="MTSLLHQRITALTSAIRDEGTFELAARRVDVAAVAEAVRELWHR
                     NRAWEVLLDEGRTGITKHGGQLGWMRRWMASTGSVFNGSGRQRGKSLWCFSVLDTYCR
                     QWPGARTRWCGLTIDTARAIVGQAMADYLLTCPEAL"
     gene            complement(492..812)
                     /locus_tag="PLT35_09540"
     CDS             complement(492..812)
                     /locus_tag="PLT35_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29508.1"
                     /translation="MADGKKRGDGKPFAPGVSGNPGGLTKHQAARKSTLRAFLFSEPI
                     TEAWKKTLRQKLEDGDTTTLLWVGDQVLGKAKASLELSEDPERPLLTETAEAIIAALK
                     GGQS"
     gene            complement(840..1178)
                     /locus_tag="PLT35_09545"
     CDS             complement(840..1178)
                     /locus_tag="PLT35_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29509.1"
                     /translation="MSFTAALKKLQRAVASVSKPPRRQRKPAKRRMAPKPSADQPPRT
                     DTAVEQLVERVHAELPEVFGEPTPKTVLEMFGPWDFGTPSGRVAVAKSPPRRVIDGAA
                     WSTSGEGEAA"
     gene            complement(1175..1759)
                     /locus_tag="PLT35_09550"
     CDS             complement(1175..1759)
                     /locus_tag="PLT35_09550"
                     /inference="COORDINATES: protein motif:HMM:NF017666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HOG29510.1"
                     /translation="MALHARSAAAVAAAKTDYGTPPEVYERLDADFGPFTIDLAAHAG
                     NAKHSRYLSVIEDSLASSWDGETGFLNPPYGRGIDAWLQKARRSAIVGRAIIVQLIPA
                     NVGSSWWRKYVMGTAEDAGRLLSSAWAPESQVLWLRWEGLITGVHFWPTRIGFEGAPD
                     GAMFDSAVVVHASPNRKPPKGSTRPGSLSWGWPR"
     gene            complement(1759..2001)
                     /locus_tag="PLT35_09555"
     CDS             complement(1759..2001)
                     /locus_tag="PLT35_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29511.1"
                     /translation="MSTPTGPRLERRSSALYAARTRTMKGTLLSDGAVDELCEAFIDL
                     HSDFNAQGEALERAVAELEKRRLEDARTRRISRGRR"
     gene            complement(1998..2135)
                     /locus_tag="PLT35_09560"
     CDS             complement(1998..2135)
                     /locus_tag="PLT35_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1660 family phage protein"
                     /protein_id="HOG29512.1"
                     /translation="MSRLLCWLFGHVPAAHVDPAMSLVIHCERCHALVPGGLAYRRAR
                     R"
     gene            complement(2132..2446)
                     /locus_tag="PLT35_09565"
     CDS             complement(2132..2446)
                     /locus_tag="PLT35_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29513.1"
                     /translation="MARTKKQTELPGLEKSTHKELDTLMEEHAALASTLGETRQRMGE
                     LAEQMLAKAIELKLTVYRHETAVPPLLLTVTEGTAKLKVKPAVGAVVVDEAEVDEEGG
                     EE"
     gene            complement(2450..3001)
                     /locus_tag="PLT35_09570"
     CDS             complement(2450..3001)
                     /locus_tag="PLT35_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG29514.1"
                     /translation="MSRLFFGFDPSLSHFGYAVANVLTYGAKPIWLAAGVIVTKPLGC
                     ESKTEDNRHRFQGLARQLRELVRRHGHPDVVAVEAVALMHGRTTLTTISALGRARGLV
                     DALAAEYSVDAREFHPQALKKLIAGDRTAEKVAVEKSLVARYPELEALFAQLALANRE
                     HAADACAAVHAALTTTQHPHQEQ"
     gene            complement(2998..3165)
                     /locus_tag="PLT35_09575"
     CDS             complement(2998..3165)
                     /locus_tag="PLT35_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29515.1"
                     /translation="MTPAELRAQLEAVLRRNGVRTRLVPEQLVDRAVNEGHALELVRH
                     LEALKRDGGAV"
     gene            complement(3162..3395)
                     /locus_tag="PLT35_09580"
     CDS             complement(3162..3395)
                     /locus_tag="PLT35_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29516.1"
                     /translation="MSALPSPEIIRVEQLEDALPMWPPSSVGWAPAVESSPPSTGARR
                     PMQAADFAGWTPAGLDTLARVLRNALRANGRAR"
     gene            complement(3392..3721)
                     /locus_tag="PLT35_09585"
     CDS             complement(3392..3721)
                     /locus_tag="PLT35_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29517.1"
                     /translation="MTPSEVLRAARELVAGGWHEPMSLDAQGHICAADDEGISRFSVV
                     DALAVAAQGDVPLAMRAEDELARQLRVAGVSLHLTRWLEADGRTQAEVLRLFARAHSH
                     AVAGETR"
     gene            complement(3708..>3947)
                     /locus_tag="PLT35_09590"
     CDS             complement(3708..>3947)
                     /locus_tag="PLT35_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29518.1"
                     /translation="VVAEAAAERRRKGEDAEAERERLRLRRKAKADEAKAAVEEKQAK
                     AEEQKALFDAEKQRAVEQSAAWGARWTAEVRDDAE"
BASE COUNT          501 a         1419 c         1419 g          608 t
ORIGIN      
        1 tcagcgcctc ggggcacgtc agcaggtagt ccgccatcgc ctgcccgacg atggcccgcg
       61 cggtgtcgat ggtgaggccg caccatcgcg tgcgcgctcc gggccactgg cggcagtacg
      121 tgtcgaggac ggagaaacac cagagcgact tgccgcgctg tcgccccgag ccgttgaaga
      181 cgctgcccgt cgacgccatc caccggcgca tccacccgag ctgtccgccg tgcttcgtga
      241 tgcccgtgcg gccctcgtcg agcagcacct cccacgcgcg attgcggtgc cagagctcgc
      301 gcacggcctc agcgacagcc gcgacgtcga cacggcgcgc ggccagctcg aaggtgccct
      361 cgtcgcggat ggccgaggtg agggcggtga tgcgctggtg gaggagcgac gtcatcggac
      421 gcggtcaact cgttgcacgc caaacgcggc cagcttcgcc atccactcga gcgtcacggt
      481 gactcggtcg ctcacgactg gcctcccttc agcgcggcga tgatggcctc ggccgtctca
      541 gtgaggagcg gccgctccgg gtcctccgac agctcgagcg aggccttcgc cttgcccaac
      601 acctgatcgc cgacccacag cagcgtcgtg gtgtcgccgt cctcgagctt ctgcctcagc
      661 gtcttcttcc acgcctcggt gatgggctcg ctgaagagga aggcgcgcag ggtgctcttc
      721 cgcgccgcct ggtgtttcgt cagcccgccc gggttgccgc tcacgccggg cgcgaagggc
      781 tttccgtctc cgcgcttctt gccgtcagcc atgtgtttcc gctccttccg cgaggatgct
      841 catgccgcct caccctcgcc actggtggac cacgctgccc cgtcgatgac gcgtcgaggg
      901 ggcgacttcg cgaccgcgac acgccccgac ggcgtgccga agtcccatgg gccgaacatc
      961 tccaacaccg tcttcggcgt cggctccccg aagacctccg gcagctctgc gtgcacgcgc
     1021 tcgacgagct gctccaccgc cgtgtcggtg cgcgggggct gatcagccga cggcttcggc
     1081 gccattcgtc gtttcgcggg cttgcgctgt cgccggggcg gcttgctgac cgatgccacg
     1141 gcgcgctgca gcttcttgag ggcggcggtg aagctcatcg cggccacccc cacgacagcg
     1201 acccggggcg cgtcgagccc ttcggcggct tgcggttggg cgaggcgtgc accaccaccg
     1261 cgctgtcgaa catcgcgccg tcgggcgctc cctcgaaacc gatgcgggtg ggccagaagt
     1321 ggacgccggt gatgagcccc tcccagcgca gccagagcac ctgtgactcc ggcgcccatg
     1381 ccgacgacag cagccgcccg gcgtcctcgg ccgtccccat gacgtacttc cgccaccagc
     1441 tcgagccgac gttggcgggg atgagctgca cgatgatggc ccggccgacg atggcggagc
     1501 gccgcgcctt ctgcagccag gcgtcgatgc cgcggccgta gggcggattc aggaagcccg
     1561 tctcgccgtc ccacgacgag gccagcgagt cctcgatgac ggacaggtag cgcgagtgct
     1621 tcgcgttgcc ggcgtgcgcg gcgaggtcga tggtgaaggg gccgaagtcc gcatcgaggc
     1681 gctcgtagac ctccggcggg gtgccgtagt ccgtcttcgc ggcagccacc gcggccgcgc
     1741 tgcgtgcgtg gagcgccatc agcgacgccc cctgctgatg cgtcgcgtgc gcgcgtcttc
     1801 gaggcggcgc ttctcgagct cggcgacagc gcgctcgagc gcctcgccct gggcgttgaa
     1861 gtcgctgtgc aggtcgatga aggcctcgca cagctcgtcg acggcgccgt cgctcagcag
     1921 cgtgcccttc atcgtgcggg tgcgcgccgc gtacagcgcg gagctgcgac gctcgaggcg
     1981 cgggccggtg ggggtgctca tcggcgcgcc ctccggtacg cgagaccgcc cggcacgagg
     2041 gcgtggcagc gctcgcagtg gatgacgagc gacatggccg ggtcgacgtg ggcggccggg
     2101 acgtggccga agagccagca gagcaggcgg ctcattcctc accgccctct tcgtcgactt
     2161 ccgcctcgtc gactaccacc gcgccgacgg cgggcttcac cttcagcttc gccgtaccct
     2221 cggtgacggt gagcagcagc ggcggcaccg ccgtctcgtg ccggtacacg gtgagcttca
     2281 gctcgatcgc cttggccagc atctgctcgg cgagctcgcc catgcgctgg cgcgtctcgc
     2341 ccagcgtcga ggcgagcgcg gcgtgctcct ccatgagcgt gtcgagctcc ttgtgggtgg
     2401 acttctcgag accggggagt tccgtctgct tcttcgtgcg ggccattcgt cactgctcct
     2461 ggtgtgggtg ctgcgtggtg gtgagggcgg cgtgcaccgc ggcgcacgcg tcggcggcgt
     2521 gctcgcggtt ggcgagagcg agctgcgcga agagcgcctc gagctcgggg tagcgggcga
     2581 cgagcgactt ctcgacggcg accttctcgg ccgtgcggtc gccggcgatg agcttcttca
     2641 gggcctgcgg gtggaactcg cgggcgtcga cggagtactc ggcggcgagc gcgtcgacga
     2701 ggccccgggc gcggccgagc gcgctgatgg tggtgagcgt tgtgcggccg tgcatgaggg
     2761 ccacggcctc gacggcgacg acgtcggggt gcccatgtcg gcgcaccagc tcgcgcagct
     2821 gccgcgcgag gccctgaaag cgatgccggt tgtcctccgt cttcgactcg cacccgaggg
     2881 gcttcgtgac gatgacgccg gcggcgagcc agatgggctt cgcgccgtac gtgaggacgt
     2941 tcgccacggc gtagccgaag tgcgagagcg acgggtcgaa gccgaagaag aggcgactca
     3001 cacggcaccg ccgtcgcgct tcagcgcctc gaggtggcgc acgagctcga gcgcgtggcc
     3061 ctcgttgacg gcgcggtcga cgagttgctc gggcacgagg cgggtccgca cgccgttgcg
     3121 gcgcagcacg gcctcgagct gtgcgcggag ttccgcgggc gtcatcgcgc cctcccgttc
     3181 gcgcgcaacg cgttgcgcag gacccgcgcg agcgtgtcga gccccgcggg cgtccaaccc
     3241 gcgaagtcgg ccgcctgcat cgggcggcgc gcgccggtcg acggcggcga agactccact
     3301 gccggcgccc agccgaccga cgacggcggc cacatgggaa gcgcgtcctc gagctgctcg
     3361 acgcggatga tttcgggtga cggaagggcg ctcatcgggt ctctcctgcg actgcgtggg
     3421 aatgggcgcg ggcaaaaagg cgcagcacct cggcctgcgt gcggccgtcg gcctcgagcc
     3481 agcgggtgag gtgcaacgac acgccggcga cgcggagctg ccgcgccagc tcgtcctcgg
     3541 cgcgcatcgc gagcggcacg tcgccctgcg ccgcgacggc gagcgcgtcg acgaccgaga
     3601 agcggctgat gccctcgtcg tcggccgcgc agatgtggcc ctgggcgtcg agggacatcg
     3661 gctcgtgcca gccgccggcg acgagctcgc gcgcggcgcg caacacctca ctcggcgtca
     3721 tcgcgcacct ccgccgtcca ccgtgcgccc cacgccgcgg actgctcgac cgcgcgctgc
     3781 ttctcggcgt cgaagagcgc cttctgctcc tcggccttcg cctgcttctc ttcgacggcc
     3841 gccttcgcct cgtcggcctt cgccttgcgg cggagacgca ggcgctcgcg ctcggcctcg
     3901 gcgtcctcgc ccttgcggcg gcgctcggcc gcggcctcgg cgacgac
//
LOCUS       DAOSIF010000282         3930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20315_length_3930_cov_4.546065, whole genome shotgun sequence.
ACCESSION   DAOSIF010000282 DAOSIF010000000
VERSION     DAOSIF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20315_length_3930_cov_4.546065"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLT35_09595"
     CDS             <1..419
                     /locus_tag="PLT35_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29519.1"
                     /translation="PSCEFEAYRPGCEPGEGDATHAESNREADPRKRGISNHAGQRLT
                     FDDFPAEHWRHRRTTNLIESPFATVRLRQRVTTGAGARPTALMMAFKRLAMAEERWRK
                     VNGSEMLPHVRAGVRSVDGVQERPKTAAGEVKASAA"
     gene            472..1271
                     /locus_tag="PLT35_09600"
                     /pseudo
     CDS             472..1271
                     /locus_tag="PLT35_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611873.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1366..2100)
                     /locus_tag="PLT35_09605"
     CDS             complement(1366..2100)
                     /locus_tag="PLT35_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOG29520.1"
                     /translation="MPEKLVLDLPVAYSAVDRDQVITLRGVFACFQEAAIAHANELNA
                     GTHAMAARGEAWVLKRMAAAIERYPGYGEALRIETWSSAVRGAKGYREFLVFGGAGEP
                     VFRGSSVWVYVSLKTRSIVRVPAEVAEGFPSHPGDILCPDLEDLELPPPGPDSADVVQ
                     VSLRYSDVDAMQHVNNTSYFDLLQTALARLGRPSRPRHVRVRFGRGISSDVTAVEVRL
                     ARSPAGEPAAFSVALGNEIFARGDIG"
     gene            complement(2130..3251)
                     /locus_tag="PLT35_09610"
     CDS             complement(2130..3251)
                     /locus_tag="PLT35_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG29521.1"
                     /translation="MSASRPDHPTRREFIAAAAAAAAAPALAEGRVVVGADSAAPWQA
                     GPAGVRRFSTPGLVDLQVNGFAGVDFNDPGVTTDQVRHALDVMRRHGVTGLLPTIITS
                     TAEHFGRCARAVLRSDAKAILGIHMEGPYISPEDGPRGAHPREFTGPASLDDFKRRQD
                     EAEGRIVLVTLAPEVPGALPLIEHLRSAGVRAAIGHTGAAPELIRDAIRAGATLSTHL
                     GNGCANLIPRHPNFIWEQLAADELTASLVVDGHHLPPATVKSMIRAKTPERVILVTDA
                     ISAAGQPPGEYQIGSSRVRLDENGRVAVPGAPNLAGSALTLDRAVANTVRFAGVTIEQ
                     ALAMASTRPAAYLGVAAPGTLDVEWDAGSFTLRVLRASS"
     gene            3587..>3930
                     /locus_tag="PLT35_09615"
     CDS             3587..>3930
                     /locus_tag="PLT35_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29522.1"
                     /translation="MRPTLFPRRSIVPIAALVLAQAFFLSVASSGSPQNPSQPAQHPA
                     FKPEVGQAGKDVVWVPTPQALVDKMLDMAKVTPDDTVMDLGSGDGITVITAAKRGARA
                     VGIEYNPDMVELS"
BASE COUNT          601 a         1367 c         1400 g          562 t
ORIGIN      
        1 atccttcgtg tgagttcgaa gcataccggc ccggttgcga gccgggcgaa ggagacgcca
       61 cccatgctga gagtaaccga gaagccgacc caagaaaacg aggaatttcg aaccacgctg
      121 gacagcggct gaccttcgac gacttccccg ccgagcactg gcggcatcgg cgaaccacga
      181 acctcatcga gtcgcccttc gccaccgtgc ggctccgtca gcgcgtgacc acgggcgcgg
      241 gggcacgccc gacggcgctg atgatggcgt tcaagcggct cgcgatggcg gaagagcgct
      301 ggcgaaaggt aaatggctcg gagatgctgc cgcatgttcg cgccggcgtg cgctctgtcg
      361 acggcgtgca ggagaggcca aagaccgctg ctggagaagt gaaggcaagc gccgcctaac
      421 tcacgcggcc gacccacaac atttgacaat atctcccacg attgtcagcg ctcggtgcca
      481 cgagcgtgtc gcgtggtgag gctgtcgcgg gcggcgtact accggcctct ggtgtctcgg
      541 gtccgtgccg acgccgccgt cattgcggcc ttgaccactg tgatcgcgga tcacggccgg
      601 tgaggcttct ggaggtgctt ctattggctg cggctgcagg gccatcggtg gaatcacaaa
      661 cgggtgcatc gcgtgtatgg cgccttgcgg ctgaacttgc cccgacggac gcagcctcgc
      721 gtgtcccagc gactgcggca gccgcggacc gcgccggccg tgctgcacca cacgtgggcg
      781 ctcgacgtca tgtcggacac cctctatgag ggccggcggt ttcgcatctt gacgatcatg
      841 gacgagggca accgcgaggg cctggcggtc gaggcggaca gctcgatccc cagcgcccgc
      901 gtcggacgca tcctggagta gctcctcgcc gtccacggtc gccctgctgc cttccgggtg
      961 gacaccggcc cggagttgac ggcggagcac ttcgtcgcgt ggtgtgcgag ccaggggctg
     1021 gcgatccacg acatcccgcc gggcaagccg gaccagaacg cgtctatcga acgcttcaat
     1081 cgcagcgatc ggaccgatgt gttcgacgcc tacctctttg agtccatggc agaggcgtgc
     1141 cagctcaccg aggaatggct gagaacctac aactacgagc ggccccatga cagcctcggg
     1201 caggtgccgc ccctgacgtt ggcccgaggc cccaaaccgc cggggagtct cccttcggac
     1261 tgtctacttg acggggaagc ttacggctcc gctgcgcgat gaggtctcgc cgatactgcg
     1321 tgccggcccg ccgcgctgcg cgcgcggcgc ggcgggcaat gcggatcagc cgatgtcgcc
     1381 ccgggcgaag atctcgttcc ccagggcgac gctgaacgcc gcaggctccc cggccgggga
     1441 tcgggcgagc cggacttcaa cggccgtcac gtcgctggag atgcccctgc cgaacctgac
     1501 gcggacatgg cgcggcctgg acggccgccc gagccgggcc agcgccgtct gcagcaggtc
     1561 gaagtacgac gtgttgttca cgtgctgcat cgcgtcgacg tcggaatagc gcagcgacac
     1621 ctggaccacg tcggctgaat ccggcccggg cggcggcagt tcgagatcct cgaggtcggg
     1681 gcagaggatg tcgcccgggt gggacgggaa gccctccgcc acctcggcag gcacgcggac
     1741 gatcgaccgc gtcttgagac tgacgtacac ccagacggac gatccgcgga agacgggctc
     1801 gcccgcgccg ccgaagacga gaaactcgcg gtaccccttg gccccgcgga cggcgctcga
     1861 ccacgtctcg atgcgaagcg cctctccgta gcccgggtaa cgctcgatcg cggcggccat
     1921 gcgcttcagc acccaggctt cgccccgggc ggccatggcg tgcgtgccgg cgttgagctc
     1981 gttggcatgg gcaatggcgg cttcctggaa gcaggcgaag acgccgcgca gcgtgatcac
     2041 ctgatcgcga tcgacggcgc tgtacgccac gggcaggtcg aggacgagct tctccggcat
     2101 ggcggacggt cccccgcgag gatgacggct cagctgctgg ctcgcagcac gcggagcgtg
     2161 aaggatccgg cgtcccactc cacgtcgagc gtgccgggcg ccgcgacgcc caggtacgcc
     2221 gcagggcgcg tcgaggccat cgcgagggcc tgctcgatgg tgacgccggc aaaccgcacg
     2281 gtgttcgcga cggcgcggtc gagggtcagc gccgagccgg ccaggttcgg cgcgcccggc
     2341 acggccacgc ggccgttctc gtcgagccgc accctggacg atccgatctg atactcgccc
     2401 ggcggctggc ccgccgcgga gatggcgtcc gtcaccagga tcacgcgctc cggcgtcttc
     2461 gcgcggatca tggacttgac cgtcgccggc ggcaggtggt ggccgtcgac gacgagcgag
     2521 gccgtcagct cgtcggcggc caactgctcc cagatgaagt tcggatggcg cgggatcaga
     2581 ttggcgcagc cgttgcccag gtgcgtcgac agcgtcgctc cggcgcggat ggcgtcgcgg
     2641 atgagctcgg gtgccgcgcc ggtgtggccg atcgccgcgc gcaccccggc ggagcgcagg
     2701 tgctcgatga gcgggagcgc gccgggcacc tccggcgcga gcgtcaccag gacgatccgg
     2761 ccttccgcct catcctggcg ccgcttgaag tcgtcgagcg aggcgggccc cgtgaactcg
     2821 cgcgggtggg cgccgcgagg gccgtcctcg ggcgagatgt aggggccctc catgtggatg
     2881 cccaggatcg ccttcgcgtc cgagcgcagc accgccctgg cgcagcggcc gaaatgctcg
     2941 gcggtgctcg tgatgatcgt cggcagcagg ccggtcacgc cgtgccggcg catgacgtcc
     3001 agcgcgtggc gcacctggtc cgtcgtgacc ccgggatcgt tgaagtccac gccggcgaag
     3061 ccgttgacct gcaggtccac caggcccggc gtcgagaacc ggcgcacgcc ggccggcccg
     3121 gcctgccacg gcgccgccga gtcggcgccg accacgaccc gtccctcggc aagagcgggc
     3181 gcggcggctg ccgcggcggc ggccgcaatg aactcgcgtc gggtcgggtg gtctggtcgc
     3241 gaagcgctca tgactccccc tgcggtgggg cactcgaggg atgatagccc aggaagatgc
     3301 gcctgcgccc agcgcaggcg ccgccccctc gcgagccgcg caggccgacc ggcgccgcgg
     3361 gagtcgcgga gggcgtcctt gtccgtccgc cgtgtcccag ctggaggacg gcgcctgacg
     3421 cacgactctg gcccgccagg ccatcgcggg accgcggccc gccctcgcgg ctgcgcggcg
     3481 gcgtcgcccg aacctgggtc agacggctgc cgacgcggtc gatcgagacc atcgacacgc
     3541 gaggagcggc ggaataagat cgccagacgt ccacgtggag gctttcatgc gcccgacgct
     3601 gttcccgcgc cgatcgatcg tgccgatcgc cgctctcgtg ctcgcccagg cgttcttcct
     3661 gtccgtcgcg agttcgggct ccccgcagaa cccgtcccag ccggcccagc atccggcgtt
     3721 caagcccgaa gtggggcagg ctggcaagga cgtggtctgg gtgccgacgc cgcaggccct
     3781 cgtcgacaag atgctcgaca tggcgaaggt cacgcccgac gacaccgtca tggacctcgg
     3841 gtccggcgac ggcatcacgg tcatcaccgc ggcgaagcgc ggcgccaggg ccgtcggcat
     3901 cgaatacaac ccggacatgg tggagctgtc
//
LOCUS       DAOSIF010000283         3930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20317_length_3930_cov_2.822968, whole genome shotgun sequence.
ACCESSION   DAOSIF010000283 DAOSIF010000000
VERSION     DAOSIF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20317_length_3930_cov_2.822968"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLT35_09620"
     CDS             complement(<1..848)
                     /locus_tag="PLT35_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG29523.1"
                     /translation="MTEYTPQTIERKWQERWRAGRVFEATEDPSKPKFYCLEMFAYPS
                     GHAHVGHVRNYMIGDVMARVKRMRGFNVLHPFGWDAFGLPAENAAIKDGIHPKASTLA
                     NIAHMKGQLQRLGISYAWEREIATCLPEYYTWNQWLFLRMFERGLAYRRRSTVNWCSS
                     CRTVLANEQVVDGGCWRCQTQVTTRDLEQWFLRITHYADELLAAIDGLADWPEKVLTM
                     QRNWIGRSEGARVRFPLVDRSGSGTAGDIEVFTTRIDTIFGATFVLLAPEHDLVERFA
                     AESGDPA"
     gene            complement(928..1611)
                     /locus_tag="PLT35_09625"
     CDS             complement(928..1611)
                     /locus_tag="PLT35_09625"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG29524.1"
                     /translation="MTAPGWRDSRAKRLEVAAIAGLLYPLTAAICRTYRWRVEGREHL
                     DALAAAGQPFVLSLWHGRILPLMLYFRRRGLVALTSENFDGEWITRTITRFGYRTARG
                     SSSRNARAALRQLMREIRRGRSTAFTLDGPRGPARIAKPGAVWLAGATGAPLLPCHAE
                     ASCAWELPSWDRTQIAKPFSRVALVIGAPVFVPPGLPDDERERVRLRLEADLGVLAVR
                     AASLAHGGA"
     gene            complement(1608..3455)
                     /locus_tag="PLT35_09630"
     CDS             complement(1608..3455)
                     /locus_tag="PLT35_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG29525.1"
                     /translation="MHTAETWIFRILVFGSLAAFAAQVCVRARLVMRARDNLRVADAE
                     TGLRLRRVLAEVVFQSRTISGRPLVGLAHLAVFWGFAAFALYTLVEILAGLGLADWTG
                     SRAFRLYSLALAPFALLVAAGITALIVRRVAVRPAALGDRVSIESVVIGGFILTLMVT
                     FLLGFAVEDGAAARVNWWVHALVILGFLVLIPGSKHLHLLLSPLTVYLRSPGLGTVPN
                     LDLEAEDVGLETLGDAGRKQVIDAFTCVECGRCLDDCPARATGKALDPKALVLRTENA
                     LLARRLDAKLGDVLGTAELWQCTTCGACEAACPLGVEHLPLVIGARRGLVSNGEAPDA
                     LAAVYNHLDRRGNIWGLPVDLRQKFVQSAGLEIFDASRHEYLVWLGCAGAFEADFQKS
                     LRALFDILRAFGVTFGVLARERCTGDVAKRTGNDYQFQELARANLEAFAEAGVTRILT
                     SCPHCLRTLDVDYRGFGFAAEVVHSAAFVAGLTRGLHAAGDEPVVYHDPCYLARYAGV
                     TDAPRDLLARFGATPVEPERHGANPFCCGAGGGLLFEEREDGTRISQARLDQLVRTGA
                     PLVVTACPFCAIMLKGAQASSGARAEFADLMAFVNGRLDRATGGPLRCP"
     gene            complement(3455..>3930)
                     /locus_tag="PLT35_09635"
     CDS             complement(3455..>3930)
                     /locus_tag="PLT35_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOG29526.1"
                     /translation="RRSPPAPIRLVEAAVDPGSIRQRPEAPFRESRQAVDLSQAERIV
                     AVGRGIKEREQLALATELAAALEAEIGASRPICDAGWLPMDRQIGSSGQTVAPKLYVA
                     LGISGAIQHLVGMKGARTIVAVNKDPEAPIFEIADYGVSGDLLDIVPAVVKELKG"
BASE COUNT          613 a         1410 c         1396 g          511 t
ORIGIN      
        1 gccggatcgc ccgactcggc cgcaaagcgc tcgacgagat cgtgctcggg agccaggagg
       61 acgaaggtcg cgccgaagat cgtatcgatg cgggtggtga acacctcgat gtcgcctgcc
      121 gtccctgacc cggaccgatc caccagcgga aaccggacgc gcgcgccctc cgagcgcccg
      181 atccagttcc gctgcatcgt caggaccttc tcgggccagt cggccagccc gtcgatggcc
      241 gcgagcagtt cgtccgcgta gtgggtgatg cgcaggaacc actgctcgag atcgcgggtc
      301 gtgacctggg tctggcagcg ccagcagccg ccgtccacga cctgttcgtt cgccagcacc
      361 gtccggcagg acgagcacca gttcacggtg gaccgtctgc ggtacgcgag gccgcgctcg
      421 aacatccgga ggaacagcca ctggttccac gtgtagtact cgggcaggca ggtggcgatc
      481 tcgcgctccc aggcgtagct gatgccgagc cgctgcagct ggcccttcat gtgcgcgatg
      541 ttggcgagcg tcgaggcctt cgggtggatg ccgtccttga tggccgcgtt ctcggcgggc
      601 agcccgaagg cgtcccagcc gaacgggtgc aggacgttga acccgcgcat ccgcttcacg
      661 cgcgccatga cgtcgccgat catgtagttg cgcacgtggc cgacgtgggc gtgcccggac
      721 gggtatgcga acatctcgag gcaatagaac ttgggcttgg acgggtcctc ggtcgcctcg
      781 aagacgcggc cggccctcca gcgctcctgc cacttcctct cgatggtctg cggcgtgtac
      841 tcggtcatat ccgcacgatt atagcccacg gaggctgacg ccccggccgc cgcagggcgg
      901 gttcggctgg cagccgacgc gtgcggctca ggcgccgccg tgtgccagcg acgcggcgcg
      961 gacggcgagg acgccaaggt cggcttcgag gcgcagacgg acccgctccc gctcgtcgtc
     1021 cggcaagccg ggcggcacga agaccggcgc cccgatcacg agcgccacgc ggctgaacgg
     1081 cttggcgatc tgcgtccggt cccagctcgg caactcccac gcgcaggaag cctcggcgtg
     1141 gcacggcagc aggggcgcgc cggtggcgcc ggcgagccac accgcgcccg gcttggcgat
     1201 gcgggcaggc ccgcgaggac cgtcgagcgt gaaggccgtc gagcggcccc gccggatctc
     1261 cctcatcagc tgccgcaggg ccgcgcgcgc gttccgggaa ctggatcccc gggccgtgcg
     1321 gtacccgaac ctcgtgatgg tgcgggtgat ccactcgccg tcgaagttct cgctcgtcag
     1381 cgccacaagg ccgcgccggc ggaagtacag catcaggggc aggatgcggc cgtgccacag
     1441 cgagagcacg aacggctgcc cggcggcggc gagggcgtcc agatgctcgc gcccctcgac
     1501 ccgccagcgg taggtccggc agatcgccgc ggtgagggga tacaacaggc cggcgatggc
     1561 ggccacctcg agccgcttgg cgcgggagtc ccgccagccc ggcgcagtca cgggcaccgc
     1621 agcggcccgc ccgtggcgcg atcgaggcgc ccgttgacga aggccatgag gtcggcgaac
     1681 tcggctctgg ccccgctgct cgcctgcgcg cccttcagca tgatcgcgca gaacggacac
     1741 gccgtcacga caagcggcgc gccggtgcgg acgagctgat cgagccgggc ctggctgatg
     1801 cgcgtgccgt cttcgcgctc ctcgaacaag aggccgcctc ccgcgccgca gcagaagggg
     1861 ttggccccgt gccgctccgg ctccacgggc gtcgcgccga atcgcgcgag caggtcccgc
     1921 ggcgcgtccg tcacgccggc gtaccgcgcc aggtaacacg ggtcgtgata gacgacaggc
     1981 tcgtcccccg ccgcgtgcag gccgcgcgtc agtccggcga cgaacgccgc cgagtgcacg
     2041 acctcggcgg cgaacccgaa cccgcggtag tccacgtcca gggtccgcag gcagtgcggg
     2101 cacgaggtga ggatcctggt gacgccggcc tccgcgaagg cctcgaggtt ggcccgggcc
     2161 agctcctgga actggtagtc gttgcccgtc cgcttggcga cgtcgcccgt gcagcgctcc
     2221 ctcgcgagca cgccgaaggt gacgccgaag gcgcgcagga tgtcgaacag ggcgcgcagg
     2281 gacttctgga agtcggcctc gaaggcgccg gcgcagccga gccacacgag gtactcgtgc
     2341 cgcgacgcgt cgaagatctc caggcccgcc gactgcacga acttctgccg gaggtccacg
     2401 ggcaggcccc agatgttgcc ccgccgatcg aggtggttgt acacggcggc cagcgcatcg
     2461 ggcgcctcgc cgttcgaaac cagcccgcgc cgcgcaccga tgacgagcgg caggtgctcg
     2521 acccccagcg ggcacgccgc ctcgcaggcg ccgcacgtcg tgcactgcca cagctccgcc
     2581 gtcccgagga cgtcgcccag cttggcgtcc agcctccggg ccagcagcgc gttctcggtg
     2641 cgcagcacga gcgccttcgg atcgagcgcc ttcccggtcg cgcgggccgg gcagtcgtcc
     2701 aggcagcgtc cgcactccac gcacgtgaac gcgtcgatca cctgcttgcg cccggcgtcg
     2761 cccaaggtct cgaggcccac gtcctccgcc tcgaggtcga ggttcggcac ggtgccgagg
     2821 ccgggcgacc gcaggtacac ggtcaggggc gacagcagca gatggaggtg cttcgacccc
     2881 gggatgagca cgagaaaccc gagaatgacc agcgcgtgga cccaccagtt cacgcgggcg
     2941 gccgctccgt cctccaccgc gaaccccagc aggaaggtca ccatcagggt caggatgaag
     3001 ccgccgatca cgacggactc gatggacacg cggtcgccga gcgctgcggg ccgaacagcc
     3061 acgcgccgga cgatgagagc ggtgatcccc gcggcgacaa gcagcgcgaa gggcgccagc
     3121 gccagcgagt agaggcgaaa ggcccgcgag cccgtccagt cggccaggcc caggcccgcg
     3181 aggatttcga cgagcgtgta gagcgcgaac gcggcgaacc cccagaacac cgccaggtgc
     3241 gcgagtccca ccagcgggcg gccgctgatg gtgcgggact ggaagacaac ctcggccagc
     3301 acccgccgca gccggaggcc ggtctcggcg tctgccaccc ggaggttatc gcgcgcccgc
     3361 atcaccagcc gggcgcggac gcacacctgc gcggcgaagg cggcgaggga tccgaagacg
     3421 aggatgcgga agatccaggt ctcggcggta tgcactaccc cttgagctcc ttgacgacgg
     3481 ccgggacgat gtcgaggagg tcgcccgata cgccgtagtc ggcgatctcg aagatcgggg
     3541 cctccgggtc cttgttgacg gccacgatcg tgcgagcgcc cttcatgccg acgaggtgct
     3601 ggatcgcgcc ggaaatcccg agcgccacgt agagcttcgg cgccacggtc tggcccgaac
     3661 tgccgatctg gcggtccatc ggcagccatc cggcgtcgca aatcgggcgg gacgcgccaa
     3721 tctcggcctc gagggccgcc gccagctcgg tcgcaagggc gagctgctcc cgctctttga
     3781 tgccgcgccc gacggcgacg atgcgctccg cctggctcag gtccacggct tgccgggact
     3841 cgcggaacgg cgcctctggc cgctgcctga tcgagcctgg atccaccgcc gcctccacga
     3901 ggcggattgg cgcgggcggc gagcgtctcg
//
LOCUS       DAOSIF010000284         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20328_length_3928_cov_3.762974, whole genome shotgun sequence.
ACCESSION   DAOSIF010000284 DAOSIF010000000
VERSION     DAOSIF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20328_length_3928_cov_3.762974"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLT35_09640"
     CDS             <1..296
                     /locus_tag="PLT35_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29527.1"
                     /translation="AGGLVCLDLGTKAVASEMPQPRAVLLGIGEHRVAAHNEEHLVIE
                     VPDPDRYQPGGVVYAVPIHFCPTVARYETAHVVRNGRRAGEWTIAARNRRITI"
     gene            304..1374
                     /locus_tag="PLT35_09645"
     CDS             304..1374
                     /locus_tag="PLT35_09645"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOG29528.1"
                     /translation="MPTPLVADAHLDLSMNALEWNRDLRWSVPEIRESERGQTDRPDR
                     GRGTVSFGEMRRGNVALCAATLIARFVERGSEIPGWHSQEQAWAQTQGQLAWYRAMEE
                     AGEMAPIATVEALDRHLQRWLEGGPGPIGYVLSLEGADSLVTLHHLERQYAQGLRAVG
                     PAHYGPGVYAQGTNASGGLGERGRALLREMERLGIILDASHLCEESFWESLDVFRGPV
                     WASHNNCRVFVPHDRQLSDEQLRALIGRGAVVGVALDAWMLVPDWDRGGSTPDGRGVT
                     LSQVADNIDHICQLAGNALHAGIGSDLDGAFGTEQCPTDVDTIADLQKLPPLLASRGY
                     SEADIGNICGGNLIRFLRQAWA"
     gene            complement(1436..2632)
                     /locus_tag="PLT35_09650"
     CDS             complement(1436..2632)
                     /locus_tag="PLT35_09650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG29529.1"
                     /translation="MTTDMQRRRQVQAAATSFLALFSIVGLALYGLPLYYDFMVREFG
                     WSRTQVTSGNALSKLVVGPLFGFAAGWIIDRFGPRRMMLAGILMAGVALIGLSVMSAL
                     WMFYAFYLLNALGYVCGGPLPNQVLLSQWFREGRGKAMGFAYLGIGIGGAIVPWLSYC
                     LASGWGWRGALRALGVLIIVLAGPMAFFAKDAPQDRKGRRAEGSLAPLGAVFRRPAFY
                     LLAIGSMCSIAAVGGANQHLKLFLSLDQGYAQGDAARVISLVLAFSIAGRLLMGWLAD
                     HLPRKHVMLVIYSLVALAIPLLFFASSRGAMSLFAMLFGIGLGGEYLIIPLMAAELFG
                     VGVLGRVMGIVVTADGVAEALSPMLVGWLRDATGSYRAGFIWLVAAALAGACAIALLP
                     RRRLDR"
     gene            complement(3104..>3928)
                     /gene="argG"
                     /locus_tag="PLT35_09655"
     CDS             complement(3104..>3928)
                     /gene="argG"
                     /locus_tag="PLT35_09655"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOG29530.1"
                     /translation="QWMAAHGLPYRDSVEKAYSTDANIWGATHEAKRLEHLDTSIEIV
                     EPIMGVRFWDPAVAVASEDVTVGFVQGRPVSINGREFDDAVALVHEANTVGGRHGLGM
                     ADQIENRIIEAKSRGIYEAPGLALLFIVYERLVTGIHNEDTIDQYRANGRRLGRLLYQ
                     GRWFDPQALMLRETAQRWVARAVTGEVTLELRRGNDYSILDTTSPNLTYHPERLTMEK
                     GDRVFFTPQDRIGQLTMRNLDILDSRDKLRVYHETGLMATSPHEGLPMLPAAAVPD"
BASE COUNT          609 a         1397 c         1408 g          514 t
ORIGIN      
        1 ccgccggggg cctggtgtgc ctggacctgg gcaccaaggc ggtcgcctcc gagatgcccc
       61 agccgcgcgc ggtgctgctc gggatcgggg agcatcgcgt ggcggcgcac aacgaagagc
      121 acctcgtcat cgaggtgccc gacccggacc ggtaccagcc gggcggcgtg gtctacgccg
      181 tcccgatcca cttctgcccg accgtcgcgc gctacgagac cgcgcacgtc gtcaggaacg
      241 gccgccgcgc cggagaatgg acgattgccg cacgcaaccg ccggatcacg atctgagacg
      301 cccatgccga caccgcttgt tgccgacgcc cacctcgact tgtcgatgaa cgcgctggag
      361 tggaaccgcg acctccggtg gagcgtgccc gagatcaggg agtcggagcg cggccagacc
      421 gaccgcccgg accgcggccg cgggacggtc tccttcggcg agatgcgccg gggcaacgtc
      481 gccctctgcg cagcgacgct catcgcgcgc ttcgtcgagc gcggcagcga gatcccgggc
      541 tggcactccc aggagcaggc ctgggcccag acgcaggggc agctggcctg gtaccgcgcc
      601 atggaggagg cgggggagat ggcgccaatc gccaccgtcg aggccctcga ccgccacctc
      661 cagcgctggc tggagggcgg gcccgggccg atcgggtacg tcctgagcct cgaaggcgca
      721 gactcgctgg tcacgctgca tcacctggag cggcagtacg cgcaggggct gcgtgccgtg
      781 ggcccggccc actacggccc cggtgtctac gcccagggca ccaacgccag cggcgggctg
      841 ggcgagcgcg ggagggcgct cctgcgcgag atggagcggc tcgggatcat cctcgacgcc
      901 agccatctgt gcgaggagag cttctgggag agcctcgacg tcttccgggg gcccgtctgg
      961 gccagccaca acaactgccg cgtgttcgtg ccgcacgacc gccagctctc cgacgagcag
     1021 ctccgcgcgc tcatcggccg gggggccgtc gtcggcgttg ccctcgacgc gtggatgctg
     1081 gtgccggact gggaccgtgg aggatcgacg cccgatgggc ggggggtgac gctgtcgcag
     1141 gtggccgaca acatcgacca catctgccag ctcgccggca atgcgctcca cgcggggatc
     1201 ggctcggatc tcgacggcgc cttcggcacc gagcagtgcc cgaccgacgt ggacaccatc
     1261 gccgatctgc agaaactgcc gccgctgctc gcctcgcgcg gctactcgga ggccgacatc
     1321 gggaacatct gcggcggcaa cctgatcagg ttcctgcggc aggcctgggc ctgagcgcgt
     1381 ctccggggtc agatctacaa atatgcggat ctgaccccgc gcccggcgcg gggcttcagc
     1441 gatcgaggcg ccggcgcggg aggagcgcga tggcgcacgc gccggcgagc gcggcggcga
     1501 cgagccagat gaacccggcg cgatagctgc ccgtcgcatc gcgcagccag ccgaccagca
     1561 tgggcgagag cgcttccgcc acgccgtcgg cggtcacgac gatccccatc acgcgcccga
     1621 gcacgcccac gccgaacagc tccgccgcca tgagcgggat gatgaggtac tcgccgccca
     1681 ggccgatccc gaacagcatg gcgaacagcg acatcgcacc gcgagacgag gcgaagaaca
     1741 gcagggggat cgcgagcgcc acgagtgagt agatgacgag catcacgtgc ttcctgggca
     1801 ggtgatccgc cagccatccc atcagcaggc gtcccgcgat gctgaaggcg agcaccagcg
     1861 agatgacgcg ggcggcgtcg ccctgcgcgt acccctggtc gaggctcagg aacagcttca
     1921 ggtgctggtt cgccccgccg acggccgcga tcgagcacat gctgccgatc gcgaggaggt
     1981 agaaggccgg ccggcggaac acggcgccga gcggcgccag gctgccctcc gccctccgcc
     2041 ccttgcggtc ctggggcgcg tctttcgcga agaacgccat cggcccggcc agcacgatga
     2101 tcagcacgcc gagggcgcgg agcgccccgc gccatcccca tcccgaggcg aggcagtagg
     2161 acagccaggg cacgatggcg ccgccgatgc cgatgccgag gtaggcgaag cccatggcct
     2221 tgccgcggcc ttcgcggaac cactgcgaca gcagcacctg gttgggcagc ggcccgccgc
     2281 acacgtagcc gagggcgttg aggaggtaga acgcgtagaa catccagagc gccgacatga
     2341 cgctcagccc gatcagcgcg acgccggcca tcaggatgcc cgcgagcatc atccgcctcg
     2401 ggccgaagcg gtcgatgatc cacccggcgg cgaacccgaa gagcgggccg acgacgagct
     2461 tgctcagcgc gttgcctgac gtcacctgcg tgcgcgacca gccgaactcg cgcaccatga
     2521 agtcgtagta gagtggcagc ccgtacagcg cgaggccgac gatggagaag agcgcgagaa
     2581 aggaggtcgc ggcggcctgg acctggcggc gccgctgcat gtcggtggtc atggccaggg
     2641 cccgtgacgg acggccgcgt catccgcgcc gacgggatga gccgacagcc cggcgttcgc
     2701 gccgggctcg tcccgcctcg ccgccggaca caggcgcatt ctagcctacc cggaccatgc
     2761 cctcccgccg cgcggccccg gtgttcctcc gccgcgccgc cgtctcggcg ggactcgacg
     2821 ccaaggcagg cctcacgtcc accttcacgc cggacgcgac ggccaacccc ggcttcggcc
     2881 aggtcgaggc ggatgccccg atcacgaacc tcatcgcctg cgaggcctgc gacgagggga
     2941 agcgcccgtt ccttcctcga ggggcgcaag aggctgaggg tcgagatcga ggacgaaaac
     3001 cggctcttcc gctcgcggcg ggccgccggc ccctctcacc gggcgccgac aggccccggc
     3061 agcgggagct ccggaggtgc gcgcgccgcg gcgcacgccg gcgctagtcg gggacggctg
     3121 ccgccggcag catggggagg ccctcgtgcg gcgaggtggc catcaggccg gtctcgtggt
     3181 agacgcgcag cttgtcgcgc gaatccagga tgtcgaggtt ccgcatcgtc agctgcccga
     3241 tgcggtcctg gggcgtgaag aagacccggt cgcccttctc catggtcagc cgctcggggt
     3301 ggtaggtgag gttcgggctc gtcgtgtcga ggatcgagta gtcgttgccg cgccgcagct
     3361 cgagcgtgac ctcgcccgtc acggcgcgcg ccacccagcg ctgggcggtc tcgcgcagca
     3421 tgagcgcctg cgggtcgaac cagcgcccct ggtagagcag ccggccgagt cgccggccgt
     3481 tggcgcggta ctggtcgatg gtgtcctcgt tgtggatgcc cgtcacgagg cgctcgtaga
     3541 cgatgaacag cagggcgagg cccggggcct cgtagatgcc gcggctcttg gcctcgatga
     3601 tccggttctc gatctggtcg gccatgccga ggccgtgccg cccgccgacg gtgttggcct
     3661 cgtggacgag ggccaccgcg tcgtcgaact cgcgaccgtt gatcgacacc ggccgcccct
     3721 ggacgaaccc cacggtgacg tcctcggagg cgacggccac cgccgggtcc cagaaccgca
     3781 cccccatgat cggctcgacg atctcgatcg aggtgtccag gtgctccagg cgcttggcct
     3841 catgcgtcgc gccccagatg ttggcgtcgg tcgagtaggc cttctcgacg ctgtcgcggt
     3901 agggcagccc gtgcgcggcc atccactg
//
LOCUS       DAOSIF010000285         3926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20340_length_3926_cov_3.034100, whole genome shotgun sequence.
ACCESSION   DAOSIF010000285 DAOSIF010000000
VERSION     DAOSIF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20340_length_3926_cov_3.034100"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLT35_09660"
     CDS             complement(<1..406)
                     /locus_tag="PLT35_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29531.1"
                     /translation="MAADETTTTETVIVSQTTDGETTTVVEVVTTKTEAAAADGEDPP
                     TLIEIIEAILDPTPDTEAPLVETEGFTAIDPDVDEEVLPEEAVLADANEPADPAAIGA
                     DAGDSADEAVGPEAGDAPPTAAEASGELAAAGA"
     gene            complement(436..1512)
                     /locus_tag="PLT35_09665"
     CDS             complement(436..1512)
                     /locus_tag="PLT35_09665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG29532.1"
                     /translation="MAKLRSIGPLESAARMVSDRVGRRWVAATFNVVRLSEKQLPAVY
                     ANAVLAARILGMPVMPDIYVSGDRMWDSATYGSEADSFVILGSALLTNYHGADQLFVL
                     AREMGHCRAGHALWKTVIRFLLGEQGPRRGLGGAGVLSALNPVHLVEGALELPLLAWA
                     RQSEITADRAGMLAVGDEDVARRVLLSWSLRSVMLYRQINIEAWLEQMDDTDEQATRL
                     SEVASFSTPYITRRLKLLAGYASSPELKHYRSIIAPLLPAQPSAPTAPPAPAPPAAAG
                     RAARPPAGPPPAADDLRVSCPACKAAMRIPRAVLSGRETLNVRCANPACGKVTTLKRQ
                     PAQKPTGAVRARAAEAEGSMDAGA"
     gene            complement(1847..2353)
                     /locus_tag="PLT35_09670"
     CDS             complement(1847..2353)
                     /locus_tag="PLT35_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG29533.1"
                     /translation="MFCPACGAPFEPPAAFCGSCGTRLPQASPPSAPAAAPPAQQPEE
                     RELLRFGSFGVGITYGRPALFAWTRWNMIEVVLTDRRVCGLQSSLIARMLLSSRRGEL
                     AFQVPLADVVAMERLKVAARTAVYLKWREGAAFKEVSIEGGLGCSDHILRLHELLEQL
                     LKARTAPA"
     gene            complement(2454..2993)
                     /locus_tag="PLT35_09675"
     CDS             complement(2454..2993)
                     /locus_tag="PLT35_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOG29534.1"
                     /translation="MPFCQQCGTRNADTARFCISCGTAIAAAPSAAAAPAPPQPPPAS
                     AGPYVGPPPAGAPPLGGQAPPQSGYVQPPGAYPPPGYAGVPVAKRKSPVVAALLGFFL
                     AGFGAQAFYNGQMKKGLVQLLAVWVLWIVIVTPAAASGELGIAWIIGLAIGGAAAYDG
                     QKTAEKINAGQPVGEFSCF"
     gene            complement(3323..>3926)
                     /locus_tag="PLT35_09680"
     CDS             complement(3323..>3926)
                     /locus_tag="PLT35_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG29535.1"
                     /translation="STLLGTVPFWAILFCFMAPSFPGWATKNWLPTLFSQSLGLDMPR
                     AGPVATITIAASSFAGVVAGGLLADRWIARNLRGRIFTSAIGLGLTVPALGVMGLGAG
                     AGAAIGGAILFGVGFGMFDANNMPILCQFVSRRHRAAGYGLLNMAGVFAGAQVTRLLG
                     ASTDAGRLGRDFAFLAVPVAIALVLQLAVLRPRTADRDED"
BASE COUNT          573 a         1407 c         1435 g          511 t
ORIGIN      
        1 cggctccggc cgcggcgagt tcgccggacg cctcggccgc cgtcggaggc gcatcgcccg
       61 cctcaggacc gaccgcctcg tcggccgagt cgccagcgtc cgcgccgatc gcggcagggt
      121 cggccggttc gttcgcatcc gcgaggacgg cctcctctgg cagcacctct tcatcgacgt
      181 ccggatcgat cgcggtgaac ccctctgttt cgacgagggg ggcctccgtg tcgggcgtcg
      241 ggtcgaggat cgcttcgatg atctcgatga gcgtcggcgg gtcctccccg tcggcggccg
      301 cagcctcggt cttcgtcgtg acgacctcga caacagtcgt cgtctcgccg tccgtcgtct
      361 gggagacgat cacggtctcg gtggtggtcg tttcgtctgc ggccatggtt cagagctcct
      421 tggacagtgt cctggtcacg cgcctgcatc catgctgcct tccgcctcgg ccgcccgcgc
      481 ccggacggct cccgtgggct tctgcgcggg ctgccgcttc agcgtcgtca ccttgccgca
      541 cgcgggattc gcgcagcgca cgttgagcgt ctcgcggccg gagaggaccg cgcgcgggat
      601 gcgcatcgcc gccttgcagg cggggcacga cacgcggagg tcgtcggccg ccggtggcgg
      661 gccggccggc ggacgcgccg ccctgccggc agcggcgggc ggggccggcg ccggcggggc
      721 cgtgggcgcc gatggctggg cgggaaggag cggggcgata atcgagcggt agtgcttcag
      781 ctcgggcgac gacgcgtagc ccgcgagcag cttgaggcgg cgcgtgatgt agggcgtcga
      841 gaacgacgcg acctcggaga gccgcgtcgc ctgctcgtcg gtgtcatcca tctgttcgag
      901 ccacgcctcg atgttgatct ggcgatacag catgacggac cggagcgacc acgacagcag
      961 gacgcggcgt gccacgtcct cgtcgccgac ggccagcatg ccggcgcggt ccgcggtgat
     1021 ctccgactgg cgcgcccagg cgaggagcgg caactcgagc gcgccctcga ccaggtgcac
     1081 cggattcagc gcggagagca cgccggcgcc tcccagccct cggcgcggcc cctgctcgcc
     1141 gaggaggaag cggatgaccg tcttccacag cgcgtggccc gcgcggcagt ggcccatctc
     1201 tcgcgcgagc acgaacagct ggtccgcgcc gtgatagttg gtcaggagcg cgctgccgag
     1261 gatgacgaac gagtccgcct cgctgccgta cgtggcgctg tcccacatcc ggtcgcccga
     1321 cacgtagatg tcgggcatga ccggcatgcc gaggatgcgg gcggcgagca cggcgttcgc
     1381 gtagaccgcc ggcagctgct tctccgacag gcgcacgacg ttgaacgtgg cggcaaccca
     1441 cctccggccg acccggtcgg acaccatgcg cgccgccgat tccagggggc cgatgctccg
     1501 cagcttcgcc atcgcggccc cgtcctgggc ccactggaag gcagcggggc ttacgccgta
     1561 gcccggggcg agggggcgaa gccggctgca ggccgggcac acgtgcgtgc cggcgggcag
     1621 cgtcgacggg tcattcccgc acgccggaca cgcggcggga acgggcggcg ttcctgcgta
     1681 aggagccgcg cctggcgcaa ccgggggccc aggcgccgac gctggcgcgg caggcatcag
     1741 tcgaaccccg cagatcgtgc agaactggct ccccggggga aacggcccgt ggccgctggc
     1801 gcagtacacg gcggcatggc cctggtctgc cattggagcg cctccctcaa gccggcgcgg
     1861 ttcgcgcctt gagcagctgt tccagcaatt cgtggagccg caggatgtgg tcgctgcacc
     1921 caaggccgcc ttcgatcgaa acctccttga aggcggcgcc ctccctccac ttcaggtaga
     1981 ccgctgtgcg cgcggcgacc ttcagccgct ccatcgcgac gacgtccgcg agcggaacct
     2041 ggaaggccag ctccccgcgt ctggacgaca ggagcatgcg cgcgatgaga gaactctgca
     2101 ggccgcacac ccgccgatcg gtgagcacga cctcgatcat gttccacctg gtccacgcga
     2161 agagggccgg ccggccgtac gtgatgccga ctccgaagga gccgaaccgc aggagctccc
     2221 gctcctccgg ctgctgcgcc ggcggcgccg ctgccggcgc ggatggcggt gacgcctgcg
     2281 gcaggcgcgt tccgcacgag ccgcagaagg ccgctggcgg ttcgaacggc gcgccgcatg
     2341 cgggacagaa catactgata ccctccggag cgaaccgccg gctgcaagcc cgcgggcgaa
     2401 actacgtcac tgcctcgggg aggcgatgag ggggcgtcgg ttgcccggag ccgctagaag
     2461 caggagaact cgcccaccgg ctggccggcg ttgatcttct ccgcggtctt ctgtccgtcg
     2521 tacgcggcgg cgccgccgat ggccagcccg atgatccacg cgatgccgag ttcgcccgag
     2581 gccgccgccg gcgtgacgat gacgatccac agcacccaga ccgcgagcag ctgcacgagg
     2641 cccttcttca tctggccgtt gtagaacgcc tgcgccccga accccgccag gaagaacccg
     2701 agaagcgccg ccacgacggg cgacttccgc ttggcgacgg gaacgccggc atagccggga
     2761 ggcgggtagg ctcccggggg ctggacgtac ccgctctgcg gcggggcctg tccgcccagg
     2821 ggcggcgccc ctgcgggcgg cggaccgacg tacggcccgg ccgacgctgg aggcggctgc
     2881 ggcggagccg gcgcggcggc agccgacggc gcggccgcga tcgcggtccc gcatgaaata
     2941 cagaaacgag cggtgtcggc gttcctggtt ccacactgct ggcagaaagg catgcgcccc
     3001 tccctcggtt gggaacatgg ccgccctcgg gccgttggcg ctctcacctg ccgtggccgc
     3061 gggcaatgcg ccagacgata tgtccgcccc ccggccttgt caagaacccg cgagcggcac
     3121 cggccggccc cggaagacgg gacggcagac gcggcaggcc cgaggaagcg gcagccgcgc
     3181 tggcgcgggc cgccttcacg tcgagccgcg gccgacacgc aggacgccac ggccggcggc
     3241 gatacgcctg acgcgccccg gccgggccgc gggccccgag tcgtggcggg aggccgccca
     3301 acggtcctcg acagcgccta ggctagtctt cgtcccgatc ggccgttcgg gggcgaagca
     3361 cggccaactg cagcaccagc gcgatggcaa cgggcacggc caggaaggcg aagtcgcgcc
     3421 ccaggcggcc ggcgtcggtc gaggcgccga gcagcctggt cacctgcgcg ccggcgaaca
     3481 cgccggccat gttcagcagg ccgtagccgg ccgcgcggtg gcgccgggag acgaactggc
     3541 agaggatcgg catgttgttc gcgtcgaaca tgccgaaccc gactccgaac aggatcgcgc
     3601 cgccgatagc ggcgccggcg ccggcgccca gccccatgac gccgagcgcc ggcacggtca
     3661 gccccagccc gatcgcgctc gtgaagatgc gtccgcgcag gttgcgtgcg atccagcggt
     3721 cggcgagcag gccgcccgcg accactcccg cgaagctgga cgccgcgatc gtgatcgtcg
     3781 ctaccgggcc ggcgcgcggc atgtcgaggc cgaggctttg tgagaagagc gtcggcagcc
     3841 agttcttggt ggcccagccg gggaagctcg gcgccatgaa acagaagagg atcgcccaga
     3901 acggcacggt cccaagcagg gtcgac
//
LOCUS       DAOSIF010000286         3923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20362_length_3923_cov_3.648139, whole genome shotgun sequence.
ACCESSION   DAOSIF010000286 DAOSIF010000000
VERSION     DAOSIF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20362_length_3923_cov_3.648139"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLT35_09685"
     CDS             <1..607
                     /locus_tag="PLT35_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG29536.1"
                     /translation="DQPGSDARPAPREEPAERAGSGTEAGTPAPGPAAEREPPADDPV
                     LRGLRGPRPPVKSGSDGSGSGGGVLGDALRNVTRYVPQQMFDNPQGGGQFGSAIQFDT
                     KGVEFGPWVRRFIAQIKRNWFIPYAAMSLSGHVAVSFNVHKDGTITQLGVVEPSGVEA
                     FNNSSFNAIAASNPTQPLPPEYPADRAFFTVTFYYNEIPPR"
     gene            699..1691
                     /gene="miaA"
                     /locus_tag="PLT35_09690"
     CDS             699..1691
                     /gene="miaA"
                     /locus_tag="PLT35_09690"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG29537.1"
                     /translation="MTARAQPRVVAAVLGPTASGKSALGVALAERIGGEIVGCDSTAV
                     YRGFDIGTDKVPLCEQRGIPHHCVDIVEPDRDYTAADYARDAMAAIQAVHARGRLPVI
                     VGGTGFYYRALVRGLFPGPGRDDRLRGRLEALAARRGPERLHRLLARIDPPSAARIRP
                     RDEMRVVRALEVYYQSGRTLTEHFADTRAPLAGWTVVAIALRPPWEEIARRVAERVDR
                     QFARGIEDEVRAILARGVPPSARPFTGYVYRTVLELVRGVRDAASAREVIVRENCRYA
                     RRQLIWFRKEPNLVWIHRAGDRAEALGEAEPLVAAQMVARQAPGPPGEHPTNVP"
     gene            1681..1923
                     /gene="hfq"
                     /locus_tag="PLT35_09695"
     CDS             1681..1923
                     /gene="hfq"
                     /locus_tag="PLT35_09695"
                     /inference="COORDINATES: protein motif:HMM:NF028518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOG29538.1"
                     /translation="MSHSPDARTPSEPNVQDAFLNVARREHQPVAIRMMDGTEVEARV
                     RSFDRFAVLLDQDGADHLIFKHAIASIRVARPAAQR"
     gene            1971..3161
                     /locus_tag="PLT35_09700"
     CDS             1971..3161
                     /locus_tag="PLT35_09700"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOG29539.1"
                     /translation="MAQSPFRRAFVVVLDSLGVGELPDAGAYGDEGSNTLGNVAARVP
                     LRIPALNALGLGRVVALAGVPAEPSPRGAFGRMAEQSPGKDSVTGHWELMGLQLDRAF
                     PTFPNGFPEASIREFERRIGRSILGNVVASGTQIIEDLGAEHLRTGRPIVYTSADSVF
                     QIAAHEDVIPVPELYRLCEIAYGIYVEGMGLGRVIARPFVGVPGRFERTANRHDFAVP
                     PPAETLLDRLTARGIPVHAIGKITDLFAGQGIATSSRTRSDSDVMDEVVRAVADAPPG
                     LVFANLVDFDAQFGHRNDVEGYAANLERFDRRLAQMLPLLRRDDLLALTADHGNDPTT
                     PSTDHSREYVPILFYGDQVAGGVDLGTRSTFADLGQTLAGVFGVGPLGCGTSMLGDIR
                     PAAA"
     gene            3178..>3923
                     /locus_tag="PLT35_09705"
     CDS             3178..>3923
                     /locus_tag="PLT35_09705"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOG29540.1"
                     /translation="MSLIREALERREAETLAPQAAKSAHTRGRLNPEPEDDVRPAFQR
                     DRDRIIHSKAFRRLKHKTQVFFAPEGDHYRTRLTHSLEVSQIARTIAKALSLNEELTE
                     AIALGHDLGHTPFGHQGERVLAELVPGGFEHDVQSLRIVDVLERDGAGLNLTWETRDG
                     ISRHSKGKRGAPVGPDVLVKAATLEGQIARAADLIAYVNHDIDDAIRARIIGAADLPP
                     APIGVLGATHSARIGTLVKDVVVETRQAGL"
BASE COUNT          568 a         1407 c         1409 g          539 t
ORIGIN      
        1 agaccagccg gggagcgatg cgcgccctgc cccgagagaa gaacccgccg agcgggctgg
       61 ctccgggacc gaggccggga cgccggcgcc cggcccggcg gcggagcggg aaccgccggc
      121 cgacgatccc gttcttcggg gcctgcgcgg gccgcggccg cccgtcaagt ccggcagcga
      181 cgggtcggga agcggcgggg gagtccttgg cgacgcgctg cgcaacgtga cgcggtacgt
      241 gccgcagcag atgttcgaca acccgcaggg gggcggccag ttcggctcgg ccatccagtt
      301 tgacacgaag ggcgtcgagt tcgggccgtg ggtgcggcgg ttcatcgctc agatcaagcg
      361 caactggttc atcccgtacg cggcgatgtc gctgagcggg cacgtcgccg tgtcgttcaa
      421 cgtgcacaag gacggcacga tcacgcaact cggcgtcgtc gagcccagcg gggtcgaggc
      481 gttcaacaac tcgtcgttca acgccatcgc ggcgtcgaac cccacgcagc ccctcccgcc
      541 ggagtacccg gcggaccggg ccttcttcac ggtgacgttc tactacaacg aaatccctcc
      601 gcgctgacga ccatgcccac gcgcgcgcaa cggctcggct ggctgatcct cctcggcgcc
      661 ctcgtcacct ggacgctcgt gaagctggcg ggcgcgccat gacggcgcgc gcgcagccac
      721 gggtcgtcgc cgccgtgctg ggcccgacgg cgagcggcaa gagcgcgctc ggggtggcgc
      781 tggccgagcg gatcggcggc gagatcgtcg ggtgcgattc gacggccgtc tatcgcgggt
      841 tcgacatcgg caccgacaag gtcccgctct gcgagcagcg gggcattccg caccactgcg
      901 tggacattgt ggagccggac cgcgactaca ccgccgccga ctacgcgcgt gacgcgatgg
      961 cggcgatcca ggccgtccac gcgcgcgggc ggctgccggt gatcgtcggc ggcaccggtt
     1021 tctactaccg cgcgctcgtc agggggcttt ttcccggccc gggccgcgat gaccgcctgc
     1081 gcgggcggct ggaggcgctg gcggcgcgcc gcggtcccga gcggctgcac cggctgcttg
     1141 cacgcatcga tccgccctcg gcggcgcgca tccggccgcg agacgagatg cgggtggtgc
     1201 gggcgctcga ggtgtactac cagagcggcc ggacgctgac cgagcacttc gccgacaccc
     1261 gggcgccgct ggcggggtgg acggtcgtcg ccatcgcgct gcggcccccg tgggaggaga
     1321 tcgcccggcg cgtcgccgag cgggtcgacc gccagttcgc gcgcggcatc gaagacgagg
     1381 tgcgggccat cctggcgcgg ggcgtgccgc cgtcggcccg gccgttcacg ggctacgtgt
     1441 accgcacggt gctcgaactc gtcaggggcg tccgcgacgc cgcgtcggcg cgcgaggtga
     1501 tcgttcgcga gaactgccgc tacgcgcgga ggcagttgat ctggttccgc aaagagccta
     1561 atctagtgtg gattcatcga gcgggcgatc gggcggaggc gcttggcgag gccgagccgc
     1621 tcgtggccgc ccagatggtc gcgcggcagg ccccgggccc gccaggggag catcccacga
     1681 atgtcccata gtcccgacgc gagaacgccg agcgagccga acgttcagga tgcgtttctc
     1741 aacgtcgccc gtcgcgagca ccagcccgtc gccatccgca tgatggacgg caccgaggtc
     1801 gaggcgcggg tcaggagctt cgatcggttc gcggtgctcc tcgatcagga cggcgccgat
     1861 cacctcatct tcaagcacgc gatcgcctcg atccgcgtgg cgcggccggc ggcccagcgg
     1921 tagcggcggg cccgcccgcc ggggcgagcg tcgaatcgtc cggatcgcac atggcacagt
     1981 ccccgttccg gcgcgccttc gtcgtcgtcc tcgacagcct gggagtcggc gagcttcccg
     2041 acgccggcgc ttacggggac gaggggagca acacgctcgg caacgtggcg gcgcgagtgc
     2101 cgctccgcat cccggccctc aacgccctcg ggctcgggcg cgtcgtggcc ctggccggcg
     2161 tgccggccga gccgtcgccg cgcggcgcct tcggccgcat ggccgagcag tcgcccggca
     2221 aggactcggt gaccggccac tgggagctga tggggctgca gctggaccgc gcgttcccga
     2281 cgttcccgaa cggcttcccc gaagcgtcca tccgggagtt cgagcggcgg atcgggcgct
     2341 ccattctcgg caacgtcgtg gcgtccggca cccagatcat cgaggacctt ggcgccgagc
     2401 acctgcgcac cggcaggccg atcgtctaca cgtcggccga cagcgtgttt cagatcgccg
     2461 cgcacgaaga cgtcattccc gtgccggagc tctaccgact ctgcgagatc gcatacggga
     2521 tctacgtcga gggcatgggg ctgggccgcg tcatcgcgcg gccgttcgtc ggggtgccgg
     2581 gccgcttcga gcgcaccgcc aaccgtcacg acttcgcggt gccgcctcca gccgagaccc
     2641 tcctcgaccg cctgacggcg cggggcattc ccgtccacgc catcggcaag atcaccgatc
     2701 tcttcgccgg ccagggcatc gccacgtcga gccggacccg gtccgactcg gacgtgatgg
     2761 acgaggtcgt ccgcgccgtc gccgatgcgc cgccggggct ggtcttcgcc aacctcgtcg
     2821 acttcgacgc gcagttcggg caccgcaacg acgtcgaagg atacgcggcg aatctcgagc
     2881 gcttcgatcg ccgcctggcc cagatgctgc cgctgctccg tcgggatgac ctgctggctc
     2941 tgaccgcgga ccacggtaac gatcccacga cgccgagcac tgaccactcc cgcgagtacg
     3001 tgccgattct gttctacggc gaccaggtgg cgggcggcgt cgatctcggc acgcggtcga
     3061 cgttcgccga cctcggccag acgctggccg gggtcttcgg cgtcgggccg ctcggctgcg
     3121 gcacgagcat gctcggcgac atccggccgg cggcggccta aggactccgg tgcgtcgatg
     3181 agcctgatcc gggaagcgct ggagcggcgg gaggcggaga ccctggcgcc gcaggcggcc
     3241 aagagcgcgc acacgcgcgg ccggctcaat ccggagccgg aagacgacgt gcgcccggcc
     3301 ttccagcgcg accgcgaccg aatcatccat tcgaaggcgt tccgccggct caagcacaag
     3361 acgcaggtct tcttcgcgcc cgagggcgat cactaccgga cgcgcctgac gcactcgctc
     3421 gaggtgtccc agatcgcgcg cacgatcgcc aaggcgctca gcctgaacga ggagctgacc
     3481 gaggccatcg ccctcggcca cgacctcggc catacgccgt tcggccacca gggcgagcgg
     3541 gtgctcgccg aactcgtgcc gggcggattc gaacacgacg tccagagcct gcgcatcgtc
     3601 gacgtgctcg agcgtgacgg cgcgggcctg aacctgacgt gggagacgcg cgacggcatc
     3661 tcccggcact cgaaggggaa gcggggcgcg ccggtgggcc ccgacgtact cgtcaaggcg
     3721 gccaccctcg aagggcagat cgcccgcgcc gccgacctca tcgcctacgt caaccacgac
     3781 atcgacgatg cgatccgggc gaggatcatc ggggccgccg acctgccgcc cgcgcccatc
     3841 ggcgtcctcg gggcgaccca ttccgcgcgc atcgggacgc tggtcaagga cgtggtggtc
     3901 gaaacgcggc aggcgggcct gga
//
LOCUS       DAOSIF010000287         3922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20364_length_3922_cov_3.824412, whole genome shotgun sequence.
ACCESSION   DAOSIF010000287 DAOSIF010000000
VERSION     DAOSIF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20364_length_3922_cov_3.824412"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLT35_09710"
     CDS             <1..563
                     /locus_tag="PLT35_09710"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOG29541.1"
                     /translation="ALVREGRAMPDAAAAILWIAVAFSGTLGLGRTFERERYTDTLRA
                     LMLAPSDRPAVYVGKLLGMLVLLAIVEAALLPLIALFFAAPITASPLWLAALLAAGTL
                     GFASVGTLFAAMLVRARSRDVLLPVLLYPITIPVIIAGVRGTAAILQPVPDEAMARFW
                     AALLVFFDVVFVTLSLWTFEPLMTEQ"
     gene            563..1219
                     /gene="ccsA"
                     /locus_tag="PLT35_09715"
     CDS             563..1219
                     /gene="ccsA"
                     /locus_tag="PLT35_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOG29542.1"
                     /translation="MTRVFAPLACLAMALFAAAPAVVANAPAEATMGLVQRIFYYHVP
                     SAMLMFVSALVCGTASAAYLVRRSAAADRLASAAGELVVVFGLIVLATGPLWARKAWG
                     VWWDWDARLTSSLLLWLMFAAYLLVRRYGGPGSEKLAGAMALFGMANVPFVYVSVNYW
                     RTLHPKTSVIMTLEPGMRSAFWFCVSAFALLYALLLALRLRLEERRAEAEALFLALDE
                     "
     gene            1247..1525
                     /locus_tag="PLT35_09720"
     CDS             1247..1525
                     /locus_tag="PLT35_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HOG29543.1"
                     /translation="MKRLLLALALAALVSGPVLQAWQPAPPQPGTPGDYVPIASLPQA
                     ERLPAAPFLIAAYALVWAVLVAYTWSLWRRITKLEREMKDLGRRLPKA"
     gene            1536..1721
                     /locus_tag="PLT35_09725"
     CDS             1536..1721
                     /locus_tag="PLT35_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29544.1"
                     /translation="MGEMGAGHFIYIPATVLVGIVIGWVLGSRAARDAFAAEQRRRQD
                     ADARAAAKAARGASRQA"
     gene            complement(1744..2481)
                     /locus_tag="PLT35_09730"
     CDS             complement(1744..2481)
                     /locus_tag="PLT35_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG29545.1"
                     /translation="MRYLILSDVHANIDALEQVLADAGRHGAHRVLLLGDLVGYNADP
                     NGVIARIKGLAVEAAVRGNHDRVAAGLGTPANFNAAARASAAATSAMLTQESRDYLRR
                     LPPGPIAVSPLVEICHGTPFDEDEYVFDRLDAVNAIQSASRPLCLFGHTHTPFAMRMT
                     GDEAAFPDVSDGSTFRIEEGNRYLVNVGSVGQPRDGDPRAAYGILDTDGPSLTCYRVA
                     YPVERAGARIREAGLPAGLASRLAVGR"
     gene            complement(2492..2992)
                     /locus_tag="PLT35_09735"
     CDS             complement(2492..2992)
                     /locus_tag="PLT35_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOG29546.1"
                     /translation="MRADKVYIVGFTSAGKTSLARELGRRLGWRVEDVDEGVEARERR
                     SVAAIFAADGEPYFRAIERQVVQGLLGLREAVVATGAGTFVEPDLRALIRADGAVFWI
                     DVPLARLIGRLPQDGSRPLARSRAELAQVFEARRLAYAEAHVRLDGSRATVEELADAV
                     MDWLGA"
     gene            3119..3568
                     /locus_tag="PLT35_09740"
     CDS             3119..3568
                     /locus_tag="PLT35_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29547.1"
                     /translation="MARPAPDTTVRLHFNSAFEMLDLVQMVSDHIGRQLDLDEDALTW
                     MEVAVRESVINAIKHGNHHDRSKYVTVEFTTRRVPPPGELEIAVSDEGEGFDPEALAD
                     PLAPENLLKSSGRGIFFMRSFMDDVRLDRSAAGGMEVHLLKRLAPRA"
     gene            3565..>3922
                     /locus_tag="PLT35_09745"
     CDS             3565..>3922
                     /locus_tag="PLT35_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOG29548.1"
                     /translation="MSSSAASMPLDPRFLATAIDIVLRAGEIQLATLARGLTIGLKGE
                     IDLVTDADLAAERMCRDVLASRFPSHAILAEELGGSVPAPPASGCCWIFDPIDGTTNY
                     AHGLPIFCASLALEIDG"
BASE COUNT          502 a         1416 c         1377 g          627 t
ORIGIN      
        1 tcgccctcgt gcgcgagggc cgggcgatgc ccgacgccgc ggcggccatc ctgtggatcg
       61 ccgtcgcctt ctcgggcacg ctcgggctcg gccggacctt cgagcgcgag cggtacaccg
      121 acacgctgcg cgcgctcatg ctggcgccct cggatcggcc cgccgtctac gtgggcaagc
      181 tcctcggcat gctggtcctg ctggcgatcg tcgaggccgc gctgttgccg ctcatcgccc
      241 tgttcttcgc ggcgccgatc acggccagcc cgctgtggct ggcggcgctg ctggccgcgg
      301 gcaccctcgg cttcgcgtcg gtgggcaccc tgttcgcggc aatgctggtc cgtgcccgga
      361 gccgcgacgt gctgctcccc gtgctcttgt atccgatcac gattccggtc atcatcgccg
      421 gagtccgcgg caccgccgcc atcctgcagc ccgtgcccga cgaggccatg gcgcggttct
      481 gggcggcgct cctcgtgttc ttcgacgtcg tgttcgtcac gctgtcgctg tggacgttcg
      541 agcctctgat gacggagcag tgatgacccg ggtgttcgcg ccgctcgcgt gtctcgccat
      601 ggcgctgttc gccgccgccc cggccgtcgt ggcgaacgcc ccggccgagg cgacaatggg
      661 gctcgtgcag cggatcttct actaccacgt gccgtcggcg atgctcatgt tcgtgtccgc
      721 tctggtgtgc ggcaccgcga gcgcggcgta cctcgttcgc cggtccgccg ccgccgatcg
      781 gctggcctcc gccgccgggg aactcgtggt ggtgttcggc ctgatcgtcc tggcgacggg
      841 ccccttgtgg gcgcggaagg cctggggcgt gtggtgggac tgggacgcgc ggctcacctc
      901 ctcgctgctg ctctggctga tgttcgccgc gtacctgctg gtgcggcggt acggggggcc
      961 gggatcggag aaactcgccg gcgcgatggc gctcttcggc atggccaacg tgccgttcgt
     1021 gtacgtgtcg gtgaactact ggcggacgct ccacccgaag accagcgtca tcatgacgct
     1081 cgaaccaggg atgcgcagcg cgttctggtt ctgcgtgtcc gcgttcgccc tgttgtacgc
     1141 gctcctgctg gcgctgcggc tgcgcctcga agagcggcgc gctgaagcgg aggccctgtt
     1201 cctggcgctc gatgagtgag gccgccgcgc ctcctggaac ctgcgtatga agagactgct
     1261 gctcgcgctc gccctcgccg ccttggtgtc gggcccggtt ctgcaggcct ggcagccggc
     1321 cccgccgcag cccgggacgc cgggggacta tgtgcccatc gcgtcgctcc cgcaggccga
     1381 gcgactcccg gccgctccgt tcctcatcgc ggcctacgcg ctcgtgtggg ccgtgctcgt
     1441 ggcctacacc tggtcgctct ggcgcaggat cacgaagctg gagcgggaga tgaaggacct
     1501 cgggcggcgc ctgccgaagg cgtaaggggg ccgccgtggg ggagatgggg gccggtcatt
     1561 tcatctacat cccggcgacc gtgctcgtcg gcatcgtcat cggctgggtg ctgggctcgc
     1621 gcgcagcccg ggacgcgttt gccgccgagc agcgccggag gcaggacgcc gacgcccgcg
     1681 ccgccgccaa ggccgcgcgc ggcgcgagtc ggcaggcctg aggcgagggg cggagggcgc
     1741 ccgtcagcgg cccacggcca ggcgtgaagc gaggccggcc gggaggcccg cctcgcggat
     1801 gcgcgccccg gcccgttcca ccggataggc gacccggtag caggtgagag aaggcccgtc
     1861 cgtgtcgagg atgccgtagg cggcgcgcgg atcgccgtcg cgcggctggc ccaccgaccc
     1921 cacgttgacg aggtagcgat tcccctcctc gatccggaac gtcgagccat ccgatacgtc
     1981 ggggaaggcg gcctcgtctc ccgtcatgcg catcgcaaac ggcgtatgcg tgtgcccgaa
     2041 caggcagagc ggccggcttg ccgactggat ggcgttgacg gcgtccagcc tgtcgaacac
     2101 gtactcgtct tcgtcgaagg gcgtgccgtg gcagatttcg accagcgggc tcacggcgat
     2161 gggccccggc gggaggcggc gcaggtagtc gcggctctcc tgcgtcagca tggccgaggt
     2221 cgccgcggcg ctggcgcgcg ctgcggcgtt gaagttcgcc ggggttccaa ggccggccgc
     2281 cacgcggtcg tggttgcccc ggacggcagc ttccaccgcc aggcccttga tccgcgcgat
     2341 gacgccgttc gggtcggcgt tgtagccgac gagatcgccc agcaggagca cgcggtgcgc
     2401 accgtgccgc cccgcgtcgg ccagcacctg ctcgagggcg tcgatattcg cgtggacgtc
     2461 gctcaggatc aggtatcgca tgggcggccg cctatgcacc cagccagtcc atgaccgcgt
     2521 cggccagctc ctcgactgtc gctcgggaac cgtcgagccg cacgtgggcc tcggcgtagg
     2581 ccaaacggcg cgcctcgaag acctgcgcca gttcggcccg gctgcgcgcc agcggccggc
     2641 tgccgtcctg cggcagccgg ccgatcagcc gtgcaagcgg cacgtcgatc cagaagaccg
     2701 ccccgtccgc cctgatgagc gcccgcaggt cgggctcgac gaaggtgccc gcgccggtgg
     2761 ccacgaccgc ctcgcgcagc cccagcagcc cctgcacgac ctggcgctcg atggcccgga
     2821 agtacggctc gccgtcggcg gcgaagatgg cggccacgct gcggcgctcg cgggcctcga
     2881 ctccctcgtc cacgtcctcg acccgccagc ccaggcgccg gccgagttca cgggccagcg
     2941 acgtcttgcc cgccgaggtg aacccgacga tgtagacctt gtcggccctc actcgcgcct
     3001 ccccgcccga acgcccctgg cgcgggcccg ccgctcacgt ttgcactata gcatcggctc
     3061 ggatctgggc tatggtatgg ggaacccggc ccggcccccg cctgtggccg gctcatcgat
     3121 ggcgcgacca gcacccgaca ccaccgtccg tctgcacttc aacagcgcct tcgaaatgct
     3181 cgacctggtc caaatggtca gcgaccacat cggccgccag ctggacctcg acgaggacgc
     3241 gctgacctgg atggaagtcg ccgtgcggga gtcggtcatc aacgccatca agcacgggaa
     3301 ccaccacgat cggtcgaagt acgtcaccgt tgaattcacc actcggcggg tgccgccgcc
     3361 cggcgaactc gagatcgccg tcagcgacga aggcgagggc ttcgatcccg aagccttggc
     3421 cgacccgctg gcgcccgaga acctgctcaa gtcgagcggg cggggcatct tcttcatgcg
     3481 cagcttcatg gacgacgtgc gcctcgaccg gagcgcggcg ggaggcatgg aagtccatct
     3541 gctgaagcgc ctcgcgccga gggcgtgagc agcagcgccg cctccatgcc gctcgatccg
     3601 cgtttcctcg ccaccgccat cgacatcgtg ctgcgcgccg gcgagatcca gcttgccacg
     3661 ctcgcccgcg gcctcaccat cgggctgaag ggcgagatcg atctcgtcac cgacgcggac
     3721 ctggcggcgg agcggatgtg ccgcgacgtg ctggcgtcga ggttcccctc gcacgccatc
     3781 ctcgcggagg aattgggagg gtccgtcccg gcgccgccgg cgtcgggctg ctgctggatc
     3841 tttgacccaa tcgatggcac gacgaactac gcccatggcc tgccgatttt ctgcgcctcg
     3901 ctggcgctcg agatcgacgg cc
//
LOCUS       DAOSIF010000288         3911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20424_length_3911_cov_4.003631, whole genome shotgun sequence.
ACCESSION   DAOSIF010000288 DAOSIF010000000
VERSION     DAOSIF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20424_length_3911_cov_4.003631"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLT35_09750"
     CDS             complement(<1..1067)
                     /locus_tag="PLT35_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG29549.1"
                     /translation="MTPAAGCGSGVNRAFVAAVFVLGVAALGDALCSLHRAPVSYLWI
                     ILAVLTIGSDALAAKYEKYLTIPGLVAHVSPSEIFLFLLILLFGGAPAVATVALGGLA
                     FRLARERQRDRSSPWHADKEKALAHAAFNLAEPALSVWVASRCYVWSGGPVPLGASGA
                     SVADVAFPALAMAVAYFVMNGGLNAVAEATSTKQPPLVLWRKYLRDVSVNYFSNASIA
                     VVLAVNLTTAGASAGLRVLVVVAPLVLTPYSSLWLSRRRLEDQAAYVKKMEELQERLV
                     ETLGMTAEAQNPGRSLSHIRRVRKAAVRLARQMGISDPREQRAIAFAGLLHDFGKLKV
                     PEHIQKKPGKLTPDEYEIMKT"
     gene            complement(1064..2305)
                     /locus_tag="PLT35_09755"
     CDS             complement(1064..2305)
                     /locus_tag="PLT35_09755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG29550.1"
                     /translation="MALVQSPLRAGLLRYLGARPYETYDVEALMQTFGRIRVDVVNCL
                     RELVSAGVVRAVSAHPALYQFARPSDPDFARLVEDFLADRPGESAEDRSPAIQRFREM
                     IGRDEKMMVIFESIRTVAKTDLSVLILGPTGSGKEVVARIIHELSSRQHQTFQAVNCA
                     ALPDSLFESEVFGYERGAFTGAFERKPGRIELANHGTLFLDEVGDLSPMAQAKLLRVT
                     EEHRVERLGGRTSIEVDFRLICATNRPLEALVGDGTFREDLYYRLNAFAIRLPALRER
                     PADIPVLADRFLARFCASQGLPLDARRLAPDALQLLVAYPWPGNIRELETTVSRAALS
                     ARDGLVRARDLQFLHPVSAAARPDAPLLVPLRDVERDHIRRVLDALQWNKKRAAQVLQ
                     ISRETLYRKIADFALAQEKRT"
     gene            complement(2713..3744)
                     /locus_tag="PLT35_09760"
     CDS             complement(2713..3744)
                     /locus_tag="PLT35_09760"
                     /inference="COORDINATES: protein motif:HMM:NF039296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HOG29551.1"
                     /translation="MPERDGDARWRAAPARVLGRLARHETLLWWLHSAYVLALGVGIM
                     WLGSRNFAWLRYSGFYLLAIAVLSLLLADVVNVRAGVWWGRARLAVNYVNKNLYQQLL
                     FFILPTYAASATFGSRNVVFVVVLAAAAVTSTLDLVYDRVLSVRRGFAAAFFAFNVFA
                     VVNLALPVMLGTSTKDSLPIAAAASAAGLVVIACRGRRLVWWLGPVAAAALLVFWAAD
                     IVRPFVPPAPLRLEATRFGTSMNRATLLVTEPLPALPEGFAGRVYASTALRAPLGLKE
                     RVLLQWSRDDRLLRSSAGYGVSGGRQGGFRLWSSVAIAPSSGRGPLTLEVVTEAGQLV
                     GRASLPSAR"
BASE COUNT          616 a         1323 c         1437 g          535 t
ORIGIN      
        1 tgcgtcttca tgatctcgta ttcgtcaggg gtgagcttgc ccggcttctt ctgaatgtgc
       61 tcgggcacct tgagcttccc gaaatcgtgc agcagccccg cgaaggcgat cgccctctgc
      121 tcccgggggt ccgagatacc catctgccga gcaagcctga cggcggcctt tctgacgcgg
      181 cggatgtggc tgagggagcg gcccgggttc tgcgcttcgg ccgtcatgcc gagcgtctca
      241 acgagccgct cctggagctc ctccatcttc ttgacgtagg cagcctggtc ttccagccgg
      301 cgccgcgaga gccacagact ggagtagggc gtaaggacca gcggcgccac gacgacgagc
      361 acccggagac cggcgctagc gccggccgtc gtcaggttga cggccaggac gacggcgatg
      421 gaggcgttgc tgaagtagtt gacgctgaca tcgcgcaggt acttgcgcca caggaccagc
      481 ggaggctgct tcgtggaggt ggcttctgcg acggcgttca gcccgccgtt catcacgaag
      541 taggccacgg ccatggcgag cgcgggaaac gcgacgtcgg cgacggaggc gccgctggcc
      601 ccgagcggga cggggccgcc ggaccagacg tagcacctcg aagcgaccca gacggacaag
      661 gccggttcgg cgaggttgaa cgcggcatgg gcaagcgcct tttccttgtc ggcgtgccag
      721 ggcgagctgc gatctcgctg gcgctcccgc gcgagccgga acgccaggcc cccgagcgcc
      781 acggtcgcga cggccggcgc gcccccgaag agcaggatga gcaggaaaag aaagatctcg
      841 gacggcgaca cgtgcgcgac taggccgggg atggtgaggt acttctcgta cttggcggcc
      901 agcgcatccg agccgatcgt cagcaccgcc aggatgatcc acaggtagga caccggtgcg
      961 cggtggagcg agcacagggc gtcccccagg gcggcgacgc cgaggacgaa cacggctgcc
     1021 acgaaggccc tgttcacacc cgaaccgcag ccggcggccg gtgtcacgtg cgcttctcct
     1081 gggcgagggc gaagtcggcg atcttgcggt agagggtctc gcggctgatc tggaggacct
     1141 gggccgcgcg cttcttgttc cactgcagcg cgtcgagcac gcgccggatg tggtcgcgct
     1201 cgacgtcgcg cagcggcacg aggagcggcg cgtccggcct ggcggccgcg ctgacgggat
     1261 ggaggaattg caggtccctc gcccggacga ggccgtcgcg cgccgagagg gccgcgcgcg
     1321 agacggtcgt ctcgagctcg cggatgttgc cgggccacgg gtatgcgacg aggagctgca
     1381 gcgcgtcggg ggcaagccgg cgcgcgtcca aaggcaggcc ctggctggcg cagaacctcg
     1441 cgaggaagcg gtcggcgagg acggggatgt ccgccgggcg ctcgcgcagc gcgggcagcc
     1501 ggatggcgaa cgcgttgagg cggtagtaca ggtcctcgcg gaacgtcccg tcgcccacca
     1561 gcgcctccag cggacggttc gtcgcgcaga tgaggcggaa gtccacctcg atgctcgtcc
     1621 gcccgcccag ccgctcgacg cggtgctcct cggtcacccg gagcagcttc gcctgcgcca
     1681 tcggcgagag gtcgccgacc tcgtcgagga agagcgtgcc gtggttcgcg agctcgatgc
     1741 gcccgggctt gcgctcgaag gcgcccgtga aggcgccgcg ctcgtacccg aagacctccg
     1801 actcgaagag cgagtcgggc agcgccgcgc agttcacagc ctgaaaggtc tggtgctgcc
     1861 ggctgctcag ctcgtggatg atccgggcga cgacttcctt ccccgagccg gtgggcccga
     1921 ggatcagcac ggacaggtcc gtcttggcga cggtgcggat cgactcgaag atgaccatca
     1981 tcttctcgtc gcggccgatc atctcgcgga agcgctggat tgcgggcgac cggtcctctg
     2041 cggattcgcc gggccggtcc gccaggaagt cctcgacgag gcgcgcgaag tcggggtccg
     2101 acggccgcgc gaactggtag agcgccgggt gcgcgctgac ggcgcggacg acgcccgcgc
     2161 tgacgagttc gcgcaggcag ttcacgacgt cgacgcggat gcggccgaac gtctgcatga
     2221 gcgcctcgac gtcgtaggtc tcgtacggcc gcgccccgag gtagcggagc aggccggcgc
     2281 gcagcggcga ttggaccagc gccatgaagc gtgcccggag ctgcgaagcc gtcctggccg
     2341 tctcggctgt cggagcggct cctgacatat gggccgtggt ggtgcgattg agcatccgat
     2401 cgcctcgttc tcgtccaccc ggtgtctcaa gagccgtgcc agacggacga gaacgaggca
     2461 gtcgatggtt tgtggccgga tttcgcgcgt tcgccgcggc gtcgcctcgc gccgcgctac
     2521 gcggagtgtg gcccgcggcc ggccgggcta cacgttgtgt gaggtttcgg ggcacgccgc
     2581 cggaggcgtt cgcctcgcga cggtgcacgc cccgttccgg gcgcgggccg ccgccgcggc
     2641 acgccgcggc aagaggctgt ctggagacgg ggcaggggtg tcagctttcg aggcagagca
     2701 gcggagacgt cgtcagcggg cggacgggag actggcgcgg ccgacgagct ggcccgcctc
     2761 cgtgaccacc tccagcgtga gcgggccgcg ccctgaggac ggcgcgatcg ccaccgaact
     2821 ccagaggcgg aacccgccct gccggccgcc cgacacgccg tagccggccg aggagcggag
     2881 cagcctgtcg tcgcgggacc actgcagcag tacgcgctcc ttcaggccaa gcggcgcgcg
     2941 cagcgccgtc gaggcgtaga cccggcccgc gaagccctcg ggcagcgccg gcaacggctc
     3001 cgtcaccagc agtgtcgcgc ggttcatgct ggtgccgaag cgcgtcgctt ccagcctcag
     3061 cggggcagga ggtacgaagg gacggacgat gtccgccgcc cagaagacca gcagcgccgc
     3121 cgccgctacg ggccccagcc accacaccag gcgccggccc cggcatgcga tcaccaccag
     3181 gccggcggcc gacgcggccg ccgcgatcgg aagcgagtcc ttcgtgctcg ttccgagcat
     3241 gacgggcagc gccaggttca ccacggcgaa cacgttgaac gcgaagaagg cggccgcgaa
     3301 cccgcgccgt accgacagca cccggtcgta gacgagatcg agcgtggacg tcaccgccgc
     3361 ggcggcgagg acgacgacga agaccacgtt acgggagccg aacgtggcgc tggccgcgta
     3421 ggtgggcagg atgaagaaga gcagctgctg atagaggttc ttgttgacgt agttgaccgc
     3481 gaggcgcgcc cggccccacc agacgcccgc ccggacgttg accacgtcgg cgagcagaag
     3541 gctgaggacc gcgatggcga gcaggtagaa gccggaatag cgcagccacg cgaagttgcg
     3601 cgacccgagc cacatgatgc cgacgccgag cgccagcacg tacgcgctgt gaagccacca
     3661 cagcagcgtc tcgtggcgcg ccagccggcc cagtacgcgc gccggcgcgg cgcgccagcg
     3721 cgcgtctccg tcgcgttcgg gcacggccgc ctacttcttg atcggcagcg ggggcggcgg
     3781 cgcaagcttc gccgggttcg tcctcatcag gtccaggatg acctcgatcg ccttgtccag
     3841 ctgctggtcc tcgccggcgt actcacgcgc cgggtcgttg tccacttcga tgtccggctc
     3901 gacgcccacg c
//
LOCUS       DAOSIF010000289         3893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20558_length_3893_cov_3.625847, whole genome shotgun sequence.
ACCESSION   DAOSIF010000289 DAOSIF010000000
VERSION     DAOSIF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20558_length_3893_cov_3.625847"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..689
                     /locus_tag="PLT35_09765"
     CDS             210..689
                     /locus_tag="PLT35_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG29552.1"
                     /translation="MKSMRQIDAMLPLAEVRDLAGEVRRLFDDLDRARPLDRRLLPGV
                     FSPTLDARETADMVEIVLDLPGVALEDIRILIRGGVVIIAGAKSPPNPAERAEASFHL
                     VERDFGRFARAVRLTGAFQTAMTAATLANGELRVRIPKLEERRGHDITVPVRRPAGP"
     gene            718..1536
                     /locus_tag="PLT35_09770"
     CDS             718..1536
                     /locus_tag="PLT35_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOG29553.1"
                     /translation="MRILFIGDVFGRPGRDLVQQAIPLAEARFGVDAVVANVENAAAG
                     AGITRDTGDALLASGVDVMTTGNHVWDKREALTYIGAEPRLLRPLNMAEGAPGRGAFV
                     ARVAGGAAIGVLNVMGRVFMPPVDNPFAAVDAALARLEGACTTIVVDIHAEASSEKIA
                     MAWHLDGRVTAVIGTHTHVQTADARVLPGGTAYITDAGMTGPHDSIIGMERGPLLERF
                     VTGLPGRMEPASANPRFQGVVIDADSRTGRALSIERIDWPETEIAGARKADGDA"
     gene            1529..2974
                     /gene="xseA"
                     /locus_tag="PLT35_09775"
     CDS             1529..2974
                     /gene="xseA"
                     /locus_tag="PLT35_09775"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG29554.1"
                     /translation="MPDLFDFLPESGGADGAPPRDQGPRIYTVGEITAAIRTVLETTW
                     ADVWIEGELSNCRLWNTGHLYFSLKDPSAQLRGVMFKREARSLAFRPEDGLHVLVRGR
                     LGVYEPKGEYQIVCSVMEPRGLGARQLALDQLKRRLQAEGLLDAARKRPLPALPRKIG
                     VVTSMDGAALRDVLTVLGRRYPDAHVVIAPARVQGEGAAADLARALGAIGRVDGVDVV
                     IVGRGGGAVEDLWAFNEEPVARAVAQCPVPVVSAVGHEVDVTLADLVADLRAPTPSAA
                     AELVVAAKDEVVRRIDHLADRLDAACGRRVRLAAHALDRLTGRPAFAGFAARLALRGR
                     HVAELLLDLQRAGRDRIAARQRGFQALRLRLEALDLRRSLGRLQARLAQGHARLASAF
                     AAHRHAADARLGALTARLDALSPLAVLGRGYAVCWDGARRLILRDASAVADGTAVRVT
                     LHRGELSCVVTGRDVPEPAPHTARARVQDGP"
     gene            2974..3258
                     /gene="xseB"
                     /locus_tag="PLT35_09780"
     CDS             2974..3258
                     /gene="xseB"
                     /locus_tag="PLT35_09780"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOG29555.1"
                     /translation="MDDHIKDFEAAIAELEAIVKKLEDGDLTLEQSLELYERGVRLSR
                     YCHGRLEEAERRIEVLGERGDLQPAPPSLDAASAGARRAEAGPGEKGRRT"
     gene            3255..>3893
                     /locus_tag="PLT35_09785"
     CDS             3255..>3893
                     /locus_tag="PLT35_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG29556.1"
                     /translation="MTDTSGLPFASFLRTRRAEIDRALDAFLPPVSGRLAGVHDAMRY
                     SLFAGGKRFRPILALAAAESAAPGDPAALDLALPSACAIEMIHTYSLVHDDLPAMDDD
                     TMRRGKPTLHVVCGEGLAILAGDGLLAEAFVLLAREPDCEGRPERAARKLAVIRRIAD
                     AAGAAGMVGGQAIDLQAAGRGCDAAGLDAEGLRDMHARKTGALIRAAAASGAI"
BASE COUNT          479 a         1460 c         1465 g          489 t
ORIGIN      
        1 gcgggcgccg tgcggaacct cacgcgcggg cgggagttcg cgacggtgcc gctgccgccg
       61 gcgaggcagg caatcatcga cgccggcggg ctgattgcct acacgcgcgc gcggctgctg
      121 gcggccgccg gcagataggc gcgttggccg ctgcggcgcc gcacggcggg atcccgcccg
      181 cctagcgggg cctccgggcc tgtgcgatca tgaagagcat gcgccagatc gacgcgatgc
      241 tgccgcttgc cgaggtgcgg gacctcgccg gcgaggtgcg ccgcctcttc gacgatctcg
      301 accgggcgcg ccccctcgac cggcgcctgc tgccgggcgt gttctcgccg acgctcgacg
      361 cgcgggagac ggcggacatg gtcgagatcg tcctggatct gcccggcgtc gcgctcgagg
      421 acatccgcat cctgatccgc gggggcgtcg tcatcatcgc cggcgcgaag tcgccgccca
      481 acccggccga gcgcgccgag gcgagcttcc acctggtgga gcgcgatttc ggccggttcg
      541 cgagggcggt gcgcctgacc ggcgccttcc agaccgcgat gacggccgcc acgctggcca
      601 acggcgaact tcgcgtgcgc attccgaaac tcgaggagcg gcgcgggcac gacatcaccg
      661 tgccggtcag gcgcccggcc gggccctagg ccgcccgggg cgccgcgcgc ggtcaccatg
      721 cggatcctgt tcatcggcga cgtgttcggg cggcccgggc gggacctcgt ccagcaggcg
      781 atcccgctcg ctgaggcccg cttcggcgtg gacgccgtcg tggccaacgt cgagaacgcg
      841 gcggcgggcg cgggcatcac ccgcgacacg ggcgacgcgc tcctggcgtc gggcgtggac
      901 gtgatgacca cgggcaacca cgtctgggac aaacgcgagg cgctgaccta catcggcgcc
      961 gagccccggc tgctgaggcc gctcaacatg gccgaggggg cgcccggccg cggcgccttc
     1021 gtcgcccgcg tcgcgggcgg cgccgcgatc ggcgtcctca acgtcatggg gcgcgtgttc
     1081 atgccgccgg tggacaatcc cttcgcggcg gtggacgccg ccctggcgcg cctcgaaggg
     1141 gcctgcacca cgatcgtcgt ggacatccac gccgaggcct cctcggagaa gatcgcgatg
     1201 gcctggcacc tcgacggccg cgtgaccgcc gtcatcggca cgcacacgca cgtccagacg
     1261 gccgacgcgc gggtgctgcc gggcggcacg gcctacatca ccgacgccgg catgacgggc
     1321 ccccacgact cgatcatcgg catggagcgc gggccgctgc tcgagcgatt cgtcaccggc
     1381 ctgccgggcc gcatggagcc cgcgtccgcc aatccccggt tccagggcgt cgtgatcgac
     1441 gccgacagcc ggaccggccg cgcgttgtcg attgagcgca tcgactggcc ggagacggag
     1501 atcgcgggcg cccgcaaggc cgacggcgat gcctgatctc ttcgacttcc tgcccgagag
     1561 cggcggcgcg gacggcgcgc cgccacgcga ccagggcccg cggatctaca cggtcggcga
     1621 aatcacggcc gccatccgca ccgtcctcga gaccacctgg gccgatgtct ggatcgaggg
     1681 cgagctgtcg aactgccgcc tctggaacac ggggcacctg tacttctcgc tcaaggaccc
     1741 ttcggcccag ctgcgcggcg tgatgttcaa gcgcgaggcc cgctccctcg ccttcaggcc
     1801 ggaagacggc ctccacgtgc tcgtgcgcgg ccggctgggc gtgtacgagc cgaagggcga
     1861 gtaccagatc gtctgcagcg tgatggagcc gcgcgggctg ggcgcgcggc agctggcgct
     1921 cgaccagctc aagcgccgcc tgcaggccga ggggctgctc gacgcggccc gcaagcggcc
     1981 gctcccggcg ctgccccgca agatcggcgt cgtgacctcg atggacggcg ccgcgctgcg
     2041 cgacgtcctc acggtcctcg gccggcggta cccggacgcc cacgtggtga ttgcgcccgc
     2101 gcgggtgcag ggcgaggggg ccgcggccga cctcgcccgc gcccttggcg cgatcgggcg
     2161 cgtcgacggc gtggacgtcg tgatcgtcgg gcgcggcggc ggggccgtcg aggacctgtg
     2221 ggccttcaac gaggagccgg tggcgcgggc ggtggcacag tgccccgtgc ccgtcgtctc
     2281 ggccgtgggc cacgaagtgg acgtgacgct cgccgacctg gtcgcggacc ttcgggcgcc
     2341 gacgccgtcg gcggcggccg agctggtggt ggcggcgaaa gacgaggtcg tccggcgcat
     2401 cgatcacctc gcggaccgcc tggacgccgc ctgcgggcgg cgggtgcggc tggccgcgca
     2461 cgcgctggat cgcctcacgg ggcgcccggc cttcgcgggg ttcgcggcgc ggctcgcgct
     2521 gcgcgggcgc cacgtcgccg agctgctgct cgatctccag cgcgccgggc gcgaccggat
     2581 cgccgcgcgg cagcgggggt tccaggcgct gcgcctgcgc ctcgaggcgc tggacttgcg
     2641 ccgcagcctc ggccggctgc aggcgcgcct cgcgcagggc cacgcgcgcc tcgcctcggc
     2701 cttcgccgcg catcgccacg cggccgacgc ccggctgggc gccctgacgg cccgcctcga
     2761 cgcgctgagc cccctggcgg tcctcgggcg cggctacgcc gtctgctggg acggcgcgcg
     2821 ccgcctgatt ctccgtgacg cgagcgccgt cgccgacggg acggccgtcc gcgtcacgct
     2881 tcaccggggc gagttgagct gcgtcgtgac gggccgggac gtcccggagc cggcgccgca
     2941 cacggcacgc gcgcgcgtgc aggatggacc gtgatggacg atcacatcaa ggacttcgaa
     3001 gccgccatcg ccgagctgga agccatcgtg aagaagctcg aggacggcga cctgaccctc
     3061 gagcagtcgc tcgaactcta cgagcgcggc gtgaggctct cccgctactg ccacgggagg
     3121 ctcgaagaag cagagcgccg gatcgaggtg ctgggcgaac ggggcgacct gcagccggcg
     3181 ccgccgtcgc tcgatgccgc gagcgccggc gcccgccggg ccgaggccgg ccccggggag
     3241 aaggggcgca ggacgtgacc gacacctccg gcctgccttt cgccagcttc ctgcgcacgc
     3301 ggcgcgcgga gatcgatcgg gccctcgacg ccttcctccc gcccgtctcg ggccgcctgg
     3361 cgggcgtgca cgacgccatg cgctacagcc tcttcgccgg cggcaagcgc ttccggccga
     3421 tcctcgccct cgccgcggcc gagtcggccg cgcccggcga cccggccgcg ctcgatctcg
     3481 cgctgccctc cgcgtgcgcg atcgagatga tccacaccta ttcgctcgtc cacgacgatc
     3541 tcccggccat ggacgacgac acgatgcggc gcggcaagcc cacgctgcac gtggtctgcg
     3601 gcgaaggcct cgccatcctg gccggcgacg ggttgctggc cgaggccttc gtgctgctgg
     3661 cgcgcgaacc ggactgcgag gggcggcccg aacgcgccgc gcgcaagctg gcggtcatcc
     3721 gccgcatcgc ggacgcggcg ggcgctgcgg gcatggtcgg cgggcaggcg atcgacctgc
     3781 aggccgccgg ccgcggctgc gacgccgcgg ggctggacgc cgagggcctc cgggacatgc
     3841 acgcgcgcaa gaccggcgcg ctcatccgcg ccgcggcggc ctcgggcgcc atc
//
LOCUS       DAOSIF010000290         3884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20623_length_3884_cov_3.638548, whole genome shotgun sequence.
ACCESSION   DAOSIF010000290 DAOSIF010000000
VERSION     DAOSIF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20623_length_3884_cov_3.638548"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /gene="tsaB"
                     /locus_tag="PLT35_09790"
     CDS             <1..736
                     /gene="tsaB"
                     /locus_tag="PLT35_09790"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOG29557.1"
                     /translation="PMIVLALDTTTRGGSIAVARDGVVCSVLTGDPARPHAERLPGDL
                     TRALAGAGLSLAQVDVFAVAAGPGSFTGLRIGIAAIQGCAFASGKPVIGVSALEALAL
                     AAPLNSGTAMPSTLGVWMDAQRQEVFSELFARDSRADPARLEVLEGPQVGAPRDVADR
                     WRSAHGGLRFPVVGDGAVAYRGLLLEAAGGAVEIIAPPPLAPTIAVMAWRRAAAGERP
                     LPHAVRPLYVRRPDAELARDRAGGGA"
     gene            733..1215
                     /gene="rimI"
                     /locus_tag="PLT35_09795"
     CDS             733..1215
                     /gene="rimI"
                     /locus_tag="PLT35_09795"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOG29558.1"
                     /translation="MSPARHIEWLRGADDLALVLEIDRESFAAPWTRAMYEQELEDAS
                     HAFIAVLRLPGQPVAAYCSYRLVGGELQINNVAVRRALRRRGLGRELVTFVLRHGADK
                     GAEAAYLEVRPSNEAARRLYAALGFVPAGTRPRYYSNPEEDALVLVRRPLDAAPDPAP
                     "
     gene            1367..1615
                     /locus_tag="PLT35_09800"
     CDS             1367..1615
                     /locus_tag="PLT35_09800"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOG29559.1"
                     /translation="MTVHGQDHRAMLLQTDDEYRQLAARHKELEQRLAELASKAHLSE
                     PEHIEQVTIKKRKLQLKDRMEDIVRRSSREADRTARPS"
     gene            1752..2378
                     /locus_tag="PLT35_09805"
     CDS             1752..2378
                     /locus_tag="PLT35_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOG29560.1"
                     /translation="MKIDRAGWPFVFAFLVPAAACVLAGWIPAAAMAALGAGSMLFFF
                     RDPERRTPGEPEAVVSPADGRVLVAGRADPLTSPPPGEWTQLSIFLSPLDVHVNRIPI
                     GGRVVKVERTPGRFLAAYRPDSARVNEKNDVWIERGSETVVCRQVVGVLARRLVCRVG
                     PGSEVATGQRFGLMKFGSRIDLYLPPRIVLCVAEGARVRGGETVVARW"
     gene            2372..3193
                     /gene="pssA"
                     /locus_tag="PLT35_09810"
     CDS             2372..3193
                     /gene="pssA"
                     /locus_tag="PLT35_09810"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOG29561.1"
                     /translation="MVTPEYDANGAAGARQRLKALDPRRWQNGRSPGLRRGAYLLPSL
                     LTLGNMLCGYLAVLFAIRGEVGTSAPLIAVAVVLDMLDGRVARLTGTATAFGVEFDSL
                     ADVISFGIAPAVLVFMWGLQPLHRLGWAAGFIFLSAAALRLARFNIQTTKSADKRYFV
                     GMPSPAAAAVPVSTVYFYQPGFQDARWALIALAVTVAPALLMVSTIRFNSFKTIDNPQ
                     RRSYKVLALSALVLAVILLHPSIVLLVLAYTYMLSGPAGLLWARFKRRGAPTPSR"
     gene            complement(3185..>3884)
                     /locus_tag="PLT35_09815"
     CDS             complement(3185..>3884)
                     /locus_tag="PLT35_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29562.1"
                     /translation="LEAWADMARQVAHEIKNPLTPIQLSAEHLRRVHLDRGLPLSPVL
                     EDCVESILAQVRLLRQIAGEFSSFATAPTPRPVVTAPADLVDEVVAPYRAGLPAAVSL
                     AVNVPAGLPALNVDRSLVGRALTNVIENSIHAMPSGGTIAISGSAAAGGTAVELRIAD
                     TGVGMDESALRRIFEPYFSTKAVGTGLGLSIAKRNVELHGGTIEAESTPAAGTTVTVR
                     LPAGGAPPEHGGSA"
BASE COUNT          527 a         1332 c         1424 g          601 t
ORIGIN      
        1 ccccatgatc gtgctggcgc tcgacacgac gacacgaggc ggaagcatcg ccgtagcgcg
       61 cgacggcgtc gtctgtagcg tcctgaccgg cgaccccgcg cgcccgcacg ccgagcgtct
      121 cccgggtgac ttgacccgcg cgctggcggg agccgggctg tcgctcgcgc aggtcgacgt
      181 gttcgccgtg gctgctggcc ccgggtcctt caccgggctc cgcatcggca tcgcggcaat
      241 ccagggctgc gcgttcgcgt cgggcaagcc tgtcatcggg gtctcagcgc tggaagcgct
      301 cgcgctggcg gcgccgctca acagcgggac tgcgatgccc tcgacgctgg gtgtctggat
      361 ggacgcgcag cgccaggagg tcttctcgga gctcttcgcg cgggacagcc gcgccgaccc
      421 ggctcggctg gaagtcctcg aagggccgca ggtgggcgct cctcgagacg tggcggaccg
      481 ctggcggtcg gcgcacggcg gcttgcgctt tcccgtcgtc ggcgacggcg ccgtggcata
      541 ccgcggcctg ctgctcgagg ccgctggggg ggcggtcgag atcatcgcgc cgccacccct
      601 ggccccgacc atcgcggtga tggcctggcg gcgggctgcg gcgggcgagc ggccgctgcc
      661 gcacgccgtc aggccgttgt acgtccggcg cccggacgcg gaactggccc gagaccgagc
      721 gggaggaggg gcgtgagccc ggcgaggcac atcgagtggc tccggggggc tgacgatctc
      781 gccctcgtcc tcgagatcga ccgggagagc tttgcggccc cgtggacgcg agcgatgtac
      841 gagcaggagt tggaggacgc gagccacgcc ttcatcgccg tcctccggct gcccggccag
      901 cccgtcgcag cctactgttc gtaccgcctg gttggtggcg agctccagat caacaacgtg
      961 gcggtccggc gcgcgctcag gcggaggggc cttggccgtg agctcgtcac gttcgtcctc
     1021 cgtcacgggg cggacaaggg ggcagaggcc gcttacctgg aggtgaggcc ttcgaacgag
     1081 gcggccaggc gcctgtacgc cgcactggga ttcgttccag ccgggacaag gccgcgctac
     1141 tactccaacc cggaggaaga cgccctggtg ctggtgcggc ggcccctgga cgccgcaccc
     1201 gatccggcgc cttgaatcgc ctggatccgt gtgctaccgt taacctaagc ggcacgcgcc
     1261 agagccggcg ggtcgttttc cggctcgtcg gctggtccga catcatgttc ctccacaggc
     1321 tgactcacgc acgcaccgta gtccccgtca caaggaggcg gaaagcatga ctgtacacgg
     1381 gcaggaccac cgcgccatgc ttctgcagac cgacgacgaa taccggcagc tggccgcgcg
     1441 ccacaaggag ctcgagcagc ggctggccga gctcgcgagc aaggcccacc tctccgaacc
     1501 cgaacacatc gagcaagtca ccatcaagaa gcgcaagctc cagctgaagg atcggatgga
     1561 ggacatcgtc cggcgctctt ctcgcgaggc cgatcgaacg gcgcggccgt cctagtcgcg
     1621 gtccgtggcg gcgggtgagg ccgccagccc cgatgcccgg cagccagggt cccgccgcgg
     1681 gccgctggct gttcgcgttt ccggcgatca gcgccgcctg cgcctgcggg ccacgataga
     1741 ataggcgcgt catgaagatc gatcgggccg gctggccgtt cgtgttcgcg ttcctggtgc
     1801 cggcagccgc gtgcgtcctg gcaggctgga tcccggcggc cgcgatggcg gcgctgggcg
     1861 ccggctccat gctcttcttc ttccgggatc ccgaacgccg gacgcccggc gagcccgagg
     1921 ccgtcgtgtc gccggccgat ggcagggtcc tcgtggcggg ccgggcggat ccgctcacct
     1981 cgccgccgcc gggagagtgg acgcagctca gcatcttcct ctcgcccctc gacgtgcacg
     2041 tgaaccggat tcccatcggg gggcgcgtgg tgaaagtgga acgcacgccc gggcggtttc
     2101 tggcggcgta ccgcccggac tcagcgcggg tcaacgagaa gaacgacgtg tggatcgagc
     2161 gcggcagcga gacggtggtg tgtcgccagg tggtgggggt gctggccagg cggctggtct
     2221 gccgggtggg ccccggctcg gaggttgcga cgggccagcg cttcggcctg atgaaattcg
     2281 gctcgcggat tgacttgtac ctgcctccgc ggatcgtgct ttgcgtggca gaaggcgccc
     2341 gggtcagggg cggtgaaacg gttgtggcga gatggtgacg cctgaatacg atgcgaatgg
     2401 cgcggccggg gcgcgccagc gcctcaaggc gctcgacccg cggcggtggc agaacggaag
     2461 aagccccggt ctgcggcgcg gcgcctacct gctgcccagc ctgctgacgc tcggcaacat
     2521 gttgtgcggc tacctcgccg tgctgttcgc gatcaggggc gaggtcggca cgtccgcccc
     2581 gctcattgcc gtggcggtcg tgctggacat gctcgacggc cgggtggcgc ggctgacggg
     2641 gacggcgacg gcgttcgggg tcgagttcga ttcgctggcc gacgtcatct cgttcggcat
     2701 cgcgccggcg gtccttgtct tcatgtgggg gctccagccg ctgcaccggc tgggctgggc
     2761 cgcgggcttc atcttcctga gcgccgcggc cctgcgcctg gcccgcttca acatccagac
     2821 gacgaagagc gccgacaagc ggtacttcgt cggcatgccg agcccggcgg cggcggcggt
     2881 gccggtgtcg acggtctact tctatcagcc gggatttcag gacgcccggt gggcgctcat
     2941 cgcgctggcg gtcaccgtcg cgccggcgct cctcatggtc agcacaatcc ggttcaacag
     3001 cttcaagacg atcgacaatc cgcagcgccg gtcctacaag gtgctcgccc tgtccgcgct
     3061 cgtcctcgcg gtcatcctgc tgcacccgag catcgtgctg ctggtgctgg cgtacaccta
     3121 catgctctcg gggccggccg gtctgctctg ggctcggttc aagcgccgcg gcgctcccac
     3181 gccgtcacgc tgagccgccg tgctccgggg gggcaccgcc ggcgggcagc cgcacggtga
     3241 ccgtggtgcc cgcggccggc gtgctctcgg cctcgatcgt ccctccgtgc agctcgacgt
     3301 tgcgcttcgc aatcgagagc ccgagcccgg ttccgacggc cttcgtggag aagtacggct
     3361 cgaagatgcg ccggagagcg ctctcgtcca tgcccacgcc ggtgtccgcg atccgcagtt
     3421 cgacggcagt cccgccggcg gctgccgagc ccgagatcgc gatcgtcccg ccggagggca
     3481 tcgcgtgaat ggagttctcg atgacgttcg tcagggcccg gccgacgagc gagcggtcca
     3541 cattcagcgc gggcagaccg gcaggcacat tcaccgcaag ggacacggca gccggcaatc
     3601 cggcccggta cggagcgacg acctcgtcga cgaggtcggc gggggccgtg acgacgggcc
     3661 gtggggttgg cgccgtcgcg aagctcgaga actcgccggc gatctgccgg agcaggcgga
     3721 cctgcgccag gatcgattcg acgcagtcct cgagcacggg cgagagcggc aggccccggt
     3781 ccaagtggac gcggcgcagg tgctcggccg agagctggat cggcgtgagc gggttcttga
     3841 tctcgtgcgc cacctggcgg gccatgtcgg cccacgcttc gagc
//
LOCUS       DAOSIF010000291         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20662_length_3878_cov_3.500392, whole genome shotgun sequence.
ACCESSION   DAOSIF010000291 DAOSIF010000000
VERSION     DAOSIF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20662_length_3878_cov_3.500392"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLT35_09820"
     CDS             complement(<1..495)
                     /locus_tag="PLT35_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29563.1"
                     /translation="MTDRLGLGLTAAASAILALAVSTAAVQPAGKGARVDKTAFGTTR
                     EGQPVDLYTLVNANGVTAKLITYGALLTELHVPDRTGQLADVVLGFRTLAEYEGGHPY
                     FGATIGRVGNRIANAKFRLDGRDYALAANDGPNHLHGGLRGFDKRVWKGQVVPAPSGA
                     AVRFT"
     gene            complement(492..1061)
                     /locus_tag="PLT35_09825"
     CDS             complement(492..1061)
                     /locus_tag="PLT35_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG29564.1"
                     /translation="MIDRRRWAHGESDGAAAPQELLEKPSYVQELEARAAAAEAELRS
                     TLARYREASAEFEQAKVRIRREVAKDVERGSRDLLAGLLDVVDNLDRAIEAGRSSEPG
                     SPLLHGVEMVRAQFLAALEARGVRPMAALGLPFDPAAHDAVTAVPAAEQGQDGLVVGV
                     VRQGYTIGEDVLRPAVVAVAAWNAAGAPT"
     gene            complement(1124..3475)
                     /locus_tag="PLT35_09830"
     CDS             complement(1124..3475)
                     /locus_tag="PLT35_09830"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG29565.1"
                     /translation="MFSSSVELLLNVTYREAVSRRHAHLTLEHLLYVMAHDVEGEKIL
                     AACGADLPRLRRELDTFLRERIEQLPPGIDKQPEQTLAFRRALQTAVLHVQSAGRDEA
                     RAGDVLAALVQQSRSYAATLLASHGITRLDILNYISHGISKVPQGPGAQQPKLGPDPD
                     GGGTPRPGEDEQPAPAADPLSAYTVNLTERAAAGKLDPLVGRTTELNRTLEILCRRRK
                     NNPVFVGDAGVGKTALAEGLAQRLLDEDVPGILKGAEIHSLDTAALLAGTRFRGDFEE
                     RFKAVIGALLAKPLPILFIDEIHATVGAGATTGGTLDLATLIKPVLTAGDLRILGSTT
                     FEEFKHIEKDRALARRLQKVVVDEPSVEETVLILKGLKSRYEAHHQVRYADDALDAAA
                     RLAKRHLRDLRLPDSAIDLIDEAGARLRMRPADGGPPDAERLVSTDDIEIVAARMARI
                     PEKQASVSDRERLRTLEESLQRVVFGQEDAVRQVAAAIKRSRAGLGQPDRPAGCFLFT
                     GPTGVGKTELARQLAIHLGNEFIRYDMSEYMEKHAVARLIGAPPGYIGFEQGGLLVDA
                     VRTHPYAVVLMDEIEKAHADVYNILLQVMDHATLTDNTGRKADFRQVILILTSNAGSR
                     EMSAGTIGFSGGHAASTRAKAAKLRAKEAVERIFSPEFRNRLDAIVSFQALSSAIMES
                     IVEKFILQLEAQLRERRVAIVLAPAARAWLAAKGYDPIYGARPLARVIQAEVRDRLTD
                     EVLFGKLEHGGTALIGADGDTLTFTFDPAPPAPAEQPPALAGA"
     gene            complement(3516..>3878)
                     /locus_tag="PLT35_09835"
     CDS             complement(3516..>3878)
                     /locus_tag="PLT35_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOG29566.1"
                     /translation="HCTRSAAASPTVYWQPMADDRPETGRSVSERTRAEPREPDLYHV
                     KLLNDDYTTMAFVVAVLEAVFDKRPAEAYRIMMQVHTEGQAVCGTYPWDIAETKVAAT
                     HALAEDQGFPLRAVTELA"
BASE COUNT          504 a         1366 c         1374 g          634 t
ORIGIN      
        1 ggtgaacctg accgccgcgc cggacggcgc cggcacgacc tgtcccttcc agactcgttt
       61 gtcgaatccg cggaggcccc cgtgcaagtg gttcgggccg tcgttcgccg cgagcgcgta
      121 gtcgcggccg tcgagcctga acttcgcgtt cgcgatccgg ttcccgacgc ggccgatcgt
      181 cgcgccgaag tacggatggc cgccttcgta ctccgccagg gtcctgaagc cgagcacgac
      241 gtccgccagc tggccggtcc ggtcgggcac atggagctcc gtgagcagcg ccccgtacgt
      301 gatgagcttg gcggtgacgc cgttggcgtt caccaacgta tagagatcga cgggctgccc
      361 ctccctggtc gttccaaacg ccgtcttgtc cacgcgggcc cccttgccgg ccggctgcac
      421 ggcagccgtc gacacggcca gcgccaggat ggcgctggcg gcggccgtca ggccgagtcc
      481 gagccgatct gtcatgttgg cgctcccgct gcgttccagg ccgcgacggc gaccaccgcc
      541 ggccgcagca cgtcctcgcc gatggtgtac ccctggcgga cgacgccgac gacgagcccg
      601 tcctggccct gttccgctgc ggggacggcg gtcacggcgt cgtgtgcggc cgggtcgaac
      661 gggaggccca gcgcggccat cggccgcacg ccgcgcgcct cgagggccgc caggaactgc
      721 gcgcgcacca tctcgacgcc gtggagcagg gggcttcccg gctcgctcga gcggccggcc
      781 tcgatggccc ggtcgaggtt gtccaccacg tcgagcaagc cggccagcag gtctcgggag
      841 ccccgttcga cgtcctttgc cacctcccgg cggatccgga ccttcgcctg ctcgaactcg
      901 gcgctggcct ctcggtaccg cgcgagcgtg ctgcgcaact cggcctcggc cgcggccgct
      961 cgggcctcga gctcctgaac gtagctgggc ttctcgagca gctcctgcgg agccgccgcg
     1021 ccgtcggact ctccgtgagc ccacctgcgc ctgtcaatca ccgggattcc aggttctgcc
     1081 tcgcctgtca tcgcctgctg gctccgggcg acccggtccg cgcctacgcg ccggcgagcg
     1141 ccggcggctg ttcagccggc gcggggggag ccgggtcgaa ggtgaacgtg agcgtgtcgc
     1201 cgtcggcccc aatcagggcc gtgccgccgt gttcgagctt gccgaagagg acctcgtctg
     1261 tcagccggtc gcgcacctcc gcctggatga cgcgcgcaag gggcctggcc ccgtagatcg
     1321 ggtcgtagcc cttcgccgcc agccaggcgc gggccgccgg cgcgagcacg atcgcgaccc
     1381 gccgctcgcg caactgcgcc tcgagctgca ggatgaactt ctcgacgatg gactccatga
     1441 tggcggacga cagcgcctgg aagctcacga tggcgtcgag gcggttgcgg aactccgggc
     1501 tgaagatccg ctccaccgcc tccttcgcgc gcagcttggc ggctttcgcc cgggtcgagg
     1561 cggcgtggcc cccagagaaa ccaatggtgc ccgcgctcat ctcgcgcgag cccgcattcg
     1621 acgtcaggat caggatcacc tggcggaagt ccgccttgcg gcccgtgttg tcggtcagcg
     1681 tggcgtgatc catcacctgc aacaggatgt tgtagacgtc cgcgtgggcc ttctcgatct
     1741 cgtccatgag caccacggcg tacgggtgcg tccggacggc gtccacgagg aggccgccct
     1801 gctcgaatcc gatgtacccg ggcggcgcgc cgatcaggcg ggccaccgcg tgcttctcca
     1861 tgtactcgga catgtcgtag cggatgaact cgttgccgag gtggatggcg agctggcggg
     1921 cgagttccgt cttgcccacc cccgtcgggc cggtgaacag gaagcaccca gccgggcggt
     1981 cgggctggcc caggccggcg cgtgaccgct tgatggccgc cgcgacctgc cgcaccgcgt
     2041 cttcctggcc gaacaccacg cgctgaaggg actcctcgag ggtgcgaagc cgctcccggt
     2101 cggagacgct cgcctgcttc tccgggatcc gggccatgcg cgcggccacg atctcgatgt
     2161 cgtccgtcga aacgaggcgc tcggcgtcgg gtgggccgcc gtccgccggc cgcatgcgga
     2221 gccgggcgcc ggcctcgtcg atcaggtcga tggcgctgtc ggggagcctg aggtcgcgca
     2281 ggtggcgctt ggcgagcctc gcggcggcgt cgagcgcgtc gtcggcgtag cggacctggt
     2341 ggtgggcctc gtagcggctc ttgaggccct tcaggatgag gacggtttcc tccaccgacg
     2401 gctcgtcgac cacgactttc tgcagccggc gggcgagggc ccggtccttt tcgatgtgct
     2461 tgaactcctc gaatgtcgtc gagccgagga tgcgcaagtc gcccgcggtc aggaccggct
     2521 tgatgagcgt cgccagatcg agcgtgccgc ccgtcgtcgc gccggcgccg acggtggcgt
     2581 gaatctcgtc gatgaagagg atcggcagcg gcttggccag cagggccccg atgaccgcct
     2641 tgaagcgctc ttcgaagtcg ccgcggaagc gcgtccccgc gaggagggcg gcggtgtcga
     2701 gggaatggat ctccgctccc ttcaggatgc cgggcacgtc ctcgtcgagc agccgctgcg
     2761 ccagcccttc cgcgagcgcc gtcttgccga cgccggcgtc gccgacgaac accgggttgt
     2821 ttttccgccg gcggcagagg atctcgagcg tgcggttgag ttccgtcgtt cgtcccacga
     2881 gcgggtcgag tttgccggcc gcggcccgct ccgtcaggtt cacggtgtag gccgagagcg
     2941 ggtcggccgc cggggccggc tgctcgtcct cgcccggcct cggcgtgccg ccgccgtccg
     3001 ggtccggacc gagcttcggc tgctgcgccc cgggcccctg gggcaccttg ctgatgccgt
     3061 gggagatgta gttcaggata tcgaggcgcg taatgccgtg cgacgcgagc agcgtggccg
     3121 cgtacgagcg ggactgctgg acgagggcgg caaggacgtc accggcacgt gcttcgtccc
     3181 ggcccgcgct ctgcacgtgg agcaccgccg tttggagggc ccggcggaag gccagcgtct
     3241 gctcgggctg cttgtcgatc cccggcggaa gctgctcgat gcgctcccgc aggaaggtgt
     3301 cgagctcgcg ccggaggcgg ggcaggtcgg ccccgcacgc ggcgaggatc ttctcgccct
     3361 cgacgtcatg agccatgacg tagagcaggt gctcgagcgt caggtgcgcg tggcggcgag
     3421 acaccgcctc gcggtacgtc acgttgagca gcaattcgac cgacgagctg aacacgggcg
     3481 gcgtcctttc acgatccccg cttccagtat accgttcacg ccagttccgt gaccgccctg
     3541 agcgggaacc cctggtcctc ggcgagcgca tgagtggcag ccaccttcgt ctcggcgatg
     3601 tcccacgggt aggtcccgca caccgcctgc ccctccgtat gcacctgcat catgatgcgg
     3661 taggcttcgg ccggccgctt gtcgaacacg gcctcgagca cggcgaccac gaacgccatc
     3721 gtcgtgtagt cgtcgttcag cagcttgacg tggtacaggt ccggttcacg cggttcggcg
     3781 cgggtcctct cggatacgga ccggccagtc tcggggcgat cgtcggccat cggctgccag
     3841 tataccgtcg ggctggccgc ggcgcttcgg gtacaatg
//
LOCUS       DAOSIF010000292         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20682_length_3875_cov_3.788482, whole genome shotgun sequence.
ACCESSION   DAOSIF010000292 DAOSIF010000000
VERSION     DAOSIF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20682_length_3875_cov_3.788482"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1339)
                     /locus_tag="PLT35_09840"
     CDS             complement(17..1339)
                     /locus_tag="PLT35_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOG29567.1"
                     /translation="MSASPARRLRWAEIIAVGTELLAPPRLDTNSLVITRRLNDLGIE
                     IRAKTIVGDRADDLRSAVRGAMARTELVVLCGGLGPTDDDLTRDVVADVTSRAMSEDP
                     GIVDEIRRRFEARGARMPERNRRQALVPDGAVVLPNAAGTAPGLWIEHEGSILVLLPG
                     PPAELTPMLESVVEGRLASLAGDERLHRRVLRVCGRTESETDEAVAPVYSQWLHEDPP
                     VETTILSSPGQVELDLTLRSASAARASARLEAAVGALTAILGADVYSTDGRTIEETVG
                     GLLRARGWRIGVAESCTGGLVSARLTEVPGSSDYVGLNVVCYSNEAKAAHLGVDRALL
                     AAHGAVSEPVALAMADGIRALAGADIGLGVTGIAGPSGGSASKPAGTVVIAATTARDR
                     LVRTFRFPTGRARVRLFSVQLALDLARRLLTGQAPGGAFVYSPSAGRG"
     gene            complement(1336..1818)
                     /locus_tag="PLT35_09845"
     CDS             complement(1336..1818)
                     /locus_tag="PLT35_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOG29568.1"
                     /translation="MTTHPASTRAAVAVASFLYAGFAPFAPGTVGSAAAFLLIVPLRV
                     IGSPGLEWAAVVLLGLAGVWSARVAAEHLRVEDPGPVVIDEVVGMLVSLLWLPSTWPV
                     FLAAFLAFRLFDITKPWPARTLERLPGGWGIMADDVMAAIYANLAVQALVWWRPEWMR
                     "
     gene            complement(1854..2741)
                     /locus_tag="PLT35_09850"
     CDS             complement(1854..2741)
                     /locus_tag="PLT35_09850"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOG29569.1"
                     /translation="MKCPKCGYLGFESADRCRNCGFDFSLADAGGGRPGRGVPPLGAV
                     GGWTPQGEAGGARGTRHRRDGPLAGLGIDRPLLGQPEGTPVDLPLFGDADRIPLPPPP
                     RPPLVVRRSTPTPARLRPRPAQQADALPLDLEEPAEPIGSGPPAGPDGSRASATPAAA
                     PPVRRATAAAIDLGLLLLVDIVVLHFTLAVCGLTLNDVRVVPALPMGVFLAILNGGYL
                     VLFTGTLGQTLGKMAASIEVVTDRHGTVDLSRAGIRSAAMVLSLLTAGLGWLVAFVGD
                     GRSLHDRLAGTRVARVAGP"
     gene            complement(2738..3610)
                     /locus_tag="PLT35_09855"
     CDS             complement(2738..3610)
                     /locus_tag="PLT35_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29570.1"
                     /translation="MTEPRLADPHAPSDPLDLDREAKIDELLLAGLEHYFDGRFQEAI
                     NVWGRVLFLDRGHARARAYIDRARGALAERQRRTEELVEEGAAALDRGDGAAARRLLS
                     SAVANGDPHDLARPHLERLTRLASPTPPRGRRRAGTRAAAPGGPVARRGAGEPQRPVR
                     TLPILGAALAIAVLFLFVASRDWLGPLVAPGIRTSPGAEAAALAPPPLPVPRPAEIAL
                     ARARVLHASGYMKAALAALDGVSAADPLAPDAARLRAEIQEALIAPRAEPPPAVEEAE
                     PSETAAARPAGEPR"
     gene            complement(3633..>3875)
                     /locus_tag="PLT35_09860"
     CDS             complement(3633..>3875)
                     /locus_tag="PLT35_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG29571.1"
                     /translation="GAPHADPAGAPGEIRPLHDARDAFERDYILAALARQQGNISKTA
                     EALGVERSNLYRKMRALGIAPSRGGEREDEARESGV"
BASE COUNT          544 a         1436 c         1429 g          466 t
ORIGIN      
        1 gccaggaaca ggcgcatcag ccgcgaccgg cggagggcga gtacacgaaa gccccgccgg
       61 gcgcttgtcc ggtgagcagc cggcgcgcga gatcgagcgc gagctgcacc gagaacaggc
      121 ggacgcgcgc gcgccccgtc gggaagcgaa acgtgcgcac gagccggtcg cgcgccgtcg
      181 tcgccgcgat gacgaccgtg ccggcgggct tcgacgccga gcccccggaa ggccccgcga
      241 tgccggtgac gccaagcccg atgtcggcgc ccgccagcgc ccggatgccg tctgccatcg
      301 cgagggcgac gggctcactg acggcgccgt gcgccgcgag aagcgcccga tcgacgccaa
      361 gatgagccgc cttcgcctca ttgctgtagc agacgacgtt caacccgacg tagtccgagc
      421 tgcccgggac ctccgtcaga cgcgccgaca ccaggccgcc ggtacacgac tccgcgacgc
      481 cgatgcgcca gccgcgtgcc cgcagcaggc cgccgacggt ctcctcgatc gtgcgcccgt
      541 cggtgctgta cacgtcggcg cccaggatgg ccgtcagcgc gccgacggcc gcttccagcc
      601 tggcgctcgc ccgcgccgcc gacgccgagc gcagcgtcag atcgagttcg acctgtccgg
      661 gcgacgacag aatcgtcgtc tcgacgggcg ggtcctcgtg aagccactgc gagtagaccg
      721 gggccacggc ctcgtcggtc tcggattcag tccgcccgca gactctcagg acgcgccggt
      781 gcaggcgttc gtctccggcg aggctcgcga gccggccctc gaccaccgac tcgagcatcg
      841 gtgtcaactc ggccggcggg cccggcagga ggacgaggat cgagccttcg tgctcgatcc
      901 acaggcctgg cgccgtgccc gccgcgttcg gcagcacgac ggccccatcg ggcaccaggg
      961 cctgacgccg gttccgctcc ggcatcctgg cgccgcgcgc ctcgaagcgt cggcggattt
     1021 cgtccacgat gccggggtcc tcgctcattg cgcgcgacgt gacgtcggcc acgacgtctc
     1081 tggtcaggtc gtcgtcggtg gggccgaggc cgccgcagag gacgaccagc tccgttctgg
     1141 ccatcgcgcc gcgcaccgcg ctccgcaggt cgtcggcgcg gtcgccgacg atcgtctttg
     1201 cccggatctc gatgcccagg tcattgagcc gcctggtgat caccagcgag ttcgtgtcga
     1261 ggcgcggagg ggccagcaac tcggtgccga cggcgatgat ctccgcccac cgaaggcgcc
     1321 gggccgggga ggcgctcatc gcatccactc cggccgccac cacacgaggg cctgcacggc
     1381 gagattcgcg tagatggcgg ccatcacgtc gtcggccatg atgccccacc cgcccggcag
     1441 ccgctccagc gtgcgcgcgg gccagggctt ggtgatgtcg aagaggcgga acgcgaggaa
     1501 cgcagccagg aaaacgggcc aggtggaggg aagccagagg aggctcacca gcatgccgac
     1561 gacctcgtcg atgaccacgg gaccggggtc ttcgacgcgc aagtgctccg cggcgacccg
     1621 cgcgctccag acgccggcga ggcccaggag caccacggcg gcccattcga ggccgggcga
     1681 cccgatcacc cgcagcggca cgatcagaag gaacgcggcg gccgatccca ccgtccccgg
     1741 cgcgaacggc gcgaaccccg cgtagagaaa cgacgccacc gccacggcgg cgcgcgtgga
     1801 tgcggggtgt gtggtcatga cggggcgccc tgggcgagca ggcgggaggg cgctcacggc
     1861 ccggcaacgc gcgcgacgcg cgtacccgcg agccgatcgt gcaagctgcg cccgtcgccg
     1921 acgaaggcga cgagccagcc gaggcccgcc gtcagcagcg acagcaccat cgcggcgctc
     1981 cggatgccgg ccctcgacag atccaccgtg ccgtgccgat cggtcacgac ctcgatggag
     2041 gcggccattt tgccgagtgt ctgcccgagt gtcccggtga acagcacgag gtagccgccg
     2101 ttcaggatcg ccaggaacac gcccataggc agggccggca cgacgcgcac gtcgttcagc
     2161 gtcagcccgc agacggcgag ggtgaagtgc agcaccacga tgtccacgag gagcagcagg
     2221 ccgagatcga tggcggccgc ggtcgcgcgg cggacgggcg gcgccgccgc gggtgtggca
     2281 gaagcgcgag atccatccgg cccggccggc ggcccgctgc cgatgggctc cgccggctcc
     2341 tcgagatcga gggggagggc gtcagcctgc tgggcggggc gcggccggag tcgcgcaggc
     2401 gtcggcgtcg agcggcgaac cacgagcgga ggccgcgggg gcggaggcag cgggattcgg
     2461 tcggcgtcgc caaacagcgg caggtcgacc ggcgtgccct ccggctgccc taggagcggc
     2521 ctatcgatgc cgaggcccgc cagcggtccg tcccgccgat gtcgcgtccc acgcgcgccg
     2581 cctgcctcgc cctgcggcgt ccagccgccg acggcgccta gcggcggcac cccgcgtccg
     2641 gggcgccccc cgcccgcgtc ggcgagggag aagtcgaacc cgcagttcct gcagcggtcc
     2701 gcgctctcga atccgaggta cccgcacttc gggcacttca tctcggctct cccgccgggc
     2761 gggcggcggc cgtttccgat ggctctgcct cctcgacggc cggcggcggt tccgcacgcg
     2821 gcgcgatcaa cgcctcctgg atctcggcgc gcagtctggc ggcatccggc gcaagcgggt
     2881 cggctgccga cacgccgtcg agggcagcca gcgcggcctt catgtagccc gaggcgtgca
     2941 gaacgcgcgc gcgggccagg gcgatttcgg ccggccgcgg caccggaagc ggcggcggcg
     3001 cgagcgccgc ggcctcggcg cccggagacg tccggatgcc cggcgccacg agggggccga
     3061 gccagtcgcg cgacgccacg aacaggaaga ggacagcgat cgccagcgcg gcgccgagaa
     3121 tcggcagcgt gcgaaccggc ctctggggct ccccggcgcc ccggcgggca acggggccgc
     3181 cgggcgccgc tgcccgcgtt ccggcgcgcc tgcgcccgcg tggcggcgtc ggcgaggcca
     3241 gccgcgtcag gcgttcgaga tgggggcggg ccaggtcgtg gggatcgccg tttgcgacgg
     3301 ccgacgacag gagccgcctg gcggcggctc cgtctccgcg gtcgagggcc gcggcgcctt
     3361 cctcgaccag ttcctcggtc cttcgctgcc gctctgccag cgcccccctc gcccgatcga
     3421 tatacgcgcg cgcccgcgca tgcccccggt cgagaaagag cactcgcccc cagacgttga
     3481 tggcctcctg gaaccgcccg tcgaagtagt gctcgaggcc cgccagcagc agttcgtcga
     3541 tcttggcctc gcgatccaag tcgagggggt cggacggggc gtgtgggtcc gcgagtctcg
     3601 gctcggtcat tggggatacg ctcgcccgcc gattatacgc cagactcgcg ggcctcgtcc
     3661 tcgcgctcgc cgccgcgcga cggcgcgatg ccgagggccc gcatcttgcg gtacaggttg
     3721 ctccgctcga cgccgagcgc ctcggcggtc ttcgagatgt tcccctgctg gcgagccaag
     3781 gccgcgagga tgtagtcgcg ctcgaaggcg tcgcgcgcgt cgtggagcgg ccggatctcc
     3841 ccgggcgccc cggccggatc ggcgtgcggg gcccc
//
LOCUS       DAOSIF010000293         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20701_length_3872_cov_3.757925, whole genome shotgun sequence.
ACCESSION   DAOSIF010000293 DAOSIF010000000
VERSION     DAOSIF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20701_length_3872_cov_3.757925"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1176)
                     /locus_tag="PLT35_09865"
                     /pseudo
     CDS             complement(60..1176)
                     /locus_tag="PLT35_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(782..898)
                     /locus_tag="PLT35_09865"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(1148..1456)
                     /locus_tag="PLT35_09870"
     CDS             complement(1148..1456)
                     /locus_tag="PLT35_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29572.1"
                     /translation="MTFRTLLVIKALVCLVFGVILLAAPARLFGLLGEPVSGAGAFEA
                     RECGAAVTATLVPCWLARGIPWTSRRSHVLGRVNRTGIVGGPVRREAGAHGTTSEVLT
                     "
     gene            complement(1675..3066)
                     /locus_tag="PLT35_09875"
     CDS             complement(1675..3066)
                     /locus_tag="PLT35_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOG29573.1"
                     /translation="MRRRDFLKTGSAALAGAAVSSRAGAARGDAGPPAAAVRQTAPAA
                     LAPRFGDGRDWWFQKRFGLFVHWGLYAINGFHEQEQWRRRVPRREYVQLAAQWNPVAF
                     DPDEWLDLMRDAGMSYVCVTAKHHDGFCLWDTKLTPFNTMNTPFGRDILRMLADACHR
                     RGVPLCVYYSIADWDHPNYPNRGRHHELPPQAQDQPDFSRYLEFVRGQVRELCTDYGA
                     ISGFWWDMNVEQHADPSMNAMIRRLQPSAVINDRGFDPGDFGTPERDADAAGEDLGPF
                     SKRTEACQSVGVESWGYRRDESYYTDRHLLRSIDTYRARDANYLLNVGPKPDGTIPAV
                     PAGILRRIGRWCAAVGESFDGTHPASDLTANRSVLLTRKGHTLYVHLHRDPVSDSVKL
                     KPLARAPKRATLLNTGRPVAWALDMAPSDHAEQAAYLRLTGLPANEFANGVMVVRLDF
                     DGDPGGGARAGSG"
     gene            complement(3379..>3872)
                     /locus_tag="PLT35_09880"
     CDS             complement(3379..>3872)
                     /locus_tag="PLT35_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29574.1"
                     /translation="IGRSLTGIRAGDVVRAADYLKGRPDADAARLAGVARGEMGAVLL
                     HAAALDESIAQVALVDPLVSWASLVLNEYYAPKYMPHAVAGSIGRYDLPDLAAALAPR
                     RLLALGPVDEMGRPAAQASIDAGFAPVRAAYAAVPGASGALDIRAVKGLEELGSVLAG
                     WIR"
BASE COUNT          552 a         1407 c         1339 g          574 t
ORIGIN      
        1 cttttcaatt cctatgtgct ccgctcgcgc ggaatgtctg tcacatcagg gctaaatcag
       61 tccacccagg tcagcgtggc gaattcgaca gcctcctcgt ggcgccaggg tccccggcgg
      121 tggatgacct cggtcttgaa gagcccgatc accgtctccg cgagcgcatt gtcgtaggcc
      181 tcgccggtgc tgcccaccgg cggcgcgatg ccggcgtcca ccaagcggtc ggtgtagcgc
      241 atcgacagat actgggtgcc ccgatcgctg tgatgcacca agccggcgaa gccggcgccg
      301 cggcggtccc agagcgcttg ctccagggca gcccgcacga cgtccgtgcg caccgacgcc
      361 gagacccgcc agccgacaat gcgacgcgcg aacgcatcga tgacaaaggc gacgtagacg
      421 aatccggccc acgtcgcgac gtaggtgaaa tccgccaccc ccggctgatt ggggcgcgtc
      481 gccgtgacgt tccggtcgac gagatcggcc ggccgttccg cggcgggggg gcgtgggtcg
      541 tcgtcaccca tgcccgaccg cgcacggcgc cctggaggcc catcgcgcgc atcaggcgtc
      601 gcacggtaca gagcgcgacc gcggcccctt cccgcttggg ctgtctccac accttccggg
      661 gcccacacac tggtggtgtt catcccagac gcgccgaatc tccttgcacc acgcctcgtc
      721 tcgttgcgca cgcgccgacc gccgggccgg gtcctgctgg cgctcccgat gccagtagcg
      781 acggggcgat cgacaggagg ctgcagatcg gctcaacccc atacatctcc cggtgttcgt
      841 caatgaacgt cagcatcacg tcggtcggcg gtcgagctcc gcctgggcga cacacgccgc
      901 ggccttgcgg agaatctcgt tggcgcgctt gaggtcacga ttctcgcgct cgaggtccgt
      961 taggcgctgc cgttcgtccg tggtgagccc cgcgctctgg cccgcctcgc gctccggttg
     1021 ccggacccac ttccgcaggg tctcactgct gcagcccagt ttcggggcga tggcttccat
     1081 cgccgcccac ggcgacccgt gggcggccgc gtgctcggtt accatccgaa ccgcacgctc
     1141 ctgcatctca ggtgagtacc tcgctggtcg tcccatgggc tccagcctct ctccgaacgg
     1201 gccctccgac aatcccggta cgattcacgc gtcccagaac gtgagagcgt ctcgatgtcc
     1261 atgggatccc tctcgcgagc cagcagggca cgagggtggc cgtcaccgct gccccgcact
     1321 cgcgggcctc gaaggcccca gcgccgctga cgggctcacc cagcaggccg aacagccggg
     1381 caggtgcggc gaggaggatg accccgaaga ccaggcagac gagcgccttg atgacgagga
     1441 gcgttcggaa cgtcatcgtg agcctcccgt gtctcgcctg cgtccgcgcg tcgccggtct
     1501 tcggcacccg aatccggacc ccggcgcgca ggtggagcgc agcctgcggg acactccagt
     1561 tgtcgtgatg cgggtatgtt acgccgaagg ggaggcggcc tcaaatgccg ccagcggcgg
     1621 cccgcggcca ggggcccgca cgtgtccgcc ggccggcagc gccggccggc ggcttcagcc
     1681 ggagcccgcg cgcgcgccgc ccccggggtc gccgtcgaag tccagcctga cgaccatcac
     1741 gccgttggcg aactcgttgg cgggcaggcc ggtgaggcgc aggtaggccg cctgctcggc
     1801 gtggtcgctc ggcgccatgt cgagggccca cgccacgggc cggccggtgt tcagcagcgt
     1861 tgcccgcttc ggcgccctgg cgagaggctt cagcttcacc gagtcgctga cggggtcgcg
     1921 gtgcaggtgc acgtagaggg tgtggccctt gcgcgtcagg agcacgctgc ggttcgcggt
     1981 caggtccgat gcgggatgcg tcccgtcgaa cgactctccc acggccgcac accaccggcc
     2041 aatccggcgc aggatgcccg ccggcacggc cggaatcgtc ccgtccggct tcgggccgac
     2101 gttcagcagg tagttcgcgt cgcgcgcacg gtacgtgtcg atgctgcgca agaggtggcg
     2161 atcggtgtag tagctctcgt cacgcctgta gccccagctc tccacgccga cgctctggca
     2221 cgcctcggtc cgcttgctga aggggcccag gtcctcgccc gccgcgtcgg cgtcccgctc
     2281 cggcgtgccg aagtcgccgg gatcgaaccc gcggtcgttg atcacggcgg acggctgcag
     2341 ccgccggatc atcgcgttca tggagggatc cgcgtgctgc tccacgttca tgtcccacca
     2401 gaagccggag atcgctccgt agtccgtaca gagttcgcgg acctggcccc tgacgaattc
     2461 caggtaccgc gagaagtccg gctgatcctg cgcctggggc ggcagctcgt gatggcggcc
     2521 ccggttgggg tagttcgggt ggtcccagtc cgcgatcgag tagtagacgc agagcggcac
     2581 gccgcggcgg tggcaagcgt ccgccagcat ccgcaggatg tccctgccga acggcgtgtt
     2641 catcgtgttg aagggcgtga gcttcgtgtc ccagaggcag aacccgtcat ggtgtttcgc
     2701 cgtcacgcag acgtagctca tgccggcgtc gcgcatcagg tcgagccact catcggggtc
     2761 gaacgccaca gggttccact gcgcggccag ttgcacgtac tcccgtctgg gcacgcgccg
     2821 gcgccactgc tcctgctcgt ggaacccgtt gatggcgtag agcccccagt gaacgaacag
     2881 cccgaaacgc ttctggaacc accagtcgcg cccgtcgccg aagcgcgggg cgagggcggc
     2941 gggcgccgtc tgccgcaccg cggcggccgg cggcccggcg tcaccgcggg cggcgccggc
     3001 acgagacgac acggccgcgc ctgccagcgc ggcggaaccg gtcttgagga aatccctgcg
     3061 tctcacgcgc accccccagt tgttgacgcc ggccgttgcg cggcccgcac gccgcggtgc
     3121 ggcgctccgg acctgcggaa gtctacttcg cccggcccca gggccggtgc gggccgagtc
     3181 ctctccgccc gaggcagggt ctcgcgggcc ggcggcatga ctgtcgtgcg ttgactgtcg
     3241 cgcgaatccc gccggcctga acaggcgcgg gctcgaagcg cagactcgcg agccgcgcct
     3301 ccgggaccga atagggcaac gggcggcgga gagggatcgt ccggaagcga agcaggcggc
     3361 gcgcggaggg ccgctctcct acctgatcca ccccgccagc actgaaccca actcctccag
     3421 gcccttgacg gcccggatgt ccagggcgcc ggaagctccg gggacggccg catacgccgc
     3481 gcgcacgggc gcgaacccgg cgtcgatgga ggcttgcgcg gccgggcggc ccatctcgtc
     3541 caccggcccg agggcgagca gcctgcgcgg cgcgagcgcg gcggccagat cgggcaggtc
     3601 gtagcggccg atcgacccgg ccacggcgtg cggcatgtac ttcggcgcgt agtactcgtt
     3661 caggacgagc gacgcccacg agaccagcgg atccacgagc gccacctgcg cgatcgattc
     3721 gtccagcgcc gcggcgtgga gcagcacggc gcccatctcg ccgcgcgcga cgccggccag
     3781 gcgcgccgcg tccgcgtcgg gccttccctt caagtagtcc gcggcgcgca cgacatcgcc
     3841 ggcgcggatg ccggtcaggc tccgcccgat ct
//
LOCUS       DAOSIF010000294         3871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20709_length_3871_cov_3.011006, whole genome shotgun sequence.
ACCESSION   DAOSIF010000294 DAOSIF010000000
VERSION     DAOSIF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20709_length_3871_cov_3.011006"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2277)
                     /locus_tag="PLT35_09885"
     CDS             complement(<1..2277)
                     /locus_tag="PLT35_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOG29575.1"
                     /translation="MLKRRLPVLILFFSAVVCLAPVAHAQGTIAPPQFTAHGKTYTVG
                     DDYFLANYTQLRDYFAMLDRASDRFKVVEIGTTAEGRPMIMAIITSPENHARLERYKA
                     IAGTLARAEVDEAAARALAAEGKAVVWIDGGLHATECINTQGLFTFAHQMASRNDPET
                     LRILNDVITLLVPVNPDGMDLVSDWYMREPEEARRSLAGLPVLYEKYAGHDNNRDSYA
                     VNLPETEAVNRQLYIEWYPQIMYNQHQTGPAGTVLFVPPFRDPLNYNVDPLVPMGIDL
                     VSAAIHNRFVTEGKAGATMRSGAPYSTWYNGGTRTTTGFHNMIGILTEIIGSPTPGSV
                     GLVLDNQLPRQDLPMPIPPQAVWHQRQSIDYIMSVNRAILDAASKHREDFLFRIWRMG
                     RNSIERGSRDHWTIQPSWIDQAKAQAARDAGGGPAAGGRGSGRGGADPRHYGAMLTPE
                     RRDPRGYILPSDQPDFNTATKFVNALIKTGIVVHRATAAFTVSGRTYPAGSYAIKAAQ
                     AFRPHLRDMLEPQDHPNDFLYPGGPPRPPYDVAGYTLAFQMGVQVDRILEAFDGPFEA
                     LPLVPLKIGGGRVTPAAGRTAGYLLDHRQADAFTATTRLLAAKESVYWLRAPFSANGR
                     SYAPGTIYVPSKATTKAVVEKVAADLNVSVEATSVRPAGEALQLGPVRIGLWDQYGGS
                     MPSGWIRYIFEQTHPAPYELVFPPALDAGSLNARFDVLIFPPGAIPAAPGEAAGSGRG
                     GDFMAAMAGPGGRGAGAGQ"
     gene            2673..>3871
                     /locus_tag="PLT35_09890"
     CDS             2673..>3871
                     /locus_tag="PLT35_09890"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29576.1"
                     /translation="MQARAAGRTLLRLALTLAAMGTTVLMASSADSRLGLTSGAAPQA
                     PFQLIIAPLPIGGSVSGPGLACGSGGTACQTAPGGATAVTLTAAPAPGYAFAGWGGDC
                     AGTALETTVEVDGPRTCSAVFASTGAPADGPPYSMTVVPTAGGRVRAAGIHCGIGDAL
                     CSVAVNRASTLGMVAEPSPGYTFRYWTGDCAGTSDSLWMWLAGPRTCSAVFTRTGGPV
                     PVTYRLTISPIPTGGTVRWDGLTCGSGGTACQVTYAAPTSAMLSAVPDAGYTFTGWGG
                     ACSGTALTSEVQVDGVKTCSAAFAPTGGGPPVNGPPYSMTIALPTGGRVRAAGIDCGG
                     GALKCSLTLPGPATLGMEATPATGFVFGGWTGDCTGTAASLWVPLTGPRTCGAVFSPT
                     GSVPVYQ"
BASE COUNT          529 a         1305 c         1419 g          618 t
ORIGIN      
        1 ctgcccggcg cccgcgcccc ggccgcccgg gccggccatc gccgccatga agtcgccgcc
       61 tctgccgctg cccgccgcct cgcccggcgc ggcggggatc gcgccgggcg ggaagatcag
      121 cacgtcgaac ctggcgttca ggctgccggc atcgagcgcg ggcgggaaga cgagttcgta
      181 cggcgccggg tgcgtctgct cgaagatgta gcggatccac ccggacggca tcgacccgcc
      241 gtactggtcc cagagcccga ttcgcaccgg gccgagctgc agggcctcgc cggcaggccg
      301 cacggacgtg gcctcgacgc tcacgttcaa gtcggccgcg accttctcga ccacggcctt
      361 cgtcgtcgcc ttcgacggca cgtagatggt ccccggagcg tagcttctgc cgttcgcaga
      421 gaacggcgcg cgcagccagt agaccgactc cttcgccgcg agcagccggg tcgtcgcggt
      481 gaacgcgtcc gcctgccggt ggtcgagcag gtagcccgcc gtccttcccg cagccggcgt
      541 aacgcgcccg ccgccgatct tcagcggcac gagcggcagc gcctcgaacg gcccgtcgaa
      601 cgcctccagg atgcgatcga cctgtacgcc catctggaag gcgagcgtgt agccggcgac
      661 gtcgtacggc ggccgcgggg gtcctccggg gtacaggaag tcgtttggat ggtcctgcgg
      721 ctcgagcatg tcgcgcaggt gcggcctgaa cgcctgcgcg gccttgatcg catacgaccc
      781 ggccgggtac gtcctgcccg ataccgtgaa cgccgcggtg gcgcggtgga cgacgatgcc
      841 ggtcttgatg agcgcattga cgaacttcgt tgccgtgttg aagtccggct ggtccgacgg
      901 cagaatgtag ccgcgcgggt cccgccgctc aggcgtgagc atggcgccgt agtggcgcgg
      961 gtccgctccg cctcgtccgc tcccgcggcc gcccgctgcc gggcctccgc cggcgtcgcg
     1021 cgcggcctgc gccttcgcct ggtcgatcca gctcggctgg atcgtccagt ggtcgcggct
     1081 cccgcgctcg atcgagttcc gccccatccg ccagatgcgg aacaggaagt cctcgcggtg
     1141 cttcgacgcc gcgtcgagga tcgcgcggtt caccgacatg atgtagtcga tcgactggcg
     1201 ctggtgccac accgcctgcg gcggaatcgg catcggcagg tcctggcgcg gcagctggtt
     1261 gtccaggacc aggccgacgg agcccggcgt cgggctcccg atgatctccg tgaggatgcc
     1321 gatcatgttg tggaagccgg tcgtcgtccg cgtgccaccg ttgtaccagg tcgagtacgg
     1381 cgcccccgac ctcatcgtcg cgccggcttt gccctcggtg acgaaccggt tgtggatggc
     1441 cgccgacacg agatcgatgc ccatcggcac gagcggatcg acgttgtagt tgaggggatc
     1501 gcggaagggc ggcacgaaca gcaccgtgcc ggccggaccg gtctggtgct ggttgtacat
     1561 gatctgcggg taccactcga tgtagagctg gcggttcacc gcctcggttt cgggcaggtt
     1621 gacggcgtac gagtcgcggt tgttgtcgtg gccggcgtac ttctcgtaca ggaccggaag
     1681 gcccgccagc gagcggcggg cctcctcggg ctcgcgcatg taccagtcgg acacgaggtc
     1741 catcccgtcc gggttgacag gcacgaggag cgtgatgacg tcgttcagga tgcgcagcgt
     1801 ctcggggtcg tttcggctcg ccatctggtg cgcgaaggtg aagagcccct gggtgttgat
     1861 gcactcggtc gcgtggaggc cgccgtcgat ccagacgacc gcctttccct cggccgccag
     1921 cgcgcgggcc gccgcttcgt ccacctcggc gcgcgcgagc gtcccggcga tcgccttgta
     1981 gcgctcgagg cgggcgtggt tctcggggga cgtgatgatc gccatgatca tcggccgccc
     2041 ctccgccgtc gtcccgatct cgacgacctt gaagcggtcg gatgcccggt cgagcatggc
     2101 gaaatagtcc cggagttggg tgtaattcgc caggaagtaa tcgtcgccca ccgtgtacgt
     2161 cttgccgtgg gcggtgaact gcgggggcgc gatcgttccc tgcgcgtgcg ccacgggcgc
     2221 aaggcagacc acggcggaga agaagaggat cagaacaggc agtcgccgtt tcagcatcga
     2281 agagtccttt cgggagcgaa ccctcgcgca atcgcgcgcg cgtacgatag cgcgacttgg
     2341 gtcgaaacac cacttcgaga cggcgccggc cggcgtgatg ggcccgggga ctgacagtct
     2401 gcaaggacgg gcgatcacaa gaacaaagtc tccctggggg cagagatctg gcgatggcgg
     2461 gcgggtggtc cgctgagatc gccgcgtatt ccgccacgat ccgcgcgcct tcgctctgag
     2521 gcgtgctcgc gaagagaggc acgggccttg cagcacccac atatcaaagg ccaaggccgt
     2581 ctgctcgctc cctgagatct ccgcctgttg ggggtctgtc caggtctcgg acccggacag
     2641 cgcctggtag taccggctct gaggatggac tcatgcaggc tcgagcggcc ggacgcacgc
     2701 tgctgcggct ggcgctgacg ctcgcggcta tggggaccac ggtcctcatg gcgtcctctg
     2761 ccgattcgcg gctggggttg acgagcgggg ctgcgcctca ggctcccttt cagctcatca
     2821 tcgcgcctct gccgataggc ggctccgtgt cgggacccgg gctggcctgt ggttcgggcg
     2881 gcacggcctg ccagacagcc cccggcggcg caacggccgt cacgctcacc gccgcgccgg
     2941 cgccggggta cgcgttcgcg ggctggggag gcgactgcgc gggaacggcc ctcgagacaa
     3001 cggtcgaagt cgacgggcct cggacgtgca gcgcggtgtt cgcgtccacg ggtgcgccgg
     3061 ccgacgggcc cccgtattcg atgacggtgg tgccgacggc gggcgggcgc gtccgggcgg
     3121 cgggcatcca ctgcgggatt ggcgacgcgc tgtgcagcgt ggcggtgaac cgggcgagca
     3181 cgctcgggat ggtggcggag ccgagtcccg gctacacgtt ccgttactgg accggagatt
     3241 gcgcgggcac gagcgacagc ctgtggatgt ggctggccgg ccctcgcacc tgcagcgcgg
     3301 tgttcacgcg gacgggcggc ccggttccgg tgacgtatcg gctcacgatc agcccgatcc
     3361 cgacgggcgg caccgtgcgc tgggatggac tcacgtgcgg ctcgggcggc accgcctgcc
     3421 aggtgacgta tgcggccccc acgtcggcga tgctgtcggc ggttcccgac gcgggctaca
     3481 cgttcacggg atggggcggc gcctgcagcg gcacggcgct gaccagcgag gtgcaggtcg
     3541 atggcgtcaa gacctgttcc gccgccttcg cgccgacggg cggcgggccg ccggtgaacg
     3601 gccctccgta ctcgatgacc atcgcgctgc cgacgggcgg cagggtccgg gcggcaggca
     3661 tcgactgcgg cggcggagcg ctgaagtgca gcctcacgct gccggggccc gcgacgctcg
     3721 gcatggaggc gacgccggca accggcttcg tgttcggcgg atggacgggg gactgcacgg
     3781 gaacggccgc gagtctctgg gtgccgctga ccggtccccg gacgtgcggc gcggtcttct
     3841 cgccgacagg ctcggttccc gtctaccagt t
//
LOCUS       DAOSIF010000295         3865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20744_length_3865_cov_3.839108, whole genome shotgun sequence.
ACCESSION   DAOSIF010000295 DAOSIF010000000
VERSION     DAOSIF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20744_length_3865_cov_3.839108"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PLT35_09895"
     CDS             <1..1432
                     /locus_tag="PLT35_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HOG29577.1"
                     /translation="RAFHQRIDAALADRALGDALALTTGRLAGGRAAAMAAFADAEAA
                     RDEARRIRAAAIARLDQLLERFEREATARGCRVHWAETAADATRIVTAIAQEARVTLA
                     VKSKSMVSEEIELNAALERAGVRVVETDLGEFVVQIAGDRPSHIITPIIHRSRHDVAR
                     LFREKANAAPEDVADIPAMTAFARRTLRAEFLQAGMGISGANVAVAETGTVCLVTNEG
                     NGRLTTTLPRVHVALVGIERIVPSAADLAAVLSLLARSATGQALSVYTSLISGPRRAG
                     AGPGPAKGGEPDGPDAVHVVFVDNGRSRLLGGPLAEILYCIRCGACLNVCPVYQKVGG
                     HAYGGVYPGPVGSVVMPGLLGLEAWSELAQASSLCGACREACPVRIDLPRLLLEVRRQ
                     AAAKRLAPRWLRVAMPVYAAVARRPRLYRSAVAAAGLLGRMAAKGGWIRRLPGPLGAW
                     TASRDFPAPATRSFLQQHRLRKEAAR"
     gene            1429..2109
                     /locus_tag="PLT35_09900"
     CDS             1429..2109
                     /locus_tag="PLT35_09900"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOG29578.1"
                     /translation="MTGARGRILESIRSGLVRAALPPAEHAGVPDAKAGGGGSIEAMV
                     ERFGAVLTALSGRMHRASSPEVPALVADIAAAHGARSFLAWDETELGCPGLLEGLAAR
                     GLARVDYRMPTDPDARFRLAQALEPIGLGVTGGHAAIAENGAVVLVSGPGRGRLVSLL
                     PAVHVAIVSERRLRPTLADLVREEPRLLDAGSNVVLVAGPSRTADIEMALSHGVHGPK
                     HVHVILTA"
     gene            2106..3095
                     /locus_tag="PLT35_09905"
     CDS             2106..3095
                     /locus_tag="PLT35_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOG29579.1"
                     /translation="MTHTLLPGLGAIDLDRFVRLGACSDGRFAEGRDGVRLVLAPADR
                     RVEFIVFEDRTLAYVRSALGYPAYYPVHPVALERPVRAVLMDLDGTTVRSEPFWVLMI
                     ERATGSLLGDPSFRLDESDLPFVSGFSVSEHLEHCIRKYCPDRSVEDARRCYFEHTHR
                     EMREILEGRGRQDAFVPAPGLKPFLLALKREGIRIGVVTSGLHEKAWPELVAAFRAMD
                     LGDPRAFYDAIVTAGFPVRPGEAGTLGELSPKPHPWLYAEAARVGLGIPFDQRHRVVG
                     IEDSGAGVCAIRLAGFTAFGMAGGTIEASGARPLCQHYCASFDEVLAILHGSV"
     gene            3239..>3865
                     /locus_tag="PLT35_09910"
     CDS             3239..>3865
                     /locus_tag="PLT35_09910"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOG29580.1"
                     /translation="MNLILLGAPGAGKGTQAEAITRALGIPQISTGDLIRAAIREQTP
                     LGREFQSYANAGALVPDGLVNRIVEDRLSQPDCNGGFLLDGFPRTLAQAEWLTGALAA
                     AGRRIDRVLLVEVDDAVILERITGRRSDPETGRVYHVTFDPPPADVAARLVQRPDDTE
                     AVLTKRLREYHEKTNQVVPYYERLGLLSRIDGLGSVPEVRQRVLGALGV"
BASE COUNT          496 a         1413 c         1399 g          557 t
ORIGIN      
        1 ccgcgccttc caccagcgga tcgacgcggc cctggccgat cgcgcgctgg gcgacgccct
       61 ggcgctcacg acgggccgcc tggccggggg ccgcgccgcc gcgatggccg ctttcgccga
      121 cgccgaggcc gccagagacg aggcgcgccg gatccgcgcc gccgccatcg cccgcctgga
      181 tcagctcctc gagcggttcg aacgcgaggc gaccgcgcgc ggctgccgcg tccactgggc
      241 cgaaacggcc gccgatgcca cccggatcgt gacggcaatc gcgcaagagg cgcgcgtcac
      301 gctcgcggtg aagtcgaagt cgatggtctc cgaggagatc gagctgaacg ccgccctcga
      361 gcgggctggc gtccgggtcg tcgaaacgga cctcggcgag ttcgtcgtgc agatcgccgg
      421 ggaccgcccc tcccacatca tcacgcccat cattcaccgg tcgcgccacg acgtggcgcg
      481 gctcttccgc gaaaaggcaa acgccgcgcc ggaggacgtc gccgacatcc cggccatgac
      541 cgccttcgcc aggcgcaccc tgcgcgcgga gttcctgcag gcgggcatgg gcatcagcgg
      601 cgccaacgtg gcagtcgccg aaaccggcac cgtgtgcctc gtgaccaacg agggcaacgg
      661 gcgtttgacg acgacgctgc cgcgcgtgca cgtggcgctg gtcggcatcg agcgcatcgt
      721 gccctcggcc gcggacctcg ccgccgtcct gtcccttctc gcgcgcagcg cgaccggcca
      781 ggcgctgtcg gtctacacca gtctcatctc cggcccgcgc agggcaggcg ccggaccggg
      841 ccctgcgaag ggcggcgagc cggacggccc ggacgctgtg cacgtcgtgt tcgtggacaa
      901 cggcaggagc cgcctgctgg gagggccgct ggccgagatc ctctactgca tccggtgcgg
      961 cgcctgcctc aacgtctgcc ccgtttacca gaaggtgggc gggcacgctt acggcggcgt
     1021 ctatccgggg cctgtcggat ccgtggtcat gcccggcctg ctggggctcg aggcatggtc
     1081 ggaactggcg caggccagca gcctctgcgg cgcctgccgc gaggcgtgcc cggtgcggat
     1141 cgacctgccg cgcctcttgc tggaggtgcg ccggcaggca gcggcgaagc ggctggcgcc
     1201 gcggtggctc cgggtcgcca tgccggtcta tgccgccgtc gcgaggcggc cgcggctcta
     1261 tcggagcgcc gtcgccgccg ccgggctgct cgggaggatg gccgccaagg gcggctggat
     1321 ccggcggctg cccggcccgc tcggcgcctg gaccgcgtca cgggattttc ccgcccccgc
     1381 aacccggtcg ttcctgcagc agcaccgcct gcggaaggag gcggcgcgat gaccggagcc
     1441 cggggccgaa tcctggagtc catccgcagc gggctcgtgc gcgcggcgct cccgccagca
     1501 gagcatgcgg gtgttcccga cgcgaaggcc ggcggcggcg gatccatcga ggcgatggtc
     1561 gagcgcttcg gcgccgtcct gacggcgctc tcgggccgca tgcatcgggc gtcgtcaccg
     1621 gaagtgccgg ccctcgtcgc cgacatcgcg gccgcgcatg gggcccggtc gttcctggcg
     1681 tgggacgaga cggagctggg ctgccccggg ttgctggaag gactggcggc gcgcggcctg
     1741 gcccgcgtgg actaccggat gccgacggac cctgacgccc ggttccgtct cgcgcaggct
     1801 ctggagccga tcggcctggg cgtgaccggc ggccacgccg cgatcgccga gaacggggcc
     1861 gtggttctcg tcagcggacc cggccgcggg cggctcgtgt cgctgctccc ggcggtccac
     1921 gtcgccatcg tgtcggagcg gcggctgcgg cccaccctgg cggacctggt ccgtgaagag
     1981 ccccgcctgc tggacgcggg gagcaacgtc gtgctggtgg cggggcccag ccgcacggcg
     2041 gacatcgaga tggcgctgtc gcacggcgtc cacggcccga agcacgtcca cgtgatcctg
     2101 acggcatgac acacaccctg ctgcccggcc tcggcgcgat cgacctcgat cgcttcgtgc
     2161 gcctcggcgc gtgcagcgac ggccgcttcg ccgaaggccg cgacggcgtg cggctcgtgc
     2221 tggcgcccgc cgaccgccgc gtcgagttca tcgtgttcga ggatcggacg ctggcctacg
     2281 tgcgctccgc cctcggctac ccggcgtact accccgtgca tcccgtggcg ctcgaacgcc
     2341 ccgtccgggc cgtcctcatg gacctggacg ggaccaccgt gcggagcgag ccgttctggg
     2401 tgctgatgat cgagcgggcg acggggagcc tcctcggcga cccgtcgttc cgcctcgacg
     2461 agtcggacct gccgtttgtg tcggggttca gcgtgtcgga gcacctggag cactgcattc
     2521 gcaagtactg tccggaccgt tccgtggagg acgcgcgccg gtgctacttc gagcacacgc
     2581 accgcgagat gcgcgagatt ctcgagggac ggggccgcca ggacgcgttc gtcccggccc
     2641 ctggcctcaa gcccttcctg ctggccttga agcgcgaagg catccggata ggcgtcgtga
     2701 cgtcgggctt gcacgagaag gcgtggccgg aactggtggc cgcgttccgg gccatggatc
     2761 tcggcgatcc gcgcgccttc tacgacgcga ttgtgacggc cggcttccct gtccggcccg
     2821 gcgaggcggg cacgctgggc gaactgtcgc ccaagcctca tccatggctc tacgcggagg
     2881 ccgcccgcgt cgggctgggg attcccttcg accagcgcca ccgcgtcgtg gggatcgagg
     2941 acagcggcgc cggcgtgtgc gccatcaggc tcgccgggtt cacggcattc ggcatggccg
     3001 gcggcaccat cgaggcaagc ggcgcgcggc cgctctgcca gcattactgc gcgtccttcg
     3061 acgaggtgct cgcgatcctc cacggctccg tctgaacctc ggcgtctgga tgccgcgccc
     3121 cgagtcccga gccccgagtc ctctgccctg cccttgtctc cctcgatccc atgcactagg
     3181 atggccgggc ccctccgcat ggccggcgcg cgccggcctt cacgacacga ggaccttcat
     3241 gaatctgatt ctccttggcg ccccgggcgc gggcaaaggc acgcaggccg aggcgatcac
     3301 gcgggcgctg ggcattccgc agatctccac cggcgacctc atcagggcgg ccatccgcga
     3361 gcagacgccg ctcgggcggg agttccagtc gtacgccaac gctggggcgc tcgtgcccga
     3421 cggcctcgtg aaccggatcg tcgaggacag gctctctcag cccgactgca acggcggctt
     3481 cctgctcgac ggcttccccc gcaccctcgc ccaggccgaa tggctgaccg gcgcgctcgc
     3541 cgcggcgggc cggcggatcg atcgggtgct gctcgtcgag gtggacgacg ccgtgatcct
     3601 cgagcggatc acggggcggc gcagcgatcc cgagacgggc cgggtctacc atgtgacgtt
     3661 cgatccgccg ccggcggacg tggcggcgcg gctcgtgcag aggccggacg acaccgaggc
     3721 cgtgctcacg aagcgcctgc gggaatatca cgaaaagacg aaccaggtgg tgccctacta
     3781 cgagcgcctc ggcctcctgt ccaggatcga cggcctgggc agcgttcccg aggtcaggca
     3841 gcgggtcctc ggcgcgctcg gcgtc
//
LOCUS       DAOSIF010000296         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20785_length_3858_cov_3.324744, whole genome shotgun sequence.
ACCESSION   DAOSIF010000296 DAOSIF010000000
VERSION     DAOSIF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20785_length_3858_cov_3.324744"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLT35_09915"
     CDS             <1..398
                     /locus_tag="PLT35_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOG29581.1"
                     /translation="VKETDMRHAISTNDAPKAIGPYSQAVRAGSLVFVSGQGHVDPAT
                     GEVVAAGDIAAATRRVMDNIGAILQAAGASFDHVVRTTVYLADMNDFAAMNAAYAEYF
                     SSPAPARTTIQAARLPKDLRVEIDVIASL"
     gene            complement(382..2589)
                     /locus_tag="PLT35_09920"
     CDS             complement(382..2589)
                     /locus_tag="PLT35_09920"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOG29582.1"
                     /translation="MIRFEDLVARAQTYMPEADIELLRRAYVFSAMAHQGQVRQSGQP
                     YLVHPLEVAHLLADMKLDPAAIIAGLLHDVVEDTLTTVERIEELFGREVAHLVEGVTK
                     ISVIPFSSSEERQAENFRKILLAMVDDIRVVLVKLADRLHNMRTLGAMPEERRQRTAR
                     ETLDIYAPIANRLGMSRLKNELEDLAFRHLEPEAYEALKARVERRRKATEGLLDEMRR
                     SMAATLKEAQIPVIEIDGRSKRLYSIHQKLRRQQIDLEQVYDFLAIRVITDTIKDCYA
                     ALGIVHHSWPPVPGRIKDFIATPRPNGYQSLHTSVMTTKGVAVEVQIRTQEMHRLAEE
                     GIASHWKYKEGRVGAANDERYFQWLRQTLEWQQEVRDPQEFIQNLKVDLYPDEVYTFT
                     PKGQVKSLPRDSTVIDFAYSIHSDVGHQCVGARINGKMMPLRTRLRNGDVVEIVTSAK
                     HRPSRDWLNFVVTTRARNRIRAFLHAEEKARAIEIGRKLLEKAGRRFAVNLKALLEGP
                     GLDPIAPEWGVPNGLDLLAAVGYGRVGARQVLARASPDGELKEKRESAVRSAVKKVIG
                     LGSGDSIRVSGADDVLVVRARCCNPIHGERIVGYITRGKGVSVHAASCPNVTSLLYDP
                     ERRIDVEWTKSAQPGQYTVRLGIQVQDRKGMVAAVSAAIASVNTNIVDIEARTDDEQR
                     GWIRVTIEISDMKHLEKVMKSLRGIPGVVAVERARAVPLDLSRAPQGPATGTR"
     gene            2901..3848
                     /locus_tag="PLT35_09925"
     CDS             2901..3848
                     /locus_tag="PLT35_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29583.1"
                     /translation="MLRGLRRVAPLAAVLALAVLATGCGQYAKLQARQAFKEANLLYS
                     QQDYKRAAAKYEEVIQKDPSMSVAYFYLANSYDNLYKPSRKGEAENDSYLTKAVEYYQ
                     LAGEKEADPTLRKRSFEFLVAAYGADKLNDPSQQEPIVNRMIELDPQDVTNYFALAKI
                     REDAGDYEKAEEVLLQAKQARPNDPQVYLQLAGFYNRQEEFEQTIDALNQRAAIEPNN
                     PEAFYTISTYYWDKAYRDFRLKDAEKLDFVMKGIAEVDKALQLKADYMEALTYKNLLL
                     RLQANLEKDPSKQSALLKEADRLRDIALDMKKKQVAGIK"
BASE COUNT          620 a         1302 c         1283 g          653 t
ORIGIN      
        1 gcgtgaagga gacggacatg cgccacgcga tcagcacgaa cgacgcgccg aaggccattg
       61 ggccctactc gcaggcggtc cgcgcgggca gcctggtgtt cgtgtccggc cagggccacg
      121 tggacccggc gaccggcgag gtcgtggcgg ccggcgacat cgccgccgcg acacgccgcg
      181 tgatggacaa catcggcgcg atcctccagg ctgcgggcgc gtcgttcgac cacgtggtcc
      241 gcacgacggt ctacctggcc gacatgaacg acttcgcggc catgaacgcg gcctacgccg
      301 agtacttctc ctcgccggct ccggcgcgca cgaccatcca ggccgcccgc ctgccgaagg
      361 acctgcgcgt cgagatcgac gtcatcgcgt ccctgtagcc gggccctgag gggcccgcga
      421 caggtcgagc ggcacggccc tcgcccgttc cacggccacc actccgggga tgccgcgcag
      481 cgacttcatc accttctcca ggtgcttcat gtcgctgatc tcgatggtca cccggatcca
      541 cccgcgctgc tcgtcgtcgg tgcgggcttc gatgtccacg atgttcgtgt tgaccgacgc
      601 gatcgccgcg ctgaccgccg ccaccatgcc cttgcgatcc tgcacctgga tgccgaggcg
      661 caccgtgtac tgcccgggtt gggccgactt ggtccactcg acgtcgatgc gccgctcggg
      721 atcgtagagc aggctggtca cgttcgggca gctcgccgcg tgcacggaca cgcccttgcc
      781 gcgtgtgatg tagccgacga tccgctcgcc gtggatggga ttgcagcagc gggcgcgcac
      841 caccagcacg tcgtcggcgc cgctgaccct gatggagtcc ccgctgccga ggccgatgac
      901 cttcttgacg gcggacctga ccgcggactc ccgcttctcc ttcagctcgc cgtcgggcga
      961 cgcccgggcg agcacctggc gggcgcccac gcgcccgtag ccgacggcgg cgaggagatc
     1021 gaggccgttg ggcacgcccc actccggcgc gatcgggtcg agccccgggc cttccagcag
     1081 cgccttcagg ttgacggcga agcgccggcc agccttctcg aggagcttgc ggccgatctc
     1141 gatggcccgc gctttttcct cggcgtgcag gaaagccctg atccggttcc gggcccgcgt
     1201 cgtgacgacg aagttcagcc agtcccggct cggccggtgc ttcgcgctcg tgacgatctc
     1261 gacgacgtcc ccgttccgca gccgtgtccg caggggcatc atcttgccgt tgatgcgggc
     1321 gccgacgcac tggtgcccga cgtccgagtg gatcgaatag gcgaagtcga tgacggtgga
     1381 atcgcgcggc agcgacttca cctggccctt cggcgtgaag gtgtagacct cgtccgggta
     1441 gagatccacc ttcaggttct ggatgaactc ctgcgggtcg cggacctcct gctgccactc
     1501 gagggtctgc cgcagccact ggaagtagcg ctcgtcgttg gcggcgccga cgcgcccttc
     1561 cttgtacttc cagtgcgagg cgatgccttc ctccgccagc ctgtgcatct cctgcgtgcg
     1621 gatttgcacc tccacggcca cgcccttcgt cgtcatgacc gacgtgtgca gggactggta
     1681 gccgttgggc cgcggcgtgg cgatgaagtc cttgatgcgc ccgggcacgg gcggccagga
     1741 atggtggacg atgccgagcg cggcgtagca gtccttgatg gtatcggtga tgacgcggat
     1801 ggcgaggaag tcgtagacct gctcgagatc gatctgctgc cgcctgagct tctggtggat
     1861 gctgtagagc cgcttgctgc ggccgtcgat ctcgatgacg gggatctggg cctccttcag
     1921 cgtggcggcc atgctgcggc gcatctcgtc gagcaggccc tcggtggcct tgcgccggcg
     1981 ctcgacgcgg gccttcagcg cctcgtacgc ctcgggctcc aggtggcgga acgcgaggtc
     2041 ctcgagctcg ttcttgagcc ggctcatccc gagccggttc gcgatggggg cgtagatgtc
     2101 gagcgtctcc cgggccgtcc gctgccggcg ctcctcgggc attgccccga gcgtccgcat
     2161 gttgtgcagg cggtccgcca gcttcacgag gacgacgcgg atgtcgtcga ccatcgccag
     2221 gaggatcttc cggaagttct ccgcctggcg ctcctcgctg gacgagaagg gaatgacgct
     2281 gatcttcgtc acgccctcga ccaggtgcgc gacctcccgc ccgaagagct cctcgatccg
     2341 ttcgaccgtg gtcagcgtgt cttcgacgac gtcgtggagg aggcccgcga tgatggccgc
     2401 cgggtcgagc ttcatgtcgg ccagcaggtg ggccacctcg agcggatgga cgaggtacgg
     2461 ctgtccggac tggcgcactt gcccctggtg agccatcgcc gagaagacgt acgcccgcct
     2521 cagcagctcg atgtccgcct cgggcatgta cgtctgagcc cgggccacga ggtcttcgaa
     2581 gcggatcatg gcggccggtg ccgacgcgcc cgacgcacgc gcggccaggc ttcatcatag
     2641 gaagcgccct ccgggcggtc aagaagggac gccgccgcgg aggtcctcgt ccgacagaac
     2701 ggccctgcgc gccccgccgt gttgcttgac tcgccctttt ttggttcact atactggcgc
     2761 ggcttgggaa cctgtccctt cagtccggag gatcgccagt cccgccggcc tgaagggtgc
     2821 tgtgccgttt cttgtgggtc tgactgggcc gtctcaggcc gcttgcatcc gcgcgtcgcg
     2881 ggaaaggtaa gggggacatc atgttgaggg gacttcggcg ggtcgcaccg ctggccgccg
     2941 tgttggcgct cgccgtgttg gcgactggct gcggccagta cgcgaagctc caggccagac
     3001 aggcattcaa ggaagccaac ctcctgtact cgcagcagga ctacaagaga gcggcggcga
     3061 agtacgaaga ggtgattcag aaagacccct cgatgtcggt cgcctatttc tacctggcca
     3121 acagctacga caacctctac aagccgtcgc gcaagggcga ggcggagaac gactcgtacc
     3181 tgacgaaggc cgttgagtac taccagctcg ccggcgagaa ggaggccgat ccgacgctga
     3241 ggaaacggtc cttcgagttc ctcgtcgccg cctacggcgc cgacaagctg aacgatccct
     3301 cgcagcagga gcccatcgtc aaccgcatga tcgaactcga tccgcaggac gtgacgaact
     3361 acttcgcgct ggccaagatc cgcgaggacg cgggcgacta cgagaaggcc gaagaggtgc
     3421 tgctgcaggc gaagcaggcc cgcccgaacg atccccaggt gtacctgcag ctggcgggct
     3481 tctacaaccg gcaggaggag ttcgagcaga cgatcgacgc cctgaaccag cgcgcggcga
     3541 tcgagccgaa caatcccgag gcgttctaca ccatttcgac ctactactgg gacaaggcct
     3601 atcgcgactt ccgcctcaag gacgccgaaa agctcgattt cgtgatgaag ggcatcgccg
     3661 aggtcgacaa agccctccag ctgaaggccg actacatgga ggcgctgacc tacaagaacc
     3721 tcctgctccg gctccaggcg aacctcgaga aagacccgtc caagcagtcg gcgcttctca
     3781 aggaggccga ccgccttcgt gacatcgcgc tcgacatgaa gaagaagcag gtcgcgggca
     3841 tcaagtaggc cgagcgcc
//
LOCUS       DAOSIF010000297         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20943_length_3834_cov_4.872188, whole genome shotgun sequence.
ACCESSION   DAOSIF010000297 DAOSIF010000000
VERSION     DAOSIF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20943_length_3834_cov_4.872188"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..590
                     /locus_tag="PLT35_09930"
                     /pseudo
     CDS             93..590
                     /locus_tag="PLT35_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
     gene            587..1147
                     /gene="ruvC"
                     /locus_tag="PLT35_09935"
     CDS             587..1147
                     /gene="ruvC"
                     /locus_tag="PLT35_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG29584.1"
                     /translation="MRVFGVDPGSRRTGYGCIETDGTRHRLVACGAVTPPPGASFPER
                     LMAIHRELGRLLRRHRPDCVAVESVFHAANVRSALTLGQARGVALLAAVEAGYPLVEY
                     APAEVKLAVVGYGRAEKRQVGEMVRLLLGLVEAPAPNDASDALAVAICHVHSCGGAAL
                     APAAREAAPAAGRRRRAPTSWREYRP"
     gene            1144..1749
                     /gene="ruvA"
                     /locus_tag="PLT35_09940"
     CDS             1144..1749
                     /gene="ruvA"
                     /locus_tag="PLT35_09940"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOG29585.1"
                     /translation="MIAFLRGRLAGKQPNRIIVDVAGVGFDVAVPLSTFYKLQDEGSD
                     VALWIHTHVREDAIALYGFSTGLEQQIFERLIGIGGIGPKLALAVLSGIDVADLVRAV
                     RAGDVARLTAIPGIGRKTAERIGLELKDKLPDTFAADLAADESGAGADGGLRGDLLSA
                     LLNLGYHRPLAERAVDAALARAASPTFEAALKQALRELARR"
     gene            1753..2778
                     /gene="ruvB"
                     /locus_tag="PLT35_09945"
     CDS             1753..2778
                     /gene="ruvB"
                     /locus_tag="PLT35_09945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG29586.1"
                     /translation="MTDDSTRVTSMRADDEDAQFEAGLRPRTLDEFTGQDRVRENLHV
                     AITAAKQRREALDHVLLYGPPGLGKTTLAYVIGNELGVPVRSTSGPVIERPGDLAAML
                     TNLKEREVLFIDEIHRMAATIEEILYPALEDYELDIVIGQGPAARSVKVPLQPFTLIG
                     STTRAGLLTGPLRGRFGIVHRLDFYADADLDVIIRRSARILGAPISGDAAAEIARRSR
                     GTPRIANRLLRRVRDYAQVRAQGEITAPVTQAALAMMDVDEHGFDEIDRRLLRTIIDK
                     FGGGPVGVRTIAAAISEEQDAIEDIYEPFLLQIGFIDRTPRGRVATPRAYAYFGLQAP
                     GKDSRLW"
     gene            2772..3779
                     /locus_tag="PLT35_09950"
     CDS             2772..3779
                     /locus_tag="PLT35_09950"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOG29587.1"
                     /translation="MVTPNTIRQTRIGALATYVPPKTLDNHQLSQMVDTSDEWILQRT
                     GIKTRHIVEPGVAASDLGKEAALKTLAQAGLTPDDIDVIVVGTTTPDMFFPNTASLIQ
                     TKIGATKCWGFDVAAACSGFTYSVGIASDLVAAGRCNRALVVGTDVMSSITDYTDRTT
                     CVLFGDGAGAVIVEPAVEEGLGIIGCEYENDGTLWPALQMPGGGSLHPASHETVDKKM
                     HFMKQDGQTVFKFAVRKTEEITRKVLEKHGYTPDDVSLFVSHQANRRIITSAGERLGL
                     PESKVMMNIQDYGNTTAATIPLALDDAIRQGRLKKGDLVVLVSVGAGFTVGSVLLRWA
                     F"
BASE COUNT          646 a         1250 c         1404 g          534 t
ORIGIN      
        1 gccggtgatc gttttatcca acttgggaga agacgagaac atcaagcggg ccatccggcg
       61 ggggacgggg gaagagcccg gcgtgcagta cgaagaggac ctacgagggc tacgggccgg
      121 gcggcgtcgc gctgatcatc gagacgatga ccgacaacaa gaaccggacg gtcggcgaga
      181 tccggcacct gctcaccaag tacggcggcg acctcggcca gtcgaactcg gtgtcctgga
      241 tgttcgagaa gaagggctac atcgtcattg agaaggcgaa ggccaccgag gacgcgctga
      301 tgacggcggc gctggacgcc ggggcggacg atctgcgcga cgacggtgac aactgggaga
      361 tcgtgagcgc ccctgacatc ttcccggcgg tgcgggaggc catcgagaag ctcggcgtcg
      421 aaaccgcgtc ggcccaggtg gcgatgctgc cgaagaacca tgtgtcgatc caggggaagt
      481 cggcgcagag catgctgaag ctgctcgatc tcctcgagga tcacgacgac gtgcagcacg
      541 tgtggtcgaa catggacgtc gacgagaagg agctcgaggc attcgagtga gggtgttcgg
      601 cgtcgatccc ggatcgcggc gaacggggta cggctgcatc gaaaccgacg ggacccggca
      661 ccgcctcgtg gcgtgcgggg cggtgacacc gccgccgggc gcgtcctttc ccgagcgatt
      721 gatggccatc caccgggagc ttgggcggct cctgcgccgg caccggccgg actgcgtggc
      781 cgtcgagagc gtcttccacg ccgcgaacgt ccgcagcgcg ctcacgctcg gccaggcgag
      841 gggcgtcgcg ctgctcgcgg cggtcgaagc gggctacccg ctggtcgagt acgcgccggc
      901 ggaagtcaag ctggcggtcg tcggctacgg ccgggccgag aagaggcagg tgggcgagat
      961 ggtgcggctg ctcctggggc tggtcgaggc gcccgcgccg aacgacgcgt cggacgcgct
     1021 ggccgtggcc atctgccatg tgcacagctg cggcggcgcg gcgctggcgc cggccgcgcg
     1081 cgaggcggcg ccggcggccg ggcgccgacg gcgcgcgcca accagctggc gcgagtaccg
     1141 gccatgatcg cgttcctccg cggccgcctg gccgggaagc agccgaaccg gatcatcgtc
     1201 gacgtcgccg gcgtgggctt cgacgtggcg gtgccgctgt cgacgttcta caagctccag
     1261 gacgagggat cggacgtcgc gctttggatc cacacgcacg tccgcgagga tgcgatcgcg
     1321 ctgtacgggt tctcgaccgg gctcgagcag cagattttcg agcgcctgat cgggatcggg
     1381 ggcatcgggc cgaagctcgc gctcgccgtc ctgtcgggaa tcgacgtggc cgatctcgtg
     1441 cgtgcggtcc gcgccgggga cgtggcgagg ctgacggcca ttccgggcat cggcaggaag
     1501 acggccgagc gcatcgggct ggagctgaag gacaagctgc ccgacacgtt cgccgccgac
     1561 ctggcggccg acgagtccgg cgccggcgcg gacggcggcc tgcggggcga cctgctctcg
     1621 gcgctcctga acctggggta ccatcggcct ctggcggaac gagccgtgga tgcggcgctc
     1681 gctcgcgcgg cctcgccgac cttcgaggcg gcgctcaagc aggcgctgcg cgagctggcc
     1741 cgccgctgag cgatgaccga cgacagcacg cgggtgacgt ccatgcgcgc cgacgacgag
     1801 gacgcgcagt tcgaggcggg cctccggccg cggacgctgg acgagttcac cggccaggac
     1861 cgcgtgcgcg agaacctgca cgtggcgatc acggcggcga agcagcggcg cgaggccctc
     1921 gatcacgtgc tgctctacgg cccgcccggc ctgggcaaga ccacgctggc gtacgtcatc
     1981 gggaacgagc tcggcgtgcc ggtgcgatcg acgtcggggc cggtcatcga gagacccggc
     2041 gacctggccg cgatgctgac gaacctcaag gagcgcgagg tcctcttcat cgacgagatc
     2101 caccgaatgg cggcgacgat cgaggagatc ctctacccgg ccctcgaaga ctacgagctg
     2161 gacatcgtga tcggacaggg gccggccgcg cggtcggtga aggtgccgct ccagccgttc
     2221 acgctgatcg gatcgacgac gcgcgccggg ctcctgacgg ggccgctgcg gggccgcttc
     2281 gggatcgtcc accgcctcga cttctacgcc gacgcggacc tcgacgtgat catccgccgg
     2341 tcggcgcgga tcctcggcgc gccgatcagc ggcgacgccg cggccgagat cgcccggcgc
     2401 tcgcgcggga ccccgcgcat cgcgaaccgc ctgctgcggc gcgtgcgcga ctacgcgcag
     2461 gtgcgggcgc agggcgagat cacggcgccc gtcacgcagg cggcgctggc gatgatggac
     2521 gtggacgagc acggcttcga cgagatcgac cgccggctgc tgcgcacgat catcgacaag
     2581 ttcggcgggg ggccggtcgg cgtcaggacg atagccgccg cgatcagcga ggagcaggac
     2641 gcgatcgagg acatctacga gcccttcctg ctgcaaatcg gcttcatcga tcgcacaccc
     2701 cgcggccgcg tggcgacgcc gcgggcctac gcatacttcg gcttgcaagc cccgggaaag
     2761 gattcgcgac tgtggtgacg cccaacacga tcaggcagac tcggatcggc gcgctcgcga
     2821 cgtacgtgcc gcccaagacg ctcgacaacc accagctgag ccagatggtc gacacgagcg
     2881 acgagtggat cctgcagcgg acggggatca agacccgcca catcgtggag ccaggcgtcg
     2941 cggcctctga cctgggcaag gaagccgcgc tgaaaacgct ggcgcaggcg ggcctcacgc
     3001 cggacgacat cgacgtgatc gtcgtgggca cgacgacgcc ggacatgttc tttcccaaca
     3061 cggcgtcgct catccagacc aagatcgggg ccacgaagtg ctggggcttc gacgtcgcgg
     3121 cggcgtgctc ggggttcact tactcggtgg gcatcgcaag cgacctggtg gccgcgggac
     3181 ggtgcaaccg ggcgctcgtg gtcgggaccg acgtgatgtc ctccatcacc gattacacgg
     3241 accggacgac gtgcgtcctg ttcggcgacg gagctggcgc cgtcatcgtc gagccggccg
     3301 tcgaggaggg attggggatc atcggctgcg agtacgagaa cgacggcacc ctctggcccg
     3361 ccctccagat gccgggcggc ggcagcctcc atccggcttc gcacgagacg gtcgacaaga
     3421 agatgcactt catgaagcag gacggccaga cggtcttcaa gttcgccgtc cgcaagaccg
     3481 aggagatcac gcgcaaggtg ctcgagaagc acggctacac gcccgacgac gtgtcgctgt
     3541 tcgtgtcgca ccaggcgaac cgccggatca tcacgtcggc tggcgagcgc ctcggcctgc
     3601 ccgagtcgaa ggtgatgatg aacatccagg actacggcaa cacgacggcc gccacgatcc
     3661 cgctggcgct cgacgacgcg atccggcagg ggcgcctgaa gaagggcgac ctggtcgtcc
     3721 tcgtgtcggt aggcgcggga ttcaccgtcg gttcggtcct gctgcgctgg gctttctagg
     3781 acaaaggggt cgggtcgatt ttcgacgatc acagaattga ataacaattc tcgt
//
LOCUS       DAOSIF010000298         3832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20963_length_3832_cov_2.851734, whole genome shotgun sequence.
ACCESSION   DAOSIF010000298 DAOSIF010000000
VERSION     DAOSIF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20963_length_3832_cov_2.851734"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PLT35_09955"
     CDS             <1..1263
                     /locus_tag="PLT35_09955"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOG29588.1"
                     /translation="EFRVLERTHADGVRFRFLDCPAYYDRDELYSTPKGDFPDNHLRF
                     ALLARAALECAVKLGEGASVVHAHDWQSGLVPVYLRERCLRHAPLAGAGLVFTIHNLA
                     YQGVFGREVLPSLDLSWDLFTEAGLEFWNKISFLKAGVNFSDVITTVSRRYAQEIQTA
                     EQGFGFEGILRRRADALVGIRNGIDTAAWDPAADPLLPARFSADDLTGKAAVKRALLA
                     RVGLAGPGQWARPIIGMVSRMVDQKGLDLIAGAVNSGELEQLDASFVVLGTGAPQYEA
                     MWRGLAARRPTRFSVVVGFSEELAHMIEGGADLFLMPSRFEPCGLNQMYSLRYGTVPV
                     VRATGGLDDTVEQVDERTGEGTGFKFEPYTSDAMLGELRRALRWFARPAAWRRIQLAG
                     MRQDNSWDASARAYVEVYARARAAAAAR"
     gene            1349..1678
                     /gene="trxA"
                     /locus_tag="PLT35_09960"
     CDS             1349..1678
                     /gene="trxA"
                     /locus_tag="PLT35_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG29589.1"
                     /translation="MASDTVLTLTDGNFDATLQASPVPVLVDFWAEWCGPCRRLAPTI
                     DAVAAELQGRMAVGKLNVDDHPGVSARFGIRGIPTMILFKGGQPVDQIVGLVSKDELT
                     RMVLTHV"
     gene            1778..2698
                     /gene="trxB"
                     /locus_tag="PLT35_09965"
     CDS             1778..2698
                     /gene="trxB"
                     /locus_tag="PLT35_09965"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOG29590.1"
                     /translation="MDTDRDVIIIGSGPAGLTAAVYTARASLKPLLFEGLLAGGQLTT
                     TTEVENFPGFRNGIMGPDLMAEMRAQAERFGTELVAAAVTSVSLSPPPFRVEAEGRTY
                     TAKALIIATGASPRLLGLPEERALMGRGVSTCATCDGFFFRGRPIAVVGGGDSALEEA
                     MFLTKFASKVTLVHRRDALRASKIMQDKALSNPKIEVAWTSVVESINDVGKGEVTGIV
                     LRDVTTGAVREVPVDGVFVAIGHTPNTALFAGQLDLDPAGYVVTRDGTKTSVPGVFAC
                     GDVQDRTYRQAVTAAGSGCMAALDAERYLG"
     gene            complement(2748..>3832)
                     /gene="mnmA"
                     /locus_tag="PLT35_09970"
     CDS             complement(2748..>3832)
                     /gene="mnmA"
                     /locus_tag="PLT35_09970"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG29591.1"
                     /translation="AGGPSNGRRRVVAAMSGGVDSSLAAALLVEAGHEVVGVSMRLCP
                     AQPGMRRSGTCCSAHDLRDAAAVARRLGIPHHVLDLTSRFEATVLANFVAEYAGGRTP
                     LPCAHCNADLKFAALVDATDRLGASWIATGHYARVTRDGAGGVHLWRGADARKDQAYF
                     LFALTARQLSRAMFPIGGMTKGEVRREAQARGFEVADKPDSQEICFVPDGDYADVLDR
                     YLPGDRSGFLADASGRILGRHHGVHRFTVGQRKGLGLATGEPLYVLRIDAERKLVTVG
                     PREALERDWLTASRVNWVAGVEPSFPFRADVQIRSRHAAAPADIALAGPGRVRVDFLS
                     SQAAVTPGQAAVIYRGDEVLGGGWID"
BASE COUNT          563 a         1313 c         1402 g          554 t
ORIGIN      
        1 gagttccgcg tgctggagcg cacgcacgcg gacggcgtgc gcttcaggtt cctggactgc
       61 cccgcgtact acgaccgcga cgagctctac tccacgccga agggcgactt ccccgacaac
      121 cacctgcggt tcgcgctgct ggcccgggca gccctcgaat gcgccgtgaa gctcggcgaa
      181 ggggcgtcgg tcgtgcacgc gcacgactgg cagtcggggc tcgtgccggt ctacctgcgc
      241 gagcggtgcc tcaggcacgc gccgctcgcg ggcgcgggcc tggtgttcac gattcacaac
      301 ctcgcctacc agggcgtgtt cggccgggaa gtgctgccgt cgctggacct ctcgtgggac
      361 ctcttcaccg aagccggcct cgagttctgg aacaagatca gcttcctcaa ggccggggtg
      421 aacttctcag acgtgatcac caccgtgagc cggcgctacg cgcaggaaat ccagacggcc
      481 gagcagggat tcggcttcga ggggatcctc aggcggcggg cggacgcgct cgtcggcatc
      541 cgcaacggga tcgacacggc ggcgtgggac cccgcggccg atccgctcct gccggcaagg
      601 ttcagcgctg acgacctcac gggcaaggcg gcggtcaagc gcgcgctcct ggcccgggtc
      661 gggctggccg ggcccggcca gtgggcgcgg ccgatcatcg gcatggtgtc ccgcatggtg
      721 gatcagaagg ggctcgacct gatcgccggt gccgtgaact ccggggagct ggagcagctg
      781 gacgcctcgt tcgtcgtcct cggcaccggc gcgccgcagt acgaggcgat gtggcgcggc
      841 ctcgcggcca ggaggccgac gcgcttctcc gtggtcgtcg ggttcagcga ggagctggcg
      901 cacatgatcg agggcggcgc cgacctcttc ctgatgccgt cccgcttcga gccctgcggc
      961 ctgaaccaga tgtacagcct gcgctacggg acggtgccgg tcgtgcgggc gaccggcggg
     1021 ctcgacgaca ccgtcgagca ggtggacgag cggaccggcg agggcacggg cttcaagttc
     1081 gagccgtaca cgagcgacgc gatgctcggc gagctccgca gggcgttgag gtggttcgct
     1141 cgcccggccg cgtggaggcg catccagctg gccggcatgc ggcaggacaa ctcctgggac
     1201 gcttcggcgc gggcctacgt ggaggtctac gcgcgggctc gcgcggcagc ggcggcgcgc
     1261 tgaaacggac cgacgagatg tgaatccctg agacgggacg gccgaatcgg ccgccccgca
     1321 ggctcacgac atccgagacg aggaaaacat ggcatccgac acggttctga cgctcaccga
     1381 cggcaatttc gacgcgacgc tgcaggcgtc ccctgtcccc gtactcgtgg acttctgggc
     1441 ggagtggtgc gggccgtgcc ggcgcctggc gccgaccatc gacgcggtgg cggcggagct
     1501 ccagggccgg atggcggtgg gcaagctcaa cgtcgacgat caccccggcg tgtccgcccg
     1561 cttcggcatc cgcggcatcc cgacgatgat tctgttcaag ggcggacagc ccgtggatca
     1621 gattgtgggc ctcgtcagca aggacgagtt gacgcggatg gtcttgacgc atgtctagcg
     1681 atccggaagc cgacattcgg ggcccggagc ctggcgtcgg aagccaggga tctcggcccc
     1741 cggctcacgc ggacaccgat cgcggggaac aggaatcgtg gacaccgatc gcgacgtcat
     1801 catcatcggc tcgggcccgg ctgggctgac ggcggccgtc tacacggccc gcgccagctt
     1861 gaagccgctg ctgttcgaag gcctgctggc cggaggccag ctcaccacca ccacggaggt
     1921 cgagaacttc ccggggttcc ggaacggcat catggggccg gacctgatgg ccgagatgcg
     1981 cgcccaggcc gaacggttcg gaaccgagct ggtggcggcc gcggtcacct cggtcagcct
     2041 gtccccgcct ccgttccgcg tcgaagccga gggccggacg tacacggcga aggcgctgat
     2101 catcgccacg ggggcatcgc cgaggcttct cgggcttccc gaggagcgcg cgctcatggg
     2161 ccgcggcgtc tcgacgtgcg ccacctgcga cgggttcttc ttccgcgggc ggccgattgc
     2221 ggttgtcggc ggcggcgatt cggccctcga ggaagcgatg ttcctcacca agttcgcgtc
     2281 gaaggtgacg ctggtgcacc ggcgcgacgc gctccgcgcc tcgaagatca tgcaggacaa
     2341 ggccctctca aaccccaaga tcgaggtggc gtggacgagc gtggtcgagt cgatcaacga
     2401 cgtcgggaaa ggcgaggtca cgggcatcgt cttgcgggac gtcacgaccg gggccgtgcg
     2461 cgaggtcccg gtggatggcg tcttcgtggc gatcggccac actccgaaca cggcgctgtt
     2521 tgccgggcaa ctggacctcg atcccgccgg ctacgtcgtc acgcgcgacg gcacgaagac
     2581 gagcgtgcct ggcgtgttcg cgtgcggcga cgtccaggac cgcacctacc gccaggccgt
     2641 gacggcggct gggtccggct gcatggcggc gctcgacgcc gagcgctacc tggggtagac
     2701 ggggcaggcg cttcgggcgt gcgagcgtgc atacggcggc ggcctcctca gtcgatccag
     2761 ccgccgccga gcacctcgtc tcctcggtag atcaccgcag cctggcccgg cgtgacggcg
     2821 gcctgcgacg agaggaagtc gacgcgcacc cgtccgggac cggcgagcgc aatgtccgcc
     2881 ggagcagccg cgtgtcggct ccggatctgg acgtccgccc gaaacgggaa cgacggttcg
     2941 acgcccgcca cccagttcac gcgcgacgcg gtcagccagt ctcgctccag ggcctcgcgg
     3001 ggcccgacgg tgaccagctt ccgttccgcg tcgatgcgaa gcacgtagag gggctcaccc
     3061 gtcgccaggc ccaggccttt tcgctgaccg acggtgaagc ggtgcacgcc gtggtggcgt
     3121 ccgaggatcc ggccgctggc gtccgccagg aagcccgatc ggtcgcccgg caggtagcga
     3181 tccagcacgt ccgcgtagtc gccgtccggc acgaagcaga tctcctggct gtcgggcttg
     3241 tcggccacct cgaagccccg cgcctgggct tcgcgccgca cctcgccctt cgtcatgccc
     3301 ccgatcggga acatggctcg cgagagctgg cgcgcggtga gggcgaacag gaagtaggcc
     3361 tggtcctttc gcgcgtccgc gccccgccac aagtgaacgc cgcccgcgcc gtcgcgcgtc
     3421 acgcgcgcgt agtggccggt tgcgatccat gacgcgccaa gccggtcggt ggcgtccacc
     3481 agcgccgcga acttcaggtc ggcgttgcag tgcgcgcacg gcagcggcgt cctgcccccg
     3541 gcgtattcgg ccacgaagtt agcgaggacg gtcgcctcga accggctggt gaggtcgagc
     3601 acgtggtgcg ggatgccgag gcggcgggcg acggcggcgg cgtcgcggag gtcgtgggcg
     3661 ctgcagcatg ttccggaccg ccgcatgccg ggctgggcgg ggcacaggcg catcgacacg
     3721 ccgaccactt cgtggcccgc ttcgacgagg agggcggccg ccagcgacga gtcgacgccg
     3781 ccggacatgg ccgcgaccac ccggcggcgg ccgttggagg gccctccggc ct
//
LOCUS       DAOSIF010000299         3829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_20974_length_3829_cov_4.451245, whole genome shotgun sequence.
ACCESSION   DAOSIF010000299 DAOSIF010000000
VERSION     DAOSIF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20974_length_3829_cov_4.451245"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PLT35_09975"
     CDS             <1..999
                     /locus_tag="PLT35_09975"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG29592.1"
                     /translation="GRDIWAAVRVPDDHLFVGANRARIGEIDLADTANVMASPKLVSF
                     AVEQGWFDPKAGKPFVVNKTYAPDNGIGLTRREWRVIDLVAPSLKLSASLEDYPFSVK
                     PERLLTIEDLRAIQSDYYEGTPYDLTKGPAAGPWGNPIRYGNRGAKGAFERAINTMRT
                     YYLHIGQVNASLPEPFRGTSWFGYGAPDTSYLTPLWPAMNELPAHLGRGDRYGPFDRE
                     SGFWTNIYVQQMAELHYNEAIKHVRAAREPRLSMLYALTPRVLEAAAQMYAKDPKGAI
                     NLITQYGCANAVAWQQDWLRLGDTLLGKYAMGMVDGHPTGYPQWWNEVIGYKPLVR"
     gene            1106..2182
                     /locus_tag="PLT35_09980"
     CDS             1106..2182
                     /locus_tag="PLT35_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29593.1"
                     /translation="MENALTVVVVGCGNMGSAHARAYRADPGFNLVGLVDRVPELRRR
                     LAADLGAVPEYDDLGTALAGAAPDCVAICTYPDTHAALTLQAFEAGCHVFVEKPIAAT
                     VAEAEAVVGAARRTGRKLVVGYILRHHPSWLKFIELARTLGKPLVMRMNLNQQSAGSA
                     WATHKSLMQSMSPIVDCGVHYVDVMCQMTGSTPVRVSAIGARLTEDIPPGMYSYGQLQ
                     VVFADGSVGWYEAGWGPMMSETAFFVKDVVGPLGSVSITSAPGSGGKASADTSSHTKA
                     ESLLLHHAGLDRHGAFERKDEIVELADEPDHLELCAREQRYFRRAIIDDLDLAPHLAD
                     AVNSLRIVLAADESVRTGRTVELR"
     gene            2228..3028
                     /locus_tag="PLT35_09985"
     CDS             2228..3028
                     /locus_tag="PLT35_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOG29594.1"
                     /translation="MRTKLLAAAAVLTAATVGAQAPPPAAPQGPPALPSCAELAAAVQ
                     AAARNDARLQDWAALGRYREANRLLAPPAAGEARVVFMGDSITDSWPQPRFGAFFPGK
                     PYLGRGISGQTTPQMLIRFRPDVIDLQAKVVVVLAGTNDIAGNTGPMTDEQIAGNLAS
                     MAELARAHGIRVVFSSILPTSDYHARPGEVPQTTRRPMARITALNAWMKDYAARNGHV
                     YLDYFSAMIDERGLLRAELSEDDLHPNAEGYAVMAPLAEAAIQAALTR"
     gene            3131..>3829
                     /locus_tag="PLT35_09990"
     CDS             3131..>3829
                     /locus_tag="PLT35_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG29595.1"
                     /translation="MAVLLGDYFPADPDAPSDALLECFLDYVADKGLTLYPAQEEAIL
                     ELLEEKNVILNTPTGSGKSLVASAMLFASLARGQRAVYTCPIKALVNEKWMALCRDFG
                     PDEVGLSTGDASVNRDAPILCCTAEVLGNIALREGENAAIDNVVMDEFHWYADRDRGV
                     AWQTPLLTLPHTRFLLMSATLGDTRFFERALTALNGRPTASVTSDLRPVPLDYAYAER
                     AAFPAAPPAHSAYPG"
BASE COUNT          593 a         1382 c         1318 g          536 t
ORIGIN      
        1 ggccgggaca tctgggcggc cgtgcgcgtc cctgacgacc acctgttcgt cggcgcgaac
       61 cgcgcccgca tcggcgagat cgacctcgcc gacacggcca acgtgatggc gtctccgaag
      121 ctcgtgtcct tcgccgtgga gcaggggtgg ttcgatccga aggccggcaa gccgttcgtc
      181 gtgaacaaga cctacgcgcc cgacaacggg atcggcctga cgcggcgcga gtggcgcgtc
      241 atcgacctgg tcgcgccctc cctgaagctg tccgccagcc tcgaggacta cccgttctcc
      301 gtgaagcccg agcggctgct caccatcgag gacctgcggg cgatccagtc cgactactac
      361 gaggggacgc cgtacgacct gacgaagggc ccggcggccg gcccgtgggg caacccgatc
      421 cgctacggca atcggggcgc gaagggggcc ttcgagcgcg ccatcaacac gatgcgcacc
      481 tactacctcc acatcggcca ggtgaacgcc tcgcttcccg agcccttccg cggcacgagc
      541 tggttcggct acggcgcgcc cgacacctcc tacctgacgc cgctctggcc ggcgatgaac
      601 gaactgcctg cgcatctggg ccggggcgac cgctacggcc ccttcgatcg cgagtcgggc
      661 ttctggacga acatctacgt gcagcagatg gcggagctgc actacaacga ggccatcaag
      721 cacgtgcggg cggcccgcga gccgcggctg agcatgctct acgccctcac cccacgggtg
      781 ctcgaggccg cggcgcagat gtacgcgaag gacccgaagg gcgccatcaa cctgatcacg
      841 cagtacggtt gcgccaacgc cgtcgcctgg cagcaggact ggctcaggct gggcgacacg
      901 ctgctcggca agtacgccat gggcatggtg gacgggcacc cgaccggcta tccgcagtgg
      961 tggaacgaag tgatcggcta caagcccctg gtgcggtagc gccacccgcg ccgcgccggg
     1021 tcgcgcggcg ggccttccgg cggcggcggc cgcagcgaag gccccgcggc cggattccgg
     1081 gcccccgatg gagaggcaca ggcgcatgga gaacgcactg accgtcgtcg tcgtcggatg
     1141 cggcaacatg ggctcggcgc acgcccgcgc gtaccgcgcc gacccgggct tcaacctggt
     1201 tgggctcgtg gaccgcgtcc cggagttgcg ccggcgactg gccgccgacc tcggcgccgt
     1261 cccggagtac gacgatctcg gcacggcgct cgccggcgca gcgccggact gcgtcgcgat
     1321 ctgcacgtat cccgatacgc acgccgcgct gacgctgcag gccttcgagg ccggctgcca
     1381 cgtcttcgtc gagaagccca tcgccgccac ggtggccgag gccgaagccg tggtgggcgc
     1441 ggcgaggcgg accgggcgca agctcgtcgt cgggtacatc ctgcggcacc acccgtcctg
     1501 gctgaagttc atcgagctgg cccgcacgct cggcaagccg ctcgtcatgc gcatgaacct
     1561 caaccagcag agcgccgggt ccgcgtgggc cacgcacaag agcctcatgc agtcgatgtc
     1621 gcccatcgtc gattgcggcg tgcactacgt ggacgtgatg tgccagatga ccggctccac
     1681 gcccgtgcgg gtgagcgcga tcggcgcgcg gctcaccgag gacatcccgc ccggcatgta
     1741 cagctacggc cagctgcagg tcgtgttcgc ggacggctcc gtcggctggt acgaagccgg
     1801 ctggggcccg atgatgagcg agaccgcctt cttcgtgaaa gacgtggtcg ggcccctggg
     1861 ctcggtcagc atcacgtcgg cgcccgggag cggcgggaag gcgtcggcgg acacgagctc
     1921 gcacacgaag gcggagtcgc tcctgctgca ccacgcgggc ctcgaccggc acggggcgtt
     1981 cgagcggaag gacgagatcg tcgaactggc ggacgagccg gatcacctgg agttgtgcgc
     2041 gcgcgagcag cgctacttcc gccgcgccat catcgatgac ctggaccttg cgccgcacct
     2101 ggccgacgcc gtcaacagcc tgcggattgt cctggccgcg gacgagtcgg tgaggacggg
     2161 gcgcacggtc gaactgcgct agcgcggctg ccgtccgccg gacggcggcc aggcagggag
     2221 gaacaccgtg agaacgaagc tgctcgccgc ggccgccgtg ttgacggccg ccaccgtcgg
     2281 tgcgcaggcg ccgcctcctg ccgctccgca gggcccgccc gcgctgccct cgtgcgcgga
     2341 gctggccgcg gccgtccagg cggctgcgcg caacgacgcg cggttgcagg actgggccgc
     2401 cctcggccgc taccgcgagg ccaaccggct gctggccccg cccgccgccg gcgaagcccg
     2461 cgtcgtcttc atgggcgact ccatcacgga ctcgtggccg cagccgaggt tcggcgcctt
     2521 ctttccgggc aagccctacc tcggccgggg catcagcggc cagacgacgc cgcagatgct
     2581 catccggttc aggcccgatg tgatcgacct tcaggcgaag gtcgtggtcg tcctggccgg
     2641 cacgaacgac atcgccggca acaccgggcc gatgaccgac gagcagatcg ccggcaacct
     2701 cgcttcgatg gcggagctgg cccgcgcgca cggcatccgc gtcgtcttct cgagcatcct
     2761 gcccacgagc gattaccacg ctcggccggg agaggttccg cagacgacga ggcggccgat
     2821 ggcgcgcatc accgcgctga acgcgtggat gaaggactac gcggccagga acggccacgt
     2881 ctacctggac tacttctccg cgatgatcga cgagcggggc ctcctgcggg cggaactcag
     2941 cgaagacgac ttgcacccga acgcggaagg ctacgccgtc atggcgccgc tcgcggaggc
     3001 agccatccag gcggcgctca cgcgttagcc gaagaacccg aaggggtcgg gacggatttc
     3061 gcgaggcgac aaaaccgtct tacattcttg gtcgtttgcg gaattcgtcc cgaccccgta
     3121 cgctgggcgg atggcggtgc tgctcggcga ttacttcccg gccgatcccg acgcgccctc
     3181 tgacgcgctg ctcgagtgct tcctcgacta cgtcgccgac aagggcctca cgctctaccc
     3241 ggcccaggag gaggccattc tcgagctgct cgaggaaaag aacgtcatct tgaacacccc
     3301 caccgggtcc ggcaagtccc tggtggcctc ggccatgctc ttcgcctccc tggcgcgcgg
     3361 ccagcgcgcc gtctacacgt gccccatcaa ggcgctggtg aacgagaagt ggatggcgct
     3421 ctgccgcgac ttcgggcccg acgaggtggg gctgtccacg ggcgacgcgt cggtcaaccg
     3481 cgacgcgccg atcctgtgct gcaccgccga ggtcctcggc aacatcgcgc tccgcgaggg
     3541 ggagaacgcg gcgatcgaca acgtggtgat ggacgagttc cactggtacg ccgaccgcga
     3601 ccgcggcgtg gcgtggcaga cgccgctcct gaccctgccg cacacgcggt tcctcctgat
     3661 gtcagccacc ctgggcgaca cgcggttctt cgagcgggcg ctgacggcgc tcaatgggcg
     3721 ccccaccgcg agcgtgacgt cggacctgcg cccggtgccc ctcgactacg cgtacgccga
     3781 gcgcgccgcc ttcccggccg cgccgccggc ccacagcgcc taccccggc
//
LOCUS       DAOSIF010000300         3822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_21027_length_3822_cov_3.828511, whole genome shotgun sequence.
ACCESSION   DAOSIF010000300 DAOSIF010000000
VERSION     DAOSIF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21027_length_3822_cov_3.828511"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLT35_09995"
     CDS             complement(<1..538)
                     /locus_tag="PLT35_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29596.1"
                     /translation="MRRTEAFAVAALAAASLATAQLLPRPAPVEAGLALAVAWAGLLL
                     YVPVRAAAGWCWRQVAAAAPREADRRALGLVLVLGVGLLAWGIDWGLVGHSWAADELR
                     PDWVRDVLRKGLSSGWYDKYPWLHYAVLAVPMAGFEAAEQSGLLAAGGASSWAAQLAV
                     MRAVSVAMGLATLVAAFLC"
     gene            complement(540..2465)
                     /locus_tag="PLT35_10000"
     CDS             complement(540..2465)
                     /locus_tag="PLT35_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG29597.1"
                     /translation="MTDIRSTGAGARAGAAVVLLLLTAGLLAAQGPGATQPPSPSQTP
                     APSQIPSQGPSASPAARPGPGPASKPGEGPAQGPAPGRGPAASILRPGQDARTWPLTR
                     PEATHYAETSRYDDVIGFMKAMAAASPSIRLTVCGYTFEGRPLPLAVVGATDASPGAV
                     RASGKTRIYIQGNIHAGEVEGKEAALWLLRSIAKGERADWLRSVVLLVNPIYNADGNE
                     RVNVRNRYSQFGPVGGMGQRYNAQNLDLNRDNTKMETAEARSMARLLNEYDPHIAIDL
                     HTTNGSAHGYFLTYETSGSPNTSPGIAALVRGDLLPSVTKAVKAAHGWDYFYYGGPTR
                     QGERAWTGDADLYKPRYTQTYFGIRNRIGILSETYSYASFEDRIKATYWFLEAIIDYA
                     VKNGEAIRKTTAAADAESIVGTPQAVRGKLVKTKDAVEIVLAEVAEERNPYVPDVMMR
                     RRVAGSERVEVMPHIGTIEAAETSLAPRAYVVPAASSFPGAPGPGAPDPGGRGLGMGG
                     PFGRAPGGPGTRMLASVIDRLEAHGVKYFVTAKDAPFAGERFRIASSELAQAEYQGTH
                     KLRTLTGQWERVEQTLPAGSLVVPMDQPLARLAFILFDPRSDDGFMAWNILDPVLGAD
                     PAPAFYPVLRTMEHVAK"
     gene            complement(2725..>3822)
                     /locus_tag="PLT35_10005"
     CDS             complement(2725..>3822)
                     /locus_tag="PLT35_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOG29598.1"
                     /translation="DDEQGMRLAVARVLSPYTVRIDDTTGHTVRFAVETADSGEAALE
                     MMAASAPDILLLDMKMGGLSGLDVLHEMRARRIDTLTVMITAYATLETAIEATKRGAH
                     DFLPKPFTPEDLRVAVRRVVRHLMVQREAKRLAEEKRQVRFQFISVLGHELKAPLAAV
                     EGYLQILKDGSAGADPAVIARVVDRALVRTEGMRKLIGDLLNMTRIESGQKRRELVAT
                     DLREAATAAFETASAAAAARGIRLALQAPDRVPIVADRGELDIILNNLVSNAVKYNRD
                     GGRVDVTLEPGAGGGWAIRVADTGIGLTNEDAQRLFQDFVRIKNEHTRSIAGTGLGLS
                     IVKKLAQLYGGTATVQSEPGVGSTFTVTLQG"
BASE COUNT          504 a         1387 c         1349 g          582 t
ORIGIN      
        1 cgcacaggaa cgcggcgacg agcgtcgcga ggcccatcgc aaccgacacg gcgcgcatga
       61 ccgccagctg ggcggcccac gaggaggcgc cgccggcggc gagcaggcct gactgctcgg
      121 cagcttcgaa cccggccatc ggaacggcga gcaccgcgta gtgcagccac ggatacttgt
      181 cgtaccagcc gctcgagaga cccttgcgca ggacgtcgcg cacccagtcc ggccgcagtt
      241 cgtcggcggc ccaggagtgc ccgacgaggc cccagtcgat gccccaggcc agcaggccga
      301 cgccaaggac gagcacgagg cccagggcgc gccggtcggc ctcgcgcggc gcggcagccg
      361 ccacctgccg ccagcaccag ccggcggccg ccctgaccgg cacgtagagg agcaggccgg
      421 cccaggcgac cgccagcgcc agtccagctt cgacaggggc cgggcgcggc aggagctgcg
      481 ccgtggcgag gctggccgcc gccagcgccg ccacggcgaa ggcctctgtc cggcgcatcc
      541 tatttcgcca cgtgctccat cgtgcggagc acggggtaga atgccggcgc cggatccgcg
      601 cccagcacgg ggtccaggat attccaggcc atgaacccgt cgtccgatct cggatcgaag
      661 aggatgaagg ccaggcgggc cagcggctga tccatcggca cgacgagcga cccggcgggc
      721 agcgtctgct cgacgcgctc ccactggccc gtcagcgtcc gcagcttgtg cgtgccctgg
      781 tactcggcct gggcgagttc agacgaggcg atcctgaacc gctcgccggc gaacggcgcg
      841 tccttcgccg tcacgaagta cttgacgccg tgcgcctcga gccggtcgat cacgctcgcc
      901 agcatgcggg tgccggggcc gcccggcgcg cgaccgaagg ggccgcccat cccgaggccg
      961 cggccaccgg gatcgggcgc tcccggccct ggcgctccgg gaaacgagga cgccgccgga
     1021 accacgtacg cccgaggggc caggctcgtc tcggcggctt caatcgtgcc gatgtgcggc
     1081 atcacctcga cccgctcgct gccggccact cggcgccgca tcatcacgtc cggcacgtac
     1141 gggttccgct cctcggccac ctccgcgagc acgatctcga cggcgtcctt cgttttcacc
     1201 agcttcccgc ggacggcctg cggcgtgccg acgatcgact cggcgtcggc cgcggccgtg
     1261 gtcttgcgga tggcctcgcc gttcttcacc gcgtagtcga tgatcgcctc gaggaaccag
     1321 tacgtcgcct tgatgcggtc ctcgaagctc gcgtacgaat acgtctccga caggatcccg
     1381 atccggttgc ggatgccgaa gtaggtctgc gtgtacctcg gcttgtagag atcggcgtct
     1441 cccgtccacg cgcgctcgcc ctggcgcgtg ggcccgccgt agtagaagta gtcccatccg
     1501 tgcgcggcct tgacggcctt cgtcaccgac ggcagcaggt ccccgcgcac gagcgcggcg
     1561 atgcccggcg acgtgttcgg gctcccggac gtctcgtagg tcaggaagta gccgtgggcg
     1621 ctgccgttcg tcgtatgcag gtcgatggcg atgtggggat cgtactcgtt gagcaggcgc
     1681 gccatcgatc gcgcctccgc cgtctccatc ttcgtattgt cgcggttcag gtcgaggttc
     1741 tgggcgttgt agcgctgccc catgccgccc accggcccga actgggagta gcggttgcgg
     1801 acgttcaccc gctcgttgcc gtcggcgttg tagatcgggt tgacgagcag cacgacactc
     1861 cgcagccagt cggcccgctc gcccttggcg atggatcgca gcagccagag ggcggcctcc
     1921 ttgccctcga cctcgccggc gtggatgttt ccctggatgt agatccgtgt cttgcccgat
     1981 gcccgcactg cgcccggcga tgcatccgtg gcgccgacga ccgccaatgg cagcggccgc
     2041 ccctcgaacg tgtagccgca cacggtcagg cggatgctcg gcgaggccgc ggccatggcc
     2101 ttcatgaacc cgatcacatc gtcgtagcgg ctggtctcgg cgtagtgcgt ggcttccggg
     2161 cgggtgagcg gccacgtgcg cgcgtcctgg cccggccgca ggatcgaggc ggcggggccg
     2221 cgccccggcg cgggcccctg cgcgggcccc tcgcccggct tggaggccgg gcccgggccc
     2281 ggccgggccg cgggcgaggc gctcggcccc tggctcggga tctggctcgg ggccggggtc
     2341 tggctcgggg atgggggctg ggtcgcgccc ggcccctggg cagcgagaag cccggctgtc
     2401 agcagcagga ggacaacggc ggctcccgcg cgcgcgccgg ccccggtcga tcggatgtcg
     2461 gtcatctggg cactctccgg aggcccgcgg catgcgcccc gtgcatccgc ggttccgccg
     2521 agtgtagcag atgcctagac cgggccgatc cggcggcttc gcgccgcctt cgccaagcgg
     2581 tctgtgcgcc cggaccgagc gcgcccgagc gggccgaccg gccggctccg gcgcagggca
     2641 agcgttttcc cttgtccctt gttcctttgt tcccggctac tcgccccgag tcccgggtcc
     2701 cgggtcccgg gtcccgagtc cgacctaccc ctggagcgtc accgtgaacg tgctgccgac
     2761 gccgggctcg ctctggacgg tcgcggtgcc gccgtacagc tgggcgagtt tcttcacgat
     2821 cgacaagccg aggccggtgc ccgcgatgga gcgcgtgtgc tcgttcttga tgcggacgaa
     2881 gtcctggaag agccgctggg cgtcctcgtt cgtcaggccg atgcccgtgt ccgccacgcg
     2941 gatggcccag ccgccgccgg cgccaggctc gagcgtcacg tccacccggc cgccgtcgcg
     3001 gttgtacttc accgcgttcg agacgaggtt gttcaggatg atgtcgagtt cgccccggtc
     3061 ggccacgatc gggacgcggt cgggcgcctg cagcgccagg cggatgccgc gggccgccgc
     3121 ggcggccgac gcagtctcga aggcggcggt tgcagcctcg cgcaggtccg tcgcgaccag
     3181 ctccctccgc ttctgcccgg attcgatccg cgtcatgttc aggagatcgc cgatgagctt
     3241 gcgcatgcct tcggtccgga ccagggcccg atccaccacg cgcgcgatca cggcggggtc
     3301 ggcgccggcc gacccgtcct tcaggatctg caggtagcct tcgaccgccg cgagcggggc
     3361 cttcagctcg tgtcccagca ccgagatgaa ctggaagcgg acctgccgct tctcctcggc
     3421 cagccgcttc gcctcgcgct ggaccatcag gtgccggacg acgcgccgga cggcgacacg
     3481 gaggtcctcc ggcgtgaacg gcttcggaag gaagtcgtgg gcgccgcgct tggtggcttc
     3541 gatcgccgtt tcgagcgtcg cgtaggccgt gatcatcacc gtcagcgtgt cgatgcgtcg
     3601 ggcgcgcatt tcgtgcagca cgtccaggcc ggacaggccg cccatcttca tgtcgagcag
     3661 cagaatgtcc ggcgccgagg cggccatcat ctcgagcgcc gcttcgcccg agtcagccgt
     3721 ctcgacggcg aagcggaccg tgtgacccgt cgtatcgtcg atgcggacgg tgtagggaga
     3781 caggacgcgc gccaccgcca ggcgcatgcc ctgctcgtcg tc
//
LOCUS       DAOSIF010000301         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_21146_length_3803_cov_3.695304, whole genome shotgun sequence.
ACCESSION   DAOSIF010000301 DAOSIF010000000
VERSION     DAOSIF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21146_length_3803_cov_3.695304"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..404)
                     /locus_tag="PLT35_10010"
     CDS             complement(36..404)
                     /locus_tag="PLT35_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29599.1"
                     /translation="MSGGRAGRAAAGAGALAALVLLAAACRPPAPRPITIEDRSITIE
                     NQTAETWRNVEVWVNDHYRVTKPELAPRERFAIPMTAFVAGFGQRYDPLRQILKGIEV
                     TATTASGAPVRLSWGTGRRR"
     gene            530..1402
                     /locus_tag="PLT35_10015"
     CDS             530..1402
                     /locus_tag="PLT35_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG29600.1"
                     /translation="MIREIQRDALSGWVMLPVQLAVLVVSVLGFIAAVRPPASAWPVI
                     AWLLVFLANFICWLGFFVVNPNEAKVLQIFGKYVGSVKSPGLRWANPFFSKRRISQRI
                     RNFETTRLKVNDREGNPIEIAAVVVWKVVETAEACFEVDDYNNYVHVQSEAALRNLST
                     SYAYDSHVEHQMSLRGNTAEVAAHLKTEIQDRLAKAGVEVIEARISHLAYAPEIAAAM
                     LQRQQASAIIAARTRIVEGAVGMVEMALDRLNEKAIVTLDEERKATMVSNLLVVLCGE
                     RAAQPVVNTGSLYQ"
     gene            1410..1604
                     /locus_tag="PLT35_10020"
     CDS             1410..1604
                     /locus_tag="PLT35_10020"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HOG29601.1"
                     /translation="MAERKPFLLRLSPEVHESLQRWANDEVRSLNAQIEFLLRRALRD
                     AGRLKPGLPAQTGRRSNGGP"
     gene            complement(1634..1861)
                     /locus_tag="PLT35_10025"
     CDS             complement(1634..1861)
                     /locus_tag="PLT35_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29602.1"
                     /translation="MALKGIAHARLACGCRVSFREGVEGSPVTVMLDLKSPGCGVSWH
                     VTGLPLYDYREALRVPTRFLPPREEGFEEEG"
     gene            complement(1874..2413)
                     /locus_tag="PLT35_10030"
     CDS             complement(1874..2413)
                     /locus_tag="PLT35_10030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG29603.1"
                     /translation="MSTRWMVAAVAAVGLAAVAISGAGRGGWMAGADQSQACDPGAKA
                     ANLGFTLKDLDGRDVSLTSFKGKVVLLDFWATWCGPCKIEIPWFIEFQEKYKPRGFTV
                     VGISSDDTLAKLKPFVAEFAMNYPVLQGLGRDDVHDAFGPVYGLPTTLLISRDGRICK
                     RHMGLKPKAQFEKEILALL"
     gene            2816..>3803
                     /locus_tag="PLT35_10035"
     CDS             2816..>3803
                     /locus_tag="PLT35_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29604.1"
                     /translation="MRKRPTRLAMALWLAAVVALVGVSMAGSAAAQTPYVPYYGKNFV
                     RYDRFDWQIYTTEHFEIYYYTALERHLERVAGYAESAYQHISSELKHDLAFKVPLILF
                     KTHSEFEQQNVVTGAAQEGVGAFAESDHNRVLIPLDDPPDLLYRLIVHELTHVFQFDI
                     VPQSLLRQSMPLWVAEGMSDYMTGVWRPIDLMTIRDAAIADIIPKMSEMEGYGNTSNP
                     RLVYNLGHAALEFIESKWGKEGLRQFLFALRKGIVGGGEGAYKEAFQLSGEEFDQQFD
                     RYLKARFKPFRDKETPADYGRNLAPKPEKTRYGQALTVEPSPSGDLLAAVTLN"
BASE COUNT          610 a         1302 c         1280 g          611 t
ORIGIN      
        1 agcgggcccc gcgccggcct cggcgccgcg cgcgattacc gccgccgccc tgttccccac
       61 gagagcctga cgggcgctcc ggacgccgtt gtcgccgtga cctcgatgcc cttcaggatc
      121 tgccggagcg gatcgtagcg ctggccaaag ccggcgacga acgccgtcat cgggatcgcg
      181 aaccgctcgc gtggggccag ctcgggcttc gtcacgcggt agtggtcgtt gacccagacc
      241 tccacgttcc gccacgtctc ggcggtctgg ttctcgatgg tgatcgaccg gtcctcgatg
      301 gtgatgggcc ggggcgccgg cggacggcat gccgccgcca gcagcaccag ggccgccagg
      361 gcgcccgcgc ccgccgccgc tcggccggct cgtccgccgc tcacgcgcac catcataccc
      421 gcaggcgccg tcctcgcgcg cgccccctct tgatttccga cggcgccccg ggcttgacag
      481 gcagatttca agatgatatc acttagaaag cacgcaagga ggctcctcca tgatccgtga
      541 aatccagcgg gacgcgttgt cggggtgggt gatgctgccc gtccaactcg ccgtactggt
      601 cgtgtcggtc ctcggcttca tcgccgcggt ccgcccgccg gcgagcgcct ggccggtcat
      661 cgcctggctg ctggtcttcc tggccaactt catctgctgg cttgggttct tcgtcgtgaa
      721 ccccaacgag gccaaggtcc tccagatctt cggcaagtac gtcggcagcg tgaagtcgcc
      781 ggggctccgc tgggcgaatc ccttcttctc gaagcggcgc atttcccagc gcatccgcaa
      841 cttcgagacc acgcgcctga aggtgaacga tcgcgagggc aatccgatcg agattgcggc
      901 ggtcgtcgtc tggaaggtgg tggagacggc cgaggcctgc ttcgaggtgg atgactacaa
      961 caactacgtg cacgtccaga gcgaagctgc gctgcgcaac ctgtcgacga gctacgccta
     1021 cgattcccac gtcgagcacc agatgtcgct tcggggcaac acggccgaag tcgcggcgca
     1081 cctgaagacg gagattcagg accggctggc gaaagcgggc gtcgaggtca tcgaggcgcg
     1141 catcagccac ctcgcctacg caccggagat tgcggcggcc atgctccagc ggcagcaggc
     1201 gtcggcgatc atcgcggcgc ggacgcgtat cgtcgagggc gcggtcggca tggtggaaat
     1261 ggcgctcgac cggctcaacg agaaagccat cgtcaccctg gacgaggagc gcaaggcgac
     1321 gatggtgagc aacctgctcg tggtgctgtg cggggagcgg gccgcgcagc ccgtggtgaa
     1381 caccggctcg ctgtaccagt aggaccgccg tggcagagcg caagcccttt ctgctccggc
     1441 tgagcccgga agtccatgag tcgctgcagc ggtgggcgaa cgacgaggtg cgcagcctga
     1501 acgcgcagat cgaattcctg cttcgccgcg cgctccgaga cgcgggacgg ctcaagcccg
     1561 gtctccctgc gcaaacgggc cgccgttcga acggcggccc gtgagtccgg cgaggcccgc
     1621 aaccaggcgc gcctcagcct tcttcctcga atccttcttc cctcggcggc aggaaccgcg
     1681 tcggcaccct cagggcctcg cggtagtcgt agagcgggag gccggtcacg tgccacgaca
     1741 cgccgcatcc cggcgacttc aggtcgagca tgaccgtcac ggggctgcct tcgacgccct
     1801 cgcggaaact cacacggcac ccgcaggcca ggcgcgcgtg tgcaatccct ttcagagcca
     1861 cggtccgatc atactacagg agcgcgagga tctctttttc gaactgggcc ttgggcttca
     1921 agcccatgtg ccgcttgcag atcctgccat cccggctgat gagcagcgtt gtgggcagcc
     1981 cgtacaccgg cccgaaggcg tcgtggacgt cgtcgcgccc gaggccctgg aggacggggt
     2041 agttcatggc gaactcggcg acgaagggct tcagcttggc gagcgtgtcg tcggaggaga
     2101 tcccgaccac ggtgaacccg cggggcttgt acttctcctg gaactcgatg aaccacggga
     2161 tctcgatctt gcacgggccg caccaggtag cccagaagtc gagcaggacc accttgccct
     2221 tgaacgacgt caagctgacg tcccggccgt cgaggtcctt gagcgtgaaa cccaggttcg
     2281 cggccttcgc cccggggtcg caggcctgag actggtcggc tccggccatc caccccccgc
     2341 gcccggcgcc gctgatggcc accgcggcca gccccacggc cgcgactgcg gccaccatcc
     2401 accgtgtgct catcgttctt cccccggcgc cccgttctgg gtcggcgcgc ctccgcagtc
     2461 tcttccgccc cagccggcct gggcccgatc ctggagccgc catggcgtcc gggccgcgct
     2521 ccggaggcca gccagacggg cgtgcgagag cgcctccggg ccgctcgatc cagtctgaca
     2581 gacgggcgtg gcctgaggga agcgcaaagc gagcggcgcg aggcgctccg tccccgttct
     2641 tccaaggatg caggccggac gctccgggat tcacgcttcc gcggtgcgcc ggcggcgcgg
     2701 cctcggccgg ccgcgaggcg ccggcggcat gccggccgtt gcggccgcgt cgtgctgacg
     2761 ctaacatggg tagcggtgtc agctcgcccc tggtaaccgt ccgcgaggtg gtggtatgcg
     2821 caagcgcccg acgcgtttgg cgatggcgct ctggctggct gccgtcgtcg ccctggtcgg
     2881 agtgtcgatg gccgggtccg cggcagccca gacgccgtac gtcccctact acggcaagaa
     2941 cttcgtccgg tatgaccgtt tcgactggca gatctacacg acggagcatt tcgagatcta
     3001 ctactacacc gccctcgaga ggcatctcga gcgcgtggcg ggctacgccg agagcgccta
     3061 tcagcacatc agttcggagc tgaagcacga tctcgcgttc aaggttccgc tgatcctgtt
     3121 caagacgcac agcgagttcg agcagcagaa cgtcgtcacc ggggcggcgc aggaaggcgt
     3181 cggcgcgttc gccgagtcgg atcacaaccg cgtgctgatc cccctcgacg atccgcccga
     3241 cctgctgtac cgcctgatcg tccacgagtt gacgcacgtc ttccagttcg acatcgtgcc
     3301 gcagtcgctc ctcaggcaga gcatgccgct gtgggtggcc gaaggcatgt cggactacat
     3361 gaccggcgtc tggcgcccga tcgacctgat gacgatccgc gacgccgcca tcgcggacat
     3421 catcccgaag atgagcgaga tggaggggta cggcaacacc agcaacccgc ggctggtcta
     3481 caacctggga catgcggccc tcgagttcat cgagtcgaag tgggggaagg aagggctgcg
     3541 gcagttcctg ttcgcgctcc gcaaggggat cgtgggcggc ggtgaaggcg cgtacaagga
     3601 ggccttccag ctgtcgggcg aggagttcga ccagcagttc gaccggtatc tcaaggcccg
     3661 gttcaagccg ttccgcgaca aggagacgcc ggcggactac gggcggaacc tggcgccgaa
     3721 gccggagaag acccggtacg gccaggcgct gaccgtcgag ccgtcgccct cgggcgatct
     3781 gctggcggcg gtgacgctga act
//
LOCUS       DAOSIF010000302         3800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_21164_length_3800_cov_4.265688, whole genome shotgun sequence.
ACCESSION   DAOSIF010000302 DAOSIF010000000
VERSION     DAOSIF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21164_length_3800_cov_4.265688"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLT35_10040"
     CDS             <1..362
                     /locus_tag="PLT35_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG29605.1"
                     /translation="RLRAAPLRTVIVSNSNGRVRAILRRVGLEPLVDLVVDSHEEGVE
                     KPDARLFEIALRKSGADRATTIHCGDLYYVDVTGARAAGLPAVLLDAAGLYADADCPR
                     VASLPEFVDGLLGGAFD"
     gene            604..1920
                     /locus_tag="PLT35_10045"
     CDS             604..1920
                     /locus_tag="PLT35_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG29606.1"
                     /translation="MRSAARLALLLAVPAMAIAAQAQAPVPAPAAAAPPSLLIRPARV
                     FDAVSPAPHEGWAVLVTGNRIAAAGPAAAIKAPAGATTIDLPGTTLMPGLIDAHSHIF
                     LHPYNEATWNDQVVKETLAYRTIAAVIYCRDTLQAGFTSLRDLGTEGAEYADVSVQQA
                     INEGRIPGPRLQVATKAIVASASYGPGPRGFATGVILPRGAEEVSGADQMLWAVRDQI
                     GHGADWVKLYADYRRGPAGATAPTLTLAEMQVAVEEAASAGRPVAVHAAHPEGMRRAI
                     LAGAHTIEHGYGGTDETWALMAERGVALFPTLAAQEASAIYNQGYKPGVSAPTPSLDQ
                     GRRAFQAAMRHGVIIGLGSDVGVFAHGTNARELELMVEYGMTPAQALLAATAVNAKVM
                     GWGDRLGRVQPGYLADLAAFDGDPTRDITAVRRVRFVMKDGIVYRQ"
     gene            complement(2113..>3800)
                     /locus_tag="PLT35_10050"
     CDS             complement(2113..>3800)
                     /locus_tag="PLT35_10050"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOG29607.1"
                     /translation="AGAAAASDKPLDFIRAIVAEDLQAGKHGGRVHTRFPPEPNGYLH
                     IGHAKSICLNFGVALDFGGRCNLRFDDTNPAKEDVEYVDAIRDDVRWLGFDWEGREFY
                     ASDYFEQLYLWAEELIQKGLAYVDNLTADEVREHRGTLTEPGRESPYRDRPAAENLDL
                     FRRMRAGEFADGAYTLRAKIDMASPNINMRDPALYRIRRASHHRTGDAWCIYPMYDFA
                     HALSDAIEGITHSICTLEFEDHRPLYDWCVANCSVPHVPRQIEFARLNVGYTVMSKRR
                     LLALVQEGVVSGWDDPRMPTICGLRRRGYTPESIREFASRVGVAKRENLVDYALLEDA
                     VRDDLNRRAPRAMAVLRPLKVIVENYPEGRAEQIELPRHPDRPEDGVRAIPFSRELYI
                     ERDDFMEDPPKTYFRLAPGREVRLRGTYFLTCRRAVKNEAGEVVELRCTCDPATRGGS
                     APDGRKPKATIHWVSASHAAPAEVRLYDRLFTSLVPGGEADGGDWRASLNPASLDTAA
                     AMVEPSLAGAKPGDRFQFERLGYFCADPDSRPGRPVFNRTVTLKDTWARVAGK"
BASE COUNT          521 a         1403 c         1349 g          527 t
ORIGIN      
        1 agcgcctgcg cgccgcgccg ctccgcaccg tcatcgtctc caactcgaac ggccgcgtga
       61 gggccattct caggcgcgtc ggcctggagc ccctcgtcga tctggtcgtc gactcgcacg
      121 aggagggcgt cgagaagccg gacgcccgcc tcttcgagat cgccctgcgc aagtcgggcg
      181 cggatcgcgc aacgacgatt cactgcggcg acctgtacta cgtcgatgtg acgggcgcgc
      241 gggcggccgg actgccggcc gtgctgctcg acgcggccgg tctctacgcg gacgcggact
      301 gcccgcgggt ggcgtcgctg cccgagttcg tggacgggct gctcggagga gcattcgatt
      361 gaccggcgat cgtccggctg atcggcgcgg gatcccgggg cgttcgcccg aaccatgccg
      421 cgctgcctca gtccgcggaa cccgtcgcag gagccggagc cccggccggg gcgtccagac
      481 gcgccggccc gtcgcaaccc ggcaccccga agaggcttcc tgccggcctg cgtgagcgga
      541 ggcgcagccg ggattccgtg acggtccgtc atgccggccg aaccagccgt cgaggtgaca
      601 cccatgagat ccgccgccag actcgccctg ctgcttgccg ttccggccat ggccatcgcg
      661 gcgcaggccc aggcccccgt gccggccccg gccgccgccg cgccgccgag ccttctgatc
      721 cggcccgcgc gcgtcttcga cgccgtgtcg ccggcgccgc acgaaggctg ggccgtcctc
      781 gtcaccggca accggattgc cgccgccggt cctgccgccg ccatcaaggc gccagccggc
      841 gcgacgacga tcgaccttcc ggggacgacg ctcatgcccg ggctcatcga cgcacactcg
      901 cacatcttcc tgcacccgta caacgaggcg acgtggaacg accaggtcgt gaaggagacg
      961 ctggcctacc gcaccatcgc cgccgtcatc tactgcagag acacgctgca ggccggcttc
     1021 acgtcgctgc gcgacctggg caccgagggc gccgagtacg cggacgtctc ggtgcagcag
     1081 gcgatcaacg agggccgcat tcccggcccg cgcctgcagg tcgccaccaa ggccatcgtc
     1141 gcctccgcca gctacggccc aggcccgcgg gggttcgcca caggcgtcat cctgccgagg
     1201 ggcgccgagg aggtgagcgg cgcggaccag atgctgtggg ccgtgcgcga tcaaatcggc
     1261 cacggcgccg actgggtgaa gctgtacgcc gactaccgcc gcgggcccgc cggcgcgacc
     1321 gcgccgacgc tcaccctcgc cgagatgcag gtggccgtcg aggaggccgc cagcgcgggc
     1381 cggcccgtcg cggtgcacgc cgctcatccc gagggcatgc gccgggccat cctggccggc
     1441 gcgcacacga tcgagcacgg gtacggcggc accgacgaga cgtgggcgct gatggccgaa
     1501 aggggcgtcg cgctgttccc gacgctcgcc gcccaggagg cctccgccat ctacaaccag
     1561 ggctacaagc ccggcgtatc cgcgcccacg ccgtccctcg atcagggccg ccgcgcgttc
     1621 caggccgcca tgcggcacgg cgtcatcatc ggccttggca gcgacgtcgg cgtcttcgcg
     1681 cacggcacca acgcccgcga gctcgagctg atggtcgagt acgggatgac gcccgcgcag
     1741 gccctcctgg cggcgacggc ggtcaatgcg aaggtgatgg gctggggcga cagactgggc
     1801 cgggtgcagc cgggctacct cgccgatctc gccgcattcg acggcgaccc gacgcgcgac
     1861 atcaccgccg tccgccgggt gcggttcgtc atgaaggacg ggatcgtgta cagacagtag
     1921 gggcaagggg ctgggcgccg ggcgccaggt cgattggctt gccgcgccgc gcagccgtct
     1981 cacgaaggcg gggagcaggg cgaagggcgc tgggggctcg tccgacgcga ctgcggtcgc
     2041 gcgggtcgcc cgacctcaca gcacgaccag tcaaccggcc agcgcctggc gcccgggctc
     2101 gagcccctgt cgctacttcc ccgccacccg cgcccacgta tccttcagcg tcaccgtgcg
     2161 gttgaagacc ggccggcccg gcctcgaatc cgggtcggcg cagaagtagc cgaggcgctc
     2221 gaactggaag cggtcgccgg gcttcgcgcc cgcgagcgac ggctcgacca tggcggccgc
     2281 cgtgtcgagc gatgccgggt tcagcgaggc ccgccagtcg ccgccatccg cctccccgcc
     2341 cggcacgagg ctcgtgaaca gccggtcgta gaggcggacc tccgccggcg ccgcgtggga
     2401 cgcggacacc cagtgaatcg tcgccttcgg cttgcggccg tcgggcgcgc tgccgccgcg
     2461 ggtggccggg tcgcaggtgc accgcagctc caccacctcg ccggcctcgt tcttcacggc
     2521 gcgccggcac gtgaggaagt acgtgccgcg cagcctcacc tcgcggcccg gggcgaggcg
     2581 gaagtaggtc ttcggcggat cttccatgaa gtcgtcgcgc tcgatgtaca gctcgcgcga
     2641 gaacggaatg gcgcgcacgc cgtcctccgg ccggtccggg tggcgcggca gctcgatctg
     2701 ctccgcccgg ccttccgggt agttctcgac gatcaccttc agcggccgca gcacggccat
     2761 cgcgcgcggc gcgcggcggt tcaggtcgtc gcggacggcg tcctcgagca gcgcgtagtc
     2821 cacgaggttc tcgcgcttcg ccacgccgac ccgcgaggcg aactcccgaa tcgactccgg
     2881 ggtgtagccg cgccgccgca gcccgcagat ggtcggcatg cgcgggtcgt cccagcccga
     2941 cacgacgccc tcctgcacga gcgcgagcag ccggcgcttg ctcatcacgg tgtagccgac
     3001 gttcaggcgc gcgaactcga tctggcgcgg cacgtgcggc acgctgcagt tggcgacgca
     3061 ccagtcgtag agcggcctgt ggtcctcgaa ctccagcgtg cagatggagt gcgtgatgcc
     3121 ctcgatggcg tccgacagcg cgtgcgcgaa gtcgtacatc gggtagatgc accaggcgtc
     3181 gcccgtccgg tggtgcgagg cgcgccggat gcggtagagc gccgggtcgc gcatgttgat
     3241 gttgggcgac gccatgtcga tcttcgcgcg cagcgtgtag gcgccgtcgg cgaactcccc
     3301 ggcgcgcatc cggcggaaga ggtccaggtt ctccgcggcg ggccggtcgc gatacgggct
     3361 ctcgcgcccc ggctcggtca gcgtcccgcg gtgctcgcgc acttcgtcgg ccgtcaggtt
     3421 gtccacgtag gccaggccct tctggatgag ctcctcggcc cacaggtaga gctgctcgaa
     3481 gtagtccgac gcgtagaact cgcgcccctc ccagtcgaac cccagccacc gcacgtcgtc
     3541 gcggatcgcg tccacgtact cgacgtcctc cttcgccggg ttcgtgtcgt cgaaccggag
     3601 gttgcacctg ccgccgaaat ccagcgcgac gccgaagttc aggcagatcg acttggcgtg
     3661 gccgatgtgc aggtagccgt tcggctcggg cgggaaccgc gtgtggacgc ggccgccgtg
     3721 cttcccggcc tgcaggtcct cggcgacgat ggcgcggatg aaatcgaggg gcttgtcgct
     3781 cgcggccgcg gccccggccg
//
LOCUS       DAOSIF010000303         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_21198_length_3793_cov_3.956394, whole genome shotgun sequence.
ACCESSION   DAOSIF010000303 DAOSIF010000000
VERSION     DAOSIF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21198_length_3793_cov_3.956394"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2508
                     /locus_tag="PLT35_10055"
     CDS             <1..2508
                     /locus_tag="PLT35_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG29608.1"
                     /translation="VEDVRVAIERGNRNFSGGILADGAQETLIKGVGRIASLDDIAAT
                     VVATRRNVPICVRDVAEVRVGPKFSREAASHNGKEAVSVTVEKQYGGDTLTAIGNLRA
                     RLARIAGELPAGVSIEPFYDQSRLIVSSLDHVQLSMLIGAGLIVLVMLFFLWDLRGAL
                     IASLTIPLSMLIALVMMHLGGVGLTVMSIGGLAIGIGKTANGSIIMVENIYRVQRERA
                     GQGPPDALTLAGASEVGPALASANVLIVLVFLPMMTLSGLEGAMFRPTAFAVAAALLG
                     SLVLNLTLQPALCACPFGAHRGPGRPNPVTTFLTRWYGRLLAAALDRKRIVFLPLAAG
                     VIAAAVAVPRLGREFVPPLDEGAILASTVMLPETSLEESVAMGRRVERIIMALPEVAS
                     VTRTTGSAESSEHLHPVGHSHFAIQLKPRGERTRGYAEITAALREPFNQLPGMAYLFE
                     QPISNKLAEMLTGTEGELSVKLFGPDLEVLNRRIEDIRQVLATIDGIEDLQVEQTAGI
                     PQLLVEADRERLSRFGVSVSQVADVIETALNGLDVTDVYEGDRVTSVLMRLPERYRRD
                     EDAVRNLLVDTPGGERVPLSELARIARGQGPQTIFRENLTRRKIAMCNVVGRDVGSVV
                     EEARRRIAAEVPLPPGYYVTFGGQFESQQHAMRHLTIVMALALLVIFVVLFAAFGSVG
                     QALLVILNVPTALAGGILALWFAGESLNVSSTIGLIGLFGVCAQNDIILVSKINDLRR
                     QGLALRDAVVEGSLTKFRAIFMTDLVMIVGVLPLALIEATGSELHRPLAIVYVGGFLF
                     AMVLRRFIVPALYEVFARFERPGCRVPADSGGRGQAG"
     gene            2716..3351
                     /locus_tag="PLT35_10060"
     CDS             2716..3351
                     /locus_tag="PLT35_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG29609.1"
                     /translation="MAKAAFSIAGDRMAPVFDVARPLRIIDAEGGRVVGESGAEFADD
                     VPERRALQLVALGVTELVCGAASRPMHAALAAHGIRVTPFVTGRTRDVVQAWLSGHVT
                     RPAFAMPGCRGRRRRGGLPGRGLRGVDRPFEGAGRGGEGAGWGGGRGQGAGQGRGPRL
                     GRLGGPFAAAAFGTCVCPACGHREPHERGVPCVQTQCPACGTAMTRGVGDE"
BASE COUNT          531 a         1289 c         1399 g          574 t
ORIGIN      
        1 gtggaggacg tgcgcgtcgc cattgagcgc ggcaaccgga acttctcggg gggcatcctc
       61 gccgacgggg cgcaggaaac gctcatcaag ggcgtgggac gcattgcgtc gctcgacgac
      121 atcgcagcga ccgtggtcgc cacgcgccgc aacgtcccga tctgcgtgcg ggacgtggcc
      181 gaggtccgcg tggggccgaa gttcagccgc gaggcggcga gccataacgg gaaggaggcc
      241 gtcagcgtca cggtcgaaaa gcagtacggc ggggacacgc tcacggcgat cggcaacctc
      301 cgggcccggc tggcccggat cgccggcgaa ttgcctgcgg gggtgtcgat cgagcccttc
      361 tacgatcagt ccaggctcat cgtcagctcg ctggaccacg tgcagctgtc catgctgatc
      421 ggcgccgggc tgatcgtgct ggtgatgctg ttcttcctgt gggacctgcg cggggcgctc
      481 atcgcctcgc tgacgatccc gctgtcgatg ctgattgccc tcgtcatgat gcatctgggc
      541 ggcgtcggcc tcaccgtcat gtcgatcggg ggcctcgcca tcgggatcgg gaagaccgcc
      601 aacggctcga tcatcatggt ggagaacatc taccgggtgc agcgcgagcg cgcgggccag
      661 ggcccgcctg acgccctgac gctcgccggc gcgagcgagg tggggccggc cctggcgtcg
      721 gcgaacgtcc tgatcgtgct ggtgttcctg ccgatgatga cgctgtcggg gctcgagggc
      781 gcgatgttcc gtccgacggc gttcgccgtg gccgcggcgc tgctcggctc gctcgtcctg
      841 aacctgacgc tccagcccgc gctctgcgcg tgcccgtttg gcgcgcatcg cggacccggt
      901 cgccccaacc ccgtcacgac gttcctcacg cgctggtacg gccggctgct ggcggcggcg
      961 ctcgatcgga agcggatcgt gttcctgcct ctggcggcgg gcgtgatcgc ggcggccgtc
     1021 gccgtgccgc gccttggccg cgaattcgtc ccgccgctgg acgagggggc catcctggcc
     1081 tcgacggtca tgctcccgga gacgtcgctc gaggagtccg tcgccatggg ccggcgcgtc
     1141 gagcgcatca tcatggcgct gcccgaggtg gcgtcggtca cgcggacgac gggaagcgcg
     1201 gagtcctcgg agcacctcca tcccgtcggg cacagccact tcgcgatcca gctgaagccg
     1261 cgaggagagc ggacgcgggg gtacgcggag atcaccgccg ccctgcgcga gccgttcaac
     1321 cagctgccgg gcatggccta cctgttcgag cagcccatct ccaacaagct ggccgagatg
     1381 ctcacgggca ccgaggggga gctgtcggtc aagctcttcg gcccggacct ggaggtcctc
     1441 aaccggcgca tcgaagacat ccgccaggtg ctggcgacca tcgacgggat cgaagacctg
     1501 caggtggagc agacggcggg catcccccag ctcctcgtcg aggcagaccg tgagcggctg
     1561 agccggttcg gcgtctccgt gtcgcaggtc gccgacgtga ttgagaccgc gctgaacggc
     1621 ctcgacgtca ccgacgtcta cgagggcgac cgcgtgacct ccgtgctgat gcgcctgccc
     1681 gagcgctacc ggcgcgacga agacgcggtc cggaacctgc tcgtggacac cccgggcggc
     1741 gagcgggtgc cgctgtcgga gctggccagg atcgcgcggg gccagggccc gcagacgatc
     1801 ttccgcgaga acctgacgcg ccgcaagatc gccatgtgca acgtcgtcgg gcgcgacgtg
     1861 ggcagcgtcg tcgaagaggc gcgccgccgg atcgccgccg aagtgccgct gccacccggc
     1921 tactacgtga cgttcggcgg ccagttcgaa agtcagcagc acgccatgcg ccacctgacc
     1981 atcgtcatgg cgctcgcgct gctggtcatc ttcgtcgtgc tgttcgccgc cttcggctcc
     2041 gtcgggcagg cgctgctcgt catcctgaac gtgccgacgg cgctcgccgg cggcatcctc
     2101 gcgctgtggt tcgccggcga gtccctcaac gtgtcgtcca ccatcggcct gatcggcctc
     2161 ttcggcgtct gcgcccagaa cgacatcatc ctcgtctcga agatcaacga cctcaggcgg
     2221 caggggctcg cgctccgaga cgccgtcgtc gaaggctcgc tgacgaagtt ccgcgccatc
     2281 ttcatgaccg acctcgtgat gattgtcggc gtgctgccgc tggccctgat cgaggccacc
     2341 gggtcggagc tgcaccggcc gctcgccatc gtctacgtcg gcggcttcct gttcgcgatg
     2401 gtgctgaggc ggttcatcgt gccggcgctc tacgaggtgt tcgcccgctt cgagcggccc
     2461 ggatgccgag tccctgccga ctccggcggg cgcggccagg caggatagag tagcgtaaca
     2521 gctactaatt aagactcata aagtagctgt ctagctactg aagtctcctg cgccgcatgg
     2581 gcgcctccga gggctttccc gcgctgcatc agcgacttag cctgaatagc cggttcccgc
     2641 gacgacgtgg cggcgcaatt gcaggtaggg gtgtcagcgc ggcgccatcg ccgcctgacc
     2701 ccgcgaggac cgctcatggc gaaagctgcc ttctcgattg ccggggaccg gatggctccc
     2761 gtcttcgacg tcgcccgccc gcttcgcatc atcgacgcgg agggcgggcg tgtcgtcggc
     2821 gagtccggcg ccgagttcgc cgacgatgtg cccgagcgcc gggcgctgca gcttgtcgcc
     2881 ctcggcgtga cggagctcgt ctgcggcgcg gcctccaggc cgatgcatgc ggcgcttgcg
     2941 gctcatggga tccgcgtgac gcccttcgtc acgggacgca cgcgggacgt ggtgcaggcc
     3001 tggctcagcg gacacgtcac ccgcccggcg ttcgccatgc cggggtgccg cggccggcgc
     3061 cggcgcggcg gactgccggg ccgaggcctg cgcggagtcg acaggccatt cgagggggcc
     3121 gggcgaggag gagaaggtgc cggctggggc ggcggccggg gacaaggcgc cggacagggc
     3181 cgaggacctc ggctcggccg cctgggcgga ccgtttgcgg ccgcggcatt cggcacctgc
     3241 gtctgcccgg cctgcggcca ccgcgaacct cacgagcggg gcgtgccgtg cgtgcagacg
     3301 cagtgccccg cgtgcggaac ggccatgacg cgtggcgtcg gcgacgagta ggggaagcgg
     3361 ctgggccgcg cgtcggcccg gcgcaggacg ggaaccgcga acgcggctgt atggaaagga
     3421 gcacgatcat gccacgtgga gacggaacgg gaccgctcgg acgcgggcca atgaccggag
     3481 gcggatgggg atggtgcgcc gggtaccctg accgagggta cggcgggcgc gcgggcgcag
     3541 ggcccggccg gggcttcggc cgaggcgccg gtttcggccg aggcggcggc ggcggacgca
     3601 gcggatacgg gcgcggctgg cgcactgagt tcctgaggac cggattgccg ggatgggccc
     3661 gcggccgatg ggccggtccc ccgccgatcg ccgagagtcc gacgctcgag aaacaggcgc
     3721 tgcaggagga ggccgcggcg cttcaggcgg aactcgatcg gatcaaggcc cggctcggcg
     3781 agctcgaggg cgc
//
LOCUS       DAOSIF010000304         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_21271_length_3784_cov_2.528560, whole genome shotgun sequence.
ACCESSION   DAOSIF010000304 DAOSIF010000000
VERSION     DAOSIF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21271_length_3784_cov_2.528560"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLT35_10065"
     CDS             <1..182
                     /locus_tag="PLT35_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29610.1"
                     /translation="SCGGHFREEFQTPDSEALRDDESFTHVAACEYAGEGAQPVRQVE
                     PLTFEYVKPSQRSYK"
     gene            194..955
                     /locus_tag="PLT35_10070"
     CDS             194..955
                     /locus_tag="PLT35_10070"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOG29611.1"
                     /translation="MANMNVTVRVWRQGGAFGEGRLVPYEARDVSPDMSFLEMLDVVN
                     ESLVASGQPPIAFDSDCREGICGMCSLVVNGVPHGPDRGSTVCQLHMRRFKDGDTITV
                     EPWRAAAFPVRRDLIVDRGAFDQIIAAGGYVSVGTGSAPDANAIPIPKDVAESAMDSA
                     ACIGCGACVAACKNASASLFVGAKVSQLALLPQGHPERVARARALVQAMDDLGFGNCS
                     NEGECEAVCPKEISIANIARMRREYLRALVLGTDR"
     gene            1029..2165
                     /locus_tag="PLT35_10075"
     CDS             1029..2165
                     /locus_tag="PLT35_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOG29612.1"
                     /translation="MTSSWRTLAFVCVAAVPLVAGSRPAAVRGPAESSAANRETPAAL
                     LSLSDEELARRVETDVASLGSLSIGSPNSGILVNPVSMPEGPYWQVSPAAEVWGTEET
                     IAAVEAALSKVHEIYPDAPPILVGDISGADGGRLKRHLSHQAGRDIDFGFFFKGAQPA
                     SLVAGTAANLDLPKNWAFVRALVTETDVEAIFLDTHVQRLLYKYALSICEDKEWLDRV
                     FRFPRGYRGAIISHERGHRNHYHVRFYNPVAQELGRRVHPILVQYGVLPPPVYTVRHV
                     VRPGQTMGLLASRYGTSVRAIKQANGMTSTLLRAGRAYRIPVKTAAPPSEPVAVPPRL
                     LPPTTPAVLANAAWATAAPQGRAAGEADPFLVNPAAVVRVSLLF"
     gene            complement(2298..3248)
                     /locus_tag="PLT35_10080"
     CDS             complement(2298..3248)
                     /locus_tag="PLT35_10080"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOG29613.1"
                     /translation="MLNKIALVGGGNIGGVLAQELFQRKLAATVAMVDVKGPDVAKGK
                     CLDIAEGTPIIGSDVKYIAGKDYGVIEGADLVINTAGVPRTVRPDGTYPSREELLATN
                     LQITDAVAGAIRQYCPDAIVVSIANPLDAIVFRLHQKLSPPKHRLMGMAGALDSARYR
                     YFVAQAAGVSVENVEAMVLGGHGDDMVPVRSCCRIAGMPVEKFLDEPTLAGIEARTRK
                     AGGEIVGLIGVSAFWSPAIAALEMVEAIVLDKRKIIASCVLLEGEYGVNGLFVGVPVV
                     LGRNGVERVIEVDLTEAERAAFAKSIAAVKKTTDEVIAAK"
     gene            complement(3277..>3784)
                     /locus_tag="PLT35_10085"
     CDS             complement(3277..>3784)
                     /locus_tag="PLT35_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29614.1"
                     /translation="PEGAAPVAQVSAGLPDGGLSPVLEAATARLGAHAPRALAPAARD
                     PFRFGVPPAARSGRPASGPQPARAEPPADELAGASARGPRAPDVVLEGMAESQVGEAV
                     ERTAIVTVDGDLVLAGLGVVLGNRYEIVALSADTVELEDVVSRARAVCRLKTARPADR
                     AVSGTVGR"
BASE COUNT          553 a         1400 c         1261 g          570 t
ORIGIN      
        1 agtcctgcgg cgggcatttc cgcgaggagt tccagacgcc ggacagcgag gcgctgcgag
       61 acgacgagtc cttcacccac gtcgcggcgt gtgagtacgc cggcgagggc gcgcagcccg
      121 tccggcaggt cgagccgctg acgttcgagt acgtcaaacc gagccagcgc agctacaagt
      181 aggcggggcg gacatggcca acatgaatgt cacggtgcgg gtgtggcggc agggcggggc
      241 gttcggcgaa ggccgcctgg tgccgtacga ggccagggac gtgtcgcccg acatgtcgtt
      301 cctcgagatg ctcgacgtcg tcaacgagtc gctcgtcgcc tcgggccagc cgcccatcgc
      361 gttcgactcg gactgccgtg aaggcatctg cggcatgtgc agcctcgtcg tgaacggcgt
      421 gccgcacggc ccggatcgcg gctcgacggt ctgccagctc cacatgcgga ggttcaagga
      481 cggcgacacg atcaccgtag agccctggcg ggccgcggcg ttccccgtcc gccgggacct
      541 catcgtcgat cgcggggcct tcgaccagat catcgcggcg ggcggctacg tgtcggtcgg
      601 caccggcagc gcgccggatg cgaacgcgat cccgattccc aaggacgtcg ccgaatcggc
      661 gatggactcg gccgcctgca tcggatgcgg cgcctgcgtc gcggcctgca agaacgcgtc
      721 ggcctcgctg ttcgtcggcg ccaaggtgtc gcagctcgcg ctgctgccgc agggccatcc
      781 cgagcgcgtc gcccgcgcca gggccctcgt tcaggcgatg gacgatctcg ggttcgggaa
      841 ctgctccaac gaaggcgagt gcgaggcggt ctgccccaag gagatctcga tcgccaacat
      901 cgcccgcatg cgccgcgagt acctgcgcgc gctcgtgctc gggacggacc gctgagggcc
      961 tcggcccccg gagcgactgc ggccttgact cgtctgcgca gatggataga gtgatcccag
     1021 cgcatcgcat gacgtcttct tggagaaccc tcgcattcgt ctgcgtggcg gccgtgccgc
     1081 tcgtcgccgg gtcgcgcccg gccgccgtcc gcggaccggc ggaatcctcc gccgcgaacc
     1141 gcgagacgcc ggcagccctc ttgagcctct cggacgaaga actcgcgagg cgcgtggaaa
     1201 ccgacgtcgc ctcgctgggc tcgctgtcga tcggctcgcc gaactcgggg atcctggtga
     1261 accccgtctc catgcccgag gggccgtatt ggcaggtctc gcccgccgcc gaagtgtggg
     1321 gcaccgagga gaccatcgcc gccgtcgagg ccgccctgtc gaaggtgcac gagatctatc
     1381 ccgacgcgcc tcccatcctg gtgggcgaca tcagcggcgc ggacggcggg cgcctgaagc
     1441 gccacctctc gcaccaggcg ggccgggaca tcgatttcgg cttcttcttc aagggcgccc
     1501 agccggcgtc tctcgtcgcc ggcacggctg ccaacctgga cctgccgaag aactgggcct
     1561 tcgtccgcgc cctcgtgacg gagacggacg tcgaggcgat cttcctcgac acccacgtcc
     1621 agcgcctgct ctacaagtac gccctgagca tctgcgagga caaagagtgg ctggatcgcg
     1681 tcttccggtt cccgcgcggg tacaggggcg ccatcatcag ccacgagcgc ggccaccgga
     1741 accactacca cgtccgcttc tacaacccgg tggcgcagga gctgggccgg cgcgtccatc
     1801 ccatcctcgt gcagtacggc gtgctgccgc cgccggtgta cacggtgcgc cacgtcgtgc
     1861 gcccgggcca gaccatgggg ctcctcgcgt cccgctatgg cacgtcggtc cgcgcgatca
     1921 agcaggcgaa cggcatgacg tccacgctgc ttcgcgcggg tcgcgcgtac cgcattcctg
     1981 tcaagacggc cgcgccgccg agcgagccgg tcgccgtgcc gccccgcctc ctgccgccca
     2041 cgacgcctgc agtcctcgcg aacgccgcgt gggcgacggc ggcgccccaa ggcagggcgg
     2101 caggcgaggc cgatcccttc ctggtcaacc cggcggccgt cgtccgcgtc tcgctcctct
     2161 tctgaatgcc ctcggggcct tctgcctgcg cacggcccct gcgatttgac gagtacacga
     2221 gaacgcccgt gaggcttctc aacctcacgg gcgcttgcgt ctgccgtcag atacgagtcc
     2281 cgagccccgc gtgtccccta cttcgccgcg atcacttcgt cggtcgtctt cttcaccgcc
     2341 gcaatcgact tggcaaaggc cgcgcgctcg gcctcggtga gatcgacctc gatgacccgc
     2401 tcgacgccgt tcctgccgag cacgaccggc acgccgacaa acaggccgtt cactccatac
     2461 tcgccctcga gcaggacgca cgacgcgatg atcttccgct tgtccagcac gatcgcctcg
     2521 accatctcca gggcggcgat ggccggcgac cagaacgccg acacgccgat gagcccgacg
     2581 atttctccgc cggccttgcg cgtgcgggcc tcgatgccag ccagggtcgg ctcgtcgagg
     2641 aacttctcga ccggcatccc ggcgatccgg cagcagctcc ggacgggcac catgtcatcg
     2701 ccgtgcccgc cgagaaccat cgcttcgacg ttctcgaccg acacgcccgc cgcctgggcg
     2761 acgaagtacc ggtagcgggc cgagtcgagc gcgcccgcca tccccatgag ccggtgcttc
     2821 ggcgggctca gtttctggtg gaggcggaac acgatggcgt cgagcggatt ggcaatcgac
     2881 acgacgatcg cgtccgggca gtactgccgg atggcgccag ccacggcgtc cgtgatctgc
     2941 aggttcgtcg cgagcagctc ctcccggctc gggtacgtcc cgtccgggcg caccgtgcgc
     3001 ggcacgccag ccgtgttgat caccaggtcc gcgccctcga tgaccccgta gtccttcccc
     3061 gcgatgtact tgacgtcgct gccgatgatc ggcgtgcctt ctgcgatgtc gaggcacttg
     3121 cccttggcga catccggccc cttcacatcc accatggcca ccgtggccgc gagcttgcgc
     3181 tggaagagct cctgcgcgag cacgccgccg atgttgcctc cgccaacgag cgcgatcttg
     3241 ttcagcatgc gatcgatcct cccggaatcc gggggcctag cggcccaccg tccctgaaac
     3301 ggcgcggtca gccggtcttg ccgtcttcaa ccggcagacg gcccgcgccc gtgataccac
     3361 gtcctcgagt tccacggtgt cggcggacaa ggcgacgatc tcgtagcgat tcccgagcac
     3421 cacgcccagg ccggcgagca ccaagtcgcc atccaccgtg acgatggcgg tgcgctccac
     3481 ggcttcccca acctgcgact cggccatccc ctcgagcacc acgtcgggcg cccgaggccc
     3541 tcgggccgag gcgccagcaa gctcgtcggc cggcggctcg gcgcgcgccg gctgcggtcc
     3601 cgacgccggg cgtcccgaac gagcagcggg gggcacgccg aagcggaacg ggtcgcgcgc
     3661 ggcgggcgcc agcgcccgcg gcgcgtgcgc gcccaggcgc gcggtggccg cctcgagcac
     3721 gggcgacagg ccgccgtcgg gcaggccggc actcacctgc gccacaggag cggcgccttc
     3781 aggc
//
LOCUS       DAOSIF010000305         3767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_21402_length_3767_cov_3.081627, whole genome shotgun sequence.
ACCESSION   DAOSIF010000305 DAOSIF010000000
VERSION     DAOSIF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21402_length_3767_cov_3.081627"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLT35_10090"
     CDS             <1..202
                     /locus_tag="PLT35_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29615.1"
                     /translation="ESGIRKPQSRTPKPQPPIPAPVPTCEGTFCLGTARVMQLKRAAS
                     LPDFEEAKALLAQVEQALPPRR"
     gene            complement(294..2072)
                     /locus_tag="PLT35_10095"
     CDS             complement(294..2072)
                     /locus_tag="PLT35_10095"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG29616.1"
                     /translation="MTRRTLLAGLAAVVATAALLAGPAVRAGSEPINYADINKIKAEG
                     LQNSQVMELASWLTDVYAPRLTGSPTFLKAAEWTVAKMKEWGLANAKIEPWANRNGFE
                     RGWTNDKFYLSAVAPERFPIPGTPAAWTPGTNGLVSGEVVLVTAMTEAELAPYKGTLR
                     GKWVLTQAAPDVAAYWKPLATRYTAEELAELESWPAPQPEFGVAAPGAGRMGGPGPGP
                     GAPAAQAPQAGGRQGQPGQPAAAAQPGIVPPAAVQAMMRGTAAAAARHAFFKAEGVLG
                     MFTTTPRGHGIYTISGNRASDPAATVPTISIAAEHYGRLARMVAKNVPVVLEADIRNT
                     FTPDPPVFNVVGEIPGTDKADEIVLLGGHFDTWHASPGATDNTAGVAAMLEALRILKT
                     TGVALRRTVRVGLWGGEEQGLLGSREYVSTHFASRQPLPATPGAPPAPAGGRGGFGGP
                     QGPLELKPAYDKLSVYFNIDNGAGAIRGIYLQGNDAVGPIFREWMEPFRSLGMTTVTI
                     RNTGGTDHQAFDAVGLPGFQFIQDELEYETLTHHTNMDTYERLQPNDMRKMATIAAGF
                     AYLAANRDEKLPRKPLTVPVAGRGGR"
     gene            complement(2241..2338)
                     /locus_tag="PLT35_10100"
     tRNA            complement(2241..2338)
                     /locus_tag="PLT35_10100"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2302..2304),aa:Sec,seq:tca)
     gene            complement(2553..2759)
                     /locus_tag="PLT35_10105"
     CDS             complement(2553..2759)
                     /locus_tag="PLT35_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOG29617.1"
                     /translation="MPTGTIARLLIDKGFGFIRDENGLEHFFHRSAVRGAVFELLRDG
                     QRVEFTPEESQKGPRAGDVRLIED"
     gene            complement(2951..3130)
                     /locus_tag="PLT35_10110"
     CDS             complement(2951..3130)
                     /locus_tag="PLT35_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29618.1"
                     /translation="MSDTGLRIRCQRCGTQMELKDPAPGMPWPPEQYWVCPSCARHFW
                     STYPPPAPKGTAAPA"
     gene            3196..>3767
                     /locus_tag="PLT35_10115"
     CDS             3196..>3767
                     /locus_tag="PLT35_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG29619.1"
                     /translation="MIVGIILAAGASARMARPKALLPIGHDTFVTRLVRTLSSAGLDD
                     LVIVAGDDTDAVRSAVTAAGLRARVVENTRRAEGQLSSLLTGLALADRLGVEAALVHL
                     VDIPLVRPATVAAVLEAFRASNAPVVRPAVGNRHGHPVLFARRVFDDLRRADPSAGAR
                     SVIRALAGQVLDVAVDDEGACRDFDTPEDYA"
BASE COUNT          512 a         1357 c         1319 g          579 t
ORIGIN      
        1 ggaatccgga atccgaaagc ctcagtcccg aaccccgaag ccgcagcctc cgatccccgc
       61 tccggttccc acctgcgagg gcaccttctg cctcggcacc gccagggtga tgcagctcaa
      121 gcgggcggcg tccctgccgg atttcgagga ggcgaaagcc ctgctcgcgc aagtcgagca
      181 ggcgcttccg cctcggcgat gaagcgctgc cgggagccgt gccttagccg gcgggtcagc
      241 cggcgggcgt cgatgtcccg ggcagccgca gccccgaatc ccgagtgcgc cccctacctt
      301 ccccctcgtc cggccacggg caccgtgagc ggcttgcgcg gcagcttctc gtcgcggttc
      361 gccgcgaggt acgcgaagcc cgcggcgatg gtcgccatct tccgcatgtc gttcggttgc
      421 agacgctcgt aggtgtccat gttcgtgtgg tgcgtgagcg tctcgtactc gagctcgtcc
      481 tggatgaact ggaatcccgg cagcccgacg gcgtcgaacg cctggtgatc ggtgccgccg
      541 gtgttcctga tggtgactgt tgtcatgccg agcgaccgga acggctccat ccactcgcgg
      601 aagatgggtc cgaccgcgtc gttgccctgg aggtagatgc cgcggatcgc ccccgcgccg
      661 ttgtcgatgt tgaagtacac cgacagcttg tcgtaggccg gcttgagctc gagcgggccc
      721 tgcgggccgc cgaagccccc gcgccctccc gcaggcgccg gcggcgcgcc cggggttgcc
      781 ggcagcggct ggcggctcgc gaagtgggtc gacacgtatt ccctcgagcc caggagcccc
      841 tgctcctcgc cgccccagag gcccacgcgc accgtgcgcc gcagtgccac tcccgtcgtc
      901 ttgaggatgc ggagggcctc cagcatcgca gcgaccccgg ccgtgttgtc ggtcgcgccc
      961 ggcgaggcgt gccaggtatc gaagtgcccg ccgagcagca cgatctcgtc cgccttgtcg
     1021 gtcccgggga tctcgccgac cacgttgaag accggcggat caggggtgaa ggtgttcctg
     1081 atgtccgctt cgaggacgac cgggacgttc ttggccacca tccgcgcgag gcggccgtag
     1141 tgctcggctg cgatggagat ggtcggcacc gtcgccgccg ggtcggacgc ccggtttccg
     1201 ctgatcgtgt agatgccgtg cccgcgaggc gtcgtggtga acatgcccag tacgccttcg
     1261 gctttgaaaa acgcgtggcg ggcggccgcc gcggccgtgc cgcgcatcat ggcctgcacc
     1321 gccgctggcg gcacgatccc aggctgcgcg gccgccgcgg gctgtccggg ctggccctgg
     1381 cgcccgccgg cctgcggagc ctgagcggct ggagcgcctg gccctgggcc gggcccgccc
     1441 atccgacccg cgcccggcgc ggcgacgccg aactccggct gcggcgcagg ccacgattcg
     1501 agctcagcga gctcctctgc cgtatagcgg gtcgccagcg gcttccagta cgcggccacg
     1561 tcgggcgccg cctgcgtcag cacccacttg ccgcgcagcg tgcccttgta gggcgccagc
     1621 tccgcctcgg tcatggccgt cacgaggacc acctcgccgc tcacgaggcc gttcgtgccg
     1681 ggcgtccacg cggcgggcgt gccggggatg ggaaaccgtt ccggcgcgac cgccgacagg
     1741 tagaacttgt cgttcgtcca tccgcgctcg aagccgttgc ggttcgccca gggctcgatc
     1801 ttcgcgttgg ccaggcccca ctccttcatc ttcgcgaccg tccactcggc tgccttgagg
     1861 aacgtcggcg accccgtcag ccgcggagcg tagacgtcgg tcagccagct ggcgagctcc
     1921 atgacctgcg agttctgaag gccctcggcc ttgatcttgt tgatgtcggc gtaattgatg
     1981 ggctcgcttc ccgctctcac ggcggggccg gcgagcaggg ctgccgtcgc gacgacggca
     2041 gcgaggccgg cgagcagagt gcgtcgtgtc atagcgtcct cgttcgttca ggtgccgggg
     2101 atgacgaacc cgccgcgggc gggagtctcc atgttacacg gggagcggcg gactccagcg
     2161 gagaacctgc tccttgccgt cgcaagtcgc gtggcttgcc acgagcgagg ccctcgtgat
     2221 tcacgggctt ggccggatag tggcggaagc gcctgggagt cgaacccagc cctcccgcga
     2281 tcagcgggag gcgaccggtt ttgaagaccg ggagggccac cgggccccgt tcgcttcctc
     2341 gtgacaggcc ggacagcggc tggcgccgcg gccgcttcat gataccgcag ccgcggccgc
     2401 gtccgggccg gaggaggcgg ggcgcgggcg caccgcacac ggctccccga acactcggga
     2461 ccgcagcatc aggttctaca agtagcggag aggagcgcga gggagccggc ctcgccgaag
     2521 gcaaggccgg ccggactggg gtctggcgcc tgctagtcct cgatcaggcg gacatcgccc
     2581 gccctcggac ctttctgcga ttcctctggc gtgaactcca cgcgctggcc gtcgcgcagc
     2641 agctcgaaaa cggcccctcg gacagcgctc cgatggaaga agtgctcgag gccgttctcg
     2701 tctcgaatga acccgaaccc cttgtcgatg aggagacgcg caatcgtccc ggtcggcata
     2761 cgtgaaccct ccgctgcgaa cgccccgggc gcagcccggg cttcgacacc gccagccgcc
     2821 cctccccggc cgcgcagaca gacgtagccc tccgacggcg gcagtcccga attttccgcc
     2881 gcaagtgtag ggagtacgac cggcgaaagt caagccggat ctggcggatc caccaggcaa
     2941 tccccggcaa tcacgccggc gcggctgttc ccttcggagc gggcggggga tacgtggacc
     3001 agaagtgacg ggcgcacgac gggcagaccc agtactgctc gggcggccac ggcatgcccg
     3061 gcgccggatc cttcagctcc atctgggtgc cgcaccgctg gcaccgaatg cgcaggcccg
     3121 tgtcgctcat gcgcggattc tagcacggac gatcccggtt gccgccgcgg acggcggacg
     3181 gtaacatgcc ccgcgatgat cgtcggcatc atcctcgccg cgggcgcctc ggcgcgcatg
     3241 gcgcggccca aggcgctcct gccgatcggc cacgacacgt tcgtgacgcg cctggtccgg
     3301 acgctcagct ccgccggcct cgacgatctc gtgattgtcg cgggcgacga cacggacgcc
     3361 gtccggagcg cggtgacggc agccggcctc cgggcgcggg tggtcgagaa cacccgccgc
     3421 gccgagggcc agctctcgtc gctgctgacg ggcctcgcgc tagccgatcg gctcggcgtg
     3481 gaggccgccc tcgtgcacct cgtggacatt cccctcgtcc gccccgccac ggtggctgcc
     3541 gtgctcgagg cgttccgcgc ctcgaacgcc ccggtggttc gcccggccgt cggcaaccgc
     3601 cacgggcacc cggtgctgtt tgcgcggcgc gtgttcgacg acttgcgcag ggccgacccc
     3661 tcggccgggg cgaggtcggt catccgcgcg ctcgcgggac aggtgctgga cgtggcggtc
     3721 gacgacgagg gggcgtgccg tgacttcgac acccccgagg actacgc
//
LOCUS       DAOSIF010000306         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_21688_length_3730_cov_4.096054, whole genome shotgun sequence.
ACCESSION   DAOSIF010000306 DAOSIF010000000
VERSION     DAOSIF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21688_length_3730_cov_4.096054"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLT35_10120"
     CDS             <1..1227
                     /locus_tag="PLT35_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG29620.1"
                     /translation="LDEIQSAAPPDQLRSGIPLNGGLLKFHSGTLDLTRAWPGFNPGY
                     ADDAIILLADEYQQGHPAEAVILVTRDAAMRCKARARSVSAEDYWSDRPVASPEKFYS
                     GRVRIDVPQEHAGLLSTLHHEQRLPADALGTLCDVTRLLANQCCELHVSGNGKTAWGI
                     YKSHDGSGYLRRVNTKPGERFGPCTPEQACARDLILDDQSLIVSLVGIAGTGKTLIAL
                     LAAYEMLRSGRVSRIEVYRLNAEAGRELGFLPGDLDEKMAPWAKPVIDNLDFVMRRLH
                     GSTRFSNQVADHHGERRDLPHPTVENLLQSKLLEIAPINYLRGRSIHDAAIIVDDGQN
                     LTQEDMKLVLTRAGEGSRVILTGDPDQIDRAEINALSNGLVQVVERFKGQPNFAHLSM
                     HDVVRSRIAEMAAKLL"
     gene            1330..2271
                     /locus_tag="PLT35_10125"
     CDS             1330..2271
                     /locus_tag="PLT35_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG29621.1"
                     /translation="MGLPDLRRYAWLSIAAAVATITLKTLAYVLTGSVGLLSDAVESI
                     VNLAGAVVALAMLIVAARPADEEHAYGHSKAEYFSSGAEGALILFAAGGIAVAAARRL
                     LTPQPIEQVGLGLAVSAAATAVNLAVALVLRRAAARHASITLDADSKHLLTDVWTSAG
                     VIAGVFAVSATGWLALDPILALAVAANIVRIGWGIVWKSAHGLMDAALPAGELAAVKE
                     ALDEHSRDGIEYHALRTRVSGVRRFVSFHVLVPGAWSVHKGHKLLEDIEARIRTAVPN
                     VTVFTHLESLDDPASWQDQDLDRPEPPRAGPGASAAA"
     gene            2273..3070
                     /locus_tag="PLT35_10130"
     CDS             2273..3070
                     /locus_tag="PLT35_10130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG29622.1"
                     /translation="MGGRAVGGVLAAAAVIAAAVAAGSAQRGAERRDGGIVRGPVGER
                     RIALVFTGHEFAEGGPAILDALASRSVRASFFLTGGFLRTPDFAPLIARIVREGHYLG
                     PHSDKHLLYCDWGPDKPTLVTPEAFARDLDDNLREIESAGVPRSAVRYWMPAYEWYNA
                     DIAAWSSRLGLTLVTFTPGTRSNADYTGEDDPNFVSSQRIVDSVLERERAGPDGLDGF
                     ILLLHIGAGPGRADKMHPRVGELLDELARRRYRFVRVDDLLREGGDR"
     gene            3070..>3730
                     /locus_tag="PLT35_10135"
     CDS             3070..>3730
                     /locus_tag="PLT35_10135"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOG29623.1"
                     /translation="MRRILPAGLLAALLGGGWLLAATPPFFPVAVWYGGGTARAPMLE
                     PVGPESERAWRKDLAAMKALGFNTVRTWVEWSAAEPREGEYRFDQLDLVLKLAEDAGL
                     RVIVQVYVDSAPEWVGARYPDGHFVSQGGTAIKPQAAPGFCFDHPGVRRAVVRFFEAI
                     ARRASASPALYAYDLWSEPAVMNWAQPAYVPNAQFCYCPHTVARFREWLKARHGTVER
                     LN"
BASE COUNT          526 a         1351 c         1333 g          520 t
ORIGIN      
        1 ctggacgaga tccagagcgc cgcgccgccg gaccagctcc gctcgggcat cccgctgaac
       61 ggcgggctgc tcaagttcca cagcgggacg ctcgacctga cgcgcgcctg gccggggttc
      121 aacccgggct acgccgacga tgccatcatc ctgctggccg acgaatacca gcaggggcac
      181 ccggccgagg cggtcatcct cgtgacgcgc gacgccgcga tgcgctgcaa ggcgcgggcg
      241 cggtcggtga gcgccgagga ctactggagc gaccggccgg tggcgtcgcc cgagaagttc
      301 tactcggggc gcgtgcgcat cgacgtcccg caggagcacg ccgggctgct ctcgacgctt
      361 caccacgagc agcgcctgcc ggcggacgct ctcggcacgc tctgcgacgt gacccggctc
      421 ctggccaacc agtgctgcga gctgcacgtg tcgggcaacg gcaagaccgc ctggggcatc
      481 tacaagagcc acgacgggtc cggctacctc cgccgcgtca acaccaagcc cggcgagcgc
      541 ttcggcccct gcaccccgga gcaggcctgc gcgcgcgatc tgatcctcga cgaccagtcg
      601 ctcatcgtca gcctcgtggg catcgccggc accggcaaga ccctgatcgc cctgctcgcg
      661 gcgtacgaga tgctccgcag cggcagggtc tcgcggatcg aggtgtaccg cctgaacgcc
      721 gaagcggggc gcgagctggg gttcctgccg ggggacctcg acgagaagat ggccccgtgg
      781 gcgaagcccg tgatcgacaa cctcgacttc gtgatgcggc gcctgcacgg cagcacgcgc
      841 ttcagcaacc aggtggcaga tcaccacggc gagcggaggg acctgccgca cccgaccgtc
      901 gagaacctgc tgcagtcgaa gctgctcgaa atcgcgccga tcaactacct gcgcgggcgc
      961 tccatccacg acgccgccat catcgtggac gacgggcaga acctgacgca ggaagacatg
     1021 aagctggtgc tcacgcgggc gggggagggc tcccgggtca tcctgacggg cgacccggac
     1081 cagatcgacc gggccgagat caacgcgctc tcgaacggcc tggtgcaggt cgtggagcgg
     1141 ttcaagggcc agccgaactt cgcgcacctg agcatgcacg acgtggtgcg gtcgcggatc
     1201 gccgagatgg ccgcgaagct gctgtaggcc gccctcgccc ggagccccgg gcgggccggc
     1261 gctcccgccc gacgcgcctc tcggccgcgc gctggcggcc tgccgcgccg gtcgggtatc
     1321 ctgtggctcg tgggcctgcc ggacctcagg cgctacgcct ggctctcgat tgccgccgcc
     1381 gtcgccacga tcaccctcaa gacgctggcg tacgtcctga ccgggtccgt cgggctcctg
     1441 tccgacgccg tcgagtcgat cgtcaacctg gcgggcgccg tcgtcgcgct cgccatgctg
     1501 atcgtcgccg cgcggccggc cgacgaagag catgcctacg gccacagcaa ggccgagtac
     1561 ttctcgagcg gcgccgaagg ggcgctcatc ctgttcgccg ccgggggcat cgccgtggcc
     1621 gccgcccgcc ggctgctgac gccgcagccc atcgaacaag tcggcctcgg gctggccgtg
     1681 tcggcggcgg cgacggccgt caatctcgcc gtggcgctcg tgcttcgccg cgccgccgcg
     1741 cgccacgcct cgatcacgct cgacgccgat tcgaagcacc tcttgacgga cgtctggacg
     1801 tcggcgggcg tcatcgccgg cgtgttcgcc gtgtcggcga ccggctggct ggccctcgac
     1861 ccgatcctcg ccctggccgt ggccgccaac atcgtcagga tcggatgggg catcgtgtgg
     1921 aagtcggccc atgggctgat ggacgcggcg ctgcccgccg gcgagctggc ggccgtcaag
     1981 gaggcgctcg acgagcactc ccgcgacggc atcgagtacc acgcgctgcg gacgcgcgtg
     2041 tcgggcgtca ggcggttcgt gtccttccat gtgctggtgc cgggggcgtg gagcgtccac
     2101 aaggggcaca agctgctgga ggacatcgaa gcccgcatcc ggacagccgt ccccaacgtg
     2161 accgtcttca cgcacctgga gtccctcgac gacccggcgt catggcagga ccaggatctg
     2221 gatcgcccgg agccgccgcg agcgggcccg ggcgcgagcg cggcggcctg acatgggcgg
     2281 gcgcgcggtc gggggtgtcc tggccgcagc cgcggtgatc gcagcggccg tcgcggccgg
     2341 gagcgcgcag agaggcgccg agcggcgcga cggcggcatc gtgcgcgggc cggtcggcga
     2401 gcggcgcatc gcgctcgtgt tcacggggca cgagttcgcg gaaggcgggc cggccatcct
     2461 cgacgccctc gcgagccgca gcgtgcgcgc ctccttcttc ctcacgggcg gcttcctccg
     2521 gacgcccgac ttcgccccgc tcatcgcccg catcgtgcgg gagggacact acctcggccc
     2581 gcattcggac aagcacctgc tctactgcga ttggggcccg gacaagccga cgctggtgac
     2641 gccggaggcg ttcgcgcgcg acctggacga caacctccgc gagatcgaga gcgcgggcgt
     2701 gcctcgatcc gcggtccggt actggatgcc cgcctacgag tggtacaacg ccgacatcgc
     2761 ggcgtggagc agccgcctgg ggctgacgct tgtgaccttc acgccgggca cacggtcgaa
     2821 cgcggactac acgggagagg acgacccgaa cttcgtttcg tcccagcgca tcgttgacag
     2881 cgtactggag cgcgagcggg cgggtcccga cggcctggac gggttcatcc tgctgctgca
     2941 catcggcgcc gggcccggac gcgccgacaa gatgcacccg cgcgtcggcg agctgctcga
     3001 cgagctggcc cgccggcgct accggttcgt ccgcgtcgac gatctcttgc gcgaaggagg
     3061 ggacaggtga tgcgccgcat cctgccggcc ggcctcctgg ccgccctgct cgggggcggc
     3121 tggctgctgg ccgccacgcc tccgttcttc cctgtcgccg tctggtacgg cggcggaacc
     3181 gcgcgcgcgc ccatgctgga accggtcggg ccggagtccg agcgcgcgtg gcgcaaagac
     3241 cttgcggcga tgaaggcgct cgggttcaac accgtccgca cgtgggtcga gtggagcgcc
     3301 gccgagccac gggaagggga gtaccggttc gaccagctcg acctcgtgct gaagctcgcc
     3361 gaggacgcgg gcctgagggt catcgtccag gtgtacgtgg attcggcccc cgagtgggtc
     3421 ggcgcccggt accccgacgg ccacttcgtc tcgcagggcg gcacggccat caagccgcag
     3481 gcggcacctg ggttctgctt cgaccacccg ggcgtccggc gcgccgtcgt gcgcttcttc
     3541 gaggcgatcg cgcgccgtgc gtctgccagc ccggcgctct acgcctacga cctgtggagc
     3601 gagccggcgg tgatgaactg ggcccagccc gcctacgtgc ccaacgcgca gttctgctac
     3661 tgcccgcaca ccgtcgcgcg cttccgcgag tggctgaagg ccaggcacgg caccgtcgag
     3721 cggctgaacg
//
LOCUS       DAOSIF010000307         3704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_21891_length_3704_cov_3.185804, whole genome shotgun sequence.
ACCESSION   DAOSIF010000307 DAOSIF010000000
VERSION     DAOSIF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21891_length_3704_cov_3.185804"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLT35_10140"
     CDS             complement(<1..858)
                     /locus_tag="PLT35_10140"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOG29624.1"
                     /translation="MIHAGLLHYGHLLLFLGVMAEADAFLIAGAFLAHRGYWGLGPVI
                     ATAILASATANQGWFWLARARGRRFVERKAETDARFQRLQSWLQRRGAVLIPASRFLY
                     GFRVAIPAAYGASGTAPLTFTALDTASALVWGLVVGFAGYAFGGALDALLADVRRYEP
                     AVLAVVFLVAGALAFRGRSRAATVVAALRRPVEAGGEAALRLLLLVHRVGRRLALHPT
                     GRLAAAAVALGALNAITGIAGTRILHVERLSAWLPFEVTHGSRALMVLAGVGLMFLGR
                     TIARRKRLAW"
     gene            1036..1821
                     /locus_tag="PLT35_10145"
     CDS             1036..1821
                     /locus_tag="PLT35_10145"
                     /inference="COORDINATES: protein motif:HMM:NF017835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG29625.1"
                     /translation="MGCRTRAPLLAGWLLTVAVFQALPSAAAAQARMPLVVRGRTIAL
                     RTYGPSSGAPVVVASGDGGWVHLGPQVAALLAGAGYRVLGLDSRQYLEAFTSGSATLE
                     PEDVRRDFGQVAAVAAQGGAAKPILIGVSEGAGLCVLAASDGAVQAAIRGVIALGLPD
                     VNELGWRWRDAVIYLTKGVPREPTFSVSGIIGRVSPLPLAAIHSTGDEFVPVAVVQRL
                     IALAQPPKRLWLVEASNHRFSGNEGEFRRRLLEAVAWVEAPGR"
     gene            complement(1834..3189)
                     /locus_tag="PLT35_10150"
     CDS             complement(1834..3189)
                     /locus_tag="PLT35_10150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG29626.1"
                     /translation="MADIFIADDDRETCQFIAELLSSPDRRIVFESDPAEALRRAANE
                     RFDAVISDINLNAPQSGLDVLRAARRRNPRCQVVLISGFGTLETAVEAVRAGAFDYIS
                     KPFNIAQVKAVVERALAQAQAPDRGEIRREPALAGGLLGRTAPMLDVYKQIALAADAE
                     APVLIIGESGTGKELVAKAIHAHGRRAPKPFVAVNCGAIAETLLESELFGHAKGSFTG
                     AVSDSKGFFEQAQGGTIFLDEIGETSPALQVKLLRAIEEGEIRPVGAARAFALDVRVV
                     AATNVELERAVAEGRFRQDLFYRVSVVVIRVPPLRERRADIPLLVEAFLRQACARTGR
                     ERALSQPALEALAAHDWPGNVRELENTIERLVLFSPGTTIDIADLPAGMGGRSVPVEE
                     RLFDGLPTLDEVERRYLLHVLDAVKGNRTRAADVLGIDRRTLYRMAGRFGIDLAGDVE
                     R"
     gene            complement(3182..>3704)
                     /locus_tag="PLT35_10155"
     CDS             complement(3182..>3704)
                     /locus_tag="PLT35_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG29627.1"
                     /translation="VIVKDLLSRSRRPLRRDRVDLLALLRRLCALVSPALEAAGVRLR
                     FEGEPVPPVEADAVQLELALLNLVSNAIDAMPGGGDLAIRLAGRAGRALIEVSDTGVG
                     MPPELADRVFQPWVTTKAAGHGTGLGLSIARNVIVEHGGSVGVTSVAGEGSVFTVDLP
                     LPSPSATMNPAHG"
BASE COUNT          543 a         1292 c         1359 g          510 t
ORIGIN      
        1 ccacgcaagg cgcttgcgcc tcgcgatcgt ccggccgagg aacatgaggc cgacccccgc
       61 cagcaccatc agcgcgcggc tgccgtgcgt gacctcgaac gggagccacg cgctgagccg
      121 ctcgacatgg aggattcggg tgccggcgat gccggtgatc gcgttcagcg cgccgagggc
      181 caccgccgcc gcggccagac ggccggtcgg gtgcagcgcc aagcggcgcc cgacccggtg
      241 cacgagcagg aggaggcgca gcgccgcctc gccgccggct tccacggggc gccgcagcgc
      301 ggccacgacc gtcgcggctc gcgagcggcc gcggaacgcc agggcgccgg caaccaggaa
      361 gacgacggcg agcaccgcgg gttcgtagcg ccggacgtcc gccagcagcg cgtcgagggc
      421 gccgccgaac gcgtacccgg cgaagcccac caccagaccc cacacgagcg ccgaggccgt
      481 gtcgagcgcc gtgaaggtga gcggtgccgt gccggatgcg ccgtaggctg cggggatcgc
      541 cacccggaac ccgtacagga agcgcgaggc cggaatcagg acggcgcccc tgcgctggag
      601 ccaggactgg agccgctgga aacgggcgtc cgtctcggcc ttccgctcca cgaaccggcg
      661 ccctcgcgcg cgggcgagcc agaaccagcc ctgattcgcc gtcgccgagg cgagaatcgc
      721 cgttgcgatg acagggccga ggccccagta cccgcggtgg gccaggaagg cgccggcgat
      781 caggaaggcg tcggcctcgg ccatcacgcc gaggaacagc agcaggtggc catagtgaag
      841 cagtccagcg tgaatcatcg gcagcgcgtc cttggcgcgg catcaatcct gcttgtcccg
      901 tttgcaatcc cgatgcccct gccgctagcg agcgaacacg ggcgtcctca ggagccgggc
      961 gggccagcgt ggggcgcttt gccccacgat gtggcgaaat ggcaccggcg ggatctcgaa
     1021 atggcaggtg gtcgcatggg atgtcgaacc cgggccccgc tgctggcggg gtggctgctc
     1081 accgtcgctg tcttccaggc gctgccttca gccgccgccg cgcaggcgag gatgcccctg
     1141 gtcgtgcgag gccgcacaat agcgcttcga acgtacggcc cgagctcggg agcgccggtc
     1201 gttgtcgcca gcggcgacgg tggatgggtc cacctggggc cgcaggtggc ggcactgctg
     1261 gcgggagccg gctatcgcgt cctcgggctg gacagccggc agtacctgga ggccttcacc
     1321 tcgggatccg ccactctcga gccggaggac gtgcgccgcg acttcgggca ggtggcggcg
     1381 gtagccgccc agggcggcgc cgcgaagccc atcctgattg gcgtgtcgga gggagcgggc
     1441 ctctgcgtgc tggcggcgtc agacggcgcg gtgcaggcgg ccatccgcgg cgtgatcgcc
     1501 cttgggttgc ccgatgtgaa cgaactcggc tggcgctggc gtgacgcggt catctacctg
     1561 acgaaaggcg tgccccgcga gccgacgttc agcgtgagcg gcatcatcgg gcgggtgtcg
     1621 ccgcttcccc tggccgccat tcactcgacg ggcgacgagt tcgtccccgt ggccgtcgtt
     1681 cagcgcctga tcgcgcttgc gcaaccgccg aaacgcctgt ggctggtcga ggcgtcgaac
     1741 caccgcttca gcggcaacga aggggagttc cggcgccggc ttctcgaggc ggtggcctgg
     1801 gtggaagcgc ccggccggta gcggcggtcc gactcagcgt tcgacgtcgc ccgcgaggtc
     1861 gatgccgaac cgtccggcca tccggtagag ggtgcggcgg tcgatgccga gcacgtccgc
     1921 cgccctcgtg cggttgcctt tgacggcgtc gagcacgtgg aggaggtagc gacgctccac
     1981 ctcatcgagc gtcggaagcc catcgaacag ccgctcttca accggcacgc tgcgcccgcc
     2041 catcccggcg gggaggtccg cgatatcgat cgtcgtgccc gggctgaaga gcaccaggcg
     2101 ctcgatggtg ttctcgagct cccggacgtt gccgggccag tcgtgggccg cgagggcctc
     2161 gagcgctggc tgcgacaggg cgcgctcgcg gccggtgcgg gcgcacgcct ggcgcaggaa
     2221 cgcctccacg agcagcggga tgtcggctcg gcgttcgcgc aggggcggca cgcggatcac
     2281 cacgacgctg acccggtaga agagatcctg gcggaagcgc ccctcagcga cggcccgctc
     2341 cagctcgacg ttggtggcgg cgacgacgcg gacgtcgagg gcgaacgctc tcgcggcgcc
     2401 cacgggcctg atctccccct cctcgatcgc cctgagcagc ttgacctgaa gcgcggggga
     2461 cgtctctccg atctcgtcga ggaagatggt gcctccctgg gcctgctcga agaagccttt
     2521 cgagtcggac acggcaccgg tgaacgagcc tttcgcgtgg ccgaagagct ccgactcgag
     2581 cagcgtctcc gcgatcgcgc cgcagttcac ggcgacgaag ggcttcgggg cgcggcgacc
     2641 gtgggcgtgg atggccttcg ccacgagctc ctttccagtc ccgctctcgc cgatgatgag
     2701 cactggcgcc tcggcatccg ccgccagggc gatctgcttg tagacatcga gcatcggcgc
     2761 cgtgcggccg agcaggccgc cggccagggc cggctcgcgc cggatctctc cacggtcagg
     2821 agcctgcgcc tgggccaggg cccgctccac cacggccttc acctgggcga tgttgaacgg
     2881 tttgctgatg tagtcgaagg cgccggcccg gacggcttcg acggccgtct cgagcgtccc
     2941 gaagccgctg atcaggacga cctggcagcg cgggttgcgc cgtctcgccg cgcggagcac
     3001 gtcgagcccc gattgcggcg cgttcaagtt gatgtcggag atcacggcgt cgaagcgctc
     3061 gttggcggcc cgtctgagcg cctcggccgg gtccgattcg aagacgatcc gccgatccgg
     3121 cgacgacagg agctccgcga tgaactggca cgtttcccgg tcgtcgtcgg cgatgaagat
     3181 gtcagccatg tgcaggattc atcgttgccg agggcgacgg caacgggaga tcgaccgtga
     3241 acacgctgcc ctcgccggca acgctcgtga cgccgacgct gccgccgtgc tcgacgatga
     3301 cgttgcgagc gatgctgagg cccagcccgg tgccgtgccc ggcggctttc gtcgtcaccc
     3361 agggctggaa gacacggtcg gcgagctcgg gcggcatccc gactccggtg tcgctgacct
     3421 cgatgagcgc gcgccccgcc cgtcccgcta ggcggatggc cagatcgccg ccgccgggca
     3481 tcgcgtcgat cgcgttggag acgaggttca ggagcgcaag ttcgagctgc accgcgtcgg
     3541 cctcgaccgg cggaaccggc tcgccctcga agcggagcct gacgcccgcc gcctcgagcg
     3601 cgggactcac gagcgcgcac agccggcgca gcagggcgag caggtcgacg cggtcccgcc
     3661 tcagcgggcg gcgcgaccgg gagagcaggt ccttcacgat gacg
//
LOCUS       DAOSIF010000308         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22038_length_3686_cov_3.280914, whole genome shotgun sequence.
ACCESSION   DAOSIF010000308 DAOSIF010000000
VERSION     DAOSIF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22038_length_3686_cov_3.280914"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..791)
                     /locus_tag="PLT35_10160"
     CDS             complement(90..791)
                     /locus_tag="PLT35_10160"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOG29628.1"
                     /translation="MTGARIFRLAALAGALALWAGSPAAAQIYSFKDANGNQVFSDRV
                     PHGEYRTFAVAGSAFRTTRPALDEFRNRYDSIIELESARTGVRPDLVRAVIQVESGFN
                     PRARSPKGAMGLMQLMPETAARLGVTNPYDPAQNIRGGVEYLRSLLDRYQDETLALAA
                     YNAGPGAVDRYGAAVPPYRETREYVSKVKQVSPVQAVAAARRIIYKTLVLVDGELVPR
                     YSDTKPPSGAYEVVR"
     gene            complement(831..3506)
                     /gene="alaS"
                     /locus_tag="PLT35_10165"
     CDS             complement(831..3506)
                     /gene="alaS"
                     /locus_tag="PLT35_10165"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG29629.1"
                     /translation="MTSGQLRGTFLSFFERHGHRVVASSPLVPGDDPTLLFTNAGMNQ
                     FKDVFLGTDRRDYRRAATSQKCMRVSGKHNDLENVGPSLRHHTFFEMLGNFSFGDYFK
                     EDAIAFAWSLLTDEWRLPPERLYATVFAGDQGIPRDDDAYRYWLRYLPADRVAELGAA
                     DNFWSMGETGPCGRCSEIHYYRGRELPCPEPVCRGVECSCDRFVEIWNNVFMEFDRQP
                     DGSLRPLPAPSIDTGMGLERISAIMQGTLSNYDTDLFVPLLDEIGRMSGRRYGRGMSP
                     ADVSSRVIADHARAMTFLIADGVVPSNEWRGYVLRKIMRRAMRHGNRLGLTEPFLAPL
                     VDVIVAQMGGAYPELRSGHAYVSKVVASEEGRFEAVLAAGLPRLEALLDECAPSGTLG
                     GEAVFRLYDSYGLPVEFIEDTAAQRGLTVDRAGFERALEGQRERARAKSAFGAQRAGQ
                     FSAGDATRAALERTPDTFEGYDTTRIDGVPIVALFDQAGQEVPVLAAGSAGYAALART
                     PFYVESGGQVSDTGRLVTARGDVAAAVTGVLKVPGWPRLHEVRVESGALEKAGAVTAE
                     VEDTLRDATRRNHTATHLLHAALRRVLGTHVKQAGSLVAPDRFRFDFVHFEALTREQI
                     DEIERIVTEEICRNTPVSTEDRATQEAVADGATALFGEKYGERVRVVSVPGFSMELCG
                     GTHCRATGDIGPFVIVQESGIAAGVRRLEGATGLGALRHVQSQRAALSGVAARLNVPP
                     SQAGDAVDRLQADLKRATRETAQLKMKAATGAGAAPGEISEFGDIRLLTRKVTDLDGA
                     ALRELADSLKASLGKGVVILGAASGEKAQFVVSVTSNVTDRLHAGNLVKQLAPIVGGG
                     GGGRPDFAQAGGKRPDRLDDLLAASRDAVGRMLGT"
BASE COUNT          537 a         1271 c         1308 g          570 t
ORIGIN      
        1 ccctgccgtc ctgcaaggcg tcgctcagct gatcgacacc ggctgcggct cgggctcatg
       61 cgcgccgggg cgcgcgccga cacccgggct cagcgaacga cttcgtaggc gcccgacggc
      121 ggcttggtgt cggagtagcg gggcacgagt tcgccatcga ccaggacgag cgtcttgtag
      181 atgatgcggc gggccgcggc cacggcctgc acggggctga cctgcttcac cttgctgaca
      241 tactcgcgcg tctcgcggta gggcggcacg gctgcgccgt aacggtccac ggcgccagga
      301 cccgcgttgt aggcggccag ggccagcgtc tcgtcctggt agcggtcgag cagcgatctc
      361 aggtactcaa cgcctccgcg gatgttctgg gccggatcgt acgggttcgt gactcccagt
      421 ctggcggcgg tctccggcat cagctgcatg agccccatgg cgcctttcgg cgagcgggcc
      481 cggggattga accccgattc gacctgaatg accgcccgga ccaggtccgg cctgaccccg
      541 gttcgagccg actccagctc gataatgctg tcgtaccgat tacgaaattc gtcgagggcg
      601 gggcgtgtcg ttcggaacgc ggagccggct accgcgaagg tgcggtactc gccgtgcggc
      661 acccggtccg aaaacacctg attgccgttg gcgtccttga acgaatagat ctgggccgcg
      721 gccggagagc ccgcccacag ggcgagcgcg ccggcgagtg cggcgagacg gaagatcctg
      781 gcgccggtca tggcttgcct tccgcgtatc ggctttccgg cggcgtgact tcaggtcccg
      841 agcatccggc cgacggcgtc cctggaggct gcgagcaggt cgtccagcct gtcgggccgc
      901 ttgccgccgg cctgggcgaa gtcgggtctc ccgccgccgc ccccgccgac aatcggggcg
      961 agctgcttga cgaggttgcc cgcatgcagt cggtcggtca cgttgctggt cacggacacg
     1021 acgaactggg ccttttcgcc ggaggcggct cccagaatga ccactccttt cccaagggac
     1081 gccttcaagg agtctgccag ttcccgcagg gcggcgccgt cgaggtcggt gacttttcgg
     1141 gtgagcaggc ggatgtcccc gaactcggag atctcacccg gcgccgcgcc tgcgccggtt
     1201 gcggctttca tcttcagctg ggctgtctcc cgggtcgcac gcttgaggtc ggcctggagg
     1261 cgatcgacgg cgtccccggc ctgggagggc ggcacattga ggcgcgcggc gacgcctgag
     1321 agcgccgcgc gctgggactg gacgtgccgc agcgcgccca agccggtcgc gccctcgagg
     1381 cgccgcacac ccgcggcgat gccgctctcc tgcacgatga cgaaggggcc gatgtcgccg
     1441 gtcgctcggc agtgggtgcc gccgcacagc tccatgctga acccaggcac cgacacgacc
     1501 cggacccgct cgccgtactt ctcaccgaag agcgccgtcg cgccgtcggc cacggcctcc
     1561 tgcgtcgccc gatcctcggt cgacacgggc gtgttccggc agatttcctc cgtcacgatc
     1621 cgttcgatct cgtcgatctg ctcgcgggtg agcgcctcga agtgcacgaa gtcgaagcgg
     1681 aagcggtcgg gcgccaccag cgagcccgcc tgcttcacgt gggttccgag cacgcgccgc
     1741 agggcggcgt ggaggaggtg ggtcgcggtg tgattccgcc gggtagcgtc gcggagggtg
     1801 tcctcgacct cggccgtcac ggcgcccgcc ttctcgagcg cgccggactc gacccggacc
     1861 tcgtgcagcc tcggccagcc cgggactttg agcacaccgg tcacggcggc ggcgacgtcg
     1921 ccccgggccg tcaccagccg ccccgtgtct gagacctggc cgcccgattc gacgtagaac
     1981 ggcgtgcggg cgagagccgc gtagccggcc gagccggccg ccagcacggg gacctcctgc
     2041 ccggcctggt cgaagagggc gacgatcggc accccgtcga tccgggtcgt atcgtagccc
     2101 tcgaacgtgt cgggggtccg ctcgagggcc gcgcgggtcg cgtcacccgc agagaactgg
     2161 ccggcccgct gcgcgccgaa cgcgctcttc gcccgcgcgc gctcgcgctg cccctcgagg
     2221 gcccgctcga agcccgctcg gtcgaccgtc aggccgcgct gcgcggcggt gtcctcgatg
     2281 aattccaccg gaaggccgta cgagtcgtag agccggaaga cagcctcgcc tccgagcgtt
     2341 cccgacggtg cgcactcgtc gagcagggct tcgaggcgcg gcagcccggc ggcgagcacg
     2401 gcttcgaaac ggccttcttc gctcgccacg accttcgaca cgtacgcgtg gcccgacctg
     2461 agctccgggt aagcgccccc catctgcgcg acgatgacgt ccaccagcgg ggcgaggaac
     2521 ggctccgtca ggccgaggcg gttcccgtgg cgcatcgccc gccgcatgat cttcctgagg
     2581 acgtagccgc gccattcgtt cgacggcacc acgccgtccg caatcaggaa ggtcatcgcc
     2641 cgcgcatggt cggcgatcac ccggctggac acgtccgccg gactcatgcc gcgcccgtag
     2701 cggcggcccg acatcctccc gatttcgtcg aggagcggca cgaacaggtc ggtgtcgtag
     2761 ttcgacagcg tgccctgcat gatggcggag atgcgctcca ggcccatgcc cgtgtcgatc
     2821 gacggcgccg gcagcgggcg caacgagccg tcaggctgcc gatcgaactc catgaagacg
     2881 ttgttccaga tctcgacgaa ccggtcgcag gagcactcga cgccccggca caccggttcc
     2941 gggcagggca gttcgcgccc acggtagtag tggatctcgg agcacctgcc gcaggggccg
     3001 gtttcgccca tggaccagaa gttgtccgcc gcccccagtt ccgcgacgcg atccgccggc
     3061 aggtatcgca gccagtaccg gtaggcgtcg tcgtctctcg ggatcccctg atcgccggcg
     3121 aacaccgtgg cgtacaggcg ctcgggcggg agccgccact catccgtcag gagcgaccag
     3181 gcgaaggcga tggcgtcttc cttgaaatag tcgccgaagg agaagttccc gagcatctcg
     3241 aagaacgtgt ggtggcgcaa cgaggggccg acgttctcga ggtcgttatg cttgccgctc
     3301 acgcgcatgc acttctggga ggttgcggcc cggcggtagt cgcgccggtc cgtgcccagg
     3361 aacacgtctt tgaactggtt catcccggcg ttggtgaaga gcagggtggg atcgtcgccg
     3421 gggacgagcg gagaactggc cacgactcga tggccgtgcc gttcgaagaa ggacaggaag
     3481 gtgccgcgca attggccgga ggtcatccct tcattctagc aagggcgccg gggttcggga
     3541 ttccaggttc gggctggaga gggcgcgggc agcagcggtc acacgtcgtc gggctcgctg
     3601 gcccggcggt cccagtaggg gcggagggcg atccggatgc ggtcggcggg gaagccgcgc
     3661 cggaggaggg cgctgaacac gcggcg
//
LOCUS       DAOSIF010000309         3661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22222_length_3661_cov_3.779257, whole genome shotgun sequence.
ACCESSION   DAOSIF010000309 DAOSIF010000000
VERSION     DAOSIF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22222_length_3661_cov_3.779257"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..1525
                     /locus_tag="PLT35_10170"
     CDS             356..1525
                     /locus_tag="PLT35_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29630.1"
                     /translation="MRKAAALVTGILVAGWVGAPARAADAQVRARPQPAPATGAVSAL
                     DKVGALRVRADAGDAAAQLNLGLVYAIGQGVPRDYAEAASWYRKSAEQGNALAQLNLG
                     WCYALGQGVPRDPAQAAHWYRKAADQREPTAQLKLGLLYASGEGVPQSDAEAAVWFRK
                     AAEQGDSTAQYRLGLAFSQGRGVERDAAQAVAWLRKAAERGKPAAQFMLGVMFEQGRG
                     VLQNLPEAVAWYRRAAEQEDATAQLNLGACYELGRGVPQDLAQAALWYRRAAEQGEAR
                     AQANLGYAYANGRGVERDYAEAAAWYRRSADQGNASAQFDLGDLYAQGLGVPRSLVEA
                     YRWVSLAASRASNAEDRTHFSSARDQLAAMMTTGDLIEAQQRIREWTAEFESRPR"
     gene            complement(1836..2921)
                     /locus_tag="PLT35_10175"
     CDS             complement(1836..2921)
                     /locus_tag="PLT35_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOG29631.1"
                     /translation="MSLHQHPSAAALALAAALAGAAIAAAGPDRHPALRAVEVLNDGA
                     PYPQCHASTIAETAPGRLVAAWFGGTEEKAPDVGIWVTRFESGAWTVPVEVATGVQPG
                     GLRHPTWNPVLFAPPGRPLLLFYKVGPSPREWWGMVMASRDGGRTWGPARRLPAPLLG
                     PIKNKPVMLPDGSWLSPSSTESAATGWDVHFERSRDHGRTWSFVGPLARGPFGLQGIQ
                     PSVLFHRDGRLQAIARTRNGVLASSWSRDNGATWSALEPTALPNPNSGTDAVTLRDGR
                     QLLVYNPSTPPPGASSGGPRYPLVAAVSDDGVSWANAVVLEDQPMPDGYAYPAVIQSA
                     DNLVHVTYTWNRRRIKHAILDPSALEQ"
     gene            complement(2918..>3661)
                     /locus_tag="PLT35_10180"
     CDS             complement(2918..>3661)
                     /locus_tag="PLT35_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29632.1"
                     /translation="YVQRYIASRSDREARKSIWIGGLTYVPLSALFLFIGTALFAYYT
                     ARPDELPLAYRGAAKADSVFPWFIVAVLPPGITGLLIAAIFAAAMSTVSTSLNSSATI
                     ILNDYYRRFVAPDTTEARSLRLLRATTVAWGAAGTLAALAMTTVRSALDAWWALAGIF
                     GGGTLGLFLLGFVSRRADNRSAGAGVAAGVALIVWMTLSPRSAALPPAWRSPFHEFLI
                     IVFGTACILAVGLALGRRRAAPSSTENHR"
BASE COUNT          517 a         1273 c         1406 g          465 t
ORIGIN      
        1 gcgaggtcgc ggccctgaac gtcagcctcg cgagactgta ggccgcggac cgagccgcgc
       61 gcccggcagg gccgatcgcg cgccggagtc gcggcgcgcg gcttcatggg cgcggggctg
      121 tcgcggcgcc tgcgccgcac ggggcgctgg cgaagggggt ggcggggcgg gccggcgtgc
      181 gtacggttcc cctatcgggt gtgtctggcc aagcgtgctg ccagttggcg tgtgagcacc
      241 tctgcacccg ccgcgccacc gcgctactgg gagtatcggc tcctgcgccg cggacagcag
      301 gccgaccgcc ggtgtagact ctagtcgaac cgaccggcgg cgtcggaggc gtcccatgcg
      361 gaaggcggcg gcgcttgtca cgggcatcct cgtcgcaggc tgggtcggcg cgcccgcgcg
      421 cgccgccgac gcgcaagtgc gggcaaggcc gcagcccgcg ccggcgacgg gggccgtctc
      481 ggccctcgac aaagtcggag ccctccgcgt gcgcgccgac gcgggtgacg ccgccgccca
      541 gctgaacctc ggcctcgtct acgccatcgg ccagggcgtt cccagggact acgcggaagc
      601 ggcgtcctgg taccggaagt cagccgagca gggcaatgcg ctggcccagc tcaatctcgg
      661 ctggtgctac gccctcgggc agggcgtgcc gcgcgatccg gcccaggcgg cgcactggta
      721 tcgcaaggcc gccgatcagc gagagcccac ggctcagctc aagctgggcc tcctgtacgc
      781 ttcaggagaa ggcgtgccgc agagcgacgc cgaggcggcc gtctggttca ggaaagcggc
      841 cgaacaaggc gattccacgg cgcagtacag gctcggcttg gcgttctcgc aagggcgcgg
      901 cgtcgagcgg gacgcggcgc aggcagtggc gtggctccgc aaggccgccg agcgcggcaa
      961 gccagccgcg cagttcatgc tcggcgtgat gttcgagcag gggcgaggcg tcctccagaa
     1021 cctgccggag gcagtggcgt ggtaccggcg cgccgccgag caggaggacg cgacggcgca
     1081 gctgaatctg ggcgcctgct acgaactggg ccgcggggtc ccgcaggacc tcgcgcaggc
     1141 cgccttgtgg taccgccggg cggctgagca gggcgaggcg cgcgcccagg cgaacctcgg
     1201 ctacgcctac gcgaacggcc gcggcgtcga gcgcgactac gcggaggccg ccgcgtggta
     1261 ccgcaggtcg gccgaccagg ggaacgcctc ggcccagttc gatctcggcg acctctacgc
     1321 ccaaggcctc ggcgtgcccc gcagcctcgt cgaggcgtac cgctgggtca gcctggcggc
     1381 gtcacgggcc tcgaacgccg aggacaggac gcacttctcc tcggcgcgcg atcagctcgc
     1441 tgcgatgatg acaaccggcg acctcatcga ggcgcagcag cggatccggg agtggaccgc
     1501 cgagttcgag tcgcggccgc gataggcgaa acgcccggtc gccaccgtgc gggcgcacgg
     1561 gcggtccgtg ctgcgatggc tgcccgcggc ggccgcgatg gcgaagacgg gccgtgcgtc
     1621 gggcccggca cggcccgggt tcgacaagga ccctggcgcc gcggtccggg cggaggcgtg
     1681 cccgttgcgg tccggccacg cggcaagaac ggcgcccggt agcgctggcc tcgaggtcgc
     1741 ccggttcgcc ggccttccgc tgttcgggcg gtctggagcc gcgcggacgt ccgcttcggc
     1801 gatgaccctt gtcgcctctg tctgtgctcc ggcgactatt gctccagcgc cgacggatcc
     1861 aggatcgcat gcttgatgcg ccggcggttc caggtgtagg tgacatgcac gaggttgtca
     1921 gccgactgga tgacggccgg gtaggcgtag ccgtcaggca tcggctggtc ctcgaggacc
     1981 acggcgttcg cccaggagac gccatcgtcg gaaacggcgg cgaccagcgg gtacctcggg
     2041 cctcccgacg aggcgcctgg cggcggagtc gaggggttgt agacgagcag ctggcgcccg
     2101 tcgcggaggg tgacggcgtc ggttccggaa ttcgggttcg gcagcgccgt cggttcgagc
     2161 gcgctccagg tcgcgccgtt gtcgcgcgac cagcttgatg ccagcacgcc gttgcgcgtc
     2221 cgcgcaatcg cctggaggcg cccgtcgcgg tggaagagca cgctcggctg gatcccctgc
     2281 aggccgaatg gcccgcgcgc gagcggcccc acgaacgacc aggtccggcc gtggtctcgc
     2341 gatcgctcga agtgcacgtc ccacccggtg gcggcgcttt cagtgctcga cgggctcagc
     2401 cacgatccgt ccggcagcat gacgggcttg ttcttgatgg ggccgagcag gggagcgggc
     2461 aggcggcgcg ccggtcccca ggtcctgccg ccgtcgcgcg aggccatcac catcccccac
     2521 cactccctgg gcgacggccc taccttgtag aagagcaaca gcggccgtcc cggcggagcg
     2581 aacaggacgg ggttccacgt cgggtggcgc aggccgccgg gctgcacgcc ggttgcgacc
     2641 tcgaccggca cggtccaggc gcccgactcg aaccgcgtca cccagatgcc gacgtccggg
     2701 gccttctcct ccgtgccccc gaaccaggcc gccacgagcc gtcccggggc ggtttcggcg
     2761 atggtggacg cgtggcactg cggataaggc gctccatcgt tcaggacctc gacggctcgc
     2821 agcgcgggat ggcggtccgg accggcggcg gcgattgccg cgcctgccag cgcggccgcc
     2881 agggccagcg ctgctgcgga gggatgctgg tgcaggctca tcgatggttc tccgtggaag
     2941 acggcgccgc ccggcgccgg ccgagcgcga gcccgacggc gaggatgcac gcggtgccga
     3001 agacgatgat gaggaactcg tggaaggggc tccgccacgc gggcggcagg gccgccgagc
     3061 gcggcgacag ggtcatccac acgatcaggg cgacgccggc ggcgacgccg gcgccggcgg
     3121 agcggttgtc cgcgcggcgc gacacgaacc ccagcaggaa cagccccagc gtcccgccgc
     3181 cgaagatgcc ggcgagcgcc caccacgcgt cgagcgcgct gcgcaccgtc gtcatggcga
     3241 gcgccgcgag cgtcccggcg gcgccccacg cgaccgtcgt ggcccggagg agccgcagcg
     3301 accgcgcctc cgtcgtgtcc ggcgcgacga agcgccggta gtagtcgttc aggatgatcg
     3361 tcgccgacga gttcaggctg gtggagacgg tgctcatggc tgccgcgaaa atcgccgcga
     3421 tgagcaggcc ggtgatcccg ggcggcagga cggccacgat gaaccacggg aacacggagt
     3481 cggccttcgc agcgccgcgg taggcgaggg gcagctcgtc ggggcgggcc gtgtagtagg
     3541 cgaagagggc cgtgccgatg aacaggaaca gcgccgagag cggcacgtag gtgaggccgc
     3601 cgatccagat gctcttgcgc gcctcccggt cggacctcga ggcgatgtac cgctggacgt
     3661 a
//
LOCUS       DAOSIF010000310         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22243_length_3659_cov_3.841010, whole genome shotgun sequence.
ACCESSION   DAOSIF010000310 DAOSIF010000000
VERSION     DAOSIF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22243_length_3659_cov_3.841010"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLT35_10185"
     CDS             complement(<1..517)
                     /locus_tag="PLT35_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29633.1"
                     /translation="MSHHALDRRGFLKTTVGSAAAMAAFPAGLPAARPEGWLPLDHPL
                     PQTATRGDGAQAGVQQSKNPVLKFAVVGINHNHIYAQVNAILRARGELVWLYAREPDL
                     VAAFQQQYPQARLARSEAEVLEDSSIKLVLSSGIPNERAPLGIRVMQHGKDYMSDKPG
                     ITTLAQLAEVRR"
     gene            1238..2230
                     /locus_tag="PLT35_10190"
     CDS             1238..2230
                     /locus_tag="PLT35_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG29634.1"
                     /translation="MDINRRRLLQLGFAAASSAVPVAALAEQPDEAAAAEAKAADGAS
                     HVGVLYDTTLCIGCRECEAACNRRNNLPRTPVPFSDREVLRTARRPSDTAFTVVNQYQ
                     GSPSADQASLPHTYCKVQCMHCLYPSCVSACIVGALTKAADGAVVYNPTICLGCRYCQ
                     IACPFEIPAYEFFKPLTPRVRKCELCTDHEKGTGANPACAAACPTEALVFGRRADLLA
                     TAKERIAKRPARYLNRVYGEREVGGTAWMYLVGRPVREIGLLPLPDKAPPARTEAIQH
                     GIFRYGLLPTAFYGLLAGIMWFNHRAHPEDGPGIGEASDDAAQQGAAPDEEVRR"
     gene            2227..>3659
                     /gene="hybB"
                     /locus_tag="PLT35_10195"
     CDS             2227..>3659
                     /gene="hybB"
                     /locus_tag="PLT35_10195"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HOG29635.1"
                     /translation="MTAPTPPPAPTVPVHDDRPHRVTKPFFTFGTVSLLIAMGVGYSF
                     GVTRMLLGLGQVTNLDDHNPWGIWISFDVACGVALAAGGFTTAALTDIFGGRKYKALL
                     RPAILTAFLGYLWVAIALSFDLGRYWNIWRPIFNWQGNSVLFEVGMCVTVYLCVLAIE
                     MSPSILEGLKARIDDNEWGAAFLRRIEGPIMAAYRGVRVALPIVIVAGVVLSCMHQSS
                     LGTLMLIAPTKMSPLWDTSVLPLLFLFSAFMVGFPMVILESIYANISFGRSPEMELLT
                     PLSRIIPWFIGLYALLKLGDLAVRWDSLDFLARPGATTSLVVEILVGVVAPFLLLLNK
                     AVRRSMGWLFLSVALIIFGVVLNRINVFLVAYEAPYSTRSYFPSVGEIAMTIAIVSSI
                     LFCYRFFVTFFPILPGFQPQRVAELERLREERARTVSPFWTWVIRGSAIAFLLAFVAI
                     YNVVHVTAERGTVKLVDGTNVYLATTSG"
BASE COUNT          521 a         1233 c         1303 g          602 t
ORIGIN      
        1 cgcggcgcac ctcggccagc tgcgcgagcg tcgtgatgcc gggcttgtcg gacatgtagt
       61 ccttgccgtg ctgcatgacc cggatgccga gcggcgcgcg ctcgttcggg atgcccgagc
      121 tcagcaccag cttgatcgac gagtcctcga ggacctcggc ttcgctcctg gcgagcctgg
      181 cctgcgggta ctgctgctga aacgcggcca cgaggtcagg ctccctggcg tacagccaca
      241 ccagttcgcc gcgggcccga aggatggcgt tcacctgcgc gtagatgtgg ttgtggttga
      301 tcccgaccac ggcgaacttc aggaccgggt ttttcgactg ctgcacgccg gcctgcgcgc
      361 cgtcgccgcg ggtagcggtc tgggggagcg gatggtccag cggcagccac ccttcaggac
      421 gcgccgcggg caggccggcg gggaaggcgg ccatcgccgc cgccgatcca accgtcgtct
      481 tcaggaaacc gcgtcgatcg agcgcgtggt gtgacatgga tcactccagg aacagcaggc
      541 ggcaggatac ggcgactgcg gtgaccgggt caacgcggag agcgacggct tcgcccgcgg
      601 actctcaacc ccgaggctcg gcgttgcaga aggagggttc gcgggggaca gaatgcggtg
      661 gctggcagtc gcgttcttga ccctgtcagt ctcacgcgcc gtcggggtga gcgggccgct
      721 ggccgccgcg gtcggcctgc acaggcaggc cgagaacgac ctgctggcgg gaaccagtca
      781 ggaggaaggc ggcgacgccc cctgctctgg aggaggtccc ggttcccggg ggatgcaaca
      841 tctcaggctg cacggggacc ccgaccggcc gcacggcgga ctgcgaggcg gggtactgcg
      901 cgtgctgctt ctgcagcaca ggcgcagtct gccgtcatat gccgacagga gcccgatcag
      961 gctgtgggct gcgcgaggcc ctcggcgggc gcgaggaggg ctgcggcgag cagcatcgcg
     1021 gcggcccgcc aggaaggagc cgggtgcgga cgactgtcag gtcggcgggg cccgccggct
     1081 gggcccgccg cgaggccgcg cactttttcc tttgacggcg cgccggcagc cgggataatc
     1141 agcgaaattc ccggaggggc ggtcaccccc ccggacccgg ggacaggcgc actcatcctg
     1201 gagggcgggc ggccgcgcag gggcgcccgc atcatgcatg gacatcaata gacgccggct
     1261 cttgcaactg gggttcgccg ctgcctcgtc ggccgtgccg gtcgcggccc tggcggagca
     1321 gcctgacgag gccgcggctg ccgaggccaa ggcggcggac ggcgccagcc acgtcggggt
     1381 gctctacgac acgacgctgt gcatcggctg ccgcgaatgc gaggcggcct gcaaccgccg
     1441 caacaacttg ccccgcacgc ccgtgccgtt ctccgaccgg gaggtcctca ggacggcgcg
     1501 gcggccctcg gacacggcat tcaccgtcgt gaaccagtac cagggcagcc cgtccgcgga
     1561 ccaggcttcg ctgccacaca cctattgcaa agttcagtgc atgcactgcc tgtacccctc
     1621 ctgcgtgtcg gcgtgcatcg tcggcgcgct gacgaaggcg gcggacggcg cggtcgtgta
     1681 caacccgacc atctgcctgg gctgccggta ctgccaaatc gcgtgcccct tcgagattcc
     1741 cgcctacgag ttcttcaagc ccctcacgcc gcgggtgcgg aagtgcgagt tgtgcaccga
     1801 ccatgagaag ggcaccgggg ccaatccggc gtgcgccgcg gcgtgcccga cggaggcgct
     1861 cgtgttcggg cggcgcgcgg acctgctggc cacggccaag gagcggatcg ccaaacggcc
     1921 ggcgcgctac ctcaatcgcg tttatggcga gcgcgaggtc ggcggcacgg cgtggatgta
     1981 cctggtcggg cggccggttc gggaaatcgg cctgctcccg ctcccggaca aggcgccgcc
     2041 ggcgcggacc gaggcgatcc agcacgggat cttccggtac ggcctgctgc cgacggcgtt
     2101 ctacggcctg ctggcgggca tcatgtggtt caatcaccgc gcgcatccgg aggacggacc
     2161 gggcatcggc gaagccagtg acgacgccgc gcagcagggc gcggccccgg acgaggaggt
     2221 gcggcgatga cggccccgac gccgccgccg gccccgacgg tgccggtgca cgacgaccgt
     2281 cctcatcgcg tcacgaagcc gttcttcacg ttcggcacgg tgtcgctgct catcgcgatg
     2341 ggcgtcggct actcgttcgg cgtgacgcgc atgctgctcg gcctcggcca ggtgacgaac
     2401 ctcgacgacc acaacccgtg gggtatctgg atctcgttcg acgtggcgtg cggcgtcgcg
     2461 ctggcggcgg gcggcttcac gacggcggcg ctgaccgaca tcttcggggg gcggaaatac
     2521 aaggcgctgc tgcggcccgc gatcctcacg gcgttcctcg gctacctgtg ggtggccatc
     2581 gcgctgagct tcgacctcgg ccgctactgg aacatctggc gcccgatctt caactggcag
     2641 ggcaactcgg tcctgttcga agtcggcatg tgcgtcacgg tgtacctgtg cgttctcgca
     2701 atcgagatga gcccctccat cctggagggc ctcaaggcga ggatcgacga caacgagtgg
     2761 ggcgcggcgt tcctgcggcg gatcgagggg ccgatcatgg ccgcctaccg cggcgtgcgc
     2821 gtggcgctgc cgattgtcat cgtcgcgggc gtcgtgctct cctgcatgca tcagtcctcg
     2881 ctcggcacgc tgatgctcat cgcgccgacg aagatgagcc cgctctggga cacgagcgtg
     2941 ctgccgctgc tgttcctgtt ctcggcgttc atggtcggct tcccgatggt gatcctcgag
     3001 tcgatctacg cgaacatcag cttcggccga agcccggaga tggagctgct gacaccgctc
     3061 tcgcgcatca ttccgtggtt catcgggctg tacgcgctgc tgaaactcgg agacctggcc
     3121 gtgcgctggg acagcctgga cttcctggcg cggccgggcg ccacgacgtc gctggtggtg
     3181 gaaatcctgg tcggcgtcgt cgcgccgttc ctgctcctgc tcaacaaggc cgtccggcga
     3241 tcgatgggct ggctgttcct gtcggtggcc ctcatcatct tcggcgtcgt cctgaaccgg
     3301 atcaacgtgt tcctggtcgc ctacgaggcg ccgtattcga cgcggtcgta cttcccctcg
     3361 gtgggcgaga tcgcgatgac catcgcgatc gtgtcgagca tcctgttctg ctaccgcttc
     3421 ttcgtcacgt tctttcccat cctgcccggg ttccagccgc agcgcgtggc ggagctcgag
     3481 cgcctgcgcg aggagcgcgc gcgcacggtg agcccgttct ggacgtgggt gatccggggc
     3541 tcggcgatcg ccttcctgct ggcgttcgtg gcgatctaca acgtggtcca cgtgacggcg
     3601 gagcgaggca cggtgaagct cgtcgacggc accaacgtct acctcgccac cacgtcggg
//
LOCUS       DAOSIF010000311         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22331_length_3647_cov_3.292038, whole genome shotgun sequence.
ACCESSION   DAOSIF010000311 DAOSIF010000000
VERSION     DAOSIF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22331_length_3647_cov_3.292038"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLT35_10200"
     CDS             complement(<1..320)
                     /locus_tag="PLT35_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29636.1"
                     /translation="MQTKYMNPYLAGFFLGLVLLATIFITGRGLGASGAVKSVVVASV
                     AAAAPEHAARSAFYGPYVAPGAEHPLKAWLVFEVLGVMLGAYFSGLAAGRLRLVTEAG
                     PRVSR"
     gene            complement(332..964)
                     /locus_tag="PLT35_10205"
     CDS             complement(332..964)
                     /locus_tag="PLT35_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOG29637.1"
                     /translation="MRHYVYGGFERFWHWAQAVLILFLALTGFEIHGSIHFFGFEQAV
                     AYHSTAAYALMVLIAFAGFWHLTTGEWKQYVPTRENLLKQADYYLSGIFRGAPHPTKK
                     TPLSKLNPLQKLVYAGLKTFLIPLMVGSGLVYMFYRYPQRYSVVSLKVGGLEWVAIAH
                     TAGAFLLLAFVITHLYLITTGQTLTSNLKAMITGYEDLPDEDPHGPAASA"
     gene            complement(961..2628)
                     /locus_tag="PLT35_10210"
     CDS             complement(961..2628)
                     /locus_tag="PLT35_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HOG29638.1"
                     /translation="MRKILFGLLVLGVAAALIVGELHTKSTEQTPLEKLSRQYARRAG
                     PSVDHTKFAQLQARFKKPQEVTSACIACHNGRDEEVMRSSHWNWERTEYIPGQGIRSV
                     GKKNLVNNFCIGVTGNEPSCNRCHAGYGYAEASFDFTNPLNVDCLSCHDNSGEYTKAS
                     GGAGMPDPSVDLARVAQRVGKPQRLNCGTCHFLGGGGNNVKHGDLETALLDSPKTVDV
                     HMGTDGLDMECVDCHTASKHQMLGKMYSVSSMNRNRVECETCHSTLPHADGVLNSHTL
                     KVACQTCHIPVYAKVNPTKLTWDWSTAGRLQDGKPIEEKGPHGEDTYLSIKGSFTWGT
                     NVKPEYIWFNGTASHYLIGDRIDPSRPVKINTLHGSYDDPDAKIYPVKIHRGKQIYDA
                     EYRYLIQPKLVSTVPGDGGYWTEFDWPKANEEGMRLVGLPYSGKYGFVETEMTWPVNH
                     MVSPTSQTVGCNECHTRKNGRLAAVGGFYLPGRDRNGPVDAAGGGLLVATLAGVCLHG
                     AGRAVAARRKRRAMLVARPEPAPGAALDAQPEAAADAASETEGTERS"
     gene            complement(2796..>3647)
                     /locus_tag="PLT35_10215"
     CDS             complement(2796..>3647)
                     /locus_tag="PLT35_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29639.1"
                     /translation="QGGQPAKPLTPASQLQVPKMSFGGQQISRLIVGCNPFYGFSHFN
                     NTYSQVMREYYTAERVCEVLHQCARFGINTYNYVELGRAAEDLARFQAEGGSMHLIVQ
                     GIGDPAPLHAAWKPLAIYHHGGRTDTAYMSGKIETVRDWCKQVRDLGCLVGVGSHKPE
                     VLALVEDQGWDVDFYAGCVYNITRTNDEWRKVLGGELLEMPSEIYLQSDPPRMYRFMQ
                     QTKKPCFAYKILAAGRVGERAMDEAFRTAFASIKPTDGVFVGMFPRVRDEVRENAERV
                     CRILKTA"
BASE COUNT          516 a         1260 c         1250 g          621 t
ORIGIN      
        1 cgcgacacgc gggggccggc ttccgtcacc agcctcagcc ggccggccgc gaggccggag
       61 aagtacgcgc cgagcatgac gcccagcacc tcgaagacca gccacgcctt cagcgggtgc
      121 tcggcgcccg gcgcgacgta ggggccgtag aaggccgacc gcgcggcgtg ttccggggcc
      181 gccgcggcca ccgacgccac gacgacgctc ttcaccgccc cgctcgcgcc gaggccgcgc
      241 ccggtgatga agatcgttgc gagcagcacc aggccgagga agaacccggc caggtaggga
      301 ttcatgtact tcgtctgcac ggcaggttct cctacgccga ggcggcgggc ccgtgaggat
      361 cctcgtccgg caggtcctcg tagccggtga tcatcgcctt caggttcgac gtcagcgtct
      421 gccccgtcgt gatcaggtag aggtgcgtga tcacgaacgc cagcagcagg aacgcgcccg
      481 cggtgtgcgc gatcgccacc cactcgagcc cgcccacctt gaggctgacc accgagtagc
      541 gctgcgggta gcggtagaac atgtagacca gccccgagcc caccatgagc gggatcagga
      601 aggtcttgag gcccgcgtag accagcttct gcaacgggtt gagcttgctg agcggcgtct
      661 tcttcgtcgg gtgcggcgcg ccgcggaaga tgccggacag gtagtagtcc gcctgcttga
      721 gcaggttctc ccgcgtcggc acgtactgct tccactcgcc cgtcgtcagg tgccagaagc
      781 ccgcgaaggc gatgagcacc atgagggcgt acgccgccgt gctgtgatac gccaccgcct
      841 gctcgaagcc gaagaagtgg atcgagccgt gtatctcgaa ccccgtcagc gcgaggaaga
      901 ggatcaggac cgcctgggcc cagtgccaga agcgctcgaa cccgccgtac acgtagtgcc
      961 tcatgaccgc tccgtccctt ccgtctccga cgcggcgtcg gcggcagcct ccggctgagc
     1021 gtccagcgcc gcgccgggcg ccggctcagg ccgcgcgacc agcatggccc ggcgcttccg
     1081 ccgggcggca acggcgcgac cggcgccgtg gagacagacg ccggccaggg tggcgacgag
     1141 cagccccccg ccggcggcgt ccacgggccc gttgcggtcc cgtcccggca ggtagaagcc
     1201 gcccacggcc gcgagccggc cgttcttgcg cgtgtggcac tcgttgcagc cgacggtctg
     1261 cgatgtcggc gacaccatgt ggttcaccgg ccacgtcatc tcggtctcga cgaagccgta
     1321 cttgccgctg tacggcaggc cgaccagcct catgccctct tcgttggcct tcggccagtc
     1381 gaactcggtc cagtagccgc cgtcgccggg caccgtcgaa accagcttgg gctggatgag
     1441 gtagcggtac tcggcgtcgt agatctgctt cccgcggtgg atcttcaccg ggtagatctt
     1501 ggcgtccggg tcgtcgtagc tgccgtggag cgtgttgatc ttcaccggcc gcgacggatc
     1561 gatccgatcc ccgatcaggt agtgcgacgc cgtgccgttg aaccagatgt actcgggctt
     1621 gacgttcgtg ccccaggtga aggagccctt gatcgacagg taggtgtcct cgccgtgggg
     1681 gcccttctcc tcgatgggct tgccgtcctg cagccggccg gccgtggacc agtcccaggt
     1741 gagcttcgtc gggttcacct tcgcgtatac cgggatgtgg cacgtctggc aggccacctt
     1801 cagcgtgtgg ctgttcagga cgccgtcggc gtgcggcagc gtcgagtggc acgtctcgca
     1861 ctccacgcgg ttccggttca tcgacgacac cgagtacatc ttgccgagca tctggtgctt
     1921 cgacgccgtg tggcagtcca cgcactccat gtccaggccg tcggtcccca tgtggacgtc
     1981 caccgtcttc ggcgagtcga gcagggccgt ctccaggtcg ccgtgcttga cgttgttgcc
     2041 gccgccgccc aggaagtggc aggtgccgca gttcagccgc tggggcttcc cgacgcgctg
     2101 cgcgaccctg gccaggtcga ccgaggggtc cggcatccca gccccgcccg acgccttcgt
     2161 gtactcgccg ctgttgtcgt ggcagctcag gcagtccacg ttgagggggt tcgtgaagtc
     2221 gaacgacgcc tcggcgtagc cgtaccccgc gtggcagcgg ttgcagctcg gctcgttgcc
     2281 ggtcacgccg atgcagaagt tgttcaccag gttcttcttg ccgacgctcc ggatgccctg
     2341 gcccgggatg tactccgtgc gctcccagtt ccagtggctg gagcgcatca cttcctcgtc
     2401 gcggccgttg tggcaggcga tgcaggcgga ggtgacctcc tgcggctttt tgaaccgcgc
     2461 ctgcagctgc gcgaacttcg tgtggtccac cgacgggccc gcgcgccggg cgtactgccg
     2521 gctgagcttt tccagggggg tctgctccgt gctcttggtg tggagctcgc cgacgatgag
     2581 cgcggcggcc acgccgagaa ccaggagccc gaacaggatt tttctcatgt cgtggtgacc
     2641 tcaggcggcc cgggccgacg tgacggccgc cggctggccc gtgcctcgcg ccgggaccgc
     2701 ggggagcggc cgggcgccgc cggcgcggcg cccggtgcac tgcgtccgga cacagggatt
     2761 cacgcggccg ccgttgcgtg acgcgacgcg gccggtcagg cggtcttcag gatccggcac
     2821 acgcgctcgg cattctcgcg cacctcgtcc cggacgcgcg ggaacatccc gacgaagacg
     2881 ccgtcggtcg gcttgatgct cgcgaacgcc gtcctgaacg cctcgtccat ggcgcgctcg
     2941 ccgacccggc ccgccgcgag gatcttatag gcgaagcacg gcttcttcgt ctgctgcatg
     3001 aagcggtaca tgcggggcgg gtcgctctgc aggtagatct cggacggcat ctcgaggagt
     3061 tcgccgccga gcaccttccg ccactcgtcg ttcgtgcggg tgatgttata gacgcagccc
     3121 gcgtagaagt ccacgtccca gccctggtcc tcgacgagcg cgaggacctc cggcttgtgg
     3181 ctgccgacgc ccaccaggca cccaaggtcg cgcacctgct tgcaccagtc gcggaccgtc
     3241 tcgatcttcc cgctcatgta cgccgtgtcc gtgcgcccgc cgtggtggta gatggcgagg
     3301 ggcttccagg cggcgtggag gggcgccggg tcgccgatcc cctggacgat gaggtgcatc
     3361 gacccgccct cggcctggaa ccgggccagg tcctcggcgg cccgccccag ctcgacgtag
     3421 ttgtacgtgt tgatgccgaa ccgcgcgcac tggtgcagga cctcgcagac gcgctcggcc
     3481 gtgtagtact cccgcatcac ctgcgagtac gtgttgttga agtgcgagaa gccgtagaac
     3541 gggttgcagc cgacgatcag ccggctgatc tgctggccgc cgaagctcat tttcggcacc
     3601 tggagctggg acgccggggt gagcggcttg gccggctgtc cgccctg
//
LOCUS       DAOSIF010000312         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22359_length_3644_cov_3.532460, whole genome shotgun sequence.
ACCESSION   DAOSIF010000312 DAOSIF010000000
VERSION     DAOSIF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22359_length_3644_cov_3.532460"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3644
                     /locus_tag="PLT35_10220"
     CDS             <1..>3644
                     /locus_tag="PLT35_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29640.1"
                     /translation="TITTFAPRSIGSLAWTAAIKDVAIRRRKAGSTTTTTLFVTTGGP
                     AENLLAIDATSPGQLIVVGRASAAASQQRITLLPEASFTDRTGQVFSGDLAVSTHFNV
                     DSSSMVFWNVTNPAAPGVLSGKVLTLNPDFAGSVTTGAVLVNGFARGIAAVTTASGPV
                     AYATVDQVGVMAGVVKNNVPQRAPAQRLSEPVFGGQFTDVVAHQGMLYAVSPAAGNAF
                     AVLDPSLGLAGSTGVSFRSRRLRVVSGLQSDTNGDGLISSQEVFSLAVLAGDFGISVV
                     DVRDAGAPAEISRIPIPGVVRDLEVDTATRRIFAGVDLSSGGPALMVIDLSRPTLGAG
                     SDADGDGYDDRVLWTRSYGPGVNGLRLDADRGLLYIANPGGLDVWSVYDSCCDVGVDM
                     AAPIESQPLGTSDDLYAKELAAIRHGIIMGLDRARSACQRFDVTRLRMHESGSSACLW
                     GPDPAKNCGSNYQPGLSDHDISVFMPNDWYDEGALVPNPSKTGDPKDNRPSAITLAAC
                     TVDALSYPFTDVDDPDRRPREIDGFKFHDITFLPNFIEDFTSARYRLARTNPGEGDAD
                     NDLALGRQLLVMKHLTEAYGVKLQESDPGYKAEYANVGFTEADFTATMTKYRTETGIP
                     LVEGHEWSNLMEFLMVKARALVRIAGSEDETSAFHHEFIHQLHVAGKAGIRSALAAMV
                     ARPASRDLVLRFKRDETDPAAGLYILRGPGANACLEYEREIDGTLKAPALYSKARACG
                     SMEDYAASTAVRTLQVLDPADRPFTEDTVKEILRFYLVKSDEIQIRDAAEADRFLATV
                     YRFIQDVQAKTLDYWSLYTSGLIEREAIDQTPDPDPPFPQNQDRLWRRRDNMAKKVSR
                     LSEVGKVKLQVIPHVYNRSYRDVAGVDLVMYVAQPGQSASSLSCGTDENGASFCGIQI
                     DLEGGDHAYPDFRLRADGSFERTSSNGKKIPYFLLKVDQSAIPANTLGHVAFTLDLPK
                     RSLRESNRQNNLGGFYFYKLDTTGQTALPAIPATVPVPAAAGGSQALEGDAGCLASPA
                     LSVNQVMSIGGQVLQSPVALFMGETATLTLTVTNASSLDATGVMVCSTLTNECYPLGT
                     IAAGTSASASVPFSSSVPLSAQSIPTVYSNETSLQTGSPFNVTVAWTGFDVRSLEPDP
                     NPDQSTVMVGGTSYRHYAVVSTRTGRPAVGVPVVVAVTGARTGTATFTTDRNGVIGTP
                     VTGGDDEGRR"
BASE COUNT          595 a         1269 c         1260 g          520 t
ORIGIN      
        1 acgatcacca cgttcgcccc caggtcgatc ggatcgctgg cctggaccgc ggcgatcaag
       61 gacgtggcca tccgccgcag gaaggccggc tcgacgacga ccacgacgct gttcgtgacg
      121 accggcgggc cggcggagaa cctgctcgcc attgacgcca ccagccccgg ccagctgatc
      181 gtcgttgggc gcgcgtccgc ggccgcaagc cagcagcgga tcacgctgct gccggaggcg
      241 tcgttcacgg atcggaccgg gcaggtgttc agcggcgacc tggcggtcag cacgcacttc
      301 aacgtggact cctcgtcgat ggtgttctgg aacgtgacga accccgcggc gccgggcgtg
      361 ctgtcgggca aggtgctgac gctgaacccc gacttcgcgg ggagcgtcac gaccggcgcc
      421 gtgctggtga acggatttgc gcggggcatt gcggcggtga cgaccgcgtc ggggcccgtg
      481 gcctatgcca ccgtcgacca ggtcggcgtg atggcgggcg tcgtcaagaa caacgtgccc
      541 cagcgggcgc cggcgcagcg cctctcggag cccgtgttcg gcggccagtt cacggacgtc
      601 gtggcccacc agggcatgct ctacgcggtg agcccggccg ccggaaacgc ctttgcggtg
      661 ctcgacccga gcctcgggct cgccggcagc acgggcgtgt cgttccggtc gcgccgcctc
      721 cgcgtcgtga gcggcctgca gtcggacacg aacggcgacg ggctcatctc gtcgcaggag
      781 gtgttctcgc tcgccgtgct ggccggggac ttcgggatct ccgtcgtgga cgtgcgggac
      841 gccggggcgc cggccgagat cagccgcatt ccgatcccgg gcgtcgtgcg cgacctcgag
      901 gtcgacacgg ccacccggcg catcttcgcc ggagtggacc tgtcaagcgg cgggccggcg
      961 ctgatggtca tcgacctgtc cagacccacg ctcggagccg gctcggacgc cgacggcgac
     1021 gggtacgacg acagggtgct gtggacgcgg agctacgggc cgggcgtcaa cggcctgcgc
     1081 ctcgacgccg atcgcggctt gctctacatc gccaatccgg gcggcctcga cgtctggagc
     1141 gtctacgaca gttgctgcga cgtcggcgtg gacatggcgg cccccatcga gtcgcagccg
     1201 ctgggcacca gcgacgacct ctacgcgaag gagctggcgg ccatccggca cggcatcatc
     1261 atgggcctcg accgggcccg gagcgcatgc cagcggttcg acgtcacccg gctcaggatg
     1321 cacgagtcgg gcagcagcgc ctgcctctgg gggcccgacc cggcaaagaa ctgcggctcg
     1381 aactaccagc ccggcctgag cgaccacgac atctcggtgt tcatgccgaa cgactggtac
     1441 gacgagggcg cgctcgtgcc gaatcccagc aagacgggcg acccgaagga taacaggcct
     1501 tcggcgatca cgctcgcggc gtgcacggtc gacgcgctca gctatccctt cacggacgtg
     1561 gacgacccgg acaggcggcc cagggagatc gacgggttca agttccacga catcacgttc
     1621 ctgccgaact tcatcgagga cttcacgtcg gcccgctacc ggctcgcgcg cacgaacccg
     1681 ggcgagggcg acgcggacaa cgatctcgcg ctggggcggc agctgctggt gatgaagcac
     1741 ctcaccgagg cgtacggggt gaagctgcag gagagcgacc ccgggtacaa ggccgagtac
     1801 gcgaacgtcg gcttcaccga ggcggacttc acggccacga tgacgaagta ccgcaccgag
     1861 acgggaatcc cgctggtcga gggccacgag tggtcgaacc tgatggagtt cctgatggtg
     1921 aaggccaggg ccctcgtgcg gattgccggc tccgaggacg agacgtcggc cttccaccac
     1981 gagttcatcc accagctgca cgtcgcgggc aaggcgggca tccggtcggc gctcgcggcg
     2041 atggtcgcgc gcccggcctc gcgcgatctg gtcctgcgtt tcaagcgcga cgaaacggac
     2101 ccggccgcgg gcctctacat cctgcgaggc ccgggtgcga acgcctgcct cgagtacgag
     2161 cgtgagatcg acggcacgct gaaggccccg gcgctctact cgaaggcccg cgcatgcggc
     2221 tcgatggagg actacgccgc gtcgacggcc gtgcgcacgc tgcaggtgct cgatcccgcc
     2281 gaccggccgt tcacggagga caccgtcaag gagatcctcc gcttctacct cgtgaagtcg
     2341 gacgagatcc agatccgcga cgccgccgag gccgaccggt tcctcgcgac ggtctaccgg
     2401 ttcatccagg acgtgcaggc gaagaccctc gactactgga gcctgtacac gagcggcctg
     2461 atcgaacggg aggcgatcga ccagacgccc gacccggacc cgccgttccc gcagaaccag
     2521 gaccggctct ggcgccgccg cgacaacatg gcgaagaagg tcagccggct gtccgaggtc
     2581 ggcaaggtca agctccaggt gatcccgcac gtctacaacc ggagctaccg cgacgtcgcc
     2641 ggcgtcgacc tggtgatgta cgtggctcag cccgggcaga gcgcgtcgag cctctcctgc
     2701 gggaccgacg agaacggcgc ctcgttctgc ggcatccaga tcgacctcga gggcggcgac
     2761 cacgcctatc cggacttccg cctccgggcc gacggctcgt tcgagcggac aagctcgaac
     2821 ggcaagaaga tcccgtactt cctcctgaag gtggaccagt ccgcgattcc cgcgaacacg
     2881 ctgggccacg tcgcgttcac cctcgacttg ccaaagcggt ccctgcgcga gtcgaaccgc
     2941 cagaacaacc tcggcggctt ctacttctac aagctcgaca cgacggggca gacggcgctg
     3001 ccggccatcc cggcgaccgt gccggtgccc gctgcggcag gcggcagcca ggccctcgag
     3061 ggcgacgcgg gttgcctggc gagccccgcg ctgtcggtca accaggtcat gtccatcggc
     3121 ggccaggtcc tgcagagccc tgttgcgctg ttcatgggcg agacggcgac cctgacgctg
     3181 accgtcacga acgcgtcctc cctcgacgca acgggcgtga tggtctgcag cacgctgacg
     3241 aacgagtgct atcccctggg caccatcgcg gcgggcacga gcgcgtcggc gtcggtgccg
     3301 ttctcgtcca gcgtgccgct cagcgcgcag tcgatcccga cggtgtattc gaacgagacc
     3361 tcgctgcaga ccggaagccc cttcaacgtg acggtggcct ggaccgggtt cgacgtgaga
     3421 tccctcgagc cggatcccaa cccggatcaa tcgaccgtca tggtcggggg cacctcctac
     3481 cgccactacg ccgtcgtgag cacgcggacg ggccgtccgg cggtcggagt gccggtcgtc
     3541 gtggccgtta cgggcgcgag gacaggcacg gcgaccttca ccaccgaccg gaacggcgtc
     3601 atcgggacgc ctgtgaccgg cggcgacgac gagggccgga ggtt
//
LOCUS       DAOSIF010000313         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22379_length_3641_cov_4.142220, whole genome shotgun sequence.
ACCESSION   DAOSIF010000313 DAOSIF010000000
VERSION     DAOSIF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22379_length_3641_cov_4.142220"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLT35_10225"
     CDS             <1..288
                     /locus_tag="PLT35_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29641.1"
                     /translation="PAIMWPSVAYVVEETRLGTAYGLMTLVQNIGLAGFNLVIGWAND
                     AAGASAANPAGYRPGMWIFSTLGVSAVLCSLLLWRREHGPHAHGLETIRAA"
     gene            346..1626
                     /locus_tag="PLT35_10230"
     CDS             346..1626
                     /locus_tag="PLT35_10230"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG29642.1"
                     /translation="MIRSKVAILRTRPSTVLRDYHELMNLAGYQDVIARDADTALKVN
                     ISWHFFFPGSSTVPWQLDGVIRALKADGYDPNLVHACHNRTVVIDAHLGERENKQIDV
                     VRAHGLRNIHLYEGEEWMDIRDAVGDLAKQFLCLNEVYPKGFLIPKRFIGENIIHLPT
                     VKTHVFTTMTGAMKNAFGGLLNERRHWTHPVIHETLVDLLRIQQRIHRGVFAVTDGTF
                     AGDGPGPRCMVPHVKNVILASADQVAIDAVAAKLMGMDPLSIAFIRLAHDAGLGCGDP
                     REIEIVGDQEAAKDNWRFAGPFKKMTFASRMQHRIYWGPLKKPLEWSLKTVLAPWSYI
                     ASVVYHDSYWYPRHADRIMANVLGSEWGRLFQNWTDAAAKADENGYPDVGEAPADLTR
                     LGAKAFARSLRLLGMAVTEAPEFANRKRKIAAAP"
     gene            1679..2407
                     /locus_tag="PLT35_10235"
     CDS             1679..2407
                     /locus_tag="PLT35_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG29643.1"
                     /translation="MIDVQRLTKTYGDLRAVDDLSFHVAPGEIVGLIGPNGAGKTTTL
                     RCMVGIQAPSAGTITIGGHDIVASPVEAKRLLAFMADEPPLFDYLTVMEHLRLTARLY
                     RVRDAEARARALLAELQLTGKEHALPAELSRGMKQKAAIACGLLHDPAALLFDEPLTG
                     LDPLGIRHMKETIRARGRAGAAVIVSSHLLHLVEEICTRIVIIDRGAKVADATLDELR
                     ARPELAAAGSSLEQIFITATAGEA"
     gene            2408..>3641
                     /locus_tag="PLT35_10240"
     CDS             2408..>3641
                     /locus_tag="PLT35_10240"
                     /inference="COORDINATES: protein motif:HMM:NF028272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HOG29644.1"
                     /translation="MLGAFLYLTACSVKNRTARRVRLLRKPRYAAGLVVGLLYMWFAF
                     LRRAGGRAAADADAAQAALEVVAGPLQFAGSLLLLAAAAAAWVWPGAGQAFAFSRAEV
                     QFLFPAPVTRRQLVHYELLRSQLAILFGSVIATLFMRGGARAGSWAFLSGLWVLLMVV
                     RLHLAGVALRRTSLVQHGASGWARHWLPLAAVAGALAVFGAGIAGRWPALAGAATGGE
                     VFRELQRLGSEGATGILLWPFRALVRLPLSASAADFWRALPPALAILAANYVWVLRSD
                     AAFEEASAAHAEKRASDRRAPRAAVKGAAPTPFTLAAAGPPETAVLWKNLILVGRYAS
                     IRTLVRLVPIVLVLGVVAQGGGGGIAATAATACLAFAAFAVLMGTQMVRNDLRQDLPH
                     LALLKTWPLRGAALLRGEA"
BASE COUNT          514 a         1300 c         1295 g          532 t
ORIGIN      
        1 cccgccatca tgtggccgtc ggtggcctac gtcgtcgagg agacgcgcct cgggaccgcg
       61 tacggcctga tgaccctcgt ccagaacatc ggcctggccg ggttcaacct cgtgattggc
      121 tgggcgaacg acgccgcggg cgcgagcgcg gcgaaccccg ccggctaccg cccgggcatg
      181 tggatcttct cgaccctcgg cgtctcggca gtcctgtgct cgctgctcct ctggcggcgc
      241 gagcacgggc cgcacgcgca cggcctcgaa acgattaggg ccgcctgagc ctgcgcggcc
      301 ccggcagccc ccgtcctggc gtaagatggc gggagaggca ccgccatgat ccgatccaag
      361 gtcgcgatcc tccggacgcg gccgtccacc gtgctgcgcg actaccacga actgatgaac
      421 ctggccggct accaggacgt catcgcgcgg gacgcggaca ccgcgctgaa ggtcaacatc
      481 tcctggcact tcttcttccc gggcagttcc accgtgccct ggcagctcga cggcgtgatc
      541 cgcgcgctga aggcggacgg gtacgacccg aacctcgtcc acgcctgcca caaccgcacg
      601 gtggtcatcg acgcgcacct cggcgagcgc gagaacaagc agatcgacgt cgtccgggcc
      661 cacgggctgc ggaacatcca cctctacgaa ggcgaggaat ggatggacat cagggacgcc
      721 gtcggcgacc tggcgaagca gttcctctgc ctgaacgagg tctacccgaa gggcttcctc
      781 atcccgaagc ggttcatcgg cgagaacatc atccacctgc cgaccgtgaa gacgcacgtc
      841 ttcacgacga tgaccggcgc aatgaagaac gcgttcggcg ggctgctcaa cgagcggcgc
      901 cactggacgc acccggtgat ccacgaaacg ctcgtggacc tgctgcgcat ccagcagcgg
      961 atccaccgcg gggtgttcgc cgtgaccgac ggcaccttcg ccggcgacgg cccggggccc
     1021 cggtgcatgg tgccgcacgt caagaacgtc atcctcgcga gcgccgacca ggtggccatc
     1081 gatgccgtcg ccgcgaagct gatgggcatg gacccgctgt cgatcgcgtt catccgcctc
     1141 gcccacgacg ccgggctggg ctgcggggat ccgcgcgaga tcgaaatcgt cggcgaccag
     1201 gaggccgcga aggacaactg gcgcttcgcc gggccgttca agaagatgac cttcgcgtcg
     1261 cgcatgcagc accggatcta ctgggggccc ctcaagaagc ccctcgagtg gagcctgaag
     1321 accgtgctcg ccccctggtc ctacatcgcc agcgtcgtct accacgacag ctactggtac
     1381 ccgcgccacg ccgaccggat catggcgaat gtgctcggga gcgagtgggg ccgcctgttc
     1441 cagaactgga cggacgccgc ggccaaggcc gacgagaacg ggtacccgga cgtcggcgag
     1501 gcgccggccg acctcacccg gctgggcgcg aaggccttcg cgcgatcgct ccggctgctg
     1561 ggcatggccg tgaccgaggc acccgagttc gcgaaccgga agcggaagat cgccgcggcg
     1621 ccgtaacccc gggggcggca ggatttcgga atccggaatc cagaagtcag gaatcggcat
     1681 gatcgacgtg cagcgcctga cgaagacgta cggcgacctc cgcgcggtcg acgatctctc
     1741 cttccatgtc gcgcccgggg agatcgtcgg cctcatcggc ccgaacggcg ccggcaagac
     1801 gaccacgctc cgctgcatgg tcggcatcca ggcgccgtcg gcggggacca tcactatcgg
     1861 cggccacgac attgtcgcgt cgcccgtcga ggcgaagcgg ctgctggcgt tcatggccga
     1921 tgagccgccg ctcttcgact acctcacggt gatggagcac ctgcggctga ccgcgcggct
     1981 ctaccgcgtg cgcgacgccg aggcgcgggc cagggcgctc ctcgcggaac tgcagctgac
     2041 gggcaaggag cacgcgctgc cggccgagct gtcgcggggg atgaagcaga aggcggccat
     2101 cgcgtgcggg ctgctgcacg atccggccgc gctgctcttc gacgagcccc tcacggggct
     2161 cgatcccctc ggcatccggc acatgaagga gaccatcagg gcgcgcgggc gggccggcgc
     2221 ggccgtcatc gtctcctcgc acctgctcca cctcgtcgag gagatctgca cgcgcatcgt
     2281 gatcatcgat cgcggcgcga aggtcgccga cgcgaccctc gatgagctgc gcgcgcgccc
     2341 cgaactcgcg gcggccggtt caagcctgga gcagatcttc atcaccgcga ctgccggcga
     2401 ggcctaggtg ctcggcgcct tcctctacct cacggcgtgc tcggtgaaga accggaccgc
     2461 acggcgcgtg cgcctgctgc ggaagccgcg gtacgcggcc ggcctggtcg tcggcctcct
     2521 ctacatgtgg ttcgcgttcc tgaggcgcgc gggcggacgg gcggcggcgg acgccgacgc
     2581 ggcgcaggcg gccctcgagg tcgtggcggg gcccctccag ttcgccggga gcctcctgct
     2641 cctcgcggcg gccgccgcgg cgtgggtctg gcccggcgcc ggacaggcgt tcgcgttctc
     2701 gcgggcggaa gtgcagttcc tgttcccggc cccggtcacg cggcgccagc tcgtgcacta
     2761 cgagctgctg cggtcgcagc tggccatcct cttcggcagc gtgatcgcca cgctgttcat
     2821 gcgcggcggg gcgcgggccg ggtcctgggc gttcttgtcg gggctgtggg tgctcctcat
     2881 ggtggtgcgg ctgcacctgg caggcgtcgc cctgaggcgg accagcctcg tccagcacgg
     2941 cgcgagcggg tgggcgcgcc actggcttcc gctggccgcc gtggccggcg cgctggccgt
     3001 gttcggcgcc ggcatcgccg ggcgctggcc ggcccttgcc ggcgccgcaa cgggcgggga
     3061 ggtgttccgc gagttgcagc gcctgggcag cgaaggcgcg acgggcatcc tgctctggcc
     3121 gttccgcgcg ctggtgcggc tcccgctgtc ggcgtcggcg gccgacttct ggcgggcgct
     3181 gccgccggcc ctggccatcc tagccgcgaa ctacgtctgg gtgctcaggt ccgacgcggc
     3241 gttcgaggaa gcctcggcgg cccacgccga gaagcgcgcg tcggaccgcc gggcgccccg
     3301 ggcggctgtc aaaggcgcgg ctccgacgcc gttcacgctg gcggcagcag gcccgcccga
     3361 gacggcggtg ctctggaaga acctgatcct ggtgggccgc tacgcgtcca tccggacgct
     3421 ggtgcggctt gtgccgatcg tcctggtgct cggcgtcgtt gcgcagggcg gcggcggcgg
     3481 gatcgcggcc acggccgcca ccgcctgcct ggcgttcgcg gccttcgcgg tgctgatggg
     3541 aacgcagatg gtgcggaacg atctgcgcca ggatctccct cacctcgcgc tgctcaagac
     3601 gtggcccctg cgcggcgccg cgctcctgcg cggagaggcg c
//
LOCUS       DAOSIF010000314         3639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22393_length_3639_cov_3.296596, whole genome shotgun sequence.
ACCESSION   DAOSIF010000314 DAOSIF010000000
VERSION     DAOSIF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22393_length_3639_cov_3.296596"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLT35_10245"
     CDS             <1..253
                     /locus_tag="PLT35_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG29645.1"
                     /translation="GLVRDQIKIELQHDTLTVRGDRPVAREEGAQFHRVERGHGPFSR
                     SFPLPQPVDADAIAAEFRDGVLTIVVPKAAPASRRIQVD"
     gene            255..1439
                     /locus_tag="PLT35_10250"
     CDS             255..1439
                     /locus_tag="PLT35_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOG29646.1"
                     /translation="MKRLAFAVVAILMGFFAGLVMTGRLHRAPAGEVAAAAQPGGGDQ
                     AAAPAGAGARAAAGLPDFTAVADRAVGAVTNISSAQVVRSSNSPFANDPFFRYFFGDD
                     MDLFGGRDRREASLGSGVVVSSDGYILTNNHVVGSDTAEITVALADKRELRADLVGTD
                     PYTDIALLKVRATGLPTIPWGDSSRLKVAEWVLAIGNPFQLNQTVTLGIVSAVGRANV
                     GVAAYEDFIQTDAAINPGNSGGALINARGELVGINTAIYSQSGGYQGVGFAVPSNLAR
                     RVMTDLVRYGEVRRGSLGYLQVREMNESLAQQLGLPAAKGVLVERMARDSAAYDAGIR
                     PGDVIVSFNRKSIEDSGQLLRLIADSPIGSSVTVGAYRDGRMIELKAQVGAMRQRTTA
                     RR"
     gene            complement(1390..2088)
                     /locus_tag="PLT35_10255"
     CDS             complement(1390..2088)
                     /locus_tag="PLT35_10255"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOG29647.1"
                     /translation="MSGRHPGGPRPLPRTFYERPTLEVARDLLGKVLVRRDAEGLASG
                     LIVETEAYIGESDPACHAASGPTPRNQPLYGAPGRAYVYFTYGMHYLVNAVTERRGFP
                     AAVLIRALEPIEGVDLMRRRRVGRGPGGARRRGSARALPAHHLCRGPGNLARALGITL
                     AENRADLTKRAGRNGAGLWIEDRGVPVDDVVWTPRIGIRSGTDLAWRCVAAGSPAAST
                     FSPSSAAASRRPGP"
     gene            complement(2085..3425)
                     /locus_tag="PLT35_10260"
     CDS             complement(2085..3425)
                     /locus_tag="PLT35_10260"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG29648.1"
                     /translation="MSERVLAGIVAGLLLWLPAGAAAAAAPPQGRQVAFEQVVESLKS
                     PDAKARLGAIQLLREAGYLEAAPAIAPLLADPDDRVRAAAIEGLVSMYLVDERHTREF
                     GRGLIREKGATLPLYAFVQREGVTIPNLAPPEVIRGLIGAAASPNLRIRFDAAYALAV
                     LGRPLVRQGQFPDGRAAVNALMAILREPDPLMRLAATHALGRLMGAARQSPGASPELT
                     AQRTEVGDQIVVGMNDPDEDVRLASMGALGEMRYERGVQSLTDLYNYYKKGVEGMTAL
                     DALAKIAHPGSLPVFLAQLGHREAHIRRLAIEGIGRTGDEAAMREMESRTGRDQSPYV
                     TYARAFARALNRDYSQLPKLVEGFKYSLLESDTFDYLVELGPPAAAELAALAANKDQK
                     VRAGIAEVLGITGSQASLETLAALAADRADLVAAAAERSQKRLVPRDRAAPRAR"
     gene            complement(3422..>3639)
                     /locus_tag="PLT35_10265"
     CDS             complement(3422..>3639)
                     /locus_tag="PLT35_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29649.1"
                     /translation="HSLLVIEHNLDVIKTADYVIDLGPEGGDGGGRVVAVGTPEEVAS
                     VPASHTGRFLRAVLTANRRAEELGALA"
BASE COUNT          493 a         1354 c         1292 g          500 t
ORIGIN      
        1 cggcctggtg cgggaccaga tcaagatcga gttgcagcac gacacgctca ccgtccgcgg
       61 cgaccggccg gtcgcgcggg aggagggcgc ccagttccac cgcgtcgagc gcggccacgg
      121 tccgttttcc cgctcgttcc cgctgccgca gcccgtcgat gccgacgcca tcgcggccga
      181 gttccgcgac ggcgtgctga cgatcgtggt cccgaaggcg gccccggcct cgcgccgcat
      241 ccaggtggac tgatatgaag cgcctcgcct tcgccgtcgt cgccatcctc atgggcttct
      301 tcgcaggcct ggtcatgacc ggccggctgc atcgcgcgcc ggcgggcgag gtcgcggcgg
      361 ccgcgcaacc cggcggcggc gatcaggccg ccgccccggc cggcgccggc gcccgcgccg
      421 ctgccggcct gcccgacttc acggccgtcg ccgaccgcgc cgtcggcgcg gtgacgaaca
      481 tctcctcggc gcaggtggtc cgctcgtcga actcgccgtt cgccaacgat ccgttcttcc
      541 gctacttctt cggcgacgac atggacctgt tcggcgggcg cgaccggcgc gaggccagcc
      601 tcgggtcggg cgtggtcgtg tcgagcgacg gctacatcct caccaacaac cacgtcgtcg
      661 gcagcgacac cgcggagatc acggtggcgc tcgcggacaa gcgcgaactg cgcgccgacc
      721 tggtcggcac ggatccctac accgacatcg cgctgctgaa ggtccgcgcg acggggctgc
      781 cgaccatccc gtggggcgat tcgtccaggc tcaaagtggc cgagtgggtc ctcgccatcg
      841 gcaacccgtt ccagctcaat cagaccgtca ccctcggcat cgtgtccgcc gtcgggcggg
      901 ccaacgtcgg cgtggcggcc tacgaggact tcatccagac ggacgccgcc atcaacccgg
      961 ggaactcggg aggcgccctg atcaacgcgc gcggcgagct cgtcggcatc aatacggcca
     1021 tctacagcca gagcggcggc taccagggcg tggggttcgc cgtgcccagc aacctcgcgc
     1081 ggcgggtgat gaccgatctc gtcaggtacg gcgaggtgcg gcgcggctcg ctcggatacc
     1141 tccaggtgcg cgagatgaac gagtcgctcg cgcagcaact gggcctcccg gcggccaagg
     1201 gtgtcctcgt cgagcgcatg gcgcgcgact cggcggccta cgacgccggc atccgccccg
     1261 gcgacgtgat cgtgtccttc aaccggaaga gcatcgaaga cagcggccag ctgctgaggc
     1321 tgattgccga ctcccccatc ggcagcagcg tgaccgtggg cgcctaccgc gacggccgca
     1381 tgatcgagct caaggcccag gtcggcgcga tgcggcagcg gacgacggcg agaaggtaga
     1441 cgccgcgggg ctcccggccg cgacgcaccg ccacgcgaga tccgtccccg agcggatgcc
     1501 gatgcgcggc gtccacacga cgtcgtccac cggcacgccg cgatcctcga tccagagccc
     1561 cgccccgttt cgacccgccc gcttcgtgag atccgcgcgg ttctcggcga gggtgatccc
     1621 gagggccctg gcgaggttgc cggggccgcg gcacaggtga tgggccggca gtgcgcgggc
     1681 gctgccccgg cgccgcgccc cgccgggccc gcggccgacg cggcggcggc gcatcaggtc
     1741 tacgccctcg atcggctcga gggcccggat gagcacggcg gccgggaagc cgcgccgctc
     1801 ggtcacggcg ttcaccaggt agtgcatgcc gtaggtgaaa tagacgtagg cgcggccagg
     1861 cgcgccgtag agcggctggt tgcgcggcgt ggggccggac gcggcgtggc aggcggggtc
     1921 cgactcgccg atgtacgctt ccgtttcgac gatcaggccc gacgccagcc cctcggcgtc
     1981 ccggcgcacg agcaccttgc ccagcaggtc gcgggccacc tcgagcgtgg ggcgctcgta
     2041 gaacgtccgc ggcagcgggc gcgggccgcc gggatgcctc ccgctcatcg cgcccgcggc
     2101 gccgccctgt cccggggcac cagccgcttc tgcgatcgct cggcggccgc cgccaccagg
     2161 tcggcgcgat ccgccgccag tgccgcgagc gtctcgagcg acgcctgcga cccggtgatg
     2221 ccgagcacct ccgcgatgcc cgcgcgcacc ttctggtcct tgtttgccgc cagcgcggcc
     2281 agctccgcgg ccgccggcgg cccgagttcg acgaggtagt cgaacgtgtc cgactcgagc
     2341 agcgagtact tgaacccctc gacgagcttg ggcaactggc tgtagtcccg gttcagggcg
     2401 cgggcgaacg cccgcgcgta cgtcacgtac ggcgactggt cccgccccgt ccgcgactcc
     2461 atctccctca tcgcggcctc gtcgcccgtc cggccgatgc cctcgatggc gagccggcgg
     2521 atatgcgcct cccggtgccc gagctgggcc aggaacacgg gcaggctgcc cggatgcgcg
     2581 atcttggcca gcgcgtcgag agccgtcatc ccctcgacgc ctttcttgta atagttgtac
     2641 aggtcggtga gcgactgcac ccctcgctcg taccgcatct cgcccagcgc gcccatcgac
     2701 gccaggcgca cgtcctcgtc cgggtcgttc atgccgacca cgatctggtc gcccacctcg
     2761 gtgcgctgag ccgtcaactc ggggctggcg ccggggctct ggcgggcggc gcccatcagg
     2821 cgcccgagcg cgtgcgtggc ggccaggcgc atcagggggt cgggctcgcg caggatggcc
     2881 atcagggcgt tgaccgcggc ccgtccgtcg ggaaactggc cctgcctgac gagaggccgc
     2941 ccgagcacgg ccagcgcgta cgcagcatcg aagcgaatgc ggaggttggg cgaggccgcc
     3001 gcgccgatga ggccccggat gacttccggc ggggcgaggt tggggatggt gaccccttcg
     3061 cgctggacga aggcgtaaag cggcagggtg gcgcccttct cgcggatgag gccgcgcccg
     3121 aactcgcgcg tgtgccgctc gtccaccagg tacatggaca cgagcccctc gatggcggcg
     3181 gcgcgcaccc ggtcgtcggg gtccgccagg agcggcgcga tcgcgggcgc cgcctcgagg
     3241 taccccgctt cgcgcagcaa ctggattgcg cccagccgcg ccttcgcgtc gggactcttg
     3301 agcgactcga cgacctgctc gaaggcgacc tgccggcctt gcggcggcgc ggcagcggcc
     3361 gcggcgcccg ccggcagcca cagaaggagg ccggcgacga ttccggcgag aacccgctcg
     3421 ctcatgccag tgctcccagt tcctcggcgc ggcggttcgc cgtcagcacc gcccgcagga
     3481 aacgcccggt gtgcgaagcg ggcaccgaag cgacttcctc cggcgtgccg acggccacga
     3541 cccgcccgcc accgtctccg ccctcggggc cgaggtcgat cacgtagtcc gcggtcttga
     3601 tcacgtccag gttgtgctcg atgacgagca gcgagtgcc
//
LOCUS       DAOSIF010000315         3610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22614_length_3610_cov_2.990436, whole genome shotgun sequence.
ACCESSION   DAOSIF010000315 DAOSIF010000000
VERSION     DAOSIF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22614_length_3610_cov_2.990436"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1142
                     /gene="hprK"
                     /locus_tag="PLT35_10270"
     CDS             141..1142
                     /gene="hprK"
                     /locus_tag="PLT35_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOG29650.1"
                     /translation="MSPSTPRVTVRSLLLVQSDALNLGLEVLAGAEGLDRAISSPYVL
                     KTGLALAGFDAYLQAGRVLIFGASELGYLVGLEAGARRESLARAFGHDIPCVLVTLGL
                     EVPPEVLAEADRTGVPVLRTPLATPVAIAKLTSFLEDTLAERTIVHAVLMDILGLGVL
                     IVGESGIGKSECALDLVLRGHRLVADDTVEIRRVSESVLIGTCPEPTRYHMELRGLGI
                     INVLDIFGVASTRPSKRLELVVQLERWEAGREYERLGLDDRFYEILGLRVPMNRMPVA
                     PGRSVANLVEVAARTQLMRARGRHAARELADQLGDRLRRARLRDAAEPPDPDGRGSA"
     gene            1142..2101
                     /gene="rapZ"
                     /locus_tag="PLT35_10275"
     CDS             1142..2101
                     /gene="rapZ"
                     /locus_tag="PLT35_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOG29651.1"
                     /translation="MATRTKDRRGSRRAVQARLVIVTGLSGAGKSHAIRALEDLGYFC
                     VDNLPTLLIPTMAGFATRAGSEIEKVAIVVDVREGAFLSDFARVYRSVRRMPGLDAVL
                     IFLEASDAALLRRFSETRRPHPLAHDRPVLDGIRDERRRLARIRAMADEIVDTSSLTV
                     YDLRDVFAAHSRGGGAPGRPLQATIVSFGFKHGIPVEADLLFDVRFLPNPFFQPRLRP
                     LTGRDRRVAAFMRAHPLTQETIRRIASLLQFLVPHYADEGKSYLTIGIGCTGGQHRSV
                     FIAEALRRALGPVGGVRFHVRHRDLASAGEPGAAGRRRQGAQT"
     gene            2098..2508
                     /locus_tag="PLT35_10280"
     CDS             2098..2508
                     /locus_tag="PLT35_10280"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG29652.1"
                     /translation="MIGVVVVTHGQLATELLNAAEAIVGDLPRFAAVSIGWHDDTDDA
                     REEIRQAIARLQGEHGVLVLTDMFGGTPTNLGLSFVEPDRVEVVTGVNLPMLIKLASL
                     RASQDLLAVARQLRETGRNAIHVASDLLRTEPKG"
     gene            2510..2791
                     /locus_tag="PLT35_10285"
     CDS             2510..2791
                     /locus_tag="PLT35_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOG29653.1"
                     /translation="MRSRTVTIANALGLHARAAARFVHAASAFQCRVTVVRTDRAERT
                     VDGKSILGLLLLAAAKGAAVRITADGPDEAEALDALCRFVESGFGEGAC"
     gene            2785..>3610
                     /locus_tag="PLT35_10290"
     CDS             2785..>3610
                     /locus_tag="PLT35_10290"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOG29654.1"
                     /translation="MLTLAGLGVSPGVAWGPAALLRQRQPAVRYLIAPPRVAMETGRL
                     EAAREATARQLRAVLAKVAGSAVAEFSHLFEAQLLMLDDAMLVARAKSIIARERINAE
                     WALDRAFDEFSAIFAHVEDAYLRERRGDVADVVGRLRHNLLAAHPGDADFLPDLAAPA
                     ILVAADLAPSFAAQIDRRTILGLALETGSRTHHSAILARSLGLPAVAGAAGVTRHILP
                     GTVVLVDGDRGTVVIDPTPEALDEAAARAERLRTRAASARAAERGPALTADGVAILF"
BASE COUNT          471 a         1323 c         1312 g          504 t
ORIGIN      
        1 cggatcatcg gcccgaaccc ggacgcgttc gtcgtgttcc ggaacgccga ctccgacgcg
       61 ctgagcgtca tgttccggcg caaggacggc gacatcgggc tgatcgagcc ggaaccgtga
      121 ccgtcgggcc gccggggcgc atgtcaccgt cgacgccgcg cgtcaccgtc agatcgctgc
      181 tcctcgtgca gtcggacgcc ctgaatctcg gccttgaagt gctcgccggg gcggagggcc
      241 tcgatcgggc gatctccagc ccgtacgtcc tgaagaccgg gctggcgcta gcggggttcg
      301 acgcgtactt gcaggccggg cgcgtcctca tcttcggggc cagcgagctc ggctatctcg
      361 tcgggctcga ggctggagcc cgtcgcgagt ctctcgcccg cgcgttcgga cacgacatcc
      421 cgtgcgtcct cgtgacgctc ggcctcgagg tgccgcccga ggtcctggcc gaggccgacc
      481 gcaccggcgt gccggtgctg cgcaccccgc tggctacgcc ggtggccatc gcgaagctga
      541 ccagcttcct cgaggacacg ctggccgagc gcacgatcgt ccacgccgtg ctgatggaca
      601 tcctcggcct cggcgtcttg attgtcggcg agagcggcat tggcaagagc gagtgcgcgc
      661 tcgacctggt gctgcgcggc caccgcctcg tggccgacga cactgtcgag atccggcgcg
      721 tgtcggagtc ggtgctgatc ggcacctgcc cggagccgac gcgctaccac atggagctgc
      781 ggggcctggg gatcatcaac gtcctcgaca tcttcggcgt ggcgtccacg cggccgtcca
      841 agcggctcga gctcgtcgtc cagctcgagc gctgggaggc cggccgcgag tacgagcggc
      901 tcgggctcga cgaccggttc tacgagatcc tcggcctccg ggtgccgatg aaccgcatgc
      961 cggtcgcccc gggccgcagc gtggccaacc tcgtggaggt cgcggcccgc acccagctga
     1021 tgcgcgcgcg ggggcggcac gccgcgcgcg agctggcgga tcagctcggc gatcggttgc
     1081 ggcgcgcgcg cctgcgggac gcggccgagc cgcccgaccc ggacggccgg gggagcgcct
     1141 gatggcgacg cgcacgaagg atcggcgggg gagccgccgc gcggtccagg cccggctggt
     1201 gatcgtcacg ggcctctccg gggccgggaa gtcgcacgcc attcgcgccc tcgaggatct
     1261 cgggtacttc tgcgtggaca acctgccgac gctcctcatc ccgaccatgg cggggtttgc
     1321 cacccgcgcg ggctccgaga tcgagaaagt cgcgattgtc gtcgacgtgc gcgagggcgc
     1381 gttcctctcg gacttcgccc gggtgtaccg gagcgtccgg cgcatgccgg gcctggacgc
     1441 ggtgctgatc ttcctcgaag cgagcgacgc ggctctcctc aggcgattca gcgagacgcg
     1501 ccggccccac ccgctcgcgc acgatcggcc ggtgctcgac ggcatccgcg acgagcgccg
     1561 gcggctggcg cgcatccggg cgatggcgga cgagatcgtg gacacctcgt cgctcaccgt
     1621 gtacgacctg cgcgacgtgt tcgcggccca ctcgcgcggc ggcggcgccc cgggacggcc
     1681 gctccaggcc acgatcgtga gcttcggatt caagcacggc atccccgtcg aagccgacct
     1741 cctcttcgac gtgcggttcc tgccgaaccc cttcttccag ccgcggctcc ggccgctgac
     1801 ggggcgcgac cgccgcgtgg cggccttcat gcgggcccat cccctcacgc aggagacaat
     1861 ccgccgcatc gcctcgctcc tgcagttcct ggttccgcac tacgcggacg aaggcaagag
     1921 ctacctgacg ataggcatcg gctgcaccgg ggggcagcac cggtcggtgt tcatcgcgga
     1981 ggcgctgcgc cgggcgctcg ggcccgtcgg gggcgtgcgg ttccacgtgc gccaccgcga
     2041 cctcgcatcg gctggagagc cgggcgccgc cgggcggcgg cggcaaggag cacagacgtg
     2101 attggcgtcg tcgtcgtgac ccacggacag ctggcgacgg aactgctcaa cgcggccgag
     2161 gccatcgtcg gcgacctgcc ccggttcgcg gcggtgtcga tcggatggca cgacgacacc
     2221 gacgacgcgc gcgaggagat ccgccaggct atcgcgcgcc tgcagggcga gcacggggtg
     2281 ctggtgctga cggacatgtt cggcggcacg ccgacgaacc tcgggctgtc cttcgtcgag
     2341 cccgaccgcg tcgaggtcgt gacgggcgtg aacctgccca tgctgatcaa gctcgccagc
     2401 ctgcgagcgt cgcaggacct gctggcggtc gcccgccagc tccgcgaaac ggggcgcaac
     2461 gcgatccacg tggcgtcgga cctgctgcgg acggagccga agggctgaaa tgcggagccg
     2521 gacggtgacg atcgccaacg ccctcggcct gcacgcccgt gcggcggcgc gcttcgtgca
     2581 cgccgccagc gccttccagt gccgcgtgac cgtggtgcgc acggaccgcg cggagcggac
     2641 ggtggacgga aagagcatcc tggggctgct gctgctcgcc gcggcgaagg gcgccgccgt
     2701 gcgaatcacc gcggacggcc cggacgaggc cgaggcgctt gacgcgctgt gccgcttcgt
     2761 cgagtcggga ttcggggagg gcgcgtgctg acgctcgcgg gcctcggcgt gtcgccgggc
     2821 gtcgcctggg gcccggccgc gctgctgcgc cagcggcagc cggccgtgcg ctacctcatc
     2881 gcccccccgc gcgtcgccat ggaaaccggg cgcctggagg ccgcgcgaga ggcgacggcc
     2941 cgccagctgc gcgccgtgct ggcgaaggtg gccggcagcg ccgtggccga gttctcccac
     3001 ctgttcgagg cgcagctgct gatgctcgac gacgcgatgc tcgtcgcgcg ggcgaagagc
     3061 atcatcgcgc gcgagcgcat caacgccgaa tgggccctcg atcgcgcgtt cgacgagttc
     3121 agcgcgatct tcgcccatgt cgaggacgcg tacctccggg aacgccgagg ggacgtcgcc
     3181 gatgtcgtcg gacggctccg gcacaacctc ctcgccgccc accccggcga cgcggacttc
     3241 ctgcccgacc tggccgcacc cgccatcctc gtcgccgccg acctcgcccc gtcgttcgcg
     3301 gcccagatcg atcgccgcac gatcctcggc ctcgcactcg agacgggaag ccggacgcac
     3361 cactccgcca tcctcgcccg ttcgcttggc ctcccggccg tcgcgggcgc cgccggcgtg
     3421 acacggcaca tcctgccggg cacggtcgtg ctcgtggacg gcgaccgcgg cacggtcgtc
     3481 atcgatccga cgccggaggc gctggacgag gccgcggcgc gggccgaacg gctgcgcacg
     3541 cgggcggcga gcgcacgggc cgccgagcgc gggccggcgc tcacggccga cggagtcgcc
     3601 atcctgttcc
//
LOCUS       DAOSIF010000316         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22619_length_3609_cov_3.594823, whole genome shotgun sequence.
ACCESSION   DAOSIF010000316 DAOSIF010000000
VERSION     DAOSIF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22619_length_3609_cov_3.594823"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PLT35_10295"
     CDS             complement(<1..1446)
                     /locus_tag="PLT35_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOG29655.1"
                     /translation="MLPGRELTLDVVFGALRRWWWLLVLPLFAGIMGGLLYSRSQPSL
                     YRSDALIQVVPQRVPESYVSATVTERVEDRLRALAQQVLSRTQLEKLITDFGLFPAER
                     EKYPMEDVVELMTKRVLIQPLVAGQVSRRDSQSEAFRVSFDYENPAVAQKVVERLASF
                     FIDTNARDRGTQADQTSAFLEAQLADARSRLEAQEQRLKVFRERNAGRLPTQTQTNMQ
                     AIQNTQMALQSMVASLEVDRNRKLVLERLYNDAAADAPVAAPVPAGASGALPADAPPQ
                     QRLAAARAALAQMETRLSPKHPDIVRTRRVIADLEQQVAAAELERPVSPEAGQAVAAN
                     QEEARRRERLREMRAEIESLDRQIAFKEGEERRMRAEIGAYQARLEAVPGIESEWIAL
                     TRDYDTLQETYRQLLAKSENSKMAASLEQRQIGEQFRILDPPRVPQKPTSPNRLRINL
                     LGLLAGLGLGVALVGLFYVRDSTMRSEGDVLG"
     gene            complement(1466..3004)
                     /locus_tag="PLT35_10300"
     CDS             complement(1466..3004)
                     /locus_tag="PLT35_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29656.1"
                     /translation="MLSFKPGAWLTAALVMAAAGAGPAWARQGQSGPAPAAQQPAPQA
                     QPPAGAPEAPAAQQPAQPDAGARKPRRASRRTPRASGRQPAVVSQDFLDEARAPRDPG
                     PVRRELMLTASAMGGYDDNVTAGLGSGSFGRLVMASGSTADGEAALGYFVGNAARSLR
                     ADAAGTLMHYPKYLDKPAAGAFASLGATTRAGRRVTLRASERVGYEPFFSVYAPPGSG
                     GALPSAGAGGVRAAELFERRSLNTNTTAGIDTRWSPRNSTSLSYSYGVRRYTDDTDYG
                     DSRSHTADARYRLILSRAAAWNVDYRFQDLEYTYADALARPVRTHRIEAGPDIVKPFS
                     RRTGLRVSLAAGAGRVESWARAETGTIASWAMTGRASASLAVSPTWSIDADYRRDFSM
                     FQSVTDEVYTTDTARVSTGGLLGRRVALSLGASYSNWKTPYAGRISGTFDVYGASAQL
                     RVAVTRALAATAGYYYYRHRYSDPGALPEGFPAAFDRNAVRAGLSLAVPLAASSSQRP
                     LSQR"
     gene            complement(3013..>3609)
                     /locus_tag="PLT35_10305"
     CDS             complement(3013..>3609)
                     /locus_tag="PLT35_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOG29657.1"
                     /translation="AAGQAAEQTAPAQKPAGKPAAPQKPPTVPGPVVTTEPGFTIGPE
                     DVLGILVWREPELSGDVTVRPDGMITLPLVRDIRAAGLTPDELAERIQAAVREYVTDA
                     SVTVVVRQMNSRKAFITGEVARPGAYALTSSMTVMQLIAVAGGLNEFADGGKFSIMRV
                     ENGRTRALPFDYKNVANGRKAAQNILLRPGDTVVVPER"
BASE COUNT          443 a         1240 c         1405 g          521 t
ORIGIN      
        1 cccgagcacg tcgccctcgg agcgcatcgt cgagtcgcgg acgtagaaga ggccgaccag
       61 cgccacgccc aggccgaggc cggcgagcag gcccagaagg ttgatccgca gccggttcgg
      121 gctggtcggc ttctgcggca cgcgcggcgg gtcgaggatg cggaactgct cgccaatctg
      181 gcgctgctcg aggctggccg ccatcttcga gttctcgctc ttggcgagga gctggcggta
      241 cgtctcctgg agcgtgtcgt agtcgcgggt cagggcaatc cactccgact cgatgcccgg
      301 caccgcctcg aggcgcgcct ggtaggcgcc gatctcggcc ctcatgcggc gctcctcgcc
      361 ctccttgaag gcgatctgcc ggtcgaggct ctcgatctcg gcgcgcatct cgcggagccg
      421 ctcgcggcgg cgggcctctt cctggttcgc ggcgacggcc tggccggctt cgggcgacac
      481 cgggcgctcg agctccgcgg cggccacctg ctgctcgagg tcggcgatga cgcgcctggt
      541 gcgcacgatg tccgggtgct tcgggctcag gcgcgtttcc atctgcgcca gggccgcgcg
      601 ggcggcggcc aggcgctgct gcggcggcgc gtcggcgggg agcgcgccgc tcgcgccggc
      661 cggaaccggc gccgccacgg gcgcgtcggc ggcggcgtcg ttgtagaggc gctccaggac
      721 gagcttgcgg ttgcggtcca cctcgagcga ggccaccatc gactgcaggg ccatctgcgt
      781 gttctggatc gcctgcatgt tggtctgcgt ctgcgtgggc aggcggcccg cgttgcgctc
      841 gcggaacacc ttgagccgct gttcctgcgc ctcgaggcgc gagcgggcgt cggcgagctg
      901 cgcttcgagg aaggcgctcg tctggtcggc ctgcgtgccg cggtcgcggg cgttggtgtc
      961 gatgaagaag ctcgcgaggc gctcgaccac cttctgggcg acggcggggt tctcgtagtc
     1021 gaaggagacg cggaacgcct cggactgcga gtcgcggcgc gacacctgcc cggcgacgag
     1081 cggctggatg agcacgcgct tcgtcatcag ctcgaccacg tcctccatcg ggtacttctc
     1141 gcgctccgcc gggaagaggc cgaagtcggt gatgagcttc tcgagctgcg tgcggctgag
     1201 cacctgctgg gccagggcgc gcagccggtc ctcgacgcgc tcggtcacgg tggcgctcac
     1261 gtagctttcc gggacgcgct gcggcacgac ctggatcagg gcgtcggagc ggtagaggct
     1321 cggctgcgag cgcgagtaga gcaggccgcc catgatgccg gcgaagagcg gcaggacgag
     1381 cagccaccac cagcggcgga gcgcgccgaa gacgacgtcg agggtcagtt cccggcccgg
     1441 tagcatgagc agttcctcgc gagtgtcacc gctgggagag ggggcgctgg gacgagctgg
     1501 cggcgagagg gaccgccagc gagagccccg cgcggacggc gttgcgatcg aaggcggcgg
     1561 ggaagccctc gggaagagcc cccggatccg agtagcggtg ccggtagtag tagtagcccg
     1621 ccgtcgccgc cagcgcgcgc gtgacggcga ccctgagctg ggcggacgcg ccgtagacgt
     1681 cgaacgtccc gctgatcctg ccggcgtagg gcgtcttcca gttgctgtac gaggcgccga
     1741 ggctcagggc cacgcgccgg cccaggaggc cgcccgtgct cacgcgcgcg gtgtcggtcg
     1801 tgtacacctc gtcggtcacg ctctggaaca tcgagaagtc gcggcggtag tccgcgtcga
     1861 tcgaccacgt cggcgaaacg gcgaggctgg cgctcgcccg tcccgtcatc gcccaggacg
     1921 cgatggtgcc ggtttcggcg cgggcccagg actcgacgcg tccggcgccg gccgccagcg
     1981 acaccctgag gcccgtccgg cgcgagaacg gcttcacgat gtccgggccc gcctcgatcc
     2041 ggtgcgtgcg gacgggccgg gccagggcgt cggcgtaggt gtactcgaga tcctggaagc
     2101 ggtagtccac gttccaggcg gcggccctgg agaggatcag ccggtagcgg gcgtcggcgg
     2161 tgtgcgatcg gctgtcgccg taatcggtgt catcggtgta gcggcggacg ccgtacgagt
     2221 atgagagcga ggtcgagttc ctggggctcc agcgcgtgtc gatgcccgcg gtggtgttcg
     2281 tgttgagcga gcggcgctcg aagagctccg cggcccggac gccgccggcg ccggcgctcg
     2341 gcagcgcgcc cccgctgccc gggggcgcgt acacgctgaa gaacggctcg tacccgacgc
     2401 gctccgaggc gcggagcgtg acgcgccgcc cggcgcgggt cgtcgcgccg agggaggcga
     2461 aggcgccggc cgcgggcttg tcgaggtact tcggatagtg catgagcgtg cccgccgcgt
     2521 cggcccgcag ggaccgcgcg gcgttcccga cgaagtagcc gagcgcggcc tcgccgtccg
     2581 ccgtggaccc gctggccatg accaggcggc cgaagctgcc cgagccgagg cccgccgtca
     2641 cgttgtcgtc gtagcctccc atggcgcttg ccgtgagcat cagctcgcgg cgcaccggcc
     2701 cagggtcgcg cggcgcgcgc gcctcgtcga ggaagtcctg cgagacgacg gcgggctggc
     2761 gccccgaggc gcgcggcgtc cggcgcgagg cccggcgggg cttcctggcg ccggcatccg
     2821 gttgcgccgg ctgctgggcg gcaggggcct ccggggcgcc ggcgggcggc tgggcctgcg
     2881 gcgccggctg ctgcgcggcc ggcgcgggcc cggactggcc ctggcgcgcc caggcaggcc
     2941 ccgcccccgc cgcggccatc acgagcgccg cggtcagcca ggcgccaggc ttgaacgaga
     3001 gcatcgactg gattatcgct cgggaacgac gaccgtgtcg ccaggcctga gcaggatgtt
     3061 ctgcgcggcc ttcctgccgt tggcgacgtt cttgtagtcg aagggcagcg cgcgcgtccg
     3121 gccgttctcg acgcgcatga tcgagaactt cccgccgtcg gcgaactcgt tgaggccgcc
     3181 ggcgacggcg atgagctgca tgacggtcat cgacgaggtg agcgcgtacg cgccgggcct
     3241 ggcgacttcg ccggtgatga acgccttgcg gctgttcatc tggcggacga cgacggtgac
     3301 cgacgcgtcg gtgacgtact cgcgcacggc cgcctggatg cgctcggcca gctcgtccgg
     3361 cgtcaggccc gcggccctga tgtcgcggac gagcggcagg gtgatcatgc cgtcgggccg
     3421 cacggtgacg tcgccgctca gctcgggctc gcgccacacg aggatgccca gcacgtcctc
     3481 ggggccaatc gtgaagccgg gctcggtggt cacgaccggg cccggcacgg tcggcggctt
     3541 ctgcggggcc gcgggcttcc cggcgggctt ctgcgcgggc gcggtctgct ctgcggcctg
     3601 ccctgcggc
//
LOCUS       DAOSIF010000317         3589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22762_length_3589_cov_4.159875, whole genome shotgun sequence.
ACCESSION   DAOSIF010000317 DAOSIF010000000
VERSION     DAOSIF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22762_length_3589_cov_4.159875"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLT35_10310"
     CDS             <1..698
                     /locus_tag="PLT35_10310"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOG29658.1"
                     /translation="LRIVRGPAGKQDVFEARLEGKTLKGRVTYVNGRTNEFEGRRAPL
                     LLSRKAPVWGAPVKMFDGKTLDGWHARDPKAQNGWAVVDGELAVVQPKGNSDLVSDRT
                     FLDMKLHLEFNVEPKSNSGVYLRGRYELQILDNPDAKMALDTHGCGAVYSRLAPALDA
                     TKPAGEWQTLDVEFVGRQVKVTLNGQLVVNGVVEGITGGALSPFEEEPGPLMLQGDHG
                     KVRFRNIVVTPAT"
     gene            complement(682..1602)
                     /locus_tag="PLT35_10315"
     CDS             complement(682..1602)
                     /locus_tag="PLT35_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29659.1"
                     /translation="MIASVRAGLRAAASQPKLPLLVWGWYALLALVPILPVFSAWNAV
                     LGPSTEAQKILRRFDISVYADLVQDSAASAMGSPLRGLIAALAVAAISSAFVFGGTVE
                     VLGRGGEPGPFLQRFFSGGGRYFGRFLRLALVAAASALLAAGAVSAAVAGALSPLGGS
                     EWEPARYLAAALTAAAALATAGLFLLALDYARIQVARDGSRGMLKAYLGGLGFTLRNL
                     ASVFGVAIAFALMLAALALAYLAYEANTPAAGSWGAIAALSIVMQAIVFGRVFLRVAM
                     VGAERHLDLARRPAARPAPAGPSPGPTSPA"
     gene            complement(1626..>3589)
                     /locus_tag="PLT35_10320"
     CDS             complement(1626..>3589)
                     /locus_tag="PLT35_10320"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOG29660.1"
                     /translation="TASRQITGTETLTWRNPSAADAVSELQFHLYLNAFKNTRTTFMR
                     ESGGRMRGASAGAGSWGWIDVTSIRTAAGADLKPSLAFIQPDDGNPDDQTVARVLLPE
                     PVSPGRSVTLEISFTSQLPRVFARTGYEGDFYLAGQWFPKLGVYEPAGMRGRAASGWN
                     CHQYHAESEFYADYGTFTVEITVPSNFVVGATGARTSERRHANGTTTHVFEQADVHDF
                     AWTADPDYVVVKETFSAERDVTPAEYERTAKLLGRTLDEVRLSDVEITVLLQPDHRPQ
                     ARRHIEAAKAGLKWYGLWYGRYPYKTLTVVDPAPGASGAGGMEYPTFITAGTLWLFNR
                     WPFDRIRMLEEVTIHEFGHQFWYALVGNNEFEEAWLDEGLNTWSTAKAMLATYGRGRS
                     AVEFLGFTMDDEEALRVRNSPEARYNRILAAAWDYTPSSEYGFYAYEKPALALATLEG
                     YLGEQAMARLMRTYHERWRFKHPRSEDFFALVDEMTGRDWSWYFDQVVRGTDVVDYDV
                     GSASSVLVKRGRGVFDGPGGRTTVSAADAAKADAEAERTGKGVYRTVVVVRRSGGVRL
                     PVEVAFKFEGKPVERQAWDGRATFRKFVFERPEKLEWVDVDPDRRIALDANWLNNGRR
                     LRADSRPAASWIARWLFVIQHLITAVGLM"
BASE COUNT          557 a         1278 c         1256 g          498 t
ORIGIN      
        1 ccctccgcat cgttcgaggc ccggcaggca agcaggacgt cttcgaggcg cgcctggagg
       61 gcaagacgct caagggccgg gtcacgtacg tcaacggccg gacgaacgaa ttcgagggcc
      121 gcagggcccc gctcctgctc tcgaggaagg cgccggtctg gggcgcgccc gtgaagatgt
      181 tcgacggcaa aacgctcgac gggtggcacg cgcgggaccc gaaggcgcaa aacggctggg
      241 ccgtggtcga cggcgagctg gcggtggtgc aacccaaggg caactcggac ctggtgagcg
      301 accgcacgtt cctcgacatg aagctccatc tcgaattcaa cgtggagccg aagtcgaaca
      361 gcggcgtcta cctgcggggc cggtacgagc tgcagatcct cgacaacccg gacgcgaaga
      421 tggcgctcga cacccacggc tgcggcgcgg tgtacagccg cctcgcgccc gcgctcgacg
      481 cgacgaagcc ggccggcgag tggcagacgc tggacgtcga gttcgtcggc cggcaggtga
      541 aggtcacgct caacggccag ctggtcgtca acggcgtcgt cgaggggatc accggcggcg
      601 cgctgagccc gttcgaagag gagccgggcc cgctcatgct gcagggcgac cacggcaagg
      661 tccgcttccg caacatcgtc gtcacgccgg cgacgtaggc ccgggggacg gtcccgccgg
      721 cgcgggccgt gccgccgggc gcctggccag gtcgaggtgc cgttcggcgc cgaccatcgc
      781 gacgcgcagg aacacccggc cgaacacgat cgcctgcatg acgatcgaaa gggcggcaat
      841 ggcgccccag ctgccggcgg ccggcgtatt ggcctcgtag gcgaggtagg cgagcgccag
      901 cgccgccagc atcagcgcga acgcgatggc gacgccgaac acgctcgcca ggttccgcag
      961 cgtgaagccc agcccgccga ggtaggcctt caacatgccg cgggatccgt cgcgcgcgac
     1021 ctgaatgcgc gcgtagtcga gggcgagcag gaacaagccg gcagtcgcga gcgccgccgc
     1081 cgccgtcagg gcggcagcca ggtaccgggc gggctcccac tcggagccgc cgagcggcga
     1141 cagggccccg gccacggctg ccgacacggc gccggcggcg aggagcgcgg acgccgcggc
     1201 gacgagcgcg agcctgagga accggccgaa gtaccgtccg ccgccgctga agaaccgctg
     1261 caggaacgga cccggctcgc cccctctgcc caggacctcg acggtgccgc cgaagacgaa
     1321 ggcgctggag atcgccgcca cggcgagcgc cgcgatcagc ccgcgcagcg gcgagcccat
     1381 ggcgctcgcg gcgctgtcct gcaccaggtc ggcgtacacc gagatgtcga accgccgcag
     1441 gatcttctgg gcctccgtcg acgggcccag cacggcgttc cacgccgaga agaccggcag
     1501 gatcggcacg agggccagca gggcgtacca gccccagacg agcagcggga gcttcggctg
     1561 cgaggcggcc gctctcagac cggccctgac gctcgcgatc atcgtgatgc ctccggcagc
     1621 cggccctaca tcaggcccac ggcggtgatg aggtgctgga tgacgaacag ccaccttgcg
     1681 atccagctcg ccgccgggcg gctgtcggcc ctgagccgcc ttccgttgtt cagccagttc
     1741 gcgtccagcg cgatcctgcg gtccggatcc acgtcgaccc actccagctt ctccggccgc
     1801 tcgaagacga acttcctgaa cgtcgcgcgg ccgtcccagg cctggcgttc aacgggcttc
     1861 ccctcgaact tgaacgcgac ctcgaccggc aggcgcacgc cgccgctgcg ccggacgacg
     1921 acgaccgtcc ggtacacgcc cttccccgtg cgctcggctt cggcgtcggc cttcgcggcg
     1981 tcggcggcgg acaccgttgt gcgcccgccg gggccgtcga agacgccgcg cccgcgcttc
     2041 accaggacgg aggacgccga tccgacgtcg tagtccacca cgtcggtgcc gcgcacgacc
     2101 tggtcgaagt accagctcca gtcccggccg gtcatctcgt cgaccaaagc gaagaagtcc
     2161 tcgctgcgcg ggtgtttgaa ccgccaccgc tcgtggtagg tccgcatgag ccgcgccatg
     2221 gcctgttcgc ccaggtagcc ctcgagcgtc gccagcgcca gggccggctt ctcgtacgcg
     2281 tagaacccgt attcgctgga cggcgtgtag tcccaggcgg cggcgaggat ccggttgtag
     2341 cgcgcctccg ggctgttccg cacgcgcagc gcttcctcgt cgtccatcgt gaagccgagg
     2401 aactcgaccg ccgaccggcc gcgcccgtag gtcgccagca tcgccttggc cgtggaccac
     2461 gtgttgagcc cttcgtccag ccaggcttcc tcgaactcgt tgttgccgac gagcgcgtac
     2521 cagaactggt ggccgaactc gtgaatcgtc acctcctcga gcatgcggat ccgatcgaac
     2581 ggccagcggt tgaacagcca cagcgtgccc gcggtgatga aggtcgggta ctccatgccg
     2641 ccggcgccgc tcgcgcccgg cgccggatcc acgaccgtca gcgtcttgta gggatacctg
     2701 ccgtaccaga gcccgtacca cttcaggccc gccttggccg cctcgatgtg ccggcgcgcc
     2761 tgcggccggt gatcgggctg caggagcacg gtgatctcga cgtccgagag cctcacctcg
     2821 tccagcgtcc ggccgagcag cttcgcggtg cgctcgtact cggcgggcgt gacgtcgcgc
     2881 tccgccgaaa acgtctcctt gacgacgacg tagtccgggt cggccgtcca cgcgaagtcg
     2941 tggacgtcgg cctgctcgaa gacgtgcgtc gtcgtgccgt tggcgtgcct ccgctcgctg
     3001 gtgcgcgcgc cggtggcgcc gacgacgaag ttcgacggaa cggtgatctc gacggtgaag
     3061 gtgccgtagt cggcgtagaa ctcggattcg gcatggtact ggtggcagtt ccagccgctc
     3121 gccgcgcgcc cgcgcatgcc ggccggctcg tacacgccga gcttcgggaa ccactggccc
     3181 gcgaggtaga agtcgccctc gtagcccgtc cgggcgaaga cccgcgggag ttgggaggtg
     3241 aacgagatct ccagcgtcac cgatctgccg ggcgacacgg gttcgggcag aaggacccgc
     3301 gccaccgtct gatcgtccgg gttgccgtcg tcgggctgga tgaaggccag cgacggcttc
     3361 aggtcggcgc cggcggccgt cctgatggac gtgacgtcga tccagcccca cgagcccgcg
     3421 ccggccgacg cgccgcgcat ccggccgccc gactccctca tgaacgtggt cctcgtgttc
     3481 ttgaaggcgt tcaggtagag gtggaactgc agctcggaca cggcatccgc cgcggacggg
     3541 ttgcgccagg tcagcgtctc cgtcccggtg atctggcggc tggccgtgt
//
LOCUS       DAOSIF010000318         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22772_length_3588_cov_3.362581, whole genome shotgun sequence.
ACCESSION   DAOSIF010000318 DAOSIF010000000
VERSION     DAOSIF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22772_length_3588_cov_3.362581"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2175
                     /locus_tag="PLT35_10325"
     CDS             <1..2175
                     /locus_tag="PLT35_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG29661.1"
                     /translation="VPIITIPVSVLLAFIPMRLMGISSNIMSLAGIAISIGVLVDGAI
                     VEVENAYKRLEQWEHGGRRGDYHAVRLEALLEVGPSVFFSLLVIAVAFLPIFTLVDQE
                     GRLFTPLAWTKNLAMFIAAMLALTLDPALRMLFTRMDFPTWRPRALSWLVGQVTVGRY
                     YREERHPVSRALFAVYEPAARAVLRFPKLTIAAAVLIVASTVPVYQRLGHEFMPPLNE
                     GTILYMPTTLPGISVAEATRLLQTQDRILRSFPEVVTVFGKAGRAESSTDPAPFSMME
                     TTVILRPPSEWRRKARWYSSYAPEWLQASVLRRIWSDRLSWDELTAEMDAALQIPGVT
                     NAWTMPIKARIDMLTTGVRTPVGIKIFGPDLRQIESIGTRLEEIMRGVPGTRSVFAER
                     AAGGYFADIDVDREALARVGLSVGDVQDVIMSAIGGENVTTTVEGRARFPVNVRYPRG
                     LREDLDGLGRVLVMTPSGAQVPLRQLATIRTSAGPSMIRNENGLVAGYVYVDISTSDV
                     GGYVEHARAAVEAQMPTLPGGYSLEWSGQYENMLRVRERLKVVVPITIFLIFFLLYLN
                     TKSAFKAGVVMLAVPFSVVGAVWLMYALGYNVSIAAWVGMIALMGLDAETGVFMLLFL
                     DLSYDEARREGRLRTLAELKEAIIHGAVKRVRPKMMTVTAAMTGLMPIMWSTGAGADV
                     MKRVAAPMVGGLATSFLMELLVYPTIYLLWKRRELRREGGPI"
     gene            complement(2199..2579)
                     /locus_tag="PLT35_10330"
     CDS             complement(2199..2579)
                     /locus_tag="PLT35_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOG29662.1"
                     /translation="MVRSAIAAAAALMAAVLLAEPGAVLAQAGREQGKTVQVVRLSFD
                     DNGYVVTPSTVTKGVPVKMEVDLDTVKGCMRTIVIDAFGVKQTVKAGATTIAFTPTKS
                     GPIQIVCGMNMGKGQFTVADPASK"
     gene            complement(2742..>3588)
                     /locus_tag="PLT35_10335"
     CDS             complement(2742..>3588)
                     /locus_tag="PLT35_10335"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOG29663.1"
                     /translation="GGYYASLFGTLRNYWSCHLTAIGGPPAYTDRMTRGGPMMRSTAF
                     VRLSGEIEGDERKPVVWGVEGSYESARDGSWSGEGGASLKVKPLPTLSLEAGPTLARQ
                     LNAVQYVRAVADPAAAAMFGTRYVFGALDQTELGMETRINYIMNPRMSLQVYLQPLLS
                     VGRYTGFKEAIRPRTREFLEYGADAGAISYDAASRRYSVAPGGCGAPFSFPDPDFNYK
                     SLRVNAVFRWEFKPGSTLYAVWTQQRENQETDGPFRFGRSLSRLMEAPPDNVFMVKMS
                     YWFGR"
BASE COUNT          528 a         1241 c         1211 g          608 t
ORIGIN      
        1 gtgccgatca tcacgattcc ggtgtccgtc ctgctcgcgt tcatcccgat gcggctgatg
       61 gggatttcct ccaacatcat gtcgctcgcg ggcatcgcga tctcgatcgg cgtgctcgtc
      121 gacggcgcca tcgtcgaggt cgagaacgcc tacaagcggc tcgagcagtg ggagcacggc
      181 gggcgccggg gggactatca cgccgtcagg ctggaggcgc tgctggaagt cggcccgtcg
      241 gtgttcttct cgctgctcgt catcgcggtc gcgttcctgc cgatcttcac gctggtcgac
      301 caggaggggc ggctgttcac gccgttggcg tggacgaaga acctcgcgat gttcatcgcg
      361 gcgatgctcg ccctcacgct cgacccggcg cttcgcatgc tcttcacgcg catggacttt
      421 ccgacatggc gtccccgcgc gctgtcctgg ctcgtcgggc aggtgacggt cggccggtac
      481 taccgcgagg agcggcaccc ggtcagccgc gcgctcttcg cggtctatga gcccgccgcc
      541 cgggcggtgc tccggttccc gaagctgacc atcgccgccg cggtgctcat cgtcgcctcg
      601 acggtgccgg tgtaccagag actcggccac gagttcatgc ctccgctcaa cgagggcacg
      661 atcctctaca tgccgacgac gctgccgggc atctcggtgg ccgaggcgac gcggctgctc
      721 cagacgcagg accgcatcct caggtcgttt ccggaggtcg tcaccgtgtt cggcaaggcc
      781 ggacgcgctg agtcgtcgac cgatccggcg ccgttctcga tgatggaaac gaccgtcatc
      841 ctcaggcctc cgtccgagtg gaggcggaag gcgcggtggt actcctcgta cgccccggag
      901 tggctgcagg cgtcggtgct gcgccgcatc tggtccgatc ggctgtcctg ggacgagctg
      961 acggcggaga tggacgccgc gctccagatc cccggcgtca ccaatgcctg gaccatgccc
     1021 atcaaggcgc gcatcgacat gctgacgacg ggcgtccgga cgccagtggg gatcaagatc
     1081 ttcggccccg atctccggca gatcgagtcc atcggcaccc gcctcgagga gatcatgcgc
     1141 ggcgtccccg gcacgcgaag cgtcttcgcc gagcgcgccg ccggcgggta cttcgccgac
     1201 atcgacgtgg atcgcgaggc gctcgcccgc gtcggcctgt ccgtcggcga cgtgcaggac
     1261 gtcatcatgt ccgccatcgg cggcgagaac gtgacgacga ccgtcgaggg gcgtgcgcgc
     1321 tttcccgtca acgtcaggta cccgcgcggg ctccgggagg atctggacgg gctcggccgc
     1381 gtgctcgtca tgacgccgtc gggcgcgcag gtcccgctcc ggcagctcgc gacgattcgc
     1441 acctccgccg ggccctcgat gatccggaac gagaacggcc tggtggccgg ctacgtgtac
     1501 gtggacatct cgacgagtga cgtcggcggg tacgtcgagc acgcgcgcgc cgcggtcgag
     1561 gcgcagatgc cgaccctgcc tggcgggtac tccctcgagt ggagcggcca gtacgagaac
     1621 atgctgcgcg tgcgggagcg gctgaaggtc gtggtgccca tcacgatctt cctgatcttc
     1681 ttcctgctct acctgaacac gaagtccgcc ttcaaggcgg gcgtcgtcat gctggccgtg
     1741 ccgttctcgg tcgtgggcgc ggtgtggctg atgtacgccc tcggctacaa cgtgtcgatc
     1801 gcggcctggg tcggcatgat cgccctgatg ggcctcgatg ccgagacagg cgtgttcatg
     1861 ctgctcttcc tcgacctctc ctacgacgag gcgcgccgcg aggggcggct gcgcacgctc
     1921 gccgagctga aggaggcgat catccacggc gccgtgaagc gcgtccggcc gaagatgatg
     1981 accgtcacgg ccgccatgac tggcctcatg cccatcatgt ggtcgacggg cgccggcgcg
     2041 gacgtcatga agcgcgtcgc cgcgcccatg gtgggcggcc tggcgacgtc gttcctgatg
     2101 gagctgctcg tctacccgac gatctacctg ctctggaagc ggcgagagct ccgccgcgag
     2161 ggaggaccga tctgacggtg acggcgcccg ggtccgtcct acttggaggc ggggtccgcg
     2221 acggtgaact ggcccttgcc catgttcatg ccgcacacga tctggatcgg ccccgacttg
     2281 gtcggcgtga acgcgatggt cgtcgccccg gccttcaccg tctgcttcac gccgaaggcg
     2341 tcgatgacga tggtgcgcat acagcccttc accgtgtcga ggtccacctc catcttcacc
     2401 ggcacgcctt tcgtgacggt ggacggcgtc acgacgtagc cattgtcgtc gaacgagagc
     2461 ctgaccacct gcacggtctt cccctgctcc ctgcccgcct gcgccaagac cgcgccaggc
     2521 tccgccagca gcacggccgc catcagtgcc gcagcagccg cgattgcgct cctcaccatg
     2581 cattgcctcc cgggtcagcg gaccgctccg cacgccgacg gcgccgtcgg ccgcgcacgc
     2641 ctgtcgcgca aagtggcccc tccaacggat tgtagccgac atgaaggcct gcgccgcctg
     2701 gcggctgttc gcgcgcctgg cgggatggga cgggtgagga atcagcgccc gaaccagtaa
     2761 ctcatcttca ccatgaacac gttgtcaggc ggcgcctcca tcaggcgcga caagctgcgg
     2821 ccgaaccgga acggaccgtc ggtctcctgg ttctcgcgct gctgggtcca gacggcgtag
     2881 agggtggatc cgggcttgaa ctcccagcgg aacacggcat tgacgcgcag cgacttgtag
     2941 ttgaagtccg gatcgggaaa cgaaaacggg gcgccgcagc cgccgggggc gacgctgtac
     3001 cggcggctgg cggcgtcgta ggagatcgcg cccgcgtccg cgccgtactc caggaactcg
     3061 cgggtgcggg gccgaatcgc ctccttgaac ccggtgtagc gcccgaccga caggagcggc
     3121 tgcaggtaca cctggagcga catccggggg ttcatgatgt agttgatccg cgtctccata
     3181 cccagctcgg tctggtcgag ggccccgaag acgtagcgcg tgccgaacat ggcggcagcc
     3241 gccgggtcgg ccacggcccg cacgtactga acggcgttca actggcgcgc gagcgtcggg
     3301 ccggcttcga ggctgagcgt gggcagcggc ttcaccttca gcgacgcgcc gccttcgccc
     3361 gaccagctcc cgtcacgggc ggactcgtag gatccttcca ccccccagac caccggcttg
     3421 cgttcgtcgc cctcgatttc gccgctcaac cgcacgaagg cggtcgatct catcatcggt
     3481 ccgccacgcg tcatccggtc cgtgtacgct ggcggaccgc cgatggccgt caggtggcac
     3541 gaccagtagt tccggagcgt cccgaagagc gacgcgtagt atccgccg
//
LOCUS       DAOSIF010000319         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22829_length_3582_cov_3.888007, whole genome shotgun sequence.
ACCESSION   DAOSIF010000319 DAOSIF010000000
VERSION     DAOSIF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22829_length_3582_cov_3.888007"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLT35_10340"
     CDS             complement(<1..737)
                     /locus_tag="PLT35_10340"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOG29664.1"
                     /translation="MRIDLRSDTVTVPTPAMREAMAAAEVGDDVFGDDPTVNRLEALA
                     AGMAGKEAAVFVASGTMGNLASLLAHGGRGRDVILGDESHIYRYENGSASALGGLVFR
                     AVPTKADGTLPLEALRAAIHLPAHDYHYFHYTRPGVICLENTHNRCGGRVLAPDYFAE
                     VAALAAEHRLPVHLDGARLFNAAVAAGRPITDWTRHVSSVQICLSKGLSAPVGSVICG
                     DAAFVREARRMRKMLGGGMRQAGVIAAP"
     gene            complement(898..2046)
                     /gene="nagA"
                     /locus_tag="PLT35_10345"
     CDS             complement(898..2046)
                     /gene="nagA"
                     /locus_tag="PLT35_10345"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOG29665.1"
                     /translation="MLHIKNATVHTPDRVIERGSVLVEERRIVSVGPSADAAAAAGAR
                     EIDAAGLVLAPGFVELQLNGAFGHDFTDDPATIWQVGERITRYGVTSFLPTIITSPLE
                     QVAAGRKVVTDGRPAGYRGAVPLGLHVEGPFLNPKRKGAHNANYLRLPSLDAVADWSP
                     ATGVRLVTLAPELPGALDVIRALTARGVAVSIGHSDATFDQAVAGFDAGARYGTHLFN
                     AQSSLGHRDPGVPGALLSDDRITVGFIADGVHTHRAIIKIVYKVLGPSRMSLVTDAMA
                     ALGMAPGAHKLGDYDVIVDATSARLPDGVLAGSILALDQALRNVIEITGCGLSDALAM
                     MTSTPARLLGLDGALGRIAPGCAADLVLLSPDLRVRQTIVGGEVVFTA"
     gene            complement(2067..>3267)
                     /locus_tag="PLT35_10350"
     CDS             complement(2067..>3267)
                     /locus_tag="PLT35_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29666.1"
                     /translation="GANLMYAERLVKFLLWARGGWKVYVGGPASIGAHVARVYAADGP
                     RAFDHHFMGEQVHGRPFTVVSCAAEEVPAAREGGTPLGRHLDGCRIGFDLGASDLKVS
                     AVVDGEAIFSEEIVWEPVEQKDPAYHQGKIRDALRLAASKLPRVDAIGGSSAGVIVDN
                     RPMVASLFRGIPQERYGEIREMFLRFRDEFGVPLEVANDGDVTALAGAMSLDDNAVLG
                     VALGSSEAAGYVNPDGAITGWLNELAFAPVDYNPGAATDEWSGDAGVGALYFSQQCVF
                     RLAPSVGIDLPAGATKAEKLKHVQTRLEAGHEGATRIWQSIGVYLGYGIAHYADFYDL
                     RHVLILGRVTSGRGGTLILDGAKTVLADEFPDLAARVNIQLPDEKSRRVGQSIAAASL
                     PAIAKTS"
     assembly_gap    3268..3277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3278..3525)
                     /locus_tag="PLT35_10355"
     CDS             complement(<3278..3525)
                     /locus_tag="PLT35_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29667.1"
                     /translation="MASAFRLISPRHAPPLEPEFRPAILANRAFREEVAASGAGVPLA
                     LGLERPDGSLSRYDTVAFPEGHPRAGANTAWVPSPTAAT"
BASE COUNT          489 a         1308 c         1281 g          494 t
ORIGIN      
        1 ggcgcggcga tgacgccggc ctgccgcatc ccgccgccga gcatcttgcg catgcgccgc
       61 gcctcccgca cgaacgccgc gtcgccgcag atcaccgatc cgaccggcgc cgagaggccc
      121 ttggacaggc agatctgcac cgacgacacg tgccgcgtcc agtccgtgat gggccgcccg
      181 gccgccactg ccgcgttgaa cagccgcgcg ccgtcgaggt gcacgggcag ccggtgctcg
      241 gcggccaggg cggcgacctc ggcgaagtag tccggcgcca gcacgcgccc gccgcagcgg
      301 ttgtgcgtgt tctcgaggca gatgacccca ggcctggtgt agtggaagta gtggtagtcg
      361 tgcgcaggca gatggatggc cgcgcgcagc gcctcgagcg gcagcgtgcc gtcggccttc
      421 gtgggcaccg cccggaacac caggccgccg agggccgacg ccgatccgtt ctcgtagcgg
      481 tagatgtgcg actcgtcgcc gaggatcacg tcgcggccgc ggccgccgtg cgcgagcagc
      541 gacgccaggt tgcccatcgt gccgcttgcg acgaacacgg cggcctcctt gcccgccatc
      601 cccgccgcca gcgcctccag ccggttcacg gtcggatcgt cgccgaacac gtcgtcgccc
      661 acctccgccg cggccatggc ctcgcgcatg gcgggcgtgg gcaccgtcac ggtgtcgctt
      721 cgcaggtcga tacgcatgtc tcctccgccg ccgccggccg cgcgcgcgat cccggcggcc
      781 gcgcggccgc gcggccttcg agatcccaac cggccccgcg ccatcaccgc ggaggaagag
      841 gagccggtct cctctggcgt ttgatcggcg atgaagcggc cgggcccggc tcccgtgcta
      901 ggcggtgaac accacctcac cgccgacgat cgtctggcgg acgcgcaggt ccggcgacag
      961 gagcaccagg tcggccgcgc accccggagc gatccggccc agggccccgt cgaggccgag
     1021 cagccgggcc ggcgtcgacg tcatcatcgc cagagcgtcg gacaggccgc acccggtgat
     1081 ctcgatgacg ttccgcagcg cctggtcgag cgcgaggatg ctgccggcga gcacgccgtc
     1141 cggcaggcgc gcgctcgtgg catccacgat gacgtcgtag tcgccgagct tgtgcgcgcc
     1201 gggggccatg ccgagcgccg ccatcgcgtc ggtcaccagg ctcatccggg acggtccgag
     1261 caccttgtag acgatcttga tgatcgcccg gtgggtgtgg accccgtcgg cgatgaaccc
     1321 gaccgtgatg cggtcgtccg agagcagggc gccgggcacg cccggatcgc ggtgcccgag
     1381 cgaggactgg gcgttgaaca ggtgcgtgcc gtagcgcgcg ccggcgtcga acccggcgac
     1441 ggcctggtcg aacgtcgcgt cggagtggcc gatgctgacg gcgacgccgc gggcggtcag
     1501 cgcgcggatc acgtcgagcg cgcccggcag ttccggcgcc agcgtcacca gccgcacgcc
     1561 cgtcgccggc gaccagtccg ccacggcatc gagggacggc agccgcagat agttcgcgtt
     1621 gtgcgcgccc ttccgcttcg ggttgaggaa cggcccttcc acgtgcaggc cgagcgggac
     1681 cgcgccgcgg tagcccgccg ggcgcccgtc ggtgacgact tttcggcccg ccgcgacctg
     1741 ctccagcggg gatgtgatga tcgtcgggag gaacgaggtg acgccgtagc gcgtgatccg
     1801 ctctcccacc tgccagatcg tcgccgggtc gtcggtgaag tcgtggccaa aggcgccgtt
     1861 gagctggagt tccacgaacc cgggcgccag cacgaggccc gccgcgtcga tctcccgcgc
     1921 gccggcagcg gcggccgcgt ccgcagacgg cccgacggac acgatgcgcc gttcctccac
     1981 caggaccgag ccgcgctcga tgacgcggtc cggcgtgtgt actgtggcgt tcttgatgtg
     2041 cagcatctcc cgcgctcctc tgtccgtcag ctcgtcttgg cgatcgccgg caggctcgcg
     2101 gccgcgatcg actgcccgac gcgacggctc ttctcgtccg gcagctggat gttcacgcgc
     2161 gcggcgaggt ccgggaactc gtcggcgagc accgtcttgg cgccgtcgag gatgagggtg
     2221 ccgccccgcc ccgaggtgac gcgcccgagg atcagcacgt gccgcaggtc gtagaagtcg
     2281 gcgtagtgcg cgatgccgta ccccaggtag acgccgatgc tctgccagat cctcgtggcg
     2341 ccttcgtggc ccgcctcgag gcgcgtctgc acgtgcttca gcttctccgc cttggtggcc
     2401 ccggcgggca ggtcgatgcc gacggagggc gccagacgga agacgcactg ctgcgagaag
     2461 tagagcgcgc ccacccccgc gtctccagac cactcgtcgg tcgccgcgcc ggggttgtag
     2521 tccaccggcg cgaacgccag ctcgttgagc cagcccgtga tggcgccgtc cggattgacg
     2581 tagccggcgg cctcgctcga tccgagcgcg acgcccagca ccgcgttgtc gtcgaggctc
     2641 atcgccccgg caagcgccgt gacgtcgccg tcgttggcca cctcgagcgg gaccccgaac
     2701 tcgtcgcgga accgcaggaa catctcccga atctcgccgt agcgctcctg cgggatcccc
     2761 cggaagagcg acgcgaccat cggccggttg tccacgatga cgccggccga gctgccgccg
     2821 atcgcgtcca cgcgcggcag cttcgacgcg gccagcctca gcgcgtcgcg gatctttccc
     2881 tggtggtacg ccgggtcctt ctgctcgacg ggctcccaaa caatctcctc gctgaagatc
     2941 gcctcgccgt ccacgaccgc cgacaccttc aagtccgacg ccccgaggtc gaagccgatg
     3001 cggcacccgt cgaggtgccg gccgaggggc gtgcctccct cgcgcgcggc gggaacctcc
     3061 tcggccgcac aggaaacgac tgtgaagggc cggccgtgga cctgctctcc catgaagtgg
     3121 tggtcgaagg ccctggggcc gtcggcggcg tagacccggg cgacgtgcgc gccgatggac
     3181 gcgggccccc cgacgtagac cttccagccg cccctggccc agagcaggaa cttgacgagg
     3241 cgctcggcgt acatcaggtt cgcgccgnnn nnnnnnngtg gccgcggtcg gcgacggcac
     3301 ccatgcggtg ttcgcgccgg cgcgcgggtg cccttccggg aaggcgacgg tgtcgtagcg
     3361 cgacagcgac ccgtccgggc gctcgaggcc gagggcgagc gggacgcccg cgccggaagc
     3421 ggcaacctcc tcgcgaaacg cgcggttggc gaggatcgcg ggccggaact ccggctcgag
     3481 gggcggggcg tgtcggggac tgatcaagcg gaacgctgaa gccatggtgt ctctctccga
     3541 acgcgtgcag gtgatgtcaa gccgccgggc gggggcccct ct
//
LOCUS       DAOSIF010000320         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22898_length_3573_cov_3.787948, whole genome shotgun sequence.
ACCESSION   DAOSIF010000320 DAOSIF010000000
VERSION     DAOSIF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22898_length_3573_cov_3.787948"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLT35_10360"
     CDS             <1..561
                     /locus_tag="PLT35_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29668.1"
                     /translation="VWSAWIAAWLAFRGRLSRTRRPAAAVCAIVVAVAYIGAMIAGSR
                     IAERQARRVAAERGWTVHEAAAMPLAAQPLRRDVIVATPGRYEFVSVNWLLGSVAGVE
                     PRPLARGERGDPVVNAALAAPFVSGVRAWLRFPAFEVRSLPDGGRRVLIRDARFAVGT
                     PSGYGVVAIVDLDRAGTPRPGPHPGR"
     gene            complement(577..1305)
                     /locus_tag="PLT35_10365"
     CDS             complement(577..1305)
                     /locus_tag="PLT35_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29669.1"
                     /translation="MTVNGVEEEAMGPVVRAGLVVAASLVLAAASAAADGIQAQPPAA
                     LESLAGTWEGAAQTPDGEAVLKAVFTVVDGKLSGVIESSLGPIAVGTAMLADAKLTIA
                     FEYQGSPGTLTGTVQGSRIEGVWAVMDASGTFALSRRDERASDPISGDWEGEASVAGE
                     VMPFSMSLRVSGEIVTGEISSAAGAVPITSGSWRDGVLALAFPYLAGEPVTMSGALKD
                     GRLSGTVDYNRSEAVGTFSAVRKR"
     gene            complement(1321..2058)
                     /locus_tag="PLT35_10370"
     CDS             complement(1321..2058)
                     /locus_tag="PLT35_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG29670.1"
                     /translation="MIRDVLTVAWKEWRELMSLSGGARGGRLSLVILLAVFGVLLPLQ
                     SGADWVRSASTVFYWAWVPLMLVGNAIADAIAGERERHTLETLLASRLPDPAILLGKV
                     LAAVSYGWGLVMGMLLLSLVTVNMSARSDGLLLFPARIAVGAPLLALLGAGLAAAAGV
                     LVSLRAATVRHAAQALSVGILLLIFVPVFLAESMPDAWKARAASWLMTAGADRIAWSA
                     AAGLAGLDLALMGLALARFRRVRLIVD"
     gene            complement(2055..2984)
                     /locus_tag="PLT35_10375"
     CDS             complement(2055..2984)
                     /locus_tag="PLT35_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG29671.1"
                     /translation="MSSAIIRTDQLTRAFGGIKAVDGLDLEVPAGEVFGFLGPNGAGK
                     TTTIRLLLGLLEPSGGSARVLGYDTRTEAGAIRQRAGALLEHHGLYERLSAEDNLAFY
                     ARIWGLGAAERRNRVRELLESLGLWERRREAVGKWSRGMRQRLAVARALLHRPALLFL
                     DEPTAGLDPVAASHLREQLAALVAREGVTVFLTTHNLAEADRLCQRVGVIKQGRLVAV
                     GHPDELKARSATRLEIRGRAFTDALVAALSRQPGVERAALMNGRMVIDMAEAADSSPL
                     VAMIVGSGAAVDEVRRDKSSLEDVFLTLMEEDR"
     gene            3093..>3573
                     /locus_tag="PLT35_10380"
     CDS             3093..>3573
                     /locus_tag="PLT35_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG29672.1"
                     /translation="MRRALASVLTRLIARAARPLIALRYRIEIRGLEAVRERGRRGIL
                     FLPNHAALLDPVFLVVYLFPSFRVRPLADEHQVGRTAFGRLALLYGSRILPNLERSGA
                     GARARMREAITDIANGLEAGENILFYPAGHLKRGYLEVIGSASGTETLVKTVPSARTV
                     "
BASE COUNT          514 a         1348 c         1223 g          488 t
ORIGIN      
        1 gtctggtccg cgtggatcgc cgcatggctg gcgttccgcg gccgcctcag caggaccagg
       61 cggccggcgg cggctgtctg cgcgatcgtc gtggccgtgg cgtacatcgg cgcgatgatc
      121 gccgggtcga ggatcgcgga gcggcaggcc cgccgcgtcg cggccgagcg cggctggacg
      181 gtgcacgagg cggcggccat gccgctggcc gcccagccgc tgcgccggga cgtcatcgtg
      241 gccacgcccg ggcgttacga gttcgtctcc gtcaactggc tgctggggtc cgtcgcgggt
      301 gtggagccgc gccccctcgc gcgcggcgag cggggcgacc ccgtcgtcaa cgcggcgctg
      361 gcggcgccgt tcgtcagcgg cgtccgcgcc tggctgcgct tcccggcgtt cgaggtccgg
      421 agcctgcccg acggcgggcg ccgcgtcctc atccgcgacg cccggttcgc cgtcggaacg
      481 cccagcggct acggcgtcgt ggccatcgtc gacctcgacc gcgccggcac gccgaggccg
      541 ggcccgcacc ccgggcgcta agcgccgcgg ccgggcctat cgcttcctca cggcgctgaa
      601 cgttcccact gcctcgctgc ggttgtagtc caccgtgcct gaaagcctgc cgtccttcag
      661 ggcccccgac atggtgaccg gctcgccggc caggtaggga aacgccagcg ccagcacgcc
      721 gtccctccac gatccgctcg tgatggggac ggcacccgcc gccgaggaga tctctcccgt
      781 gacgatctca ccgctcacac gcagggacat ggagaacggc atgacctcgc ccgccacgct
      841 cgcctcgcct tcccagtcgc cgctgattgg atccgacgcg cgctcgtcgc gccgggacag
      901 cgcgaacgtg ccgcttgcgt ccatcactgc ccacacgcct tcgattcgcg acccctggac
      961 ggtgcctgtc aatgtcccag gcgacccttg gtactcaaag gcgatcgtca gcttggcgtc
     1021 ggccagcatg gccgtcccga cggcaatcgg cccgagtgac gattcgatga cgccgctcag
     1081 cttgccgtcc acgacggtga acacagcttt caggacagcc tcgccatcag gcgtctgcgc
     1141 ggccccctcc cacgtgccgg ccagcgactc cagcgcggcc ggcggctgtg cctgaatgcc
     1201 gtcagccgcg gctgaagctg ccgcgagcac gagcgatgcc gccaccacca accctgcgcg
     1261 cacgacgggt cccatcgcct cctcctccac tccgttcacg gtcacgtcag ccagaacgag
     1321 tcagtccacg atcaagcgca cccggcggaa ccgggcgagc gccaggccca tgagcgccag
     1381 gtcgagcccc gccagccccg cagccgccga ccacgcgatc cgatccgcgc cagccgtcat
     1441 cagccagctt gcggcccgag ccttccaggc gtccggcatg gactcggcca gaaagacggg
     1501 cacgaagatc aacaggagaa tgccgacgct cagggcctgg gccgcgtggc ggaccgtcgc
     1561 cgcgcgcaac gacaccagca cgccggcggc cgcggcaagt cctgcgccga gcagcgcgag
     1621 aagcggcgcg ccgacggcga tgcgggcagg aaagagcagg agcccgtcgc tccgggccga
     1681 catgttcacg gtgaccaggc tgagcaggag catgcccatg accaggcccc agccgtagct
     1741 cacagcggcg aggactttgc cgagcaggat ggcggggtcc ggcagtcggc tggcgaggag
     1801 cgtctcgagc gtgtgacgtt cccgctctcc ggcaatggcg tcggcaatcg cgttgccgac
     1861 cagcatcagc ggcacccacg cccagtagaa caccgtcgac gccgagcgga cccagtcggc
     1921 gcccgactgg aggggcagga gcacgccgaa caccgccagc aggatcacga ggctcaggcg
     1981 gccgccgcgc gcgcccccgg acaggctcat cagctcgcgc cactctttcc aggccaccgt
     2041 cagcacgtcg cggatcaccg atcttcctcc atgagcgtca ggaacacgtc ctcgagcgag
     2101 ctcttgtcgc ggcggacctc gtcgacggcc gctcccgacc cgacgatcat cgccacgagc
     2161 ggcgacgagt cggcagcctc ggccatgtcg atgaccatcc ggccgttcat caaggcggcg
     2221 cgctcgaccc ccggctggcg ggacagcgcg gcgacaaggg cgtcggtgaa ggcgcggcct
     2281 cggatctcga gccgggtggc gctgcgcgcc ttgagctcgt ccgggtggcc gacggcgacg
     2341 agccggccct gcttgatgac gccgacgcgc tggcagagcc ggtcggcctc ggcgaggttg
     2401 tgggtcgtca ggaacaccgt gacgccttcc cgcgcgacca gcgcggccag ctgctcccgg
     2461 aggtgagagg ccgcaacggg gtccagtccc gccgtgggtt cgtcaaggaa cagcaaggcg
     2521 ggccggtgga gcagcgccct ggccacggcg agccgctgtc tcatgccccg gctccacttc
     2581 cccaccgcct cgcggcgccg ctcccagagc cccagggact cgagcagttc gcgcacgcga
     2641 ttccggcgct cggcggctcc caggccccag atccgggcgt agaaggcgag gttgtcctcg
     2701 gcgctcaggc gttcgtacag cccgtggtgc tcgagcagcg cgcctgcccg ctggcggatg
     2761 gcgccggcct cggtccgcgt gtcgtatccg agcacgcggg cactcccccc gctcggttcc
     2821 agcaggccga gcaggagcct gatggtcgtc gttttccccg ccccgttcgg gccgaggaag
     2881 ccgaagacct cgccggcggg cacctcgaga tccaggccat ccacggcctt gatcccgccg
     2941 aacgcgcgcg tcagctggtc ggttcggatg atcgccgacg acatggcccc ctatgatacc
     3001 cggactgcca cggcacgcgc gcgcgtcggc ccgcggccgg ttgcgccacg gcgcggagtc
     3061 gagtacgttc aacgggtcgc tgaggtgccg acatgcgcag agctcttgca tccgtcctga
     3121 cgcggctcat cgcccgcgcc gcgcgccccc tgatcgcgct gcggtaccgc attgagatcc
     3181 ggggcctcga ggccgtccgg gagcgcgggc gccgcggcat cctgttcctg ccgaaccacg
     3241 cggccctgct ggaccccgtg ttcctcgtgg tctacctgtt cccgtccttc agagtccgcc
     3301 cgttggccga cgagcatcag gtggggcgga cggcgttcgg caggctcgcg ctgctctatg
     3361 gctcgcggat cctgccgaac ctggagcgga gcggcgccgg cgcccgcgcc cgcatgcgcg
     3421 aagcgatcac ggacatcgcc aacggcctcg aggccgggga gaacatcctc ttctaccccg
     3481 ccggccacct gaagcgcggt tacctcgagg tcatcgggag cgcgagcggg acggagacgc
     3541 tggtgaaaac cgtgccttcc gcccggaccg tac
//
LOCUS       DAOSIF010000321         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_22953_length_3566_cov_3.572486, whole genome shotgun sequence.
ACCESSION   DAOSIF010000321 DAOSIF010000000
VERSION     DAOSIF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22953_length_3566_cov_3.572486"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLT35_10385"
     CDS             <1..282
                     /locus_tag="PLT35_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29673.1"
                     /translation="TLTTAAAAKAFGVALPPSFARQLAGLLARPSLRAAFWTDLGALA
                     APLAWPFVLGSLAGATALGALAYAVARPSIEAGRRRRALRRPDALPEVR"
     gene            282..785
                     /locus_tag="PLT35_10390"
     CDS             282..785
                     /locus_tag="PLT35_10390"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOG29674.1"
                     /translation="MQVSARWHHLVGTGVFALAMVLLYQVTTVDSRYAARQAVLREAS
                     AVPAPGARLRFSATAYCKGEVTASGVRPRTGVAAADPALLPVGSVIQVVDEQPYTGIY
                     TIMDTGPLVQGRHIDLYMWSCTEALAFGRRAVDVTVLRLGWNPRTSTSGLLPARPLPV
                     TTLPIPR"
     gene            complement(802..2322)
                     /locus_tag="PLT35_10395"
     CDS             complement(802..2322)
                     /locus_tag="PLT35_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOG29675.1"
                     /translation="MTSNGHQTMVAVLEDDQVVEVSVERERQRGVVGNLYKGRVSRVL
                     PGMQCAFVDIGLERDGFLYVSDVVDTMDAFGRLEGGDDEGDEAAADPAEAGGRAKDDE
                     PKIEALLKAGQEVLVQVAKEPLGTKGARLTCHVTMAGRFLVFLPTVDHVGVSRKIEAR
                     DERSRLRSIVRAFREAEGFAGGVIIRTAAAGRPEADLVADLAYFKRAWEEIRRNLDAS
                     TCPAVVYREQGLVAKMIRDLLSEDYSAIRIDNAAEYRRVRDLVQRIMPSLTPRVKHYT
                     KVFPIFQEYGVQSEIDKALRSKVWLKSGGYIVINQTEALVAIDVNTGRFVGKRNDRLE
                     DTIVKTNIEAVKEIVRQIRLRDLGGIIVLDFIDMEERKNRQKVSQALDQELARDRSPS
                     KAVQVSDFGLIIVTRKRVKQSLERVMTQPCPYCSGTGVIRSSATICYEILDEVAKLGS
                     GVEGLGVQLRVNPDIARALREEEAAVLKELEAAVGTRVIVKADPHLHHEQFDVMVL"
     gene            complement(2340..3440)
                     /gene="rodA"
                     /locus_tag="PLT35_10400"
     CDS             complement(2340..3440)
                     /gene="rodA"
                     /locus_tag="PLT35_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOG29676.1"
                     /translation="MFDRRLCAHIDWLMLGAVYLLCAIGVVMIASATADPSRATASQL
                     PATQVYALVLGTGALLVCLVIDYRSFGERAHLIYGGLVLLLVYVLEFGSVRGGGRRWI
                     ALPWFNLQPSEFMKIGLALVLARLFSETRHQAPPGRELAIAGALTAVPVVLVARQPDF
                     GTAVTIASVVLGVAILAGMRLKLLGLLLAAALLTAPAAWTFALQDYQRGRILSFVNPS
                     RDPLGAGYQQIQARITVGSGGLWGKGFRKGTQGQLSFLPVAHNDFIFSVLAEEQGFVG
                     VLVVLALYAFVVLRAVDTARRARDRLGALLVVGVLSSFLFQVVYNVSMSAGLAPVKGL
                     TLPLMSYGGSSLIATLAGVGLILNVRMRRFTN"
BASE COUNT          507 a         1322 c         1223 g          514 t
ORIGIN      
        1 acgctcacga cggcggcggc ggcgaaggcg ttcggggtgg cgctgccgcc ctcgttcgcg
       61 cgccagctgg ccggcctgct cgcccgcccg tccctgaggg ccgccttctg gaccgatctc
      121 ggagcgctcg ccgcgccgtt ggcgtggccg ttcgtgctgg ggtcgctggc cggcgcgacc
      181 gccctcggag cgctggccta cgcggtcgct cggccgtcca tcgaggcggg tcggcggcgg
      241 cgcgccctcc gccggccgga cgccctcccg gaggtgcggt gatgcaggtc tccgcccgct
      301 ggcaccacct cgtcggcacc ggcgtcttcg cgctggccat ggtgctgctg taccaggtga
      361 cgaccgtgga ctcgcgctac gccgcgcggc aggcggtgct gcgcgaggcg tcggccgtgc
      421 cggcccccgg cgcccgcctg cgcttcagcg cgaccgccta ctgcaaggga gaggtcaccg
      481 cgtccggcgt ccggccgcgc accggcgtcg ccgccgccga cccggcgctg ctcccggtcg
      541 ggtcggtcat ccaggttgtc gacgagcagc cgtacaccgg catctacacc atcatggaca
      601 ccggcccgct ggtgcagggg cgccacatcg atctctacat gtggagctgc accgaggccc
      661 tcgccttcgg ccggcgcgcc gtggacgtga ccgtcctgcg gctcggctgg aacccgagga
      721 cgagcacgtc cggactgctg ccggcgcggc cgctgccggt gacgacgctc cccatcccgc
      781 gctaacgcgg ccggggccgg ctcagagcac catcacgtcg aactgctcgt ggtgcaggtg
      841 cggatcggcc ttgacgatca cccgcgtgcc gacggccgcc tccagctcct tcagcaccgc
      901 ggcctcctct tcccggagcg cccgggcgat gtccgggttg acgcgcagct ggacgcccag
      961 cccctccacc ccgctgccga gcttggccac ctcatcgagg atctcgtagc agatcgtcgc
     1021 cgacgaccgg atgacgcccg tgcccgagca gtaggggcac ggctgcgtca tcacgcgctc
     1081 caggctctgc ttcacccgct tgcgcgtcac gatgatcagc ccgaagtcgg acacctggac
     1141 ggccttcgac ggcgagcggt cccgggcgag ctcctgatcc agcgcctgcg agaccttctg
     1201 ccggttcttc cgctcctcca tgtcgatgaa gtcgagcacg atgatgccgc cgagatcccg
     1261 gagccggatc tggcggacga tctccttcac ggcctcgatg ttggtcttga cgatcgtgtc
     1321 ctcgaggcgg tcgttccgct tgccgacgaa gcgcccggtg ttgacgtcga tggcgacgag
     1381 cgcctcggtc tggttgatga cgatgtaccc gccggacttg agccagacct tgctgcgcag
     1441 ggccttgtcg atctcggact ggacgccgta ctcctggaag atcggaaaga ccttcgtgta
     1501 gtgcttcacc cgcggcgtga gcgagggcat gatgcgctgc acgagatccc ggacgcggcg
     1561 gtactccgcc gcgttgtcga tgcggatcgc cgagtagtcc tcgctcagca ggtcccggat
     1621 catcttggcg accaggccct gctcccggta cacgacggcc gggcacgtcg aggcgtccag
     1681 gttccggcgg atctcctccc aggcccgctt gaagtacgcc aggtcggcga ccaggtccgc
     1741 ctccggccgg ccggccgcgg cggtgcggat gatcacgccg ccggcgaacc cctcggcctc
     1801 gcggaacgcg cgaacgatgc tccgcagccg cgatcgctcg tcgcgggcct cgatcttccg
     1861 cgacacgccg acgtgatcga cggtcggcag gaagacgagg aagcgcccgg ccatcgtcac
     1921 gtggcacgtc aggcgggcgc ctttcgtccc gagcggctcc ttcgccacct ggacgaggac
     1981 ttcctgcccc gccttgagga gcgcctcgat cttcggctcg tcgtccttgg cccgcccgcc
     2041 cgcctccgcc gggtccgccg cggcctcgtc gccctcgtcg tctccaccct cgaggcgccc
     2101 gaaggcgtcc atggtgtcca cgacgtcgga cacgtagagg aagccgtccc gctcgaggcc
     2161 gatgtccacg aaggcgcact gcatgccggg cagcaccctg gagacgcggc ccttgtagag
     2221 gttgcccacg accccgcgct ggcgctcgcg ctcgacggac acctccacga cctggtcatc
     2281 ctcgagcacg gcgaccatcg tctggtggcc gttggacgtc acgatcatct ccttgtccac
     2341 tagttcgtga acctccgcat cctcacgttg agaatcagcc cgacgcccgc gagggtcgcg
     2401 atgagggacg atccgccgta gctcatcagc ggcagcgtca gccccttgac cggcgccagt
     2461 cccgcggaca tcgagacgtt gtagacgacc tggaagagga agctggacag cacgcccacg
     2521 accagcagcg ccccgaggcg gtcgcgggcg cgccgggccg tgtccacggc cctcagcacg
     2581 acgaacgcat acagggcgag gacgaccagc acgcccacga agccctgctc ctcggcgagc
     2641 accgagaaga tgaagtcgtt gtgcgcgacg gggaggaagc tcagctggcc ctgcgtgccc
     2701 ttccggaagc ccttgcccca caatccgccg gacccgacgg tgatccgggc ctggatctgc
     2761 tggtagccgg ccccgagggg gtcgcgcgac gggttgacga agctcaggat ccgcccgcgc
     2821 tggtagtcct gcagggcgaa ggtccacgct gccggggcgg tgagcagggc ggcggccagg
     2881 aggagcccga gcagcttcag ccgcatgccc gcgaggatcg ccacgccgag caccaccgac
     2941 gcgatggtca ccgcggtgcc gaaatccggc tgccgggcga ccagcacgac cggcacggcg
     3001 gtgagggcgc cggcgatggc gagttcgcgc cccggggggg cctggtgccg ggtctcgctg
     3061 aagagccgcg cgagcaccag ggccaggccg atcttcatga actcggaggg ctgcaggttg
     3121 aaccacggca gcgcgatcca gcggcgtccg ccgccgcgca cgcttccgaa ctcgagcacg
     3181 tagacgagca gaaggaccag gccgccgtag atgaggtggg cgcgctcgcc gaaggaccgg
     3241 tagtcgatca ccaggcacac gagcagggcg cccgtgccga gcaccaaggc gtagacctgc
     3301 gtcgccggca gctgcgaggc cgtcgcccgc gacgggtccg ccgtcgcgct ggcgatcatc
     3361 acgaccccga tggcgcacag caggtacacc gcgcccagca tgagccagtc gatgtgcgca
     3421 cacagccgcc ggtcgaacat ggcgctcccc gctcctactg ccctgcaccc gcgccgcgcg
     3481 ccgccgcggg cgccggcgcg cccggcggcg cctcgaaggc cggcagcggg cggccttccc
     3541 gtttggcgaa gtacgtctcc aggacg
//
LOCUS       DAOSIF010000322         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23087_length_3549_cov_3.904121, whole genome shotgun sequence.
ACCESSION   DAOSIF010000322 DAOSIF010000000
VERSION     DAOSIF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23087_length_3549_cov_3.904121"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLT35_10405"
     CDS             <1..611
                     /locus_tag="PLT35_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29677.1"
                     /translation="ETFDQAIAALSELYSEKHAYRTVVLDTLDWLERLIWSDVCKKRG
                     VESIEDIPYAKGYVLALTQWREFLEGLNALRNERGMMVILLAHAKIEKFENPETETYD
                     RYVPRLHKLASAVVQEWCDEVLFATYKVFIKKLDEGFSQTRTQGIGTGERIIRTSERP
                     AHVAKNRLNLPDELPLDWNVYAKHLVATPVVAAVNKGGKSNG"
     gene            604..1059
                     /locus_tag="PLT35_10410"
     CDS             604..1059
                     /locus_tag="PLT35_10410"
                     /inference="COORDINATES: protein motif:HMM:NF016896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HOG29678.1"
                     /translation="MANLQGFDARTVEPSTDFEAIPAGKYVAIITDSGMKETKAGTGN
                     YLELTFQIVEGEYKGRNLWARLNLDNPNAVAVKIAQGELSAICRAVGVMAPNDSCELH
                     NLPLMITVKCKKREDTGEITNEVKGYAKKEATAGQPQQAAAQTPPWARR"
     gene            1056..1436
                     /locus_tag="PLT35_10415"
     CDS             1056..1436
                     /locus_tag="PLT35_10415"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HOG29679.1"
                     /translation="MIEFDLPYPPSVNHYYRRVGPRTLISREGRRFRERVLSVLAATR
                     PRPFDGPIAVQVEVYPPDHRRRDIDNVQKSLFDALQHGGVYRDDSQIVRLQIEKRGCV
                     PGGMTIVRIEPLADAAPARTGGST"
     gene            1459..3162
                     /locus_tag="PLT35_10420"
     CDS             1459..3162
                     /locus_tag="PLT35_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG29680.1"
                     /translation="MMQLRPYQQDAVDAVYAHLRERDDNPVVAIPTGGGKTPVIATMC
                     RDAVGRWNGRVLILAHVKELLEQALDKLQQVAPEMWHQIGVYSAGLKSRDTEHPIIVA
                     GIQSVYRRACDLDRFDLVIVDEAHMIPPDGDGMYRTFLEEARKINPNLRVIGLTATPF
                     RMTTGPICGPDNVLNSVCYEIGVRELIVQGYLCQLVSKGGREDVDTGGLHVRGGEFMA
                     DEVEALMDKEERVESACKEIVDYTRDRNSCLVFTSGVQHARHVAEVLRRSEPRVETVF
                     GETSDEDRARAIGEFRAGRVKYLVNVNVLTHGFDAPNVDCVAMLRPTMSPGLYYQMAG
                     RGFRLCDSKKNCLILDFGGNVLRHGPVDAIRTDADRKGDGDGEAPAKKCPQCNALIAM
                     GYATCPQCGFVFPPPERRMHDAKAGTAGILSDHVEIEEHAVQDVRYSVHEKRDAPPDA
                     PRTMRVEYQIGWQRYISEWICVEHTGYARAKAESWWRRRSNVPIPDTAEEAVRLAEAG
                     ALCGTQSITVRSVAGEKYDRITGYALGEKPSYREPGWDDEEPVPVTANGEEVDDDDLP
                     F"
     gene            3143..>3549
                     /locus_tag="PLT35_10425"
     CDS             3143..>3549
                     /locus_tag="PLT35_10425"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HOG29681.1"
                     /translation="MTISPSDGLLLESALAYARLGYAVFPCMPGGKTPATKHGCKDAT
                     TDEARIAAWWRKQPNANIGLATTGLIVVDVDGDDNPWLKDQPDLQQTLALAPLSLTPS
                     GGRHHIFRQPNDRAWGNTTGRLAPKVDTRGDGG"
BASE COUNT          685 a         1166 c         1188 g          510 t
ORIGIN      
        1 ccgagacgtt cgatcaggcc atcgcggccc tgtcggagct gtactccgaa aagcacgcct
       61 accgcaccgt cgtcctggac acgctcgact ggctcgagcg gctgatctgg tcggacgtgt
      121 gcaagaagcg cggggtcgag agcatcgagg acatccccta cgcgaagggc tacgtcctgg
      181 cgctgaccca gtggcgcgag ttcctggaag ggctgaacgc gctgcggaac gagcgcggga
      241 tgatggtcat cctgctggcg cacgccaaga tcgagaagtt cgaaaacccc gagacggaga
      301 cctacgatcg ctatgtcccg cgcctgcaca agctggcgtc ggcggtggtc caggagtggt
      361 gcgacgaggt cctgttcgcc acctacaagg tcttcattaa gaagctcgac gaggggttca
      421 gccagacgcg cacgcagggc atcggcaccg gcgagcggat catccgcacg tccgagcggc
      481 ccgcccatgt ggcgaagaac cggctgaacc tgccggacga gctgccgctg gactggaacg
      541 tgtacgcgaa acacctggtt gcgacgcctg tcgtcgcggc cgtcaacaag ggaggaaaga
      601 gcaatggcta acctgcaagg attcgatgcc cgcaccgtgg aaccgtcaac cgacttcgag
      661 gctattccgg ccgggaagta cgtggcgatc atcaccgact cggggatgaa ggagaccaaa
      721 gccggaaccg gcaactatct ggaactgacc ttccagatcg tcgagggcga gtacaagggg
      781 cgcaacctct gggcgcgcct gaacctcgac aacccgaatg cggtcgcggt caagatcgcc
      841 cagggcgaac tgtcggccat ctgccgcgcc gtcggggtca tggccccgaa cgactcgtgc
      901 gaactgcaca acctgcctct catgatcacc gtgaaatgca agaagcgcga ggacacgggc
      961 gagatcacga acgaggtcaa gggctacgcg aagaaggaag cgacggcggg gcagcctcag
     1021 caggccgccg cgcagacacc tccctgggcg cgccgatgat cgagttcgat ctgccgtacc
     1081 cgccgtcggt gaatcactac taccggcgcg tggggccgag gacgctcatc agccgcgagg
     1141 gccgcagatt ccgggaacgg gtcttgtcgg tcctcgcggc gacgcgcccg aggccgttcg
     1201 acggacccat cgccgttcag gtcgaggtct atccccccga ccaccggcgg cgggacatcg
     1261 acaacgtgca gaagagtctc ttcgacgcgc tccagcatgg aggcgtctac cgggacgaca
     1321 gccagatcgt gcggctccag atcgagaagc gcggctgcgt tcccggcggc atgaccatcg
     1381 tccgcatcga gccgttggcg gacgccgccc cggcccgaac gggaggttcg acgtgatcgg
     1441 agatgaaccg gaaaccccat gatgcagttg cgaccatacc agcaagacgc cgtggatgcc
     1501 gtctacgccc acctgcgcga gcgggacgac aacccggtcg tcgcgatccc tacgggcggc
     1561 ggcaagacgc cggtgatcgc cacaatgtgc cgcgacgccg tcggccgctg gaacggccgc
     1621 gtcctgatcc tggcgcacgt caaggaactc ctggaacagg ccctcgacaa gctccagcag
     1681 gtcgcgccgg agatgtggca ccagatcggc gtctactcgg ctggactcaa aagccgggac
     1741 acggaacacc cgatcatcgt ggcgggcatc cagagcgtct atcggcgcgc ctgcgacctc
     1801 gaccgcttcg atctcgtgat cgtggacgag gcccacatga tcccaccgga cggcgacggc
     1861 atgtaccgga cgttcctgga ggaagcccgg aagatcaacc ccaacctccg ggtgatcgga
     1921 ctcacggcca ccccgttccg catgacgacc ggccccatct gcgggccgga caacgtgctg
     1981 aactcggtct gctacgagat cggcgtccgt gagctgatcg ttcagggcta cctctgccag
     2041 ctcgtctcca agggcggacg cgaggacgtc gacaccggcg gtctgcatgt ccggggcggc
     2101 gagttcatgg cggacgaggt cgaggcgctc atggacaagg aagagcgcgt cgagtccgcc
     2161 tgcaaggaga tcgtcgatta cacccgcgac cggaactcgt gtctggtctt caccagcggc
     2221 gtccaacacg cccgacacgt cgccgaggtc ttgcgccggt ccgagccgcg cgtcgagaca
     2281 gtcttcggcg agacatccga cgaggatcgc gcccgggcca tcggcgagtt ccgcgccggg
     2341 cgcgtgaagt acctggtcaa cgtcaacgtc ctgacgcacg ggttcgacgc gccgaacgtg
     2401 gactgcgtgg cgatgctgcg gccgacgatg tcgccggggc tctactacca gatggccggg
     2461 cgcggattcc ggctctgcga cagcaagaag aactgcctga tcctggactt cggcgggaac
     2521 gtcctgcggc acgggcccgt ggacgcgatc cgcaccgacg cggaccggaa aggcgatggc
     2581 gacggcgagg ccccggcgaa gaagtgtccg cagtgcaacg cgctgatcgc catggggtac
     2641 gcgacgtgcc cccagtgcgg cttcgtcttc ccgccgcccg aacggcggat gcatgacgcc
     2701 aaggcgggca cggccggaat cctctcggat catgtcgaga tcgaggagca cgccgtccag
     2761 gacgtgcggt acagcgtcca cgagaagcgc gacgccccgc cggacgcgcc gcgcaccatg
     2821 cgcgtcgagt accagatcgg ctggcagcgg tacatctcgg agtggatctg cgtcgagcat
     2881 accggctacg cccgcgcgaa agccgagtcg tggtggaggc ggcgctcgaa cgtgcctatc
     2941 cccgacaccg ccgaggaagc ggtccgtctg gccgaggccg gggcgctctg cggcacgcag
     3001 tccatcacgg tgcgcagcgt cgccggtgag aaatatgacc ggatcaccgg ctacgcgctc
     3061 ggcgagaagc cgtcctaccg cgagcccggc tgggatgacg aggagccggt cccggtgacc
     3121 gccaatggag aggaggttga cgatgacgat ctccccttct gacggtctgc ttctggaatc
     3181 ggctcttgcc tatgcccggc tgggttacgc cgtctttccc tgcatgcccg gaggcaagac
     3241 cccggcgacc aagcatggct gcaaggacgc gaccaccgac gaggcccgaa tcgcggcgtg
     3301 gtggcggaag cagccaaacg ccaacatcgg gcttgcgacc acgggtctga tcgtcgtgga
     3361 cgtggacggc gatgacaatc cctggctcaa agatcagcca gacctgcaac agacgctggc
     3421 cctggctccg ttatcgctga cgcccagcgg cggtcggcat cacatcttcc ggcaaccgaa
     3481 cgaccgcgca tggggaaaca cgaccggcag gcttgcgccc aaggtcgaca cgcgcggtga
     3541 cggcggata
//
LOCUS       DAOSIF010000323         3541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23150_length_3541_cov_3.629662, whole genome shotgun sequence.
ACCESSION   DAOSIF010000323 DAOSIF010000000
VERSION     DAOSIF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23150_length_3541_cov_3.629662"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="bamD"
                     /locus_tag="PLT35_10430"
     CDS             complement(<1..635)
                     /gene="bamD"
                     /locus_tag="PLT35_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOG29682.1"
                     /translation="MPRRSRRVALPGLFLVALGLAPGCAGNRNPVPPGILEGDRYLFE
                     RAAELVARKKWSQARAHYVQLVDNYPQSAYRPDAKLGLGDTYFGENTVESLVLAQNEY
                     REFLTFYPTNPRADYAQFRIGMCHFGQMQSAERDQTATKDAIAEFTAFLERFPESPLV
                     PDAQTKLREAQTRLTDSHFKVGYFYYRSGWYPGAISRFQAVLKDDPDFPYR"
     gene            complement(762..1181)
                     /locus_tag="PLT35_10435"
     CDS             complement(762..1181)
                     /locus_tag="PLT35_10435"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG29683.1"
                     /translation="MTPIRVRYAETDQMRIAYHANYLAWFEVGRCEWLRDLGWSYRAL
                     EERDGVMLPVIEACCRYRIPARYDDELEIRARGVLLSPARVRFDYQVVRQEDGAVLAE
                     GHTTHASTDLGGRPRRLPEHVRRLFALPAGTAPSPSM"
     gene            complement(1196..1894)
                     /gene="rpe"
                     /locus_tag="PLT35_10440"
     CDS             complement(1196..1894)
                     /gene="rpe"
                     /locus_tag="PLT35_10440"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG29684.1"
                     /translation="MSVRLAPSILAADFAALGASVAAAERGGADMIHVDVMDGRFVPN
                     ISVGPLVVRALRRVTSLPLDVHLMIEDPDRHIPAFAEAGASLISVHVEALPHLHRTLA
                     FIKGLGLKAGAVLNPSTPVGSLEEVAGDVDFVLLMTVNPGFGGQTLIPRSLSKIQAMR
                     SLLDEAGNAAAIEVDGGIDDRTVSRVVAAGADWLVAGTAVFRSDDAEAAARRLRAAAV
                     AAAQERLASPRGPR"
     gene            complement(1891..2613)
                     /locus_tag="PLT35_10445"
     CDS             complement(1891..2613)
                     /locus_tag="PLT35_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOG29685.1"
                     /translation="MSRSARARSFGRTVLIAAGLLATYALFAAGAMRVALRAREVVVP
                     ALVGLEFIEAGRAMDEAGLTIKVDDSRPFSPTVAAGRIASQDPAAGAVVRRGRSVRVW
                     LSAGSRASIVPALVGESERTAQARVLQEGFEISSVAEIRSSDLPPGIVVAQDPAPGSR
                     HTRVGVLVNRGERAAGFVMPDLIGTSADAAVAVLRSHGLRVSVVSQQPYPGTPPGIVL
                     RQSPSAGFQAAPDQPISLEVSR"
     gene            complement(2667..>3541)
                     /locus_tag="PLT35_10450"
     CDS             complement(2667..>3541)
                     /locus_tag="PLT35_10450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOG29686.1"
                     /translation="WMARYGFGTALEWVRFNNAPAPLTLRVVAPSASRDEAASALRAS
                     GVGARACRFAADGLIVESGQPLAGPLARSGLFLAQDEASQLVGAFAAPEPGERVLDVC
                     AAPGGKTIQFAAALGGRGFLVAGDVRPRRVRLLLGALAAARAGGVGVVRHDALRGLPY
                     AAVFDRVVVDAPCSSLGTIRRDPDVRWRRTEADLLDLAGRQLLMLAEAARTVRPGGRL
                     VYATCSSEPEENESVVEAFLASAPGFVLEDPSLGPRRVDPGVAACLDDRGCLRTTPHQ
                     HGLEAYFAARLRRV"
BASE COUNT          557 a         1293 c         1280 g          411 t
ORIGIN      
        1 tcccggtacg ggaagtccgg atcgtccttc agcaccgcct ggaagcgcga gatggcgccg
       61 gggtaccagc cagaccggta gtagaagtac ccgaccttga agtgcgagtc ggtcagccgc
      121 gtctgggcct cgcgcagctt cgtctgcgcg tccggcacga gcgggctctc gggaaaacgc
      181 tccaggaagg ccgtgaactc ggcaatggcg tccttggtcg cggtctggtc gcgctcggcg
      241 gactgcatct gcccgaagtg gcacatcccg atcctgaact gcgcgtagtc ggcgcggggg
      301 ttggtcgggt agaaggtcag gaactcgcgg tactcgttct gggcgaggac gagcgactcg
      361 accgtgttct cgccgaagta ggtgtccccg agccccagct tcgcgtccgg ccggtaggcg
      421 ctctgcgggt agttgtccac cagctgcaca taatgggcgc gcgcctgcga ccacttcttc
      481 cgggccacga gctcggcggc gcgctcgaag aggtacctgt cgccttcgag gatgccggga
      541 ggaaccgggt tccggtttcc ggcacacccg ggcgcgagcc cgagggccac gaggaacagg
      601 cctggcagcg cgacacgacg cgaacgacgc ggcataggca cgatcccgat cccgcggggt
      661 ccgggcgcag gaagatcccg aacccgccgc ccccggccgg ccgccagccg gcccccccgg
      721 gggcatgacg aggccgccgc gcaggcggcc agattcccat tctacatcga cggcgacggc
      781 gccgttcccg caggaagggc gaacaaccgc ctgacgtgct ccggcagccg cctgggccgg
      841 cctcccaggt ccgtcgaggc gtgggtcgtg tggccctcgg ccagcaccgc gccgtcctcc
      901 tgccggacga cttggtaatc gaagcggacg cgcgcgggcg agaggagcac gcctcgcgcg
      961 cggatctcga gctcatcgtc gtagcgtgcc gggatgcggt accggcagca cgcctcgatg
     1021 acgggcagca tcacgccgtc gcgctcctcg agggcccggt acgaccagcc cagatcccgc
     1081 agccactcgc accggccgac ctcgaaccag gccaggtagt tggcgtggta ggcgatgcgc
     1141 atctggtccg tctcggcgta gcgcacgcga atgggcgtca ccgaggtgcg cccgctcacc
     1201 gcggcccccg gggcgacgcc agacgctcct gcgcggccgc cacggccgcc gcccgcaggc
     1261 gcctcgcggc ggcctcggca tcgtcggacc ggaagaccgc cgtgcccgcc accagccagt
     1321 ccgcgcccgc ggcgaccacg cgggaaaccg tgcggtcgtc aatgccgccg tcgacctcga
     1381 tggcagcggc attcccggcc tcgtcgagca aggaccgcat cgcctggatc ttcgacaggc
     1441 tgcgcgggat gagggtctgg ccgccgaagc ccgggttcac cgtcatcagc aggacgaagt
     1501 cgacgtctcc cgccacctcc tcgagcgaac ccacgggcgt cgaggggttc aagacggcgc
     1561 cggccttgag gcccaggccc ttgatgaacg ccagcgtgcg gtgcaggtgc ggcagcgcct
     1621 cgacgtgcac cgagatcagc gacgccccgg cctcggcaaa cgcggggatg tgccggtcgg
     1681 ggtcctcaat catgaggtgc acgtccagcg gcagcgacgt gacccgtcgc agcgcccgga
     1741 caacgagggg gcccaccgag atgttgggca cgaagcgccc gtccatcaca tcgacgtgga
     1801 tcatgtcggc gccgccgcgc tcggcggcgg cgacggaggc gccgagggcc gcaaagtccg
     1861 cggcgaggat cgacggggcg aggcgcacgc tcatcggctc acctccagcg agatcggctg
     1921 gtccggggcc gcctggaacc cggcggacgg cgattgccga agcacgatgc ccggcggcgt
     1981 accgggatag ggctgctgcg agacgacgct cacgcggagg ccgtggctgc ggaggacggc
     2041 cacagccgcg tcggccgacg tgccgatgag atcgggcatg acgaagcccg ccgcccgctc
     2101 gccgcggttc acgaggacac cgacgcgcgt gtggcggctg cccggggccg ggtcctgcgc
     2161 cacgacgatc ccgggcggaa gatcggacga tcggatctcc gccacggagg agatctcgaa
     2221 cccctcctgc agcacccggg cttgcgccgt ccgctcggac tcaccgacga gcgcgggcac
     2281 gatggaggcg cgggagccgg cgctcagcca cacgcgcacg ctgcgccccc gtcgcacgac
     2341 ggctcccgcg gccgggtcct gcgacgcgat gcgcccggcc gcgacggtcg ggctgaaggg
     2401 ccggctgtcg tccaccttga tcgtcagacc cgcctcgtcc atggcccggc ccgcctcgat
     2461 gaactcgagc cccaccagcg ccggaacgac cacttcccgg gcgcgcaagg cgacccgcat
     2521 ggcgcctgcg gcaaagagcg cataagtggc cagcaggccg gccgcgatga ggacggtgcg
     2581 cccgaacgag cgtgcgcgcg cgctgcgaga catcgctggc ggcactgtac tacacgagcg
     2641 gcgcgcccca ccaccggcca ggagcgctag acccgccgaa ggcgggcggc gaaatacgct
     2701 tcgaggccgt gctgatgcgg cgtcgtgcgg aggcagccgc gatcgtcgag gcaggccgcc
     2761 acgcccggat cgacgcggcg gggccccaga ctcggatcct cgaggacgaa tcccggcgcg
     2821 gacgcgagaa acgcctcgac gaccgactcg ttctcctcgg gttcgctcga gcacgtggcg
     2881 tagacgaggc ggccgccggg gcgcaccgtg cgcgcggcct cggccagcat gaggagctgg
     2941 cgtccggcaa ggtccaggag atccgcctcg gtgcggcgcc atcggacgtc gggatcgcgg
     3001 cggatcgtcc ccaggctcga acacggcgca tccacgacca cgcgatcgaa cacggccgca
     3061 taggggaggc cgcggagcgc gtcgtgccgc acgacgccta cgcccccggc gcgcgcggcc
     3121 gcaagggccc cgaggaggag gcggacgcgc cggggccgga catcgcccgc gaccagaaac
     3181 ccgcggccgc cgagggcggc agcgaactgg atcgtcttcc cgcccggagc cgcgcacacg
     3241 tcgaggacac gctcgcccgg ctccggcgcc gcgaacgcgc caacgagctg ggacgcctca
     3301 tcctgggcga ggaacagccc ggatcgcgcc agagggccgg caagcggttg tccggactcc
     3361 acaatcaggc cgtccgccgc gaagcggcag gcgcgcgcgc ccacaccgga ggcgcgcagc
     3421 gccgaggccg cctcgtcgcg ggaggcggaa ggggcgacga ccctcagcgt gagcggcgcg
     3481 ggcgcattgt tgaacctcac ccactccagc gccgtcccga acccgtagcg cgccatccat
     3541 c
//
LOCUS       DAOSIF010000324         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23223_length_3531_cov_3.591772, whole genome shotgun sequence.
ACCESSION   DAOSIF010000324 DAOSIF010000000
VERSION     DAOSIF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23223_length_3531_cov_3.591772"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..533
                     /locus_tag="PLT35_10455"
     CDS             210..533
                     /locus_tag="PLT35_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29687.1"
                     /translation="MAFTYHELKAKNISELRDIAKGLKHEAVQGYTQLNKEHLLVALC
                     QALNVSVHEHHEAVGIDKAMLKATIRALKRERDAAIAAGDHAKLKAVRRQIHGVNRQI
                     KKHLV"
     gene            538..1722
                     /locus_tag="PLT35_10460"
     CDS             538..1722
                     /locus_tag="PLT35_10460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG29688.1"
                     /translation="MIVPLSDAPNPGGRPFVTYALIAANVLLYVGISLPLSGQPADTS
                     SPAFRAYVEVVRDQLPAGVPIGAVLQHVSAYDLFVFEHGYKPAAPQFADLLFSLFLHG
                     GFLHLLGNMLFLWIYGDNVEYRLGRLQFLFWYIVTGIAATLAFAAFARGSGVPLVGAS
                     GAISGLLGFYFRWFPRNTVRLFVFLPPFIMNVVAVQARTVLGIYLVADNLLPALVTGG
                     GGGGVAHGAHIGGFVAGLMAAWAIDRWEVRPATTAFPAGLPARKPEEQAAAAGGEEMA
                     RLASSYFALPADRTRRALEPRASLALANWLAGNGHSHAALVVYRRHLRDYPADATTAA
                     AHLGAGMVQMEQMGQVAPAYQHFLEALDLDPDEDTAARARRALARIAATQKYNVGRPR
                     PS"
     gene            complement(1730..2734)
                     /locus_tag="PLT35_10465"
     CDS             complement(1730..2734)
                     /locus_tag="PLT35_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29689.1"
                     /translation="MRRSIVAAGTLCLAVALTAGLGADVKSSHKTQVKFEGMFGRAIS
                     MFSGKSAKEGVVSTTAVAGDRMMSVTGQSGELVDLAEEKVYQIDFKDRSYKVKTFAEI
                     RKEWEEAQAKMKEQAAAQKPAEQPDAEAQMEVDFSVNRTAERETINGYDCQKTVMTIA
                     VRQKGRKLEESGGLVMTNDLWMAPEIAALKEEAAFQLRYMKKLLGNDAATMARDLAQA
                     MAMYPMMKPAMERMQKESGKLSGTPIRTVMRVETVASPEQAQSQGDQPKPSIGGVAGK
                     LGGLFGRKKKDDDAPAEAPAPAGAKNHSTLLTSTSELVSVSTAVTAEDVAIPAGFKQK
                     "
     assembly_gap    2915..2924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2925..>3531)
                     /locus_tag="PLT35_10470"
     CDS             complement(<2925..>3531)
                     /locus_tag="PLT35_10470"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOG29690.1"
                     /translation="HDIQQPGLRSHDEGFYAGTTSVQAADEEGWVVSVTPSGGWVPAV
                     VAGRTGVGLSQRMQAFVLDEADCPFNVVRPGQRPRSTLTPSLALKDGQPYLAFSVQGG
                     DSQDQNLLQFFLNMVEFGMNVQQACEAPNINSFQMRSSFDEHLSEPGRMLVAESTPPE
                     ARRQLEAMGYTLTFSRLTSGPITAIMFDRKHGTMWGGASNYG"
BASE COUNT          470 a         1262 c         1173 g          616 t
ORIGIN      
        1 aacacggagt cctccctccg tagaccggtg ccggaagacg atgccgcggg gcttgtgtag
       61 ttgtagtgtc ggcttctctc ctctctctgt caaggggcac cgggccgcgc tccgcgcgcg
      121 ccgggacggc ccccgccggt ggccgacctg gcgcgccgcg acccggtccg cagttcggat
      181 agaatgccca cggaaaggag cgcgcgcaca tggcattcac gtatcacgag ctgaaggcga
      241 agaacatctc cgagctgcgc gatatcgcga agggcctgaa gcacgaggcg gtgcagggct
      301 acacgcagtt gaacaaggag catctgctcg tcgcgctctg ccaggcgctg aacgtgtccg
      361 tccacgagca ccacgaggcg gtcggcatcg acaaggcgat gctgaaggcg acgatccgcg
      421 cgctgaagcg ggaacgcgac gccgccatcg ccgccggaga ccatgcgaag ctgaaggcgg
      481 tgcggcgcca gatccacggc gtgaaccgcc agatcaagaa gcacctggtg taggcccgtg
      541 atcgtcccgc tgagcgacgc gccgaacccg ggcgggcggc ccttcgtcac ctacgcgctc
      601 atcgcggcga acgtcctcct gtacgtgggg atctccctgc cgctcagcgg gcagccggcc
      661 gacacgagca gcccggcgtt ccgcgcgtac gtcgaggtgg tgcgcgacca gcttcctgcc
      721 ggcgtgccga tcggggccgt cctgcagcac gtgtccgcgt acgacctctt cgtgttcgag
      781 cacggctaca agcccgcggc cccgcagttc gccgacctgc tgttctcgct cttcctccac
      841 ggcgggttcc tgcacctgct ggggaacatg ctgtttctct ggatctacgg cgacaatgtc
      901 gagtaccggc ttgggcggct ccagttcctg ttctggtaca tcgtcaccgg catcgcggcg
      961 acgctggcgt ttgcggcctt cgcgaggggc tccggcgtgc cgcttgtcgg cgcctcgggc
     1021 gcgatctccg ggctgctggg gttctacttc cgatggttcc cgcgcaacac ggtgcggctg
     1081 ttcgtcttcc tgccgccctt catcatgaac gtcgtggccg tgcaggcccg caccgtcctg
     1141 gggatctacc tggtggcgga caacctgctg ccggcgctgg tcacgggcgg aggcggcggc
     1201 ggggtggccc acggtgcgca catcggcggg ttcgtggccg gcctgatggc ggcctgggcg
     1261 attgaccggt gggaagtccg gcccgcgaca acggcgttcc ccgccggcct gccggcgcga
     1321 aagccggagg agcaggcggc ggccgccggt ggggaagaga tggccagact ggcgtcgtcc
     1381 tacttcgcgc ttccggcgga ccggacgcgg cgggcgctcg agccccgcgc ctcgctggcc
     1441 ctggcgaact ggctggcggg caacggccac tcgcacgcgg cgcttgtcgt gtaccggcgc
     1501 cacctccgcg actacccggc ggacgcgaca acggccgcgg cgcacctcgg cgccgggatg
     1561 gtgcagatgg agcagatggg ccaggtggcg ccggcgtacc agcacttcct ggaggcgctc
     1621 gacctcgacc ccgacgaaga cacggcggcg cgcgccagac gcgccctggc gcgcatcgcc
     1681 gcgacgcaga agtacaacgt cggccgcccc cggccgtcct gaggcgcgtt tacttctgct
     1741 tgaatcccgc cgggatcgcc acgtcttccg cggtgaccgc ggtcgagacg ctgacgagtt
     1801 cgctcgtcga ggtcagcagc gtcgagtggt tcttcgcgcc ggcgggcgcc ggcgcctccg
     1861 ccggggcgtc gtcgtccttc ttcttgcgtc cgaacagccc gccgagcttg ccggccaccc
     1921 cgccgatact cggcttcggc tggtcgcctt gcgactgcgc ctgttccggc gaggccaccg
     1981 tctccacccg catcacggtc cggatgggcg tgccgctgag cttgccggac tccttctgca
     2041 tgcgctccat cgccggcttc atcatcgggt acatcgccat ggcctgcgcg aggtcgcgcg
     2101 ccatggtggc ggcgtcgttg cccagcagct tcttcatgta gcggagctgg aacgcggctt
     2161 cctctttcag ggccgcgatc tccggggcca tccacaggtc gttcgtcatg accagcccgc
     2221 cgctctcctc tagcttcctg cccttctggc gcacggcgat ggtcatcacg gtcttctggc
     2281 agtcgtagcc gttgattgtc tcgcgctcgg cggtcctgtt cacgctgaaa tccacttcca
     2341 tctgcgcttc ggcgtcgggc tgctccgccg gtttctgcgc ggccgcctgc tccttcatct
     2401 tcgcctgcgc ctcttcccac tccttgcgga tttcggcaaa ggtcttgacc ttgtagctcc
     2461 tgtccttgaa gtcgatctgg tagaccttct cctcggcgag gtcgaccagc tcgccggact
     2521 gccccgtcac cgacatcatg cggtcgcccg cgacggccgt cgtgctgacg acgccttcct
     2581 tggccgactt gcccgagaac atgctgatgg cccggccgaa catcccctcg aacttcacct
     2641 gggtcttgtg gctcgacttg acgtccgccc cgaggccggc cgtgagcgcg acggcgaggc
     2701 acaacgtgcc cgccgcgaca atcgatctcc gcatgtgagt catcctcctg tccagtccat
     2761 gacgccgccg tcggcgctgc cgcggcgcgg gagcgcggac cggctggttt tcgggacgta
     2821 tcctgccatt atacgcctcc tcccgcgccg atggtccggc gcgaccgggc gcggcgcagg
     2881 ctgcctcgcc gctcaccagc cgatgccgta gtccnnnnnn nnnnccatag ttgctcgccc
     2941 cgccccacat cgtgccgtgc ttgcggtcga acatgatggc cgtgatcggg ccggaagtga
     3001 ggcgcgagaa cgtcagcgtg taccccatgg cctcgagctg cctccgcgcc tccggcggcg
     3061 tggactccgc cacgagcatc cgcccgggct cggagaggtg ctcgtcgaag ctgctgcgca
     3121 tctggaagct gttgatgttg ggcgcctcgc aggcctgctg cacgttcatg ccgaactcga
     3181 ccatgttcag gaagaactgc agcaggttct ggtcctggct gtcgccgcct tgcaccgaaa
     3241 acgccaggta cggctggccg tccttgagcg cgaggctcgg cgtcagggtg gaccgcggcc
     3301 gctggcccgg ccgcaccacg ttgaacggac agtcggcctc gtcgaggacg aacgcctgca
     3361 tgcgctggct caggccgacc ccggtcctgc cggcaacgac ggccggcacc cagccgccgc
     3421 tcggcgtcac cgacaccacc cagccctctt cgtcggcggc ctgcaccgag gtcgtgccgg
     3481 cgtagaagcc ctcgtcgtgg ctccgcaggc ccggctgctg gatgtcgtgc g
//
LOCUS       DAOSIF010000325         3516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23348_length_3516_cov_3.166137, whole genome shotgun sequence.
ACCESSION   DAOSIF010000325 DAOSIF010000000
VERSION     DAOSIF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23348_length_3516_cov_3.166137"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLT35_10475"
     CDS             complement(<1..193)
                     /locus_tag="PLT35_10475"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG29691.1"
                     /translation="MLRVALTGGIGTGKSVALARFAELGAAVIDADDLAHRAMQPGSE
                     GHAAIRRRFDDLRDEIADQR"
     gene            complement(201..1130)
                     /locus_tag="PLT35_10480"
     CDS             complement(201..1130)
                     /locus_tag="PLT35_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOG29692.1"
                     /translation="MAARILDGTAAAQAIRAELAPRVRAFTERAGRTPRLGIVLAGHD
                     PGSEIYVRNKLKTAGEAGIIAELVRIPEEAPVQEVLAAVAALNARRDADGILVQSPLP
                     EAMGADAETRVLDAIDPAKDVDGFTPANVGRLVQNRPGLVACTPAGIMELLSREQVAI
                     QGARAVVIGRSDIVGKPMALLLLHRHATVTICHSRTRDLPAVARTADILVAAIGRPGF
                     VTPEFVKPGAVVVDVGINSVSDEALVRRLLPPDSPRLAAFAKRGSLTVGDVHPSVSEV
                     AGALTPVPGGVGPLTIAMLLANTVAAAEARLRA"
     gene            complement(1208..2704)
                     /locus_tag="PLT35_10485"
     CDS             complement(1208..2704)
                     /locus_tag="PLT35_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG29693.1"
                     /translation="MSHLLATKSIEQLRAEAAESGEHSLKRVLGPVNLITLGIGAIIG
                     TGIFVLTGQAAAQYSGPAIVLSMLLAGLVSAFAGLCYAEFASSVPIAGSAYTYGYATL
                     GEFFAWIIGWDLILEYALGAATVAVGWSGYVVSFLHDFGIQFPAALSAAPGTVVTLAD
                     QTTVTAVLNLPAVLIALAVTLLLVIGIQESANVNSAIVIVKVAVVLLVIVAGAAFVNR
                     QHWVPFIPPNQGTFGAFGWSGIARAAGVIFFAYIGFDAVSTAAQEAKNPQRDMPIGIL
                     GSLAICTVLYVAVSLVMVGLVPYTQLGVPAPMAVAIDAAREQAAGTVWAGLVGVMPFL
                     VKLGAIAGLSSVMVVMMLGQPRIFYSMAHDGLLPAWAKKIHPRFRTPHITTIVTGCAV
                     AVAAGFTPINILGELVSIGTLMAFVIVSLGIIFLRRRRPDVVAPFRTPWVPVVPILSA
                     TVALLLMTSLPLPTWERLLVWMALGIAIYAGYGYRHSELRKRIEAGGR"
     gene            complement(2856..>3516)
                     /locus_tag="PLT35_10490"
     CDS             complement(2856..>3516)
                     /locus_tag="PLT35_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29694.1"
                     /translation="IIEEAPSPALSERARRKMGALVVQAAREVRYTNAGTFEFLLDAQ
                     GRFYFMEANTRLQVEHGVTELVTGVDLVKEQIRVAAGARLSFSQGDLSWRGHAIECRV
                     NAEDPETFAPSPGVISALSLPGGPGIRVDTALHADATVTPYYDSMVAKIMAHGRDRRE
                     ALARMARALDMTVVEGIKTSIPLQRRVLADPDFQAGRLSTAFMERFLPAPKPQRLAES
                     A"
BASE COUNT          547 a         1254 c         1233 g          482 t
ORIGIN      
        1 cgcgttggtc ggcgatctcg tcgcggaggt cgtcgaaccg ccgccggatc gccgcatggc
       61 cctccgaccc gggctgcatc gcgcggtgcg ccaggtcgtc cgcgtcaatg accgccgccc
      121 cgagctcggc gaacctggcg agggcgacgc tcttgcccgt cccgatgcct ccggtcagcg
      181 cgaccctcag catcggccgg tcaggccctc agccgggctt cggccgccgc gaccgtgttg
      241 gcgagcagca tcgcgatggt gagggggccg acgccgccgg ggaccggagt caaggccccg
      301 gccacctccg acacggaggg gtgcacgtct ccgacggtca gcgagccgcg cttcgcgaac
      361 gcggcgaggc gcggcgagtc gggcggaagc agccgcctga cgagcgcctc gtccgacacg
      421 gaattgatgc ccacgtccac caccaccgcg cccggcttga cgaactcagg cgtcacgaac
      481 ccggggcgcc cgatggcggc gaccaggatg tccgcggtgc gcgccacggc cggcagatcc
      541 cgcgtgcgcg aatggcagat cgtgacggtc gcgtggcggt gcaggagcag cagggccatg
      601 ggcttgccga cgatgtcgct gcgcccgatc accacggccc gggcgccctg gatcgccacc
      661 tgctctcgcg agaggagttc catgatgccg gccggcgtgc acgccacgag gccgggacgg
      721 ttctggacca gacggccgac gttggccggc gtgaacccgt ccacgtcctt cgccggatcg
      781 atcgcgtcga gaacgcgggt ttccgcgtca gcgcccatcg cctccggcag cggggactgg
      841 acgaggatgc cgtccgcgtc gcgcctggcg ttgagcgccg cgacggctgc gagcacctcc
      901 tggaccggcg cctcttcggg gatgcgcacc agctcggcga taatgcccgc ctctcctgcc
      961 gtcttcagct tgttcctgac gtagatctcg gaaccggggt cgtgccccgc gagcacgatg
     1021 cccaggcgcg gggtcctccc ggcccgttcc gtgaacgccc ggactcgagg agccagctcc
     1081 gcccggatcg cctgtgccgc cgcggttcca tccagtatgc gcgccgccat gtcacctccg
     1141 tgccgcaccg ggcgccttcg tcacggtccg gccgcgccgc ctgcggccgc ccgcgcgagg
     1201 gcgctgtcta ccggccgccg gcttcaatcc gcttccgcag ttcgctgtgc cggtacccgt
     1261 agccggcgta gatcgcaatg ccgagcgcca tccagacgag cagccgttcc cacgtcggca
     1321 gcggaaggct ggtcatgagc agcagggcga cggtcgccga gaggatcggc accaccggca
     1381 cccacggcgt tcggaacggc gccaccacgt ccggccgcct ccgcctgagg aagatgatgc
     1441 cgagcgacac gatgacgaac gccatgagcg tgccgatgct gaccagttcg ccgaggatgt
     1501 tgatcggcgt gaacccggcc gccaccgcca ccgcgcaccc ggtcacgatc gtcgtgatgt
     1561 gcggcgtgcg gaagcgcggg tggatcttct tggcccacgc cggcagcagg ccgtcgtgcg
     1621 ccatcgaata gaagatccga ggctgcccga gcatcatgac caccatcacc gagctgagcc
     1681 cggcaatggc gccaagcttg acgaggaagg gcatcacccc gaccaggccg gcccacaccg
     1741 tgccggccgc ctgctctcgg gccgcgtcga tggcgacggc catcggagcc ggcacgccga
     1801 gctgcgtgta gggcaccagt cccaccatga ccagcgagac cgccacgtag agcacggtgc
     1861 agatcgccag cgagccgagg atgccgatcg gcatgtcgcg ctgggggttc ttcgcctcct
     1921 gcgccgcggt ggacaccgcg tcgaacccga tgtaggcgaa aaagatcacg cccgccgcgc
     1981 gggcgatgcc gctccagccg aacgcgccga atgtcccctg attgggcgga atgaacggca
     2041 cccagtgctg gcggttgacg aacgccgccc cggcgacgat caccagcagc acgacggcca
     2101 ccttgacaat gacgatcgcc gagttgacgt tggccgattc ctggatgccg atcaccagca
     2161 gcagcgtgac cgccagcgcg atcagcaccg caggcaggtt cagcacggcg gtcaccgtcg
     2221 tctggtcggc gagggtgacg accgtgcccg gcgccgcgct cagggccgcg ggaaactgga
     2281 tcccgaagtc gtgaaggaag ctgacgacgt agcccgacca gccgacggcc acggtggccg
     2341 cgccgagggc gtactccagg atgagatccc agccgatgat ccaagcgaag aactccccga
     2401 gggtcgcgta gccgtaggta taggctgagc cggcaatcgg gacgctcgac gcgaactccg
     2461 cgtagcagag gccggcgaac gcgctgacca gcccggcgag cagcatcgac agcacgatgg
     2521 ccgggccgga gtactgggcg gccgcctgcc cggtgaggac gaagatgccg gtcccgatga
     2581 tcgcaccgat cccgagcgta atgaggttga ccgggccgag cacccgcttg aggctgtgtt
     2641 cgcccgactc ggcggcctcg gcgcgcagtt gttcgattga cttcgtggcg agcagatgag
     2701 acatcgagcg tcctccgccg ccgccggatc ggccgtcgtc gaggccgaca cgttcgagcg
     2761 gcggcccgat tcggcgtggc gtcggcatta tatgcgccgg gggggatcgg acactagagg
     2821 ggcgcgagcg cggcggcagg gccggaggcg cgcgctcagg cggactcggc gaggcgctgc
     2881 ggcttcgggg ccggcaggaa ccgctccatg aacgccgtcg agagccttcc cgcctggaag
     2941 tcgggatccg ccaggacccg gcgctgcagg ggaatcgagg tcttgatgcc ttcgacgacc
     3001 gtcatgtcga gggcgcgcgc catgcgcgcg agggcttcgc gccgatcccg cccgtgagcc
     3061 atgatcttcg cgaccatcga gtcgtagtac ggcgtcaccg tcgcgtcggc gtgcagggcc
     3121 gtgtccacgc gaatgcctgg ccccccgggc aggctcagcg ccgagatcac ccctggcgac
     3181 ggcgcgaacg tctcgggatc ctccgcgttc acgcggcact cgatcgcgtg gcctctccac
     3241 gacaggtcgc cctgcgagaa cgagagccgc gcgccggcgg cgacgcggat ctgctccttc
     3301 acgaggtcca cgccggtcac cagctctgtc acgccgtgct cgacctggag ccgggtgttg
     3361 gcctccatga agtagaagcg cccctgcgcg tccaggagga actcgaaggt cccggcgttg
     3421 gtgtagcgga cctcgcgggc cgcctgcacg acgagggccc ccatcttcct gcgggcgcgc
     3481 tcgctgagcg cgggcgacgg cgcctcctcg atgatc
//
LOCUS       DAOSIF010000326         3503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23427_length_3503_cov_3.685615, whole genome shotgun sequence.
ACCESSION   DAOSIF010000326 DAOSIF010000000
VERSION     DAOSIF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23427_length_3503_cov_3.685615"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PLT35_10495"
     CDS             complement(<1..1147)
                     /locus_tag="PLT35_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29695.1"
                     /translation="MRRGAAARAALFACALALTCAGPASAQEGVFAGLTKGIESVFSS
                     VSTTTTFASGTVTKTDTTTTYPALTLTLDTRIFPALALNAGGVFEVNFMSVGTGGART
                     DSTVTRNRPFFLLRSTNPVFAPGVGYFRREDRSRTAGLSNVKLVNDEFAGYLLWNPVG
                     GPRSDFQFLRTHTFDEDRAIQDVTKDFGTLVSNYAYGGLGAHYRGAYLRTDDARHALE
                     TTQIVHGGRVDYSAALLAKRLLWNATYNVNSHDLRTDARGSGGEVEFPLTPYRALAAQ
                     SDTPATAKLTEHAALADGNLTAGAGVDLGVPATPLDAQARNIGLDFLNPTQVNRVLVW
                     VDRSLPIEVANAFSWEIYSSPDNIIWTRESAVPVAPFGAFENRFEIDF"
     gene            complement(1148..2521)
                     /locus_tag="PLT35_10500"
     CDS             complement(1148..2521)
                     /locus_tag="PLT35_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOG29696.1"
                     /translation="MSPLRVALFLIGLLIPALASAADGGIHLYLQPLASSASRLTLSL
                     ASVSAVAASGSEHPIALRLKDIGAAAGRQRLLASGRLPAGAYAGFSIQAGRAALQDGP
                     VEVALAVPEAPVRLDFPFVVSDRQAVLVWLTLKYDESLTNGAAFSPVFSAVAPPRPIA
                     ELAGFVTNGGSNTMTVFDKRLGQAVGVIETCAGPSGMALDQRRRRAYVACPAADEIQS
                     VDVATASVLERTRVSPGDRPRELALTPDGLTLVSANTGSNSISFFDAATLTRQERINV
                     GSGPGSLLVDPSGKRAFVFNTLSSSVSVIDLAARSLAATLSTDLAPLRGAFSRRGDRL
                     FVIHERSPYLTVLDARQLGVAAKARLGAGDDAVAVDPVRDLVCVAGSRDTVVEFYDPN
                     TLLPLYTMRTQSGVAHLAVDAESSALYMVSPDRRSLVIGRLADRKIASEIDVGDDPRW
                     VAVMGEK"
     gene            complement(2693..>3503)
                     /locus_tag="PLT35_10505"
     CDS             complement(2693..>3503)
                     /locus_tag="PLT35_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29697.1"
                     /translation="TFPSSQLACTSCHDPHGRVRRTSSGTYTTGTTEPVSGSGSYGAI
                     PAAGFTVGNYRLLRGLNDSHVTGVTFTGVAIAVAPSTYNRTEASTQTRVAYGASSINT
                     WGNWCASCHPGMHSSGNYVHPVDQSLGSNLALVYNSYVKSGDLTGSSATAYTSLVPFA
                     ENTGDIATLAAHAKIDNSQLGGPGASDKVMCLSCHRAHASGFEYALRWNMEGEFMIWN
                     SLYPGTDTTPSVPQFARGRLGAETQAAYYDRPVTAFAAYQRVLCNKCHAKD"
BASE COUNT          533 a         1190 c         1266 g          514 t
ORIGIN      
        1 ggaagtcgat ctcgaagcgg ttctcgaacg cgccgaacgg cgccaccggc accgccgatt
       61 cgcgcgtcca gatgatgttg tccgggctgc tgtagatctc ccaggagaag gcgttggcga
      121 cctcgatcgg cagggagcga tcgacccaga ccaggacgcg gttcacctgc gtcgggttca
      181 ggaagtccag gccgatgttc ctcgcctggg cgtccagcgg cgtcgcgggt acgccgaggt
      241 ccacgccggc tcccgccgtg aggttgccgt cggcgagggc ggcgtgctcc gtcagcttcg
      301 ccgtcgccgg cgtgtcgctc tgtgcggcga gcgcccggta cggcgtcagg gggaactcga
      361 cttcgccgcc gcttcctctc gcgtccgtcc tgagatcgtg ggagttgaca ttgtaggtcg
      421 cgttccacag cagccgcttg gcgagcagcg cggccgagta gtccacgcgg ccgccgtgaa
      481 cgatctgcgt cgtctcaagc gcgtgccgcg catcgtcggt ccggaggtac gcgccgcggt
      541 agtgcgcgcc gagccccccg tacgcgtagt tggagacgag cgtgccgaag tccttcgtca
      601 cgtcctggat cgcccggtcc tcgtcgaatg tgtgcgtcct gaggaactgg aaatccgagc
      661 ggggcccgcc cacgggattc cacaggaggt acccggcgaa ctcgtcgttg accagcttga
      721 cgttcgagag gcccgcggtt cgcgagcggt cttcccgccg gaagtagccg acccccggcg
      781 cgaacaccgg gtttgtcgag cggagcagga agaacggccg gttccgcgtg accgtcgaat
      841 ccgtcctggc gccgccggtc ccgaccgaca tgaagttgac ctcgaacacg ccgcccgcgt
      901 tcagcgcgag ggccggaaag atccgcgtgt ccagggtgag cgtcagcgcc gggtacgtag
      961 tcgtcgtgtc cgtcttcgtg accgtcccgg acgcgaacgt cgtcgtggtg gacacggagc
     1021 tgaagaccga ctcgatgccc ttcgtcaggc cggcaaacac gccctcttgc gcggaggccg
     1081 gcccggcgca ggtgagggcc agcgcgcagg cgaagagcgc ggccctggcg gcggctcccc
     1141 ggcgcaccta cttctccccc atcacggcca cccagcgcgg gtcgtcgccg acgtcgatct
     1201 cggacgcgat cttcctgtcc gcgagccggc cgatgaccag gctccggcga tcggggctga
     1261 ccatgtagag cgcgctgctc tcggcgtcga cggcgaggtg cgcgacgccc gactgcgtgc
     1321 gcatggtgta cagcggcagc agcgtgttgg gatcgtagaa ctcgacgacc gtgtcgcggc
     1381 tgccggcgac gcacacgaga tcgcgcaccg ggtcgaccgc gacggcgtcg tcgccggccc
     1441 cgaggcgcgc tttcgcggcg acgcccagct gccgagcgtc gagcacggtc aggtagggcg
     1501 accgctcgtg gatcacgaag agccggtcgc cgcgccggct gaatgccccc ctgagcgggg
     1561 ccaggtccgt cgagagcgtc gccgcgaggc tccgggccgc caggtcgatc accgaaacgg
     1621 agctcgacag cgtgttgaag acgaaggccc gcttccccga cggatcgacg agcagcgaac
     1681 ccggcccgct tcccacgttg atgcgctcct gccgcgtcag cgtggccgcg tcgaagaagc
     1741 tgatgctgtt cgatcccgtg ttggccgaga ccagcgtcag cccgtccggc gtcagcgcca
     1801 gctcgcgcgg ccggtcgccg ggagacacac gggtccgctc gaggaccgag gccgtcgcca
     1861 cgtccaccga ctggatctcg tcggccgccg ggcacgccac atacgcccgc cggcggcgct
     1921 ggtcgagggc catcccggac ggacccgcgc aggtctcgat gaccccgacc gcctggccaa
     1981 gccgcttgtc gaacaccgtc atcgtgttcg acccgccgtt tgtgacgaag ccggccagct
     2041 cggcaatcgg cctgggcggc gcgacggccg agaacacggg gctgaacgcc gcgccgttcg
     2101 tcaacgactc gtcgtacttc agggtgagcc agacgagcac ggcctgccga tccgacacga
     2161 cgaaggggaa atcgagcctg accggcgcct ccggcacggc cagcgcgacc tcgacggggc
     2221 cgtcttgcag ggcggcccgc ccggcctgga tgctgaagcc tgcgtaggcg cccgccggga
     2281 gccggccgga cgccaggagc cgctgccgtc cggcggcggc gccgatgtcc ttcaggcgca
     2341 gcgcaatcgg gtgttccgaa cccgacgccg caacggcgga gaccgacgcg agcgagaggg
     2401 tcagcctcga ggcgctggag gccagcggtt ggagatagag atggattcct ccgtcagccg
     2461 cgctggcgag agcggggatc aggagtccga tgaggaagag cgccacgcgc agtggggaca
     2521 cggcctcgtc gccagggaga cggcgccgcg cggcgatcat ccccgcacgc acagggaact
     2581 cccgccggac cgttccaggg tcccggttgc cggacggcac gcccgcgaga gaagagggct
     2641 gggttcgagg cccagccctc cacaccacat cacatgacgg cttgagcccg atctagtcct
     2701 tcgcgtggca cttgttgcag agcacgcgct ggtacgcggc aaacgcggtc acgggccgat
     2761 cgtagtacgc cgcctgggtc tctgccccga ggcgcccccg ggcgaactgg ggaaccgacg
     2821 gcgtcgtgtc cgtgccgggg tagaggctgt tccagatcat gaactcgcct tccatgttcc
     2881 agcgcagggc gtattcgaag ccgctcgcgt gcgcgcggtg gcaggacagg cacatgacct
     2941 tgtcgctggc acccggcccg cccagctgcg agttgtcgat cttcgcgtgg gccgcgaggg
     3001 tcgcgatgtc gcccgtgttc tcggcaaacg gcaccagcga cgtgtaggcg gtggccgacg
     3061 agccggtcag atcgccggac ttgacgtacg agttgtagac cagggccagg ttcgacccga
     3121 ggctctgatc gaccgggtgc acgtagttgc cgctggagtg catgccgggg tggcacgagg
     3181 cgcaccagtt gccccacgtg ttgatcgagc tggcgccgta ggccacgcgg gtctgcgtcg
     3241 aggcttcggt ccggttgtac gtggacggcg cgacagcaat cgcgacgccc gtgaaggtga
     3301 cgccagtcac gtgggagtcg ttcaggccgc gcaggaggcg gtagttgccc acggtgaagc
     3361 cggctgccgg aatcgcgccg tacgagccgg agccgctgac cggctcggtc gtcccggtcg
     3421 tgtaggtccc cgacgaggtg cggcggacgc ggccgtgcgg gtcgtggcag ctggtgcacg
     3481 cgagctggga cgacgggaac gtg
//
LOCUS       DAOSIF010000327         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23507_length_3492_cov_3.967704, whole genome shotgun sequence.
ACCESSION   DAOSIF010000327 DAOSIF010000000
VERSION     DAOSIF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23507_length_3492_cov_3.967704"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLT35_10510"
     CDS             <1..1205
                     /locus_tag="PLT35_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HOG29698.1"
                     /translation="RVRVTNRYDFAGLEGLAVSWRLLEDGRPVQSGSLPGPLDVRPGE
                     SREIALPYDARRVGPGREGAVEVTFSLREAAPWAPAGHEVAWEQLALPARTREDTGAA
                     KAGAAGPAAMPPLKVASDGGRVTLSGPGFEAAIEGGALVSYVWRGQELLAGPLVPNLW
                     RVPTDNDEGGGRRSYAHRWREAGLDRLAVTAGEPQVLQVDPGRARVTVERRLQGASAA
                     MLLTTVYDVAGDGEIAIAAAFRAEGSFPPLPRVGFQVQLPGRFDAVEWYGRGPQESYP
                     DRKEGARLGVHRASIAGLHFPHVMAQENGSRTDVRWAAFTGPSGAGLHVSGDPLFAFT
                     AHDYTDAALTAAKTSQAIERDGRVTLSLDLAQMGLGGDDSWSPRVHAEYLLDAAEYRF
                     AFRLRGIR"
     gene            1362..3224
                     /locus_tag="PLT35_10515"
     CDS             1362..3224
                     /locus_tag="PLT35_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29699.1"
                     /translation="MSTTSESRSDAFLRIAGQFRLGALTTESSHPVTADLSETAGRDT
                     AAALKLLFDVDDDVVRTYRAFVGSGRAAGIAGTLSRALRRGGRVFFTGCGSTGRLSIQ
                     LVSIWREFWQRQQARGLACEPPAAAWEDRAFSVMAGGDFALIKAVEGFEDFTDFGRKQ
                     IGDLGVCDRDVVFAITEGGETSFVIGTAWKGVEAGSKVYFVYNNPDEILCAHVTRSRE
                     VIEDPRIEKINLTTGPMAITGSTRMQATTIQLCVLLTVMEMVLRDLMADLEPGGPCAL
                     DPAVVPRAFLAELVEMHARLKDPALLDRLAALVTLEEATYLAGRRHTYYADRYGIDVL
                     TDTTERSPTYCTPPFRKFDDATAAESWAFLFVPAATTPEAWVHLIKREPACVEWADDE
                     VRSLVAPDKLQQTLETVRKIGRRELMRFKVGLDGLPYRRLGAGDSAVAIVGDADAAAL
                     VEAGGFHRERLEEARAAGARTGVIAVGRGAWLERLQEGCADAACVTLAVRVPDSDFLL
                     DGVARAGLKMLLNALSTCTMVRLGRVMGNYMVWLVASNLKLIDRATRYIEQLAGLDYA
                     QANRLLFEVIEYVEPRWKADQAYPPVVGLAVTRAQRGLGNEEAEAALAATSASR"
     gene            complement(3205..>3492)
                     /locus_tag="PLT35_10520"
     CDS             complement(3205..>3492)
                     /locus_tag="PLT35_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29700.1"
                     /translation="TWVFEVQTQAGSGAPQVTFKQDGEALTGHYSSSLLGEAELKGTV
                     KGRVITFTMTLTVQGTSLDVTYTGTIEDSNSMKGTVDFAGMGEGTFTGKRK"
BASE COUNT          453 a         1219 c         1308 g          512 t
ORIGIN      
        1 gccgggtgcg tgtcacgaac cgctacgact tcgccggcct cgaggggctg gcggtctcgt
       61 ggcgcctgct cgaggacggc aggcccgtgc agtcggggtc gctgcccggc ccgctggacg
      121 tgcggcccgg ggagtcgcgg gagatcgcgc tgccgtacga cgcccgcagg gtcgggcccg
      181 gacgcgaagg cgcggtcgag gtgaccttct cgcttcgcga ggccgcgccg tgggcgccgg
      241 ccgggcacga ggtggcgtgg gagcagctgg cgctgcccgc gcggacgcgt gaggacaccg
      301 gggcggcgaa ggccggcgcg gccggcccgg cagccatgcc gcctctgaag gtcgcctcgg
      361 atggcgggcg cgtcacgctg tcggggcccg gcttcgaggc ggccatcgag ggcggggccc
      421 tggtctccta cgtctggcgc ggacaggagc tgctggccgg gccgctcgtg ccgaacctct
      481 ggcgcgtgcc cacggacaac gacgagggcg gaggccgccg gagctacgcc cacaggtggc
      541 gggaggccgg cctcgaccgc ctcgccgtca ccgcgggcga gccgcaggtg ctccaggtcg
      601 atccgggccg ggcgcgcgtg acggtcgagc gccggctgca gggcgcgtcg gccgcgatgc
      661 tgctcacgac cgtctacgac gtggccggcg acggcgagat tgccatcgcg gcggcgttcc
      721 gcgccgaggg atcgtttccg ccgctgccga gggtggggtt ccaggtgcag ctgccggggc
      781 ggttcgatgc cgtcgagtgg tacggccgcg gcccgcagga gagctacccc gaccgcaagg
      841 aaggggcgcg cctcggcgtc caccgcgcga gcatcgccgg cctgcacttc ccgcacgtga
      901 tggcgcagga gaacggcagc cgcaccgacg tccggtgggc cgcgttcacg ggcccgtccg
      961 gcgccggcct ccacgtgtcg ggcgacccgc tgttcgcctt cacggcgcac gactacacgg
     1021 acgcggcgct cacggccgcc aagactagcc aggcgatcga gcgggacggc cgcgtgacgc
     1081 tgagcctcga cctggcgcag atgggccttg gcggcgacga cagctggtcg ccgcgcgtgc
     1141 acgccgagta cctgctcgac gcggccgagt accggttcgc cttccggctc cgcggcatcc
     1201 gctgatcccg gggcctcgca ggcgtccgtg gtaacgtgtg cacgttcgtc gcgcggggcg
     1261 cggcgtttgt cgcgcggcgt tcggcgtgcg agaggcggcg cgggtcgtgc gacgggcggc
     1321 gatcgccgtc tgtgttctga cttctgactt cttcttctgc catgagcacc acttccgaat
     1381 cccgctccga cgccttcctg cgcatcgccg gccagttccg gctgggcgcg ctgacgaccg
     1441 agtcctccca cccggtgacg gcggacctga gcgagacggc gggccgcgac acggccgcgg
     1501 ccctgaagct cctcttcgac gtcgacgacg acgtcgtccg cacgtaccgg gcgtttgtcg
     1561 ggtccgggcg cgcagccggg atcgccggga cgctctcgcg ggcgctccgc cgcggcggcc
     1621 gcgtcttctt cacgggctgc ggatcgaccg gccgcctcag cattcagctg gtgtcgatct
     1681 ggcgcgagtt ctggcagcgc cagcaggcgc ggggcctcgc gtgcgagcct ccggcggcgg
     1741 cgtgggagga tcgcgcgttc agcgtgatgg ccggcggcga tttcgcgctg atcaaggccg
     1801 tcgagggctt cgaggacttc accgacttcg gcaggaaaca gatcggcgac ctcggcgtct
     1861 gcgatcgcga tgtcgtcttc gccatcaccg aaggcggcga gacctcgttc gtgatcggca
     1921 cggcgtggaa gggcgtcgag gcgggctcga aggtctactt cgtctacaac aacccggacg
     1981 agatcctctg cgcgcacgtc acccgcagcc gcgaggtcat cgaggacccg cggatcgaga
     2041 agatcaacct gacgaccggc ccgatggcca tcaccggctc gacgcggatg caggccacga
     2101 cgatccagtt gtgcgtcctg ctgacggtga tggagatggt gctgcgcgac ctgatggccg
     2161 atctcgagcc cggcggcccg tgcgcgctcg atccggccgt cgtcccccgc gcgttcctcg
     2221 ccgagctggt cgagatgcac gcgcggctga aggacccggc gctcctggac cggctggcgg
     2281 ccctcgtgac gctcgaggag gcgacgtacc tggccggccg caggcacacc tactacgcgg
     2341 accggtacgg catcgacgtc ctgaccgaca cgacggagcg ctcccccacc tactgcacgc
     2401 cgccgttccg gaagttcgac gacgccacgg ccgccgaatc gtgggccttt ctcttcgtgc
     2461 cggccgcgac gacgccggag gcgtgggtcc accttatcaa gcgcgagccg gcctgcgtcg
     2521 agtgggccga cgacgaggtc cgctcgctcg tcgccccgga caagctgcag cagacgctcg
     2581 agacggtccg gaagatcggc cggcgggagc tcatgcggtt caaggtgggc ctcgacggcc
     2641 tgccctacag gcgcctcggc gccggcgaca gcgccgtcgc catcgtcggc gacgccgatg
     2701 cggcggcgct cgtggaggcg ggcgggttcc accgggagcg gctggaggag gcgcgcgcgg
     2761 cgggcgcccg cacgggcgtc atcgccgtcg ggcgcggagc ctggctggag cggctgcagg
     2821 agggatgtgc ggacgccgcg tgcgtcacgc tggcggtgcg cgtgcccgat tcggacttcc
     2881 tgctggacgg ggtcgcgcgc gcgggcctga agatgctgct caacgcgctg tccacgtgca
     2941 cgatggtcag gctcggccgc gtgatgggca actacatggt gtggctcgtg gcgagcaacc
     3001 tgaagctcat cgaccgggcg acgcggtaca tcgagcagct tgcggggctc gactacgcgc
     3061 aggccaaccg cctgctcttc gaggtcatcg agtacgtcga gccgcgctgg aaggccgacc
     3121 aggcctaccc gcccgtcgtc ggcctggcgg tgacgcgggc gcagcgcggc ctcggcaacg
     3181 aggaggccga agcggcgctg gccgctactt ccgcttcccg gtgaacgtcc cctcgcccat
     3241 gccggcgaag tcgaccgttc ccttcatgga gttcgagtct tcgatcgtgc cggtgtaggt
     3301 cacatcgagc gaggtgccct ggaccgtgag cgtcatggtg aacgtgatca cccggccttt
     3361 caccgtgccc ttgagctcgg cctcgccgag gagcgacgac gagtagtggc cggtgagcgc
     3421 ttcgccgtcc tgcttgaagg tgacctgcgg cgcgcctgat cccgcctgcg tctgcacctc
     3481 gaagacccac gt
//
LOCUS       DAOSIF010000328         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23508_length_3492_cov_3.474251, whole genome shotgun sequence.
ACCESSION   DAOSIF010000328 DAOSIF010000000
VERSION     DAOSIF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23508_length_3492_cov_3.474251"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLT35_10525"
     CDS             <1..839
                     /locus_tag="PLT35_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG29701.1"
                     /translation="GQGPGRPGWHLECSAMALRLLGEPPIDLHAGGVDLIFPHHENEI
                     AQSEGATGRPFSRFWVHVEHLLLDEEKMSKSLGNVFTVPDVAKRGHHPSALRYLFIST
                     HYRKQLKFSWAGLQQAEEAVQRLMDFLARVERVEGEGAHPEVQRRVAAGRAEFGAMLE
                     EDLNTPGALGVVFELVRSLNSAVDAGQVGKPDANVIRAAFDDIDRILGIVALRRQEDA
                     AAAVPTDEIERAIAERAAARRRRDFAAADRIRRQLADSGIVLEDSPTGTRWKLAGRGG
                     RA"
     gene            complement(967..2205)
                     /locus_tag="PLT35_10530"
     CDS             complement(967..2205)
                     /locus_tag="PLT35_10530"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOG29702.1"
                     /translation="MKILVFGGGGKMGTTVAFDLLKHDAVATVGLADRSHGALENARA
                     WLKSPKVATHQVDVTKRDDLTALMKHYDVGVSTLPDRRTSYIVVDAAVRCGFDFVDIL
                     EEYHRRPDAYEVEGLVIPPGMGLNEYGDWLHDTAQKNGVTFLDGIGFAPGMSNVTCGE
                     GIRKLDQAESVIARVGGIPNKEAAARHPLRYMITWAFSHVLREYVIKVCILQDGKIVE
                     VDASSGREAFSFDKFGKSERLECAITPGMPSFVYTRPQLKEFAEKTVRWPGHWDGVQT
                     LKECGMLDLDPVEVGGVKVVPRDVLLARIEPRLRALPGETDVCVMYNTVEGVKDGRRT
                     RISYHLWDEADTVNSVSSMGRVTGYSAAIGAVMVGSGLIAETGIVAPEDAIYGANYAH
                     FIGELAKRNIRILETIETIG"
     gene            2377..2766
                     /locus_tag="PLT35_10535"
     CDS             2377..2766
                     /locus_tag="PLT35_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG29703.1"
                     /translation="MGSDATVALGITGESLACAALAERGYAILARRYRTRNGEIDIVA
                     RDGPTLVFVEVKCRASGACGHPAEAVTLRKQRRVVAMARRYLAERGLHGCLCRFDVVA
                     VRFRGERPPAVEVIRDAFQAGWWAPPP"
     gene            2809..2883
                     /locus_tag="PLT35_10540"
     tRNA            2809..2883
                     /locus_tag="PLT35_10540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2841..2843,aa:Gly,seq:gcc)
     gene            2930..3004
                     /locus_tag="PLT35_10545"
     tRNA            2930..3004
                     /locus_tag="PLT35_10545"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2962..2964,aa:Cys,seq:gca)
     gene            3093..>3492
                     /locus_tag="PLT35_10550"
     CDS             3093..>3492
                     /locus_tag="PLT35_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG29704.1"
                     /translation="MTLLRDILDAAYTPVARAMSGVHPNTLTLVSVASGAAAGIAFAA
                     AGGGRGWYAAAGLLVALSGSADALDGLVARLYGRVSAGGDFLDHAGDRFVEVAILAGI
                     AASPGAHTVFGLSAVILTLLNSYLGTQIEAT"
BASE COUNT          508 a         1228 c         1193 g          563 t
ORIGIN      
        1 acggccaggg cccagggcgg cccggctggc acctcgagtg ctccgcgatg gcgctgcgcc
       61 tgctgggcga gccccccatc gacctgcacg ccggcggcgt ggacctcatc ttcccgcacc
      121 acgagaacga gattgcccag agcgaaggtg cgaccggacg cccgttttcg cggttctggg
      181 tccacgtcga gcacctcttg ctcgacgaag agaaaatgtc caagtcgctg ggcaacgtgt
      241 tcaccgtgcc ggacgtggcc aagcgcggcc accatccgtc ggccctccgc tacctgttca
      301 tctccacgca ctatcgcaag cagctcaagt tctcgtgggc gggcctgcag caggccgagg
      361 aggccgtgca gcgcctgatg gatttcctgg cgcgcgtcga gcgcgtcgaa ggcgaaggcg
      421 cgcacccgga ggttcaacgc cgcgtggcgg ccggccgggc ggagttcggg gcgatgctcg
      481 aggaggactt gaacacgccc ggcgcgctcg gtgtggtgtt cgaactcgtg cggtcgctga
      541 acagcgccgt cgacgccggg caggtgggga agccggacgc gaatgtcatc cgggcggcct
      601 tcgacgacat cgaccgcatc ctcggaatcg tggcgctgcg ccgccaggaa gacgcggcgg
      661 ccgccgttcc gaccgacgag atcgagcgcg cgattgccga gcgggcggcc gcgcggcgcc
      721 ggcgcgactt cgccgccgcc gatcgcatca ggcggcagct cgccgatagc ggcatcgtcc
      781 tcgaggacag cccgacgggc acgcgctgga agctcgccgg caggggcggg agagcgtagg
      841 gagagaattc aggagtcaga agccagaaac cagaagtcag attccggaat gcggagcgcc
      901 ccgttccccg gcccctgatc ccccacctcc gagtcccgaa cccccaatcc cgaacccggg
      961 ctgtctctac ccgatcgtct cgatcgtctc gaggatccgg atgttccgct tcgcgagctc
     1021 gccgatgaag tgcgcgtagt tcgcgccgta gatcgcatcc tccggcgcga cgatgcccgt
     1081 ctcggcgatc aggccgctcc cgaccatcac cgcgccgatc gcggcgctgt acccagtgac
     1141 ccggcccatg gacgacacgc tgttgaccgt atcggcctcg tcccagaggt ggtaggagat
     1201 gcgggtgcgc ctgccgtcct tgacgccctc gacggtgttg tacatcacgc acacgtccgt
     1261 ctcgccagga agcgcgcgca ggcgcggctc gatgcgggcg aggagcacgt cgcgcggcac
     1321 gaccttgacg ccgcccactt ccaccgggtc gaggtccagc atgccgcact ccttgagcgt
     1381 ctggacgccg tcccagtgcc cgggccagcg gaccgtcttc tcggcgaact ccttcagctg
     1441 cgggcgcgtg taaacgaagc tcggcatgcc gggcgtgatg gcgcactcga gccgctcgga
     1501 cttgccgaac ttgtcgaacg aaaacgcctc gcggcccgac gaggcgtcca cctccacgat
     1561 ctttccgtcc tgcaggatgc acaccttgat gacgtactcg cggagcacgt gggagaaggc
     1621 ccaggtgatc atgtagcgca gcgggtggcg cgcggcggcc tctttgttcg ggatgccgcc
     1681 gacgcgggcg atgaccgact ccgcctggtc cagcttccgg atgccctcgc cgcacgtcac
     1741 gttgctcatg cccggcgcga agccgatccc gtccaggaag gtgacgccgt tcttctgcgc
     1801 cgtgtcgtgg agccagtcgc cgtactcgtt caggcccatt ccgggcggga tcacgagccc
     1861 ttcgacctcg tacgcgtccg gccggcggtg gtactcctcc aggatgtcca cgaagtcgaa
     1921 cccgcagcgc acggcggcgt ccacgacgat gtagctcgtc cgcctgtccg ggagcgtact
     1981 gacgccgacg tcgtagtgct tcatgagcgc cgtcagatcg tcccttttgg tgacgtccac
     2041 ctggtgcgtg gcgaccttgg ggctcttcag ccacgcgcgg gcgttttcca gcgcgccgtg
     2101 gctccggtcg gccagcccga ctgtggcgac ggcatcgtgc ttcaggagat cgaacgccac
     2161 cgtcgtgccc atcttgccgc cgccgccaaa gaccaggatc ttcatgaggg actccctgtg
     2221 ccgcgcacgc gcgcgccgaa ccgcccgtcc acccggaggg gccgtgctga gggggcgcga
     2281 gctgccacac cggcgccgcg cgcgccgggc gcaggactgc acattataac gtagcgccgg
     2341 cgggcgcctg gcccccgcct tgcttgccgt ggcggcatgg gatcggacgc caccgtcgcg
     2401 ctcggaatca ccggggaatc tctggcctgc gcggccttgg ccgagcgggg gtacgcgatt
     2461 cttgcccgcc ggtaccgcac gcggaacgga gaaatcgaca tcgtggcacg cgacgggccg
     2521 accctcgtct tcgtcgaggt gaagtgccgg gcctccggcg cgtgcgggca tccagccgag
     2581 gccgtgacgc tgcggaagca gcgccgggtg gtggcgatgg cgcgcaggta tctggccgag
     2641 cgcggccttc acggctgcct gtgccgattc gacgtcgtcg cggtgcggtt caggggggag
     2701 cggccgccgg ccgtcgaggt gatccgcgac gcgtttcagg ccggctggtg ggcgccgccg
     2761 ccttgagtcg tttcgcgcgc ctgtgctaac gtaggtgcag gcggtcgggc gggaataact
     2821 cagtggtaga gtgcgacctt gccaaggtcg aagtcgcggg ttcgaatccc gtttcccgct
     2881 ccatctcctt ccttccagga acggccggac gcgtggcgat tcccgacagg gcgccgtagc
     2941 caagtggtaa ggcagaggtc tgcaaaacct ccattctccg gttcaaatcc ggacggcgcc
     3001 tccaccccct ccgtcgaatc cgaggctccc ctgagagtgc cggttgccgc cagcggagag
     3061 tacggtaaga tgcccgcgcc tttcgcgcat tcatgacgct tctccgcgac atcctcgacg
     3121 ccgcctacac ccccgtcgcc agggccatgt ccggcgtcca cccgaacacg ctgacgctcg
     3181 tgtcggtggc gtcgggggcc gctgccggga tcgccttcgc tgccgccggc ggcgggcgcg
     3241 gctggtacgc ggcggccggc ctgctcgtcg cgctctcggg ctcggccgac gccctggacg
     3301 ggctcgtggc ccggctctac gggcgggtgt cggcaggcgg cgacttcctg gatcacgcgg
     3361 gcgaccgttt cgtcgaggtg gccattctcg ccggcatcgc cgcgtcgcca ggcgcccaca
     3421 cggtcttcgg cctgtcggcg gtgatcctca ccctgttgaa cagctacctc ggcacccaga
     3481 tcgaagccac gt
//
LOCUS       DAOSIF010000329         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23511_length_3492_cov_3.223742, whole genome shotgun sequence.
ACCESSION   DAOSIF010000329 DAOSIF010000000
VERSION     DAOSIF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23511_length_3492_cov_3.223742"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLT35_10555"
     CDS             <1..564
                     /locus_tag="PLT35_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG29705.1"
                     /translation="RRAPGMKLAFIGTHGTGKPTLCYDLTAGQKRQGLNADMVKEVAR
                     LSPLPINRKTSLDAQTWILMTQVAEEIRSASAHDIVVCDRSVLDNYAYLVFACGRQRA
                     IERFVDHWMHTYSVLVKVPMLAPAAEDGVRDTDEFFVRSIDRLVDALLEEKRIAHLRL
                     DAGSREGWVATALRAVTDHPSLAGRLL"
     gene            706..2250
                     /locus_tag="PLT35_10560"
     CDS             706..2250
                     /locus_tag="PLT35_10560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG29706.1"
                     /translation="MRLRLAAGLGLLLASMCWPAPAAAQEVSLAGQVVERILPNGLKV
                     LMVKRTEAPLIRCILAYRVGAVNERPGITGISHFHEHMMFKGTRSMGIKPGTFARDEE
                     YNRQIDALMDQVAAEEARPGARDEAKIAGLKQQASALLERQKKETIVSEEIWSAYQAA
                     GGTGINASTGSEMTQYYVTLPKNTLELYLALEADRMAHPVFREFYSERDVVAEERRMS
                     ENSPGFFFQEQLNATFYAAHPYANPVVGWMSDISRVTKQEMIAYREIYYRPDNATLVL
                     AGDLDPEQTMALVEKHFGGIASRGPSPRVRTEEPSPFYYRRTIGGSFKPPFIEKRVIG
                     RAATNPQVVLMFHIPPLWHDDLPALYMLGRVLGARTGRMYLDLVQAKEHATSVMASAS
                     DSMYDGVFRVSATGREVQGELAAPLDQIEKGLWSFIEDAKSVPVDPQLLERTKNSVEA
                     QYLQGLAGTGIAGTLARMETAYRWQYIDEQYRRRMAVTPDDLMRVAKAYLTRDNCVTG
                     ILEREQ"
     gene            2279..>3492
                     /locus_tag="PLT35_10565"
     CDS             2279..>3492
                     /locus_tag="PLT35_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG29707.1"
                     /translation="MLKRLSAVLLVGAFVTAAAAAAQQTAVKPRKTPGAAAVAPERAE
                     QGPRPEHFTFPPLAFTPPKAADYRAQLSNGLVVYLAEDHAIPWFEAMLLTPVAGGAAG
                     FGRGRGAGPDEGAPDLPGRLRLSGRGGSWPQGRGGGGGGPKSFLEPADKLGIQDLTAS
                     VMRTGGTTSMSADQINERMEFLAGSVSPTSLSIHMRHLDEGLRIWLDILTSPAFPEDR
                     LRRERQAMTAPLRNRNRNLTAVASDTWGKLIYGEASPVTAEATEATINSITRDDVVAW
                     HRKYWGANNAILVAAGDFTKAEMLRRLEATFGKWRASEAKAAPAYPVVPSAAAAPGVY
                     MVQPLGVTPNQGIIRVGTLSITQDDPDYAAVDLMNYTLGGGSFSSRITQIVRNDYGLA
                     YTANSSVGAELH"
BASE COUNT          572 a         1188 c         1236 g          496 t
ORIGIN      
        1 cgccgggctc cgggcatgaa gctggcgttc atcggcacgc acgggacggg caagccgacg
       61 ctctgctacg acctcacggc ggggcagaag cgccagggcc tgaacgcgga catggtgaag
      121 gaagtcgcgc gcctctcgcc gctgccgatc aaccggaaga cctcgctcga cgcgcaaacc
      181 tggatcctca tgacgcaggt ggccgaggag atccgctccg ccagcgccca cgacatcgtc
      241 gtctgcgacc gcagcgtgct cgacaactac gcgtacctcg tgttcgcctg cgggcgccag
      301 cgcgccatcg agcgcttcgt ggaccactgg atgcacacgt acagtgtgct cgtcaaggtg
      361 ccgatgctgg cgccggccgc cgaggacggg gtgcgcgaca ccgacgagtt cttcgtgcgc
      421 tccatcgatc ggctggtgga cgccctgctc gaggagaagc gcatcgccca cctccgcctt
      481 gatgccggca gccgggaggg ctgggtcgcg acggccctgc gggcggtcac ggatcaccca
      541 agcctggccg gacggctctt gtaggcgcgg cgcgagccgc gttctcgtgc agcccggcgc
      601 cctccgggcg cggcctgggg cccgccgtgc tatgctgtgg cgccgccggg gcggatctgg
      661 ggccccggca ggcggccgtc caccctcgag catggagaac cgaacatgag acttcgactc
      721 gctgcgggtc tcggcctgct gctggcgtcg atgtgctggc ccgcgcccgc ggcggcgcag
      781 gaggtcagcc tcgccggcca ggtggtcgag cggatcctgc cgaacggcct gaaggtgctg
      841 atggtgaagc gcaccgaggc accgctcatc cgctgcatcc tggcctaccg cgtgggcgcg
      901 gtgaacgagc ggccgggcat caccgggatt tcgcacttcc acgagcacat gatgttcaag
      961 ggcactcgct cgatgggcat caagcccggc accttcgccc gggacgagga gtacaaccgc
     1021 cagatcgatg cgctcatgga ccaggtggcc gcggaggaag cccggcctgg cgccagggac
     1081 gaggcgaaga tcgccgggct gaagcagcaa gcgtcggccc tgctcgagcg gcagaagaag
     1141 gagaccatcg tcagcgagga gatctggtcg gcctatcagg cggcgggcgg caccggcatc
     1201 aacgcgtcga ccgggtccga gatgactcag tactacgtga cgctgccgaa gaacaccctc
     1261 gagctgtacc tggcgctcga agcggatcgc atggcccacc cggtgttccg ggagttctac
     1321 tcggagcggg acgtggtcgc cgaggaacgc cgcatgagcg agaactcccc gggcttcttc
     1381 ttccaggagc agctcaacgc gacgttctac gcggcgcatc cctacgccaa cccggtggtc
     1441 ggctggatgt ccgacatcag ccgcgtcacc aagcaggaga tgatcgcgta ccgggagatc
     1501 tactacaggc ccgacaacgc gaccctcgtg ctcgcgggcg atctggaccc ggagcagacg
     1561 atggcccttg tcgaaaagca cttcgggggc atcgcgtctc gggggccgtc gccgcgcgtc
     1621 cgcaccgagg agccatcgcc cttctactat cgccggacca tcggcgggag cttcaagccg
     1681 ccgttcatcg agaagcgcgt gatcggccgg gctgcaacga acccgcaggt cgtgctcatg
     1741 ttccatatcc cgccgctgtg gcacgacgat ctgcccgccc tctacatgct ggggcgggtg
     1801 ctcggcgcgc gcacgggccg gatgtacctc gacctggtcc aggcgaaaga gcacgcgacg
     1861 tcggtgatgg cgtcggcgtc cgactcgatg tacgacggcg tctttcgcgt cagcgccacg
     1921 ggccgggagg tgcaagggga gctggcggcc cctctggacc agatcgagaa ggggctctgg
     1981 tcgttcatcg aggacgccaa gtccgttccg gtcgatccgc agctgctgga gcgcacgaag
     2041 aactccgtcg aggcgcagta cctccagggc ctcgccggca ccgggatcgc cggcaccctg
     2101 gcgcggatgg agacagccta ccgctggcag tacatcgacg agcaatacag gcgccggatg
     2161 gccgtcactc cggacgacct gatgcgggtg gcgaaggcgt atctcacccg cgacaactgc
     2221 gtgaccggca tcctggagcg cgagcagtag ccgcgcgcct gacagggagg gcggaccgat
     2281 gctgaagaga ctgtcggcgg tgctgctcgt cggtgcgttc gtcacggccg ccgccgcggc
     2341 cgcgcaacag acagccgtga agccgcgcaa gacgcccggg gctgcggcgg tcgcgccgga
     2401 gcgggccgaa cagggcccaa ggcccgagca cttcaccttt ccgccgctcg cgttcacgcc
     2461 gcccaaggcg gcggactacc gggcccagct ctcgaacggc ctggtggtgt acctggcgga
     2521 agaccacgcc atcccctggt tcgaggcgat gctgctgacg cccgttgcgg gcggcgccgc
     2581 gggcttcggc cggggccgcg gagcgggccc ggacgagggc gcgccggacc tgcccggccg
     2641 gctgcgcttg tcggggcgcg gcgggtcctg gccgcagggc cgtggcggcg gcgggggagg
     2701 cccgaagtcc ttcctggagc ccgccgacaa gctgggcatt caggatctga cggcatcggt
     2761 gatgcggacg ggcgggacga cctcgatgag cgcggaccag atcaacgagc gcatggaatt
     2821 cctcgccggt tccgtgtcgc cgacgtcgct ctcgattcac atgcgccacc tggatgaggg
     2881 gctgcggatc tggctcgaca tcctgaccag tcccgcgttt ccggaagacc ggctgcgccg
     2941 cgagcggcag gcgatgaccg cgccgctgcg gaaccggaac cgcaatctca cggccgtcgc
     3001 cagtgacacc tgggggaagc tgatctacgg cgaggcctcc cccgtcacgg cagaggcgac
     3061 cgaggccacg atcaacagca tcacgcgaga cgacgtcgtc gcctggcacc gcaagtactg
     3121 gggggcgaac aacgcgatac tcgtcgcggc gggcgacttc acgaaggccg agatgctccg
     3181 gcggctggag gcgaccttcg ggaagtggcg agcctcggag gcgaaggcgg ccccggcgta
     3241 cccggtcgtg ccgtcggccg ccgcggcgcc gggcgtctac atggtgcagc cgctcggcgt
     3301 cacgccgaat cagggcatca ttcgggtcgg caccctctcg atcacccagg acgatcccga
     3361 ctacgcggcc gtggatctga tgaactacac cctcggcggc ggatcgttct cgtcgcgcat
     3421 cacgcagatc gtgcgcaacg actacggcct cgcctacacg gccaattcga gcgtgggggc
     3481 ggaactgcac ta
//
LOCUS       DAOSIF010000330         3479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23618_length_3479_cov_4.290888, whole genome shotgun sequence.
ACCESSION   DAOSIF010000330 DAOSIF010000000
VERSION     DAOSIF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23618_length_3479_cov_4.290888"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="PLT35_10570"
     CDS             complement(<1..1824)
                     /locus_tag="PLT35_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29708.1"
                     /translation="MRSPSWKRLASVAAVAAVAALMAGVAAGCSRSAQSYFERGNARL
                     EKGDTAAAVLEYRNAVQKDPKFAPARQKLADLYLQQGDAAGALAEAVRAADLLPDDAA
                     AQVKAGSLLLLARRAEDAKARADKALAADPKNVDAMVLRANALAGLTDLDGALKELQE
                     AALRIEPRSSFQSNLGALQMAKGNVAEAEASFRQAVATDPKSVPAQVALGQFLWSAGK
                     REEAEGAFKAALALEPANLLANRALATFCIASNRAPEAEPYFKKIAEVSGTADAKLSL
                     ADYYVRTRRPADAVSVLEELGKDARYWALARAGIADIYHSEGKNAEAYKAVDEVIAKH
                     PSLAAARVVRGRLLLADGRLDEALADGQEAVKADPQNVAAHYLLGTVYEARRDLENAA
                     RSFGEVLRLNPRATAAQTRLAMVQMQRNELPAASQLAEQAAAAQPGNVVAQLVLARSL
                     VARGELARAAEATQDLLKRFPQVPAVQTQAGMLALAKGDRAAARAAFEKALSLDDTGV
                     EALQALVGLDLQEKKAPAARARLEQRLAKTPDASPVLALAGRTWAATGDPAKGEAYLR
                     RAIEIDASNLEAYSALGSLYLSQQKPDQAIAEFGKLAALQPG"
     gene            2409..2951
                     /locus_tag="PLT35_10575"
     CDS             2409..2951
                     /locus_tag="PLT35_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29709.1"
                     /translation="MRKTLVPLACAIALGVSASMAAADPIQLVIGDSYFLGTVIPGTG
                     NPSAEQSWVNTLLSLAPDFDPDPDFTFDPGGPHQELLYRSPNAFGALPPTETYSKNDS
                     SYQDPVSGINAQSYDYLLGKYGGAAYVWVVRDLDSVWLPRKGLSHYTLFAVRQVPDGG
                     ATLVLLSAALAGLGAIRIRR"
     gene            complement(3083..>3479)
                     /locus_tag="PLT35_10580"
     CDS             complement(3083..>3479)
                     /locus_tag="PLT35_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29710.1"
                     /translation="DPGGALARRVEVLGGPAAGSLADAPGRPDRGALVRLDGLLPGSA
                     AFATAAGPASWRGSLFLAAPDQGCLVRVSGLGQSPPAPAAVERLFCGLFGRITAVLAA
                     DDGLYFAAVGGGHDEAGRPTGAVYRVRGR"
BASE COUNT          465 a         1261 c         1259 g          494 t
ORIGIN      
        1 cccgggctgg agggcggcga gcttgccgaa ctcggcgatg gcctggtcgg gcttctgctg
       61 cgagaggtag agggagccga gcgccgagta ggcctcgagg ttggaggcgt cgatctcgat
      121 ggcgcggcgg aggtacgcct cccccttcgc agggtcgccg gtcgcggccc aggtgcgccc
      181 cgccagcgcg agcacggggc tcgcgtccgg cgttttggcg agccgctgct ccagccgggc
      241 gcgagccgca ggggccttct tctcctggag gtccaggccg acgagggcct ggagggcctc
      301 gacgccggtg tcatcgagcg acagggcctt ctcgaacgcc gcccgcgcgg cggccctgtc
      361 gcccttcgcg agggcgagca tgccggcctg ggtctggacg gccggcacct gcgggaaccg
      421 cttcagaagg tcctgggtgg cctccgcggc ccgggccaat tcgccgcgcg ccacgaggct
      481 gcgcgccagg acgagctggg ccacgacgtt ccccggctgg gccgccgcgg cctgctcggc
      541 gagctggctc gccgccggca gctcgttgcg ctgcatctgg accatcgcca ggcgcgtctg
      601 ggcggccgtc gcccgcgggt tcagccgcag cacctcgccg aaggaccggg cggcattctc
      661 gaggtcgcgc ctggcctcgt aaacggtgcc gagcaggtag tgggccgcga cgttctgggg
      721 gtcggccttg acggcctcct ggccgtcggc aagcgcctcg tccagccgcc cgtcggcgag
      781 caggagccgg ccgcggacga cgcgggccgc ggcaagggag gggtgcttcg cgatgacctc
      841 gtctaccgcc ttgtaggcct cggcgttctt gccctccgag tggtagatgt cggcgatgcc
      901 ggccctggcc agcgcccagt agcgggcgtc cttgcccagc tcctcgagca ccgagacggc
      961 gtcggccggg cgccgcgtcc tcacgtagta gtcggccagg gagagcttcg catccgccgt
     1021 ccccgagacc tcggcgatct tcttgaagta cggctcggct tcgggggcgc ggttggaggc
     1081 gatgcagaag gtggcgagcg cgcggttcgc gaggaggttg gcgggctcga gggcgagggc
     1141 ggccttgaag gcgccctcgg cctcctcgcg cttgcccgcg ctccagagga actgcccgag
     1201 cgccacctgg gccgggaccg acttcgggtc ggtcgcaacg gcctggcgga acgacgcctc
     1261 ggcctccgcc acgttgccct tggccatctg gagcgcgccg aggttgctct ggaaactcga
     1321 gcgcggctcg atgcgcagcg ccgcctcctg cagctccttc agcgcgccgt cgaggtcggt
     1381 gaggccggcc agggcgttgg cgcgaagcac catcgcgtcc acgttcttcg ggtcggccgc
     1441 gagggccttg tccgcgcggg ccttcgcgtc ctcggcccgc ctggccagga gcagcagcga
     1501 gccggccttc acctgcgccg cggcgtcgtc tggcagcagg tcggccgcgc gaacggcctc
     1561 ggcaagcgcg ccggcggcgt cgccctgctg caggtacagg tcggcgagct tctggcgcgc
     1621 cggggcgaac ttgggatcct tctgcacggc gttgcggtac tccagcaccg ccgccgcggt
     1681 gtcgcccttc tcgagccgcg cgttgccgcg ctcgaaatag ctctgggcgc ttctcgagca
     1741 gccggccgcg acccccgcca tgagcgccgc tacggccgcc acggccgcta ccgaggccag
     1801 ccgcttccag gatgggcttc gcatagatcg tgcgcctcca gcgcgaagat caagacgggg
     1861 ccggccggaa ctaccgccgg cgaaccctcc accacacgaa cagggccaac aagccaaggg
     1921 caaggagcag cagcgacgac ccggcctcgg gcacgcaggc atgcatagcg ttggacgact
     1981 ccaccgcgaa gaatagcacg ccgcaggcgc gccgcgcatg ggatagcagc cggaaatcgg
     2041 ggtctcggtg cccggagccg ggcgcccgga gtcggttccc cgccccttcg cctggcctcg
     2101 tggcagcccg ccctcctctt accccctcgc atgtttcggc gcttctcggg cgggggtgca
     2161 ggttttccac gctctggcag actaacgtcg gcggcattga caccagggct ggactgcgcc
     2221 ctttggggcc agggattccg gcgaagcggc aactcaaacg ctggcggtac ttcctgtcct
     2281 tcagcaaatt acgagtggcc gcacgcggcg aaaccgtggc ctgtcgtcat tctggacact
     2341 ggtgcgattc ttgctgtaac attgggtgaa gccgaacccg aaccggcgtt gggccgcgag
     2401 gagcttcgat gagaaagaca ctcgttcccc ttgcgtgcgc gatcgcgctc ggcgtgtcgg
     2461 cctcgatggc cgcggctgac ccgatccagc tggtcatcgg cgacagctac ttcctgggaa
     2521 cggtcatccc gggaaccggc aacccgtcgg ccgaacagtc gtgggtgaac acgctcctgt
     2581 cgctcgcgcc ggacttcgac ccggatcccg acttcacctt cgatccgggg gggccgcacc
     2641 aggagctcct gtatcgatcg cccaacgcct tcggcgcgct gccgccgact gagacatact
     2701 ccaagaacga cagctcgtac caggatccgg taagcggcat caacgcgcag tcgtacgact
     2761 acctcctcgg caagtacggc ggcgcggcgt acgtctgggt cgtgcgcgac ctggactcgg
     2821 tctggctgcc ccggaagggg ctgtcccact acacgctgtt cgccgtccgc caggtccccg
     2881 acggcggggc gacgctcgtc ctgctcagcg cggccctcgc ggggctgggc gccatcagaa
     2941 tccgccgcta gccccggcca gcgcggagga accagggccg gagccgcttc cggcccttcg
     3001 cacgtacgcc tgcgttgaag agccggcgtc gctgggacgg ccctcggcgc aggccggatc
     3061 gggcccgggg cgacgggcgc cgctaccggc cgcgcacgcg gtagaccgcg ccggttggcc
     3121 gcccggcctc atcgtgcccg ccgccgacgg ccgcaaagta gagcccgtcg tctgccgcca
     3181 gcacggcggt gatgcggccg aagaggccgc agaacaggcg ctcgacggct gccggtgccg
     3241 gcggcgactg gccgaggccc gacacgcgaa cgaggcagcc ctggtccggc gccgcgagga
     3301 agaggctgcc gcgccaggag gccgggccgg ccgccgtggc gaaggcggcc gaacctggca
     3361 gcaggccgtc cagccgcacg agggcgccgc ggtccggccg ccccggggcg tcggccagtg
     3421 atccggccgc cggccctccc aggacctcca cgcgccgcgc cagcgcgccg cccgggtcc
//
LOCUS       DAOSIF010000331         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23637_length_3477_cov_3.612507, whole genome shotgun sequence.
ACCESSION   DAOSIF010000331 DAOSIF010000000
VERSION     DAOSIF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23637_length_3477_cov_3.612507"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1176
                     /locus_tag="PLT35_10585"
     CDS             400..1176
                     /locus_tag="PLT35_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29711.1"
                     /translation="MQIRYYMMCSRFEALIASHLEPEAFGTYMAVGTKKLASGHVTFF
                     EIDPGFARLTDHFQVADIEQRCVPHEDGSPKRSKYISVYRVMEFLPLSVYGSLYLTTP
                     DGRVLKLDGEAYENNDEQDGVSLYQELCPLNPLVVSKLAPARFARVLTDPTNPVHAPR
                     LFFVDLQADRDEKGRLAGWLPYSNPMHIEDCIQELERSGPEMKATKTVSRTPLAQNFL
                     RVIRRGFFLGDQSGVKCYRFPTRDELEIRHSQWWHSASAQ"
     gene            1246..>3477
                     /locus_tag="PLT35_10590"
     CDS             1246..>3477
                     /locus_tag="PLT35_10590"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOG29712.1"
                     /translation="MVPRLFRVKSWWPVVLLLGFGAALFWRPAGAALAAAAQDAGHAP
                     GAFRFFSPFTDPRFSAVEQVALLVVLAIAVAALLYAAALYRQVNRAGSGSPKMQEVAS
                     AIREGANSYLAAQFRRLAPLIALITILLALSYTGHESAFRWGRAGAFLVGALFSWTVG
                     FVGMRLATTGNLRVAAAAMSSYGEAMQLGYRTGTVTGMLTDGLGLLGGTAIFLLYGEQ
                     AYEALLGFGFGGTLLALFMRVGGGIYTKAADVGADLVGKIEKDIPEDDPRNAATIADN
                     VGDNVGDCAGMAADIFESYEVTIVAAMILGMASFGHKGVIFPLLVRGIGVVGSIISTY
                     TVRAGADDTSDTALKTVHRGFWIGSVISTVGFFVVALGYLRFDAAYLADNPMAAAGFP
                     AGGPAALPFWANLGSAGLDLRPALTCLIGVFLAVALNKVTSYYTHTGHAPVQALAKSC
                     TTGHATNIIDGIALGYESTVAAIVVIGGAILLSVLCYAGTPPMFISYGVAMTGIGMLT
                     LTGNTISMDVFGPVADNANGIGEMGYDPEAMEARRKGSYRRARQILADLDAVGNTTKA
                     ETKGIAIGSAVIAAVSLFSSFIAVIAVGSEDRIGSMTLAQYVAEAGRITVAEPTVFIG
                     LLIGGAVPFLFSSMLLRAVGRAAFFIVKECRVQFRDMEIWAGTKKPDYGRVVDICTST
                     AQKELIGPGFLAIGTPIMVGFLLGPYALGGFLAGTILSGQLLAVFMANAGGAWDNAKK
                     LIED"
BASE COUNT          511 a         1189 c         1236 g          541 t
ORIGIN      
        1 cacggacccc atcatctcgc ttcgtctgtt ccacgccctg aacggcctgg gcaagacagt
       61 ggcgctctac atgtacccgc tggaggacca cgggcccgcg accagggaga cgctgctgga
      121 tctgtgggcg cgctggaacg cgtggctgga caagtacgtc aagaacccga agccgtgacg
      181 cctcccggcc ggccccgggc ggacgacatc cgccgatggg cagcgcaacg cccgcctggg
      241 ggagggccgc cgccggcgcg gacgccctcg cggcggcggg gcacgggcgg cgcgccgagc
      301 gggtgtgata atgtcgagtg gttagaccgc cccttggtcg aggagcgggg tccagccccc
      361 ggagccgctc cgaatccaga caaccccggg aggtgcggtg tgcagatcag gtactacatg
      421 atgtgctcgc ggttcgaggc gctcatcgcc tcgcacctgg aacccgaggc cttcggcacc
      481 tacatggccg tcggcaccaa gaagctggcg agcgggcatg tcaccttctt cgagatcgac
      541 cccggtttcg ccaggctgac ggatcacttc caggtggcgg acatcgagca gcggtgcgtg
      601 ccgcacgagg acggctcgcc caaacggagc aagtacatct cggtctaccg ggtgatggag
      661 ttcctgccgc tctcggtgta cggctcgctg tacctgacca cgcccgacgg ccgcgtgctg
      721 aagctggacg gcgaggcgta cgagaacaac gacgagcagg acggcgtcag cctgtaccag
      781 gagttgtgcc cgctcaatcc gctggtcgtg tcgaagcttg cgccggcccg gttcgcgagg
      841 gtgctgacgg acccgaccaa tcccgtgcac gcgccgcggc tcttcttcgt ggacctgcag
      901 gcggatcgcg acgagaaggg ccggctggcc ggctggctgc cctactcgaa cccgatgcac
      961 atcgaggact gcatccagga gctcgagcgc agcggccccg agatgaaggc gaccaagacc
     1021 gtgtctcgca cgccgctggc gcagaacttc ctgcgggtca tccgccgggg gttcttcctc
     1081 ggcgaccagt cgggcgtgaa gtgctaccgg ttcccgacgc gcgacgagct ggagatccgc
     1141 cactcgcagt ggtggcactc ggcctcggcg cagtagcgcg cggcggcccg ggccatcggc
     1201 atcgaacgtg tgagtccccc gaaaggcagt tgacttgtga gtcgcatggt gccccgtctg
     1261 tttcgtgtca agagctggtg gccggtcgtg ctcctgctgg ggttcggcgc ggccctcttc
     1321 tggcggcccg ccggcgcggc gctggcggcc gccgcccagg acgccggtca cgcgcccggg
     1381 gcgttccgct tcttctcgcc gttcacggac ccgcggttca gcgcggtgga gcaggtcgcg
     1441 ctgctcgtcg tgctggccat cgcggtggcg gcgctgctct acgccgccgc gctctaccgg
     1501 caggtgaacc gcgccgggag cggctcgccg aagatgcagg aggtggcgtc cgccatccgc
     1561 gagggcgcca actcgtacct cgcggcgcag ttccgccgcc tggcgccgct catcgcgctc
     1621 atcacgatcc tgctggcctt gagctacacc ggccacgagt cggcgttccg gtggggccgg
     1681 gcaggggcgt tcctggtcgg cgcgctgttc agctggacgg tcgggttcgt cggcatgagg
     1741 ctggcgacga cgggcaacct gcgggtggcg gcggcggcga tgagcagcta cggcgaggcc
     1801 atgcagctcg ggtaccgcac ggggacggtg acgggcatgc tgaccgacgg cctcgggctg
     1861 ctcggcggca cggccatctt cctgctctac ggcgagcagg cctacgaggc cctgctcggg
     1921 ttcggcttcg gcggcacgct gctggcgctc ttcatgcgcg tcggcggcgg catctacacc
     1981 aaggcggccg acgtcggcgc cgatctggtc gggaagatcg agaaggacat ccccgaggac
     2041 gatccgcgca acgccgccac catcgcggac aacgtcggcg acaacgtggg cgactgcgcg
     2101 ggcatggcgg ccgacatctt cgagagctac gaggtgacga tcgtcgcggc gatgatcctc
     2161 gggatggcga gcttcggcca caagggcgtc atctttccgc tgctcgtccg cggcatcggc
     2221 gtggtcgggt cgatcatcag cacctacacc gtcagggcgg gtgcggacga cacgtcggac
     2281 acggcgctga agacggtgca ccgcggcttc tggatcgggt cggtgatcag caccgtcggg
     2341 ttcttcgtgg tggcgctggg ctacctgcgg ttcgacgcgg cgtacctcgc cgacaacccc
     2401 atggcggcgg ccggcttccc ggccggcggc ccggcggcgc tgccgttctg ggcgaacctc
     2461 gggagcgcgg gcctcgacct ccggccggcg ctgacgtgcc tcatcggcgt gttcctggcc
     2521 gtggccctca acaaggtgac gagctactac acgcacaccg gccacgcgcc ggtgcaggcg
     2581 ctggcgaagt cctgcacgac cggccacgcg acgaacatca tcgacggcat cgccctcggc
     2641 tacgagagca ccgtcgcggc catcgtcgtc atcggcggcg ccatcctgct gtcggtgctc
     2701 tgctacgcgg gcacgccgcc gatgttcatc tcgtacggcg tcgccatgac cggcatcggg
     2761 atgctgacgc tgacgggcaa cacgatctcc atggacgtgt tcgggccggt ggccgacaac
     2821 gccaacggca tcggcgaaat ggggtacgac cccgaggcga tggaggcccg gcgcaagggc
     2881 agctacaggc gcgcccggca gatcctggcc gacctcgacg ccgtgggcaa caccaccaag
     2941 gccgagacga agggcatcgc gatcgggtcc gccgtcatcg cggccgtgtc gctgttcagc
     3001 agcttcatcg cggtcatcgc cgtcggcagc gaggaccgca tcggcagcat gacgctggcc
     3061 cagtacgtcg ccgaggcggg gcggatcacg gtggccgagc ccaccgtgtt catcgggctg
     3121 ctcatcggcg gcgcggtgcc gttcctcttc tcgagcatgc tgctccgcgc ggtcggccgc
     3181 gcggccttct tcatcgtcaa ggagtgccgc gtgcagttcc gcgacatgga gatctgggcg
     3241 ggcacgaaga agcccgacta cggccgcgtc gtggacatct gcacctcgac ggcgcagaag
     3301 gagctcatcg ggccggggtt cctggcgatc ggcacgccca tcatggtggg cttcctcctc
     3361 ggcccgtacg ccctcggcgg cttcctcgcc ggcacgatcc tgagcggcca gctgctcgcg
     3421 gtcttcatgg ccaacgccgg cggcgcctgg gacaacgcga agaagctcat cgaggat
//
LOCUS       DAOSIF010000332         3476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23645_length_3476_cov_3.463023, whole genome shotgun sequence.
ACCESSION   DAOSIF010000332 DAOSIF010000000
VERSION     DAOSIF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23645_length_3476_cov_3.463023"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2379
                     /locus_tag="PLT35_10595"
     CDS             <1..2379
                     /locus_tag="PLT35_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29713.1"
                     /translation="AVISEQRLEQVDQDRTESFYAASGALEKLSADLGNLFTTTYAPT
                     VAQVTALNASPPSSAQLPGVTYVTTSEGPGYGITSPPDANGDGFPDAVQRSITSGTFQ
                     GFTGLVTGYTAAVTARTGNVGEVRLQRQIQTVAIPLFQFGQFSEFDLGFHPAYDFDFG
                     GRIHTNGNLYLAAGTTLTIGDKITVLGEVVRKYAMNGQPTSSSWPGTVRIIKAPGSYR
                     ALAFTEGSVVESVGSALNEPTWSNLSIGTYHGYIVNGRTGARRLDLPVVAAGANPIEL
                     IRRPAPGETTSSPVFPQRYFAMASLRILLSDTAADITSLPTVTGTAPIQLGTVEPAGY
                     TVAAATPEFAQAPSVISKTNYRTNANAPLLGGYIKIEKMTSENPPTWVDVTLDILKLG
                     IASKNLSKSGCLDPSPNAVIRIQRVKDQTACNASSTAGSNFWPKVLYDTREGLRRENE
                     STSSTNVYLGGVMHYIELDVYNLSRWFMGLIGSSGSGAVRTTGYTVYFSDRRGNRNSS
                     NVETGEYGAEDFVNPASSTGAANGALDAGEDVNDNKVLDVYGQTPRGPSEVTSWSSPL
                     DSTARPWTAVTPGVAQVNPPRFFRRALKLVNGTEGNISLGSSGGVRLGLTVASENPVY
                     VEGNYNATSSGFGASHVACSVAADAVTMLSSNWNDNSSFSTPNKASSRPATTTRYRLA
                     VLTGKGRPFPRPSAGSPPVNFGTDGGTHNFLRMLEDWSGATFYYRGSMASLYYNRQAL
                     GTFKDGDNTYSPPTRDFLFDTEFLTPALLPPRTPMFRDVNSLGFTQVLTPPQ"
     gene            2812..>3476
                     /locus_tag="PLT35_10600"
     CDS             2812..>3476
                     /locus_tag="PLT35_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOG29714.1"
                     /translation="MLSSVRAFVLAVFVAMAAAAPPQTASAPSPSDLARAIQRKYDTV
                     RDLSADFVHAYRGGVLRREATERGTMRIKKPGRMRWVYEDPERKEFVSDGLRIYSYIP
                     EDKQVLVAPVPSAEEATTPALFLSGHGNLARDFIASYPDSPDSAPGTWTLTLTPRRPE
                     AEYESLTLVVDRGTCRLRKLITADAQGGRSVFTFTDVRENVGLPDSLFRFAIPRGVEV
                     VAHG"
BASE COUNT          555 a         1262 c         1164 g          495 t
ORIGIN      
        1 gcggtgatca gcgaacagcg cctggagcag gtggatcagg accggaccga gtcgttctac
       61 gcggcaagcg gcgccctcga gaagctgtcg gccgacctgg gcaacctctt cacgacgacg
      121 tacgcgccca ccgtggcgca ggtgacggcg ctcaacgcga gcccgccgtc gagcgcccag
      181 ctgccaggcg tcacctacgt cacgacgagc gagggcccgg ggtacggcat cacgtcgccc
      241 cccgacgcga acggggacgg gttcccggac gccgtccagc gatcgatcac ctcggggacc
      301 ttccagggct tcacggggct ggtcaccggg tacacggccg cggtcaccgc gcggacgggc
      361 aacgtcggcg aggtcaggct gcagcggcag atccagacgg tcgccatccc cctcttccag
      421 ttcgggcaat tctccgagtt cgacctcggc ttccaccccg cgtacgactt cgacttcggg
      481 gggcggatcc acacgaacgg gaacctctac ctggcggccg gcaccacgct gaccatcggg
      541 gacaagatca ccgtgctcgg cgaggtcgtc cggaagtacg cgatgaacgg gcagccgacc
      601 agttccagct ggccagggac cgtgaggatc atcaaagcgc cgggctccta tcgcgcgctc
      661 gccttcaccg agggcagcgt cgtcgaatcg gtcgggtcgg cgcttaacga gccgacctgg
      721 agcaacctgt cgattggcac ctatcacggg tacatcgtga acggccgaac cggggccagg
      781 cggctcgacc tgcctgtcgt ggcggccggg gcgaacccca tcgagctcat ccggcggccg
      841 gcgccgggcg agacgacgtc ctcgcccgtg ttcccgcagc gctacttcgc gatggcgagc
      901 ctgcgcatcc tgctgtcgga cacggccgcc gacatcacca gcctcccgac ggtgacgggc
      961 acggcgccga tccagctcgg caccgtcgag ccggcgggct acaccgtggc cgcggcgacg
     1021 ccggagttcg cgcaggcgcc gtcggtgatc tccaagacca actaccggac caacgccaac
     1081 gcgccgctgc tcggcggcta catcaagatc gagaagatga cctccgagaa cccgcccacc
     1141 tgggtggacg tcacgctcga catcctgaag ctggggatcg cgagcaagaa cctgtccaag
     1201 tcgggctgcc tcgacccgag tccgaacgcg gtcatccgga tccagcgcgt gaaggaccag
     1261 acggcctgta acgcctcctc gacggccggc tccaacttct ggccgaaggt cctgtacgac
     1321 acgcgcgagg gcctgcgccg ggagaacgag tcgacatcga gcaccaacgt gtatctcggc
     1381 ggcgtgatgc actacatcga gctggacgtg tacaacctgt cgcgttggtt catgggcctg
     1441 atcggatcga gcggatccgg ggccgtgcgc acgaccggct acaccgtgta cttctcggac
     1501 cggcgcggca accgcaattc ctcgaacgtc gagaccggcg agtacggcgc ggaggacttc
     1561 gtgaaccccg cgagctccac cggagcggcc aacggcgcgc tggacgcggg cgaggacgtg
     1621 aacgacaaca aggtgctcga cgtgtacggc cagacgcccc gcgggccgag cgaggtgaca
     1681 agctggtcgt caccgctcga ctccacggcg cggccgtgga cggcggtgac gccgggcgtg
     1741 gcccaggtca acccgccccg gttcttccgg cgcgcgctca agctggtgaa cgggacggag
     1801 gggaacatca gcctgggctc ctcgggcggc gtccgcctcg gcctgacggt ggcgtccgag
     1861 aaccccgtct atgtcgaggg caactacaat gcgacgtcga gcgggttcgg cgcctcgcac
     1921 gtcgcctgct cggtcgccgc cgacgccgtc acgatgctgt cgtcgaactg gaacgacaac
     1981 agctccttca gcacccccaa caaggcctcc agccgtccgg ccacgaccac tcggtaccgt
     2041 ctggccgtgc tgaccgggaa gggccggccg ttcccgcggc cgtcggccgg gtcgccgccg
     2101 gtgaacttcg gcaccgacgg gggcacgcac aacttcctgc gaatgctgga ggactggagc
     2161 ggcgcgacgt tctactaccg cggctcgatg gccagcctgt actacaaccg ccaggccctg
     2221 gggaccttca aggacggcga caacacctac agccccccga cgcgcgactt cctgttcgac
     2281 accgagttcc tgactcccgc gctgctgccg ccgcgcaccc cgatgttccg cgacgtcaac
     2341 tcgctcggct tcacccaggt actgacgccg ccgcagtagc gcggacgaag ccgggctggc
     2401 ccaggcttcc tcgctcgcgc ctcctcgccg cgccgggcgg ggaggcgccc ttctccaccc
     2461 ccctgtacgc tgacggccgg atgctccggc ggcgggccgc accaggaaga ccgtcccggc
     2521 caggccgcca gccttcggat cggccgcgct cgacgacagg ctcccgatcc tccccatgac
     2581 cgccgcgcag atcggcgtgc cggccggagc ttcgagactg cggggcgcga accgcgttcc
     2641 gaagagagag gcccgaggca ggtcggcctc gcgcggattc catccgctgg ccgccggggc
     2701 ggcacagcac gccttgtctg ccgccagccg aacccgcgcg gccaggcggc ctgcctcttc
     2761 gccgcgggct gctcgcccgt ccgggcctgt gctatcgttg acggcgaagg gttgctgagc
     2821 tcagtgcgcg ccttcgtcct cgccgtcttc gtcgcgatgg cggccgccgc gccgccccag
     2881 accgcgtcgg cgccgtctcc gtcggatctg gcgcgggcga tccagcgcaa gtacgacacc
     2941 gtccgggatc tctcggcgga cttcgtgcac gcgtaccgcg gcggcgtgct gcggcgggag
     3001 gcgaccgagc gcggcacgat gaggatcaag aaacctggca ggatgcgctg ggtctacgag
     3061 gacccggagc ggaaggagtt cgtgtccgac gggttgcgga tctactcgta cattccggag
     3121 gacaagcagg tcctcgtcgc accggtgccc tccgctgaag aggcgaccac gcccgcgctg
     3181 ttcctgtcgg gccacggcaa cctggccagg gacttcatcg cttcctaccc tgactccccc
     3241 gattcggcgc cgggcacgtg gacgctcaca ctcacgccac gccggcccga ggccgagtac
     3301 gagtcgctca cgctcgtcgt agaccgcggc acctgccggc tgcggaagct catcacggcc
     3361 gacgcgcagg gcggccgctc ggtgtttacg ttcacggacg tccgcgagaa cgtcggcctg
     3421 ccggattcgc tcttccggtt cgccatcccg cgaggcgtcg aagtggtcgc ccatgg
//
LOCUS       DAOSIF010000333         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23734_length_3468_cov_3.168473, whole genome shotgun sequence.
ACCESSION   DAOSIF010000333 DAOSIF010000000
VERSION     DAOSIF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23734_length_3468_cov_3.168473"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLT35_10605"
     CDS             <1..748
                     /locus_tag="PLT35_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HOG29715.1"
                     /translation="DAALLTKLFEQEVPEIYDNTVVIKGAVREAGDRAKVAVYSRERD
                     VDPVGACVGMKGTRVQAIIRELRGEKIDIVEWSEDPVLFVTNALSPAKVQRVTIVDEQ
                     EKVMEVVVEDHQLSLAIGKKGQNVRLAAKLTGWRIDIKSEEEKRKEVEAQFEGLEVPG
                     GAEDGSARAAELSLPDIHDEVVAVLRAAGYAAPSQVIAAGPLALVECGLDEETVQAVL
                     ESARSVVEAPVEAGPAPADDGGETEPQSQA"
     gene            758..>3468
                     /gene="infB"
                     /locus_tag="PLT35_10610"
     CDS             758..>3468
                     /gene="infB"
                     /locus_tag="PLT35_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG29716.1"
                     /translation="MATVRIYKIAELLDTSSQEIVALLKRDHGIDVKSASSTVEEMVA
                     RQFVERLAKKRGIALPSGDMFAEGAAARIKKAAASRKAEPPPPVKPALPPPRLVKTVK
                     PPPPSAEEAAPEPAAEGPAPAVAAPPAAGAEPAVVEIMEAAPAEVVAPAAPAAPPDAA
                     PPAADVQAPPVAAAPEPEPAAAPGPALAEEAAAPAPTAPEEPAPAAAEEPAAAPTTGL
                     PPRTGRVVPPTLRLRVEEPRTSAAPPVEPPAARTIVLPKPAPPLRQKAPSASPGAGAD
                     AGAKAPVGTRPAAPPAARPMPPGLSRPPVTGGPRPLPSQPVRSQPAVGYRPGAPMAPP
                     RPAVGRPAPPPRPMGRPGARAPRRGVTRDTAPGPSVAEAPPPITRTITLAEGMTVKEL
                     AEKLDVRAKDVLKRLLDRRIMMTINTTLDTETATSLAREFGADVRLRSFEEELVEVEA
                     GEAKPEDLVSRAPVVTVMGHVDHGKTTLLDAIRTTKVAEREAGGITQHIGAYQVTVND
                     RPVVFLDTPGHEAFTLMRARGARVTDIVVLVVAADDGVMPQTREAIDHARAAGVPIIV
                     AINKIDKPEANADRVMKDLSDLGLMPEAWGGQTVTVPVSAKKKQNLDLLLEMILLVTD
                     IGELKANPKAPGHGTVLEAKIDRGRGPVATVLVQNGTLQVGDTVIAGSVVGKVRALID
                     DRGRGVKSAPPSTPVEVLGLTGLPAPGDTFQVIADQAKARQVATYRQSQARDLALGTR
                     GPRVTLEGLKDKIAEGSIKELPILVKADVQGSAEVLAATLVKLSDEKVKVRIVHAGVG
                     AINLSDVLLASASGAVIIGFNVRPDRSAAELAEREGVDVRQHSVIYHVTDEIKKAMAG
                     LLEPTFREVRVGSAEVRETFRVPRFGTIAGCIVSEGRVARTADMQVRL"
BASE COUNT          547 a         1265 c         1257 g          399 t
ORIGIN      
        1 cgacgccgcg ctcctcacca agctcttcga gcaggaagtg ccggagatct acgacaacac
       61 cgtcgtgatc aagggcgccg tgcgcgaggc gggcgaccgt gcgaaggtcg cggtgtactc
      121 ccgcgagcgc gacgtggacc cggtcggcgc ctgcgtcggc atgaagggaa cgcgcgtcca
      181 ggccatcatc cgcgagctcc gcggcgagaa gatcgacatc gtcgaatggt ccgaggaccc
      241 ggtgctgttt gtgacgaacg ccctgagccc ggcgaaagtc cagcgcgtca ccatcgtgga
      301 cgagcaggag aaggtgatgg aggtcgtcgt cgaggaccac cagctctcgc tcgccatcgg
      361 caagaagggc cagaacgtcc gcctggcggc gaagctgacc ggctggcgca tcgacatcaa
      421 gagcgaggaa gagaagcgga aggaagtcga ggcgcagttc gagggactcg aggtcccggg
      481 cggcgcggag gacgggtccg cacgcgccgc ggagctgagc ctgcccgaca tccacgacga
      541 ggtcgtggcg gtgctgcgcg ccgcggggta cgccgccccg agccaggtga ttgccgccgg
      601 cccgctcgcg ctcgtcgagt gcgggctgga cgaggagacg gtgcaggccg tgctcgaatc
      661 ggccaggagt gtcgtcgagg cccccgtcga ggcggggccg gcgcccgcgg acgacggggg
      721 cgagacggaa ccacaatcgc aagcgtagag gtcagcgttg gccactgtcc ggatctacaa
      781 gatcgcagag ctcctcgaca cgtcgagcca ggagatcgtc gccctgctga agcgcgacca
      841 cgggatcgac gtcaagagcg cctccagcac cgtcgaggag atggtcgcgc gccaattcgt
      901 cgagcgcctg gccaagaagc gggggatcgc gctcccttcg ggcgacatgt tcgccgaagg
      961 ggccgcggcg agaatcaaga aagccgccgc cagcaggaag gccgagccgc ccccgccggt
     1021 caagccggcc ctgccgccgc cgcgcctcgt caagaccgtc aagccgccgc ctccgtccgc
     1081 cgaggaggcc gcgcccgaac ctgcggccga ggggcctgcc ccggccgtcg cggccccgcc
     1141 tgccgccggg gccgagccgg ccgttgtcga aatcatggaa gccgcgcccg ccgaggtggt
     1201 cgcgccagcg gcgccggcgg cgccgcctga cgccgctccc ccggccgcgg acgtccaggc
     1261 gccgccagtc gcggctgccc cggagccgga gccggccgcc gccccggggc cagccctggc
     1321 ggaagaggcg gccgcgcccg ccccgacggc gccggaagag ccggcgccgg ccgcggccga
     1381 ggagccggcc gccgcgccga cgaccggctt gccgccccgc acgggccgcg tcgttccgcc
     1441 gacccttcgg ctgcgggtcg aggagccccg cacgagcgcg gccccgccgg tcgagccgcc
     1501 tgccgcgagg acgattgtgc tgccgaagcc ggcgccgccc ctccggcaga aggccccgtc
     1561 cgcctcgccg ggcgcgggag cggacgcagg cgccaaggcg ccagtcggca ctcggccggc
     1621 tgctcctcct gcggcgcggc cgatgccgcc cggactctcc aggcctccgg ttaccggcgg
     1681 tccgcgcccg ctgccctccc agcccgtcag gtcgcagccg gccgtcgggt accgtcctgg
     1741 cgcccccatg gcgccgccga gaccggccgt cggtcggccg gctccgccgc cgcgcccgat
     1801 gggacggccc ggcgcccgcg ccccgcggcg cggcgtcacg cgcgacacgg ctcccggccc
     1861 gtccgtggcc gaggcgccgc cgcccatcac gcgtacgatc acgctggctg aaggcatgac
     1921 cgtcaaggaa ctcgccgaga agctggacgt ccgggcgaag gacgtcctga agcgcctgct
     1981 cgaccgccgc atcatgatga cgatcaacac gacgctcgac acggagacgg ccacctcgct
     2041 cgcccgcgag ttcggggccg acgtccggct ccgcagcttc gaagaagagc tcgtggaggt
     2101 cgaagccgga gaggccaaac ccgaagacct ggtgtcccgg gcgccggttg tcaccgtcat
     2161 gggccacgtc gatcacggca agaccacgct gctcgacgcg atccgcacga cgaaggtcgc
     2221 cgagcgcgag gccggcggca tcacccagca catcggcgcc taccaggtga ccgtcaacga
     2281 ccggccggtc gtcttcctcg acactccggg gcatgaagcg ttcacgctca tgcgcgcgcg
     2341 cggcgcccgc gtcacggaca tcgtcgtcct ggtcgtggcg gccgacgacg gcgtcatgcc
     2401 gcagaccagg gaagccatcg accacgcccg cgccgccggc gtgccgatca tcgtcgccat
     2461 caacaagatc gacaagcccg aggccaacgc ggaccgggtg atgaaggacc tgtcggacct
     2521 cggcctcatg cccgaggcgt ggggcggcca gacggtcacc gtcccggtgt ccgccaagaa
     2581 gaagcagaac ctggatctgc tgctcgagat gatcctgctc gtcacggaca tcggggagct
     2641 gaaggccaac ccgaaggcgc ccgggcacgg gaccgtcctg gaggccaaga tcgacagggg
     2701 gcgcggcccg gtggcgaccg tgctggtgca gaacgggacg ctgcaggtgg gcgacaccgt
     2761 cattgccggg tccgtcgtcg gcaaggtgcg cgcgctcatc gacgaccgcg gccgcggcgt
     2821 gaagtcggcg ccgccgtcga cccctgtcga ggtgctgggc ctcaccggcc tgccggcgcc
     2881 cggcgacacc ttccaggtca tcgcggacca ggcgaaggcg cggcaggtgg ccacgtaccg
     2941 ccagagccag gcccgcgatc tcgctctcgg gacgaggggg ccgcgcgtga ccctcgaggg
     3001 cctcaaggac aagatcgccg agggcagcat caaggagctc ccgatcctcg tcaaggccga
     3061 cgtccagggc tccgccgaag tgctggccgc cacgctcgtg aagctgtcgg acgagaaggt
     3121 gaaagtccgg atcgtccacg ccggggtcgg cgccatcaac ctgtccgacg tgctgctggc
     3181 gtcggcgtcg ggcgcggtta tcatcggctt caacgtgcgc cccgatcgca gcgcggccga
     3241 actcgccgag cgcgaaggcg tggacgtccg gcagcactcg gtcatctacc acgtcaccga
     3301 cgagatcaag aaggccatgg ccgggctcct ggagccgacc ttccgcgagg tgcgcgtcgg
     3361 cagcgccgag gtgcgcgaga cgttccgggt gccgaggttc ggcacgatcg ccggctgcat
     3421 cgtcagcgag ggccgcgtgg cgcggaccgc ggacatgcag gtccggct
//
LOCUS       DAOSIF010000334         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23765_length_3465_cov_3.938416, whole genome shotgun sequence.
ACCESSION   DAOSIF010000334 DAOSIF010000000
VERSION     DAOSIF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23765_length_3465_cov_3.938416"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLT35_10615"
     CDS             <1..486
                     /locus_tag="PLT35_10615"
                     /inference="COORDINATES: protein motif:HMM:NF016089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOG29717.1"
                     /translation="KLARGPMIAITLLRVLVGWHFLYEGIAKITSPSWSAAGYLKQAR
                     GPFADIFRWLAAQPDLLAAADSITMWGLTLVGVLLILGLFTRLASLGGIGFILLFYLC
                     NPPFVGLFYSIPVEGSYLIVNKNLVELGALIVVLASGSGVFAGLDTLVHAVLGRKRKT
                     G"
     gene            complement(611..1666)
                     /gene="tsaD"
                     /locus_tag="PLT35_10620"
     CDS             complement(611..1666)
                     /gene="tsaD"
                     /locus_tag="PLT35_10620"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG29718.1"
                     /translation="MIILGIETSCDETAAAVVEDVGDAARPWVIRSSVVASQADIHRE
                     WGGVVPELASRQHIRDICGVVEGALSGAGVAPADLGAIAVTQGPGLVGSLLVGVSFAK
                     SLAATWGVPLVPVHHLAGHIESLFLHHGPLPLPAMVLVVSGGHTNLFDVRAPGAYRAV
                     GRTRDDAAGEAYDKVAKLVGLGYPGGPVIDRLAKAGNDSAIPLPVARMTHPDRNAPLQ
                     KGQWDFSFSGLKTAVLRHVKAKGAGWTPSPGEVADLCASFQRSVVEALVEKTFAAATW
                     HGATSVGIAGGVSANSRLRADAAAFGRRAGIPVYVPPLALATDNAAMIAAAGLRRYYA
                     GVRADSRLNADAALRLE"
     gene            complement(1688..2734)
                     /gene="mtnA"
                     /locus_tag="PLT35_10625"
     CDS             complement(1688..2734)
                     /gene="mtnA"
                     /locus_tag="PLT35_10625"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG29719.1"
                     /translation="MLPAIEWRDGAVVMIDQRKLPSSEVWVTCTTAREVARAITSMVI
                     RGAPAIGVAAAMGIALGARRSAATGTRQLAAEFQKACDLMAGTRPTAVNLFWAIERMK
                     RTFAAVALAGASAGEIRAALEREALAIHDEDVEHCREIGRVGASFVPDGARILTHCNA
                     GALATAGYGSALGVIRAAVEAGKRVSVVADETRPFLQGARLTAWELVRDGIDTTVITD
                     SMAAAMMQQRQVDLVIVGADRIAANGDFANKIGTYGLAVLARAHGIPFYSAAPVSTID
                     LGTPDGSGIPIEERPAREVTHVGPVRLTPAGAAIRNPAFDITPHALVAAIFTERGACE
                     PPFEQSLRRAVAGR"
     gene            complement(2811..3206)
                     /locus_tag="PLT35_10630"
     CDS             complement(2811..3206)
                     /locus_tag="PLT35_10630"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOG29720.1"
                     /translation="MADEATLIGAARAARERAVAPYSGFKVGAALETAGGAIVTGCNI
                     ENATYGLTVCAERVALLKAVSEGHRAFRRIAVVAATEDATPPCGPCRQLLWEFCGDIE
                     VVLANLERDLGHHRLAELLPLPFDARFLS"
BASE COUNT          481 a         1288 c         1178 g          518 t
ORIGIN      
        1 aagctcgccc gcggtccgat gatcgcgatc acgctgctgc gggtcctcgt cgggtggcac
       61 ttcctctacg agggcatcgc gaagatcacc tcgccctcgt ggtccgccgc gggctacctc
      121 aaacaggccc ggggaccatt cgccgacatc ttccgctggc tggcagcgca gcccgacctg
      181 ctcgccgcgg ccgactcgat cacgatgtgg gggctgacgc tggtcggcgt gctgctgatc
      241 ctcgggctct tcaccaggct cgccagcctc gggggcatcg ggttcatcct gctgttctac
      301 ttgtgcaatc cgcccttcgt gggcttgttc tactcgatcc ccgtcgaggg cagctacctc
      361 atcgtgaaca agaacctcgt cgagctgggc gccctcatcg tcgtgctggc gtcgggcagc
      421 ggcgtgttcg cggggctgga cacgctcgtg cacgccgtcc tgggacggaa gcggaagacg
      481 gggtagtccg ggattcggga ttcccttcgg ctcgcctgcg cctccgctcg ctcagggcag
      541 gcgggattcg gggaccggga gctgggggct ctggattctg actcctgacc cctgactccc
      601 gtcttctgcc ttactccaag cgcagcgcgg catcggcgtt gagccgcgaa tccgcccgca
      661 cgccggcgta gtaccgccga agccccgccg cggcgatcat ggccgcgttg tcggtggcga
      721 gcgccagcgg cggcacgtag accgggatgc cggcccgccg gccgaacgcc gccgcgtcgg
      781 cgcgcagccg gctgttggcc gaaacgccgc cggcgatgcc gacgctcgtc gcgccgtgcc
      841 acgtcgcggc cgcgaaggtc ttttcgacca gcgcctccac caccgatcgc tggaagctcg
      901 cgcacaggtc cgcgacctcg cctggcgacg gcgtccagcc cgcgcccttc gccttgacgt
      961 gacggagcac ggcggtcttg aggccgctga agctgaagtc ccattggccc ttctgcagag
     1021 gcgcgttgcg atccggatgc gtcatgcggg cgacgggcag cggaatggcg ctatcgttgc
     1081 cggccttcgc caggcggtcg atgacgggcc cgcccgggta ccccaggccg acgagtttgg
     1141 caaccttgtc gtaggcctcg ccggccgcat cgtcccgcgt gcgcccgacc gcgcggtacg
     1201 cgcctggcgc ccgcacgtcg aacaggttcg tgtgaccgcc ggacacgacg agcaccatcg
     1261 ccggaagcgg aagcggcccg tggtggagaa acagcgactc gatgtgcccg gccaggtgat
     1321 gcacgggaac gagcggcacg ccccaggtcg cggccagcga cttcgcgaac gacacgccga
     1381 caaggagcga gccgacgagg ccggggccct gcgtgacggc gatcgcgccc aggtccgccg
     1441 gcgcgacgcc ggcgccgctg agcgcccctt cgacgacgcc gcagatgtcg cggatgtgct
     1501 ggcgggaggc gagttccggc acgacgcctc cccactcgcg gtggatgtcc gcctgtgagg
     1561 ccacgacgct ggatctgatg acccacggcc gcgcggcgtc tccgacgtcc tccacgacgg
     1621 cggccgccgt ctcgtcgcac gaggtttcga ttcccagaat gatcatgctc tcacgccggc
     1681 cgccgcttca gcggccggcg accgcccggc gcagggactg ctcgaacggc ggctcgcagg
     1741 cgccgcgctc ggtgaagatc gcggcgacga gcgcgtgcgg cgtgatgtcg aacgccgggt
     1801 tgcggatcgc cgcgcccgcc ggggtcaggc ggaccggccc cacgtgggtc acttcgcgcg
     1861 cggggcgctc ctcgatggga atgccggagc cgtccggcgt gcccagatcg atcgtcgaca
     1921 ccggcgccgc cgagtagaac gggatgccgt gggccctggc gagcaccgcg aggccgtagg
     1981 tgccgatctt gttggcgaaa tcgccgttgg cggcaatgcg gtccgcgccg acgatgacga
     2041 gatcgacctg gcgttgctgc atcatggcgg ccgccatgct gtcggtgatg actgtcgtgt
     2101 cgatgccgtc gcggacgagt tcccacgccg tgagcctggc accctgcagg aacggccgcg
     2161 tctcgtcggc gacgaccgac actcgcttgc cggcctcaac cgccgcccgg atgacgccga
     2221 gggcgctgcc gtagccggcc gtcgccaggg ccccggcatt gcagtgcgtg aggatccgcg
     2281 cgccgtccgg cacgaacgag gcgccgacgc gcccgatctc gcggcagtgc tccacgtctt
     2341 cgtcgtggat cgcaagcgct tcccgctcga gcgccgcgcg gatctcgccg gccgacgcgc
     2401 cggccagcgc gaccgcggcg aaggtccgct tcatccgctc gatggcccag aacaagttga
     2461 cagcggttgg ccgggtcccg gccatgaggt cgcaggcctt ctggaactcg gccgcgagct
     2521 gccgtgtccc cgtggcggcg ctgcggcgcg cgccgagggc aatgcccatg gcggcggcga
     2581 cgccaatcgc gggcgcgccc ctgatcacca tgctcgtgat cgcccgcgcc acctccctgg
     2641 ccgtcgtaca cgtcacccag acttccgatg acggcagctt ccgctggtcg atcatcacga
     2701 cggcgccgtc gcgccactca atcgccggca gcatatgtct ccatcatacg aggtggggtc
     2761 agaccccaag aagcgtgcca ttggcacgct tcttgggatc tgaccccacc tcagctcaag
     2821 aagcgggcgt cgaacgggag cggcagcaac tccgcgagcc ggtggtgtcc caggtcgcgc
     2881 tcgagattgg ccagcaccac ctcgatgtcg ccgcagaact cccacaacag ctgccggcac
     2941 ggcccgcacg gcggcgtcgc gtcctccgtg gccgccacga cagcaatccg ccggaaggcc
     3001 cggtgcccct cggagacagc cttgagcagc gccacccgct cggcacacac ggtcaggccg
     3061 tacgtcgcgt tctcgatgtt gcagccggtg acgatcgcgc cgccggcggt ttcgagcgcc
     3121 gcgcccacct tgaacccgga gtaaggcgcc acggcccgct cgcgtgccgc acgcgccgcc
     3181 ccgatcagcg tcgcctcgtc tgccatcagc cgtcctctcc tcgtctcccc gtccacgccc
     3241 tcgtcaggcg gcgcaaacag gggccgctgc gcgacccctt gccgccgccc ttcgccggac
     3301 gttccgccac ccggccacgg cgggccaggc ccgatcgcct gcccctaggc agccgccacg
     3361 atctcttcaa tcagcgcgat gaaggccccg cgcacgcgct gccccgtcgc catgacctcg
     3421 ctgtggtcca ggggcgccgg caggacgcca gccgccatgt tcgtg
//
LOCUS       DAOSIF010000335         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23854_length_3453_cov_3.723072, whole genome shotgun sequence.
ACCESSION   DAOSIF010000335 DAOSIF010000000
VERSION     DAOSIF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23854_length_3453_cov_3.723072"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="PLT35_10635"
     CDS             complement(<1..1267)
                     /locus_tag="PLT35_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29721.1"
                     /translation="MSKRLFVLALVAGLVFGASPAQADSASQVAGGADHTCALTAGGS
                     VLCWGAGFYGQLGSGSRDGSLTPAPVVGLSSGVTAVAAGQYHTCAVTAGGGVLCWGYN
                     ADGELGDGTTDDRLTPTAVAGLDSGVESVATAAYHSCALTTGGEVLCWGANWFGQLGD
                     GTTVDSAVPVPVAGLPGPVTAIAAGGYYSCALTAGGEVLCWGANWFGQLGNGTTDASP
                     APVSVTGLAAGVATIGAGWDHACAVTAGGALLCWGSNWYGALGDGTADDRLTPTAVSG
                     LGFGVKAVTAGIYHTCALTAEGGVLCWGAVAYAGHGNPASYTLTPAAVSGLASGTASV
                     TAGAYHSCAVTVRGGVACWGSNGWGQLGDGTTADSAEPLPAVDFEGRRSTPGDFTGDG
                     IADVLWRHLTNGDMWLWGIEAAARATDAYV"
     gene            complement(1450..2385)
                     /locus_tag="PLT35_10640"
     CDS             complement(1450..2385)
                     /locus_tag="PLT35_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29722.1"
                     /translation="MRRLIAWSVPALALLALTAAGGQPPGQGPEFPPELVSFGPPSPQ
                     PLLAGTGTDTWDRRIRERGWIMREGDQWHLWYTGYNDARSDSKFLGYATSPDGLAWTR
                     YPGNPLTTTGWVEDVCVVKHGGTYYMFAEGRDDVAHMLASEDRVHWTERGSLDIRRVD
                     GQPIAPGPRGTPTVWIEGEVWWLFYERGDSAIYAATSRDLRTWTNVTDEPVIARGPDA
                     YDRFAVAVNQIVKYQGRYYAYYHASALPDWAEWSTCVAVSDDLVRWKKYPGNPVLPVN
                     PAMRGASSGTVVHDGARYRLYTTHPDVRVYYARTR"
     gene            complement(2577..>3453)
                     /locus_tag="PLT35_10645"
     CDS             complement(2577..>3453)
                     /locus_tag="PLT35_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29723.1"
                     /translation="NLIGKWDVLVFPPVGRSAQQVVAGMPMWRNPLPWKTTPLTPNIG
                     KIDSTDDMRPGLGWGGLANLEAFVRQGGLLIVADDTSVLAGTYGFTPGLSVQQPSRIN
                     APGTVMRARIVDPASPIAYGYGENLSVYSAPGLLFGVSNTMGGRRGGGPRGEGGPTRP
                     TGRGTADDHDQPQGRPFVEPEEERKVEPWQAAPVTNEQLLNPLGVIPPAQRPRVVLRY
                     GDARELLVSGLLDNGSEIAQRPLVVDVPLGKGHLVLFANNPMWRGQTQGSYALVFNAI
                     LNYDQLDAGRKLDEK"
BASE COUNT          484 a         1296 c         1166 g          507 t
ORIGIN      
        1 cgacatacgc gtccgtcgca cgcgccgcgg cttcgatgcc ccagagccac atgtccccgt
       61 tcgtcagatg gcgccagaga acgtccgcga tcccgtcgcc cgtgaagtcc ccgggcgtgg
      121 atcgccggcc ctcgaagtcg accgcgggca ggggctcggc gctgtccgcg gtcgtgccgt
      181 cccccagctg gccccagccg ttcgagcccc agcacgcaac gccgcctctc accgtgaccg
      241 cgcacgagtg ataggcgcca gccgtcaccg aggccgtgcc gctcgccagc ccgctgacgg
      301 ccgcgggcgt gagggtgtag ctcgcgggat tgccgtggcc ggcgtacgcg acggcccccc
      361 agcacaggac gccgccctct gccgtcagcg cgcacgtgtg gtagattccg gccgtgacgg
      421 ccttcacgcc gaacccgagc ccgctgaccg cggtcggcgt cagccggtcg tcggccgtgc
      481 cgtcgccgag cgctccgtac cagttggaac cccagcagag gagcgctccg ccggccgtca
      541 ctgcacaggc gtgatcccag cccgcgccga tcgtcgcgac gccggccgcc agcccggtga
      601 cgctcacggg agccgggctc gcgtccgtcg tgccgttgcc gagctggccg aaccagttgg
      661 cgccccagca gaggacctca ccgccagccg tcagcgcgca gctgtagtaa ccgcccgcgg
      721 cgattgccgt gacggggccg ggaaggcccg ccaccggcac cggcacggcg ctgtccactg
      781 tggtgccgtc gccgagctgg ccgaaccagt tggcgcccca gcagaggacc tcaccgccag
      841 tcgtcagcgc gcacgagtga taggcggctg tcgcgacgga ttcgacgccg ctgtcgaggc
      901 cggcaactgc cgtcggcgtc agccggtcgt cggtcgtgcc atcgccgagt tcgccgtcgg
      961 cgttatagcc ccagcagagc acgcccccgc cggccgtgac ggcgcaggtg tgatattggc
     1021 ccgccgcgac tgcagtgacg ccgctggaca ggccaaccac cggcgccggc gtcaggctcc
     1081 cgtctctcga gccactccca agctgcccgt agaagcccgc cccccagcac agaacggatc
     1141 cgccagccgt cagcgcgcac gtatggtcgg cgccgccggc gacttgcgac gcactatccg
     1201 cctgcgcagg tgacgcgccg aagaccaagc cggcgaccag ggccaggacg aacagccgct
     1261 tgctcatgac gactccagat gcagcgtgcc aaccggcgca ctgccgggcc tcgctcgact
     1321 cccggccgat catcggcgtg cgagaaccgc ctgtcaacac gggcccgcga caggctcggc
     1381 cgccgcctgc gcgaccgcgg caatcactcg cagcggctgc cgcacgtgac tgcgcgaggt
     1441 gcccggctcc taccgcgtcc gcgcgtagta gacgcgaacg tccgggtgcg tcgtatacag
     1501 gcggtagcgc gcgccgtcgt ggacgaccgt tccgctgctt gcgcccctca tggcggggtt
     1561 caccgggagg acggggttcc cgggatactt cttccagcgc acgaggtcgt ccgacacggc
     1621 cacgcacgtg ctccactcgg cccagtccgg caaggcgctg gcatggtagt acgcgtagta
     1681 ccgcccttgg tacttgacga tctggttgac ggccacggcg aaccggtcgt aagcgtcggg
     1741 ccctcgcgcg atgacgggtt cgtcggtcac gttcgtccag gtcctcaggt cgcgcgaggt
     1801 cgccgcgtag atggccgagt ctcccctctc gtagaacagc caccacacct cgccttcgat
     1861 ccagaccgtc ggcgtgccgc gcggccctgg cgcgatcggc tggccgtcaa cccggcggat
     1921 atcgaggctg ccgcgttccg tccagtgcac ccgatcctcg gacgccagca tgtgcgccac
     1981 gtcgtcccgg ccctcggcga acatgtagta cgttccgccg tgcttgacga cgcacacgtc
     2041 ctcgacccag ccggtcgtcg tcagcggatt gccgggatag cgcgtccagg ccagcccatc
     2101 gggagaggtg gcgtagccca ggaacttcga gtcgctccgg gcgtcgttgt agcctgtgta
     2161 ccagaggtgc cactggtcgc cctcgcgcat gatccagccg cgttcgcgaa tccgccggtc
     2221 ccaggtgtcc gtgcccgtgc ccgccagcag cggctgcggc gacggcgggc cgaacgaaac
     2281 gagttccggc gggaactccg gcccctgccc gggcggctgc cctcctgccg cggtcagggc
     2341 cagcagcgcg agcgcgggaa cgctccaagc aatgaggcgc cgcatcgcga cgcctcttct
     2401 gcgcgggcgc tcgggcgcgg gccgcggcct gcgctgacgg ccggctaggc ggcgcgcgcg
     2461 cacgacccga gtgtcgaagg cgacgcccca ggctccgttc cggcgctcga accctgcaac
     2521 gtgacgcccg gtttctgcgg ttcgatcgtc ccattccggc atctggattc ccactcctat
     2581 ttctcgtcca gcttcctccc cgcgtccagc tggtcgtagt tcaggatagc gttgaagacc
     2641 agcgcgtagc tgccctgggt ctggccgcgc cacatggggt tattggcgaa gagcacgagg
     2701 tgccccttgc cgagcggcac gtccacgacg agcgggcgct gcgcgatctc gctgccgttg
     2761 tcgaggaggc cggacacgag cagctcgcgc gcgtcgccgt agcgcagcac gacgcgcggc
     2821 cgctgggcgg gcggaatcac gccaagcggg ttgaggagct gctcgttcgt cacgggcgcg
     2881 gcctgccacg gctcgacctt ccgctcctcc tcgggctcga caaacggacg gccctgcggc
     2941 tgatcgtggt cgtcggccgt gccccggccc gtcggccgcg tggggccgcc ctcgcccctg
     3001 ggtccgcccc ccctccggcc gcccatcgtg ttgctgacgc cgaagagcag gccgggcgcc
     3061 gagtacaccg acaggttctc gccgtacccg taggcgatcg ggcttgccgg gtccacgatt
     3121 ctggcgcgca tcaccgtgcc cggggcgttg atccgcgacg gctgctgcac cgacagtccc
     3181 ggcgtgaagc cgtaagtgcc cgccagcacc gatgtgtcgt cggcgacgat cagcaggccg
     3241 ccctggcgga cgaacgcctc gaggttcgcg aggccgcccc accccaggcc cggccgcatg
     3301 tcatcggtgg aatcgatctt tccgatattg ggcgtgagcg gcgtggtctt ccagggcagc
     3361 gggttccgcc acatcggcat cccggcgaca acctgctgag cgctcctgcc gacaggcggg
     3421 aacacgagca cgtcccactt cccgatgagg ttg
//
LOCUS       DAOSIF010000336         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23987_length_3438_cov_4.437186, whole genome shotgun sequence.
ACCESSION   DAOSIF010000336 DAOSIF010000000
VERSION     DAOSIF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23987_length_3438_cov_4.437186"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLT35_10650"
     CDS             <1..406
                     /locus_tag="PLT35_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG29724.1"
                     /translation="KISMRLVPDQHPDKVAELFEAYVRKITPRTVDLKITKLHGGMPW
                     MTDFDNVYVQAAARALEQGFGKRPVLTREGGSIPIVADFQAALDAPVVLFGFGLPDDN
                     LHAPNEKFNLDNFFNGVLASAFLYREIAGTKS"
     gene            complement(526..>3438)
                     /locus_tag="PLT35_10655"
     CDS             complement(526..>3438)
                     /locus_tag="PLT35_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG29725.1"
                     /translation="PALSAQPPAKRPISYDAYDGWKSIQGVSVSRDGAWLVYTLAPQD
                     GDGELVVRNLKTGADSRYARGRGALITADSRFVVYAIAPPKADVDKAKKEKKKPEDQP
                     KSGLGVLTLSTGEVFTADRVKSFRIAEDAGGFVAYLLEAPIKKADEKKEAGAEPAPKP
                     APAADRKPKEKKKDPGTDLVIRDLASGAAATVAEVVEFAWSKDGNWLAYATSSTEATP
                     ERDGAFVRRTADGSTRTLASGPGHYKGLAFDEQSTQLAFVSDRDAYKGNPAVFKLYHW
                     TAAADAAEEAASGSTAGMPAGMVVSEHGSLEFSKDGLRLFFGYSKPPAAEPAEDAPEP
                     VKVDIWSWTDPLLQPMQKVRAEDEKKRSFRAVIHLKDKRLVPLASSGMPDLTLVDRGD
                     VALGSSDVPYLQLQSWDGDYRDYYAVSLQDGSRRKLVEKSRFPFTLSPAGTHLLGFDA
                     DDSQWYSVRVSDGARTNLTAALGVRFDDESSDTPEPPRAYGSAGWTAGDRSVWLYDKY
                     DIWEVRPDGTGARLVTGGAGRRTATVFRYARTDPDEKVIPADTPFLLSALDDVTKATG
                     FWRVTPVAPLPPSKPARTAARSARGKAAAPAPAPLPAGYGDPQKLVMLDKRLGSPGAS
                     AGPRGGGGTALLKPKDAAGPFILTAQRFDEFPDLWTADPDFRNLARVSDANPQQAQYV
                     WGRSELIEYVNADGKTLRAILTKPDDFDPAKKYPLMVYIYEILSNGLHSYATPQPGTS
                     INQARYVSNGYILLQPDIVYETGYPGEAALKCVIPAVQRVVGMGFVDPARIGIQGHSW
                     GGFQISYLITRTNMFRAVEAGASVVNMTSAYGGIRWGTGMSRAWQYERTQSRIGAPPW
                     IKTLQFLENSPLFWAEKVQTPYLTIHNDEDDAVPWYQGIEFFSALRRLGKPAWMFNFN
                     AEKHGLRERENQKYWTIHLDEFFDHYLLGKPRPSWMDDGVPYLERGKRDVSGYYRK"
BASE COUNT          495 a         1181 c         1176 g          586 t
ORIGIN      
        1 gaagatcagc atgcggctcg tgccggacca gcacccggac aaggtggcgg agctcttcga
       61 agcgtacgtg cggaagatca cgcccaggac ggtggacctg aagatcacca agctgcacgg
      121 cgggatgccg tggatgacgg acttcgacaa cgtctacgtc caggcggccg cgcgcgccct
      181 cgagcagggc ttcggcaagc ggccggtcct cacgcgcgag gggggctcga tccccatcgt
      241 cgccgacttc caggcggcgc tggacgcgcc ggttgtcctc ttcggcttcg gcctgcccga
      301 cgacaacctg cacgccccga acgagaagtt caacctcgac aacttcttca acggcgtgct
      361 ggcgtcggcg ttcctgtatc gggagatcgc gggcacgaag agctaggggc cagggaccag
      421 gcgctcggcg ctagtgggac ccggcacgcg ctgcggaaga gacacgtatc cgtcgatcag
      481 cgtgacagcg aggctgacta gcccctagcg cctagcccct agcccctact tcctgtagta
      541 cccgctcacg tccctcttcc cccgctcgag gtacggcacg ccgtcgtcca tccaggacgg
      601 gcgcggcttg ccgagcaggt agtgatcgaa gaactcgtcg aggtggatcg tccagtactt
      661 ctggttctcg cgctcgcgca gcccgtgttt ctcggcgttg aagttgaaca tccaggccgg
      721 cttgccgagc cgcctgagcg ccgagaagaa ctcgatcccc tggtaccacg gcaccgcatc
      781 gtcctcgtcg ttgtggatcg tgagatacgg cgtctgcacc ttttccgccc agaagagcgg
      841 cgagttctcg aggaactgga gcgtcttgat ccacggcggc gcgccgatgc ggctctgggt
      901 gcgctcgtac tgccacgccc gcgacatgcc cgtgccccag cggatgccgc cgtacgcgct
      961 cgtcatgttc accaccgagg ccccggcctc gaccgcgcgg aacatgttcg tccgcgtgat
     1021 gagatagctg atctggaagc ctccccagct gtgcccctgg atgccgatgc gcgcgggatc
     1081 gacgaacccc atccccacga cgcgctggac ggcggggatg acgcacttca gcgcggcctc
     1141 tcccgggtag ccggtttcgt agacgatgtc cggctggagg aggatgtagc cgttgctgac
     1201 gtagcgcgcc tgattgatgc tcgtgccggg ctgcggcgtg gcgtagctgt ggagaccgtt
     1261 gctcaggatc tcgtagatgt agaccatcag cgggtacttc ttcgccgggt cgaagtcgtc
     1321 gggcttcgtg aggatggcgc gaagcgtctt cccgtcggcg ttgacgtact cgatgagctc
     1381 cgagcggccc cagacgtact gcgcctgctg cgggttcgcg tcgctcaccc gcgccaggtt
     1441 ccggaagtcc gggtccgccg tccagagatc ggggaactcg tcgaagcgct gggccgtcag
     1501 gatgaagggg ccagcggcgt ctttcggctt gagcagcgcc gtgccgcctc cgccccgagg
     1561 cccggcgctc gcgcccgggc tgccaaggcg cttgtcgagc atgacgagct tctgcggatc
     1621 accgtagcct gccgggaggg gggccggggc aggcgccgct gccttgcccc gcgcggacct
     1681 ggccgcggtc ctcgccggct tcgacggcgg cagcggggca actggcgtga cgcgccagaa
     1741 gccagtggcc ttcgtcacat cgtccagcgc cgaaagcagg aacggcgtgt ccgccggaat
     1801 gaccttttcg tcagggtccg tgcgagcgta gcggaagacc gtggcggtcc tccggcccgc
     1861 tccgcccgtc accaggcgcg cgccggtccc gtcgggcctg acctcccaga tgtcgtactt
     1921 gtcgtacagc cagaccgagc gatcgccggc cgtccacccg gccgagccgt aggccctggg
     1981 cggctccggc gtgtcgctcg actcgtcgtc gaaccggacc ccaagcgcgg ccgtgaggtt
     2041 cgtccgcgcg ccgtcgctga cgcgcacgga gtaccactgg ctgtcgtccg cgtcgaagcc
     2101 aaggaggtgc gtgccggcgg gcgacagcgt gaaggggaag cgggacttct cgacgagctt
     2161 cctgcgcgag ccgtcctgca ggctcacggc gtagtagtcg cggtagtcgc cgtcccacga
     2221 ctggagctgc aggtacggga cgtcggacga gcccagtgcg acgtcgccgc gatcgacgag
     2281 cgtgagatcg ggcatgccgc tcgaggcgag cggcaccagg cgcttgtcct tcaggtggat
     2341 cacggcacgg aagcttcgct tcttctcgtc ctcggcgcgc accttctgca tgggctggag
     2401 cagcggatcc gtccagctcc agatgtccac cttgaccggc tcgggggcgt cctcggcggg
     2461 ctcagccgcc ggcggcttcg agtagccgaa aaagagacgc aagccgtcct tcgagaactc
     2521 gagcgagccg tgctcgctga cgaccatgcc ggccggcatg ccggccgtcg agccggacgc
     2581 ggcttcctcg gccgcgtcag ccgccgcggt ccagtgatac agcttgaaca cggccgggtt
     2641 gcccttgtac gcgtcgcgat cggacacgaa ggccagctgg gtgctctgct cgtcgaaggc
     2701 gaggcccttg tagtggccgg ggcccgatgc cagcgtcctt gtcgagccgt ccgccgtccg
     2761 ccggacgaac gcgccgtctc tctcgggcgt cgcctccgtc gaggacgtgg cgtaggcgag
     2821 ccagttgccg tccttcgacc aggcgaactc gacgacttcc gccaccgtgg ctgccgcgcc
     2881 ggaggcgaga tcgcgaatca cgaggtccgt gcccgggtcc ttcttcttct ctttcggctt
     2941 cctgtccgcc gccggcgcgg gcttcggcgc gggctccgcg ccggcctcct tcttctcgtc
     3001 ggccttcttg atcggcgcct cgagcaggta ggcgacgaac ccgcccgcgt cctcggcgat
     3061 cctgaagctc ttgacgcgat cggccgtgaa gacctctccc gtcgaaagcg tgagcacgcc
     3121 gagcccgctc ttcggctggt cctcgggctt cttcttctcc ttcttcgcct tgtcaacgtc
     3181 cgccttgggc ggcgcgatcg cgtacacgac gaaccggctg tcggcggtga tgagcgcgcc
     3241 cctgccgcgc gcgtaccgcg aatcggcgcc tgtcttcagg ttgcggacga cgagctcgcc
     3301 gtcgccgtcc tgcggggcga gggtgtagac aagccacgcg ccgtcgcggg agaccgagac
     3361 gccctggatg gacttccatc cgtcgtaggc gtcgtacgag atgggccgct tcgcaggcgg
     3421 ctgggcggac agggccgg
//
LOCUS       DAOSIF010000337         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_23989_length_3438_cov_3.435708, whole genome shotgun sequence.
ACCESSION   DAOSIF010000337 DAOSIF010000000
VERSION     DAOSIF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23989_length_3438_cov_3.435708"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="PLT35_10660"
     CDS             complement(<1..1897)
                     /locus_tag="PLT35_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOG29726.1"
                     /translation="MNTTRLALCAAFFAFAAVALTASAVAQDRLKAMPGYEQYQRTSR
                     EIPGAVQSGAPQASWRDDGRSLDFRKDGRMFRFDAATRAVSELPPDAGAPAPPPLGRR
                     GLGGAVARGRQATAAESPDKTLVAFYRDRNLFIGDASGGGEVAVTTDGSETGRIKSGT
                     ASWVYGEELAQTTAMWWSPDGRRLAYYRFDESRVPDYVLQLDQTKLYSTADVEAYPKA
                     GEPNPVVDLYVYDLAGKSSVRIDVRDGKPFTNDVVGHYVYRVAWSPDGKELLFLRANR
                     RQNVLELAAADPVTGACRVVVREEWPTGWVQTSPATVFLKDGRRFIWASERSGWQNYY
                     LYDLSGRLITPLTRYTTFEAANLVKLDEDAQVVFLMARDGDNFLKLQLHRVGLDGRTD
                     VRLTDPAFHHTVGSCLPGGGGRGGGPPAGGPAACGISADSTLAVDVYQTHDTPPALRV
                     IDAVTGRVVSELAASDTTKFAALGLRKAEMFTYLAADGKTLLRGLVSFPSNFDPSAKY
                     PALAAVYGGPASAAARETFVPPNALCEFGFLVLTLDTRAVPGMGKRALDAIYLKLGQV
                     EIDDMAEGVKALWNRPYFDRDRVGIFGTSYGGYASAMALLRHPDVFRAASASSAVTSW
                     LHYDSIYT"
     gene            2137..>3438
                     /locus_tag="PLT35_10665"
     CDS             2137..>3438
                     /locus_tag="PLT35_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOG29727.1"
                     /translation="MTQLDWIVIVFYFCLIGWVAWYYGRHQKDTTDYFLAGRNAGWVV
                     IGASIFTSNIGSEHIVGLAGQGATTGMGMAHWELHAWVLIMLAGLFVPFYYKSGVETI
                     PQFLEKRFNARARWILSCVSLVAYVFTKVSVTVYAGAVVFKALLPEAFGSPENAFWVG
                     AFTTVILTGIYTVFGGMRAIMNTATPQAVIILLGSFIVTAVGLGRLGGWGELVTMARV
                     NREAFALWRPLSDPTFPWLAVVIASPIVGIWYWCTDQYIVQRALSARDLATARRGALF
                     GGVLKVWPVLIFVIPGLIGWALHQKGLLLLPTTLVDGVAVINPDLVFATLVRELLPSG
                     LRGLIIACLLAALMSSLASLFNSSASLFTVDVYEKLRPGRSERHLLRVGRIATATVVV
                     LGILWIPVMQKVQGGGLYQYLQNVQGYIAPPITAVFLLGLFW"
BASE COUNT          476 a         1192 c         1212 g          558 t
ORIGIN      
        1 cggtgtagat gctgtcgtag tggagccacg aggtcaccgc cgacgaggcc gacgccgccc
       61 ggaacacgtc cgggtggcgc aggagggcca tcgccgacgc atacccgccg taggaggtgc
      121 cgaaaatgcc cacccggtca cggtcgaagt acggccggtt ccagagcgcc ttgacgccct
      181 cggccatatc gtcgatctcg acctgcccga gcttgaggta gatggcgtcg agcgcgcgct
      241 tgcccatgcc gggcacggcg cgcgtgtcga gcgtcagcac gagaaagccg aactcgcaga
      301 gcgcgttggg ggggacgaac gtctcgcgcg ccgccgccga cgccggtccg ccgtacaccg
      361 cggccagggc cgggtacttc gccgacgggt cgaagttcga cgggaagctg acgagaccgc
      421 gcaggagcgt cttgccgtcc gccgcgagat aggtgaacat ctcggccttc ctcaggccca
      481 gcgcggcgaa cttcgtcgtg tcgctcgccg ccagctccga cacgactctc cccgtgacgg
      541 cgtcgatgac gcgcagcgcc ggcggcgtgt catgcgtctg gtagacgtcc acggcgagcg
      601 tgctgtcggc ggaaatgccg caggcagccg ggccgccggc cggcgggccg ccgccccggc
      661 cgccgccgcc gggcaggcag ctgccgacgg tgtggtggaa cgccggatcg gtcagtcgca
      721 cgtccgtccg cccgtcgagg ccaacgcggt gcagctgcag cttcaggaaa ttgtcgccgt
      781 cccgcgccat caggaagacc acctgcgcgt cctcgtccag cttgacgagg ttcgcggcct
      841 cgaacgtcgt gtagcgcgtg agcggagtga tgaggcgccc ggacaggtcg tacaggtagt
      901 agttctgcca gcccgaccgc tcagacgccc agatgaaccg ccggccgtcc ttgaggaata
      961 ccgtcgcagg gctcgtctgg acccagcctg tcggccattc ctcgcgcacg accacgcggc
     1021 aggcccctgt cacgggatcg gcggccgcca gctcgagcac gttctgcctg cgattcgccc
     1081 ggagaaacag cagctccttc ccgtccggcg accacgccac gcggtagacg tagtgcccga
     1141 cgacgtcgtt ggtgaagggc ttgccgtccc ggacgtcgat gcgcacgctc gacttcccgg
     1201 cgaggtcgta cacgtacaga tcgacgaccg ggttcggctc gccggccttc ggataggcct
     1261 cgacgtccgc ggtgctgtag agcttcgtct ggtcgagctg gagaacgtag tcgggcacgc
     1321 ggctctcgtc gaagcggtag tacgcgagcc tccgaccgtc cggcgaccac cacatcgccg
     1381 tcgtctgggc gagttcctcg ccgtagaccc agctcgcggt tccggacttg atgcggcccg
     1441 tctcgctgcc gtcggtggtg acggcgacct cgccgccgcc cgaggcatcg ccgatgaaga
     1501 ggttccggtc gcggtagaac gccaccagcg tcttgtcggg cgactccgct gcggtcgcct
     1561 gccgcccgcg cgccacggcg ccgcccaggc cgcggcgccc gagcggcggc ggcgccggcg
     1621 ccccggcgtc aggcggaagc tccgacaccg cgcgcgtggc ggcgtcgaag cggaacatcc
     1681 tgccgtcctt cctgaagtcc agcgacctgc cgtcgtctct ccaggacgcc tggggcgcgc
     1741 cggactggac ggcgcccggg atctcccggc tcgtgcgctg gtactgctcg taccccggca
     1801 tggccttgag gcggtcctgg gcgacggcgg acgccgtcag cgcgacggcg gcgaacgcga
     1861 agaaggcggc gcagagtgcg aggcgggtgg tgttcatgcg gcttgggact ccggaagagg
     1921 ccgcacggct ggcgccgcgc ggtcgctcgc ttatatcaca gcaacgcgga cgccggggcc
     1981 gccgccgggg ggcggcgccg tgcgggcgcc gctcaccgcg gttgacgctg ctcccggaac
     2041 cgcactagtg tgctgcgaac acgcgcggcc ggaggctgcg caggacgccc cggcggcgag
     2101 ccgtcgtctg ccggcgatgg atcgaaaggc gcgccgatga cacagctgga ctggatcgtc
     2161 atcgtcttct acttctgcct catcggctgg gtcgcctggt attacggacg acaccagaag
     2221 gacaccaccg actacttcct cgcggggcgg aacgccggct gggtcgtgat cggcgcctcc
     2281 atcttcacct ctaacatcgg ctccgagcac atcgtggggc tggccggcca gggcgccacg
     2341 acgggcatgg gcatggccca ctgggaactg cacgcgtggg tgctcatcat gctcgccggg
     2401 ctgttcgtgc ccttctacta caagtccggc gtcgagacga tcccgcagtt cctggagaag
     2461 cggttcaacg cccgcgcccg gtggatcctg tcctgcgtgt cgctggtcgc gtacgtgttc
     2521 acgaaggtga gcgtcacggt gtacgccggc gcggtcgtgt tcaaggccct gttgccggaa
     2581 gcgttcggct ccccggagaa cgcgttctgg gtcggggcct tcaccacggt gatcctgacc
     2641 ggcatctaca cggtcttcgg cggcatgcgg gccatcatga acaccgccac gccgcaggcg
     2701 gtgatcatcc tgctgggatc gttcatcgtg acggcggtcg gtctcggaag gctcgggggc
     2761 tggggcgaac tggtgaccat ggcgcgggtg aaccgcgagg cgttcgcgct ctggcggccg
     2821 ctgtcggacc ccacgttccc ctggctggcc gtcgtcatcg cctcgccgat cgtgggcatc
     2881 tggtactggt gcacggatca gtacatcgtc cagcgcgccc tgtcggccag ggacctcgcg
     2941 acggcgcgcc gcggggcgct gttcggcggc gtgctgaagg tgtggcccgt gctcatcttc
     3001 gtgatcccgg gcctgatcgg ctgggcgctg caccagaagg gcctgctcct gctgccgact
     3061 acgctcgtgg acggcgtcgc ggtgatcaac cccgacctcg tcttcgcgac gctcgtgcgc
     3121 gagctgctgc cgtcgggcct gcgcgggctg atcatcgcct gcctgctcgc ggcgctgatg
     3181 agctcgctgg cgtcgctctt caactcgagc gcctcgctct tcacggtgga cgtgtacgag
     3241 aagctccgcc cgggccgctc ggagcggcac ctgcttcgcg tggggcgcat tgccaccgcg
     3301 acggtggtcg tgctgggcat cctctggatt cccgtgatgc agaaggtgca gggcggcggc
     3361 ctctaccagt accttcagaa cgtccagggc tacatcgcgc cgcccatcac ggcggtgttc
     3421 ctgctggggc tcttctgg
//
LOCUS       DAOSIF010000338         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24012_length_3435_cov_3.643491, whole genome shotgun sequence.
ACCESSION   DAOSIF010000338 DAOSIF010000000
VERSION     DAOSIF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24012_length_3435_cov_3.643491"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PLT35_10670"
     CDS             <1..903
                     /locus_tag="PLT35_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29728.1"
                     /translation="RAMVVAATEGRLARASRLSRLAEQLGWAIRQSHPDAVDPDCLRN
                     AANSFFYRQPESLPPMAPASSLSSEPHSFSRVFTAAWLESLAGMFDAGAPTSSRLAVV
                     AADAGKLLVSAVAHARITSSYFAQVAAGLLAADADLCDGRYRAAIQRAFVGRGILSMA
                     SAASLRRGAARRPSGTAARKGGDRLRPIEVAVDGRAYGLRVRRLLVEAPLGPSRESAA
                     SAAPDLGSMPAAEPHVAGRAFVEDLLRRGRVDVGAAAAGGAAAAGAVGDRRAARTTHA
                     LVGHGRTVTLVRRRFHDDAGAAGA"
     gene            1274..2803
                     /gene="metG"
                     /locus_tag="PLT35_10675"
     CDS             1274..2803
                     /gene="metG"
                     /locus_tag="PLT35_10675"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOG29729.1"
                     /translation="MSKFFLTTAIDYVNSRPHLGTAYEKITADVIARSRRLAGVPTHF
                     LMGNDEHSLNVFRRAKEEGLDPKAYCDRMELEFRAVWARLDVSFDDFIRTTEDRHRAG
                     VTALVARIQAAGDIYEGHYEGWYCNSCEAFKQEKDLEGGLCPTHRTKPQWIREKNHFF
                     RLSKYQQPLLDHYARHPEALEPEIRRNEILNIIQGGLEDISISRAGQAWGIPLPFDPQ
                     SVVYVWFDALINYISALGFGGPDEAAFERWWPASLHVIGKDITRFHCVIWPAMLMSAG
                     LPLPERVFGHGFVYFKGEKMSKSLGTIVDPLDAASKFGPDPLRLYLVREFAYGQDGDF
                     SWERFEERYNSDLANNLGNLVSRVATMADKYCGGRLPRPSAGPGRLAAVAADSVAAYR
                     AAMESFALQDCAGAAFAVVDAANAFIAETEPWKLAKDPASAARLAQILYEAAEAVRIA
                     AVLLLPVMPQSAAEILRRVGAPKPAADHRLDDAEWGAAGDLSTIKGDALWPRIGGTKG
                     N"
     gene            2850..3296
                     /locus_tag="PLT35_10680"
     CDS             2850..3296
                     /locus_tag="PLT35_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HOG29730.1"
                     /translation="MHDPYNLSRFVRAQEDDYPKALSELRRGRKRTHWMWFIFPQIEG
                     LGDSPTSREYSIKSLAEARAYLAHPVLGPRLIECAEAVLGVEGRSAAEILGFPDDLKL
                     RSSATLFARVAPSGSVFESILQKYFAGGPDAATLRLLAEMAGEREP"
BASE COUNT          484 a         1231 c         1240 g          480 t
ORIGIN      
        1 cgggcgatgg tggtggcggc caccgagggg cggctcgcgc gcgcctcgcg actgtcgcgg
       61 ctggcggagc agctgggctg ggcgatccgg cagtcgcacc ccgacgccgt cgatcccgac
      121 tgcctgcgca acgccgcgaa ctcgttcttc taccgccagc cggagagcct gccgccgatg
      181 gcgccggcca gctcgctgtc gtccgagccg cactcgttca gccgggtctt cacggcggcc
      241 tggctcgagt cgctggccgg gatgttcgac gccggcgctc ccacttcgtc gcggctggcg
      301 gtggtggcgg ccgatgccgg gaagctcctc gtgtcggccg tcgcgcacgc ccgcatcacc
      361 tcgagctact tcgcccaggt tgccgccggg ctgctggccg ccgacgccga cctctgcgac
      421 ggccgctacc gcgcggccat tcagcgggcg ttcgtggggc gaggcatcct ctccatggca
      481 tcggcggcgt cgctgcggcg cggcgcggcg cgccggccat ctggcacggc ggccaggaag
      541 ggaggcgacc gcctgcgccc aatcgaagtg gcggttgacg gccgcgcgta cggcctgcgg
      601 gtgaggcgcc tgctcgtcga ggcgcccctc gggccgtcgc gggagtcggc cgcgtcggct
      661 gccccggacc tcggctccat gccggcggcc gagccccacg tcgccggccg cgcgttcgtc
      721 gaggacctgc tcaggcgcgg ccgggtcgat gtcggagcgg cggctgccgg cggcgctgcc
      781 gcggcgggcg ccgtcggcga ccggcgtgcc gcccgcacga cgcacgcgct ggtcgggcac
      841 ggccggacgg tcacgctcgt ccggcgccgt ttccacgacg acgcgggtgc agccggcgcg
      901 taggggagca ggcctgccgg gccgttctcg tggagaccgg gtctcggggc caggccctag
      961 gtttctccga gcgcgcaccc gccgggccgg cccgcgtgcg ggcagcccgc gcaggcgggg
     1021 ccggacggtg gagaggcccc tcgcggcggc gccgagccca ccgcgaaggc ggacagctgc
     1081 ttctggacgc gggggctgcc gcatgccggg caggccggga cggtcccttc acgcacgagg
     1141 agttcgaagc gcgttccgca gccgtcacac gcgtactcgt acagcggcat ggcctcagta
     1201 tagcccggcc cccgcgcggg acgcccgccc gacctgatac catgatccgg tcgacgttca
     1261 gccgggaccc cgtatgtcca agttcttcct caccaccgcc atcgactacg tgaacagccg
     1321 cccccacctc gggacggcct acgagaagat caccgcggac gtcatcgccc gctcccgccg
     1381 gctggccggc gtgcccacgc acttcctcat gggcaacgac gagcattcac tgaacgtctt
     1441 ccgccgggca aaggaggagg gcctcgatcc gaaggcctat tgcgaccgga tggagctgga
     1501 gttccgggcg gtgtgggcgc gcctcgacgt gtcgttcgac gacttcatcc ggacgaccga
     1561 ggaccggcac cgcgccggcg tgacggcgct cgtggcgcgc atccaggccg cgggcgacat
     1621 ctacgagggg cactacgagg gctggtactg caactcctgc gaggcgttca agcaggagaa
     1681 ggacctcgag ggcggcctgt gcccgacgca caggacgaag ccgcagtgga tccgcgagaa
     1741 aaaccacttc ttccgcctct cgaagtacca gcagccgctc ctcgaccact acgcgcgcca
     1801 tcccgaggcg ctggagcccg agatccggcg gaacgagatc ctgaacatca tccagggcgg
     1861 cctcgaggac atctccatca gccgcgcggg ccaggcgtgg ggcatcccgc tgcccttcga
     1921 tccgcagagc gtcgtctacg tctggttcga cgcgctgatc aactacatct cggcgctcgg
     1981 gttcggcggc ccggacgagg cggcgttcga gcggtggtgg ccggcctcgc tgcacgtgat
     2041 tggcaaggac atcacgcgct tccactgcgt catctggccg gcgatgctca tgagcgcggg
     2101 cctgccgctg cccgagcgcg tcttcggcca cgggttcgtc tacttcaagg gcgagaagat
     2161 gagcaagtcg ctgggcacga tcgtcgaccc cctggacgcg gcgtcgaagt tcggccccga
     2221 tccgcttcgc ctctacctcg tgcgcgagtt cgcgtacggc caggacgggg acttctcgtg
     2281 ggagcgcttc gaggagcgct acaattccga cctcgcgaac aacctcggca acctcgtcag
     2341 ccgcgtcgcg accatggccg acaagtactg cggcggcagg ctgccccggc cctccgcagg
     2401 gcccgggcgg ctggcggccg ttgcggccga cagcgtcgcg gcctaccgcg cggcgatgga
     2461 gtcgttcgcg ctgcaggact gcgccggcgc cgccttcgcc gtcgtcgatg cggccaacgc
     2521 cttcatcgcc gagaccgagc cgtggaagct ggcgaaggac ccggcgagcg cggcgcgcct
     2581 cgcccagatc ctctacgagg cggccgaagc cgtccgcatc gcggccgtgc tcctgctgcc
     2641 ggtcatgccg cagtcggcgg ccgagatcct caggcgcgtg ggcgcgccca agcccgccgc
     2701 cgaccatcgc ctggacgatg ccgagtgggg cgcggccggc gacctgtcca cgatcaaggg
     2761 cgacgcgctg tggccgagga tcggggggac gaaagggaac tgatgtcggg ccccggcgtc
     2821 cgaaacccgg agtgagtccg gagggcggca tgcacgaccc gtacaatctg agccggttcg
     2881 tgcgggcgca ggaagacgac tacccgaagg ccctgtcaga gctcaggcgc ggccggaagc
     2941 gcacgcactg gatgtggttc atcttcccgc agatcgaagg gctaggagac agcccgacgt
     3001 cgcgggagta ctcgatcaag agcctcgcgg aggcccgcgc ctatctggcc catcccgtcc
     3061 tcggccccag gctgatcgag tgcgccgagg cggtcctcgg ggtggaggga cgatctgccg
     3121 cggagatcct cggttttccc gacgacctga agctgcgatc gtcagccacg ctcttcgcgc
     3181 gcgtcgcgcc cagcggctcg gtcttcgaga gcatccttca gaagtacttc gccggcgggc
     3241 ccgacgcagc caccctccgc ctgctggcgg agatggcggg ggaacgggaa ccgtgacggc
     3301 ggagcggcgt tcttcggcga ccgctgcgag cgcgatcccg gatcgtgcgc cggtacgatc
     3361 gtccaccggc cggcgacggc ctggtgggtg cagatcagga acgaacaggg acaggccggc
     3421 tggaccagcg agccg
//
LOCUS       DAOSIF010000339         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24024_length_3434_cov_3.300385, whole genome shotgun sequence.
ACCESSION   DAOSIF010000339 DAOSIF010000000
VERSION     DAOSIF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24024_length_3434_cov_3.300385"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLT35_10685"
     CDS             complement(<1..965)
                     /locus_tag="PLT35_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG29731.1"
                     /translation="MPAPAPTRRARRRVGLALGGGGVRGLAHIGVLQVLAEAGVPISA
                     IAGVSMGAIVGAAYALTDEFPGALIAALRGLDISPFKSLPRGAGSDGLWTVVRRLVDV
                     ERFMISNVLSWGVASDAKVAALASQLAGERRLEDARVPLGIVACDLRSGKPVVFRSGP
                     ATLALQASSALPGFFKPVEFEGRLLADGGYAGVVPTYVVRAMGAEAVVAVDADPARAV
                     EDIHDGLHALFRAFDITSQRLKEALLRDAEVVIRPDFGGSIDTLDFSQADRCVQAGAS
                     AARAALPAIAALQARPRRARWWRRRTSTSTPRAPRARGVDWPGTPV"
     gene            complement(1093..2340)
                     /locus_tag="PLT35_10690"
     CDS             complement(1093..2340)
                     /locus_tag="PLT35_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29732.1"
                     /translation="MAERTLTYGFVGAGFNAKFHLAALRQLRGIEVAGVVSVAGAAEF
                     AALARGWGLGSTQVYGSVGEMAKHVECVAVCAPNDARIAIVEAIADAVKAGAELKGVI
                     CEKPLGRNVAEARRLVELAEGAGLRTAYFENQIHMKPIRVQLAQLEPLRRQMGPLALA
                     RSAEEHGGPHEGWFWDPTRQGGGVLCDMGCHSIAVGWYVLTPTGKPLTFLEPQEVTAD
                     LGLLKWGQPAWREKLLKERGVDYAKTPAEDFATGMITFRNPETGQRVKAQFTNSWMFE
                     KQGLRLLMDGMGPGYAFEVNSLLSPLTIFVGDAAAEAIKDAELALEKATASRGLLAVQ
                     HNEPDLYGYTDENADARDAFLEGRDAALTWRYGLEITTLVMAAYLSAEQKRVVDLTNP
                     ATQRALETYVPLIQQGRGAEQLY"
     gene            complement(2363..>3434)
                     /locus_tag="PLT35_10695"
     CDS             complement(2363..>3434)
                     /locus_tag="PLT35_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG29733.1"
                     /translation="YGKALQIENIGNLTFALFLTSCIVQLVIGPLVDRFGYRPVAIAG
                     FLVAAVSMLLLAVAASYATALVACILLGVGAMCMNTVGNTLIPVVLFGGKDPARASNF
                     GNAFFGLGLVVTPLLFYVVNYKAGLFVLTAVFAAFLAAAVGTRFPQAASGYRFSMAVK
                     LLGNPAVLVAALALFCYISLEASMNTWSKPYMTELLGGDQNPTAVSKASLVLSLFGVA
                     MMAGRFLTSAIKNLTNMGVKLIATMAAVAAASILTLILTSSAALAVVAVILTGLAFAP
                     IFPTIVGVTFAKFDSSLYGSIFGIIFAIGLLGGTFVPQIVGRLSQGATIQQSLQIVLA
                     MAVALFVASWLLKTGSKRGGAR"
BASE COUNT          538 a         1241 c         1172 g          483 t
ORIGIN      
        1 accggtgtac ctggccaatc aacgcctctc gctcgcggag ctcgaggcgt cgacgtcgag
       61 gtacgccgtc gccaccagcg cgcgcggcga ggccgcgcct ggagggcggc gatggccggc
      121 agggccgccc gcgcggcgct ggcgccggcc tgcacgcatc ggtcggcctg cgagaaatcg
      181 agcgtgtcaa tcgatccgcc gaagtccggc cggatgacga cctcggcgtc gcgcaacagg
      241 gcttccttga gtcgctgaga cgtaatgtcg aacgcccgga acagcgcgtg gaggccgtcg
      301 tggatgtcct cgacggcgcg tgcggggtcc gcatcgacgg cgacgacggc ctcggcgccc
      361 atcgcccgga cgacgtaggt cggcacgacg cccgcgtagc ctccgtccgc gagcaaccgc
      421 ccctcgaact cgactggctt gaagaagccc ggcaacgccg aactcgcctg gagcgcgagc
      481 gtcgccggcc ctgagcgaaa cacgacgggc ttgcccgagc gcaggtcgca ggccacgatg
      541 ccgagcggca cgcgggcgtc ttccagccgc cgctcgccgg ccagctggga cgcgagcgca
      601 gccaccttgg catcggacgc aacgccccag gagaggacgt tgctgatcat gaagcgctcg
      661 acgtcgacga ggcggcgcac gaccgtccac aggccgtcgg atcccgcccc cctcgggagc
      721 gacttgaacg ggctgatgtc aaggccgcgc agggccgcga tcagggcgcc cgggaactcg
      781 tccgtcagcg cgtatgcggc cccgacgatc gcgcccatgc tgacgccggc gatcgcggag
      841 atcggcacgc cggcttcggc gaggacctgg agcacgccga tgtgcgcgag gccgcgcacg
      901 ccgccgccgc cgagggcgag gccgacgcgg cgccgggcac gacgggtcgg cgccggagcg
      961 ggcatgattc gcacgacacc ctccgggggc cgccggtcct gccgggtatt ctagccgaaa
     1021 cgatgccccc tccccgatcc ggtctggttc gcggcgaggg cctctcccct cacatcctcg
     1081 tggcaccggc gctcagtaca gctgctccgc gccccgtccc tgctggatga gcggcacgta
     1141 cgtctccagg gcccgctgcg tcgcgggatt ggtgagatcc acgacccgct tctgctcggc
     1201 ggacaggtac gcggccatca ccagcgtggt gatctcgagg ccgtagcgcc acgtcagcgc
     1261 ggcgtcgcgc ccctcgagga acgcgtcgcg cgcgtcggcg ttctcgtcgg tgtagccgta
     1321 gagatcgggc tcgttgtgct ggaccgccag cagcccccgc gacgccgtgg ccttctccag
     1381 cgcgagctcg gcgtccttga tcgcctccgc cgcggcgtcg ccgacgaaga tcgtcagcgg
     1441 cgacaggagc gaattgacct cgaacgcgta gcccggcccc atgccgtcca tgagcaggcg
     1501 cagcccctgc ttctcgaaca tccacgagtt ggtgaactgc gccttcacgc gctggccggt
     1561 ctccggattg cgaaacgtga tcatgccggt ggcgaagtcc tccgccggcg ttttcgcata
     1621 gtccacgccc cgctccttca ggagcttctc gcgccaggcg ggctggcccc acttgagcag
     1681 cccgaggtcg gcggtgacct cctgcggctc gaggaacgtc aggggcttgc cggtcggcgt
     1741 caggacgtac cagccgacgg cgatgctgtg gcagcccatg tcacacagca cgccgccgcc
     1801 ctgccgggtc gggtcccaga accacccctc gtgcggcccg ccgtgttcct cggccgagcg
     1861 ggccagcgcg agcggcccca tttgccgcct gagcggctcg agttgcgcga gctggacccg
     1921 gatgggcttc atgtgaatct ggttctcgaa gtacgcggtt cgcagtccgg ccccctctgc
     1981 cagctcgacg agccgccgcg cctcggcgac gttgcggccg aggggcttct cgcagatgac
     2041 gcctttcagc tcggcgcccg ccttcacggc gtcggcgatg gcctcgacga tggcgatccg
     2101 cgcgtcgttg ggggcgcaga cggcgacgca ctcgacgtgc ttcgccatct cgcccacgct
     2161 gccgtacacc tgcgtgctgc ccagccccca gccgcgcgcc agcgccgcga actcggccgc
     2221 gccggcgacc gacacgacgc cagcgacctc gatcccgcgg agctgccgca gggccgccag
     2281 gtggaacttc gcgttgaatc cggctccgac gaagccgtag gtcagggtcc gctctgccat
     2341 gtccgcctcc gtgccgccgg gcctaccggg ccccgccgcg cttcgatccc gtcttcagca
     2401 gccacgacgc cacgaacagc gccacggcca tcgccagcac gatctggagg ctctgctgga
     2461 tggtcgcgcc ctggctgagg cggcccacga tctgcggcac gaacgtgccg ccgagcaggc
     2521 cgatggcgaa gatgatgccg aagatgctgc cgtaaaggct ggagtcgaac ttggcgaacg
     2581 tcacgccgac gatcgtcggg aagatcggcg cgaacgccag cccggtcagg atcacggcca
     2641 cgacggccag tgccgcgctg gacgtcagga tcagcgtcag gatggaggct gccgccaccg
     2701 ccgccatggt ggcaatgagc ttcacgccca tgttcgtcag gttcttgatc gccgacgtca
     2761 ggaaccggcc ggccatcatc gccacgccga acaggctcag gacgaggctg gccttggaga
     2821 cggcggtcgg gttctggtcg cccccgagga gctccgtcat gtagggcttg ctccaggtgt
     2881 tcatgctcgc ctcgagcgag atgtagcaga acagcgccag cgccgccacg agcaccgcgg
     2941 gattcccgag cagcttcacc gccatcgaga acctgtagcc actcgccgct tgcgggaatc
     3001 tcgtgccgac ggctgccgcc aggaacgccg cgaacaccgc ggtcagcacg aagaggccgg
     3061 ccttgtagtt gaccacgtag aacagcagcg gcgtgaccac caggccgagg ccgaagaagg
     3121 cgttgccgaa gttgctcgcc cgggcaggat ccttgccgcc gaagagcacc accgggatga
     3181 gcgtgttgcc gacggtgttc atgcacatcg cgccgacgcc cagcaggatg caggcgacga
     3241 gcgccgtcgc gtacgacgcc gcgaccgcca gcaggagcat gctgacggcc gccacgagga
     3301 acccggcaat cgccaccggc cggtagccga accggtccac cagcgggccg atgacgagct
     3361 gcacgatgca gctggtgagg aacagcgcga acgtcagatt gccgatgttc tcgatctgga
     3421 gggccttccc gtac
//
LOCUS       DAOSIF010000340         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24103_length_3425_cov_3.772404, whole genome shotgun sequence.
ACCESSION   DAOSIF010000340 DAOSIF010000000
VERSION     DAOSIF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24103_length_3425_cov_3.772404"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLT35_10700"
     CDS             <1..454
                     /locus_tag="PLT35_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG29734.1"
                     /translation="VGAHTDSPGFKLKPEPSAYAFGWSQAGMEVYGGPLLNSWLDREF
                     GLAGRVTPPAVLPGRGHVFNQYVVRVPDRDRVKAHLEERRVGAAVYYPVPFHQLTCFA
                     SLGYRAGQFPHAERAARETLALPVYAELTADEQRYVVEVVAEALERAA"
     gene            476..1363
                     /locus_tag="PLT35_10705"
     CDS             476..1363
                     /locus_tag="PLT35_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG29735.1"
                     /translation="MAGCRVVVTGAAGRLGRAMCAEFGRDASVAALTRTDLDITDGRA
                     VGARLAAERPDIIVNCAGDNDVDLAEGRPVRALEVNAFAVKALARAADEAGAALVHFS
                     SDFVFDGEASRPYTEADEPNPRSVYAASKLLGEWFALEARRSFVLRVESLFGPAGAGR
                     VGSLDRIVEGIRAGAPVPVFVDRTVSPAYVADVAWATRRLIEGGAPGGLYHCVNAGAA
                     TWREIALEIARLLGREPRLTPTTLDQAALRASRPRYSALDISKLGAAGIVMPGWQTAL
                     ARYLGSDPEKRGQAETLEA"
     gene            complement(1389..2474)
                     /locus_tag="PLT35_10710"
     CDS             complement(1389..2474)
                     /locus_tag="PLT35_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG29736.1"
                     /translation="MEEIDAVIVGGGVTGLASAAAIAEGGASVCVLERHPRPGMDAST
                     HNSGVIHAGIYYPPGSLKARLSVEGARELYAFCARRGVPHARCGKLIVAVDRQEAERL
                     PDLEARARANGVEKLAIVDSGAIRRREPRAAGVAALLSPDTGVVEPEALVLALGRACA
                     NAGAHVLTGTSLIGADESSRGLIVRTERESILCQTVVNAGGVFADHVSAMLGGESFAI
                     HPCRGEYAELAPARSGAVEALVYPLPDASGHGLGIHLTKTTRGRVLIGPTVRHQQSRH
                     DYESGRMALEGFLAPARRLLPELELKDLRLGGSGIRANLNPPDEPFADFLIRRDRLNP
                     RIIHAAGISSPGLTACLAIGRLVASLI"
     gene            2580..>3425
                     /locus_tag="PLT35_10715"
     CDS             2580..>3425
                     /locus_tag="PLT35_10715"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG29737.1"
                     /translation="MPKRAIITGITGQDGSYLAELLLEKGYEVTGITRRLSQPNLDRI
                     AHLLDRIKLYPADLLDVLSLIHAIEENEPDEFYNLAAMSFVPASWDQPLLTGEFNAQG
                     VTRVLDAIRRVNPKIRFYQASSSEMFGKVREVPQSELTPFHPRSPYGVSKVFGHYITV
                     NYRESYNLFACSGLLFNHESPRRGLEFVTRKVSDGVARIKLGLQDTLLLGNLEAHRDW
                     GFAGDYVRAMWLMLQQPTPDDFVVATGVSHSVKDLVRVAFAHAGLDWEKHVRVDPRFL
                     RPAEVD"
BASE COUNT          484 a         1266 c         1189 g          486 t
ORIGIN      
        1 cgtcggggcg cacaccgatt cacccgggtt caagctcaag ccggagccgt ccgcctacgc
       61 gttcggttgg tcgcaggccg gcatggaggt ctacggcggg ccgctgctga actcctggct
      121 cgaccgcgag ttcggcctgg ccgggcgcgt gacgccgccc gcggtgctgc ccgggcgcgg
      181 gcacgtcttc aatcagtacg tcgtgcgcgt gccggaccgc gaccgggtca aggcgcacct
      241 cgaggagcgg cgcgtcggcg cggccgtcta ctacccggtg ccgttccacc agctgacctg
      301 cttcgcgtcc ctcgggtacc gcgcagggca gttcccgcat gccgagcggg ccgcgcgcga
      361 gacgctcgcg ctgccggtct acgccgagct cacggccgac gagcagcgct acgtcgtcga
      421 agtcgtcgcc gaggcgctgg agcgggcggc ctgaatggcg gcgggaggcg ggcgcgtggc
      481 cggctgccgg gtggtcgtca cgggcgcggc gggccggctg ggccgggcca tgtgcgccga
      541 gttcggccgg gacgcctccg ttgccgccct cacgcgcacc gacctcgaca tcaccgacgg
      601 ccgcgcggtc ggcgcccggc tggccgccga gcgcccggac atcatcgtca actgcgccgg
      661 cgacaacgac gtcgacctcg ccgagggccg gcccgtgcgg gcgctcgagg tgaacgcgtt
      721 cgccgtgaaa gcgctggcgc gcgccgcaga cgaggccggg gccgcgctcg tgcacttcag
      781 cagcgacttc gtgttcgacg gcgaggcctc gcgcccgtac acggaagccg acgagccgaa
      841 tccgcggagc gtctacgccg cctccaagct gctcggcgag tggttcgcgc tcgaggcgcg
      901 ccgctcgttc gtgctgaggg tcgagagcct gttcggcccc gccggcgcgg gccgggtcgg
      961 cagcctcgat cgcatcgtgg agggcatccg ggcgggcgcg ccagttcccg tgttcgtcga
     1021 ccgcacggtg tcgccggcct acgtggcgga cgtggcgtgg gcgacgcgcc gcctgatcga
     1081 ggggggcgcc ccgggcgggc tgtatcactg cgtgaacgcc ggcgcagcca cgtggcgcga
     1141 gatcgccctg gagatcgcgc ggctactggg ccgggagccg cggctcacgc cgaccacctt
     1201 ggaccaggcc gcgctccggg ccagccggcc tcgctactcg gccctcgaca tctcgaaact
     1261 cggcgccgcc ggcatcgtga tgccgggatg gcagaccgcc ctcgccaggt atttggggtc
     1321 agatccagaa aaacggggtc aggccgagac gctcgaggcc tgaccccgtt tttctggatc
     1381 tgaccccatc agatgagcga agccacgagg cggccgatgg cgaggcaggc cgtcaatccg
     1441 ggggaactga tgccggccgc gtgaataatc cgcggattca gccggtcgcg gcggatcagg
     1501 aagtccgcga acggctcgtc cggcggattc aggttcgccc ggatcccgct ccctcccagg
     1561 cggagatcct tcagttcgag ctccggaagc agccgccgcg ccggcgccag gaacccctcg
     1621 agcgccatcc ggccggactc gtagtcgtgc cggctctgtt ggtgccggac cgtgggccca
     1681 atcagcacgc ggccccgcgt ggtcttcgtc aggtggatcc ccagcccgtg gccggaggcg
     1741 tcgggcagcg ggtacaccag cgcctcgacc gcgccgcttc gcgccggcgc gagctccgcg
     1801 tactcgccgc ggcagggatg gatggcgaac gactccccgc cgagcatcgc ggacacgtgg
     1861 tcggcaaaca cccctcccgc attgacgaca gtctggcaga ggatcgactc ccgctcggtc
     1921 cgcacgatca gcccgcgcga ggactcgtcg gcgccgatca ggctcgtccc ggtcagcacg
     1981 tgcgcccctg cgttcgcgca ggcccggccg agcgcgagga ccagcgcctc gggctccacg
     2041 acgcccgtgt cgggcgacag gagcgccgcc acccccgcgg cccgcggttc ccggcggcga
     2101 atggccccgg aatccacgat cgcgagcttc tcgaccccgt tggcgcgggc ccgcgcctcc
     2161 aggtccggca gccgctccgc ctcttgccga tccacggcga cgatgagctt gccgcagcgg
     2221 gcgtgcggga cgccgcggcg ggcgcagaac gcatacagct cccgcgcgcc ctcgaccgac
     2281 aagcgcgcct tcagcgatcc gggggggtag tagatgccgg catgaatcac gccgctgttg
     2341 tgcgtgctcg cgtccatgcc gggcctggga tggcgttcca gcacgcacac ggacgcgccc
     2401 ccttcggcga tggccgctgc cgaggcgagg ccggtcacgc ccccgccgac gatgacggcg
     2461 tcgatctcct ccatggaggg ccctccccgg ccggctcccg gtgcggctgt cgagcgcctt
     2521 gcctgggcgc gagcgatcct actacgatgc ggtgttccac ccgatacgag gacgaagaca
     2581 tgcccaagcg cgccatcatc accggcatta caggccagga cgggtcgtac ctggccgaac
     2641 tgctgctcga gaaaggctac gaggtcaccg gcatcaccag gcgcctcagc cagcccaacc
     2701 tggaccggat cgcccacctg ctggaccgca tcaagctcta ccccgccgac ctgctggacg
     2761 tgctctcgct catccacgcc atcgaggaga acgagccgga cgagttctac aacctggcgg
     2821 cgatgtcgtt cgtgcccgcc tcctgggacc agccgctgct gaccggcgag ttcaacgccc
     2881 agggcgtgac ccgcgtgctg gacgccatcc gccgcgtcaa cccgaagatc cggttctacc
     2941 aggcgtcgtc gagcgagatg ttcggcaagg tgcgcgaggt gccgcagtcg gagctgaccc
     3001 cgttccaccc gcggagcccc tacggcgtct cgaaggtctt cggccactac atcaccgtga
     3061 actaccgcga gagctataac ctcttcgcct gctccggcct cctcttcaac cacgagtcgc
     3121 cgcgccgggg gctggaattc gtcacccgca aggtgtcaga cggcgtcgcc cgcatcaagc
     3181 tcggcctcca ggacacgctg ctgctcggca atctcgaggc gcaccgcgac tggggcttcg
     3241 ccggcgacta cgtccgcgcg atgtggctca tgctccagca gcccacgccg gacgacttcg
     3301 tcgtcgccac cggcgtgagc cactcggtca aggacctcgt gcgggtggcc ttcgcccacg
     3361 ccggcctcga ctgggagaag cacgttcgcg tcgatccgag gttcctgcgc ccggccgagg
     3421 tcgac
//
LOCUS       DAOSIF010000341         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24183_length_3416_cov_3.461470, whole genome shotgun sequence.
ACCESSION   DAOSIF010000341 DAOSIF010000000
VERSION     DAOSIF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24183_length_3416_cov_3.461470"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1007)
                     /locus_tag="PLT35_10720"
     CDS             complement(174..1007)
                     /locus_tag="PLT35_10720"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOG29738.1"
                     /translation="MIRLVAIDIDGTLLDSRWRLPARNRDAVLACAARGVEVALVTGR
                     AYGFARPIVDLLPCPVTVIASNGALVRLSTGVTALRHTMPRAIARAVLAAGAAYRRNV
                     ALTFDRPRDGQMVFERVDRSDPVRLAYAERNIAYLSEVAPLESALTEDPLVVMFNGAV
                     AAMRQLAALLRELPEAPAFSVNLTEYEDRDISFVDVLTAGCTKGATLAEWARRQGYAR
                     GEVMALGDNLNDVGMLEAAGLPVVMGNAVEALRARGWHITASNDEAGVADAIDRFVLT
                     R"
     gene            1181..1948
                     /locus_tag="PLT35_10725"
     CDS             1181..1948
                     /locus_tag="PLT35_10725"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOG29739.1"
                     /translation="MSRPLNRRLLLVFAHPDDESVFAAGVVCRTVAGGGRVALCTATP
                     GEHGKLGDPPVCSREDLGRARQAELVEACRILGISTMRVLGYPDRALAVAPADTIRRQ
                     LVEVIRAVRPQVVATFDPNGTNLHPDHVAISRFAGDAVSAAADARWYPELGKPHTVAR
                     LAWTLPVRPWAVLRRGDPAPEPGVDFAIDVEAFARRKAAALRAHRSQHLTIDRIFFSK
                     PDRGRLLSVELFRQAFGPRLGSVPETDLFAGIAGRLS"
     gene            1962..>3416
                     /locus_tag="PLT35_10730"
     CDS             1962..>3416
                     /locus_tag="PLT35_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29740.1"
                     /translation="MRRAFAILTLSLSVLLGLAPLTARHAATIGAPAARAQVSAPSAE
                     PTGSGQSGAPSAAAAAAPRPMELPDILAWKSIGASAVSDDGRWFAYRLSPVVGDSEVV
                     VRAVEGDKAYTFPAGEAPSAGGRPGGPGDEPGLPAAALQFSADSKWAAFMVYPTRAEA
                     QRARSQRRPAAQPKARLLDLESGTDVAFDNVRRFAFAGERGGWIAMQKAPAGAGGTAV
                     GAPPAQGPARGGSGGDAAGDRPKGTDLILRELATGRDLNVGNVSEFAFDKSGRYLALV
                     IDAPDKAGNGVQVRDMETGVVQALDSASASYERLSWTEKGDGLSVIRGRDDERYRDKR
                     YAAIGFTGFGAAGALQRVVYDPASDEGFPQGFSISPARSAVWIEDLGALLFGIRELEK
                     AEPKPGATPADKGPADSPGAPAPPPAPETPDDDKVDLVLWHYKDPRLQSQQRVQEQAD
                     KNFSYLSIFRVKENRFIRLADDEVRSVSAAPKQRY"
BASE COUNT          465 a         1209 c         1244 g          498 t
ORIGIN      
        1 gtccctggcc ggcgtcctca acgatccgag ggcgctcgaa tcggtcatgc gcggctgggt
       61 cgaaccgaag acgccctgag accttccccg gccgcctgca cggcggcagg acgtcatgcg
      121 ggcaggccag ggggcgggcc gcttcgggtg atcggccttc gtcccgccgg gcgctaccgg
      181 gtcagcacga agcgatcgat tgcgtccgcg acgccggcct cgtcgttcga cgccgtgatg
      241 tgccagccgc gggcgcgtag cgcctccacg gcgttgccca tcacgaccgg caggccggcc
      301 gcctcgagca tgccgacgtc gttcaggttg tcgccgaggg ccatcacctc gccgcgcgcg
      361 tagccctggc gccgcgccca ctccgccagc gtcgcgccct tcgtgcagcc ggccgtcagc
      421 acgtccacga acgagatgtc gcggtcttcg tactcggtca ggttcaccga gaacgccggc
      481 gcctccggca actcccggag cagcgcggcg agctgccgca tcgccgcgac ggcgccgttg
      541 aacatgacga ccagcgggtc ctcggtgagc gccgactcga gcggcgccac ctcgctcaga
      601 tacgcgatgt tgcgctctgc gtacgcgagc cgcacggggt cgctgcggtc gacccgctcg
      661 aagaccatct ggccatcacg cggcctgtcg aacgtcagcg cgacgttgcg gcggtaggcg
      721 gcgccggctg cgagcaccgc ccgcgcgatc gcccggggca tcgtgtgccg aagcgcggtc
      781 acgcccgtcg agaggcggac gagcgcgccg ttactggcta tgacggtgac agggcaggga
      841 agcaggtcga cgatggggcg cgcgaaaccg tacgcgcggc cggttacgag ggcgacttcg
      901 acccctctcg cggcgcaggc gaggaccgca tcgcggttgc gagcgggcag ccgccaccgg
      961 gaatcgagca gcgtgccgtc gatgtcgatg gcgacgagac gaatcatcgg gacagtgtag
     1021 cgtaggcgct gggggcttgg aggctaggcg ctggtcgctc tcctggattc tgaatgctga
     1081 cttccggatt ccttcacctc ctttgcctcc cttgcctcct ctacctcctc gacgtcttct
     1141 gcctcctcgc ccccgcatgg taagctcgcc tgaattgccc atgtcccggc ctctcaaccg
     1201 ccgcctgctc ctcgtcttcg cgcatcccga cgacgagtcg gtgttcgccg ccggagtggt
     1261 gtgccggacg gtagccgggg gcgggcgcgt ggccctctgc acggcgacac ccggcgagca
     1321 cgggaagctg ggcgatccgc cggtctgttc gcgggaggac ctgggccgcg cccggcaggc
     1381 ggagctggtg gaagcgtgcc gcattctcgg catctcgacg atgcgggtcc tgggctaccc
     1441 ggatcgtgcg cttgccgtcg cgcccgcgga cacgatccgg cggcagcttg tcgaagtcat
     1501 ccgggccgtg cgcccccagg tggtggccac cttcgatccc aacggcacga acctccatcc
     1561 cgaccacgtg gccatcagcc gcttcgcggg cgatgccgtg agcgcggcgg cggacgcgcg
     1621 ctggtacccg gagcttggga agccgcacac cgtcgcgcgc ctcgcctgga ccttgcccgt
     1681 gcgcccgtgg gcggtcttgc ggcgcggcga tccggcgccg gagccgggtg tggacttcgc
     1741 gattgacgtc gaggcgttcg cgcgccggaa ggccgccgcg ctcagggcgc accggtcgca
     1801 gcacctcacc atcgaccgca tcttcttctc gaagccggat cgcgggcggc tgctgagtgt
     1861 cgaattgttt cgccaggcgt tcggcccgcg cctggggtcc gtgccggaga ccgacctgtt
     1921 cgccgggatc gccggcaggt tgtcgtgacc aggaggttgc catgcgtcgg gcgttcgcga
     1981 tactgaccct ctcgctgtcg gtcctgctgg ggctcgcgcc gttgacggcc cggcacgccg
     2041 cgacgatcgg cgcgcccgcg gcccgggcgc aggtttcggc gccgtcggcc gaaccgaccg
     2101 gttccgggca gtcgggtgcg ccgtctgccg cggcggctgc cgcgccgcga ccgatggaac
     2161 tgcccgacat cctggcctgg aaaagcatcg gcgccagcgc ggtgtccgac gatggccgct
     2221 ggttcgccta ccggctgtcg cccgtcgtcg gcgacagcga ggtcgtggtg cgggcggtcg
     2281 agggcgacaa ggcttacacg tttccggcgg gcgaggcgcc gtccgcggga gggcggccag
     2341 gagggcccgg agacgagccc ggcctgcccg cggccgcgct ccagttctcg gccgactcga
     2401 agtgggctgc gttcatggtc tacccgacgc gcgcagaggc tcagcgcgca cggagccagc
     2461 ggcggcccgc ggcgcagccg aaggcgcgcc tgctcgatct cgagtcggga acggacgtcg
     2521 cgttcgacaa cgtgcggcgc ttcgcgttcg cgggcgagcg cggcggatgg atcgccatgc
     2581 agaaggcgcc ggcgggcgcc ggcggaacgg cggttggtgc tcctccggcc cagggcccgg
     2641 ctcgcggggg atcgggaggc gatgcggccg gcgaccggcc gaagggcacg gacctcattt
     2701 tgcgcgagct tgcgacgggg cgcgacctga acgtcggcaa cgtctcggag ttcgccttcg
     2761 acaagtcggg gcgctacctg gccctcgtca tcgacgcgcc cgacaaggcc gggaacggcg
     2821 tccaggtgag ggacatggag accggcgtcg tgcaggcgct cgacagcgcc agcgcctcgt
     2881 acgagcgcct gtcgtggacc gagaagggcg acggcctgtc ggtcatccgg ggcagagacg
     2941 acgagcgata ccgcgacaag cggtacgcag ccatcggctt cacgggattc ggcgccgccg
     3001 gcgccctgca gcgcgtcgtt tacgatcccg cctccgacga ggggttcccg cagggcttct
     3061 cgatcagtcc ggcccgcagc gccgtctgga tcgaggatct cggcgccctc ctgttcggca
     3121 tccgggaact cgagaaggcg gagccgaagc ccggggcgac gccggccgac aaggggcccg
     3181 ccgacagccc gggcgcgccc gcgcctcctc cggcccccga gacgccggac gacgacaagg
     3241 tggacctggt cctgtggcac tacaaggacc cgcgcctcca gtcgcagcag cgggtgcagg
     3301 agcaggcgga caagaacttc agctacctct cgatcttccg cgtgaaggag aaccggttca
     3361 tccggctcgc cgacgacgag gtgcgcagcg tcagcgctgc gccgaagcag cggtac
//
LOCUS       DAOSIF010000342         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24442_length_3388_cov_3.039604, whole genome shotgun sequence.
ACCESSION   DAOSIF010000342 DAOSIF010000000
VERSION     DAOSIF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24442_length_3388_cov_3.039604"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLT35_10735"
     CDS             <1..773
                     /locus_tag="PLT35_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG29741.1"
                     /translation="SELFGHERGAFTGAVKDRPGRFELAQGGTIFLDDVDDVPMSMQV
                     KLLRVLQNRTIERLGGTRAIPVDVRVITGTKRPLKEVVAEGRFREDLFYRLNVIPINL
                     PPLRERREDVPILLEHFLRRFFAERGEEPRPIAPAVQQAFLRYPWPGNVRELENACER
                     IAQTCTCETVRLGCVPINVLFHRHVEEQSPVVEAHQEPTAISLEERLREVELNLISWA
                     LKASGGNKSRAAELLNVKRSTLGDRVKKLGLEKLQARD"
     gene            complement(1064..2089)
                     /locus_tag="PLT35_10740"
     CDS             complement(1064..2089)
                     /locus_tag="PLT35_10740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG29742.1"
                     /translation="MKKSAVKRSTAGSPRKPQPPVRAAKAATATAAQAESASTKASPA
                     PAAVAPEPPAAASKAAARVRVVRPAATATLMALHEGIVYGPVRSRRLGRSLGINLTPA
                     HLKLCTFNCSYCQYGWSAHSRRTATPAFEHWPSPSAVAKAVASAIRALSAQGERLDRL
                     TLAGHGEPTLHPKFREVVRALRAVRDDLAPGVPLAVLSNASTLEQAEVREALAALDER
                     YMKLDAGDTATLRTVNGATMTIEQVIAGLKKVPDIVIQSMFVRDRTGRIDNTGDLTVI
                     NWVGALDRIRPKAVQVYTIDRIPAFPYLQQVPAARLLEIAQRVRLAGFRCDVYGVPEA
                     LAAPDKA"
     gene            complement(2518..>3388)
                     /locus_tag="PLT35_10745"
     CDS             complement(2518..>3388)
                     /locus_tag="PLT35_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOG29743.1"
                     /translation="WAVTLPVAHGDVPHWGEPLLDRELAALYSTSGEAPPLLQSNCNL
                     CHRYERATEGAGAINLGKSLVRQKGCRSCHAVNGRGGAVGPDLTWVGDKSAEQFDYSR
                     LRGRPTVFAWHLAHLKDPRLVVADTLMPNFHLSVRECQALALLVMSWRRVAFGAAYLP
                     GTPAEDLPSAEEQLAETRLRSGRGGWFAQAGCAVCHSVAVLGMTSPTPIGPDLSTAAD
                     DTQKRFGVTLERYLATPSGTMAAVLSRQILLSPAQREAAAQHLRQAFREYQRIEAEGG
                     TPLALPGGVGLER"
BASE COUNT          476 a         1192 c         1224 g          496 t
ORIGIN      
        1 agtccgagct cttcggccac gagcgcggcg cgttcaccgg cgccgtgaag gatcggcccg
       61 gtcggttcga gctggcgcag ggcggcacga tctttctcga cgacgtcgac gacgtgccga
      121 tgagcatgca ggtgaagctc ctgcgcgtcc tgcagaaccg caccatcgag cgcctcggcg
      181 ggacgcgcgc gatccccgtc gatgtgcgcg tcatcacggg caccaagcgg ccgctcaaag
      241 aggtcgttgc ggaagggcgc tttcgcgagg acctgttcta ccggctgaac gtgattccga
      301 tcaacctccc gccgctgcgc gagcgtcgcg aggacgtgcc gattctcctg gaacatttcc
      361 tccgccggtt cttcgccgag cggggcgagg agccgaggcc catcgcgccg gcggtccagc
      421 aggcgttcct gcgttatccg tggcccggca acgtccggga actggagaac gcgtgcgaac
      481 ggatcgccca gacctgcacc tgcgagacgg tgcggctggg gtgcgtccca atcaacgtgc
      541 tcttccaccg ccacgtcgag gagcagtcgc ctgtcgtcga agcgcaccag gagccgacgg
      601 cgatttccct cgaagagcgg ctgcgcgagg tcgaactcaa cctgatcagt tgggccctca
      661 aggccagcgg cgggaacaag tcgcgtgccg ctgaactcct caacgtcaag cgctcgaccc
      721 tcggcgatcg cgtcaagaag ctcgggctcg agaagctcca ggcccgggac tagcggccgc
      781 acgggcgccc ggccggcggc gcgccggctc cggccgggcc gccgggcaaa tcctcttcgc
      841 ctggcgggaa cgccgccgtc gggcgtgcgc cacgcctccg agcaagtcct cgaggcttcc
      901 ggcctggcgt gcgcggccag gaacgagcct cggctggtcc ttcagcctct gccggcgctc
      961 ccggacgggg tccggcccgg tagtggcgat tggtgcgcgg ccatgccggc ctcgcgcgcg
     1021 cggttcgaga agcgcttcgt ccggcgtcga ggcagcggac cgatcacgcc ttgtcagggg
     1081 ctgccagcgc ctccgggacg ccgtagacat cgcagcggaa cccggccagc ctgacgcgct
     1141 gcgcgatctc gaggagccgt gcggccggaa cctgctgcag gtacgggaac gcgggtatcc
     1201 ggtcgatggt gtagacctgg acggccttgg gccggatgcg gtcgagcgcg ccgacccagt
     1261 tgatcaccgt caggtccccc gtgttgtcga tccggccggt ccgatccctg acgaacatcg
     1321 actggatgac gatgtccggc accttcttga ggccggcgat cacctgttcg atggtcatcg
     1381 tggcgccgtt gaccgtccgc agcgtggccg tgtcgcccgc gtcgagcttc atgtagcgct
     1441 catccagcgc ggcgagcgct tcgcggacct cggcttgttc gagggtgctc gcgttcgaca
     1501 gcaccgccag cggtacgccc ggtgcgaggt cgtcgcgcac ggcccgcagc gcccgcacca
     1561 cctcccgaaa cttcggatgc agcgtcggct ccccgtggcc ggcgagcgtg agcctgtcga
     1621 ggcgctcgcc ctgggccgac agcgcgcgga tcgctgaggc gacggccttg gcgacggcgg
     1681 acgggctcgg ccagtgctcg aatgcgggtg tcgccgtgcg gcgcgagtgc gcggaccagc
     1741 cgtactggca gtacgagcag ttgaaggtgc acagcttgag gtgcgccggc gtcaggttga
     1801 tgccaagcga tcggccgagg cggcgcgagc ggaccgggcc gtaaacgatg ccctcgtgaa
     1861 gggccatcag ggtggccgtc gccgcgggcc gaaccacgcg gacccgagcg gccgccttgg
     1921 atgcggccgc cggcggctcc ggtgcgaccg ccgctggagc aggcgaggct ttcgtgctgg
     1981 ccgattccgc ctgggcggcg gtcgccgtgg cggccttggc ggctcggacc ggcggctgcg
     2041 gctttctggg actgcctgcg gtcgagcgct tcactgcgga cttcttcacg gacacctcca
     2101 ccccgccggg tgccgcgcgc cattcgcgcg gccgcacgaa cccgcggagg gaaacccgtc
     2161 gccggctgac gcggggcggg cgaagggcat tcggagcgct gcctggccgg acagcgcctg
     2221 ctccaatcca ttatggcacg atcggcgcac tcggcgccgc cgccgcggac gggcgcttgg
     2281 gccggagatc tcgggaacgc gtcgctcgcg cgcccgcaag cgtgcgggag gctggggcaa
     2341 agccgcggcg cggcgactgg cgtgccgccc ttgtcgtttg ctccgagcca tccagtcgat
     2401 cggcgcctcc cgccggccag gctccgattg tcaggacgcg ctcacggcga gccggcggct
     2461 tcgcggaccg gcggacagga gcgccggatg cagccaggcg cccggcgggg ccgccggcta
     2521 ccgttcaagg ccgaccccgc cggggagcgc caacggagtc ccgccttcag cctcgatccg
     2581 ctggtattcg cggaacgcct gccgcaggtg ctgcgccgcc gcctcgcgct gggcggggct
     2641 cagcaggatc tggcgcgaca agacggcggc catggttccg ctgggagtcg cgaggtagcg
     2701 ctcgagcgtc acaccaaacc gcttctgcgt gtcgtcggcg gccgtggaga ggtccgggcc
     2761 gattggcgtg gggctcgtca tgccgagcac ggccacgctg tggcagaccg cgcagcctgc
     2821 ctgcgcgaac cagccgccgc gcccgctccg cagccgcgtt tcggccagct gctcctccgc
     2881 tgacggcagg tcctcggccg gtgtgccggg cagatacgcg gctccaaagg cgactctccg
     2941 ccaggacatc acgagcaaag ccagcgcctg gcactcccgg acggacaggt ggaagttggg
     3001 catcagggtg tccgcgacaa cgagcctcgg gtccttcagg tgcgcgagat gccaggcgaa
     3061 gacggttgga cggccccgca gccggctgta gtcgaactgc tcagccgact tgtcgccaac
     3121 ccacgtcagg tcgggcccga cggcgccgcc gcgcccgttc acggcgtgac acgagcggca
     3181 tcccttctgc cgcaccaggc tctttccgag attgatcgca ccggccccct cggtggcgcg
     3241 ctcgtagcgg tggcacaggt tgcagttcga ctggaggagc ggcggagctt ctcccgatgt
     3301 cgagtagagc gcggccaact cgcggtccag caacggttcg ccccagtgcg ggacatcgcc
     3361 gtgcgcgacg ggcagggtca ccgcccac
//
LOCUS       DAOSIF010000343         3386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24459_length_3386_cov_3.593816, whole genome shotgun sequence.
ACCESSION   DAOSIF010000343 DAOSIF010000000
VERSION     DAOSIF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24459_length_3386_cov_3.593816"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="PLT35_10750"
     CDS             complement(<1..1704)
                     /locus_tag="PLT35_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG29744.1"
                     /translation="MTNRMLKNLPSALCLVLLAAASASAQPGEVFISEYIEGSSNNKA
                     IEIYNGTGRAIDLAAGGYTIKMFFNGSATAGATITLTGTVAPGDVHVVAHASANAAIL
                     AQADQTTGASWYNGNDAVVLLKGTAVIDAIGQVGFDPGTEWGTGLTSTADNTLRRKAS
                     VCAGDTNPADPFDPAIEWDGYAIDTFAGLGAHTANCGPASAPVLAVCGAPLSLVQGTA
                     GSRDVTASDADGTVVSLELTSVTPAPAAGAISRTAFTPALAVGGTATSTISVSADVPA
                     GSYEALISAANSDPTPQTATCTLSVRVIQPVEIWEIQGAGLASPLEGQTVFTADNIVT
                     ALGYYQGAPNGFFMQTPDARADSSASTSNGIFVYTGGVPAVQVGDQVDVLGTVAEYFG
                     LTELTTPTVTVDSSGHALPAAVLLGEIEPGVFVPSHSQPWPAIELERFEGMRVRVENG
                     RAAAATDQYGDIAVVAASSRPFREPGIAYPGDPLHPIVWDGNPEIFEINPDGAGLANV
                     SVPAGSVIEVAEGPLTYAFSSYQIWPTTFAYTAPALPRPVRARRAGEMTVATQNLLHF
                     FD"
     gene            1976..>3386
                     /locus_tag="PLT35_10755"
     CDS             1976..>3386
                     /locus_tag="PLT35_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOG29745.1"
                     /translation="MRTAALLLALAASAAPALAADRPNILFIMSDDHAAHAISAYGSR
                     VNVTPNIDRLAREGMRLDNVFVTNSICTPSRAAILTGQYSHVNGVPVFNRFDSSRQTV
                     AKLLQAGGYHTGMIGKWHLGSDPAGFDEWEILPGQGDYFNPVLYTATGEKRYDGRYVT
                     DVLTDLAIGFLEKRPAGKPFFLMWHHKAPHRNWEPDEAHRRQFENRWIPEPPTFWDTY
                     ATRTDALRENRQRVADDMTNNDLKRRPPAGLTGPALVKWLSERPAEVTIERSGAPLTL
                     RGEALARWKYQRYMQDYLATIQSVDDGVGRVLDHLDRAGLARNTIVIYTSDQGFFLGD
                     HGMYDKRFMYEESLRMPFLVRWPAGIEAGGTTRAMALNVDFAPTFLEAAGLPVPPDMQ
                     GRSLLPVLRGQTPADWRTSMYYRYYHDPGHHNTAAHYGVRTLTHKLIHYWKRDQWELF
                     DLVNDPMEMSNLYGQPGHER"
BASE COUNT          511 a         1208 c         1186 g          481 t
ORIGIN      
        1 gtcgaagaag tggagcaggt tctgggtcgc caccgtcatc tcgcccgccc ggcgcgcgcg
       61 cacggggcgc ggcagcgccg gcgccgtgta cgcgaacgtc gtcggccaga tctggtagct
      121 ggagaaggcg tacgtgagcg gcccttccgc gacctcgatg accgacccgg ccggcaccga
      181 cacgttcgcc aggccggccc cgtccgggtt gatctcgaag atctccgggt tgccgtccca
      241 gacgatcgga tgcagggggt cgcccgggta tgcgatgccg ggctcacgga acggccgcga
      301 gctcgccgcg acgacggcga tgtccccgta ctggtccgtg gcggcggccg cgcggccgtt
      361 ctcgacccgc acgcgcatgc cctcgaaccg ctccagctcg atggccggcc acggctgact
      421 gtgcgagggc acgaacacgc ccggctcgat ctccccgagg agcacggccg ccggcagcgc
      481 gtggccggac gaatcgacgg tgaccgtcgg ggtcgtcagc tccgtcaagc cgaagtactc
      541 ggcaaccgtt cccagcacgt ccacctggtc gcccacctgc acggccggca cgccgcccgt
      601 atagacgaag atgccgttcg acgtgctcgc cgacgagtcc gcgcgcgcgt cgggcgtctg
      661 catgaagaag ccgttggggg cgccctggta atagccgagc gcggtgacga tgttgtccgc
      721 ggtgaagacg gtctggccct cgagcggcga cgcgaggccg gcgccctgga tctcccagat
      781 ctcgaccggc tggatgaccc gcacgctcag cgtgcacgtg gccgtctgcg gcgtcgggtc
      841 gctgttggcg gcgcttatca gcgcctcgta gctgcccgcc ggcacgtccg cgctgaccga
      901 gatcgtcgag gtcgccgtgc cgccgaccgc cagcgccggc gtgaacgccg tgcggctgat
      961 cgcgccggcg gccggcgcgg gcgtcacgct cgtcagctcg aggctgacca ccgtgccgtc
     1021 ggcgtcgctc gcggtcacgt cgcgcgagcc cgcggtgccc tggacgaggc tgagcggggc
     1081 gccgcagacc gccagaacgg gcgcgctcgc ggggccgcaa ttcgccgtgt gcgcgccgag
     1141 gccggcgaag gtgtcgatgg cgtacccgtc ccactcgatc gccggatcga acgggtcggc
     1201 ggggttcgtg tcgccggcgc agaccgacgc cttgcggcgg agcgtgttgt ccgccgtgga
     1261 cgtcaggccg gtgccccatt cggtccccgg gtcgaagccg acctggccga tggcgtcgat
     1321 gacggccgtg cccttcagca gcacgaccgc gtcgttgccg ttgtaccagc tcgcgccagt
     1381 cgtctggtca gcctgggcga gaatcgcggc gttggccgat gcgtgtgcca cgacgtgcac
     1441 gtcgcccggc gccaccgtgc cagtgagggt gatcgtggcc ccggccgtgg cgctcccatt
     1501 gaagaacatc ttgatggtgt agcccccggc ggccagatcg atggccctcc cggtgccgtt
     1561 gtagatctcg atggccttgt tgttcgagct gccctcgatg tattcggaga tgaacacctc
     1621 gcccggctgg gcggacgcgg acgcggcggc cagcaggacc aggcacaaag cggacggcag
     1681 gttcttcagc attctgttgg tcatggtgtc ctcgggcggg cacgaacggc gatctggcag
     1741 gctccggaag gccagggagg cattctacac tggtccgggc cccggccgga cgaagtcccg
     1801 gcgcctccct tcggctcgcc gggcagcgcg ttgtcgatgt ccggcgccga cgcgggccct
     1861 cgggagagcc tcggcaaggg cctgcaggcg gcccccggcg gcagggccgg cgacggcgcc
     1921 gtcatcggat ggccgggcgc acgagatccc gcccggtccg cgatagaatc gccccatgcg
     1981 caccgccgcc ctcctgctcg cgctggccgc gtccgcggcg ccagccctgg ccgccgaccg
     2041 gccgaacatc ctcttcatca tgtcggacga tcacgccgcc cacgccataa gcgcgtacgg
     2101 ctcccgggtg aacgtcaccc cgaacatcga tcgcctcgcc cgcgagggca tgcgcctcga
     2161 caacgtcttc gtgaccaact cgatctgcac gccgagccgg gcggccatcc tcacgggcca
     2221 gtactcgcac gtgaacggcg tgccggtgtt caaccggttc gacagttcgc gccagacggt
     2281 ggcgaagctc ctccaggcgg gcggctacca cacgggcatg atcggcaagt ggcacctagg
     2341 cagcgacccg gcggggttcg atgagtggga gatcctcccg ggccagggcg actacttcaa
     2401 tcccgtgctc tacaccgcga cgggcgagaa gaggtacgac ggccgctacg tcaccgacgt
     2461 cctcacggat ctggccatcg gcttcctcga gaagcgcccg gccgggaagc cgttcttcct
     2521 gatgtggcac cacaaggccc cgcaccggaa ctgggagccc gacgaggcgc accgcaggca
     2581 gttcgagaac cgctggatcc ccgagccccc gaccttctgg gacacctacg ccacgcggac
     2641 cgacgccctg cgcgagaacc ggcagcgcgt ggccgacgac atgacgaaca acgatctgaa
     2701 gcgcaggccc ccggccgggc tgacggggcc cgcgctcgtg aaatggctgt cggagaggcc
     2761 ggcggaggtc accatcgagc gcagcggcgc gccgctcacg ctgcgcggcg aggcgctcgc
     2821 ccgctggaag taccagcgct acatgcagga ctacctcgcc acgatccagt ccgtggacga
     2881 cggcgtcggg cgggtgctgg atcacctcga ccgggcgggg ctcgcgcgga acacgatcgt
     2941 catctacacg agcgaccagg ggttcttcct gggcgatcac gggatgtacg acaagcgctt
     3001 catgtacgaa gagagcctgc ggatgccctt cctcgtccgg tggcccgcgg gcatcgaggc
     3061 gggcggcacg acgcgcgcga tggcgctcaa cgtggacttc gcgccgacgt tcctcgaggc
     3121 ggccggcctg ccggtcccgc ccgacatgca gggccgcagc ctgctcccgg tgctgcgcgg
     3181 gcagacgcct gccgactggc gcacgtcgat gtactaccgc tactaccacg acccgggcca
     3241 ccacaacacc gcggcgcact acggggtccg gacgctcacg cacaagctga tccactactg
     3301 gaagagggac cagtgggagc tgttcgacct cgtgaacgat cccatggaga tgagcaacct
     3361 ctacggccag ccgggccacg agcggg
//
LOCUS       DAOSIF010000344         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24475_length_3384_cov_4.430460, whole genome shotgun sequence.
ACCESSION   DAOSIF010000344 DAOSIF010000000
VERSION     DAOSIF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24475_length_3384_cov_4.430460"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLT35_10760"
     CDS             <1..190
                     /locus_tag="PLT35_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29746.1"
                     /translation="NSVEAVIASLRNTLEFVREQDARDREEKILLHRPRPLDSREVMP
                     GWSQDRKDDGPVVVERHA"
     gene            246..1502
                     /gene="hutI"
                     /locus_tag="PLT35_10765"
     CDS             246..1502
                     /gene="hutI"
                     /locus_tag="PLT35_10765"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOG29747.1"
                     /translation="MPRRSIIRGARVVFTCAGGAPKSGPAQADARPISGASVVATDGV
                     IDYVGHAGDADLLAARPGDTVIDAAGGTVVPGLVDPHTHAVFGGDRREELRRRLGGAT
                     YAEIAAGGGGILSTVRATREASEEALVASAKARLDQMLACGTTTCEIKSGYGLQPDAE
                     LKILRAIRRLKDEHPIDVVATFMGAHEIPAEHRANRRRYIDQVVGEMIPAAARAGLAE
                     WCDVFCESGVYTPEEAAEILEAGARHGLKPRIHADELAESGGALVAARVGATSADHLL
                     HVTPRGIHAMKEAGVVATLLPIAAFYLKLGRFAPARDLIEGGVPVALASDLNPGGGFS
                     PSMPFAMTLACFAMRMTVEEALVAATINAAYAIDRHDRAGSLEAGKDLDAVVVDGDAV
                     DLLKVGAPAIRAVVKRGQVVWRRPVT"
     gene            complement(1495..1854)
                     /locus_tag="PLT35_10770"
     CDS             complement(1495..1854)
                     /locus_tag="PLT35_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29748.1"
                     /translation="MGRLLRLAVAFAVAWAAWHAGWAAWNQFRFSSDVDEIAKFGPEQ
                     DERAVRGAVLEAAAKYGLPVEERAVRIRRQDAPAHLYIDVDYTVPIEVLPRVTYPWTF
                     ATHAHGWFVPGGRAPVK"
     gene            complement(1857..2507)
                     /gene="lepB"
                     /locus_tag="PLT35_10775"
     CDS             complement(1857..2507)
                     /gene="lepB"
                     /locus_tag="PLT35_10775"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG29749.1"
                     /translation="MVPFKKSVVREYLETILVAVILALFFRTFVVQAFKIPTGSMEPN
                     LLVGDQLLVNKFVFAPAHSSAERAILPIRSVRRGDVVVFKFPVNPERDFIKRVVGLPG
                     ETVELKDTRVYVNGRPLDEPYAHYLVSRPGPAGAGDGGPEDVRNHYGPVPVPDGALFV
                     MGDNRDNSEDSRYWGFMPIQNLKGRALMIYWSWDGSGGTLNPLRQTRWARLLRQIH"
     gene            complement(2507..>3384)
                     /locus_tag="PLT35_10780"
     CDS             complement(2507..>3384)
                     /locus_tag="PLT35_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOG29750.1"
                     /translation="ELRDALEKLRLNDASFFYEPESSTALGFGFRCGFLGLLHMDIVQ
                     ERLEREFDQDLVTTAPGVLYRVTTTDGEVHEIDSPAKLPDTGRIAKFEEPVIRAMILT
                     PAEHVGAVLQLCQDKRGVQKALEYLASDRVLVTYDLPFAEVVLDFYDRLKTLSRGYAS
                     LDYHVSGYWESPLQRLEILVNGDPVDALSVIVHRDSAYARGRALASKMRALIPRQLFE
                     VAIQACIGNRVIARESVKPMRKNVLAKCYGGDITRKRKLLEKQKAGKKRMKRVGKVDI
                     PQEAFLAVLKIGLEE"
BASE COUNT          504 a         1188 c         1200 g          492 t
ORIGIN      
        1 gaactcggtg gaggccgtca tcgcctcgct gaggaacacg ctcgagttcg tgcgcgagca
       61 ggacgcgcgg gaccgcgagg agaagatcct gctgcaccgg ccgcggccgc tggactcgcg
      121 cgaggtcatg cccggctgga gccaggatcg caaggacgac ggccccgtcg tcgtcgagcg
      181 gcacgcctga ctggcggccg cggcgcgccc cggccgccgg gggcgcggga ggatgccctg
      241 ccgtcatgcc ccgacgatcg atcatccgcg gcgcccgtgt cgtgttcacg tgcgccggag
      301 gcgcgccgaa gtcggggccg gcccaggctg acgcgaggcc gatctccggc gcctccgtcg
      361 tcgccacgga cggcgtgatc gactacgtcg gacacgccgg cgacgccgac ctcctcgcgg
      421 cccgccccgg cgacaccgtc atcgacgctg ccggcggcac cgtcgtgccc gggctcgtcg
      481 atccgcacac gcacgccgtc ttcggcggcg accggcgcga ggaactccgg cgccggctgg
      541 gcggggcgac ctacgccgag atcgccgccg ggggcggcgg catcctgtcc acggtgcgcg
      601 ccacgcggga ggcctccgag gaggcgctgg tcgcctcggc gaaggcccgg ctggaccaga
      661 tgctcgcgtg cgggacgacg acgtgcgaga tcaagagcgg ctacggcctt cagccggacg
      721 ccgaactgaa gatcctccgc gccatccggc gcctgaagga cgagcacccg atcgacgtcg
      781 tggcgacctt catgggcgcg cacgagatcc cggcggaaca ccgcgcgaac cggcggcgct
      841 acatcgatca ggtcgtcggc gagatgatcc ccgccgccgc ccgggccggc ctcgccgagt
      901 ggtgcgacgt cttctgcgag agcggggtct acaccccaga ggaagccgcg gagatcctcg
      961 aggccggcgc gcgccacggc ttgaagcccc gcatccacgc cgacgagctg gccgagagcg
     1021 gcggcgcgct cgtcgccgcg cgcgtgggag cgacgtcggc ggaccatctc ctccacgtga
     1081 cgcccagggg catccacgcc atgaaggagg cgggcgtcgt cgccacgctg ctgccgattg
     1141 ccgccttcta cctgaagctg ggccgcttcg ccccagcccg cgatctgatc gagggcggcg
     1201 tgcccgtggc gctcgcgagc gacctcaacc cgggcggcgg cttctcgccg tcgatgccct
     1261 tcgcgatgac gctggcgtgc ttcgcgatgc ggatgacggt ggaagaggcg ctcgtggcgg
     1321 ccacgatcaa cgcggcctat gccatcgacc gccacgaccg cgcgggcagc ctcgaggcgg
     1381 gcaaggatct cgatgccgtc gtcgtggacg gcgacgcggt cgatctcctg aaggtcgggg
     1441 cgccggcgat ccgggccgtc gtcaagcgcg gacaggtggt ctggcgccgg ccggttactt
     1501 gactggcgcg cggccgcccg gcacgaacca gccgtgggcg tgcgtcgcga acgtccaggg
     1561 gtaggtaacc cgaggcagga cctcgatggg caccgtgtag tccacgtcga tgtagaggtg
     1621 cgcgggagcg tcctggcggc ggatgcggac cgcccgttcc tcgacgggca ggccgtactt
     1681 ggccgcggcc tccagcacgg cgccccgcac cgcgcgctcg tcctgctcgg ggccgaactt
     1741 cgcgatctcg tcgacgtcgc tggagaaccg gaactggttc caggcggccc acccggcgtg
     1801 ccaggcggcc caggcgacgg cgaaggcgac ggcgagcctc aggaggcgtc ccatactcag
     1861 tggatctgac gcaagagccg cgcccacctg gtttgcctca gcggattgag cgtcccgccc
     1921 gacccgtccc aggaccagta gatcatcagg gctcggccct tcaggttctg tatcggcatg
     1981 aagccccagt agcgtgagtc ctccgagttg tcgcggttgt cgcccatgac gaagagcgcg
     2041 ccgtcgggga ccggaacggg cccgtagtga ttcctgacgt cctcggggcc gccgtcgccc
     2101 gcgcccgccg gccccggacg gctgaccagg tagtgggcgt acggctcgtc gagggggcgg
     2161 ccgttcacgt agacgcgcgt gtccttcagt tcgacggtct cgcccgggag cccgacgacg
     2221 cgcttgatga agtcgcgctc ggggttcacc gggaacttga agaccacgac atctcccctc
     2281 cggacggacc tgatcggcag gatcgcccgc tcggcgctcg agtgagccgg cgcgaagacg
     2341 aacttgttca cgagcagctg atcgccgacg aggaggttcg gctccatcga tccggtgggg
     2401 atcttgaacg cctggacgac gaaggtccgg aagaacagcg ccaggatcac cgcgacgagg
     2461 atcgtctcga ggtactcgcg gacgacggac ttcttgaacg gcaccactac tcctccagcc
     2521 cgatcttgag gacggccagg aacgcctcct gcggaatgtc caccttgccc acgcgcttca
     2581 tgcgcttctt gccggccttc tgcttttcca ggagctttcg cttccgcgtg atgtcgccgc
     2641 cgtagcactt ggccagcacg ttctttcgca tgggcttcac cgactcgcgc gcgatgacgc
     2701 ggttgccgat gcaggcctgg atggccacct cgaagagctg ccgcgggatg agcgcgcgca
     2761 tcttcgaggc cagcgcgcgg ccgcgcgcgt aggccgagtc gcggtgcacg atgaccgaca
     2821 gcgcgtccac cgggtcgccg ttgaccagga tctcgagccg ctgcagcggc gactcccagt
     2881 agccgctgac gtggtagtcg agcgacgcgt acccgcggga gagggtcttc aggcggtcgt
     2941 agaaatccag cacgacctcc gcaaacggca ggtcgtaggt gaccaggacc cggtcgctcg
     3001 cgaggtattc gagcgccttc tgcacgccgc gcttgtcctg gcacagctgc aggacggcgc
     3061 ccacgtgctc ggcgggcgtg aggatcatcg cccggatgac cggctcctcg aacttcgcga
     3121 tccggccggt gtcgggcagc ttcgcggggc tgtcgatctc gtggacctcc ccgtccgtgg
     3181 tcgtcacccg gtacagcacg cccggcgccg tcgtcaccag gtcctggtcg aactcgcgct
     3241 cgaggcgttc ctgcacgatg tccatgtgca ggaggcccag aaacccgcag cggaacccga
     3301 agcccagggc cgtcgacgac tcgggctcgt agaagaacga ggcgtcgttc agccggagct
     3361 tctcgagggc gtcgcgcagt tccg
//
LOCUS       DAOSIF010000345         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24477_length_3384_cov_3.823671, whole genome shotgun sequence.
ACCESSION   DAOSIF010000345 DAOSIF010000000
VERSION     DAOSIF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24477_length_3384_cov_3.823671"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLT35_10785"
     CDS             <1..279
                     /locus_tag="PLT35_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29751.1"
                     /translation="PVTVQLRVRPFSPPGASPALAVRVNGRAYGPFPIVPGWQRVDFP
                     TDAEAWRAGVNRVQLVWPAAAVPARVGVGGDTRELGGSVDYLRIALPR"
     gene            complement(299..826)
                     /locus_tag="PLT35_10790"
     CDS             complement(299..826)
                     /locus_tag="PLT35_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG29752.1"
                     /translation="MREQRGFTLIELLIVVAIIGVIAAVAVPGLVRSRQTANETSAIA
                     SLRAITEAQSLYSASCGNGFYSPSLSNLGTPPPGGTAFISPDLSTGNSVFKSGYQVTM
                     GSSSGPAADAPPTCNNLAAGAAVQGYFATATPSPGGGTKAFGTNLNHTIYYATQAAPL
                     AMTDMTAPAGALSIQ"
     gene            complement(924..2474)
                     /locus_tag="PLT35_10795"
     CDS             complement(924..2474)
                     /locus_tag="PLT35_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG29753.1"
                     /translation="MRHLFATKSLDQIARDSESAGHALKRTLGPVQLTALGIGAIMGA
                     GIFSTIGSAAAGGGTHTGAGPAIIASFVLTAVACGFAALCYAEFAAMVPISGSAYTYA
                     YATLGELVAWIIGWDLILEYAVGNVAVAISWSGYFRELLAGFGVYIPGWLCIDYHTAA
                     RAAEQVAALQAQGGDAAGLGIVVTRSAAAFAAAPHLFGIPIVFNLPAFVIVMAITWVL
                     VVGIRESAWVTSVLTVMNVAILVFFCVVGAFYVRPANWVPFAPNGFAGISSAAAIIFF
                     AYIGFDAVSTAAEETRNPQRDMPIGMIASLVICTVIYIAVAMVLTGMLPWRQLGTAEP
                     LATAFSANGITWASGIIAFGAVCATSGVLLVFQLGQPRIFFSMARDGLLPKWAARVHP
                     EYRTPHVTTILTGVLVAVFATFLNINEVVELTNIGTLFAFVLVSAGILILRRTDPSRV
                     RPFRTPFVPWVPLIAIAMCLYLMFHLPLLTWIRFFVWLAAGAAIYLVYGVRHSVLRSR
                     AGNRAGLP"
     gene            complement(2471..>3384)
                     /locus_tag="PLT35_10800"
     CDS             complement(2471..>3384)
                     /locus_tag="PLT35_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29754.1"
                     /translation="MPEVRVGDTPARVLMASESRLSVEVPAGLARGRYEVQVDGVEGA
                     ASLDVGAPLVTGVHQVDSPAFGPDGTLYVTFSGTRGQRVPVSVFRVGPDGEKAPFLTG
                     IANATSMAFDAGGALHVTSRLDGCVYRADETGRLEVVARELGTACGIAFGSDGTMFVG
                     DRSGTVFRIGSASRVVPFASLPPSLAAFHLAAGPADELFVTAPTMATRDGVYRIDRQG
                     VVVKVADGFGRPQGMAVDAEGALYVVDAVAGGAGLYRLRSGARRELVLAAPALVGAAF
                     DPRGGLVVASNDTVYRLDVALRPWSFA"
BASE COUNT          544 a         1200 c         1209 g          431 t
ORIGIN      
        1 ccggtcaccg tgcagctccg cgtgcggccg ttctcgccgc ccggcgcgtc gccagccctg
       61 gccgtgcgcg tcaatggtcg cgcctacggc ccgttcccga tcgtgccggg ctggcagcgc
      121 gtggacttcc cgaccgatgc ggaggcgtgg cgggcgggcg tgaatcgcgt gcagcttgtc
      181 tggcccgccg ccgccgtccc cgcgcgggtc ggcgtcggcg gcgacacgcg cgagctcggc
      241 ggctcggtgg actacctgcg gatcgcgctc ccgcgctgac gcgcgaggcg gcgccaggct
      301 actggatgga gagcgcgccc gccggggccg tcatgtcggt catcgcgagc ggcgcggcct
      361 gcgtggcgta atagatggtg tggttgaggt tcgtgccgaa ggccttcgtg ccgcctcccg
      421 gcgacggcgt cgccgtggcg aagtagccct gcacggcagc cccggccgcc agattgttgc
      481 aggtcggcgg cgcatccgcc gcaggcccgc tcgagctgcc catcgtcacc tggtatccgc
      541 tcttgaagac gctgttcccg gtgctcagat cggggctgat gaacgccgtg ccgcccggcg
      601 gcggagtgcc gaggttcgag agcgacggcg agtagaaccc gttcccgcag ctcgccgagt
      661 acagggactg cgcctcggtg atggcgcgca gcgaggcgat ggcggacgtc tcgttggccg
      721 tctgccgcga gcggaccagc cccggcaccg ccacggccgc gatgacgccg atgatcgcga
      781 cgacgatcag cagttcgatg agcgtgaacc cgcgttgctc gcgcatgatg ccaccccggc
      841 cgcccgtctc agccccagcc ggcgcccgcc gtgacgcgtg acgggccgcc cgtcttcaac
      901 gatgcccctc tgccgaggga atgtcaaggc agcccggccc ggttccctgc gcgcgacctg
      961 agcacgctgt ggcgcacgcc gtacaccagg tagatcgcgg ctcccgccgc cagccacacg
     1021 aagaagcgga tccacgtgag cagcggcaag tggaacatca ggtacaggca catggcgatc
     1081 gcgatgagcg ggacccacgg cacaaacggc gtgcggaacg ggcgcacccg gctcgggtcg
     1141 gtccggcgca ggatcaggat gccggccgag acgaggacga aggcgaacag cgtcccgatg
     1201 ttcgtcagct ccacgacctc gttgatgttc aagaaggtcg cgaagaccgc gacgaggacc
     1261 cccgtcagga tcgtggtgac gtgcggcgta cggtactccg ggtgcacgcg ggcggcccac
     1321 ttcggcagca ggccgtcgcg cgccatcgag aagaagatgc gaggctggcc gagctggaac
     1381 accagcagca cgccgctcgt ggcgcagacc gctccgaacg cgatgatgcc cgacgcccag
     1441 gtgatgccgt tggccgagaa ggcggtcgcc aggggctcgg cggtcccgag ctggcgccac
     1501 ggcagcatgc ccgtcaggac catcgccacc gcgatgtaga tgacggtgca gatcaccagg
     1561 ctggcgatca tgccgatggg catgtcgcgc tgggggttcc tcgtctcctc ggcggcggtg
     1621 gacaccgcgt cgaagccgat gtaggcaaag aagatgatcg cggcggcgct cgagataccc
     1681 gcgaacccgt tgggggcgaa cggcacccag ttggccggcc ggacgtagaa ggcgccgacc
     1741 acgcagaaga aaaccaggat cgcaacgttc atcaccgtga gcacgctggt cacccacgcg
     1801 ctttcgcgga tgccgacgac cagcacccag gtgatggcca tcacgatcac gaacgccggc
     1861 aggttgaaga cgatcgggat gccgaagagg tgcggcgcgg cggcgaaggc ggcggcgctg
     1921 cgtgtcacga cgatgcccag gccggccgcg tcgccgccct gggcctggag ggccgccacc
     1981 tgctcggcgg cccgcgcggc ggtgtggtag tcgatgcaca gccagccggg aatgtagacg
     2041 ccgaagccgg cgagcagttc gcggaagtag cccgaccacg agatcgcgac ggcaacgttg
     2101 ccgacggcgt actccaggat gagatcccag ccgatgatcc acgccaccag ctcgcccagc
     2161 gtcgcgtagg cgtaggtgta ggccgagccc gaaatgggca ccatggcggc gaactcggcg
     2221 tagcagagcg ccgcgaagcc gcacgccacg gcggtcagca cgaaggacgc gatgatggcg
     2281 ggccccgccc cggtgtgcgt cccgccgcca gccgccgccg acccgatcgt cgagaagatc
     2341 cccgccccca tgatggcgcc gatccccagg gcggtcagct gcaccggacc cagcgtgcgc
     2401 ttcagcgcgt ggccggccga ctcgctgtcc cgcgcgatct ggtcgagcga cttcgtggcg
     2461 aacaggtgtc tcatgcgaag ctccaggggc gcagggcgac atcgaggcgg tagacggtgt
     2521 cgttggacgc gaccaccagc cccccgcggg gatcgaatgc ggcgcccacg agcgcggggg
     2581 ccgccagaac gagttcgcgg cgcgcgccgg accgcaggcg gtagaggccc gcgccgccgg
     2641 ccacggcgtc caccacgtag agcgcgccct cggcgtccac ggccattccc tgcgggcggc
     2701 cgaatccgtc cgcgactttg acgaccacgc cctgccggtc gatccggtac acgccatcgc
     2761 gggtcgccat cgtcggggcg gtcacgaaga gttcgtccgc cgggccagcc gccaggtgga
     2821 aagccgcgag actcggcgga agcgacgcga agggcacgac ccgggaggcc gagccgatgc
     2881 ggaacaccgt tccgctccgg tcgcccacga acatcgtgcc gtccgagccg aaggcgatgc
     2941 cgcaggcggt ccccagctcg cgcgccacca cctcgagccg gcccgtctcg tccgcgcggt
     3001 agacgcagcc atcgagacgg ctcgtcacat gcagcgcgcc tccggcgtcg aaggccatgg
     3061 acgtggcgtt cgcgatgccg gtcaggaatg gcgccttctc gccgtcgggc ccgactcgga
     3121 acacggatac gggcacgcgc tgaccgcggg tgccgctgaa cgtcacgtag agcgtcccgt
     3181 cgggcccaaa agcggggctg tcaacctggt gcacgcccgt gacgagcggc gccccgacat
     3241 cgagcgacgc cgcgccttca acgccgtcga cctgaacctc gtagcgcccg cgcgccagtc
     3301 cggccggcac ttcgaccgac agccgcgatt ccgaggccat caggacccgc gccggcgtgt
     3361 ctcccacgcg gacctcgggc atgc
//
LOCUS       DAOSIF010000346         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24510_length_3381_cov_3.241130, whole genome shotgun sequence.
ACCESSION   DAOSIF010000346 DAOSIF010000000
VERSION     DAOSIF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24510_length_3381_cov_3.241130"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="PLT35_10805"
     CDS             complement(<1..1744)
                     /locus_tag="PLT35_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG29755.1"
                     /translation="MEGARVRERRARAAAAIAAVAASAALAVSAGAQGARQAPAGQQP
                     AAPTAQGGSQGQPGDQAQKPPVFRAGVNVVRVDVIVSGKDGEPVKDLTAADFEISEDG
                     KPQAIDLFKLVNSDGNVAPGGAPARPIRSLADQEGEAARDDVRLFVIFLDDYHVRKGA
                     AMRVRETLAEFVRTQLGPLDMVAVMYPLMSVSELAFTRDPGALAASMLAFEGRKFDYR
                     PRNAIEERYAHYPAQAVETIRNDVVMTALRGLAVKLGTLREGRKAVIFVSEGFTALLP
                     PQMRDMNAQLPGLGNPNARNPFAGGSAAEERARSSADGQLLADLRQVFDAANRNNTAI
                     YTLDPRGLAVSEFDINESVGLQTDAEYLRKSQDTLRILASNTDGRAIVNRNDLEGGLR
                     QVVRDSSAYYLIGYTSPLAAPDGKFHEIKVRLRRPGLQVRARKGYWAPTEAEAENASA
                     PPKPPAAPGVAKALSAVENRQRDAYVSTWVGTSRDADGGTRVTVVWEPVPPVPGEKRE
                     EAATVALLASSRGGDEYYRGPVPAETGADAAAAAPPPAAAPARRAASKVSFVAPPGRI
                     QLRLTVQNGRGQVLD"
     gene            1813..2982
                     /locus_tag="PLT35_10810"
     CDS             1813..2982
                     /locus_tag="PLT35_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG29756.1"
                     /translation="MEPNDAPSALTVLSEDERLFRDSVARFADARIRPLVREMDERAA
                     IPQDLVEGLFDLGVMGIEVPEALGGAGARFFHAILAVEEISAVDPAVAVLVDIHNTLV
                     VKALLRWGSAAQRERYLPALATHTVGAYALSEAGAGSDAFSLAARAVADGEGYVLNGR
                     KLWISNAGEAGLFIVFANADPQAGHRGITAFLVDRGTPGFTVGRKEDKVGVRASSTCE
                     LLLEDCRLPASQVLGRPGQGYTIAIELLNEGRIGIGAQMTGLARGAYRHAIQYARERR
                     QFGRPIAEFQLVRAQLARMAADIEAARLLVYNAARLCEAGRPFVTESAISKLFAAEAA
                     ERVTSAAINVYGGCGFVRDFPVEKYWRDAKIGQIYEGTTNIQLLTIAKQLLGERR"
     gene            3108..3317
                     /locus_tag="PLT35_10815"
     CDS             3108..3317
                     /locus_tag="PLT35_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOG29757.1"
                     /translation="MTTITLQVKGMTCGGCESAVKRALARLPGVGAVDASHAEQRVTV
                     TFEAQAVSPDAIRAKISALGYTVLG"
BASE COUNT          449 a         1194 c         1226 g          512 t
ORIGIN      
        1 tgtccaggac ctggcccctg ccgttctgca cggtgagcct cagctggatc cgacccggcg
       61 gggccacgaa gctgaccttc gacgcggcgc gacgcgccgg cgcggcggcc ggcggcggcg
      121 cagcagcggc cgcatcggcg cccgtctccg cgggcaccgg gccgcggtag tactcgtctc
      181 cgccgcggct ggacgcgagc agcgccaccg tggccgcctc ctcgcgcttc tcgccgggaa
      241 ccggcgggac gggttcccac accacggtga cgcgcgtgcc gccgtcggcg tcgcgggacg
      301 tgcccaccca ggtgctgacg taggcgtccc gctgacggtt ctcgaccgcg ctcagggcct
      361 tcgccacgcc gggagccgcc ggcggcttcg gcggcgccga ggcgttctcg gcttccgctt
      421 cagtaggggc ccagtagccc ttccgcgccc gcacctggag gccgggccgc cggagccgga
      481 ccttgatctc gtggaacttc ccatcgggcg ccgccagcgg cgaggtatag ccgatcaggt
      541 agtacgcact gctgtcgcgg acgacctgcc ggagcccgcc ctcgaggtcg ttgcggttga
      601 cgatcgcgcg accgtcggtg ttggacgcca gaatcctgag cgtgtcctgt gacttccgca
      661 agtactcggc gtctgtctgc agccccacgc tctcgttgat gtcgaactcg ctgaccgcga
      721 gcccccgcgg gtcgagcgtg tagattgccg tgttgttccg gttcgcggcg tcgaacacct
      781 gccgcagatc cgccagcagc tgcccgtccg cgctggaacg cgcgcgctcc tccgccgcgc
      841 ttccgcccgc gaaggggttc ctggcattgg ggttgcccag cccgggcagc tgggcgttca
      901 tgtcccgcat ctgcggcggc aggagcgccg tgaagccctc gctgacgaag atcacggcct
      961 tgcggccctc gcgcagcgtt ccgagcttga cggcaaggcc gcgcagcgcc gtcatgacga
     1021 cgtcgttcct gatggtttcg accgcctgcg ccgggtagtg ggcgtagcgc tcctcgatcg
     1081 cgttgcgggg ccggtagtcg aacttgcggc cttcaaacgc cagcatcgac gcggccagcg
     1141 cgccgggatc ccgcgtaaag gccagctcgg acaccgacat cagcgggtac atgaccgcga
     1201 ccatgtccag cggcccgagc tgcgtccgca cgaactccgc cagcgtctcg cgcacgcgca
     1261 tcgcggcgcc tttgcggacg tggtagtcgt cgaggaagat cacgaacagc cgcacgtcgt
     1321 cccgggcggc ctcgccctcc tggtcggcga gcgaccggat cgggcgcgcc ggcgcgccgc
     1381 cgggcgccac gttcccgtcc gagttgacga gcttgaagag gtcgatcgcc tgcggcttgc
     1441 cgtcctccga gatctcgaag tctgcggccg tcaggtcctt gaccggctcg ccgtccttcc
     1501 cggacacgat gacgtccacg cgcacgacgt tgacgccggc ccggaagacg ggcggcttct
     1561 gggcctggtc ccctggctgg ccctgcgacc cgccttgcgc cgtgggcgcc gcgggctgct
     1621 ggcccgccgg cgcctgcctg gccccctggg cccccgccga cacggccagg gccgcgctcg
     1681 ccgccacggc ggcgatggcc gccgcggccc gcgcccgccg ctcccgcacg cgcgcgccct
     1741 ccatggttcg atgataacat cgggctcgat cccgcgatcg aaccgcaggc ctgctctgat
     1801 caggagcccg acgtggagcc caacgacgcg ccctcggcct tgaccgtgct gtccgaggac
     1861 gagcgcctgt tccgcgacag cgtggcccgc ttcgccgacg cgcggatccg gcccctggtc
     1921 cgggagatgg acgagcgcgc ggcgattccg caggacctcg tcgaggggct gttcgatctg
     1981 ggcgtgatgg gcatcgaggt tcccgaggct ctcggcggcg ccggcgcgag gttcttccac
     2041 gccatcctgg ccgtcgagga gatctccgcg gtggacccgg cggttgcggt gctggtggac
     2101 atccacaaca cgctcgtcgt caaggcgctc ctgcggtggg gatcggccgc gcagcgcgag
     2161 cgctatctcc ccgccctggc gacgcacacc gtcggagcgt acgcgctgtc cgaggccggg
     2221 gccggcagcg acgcgttctc gctcgcggcc cgggccgtcg ccgacggcga ggggtacgtg
     2281 ctgaacggcc ggaagctatg gatctccaac gcgggcgaag ccgggctgtt catcgtgttc
     2341 gccaacgccg atccccaggc cggccatcgg ggaatcaccg cgttcctcgt cgatcgcggc
     2401 acgccgggat tcacggtggg ccggaaggag gacaaggtcg gcgtccgcgc gagcagcacg
     2461 tgcgaactgc tgctggagga ctgccgcctg cccgccagcc aggtgctggg gcggcccggc
     2521 cagggctaca cgatcgcgat cgagctgctg aacgagggcc ggatcggcat cggggcccag
     2581 atgacgggcc tcgcgagggg cgcctaccgc cacgcgatcc agtacgcgcg ggagcgcagg
     2641 cagttcggca ggccgatcgc ggagttccag ctcgtgcggg cgcagcttgc gcggatggcg
     2701 gccgacatcg aggcagcccg cctgctcgtg tacaacgccg cgcggttgtg cgaggcaggc
     2761 aggcccttcg tgaccgagtc ggccatctcg aagctcttcg ccgccgaggc ggccgagcgg
     2821 gtcacctcgg cggccatcaa cgtctacggc ggctgcggct tcgtgcggga cttccccgtc
     2881 gagaagtact ggcgggacgc caagatcggc cagatctacg agggcacgac gaatatccag
     2941 ctgctgacga ttgcgaagca gttgctcggg gaaagacggt agggactcgg ggctcggggc
     3001 tcgggattcg gggctcaggc ctggtcggtc ggggttcgct ggctcggttc gcgtgctgag
     3061 aggtatcctg acttctgaat gctgacttct gactccttct gaatgccatg accactatca
     3121 ctctgcaggt gaaggggatg acgtgcgggg gatgcgagag cgccgtgaag cgcgccctcg
     3181 cgaggctccc cggcgtgggt gcagtggacg cgtcgcacgc cgagcagcgc gtgacggtca
     3241 cgttcgaggc gcaggccgtg tcgccggatg cgatccgagc gaagatctcg gcgctcggct
     3301 acaccgtcct cggctgagcg caggcgcgag gaagcgcggg gccgggggct cggcggcgag
     3361 cgctggaagc ggagggcgcg g
//
LOCUS       DAOSIF010000347         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24574_length_3375_cov_3.808434, whole genome shotgun sequence.
ACCESSION   DAOSIF010000347 DAOSIF010000000
VERSION     DAOSIF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24574_length_3375_cov_3.808434"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLT35_10820"
     CDS             complement(<1..279)
                     /locus_tag="PLT35_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29758.1"
                     /translation="MAALLGVPLLMLAGVTAYYYATFAPIVDERLNGERVKALPRVFA
                     RPLEFRRGQWISMDEFAERLNMLGYAERHGAAQPGEFFRGAGTLTLVAR"
     gene            complement(382..2037)
                     /gene="bshC"
                     /locus_tag="PLT35_10825"
     CDS             complement(382..2037)
                     /gene="bshC"
                     /locus_tag="PLT35_10825"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HOG29759.1"
                     /translation="MSAHPVTSESAAGGICVGLDRLPGFRRLAADYAASPHALSSFFE
                     GHPADADAWRNAIRRTLACPRERDALADIIAAQQRHRGAPPAAVEAAERLRRPGSVAI
                     VTGQQAGLFGGPLLTLLKAVTALKLARRVEADHGTPAVPVFWIEADDHDWEEVASCAV
                     IGADNRLQTIEAAPPPGAGRCPVGSLRLAEEIGIAVDRLTSALPRTEFTEALLDEIRA
                     AYRPGASPADAFGRLVEHALGDEGLAVFDASDPRAKPLARRIFAREAGEPGRTAELAG
                     EAGARLEAMGYHAQVTMRPNSLALFALAGGRLSMRIDGDALLVGEGLAPARNVLAEID
                     ANPAAFSPNVLLRPIVQDAIFPTAAYVAGPNELAYHAQLGRVYEHFAVPRPLVALRLS
                     ATVLEASGLRFLARHGIAFEALAAQDDRVLNGLLESALPPEVESAHQAALLSVETSMR
                     RLAEALPSVDSTLHGAAVSSLGRMVHELRGLREKTLQAAKRNDDVLRRQFARVRAQAF
                     PAGQLQERVVAWVWFLNRHGRGLLRHLLDGLPLDPVCHWVSSG"
     gene            2135..>3375
                     /locus_tag="PLT35_10830"
     CDS             2135..>3375
                     /locus_tag="PLT35_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOG29760.1"
                     /translation="MLASAADQRENLTPGSSYTPPPAFGPLKVLHQIGAGVLGPVFRA
                     READGGRLLAVKAFSLDLTPEQAAQLAAQLKRLAGLELRHACIPAPVAAGVEDSVPYL
                     AVPYVAGESLDAAIRQYGPAPASDAIRLVTHVADALDAAADAGVFHGSLHPRDVLVTP
                     GETHVTGLGVAQALERVGLHGPIRRPYVAPERESGDEWGACADIYSLGAIAYEVLTGR
                     RVLPGADYALPGLTEVRVQNPVALREAIEFAMDPEPGRRPSRAREFAEAFAKALSESI
                     GSGDLRVRQDGSRQRRARSRPPKLPGLDEPLDPALAPPTGEERAAPAKKPLPPPAGRR
                     ITPAATLRSTIHAGSSPPASPGTSARTDAPPEPVEPVDGRAVAVAEEASAPTGQAARS
                     AADESVASAECVAEPVAAGASA"
BASE COUNT          482 a         1212 c         1231 g          450 t
ORIGIN      
        1 ccgtgccacc agtgtgagcg tcccggctcc tcggaagaac tcgcccggct gagccgcgcc
       61 gtggcgctcc gcgtagccga gcatgttcaa ccgctcggcg aactcgtcca tcgagatcca
      121 ctgcccgcgc cggaactcga ggggccgggc gaagacccgt gggagcgcct tgacgcgctc
      181 cccgttgagc cgctcgtcga cgatcggggc gaaggtcgcg tagtagtaag cggtgacgcc
      241 cgcgagcatc aggagcggaa cccccagcag cgccgccacg accgcacggc gccgccggcg
      301 ccgcggaggg ccgggcgggg cgggcagccg gggcccctga cgcgaaggat ctggcgtcgg
      361 catgcgatcg gtctttcgat gctaccccga ggaaacccaa tggcacacgg gatcgagagg
      421 caggccgtcc agcagatgcc ggagaaggcc gcggccgtgc cggttcagga accacaccca
      481 ggcgacgacg cgctcctgga gctggccagc cgggaaggcc tgcgccctga cgcgggcgaa
      541 ctgccgcctc agcacgtcgt cgtttcgctt cgccgcctgc agggtcttct ctctgaggcc
      601 gcgcagctcg tggaccatcc ggcccagcga cgacacggca gcgccgtgca gcgtggagtc
      661 gaccgacggc agcgcctcgg cgagccgtcg catgctcgtt tccaccgaga ggagcgccgc
      721 ctggtgcgcg gactcgacct ccggggggag cgccgactcg agcaggccgt tcagcacgcg
      781 gtcgtcctgt gcggcgaggg cctcgaaggc gatgccgtgc ctggccagga accgcaggcc
      841 ggacgcctcc agcacggtcg ccgagaggcg gagcgccacc agcggccgcg gcacggcgaa
      901 gtgctcgtag acgcgcccca gttgcgcgtg atacgcgagt tcgttcggcc cggcgacgta
      961 ggccgcggtc gggaagatcg cgtcctggac gatcgggcgc aggagcacgt tcgggctgaa
     1021 cgccgcaggg ttcgcgtcga tctcggcgag cacgttgcgg gctggcgcga ggccctcgcc
     1081 gacgaggagc gcgtcgccgt cgatccgcat cgacagccgg cctccggcca gcgcaaagag
     1141 cgccaggctg ttcggccgca tcgtgacctg agcgtggtag cccatcgctt ccagccgggc
     1201 tccggcctct cctgccagct cggccgtgcg gccgggctcg cccgcctccc gggcgaagat
     1261 ccggcgagcg aggggcttgg cccgagggtc cgacgcgtcg aagacggcga ggccctcgtc
     1321 gcccagcgcg tgttcgacga gccgcccgaa ggcgtcggcg gggctcgctc cgggccggta
     1381 cgccgcgcgg atctcatcga gcagggcctc agtgaactcc gtgcgcggca gagcggacgt
     1441 cagccggtcg accgcgatgc caatctcctc agccagccgc aggctcccga ccggacaccg
     1501 acccgcgccc ggcggaggcg ccgcctcgat tgtctgcagc cggttgtcgg cgccaatcac
     1561 ggcacaggac gcgacctcct cccagtcgtg atcatcggcc tcgatccaga agacggggac
     1621 cgccggcgtg ccgtggtcgg cttcgacccg cctggccagc ttgagcgccg tcacggcctt
     1681 gagcagcgtg agcagcggtc cgccgaacaa gccggcctgc tgcccggtga cgatggccac
     1741 cgagccgggg cggcggagcc gttcggcggc ttcgacggcg gcgggcggcg cgccgcggtg
     1801 ccgctgctgg gccgcgatga tgtcggcgag agcgtctcgt tccctgggac atgcgagcgt
     1861 ccgccggatg gcgttgcgcc aagcgtcagc gtcggcagga tgcccctcga agaacgacga
     1921 cagcgcgtgc ggcgacgcgg cgtagtcggc cgccaggcgg cggaatccgg gaaggcgatc
     1981 cagtccgacg cagatcccgc cggcagcgga ctccgaggtc accgggtgtg cactcaccgg
     2041 agagctactc tcgccgaacc atcagcgcgg tgtcaagcca cgcatcggcg tcgtgtaacg
     2101 cgcgcgtcca ggcgtttgat gcgtgggatg ttccatgcta gcatcggccg cggaccagcg
     2161 tgagaacttg acgccaggat cttcgtacac tccgccacct gcgttcggac cgctcaaagt
     2221 cctgcaccag atcggggccg gcgtccttgg gcccgtcttc cgcgcgcgcg aagcggacgg
     2281 ggggcgcctg ctcgccgtga aggcgttctc gctggacctg acgcccgaac aggccgcgca
     2341 gctggccgcg cagctcaagc ggctcgccgg cttggagttg cgccatgcct gcattccggc
     2401 gccagtggca gcgggcgtcg aggactccgt gccctacctg gcggtcccct acgtggccgg
     2461 ggaatcgctg gacgccgcta tccggcagta cggccctgct ccggccagcg acgccatccg
     2521 cctcgtcacg cacgtggccg acgccctcga tgccgccgcc gacgcaggcg tgttccacgg
     2581 gtcgctccat ccccgggacg tcctcgtaac gccaggcgag acgcacgtca cggggctcgg
     2641 cgtcgctcag gctctggagc gcgtcggcct gcacgggccc atcaggcgtc cgtacgtcgc
     2701 tcccgaacgg gaatcgggcg acgagtgggg agcgtgcgcc gacatctact cgctgggcgc
     2761 gatcgcctat gaagtgctga ccggacggcg cgtgctgccg ggcgccgatt acgcgctgcc
     2821 agggctgacg gaggtccgcg tccagaaccc ggttgccctg cgtgaggcca tcgagttcgc
     2881 gatggatccc gagcccggcc gccggccgtc ccgcgcgagg gagttcgccg aggccttcgc
     2941 caaggcgctt tccgagagca tcgggtccgg cgacctccgg gttcgccagg acggctcgcg
     3001 tcagcgaagg gccaggagcc ggccgccgaa gctgccggga ctggacgagc cgctggatcc
     3061 cgccctcgcc ccgccgacgg gagaggagcg tgcggcgcct gcgaagaaac cgctcccgcc
     3121 gcctgcgggc aggcgaatca cgccggcggc gacgcttcgg tcgacgatcc acgccggctc
     3181 ttctcccccg gcaagccctg ggacgtctgc gcggacggac gcaccgcctg aaccggttga
     3241 gccggtcgac ggcagggcgg tcgctgtggc tgaagaggct tcggccccaa ccgggcaggc
     3301 ggcacggtcc gcggctgacg aatcggtggc ctcggccgaa tgcgtcgccg agccggtggc
     3361 cgctggggct tctgc
//
LOCUS       DAOSIF010000348         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24646_length_3368_cov_3.626321, whole genome shotgun sequence.
ACCESSION   DAOSIF010000348 DAOSIF010000000
VERSION     DAOSIF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24646_length_3368_cov_3.626321"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLT35_10835"
     CDS             complement(<1..344)
                     /locus_tag="PLT35_10835"
                     /inference="COORDINATES: protein motif:HMM:NF026035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29761.1"
                     /translation="MSARDAAAPAAAGLALWLTACAAVPPAVQLTPDQRRLHLAAFDQ
                     VWATVRERHFDPDLGGLDWVAVRDELRPRVARASSAGEVRRAIEAMIGRLGQSHFQLL
                     PAEIYRSAEGQQP"
     gene            complement(344..1021)
                     /locus_tag="PLT35_10840"
     CDS             complement(344..1021)
                     /locus_tag="PLT35_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOG29762.1"
                     /translation="MLRNVLRKDLALNAPQLWDLLLWFGYVAYVLYRAPDSVRLAAGG
                     SALVGALIACTIGAREDKLRVSATLASLPVRRRAIVQGRYAVAFAVGAAFLLESRGAP
                     WPRWAAADVGLSFAAAYGGFVAVNLPCHSRWGFGRGNLAAALVLALGIVLADAAGPIV
                     LTGAFGPGDGARASGLPAGQIPAGVGWVAGRLGLVSASVLGFAGNVVLLWLSSEIAVR
                     AARGREF"
     gene            complement(1011..1886)
                     /locus_tag="PLT35_10845"
     CDS             complement(1011..1886)
                     /locus_tag="PLT35_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG29763.1"
                     /translation="MSSDNALQLRGVSKRCRGFALKDVSFTLPRGAIGGLVDPNGAGK
                     TTIVKVVMNLVRRDAGEIRVFGLDNRASEIEVKSRVGFVYDVPGFWDDRTLDAHRRAL
                     GASYSRWSDATFRRLAGEFGLALDAKFKSLSHGAKTRFALAVALSHEADLLIMDEPSA
                     GLDPVFRRDVLKRFSALLQDEGKSVLFSTHITSDLERIADTITFVRAGQIVFSLTRDA
                     LLDGWAVVRADAKVVGDIEPRILKRKRERAFGVEALVSDGDAARRAVGSCGAVDKVTL
                     DDVMVLMAGEGRHAA"
     gene            complement(1883..2299)
                     /locus_tag="PLT35_10850"
     CDS             complement(1883..2299)
                     /locus_tag="PLT35_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG29764.1"
                     /translation="MTDWGPGTLFMTLSLCIELHEGRGARGEGFGARVGDLGLGFQGW
                     GPETLPSIRRLAQDLQISVITTKRAYDELEAEGFIDTVAGKGTFVAAQNADFLREKRM
                     KAVEEQLAAAVADARLMGMTSAQLAEMLRLLWEEGA"
     gene            2566..>3368
                     /locus_tag="PLT35_10855"
     CDS             2566..>3368
                     /locus_tag="PLT35_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29765.1"
                     /translation="MLARRRLAAFAVSFALPAMLSAQSRAGGGDAAPPAADERLLVAM
                     HAVSSHTILDWVKELASDRFEGRLTGTRSYEAAAAWSADLLASWGYLPAGDAGTYYQR
                     FPNPYTLVLPGSDLVLHVPLAGGGEIQKRYVFEQEYYPGSTSDALSATAEAVYAGYGI
                     TAPELGYDDYAGLDVQGKFVVIEPEVPVPPGPDVDTFLRWRPYSFHDYKMRNAAAHGA
                     AGIVYDYFIVNPNAAFVRGLQWVAAGRAVIDDLFAGTGRRHADVVAQIRR"
BASE COUNT          530 a         1245 c         1120 g          473 t
ORIGIN      
        1 ggctgctggc cttctgccga acggtagatc tcggccggaa gcagctggaa gtggctttgg
       61 ccgagccggc cgatcatggc ctcgatggcg cgtcgcacct cgcccgcgct ggacgcgcgc
      121 gcgacacgcg ggcgcagttc gtcccgcacc gcgacccagt cgaggccccc gaggtccgga
      181 tcgaagtgcc gctcacgcac cgtggcccac acctggtcga aggccgcgag gtgcaggcgg
      241 cgctggtccg gcgtcagctg gacggcagga ggaacggcgg cgcacgcagt cagccacagc
      301 gccaggcccg ccgccgctgg cgccgccgcg tctcgagcgc tcatcagaac tcccttcccc
      361 gcgccgcccg cacggcaatc tcggaggaca gccacagcag gacgacgttc cctgcaaacc
      421 ccaggaccga tgccgagacc agccccaggc ggccagcgac ccacccaacg ccggccggga
      481 tctggcccgc aggcagaccg gacgcccgag ccccgtcccc gggcccgaag gcgcccgtga
      541 gaactatcgg cccggccgcg tcggcgagga cgatgccgag ggccagcacg agcgccgccg
      601 cgaggttgcc ccgcccgaat ccccaccgag aatggcacgg gaggttgacg gccacgaagc
      661 cgccataggc ggcggcgaac gagagcccca cgtcggcggc cgcccatcgc ggccacggcg
      721 ctccgcgcga ctcgagcagg aaggcggcgc cgaccgcgaa cgcaacggcg taacgcccct
      781 gcacgatcgc acgccgccgc accggcaggc tggccagcgt ggccgacacg cgcagtttgt
      841 cctcgcgcgc cccgatcgtg cacgcgatca gcgcacccac gagcgcggag ccgccggctg
      901 ccagtcgcac ggagtccggc gcgcggtaca ggacgtacgc cacgtagccg aaccagagga
      961 gcaggtccca gagctgcggc gcgttcagcg ccaggtcctt gcggaggacg ttacgcagca
     1021 tggcgtccct cccctgccat cagcaccatc acatcgtcga gggtgacctt gtcgacagcg
     1081 ccgcaggagc cgaccgcgcg ccgcgctgcg tcgccgtcgg aaaccagcgc ctcgaccccg
     1141 aacgcccgct cccgctttct cttcaggatc cgcggctcga tgtccccgac gaccttcgca
     1201 tcggcgcgga ccactgccca gccgtcgagc agggcgtccc gcgtgagcga aaagacgatc
     1261 tggccggccc gaacgaaggt gatcgtgtcc gcgatgcgct ccaggtcgct cgtgatgtgc
     1321 gtcgaaaaga gcaccgactt gccctcgtcc tgcaggagtg ccgagaaccg cttgagcacg
     1381 tcgcgcctga acaccgggtc gagcccggcg ctcggctcgt ccatgatcag gagatccgcc
     1441 tcgtgcgaca gcgccacggc cagcgcgaac ctcgtcttgg cgccgtgcga cagcgacttg
     1501 aacttcgcat cgagcgccag gccgaactcc ccggccaggc ggcggaaggt cgcatcgctc
     1561 caccgggagt aggacgcacc gagagcccga cggtgcgcgt cgagtgtccg gtcgtcccag
     1621 aagcccggca cgtcgtacac gaagccgacg cgggacttca cctcgatttc cgacgcgcgg
     1681 ttgtcgaggc cgaacacccg gatctcgccg gcgtcgcggc ggacaagatt catgacgacc
     1741 ttcacgatcg tcgtcttgcc cgcgccattc gggtcgacca ggccgccgat ggcgccgcgc
     1801 ggcagcgtga aggacacgtc cttcaacgcg aacccccggc aacgcttcga gacgccccgc
     1861 agttggagcg cgttgtccga gctcatgcgc cctcttccca cagcagccgg agcatctccg
     1921 ccagttgcgc tgaagtcatg cccatcaggc gcgcgtcggc aactgcggcc gcgagctgct
     1981 cttcaactgc cttcatccgc ttctcgcgaa ggaagtcagc gttttgcgcc gccacgaacg
     2041 tgcccttgcc cgcgacggtg tcgatgaagc cctcggcttc gagctcgtcg taggcccgct
     2101 tcgtcgtgat cacgctgatc tggaggtcct gcgccagcct gcgaatcgac ggtagcgtct
     2161 caggccccca accctggaat cctaacccca gatctccaac gcgagccccg aacccctcgc
     2221 cccgggctcc gcgcccctca tgcagctcga tgcacaggct cagcgtcatg aagagcgtgc
     2281 ctgggcccca gtcggtcatg ggagacagcc cccagagcag tacgcacccc gatggagaag
     2341 ccgggcgcgg gaaaggcgcg atgtcgccgg cggcggggcc cccggttgcc tggccgccgt
     2401 catgatgctg ttaacccgtg gcgtgagcgt cttgcgccgg ggttgccttt cgacgggccg
     2461 ctccagcagt cgccaggcag ggcaccaaga gggaggcgcg ggaggacgtt gcacgaccgt
     2521 tcgagtcctc cccgtgatac cctgtggccc cggaggggtc gctccatgct cgcacgccgc
     2581 cgcctggccg cattcgcggt ctcgttcgcg ctgcccgcga tgctctcagc ccagtcgagg
     2641 gctgggggcg gagacgccgc gccgccggcg gcggacgagc gcctgctcgt ggcgatgcac
     2701 gccgtctcga gccacacgat cctcgactgg gtcaaggagc tggcgtcaga ccgattcgag
     2761 ggccgcctca ccggcacccg gtcgtacgag gctgctgcgg cctggtcggc cgatctgctg
     2821 gcgtcgtggg gctatctgcc tgcgggagac gccggcacct actaccagcg gtttccgaat
     2881 ccctacacgc tcgtgctgcc cggctccgat ctcgtgttgc acgtgccgct tgccggcggc
     2941 ggcgagatcc agaagcgtta cgtcttcgag caggagtact atcccggctc gacctccgac
     3001 gccttgtcgg ccaccgccga ggccgtgtac gccggttacg ggattacggc gcccgagctg
     3061 ggctacgacg actacgcggg cctggacgtg cagggcaagt tcgttgtcat cgagcccgag
     3121 gtgcccgtgc cgcccgggcc cgacgtggac acgttcttgc gctggcggcc gtactctttc
     3181 cacgactaca agatgcggaa cgccgcggcc cacggcgccg cgggcatcgt gtacgactac
     3241 ttcatcgtca acccgaacgc ggcgttcgtc agggggctgc agtgggtcgc agccggccgg
     3301 gccgtcatcg acgacctgtt cgccgggacg ggcaggcgcc atgccgacgt cgtcgctcag
     3361 atccggcg
//
LOCUS       DAOSIF010000349         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24859_length_3343_cov_3.045620, whole genome shotgun sequence.
ACCESSION   DAOSIF010000349 DAOSIF010000000
VERSION     DAOSIF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24859_length_3343_cov_3.045620"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLT35_10860"
     CDS             <1..630
                     /locus_tag="PLT35_10860"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOG29766.1"
                     /translation="RSLEACGIEPRRHDVRFEEDNWESPTLGAWGIGWQVLLDGLEIT
                     QFTYFQQVGGLDLEPVSVEITYGLERIAMFLQRVDNVYDLQWAPGVSYREVRLRDEVE
                     QSRYAFGQVAMPREAFVGLHRDLFEKHHGLAGVLLESGLVLPALEQALKCSHLFNVLD
                     SSGGVGVTERTAYILRVRQLAVAVAKAYVESEAQPAPSPAAGARGQHGS"
     gene            620..>1081
                     /locus_tag="PLT35_10865"
     CDS             620..>1081
                     /locus_tag="PLT35_10865"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOG29767.1"
                     /translation="MDRELLIELGCEEIPASWLPPLTRQLGEAAKAGLEEARLETQAP
                     IETFGTPRRLVVRIGRLAERQSDREDVLTGPPAAAAFNPDGSPSPAAAGFARKHGVEP
                     DALERVTTGRGEYLAVRNRVRGKPAADVLPSVLAGILRALPCPKQMRWDARL"
     assembly_gap    1082..1091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1092..2869
                     /gene="glyS"
                     /locus_tag="PLT35_10870"
     CDS             <1092..2869
                     /gene="glyS"
                     /locus_tag="PLT35_10870"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOG29768.1"
                     /translation="DDGRGELLFGRPIRWIVFLYGGRVVPFVIGRSEAARSGLVQDVR
                     SGALTYGHRFLATSGRAGRAIKVRSFQEYRAKLLENFVMLDREERHAKIIRELDAQAR
                     RLGGRVSAAAKKSRLLQEVPDLVEYPSVVAGAFATEFLQLPDEVLTTTMIHHQHFFPV
                     VTDRGSLMPAFLAVVNTEPDDPRAIARNLERTLVARLRDARFFWDADCRTTLDDHLLR
                     LETVLFHKQVGSYLAKARRLEALASWLAAEVLALPDAAAAAGLAGRLAKADLATDLVR
                     ELTELQGEMGGIYAREHGLPEAVWRAIYFHYLPVGVEPSAPPTRSDLGPAAVTWAAVS
                     VADKLDTVTALFAAGERPTGTRDPFGLRRQSHGLLRTLVDLPELAGLEVPIPFGRLLD
                     RARAGVGELPGGGGEPQAWRAAVSSFFLDRLRYLFEQRGFAYDEINAVLGRLTEVPDP
                     LDARRRLEALKAVRGSSDFDALAVAFTRVKNLSRELQGPPTSAVDRLTEPAERALVAE
                     VGARAAAIREAASSRRYEQAFRIASGFRPAVDAFFTDVFVMVDDEELRTQRLSLLRRL
                     YDLLLELADISEIVPAAESQPQTES"
     gene            2875..>3343
                     /locus_tag="PLT35_10875"
     CDS             2875..>3343
                     /locus_tag="PLT35_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOG29769.1"
                     /translation="MAKQTAKTKAKAARATRAAVRKAAPARKAAARPTKPAARAAKAP
                     GAAKKYVYSFGGGKAEGNRTMKDVLGGKGSGLAEMTNAGLPVPPGFTISTAACNLWYA
                     SGRTLPQAIEDEMMAHVRRLEKAAGATFGSTDNPLLVSVRSGAKFSMPGMMDTV"
BASE COUNT          467 a         1135 c         1244 g          487 t
ORIGIN      
        1 cggagcctcg aggcgtgcgg gatcgagccc aggcgccacg acgtgcgctt cgaagaggac
       61 aactgggaat cgcccaccct cggcgcctgg ggcatcggct ggcaggttct actcgacgga
      121 ctggagatca cccagttcac ctacttccag caggtgggcg gcctcgatct ggagcccgtc
      181 tcggtggaga tcacgtacgg cctcgagcgg atcgcgatgt tcctgcagcg cgtcgacaac
      241 gtctacgatc tgcagtgggc gccgggcgtc tcttaccgcg aggtgcggct gcgcgacgag
      301 gtggagcagt cccgttacgc gttcgggcag gtggccatgc cgcgcgaggc gttcgtcggg
      361 ctgcaccgcg acctcttcga gaagcaccac gggctggccg gcgtgctcct ggagagcggg
      421 ctcgtgctcc ccgccctcga gcaggccctg aagtgttccc atctcttcaa cgtcctcgac
      481 tccagcggcg gcgtcggcgt gaccgagcgg acggcctaca tcctgcgggt gcgtcaactc
      541 gcggtggccg tggccaaggc gtatgtcgag tccgaggcgc agccggcgcc gtccccggcg
      601 gcgggggcgc gaggccagca tggatcgtga gctgctcatc gagcttgggt gcgaggagat
      661 cccggcgagc tggctgccgc cgctgacccg gcagcttggc gaagcggcga aggcgggcct
      721 cgaggaggcc cggctggaga cccaggcgcc cattgagacc ttcggcacgc ccagacggct
      781 cgtcgtccgc atcggccggc tcgccgagcg ccagtcggat cgcgaggacg tgctgacggg
      841 cccgcccgcg gccgcggcgt tcaaccccga cggctcgccg agcccagccg ctgcgggctt
      901 cgcgaggaag cacggcgtgg agccggatgc cctggagcgc gtgacgactg gccgggggga
      961 gtacctggcc gtccgcaacc gtgtgcgggg caagccggct gccgacgtcc tgccgagcgt
     1021 cctggccggc atcctgcgcg cgctgccgtg tccgaagcag atgcgctggg atgcgcggct
     1081 gnnnnnnnnn ntggacgacg gccgcggcga gctgctgttc ggccgcccga tccgctggat
     1141 cgtgttcctg tacggcggcc gtgtggtgcc gttcgtgatt ggacgatccg aggccgccag
     1201 gagcggcctg gtgcaggacg tccgttccgg ggccctcacc tacgggcacc gcttcctggc
     1261 gacgagcggc cgcgccggcc gggcgatcaa ggtgcggtcc ttccaggagt accgcgcgaa
     1321 gctcctcgag aacttcgtga tgctcgaccg cgaggagcgc cacgcgaaga tcatccgcga
     1381 actcgacgcc caggcgcgcc gcttgggcgg ccgggtgagc gccgccgcca agaagtcgcg
     1441 gctcctgcag gaggtccccg atctcgtcga atatccgtcg gtggtggccg gcgccttcgc
     1501 caccgagttc ctgcagctgc ccgacgaggt gctgacgacc acgatgatcc accaccagca
     1561 cttctttccg gtcgtcaccg accggggcag cctgatgcct gcgttcctcg cggtggtcaa
     1621 caccgagccc gacgaccccc gcgcgatcgc gcggaacctg gaacgcacgc tagtggcgcg
     1681 gctgcgcgat gcgcggttct tctgggacgc cgactgccgg acgacgctcg acgaccacct
     1741 gctccgcctc gaaaccgtgc tcttccacaa gcaggtcggc agctatctgg ccaaggcgcg
     1801 gaggctcgaa gcgctggcgt cgtggctggc agccgaggtg ctggcgttgc ctgacgccgc
     1861 cgccgcggcc gggctggccg gacggctcgc caaggccgac ctcgccaccg acctggtgcg
     1921 cgagctgacc gagctccaag gcgagatggg cggcatctac gcgagggagc atggcctgcc
     1981 cgaggcggtc tggcgcgcga tctacttcca ctatctgcct gtcggggtgg agccgtcggc
     2041 gccgcccacg cgctcggatc tcgggcccgc ggccgtgacg tgggcggccg tgtcggtggc
     2101 cgacaagctg gacaccgtga cggcgctgtt cgcggccgga gagcggccca ccgggacgcg
     2161 cgacccgttc ggcctgcggc ggcagtccca cgggctgctg cggacgctgg tggacctgcc
     2221 ggagcttgcc ggcctcgagg tcccgatccc gttcggccgg ctgctcgatc gggcccgggc
     2281 tggagtgggg gagttgccgg gcgggggcgg agaaccccag gcgtggcgcg cggccgtttc
     2341 gtcgttcttc ctcgaccggc tccgctacct cttcgagcag cgcgggttcg cgtacgacga
     2401 gatcaacgcc gtcctgggcc ggctgacgga ggtgcccgat ccgctggacg cccgccgccg
     2461 gctcgaggcg ttgaaggccg tccgcgggtc gtcggacttc gatgccctcg cggtggcctt
     2521 cacgcgcgtg aagaacctgt cgcgcgagct tcagggcccg ccgacctccg ccgtggatcg
     2581 gctgacggag cccgccgagc gggcgctcgt cgccgaggtg ggcgcgcgcg cggcggcgat
     2641 ccgtgaggcc gcctcgtcgc gacggtacga acaggcgttt cggatcgcgt ccggcttccg
     2701 gccggcggtt gatgcgttct tcacggacgt gttcgtgatg gtggacgatg aggagttgag
     2761 gacgcagcgg ctctcgctgc tgaggcggct gtacgatctc ctgctcgagc tcgcggacat
     2821 ctcggaaatc gtcccggcgg cggagtcaca accacagacg gagtcatagc gaagatggca
     2881 aagcagactg cgaagacgaa ggcgaaggcg gcgcgcgcga cgcgcgcggc ggtccggaag
     2941 gcggccccgg cgcggaaggc cgcggcgcga ccgacgaagc cggcggccag ggccgcgaag
     3001 gcgccgggtg cggcgaagaa gtacgtgtac agcttcgggg gcggcaaggc cgaaggcaac
     3061 cggacgatga aggacgtgct cggcggcaag ggctcgggcc tcgccgagat gaccaacgcc
     3121 gggctgccgg tcccgccggg cttcaccatc tcgacggcgg cctgcaacct gtggtacgcc
     3181 tcgggccgca cgctgccgca ggccatcgag gacgagatga tggcccacgt tcgcagactc
     3241 gagaaggcgg ccggggcgac gttcggatcg acggacaacc cgctgctcgt gtcggtgcga
     3301 tccggcgcga agttctccat gcccgggatg atggacacgg tcc
//
LOCUS       DAOSIF010000350         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24915_length_3338_cov_2.987207, whole genome shotgun sequence.
ACCESSION   DAOSIF010000350 DAOSIF010000000
VERSION     DAOSIF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24915_length_3338_cov_2.987207"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLT35_10880"
     CDS             <1..694
                     /locus_tag="PLT35_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG29770.1"
                     /translation="PSLDLEDRLDGLEPKFRSLLSLPLKLGGTTLGVVTLYSLRSRAF
                     REEQRQAIELVSDAVAAAFDRAVRTERSRSRRRDAGAPCPSGAGTFCDLLAADEGDEP
                     GRTRAVLFVKCAGDEALMLQAKMAVGRATRVADLIYSPSESALVVLMRDSDPSAGALV
                     SARVASELPADIVPPSDPDSLLRVGFASSPHDGLRWKELVGAAERRAASPAPPDFAPA
                     PASADADERGAQ"
     gene            694..3123
                     /locus_tag="PLT35_10885"
     CDS             694..3123
                     /locus_tag="PLT35_10885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG29771.1"
                     /translation="MPGAGQSAAIRTGWGRYGERSGFSALPPLVQLGVVLTTVAAAVT
                     VVGGVRAFDASPWPILAGLFLLSILTNAVRIELPLVRGGAAISLSYVTSILSILLVGP
                     WPSVPLAVAGAWSGSAFRERDARSWYRTLFSLGTTALSVAAAGLVWRGMAGLLPPAFW
                     SGLASVMGGATAYFFVSTGLVALVIAASAGRETRAAVEARIVARAPGYFIGAAAAFLI
                     AEVVKGERHWWVVAFAAPAFLIYRSYRAHVDRLAAERRQVEEMSDVHLSMIRALALAI
                     EAKDSTTEAQMQRTQVYAEGLARALALAEGDVQAVKMAALLHDIGNLAVPEHILSKPG
                     RLTYDEYDRLKIHPRVGADIIASVPFPYSVAPLVLAHHERWDGRGYPSGLKGEEIPLG
                     ARILAVVDCFTSMLTERPYRPARTYAEAIATLRENGGSTLDPGLVEKFIEILPAIESQ
                     VRRDEPPASERARPESSAEPDGPKTALANIAVAHREERVLRDIGQSLSASLRLSDALS
                     LISTRLISMMPLDACALFLFEPISGLLMCAHVVGSHQDAIRGLTAGSVEGLASQLPMA
                     PASVLGAPVSLQSVAVAPLATEAGTLGAIAVYDVERGVYTADHRRFLARVASHASNVI
                     ANALVFEQAQEQSLTDVLTGLPNRRNYEQHLEQEIARVRRHGGQLSLLVLDMDEFKSI
                     NDEFGHQAGDSALREVARVLRASLRIYDMSARLAGDEFVIVLGDCDAAQAERRRIDIQ
                     RAVAALWVEPIPGRVVRLSASVGAATLPDDADTADGLFETADRRMYRDKARRKRLESQ
                     HRSLVTDALWQ"
BASE COUNT          421 a         1247 c         1185 g          485 t
ORIGIN      
        1 cccctcgctc gatctcgagg accgcctgga cggcctcgag ccgaagttca ggagtctgct
       61 gagcctgccg ctcaaactcg gcggcacgac gctcggcgtc gtgacgctct actccttgcg
      121 gagccgcgcg ttccgcgagg aacagcgcca ggccatcgag ctcgtgagcg acgcggtagc
      181 ggcggctttc gaccgggcgg tgcggacgga gcgcagccga tcccggcgcc gcgacgccgg
      241 tgcgccctgt ccgtccggcg ccggcacctt ctgcgatctg ctcgccgccg acgaaggcga
      301 cgagccgggc cgcacgcgcg cggtgctctt cgtgaagtgc gcgggcgacg aggccttgat
      361 gctgcaggcc aagatggccg tgggccgcgc cacgcgcgtc gcggatctga tctacagccc
      421 gtccgagagc gcgctggtcg tgctgatgcg ggactccgac ccttcggccg gggccctcgt
      481 gagcgcccgc gtcgcatccg agctgccggc cgacatcgtt ccgccctctg acccggattc
      541 gctgctgaga gtcggcttcg cgagcagtcc gcacgacggg ctccgctgga aagagctggt
      601 gggcgccgcc gagcgccggg ccgcgtcgcc ggcgcccccc gacttcgcgc ctgcgccggc
      661 ctcggcggat gccgacgagc gaggagcgca gtgatgccgg gcgcgggcca gtcggctgcc
      721 attcggaccg gttgggggcg ctacggcgag cgctcggggt tcagcgcact tcccccgctc
      781 gtccagctcg gcgtcgtgct gaccacggtg gcggcggccg tcacggtcgt cggcggcgtc
      841 agggccttcg atgccagccc ctggccgata ctggccgggc tgttcctgct gtcgatcctc
      901 acgaacgccg tcaggatcga gctgccgctg gtccggggcg gcgcagcgat ctctctctcg
      961 tacgtgacca gcatcctctc gatcctcctc gtgggcccgt ggcccagcgt gccgctggcc
     1021 gtggccggtg cctggagcgg gagcgcgttc cgcgagcggg acgccaggtc ctggtaccgc
     1081 accctcttct cgctgggcac gaccgcactg agcgtcgcgg cggccggcct cgtgtggcgc
     1141 ggcatggccg ggctgttgcc gccggcgttc tggtccgggc tggcgtcggt catgggaggc
     1201 gcgacggcgt acttcttcgt cagcaccggc ctcgtcgccc tggtgatcgc cgcgtcggcc
     1261 ggccgcgaga cgcgcgccgc cgtcgaagcg cggatcgtgg cgcgcgcgcc gggctacttc
     1321 atcggcgctg ccgcagcctt cctgatcgcg gaggtcgtca agggggagcg ccactggtgg
     1381 gtggtggctt tcgcggcgcc ggcgtttctg atctaccgca gctaccgggc gcacgtggac
     1441 cgcctcgccg ccgagcggcg gcaggtcgag gagatgtccg acgtccatct ctcgatgatc
     1501 cgggcgctcg ccctcgccat cgaggcgaag gacagcacga cggaagcgca gatgcagcgg
     1561 acccaggtct acgcggaagg cctggcccgc gcgctggcgc tggccgaagg cgacgtccag
     1621 gcggtcaaga tggcggccct gctccacgac atcggcaatc tggccgtgcc cgagcacatc
     1681 ctgtcgaaac ccggccgcct gacctacgac gagtacgacc ggctcaagat ccacccgcgg
     1741 gtcggcgccg acatcatcgc gtcggtcccg ttcccgtaca gcgtcgctcc gctggtgctc
     1801 gcgcaccacg agcggtggga cgggcggggc tacccgtccg gcctcaaggg cgaggagatc
     1861 ccccttggcg cgcgcatcct ggcggtggtg gactgcttca cgtccatgct gacggagcgc
     1921 ccctaccggc ccgcgcgcac ctacgccgag gccatcgcca cgttgcgcga gaacgggggc
     1981 tcgacgctgg acccgggcct tgtcgagaag ttcatcgaga tcctgccggc catcgaatcg
     2041 caggtccgcc gggacgagcc tccggcgtcc gagcgcgcgc gccccgagtc gtcggccgag
     2101 ccggacgggc ccaagaccgc cctggccaac atcgccgtgg cgcaccgcga ggagcgcgtg
     2161 ctccgggaca tcgggcagtc gctcagcgcg tcgctccgcc tgtcggacgc cctgtcgctc
     2221 atctcgacgc ggctcatcag catgatgccg ctcgacgcct gcgcgctgtt cctgttcgag
     2281 ccgatctcgg ggctcctgat gtgcgcccac gtcgtcgggt cgcaccagga cgcgatccgc
     2341 gggctgacgg ccggcagcgt cgaggggctc gcgtcgcagt tgccgatggc gcccgcctca
     2401 gtgctcggcg ccccggtcag cctccagtcc gtggccgtcg cccccctcgc caccgaggcc
     2461 gggacgctcg gcgccatcgc ggtgtacgac gtggaacgcg gggtgtacac cgccgaccac
     2521 cgccgcttcc tcgcgcgcgt ggcgagccac gcctcgaacg tgatcgccaa cgccctggtc
     2581 ttcgaacagg cgcaggagca gtcgctcacc gacgtcctca ccgggctgcc caaccgccgg
     2641 aactacgagc agcacctgga gcaggagatc gcccgcgtgc ggcggcatgg cggccagctc
     2701 tcgctgctcg tgctggacat ggacgagttc aagtcaatca acgacgaatt cggccaccag
     2761 gccggcgaca gcgcgctccg cgaggtcgcc cgcgtactgc gcgccagcct gcgcatctac
     2821 gacatgagcg cccggctggc tggcgacgag ttcgtcatcg tcctcggcga ctgcgatgcc
     2881 gcccaggccg agcgccgccg catcgacatc cagcgcgcag tcgcggcgct ctgggtcgag
     2941 ccgattcccg gacgcgtcgt gcggctctcg gccagcgtgg gcgccgccac gctgcccgac
     3001 gacgcggaca cggcggacgg gctgttcgag accgcggacc gccggatgta ccgcgacaag
     3061 gcccgccgca agcgcctgga atcccagcac cggtccctgg tgaccgacgc cctctggcag
     3121 tagccgcgcg cggccgcgcc gggcgcgccg cctcccggtt cggagcgatc acggcctccg
     3181 ctcgggcgga aggcgcggcc gcgcggctct tgtacagcaa accgcccggc cgccgggcgg
     3241 gtccggcgtg ccgggcgcga cggttgccgg cctggccctc ccgggtccag gccccggtcc
     3301 ttctctatcc ctcccagggc aggccctgct tcccgaag
//
LOCUS       DAOSIF010000351         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_24930_length_3336_cov_4.047546, whole genome shotgun sequence.
ACCESSION   DAOSIF010000351 DAOSIF010000000
VERSION     DAOSIF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24930_length_3336_cov_4.047546"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..2565
                     /locus_tag="PLT35_10890"
     CDS             298..2565
                     /locus_tag="PLT35_10890"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOG29772.1"
                     /translation="MPAASPCGPGLHRRSSRATHHPNAAAVALALTLAALSPALAPAA
                     WAGPAALARAGDAFVLQADGGRAWTIGNQHVSFRVGLSSTGALVTQGLARTGSRGEWK
                     MGTAADFTFVAGGRRLTPGQAGFAFVEARVSSADALVELELVFADSASGLRVTRHYTC
                     VQESAAIEAWSVFEAGASGDGLTLGDIGIWRLAVPVTSIDWVNGLQATDEEGGRFTIR
                     HRALEAGGEFAIGSSGRASERFVPVAWFQGAHGTFFGGLRWSGACKLSVTGPAASGLA
                     TIHLTLGDTETTVRRGTAVEGPHGFFGVTGPASADVTRALQAYIRQGVRRGRLFEAPV
                     TYNTWFAYGTRIDERVVRREMEKAARMGVELFTVDAGWYAGGGGVWDFSSGLGDWRAD
                     PERFPSGLRALSDRAHELGMKFGLWIEPERVDLATVDAEGLAGERMLATAGGRFDPSA
                     ANGEAQTAQICLGDAEARAWVLEALVRLIDEARPDYLKWDNNFWINCDRASHGHGAKD
                     GNFAHVKGLYAILGELRRRYPALVIENCSGGGNRLDLGILEFTDSAWMDDVTAPSAHV
                     RHNLQGLGRIFPPAYLLSFVIDAEAESIHDPGSRSLGFRSRMTGMLGLTLRSSDLGDE
                     ELAEMGKEIAAAKEVRAAVPVAAELLLTDQARADGASDLDAVELLSMTTGAAAVFAYA
                     QAGVDAYRVRLAGLDAGLRYRLSTLAGQPLGEATGADLMDAGVEIVNTSDSGSSILLV
                     TPAGPAQAVGAAVRR"
     gene            complement(2633..2827)
                     /locus_tag="PLT35_10895"
     CDS             complement(2633..2827)
                     /locus_tag="PLT35_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29773.1"
                     /translation="MLKLLLWLLLFVLCWPLALLALVLYPLVWLLLLPFRLAGIAVES
                     VFQLLRAVLFLPARLLGARA"
     gene            complement(2974..>3336)
                     /locus_tag="PLT35_10900"
     CDS             complement(2974..>3336)
                     /locus_tag="PLT35_10900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG29774.1"
                     /translation="RVGSPHRGVDLRGAAGTPVVAPAGGRVALADSLYFSGTTVIIDH
                     GLGIHSMLAHLSRIDVAEGAEVAPGDAIGAIGATGRVTGPHLHWTLRLGSVSVDPMSV
                     LDLPGIAKRAHYPIQPPG"
BASE COUNT          461 a         1175 c         1228 g          472 t
ORIGIN      
        1 ccaaagccgg tgccttcggc tcgtcgatga cgggccagcc ggcgctgacg tgggtcgcac
       61 cgcggggcgg cgcggtggtg tgtcccagtt tgcgccggag tgactccgcc gatgccctca
      121 cgaacctcct gcccgcccgg catgaaacgg ccccggtgcc ccggcgctgt ttcgggtcgc
      181 cagcccgtgt tcacatcgcc ctcggcgttc cgacccgcgt tctgccgcgc gggcgcgagc
      241 cggccgcgcc gacatccggc acgatcggca cgcctttggc gcggcctgcc ttcgggtttg
      301 ccggcagcat ctccgtgcgg ccccggcctc caccgacgct cgtcacgagc cacgcaccat
      361 ccgaacgctg ccgcggtcgc cctggcactg acgctggccg ccctttctcc ggcgctggcg
      421 ccggccgcct gggccgggcc ggccgcgctg gcgcgcgcgg gggacgcgtt cgtcctccag
      481 gccgacggcg ggcgggcgtg gacgatcggc aatcagcacg tctcgttcag agtggggctc
      541 agttcgaccg gcgcgctcgt cacgcagggc ctcgcgcgca ccggcagccg cggcgagtgg
      601 aagatgggaa ccgcggctga cttcacgttc gtggcgggag gccgccgcct gacgcccggc
      661 caggccggct tcgcgttcgt ggaagcccgc gtctcgtccg ccgacgcgct ggtggaactc
      721 gaactcgtgt tcgccgactc ggcgtccggc ctccgggtga cccgtcacta cacgtgcgtg
      781 caggagtcgg ccgccattga agcctggtcg gtgttcgaag ccggggcgag cggcgacggc
      841 ctgacgctcg gcgacatcgg catctggcgc ctcgcagtgc cggtgacctc gatcgactgg
      901 gtcaacggcc tgcaggcgac cgacgaggaa ggcggtcggt tcacgatccg gcaccgcgcc
      961 ctggaggcgg gaggcgagtt tgcgatcggg tcgtccggcc gcgcgtcgga gcggttcgtg
     1021 cccgtggcct ggttccaggg cgcgcacggc acgttcttcg gcggccttcg ctggtcgggg
     1081 gcctgcaagc tgagcgtgac gggcccggcg gcaagcggcc tggccacgat ccacctcacc
     1141 ctcggcgaca ctgagacgac agtccgccgt gggacagccg tggaaggccc gcacgggttc
     1201 ttcggcgtga ccggtcccgc ttcggctgat gtcacgcgtg ccctccaggc gtacatccgg
     1261 cagggcgttc gccgcggcag gctgttcgag gcgccggtga cctacaacac gtggttcgcg
     1321 tacgggacgc ggatcgacga gcgcgtcgtg cgccgggaga tggagaaggc ggcgcgcatg
     1381 ggtgtcgagc tcttcacggt ggacgccggg tggtacgccg gcggcggagg cgtctgggac
     1441 ttctccagcg gcctgggcga ctggcgtgcg gatccggagc gcttcccatc cggtctccgg
     1501 gccctgtcgg accgcgcaca cgagctgggc atgaagttcg gcctgtggat cgagcccgag
     1561 cgcgtggacc tggcgaccgt cgatgcggag ggactggccg gggagcgtat gcttgccacg
     1621 gctggcgggc gcttcgaccc gtctgccgcg aacggcgagg cgcagaccgc gcagatctgc
     1681 ctcggcgacg cggaagcccg cgcgtgggtc ctcgaggcgc tcgtgcggct catcgacgag
     1741 gctcgtcccg actacctcaa gtgggacaac aacttctgga tcaactgcga tcgcgccagc
     1801 cacggccacg gggcgaagga cggcaacttc gcccacgtga agggccttta cgcgatcctc
     1861 ggcgaactgc gccgccgcta cccggcgctc gtcatcgaga actgctcggg aggcggcaac
     1921 cgcctggacc tcggcatcct cgagttcacc gacagcgcct ggatggacga cgtgacggcg
     1981 ccgtccgcgc acgtgcgcca taacctgcag ggcctcggcc gcatcttccc tcccgcttac
     2041 ctgctgtcct tcgtgatcga tgccgaggcc gaatcgattc acgatccagg cagccggtcg
     2101 ctcgggttcc ggagccgcat gaccggcatg ctcggcctga cgctgcgctc gtccgacctt
     2161 ggcgacgagg aactcgccga gatggggaag gagatcgcgg ccgcgaagga ggtccgggcg
     2221 gccgtgcccg tcgcggctga actgctcttg accgaccagg cgcgggccga cggggcaagc
     2281 gacctggacg cggtggaact gctgtcgatg acgacggggg cggcagcggt cttcgcctat
     2341 gcccaggccg gcgtcgatgc gtaccgggtg cgcctggccg gcctcgacgc cggcctgagg
     2401 taccggctgt ccacgcttgc cggccagccg ctgggggagg cgacgggagc ggacctcatg
     2461 gacgccggcg tcgagatagt caatacgtcg gattcgggct ccagcatctt gctggtgacg
     2521 cccgccggcc cggcgcaggc tgtcggcgcg gccgtccggc gctagccaca cggcgctggc
     2581 gtgagctgcc ggtccgggcg cgcggctcgc cctggcgacg cgggcctcag ggtcaggccc
     2641 gcgccccgag caagcgggcc ggcaggaaga gcacggcgcg gaggagctga aaaacgcttt
     2701 cgacggcgat gcccgccagc ctgaagggca ggagcagcag ccacaccagc ggatacagca
     2761 ccagcgccag gagcgccagc ggccagcaca gcacgaacag caacagccac aacagcaact
     2821 tcagcaccgc gacctccgtc gcgccgcgcg gcggcgcgtc actcggtact acgagccggg
     2881 aggccggcgg gttctccgca acccggcgcg gagtcgtccg cttcctgagg ccagccgggc
     2941 ggccggttcg ggcgtgtcat cacgccgccg gcctcagccg ggcggctgaa tggggtagtg
     3001 cgccctcttg gcgatgccgg gcagatcgag gaccgacatc gggtccacgc tcaccgaacc
     3061 gaggcggagc gtccaatgca ggtgcggacc ggtgacgcgg ccggtggcgc cgatggcgcc
     3121 gatggcgtcc ccgggcgcca cctccgctcc ttcggcgacg tcgattctcg agaggtgggc
     3181 cagcatcgag tggatgccaa ggccgtggtc gatgatcacg gtcgtgccgg agaagtagag
     3241 actgtcggcg agggcgaccc ggcctcccgc aggcgccacc accggcgtgc cggccgcgcc
     3301 gcgcagatca acgcctcggt gcggagaccc cacccg
//
LOCUS       DAOSIF010000352         3326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25022_length_3326_cov_3.614491, whole genome shotgun sequence.
ACCESSION   DAOSIF010000352 DAOSIF010000000
VERSION     DAOSIF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25022_length_3326_cov_3.614491"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2430
                     /locus_tag="PLT35_10905"
     CDS             <1..2430
                     /locus_tag="PLT35_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29775.1"
                     /translation="GMIFIFGEERPDPATAPRYDIVISPFTFTEALISGYEASFSGKL
                     FGGEATAGLGGSLSLRLAETARDPRPGKPVETDAFTVTRKNGLLGGIAFKPAAKASAT
                     VPIVGEVALSGPAAEIGFTGTVNYGDSHRFHWPLADSEKEPLTVLATHTLLGAMPVFS
                     ETQKLLSFAAWVARDDETSWLNRPEVKSALGKAGLDSRYLEPWRVSHGFDAAFAIKEE
                     LTGFKLGFGRYDTAFVGGQQIINPDTSFTFAGINTGGTLSATVAVGVEDRTLAQQASV
                     SSRLAISASAEAGLKWEFLEGMLTEPWEKAAESIEKSKDSKLVKFVKKNALDSAKAKT
                     FKVLGLEVLISPTEFSRKVSLSAEIQVDIVLDKANNLKPVEVSLTFQGEKPWGPGTKV
                     SGRRYSLTYTVTSEEGIRQVLLNLASIGNILGPQRRVQGQPAGSDQDVGPSSVGEKLF
                     NFVRLILENGTYRETVETGKALNIPLGLGAEVLGEGLKGKVSFTGDQGTSFPIERGVF
                     KGGVQAKLESYADVVPPSAVDNLKNSLFESATSLVRSKLEAARSKLSEMATKLGQVIR
                     HLKGLLRLNGTTEEAAATNMLAHTFERVPGPLPAVPYDPSDYSGQLGKPYYGVGGVYT
                     LTPINMVLSAPAEFVVTYEEADLDGADESTLQLYRWNQARADWDLVASTLDAAANTVT
                     ATITAFGTYAVAPPMPAGEIKWKVTGTAAGGSGGSPSTIVTLTSDPLTRNNGSTVPAG
                     TVVHVLRTFPAGAFLTADALPGVDGLQIVTDADGRLHLQVELPGAPSGVAIDAFSDLG
                     TIRGEVFVPIS"
     gene            2436..3296
                     /locus_tag="PLT35_10910"
     CDS             2436..3296
                     /locus_tag="PLT35_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG29776.1"
                     /translation="MSAACLAAIVLLAAGPAGQGPVSAPGPPAAPPVVVIEAGGLAVT
                     ADELGDAVVEMRRTAEPAALAETLSPEGLERIARRLLERKALAQAARDAGLDRSPDVA
                     RAIAAATDTLLAQSLVEREILSIGESEAALRRYYDGHQEAFRTGARRKAHHLVVATRA
                     EAEAALVEIEAGKPFAEVAAARNTDATKARSGDLGWVSRGVMVKAFDEALFALTKPGQ
                     TSGIVSTSFGYHVIRLDDVDPGRLQPFEAVKAQVRQAARDEAVARLKRELSAKYVPLV
                     NKDALATFGR"
BASE COUNT          565 a         1118 c         1170 g          473 t
ORIGIN      
        1 gggatgatct tcatcttcgg cgaggagcgg cccgaccccg ccaccgcccc gcgctatgac
       61 atcgtcatct cgccgttcac gttcaccgag gcgctgatat ccggctacga ggcgtccttc
      121 tccggcaagc tcttcggcgg agaggccacc gccgggctcg gagggtccct gtccctcagg
      181 ctcgccgaaa cggcgcggga cccccggccg ggcaagccgg tcgaaaccga cgcgttcacc
      241 gtgaccagga agaacggcct gctcggggga attgcgttca agcccgcggc caaggccagc
      301 gcgacggtgc cgatcgtcgg cgaagttgcg ctctccgggc cggcagcgga gattggtttc
      361 accgggacgg tcaactacgg cgacagccac cggttccact ggccgctcgc agactcggag
      421 aaggagccgc tcaccgtgct ggcgacccac acgctgctcg gcgccatgcc ggtcttcagc
      481 gagacccaga agctcctgtc cttcgcggcc tgggtggcca gggacgacga gaccagctgg
      541 ctcaaccggc cggaagtgaa gagcgccctg ggcaaggccg ggctggacag ccggtacctg
      601 gagccttggc gcgtcagcca cgggttcgac gcggccttcg ccatcaagga agaactgacg
      661 ggcttcaagc tcggcttcgg cagatacgac acggccttcg tcggaggcca gcagatcatc
      721 aatccggaca cgtcgttcac gttcgcgggc atcaacaccg gggggacgct gtcagcgacc
      781 gtggccgtcg gcgtggagga tcggacgctc gctcagcagg cgtctgtatc gagcaggctg
      841 gcgatctcgg cttctgccga agccggcttg aagtgggagt tcctcgaggg catgctcacc
      901 gagccctggg agaaggctgc cgagtccatc gagaagagca aagactccaa gctcgtcaag
      961 ttcgtcaaga agaacgccct cgactcggcc aaggccaaga ccttcaaggt gctcgggctc
     1021 gaggtcctga tctcgccgac ggagttctcg cggaaggtct ccctgagtgc ggaaatccag
     1081 gtcgacatcg tcctggacaa ggccaacaac ctgaagcccg tcgaggtcag cctgactttc
     1141 caaggcgaga agccatgggg cccgggcacg aaggtcagcg gccggcgcta cagcctcacg
     1201 tacaccgtga cgtcggagga gggcattcgg caggttctgc tgaacctggc gtcgatcggg
     1261 aacatcctcg ggccgcagcg gcgggtccag ggccagccgg ccgggtcgga tcaggacgtc
     1321 ggtccatcca gcgtgggaga gaagctcttc aacttcgtcc gcctcattct cgaaaacggg
     1381 acgtaccggg agaccgttga gaccgggaag gccctgaaca tccccctcgg tctcggggcc
     1441 gaggtcctcg gcgaagggtt gaagggcaag gtgtccttca ccggcgatca agggacgagc
     1501 ttcccgatcg agcgcggggt cttcaagggg ggcgtccagg cgaaactcga gagctacgcc
     1561 gacgtcgtgc cgccgtccgc ggtggacaac ctgaagaaca gcctcttcga aagcgccacg
     1621 agcctcgtcc gatcgaagct ggaggcggcc aggtcgaagc tgtcggaaat ggccacgaag
     1681 ctggggcagg tcatccggca cctgaagggg ctgctccggc tgaacggcac cacggaagaa
     1741 gccgcggcga cgaacatgct ggcccacacg ttcgaacgcg tccccgggcc gctgccggcg
     1801 gtgccgtacg acccgtccga ctactccggc cagttgggca agccctacta cggtgtcggc
     1861 ggcgtctaca ccctgacgcc catcaacatg gtgctctccg cgcccgctga gttcgtggtc
     1921 acctacgaag aggcggacct cgacggagcg gacgagagca ccctccagct ctaccggtgg
     1981 aaccaggccc gcgccgactg ggatctcgtc gccagcaccc tcgacgcggc ggcgaacacg
     2041 gtgactgcca ccatcaccgc gttcgggacg tacgctgtcg cgccgcccat gcccgccggc
     2101 gagatcaagt ggaaggtcac tgggacggcc gcgggaggca gcggcggatc gccttcgacg
     2161 atcgtgacgc tgacgagcga tccgctgact cgcaacaacg gcagcacggt gcccgcgggc
     2221 accgtcgtgc acgtgctccg gaccttcccc gccggggcgt tcctgacggc tgacgcgctg
     2281 ccgggtgtcg acggcctgca gatcgtcacc gacgccgacg ggaggctcca cctgcaagtc
     2341 gagctgcccg gcgctccgag cggcgtcgcg atcgacgcct tctcggatct gggcacgatt
     2401 cggggagagg tgttcgtgcc gatttcttga gagccatgag cgccgcgtgc ctcgccgcca
     2461 tcgtgctgct cgcggccgga ccggccggcc aggggccggt gtccgccccg gggccgcccg
     2521 ccgcgccgcc ggtggtggtc atcgaggcag gcggcctggc ggtgaccgcc gacgaactgg
     2581 gcgacgcggt cgtcgagatg cggcgcacgg ccgagccggc cgcgctggcc gagacgctct
     2641 cgcccgaggg actcgagcgg attgcgcgcc ggctgctgga gcgcaaggcg ctcgcgcagg
     2701 ccgcgcgcga cgccgggctc gaccgctcgc cggacgtcgc gcgagcgatt gccgccgcca
     2761 cggacacgct gctcgcccag tcgctcgtcg agcgcgagat cctctcgatc ggcgagagcg
     2821 aggcggcgct gagacgctac tacgacgggc accaggaggc gttcaggacc ggtgcgcggc
     2881 gcaaggcgca ccacctcgtg gtggccacgc gggccgaggc cgaagccgcc ctggtcgaga
     2941 tcgaggccgg caaacccttc gccgaggtcg ccgccgcgcg caacacggac gccacgaagg
     3001 cgcgctcggg cgacctgggt tgggtatcgc gcggagtgat ggtgaaggcg ttcgacgagg
     3061 cgctcttcgc gctcacgaag cccggtcaga cgagcggcat cgtctcgacg tcgttcggtt
     3121 accacgtgat ccggctggac gacgtcgatc ccggccggct ccagcccttc gaggcggtga
     3181 aggcccaggt gcggcaggcg gcgagggacg aagccgtggc gcggctcaaa cgggagctgt
     3241 cggcgaagta cgtgcccctg gtgaacaagg acgcgctggc gacgttcggg cgatgaccat
     3301 gcagcttggc accggcaggc ggcgat
//
LOCUS       DAOSIF010000353         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25132_length_3315_cov_3.611963, whole genome shotgun sequence.
ACCESSION   DAOSIF010000353 DAOSIF010000000
VERSION     DAOSIF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25132_length_3315_cov_3.611963"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..1981)
                     /gene="pckA"
                     /locus_tag="PLT35_10915"
     CDS             complement(341..1981)
                     /gene="pckA"
                     /locus_tag="PLT35_10915"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOG29777.1"
                     /translation="MINLLDIKTPARPQAAALKSDYGLEHHGLYNLGGVYWNLPAEGL
                     YEEIVFRREGAITRGGAIVLSTGRHTARSANDKFIVREPSTEDHVWWGQYNRPFAPDK
                     FGELFSRMQGFLQGRDLFVQDCFVGADPEYRMPVRIITEFAWHSLFARNMFIVPRTLE
                     EYRRHVPDFTVICCPAFKGIQQIDATASDTVIVLDFSQRLCLIGNTGYGGEIKKAVFT
                     VMNYLMPLQGVFPMHSSANVGAGGDVAVFFGLSGTGKTTLSADPGRGLIGDDEHGWSD
                     NGVFNFEGGCYAKVIQLSESAEPQIYATTHRFGTILENVVFDPVTRMIDLDDPALTEN
                     TRASYPLEFIGNALAEKRAGHPKNVIFLTCDASGVMPPIARLSVDQARYQFISGYTSK
                     IAGTEVGLGKEPEITFSTCFGAPFMVHQPDFYARLLKEKVLRYGADCWLVNTGWVGGP
                     YGVGKRISIRHTRAMLNAALGGQLRDVEYQTDPIFGFQVPRTCPGVPDQVLDPAASWP
                     NRELYVQRYRQLAARFIDNFKKFDTVEAREVEQAGPRL"
     gene            complement(1978..3195)
                     /locus_tag="PLT35_10920"
     CDS             complement(1978..3195)
                     /locus_tag="PLT35_10920"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG29778.1"
                     /translation="MSVSELAGAIIESPTLRLNEEARLLRERGEPVIHLGIGEPKNKT
                     PITAILSSAAKLSQGDVKYCPSDGLPSLKKAIIRYTEEAYDKVVAPENVIVSSGAKQA
                     IFNVLYTLLNPQDEVIILAPYWVSYPEMVKMVYGVPVIVTPEDGSFHPRMADIERAVS
                     SSTKAIIVNSPNNPSGVVYREAFIAEIVEFCERKKIYLIMDDIYHKLVFDGRAAPPCY
                     RFTTKPLEDSRLIVINGIAKLYGMTGFRIGWCVAPRRIVSVINNVQAQTTSCASPVMQ
                     AGAEGALLGLQSFVESLRLTIQNNRDVVMRELGSFNGVKTQKPDGTFYCLPDFRAYSQ
                     DSVELSGFLLKKALVVTVPGREFGMEGHLRLSFAGTVKDVTEGIARIKWALDPTSPND
                     IYIGDKRLIRDWR"
BASE COUNT          485 a         1105 c         1151 g          574 t
ORIGIN      
        1 tgtggctgtc gcttgtggtg gttcggcatg ggttcggctc gaagcgtcga tgcggttggt
       61 ccgtgacgag cggtcgttac tcgcggggtg gaagggcccc gccgcccgga tagacgcggg
      121 ggttgcctgg ccccctcact atagagcagg ccattcgcgc cgtcaactcg ctcacccggc
      181 gcgccgggag tgcccgtgcg ggccgggcgc gggcgcacga gagccgagcg cgtcagcggc
      241 gcccgcaggc ccttgaggcg gggaactcga taaggcgctc cgaggcgccg tggcgccgtg
      301 accgatgccg ggctccggcg cctggtgcct tgctcgtgtc ctacagccgc ggccccgcct
      361 gctccacctc gcgcgcctcc accgtgtcga acttcttgaa gttgtcgatg aagcgcgccg
      421 ccagctggcg gtaccgctgg acgtacagct cgcggttcgg ccacgaggcc gccgggtcga
      481 gcacctggtc gggcacgccg gggcaggtcc tgggcacctg gaacccgaag atcgggtccg
      541 tctggtactc gacgtcgcgc agctgcccgc cgagcgcggc gttgagcatg gcccgcgtgt
      601 ggcggatgct gatgcgcttg ccgacgccgt acgggccgcc cacccagccg gtgttgacga
      661 gccagcagtc ggcgccgtac cgcaggacct tctccttgag gaggcgggcg tagaagtcgg
      721 gctggtggac catgaacggc gcgccgaagc aggtgctgaa cgtgatctcg ggctccttgc
      781 cgaggccgac ctcggtgccg gcgatcttgg aggtgtagcc cgagatgaac tggtagcggg
      841 cctggtcgac cgagagcctc gcaatcggcg gcatgacgcc cgacgcgtcg caggtgagga
      901 agatcacgtt cttcgggtgc cccgcccgct tctcggcgag cgcgttgccg atgaactcga
      961 gggggtacga cgcccgggtg ttctcggtca gcgccgggtc gtcgaggtcg atcatccggg
     1021 tgaccgggtc gaagacgacg ttctcgagga tcgtgccgaa ccggtgcgtc gtcgcgtaga
     1081 tctgcggctc cgccgactcg gacagctgga tgaccttcgc gtagcagccg ccctcgaagt
     1141 tgaagacgcc gttgtcgctc cagccgtgct cgtcgtcgcc gatgagcccg cggcccgggt
     1201 cggccgagag ggtcgtcttg cccgtgcccg acaggccgaa gaacaccgcg acgtcgccgc
     1261 ccgcgccgac gttggccgac gagtgcatcg ggaacacgcc ctgcagcggc atgaggtagt
     1321 tcatgaccgt gaacacggcc ttcttgatct cgccgccgta ccccgtgttg ccgatgaggc
     1381 acagccgctg gctgaagtcg aggacgatga ccgtgtccga cgcggtcgcg tcgatctgct
     1441 ggatgccctt gaaggccggg cagcagatga cagtgaagtc cggcacgtgg cgccggtact
     1501 cctcgagcgt cctcgggacg atgaacatgt tgcgcgcgaa gaggctgtgc caggcgaact
     1561 cggtgatgat gcgcaccggc atgcggtact cggggtcggc gccgacgaag cagtcctgga
     1621 cgaacaggtc gcgcccctgc aggaagccct gcatgcgcga gaagagctcg ccgaacttgt
     1681 ccggcgcgaa ggggcggttg tactgccccc accagacgtg gtcctccgtc gagggctcgc
     1741 gcacgatgaa cttgtcgttc gccgaccgcg cggtgtgccg gcccgtgctg aggacgatcg
     1801 ctccgccgcg ggtgattgcg ccctcgcgcc ggaagacgat ctcctcgtag aggccctcgg
     1861 ccgggaggtt ccagtacacg ccgcccaggt tgtagaggcc gtgatgttcg agcccgtagt
     1921 cgctcttgag cgccgccgcc tgcggccgcg cgggcgtctt gatgtcgagg aggttgatca
     1981 tcgccagtcc ctgatcaggc gcttgtcgcc gatgtagatg tcgttggggg aggtcgggtc
     2041 gagcgcccac ttgatccggg cgatgccctc ggtcacgtcc ttcaccgtgc cggcgaagct
     2101 cagccgcagg tgcccctcca tgccgaactc gcgccccggc acggttacca ccagggcctt
     2161 cttcagcagg aaccccgaca gctcgacgga gtcctggctg taggcccgga agtcgggcag
     2221 gcagtagaag gtgccgtccg gcttctgcgt cttcacgccg ttgaacgagc ccagctcgcg
     2281 catcaccacg tcgcggttgt tctggatcgt caggcgcagc gactcgacga agctctgcag
     2341 gccgagcagc gcgccttccg cccccgcctg catgaccggc gacgcgcacg aagtggtctg
     2401 cgcctggacg ttgttgatca cggagacgat ccgccgcggc gcgacacacc agccgatccg
     2461 gaaccccgtc atgccgtaga gcttcgcgat gccgttgatc acgatgaggc gcgagtcctc
     2521 cagcggcttc gtcgtgaagc ggtagcacgg cggcgccgcg cgcccgtcga agacgagctt
     2581 gtggtagatg tcgtccatga tcagatagat cttcttccgc tcgcagaact cgacgatctc
     2641 cgcgatgaac gcctcccggt acaccacgcc gctcgggttg ttcgggctgt tcacgatgat
     2701 ggccttcgtc gaggagctga ccgcccgctc gatgtccgcc atgcgcggat ggaagctccc
     2761 atcctcgggc gtgacgatga cgggcacgcc gtagaccatc ttgaccatct ccgggtagct
     2821 cacccagtac ggcgcgagga tgatcacttc gtcctgcggg ttgagcagcg tgtacagcac
     2881 gttgaagatc gcctgcttgg cgcccgagga cacgatgacg ttctccggcg cgacgacctt
     2941 gtcgtacgcc tcctcggtgt accggatgat cgccttcttc agcgacggca ggccgtcgga
     3001 cgggcagtac ttcacgtcgc cctggctcag cttcgccgcc gaactcagga tggcggtgat
     3061 gggcgtcttg ttcttgggct ccccgatccc gaggtgaatg accggctcgc cgcgctcccg
     3121 cagcagccgc gcctcctcgt tcagccgcag cgtcggcgac tcgatgatgg ctccggccag
     3181 ctcactgacg ctcatggtgg ctcctgtctg tgcgtgttcg gaaggtcggg ccgcaccgcg
     3241 gcccgcgggg cggcgccccc actatggcct agggccgcgc gcgcttctgg cggaggcgcg
     3301 cctccaccag gtcgg
//
LOCUS       DAOSIF010000354         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25187_length_3310_cov_3.496774, whole genome shotgun sequence.
ACCESSION   DAOSIF010000354 DAOSIF010000000
VERSION     DAOSIF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25187_length_3310_cov_3.496774"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLT35_10925"
     CDS             complement(<1..724)
                     /locus_tag="PLT35_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29779.1"
                     /translation="MSTRRIGIVMNGVTGRMGANQHLMRSIVAIGRDGGVQCADGTIV
                     EPVPLLVGRNASKLEALAAQAGGASWTTSLDAALSDRSCEVYFDAQTTDRRAAAVRRA
                     IAAGKHVYCEKPAAATMAEALELYHLARRAGLKHGVVQDKLWLPGIRALKQLVDEGFF
                     GRILSVRGEFGYWVFEGETIRSQRPSWNYRAEEGGGIVRDMFAHWRYVLDSVFGRVSA
                     VACLGATHVERRWDESGRTYDCT"
     gene            complement(740..1174)
                     /locus_tag="PLT35_10930"
     CDS             complement(740..1174)
                     /locus_tag="PLT35_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG29780.1"
                     /translation="MTIVNLDAVEGRRFPAGRRTKHIVGGLSPIQSCHFALGLVTLDA
                     DGGQIPWHSQDQQEVYFIVEGTGEMCLGAERATVGAGQAVAIPSGVFHQITNVGPTPL
                     RLLYCYGPAGDAAHWRQELDGTLPRAGVEAPALPDGAHAQRA"
     gene            1692..2861
                     /locus_tag="PLT35_10935"
     CDS             1692..2861
                     /locus_tag="PLT35_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG29781.1"
                     /translation="MTATKVERVSVQRQVELSGTLLSLDQARVSSEAAGLVQSVPIQI
                     GTEVRPGSILVQLDPRERQFAMESAESALRQTEAQLGMSPGQAVPPADDAIASVRSAA
                     ATRDDARANFERASRLMSRGLLAPADLETAQSRLKVAEASYQQVFDQARALKASLMDR
                     RASYELAKKRLADTSIRAPVAGIVSERLVQPGEFIQTNTPVAVVVQIHPLKLRTALQE
                     RYAGLISQRLPVEFRVEPFPGETFRGEIAHVSPSVDQATRTFVVEAIVENPDRRLKPG
                     FFAKGVLTTNVDANVPAVSEDAVATLAGVSSVFVIENNVIRQQEVALGVKQGKLVEIV
                     NGLKGDETLAASGLSNLATGTPVRVLTPGSAPPPDGGGPPGAGRGARGGARGGQR"
     gene            2858..>3310
                     /locus_tag="PLT35_10940"
     CDS             2858..>3310
                     /locus_tag="PLT35_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG29782.1"
                     /translation="MKLADVSVDRPVFAVMMSVALIVLGAFSYRHLGLDLMPKTDYPT
                     VMVMTQLPGASAEEVESQITKRIESVVNSINGVDELRAGSDQGFSRVSITFVLERDIE
                     SATQDVRDKVATIVGQFPTDTRPSYISKIDPDSQPILTLVVSANRTQKE"
BASE COUNT          476 a         1132 c         1212 g          490 t
ORIGIN      
        1 cagtgcagtc gtaggtacgg cccgattcgt cccaccgccg ctccacgtgg gtcgcgccga
       61 ggcacgccac ggcgctgacc cggccgaaca cgctgtcgag cacgtagcgc cagtgggcga
      121 acatgtcccg gacgattccg ccgccctctt ccgcgcggta gttccatgac ggccgctgcg
      181 agcggatcgt ctcgccctcg aagacccagt agccgaactc ccccctgacg gacaggatcc
      241 gtccgaaaaa cccctcgtcg acaagctgct tcagcgcgcg gatgccgggg agccagagct
      301 tgtcctgcac gaccccgtgt ttgaggccgg cgcggcgggc gaggtggtac agctcgagcg
      361 cctcagccat ggtggccgcg gccggcttct cgcagtagac gtgcttgccg gcggcaatcg
      421 cacgacggac ggcagcggcc cgccggtcgg tcgtctgcgc gtcgaagtag acctcgcacg
      481 accgatcgga cagagcggcg tccaggctgg tcgtccacga cgcgccgccg gcctgcgccg
      541 ccagggcctc cagcttggaa gcgttccggc ccacaaggag cggcacgggc tcgacgatcg
      601 ttccgtcggc gcactggacg ccgccgtcgc ggccgatggc gacgatggat cgcatgaggt
      661 gctggttagc gcccatgcgg ccggtgacgc cgttcatgac gatgccgatg cgccgggtgc
      721 tcatgggcag cctccccgct caggcgcgct gagcgtgcgc gccgtcgggc agcgccgggg
      781 cctccacgcc cgcccgcggc agcgtcccat cgagttcctg ccgccagtgc gccgcgtctc
      841 ccgcgggccc gtagcagtag agcaggcgga gcggcgtcgg gcccacgttc gtgatctgat
      901 ggaagacccc ggacggaatc gcgacggcct ggccggcgcc cacggtcgcg cgctccgcgc
      961 cgaggcacat ctcgcccgtg ccctcgacga tgaagtagac ctcctgctgg tcctggctgt
     1021 gccacgggat ctgaccgccg tccgcgtcga gcgtcacgag accgagggcg aagtggcacg
     1081 actggatcgg cgacaggccc ccgacgatgt gcttcgtccg ccggccggcc gggaaacggc
     1141 ggccctcgac ggcgtcgaga ttgacgatgg tcatttcccc tcccggcgat ctcaggcgcg
     1201 gcgacggccg ccagcccggc ccggaccccc tgccgccggg cctcgcggtt cttgcgatcc
     1261 tcccctcgaa gcgacagtcc gctcgcgcgc gccgagcggg cgcggcgccc gctgcgggac
     1321 ggtcagcgcg gacattgtag cggatgcggg ggattgtcac ggttttgtcg ccgtctgtaa
     1381 gcggaacggg gtgatgtcgt agtagcctta agagtgtctg tctgaccgcc ctccgggagg
     1441 gtgtcgcggc tctgcggtgg ggcgggatcg ggaaatgggg ccggaaggcc aggctgcggc
     1501 tcgggaacta tatccacatg ctgcgtgtag cttttcgtcg tgttccagcg tcggtcgggc
     1561 tcttgattgg cgcgtctctg tgtctcgccg gggcgtcggc cgcgtgcagg gggtcctcga
     1621 acgggccggg cggcgggtcc gccggccggg gggcgcaggg cggccggggg ggaccgcgcg
     1681 aggcggtcaa cgtgacggcg accaaggtcg agcgcgtgtc cgtgcagcga caggtggagc
     1741 tgtcgggcac cctgctgtcg ctcgatcagg cccgggtcag ttccgaggcg gccggcctcg
     1801 tgcagagcgt tccgatccag atcggcaccg aggttcggcc cgggagcatc ctcgtccagc
     1861 tggatcctcg cgagcgccag ttcgcgatgg agtcggccga gagcgccctt cggcagacgg
     1921 aggcgcagct gggcatgtcc ccgggccagg cggtgccgcc cgccgacgat gcgattgcct
     1981 cggtgcgctc ggcggccgcc acccgggacg acgcccgcgc caacttcgag cgcgcgtcgc
     2041 ggctgatgag ccgcggcctc ctggcgccgg cggacctcga gaccgcgcag tcgcggctga
     2101 aggtcgccga ggcgtcgtac cagcaggtct tcgatcaggc gcgtgcgctg aaggccagcc
     2161 tgatggaccg ccgcgcgtcg tacgagctgg cgaagaagcg gttggcggac acctcgatcc
     2221 gcgcgcccgt ggcgggcatc gtgtcggagc ggctggtcca gcccggggag ttcatccaga
     2281 ccaacacgcc ggtggcggtc gtggtgcaga ttcatccgct caagctccgc accgcgctgc
     2341 aggagcggta cgctggcttg atctcgcagc ggctcccggt ggagttccgc gtggagccgt
     2401 ttccgggcga gacgttccgc ggcgagatcg cgcacgtgag cccctcggtg gaccaggcga
     2461 cgcgcacctt cgtggtcgag gcgatcgtcg agaaccctga ccggcgcctg aagcccgggt
     2521 tcttcgcgaa gggcgtgctg acgacgaacg tggacgcgaa cgtgccggcg gtgagcgagg
     2581 acgcggtggc cacgctggcg ggcgtgtcct cggtgttcgt catcgagaac aacgtcatcc
     2641 gccagcagga agtcgcgctc ggggtgaagc aggggaagct cgtcgagatt gtgaacgggc
     2701 tgaagggcga cgagacgctc gccgccagcg ggctgagcaa tctcgcgacg gggacgccgg
     2761 tgcgggtcct gacgccggga agcgcgccgc cgccggacgg gggcggcccg ccgggggccg
     2821 gccggggcgc caggggcggt gcgcgtggag gccagcggtg aaactcgcgg acgtcagcgt
     2881 cgatcgcccg gtcttcgccg tcatgatgag cgtggcgctc atcgtgctgg gcgcgttttc
     2941 ctaccgccac ctcgggctcg atctcatgcc caagaccgac tacccgacgg tcatggtcat
     3001 gacgcagctc ccaggcgcga gcgccgagga ggtcgagtcg cagatcacca agcgcatcga
     3061 atcggtggtc aacagcatca acggcgtgga cgagctccgg gccggctccg accagggctt
     3121 ctcgcgcgtg tcgatcacct tcgtgctgga gcgcgacatc gaatccgcca cgcaggacgt
     3181 gcgcgacaag gtcgccacca tcgtcggcca gtttcccacc gacacccggc cgtcgtacat
     3241 cagcaagatc gacccggatt cgcagcccat cctgacgctc gtcgtctcgg ccaaccggac
     3301 ccagaaggag
//
LOCUS       DAOSIF010000355         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25197_length_3309_cov_3.325753, whole genome shotgun sequence.
ACCESSION   DAOSIF010000355 DAOSIF010000000
VERSION     DAOSIF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25197_length_3309_cov_3.325753"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLT35_10945"
     CDS             complement(<1..838)
                     /locus_tag="PLT35_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOG29783.1"
                     /translation="MTLNTLTEKAQEAVLDAQQRAERASHAQIDPEHLLTALLGQADG
                     VAPEALRKAGLDPRALASQAEALLARLPQAHGGAAPGASPRLRAVLNAALDEARSMKD
                     EFISTEHLLLAVAGEQGRSAAARLLASAGVTRERILEALQAIRGSQRVTDQNPEGKYQ
                     ALERYGRDLTDLARKGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVRGDVPEGLKRKRIVGLDMGALVAGAKFRGEFEERLKAVLKEVADADGLVVL
                     FID"
     gene            complement(849..1847)
                     /locus_tag="PLT35_10950"
     CDS             complement(849..1847)
                     /locus_tag="PLT35_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOG29784.1"
                     /translation="MQFKDYYATLGVAKTATEKEIKQAYRKLARKLHPDVNPGDKSAE
                     SRFKSINEAYEVLGDPDKRRKYDELGANWRQYEQAQQQGGPGGFGAGGWTVNVGGGPA
                     GFRTMTEDEMRRLFGEEDPFSDFFHAFFGGAATAGERGPSRQGRGGRARRGRDVEQDL
                     ELTLEEAFQGALRRFFAIKHGGHTRSVDVRIPAGVGDGSRVRVAGEGESGSPGSPPGD
                     LYLRVHLAPHPRFERRGKDLYVRVQVPVTTAVLGGEAHVHAIDGGRLRLRVPPATQNG
                     QVLRLKGHGMPAVGRADDRGDLYATIDVTLPRSLDAEQQRLYEALRHHEEGSTRSS"
     gene            complement(1862..2206)
                     /locus_tag="PLT35_10955"
     CDS             complement(1862..2206)
                     /locus_tag="PLT35_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG29785.1"
                     /translation="MTQGEELFFISMAARELGMHPQTLRKYERLGLVRPTRSIGSVRL
                     YTRSEIQRLRAIKHLVDEAGINLAGVQRLLGVAEAVERIRPLLSDEALGQAVGRRSLA
                     REVERLMEVLGL"
     gene            complement(2326..>3309)
                     /locus_tag="PLT35_10960"
     CDS             complement(2326..>3309)
                     /locus_tag="PLT35_10960"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HOG29786.1"
                     /translation="SRLDGVRVADLAWQHGQDSLSCFAKQDDKRHFTVDGRAFVGFRV
                     RSRVAVAVGDPVGEAAAIPGVIAGFVEHCRVNDWVPVFYEASARYLELYGAQGLRWFK
                     VGEEALLDLATWSLGGGAVAKVRQFVNKIRREAPDLAVEEYQRHVPNPDLDGQLEDVS
                     SEWLAGKKGGEMGFNLSVFSVEHLRDRRTFVARRSHGTVEAFVTWLPYRAGRAAVLDA
                     MRHRPSAPPGVMDLLIAESALAFKREGLEAVSLAVAPMADASGAQAASTYDRAVRLIF
                     EHFSQVYGYRTLFQFKKKFAPRWEPRYLVFPRPDLLPRIAYALIGVHYARS"
BASE COUNT          458 a         1198 c         1149 g          504 t
ORIGIN      
        1 cgtcgatgaa gagcaccacc aggccgtcgg cgtcggcgac ctccttcagc acggccttca
       61 gccgctcctc gaactcgccg cggaacttgg cgccggccac cagcgcgccc atgtcgaggc
      121 ccacgatgcg cttgcgcttc agcccctccg ggacgtcgcc gcgcacgatc cgctgggcca
      181 gcccctcgac gatggccgtc ttgccgacgc cgggctcgcc gatgaggacc gggttgttct
      241 tggtgcggcg cgatagcacc tggatgacgc ggcggatctc ctcgtcgcgg ccgatgaccg
      301 gatcgagctt gcccttgcgg gcgagatccg tcaggtcgcg gccgtagcgc tcgagggcct
      361 ggtacttgcc ctccgggttc tggtccgtca cgcgctgcga cccgcggatg gcctgcaggg
      421 cctcgaggat gcgctcccgc gtcacgccgg ccgacgccag caggcgcgcg gcggcggagc
      481 ggccctgctc gcccgccacc gcgagcagca ggtgctccgt gctgatgaac tcgtccttca
      541 tgctccgcgc ctcgtccagc gccgcgttca gcacggcccg cagccggggt gaggcgccgg
      601 gagcggcgcc gccgtgcgcc tgcggcagcc gggcgagcag ggcctcggcc tgggaggcga
      661 gcgcgcgggg atcgagcccg gccttgcgca gtgcctcggg ggcgacaccg tccgcctggc
      721 cgagcagggc ggtcagcagg tgttcgggat cgatctgggc gtgactggcg cgttcggcgc
      781 gctgctgcgc gtcgagcacg gcttcctggg ctttttccgt caacgtgttg agcgtcatgg
      841 cgtccggact agctcgatcg ggtcgagccc tcctcatggt gtctgagcgc ctcgtagagg
      901 cgctgctgtt cggcgtccag cgatcgcggc agcgtcacgt cgatcgtggc gtagaggtcc
      961 ccgcggtcgt ccgcgcggcc gacggcgggc atgccgtgtc ccttcaggcg gagcacctgg
     1021 ccgttctgcg tcgccggcgg caccctgagc cgaaggcgcc cgccgtcgat ggcgtgcacg
     1081 tgcgcctcgc cgccgagcac ggcggtcgtc accgggacct gcacgcgcac gtacagatcc
     1141 ttgccgcggc gttcgaagcg cgggtggggc gccaggtgca cgcggagata caaatcgccc
     1201 ggaggcgagc ctggcgagcc gctctcgcct tcgcccgcca cccgcaccct cgagccgtca
     1261 ccgacgccgg cgggaatgcg cacgtccacg cttcgcgtgt ggccgccgtg cttgatggcg
     1321 aagaaccgcc gcagggcgcc ctggaacgcc tcctccagcg tcagctcgag atcctgctcg
     1381 acgtcccgcc cgcggcgggc tcgcccgccc cgcccctgcc gcgacggccc ccgctcgccg
     1441 gcagtcgcgg ccccgccgaa gaacgcgtgg aagaaatccg agaacgggtc ctcctcgccg
     1501 aagaggcgcc gcatctcgtc ctcggtcatc gtccgaaagc cggccgggcc gccgccgacg
     1561 ttcaccgtcc agccgcctgc gccgaagcct cccgggccgc cctgctgctg cgcctgctcg
     1621 tactgccgcc agttcgcgcc gagttcgtcg tacttgcggc gcttgtccgg gtcgcccagg
     1681 acttcgtacg cctcgttgat cgacttgaac cgcgactcgg cggacttgtc acccgggttg
     1741 acgtcgggat gaagcttgcg ggcgagtttg cggtacgcct gcttgatctc cttctccgtc
     1801 gccgtcttgg cgacgccgag cgtggcgtag tagtccttga actgcacggc cgtctcaggg
     1861 cctagaggcc cagcacctcc atcaagcgct cgacttcgcg ggccaggctc cggcgcccca
     1921 cggcctgccc gagagcctcg tccgagagaa ggggccggat ccgctcgacc gcctcggcca
     1981 cgcccagcag gcgctgcacg ccggcgaggt tgatgccggc ctcgtccact aggtgcttga
     2041 tggcgcgcag gcgctggatc tcggagcgcg tatacaggcg gacgcttccg atggagcgcg
     2101 tgggccgcac caggccgagg cgctcgtact tccggagcgt ctgcgggtgc atgcccagct
     2161 cccgcgcggc catgctgatg aagaacaact cctcgccttg agtcatatca acgagcccat
     2221 gtatacgaac cgagtatagc acttgatacg ttccatatca agggccgccc gatgccgtct
     2281 tgggcgccgc gctcgtccga ggggcggcga gggccgcgcc ccgcgtcagg accgcgcgta
     2341 gtggacgccg atcagagcgt aggcgatccg cggaaggagg tcgggccgcg ggaagaccag
     2401 gtaccgcggc tcccagcgcg gggcgaactt cttcttgaac tggaagagcg tccggtagcc
     2461 gtacacctgc gagaagtgct cgaagatgag ccgcaccgcg cggtcgtacg tcgacgcggc
     2521 ctgcgcgccg ctggcgtcgg ccatcggagc gacggccagg ctcaccgcct cgaggccctc
     2581 gcgcttgaag gcgagggcgg attcggcgat gaggaggtcc atgacgccgg gaggcgcgct
     2641 cggccggtgg cgcatcgcgt cgagcacggc cgcgcgcccc gcgcggtaag ggagccaggt
     2701 cacgaaggcc tccacggtgc cgtgtgatcg gcgcgccacg aacgtccgcc ggtcccggag
     2761 gtgctcgacg gaaaacaccg agaggttgaa gcccatctcg ccgcctttct tgccggcgag
     2821 ccactcgctc gacacgtcct cgagttggcc gtcgagatcc gggttcggca cgtggcgctg
     2881 gtactcctcg actgccaggt ccggcgcttc gcggcggatc ttgttcacga actgccgcac
     2941 cttggcgacc gcgccgccac caagcgacca ggtcgccaga tcgaggaggg cttcctctcc
     3001 caccttgaac cagcgcaagc cctgcgcgcc gtagagctcg aggtagcggg cggacgcctc
     3061 gtagaacacc ggcacccagt cgttcacgcg gcagtgctcg acgaagcccg cgatgacgcc
     3121 gggaatcgcc gctgcctctc ccaccggatc gccgaccgcg acggcgacgc ggctcctcac
     3181 gcggaacccg acgaaggcgc ggccgtccac ggtgaagtgg cgtttgtcat cctgcttcgc
     3241 gaagcaggac agcgaatcct ggccgtgctg ccatgccagg tcggccaccc tgacgccgtc
     3301 gaggcggga
//
LOCUS       DAOSIF010000356         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25255_length_3304_cov_3.049554, whole genome shotgun sequence.
ACCESSION   DAOSIF010000356 DAOSIF010000000
VERSION     DAOSIF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25255_length_3304_cov_3.049554"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2601
                     /locus_tag="PLT35_10965"
     CDS             <1..2601
                     /locus_tag="PLT35_10965"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOG29787.1"
                     /translation="PFHVTSTDWQQSMRELAGVLTAFGSPSRPAEVGGEGTFEGVLFN
                     AIWDPRIEGFFAGAGLTFWDVDWGRGESAVVIEHLYADVKDAEVRKGAGTLRATGRFS
                     LGYPRRDRGEELNAVIRISDWDLADFRHAFEFDAYPWDGRVHGELHLWGAYEEPFGFG
                     KVTLAPLAVYEETLTSATASLRFDGPGVWLDGIEMRKGATGIVRGGARAEWLGSYSFN
                     VDGQNRPVESVDLFAFPDAPLSGRLDFTASGGGKALDVSYDADFRMRDLYVKDEGIGD
                     VKGRLELRGDDMNVSFDAISSRLTASGAGKVTLLGDYPGDVTLRLTDASLDPYARVFM
                     PGLSPFASAVGSGTVRVSGTLLSLDNVVAHVHVERLALKLFDYVLQNDGGLDASLEQG
                     ILRVGRFRLSGEDTRLTLSGNVDLVQGILGMRAEGSANLGILQAVTSNVRGSGRADVT
                     ADFTGTLEEPRVGGSAAIEGGRLRHMWLPHAIDGINGRVRFDGESVRFDDIAATIGRG
                     DVRLGGRVDLAGLWPSRFDITATGEGMELRYPEGFRSVIDADLGLRGTVDDLVLGGTV
                     LVRRGELRRDLELGTGLADLAAGGGGAGIAAPQAPAPTLPLRFDLRLSAPSTIEIDNK
                     LARLTASADLRLRGTYARPVLEGRAEVQRGELWFEGKRYVVSRGSAEFTNPSKIDPYF
                     DVEAETRVRAPGQTYRVTFRVAGTTSAFEYDLSSDPPLPEVDVLSLLLGDVGSTQDAE
                     LRALRTPDEAEQNLLIARSARLLASPISSGVQKAVEQTFGLDSVQIAPFFVDPSQSAG
                     RFSPGARLTIGKRISDRIYLTYSRSLSSAARDQVILLEYDQSDRLAWIVTQNEDRTYA
                     LDLRVRHVFR"
     gene            2598..>3304
                     /locus_tag="PLT35_10970"
     CDS             2598..>3304
                     /locus_tag="PLT35_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29788.1"
                     /translation="MRPPPPARAFALAAALAAWTASPSAAQDAGLPVGRTISDVSVVS
                     EGRPVLDAQVLELLALRKGSPLRMEDARATIVRAMGLGRYLDVRVSAEPDGDAVRLSV
                     DLVPLPAQGRLVFEGDLGLSESAIRGAVTERFGPDPPRGRADEVARVVGDLLRDQGYL
                     RATVTPRDARPGETGFGDLVFNVVCGPRALVRSISFRPEAGEEVEQLRLRLSIRPGVV
                     FNRVELRRQLDAATEAWR"
BASE COUNT          440 a         1180 c         1182 g          502 t
ORIGIN      
        1 ccgttccacg tcaccagcac cgactggcag cagagcatgc gggagctggc cggcgtgctc
       61 accgccttcg gatcgccgtc gcgcccggct gaagtgggcg gggagggcac cttcgagggc
      121 gtgctcttca acgccatctg ggacccgcgc atcgagggct tcttcgccgg cgcaggcctg
      181 acgttctggg acgtggactg gggccggggc gagagcgccg tcgtcatcga gcacctgtac
      241 gccgacgtga aggacgcgga ggtccggaag ggcgcgggga cgctgcgcgc gaccggccgc
      301 ttctcgctgg gctatccgcg cagggaccgc ggcgaggaac tcaacgccgt gatccggatc
      361 agcgactggg acctggcgga cttccgccac gcgttcgagt tcgacgccta tccctgggac
      421 ggccgcgtgc acggcgaact ccacctctgg ggcgcgtacg aagagccgtt tggattcggc
      481 aaagtcacgt tggctccgct ggcggtctac gaggagacgc tgaccagcgc cacggcgtct
      541 ctgcgcttcg acggaccggg ggtgtggctc gacggcatcg aaatgcggaa gggggccacc
      601 ggcatcgtgc gcggcggcgc ccgcgccgag tggctgggct cgtactcgtt caacgtggac
      661 gggcagaaca gacccgtcga gtcggtcgac ctctttgcct tccccgacgc gccgctctcg
      721 ggccggctcg atttcacggc cagcggcggg ggcaaggccc tggacgtctc ctacgacgcc
      781 gacttccgga tgcgcgacct ctacgtcaag gacgagggca tcggcgacgt gaaaggacgc
      841 ctcgagctgc ggggcgacga catgaacgtc tcgttcgacg ccatctcgtc ccgcttgacc
      901 gcatccgggg ccgggaaggt gacgctgctc ggcgactacc ccggcgatgt cacgctgcgc
      961 ctgaccgacg cctcgctcga tccctacgcc cgggtcttta tgcccggcct ctcgccgttc
     1021 gcctcggcgg tggggtcggg gacggtgcgg gtcagcggca cccttctgag cctcgacaac
     1081 gtcgtcgcgc atgtccacgt cgagcgcctg gcgctgaagc tcttcgacta cgttctgcag
     1141 aacgacggcg gcctcgacgc cagcctggag cagggcatcc tgcgggtcgg gcgcttccgg
     1201 ctgagcggcg aggacacgcg cctcaccctg tcgggcaacg tggacctggt gcaggggatc
     1261 ctcgggatgc gcgccgaggg cagcgcgaac ctcgggatcc tccaggccgt gacgtccaac
     1321 gtccgcggat ccggccgtgc ggacgtcacc gcggatttca ccggcacgct cgaggagccg
     1381 cgcgtcggcg gcagcgccgc catcgagggc gggcggctgc ggcacatgtg gctgcctcac
     1441 gccatcgacg gcatcaacgg gcgcgtccgg ttcgacgggg agagcgttcg cttcgacgac
     1501 atcgcggcga ccatcggccg cggcgacgtc cgccttggcg ggcgcgtgga tctcgccggc
     1561 ctctggccgt cgcgcttcga catcaccgcg accggagagg gcatggagct gcgctacccg
     1621 gagggcttcc gctcggtcat cgacgcggac ctgggcctgc gcggcaccgt cgatgacctc
     1681 gtgctcggcg gcacggtcct cgtccggcgc ggcgagctgc ggcgcgacct ggagctcggg
     1741 acgggcctcg cggaccttgc cgccggggga ggcggcgccg gcatcgccgc gccccaggcc
     1801 ccggcgccga cgctgccgct gcgcttcgac cttcggctgt cggcgccgtc cacgatcgag
     1861 atcgacaaca aactcgcccg cctcaccgcc agcgccgatc tgaggctccg cgggacctac
     1921 gcccggcccg ttctcgaggg ccgcgccgag gtccagcgcg gcgagctctg gttcgagggc
     1981 aagcgctacg tggtgtcgcg gggatcggcg gagttcacga acccgtcgaa gatcgacccg
     2041 tacttcgacg tggaggccga gacgcgcgtc cgggcgcccg gccagaccta ccgcgtgacg
     2101 ttccgcgtcg ccggcacgac atcggcgttc gagtacgatc tgtcgtccga cccgccgctt
     2161 cccgaagtgg acgtcctgtc gctgctgctt ggcgacgtgg gctccacgca ggacgccgag
     2221 ttgcgcgccc tcaggacgcc ggacgaggcc gagcagaacc tgctgatcgc gcgctcggcg
     2281 cggctgctgg ccagcccgat ctcctcgggc gtccagaagg ccgtcgagca gaccttcggg
     2341 ctggactccg tgcagatcgc gcccttcttc gtcgacccga gccagtcggc agggcgcttc
     2401 tcgccgggcg cccgcctcac catcggcaag cgcatttccg accgcatcta cctgacgtac
     2461 tcgcgcagcc tgtcgtcggc cgcacgcgac caggtgatcc tgctcgaata cgatcagagc
     2521 gaccgtctgg cgtggattgt cacccagaac gaggatcgga cctacgcgct cgacctgcgg
     2581 gtccgccacg tgttccgatg agaccgccgc caccggcccg ggcgttcgcg cttgccgccg
     2641 cgctcgcggc atggaccgcg tcgccgtcgg ccgcccagga tgccggcctg ccggtcggcc
     2701 gcacgatctc ggacgtctcc gtcgtatcgg aggggcggcc ggtcctcgac gcccaggtgc
     2761 tggagctgct cgcgcttcga aagggctcgc ccctccggat ggaagacgcc cgggccacga
     2821 tcgtccgcgc gatgggcctc ggccgctatc tggatgtgag ggtgtcggcc gagcccgacg
     2881 gcgacgccgt ccgcctctcc gtcgacctgg tgcccctgcc ggcccagggc cgcctcgtgt
     2941 tcgagggcga tctcggcctg tccgagtccg cgatccgcgg agcggtcacc gagcgcttcg
     3001 gccccgaccc gccccgggga agggccgacg aggtcgcgcg cgtggtcggc gaccttctca
     3061 gggatcaggg ctacctgcgg gccaccgtga cgccgcgaga cgcccggccg ggtgagaccg
     3121 ggtttggcga tctggtcttc aatgtcgtgt gcggcccgcg agcgctggtg cggtcgatct
     3181 cgttccgccc ggaagccggc gaggaggtcg agcagctgcg cctccgcttg agcatccggc
     3241 ccggagtcgt tttcaaccgc gtcgagctcc gccggcagct ggatgccgcg acggaggcgt
     3301 ggcg
//
LOCUS       DAOSIF010000357         3302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25269_length_3302_cov_3.255313, whole genome shotgun sequence.
ACCESSION   DAOSIF010000357 DAOSIF010000000
VERSION     DAOSIF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25269_length_3302_cov_3.255313"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="PLT35_10975"
     CDS             <1..1507
                     /locus_tag="PLT35_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOG29789.1"
                     /translation="QPFMEDEQRRSGAEGRPRGTVVLATVKGDVHDIGKNIVGVVLAC
                     NNYKVVDLGVMVPASRLLAEAERQQAGIIGLSGLITPSLDEMVGVAAEMQRRGLSLPL
                     LIGGATTSRQHTAVRIAPAYGGPVVHVPDASRVIEVVANLLSAGRRAAFEAGNRADQD
                     ALRTRHRARAARPLRPFPEAQARRLRLDWTREDIPTPAFTGRRVVADVSLGKLAEYID
                     WTFFFSAWGLKGRFPAILEHAEYGKAARDLYDNARRMLRTFVEEGSLRARGVYGFWPA
                     ASDGDDIVLWRDRERSVEAARFPMLRQQEIVEEDRPQLSLADFVAPLDAGVPDFVGGF
                     AVTAGLGADEMAARHAAAHDDYSGIMVKALADRLAEAFAEYLHARVRREWGYGAGEQL
                     SYDDLVEERYRGIRPAFGYPACPDHTEKGRLFDLLDAPAIGMKLTESFAMWPAASVSG
                     LYFASPHARYFGVGRIGRDQVEAYAARKRMTAAEVERWLAPNLGYAPDENA"
     gene            complement(1523..3268)
                     /locus_tag="PLT35_10980"
     CDS             complement(1523..3268)
                     /locus_tag="PLT35_10980"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG29790.1"
                     /translation="MSSRTCRSVVLLLVTGLVSALAAACRPAAPAPPRPEPASLVIRN
                     GRIVTVDPARPEAQAMAIRGDTIAAVGSEAEIAPFVGPATTLIDLKGALAVPGFIDSH
                     AHFNGIGQSKLGLDLMAATSWDEILAMVADAAKHAQPGEWILGRGWHQEKWTARPDPN
                     VEGFPTHASLDRVSPDNPVFLTHASGHASIANARAMALAGVTRATASPPGGDILKDGS
                     GEPAGVFREAASGLISRARDAARAKMTPDELEAEALEIIRLADRECLSKGITTLHTAG
                     ESFAAIDRFKRVIDGGRLGIRLYAMVGEPNARMKDALAAYRVIGYGRGHLTVRAVKRF
                     MDGALGSRGAWLLEPYADLPGATGLNSTPPAELRESAELAIANGYQLCIHAIGDRANR
                     EVLNLYEETLRAHPEARDVRWRVEHAQHLHPSDIPRFGTLGVVAAMQGIHCTSDAPYV
                     LARLGPQRAEEGAYVWQKLMRSGATIANGTDAPVEAVDPLPSYFATVTRQLKDGSVFF
                     GSQKMSRLEGLKSYTINGAYAGFEEGVKGSLAPGKLADVTILSKDILAVPDGEILSAK
                     VLYTIVGGQVLYTAQ"
BASE COUNT          462 a         1192 c         1181 g          467 t
ORIGIN      
        1 ccagccgttc atggaagatg agcagcgccg gagcggggcc gagggccgcc cgcggggcac
       61 ggtcgtcctc gcaacggtca agggcgacgt ccacgacatc ggcaagaaca tcgtgggcgt
      121 cgtgctggcg tgcaacaact acaaggtggt cgatctgggc gtgatggtgc ccgcctcacg
      181 cctgctggcc gaggccgagc ggcagcaggc cgggatcatc gggctgtcgg ggctgatcac
      241 gccgtcgctc gacgagatgg tgggcgtcgc ggcggagatg cagcggcgcg ggctctcgct
      301 gccgctgctc atcggcggcg cgacgacgag ccgccagcac acggcggtca ggatcgcccc
      361 ggcgtacggc ggccccgttg tccacgtgcc ggacgcgtcg cgcgtcatcg aggtcgtggc
      421 gaacctgctg agcgcggggc gcagggccgc attcgaggcc gggaatcgcg cggaccagga
      481 cgcgctgcgg acccggcacc gcgcgcgcgc cgcgcgcccc ctcaggccgt tcccggaggc
      541 gcaggcgcgc cggctccgcc tggactggac gcgcgaagac atcccgacgc ccgccttcac
      601 ggggcggcgc gtcgtggcgg acgtgtccct cggcaagctg gccgagtaca tcgactggac
      661 cttcttcttc tcggcctggg gcctcaaggg gcgcttcccc gccatcctcg agcacgccga
      721 gtacggcaag gccgcccgcg atctgtacga caacgcccgc cgtatgctgc ggaccttcgt
      781 cgaagaggga tctctgcgcg ccaggggcgt ctacggcttc tggcccgccg cgagcgacgg
      841 cgacgacatc gtgctctggc gcgaccgcga gcgcagcgtc gaggcggccc ggttcccgat
      901 gctgcggcag caggagatcg tcgaggagga ccgcccgcag ctgtcgctgg cggacttcgt
      961 cgcgccgctc gacgccggcg tgccggactt cgtcggcggg ttcgccgtca cggccggcct
     1021 gggcgccgac gagatggcgg cgcgccatgc cgccgcgcac gacgactaca gcggcatcat
     1081 ggtgaaggcg ctggcggacc gcctggccga ggcgttcgcc gagtacctcc atgcgcgcgt
     1141 ccggcgcgaa tggggctacg gcgccggcga gcagctcagc tacgacgacc tcgtggagga
     1201 gcgctaccgc gggatccgtc cggccttcgg ctatccggcc tgcccggacc acacggaaaa
     1261 agggcggctg ttcgacctgc tcgacgcacc ggccatcggc atgaagctga ccgagtcgtt
     1321 cgcgatgtgg ccggcggcca gcgtgagcgg gctgtacttc gccagcccgc acgccaggta
     1381 cttcggcgtg gggcgcatcg ggcgcgacca ggtggaggcc tacgccgcac gcaagcggat
     1441 gacggccgcc gaggtcgagc ggtggctcgc cccgaacctc ggctacgcgc cggacgagaa
     1501 cgcctgacct cgcgcggcgc gcctactgcg ccgtatagag cacctggccg ccgacgatgg
     1561 tgtagagcac cttcgcagac aggatctcgc cgtccggcac cgcgaggatg tccttcgaca
     1621 ggatcgtcac gtcggcaagc ttgccgggag cgagcgaccc tttgactccc tcctcgaacc
     1681 cggcgtaggc cccgttgatc gtgtaggact tcaggccctc cagccgggac attttctggg
     1741 agccgaagaa caccgacccg tccttcagct gccgcgtgac cgtcgcgaag tagctcggca
     1801 gcggatcgac ggcctccacg ggagcgtcgg tgccattcgc aatggtggcg ccagaccgca
     1861 tcagcttctg ccagacgtac gcgccctcct ccgcccgctg cgggccgagg cgcgccagga
     1921 cgtacggcgc gtccgacgtg cagtggatgc cctgcatggc ggcgacgacg ccgagcgtcc
     1981 cgaaccgcgg aatgtccgac gggtgcagat gctgcgcatg ctcgacgcgc cagcgcacgt
     2041 ccctcgcctc cggatgggcc ctgagcgtct cttcgtacag gttcagcacc tcgcggttcg
     2101 cgcggtcgcc gatggcgtgg atgcagagct ggtagccgtt ggcgatggcc agctccgccg
     2161 actcccgcaa ctcggccggc ggcgtgctgt tcagccccgt cgcgccaggc aggtccgcgt
     2221 acggctcgag cagccaggcg ccccgggagc ccagcgcgcc gtccatgaag cgcttcacgg
     2281 ccctcaccgt caggtgcccg cggccgtagc caatcacccg gtaggccgcc agcgcgtcct
     2341 tcatgcgggc gttcggctcc cccaccatcg catacagcct gatgcccagt ctgccgccgt
     2401 cgatgacgcg cttgaaacga tcgatcgccg cgaacgactc gcccgccgtg tgcagcgtgg
     2461 tgatgccctt cgacaggcac tcgcggtccg ccaggcggat gatctcgagc gcttcggcct
     2521 ccagctcgtc cggcgtcatc ttcgcccgcg ccgcgtcgcg ggcccggctg atgagcccgg
     2581 aggctgcttc gcgaaagacc ccggcaggct cgccggaacc gtccttcagg atgtccccgc
     2641 ccggcgggct cgccgtcgcg cgcgtcacgc cggccagggc catcgcccgg gcgttcgcga
     2701 tgctggcgtg cccgctcgca tgcgtcagga acacggggtt gtcgggcgag acccggtcga
     2761 gcgaggcgtg tgtggggaac ccctccacgt tcgggtccgg gcgcgccgtc cacttctcct
     2821 gatgccagcc gcggccgagg atccactcgc cgggctgcgc gtgcttggcg gcgtcggcca
     2881 ccatggcgag gatctcgtcc cagctcgtcg cggccatgag atccaggccg agcttcgact
     2941 ggccgatccc gttgaagtgg gcgtggctgt cgatgaagcc cggcacggcc agggcgccct
     3001 tcagatcgat cagcgtcgtg gccggaccca cgaacggcgc gatttcagcc tcgctgccaa
     3061 cggccgcgat ggtgtcgccg cggatggcca tggcctgggc ctcgggccga gccgggtcca
     3121 ccgtcacgat gcggccgttt cgaatcacga gcgacgcggg ctccggacga ggcggcgccg
     3181 gcgcggccgg ccggcaggcc gccgcgagcg cggaaaccag gccggtgacg agcagcagaa
     3241 ccacggagcg gcaggtgcgg ctggacacgg ttcgcctcct cggcccgcgc tccgcgcccc
     3301 gg
//
LOCUS       DAOSIF010000358         3298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25294_length_3298_cov_4.079864, whole genome shotgun sequence.
ACCESSION   DAOSIF010000358 DAOSIF010000000
VERSION     DAOSIF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25294_length_3298_cov_4.079864"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLT35_10985"
     CDS             complement(<1..696)
                     /locus_tag="PLT35_10985"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG29791.1"
                     /translation="MSEAGRTPGAREMQVPMLDLQAQYRPIRDAILEAVMRVCDSQRF
                     ILGPEVEGLERELAAYLGVEHAVGVSSGTDALLAAMMALGIGPGDEVVTSTYSFFATA
                     GCVSRLGARPVFVDIDPVSCNIDPGAIAAAVTPRTKAIVPVHLYGQSAELDAILAVAS
                     RAGVPVIEDAAQAIGATYHGRKVGGFGLAGCFSFFPSKNLGAFGDGGLVTTNDEALAA
                     SVRRLRMHGADRQY"
     gene            complement(722..1291)
                     /locus_tag="PLT35_10990"
     CDS             complement(722..1291)
                     /locus_tag="PLT35_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29792.1"
                     /translation="MLDNKKIVAGLAVLAVVVAAAAFWMLRQGGGAVPIDLVAQLDDA
                     QKQTTWAEPGDAPFTVSDVTLQGETHRAIFAPPFSRIRWKVEVPRRGTLELYFGMRED
                     AWEAEGNGAQFRVGVSDGRTYEEYLKEVVNPQARERDRRWLSASIDLSAYEGQLVEIN
                     LNTDPGPPRDDRDRRNDFALWGEPRLVGR"
     gene            complement(1371..1865)
                     /gene="smpB"
                     /locus_tag="PLT35_10995"
     CDS             complement(1371..1865)
                     /gene="smpB"
                     /locus_tag="PLT35_10995"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOG29793.1"
                     /translation="MSAQDAERRRERKRAERNIAENRKAAYDYHLVETFEAGIALVGT
                     EVKAIREGRVNLRDSYARAERGEIYVHGVHIGAYSARGYADHEPLRPRKLLLHRAEIR
                     KLIGRTTERGMTLVPVRMYFKDGRVKVAVSLAKGKKSHDRRETIRRREAERETRAAVK
                     ERGR"
     gene            complement(1862..2770)
                     /gene="pdxA"
                     /locus_tag="PLT35_11000"
     CDS             complement(1862..2770)
                     /gene="pdxA"
                     /locus_tag="PLT35_11000"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOG29794.1"
                     /translation="MAITLGDPAGIGPEIAAKAASDPRVAAACAPVLVGPPAAPGVRP
                     GCVSAEAGRAAYEAVVEAVRAAMAGEVDAVATAPISKEAFALAGLPWRGHTELLAHLT
                     GAPSVAMMFHADALRVVLATIHEPLADVPRLLTQELLERVIRLAARELPRFGFADPKL
                     AVCGLNPHAGEHGVLGVEEQRAIAPAIEACRAGGIAVAGPFPADTLFVAAARGGFDAV
                     IACYHDQGLIPVKLLAFGKAVNVTLGLPIVRTSVDHGTAFDIAGRGVADPGSLVEAVL
                     LAARLAAGADGAGGAAAAAGGKDRRP"
     gene            complement(2782..>3298)
                     /gene="ricT"
                     /locus_tag="PLT35_11005"
     CDS             complement(2782..>3298)
                     /gene="ricT"
                     /locus_tag="PLT35_11005"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOG29795.1"
                     /translation="LRHEQREREAFRLCVMKIRERGLAMKLARGEQLFDGSRLVFYFT
                     SEERVDFRELVRELASEFKTRIELRQIGVRDEARMLSGYGTCGRPLCCSSWLKAFEPV
                     SIKMAKQQDLALNPSRLSGLCGRLKCCLRYELNHAVAAPARDDACPGGGSAARPAAGC
                     GNCAGARGRGK"
BASE COUNT          430 a         1214 c         1193 g          461 t
ORIGIN      
        1 gtactggcgg tccgccccgt gcatgcgcag gcgccgcacg gacgccgcaa gcgcctcgtc
       61 gttcgtggtc acgaggccgc cgtcgccgaa ggcgccgagg ttcttgctcg ggaagaacga
      121 gaagcacccc gccaggccga agccgcccac cttcctgccg tggtaggtcg cgccgatggc
      181 ctgggccgcg tcctcgatga cgggaacgcc ggcccgggac gcgacggcca ggatcgcatc
      241 gagctcggcg ctctgcccgt acaggtgcac cgggacgatg gccttcgtcc gcggcgtgac
      301 ggcggcggcg atggcgcccg gatcgatgtt gcagctgacc gggtcgatgt ccacgaacac
      361 gggcctggcc ccgagcctcg acacgcaccc cgccgtcgcg aagaacgagt acgtgctggt
      421 gaccacctcg tctccggggc cgatcccgag cgccatcatg gcggccagca gcgcgtcggt
      481 gcccgacgag acgccgacgg cgtgttcgac gccgaggtac gccgcgagct cccgttcgag
      541 gccctcgacc tcgggcccga ggatgaaccg ctggctgtcg cagacgcgca tgaccgcctc
      601 gaggatcgcg tcgcggatcg gccggtactg ggcctgcagg tccagcatcg gcacctgcat
      661 ctcgcgcgct ccgggcgtgc ggcctgcttc gctcatgtga agcctcccgg cggcggcggc
      721 gtcagcgacc gacgaggcgg ggctcgcccc agagggcgaa gtcgttgcgg cggtcgcggt
      781 cgtcacgcgg cgggccgggg tcggtgttga ggttgatctc gaccagctgc ccctcgtagg
      841 ccgacaggtc gatcgacgcc gacagccagc ggcggtcgcg ctcgcgggcc tgcggattga
      901 cgacttcctt gaggtactcc tcgtaggtgc gcccgtccga cacgccgacg cggaactgcg
      961 cgccgttgcc ctcggcctcc cacgcgtcct cccgcatgcc gaagtacagc tcgagggtgc
     1021 cgcggcgggg aacttccacc ttccagcgga tccgcgagaa cggcggcgcg aagatggccc
     1081 tgtgggtctc gccctggagc gtgacgtcgc tcacggtgaa cggcgcgtcg ccgggctcgg
     1141 cccaggtcgt ctgcttctgg gcgtcgtcca gctgcgcgac caggtcgatc ggaaccgcgc
     1201 ctccgccctg gcgcagcatc cagaacgcgg cggccgccac gacgacggcg agcacggcca
     1261 gcccggcgac gatcttcttg ttgtccagca cgggagacat ccctccttcc acgcgtcagg
     1321 ctcgaagcgt accatccggc ccgaacgggc cgcaagcggc gagggcggcc tcagcggccg
     1381 cgttccttca cggcggctct cgtctcgcgc tccgcctcgc gccgccggat cgtctcgcgc
     1441 ctgtcgtgcg acttcttgcc cttcgcgagg ctgacggcga ccttcacgcg tccgtccttg
     1501 aagtacatcc gcacgggcac cagcgtcatg ccgcgctcgg tcgtcctgcc gatgagcttg
     1561 cggatttcgg ctcggtgcag cagcagcttg cgcgggcgca gcggctcgtg gtcggcgtag
     1621 ccgcgggcgc tgtaggcgcc gatgtgcacg ccgtggacgt agatctcgcc cctctcggcg
     1681 cgggcgtagc tgtcgcgcaa gttgacccgc ccctcccgga tggccttcac ctccgtgccg
     1741 accagcgcga tgccggcctc gaaggtctcg acgaggtggt agtcgtaggc ggccttgcgg
     1801 ttctcggcga tgttgcgctc ggcgcgcttc cgttcgcgcc tccgttcggc gtcctgcgcg
     1861 ctcacggccg gcggtccttc ccgcctgcgg cggcggcggc gccgccggcc ccgtccgcgc
     1921 cagccgccag cctggccgcc aggagaacgg cctccaccag gctgccgggg tcggcgacgc
     1981 cccggccggc gatgtcgaag gccgtgccgt gatcgaccga cgtgcgcacg atcggcaggc
     2041 ccagcgtcac gttcacggcc ttgccgaagg ccagcagctt cacggggatg agcccctggt
     2101 cgtggtagca ggcgatgacg gcgtcgaagc cgccgcgggc cgccgcgacg aagagcgtgt
     2161 cggccggaaa cggcccggcg acggcgatcc cgccggcccg gcacgcctcg atggcgggcg
     2221 cgatggcgcg ctgctcttcg acgccgagca cgccgtgctc gccggcgtgg ggattcaggc
     2281 cgcagacggc cagcttcgga tccgcgaagc cgaaccgcgg cagctcgcgc gccgcgagcc
     2341 ggatcacgcg ctcgagcagc tcctgcgtca ggagccgcgg cacgtcggcg agaggctcgt
     2401 ggatggtggc cagcacgacg cggagcgcgt cggcgtggaa catcatcgcc accgacggcg
     2461 ccccggtcag atgggccagc agttccgtgt ggccgcgcca gggcagaccg gccagggcga
     2521 acgcctcctt gctgatcggg gccgtcgcga cggcgtccac ctcgccggcc atcgccgcgc
     2581 ggacggcctc cacgacggcc tcgtacgccg cgcggcccgc ctcggccgac acgcagccgg
     2641 ggcggacgcc cggcgcggcg ggcgggccga cgagcacggg cgcgcacgcc gcggcgacgc
     2701 ggggatccga cgcggccttc gcggcgatct cgggcccgat gccggccggg tcgcccaacg
     2761 taatcgccac gcggggccgc gtcattttcc gcgtccgcgg gccccggcgc agttgccgca
     2821 tcctgccgcg gggcgggcgg cgctgccgcc gccagggcag gcgtcgtcgc gggcgggggc
     2881 cgccacggcg tgattcagtt cgtagcgcag gcagcacttc aggcggccgc agagcccgga
     2941 cagccgcgag gggttcagcg ccaggtcctg ctgcttggcc attttgatcg acacgggctc
     3001 gaaggccttc agccagctgc tgcagcagag gggacggccg cacgtgccgt agccggagag
     3061 catcctggcc tcgtcgcgga cgccgatctg cctcagctcg atgcgggtct tgaactcgga
     3121 cgcgagctcg cgcaccagct cgcggaaatc gacgcgctcc tcggacgtga agtagaacac
     3181 gaggcgcgag ccgtcgaaga gctgctcgcc gcgcgccagc ttcatggcga ggccgcgctc
     3241 ccggatcttc atcacgcaga gcctgaacgc ctcgcgctcg cgctgctcgt ggcgcaag
//
LOCUS       DAOSIF010000359         3284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25435_length_3284_cov_4.660266, whole genome shotgun sequence.
ACCESSION   DAOSIF010000359 DAOSIF010000000
VERSION     DAOSIF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25435_length_3284_cov_4.660266"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLT35_11010"
     CDS             <1..700
                     /locus_tag="PLT35_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG29796.1"
                     /translation="TVREYLLFVAQIKGVPSRARASRVERAMVRTWIADVKNVHCRKL
                     SKGYRQRVGLAQALLHDPDVLVLDEPTAGLDPKQIIETRQLVHELAGTHTIVLSTHIL
                     PEVAQTCQRVVIINKGKLVAVDSPEALTARLHGATPIYVQVEGGDAAADLQGVPGVTR
                     VAVADRHGSAIGYEVESTRGEDVRRDLARAVVARGLGLLELRPLRMSLEEVFLQVTTE
                     EPGGEAAPAGEATA"
     gene            697..1464
                     /locus_tag="PLT35_11015"
     CDS             697..1464
                     /locus_tag="PLT35_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG29797.1"
                     /translation="MSNVIAIARKELRAYFSSPIAYVVIGFFTLLFGYFYVAILDWFV
                     RQGMLGGMGMTASALNVNQHFVRPLLLNLTVVLLFVLPMITMRTYAEEKRAGTMELLL
                     TSPITDLQIILGKFFGALLLYAAMLAVTLVYIGVLFAVGTPEWKPIAAGYLGLLLLGA
                     SFIAVGLLISSFTRNQIVAGMLTFGVFLLLWVIDWIGTFLGPAGEKIVNYLSVVRHFD
                     DFAKGVIDTTHLVYYLSFITFGLFLTAKSVDSERWRG"
     gene            1461..3035
                     /locus_tag="PLT35_11020"
     CDS             1461..3035
                     /locus_tag="PLT35_11020"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOG29798.1"
                     /translation="MMVKRIGGIIGWVGTALVLAAVAIRFLRPEWDRVAYWSAWAGLV
                     CVLAYTLTQWRDIVNAFRRRQARLGTLTAASIAIVLGILVAINWLGTRQSKRWDLTKA
                     GQFTLADQTTNVLRKLDAPLQIRVFEKSQEMQRFKDRLAEYAGASNRVSVEYVDPDLK
                     PTLARQYQVQSYGTVVLEYKGRTERVVGDSEQDLTNGIIKVVSGEKKTIYFTQGHGER
                     DPQSSERSGYNTIKNALERENYGVSRVALAQTGAVPDDATALVVAGPTSDLLEPEVAA
                     IKAYLGKGGKLLLLVDPPESSKAAPMPGVEGLAKAWGMRLGRDIIVDASGLGQLIGSS
                     WDTPVAVSYPGHPITERFDTLTAFPLARSVSTDSPGDDGATAQPIVETGQRSWAETDL
                     DSVADKAGPKLDEAKGDRRGPIAVGAAVSRTGHAPENAAEGAKAPETRVVVMGDSDFA
                     ANMAVNVSGNRDFFMNVVGWLTQQENLIAIRPKEPGESRLTLTNDQARRIGWFGLLLL
                     PAAIFALGVHVWWRRR"
     gene            3035..>3284
                     /locus_tag="PLT35_11025"
     CDS             3035..>3284
                     /locus_tag="PLT35_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29799.1"
                     /translation="MRGLRTTLALLLVLAGLLGYIYFIELKKPPAGEAEQKEKVFSVQ
                     ADAIEHLEVKAASGDRTVLKKADGAWAIADPIAAKADEG"
BASE COUNT          525 a         1144 c         1128 g          487 t
ORIGIN      
        1 gacggtgcgc gagtacctgt tgttcgtggc gcagatcaag ggcgtgccgt cccgcgcgcg
       61 ggcctcgcgc gtcgagcgcg cgatggtccg cacgtggatc gccgacgtga agaacgtcca
      121 ctgccgcaag ctgtcgaagg gctaccgcca gcgcgtcggc ctcgcgcagg cgctgctgca
      181 cgatccggac gtcctggtgc tcgacgagcc gacggcgggc ctcgacccga agcagatcat
      241 cgagacccgg cagctcgtcc acgagctggc cggcacgcac accatcgtcc tgagcacgca
      301 catcctgccg gaagtcgcac agacctgcca gcgcgtcgtc atcatcaaca agggcaagct
      361 cgtcgcggtg gattcgccgg aggcgctgac ggcccggctc cacggcgcga cgccgatcta
      421 cgtgcaggtg gagggcggcg acgccgcggc cgatctgcag ggcgtgcccg gcgtgacgcg
      481 ggtggcggtg gccgaccgcc acggctccgc gatcgggtac gaggtggaga gcacgcgcgg
      541 cgaggacgtg cggcgcgatc tcgcccgcgc ggtcgtcgcc cgcggcctgg gcctgctcga
      601 gctgcggccg ctgcgcatga gcctcgagga ggtcttcctc caggtcacca ccgaggagcc
      661 gggcggcgag gccgccccgg cgggggaggc cacggcatga gcaacgtcat cgccatcgcc
      721 cggaaggagc tccgggcgta cttctcgtcg ccgatcgcgt acgtcgtgat cgggttcttc
      781 acgctcctct tcggctactt ctacgtcgcc atcctcgact ggttcgtccg gcagggcatg
      841 ctgggcggga tgggcatgac ggcctcggcg ctcaacgtca accagcactt cgtgcggccg
      901 ctgctgctca acctgacggt ggtgctgctc ttcgtcctgc cgatgatcac gatgcgcacg
      961 tacgccgagg agaagcgcgc cgggacgatg gagctcctgc tgacgtcgcc gatcacggat
     1021 ctccagatca tcctcggcaa gttcttcggc gcgctgctgc tctacgccgc catgctcgcc
     1081 gtcacgctcg tctacatcgg cgtgctcttc gccgtcggca cgcccgagtg gaagcccatc
     1141 gccgccggtt acctgggcct gctcctcctc ggcgcgagct tcatcgcggt cggcctgctc
     1201 atctcgagct tcacgcgcaa ccagatcgtc gccggcatgc tcaccttcgg cgtgttcctc
     1261 ctgctctggg tgatcgactg gattggcacg ttcctggggc ccgctggcga aaagatcgtc
     1321 aactacctct cggtcgtgcg ccacttcgac gatttcgcga aaggcgtcat cgacacgacg
     1381 cacctggtct actacctgag cttcatcacg ttcggcctgt tcctgaccgc caagtcggtc
     1441 gacagcgagc ggtggcgggg atgatggtca agagaatcgg cggcatcatc ggctgggtcg
     1501 ggacggccct cgtgctggcg gccgtggcga tccgcttcct caggcccgaa tgggatcgcg
     1561 tggcgtactg gagcgcgtgg gcgggcctgg tctgcgtcct ggcctacacg ctgacgcagt
     1621 ggcgcgacat cgtgaacgcc ttcaggcggc gccaggcgcg cctcggcacg ctcacggcgg
     1681 ccagcatcgc catcgtcctg ggaatcctcg tcgcgatcaa ctggctcggc acgcggcaga
     1741 gcaagcgctg ggacctgacc aaggccgggc agttcacgct ggccgaccag acgaccaacg
     1801 tcctccgcaa gctcgacgcg ccgctgcaga ttcgcgtgtt cgagaagagc caggagatgc
     1861 agcggttcaa ggaccgcctc gccgagtacg ccggggcgtc gaaccgcgtc agcgtcgagt
     1921 acgtcgaccc cgacctcaag ccgaccctcg cgcggcagta ccaggtgcag tcctacggca
     1981 cggtggtgct cgagtacaag ggacggaccg agcgcgtcgt cggcgactcc gagcaggatc
     2041 tgaccaacgg catcatcaag gtggtcagcg gcgagaagaa gacgatctac ttcacgcagg
     2101 gccacggcga acgggacccg cagagctcgg agcggagcgg ctacaacacg atcaagaacg
     2161 cgctcgagcg cgagaactac ggcgtgagcc gcgtcgcgct ggcgcagacc ggcgcggtcc
     2221 ccgacgacgc gacggcgctc gtcgtggccg gcccgacctc cgacctgctg gagcccgagg
     2281 tggccgcgat caaggcctac cttggcaagg gcggcaagct cctgctgctc gtggatccgc
     2341 ccgagtcgtc caaggccgcg cccatgcccg gcgtcgaggg cctggccaag gcgtggggca
     2401 tgcggctcgg ccgcgacatc atcgtggacg caagcggcct gggccagctc atcgggtcca
     2461 gctgggacac ccccgtcgcc gtgtcgtatc ccggccaccc gatcaccgag cgcttcgaca
     2521 cgctcacggc cttcccgctc gcgcgctcgg tgtccaccga ctcgccggga gacgacggcg
     2581 ccacggcgca gcccatcgtg gaaaccggcc agcgcagctg ggccgagacg gacctcgaca
     2641 gcgtggccga caaggccggg ccgaagctcg acgaggccaa aggcgaccga cgcggcccga
     2701 tcgcggtcgg cgcggccgtc tcgcgaaccg gacacgcccc ggagaacgcg gccgaaggcg
     2761 ccaaggcccc cgaaacccgt gtcgtcgtca tgggcgactc cgacttcgcc gccaacatgg
     2821 ccgtcaacgt gtcgggcaac cgcgacttct tcatgaacgt ggtaggctgg ctcacccagc
     2881 aggagaacct gatcgccatc cggccgaagg agccgggcga gagccgcctg acgctgacga
     2941 acgaccaggc ccggcggatc ggctggttcg ggctgctcct gctgcccgcg gcgatcttcg
     3001 cgctcggggt gcacgtctgg tggcggaggc gctgatgcga gggctccgaa cgacgctcgc
     3061 cctgctgctc gtgctcgccg gccttctcgg ctacatctac ttcatcgagc tgaagaagcc
     3121 gccggcaggc gaagccgagc agaaagagaa ggtcttctcg gtccaggcgg acgccatcga
     3181 gcacctcgag gtgaaggcgg cgtcgggcga ccgcacggtc ctcaagaagg ccgacggcgc
     3241 ctgggccatc gcggacccga ttgcggccaa ggccgacgag gggg
//
LOCUS       DAOSIF010000360         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25495_length_3279_cov_3.546526, whole genome shotgun sequence.
ACCESSION   DAOSIF010000360 DAOSIF010000000
VERSION     DAOSIF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25495_length_3279_cov_3.546526"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLT35_11030"
     CDS             complement(<1..826)
                     /locus_tag="PLT35_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG29800.1"
                     /translation="MVLTTPAVRGIRRARPDVRLSYLVEPQAAAVVARNPNLDRVIVA
                     PPARGPRRWRADLSLALALRRERYDAVIDFHGGPRSSLFALATGAPVRVGYAVPWRSW
                     MYTRLVGRPRGHRNRHSVDNQWDLAEALLPEIGRPAPDTDPVEMADDPGAASRVERRL
                     RDLGMSGGHDLVVVHVGAGNEFRRWPEEAFAGVVAALASADPNRRIILTTGAAQAGRA
                     ETVRREALRAGAAEAAVAAACDLDLGELRALAARAAVFVGGDSGPARSPSWWALPNQ"
     gene            complement(876..1064)
                     /locus_tag="PLT35_11035"
     CDS             complement(876..1064)
                     /locus_tag="PLT35_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOG29801.1"
                     /translation="MAVNPELLEILVCPACKDTPVVPVNNGAGLKCGRCHRVYPVKDD
                     IPVMLIDEASIEPPPGQP"
     gene            1153..3168
                     /locus_tag="PLT35_11040"
     CDS             1153..3168
                     /locus_tag="PLT35_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29802.1"
                     /translation="MVSLVLAAALGPAALGAPPFPRPVPQSAPAPGERPAAAARGSRP
                     ADPVAEAYFEFSRGCLLEKQGEVEGAIAAFERAMTLDPASADLPAALAELYVRQSRLA
                     DAIALAEAALKIQPAHSGAHLVLGSIYSSLAEREGPSGQERPAAADTRQLALDHLEAA
                     LGEARRDLAASVRLTLGRLYLRGGRGDKAIPVLRQLLADEPWLPQGVSMLAQAYTGAG
                     RQDDAIALLRDAAAADPSFNESLGSAYEQAERWDEAAAAFEAASKQAPDDTDLRTRWA
                     AALLNGSGRGNAGRARGLLLDVTKAHPNAGWPLYLLARAQRETGDLDGAEASARRLMA
                     VSPASASGAHALAQVLEARRDWAGIVAALEPVAAKHAQEKGPDRAMILAHLGFAYLEL
                     GRPPDAVAVFERAATLDPGEPANRLYLGQALVEAKAYDRALEVVRAERTKQPSSLRLA
                     RLEADALRGLGRFDEGAALLGALADATPQEAAPAQHLAEYYAAARRYGEAAAVLRTVV
                     RRFPDDTDALFQYGAMLERQSRRGEAERVFREVLAKDAAHAPTLNYLGYTLVDRVGRV
                     AEGLELIKRAVALDPYNGAYLDSLGWAHFKLGQLDLAEPPLKAAAAQLPRDSVVQDHW
                     GDFLFRKRRYAEAAEAWRASLAGDGEHIDRRAVERKLRGALDRLARD"
BASE COUNT          415 a         1257 c         1207 g          400 t
ORIGIN      
        1 cctgattcgg aagtgcccac caggacggcg atcgcgccgg cccgctgtcg ccgcccacga
       61 aaacggccgc ccgggccgcc agcgcccgca gttcgcccag gtccaggtcg cacgccgccg
      121 ccacggccgc ttcggcggcg cccgcgcgca gggcctcccg ccgcacggtc tcggcccgcc
      181 cggcctgcgc ggccccggtc gtgaggatga tacgccgatt cggatccgcg gacgcgaggg
      241 cggcaaccac gccggcgaag gcctcctccg gccagcggcg gaactcgttc ccggccccga
      301 cgtgcacgac gacgaggtcg tgcccgccgc tcatgccgag atcgcgcagc ctgcgctcga
      361 cgcgcgaagc ggccccggga tcgtccgcca tttcgacggg gtcggtatcg ggcgccggcc
      421 tgccgatctc aggcagcagc gcctcggcca ggtcccactg gttgtccacc gagtgcctgt
      481 tccggtggcc cctcggccgc ccgaccagcc gcgtgtacat ccagctgcgc cacggcaccg
      541 cgtatccgac cctcacggga gcgcccgtcg ccagggcgaa cagcgagctg cgcggccctc
      601 cgtggaaatc gatgacggcg tcgtaccgct cgcgccgcag cgcgagggcc agcgacaggt
      661 ccgcgcgcca cctgcgcggg ccgcgggccg gcggggcgac gatcacgcga tcgaggttcg
      721 ggtttcgcgc gaccacggcg gcggcctgcg gctcgaccag gtagctcagg cggacgtccg
      781 ggcgcgcacg gcggatcccg cgcacggccg gcgtcgtcag caccacgtcg ccgataaggc
      841 gcagccggat gagaaggatg ttcagggggc gggcctcacg gctggcccgg cggcggctcg
      901 atcgaggcct cgtcgatgag catcaccggg atgtcgtcct tcaccgggta gacgcgatgg
      961 caccggccgc acttgagccc cgcgccgttg ttcaccggga cgaccggcgt gtccttgcac
     1021 gcggggcaca cgaggatctc gagcagttcc gggttgacgg ccatcacggc gctcctttcg
     1081 cgcgctacgc cactatacca gacgcgccgc aggcaggctg acagcccggg gcctttgtga
     1141 aacaatcggg ccgtggtctc cctggttctc gcggcggccc tcggcccggc ggcgctcggc
     1201 gcgccgccgt tcccgcgccc cgtcccgcaa tcggctcctg cgcccgggga gcgccccgcc
     1261 gcggccgcgc gcggcagccg ccccgccgat cccgttgccg aggcctactt cgagttcagc
     1321 cgcggctgcc tgctcgaaaa gcagggcgag gtcgagggcg ccatcgcggc gttcgagcgc
     1381 gcgatgacgc tggatccggc ctcggcggat ctcccggccg ccctcgccga gctgtacgtg
     1441 cgccagagcc gcctggccga cgccatcgcc ctggcggagg cggcgctgaa gatccagccg
     1501 gcgcacagcg gcgcgcacct cgtgctcggg tcgatctact ccagcctcgc ggagcgcgag
     1561 ggcccgtccg ggcaggagcg tccggcggca gccgacaccc gtcagctcgc gctcgaccac
     1621 ctcgaggcgg cgctcggcga agcccggcgg gacctcgccg ccagcgtccg cctgacgctc
     1681 ggccgcctgt acctgcgcgg cggtcgcggc gacaaggcca ttcctgtgct ccggcagctg
     1741 ctggccgacg agccctggct gccgcagggc gtctcgatgc tcgcccaggc ctacaccggc
     1801 gccgggcgcc aggacgacgc cattgcgctc ctcagggacg ccgccgcggc ggacccctcg
     1861 ttcaacgaat cgctcggctc cgcgtacgag caagccgagc ggtgggacga ggccgccgcc
     1921 gccttcgaag cggcgtcgaa acaggcgccg gacgacacgg acctccggac gcgctgggcg
     1981 gcggcgctcc tcaacggctc cgggcgcggg aacgccggca gggcccgcgg cctgctgctc
     2041 gacgtgacga aggcccatcc gaatgccgga tggcctctct acctgctcgc gcgagcacaa
     2101 cgggagacgg gcgacctgga cggggcggag gccagcgcgc ggcggctgat ggccgtcagc
     2161 cccgccagcg cgtccggcgc ccatgccctg gcgcaggtcc tggaggcccg ccgcgactgg
     2221 gcgggcattg tggccgcgct cgagcccgtt gccgcgaagc acgcgcagga gaaggggccc
     2281 gatcgggcga tgatcctggc gcatctgggg ttcgcgtacc tcgaactcgg ccgccctccg
     2341 gacgcggtgg ccgtgttcga gcgagcggcg acgctcgacc cgggcgagcc cgccaaccgg
     2401 ctctacctcg ggcaggcgct cgtcgaggcg aaggcgtacg atcgcgcgct ggaggtggtg
     2461 cgggcggagc ggacgaagca gccctcgagt ctgcgcctcg cgcgcctcga ggccgacgcg
     2521 ctgcgcgggc tgggccggtt cgacgagggc gcggcgctgc tcggggccct ggccgacgcg
     2581 acgccgcagg aggcggcccc ggcgcagcac ctggcggagt actacgccgc ggcgcgccgg
     2641 tacggcgagg ccgcggccgt gttgaggacg gtcgtccggc ggtttcccga cgacacggac
     2701 gcgctgttcc aatacggggc gatgctcgag cggcagtcgc ggcgcggaga ggccgagcgc
     2761 gtcttccgcg aggtccttgc caaggacgcg gcgcacgccc cgacgctgaa ctacctcggc
     2821 tacaccctgg tcgatcgcgt cggccgcgtc gccgagggcc tggagctcat caagcgggcc
     2881 gtcgcgctcg acccgtacaa cggcgcctac ctcgacagcc tcggctgggc gcacttcaag
     2941 ctcggtcagc tcgatctcgc cgagccgccc ctgaaggccg ccgccgcgca actgccgcgc
     3001 gactcggtcg tccaggacca ctggggcgac ttcctgttcc ggaagcgccg ctacgccgag
     3061 gccgccgagg cctggcgcgc gtcgctcgcc ggcgacggcg agcacatcga tcggcgcgcc
     3121 gtcgagcgga agctgcgcgg cgcgctggac aggctcgccc gggactagcc ggtggctcga
     3181 gattcgcgca tcaccggggg tccggcgggg cccggcgccc cggccggccc gggccccgcg
     3241 ctccgcgcgt gcgtcggctg cgctctgctg gcgctggcc
//
LOCUS       DAOSIF010000361         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25529_length_3275_cov_3.214596, whole genome shotgun sequence.
ACCESSION   DAOSIF010000361 DAOSIF010000000
VERSION     DAOSIF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25529_length_3275_cov_3.214596"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PLT35_11045"
     CDS             complement(<1..1346)
                     /locus_tag="PLT35_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOG29803.1"
                     /translation="MRRGLGLVLMILLLAVLVSAAGMAVLMFVAGREPAVAADSVLEQ
                     RVEGDLADAASENVFDTLLSGRRQGVRAIVDNLRKAKTDRRIAAVVVYPRGLTSAYWA
                     KLQEVRDAIADFRASGKPAVAHLEYGGDREYFVATGCDRVFLLPTSPLDLSGLASYEL
                     FLRGTLDKIGAYPDYLHIGDYKTAPNQLTERTFTPAHREMAESLNRDLFEQLVQGVAD
                     GRNKSADEVRRIIDDGPFLAADAVQLGLVDDLAYEDEIDGKVRPGKGRVVRVKGDDYA
                     KVSPASVGLKGRSRIAVINAVGIINSGRSGIDPLNGAVLGSDTIVEYIRKARRDRSIK
                     AVVLRIDSPGGSTIASDVIWRELSLFNSGPGARPLVASMSDLAASGGYYIAVAAPDIV
                     AEPGTLTGSIGIYGGKIVAGGTYEKLGMNIESLSIGAYADMGSPVRPFSEAERAKL"
     gene            1466..2641
                     /locus_tag="PLT35_11050"
     CDS             1466..2641
                     /locus_tag="PLT35_11050"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOG29804.1"
                     /translation="MTNGTRPAPVSDKPIWETLKQDWRYVRDDFGPAWRATLRMTVAE
                     LEEFYLTPEHRSRLRGMGAVKRWFSLAWWLLKGLFLKLAPLRRVILLVALLLLLNSAQ
                     IDSVRGARVSVDGSLFAAVLLLGLLLLELKDKLLARHELEAGRAVQRALMPERCPGLG
                     GWDVWSYTRPANDVGGDLVDCLELGPGRVGFALADVAGKALPAALLMAKVQSTLRALA
                     TDVQSLSQLASKTNAILCRDGLSNRFATLVYLELRDRDGVVRLVNAGHMPPVRISGGT
                     FHELPRGDMALGMMPASTYQERQLELRPGEMLVVYSDGLTEALNEAGEFYGDERLRSV
                     FAGLGGLSAAEAGARLLASVDAFIGRTRPYDDLSLVILRREGVGTNFGSALPIATRP"
     gene            complement(2667..>3275)
                     /locus_tag="PLT35_11055"
     CDS             complement(2667..>3275)
                     /locus_tag="PLT35_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG29805.1"
                     /translation="VVVIDRGIDGDQYTTFVGADNYQIGREAGEHIAAVTGGRGRIVE
                     IRGLPGSPPAVDRSRGMHDAIAAHPDLQIIHSPVANWLREEAMSQMEMALAAHPRIDV
                     VYAHNDPMAIGAYLAAKAKGREQEMLFVGIDALPGLDGGRQAVADGRLDATFVYPTGG
                     REAVEIASRILHGEAVPHRVVLQTEKITAKGSGRISHAGPRG"
BASE COUNT          476 a         1153 c         1100 g          546 t
ORIGIN      
        1 ttcagcttcg cgcgctcggc ttcgctgaac ggccggacgg gagagcccat gtcggcgtac
       61 gcgccgatgc tgaggctctc gatgttcatc ccgagcttct cgtaggtgcc gccagccacg
      121 atcttgccgc cgtagatgcc aatcgacccc gtgagcgtgc cgggctcggc cacgatgtcc
      181 ggcgcggcga cggcgatata gtagccgccg gacgccgcca gatccgacat cgaggccacg
      241 agcggccgcg cgccggggcc ggagttgaag aggctcagct cgcgccagat gacgtctgac
      301 gcgatggtcg atccgcccgg gctgtcgatg cgcagcacga cggccttgat cgaccgatcg
      361 cggcgggcct tgcggatgta ctcgacgatc gtgtcggagc ccaggaccgc gccgttcagc
      421 ggatcgatcc cgctgcgccc cgagttgatg atgccaacgg cgttgatcac ggcaatccgc
      481 gagcgtccct tcagcccgac cgaggccggg ctcaccttcg cgtagtcatc ccccttgacg
      541 cggacgaccc gtcctttccc cgggcgcacc ttgccgtcga tctcatcctc gtaggcgaga
      601 tcgtcgacga ggccgagctg aaccgcgtcc gcggccagaa acgggccgtc gtcgatgatc
      661 cgccgcacct cgtcggcgct cttgttccgg ccgtcggcca cgccctgcac gagctgctcg
      721 aacaggtcgc ggttgagcga ttcggccatc tcgcggtgcg cgggagtgaa ggtcctctcc
      781 gtcagctggt ttggggcggt cttgtaatcg ccgatgtgga ggtagtcggg ataggcgcct
      841 atcttgtcga gggtccctcg caggaacagc tcgtacgacg ccagtccgct caggtccagc
      901 gggctcgtgg gcagcagaaa cacgcggtcg cagcccgtcg ccacgaaata ctcccggtcc
      961 ccgccgtact cgaggtgcgc caccgccggc tttcccgagg ccctgaagtc cgcaatcgcg
     1021 tccctcacct cctgcagctt cgcccagtac gcggaggtca ggccccgggg gtacacgacg
     1081 accgccgcga tgcgccggtc cgttttggcc ttgcggagat tgtcgacgat ggcccggacg
     1141 ccttgacgcc ggccgctcag cagggtgtcg aacacgttct cggaagccgc gtcggcgagg
     1201 tccccttcga cgcgttgttc gagcaccgaa tccgccgcca ccgcgggctc gcgcccggcg
     1261 acgaacatca gcaccgccat ccccgccgcc gacacgagga cggccagcag caggatcatc
     1321 agcacgagcc cgagtccgcg tcgcatcgcc accggcgtcc cctttcgctg tggcgcgagt
     1381 atagcaccgc cggtccgaac tccaggccgc cgccatccgt cctatccggt gaagggcgcg
     1441 gacatccgcg cctcgggcgc acacgatgac caacggcacc cgccccgctc ccgtgtccga
     1501 caagcccatc tgggagaccc tgaaacagga ctggcggtac gtgcgcgacg atttcggccc
     1561 ggcgtggcgg gccacgctcc ggatgaccgt ggcggaactc gaggagttct acctcacgcc
     1621 ggagcaccgc tcgcggttga ggggcatggg cgccgtcaag cggtggttct ctctggcctg
     1681 gtggctgctc aagggcctgt tcttgaaact ggcgccgctc cggcgggtca tcctgcttgt
     1741 cgcgctgctg ctcctgctca actcggccca gatcgacagc gtccgcgggg ccagagtgag
     1801 cgttgacggc tccttgttcg ccgcggtcct cctgctcggc ctcctcctgc tcgagctgaa
     1861 ggacaagctg ctggcccgac acgaactgga agcgggacgg gcggtgcagc gcgccctcat
     1921 gccggagcgc tgtcccggct tgggcggatg ggacgtctgg agctacacgc gccccgctaa
     1981 tgacgtgggc ggcgacctgg tggattgcct cgaactcggg cccggccgcg tcggattcgc
     2041 cctggccgac gtcgcgggca aggcgctgcc ggcggccctg ctgatggcga aggtccagtc
     2101 gacgctgcgg gctctcgcca cggacgtcca gtcgctgagc cagcttgctt cgaagacgaa
     2161 cgcgatcctc tgccgggacg gcctctccaa tcgcttcgcg actctcgtct acctcgaact
     2221 ccgcgaccgg gacggggtcg tgcgcctggt caacgccggc cacatgccgc cggtgcggat
     2281 ctcgggcggg acgttccacg aactgccccg cggcgacatg gcgctgggaa tgatgccggc
     2341 gtctacgtac caggagcggc agctggagct tcggccgggc gagatgctgg tggtctactc
     2401 cgacggcctg accgaggcgc tgaacgaggc gggcgagttc tacggcgacg aacgcctccg
     2461 cagcgtgttc gccggtctgg gaggactgtc ggccgcagag gccggcgcgc gcttgctggc
     2521 atcggtggat gcgttcatcg gccggacgcg tccttacgac gacctctcgc tcgtcatcct
     2581 cagaagagaa ggggtcggga cgaatttcgg atcggccctg cccatcgcga caagaccgta
     2641 atcggccgct ctcaattacg gagttgttat ccccgagggc cggcgtgtga aattcgaccc
     2701 gaccccttcg cggtgatttt ttccgtttgg agcaccaccc ggtgcggcac ggcctcgccg
     2761 tgcaggatgc gcgacgcgat ctcgaccgcc tcgcgcccgc ctgtcggata gacgaacgtc
     2821 gcgtcgagac ggccgtcggc caccgcctgc cggccgccgt cgaggcccgg cagcgcgtcg
     2881 atgcccacaa acagcatctc ctgctcgcgg cccttggcct tggcggccag gtacgcgcca
     2941 atcgccatcg ggtcgttgtg cgcgtagacg acgtcgattc gcgggtgggc cgcgagcgcc
     3001 atctccatct gcgacatggc ctcctcgcgg agccagttgg ccaccggcga gtggatgatc
     3061 tgcaggtccg ggtgggccgc gatcgcgtcg tgcatgcccc ggctccgatc gacggccggc
     3121 ggcgagccgg gcagcccgcg aatctcgacg atgcgcccgc gccctcccgt gacggcggcg
     3181 atgtgctcgc cggcctcgcg gccgatctgg tagttgtcgg cgccgacgaa cgtcgtgtac
     3241 tgatctccgt cgatcccgcg gtcgatcacg acgac
//
LOCUS       DAOSIF010000362         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25643_length_3265_cov_4.060748, whole genome shotgun sequence.
ACCESSION   DAOSIF010000362 DAOSIF010000000
VERSION     DAOSIF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25643_length_3265_cov_4.060748"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /gene="nth"
                     /locus_tag="PLT35_11060"
     CDS             <1..689
                     /gene="nth"
                     /locus_tag="PLT35_11060"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG29806.1"
                     /translation="RPDPRDVAWVLRRLGKAIAELELPVVERIAGRQDHDPFQVLIAT
                     LLSARTQDQTTGAASARLFRRAPTVQRMASLAERDIAALIRPVGFYRTKARHVKATCR
                     ALLDRHGGRVPSTMEELLALPGVGRKTANLVLILAFRSRRSICVDTHVHRISNRLGWV
                     RTKTPEATEQALYGTVPARWWPHVNRYLVTWGQRVCRPIRPRCGECLVAARCPRHGVT
                     AVGKPRAPRA"
     gene            781..1323
                     /locus_tag="PLT35_11065"
     CDS             781..1323
                     /locus_tag="PLT35_11065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG29807.1"
                     /translation="MRRPPFAAVAVAALAVWLGTQGAGFAQAPAAGPIVVIETSKGTI
                     TIETYPSEAPKTVEHFLALVKSNFYNRQHFHRAESFVIQVGDPDSKDLAKKDSWGRRG
                     HGKPIGVAEISKKLTHKKGAVGMAHAGDPTKAESQFYITRLPRKIPEGRYAIFGQVTS
                     GLDVVDKIEVGDLIKRMYVK"
     gene            complement(1420..2475)
                     /locus_tag="PLT35_11070"
     CDS             complement(1420..2475)
                     /locus_tag="PLT35_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOG29808.1"
                     /translation="MSDLRIGTSGWSYPEGPGAWNGVFYPARRTRGRSARGGDLAFYA
                     EHFATVEVNSSFYRVPDPAVTAKWAGQTPADFDFSVKLFQQFTHPAMYLKAAGRHAGE
                     EDSPIPSVTRRDVDLFKAALDPLADAGKLGALLAQFPPAFREGEDSREYLAWLLEAFS
                     GYALAVELRHRSWSDRAAEVIALLGGHGAAWVQIDEPKFRFSIRQTFAPNVPGLYYLR
                     LHGRNAAKWWTHDHPDERYDYLYSPAEISGFADAIARVRGAVRRIYVYMNNHFAGKAV
                     ANAAALRHALGQPVPGEYSDEMLERYPFLGPYATRAGAKPLLDRAAKSGSGGETGRSR
                     PPRPRRTAAGDAEPQEG"
     gene            2703..>3265
                     /locus_tag="PLT35_11075"
     CDS             2703..>3265
                     /locus_tag="PLT35_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG29809.1"
                     /translation="MLTVSEIFRSIQGESTYVGMPCVFVRLAGCDLRCAWCDTPYAFT
                     GGRRMTVDEAVAAVEGFGCRLVGVTGGEPLLQAGVYALMRRLRERGLTVLLETGGHVS
                     VERVPDGVVKILDVKCPGSGESGRMHWPNLDRLQPADEVKFVILDRADYEYARGVVER
                     HGLVSRCAAVLFSPVFGALPPADLAAW"
BASE COUNT          473 a         1135 c         1155 g          502 t
ORIGIN      
        1 cgaggcccga tccgcgcgac gtcgcctggg tcctgcgccg cctcggcaag gccatcgcgg
       61 agctggagct cccggtggtc gagcggattg ccggacggca ggaccacgat ccgttccagg
      121 tcctcatcgc gacgctcctg tccgcccgca cccaggacca gacgaccggg gccgcgtccg
      181 cccggctctt ccgccgcgcg cccaccgtcc agcgcatggc ctcgctggcg gaacgggaca
      241 tcgcggcgct catccgcccc gtcggcttct accggacgaa ggcccggcac gtgaaggcca
      301 cgtgccgggc gctgctcgac cggcacggcg ggcgcgtgcc ctcgacgatg gaggagctgc
      361 tcgcgctgcc gggagtcggg cgcaagaccg ccaacctcgt cctcatcctc gcgttcagga
      421 gccggcggag catctgcgtg gacacccacg tgcaccggat ctcgaaccgc ctcgggtggg
      481 tgcggacgaa gacgcccgag gccaccgagc aggccttgta tggcaccgtc ccggcacggt
      541 ggtggccgca cgtgaaccgg tatctcgtga cgtgggggca gcgggtctgc cgcccgatcc
      601 gcccgcgctg cggcgagtgc ctcgtggcgg cgcgctgtcc ccgccacggc gtgaccgccg
      661 tcgggaagcc gcgtgcgccg cgcgcctgat cgggttctgg tgtatcatcc ggccagctcc
      721 gatccgtccg ccggctgccc cgggcagcgg cgccggccga gggccaagag gagacccaag
      781 atgcgcagac cgccgttcgc agccgtcgcc gtggcggcgc tggccgtgtg gctcggcacg
      841 cagggagccg gcttcgcaca ggcgcccgcg gccgggccga tagtcgtgat cgaaacgtcg
      901 aaggggacca tcacgatcga aacctatccg tcggaggcgc ccaagaccgt cgagcacttc
      961 ctcgcgctcg tgaagtcgaa cttttacaac cggcagcact tccaccgggc cgagtcgttc
     1021 gtgatccagg tgggcgaccc ggactcgaag gatctcgcca agaaggactc gtggggccgg
     1081 cggggccacg ggaagccaat cggcgtcgcc gagatcagca agaagctgac gcacaagaag
     1141 ggcgccgtcg ggatggccca cgccggggat cccaccaagg cggagagcca gttctacatc
     1201 acgcgccttc ccaggaagat cccggaaggc cggtacgcga tcttcggaca ggtcacctcg
     1261 ggcctcgacg tcgtggacaa gatcgaggtg ggcgacctga ttaagcggat gtacgtgaag
     1321 tagaagtcga tcgagttcgc agggcgcgca gagccagcgg aaggcgaaga aggagcggtt
     1381 tcacgccgtg cgccgctccg gccgaccatc agcgagccat cagccttcct gcggttctgc
     1441 gtcccctgcg gccgtccgcc gcggcctcgg cggccggctc ctgcccgttt cgccgccaga
     1501 tccgctcttc gcagccctgt cgagcagcgg tttcgctccc gcgcgcgtcg cgtagggccc
     1561 caggaacggg taccgctcga gcatctcgtc cgagtactcc cctggaaccg gctggcccaa
     1621 ggcgtggcgc aaggccgccg cgttggcgac ggcttttcct gcgaagtggt tgttcatgta
     1681 cacgtagatc cggcgcaccg cgcctctgac gcgcgcgata gcgtcggcga agcccgagat
     1741 ctcggcgggc gaatagaggt agtcgtagcg ctcgtccggg tgatcgtgcg tccaccactt
     1801 cgcggcgttg cgcccgtgca gccgcaggta gtacaggccg ggcacgttcg gcgcgaacgt
     1861 ctgccggatt gagaagcgga acttcggttc gtcaatttgc acccacgccg cgccgtgccc
     1921 gccgaggagc gcgatcacct cggcggcgcg gtcgctccag ctccggtggc gcagctccac
     1981 ggcgagggcg tagccggaga acgcctcgag cagccaggcg aggtactccc ggctgtcttc
     2041 cccttcccgg aacgcgggcg ggaactgcgc gagcagggcg cccagcttgc ctgcgtccgc
     2101 cagcggatcg agggcggcct tgaagagatc cacgtcccgg cgcgtcaccg acggaatggg
     2161 gctgtcctcc tcgccggcgt gacggccggc ggccttcaga tacatggcgg gatgcgtgaa
     2221 ttgctggaac agcttcacgg agaagtcgaa gtccgccggc gtctgcccgg cccacttcgc
     2281 ggtgaccgcc gggtcgggga ccctgtagaa gctcgagttc acctcgacgg tggcgaagtg
     2341 ctccgcgtag aaggcgagat ccccgccgcg cgcgctccgg ccgcgggtcc gcctggcagg
     2401 gtagaagacc ccgttccagg cgccagggcc ctcggggtag ctccagcccg atgtgccgat
     2461 gcgcaggtcg ctcatgcccg cctccgcctc atcttacgct cgacggccgc ccgccgggac
     2521 tctggactcg gccttccgcg ccgaagccgg ccggtcggcc gtttggcggc ggcggggact
     2581 cggggcccgg gatccggtag cgagacgcac tgcccggtcc gggattctgg attctgaatt
     2641 ccggcttctc gatgctgcct cctctctccc gccttccgcc tccggtacaa tgaacccacg
     2701 ccatgttgac ggtgagcgag atcttccgct cgattcaggg tgagtccacc tacgtcggca
     2761 tgccgtgcgt gttcgtgcgg ctggcgggat gcgacctgcg ctgcgcctgg tgcgacacgc
     2821 cgtacgcctt cacgggcggc cggcgcatga cggttgacga agccgtggcg gccgtcgagg
     2881 ggttcgggtg ccgcctcgtg ggggtaaccg ggggcgaacc cctgctccag gccggcgtct
     2941 acgcgctcat gcgccgcctt cgggagcgcg gcctgacggt gctcctcgaa acgggcggtc
     3001 acgtcagcgt cgagcgggtt ccagacggcg tcgtgaagat cctcgacgtc aagtgcccgg
     3061 ggagcgggga gtcgggccgg atgcactggc cgaatctcga ccggctccag ccggcggacg
     3121 aagtgaagtt cgtgatcctg gatcgcgctg actacgagta cgcgaggggc gtggtcgaac
     3181 ggcacggcct ggtgtctcgt tgcgccgcgg tcctgttctc gccggtgttc ggcgccctcc
     3241 cgccggcgga tctcgcggcg tggat
//
LOCUS       DAOSIF010000363         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25743_length_3256_cov_3.537332, whole genome shotgun sequence.
ACCESSION   DAOSIF010000363 DAOSIF010000000
VERSION     DAOSIF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25743_length_3256_cov_3.537332"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1619
                     /locus_tag="PLT35_11080"
     CDS             219..1619
                     /locus_tag="PLT35_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG29810.1"
                     /translation="MPGFAHALPAPVLTIAHSVRAAGGRALVAGGWPRDYLLKLQPGD
                     VDLEVYGLPAPALRRLLEAVGRVDAVGEAFTVYKVAGIDVALPRRDSKVGRGHRGFEI
                     EGDPFMPVEEACRRRDFTINAMLVDPLSGEWFDPFGGRRDLEDRVLRMVDEATFGDDS
                     LRVLRALQLAARFDLRMEAGTKRVCRATCLDDLPAERVLGEFERLLLLARRPWTGFEL
                     GRELGVIEALIPEMAALVGCRQEPDWHPEGDVWTHTLMVIDEARPMIDGLSRARGLTV
                     MLGAVCHDFGKPLTTAIIDGRIRSPGHEPDGVAPALAWLDRLNLHTLDGFDVRRQVVG
                     LVAYHLAPGTWHKSASPVSDGAFRRLAQKVDLELLALVARADCRGRAGDFDCSAMDWF
                     LARARALGVEHRPPPPILLGRHLLEMGLTPGPGIGRILKAVYERQLDGEVVDLDTARR
                     AARGLLERDGAHGATQ"
     gene            1691..1996
                     /locus_tag="PLT35_11085"
     CDS             1691..1996
                     /locus_tag="PLT35_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29811.1"
                     /translation="MAADYGANGERPIPDRGADAQPPAPDTLLDHSSAVRRFDTCRWK
                     KAAEAGTPAHCTHRDVLPIAGTTGFSPEAWCGECGFFKVRRTPRKRPAPPPDDRYFY"
     gene            complement(2018..2878)
                     /locus_tag="PLT35_11090"
     CDS             complement(2018..2878)
                     /locus_tag="PLT35_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29812.1"
                     /translation="MALPESPEPSAVQRPAYRLADVLALYPSVRDDHLRTLQRWGLIG
                     APDDRAGERFIGFADVAVIRHFHAELRRGAAFRLVLRASLAARAGQLAFDFRADARPA
                     RVIALKPQPQARAEGRFVRREARRNGPCALADRAVAERYFSLASSLDDGTAEKRERAA
                     DAYRHALEADPTLVPALVNLANLHYSSGRLIEAMALYERAVALQPDAFEGCFNLGNVQ
                     HELGDYRAAAFSYVQALALDPGHADAHLYLAVVLEKDGRSEEAKPHWRSYCSLAPDGE
                     WASLARELSE"
     gene            complement(2954..>3256)
                     /locus_tag="PLT35_11095"
     CDS             complement(2954..>3256)
                     /locus_tag="PLT35_11095"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOG29813.1"
                     /translation="SVLGLVENMSYFVCPSCRHESDIFGRGGGQRLAEEMGIPFLGGI
                     PISERLREGADAGAPLVIADPSSPVAKAFASVAEQTAAQVSIQSYRSAVIPLKPVS"
BASE COUNT          456 a         1164 c         1174 g          462 t
ORIGIN      
        1 gtactttatg cgccggctcg gcgaacggcc cgcgaacgtg gcgtgggtgc tgaaggggct
       61 gattcgcgac aggggctgag cagcacgcgc tgagcgcccg tcacgcggag acagggcccg
      121 ccgcgcagag gccggtttgt ccgcccgcgg ccgacgccgt cggcccgagg ctgggccggg
      181 cggcgcctgg cgcaggctga ggcaggagac aaagagccat gcccgggttc gcgcacgcgc
      241 tccccgcgcc cgtgctgacc atcgcgcaca gcgtgcgggc agcaggcggc cgcgccctgg
      301 tggccggcgg ctggccgcgc gattacctgt tgaagctcca gcccggtgac gtcgatctcg
      361 aggtctacgg cctgcccgcc cctgcgctgc gccgcctgct cgaggcggtc ggacgggtcg
      421 acgccgtcgg cgaggcgttc acggtctaca aggtggccgg catcgacgtc gccctgccga
      481 gacgcgattc caaggtcggc cggggccacc gggggttcga gatcgagggc gatccgttca
      541 tgcccgtcga ggaggcctgc cgccggcgcg acttcaccat caacgcgatg ctcgtcgatc
      601 ccctctccgg cgagtggttc gatccgttcg gcggccggcg cgatctcgag gatcgggtcc
      661 tcaggatggt ggatgaggcc accttcggcg acgacagcct ccgggtgctc cgcgcgctcc
      721 agctcgctgc ccgcttcgac ctccggatgg aagccggcac gaagcgggtc tgccgcgcga
      781 cgtgccttga cgacctgccg gccgaacggg ttctcggcga gttcgagcga ctgctgcttc
      841 tcgcccgccg cccttggacc ggtttcgaac tggggcggga actcggcgtc atcgaggcac
      901 tcatcccgga aatggcggcc ctggtcggct gccggcagga gccagactgg catcccgagg
      961 gcgacgtctg gacgcacacg ctgatggtga tcgacgaggc ccgcccgatg atcgacggcc
     1021 tctcccgcgc ccggggcctg accgtcatgc tcggcgccgt gtgccacgac ttcggcaagc
     1081 cgctcacgac agccatcatc gacgggcgca tccggtcgcc gggtcacgag cctgacggcg
     1141 tggcgcccgc gctggcctgg ctcgaccgcc tcaacctcca cacactcgac ggcttcgacg
     1201 tgcggcgcca ggtcgtgggt ctcgtggcct accacctggc gccaggcact tggcacaagt
     1261 cggcttcccc cgtgagcgac ggggccttcc ggcgcctggc ccagaaggtc gatctggaac
     1321 tcctggcgct cgtcgcccga gcggactgcc ggggacgggc cggtgatttc gattgctcgg
     1381 ccatggactg gttcctggct cgggcgcggg ccctcggcgt ggaacatcgc cccccgccac
     1441 ccatcctgct cgggcgccat ctgctcgaaa tggggctaac gccgggccct ggcatcggcc
     1501 ggattctcaa agcggtgtac gaacgacagc tcgacggcga ggtggtcgac ctcgacacgg
     1561 cgcgcagggc ggcccgaggc ttgcttgagc gtgacggcgc acatggcgcc acgcagtagc
     1621 cgtgccagac gcggggccgc accgccgcca gaggttgcgg cgcagctacc catgtggcac
     1681 aatatttgat atggcagccg attacggcgc gaacggtgag cgtcccatcc cggatcgcgg
     1741 cgccgacgcc cagccaccgg ccccggacac cctcctcgat cactcgtctg ccgtccggcg
     1801 tttcgacacc tgccgctgga agaaggctgc cgaggccggt acgccggccc actgtacgca
     1861 ccgggacgtc ctgcccattg ccgggacgac cgggttcagc ccggaggcct ggtgcgggga
     1921 gtgcgggttt ttcaaggtcc gccgaacgcc tcgcaagcgg ccggcgccgc cgccggacga
     1981 tcgctacttc tactgaagag gcccgcttcg ccccgcgcta ctcgctgagc tcacgcgcca
     2041 ggctcgccca ctccccgtcg ggcgcgaggc tgcagtagct ccgccagtgc ggcttcgcct
     2101 cctccgaccg gccgtccttt tcgagcacca ccgcgaggta caggtgggcg tcggcgtggc
     2161 cagggtccag ggccagcgcc tggacgtacg agaacgccgc cgcgcggtag tccccgagct
     2221 cgtgctgcac attgcccagg ttgaagcacc cctcgaaggc gtccggttgg agggcgaccg
     2281 cgcgttcgta gagcgccatc gcctcgatga gccggccgct cgagtagtgg aggttggcca
     2341 ggttgacgag ggccgggacg agcgtggggt ccgcttcaag cgcgtgccgg taggcgtctg
     2401 cggcccgctc ccgcttctcg gcggtcccgt cgtccagcga ggaagcaagg gagaagtagc
     2461 gctcggccac cgcgcggtcg gcgagcgcgc agggcccgtt gcggcgcgcc tcccgtcgga
     2521 cgaagcggcc ttcggcgcgg gcctggggct gcggcttgag ggcgatcacg cgggcgggcc
     2581 tggcgtcggc gcggaagtcg aacgccagct gccccgcgcg cgccgccagc gaggcccgaa
     2641 gaaccagcct gaaggccgcg cctctccgga gctcggcgtg gaagtgtcgg atgacggcaa
     2701 cgtcggcgaa cccgatgaag cgttcgcccg cgcggtcgtc gggggcgcca atcaggcccc
     2761 agcgctggag cgtccgcagg tggtcgtcgc gaacggacgg gtacagcgcg agcacgtcag
     2821 ccagccggta ggccggccgc tggacagcgc tgggctccgg ggactccggc agtgccacgg
     2881 cggaccgttc ctccgcgcga accgcgtccg gagggccccg ccgggtcggc gcgccgacgg
     2941 cctccgcacg cagctagctc accggcttca gcggaatgac cgcggaccgg tagctctgaa
     3001 tcgacacctg cgccgccgtc tgctcggcaa ccgacgcgaa tgccttcgcg accggcgacg
     3061 acgggtcggc gatcaccagg ggcgcgccgg catcggcgcc ctcccgcagc cgctcggaga
     3121 tcgggatgcc gcccaggaag gggatgccca tctcctcggc gaggcgctgg ccgccgccgc
     3181 ggccgaagat gtcgctttcg tgccggcacg agggacagac gaagtagctc atgttctcaa
     3241 cgaggccgag cacgga
//
LOCUS       DAOSIF010000364         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_25955_length_3235_cov_3.345283, whole genome shotgun sequence.
ACCESSION   DAOSIF010000364 DAOSIF010000000
VERSION     DAOSIF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25955_length_3235_cov_3.345283"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLT35_11100"
     CDS             complement(<1..429)
                     /locus_tag="PLT35_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29814.1"
                     /translation="MTGEQVGRRLAALVACAAVAACVLPAAGAAGGGDEKRPLPPFDV
                     LAPSGEAVQSSQVGPPGQWLLVYVAPASPASIRLLEAMRAWESPGLASRAVVVIGAPA
                     AEAASFVQERGNEFPAVRWCADPDGSARRALRITGTPFILG"
     gene            complement(463..>3235)
                     /locus_tag="PLT35_11105"
     CDS             complement(463..>3235)
                     /locus_tag="PLT35_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG29815.1"
                     /translation="HYLLCDPVHPGNGVTDADGVLSFTGVIEGPVEVRAYPASPPLPS
                     NFNVCYGYASPSVTLERAFATVTAADDGGVVDMPIAAGTYTVTVSGIVTLADGVTPVP
                     KQNIDILRAADFKPAAASGSSLTTDASGQFAMTPSATVTAAGVLVRTVSPGGTGYVDT
                     IVPVSADGPVYVKAVMPALTATVSGQALTSDGVTPVAQAFSVSMHAAGGEGLGTVSSS
                     GDGQFAFATRALPTDGVRLVLRMDGLQPIEQYSGAITQNGQAVTVNFTLPPTITARIT
                     GSVVSIADHQTPIKGAFVELLVRDSAERLGRTQADADGRYSISAVLPADGLFTLVVRA
                     RYDSPNYVALEGSATSQNQTVSMPAAELPGAILKGVVRFHDGQPVPSPGIVGRDEHGR
                     EQRVWATGPDGSYLCFGWPAGAYTVTAQDTDSGLLVSGEAIIPSDSSLVTLDLTMPPS
                     GTVSVTVLDQSGAQVADAKVALVSDALWFERLAGPSEHLKPTPEGKYVFTHVPTGGLY
                     VQAARSATYASTEGVLAADDETANLTVSFQYLGSVQGEVLDNPEPTKAVGVRLTALRS
                     GGPLGRYVKEFSLPAGTTQYTVMFVPPGPVRVEAAPGLYGQGVPAGQADGVLAPGTPG
                     VLTLDLPIGNASTFGRQLTIPLAGGAFTYYVDGGGLVSASLSDGSLPNTLYRAGKLGI
                     GVNRDGMSYVPAARAELEGRQLVIGPETLGRLRPFQLTRKVYVPADGRFARWLDVVTS
                     PSDSPHTIRLTLTSPINGSSLSELVEPSDMLPGYALFSGYGNIGSSAYVFASAQPPVA
                     PVDAMYDSSPWDYPVIRAYWDVEVPAHGSIALLHFAIAAMPADADAARTLAFDLAALA
                     DPAAMEGLTEDELALIVNMRVPPPGPIAAAWPAGAQPIGARSLAGVWGGHAWLAQPAE
                     EPATVEAGK"
BASE COUNT          489 a         1158 c         1130 g          458 t
ORIGIN      
        1 gccgaggatg aacggcgtcc cggtgatgcg caaggcccgc cgcgccgatc cgtcggggtc
       61 ggcacaccag cggacggccg ggaactcgtt gccgcgctcc tgcacgaacg acgcggcctc
      121 ggcggcgggc gcgccgatga cgacgaccgc gcgcgacgcg agcccgggcg actcccaggc
      181 ccgcatcgct tcgagcagcc gaatcgacgc gggcgacgcc ggcgcaacgt agacgagcag
      241 ccactggcca ggcggcccga cctgcgagga ctggacggcc tcacccgacg gcgccagcac
      301 atcgaagggc gggagcggcc gcttctcgtc gccgcctcct gccgcgccgg cggccggcag
      361 cacgcacgcc gccacggccg cgcaggcaac cagcgctgcc aggcggcgcc cgacctgttc
      421 tccggtcatc ctgccctctc catcatgcat tcaacgcgtc gcctacttcc cggcctccac
      481 cgtggcaggt tcttcggccg gctgggcaag ccaggcgtgc ccgccccaga cgcccgccag
      541 cgagcgggcc ccgatcggct gcgcgccggc aggccacgct gccgcgatcg ggcccggcgg
      601 tggaacccgc atgttcacga tcagcgccag ctcgtcctcc gtcaatccct ccatggccgc
      661 cgggtcggcc agggcggcca ggtcgaaggc gagcgtccgc gcggcgtcag cgtcggccgg
      721 catggccgca atcgcgaagt gcaggagcgc gatcgacccg tgcgccggca cctcgacatc
      781 ccagtacgcc cggatcaccg gatagtccca cggcgacgaa tcgtacatcg cgtcgaccgg
      841 cgccacgggc ggctgcgcac tggcgaacac gtaggccgag ctgccaatgt tcccgtagcc
      901 ggagaagagc gcgtaccccg gaagcatgtc cgacggctcg acgagttcgc tgagcgacga
      961 gccgttgatg ggcgatgtca gtgtcagcct gatcgtgtgc ggcgagtccg atggactggt
     1021 gacgacgtcg agccagcgcg cgaaacggcc gtcggcgggc acgtacacct tgcgcgtcag
     1081 ctggaagggc cgcaagcggc cgagggtctc cgggccgatc acgagttgcc gcccctcaag
     1141 ctccgcgcgc gccgcgggga cataggacat cccgtcccgg ttcacgccga tgcccagctt
     1201 gccagcgcgg tagagggtgt tcggcaggga gccgtcgctg agcgacgccg acaccagccc
     1261 tccgccgtcc acatagtagg tgaacgcccc gccggccagc gggatcgtga gctgacggcc
     1321 gaacgtgctg gcgttaccga tgggcaggtc gagggtgagc acgccgggcg tgccgggcgc
     1381 gagcacgccg tcggcctggc ccgccggcac cccctggccg tacaagcctg gcgcggcctc
     1441 gacgcgcaca ggccctggcg gcacgaacat gacagtgtac tgcgtcgttc cagccggcag
     1501 cgagaattcc ttgacgtacc tgcccagcgg cccccccgag cggagtgcgg ttagacggac
     1561 gcccacggcc ttcgttggct cgggattgtc gagcacctcg ccctgaacgc tcccgaggta
     1621 ctggaatgac acggtcaggt tggcggtctc atcgtccgcc gcgagaacgc cctcggtact
     1681 cgcgtaggtc gcgctgcgcg cggcctgtac gtagagccct ccggtcggca cgtgcgtgaa
     1741 gacgtatttg ccctcgggcg tgggcttcag gtgctccgat ggcccggcga ggcgttcgaa
     1801 ccagagcgcg tcgctgacga gcgcgacctt cgcgtcggcc acctgcgcac cgctctggtc
     1861 gagcacggtg acagacacgg ttccgctcgg cggcatcgtg aggtcgagcg tcaccagcga
     1921 gctgtcggat gggataatgg cctctccgct gaccaagagg ccggagtccg tgtcctgcgc
     1981 cgtgacagtg tacgcacctg ccggccaccc gaagcagaga tagctgccgt ccggtccggt
     2041 cgcccagacg cgctgctcac ggccgtgctc gtcgcgcccc acgatgcccg gggacggaac
     2101 aggctgcccg tcatggaagc gcacgacgcc cttgaggatc gcgccgggca gttcggcggc
     2161 cggcatgctc accgtctggt tctgggacgt cgccgagccc tcgagagcga cgtagttcgg
     2221 cgagtcatac cgcgcgcgca ccaccagggt gaacagcccg tcggccggca gcacggcact
     2281 gatggagtag cggccgtcgg cgtcggcttg agtgcggccc aggcgctctg cggaatcgcg
     2341 gaccagcagt tccacgaacg cgcccttgat cggcgtctgg tggtcagcga tgctgacgac
     2401 gcttccggtg atcctcgccg tgatggtcgg cggcagcgtg aagttgacgg taaccgcttg
     2461 cccgttctgt gtgatcgcgc cgctgtactg ctcgatgggc tgcaggccgt ccattcggag
     2521 cacgagacgg acgccgtccg tcggcagcgc ccgcgtcgcg aacgcgaact ggccgtcgcc
     2581 ggaagacgac accgtcccca gcccctctcc gcctgccgca tgcatactga cgctgaaggc
     2641 ctgtgcgacc ggcgtgaccc cgtccgacgt cagcgcctgt ccgctcacgg tagccgtgag
     2701 cgccggcatg accgccttga cgtacacggg gccgtccgcg ctgaccggca cgatcgtgtc
     2761 gacgtagccg gttcctcccg gcgaaaccgt gcgcaccagc acgccggccg cggtcacggt
     2821 ggcgctcggc gtcatggcga actgcccgga cgcgtccgtc gtcaggctgc tgcccgaggc
     2881 ggctgccggc ttgaagtcgg cagcgcgcag gatgtcgatg ttctgcttcg gcactggcgt
     2941 cacgccgtcg gccaacgtca cgatgccgct caccgtcacg gtgtaggtac cggcggcaat
     3001 cggcatgtcc acgacgccgc cgtcgtcggc ggccgtgacc gtggcgaaag cccgctccag
     3061 ggtgacggac ggtgacgcgt acccgtagca cacgttgaag ttggacggca gcggcgggct
     3121 ggccgggtag gcacggacct cgacgggccc ctcgatgacg ccggtgaagc tcagcacgcc
     3181 gtcggcgtcg gtcacaccgt tccccgggtg caccgggtcg cagagcagat aatgg
//
LOCUS       DAOSIF010000365         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26078_length_3224_cov_3.883559, whole genome shotgun sequence.
ACCESSION   DAOSIF010000365 DAOSIF010000000
VERSION     DAOSIF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26078_length_3224_cov_3.883559"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2733
                     /locus_tag="PLT35_11110"
     CDS             <1..2733
                     /locus_tag="PLT35_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG29816.1"
                     /translation="DFTSILKTAPGSQAESKAGGEAQIDGASGSENRFVVDGMDTTSL
                     QTGVSGKTMLLDFIQEVQVKSSGYNAEFGGALGGVVSAITKSGTNTLRGQLGAYLQSS
                     KFYGAKRNSPGFDAYNSNIAIPSQVFPDNDWLYYSPVVDIGGPILKDRLWFYVGSAFT
                     RNQYQKDVTFYGDPKRVQRHFDWWSNAYYANYNISTQVTNNLRVKLSASNQRNSNRKT
                     APGSVAENYVFPDGTKATTQYTTATFDKLADGSINQSAFDSRWIKQGNNSSNDVYSGN
                     VDWVLTPTFFVNASGGYYRANNTTPPEFRGDSIRHIFDQANYDSWMIAQGFETVPASY
                     QQPAGYSDTISSSGTVRNIYDRAFFNGNLTYFLNFGGQHTLKAGMRFERFGNDALIGN
                     AKPNVNLVWGNTYTNQDTGQVFKGKYGYYIVTQTGTIGKVHSNNYSFWLQDSWNVNSR
                     LTINAGVRAENETVPSYKKAADAVTIKFGFKDKIAPRLGFAYDVKGDGKWKAYGSYGH
                     FFDITKLEMPRGSFGGDHWIAYYWTLDTYDFSSINCGEGTTGCPGTFISQIDYRRSSN
                     QYDAVLSKYFGRDMTGVDPNMKPVQGRELTAGIDHELNPTTSLKLRYVHKWLVRTIED
                     LGTFINGEEVYLIGNPGEAYGRIMEPLYPDFPTPKPVRKYDSIELRLTRRLSNRWAGD
                     VSYQWSRLKGNYSGLASADEGNRAAGGRTSPNVNRYFDYTIQNYDKNAKSVIGPLPTD
                     RPHVVKVQGSYDAPWGTQIGAFWSIMSGTPISTVIRYSGYPVFVNGRGDLGRSPMLSQ
                     LDLQAVQEFRLGRYRLALMANVDNVLDQKTWTNYFSPNLYGPQPYRESITLQMPPVKG
                     DNTTAAVLYQPYNPDAIAAWYRGKGATLRDNVLFKTPGYFQDRRQIRLSAKITF"
     gene            complement(2806..>3224)
                     /locus_tag="PLT35_11115"
     CDS             complement(2806..>3224)
                     /locus_tag="PLT35_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29817.1"
                     /translation="TDRAELTRLFRGVRSVLLAGAPLVIQVLNYERLARTGQRCLPLT
                     FFQDDEGEAIFLRVMTHHPGGAVTFTPSVLRYRKDGEPALEVVTSHNVPLHGWTRAEV
                     ESALEAAGFPLRQLHGTMADTPYAPTDSPDLVVVAR"
BASE COUNT          622 a         1079 c         1041 g          482 t
ORIGIN      
        1 gatttcacgt cgatcctgaa gacggcccct ggctctcagg ccgagtcgaa ggcgggcggc
       61 gaggcgcaga tcgacggcgc gtcgggctcg gagaaccggt tcgtcgttga cggcatggac
      121 acgacgagcc tgcagaccgg cgtgtcgggc aagacgatgc tcctcgactt catccaggag
      181 gtccaggtga agtcgtcggg ctacaacgcc gagttcggcg gcgcgctggg cggcgtcgtg
      241 agcgcgatca cgaagtccgg gacgaacacc ctccgcggcc agctgggcgc gtacctgcag
      301 agcagcaagt tctacggggc gaagcgcaac agcccgggct tcgacgccta caactcgaac
      361 atcgcgatcc cgtcccaggt gttccccgac aacgactggc tgtactacag ccccgtcgtt
      421 gatatcgggg gcccgatcct gaaggaccgg ctgtggttct acgtcggatc ggcgttcacg
      481 cgcaaccagt accagaagga cgtcactttc tacggcgacc cgaagcgcgt ccagcgccac
      541 ttcgactggt ggtccaacgc gtactacgcc aactacaaca tctccacgca ggtgacgaac
      601 aacctgcgcg tgaagctgtc cgcctcgaac cagcggaaca gcaaccgcaa gacggccccg
      661 gggtcggtgg ccgagaacta cgtgttcccc gacggcacga aggcgacgac gcagtacacg
      721 acggccacct tcgacaagct ggcggacggc tcgatcaacc agtcggcgtt cgacagccgc
      781 tggatcaagc agggcaacaa cagcagcaac gacgtgtact ccggcaacgt ggactgggtg
      841 ctgacgccga ccttcttcgt gaacgcgtcg ggcggctact accgggcgaa caacaccacg
      901 ccgcccgagt tccgcggtga ttcgattcgc cacatcttcg atcaggcgaa ctacgactcc
      961 tggatgatcg cgcagggctt cgagacggtt cccgcgtcgt accagcagcc ggcgggctat
     1021 tccgacacga tctcctcgtc cggcacggtc cggaacatct acgatcgggc gttcttcaac
     1081 ggcaacctga cctacttcct gaacttcggc ggtcagcaca cgctgaaggc cgggatgcgc
     1141 ttcgagcgct tcgggaacga cgccctgatc ggcaacgcga agccgaacgt caacctcgtc
     1201 tgggggaaca cgtacaccaa ccaggacacc ggccaggtct tcaagggcaa gtacggctac
     1261 tacatcgtga cgcagaccgg cacgatcggg aaggtgcact cgaacaacta ttccttctgg
     1321 ctgcaggaca gctggaacgt caactcgcgc ctgaccatca acgccggggt ccgcgccgag
     1381 aacgagacgg tcccgtcgta caagaaggcc gccgacgcgg tcacgatcaa gttcggcttc
     1441 aaggacaaga tcgcgccgcg gctcgggttc gcctacgacg tgaagggcga cggcaagtgg
     1501 aaggcctacg gcagctacgg gcatttcttc gacatcacca agctggagat gccccgcggc
     1561 tcgttcggcg gcgaccactg gatcgcgtac tactggacgc tggacaccta cgacttctcg
     1621 agcatcaact gcggcgaggg gacgacgggg tgcccgggca cgttcatcag ccagatcgac
     1681 taccgccgca gctcgaacca gtacgacgcg gtgttgtcga agtacttcgg ccgcgacatg
     1741 acgggtgtcg atccgaacat gaagccggtg cagggccgcg agctgacggc gggcatcgac
     1801 cacgagctca accccacgac ctccctcaaa ctgcggtacg tccacaagtg gttggtccgc
     1861 acgattgagg acctggggac cttcatcaac ggcgaagagg tgtacctcat cggcaacccg
     1921 ggggaggcgt acggcaggat catggagccg ctgtaccccg acttcccgac gccgaagccg
     1981 gtgcgcaagt acgattcaat cgagctgcgc ctgaccaggc ggctgtccaa ccggtgggcg
     2041 ggcgacgtca gctaccagtg gagccggctg aagggcaact acagcggcct ggcgagcgcg
     2101 gacgagggca accgggccgc gggcgggcgg acgagcccga acgtcaaccg gtacttcgac
     2161 tacacgatcc agaactacga caagaacgcc aagtcggtca tcgggccgct gccgacggac
     2221 cggccgcacg tggtgaaggt ccagggctcc tacgacgcgc cgtggggcac gcagatcggc
     2281 gccttctggt ccatcatgag cggcacgccg atcagcacgg tcatccgcta ctcggggtac
     2341 ccggtgttcg tcaacggccg cggcgacctt ggccggtcgc cgatgctgtc ccagctcgac
     2401 ctgcaggctg tccaggagtt caggctcggc cgctaccgcc tggccctgat ggccaacgtg
     2461 gacaacgtgc tggaccagaa gacgtggacg aactacttct cgcccaacct ctacgggccg
     2521 cagccgtatc gcgagagcat cacgctccag atgccgccgg tcaagggcga caacacgacg
     2581 gccgccgtgc tctaccagcc gtacaatcct gacgcgatcg cggcctggta tcgcggcaag
     2641 ggcgccacgc tgcgcgacaa cgtgctcttc aagacgcccg gctacttcca ggaccgccgc
     2701 cagatcaggc tgtcggcgaa gatcacgttc tagcctgccg tccgaacgcg caggtcaggc
     2761 cctccgggct cgccgcccgg agggcctttt tttggcacgg gcggtctagc gagccacgac
     2821 cacgaggtcc ggcgagtcgg tcggcgcgta gggcgtgtcg gccatcgtgc cgtggagctg
     2881 gcggaggggg aaccccgccg cctcgagcgc cgactccacc tcggcccgcg tccagccgtg
     2941 cagcggcacg ttgtggctgg tgacgacctc gagcgcgggc tccccgtcct tccggtagcg
     3001 cagcaccgac ggcgtgaacg tgacggcgcc gccggggtgg tgcgtcatca cgcgcaggaa
     3061 gatcgcctcg ccctcgtcgt cctggaagaa ggtcagcggc aggcagcgct ggcccgtcct
     3121 ggccaggcgc tcgtagttca gcacctggat cacaagcggc gccccggcga ggagcaccga
     3181 ccgcacccct ctgaagagcc gcgtcagttc ggcccggtcg gtga
//
LOCUS       DAOSIF010000366         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26231_length_3209_cov_2.970831, whole genome shotgun sequence.
ACCESSION   DAOSIF010000366 DAOSIF010000000
VERSION     DAOSIF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26231_length_3209_cov_2.970831"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1646)
                     /locus_tag="PLT35_11120"
     CDS             complement(129..1646)
                     /locus_tag="PLT35_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOG29818.1"
                     /translation="MSSRKTTVFYAGLIAAASLAVGMVLASRLGLSPRSEAVTLTPQV
                     GVSAPITGAIDAQTFREIAKAMTPTVVNIRTIAKRRTQDMTDFFGQDDMLRRFFGLPQ
                     QGERKPRDEVTQAAGTGFIISKDGYILTNNHVVEGATKIEVAFFGDEDGEAYPATIVG
                     RDQLTDSALLKLTTMPPHPLQEARLGDSTQIAPGDWVMAIGNPFGLSHTISVGVVSAI
                     GRPMQMADQRWEEVIQTDAAINPGNSGGPLLNLRGEVIGVNTAIYTNGQAPANIGIGF
                     AVPIKAVRDVLPQLETGKVVRGRIGLQVRDVPRDALGEFGLKARQGALVSVVTPGGPS
                     AKAGIEPGDVIVQFNGKPVANRTVLVQLVMATKPGTTVPVRVIRDKAEKSLSVTVEEL
                     DLESEGGGRPSSPPEEAVGSGFGITLGPLGSEMARRLEVPSGTRGALITDVERTSTAY
                     REGLRAGDVILKINGQAVETVSEASRALRAIRSGGAARMLLWKSGQETFVIVTKE"
     gene            1878..3179
                     /locus_tag="PLT35_11125"
     CDS             1878..3179
                     /locus_tag="PLT35_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG29819.1"
                     /translation="MYKSLAGTLALILTTAALAGQAPAPVAPPRPDAQVPPITFRAEV
                     DFVEVDAVVRDAQGGFVRDLRQEEFQVFEDGVPQTVTAFSLIDIPIVPAERAMFAAQP
                     IEPDVRSNEGSGGGRLYVLLLDDLHTAFQRSIRVREAAKQFISRNLGSNDLVAVVHTS
                     GRTDAAQDFTNSRRLLTQAVDKFMGRKLRSAFLNRLDVYNRNRVTGSTTTIADAEEAE
                     RLHHARSLLATVKNLSEWLGGIHGRRKAVILMGEGLDYGILGSVISSELTETSTSSST
                     DGGLGTPPQTRVRPNRGDGYTLLTETREAIDAATRSNVNIYAIDPRGLAVPDEERITE
                     AGVLPDDPFLGIRPTDLADELRTSQDSLRVLAGETGGFASISSNDFAPALDRIVAENS
                     SYYMLGYHPTNDKRDGRFRRIEVKLTRPGLDVRARRGYGAP"
BASE COUNT          467 a         1155 c         1107 g          480 t
ORIGIN      
        1 agtagccggc ccccggcgcg tagagcgcca ggtccatgaa ctcggccgcc gtgatcggac
       61 cccgctgcct gatgcggccg gcgaccagcg cgccgcagcc ggagtccgtc atcgcccggc
      121 cgccgccgtc attccttcgt cacgatgacg aaggtctcct gcccggactt ccacaggagc
      181 atccgcgccg cgccgccgct ccggatcgcc ctgagcgccc gcgaggcttc cgagacggtc
      241 tccacggcct ggccgttgat cttcaggatc acgtcgcccg cgcgcagccc ctcgcggtag
      301 gccgtcgagg tccgttccac gtcggtgatg agcgcgcccc gcgttcccga cgggacctcg
      361 aggcggcgcg ccatctcgga gccgagcggg ccgagggtga tgccgaagcc gcttccgacg
      421 gcttcttcag gcggactgct gggccggccg ccgccctcgc tttcgagatc gagctcctcg
      481 acggtgaccg acaggctctt ctcggccttg tcgcggatga cgcgcacggg gaccgtcgtg
      541 ccgggcttgg tggccatcac cagctggacc agcacggtgc ggttcgccac cggcttgccg
      601 ttgaactgca cgatgacgtc gcccggctcg atgccggcct tcgcgctcgg gccgccaggc
      661 gtcaccaccg acacgagggc gccctggcgc gctttcagcc cgaactcgcc gagcgcgtcg
      721 cgcggcacgt cgcgcacctg gagcccgatg cgcccgcgga ccaccttgcc cgtctcgagc
      781 tggggcagga cgtcgcggac cgccttgatg gggaccgcga agccgatgcc gatgttcgcc
      841 ggcgcctggc cgttcgtgta gatggccgtg ttgacgccga tgacctcgcc gcgcaggttg
      901 aggagcggcc cgccggagtt gccgggattg atcgccgcgt cggtctggat gacctcttcc
      961 cagcgctgat cggccatctg catcggccgc ccgatcgcgc tgacgacgcc gacgctgatc
     1021 gtgtggctga ggccgaaggg gttgccaatc gccatgaccc agtcgcccgg ggcgatctgc
     1081 gtcgagtcgc cgagcctggc ttcctggagc ggatgcggcg gcatcgtcgt cagcttcagc
     1141 agggcgctgt cggtgagctg atcgcgcccg acgatggtgg ccgggtaggc ctccccgtcc
     1201 tcgtccccga agaacgcgac ctcgatcttg gtcgcgcctt ccacgacgtg gttgttcgtg
     1261 aggatgtaac cgtctttgct gatgatgaaa cccgtgccgg cggcctgcgt cacttcgtcc
     1321 cggggcttcc gttcaccctg ctggggcagg ccgaaaaagc gccgcagcat gtcgtcctgg
     1381 ccgaagaagt ccgtcatgtc ctgggtccgg cgcttcgcga tcgtccggat gttcacgacg
     1441 gtcggcgtca tcgccttggc gatctcgcga aacgtctggg cgtcgatggc cccggtgatc
     1501 ggcgcgctga cgccgacctg gggcgtgagc gtgaccgcct cggaccgagg ggagagaccg
     1561 agccgcgagg cgagcaccat ccccacggcc aacgacgccg ccgcgatgag ccccgcatag
     1621 aaaaccgtgg tcttgcgact ggacattgcg tgcctccacc gcactcctgc ccgctagtat
     1681 acccatccac cgcgggcctt cttcttcttt gacgtgcggc cgggccggcc ggttttcggc
     1741 cgccgggggc ccgcccgcgc gcagccggcg ggcagcggga ggccgacggc cggggagggg
     1801 ccccggacgt cctataatcg agcacagggg gggcagccgg cccaatcggc ccggcgtcgc
     1861 tcccgcgggg acgcgtcgtg tacaaatcgc tcgccgggac tctcgcgctc atcctgacca
     1921 cggccgcgct tgcgggccag gccccggcgc cggtggcgcc gccgcgcccg gatgcccagg
     1981 ttccgccaat cacgttccgc gccgaggtgg acttcgtcga ggtcgacgcg gtggtccgcg
     2041 acgcgcaggg cggcttcgtg cgcgacctga ggcaggagga gttccaggtc ttcgaagacg
     2101 gcgtgccgca gacggtcacg gcgttctcgc tcatcgacat tcccatcgtc ccggcggagc
     2161 gggcgatgtt cgcggcgcag ccgatcgaac cggacgtccg ctcgaacgag gggagcggcg
     2221 gcggccgcct gtacgtgctc ctgctggacg acctgcacac ggcgttccag cggtccatcc
     2281 gcgtgaggga ggccgcgaag cagttcatca gccgcaacct cgggtccaac gacctcgtgg
     2341 ccgtcgtcca caccagcggc cgcaccgacg ccgcccagga cttcaccaac agccggcggc
     2401 tgctgacgca ggccgtggac aagttcatgg gccggaagct ccgctcggcg ttcctgaacc
     2461 ggctggacgt gtacaaccgg aaccgcgtga ccggcagcac gacgacgatt gccgacgccg
     2521 aggaggccga gcggctgcac cacgcacgga gcctgcttgc gacggtgaag aacctgtccg
     2581 agtggctggg agggatccac ggccgccgga aggccgtcat cctcatgggc gaggggctcg
     2641 actacggcat cctcggctcg gtgatctcgt ccgagctgac agagacctcg accagcagct
     2701 cgaccgacgg cggcctcggg acgccgccgc agacgcgcgt gcggccgaac cgcggcgacg
     2761 gctacacgct gctgaccgaa acccgggagg ccatcgacgc ggcgacccgc tccaacgtca
     2821 atatctacgc gatcgatccg cgcgggctcg ccgtgcccga cgaggagcgg atcacggagg
     2881 cgggcgtcct gcccgacgat ccgttcctcg gcatccggcc caccgatctg gccgacgagc
     2941 tccggacgtc gcaggacagc ctccgcgtgc tggccggcga aacgggcggc ttcgcgtcga
     3001 tctccagcaa cgacttcgcg ccggcgctgg atcgcatcgt cgccgagaac agctcctact
     3061 acatgctcgg ctaccacccg accaacgaca agcgcgacgg gcggttccgc cgcatcgagg
     3121 tgaagctgac caggccgggc ctggacgtcc gggcgcggcg ggggtacggc gcgccgtagc
     3181 cgcggagcaa aatcactagc gctaaccat
//
LOCUS       DAOSIF010000367         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26252_length_3207_cov_3.334708, whole genome shotgun sequence.
ACCESSION   DAOSIF010000367 DAOSIF010000000
VERSION     DAOSIF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26252_length_3207_cov_3.334708"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLT35_11130"
     CDS             complement(<1..632)
                     /locus_tag="PLT35_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOG29820.1"
                     /translation="MFKKVLIANRGEIALRIILACRELGIRTVAVYSEADEHALHVRF
                     ADEDVCIGPPRAADSYLNVPAIISAAEVTGADAIHPGYGFLAESAYLAEVCEACHVRF
                     IGPSPQVIRLMGDKARARRAMKKAGLPVLPGSDGALESEERALRAAHEIGYPVLIKAT
                     AGGGGRGMRVVRTPSEMGAAFRTASREAEAAFGVPDVYVEKYLENPRHVE"
     gene            complement(632..1108)
                     /gene="accB"
                     /locus_tag="PLT35_11135"
     CDS             complement(632..1108)
                     /gene="accB"
                     /locus_tag="PLT35_11135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOG29821.1"
                     /translation="MDEIRGVLDLVKAYDLQELEIEADGLKIRVRTAGLVHPAHHAAP
                     AAAGAGLAAEAGASAPRAVAVGRADRPADATDAADLAIVTSPIVGTFYRAPEPGAPPF
                     VEAGTRVKRGQVLCIIEAMKLMNEIESDHDGELVAVYVENGSPVQYGERLFAIKLS"
     gene            complement(1119..2279)
                     /locus_tag="PLT35_11140"
     CDS             complement(1119..2279)
                     /locus_tag="PLT35_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOG29822.1"
                     /translation="MPHPAVDLLAARHAAVRAAAAMEGLDGLVVFHLPNIRYLTGFAG
                     SAAIAILTRDEIILITDGRYVTEVEETVRPGCPGLRLVKVDPAYDATLAACLGAAGAI
                     RVGFESAHLSVKRHDALAAALRNASAGGGSAAALVPAERLVEACRMRKDAAELAALRR
                     GGALIDAIAAAVLGDVRAGQPEWEVAAGIDDRIRRGGFERTSFETIVASGPNAALPHA
                     RPGRRVLQPGDLVVLDFGGVCEGYCTDITRTVSVGEPSAEARGLYEAVAAAHRAAAAA
                     VRPGAPITDVDEAARAVLEARGFGTAFSHATGHGLGLEVHEEPRVGPRRPDIPGVPPA
                     GRDDRLEPGIVFTIEPGAYVPGLGGVRIEDDVLVTPDGLEWLTGAPRGLRVV"
     gene            complement(2316..2918)
                     /locus_tag="PLT35_11145"
     CDS             complement(2316..2918)
                     /locus_tag="PLT35_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG29823.1"
                     /translation="MAEDPRIDALKKRIQRDPGSIAFAQLAEEYRRAGRFQESIEVCR
                     AGLARHPGYLSARVTLGRALLEVGDIDSAHREFSDVIRVAPGNLSAIRGLAEVHRRRG
                     ELPEAIEQFRSAFELAGPDPAIEQIVRDLRREAMQAPLPAEPGRASESAPGSYAAGHA
                     PTRPAASEEQRAARRTVAYLERWLEAIVADRRSRLFASRA"
BASE COUNT          433 a         1210 c         1131 g          433 t
ORIGIN      
        1 aactccacgt ggcgcgggtt ctcgaggtac ttctcgacgt acacgtccgg cacgccgaag
       61 gccgcctccg cctcgcgcga ggcggtccgg aacgcggcgc ccatctccga tggcgtccgc
      121 acgacccgca tgccgcgccc gcccccgccc gccgtcgcct tgatgagcac cgggtagccg
      181 atctcgtgcg ccgcccgcag cgcccgctcc tcggactcga gcgcgccgtc cgagccgggc
      241 agcacgggca ggccggcctt cttcatcgcc cttcgcgccc gtgccttgtc ccccatcagg
      301 cggatgacct gcggactggg gccgatgaac ctgacgtggc acgcctcgca gacctccgcc
      361 agatacgcgc tctcggccag gaagccgtac ccagggtgga tcgcgtccgc gcccgtcacc
      421 tccgcggcgc tgatgatcgc cggcacgttc aggtagctgt cggcagcccg aggcgggccg
      481 atgcacacgt cctcgtcggc gaaccgcacg tgcagggcgt gctcgtcggc ctcggagtag
      541 accgccacgg tgcggattcc gagttcccgg cacgcgagga tgatccgcag ggcgatctcg
      601 ccgcggttgg cgatgaggac cttcttgaac atcagctgag cttgatggcg aagaggcgct
      661 cgccgtactg cacggggctg ccgttctcca catagacggc cacgagttca ccgtcgtggt
      721 ccgattcgat ctcgttcatg agcttcatcg cctcgatgat gcagagaacc tggccccgct
      781 tcacccgcgt ccccgcctcg acaaacggcg gcgcccctgg ctccggcgcg cggtagaagg
      841 tgccgacaat cggcgacgtg acgatggcga gatccgccgc gtcggttgcg tcggccggcc
      901 ggtccgcccg tccgacggcc acggcccggg gggccgaggc tccggcctcc gccgccaatc
      961 cggcgccggc cgccgcgggc gcggcatgat gggccgggtg gacgaggccg gccgtcctga
     1021 cgcggatctt cagcccatcg gcctcgatct ccagttcctg gaggtcgtac gccttcacca
     1081 ggtcgagcac gccgcggatc tcgtccatgt tcatcgcgtt acaccacgcg gagtccccgc
     1141 ggcgccccgg tcagccattc cagcccgtcg ggcgtgacca gcacgtcgtc ctcgatccgc
     1201 acgccgccga gccccggcac gtaggcgcct ggctcgatgg tgaagacaat gccgggctcg
     1261 aggcggtcgt cgcggcccgc cggcggcacg cccgggatgt ccggccgccg aggcccgact
     1321 cgcggctcct cgtggacctc caggcccagc ccgtgcccgg tggcgtggct gaacgccgtc
     1381 ccgaagccgc gcgcctcgag gaccgcccgc gccgcctcgt ccacgtccgt gatcggcgcg
     1441 ccgggccgca ccgccgcggc cgccgcgcgg tgcgcggccg ccacggcctc gtagagcccc
     1501 cgcgcctcgg ccgagggctc gccaacgctc acggttcgag tgatatccgt acagtatcct
     1561 tcgcaaacgc ctccgaagtc cagcacgacc aggtcgccgg gctgaaggac ccgcctgccc
     1621 ggcctggcgt gcggcagcgc agcattgggg ccggacgcca caatcgtctc gaacgacgtg
     1681 cgctcgaacc cgcccctgcg gatccggtcg tcgatgccgg cggccacctc ccattcgggc
     1741 tggcccgccc ggacatcgcc gagcaccgcg gccgcgatcg cgtcgatgag cgctcccccg
     1801 cgccggagag cggcgagttc ggccgcgtct ttgcgcatcc ggcaggcttc gaccaggcgc
     1861 tcggcgggca cgagggcggc ggcgcttccg ccgccagccg acgcgttccg cagcgccgcg
     1921 gcgagggcgt cgtggcgctt gacgctcagg tgcgccgact cgaacccgac ccggatcgca
     1981 ccggctgccc ccaggcacgc ggccagcgtg gcgtcgtacg ccgggtccac cttcacgagg
     2041 cgcagcccgg ggcagccggg ccggaccgtc tcctcaacct cggtgacgta gcggccgtcc
     2101 gtgatgagga tgatctcgtc gcgggtgaga atcgcgatgg ccgcactgcc cgcgaagccg
     2161 gtcaagtagc ggatgttggg caggtggaag acgacgaggc catcgaggcc ttccatggcc
     2221 gcggcagcgc ggacggccgc gtgtctggcg gccaggaggt caacggctgg atgcggcatg
     2281 gatgagaggg agaatccccc acgcgccggg tcctgtcaag cgcgggaggc gaacaggcgc
     2341 gagcgccggt cggccacgat ggcctcgagc cagcgctcga ggtaggcgac cgtcctccgg
     2401 gctgcgcgct gctcctcgct ggcggcgggg cgcgtgggcg cgtgacccgc cgcgtacgag
     2461 cccggagcgc tctccgacgc ccgaccgggt tcggccggca ggggcgcctg catggcctcc
     2521 cggcggaggt cccggacgat ctgctcgatg gccgggtcgg gcccggcaag ctcgaacgcc
     2581 gagcggaact gctcgatggc ctcgggcagt tcgccgcgcc tccggtggac ctccgcaagg
     2641 ccgcggatgg cggacagatt gccaggcgcc acacggatca cgtcggagaa ctcgcgatgt
     2701 gcggaatcga tgtcgccgac ctcgagcagc gcgcggccga gcgtcacccg ggccgacagg
     2761 tagcccgggt gccgcgccag gccggcccgg cagacctcaa tggactcctg gaaccgcccg
     2821 gcccggcggt actcctccgc cagctgggcg aaggcaatcg accctgggtc cctctggatc
     2881 cgcttcttca gcgcatcgat gcgcgggtcc tcggccacga gctgatccta ctaccagcgg
     2941 ggacggcgcg tgaagggccg cagggctcgt cccgggccgg gtgaagaccg gcggcggcgc
     3001 gtgaacgccg ccgccggcag gggaatcccc cggcgccgcg cgcccgctcg aggcgagctc
     3061 gccagcctac aagccgcagc ccgtgaccga gacgttcttc gtcacgctgg cgctcgcgcc
     3121 cgtgctgtcc ctgacggtca gctgcacggc gaacggcacc gtcgccggtc cgcccgggca
     3181 gttgaaccgg tggctgggcg acaggcc
//
LOCUS       DAOSIF010000368         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26400_length_3192_cov_3.165763, whole genome shotgun sequence.
ACCESSION   DAOSIF010000368 DAOSIF010000000
VERSION     DAOSIF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26400_length_3192_cov_3.165763"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="PLT35_11150"
     CDS             <1..1265
                     /locus_tag="PLT35_11150"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOG29824.1"
                     /translation="ATGADVIGLNCSVGPAPLLESIERMAGVTRARLSAQPNAGKPRD
                     IEGRTIYLSSPEYMASYARRFVQRGVRLVGGCCGTTPAHIRQVKAAVRAISPGHGRVS
                     AAAIAPAPAGVRPATAAAPVPREHKSRLAHAIARGAFVRIVEQEPPRGHDCGPLLERA
                     RVLRIRGIDAISVPDSPRVRASTSALATAVLVEQRAGIETLLHYTCRDRNLLGMQSDL
                     LGAHAMGLRNLLVTTGDPPMEGDYPQATGVFDVDSIGLANVVSRLNHGLDVGGASLGE
                     PTSFHLGVAVNPTAIDLDLEARRFAFKAEAGAELAVTQFVFDVDALRAFLSRMGPSRV
                     PVIAGIWVFDSLRNAEYMANEVPGIRVPPPLIDRMKRAERKGRAAAEGAAIARELVAA
                     VQPVVSGVLIGTAGHDFASLLSVLEATA"
     gene            1262..1867
                     /locus_tag="PLT35_11155"
     CDS             1262..1867
                     /locus_tag="PLT35_11155"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG29825.1"
                     /translation="MSPIFDPQSPLFDHLVFLGALVRNPRSVGAIVPSSERVAADMVT
                     SVQPASRVVELGPGTGVITKAVLARLGPSGALLAVETNHVFVERLQRSWPKADVVCAS
                     AEALPDLTASRGWEGVDHIVSGLPFATLPRPVTLHILDAIRDVLKVGGTFTTFQYAHA
                     YRLPPAVRFRRELSDRLGSPPRSHLVLWNVPPAHILTWVRR"
     gene            complement(2008..>3192)
                     /locus_tag="PLT35_11160"
     CDS             complement(2008..>3192)
                     /locus_tag="PLT35_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29826.1"
                     /translation="GAAWPPRQLRPSIRPAVSALPLPRRAGPVLVFYISGHAFGHASR
                     VIEVINEVLALDPGARIVVRTGAARWLFDVTVRGPFEFHACPCDTGVVQRDSLHLDIP
                     ATIARARAFMSGLDGLARAEGAFLRAAGATLVVGDIPPLAFAAAREAGLPAVALGNFT
                     WDWIYGAYTDEIAAASELLPAIRRAYRGATATLRLPLWGGFEDWESPITDLPFIARHS
                     RREAADVRQILGLPDGTRVVLTSFGGLGIAGLSLATLSRLDGYTVIATGYGLGPQAPV
                     PPNVRLLDDRGLYERGLRYEDLVRASDVVVTKPGYGIIAECLANGAAMLYTSRGRFAE
                     YDVLVAGLPAVLRSRFIGHGDLFGGRWQAHLDAVLAQAAPPEKPRTDGAVLAATFLLG
                     LA"
BASE COUNT          456 a         1125 c         1189 g          422 t
ORIGIN      
        1 aagccaccgg ggccgacgtc atcggcctca actgcagcgt tggcccggcg ccgctgctcg
       61 agtcgatcga gcggatggcg ggagtgacgc gtgcgcggct gtcggcgcag cccaacgccg
      121 gcaagccccg cgacatcgag ggccgcacga tctacctcag ctcgccggaa tacatggcct
      181 cgtacgccag gcggttcgtc caacggggcg tgcgcctggt cggcggctgc tgcggcacga
      241 cgcccgcgca catccgccag gtgaaagccg ccgtgcgggc aatctcgccg ggccacgggc
      301 gggtgtcggc ggcggcgatc gcgccggcgc ctgcgggggt gcgccccgcc acggctgccg
      361 cgccggtgcc acgcgagcac aagtcgcgcc tcgcgcatgc gatcgcgcgg ggggccttcg
      421 tgaggatcgt ggagcaggag ccgccgaggg ggcacgactg cgggccgctc ctcgagcgtg
      481 cgcgcgtgct ccggatcagg ggcatcgacg cgatcagcgt gccggattcg ccgcgcgtgc
      541 gcgcctcgac gagcgcgctg gccacggccg tcctcgtcga gcagcgggca ggcatcgaga
      601 cgctcctgca ctacacctgc cgggaccgca acctgctcgg catgcagtcc gacctgctcg
      661 gcgcccacgc catgggactc cggaacctgc tcgtcacgac gggcgacccc ccgatggaag
      721 gggactaccc ccaggcaacc ggcgtcttcg acgtcgactc gatcgggctg gccaacgtcg
      781 tgagccggct gaaccacggg ctcgacgtcg gcggcgcgtc gctcggtgag cccacgagct
      841 tccacctggg cgttgccgtg aacccgacgg cgattgacct cgacctggag gcgcggcgct
      901 tcgcgttcaa ggccgaggca ggggccgaac tcgcggtcac ccagttcgtg ttcgacgtgg
      961 acgcgctgcg cgcgttcctc tcgaggatgg ggccctcccg tgtgcccgtc atcgccggca
     1021 tctgggtgtt cgactcgctg cggaacgccg agtacatggc caacgaagtg ccgggcatcc
     1081 gggtgccgcc gccattgatc gaccggatga agcgggcgga gcgcaagggg cgggccgcgg
     1141 cagaaggggc ggccatcgcg cgggaactcg tcgccgccgt gcagccggtg gtgagcggcg
     1201 tgctgattgg aaccgccgga cacgatttcg cgtctctact cagcgtgctc gaggccactg
     1261 cgtgagcccc atcttcgatc ctcagtcccc gctcttcgat cacctcgtct tcctcggggc
     1321 gctcgtccgc aatcccaggt cggtcggggc gatcgtgcca agctccgagc gcgtggcggc
     1381 ggacatggtg acgagcgtgc agccggcgtc gcgggtggtg gagctcggtc cgggcacggg
     1441 cgtcatcaca aaggcggtgc tcgcgaggct cgggccctcc ggcgcgctgc tggccgtcga
     1501 gactaaccac gtcttcgtcg agcggctgca gcggagctgg cccaaggccg atgtcgtctg
     1561 cgcgtcggcg gaggcgctgc cggacctgac cgcgtcgcgc ggatgggagg gcgtcgatca
     1621 catcgtgtcg gggctgccct tcgccacgct gccccggccg gtgacgctgc acatcctcga
     1681 cgccatccgc gacgtgctga aggtcggcgg gacgttcacc acgttccagt acgctcacgc
     1741 gtaccggctg ccgccggccg tcaggttccg ccgcgagctg agcgatcggc tcggttcgcc
     1801 tccgcgctcg catctcgtcc tctggaacgt gcctcctgcg cacatcctta cctgggtccg
     1861 ccgctagccc ggcgccaggg ccttccgccc gttgaggacg gggccggccc ctgcaccgtc
     1921 tgactgggtc atcaggagag ggcgaaccgg tgggatcctt ggaccgcccg agggcgcgag
     1981 cgcagccaac gccggcccgc ccgctcgtca ggcgaggccg aggaggaacg tggcggcgag
     2041 gacggcgccg tcggtgcgag gcttctcggg cggggcggcc tgcgccagca cggcatcgag
     2101 gtgcgcctgc caccggccgc cgaacagatc gccgtggccg atgaaccggc tgcgcaggac
     2161 ggccggcagg ccggcgacca ggacgtcgta ctcggcgaag cggccgcgcg acgtgtagag
     2221 catggcggcg ccgttggcca ggcactctgc gatgatgccg tatcccggtt tcgtcaccac
     2281 cacatcggac gcgcgcacga ggtcctcgta gcgcaggccc cgctcgtaga ggccgcgatc
     2341 gtccagcagg cggacgttcg gaggcacggg ggcctgcggc ccgaggccgt agcccgtggc
     2401 gatcacggtg tagccgtcga ggcggctcag cgtggcgagc gagaggcccg cgatgccgag
     2461 cccgccaaac gaggtcagca ccacgcgcgt gccgtcggga agccccagga tctgccggac
     2521 gtcggcggct tcgcgccgcg agtggcgggc gatgaacggc aggtcggtga tgggcgactc
     2581 ccagtcctcg aagccgcccc acagcggcag ccgcagcgtc gcggtggcgc cccggtaggc
     2641 ccgccggatg gccggcagaa gctcggacgc cgcggcgatc tcgtcggtgt acgcgccgta
     2701 gatccagtcc caggtgaagt tgccaagggc caccgcgggc aggccggcct cgcgcgcggc
     2761 ggcgaatgca agcgggggaa tgtcgccaac gacgagcgtc gcgccggccg cccggagaaa
     2821 cgctccctcg gcgcgagcca ggccgtccag gcccgacatg aacgcccgcg cgcgcgcaat
     2881 cgtcgcgggg atgtccaggt gcaggctgtc gcgctggacg acgccggtgt cgcacgggca
     2941 ggcgtggaac tcgaacggcc ccctgacggt gacatcgaac agccacctgg ccgcgccggt
     3001 gcgcacgacg atccgcgccc cgggatcgag cgcgagcacc tcattgatga cctcgatgac
     3061 gcgcgaggcg tggccgaagg cgtggcccga gatgtagaac accagcacgg gaccggcgcg
     3121 acgcggcaag gggagggcgc tcacggcagg ccggatcgag gggcgcagct gacgcggggg
     3181 ccaggcagct cc
//
LOCUS       DAOSIF010000369         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26426_length_3189_cov_3.158264, whole genome shotgun sequence.
ACCESSION   DAOSIF010000369 DAOSIF010000000
VERSION     DAOSIF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26426_length_3189_cov_3.158264"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1034
                     /locus_tag="PLT35_11165"
     CDS             138..1034
                     /locus_tag="PLT35_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOG29827.1"
                     /translation="MSVLTAQPVSQFVAKDYKSDLKPIWCPGCGDFGVVQAIYRALAN
                     IGRAPHEIAFVSGIGCSSRIPGYTTAYGFNSVHGRALPIAQGIKLANPDLLVLCAGGD
                     GDGFSIGGGHVAHVVRRNIDLTYIVMDNQIYGLTKGQLSPTSRRGLVTASSALGSMEG
                     PVNPLMYVLGYGASFVAQGTPADMAGLASIIEEAIRFPGFAFVNVQSPCVTYGQEDQL
                     LKAQKAMMQPLASLNHDPANRLAALDLAQAYGEKLYTGVFYRNPDPPPTFEALVRQRQ
                     EQQRPLARSRADVLNVFRLKTP"
     gene            complement(1041..1448)
                     /locus_tag="PLT35_11170"
     CDS             complement(1041..1448)
                     /locus_tag="PLT35_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29828.1"
                     /translation="MRVLTALLFPQPRRRLRHGRALNIAARTVHLAATGTLLGGHVVA
                     ADPGRLVPLVWVAVATGAVMMAVEIYPTAHWAHQVCALFVYAKLLLLGLVPFLWEHRV
                     ALLLAAVALASVGSHAPRRIRHYSVWYRRVMAE"
     gene            1517..1990
                     /locus_tag="PLT35_11175"
     CDS             1517..1990
                     /locus_tag="PLT35_11175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOG29829.1"
                     /translation="MTALSIRQARSGDAVWAAALMASSDPWITLGRGFEACHQACTWA
                     GDVLEIAELEGERCGFVLVRPSGVAGAPYVKSIAVAPGFRSRGVGAALLGHVERTYAG
                     RARHLFLCVSSFNDRARLFYERQGYAAVGAIKDFLIEGADEILMQKRLPAPDAAA"
     gene            2068..>3189
                     /locus_tag="PLT35_11180"
     CDS             2068..>3189
                     /locus_tag="PLT35_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit family protein"
                     /protein_id="HOG29830.1"
                     /translation="MQNDFHRVAIVNRGEPAMRFIHAVREYNSEHRTDIRVIALFTEP
                     DRHARFVREADEAVDLGPSTFVDPRDGLRRPKYLDGGAVERALGDAQADAVWTGWGFL
                     AERPEFAERCERLGLTVIGPDGPSMRRLGDKICAKRLAEQVGVPVVPWGGGPADTVDA
                     ALDHAQRLGYPVGLKPSGGAGGRSIRRLAAPAELERAFAPVRRDAARNFLDRTLFIER
                     WMPGMRHIEVQIQADRHGTIWALGTRDCSLQRRFQKLAAEAPAVGLTPEVETALLDAA
                     VRIVAAARYRDQASVEFLVDLAGRFYFLEANTRLQVEHVVTELTSGIDLVKLEIDVAR
                     GGRLAPAPPAGSGHAVEVRLNAEDPYSGFAASPGVVTQFR"
BASE COUNT          479 a         1108 c         1155 g          447 t
ORIGIN      
        1 agagcggatc gaatcccatc acgcccagcg aaatcgtcga gcgcctgcgc cggctcgccg
       61 ccgcgccgaa ggccgaaacc gccaggtgac gagcgggccc cgtgcgccgg ggcccgagag
      121 aagagaggat cgcggacatg agtgtcctga ctgcgcagcc cgtgagtcag ttcgtggcga
      181 aggactacaa gagcgacctg aagcccatct ggtgcccggg ctgcggcgac ttcggcgtgg
      241 tgcaggcgat ctaccgggcc ctggccaaca tcggccgggc gccgcacgag atcgcattcg
      301 tctcgggcat cggctgctcg agccgcatcc ccgggtacac gacggcctac ggcttcaaca
      361 gcgtccacgg gcgcgcgctg cccatcgccc agggcatcaa gctcgccaac cccgacctgc
      421 tggtgctgtg cgcgggcgga gacggggacg ggttctcgat cggcggcggg cacgtggccc
      481 acgtcgtccg ccggaacatc gacctcacgt acatcgtgat ggacaaccag atctacggcc
      541 tcaccaaagg gcagttgtcg cctacgtcac gccgcggcct cgtcacggcc tcgtcggccc
      601 tcggctccat ggagggtccg gtcaaccccc tgatgtacgt gctcggctac ggcgcctcct
      661 tcgtcgccca ggggacgcca gcggacatgg cggggcttgc ctcgatcatc gaggaggcga
      721 tccggttccc gggcttcgcc ttcgtcaacg tccagtcgcc gtgcgtcacc tacgggcagg
      781 aagaccagtt gctgaaggcc cagaaggcga tgatgcagcc gctggcctcg ttgaaccacg
      841 accccgcgaa ccgtctggcg gcgctcgacc tggcccaggc ctacggcgag aagctctaca
      901 ccggggtctt ctaccgcaac cccgacccgc cgccgacgtt cgaggccctc gtccggcagc
      961 gccaggagca gcagcggccg ctcgcgagga gccgggcgga cgtgctgaac gtgttcaggc
     1021 tgaagacgcc gtagccaggc tcactcggcc atcacccggc ggtaccacac ggagtagtgc
     1081 cggatgcgcc ggggggcatg ggaaccgact gaggcgagcg caactgcggc aaggagcagc
     1141 gcgacgcggt gctcccacag gaacgggacg agcccgagca gcagcagctt ggcgtagacg
     1201 aacagcgcgc agacctgatg cgcccagtgc gccgtcgggt agatctcgac ggccatcatc
     1261 acggctccgg tcgcgacggc gacccagacc agcggcacca gccggcccgg gtcggccgca
     1321 acgacgtggc cgccgagcag cgtgcccgtc gcggcgagat gcaccgtccg cgcggcgatg
     1381 ttcaaggcgc gcccgtggcg cagacggcgg cgcggctgcg ggaagagcag ggcggtgagg
     1441 actcgcattc cgtgtgctcc gctcgtgagg ggcggcggcg cgcggccgcc ggtcgattat
     1501 agcgggggct ggcagcatga cggcgctgag catccggcag gcccgcagcg gcgacgccgt
     1561 ttgggcggcc gcgctgatgg cctcgtccga tccgtggatc acccttggcc gcggcttcga
     1621 ggcgtgccac caggcatgca cgtgggccgg cgacgtgctg gagatcgccg aactggaggg
     1681 cgagcgctgc ggcttcgtcc tcgtgcggcc ctccggcgtc gccggggcgc cttacgtcaa
     1741 gtccatcgcg gtcgcgccag ggttccggag ccgtggcgtc ggagcggcgc tgctcggcca
     1801 cgtcgagcgg acgtatgcgg ggcgtgcccg ccacctgttc ctctgcgtct cctcgttcaa
     1861 cgaccgggcc aggctgttct acgaacgcca gggctacgcc gcggttggcg cgatcaagga
     1921 cttcctcatc gagggcgccg acgagatcct gatgcagaaa cggctgccgg cgccggacgc
     1981 ggcagcatga tgtcctaaga tgagggggtg cccgtgtccg gcggccggcg tcgccggcgc
     2041 gccggccgag cgggactgag ggcttccatg cagaacgact tccatcgcgt ggccatcgtc
     2101 aaccgcggcg agcccgccat gcgtttcatc cacgcggtgc gcgagtacaa cagcgaacac
     2161 cggacggaca tccgcgtgat cgcgctgttc accgagcccg atcgccacgc ccggttcgtc
     2221 cgggaagcgg acgaggccgt ggatctcggc ccctccacgt tcgtggatcc gcgtgacggg
     2281 ctgagacgcc cgaagtacct ggacggcggg gccgtcgagc gggcgctcgg cgacgcgcag
     2341 gcagacgccg tctggacggg gtggggattc ctcgccgagc ggccggaatt cgccgagcgc
     2401 tgcgagcgcc tcgggctgac cgtcatcggc ccagacgggc cgtcgatgcg gaggctggga
     2461 gacaagatct gcgccaagcg cctggccgag caggtgggcg tgccggtggt tccctggggc
     2521 ggcgggcccg ccgacaccgt tgacgccgct ctggaccacg cgcagcggct gggctatccc
     2581 gtcggcctca agccgagcgg cggcgctggc ggccggtcga tcaggcgcct ggcggcgccg
     2641 gccgaactcg agcgggcgtt cgctccggtc aggcgcgacg cggcccgcaa cttcctggat
     2701 cgcacgctgt tcatcgagcg ctggatgccc ggcatgcgcc acatcgaggt gcagattcag
     2761 gcggaccgcc atggcacgat ctgggctctt ggcacgcgcg actgctccct gcagcggcgg
     2821 ttccagaagc tggcggcgga agcgcccgcc gtcggcctga ccccggaggt ggaaacggcg
     2881 ctcctcgacg ccgccgtgcg catcgtcgcc gcagcccgct accgcgatca ggcgtcggtg
     2941 gagttcctgg tcgatctggc cgggcggttc tacttcctcg aagcgaacac gcgcctgcag
     3001 gtggagcacg tggtcacgga gctcacgagc ggcatcgacc tggtgaagct cgagatcgac
     3061 gtcgcgcgcg gcgggcgcct cgctccggct ccgccggccg gctcgggcca cgcggtcgag
     3121 gtgcggctga acgcggaaga tccctacagc ggcttcgccg cttcgcccgg cgttgtgacc
     3181 cagtttcgg
//
LOCUS       DAOSIF010000370         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26445_length_3187_cov_3.720307, whole genome shotgun sequence.
ACCESSION   DAOSIF010000370 DAOSIF010000000
VERSION     DAOSIF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26445_length_3187_cov_3.720307"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PLT35_11185"
     CDS             complement(<1..852)
                     /locus_tag="PLT35_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG29831.1"
                     /translation="MRTRVAVGIDIGGTNSVLGLVDEQGACLAESLVDTVPEAGPESL
                     VERLVSAVRELHAPLAGTCEIAGVGIGAPNANYYTGTVEHPPNLGWKGVTPFVALVRR
                     HVDVPVVITNDANAAALGELRFGAARGMRDVIVITLGTGLGSGIIANGELIYGVDGFA
                     GEIGHVTVQPDGRECGCGRRGCLETYASATGICRTVFELLSTSRAESELRDVPFRKLT
                     GRLISAAAHRGDPIALAAFEHTGKVLGTTLANTVAHTAPEAIILFGGLAGAGELIFEP
                     TRRWLEAH"
     gene            complement(855..2174)
                     /locus_tag="PLT35_11190"
     CDS             complement(855..2174)
                     /locus_tag="PLT35_11190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOG29832.1"
                     /translation="MAHGGPAAAGARGAAPESPNYTPALVVLTSLFFMWGFITCLNDI
                     LIPHLKQLFTLNYTQVMLIQFTFFAAYFVVSLPSGFVVEAVGYQRGIVIGLITAGVGC
                     LLFYPAAGTRSYPIFLGALFVLASGITLLQVSANPYVAILGPPRTASSRLTLTQAFNS
                     FGTAIAPVFGSFLILSVAGKTAEEMAALGPSDLAAYQAAEKASVQDPYLGLAAALFAI
                     AAVFARVKLPAIEASSGDARSDTGGSYDAQHPSAWKYRHLALGAIGIFLYVGAEVSIG
                     SFLVNFLGEPSIAALPAAEAGRYLSFYWGGAMVGRFVGSAAIRRVRPNRALALSAASA
                     ALLVLVTMLTSGRVAMWAILAVGLFNSIMFPTIFTLALDGLGRHTGQGSGILCMAIVG
                     GAVVPVIQGAFADAIGIHRAFFLPILCYAFILYYGLKGYEPSYVKAV"
     gene            complement(2257..2703)
                     /locus_tag="PLT35_11195"
     CDS             complement(2257..2703)
                     /locus_tag="PLT35_11195"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG29833.1"
                     /translation="MSDESISTVSLKLKENYEFLAAFEALPDVAPLLLDEPPPLGGAS
                     GPSASALLGAAVGNCLAASLLFCLRRSRVEVAGAEAKVAVRVARSEKGRLRITGISVS
                     LEPELAPGQDPAKYDRCLGIFEDFCVVTQSVRAGVPVEVNVSPRQV"
     gene            complement(2822..>3187)
                     /locus_tag="PLT35_11200"
     CDS             complement(2822..>3187)
                     /locus_tag="PLT35_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29834.1"
                     /translation="GGTYSNLFGAHPPFQIDGNFAATAGIAEMLLQSQGGAIVLLPAL
                     PGAWRDGSVRGLRARGGFEVDLAWRGGALDRITLRSTAGGRAQLRHGVRSRIVSVPAG
                     GLVTLDGQLEPALLSPALR"
BASE COUNT          512 a         1141 c         1117 g          417 t
ORIGIN      
        1 gtgcgcctcc agccagcgcc gcgtcggctc gaagatcagt tcgccggcgc cggcgaggcc
       61 gccgaacagg atgatggcct cgggcgccgt gtgggcgacg gtgttggcca gcgtcgtacc
      121 caggaccttc cccgtgtgct cgaaggcagc cagagcgatc ggatcgcccc ggtgcgccgc
      181 cgcggagatc aggcgcccgg tcagtttgcg gaacggcacg tcccggagct cgctctccgc
      241 cctcgacgtg ctgaggagct cgaagaccgt gcggcagatg cccgtcgccg aggcgtaggt
      301 ctcgaggcac cctcgccgcc cgcatccgca ttcgcggcca tccggttgca ccgtgacgtg
      361 gccgatctcg ccggcaaacc cgtccacgcc gtagatcagc tccccgttgg cgatgatccc
      421 gctgccgagg ccggtgccga gcgtgatcac aatcacgtcg cgcatgcccc tggccgcgcc
      481 gaaccgcagt tcgccgaggg ccgccgcgtt cgcgtcgttc gtgatgacga cggggacgtc
      541 gacgtgccgc ctgaccaggg ccacgaaggg cgtgacgccc ttccagccga ggttgggcgg
      601 gtgctcgacg gtgccggtgt agtagttggc gttcggcgcc ccgatgccga ccccggcaat
      661 ctcgcaggtg ccggccagcg gggcgtgcag ctcgcgaact gccgaaacca gccgctcgac
      721 gagcgactcc gggccggctt cggggaccgt gtcgaccaac gactcggcga ggcacgctcc
      781 ctgctcgtcc accagcccca gcacgctgtt tgtgccgcct atgtcgatgc cgaccgccac
      841 tctggtgcgc atgatcagac cgccttcacg tacgagggct cgtagccctt gaggccgtag
      901 tagaggatga acgcgtagca gaggatcggc aggaagaagg cccggtggat gccgatcgcg
      961 tccgcgaacg cgccctggat gaccggcacg accgcaccgc cgacgatggc catgcagagg
     1021 atgcccgagc cctggcccgt gtgcctgccc aggccgtcca gcgccagcgt gaagatcgtg
     1081 gggaacatga tcgagttgaa cagcccgact gccaggatgg cccacatcgc cacccggccg
     1141 gacgtcagca tcgtgacgag cacgagaaga gcggcagagg cagcgctgag ggccagcgcc
     1201 cggttgggcc tcacgcggcg gatggccgcg gacccgacga accggcccac catggcgcct
     1261 ccccaataga aagacagata tcgccccgcc tccgccgcgg gcagcgccgc gatggagggc
     1321 tcgccgagga agttcaccag gaaactgccg atggacacct cggcgccgac gtagaggaaa
     1381 atgccgatcg ccccgagcgc gaggtgccgg tacttccacg cgctcgggtg ctgcgcgtcg
     1441 tagctcccgc cggtgtcaga ccgggcgtcg cccgacgagg cctcgatcgc gggcagcttc
     1501 acccgggcga agaccgccgc gatcgcgaag agcgcggcgg cgaggcccag gtacgggtcc
     1561 tggaccgacg ccttctcagc cgcctggtac gccgcgagat ccgacgggcc gagggcggcc
     1621 atctcctcgg cggtcttgcc cgcaaccgac aggatcagga acgagccgaa caccggcgcg
     1681 atggccgtgc cgaaggagtt gaaggcctgc gtcagcgtca gccggctcga ggccgtcctc
     1741 ggcggcccca ggatcgccac gtacgggttc gccgacacct gcagcagcgt gatgccggac
     1801 gccagcacga agagcgcccc caggaagatc gggtacgagc gcgtcccggc ggccggatag
     1861 aagagcaggc agccgacgcc agccgtgatg aggccgatga cgatcccgcg ctggtagccc
     1921 actgcctcca cgacgaagcc cgaggggagc gacaccacga agtacgccgc gaaaaaggtg
     1981 aactggatga gcatcacctg ggtgtagttc agcgtgaaca gctgcttcag gtgcgggatc
     2041 aggatgtcgt tcaggcacgt gatgaagccc cacatgaaga agagggacgt cagcaccacg
     2101 agcgccggcg tgtagttcgg gctctccggc gccgcgcctc gcgcgccggc cgcggcgggt
     2161 cctccatggg ccatgtctca tctccagcgg gctgcgcggg cgccccagga ggcgccggcg
     2221 gcccccggcg ccttcgcccg gcccgtgcgc cctccctcag acctgccgcg ggctgacgtt
     2281 cacctcgacg ggaacgccgg cccgcacgct ctgcgtgacg acgcagaagt cctcgaagat
     2341 cccgaggcag cggtcgtact tggccgggtc ctggcccggc gcgagttcgg gttcgagcga
     2401 cacgctgatg cccgtgatgc gcaggcgtcc cttctcggag cgagccacgc gcaccgcgac
     2461 cttggcctcg gcgccggcca cctcgacgcg cgagcggcgc aggcagaaca ggaggctggc
     2521 cgcaaggcag ttgcccacgg ccgctccgag gagggcggac gcgctcggcc cgctggcccc
     2581 gccgagcggc ggcggctcgt ccagcaggag gggcgcaacg tccgggagcg cctcgaacgc
     2641 ggcgaggaac tcgtagttct ccttcagctt gagcgatacc gtgctgatcg actcgtcgga
     2701 catgccattc ctcccgctga ctggagccgc caggcgccgc ctgcgtcgac aacggccgtg
     2761 gcagcccggg cggccgccgg tgccatgccg cagccgactc tactggagtc acgcaatcgg
     2821 atcaacgaag cgccgggctg agcagcgccg gctcgagctg cccgtccagc gtgacgaggc
     2881 ctccggcggg cacggagacg atcctgcttc gcaccccgtg ccggagctgc gcgcggcctc
     2941 ccgccgtcga gcgcagcgtg atccggtcga gcgcgccgcc ccgccaggcg agatccacct
     3001 cgaagccgcc tcgggcgcgc agcccccgca ccgagccgtc gcgccaggcg ccgggcagcg
     3061 ccggcagcag gacgattgct ccgccctggc tctggagcag catttccgcg atgccggccg
     3121 tcgcggcgaa gttcccgtcg atttggaacg gcgggtgcgc gccgaagagg ttcgagtacg
     3181 tgcctcc
//
LOCUS       DAOSIF010000371         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26482_length_3184_cov_3.121445, whole genome shotgun sequence.
ACCESSION   DAOSIF010000371 DAOSIF010000000
VERSION     DAOSIF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26482_length_3184_cov_3.121445"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2294)
                     /locus_tag="PLT35_11205"
     CDS             complement(<1..2294)
                     /locus_tag="PLT35_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG29835.1"
                     /translation="MRLARLLLLPALLASAVPAEAQHLLITGRTVDAETGAAVAARVE
                     APDLGVSVTAGADGRFVLDAGPVGRATLVVSCPGYYVQRTLVQSAAPLDIRLVPVITL
                     SDRVEVTAGRAREGADPVTFSNVPRQAIADAHWGQDPAILLSALVPGVFARNDSGNGI
                     GYSYFSIRGFGQARTRVTLNGAPINDAESGELFFVDLADFMTTAGDVQVRRGVAGLTG
                     LGGAIDVTTAQPSVEPGFSLDAGAGSFGTSRLSLVWESGLLNGAWALSARYSKIRTDG
                     YRDQAWADMWNYYVSIARFGGRSRLRAVLFGGPEDAHLAYDGIDRATLEGGVTGDADR
                     DRRTNPLSYPGEIDHFFQPHYQIVHDVRVSERTSFDQTLYAFRGTGYYDQFRARRWLV
                     EYGLPDAALPDGTSVRRSDLVRRRSVDEWDVGWVPKLSHRAGRVALDLTGEWRWHRAH
                     HTGEVRWAQFLPAGVGAGHRYYDYRVGKQTATAAMTAKADVTPRITASAGFQLSRHAY
                     ELFDDRLKGVAFTRAYGFVLPRAGVVVKLADGADAYATAARGMREPFFRNIYDPQDYY
                     ATGPADLDPEDVWNVETGTSWRRGRWRARANAYWMHFRNEIVYAGALDDNGVPVYGNG
                     ARSRRLGLEVDAGATVSSRIGVEAAVSLARNTFLRYREHDWEGGTAVYDGKRAAVFPG
                     VMASAAAHANLGPARVSLSLRHVGRFYLDNTENAGRINDAWTTADAAARVALPARALR
                     TLGLDRWALDLRVNNLLDRRYTSFG"
     regulatory      complement(2281..2379)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     assembly_gap    2637..2736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2839..>3184)
                     /locus_tag="PLT35_11210"
     CDS             complement(2839..>3184)
                     /locus_tag="PLT35_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG29836.1"
                     /translation="MCGATLVLLHVADGWAARAYDELNLRESKEIREDREYLEGLRAS
                     LASEGFAVEARLAKGDPADEIVRAADAERADLVAMATHGHRLIKDIILGATVDKVRHL
                     LKVPVLLLQSRK"
BASE COUNT          414 a         1101 c         1139 g          430 t
ORIGIN      
        1 ccgaaggacg tgtagcgccg atcgagcagg ttgttgaccc tcaggtcgag ggcccagcga
       61 tcgaggccga gcgtgcgcag ggcccgggcc ggcagtgcga ccctggccgc ggcgtccgcc
      121 gtggtccagg cgtcgttgat ccgtccggcg ttctccgtgt tgtcgaggta gaaccggccg
      181 acgtgccgga gtgagagcga gacgcgggcc gggccgaggt tcgcgtgcgc cgcggccgac
      241 gccatgacgc ccggaaagac ggccgcccgc ttgccgtcgt agacggccgt gcccccctcc
      301 cagtcgtgct cgcggtagcg gaggaatgtg ttgcgcgcca gcgagacggc ggcttccacg
      361 ccaatccggc tcgacaccgt cgcgcccgcg tccacttcca ggcccagccg ccgcgagcgg
      421 gcgccattgc cgtagacggg gacgccgttg tcgtcgagcg ccccggcgta gacgatctcg
      481 ttgcggaagt gcatccagta ggcgttcgcg cgcgcccgcc accggccgcg gcgccaggac
      541 gtgccggttt ccacgttcca gacatcctcc ggatcgaggt ccgccgggcc cgtcgcgtag
      601 tagtcctggg ggtcgtagat gttgcggaag aagggctccc gcatgccgcg ggccgccgtg
      661 gcgtaggcgt cggcgccgtc ggccagcttc acgaccacgc cggcgcgcgg gaggacgaag
      721 ccgtaggccc gcgtgaacgc gacgcccttc aggcggtcgt cgaagagctc gtaggcgtgg
      781 cgcgagagct ggaagccggc gctcgccgtg atgcgcggcg tgacgtcggc cttcgcggtc
      841 atggcggccg tcgccgtctg cttgccgacc cggtagtcgt agtagcggtg cccggccccg
      901 acgccggccg gaaggaactg cgcccacctg acttcgccgg tatggtgcgc ccggtgccag
      961 cgccactccc ccgtcaggtc cagcgcgacg cgcccggcac ggtgcgaaag cttcggcacc
     1021 cagccgacat cccactcgtc gacgctccgg cgcctgacca gatcgctccg ccggacgctc
     1081 gtgccgtcgg ggagcgccgc gtccggcagg ccgtattcga cgagccagcg gcgggcgcgg
     1141 aactggtcgt agtagcccgt tccgcggaac gcgtagagcg tctggtcgaa cgacgtgcgc
     1201 tccgagaccc tgacgtcgtg gacgatctga tagtgcggct ggaagaagtg atcgatctcg
     1261 cccggatagg acagcgggtt cgtgcgccgg tcgcgatccg cgtcgcccgt cacgccgcct
     1321 tcgagcgtcg cgcggtcgat gccgtcgtag gccagatgcg cgtcttcggg cccgccgaag
     1381 agcaccgccc gcagccgcga ccggccgccg aaccgcgcga tcgacacgta gtagttccac
     1441 atgtccgccc acgcctggtc gcggtagccg tcggtccgga tcttcgagta ccgggccgag
     1501 agcgcccagg cgccgttcag gaggccggac tcccagacga gcgacaggcg gctggtgccg
     1561 aacgacccgg cgccggcgtc gagcgagaag ccgggttcga ccgacggctg ggccgtcgtg
     1621 acgtcgatgg cgccgccgag gcccgtgagg ccggccaccc cgcgccgcac ctgcacgtct
     1681 cccgccgtcg tcatgaagtc ggcgagatcg acgaagaaca gctcgccgga ttcggcgtcg
     1741 ttgatcggcg cgccgttcag cgtcacgcgg gtccgcgcct ggccgaagcc gcggatcgag
     1801 aagtacgagt agccgatgcc gttgccgctg tcgttcctgg cgaacacgcc gggcacgagc
     1861 gcggagagca ggatcgccgg gtcctggccc cagtgcgcgt cggcgatggc ctggcgcggc
     1921 acgttcgaga aggtgaccgg gtcggcgcct tcgcgcgcgc ggccggcggt gacctcgacg
     1981 cggtcgctca gcgtgatgac gggcaccagg cggatgtcga ggggtgcggc cgattggacg
     2041 agcgtgcgct ggacgtagta gccggggcac gacacgacca gcgtcgcgcg cccgaccggc
     2101 ccggcgtcga ggacgaaacg cccgtcggcg cccgcggtca cggacacgcc caggtccggc
     2161 gcctcgacgc gcgcggcgac ggccgcgccg gtttcggcgt cgacggtgcg gccggtgatg
     2221 agcaggtgct gggcctcggc cggcacggcc gaggccagca gcgcgggcag caggagaagg
     2281 cgtgcgagtc tcatcgttcc ctacgccggc atgacccgga tcaggttcag agggtgtgat
     2341 ctcagccccg cggcgtgcgg gacaccccta cgacggtgac gatactgtac tgtgacgcca
     2401 acgggcgctc agggtcaagc gcgcggcgcg ggatctccgc cacccgctgt cacccgcgct
     2461 cgctcgtctt gttcgcgccg gagggagccg ggtcctgtcg ccagtcaccc ccgtccgtgc
     2521 tcgggcgcaa agcgcgtgtc acgggagtcg ccctgcggcc ggccgggggg cccccgactg
     2581 gcgccacccg ctgtcacccg cgctcgctcg tcttgttcgc gccggaggga gccgggnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcg ccacccgctg tcacccgcgc
     2761 tcgctcgtct tgttcgcgcc ggagggagcc gagccctgtc gccgaccatg cccgccggga
     2821 gcgcgggccg tcgcccgcct acttgcgcga ctgcagcagc aggacgggca ccttgagcag
     2881 gtggcgcacc ttgtccacgg tcgcgccgag gatgatgtcc ttgatgagac ggtggccgtg
     2941 cgtagccatg gcgaccaggt cggcccgctc ggcgtcagcc gcgcggacga tctcgtcggc
     3001 cgggtcgccc ttggcgaggc gcgcctccac ggcgaagccc tccgaggcga gactcgcgcg
     3061 caggccctcg aggtactcgc ggtcctcgcg gatctccttc gactcgcgca ggttgagctc
     3121 gtcgtaggcg cgggcggccc atccgtccgc gacgtgcagc agcaccagcg tggcgccgca
     3181 catc
//
LOCUS       DAOSIF010000372         3152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26825_length_3152_cov_4.208589, whole genome shotgun sequence.
ACCESSION   DAOSIF010000372 DAOSIF010000000
VERSION     DAOSIF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26825_length_3152_cov_4.208589"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLT35_11215"
     CDS             <1..528
                     /locus_tag="PLT35_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOG29837.1"
                     /translation="RLAKIVGGVAVIKVGAATETEMKEKKARVEDAMHATKAAVEEGI
                     VAGGGVALIRAIKTLDEVKAAGDQLVGVNIVRRAVEEPLRWIAQNAGFEGSIVVQKVK
                     EGNDDFGFNALTDEYEHLVAAGVIDPAKVVRSALQNAASIASLLLTTEAMVADIPEEK
                     KEPAVPAGGGMGGMY"
     gene            complement(703..1626)
                     /locus_tag="PLT35_11220"
     CDS             complement(703..1626)
                     /locus_tag="PLT35_11220"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG29838.1"
                     /translation="MPERVAADRAPREIPYWVSMRLDPGRPENQLFADQPGRRVSKAL
                     GVSGFLHAAAVAGVFLLVAWLPEPAPLDLRRASSDEIPRERIVWLNEPGPGGGGGGGG
                     NRSIEPPRLAEAPGKDRLTVPVAKPPEIVPVPSPEDEMPKPELNIPAKAMESSDQLLA
                     GVLDGMKSSNSPTSQGPGSGGGSGTGVGTGIGSGTGSGLGPGSGGGTGGRYYRPGSGI
                     ELPRLLREVKPQYTTDAMRAKIQGTAVLDCVVGTDGSVRECDVVRSLDSSFGLDQEAL
                     KAARQWRFVPGKRFGQPVPVLVTIELTFTLR"
     gene            complement(1818..2276)
                     /locus_tag="PLT35_11225"
     CDS             complement(1818..2276)
                     /locus_tag="PLT35_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29839.1"
                     /translation="MPQSEPVLFTVGVFQDLAWAERGIEALKKQGFDAGALTVAGKLT
                     PELVAAIERAVGGVPGRWELPGIGPLVAHGSLSAVLDGPAKDLGRTGLAAAMRRAGFQ
                     PHDGLIYETLVERGGVLVAVEDAPRAADALSVLLSYGAGNSAIGAWQKRL"
     gene            2439..2591
                     /gene="tatA"
                     /locus_tag="PLT35_11230"
     CDS             2439..2591
                     /gene="tatA"
                     /locus_tag="PLT35_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOG29840.1"
                     /translation="MNLGLPELLVILAIIILVFGANRLPDLGRGIGKAIRNFRDATAE
                     GREKRD"
     gene            complement(2616..>3152)
                     /locus_tag="PLT35_11235"
     CDS             complement(2616..>3152)
                     /locus_tag="PLT35_11235"
                     /inference="COORDINATES: protein motif:HMM:NF020671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HOG29841.1"
                     /translation="HPLEEGMPREEARERVCGRAAAGVFERALEGLVQTGRIAVTDRI
                     ALTTHRVEAGSAEAAARDAIEARFREAGLRPPDQAALAAELGRPPSAVAAVVALLVRQ
                     RVLTRVDTLVFHETALARLKEDLRGMKAGSAGAAVPIDVAAFKARYGITRKYAIPLLE
                     YLDRERVTRRTGEGRVLT"
BASE COUNT          506 a         1153 c         1047 g          446 t
ORIGIN      
        1 cggctcgcca agatcgtggg cggcgtggcc gtgatcaagg tcggggccgc caccgagacc
       61 gagatgaagg agaagaaggc ccgcgtcgag gacgcgatgc acgccacgaa ggccgcggtc
      121 gaggagggca tcgtcgccgg cggcggcgtg gcgctcatcc gcgccatcaa gacgctcgac
      181 gaggtcaagg ccgccggcga ccagctcgtc ggcgtcaaca tcgttcgccg cgccgtcgag
      241 gagccgctgc gctggatcgc gcagaacgcc ggcttcgaag gctcgatcgt cgtgcagaag
      301 gtcaaggaag gcaacgacga cttcggcttc aacgcgctca ctgacgagta cgagcacctc
      361 gtcgccgccg gcgtcatcga cccggccaag gtggtccgct cggccctgca gaacgccgcg
      421 tcgatcgcgt cgctgctcct gacgaccgaa gcgatggtcg ccgacatccc cgaggagaag
      481 aaggagcccg cggtgccggc cggcggcggc atgggcggaa tgtactagag gctaggaact
      541 ggggactggg ggccaaggac tcgtcgccac tcagcagcca gtagccaata gcatcggaac
      601 gacgcacgca gaacggcccg gcgagcaagg tgctcgccgg gccgtttctt cgtgcgcttt
      661 gaacggtctg ttccccagcc cccggccccg aatccctcgt ccctagcgca gcgtaaaggt
      721 gagttcgatt gtgacgagga ccggcacggg ctggccgaag cgcttgccgg gcacgaaccg
      781 ccactggcgc gcggccttga gggcctcctg gtccaggccg aaactcgagt cgagcgagcg
      841 aaccacatcg cactccctga cagacccgtc ggtgccgaca acgcaatcga gaactgcggt
      901 gccctggatc ttcgcccgca tcgcatcggt cgtgtactgg ggcttgacct cacgcagaag
      961 gcgcggcagc tcgatgccgg atcccggccg gtagtaccgt ccgccggtgc ctccgccact
     1021 gcccgggcca agacccgaac ccgtgccaga cccgatcccg gttccgaccc cggtgccgga
     1081 gcctccgccg ctccctggcc cttgcgaagt cggggagttc gatgacttca tgccgtcgag
     1141 gaccccggcc agcagctggt ccgacgattc catcgccttc gcggggatgt tcagctccgg
     1201 cttgggcatc tcgtcctcag gcgaaggcac cggaacgatc tccgggggct tcgccaccgg
     1261 caccgtcaac cgatccttgc cgggcgcctc ggccaggcgc ggcggctcga tcgatcggtt
     1321 gccgcccccc ccgcctccgc ctcccggccc gggctcgttg agccagacga tccgctctct
     1381 cgggatctcg tcgctgctgg cgcgcctgag atcgagcggc gccggctcgg gcagccaggc
     1441 cacgagcagg aacacgcccg ccacggccgc cgcgtgcagg aacccggaca ccccaagggc
     1501 cttcgatacc cggcgtcccg gctgatcagc aaacagctgg ttctcgggcc ggcccggatc
     1561 cagacgcatc gacacccaat acgggatctc ccggggggcg cggtctgcgg caactcgttc
     1621 aggcatcggc acggaactcg cttcgtccaa ttgtcgcgcg tcgaaccgcc aaccgcaagt
     1681 gcaagagcgg tgccggaacg cgcctgccgg atccccaagc gcgccgcttg ttcgacgagg
     1741 gccgcgcgag gatgtcctgt cgaagctgcc agcggtgtca tccgctggcg acatggacga
     1801 gcggtctgag agcagcgcta gaggcgcttc tgccacgcgc cgatggcgga attcccggcg
     1861 ccgtaggaca gaagcactga cagggcgtca gcggcgcgag gcgcgtcctc aaccgcgacc
     1921 agcacgccgc ccctctccac gagcgtttcg tagatcaggc cgtcgtgggg ctggaagccg
     1981 gcccgccgca tagcggcggc caggcccgtc cggccgaggt ctttcgccgg accgtcgagc
     2041 accgcggaga gcgagccgtg cgcgaccagc ggaccgatgc cgggcagttc ccacctgccg
     2101 ggcaccccgc cgaccgcgcg ctcgatcgct gcaaccagtt ccggcgtcag cttgccggcc
     2161 acggtcagcg cgccggcgtc gaagccctgc ttcttcagcg cctcgatccc gcgttccgcc
     2221 catgccagat cctggaacac gcccacggtg aacagcaccg gttcgctctg aggcatcctg
     2281 cactccccgc agatcgcccc gtcacatcac ggggcgctcg acccgggcag gcgcctgcac
     2341 ggcgaaccgg cccgcagccg ttttcctact ctaagctcga acagcagccg cttcaagtgc
     2401 gcctgacggc tccctgggga agtgctacaa ttcccagtat gaatcttggg ctgcccgaac
     2461 tgctcgtcat cctggccatc atcatcctgg tcttcggggc gaaccgcctc ccggacctgg
     2521 gccggggcat cggcaaggcg atccggaact tcagggacgc cacggccgaa ggccgcgaga
     2581 aacgcgactg accgggcgac ggcccgctgc ccacctcaag tcaggacgcg gccttcaccc
     2641 gtccgccgcg tcacgcgctc tcgatcgagg tactcgagca agggaatcgc gtacttccgc
     2701 gtgatgccgt agcgcgcctt gaaggccgca acgtcgattg ggacggcagc gcctgccgag
     2761 cccgccttca tccctcgcag gtcctctttc agcctggcaa gcgcggtctc gtggaacacg
     2821 agggtgtcga ccctcgtcag cacgcgctgc cggacgagca gggccacgac ggcggccacc
     2881 gccgagggag gccggcccag ttcagccgcc agcgcagcct ggtctggcgg ccggagcccc
     2941 gcctcgcgga agcgcgcctc gatggcatcg cgggccgcgg cctcggcgct tcccgcctcg
     3001 acgcggtgcg ttgtgagcgc gatgcgatcg gtgaccgcga tgcgcccggt ctgcacgagc
     3061 ccctcgaggg cccgctcgaa gacgccggcg gcggcgcgtc cgcagacgcg ctcgcgcgcc
     3121 tcttcgcgcg gcatcccctc ctcgagcgga tg
//
LOCUS       DAOSIF010000373         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_26839_length_3151_cov_2.816214, whole genome shotgun sequence.
ACCESSION   DAOSIF010000373 DAOSIF010000000
VERSION     DAOSIF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26839_length_3151_cov_2.816214"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLT35_11240"
     CDS             complement(<1..865)
                     /locus_tag="PLT35_11240"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOG29842.1"
                     /translation="MAFSGTGRIWMNGSFVEWADAKVHIATHALHYGSAVFEGARCYD
                     TPKGSACFRLDAHMRRLTESARIYRMELAYSQAQLETAVLDTIRANGMKACYIRPLAY
                     RGYGALGVNPFPCPVDVAILVWEWGTYLGEEALERGVDVQVSSWSRMAPDSFPSLAKT
                     SANYANAALIKMDAIVNGYAEGVALDSFGYISEGSGENIFVVRDNVIYTPPVAASILP
                     GITRDSVITLARGLGYCVREEMLPREMLYVADEVFFVGTAVEITPVRSVDRIVVGAGH
                     RGPVTKALQEAY"
     gene            complement(955..1938)
                     /locus_tag="PLT35_11245"
     CDS             complement(955..1938)
                     /locus_tag="PLT35_11245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOG29843.1"
                     /translation="MFFRGQTIGKYKILSALGSGGFGTVYLAEDTWIGKKVALKVPHR
                     QSVNFGELLREPRLLAVLNHPNIVAVLTAEKQDSVFFIVMEYVPGETLESIIGRDGAL
                     ELTRALDLTCQVCNALDHAHRQGVLHRDLRPANIIVSDTGLAKVADFGTSRFLELAAI
                     GTTVIGSPPYMAPEQFHGRATFASDIYSLGVTMYQMMTGVLPYQTPAPGDLERLMRGE
                     LVAPPRLRNARIPPRVNDIILKAMAPDVSERYPRAADVLEDLLGADRPASRRPTDRPT
                     PSPAAAPVAAGEAPQAGWARTRDVPQPKFCRQCHKPLHARTDRCPFCGEAQ"
     gene            1985..>2666
                     /locus_tag="PLT35_11250"
     CDS             1985..>2666
                     /locus_tag="PLT35_11250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG29844.1"
                     /translation="MVPRLYVPSLAPGARTATLPPDEADHVRRVLRARVGSPVRVFDG
                     RGAEREARVDSLEGRTVTVVLGDAVAPARECRVSVTLGVAVLKGEKTDAVVRDAVMMG
                     VAALQPMLTDRSDLPPAAVARGTRLDRWRRIAVASAKQCGRAVVPEVFAPSSLPECLA
                     RHRRDAGVVLVEPSSEGLARARRPVPRAPASALVLVGPEGGWTRGEVDAAVASGCAAV
                     TLGPRTLRA"
     assembly_gap    2667..2676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2709..>3151)
                     /locus_tag="PLT35_11255"
     CDS             complement(2709..>3151)
                     /locus_tag="PLT35_11255"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG29845.1"
                     /translation="RRRVLDVGTGSGVLAFAACALGAREALGVDSDADAIENARGNAE
                     LNAGLPAVRFEEADFRSRAIAPADIVLANLTGALLMSAAGALTALVAPGGTLVVSGFT
                     PDERPQVLAAFAPWGEAVWQRQEDGWCAAMVRGGSARYTISSPQ"
BASE COUNT          416 a         1124 c         1137 g          464 t
ORIGIN      
        1 aatacgcctc ctgcagcgcc ttcgtgacgg ggccgcggtg ccccgcgccg acgacgatac
       61 ggtcgacgga acggaccggc gtgatctcga cggccgtgcc cacgaagaac acctcgtcgg
      121 cgacgtagag catctcgcgg ggcagcatct cctcgcgcac gcagtacccg aggccgcgtg
      181 ccagcgtgat caccgaatcg cgcgtgatcc ccggcaggat cgacgccgcc acgggcggcg
      241 tgtagatgac gttgtcgcgc accacgaaga tgttctcgcc cgagccctcg gagatgtaac
      301 cgaagctgtc cagcgcgacc ccttcggcgt agccgttgac gatcgcgtcc atcttgatca
      361 gcgcggcgtt ggcgtagttc gcgctggtct tcgcgagcga gggaaaggag tccggcgcca
      421 tccgcgacca ggagctcacc tggacgtcca ccccccgctc gagggcctct tcaccgaggt
      481 acgtgcccca ctcccagacc aggatcgcga cgtccaccgg acaggggaac ggattgacgc
      541 cgagcgcccc gtagccgcga taggccagcg ggcggatgta gcaggccttc atgccgtttg
      601 cgcggatggt gtcgagtacc gccgtctcga gttgcgcctg ggagtaggcc agctccatcc
      661 ggtagatcct ggcggactcc gtcagccggc gcatgtgcgc gtccagccgg aagcaggccg
      721 accctttcgg cgtgtcgtag cagcgggcgc cctcgaacac cgcgctgccg tagtgcaggg
      781 cgtgggtggc gatgtggacc ttcgcgtcgg cccactcgac gaagctcccg ttcatccaga
      841 tcctgccggt tcctgagaag gccatcgcgc gctccttggc gtgaagggtg gtgctgcaag
      901 agccgtgctc ggcgcccgcg cccgccggcc cgcacaggcc acccggctgc cattctactg
      961 tgcctcgccg cagaacgggc agcggtcggt ccgggcgtgg aggggcttgt ggcactgtcg
     1021 gcagaacttc ggctggggaa catcgcgggt cctcgcccag ccggcctgcg gggcctcgcc
     1081 cgccgcgacc ggcgccgccg ccggagacgg cgtcggccgg tccgtggggc gcctggacgc
     1141 gggccggtcc gctccgagca ggtcttcgag aacgtccgcc gcgcgcgggt agcgctcgga
     1201 gacgtccggg gccatcgcct tgaggatgat gtcgttcacc cggggcggga tgcgcgcgtt
     1261 ccgcagccga ggcggcgcca ccagctcgcc gcgcatcagg cgctcgaggt cgcccggcgc
     1321 cggcgtctgg tagggcagga cgcccgtcat catctggtac atcgtgacgc cgagcgagta
     1381 gatgtccgag gcgaaggtcg cgcgcccgtg gaactgctcg ggagccatgt acggcgggct
     1441 gccgatcacg gtggtgccga tggccgccag ctccaggaag cgcgacgtgc cgaagtccgc
     1501 taccttcgcc agcccggtgt ccgacacgat gatgttcgcc ggccggagat cgcggtgcag
     1561 cacgccctgg cggtgcgcgt ggtcgagggc gttgcagacc tggcacgtga ggtcgagcgc
     1621 ccgcgtcagt tccagggccc catcgcgccc gatgatcgac tcgagggtct cgccaggcac
     1681 gtactccatc acgatgaaga acacgctgtc ctgcttctcc gccgtcagga cggccacgat
     1741 gttcgggtga ttgagcacgg cgagcaggcg cggctcgcgg agcagttcgc cgaagttcac
     1801 gctctggcgg tgggggacct tcagcgcgac tttcttcccg atccacgtgt cttcggccag
     1861 gtacaccgtg ccgaatccgc cgctgccgag ggccgacagg atcttgtact tgccgatggt
     1921 ttgtccgcga aagaacatgc agcgcctgcc ggggagtata gcgcggcttg tttataattg
     1981 ccgcatggtg ccgcgcctct acgtgccgtc gctcgcgccc ggcgcccgca cggccacgct
     2041 gcccccggac gaggccgacc acgtcaggcg cgtgctgcgg gccagggtcg gctcgcccgt
     2101 gcgggtcttc gacggccgcg gcgccgagcg cgaggcccgc gtggactcgc tcgagggccg
     2161 gaccgtcacc gtcgtcctgg gcgacgccgt cgcgcccgcg cgggagtgcc gcgtcagcgt
     2221 cacgctcggc gtcgccgtcc tgaagggcga gaagacggac gccgtggtgc gggacgcggt
     2281 gatgatgggc gtcgcggccc tccagccgat gctcacggac cggtctgacc ttccgccggc
     2341 cgccgtcgcg cgcgggacgc gcctcgaccg atggcgccgc atcgccgtcg cgtcggccaa
     2401 gcagtgcggg cgcgcagtcg tgcccgaggt gttcgccccg agttcgctgc cggaatgcct
     2461 ggcgcggcac cggcgcgacg cgggcgtcgt gctggtggaa ccctcgtcgg agggcctcgc
     2521 gcgggcccgc cggcccgtcc cgcgcgcgcc ggcctcggcg ctcgtgctcg tcggccccga
     2581 gggcgggtgg acccgcggcg aggtggatgc cgccgtcgcg tcggggtgcg cggccgtcac
     2641 gctcggcccg aggacccttc gggccgnnnn nnnnnngtgc ctgtcgtggc cctcggcgtc
     2701 ctctggcact actggggcga gctgatcgtg tagcgcgccg acccgccgcg gaccatggcg
     2761 gcgcaccacc cgtcttcctg ccgctgccag accgcctcgc cccacggcgc gaaggccgcg
     2821 agcacctgcg ggcgttcgtc cggagtgaag ccgctcacca ccagcgttcc cccgggggcg
     2881 accagcgccg tgagcgcgcc ggcggcggac atcagcaagg cccccgtcag gttcgcgagc
     2941 acgatgtccg ccggcgcaat ggcgcggctg cggaagtccg cttcctcgaa gcggacggcg
     3001 ggcaggcccg cgttgagctc ggcgttcccg cgggcgttct cgattgcgtc ggcatcgctg
     3061 tcgacgccga gggcctcgcg cgcgccgagg gcgcaggcgg cgaaggccag cacccccgaa
     3121 cccgtgccga cgtccagcac gcgccgtcgt c
//
LOCUS       DAOSIF010000374         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27069_length_3127_cov_4.394206, whole genome shotgun sequence.
ACCESSION   DAOSIF010000374 DAOSIF010000000
VERSION     DAOSIF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27069_length_3127_cov_4.394206"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..469
                     /locus_tag="PLT35_11260"
                     /pseudo
     CDS             129..469
                     /locus_tag="PLT35_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611458.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(676..1494)
                     /gene="istB"
                     /locus_tag="PLT35_11265"
     CDS             complement(676..1494)
                     /gene="istB"
                     /locus_tag="PLT35_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOG29846.1"
                     /translation="MTAAAPLSERVTTLLTTFGLTTAAQECVSRLTQAGHAAAWPVLH
                     DILDLERDARQHRRIDRLRRASRLPPGKTFDTLDVTRFPAPLMTRLHELADGHFLDTA
                     TNVLAFGLPGVGKSHAMAALGHALVQAGHAVLFVPAYALMQDLLAAKRALELPRALRK
                     LDLYEVVILDDLGYVQQSPEEAEVLFTLIAERYERRALAVTSNLVFSHWDRIFKDQMA
                     TAAAIDRLVHHAAILEFDVASYRTDHARRESLPAAPLRPKGRPPVSVLKRKRMR"
     gene            complement(1491..2972)
                     /gene="istA"
                     /locus_tag="PLT35_11270"
     CDS             complement(1491..2972)
                     /gene="istA"
                     /locus_tag="PLT35_11270"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOG29847.1"
                     /translation="MVTDAQVRRLREKRMGGKSLAAAAAAAGMSERAARTWQSGPLPS
                     ATRSPRTWRTRVDPFATVWATEIVPWLEADRDGRLQALTLFEELCTRHPGQFRPGQLR
                     TLQRRMRDWRALHGPDQEVFFEQAAVPGREGALDFTDPTDLGVTIQQQPFEHLWFEWI
                     LPFSGWTHIDLAPSETFEALVQGFQGAVWTLGAVPAIARHDNLSAATHELKRSGGRQL
                     TARFQAVLDHYGMRSSRIQPRQAHENGTVEQSHHQSKRAVAQALLLRGHADFADEDGY
                     LAFVRDVIQRKQNGPAAARLAEERIHLRPLPPAPIPNYTTYTCVVRRWSTIRVSGRTY
                     SVPSRLIGHTVEVRQAPATIEVRYGGEPVETMPRLRGRDHRIDYRHVIRSLVRKPGAF
                     ARYRYREELFPTLTFRQAYDALVTWHGERADVEYVRLLHLAATGSEGAVAQALTSLLS
                     ARRAFDYHVVQGLVAPVIPRVPVLRLPQPDLTVYDSLLAGGGA"
BASE COUNT          529 a         1048 c         1066 g          484 t
ORIGIN      
        1 cccatcggga tcaagacagt cggcttccgc atccaacagc gtatgcagcg tctctccctg
       61 acctgccggg cgatcttcgg accagttgaa acctgagagg atggctccca cagcccagtg
      121 aggagccgat gcgcaagagc cggttcagcg aagacgaggt ggtcaagatc ctccgcgagg
      181 cggataagac gccggtggcg gaggtcgcca agaagcacgg cgtggccgag cagacgatcg
      241 acatctggcg gcagcggttc gggaaactcg atcccgcgga cgtcaaacag ttgcgaccga
      301 tcgagcaaga gcacgggcgc ttgaagaaga tcgtcgccga acgggacgtc gaaatcgagc
      361 ttatgaaaga gatcgcccga acgaactggt aggcacgtcg gtgcggcgcc gccaggtcgc
      421 gtatgccaca tcacgcgggc tgtcgggccg acgtgcctgt gcgctgctcg ggaagctgca
      481 gtagccgggt tctcgaggct gccggatccg gctctgattg acgtcaatta cttttgccga
      541 tcagcgtcaa ctagtcttgc cggtcatgcc tcgtcaggtg cccgctggaa ttgcaggacc
      601 agcggacggt gagcgtcgtc gagcaggccc cccacccagg tgcggggccc gcgcagacga
      661 cgcggtgctg cgccgttatc tcatccgctt ccgtttcagc accgataccg gcggccgtcc
      721 cttcgggcgg agcggagcgg ccggcaggga ttcgcgccgc gcgtgatcgg tgcggtagct
      781 ggcgacgtcg aattctagga tggcggcgtg gtggaccaac cggtcaatgg cggcggccgt
      841 ggccatctgg tctttgaaga tccgatccca gtggctgaac acgagattgc tcgtgacggc
      901 cagcgcgcga cgctcatagc gctcggcgat cagcgtgaag agcacctccg cctcctcagg
      961 gctttgctgg acgtagccga gatcgtcgag gatgacgacc tcatacaggt ccagcttgcg
     1021 caaggcgcgc ggcagctcca acgcccgctt ggccgccagg aggtcctgca tcaaggcata
     1081 ggcgggcacg aagaggaccg cgtggccggc ctggacgagc gcgtggccca gtgcagccat
     1141 cgcgtggctc ttgccgacgc cgggcaaccc aaacgccagc acattcgtcg cggtgtcgag
     1201 gaagtggccg tcggccagct cgtgtagccg cgtcatcagc ggcgccggaa accgtgtgac
     1261 gtccaacgtg tcaaaggtct tgcccggggg gagccgcgag gcccggcgga gccggtcgat
     1321 gcggcggtgc tgccgggcgt cacgctcgag gtcgaggatg tcgtgaagga ccggccacgc
     1381 cgcggcgtgg ccggcctgcg tcaggcggct gacgcattcc tgggcggcgg tggtcagccc
     1441 gaacgtggtc aacagcgtcg tcacgcgctc actgagcggg gccgcggcgg tcatgcgcca
     1501 cctcccgcca gtaagctgtc gtagaccgtc agatccggct gcgggaggcg caggaccggc
     1561 acccggggga tgaccggagc cacgaggccc tgcacgacgt ggtagtcgaa ggctcggcgg
     1621 gcgctgagga ggctcgtcaa cgcctgggcc acggcgcctt cgctgcccgt ggccgccaga
     1681 tgcaacagcc ggacgtactc aacgtccgcg cgctcgccat gccacgttac caacgcgtcg
     1741 tacgcctgcc ggaaggtgag ggtgggaaag agctcctcgc ggtaccggta gcgggcaaac
     1801 gcccccggtt tgcggacgag gctgcgaatc acatggcggt agtcgatgcg atggtcacgt
     1861 ccccgcaacc gcggcatcgt ttcgaccggc tccccgccgt accgcacctc gatcgtcgcc
     1921 ggcgcttggc gcacctccac ggtgtggcca atcagccgtg acggcacgga gtaggtccgg
     1981 ccactcacgc ggatcgtgct ccagcgccgg acgacacagg tgtacgtcgt gtaattcgga
     2041 atgggggccg gcggcaacgg ccgcaagtgg atccgctcct ccgccaaccg cgccgccgcc
     2101 ggtccattct gcttgcgctg gatgacgtcg cggacgaagg ccaaatagcc gtcttcgtcg
     2161 gcgaagtcgg catgaccacg gagcagcagc gcctgcgcga ccgcgcgctt actctggtga
     2221 tgactctgct cgacggtccc gttttcgtgg gcctgccgcg gctgaatccg cgacgaccgc
     2281 atgccgtagt gatcgagcac cgcttgaaac cgggcggtca actggcgccc gcccgaccgc
     2341 ttcaattcat gcgtcgctgc cgagagattg tcgtgccggg cgatggccgg cacggccccc
     2401 agcgtccaga cggcgccttg gaagccctgc accaaggcct cgaaggtctc gctcggcgcc
     2461 aagtcaatgt gcgtccagcc gctgaagggc aggatccact cgaaccagag atgctcgaaa
     2521 ggctgctgct ggatggtgac cccgaggtcg gtgggatcgg tgaaatccaa ggcgccttcg
     2581 cgccccggca cggccgcttg ctcgaagaac acctcctgat cgggcccatg gagggcccgc
     2641 cagtcccgca tgcgccgctg cagcgtccgg agctggcccg ggcggaactg ccccgggtgg
     2701 cgggtgcaca actcttcaaa cagcgtgagc gcctggagtc gcccgtcgcg atccgcctcc
     2761 agccacggca cgatctccgt ggcccacacc gtggcgaacg ggtccacgcg agtccgccag
     2821 gtgcgcggcg acctcgtcgc cgacggcaac gggccagatt gccaggtgcg ggcggcccgc
     2881 tcgctcatgc cggcggctgc cgccgccgca gccagactct tgcctcccat ccgtttctcc
     2941 cgcagccgtc gcacctgtgc gtccgtgacc atccgtcctc cgacgaacag cctcgcacag
     3001 gccggatccg actgccgatg gaccggcaaa agtaattgtc gtcagaccgg caattctaat
     3061 tgtcgccaat cacggctcgg ctcgagggga gccggtgccg gacccggcat gaccccgctc
     3121 gccgcct
//
LOCUS       DAOSIF010000375         3118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27161_length_3118_cov_3.945805, whole genome shotgun sequence.
ACCESSION   DAOSIF010000375 DAOSIF010000000
VERSION     DAOSIF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27161_length_3118_cov_3.945805"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="truA"
                     /locus_tag="PLT35_11275"
     CDS             <1..768
                     /gene="truA"
                     /locus_tag="PLT35_11275"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG29848.1"
                     /translation="RLRRCRTGRRPRAVAAATGARRFELYGSGRTDAGVHALAQVAHL
                     QIDTRLTADALLSRINDELPGDIAVVALARAPRRFHARHDAVARSYLYQIARRRMAFA
                     KPYVWWVREDLDLDRMRAAAQRFVGLRDYRAFSADDREDAESRVLVDRVEVIDAPPLV
                     LVRVVGSHFLWRMVRRMVGVLVAVGRRDLRPDDVGRLLAGGAGEAAELTAPASGLFLE
                     RVFYTGAVPGGDARGDTGPDAASGGLSASGIPRSAFR"
     gene            1129..1281
                     /locus_tag="PLT35_11280"
     CDS             1129..1281
                     /locus_tag="PLT35_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29849.1"
                     /translation="MSRSREGVLDEHVHAVRGLWRERRDLRLAALDRESSGDVVWARP
                     EGDPIL"
     gene            1646..1798
                     /locus_tag="PLT35_11285"
     CDS             1646..1798
                     /locus_tag="PLT35_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29850.1"
                     /translation="MAGAPPSVALPQIAALPDARLGRYRSIVVGVIVGAILALTSLPA
                     VTLPLW"
     gene            complement(1914..>3118)
                     /locus_tag="PLT35_11290"
     CDS             complement(1914..>3118)
                     /locus_tag="PLT35_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG29851.1"
                     /translation="SVRGPAGWLVQVAVVTLLNVRTVRQRLGSSLVAVVGIAGVVTIF
                     VAVLSTAEGFRRTVQSAGSRDTAVVLRAGSDSEMVSVLSREDVSVIGDAAGLRRGAGG
                     AVVSGELFVIVDLLNRATNTTANVSLRGVGPNAFAVRPEVAIIQGRAFEAGRNELIVG
                     RGAAAQFAGTAVGRTLRFGRSDWTVVGIFDAGGTLADSELWCDAAVLQPLYQRGTTVQ
                     AVYAKLESPEAFAGFKDALTSDPRLNVKVMRETDYYAEQSQMITALISTIGVGIALLM
                     GIGAVFGAVNTMYTAVAARTREIAVLRALGFGGGPVAVSVLVESLLLAGAGGLAGGAI
                     AYAAFHGFRTSTMNWQSFSQVTFAFAVTPGIVARGVAYALAMGLAGGLLPAVRAARLP
                     IVSALREL"
BASE COUNT          419 a         1143 c         1125 g          431 t
ORIGIN      
        1 cggctccgca gatgccgcac cggtcgccgg cctcgcgccg tcgccgccgc gactggggcg
       61 cgccggttcg agctctacgg gtcgggccgc accgacgcgg gcgtacacgc cctcgcgcag
      121 gtggcgcacc tgcagatcga cacgcgcctc acggcggacg cgctcctctc gcgcatcaac
      181 gacgagctgc cgggcgacat cgccgtagtc gccctggcgc gtgcgccgcg gcgttttcac
      241 gcgcggcacg acgccgtcgc caggagctac ctttaccaga tcgcgcgccg gcggatggcg
      301 ttcgccaagc cctacgtgtg gtgggtccgg gaggatctcg atctggatcg catgcgggcg
      361 gccgcgcagc gcttcgtcgg cctccgggac taccgcgcct tctcggccga cgaccgcgag
      421 gacgcggagt cccgcgtgct ggtcgatcgc gtggaggtca tcgacgctcc gccgctcgtc
      481 ctcgtccgcg tggtgggctc gcatttcctg tggcggatgg tccggcggat ggtgggcgtc
      541 ctcgtggcgg tgggccgccg cgacctgcgc cccgacgacg tcgggcgcct gctcgccggc
      601 ggcgccggcg aggcggcgga gctgacggcc ccggcatcag gcttgttcct cgaacgggtg
      661 ttctatacag gcgccgttcc gggtggtgac gcacgcggag acacaggccc ggacgccgcg
      721 agcggcgggc tgagcgcatc agggatccca cgatctgcgt tccgctgaca gtcgtcgatc
      781 agcttgacag cgtctccctt cgcggccggt ctgccggcgc cggaagccct ccgggtgcca
      841 cggtgcaccg aggcagcctt cgccggcatg gcctgccgcg acccccgggg ccttcagcac
      901 gccaccgcgg acggctgccg gctcctgcgg aggccttcgc ggtggaggcc gctcgcgccg
      961 gcccggcgac ctggctcgaa cacgtgtttc tcgccagcag gtcccagccg cgcttcgacc
     1021 tccctgggaa gcgcgccgcc ggctgcgcaa ccgggtcatc gtcgaacggc ggcctgcgcc
     1081 gcggcggttc gggcctggcg agctgccagg cggcgttggt ccccgcctat gagccggtct
     1141 cgagaagggg tcctggacga gcacgtccat gccgtccgcg gcctgtggcg cgagcggcgc
     1201 gacctgaggc tcgcggcact cgaccgggag tcctctggag acgtcgtgtg ggccaggccg
     1261 gagggcgacc cgatcctctg actggcctgt ttgcccggag tggacggacg cacgcgtccg
     1321 gctggaggcc gccgcaacgc ggacggtcgt tatcgtcgcc ggccagccgc tcagcgccga
     1381 cgggtccggc cggacgaccc ggcggcggag cttctcgacc accgcgccgg cgcgcatcga
     1441 ggacagcgtt aggcgtccgg gcgcgtgcct ggaggaggcg acagccgcgc aaggctcgtc
     1501 accgcagtga cacgtttcgc gtgacaccgg tgtccgaaga ccggacaggg atcgccgccg
     1561 tcgcgcctgc cctgcggaaa acccggcgat accggacgag tgccgcgccc gtcttcctgg
     1621 cactggcttt gctatatccc ggggcatggc tggcgctccg ccgtccgtgg cgctcccgca
     1681 gatcgctgct ttgccagacg cgaggctggg aaggtatcga tccatcgtcg tcggcgtgat
     1741 cgtgggagcg atcctcgcgc tgacgtcgct gccggccgtg acgcttcccc tctggtagcc
     1801 cacgcgcgtg agacggggcc attccgtttc acgccgcggg gcgggactcc ccttccggtg
     1861 cggtcttcgc gcgtgtcgcc acgcgggcga gccggcgacc cgatccgctg gcgctagagt
     1921 tcccgcagcg ccgagacgat gggcagcctc gccgcgcgga cggccggcag caggccgccg
     1981 gcgagaccca ttgcgagcgc gtaggcgacg ccgcgtgcca cgatgcccgg cgtgacggcg
     2041 aaggcgaagg tcacctggct gaagctctgc cagttcatgg tcgacgtccg gaacccgtgg
     2101 aatgccgcgt aggcaatcgc gccgccggcc aggccgcccg ctcccgcgag gagcagcgac
     2161 tccacgagaa ccgagacggc gactgggccg ccgccgaagc cgagggcgcg cagcacggcg
     2221 atctcgcggg tgcgcgccgc cacggcggtg tacatcgtgt tgaccgcgcc gaacacggcc
     2281 ccgatgccca tcaggagcgc aatgccgacg ccgatcgtgg aaatgagcgc cgtgatcatc
     2341 tgcgactgct cggcgtagta gtccgtctcg cgcatgactt tgacgttgag ccgcggatcg
     2401 gaggtcagcg cgtccttgaa cccggcgaac gcctcgggag actcgagctt cgcgtacacg
     2461 gcctggactg tcgtcccgcg ctggtagagc ggctggagca cggcggcgtc gcaccagagc
     2521 tcgctgtccg ccagcgtccc tcccgcgtcg aagatgccca cgacggtcca gtcgctccgg
     2581 ccgaaccgca gcgtgcgacc gacggcggtc cccgcaaact gcgccgcggc cccccgcccg
     2641 acgatgagct cgttgcggcc ggcctcgaag gcgcgtccct ggatgatggc cacctcggga
     2701 cggacggcga acgcgttggg gccgacgccg cgcagcgaga cgttggccgt cgtgttcgtg
     2761 gcgcggttca gtaggtccac gatgacaaac agctcgcccg acaccaccgc gccgccggcg
     2821 ccgcggcgca ggcccgccgc gtcgccgatg acgctcacgt cctcgcgcga cagcacgctc
     2881 accatctcgc tgtccgaacc ggcgcgcagg acgacagccg tatcgcgcga cccggccgac
     2941 tgcaccgtcc gccggaagcc ctcggcggtc gagaggacgg cgacgaagat ggtgaccacg
     3001 cccgcgatgc cgacaacggc cacgagcgag gagccgagcc gctggcggac cgtccgcacg
     3061 ttgagcagcg tcacgacggc gacctgcacg agccagccgg cggggccgcg cacgctca
//
LOCUS       DAOSIF010000376         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27212_length_3113_cov_4.646174, whole genome shotgun sequence.
ACCESSION   DAOSIF010000376 DAOSIF010000000
VERSION     DAOSIF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27212_length_3113_cov_4.646174"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..843
                     /locus_tag="PLT35_11295"
     CDS             79..843
                     /locus_tag="PLT35_11295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG29852.1"
                     /translation="MARLQGRSAIVTGATSGMGRATALLFAREGAAVVASGRDEERGR
                     ALVGQIRAAGGRAEFVPGDVSLPDVNARLVDACTGRFGGLDTIVCFAGMLGLGSITGV
                     PADTWRRTLAVNLDAVFYLLRAGLPVMQAGGGGSVVIGGSIAAMKGFPNHAAYCASKG
                     ALLALVRQAAIDYGPDVRFNVLCPGPVDTPLIWDSAAAFPDPARAVSDVAERTLLKRL
                     GTPEDIARAALFLASNESGFVTGTSLTIDGGIMTGA"
     gene            916..>3113
                     /locus_tag="PLT35_11300"
     CDS             916..>3113
                     /locus_tag="PLT35_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOG29853.1"
                     /translation="MITRITRLAAAPAALAIALAAFGATERATQAQARITSPEQFFGF
                     RMGADRKLVRWDKAVEYYRLLEKQSGGKLKVVDMGPTEMGNPFLLVVITSPANQARLE
                     ELRRMNLTLSDPRGVPEAEVDRIVAEGKAVVCQTMSMHANEVGGINMAPELTYDLVSR
                     TDEETRRILDNVIYLEIPSFNPDGAIMIHDYYAKYLGTEYEGGPLPWLYHKYIGHDNN
                     RDALTMNMKESRYVGRLMFVDWKPQAYVDHHQMGAYGARIYVPPYAEPVRPLADPQVW
                     REMQWYGAHIAYKEEEAGLSGVMNAGQYSGWGHFGFHWITPFHNIAGMLTESASVRVA
                     TPIFIHPSQLEGGARDFETYQEQMNFPNPWPGGWWTLRDIVERQKVSAWATLDLAARN
                     RETVLRNAYLKASRQTERGAKGRPAAYAMTARQHDPLTLNKLVNALLDHGIEVQQSPK
                     GFAAPDGMTYPPGSFYVPLAQPKMGLIRYLLGETHYPDNEHTRRQDGTPIRPYDMAQD
                     VLAEFMGIRVDPLDEAVRGEFVKVAAPIAPAGTVAPQARLGYVLDGRLNDSFRAVNLL
                     WDRGVTVRRVDAANGALRPGDWLVPAGSEPVLAEAARLTGVDFRRLEADVAGGSHTTG
                     RLRIGIYRRYRGGNMDEGWTRWLFEQWGFPFAEVYDAEIQQGDLNAKYDVLVFADDST
                     AAITGEPAEGGRGGGRGGGTFSSVPNRTPPEYRSGIGAKGVEAIRDFVQKG"
BASE COUNT          482 a         1118 c         1096 g          417 t
ORIGIN      
        1 tcccgcagag acaccctcgc gggcttccgc gcggccctgg cccgccccgc ccccggccgc
       61 tgtagactac tccccaccat ggcgcgactg cagggccgtt cggccatcgt caccggggcc
      121 accagcggga tgggccgcgc gacggccctt ctcttcgcgc gcgagggcgc ggccgtcgtc
      181 gccagcggcc gcgacgagga gcggggccgg gcgctggtcg gccagatccg cgctgcgggc
      241 ggccgcgcgg agttcgtgcc cggcgatgtc agcctcccgg atgtcaatgc gcggctcgtg
      301 gacgcgtgca cgggccgctt cggcggcctc gacaccatcg tctgtttcgc cggcatgctc
      361 ggactgggct ccatcaccgg tgtgccggcc gacacgtggc gccggaccct cgccgtgaac
      421 ctggacgccg tcttctacct gcttcgtgcg ggcctgccgg tgatgcaggc cgggggcggc
      481 gggtcggtcg tcatcggcgg gtcgattgcg gcgatgaagg ggttcccgaa ccacgccgcc
      541 tactgcgcgt ccaagggcgc gctgctggcg ctcgtccgcc aggcggccat cgactacggc
      601 ccggacgtcc gcttcaacgt cctctgccca ggccccgtcg acaccccgct catctgggac
      661 tcggccgcgg cgttcccgga cccggcgcgc gccgtctccg acgtcgccga gcgaacgctc
      721 ctgaagcggc tcgggacgcc cgaggacatc gcgcgcgcgg cgctcttcct cgcgtcgaac
      781 gaatcgggct tcgtgacggg cacctcgctc acgatcgacg gcggcatcat gaccggcgcc
      841 tgacgccggc ccgctcggcg cgcgccgcct gtgatatcct cgcgccgcat cgagcctcct
      901 ccgagggaga tccacatgat cacacgcatc acgcggctgg ccgcggcgcc ggcggccctc
      961 gccatcgcgc tcgccgcctt cggcgccacc gagcgcgcga cgcaggccca ggcgcgcatc
     1021 acctcgccgg agcagttctt cggcttccgg atgggcgccg accgcaagct cgtgcgctgg
     1081 gacaaggcgg tcgagtacta ccggctcctc gagaagcaga gcggcggcaa gctgaaggtc
     1141 gtggacatgg ggcccaccga gatgggcaac ccgttcctcc tcgtcgtcat cacgtcgccc
     1201 gccaaccagg cgcggctcga ggagctgagg cggatgaacc tcacgctcag cgacccccgc
     1261 ggcgtgccgg aggcggaggt cgaccggatc gtcgccgagg gcaaggccgt cgtctgccag
     1321 acgatgagca tgcacgcgaa cgaagtgggc ggcatcaaca tggcgcccga actgacctac
     1381 gacctggtgt cccgcaccga cgaggagacg cggcggatcc tcgacaacgt catctacctg
     1441 gagatcccgt cgttcaaccc cgacggcgcg atcatgatcc acgactacta cgcgaagtac
     1501 ctggggacgg agtacgaggg cggcccgctg ccgtggctct accacaagta catcgggcac
     1561 gacaacaacc gcgacgccct gacgatgaac atgaaggagt cgcggtacgt cggccggctg
     1621 atgttcgtgg actggaagcc gcaggcctac gtggatcacc accagatggg cgcctacggg
     1681 gcgcggatct acgtcccgcc ctacgccgag cccgtccgcc cactggcgga tccccaggtg
     1741 tggcgcgaga tgcagtggta cggcgcccac atcgcgtaca aggaggagga ggccgggctc
     1801 tcgggcgtga tgaacgcggg ccagtactcc ggctggggcc acttcggctt ccactggatc
     1861 acgccgttcc acaacatcgc cggcatgctg accgaatcgg cgagcgtgcg cgtcgcgacg
     1921 cccatcttca tccacccgag ccagctcgag ggcggcgcgc gggacttcga gacctaccag
     1981 gagcagatga acttcccgaa cccctggccc ggcggctggt ggacgctcag ggacatcgtc
     2041 gagcgccaga aggtgtcggc gtgggccacg ctggatctgg cggcgcgcaa ccgcgagacc
     2101 gtgctgcgca acgcctacct gaaagcgagc cggcagaccg agcggggggc gaagggcagg
     2161 ccggcggcct acgcgatgac cgcgcggcag cacgatccgc tgacgctgaa caaactggtc
     2221 aacgccctgc tcgaccacgg gatcgaggtg cagcagtccc cgaaagggtt cgccgcgccc
     2281 gacggcatga cgtacccgcc gggctccttc tacgtcccgc tggcacagcc gaagatggga
     2341 ctgatccgct acctgctcgg cgagacccac tacccggaca acgagcacac ccggcggcag
     2401 gacggcacgc ccatccggcc ctacgacatg gcgcaggacg tgctggccga gttcatgggc
     2461 atccgcgtgg acccgctgga cgaggccgtc cgcggcgagt tcgtgaaggt cgccgctccc
     2521 atcgcgccgg ccggcacggt ggcgccgcag gcgcggctgg gatacgtcct ggacggccgg
     2581 ctcaacgaca gcttccgcgc ggtcaatctg ctctgggacc ggggcgtcac cgtcaggcgc
     2641 gttgacgcgg ccaacggagc gctccggccc ggcgactggc tcgtgccggc cggatcggag
     2701 cccgtgctgg ccgaggccgc gcggctgacg ggcgtggact tcaggcgcct cgaggcggac
     2761 gtggcgggcg gcagccatac gacgggccgc ctgcgcatcg gcatctaccg ccgctaccgc
     2821 ggcggcaaca tggacgaggg ctggacccgg tggctcttcg agcagtgggg cttccccttc
     2881 gccgaggtct acgacgcgga gatccagcag ggcgacctga acgcgaagta cgacgtgctc
     2941 gtcttcgccg acgactcgac tgcggcgatc accggcgagc cggccgaggg cgggcgcggc
     3001 ggcggccgcg gcggcggaac cttctcgagc gtcccgaaca ggacgccgcc ggagtaccgg
     3061 agcgggatcg gcgcgaaagg cgtcgaggcg atcagggatt tcgtccagaa ggg
//
LOCUS       DAOSIF010000377         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27243_length_3111_cov_4.208442, whole genome shotgun sequence.
ACCESSION   DAOSIF010000377 DAOSIF010000000
VERSION     DAOSIF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27243_length_3111_cov_4.208442"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1801
                     /locus_tag="PLT35_11305"
     CDS             239..1801
                     /locus_tag="PLT35_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG29854.1"
                     /translation="MPADTGQPGWRVDVRRTLTRNTAWNYTGFAVNLLTNLLLFPYVV
                     REIGDSAAGIWLLLGSVTGYMGLLELGLVPALSQSVAVCRARGDTAGLNRSASTAITL
                     LTLLAAIPLMLVAAVPWMVEALRVPPALTGQARAVFAIAIAGFAARMPLAAFQAVLLG
                     TQRQDRTNQLWILIVLAKFVLAVLLLWLGFRLVAIVTMEALVHLAAGLLQYRWIRQEV
                     PGLAVRVGALDRPEARALVGFGSSLLVGSLCSLLIEQSDKIVISAFLPISMVTYYAAA
                     WKLYAFAYTLPTTLVQAVAPLAGDLHGRGDREKLRQLYLRMTKYTVAVAWPLVLSLGF
                     CGALVLDVWMGPEFARHAAILQVLLVAFAVTSHNHVGYSVLLGMRRIAPIIWWYKVPQ
                     ALLNVALSVWLVTELGILGVALGTMIPAVALEFVWLHLVKRELSVGWGAFAREVVVPA
                     AAPAAVAFAPLALAYWRIDHRSALLLVAAAACSLLYAVLFWTRSLSKAERSELLAHVP
                     QRHRIPFLTTSP"
     gene            1814..2959
                     /locus_tag="PLT35_11310"
     CDS             1814..2959
                     /locus_tag="PLT35_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG29855.1"
                     /translation="MRIGFDARYLSRGLVGGVRTYVFHLARTLPGLAPDDEFVYYIDA
                     KAPFEVERCPSNVSLRTLPWISPLSTLANDMRIARWMARDLVDVAHFPGNYGPRGAYA
                     LVVTVHDALNLFRMSEHRRGFGRKPRQVAMMAYLGWQTRRALADADSVLTTSEHARGE
                     IARLGGRRPDRIVAIHEAAGEEFTPVTDPAALESARACFQRRPLMVLADGIKNPEALI
                     EAYGLLPDAIRARTEIVFFSRETGPRPPVAAAMADPAVRFLARPSTADLALLMNLAAV
                     FVFPSWYEGFGIPLVEAMSCGAPVVASTRGAIPEVVAGAGLLFDVERPAELARHLERV
                     LESEAVRSDLRARSLARAKAFSWHTTARRTLEAYRAAVEARLSAERP"
BASE COUNT          398 a         1118 c         1068 g          527 t
ORIGIN      
        1 gccaggggcg ccgcctgagg gtgtccgcca tggcgcccac gatctcgcgg ctgcgatcgc
       61 cgccgggccg acccaggacc ccgaagattc cgctcaccga acctccacgg agcagcgcgc
      121 ctggctggcg tctcggaacc ggccggcgtg agactatcat acctatcgcc ggccggccgc
      181 tggccgaatc cagtggtacc cggctggcgc agcgcaacga tctcgagaag ggccgcagat
      241 gcctgctgac accggacagc ccggctggcg cgtggacgtc cgcaggacgc tgacgcgcaa
      301 caccgcctgg aactacaccg ggttcgcggt caacctgctc acgaacctgc tgctcttccc
      361 ctacgtggtc cgcgagattg gcgactccgc cgccgggatc tggctgctgc tcggctcggt
      421 caccggctac atggggctgc tcgaactcgg gctcgtcccg gccctgtcgc agtcggtcgc
      481 cgtttgccgc gcccgcggcg acaccgccgg cctgaaccgc tcggcctcga cggctatcac
      541 gctgctcacg ctcctggcgg cgatccccct gatgctggtc gcggccgttc cctggatggt
      601 cgaggcgctg agagtgcctc cggcccttac cggacaggcc cgcgcggtgt ttgcgatcgc
      661 catcgcgggc ttcgcggcgc gcatgcccct ggcggccttc caggccgtcc tgctcggcac
      721 ccagcggcag gaccgcacga accagctctg gatcctgatc gtgctggcga agttcgtgct
      781 ggccgtgctg ctcctctggc tgggctttcg cctcgtcgcc atcgtgacga tggaggcgct
      841 ggtccacctc gccgccgggc tgctgcaata ccggtggatt cggcaggaag tgccggggtt
      901 ggccgtgcgc gtcggagcgc tcgacaggcc ggaggcccgc gcgctcgtcg gcttcggcag
      961 ctcgctcctg gtcgggtccc tgtgcagcct gctgatcgag cagagcgaca agatcgtgat
     1021 cagcgcgttc ctgccgatct cgatggtgac ctactacgcg gcggcgtgga agctgtacgc
     1081 cttcgcgtac actctgccca cgacgctcgt ccaggctgtg gcgcccctgg ccggcgatct
     1141 ccacggacga ggcgaccggg agaagctccg tcagctctac cttcggatga cgaagtacac
     1201 cgtcgccgtc gcgtggccgc tcgtcctctc gctcggcttc tgcggcgcgc tcgtgctcga
     1261 cgtctggatg gggccggagt tcgcccgcca cgccgccatc ctgcaggtcc tgctcgtggc
     1321 gttcgcggtg accagccaca accacgtcgg ctactccgtg ctcctgggca tgcggcgaat
     1381 cgccccgatc atctggtggt acaaggtgcc gcaggccctc ttgaacgtgg cgctcagcgt
     1441 ctggctcgtg actgagctgg gcatcctcgg cgtcgcgctc ggcaccatga tcccggccgt
     1501 cgccctcgag ttcgtctggc tgcacctcgt gaagcgcgag ctgtcggtgg ggtggggcgc
     1561 gttcgcgcgc gaggtcgtcg tgccagcggc ggcgccggcg gccgtggcgt tcgcgcccct
     1621 ggccctcgcc tactggcgaa tcgatcaccg gtcagcgctg ctgctggtcg ccgcggctgc
     1681 gtgcagcctc ctgtacgcgg tgctcttctg gacgcgctcg ctctcgaagg cggagcgatc
     1741 ggagctcctc gctcacgttc cccagcggca ccggattccc ttcctgacga cgtcgccttg
     1801 agggaaggtc gggatgcgca tcggcttcga cgcccggtac ctttcgcgcg gcctggtcgg
     1861 gggggtccgc acgtacgtgt tccacctggc gcgaacgctt ccggggctgg cccccgacga
     1921 cgagttcgtc tactacatcg acgccaaggc gccgttcgaa gtggagcgtt gtccgtcgaa
     1981 tgtctcgctg cggacgctcc catggatctc tccgctgagc accctggcga acgacatgcg
     2041 aatcgcgcgc tggatggcga gggacctggt ggacgtcgct cacttccccg gcaactacgg
     2101 gccgcgcggg gcctacgcgc tcgtcgtgac ggtgcacgat gcgctgaacc tgttccgcat
     2161 gtccgagcac cgccgcggct tcggcagaaa gccccgccag gtcgcgatga tggcgtacct
     2221 gggctggcag acgcggaggg ccctggccga cgccgattcg gtcctgacca ccagcgagca
     2281 tgcgcggggc gagatcgcgc ggctgggcgg gcggcggccc gaccgaatcg tggccattca
     2341 cgaggccgcg ggcgaggagt tcacgcccgt cacggacccg gccgccctcg agtccgcccg
     2401 cgcgtgcttc cagcggcggc cgctgatggt ccttgccgac ggcatcaaga accccgaggc
     2461 gctgatcgag gcgtacggct tgcttccgga cgcgatccgc gcgcgaaccg agatcgtctt
     2521 cttcagccgc gagacggggc cccgcccacc ggtcgccgcc gcgatggccg atccggccgt
     2581 gcgcttcctg gcgcgcccgt ccaccgccga cctcgcgttg ctgatgaacc tggccgccgt
     2641 cttcgtgttt ccctcgtggt acgagggctt cggcatcccg ctcgtcgaag cgatgagctg
     2701 cggcgcgccg gtcgtcgcgt cgacgcgggg cgcgatcccc gaggtggtcg caggcgccgg
     2761 cttgctcttc gacgtcgagc gtccggcgga actcgcccgc catctcgagc gggtcctgga
     2821 gtcggaggct gtcagatcgg acttgagggc ccggagcctt gcccgcgcga aggccttttc
     2881 gtggcacacg accgccagga ggacgctcga agcctaccgg gcggccgtcg aagcgagact
     2941 gtcggctgaa cggccatgaa catcctgtac gtcagcgatt cgaccacgct cagcggagcc
     3001 gagatcgtgc tgctgggcta cctggatcgc cgtgcgccgc cggaccgccg ggctcgcgta
     3061 tacctgcacc ggctgaaccg gcgcctcgcc gacgcgctgg acgagcgggg c
//
LOCUS       DAOSIF010000378         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27340_length_3103_cov_4.152559, whole genome shotgun sequence.
ACCESSION   DAOSIF010000378 DAOSIF010000000
VERSION     DAOSIF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27340_length_3103_cov_4.152559"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLT35_11315"
     CDS             <1..275
                     /locus_tag="PLT35_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29856.1"
                     /translation="FSYTIVFAPFDGPGYARSLELARASSDYLEVGHGGSLRHRARFT
                     PEQVLALRDLWAIVGGLDTTEVLVDDRPLPFARELWLPLLWYLLPR"
     gene            275..865
                     /locus_tag="PLT35_11320"
     CDS             275..865
                     /locus_tag="PLT35_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HOG29857.1"
                     /translation="MARGSDLERSLQKLEADLRRLEAEYNMFFAGQLPRPPWETRTQV
                     EQSVAKLERASAIEGSYAERFRLQALMSRLATFVDLWDRGLRAREEGRPGPFAHRGQP
                     AAGAASQGDRVVYVTVFADPVRESDRLRDLYERVSDVRRELGERQVPFHRFAGLIRDK
                     VAVLRKRGAPEVAFRVTIHDGKLTFSARGLKGSRGE"
     gene            complement(1019..1222)
                     /locus_tag="PLT35_11325"
     CDS             complement(1019..1222)
                     /locus_tag="PLT35_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOG29858.1"
                     /translation="MRITGKVKWFNNAKGFGFIEREGGSDVFVHYSAITGNGFRSLEE
                     GQAVEFEIVDGPKGPQAGNVIKI"
     gene            1470..3071
                     /gene="purH"
                     /locus_tag="PLT35_11330"
     CDS             1470..3071
                     /gene="purH"
                     /locus_tag="PLT35_11330"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOG29859.1"
                     /translation="MRALISVSNKAGLVEFARGLVQRGWDIVSTGGTARALADAGVPV
                     VQVAEVTGAPEMMDGRVKTLHPMIHGGILARRDHASDREAMERFRITPIDMVVVNLYP
                     FCETAARPGTAFEALVEDIDIGGPSMIRAAAKNFKDVLVVTSPGDYGRVLAALDEPGG
                     PSMELRFALAKEAFTHTGSYDCAIANELEAVAVEGGACRRLAERDPLPPRLIVATNRH
                     RTLRYGENPHQPGAWYVPADVAPGDLPAVLQGKELSFTNLLDVDAASRIAAEFVEPAA
                     VVIKHTNPCGAATGLDLAEAYVRARDADPLSAFGGIVGCNREVDEATAQAIASTFIEA
                     VIAPAITEAALAVLAARKNLRVVIAPAAFGAGGVSRTLGRLDLRSALGGILAQDADRV
                     AEARDEWPREGGPEVVTRRAPTPAEWKALRFAWRVCAHVKSNTVIFTSEDRTLAVGAG
                     QMSRVDAVNVARMKAEAAKLSLAGSVAASDAFFPFRDGLDAIVAAGATAVVQPGGSVR
                     DADVIAAADEHGIAMVLTRRRHFRH"
BASE COUNT          429 a         1099 c         1132 g          443 t
ORIGIN      
        1 cgttttccta cacgatcgtg ttcgcgccct tcgacggccc cggctacgcg cggtcgctgg
       61 aactcgcccg cgcgagtagc gactacctcg aagtcggcca cggcggcagc ctgcgccacc
      121 gggcccgttt caccccggag caggtgctgg cgctccgcga cctgtgggcg atcgtcggcg
      181 gcctcgacac gaccgaggtg ctcgtcgacg accggccgct cccgttcgcg cgcgagctct
      241 ggctgccgct cctctggtat cttctcccgc gctagtggcc cgcggatccg atctcgaacg
      301 cagcctgcag aagctcgagg ccgacctgcg caggctcgag gccgagtaca acatgttctt
      361 cgccgggcag ctccctcgcc cgccgtggga gacgcgcacg caggtcgagc agtcggtggc
      421 gaagctcgag cgcgcgagcg cgatcgaggg ctcgtacgcg gagcggttcc gcctgcaggc
      481 gctgatgtcg cggctggcga ccttcgtgga tctgtgggac cgggggctgc gggcccgcga
      541 ggagggacgc ccggggccgt tcgcccaccg cggccagccc gcggcgggcg cggcgtcgca
      601 gggcgatcgc gtcgtctacg tcacggtgtt cgccgacccg gtccgcgagt cggaccggct
      661 ccgcgatctc tacgagcgcg tcagcgacgt gcgccgcgaa ctcggcgagc ggcaggtgcc
      721 gttccacagg tttgccggcc tgatccgcga caaggtggcg gtcctcagga agcgcggcgc
      781 gcccgaggtg gcgttccgcg tgacgatcca cgacggcaag ctcaccttca gcgcgagggg
      841 cctcaagggc tcgcgcggcg agtgagcggc aggacagccg cgccagccgc ggcggcctgc
      901 gggcagggcc cggccggcct cctcctcatc tcgcgccgtc gggttgtcgg ggaatcgcgc
      961 ctgaaaaaag accggggcgg ctgggccgcc ccggtaaagc tgtcggaagg ggtagtcgct
     1021 agatcttgat gacgttgccc gcctgaggcc ccttcgggcc gtcgacgatc tcgaactcca
     1081 cggcctggcc ctcctcgagc gaacggaagc cgttgcccgt gatggccgag tagtgaacga
     1141 acacgtcgct cccgccctcg cgctcgatga atccgaaccc cttcgcgttg ttgaaccact
     1201 tcaccttgcc tgtaatgcgc atacgtgccc tgccttgcct tgtcgcggcg ccgggcgcgc
     1261 cgcggtcttg ccgctccccg gctccagccg gagaaagtcc cgtcgatccg ctgttcgtgc
     1321 ggcgggcgcc cgcccggagg cacccgatcg gcatgttacc ggccgcgttt gcggagtgtc
     1381 aagacgagcg gacggcgagc ggacggcaag cgacgcggct atactctcta ctctctggca
     1441 gccgtcatcg cctcccggga gtgagcatca tgcgcgcatt gatcagcgtg tcgaacaagg
     1501 ccggcctcgt ggagttcgcc cgcggcctgg tccagcgggg ctgggacatc gtctcgacag
     1561 gaggcaccgc gcgcgcgctg gcggacgccg gcgtgccggt cgtccaggtg gccgaggtga
     1621 cgggcgcgcc cgagatgatg gacggccgcg tcaagacgct gcacccgatg atccacggcg
     1681 gcatcctcgc ccgccgcgac cacgcgagcg accgggaggc gatggagcgg ttccgcatca
     1741 cgccgatcga catggtcgtc gtgaacctct acccgttttg cgagacggcc gcgaggccgg
     1801 gcacggcgtt cgaggcgctc gtcgaggaca tcgacatcgg cgggccgtcg atgatccggg
     1861 cggccgccaa gaacttcaag gacgtgctcg tggtgacgtc gccgggcgac tacggccgcg
     1921 tgctggccgc cctcgacgag ccgggcgggc cgtcgatgga gctgcgcttc gccctggcga
     1981 aggaggcgtt cacccacacg gggagctacg actgcgccat cgccaacgag ctggaggccg
     2041 tcgccgtcga gggcggcgcg tgccggcgcc tggccgagcg cgacccgctg ccgccgcgtc
     2101 tgatcgtcgc cacgaaccgc caccgcacgc tgcgctacgg cgagaacccg caccagcccg
     2161 gcgcgtggta cgtgcccgcc gacgtggcgc ccggcgacct gcccgccgtg ctgcagggca
     2221 aggaactgtc cttcaccaac ctgctggacg tggacgccgc gtcgcgcatc gccgccgagt
     2281 tcgtcgagcc cgccgcggtc gtcatcaagc acacgaaccc gtgcggcgcc gcgacgggcc
     2341 tcgacctggc cgaggcctac gtccgcgcgc gcgacgccga tccgctgtcc gccttcggcg
     2401 gcatcgtcgg gtgcaaccgc gaagtggacg aggcgacggc gcaggccatc gcctccacct
     2461 tcatcgaggc cgtgatcgcg ccggcgatta ccgaggcggc gctggcggtc ctcgcagcca
     2521 ggaagaacct gcgcgtggtc atcgcgccgg cggccttcgg cgccggcggt gtcagccgga
     2581 ccctgggccg cctcgacctc cggtccgcgc tgggaggcat cctcgcgcag gacgcggacc
     2641 gcgtggcaga ggcgcgggac gagtggccgc gcgaaggcgg gccggaggtc gtgaccaggc
     2701 gggcgccgac gccggccgag tggaaggccc tgcggttcgc gtggcgggtc tgcgcgcacg
     2761 tgaagtcgaa cacggtgatt ttcacgtccg aggaccgcac gctcgcggtc ggcgccggcc
     2821 agatgagccg ggtcgacgcg gtgaacgtgg cccggatgaa ggccgaggcg gcgaagctgt
     2881 cacttgcggg gtcggtggcg gcctcggacg ccttcttccc gttccgcgac ggcctcgatg
     2941 cgatcgtggc cgccggggcg acggccgtcg tgcagccggg cggatcggtg cgcgacgccg
     3001 acgtcatcgc ggcggccgac gagcacggca tcgcgatggt cctgacgcgc cgccggcact
     3061 tcagacactg atggagtaat ggggtcagat ccagcaaaac ggg
//
LOCUS       DAOSIF010000379         3094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27444_length_3094_cov_3.489306, whole genome shotgun sequence.
ACCESSION   DAOSIF010000379 DAOSIF010000000
VERSION     DAOSIF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27444_length_3094_cov_3.489306"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLT35_11335"
     CDS             complement(<1..298)
                     /locus_tag="PLT35_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29860.1"
                     /translation="MDATAHETFPRVFWSANVTELFERAAYYSMASFVVIYLGQLGFG
                     DYWPSTLNGLLWTLVYFLPILSGTIADQIGFRRALLAAFVLLACGYLLMGYPVWL"
     gene            427..>3094
                     /locus_tag="PLT35_11340"
     CDS             427..>3094
                     /locus_tag="PLT35_11340"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOG29861.1"
                     /translation="MRRDARPQLAFILVVFALGILWPSAAGYYTDWLWFGETGYQQVY
                     LTTLATRFGLGIVVGLLVFGALFANYQLAFQHAGEPYVVLGLSPADGTPILLQRRGLS
                     RLIAAVSALAGLAAGVFASSRWMSWLQYRHATAFGERDPIFGQDVAFYVFSLPWLGAV
                     RDVVLVTALLALAGSALIYLMPGRSTALAREGPFAALRSGRRHLALIAAAVLLLLAAG
                     AWLDAFGLLLSPAGLIQGATYADVTIRLPALRLLTVAGAAGAVLAVAHAFTRRSWMLA
                     LAFLAYAVIWAGGAIASTAFQRLVVTPNEQVKETPYLAHNIAATRKAFALDRVEERDL
                     SGDSLLSRADIDANEATLGNVRLWDSQPLLDTFGQMQEIRTYYDFTSVDIDRYVIDGR
                     YRQVMLSARELNSESLPAKSWINEHLTFTHGYGLTLGPVNEVTEEGLPVLFIRNIPPE
                     SSVDLEVTEPSIYYGELSNDHVFVGTRAREFHYPRGDDNVVKNYTGRGGVPVGSLIRK
                     LAFASRFRSLKILLSEDLGPESRILFRRRILDRAAAIAPFLTFDRDPYLVIADGRLFW
                     MLDAYTLTGQYPYSTRTEGGFNYIRNSVKVVIDAHNGTTTFYLWEPADPLAVTIGRVF
                     PGLLRPMAELPEPLRRHVRYPEGIFSVQSRMYATYHMTNPSVFYNKEDQWEVPSIESG
                     RTSAPMEPYYTIMRLPQEREAEFVQMLPFTPRRKDNLAAWMVARSDGSHYGRMLVFQF
                     PKQKVVFGPRQIVARINQDQVIAPQITLWNQQGSEVIQGTLLVIPLEESLLYVRPLYL
                     RAAGGKIPELKRVIVAHQNLIVMEESLDAALDRIFRKGGPARPAAADAASNGAPALSA
                     AAPLQAEEPLEALAGAARQHFERAIQAQRDGDW"
BASE COUNT          460 a         1081 c         1063 g          490 t
ORIGIN      
        1 cgagccacac gggatacccc atcagcaggt agccgcaggc gagcaggacg aaggcggcga
       61 gcagcgcccg ccggaacccg atctgatcgg cgatggtgcc tgacaggatt gggaggaagt
      121 acacgagcgt ccacaagagc ccgttgaggg tcgaaggcca gtagtcgccg aacccgagct
      181 ggccgaggta gatcacgacg aagctcgcca tcgagtagta ggcggcgcgc tcgaagagct
      241 cggtgacgtt ggcgctccag aagacccgtg ggaacgtctc gtgggcagtg gcatccatgt
      301 gggtggctcc cgtcagtccc ggcggcggcg cccggacgcg ctcgccgcgc atgataccgc
      361 atgggcccgg ggtgcgcggc cgcgaaaact gcggtacagt ggctgggtcc tcgtggaggc
      421 accggcatga ggcgtgacgc ccgcccgcag ctcgcattca tcctggtcgt cttcgccctc
      481 ggcatcctgt ggccgtcggc ggcgggctac tacaccgact ggctgtggtt cggcgagacc
      541 ggctaccagc aggtgtacct gaccacgctc gcgacgcgat tcggcctggg catcgtggtc
      601 ggcctcctgg tcttcggcgc gctgttcgcc aactatcagc tggcgttcca gcacgccggc
      661 gagccctatg tcgtgctggg cttgtcgccg gccgacggca cgccaatcct cctccagcgg
      721 cgcgggctct cgcgcctgat tgccgccgtg tcggcgcttg ccgggctggc cgccggcgtc
      781 ttcgcgtcga gccgctggat gagctggctg cagtaccgcc acgcgaccgc cttcggcgaa
      841 cgcgacccga tcttcgggca ggacgtggcc ttctacgtgt tcagcctgcc ctggctcggc
      901 gccgtccgcg acgtcgtgct cgtcacggcg ctgctggcgc tggcgggctc ggcgctgatc
      961 tacctgatgc ccggccggtc gaccgccttg gcgcgcgaag gccccttcgc ggcgctgcgg
     1021 agcgggcgcc gccacctcgc gctgatcgcg gcggcggtgc tgctactgct ggcggccggg
     1081 gcgtggctgg acgccttcgg gctcctgctc tcccccgccg gcctcatcca gggcgccacc
     1141 tacgccgacg tcacgatccg gctgccggcg ctccgcctgc tcacggtcgc aggcgcggcc
     1201 ggggcggtgc tcgccgtggc gcacgccttc acgcgccgct cttggatgct cgcgctggcc
     1261 tttctggcct acgcggtgat ctgggcgggc ggcgccatcg cctccacggc gttccagcgg
     1321 ctcgtcgtca cgccgaacga gcaggtgaag gagacgccct acctggccca caacatcgcg
     1381 gcgacccgca aggcgttcgc gctggaccgc gtcgaggagc gcgatctgtc gggcgactcg
     1441 ctgctgtccc gggcggacat cgacgccaac gaggcgacgc tcggcaacgt gcgcctgtgg
     1501 gacagccagc cgctgctcga caccttcggc cagatgcagg agatccggac gtactacgac
     1561 ttcacgtccg tcgacatcga ccgctacgtg atcgacggcc gctaccgcca ggtcatgctc
     1621 tcggcgcggg aactgaactc ggagagcctg ccggccaagt cctggatcaa cgagcacctg
     1681 acgttcacgc acgggtacgg cctcacgctc gggccggtca acgaggtgac ggaggaaggc
     1741 ctgccggtcc tgttcatccg gaacatcccg cccgagtcgt cggtggacct ggaggtgacc
     1801 gagccgtcca tctactacgg cgagctctcg aacgaccacg ttttcgtcgg cacccgcgcc
     1861 cgcgagttcc actatccgcg aggcgacgac aacgtcgtca agaactacac cggccgcggc
     1921 ggcgtgccgg tgggatcgct catccggaag ctggcgttcg cgagccggtt ccgttcgctc
     1981 aagatcctgc tcagcgagga cctcgggccc gagagccgca tcctgttccg gcgccggatc
     2041 ctggatcgcg ccgccgccat cgcgccgttt ctgacgttcg accgggaccc gtacctcgtg
     2101 attgcggacg ggcggctgtt ctggatgctc gacgcctaca ctctcacggg gcagtacccg
     2161 tactcgacgc ggaccgaagg cgggttcaac tacatccgga actccgtgaa ggtcgtcatc
     2221 gacgcccaca acggcacgac gaccttctac ctgtgggaac cggcggaccc gctcgcggtg
     2281 acgatcggcc gggtgttccc ggggctgctc cggccgatgg ccgagctgcc tgaaccgctg
     2341 cggcgccacg tgcgctaccc ggaggggatc ttcagcgtgc agagccggat gtacgcgacg
     2401 taccacatga cgaacccgtc ggtcttctac aacaaggaag accagtggga agtgccgtcg
     2461 atcgagtcgg ggcgaacgtc ggcgccgatg gagccctact acacgatcat gcgcctgccc
     2521 caggagcggg aggctgagtt cgtccagatg ctgccgttca cgccgcggcg caaggacaac
     2581 ctcgccgcgt ggatggtggc gcgcagcgac ggcagccatt acggccggat gctggtgttc
     2641 cagttcccga agcagaaggt cgtgttcggc ccccggcaga tcgtagcccg catcaatcag
     2701 gaccaggtca tcgccccgca gatcacgctg tggaaccagc aggggtccga ggtcatccag
     2761 ggcacgcttc tcgtcattcc gctggaggag tcgctgctct acgtgcggcc cttgtacctc
     2821 cgggcggcgg gcggcaagat cccggagctc aagcgggtca tcgtcgccca tcagaacctg
     2881 attgtgatgg aggagtcgct cgacgcggcg ctcgaccgca tcttccgcaa gggcggcccc
     2941 gcccggccgg ctgccgcgga cgccgcgtcg aatggcgcgc cggcgttgtc tgccgcagcg
     3001 ccgctccagg cggaggagcc gctcgaggcg ctcgccggcg ctgcgcgcca gcacttcgag
     3061 cgggccatcc aggcgcagcg cgacggcgac tggg
//
LOCUS       DAOSIF010000380         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27474_length_3092_cov_3.107014, whole genome shotgun sequence.
ACCESSION   DAOSIF010000380 DAOSIF010000000
VERSION     DAOSIF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27474_length_3092_cov_3.107014"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2747)
                     /gene="uvrA"
                     /locus_tag="PLT35_11345"
     CDS             complement(<1..2747)
                     /gene="uvrA"
                     /locus_tag="PLT35_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG29862.1"
                     /translation="MAETGAPETAGIVVRGARTHNLKNVSLTLPSGRLVVITGVSGSG
                     KSSLAFDTIYAEGQRRYVESLSAYARQFLERMEKPDVDAVDGVSPAIAIRQKNSIRNP
                     RSTVGTTTEIYDYLRLLFARVGRTFCRQCGQEVMRETAEVVASRLASLPAGTRLLLGF
                     DVAMLPVAPESAELADAVEEIAEGGLEDGDGVESPSAAGADGAPAPDGAMNGDGRSAA
                     VAATLEALRKKGFRRLLVEGRAADFDEADPAALAGRPELHVIVDRLVVEPGIVARLTD
                     SIETAYAEGDGAAFAVELPDRMHVFSERFECRACGIAYEDPQPRLFSFNNPYGACATC
                     HGFGNIIEIDLDLVVPDPSKSINQNAIEPWSKPHYRSCLATLKRAARRRGVRLDVPWT
                     SLTADERAFVVDGDGEEYEGIRGFFRWLDRKKYKVHVRVFLSRYRGYLTCPDCGGTRL
                     RPEARDVRVGGRTIDEVCRLTVKDARRFFAGLALGEKDAAVAEKLLGEIRRRLEFLSD
                     VGLDYLTLDRLSSTLSGGESQRISLASALGSALVGTLYVLDEPSIGLHPRDNGRLIDI
                     LHELRDQGNTVLVVEHDADMIRVADHIVDLGLGAGEQGGRVIYSGGLEGLLGEPRSLT
                     ARYLRGELSIAVPAARFHGSGQRLRVLGAAEHNLRGIDVDIPLNTLTVVTGVSGSGKS
                     TLVHGILYPGIKRLKGGVERRVGAHRRIEGAEYVTDAILVDQSPIGRSPRSNPVTYLK
                     AFDPIRELFAATKDARAAGLTASHFSFNVPGGRCEACEGEGQVRVEMQFLADVFVPCD
                     VCEGRRFKPRVLEVALRGKTIHQVLEMTVREALGFFSGSPKVLRRLHVLDEIGLGYLR
                     LGQPATTLSGGEAQRIKIAAHLAASGGERLLYILDEPTTGLHFDDIARLLAAFRRLLQ
                     AG"
BASE COUNT          452 a         1137 c         1057 g          446 t
ORIGIN      
        1 cccgcctgca gcagccggcg gaaggcggcg agcagcctgg cgatatcgtc gaagtgaagc
       61 ccggttgtcg gctcgtcgag aatgtagagc aggcgctcgc cgccggaggc ggccaggtgg
      121 gccgcgatct tgatccgctg cgcctcgccg cccgacagcg tcgtcgccgg ctggcccagc
      181 cgcaggtaac ccaggccgat ctcgtcgagc acgtgcaggc gccgcagcac cttcggcgag
      241 ccgctgaaga accccagcgc ctcccggacg gtcatctcga gcacctggtg gatggtcttc
      301 ccccgcagcg ccacctcgag gacgcgcggc ttgaaccgcc tcccctcgca gacgtcgcag
      361 ggcacgaaga cgtcggcgag gaactgcatc tccacccgca cctggccctc cccttcgcag
      421 gcctcgcacc ggccgccggg aacgttgaag gagaagtgac tggccgtcag cccggcggcg
      481 cgggcgtcct tcgtggccgc gaacagctcg cggatggggt cgaacgcctt caggtacgtc
      541 accgggttcg agcgcggcga ccgcccgatg ggcgactgat cgaccaggat cgcgtcggtc
      601 acgtattcgg cgccctcgat gcggcggtgc gccccgacac gccgctcgac gccgcccttc
      661 aggcgcttga tccccgggta gaggatgccg tgcacgagcg tggacttgcc cgaaccgctc
      721 acgccggtga cgaccgtgag cgtgttcagc gggatgtcca cgtcgatgcc gcgcaagttg
      781 tgctcggcgg cgcccagcac ccggaggcgc tggcccgagc cgtgaaacct cgccgcgggc
      841 accgcgatcg acagttcgcc gcgcaggtac ctggccgtga gagaccgcgg ctcgcccagg
      901 agcccctcga ggcctccgct gtagatgacc cgcccgccct gctcgcccgc cccgagcccg
      961 aggtcgacga tgtgatcggc cacgcggatc atgtcggcgt cgtgctcgac gacgagcacc
     1021 gtgttcccct ggtcgcgcag ctcgtgcagg atgtcgatca ggcggccgtt gtcgcgcggg
     1081 tggaggccga tcgacggctc gtcgaggacg taaagcgttc cgaccagcgc cgagccgagg
     1141 gccgaggcga ggctgatccg ctgcgactcg ccgcccgaca gcgtcgagga caggcggtcg
     1201 agcgtcaggt agtccaggcc cacgtcgctc aggaactcaa gccgccggcg gatctcgccc
     1261 agcagcttct ccgccaccgc cgcgtccttc tcgccgagcg ccaggcccgc gaagaagcgc
     1321 cgcgcgtcct tgacggtcag ccggcacacc tcgtcgatcg tccggccgcc cacccgcacg
     1381 tcgcgcgcct ccggccgcag gcgcgtgccg ccgcagtcgg ggcacgtcag gtagccccgg
     1441 taccggctca ggaacacgcg gacgtgcacc ttgtacttct tgcgatcgag ccagcggaag
     1501 aacccgcgga tgccctcgta ctcctcgccg tctccatcca ccacgaacgc ccgctcgtcg
     1561 gccgtcaggc tcgtccacgg gacgtccagg cgcacgcccc tccggcgcgc cgcccgcttg
     1621 agcgtcgcca ggcagctccg gtagtgcggc ttgctccacg gctcgatggc gttctggttg
     1681 atggacttgg acgggtccgg caccaccagg tccagatcga tctcgatgat gttcccgaac
     1741 ccgtggcagg tggcgcaggc gccgtacggg ttgttgaacg agaacagccg cggctgcgga
     1801 tcctcgtacg cgatcccgca ggcccggcac tcgaagcgct ccgagaacac gtgcatgcgg
     1861 tcgggcagct ccacggcaaa ggcggccccg tcgccctcgg cgtaggccgt ctcgatcgag
     1921 tcggtcagcc gggccacgat gcccggctcc acgacgaggc gatcgacgat cacgtgcagt
     1981 tcgggccggc ccgcgagcgc cgccggatcg gcctcgtcga agtccgccgc gcgcccctcg
     2041 acgagcagcc tccggaagcc cttcttccgc agcgcttcga gcgtggccgc caccgccgcg
     2101 ctcctgccgt cgccgttcat ggcgccgtcc ggggccggcg ctccgtccgc gccggctgcc
     2161 gacggactct ccaccccgtc gccgtcctcc aggcccccct ccgcgatctc ctcgacggcg
     2221 tccgccagct ccgcggactc gggcgcgacg gggagcatcg cgacatcgaa gcccaacaac
     2281 agcctcgtgc ctgccggcag agacgccagc cgcgaggcga ccacttcggc cgtctcccgc
     2341 atgacctcct gcccgcactg gcggcagaag gtccggccga ccctggcgaa aagcaggcga
     2401 aggtaatcgt agatctcggt ggtcgtgccg acggtcgagc gcgggttccg gatgctgttc
     2461 ttctggcgaa tcgcgattgc cggcgagacg ccgtcgacgg catccacgtc cggcttctcc
     2521 atccgctcca ggaactggcg ggcgtacgcc gacagcgact cgacgtagcg gcgctggccc
     2581 tcggcataga tcgtgtcgaa ggcgagcgac gacttgcccg agccgctgac gccggtgatc
     2641 accaccaggc ggccggacgg cagcgtcagg ctgacgttct tgaggttgtg ggttcgcgcc
     2701 ccgcggacga cgatgcccgc cgtttcaggg gcgccggttt ctgccatgtc gcgccgctgc
     2761 cctactgtgc cgtcgtgagg tggaccccta aatcaacaat cgtagcacac gggcgcagaa
     2821 gcagagggga tcgggggttg aggatcggag accggggatc ggagactggg gatcgcgaac
     2881 cggggatcgg gaaccgggga ttggcgaccg gggattgggg ggccggcggc cagccgccgg
     2941 cccccggccc ccggcccccc gccccccggc tctcagagga ggttggtgta cttgctccag
     3001 aggtcctccg ccagcttcca gtaggcgtcg acggcgccgt tggcgacctc gtgcgagtac
     3061 ttcgtcagga acgcgcgggc cttcgccggg tc
//
LOCUS       DAOSIF010000381         3091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27489_length_3091_cov_4.015152, whole genome shotgun sequence.
ACCESSION   DAOSIF010000381 DAOSIF010000000
VERSION     DAOSIF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27489_length_3091_cov_4.015152"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2697
                     /gene="mutS"
                     /locus_tag="PLT35_11350"
     CDS             <1..2697
                     /gene="mutS"
                     /locus_tag="PLT35_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG29863.1"
                     /translation="IVSPADRIASPQAIAAATPAMRQYLDAKRQYRDAIVFFRMGDFY
                     EMFYEDALVAARALDLTLTSRAKDADGGSIPMCGVPYHAADAYLTRLVRKGFRVAICE
                     QVEDPRKATGLVKREVIRVVSPGTFTEGGYLDAREPVFLMAISPALPVPAGGAQGRAA
                     AGLDATGVALLDLSTGEFLTAEYAGRDGLQALRDELGVLRPRELLIPSDIPAEEVVPA
                     ALRGGASVTPAEPWTFDLDGARRALLSQLRTHSLEGFGLEAHPAAIRAAGALVHYLKD
                     TQKSDLTHVRAVSFKEHADRLVVDPLTLKHLEVVESFDGGRAGSLLHELDRTITPMGA
                     RLLRTWLIRPLLSLERVRDRLDAVEDFGFRTIDRGRFREALRGMHDVERLVARVALGT
                     AGPRDLVAIRTSLSAVPRLRQILEGFQAPFVSSLAAELDEVAEVRDAIERTLVDEPPA
                     LARDGGCVRSGVDAELDDLRRVSASGKQIIAELEEQERARTGIASLKVRYNRVFGYYI
                     EVSKPNLHAVPADYHRKQTIAGGERFITPALKAHEERVLGADERILERELELFEALRS
                     AVSAEAPRMQDTARAVATLDVLAALADAAAEHNYVKPHVHEGSEIAILDGRHPVVERY
                     SPDPFVPNDTHLDGAARQVVILTGPNMGGKSTYLRQVALIALMAQAGSFVPAREAKLA
                     LVDRVFARVGASDNIARGQSTFMVEMQETANILYSATSRSLVILDEIGRGTSTFDGLS
                     IAWAVAEHLASGPRARPKTLFATHYHELTELADSLSGVVNAHVAAREWRDDIVFLRKV
                     LPGRSDRSYGIQVARLAGLPPTVIARARAILTALEQDALAKGGRPAVAASGQDPRAQL
                     GLFDAAAPGDGIVRRLKDADLDRLTPIEALAFLAELQRDIGP"
     gene            2694..>3091
                     /locus_tag="PLT35_11355"
     CDS             2694..>3091
                     /locus_tag="PLT35_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29864.1"
                     /translation="MTLSGRRLSAAVCAAAALLAAAGQASAGRYHPRLKFQVLRTPHF
                     TIYYHQGEAELARRLAAIAEDVRRDLPSRAALEPPPHVHVVLVDQSDVANGWSTPVPY
                     DLIEVAAAPAAPSSFLGHQDDWLRTVFTHE"
BASE COUNT          448 a         1096 c         1092 g          455 t
ORIGIN      
        1 atcgtgtcac ccgccgaccg gatcgcctcg ccgcaagcca tcgccgcggc cacgcccgcc
       61 atgcggcagt acctggatgc caagcggcag taccgcgacg ccatcgtctt cttccgaatg
      121 ggcgacttct acgagatgtt ctacgaagac gcgctggtgg cggcgcgcgc cctggacctg
      181 acgctgacct cgcgggcgaa ggacgccgac ggcggcagca ttccgatgtg cggcgtgccc
      241 taccatgcgg ccgacgccta cctcacccgg ctggtccgca agggattccg agtggcgatc
      301 tgcgagcagg tggaagaccc gcgcaaggcc acggggctgg tgaagcggga ggtcatccgc
      361 gtcgtgtcgc cgggcacgtt caccgagggc ggctatctgg acgcaagaga gccggtcttc
      421 ctgatggcca tctcccctgc gctccccgta cccgcaggag gcgcgcaggg acgagccgca
      481 gccggccttg acgccacggg cgtcgcgctg ctcgacctct caaccggcga gttcctgacg
      541 gcggagtacg ccgggcggga cggcctgcag gcgctgagag acgaactggg agtcctgcga
      601 ccgcgcgagc tgctgattcc gtccgacatc ccggctgagg aagtggtccc ggcggcgtta
      661 cggggaggcg ccagcgtcac cccggccgag ccgtggacct tcgacctcga cggggcacgc
      721 cgggccctcc tgtctcagct gcgcacgcac tcgctcgagg ggtttggcct cgaggcgcat
      781 ccggccgcca tcagggcggc cggggcgctg gtccactacc tcaaggacac ccagaagtcg
      841 gacctgaccc acgtgcgggc ggtgagtttc aaggagcacg ccgaccgcct cgtcgtggac
      901 ccgctgacgc tgaagcacct ggaggtggtc gaatcgttcg acggcgggcg ggcggggtcg
      961 ctgctgcacg agctcgaccg cacgatcacg cccatgggag cccggctcct gcggacctgg
     1021 ctcatccggc cgctgctctc gctcgagcgc gtccgcgacc ggctggacgc ggtggaggac
     1081 ttcggcttcc ggacgatcga ccgcggccgc ttccgggagg cgctgcgagg catgcacgat
     1141 gtcgagcggc tcgtcgcccg tgtcgcgctg ggcacggccg gcccgcgcga tctcgtggcc
     1201 atcaggacgt cgctctcggc agtcccgcgc ctccggcaga tcctggaggg gtttcaggcg
     1261 ccgttcgtct cgagcctggc cgcggagctc gacgaggtcg ctgaggtccg ggacgcgatc
     1321 gagcggacgc tcgtcgacga gccgccggcc ctggcccgtg acggcggatg cgtacggagc
     1381 ggcgtcgacg cggagctcga cgacctgcgc cgcgtatcgg cgtcgggaaa gcagatcatc
     1441 gcggagctcg aggagcagga acgcgcgagg acgggcatcg cctcgctcaa ggtccgctat
     1501 aaccgggttt tcggctacta catcgaggtg tcgaagccga acctccacgc cgtgccggcg
     1561 gactaccatc gcaagcagac gatcgcgggc ggagagcgct tcatcacgcc ggcgctgaag
     1621 gcccacgagg agcgcgtcct cggcgccgac gagcggatcc tggagcggga gctggagctg
     1681 ttcgaggccc ttcgctcggc cgtgtcggcg gaagcaccgc ggatgcagga caccgcccgc
     1741 gccgtcgcga cgctggacgt gctggcggcc ctggccgatg cggcagccga gcacaactac
     1801 gtcaagccgc acgtgcacga gggcagcgag atcgcaatcc tcgatggccg ccatccggtc
     1861 gtcgaacggt acagcccgga cccgttcgtg ccgaacgaca cgcacctgga cggcgcggcg
     1921 cggcaggtcg tcatcctcac cgggccgaac atgggcggca agtccaccta cctgcggcag
     1981 gtcgccctca tcgcgctgat ggcgcaggcg gggtcgttcg tcccggcgcg cgaagccaag
     2041 ctcgccctgg tggatcgggt gttcgcccgg gtcggcgcct cggacaacat cgcgcggggc
     2101 cagtccacct tcatggtgga gatgcaggag acggcgaaca tcctgtactc ggcgacgtcg
     2161 cggagcctcg tgattctgga cgagatcggg cgcggcacgt cgaccttcga cggcttgagc
     2221 atcgcgtggg cggtagccga gcacctggcg tccggtccgc gggcccgtcc gaagactctg
     2281 tttgcgacgc actaccacga gctgacggag ctggccgact cgctgtcggg cgtggtcaac
     2341 gcgcacgttg ccgcccggga gtggcgcgac gacatcgtgt tcctgcggaa ggtccttccg
     2401 gggcgctcgg atcggagtta cggcatccag gtggctcgcc tggccgggct gccgccgacg
     2461 gtcatcgccc gcgctcgcgc cattctcacg gccctcgagc aggatgctct ggcaaagggc
     2521 ggacgcccgg ccgtggcggc gtcggggcaa gacccgcgcg cgcagctcgg cttgttcgac
     2581 gctgcggcgc caggcgacgg gatcgtgcgt aggctgaagg acgccgatct cgatcgcctc
     2641 acgccgatcg aggcgctcgc cttcctggcc gagctgcaac gggacatcgg cccgtgaccc
     2701 tttcgggccg tcggctgtcg gcggcggtgt gcgccgcggc ggccctcctg gccgccgccg
     2761 ggcaggcgag cgcgggccgg taccaccccc gattgaagtt ccaggtcctc cgcactccgc
     2821 atttcaccat ctactatcac cagggcgaag cggagctcgc gcgccgcctc gccgcgatcg
     2881 ccgaggacgt tcgccgcgac ctcccctcgc gcgcggccct ggagccgcct ccccacgtcc
     2941 acgtcgtcct ggtcgaccag tccgacgttg ccaacggctg gtccacgccc gtgccctacg
     3001 acctcatcga agtcgcggcg gcgcccgccg cgccgtcttc gttcctcgga catcaggacg
     3061 actggctgcg gaccgtcttc acgcacgaat t
//
LOCUS       DAOSIF010000382         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27739_length_3067_cov_5.026228, whole genome shotgun sequence.
ACCESSION   DAOSIF010000382 DAOSIF010000000
VERSION     DAOSIF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27739_length_3067_cov_5.026228"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLT35_11360"
     CDS             complement(<1..740)
                     /locus_tag="PLT35_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29865.1"
                     /translation="MEPRKPFTEAAGGSPTRREFLHQAAAAAGTVGALTILPGRVFGG
                     QRKLAPSDKLNLGAVGVGGMGGSNLSRCETENIIALCDVDSALAAKTFGKYPNARRYG
                     NYREMFDKEKDLDAVIVATPDHTHAVVAMAAMQRGLHVYVQKPIAHAVWEARLMTETA
                     RKHKISSQMGNQGHSGEGLRLISEWVAAGALGQIKEVHAWTNRPVWPQGVEVERPKDT
                     PPVPATMDWDEWIGPAPSRPFHPTYHPGR"
     gene            1011..2336
                     /locus_tag="PLT35_11365"
     CDS             1011..2336
                     /locus_tag="PLT35_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG29866.1"
                     /translation="MKDITRRDFVKRAAAATVLLGTSRTAWAGANDRLRIAVLGLNGR
                     GKSHIGDYDRLERVEVVTLCDPDARLFAPTVKAFFTDKAKAAPKVEQDIRRVLEDKDV
                     DAISIATPNHWHSLATIWACQAGKDVYVEKPMTHNVFEGKRVVEAVKKYGRIVQHGTQ
                     LRSNPGFQEGIRLLHEGAIGRVYLARCVCYKWRPDIGKGRPGQPPKELDWNVWQGPAQ
                     EEPFIVDAKGDGVFVHYNWHWHWAYGNGDIGNQGIHQVDAARWGLGVGLPYRVTSMGG
                     RLLWDDAKQIFDVSSTSFMFKGKDGQDKMMTLEVRPWMTNDEAGGREFGVMFFGEKGW
                     MSFPGYGGYKMYEGRQNTLVKEREDGSDLNHYRNFVECVRSRDMAALNAPAAEGHWSS
                     ALSHYALTGARVNRVLEIDPATELVKGDEEANAHLTRKYRAPFTVPDAV"
     gene            2355..>3067
                     /locus_tag="PLT35_11370"
     CDS             2355..>3067
                     /locus_tag="PLT35_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOG29867.1"
                     /translation="MPRRAVVVPLAAGCLLLAAGAAAAQPAPRPNVIVILADDLGYGD
                     VGAYGQRIIQTPNLDRMASEGIRFTQFYAGSTVCAPSRSVLMTGQHLGHTRVRGNAGA
                     GNDEAQTLAPGEATVARVLQQAGYATALVGKWGLGEIGSAGEPTRQGFSEFYGVVNQT
                     HAHNHYPDFLWRNTQRIALPNVVTPVGQVPGAGYATRRVQYANDLFFDEARAFLDRSR
                     DRPFFLLLSLTVPHANNERA"
BASE COUNT          460 a         1063 c         1110 g          434 t
ORIGIN      
        1 cggccggggt gataggtggg gtggaacggc cggctcggcg ccgggccgat ccactcgtcc
       61 cagtccatcg tggccggcac gggcggcgtg tccttcggcc gctccacctc gacgccctgc
      121 ggccacacag gccggttcgt ccaggcgtgg acttccttga tctggccgag cgccccggcg
      181 gcgacccact ccgagatgag ccggagcccc tcgcccgaat ggccctggtt gcccatctgc
      241 gacgagatct tgtgcttgcg ggccgtctcg gtcatcagcc gcgcctccca cacggcgtgg
      301 gcgatgggct tctgcacgta gacgtggagg ccgcgctgca tcgccgccat cgcgacgacg
      361 gcgtgggtgt gatcgggggt ggcgacgatg accgcgtcga ggtccttctc cttgtcgaac
      421 atctcgcggt agttgccgta ccgccgcgcg ttcgggtact tcccgaaggt cttggccgcg
      481 agcgccgagt ccacgtcgca cagcgcgatg atgttctccg tctcgcagcg gctgaggttc
      541 gatccgccca tgcccccgac gccgacggcg ccgaggttga gcttgtcgct cggcgcgagc
      601 ttgcgctgcc cgccgaacac gcggccgggc aggatcgtga gcgcaccgac ggtcccggcg
      661 gctgccgcgg cctggtgcag gaactcgcgg cgggtgggcg acccgccggc ggcttcagtg
      721 aacggcttcc tgggttccat gagcactctc ctcgctgcgg ggcgccagcg ccggctcgcg
      781 agcctggccc ggccgcgggc gcggccggga ggtttcccgg cctccgtcgg ccggcctgac
      841 ggcgggtagt atcgcaggtc gccggcgcgg cttcaactcg cgcggagcac ggccccctcc
      901 gaagggccgg accggggggc ggcgcggccg cgatcccctc cgccgccggg cgggtagaat
      961 aggcctcgcc ggcagcgggc ttcgtcggat ccgcagggag gacgatcagc atgaaggaca
     1021 tcacacgcag ggacttcgtc aagcgcgccg ccgcggcaac cgtgctgctg ggcacgtcca
     1081 ggaccgcctg ggccggcgcg aacgaccgcc tccggatcgc cgtgctgggg ctcaacgggc
     1141 gcggcaagtc ccacatcggc gactacgacc ggctcgagcg cgtcgaggtc gtcacgctgt
     1201 gcgatcccga tgcgcggctg ttcgcgccaa ccgtcaaggc gttcttcacc gacaaggcca
     1261 aggccgcccc gaaggtggag caggacatcc gccgcgtcct cgaggacaag gacgtggacg
     1321 cgatctccat cgccacgccg aaccactggc actcgctggc cacgatctgg gcctgccagg
     1381 ccggcaagga cgtgtacgtc gagaagccga tgacgcacaa cgtcttcgag ggcaagcgcg
     1441 tcgtcgaggc ggtgaagaag tacggccgga tcgtgcagca cggcacgcag ctgcgcagca
     1501 acccggggtt ccaggagggc atcaggctgc tgcacgaggg cgccatcggc agggtctacc
     1561 tggcccggtg cgtctgctac aagtggcgcc cggacatcgg gaagggccgg cccggccagc
     1621 cgcccaagga gctggactgg aacgtgtggc aggggccggc ccaggaagag cccttcatcg
     1681 tcgacgccaa gggcgacggc gtgttcgtgc actacaactg gcactggcac tgggcctacg
     1741 gcaacggcga catcggcaac cagggcatcc accaggtcga tgccgcgcgc tgggggctcg
     1801 gcgtgggcct gccgtaccgg gtgacgtcga tgggcggcag gctgctctgg gacgacgcga
     1861 agcagatctt cgacgtgtcg tcgacgtctt tcatgttcaa gggcaaggac ggccaggaca
     1921 agatgatgac gctcgaagtg cgcccctgga tgacgaacga cgaggccggc ggccgggagt
     1981 tcggggtgat gttcttcggc gagaaggggt ggatgagctt cccgggctac ggcggctaca
     2041 agatgtacga gggccgtcag aacacgctgg tgaaggagcg ggaggacggg tcggacctga
     2101 accattaccg caacttcgtc gagtgcgtcc gcagccgcga catggccgcc ctcaacgcgc
     2161 cggccgccga ggggcactgg tcctcggccc tctcgcacta cgcgctcacg ggcgcacggg
     2221 tcaaccgcgt gctggagatc gatccggcga cggagctcgt gaagggcgac gaggaagcca
     2281 acgcgcactt gacgcggaag taccgggcgc cgttcacggt gcccgacgcg gtctgacggc
     2341 ggagcggacg gaccatgccg cgccgcgccg tcgtcgtgcc gctggcggcc ggctgcctgc
     2401 tcctggcggc cggcgctgcc gccgcccagc ccgcgccgcg cccgaacgtc atcgtcatcc
     2461 tggccgacga tctcggctac ggcgacgttg gcgcgtacgg gcagcggatc atccagacgc
     2521 cgaacctcga ccggatggcg tccgagggga tccgcttcac gcagttctac gcgggctcca
     2581 ccgtctgcgc gccgtcgcgc agcgtgctca tgaccgggca gcacctgggg cacaccaggg
     2641 tgcgcgggaa cgcgggcgcg gggaacgacg aggcgcagac gctggccccg ggcgaggcga
     2701 ccgtcgcccg cgtgctgcag caggccggct acgccacggc cctcgtcggc aagtgggggc
     2761 tcggcgagat cggatcggcg ggggagccga cccgtcaggg tttctccgag ttctacggcg
     2821 tcgtcaacca gacgcacgcg cacaaccact atcccgactt cctctggcgg aacacgcagc
     2881 ggatcgcgct tcccaacgtc gtgacgccgg tcggccaggt gccgggcgcg ggctacgcga
     2941 cccgccgcgt gcagtacgcc aacgacctct tcttcgacga ggcgcgcgcc ttcctcgatc
     3001 ggagccggga ccggccgttc ttcctgctcc tctcgctgac cgttccgcac gcgaacaacg
     3061 agcgggc
//
LOCUS       DAOSIF010000383         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27783_length_3064_cov_4.428714, whole genome shotgun sequence.
ACCESSION   DAOSIF010000383 DAOSIF010000000
VERSION     DAOSIF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27783_length_3064_cov_4.428714"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="PLT35_11375"
     CDS             <1..1654
                     /locus_tag="PLT35_11375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG29868.1"
                     /translation="ADPPWQMSVGVVWHDEGAWTHNARNMALFGTWETDRWNPMYLAP
                     VFTALEYASFRAFGVGLWQARLVPEILGAASVLFLAGAVRHAAGRAAGALAGALASVH
                     FVWVMWNRAALLETPMVVPMVAALYACARGARAGGWRAAAWGASAGACAVIAFFAKAA
                     AAFFLPALGVVALAGLWRWHVAPADARAAERSDLAHRGVILGLLLAGATALVVFVIPF
                     WSEFRFYNWQMSVTRKPSYAWAAFVDRASWLPVVTDFFARMWVVLLLAFGAACGVAAR
                     WRRAAAAEWLAVAWIVLGCAELIVHDTGNERRYVFLIPPLIALASIALGRDRRLVAPE
                     VAALTRRQLVAALPVVLFGFYVLAGSAARAAFAPRISPSVRAGAAAAALAGAALAVAW
                     PRPVRWISSRRWTLRASVTLAALVVGLDLAGFVRWSSGRTYHNYRAMRLVGERLAPGT
                     LVHGKLANGLALESRIRPVFVGREFGNYEDRLERDDIRYILTYTRPYLGYEGRVILDV
                     LGATAWTVVERFPVAESPAGNDEAALIEKLPRAGSGTPAERH"
     gene            1655..>3064
                     /locus_tag="PLT35_11380"
     CDS             1655..>3064
                     /locus_tag="PLT35_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29869.1"
                     /translation="MSDTHAVGGPLEQARPGARVRRSAAPARMAAFVFLVVAASYLSA
                     AAAGFGFVSDDFMILQRLARTPGVQGALAFFSQPYYDYYRPLGFLSFAVDWSFWRDWP
                     AAYHLTSLALHAANMWLVWALATRLAGPAAGLVAAAIFGLHPANHEAVYWMSARFDLL
                     AACLALGSLLLLQGRSLRCAACALLYLAALLSKESAVAFPVVVFAYAWLIRRESLAVL
                     LRVLGWMGGAALVYTFLRQASGLPPAGGASRLPKLLAFAVLLGLQLALAHPGSAGVRR
                     WMWDRRRPLAAAAASALGALGILAAAWPPAAALRGAFASLGFAAIHLVSPVSPDRWLT
                     PLPWWIGLAGGAAAVGMIGAASRMASGAVPAFLAWFLAAALLPVSSMTEGPRYLYLAS
                     VPVAIFAARILITVLRRDSVPVRVLGAVLIAALGGQIAARGRDWVWASGMTAEAAAAI
                     AQAAGPGCRGARIVLATAPV"
BASE COUNT          283 a         1125 c         1122 g          534 t
ORIGIN      
        1 ggccgacccg ccctggcaga tgagcgtcgg cgtggtgtgg cacgacgagg gagcgtggac
       61 ccacaacgcc cgcaacatgg cgctcttcgg cacctgggag accgaccggt ggaatcccat
      121 gtacctggct cccgtcttca ccgcactcga gtacgcctcg ttcagggcct tcggcgtggg
      181 gctgtggcag gcgcggctcg tgcccgagat cctcggcgcg gcgtccgtcc tgttcctcgc
      241 cggcgcggtg cggcacgccg cggggcgtgc ggcgggcgcg ttggctggcg cgctggcctc
      301 ggtgcacttc gtgtgggtga tgtggaaccg cgcggcgctc ctcgagacgc cgatggtggt
      361 gccgatggtg gccgcgctgt acgcctgcgc ccggggcgcg cgcgccggcg ggtggcgcgc
      421 ggccgcgtgg ggcgcctccg ccggcgcctg cgccgtgatc gcgttcttcg cgaaggcggc
      481 ggccgcgttc ttcctgccgg ccctcggcgt cgttgcgctc gccggcctct ggcgatggca
      541 cgtcgcgccg gcggacgcgc gcgccgccga acgctcggac ctggcccacc gcggcgtcat
      601 cctcggcctc ctgctggccg gcgcgacggc gctcgtcgtt ttcgtcatcc cgttctggag
      661 cgagttccgg ttctacaatt ggcagatgtc ggtcacgcgg aagccgagct acgcctgggc
      721 tgcgttcgtc gaccgcgcga gctggctgcc agtggtgacc gactttttcg cgcgcatgtg
      781 ggtcgtgctc ctgctcgcgt tcggcgcggc ctgcggcgtg gccgcccgtt ggaggcgagc
      841 ggcggcggcc gagtggctcg cggtggcgtg gatcgtgctg ggatgcgccg agctcatcgt
      901 gcacgacacc ggcaacgagc gccggtacgt gttcctcatt ccgccgctca tcgcgctggc
      961 ctcgatcgcg ctcggacgcg atcgccgcct ggtcgcgccc gaggtcgccg ccttgacccg
     1021 ccggcagctc gtggccgccc tgcccgtcgt cctcttcggc ttctacgtgc tggccgggtc
     1081 cgccgcccgc gcggcgttcg ccccgcgcat cagcccaagc gtccgcgccg gcgcggccgc
     1141 ggcggcactg gccggcgccg ccctggcggt ggcctggccg cgccccgttc gctggatctc
     1201 gagccgccga tggacgctgc gggcgtcggt gaccctcgcc gccctcgtcg tcgggctcga
     1261 cctggccggc ttcgtccggt ggtcctccgg ccgaacctac cacaactacc gggccatgcg
     1321 cctcgtcggc gagcggctgg cgccagggac gctcgtgcac ggcaagctcg ccaacggcct
     1381 cgccctcgag agccgcatcc ggccggtgtt cgtcgggcgg gagttcggca actacgagga
     1441 tcgccttgag cgcgacgaca tccgctacat cctgacctac acgcggccct acctgggcta
     1501 cgagggccgc gtcatcctcg acgtgctcgg cgccaccgcc tggaccgtcg tcgaacggtt
     1561 ccctgtggcg gaatcgccgg cgggaaatga cgaggccgcc ctcatagaga agctgccgcg
     1621 ggccgggtcg ggaaccccgg cggagcgcca ctaggtgtcc gacacgcacg ccgtcggcgg
     1681 gccgctggag caggcgaggc ccggtgcgcg ggtccgccgg tccgccgcgc cggcgcggat
     1741 ggcggccttc gtgttcctgg ttgtcgcagc ctcttacctg tcggccgcag ccgcgggatt
     1801 cgggttcgtg tccgacgact tcatgatcct gcagaggctc gctcggacgc cgggcgtgca
     1861 gggagcgctg gcgttctttt cgcagccgta ctacgactac taccgccctc tcggcttcct
     1921 ctcgttcgcc gtggactggt cgttctggcg ggactggccg gccgcctacc acctgacgtc
     1981 tctggcgctg cacgcagcca acatgtggct cgtctgggcc ctcgcgacgc gcctggcggg
     2041 tccggccgcc ggcctcgtgg cggccgcgat cttcggcctt catccggcca accacgaagc
     2101 cgtctactgg atgtcggccc gcttcgacct gctggcggcg tgcctggcgc tcggctcgct
     2161 gctgctgctg cagggccgct cgctccgctg cgctgcgtgc gccctcctct atctggccgc
     2221 cctcctgtcg aaggagtcgg ccgtcgcctt tccggtcgtc gtcttcgctt acgcatggct
     2281 catcaggcga gagtctctcg cggtcctgct gcgcgtgctc ggctggatgg gcggcgcggc
     2341 gctggtctac acgttcctcc gtcaagcctc cgggcttccc cccgctggag gcgcgtcgag
     2401 gctcccgaag ctcctggcct tcgccgtcct gctgggcctg cagctggcgc ttgcccaccc
     2461 cgggtccgcc ggcgtgcgcc ggtggatgtg ggaccggcgt cggccactcg ccgccgcggc
     2521 tgcttcagcg ctcggggcgc tcgggattct ggctgccgcg tggccgccgg cggcggcctt
     2581 gcgcggggcc ttcgcttcgc tgggattcgc cgcgatccac ctcgtgtcgc ccgtgtcgcc
     2641 agatcgctgg ctcacgccgc tgccgtggtg gatcggactc gctggaggcg cggccgcggt
     2701 cggcatgatt ggagcggcca gccggatggc ctccggggcg gtccccgcgt ttctcgcgtg
     2761 gttcctggct gcggcgctgc tgcccgtgtc gagcatgacg gaaggccccc gctacctgta
     2821 cctggcgtcg gtgccggtgg cgatcttcgc ggcgcggatc ctcatcaccg tcctgcgccg
     2881 cgactcggtt cccgtccgcg tgctgggggc cgtgctgatt gcggccctag gcgggcagat
     2941 cgcggccagg gggcgcgact gggtctgggc gtcgggcatg acggcagaag cagcggccgc
     3001 catcgctcag gcggccgggc cgggatgccg gggggctcgg atcgtgctgg ccacggcccc
     3061 ggtg
//
LOCUS       DAOSIF010000384         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27791_length_3063_cov_4.353059, whole genome shotgun sequence.
ACCESSION   DAOSIF010000384 DAOSIF010000000
VERSION     DAOSIF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27791_length_3063_cov_4.353059"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLT35_11385"
     CDS             <1..208
                     /locus_tag="PLT35_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29870.1"
                     /translation="AALLAVAAARLAAGPAIAPVAGVLLRGACAVAVFGATLAASGFF
                     EPEELAVIRRAAGRAAGAVRARVP"
     gene            356..1909
                     /locus_tag="PLT35_11390"
     CDS             356..1909
                     /locus_tag="PLT35_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG29871.1"
                     /translation="MLGRRVLLFNPPLVNGVAFTRQGRCQEREEVLGTTKPPYSLALI
                     AALLRDRGFGVRLVDQTAERLSTEDLIARLNREGFAPTLLVFCSATPTASADAFEAGK
                     LKQAFGAPLVSFGPHASAAPQAAMAHAPQVDAMAVGEPEDAILQLAALESFDQAERVD
                     SLTIRRGGTVIAHRAQGAYGGFAAMPCPAWDLLPLDRYRLPLENARYLIVESSRGCPY
                     SCDFCVAPIIQGHKFRERDAVALVDEIEQGKRRFGINHFYLWGDTVTLNAKSFSRFCD
                     ELIARNLGVRWFGNGRADNLTNPEFVAKLRQSGCWMLSLGIESESDDVRREMMKKLER
                     EKIRLAIRNLREAGIKSFAFFIFGYPGDTPESIERTARFAREIDPDFANFYPAVPYPG
                     TELYEKCKREGMLVTEDWSRLEYSFYVLRSNRLDEPVVLRAIRRATRRFYLRPSWMAR
                     HVVDLLKIVRSSGPLVWQAAVRVVRGAPDAAPAAPAVAAHPPAESSAPVHRVEIVRPA
                     PRADRPPRD"
     gene            complement(1869..2639)
                     /locus_tag="PLT35_11395"
     CDS             complement(1869..2639)
                     /locus_tag="PLT35_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOG29872.1"
                     /translation="MPDGPLVSVVVPTYNERERLGRFVELLAGVLGEARLDGEIVIVD
                     DNSPDGTGALADTLAARHPVRVIHRSGKLGLGTAVMAGFAAARGEILGVMDADISHPP
                     ERLPVLVAALRQSGADVVIGSRYIPGGGTRNWPASRAVMSRAACLAARALTPVRDAAS
                     GFFVLRRPVIAGVEIKAAGFKICLELLVRGQVGSVLEVPYLFSDRAAGQSKMNHREAL
                     GYLVQLAQLFMWRLSRGAPRPAWRRLSPEEADRLAARA"
     gene            2702..>3063
                     /locus_tag="PLT35_11400"
     CDS             2702..>3063
                     /locus_tag="PLT35_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29873.1"
                     /translation="MSRRAAWIVLTLLVIIRLPSLVQPSGADQDLYAYVGQEIARGGL
                     PYLDAWDQKPPAVHYTYAVMYGVWPRPAVVAATDLLAALAVALLLLALERRLTARAGW
                     AAAAIFLLLGNPAYSRLGG"
BASE COUNT          424 a         1134 c         1060 g          445 t
ORIGIN      
        1 cgcggcgctc ctggctgtcg cggcggcgcg cctggccgcc gggcccgcga tcgcgcccgt
       61 cgccggcgtc ctgctgcgcg gcgcatgcgc cgtggccgtc ttcggcgcga cgctggctgc
      121 ctcgggcttc ttcgaacccg aggagctggc cgtcatccgc agggcagccg ggcgggcggc
      181 aggagccgtg cgcgcgcgcg tgccgtaggc tcctccgccc cggcccgctc gcccaggccc
      241 tcgccgtcgc ggcgccgcgc gtggcggact cccccgccag cagctagaat ggctggagtg
      301 ggtcgggtgc acgtcccgcc gccgaggggc gggcgtccga gaaacggaag tgccgatgct
      361 gggtcgccgg gttctgctgt tcaatccccc gctcgtcaac ggcgtggcgt tcacgcgcca
      421 gggccgctgc caggagcggg aggaagtcct cggcacgacg aagccgccct attcgctcgc
      481 cctgatcgcc gccctgctcc gcgaccgcgg gttcggcgtc cgcctcgtcg atcagacggc
      541 agaacggctc tcgaccgaag acctcatcgc ccggctgaac cgcgaggggt tcgcgcccac
      601 gctgctggtc ttctgcagcg ccacgccgac ggcctcggcg gacgccttcg aagccggcaa
      661 gctgaaacag gccttcggcg cgcccctggt gtcgttcggc ccgcacgcct ctgccgcgcc
      721 ccaggcggcc atggcgcacg cgccgcaggt ggacgccatg gcggtcggcg agcccgagga
      781 cgcgatcctg cagcttgcgg ccctcgagtc cttcgaccag gccgagcgcg tggacagcct
      841 caccatccgc cgcggcggca cggtcatcgc gcaccgggcc cagggcgcct acggcggctt
      901 cgcggccatg ccctgcccgg cctgggatct gctgcccctc gaccgctacc ggctgcctct
      961 cgaaaacgcc cggtacctga ttgtcgagtc gagccgcggc tgtccgtaca gctgcgactt
     1021 ctgcgtcgcg ccgatcattc aggggcacaa gttccgcgaa cgggacgccg tcgcgctcgt
     1081 cgacgaaatc gagcagggca agcggcgctt cggcatcaac cacttctacc tgtggggcga
     1141 caccgtcacc ctgaacgcca agagcttctc gcgcttctgc gacgaactca tcgcgcgcaa
     1201 cctcggcgtg cgatggttcg ggaacggccg cgccgacaac ctgacgaacc cggagttcgt
     1261 ggcgaagctc cggcaatcgg gctgctggat gctgtcgctg ggcatcgagt cggaatccga
     1321 cgacgtgcgc cgggagatga tgaagaagct cgagcgcgag aagatccggc tcgcgatccg
     1381 gaacctccgc gaggcgggca tcaagtcgtt tgccttcttc attttcgggt atccgggcga
     1441 cacgcccgaa tccatcgagc gcacggccag gttcgcgcgg gagatcgacc cggacttcgc
     1501 gaacttctac ccggcggtgc cctaccccgg caccgagctg tacgagaagt gcaagcggga
     1561 ggggatgctc gtcaccgagg actggtcgag gctcgagtac tcgttctacg tcctgcggtc
     1621 gaaccgactc gacgagcccg tcgtgctgcg cgccatccgg cgcgcgacgc gccggttcta
     1681 cctgcggccg tcgtggatgg cgcgccacgt cgtcgatctg ctgaagatcg tccgcagcag
     1741 cggcccgctc gtgtggcagg cggccgtgcg cgtggtgcgc ggggcgccgg acgcggcgcc
     1801 ggcggcgcct gccgttgcgg cgcatccgcc ggccgaatca tcggcgccgg ttcaccgcgt
     1861 cgaaatcgtc aggcccgcgc cgcgagccga tcggcctcct cgggactgag acggcgccac
     1921 gccggcctgg gcgccccccg ggacaggcgc cacatgaaga gctgcgccag ctgcaccagg
     1981 tagccgagcg cttcccggtg gttcatcttg ctctgcccgg ccgctcgatc gctgaagagg
     2041 tagggcacct ccagcaccga gcccacctgg cccctcacga gcagctcgag gcagatcttg
     2101 aagccggccg ccttgatctc aacgcccgcg atgacgggcc ggcgcaggac gaagaacccg
     2161 gacgccgcgt cgcgcaccgg ggtcagcgcg cgcgccgcca ggcaggcggc gcgcgacatg
     2221 acagcgcggg aagcgggcca gttcctcgtg ccgccgccgg gaatatagcg gctgccaatg
     2281 accacgtccg cgccggactg ccggagggcc gccacgagca cgggcagccg ctccggcgga
     2341 tggctgatgt ccgcgtccat cacgcccagg atctcgcccc gcgcggcggc aaacccggcc
     2401 atgaccgcgg tgccgaggcc cagcttgccg gaccggtgga tcacgcgcac ggggtgccgg
     2461 gccgccagag tgtcggccag cgcgccggtg ccgtcgggcg agttgtcgtc gacgatcacg
     2521 atctcgccgt cgagacgcgc ctcgccgagg acgcccgcga gcaactccac gaaacgcccg
     2581 aggcgctcgc gctcgttgta ggtgggcacg acgacggaca cgagcgggcc atccggcacg
     2641 aggcttctcc ttcgactatg atatctcccg ttcccgggag acgcccgggg agttcatcct
     2701 gatgagccgg cgcgccgcct ggattgtcct gaccctgctg gtcatcatcc gcctgccgtc
     2761 cctcgtccag ccgtccggag cggaccagga cctgtacgct tacgtcggcc aggaaatcgc
     2821 ccgcggcgga ctgccgtatc tcgacgcctg ggaccagaag ccgccggcgg tgcattacac
     2881 ctacgccgtg atgtacggcg tctggccccg ccccgccgtc gtcgcggcaa cggatctcct
     2941 tgccgccctc gccgtcgcgc tgctgctcct ggcgctcgag cggcgcctga cggcgcgtgc
     3001 cggctgggcc gccgcggcga tcttcctgct gctcggaaac cccgcgtatt cgagactggg
     3061 cgg
//
LOCUS       DAOSIF010000385         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27888_length_3056_cov_4.091636, whole genome shotgun sequence.
ACCESSION   DAOSIF010000385 DAOSIF010000000
VERSION     DAOSIF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27888_length_3056_cov_4.091636"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLT35_11405"
     CDS             <1..677
                     /locus_tag="PLT35_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOG29874.1"
                     /translation="ALAEPMGLEIVAPCAACYLVLSKAQHTISGYPEIAARVHKGLQA
                     AGLAYAGRAVIRHPLDVLVNAVGLDAIAAKVIRPLTGCRVAPYYGCQIVRPYSGFDDP
                     LRPQTMDRLLEALGARVVEYPLKAHCCGGSLMGTMEDIGVRMSFLLLKEARARTANMV
                     ATACPLCQYNLEAYQGQMRRTYGEDVTIPIVYFTQLMGLAFGLAPKALGIQRNLVTAD
                     PLPVTV"
     gene            687..1577
                     /locus_tag="PLT35_11410"
     CDS             687..1577
                     /locus_tag="PLT35_11410"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG29875.1"
                     /translation="MPANDTCRIGVYICHCGLNIASKVDVDALEQYAATLPKVALAKT
                     YKFMCSDPGQQLIRDDLAKHNLTHLVVASCSPLMHEPTFRGVMQEAGLNPYLYQMVNI
                     REQVSWVTTDLARATEKARLLLTAAVRRVALHDALQRSTVPVNPNVLVVGAGIAGISA
                     ALTLASAGKQVYLVEREPSIGGHMAHFDKTFPTLDCAACILTPKMTQVGKHKNIRLMA
                     YSEVEEVSGFVGNFTVRVRHKASYVDGDKCTGCGTCWEHCPGRRIPSRRVIRKDGRII
                     GQTRPGPAAPAPRPAEAGRA"
     gene            1574..1981
                     /locus_tag="PLT35_11415"
     CDS             1574..1981
                     /locus_tag="PLT35_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29876.1"
                     /translation="MSGAAAASAAAAAAEVTLGDVRAALEAATIWCPDPAVRVAGVVA
                     ADLMSDVLVDARPGFVLVTGLTTVQAIRTAAIADLAAVVFVRGKAVPPEVAALAREMN
                     VPAFASPRSLFDAAGALHAAIGHPAAAGRGLEG"
     gene            1978..2406
                     /locus_tag="PLT35_11420"
     CDS             1978..2406
                     /locus_tag="PLT35_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG29877.1"
                     /translation="MTESAFVHAYEVAGADFVNAGRASTAIKETLKHMGLAPALIRRV
                     AIAAYEAEMNAVMYARRATMTLRVTPAAVTIDVRDEGPGIPDLALAMQEGYSTATAEM
                     REMGFGAGMGLPNIRRNTDALDIASQVGRGTTVMMTVRVS"
     gene            2411..>3056
                     /locus_tag="PLT35_11425"
     CDS             2411..>3056
                     /locus_tag="PLT35_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOG29878.1"
                     /translation="MADGHYHSIRFDASACHGCMACMRVCPTQAIRVRRGVAAMIEDR
                     CIDCGECIKACPKSAVVPLTASPDGLGRFDCTIAVPSPALYTQFDGEVTPGVILQALK
                     ASGFDAVATLSWAGEAVTRALELFLADYGGPVPLISSFCPSVVRLVQIKYPELVAQLL
                     PILSPREVAAREAKRAAAEQTGLPAERIHAVYVTPCPAKMVSIVDHPGMETSYID"
BASE COUNT          456 a         1081 c         1094 g          425 t
ORIGIN      
        1 tcgcgctggc cgagccgatg gggctcgaga tcgtggcgcc gtgcgcggcc tgctacctcg
       61 tgctgtcgaa ggcccagcac accatcagcg gctacccgga gatcgcggcg agagtgcaca
      121 aagggctcca ggcggccggg ctggcctacg cgggccgcgc cgtgatccgg cacccgctgg
      181 acgtgctggt gaatgcggtg gggctcgacg cgatcgcggc gaaggtcatc cgcccgctca
      241 ccggctgccg ggtggcgccc tactacggct gtcagatcgt ccgcccgtac agcggcttcg
      301 acgatccgct ccggccgcag acgatggacc ggctgctgga ggcgctcggc gcgcgggtcg
      361 tcgagtaccc gctgaaggcc cactgctgcg gcgggtcgct gatgggcacg atggaggaca
      421 tcggcgtccg gatgagcttc ctgctcctga aggaagccag ggcgcggacg gcgaacatgg
      481 tggccacggc gtgcccgctc tgccagtaca acctggaggc ttaccagggg cagatgcgcc
      541 gcacctacgg cgaggacgtg acgatcccca tcgtctactt cacgcagttg atgggcctgg
      601 cgttcggcct ggccccgaag gccctgggga tccagcgcaa cctcgtgact gccgatccgc
      661 tgccggtcac ggtctagggg gacgacatgc ctgcaaacga cacctgccgc atcggcgtct
      721 acatctgcca ctgcggcttg aacatcgcca gcaaggtgga cgtggacgcg ctggagcagt
      781 acgcggccac gctgccgaag gtcgccctgg cgaagacgta caagttcatg tgctccgacc
      841 cgggccagca gctcatccgc gacgacctgg cgaagcacaa cctcacgcac ctcgtcgtcg
      901 cgtcgtgctc gcccctgatg cacgagccca ccttcagggg cgtgatgcag gaagcgggcc
      961 tgaacccgta cctctaccag atggtcaaca tccgcgagca ggtgtcgtgg gtgacgacgg
     1021 acctggcgcg ggcgacggag aaagcccgcc tgctcctcac cgcggcggtg cggcgcgtcg
     1081 cgctgcacga cgcgctgcag cgctcgaccg tgccggtcaa cccgaacgtg ctcgtcgtgg
     1141 gcgccggcat cgcgggcatc tccgccgcgc tgaccctggc cagcgccggg aagcaggtct
     1201 acctcgtcga gcgcgagccc agcatcggcg ggcacatggc gcacttcgac aagactttcc
     1261 ccaccctcga ctgcgcggcg tgcattctga cgccgaagat gacgcaggtg ggcaagcaca
     1321 agaacatccg cctgatggcg tacagcgagg tggaggaggt cagcgggttc gtcgggaact
     1381 tcacggtcag ggtgaggcac aaggcgagct acgtggacgg cgacaagtgc accggctgcg
     1441 gcacgtgctg ggagcactgc cccgggcggc ggattccctc gcgccgcgtc atccggaaag
     1501 acgggcggat catcggccag accaggcccg ggccggccgc gccggcgccg cgcccggcgg
     1561 aggcgggccg cgcatgagcg gcgcggcggc ggcgagcgcg gcggcggccg ccgccgaggt
     1621 cacgctgggc gacgtgcgcg cggcgctgga ggcggcgacg atctggtgcc ccgacccggc
     1681 cgtgcgcgtc gcgggcgtgg tggcggcgga cctgatgagc gacgtcctcg tcgacgcgcg
     1741 gccgggcttc gtcctggtca ccggcctcac gaccgtgcag gcgatccgca ccgccgccat
     1801 cgccgacctc gccgccgtcg tcttcgtccg cggcaaggcg gtgccgcccg aggtggccgc
     1861 cctcgcgcgc gagatgaacg tgccggcctt cgccagcccc cgctcgctct tcgacgcggc
     1921 cggagcgctc cacgccgcga tcgggcaccc ggcggcggcc ggccgaggat tggagggatg
     1981 accgagtcgg cgttcgtcca cgcgtacgag gtcgccgggg ccgatttcgt gaacgccggg
     2041 cgcgcgtcga cggcgatcaa ggagacgctg aagcacatgg ggcttgcgcc cgcgctcatc
     2101 cgccgcgtcg ccatcgcggc ctacgaggcc gagatgaacg ccgtcatgta cgcgaggcgc
     2161 gccaccatga cgctccgcgt cacgcccgcc gccgtcacga tcgacgtgcg cgacgaaggg
     2221 ccgggcatcc ccgacctcgc cctcgccatg caggaggggt actcgacggc cacggcggag
     2281 atgagggaaa tggggttcgg cgcgggcatg ggcctgccga acatcaggcg gaacacggac
     2341 gccctcgaca tcgcgtcgca ggtggggcgc gggacgacgg tgatgatgac ggtgcgcgtg
     2401 agctgaggac atggcggacg ggcactacca ctcgatccgc ttcgacgcgt cggcctgcca
     2461 cggctgcatg gcctgcatgc gggtctgtcc gacgcaggcc atccgcgtca ggcgcggcgt
     2521 cgccgccatg atcgaggacc gctgcatcga ctgcggcgag tgcatcaagg cctgcccgaa
     2581 gagcgcggtc gtgccgctga cggcgtcgcc cgacggcctc ggcaggttcg actgcacgat
     2641 tgcggtgccc tcgcccgcgc tctacaccca gttcgacggc gaggtcacgc cgggcgtcat
     2701 cctgcaagcc ctgaaggcca gcgggttcga cgccgtggcc acgctctcgt gggccggcga
     2761 ggccgtcacc cgggcgctcg agctctttct ggccgactac ggcgggcccg tccccctcat
     2821 ttcgtccttc tgcccgagcg tggtccggct ggtgcagatc aagtaccccg agctggtcgc
     2881 tcagctgctg ccgatcctct ccccgcgcga ggtcgcggcg cgcgaggcca agcgcgcggc
     2941 cgccgagcaa acgggccttc ccgccgagcg catccacgcg gtctacgtga cgccctgccc
     3001 cgccaagatg gtgtccatcg tggaccatcc cggcatggag acgtcctaca tcgacg
//
LOCUS       DAOSIF010000386         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27899_length_3055_cov_3.754333, whole genome shotgun sequence.
ACCESSION   DAOSIF010000386 DAOSIF010000000
VERSION     DAOSIF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27899_length_3055_cov_3.754333"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLT35_11430"
     CDS             complement(<1..531)
                     /locus_tag="PLT35_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOG29879.1"
                     /translation="MALKDTLKQKIEEFRPRTAKLVKEHGKIVIDQVTIDQAIGGARD
                     VRCLVTDISYLDPQEGIRFRGKTIPETFEALPKAPGSNYPTVESFWYFLLTGDVPTDA
                     QVSEVVAEWKTRQVVPQYVFDVLRALPRDTHPMTMLSIGLLTLQKESKFFAYYNSGKF
                     NKMAAWEQVYEDASDIV"
     gene            complement(743..1366)
                     /locus_tag="PLT35_11435"
     CDS             complement(743..1366)
                     /locus_tag="PLT35_11435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG29880.1"
                     /translation="MALTREELTARAAHFWRRTAHYRGFGYDRVAASEAIVAVAGPLD
                     APVLDVGTGMGLAARALAARGLEVVSVDTNAEDQQVAAALTDDPALLGRIQFTLADAA
                     RLPFAAGRFGSVVTVDVLHHLDDGSAVLAEIVRILRPGGTLVLSDFSREGFDLVARVH
                     ALEGRVHPEGPVTMAWAREHARGLGLIERAAFVTCLHDVSVFRAAAA"
     gene            complement(1476..2195)
                     /gene="prxU"
                     /locus_tag="PLT35_11440"
     CDS             complement(1476..2195)
                     /gene="prxU"
                     /locus_tag="PLT35_11440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930177.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1941..1943),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOG29881.1"
                     /translation="MADPPAGCARTTGSVVGATPAAPSASEAPTPQKEAPRMSARVGQ
                     KAPDFQAPAYYKGGFTTVKLSDYLGKWVLLCFYPGDFTFVUATEVSAVADKYATLQEL
                     GAEVLSISVDSHFVHKMWNDHELVKMVDGGIPFHMVSDQAGNVGRAFGVWDEAQGIEL
                     RGRFIIDPDGVIQAMEVLTPPVGRKFGETVRQLQAFQHVRASKGTEATPAGWEPGQPA
                     LKPGPDLVGNVWKVWQPKMEK"
     gene            2394..2894
                     /gene="tsaA"
                     /locus_tag="PLT35_11445"
     CDS             2394..2894
                     /gene="tsaA"
                     /locus_tag="PLT35_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOG29882.1"
                     /translation="MAIRAIGRIRTPFAEAAGAPIQPAYAQGIEGRVVLDEAFGPALD
                     DLEGFERIWLIYWMDRSLPYRLRVVPYRDRRERGLFATRAPSRPNPIGLSVVRLVRRE
                     GAVLHVTDVDMLDGTPLLDIKPYVPAFDACPLSRAGWLDAGAANRERADGRFHGDSGR
                     TPRRRS"
BASE COUNT          446 a         1079 c         1046 g          484 t
ORIGIN      
        1 cacgatgtcg gaagcgtcct cgtagacctg ctcccaggca gccatcttgt tgaacttgcc
       61 cgagttgtaa taggcgaaga acttcgactc cttctgcagc gtcaggaggc cgatcgacag
      121 catggtcatc gggtgcgtgt cgcgcggaag cgcccgcagg acgtcgaaca cgtactgcgg
      181 caccacctgc cgcgtcttcc actccgcgac gacctcgctc acctgcgcgt cggtcggcac
      241 gtcgcccgtg agcaggaagt accagaacga ctccacggtc gggtagttgg atccgggcgc
      301 cttcggcagc gcctcgaacg tctcgggaat ggtcttgccc ctgaaacgga tgccttcctg
      361 agggtcaagg taggagatgt cggtgacgag gcaccggaca tcgcgcgccc cgccgatggc
      421 ctgatcaatc gtcacctggt cgatgacgat cttgccatgc tccttgacga gcttcgcggt
      481 ccgggggcgg aactcctcga tcttctgctt cagggtgtct ttcagggcca tgcgtccact
      541 ctcctcgacg gaatgagaca ttagggggga ccacagagcg ggggactccg cccatgttac
      601 accacgtgtc gacatccgtc taggacgggc agccgagcgg cgcgtcttcc gccgctgacg
      661 gaaagtcccc gacgagccgc ctgttaggag cgtgcacgcc gcggcgcggg agcggcccgc
      721 cgggcggccg cgccctggcc cgtcacgcgg ccgccgcccg gaagacgctc acatcgtgca
      781 ggcaggtgac gaaggctgcg cgttcgatga gccccaggcc ccgcgcatgc tccctggccc
      841 acgccatcgt caccggcccc tccgggtgga cgcgcccttc cagggcatgg acgcgcgcga
      901 ccagatcgaa cccctcgcgg gagaagtccg acaggacgag cgtgcccccg ggccgaagga
      961 tccgaacgat ctcggcgagc acggcggagc cgtcgtcgag atgatgcagg acgtccaccg
     1021 tcactacgga accgaagcgc ccggcggcga acggcaggcg ggccgcgtcg gcgagggtga
     1081 actggatgcg cccgagcagg gccgggtcgt cggtcagggc cgccgccacc tgctgatcct
     1141 cggcgttcgt gtccacggaa acgacctcga ggccgcgcgc cgccagcgcc cgggccgcga
     1201 gccccatgcc ggtgccgaca tcgagcacgg gagcgtcgag agggcccgca acggccacga
     1261 tcgcctccga cgcggccacg cggtcgtagc cgaagccgcg gtagtgggcc gtgcggcgcc
     1321 agaagtgcgc ggcgcgagcg gtcagctctt cacgcgtcaa ggccacggcg catgctccgg
     1381 gcgtgcgggg cgccggtctg cgtccggagc gcccattaca cgacagggcc ggcgcgagcc
     1441 ggccctgtcc cctgtaccgc gtggcgcgca gcctctcact tctccatctt cggctgccac
     1501 accttccaca cgttgccgac gaggtcgggc cccggcttga gggcgggctg gcccggctcc
     1561 cagccggccg gcgtcgcctc cgtacccttg ctggcgcgca cgtgctggaa cgcctggagc
     1621 tgacggaccg tctcgccgaa cttgcggccc acggggggcg tcagcacttc catcgcctgg
     1681 atgacgccgt cgggatcgat gatgaacctc ccgcggagct cgatgccctg ggcctcgtcc
     1741 cacacgccga aggcccggcc cacgttgccg gcctgatcgg acaccatgtg gaagggaatg
     1801 ccgccgtcca ccatcttcac cagctcgtga tcgttccaca tcttgtggac gaagtggctg
     1861 tccacgctga tcgagagaac ctcggcgccc agctcctgga gcgtcgcgta cttgtcggcg
     1921 accgccgaca cttcggtcgc tcaaacgaag gtgaagtcac cggggtagaa gcagagcagc
     1981 acccacttgc cgaggtagtc cgagagcttg acggtcgtga atccgccctt gtagtaggcc
     2041 ggagcctgga agtccggggc tttctgtccg acgcgggcac tcatgcgcgg ggcctccttc
     2101 tgtggtgtcg gcgcttccga tgctgatggg gccgccggcg tggcgccgac gaccgatccc
     2161 gttgtccgtg cgcatccggc gggtgggtcc gccatctgac ctcctgcgtg aagagagcgc
     2221 gttgacgatg aggggctgtt cctccacgag cataccgcgg ccgccgacag tgtcaagatc
     2281 gcgcggctcg tctcgggcct gcgccgggcg gccccgccag accgtgccgc cggccctcgc
     2341 gcctgcggta gcatacaggc gtggcacgct gtggtcggga ttcggcgccg cgcatggcca
     2401 tccgggcgat cggacgcatc cgcaccccgt ttgccgaagc ggcaggcgcg cccattcagc
     2461 ccgcgtacgc ccagggcatc gagggccggg tcgtcctgga cgaggccttc gggcccgcgc
     2521 tggacgatct ggagggcttc gagcggatct ggttgatcta ctggatggac cggtcccttc
     2581 cgtaccggct ccgcgtcgtt ccgtaccgcg accggcggga gcgagggctc ttcgccacgc
     2641 gcgccccgag ccggcccaac ccaatcggcc tctcggtcgt caggctggtg cggcgggagg
     2701 gcgccgtcct gcacgtcacc gacgtcgaca tgcttgacgg cacgccgctg ctcgacatca
     2761 agccgtacgt ccccgctttc gacgcctgcc cgctgtcaag agcgggctgg ctggacgccg
     2821 gcgccgccaa tcgcgaacgc gccgacgggc ggttccacgg cgacagcggc cgaacaccgc
     2881 ggaggcgctc atgacgctgc tgctgatcct gttgtcgtcg ctcgtcgggg gcgtcgtctc
     2941 ggtcctgctg gcggcgaccg tgctcgtggt gcggacgtct ttcgtggacc gcgcgcttcc
     3001 gcgcctcatc agcttctcca cgggcgcgct gctcggcgcc gcgatgctgg gcatg
//
LOCUS       DAOSIF010000387         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27915_length_3054_cov_3.570190, whole genome shotgun sequence.
ACCESSION   DAOSIF010000387 DAOSIF010000000
VERSION     DAOSIF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27915_length_3054_cov_3.570190"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3054)
                     /locus_tag="PLT35_11450"
     CDS             complement(<1..>3054)
                     /locus_tag="PLT35_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG29883.1"
                     /translation="NLPPGTYDVKASLEGFRELVRTGYAVGAGQVIRADLMLQIGQLA
                     ETVNVVAESTLLQTEKADLNTEISSKAVVNLPLNQWRNYQALLNLVPGATPTQLQNAE
                     LDTPARSLRTWVNGTQPNANTTRVDGAVSVNVWLPHHAMYVQSAESIDTVNIATNSFD
                     ADTGMAAGAAQTVITKSGTNTLRGSAFYFLNNDSLTANTYYNKYFDLPKAAVDTKTLG
                     ATLGGPIVKNKLFYFASWERYDTKRPTTYTYSVPTAKMRAGDFSEVAAAYPTFKLFNP
                     FSDLTGAAREQWTDNIIPSQYLNPIAQKLLRFLPAVNSTKDLNSNLLLDDYTQLRTEF
                     QTRDNYDVKLNYQITPNAMVWGKFGYMKNKGSGNNFYLGFDNPPSGDTKVYLTTFGTT
                     WTLNSTTVLDANIGMSRQNQDLFPPDYGTNYGLEAGIPGVNNPNDIRESGLPYMGATY
                     AVGAGTASWLPLFRKETSYSGSIGVTKVFTKHEMRVGFDFVRLELNHRQAEWGSYGLK
                     GGFGFSNNTTGAVGYTSPGWNSWAAYLMGLAQSYSEDTQTEDMSGRENQFAFYVRDRW
                     KVTPKLTVSAGARFDYYPLMERANGRGIEILNYTTRVVTLGGVGGQPKNPGLNYKAFY
                     IEPRLGAAYRLNEDTVFRAGYGITKNPLPWSRPMRGSYPFDVNNNANSLGTYDWVTTL
                     NQGIPLVKLPDTSTGQVILPSGVYIRSPNPDMVDRGTVQQWNVTVERRLPMALALEVA
                     YVGTATDGGYADLNVNVGRPGGGGSAAAYYSSFGTSAITDWGSRTKSRYKGLQVALNR
                     PFKNGLLLKGAYTWSQSKNMADEDGWTGLTWNYLPKYDDNFSIAGFDRTHVASFAWVY
                     ELPFLKDRTDAAGTILGGWQWNGVARWFTGTPYSIGGTNNAMACQGCGSILINYSGKP
                     EAIGKPSDFTAPYYDKGKFSQPSGLGLEGFGNTKRNHFRRPAQWALDMSLFKTFRVGR
                     FSPEFRLDVTNVFNHFNWGTPNTSITSSNFMLYNPGSVDYTAVFT"
BASE COUNT          441 a          948 c         1068 g          597 t
ORIGIN      
        1 gtgtgaagac cgcggtgtag tccacgctgc ccgggttgta gagcatgaag ttcgacgacg
       61 tgatcgacgt gttcggcgtg ccccagttga agtggttgaa cacgttggtc acgtccaggc
      121 ggaactccgg cgagaagcgg ccgaccctga atgtcttgaa gagcgacatg tcgagggccc
      181 actgcgccgg ccggcggaag tggttccgct tggtgttgcc gaagccctcg aggccgaggc
      241 cgctcggctg cgagaacttg cccttgtcgt agtacggcgc cgtgaagtcg ctcggcttgc
      301 cgatcgcttc gggcttgccg ctgtagttga tcaggatcga gccgcagccc tggcaggcca
      361 tggcgttgtt cgtgccgccg atgctgtacg gcgtgccggt gaaccaccgc gccacgccgt
      421 tccactgcca gccgccgagg atggtgcccg cggcgtcggt gcgatccttg aggaacggca
      481 gctcgtagac ccaggcgaag ctggccacgt gcgtccggtc gaagccggcg atggagaagt
      541 tgtcgtcgta cttcggcagg tagttccagg tcaggcccgt ccacccgtct tcgtcagcca
      601 tgttcttgga ctggctccag gtgtacgcgc ccttgagcag gaggccgttc ttgaacggcc
      661 ggttgagggc gacctgcagc cccttgtacc ggctcttcgt ccgcgagccc cagtccgtga
      721 tggccgacgt gccgaacgag ctgtagtagg ccgcagcgct gccgccgccg cccggccggc
      781 cgacgttcac gttcaagtcg gcgtagccgc cgtcggtcgc cgtgcccacg taggcgacct
      841 cgagggcgag ggccatcggg agccggcgct caaccgtgac gttccactgc tggaccgtgc
      901 cgcggtccac catgtccggg tttggcgagc ggatgtacac gccgctcggc aggatcacct
      961 ggccggtgga ggtgtcgggg agcttcacga gcgggatgcc ctgattcagc gtggtgaccc
     1021 agtcgtaggt cccgagggag ttggcgttgt tgttgacgtc gaacgggtag gatccgcgca
     1081 tcgggcgcga ccacggcagc gggttcttcg tgatgccgta gccggcgcgg aacacggtgt
     1141 cctcgttgag gcggtacgcc gcgcccaggc gaggctcgat gtagaacgcc ttgtagttga
     1201 gaccggggtt cttcggctgg ccgccgacgc cgccgagcgt cacgacccgc gtggtgtagt
     1261 tgaggatctc aatgccccgg ccgttggccc gctccatgag cgggtagtag tcgaagcggg
     1321 cgccggcgct gaccgtcagc ttcggcgtca ccttccagcg atcgcggacg tagaaggcga
     1381 actggttctc gcgccccgac atgtcctcgg tctgggtgtc ctcggagtac gactgcgcca
     1441 ggcccatcag gtacgcggcc cagctgttcc agcccggcga cgtgtagccc acggcgcccg
     1501 tggtgttgtt cgagaacccg aagccgccct tgaggccgta cgagccccac tccgcctggc
     1561 ggtggttgag ctccagccgc acgaagtcga agcccacgcg catctcgtgc ttcgtgaaga
     1621 ccttcgtcac gccgatggag ccggagtagc tggtctcctt acggaagagc ggcagccagc
     1681 tcgccgtccc ggcgcccacg gcgtaggtgg cgcccatgta gggcagaccg ctctcgcgga
     1741 tgtcgttcgg gttgttcacg cccgggatgc cggcctcgag gccgtagttc gtcccgtagt
     1801 caggcgggaa caggtcctgg ttctggcggc tcatcccgat gttggcgtcg agcaccgtcg
     1861 tcgagttgag cgtccaggtg gtgccgaagg tcgtcaggta gaccttggtg tcgccgctcg
     1921 gggggttgtc gaacccgagg tagaagttgt tcccagagcc cttgttcttc atgtagccga
     1981 acttgcccca gaccatcgcg ttcggcgtga tctggtagtt cagcttgacg tcgtagttgt
     2041 cgcgggtctg gaattccgtg cggagctgcg tgtagtcgtc gaggaggagg ttcgagttga
     2101 ggtccttggt gctgttcacc gccggcagga acctgagcag tttctgcgcg atcggattca
     2161 ggtactgcga cgggatgatg ttgtccgtcc actgctcccg cgcggcgccg gtcagatccg
     2221 agaacgggtt gaacagcttg aaggtcggat acgccgccgc cacctcggag aagtcgcctg
     2281 cgcgcatctt cgcggtcggg acggagtagg tgtacgtcgt cgggcgcttc gtgtcgtagc
     2341 gctcccagga cgcgaagtag aacagcttgt tcttcacgat gggcccgccg agcgtggcgc
     2401 ccagggtctt cgtgtccact gccgccttcg gcaggtcgaa gtacttgttg tagtaggtgt
     2461 tcgccgtgag gctgtcgttg ttcaggaagt agaacgcgga gccccgcagc gtgttcgtgc
     2521 ccgacttggt gatgacggtc tgggcggcgc ccgccgccat gcccgtgtcg gcgtcgaagc
     2581 tgttcgtcgc gatgttgacc gtgtcaatcg actcggccga ctgcacgtac atcgcgtggt
     2641 gcggcagcca cacgttcacc gacaccgcgc cgtccacgcg cgtggtgttg gcgttcggct
     2701 gcgtgccgtt gacccaggtg cgcagcgagc gcgccggcgt gtcgagctcg gcgttctgca
     2761 gctgggttgg cgtcgcgcca ggaacgaggt tgagcagcgc ctggtagttg cgccactggt
     2821 tcagcggcag gttgaccacc gccttggacg agatctcggt gttgagatcc gccttctccg
     2881 tctgcagcag cgtgctctcg gccacgacgt tgaccgtctc ggccagctgc ccgatctgga
     2941 gcatcagatc ggcgcggatg acctggccgg cgcccacggc gtacccggtc cggacgagct
     3001 cacggaaccc ctcgaggctc gccttcacgt cgtacgtgcc cggcggcagg ttgc
//
LOCUS       DAOSIF010000388         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27916_length_3054_cov_3.561187, whole genome shotgun sequence.
ACCESSION   DAOSIF010000388 DAOSIF010000000
VERSION     DAOSIF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27916_length_3054_cov_3.561187"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLT35_11455"
     CDS             <1..567
                     /locus_tag="PLT35_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29884.1"
                     /translation="FTYHSVLHGQPHVARVLVHAFRLIAATGFVDETPRLWAAVYLHD
                     LARTSDGLCPGHGADAWARLATLPAVQELFALGGVRPEDYPAIEAAVSLHSRGEARAG
                     TPEARLTKLLKDADGLDRVRFGDLDPDYLRHPEARGMIRYAQRLWGETVHKLPVGPGY
                     FARLWPEARRLAALVPDLKKPAPSKERE"
     gene            complement(592..1518)
                     /locus_tag="PLT35_11460"
     CDS             complement(592..1518)
                     /locus_tag="PLT35_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29885.1"
                     /translation="MTIWDDRYCTDAAPTNSTSKQAAVMRLARAEGLVEVRHAPFDAD
                     ATWADIATVHDRAYVEAVRTGSPRRLAESQGFRWSMAQADAVARIWNGHTAACRLAGA
                     AGLVLHPVSGAHHAGPAEGSGFCTFNFLSGAARGVLREGLRLVAIVDLDVHQGDGTYE
                     LEAGNPGVALFDIAGSRWADVSNGDRIEYRIVGDAESYREALQRLPAFLDRLHPDLVQ
                     YQAGMDPFEHDPIGGLDGVSEAFLTWRDAFVIGHVRRRGIPLVVNLAGGYSAGVSERL
                     HLNTVRVMAGRSGEDEPAGPPSATGAPERTET"
     gene            complement(1651..2787)
                     /locus_tag="PLT35_11465"
     CDS             complement(1651..2787)
                     /locus_tag="PLT35_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29886.1"
                     /translation="MTRAPLLRARRLVHAALAAAAIGAAAAPLPRGAAADAPARTAVS
                     ISGDDFRINGAPTLRGVRWRGYRLEGLLPNSRMVQGVFDDLNSDTRARWAYPDTNAWD
                     PDRNTREFVAAMPEWRRHGLLAITLNLQGGSPEGYSGEQHWHNSAFAEDGSLRPDYMA
                     RAALILNEADRLGMAVILGLFYFGQDERLRDEAAVTRAVDEAVAWVHAMGYRNVLVEI
                     NNECDIRYDHGILQPDRVHELIVRAKRAAPGGGRLLVSTSYGGRTLPGSAVLAVSDFV
                     LLHGNGVTKPDRIAAMVRQTRQLPRYTPKPIVFNEDDHYGFDEPWNNFVAATSEHASW
                     GYFDYRRRGEGPDEGYQSVPVSWGISSARKRGFFTLLRGMTGGR"
     gene            complement(2857..>3054)
                     /locus_tag="PLT35_11470"
     CDS             complement(2857..>3054)
                     /locus_tag="PLT35_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29887.1"
                     /translation="TSSPLEAQADGEYVARVSRPFRGFTAYLVELTFPSGLAGAPFTF
                     TTGVKVVPDVEPFRDLVEGRK"
BASE COUNT          415 a         1116 c         1079 g          444 t
ORIGIN      
        1 ttcacgtatc acagcgtgct gcacggccag ccgcacgtgg cccgcgtgct ggtccacgcg
       61 ttccgcctga tcgcggcgac cgggttcgtc gacgagaccc ctcgcctgtg ggcggcggtg
      121 tacctgcacg acctcgcgcg aaccagcgac gggctgtgcc cgggccatgg cgcggacgcc
      181 tgggccaggc tcgcgacgct gcccgccgtg caggagttgt tcgcgctggg cggcgtccgg
      241 ccggaggact atccggccat cgaggccgcg gtcagcttgc acagccgagg cgaggcgagg
      301 gcgggaacgc ccgaggcccg cctgacgaag ctcctgaagg acgccgacgg gctcgaccgc
      361 gttcgattcg gggatctcga cccggattac ctgcgccacc ccgaagcccg cggcatgatc
      421 cgctacgccc agcggctctg gggcgagacg gtccacaagc tgcccgtcgg cccgggctac
      481 ttcgcccgcc tctggccgga ggcgcggcgg ctcgcggcgc tcgtgcccga cctcaagaag
      541 cctgctccga gcaaggagcg cgaatagccg ctcgccagtc gccgggtgcg atcaggtttc
      601 cgtccgttcg ggagccccag tcgcagacgg cgggccggcc ggctcgtcct cgccgctcct
      661 gcccgccatc acgcggaccg tgttcaggtg cagccgctcg gacacgccgg cgctgtagcc
      721 gccggcgagg ttgacgacga gcggaatgcc gcgccgccgc acgtgtccga tgacgaaggc
      781 gtcccgccac gtgaggaacg cctcgctcac cccgtcgagg ccgccgatcg gatcgtgttc
      841 gaacggatcc atgcccgcct ggtactgcac caggtccggg tggaggcggt cgagaaacgc
      901 gggcaggcgc tggagcgcct cacggtagct ttccgcgtcg ccgacgatgc ggtactcgat
      961 gcgatcgccg ttcgagacgt ccgcccaccg gcttcccgcg atgtcgaaga gcgccacgcc
     1021 cggattgccg gcctcgagtt cgtatgtgcc atcgccctga tgcacgtcca gatcgacgat
     1081 cgccacgagc cggagcccct cccgcagcac cccccgcgcg gcgccagaca ggaagttgaa
     1141 cgtgcagaag cccgagccct cggcggggcc ggcgtggtgc gcgccggaca ccggatgcag
     1201 gacgaggccc gcggccccgg cgaggcggca ggcggcggtg tggccgttcc agattcgcgc
     1261 gaccgcgtcg gcctgcgcca tggaccagcg aaagccctgg gattccgcga ggcgcctcgg
     1321 agaacccgtc cggaccgctt ccacgtaggc ccggtcgtgg accgtggcga tgtccgccca
     1381 cgtggcgtcg gcatcgaacg gggcatgccg gacctcgacc aggccctccg cccgcgccag
     1441 gcgcatcacg gcggcctgct tgctcgtcga gttcgtgggc gcggcgtccg tgcagtaccg
     1501 gtcgtcccag atcgtcacga cttgtgtgtg gctcgcggcg gacctcccgg tttcgagcgg
     1561 gggccctcgc ccccgtcagg accattatcc gatgccgctg tgacagggcg ggtcaaaggc
     1621 cgtgtcctcg tgccgccggg cgcagctccg ctaccggccg cccgtcatcc cgcggagcag
     1681 cgtgaagaac ccgcgcttgc gggccgacga gatcccccag ctcaccggga cgctctggta
     1741 gccctcgtcc gggccctcgc ccctcctgcg atagtcgaag tagccccacg acgcgtgctc
     1801 gctcgtggcc gccacgaaat tgttccacgg ttcgtcgaag ccgtagtgat cgtcctcgtt
     1861 gaagacgatg ggcttgggcg tgtagcgggg gagctggcgc gtctgacgca ccatcgccgc
     1921 gatgcggtcg ggcttcgtga cgccgttccc gtggagcagg acgaagtccg agaccgccag
     1981 caccgcgctt cccggcagcg tgcggccgcc gtagctggtg ctgaccagga gccgccctcc
     2041 gcccggcgcg gcccgcttcg cccgcacgat cagctcgtgg acccgatccg gctgcaggat
     2101 cccgtggtcg tagcggatgt cgcactcgtt gttgatctcg acgaggacgt tccggtagcc
     2161 catggcgtgc acccatgcca ccgcctcgtc caccgcccgg gtcaccgccg cctcgtcgcg
     2221 caggcgctcg tcttgtccga agtagaagag gccgaggatc accgccatgc ccaggcggtc
     2281 cgcctcgttc aggatcaacg cggcgcgggc catgtagtcg ggccgcagcg atccgtcttc
     2341 ggcgaacgct gagttgtgcc agtgctgttc ccccgagtag ccctcgggac tgccgccctg
     2401 caggttgagc gtaatcgcga gcagcccgtg ccggcgccac tcgggcatcg ccgccacgaa
     2461 ctcgcgggtg ttgcgatccg gatcccaggc gttcgtgtcc gggtaggccc agcgcgcccg
     2521 cgtgtccgag ttcaggtcgt cgaagacgcc ctgcaccatg cgcgagttcg gcagcaggcc
     2581 ctcgaggcgg tagccccgcc agcggacgcc ccgaagcgtc ggagcgccgt tgatccggaa
     2641 gtcgtcgccc gagatcgaga cggcggtgcg ggcgggggcg tcggcggccg ccccgcgcgg
     2701 cagaggggcg gctgcagccc cgatggccgc ggcggcgagg gccgcgtgca caaggcgccg
     2761 agcgcgcagc aggggcgctc gagtcatggt ggttttccgc ccttcccgcc gcggccgcgg
     2821 ccaggcgggc gtggccgcct cgggtctgtc cggcgcctac ttgcgccctt ccacgaggtc
     2881 gcggaacggc tcgacgtctg gcaccacctt cacgcccgtc gtgaacgtga acggcgcgcc
     2941 ggcgaggccg ctcgggaacg tcagctcgac gaggtaggcc gtgaagcctc ggaagggccg
     3001 ggacaccctc gccacgtact cgccgtccgc ctgggcctcg agcggcgaac tcgt
//
LOCUS       DAOSIF010000389         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_27968_length_3050_cov_3.938564, whole genome shotgun sequence.
ACCESSION   DAOSIF010000389 DAOSIF010000000
VERSION     DAOSIF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27968_length_3050_cov_3.938564"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>454)
                     /locus_tag="PLT35_11475"
     CDS             complement(<1..>454)
                     /locus_tag="PLT35_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29888.1"
                     /translation="GATIFTDAAAPFQATYLVPADSVVGSTLAVTARAADAAGNLSES
                     LLSILVSDAADTTPPVVSLTAAAETPPGALLTFAASASDGSGVALLTFAVDGAVIASL
                     SDPPYQVQYQVPVALVPGMTLQVTAAATDFAGLSAAEAKTVTVVAPSAS"
     assembly_gap    455..464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<465..2802)
                     /locus_tag="PLT35_11480"
     CDS             complement(<465..2802)
                     /locus_tag="PLT35_11480"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG29889.1"
                     /translation="MTVLPPAGGAVQGAGINCGLGGAVCSVTMPAAMTLGLTATPASG
                     YTFTGWTGDCLGSSRNLWVLLNGPRVCGAAFAPIGGPENQAPVITSTPGLTATVGQAY
                     GYLVTATDGDGDALTYALTAAPPGMTIGAATGLVTWTPAAGQAGAQAVTVAVSDGRGG
                     TATQSFTITVGTVPVENRAPQITSAPVTAATATEPYAYRVVATDADSDPLGFALTAAP
                     AGMTIGSATGLVAWTPAPGQAGSHAVSVLVSDGRGGTARQSFTIAVANAPNEAPAVVI
                     PPVPPVTLPATAAITASVTDDGQPQPSPTLVWEQIDGPGTATFSPANAATTTVGFSAP
                     GTYTLRLTANDGALTGSASCTITVNEAANQTPVVTIAPIPPVILPATATALATISDDG
                     KPQPSPTLTWSKSSGPGDVTFASPAAATTVVTFGAAGTYVLSLSAFDGAATGSGTVTV
                     TVMPPNEPPTVSAAGDASVVLPASAALRGIASDDGLTRALTYSWTKTSGPGTVTFSAA
                     NALETTAAFGAAGTYVLRFTADDGEYQRSADVPVTAAAPASSNAAPVVGAGPDLAVTL
                     PGTAALAGTATDDGKPAGSALDVTWLKVSGPGTVSFANAKEAATTAAFGEAGIYVLQL
                     TASDGELVTADEVVVTVSAASAAGDRSPPVVSLTAPATALPGAQVDVRAEAVDGTGVS
                     VLRLEIEGQAPVEVSGGSLSAVLDVPAVAAVGLTFRIVAIARDAAGNEGRAETFLTVT
                     ATPDTTPPEIRVRGPASAAPGQLVVLTAEASDAVGVASV"
     gene            2690..>3050
                     /locus_tag="PLT35_11485"
     CDS             2690..>3050
                     /locus_tag="PLT35_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29890.1"
                     /translation="MSPSVIAAGIVTEQTAPPSPQLMPAPWTAPPAGGSTVIAYGGPT
                     EGAPPDATNLALHVRIPLTCTVVVCREPAQSPPHPLKVKPLSGTAVKVAEVALANVTW
                     QAEPPVPHMRPPQFTVPP"
BASE COUNT          408 a         1144 c         1083 g          405 t
ORIGIN      
        1 tgacgccgaa ggcgcgacga ccgtcaccgt cttcgcttcc gctgccgaga ggccggcgaa
       61 gtcggtcgca gccgccgtca cctggagcgt catgcccggc acgagcgcca cgggcacctg
      121 gtactggacc tgatacggcg gatcggacag cgaggcgatg acggccccgt cgacggcgaa
      181 cgtgagcagg gcgaccccgc tcccgtcgct tgccgacgcc gcaaaggtca ggagcgcgcc
      241 gggcggcgtc tcggccgccg ccgtcagcga caccacgggc ggcgtcgtat ccgcagcgtc
      301 cgacacgagg atgctgagca ggctctcgct gagattgccg gccgcatcgg ccgcgcgcgc
      361 cgtgaccgcc agggtggacc ccacgaccga gtcggccggg accaggtacg tggcctggaa
      421 cggcgccgca gcgtcggtga agatcgtggc gccgnnnnnn nnnncgacgg acgccacgcc
      481 gaccgcgtcg ctggcctccg cggtgagtac gacgagttgg cccggggcgg cgctcgccgg
      541 ccctcggacg cgaatttcgg gaggcgtcgt gtcaggcgtg gccgtgacgg tgaggaacgt
      601 ctccgcgcgc ccctcgttgc ccgcggcgtc gcgcgcgatg gcgacgatcc ggaacgtcag
      661 gcccaccgcg gcgacggcgg gaacgtcgag taccgccgac aggcttcctc cgctgacttc
      721 cacgggcgcc tgcccctcga tctcgagccg aagcacggac acgcccgtgc cgtcgacggc
      781 ctcagccctg acgtcgacct gggcgccagg cagggccgtg gccggagccg tgaggctgac
      841 gaccggcggc gatcgatccc ccgcggccga cgccgccgac accgtcacca cgacctcgtc
      901 ggccgtcacc agttctccgt cgcttgccgt cagttgcagc acgtagatgc ccgcttcgcc
      961 gaacgccgcg gtggtcgcgg cctccttcgc gttcgcgaag ctcaccgtgc cggggccgct
     1021 caccttcagc cacgtcacat cgagcgcgct ccccgccggc ttgccgtcgt cggtggccgt
     1081 tcccgccagc gccgcggtgc cggggagcgt gaccgcgaga tccgggccgg cgccgacgac
     1141 cggcgcggcg ttcgacgagg ccggcgcggc cgcggtgacc gggacgtcgg ccgatcgctg
     1201 gtactcgccg tcatcggcgg tgaagcgcag gacgtaagtt ccggccgcgc cgaaggccgc
     1261 ggtcgtctcg agcgcgttcg cggccgagaa cgtcaccgtc cctggaccgc tcgtcttggt
     1321 ccacgagtag gtcagcgcgc gagtcagtcc atcgtccgag gcaattccgc ggagagctgc
     1381 agatgcgggc agcacgaccg acgcgtcacc cgccgcagag acagtgggcg gctcgttcgg
     1441 gggcatgacc gtcaccgtca ccgtccccga ccccgtcgcg gcgccgtcga acgcgctcag
     1501 cgacagcacg tacgtgccag ccgcgccgaa ggtcactacc gtcgtggcag cggcaggcga
     1561 tgcgaacgtc acgtcgcccg gcccgctgga cttcgaccac gtgagcgtgg gtgacggctg
     1621 cggcttcccg tcgtcggaga tcgtcgcgag cgccgtggcc gtcgccggca ggatcaccgg
     1681 cgggatcggc gcaatcgtaa ccaccggcgt ctggttcgcc gcctcgttca cggtaatcgt
     1741 gcaggacgcc gagcccgtca gcgctccgtc gttcgccgtg agccgcagcg tgtaggtgcc
     1801 gggcgcgctg aagccgacag tggtcgtcgc tgcgttggcc ggcgaaaacg tggccgttcc
     1861 cgggccgtcg atctgctccc acacgagcgt gggcgacggc tgcggctgcc cgtcatcggt
     1921 gacgctcgcg gtgatcgcgg cggtggccgg gagggtgacc ggcggcaccg gcgggatcac
     1981 gacggccggg gcctcgttgg gcgcgttcgc gacagcgatc gtgaagctct gcctggccgt
     2041 gccgccccgc ccgtcgctga ccagcaccga gacggcgtgt gagccggcct ggccaggcgc
     2101 aggcgtccac gcgaccagcc ccgtggccga cccgatcgtc atgccggcgg gcgccgcggt
     2161 cagcgcgaag cccagcgggt cgctgtcggc gtcagtggcg acaacccggt aagcgtaggg
     2221 ctcggtggcc gtggccgccg tgacgggcgc ggacgtgatc tgcggcgccc ggttctcgac
     2281 cggcacggtg cccaccgtga tggtgaagct ctgcgtcgcc gtgccgcccc gcccgtcgct
     2341 caccgcgacg gtcaccgcct gcgccccggc ctgccccgcc gccggcgtcc acgtgaccag
     2401 ccccgtggcc gccccgatcg tcatccccgg cggcgcggcc gtgagcgcat acgtcagcgc
     2461 gtcgccatcc ccgtccgtcg ccgtcaccag atagccatac gcctgcccga ccgtcgccgt
     2521 cagccccggc gtcgacgtga tcaccggcgc ctggttctcg ggaccgccga tcggcgcgaa
     2581 ggcggctccg cacacccgag gtccgttcag caggacccac aggttccggc tgctccccag
     2641 gcaatcgccc gtccaacccg tgaaggtgta tccgctggcg ggcgtcgccg tgagtcccag
     2701 cgtcatcgcc gccggcatcg tcaccgagca gaccgcgccg ccaagcccgc agttgatgcc
     2761 cgccccctgg acggcgccgc cagcaggcgg gagcaccgtc atcgcgtatg gcggcccaac
     2821 cgaaggcgcc ccgcccgacg cgacgaacct cgcgctgcac gtccggatcc cattgacctg
     2881 tacggtggtc gtatgcaggg agcctgcgca gtcgcccccc cacccgctga aggtgaagcc
     2941 gctgtccggc acggcggtca aggtggcgga ggtggcgctg gcgaacgtga cctggcaggc
     3001 cgagccgccc gtgccgcaca tgaggccgcc gcaattcacg gtgccgcccg
//
LOCUS       DAOSIF010000390         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28013_length_3047_cov_3.720588, whole genome shotgun sequence.
ACCESSION   DAOSIF010000390 DAOSIF010000000
VERSION     DAOSIF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28013_length_3047_cov_3.720588"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2991
                     /locus_tag="PLT35_11490"
     CDS             <1..2991
                     /locus_tag="PLT35_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG29891.1"
                     /translation="VAVVAFAAGIGVLAVLPTEFLSHMDQGEFAVRFQSAPGSSMAET
                     RGRLTQVLKALSESREIQFTYASIGAGESDTVRDATVFVKLVPRAERERTLRQFVHDT
                     RVRLEKIPGVQLWVLEESDAFRKPLQILIQGDDIATLKRYAAAVKRELYSVPGIVDIA
                     AAMENDLPEYRLTVDRERAAATGLGSSALASTVGVLVGGQVVSTYEDEQGEAINVRVR
                     LPESLRGDVRQVADLKVSVPSQTGTVLVPVADLVTFTRASSAAEISRRDLARQVTVDA
                     SLDGLPLGSAGRLAMAAASRVAMEPGYRMVLGGETEMMVESFQYMGEALVLAIIFVYL
                     ILAAQFESFVDPLAIMLSLPLSVVGMAGMLALTGDTISLMSLIGLIMLMGLVTKNAIL
                     LVDYTKVLRRRGLDRRAALITAGRTRLRPILMTTSAMIFGMLPLALALGEGGEFRAPM
                     ARGVIGGLITSTLLTLIVVPVVYSVLDDVVGWLFRRRKQAASVAATAVLAAVLVLGPA
                     ARTSEAQAPRLAAPGPAAPAHAASARTAAADAAPAAAARLADAVAQAARTTPPAGVRV
                     LTLAQALEIAAAQNRDIQKAREYKKWVQGKYEEERASALPTIALTGSLTRYADNSQSR
                     LFSNLMPASEGGGDHVGFGDVFGGATSVRSASVTINQTIFTWGQVGAAIRAAKLGFDY
                     SGHQLRRFQQAVAKDVTTAFYNILAAREAVTIAEEDLAQKTRHLDEATKRHAAGTATD
                     YDILAAQVAVENARPAVIRAQNAVRATREQLRFLLAEPGDVDAEGTLATAVEPLPSYD
                     DVLATALDNRPELGEMQSQQGVYAELIKIASAAGKPRVDFAAAWGQQRLAISTLSGTG
                     STWNMAIVASVPIFDGWRTKGRVAQARSSRGSLDIDALKLREAISLEVRTAVDNAREA
                     GDLLTAASGPVTQAERLLALAEKGYELGVKTRLDVQDAELNLQAARANLAAAQRGYRV
                     ARVNLDWVAGVLAGGAPPAAQPGK"
BASE COUNT          429 a         1107 c         1102 g          409 t
ORIGIN      
        1 gtcgccgtcg tcgccttcgc ggccggcatc ggggtgctcg ccgtgctgcc gaccgaattc
       61 ctgtcgcaca tggaccaggg cgagttcgcc gtcaggttcc agagcgcgcc cggctcgtcc
      121 atggctgaga cgcgcggccg gctcacgcag gtgctgaagg ccctctccga atcgagggag
      181 atccagttca cctacgcgtc catcggcgcg ggcgagtccg acacggtgcg cgacgccacg
      241 gtcttcgtga agctcgtgcc cagggccgag cgcgagcgga cgctccggca gttcgtgcac
      301 gacacccgtg tgcgcctcga gaagatcccc ggcgtgcagc tctgggtgct ggaggagtcg
      361 gacgcgttcc ggaagccgct ccagatcctg atccagggcg acgacatcgc gacgctgaag
      421 cggtacgcgg ccgccgtcaa gcgcgagctg tactccgtcc ccggcatcgt ggacatcgcg
      481 gccgcgatgg agaacgacct gcccgagtac cgcctgaccg tggaccgcga gcgcgccgcc
      541 gcgaccggcc tcggcagcag cgccctcgcc agcaccgtcg gcgtgctcgt cggcggccag
      601 gtcgtctcga cctacgagga cgagcagggc gaggccatca acgtgcgggt gcgcctgccc
      661 gagtctctgc gcggcgacgt gcgccaggtg gccgacttga aggtgtcggt gccctcgcag
      721 accgggaccg tcctcgtgcc cgtggccgac ctggtcacgt tcacgcgcgc ctcgtcggcg
      781 gccgagatct cccggcgcga tctggcgcgg caggtcaccg tggacgccag cctggacggc
      841 ctgcccctgg gcagcgccgg gaggctggcc atggccgccg cgtcgcgcgt ggcgatggag
      901 cccggctacc gcatggtcct cggcggcgaa accgagatga tggtcgagtc gttccagtac
      961 atgggcgagg ccctcgtgct ggcgatcatc ttcgtgtacc tcatcctcgc ggcccagttc
     1021 gagtccttcg tcgatccgct cgcgatcatg ctctcgctgc cgctctccgt cgtcggcatg
     1081 gcgggcatgc tggccctcac cggcgacacg atcagcctca tgtccctgat cggcctcatc
     1141 atgctgatgg ggctggtcac gaagaacgcc atcctgctgg tggactacac caaggtgctg
     1201 cgacggcgcg gactggatcg gcgcgccgcc ctcatcaccg cggggcggac gcggctgcgg
     1261 ccgatcctga tgaccacctc ggccatgatc ttcgggatgc tcccgctggc cctggccctc
     1321 ggcgagggcg gcgagttccg cgccccgatg gcgcgcggcg tcatcggcgg cctgatcacc
     1381 tccaccctgc tgaccctgat cgtggtgccg gtcgtctact ccgtgctcga cgatgtcgtg
     1441 ggatggctct tccggcggcg gaagcaggcc gcctccgtcg cggcgacggc cgtcctggcg
     1501 gcggtgctgg tcctcggccc ggcggcgagg acgtcggagg cgcaagcgcc ccgactggcg
     1561 gcccctggcc cggcggcgcc ggcccatgcg gcatctgccc gcacggcggc ggccgacgcc
     1621 gcaccggctg ccgcggcgcg gctggccgac gccgtcgcgc aggcggcgag aaccacgcct
     1681 ccagcgggcg tccgcgtgct gacgctggcg caggccctgg agatcgcggc ggcgcagaac
     1741 cgcgacatcc agaaggcgcg cgagtacaag aagtgggtgc agggcaagta cgaggaggag
     1801 cgcgcctccg ccctgcccac gatcgccctg accggatcgc tgacgcgcta cgcggacaac
     1861 agccagagcc ggctcttctc gaacctcatg ccggcgtccg agggcggcgg cgaccacgtc
     1921 ggcttcggcg acgtcttcgg cggcgcgacc tccgtgcgct ccgcctcggt cacgatcaac
     1981 cagaccatct tcacctgggg ccaggtgggc gccgccatcc gcgcggcgaa gctgggcttc
     2041 gactacagcg gccaccagct gcgccgcttc cagcaggccg tcgccaagga cgtgaccacc
     2101 gcgttctaca acatcctggc cgcgcgcgag gccgtcacga tcgccgagga ggatctcgcg
     2161 cagaagaccc ggcacctcga cgaggccacc aagcggcacg ccgccggcac ggcgaccgac
     2221 tacgacatcc tggccgcgca ggtcgcggtc gagaacgcca ggcccgccgt catccgcgcc
     2281 cagaacgcgg tgcgcgccac cagggagcag ctgcggttcc tgctggccga gcccggcgac
     2341 gtcgatgccg agggcacgct cgccaccgcg gtcgagccgc tgccgtccta cgacgacgtg
     2401 ctggcgacgg cgctcgacaa ccggcccgaa cttggcgaga tgcagtccca gcagggcgtg
     2461 tacgcggagc tcatcaagat cgccagcgcc gcgggcaagc cgcgggtgga cttcgcagcg
     2521 gcctgggggc agcagcgcct ggcgatctcg acgctctcgg gaaccggcag cacgtggaac
     2581 atggcgatcg tcgcatcggt gccgatcttc gacggctggc gcacgaaggg gcgcgtggcc
     2641 caggcgcgga gcagccgggg cagcctggac atcgacgccc tgaagctgcg cgaggccatc
     2701 tcgctggagg tccggacggc cgtcgacaac gcgcgcgagg ccggggacct gctgacggcg
     2761 gccagcgggc ccgtcacgca ggcggagcgc ctcctcgcgc tggcggaaaa gggctacgag
     2821 ctgggcgtga agacgaggct ggacgtgcag gacgccgagt tgaacctcca ggccgcgcgc
     2881 gccaatctcg ccgcggcgca gcgcgggtac cgcgtggccc gggtcaatct ggactgggtc
     2941 gcgggcgtgc tggccggcgg cgcgccgccg gccgcgcagc ccgggaagta gaaggaccgg
     3001 cgcgcgccgc gtgccggccg ggcggggccg ccgcctgcca cgcgtgc
//
LOCUS       DAOSIF010000391         3044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28041_length_3044_cov_3.240214, whole genome shotgun sequence.
ACCESSION   DAOSIF010000391 DAOSIF010000000
VERSION     DAOSIF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28041_length_3044_cov_3.240214"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLT35_11495"
     CDS             complement(<1..632)
                     /locus_tag="PLT35_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG29892.1"
                     /translation="MSPRRKTSEESLLDIIERTPAPRGASWPPVGSWPPLPGSEQPGG
                     RGGGGGEQTVALHEAAQEKYLNYALSVITSRALPDVRDGLKPVQRRILFTMWQQSLTA
                     DAKFRKCAKVVGDVMGSYHPHGDSAIYDTLVRMAQPFSLRYPLVDGSGNFGSLDGDAA
                     AAMRYTECRLARISDEMLQEIDQATVTFRPNYDGTKTEPVVLPARLPNLLV"
     gene            complement(714..1391)
                     /locus_tag="PLT35_11500"
     CDS             complement(714..1391)
                     /locus_tag="PLT35_11500"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOG29893.1"
                     /translation="MAVPPFEELLAVVARGLARLPGAAAQNRMAPVPRPGGQRPRVTG
                     GARPAAALLLLFPADGVSASLLLTKRSQALVQHPGQVSLPGGAVNAGESIEDAAMREA
                     REETGIEPGSVRTLGRLTPLHIPVSGFVLHTVVGAASARPVVRPAPWEVERIIEAPVE
                     HLLDSARHLRTTRVRDGIELDMPFFELDGEQVWGATAMVLAEFVALLGVAVSPAAAKR
                     GRDGLRN"
     gene            complement(1442..>3044)
                     /locus_tag="PLT35_11505"
     CDS             complement(1442..>3044)
                     /locus_tag="PLT35_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG29894.1"
                     /translation="VYAIDLRTGERKLAVRKLRNMGQPSPDGSKFFYYDDGHYFSYDM
                     ASGASVNLTKSVPASFIDADQDTNVVKPPTPALGWTKDSDAVLLSDGWDIWRVPVSGG
                     TASNLTANGRRDGLRYRSLLRLDPEEKGFDLSAPRYVSMFGEATKKGGIGLIEPGQAG
                     VRRLLWDDAVFGLQKAAKADVYLYTRSTFNEPPDVHATGASLQPGRRLSDLRAQVGAF
                     TWSGGSLLVSYAVKLGKGAPAKTLQGSLFLPAGYERGRTYPTIVYIYERLTQNHNAFA
                     APTANGFNKSVYTSNGYAVLMPDIAYALNDPGVSAVACITSALKAAIATGVVDPKRVA
                     LHGHSWGGYQTAFAVTQTQAFAAAVAGAPLTNMVSMYSLIYKNSGGTNQAIFESSQGR
                     FLGGYWDNWEAYVRNSPVNFARNVKTPLIILHNDQDGAVDFTQGVEYFNTLRRLGKNV
                     IMLEYVGENHGLAKQANRQDYTIRMKEFFDHYLKGAPAPAWMIEGVPRLKMAEHLKER
                     ADLRKSAAEREKERAALPVREAERK"
BASE COUNT          467 a         1066 c          999 g          512 t
ORIGIN      
        1 accaggaggt tcggcaggcg cgccgggagg acgaccggct cggttttcgt gccgtcgtag
       61 ttcgggcgga aggtgacggt ggcctgatcg atctcctgca gcatctcgtc cgagatccgc
      121 gcaaggcggc attcggtgta gcgcatcgcg gcggccgcgt caccgtcgag ggagccgaag
      181 ttgcccgagc cgtccaccaa cgggtaccgg agcgagaacg gctgcgccat gcggaccagc
      241 gtgtcgtaaa tggccgagtc gccgtgcggg tggtagctgc ccatcacatc gcccacgact
      301 ttcgcgcact tgcggaactt ggcgtcggcc gtgaggctct gctgccacat cgtgaacagg
      361 atgcggcgct ggacgggctt gaggccgtcg cgcacgtcgg gaagtgcccg cgacgtgatg
      421 acggacaagg cgtagttgag atacttctcc tgcgccgcct cgtggagcgc gaccgtctgt
      481 tcaccgccac caccgccgcg gccaccgggc tgttcggagc ccggcagcgg aggccagctt
      541 ccaacgggcg gccacgaggc gcctctcggc gcgggggttc gctcgatgat gtccagcagc
      601 gactcttccg atgtcttccg acgaggtgac aacggtctgt tactcctcct gcgatcgaga
      661 gggagggggt cgggacggtt tacgcaaccg acgaaaagtg aaacacagtt ctgttagttg
      721 cgtaaaccgt cccgacccct tttggctgcc gcggggctga ccgccacgcc gagcagcgcc
      781 acgaactccg ccagaaccat cgccgtcgca ccccagacct gttcgccgtc gagctcgaaa
      841 aacggcatat cgagctcgat gccgtcgcgc acgcgggtcg tccgcaggtg gcgcgccgag
      901 tcaagcaggt gctcgacggg cgcctcgatg atccgctcca cctcccaggg agcggggcgc
      961 accaccggcc gcgcactcgc ggcgccgacc accgtatgga gcacgaatcc actcaccggg
     1021 atgtgcagag gcgtcagacg gcccagcgtc cggaccgatc ccggctcgat gccggtttcc
     1081 tcgcgcgctt cccgcatggc ggcgtcttcg atcgactcgc ctgcgttcac cgccccgcca
     1141 ggcagcgaca cctggccggg atgctgcacc agggcctgcg aacgcttggt cagaagcagc
     1201 gacgccgaca cgccgtccgc cgggaagagc agcagcaggg ctgccgcggg ccgcgccccg
     1261 ccggtcaccc tcggccgctg cccgccaggc ctgggaaccg gcgccatccg gttctgggcc
     1321 gcggcgcccg gcagcctcgc cagtccccgc gcgaccaccg caagcagttc ctcgaatggc
     1381 ggcacagcca tcggttcatg ctaccgcggg catcgggcgg accaaaaccc catgctccaa
     1441 cctacttcct ctccgcctcc ctcacgggca gtgccgcccg ctccttctcg cgctccgcgg
     1501 cggactttct aaggtccgcg cgctccttga ggtgctccgc catcttcagc cgcggcacgc
     1561 cctcgatcat ccacgcgggc gcaggcgcac ccttgaggta gtggtcgaag aactccttca
     1621 tgcggatcgt gtagtcctgc cggttcgcct gcttcgcgag gccgtggttc tcgccgacgt
     1681 actccagcat gatgacgttc ttgccgaggc gcctgagcgt gttgaagtac tcaacgccct
     1741 gcgtgaagtc cacggccccg tcctggtcgt tgtgcaggat gatgagcggc gtcttcacgt
     1801 tccgggcgaa gttgaccgga gaattccgga cgtaggcctc ccagttgtcc cagtaccccc
     1861 cgaggaagcg gccctggctg ctctcgaaaa tcgcctggtt cgtgccgccg ctgttcttgt
     1921 agatcaggct gtacatgctg accatgttcg tgagcggcgc gcccgcgacc gccgccgcga
     1981 acgcctgggt ctgcgtcacg gcgaaggccg tctggtagcc gccccaggag tgcccgtgca
     2041 gcgcgacgcg cttcggatcc actactccgg tcgcgatggc ggccttgagc gccgacgtga
     2101 tgcacgccac ggccgagacg ccgggatcgt tcagcgcgta ggcgatgtcc ggcatcagca
     2161 cggcgtagcc gttgctcgtg tacaccgact tgttgaagcc gttcgccgtg ggcgccgcga
     2221 acgcgttgtg gttctgcgtc aaccgctcgt agatgtagac gatggtgggg tacgtccggc
     2281 ccctctcgta accggccggc aggaacagcg agccctgcag cgtcttggcc ggggccccct
     2341 tgccgagctt cacggcatag ctcacgagca gcgagcctcc ggaccacgtg aaggcgccca
     2401 cctgcgcccg cagatccgag agcctccgcc ccggctggag tgaggcgccg gtcgcatgga
     2461 cgtccggcgg ctcgttgaag gtggatcgcg tgtacaggta tacgtcggcc ttggccgcct
     2521 tctgcagtcc gaacacggcg tcgtcccaga ggagccgccg cacgccggct tgcccgggtt
     2581 cgatgagccc gatgccgcct ttcttcgtcg cctcgccgaa catcgacacg tagcgcggcg
     2641 ccgagaggtc gaaccccttc tcctccgggt ccagccggag cagcgaccgg tagcggaggc
     2701 cgtccctccg gccgttcgcc gtcaggttcg acgccgttcc gccggacacg ggcacccgcc
     2761 agatgtccca gccgtcggag agcagcacgg cgtcgctgtc cttcgtccag cccagggcag
     2821 gcgtcggcgg cttgacgacg ttcgtgtcct gatcggcgtc gatgaacgac gccgggacgc
     2881 tcttcgtgag attcacggac gcacccgacg ccatgtcgta cgagaagtag tggccgtcgt
     2941 cgtagtagaa gaacttcgag ccgtcggggg acggctgtcc catgttccgc agcttcctga
     3001 ccgccagctt ccgctcgccc gttcgaaggt cgatggcgta caca
//
LOCUS       DAOSIF010000392         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28204_length_3033_cov_3.661854, whole genome shotgun sequence.
ACCESSION   DAOSIF010000392 DAOSIF010000000
VERSION     DAOSIF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28204_length_3033_cov_3.661854"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLT35_11510"
     CDS             <1..378
                     /locus_tag="PLT35_11510"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29895.1"
                     /translation="ACHFIGLPEGTTALTQAAVYLSTAPKSNAVYQAYLRAAADAHTD
                     RADPVPLHLRNAPTRLMKALDYGKGYQYAHDDPDAVTGMDCLPPAVAGRKYYTPTERG
                     FEKEIKRRLDGWAAIRKRRHRQG"
     gene            complement(411..2216)
                     /gene="mutL"
                     /locus_tag="PLT35_11515"
     CDS             complement(411..2216)
                     /gene="mutL"
                     /locus_tag="PLT35_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG29896.1"
                     /translation="MPRIHQLPPDLASQIAAGEVVERPASVVKELVENAIDAGAARIT
                     VAVELGGKALVRVEDDGEGMDADDARLALERHATSKIARAEDLEAIETMGFRGEALPS
                     IASVSRLVVRTRRRGAVAGTEVRVNGGVVASVTEVGMAPGTIVEVADLFYNLPARRKF
                     LKSDTAETAQVSRLASQLALAHPHVGFTVASGPRVLLRCPPAATLADRFYQIYGDRPD
                     LVPLVKDAGGIRVHGFVAALAEPGPTRGAQQFFVNRRIVKDRTVAHAVIDAYASATAR
                     ERRPEAHVFIEMPPDAVDVNVHPTKAEVRFRDQSLVHEIVRRAVQEAIGGVSAPELRL
                     SPPEAEAAGARAISLPGLLAGWYPRSPRAVEELPAADARRPAGEPGAASASFPGEAGG
                     GARLPGPAPDGSLGEIALKPLVPLGQFRNTFIIAVDTDGIAIIDQHVAHERVLYERIS
                     ARLLSGQLDSQRLLTPIVMDLPPGPRAALLAHGAELRRCGFEVEDFGLASVRVDAMPA
                     LLGREQGTAALKALADDLDGMGGAAPVEEHLRNMAATMACHAAVKANDPLTFEKMAHI
                     LEELRQTAYSTVCPHGRPVMLRISRREVEKNFQRT"
     gene            complement(2223..>3033)
                     /locus_tag="PLT35_11520"
     CDS             complement(2223..>3033)
                     /locus_tag="PLT35_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOG29897.1"
                     /translation="DLAVEDALQVVHHPKAETYDGYLYVILHGIDFKASQHEFATHDA
                     DFFVGPTYLVTVTDGTSRTIPEVQALCLRNPRVMAEGPMALMHRIVDTMVDHYRPEVD
                     KLEARIDELEDSVFEAAETRATNQEILDLKRDVASLRRIILPQRDILGRLGRREFPQV
                     SEEVSYRFRDVYDHVVRISDEALLFHDRLAAILEAHLSNVSNRLNEVMKILTIFSAVF
                     MPMTVLTGLYGMNVPLVHFPGGEPTQFWWILGIMAAAGAALLWLFRRWRWW"
BASE COUNT          447 a         1075 c         1079 g          432 t
ORIGIN      
        1 gcctgtcatt tcatcggcct gccggagggc accacggcgc tgacgcaggc cgccgtctac
       61 ctgtcgacgg ccccgaagag caacgccgtc tatcaggcgt acctccgtgc cgcggctgac
      121 gcccacaccg atcgcgccga cccggtgccg ctgcacctgc gcaacgcccc gacccggctc
      181 atgaaggcgc tcgactacgg caagggctat cagtacgccc acgacgaccc ggacgcggtc
      241 accggcatgg actgcctgcc gcccgccgtg gccgggcgga agtactacac gccgaccgaa
      301 cgtggcttcg agaaggaaat caagcgccgc ctcgacggat gggcggccat tcggaagcgg
      361 cggcacaggc agggctagcg cctctgggtc ccgggccccg actccggagc tcaggtccgc
      421 tggaagttct tctccacttc gcggcgggag atacgcagca tcaccggccg gccgtgcggg
      481 cacacggtgg agtaggcggt ctgccgcagc tcttccaaga tgtgcgccat cttctcgaac
      541 gtgagcgggt cgttggcctt caccgccgca tggcaggcca tggtggccgc catgttccgg
      601 aggtgctcct ccaccggtgc cgccccgccc atcccgtcga gatcgtcggc cagagccttc
      661 agcgccgccg tgccctgctc gcggccgagg agcgccggca tggcgtccac gcggacgctg
      721 gccagcccga agtcctccac ctcgaacccg caccgccgca actcggcgcc gtgcgcgagc
      781 agcgcggcgc gggggcccgg cggaaggtcc ataacgatcg gcgtgagcag gcgctggctg
      841 tcgagctgac ccgacagcag ccgggcggag atgcgctcgt agagcacccg ctcgtgcgcg
      901 acgtgctggt cgatgatggc gatgccgtct gtgtccacgg cgatgatgaa cgtgttgcgg
      961 aactggccga ggggcacgag cggcttcagc gcgatctccc cgagcgagcc gtccggcgcc
     1021 gggcccggca ggcgggctcc gcccccagcc tccccgggga aagaggccga cgccgccccg
     1081 ggctcgccgg ccgggcgacg cgcgtcggcc gccggcagtt cctccaccgc gcgcggactc
     1141 cgcgggtacc acccggccag gagccccggc agactgatgg cgcgcgcccc tgccgcctcg
     1201 gcctcaggcg gcgagagccg cagttccggc gccgagacgc cgccaatggc ctcctggacg
     1261 gcacggcgga cgatttcgtg cacgagagac tgatcgcgga agcgcacctc ggccttggtc
     1321 ggatggacgt tcacgtccac tgcgtcgggc ggcatctcga tgaacacgtg cgcctcgggc
     1381 ctccgctcgc gcgcagtcgc cgacgcgtac gcgtcgatga cggcgtgggc cacggtgcgg
     1441 tccttgacga tccggcggtt gacgaagaac tgctgggcgc cccgcgtggg ccccggctcg
     1501 gccagggccg ccacgaaccc gtgcacgcgg attccgcccg cgtccttcac gaggggcacg
     1561 agatccgggc gatccccgta gatctggtag aaccggtccg cgagagtcgc cgccggcggg
     1621 cagcggagca gcacgcgcgg cccgctggcg acggtgaacc cgacgtgcgg gtgggccagc
     1681 gcgagctggg aggcgaggcg ggacacctgg gccgtctctg cggtgtcgga cttcaggaac
     1741 ttccggcggg ccggcaggtt gtagaagagg tcggccacct cgacgatcgt ccccggcgcc
     1801 attccgacct cggtgacgga cgccaccacg ccgccgttga cgcgcacctc ggtgccggcg
     1861 accgcgccgc gccgccgggt ccgcacgacg aggcgcgaaa ccgacgctat gctgggcaag
     1921 gcctcgccgc ggaagcccat cgtctcgatc gcctcgaggt cttcggcgcg ggcgatcttg
     1981 ctcgtggcgt gccgctcgag ggcgaggcgc gcatcatccg cgtccatgcc ctccccgtcg
     2041 tcctcgaccc gcacgagcgc cttgcctccc agttcgactg cgacggtgat ccgcgcggcg
     2101 ccggcgtcga tggcgttctc gaccagttcc ttgacgaccg acgcggggcg ctcgacgacc
     2161 tcgccggcgg cgatctggct ggcgagatcg ggcgggagct ggtggatcct gggcatggcg
     2221 gcctaccacc agcgccagcg ccggaacagc cagagcagcg cggcgccggc ggcggccatg
     2281 atgccgagga tccaccagaa ctgcgtcggt tcgccgcccg ggaagtggac gaggggcacg
     2341 ttcatgccgt acaggccggt gagcacggtc atcggcatga agaccgccga gaagatcgtg
     2401 aggatcttca tgacctcgtt gagccggttc gacacgttcg acaggtgggc ctccaggatc
     2461 gccgcgaggc ggtcgtggaa cagcagcgct tcgtccgaga tccgcaccac gtggtcgtag
     2521 acgtcgcgga agcggtacga gacctcttcg ctgacctgcg ggaactcgcg gcggcccagc
     2581 cggccgagga tgtcgcgctg cggcaggatg atgcgccgca gagaggcgac gtcgcgcttg
     2641 aggtcgagga tctcctggtt cgtcgcgcgc gtctcggccg cctcgaagac cgagtcctcc
     2701 agctcgtcga tccgcgcctc gagcttgtcc acctccggcc ggtagtggtc caccatcgtg
     2761 tccacgatgc ggtgcatcag ggccatcggg ccttcggcca tgacgcgcgg attccggagg
     2821 cagagcgcct gcacctccgg gatcgtgcgc gaggtgccgt cggtcacggt cacgaggtag
     2881 gtcggcccga cgaagaagtc cgcgtcgtgc gtggcgaatt cgtgctgcga cgccttgaag
     2941 tcgatcccgt ggaggatgac gtaaaggtag ccatcgtagg tctcggcctt cgggtggtgc
     3001 acgacctgca gcgcgtcctc gacggccagg tcg
//
LOCUS       DAOSIF010000393         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28280_length_3026_cov_3.433861, whole genome shotgun sequence.
ACCESSION   DAOSIF010000393 DAOSIF010000000
VERSION     DAOSIF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28280_length_3026_cov_3.433861"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1280
                     /locus_tag="PLT35_11525"
     CDS             198..1280
                     /locus_tag="PLT35_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG29898.1"
                     /translation="MRDGLKIPPPGGLDLVSLGALVHRLDPGVIPFRKASSCAIHVSG
                     GEFNVAANLADCFRMRTGIATAMVDYPIGDLIAERVRAMGVTPFYKRFAHNGVNGPNM
                     ATVYSDRGHGVRAPVVFYNRANEAGGLLKPGDFDWPAIFGRGVRWFHSGGIFASLSAT
                     TGELIIEAMRAARAAGAVTSFDLNFREKLWSIWGGQRKAVDVIGRITEHVDVLVGNEE
                     DLQKGLGIPGPEVHAASRLDPSAFFAMIDSVTRKLPNVKAVATTLREVRSTNRHGWSA
                     VAWVDGQPHTAPVCELDVLDRVGGGDGFAAGFIYGLLTGETPQEAVDLGWAHGALLTT
                     FPGDTTMATLEQVRAFAKGGSARIQR"
     gene            1439..2329
                     /locus_tag="PLT35_11530"
     CDS             1439..2329
                     /locus_tag="PLT35_11530"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOG29899.1"
                     /translation="MDIDIAVVPVAGLGTRLLPATKSQPKEMLPVGRKPVVQYVVEEL
                     ARVGVKQLLFVTGPGKTSIENHFDLNPELIQTLRETGKEELLQELAFERAAVQFFYTR
                     QRQLLGLGHAVLCARSFVGRRPFIVALGDSIIGMHAESDVVRRMARCFLERDALAVVA
                     FEEVPRDEVVHYGIAAPRSAGDVFEIGDVVEKPAREEAPSTLAIAARYVFSPEIFDAL
                     ERTPPSKGGEIQLTDAIRTLIQEGGRAYGVRLKADEPRYDIGNIDAYFRAFVEFALAD
                     PRHGAAIRRHLETLLHVPHP"
     gene            2313..>3026
                     /locus_tag="PLT35_11535"
     CDS             2313..>3026
                     /locus_tag="PLT35_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29900.1"
                     /translation="MFLTRKRAYARAGLVGNPSDGYHGKTISASVRNFWAEVVLYEWD
                     SVDIVLAEGDRARFGSVQELARDVRLHGYYGGIRIIKATIKRFVEHCAEQGLALHGRN
                     FSIRYHSTIPRQVGLAGSSALVTATLRCLMDFYGVELPLDAQPTFTLKVEQEELGINA
                     GLQDRVIQAYEGLVYMDFDRARRRVVGGLPCYHYERLDPALLPPLYVAYHVSLGEPTE
                     VFHNNIRDRYNRGEALVVDA"
BASE COUNT          448 a         1082 c         1053 g          443 t
ORIGIN      
        1 catcccggtg ccggcgaccg cggcgtcgct ggcgtacttc gacgcctaca ggagcgagcg
       61 cctgccggcc aacctcaccc agggccagcg cgactacttc ggcgcgcaca cgtaccggcg
      121 cgtcgacagg cccggggtgt tccacacggc atggtgaacg acgcgccgcc gcgtcggacc
      181 agcacggagc gcgtgacatg cgtgacggac tgaagattcc tccccccggc ggcctggacc
      241 tggtgtcgct cggagcgctc gtccaccgcc tcgatccggg cgtcattccg ttccggaagg
      301 cgtcgagctg cgcgatccac gtgagcggcg gcgagttcaa cgtcgccgcc aacctcgccg
      361 actgcttccg gatgcggacc ggcatcgcga cggcgatggt ggactacccc atcggcgacc
      421 tcatcgccga gcgcgtcagg gcgatggggg tgacgccctt ctacaagcgg ttcgcgcaca
      481 acggcgtcaa cggccccaac atggcgaccg tgtacagcga ccgcgggcat ggcgtgcggg
      541 cgcccgtggt gttctacaac cgcgccaacg aggccggggg gctgctgaag ccgggcgact
      601 tcgactggcc cgccatcttc ggccgcggcg tccggtggtt ccacagcggc ggcatcttcg
      661 cctcgctctc cgctacgacc ggcgagctca tcatcgaagc catgcgggcc gcccgggccg
      721 ctggcgccgt cacctcgttc gatctcaact tccgcgagaa gctctggagc atctggggcg
      781 ggcagcgcaa ggcggtggac gtgattggcc gcatcaccga gcacgtggac gtgctcgtcg
      841 gcaacgagga ggatctccag aaggggctcg gcatccccgg ccctgaggtc cacgcggcgt
      901 ccaggctgga tccgagcgcg ttcttcgcga tgatcgacag cgtcacgaga aagctgccga
      961 acgtgaaggc ggtcgcgacc acgctgcggg aggtgcgctc cacgaaccgc cacggctgga
     1021 gcgccgtggc gtgggtggac ggccagcccc atacggcccc cgtctgcgag ctggacgtgc
     1081 tggaccgcgt cggcggcggc gacgggttcg ccgccgggtt catctacggg ctgctgacgg
     1141 gcgagacgcc gcaggaggcg gtcgacctcg gctgggccca cggcgcgctg ctcaccacct
     1201 ttcccggcga caccaccatg gcgacgctcg agcaggtgcg ggcgttcgcg aagggcggct
     1261 cggcgaggat ccagaggtag ggcgcggccc gggacgccgc gggttcgcct cgcccatggc
     1321 cgtcatcgcc gcggcgtcct tcgaggcgcc ggcggcgcgc gcacgccggc cgtccggact
     1381 tgatagaatg cccggcgcgc ccggatccga agcgggtccc gccgcgcctg gcctgatcat
     1441 ggacatcgat attgccgtcg ttcccgtcgc cggcctcggc acgcggctgc tgcccgccac
     1501 gaagtcgcag ccgaaggaga tgctgcccgt cggccgcaag ccggtcgtgc agtacgtggt
     1561 cgaggagctc gcccgcgtcg gcgtgaagca gctgctcttc gtcaccggcc cgggcaagac
     1621 ctccatcgag aaccacttcg atctgaaccc cgagctcatc cagacgctgc gcgagaccgg
     1681 gaaggaggag ctgctgcagg agctggcatt cgagcgcgcg gccgtgcagt tcttctacac
     1741 ccggcagcgc cagctgctgg gcctcggcca cgccgtgctg tgcgcgaggt cgttcgtcgg
     1801 ccgccggccg ttcatcgtgg cgctgggcga ctcgatcatc gggatgcacg ccgagagcga
     1861 cgtggtgcgg cgcatggcgc gctgcttcct cgagcgcgac gccctggccg tggtggcctt
     1921 cgaggaggtg ccgcgcgacg aggtcgtcca ctacggcatc gccgcgccgc gctccgcggg
     1981 cgacgtcttc gagatcggcg atgtcgtcga gaagcccgcg cgggaggagg ccccgagcac
     2041 actcgccatc gcggcgcgct acgtgttctc acccgagatc ttcgacgcgc tcgagcggac
     2101 gccgccgagc aagggcggcg aaatccagct gaccgacgcg atccgcaccc tcatccagga
     2161 gggcgggcgc gcgtacggcg tgcggctgaa ggcggacgag ccccgctacg acatcggcaa
     2221 catcgacgct tacttccggg ccttcgtgga gttcgcgctc gctgatccgc gccacggcgc
     2281 cgcgatccgg cgccacctgg agacgctgct ccatgttcct cacccgtaag cgcgcctacg
     2341 cccgcgccgg cctcgtcggc aacccctcgg acgggtacca cggcaagacg atctcggcga
     2401 gcgtccgcaa cttctgggcc gaggtcgtcc tctacgagtg ggactcggtg gacatcgtcc
     2461 tggcggaggg cgaccgcgcg cgcttcggct cggtgcagga gctggcgcgc gacgtacgcc
     2521 tgcacggcta ctacggcggc atccgcatca tcaaggccac catcaagcgc ttcgtcgagc
     2581 actgcgcgga gcaggggctg gcgctccacg gccggaactt ctccatccgc taccactcca
     2641 ccatcccgcg ccaggtaggc ctggccgggt cgagcgctct cgtcaccgcc acgctgcggt
     2701 gcctgatgga cttctacggc gtcgaactcc cgctggacgc gcagccgacc ttcacgctga
     2761 aggtcgagca ggaggagctc gggatcaacg ccggcctgca ggatcgcgtg atccaggcgt
     2821 acgaggggct cgtgtacatg gatttcgatc gggcacgccg gcgggtggtc ggcggcctgc
     2881 cctgctatca ctacgagagg ctcgacccgg cgctcctgcc gcccctctac gtcgcgtacc
     2941 acgtcagcct cggcgagccg acggaggtct tccacaacaa catccgcgac cgctacaacc
     3001 gcggggaggc gctggtggtc gacgcc
//
LOCUS       DAOSIF010000394         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28390_length_3017_cov_3.551992, whole genome shotgun sequence.
ACCESSION   DAOSIF010000394 DAOSIF010000000
VERSION     DAOSIF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28390_length_3017_cov_3.551992"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..860)
                     /locus_tag="PLT35_11540"
     CDS             complement(129..860)
                     /locus_tag="PLT35_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG29901.1"
                     /translation="MALDFDRVVAEEVSRSFGRRRALSRVSMVCEAGRIVGLLGPNGA
                     GKSTLLAVLSTLLPPSSGRVRYGSHEARTGGPAVRARIGLLGHDLYLYPDLTARENLA
                     FFARLYGLPDAGSRVAEALRTAGLESRGDDVVSGFSRGMRQRLALERALLHGPRLLLL
                     DEPFTGLDEASGAALVARLRGLKSDGAIVVLATHDLDLAERVLDEALVLGDGRVRATA
                     PSGGQARERYREALRATAPSGGSAS"
     gene            complement(872..2149)
                     /locus_tag="PLT35_11545"
     CDS             complement(872..2149)
                     /locus_tag="PLT35_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29902.1"
                     /translation="MTTRAARCGIGLAACLAAALGHAAAQGMDARQMSGIPIPSPDVA
                     PGTVSVRLVRGTMADNIAGHEVQLIVGAGTRSARTDAAGRALFEGVEAGVQVRAVALV
                     GEERLESSAFGMPASAGVRLVLVAGGGAASAGAEPPPGSAPSAVGTPPTADPAPMAGG
                     SGARASGLSFGSGSRIHIELEDDVLEVFYLFELVNAGTAPVSPSPELSFTLPDGAEQA
                     APIEDTQQVTIRGRRVTINNPIKPGRTPVKLAYAVRPGAGAHVVAQRFPLDWDGLRVV
                     LAGAGAARMTSPQFTSSRQGEDGGSVFVAGSGGALAAGQELAISLDGLPHRSRTGRNV
                     ALALGLVILAAGAYSAFSAGPVSGGDAGRRAQLAERRERLLADLVRVEEARRAGSISE
                     SRHAARREDLVRQLERIYGELDAQESRTAAAAG"
     gene            complement(2146..2811)
                     /locus_tag="PLT35_11550"
     CDS             complement(2146..2811)
                     /locus_tag="PLT35_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG29903.1"
                     /translation="MARTPSEKTRRGAAVPPTPPRPAPRTEPVGEPGGLRPWHLLGLA
                     TLVAVAAGVVVTRGTPVANTVTMAVLVVTAAFVAAAAARTIVPLLAPDAGERVEMIGG
                     RTRAALEREKSLVLRSIKDAEFDRAMKKISDADFQEMSVRLRSRAAGILRQLDGEGRG
                     YRALIERDLAARLGPPRAPDVPVPAQAEAASVDRACPVCGLRADPDARFCKSCGAKIE
                     EAP"
BASE COUNT          414 a         1168 c         1068 g          367 t
ORIGIN      
        1 gagggcgaag gcgaacacca acacgcacga ggccgcgaag aagaccgtcg tgtacacgat
       61 ctcgcggctg cgcatctcga cggcggcgtc cttgcgcagg atggcccacg ccacccgcac
      121 gaactccctc acgacgcgct cccgccggag ggcgccgtcg cgcgcagcgc ctcgcgatag
      181 cgttctcgcg cctgtccgcc ggagggcgcc gtcgcgcgca cgcgcccgtc gccgagtacc
      241 agggcctcgt cgagcacgcg ttcggcgaga tccaagtcgt gcgtcgcgag cacgacgatg
      301 gccccgtccg acttcaggcc gcgcaggcgg gcgacgagcg cggcgcccga cgcctcgtcc
      361 agtccggtga agggctcgtc gagcagcagc agccgcgggc cgtgcaagag cgcgcgttcc
      421 agcgccagcc gctggcgcat gccccgcgag aaccccgaca cgacatcgtc tccgcgcgac
      481 tcgaggcccg ccgtccgcag cgcttccgcc accctgctgc cggcgtcggg caggccatag
      541 agcctcgcga agaaggcaag gttctcgcgg gccgtgagat cgggatacaa gtacaggtcg
      601 tggccgagga gcccgattcg cgcccgcacg gccggcccgc ccgtccgcgc ctcgtgcgag
      661 ccgtagcgca cgcggccgga cgagggcggg agcagcgtcg agagcacggc gagcagggtg
      721 gacttccccg ctccgttcgg gccgagcagg ccgacgatcc ggccggcctc gcacaccatc
      781 gataccctgc tgagggcgcg ccgccgcccg aagctgcgcg agacctcctc ggcgacgacg
      841 cgatcgaagt cgagcgccat gccgcctcgc tctagcccgc ggccgccgcg gttcgggact
      901 cctgcgcgtc cagctcgccg tagatccgtt cgagctggcg aaccagatcc tcccggcgcg
      961 cggcgtgccg cgactcggag atcgagcccg cccgccgtgc ttcttcgacc cgcacgaggt
     1021 ccgccagcag gcgctcgcgg cgttcggcca gctgcgcgcg gcggcctgcg tcgccgccgg
     1081 acacggggcc ggcggagaag gcggagtacg cgcccgccgc gagaatgacc aggcccaggg
     1141 ccagcgccac gtttcgcccg gtgcggctcc ggtgaggaag gccgtcgagc gaaatcgcca
     1201 gttcctgccc cgccgccagg gcgccgccgc tgccggcgac gaagacgctg ccgccgtcct
     1261 cgccttgccg gctgctcgtg aactgcggcg atgtcatccg ggcagctccg gcgcctgcca
     1321 ggaccacgcg caggccgtcc cagtccagcg gaaaccgctg cgcgacgaca tgtgcgcccg
     1381 cccccggtcg cactgcatac gcgagcttca ccggggtcct ccccggcttg atggggttgt
     1441 tgatcgtgac gcggcggccc cggatcgtca cctgttgcgt gtcttcgatt ggcgcggctt
     1501 gctcggcgcc gtcgggaagc gtgaaggaga gctccggcga ggggctgacg ggcgcggtcc
     1561 cggcattgac gagttcaaag agatagaaga cctccagcac gtcgtcttca agctcgatgt
     1621 ggatgcggct gccgctgccg aaagagaggc ccgaggcgcg ggccccggag ccgccggcca
     1681 tcggggccgg gtcggccgtg ggcggcgtcc cgacggccga gggcgcgctg cccggcggcg
     1741 gctccgcgcc tgcagaggct gccccgcctc cggccaccag cacgaggcga acacccgcgc
     1801 tcgccggcat gccgaaggcg ctcgactcga gccgttcttc gccgacgagg gcaacggcac
     1861 gcacctggac gccagcctcg acgccctcga acagcgcccg gccagcggcg tcagtccggg
     1921 ccgaccgggt gccggcaccg acgatcagct ggacctcgtg gccggcgata ttgtctgcca
     1981 tcgtgccgcg caccaggcga accgacaccg tgcccggcgc cacgtccggg ctcgggatcg
     2041 ggatgcccga catctgcctc gcgtccatgc cctgcgccgc cgcgtgtccg agcgccgccg
     2101 cgaggcacgc cgccagcccg atcccgcaac gcgccgcgcg cgtggtcatg gcgcctcctc
     2161 gatcttcgcc ccgcatgact tgcagaacct ggcgtccgga tcggcccgca ggccgcagac
     2221 cgggcaggct ctgtccaccg acgcggcttc cgcctgcgcg ggcacaggca cgtccggcgc
     2281 gcgcggcggt ccgagccgcg cggcgagatc gcgctcgatg agcgcacggt acccgcggcc
     2341 ctcgccgtcc agctgccgca ggatgcccgc cgcccggctg cgcagtcgga ccgacatctc
     2401 ctggaagtcc gcgtcggaga tcttcttcat cgcccggtcg aactcggcgt ccttgatgga
     2461 gcgcagcacg agcgacttct cccgctcgag cgcggcccgc gtccgtccgc cgatcatctc
     2521 gacccgctcc ccggcgtccg gagcgaggag cggcacgatc gtccgcgccg cggcagccgc
     2581 aacgaacgcg gcggttacga ccagcacggc catcgtcacc gtgttcgcga ccggcgtgcc
     2641 gcgcgtcacc acgacgcccg ccgcgaccgc gacgagcgtg gcgaggccga ggaggtgcca
     2701 gggcctgagg ccgcccggct cgccgaccgg ctccgtgcgg ggcgcgggcc gcggcggcgt
     2761 cggcggcacg gccgcgccgc ggcgggtctt ctcactcggt gtacgcgcca tggacgcact
     2821 ccctgccgcg cgggctcccg gggggccgcg cggcgcccgc gccggccggt cagcgctccg
     2881 ctgccggcac caccgccgcg gctgcctgct cccgctcggg aagcagcgca atgaccgtgc
     2941 cgaggaacag gatgccgaat ccagtccaga tccagttgac cagcgggttg acgaccacct
     3001 ggaggctcac cgactgg
//
LOCUS       DAOSIF010000395         3009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28486_length_3009_cov_4.579553, whole genome shotgun sequence.
ACCESSION   DAOSIF010000395 DAOSIF010000000
VERSION     DAOSIF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28486_length_3009_cov_4.579553"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..2867)
                     /locus_tag="PLT35_11555"
     CDS             complement(24..2867)
                     /locus_tag="PLT35_11555"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOG29904.1"
                     /translation="MDRWQQFSGINEGYVLELYERYLTDPASVDAATREIFARWNPAE
                     REAAPPASDALAEPASARPASVSHAKAVGAVGLAESIRRYGHLAAHVDPLGAEPPGDP
                     SLHPEAHGVTRQDLDALPASLSPIAPGDGLATMADVVNALRRVYCSTTGYDYAHVFVP
                     EERRWLRHAAETGRFRAPADPIDPVALLERLTEVEVFERFLHRTFPGKTRFSIEGLDM
                     LVPILDEVIGDAAEAGVRSVFIGMAHRGRLNVMAHVLHRPVPQILTEFMDPVSPAEFH
                     EDMAWTGDVTYHAGAHRAIKHGREIQVVVSMPPNPSHLEAVDPVVEGMSRAAGTDGSQ
                     AGAPRVDASRSLPILIHGDAAFAGQGVVAETLNLGRLAGYSTGGTIHIIVNNQVGFTA
                     TARESYSTSYASGLARGFKIPIVHVNADDPEACVEAARLAIAYRTAFHRDFLIDLVGY
                     RRRGHNEGDEPAFTQPLLYDAIASHPTVRERWARTLVERGTVAAGAPDALVAKHMDRL
                     QRAMDALEPERNLAEAPPPAPPGAASRASTSVPLDALAGLNAALLAGPAGFTAHRKLA
                     RLWEKRAQIFADPDARSIDWAAAETLAFASILADGTPIRLTGEDAARGTFSHRHAVLH
                     DARTGRSDVPLQRLPQARAAFDVHNSPLSEYAAVGFEFGYNLQAPSHLVLWEGQYGDF
                     INGAQIAVDEYVVSARGKWGSRPSLVLLLPHGHEGAGPDHSSARPERFLQLAAGINMR
                     VVNCTTAAQYFHLLRRQAALLLEDPLPLVVLTPKSLLRHPMVASSPRELAEGQFRPLI
                     NDDEAKARAGGIRRVVLCTGKVYVDLAGSDRRAAAPHVALCRLEQLYPIAPQDLRAML
                     DGYPAAEEIVWVQEEPANMGYWDFVGPRLTCAAGGRPVRLVARPRSASPAEGSAARHG
                     RMQRILVEAAFAPVKPADCAEAGGAPAAARTAG"
BASE COUNT          398 a         1067 c         1131 g          413 t
ORIGIN      
        1 gcggccatgg ctgtccgatc ctctcagccg gcggtccgtg cagcagccgg cgcgccgccg
       61 gcttccgcgc aatccgccgg tttgacgggg gcaaacgcgg cctccaccag gatccgctgc
      121 atgcgcccgt ggcgcgccgc cgatccttcc gccgggctgg cgctgcgcgg ccgggcaacc
      181 agtcgcaccg ggcgcccgcc ggcggcgcac gtgaggcgcg gcccgacgaa gtcccagtac
      241 cccatgttgg ccggctcctc ctggacccag acgatctcct cggcggcagg atagccgtcg
      301 agcatcgcgc gcagatcctg cggcgcgatc gggtacagct gttcgaggcg gcagagcgcg
      361 acgtgcggcg ccgccgcgcg ccggtcgctt ccggcaaggt cgacatatac cttgcccgtg
      421 cacagcacga cgcgccggat cccgccggca cgcgccttcg cctcgtcgtc gttgatcaga
      481 ggacggaact gcccctcggc cagctcgcgc gggctcgacg ccaccatggg gtgtctcaac
      541 aggctcttgg gggtcagcac gacgagcggc agcgggtcct ccaggaggag cgcggcctgc
      601 cggcggagca ggtggaagta ctgcgccgcc gtggtgcaat tgaccacccg catgttgatg
      661 cccgccgcca gttgcaggaa gcgctccggc cgcgcgctcg agtggtccgg gccggcgcct
      721 tcgtggccgt gaggcagcag cagcacgagc gacgggcggg atccccactt gcctcgggcc
      781 gaaacgacgt actcgtcgac ggcgatctgg gcgccgttga tgaagtcgcc gtactggcct
      841 tcccacagca ccaggtgcga cggcgcctgc aggttgtagc cgaactcgaa gccgacggcg
      901 gcgtattccg acagggggga gttgtggacg tcgaacgccg cgcgcgcctg cggcagccgc
      961 tgcaggggca cgtcgctgcg gcccgtgcgg gcgtcgtgga gcacggcgtg gcggtggctg
     1021 aacgtgcccc gcgcggcgtc ttcgccggtc agccggatcg gggtgccgtc ggcgaggatc
     1081 gatgcgaacg ccagcgtctc ggcggcggcc cagtcgatcg acctggcgtc gggatcggcg
     1141 aagatctgcg cccgcttctc ccacaggcgc gccagcttgc ggtgcgccgt gaacccggcc
     1201 ggcccggcga gcagcgcggc gttgaggccg gcgagcgcgt cgaggggcac cgacgtcgag
     1261 gcgcgagagg cggcgccggg cggcgccggc ggcggtgcct ccgcgaggtt ccgctccggt
     1321 tccagcgcgt ccatggcccg ctgcagccgg tccatgtgct tcgccaccag cgcgtcgggc
     1381 gcgcccgccg cgacggttcc gcgctcgacg agcgtgcggg cccaccgctc gcgcacggtg
     1441 gggtgcgacg cgatggcgtc gtacaacagc ggctgcgtga acgccggctc gtcaccctcg
     1501 ttgtggccgc ggcgccggta ccccacgaga tcgatgagga agtcgcggtg gaaggccgtg
     1561 cggtaggcga tggcgaggcg cgccgcctcc acgcacgcct ccgggtcgtc ggcgttgacg
     1621 tgcacgatgg ggatcttgaa gccgcgcgcc agcccgctgg cgtacgacgt gctgtacgac
     1681 tcgcgggcgg tggcggtgaa cccgacctgg ttgttgacga tgatgtgaat cgtgccgccg
     1741 gtgctgtacc cggccaggcg gccgaggttc agcgtctcgg ccaccacgcc ctggccggcg
     1801 aacgccgcgt cgccgtggat gaggatcggc agcgaccggc tcgcgtccac gcgcggcgcg
     1861 ccggcctgcg acccgtcggt gcccgcggcg cgcgacatcc cctcgacgac gggatcgacc
     1921 gcctcgaggt gactcgggtt ggggggcatc gacaccacca cctggatctc gcgcccgtgc
     1981 ttgatggccc ggtgcgcgcc ggcgtggtag gtgacgtcgc cggtccacgc catgtcctcg
     2041 tggaactccg ccggcgacac cggatccatg aactcggtga ggatctgcgg gaccggccgg
     2101 tgcagcacgt gcgccatcac gttgaggcgg ccgcggtgcg ccatgccgat gaacacggag
     2161 cggacgccgg cctcggcggc atcgccgatc acctcgtcga ggatcggcac gagcatgtcg
     2221 agcccttcga tcgagaaccg ggtcttgccg ggaaacgtgc ggtgcaggaa gcgctcgaag
     2281 acctcgacct cggtcagccg ctcgaggagc gcgaccggat cgatcgggtc ggcgggcgcg
     2341 cggaaccgcc cggtttcggc ggcgtggcgc agccaccggc gctcttcggg cacgaacacg
     2401 tgcgcgtagt cgtacccggt ggtcgagcag tagacgcgcc gcagcgcgtt gacgacgtcg
     2461 gccatcgtcg ccaggccgtc gccgggcgcg atcgggctga gcgacgccgg cagcgcatcg
     2521 agatcctgcc gggtgacgcc gtgcgcctcg ggatgcaggg acgggtcgcc gggcggctcg
     2581 gcgccgagcg gatcgacgtg cgcggccagg tgcccgtagc gccgaatcga ctcggcgaga
     2641 ccgaccgccc cgaccgcctt ggcgtgcgag accgacgcgg gccgagctga ggcaggctcg
     2701 gccaaagcgt cggaggccgg aggcgcggcc tcgcgctcgg ccggattcca ccgcgcgaat
     2761 atctcccggg tcgcggcgtc caccgaggcc ggatcggtca ggtagcgctc gtagagttcg
     2821 aggacgtacc cctcgttgat ccccgagaac tgctgccagc ggtccatggc agccccatcg
     2881 tagccgatcc cgccggaaat cccgccgggg gccgggacgc gagcctcggc gccgctagct
     2941 ctcgtccggc ttcggcgtct tcgccgtgct cggcggcagc gccggcggcg cgcgtcgtct
     3001 cgacggtcg
//
LOCUS       DAOSIF010000396         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28533_length_3005_cov_4.745763, whole genome shotgun sequence.
ACCESSION   DAOSIF010000396 DAOSIF010000000
VERSION     DAOSIF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28533_length_3005_cov_4.745763"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PLT35_11560"
     CDS             complement(<1..974)
                     /locus_tag="PLT35_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOG29905.1"
                     /translation="MLSRPRTPTLVLIFVTCAMALGSAAASPQQRRGAPAPAAPAVQP
                     PPPPAPALGRLPAILTLREQAEMYNAWLTVRLERLLPELMRAEGFDMWLVICREGHED
                     PVFSSLVPFNTMYASRTTMLVFTDKGPEGIERLSVSRSGIGAFYRSVWDPDRIGQWER
                     LAEIVTERSPKKIGINESDTFNFGDGITAALKKQLLAALPQEYHARIQPAERLAIRWL
                     ERRTPDEMEVYPHIVAIAHGIIAEAFSRTVITPGVTTTSDVQWYFRERARQLGLETWF
                     HPTVDIQRPRDTPYGQSDVIHRGDLLHCDFGIRYLKLCTDTQRMAYVL"
     gene            1115..1480
                     /locus_tag="PLT35_11565"
     CDS             1115..1480
                     /locus_tag="PLT35_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOG29906.1"
                     /translation="MTGYGNAGRRGMPEWRRLGTAFAALAAVTAVCGAWLGLRNPIVA
                     GLSHLLVVPLAAAASTLRVAVATSVLAVLALNCFFLPPVGTFRLDDPENRIALFVLFV
                     VGYVGSRISVMARERAALS"
     gene            1739..>3005
                     /locus_tag="PLT35_11570"
     CDS             1739..>3005
                     /locus_tag="PLT35_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOG29907.1"
                     /translation="MVATTVLLSFISFWRAAAIVLADLGSSAFYAGGIAEQAVGRAAP
                     WFILGVMLFSWAVRAVYIESSVMFVRGGVYRVVKEAMGGTMAKLSVSALLFDYVLTGP
                     ISAVSAGLYIAALAADVLARFGHRLPLASNSAAALVAVCILLYFWRRNTQGLRESSDD
                     ALRIMQVTTVMVVTLLVWAAFTLLTRGGQPPVAPTPEHLRFSDAALGWLKGTPFPAIT
                     GVALLIGFGHSLLAMSGEESLAQVYREIESPKAANLRRAGMVVFLYSLIFTASVSFLA
                     VALIPDAVRPQYFNNLISGIAMHLAGPPPLRLLFQSFVVVVGFLILAGAVNTAIVGSN
                     AVLSRVSEDGVLPAWFRRPHARFGTTYRFLNVIVGLQALTVVLSGGHIYLLGEAYAFG
                     VIWSFALKSLAVLVLRFKEPGRREWRVPVN"
BASE COUNT          356 a         1056 c         1087 g          506 t
ORIGIN      
        1 ctgagcacgt aggccatccg ctgcgtgtcg gtgcagagct tcaggtaccg gatgccgaag
       61 tcgcagtgga gcaggtctcc gcggtggatg acgtcgctct gcccgtacgg cgtgtcccgc
      121 ggccgctgga tgtccaccgt cgggtggaac cacgtctcga ggccgagctg gcgcgcccgc
      181 tcgcggaagt accactgcac gtcggaggtc gtcgtcacgc cgggcgtgat gaccgtacgg
      241 gagaacgcct cggcgatgat gccgtgggcg atcgccacga tgtgggggta cacctccatc
      301 tcgtcgggcg tccgccgctc gagccagcgg atggcgaggc gctcggccgg ctggatccgc
      361 gcgtggtact cctgcggcag ggcggcgagc agctgcttct tcagcgcggc cgtgatgccg
      421 tcgccgaagt tgaaggtgtc cgactcgttg atgccgatct tcttcgggct gcgctcggtg
      481 acgatctccg caaggcgctc ccattggccg atccggtccg ggtcccatac gctgcggtag
      541 aacgcgccga tcccggagcg gctgacgctc aggcgctcga tgccctcggg ccccttgtcg
      601 gtgaagacca gcatggtcgt gcggctcgcg tacatcgtgt tgaacggcac gagcgaggag
      661 aacacgggat cctcgtggcc ctcgcggcag atgacgagcc acatgtcgaa gccctcggcc
      721 cgcatcagct cgggcaggag gcgctcgagg cgcaccgtga gccaggcgtt gtacatctcg
      781 gcctgctcgc ggagcgtgag gatggccggc aggcggccaa gcgccggcgc agggggcggc
      841 ggcggctgga ccgccggggc ggccggggcc ggcgcgccgc ggcgctgctg cggcgaggcc
      901 gccgccgatc cgagcgccat ggcgcaggtg acgaagatca acacgagcgt gggcgtgcgc
      961 ggccgtgaca gcattccggt tcctcttggg gcgggttcgg ccgcccgccg gcgcgggcgg
     1021 atcgtcgtcc ggacccggta cgccggccag tctacaggat cgctgcgaag atcgcagcgg
     1081 gcggccggca cgggcagccg gcgcccctgc tccgatgacc ggctacggca acgccggccg
     1141 gcgcgggatg ccggagtggc ggcgtcttgg aaccgcgttc gcggccctcg ccgccgtcac
     1201 cgccgtctgc ggagcctggc tcggcttgcg caacccgatc gtcgccggcc tgtcccatct
     1261 cctggtcgtg ccgcttgcag cggccgcgtc cacgctgcgc gtggcggtgg ccacttcggt
     1321 gctcgccgtg ctggcgctca actgcttctt cctcccgccg gtcggcacgt tcaggctcga
     1381 cgatcccgag aaccggattg ccctgttcgt gttgttcgtc gttgggtacg tcggcagcag
     1441 gatctccgtc atggcccgcg agcgcgcggc gctgtcctga tcgatcccgg cctgcccgac
     1501 atggacggca tcgcggtatg cgcgagatcc gcgcgcgctg gaccgtcccg atcctcatgc
     1561 tgtcggcccg cggcgccgag cgcgagaaag cggcggcgct cgacgaaggc ggcagccgca
     1621 cgccggcggc gggtttagaa tcgaggcgat ggccagcgcc gccgcgagcc cctcggatgc
     1681 cgggggccgg actccccccg gcgggccggc ggccccgccc tccggtccga ctcgggtcgt
     1741 ggtcgccacg acggttctgc tgtcgttcat ctccttctgg cgcgcggccg ccatcgtgct
     1801 ggccgacctc gggtcgtcgg cgttctacgc cggcggcatc gcggagcagg ccgtcggccg
     1861 ggccgcgccc tggttcatcc tcggcgtgat gctgttctcg tgggccgtcc gggcggtcta
     1921 catcgagagc tccgtcatgt tcgtgcgcgg cggggtgtac cgcgtcgtga aggaggcgat
     1981 gggcgggacg atggccaagc tgtcggtctc ggccctgctg ttcgactacg tcctcaccgg
     2041 gcccatcagc gccgtgtccg ccggcctcta cattgcggcc ctggccgcgg acgtgctcgc
     2101 gcggttcggg caccgcctgc cgctcgcgtc caatagcgct gcggccctcg tggccgtctg
     2161 catcctgctg tacttctggc gccgcaacac ccagggcctg cgggaatcga gcgacgacgc
     2221 cctgcgcatc atgcaggtca cgaccgtgat ggtcgtgacg ctgctcgtgt gggccgcctt
     2281 cacgctgctc acgcgaggcg ggcagccgcc cgtggcgccg acgccggagc acctccggtt
     2341 ctcggacgcc gcgctgggct ggctgaaggg gacgccgttt cccgccatca ccggggtcgc
     2401 gctcctgatc ggcttcggcc actcgctgct cgcgatgagc ggcgaggagt ccctggcgca
     2461 ggtctaccgc gagatcgaat cgccgaaggc cgcgaacctg cgccgcgcgg gcatggtcgt
     2521 cttcctctac agcctgatct tcaccgcgtc ggtgagcttc ctggccgtgg cgctcatccc
     2581 ggacgcggtg cggccgcagt acttcaacaa cctgatctcg ggcatcgcca tgcacctggc
     2641 gggcccgccg cctctccgcc tgctgttcca gtcgttcgtc gtcgtcgtcg ggttcctgat
     2701 cctggcgggc gcggtgaaca cggccatcgt cggctcgaac gccgtgctga gccgcgtgtc
     2761 cgaggacggc gtgctgcccg cctggttccg gcggccgcac gcgcggttcg ggacaaccta
     2821 ccgcttcctg aacgtgatcg tggggctcca ggcgctcacg gtcgtgctca gcggggggca
     2881 catctacctg ctcggcgagg cgtatgcctt cggcgtcatc tggagcttcg cgctcaagtc
     2941 gctcgccgtg ctcgtgctcc gcttcaagga gcccgggcgc cgggagtggc gcgtgccggt
     3001 gaacg
//
LOCUS       DAOSIF010000397         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28535_length_3005_cov_3.923729, whole genome shotgun sequence.
ACCESSION   DAOSIF010000397 DAOSIF010000000
VERSION     DAOSIF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28535_length_3005_cov_3.923729"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PLT35_11575"
     CDS             <1..1146
                     /locus_tag="PLT35_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG29908.1"
                     /translation="RLGMDPVELRLKNVPAASQTRNGIPYSTTGLADCLREGATAFGW
                     AEARGRRPAPSHIKRGVGVAAGMWQGGAGGPPSTAIVKLFPDGSANLNMGASDIGCGT
                     KTWGAQIVAEELHVGLDRISVEHADTGTTQFATPSGGSKTVPTESPAIRSAALDVKRQ
                     LLSMAAEQLKVPAADLDLRGGEIVSKADPAKKLGVGQVQAFGRRGVLVGVGYRGPNPQ
                     GKATNPFGAQFAEVEVDTKTGEIRLLRFLSAHDSGRVLNEKMFRNQVNGGVTMGIGFG
                     LTEERVMDRQLGKLLTANLHDYKMPTALDAPADKTVIAIDPHDTFCNTGAKGLGEPAT
                     IPTAPAIANAVFHATGLRVTDAPMSPARVLRAMAALKPAPAGRKGEE"
     gene            1146..2123
                     /locus_tag="PLT35_11580"
     CDS             1146..2123
                     /locus_tag="PLT35_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOG29909.1"
                     /translation="MLRTFAYVRPGSVGEALEQLEERGARVHAGGTDLLGCLRDGVFE
                     ASKVVSLSRIGELRGITAAPDGELRIGALATIAALAAHPAVKERFTALADAASAVASP
                     QLRNQGTLGGNLCQRPRCWYYRGGYDCVRKGGETCYAIGGENQYHAIFGGGACWIVHP
                     SDTAPALMALDARVRLVGPKGARVLPLEKFYLLPDDDATKETVLEPGEIVLGVQVPPP
                     AKGTRSAYRKVRARGSWDFAMAGLAWAIAMDDGRVADARLVLSGVAPAPWRLHAVEKL
                     LIRQRLDAKTVARAAAAAVVGAKPLEQNAYKVPLVRGLVEDALGAASRA"
     gene            2161..2967
                     /locus_tag="PLT35_11585"
     CDS             2161..2967
                     /locus_tag="PLT35_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOG29910.1"
                     /translation="MSRRVGPLLAGGVAACALALLGAPAFAAFGQHGALRVEVRAAAS
                     KAPVAGARVEVAGAPEAAVTDVSGRARLTNLQAGPASVRVSADAFRPVEATVVVGEAD
                     ATLEVFLVPLELRVDESIIVAAAREERRGFDVPRSTVVVSRQALDERAARTAPEILQD
                     RSGGWVQKTNHGGGSPFIRGLVGNQVLVLVDGVRLNNATFRLGPNQYMNTIDPFGLDR
                     VEIVRGSGSVVYGSDALGGVVNLVTPAPELQGSGVRARGAVATRLAGLGM"
BASE COUNT          422 a         1051 c         1137 g          395 t
ORIGIN      
        1 cgcctgggga tggacccggt cgagctccgc ctcaagaacg tcccggccgc gagccagacg
       61 cgaaacggga tcccgtactc cacgaccggg ctcgccgact gcctgcggga gggcgcgacg
      121 gcattcgggt gggccgaggc ccgcggccgg cgccccgctc cgtcgcacat caagcgcggc
      181 gtcggcgtgg cggccggcat gtggcaaggc ggcgccggcg ggcctccgtc caccgccatc
      241 gtcaagctgt ttcccgacgg cagcgccaac ctcaacatgg gcgcgagcga catcgggtgc
      301 ggcaccaaga cgtggggcgc gcagatcgtg gccgaggagc tccacgtcgg gctggatcgg
      361 atttccgtcg agcacgcgga caccggcacg acgcagttcg ccacgccgag cggcggcagc
      421 aagaccgtgc cgaccgaatc gccggcgatc cgctcggccg ccctcgacgt gaagcggcag
      481 ctgctgtcga tggccgccga gcagctgaag gtgccggccg ccgacctgga cctgcgcgga
      541 ggcgagatcg tctcgaaggc cgacccggcc aagaagctgg gtgtcggcca ggtccaggcg
      601 ttcgggcgcc gcggcgtcct cgtgggcgtc ggctaccggg ggcccaatcc ccaggggaag
      661 gccacgaatc ccttcggcgc gcagttcgcc gaagtcgagg tcgacacgaa aacgggtgag
      721 atcaggctgc tgcgcttcct gtccgcccac gacagcggcc gggtcctcaa cgagaagatg
      781 ttccggaacc aggtgaacgg cggcgtcacc atgggcatcg gcttcggcct caccgaggag
      841 cgcgtcatgg accgccagct cggcaagctg ctgacggcca acctgcacga ctacaagatg
      901 ccgacggcgc tcgacgcgcc ggcggacaag accgtcatcg ccattgatcc ccatgacacc
      961 ttctgcaaca ccggcgcgaa ggggctcggt gagccggcga cgattccaac ggcgcccgcg
     1021 attgccaacg ccgtgttcca cgcgaccggc ctccgcgtga cggacgcgcc gatgagcccc
     1081 gcccgcgtgt tgcgcgccat ggcggcgctg aagccggccc cggccggccg gaagggggaa
     1141 gagtgatgct gcggacgttt gcctacgttc gtccgggctc cgttggcgag gcgctcgaac
     1201 agctggagga gcggggagcg cgggtgcacg ccggcgggac cgacctcctc ggctgcctgc
     1261 gcgacggggt cttcgaggcc agcaaggtag tgagcctctc ccggattggc gagctgcgcg
     1321 gcatcaccgc cgcgccggac ggggagctgc ggatcggggc gctggcgacc atcgcggcgc
     1381 tggcggctca cccggccgtc aaggagagat tcacggcgct ggcggacgcc gcgtcggccg
     1441 tcgccagccc gcagctgcgc aaccagggca ctctcggcgg caacctgtgc cagcgtccgc
     1501 ggtgctggta ctaccgcggc ggctacgact gcgtccgcaa gggcggagag acgtgctacg
     1561 ccatcggcgg agagaaccag taccacgcca tcttcggcgg cggcgcgtgc tggatcgtcc
     1621 acccgtcgga cacggcgccc gccctgatgg ccctcgacgc cagggtgcgg ctggtcggcc
     1681 cgaagggcgc gcgcgtcctc ccgctcgaga agttctacct gctgcccgac gacgatgcga
     1741 cgaaggagac cgtcctcgag ccgggcgaga tcgtgctcgg cgtgcaggtg ccgcctccgg
     1801 cgaaggggac ccggagcgca taccggaagg tccgggcgcg cgggtcgtgg gacttcgcca
     1861 tggcgggcct tgcctgggcg attgcgatgg acgacggcag ggtggccgac gcgcggctcg
     1921 tcttgtccgg cgtcgcgccc gccccgtggc gcctgcacgc ggtggagaag ctgctgatcc
     1981 ggcagcggct cgatgcgaag acggtggcca gggcggcggc ggcggcggtg gtcggggcga
     2041 agccgctcga gcagaacgcc tacaaagtgc cgctcgtgcg cgggctcgtt gaggacgcgc
     2101 tcggcgcggc ttcccgggcc tagcgccgcg cggcgcctgc gctctgaagg acggttgccg
     2161 atgagtcgtc gagtgggccc gctcctggcg ggcggggtcg cggcgtgcgc gctggcgctg
     2221 ctcggggcgc cggcgtttgc cgccttcggt cagcatggcg ctctccgcgt cgaagtgcgg
     2281 gcggcggcct cgaaagcccc cgtcgccggg gcgcgcgtcg aggtcgccgg cgcgcctgag
     2341 gccgccgtca ccgacgtgtc cggccgcgcc cgcctgacga acctccaggc cggcccggcg
     2401 tcggtgcgcg tgagcgccga cgccttcagg cctgtcgaag cgacggtcgt ggtcggcgag
     2461 gcggacgcca cgctcgaggt cttcctggtg cctctcgagc tccgcgtgga cgagtcgatc
     2521 atcgtcgccg cggcccgaga ggaacgccgc gggttcgacg tgcctcgatc caccgtcgtc
     2581 gtgtcgcggc aggccctcga cgagcgcgcg gcccgcacgg cgcccgagat cctgcaggat
     2641 cgcagcggcg gctgggtcca gaagacgaac cacggcggcg ggtcgccctt catccgcggc
     2701 ctggtcggca accaggtgct cgtgctggtg gacggcgtca ggctgaacaa cgcgacgttc
     2761 cgcctggggc ccaaccagta catgaacacg atcgatccgt tcggcctcga ccgcgtcgag
     2821 atcgtcaggg ggtcgggctc ggtcgtgtac ggcagcgacg cgctgggcgg cgtcgtcaac
     2881 ctggtcacgc ccgcgcccga gctgcagggc tcaggcgtgc gcgcgcgcgg cgccgtcgca
     2941 acgcgactgg caggcctggg catgtagcag acggtgcgcg ccgagggctc ggtggccggc
     3001 acgcg
//
LOCUS       DAOSIF010000398         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28610_length_2999_cov_3.912704, whole genome shotgun sequence.
ACCESSION   DAOSIF010000398 DAOSIF010000000
VERSION     DAOSIF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28610_length_2999_cov_3.912704"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /gene="purD"
                     /locus_tag="PLT35_11590"
     CDS             <1..1084
                     /gene="purD"
                     /locus_tag="PLT35_11590"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG29911.1"
                     /translation="LTVVGPEGPLTAGLVDSLGAAGHRACGPTRLAAEIESSKAFAKR
                     LMAACGVPTARSVTCTALSDALGVLRDERFGYPVVVKADGLAAGKGVTVALDRASAET
                     AVGEMMVDRRFGDAGSAVVIEEYLRGREASFFVLTDGVRARVLPSAEDHKRAFDGDGG
                     PNTGGMGAFAASPLVTEAMADRILDSIVEPALRGLRGEGRPYRGFLYCGLMITDDGPK
                     VVEFNARLGDPEAQVVLPALDEDLLPHLWNAAGGAIEPGAFRVRPDRYVGVVLASGGY
                     PGAFETGKAIAGLDDPALRDGAQVFYAGVADRGGALVTAGGRVLTVVAGGAGFAEARE
                     AAYAAASRISFEGMHYRRDIGVRAIG"
     gene            1193..1690
                     /gene="purE"
                     /locus_tag="PLT35_11595"
     CDS             1193..1690
                     /gene="purE"
                     /locus_tag="PLT35_11595"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOG29912.1"
                     /translation="MPPIQVLILMGSDSDAPIMKGAADVLAELGVSCEMTVASAHRSP
                     ARVQRLLEEAPGRGVRVFIVGAGAAAHLAGMVAAHTAKPVIGVPIDSSPLHGFDALLS
                     TVQMPPGVPVATVAVGKAGATNAGVLAAQMLALADPALAARLDAYKQRLAEKVEQAAA
                     RMGGR"
     gene            1723..>2999
                     /locus_tag="PLT35_11600"
     CDS             1723..>2999
                     /locus_tag="PLT35_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG29913.1"
                     /translation="MSRCRVPSPEPESESRVKFALRTFGCRANQADSFEIEESLRAAG
                     GVPAGVDDADVVVVNTCSVTGAADRDARNLVRRISARNPAARIFVTGCYATRAPGAVA
                     GLPGVVRVVANDEKGGIASVALGAAARGGRPGSAPSGAGLGAPPGAPPGAPVPAESGG
                     PGADPTSGLGPGVMGRTAFPLRVQTGCDEACAFCIVPSTRGRSRSRPVADVLADAARL
                     AEAGYKELWVVGVHLGAYGRDLPSSPSLLDLLRALDRAPADLTFRLSSLEPMDCPAGI
                     VDLVAASGRFAPHFHLPLQHADDRVLGAMRRPYTFAACRELVDYIHKALPDAAIGADL
                     VAGFPGESAAEADACAAAVAGLPLTHLHVFPYSDRPGTVAAGMTPKVAPAEVRRRAER
                     LRAIGTALAGRFAQENVGAVRKGLTLDDGATVLT"
BASE COUNT          370 a         1060 c         1135 g          434 t
ORIGIN      
        1 cctgaccgtc gtcggcccgg aagggccctt gacggccggc ctcgtcgact ccctcggcgc
       61 cgccggccac cgggcgtgcg gcccgacccg gctggctgca gagatcgaat ccagcaaggc
      121 gtttgccaag cgcctgatgg ccgcctgcgg cgtgccgacc gccaggtcgg tcacctgcac
      181 ggcgctctcc gacgccctcg gcgtgctgcg cgacgagcgc ttcgggtatc ccgtcgtcgt
      241 caaggccgac ggcctcgccg ccggcaaggg ggtcaccgtc gccctcgacc gcgcgtcggc
      301 cgagaccgcc gtcggcgaga tgatggtgga ccggcgcttc ggcgacgccg ggtcggccgt
      361 cgtcatcgag gagtacctgc gggggcgcga ggcgtccttt ttcgtgctga ccgacggcgt
      421 ccgggccagg gtgcttccct cggccgaaga ccacaagcgg gcgttcgacg gcgatggcgg
      481 cccgaacacg gggggcatgg gggccttcgc cgcgagcccc ctcgtcaccg aagcgatggc
      541 ggaccgcatc ctggactcga ttgtcgagcc ggccctgcgc ggcctccggg gcgaggggcg
      601 cccgtacagg ggcttcctct attgcggcct catgatcacg gacgacggcc cgaaggtggt
      661 cgagttcaac gcgaggctcg gagaccccga ggcgcaggtc gtgctgccgg cgctcgacga
      721 ggacctgctg ccgcatctct ggaatgcggc cggcggggcg atcgagccag gcgccttccg
      781 cgtgcggccg gaccgctacg tcggcgtcgt cctggcgtcg ggcggctacc ccggcgcgtt
      841 cgagacgggc aaggccatcg ccgggctgga tgatcccgcg ttgagggacg gggcccaggt
      901 gttctacgcg ggcgtcgcgg atcgcggcgg cgcgctcgtg acggcgggcg gccgcgtgct
      961 gacggtcgtg gccggcgggg ccgggttcgc cgaagcgcgt gaggcagcct atgcggccgc
     1021 ctcgagaatc tcgttcgagg ggatgcacta tcggcgcgac attggcgtga gggcgattgg
     1081 ataggattca gcatccagga ttcggacctc agaaacgaat gcccggaacc ccgaattccg
     1141 agtcccgagc cccgagtctc gagtcccgag ttccgagtaa tgggggtacg cgatgccgcc
     1201 gatccaagtc ctgatcctga tggggtccga ctccgacgcg ccgattatga aaggcgccgc
     1261 cgacgtgctg gccgagctgg gcgtctcgtg cgaaatgacc gtggcctcgg cgcaccgctc
     1321 gcccgcgcgc gttcagcgcc tgctcgagga ggcgccgggc cgcggcgtgc gcgtgttcat
     1381 cgtcggggcc ggagccgccg cgcacctggc cgggatggtg gccgcgcaca cggcgaagcc
     1441 cgtgattggc gtgcccatcg actcgtcgcc gctccacggc ttcgacgcgc tcctgtcgac
     1501 ggtgcagatg ccgcccggcg tgccggtggc gacggtggcg gtggggaagg cgggcgccac
     1561 gaacgcgggc gtgctggccg cgcagatgct ggcgctcgcg gacccggccc tggcggcgcg
     1621 gctcgacgcg tacaagcagc gccttgccga gaaggtggag caggcggcgg cgaggatggg
     1681 ggggaggtag aagccaggat tctgaattca gaatggggta cggtgtcccg ctgtcgagtc
     1741 ccgagtcccg agcccgagtc cgagtcccgt gtgaagttcg ccctccgcac cttcggctgc
     1801 cgcgcgaacc aggccgactc gttcgagatc gaagaatccc tgcgcgccgc cggcggcgtg
     1861 ccggccggcg ttgacgatgc cgacgtggtc gtcgtcaaca cgtgcagcgt gaccggcgcc
     1921 gcggatcggg acgcgcgaaa cctcgtccgt cggatctcgg cgcgcaatcc ggctgcgcgc
     1981 atcttcgtca ccggctgcta cgccacacga gcgcctggcg ctgtcgccgg cctgccgggg
     2041 gtcgtgcggg tcgtggcgaa cgacgagaag ggcgggattg ccagcgtggc gctgggcgcg
     2101 gccgcccgcg ggggccggcc cggttccgcg ccctcgggcg cggggctcgg cgcgccgccc
     2161 ggcgctccgc ccggcgcccc ggttcccgcc gagagcgggg gcccgggcgc cgatcccaca
     2221 tcgggcctcg ggcccggtgt gatgggccga accgccttcc ctctgcgcgt gcagacggga
     2281 tgcgacgagg cgtgcgcctt ctgcatcgtg ccctcgacgc gggggcgctc gcgcagccgg
     2341 cccgtcgccg acgtgctggc cgatgccgcg cggctcgcgg aggccggcta caaggagctg
     2401 tgggttgtcg gcgtgcatct cggggcgtac ggccgcgatc tcccgagctc gccgtcgctg
     2461 ctggacctgc tgcgcgcgct cgatcgcgcc cccgcggacc tgacgttccg cctcagctcc
     2521 ctggagccga tggactgtcc tgccgggatc gtggacctcg tcgccgcctc gggccgcttc
     2581 gcgccgcact ttcacctgcc gcttcagcac gccgacgaca gggtgctcgg cgcgatgcgc
     2641 cggccgtaca cgttcgcggc ctgccgcgag ctggtcgact acatccacaa ggcgctgccg
     2701 gatgccgcga tcggcgccga tctcgtggcc gggtttccgg gcgaatccgc ggccgaggcc
     2761 gacgcgtgcg ccgccgcggt cgccggcctg cccctcacgc acctgcacgt atttccctac
     2821 tccgacaggc cgggcaccgt tgccgccggg atgacgccga aggtggcgcc ggcggaggtg
     2881 cgccggcggg cggaacggct gcgcgcgatt gggaccgcgc ttgccggccg cttcgcgcag
     2941 gaaaacgtcg gcgcagtcag gaagggcctc acgctcgacg atggcgcgac cgtgctgac
//
LOCUS       DAOSIF010000399         2996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28653_length_2996_cov_4.326080, whole genome shotgun sequence.
ACCESSION   DAOSIF010000399 DAOSIF010000000
VERSION     DAOSIF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28653_length_2996_cov_4.326080"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLT35_11605"
     CDS             <1..591
                     /locus_tag="PLT35_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG29914.1"
                     /translation="AGAPLDRQFAQVAASLPADAPADFVLTYRPSALQAPSIAPTRVA
                     DSRGRLLVGEVDDDNAWAMGGVAGHAGLFGTAGAVGAFARAVLRALAGDARAERQIAS
                     AATLRAFLAPSGVPGSSRSLGWDLMRPSSSCGSRMPASAFGHTGFTGTSLWIDPAGDF
                     YAVLLSNRVYPKAGPGDAMQALRRAFHDAVAAAIGE"
     gene            complement(578..1267)
                     /locus_tag="PLT35_11610"
     CDS             complement(578..1267)
                     /locus_tag="PLT35_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG29915.1"
                     /translation="MVALLVAIAVSGVLISVAMPVWRTAIQREREEELVFRGRQYARA
                     IQLYQRKYANAYPPTIEVLVEQRFLRKQYKDPVNKNADFEILYQSTLAQRMATQSGRR
                     GTPGPTQGRAVQGGASEGGLVTQGSAFGSQVAGPQGGVVGVASKSTAASIRALEGRTK
                     YNEWQFVWIQPAAAGRAGRRGQPVPGQRGFQRGSQGETEGAFQGGFQGGRQGGPQRGR
                     GAGPGGPIIPR"
     gene            complement(1318..2547)
                     /locus_tag="PLT35_11615"
     CDS             complement(1318..2547)
                     /locus_tag="PLT35_11615"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOG29916.1"
                     /translation="MTARMGRRASRPDGRAWGFAAVLLAALAASCDKMPLVAPSDTTI
                     TLYASTTIVGLNGTVDITATVIEPAGTPVQNGTVVTFTTTLGTLSPAEARTSGGKATV
                     RLTAGSTSGTAEIHAFSGSSRASGLSISIGAAAAGSVTLLANPSALPAAGGTVLLTAV
                     VNDKAGNRLAGVPVAFSTDNGVLSQTAVMSDGNGEARTSLTTTATSSVKASVVGGSSG
                     EVSASVSVKVRVGPTVTVSIPSSSLVPGVPAVFSVSVTPGGAAVQRAIIDFGDGASQA
                     VSNAGLSSVTHTYHSSGTYVVKATATDAAGETASATASVSVQTVVVTVSLSVGASVIT
                     TTAPAEFTATATTTPIGSAIERFEWDFGDGSTRVTSGGTTSHLYQSGGGRRYTVTVRA
                     VTLGGASGSAQREIVVQ"
     gene            complement(2570..>2996)
                     /locus_tag="PLT35_11620"
     CDS             complement(2570..>2996)
                     /locus_tag="PLT35_11620"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG29917.1"
                     /translation="VTGTDICTGRRRRDGRRGFTLIELVVVVSLIVILAGIGMAQYSN
                     AVKRAQEAALKEDLFRMRDVIDQYYADKQRYPDTLEALVTDGYLRQLPKDPFTGSAET
                     WVPVETEPDPANPSAEIGIQDVKSGSDKTALDGSKYSEW"
BASE COUNT          377 a         1115 c         1038 g          466 t
ORIGIN      
        1 gccggcgcgc cgctcgatcg gcagttcgcg caggtcgcgg cttcgctgcc agccgacgcg
       61 ccggcggact tcgtcctgac ctaccgcccg tccgcgctcc aggcaccctc catcgccccg
      121 acccgcgttg ccgactctcg cggccggctg ctcgtcggcg aggtcgacga cgacaacgcg
      181 tgggcgatgg gcggcgtggc cggccacgcc gggctgttcg gcacggccgg cgccgtcggc
      241 gcgttcgccc gagcggtcct gcgggcgctt gccggcgacg cgcgcgcgga acggcagatc
      301 gccagcgccg cgacgctgcg cgcgttcctg gcgccgtccg gcgtgcccgg cagctctcgc
      361 agtctgggat gggacctcat gcggccgtcg tcgtcgtgcg gatcgaggat gccggcgtcg
      421 gcgttcggcc acaccggctt caccgggacg tcgctctgga tcgacccggc cggcgacttc
      481 tatgcggtgc tgctgtcgaa ccgcgtgtac ccgaaggcgg gacctggcga cgcgatgcag
      541 gccctgaggc gcgcctttca cgacgccgtg gcggctgcta tcggggaatg atcgggccgc
      601 cgggccctgc gcccctgcct cgctggggcc cgccctgccg ccctccctgg aacccgccct
      661 ggaaggcccc ctcggtttcg ccctgggagc cgcgctggaa gccccgctgc cctggcacgg
      721 gctggccccg ccggccggcc cggcccgctg cggccggctg gatccagacg aactgccatt
      781 cgttgtactt cgtgcgcccc tcgagcgctc gaatcgaagc cgccgtgctc ttgctcgcca
      841 cgccgacgac gccgccttgc ggcccggcca cctggctgcc gaaggccgag ccctgcgtca
      901 cgaggccgcc ttccgaggcg ccgccctgga cggcccggcc ctgggttggg ccgggcgtgc
      961 ctcgccgccc cgactgcgtc gccatgcgct gcgcgagcgt ggactggtag aggatctcga
     1021 agtccgcgtt cttgttgacc gggtccttgt actgcttgcg caggaagcgc tgctcgacga
     1081 ggacctcgat tgtcggcggg tacgcgttgg cgtatttgcg ctggtagagc tggattgccc
     1141 gggcgtactg ccggccgcga aagacgagtt cctcttcgcg ctcgcgctgg atcgccgtgc
     1201 gccacaccgg catggcgacg ctgatcagca cgcccgacac ggcgatggcg acgagcaacg
     1261 ccaccatcgc atatcctgct tcccttccgc gcgccggacg gcccatctcc cccgccgcta
     1321 ctgaacgacg atctcccgct gggcggagcc ggacgcgccg ccgagggtga cggcgcggac
     1381 cgtcacggtg taccgcctgc cgccgccgga ctggtacagg tggctcgtcg tgccgcccga
     1441 tgtgacgcgc gtggagccgt cgccgaagtc ccattcgaag cgctcgatgg cggatccgat
     1501 cggggtggtg gtcgccgtgg cggtgaactc ggctggcgca gtggtagtga tcaccgacgc
     1561 gcccacgctc agggacaccg tgacgacgac ggtctgcacg ctcaccgacg cagtcgccga
     1621 agcggtctcc ccggcggcgt cggtggccgt ggccttcacc acataggtcc cgctcgagtg
     1681 gtacgtgtgc gtgaccgagc tcaggcccgc gttcgacacc gcctggctgg cgccgtcgcc
     1741 gaagtcgatg atcgcgcgct ggacggccgc gccgcccggc gtgacgctca cgctgaacac
     1801 ggcggggacg cccgggacca ggctgctcga cgggatcgac accgtcaccg tcggcccgac
     1861 gcgaaccttg acgctcaccg acgcggacac ctctccgctg gatccgccga cgacagaggc
     1921 cttgaccgag gaggtcgcgg tcgtcgtcag gctggtgcgg gcttcgccgt tgccgtcgct
     1981 catgaccgcc gtttgcgaca gcacgccgtt gtcggtcgag aaggcgaccg ggacgccggc
     2041 gaggcggttc ccggccttgt cgttcacgac cgccgtcagc agcaccgtgc cgcccgccgc
     2101 cggcagcgcg gacgggttgg ccagcagggt cacgctcccg gcagcggccg cgccgatgga
     2161 gatggacagc cccgaggcgc gcgacgagcc cgagaacgcg tgaatctccg ccgttcccga
     2221 cgtcgaccct gccgtcaggc gcacggtggc cttgccgccg gaggtccgtg cctcggccgg
     2281 cgacagcgtg ccgagcgtcg tcgtgaaggt cacgacggtg ccgttctgca ccggcgtgcc
     2341 ggcgggctcg atcaccgtgg cggtgatgtc caccgtgccg ttcaggccga cgatcgtggt
     2401 gctggcgtac agcgtaatcg tggtgtccga cggcgccacc agcggcatct tgtcgcacga
     2461 cgccgccagc gccgccagaa ggaccgcggc gaagccccat gcgcgcccgt ccgggcgcga
     2521 ggcccgccgt cccattcgtg ctgtcatggc gccctgactc cctgatccgt caccactccg
     2581 agtacttgct gccgtcgagg gccgtcttgt ccgacccgct cttcacgtcc tgaatgccga
     2641 tctccgccga cgggttcgca ggatcgggct cggtctccac cggcacccac gtctcggccg
     2701 agccggtgaa cgggtccttc ggaagctgcc gcaggtagcc gtccgtcacc agcgcctcga
     2761 gcgtgtcggg atagcgctgc ttgtcggcgt agtactgatc gatcacatcg cgcatcctga
     2821 agaggtcctc cttgagggcg gcctcctgcg cgcgcttcac ggcgttgctg tattgagcca
     2881 tgccgatgcc cgccaggatc acgatgagcg acacgaccac caccagctcg atcagcgtga
     2941 acccccgccg cccgtcgcgg cgcctgcggc ccgtgcagat gtcagtccca gtcacg
//
LOCUS       DAOSIF010000400         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28710_length_2992_cov_3.190671, whole genome shotgun sequence.
ACCESSION   DAOSIF010000400 DAOSIF010000000
VERSION     DAOSIF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28710_length_2992_cov_3.190671"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PLT35_11625"
     CDS             complement(<1..1287)
                     /locus_tag="PLT35_11625"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOG29918.1"
                     /translation="MRETPGPPTSLRLSAAAEPAAWRRRPFRDNPLQVLLVIFALVAV
                     LAGMMTLLYRSSRLAPDFLSEFVLYSLSIVDLTMLVGLVFFLARSVIKMLVERKRGLP
                     FARYRAKLVLALLGMTIIPAVLVLFAGSQLIFNSVDRWFNAPMDEILESSQRIASDYY
                     SDRQRQVSGETARLARALGAMDLASADLRPIRDALSEPVTAQRLTLVEVFRAVPGLDG
                     TPQAVPVVDVAAASAGAARASSGRLAARAAGGEPDSWAREPLENGGELLRAAAVVRKG
                     DGTAAGVVVGSVELTGDMALRARRMTKAYESYSQLRALKRPLTGVYLSFFLMVTLMIL
                     VSATWMGLYLAKRITRPVGLLAAAAREIGAGHFDHRVERETADEFGSLVDAFNSMADE
                     LATNRRRLEQSSIDLERKHAEVEERRRFTEAVLDRVA"
     gene            complement(1305..1838)
                     /locus_tag="PLT35_11630"
     CDS             complement(1305..1838)
                     /locus_tag="PLT35_11630"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOG29919.1"
                     /translation="MMRVRLVALVLGAFVLEAGGQRGVDVGAVARDGRVYVSAKLTGD
                     VLSGLEDAIQAGLTATITYEAELRHPVSVWFDSTIASARVAASVQHDTLTRRYQISRT
                     VDGRVDDSRISGDRADVARFVTGFDRLPLFSTADLDPNEEYYVRVRVRTRPSTTWFFW
                     PWGRDAASGVARFTFIP"
     gene            complement(1835..2293)
                     /gene="nrdR"
                     /locus_tag="PLT35_11635"
     CDS             complement(1835..2293)
                     /gene="nrdR"
                     /locus_tag="PLT35_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOG29920.1"
                     /translation="MKCPYCGHMGDKVVDSRESKEGDVIRRRRECLECGRRFTSYERV
                     DDIPYMVIKKDGRRERFERQKLIAGMLRACEKRPVTVSALEAIADRIESILQDRPEKE
                     IGTGEIGVFVMQDLKRLDKVAYVRFASVYRDFRDIGEFMVELKDLISAKE"
     gene            complement(2290..>2992)
                     /locus_tag="PLT35_11640"
     CDS             complement(2290..>2992)
                     /locus_tag="PLT35_11640"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOG29921.1"
                     /translation="AASAFATIILERLLDLLTVLLLFGFYLLCFHPAAAEQHAAMLRA
                     LKSGGLLTASGAIAGLAGMMIAAGYPETLAAWAQKVERVVPPRAASLVARLVKTFLQG
                     LAIVRQPGRLAVAGLLSFPLWLSIAAGIWLGARAFAIDMGYPASFLLMALLVVGVTVP
                     TPGSVGGYHKAYQIGAVAFFGVANDRAVAAALVAHAISIVPVTLAGVVFLAQEGISLG
                     RLRTLAAPDPGEEAR"
BASE COUNT          466 a         1121 c         1013 g          392 t
ORIGIN      
        1 cgccacccgg tcgagcacgg cctcggtgaa gcgccggcgc tcctcgacct ctgcgtgctt
       61 gcgctcgagg tcgatgctcg actgctcgag gcgccgccgg ttcgtggcca gctcgtccgc
      121 catgctgttg aacgcgtcca cgagcgaccc gaactcgtcc gcggtctccc gctcgacgcg
      181 gtgatcgaag tgcccggctc cgatctcgcg cgcggcggcg gccagcaggc cgaccgggcg
      241 cgtgatgcgc ttggccaggt acagccccat ccacgtggcg ctcacgagga tcatcagggt
      301 gaccatcagg aagaacgaca ggtacacgcc cgtcagcggg cgcttgaggg cccggagctg
      361 gctgtacgac tcgtaggcct tcgtcatgcg ccgggcgcgg agcgccatgt cgcccgtcag
      421 ctccaccgac ccgacgacca cgccggcggc ggtaccatcg cccttccgca cgactgcggc
      481 ggcgcggagc agttccccgc cgttctccag gggctcgcgc gcccagctgt ccggctcgcc
      541 cccggccgcc cgcgccgcca gccggcccga cgaagcccgc gccgcccccg ccgaagccgc
      601 cgccacgtcc acgaccggga cggcctgggg cgtgccgtcg agcccgggca cggcgcggaa
      661 gacctcgacg agcgtcagcc gctgggccgt caccggctcg gacagcgcgt cgcggatcgg
      721 ccggagatcg gccgacgcca ggtccatcgc cccgagggcg cgggccaggc gcgccgtttc
      781 gccgctcacc tgccgctgcc ggtccgagta gtagtcgctc gcgatccgct gagacgactc
      841 gaggatctcg tccatcggcg cgttgaacca gcggtccacg ctgttgaaga tcagctggct
      901 gcccgcgaag agcacgagga ccgccgggat gatcgtcatg ccgagcagcg cgagcaccag
      961 cttggcccgg tagcgcgcga agggcaggcc tcgcttccgc tcgacgagca tcttgatcac
     1021 gctgcgggcg aggaagaaga cgagccccac cagcatggtg aggtccacga tggacaggga
     1081 gtagaggacg aactcgctca ggaagtccgg cgccagccgg ctcgaccggt agagcagcgt
     1141 catcatgccc gccagcaccg cgacgagggc gaagatgacg agcaggacct gcagcgggtt
     1201 gtcgcggaac ggacgccggc gccacgcggc cggctcggcg gccgccgaca ggcgaagcga
     1261 ggtgggcggt cccggcgtct cgcgcatgat cggcctctcg ggccctacgg gatgaaggtg
     1321 aagcgcgcga cgccgctcgc ggcgtcgcgc ccccacggcc agaagaacca cgtcgtgctg
     1381 ggccgcgtcc gcacgcgcac ccgcacgtag tactcctcgt tcggatccag gtcggccgtg
     1441 ctgaagagcg gcaggcggtc gaaccccgtc acgaaccgcg ccacgtcggc gcggtccccg
     1501 gagatccgcg agtcgtcgac gcgcccgtcg acggttcggc tgatctggta gcgccgcgtc
     1561 agcgtgtcgt gctgcaccga ggcggccacc cgcgccgagg cgattgtgct gtcgaaccag
     1621 acgctcaccg ggtgcctcag ctcggcctcg tacgtgatcg tcgccgtgag ccccgcctgg
     1681 atggcgtcct cgaggccgct gagcacgtcg cccgtcagct tcgcggagac gtacacccga
     1741 ccgtcccgcg cgaccgcgcc gacgtccacg ccgcgctggc ctcccgcctc cagcacgaac
     1801 gcgccgagca ccagggccac caggcggacg cgcatcattc cttcgcgctg atgagatcct
     1861 tgagctccac catgaactcg ccgatgtccc ggaagtcccg gtacacggag gcgaaccgga
     1921 cgtacgccac cttgtcgagg cgcttcaggt cctgcatgac gaagacgccg atctcaccgg
     1981 tgccaatctc cttctcgggg cggtcctgca ggatgctctc gatgcggtcg gcgatcgcct
     2041 cgagcgccga cacggtcacg ggccgtttct cgcaggcgcg cagcatgccg gcgatgagct
     2101 tctgccgctc gaaccgctcg cgccgtccgt ccttcttgat caccatgtac gggatgtcgt
     2161 ccacgcgctc gtagctcgtg aaccggcggc cgcactcgag gcactcccgc cgccggcgga
     2221 tcacgtcgcc ctccttgctc tcgcgcgagt ccacgacctt gtcgcccatg tggccgcagt
     2281 acgggcactt catcgtgcct cctccccggg gtccggcgcc gccagcgtcc tgagccggcc
     2341 gaggctgatc ccctcctgcg cgaggaagac cacgcccgcc agcgtcaccg gcacaatcga
     2401 gatcgcgtgc gcgacaagcg ccgcggccac cgcacgatcg ttcgccaccc cgaagaacgc
     2461 gactgccccg atctggtagg ccttgtggta ccccccgacc gaccctggcg tcggcacggt
     2521 cacgccgacg acgagcagcg ccatcaggag gaacgacgcc gggtagccca tgtcgatcgc
     2581 gaacgcccgg gcgcccagcc agatccccgc ggctatcgac agccacaggg ggaaggagag
     2641 caggccggcc accgccagcc ggcccggctg ccggacgatg gcgaggcctt gcaggaacgt
     2701 cttgacgagc cgcgcgacga gcgaggcggc gcgcggcggc accacccgct cgaccttctg
     2761 cgcccacgcc gccagcgtct cggggtagcc ggccgcgatc atcatgccgg ccagccccgc
     2821 aatggccccg ctcgcggtca ggaggccgcc cgacttcagg gcgcgcaaca tggccgcgtg
     2881 ctgctcggcc gccgccggat ggaagcacag caggtagaac ccgaagagca gcaggaccgt
     2941 gagaaggtcg agcaagcgct cgaggatgat cgtcgcaaag gcggacgcgg ca
//
LOCUS       DAOSIF010000401         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_28738_length_2990_cov_3.122998, whole genome shotgun sequence.
ACCESSION   DAOSIF010000401 DAOSIF010000000
VERSION     DAOSIF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28738_length_2990_cov_3.122998"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLT35_11645"
     CDS             complement(<1..413)
                     /locus_tag="PLT35_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29922.1"
                     /translation="MSRKAAERARCALCGAKDVSEPRGDERYCPDCWDKKIAVEDIVA
                     KEFVLKRYIRAHSAEKYLVYHSTLKRPCGQLIVIDDGYDLFLTLTLYPTFVWDDAAYH
                     LESDPESRSFAEILVDVVVTEVIEPWGGGKWHLEV"
     gene            473..871
                     /locus_tag="PLT35_11650"
     CDS             473..871
                     /locus_tag="PLT35_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG29923.1"
                     /translation="MERPAHLGLRHLALNVRDVGMMRAFYVDVLGFRVEWEPDPDNVY
                     LTSGRDNLALHRAEGNAEAARPGALDHLGLIVGSAGLVDAWAAYLDERGVPLDTRPKT
                     HRDGARSCYFRDPEGNRVQILHHPPISPLG"
     gene            complement(953..>2990)
                     /gene="glgP"
                     /locus_tag="PLT35_11655"
     CDS             complement(953..>2990)
                     /gene="glgP"
                     /locus_tag="PLT35_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOG29924.1"
                     /translation="VRRRWFGGTGLPARRRRRLPVRRAGQHHGNFRWRVSRCFRDLRA
                     ARASRETWWALTHGEAAAKTIAYFSAEFALHQSLPIYAGGLGVLAGDFCKEASDLGMP
                     FVGVGFMYPQGYFHQRVSAEGWQEETYEHLDWDDAPVRPAVTPAGERCLVEVPLGTSA
                     VKALVWHVKVGKVSLLLLDTGLEENAPWDRELSARLYGGNQETRIQQEIVLGLGGVRA
                     LRAMGIEPAVWHLNEGHAAFVTLQRIWDHLEQGSTFDAALEQVRATTVFTTHTPVPAG
                     HDAFPFQLVETHLAGAWGSSLGAFRDRLMALGSYDNGGGTQFNMTALALRTAAGINAV
                     SQPHGEVTRRMWTSIWPGTPAEKLPIKAITNGVHASTWVAAPLGKLFDDSFGTEWRDR
                     LDEPEFWDRVLDIPDEALWRVRLSMKAELFRSIQDRARKRWTHDKVSAAQVVAAGTLL
                     DQSALTIGFARRFAGYKRPDLIFHDAARLASILSTARRPVQIIFAGKAHPADDQGKHH
                     LQQVYRRAADPAFGGRIAFVDDYDLHLAQYLVHGCDVWLNHPRKPMEASGTSGMKAAI
                     NGIPHLSIGDGWWAEGFNGNNGWLIQGKAHGTDQEAVDAADAEALYALLEEQVIPTFY
                     DRDAHDVPRAWLRVVKEAIRSTMPAYTTRRMMKQYAEELYLPAIMSTMRVGLGD"
BASE COUNT          432 a         1050 c         1023 g          485 t
ORIGIN      
        1 tgcacctcca ggtgccactt gcccccgccc cagggctcga tgacctcggt gacgacgaca
       61 tcgaccagga tttccgcgaa gctccgcgac tcgggatcgc tctccaggtg gtaagcggcg
      121 tcgtcccaga cgaaggtggg gtagagggtg agcgtcagga acagatcgta gccgtcgtcg
      181 atgacgatga gctgcccgca ggggcgcttc agcgtggagt ggtagaccag gtacttctcc
      241 gcgctgtggg cgcggatgta gcgcttcagc acgaactcct tcgcgacgat gtcctcgacg
      301 gcgatcttct tgtcccagca gtcggggcag tagcgctcgt caccacgggg ctcggacacg
      361 tccttcgccc cgcagagcgc gcaccgcgcc cgctcggcgg ccttcctcga catgcgcgcc
      421 atcttatcac tgcctcttgt cccgcgctcc ccgcgcctac tacgatctcc ccatggagcg
      481 tcccgcccac ctcggcctgc gccacctcgc cctcaacgtc cgcgacgtgg gaatgatgcg
      541 ggcgttctac gtcgacgtgc tcggcttccg ggtcgagtgg gaaccggatc cggacaatgt
      601 ctacctgact tcgggccgcg acaacctggc cctgcaccgt gcggagggga acgcggaggc
      661 ggcccgcccc ggggccctgg atcacctcgg gttgatcgtc gggtcggccg gcctggtgga
      721 cgcgtgggcg gcgtacctcg acgagagggg cgtgcctctc gacacgcggc cgaagaccca
      781 ccgagatggg gcccgttcct gctacttcag ggacccggag ggcaaccggg tgcagatcct
      841 ccatcacccc ccgatcagcc ccctcggctg aaggggccag gatcagggcc cggctgcttc
      901 gcctggaagc ggggccaggg ccggcgtgcc ggggcctggc ccggtcaggg cgtcaatccc
      961 cgagcccgac gcgcatcgtc gacatgatgg cgggcaggta tagctcctcg gcgtactgct
     1021 tcatcatccg acgggtcgtg taggcgggca tggtgctccg gatggcttcc ttgaccacgc
     1081 gaagccatgc ccgcggcaca tcgtgcgcgt cccggtcgta gaaggtgggg atgacctgct
     1141 cctccagcag cgcgtagagg gcctcggcgt ccgccgcgtc cacggcctcc tggtccgtgc
     1201 cgtgcgcctt gccctggatg agccagccgt tgttgccgtt gaacccctcg gcccaccagc
     1261 cgtcgccaat gctcaggtgg gggatgccgt tgatggctgc tttcatgccg ctcgtgccgc
     1321 ttgcctccat cggcttccgc gggtggttga gccagacgtc gcagccgtgc acgaggtact
     1381 gggcgaggtg gagatcgtag tcgtccacga aggcgatgcg gcccccgaag gccgggtcgg
     1441 cggcgcgccg gtagacctgc tgcaggtggt gcttgccctg gtcgtcggcc ggatgggcct
     1501 tgcccgcgaa gatgatctgc acgggacgcc tcgccgtcga gaggatggac gccagccggg
     1561 ccgcgtcgtg gaagatcagg tcgggccgct tgtagccggc gaaccgacgc gcgaacccga
     1621 tggtcagcgc cgactgatcc agcagcgtgc cggccgccac gacctgcgcc gcgctcactt
     1681 tgtcgtgcgt ccagcgcttc ctggcccggt cctggatgga tctgaagagc tcggccttca
     1741 tcgacagccg gaccctccag agggcctcgt ccgggatgtc gaggacgcgg tcccagaact
     1801 ccggctcgtc gagccgatcc cgccactcgg tgccgaagct gtcgtcgaag agcttgccga
     1861 gaggcgcggc gacccaggtg ctggcgtgga cgccgttggt gatcgccttg atgggcagct
     1921 tctcagcggg ggtcccgggc cagatggacg tccacatccg gcgggtgacc tcgccgtgcg
     1981 gctggctcac cgcgttgatg ccggccgcgg tccgcagcgc gagcgccgtc atgttgaact
     2041 gcgttccgcc tccgttgtcg taggagccga gcgccatgag gcggtcccgg aaggccccca
     2101 ggctgcttcc ccaggccccg gcgaggtgcg tttcgacaag ctggaacggg aacgcgtcgt
     2161 gcccggccgg cacgggcgtg tgggtcgtga acaccgttgt cgcccggacc tgctccagtg
     2221 ccgcgtcgaa ggtcgagccc tgctcgaggt gatcccagat gcgctgcagg gtcacgaagg
     2281 ccgcgtgccc ctcgttgaga tgccagaccg ccggctcgat acccatggcc cgcagcgcgc
     2341 ggacgccgcc caggcccagc acgatctcct gctggatccg ggtctcctgg ttgccgccgt
     2401 acagccgcgc cgacagctcg cggtcccacg gggcgttctc ttcgagcccg gtgtcgagca
     2461 gcagcagcga gaccttgccg accttcacgt gccacacgag cgccttgacg gcgctggtgc
     2521 cgagcggcac ctcgacgaga caccgttctc cggccggggt gacggcgggc cggaccggcg
     2581 cgtcgtccca gtccaggtgc tcgtaggtct cctcctgcca gccctccgcg gagacccgct
     2641 ggtggaagta gccctgcgga tacatgaacc cgacgccgac gaacggcatc cccaggtcgc
     2701 tcgcttcctt gcagaagtcg ccggcgagga cgccgaggcc gcccgcgtag atcggcagcg
     2761 actggtgcag cgcgaactcc gccgagaagt acgcgatggt cttggcggcc gcctcgccat
     2821 gcgtcagggc ccaccaggtt tcgcgcgagg cccgcgccgc ccgcaggtcg cggaaacaac
     2881 gcgatacccg ccaccgaaaa ttcccgtggt gctgccctgc gcgccgaact gggagacgcc
     2941 gtcttcgccg agctggaagc cctgttccgc caaaccagcg acgacgaact
//
LOCUS       DAOSIF010000402         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_29006_length_2966_cov_3.318104, whole genome shotgun sequence.
ACCESSION   DAOSIF010000402 DAOSIF010000000
VERSION     DAOSIF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29006_length_2966_cov_3.318104"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1976
                     /gene="pilB"
                     /locus_tag="PLT35_11660"
     CDS             246..1976
                     /gene="pilB"
                     /locus_tag="PLT35_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOG29925.1"
                     /translation="MALRIGELLLKERRITPEQLQEALLYQKAHGIKLGSALVKLGYV
                     RDDDITGLLSRQYGVPSINLAQFEIDPAIIKLVTPETAQKYQIVPLSRVGATLTIAMA
                     DPTNVFAMDDLKFMTGYNIEPVVAAESAVHDAIERSYGGSRSEPSALDVASKALDEMP
                     GVLDADVEVLDELDEISVDALARQGEEAPVIRLVNVLLMSAISKGASDIHIEPYEKEY
                     RIRFRIDGVLYNIMSPSLKFRDAIASRIKIMSKLDIAEKRLPQDGRIKIRYNDQGRSK
                     ELDFRVSVLPTLFGEKIVLRLLDRDKLMLDMTGLGFEPGPLERYEAAIQKPWGMVLVT
                     GPTGSGKTNTLYSSISRLNKADTNIMTAEDPVEFNLPGINQVQIRENIGLTFAASLRA
                     FLRQDPNIILVGEIRDFETAEIAVKAALTGHLVLSTLHTNDAPGTISRLMNMGIEPFL
                     VSSSVNLICAQRLVRRVCPFCRQPHPSPPAALTKIGFSPEEAHAVVPYRGAGCERCNG
                     TGYKGRVGLFEVMEVTDGIRELILVGASGLELRRRAVEEGMISLRASGLQKIRDGVTT
                     IEEVLRETVM"
     gene            2014..>2966
                     /locus_tag="PLT35_11665"
     CDS             2014..>2966
                     /locus_tag="PLT35_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOG29926.1"
                     /translation="MTTLPDLLRHTIDVNASDLHLTAGSPPQVRVNGILQRLDMPELT
                     PSETKRFAYSVLTDAQKKRFEEALELDFSFGIRGLARFRCNVFSQRGAVAAVYRVILE
                     NIRGFKELGLPEVVSTLADRPRGLVLVTGPTGSGKSTTLAALVDKINRERRQHILTIE
                     DPIEYIHPHKQCLVNQREVLSDTHGFAPALRAALREDPDVVYIGEMRDLETVESALRI
                     AETGHLTFATLHTNSAAQTINRIVDVFPSHQQTQIRTQLSMVLEGIVCQALLPRADGR
                     GRVVAVEVLIPTPAIRNLIRDDKVHQIYASMQAGQEKVGTQT"
BASE COUNT          517 a          981 c         1060 g          408 t
ORIGIN      
        1 ccgggtccgg gctcgagcct gatggcgagc gcctcccgtg ccgacaacta aggagtcgga
       61 gccgcccgcg gttccagcgg tgcggccggt tgacgcggga ttcgggcgga cgaccgagcc
      121 cggccgaggc cgcgcgggtc cgccggcccg gcccggccgg accgcgccgc gcggcggagc
      181 ccggcgacgg ttttcgcgta cggctgacaa agatcgtctc ggagaggcga cgcggttcga
      241 aacccatggc actgcgcatc ggtgagctgc tcctgaagga gcgccgcatc acgccggagc
      301 aactccagga ggccctgctc taccagaagg cccacgggat caagctgggc tcggccctgg
      361 tgaaactcgg ctacgtgcgc gacgacgaca tcacgggcct cctgagccgc cagtacgggg
      421 tgccctcgat caacctggcc cagttcgaga tcgacccggc catcatcaag ctcgtcacgc
      481 cggagacggc gcagaagtac cagatcgtcc ccctcagccg cgtgggcgcc acgctcacca
      541 tcgccatggc ggacccgacc aacgtgttcg ccatggacga tctcaagttc atgaccggct
      601 acaacatcga gccggtcgtg gcggccgagt cggcggtcca cgacgccatc gagcggtcgt
      661 acgggggaag ccggagcgag ccgagcgcgc tggacgtcgc ctcgaaggcg ctcgacgaga
      721 tgcccggggt cctcgacgcg gacgtcgagg tgctcgacga gctcgacgag atcagcgtcg
      781 acgcgctggc ccgccagggc gaggaagcgc cggtcatccg cctggtcaac gtgctgctga
      841 tgtcggcgat ctcgaagggc gccagcgaca tccacatcga gccgtacgag aaggagtacc
      901 ggatccggtt ccgcatcgac ggggtgctct acaacatcat gagcccgtcg ctgaagttcc
      961 gcgacgcgat cgcgtcccgg atcaagatca tgtccaagct ggacatcgcc gagaagcgcc
     1021 tgccgcagga cggccggatc aagatccggt acaacgacca gggccgatcc aaggagctgg
     1081 acttccgcgt gtcggtgctg ccgacgctct tcggggagaa gatcgtgctt cgcctcctcg
     1141 accgcgacaa gctgatgctg gacatgacgg ggctgggctt cgagcccggc ccgctggagc
     1201 ggtacgaggc ggcgatccag aagccctggg gcatggtgct cgtgacgggc ccgacgggga
     1261 gcggcaagac gaacacgctc tattcgtcga tctcgcgcct gaacaaggcc gacaccaaca
     1321 tcatgacggc ggaggacccg gtcgagttca acctgcccgg gatcaaccag gtccagatcc
     1381 gggagaacat cgggctgacg ttcgccgcct ccctgcgggc gttcctgcgc caggacccca
     1441 acatcatcct cgtcggcgag atccgggact tcgagacggc cgagatcgcg gtgaaggccg
     1501 cgctgacggg gcacctcgtg ctgtccacgc tgcacacgaa cgacgcgccc gggacgatca
     1561 gccggctgat gaacatgggc atcgagccct tcctggtgtc gagctcggtg aacctcatct
     1621 gcgcccagcg cctggtgcgg cgggtctgcc cgttctgcag gcagccgcac ccgtcgccgc
     1681 cggcggcgct gacgaagatc gggttcagcc cggaggaggc ccacgcggtg gtgccctacc
     1741 gcggggcggg ctgcgagcgg tgcaacggga cggggtacaa ggggcgcgtc gggctgttcg
     1801 aggtgatgga ggtgaccgac ggcatccggg aactgatcct cgtgggggcg tcggggctcg
     1861 agctgcgccg tcgggcggtc gaggaaggga tgatcagcct gcgggcgagc gggctgcaga
     1921 agatccggga cggcgtcacg acgatcgagg aagtgctgcg cgagacggtg atgtaggccc
     1981 gggaattgca cgggcgggga aggaaggacc ggcatgacca cgctcccgga tttgctccgg
     2041 cacaccatcg acgtgaacgc gtcggacctg cacctgacgg cgggctcgcc gccgcaggtg
     2101 cgggtgaacg gcatcctcca gcggctggac atgccggagc tgacgccgtc ggagacgaag
     2161 cggttcgcct acagcgtgct gaccgacgcc cagaagaagc ggttcgagga ggcgctggag
     2221 ctggacttct ccttcggcat ccggggcctg gcgcggttcc gctgcaacgt gttcagccag
     2281 cggggcgcgg tggcggcggt ctaccgcgtg atcctcgaga acatccgcgg cttcaaggag
     2341 ctcgggctgc cggaagtggt ctcgacgctg gccgaccggc cgcgcgggct cgtgctcgtg
     2401 accggcccga cgggcagcgg caagagcacc acgctcgcgg cgctggtcga caagatcaac
     2461 cgcgagcgcc gccagcacat cctgaccatc gaggatccga tcgagtacat ccacccgcac
     2521 aagcagtgcc tggtgaacca gcgcgaggtg ctcagcgaca cccacgggtt cgcgccggcc
     2581 ctgagggcgg cgctgcgcga ggacccggac gtcgtgtaca tcggcgagat gcgcgacctg
     2641 gagacggtcg agtccgcgct gcgcatcgcc gagacgggcc acctgacgtt cgcgacgctg
     2701 cacaccaact cggcggccca gacgatcaac cgcatcgtgg acgtgttccc gtcgcaccag
     2761 cagacgcaga tccggacgca gctctcgatg gtgctcgagg gcatcgtgtg ccaggcgctg
     2821 ctgccgcggg cggacggccg ggggcgcgtc gtggcggtcg aggtcctgat cccgacgccc
     2881 gccatccgga acctcatccg cgacgacaag gtgcaccaga tctacgcgtc gatgcaggca
     2941 gggcaggaga aggtcgggac gcagac
//
LOCUS       DAOSIF010000403         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_29017_length_2965_cov_4.041237, whole genome shotgun sequence.
ACCESSION   DAOSIF010000403 DAOSIF010000000
VERSION     DAOSIF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29017_length_2965_cov_4.041237"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLT35_11670"
     CDS             <1..279
                     /locus_tag="PLT35_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29927.1"
                     /translation="DNERARVDAFGHDLARTRYRWALDDEAWAWFRDAYLAAGGSSDA
                     VAHQRFWFAVALCVSALFRARSSHSGVSRPLDRLRQLALETHAGGGHR"
     gene            276..1508
                     /locus_tag="PLT35_11675"
     CDS             276..1508
                     /locus_tag="PLT35_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG29928.1"
                     /translation="MTDSGGGVVVFCTQYKGHQKRMMPIVAGLIGAGIPVHVFADQGA
                     RADMEGVGAGFSDLYWRQPYTEIDPGTTRPTSGWSVTFAGLYGEDVAREVEPLRPRLI
                     LHDTLMPVARVVAIRLGLPRVVMRMGQAFDPQKLRQRYATDPSMAPSVSDKCREAVRI
                     LQEKHGIAEASPFLAFSDAGGELNIYSEPSQFMAARPGEPPDTVEYFGSLWPASHSAA
                     DGAPSPFGPLSEGRVRVYISFGTVVWDVHASEAQAALEALADAIAEMPAVAALISLGG
                     GDLPALAARLARRNVRVERYVDQIRVLRDASVFVTHHGMNSTHEAIYYGVPMISYPFI
                     WDQPGIAARCQELGLAVPLAPEPLARVSVPDVRRALDRIASGGDELRRRLAEARQWEL
                     DVIARRPAVIERIKALMA"
     gene            1757..2965
                     /locus_tag="PLT35_11680"
     CDS             1757..2965
                     /locus_tag="PLT35_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOG29929.1"
                     /translation="MNKRVYIAAYHQSEFGKLMGMSVPEIVKASVAGVCGEIEADPSI
                     LDVGSVGATCTYLLNQQGLLAGLLAAVPGLAGKPIEAVENACASGGQAILSVIYKLLV
                     GQGEAGIAVGFEKMRDAEGKMDGRLIGRTLGVFSHPDEREGKVHIFPHIFAEVMADYM
                     TRHGVTERDLAHVSATEYANANSNPYAQMRKIKVTLDDCANLGDRNRYIVEGLPLKTY
                     DCSQITDGYAAVIVATEEGLKRLGVAKADAVEIAGYAQATDSLLRADRDVLRAEGAFT
                     AMSKAYAMAGLTPADVNVAEIHDCFTVMGAIGTEVIGKAPFGRGAQYWVDGRARADGE
                     CGINTSGGLIAKGHPVGATGVAMVGWSAWQLLGRVPPALQVTGAKAAATFNIGGPICA
                     SVCTVLKPGA"
BASE COUNT          428 a         1030 c         1081 g          426 t
ORIGIN      
        1 gacaacgaac gggcccgcgt tgacgccttc ggacacgacc tggccaggac gcggtaccgc
       61 tgggcgctcg acgacgaggc ctgggcctgg ttccgcgacg cctacctcgc ggccggcgga
      121 agcagcgacg ccgtggcgca ccagcggttc tggttcgcgg tggccctctg cgtgagcgcg
      181 ctgttccgcg ctcgctcgtc gcattccggc gtgtcccggc cgctggaccg tcttcggcaa
      241 ctggccctgg aaacccatgc gggaggagga catcgatgac cgattcaggt gggggcgtgg
      301 tggtcttctg cacgcagtac aaaggccacc agaaacgcat gatgcccatt gtggctggcc
      361 tcatcggagc gggcattccc gtccacgtct tcgccgacca gggcgcgcgc gccgacatgg
      421 agggcgtcgg cgccggcttc tcggatctct actggcgcca gccgtacacc gagatcgacc
      481 ccggcacgac gcgcccgacg agcggctggt ccgtcacctt cgccgggctc tacggcgagg
      541 acgtggcgcg ggaggtcgag ccgctgcgcc ccaggctgat cctccacgac acgctcatgc
      601 cggtcgcgcg cgtcgtggcc atccgtctgg gcctgccgcg cgtggtcatg cgcatgggcc
      661 aggcgttcga cccgcagaag ctccggcagc gctacgcgac ggacccgtcg atggcgccgt
      721 cggtctcgga caagtgccgg gaggccgtcc ggatcctgca ggaaaagcac ggcatcgccg
      781 aggcttcccc gttcctggct ttcagcgatg ccggcgggga gctcaacatc tactcggagc
      841 ccagccagtt catggccgcg cgtccgggcg agccgccgga caccgtcgag tacttcggct
      901 cgttgtggcc cgcgtcgcac agtgctgcgg acggggcgcc ctcgccgttc gggcccttgt
      961 cggaaggccg cgtccgggtg tacatctcgt tcggcacggt cgtctgggac gtgcatgcct
     1021 cggaggcgca ggcggccctg gaggcgctcg ccgacgccat cgcggagatg ccggcggtcg
     1081 cggcgctcat cagcctcggc ggaggcgact taccggcgct cgccgcgcgt ctcgcgcgcc
     1141 gcaacgtccg cgtcgagcgg tacgttgacc agatccgcgt gttgcgggac gcgtccgtct
     1201 tcgtgaccca tcacgggatg aactcgacgc acgaggcgat ctactacggc gtgccgatga
     1261 tctcctaccc gttcatctgg gaccagcccg gcatcgcggc ccgctgccag gagctcgggc
     1321 tcgccgttcc tcttgcgccg gagccgttgg cccgggtgtc cgttccggac gtgcggcgcg
     1381 cgctcgatcg gatcgcatca gggggcgacg agctgcgccg gcgcctggcc gaggcccggc
     1441 agtgggaact cgacgtcatc gcgcgccggc cggccgtgat cgagcgcatc aaggccctga
     1501 tggcctgaag ctggaggccg gctccggcca tggggacagc cgccgtccga tgtccgcgcc
     1561 tgctccaaca gccgccggtc ttgccggggc tgccggccgg gcggagggac gcccgccgcc
     1621 agccgcgcga gcgccgctgg cgcgtgagcg agcgcgccgg catcgcccgg gcgggccggc
     1681 gcgcggagcg aacgggtgga gtggcccgcc gacgcccgcg cggaactgaa cagcgtggct
     1741 cccggggagt gccgccgtga acaagcgagt ctatatcgcc gcgtatcacc agtccgagtt
     1801 cgggaagctc atgggcatga gcgtgcccga gatcgtcaag gcgtcggttg ccggcgtgtg
     1861 cggcgagatc gaggccgacc cctcgatcct cgacgtcggg tccgtcggcg ccacctgcac
     1921 gtacctcctg aaccagcagg ggctgctcgc cggcctgctc gccgcggtgc ccggactggc
     1981 cgggaagccg atcgaggccg tcgagaacgc ctgcgcatcc ggcggccagg cgatcctctc
     2041 ggtgatctac aagctgctgg tgggccaggg cgaggccggc atcgccgtcg ggttcgagaa
     2101 gatgcgggac gccgagggga agatggacgg ccgcctcatc ggccggaccc tcggcgtgtt
     2161 ctcgcacccc gacgagcggg agggcaaggt ccacatcttc ccgcacatct tcgccgaggt
     2221 gatggccgac tacatgacgc ggcacggcgt gaccgagcgc gatctcgcgc acgtctcggc
     2281 gacggagtac gcgaacgcca acagcaaccc gtacgcgcag atgaggaaga tcaaggtcac
     2341 cctggacgac tgcgcgaacc tcggcgaccg caaccggtac atcgtcgagg gcctgccgct
     2401 gaagacgtac gactgctcgc agatcaccga cgggtacgcc gccgtcatcg tcgccaccga
     2461 ggaaggcctg aagcgcctcg gcgtggcgaa ggccgacgcg gtggagattg cgggctacgc
     2521 gcaggcgacc gacagcctgc tccgggcgga tcgggacgtg ctccgggcgg aaggcgcgtt
     2581 cacggcgatg agcaaggcct acgcgatggc cgggctcacg ccggccgacg tcaacgtcgc
     2641 cgagatccac gactgcttca ccgtcatggg cgccatcggc accgaggtca tcgggaaggc
     2701 cccgttcggc cgcggcgcgc agtactgggt cgacgggcgg gcgcgcgcgg acggcgagtg
     2761 cggcatcaac acgtcaggcg gcctgattgc caagggccat cccgtcgggg ccaccggcgt
     2821 cgccatggtg ggctggtccg cgtggcagct cctcggaagg gtgccgccgg cgctgcaggt
     2881 gacgggcgcg aaggcggccg ctacgttcaa catcggcggc ccgatctgcg cgtcggtgtg
     2941 caccgtgctg aagccgggcg cgtga
//
LOCUS       DAOSIF010000404         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_29363_length_2937_cov_4.823040, whole genome shotgun sequence.
ACCESSION   DAOSIF010000404 DAOSIF010000000
VERSION     DAOSIF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29363_length_2937_cov_4.823040"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLT35_11685"
     CDS             complement(<1..383)
                     /locus_tag="PLT35_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG29930.1"
                     /translation="MDADSQRSVYDSPRMADGYAFDRPPVHPHIIKAVASRLAHRAPV
                     GRALDVGCGAGASTAALEPVARHAVGLEPVRRMLAHRRIVAPRASFVVAGAEHLPFQA
                     GAFDLVTAAGSLNYVDLRLFLPELSR"
     gene            complement(389..1336)
                     /locus_tag="PLT35_11690"
     CDS             complement(389..1336)
                     /locus_tag="PLT35_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOG29931.1"
                     /translation="MYTRRAVLAKASAAAALALSLLEGCARPARPVAAPAPPPDPVRA
                     PVDIVVQGAVDSELQPLLAALEGREQLQIAAWTFWRGRMAGQAVVVSRTEVGPVNAST
                     ATTLAIVNFRPRLIINQGTAGAVDPELRLFDIVAGEATVDYGAFRSAHADAGAGVDLS
                     RWTPMPHRLRLDGGERVAFERFPGDPAALAAALAIPNPRGRVVKGVIGSAFEFNREVD
                     RLIWVHKTYGAVSEDMESAFAAGTAAGFKVPFLAVRIISDSDHQDVEFQPVAGEHCAA
                     FVVELVKALPAPLAANTPAAAAERRCPSATPGALRTPPG"
     gene            complement(1370..>2937)
                     /locus_tag="PLT35_11695"
     CDS             complement(1370..>2937)
                     /locus_tag="PLT35_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HOG29932.1"
                     /translation="RAPARVSGAVTAALALALAFRLAGAAARPMHHDEANQAVKFGEL
                     LETGDYRYDRNDHHGPTLYYLTLPAAWARGQATLASLDERTLRAVPALFGAGLILLLV
                     PLARGLGRPAAAAAAMLAAVSPLLVYYSRFYIQESLFACFSLACLAAVGRFAERPSRA
                     AALCAGWCAGLAYATKETSILLLPAVAAAALLARGSAERDGSVQPAPAGDRRARRSAA
                     ALGLAAAVATAFVFYSSICKYPGGLLESVRSFGIYAARGAGAGPHAQPFGYYARMLAF
                     SSSGGLVWSEGLILGLAAAGMIASSRRTPGAGAGFWPRYVALYSLIAFLAFSAVPYKT
                     PWNAVPFHTGFVIMAGVGAAAVLEWLRSRLLKAVALLALAAAAAQLGVQSWRASFPYS
                     ADPRNPYVYAHTVPDFLRLVQRVRDVAARSPDGMRTPVKVVAGPYEQWPLPWYLRGMT
                     RVGYWTRAADADPLDEAPLIVASQEHAGEVGAALGDRYVQEFYGLRPEVVLTLFVERS
                     AWDRLIAARAAAP"
BASE COUNT          429 a         1067 c         1103 g          338 t
ORIGIN      
        1 cgggagagct ccggaaggaa caagcggaga tccacgtagt tgagcgatcc cgcggccgtc
       61 acgaggtcga acgcgccggc ctggaacggc agatgctcgg ccccggcgac aacgaacgac
      121 gcgcgcgggg cgacgatccg gcggtgtgcg agcatgcgcc gcacgggctc gagcccgacg
      181 gcgtgcctcg ccaccggctc gagggccgcc gtggaggcgc ccgcgccgca cccgacatcc
      241 agcgcgcggc cgacgggagc gcggtgcgcg aggcggctgg cgacggcctt gatgatgtgg
      301 ggatgcaccg gggggcggtc gaaggcgtac ccgtccgcca tccgcgggct gtcgtacacg
      361 ctgcgctggg agtcggcatc catgctcatc accccggagg agtccgcaac gcgccgggtg
      421 tcgcgctcgg gcacctcctt tccgcggccg ctgctggcgt gttcgccgcg agcggagccg
      481 gcagcgcctt cacgagctcg acgacgaacg cggcgcagtg ctcgccggcg acgggctgga
      541 actccacgtc ctggtggtcc gagtcggaga tgatgcggac ggcgaggaac gggaccttga
      601 agccggccgc cgtccccgcg gcgaacgcgc tctccatgtc ttccgacacg gcgccgtacg
      661 tcttgtgcac ccagatcagg cgatccacct cgcggttgaa ctcgaaggcc gacccgatca
      721 cccccttcac cacccgcccg cgcgggttcg ggatggccag cgcggcggcc agcgccgccg
      781 gatcgccggg gaaccgctcg aacgcgaccc gctctccgcc gtcgagccgc agcctgtgcg
      841 gcatcggcgt ccagcgcgac agatcgacgc cggcgccggc gtctgcatgc gcggaccgga
      901 acgcgccgta gtccaccgtg gcctcgcccg cgacgatgtc gaacaggcgc agttcgggat
      961 cgacggcgcc ggcagtgccc tggttgatga tcagccgggg gcggaagttc acgatggcga
     1021 gcgtcgtggc ggtggacgcg ttcaccggcc cgacttccgt gcgggacacg acgaccgctt
     1081 gtcccgccat gcgcccgcgc cagaaggtcc aggcggcgat ctggagctgc tcccgcccct
     1141 cgagcgcggc caagagcggc tgcagttcgc tgtcgacggc gccctgcacc acgatgtcca
     1201 cgggcgcgcg gacggggtcg gggggcggcg cgggcgcggc gacgggcctc gcgggcctcg
     1261 cgcacccctc gagcagggac agggcgagcg cggcggcggc gcttgccttc gccaggacgg
     1321 cgcgtctcgt gtacacggtc ggcattgtac cgttgacgcc cgtcccgcgt cagggcgcgg
     1381 cggcccgggc tgcgatcagc cggtcccagg cggaccgctc gacgaagagc gtcaggacca
     1441 cctcgggcct cagtccgtag aactcctgca cgtaccggtc gccgagcgcc gcgcccacct
     1501 cgccggcgtg ctcctgcgac gccacgatga ggggcgcctc gtcgagggga tcagcatcgg
     1561 cggcgcgcgt ccagtacccg acgcgcgtca tcccgcgcag gtaccagggc agcggccact
     1621 gctcgtacgg cccggcaacg accttgacgg gtgtccgcat cccgtccggg ctccgggccg
     1681 ccacgtcgcg cacgcgttgc accagccgca ggaagtcggg cacggtgtgg gcgtagacgt
     1741 acgggttccg cgggtccgcc gagtacggga agctggcgcg ccagctctgt acgccaagct
     1801 gcgccgcggc ggcggcgagg gcaagcagcg caacggcctt cagcagccgc gacctcagcc
     1861 actcgagcac ggccgcggcc ccgacgccgg ccatgatcac gaagccggta tggaacggca
     1921 ccgcgttcca cggcgtcttg tagggcacgg cggagaaggc caggaacgcg atgagcgagt
     1981 agagcgcgac gtagcgcggc cagaagccgg ccccggcccc tggcgtcctg cgcgacgatg
     2041 cgatcatgcc ggcggcggcg aggccgagga tcaggccctc gctccagacg agcccgcccg
     2101 acgacgagaa cgccagcatc cgcgcgtagt agccgaacgg ctgcgcgtgc ggtccggcgc
     2161 ccgccccgcg ggcggcgtag atgccgaacg accgaaccga ctcgagaagg cctccggggt
     2221 acttgcagat cgacgagtag aagacgaacg ccgtggccac ggcggcggcc agcccgagcg
     2281 ccgccgccga gcggcgcgcg cgccggtctc ctgccggcgc gggctgcacg ctgccgtcgc
     2341 gctccgccga tccccgggcg aggagcgcag ctgccgccac cgccggcagg aggaggatcg
     2401 acgtctcctt ggtggcgtag gccaggcccg cgcaccatcc ggcgcagagc gccgccgcgc
     2461 ggctcgggcg ctcggcgaac cggccgacgg cggccaggca ggccagcgag aagcacgcga
     2521 agagcgattc ctggatgtag aagcggctgt agtagacgag cagcggcgac accgccgcca
     2581 gcatcgcggc ggcggccgcc gccgggcgcc caagccccct cgccagcggt acgagcagca
     2641 ggatgaggcc ggcgccgaag agcgccggca cggcgcgcag agtgcgctcg tcgagcgacg
     2701 cgagcgtggc ctggccgcgc gcccacgccg cgggcagcgt caggtagtag agcgtggggc
     2761 cgtgatggtc gttccggtcg tagcgatagt cgccggtttc gagcagttcg ccgaacttga
     2821 cggcctggtt ggcctcgtcg tggtgcatcg gccgcgcggc ggccccggcc aggcggaacg
     2881 ccagcgcgag cgccagcgcc gccgtgaccg cgccgctgac cctggccggg gccctgt
//
LOCUS       DAOSIF010000405         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_29656_length_2915_cov_3.589161, whole genome shotgun sequence.
ACCESSION   DAOSIF010000405 DAOSIF010000000
VERSION     DAOSIF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29656_length_2915_cov_3.589161"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLT35_11700"
     CDS             <1..255
                     /locus_tag="PLT35_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HOG29933.1"
                     /translation="FGLLAFIGGKMIHEAFGRPEEQADACDPTKGWRLVMLSIATSID
                     ALAGVPQLFAPLVHICTESPCSERFDLVRTAKRHRYTFRV"
     gene            297..2015
                     /locus_tag="PLT35_11705"
     CDS             297..2015
                     /locus_tag="PLT35_11705"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOG29934.1"
                     /translation="MYVRTTPRRNKDGSVVRYLQLAHNVWDPVKKRSRTEVLYNLGRE
                     DEASRAALERLAGSLQRFLSAAPEDPQGNLFAAAEGFDFLGSRPLGGSYALDGLWRRL
                     GIDAALRRLLKGRRRDPAAERVLFALVANRALAPSSKLAAARWISEDACIDGLPLATD
                     DACYRAMDWLLEVQGELEREVFHQVASLLSLEVDLLFFDTTSTYFETDEADEPLARDE
                     RGRPLPAGAEEEGARGAAGFRSYGRSKDHRPDLPQVVVGMAVTREGIPVRVWSWPGNT
                     SDSALIREAKEDLREWTLSGVVWVADRGFTSQENRRALRCGDEHYIMAEKLRGGSAEA
                     KAALSRAGRYAELPGGLRVKEVRVSARERFVVCHHPESAERDAAVRGRLVARLTDMIA
                     GSDKLAPEKRAELRGAIGTKPGLKRYLRTTPGGLLRIDEAAVRAEERLDGKLLLRSSD
                     PTLSAEDIALGYKQLWQVERGWRDMKQVLDLRPACHRREDRIRAHVVLCWLALLLVRA
                     CENSCGDTWPNLRRELERLKLGSFSGPAGRFRQRSELTAGQRTILTRLGLAEPPRMQD
                     LAAAPS"
     gene            2088..2333
                     /locus_tag="PLT35_11710"
     CDS             2088..2333
                     /locus_tag="PLT35_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HOG29935.1"
                     /translation="MDYRGGQLRNGSLAVGLSLAILGIDIWYPALVIGVVAGALTVVG
                     LELGARFGALLGRRMEVVGGLILIAIGVKILIEHLTG"
     gene            complement(2438..>2915)
                     /locus_tag="PLT35_11715"
     CDS             complement(2438..>2915)
                     /locus_tag="PLT35_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29936.1"
                     /translation="GRLREKLAVRGAQLGLFAGLLLLLGRFGLLGYALAFVLSEIAVH
                     IALSWRMTKDFCMGRSAIISAYRPGLVGGVCACAALWGESWAGRWLGLGSGLVLVVQA
                     ATGAAVLLVAVLCVARGLVLREVASRGAGTVPGRVAARLVSWRYHGALGDTAEADV"
BASE COUNT          454 a         1080 c          972 g          409 t
ORIGIN      
        1 ttcggcctgc tcgccttcat cgggggcaag atgatccacg aggcgttcgg gcgtccagag
       61 gagcaggcgg acgcctgcga ccccaccaag ggctggcggc tcgtcatgct gtcgatcgcg
      121 accagcatcg acgccctcgc tggcgttccg cagctattcg cccctctggt ccacatctgc
      181 acggaaagtc cctgctcaga gcgttttgac ctggtcagaa ccgcgaaaag gcatagatac
      241 acgtttaggg tttgagtact tgatttagct ggtagttgtg tgtaaatctc tagtccatgt
      301 acgtgcgcac gacaccgcgc aggaacaagg acggctcggt ggtccgctac ctgcagctcg
      361 cgcacaacgt ctgggacccg gtgaagaagc gctcgcgcac cgaggtgctc tacaacttag
      421 gccgcgagga cgaggcgagc cgggcggccc tcgagcgcct cgccggctcg cttcagcgct
      481 tcctctcagc cgcccctgag gaccctcagg gcaacctctt cgcggcggcc gagggcttcg
      541 acttcctcgg atcgcggccc ctcggcggca gctacgccct cgacggcctc tggcggcgcc
      601 tcggcatcga cgcggcgctg cgccgcctcc tcaaggggag acgccgcgac ccggccgccg
      661 agcgcgtgct cttcgccctg gtcgcgaatc gcgccctggc gccgtcctcg aagctggccg
      721 cggcgcgctg gatcagcgag gacgcctgca tcgacggcct ccctctggcc acggacgacg
      781 cctgctaccg cgccatggac tggctgctcg aggtccaggg cgagctcgag cgcgaggtct
      841 tccaccaggt cgccagcctg ctcagcctcg aggtcgatct tctgttcttc gacacgacct
      901 cgacctactt cgagacggac gaggccgacg agccgctggc ccgcgacgag cgcgggcggc
      961 cgctgccggc cggcgcggag gaggaaggcg cccgcggcgc cgccggcttc cgcagctacg
     1021 gacgctccaa ggatcaccgt cccgacctgc ctcaggtggt ggtcggcatg gccgtgacgc
     1081 gtgagggcat cccggtgcgc gtctggtcct ggccggggaa cacgagcgac tcggcgctca
     1141 tccgcgaggc gaaagaggac ctgcgcgagt ggacgctctc aggcgtggtt tgggtggccg
     1201 accgcggctt cacgagccag gagaaccgcc gcgccctgcg atgcggcgac gagcactaca
     1261 tcatggccga gaagctgcgc ggagggtccg cggaggccaa ggccgccctc tctcgcgccg
     1321 gccgctacgc cgagctcccc ggcggcctgc gcgtcaagga ggtgcgtgtg tccgcacgcg
     1381 agcgcttcgt cgtctgccac caccccgaga gcgccgaacg tgacgccgcc gtccgcgggc
     1441 gcctcgtcgc ccgcctcacc gacatgatcg ccggctccga caagctcgcg ccggagaagc
     1501 gcgccgagct gcgcggggcg atcgggacga agcccggcct gaagcgctac ctgcgcacca
     1561 cgcccggcgg cctcctgcgc atcgacgagg cggccgtgag ggccgaggag cggctcgacg
     1621 gcaagctgct cctgcgctcc tcggacccca cgctctccgc cgaggacatc gccctcggct
     1681 acaagcagct ctggcaggtc gagcgcggct ggcgggacat gaagcaggtg ctcgacctca
     1741 ggcccgcgtg ccaccgccgc gaggaccgca tccgcgccca cgtcgtgctc tgctggctgg
     1801 cccttctcct ggtgcgcgcc tgcgagaaca gctgcgggga cacctggccg aacctgcgcc
     1861 gcgagctcga gaggctgaag ctgggcagct tctccggtcc cgccggccgc ttccgtcagc
     1921 gcagcgagct cacggccggc cagcgcacga tcctcacgag gctcgggctc gccgagccgc
     1981 cacggatgca ggacctcgcg gccgccccct cctgagcccc tcgcggcata gatacacgcc
     2041 gtctgcgcgc tcaagcccgc cttttgcctg caaaaccggc ctgtttcgtg gactatcgcg
     2101 ggggacagct gcggaacggc agcctcgccg tcggcctctc gctcgccatc ctgggcatcg
     2161 acatctggta ccctgcgctg gtgatcggcg tcgtggccgg cgcgctcacc gtggtcggcc
     2221 tcgagctcgg ggcgcggttc ggcgctctgc tggggcgccg catggaggtg gtgggcggcc
     2281 tgatcctcat cgcgatcggc gtcaagatcc tcatcgaaca cctgaccggg tagtcgcccc
     2341 ggcagacccg tgccactcgc gatgacgcgc gggaacgcac gaacgtgagc gggatgctca
     2401 tgaccgcggc tcaggcgacc ggcgcctacg agcgccctca cacgtcggcc tcagccgtgt
     2461 ctccgagagc gccatgatac cgccacgaca ccagccgcgc cgcgacgcgg ccgggaacgg
     2521 tccctgcgcc tcgtgacgcc acctcgcgca acacgagacc ccgcgcgacg cacaagaccg
     2581 ccaccagaag gactgccgcg ccggtggctg cttggacgac caggacgagg ccagacccca
     2641 gtccaagcca gcggccggcc cacgattccc cccagagcgc cgcacaggcg cagacaccgc
     2701 cgaccagacc cggacgatac gcgctgatga tcgcgctcct gcccatgcag aagtccttcg
     2761 tcatgcgcca ggagagcgcg atgtgcaccg cgatctcgga caacacgaat gcaagagcgt
     2821 agccgagcaa cccgaatcga cccaggagca ggagaaggcc ggcgaacagc cccagttggg
     2881 cgccgcgcac ggccagcttc tccctcaggc gcccg
//
LOCUS       DAOSIF010000406         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_29666_length_2914_cov_3.942637, whole genome shotgun sequence.
ACCESSION   DAOSIF010000406 DAOSIF010000000
VERSION     DAOSIF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29666_length_2914_cov_3.942637"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLT35_11720"
     CDS             complement(<1..516)
                     /locus_tag="PLT35_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29937.1"
                     /translation="MPPEFAIRSDAVDVDEIMKQIRARIREKRGVDYTEEEIRQLAGV
                     KLEKFLDPTRVRSDLVQQYRRAHDPAAVPTLEPPPENYAFEDYHVYGSARGIGGRLIA
                     AIRKMLNPVLKLFINPNPIIHVLHRQSAINTHNERQFERIERTRTDLAALNYEVIHNL
                     VVEMTRLAVEVK"
     gene            complement(518..1474)
                     /locus_tag="PLT35_11725"
     CDS             complement(518..1474)
                     /locus_tag="PLT35_11725"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOG29938.1"
                     /translation="MARRPTPEDPAPGAAARGGAGRRSAPALLFLSLRPGQWTKNLVV
                     FAGLIFGRELFRPVSVWRAVAAFAVFCALSGAVYLINDVMDREADRRHPLKARRPIAS
                     GALAPGLAAAAAAALAAFALAAAFALGAPFGSIALGYVLLQLAYSGPLKHIVIVDVLA
                     IAIGFVLRAVAGVVVVGVPISHWLLVVTILLALFLSLSKRRHELVLLAGGATEHRPIL
                     GEYSPYLLDQMISVVTASTLVAYIFYCISPETVQKFGTGLLGLTIPFPLYGIFRYLYL
                     VHRREGGGNPSQMLLTDRPLLACVALWAAAVVVIVYLRPFGA"
     gene            complement(1495..1836)
                     /locus_tag="PLT35_11730"
     CDS             complement(1495..1836)
                     /locus_tag="PLT35_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG29939.1"
                     /translation="MQIEERKIGNVIILDLKGKLTLGDGDELLRSTVSRLVQAGSKQI
                     VLNLGDVPYIDSAGLGEVVRTYTTVKKQDGMLKLLNLTKRIQDLLAITKLLTVFETFD
                     NEAEAVVSFSA"
     gene            complement(1974..2285)
                     /locus_tag="PLT35_11735"
     CDS             complement(1974..2285)
                     /locus_tag="PLT35_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HOG29940.1"
                     /translation="MAFQDRTLTCVECGASFTWTAGEQSFYADKQFTNEPKRCRACKG
                     KRNQRRASEPGADRDRIDTRSTCSACGRETTVPFKPTQGRPVYCRECYQQRKPRGGPA
                     A"
     gene            complement(2350..2547)
                     /gene="rpsU"
                     /locus_tag="PLT35_11740"
     CDS             complement(2350..2547)
                     /gene="rpsU"
                     /locus_tag="PLT35_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG29941.1"
                     /translation="MAEVKVQDGESIESALRRFKRKVQQEDIIKDIKKHSFYLKPGDK
                     RRAKQALARKRSRKKLRRETE"
     gene            2702..>2914
                     /locus_tag="PLT35_11745"
     CDS             2702..>2914
                     /locus_tag="PLT35_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HOG29942.1"
                     /translation="MRVLVVGGGAREHALCWKLGGERGASEIVCAPGNAGIARDFRCL
                     PADAGRPADVVALVERERIDLTVVGPE"
BASE COUNT          444 a         1014 c         1002 g          454 t
ORIGIN      
        1 cttcacctcg acggccaagc gcgtcatctc gacgacgagg ttgtggatca cctcgtaatt
       61 cagcgccgcc aggtccgtcc gcgtgcgctc gatccgctcg aactgcctct cgttgtgcgt
      121 gttgatggcg ctctggcggt gcaggacgtg gatgatcggg ttcgggttga tgaacagctt
      181 gagcaccggg ttgagcatct tccggatggc cgcgatcagc cgccccccga tgcctcgagc
      241 cgatccgtag acgtggtagt cctcgaaggc gtagttctcc ggcggcggct cgagcgtggg
      301 cactgcggcc gggtcgtgcg cccgacggta ctgctgcacc aggtccgagc gcacccgcgt
      361 cggatcgagg aacttctcga gcttgacgcc ggccagctgc cggatctcct cctccgtgta
      421 gtccacgccg cgcttctcgc ggatccgcgc gcggatctgc ttcatgatct cgtcgacgtc
      481 gaccgcatcg gaacggatgg cgaactccgg tggcatgtca ggctccaaag ggcctgaggt
      541 agacgatcac cacgaccgcc gccgcccaga gcgcgacgca cgccagcagc gggcggtcgg
      601 tcagcagcat ctgcgacggg ttcccgcccc cctcgcgccg gtgcacgagg tagaggtagc
      661 ggaagatgcc gtagagcggg aaggggatcg tcagcccaag caggccggtc ccgaacttct
      721 gcacggtctc gggactgatg cagtagaaga tgtacgcgac cagcgtggac gcggtcacga
      781 cggaaatcat ctggtcgagc aggtaggggc tgtactcgcc gaggatcggc cggtgctcgg
      841 tggccccgcc ggcaagcagc accagctcgt ggcgccgctt gctgagcgac aggaacagcg
      901 ccagcaggat ggtgacgacg agcagccagt ggctgatggg cacgccgacg accacgacgc
      961 ccgccaccgc ccgcagcacg aagccgatgg cgatcgccag cacgtcgacg atcacgatgt
     1021 gcttgagcgg ccccgagtag gcgagttgaa gcaggacgta acccagggcg atggagccaa
     1081 agggcgcccc gagggcgaag gcggcggcca gggcgaaggc ggcgagcgcg gccgctgccg
     1141 cggccgccag gcccggcgcg agcgccccgg acgcgatcgg ccggcgggcc ttcagcgggt
     1201 gccggcggtc cgcctcccga tccatcacgt cgttgatcag gtacaccgcg ccggagaggg
     1261 cgcagaacac ggcgaaagcg gccaccgcgc gccacaccga cacggggcgg aagagctccc
     1321 gcccgaagat caggccggcg aagacgacga ggttcttcgt ccactggccc gggcggagcg
     1381 agaggaacag gagggcgggc gcagaccgcc gccccgcacc ccctcgagcg gcggcgccag
     1441 gggcggggtc ttccggcgtg ggacgacgcg ccatgtagcg cgaagacccg ggcgtcaggc
     1501 gctgaagctg acgaccgctt cggcctcgtt gtcgaacgtc tcgaacacgg tcagcagctt
     1561 ggtgatggcc agaaggtcct ggatccgctt cgtcagattc aggagtttga gcatgccgtc
     1621 ctgcttcttg accgtcgtgt acgtgcggac gacctcgccg aggcccgcgc tgtcgatgta
     1681 cggcacgtcg ccgaggttca gcacgatctg cttcgagccg gcctggacga gccggctgac
     1741 cgtgctgcgc agcaactcgt cgccgtcacc cagcgtcaac ttgcccttca agtcgaggat
     1801 gatgacgttg ccgatcttcc gttcttcgat ctgcatcgct gatccctttc tcactgccta
     1861 ccgggccgcc cgcccgcgcg gccgggcgcc ccggaacatg gaccgcagtc agggcctgca
     1921 tcctaacgca ggtcccacgc acggtcaacg attgcagcca gccccgccgg acctcaggcg
     1981 gcgggaccgc cgcgcggctt gcgctgctgg tagcactcgc ggcagtacac cggacggccc
     2041 tgcgtcggct tgaacgggac ggtcgtctcc ctcccgcacg ccgagcacgt ggagcgcgtg
     2101 tcgatccgat cgcggtcggc gccgggctcg ctcgcccgcc gctgattccg cttgcccttg
     2161 caggcccggc agcgcttggg ctcgttggtg aactgcttgt ccgcgtaaaa cgactgctcg
     2221 ccggccgtcc acgtgaacga ggcgccgcac tcgacgcacg tcagagtccg gtcctggaac
     2281 gccatctggt gcaccggcct ccgggattcg cgcacccggg ccgcgcggcc cgggtgctgg
     2341 cccttcgccc tactcggttt cgcggcggag cttcttccgg ctccgcttcc gcgccagcgc
     2401 ctgcttggcg cggcgcttgt cgccaggctt caaatagaag gagtgcttct tgatgtcctt
     2461 gatgatgtct tcctgctgga ccttccgctt gaaccggcgg agcgcgctct caatcgattc
     2521 gccgtcctgg accttgactt cagccacgcg tcacacaccc ccctccgtgg cccgttcgac
     2581 gcacgtctcc acggccaggg agcgtcccgg agccgtggtg cgaatatgct aggctaacac
     2641 agcttgcggc cggggcggcg ggaaacgccg ccccggccgc gcccgggagg tgcgcccggt
     2701 catgcgagtt ctcgtggtgg gcggcggggc ccgcgagcac gcgctctgct ggaagcttgg
     2761 cggcgagcgc ggcgcctcgg agatcgtctg cgcccccggc aacgccggca tcgcccgcga
     2821 cttccgctgc ctgcctgccg acgccggcag gccggccgac gtggtcgccc tggtcgagcg
     2881 cgagcgaatc gacctgaccg tcgtcggccc ggaa
//
LOCUS       DAOSIF010000407         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_29786_length_2904_cov_3.089154, whole genome shotgun sequence.
ACCESSION   DAOSIF010000407 DAOSIF010000000
VERSION     DAOSIF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29786_length_2904_cov_3.089154"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLT35_11750"
     CDS             <1..536
                     /locus_tag="PLT35_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29943.1"
                     /translation="VDAGVHHLQREAKHVKPADRLALLQGLYRDKVALRRRHEAGAAL
                     VASYDANNAYQFAIAREEAHLDWLRRAITAMGGVPDDSADPPPLPVPRGRADAVRTVA
                     GDDARLAAEFESRWRDRVAACSQARDRKMLELLLGEALEHQRFFAQAASGDTDLLGRR
                     PVGAGTPGDVLAARWVG"
     gene            542..1141
                     /gene="folK"
                     /locus_tag="PLT35_11755"
     CDS             542..1141
                     /gene="folK"
                     /locus_tag="PLT35_11755"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOG29944.1"
                     /translation="MPVHAAVALGSNLGDRLAALDGASRALARLLDGVVISRFIETAA
                     EGVGEQPPFLNGAAVGLWGGGARALLEALLAIERDAGRERPYSGAPRILDLDLILFGS
                     AVIDEPGLAVPHPRFRERGFVLGPLSEIAPNLVDPVTGATVAALHRAWLAQSARRGGP
                     DPLPGPAIRLPGSGEPALSARMTVCEPRPPAGGGRISEA"
     gene            1151..1399
                     /locus_tag="PLT35_11760"
     CDS             1151..1399
                     /locus_tag="PLT35_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29945.1"
                     /translation="MARGFDSKSVEYQQEEAQRSRERMRERELTREEVARVQRCRTLE
                     LAHARAAADLGRAAAPAQREMLQQALAALESELASLAR"
     gene            complement(1396..1866)
                     /locus_tag="PLT35_11765"
     CDS             complement(1396..1866)
                     /locus_tag="PLT35_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29946.1"
                     /translation="MTRRFVFVALLAAALALPALAGAQPANFSGKWIQDMEKSDPMGG
                     GRGPAGPQTVTITQTATELTLERETPNGVMKTVYKLDGSPSVNPGGRGGEITSTSAWE
                     GSKLVTKYTRTMGQNSMEVVETRSLEADGTLLLVTAMKGGPMGDTTRKTVFKKS"
     gene            complement(1888..>2904)
                     /locus_tag="PLT35_11770"
     CDS             complement(1888..>2904)
                     /locus_tag="PLT35_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG29947.1"
                     /translation="MAMGVRQGSGASNTLTPEDAAAIRNQIPTAQYVAAGVSTRNQII
                     AGHQNWQTRIQGTDVDLPLMRSWPMKYGLFFTPQDVTTAAKVVVLGAVVSTNLFGEET
                     DPTGQIVRIKNQPFKVIGVLASKGAGTGFEDQDDTAMVPYTTAQKKLMGIQHIQNVTI
                     SASSAHEVKATAEAIAQLLRTRHKIMDGNNDDFMVRTMEEMAAMRTETTKTMTALLAG
                     IAGVSLLVGGIGIMNIMLVSVTERTREIGLRMAIGARGSDVLTQFLVEAVVLSLFGGS
                     IGIALGFGISWGVTEFLTWPTTISSEAVLLSFGFSAIIGIFFGFYPATKAAKLDPIEA
                     LRFE"
BASE COUNT          400 a         1004 c         1024 g          476 t
ORIGIN      
        1 ccgtggacgc gggtgtgcat cacctgcaaa gagaagcaaa gcacgtgaag cccgcggacc
       61 gcctggcgct gctccagggc ctgtaccggg acaaagtggc gctccgccgg cgccatgagg
      121 ccggcgcggc gcttgtcgcc tcctacgacg cgaacaacgc ctaccagttc gccatcgcac
      181 gcgaagaggc ccacctcgac tggctgcgcc gcgccatcac ggccatgggc ggggttcccg
      241 acgactcggc ggacccgccg ccgctgccgg ttccgcgcgg ccgcgccgac gccgttcgga
      301 ccgtcgccgg cgacgacgcc aggctggcgg ccgaattcga gtcccgctgg cgcgacaggg
      361 tggccgcctg cagccaggcg cgggaccgga aaatgctgga actcctgctg ggcgaggcgc
      421 tcgaacacca gcggttcttc gcccaggccg catccggaga caccgacctg ctcgggaggc
      481 ggcccgtcgg ggccggcacc ccgggcgacg tgctcgcggc tcgctgggtt gggtgactcg
      541 gatgcccgtc cacgccgcgg tcgccctggg aagcaacctc ggcgatcggc tcgcggcgct
      601 ggacggggcc tcccgcgcgc tggcgcgcct ccttgacggc gtcgtcattt cccgtttcat
      661 cgagaccgcc gcggaaggcg tcggcgagca gccgccgttt ctcaacggcg ccgcagtcgg
      721 cctctggggg ggcggtgcgc gcgcgctgct cgaggcgctc ttggccatcg agcgggacgc
      781 cggccgcgag cggccctact cgggcgcgcc ccgcattctc gacctggact tgatcctctt
      841 cggctcggcg gtgatcgacg agcccggcct cgcggttccg catccccgct ttcgcgagcg
      901 cggcttcgtg ctcggtccgc tgtccgagat cgcgccgaac ctcgtcgatc ccgtcacggg
      961 ggcgaccgtg gccgccctcc accgcgcgtg gctcgcgcaa tcggctcgga gaggcgggcc
     1021 tgacccgctg ccagggccgg cgatccgcct gcctggctcc ggcgagccgg cactttcggc
     1081 gcgaatgacc gtctgtgaac cgcggccccc ggctggcggg gggcgcatat cggaggcgta
     1141 gacagcagcg atggcccgag gattcgacag caagtcggtg gagtaccagc aggaggaggc
     1201 gcagcgcagc cgcgagcgga tgcgcgagcg cgaactgacg agggaggaag tggcgagggt
     1261 ccagcgctgc cggacgctcg agctggcgca cgcccgcgcg gcagccgatc ttggaagagc
     1321 cgccgcgccc gcgcagcgcg agatgctgca acaggcgctg gcggccctcg agtcggagct
     1381 ggcgtcgctg gcgcgctagc tcttcttaaa cacggttttg cgggtggtgt cgcccatcgg
     1441 cccgcccttc atcgccgtca cgaggagcag ggtcccgtcg gcctccaggc tgcgggtctc
     1501 gaccacctcc atcgagttct gccccatggt gcgggtgtac ttcgtgacga gcttcgagcc
     1561 ttcccaggcg ctcgtgctgg tgatctcgcc gccgcgaccg ccggggttga cgctcgggct
     1621 gccgtcgagc ttgtagacgg tcttcatcac gccgttgggc gtttcgcgct ccagcgtgag
     1681 ttcggtggca gtctgggtaa tggtcaccgt ctgcgggccg gcggggccgc ggccgccgcc
     1741 catcgggtcg ctcttctcca tgtcctggat ccacttcccg gagaagttgg cgggctgggc
     1801 tccggcgagg gccggcaggg ccagggccgc ggcgaggagg gctacgaaca cgaaccgtct
     1861 tgtcatggcg gactccttgg ttgtgagtta ctcgaatctg agcgcctcga tcggatcgag
     1921 cttggcggcc ttggtggccg gatagaagcc gaagaagatg ccgatgatcg ccgagaagcc
     1981 gaacgacagc aggaccgcct cgctcgagat cgtggtcggc caggtgagga actcggtcac
     2041 gccccaggag atgccgaacc cgagcgcgat gccgatgctg cccccgaaca ggctgagcac
     2101 caccgcctcg acgaggaact gcgtcagcac gtcggatccg cgcgcgccga tggccatgcg
     2161 aaggccgatc tcgcgggtgc gctcggtcac cgacacgagc atgatgttca tgatcccgat
     2221 gccgcccacg aggagcgaga cgccggcaat gccggcgagc agggccgtca tcgtcttcgt
     2281 ggtctccgtg cgcatggccg ccatctcttc catcgtccgg accatgaagt cgtcgttgtt
     2341 gccgtccatg atcttgtggc gcgtgcgcag cagctgcgcg atggcttcgg ccgtggcctt
     2401 cacctcgtgc gccgacgacg ccgagatggt cacgttctgg atgtgctgga tgcccatcag
     2461 cttcttctgc gccgtggtgt agggcaccat cgccgtgtcg tcctggtctt cgaaccccgt
     2521 gcccgcgccc ttgctcgcga gcacgccgat caccttgaag ggctggttct tgatccgcac
     2581 gatctggccc gtcgggtccg tttcctcccc gaagaggttc gtggacacga ccgccccgag
     2641 cacgacgacc ttcgcggcgg tcgtcacgtc ctggggcgtg aagaacaggc cgtacttcat
     2701 cggccaggag cgcatgaggg gcaggtcgac gtcggtaccc tggatgcgcg tttgccagtt
     2761 ctggtggccc gcgatgatct ggttccgcgt gctcactccg gccgcgacgt actgcgccgt
     2821 gggaatctgg ttgcggatgg ccgccgcgtc ctcgggcgtg agggtgttcg acgcccccga
     2881 gccctgacgc acgcccattg ccat
//
LOCUS       DAOSIF010000408         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_29986_length_2890_cov_3.193651, whole genome shotgun sequence.
ACCESSION   DAOSIF010000408 DAOSIF010000000
VERSION     DAOSIF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29986_length_2890_cov_3.193651"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PLT35_11775"
     CDS             complement(<1..1059)
                     /locus_tag="PLT35_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG29948.1"
                     /translation="MQPSDSRKTPLTFDTIEPGSGLAAALAKSRADIIGEVSASNLKG
                     RGGAGFPTGTKWNLAAAARVHGEAAKYVICNADEGEPGTFKDRVLLTAYADLVFEGMT
                     IAARAIGATKGLLYLRGEYVYLRPHLEAVLDRRRKAGQLGADILGTPGFEFDIEIRMG
                     AGAYVCGEETALIESLEGHRGEPRNRPPFPVNTGFMGQPTVVNNVETLASVTAIFAKG
                     AAWFRGIGTEKSSGFKLFSVSGDCDAPGVYEFPLGITIAELLERVGGTGAKAVQVGGA
                     SGQAVPASQFGRRIAFEDLATGGSIIVFGPGRDMLHVAKNFMEFFVEESCGQCTPCRA
                     GNAKLLEGIERLERGACSS"
     gene            complement(1075..1587)
                     /gene="nuoE"
                     /locus_tag="PLT35_11780"
     CDS             complement(1075..1587)
                     /gene="nuoE"
                     /locus_tag="PLT35_11780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG29949.1"
                     /translation="MLVSQQQKLQEEIRALVARHGRTRAALMPVLQELQEKYASITDF
                     AMQVVADELGIHPSEVYGVVSFYAFLHHEPKGRFQIRLCQTISCDLAGKARVAKQLEN
                     ELGIAFGETTPDGRFSLEWASCLGMCDQGPALLVNDRVYTRVTAEQAHRIIEECRSVF
                     GAHATQAAHA"
     gene            complement(1581..>1833)
                     /locus_tag="PLT35_11785"
     CDS             complement(1581..>1833)
                     /locus_tag="PLT35_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29950.1"
                     /translation="MVLDVMMEQPDDGIAMAQDLRRAGVTIPIVMLTSVGKVTGLDYG
                     RDNDLVPVDAFFEKPVRPEDLLKTVNGLLKQGTQGDTTC"
     assembly_gap    1834..1843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2282..>2890)
                     /locus_tag="PLT35_11790"
     CDS             complement(2282..>2890)
                     /locus_tag="PLT35_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG29951.1"
                     /translation="AEGYAGPLTPEQQDVVVRAQRRLASLHELIDDLLDLAAGKAGLE
                     QSAPERLDLGGLVAEIVDRFRPVGAAKGVALTLDQPPEALHLIADRRDLERVFVNLVG
                     NAVKYTARGEVRVRMQRGDGRIRVDVADSGIGIPKSALPFLFNEFYRAANAKAGGEPG
                     TGLGLAIVKLLVVRHGGEIAVESEEGRGTTMTVTWPAAPDLA"
BASE COUNT          390 a         1066 c          976 g          448 t
ORIGIN      
        1 cgacgagcac gcgcctcgct ccagacgctc gatcccctcg agcagcttgg cgtttcccgc
       61 gcggcacggc gtgcactggc cgcaggactc ctcgacgaag aactccatga agttcttggc
      121 cacgtgcagc atgtcgcggc cgggcccgaa cacgatgatc gatccgccgg tggcgaggtc
      181 ctcgaaggcg atccggcggc cgaactgcga cgccgggacc gcctgccccg aggccccgcc
      241 gacctgcacc gccttggcgc cggtcccgcc gacgcgctcg agcagctcgg cgatcgtgat
      301 cccgagcggg aactcgtaca cccccggcgc gtcgcagtcg cccgaaacgc tgaacagctt
      361 gaagcccgag gacttctccg tgccgatgcc gcggaaccag gcggcgccct tcgcgaagat
      421 ggccgtgacc gacgcgagcg tctcgacgtt gttcacgacg gtcggctgcc ccatgaaccc
      481 ggtgttcacc gggaacggtg gccgattccg cggctcgccc cggtgcccct cgagcgactc
      541 gatgagcgcc gtctcctcgc cgcacacgta ggcgccggcg cccatgcgga tctcgatgtc
      601 gaactcgaac cctggcgtcc cgaggatgtc ggccccgagc tgccccgcct tccgccgccg
      661 gtccagcacg gcctccaggt gcggccgcag gtagacgtac tcgccgcgca ggtagaggag
      721 gcccttggtc gcgccgatgg cccgggcggc gatggtcatg ccttcgaaca ccagatcggc
      781 gtaggccgtg agcagaaccc ggtccttgaa cgtgccgggc tcgccctcgt ccgcgttgca
      841 gatgacgtac ttggcggcct cgccgtgcac gcgcgccgcc gcggcgaggt tccacttcgt
      901 gcccgtcggg aacccggcgc cgccccgccc tttcaggttc gacgccgaca cctcgccgat
      961 gatgtcggcg cggctcttcg ccagcgcggc cgccaggcca gagcccggct cgatcgtgtc
     1021 gaaggtgaga ggggtcttcc ggctgtcact gggctgcatc tcggcctcct ccccctacgc
     1081 gtgcgcggcc tgcgtcgcgt gcgccccgaa cacgctgcgg cattcctcga tgatccggtg
     1141 cgcctgctcg gcggtaacgc gggtgtagac ccggtcgttc accaggagcg ccgggccctg
     1201 atcgcacatg cccaggcagc tggcccactc gaggctgaag cgcccgtccg gcgtcgtctc
     1261 gccgaaggcg atgcccagct cgttctcgag ctgcttcgcc acgcgcgcct tgccggccag
     1321 gtcgcacgag atggtctggc agagccggat ctggaagcgg cccttgggct cgtggtgcag
     1381 gaaggcgtag aaggacacga cgccgtagac ctcggacggg tggatgccaa gctcgtcggc
     1441 caccacctgc atcgcgaagt ccgtgatgga cgcgtacttc tcctgcagct cctggagcac
     1501 cggcatcagc gccgcacgcg tccggccgtg gcgggcgacg agcgcgcgga tctcctcctg
     1561 cagcttctgt tgctggctga ctagcatgtc gtgtctccct gcgtcccctg cttcagcagc
     1621 ccgttgaccg tcttcagcag gtcttcgggc cggaccggct tctcgaagaa cgcgtccacg
     1681 ggcaccaggt cgttgtcgcg gccgtagtcg agccccgtca ccttgcccac gctcgtcagc
     1741 atgacaatgg ggatcgtcac gcccgcgcgg cgcaggtcct gcgccatggc gatgccgtcg
     1801 tccggctgct ccatcatcac gtcgagtacc atcnnnnnnn nnncgagggc ggccatccct
     1861 tcgtcccggt tcgccgcggt ccggacctcg tgctgctccc gcttcaggaa gagcgacagc
     1921 gcgaaggtga tatcggggtc gtcgtccact acgaggatct ttgccattga aacgctccct
     1981 ggtcggtgcg gtccgcggag atccgggcgc ccggcgccag ccggtcctcg gcccctgcat
     2041 cacacgggtc catccctcgc gcaccggccg ccggcggcgc cggcgcgcgg ctgtggatcg
     2101 gactccgcgg caacgctcgg ggattgacgg gcggcgtgcg gggatccagc cgggggcctc
     2161 atcggccggc ggcgcgaccg cctgaggggg cggctggcag cctcgcccgg ccacgctcat
     2221 catagccggg ggccgacgga ggaatcaact cgccttcgcc cgcgtccggc aagcggccgc
     2281 cctaggcgag atccggcgcg gcgggccagg tcaccgtcat cgtcgtcccc cgcccttcct
     2341 cgctctcgac ggcgatctcc ccgccgtggc gcacgacgag cagcttcacg atggcgagtc
     2401 caaggccggt ccccggctcg ccgccggcct tggcgttcgc tgcgcggtag aactcgttga
     2461 acaggaacgg cagggccgac ttcgggatgc cgatgcccga atcggcgacg tcgacgcgaa
     2521 tgcgcccgtc gccccgctgc atccgcaccc ggacctcgcc cctggcggtg tacttgacgg
     2581 cgttgcccac gaggttcacg aacacgcgct cgaggtccct gcgatccgcg atgaggtgga
     2641 gcgcttcggg cggctgatcc agcgtcaggg ccacgccttt tgccgcgccg acgggccgga
     2701 agcggtcgac gatctcggcg acgagcccgc cgagatcgag gcgctcgggc gccgactgct
     2761 ccaggccggc cttgccggcg gccagatcca gcaggtcgtc gatgagctcg tggaggctcg
     2821 cgaggcggcg ctgggcgcgc acgaccacgt cctgctgctc gggtgtcagc ggcccggcgt
     2881 agccctccgc
//
LOCUS       DAOSIF010000409         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_30002_length_2889_cov_2.725829, whole genome shotgun sequence.
ACCESSION   DAOSIF010000409 DAOSIF010000000
VERSION     DAOSIF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30002_length_2889_cov_2.725829"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..546)
                     /locus_tag="PLT35_11795"
     CDS             complement(43..546)
                     /locus_tag="PLT35_11795"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HOG29952.1"
                     /translation="MVRILITGGTFDKRYDEIRGRLHFTESQVPDMLKLGRSRVEVTL
                     RTVMLVDSLEMTPADRALIADNCAQAPEPRLVVTHGTDTMVETARAIAERVADKTVVL
                     TGAMVPYAFGSSDGLFNLGSALSFAQVLPPGVYIAMNGRYFSWDRVRKNRETGVFEEV
                     EGPGAGR"
     gene            676..>2889
                     /gene="metH"
                     /locus_tag="PLT35_11800"
     CDS             676..>2889
                     /gene="metH"
                     /locus_tag="PLT35_11800"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOG29953.1"
                     /translation="MAAYDSIIAGLDAILRERILVLDGAMGTMVQRRGLDEAAFRGER
                     FAAHPTDLKGDNDLLALTRPDVIRAIHDAYLEAGADIIETDTFNGTSIAQADYGMEPF
                     VYEMNAAAARIAREAAREWTARTPGRPRFVAGSMGPTSKTLSLSPDVSNPAFRAVTFE
                     QVKAAYAEQARGLLDGGCDLLLVETIFDTLNAKAALAAIDELFEARGARAPVLISVTV
                     ADRSGRTLSGQDVEAFWVSVAHARPYSVGINCALGAREMRPFLADLARVAGNTRVSCY
                     PNAGLPNAFGEYDEAPAETAALLREFADAGLVDIVGGCCGTTPDHIRAIAGAVAGVRS
                     RAARRAASGAAPGAACSQYAGLEAYAIRPDGTFTVIGERTNVSGSARFRRLIRDGQFA
                     AAAEVALDQVRGGANILDVNMDEGLLDSERAMREFLAFIASEPAVARVPVMIDSSKWS
                     VIEAGLQSVQGKALVNSISLKEGEADFLRKARLIRRYGAAAVVMAFDERGQADTVDRR
                     VQILQRAYGLLTRDAGFDPLDVVLDPNVLAIGTGLEEHADYAVDFIEAVRILKATCPG
                     AKTSGGISNLSFAFRGNDAVREAMHAAFLYHAIQAGLDMGIVNAGQLAVYEDIEPGLR
                     ERVEDLIFNRRADATERLVQYAATISGRVERRAADLTWRESPVDSRLAHALVHGIEEY
                     IERDAEEARVALGRPLFVIEGPLMSGMRTVGDLFGAGKMFLPQVVKSARVMKRAVA"
BASE COUNT          404 a         1024 c         1039 g          422 t
ORIGIN      
        1 ctccttcccc tcgtcccctg cttgctcctg tctgaccagc gcctagcgcc cagcccctgg
       61 accctcgact tcctcgaaca cccccgtctc cctgttcttg cgcacgcggt cccaggagaa
      121 gtaccgcccg ttcatcgcga tgtagacgcc gggcggcagc acctgcgcga acgacagcgc
      181 gctgccgagg ttgaacagcc cgtcggagct gccgaaggcg tagggcacca tggcgccggt
      241 caggacgacg gtcttgtccg cgacgcgttc ggcaatcgcc cgcgcggtct cgaccatcgt
      301 gtcggtgccg tgggtgacga cgaggcgcgg ctcgggggcc tgggcgcagt tgtcggcgat
      361 gagcgcgcgg tccgccggcg tcatctcgag actgtccacg agcatgaccg tgcgcagcgt
      421 gacctccacg cgggagcgcc cgagcttcag catgtcgggc acctgcgact ccgtgaagtg
      481 aaggcgcccg cggatctcgt cgtagcgctt gtcgaaggtg ccaccggtga tgaggatcct
      541 gaccatgacg cggtattcta gcccgggttc gggccgcgcc gggcgccggt ggcgcccccg
      601 ccgctgccgt ccccctgcta tgatctccgg gtgatctctt cgccgtcgac gggaccggcg
      661 gcgccggagg caggcgtggc cgcatacgat tcgatcatcg ccgggctcga cgcgatcctg
      721 cgcgaacgca tcctcgtgct ggacggcgcc atgggcacga tggtgcagcg ccgcggcctc
      781 gacgaggcgg ccttccgcgg cgagcgcttc gcggcgcacc cgacggacct taagggcgac
      841 aacgacctgc tggcgctcac gcgcccggac gtcatccgcg ccatccacga cgcctatctc
      901 gaggccgggg ccgacatcat cgagaccgac acgttcaacg gcacctccat cgcgcaggcc
      961 gactacggga tggagccgtt cgtgtacgag atgaacgccg ccgctgcgcg gatcgcccgc
     1021 gaggccgccc gcgagtggac cgcgcggacg ccgggccggc cccggttcgt cgccggatcg
     1081 atgggcccga cgagcaagac gctctcgctg tcgccggatg tctcgaaccc cgcgttccgg
     1141 gcggtcacct tcgagcaggt gaaggcggcg tacgcggaac aggcgcgcgg cctgctcgac
     1201 ggcggctgcg acctgctgct ggtggagacg atcttcgaca cgctgaacgc gaaggcggcg
     1261 ctcgcggcca tcgacgagct gttcgaggcg cgcggcgcgc gggcgccggt cctgatttcg
     1321 gtgaccgtcg ccgaccgcag cggccggacg ctgtcgggac aggacgtcga ggcgttctgg
     1381 gtgtcggtgg cgcacgcgcg gccgtactcg gtcggcatca actgcgcgct gggcgcgagg
     1441 gagatgcgcc cgttcctggc ggatctcgcc cgcgtggccg ggaacacgcg cgtcagctgc
     1501 tatcccaacg ccgggctgcc gaacgcgttc ggcgagtacg acgaggcgcc cgccgagacg
     1561 gccgcgctcc tccgcgagtt cgcagacgcc ggactggtgg acatcgtcgg cggatgctgc
     1621 gggacgacgc ccgaccacat ccgcgccatc gccggcgcgg tcgccggcgt gcgctcgcgc
     1681 gccgcgcgcc gggccgcctc gggcgccgca cccggcgcgg cctgctccca gtacgccggc
     1741 ctcgaggcgt acgcgatccg gccggacggc accttcacgg tgatcggcga gcggacgaac
     1801 gtttcgggct cagcccggtt ccgccggctg atccgcgacg ggcagttcgc cgccgcggcc
     1861 gaggtggcgc tcgaccaggt tcggggcggg gccaacatcc tcgacgtgaa catggacgag
     1921 ggcctgctcg actcggagcg ggcgatgcgg gagttcctcg ccttcatcgc ctcggagccg
     1981 gccgtcgccc gcgtgcccgt gatgatcgac agctcgaagt ggtcggtgat cgaggccgga
     2041 ctgcagtcgg tccagggcaa ggccctggtg aactcgatca gcctgaagga gggcgaagcc
     2101 gacttcctcc ggaaggcgcg cctcattcgc cgctacggcg ccgccgccgt cgtcatggcc
     2161 ttcgacgagc gcgggcaggc ggacacggtc gaccgccggg tccagatcct gcagcgcgcc
     2221 tacggcctgc tcacccgcga cgccggcttc gatcccctcg atgtcgtcct ggatccgaac
     2281 gtgctggcca tcgggaccgg cctcgaggag cacgcggact acgccgtgga cttcatcgag
     2341 gccgtccgga tcctcaaggc cacgtgcccc ggcgcgaaga cgagcggagg catctcgaac
     2401 ctgtcgttcg cgttccgggg caacgatgcc gtgcgcgagg cgatgcacgc ggcgttcctg
     2461 tatcacgcga tccaggcagg cctggacatg ggcatcgtca acgccgggca gctggcggtg
     2521 tacgaggaca tcgagcccgg ccttcgcgag cgcgtcgagg acctcatctt caaccgccgg
     2581 gcggacgcga ccgagcgcct ggtgcagtac gcggcgacga tttccggcag ggtggagcgc
     2641 cgggccgccg acctcacctg gcgggagtcg cccgtcgact cccgcctggc gcacgccctt
     2701 gtccacggca tcgaggagta catcgagcgg gatgccgagg aggcccgcgt ggcgctgggc
     2761 cggccgctct tcgtcatcga agggccgctg atgagcggca tgcggacggt cggcgacctc
     2821 ttcggcgcgg gcaagatgtt cctgccgcag gtggtgaaga gcgcgcgggt catgaaacgc
     2881 gcggtggcg
//
LOCUS       DAOSIF010000410         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_30081_length_2883_cov_3.283946, whole genome shotgun sequence.
ACCESSION   DAOSIF010000410 DAOSIF010000000
VERSION     DAOSIF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30081_length_2883_cov_3.283946"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>298)
                     /locus_tag="PLT35_11805"
     CDS             complement(<1..>298)
                     /locus_tag="PLT35_11805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG29954.1"
                     /translation="HYAQIDYRKMIAWPQRLAREWAFLSGPFASLPSRRVLDLGCGTG
                     EHVRFLAGHGYEVVGVDESETMLDRAREAGVPPGASYLQGDLVRVDSVVSGEFGA"
     assembly_gap    299..308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            680..1303
                     /gene="udk"
                     /locus_tag="PLT35_11810"
     CDS             680..1303
                     /gene="udk"
                     /locus_tag="PLT35_11810"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOG29955.1"
                     /translation="MAADPVIIGVAGGSGSGKTTVVRRIVESLGNDQVTVLEHDRYYR
                     DRGDLRLEERAALNYDHPDSLETDLMVRHLRALRRGSAVDVPVYDFARYARTAATERV
                     SARRAIIVEGILIYTDADLRSLLDVKVFVDADDDIRFIRRLQRDVAERGRTMASVIDQ
                     YLSTVKPMHLDFVEPSKRYADIIVPQGGHNTVAIGMLLTLIRSLAQA"
     gene            1340..2215
                     /locus_tag="PLT35_11815"
     CDS             1340..2215
                     /locus_tag="PLT35_11815"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOG29956.1"
                     /translation="MRWQRGTSDRDIEDRRGRPSGGFGGVGGGRMRIGLGGLVVIGLL
                     SLLFGQDFFSLLGGLPADPGTSVAVPPDRPVNQSAEEAELYEFVKFVVNDVQDTWTKL
                     LPQQAGAQYVRSTLVLYRDAVRSACGFADASSGPFYCPGDRKVYIDLGFYDELRARFG
                     ASGDFAQAYVIAHEFGHHIQNVLGIDEQTRRAQRRRPDLQNELSVRLELQADCLAGVW
                     GHSTAERKLLESGDVEEGLNAAAAIGDDRIQRMSGRAVQRDAFTHGSSAQRVAWFRRG
                     LESGRFASCDTFGSQ"
     gene            2229..2561
                     /locus_tag="PLT35_11820"
     CDS             2229..2561
                     /locus_tag="PLT35_11820"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOG29957.1"
                     /translation="MLVVHVHVRVRPDAIDAFRDATVVNATESLKEPGIARFDVLQQE
                     DDPTRFILVEAYRTAGAVSAHKETAHYAAWRDAVAPLMAEPRRGTRFVTVVPDDSRYG
                     TPAGSRTE"
     gene            2558..>2883
                     /locus_tag="PLT35_11825"
     CDS             2558..>2883
                     /locus_tag="PLT35_11825"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG29958.1"
                     /translation="MTAFEFATAGRVIFGDGASAGVPELAAGWGARVLVVAASDPARA
                     EWLVTGLEQRGAEVALLCVEDEPDTSFVEGGAGLARRRGIEAVVGLGGGSALDAAKAI
                     AALATNP"
BASE COUNT          408 a         1017 c         1066 g          382 t
ORIGIN      
        1 ccgcgccgaa ctcgcccgac accaccgagt ccacgcgcac caggtcgccc tgcaggtagc
       61 tcgcgcccgg gggcacgccg gcctcccgcg cgcgatcgag catggtctcg gactcgtcga
      121 cgcccaccac ctcgtagccg tgcccggcga ggaaccgcac gtgctcgccc gtgccgcatc
      181 caagatccag cacccgcctc gagggcagcg aggcgaacgg ccccgacagg aacgcccact
      241 cccgcgcgag cctctgcggc cacgcaatca tcttccggta gtcgatctgc gcgtagtgnn
      301 nnnnnnnncg aactcatcga cgccccccct gcttccgact ctccggccgg cggcgcaccg
      361 gcgtcaacct cgcccccgtg ccggtctcag gcccgctgca gtcgcggccg aagcgcggcg
      421 cctgccgccg cgacccggca gagcggggcc ggagcctccg ccgcgctttc cgtttgacgg
      481 gggcgaggcc gctgcgtaag gtgggaagcg ggcgctccgg gccttgtccg ctcgagaggc
      541 gctcggagat cggcacgccg ggcgagggca acacgccgcg cttcctgcgg cgcccgctcg
      601 ggaagagcga caaggcgctt caggagcccg cggcgcggcc gctcgccgcg tgagtcctgg
      661 gcggaagggg gcgcagcaga tggcagccga cccggtgatc atcggcgtcg cgggcggttc
      721 ggggtcgggc aagacgacgg tggtccggcg gatcgtggag agcctcggca acgaccaggt
      781 gacggtgctc gagcacgacc gctactaccg ggaccgcggc gacctgcgcc tcgaggagcg
      841 ggccgcgctg aactacgacc accccgactc gctggagacg gacctgatgg tgcgccacct
      901 ccgcgcgctg cggcggggat cggcggtgga cgtgccggtg tacgatttcg ccaggtacgc
      961 gcgcacggcg gccacggaga gggtctccgc cagacgcgcc atcatcgtcg agggcatcct
     1021 gatctacacc gacgcggacc tgcgctcgct gctcgacgtg aaggtgttcg tggacgccga
     1081 cgacgacatc cggttcatcc ggcggctcca gcgggacgtc gccgagcgcg gccggacgat
     1141 ggcatcggtc atcgaccagt acctgagcac ggtcaagccc atgcacctcg acttcgtcga
     1201 gccgagcaag cgatacgcgg acatcatcgt gccgcagggc ggccacaaca ccgtggccat
     1261 cggcatgctc ctgaccctca tccgcagcct ggcccaggca tagggccgga ggggcgcgcg
     1321 gccctcgagg agaaccgtca tgaggtggca gcggggcacg agcgaccggg acatcgagga
     1381 ccgacgggga cggccgtccg gcgggttcgg cggcgtcggc ggcgggcgca tgagaatcgg
     1441 gctgggcggc ctggtcgtca tcggcctgtt gagcctgctc ttcgggcagg acttcttctc
     1501 gctcctcggc ggccttccgg cggacccggg aacgtccgtg gcggtgccgc cggaccggcc
     1561 ggtgaaccag tcggcggaag aagccgagtt gtacgagttc gtgaagttcg tcgtgaacga
     1621 cgtccaggac acctggacga agctgctgcc gcagcaagca ggcgcccagt acgtgcggtc
     1681 gacgctcgtg ctctaccgcg acgccgtccg gtccgcgtgc gggttcgccg acgcgtcgtc
     1741 cgggccgttc tactgcccgg gggaccgcaa ggtctacatc gacctcggct tctacgacga
     1801 gctgcgggcg cggttcgggg cctcgggcga tttcgcccag gcctacgtga tcgcgcacga
     1861 gttcgggcat cacatccaga acgtcctcgg catcgacgag cagacgaggc gggcccagcg
     1921 gcggcggccc gacctgcaga acgagctctc ggtgcgcctc gagctgcagg cggactgcct
     1981 cgccggcgtg tgggggcact cgacggcgga gcggaagctg ctcgagtcgg gcgatgtgga
     2041 ggaagggctg aacgccgccg cggcgatcgg cgacgatcgc atccagcgca tgtccggccg
     2101 ggccgtccag cgggacgcgt tcacgcacgg ctcctcggcg cagcgcgtgg cgtggttccg
     2161 ccgcggcctg gaatcggggc gcttcgcctc gtgcgacacc tttggcagcc agtgatcgga
     2221 gggccgacat gctcgtcgtc cacgtgcacg tgcgcgtgcg gccggacgcc atcgacgcgt
     2281 tccgcgacgc gaccgtcgtc aacgcgaccg agagcctgaa ggagcccggc atcgcccggt
     2341 tcgacgtgct ccagcaggaa gacgacccga cccggttcat cctcgtcgag gcctaccgca
     2401 ccgccggtgc cgtgtccgcc cacaaggaaa ccgcgcacta cgccgcgtgg cgcgacgcgg
     2461 tggcgccgct gatggcggag ccgaggcgcg gcacgcggtt cgtcaccgtc gtgcccgacg
     2521 acagccggta cggcacgccg gccgggagcc ggaccgagtg acggcgttcg agttcgcgac
     2581 ggccggccgc gtgatcttcg gcgacggcgc ttcagcgggc gtgccggagc tggcggcggg
     2641 atggggcgcg cgggtgctcg tcgtcgctgc ttccgatccg gcgcgcgccg agtggctcgt
     2701 cacgggcctg gagcagcgcg gcgccgaggt ggccctgctg tgcgtcgagg acgagccgga
     2761 cacctcgttt gtcgagggcg gggccgggct ggcccggcgg cgcggcatcg aggcggtcgt
     2821 cgggctcggc ggcgggagcg cgctggacgc cgccaaggcg attgccgcgc tcgccacgaa
     2881 ccc
//
LOCUS       DAOSIF010000411         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_30125_length_2879_cov_3.399788, whole genome shotgun sequence.
ACCESSION   DAOSIF010000411 DAOSIF010000000
VERSION     DAOSIF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30125_length_2879_cov_3.399788"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLT35_11830"
     CDS             <1..571
                     /locus_tag="PLT35_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG29959.1"
                     /translation="GTIRVAAWPGWAPPRKRLAHGVGLLSEDRKGEGLALAMPLADNA
                     TLSRLEGLGPAGFVLPRRQHAAAARWIDRLGIKCRDSRQRVGELSGGNQQKVALARLL
                     HHDVDVFLLDEPTRGIDVGSKAEIYRLIDALALAGKAVLLVTSSLPELLGVCDRVAIM
                     HRGRLGPLRAPQGLTEHAVLMEAMGVAGG"
     gene            719..1642
                     /locus_tag="PLT35_11835"
     CDS             719..1642
                     /locus_tag="PLT35_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG29960.1"
                     /translation="MSTVVVRNPEERRLNQRAMRLSLAVGFAMLAGKAAAYVLTGSSA
                     IMSDALESVIHVLAVAFAAFSLSLSAKPADSRFPYGYERIAFFSAGFEGALIILAAAS
                     IIWTAVARWLHGLQLERLGTGVLLVLAASVVNGALGWHLVRTGRRSKSIILEANGRHV
                     LTDCWTSLGVVVGLLLVMATGWLPFDPIVAIAVAVNILWSGAHLVFRSVSGLMDYADP
                     ARTARVTAAVEPFSRLLGFHHHGLRVRHTGNRTLVEIHLLFPFSLTLGDAHRRATVLE
                     AHVANALGEEVEVITHLESLEDHAAVHGPEH"
     gene            complement(2056..>2879)
                     /locus_tag="PLT35_11840"
     CDS             complement(2056..>2879)
                     /locus_tag="PLT35_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG29961.1"
                     /translation="EDVERRRRVAFLGTDVARKLFGNSPAVGQTVRIKGLSFEVVGVL
                     TDKAQLSSYFWPDRMSVFIPHSVTEQLFHQDYLDTIVVQTIAPAFHERGMRQVRAVLA
                     ERHRFDARDERALSVHDSAEMRRIFGGMTMGLKLVLVFIGTLTLMIGGVGVMNIMLVS
                     VTERTREIGVRKALGARRRHILTQFLLEGLVITGLGGAVGIALTAILVSVSGRRPFLA
                     RLIGDPTGETDIHLLLTPDVLLATTLVLMLTGVLSGLWPALRASRLDPIESLRYE"
BASE COUNT          411 a         1020 c         1002 g          446 t
ORIGIN      
        1 cggtacgatc cgagtggcgg cctggccggg atgggcgccc ccgcgcaagc ggctcgcgca
       61 cggcgtgggc ctcctcagcg aggatcgcaa gggagaaggc ctagcgctcg ccatgccgct
      121 cgccgacaac gccacgctct cgcgcctcga gggcctcggg ccggcggggt tcgtgcttcc
      181 ccgccgtcag cacgccgccg ccgcgcgctg gatcgatcgg ctcggcatca agtgccggga
      241 cagccgccag cgtgtcggcg agctctccgg cggcaaccag cagaaggtgg cgctcgcgcg
      301 gctgcttcac cacgacgtgg acgtgttcct cctcgacgag cccacccgcg gcatcgacgt
      361 cgggagcaag gccgagatct accgcctcat cgacgccctc gcgctggcgg gcaaggccgt
      421 cctgctcgtg acgagttctc tgccggaact gctcggggtg tgcgaccggg tggctatcat
      481 gcacagaggg cgcctcggcc cgctcagggc gccgcagggg ctcaccgagc acgcggtcct
      541 catggaggcg atgggagtgg caggaggcta ggccaggttc ggggctcggg acgcgagctt
      601 caggactcgc gcgttctgcg cggaaaccgg tccttcggct cgctcatggc gggccggcgg
      661 gccgcatggc ggaggcgggg gctcgggagc tggcaggcgg gagccgaggg aaggttcgat
      721 gagcacggtt gttgtgcgca atccggagga gcggaggctg aaccagcgcg ccatgcggct
      781 gtcgctggcc gtcggcttcg ccatgctcgc cggcaaggcc gccgcgtacg tcctcacggg
      841 atcgtcggcc atcatgtccg acgccctcga gtcggtcatc cacgtcctgg ccgtcgcctt
      901 cgccgccttc agcctctcgc tcagcgccaa gccggccgat tccaggttcc cctacggcta
      961 cgagcgcatc gccttcttct cggcggggtt cgaaggcgcg ctgatcatcc tggcggcggc
     1021 gtcaatcatc tggacggccg tggccaggtg gctgcacggc ctgcagctcg agcggctcgg
     1081 cacaggcgtc ctgctcgtcc ttgccgcctc tgtcgtgaac ggcgcgctgg gctggcacct
     1141 ggtgcgcacg gggcgccggt cgaagtcgat catcctcgag gccaacggcc gccacgtcct
     1201 taccgactgc tggacgagcc tgggcgtggt cgtcgggctc ctgctggtga tggcgacggg
     1261 ctggctgccc ttcgacccga tcgtcgccat cgcggtcgcc gtgaacatcc tgtggtccgg
     1321 cgcgcacctc gtgttccggt cggtgagcgg cttgatggat tacgccgacc ccgcgcggac
     1381 cgcgcgcgtc acggccgccg tcgagccgtt cagccggctt cttggcttcc atcaccatgg
     1441 cctccgggtc cgccacacgg gcaaccggac cctcgtcgag atccacctgc tctttccgtt
     1501 ctccctgacg ctcggagacg cccatcgccg cgcgacggtg ctcgaagcgc atgtggcgaa
     1561 cgcgctcggc gaggaggtgg aagtcatcac gcacctcgaa tcgctcgagg accacgccgc
     1621 ggtgcacggg cctgagcact aacggcacga accgccctgc ctgagccttg tggcccccgg
     1681 tccgggccgc cgggtgccat tttctgtcgt gccggtgacg ctccgtgtcg aagagcgtca
     1741 gcacgccggc gcgcggccgg tggcccgtga tgttcaaacc cttatgttga aggcacttgc
     1801 gagcctgcgc gaagctcgcg agaccgtggc aggacgcttg caattcgctc gggtgcgagg
     1861 aatgcgaaag cactccagac cacagcctgc ggcgtggtgg cctccacacc gccggctcgc
     1921 ggagaggggg gtctgacccg cccgcctctc ccacgccagt ctcctctcca accgccgtag
     1981 ccggggggct cgagccctcc ggctacacct ctgtgggggg cagatccgga aactcgcgga
     2041 tctgaccccg tttcctcatt cgtagcgcag ggactcgatc ggatcgaggc gcgaggcgcg
     2101 cagcgccggc cagaggccgc tgaggacccc ggtcagcatc aggacgagcg tcgtcgccag
     2161 gagcacgtcc ggcgtgagga ggaggtggat gtccgtctcg ccggtcgggt cgccgatgag
     2221 gcgggcaaga aacggccggc ggccggagac gctcaccagg atcgccgtga gggcgatgcc
     2281 gacggccccg cccaggccgg tgatgaccag cccctcgagc aggaactgcg tcaggatgtg
     2341 gcgccggcgc gcgcccagcg ccttccgcac gccgatctcc cgcgtccgct ccgtcaccga
     2401 caccagcatg atgttcatga cgcccacgcc gccgatcatc agcgtcaagg tgccgatgaa
     2461 cacgagcacg agcttcagcc ccatcgtcat gccgccgaag atccggcgca tctcggcgct
     2521 gtcgtgcacc gacagcgcgc gctcgtcgcg cgcgtcgaag cggtgccgct cggccagcac
     2581 cgcgcggact tgccgcatcc cccgctcgtg gaacgccggc gcgatggtct gcacgacgat
     2641 ggtgtcgagg tagtcctggt ggaagagctg ctcggtcacc gagtgcggga tgaacacgct
     2701 catccggtcc ggccagaagt agctcgacag ctgggccttg tcggtgagca cgccgacgac
     2761 ctcgaacgag agccccttga tccgcaccgt ctggccgacg gccgggctgt tgccgaagag
     2821 cttcctcgcg acgtcggtgc cgagaaacgc gacccgccgc cgccgctcga cgtcctcgg
//
LOCUS       DAOSIF010000412         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_30380_length_2861_cov_3.355666, whole genome shotgun sequence.
ACCESSION   DAOSIF010000412 DAOSIF010000000
VERSION     DAOSIF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30380_length_2861_cov_3.355666"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLT35_11845"
     CDS             <1..855
                     /locus_tag="PLT35_11845"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29962.1"
                     /translation="VYRDLAERMAPFPVTIRTFDVDESHGVHEGGRIGADPDLERGEA
                     ASRGPLGLRAIRLSLNRRDAFETQLRALSRAACHGSLRVLLPFVSGVEEVREAKSVLE
                     GAWRAACRGIAGAPPRVPVGAMIEVPSAALTVDLLAAEADFFSIGTNDLIQYCLAVDR
                     TDGRVARLFDPLHPAILRVIRHVARLARRRRRTVAVCGEMASDPRALLLLLGLGVTEF
                     SMRPSAIPTARHLVRRVAMADAQRASARALGLATSGEIAAMLAEAFPGAVAGPGGEND
                     GPLEEDEP"
     gene            852..1280
                     /locus_tag="PLT35_11850"
     CDS             852..1280
                     /locus_tag="PLT35_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG29963.1"
                     /translation="MIEDARPAGARTEKPWGYELLWAKTSQYVGKIIHVNAGHALSLQ
                     YHRVKTESVYLAAGRVVYEIEEGGRLARRELVPGDHLHIPAGTVHRVTAIEDADIFEV
                     STPELDDVVRLEDRYGRARAPATTESGGRGPGEESGPPGA"
     gene            complement(1311..1736)
                     /gene="ssb"
                     /locus_tag="PLT35_11855"
     CDS             complement(1311..1736)
                     /gene="ssb"
                     /locus_tag="PLT35_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG29964.1"
                     /translation="MANSYNKIILVGNLGRDAELRATPQGRQVLSSSLAVEDPSKKDD
                     RGRPGTEWYRIKVWGRQAETLSPLLLKGKSILVEGRLSIQTWTDREGHNRYTPEVSVD
                     RVVLLGSPGGGQAAQAREAHAVDDLDQTPADINADDIPF"
     gene            complement(1783..2628)
                     /gene="ybgF"
                     /locus_tag="PLT35_11860"
     CDS             complement(1783..2628)
                     /gene="ybgF"
                     /locus_tag="PLT35_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOG29965.1"
                     /translation="MMRRPGPFVFALGLLLAAPALAPIDAASKEQQQMMADIRMLQQQ
                     NQRLQAQLAAVTDLLKAVTARLDEQGAGTRKGFADQKVLTDTMGADLRVVREKIDETN
                     VRLRSLLEEVETLRAAQNAALAAAPAPPSEPDAAPAGGPPAQPAPRPGSFGASPSQAF
                     ESARSDYYMGQWSLAIQGFESYLKTFPRSDLADDAQYYIGETYYMSGRFQDAVAAYNL
                     VIERYPGSNTLPDAYYKRGLALATLGQVDAARESLSFAVATYPDSDAGRLAKQALDKL
                     SRAGK"
     gene            complement(2649..>2861)
                     /locus_tag="PLT35_11865"
     CDS             complement(2649..>2861)
                     /locus_tag="PLT35_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG29966.1"
                     /translation="GHCDERGTAEYNLALGERRAVAAQAYLTSLGIAADRLKTVSYGK
                     EFPFDPGHDEAAYAKNRRAHFVITGK"
BASE COUNT          382 a         1010 c         1020 g          449 t
ORIGIN      
        1 gtctatcgcg atctcgccga gcgcatggcg ccgtttcccg tgacgatccg cacgttcgac
       61 gtggacgagt cccacggggt gcacgaaggc ggccggatcg gcgccgaccc cgatctcgag
      121 cggggcgagg cggcgtcgag gggcccgctc ggcctgcggg cgatccggct gagcctgaac
      181 aggcgcgacg ccttcgagac ccagctgcgc gcgctctcca gggccgcctg ccacgggtcg
      241 ctgcgggtgc tgctgccctt cgtgtccggc gtggaggagg tgcgggaggc gaagtcggtc
      301 ctcgagggcg cctggcgggc cgcctgccgc gggatcgcgg gtgcgccacc gcgggttccg
      361 gtcggcgcga tgatcgaagt gccctcggcc gccctgacgg tggacctgct cgccgcagag
      421 gccgacttct tcagcatcgg cacgaacgat ctcatccagt actgcctggc cgtcgatcgc
      481 accgacgggc gcgtcgcgcg cctgttcgat cccctgcacc cggccatcct gcgcgtgatc
      541 aggcacgtgg cccggctggc gcggcgccgg cggcgcaccg tcgcggtgtg cggggagatg
      601 gcctccgatc cccgggcgct gctgctcctg ctcggcctcg gcgtcaccga gttcagcatg
      661 aggccgtcgg ccattccgac agcccgtcac ctcgtgaggc gcgtggcgat ggccgacgcg
      721 cagcgggcgt ccgcgcgagc gctcggcctc gcgacgtccg gcgagatcgc cgcgatgctc
      781 gccgaggcct tcccgggcgc cgttgccggg ccgggcggcg agaacgacgg gccgctggag
      841 gaggatgagc catgatcgaa gatgccaggc ccgcgggcgc gcgcaccgaa aagccttggg
      901 gctacgaact gctgtgggcc aagaccagcc agtacgtcgg caagatcatc cacgtcaacg
      961 ccggccacgc gctgagcctg cagtaccacc gcgtcaagac ggagagcgtg tacctggcgg
     1021 cgggacgggt cgtctacgag atcgaggaag gcggacgcct ggcgcgtcgc gaactggttc
     1081 cgggagacca cctgcacatt cccgccggaa cggtccaccg cgtcacggcc atcgaggacg
     1141 cggacatctt cgaagtgtcc acgcccgagc tcgacgacgt cgtgcgcctc gaggatcgat
     1201 acggaagggc gagggcgccg gcaacgacgg aatccggagg ccgcgggccg ggagaggaga
     1261 gtggcccccc cggcgcctga ctcccgcgtc cggactccgt ccgatccgcc ctagaacgga
     1321 atgtcgtccg cgttgatgtc ggccggcgtc tgatcgaggt cgtctacggc gtgcgcctcg
     1381 cgcgcctgag cggcctgtcc gccgccgggg ctgccgagca ggacaacccg atccaccgag
     1441 acctccggcg tgtatcggtt gtgcccctcg cggtcggtcc aggtctggat ggagagccgg
     1501 ccctcgacca ggatgctctt gcccttcagc aggagcgggc tcagggtctc cgcctgacgg
     1561 ccccacacct tgatccggta ccactccgtg ccgggccggc cgcggtcgtc cttcttcgac
     1621 gggtcctcga cggcgaggct ggaggacagc acctgccggc cctgcggcgt cgcgcgcagc
     1681 tcggcgtcgc gcccgaggtt gcccacgagg atgatcttgt tgtagctgtt cgccatgctg
     1741 gtcctttcga cgggcccgtc gccggcgccc cgaatcgcag cgctatttgc cggcgcgcga
     1801 gagcttgtcc agggcttgtt tcgccagccg tcccgcatcg ctgtccgggt aggtcgcaac
     1861 ggcgaacgag agcgactccc tggccgcgtc gacttgcccc agcgtcgcca gcgccaagcc
     1921 acgcttgtag taggcgtccg gcagcgtgtt gctcccgggg tagcgttcga tgacgaggtt
     1981 gtaggccgcc accgcgtcct ggaagcgtcc cgacatgtag tacgtctcgc cgatgtagta
     2041 ctgcgcgtca tcggcgagat cggacctcgg aaacgtcttg aggtagctct cgaagccctg
     2101 gatcgccagg ctccactgcc ccatgtaata gtccgaccgc gcggactcga aggcctgcga
     2161 gggcgaggcg ccgaagcttc ccgggcgcgg cgcgggctgg gcgggagggc cgccagccgg
     2221 cgccgcgtcg ggttcggacg ggggcgccgg agcggcggcg agcgcggcgt tctgggccgc
     2281 ccgcagcgtc tcgacctctt cgagcagcga ccgcagccgg acgttcgtct cgtcgatttt
     2341 ctcgcgtacg acgcgcagat cagcgcccat cgtgtcggtc aggaccttct ggtccgcgaa
     2401 gcccttccgc gtgccggcgc cctgctcgtc gaggcgcgcc gtcaccgcct tcagcaggtc
     2461 cgtgacggcg gccagctggg cctgcaggcg ctggttctgc tgctgcagca tccgaatgtc
     2521 ggccatcatc tgctgctgct ccttgctggc cgcgtcaatc ggcgccagcg ccggcgccgc
     2581 cagcaggagc cccagggcga acacgaacgg tccgggtcgt cgcatcatgc ctcgcctcgc
     2641 ttcccggcct atttcccggt gatcacgaag tgcgcgcggc ggttcttcgc gtaggccgct
     2701 tcgtcgtggc ccggatcgaa cgggaactcc ttgccgtaac tgaccgtctt caggcggtcc
     2761 gccgcgatgc cgagcgaggt gagataggcc tgggcggcca cggcccggcg ctcaccgagg
     2821 gccaggttgt actccgccgt gccgcgctcg tcgcagtgcc c
//
LOCUS       DAOSIF010000413         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_30533_length_2850_cov_3.139893, whole genome shotgun sequence.
ACCESSION   DAOSIF010000413 DAOSIF010000000
VERSION     DAOSIF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30533_length_2850_cov_3.139893"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PLT35_11870"
     CDS             <1..1584
                     /locus_tag="PLT35_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29967.1"
                     /translation="MGAGKFDGAHLVANFEAGLAQSYNYNFSNTSNPKQAADFPVYQF
                     GFYAGDKWRARSNFTLTYGVRVDIPRFSEKPNANPVAVANFGYKTDNVPAPVMWSPRV
                     GFNWDLSKSGARRQVRGGVGVFTGRTPYVWLSNQYSNTGVDFTSLSVSYNAANRLPFV
                     ANPNGQPSVVVGGTTGRQTINVVDPNYKYPEILRGTFGVDHELPWGLVGTAEGVYTRS
                     FHDIFYQNINYVPAGALPDGRTTFKKFDTSLNDVMLLSNSTDGKSWSVSYKIERPYRK
                     GFTWSASYLYGRAYARNDGTSSVARSNWTNNPSGLNTNDPPLTRSNYDPGHRVNLSAT
                     LPIPLFKGLRSSLAFFFNGQSGRPFSIGFNGDANTDSITSNDLLFVPGSADDVVLYSS
                     VAGQTATWDILNNFLQKAEVGDYRGKIIARNHARAPWYNHLDVRYAVTVPLPRKARIE
                     LTADVFNFLNLLNKDWGWQYFGNFGSTNLIGYGGIDAATGKMRYNLSTLMGPNYSGLF
                     TRADLRSRWQAQFGARVRF"
     gene            complement(1693..1926)
                     /locus_tag="PLT35_11875"
     CDS             complement(1693..1926)
                     /locus_tag="PLT35_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29968.1"
                     /translation="MTVGKETAMGLVRLLGFSTLSCGCVVGRYRELATNRELAYIEEK
                     GGECPVHEHRRNHAVQAPRAAASASRFTHARAS"
     gene            2176..2490
                     /locus_tag="PLT35_11880"
     CDS             2176..2490
                     /locus_tag="PLT35_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOG29969.1"
                     /translation="MPTLRNKLYTIPSCPHCPVARAYLLSKGVDFVEFDVSTDVEALR
                     AMLTMTARPEVPTIVAGDRAVVGFNPQSWDALLERSAELQREDPYEIPESLGADPYRG
                     VD"
     gene            2625..>2850
                     /locus_tag="PLT35_11885"
     CDS             2625..>2850
                     /locus_tag="PLT35_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29970.1"
                     /translation="MERLKRAAAALGVFLMLAPVGPVSAQTAARKAPARPPEKPASSA
                     AGKPAPASKAPAAATATARTASKAGGTATAP"
BASE COUNT          480 a          955 c          963 g          452 t
ORIGIN      
        1 atgggcgcgg gcaagttcga cggcgcccac ctggtcgcga acttcgaggc gggcctggcg
       61 cagtcctaca actacaactt ctcgaacacc tcgaacccga agcaggccgc cgacttcccg
      121 gtgtaccagt tcgggttcta cgcgggcgac aagtggcggg cgcggagcaa tttcacgctg
      181 acctacggcg tccgcgtgga catcccgcgt ttctcggaaa agcccaacgc gaatcccgtc
      241 gcggtggcga acttcggcta caagaccgac aacgtcccgg cgccggtgat gtggtcgccg
      301 cgcgtcgggt tcaactggga tctctccaag agcggcgcgc ggcgccaggt ccgcggcggc
      361 gtgggcgtgt tcaccgggcg gaccccctac gtgtggctct cgaaccagta cagcaacacg
      421 ggggtcgact tcacgagcct gtcggtcagc tacaacgcgg caaaccggct gccgttcgtc
      481 gccaatccca acgggcagcc gtcggtcgtc gtcggtggca cgacgggacg ccagacgatc
      541 aacgtcgtcg acccgaacta caagtacccg gagatcctgc gcgggacgtt cggggtcgac
      601 catgaactgc cctgggggct cgtgggcacg gcggaaggag tctacacgag gtcgttccac
      661 gacatcttct accagaacat caactacgtg ccggccgggg cgctgccgga cggccgcacg
      721 acgttcaaga agttcgacac gagcctgaac gacgtcatgc tgctgtcgaa ctcgacggac
      781 gggaagtcct ggtccgtcag ctacaagatc gagcggccgt accggaaggg gttcacgtgg
      841 agcgcctcgt acctctacgg gcgtgcctac gccagaaacg acggcacgtc gagcgtggcc
      901 cgatccaact ggacgaacaa cccctcgggg ctcaacacca acgatccgcc gctgacgcga
      961 tcgaactacg atccgggcca ccgcgtgaac ctgtcggcca cgctgccgat tcctctgttc
     1021 aaggggctcc gcagttcgct ggcgttcttc ttcaacggcc agtccggccg gccgttctcg
     1081 atcgggttca acggcgacgc caacacggac tcgatcacga gcaacgacct gctgttcgtc
     1141 ccggggtcgg cagacgacgt cgtgctgtac agctccgtgg cgggtcagac cgcgacgtgg
     1201 gacatcctga acaacttcct gcaaaaagcg gaagtcggcg actaccgcgg gaagatcatt
     1261 gcccgcaacc acgcccgggc gccctggtac aaccatctgg acgtgcggta cgccgtcacg
     1321 gtgccgctcc cgcggaaggc gaggatcgaa ctgacggctg acgtcttcaa cttcctcaac
     1381 ctgctgaaca aggactgggg ctggcagtac ttcggcaatt tcggctccac caacctgatc
     1441 ggctacggcg gcattgacgc cgccacgggc aagatgcggt acaacctctc gacgctcatg
     1501 gggccgaact actcggggct gttcacccgc gccgacctcc ggtcgcgctg gcaggctcag
     1561 ttcggcgccc gggtccgctt ctaggcaccg ggcgcgtcga agccgggggc tgtcgccccc
     1621 cggcctcgcg tacatgaacg ggggaggcgc gccttggggc gcctcccccg tgttccgtac
     1681 ggcgtggcgg ggtcaggatg cgcgcgcgtg agtgaaccgg gacgcggatg ccgccgcgcg
     1741 aggggcctgc acggcgtggt tcctccggtg ctcgtgcacg gggcactccc cgcccttttc
     1801 ctcgatgtag gcgagttcgc ggttcgtggc cagttcgcga taacggccga cgacacagcc
     1861 gcaggaaagc gtcgagaacc cgagcagtcg gacgagcccc atcgcggtct ccttgccgac
     1921 agtcatgcaa tcttcgcgcc gactcagggc cgcccggcgg ggccagcccc gccgagcgga
     1981 cgcggcaggg cgttcgcgcc gaatcatccg aaatcgccgc ggttgcccgc ggattgcgcg
     2041 tggggaggct ggcccccggc cgaggcgtcg ggcgacgggc cgctttcgct gagcctgcgc
     2101 ggcgacggaa gttggcagca gttgacagcg ggcgtcaatc tcgcgacact gtggccgcgc
     2161 cttccgcggc ggcaggtgcc gacgttgcgg aacaagctct acacgatccc gtcgtgcccg
     2221 cactgcccgg tggcacgggc atacctcctg tcgaagggcg tcgacttcgt cgagttcgac
     2281 gtctcgaccg acgtggaagc cctgcgggcc atgctgacga tgacggcgag gccggaagtt
     2341 ccaaccatcg tcgccggtga tcgcgctgtc gtggggttca acccccagtc gtgggatgcc
     2401 ttgctcgagc ggtcggccga actgcagcgc gaagatccct acgagatccc cgagtcgctg
     2461 ggcgccgatc cctatcgagg cgtggactga ggcctgggcc tccgagcgcc gataacagac
     2521 gagggcgcgt tttctgttgg ctcgatcgta tcgatttgat agactccgat cccaagtgct
     2581 ttgtcgtctg gttgatacag gtgactgaca cgcggatggc gacgttggaa cggctcaagc
     2641 gtgcggcggc ggcgctgggc gtcttcctga tgctggcgcc ggtcgggccg gtgtctgcac
     2701 agacggccgc ccggaaggct cccgctaggc cgcccgagaa gccggcatcg agcgcggcgg
     2761 gaaagcccgc gccggcctcg aaggcgcctg ccgcagccac ggcgacggcg cggactgcgt
     2821 cgaaagccgg ggggacggcc acggccccgg
//
LOCUS       DAOSIF010000414         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_30622_length_2844_cov_4.632126, whole genome shotgun sequence.
ACCESSION   DAOSIF010000414 DAOSIF010000000
VERSION     DAOSIF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30622_length_2844_cov_4.632126"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1113
                     /locus_tag="PLT35_11890"
     CDS             274..1113
                     /locus_tag="PLT35_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOG29971.1"
                     /translation="MGNTLKTALLLGLMSGVLLVLGDYFGGRGGLVMAFGFAVLMNFA
                     SYWFSDKIVLRMYRATPVGPGHPLFQITERLAQRAGLPMPRVFVIPDPSPNAFATGRS
                     PSHAAVAATEGILRLLDEREIEGVIAHELAHVKHRDILVGSVAATLAAAIMMMARMAQ
                     FAAFFGGGRSDDREGGSNPVALLATVILAPVAAMLIQAAISRSREFGADAGGAAIAGT
                     PYGLVDALKKLEYASKRVPLEANPATAHMFIVKPFSVHGLLSLFSTHPPTEARIRALL
                     GQA"
     gene            complement(1137..>2844)
                     /locus_tag="PLT35_11895"
     CDS             complement(1137..>2844)
                     /locus_tag="PLT35_11895"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOG29972.1"
                     /translation="EVSTMAIDPRHDPSRVVRAPRGTALTCKSWLTEAPYRLIQNNLD
                     PEVAENPQALVVYGGIGRAARDWACFDAILDALTALEDDETLLVQSGKPVGVFRTHAD
                     APRVLIANSNLVPRWATWDHFHELDRKGLFMYGQMTAGSWIYIGSQGIVQGTYETFAE
                     AGRQHYGGDWAGRWILTAGLGGMGGAQPLAATFAGAASLIVECDPARIAARLRTKYLD
                     VQAADLDDALARIRRHCARREAVSVGLLGNAAEVLPELVRRARAGGPVPGLVTDQTSA
                     HDLVNGYLPAGWTLERWAAARATPAEHEALRAAAGRSCAVHVRAMLDFKAMGVPVVDY
                     GNNIRQAAKDHGLDDAFAFPGFVPAYVRPLFCEGKGPFRWVALSGDPGDIHRTDEALK
                     ALFPDNAGVRRWLDMAARRISFQGLPSRICWLGLGERHLAGLAFNDMVRSGALKAPVV
                     IGRDHLDAGSVASPNRETEGMADGSDAVSDWPILNALLNTASGATWVSVHHGGGVGMG
                     YSQHAGQVVVCDGSEAAERRIARVLWNDPASGVMRHADAGYAAALRCAKAHGLTLPMA
                     GR"
BASE COUNT          380 a         1011 c         1041 g          412 t
ORIGIN      
        1 gcgcccgtgg caggtcatgc gagcattata tcgagcgcga agggattcag gcccgtggtg
       61 cgcgatgcgg gccctgcggc gcgccgacgg gcctgccagt cggaccccgg aatgcccggg
      121 ctccattccc gcccctcggc cacaggcggc cctgcgtaac cgccgggcac gctcgggcgt
      181 cctagagaca gggcgctgtc ccggcggcgg gcttgccgcc gcctcgtgtg aggggtaatc
      241 tggctgcgcc cccgcgacaa ggcaggaacg gacatgggaa acactctgaa aacggccttg
      301 ctgctcggtt tgatgagcgg cgtgctcctg gtcctgggcg actacttcgg cggccgaggc
      361 gggctggtga tggccttcgg tttcgccgtc ctcatgaact tcgcgtcgta ctggttctcg
      421 gacaagatcg tgctgcggat gtaccgcgcg acgccggtgg ggcccgggca cccgcttttc
      481 cagatcaccg agcggctggc ccagcgggcc ggcctgccga tgccgagggt gttcgtcatc
      541 cccgatccgt cgccgaacgc cttcgccacg gggcgcagtc cgtcgcacgc ggcggtggcg
      601 gccacggagg gcattctgag gctgctcgac gagcgcgaga tcgagggcgt catcgcgcac
      661 gagctggccc acgtgaagca tcgcgacatc ctcgtcgggt cggtggcggc gacgctggcg
      721 gcggccatca tgatgatggc gaggatggcg cagttcgcgg cgttcttcgg cggcggccgc
      781 agcgacgatc gcgagggcgg ctcgaacccc gtcgccctgc tcgcgacggt catcctggcg
      841 ccggtggcgg cgatgctgat ccaggcggcg atctcgcggt cgcgggagtt cggcgcggac
      901 gccggcgggg ccgccatcgc gggcacgccc tacggcctgg tcgacgcgct gaagaagctg
      961 gagtacgcgt cgaagcgcgt tcccctggag gcgaaccccg cgacggcgca catgttcatc
     1021 gtcaagccgt tcagcgtgca cggcctgctg tcgctcttca gcacgcaccc gccgacggag
     1081 gcgcgcatcc gcgcactgct cggccaggcg tagccgccct gagccggggg cgccactcag
     1141 cgtccggcca tcggcagcgt caggccgtgc gccttcgcgc agcggagggc cgcggcgtag
     1201 ccggcgtcgg cgtggcgcat cacgccggac gccgggtcgt tccacaagac ccgcgcgatc
     1261 cggcgctccg ccgcttcgct gccgtcgcac acgacgacct gcccggcatg ctgggagtag
     1321 cccatgccga cgccgccgcc gtggtggacg ctcacccacg tcgcgccgct ggcggtgttc
     1381 agcagcgcgt tcaggattgg ccagtccgac acggcgtccg agccgtcggc catgccctcg
     1441 gtctcgcggt tggggctggc gaccgacccg gcgtcgaggt ggtcgcggcc gatgacgacc
     1501 ggcgccttca gcgcccccga tcgcaccatg tcgttgaagg ccagccccgc caggtggcgc
     1561 tcgccgaggc cgagccagca gatgcgcgac ggcaagccct ggaagctgat gcgccgggcg
     1621 gccatgtcga gccagcgccg cacgccggcg ttgtcgggga agagcgcctt cagcgcctcg
     1681 tcggtgcgat ggatgtcgcc gggatcgccg gagagcgcga cccaccggaa ggggcccttg
     1741 ccctcgcaga agagcgggcg cacgtaggcc ggcacgaagc cggggaacgc gaaggcgtcg
     1801 tccaggccgt ggtcctttgc cgcctgcctg atgttgttgc cgtagtcgac gaccggcacg
     1861 cccatcgcct tgaagtcgag catcgcgcgc acgtgcacgg cgcagctccg gcccgcggcg
     1921 gccctcagcg cctcgtgctc ggcgggcgtc gcgcgcgcgg ccgcccagcg ctcgagcgtc
     1981 cagccggccg gaaggtagcc gttcaccagg tcgtgggcgc ttgtctgatc ggtgacgagg
     2041 cccggcaccg ggccgccggc cctcgcgcgg cggaccagct ccggcaagac ctccgcggcg
     2101 ttgccgagca ggcccaccga cacggcttcc cgccgcgcgc agtgcctccg gatgcgcgcc
     2161 agcgcgtcgt cgagatcggc ggcctggacg tccaggtact tcgtgcgcag gcgcgccgcg
     2221 atccgggccg ggtcgcactc gacaatcagc gacgccgcgc cggcgaaggt ggcggcgagc
     2281 ggctgcgccc cgcccatccc gccgaggcca gccgtcagga tccaccgccc cgcccagtcg
     2341 ccgccgtagt gctgacggcc ggcttcggcg aacgtctcgt acgtgccctg cacgatgccc
     2401 tggctgccga tgtagatcca cgacccggcc gtcatctggc cgtacatgaa gaggcccttc
     2461 cgatccagct cgtggaagtg gtcccaggtc gcccaccggg gcacgaggtt ggagttggcg
     2521 atgagcacgc gaggcgcgtc ggcatgagtg cggaacacgc cgacgggctt gcccgactgc
     2581 acgagcagcg tctcgtcgtc ttccagggcc gtgagcgcgt ccaggatggc atcgaagcag
     2641 gcccagtcgc gggccgcacg gccgatgccc ccgtacacga cgagggcctg gggattctcg
     2701 gcgacctcgg gatcgaggtt gttctggatc aggcggtagg gggcttccgt cagccagctc
     2761 ttgcaggtga gcgccgtgcc gcgcggagcg cggacgacgc ggcttgggtc gtgcctgggg
     2821 tcaatcgcca tggtgctcac ctcc
//
LOCUS       DAOSIF010000415         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_31002_length_2817_cov_3.810644, whole genome shotgun sequence.
ACCESSION   DAOSIF010000415 DAOSIF010000000
VERSION     DAOSIF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31002_length_2817_cov_3.810644"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLT35_11900"
     CDS             <1..395
                     /locus_tag="PLT35_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29973.1"
                     /translation="TIVALLAGLLVGSWWLAGWLGTPWRFTFFAAVLALTVADGTIRV
                     HLLFVERQAPASVRAQLVRTRVWRRALDLAVAFVVALAASLALPDHGLAGILLVGFSA
                     GLAAATWFIEPTTTEAAFPRWREHQLQP"
     gene            complement(532..1563)
                     /locus_tag="PLT35_11905"
     CDS             complement(532..1563)
                     /locus_tag="PLT35_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29974.1"
                     /translation="MHVRRAGPAAAVVLAALASAGGAAPGPPSARDRALAPGDRVWLD
                     AHNCYPDRGAWTNRIERALGAGAPWIAIEQDVAWVRDSSTPARGRSVVSHDDEPRGDE
                     PSLEEHFFARVKPLLERALAEGDRTRWPVLILHLDFKTHEPEHHESVWELLGRYERFL
                     TTAEKPAKDAEIAPLELGPLLVLTESGPGQEDAFSRRVPAGGRFRLFGTVPPPPLSDE
                     QARRMHELGPGALIPSGATSYRRWTNLSWAAVEAGGPDEAGAWTDADLARLRALAGHA
                     HARGLWIRFYTLNGHARTEAQGWSGSYNFGSLDAVRARWRAAIETGVEFAATDQYEAF
                     AEELRLSGR"
     gene            complement(1678..>2817)
                     /locus_tag="PLT35_11910"
     CDS             complement(1678..>2817)
                     /locus_tag="PLT35_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG29975.1"
                     /translation="LRREDAVLSRFPHINVRVFTVLVIVSLPLYALAAIMVLGTGQAQ
                     FREAYGLQLTETAQQVAATIDSYVFRRVIDVSILTKVPDVRAAAAAGNKVPLDIGKAR
                     ALDAAWIARPVATAAASGILGNPASHFLSGVVANDRIYREIIVTDREGRLVAASGVCS
                     DYIQSDEAWWKEAFHDGVQGRVSVSDVTFDESAQSHAIEIAMPVSDPQGEQLAGVLKV
                     VADVRELLAVAAGVRSGRLGEAFIIREDGSVVFSQRGVGGKTRFFAADLLRERMKSFA
                     SGDPQSSVHFSARNQDGRSYLVGVAPSQLGASYPHLDWMVAVTQAEEDLFAPARVQMW
                     RMLALFGLIAGLVLAIAAWFSVRLAASPVGKDTHLSEHPAVPRIE"
BASE COUNT          408 a         1000 c         1003 g          406 t
ORIGIN      
        1 ccaccatcgt cgccctgctc gccggcctgc tcgtgggcag ttggtggctg gccggctggc
       61 tcggcacgcc gtggcgcttc acgttcttcg cggcggtgct ggcgctgacg gtcgccgacg
      121 gcacgatccg cgtgcacctg ctgttcgtcg aacgccaggc accggcatcg gtccgggcgc
      181 aactcgtgcg cacgcgcgtg tggcggcgag ccctcgatct ggcggtggcc ttcgtcgtcg
      241 cgctggcagc cagcctggca ctccccgatc acggactggc cggcatcctg ctcgtcggtt
      301 tcagcgcggg cctggcggct gccacatggt tcatcgagcc gaccacgacc gaggcggcgt
      361 ttccgcgctg gagggagcac cagcttcagc cctgaggccg cgcaatcagg ggctaggcgc
      421 cagacgctcg gcgctaggtg gacagggaga aggggctagg gaccaggcgc tgggcgctag
      481 tcagacagga acaggaggca agggacaagc tcgaggggcc ggggttgccc gtcaccggcc
      541 ggacaggcgc agttcctcgg cgaacgcctc gtactggtcc gtggccgcga actcgacgcc
      601 ggtctcgatc gcggcgcgcc agcgggccct gaccgcgtcg agcgatccga agttgtacga
      661 ccccgaccat ccctgcgcct cggttctggc gtgcccgttg agggtgtaga agcggatcca
      721 gagaccccgg gcgtgcgcgt gcccggccag cgctcgcagg cgcgccagat cggcgtcagt
      781 ccacgccccg gcctcgtccg ggccgccggc ctccaccgcg gcccaggaga gattcgtcca
      841 gcgccggtag ctcgtggcgc cggaggggat cagggcgccg ggccccaact cgtgcatgcg
      901 cctcgcctgc tcgtccgaca gcggcggcgg cggcacggtg ccgaacagcc tgaacctgcc
      961 tccggccggc acgcggcggc tgaatgcgtc ttcctggccc ggcccgctct cggtgagaac
     1021 cagaagcggt cccagctcga gaggcgcgat ttcggcgtcc ttcgccggct tctccgccgt
     1081 cgtcaggaag cgttcgtagc gcccgagcaa ctcccagacg gactcgtgat gttccggttc
     1141 gtgagtcttg aagtccagat gcaggatgag gacgggccaa cgggtccgat cgccttcggc
     1201 cagggcgcgt tcgagcaagg gcttcacgcg ggcgaagaag tgctcctcga gagagggctc
     1261 gtctccgcgc ggctcgtcat catgggacac gacggagcgg ccgcgggccg gcgttgacga
     1321 atcgcgcacc cacgcgacgt cctgctcgat ggcgatccac ggcgccccgg ccccgagagc
     1381 gcgctcgatc cggttcgtcc acgccccgcg atcggggtag cagttgtggg cgtcgagcca
     1441 gaccctgtcc cccggagcca gcgcgcgatc ccgcgcggac ggcggacccg gcgcggcgcc
     1501 gcccgcgctc gcgagggcgg ccagaacgac ggcagcggct gggccggcgc gccggacgtg
     1561 catgcagcct ccggcgcgta ccttagccga ttccctgccg tccgcaaacg ccggcgggag
     1621 cccgggccgg tccgccggtg gcgcgatgcc cccgtgaggc ggaggcggtc acgcgcctta
     1681 ttcgatccgc ggcaccgccg gatgctcgct gaggtgcgtg tccttgccaa ccggcgacgc
     1741 ggccaggcgc accgagaacc acgcggcaat cgccaggacc agcccggcaa tcaggccgaa
     1801 cagggcgagc atgcgccaca tctggacgcg cgcaggggcg aacaggtctt cttcggcctg
     1861 cgtcaccgcc accatccagt ccaggtgcgg gtagctcgcg ccgagctggc tgggcgcgac
     1921 gcctaccagg tacgaccgcc cgtcctggtt cctcgcgctg aagtgcacgc tggactgcgg
     1981 gtctcccgac gcgaagctct tcatgcgctc gcggaggagg tcggccgcga agaacctggt
     2041 cttccccccg acgccccgct ggctgaagac gacggacccg tcctccctga tgatgaacgc
     2101 ctctccgagc cggcccgagc gcacgcccgc cgcgacggcg agcagctcgc ggacgtcggc
     2161 gacgaccttg agcacgcccg caagctgttc cccctgcgga tcgctgaccg gcatggcaat
     2221 ctcgatcgcg tgggactgcg cgctctcgtc gaaggtgacg tcgctcacgg acacgcgccc
     2281 ctgcacgccg tcgtggaagg cctccttcca ccaggcctcg tcgctctgga tgtagtcgct
     2341 gcacaccccg gacgcggcca cgagccggcc ttcgcggtcg gtgacgatga tctcgcggta
     2401 gatccggtcg ttggcgacaa cgccgctcag gaagtgcgag gccgggttgc cgaggatgcc
     2461 ggacgcggcg gccgtcgcca ccggccgcgc gatccatgcg gcatcgagcg cgcgggcctt
     2521 gccgatgtcg agcgggacct tgttgcccgc ggctgcagcg gcgcggacat cgggcacctt
     2581 ggtcaggatg ctgacgtcga tcacacgccg gaagacgtag gagtcgatgg tggccgcgac
     2641 ctgctgggcg gtttcggtca gctgcagccc gtacgcctcg cgaaactggg cctggccggt
     2701 gcccaggacc atgatcgcgg ccagcgcgta cagaggcagg ctgacgatga cgaggaccgt
     2761 gaagacccgg acgttgatgt gaggaaagcg agataacacg gcgtcctccc tccgtag
//
LOCUS       DAOSIF010000416         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_31142_length_2808_cov_4.152924, whole genome shotgun sequence.
ACCESSION   DAOSIF010000416 DAOSIF010000000
VERSION     DAOSIF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31142_length_2808_cov_4.152924"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /gene="lexA"
                     /locus_tag="PLT35_11915"
     CDS             <1..502
                     /gene="lexA"
                     /locus_tag="PLT35_11915"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOG29976.1"
                     /translation="GLSSLATVHKHLTNLQEKGFIRRAWNRSRSVEMVPTRGGGRVLE
                     LPLLGYVAAGVPIEAVATPETIAVPDDLVGKRDTYVLKVRGDSMIDEHIRDGDFVIVE
                     DRKTADNGEMVIALLGGSDVTLKKFYRENGTIRLQPANPAMQPIVVAAGDVQIQGVVV
                     GVMRRY"
     gene            546..863
                     /locus_tag="PLT35_11920"
     CDS             546..863
                     /locus_tag="PLT35_11920"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOG29977.1"
                     /translation="MAEQPRQVNFTIVPADAPDTPRTYANFCGVSHTPFDFTLTFCEV
                     QPLSERDVQAAQDEHVIRAPVRARVVVPVQIVASLAAALQEQLRAYNDAQAAGGHLKG
                     PVH"
     gene            865..1581
                     /gene="nth"
                     /locus_tag="PLT35_11925"
     CDS             865..1581
                     /gene="nth"
                     /locus_tag="PLT35_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG29978.1"
                     /translation="MKTAEDVRAIIDVLARQHPGADTELRYATPFELLVATILSAQST
                     DAGVNRVTPALFAAYPDAGALATAPLEEVERLVHATGFYRQKARALLGMSAALVERHG
                     GEVPRTMEALVRLPGVGRKTANVVLGHALGVPGFPVDRHVLRVSARLGIATSDDPERV
                     EQQLTGALPPALWTLASDTLILHGRRICKSEPLCRQCAAAAMCDYRRRPARPAPARAA
                     RKPARTKASRAKAPATRRRS"
     gene            1578..1985
                     /locus_tag="PLT35_11930"
     CDS             1578..1985
                     /locus_tag="PLT35_11930"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOG29979.1"
                     /translation="MTRARFERLVAEAIRTIPERFRDELRNIAVIVEDEPSDDLLDEL
                     GIAPPDTLFGLYHGTPLTERSWDYGNALPDRITLFQGPIEDSSESEDDVVVAIGETLI
                     HEVGHYFGLSEEELEAIEERYWRSFDPPEGERA"
     gene            1982..2779
                     /gene="rsmA"
                     /locus_tag="PLT35_11935"
     CDS             1982..2779
                     /gene="rsmA"
                     /locus_tag="PLT35_11935"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG29980.1"
                     /translation="MKPRRRFGQHFLQPPWIARVLAVAAFGPGDRVLEIGPGRGALTG
                     PLSERVSELAAVEIDRDLAAFLRGRLPARVRIIEGDVLEADPAALFGPPGGPPWRVAG
                     NLPYCASSPIMFRLLELARTTARVRDATVMLQREVADRMASPPGSRHYGALSILVQLD
                     ADVERLLTLPPQAFKPPPDVWSALVRLHFRPPAVAVRDRRQFEAMVRSLFSGRRKMLS
                     NAVKPFASARGLDGAAVARDAGLDPTRRPETLDLRDLSRLADLLCRT"
BASE COUNT          382 a         1019 c         1011 g          396 t
ORIGIN      
        1 cggcctgtcg tcgctcgcga cggtgcacaa gcacctgacg aacctgcagg agaaagggtt
       61 catccggcgg gcgtggaacc ggagccgctc ggtcgagatg gtgccgacgc ggggcggagg
      121 ccgcgtcctc gagctgcccc tgctggggta cgttgcggcc ggcgtgccca tcgaggcggt
      181 cgcgacgccc gagacgatcg cggtgcccga cgatctggtc ggcaagcgcg acacctacgt
      241 gctcaaggtg cggggcgact cgatgatcga cgagcacatc cgggacgggg atttcgtcat
      301 cgtcgaggac cgcaagaccg ccgacaacgg cgagatggtc atcgcgctcc tcggcggatc
      361 ggacgtcacg ctgaagaagt tctaccggga gaatggcacc atccggcttc agcccgccaa
      421 cccggcgatg cagccgattg tcgtggcggc cggggacgtg cagatccagg gcgtcgtggt
      481 cggcgtgatg cggaggtact gacgccggac ctggcgcgcg ccggtcccgc agcggagaat
      541 gactcatggc tgaacagccg cgccaggtga acttcaccat cgtgcccgcc gacgcgcccg
      601 acacgccgcg gacgtacgcg aacttctgcg gcgtctcgca cacgccgttc gacttcacgc
      661 tgacgttctg cgaggtgcag ccgctgtcgg agcgcgacgt gcaggcggcc caggacgagc
      721 acgtcatccg ggccccggtg cgcgcgcgcg tcgtcgtccc cgtgcagatc gtggcgagcc
      781 tcgcggcggc cctgcaggag cagctgcgcg cctacaacga cgcccaggcc gccggcgggc
      841 acctcaaagg accggtgcac tagcgtgaag acggcggagg atgtccgggc gatcatcgac
      901 gtgctcgcgc gccagcaccc cggcgccgac accgagctcc gctacgcgac ccccttcgag
      961 ctgctcgtcg ccacgatcct ctcggcgcag tcgaccgacg cgggcgtgaa ccgcgtcacg
     1021 cccgcgctgt ttgccgcgta ccccgatgcc ggcgccctcg ccaccgcgcc gctcgaggag
     1081 gtcgagcgcc tcgttcacgc gacgggcttc taccggcaga aggcccgcgc gctgctcggc
     1141 atgtcggccg cgctcgtcga acgccacggc ggcgaggtgc cgcggaccat ggaagccctg
     1201 gtcaggctgc cgggcgtggg ccgcaagacg gcgaacgtcg tgctcggcca cgccctcggc
     1261 gtgcccggct tcccggtgga tcggcacgtg ctgcgcgtca gcgcgcgcct cggcattgcc
     1321 acgtccgacg atcccgagcg ggtggagcag cagctgacgg gcgccctgcc gccggccttg
     1381 tggacgctgg cctcggacac gctgatcctt cacggccggc ggatttgcaa gagcgaaccg
     1441 ctctgccggc agtgcgcggc ggcggcgatg tgcgactacc ggcggcggcc cgcccgtccg
     1501 gccccggccc gcgccgcgcg caagccggcc cgcacgaagg cctcgcgggc gaaggcgccc
     1561 gccacgcggc ggcgctcgtg acccgcgccc gcttcgagcg cctcgtcgcc gaggcgatcc
     1621 gcaccatccc ggagcgcttc cgggacgagt tgcgcaacat cgccgtcatc gtggaagacg
     1681 agccctccga cgatctgctc gacgagctgg gcatcgcccc cccggacacg ctgttcgggc
     1741 tctaccacgg caccccgctc accgagcgct cgtgggacta cggcaatgcg ctgcccgatc
     1801 gcatcacgct gttccagggg ccgatcgagg actcgagcga gagcgaggac gatgtcgtcg
     1861 tggcgattgg cgagacgctg attcacgagg tgggccacta cttcgggctg tccgaggagg
     1921 aactcgaggc gatcgaggag cgctactggc gctcgttcga tccgccggaa ggcgaacggg
     1981 cgtgaagccg cgccgccggt tcggccagca cttcctccag ccgccctgga tcgcgcgggt
     2041 gctcgccgtc gcggcgttcg ggccaggcga tcgcgtcctc gagatcggcc cgggccgcgg
     2101 cgcgctgacg ggcccgctga gcgagcgcgt ctcggagctg gcggccgtgg agatcgaccg
     2161 cgacctcgcg gccttcctgc gcggccggct cccggcgcgc gtgcggatca tcgaaggcga
     2221 tgtgctcgag gccgatccgg ccgcgctgtt cggcccgccg ggcgggccgc cgtggcgcgt
     2281 ggcggggaat cttccctact gcgcctcctc tccgatcatg ttcaggctcc tcgagctggc
     2341 ccgcaccacc gcgcgcgtgc gagacgcgac cgtgatgctc cagcgcgaag tggccgaccg
     2401 catggcgtcg ccgccggggt cgcgccacta cggcgcgctt tcgatcctgg tgcagctcga
     2461 cgccgacgtc gagcggctcc tcacgctgcc gccgcaggcg ttcaagcctc cgccggacgt
     2521 ctggtccgcg cttgtccggc tgcacttccg gccgccagcc gtcgcggtgc gcgaccggcg
     2581 gcagttcgag gcgatggttc gatcgctttt ctcggggcgg cgcaagatgc tgtcgaacgc
     2641 ggtgaagccc ttcgcgtcgg cccggggcct cgacggggcc gccgtcgcgc gcgacgcggg
     2701 cctggatccg acgcgccggc ccgagacgct ggatctccgg gacctgtcga ggcttgccga
     2761 cctcctctgt cgcacctgag cggtgccgct caggtgcgac agaggagg
//
LOCUS       DAOSIF010000417         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_31333_length_2795_cov_3.401825, whole genome shotgun sequence.
ACCESSION   DAOSIF010000417 DAOSIF010000000
VERSION     DAOSIF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31333_length_2795_cov_3.401825"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLT35_11940"
     CDS             complement(<1..802)
                     /locus_tag="PLT35_11940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG29981.1"
                     /translation="MIRKLALLLLVAVVLAPACRKVAPSAVRRSDGAAAPAQAAAPGE
                     PAAQPVPAELPQVVARVNGQDVQRSELDMAVKAVEERAGSAVSPEQRDALYRQVLDRL
                     VGYHLLVQEARRRKIAAPAADVDDQIADMRRQFPNEAAFSDLLQSRGVTLEQLRADTA
                     DTLAVNRMLQSEIEPGLAVTEAEARAFFEQNQARFRQGDAVRASHILVRVEAGAGAAA
                     KAQARARAERLLAELKQGAAFSDLAKRESQDPGSAANGGDLGFLTRGDM"
     gene            1101..1661
                     /locus_tag="PLT35_11945"
     CDS             1101..1661
                     /locus_tag="PLT35_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29982.1"
                     /translation="MPVRLFLGNLPYSATEADLRAHVSAVAPPLSIVLPMDRETGRPR
                     GFAFVDYPDLAAATEVIRRLNGQPFLGRTLSVSEARPREERGPRPPSTAGAPFAGGYR
                     APAPPADSAARRERNFGPPAPPKGRRAGPAKGRGEGGPKGPIKIRGGGRVSDLDEEKA
                     DFDLGDDFDDFATSLPSGHDDDGGDA"
     gene            1767..2423
                     /locus_tag="PLT35_11950"
     CDS             1767..2423
                     /locus_tag="PLT35_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG29983.1"
                     /translation="MTSAAFGLLTLGLLVPSIVLHEVAHGWVSLRLGDPTARNAGRLS
                     LNPLRHIDLFGTVLLPLMLWSSGAPVFGYAKPVPINPAYYRDVRRGQLRASLAGPAAN
                     LLLAGAGSAAAWLAWTLGARDCRPLAWAWLFAGAFVEVNLVLMFFNLIPIPPLDGSSI
                     VPLFLSDSALPTYYRLQRYAFPLLLVVCWGVPALSGVDPIGAYLRLSVPLLFNVLVPQ
                     "
     gene            2515..>2795
                     /locus_tag="PLT35_11955"
     CDS             2515..>2795
                     /locus_tag="PLT35_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29984.1"
                     /translation="MPAGWSALLAAPALAQAVRRERAAALGFSANRLARIAPVMEAFV
                     RDGSAGDYRWDGAASTFLQNDLRQAPALLAVGRRTPCDVKISGKARRWFP"
BASE COUNT          355 a         1044 c          967 g          429 t
ORIGIN      
        1 ccatgtcacc gcgggtgaga aacccgaggt cgccgccgtt tgcggcgctg ccgggatcct
       61 gcgactcgcg tttcgccaga tccgagaacg cggcgccttg cttcagttcc gccagcaggc
      121 gctccgccct ggccctggcc tgcgccttgg ccgccgcacc cgcgccggcc tcgacgcgga
      181 cgaggatgtg gctcgcccgc acggcgtctc cctgccgaaa ccgggcctgg ttctgttcga
      241 agaacgccct ggcctccgcc tccgtcaccg cgagcccagg ctcgatctcc gactgcagca
      301 tccggttgac ggcgagcgtg tcggccgtgt ccgcgcgcag ctgctcgagc gtgacgccgc
      361 gcgactgcag caggtccgag aacgccgcct cgttggggaa ctgccgcctc atgtcggcga
      421 tctgatcgtc cacgtccgcg gccggcgccg cgatcttccg cctgcgggct tcttgaacaa
      481 gaaggtggta gccgacgagc cggtcgagga cctgacggta tagcgcgtcg cgctgttcgg
      541 gcgaaacggc gctgccggcg cgctcctcga cggccttgac ggccatgtcg agctcggacc
      601 gctgcacatc ctggccgttg acgcgggcga caacctgcgg caactccgcg gggaccggtt
      661 gtgcggccgg ctcgcccggg gccgccgcct gcgccggcgc ggcagcgccg tcggagcggc
      721 gcaccgccga gggcgcgacc ttcctgcagg ccggcgccag gacgacggcc acgagcagca
      781 gcagcgcgag cttccggatc acgggatctc ctgctcgagg aacgcctcac gctaccaaag
      841 gaccccggga tgtgcaaggc gaggtcaggc gccgatacca ccggggacag gcccggcagc
      901 ggccgccatc ccgctctggc gtgcagacgg gcggcggccg ggaagcgcgg cgcgaggttg
      961 cgcccgccat ccagctttgg tagagtctag cgacggcatc agccctgagc cttgaccgcc
     1021 cattcctccc gagcgtctgc gcgcatccct ccagccacaa cacggccgag cgccgtataa
     1081 ccacgaggtg agataccagc gtgccagttc ggttgtttct cggcaatctc ccctacagcg
     1141 ccacggaagc ggacctccgg gcgcacgtgt ccgccgtggc cccgccgctc tccatcgtct
     1201 tgccgatgga ccgcgagaca gggaggccgc gagggtttgc cttcgtggac tacccggatc
     1261 tcgccgcggc gacggaggtc attcgcaggc tgaatggcca gccattcctc ggccggaccc
     1321 tgtccgtgag cgaggctcgg ccgagggagg agcggggacc gcgcccgccg tcgaccgccg
     1381 gcgcgccctt cgcgggcggc taccgcgcgc ccgctccgcc cgctgacagc gccgcccgcc
     1441 gcgagcggaa tttcgggcct ccggccccgc cgaaaggtcg gcgcgccggc ccggcgaaag
     1501 ggcggggaga gggcggtccg aagggcccga tcaagatcag gggcggcgga cgggtgtcgg
     1561 acctcgatga agagaaggcc gatttcgatc tgggagacga cttcgacgac ttcgccacca
     1621 gcctgccgtc cgggcacgac gacgacggcg gcgacgcctg aagcgcgcag agtcctgcgc
     1681 tgccgcaccg agcagccggc cggttctcgt ccgcgcggcg gggccgtccg gcgctccggc
     1741 tgcggctctc ggctggacgc ccggacgtga cgtccgccgc cttcggcctc ctcacgcttg
     1801 gcctgctcgt cccgtccatc gtgttgcacg aggtcgcgca cggctgggtg tcgctccggc
     1861 tcggcgatcc cacggcgcgc aacgccggcc ggctttcgct caacccgctg cgccacatcg
     1921 atctcttcgg cacggtgctg ctgccgctca tgctctggag ctccggcgcg cccgtattcg
     1981 gctacgccaa gccggtgccc atcaatccgg cctattacag ggacgtgcgc cggggccagt
     2041 tgcgcgccag cctcgcgggt ccggcggcga acctgctgct tgcgggcgca ggaagcgcgg
     2101 ccgcgtggct cgcgtggacg ctcggcgccc gtgactgccg cccgctggcc tgggcgtggc
     2161 tgttcgcggg cgcgtttgtc gaagtgaacc tggtcctgat gttcttcaac ctgatcccga
     2221 ttccgcccct cgacggatcg agcattgtcc cgttgttcct ctcggactcg gccctcccta
     2281 cgtactaccg gctccagcgg tatgcctttc ctctcctgct cgtggtgtgc tggggcgtcc
     2341 cggccctgtc gggagtggac ccgatcggcg cctacctccg cctcagcgtg cccctgctct
     2401 tcaacgtcct agtgccgcag taaggccttc cggcggcctc gccgacgcct gccgcgcttg
     2461 cgccggcacc ggccggccgc cgagagagga gcccctcgcg gcacacgagc cgcgttgccg
     2521 gccgggtggt cggccctgct ggccgctccc gcgctcgcgc aagccgtgag gcgggagcgg
     2581 gccgctgcct tgggcttctc cgccaaccgc ctcgcccgca tcgcgcctgt gatggaggcg
     2641 ttcgtccgcg acggatcggc gggcgactac cgctgggacg gcgcggcctc cacgttcctc
     2701 cagaacgatc tcagacaggc cccggccctg ctcgctgtgg ggcgccggac gccgtgcgac
     2761 gtgaagatct cgggcaaggc aaggcgctgg tttcc
//
LOCUS       DAOSIF010000418         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_31715_length_2768_cov_2.932547, whole genome shotgun sequence.
ACCESSION   DAOSIF010000418 DAOSIF010000000
VERSION     DAOSIF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31715_length_2768_cov_2.932547"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..286
                     /gene="rpsO"
                     /locus_tag="PLT35_11960"
     CDS             17..286
                     /gene="rpsO"
                     /locus_tag="PLT35_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG29985.1"
                     /translation="MALTKDRKSELIKSFERHDGDTGSPEVQIAILSERINYLTEHFK
                     VHAKDHHSRRGLLMLVGQRRRLLDYVKSKDPKRYAAIIERLGIRK"
     gene            343..2580
                     /gene="pnp"
                     /locus_tag="PLT35_11965"
     CDS             343..2580
                     /gene="pnp"
                     /locus_tag="PLT35_11965"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG29986.1"
                     /translation="MHKRELTLGAGTLSIETGRLARQANGSAVVTFGDTVVLVTACYA
                     PSERVGIDFLPLTVDYREYTYASGRIPGGFFKREGKATEKEVLTSRCIDRPIRPLFPD
                     TWHHETQVIALVLSADNEYDPDVVAITGASAALAFSEIPFRKTLAAVRVGYVDDAFVI
                     NPTYAQRKQSPLDIIVAGSKDSLVMVEAGAREVSEEVIVRALEAAHEAIRGVVAMIDD
                     MAREVGKAKRQAAPKAIDPALAREIEAAVYGPLAEAMRIRGKLENYRKVDEIIGGCLA
                     SIPDEDAERKAAAKAVLSGLKERVLRDEILERGVRLDGRAFDEIRPIWCEVGVVPRTH
                     GSAVFTRGETQALVTTTLGTSDDQQKIETVGGEFYKRFMLHYNFPPFSVGEVAFMRGP
                     GRREVGHGALAERALAPMLPSEEQFPYTVRVVSDILESNGSSSMASVCGGSLSLMDAG
                     VPIKAPVAGIAMGLVMNEETGKYAVLTDIAGAEDHYGDMDFKVAGTREGVTALQMDIK
                     VAGVTAEVLQQALAQARKGRLEILETMAGALAAPRDDVSSHAPRIVTIKIPVDKIRDV
                     IGPGGKMIRSIIERTGVKIDVEDDGRVNVASSDESSSKKAVAIIMELTATAELNKTYL
                     GKVQRITDFGAFVEIIPGTDGLLHVSEIARHRVQDVRDELNEGDQVLVKVISIDPSGK
                     IRLSRKALLAAEDGEAAPAGDAEGEGAKGGAEEPRAHRGAPRPHEHRDRGARGGRPGG
                     RDRRR"
BASE COUNT          477 a          937 c          997 g          357 t
ORIGIN      
        1 gtggaggcga cgacacgtgg cactgaccaa agaccggaag tccgagctca tcaagtcctt
       61 cgaacgtcac gacggcgaca ccggctcgcc tgaggtccag atcgcgatcc tgagcgagcg
      121 catcaactat ctgaccgagc acttcaaggt tcacgccaag gaccaccact cgcggcgcgg
      181 cctgctgatg ctggtcggcc agcgccggcg cctgctcgac tacgtcaaga gcaaggaccc
      241 gaagcgctac gcggcgatca tcgagcgcct gggcatccgc aagtaaccgc cgggacgtcg
      301 gtgcccggct gagccatgcc cgggccggag agaagcacga ccatgcacaa gcgagaactc
      361 actctgggcg ccggcacgct gtcgatcgaa accgggcgcc tggccaggca ggccaacggc
      421 tcggctgtcg tcaccttcgg cgacaccgtg gtgctcgtta cggcgtgcta cgccccgtcc
      481 gagcgggtgg gcatcgattt cctcccgctc acggtcgact accgggagta cacatacgca
      541 tcggggcgca tccccggggg cttcttcaag cgggagggca aggcgaccga gaaggaagtc
      601 ctgacgagcc ggtgcatcga ccggcccatc cggccgctct tccccgacac ctggcaccac
      661 gagacgcagg tcatcgcgct cgtgctctcg gccgacaacg agtacgaccc ggatgtcgtc
      721 gccatcacgg gcgcgtcggc ggcgctggcc ttctcggaga tcccgttccg gaagacgctg
      781 gctgcggtgc gcgtcgggta cgtggacgat gcgttcgtca tcaacccgac ctacgcccag
      841 cgcaagcagt cgcccctcga catcatcgtc gccggcagca aggacagcct ggtgatggtc
      901 gaggccggcg ccagggaggt cagcgaggag gtcatcgtgc gggccctcga ggccgcgcac
      961 gaggccatcc gcggcgtcgt ggcgatgatc gacgacatgg cgcgcgaggt gggcaaggcc
     1021 aagcgccagg ccgcgcccaa ggccatcgat cccgcgctgg cgcgcgaaat cgaggccgcg
     1081 gtctacgggc cgctggccga ggcgatgcgc atccgcggca agctcgagaa ctaccggaag
     1141 gtggacgaga tcatcggcgg ctgcctggcg tccatcccgg acgaggacgc cgagcggaaa
     1201 gccgcggcga aggccgtgct ctcgggcctc aaggaacggg tgcttcgcga cgagatcctc
     1261 gagcgcggcg tgcgcctgga cgggcgcgcg ttcgacgaga tccgcccgat ctggtgcgag
     1321 gtcggggtgg tgccgcgaac gcacggctcg gcggtcttca cgaggggcga gacccaggcg
     1381 ctcgtgacga ccacgctggg cacgtccgac gatcagcaga agatcgagac ggtgggcggc
     1441 gagttctaca agcggttcat gctccactac aacttcccgc cgttctccgt cggcgaggtg
     1501 gccttcatga ggggcccggg ccgccgcgag gtcgggcacg gcgccctggc cgagcgcgcg
     1561 ctcgccccga tgctgccgtc cgaggagcag ttcccctaca cggtgcgcgt cgtgtcggac
     1621 atcctcgagt cgaacggctc ctcgtcgatg gccagcgtgt gcggaggcag cctctcgctg
     1681 atggacgcgg gcgtgccgat caaggccccg gtggccggca tcgcgatggg cctcgtgatg
     1741 aacgaggaga ccggcaagta cgccgtgctc acggacatcg ccggcgccga ggaccactac
     1801 ggcgacatgg acttcaaggt ggccggcacc cgcgagggcg tgacggcgct ccagatggac
     1861 atcaaggtgg ccggcgtcac cgccgaggtg ctgcagcagg ccctcgcgca ggcgcgcaag
     1921 ggccgcctcg agatcctcga gacgatggcc ggcgcgctgg cggccccgcg cgacgacgtc
     1981 tcgtcgcacg cgccccgcat cgtgacgatc aagattcccg tcgacaagat ccgcgacgtc
     2041 atcgggcctg gcggcaagat gatccgcagc atcatcgagc ggacgggcgt caagatcgac
     2101 gtcgaagacg acgggcgcgt caacgtggcg tcctcggacg agagctcgtc gaagaaggcc
     2161 gtcgccatca tcatggagct caccgccacg gcggagctca acaagaccta tctcggcaag
     2221 gtgcagcgga tcaccgactt cggcgccttc gtcgagatca tccccgggac cgacggcctc
     2281 ctgcacgtgt cggagatcgc caggcaccgc gtgcaggacg tgcgcgacga actgaacgag
     2341 ggcgaccagg tcctcgtgaa ggtcatcagc atcgacccgt cgggcaagat ccggctgagc
     2401 cggaaggcgc tcctggcggc ggaagacggc gaggcggcgc ccgcgggcga cgccgagggc
     2461 gaaggcgcca agggcggcgc cgaagagccc cgggcgcaca ggggggcgcc caggccgcac
     2521 gagcaccggg accgaggcgc gcgcggcggg cggccgggcg ggcgcgaccg gaggcggtag
     2581 cctcgcgccc tgcgcgaggg ccggatcgaa cttcccggcc ctctcgcgcg tcagaaggat
     2641 cgggagggcc tctcacatgg caatcggagt gctggctcgc cgcgccgctg ccgccgggct
     2701 gctgtgcgcg gccgcgctgg gtgccgggag catcgggtcc ggacaggagc agggacgccg
     2761 cgagatca
//
LOCUS       DAOSIF010000419         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_31749_length_2766_cov_3.368868, whole genome shotgun sequence.
ACCESSION   DAOSIF010000419 DAOSIF010000000
VERSION     DAOSIF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31749_length_2766_cov_3.368868"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1622)
                     /locus_tag="PLT35_11970"
     CDS             complement(108..1622)
                     /locus_tag="PLT35_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG29987.1"
                     /translation="MAHEASAPAAHPASGLDTEFQRGLGLYDSTMLVVGAMIGSGIFI
                     VSANMARGIGSAGWLLAAWVITGVLTVAGALSYGELAAMMPRAGGQYVYLREAYSPLW
                     GFLYGWTLFTVIQTGTQAAVAIAFARYFGVLVPWISEQRYLVPPVHLSRGYAVSLSTT
                     QFVGILSIALLTWTNSRGLRYGKIIQNLFTTAKTAGVVALIAIGLFLGWNADAVAANL
                     GHFWTARGVEPVAPGVVATSAFGLFIALAVAQTGSLFSSDAWNNVTFTAGEVKDPRRN
                     IPLSLALGTSAVIALYLLVNVAYLVTLPLDAIQRAPADRVATAMLERVLPGLSVVAMA
                     VTIMISTFGCNNGLILAGARAYYAMAKDGLFFGAAGRLNDARVPAVGLALQGVWATLL
                     VLPRTYDPATGTYGNVYSNLLDYVISANLVFYVLTILAVFRLRATRPDAERPYRAFGY
                     PLVPALYVIGAATILVVLVVYRPATTWPGFVIMFLGLPVYFFWKGRRTEGPGRG"
     gene            1705..>2766
                     /locus_tag="PLT35_11975"
     CDS             1705..>2766
                     /locus_tag="PLT35_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOG29988.1"
                     /translation="MPPSLVVKLDLVQTLALASVILFVGYGVRRRVGVLDRYNIPAPV
                     VGGFLFAALATALRLQGVAAFDFDITLQAPLMIAFFTTIGLGASLALLKVGGPQVLLF
                     WALASALAALQNAVGVGLAKALGVHPFLGLIAGSITMTGGHGTGAAFGKLMEDQYAFA
                     GGVTLAMAAATFGLVSGGLIGGPVATRLITRRRIAVPGKGAAAGSASPPAPPAADAAS
                     VKAMGLDSEIDTEAAGEAPTAYTLLKTMTVILVAMWAGSLLSGWLGRHVTLPAYIWAM
                     IIAAVIRNGDDVLRVVRLNQRVVDDLGAKAIERARGHAAGQRVDQDRLDGCARQHRRQ
                     GGQGRVVGEGGDRHVLVPCT"
BASE COUNT          422 a          941 c         1015 g          388 t
ORIGIN      
        1 gagatcgcgc tggccgaccc gggcgacctg gatcgcttcg atctggcgcc cgagaccctc
       61 cgggtgattc gggcgggcct cgccctcgca ggcgagtcgg cggtcggcta gcctcgcccc
      121 gggccctccg ttcgccggcc cttccagaag aagtagacgg gcaatcccag gaacatgatg
      181 acgaagcccg gccaggtcgt ggccgggcgg tacacgacca gcaccacgag gatcgtcgcg
      241 gcgccgatca cgtagagagc cgggacgagg ggatagccga acgcccggta gggccgctcg
      301 gcgtccggtc tggtcgcccg cagcctgaac accgcgagga tcgtgaggac gtagaagacc
      361 aggttggccg agatcacgta gtccaggagg ttgctgtaga cgttgccgta cgttcccgtc
      421 gcggggtcgt acgtgcgcgg caggacgagg agcgtcgccc acacgccctg cagggcgagc
      481 ccgacagcgg ggaccctggc gtcgttgagg cggcccgccg ccccgaagaa caggccgtcc
      541 ttcgccatgg cgtagtacgc gcgggcgccg gccagaatca ggccgttgtt gcagccgaag
      601 gtcgagatca tgatcgtgac cgccatcgcg accacgctga ggcccgggag gacgcgctcg
      661 agcatcgcgg tcgcgacgcg gtcggcgggc gcccgctgga tcgcgtcgag cggcagcgtc
      721 accaggtacg cgacgttcac cagcaggtag agggcgatga cggcgctcgt gccgagcgcg
      781 agcgagagcg ggatgttgcg ccgcgggtct ttgacctcgc cggcggtgaa ggtgacgttg
      841 ttccacgcgt cggatgagaa gagcgacccg gtctgcgcca cggccagcgc gatgaacagc
      901 ccgaacgcgc tcgtggcgac gacgcccggc gccaccggct cgacgccgcg cgccgtccag
      961 aaatgcccga ggttcgccgc caccgcgtcg gcgttccagc cgaggaacag gccgatggcg
     1021 atgagcgcca cgacgcccgc ggtcttcgcc gtcgtgaaca ggttctggat gatcttgccg
     1081 tagcggaggc cgcgcgagtt cgtccaggtg agcagggcaa tcgagagaat gccgacaaac
     1141 tgcgtggtcg acagcgacac cgcgtagccc cgggagaggt ggacgggcgg gacgagatag
     1201 cgctgctccg agatccaggg cacgagcacc ccgaagtacc gggcaaacgc gattgcgacg
     1261 gcggcctgcg tgccggtctg gatcacggtg aagagggtcc agccgtacag gaacccccag
     1321 agcggcgagt aggcctcgcg caagtagacg tactgtccgc cggcgcgggg catcatggcc
     1381 gccagctcgc cgtacgagag cgcgcccgcc accgtcagga cgcccgtgat cacccaggcg
     1441 gccagcagcc agcccgcgct cccgatgccg cgggccatgt tcgccgacac gatgaagatc
     1501 cctgacccga tcatcgcccc gacgaccagc atcgtcgagt cgtagagacc gagcccgcgc
     1561 tggaactccg tgtcgaggcc gctggcggga tgcgctgcgg gcgcggacgc ctcgtgggcc
     1621 atgtgcgctc ctccgaccgt ctcgcggttg gcgtttgccg gcggacgaat atatcatgcg
     1681 cggagcccgg ctccccggcc cgacatgccg ccatccctcg tcgtgaagct cgatctcgtg
     1741 cagacgctgg cgctcgcgtc ggtgatcctg tttgtcggct acggcgtgcg ccggcgagtc
     1801 ggggtccttg accgctacaa catcccggcg ccggtggtcg gaggcttcct gttcgccgcg
     1861 ctggccacgg cgctgcgcct gcagggcgtc gcggcgttcg acttcgacat cacgctgcag
     1921 gcaccgctga tgatcgcctt cttcacgacg atcggcctgg gcgcgagcct cgcgctgctg
     1981 aaggtgggcg ggccgcaggt cctgctcttc tgggcgctgg cctccgcgct ggcggcgctc
     2041 cagaacgccg tcggcgtcgg gctggcgaag gccctcggcg tgcatccgtt cctggggctg
     2101 atcgcgggct ccatcacgat gacgggcggc cacggcacgg gggctgcgtt cggcaagctg
     2161 atggaggacc agtacgcctt cgccggcggc gtgactctcg cgatggcggc ggccacgttc
     2221 ggcctcgtga gcggcggcct catcggcggc cccgtcgcca ccaggctcat cacgcgccgg
     2281 cggatcgccg ttccggggaa gggcgccgcg gccggatcgg cctccccgcc cgcaccgccg
     2341 gcggcggacg cggcgtcggt gaaggccatg ggcctcgatt ccgagatcga caccgaggcg
     2401 gcgggcgagg ccccaacggc ctacacgctg ctcaagacga tgacggtgat cctggtcgcg
     2461 atgtgggccg ggtcgctgct gagcggatgg ctcggccggc atgtcaccct gccggcgtac
     2521 atctgggcga tgatcatcgc cgctgtcatc cgcaacggcg acgacgtcct gcgggtcgtc
     2581 cggctgaacc agcgggtggt ggacgacctc ggcgcgaaag ccatcgagag ggcccgtggt
     2641 cacgcggccg ggcagagggt cgaccaggac cggctcgacg gctgcgcgcg acaacaccgc
     2701 cgacagggtg ggcagggccg ggtcgtcggg gaaggcggcg atcgacacgt cctcgtgccg
     2761 tgcact
//
LOCUS       DAOSIF010000420         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_31871_length_2757_cov_3.007772, whole genome shotgun sequence.
ACCESSION   DAOSIF010000420 DAOSIF010000000
VERSION     DAOSIF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31871_length_2757_cov_3.007772"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..921)
                     /locus_tag="PLT35_11980"
     CDS             complement(46..921)
                     /locus_tag="PLT35_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG29989.1"
                     /translation="MPSVPARAAILLTALLFSTGGAAIKAVSLTSWQIASLRSGVAAA
                     VLLLAVRKLPRLTAPTALVGAAYAATLVTFVTANTLTTAANAVFLQAAAPLYIALLAP
                     WLLGEHLRRRDLPVLAAIAAGMALLVAGSGAPTVTAPNRALGNAVGLVSGVCWSLTVM
                     GLRWIAKRDPHAGGIAAGSAALTGNIIACLATLPLALPLHAVAPRDAAGIAYLGLFQV
                     GLAYVLLTRAIRHVPAVDASLLLLLEPALSPLWAWLLHREAPGFWPLLGGACILGATV
                     RKTWREAKASLDGGT"
     gene            complement(1026..1274)
                     /locus_tag="PLT35_11985"
     CDS             complement(1026..1274)
                     /locus_tag="PLT35_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017034990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HOG29990.1"
                     /translation="MALWINQDCISCGACEPECPNEAITQGDSIYVIDPAKCTECVGA
                     YDTSKCVDVCPVDGCITQDPNYVETREQLEAKYHALHG"
     gene            complement(1433..>2757)
                     /locus_tag="PLT35_11990"
     CDS             complement(1433..>2757)
                     /locus_tag="PLT35_11990"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HOG29991.1"
                     /translation="GAARRDDEGCAMFHPPRKYRHRDVQGHPLRPETLMMGYGYDPQF
                     SEGAVKCPIFQTSTFTFKTAEDGKSFFQLAYGLREQGPAEEPGLIYSRVNNPDLEILE
                     ERLGLWDEAESGLAFSSGMAAISTTMFSVLRPGDVLLASAPVYGGTEFLIENILPQFG
                     IKRVWFGAGSLEPGLADAVETARGLGRVGLIYVETPANPTNGLVDIAACAAAARSLRN
                     ADGRRPLVAVDNTFLGPLWQRPLRHGADFVIYSLTKYVGGHSDLIAGACLGTESAIAP
                     VRGLRTILGTIADPHTCWLLLRSLETLKLRMTGSMENARRVAEFLAGHPKVKAVHYLG
                     FLGPADPQLGLYQRQCEAPGSTFSFEVDGGEAEAFRLLNRLEVIKLAVSLGGTESLIE
                     HPATMTHSDIPPGRQLEMGITPSMVRLSVGLEHPEDLIADLRQALAGI"
BASE COUNT          440 a          963 c          957 g          397 t
ORIGIN      
        1 cgaagctgtc gatgacactc gtgcggcagt gacgggcgcc gcgcctcacg tcccgccgtc
       61 cagggaggcc ttggcctcgc gccaggtctt ccggaccgtt gcgccgagaa tgcacgcgcc
      121 tccaagcagc ggccagaaac ccggcgcctc gcggtgaaga agccacgccc ataaggggct
      181 gagcgccggc tccagcagca gcaacagcga cgcgtcgaca gccggcacgt gccggatggc
      241 ccgcgtcagc agcacgtagg ccaggcccac ctggaagagc ccgagatacg cgatgccggc
      301 ggcgtcgcgc ggcgcgacgg cgtgcagcgg gagcgcaagg ggcagggtgg ccaggcaggc
      361 gatgatattg ccggtgagcg ccgcgctgcc ggctgcgatg cctcccgcgt gcgggtctcg
      421 tttcgcgatc cagcggagcc ccatcaccgt caggctccag cagaccccac tcacgaggcc
      481 cacggcgttc ccaagcgcgc ggttcggagc cgtcaccgtg ggcgcgccgg aaccggcgac
      541 gaggagggcc atgccggccg cgatggccgc cagcacgggc aggtcccggc ggcggaggtg
      601 ctcgccgagc agccacgggg cgaggagcgc aatgtagagc ggcgccgcgg cctgcaggaa
      661 cacggcgttg gccgccgtcg tgagcgtgtt ggccgtcacg aaggtgacga gcgtcgcggc
      721 gtaggccgcg ccgacgagcg cggtgggtgc ggtcagccgg ggcagtttcc tgaccgccag
      781 gagcaggacg gctgccgcga cccccgaccg caggctcgca atctgccagc tcgtcagcga
      841 caccgccttg atggcggccc cgccggtcga gaagaggagc gccgtcagca ggatggccgc
      901 gcgggccggc acgctcggca cccgctcatc ctacaccagc cggcgccggg cttcgtgacg
      961 ccgccccgta cacagcgcgg gggacctcgc ggtcccccgt gccgagccgc gcagcccggc
     1021 cgcggctagc cgtgcagcgc gtgatacttc gcctcgagct gctccctggt ctccacgtag
     1081 ttcggatcct gggtgatgca tccgtccacc gggcagacat cgacgcactt cgacgtgtcg
     1141 tacgcgccga cgcattccgt gcacttcgcc ggatcgatca catagatcga atcgccctgc
     1201 gtgatcgcct cattcgggca ttccggctcg cacgcgccgc agctgatgca atcctgatta
     1261 atccacaatg ccatgactcg cagcctccag ttgaccgaga acgaaccgaa acgcagttcc
     1321 gtgttctctg tataacaaac tccgatcagc ccttcaagac tcaattccgg tcgcacatcg
     1381 ccggtctcgc ggggcgctgc gggaggtcgg ccgaacggcg accgccgccg gcctatatgc
     1441 ccgccagcgc ctgacgcaga tccgcgatga ggtcctccgg gtgttccagc cccactgaca
     1501 gccgcaccat cgacggcgtg atgcccatct cgagctggcg ccccggcggg atgtccgagt
     1561 gcgtcatcgt cgccgggtgc tcgatgaggc tctccgtgcc ccccaggctg accgcgagct
     1621 tgatcacctc gagccggttg agcagccgga acgcctcggc ttcgccgcca tcgacctcga
     1681 acgaaaacgt ggatcccggc gcctcgcact gccgctggta gagtccgagt tgggggtcgg
     1741 cgggccccag aaagccgagg taatggacgg ctttcacctt cgggtgcccg gcgaggaact
     1801 cggcgacgcg cctggcgttc tccatcgagc ccgtcatgcg gagcttcagg gtttccaggc
     1861 tgcggagcag cagccagcag gtgtgagggt cggcaatcgt gccgagaatc gtgcgcaggc
     1921 ctcgaacggg cgcgatggcc gattcggtcc ccaggcaggc gcccgcaatg aggtcgctgt
     1981 gcccgccgac gtacttggtg agcgagtaga tcacgaagtc ggcgccgtgg cgcagggggc
     2041 gctgccacag cgggccgagg aaggtgttgt ccaccgctac gagggggcgg cggccatcgg
     2101 cgttccgcag cgagcgggcc gcagccgcgc acgcggcgat gtccacgagt ccgttcgtcg
     2161 ggttcgcggg ggtctccacg tagatcaacc cgacgcggcc aaggccacgc gccgtctcga
     2221 cggcgtcggc gaggccgggc tcgagcgagc cggcgccgaa ccagacacgc ttgatcccga
     2281 actgcggcag gatgttctcg atcaggaatt ccgtgccgcc gtagacgggc gcgctggcaa
     2341 gcaggacgtc tccgggcctg agcacgctga acatggtcgt cgagatcgcg gccatccccg
     2401 acgagaacgc caggccggat tccgcctcgt cccagaggcc gaggcgctcc tcgaggatct
     2461 cgaggtcggg attgttcacg cgcgagtaga tcagccccgg ctcctcggcc ggcccctgct
     2521 cgcgcagccc gtacgccagc tggaagaacg acttgccgtc ctcggccgtc ttgaaggtga
     2581 acgtcgaggt ctggaagatg gggcacttga cggcgccctc ggagaactgc gggtcgtacc
     2641 cgtagcccat catcagcgtc tcgggccgga gcggatggcc ctgcacgtcc cgatgcctgt
     2701 acttcctggg cgggtgaaac atggcgcatc cctcgtcgtc gcgtcgcgcg gccccgc
//
LOCUS       DAOSIF010000421         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_32147_length_2739_cov_2.952683, whole genome shotgun sequence.
ACCESSION   DAOSIF010000421 DAOSIF010000000
VERSION     DAOSIF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32147_length_2739_cov_2.952683"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLT35_11995"
     CDS             complement(<1..606)
                     /locus_tag="PLT35_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG29992.1"
                     /translation="MPDLPKVFDHKDVDARWDAFWESIGAFTARPDPAKPVFSMVLPP
                     PNVTGSLHMGHALNHTLPDILARWKRMLGHDVLWLPGTDHAGIATQNVVEKQLALEGK
                     TRHDLGREAFEARVWEWVKRSHATITGQMRKLGESVDWSRERFTLDPGLSRAVRREFV
                     SLYREGLIYRDAYIVNWCPRCRTALSDLEVQHKEADGKLYYV"
     gene            complement(727..1890)
                     /locus_tag="PLT35_12000"
     CDS             complement(727..1890)
                     /locus_tag="PLT35_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG29993.1"
                     /translation="MPHKLLLADDSVTIQRVIELTFADEDVQVIAVGDGKQAIERIEA
                     ERPDIVLADVGMPERDGYDVAEFVKTSPHLAHIPVLLLTGAFEPVDEGRASAVGCDGV
                     LAKPFEPQMVITRVKELLAGVPPSVAGGPVGPREAGGTAGAEDRGAAPAAPADAGDTI
                     QAEAPLAPPPGLPPEGPARTDDPLAAAKLRLSRRTPIAVPGFGAPVSPAASTTDLDEY
                     FDQLDAAFTSLQAPGQTAAADVPPAPVREHAAADSAAAAGPEPERAAPPGAAAPAPVA
                     ETPAETPAAAASGAPSAAAPVAAPPPAPGISQAFSALFAAERGETPEAAPLFIGPGTL
                     PPAVMEDLADRVTRQVLERMSDRLVRETVQDAVSRVAERLVRDEIERVKATLK"
     gene            2154..>2739
                     /locus_tag="PLT35_12005"
     CDS             2154..>2739
                     /locus_tag="PLT35_12005"
                     /inference="COORDINATES: protein motif:HMM:NF014827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOG29994.1"
                     /translation="MRGSAFGSLLEDFDRAARLLDLDPGLRQILARPKRQIVVSCPVE
                     MDNGDVEVFSGYRVQYNIALGPALGGIRLHPGVTLDDMTARAARMTWRCAVAQVPFGG
                     SQGGLVCDPSRMSRREIERLTRRYVAEIADAIGPDRDIAAPGLNADGQVMAWVMDTYS
                     MRMATMATAVAVGKPLEIGGLAGRPGAAGRGVAIA"
BASE COUNT          350 a          996 c          979 g          414 t
ORIGIN      
        1 cacgtagtag agcttcccgt cggcctcctt gtgctgcact tccaggtccg agagcgccgt
       61 gcggcacctg gggcaccagt tgacgatgta ggcgtcgcgg tagatcagcc cctcgcgata
      121 gagcgacacg aactcgcggc ggacggctcg gctcaggccc ggatccagcg tgaagcgctc
      181 ccgcgaccag tccaccgact cgccgagttt ccgcatctgg ccggtgatcg tcgcgtggga
      241 gcgcttcacc cactcccaca cgcgggcctc gaacgcctcc cggccaaggt cgtgccgggt
      301 cttcccctcg agtgcgagct gcttctcgac gacgttctgc gtggcgatcc cggcatggtc
      361 cgtgccgggg agccagagca cgtcgtggcc cagcatgcgc ttccaccgcg cgaggatgtc
      421 gggcagcgtg tggttgagcg catggcccat gtgcagcgaa cccgtgacgt tcggcggcgg
      481 cagcaccatg ctgaacaccg gcttggccgg gtcgggcctg gctgtgaagg cgccgatgga
      541 ttcccagaac gcgtcccagc gcgcgtcgac gtccttgtgg tcgaagactt tcggcaggtc
      601 aggcatgcgg tccttcaaaa cctcgattgt acaacgttcg gccgggccgg cttcagcctc
      661 gccggccgcc gggcggacgg ggtgcgggcc ggcgcgcgcc agccggagcc ggcggccgcg
      721 agacgcctac ttgagcgtgg ccttgacgcg ttcgatctcg tcgcggacga ggcgttccgc
      781 tacccgcgac accgcgtcct gcacggtttc gcgaacgagc cgatcgctca tccgctcgag
      841 tacctggcgc gtgacgcgat cggccagatc ctccatgacg gcgggcggca gcgtgccggg
      901 accgatgaag agcggcgccg cctcgggcgt ctcaccgcgt tcggcggcaa acagcgccga
      961 gaacgcctgg gagatgccgg gcgccggcgg cggcgcggca acgggcgccg cggcggatgg
     1021 cgcgccggag gcggccgccg cgggtgtctc cgcgggtgtc tccgcgaccg gggccggcgc
     1081 ggcggcccct ggtggcgcgg ctcgttcggg ctcggggccg gccgcggccg cggaatccgc
     1141 ggcggcgtgc tcccgcaccg gagccggtgg cacgtcggcc gccgccgtct gccccggtgc
     1201 ctgcaggctg gtgaaggccg cgtccaactg gtcgaaatac tcgtcgagat ccgtcgtcga
     1261 cgccgccggc gacaccggcg cgccgaaccc cggcaccgcg atcggcgtcc gccggctgag
     1321 gcggagcttc gccgcggcca gcggatcgtc ggtccgggcc ggcccttcgg gcggcaggcc
     1381 gggtggaggc gccagcggag cctctgcctg gatggtgtct cccgcgtccg ccggagcagc
     1441 cggggcggcg ccccgatcct ccgcgccggc ggtcccgccc gcctcacgcg gcccgaccgg
     1501 cccgcccgcc accgaaggcg gcacgcctgc cagcagctcc ttcacgcgcg tgatcaccat
     1561 ctgcggctcg aacggcttgg ccagcacgcc gtcgcatccg accgcgctcg cccgtccttc
     1621 gtccaccggc tcgaacgcgc ccgtcaggag cagcacggga atgtgcgcca ggtgggggct
     1681 cgtcttcacg aactccgcga cgtcgtaccc gtctcgttcc ggcattccga cgtcggccag
     1741 gacgatgtcc ggacgctcgg cctcgatgcg ctcgatcgcc tgcttcccgt cgccgaccgc
     1801 gataacctgg acgtcttcgt cggcgaacgt cagttcgatg acgcgctgga tcgtgacgct
     1861 gtcgtcagcg agcaagagct tgtgcggcat ggccacaact acctggtgag tcgccgatgg
     1921 gatgaccggc tgccaaccgg agaagcgtag cggccgctcc cggcgaagtc aaccggcccg
     1981 ccgtcggccg gccggctgtc cgccttgact tgtccgccgc cgtgtaggta cgctgacgcc
     2041 tgcccgcccg gggcctcgac acgcgccttc cgttccccgc gtgtgaacct ggctggatgg
     2101 tggctccctt gaacgacaac gcttccagcg ttcctcgcgg ctcggctcct tccgtgcgtg
     2161 gcagcgcctt cggttccctg ctggaggact tcgaccgcgc cgcccggctc ctcgacctcg
     2221 accccggcct ccgccagatc ctggcgcggc ccaagcgcca gatcgtcgtg tcgtgtccgg
     2281 tcgagatgga caacggcgac gtcgaggtgt tctcggggta ccgggtccag tacaacatcg
     2341 ccctcggccc cgccctcgga ggcatccggc tccacccggg cgtgacgctg gacgacatga
     2401 ctgcgcgggc ggcccggatg acgtggaggt gcgccgtcgc gcaggtgccc ttcggcggga
     2461 gccagggcgg cctggtctgc gatccgtccc ggatgtcgcg ccgggagatc gagcgcctga
     2521 ccaggcgcta cgtggcggag attgcggatg cgatcggccc cgacagggat atcgctgcgc
     2581 cgggtctgaa cgccgacggc caggtgatgg cctgggtgat ggacacctac agcatgcgca
     2641 tggccacgat ggccacggcc gtcgccgtcg gcaagccgct ggagattggc gggcttgccg
     2701 gccgccccgg cgcggcgggc cgcggcgtcg cgatcgctg
//
LOCUS       DAOSIF010000422         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_32499_length_2717_cov_4.531180, whole genome shotgun sequence.
ACCESSION   DAOSIF010000422 DAOSIF010000000
VERSION     DAOSIF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32499_length_2717_cov_4.531180"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..544)
                     /locus_tag="PLT35_12010"
     CDS             complement(152..544)
                     /locus_tag="PLT35_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOG29995.1"
                     /translation="MVGTRLGPYGIVGLLGAGGMGEVYRARDPRLDRDVALKVLSSGS
                     PADRTARARLVREARKAARLSHPNVCTIYEVGEADGHVYIAMELVQGRPLDEVLAEPP
                     PPGTRSATGRRSRALWPTPTRAGSYTAT"
     gene            666..1841
                     /locus_tag="PLT35_12015"
     CDS             666..1841
                     /locus_tag="PLT35_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29996.1"
                     /translation="MAVADEQQRDRLLRRRDRRGHRHGSSARTMTDRRSACIIGSSFL
                     DGTRRRSGPEGHPSGARAPRHAVRRASTLKEETAGAGSSNLAKALLHLSPQGGTMKAS
                     TPVILFGLLAGATSAMAQAPAVQAPAEQAPSGTLRREVAAMVGASANPEGAIARVEVR
                     WRRPLSRSTAPALSDAHLSGGVSPEITPAYTRLGVWAEFAPVSFLGVRAGFEPALYFG
                     TFRSLLSFSDIRAPFNRAALKARRDEATAGSALRVFVRPVLRMKVGRVGAEVTAQVER
                     WRSSAAGPFYYEPSRNALVAAGGGNVGVLSAAVLYDASARLGVGAMYDLTHVYAASAN
                     RIQRAGALVTFAPRKGFPVLGRPSLTAVAGVYLQDPCRPNQVFAGLGLDFVLRKPAE"
     gene            complement(2506..>2717)
                     /locus_tag="PLT35_12020"
     CDS             complement(2506..>2717)
                     /locus_tag="PLT35_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG29997.1"
                     /translation="LVETGGVAGGRPAMSRRPEAWSRLLADGGRLLRAGVVTTREVHA
                     QGLDAHVMLTPGSPERRIGGRDARI"
BASE COUNT          385 a         1000 c          918 g          414 t
ORIGIN      
        1 tttcccgtgg caagaccgct aatggtgccc gtgttcgatg tcagggtgac cgtctcggtg
       61 cccgcgggga cctcgccggc caggggtttc gccaggccga aatccagcac cttcgcctgg
      121 ccctccgggg tgatgacgac gttggcgctc ttcaggtcgc ggtgtacgac cccgcgcgcg
      181 tgggcgtggg ccagagcgct cgcgatctgc gtcccgtagc ggagcgcgtc cccgggggcg
      241 gcggttcggc caggacctcg tccagcggcc ggccctgcac cagctccatc gcgatgtaga
      301 cgtggccgtc cgcctcgccc acctcgtaga tcgtgcagac attcgggtga ctgagccggg
      361 cggctttgcg cgcctcgcgc acgaggcgtg cccgcgccgt tcggtcggcc ggactgccgg
      421 aggacagcac tttgagggcg acatcgcgat cgagccgcgg gtcgcgcgcc cggtacacct
      481 cccccatgcc gccagccccg agcagaccca cgattccgta cgggccgagc cgcgtgccca
      541 ccatgctcgt cctccgcgga cgacgggcgc ccaccgggcg tgtcggccgt ggcgcccgcc
      601 ttggccagcc cgccgagggt gccgtcgagg gtcgcgtcct tgggtcccca ggcggcgagg
      661 tgcgaatggc tgtcgctgac gaacagcagc gtgaccggct gctgcggcgc cgcgaccgcc
      721 gtggacatcg ccacggcagc agcgccagga cgatgacaga ccgccgcagt gcgtgcataa
      781 tagggtcctc cttccttgac ggcacccgcc ggaggtcggg tcccgagggg catccgtcgg
      841 gcgcccgggc gccacgacat gccgtccggc gtgcctcaac gctgaaagaa gaaaccgcgg
      901 gagcaggttc cagtaacctc gcgaaggcct tgcttcatct ctctccgcag gggggaacca
      961 tgaaggcctc gacgcccgtg atcctgtttg gcctgctagc gggcgctacc tcggccatgg
     1021 cgcaggcacc ggccgtgcag gcgccggctg aacaggctcc ttccgggacg cttcgccgag
     1081 aggtcgcggc gatggtgggc gcatccgcga atccggaggg ggccatagcg cgcgtcgagg
     1141 tccgctggcg acggccgctc tcgcgctcga cggcgccggc gctgtcggac gcccacctct
     1201 ccggtggcgt cagccccgag atcacccctg cctacaccag gctcggcgtc tgggccgagt
     1261 tcgcgcctgt ctcgttcctc ggcgtccggg cgggcttcga gccggcgctc tatttcggga
     1321 cgttcagatc cctgctcagc ttctccgaca tccgcgcgcc attcaatcga gcggcgctga
     1381 aggcacgccg cgacgaggca accgccggca gtgccctgag ggtattcgtg cgcccagtcc
     1441 tccggatgaa ggtcgggcgt gtgggcgccg aagtgacggc acaggtcgaa cgatggaggt
     1501 ccagcgccgc gggaccgttc tactacgagc ccagccgcaa cgcgctcgtc gccgcggggg
     1561 gcggcaacgt gggcgtcctg tcggcggccg tcctctatga cgcgtcggcg aggctcggag
     1621 tcggagccat gtacgacctc acccacgtgt acgcggcgtc agcgaaccgg attcaaaggg
     1681 ccggagcgct ggtcaccttc gcgccccgaa aagggtttcc ggtcttgggc cgtccctcgc
     1741 tcacggcggt cgccggcgtc taccttcagg atccctgccg cccgaaccag gtgttcgccg
     1801 gccttggtct cgacttcgtc ctgcggaaac cggcggagta aagcgtcacg gcgccggcgg
     1861 aagcaggtcg agttccagca gaagccagcg tcgttccgcg tgtggagcgg gaggccagtc
     1921 tgtcacgagc cacgcccggc cgtcctcggt cagaccactt ggccacctcg agcctgacca
     1981 gccggcccga cacggcatcc tcggcgatgc aggccacgat cgtctcgctg tcgtccatca
     2041 gcgcacggac ggcgtaccga tcctcgccgc tcgaggcgcc gtcctgaccc gcgcccgtcc
     2101 ccgcctccgg gagggcgagc cgcagcaggc acgccgggca cagaggctcg tcgacgaacg
     2161 ccgttcccgg actcccgcac gagggacaag ttgaacgtcg gcacggacgc ggatccctcg
     2221 ccgatcgaga cgtcctttcc cgcgcgcttg agcaactgcg ccagagccct gacgacggcg
     2281 ggcttcgtcg tcgccttcgg gctggaggtc accaggttcg gcttgagcat cacccggttc
     2341 ttgcccctgg cgatcgcccg catccttccg agcaaatcga tggcttcctc caccgcgcca
     2401 ccgaccttcc cgttccggac gcgcacgacg ctcaccaccg tcttgggccc ggctcacagg
     2461 acggcgggga cgagggcgcc agccaggaag tcccgacgac cgagctcaga tcctggcatc
     2521 tcgccctcca atgcggcgtt ccggcgatcc cggggtgagc atcacgtgcg cgtccagacc
     2581 ctgggcgtga acctcccgcg tggtcacgac gccggctctc aagagccggc ctccatccgc
     2641 aagcagccgg gaccacgcct ctggccggcg gctcatcgcg ggcctcccgc cagccactcc
     2701 cccagtttcg accagcg
//
LOCUS       DAOSIF010000423         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_32580_length_2712_cov_4.106135, whole genome shotgun sequence.
ACCESSION   DAOSIF010000423 DAOSIF010000000
VERSION     DAOSIF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32580_length_2712_cov_4.106135"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="PLT35_12025"
     CDS             complement(<1..1528)
                     /locus_tag="PLT35_12025"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOG29998.1"
                     /translation="MPEPLRSPARPRRRWLRRLLLTVLVAIAASAALLYWRLRAPLPD
                     TCGSVKVDGVSAPVEIVRDIDDVAHIRANSQADALFGLGYVHAQERLWQMEFQRRVAQ
                     GRLAEIAGPEALPADRLLRTVGLARAARDTWPRLDGRVRALVEAYVAGVNAFLERHRG
                     GGLPIEFAIFRVSPDPWTGEDVVAWQKTMGWLLSANWLEESFRAALATRVGDEGASVL
                     MPAYTPGGPIILPPDALLPSRPLPGAGASAPGPAGALTTRAPGFASASARTVGAAAAA
                     DLAALAAIAGRVAVAPPIGGGSNNWVVSGSRSVTGKPLLANDPHLAGQTPAVWYLAHV
                     TGGGYDAIGATLPGMPGIVLGHNGRIAWGVTALLGDVQDLYIERITARDQAEYQGALE
                     RIRVVREVIRVRGQADVPIRIRITRHGPVVSDALERPQSTIALRWTGLDAEDHTLESF
                     IPLGSAGGWSEFRSALRGMHVPLLNFVYADVDGNIGYLGPGAIPVRTSGDGARPVAGW
                     T"
     gene            complement(1551..2642)
                     /locus_tag="PLT35_12030"
     CDS             complement(1551..2642)
                     /locus_tag="PLT35_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOG29999.1"
                     /translation="MATRTVMTSRERVLAAMRRQETDRPPFDLSFGIATAQLASIRAR
                     TGADDPWDYFGADTRMIWPGPTRLDTDFSRYLGRLPRGSTVDEWGLGHVPTASTDADH
                     AHLEGFRHPLEEAESLADIAEYPLPDVDAAYRYESVRAEVEAVHRRGLAAMAMLECTI
                     FERAWYLRGMERLLLDFSDGSAMATVLLDRITALRIGQARHYAASGADVICYGDDVGT
                     QRGLLMSAAMWRTWLKPRLAATIAAARDVRPDVLVFYHSDGNVEALIPDLIDIGVDIL
                     NPVQPECLDAAALKRQYGSRLSFWGTIGTQSTLPFGSPDDVQREVRTRIETVGVGGGL
                     FLAPTHVIEPEVPFENLVAFVEAVTGERG"
BASE COUNT          376 a          990 c          988 g          358 t
ORIGIN      
        1 ccgtccagcc cgcaaccggc cgcgcgccgt ccccgctcgt ccgcactgga atcgcgcctg
       61 gccccaggta gccgatgttg ccgtcgacgt cggcgtagac gaagttcagc agcgggacgt
      121 gcatgccgcg gagcgccgag cggaactcgg accagccgcc ggccgagccg agcgggatga
      181 agctctcgag cgtatggtcc tcggcgtcca gcccggtcca gcgcagcgcg atcgtggact
      241 gcggacgctc gagcgcgtcg gaaacgaccg gtccgtgacg cgtgatgcgg atgcggatgg
      301 gcacgtccgc ctgcccgcgc acacggatga cctcccggac cacgcggatg cgctcgagcg
      361 cgccctggta ctcggcctgg tctcgggcgg tgatccgctc gatgtagagg tcctgcacgt
      421 cgcccagcag cgccgtcacg ccccacgcga tgcgcccgtt gtggccgagc acgatgcccg
      481 gcatgccggg cagcgtcgcg ccgatggcgt cgtagccccc gccggtcacg tgcgcgagat
      541 accagacggc cggcgtctgt cccgccaggt gcgggtcgtt cgccagcagg ggcttgccgg
      601 tcaccgaccg cgagccggac acgacccagt tgttgctccc gccgccgatg ggcggggcga
      661 cggcgaccct tcccgcgatc gccgccaggg ccgccagatc cgctgcggcc gctgcgccga
      721 cggttcgcgc gctcgcgctc gcaaaccccg gcgccctggt cgtcagcgcg cctgccggcc
      781 ccggcgcgga ggcgccggct ccgggcagcg gtctcgaggg cagcagggcg tccggcggca
      841 ggatgatggg gcctccgggg gtgtaggccg gcatcaacac gctcgcgccc tcatcgccga
      901 cgcgcgtcgc cagcgccgcg cggaacgact cctcgagcca gttcgcgctc agcagccagc
      961 ccatggtctt ctgccaggcc acgacgtcct cgcccgtcca cgggtccggg gacacgcgga
     1021 agatcgcgaa ctcgatggga agccccccgc cccgatggcg ctccaggaag gcgttgacgc
     1081 cggccacgta ggcctccacc agcgcgcgaa cgcggccgtc gagcctgggc caggtgtcgc
     1141 gggccgcgcg cgccagcccg accgtccgca gcagcctgtc ggcgggcagg gcttcggggc
     1201 cggcgatctc cgccagacgg ccctgcgcga cccgccgctg gaactccatc tgccacagcc
     1261 gctcctgcgc gtgcacgtag ccaaggccga agagcgcgtc ggcctggctg tttgcccgga
     1321 tgtgcgccac atcgtcaatg tcgcggacga tctcgaccgg cgccgacacg ccgtctacct
     1381 tgacgctgcc gcaagtgtcc ggcagcggcg cacgcaggcg ccagtacagc agggcggcag
     1441 acgccgcgat cgcgacgagc accgtgagaa gcagccgccg gagccagcgg cgccggggcc
     1501 gggcgggaga gcggagcggt tcgggcatgg ccatccgggg cggcctcatc ttacccgcgt
     1561 tcgccagtca ccgcctcgac aaacgccacg aggttctcga agggcacctc cggctcgatg
     1621 acgtgggtcg gcgcgaggaa cagcccgccg ccgacgccga ccgtctcgat gcgcgtccgg
     1681 acctcccgct gcacgtcgtc cgggctgccg aacgggagcg tggactgcgt gccgatggtg
     1741 ccccagaagg aaagccggct gccgtactgc cgcttgagcg cggcggcatc caggcattcc
     1801 ggctgcacgg ggttcaggat gtcgactccg atgtcgatga gatcggggat gagggcctcc
     1861 acgttgccgt cgctgtggta gaagacgagc acgtccggcc ggacgtcgcg cgccgcggcg
     1921 atggttgcgg ccaggcgcgg tttcagccac gtgcgccaca tggccgcgct catgaggagg
     1981 cctcgctgcg tgccgacgtc gtcgccgtag caaatgacgt cggcgcccga ggccgcgtag
     2041 tgcctggcct gcccgatgcg cagagcggtg atgcggtcga gcagcaccgt cgccatggcg
     2101 cttccgtccg agaagtcgag caggaggcgc tccatgccgc gcaggtacca cgcccgctcg
     2161 aaaatcgtgc actccagcat ggccatggcc gcgaggcccc gccggtgaac cgcctcgact
     2221 tcggcacgca cgctctcgta gcggtaggcg gcgtccacgt cgggcagcgg gtactccgcg
     2281 atgtcggcga gcgactccgc ctcctccagc gggtggcgga acccctcgag gtgggcgtgg
     2341 tcggcgtcgg tgctcgcggt cggcacgtgc cccaggcccc attcgtccac tgtcgagcct
     2401 cgaggcaggc gcccgagata cctggagaag tcggtgtcga gccgggtggg gcccggccag
     2461 atcatgcgcg tgtcggcgcc gaagtagtcc caggggtcgt ccgcccccgt gcgggccctg
     2521 atcgacgcaa gctgcgccgt cgcgatgccg aaggacaggt cgaacggcgg gcggtcggtc
     2581 tcctgccgcc gcatcgcggc gaggacgcgc tcccggctcg tcatgacggt gcgagtcgcc
     2641 acgcggggac ctcaggcgcc gggcgccatc aggcggcggg ccagtgcgag ggccgcggcg
     2701 gcgttgtccg cg
//
LOCUS       DAOSIF010000424         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_32733_length_2701_cov_3.010960, whole genome shotgun sequence.
ACCESSION   DAOSIF010000424 DAOSIF010000000
VERSION     DAOSIF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32733_length_2701_cov_3.010960"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /gene="glgP"
                     /locus_tag="PLT35_12035"
     CDS             complement(<1..1217)
                     /gene="glgP"
                     /locus_tag="PLT35_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOG30000.1"
                     /translation="MTGSIAPAGLAARALPDPLAPLAELALDLHWSWNRAGDAVWRRL
                     DGPLWERTENPWLLLQHVSRERLDQLAADATFLRELEALRAARARYRASKPWRPAGSP
                     DLPRVAYFSMEFGLHEALPLYSGGLGVLAGDYLKTASDLGLPIVGIGILWQQGYFRQV
                     LDAEGRQTELYPYNEPASLPLQPVAGPGGERLRVALDFPGRTILLRASQVTLGRTTLY
                     LMDSNDPCNAPADRGLTSALYGGGSETRFLQEIILGVGGWRLMRALGLSVEVCHLNEG
                     HAAFAVIERARDFMEAHGTTFGEALWATRAGNVFTTHTAVPAGFDTFPVGEMDAYRPY
                     LDAYVRSLGLSWTELLGLGRRNADDPNEPFNMAWLAVRGSGWVNGVSRLHGAVSRRMF
                     AGLFPRWPEAEVPV"
     gene            1418..>2701
                     /locus_tag="PLT35_12040"
     CDS             1418..>2701
                     /locus_tag="PLT35_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG30001.1"
                     /translation="MTAPSATPRPNAAREQPAADGSAPPPPKPWRTEGLPAGKAPEKR
                     PRWVTAAVWMAVYLAFFGVLTLQDRVSGPQAIPYTEFKFQVAAKNVGEVFARGDSIQG
                     ALKKAAPRPGEQGGTYQQFTTERPTFAGDDLLEELAGGGATVRATPIVQHRGFLANLL
                     ISSAPLLLLVAFYVWMFRRQQGAMAGGLFGGRKQTRVDPETIRVTFDDVAGIDEVEAE
                     INEVVDFLRDPGKYQRLGARAPKGVLLAGAPGTGKTLLARATAGEAKVPFFSASASEF
                     IEMIVGVGASRVRELFAEARKVAPAIVFIDEIDTIGRARGGARAIGGHDEREQTLNQI
                     LTEMDGFSGHEGVVVLAATNRPDVLDPALLRPGRFDRQVIVHAPDHKGRVQILQVHTR
                     KVPLSGDVDLEQLASATPGMTGADLANLVNEAALRA"
BASE COUNT          410 a          942 c          981 g          368 t
ORIGIN      
        1 acagggacct ccgcctccgg ccatcggggg aacagcccgg cgaacatccg ccggctgacg
       61 gcgccgtgca gacggctcac cccgttgacc cagcctgagc cgcgcacggc gagccacgcc
      121 atgttgaacg gctcgttcgg gtcgtccgcg ttccgccggc cgagccccag gagctccgtc
      181 cacgacaagc cgaggctccg cacgtacgcg tcgagatacg ggcggtaggc gtccatctcg
      241 ccgaccggga aggtgtcgaa gcccgccggc acggcggtgt gcgtcgtgaa gacgttgcca
      301 gcccgcgtcg cccacagagc ttcgccgaag gtcgttccgt gcgcctccat gaagtcccgc
      361 gcgcgctcga tgacggcaaa cgccgcgtgt ccctcgttga ggtgacagac ctcgacgctc
      421 aggccaagcg ctcgcatgag gcgccatcct ccgacgccga ggatgatctc ctgcaggaag
      481 cgcgtttcgg atccgccgcc gtacagtgcg ctcgtgaggc cgcggtcggc aggcgcgttg
      541 cacgggtcgt tgctgtccat gaggtacagc gtcgtccggc cgagcgtcac ctgcgaggcg
      601 cgcagcagaa tcgtgcgccc gggaaagtcg agggcgaccc ggaggcgctc gccgccaggg
      661 ccggcgacgg gctgcagcgg caggctcgcc ggctcgttgt agggatacag ctcggtctgc
      721 cggccctcgg cgtcgagcac ctgccggaag tagccctgct gccagaggat tccaatcccg
      781 acgatcggca ggcccagatc gctggctgtc ttcaggtagt cgcccgcgag tacgccgagg
      841 ccgccggagt agagcggcag cgcttcgtgc aggccgaatt ccatgctgaa gtacgccact
      901 cggggcaggt ccggcgagcc ggccgggcgc cagggcttcg acgcgcggta gcgcgcccgc
      961 gctgctcgca gggcctccag ttcgcgcagg aaggtcgcgt cggcggcgag ctggtcaagg
     1021 cgttcgcggc tcacgtgctg caggagcagc caggggttct cggtgcgttc ccagagcggg
     1081 ccgtcgaggc gccgccagac tgcgtccccg gcgcggttcc acgaccagtg caggtcgagc
     1141 gcaagctcgg ccagcggcgc cagggggtcc gggagcgcac gggccgccag gcccgcgggt
     1201 gcgatcgatc cggtcatgac ccgagtcctc ccgaggggca cggagcgggg ctccggaacc
     1261 cgcatgctat gatcctagtc gcctcgacag gcgtgccggc gcgggcggtg ctgccggcgc
     1321 ggcacgccgg gtatcgtcgc cgattgttgc accttatcac tgcgccccag cgtgcgcgca
     1381 actcacgcgc cgcccggcga gaggaaggcg ccgaacgatg accgcaccga gcgcgacacc
     1441 ccgccccaac gcggcgcgcg agcagccggc cgctgacggg tccgcgccgc cgccgccgaa
     1501 gccgtggcgg accgagggac tgccggccgg gaaggcgccg gagaagcgcc cgcggtgggt
     1561 cacggccgcc gtctggatgg ctgtctacct ggccttcttc ggcgtgctga cgctgcagga
     1621 ccgggtgtcc ggtccgcagg ccattcctta cacggagttc aagttccagg tcgccgccaa
     1681 gaacgtcggc gaggttttcg cgcgaggcga ctccatccaa ggcgcgctga agaaagcggc
     1741 gccgcgcccg ggtgagcagg gcggaacgta ccagcaattc acgaccgagc gacccacttt
     1801 cgccggcgac gatctgctcg aggagctcgc gggagggggc gccacggttc gggccacgcc
     1861 gatcgtgcag catcggggat tcctggcgaa cctcctcatc tcctccgccc cgctgctgct
     1921 gctggtcgct ttctacgtgt ggatgttccg gcgccagcag ggcgcgatgg ccggcggcct
     1981 gttcgggggc aggaagcaga cccgggtgga tccggagacg atccgggtga ccttcgacga
     2041 tgtggccggc atcgacgaag tggaagcgga gatcaacgag gtcgtggact tcttgaggga
     2101 cccgggcaag taccagcgcc tcggggcccg cgcccccaag ggcgtcctcc tggcaggcgc
     2161 gccgggaacg ggcaagacgc tgctggcgcg cgccacggcc ggcgaggcga aggtgccgtt
     2221 cttcagcgcc agcgcctcgg agttcatcga gatgatcgtc ggcgtcgggg cgagccgcgt
     2281 ccgcgagctg ttcgccgagg cccgcaaggt cgctcccgcc attgtcttca tcgacgagat
     2341 cgacaccatc gggcgcgcgc gcggcggcgc gagggccatc ggcggccacg acgagcgcga
     2401 acagacgctg aaccagatcc tgaccgagat ggacggcttc tccgggcacg aaggcgtcgt
     2461 cgtgctggcc gcgacgaacc gcccggacgt gctggatccc gcgctcctga gaccgggacg
     2521 cttcgaccgc caggtcatcg tgcacgcgcc ggaccacaag gggcgcgtcc agatcctgca
     2581 ggtccacacg cgcaaagtgc cgctctccgg cgacgtggac ctcgagcagc tggcgtcggc
     2641 gacgccgggc atgacgggcg cggatctcgc caacctcgtc aacgaggccg cgctgcgggc
     2701 g
//
LOCUS       DAOSIF010000425         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_32995_length_2685_cov_3.688593, whole genome shotgun sequence.
ACCESSION   DAOSIF010000425 DAOSIF010000000
VERSION     DAOSIF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32995_length_2685_cov_3.688593"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..835)
                     /locus_tag="PLT35_12045"
     CDS             complement(77..835)
                     /locus_tag="PLT35_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG30002.1"
                     /translation="MAHDPYRDLADYYDLMASDPGIQALYREWRELLLAAIRKRGLRP
                     RVLVDLACGTGNSTIPWTRRRGWTVVGVDQSAAMLRAARRKSREVRWVRQDLRALRLT
                     ERADLVTCHFDALNHVLREAELQRVFDGVGRILREGGLFQFDLNTAHWLRRLSASQKL
                     FRLGPHAFTAYNAFDPRTGVALLRQLWFVKRGRHYRKLEVAVRERAYDRAAVRRMLRQ
                     SGLRLVTVRAQRALGAAPIRDYFLAVRLPARPAR"
     gene            1016..1948
                     /locus_tag="PLT35_12050"
     CDS             1016..1948
                     /locus_tag="PLT35_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOG30003.1"
                     /translation="MKRRDFIARGLASAAALAAAPLPALARQQAGPLAPPGPPKPKFR
                     LRYAPSFGQFTSHAGAGLAAQVEFASNEGFTAMFDNGLMSRPLADQETIVRELEKRGM
                     TLGPFVLLADFKNRNFVLRDKDVRAALLQKMRDGVETMKRTGARTALVVPGRYDESLA
                     WEYQTANLVDNLRACMDVCAPAGLTIVLEPLNPRDHPGLFLSKIPQAYQICRAVASPH
                     CKIVNDIYHQQISEGNILPNIEAAWDEIAAFHVGDNPGRKEPTSGEINFRNVFKYLHG
                     RGYQGPICMEHGQSKPGLEGERAVIDAYRSCDAF"
     gene            1962..>2685
                     /locus_tag="PLT35_12055"
     CDS             1962..>2685
                     /locus_tag="PLT35_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG30004.1"
                     /translation="MSQDQHTTVTRRDFVKTAAAASMAAAIPGGVGVFAQGSDAIRVG
                     VIGCGGRGTGAAIDCLKADPAVEIVALGDLVPDRVESALARLTKEYASRVRVPASRQF
                     VGFDNYKGVCSVPEVNLIVTAAPPGFRPIHLKAAIEAGKHIFAEKPVAVDPVGVRSII
                     ATSDLAKQKGLAIVAGTQRRHQRRYLELMKRIHDGQIGDIVAAQCYWNQGDLWVKERT
                     PNMTEMEWQCRNWLYFTWTSGDH"
BASE COUNT          431 a          949 c          947 g          358 t
ORIGIN      
        1 agacggcggc gacgcccgtc gcggcgaagg cgccggcggc ggtcagacag ccggcgcggg
       61 cctcggagac ccggcctcag cgggcggggc gcgccggcag gcgcaccgcg aggaagtagt
      121 cgcggatggg cgcggcgccc agcgcccgct gcgcgcggac cgtcaccagc cgcaggcccg
      181 attgccgcag catgcgccgg accgcggcgc gatcgtacgc gcgctcgcgc acggcgactt
      241 cgagcttccg atagtgccgg ccgcgtttca cgaaccagag ctggcgcagc agggcgacgc
      301 cggtccgggg atcgaacgcg ttgtaggccg tgaaggcgtg cggcccgagc ctgaagagct
      361 tctgactggc cgagagccgg cgcagccagt gggcggtgtt caggtcgaac tggaaaaggc
      421 cgccctcgcg aaggatgcgg ccgacgccgt cgaagacgcg ctgcagctcg gcctcgcgaa
      481 ggacgtggtt cagcgcgtcg aagtggcacg tgaccaggtc cgcgcgttcg gtgagacgca
      541 gcgccctgag atcctgccgc acccaccgga cctcgcgcga cttgcggcgc gccgcgcgca
      601 gcatggccgc ggactggtcg acgccgacca cggtccaccc gcggcgccgg gtccagggga
      661 tcgtcgagtt ccccgtgccg caggctaggt cgacgagcac gcgcggccgg agcccccgct
      721 tccggatcgc cgcgagcagc agctccctcc attcgcgata cagcgcctgt atccccgggt
      781 cggacgccat caggtcgtag tagtccgcca ggtctcggta cgggtcgtgc gccaccggat
      841 gtccctcccg ggtttcggga atgatagcgc ctggccgaag cgggccgcgc tgagatcgat
      901 cgccggccgg ttgcctgccc ttctctggta gactgctgcc cgcagcgagg cgctcccgcc
      961 ggcggcgcct cgcccggcgt ccatccgccc gttcgatccc aggaggcagc gttccatgaa
     1021 acgcagggac ttcatcgcgc ggggcctggc atccgccgcc gcgctggcgg cggcccccct
     1081 ccccgccctc gcccggcagc aagccggccc gctggcgccg cccggaccgc ccaagccgaa
     1141 gttccggctg cgctacgcgc cgagcttcgg ccagttcacc agccatgccg gcgcgggcct
     1201 cgccgcccag gtcgagttcg cctcgaacga ggggttcacg gcgatgttcg acaacggcct
     1261 catgagccgg ccgctggccg atcaggagac catcgtccgc gaactcgaga agcgcggcat
     1321 gacgctgggc cccttcgtcc tgctggccga cttcaagaac aggaacttcg tcctgcgcga
     1381 caaggacgtg cgcgccgccc tgctccagaa gatgcgcgac ggcgtcgaga cgatgaagcg
     1441 gacgggggcg aggacggccc tcgtcgtgcc cgggcgctac gacgagagcc tggcctggga
     1501 gtaccagacg gccaacctcg tcgacaatct ccgcgcgtgc atggacgtct gcgcgccggc
     1561 cgggctgacg atcgtactgg agccgctcaa cccgagggac caccccgggc tgttcctctc
     1621 gaagatcccg caggcgtacc agatctgccg ggcggtcgcc agtccgcact gcaagatcgt
     1681 gaacgacatc taccaccagc agatcagcga ggggaacatc ctgccgaaca tcgaggccgc
     1741 gtgggacgag atcgcggcct tccacgtcgg cgacaatccg ggccgcaagg agccgacgag
     1801 cggcgagatc aacttccgca acgtgttcaa gtacctccac ggccggggct accagggccc
     1861 gatctgcatg gagcacggcc agagcaagcc gggcctcgag ggggagcgcg ccgtcatcga
     1921 cgcgtaccgc tcgtgcgacg ccttctgagg agagagacga catgagccag gatcagcaca
     1981 cgacagtcac gcgccgcgat ttcgtgaaga cggcggcggc ggcctcgatg gccgcggcca
     2041 tccccggggg ggtgggcgtg ttcgcccagg ggtccgatgc gatcagggtg ggcgtcatcg
     2101 ggtgcggcgg gcgcggcacg ggggcggcca tcgactgcct caaagccgac ccggcggtgg
     2161 aaatcgtcgc cctcggcgac ctcgtgcccg accgcgtcga gtccgcgctg gctcgcctga
     2221 ccaaggaata cgcgtcgcgg gtcagggtgc cggcttcccg ccagttcgtc gggttcgaca
     2281 actacaaggg cgtctgctcg gtgccggagg tcaatctcat cgtcaccgcg gcgcctcccg
     2341 ggttccgtcc gatccatctg aaggccgcca tcgaggccgg caagcacatc ttcgccgaga
     2401 agccggtggc cgtggatccg gtgggcgtcc gctcgatcat cgccacgtcc gacctcgcga
     2461 agcagaaggg cctcgcgatc gtcgccggca cgcagcggcg gcaccagcga cgctacctgg
     2521 agctgatgaa gcgtatccac gacggccaga tcggcgacat cgtcgcggcg cagtgctact
     2581 ggaaccaggg cgacctgtgg gtgaaggagc gcacgccgaa catgacggag atggagtggc
     2641 agtgccgcaa ctggctctac ttcacctgga cctccggcga ccaca
//
LOCUS       DAOSIF010000426         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_33096_length_2680_cov_4.879619, whole genome shotgun sequence.
ACCESSION   DAOSIF010000426 DAOSIF010000000
VERSION     DAOSIF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33096_length_2680_cov_4.879619"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLT35_12060"
     CDS             complement(<1..568)
                     /locus_tag="PLT35_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30005.1"
                     /translation="MALRELKIGASGVRGVVGDALTPELIVNFACAFGTWCGGGPAVI
                     ARDTRRSSAAFRAAVVSGLLSTGCRVVDLGVGSSPLASFAVRELGASGGIVVTGSHND
                     ARWNALKFFGPDGALLNAAKNEELLDVYHGGAFARAAWDRLRPVEEARGIEDRYLEHL
                     ASALDVASIRERRFRVAVDFTNGACGAAA"
     gene            complement(570..1907)
                     /locus_tag="PLT35_12065"
     CDS             complement(570..1907)
                     /locus_tag="PLT35_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOG30006.1"
                     /translation="MSDLQQSLRPASSRRVTRVLAHGEEHPLAQASRLASRFQLARPV
                     EVHDFAEKGNINQHTYLLFAGPGEPAAYLLQRINERVFTRPRSVMRAMIASIEAQRAA
                     LAEGRLPAGDEWETITLLGTRDGRPYLDVESHRGPSCWRLMAKIPESRTFKSLGEIAE
                     PAERLRVASEAGRGLAIYGDLTCRMRTDNLENPLPGYRDTRLYYSQLRSVLAGCRTSA
                     DAGDLIPADPVLRQSAEEHFLVHLDEPEYRRRVDDAGFRPWIDLVSSEEAFALTLLEG
                     MESGRIRRLAIHGDTKLDNFLFSTRTGRVKALIDLDTIMPHTWLADWGDMVRSLVNVA
                     GEKERDLSRVDVDMDVYRAVTRGFLSAAREVTPEEIGLMADAPQIIALELGLRFLADY
                     LRGDSYFKLGPADPPDLNRVRAMAQLALFRRLREKAGEARAFIDRLRAAKEGA"
     gene            complement(2002..>2680)
                     /locus_tag="PLT35_12070"
     CDS             complement(2002..>2680)
                     /locus_tag="PLT35_12070"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30007.1"
                     /translation="GRWRAWWDFGTGSLGDMGCHILDPVFAALDLKYPISVEGCISTY
                     WEEFWKLVVPKNETYPRSAIVRYNFPARKNMPPLQLTWWDGGLMPARPAELEVGRKLG
                     DDDGGVLLVGSEATLMAGCYGRSPRLIPETRMRDFTQPAKTLERVPKGEDGHEQDWIR
                     SCKDGKPSSSNFDYSGPLSEMVLMGNLAVRFPGRLLLWDGEAMKVTNDEEANAYVRRQ
                     YREGWKL"
BASE COUNT          377 a          961 c          966 g          376 t
ORIGIN      
        1 atgcggccgc accgcacgcg ccgttcgtga agtcgacggc cacccggaac cgccgctccc
       61 ggattgacgc gacatccagg gcgctcgcca ggtgctcgag gtagcggtct tcgatgcccc
      121 gcgcctcctc gaccggacgc aggcggtccc aggccgcgcg cgcgaaggcc ccgccgtggt
      181 agacgtcgag gagctcctcg ttcttggccg cgttgaggag cgccccgtcc ggcccgaaga
      241 acttcagcgc gttccagcgc gcgtcgttgt ggctgcccgt gacgacgatg cccccggacg
      301 cgccgagctc gcggacggcg aaggacgcca gcgggctcga gccgacgccg aggtcgacga
      361 cgcggcagcc cgtcgagagc agcccggaga cgaccgccgc ccggaaggcc gcgctcgacc
      421 ggcgcgtgtc gcgcgcgatg accgccgggc cgccgccgca ccacgtcccg aacgcgcagg
      481 cgaagttcac gatcagctcg ggcgtcagcg cgtcgcccac cacgccgcgc accccggatg
      541 cgccgatctt gagctcgcgc agcgccacgt caggcccctt ccttcgccgc ccgcaggcgg
      601 tcgatgaacg ccctggcctc gccggccttc tcgcggagcc tccggaagag cgcgagctgg
      661 gccatggcgc gcacgcggtt caagtcgggc ggatccgccg ggccgagctt gaagtagctg
      721 tcgccgcgca gatagtcggc gaggaaccgg aggcccagct cgagggcgat gatctgcggc
      781 gcgtcggcca tcaggccgat ctcttcgggc gtcacctcgc gggcggccga gaggaagccg
      841 cgcgtgacgg cgcggtagac gtccatgtcc acgtccaccc gggacaggtc gcgctccttc
      901 tcgcccgcca cgttcacgag cgaccgcacc atgtcgcccc agtcggccag ccacgtgtgc
      961 ggcatgatcg tgtcgaggtc gatgagggcc ttgacgcgcc ccgtgcgcgt cgagaagagg
     1021 aagttgtcga gcttggtgtc gccgtggatc gccaggcggc ggatccggcc ggactccatg
     1081 ccctcgagca gcgtcagggc gaacgcctcc tcgctgctga ccaggtctat ccaggggcgg
     1141 aagccggcgt cgtccacgcg acgccggtac tcgggctcgt cgaggtgcac gagaaagtgc
     1201 tcctcggcgc tctgccggag cacggggtcg gcgggaatca ggtcgccggc gtcggccgac
     1261 gtccggcagc cggcgaggac cgagcggagc tgactgtagt acaggcgcgt gtcccggtag
     1321 ccgggcagcg ggttttccag gttgtcggtg cgcatccggc acgtgagatc gccgtagatg
     1381 gcgaggccgc ggccggcctc ggacgcgacg cgcaggcgct cggccggctc ggcgatctcg
     1441 ccgaggctct tgaacgtgcg cgactcgggg atcttggcca tcaggcgcca gcagctgggg
     1501 ccccgatggc tctcgacgtc caggtacggc cggccgtcgc gcgtgccgag gagcgtgatc
     1561 gtctcccatt cgtcgccggc cgggaggcgg ccctctgcca gcgcggcccg ctgcgcctcg
     1621 atcgaggcga tcatggcgcg catgaccgac cgggggcgcg tgaaaacgcg ctcgttgatc
     1681 cgctggagca ggtacgccgc cggctcgccc gggccagcga agaggagata ggtgtgctgg
     1741 ttgatgttgc ccttctcggc gaagtcgtgc acctcgaccg ggcgggcgag ctggaagcgc
     1801 gacgcgaggc ggctggcctg ggcgagcggg tgctcctcgc cgtgcgcgag cacccgcgtc
     1861 acgcgcctcg acgaagccgg ccggagggac tgctggagat cgctcatggt gcggcccggg
     1921 cggcgagggc gcgccgggcg ggccgccgcc gcgcgaacgg gcccgccgca ggagcgcgcc
     1981 gccgcaatca gcgccccgcg ctcacagctt ccatccttcg cggtactggc ggcgcacgta
     2041 ggcgttggcc tcctcgtcgt tggtcacctt catcgcctcg ccgtcccaca gcagcaggcg
     2101 ccccgggaac cgcaccgcga ggttgcccat gaggaccatc tcggagagcg ggccggagta
     2161 gtcgaagttc gacgaagagg gcttgccgtc cttgcaggag cggatccaat cctgctcgtg
     2221 gccgtcctcg cccttcggca cgcgctccag cgtcttcgcc ggctgcgtga agtccctcat
     2281 acgcgtctcg ggaatcaggc gcggactgcg gccgtagcag ccggccatga gcgtcgcctc
     2341 gctcccgacg agcagcacgc cgccgtcgtc atcgcccagc ttgcgcccca cttccagctc
     2401 ggccggccgg gcgggcatca gcccgccgtc ccaccacgtt agctggagcg gcggcatgtt
     2461 cttccgagcc gggaagttgt agcgcacgat ggccgagcgc gggtacgtct cgttcttcgg
     2521 cacgacgagc ttccagaact cctcccagta ggtcgagatg cagccctcga cgctgatcgg
     2581 gtacttgagg tccagcgccg cgaagaccgg gtcgaggatg tggcagccca tgtcgccgag
     2641 cgagccggtg ccgaagtccc accacgcgcg ccagcggccg
//
LOCUS       DAOSIF010000427         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_33314_length_2666_cov_3.196093, whole genome shotgun sequence.
ACCESSION   DAOSIF010000427 DAOSIF010000000
VERSION     DAOSIF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33314_length_2666_cov_3.196093"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLT35_12075"
     CDS             <1..313
                     /locus_tag="PLT35_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30008.1"
                     /translation="NATDKDVDLNFPVEIVSTAVKVEQASGGIITPPTGGETEHYDSV
                     LSGATGNKISAVGGSVSMNFEVWYDLPSLKKEAAITITLNLKDDGGKTFAKMIKVNVA
                     P"
     gene            complement(361..1785)
                     /gene="gatD"
                     /locus_tag="PLT35_12080"
     CDS             complement(361..1785)
                     /gene="gatD"
                     /locus_tag="PLT35_12080"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOG30009.1"
                     /translation="MNPDTTERPDRLQGYRGPAMAVLDRFGVGVWSEVEVTNDDGSIF
                     AGVILPRNETCDDLHIVIKLFNGYNVGVAASRVVRAVETGYRRAVYKIPEKDFPFDPR
                     KKNVTLLGTGGTIASRLDYRTGAVIPAFTPGELYGAVPELADIANLKTKRLFGVFSEN
                     MGPEQYKALAVAIGEEIEQGADGIVIGHGTDTMAHTGAILSFMVQQSPVPIVLVGSQR
                     SSDRPSSDAALNLMNAVRTAAECDIAEVMLCMFGPTSDNYDLLHRGTRCRKMHSSYRS
                     TFRTVGATPLATVSRYAREDGGHFTYLTQDVLRRDPSRRPVITPVFEEKVTIQYYYPN
                     FNPDIIDSLSGLGYRGLVIAGTGLGHVNKPLYPAIRRAVARGMTVVMTVQTLWGFAQM
                     YVYDTGRDLLDLGIIPLDNMLPETAYMKLAWVLGQTEDPARIREMMQTAVNHEITPRE
                     PHNGYLILQGGLPEVEAFVQRHWK"
     gene            complement(1782..>2666)
                     /locus_tag="PLT35_12085"
     CDS             complement(1782..>2666)
                     /locus_tag="PLT35_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30010.1"
                     /translation="VIACLDEVPIVFSGADFPQFQGRNQVLDRVRRRADVGASDEVFI
                     VFGPEDDVRTAAEEIRLRFADATAGVPKETRQALPGGYTTFERILPGPDRMYPDTDSP
                     PTRVTAERVAAIRAGLKPPPWTRLERYAGWRVPAETSEFLIRRGGADIVDAVVESTGV
                     DGLTAAVEIGQRAKALARRGVPVGRLGAAEWAAVFDLYASGRIAREAIAAVASRVAAD
                     GLTAEAAASAEGAWLAPRELWLRELRELVAGAGAATGVGDAAARRLRWLTGRAVRALK
                     GRAPAREVAAWLAAEAGR"
BASE COUNT          397 a          965 c          905 g          399 t
ORIGIN      
        1 gaacgccacg gacaaggacg tggatctgaa cttcccggtc gagatcgtct cgacggcggt
       61 caaagtcgag caggcgtcgg gcggcatcat cacgccgccg acgggcggcg agaccgagca
      121 ctacgactcg gtgctgagcg gcgcgaccgg gaacaagatc tccgcggtgg gcggcagcgt
      181 ctcgatgaac ttcgaggtgt ggtacgacct gccgagcctc aagaaagagg ccgcgatcac
      241 catcacgctc aacctcaagg acgacggcgg caagaccttc gcgaagatga tcaaggtcaa
      301 cgtcgccccg taggcgacgg gcccgcgcgc gccgcgaggg ccggccgtca agccggccct
      361 tcacttccag tgccgctgga cgaacgcctc gacctcgggc aggcccccct gcaggatcaa
      421 gtagccgttg tggggctccc gaggcgtgat ctcgtggttg acggccgtct gcatcatctc
      481 ccggatccgc gccggatcct ctgtctggcc gagcacccac gccagcttca tgtaggccgt
      541 ctcgggcagc atgttgtcca gggggatgat ccccaggtcg agcaggtcgc ggccggtgtc
      601 gtagacgtac atctgcgcga atccccagag cgtctggacc gtcatcacga ccgtcatgcc
      661 gcgcgccacc gcccgccgga tggccgggta gaggggcttg ttcacgtggc cgaggcccgt
      721 cccggcaatg accagcccgc ggtacccgag cccggacagg ctgtcgatga tgtccgggtt
      781 gaagttcggg tagtagtact ggatcgtcac cttctcctcg aacaccggcg tgatgaccgg
      841 gcgccgcgac gggtcgcgcc tcaggacgtc ctgcgtcagg tacgtgaagt ggccgccgtc
      901 ctccctggcg tagcggctca ccgtcgcgag cggcgtggcg cccaccgttc ggaacgtcga
      961 gcggtagctg gagtgcatct tgcggcagcg cgtcccgcgg tgcagcaggt cgtagttgtc
     1021 cgaggtcggg ccgaacatgc agagcatcac ctccgcgatg tcgcactccg ccgcggtgcg
     1081 gaccgcgttc atcaggttca gcgccgcgtc agacgagggg cggtccgatg accgctggct
     1141 gcctacgagg acgatgggca cgggcgactg ctggaccatg aagctcagga tcgcgcccgt
     1201 gtgcgccatc gtgtcggtcc cgtggccgat cacgatgccg tcggcgccct gctcgatttc
     1261 ctcgccaatc gcgacggcga gggccttgta ctgctccggc cccatgttct cgctgaacac
     1321 gccgaacagc cgcttcgtct tcaggttcgc gatgtccgcc agctccggca ccgccccgta
     1381 cagctcgccc ggcgtgaacg ccggaatgac cgcgcccgtc cggtagtcga ggcggctcgc
     1441 gatggtgccg ccggtgccga ggagcgtgac gttcttcttg cgcggatcga acgggaagtc
     1501 cttctccggg atcttgtaga cggcccgccg gtaacccgtt tcgacggccc gcaccacgcg
     1561 gctcgcggcc acgccgacgt tgtagccgtt gaacagcttg atcacgatgt gcaagtcgtc
     1621 gcaggtctcg ttgcgaggga ggatgacgcc ggcgaagatc gacccgtcgt cgttcgtgac
     1681 ttcgacctcg ctccagacgc cgaccccgaa ccggtcgagg accgccatgg ccgggccgcg
     1741 atacccctgg aggcgatcgg gccgctcggt cgtgtccgga ttcatcgccc cgcctccgcg
     1801 gccagccagg ccgcgacctc gcgtgccggg gcccggccct tcagcgcgcg cacggcgcgc
     1861 cccgtgagcc agcgcagccg tctggccgcc gcgtcaccga cgccggtcgc cgcaccagcg
     1921 cccgcgacca gctccctgag ttctcgcagc cacagttccc gcggcgcgag ccaggcgccc
     1981 tcggccgacg cggcggcctc ggcagtgagc ccgtcggcgg ccacgcgcga cgccacggcg
     2041 gcgatggcct ccctggcgat ccggcccgag gcgtagagat cgaacaccgc ggcccactcg
     2101 gccgcgccca gccgtcccac cggaacgccc ctccgcgcga gggccttcgc gcgctggccg
     2161 atctcgacgg ccgccgtcag gccgtcgacg cccgtcgact cgacgacagc gtccacgatg
     2221 tcggcgcccc cgcgacggat gaggaactcc gacgtctcgg ccgggacccg ccagccggcg
     2281 tagcgctcga ggcgggtcca gggcggcggc ttcaagccgg ccctgatcgc cgcgacccgc
     2341 tcggccgtca cgcgtgtcgg cggcgaatcc gtgtccgggt acatgcggtc cggtccaggc
     2401 aggatccgct cgaaggtcgt gtagccgccg ggcagcgcct gccgcgtctc tttcggcacg
     2461 ccggccgtgg cgtcagcgaa gcgcagccgg atttcctccg cggccgtccg cacatcgtcc
     2521 tccgggccga agacgatgaa cacttcgtcg gacgcgccca cgtccgcgcg ccgcctgacc
     2581 cgatcgagca cctggttgcg cccctggaac tgggggaagt ccgccccgga gaacacgatg
     2641 ggcacctcgt cgaggcaggc gatgac
//
LOCUS       DAOSIF010000428         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_33383_length_2661_cov_3.475058, whole genome shotgun sequence.
ACCESSION   DAOSIF010000428 DAOSIF010000000
VERSION     DAOSIF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33383_length_2661_cov_3.475058"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLT35_12090"
     CDS             <1..324
                     /locus_tag="PLT35_12090"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30011.1"
                     /translation="RGEALVVDALAHAAELAAAARVALLDGDAERFSRLLDENFDTRR
                     RIYALPPWQIAQVEAARACGASAQFAGSGGAIVGVYRDAGMLARVRAALEAVGSRTIV
                     PQIAA"
     gene            complement(331..1767)
                     /gene="uxaC"
                     /locus_tag="PLT35_12095"
     CDS             complement(331..1767)
                     /gene="uxaC"
                     /locus_tag="PLT35_12095"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOG30012.1"
                     /translation="MKPFLGEDFLLESDVASDLYRRFAAGMPIIDYHCHLSPALMAAD
                     HRFATITELWLDGDHYKWRAMRSNGVPERFCTGDAGPWEKFEAWARTVPDTLRNPLYH
                     WTHMELRRPFGVTDLLSPATARRVFDRCNERLQEDGFTALGLLRQFRVAAVCTTDDPV
                     DSLEHHRTLQARPDPDTRVYPTWRPDQLLAVEDPQAFNAYVGRLEQASGLPVGGRLRS
                     LLDALEARHDVFHEMGCRASDHGLETMYAERWTDGEADEALGRLRGGRALEPGPALKL
                     KSALLHALAVLDHARGWVQQYHLGALRNNNTRLRRALGPDTGFDSIGDFEIARPLSRF
                     LDRLDEADRLARTILYNLNPRDNALMATMIGNFQDGTVPGKLQYGSGWWFLDQLDGMT
                     AQMDALSNMGLLSRFVGMVTDSRSFLSYSRHDYFRRLLCNLLGEDVRRGRLPDDRDLL
                     GRLVSNVCFFNARDYFGFALGRAAEGAS"
     gene            complement(1769..>2661)
                     /locus_tag="PLT35_12100"
     CDS             complement(1769..>2661)
                     /locus_tag="PLT35_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG30013.1"
                     /translation="RRERAFATAIFNSGTNVGAIIAPAMVPAIALTLGWRAAFIVAGL
                     LGFLWLLLWLPFYDVPAKKLKASSPELAHITSDRDEAGDGATPLGWGRALRIRQTWGF
                     IVAKFMTDPVWWFFLIWLPDYFKKTRGLDIKHSWIHLVTIYAIITVLSILGGWLTGHF
                     QQQGWSVTRARKTGMFLFALCVVPIAVVTKVGPWSAVLLIGLAGAAHQAWSANLFTTT
                     SDVFQKKDVGTITGLGGMAGSIGGILFPWVTGAMLDSFTAAGNVTGGYAILFGICASL
                     YLVAFAIHHLLVPRLEPLER"
BASE COUNT          407 a          938 c          945 g          371 t
ORIGIN      
        1 cgcggggagg cgctggtggt cgacgccctg gcgcacgccg cggagctggc ggccgccgcg
       61 cgcgtcgccc tgctcgatgg cgacgcggag cggttctcgc ggctgctgga cgagaacttc
      121 gacacgcggc ggcggatcta cgccctgccg ccctggcaga tcgcccaggt cgaggcggcg
      181 cgcgcctgcg gggccagcgc gcagttcgcc ggctccggcg gcgcgatcgt cggcgtctac
      241 cgggacgcgg gcatgctggc gcgggtgcgc gcggccctcg aggccgtcgg ctcgcgcacg
      301 atcgtgcccc agatcgccgc ctgagcccgc ctacgacgct ccctccgcgg cccggccgag
      361 cgcgaacccg aagtagtccc gcgcgttgaa gaagcagacg ttgctcacca gccgcccgag
      421 cagatcgcga tcgtccggca gacggcctcg ccgcacgtcc tccccgagca ggttgcagag
      481 cagccgcctg aagtagtcgt ggcgcgagta cgagaggaag cttcgcgagt cggtcaccat
      541 cccgacgaac cgcgagagca ggcccatgtt cgacagggcg tccatctgcg ccgtcatgcc
      601 gtcgagctgg tcgaggaacc accagccgga gccgtactgc agcttgcccg gcaccgttcc
      661 gtcctggaag ttcccgatca tcgtggccat cagcgcgttg tcgcgcgggt tcaggttgta
      721 caggatcgtc ctggccaggc gatccgcctc gtcgaggcgg tccaggaagc gcgagagcgg
      781 ccgcgcgatc tcgaagtccc cgattgagtc gaagcccgtg tccggcccca gcgcgcgccg
      841 cagccgggtg ttgttgttgc gcagcgcgcc gaggtggtac tgctgcaccc agccgcgggc
      901 gtggtccagg acggccagag cgtggagcag cgcggacttc agcttcagcg ccgggccggg
      961 ctcgagcgcg cgcccgcccc tcagccgccc gagcgcctcg tcggcctcgc cgtcggtcca
     1021 cctctcggca tacatcgtct ccaggccgtg atccgaggcg cggcagccca tctcgtggaa
     1081 gacgtcgtgg cgggcctcga gcgcgtccag cagcgaccgc agccgccccc cgaccggcag
     1141 gcccgacgcc tgctcgagcc ggcccacata cgcgttgaac gcctgcgggt cctcgacggc
     1201 cagcagctgg tcggggcgcc aggtcggata cacgcgcgtg tcgggatcgg gcctcgcctg
     1261 gagcgtccgg tggtgctcca gcgaatcgac cggatcgtcg gtcgtgcaga ccgccgcgac
     1321 gcggaactgc cgcagcaggc cgagcgccgt gaagccgtcc tcctgcaggc gctcgttgca
     1381 gcgatcgaac acgcggcggg ccgtcgccgg cgacagcagg tcggtcaccc cgaacgggcg
     1441 cctcagctcc atgtgcgtcc agtggtagag cggattgcgc agcgtgtccg gcaccgtgcg
     1501 cgcccacgcc tcgaacttct cccacggccc ggcgtcaccc gtgcagaacc gctccggcac
     1561 gccgttcgac cgcatggccc gccacttgta gtggtcgccg tcgagccaga gctcggtgat
     1621 ggtcgcgaag cggtggtccg ccgccatcag cgccggcgac aggtggcagt ggtagtcgat
     1681 gatcggcatg ccggcggcga accggcggta gaggtccgac gcgacgtcgc tctcgagcag
     1741 gaagtcctcg ccgaggaagg gtttcatgtc atcgctccag cggctcgagc ctcgggacga
     1801 gcaggtgatg gatggcaaag gcgacgagat agagcgacgc gcagatgccg aagaggatgg
     1861 cgtagccgcc ggtcacgttg cccgcggcgg tgaagctgtc gagcatcgcg ccggtcaccc
     1921 aggggaacag gatgccgccg attgaccccg ccatcccgcc caggccggtg atcgtcccca
     1981 cgtccttctt ctggaagacg tcggaggtcg tcgtgaagag gttggcggac caggcctggt
     2041 gcgcggcccc cgccaggccg atgagcagca cggcggacca ggggccgacc ttcgtcacca
     2101 ccgcgatcgg gacgacgcac agcgcgaaca ggaacatgcc ggtcttgcgc gcccgcgtca
     2161 cgctccagcc ctgctgctgg aaatgcccgg tgagccaccc gcccaggatg ctcagcaccg
     2221 tgatgatcgc gtagatcgtc acaaggtgga tccagctgtg cttgatgtcg aggccgcggg
     2281 tcttcttgaa gtagtcgggc agccagatca ggaagaacca ccagaccgga tcggtcatga
     2341 acttcgcgac gatgaagccc caggtctggc ggatgcggag ggcgcgcccc cagccgaggg
     2401 gcgtggcgcc gtcgccggcc tcgtccctgt cgctggtgat gtgggccagc tccggcgacg
     2461 aggccttcag cttcttcgcc ggcacgtcgt agaacgggag ccacaggagc agccacagga
     2521 accccaggag gcccgccacg atgaaggccg cgcgccagcc gagcgtcagc gcgatcgccg
     2581 gcaccatcgc cggggcgatg atcgccccga cgttcgtgcc cgagttgaag atggccgtcg
     2641 cgaaggcgcg ctcgcgcctc g
//
LOCUS       DAOSIF010000429         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_33385_length_2661_cov_3.000767, whole genome shotgun sequence.
ACCESSION   DAOSIF010000429 DAOSIF010000000
VERSION     DAOSIF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33385_length_2661_cov_3.000767"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2655
                     /locus_tag="PLT35_12105"
     CDS             <1..2655
                     /locus_tag="PLT35_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG30014.1"
                     /translation="MHANHPEVTEPWLRTVFTHEFAHILHLDRVGGAMKALRRALGRH
                     PATFPNLFLPEWQIEGFATWAEGAASGFGRPRAADVAAVIEASSGARGLPIDRAGGGL
                     VAWPSGHTPYFFGGRFYQDLAGRTSEAALGDFTRQTARRLPYFGGGAFRRVFGESERN
                     AWRRALADAHAESGRATLEPAALRRLTRDGFAVGGPRVIRAGKESTGEEPAVYYSTQG
                     PHRFPEIRQVRLAGGRSAPVTPRYGGDTLSSDGRWVFFDQLEYDASVALVSDLYALEL
                     ATGRVCRLSRGRRLTDPDVDGRAARLVAIEAASGRRRPVILALERDTAGRPALAAVPA
                     TTMGTAGCDYASPRWSPDSTRIAAARQCPGALPAIVVFGADGSGEIAIVQPAGARDIT
                     PAWLADGRTLIFASDRQDRRFKLFAVSDPGQAQESGHREPVLLVDAPGGVLWPDPAPD
                     GRTVVFTSMTADGYDVFASALPESPPRPVQTAGPVAGREGPAGPVRTPAAAAGVRAGT
                     EDREEPATAVPYSPWRTLVPRAWWPIGAVSGDRLDLGGSVGASDALGYHEYRLSASWR
                     ASSERVDLAFEGAALEWSAWYAYNRWTPSFFVSAKDSIETVRLATPAPGGPLTSDERT
                     REVFAGVLVPWRCARTAQRWLAGVDLETRRLPGSAGIADRSRRAARAAWAFSSAHAFG
                     YSISPERGVRAGATLERVSPALGADGQATSVTADARAYVPAFGRHDVLAVRAAVGSSR
                     GDRPLRRQYRLGETALAGPGSSFGSDAVGLLRGLAKRPAAGHAIAVANLDYRFPVARV
                     ERGVRTWPVFLRDLHGAVFVDAGATGADLGSLGRPAWSAGAEFSSRLTLGYSWLFNLT
                     AGVAWVHDPARADPKGRAAAFVRTGYAF"
BASE COUNT          315 a          980 c          978 g          388 t
ORIGIN      
        1 atgcacgcga accatcccga ggtgacggag ccgtggctgc ggaccgtctt cacgcacgaa
       61 ttcgcccaca tcctgcacct cgaccgcgtg ggcggggcca tgaaggccct tcgccgggcg
      121 ctcggccggc atcctgccac gttcccgaac ctgttcctgc ccgagtggca gatcgagggg
      181 ttcgcgacgt gggcggaggg cgccgccagc ggcttcggcc gcccgcgcgc tgccgacgtc
      241 gccgccgtca tcgaggcctc atccggcgcg cgcggcctgc cgatcgaccg ggctggaggc
      301 ggcctcgtcg cctggccatc cggccacacg ccgtacttct tcggcggccg gttctaccag
      361 gatctggccg gccgcacctc ggaggccgca cttggggact tcacgcgcca aaccgcgcgc
      421 cggctcccct acttcggagg cggagcgttc cgtcgggtct tcggcgaatc ggagcggaac
      481 gcctggcggc gggcgctggc ggacgcgcac gcggaaagcg ggcgcgcgac gctcgaaccg
      541 gcggcgctgc ggcgcctgac gcgcgatggc ttcgccgtgg gcgggccccg agtgattcgt
      601 gccggcaagg agagcacggg cgaggagcct gccgtctact acagcaccca gggaccgcat
      661 cgctttccgg agatccgcca ggtccgtctc gcaggcggcc ggtcggcgcc ggtgaccccg
      721 cgctatgggg gcgacacgct gtcgagcgac gggcgctggg tgttcttcga ccagctcgag
      781 tacgacgcct ctgtggcgct cgtgtcggac ctctacgccc ttgagctcgc aaccggacgg
      841 gtctgccgct tgtcgcgggg acgtcgcctg accgaccctg atgtcgacgg ccgcgcggct
      901 cgcctggtcg ccatcgaggc cgcgagcggc cggcgtcggc ccgtcatcct cgcgctcgag
      961 cgcgacaccg ccggccggcc cgcgctggcg gccgttccgg cgaccacgat gggcacggcc
     1021 ggatgcgatt acgcatcgcc gcgctggtct cccgattcca cgcgcattgc cgccgcgcgg
     1081 cagtgccccg gagcgctgcc ggccatcgtg gtgttcggcg cagacggatc aggggagatc
     1141 gcgattgtgc aaccggccgg cgccagggac atcacgccgg catggcttgc agacggacga
     1201 acgctgatct tcgcctccga ccggcaggat cgtcgcttca agctgttcgc tgtgagcgac
     1261 cccgggcaag ctcaggagag cggacaccgt gagccggtcc tgctcgtcga tgcgcccggc
     1321 ggcgtgttgt ggccggatcc cgcgccggat ggacggaccg tcgtgttcac gagcatgacg
     1381 gcggacggct acgacgtctt cgcatcggcg ctgcccgagt ccccgccgcg cccggtgcaa
     1441 acggccgggc ccgttgcggg gcgcgagggg ccggccgggc cggttcggac cccggctgcg
     1501 gccgccggcg tgcgagccgg gacggaggac cgtgaggagc cggcgaccgc ggtcccctac
     1561 tcgccgtggc gcaccctcgt cccccgcgcc tggtggccca tcggcgccgt ctccggcgat
     1621 cgcctggatc tcggcgggag cgtcggcgcg tcggatgcgc tcggctacca cgagtaccgg
     1681 ctgtcggcgt cgtggcgcgc ttcgagcgag cgcgtggacc tggcgttcga gggcgccgcc
     1741 ctcgaatgga gcgcctggta cgcttacaac cgctggacgc cgtcgttctt cgtgtccgcg
     1801 aaggactcca tcgaaaccgt gcgcctcgcc acgcctgcgc ccgggggccc gctcacgtcc
     1861 gacgagcgca cgcgcgaggt cttcgccggc gtcctcgttc catggcgctg tgcgagaacc
     1921 gcacagcgct ggctggctgg cgtggatctc gaaacgcggc gcctccccgg ctctgcgggc
     1981 atcgcggacc ggagccgccg cgccgcgcgt gcggcgtggg ccttctccag cgcgcacgcc
     2041 ttcggctact ccatcagccc cgagcggggc gtcagggccg gcgcgacgct cgagcgcgtg
     2101 agtcctgcgc ttggcgctga cgggcaggcc acctccgtga ccgccgacgc ccgggcgtac
     2161 gtccctgcct tcggccgcca cgacgtgctg gccgtgaggg ccgctgtggg atcgagccgc
     2221 ggcgaccggc cgcttcggcg ccagtaccgc ctgggcgaaa cggcactggc gggacccggc
     2281 tccagtttcg gcagcgacgc cgtcggcctc ctgcgcggcc tggcgaagag gccggccgcc
     2341 ggccacgcga ttgccgtggc gaacctcgat taccgcttcc ccgtggcgcg cgtcgagcgc
     2401 ggggtgcgca cgtggccggt gttcctgcgt gatctccacg gcgcggtgtt cgtcgacgcg
     2461 ggtgcgacgg gagcggacct gggaagcctg ggccggcccg cctggtccgc cggcgcggag
     2521 ttctcgtcgc gcctcacgct cggctattcc tggctcttca acctcacggc gggcgtcgcc
     2581 tgggtacacg accccgcccg ggcggacccg aaaggaaggg cagccgcttt cgtccggacg
     2641 ggatacgcgt tctgacggcg c
//
LOCUS       DAOSIF010000430         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_33527_length_2653_cov_3.127791, whole genome shotgun sequence.
ACCESSION   DAOSIF010000430 DAOSIF010000000
VERSION     DAOSIF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33527_length_2653_cov_3.127791"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..741
                     /locus_tag="PLT35_12110"
     CDS             16..741
                     /locus_tag="PLT35_12110"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOG30015.1"
                     /translation="MMRFSDACGAEIPRWIRLRLQSFGDDTASIKAFGLDVVTDLCEQ
                     LRAGGAPAIHFYTMNQSGPTLPERGVALKAELRRFFDVPGVRQAGSLVAADTDHLWSA
                     NGTASVFRPAGRRGKLFARASGGWSFGETALVNGFSLGGPFELGAYFPNELRGSNCAV
                     ANLGYFQEIGRFVEGAIGRLHAGAWIERGAAFERLRSADFRTNVSAGLLLESPIGPVF
                     AGVSLGEHGRYRVFFSLGRFLPH"
     gene            complement(825..1928)
                     /locus_tag="PLT35_12115"
     CDS             complement(825..1928)
                     /locus_tag="PLT35_12115"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HOG30016.1"
                     /translation="MDVTASPLPDGPHRRYNPLLDEWVLVSPHRLKRPWQGQVEPEPP
                     PMLPAYDPNCYLCPGNERASGERNPSYETTFAFDNDFPALVPSFGASPAASGARGPGD
                     SLFVAEPQTGRCRVVCFSPRHDVTLAQMDTPGIRAVVDAWADEIERLSRFDAVRYVQV
                     FENKGAAMGCSNPHPHCQIWASASVPHIPARKLEMQRRYAARTGRDLVGDYVAREIAS
                     GERVVCRNDAWAVVVPFWAVWPFETMIAPLRFVGDLRELSDDERGQLADIIRSLTARY
                     DNLFQSSFPYSMGWHGRPVDGGDYPGCRLHAVYFPPLLRSASVRKFLVGYEMTGEPQR
                     DLTAESAAERLRGLPEVHYLDGAAARPGASPGS"
     gene            1964..>2653
                     /locus_tag="PLT35_12120"
     CDS             1964..>2653
                     /locus_tag="PLT35_12120"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG30017.1"
                     /translation="MSVIPGLTSLLASRRLDGLRVGLVCNPASVDANLDHAADLLLRA
                     PGVTLGALFGPQHGFRADLQDNMIESPHATDARRRVPVYALYSETREPTAAMLRGLDV
                     LVIDLQDVGARIYTFVYTMANCLRAAARHGLPAIVCDRPNPVGGDALEGPMLEPGYES
                     FVGQFPIPMRHGMTIGELAALFNDHFGIGADLTVVRMEGWARSQYFDQTGLPWVMPSP
                     NMPTLETAIVYP"
BASE COUNT          383 a          935 c          943 g          392 t
ORIGIN      
        1 accagttcca cgcagctgat gcgtttttca gacgcctgtg gcgccgagat tccacgctgg
       61 attcggttgc gcttgcaaag ctttggcgac gatacggcgt ccatcaaggc gtttggcctg
      121 gatgtggtga cagacctttg cgagcagttg cgcgcgggtg gggcacctgc gattcacttc
      181 tacacgatga accagagcgg gccgacgctg cccgaacgcg gggtcgcgct caaggcggaa
      241 ctgcggcggt tcttcgacgt gcccggggtc cgccaggccg gcagcctggt ggccgccgac
      301 acggatcacc tctggtcggc gaacgggacg gcgtcggtct tcagaccggc gggccggcgc
      361 ggcaagctct tcgcgcgcgc gagcggcggc tggtcgttcg gcgagacggc cctcgtcaac
      421 ggattcagcc tgggcggccc gttcgagctc ggcgcgtact tcccgaacga actccggggc
      481 agcaattgcg ccgtcgcgaa cctgggctac ttccaggaga tcggccgctt tgtcgaaggc
      541 gcgatcggcc gactccacgc gggcgcgtgg atcgagcgcg gagccgcgtt cgagcggctc
      601 aggagcgcgg atttcaggac gaacgtctcg gcgggcctgc tgctcgaatc acccatcggg
      661 ccggtgttcg ccggcgtcag cctcggcgag cacgggcggt accgggtctt cttcagcctc
      721 gggcgcttcc tgccgcacta gccagcgacg aaggaagcgt cagccgagca cgcgggacgc
      781 ggggccgccg cgcgcgaagc cggctgcaca cgagccgcgc cgggctacga gccgggcgac
      841 gcgccgggcc gcgcggccgc gccgtccagg tagtgcacct ccgggaggcc gcgcagccgc
      901 tccgcggcgg actcggcggt gaggtcgcgc tggggttcgc cggtcatctc gtagccgacc
      961 aggaacttcc gcaccgacgc cgaccgcagc agcggcggga agtagaccgc gtgcagccgg
     1021 caccctggat agtcgccgcc gtccaccggg cgcccgtgcc accccatcga gtacgggaac
     1081 gacgactgga acaggttgtc gtagcgtgcg gtcaggctgc ggatgatgtc cgccagttgc
     1141 ccgcgctcgt cgtccgagag ctccctgagg tcgcccacga agcgcagcgg cgcgatcatc
     1201 gtctcgaacg gccagacggc ccagaacggc accaccaccg cccacgcgtc gttgcggcag
     1261 accactcgtt cgcccgaggc gatctcgcgc gccacgtagt cgccgacgag atcgcgcccc
     1321 gtgcgcgccg cgtaccggcg ctgcatctcg agcttgcggg ccgggatgtg cggcaccgag
     1381 gcggacgccc agatctggca gtgcggatgg gggttgctgc accccatcgc cgcgcccttg
     1441 ttctcgaaga cctgcacgta gcggacggcg tcgaaccggc tcagccgctc gatctcatcg
     1501 gcccacgcgt ccaccacggc ccggatgccc ggcgtgtcca tctgcgcgag cgtgacgtcg
     1561 tggcgcggcg agaagcacac gacgcggcac cggccggtct gcggctccgc cacgaacagg
     1621 ctgtcgccgg gcccgcgtgc gccgctggcc gcaggcgacg cgccgaaaga cggcaccagc
     1681 gccgggaagt cgttgtcgaa ggcgaacgtc gtctcatagg acgggtttcg ctcgccgctt
     1741 gcccgctcgt tgccggggca caggtagcag ttcgggtcgt aggcgggcag catcggcggc
     1801 ggctccggtt cgacctggcc ctgccagggg cgcttgagcc ggtgagggga cacgagcacc
     1861 cactcgtcca ggagcgggtt gtagcggcgg tgcggtccgt cgggaagcgg cgaggctgtc
     1921 acgtccacgc gggcagcata caccgggatg gtatagtcgg cccatgagcg tcattcccgg
     1981 tctgaccagc ctgcttgcgt cgcgccggct cgacggcctc cgcgtcggcc tcgtctgcaa
     2041 ccccgcgtcg gtagacgcga acctggacca cgccgcggac ctgctcctgc gcgcgccggg
     2101 cgtgacgctc ggggcgctct tcgggccgca gcacggcttc cgcgcggacc tgcaggacaa
     2161 catgatcgag tcgccgcacg ccaccgacgc ccgccgccgc gtgcccgtgt acgcgctcta
     2221 cagcgagacg cgcgagccga cggcggccat gctgcggggc ctcgacgtcc tggtgatcga
     2281 cttgcaggac gtcggcgcgc gcatctacac cttcgtctac acgatggcca actgcctgcg
     2341 cgccgccgcg cgccacggcc tgccggccat cgtgtgcgat cggccgaacc ctgtcggcgg
     2401 cgacgccctg gagggcccga tgctcgagcc gggctacgag tccttcgtcg ggcaattccc
     2461 catcccgatg cgccacggga tgacgatcgg cgaactggcg gcgctgttca acgatcactt
     2521 cggcatcggg gcggacctga cggtcgtccg catggagggc tgggcgcgca gtcagtattt
     2581 cgaccagacg ggactgccgt gggtgatgcc gtcgccgaac atgcccacgc tcgagaccgc
     2641 catcgtctac ccg
//
LOCUS       DAOSIF010000431         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_33585_length_2649_cov_3.423670, whole genome shotgun sequence.
ACCESSION   DAOSIF010000431 DAOSIF010000000
VERSION     DAOSIF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33585_length_2649_cov_3.423670"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLT35_12125"
     CDS             <1..351
                     /locus_tag="PLT35_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30018.1"
                     /translation="TPVVLCARTAREFQAISRDADPVPTALREFRRTDRLLVRFFAHS
                     ATAGPPDVGARLLNRGGQKMLDLPLAASPDPAQPHQVDLPLASFSPGEYVIEIRARDV
                     SGEATEFVGFRLIG"
     gene            complement(631..1914)
                     /locus_tag="PLT35_12130"
     CDS             complement(631..1914)
                     /locus_tag="PLT35_12130"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOG30019.1"
                     /translation="MTKPFNPFEMAQAQFDRVADLLGLDQATRELLRWPLREYQFAIP
                     VRMDDGTVRVFRGFRVQHNDARGPNKGGIRFHPNETLDTVRALATWMTWKCAVVDIPL
                     GGGKGGVVCDPHQLSLREQEAICRGWVRQLSKNVGYLQDVPAPDVMTNPQHMLWMMDE
                     IEVLHGQKEPGFITGKPVGSGGSLGRTEATGYGVIYTVREALRLLGLDPASMTASVQG
                     FGNVAQFAVQLFTQYGGRVVCVSCWDHADRTSYSFRRPSGITYEELIGITDRFGTVDK
                     LKAKTLGYEILPGDAWIEQAVDVLIPAAIENQVTADTARRIHQKVKLVAEGANGPTTP
                     EADAILQERGIYVIPDFLCNAGGVTCSYFEQVQGNMNFFWPKHEVLERLDAVMTRAFH
                     AVADLAKTKQLYTRDAAYVIAINRVANACRLRGWV"
     gene            complement(1980..>2649)
                     /locus_tag="PLT35_12135"
     CDS             complement(1980..>2649)
                     /locus_tag="PLT35_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30020.1"
                     /translation="AIAARQAARSVGFALPGARVAVLGFDGVCEEAAVVLERMGARVI
                     AAADARGGVYEPRGLDVGALLEHRRQSRTVDGFAGGGPLGIGDVLGLEADILLAGTAA
                     SPVTEAAAGSVRAPLVVEASVGATTAEACRVLQERGLLVVPEILSGAGRATAAHLEWE
                     QNRRGDSWSEADVHQRLDARMGAAFEAVFGAARKYETDLRTAATVVAIARVAAATRVR
                     GMYA"
BASE COUNT          365 a          978 c          914 g          392 t
ORIGIN      
        1 acacccgtcg tattgtgcgc gcgcaccgcc cgcgagttcc aggcgatctc gcgcgacgcc
       61 gatccggtgc cgaccgcgct gcgcgagttc cggcggacgg accggctcct ggtgcgcttc
      121 ttcgcgcact cggcaaccgc cgggccgccc gacgtcggcg cgcggctgct caaccgcggc
      181 ggccagaaga tgctggacct cccgctggcc gcgtcgcccg acccggcgca accccaccag
      241 gtggacctgc cgctggcgag cttctcaccg ggcgagtacg tcatcgagat ccgggccagg
      301 gacgtgtcgg gcgaggccac tgagttcgtg gggttcaggc tgatcggctg aagaggccgg
      361 ccgtcctccg ctctcgccgc gtcgaggcgc tcacgcaccg ccggttcgaa cgtcagcttc
      421 gcctgggacg cggagcgctt ccagcccgcc ggcctggcct cgcgcgcgtg tcccggcgcg
      481 cttcattcgc gggccgcccg gaatgcgcag caagatcctg cgccaggcag aagcagctca
      541 gcgccgacgc ccgggtgcgc gggcgatggc ggcgatggac gggcggtcag gcaggaggat
      601 gcggaggcgc gggacaaggg ctggcggctc ctacacccac cccctcagcc tgcaggcgtt
      661 cgccacgcgg ttgatcgcga tgacgtacgc ggcgtcgcgg gtgtagagct gtttcgtctt
      721 cgccaggtcg gccacggcgt ggaacgcccg cgtcatgacg gcgtccagcc gctcgagcac
      781 ttcgtgcttc ggccagaaga aattcatgtt cccctgcacc tgctcgaagt agctgcaggt
      841 cacgccgccg gcgttgcaca ggaagtcggg gatcacgtag atcccgcgct cctgcaggat
      901 cgcgtccgcc tccggggtcg tcggcccgtt tgcgccctcg gcgacgagct tcaccttctg
      961 gtggatccgc cgcgcggtgt cggcggtcac ctggttctcg atggccgcgg ggatgagcac
     1021 gtcgaccgcc tgctcgatcc acgcgtcgcc gggcaggatc tcgtagccga gggtcttcgc
     1081 cttgagcttg tccaccgtgc cgaatctgtc ggtgatgccg atgagctcct cgtacgtgat
     1141 gccggacggc cggcggaacg agtacgacgt ccgatccgcg tggtcccagc acgacacgca
     1201 gacgaccctg ccgccgtact gcgtgaacag ctgcaccgcg aactgggcca cgttcccgaa
     1261 gccctggacc gacgcggtca tcgacgccgg atcgagcccg agcaggcgca gcgcctcgcg
     1321 gacggtgtag atcacgccgt agcccgtcgc ctcggtgcgg ccgagagagc cgccgctgcc
     1381 gaccggcttc ccggtgatga agcccggttc cttctggccg tggaggacct cgatctcgtc
     1441 catcatccag agcatgtgct gcgggttcgt catcacgtcg ggcgcaggca cgtcctggag
     1501 gtagccgacg ttcttcgaca gctgccggac ccagccgcgg cagatggctt cctgctcgcg
     1561 caggctcagc tggtgcggat cgcagacgac gccgcccttg ccgccgccga gggggatgtc
     1621 caccacggcg cacttccagg tcatccaggt cgcgagggcg cggacggtgt cgagcgtctc
     1681 gttcgggtgg aagcggatgc cgcccttgtt ggggccgcgc gcgtcgttgt gctggacgcg
     1741 gaacccccgg aacaccctca ccgtcccgtc gtccatgcgc acgggaatgg cgaactggta
     1801 ctcgcgcagc ggccagcgca gcagctcgcg agtggcctgg tccaggccga ggagatcggc
     1861 caccctgtcg aactgcgcct gggccatctc gaaggggttg aacggcttgg tcacgacggt
     1921 cactccttcg gcgccggtgg cgccgagctg aagaggcgtg ccacccggcc tgcgcgccgt
     1981 caggcgtaca tcccgcgaac cctcgtcgcg gccgccacgc gggcgatggc taccacagtg
     2041 gccgccgtcc gcagatcggt ctcgtacttc cgcgctgcgc cgaacacggc ctcgaacgcg
     2101 gcgcccatcc gcgcgtcgag ccgctggtgc acgtcggctt cgctccaact gtcgccccgg
     2161 cggttctgct cccactcgag gtgcgccgcc gtggcgcgcc ccgcgccgga caggatttcc
     2221 ggcaccacga gcagaccgcg ttcctgcagc acccggcacg cctctgccgt cgtcgcgccg
     2281 accgacgcct cgacgacgag cggggcgcgc acggagccgg ccgccgcctc cgtcaccggg
     2341 ctcgcggcgg tgccggcgag gagaatgtcg gcctcgaggc cgagtacgtc gccaatcccc
     2401 agcgggcctc ctccagcaaa gccgtccacg gtccggctct gccgccggtg ttcgagcagg
     2461 gcgccaacat ccaggccccg cggctcgtac acgccgccgc gagcgtcagc ggccgcgatc
     2521 acgcgcgccc ccatgcgctc gaggaccacg gcggcctcct cgcagacgcc gtcgaatccc
     2581 aggacggcga cccgggcacc cgggagcgcg aagccgaccg atcgcgcggc ctgcctggca
     2641 gcgatcgcg
//
LOCUS       DAOSIF010000432         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_33806_length_2636_cov_3.920573, whole genome shotgun sequence.
ACCESSION   DAOSIF010000432 DAOSIF010000000
VERSION     DAOSIF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33806_length_2636_cov_3.920573"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLT35_12140"
     CDS             <1..336
                     /locus_tag="PLT35_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOG30021.1"
                     /translation="RVQASYADYVWGLPPEEAPLYPSLGISVREGDGESPLSIIDVQD
                     DSVARDAGLQAGDVLQTMDGTPVKDRETLNRLLADKRWADAVTLTVDRRGELLTITAV
                     FRRQPPPVK"
     gene            403..>2636
                     /gene="lon"
                     /locus_tag="PLT35_12145"
     CDS             403..>2636
                     /gene="lon"
                     /locus_tag="PLT35_12145"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG30022.1"
                     /translation="MSDPLLTPLPPLPPRLPVVPLLDTVVFPLTLQPLAVNRAVSIDS
                     IHRALGGDRLVVLVQQHTAADEPGSQDLRGTGTAGLIRQMARTPAALQVAVEGLVRVR
                     VSDAARAGAALDAAVEAIPDDEPRDDEIDAYVRTLRDLGERALAFSPAPAQDMKALLA
                     GIDRPLRLCYLVASLLDLGGEEKQRLLEEGRLVEKLRAVAAALARNLHLIELKGKIES
                     DARQEMNAAQRESFLRQQLKAIREELGESDGAGWRDLQERIEQADLPEHVRKAAEREA
                     RRLERAPQTSPEHQMIRTYLDWLLEVPWSVSTEDRLDPAEARRVLDEDHYDLDRVKDR
                     IVEYLAVRKLKGDMKGPVLCFAGPPGVGKTSLGQSIARSMNRRFVRIALGGVRDEAEI
                     RGHRRTYIGALPGRIVQGLKQAGSMNPVVMLDEIDKLTAGYQGDPAAALLEVLDPAQN
                     HAFRDHYLEVACDLSRVLFIANANQLGTVHRALLDRMEIIGLAGYTDLEKRHIARRFL
                     IPRQLAEHGLPAGSVEVSDEALGRLVREYTREAGVRGLEREIGAIARKVAARVATSGG
                     EGGAPPPGMVVAAVAAEDLQALVGPPRFRRDVQFRTSRFGVATGVAWTESGGDVLFIE
                     AMLLPAGHGQITLTGHLGSVMQESARAAVSHLRATAASLGLPPDLFKRHDLHVHVPAG
                     AIPKDGPSAGVTMATAILSAIRRQAVRPDLAMTGEITLSGAVLPVGGIREKVLAARRY
                     GVTT"
BASE COUNT          379 a          954 c          953 g          350 t
ORIGIN      
        1 cgggtgcagg cgtcatacgc cgactacgtc tgggggctgc ccccggagga ggcgccgctc
       61 tacccgtcgc tcggcatctc ggtgcgcgag ggcgacggcg agtcgccgct ctcgatcatc
      121 gacgtgcagg acgactcggt ggcccgcgac gcgggcctgc aggcgggcga cgtcctccag
      181 acgatggacg gcacgcccgt gaaggaccgg gagacgctga accggctcct ggcggacaaa
      241 cgctgggccg atgcggtgac gctcaccgtc gaccgccgcg gcgagctgct gacgatcacc
      301 gccgtgttca ggcgccagcc gccgcccgtg aagtagccag ggcggcgccc ccgccccggt
      361 gcgcccgcgt gagaggatga ggacgccgcc gcgcccacga ccatgtcgga ccccttgctg
      421 acgccgcttc cgcccctgcc gccccggctg cccgtcgtgc cgctgctcga caccgtggtg
      481 ttcccgctga cgctgcagcc gctcgccgtg aaccgcgccg tgtcgatcga ctccatccac
      541 cgcgcgctcg gcggcgaccg gctcgtcgtc ctggtgcagc agcacaccgc cgccgacgag
      601 cccggctcgc aggacctgcg cggaaccggc acggcgggcc tcatccgcca gatggccagg
      661 acgcccgccg ccctccaggt cgccgtcgag ggcctcgtcc gcgtgcgtgt ctcggatgcc
      721 gcccgggcgg gtgcggccct cgacgcggcc gtcgaggcca tcccggacga tgagccccgc
      781 gacgacgaaa tcgacgcgta cgtcaggacg ctgcgcgatt tgggcgagcg ggccctggcg
      841 ttctcgccgg cgccggccca ggacatgaag gccctgctcg cgggcatcga ccgtcccctg
      901 cggctgtgct acctcgtggc aagcctgctc gatctcggcg gcgaggagaa gcagcggctc
      961 ctcgaagagg gccgcctcgt cgaaaagctg cgggcggtgg cggcggccct cgcgcgcaac
     1021 ctgcacctga tcgagttgaa gggcaagatc gaatcggacg ctcgccagga gatgaacgcc
     1081 gcgcagcggg agtccttcct gcgccagcag ctgaaggcga tccgcgagga gcttggcgag
     1141 agcgacgggg ccggatggcg cgacctgcag gagcgaatcg agcaggcgga cctgcccgag
     1201 cacgttcgca aggcggccga gcgcgaggcc aggcgcctcg agcgcgcgcc gcagacctcg
     1261 cccgaacacc agatgatccg cacgtacctc gactggctgc tcgaggtgcc ctggagcgtc
     1321 tcgaccgaag accgcctcga tcctgccgag gcgcgccgcg tgctcgacga ggaccactac
     1381 gatctcgacc gcgtgaagga ccgcatcgtg gaatacctgg cggtgcggaa gctcaagggc
     1441 gacatgaagg gcccggtcct gtgcttcgcg gggccgccgg gcgtgggcaa gacctcgctc
     1501 gggcagtcca tcgcgcgatc gatgaaccgc cggttcgtgc ggatcgccct cggcggggtg
     1561 cgcgacgagg ccgagatccg cgggcaccgc cgcacctaca tcggcgccct tcccggccgc
     1621 atcgtccagg gcctcaagca ggcggggtcg atgaacccgg tggtgatgct ggacgaaatc
     1681 gacaagctga ccgccggtta ccagggcgat ccggcagcgg ccctgctgga ggtgctggat
     1741 cccgcccaga accacgcctt ccgcgaccac tacctcgaag tggcgtgcga cttgtcgcgc
     1801 gtgttgttca tcgccaacgc caaccagctc ggaacggtgc accgcgcact gctcgatcgg
     1861 atggagatca tcgggctggc gggctacacc gatctcgaga agcggcatat cgcgaggcgc
     1921 ttcctcatcc cgcgccagtt ggccgagcac gggctgccgg ccggctccgt cgaggtgtcg
     1981 gacgaggccc tcggccggct cgtccgcgag tacacgcggg aagccggcgt gcgcggactc
     2041 gagcgtgaga tcggcgcgat tgcgcggaag gtggcggccc gcgtcgccac gagcgggggc
     2101 gagggcggcg cgcccccgcc ggggatggtc gtggccgccg tcgcagccga ggacctgcag
     2161 gccctggtcg gccctccgcg gttccggcgc gacgtgcagt tccggacatc gcggttcggt
     2221 gtggccacgg gcgtcgcgtg gaccgagagc ggcggcgacg tgctgttcat cgaggccatg
     2281 ctgctgccgg cgggtcacgg ccagatcacg ctgacggggc atctcggcag cgtcatgcag
     2341 gagtcggccc gcgcggcggt gagccacctc cgggccaccg cggccagtct cggcctgccg
     2401 ccggatctgt tcaagcggca cgacctgcac gtgcacgtcc cggccggcgc catccccaag
     2461 gacggcccct ccgcgggcgt gaccatggcc acagccatcc tgtcggccat ccgccgccag
     2521 gccgtccggc ccgatctcgc gatgaccggc gagatcacgc tgagcggcgc ggtgttgccg
     2581 gtcgggggca tccgcgagaa ggtgctcgcg gcccgccgct acggcgtcac cacgtt
//
LOCUS       DAOSIF010000433         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_33950_length_2628_cov_3.028372, whole genome shotgun sequence.
ACCESSION   DAOSIF010000433 DAOSIF010000000
VERSION     DAOSIF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33950_length_2628_cov_3.028372"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLT35_12150"
     CDS             complement(<1..422)
                     /locus_tag="PLT35_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30023.1"
                     /translation="MTTTASTHPLEALAAAQRRRNASLLEASPLLAGRVEADVLPFSL
                     DPAAASLAPDEAAALAGLEKEAASIAIRALASLARAGDIDHLGGALELIPGLLMTLGV
                     VDYDRRFFTIEHGHTSIGYYAALAILGFLPESRVVEAFR"
     gene            612..1739
                     /locus_tag="PLT35_12155"
     CDS             612..1739
                     /locus_tag="PLT35_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG30024.1"
                     /translation="MPKALTAEDVRRAVLARAEIVRLLEHSRAEDRTAVRRRIVAYLE
                     ELTTTQRYAFYKRIPALLHPILRKIPRLVEGIDAAKDASRSGRLIYASNHRSHMDYLI
                     EPILLDEHGIRPPLIAAGFNLFGGPLGLLNRHVTGAIPIRRGTRDPVYLTTLKAYVAE
                     QLQHHDLFVYPEGGRSYSGELKPYKTGLLHAAMQAGVKDLLVVPTAISYDLVLEDRVI
                     ARQGVKKRQRPFTHEFAEMAALAIGYRTRSVTVFAPPIPAAPYDPESKKDVLDLAQRV
                     REVTGRIMKILPTAIVAHEMRQAITHASLESRVAARLDRLAEAGANLDVRDAKHVIED
                     GVSRLEHRGVIQSESGRIRVRDRRVLRYYGRSIAHFVHDTD"
     gene            1749..>2628
                     /locus_tag="PLT35_12160"
     CDS             1749..>2628
                     /locus_tag="PLT35_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOG30025.1"
                     /translation="MIDALSKSFFHALARSKGLERLASRYGMATPTSFARRFIAGESA
                     EEALAAARAIQARGMRLTLDQLGESIASLPEAEAAARAYEALIQKLVAAGIDRNISIK
                     PTQLGLDIDMAVCLGHITNLLDLARRHEAFVRIDMENSPCIQKTLDLFGAAWAQGYRN
                     TGVVLQSAILRSESDARVLSQMGARIRLVKGAYKEPPGIAYQAKADVDRAYVRIMQFL
                     LAEGHYPAIATHDPAMIAAARAFAADRGIPSDAFEFQMLFGIRRDLQAALVAEGYRVR
                     VYVPFGTQWFPYFMRRL"
BASE COUNT          418 a          942 c          906 g          362 t
ORIGIN      
        1 cggaacgcct caacgaccct ggactcgggc aggaagccga ggatggccag cgcggcgtag
       61 tagccgatgc tcgtgtggcc gtgctcgatg gtgaagaacc ggcgatcgta gtccacgacg
      121 ccgagcgtca tgagcaggcc cggtatcagc tcgagcgcgc cgccgaggtg gtcgatgtcg
      181 cccgcgcgcg cgagcgacgc cagcgcccgg atggcgatcg acgcggcctc cttttcgagc
      241 cccgcaagcg ccgccgcctc gtcgggggcg agcgaggccg ccgcggggtc gagcgagaac
      301 ggcagcacgt cggcttcgac gcgcccggcc agaagcggcg acgcctcgag gaggctcgcg
      361 ttgcgccggc gctgggcggc cgcgagcgcc tcgagcggat gcgtggacgc ggtggtggtc
      421 atggcggctc tcgtcatcgg tgagggtcgg gcagcgggtc acggcagtct agcacaccgg
      481 ttccgccgcc tcgggctagc ctgcgccacc gcccgcgccg cgggaaccgc gcccgcagtt
      541 cgcgcgcgga cctataatgg ggccgtgcag ccggccaggg tcctgggggg cggccgcgca
      601 acaggaggcc catgccgaag gccctcacgg cggaggacgt caggcgcgcg gtgctggcgc
      661 gagccgagat tgtcagactg ctcgagcaca gccgggccga ggaccgcacg gcggtccgcc
      721 gccggatcgt cgcctacctc gaagaactga cgacgacgca gcgttacgcg ttctacaagc
      781 gcatccccgc gctgctgcac ccgatcctcc ggaagatccc tcgcctcgta gagggcatcg
      841 atgctgccaa ggacgccagc cgatccggcc gcctcatcta cgcgtcgaac caccgcagcc
      901 acatggatta cctcatcgag ccgatcctgc tcgacgagca cggcatccgg ccgccgctca
      961 tcgcggcggg cttcaacctg ttcggcggcc ccctcgggct gctgaaccgc cacgtgacgg
     1021 gcgcaatccc gatccgccgg ggcaccaggg acccggtgta cctgacgacg ctcaaggcgt
     1081 acgtcgcgga gcagctccag caccacgacc tgttcgtgta cccggagggc gggcgcagct
     1141 acagcggcga gctgaagccg tacaagacgg gcctgctgca cgccgccatg caggccggcg
     1201 tgaaggacct gctggtcgtg cccaccgcca tttcgtacga cctggtcctc gaagaccgcg
     1261 tcatcgcgcg gcagggcgtg aagaagcgcc agcggccctt cacgcacgag ttcgccgaga
     1321 tggccgccct ggcgatcggg taccgcaccc gatccgtcac cgtcttcgcg ccccccatcc
     1381 cggccgcgcc gtacgatccc gaatcgaaga aggacgttct ggacctcgcg cagcgggtgc
     1441 gcgaggtgac aggcaggatc atgaagatcc tgccgaccgc catcgtcgcc cacgagatgc
     1501 gccaggcgat cacgcacgcg agcctggagt cgcgcgtcgc cgccaggctg gaccggctcg
     1561 ccgaagccgg cgcgaacctg gacgtccgcg acgcgaagca tgtgatcgag gacggcgtgt
     1621 cgcggctcga gcaccggggc gtcatccagt ccgagagcgg caggattcgc gtgcgcgatc
     1681 gccgggtgct ccgctactac gggcgctcca tcgcgcattt cgtccacgac accgactagg
     1741 acgccttcat gatcgacgcg ctgtcgaagt cctttttcca cgcccttgcc cggagcaagg
     1801 gcctcgagcg cctggcgtcc cgctacggga tggccacgcc cacgagcttc gcgcgccggt
     1861 tcatcgccgg cgagagcgcc gaggaggcgc tggcggccgc ccgcgcgatc caggcgcgag
     1921 gcatgaggct caccctggac cagctcggcg agagcatcgc gtcgctgccg gaggccgagg
     1981 cggcggcgcg cgcctacgag gccctcatcc agaagttggt ggcagccggc atcgaccgca
     2041 acatctcgat caagccgacg cagctcggcc tcgacatcga catggccgtg tgcctcgggc
     2101 acatcacgaa cctcctggac ctcgcccggc ggcacgaggc cttcgtgcgc atcgacatgg
     2161 agaactcgcc ctgcatccag aagacgctcg acctgttcgg cgcggcctgg gcgcagggct
     2221 atcggaatac cggcgtcgtg ctgcagtcgg ccatcctccg aagcgaatcc gacgccaggg
     2281 tcctgtcgca gatgggcgcg aggatccggc tcgtgaaggg cgcctacaag gagccgcccg
     2341 gcatcgccta ccaggcgaag gcggacgtgg atcgggccta cgtccggatc atgcagttcc
     2401 tgctggcgga ggggcactac cccgccatcg cgacccacga cccggccatg atcgccgccg
     2461 ccagggcgtt cgccgccgat cggggcattc ccagcgacgc cttcgagttc cagatgctgt
     2521 tcggcatccg gcgggacttg caggcggcgc tcgtcgccga gggataccgc gtacgcgtct
     2581 acgtcccgtt cggcacgcag tggttcccgt acttcatgcg ccggctcg
//
LOCUS       DAOSIF010000434         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34039_length_2622_cov_4.026880, whole genome shotgun sequence.
ACCESSION   DAOSIF010000434 DAOSIF010000000
VERSION     DAOSIF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34039_length_2622_cov_4.026880"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2622
                     /locus_tag="PLT35_12165"
     CDS             <1..>2622
                     /locus_tag="PLT35_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG30026.1"
                     /translation="DGIVKGAGNEPFAGALVSSDVLDVVSLSASTGAYFAAAPGGPVT
                     LTALDLVKNDSGSAATFLSAGLVLPVTLQIAEQPPRVVSVAPANGARGVPLTDPVVVT
                     FSEPIDPASVAGANLANVLLADSAGARVEGAAALSAGNTILTFRPVSALAANTRYTLT
                     LSAAITDVSGYALTPAFTSYFDSLDTTPPPMPPAGTISASIPAGGLSTVAATQGTVSP
                     TDAVYIKNLTTGISVPVPQALYQANGSFEFAVPASVTDRLQLRIVDEAGNETVVDLER
                     FQQTNADGSLSVAVDGEGADIVGPGGAQVEVPPNAFPDGAVVTVKGLALAEIPVQLSG
                     TDLERFAMQGALTLDFGGQVPARHVNVSIPADGDEPADTVWVVSRLVELRGETRMAVV
                     DTAKLRDGRIWTASPPFPGVTSGGTYVFVQSKGPMGVAHGRVLSMPGSANFIEASLQR
                     MSSDLGTIVTMLPFVVSSDYADAWGYPFPAGRVTLSQNRVRLRVEPTIVAPMHRELRV
                     RNTVTSQVESYPIVPVDYRVQVAGAIDDGFDIRVRGPLGEHKVTVARLTSGAPGSVVI
                     RLDADAIEVPVTQVYVKNLVSAAESTFDFPLPDVVASVPGGLGDAREVDLVQVSGEFW
                     RLASSEYHVLPSLAGDTGNLVLRIAGGALTGGGEVKSLRLAGQGGFDLEVPFAPGALE
                     QDFRWAFDGDAAQLYVLNVDYGSGSPIPIAIPAFTLSVTSVTTNQVTRTISAFVPPPD
                     EPMDLGTVTDDPNVPYIVRGPSQTGAFDPLSPLTLTFSEPMDPASLKQFITLLDGQGR
                     AVAGEVRVSGGNMVATFVPAQPLRMGRTYTLKVQGGTPATPAERAQGIVPAGAVDRGG
                     NPVATTAITITTFAPRSI"
BASE COUNT          365 a          913 c          948 g          396 t
ORIGIN      
        1 gacggcatcg tgaagggagc cggcaacgag ccattcgccg gcgcgctggt gtcgagtgac
       61 gtcctcgacg tcgtgtcgct gtccgcgtcg acgggcgcgt atttcgccgc cgctccgggc
      121 ggtcccgtca cgctgaccgc gctcgatctc gtcaagaacg actccggatc ggcggcgacg
      181 ttcctctcgg ccgggctcgt gctgcctgtc accctgcaga tcgcggagca gccgccgagg
      241 gtcgtgagcg tggcgccggc caacggcgcg cgcggcgtgc cgttgaccga ccccgtggtg
      301 gtgacattct cggagcccat cgacccggcg tcggtggcgg gagcgaacct cgcgaacgtg
      361 ctgctcgcgg actccgccgg cgcgcgcgtc gaaggggctg cggcgctgtc ggccggcaac
      421 accatcctca cgttccgccc cgtctcggcg ctcgcggcca acacgcggta cacgctcacg
      481 ctctccgccg cgatcacgga cgtgtccggc tacgcgctca cgccggcgtt cacgtcgtac
      541 ttcgacagcc tcgacacgac gccgcctccg atgccgccgg cgggcacgat ctccgcttcg
      601 attccagccg gcggcctcag cacggtcgcg gcaacccagg gaacggtgtc gccgacggac
      661 gcggtctaca tcaagaacct gactacgggt atctcggtcc cggtgccgca ggcgctgtat
      721 caggcgaacg ggagcttcga gttcgccgtg ccggcctccg tgacagaccg gctgcagctg
      781 cgcatcgttg atgaggcggg caacgagacg gtggtggacc tggagcgctt ccagcagacg
      841 aacgccgacg gcagcctgtc cgtcgcggtg gacggcgagg gcgccgacat cgtcgggccc
      901 ggcggggcgc aggtggaagt gccgcccaac gcattcccgg acggcgccgt ggtcaccgtg
      961 aagggactcg cgctcgccga gatcccggtt cagctctccg ggaccgacct tgagcgcttc
     1021 gcgatgcagg gggcgctgac gctggacttc ggcggccagg tcccggcgcg ccacgtgaac
     1081 gtgtcgatcc cggccgacgg agacgagccg gcggacacgg tgtgggtggt gagccggctc
     1141 gtcgagctgc gcggcgagac gcggatggcc gtcgtggaca ccgcgaagct gcgggacggc
     1201 cggatctgga cggcgtcgcc cccgttcccg ggcgtgacca gcggcgggac gtacgttttc
     1261 gtccagtcga aggggccgat gggggtggcg cacggccgcg tgctctcgat gccgggcagc
     1321 gcgaacttca tcgaggcgtc gctccagagg atgtcgtcgg acctcggcac tatcgtgacc
     1381 atgctgccgt tcgtcgtgag cagcgactac gccgacgcgt ggggctatcc cttcccggcc
     1441 ggtcgcgtca cgctgtcgca gaaccgggtg cgcctgcgcg tcgagccgac gatcgtcgcg
     1501 cccatgcacc gcgagctgcg tgtgcggaac accgtcacct cgcaggtgga gtcctatccc
     1561 atcgtgcccg tcgactaccg ggtgcaggtc gcgggcgcga tcgatgacgg gttcgacatc
     1621 agggtcaggg gaccgctcgg cgagcacaag gtcaccgtgg cccgcctgac gtcgggcgcg
     1681 ccgggctcgg tcgtgatcag gctcgacgcc gacgcgatcg aggtgccggt gacgcaggtc
     1741 tacgtcaaga acctggtcag cgcggccgag tccaccttcg acttccctct tcccgatgtc
     1801 gtcgcgagcg taccgggcgg cctcggcgac gctcgggagg tcgatctggt ccaggtgtcc
     1861 ggcgagttct ggcgcctcgc ctcgagcgag taccacgtgc tgccctcgct ggcgggggac
     1921 accggcaacc tggtgctgcg catcgccggc ggcgcgctga cgggcggcgg cgaggtcaag
     1981 tcgcttcggc tcgccgggca gggcggcttc gacctcgagg tgcccttcgc gcccggcgcg
     2041 ctggagcagg acttccggtg ggcgttcgac ggcgatgcgg cgcagctcta tgtgctcaac
     2101 gtggactacg ggtcgggcag cccgatcccg atagccatcc cggcgttcac gctcagcgtc
     2161 acgagcgtca cgacgaacca ggtgacgcgc acgatcagcg ccttcgtgcc gccgccggac
     2221 gagccgatgg atctcggcac ggtcaccgac gacccgaacg ttccgtacat cgtgcgcggg
     2281 ccgtcgcaga ccggcgcctt cgacccgctg agccccttga cgctcacgtt ctcggagccg
     2341 atggacccgg cgagcctgaa gcagttcatc accctgctcg acggccaggg gcgggccgtc
     2401 gccggtgagg tccgcgtctc cggcgggaac atggtggcga cgttcgtgcc ggcgcagccg
     2461 ctgcggatgg gaaggaccta tacgctgaag gtccagggag gcacgccggc gacgccggcc
     2521 gagcgcgcgc agggcatcgt gccggcgggc gcggtcgatc ggggcggcaa tcccgtggcc
     2581 acgaccgcga tcacgatcac cacgttcgcc cccaggtcga tc
//
LOCUS       DAOSIF010000435         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34055_length_2621_cov_3.998051, whole genome shotgun sequence.
ACCESSION   DAOSIF010000435 DAOSIF010000000
VERSION     DAOSIF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34055_length_2621_cov_3.998051"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLT35_12170"
     CDS             <1..669
                     /locus_tag="PLT35_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG30027.1"
                     /translation="EDEPKVAGALREGMEGDDYEVAVATTGEDGFFLVSTQPFDLVVL
                     DWMLPGRSGFEILTGMRQRGIDTPVLILTARDALEDRVAGLDAGADDYLVKPFALSEL
                     LARVRALLRRTRPDKTASLKAADLEVDPVTREVTRGGEKLALTAREFELLEYLVRHKG
                     VIVSREMLARDVWKEPFRGTPLDNVIDVHITHLRKKVDQGFAVKLLHTVRGVGFVVKE
                     GGAQ"
     gene            666..2114
                     /locus_tag="PLT35_12175"
     CDS             666..2114
                     /locus_tag="PLT35_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG30028.1"
                     /translation="MRRKATSIRARLAVVFALTFVCLLTIYAFGVLGLLRQALYRELD
                     RQLESDFHVGEEMMENAERSGGRLRFEPMPPVPRPPDDAPQTWRWLEIWSPEGQLLYG
                     NPEDPRLVRYLPSAPAGIPVGPLSVDFPDGASVRVLSRPLVVLDTPVIIRVVRSEEGL
                     EHELSEYLQVLLIALPVAALLAGLAGYAVTKPALAPLVTMAERAQLITAENLGHRLPV
                     GNPDDELGRLARIFNDTLSRLEQAFQRLRRFTADASHELRTPLTIMRSVGEVGLQGER
                     LPGEYREIIGSLLEEADRLSQVVQSLLTLSRADAGQTKLAFERLELGQLCQEVCDLLD
                     VLAEEKDVTVTLVPRTQAFVMADRVTLREVFINLLDNAVKYSPPRTVVRVVVARSGRD
                     AVVEVADQGPGISAEDVARIFDRFFTVNSARNRPLGGSGLGLSIAQWAVQANGGRLTV
                     ESEEGVGSTFRVVLPAVDAPVVATAVAARVAG"
     gene            complement(2355..>2621)
                     /locus_tag="PLT35_12180"
     CDS             complement(2355..>2621)
                     /locus_tag="PLT35_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30029.1"
                     /translation="DETRHRIVTCNGADSSMTVIRQNGPDSYQLLGNIQTRPQARVMQ
                     LDEHTGRLFTVTADATFPAPGSDGAAVPPVFRPNSFTVLTYRPQ"
BASE COUNT          382 a          876 c          936 g          427 t
ORIGIN      
        1 gaggacgaac cgaaggtcgc cggggccctg cgggaaggca tggagggcga tgattacgaa
       61 gtggcggtgg cgaccacggg cgaagacggc ttcttcctgg tcagcacgca gccgttcgat
      121 ctggtcgtgc tcgactggat gctgcccggg cggtcgggct tcgagatcct caccggcatg
      181 cgccagcgcg gcatcgacac gccggtgctc atcctgacgg ctcgcgacgc gctcgaggat
      241 cgcgttgccg gcctcgatgc cggcgcggac gactacctcg tgaagccgtt cgcgctctcc
      301 gagcttctcg cgcgcgtccg ggccctgctg cggcggaccc ggcccgacaa gaccgcgagc
      361 ctcaaggcgg cggacctgga agtggacccc gtgacccggg aggtgacccg cggcggcgag
      421 aagctggcgc tgaccgcccg cgagttcgag ctgcttgaat acctggtgcg tcacaagggc
      481 gtgatcgtct cgcgcgagat gctcgcgcgg gatgtttgga aggagccgtt tcgaggcacg
      541 ccgctcgaca acgtcatcga cgtccacatt acgcacctgc gaaagaaggt cgaccaggga
      601 ttcgcggtca agctgctgca taccgtgcgg ggtgtcgggt tcgtggtgaa ggaaggcggc
      661 gctcaatgag gcggaaggca acgagcatcc gggcccgcct cgccgtcgtg ttcgcgctga
      721 ccttcgtgtg ccttctcacc atctatgcct tcggcgtgct cggcctgctc cggcaggcgc
      781 tctaccggga gctcgatcga cagctcgaat cggacttcca cgtcggcgag gagatgatgg
      841 agaacgccga acgcagcggc ggccgcctgc gtttcgagcc gatgccgccg gtgccacggc
      901 cgccagacga cgctccacag acctggcgct ggctggagat ctggagcccc gagggccagt
      961 tgctgtacgg caaccccgag gatcccaggc tcgtccggta tctgccctcg gcgcctgccg
     1021 gaatcccggt tgggcctctg tcggtggact tcccggacgg cgccagcgtg cgcgtgctca
     1081 gccggcccct ggtcgtcctg gacacgccgg tcatcattcg agtcgtgcga tccgaggagg
     1141 gtctggagca cgaactgagc gagtacctcc aggtcctcct gattgcgctc cccgtggccg
     1201 cgctgctcgc tggcctcgcc gggtacgccg tgaccaagcc cgcgctggcc cctctcgtca
     1261 cgatggctga gcgcgcgcag ctcatcacgg cggagaacct gggccaccgt cttcccgtcg
     1321 ggaacccgga cgacgagctc ggacgtctcg cgaggatctt caacgacacg ctgtcgaggc
     1381 tcgagcaggc gttccagagg ctgcggcgat tcacggcaga cgcgtcgcac gagctccgca
     1441 cgccgctcac gatcatgcgc agcgtgggcg aggtgggact tcaaggagag cgcctgccgg
     1501 gcgagtacag ggagatcatc ggcagccttc tcgaggaagc cgaccggctc tcgcaggttg
     1561 ttcagagcct gctcaccctc tcccgcgccg acgcgggaca gacgaaactc gccttcgagc
     1621 ggctggagct tggccagctc tgccaagagg tctgcgatct cctcgacgtc ctggccgagg
     1681 agaaggacgt gacggtcacg ctcgtgccgc ggacgcaggc gttcgtcatg gccgatcggg
     1741 tcacgttgcg cgaggtgttc atcaacctgc tggacaacgc ggtcaagtac agccctcctc
     1801 gaacggttgt ccgcgttgtc gtcgccaggt ccggacgcga cgccgtcgtg gaagtggccg
     1861 atcagggacc gggcatctct gctgaagacg tcgcgcggat cttcgatcgc ttcttcaccg
     1921 tgaacagtgc ccgcaatcgc ccgctcggcg gctcgggcct gggcctttcg atcgcccagt
     1981 gggccgtgca ggccaacggc ggccgcctga ccgtcgagag cgaagagggc gtgggcagca
     2041 cgttccgcgt cgtgctgccc gccgttgacg cccccgtcgt cgcgacggcg gtcgcagccc
     2101 gcgtcgccgg gtagcgcagg tcctcgccgc acccggcggg gctgcccgtt cgcccggctg
     2161 gcttcccgct ttcgagggtt ccctgcggcg ccttcagcgc ctgctggcgg cgcgcaccgg
     2221 gggtgcgtcg tcttccgccg gggtgggccg cggaatagcc gggccgcttc gcggggaccg
     2281 cccccatcga tggcgcagtg cggccactcg atggcgggcg gtccgggcag gcggcgccgc
     2341 gctgccagcc gtgactactg cggccggtac gtcagaaccg tgaacgagtt cgggcggaag
     2401 acaggcggca cggccgcgcc gtcggagccg ggggctggga acgtcgcgtc agccgtgacc
     2461 gtgaacagac gacccgtgtg ctcgtcgagc tgcatcacgc gggcctgggg ccgcgtctgg
     2521 atgttgccga gcagctggta ggagtcgggt ccgttctgcc ggatgacggt catcgaggaa
     2581 tcggcgccgt tgcaggtgac gatgcgatgg cgggtctcgt c
//
LOCUS       DAOSIF010000436         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34114_length_2618_cov_3.132267, whole genome shotgun sequence.
ACCESSION   DAOSIF010000436 DAOSIF010000000
VERSION     DAOSIF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34114_length_2618_cov_3.132267"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PLT35_12185"
     CDS             complement(<1..1333)
                     /locus_tag="PLT35_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG30030.1"
                     /translation="MRVPSIGPSVLLAVVAFVAALAAEASLRPSAPMLVTPLAASALA
                     LGFAYRAYRREEAARRAHDEQIERSTALQFATIEALALAIDARDHSSPSQLLREQRHA
                     AALAKALGMPPDAVEGVRTAALLHDIGKLAVPDHILSKSAPLTDDERSKMRTHAAVGA
                     ALIADVPFPYPVAEIVAAHHERWDGTGYPNGLRGADIPLGARVLAAVDHFAAVTSERS
                     LGGALPVEEAINVLWQEAGNALDPGVVARYAELLPALGKEDLEARLAASAPAGSRRAG
                     EAAARRPGPSELLEHIGEAHREMSGLLEVAATMGTGLGVAESMSVLASKLDRLVPFST
                     CALFLYDEPNGVARCRFATGAGEQVFPGLMVRDGQGLVGRAIATRACVVNGDPAAELD
                     ASKPGLAEAGLQSALVCPLVVAGSVRGALALYHTDPAYFTDNHRRLLTRVSG"
     gene            complement(1438..2151)
                     /locus_tag="PLT35_12190"
     CDS             complement(1438..2151)
                     /locus_tag="PLT35_12190"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOG30031.1"
                     /translation="MTGCPETAAVLCSAGLDSVVLAAHEATARDVQPVYVSSGLAWEP
                     AELARLECLGRSWAGAGRIRPPVVLDLTMRDVYPASHWAIRGAPPAYDTPDEDVYLAG
                     RNIVLLSKAAVYCAGARIHRLAIGLLAGNPFPDATPAFFGAMSGALSLGLAHRIDVVA
                     PFASLRKADVIRLGAALGAPLDLSLSCMSPQDGLHCGACSKCRERRDAFDEAGLADPA
                     RYAAPSPRRTQAVPPGRRP"
     gene            2179..2574
                     /locus_tag="PLT35_12195"
     CDS             2179..2574
                     /locus_tag="PLT35_12195"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOG30032.1"
                     /translation="MAKRIDFCYGHRLLDYDGVCKHPHGHNAVAEVTLRSGELDGRCM
                     VCDFGDIKRVVKAWIDQTIDHKMILRRDDPLVKPLQELGEPVFVVDDNPTAESIAKLI
                     FDYACSQGFAVESVRVWETPTSHATFHGD"
BASE COUNT          385 a          931 c          972 g          330 t
ORIGIN      
        1 ggccggagac gcgcgtcagc aggcgccggt ggttgtcggt gaagtaggcc gggtcggtgt
       61 ggtacagcgc cagcgcgccc cggaccgagc cggcgaccac gagcgggcag acgagcgccg
      121 actggagccc ggcttcggcc aggcccggct tcgaggcgtc gagttccgcg gccggatcgc
      181 cgttgacgac gcacgcgcgg gtggcgatgg ccctgcccac caggccctgg ccgtcgcgca
      241 ccatcaggcc cgggaacacc tgctcgccgg cgcccgtcgc gaagcggcag cgcgccacgc
      301 cgttcggctc gtcgtacagg aacagcgcgc aggtgctgaa cggcaccagc cggtccagct
      361 tcgacgccag gacggacatc gattccgcca cgccgagccc cgtccccatc gtggccgcca
      421 cctcgagcag gccgctcatc tcgcggtgcg cctcgccgat gtgctcgagc agctccgacg
      481 gccctggccg gcgggcggct gcctcgccgg cgcgccggga gccggctggc gccgaggcgg
      541 ccaggcgcgc ttcgagatcc tccttgccga gcgccggaag cagctcagcg tagcgggcca
      601 ccacgcccgg gtcgagggcg ttgccggcct cctgccacag cacgttgatg gcctcctcga
      661 ccggcagggc cccgcccagg gaccgctccg acgtgacggc ggcgaagtgg tccaccgccg
      721 cgagcacgcg cgcccccagc gggatgtcgg cgccgcgcaa cccgttcggg tagcccgttc
      781 cgtcccagcg ttcgtggtgg gcggcgacga tctcggccac ggggtacggg aacgggacgt
      841 cggcgatgag cgccgcgccg acagccgcgt gcgtgcgcat cttgctgcgc tcgtcgtcgg
      901 tgagcggagc cgacttcgag aggatgtgat cggggacggc cagcttgccg atgtcgtgga
      961 gcagggccgc cgtccggacg ccctcgacgg cgtcgggcgg catgccgagg gccttggcga
     1021 gcgccgccgc gtggcgctgc tcgcggagca gctgcgaagg cgacgagtgg tcgcgcgcgt
     1081 cgatggcgag ggcgagcgct tcgatggtgg cgaactgcaa ggccgtcgat cgctcgatct
     1141 gctcgtcgtg cgcgcggcgg gccgcctcct cccggcgata ggcgcgatag gcgaagccga
     1201 gcgccagcgc cgacgcggcc aggggcgtga ccagcatcgg ggcggacggc cggagcgacg
     1261 cctctgccgc aagggcggct acgaaggcca cgacggcaag cagcaccgat gggccgattg
     1321 aaggcacgcg catgtcagga ctgcggctcg agcagcgatt ttaccagcgg aacggcgttc
     1381 tgcgtccccc tgcgccacag gggccgcgcg ggagccggct ggccggctcc gcggcgatca
     1441 cggccgccgg cccggaggaa cggcctgcgt ccgccgcgga gagggcgcgg cgtagcgcgc
     1501 agggtcggcc agtcctgcct cgtcgaaggc gtcgcggcgc tcgcggcact tgctgcacgc
     1561 gccgcagtgc agcccgtcct gcgggctcat gcaggacagc gacaggtcga gaggggcgcc
     1621 aagggccgcc ccgagccgga tcacgtcggc cttgcggagc gaggcgaagg gcgcgaccac
     1681 gtcgatgcgg tgcgcgaggc cgagcgagag cgcgccggac atcgcgccga agaacgcggg
     1741 ggtggcgtcc ggaaacgggt tgcccgccag gaggccgatg gccagccggt ggatgcgcgc
     1801 gcccgcgcag tacacggcgg ccttcgagag cagcacgatg ttccgcccgg cgagatacac
     1861 gtcctcgtcc ggcgtgtcgt acgcgggcgg cgcgccgcgg atggcccagt gcgacgcggg
     1921 gtacacatcc ctcatcgtca ggtccagaac cacgggcggg cgaatgcggc ccgcgcccgc
     1981 ccacgaccgg ccgaggcatt cgaggcgcgc cagctcggcc ggttcccacg cgaggccgga
     2041 cgacacgtaa accggctgga cgtcgcgggc cgtcgcctcg tgggcggcca gcacgacgct
     2101 gtccagtccg gccgagcaga gcacggcggc ggtttcgggg catcccgtca tcggctgtag
     2161 aataaaccca tgtactcggt ggcgaagcgc attgacttct gctacgggca ccgcctgctc
     2221 gactacgacg gcgtgtgcaa gcacccccac gggcacaacg ccgtcgccga ggtcacgctg
     2281 cgcagcggcg agctcgacgg ccgctgcatg gtgtgcgact tcggcgacat caagcgcgtg
     2341 gtcaaggcct ggatagacca gaccatcgat cacaagatga tcctccgccg ggacgacccc
     2401 ctggtgaagc cgctgcagga gctgggcgag cccgtcttcg tggtggacga caaccccacg
     2461 gccgagagca tcgcgaagct catcttcgac tacgcctgct cgcagggctt cgccgtcgaa
     2521 tccgtccgcg tctgggagac accgacctcg cacgccacct tccacggcga ctgacgccgc
     2581 cgcaggactc tcatgcttct cgtttttgat ctcgacgg
//
LOCUS       DAOSIF010000437         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34197_length_2613_cov_3.729867, whole genome shotgun sequence.
ACCESSION   DAOSIF010000437 DAOSIF010000000
VERSION     DAOSIF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34197_length_2613_cov_3.729867"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /gene="trmD"
                     /locus_tag="PLT35_12200"
     CDS             complement(<1..498)
                     /gene="trmD"
                     /locus_tag="PLT35_12200"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG30033.1"
                     /translation="MRIDLVTIFPRACEAALGEGIVRRAIDRGLADVRVHDLRDHTAD
                     RHRTVDDVSYGGGPGMVLKPEPLFKAVEAITAEGPVDAVVLTSPQGRRFTQAEAARLA
                     ALGHIVLLCGRYEGVDERVRTALATEALSIGDYVLSGGELPALVIVDAVVRLLPGAVG
                     DEQSVE"
     gene            complement(495..1064)
                     /gene="rimM"
                     /locus_tag="PLT35_12205"
     CDS             complement(495..1064)
                     /gene="rimM"
                     /locus_tag="PLT35_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOG30034.1"
                     /translation="MSADWDDLVLVARVARPHGRRGEVILNPETDFPEIRFAPGAAVL
                     VRRGASPERLIVRSVWFMKARPVVGFEGFDSIDDAETLGGRELRIPAEQLAALPPGMF
                     YRHDLVGCLVETTGGEAVGEVAGVEGDGAASRLVVRTPRGEALVPLAAELCPVVDPQA
                     RRIVIDAPDGLLALNETARSRRERRERRP"
     gene            complement(1065..1319)
                     /locus_tag="PLT35_12210"
     CDS             complement(1065..1319)
                     /locus_tag="PLT35_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOG30035.1"
                     /translation="MSRARDVAETVTRALVDRPAAVRVTESAHRDGAIVEVVVAGDDL
                     GRVIGRQGRTAGAIRTLVAAAAEREGMRASVEFRDEHGQR"
     gene            complement(1316..1594)
                     /gene="rpsP"
                     /locus_tag="PLT35_12215"
     CDS             complement(1316..1594)
                     /gene="rpsP"
                     /locus_tag="PLT35_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG30036.1"
                     /translation="MLVIRLSRIGAKKQPTYRVVVAEKRTPRDSREVERLGYYNPRTR
                     PATLKVDRERVAYWLSAGAQPSATVKSLLKAHKEEAAEASAPVAALGR"
     gene            complement(1638..>2613)
                     /locus_tag="PLT35_12220"
     CDS             complement(1638..>2613)
                     /locus_tag="PLT35_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOG30037.1"
                     /translation="LLVSTDVRRPAAIEQLSVLGRQAGIRVFDPAGEADPVARASRAI
                     AEARTLGFDAVIVDTAGRLHVDSALMGELQAIRDAVHPDDALYVADAMTGQDAIRSAG
                     EFNRHIGVTGVILTKLDGDARGGAALSVVAVVGVPIAFVGVGERLDDLEAFQADRLVS
                     RLLGMGDVLSLIERAEHVVDRDEAARLERKLRADDFTLEDFLAQLKTLRKLGPLEQVL
                     AMLPGMGRLKDLGGASPDERQLNAVEAMISSMTPGERRSFRIIDGSRRRRIARGSGTA
                     VEDVNRLLRQFIQMRKMLKAVGAGGAGGGGRRRMRQAAAMLRGRGLGA"
BASE COUNT          358 a         1041 c          842 g          372 t
ORIGIN      
        1 ctcgaccgac tgctcgtcgc ccaccgcgcc gggcaggagg cgcaccacgg cgtccacgat
       61 caccagcgcc ggcagttcgc cgccggacag cacgtagtcc ccgatcgaca aggcctcggt
      121 cgccagcgcc gtgcggaccc tctcgtccac gccctcgtac cgcccgcaca gcagaacgat
      181 gtggccgagc gccgcgagcc gcgcggcttc ggcctgcgtg aaccgtcgcc cctggggcga
      241 ggtgaggacc acggcgtcaa cgggcccttc agccgtgatg gcttccaccg ccttgaacag
      301 cggctcgggc ttgagcacca tgcccggacc gccgccgtac gacacgtcgt ccaccgtgcg
      361 gtgacgatcc gccgtgtggt cccggagatc gtgcacgcgc acgtccgcca gcccccggtc
      421 gatcgcacgc cgcacgatgc cctcgccgag cgccgcttcg caggcgcggg ggaagatcgt
      481 gacgaggtcg atcctcacgg ccggcgctcc cgccgctccc ggcggctgcg ggccgtttcg
      541 ttgagggcca gcagcccgtc cggcgcgtcg ataacgatcc gccgcgcctg cggatcgaca
      601 acggggcaca gttcggcggc cagcggcacc agcgcttcgc cgcgaggcgt acgaaccacc
      661 agccggctcg ccgccccgtc gccttcgacg cccgccacct cgcccaccgc ctcaccgccc
      721 gtcgtctcga ccaggcatcc gaccagatcg tgccggtaga acatccccgg cggcagcgcc
      781 gccagctgtt cggccgggat ccggagttcg cgcccgccga gggtctcggc gtcgtcgatc
      841 gagtcgaacc cctcgaatcc gacgacgggc cgggccttca tgaaccacac cgatcgcacg
      901 atcaggcgct cgggcgacgc ccctcgccgc accagcacgg cggcgcccgg cgcgaacctg
      961 atctcaggga agtccgtctc cggattcagg atgacttccc cgcgccgccc atgggggcgc
     1021 gccacgcgcg ccaccaggac caggtcgtcc cagtctgcgc tcatctaccg ctggccgtgc
     1081 tcgtcgcgga actcgaccga cgcgcgcatg ccctcgcgct cggcggccgc cgcaaccagg
     1141 gtgcggatgg cgcccgccgt ccgtccctgg cgaccgatca cccgtccgag gtcatcgccc
     1201 gcaaccacca cctcgacgat tgcgccgtcg cggtgcgccg actcggtgac gcgcacggcg
     1261 gcgggccgat ccacgagcgc ccgcgtcacg gtttcggcga cgtccctggc ccggctcacc
     1321 ggccgagagc ggcaaccggg gcgctcgcct cggctgcctc ctccttgtgc gccttcagca
     1381 ggctcttgac cgtggcgctc ggctgggccc cggcgctcag ccagtaggcc acccgctcgc
     1441 gatccacctt gagcgtcgcg gggcgcgtcc tcgggttgta gtagcccagg cgctccacct
     1501 cgcggctgtc gcggggcgtt cgcttctcgg cgaccacgac gcggtaggtc ggctgcttct
     1561 tcgccccgat ccgggacaga cggattacca gcatgtcact ccttcaccgc cggcctcccg
     1621 gccggcacct cacgtcttca cgcccccagg ccgcggcctc tcagcatggc agcggcctgc
     1681 cgcatgcgcc gacggccgcc tcctccagcc cctccggcgc cgacggcctt cagcatcttg
     1741 cgcatctgta tgaactgcct cagcaggcga ttcacgtcct cgaccgccgt tccgctgccc
     1801 cgggcgatgc gccgccgccg gctgccgtcg atgatgcgga aactcctgcg ctctcccggc
     1861 gtcatcgagc tgatcatggc ctcgacggcg ttcagctgac gctcgtcggg gctggcccct
     1921 ccgagatcct tgagcctccc catgccgggc agcatcgcga gaacctgctc gagaggcccc
     1981 agcttccgca gcgtcttcaa ctgggcgagg aagtcctcga gcgtgaagtc gtcggcgcga
     2041 agcttgcgct cgagccgggc cgcctcgtcc cgatcaacca cgtgctcggc ccgctcgatg
     2101 agcgacagca cgtcccccat cccgaggagc cgcgacacaa gccggtcagc ctggaaggcc
     2161 tcgaggtcgt caaggcgctc ccccacgccc acgaacgcga tcggcacgcc gacgacagcc
     2221 acgaccgaga gggccgcgcc gccgcgggca tcgccatcca gcttcgtcag gatcacgccg
     2281 gtcacgccga tgtgccggtt gaactcgccg gcgcttctga tcgcgtcctg cccggtcatc
     2341 gcatccgcga cgtacagcgc atcgtcggga tggacggcgt cgcggatggc ttgcagttcc
     2401 cccatgagcg cgctgtcgac gtggagtcgg cccgcggtgt ctacgatcac ggcgtcgaac
     2461 cccagggtcc tcgcctcggc gatcgcccgc gacgcgcgcg cgacgggatc ggcctccccc
     2521 gccgggtcga agacgcggat gccggcctgc cggccgagca cgctcagttg ttcgatggcg
     2581 gcgggccgcc tgacgtcggt ggacacgagc agc
//
LOCUS       DAOSIF010000438         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34472_length_2598_cov_3.435706, whole genome shotgun sequence.
ACCESSION   DAOSIF010000438 DAOSIF010000000
VERSION     DAOSIF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34472_length_2598_cov_3.435706"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(526..1723)
                     /locus_tag="PLT35_12225"
                     /pseudo
     CDS             complement(526..1723)
                     /locus_tag="PLT35_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1733..>2598)
                     /locus_tag="PLT35_12230"
     CDS             complement(1733..>2598)
                     /locus_tag="PLT35_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOG30038.1"
                     /translation="VTELRDRMVGDMIARGLAPRTQEHYVRQVRLFARYFNRSPEYLG
                     AREVHRFRSHLLLERRLSASTVNQTVAALHFLYEVTLQRPWVTRHLEATRQRSGLPVV
                     LSPEEVALFFRSVRNIKHRTILLVVYGAGLRIAEATSLQVDDVDSSRAALRVRRGKGQ
                     KDRYALLSPRLLEALRAYWRLARPEGPALFPGRRPDRPISQDAVQHACARALRHCGLG
                     KHVTPHTLRHSFATHLLESGTDILTVQLLLGHTELSTTARYLHISTKALLATRSPLDQ
                     LDPAAFLTPTV"
BASE COUNT          478 a          691 c         1062 g          367 t
ORIGIN      
        1 ccgctggcgc atccagccgg caatgcggcc ttcaaacccg cgccagaaca ggcccctcca
       61 acgcgaaacg ccggcctcgt cggccggcgc atgctgctaa ccgcttcccg aggtcgggtc
      121 tgcggggaag atcaagtggc tccgcaggcg ggtcgctaag ccaacataga tctcagaacg
      181 cggcccaagg gaagaggttt ttgccgagtc tcgcgggaag cgcggtgagt cgtggcgtga
      241 agagtggagc gggcgggcga cagggaggcc cgcgagcact cggcaaagac ctggtttgaa
      301 ctaaaccgcc gtcgggacgt cggctatggg cgtgttgagg gtcggggagt gtcgtcggtg
      361 accgcgccgg tggagaacgg gaagggcgga acggtggacg gtgaccgggt tgcggcgtga
      421 aggcagacgt ctagccgatg cccccgaggg ggggcgagca gcgggagctg tgcgagatga
      481 ggatgggcgc gcgggtgtcg tctcatgagg agtcgcaggg gcagtcgaag atcgcggctt
      541 ggcggccgag ggtcggggga aggtcgaggc ggaggaagtg cgcggagccg caaagtgggc
      601 agaggcgtcg ggtgtcgacc gtgtccgtgt cggccggccg ggggctggcg ggagcctcga
      661 gagtgccggg agccttcagc tcggaggcag tggtgcgagg cagaaggctg cggcacaggg
      721 cgatgttcgc cgcgcgctgg cggttggcga agatcccgaa gtagcggatc tttgagaacc
      781 tcctgggcag gacgtggagg aggaagcggc ggatgaactc ggcggcaggc agagagagga
      841 cttttctgag atgcggctgg cggtagtcct tgtaccggaa gtcgacggag ccattgtcgg
      901 cggcgacgag gcggtcgttg gagatggcga cgcggtgggt gtaccggccg aggtaggcca
      961 gaacgtggtc gggtccgccg aagggtggct tggagtagac gacccactcc gtgcggcgca
     1021 gcgatgaaac gaaagcggcg aagtgagcag ccgggtcaga cggcgaggag gagtcgcccc
     1081 actgaaggcg gccttgcgcg tggaggtcgc tcaggccggc gaggaagagg cggcgaaaga
     1141 ggcgagacag gacgcggacg gggaggaaga agcgtggccg cgaggcgatc cagcgcttcc
     1201 tatcgggcga gaggccaccg gccggaacca cgcagtgcag gtggggatgg tggacgaggg
     1261 tctgccccca ggtatgaagg atcgccagga agccgatggc tgcgccgagg tgtcgagggt
     1321 cgcgcgcgat ggtcaggagg cttcgcgatg ccgcgcgaaa aagaaggtcg tagacggcgc
     1381 gcttgttggc gagggccagg tgggagagcg cgtcgggcaa cgtgaagacg atgtggaagt
     1441 acgggacggc gagcagctcg gcctgccgag cccggagcca tgcggaccgc gcggcggcct
     1501 gacacttcgg acagtggcga tcgcggcagg agttgtagga gatagtctga tggccgcagc
     1561 cggagcagat gtccacatgt cctccgagag ccgcggagcg acaggcttcg attgcagcca
     1621 tgacgcggcg ctgagcggag gagagcgacg gccccaaagc ccggcgggag tcggacccgt
     1681 agcgacgaaa gacgtcggcg acctcgacgg ccggcctgtt catggcgggc cgtcagacgg
     1741 taggagtgag gaaggcagcg gggtcgagct gatccagggg gctgcgggtg gccaggagag
     1801 ccttggtgga aatgtgaagg tagcgcgccg tcgtgctcag ctcggtgtgg ccgagaagga
     1861 gctggacggt gaggatgtcg gtgccggatt cgagaaggtg cgtggcgaac gaatggcgca
     1921 gggtgtgggg cgtgacgtgc ttgccgaggc cacaatgacg aagggcccgg gcgcaagcgt
     1981 gctggacggc atcctgggag atcggtcggt cgggccggcg gcccgggaac agggcgggac
     2041 cttctgggcg agccaggcgc caatacgctc gaagagcttc cagcaggcgg ggcgagagga
     2101 gtgcgtagcg gtccttctgt cccttgccgc gccggacgcg aagcgccgct ctgctgctat
     2161 cgacgtcgtc cacctgcaag ctggtcgcct cggcgatgcg cagtccggct ccatagacga
     2221 cgagcagaat cgtccggtgc ttgatgttgc gaacggatcg gaagaagagc gcgacctcct
     2281 ccgggctgag aacgacggga agtccagacc gctgccgggt agcctccaag tgtcgcgtga
     2341 cccaagggcg ctgaagcgtc acctcgtaga ggaagtgcag cgcggcgacg gtctggttga
     2401 cggtgctggc ggaaagtcgt ctttccagga gcagatggga ccggaatcga tggacctcgc
     2461 gggcgcccag gtattcggga gatcggttga agtagcgcgc gaagagccgg acctgccgga
     2521 cgtagtgctc ctgcgtcctc ggtgccagcc ctcgcgcgat catgtcgccg accattcgat
     2581 cgcgcagctc tgtcacgg
//
LOCUS       DAOSIF010000439         2589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34625_length_2589_cov_3.350829, whole genome shotgun sequence.
ACCESSION   DAOSIF010000439 DAOSIF010000000
VERSION     DAOSIF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34625_length_2589_cov_3.350829"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLT35_12235"
     CDS             complement(<1..1275)
                     /locus_tag="PLT35_12235"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG30039.1"
                     /translation="MVVMKFGGSSVATSAALARVCAIVAAERRRKIVVVSALGGVTDA
                     LLAAAAQAGAGDLAGALEGLRGIRERHEAIASGVRGQAERAALLQDLGAGWRDAEALL
                     RAASRLGSCSPAASDAIVAQGELASSRLAAAVLNDAGVRARWIDARQVVITDRRHQHA
                     APAAAETAARLTEVAAPLVGRGDVPVIGGFVGATADGVTTTLGRGGSDYSASLVGACL
                     GAAEIQIWTDVDGMLTADPRVFRRARPVARLSFREASALAGFGAKVLHPSAVQPAFAA
                     GIPVRILNARRPSAQGTVVTEEPIRRSAPAAGIACLGNLCAIDVPLPDGAGRLPALAS
                     AFEACALAQAVPALATVSDAGVSLVVGEGPEADRVAARLGPAAPTRRGGLALIAVVGD
                     RLASGVDVPRRLLAALDGIPVHLLSRTPGSIHV"
     gene            complement(1266..2336)
                     /gene="asd"
                     /locus_tag="PLT35_12240"
     CDS             complement(1266..2336)
                     /gene="asd"
                     /locus_tag="PLT35_12240"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG30040.1"
                     /translation="MATRSWDVGILGATGMVGQQLVRRLEGHPWFRLAWVAASERSEG
                     KRYGDTPWLLGGSVPAYARDLVLDKAEPGRAPRLVFSALDAGAAGPLERAFAEAGRLV
                     VSNARTHRLADDVPLLIPEVNAGHLDLLPTQRAARGWSGAIVTNPNCSTTFLAIVLAA
                     CRPFSPRRVIVTTFQAASGAGHPGVASWDLLGNVVPFIDGEEEKIERETRKILGTCTG
                     RSVMPAPVAVSAQAARVPVLDGHTEAISVEFDRVPEEAELAAALGGFTGLPPEAGLPS
                     APARPIVHLREPDRPQPRLDALREDGMAVCVGRVRRCPVLHYKLVALGHNTIRGAAGA
                     AILNAELLVARGWAGGGGRSWS"
BASE COUNT          357 a          986 c          933 g          313 t
ORIGIN      
        1 cacgtggatc gatccgggag tccgcgaaag caggtgcacc gggatgccgt cgagggcggc
       61 cagcaggcgg cgcggcacgt ccactcccga cgccaggcgg tcgccgacga cggcgatgag
      121 cgcaagtccg ccgcgccgcg tgggggcggc gggcccaagg cgcgccgcca cgcggtcggc
      181 ttcaggcccc tctcccacca cgagcgagac gcccgcgtcc gaaacggtcg cgagggccgg
      241 cacggcctgc gccagggcgc acgcctcgaa tgccgacgcc agcgccggca ggcggcccgc
      301 cccgtccggc aacggcacgt cgatcgcgca gaggttgccg aggcacgcaa tgcctgccgc
      361 cggggccgac ctccggatcg gctcctcggt gacgaccgtg ccctgcgccg aaggccggcg
      421 cgcgttcaga atgcgcacgg gaatcccggc cgcgaaggcg ggctgcacgg ccgacgggtg
      481 caggaccttc gcgccgaacc cggccagcgc cgacgcctcg cgaaacgaca gccgggccac
      541 cgggcgcgcg cggcgaaaga ctcgcgggtc cgccgtgagc atgccgtcga catcggtcca
      601 gatctggatt tcagcggccc cgaggcacgc gccgaccagc gaggccgagt agtccgaccc
      661 tccgcggccg agcgtggtcg tcacgccgtc ggcggtggcg ccgacgaagc cgccgatgac
      721 gggaacatcg ccccggccga cgagcggggc cgcgacctcg gtgagcctgg ctgcggtctc
      781 ggcggcggcc ggagccgcgt gctggtgccg gcggtcggtg atgacgactt gcctcgcgtc
      841 gatccagcgg gctcgcacgc cggcgtcgtt cagcacggcc gcggccagcc ggctgctggc
      901 cagttcgccc tgcgcgacga tggcgtctga ggcggccggc gagcagctcc cgagccgcga
      961 tgccgcccgc agcagcgcct cggcgtctcg ccagccggcg ccgaggtcct gcagcagcgc
     1021 agcgcgttcg gcctggccgc gcacgccgga ggcgatggcc tcgtggcgct cccggatgcc
     1081 gcgcaggccc tcgagcgcgc ccgcgagatc gccggcgccg gcctgcgcgg ccgcagccag
     1141 cagcgcgtcg gtgacgccgc cgagcgccga cacgacgacg atcttccgcc gccgctcggc
     1201 cgcgacgatc gcgcacacgc gcgcaagggc ggcggaggtg gcgacggacg agccgccgaa
     1261 cttcatcacg accatgaccg tccgcctccg cccgcccagc cgcgcgccac gagcagttcc
     1321 gcgttcagga tcgcggcccc ggccgcgccg cggatcgtgt tgtggcccag ggcgaccagc
     1381 ttgtagtgca gcaccgggca gcgccgcacc cggcccacac agaccgccat cccgtcttcg
     1441 cgcagcgcat cgagtcgcgg ctgaggccgg tcgggttccc gcaggtggac gatcggcctg
     1501 gccggcgccg agggcaggcc cgcctccggc ggcaggccgg tgaacccgcc gagcgccgcc
     1561 gccagttccg cttcttccgg cacgcgatcg aactccaccg aaatggcttc ggtgtgcccg
     1621 tcgaggacgg gcaccctcgc cgcctgcgcg ctgacggcga caggcgccgg catcactgac
     1681 cggccggtgc acgtgccgag gatcttcctc gtctcgcgtt cgatcttctc ttcctcaccg
     1741 tcgatgaatg ggacgacgtt gccgaggaga tcccaggagg ccacgcccgg atggccggcg
     1801 cccgatgccg cctggaaagt cgtgacgatc acgcggcgcg gcgagaacgg ccggcaggcg
     1861 gccagcacga tcgccaggaa ggtcgtggag cagttcgggt tggtgacaat cgccccgctc
     1921 caccctcgcg cggcgcgctg ggtcggcagc agatcgaggt gaccggcgtt cacctccgga
     1981 atgagaagcg gcacgtcgtc ggcgagccgg tgggtgcgcg cgttgctgac caccaggcgg
     2041 cccgcctcgg cgaaggctcg ctcgagcggg ccggcggcac cggcgtcgag ggcggagaag
     2101 accagcctcg gcgcccgtcc gggctcggcc ttgtcgagga cgagatcccg cgcgtacgcc
     2161 ggaacgctcc ctccgagcag ccagggcgtg tccccgtacc gcttgccctc ggaccgctcg
     2221 ctcgcggcca cccacgcgag cctgaaccac ggatggcctt cgagccgcct gacgagctgc
     2281 tggcccacca tccccgtcgc ccctagaatc ccgacgtccc agctcctcgt cgccatcgtc
     2341 cgttccgtcc cgtggccggc ggatgcgccg tccgtgtgcc cgaatccgtc cagccggcag
     2401 gcccgcgtcg ggagttgccg gcaaaaaaaa cccgccgact gcactgcagc tcggcgggtc
     2461 ctcctgggtc tctcgtgggt tcgtgatgct agggtccaga gacggctccc ccgccgatgc
     2521 cggtcattcg catcgccatc atccgcatcg cggcagccgg ggcggagtcg gtcggacgag
     2581 ccatgtgga
//
LOCUS       DAOSIF010000440         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34740_length_2581_cov_3.853523, whole genome shotgun sequence.
ACCESSION   DAOSIF010000440 DAOSIF010000000
VERSION     DAOSIF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34740_length_2581_cov_3.853523"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PLT35_12245"
     CDS             <1..1106
                     /locus_tag="PLT35_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOG30041.1"
                     /translation="LRIAPHFQGRREREAIERGYTLAGRPPRPNTRPLADFSNPAARA
                     FWQDGLSELLKLGVSAYALDPGEADIPDGGPFAIAGGRPLRELRNGYVVMYVEAAYEA
                     AKTHRGGDFLLVPRGAYTGSSKYAAFRGGDAGGTQEGLRASIIAAQRSAVMGYPFRGS
                     GACGDSRQPVEREVCARWIAFSALTPIMEVGPACNLAFWSQPGGAARDEELIAAWRLY
                     ARLHERLVEYGYRFVKEASSTGMPVVRPLFLADPGAPQAWTDWATYMYGTDLVVSPIW
                     ETGRRQAAVYLPSGSRWRDAWRRDQIHEGGRSVAVAADVHQIPLFVREGSGLDLGDLN
                     REWEDALAAARRRPDLKALDAALAEAWGRAPAR"
     gene            1138..1926
                     /locus_tag="PLT35_12250"
     CDS             1138..1926
                     /locus_tag="PLT35_12250"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOG30042.1"
                     /translation="MTISDRPVALVTGAARGIGRASALALGRAGFQVAVNDLGRPDDL
                     ARLGSLADELAGIGAENVACPLDIGDLDGHAALFEAVLARWGRIDCVVNNAGVSVRKR
                     GDLLDVEVESFDVNLSINTRAMFFMCQRAAKLMLAQGQIGSQHRSIINVTSCNVETLV
                     LSRGEYCVAKAASAMTTRLFALRLAEAGIGVYEIRPGIIETDMTRPVKAGYDARIAEG
                     LVPMRRWGQPEDIASAVVCMATGRLPYTVGQPFNIDGGLTIPHF"
     gene            1934..>2581
                     /locus_tag="PLT35_12255"
     CDS             1934..>2581
                     /locus_tag="PLT35_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG30043.1"
                     /translation="MKGRIPQPILDSLTDVPMPRGFARPAPAGTLRQGGFAIPRYRCG
                     ALVVGSGAAGLRAAVELRRRGVDVMVLTRSWYGGTSACSGSDKQTLHTAALERTGDDF
                     FKVAEALGAGGAMDADTAYVEAVGSVEALAGLKHLGLPLPEDRWGGVLRYQTDHDAAG
                     RATSCGPRTSRLMVRVLAQEATRLGVPGMGETTALRVLAAGAGRGRRASGLVAASP"
BASE COUNT          331 a          909 c         1003 g          338 t
ORIGIN      
        1 tgctgcggat cgcgccgcat ttccagggcc ggagggagcg cgaggcgatc gagagaggct
       61 acaccctcgc cggccggccc ccgcggccga acacccgccc gctcgccgac ttctccaacc
      121 cggcggcccg cgcgttctgg caggacgggc tgtcggagct cctgaagctc ggcgtctcgg
      181 cctacgcgct cgatccgggc gaggcggaca tccccgacgg cgggccgttc gcgatcgccg
      241 gcggccggcc gctgcgcgag ctgcgcaacg gctacgtcgt gatgtacgtc gaggccgcct
      301 acgaggcggc gaagacgcat cgcggcggcg acttcctgct ggtcccgcgc ggggcgtaca
      361 cgggcagctc gaagtacgcg gcgttccggg gcggggacgc cggcggcacg caggaagggc
      421 tgcgcgcctc gatcatcgcg gcgcagcgct cggcggtgat gggctacccg ttccggggct
      481 ccggcgcctg cggcgacagc cggcagcccg tggagcgcga ggtctgcgcg cgctggatcg
      541 ccttcagcgc gctcacgccg atcatggagg tcggcccggc ctgcaacctc gcgttctgga
      601 gccagcctgg cggcgcggcc cgcgacgagg agctcatcgc cgcgtggcgc ctctacgcgc
      661 gcctgcacga gcggctcgtc gagtacgggt accggttcgt gaaggaggcg tcgtcgacgg
      721 gcatgcccgt cgtgcggccg ctcttccttg ccgatcccgg cgcgccgcag gcctggaccg
      781 actgggccac ctacatgtac ggcacggacc tcgtggtgtc gccgatctgg gagacggggc
      841 ggcgccaggc cgccgtctac ctgccgtcgg gatcgcgctg gcgcgacgcg tggcgccgcg
      901 accagatcca cgagggaggg cggagcgtcg ccgtggcggc cgacgtccac cagatcccgc
      961 tgttcgtgcg cgagggaagc gggctcgatc tcggcgacct gaaccgcgag tgggaagacg
     1021 ccctggcggc ggccaggcgg cgcccggacc tgaaggcgct cgacgccgcg cttgccgagg
     1081 cgtgggggcg cgcccccgcc aggtaggctg gcggcggcgc cgcgaaggag aggcagcatg
     1141 accatctcgg accgtcccgt cgccctcgtc accggggcgg cgcggggcat cggccgggcc
     1201 tcggccctgg cgctcggccg cgcggggttc caggtcgcgg tcaacgatct cggcaggccc
     1261 gacgatctgg cgcggctcgg gtcgctggcg gatgaactgg ccggcatcgg ggccgaaaac
     1321 gtggcgtgcc ccctcgacat cggcgacctc gacgggcacg cggcgctctt cgaggccgtg
     1381 ctggcgcgct gggggcggat cgactgcgtc gtcaacaacg cgggcgtgtc cgtccggaag
     1441 cggggcgacc tgctcgacgt cgaggtggag agcttcgatg tcaacctctc catcaacacg
     1501 cgcgccatgt tcttcatgtg ccagcgcgcg gcgaagctga tgctggcgca ggggcagatc
     1561 ggatcgcagc accgcagcat catcaacgtc acgtcgtgca acgtcgagac gctggtgctc
     1621 tcgcgcggcg agtactgcgt ggcgaaggcg gcctcggcca tgaccacgag gctcttcgcg
     1681 ctccgcctgg ccgaggcggg catcggcgtg tacgagatcc ggccggggat catcgagacc
     1741 gacatgacgc ggccggtcaa ggcgggctac gacgcgcgca tcgccgaggg gctggtgccc
     1801 atgcgccgct ggggccagcc ggaggacatc gcgtcggccg tcgtgtgcat ggccaccggg
     1861 cggctgccct acaccgtggg ccagccgttc aacatcgacg gcgggctgac gatcccgcac
     1921 ttctgagggc gccgtgaagg gcaggattcc ccagccgatc ctcgactcgc tgacagacgt
     1981 gccgatgccc cgggggttcg cccggcccgc cccggccggg acgctcaggc agggcggctt
     2041 cgcaatcccg cggtaccgct gcggcgcgct ggtggtcggg agcggcgcgg cggggcttcg
     2101 ggcggccgtc gagctcaggc ggcgcggcgt cgacgtgatg gtgctgacca ggtcgtggta
     2161 cggcggcacg tcggcgtgct ccggatcgga caagcagacg ctgcacaccg cggcgctcga
     2221 gcgcacgggc gacgacttct tcaaggtcgc cgaggccctc ggcgcaggcg gcgcgatgga
     2281 cgccgacacg gcgtacgtcg aggccgtcgg atcggtcgag gcgctggccg gcctcaagca
     2341 cctcggtctc ccgcttcccg aggaccggtg gggaggcgtg ctgcgctacc agaccgatca
     2401 cgacgcggcg ggccgcgcca cgagctgcgg cccgcgcacg tcgcgcctga tggtgcgcgt
     2461 gctcgcgcag gaggcgacga ggctcggcgt cccgggcatg ggcgagacga cggccctgcg
     2521 ggtgctggcc gcgggcgccg gacggggccg ccgcgcgtcg ggcctggtgg cggccagccc
     2581 c
//
LOCUS       DAOSIF010000441         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34744_length_2581_cov_3.314727, whole genome shotgun sequence.
ACCESSION   DAOSIF010000441 DAOSIF010000000
VERSION     DAOSIF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34744_length_2581_cov_3.314727"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLT35_12260"
     CDS             <1..388
                     /locus_tag="PLT35_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG30044.1"
                     /translation="NPEKAAGRKVLVIDDEPVAANAVRRTLNRRGFRVDEAFTGHEAL
                     NRILNEMYDLVLLDMKMPDTNGLELLPTIKKHRPKLPVVMVTGYASIDTAVEAIQRGA
                     SDYVSKPFTPEELFTAASQAIRRAAV"
     gene            401..1624
                     /locus_tag="PLT35_12265"
     CDS             401..1624
                     /locus_tag="PLT35_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30045.1"
                     /translation="MGRLESGARLGLVAWAMAVTMARAVRCPNDFAEAHWLVDYRFGF
                     IRRGLAGSLYALPAEAGLVPQSAAAIAVLSFAIFGALLLLLLAASRRILEADANPALS
                     FSAAAVFATSPYVVMAGHFMGYLDHLVVLVAFAAAWLAARGRARPAAALAAVGVLLHE
                     SFLLVGLPLVVAAAALAPLTSMTWRARVLPLALPVGAALALFLSEALVLNPSVLRQQL
                     TARLAAFPFVAGDMHVFVPEWLTLGSWENLRGQSHAIWRRLGDPNLLRLMAPSALYLA
                     VYAARSTRGSRRPSPAALVAFATAAPLLLHLVAWDTARIWAFTIVAAFACAWLVASAA
                     GGDRDVGRTARPRTARLLVAVSALIVVANLLGRSPLMDGEVERFSTPLRLFLYSPFIA
                     GAALAIADGLCERRP"
     gene            2123..>2581
                     /locus_tag="PLT35_12270"
     CDS             2123..>2581
                     /locus_tag="PLT35_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG30046.1"
                     /translation="MTAFAGKLLEEQDGDVLREGVRMLAQALMETEVTALVGTERHER
                     SDGRTAYRNGPRMRTWDPRVGTVDVASPKVRPETYVPSLLQPRRRAEQARLAVGQEAD
                     VHGVSTRKVDDTVKALGLDRMSTWDVSRICGAWDPLVDAFRTRPITGEHPY"
BASE COUNT          347 a          909 c          894 g          431 t
ORIGIN      
        1 gaaccccgag aaggcggcgg gccgcaaggt gctggtcatc gacgacgagc ccgtggcggc
       61 caacgccgtg cgccgcaccc tcaaccgccg cggcttccgc gtggacgagg ccttcaccgg
      121 ccacgaggcc ttgaaccgca tcctcaacga gatgtacgac ctcgtgctgc tcgacatgaa
      181 gatgcccgac acgaacggcc tcgagctgct gccgaccatc aagaagcacc gccccaagct
      241 gcccgtcgtc atggtcaccg gctacgcgtc gatcgacacc gccgtcgagg ccatccagcg
      301 cggcgcgagc gactacgtct cgaagccctt cacgcccgag gagctgttca ccgcggcgag
      361 ccaggcgatc aggcgcgcgg cggtctagaa tcgtctgcct atgggcaggc tcgaatcggg
      421 cgccaggctg ggcctggtgg cgtgggccat ggccgtcacc atggcccgcg ccgtcaggtg
      481 ccccaacgat ttcgcggagg cgcactggct cgtcgactac cggttcggct tcatcaggcg
      541 cggcctcgcc ggctcgctct acgcgctgcc cgccgaggcc gggctcgtgc cccagtcggc
      601 cgcggccatc gccgttctct cgttcgcgat cttcggcgcg ctgctgctcc tgctcctcgc
      661 ggcctcgcgg cggatccttg aggcagacgc gaaccccgcc ctgtccttct ccgcggccgc
      721 cgtcttcgcc acctcgccct acgtcgtgat ggccgggcac ttcatgggct acctggatca
      781 cctggtcgtg ctcgtcgcct ttgccgcggc ctggcttgcc gctcgcgggc gcgcgcggcc
      841 ggccgcggcc ctagcggccg tcggcgtgct gctgcacgag agcttcctgc tcgtcgggtt
      901 gccgctggtc gtcgcagccg ctgccctcgc gccgctgacg tccatgacct ggcgcgcgcg
      961 cgttctccct ctggcgctgc ctgtcggggc tgcgctggcg ctgtttctct cggaggcgct
     1021 ggtcctgaac ccgtccgtgc tgagacagca gctcacggcc cgcctggctg cttttccgtt
     1081 cgtcgccggc gacatgcacg tcttcgtgcc cgagtggctg acgctcggat cgtgggagaa
     1141 cctccggggc cagtcgcacg ccatctggcg ccgcctgggc gaccccaacc tgctgcggct
     1201 gatggcgccg tccgcgctct acctggccgt ctacgccgcg cgctcgacgc gaggctcgcg
     1261 gcggccgtcg ccagccgctc tcgtcgcctt cgcgaccgcg gcgccgcttc tgctccacct
     1321 cgtggcctgg gacaccgccc gcatctgggc cttcaccatc gtcgcggcgt tcgcgtgcgc
     1381 gtggctcgtc gcttctgccg caggcggcga tcgggacgtc ggccgcacgg cccggccgcg
     1441 caccgcgcgt ctgcttgtcg ccgtgtcagc cctgattgtc gtggccaacc tgctggggcg
     1501 ttcgccgctc atggacggcg aagtcgagcg cttctcgact cctctgcgct tgttcctgta
     1561 ctccccgttc attgccggcg cggcgctggc catcgcagac ggcctgtgcg agaggcgtcc
     1621 gtgacgcctt tccgcttctg atcccgccct caatcgtccg gaagcagacc ctggcaattc
     1681 accgtcgatc tgccgccctg ccatcgtgcg cctgggtgga gaaagaaggc cggctgacca
     1741 cgctggccct gccggacgct gcggaggacg ttgtggggcg caccaaagcc gaacaagaac
     1801 aagagaccat tgtccggtgg gaccgcgagg accgccgggt cgagctgtac accctggacg
     1861 cggcccaagc ccggcagtgg gctcggctgg ggtatgacgt cagggtggtc gggagggacc
     1921 cggaaggtca gcccccggct ggacggcagt cggcccaacg gggtgcgtcc ggttccgacg
     1981 gttagagtcc ccggacgtgg tggaaactcg gtaggccgct gaactggaca aggcaagcgg
     2041 ccatcgcgca tcgttccgtg cgttcttctc gccaaagagg acctacacgg agggcgatat
     2101 cgatggcaag gcaaggattg atctgacggc gttcgctggc aagctcctcg aggagcagga
     2161 cggggacgtg ctgcgggaag gggtgcggat gctggcccag gcgctgatgg aaacggaggt
     2221 gacggcgctg gtcggcaccg aacgccacga gcgcagcgac ggccgaacgg cctatcgcaa
     2281 cgggccgcgg atgcggacgt gggatccgcg cgtcggcacg gtcgacgtgg cgagtccgaa
     2341 ggtccgcccg gagacatacg ttccctcgct gctgcagccg cgccgtcggg ccgaacaggc
     2401 gcggttggcc gtggggcagg aagccgacgt ccatggggtc tcgacccgga aggtcgatga
     2461 cacggtgaag gcgttgggct tagacaggat gtcgacgtgg gacgtgtcgc ggatctgtgg
     2521 cgcatgggat ccgttggtcg acgcgtttcg gacgcgcccg atcaccggcg agcatccgta
     2581 c
//
LOCUS       DAOSIF010000442         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34829_length_2576_cov_4.221341, whole genome shotgun sequence.
ACCESSION   DAOSIF010000442 DAOSIF010000000
VERSION     DAOSIF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34829_length_2576_cov_4.221341"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /gene="mtgA"
                     /locus_tag="PLT35_12275"
     CDS             complement(<1..729)
                     /gene="mtgA"
                     /locus_tag="PLT35_12275"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOG30047.1"
                     /translation="MAAAGRLLRWTLAAGAVAFAAAAYLYLSLPDVRPLRTKDPAVTA
                     FMELRARQAAAAGKKPRRVQRWVPYGRISASLKRAVLVAEDAAFFSHGGVDYDELQKS
                     LEKNVKEGRFARGGSTITQQLAKNLYLDPSKNPLRKIQELLIARRLEAELSKRRIFEI
                     YLNVIEWGEGVYGAEAAARAYFQKSAAELGPGESALMAAAIPSPRRSNPARPSKYLLR
                     RQQIILQRMGGAEPPAPPVQPAAPE"
     gene            complement(730..2184)
                     /locus_tag="PLT35_12280"
     CDS             complement(730..2184)
                     /locus_tag="PLT35_12280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG30048.1"
                     /translation="MKRHILIGGLAACSLLACATNPVTGDRQLNFMSEAQEIQLGRES
                     DPQLRAEMGVYPDQALQRYVESVGMRLAKSSERPDLPWQFAVVDVAAANAFAVPGGFI
                     YITRGLLAHLDTEAELAGVLGHEIGHVTARHSAAAYTRSTMATVGMLLTSLFAPKAKP
                     YMGAVEAGLSIALLKYDRTQELQADRLGAGYAAKVGWAPSGMSGVLETLGRISAASDS
                     RGLPNWLSTHPQPADRLTKLAETVRALESARPSSEWEVNRDAYWRHLDGLLYGDNPRE
                     GFLRGREFVHPDMRFTVTFPDGWRVQNGRQQVTSAPPGIDGVVILLQVVQNPQGASLE
                     RVAAAWMSQTGFQKVSGESVRLGGGVPAYHGVYSGQTESGTAVGVEAAHISHAGRVFL
                     LAGVASQARFAQVQPHFARTIRSYSPANEAEMARIKPNRIAFATVASGDTWQSIAQRT
                     GGLVPAGELAIVNGHAPDSRPPVGRRIKTVTQGS"
     gene            complement(2211..>2576)
                     /locus_tag="PLT35_12285"
     CDS             complement(2211..>2576)
                     /locus_tag="PLT35_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30049.1"
                     /translation="EDMADATVVMAGLGPLLLRASYREGIAASGDRVEWDVRRTGSLH
                     PVAMAYLTLVQAELAGLDPESRRRLLGDWIEPAWTVRQKAGGGIGDSRLPFPSPIGDR
                     RLVPSALLAAPSPGPRIPA"
BASE COUNT          366 a          932 c          868 g          410 t
ORIGIN      
        1 ttcgggggcg gcgggctgaa ccggaggggc cggcggttcg gcgccgccca tccgctggag
       61 gatgatctgc tggcgccgca gcaggtattt cgaggggcgg gcggggttcg accgcctcgg
      121 gctgggaatc gcggcagcca tcagtgccga ttcgcccggg ccgagttcgg cggccgactt
      181 ctggaagtac gcgcgggccg ccgcctcggc cccgtagacg ccctcgcccc attcgatcac
      241 gttgaggtag atctcgaaaa tccggcgttt ggacagctcc gcctcgagcc ggcgggcgat
      301 cagcagctcc tggatcttgc gcagggggtt cttggacggg tccaggtaca ggttcttggc
      361 gagctgctgc gtgatcgtgc tgccgccgcg cgcgaaccgg ccttccttca cgttcttctc
      421 cagcgacttc tgcagctcat cgtagtcgac gcccccgtgc gagaagaacg cggcgtcctc
      481 ggccaccagc accgcccgct tcaggctggc cgagatccgg ccgtagggga cccagcgctg
      541 aacccgcctg ggcttcttcc ctgccgccgc cgcctgccgg gcccgcagct ccatgaacgc
      601 ggtcacggcc gggtccttcg ttctcagcgg cctcacgtcc ggcagcgaca gatagaggta
      661 ggctgccgca gcgaacgcca ctgcccccgc ggccagtgtc cacctgagga gacgcccggc
      721 agccgccatc tagcttccct gagtgaccgt cttgatgcgc ctccccacgg gcggccggct
      781 gtccggggca tgcccgttca cgatggccag ttcgcccgca ggaaccaggc cacccgtgcg
      841 ctgggcgatg ctctgccacg tgtctccgct cgcgactgtc gcgaaggcga tgcggttggg
      901 cttgatgcgc gccatctcgg cctcgtttgc cgggctgtag gaccggatgg tccgcgcgaa
      961 atgcggctgc acctgagcga accgggcctg gctcgccacg ccggccagca agaacacgcg
     1021 gccggcgtgg ctgatgtgcg ccgcctccac gccgacagcc gtgccgctct ccgtctgtcc
     1081 gctgtagacc ccatgatagg ccggcacgcc gccgccgaga cgcaccgatt ccccgctgac
     1141 tttctggaac ccggtctgcg acatccacgc cgccgccacc cgctcgagcg aggcgccctg
     1201 cgggttctgc accacctgga gcaggatgac gacgccgtcg atgcccggag gcgccgacgt
     1261 cacctgctgc cggccattct ggacgcgcca gccgtccggg aatgtcacgg tgaaccgcat
     1321 gtcgggatga acgaactcgc gtccgcgcag gaatccctca cgcgggttgt cgccgtagag
     1381 cagcccgtcc aggtgccgcc agtacgcatc gcggttcacc tcccactcgg acgagggccg
     1441 cgccgactcg agcgcccgta ccgtctcggc gagcttcgtc aggcggtcgg cgggctgggg
     1501 atgcgtcgaa agccagttgg gcaggccgcg gctgtcgctc gcggcgctga tgcggccgag
     1561 ggtctcgagg acgccggaca tccccgacgg cgcccagccg accttcgcgg cgtatcccgc
     1621 gccgagacga tcggcctgca gctcctgggt ccggtcgtac ttcagaagcg cgatgctgag
     1681 gccagcctcg acggcgccca tgtacggctt ggccttgggc gcgaagaggc tcgtgagaag
     1741 catgccgact gtcgccatcg tcgagcgcgt gtaggccgcc gccgagtgcc gcgccgtgac
     1801 gtggccgatt tcatggccga gcacgccggc gagttcggcc tcggtgtcca gatgcgccag
     1861 cagtccgcgc gtgatgtaga tgaaccctcc cggcacggcg aacgcgttcg ccgccgccac
     1921 gtccaccacg gcgaactgcc acggcaggtc cggccgttcc gacgacttcg ccaggcgcat
     1981 gccgaccgat tccacgtacc gttgcaaggc ctgatcggga tagacgccca tctccgccct
     2041 gagttgcggg tcggactcgc gccccagctg aatctcctgc gcctcgctca tgaagttgag
     2101 ctggcggtcg ccggtcacgg ggttcgtcgc gcacgcgagc agggagcacg ccgccagccc
     2161 gccgatcagg atgtgccgct tcatggaacc tccgaggggc tgaggctatt ctaagctggg
     2221 attctgggcc cgggactcgg ggctgcgagc aaggcgctgg ggaccaggcg ccggtcgcca
     2281 atcggcgaag ggaagggaag ccgggaatcc ccgattcctc ctcccgcctt ctgcctcacg
     2341 gtccacgccg gctcgatcca gtcgccaagc agcctcctgc gcgattcggg atcgagtcct
     2401 gccagttccg cctgcacgag cgtgaggtag gccatcgcga cgggatgcag cgaccccgtc
     2461 cgccgcacgt cccactcgac ccggtcgccg cttgccgcga tgccctcgcg gtagcttgcg
     2521 cgcagcagca gcgggccgag gcccgccatc accaccgtcg cgtcggccat gtcttc
//
LOCUS       DAOSIF010000443         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34838_length_2576_cov_3.027767, whole genome shotgun sequence.
ACCESSION   DAOSIF010000443 DAOSIF010000000
VERSION     DAOSIF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34838_length_2576_cov_3.027767"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1563)
                     /locus_tag="PLT35_12290"
     CDS             complement(16..1563)
                     /locus_tag="PLT35_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOG30050.1"
                     /translation="MTKRLIAAACILALALPAAALAQPGATARLTILHTNDTHGHLLP
                     FSYPTIVPAGSELAGLAVRRDIGGIARRASLAQKIRTEQKGRGIPVWLVDVGDYSDGT
                     PFSTEYHGEADVAAMNAAGYDFATLGNHEFNNTFAQLQKLLAQTKHPVLLANAVERAT
                     RKPITQAWRVEKLGALRVGLFGLVSKDTASYPAAKEAVEILDPLATAPGIVETLRKQA
                     DIVILLSHCGNDADERIAAEVPGIDVIVGGHSHSRLPTGEFVWRGDELSQDDVNGTII
                     VQAHQWGGELGRLDLLFRKDAKGVWRVSRYRSRLLPITPDLPAEPMVAGVVEKFWQPI
                     APRFAGVVAVAAEDFSSRGDDDTPYHLVADAVRETFRTEIDLENTGGVRSPLVKGNVT
                     KADLVALDPFDNTVILFKATGAEIKSILTRYVPYASGLRYRIVEGQLVEATIGGAPID
                     DARIYTGSTNSYFAGFALRGITQENTTRTRLEVLTSYLQEKGTVTPAYDGRRVIIGRR
                     PREGTQP"
     gene            1635..>2576
                     /locus_tag="PLT35_12295"
     CDS             1635..>2576
                     /locus_tag="PLT35_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30051.1"
                     /translation="MLWIFIAYLLVTLTLGFWQGRRTKSQADFVLGSSRLPGWMLALS
                     ERATGESAWLLLGFTGFIYAHGLSGVWIGVGCLSGITTAWLVLARKFRSEAARYRAMT
                     MPEYFSAKFPEKANTIRVLSTLIIAFFFIFYVGAQFAGAGKTIQETFGLPAIWGQVLC
                     AAVIMTYASFGGFISVVAVDAFQAVLMILTLIVTPIVMLAKVLAGPVSIGQALEAAGP
                     GMSSLTGGAAGFAAGILIFNNFAWFFGYLGGQPQLNARFMGMRDDRQVRIGRNIAVAW
                     TVAAYTGAVMIGLCALALFGPKAIGDAELVLPFALTAL"
BASE COUNT          346 a          888 c          895 g          447 t
ORIGIN      
        1 cggccgtgca gcgcgctacg gctgcgtccc ttcccggggc cgccgtccga tgatgacgcg
       61 ccggccgtcg taggcgggcg tcaccgtgcc cttctcctgc aggtaggacg tcaggacctc
      121 caggcgcgtg cgcgtcgtgt tctcctgcgt gatgccgcgc agcgcgaacc ccgcgaagta
      181 cgagttcgtc gagccggtgt agatccgggc atcgtcgatg ggcgcgccgc cgatggtcgc
      241 ctcgaccagc tggccttcga cgatccggta gcgcagcccg gacgcgtagg gcacgtagcg
      301 ggtgaggatg ctcttgatct ccgcgcccgt cgccttgaag aggatcacgg tgttgtcgaa
      361 gggatccagc gccaccaggt cagccttcgt gacgttcccc ttgacgaggg gcgaccgcac
      421 gccccccgtg ttctccagat cgatctccgt ccggaaggtc tcccgcaccg cgtccgccac
      481 caggtggtag ggcgtgtcgt cgtcgccgcg gctgctgaag tcctcggccg cgaccgcgac
      541 gacgccggcg aaccgcggcg cgatcggctg ccagaacttc tcgacgacgc ccgccaccat
      601 cggctcggcg ggcaggtccg gcgtgatcgg gagcaggcgc gaccggtacc ggctcacgcg
      661 ccacacgccc ttggcgtctt tccggaacag caggtcgaga cggccgagct ctccgcccca
      721 ctggtgcgcc tggacgatga tcgtgccgtt cacgtcgtcc tggctcagct cgtcgccgcg
      781 ccagacgaac tcgcccgtgg gcaggcggga gtgcgagtgg ccgcccacga tgacgtcgat
      841 gccgggcact tccgccgcga tcctctcgtc cgcgtcgttg ccgcagtgcg agagcaggat
      901 gacgatgtcc gcctgtttcc gcagcgtctc cacgatcccc ggcgccgtcg ccagcggatc
      961 gagaatctcg accgcctcct tcgccgccgg ataggacgcg gtgtcctttg aaaccagccc
     1021 gaagagcccg acgcgcagcg cccccagctt ctccacgcgc caggcctggg tgatcggctt
     1081 gcgcgtggcc cgctcgacgg cgttggccag cagaaccggg tgcttggtct gcgccagcag
     1141 cttctggagc tgcgcgaagg tgttgttgaa ctcgtggttg ccgagcgtcg cgaagtcgta
     1201 gcccgccgcg ttcatggccg cgacgtccgc ttcgccgtgg tactccgtcg agaagggcgt
     1261 cccgtcggag tagtccccga cgtccaccag ccagacggga atgccgcggc ccttctgctc
     1321 ggtccggatc ttctgcgcga gcgacgcgcg ccgcgcgatg ccgccaatgt cccggcggac
     1381 cgcgaggccg gccagttccg agccggccgg cacgattgtc ggatagctga acggcagcag
     1441 gtggccgtgc gtgtcgttcg tgtggaggat cgtcaggcgc gccgtggctc ccggctgcgc
     1501 cagggcggca gccggaaggg ccagggccag gatgcaggcg gcggcaatca ggcgtttcgt
     1561 cacgtccagg tacctcgtgc cctattgtag tctgtgctag cgttgccgcg cccgcggcgg
     1621 gaggagaccg gccgatgctc tggatcttca tcgcgtacct gctcgtcacg ctgaccctgg
     1681 ggttctggca ggggcgccgg accaagtcgc aggccgactt cgtgctcggc agctcgcggc
     1741 tgccgggatg gatgctcgca ctgtcggagc gggcaacggg agagtcggcc tggctcctgc
     1801 tgggcttcac cgggttcatc tacgcacacg gcctgtccgg cgtctggatc ggcgtcggct
     1861 gcctcagcgg catcacgacc gcgtggctcg tgctggcgcg caagttcagg agcgaggccg
     1921 cgcgctaccg cgccatgacg atgcccgagt acttctccgc gaaattcccc gagaaagcca
     1981 acaccatccg ggtgctctcg acgctgatca tcgcgttctt cttcatcttc tacgtgggcg
     2041 cgcagttcgc cggagcgggg aagaccatcc aggagacgtt cggcctgcca gccatctggg
     2101 gccaggtgct gtgcgcggcc gtcatcatga cgtacgcgag tttcggcggg ttcatcagcg
     2161 tcgtggcggt ggacgccttc caggccgtcc tgatgatcct gacgctcatc gtcacgccga
     2221 tcgtcatgct cgcgaaggtg ctggcggggc cggtgtcgat cgggcaggcc ctcgaggcgg
     2281 cgggcccggg catgagctcg ctcacgggcg gggcggccgg cttcgccgcc gggatcctca
     2341 tcttcaacaa cttcgcatgg ttcttcggct acctcggggg gcagccgcag ctgaacgccc
     2401 gtttcatggg gatgcgggac gaccgtcagg tgcggatcgg ccggaacatc gccgtcgcct
     2461 ggacggtcgc ggcctatacc ggcgcggtga tgatcggcct ctgcgccctc gccctgttcg
     2521 ggccgaaggc catcggcgac gccgagctgg tgctgccgtt cgcgttgacc gcgctc
//
LOCUS       DAOSIF010000444         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_34992_length_2568_cov_3.014723, whole genome shotgun sequence.
ACCESSION   DAOSIF010000444 DAOSIF010000000
VERSION     DAOSIF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34992_length_2568_cov_3.014723"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1146
                     /locus_tag="PLT35_12300"
     CDS             82..1146
                     /locus_tag="PLT35_12300"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOG30052.1"
                     /translation="MNSEIVTLLGDKAGYLLEHSCTTVPKDLLHLPGPDFVDRVWAVS
                     DRNNRVLGNLQRIHATGRLAGTGYLSILPVDQGIEHSGGASFAKNPIYFDAENIVKLA
                     IEGGCNAVASTFGVLGAVSRKYAHKIPFIVKINHNELLTYPNKADQIMFGEIQQAADM
                     GAAAVGATIYYGSDQSGRQIVEVAKAFAEAHRLGMATVLWCYLRNSAFKTDKDYHVSA
                     DLTGQANHLGVTIEADIIKQKLPETNGGYKALNTGGSSYGKLDERIYTQLTTDHPIDL
                     TRYQVVNCYMGRAGLINSGGASGSNDFGEAVATAVINKRAGGMGLISGRKAFQRPMAE
                     GAKLLHTIQDVYLDAGITIA"
     gene            complement(1133..1390)
                     /locus_tag="PLT35_12305"
     CDS             complement(1133..1390)
                     /locus_tag="PLT35_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG30053.1"
                     /translation="MTPPVRCWSVYLLRCADGTFYAGSTTDVDARARAHDEGRGARYT
                     SGRTPVTVVYREACGSRSAALRRERALKRLTRLQKIALTRS"
     gene            complement(1387..2334)
                     /locus_tag="PLT35_12310"
     CDS             complement(1387..2334)
                     /locus_tag="PLT35_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30054.1"
                     /translation="MDMIDRYAHEVGQHLPHRLRADVEAELRSLLADAVEEKARQGGV
                     PAGRELQASVLRDFGRPQDVAARYAPGPQYLIGPRLYPAYLTAVKVMLPILAALIVAV
                     VAAGGFLAPGEEPSLTVFVRATGRFLWSALENLGVLTLVFALVERAMRQREPADQAFD
                     PAALPPVNDPDRVSYFGRIVALYLIALLAVAFNVFPGWVAVPVFHGSEGAAHPLLAPG
                     FGRYLPALNVWWGLAFLLNLAVLRRGRWTRPTRWLDFGLELGLAAILLRIVTGPPAFA
                     YDRPIKVVLGWILAFTAVSACVMLFRLLRRQPAEPWAAA"
     gene            complement(2352..>2568)
                     /locus_tag="PLT35_12315"
     CDS             complement(2352..>2568)
                     /locus_tag="PLT35_12315"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG30055.1"
                     /translation="LLSQKGLEIDEGTLYPLLRRLESQGLLRSAWRVEDGRPRRYYQA
                     SAQGAKLAKELTAEWRKLADALGTVIG"
BASE COUNT          440 a          906 c          879 g          343 t
ORIGIN      
        1 cggcctgggc cttcaccgct cttccgcggc cgcggccgcc tgagctgcgc cgcccccgcg
       61 ggttcgcgag gacatgccat catgaacagc gagatcgtca ccctgctcgg cgacaaggcc
      121 gggtacctgc ttgagcactc ctgcacgacg gtgccgaagg atctgctgca cctgcccggc
      181 ccggacttcg tggatcgcgt gtgggccgtg tccgaccgca acaaccgcgt gctcggcaac
      241 ctccagcgga tccacgcgac cgggcggctg gccggaacgg gctacctctc gattcttccg
      301 gtggaccagg gcatcgagca ctccggcggc gcgagcttcg cgaagaaccc gatctacttc
      361 gacgccgaga acatcgtgaa gctcgccatc gagggcgggt gcaacgcggt ggcctcgacc
      421 ttcggcgtcc tcggagcggt gtctcgcaag tacgcccaca agatcccgtt catcgtgaag
      481 atcaaccaca acgagctgct gacgtacccg aacaaggccg accagatcat gttcggcgag
      541 atccagcagg cggccgacat gggcgcggcg gcggtcgggg cgacgattta ctacggatcc
      601 gaccagagcg gccggcagat cgtcgaggtg gcgaaggcgt tcgccgaggc gcaccgcctc
      661 ggcatggcga cagtgctgtg gtgctacctg cgcaacagcg ccttcaagac cgacaaggac
      721 taccacgtga gcgcggatct caccggccag gccaaccatc tcggcgtgac aatcgaggcc
      781 gacatcatca agcagaagct gcccgagacc aacggcggct acaaggcgct caacaccggc
      841 gggtcgagct acggcaagct ggacgagcgc atctacacgc agctcacgac ggaccacccg
      901 atcgacctga cgcgctacca ggtggtgaac tgctacatgg gccgcgcggg gctcatcaac
      961 tcgggcgggg cctcgggcag caacgacttc ggcgaggccg tggcgacggc ggtcatcaac
     1021 aagcgcgccg gcggcatggg cctcatctcc gggcgcaagg cgttccagcg gccgatggcc
     1081 gagggcgcga agctcctgca cacgatccag gacgtgtacc tggacgcggg catcacgatc
     1141 gcgtgagggc gatcttctgc agccgggtca gccgcttcag ggcgcgctcg cgcctgagag
     1201 cggctgaccg cgatccgcac gcctccctgt acaccaccgt caccggcgtc cggccggacg
     1261 tgtagcgcgc gccccggcct tcgtcgtggg cccgcgcccg ggcgtcgacg tccgtcgtcg
     1321 agcccgcgta gaaggtgccg tccgcgcagc gcaggaggta gacggaccag cagcggaccg
     1381 gcggcgtcac gccgccgccc acggctcggc cggctgccgc cgcagcaggc ggaacagcat
     1441 cacgcatgcg ctgacggcgg tgaaggccag gatccacccc aacacgacct tgataggacg
     1501 atcgtaagcg aacgccggcg gtccggtgac gatgcgcagc aggatggcgg cgagccccag
     1561 ctcgagcccg aagtccagcc accgcgtcgg gcgcgtccat cggccccgtc gcagcaccgc
     1621 caggttgagg aggaacgcca ggccccacca cacgttgagc gccgggaggt agcggccgaa
     1681 ccccggcgcc agcagcggat gcgcggcccc ctcgctgccg tggaacacgg gtactgcgac
     1741 ccagcccggg aagacgttga aggccaccgc caaaagcgcg atcagataga gcgcgacgat
     1801 gcgcccgaag tacgacaccc ggtcggggtc gttcaccggc ggaagcgcgg ccggatcgaa
     1861 cgcctggtcg gcgggctccc gctgccgcat cgcacgctcg accagcgcga agacaagtgt
     1921 caggacgccg aggttctcga gcgcgctcca gagaaaccgg cccgtggccc ggacgaacac
     1981 cgtgaggctg ggctcttcgc cgggggccag aaaccctccc gcggcgacca ccgcgacaat
     2041 cagggccgcc aggatcggga gcatgacctt cacggccgtc aggtacgcgg ggtagagcct
     2101 cggcccgatc aggtactggg gccccggcgc gtaacgggcc gcgacgtcct gcggacggcc
     2161 gaagtcgcgg aggacgctcg cctgaagctc ccggccggcg gggacgccgc cttggcgcgc
     2221 cttctcttcg acggcatcgg cgagcagcga acgaagctcg gcttcgacgt ccgcccggag
     2281 gcggtgggga aggtgctgtc cgacctcgtg cgcgtagcga tcgatcatgt ccatttggct
     2341 tctctcctgt gtcagccgat caccgttccg agcgcgtccg cgagcttccg ccactcggcg
     2401 gtcagctcct tcgccagctt cgcgccctgc gcgctggcct ggtagtagcg ccgcgggcgg
     2461 ccgtcttcga cgcgccaggc gctcctcagc agtccctgcg actcgagccg gcgcaggagg
     2521 gggtacaggg tgccttcgtc gatctccagg cccttctggg agagcagt
//
LOCUS       DAOSIF010000445         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_35212_length_2554_cov_4.278912, whole genome shotgun sequence.
ACCESSION   DAOSIF010000445 DAOSIF010000000
VERSION     DAOSIF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35212_length_2554_cov_4.278912"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2554
                     /locus_tag="PLT35_12320"
     CDS             <1..>2554
                     /locus_tag="PLT35_12320"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG30056.1"
                     /translation="LGAERATAAELVARAIAGAARAGTPAADTARLRALAAGLAAASA
                     AAVPADPRGPEGDLAFWVRAVIDAIERLEEPASHPGERLHALAGRAARLADDMRFDFL
                     YDRRRRIFAIGYRLADAEGPGRLDASFYDLLASEARLASFVAIAKGDVPQHHWFHLGR
                     MVTNVKGRAVLVSWGGTMFEYLMPLLLMRGFRGTLLDRSCRASVWRQIEYGRQHGIPW
                     GISESAYSLTDRDGTYQYKAFGVPGLGLKRGLSDELVVSPYSTALASLLNAPAATANL
                     ELLRRVGLDGRFGFYEAVDYRPRTKVDPEAPDAPAKASPVIVRAYFAHHQGMSLVALA
                     NTVRDGVFVERFHADPRIRATELLLQERVPREAILSEPRPAKAAAAAAPVPVLASRRF
                     KSAHTVSPHAHALSNGRYTTMLTHAGGGWSAWHDLAVTRRREDRTSDFGAHFIYFRDP
                     WSGKVWSPTYLPTCVEPDSYEVTFAHDKATYRRRDDDLESQMQVAVSPEDDVEVRRVT
                     ITNRGDRPREVEITSYAEIVLGRPEDDLAHPAFGKLFIETEYDAQSAGLLFSRRPRAS
                     DDPAAWAFHVLGVDGRFGGAVEWETDRARFLGRGRNLARPAAIDGPLSGTTGAVLDPV
                     ASLRERVRLAPGAFVRLTFATGAAPARAAALTLVQKYRDASAASRVFSMAFTHAHITL
                     QHLGLTDSQAMLFDRLGSRVFGADATCISPTDLAGNTLGQPSLWAYGISGDLPIVLLR
                     VIDPAGVALARQLLHAQEYWRVKSLRADLVILNEQPVDYLDDLQGSLAALVREPRFAG
                     WHDKPGGVFLLRTDGMPEPDRRLLAAAARVVLRSDLGELEPQLDRPAPWLYPA"
BASE COUNT          327 a          991 c          886 g          350 t
ORIGIN      
        1 gttgggcgcc gagcgcgcca ccgcggccga gctggtggcc cgcgcgatcg ccggggctgc
       61 ccgcgccggg acgcccgccg ccgacactgc ccggctgcgc gcactcgccg ccggcctggc
      121 agccgcgtcg gccgcggccg tgccggccga cccgcgcggg ccggagggag acctggcctt
      181 ctgggtgcgc gccgtaatcg acgcgatcga gcgcctcgag gagcctgcct cgcaccccgg
      241 cgaacggctg cacgcgctgg ccgggcgggc cgcgcgtctt gccgacgaca tgcggttcga
      301 cttcctgtac gaccgccgga ggcgcatctt cgcgatcggc taccgcctgg ccgacgccga
      361 ggggcctgga cggctcgacg cgtcgttcta cgacctgctg gcctccgagg cacggctggc
      421 cagcttcgtc gccatcgcga agggcgacgt gcctcagcat cactggttcc acctcgggcg
      481 catggtgacc aacgtcaagg ggcgcgcggt gctcgtgtcg tggggcggca ccatgttcga
      541 atacctgatg ccgctgctgc tgatgcgcgg cttccggggg acgctgctcg atcgttcctg
      601 ccgcgccagc gtgtggaggc agatcgagta cgggcggcag cacggcatcc cctgggggat
      661 ctccgagtcg gcgtactcgc tcaccgaccg cgacgggacc tatcagtaca aggccttcgg
      721 cgtcccgggc ctcggcctca agcgcggcct ctccgacgag ctggtggtgt cgccgtactc
      781 caccgcgctc gccagcctgc tgaacgcccc ggccgccacc gcgaacctcg agctgctccg
      841 gcgggtcggc ctcgacggcc gctttggctt ctacgaggcg gtggactacc ggccgcggac
      901 gaaggtcgat cccgaagcgc cagacgcccc ggccaaggcg tcgccggtca tcgtccgggc
      961 gtacttcgcc caccaccagg gcatgtcgct tgtggcgctc gccaacaccg tccgcgacgg
     1021 cgtcttcgtc gagcggttcc acgccgaccc gcgcatccgg gccaccgagc tgctgctgca
     1081 ggagcgggtg ccgcgcgagg cgatcctctc cgagcctcgg ccggcgaagg ccgcggccgc
     1141 cgcggccccc gtcccggtgc tcgcgtcgcg ccgattcaag tcggcgcaca cggtgagccc
     1201 gcacgcgcac gccctgtcga acgggcgcta cacgacgatg ctgacgcacg cgggcggcgg
     1261 ctggagcgcg tggcacgacc tggccgtgac caggcgccgc gaggaccgca cctccgactt
     1321 cggcgcccac ttcatctact tccgcgaccc gtggtcgggg aaggtctggt cgcccaccta
     1381 cctgcccacc tgcgtggagc ccgacagcta cgaggtgacg ttcgcgcatg acaaggcgac
     1441 ctaccgccgg cgcgacgacg acctcgagag ccagatgcaa gtggccgtct cgcccgaaga
     1501 cgacgtcgag gtgaggcgcg tgacgatcac caaccgcggc gaccggccgc gcgaggtgga
     1561 gatcacgagc tacgccgaga tcgtgctcgg gcgccccgag gacgacctgg cccacccggc
     1621 gttcgggaag ctgttcatcg agaccgagta cgacgcgcag agcgcgggcc tgctcttcag
     1681 ccggcgcccc cgcgcctcgg acgacccggc cgcctgggcc ttccacgtgc tcggggtgga
     1741 cggccgcttc ggcggcgccg tggaatggga gaccgaccgc gcccggttcc tgggccgcgg
     1801 ccgcaacctt gcccggccgg ccgccatcga cggcccgctg tcgggaacca ccggggccgt
     1861 gctcgacccg gtcgcctcgc tgcgcgagcg cgtgcgcctc gctcccgggg ctttcgtccg
     1921 gctcaccttc gccaccggcg ccgcgccggc ccgcgccgcc gcgctgaccc tcgtccagaa
     1981 gtaccgcgac gcgagcgccg cctcgcgcgt cttctcgatg gcgttcacgc acgcgcacat
     2041 cacgctgcag cacctcggcc tgaccgacag ccaggcgatg ctcttcgacc gcctcgggtc
     2101 gcgcgtcttc ggcgccgacg ccacctgcat cagccccacc gacctcgcgg gcaacacgct
     2161 cggccagccc tcgctgtggg cctacggcat ctcgggcgac ctccccatcg tgctcctgcg
     2221 ggtcatcgac ccggcgggcg ttgcgctggc ccgccagctg ctgcacgcgc aggagtactg
     2281 gcgcgtcaag agcctgcgcg cagacctggt catcctcaac gagcagccgg tggactacct
     2341 cgacgacctg cagggatcgc tcgccgcgct ggtgcgcgag ccgcgcttcg cggggtggca
     2401 cgacaagccg ggaggcgtct ttctcctgcg gaccgacggg atgccggaac ccgaccgccg
     2461 cctgctggcc gccgcggccc gcgtggtcct ccgcagcgac ctcggagaac tggagccgca
     2521 gctggatcgc ccggcgccct ggctctaccc ggcg
//
LOCUS       DAOSIF010000446         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_35313_length_2548_cov_3.232651, whole genome shotgun sequence.
ACCESSION   DAOSIF010000446 DAOSIF010000000
VERSION     DAOSIF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35313_length_2548_cov_3.232651"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1130)
                     /locus_tag="PLT35_12325"
     CDS             complement(291..1130)
                     /locus_tag="PLT35_12325"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOG30057.1"
                     /translation="MSRWWNQRRFDAVELAFVSLLAALMALTAVRARHSRVSFVAYSA
                     SQEAARLEQLYGPSKYSRHEEEWIIRDYFGDRRGGVFVDVGANHYQHESNTYYLERTL
                     GWSGIAVDPQVDFAEGYRAHRPRTRFFAFFVSDFSDERVTLYLADGNSLVASSDKAFT
                     ERGGSRLLEPSLATRAVTVATIRLTDLLDRAGVSHIDLLSVDVELAEPRVLAGFDIDR
                     FAPELVCIEAHPEVRQAILDYFAAHRYVVVGRYLRADLQNLYFQPASSPGPPPMPPRA
                     GSR"
     gene            1423..1797
                     /locus_tag="PLT35_12330"
     CDS             1423..1797
                     /locus_tag="PLT35_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30058.1"
                     /translation="MGFIGIQRGAIAVHRQSAVTLKPRVHVARYAGKDGKLHVKRYSR
                     TLSALGRTQLQAAGGRLIQPTTYGHLMEFGTARGVAPRPFLRPAAFQQKSAFEAAMRA
                     MAPDVIEALRQHGAPGAFGRTA"
     gene            1794..2228
                     /locus_tag="PLT35_12335"
     CDS             1794..2228
                     /locus_tag="PLT35_12335"
                     /inference="COORDINATES: protein motif:HMM:NF022810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HOG30059.1"
                     /translation="MSVTTGKSALSAVHAAVYSVLKHDATLTTRAPGGVWDHVPQDPT
                     WPYVRLGGFVEDPDDTFGRQGRRVTFVVHAWSQYAGVSEGYGIIDRVLALLRYTTLTL
                     TGWEHHDTRHLTTEVDEPQLVDAVQVQHVWAEFEVFVAEVLP"
     gene            2225..>2479
                     /locus_tag="PLT35_12340"
     CDS             2225..>2479
                     /locus_tag="PLT35_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30060.1"
                     /translation="MTRYYEVIGAAPVVLDGRSVAPGVITTAELDAALEAFLLRLGAL
                     RIVDPATESELILPARHRPAQQTLAAPPDDRPAVAVQAAEE"
BASE COUNT          390 a          884 c          864 g          341 t
ORIGIN      
        1 ggccgcgcgg tgcagcaaaa cgacgcggcc ggccggccgc gggaacccgc tgaggcggcc
       61 gcccggcgcg ccgctccggg gccgccggcc aaatgcccga gccgcgtccc ggccggctcg
      121 gctgcttcgt ctgccgagcg cagcaggcgc tgatcggcgg cctctgccgc gcctgtggcg
      181 cagtcccgga tccgcattca gagagcggcc gccgcgggag cccgggtcgt ggccagcctc
      241 ggccagtggc ccgccatcgt cgccggccac ggtcgccggc cgccagcccg tcaccggctg
      301 ccggcacgcg gtggcattgg aggagggccc ggcgaagacg ccggctggaa gtagaggttc
      361 tgcaagtcgg cccgcaggta ccggcccacg accacgtagc ggtgcgcggc gaagtagtcg
      421 agaatcgcct gccgtacctc gggatgcgcc tcaatgcaga ccagctccgg cgcgaagcga
      481 tcaatgtcga atccggcaag aacccggggc tcggccaact cgacgtccac cgacaggaga
      541 tcgatgtgcg acacgccggc ccggtccagc aggtccgtca gccgaatcgt cgccaccgtg
      601 acggctcgcg tggccagcga cggctccagc aggcggctgc ctccacgttc ggtgaatgcc
      661 ttgtccgagg acgcgacaag ggagttcccg tccgcaaggt acagcgtgac gcgttcgtcg
      721 gagaagtcgg agacgaagaa cgcgaaaaag cgcgtgcgcg gcctgtgcgc gcgatagccc
      781 tccgcgaaat caacctgggg atcgaccgcg atacccgacc atcccagcgt gcgctcgagg
      841 tagtacgtat tgctttcatg ctggtagtgg ttggccccga cgtccacgaa gaccccgccc
      901 cggcgatcgc cgaagtagtc cctgatgatc cattcctcct catgacggga gtacttcgag
      961 ggcccgtaga gctgctccag gcgcgcggcc tcctgggaag cggaatacgc cacgaacgac
     1021 actctcgagt gacgcgcgcg gactgcggtc aaggccatca gagccgccag aagcgacacg
     1081 aacgcgagtt ccacggcgtc gaaccgcctc tggttccacc atcgcgacat gaggccctcc
     1141 acatgagcgc gaccgctcga gcggcacgat catcaggcgc agaaagccgg cgtcccgcca
     1201 gccgccctgc tccctgatcg aggctccgag atcctctgcg gggctcgcgg cgcgagctac
     1261 aggctgtcgg gattcgctcg cgagtgagcc tacgccttcg ccacgcagac tcttatcggc
     1321 accgtgccag cgagcctgtg caggctgaga gcggcagtcc cgggctacgg cggcggggcc
     1381 ttgcgccgcg ccatcgcggg ccgggccagt cagcagaccc tcatgggctt catcgggatc
     1441 cagcgtgggg caattgccgt gcaccggcag tcggcggtga cgctgaagcc gcgggtgcat
     1501 gtggcgcggt acgccggcaa ggatggcaag ctccacgtca agcggtatag ccgcaccctc
     1561 agcgcgctcg ggcgcacgca actccaggcg gccggcggcc ggctgatcca gccgaccacg
     1621 tacggccacc tgatggagtt cggcaccgcg cgcggcgtcg cgccgcggcc cttccttcgt
     1681 cccgcggcct tccagcagaa gtcagccttt gaggccgcca tgcgcgcgat ggctccggac
     1741 gtgatcgagg ccctccggca gcacggcgcc ccgggcgcgt tcgggaggac cgcatgagcg
     1801 tcaccaccgg caagtcggcg ttgagcgcgg tgcacgcggc ggtctacagc gtgctcaagc
     1861 acgacgccac gctgacgacg cgagctccgg ggggcgtctg ggaccacgtg ccccaggatc
     1921 cgacgtggcc gtacgtccga ttgggcggct tcgttgaaga cccggatgac acgttcggcc
     1981 gccaggggcg tcgcgtcacc tttgtggtgc acgcctggtc gcagtacgcc ggcgtctcgg
     2041 agggctacgg catcatcgac cgcgtgctgg cgctcctccg gtacacgacc ctcacgctca
     2101 cgggctggga gcatcacgac acgcgccacc tgacgaccga agtggacgag ccgcagctcg
     2161 tcgacgccgt gcaggtgcag cacgtctggg cggagttcga ggtctttgtg gcggaggtgc
     2221 tgccatgacg cggtactacg aagtgatcgg cgcggcgccc gtggtgctcg acggccgcag
     2281 cgtggccccc ggcgtcatca ccacggcgga gctcgacgcc gcgctcgagg ccttcctctt
     2341 gcgactcggc gccctccgga tcgtggaccc agccaccgag tccgagctca ttctgccggc
     2401 gcgccaccgg ccggcgcagc agacgctcgc cgcgccgccc gacgaccggc cggcggtggc
     2461 cgtccaggcg gccgaggag
//
LOCUS       DAOSIF010000447         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_35410_length_2543_cov_3.167605, whole genome shotgun sequence.
ACCESSION   DAOSIF010000447 DAOSIF010000000
VERSION     DAOSIF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35410_length_2543_cov_3.167605"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /locus_tag="PLT35_12345"
     CDS             <1..2197
                     /locus_tag="PLT35_12345"
                     /inference="COORDINATES: protein motif:HMM:NF018208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30061.1"
                     /translation="RDELRRWEKVLVARRQRVLAQAIAGADTRIAQARAAEAGAAARL
                     AEREAAVERVRLEAAGREQAAREARGTAHVHEVDIGRLEARMQADEARLASLAARAAD
                     LEREIAALAGRREPAAADRAAKAQAAARAGEERDDAAAALAAAQEVQAALHATMDGLE
                     GDVEAVRAEQFAAASAVTALQHAVQNALATRERVARDLARWTGEAGDLDIEEDRARRD
                     RQAASEALASAKATLEAVARGRAARDAELSALRAERDLAASALREREQELADASARLR
                     SLEEFEAARSAYEDGARLLLAAPGGPVAHAGSLADHLDVAPGYERAVEACLGDVLQYV
                     VVPSHDEAARALGIVKAHGAGRCGFVITGSRPGGPGAEAIDEAPPAPGLVPLASVASL
                     AGPHADALAPLVRRAWIADSYADAVAAAALTADPVATLDGEVCLGPRLVLGGGRRDGG
                     RGILQTKRDTRELRERLAADRSAVSALADTVAGLEHRLTLGAAALDAMRAEIHRVEMA
                     MVGSELRVAQADDELARIRQKRDLVGLDVRRAQEEAAALDAREAEARAAIARLAGDQR
                     AIDERLDAAQRTLFEAREGARQKGDGVRDAMARHARLVERATMAALEAARADEAAREI
                     ESRLAARQDERRQAGEDQEGLRQSVREAGLAFEAQLALVCELTRLSRRKGCQVLVATH
                     SPIVAALPGARILQLDEHGIHAVEWADLETVGHFRRFLAAPQQYLRHLD"
     gene            complement(2268..>2543)
                     /locus_tag="PLT35_12350"
     CDS             complement(2268..>2543)
                     /locus_tag="PLT35_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30062.1"
                     /translation="HGHRGRVYLDVSWEEVGLTVEIDGAHHAAGLADVDDALRQNEVT
                     IDQSMVLRIPVLGLRLAEGTFMAQVRRAYDEAARRRAAGIGAANLAS"
BASE COUNT          344 a          951 c          974 g          274 t
ORIGIN      
        1 gcgcgacgag ctgcggcggt gggagaaggt gctcgtcgcg cggcgccagc gcgtgctggc
       61 ccaggcgatt gcaggggccg acacgcgcat cgcgcaggcc cgcgccgcgg aggccggcgc
      121 ggccgcccgc ctcgccgagc gcgaggccgc cgtggagcgc gtgcgcctcg aggccgccgg
      181 gcgcgagcag gcggcgaggg aggcgcgcgg gaccgcgcac gttcacgaag tggacatcgg
      241 gcgcctcgaa gcgcgcatgc aggccgacga ggccaggctc gcctcgctcg ccgcccgcgc
      301 agcggacctc gagcgcgaga ttgcggcgct cgcggggcgg cgcgagcccg ccgccgcgga
      361 ccgggccgcg aaggcgcagg cggcggcgcg cgcgggcgag gagcgcgacg acgcggccgc
      421 ggcgctcgcc gcggcgcagg aggtgcaggc cgccctccac gccacgatgg acggcctcga
      481 gggcgacgtg gaggcggtcc gcgccgagca gttcgccgcg gccagcgccg tcacggccct
      541 ccagcacgcc gtccagaacg ccctcgccac gcgcgagcgc gtggccaggg acttggcgcg
      601 gtggaccggc gaagcgggcg acctcgacat cgaggaggac agggcgcgcc gcgaccggca
      661 ggccgcctcg gaagcgctcg cgtccgcgaa ggccacgctc gaggccgtcg cgcgcggccg
      721 cgccgcgcgc gacgccgagc tgtccgcgct ccgcgccgag cgcgaccttg ccgcgtcggc
      781 cctgcgcgaa cgggaacagg agctcgccga cgcatccgcc cggctgagat cgctcgagga
      841 attcgaggcc gcccggagcg cctacgaaga cggcgcacgg ctgctgctgg cggcgcccgg
      901 cggcccggtg gcccacgccg ggtccctggc agatcatctc gacgtggcgc cgggctacga
      961 gcgcgccgtc gaggcctgcc tgggcgacgt gctgcagtac gtggtggtcc cgagccacga
     1021 cgaggcggcc cgcgccctcg gcatcgtcaa ggcgcacggc gcggggcgat gcggcttcgt
     1081 gatcaccggc agccggcccg gcggcccggg cgcggaggca atcgacgagg cgcccccggc
     1141 gccgggcctg gttccgctcg cctccgtggc gtcgctcgca ggcccgcatg ccgacgcgct
     1201 ggccccgctc gtgcgccgag cctggatcgc cgactcgtac gccgacgccg tggccgccgc
     1261 cgccctcacc gccgaccccg ttgcgacgct ggatggagag gtgtgcctgg gcccgcggct
     1321 cgtcctcggc ggaggccggc gggacggcgg ccggggcatc ctgcagacca agcgcgacac
     1381 gcgcgaactg agagagcggc tggccgccga ccggtcggcg gtgtccgccc tcgccgacac
     1441 ggtggccggc ctcgagcatc ggctcacgct cggcgctgcc gccctggacg cgatgcgcgc
     1501 cgagatccac cgggtggaaa tggcgatggt cggctccgag ctgcgcgtcg cccaggcgga
     1561 cgacgagctc gcccgcatcc gccagaagcg ggacctcgtc gggctcgacg tgagacgggc
     1621 ccaggaagaa gccgccgcgc tggacgcgcg ggaagccgag gccagggccg ccatcgcgcg
     1681 cctggccggg gaccagcgcg cgattgacga gcggctggat gcggcgcagc gcacgctctt
     1741 cgaggcgcgg gaaggcgccc ggcagaaggg cgacggcgtc cgcgacgcca tggcccggca
     1801 cgcccggctc gtcgagcggg cgacgatggc ggccctcgaa gccgcaaggg ccgacgaggc
     1861 ggcgcgcgag atcgagagcc ggctggccgc gcggcaggac gaacggcggc aggccggcga
     1921 ggaccaggaa ggcctgcggc agtcggtgcg ggaggccggg ctggcgttcg aagcccagct
     1981 cgcactcgtc tgtgagctca cccggttatc gcggcgcaag ggctgtcagg tgcttgtcgc
     2041 gacccactcc ccgatcgtcg cagcgctgcc tggcgcgcgc atcctgcaac tggacgagca
     2101 cggcatacat gctgtggagt gggccgatct ggagaccgtt ggccactttc ggcgcttcct
     2161 ggccgccccg cagcaatacc tgcgccacct cgactgaggc accccagtca gatccaccca
     2221 cccctccagg ccggccctct cccagttttg gtgtgtcgca ttctgcgcta ggacgccaga
     2281 ttcgccgcac caataccggc ggcccgacgg cgggccgcct cgtcgtaggc gcgccgcacc
     2341 tgggccatga acgtgccctc ggccagccgg agaccgagga cgggaatccg tagcaccatg
     2401 gactggtcaa tcgtcacctc gttctgccgg agtgcgtcat cgacgtccgc gaggcctgcc
     2461 gcgtggtgcg ccccgtcgat ctccacggtg agacccacct cctcccatga gacatcgagg
     2521 taaacgcgcc cccgatgccc atg
//
LOCUS       DAOSIF010000448         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_35446_length_2541_cov_3.730893, whole genome shotgun sequence.
ACCESSION   DAOSIF010000448 DAOSIF010000000
VERSION     DAOSIF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35446_length_2541_cov_3.730893"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /gene="maeA"
                     /locus_tag="PLT35_12355"
     CDS             <1..1312
                     /gene="maeA"
                     /locus_tag="PLT35_12355"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HOG30063.1"
                     /translation="ADIDQIFGELPSPSVNLIVVTDGERILGLGDLGSDGMGIPVGKV
                     TLYVAAGGLHPACCLPMTLDVGTNNQRLLDDPLYLGWKHPRLDGQQYWDFIERFVLGV
                     KRNFPEALIQWEDFAKHKAFTVLERYQDRVLSFDDDIQGTGAVAAAVVMTAARIKRRP
                     ASEERFLIAGMGQAGTGIAVNIQALLEREGLSEREARARIFALDLPGLLCEDMPGLSK
                     WQQPFARARADVAGWALDASDRIGLLDVVRNAKPTVLVGVTAQPGLFGQDVLSAVAAY
                     EPQPVVMALSNPTSKSECRPEDVARLTDGRGLIATGSPFGDVEWEGRTLAVSQCNNLY
                     VFPGVGLGALVAKAAKVTRGMFMAASQALSDMVTPEQAARGQLLPPIEDIRAVSRAVA
                     KAVAVEARDAGLGRLIDDDQYERAIARAQWEPAYAPYRPGRARG"
     gene            1414..1857
                     /gene="mscL"
                     /locus_tag="PLT35_12360"
     CDS             1414..1857
                     /gene="mscL"
                     /locus_tag="PLT35_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOG30064.1"
                     /translation="MGRTSMMKEFKEFVMRGNVMDLAVGVIIGAAFGKIVGSLVNDVL
                     MPVIGLLLGRVDFSSLFVTLGEGRFATPAAAKEAGVATLNYGVFINTIIEFLIVAFAI
                     FMVVKWVNALRRPAPAAAPTTKDCPHCATAIPVAARKCPNCTSPL"
     gene            complement(1951..>2541)
                     /locus_tag="PLT35_12365"
     CDS             complement(1951..>2541)
                     /locus_tag="PLT35_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOG30065.1"
                     /translation="RSTSCSRPSPGGSADATGSGDGVARYKAAYFRILAQEIRRPAPP
                     DPSTPAWRRFKGVYPGVRELLDALSRRQDVFLALLTGNYEQGARIKLEHFGLSHYFSC
                     GAFGDDSVSRPALVPVAMARARAAGCPAVAPADVVIIGDTPLDVACAIDAGVRCVAVA
                     TGGYDRDALRQAGARTVFGTFSDTDAVVACLTEPGA"
BASE COUNT          378 a          871 c          923 g          369 t
ORIGIN      
        1 cgccgacatc gaccagatct tcggcgagct cccctctccg agcgtgaacc tcatcgtcgt
       61 caccgacggc gagcggattc tcggcctggg cgacctcggg tcggacggca tgggcatccc
      121 ggtgggcaag gtcacgctct acgtcgccgc ggggggcctg caccccgcgt gctgcctgcc
      181 catgacgctc gacgtcggaa cgaacaacca gcgcctcctc gacgatccgc tgtatctcgg
      241 gtggaagcat ccgcggctgg acggccagca gtattgggac ttcatcgagc ggttcgttct
      301 cggcgtgaag cgcaacttcc cggaagcgct cattcagtgg gaggacttcg cgaagcacaa
      361 ggccttcacc gtgctcgagc ggtaccagga ccgggtgctg tcgttcgacg acgacatcca
      421 gggcaccggt gccgtggcgg ccgcggtcgt catgacggcg gcccgcatca agcgacgtcc
      481 ggcctccgag gagcgcttcc tgatcgccgg catgggccag gccggcacgg gcatcgccgt
      541 gaacatccag gcgctgctcg agcgcgaggg cctgagcgag cgcgaggccc gcgcgcggat
      601 cttcgccctg gatctgccag gcctgctgtg cgaggacatg cccgggctgt cgaagtggca
      661 gcagcccttt gcgcgggcgc gcgccgacgt ggccggctgg gcgctcgatg ccagcgatcg
      721 catcggcctc ctcgacgtcg tgcgcaacgc gaagcccacg gtgctcgtcg gcgtcaccgc
      781 ccagcccggc ctgttcggcc aggacgtgct gtccgccgtc gcggcgtacg agccgcagcc
      841 ggtggtgatg gcgctctcga atccgaccag caagagcgag tgccggccgg aggacgtggc
      901 caggctcacg gacgggcgcg gcctcatcgc caccggcagc cccttcggcg acgtcgagtg
      961 ggagggccgc accctggcgg tgtcgcagtg caacaacctg tacgtgttcc cgggcgttgg
     1021 cctgggggcg ctcgtggcca aggccgcgaa ggtgacgcgc ggcatgttca tggcggccag
     1081 ccaggctctc agcgacatgg tgaccccgga gcaggcggcg cgagggcagc tgttgccgcc
     1141 gatcgaggac atccgcgccg tgtcgcgcgc ggtggccaag gccgtggccg tcgaggcgcg
     1201 cgacgcgggg ctcggccgct tgatcgacga cgatcagtac gagcgggcca tcgcccgggc
     1261 ccagtgggaa ccggcatacg cgccgtaccg gccggggagg gcgagaggct aggagaaccg
     1321 gcgatcggaa ccccgagtcg ggcgatccgg cccggccgcc cgccgcggca ctcggccgct
     1381 cggggccgga actcgagcgc gttgcagcac gtgatgggga gaacgtcgat gatgaaggaa
     1441 ttcaaggaat tcgtgatgcg cgggaacgtg atggacctgg ccgtcggggt catcatcggc
     1501 gccgccttcg ggaagatcgt cggctcgctc gtgaacgacg tgctgatgcc cgtcatcggc
     1561 ctgctgctcg ggcgcgtgga cttctcgagc ctgttcgtca ccttaggtga gggccggttc
     1621 gccaccccgg ctgccgcgaa ggaagcggga gtggccacgc tgaactacgg cgtgttcatc
     1681 aacacgatca tcgagttcct gatcgtcgcc ttcgcgatct tcatggtggt caagtgggtc
     1741 aacgcgcttc ggcgcccggc gccggcggcc gcgccgacga cgaaggactg cccgcactgc
     1801 gcgacggcca tccccgtcgc cgccaggaag tgcccgaact gcacctcgcc gttgtaggcg
     1861 gctgacggcc tgagcccggg tcgtcgtcgt ggggtcagat ccgcatatat gcggatctga
     1921 ccccgtttcc ggcggtggag cccggggtct tcaggccccc ggttccgtca agcacgcgac
     1981 caccgcgtcc gtgtcggaaa aggtgccgaa gaccgttcgg gcccccgcct gacgcagcgc
     2041 gtccctgtcg tagccgccgg ttgcgaccgc gacgcagcgg acccccgcgt cgatcgcgca
     2101 cgccacgtcc agcggcgtgt cgccgatgat gacgacgtcg gccggggcca cggcgggaca
     2161 gccagccgcg cgcgccctgg ccatggcgac gggcacgagc gcgggccggc tcacgctgtc
     2221 gtcgccgaag gccccgcacg agaagtagtg cgagaggccg aagtgctcga gcttgatgcg
     2281 cgccccctgc tcgtagttcc ccgtcaggag cgcgaggaag acgtcctgcc gccgagacag
     2341 ggcgtcgagc agttcgcgca cgcccgggta tacgcccttg aaccggcgcc acgccggcgt
     2401 cgatggatcc ggcggcgccg ggcggcggat ctcctgggcg aggatccgga agtacgcggc
     2461 cttgtagcgc gccacgccgt cgccggaccc ggtggcgtcg gccgatccac caggcgatgg
     2521 ccgggagcac gaggtagaac g
//
LOCUS       DAOSIF010000449         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_35593_length_2533_cov_3.412429, whole genome shotgun sequence.
ACCESSION   DAOSIF010000449 DAOSIF010000000
VERSION     DAOSIF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35593_length_2533_cov_3.412429"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLT35_12370"
     CDS             <1..1062
                     /locus_tag="PLT35_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG30066.1"
                     /translation="RAFCPFSQIDIVRNTTPADHESRVYTFRIIEYEEDGRNLIVSRR
                     VLQEEEQQARAAEIRQSIVPGEVLTGRVASVRDFGAFVDLGGGIQGLLHVSEMGWSRV
                     ANAAQVVGPGQEVTVKVLRVEADGQKIALGLKQLSPDPWATVPDRYDVGQLRPGRVTR
                     VTDFGAFVELEPGVEALAHVSTFEPTGRAGGWAATVPPGTTGVFEILSLDFERKRIGV
                     ALAMAGLVPEGGTVPREREIAAGARLVGKVERHEAFGVFVFLAPGRTGLMPMSETGLE
                     KEADVVKAFPVGSDVEVEVLEVDPAGRRIRLSRQAVLDAQGRDELRDYTERTEAAGPQ
                     AFGGTLADKLRGALEPRRK"
     gene            1606..>2533
                     /locus_tag="PLT35_12375"
     CDS             1606..>2533
                     /locus_tag="PLT35_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOG30067.1"
                     /translation="MDEPSPPCSAAEGSLSSTCKYALFIAILGLCINTTEASGAAPSS
                     LTTREPAIKAVELRWDRLAPYRAHGTFRFAFGHGGQAEILRRIESVRARWTDDRGDTV
                     FGFQAVYPEPYERFLDFVDWKLIEMPIPRLQRLGRGDEERFLYEYNWPAPVRRSVVTA
                     YQEKAPETFAHSLALRPGVADELVRPNGLLRLISCREWAVRVAKLSALPEAGFEHFLF
                     DVDRASPSVVRKLLRDWQDDCCSDCGGRFIGGVDVAISFTRPRHGDNGLDNLVPAHGK
                     CNNSKGDFLASAEHVERWTELSGGLDADLAAVA"
BASE COUNT          388 a          845 c          916 g          384 t
ORIGIN      
        1 cgcgccttct gccccttctc ccagatcgac atcgtccgga acacaacacc ggccgatcac
       61 gagagccgcg tctacacctt ccgcatcatt gagtacgagg aagacggccg gaacctcatc
      121 gtgtcgaggc gagtgctgca ggaagaggag cagcaggcgc gcgcggccga gatccggcaa
      181 tcgatcgtgc ccggcgaggt gctgaccggg cgcgtcgcgt cggtgcgcga tttcggcgcg
      241 ttcgtcgatc tcggcggcgg cattcagggg ctcctgcacg tttcggagat gggctggtcc
      301 cgcgtggcga acgccgcgca ggtggtcggg ccgggccagg aggtcaccgt caaggtgctg
      361 cgcgtcgagg ccgacgggca gaagatcgcg ctcgggctga agcagctgag ccccgacccg
      421 tgggccaccg tgccggaccg gtacgacgtg ggccagctca gaccaggacg ggtgacgcgc
      481 gtcaccgact tcggcgcgtt cgtcgagctt gagccaggcg tggaggcgct ggcgcacgtc
      541 tccacgttcg aaccgacggg ccgggccggc ggctgggcgg ccaccgtccc gccgggcacg
      601 acgggggtgt tcgagatcct gagcctcgac ttcgagagga agcggatcgg cgtggcgctc
      661 gcaatggcag gcctggtgcc ggagggcggg acggtcccac gggagcgcga gatcgccgcg
      721 ggcgcgcgcc tcgtcgggaa ggtggagcgg cacgaggcct tcggggtgtt cgtgttcctc
      781 gcgccgggcc gcaccggcct catgcccatg agcgagaccg gcctcgagaa ggaagccgac
      841 gtggtgaagg ccttccctgt cgggagcgac gtcgaggtcg aggtgctcga ggtggacccg
      901 gcgggacggc gcatccggct cagtcgccag gccgtcctcg atgcgcaggg tcgcgacgag
      961 ctgcgcgact acacggagcg cacggaagcc gccgggccgc aggcgttcgg cggaacgctg
     1021 gccgacaagc tccgcggggc gctcgaaccg cgcaggaagt gaggggcggc acgaggcgac
     1081 gaacgcggac tcccgtacgc ccgggtggat ccggcgcgcc tccacggaat cctcgagagg
     1141 cagatggagg atctgcgggc gatggcgcgc gtggtcgccg ggctgccggg aagtcgagcc
     1201 ccgtcctcgc cgtgctccgc tagcgcctca gtcagcacgc ctcgggcgtc caccgccgcc
     1261 gcacccgcac cgcgtcgatg gcgaatcgcg cgccgggaac gttcgcgcca tcgagaccgc
     1321 ctcgttcagc gaaccggcga tacgccgcct gcgtgtcttc gcgaacatgg cgcgggatat
     1381 gcgagcgcgt tcttgagccc gcgcccgtcc gccgatctct tcgctttcgc gtccgaaacg
     1441 cagttctggc ggaagctgta gcggagcgtg cagctccgcg gcattgccct ggccaccctc
     1501 tccctcgcat tctcggtggc ggcgatggcg aggaggacgc gcgggcgggc ggcaaccagt
     1561 gatggggcgg cggacgtcgc tgctacggtg ttaccatctc cgccgatgga cgagcctagc
     1621 ccgccctgca gcgctgcgga agggagcctc tcgtcaacct gcaagtacgc cctgttcata
     1681 gcgatcctgg gtctctgcat caacacaacc gaggccagcg gcgccgcccc atcgtcgctg
     1741 acgacgcgtg agccggcaat caaggccgtg gagttgcgtt gggaccgcct cgcgccgtac
     1801 cgcgcgcacg gcacgttccg gttcgccttc ggacacggcg gacaggcgga gatcctgcgg
     1861 cgaatcgaga gcgtgcgcgc gcggtggacc gatgatcgcg gcgataccgt attcggcttc
     1921 caggccgtct accccgagcc gtacgagcgg ttcctcgact tcgtcgactg gaagctcatc
     1981 gagatgccga ttccccggct gcagagactc ggcaggggcg acgaggaaag gttcctctac
     2041 gagtacaact ggccggcgcc ggtgcggagg tcggttgtca ctgcctacca ggagaaagcg
     2101 cctgagacgt tcgcccactc gctggcgctg aggcctggcg tcgccgatga gctcgtccgg
     2161 ccgaacggac tcctacgcct gatctcctgt cgcgaatggg cggtcagggt cgcgaagctg
     2221 agcgctcttc ctgaggccgg cttcgagcac ttccttttcg atgtcgaccg cgcgtcgccg
     2281 agcgtagtcc ggaagctgct gcgcgactgg caggatgact gctgctccga ctgcggcggc
     2341 cgcttcatcg gcggagtcga cgtggccatt tcattcactc gtccccggca tggcgacaac
     2401 ggcctggaca acctcgtgcc agcgcatggc aagtgcaaca acagcaaggg cgatttcctc
     2461 gcgtcggccg agcacgtgga gcgctggacg gagctcagcg gcgggcttga cgcggacctg
     2521 gccgccgtcg ctc
//
LOCUS       DAOSIF010000450         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_35886_length_2517_cov_3.793664, whole genome shotgun sequence.
ACCESSION   DAOSIF010000450 DAOSIF010000000
VERSION     DAOSIF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35886_length_2517_cov_3.793664"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PLT35_12380"
     CDS             complement(<1..1332)
                     /locus_tag="PLT35_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOG30068.1"
                     /translation="MRYPSAPPALALLAGCAIGSVRHFDCGPAAAAILAAWTACLCCW
                     HRGAARGFVASALALCFLCGASLSSSAAHRATHTPLRAALAASREPGPVRDALSEPVV
                     LSGTLSADASPLERGARLVVDVATVRVDGRAAGAPGRVSLTVTGSPPPSAIGTWRRGR
                     RVEVTAQLRRPARYLDAGVPDQELALARRGTTLVGVVKSALLVRVTGEGSVLAEAASE
                     ARARVRRCLDETVGGYDARSAGIVRAILLGDRAGLDDQTEIRLQEAGTYHVLAISGGN
                     VAILASVLLGLGRLAGLRPRVSNLAVAGALAAYAGLVGSGASVVRATQMAVIYLCAHA
                     ADHRTRPLNAAAASAGASAVLDPLLLFDVGAWLTYGATIAILVGTPRLLPSLASSWRW
                     ARAPAGILAASCAAEAALFPVAAMVFHRVTAAGLLLNFAAIPLMTVVQVGGM"
     gene            complement(1450..>2517)
                     /locus_tag="PLT35_12385"
     CDS             complement(1450..>2517)
                     /locus_tag="PLT35_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG30069.1"
                     /translation="IVYDPGALDHYMTTAVDASPEHPVLIDRFLEDAFELDVDAVGDS
                     TGALVIAGIMEHIEEAGIHSGDSSCVVPPFMVAERHLEIIRKHTRRIAQALGVVGLMN
                     AQFAIKDGVVYVLEVNPRASRTVPYLSKATGVPLARVAARAMAGRTLAELGLTGDLAV
                     NGVFVKSPVFPFVRFPGVDTILGPEMKSTGEVMGAAASFGEAFAKAQTAVGQELPTAG
                     TVFISVNNSDKPNVLAVARDLAALGFTLVATRGTAAYLRSHGVEAGTVFKVNEGRPNV
                     ADLVVNGSIQLIVNTPLGRASFFDDRSVRRRAMLHGVPCITTLTGAAAAVSGIRAIRG
                     REVTVRALQDYHAESATLPPP"
BASE COUNT          396 a          920 c          897 g          304 t
ORIGIN      
        1 catgccgccg acctgcacca ccgtcatcag cgggatcgcc gcgaagttca gcaggagccc
       61 cgccgccgtc accctgtgaa acaccatcgc ggcgaccggg aacagcgcgg cctcggcggc
      121 gcacgaggcc gcgaggatcc ccgcgggcgc cctggcccat cgccacgatg aggccagcga
      181 tggcagcagc cgcggcgtgc ctacgagaat cgcgattgtc gccccatacg tcagccaggc
      241 cccgacgtcg aagagcagca ggggatccag gaccgcgctc gcgccggcag aagcggcggc
      301 ggcattgaga ggccgggtcc gatgatcggc ggcgtgggcg cacaggtaga tcaccgccat
      361 ctgggtggcc cgcaccaccg atgcgccgct cccgacgagg ccggcgtacg cggccagagc
      421 cccggccacg gcgaggttcg agacgcgggg gcgcagtccc gcgagccggc cgagaccgag
      481 caggacgctt gcaagaatcg cgacgttgcc gccggagatc gccagcacgt ggtaggtgcc
      541 ggcctcctgc aaccggatct cggtctggtc gtccaggccc gcgcggtcgc cgagcagaat
      601 cgcccgcacg atgcccgccg agcgtgcgtc gtagccgccg acggtctcgt cgaggcaccg
      661 gcgcacgcgg gcccgcgcct cgctggcggc ttcggcaaga acgcttccct ctccggtcac
      721 ccgcacgagc agcgcgctct tcacgacgcc gacgagcgtg gtgccgcggc gagccagcgc
      781 gagttcctgg tccggcacgc cggcgtcgag gtacctcgcc ggccggcgca gctgcgcggt
      841 gacctcgacc cgccggcccc tgcgccacgt cccgatcgcc gacggcggcg gcgagcccgt
      901 gacggtcagg ctgacacggc ccggtgcgcc ggccgcacgg ccatccacgc ggaccgtcgc
      961 gacatcgacc acgagccggg cgccacgctc cagcggcgac gcgtcggctg acagggttcc
     1021 cgacagcacg acgggttcgg agagagcatc tcggaccgga ccgggttccc ggctcgccgc
     1081 cagcgcggcc cgcagtggag tgtgcgtggc ccgatgggcc gctgacgatg agagagatgc
     1141 gccgcaaagg aagcacagcg ccagggcgga cgccacgaac ccgcgggcgg cgccgcggtg
     1201 ccagcagcac aggcaggcgg tccaggctgc caggatcgcc gcggcggccg ggccgcagtc
     1261 aaagtgccgg actgatccaa tcgcgcagcc cgccaggagc gcgagggcgg gcggcgccga
     1321 cgggtaacgc atgcgtgccg gtcacgcaac gagcgttcca gcggggaccg gcgctcccag
     1381 gctcgcatcc gcgaaggaaa ggccggcgcc agtgcgggcc cccggagagg gatcgaagat
     1441 cctgcacgtc tacgggggcg gcagcgtggc ggattccgcg tggtagtcct ggagcgcgcg
     1501 caccgtcacc tcgcgcccgc ggatggcccg aatgccgctg accgccgccg cggcgccggt
     1561 gagcgtcgtg atgcagggca cgccgtgcag catcgcgcgc cgccgcaccg agcggtcgtc
     1621 gaagaacgac gcccgcccga ggggggtgtt cacgatcagc tggatcgacc cgttcacgac
     1681 gaggtccgca acgttgggtc ggccttcgtt caccttgaag accgtccctg cctcgacgcc
     1741 gtgtgaccgg aggtaggccg ccgtcccccg cgtcgcgacc agggtgaagc ccagcgccgc
     1801 gagatcccgg gccacggcga gcacgttcgg cttgtcgctg ttgttcaccg agatgaacac
     1861 cgtgcccgcc gtcggcagct cctgccccac cgccgtttgc gccttggcga acgcctcgcc
     1921 gaacgaggcg gcggccccca tcacctcgcc ggtggacttc atctcgggac cgaggatggt
     1981 gtcgacgcct gggaacctga cgaacggaaa gaccggcgac ttgacgaaca cgccgttgac
     2041 ggcaaggtcg cccgtgagcc cgagctcggc gagcgtccgc cccgccatcg ccctggcggc
     2101 cacgcgcgcc agcggcacgc cggtcgcctt cgacaggtac ggcacggtcc gcgaggccct
     2161 gggattcacc tcaagcacgt acacgacgcc gtccttgatg gcgaactggg cgttcatgag
     2221 cccgacgacg ccgagggcct gcgcaatgcg ccgcgtgtgc ttccggatga tctcgaggtg
     2281 acgctcggcc accatgaacg gcggcacgac gcacgaactg tcccccgagt gaatgccggc
     2341 ctcctcgatg tgctccatga tgcccgcgat gacgagcgcc cccgtcgagt cgcccaccgc
     2401 gtccacgtcg agctcgaagg cgtcctcgag gaaccggtcg atgagcacgg ggtgttccgg
     2461 cgacgcatcg acggccgtcg tcatgtagtg gtcgagggcc ccagggtcgt agacgat
//
LOCUS       DAOSIF010000451         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_36099_length_2505_cov_3.762449, whole genome shotgun sequence.
ACCESSION   DAOSIF010000451 DAOSIF010000000
VERSION     DAOSIF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36099_length_2505_cov_3.762449"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLT35_12390"
     CDS             complement(<1..180)
                     /locus_tag="PLT35_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30070.1"
                     /translation="MIRPGDLLTLVPEKAAAGGRMLARHDGMVILVAGAIPGERVTAR
                     ADRVESGGRLVQDQQL"
     gene            complement(190..960)
                     /locus_tag="PLT35_12395"
     CDS             complement(190..960)
                     /locus_tag="PLT35_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30071.1"
                     /translation="MDIDQYCREIEAYLCRRNRGHLVRLVGPAFDLARGWASAGIPLN
                     VVYQGIDRRLERASARGSARRPMRIEFCEADVLDAFDAWRRAVGVHGVSGAAEETAGE
                     AGDGGLGDGAAAARRGGRTEPGPARRSRESLSTHVDRVIVRLTALRTGGAAGEWDAVL
                     DGLVRRLDALHPAARRARGEARERILADLAALDAEMLERARACAPPGVVADVEREAEA
                     DLEPFAARLAPAALAAARRRVADRALRERLRLPVLTLD"
     gene            complement(969..1748)
                     /locus_tag="PLT35_12400"
     CDS             complement(969..1748)
                     /locus_tag="PLT35_12400"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOG30072.1"
                     /translation="MLLAIDVGNTNIVIGVFEGEALGRSWRLATACERTGDELGLMFV
                     DLLERAGVRIPAIDGVVMASVVPQLTGPMADMANRYLGLTPLIVEADTDIGLANLYAH
                     PSEVGADRLVNAVAAWAAFGRERGQPLIVVDFGTATTFDAISARGEYLGGVICPGIQI
                     SADALFQRAAMLPRIDVQKPSTVIGRTTVASMQAGLFFGYVEMAQGLVRRIRAELGGA
                     AFCVATGGLAEVIAPETDVIDAVDGDLTLRGLKMVWDRRTR"
     gene            complement(1758..>2505)
                     /locus_tag="PLT35_12405"
     CDS             complement(1758..>2505)
                     /locus_tag="PLT35_12405"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG30073.1"
                     /translation="RSIGSTNDLAGRLADGGAGEGTTVAAETQTSGRGRLGRAWYSPE
                     GAGLYVSVVIRPDRETAGPDAPPPGVPPHGVPSALTLAAGVALAEAVREATGLAAELK
                     WPNDLVVRRRKLAGILAEASSRGGAVEHVILGFGVNVKPAAYPADLADRVTSLEAELG
                     HPVDRGALLALALAKIGEGRETLRSGGLSRILARWRELAPSSVGARVEWRRADGPVEG
                     RTAGLDGDGALLVEARGRVERVVAGEVIWR"
BASE COUNT          327 a          994 c          869 g          315 t
ORIGIN      
        1 gagctgctga tcctggacga gccgacctcc ggactcgacc cggtcggcgc gcgccgtgac
       61 gcgctcgccc gggatggccc ccgccacgag gatcaccatc ccgtcgtggc gtgcgagcat
      121 gcgaccgccg gcggccgctt tctcggggac cagcgtcagg agatctccag ggcggatcac
      181 ggcgagcgcc tagtcgagcg tcagcaccgg gagccgcagg cgctcccgca aggcgcggtc
      241 cgccacgcgg cggcgggccg cggccagcgc ggccggcgcc agcctcgctg cgaacggctc
      301 caggtcggcc tcggcctcgc gctcgacgtc ggccacgacg ccgggcgggg cgcatgcgcg
      361 cgcgcgttcg agcatctcgg cgtcgagggc cgccaggtcg gccaggatcc gctcgcgcgc
      421 ctcgccgcgg gcgcgccgag ctgccgggtg cagggcgtcg agccgccgca cgagcccgtc
      481 gagcacggcg tcccactcgc cggccgcgcc tcccgttcgg agcgccgtca gcctgacgat
      541 cacgcggtcc acgtgcgtcg agagggattc gcggctccgg cgcgccggcc ccggctcggt
      601 cctgccgccg cgcctggcgg ccgccgcgcc gtcgcccagc ccgccgtcgc cggcctcccc
      661 ggccgtctcc tctgccgccc cggacacgcc gtggacaccg acggcgcgcc gccacgcgtc
      721 gaacgcgtcc aggacatcgg cctcgcagaa ctcgattcgc atcggccgcc tggcgctgcc
      781 cctggcggac gcgcgctcga ggcggcggtc gatgccctgg tacacgacgt tcagcggaat
      841 gccggcggac gcccagccgc gcgccaggtc gaaggccggg ccgaccagcc gcacgagatg
      901 cccccggttg cgcctgcaca ggtacgcctc gatttcgcgg cagtactggt cgatgtccat
      961 ctcccccgct atctcgtccg ccgatcccag accatcttca ggcccctgag cgtcaggtcc
     1021 ccgtccacgg cgtcgatgac gtccgtctcc ggcgcgatca cctcagcgag gcctcccgtc
     1081 gcaacgcaga aggccgcgcc gccaagctcc gctcggatgc gccgcacgag cccctgggcc
     1141 atctcgacgt acccgaagaa caagcccgcc tgcatcgagg cgaccgttgt ccggccgatg
     1201 acggtcgagg gcttctgcac gtcgatgcgc ggcagcatcg ctgcgcgctg gaacagggcg
     1261 tccgccgaga tctggatgcc cgggcagatc acgccgccca ggtattcgcc ccgcgcggag
     1321 atggcgtcga acgtggtggc cgtgccgaag tccacgacga tgagcggctg cccccgctcg
     1381 cggccgaacg cggcccaggc cgccacggcg ttgaccagcc ggtccgcgcc cacttcggat
     1441 gggtgcgcgt agaggttcgc gagcccgatg tcggtgtcgg cctcgacaat gagcggcgtg
     1501 agccccaggt accggttcgc catgtcggcc atcggccccg tgagttgcgg aacgactgag
     1561 gccatcacca cgccgtcgat ggcggggatc ctgacgcccg cgcgctccag caggtcgacg
     1621 aacatcaggc cgagttcgtc ccccgtccgt tcgcaggcgg tcgccaggcg ccagcttcgg
     1681 ccgagcgcct cgccctcgaa cacgccaatc acgatgttgg tgtttccgac gtcgatggcc
     1741 agcagcatgt tcgtgtccta ccgccagatg acctcgcctg cgaccacgcg ctccacgcgg
     1801 ccgcgcgctt ccacgagcag cgcgccgtcg ccgtccagcc ccgcggtgcg gccctccacc
     1861 ggtccgtccg cacgccgcca ctcgacgcgg gccccgactg acgagggcgc cagctcccgc
     1921 cagcgggcga ggatgcggga cagcccgccg gaacgcagcg tctcgcgccc ctcgccgatc
     1981 ttcgccagcg ccaatgccag gagggccccg cgatcgaccg gatggccgag ttccgcctcg
     2041 agggaggtga ccctgtcggc gagatccgcg ggatacgcgg ccggcttcac gttcacgccg
     2101 aacccgagga tgacgtgctc gacggcgccg ccgcgcgacg aggcctcggc gaggatgccc
     2161 gcgagcttcc gccggcggac cacgagatcg ttcggccact tcagctccgc cgcgagccct
     2221 gttgcctcgc gcacggcctc ggccagcgcc acgcccgccg ccagcgtcag cgccgacggc
     2281 actccgtgcg gcggcacgcc cggcggcggc gcatccggcc ccgcggtctc gcggtcgggc
     2341 cggatcacga cggacacgta caggccggcg ccctcgggcg agtaccacgc acggccgagg
     2401 cgtccgcgac cggaggtctg cgtctcggcg gccaccgtgg tgccctcgcc ggcgccgccg
     2461 tcggcaagcc ggccggcgag gtcgttcgtc gagccaatcg agcgg
//
LOCUS       DAOSIF010000452         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_36187_length_2501_cov_4.111202, whole genome shotgun sequence.
ACCESSION   DAOSIF010000452 DAOSIF010000000
VERSION     DAOSIF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36187_length_2501_cov_4.111202"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLT35_12410"
     CDS             complement(<1..319)
                     /locus_tag="PLT35_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOG30074.1"
                     /translation="MAPPRSRAVLAVPRWFTQRPIQPMGLLYLASMLRNAGHEVRVVD
                     GEVDALDPSHVADIVASERPEFFGITSTTAQATRAFALAEEVKRRTPGTFVTLTAHST
                     ALGT"
     gene            complement(579..1760)
                     /locus_tag="PLT35_12415"
     CDS             complement(579..1760)
                     /locus_tag="PLT35_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30075.1"
                     /translation="MQHKFLLFGIAAVAALAIACGDEVTTPAAPSSTSPPTVDAASTD
                     AASDGSTLKATAPAPQLPANGSTLDSLTPNLVIANSTLKFLGDVSLVSTLQYQFVVET
                     SEGVPVRNVLTGAGAALTGSRVPADVLQPDTTYRWRARAEYKGTYGPWSAYWTFVTPK
                     SAAALPSFQNATELWDNLTDGRTIGTLAGGANLVAGKGVYLPTLDSHVTYTLQSTLTS
                     GTIDFAVEGLKSDSKGEKTKVVSMQEGSSEFTTNPFRFNVEKRGKDHPDSGKLRMRII
                     TGDAVNKVFDSDRILPPKPLVASMVYVFHVSWGGGLVRLVVREGSTTGPLLVDNSFSY
                     QGTYRPNPHVVHIGGPIPRGGAIDATVPGITVRYFYVSQGRQWPAFNLATVLGLGGVG
                     R"
     gene            1920..>2501
                     /locus_tag="PLT35_12420"
     CDS             1920..>2501
                     /locus_tag="PLT35_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30076.1"
                     /translation="MAPGSARRRARRSPALAVPLLSLLLTAPQPEAQAQPGERQAPAA
                     PPRLAVAHLARAVAPGEAVLLTVTAPGPIEALDVRAFGGRFMGYPGASPATWHALVGI
                     DLDTRPGRARVEIEARTPDGRVLQAAHSLAVARKDFPTRRLSVAPGFVTPPPSERARI
                     ERERVRTAQILAEVTPAPLWGEGFVRPAPGAVIS"
BASE COUNT          354 a          867 c          928 g          352 t
ORIGIN      
        1 gcgtgccgag cgcggtcgag tgcgcggtga gggtgacgaa ggtgccgggc gtgcggcgct
       61 tgacctcctc ggcgagcgcg aaggcccgcg tcgcctgcgc cgtcgtcgag gtgatgccga
      121 agaactcggg gcgctcggag gccacgatgt ccgcgacgtg cgacggatcg agcgcgtcaa
      181 cctcgccatc gacgacccgg acctcatggc ccgcgttcct cagcatcgag gcgaggtaca
      241 ggaggcccat cggctggatg ggacgctggg tgaaccagcg gggcacggcg agcacggcgc
      301 gcgagcgggg aggggccata cgcagactcg gagaaggccg ccagcggccc gcgcggcgta
      361 ctggcagccg cgggatgccg ggcggtcgtg ttcctgacga tgatacctcg gagggcggtt
      421 gagggaaaac acagcgagcg ccccgccgtg cgccgcggcc gggcggccgt catcccctgc
      481 ggcgaggcgg cgcgcgacaa ccgcagtacg cgagcagggc cgggcgccgg atgacctccg
      541 gcggcccggc ccctcgtttc ggcacgcggc ggcgaatgct aacgcccgac tccgccgagc
      601 cccagcaccg tcgccaggtt gaacgccggc cactgccttc cctgcgacac gtagaagtag
      661 cggacggtga tgccgggcac ggtggcgtcg atcgcgcccc cgcgcgggat cggcccgccg
      721 atgtgcacga cgtgcgggtt cggccggtag gtgccctgat acgaaaagga gttgtccacc
      781 agcagggggc cggtcgtact gccctcccgc acgacgagcc tgaccagccc gccgccccac
      841 gacacgtgga acacgtacac catcgacgcg accagcggct tgggcggcag gatgcgatcg
      901 gaatcgaaga ccttgttcac ggcgtcgccg gtgatgatgc gcatccggag ctttccgctg
      961 tccgggtggt ctttgccgcg cttctcgacg ttgaagcgga acgggttggt ggtgaattcg
     1021 ctgctcccct cctgcatgct gacgaccttc gtcttctcgc ccttcgagtc ggacttgagg
     1081 ccctcgaccg cgaagtcgat cgtgccggac gtcagcgtgc tctgcagcgt gtaggtcacg
     1141 tgcgagtcga gcgtcggcag gtagacgccc ttgccggcca ccagattggc cccgcccgcc
     1201 agcgtgccga tggtcctgcc gtccgtcagg ttgtcccaca gctccgtggc attctggaag
     1261 gacggcagcg ctgccgccga cttcggcgtg acgaacgtcc agtatgccga ccaggggccg
     1321 taggtgccct tgtactcggc cctggcgcgc caccggtacg tggtgtccgg ctggagcacg
     1381 tcggcgggca cacgcgaccc tgtcagcgcc gccccggcgc ccgtcagcac gttgcgcacg
     1441 ggcacgccct cgctcgtctc gacgacgaac tggtactgga gcgtgctcac gagcgacaca
     1501 tcgcccagga acttcagcgt ggagttggcg atgacgaggt tcggcgtcag cgagtcgagg
     1561 gtggagccgt tggcaggaag ctgcggcgcc ggggccgtgg ccttcaaggt cgacccgtcg
     1621 gaggccgcat cggtcgacgc ggcgtcgacc gtcggaggcg aggtggaact cggcgcggcg
     1681 ggtgtcgtga cctcatcgcc gcacgcgatg gccagcgcgg ccacggcggc gatgccgaag
     1741 agcaggaact tgtgctgcat gcggacactc cgaagctgga agagaacggc gcgggccgct
     1801 tgcccgaccc tcgcatgata ctccagaccc ctgcctcttc gaaacaccac gcgggcccgg
     1861 gcgcccgccg cgagggtgag gcctggggcg gtggcgcggg cccggtgtat ggtgtacgga
     1921 tggcacctgg tagtgcacgc cgccgcgcgc gtcgcagccc cgctctcgcg gttccgctcc
     1981 tgtcgctcct gctcaccgcc ccgcagccgg aggcgcaagc ccagcctggc gagcggcagg
     2041 cgccggcggc cccgccgcga ctcgcggtcg cgcacctggc cagggcggtc gcgccgggcg
     2101 aagcggtgct cctcaccgtc acggcgcctg ggccgatcga ggcactcgac gtgcgggcgt
     2161 tcgggggccg gttcatgggc tacccgggag cctcgccggc aacgtggcac gcgctcgtcg
     2221 gcatcgacct ggacacgcgg ccgggccggg cccgcgtgga gatcgaggcc cggacaccgg
     2281 acgggcgggt gctccaggcg gcgcattcgc tggccgtcgc acgcaaggac ttccccacgc
     2341 gccgattgtc ggtggcccca gggttcgtca cgccgccgcc atccgaacgg gcgcgcatcg
     2401 agcgggagcg cgtccgcacg gcgcagatcc tggctgaggt cacgccggcg ccgctgtggg
     2461 gagaagggtt cgtgcggccc gctcccgggg cggtgatctc c
//
LOCUS       DAOSIF010000453         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_36247_length_2498_cov_3.059353, whole genome shotgun sequence.
ACCESSION   DAOSIF010000453 DAOSIF010000000
VERSION     DAOSIF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36247_length_2498_cov_3.059353"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLT35_12425"
     CDS             <1..530
                     /locus_tag="PLT35_12425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG30077.1"
                     /translation="IGGLLAAYRHLVDRDPGTPRLVLAGRVTPAAGAWVAQAQAPPLA
                     GRVDVLGYVADERRSALYHGARVLVLPSFEEGFGLPALEAMALGVPVVASTSGALPEV
                     VGSAGLLVEATDTRALADAIRAVVSHAALWAELRQNGLARSSAFKWPEAAAALLLAYG
                     GWLDRARKNAPAGRT"
     gene            535..1656
                     /locus_tag="PLT35_12430"
     CDS             535..1656
                     /locus_tag="PLT35_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG30078.1"
                     /translation="MRIGIDARELTGRTTGVGRYLAELLREWAAPASAVAGRHQFVLY
                     SARTLGEELEPAMAGLGAEARVLPGSGGTRWEQLSLAAAANRDGLDVFFAPGYTAPVR
                     ARCPIALTIHDLSYFAHPEWFRWREGVRRRELTRQSANRASVVLTDSEFSRGEIVRHL
                     RLPDSRVRVVPLGAGAPAWFAAAADGEADAAVPPEPCVLYVGSIFNRRRVPDLVAAFP
                     AVAAAVPGARLDIVGENRTFPFQDIDALSRKTGLGDRIRVRPWVPEAELANAFRRASA
                     FVFLSEYEGFGLTPLEALACGVPVVLLDTPVAREVCGPAALYVAPNDLSGLASAIVTL
                     LGDAGARRVLMAEASGVLARYSWARTARETLLAIESAGA"
     gene            1661..2491
                     /locus_tag="PLT35_12435"
     CDS             1661..2491
                     /locus_tag="PLT35_12435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG30079.1"
                     /translation="MPRVTIVIVTFNTKAEVGACLRSLAVHPPEAPHDVIVVDNASTD
                     GTAEAIRAGWPKIRLIEPGRNLGFAAGNNLGIRSAAGEFVLLLNSDTIVPAGAVDALV
                     GGLAARPDCAAAGPRIVDAEGRPELSFGRMIGPFNEARQKAIVRLQLRGFGPALRYVD
                     RITRRAGHPDWVSGACLLVRRTEALAAGLLDERFFLYAEDVDFCAALRARGGRILFEP
                     AAEIVHLRGASRRTRPVETERAYRRSQLAFYEKHQPRWVPCLRAYLRMRGRLPPDLPS
                     "
BASE COUNT          311 a          890 c          925 g          372 t
ORIGIN      
        1 acatcggcgg cctcctggca gcgtaccggc acctggtgga tcgcgatccg ggcacgccgc
       61 ggctcgtgct ggcgggccgc gtgactcctg cggcgggcgc atgggtggcg caggcccagg
      121 cccccccgct ggcgggccgc gtcgacgtgc tcggctacgt cgccgacgag cggcggagcg
      181 cgctgtacca cggtgcccgg gtgctggtgc tgccgtcctt cgaagaggga ttcggcctgc
      241 cggcgctgga ggccatggcg ctcggcgtgc cggtggtggc gtccacgtcg ggagcgctcc
      301 cggaggtcgt cgggagtgcg gggctgctcg tggaggcaac cgacacgcgc gccctggccg
      361 acgctatccg ggcggtggtg tcgcacgccg ccctgtgggc cgagttgcgc cagaacggcc
      421 tggcccggtc ctcggcgttc aagtggccgg aggctgcggc cgcattgctg ctcgcctacg
      481 gcggctggct cgaccgcgcc cggaagaacg cccctgccgg aaggacatga cgcgatgcga
      541 atcggcatcg atgccaggga actgacgggc cggacgaccg gcgtcgggag atatctcgcg
      601 gaactgctcc gcgagtgggc ggcgcccgcc agcgcggtcg ccgggcgcca ccagttcgtc
      661 ctctactcgg cccggacgct cggcgaggag ctcgagcctg cgatggccgg cttgggggcc
      721 gaagccaggg tcttgccggg gtcgggaggc acccggtggg agcaattgag cctggcggcc
      781 gccgcgaacc gggacggcct ggacgtgttc ttcgcaccgg gctacacggc gccggtcagg
      841 gcccgctgcc caattgccct gacgatccac gacctctcgt acttcgcgca ccccgagtgg
      901 ttccgctggc gcgaaggcgt gcgccgccgg gaactgacac ggcagtcggc caaccgagca
      961 tcggtcgtgc tgaccgactc tgagttttcg aggggcgaaa tcgtccgcca cctgcgcctg
     1021 cccgattcac gggtccgcgt ggtgccgctc ggcgcgggtg cgccggcctg gttcgcggcg
     1081 gccgcggacg gcgaggccga cgccgcggta ccgccggaac cctgcgtgct ctacgtcggc
     1141 tcgatcttca accgacgccg cgtgccagat ctcgtcgccg cctttcccgc cgtggcggcc
     1201 gctgttccgg gagcgaggct ggacatcgtc ggcgagaacc gcacgtttcc cttccaggac
     1261 atcgacgccc tgagccggaa gaccggcttg ggcgatcgca tccgggtgcg gccctgggtg
     1321 cccgaggctg aactggcgaa cgcattcagg cgcgcatcgg ctttcgtgtt cctgtccgag
     1381 tacgagggat ttggcctgac gccgctggag gcgctcgcct gcggcgtgcc cgtggtgctc
     1441 ctcgacacac cggtcgcgcg cgaggtctgc gggcccgcgg cgctgtatgt cgcgccgaac
     1501 gacctctccg ggctcgcgtc ggccatcgtc acgctccttg gcgacgcggg cgcccgccgc
     1561 gtgctgatgg ccgaggcctc tggcgtgctc gcccgctact cctgggcgag gaccgcgcgc
     1621 gagaccctgc tggccatcga atcggccggg gcctgacggc gtgccgcgcg tcaccatcgt
     1681 cattgtcacg ttcaacacga aggccgaagt cggcgcgtgc ctccgctcgc tcgccgttca
     1741 cccgcctgaa gccccgcacg acgtgatcgt ggtcgacaat gcctccaccg acggcactgc
     1801 ggaggcgatc cgggcggggt ggccgaagat ccgcctgatc gagccggggc ggaacctggg
     1861 cttcgccgcc ggcaacaacc tcggcattcg gagcgcggcg ggcgagttcg tcctgttgct
     1921 gaacagcgac accatcgtgc cagccggcgc ggtggatgcg ctcgtcggcg gcctggctgc
     1981 gcggcctgac tgcgcggcgg ccggcccgcg catcgtcgac gcggaggggc ggccggagct
     2041 gtcgttcggc cgcatgatcg gaccgttcaa cgaggcgcgg cagaaggcga tcgtcaggct
     2101 gcagctgcgc ggcttcggcc cggccctgcg gtacgtcgac cggatcacgc gccgcgccgg
     2161 gcatcccgac tgggtcagcg gggcctgcct gctcgtccgc aggacggagg cgctggccgc
     2221 gggcctgctg gacgagcggt tcttcctcta cgcggaagac gtggatttct gcgcggcgct
     2281 ccgggcgagg ggcggccgca tcctgttcga accagccgcc gaaatcgtcc acctgcgggg
     2341 cgcgtcgcgc cggacgcgcc ccgtcgaaac cgagcgcgcc taccgcagga gccagctcgc
     2401 cttctacgag aagcaccagc cgcgatgggt gccgtgcctg agggcctacc tccgcatgcg
     2461 cggacgcctg ccccccgatc tgccaagctg agccgagc
//
LOCUS       DAOSIF010000454         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_36423_length_2488_cov_5.042335, whole genome shotgun sequence.
ACCESSION   DAOSIF010000454 DAOSIF010000000
VERSION     DAOSIF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36423_length_2488_cov_5.042335"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PLT35_12440"
     CDS             <1..1187
                     /locus_tag="PLT35_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30080.1"
                     /translation="ASGATLALIHPVYYLVRTGAPSRLAGASDIRLVTAPEFFAALAD
                     PNLGLLPNAPMFTAAVVLASCALLVRAVRTRSPRALCSPGVCAAAVSAAWLAFVAATA
                     TNVNHGGTPGLSRYATWFIPLAIPLFQQADRAFPRAGRSMAAIALAAAALSAWAYRPS
                     RPEAAASPTRLASFLWSRHPSLYDPLPEVFAERLRGLDENWLPVATPGCEKVLLPGRG
                     GRDSVWPMPCAPVPVPARCQAPGALCYANRTRTGYAFAAARVSAPQAFRLDRGAAWPP
                     SAEAAAAGILASLAWWEMRIAPPGSAGAIVAGARRAGRVFAWQAEDRLFVFALDTAPG
                     ASLSLEPAREMRGGFTAPDGAVAAPPVELRPGNGPQDLALPAPQPWVILVASARSGRD
                     GG"
     gene            complement(1167..1379)
                     /locus_tag="PLT35_12445"
                     /pseudo
     CDS             complement(1167..1379)
                     /locus_tag="PLT35_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(1395..1820)
                     /locus_tag="PLT35_12450"
     CDS             complement(1395..1820)
                     /locus_tag="PLT35_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30081.1"
                     /translation="MAILSTADTARVREMLGAMVDPVRLVFFTQTLNCETCEPTGRIL
                     KELAELSDLITVEEHNLLLERDEAAKLGIDRVPAIAVLGAQDYGIRFLGIPSGYEFMS
                     LLDAVLTVSKADSGLGEASRALLAGLPSPLELQVFVTPT"
     gene            1984..>2488
                     /locus_tag="PLT35_12455"
     CDS             1984..>2488
                     /locus_tag="PLT35_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30082.1"
                     /translation="MAQPVTGRAAWAAAALLLAQAAPGGAQAPAGAANPIGIFLRCTG
                     QEDPRKALEAVKSLGLDTIQVSRLPDRYYTPEGAREFSALLRETGMRADSVVAVFAGE
                     SYKDRETVVRTVGFRPLHLIPERLAYARSCVDFAAAIGARLVTFHMGFLPEDPDNPAY
                     RAMLDAVT"
BASE COUNT          279 a          963 c          903 g          343 t
ORIGIN      
        1 ttgcatccgg cgcgaccctc gcgctcatcc acccggtcta ctacctcgtg cggacgggcg
       61 cgccgtcgcg gctggcgggc gcgtcggaca tccggctggt gacggcgccc gagttcttcg
      121 cggcgctcgc cgaccccaac ctcggcctgc tgcctaacgc cccgatgttc acggcggcgg
      181 tagtcctggc ctcgtgcgcc ctgctcgtcc gcgcggtccg cacccgctcg ccgcgggcct
      241 tgtgctctcc cggcgtctgc gccgccgccg tctcggcggc gtggctggcc tttgtcgccg
      301 ccacggccac gaacgtgaac cacggcggca cgccgggcct cagccggtac gcgacgtggt
      361 tcatcccgct ggccatcccg ctgttccagc aggccgaccg cgcgttcccg cgcgcggggc
      421 gctccatggc cgcgatcgcc cttgccgccg ccgcgctcag cgcgtgggcg taccgcccgt
      481 cgcgcccgga ggcggcggcc tcgcccaccc ggctcgcgtc gttcctgtgg tcgcgccatc
      541 cgtcgctgta cgatccgctg cccgaggtct tcgccgagcg cctgcgcggc ctcgacgaga
      601 actggctgcc ggttgcgacg cccggatgcg agaaggtgct gctgccgggc cggggagggc
      661 gcgacagcgt gtggccgatg ccgtgcgcgc cggtccccgt cccggcgcgc tgccaggcgc
      721 cgggcgcgct ctgctacgca aaccgcacgc gcacgggcta cgccttcgcg gccgcgcgcg
      781 tctcggcgcc gcaggccttc cgcctcgatc gcggcgccgc ctggcccccg tccgccgagg
      841 ccgccgccgc cggcatcctg gcgtcgctcg cgtggtggga gatgcgcatc gcgccgcccg
      901 gcagcgccgg cgccatcgtc gccggcgctc ggcgcgcggg ccgggtcttc gcgtggcagg
      961 cggaggacag gctgttcgtg ttcgcgctcg acacggcgcc cggcgcctcc ctgagcctcg
     1021 agccggcgcg ggagatgcgc ggcggcttca ccgcgcccga tggggccgtg gccgcgccgc
     1081 ccgtcgagct gcgcccgggc aacggcccgc aggacctcgc cctgcccgcg cctcagccgt
     1141 gggtcatcct cgtcgccagc gcccgctcag ggcgcgacgg cgggtagcgc gccgacggcc
     1201 gtgcgcacga agtcctcttc cggccgcgat cccaggatct cgacgctgcc gttgaccacg
     1261 gtcttcggca cgccgctcac gcggtactgg cgcacgagat cggggtactc gcccgcctcg
     1321 acgatgatcg aggtgacgcg cgggctctcg accgccatcc ggttggccag gctgaccgcc
     1381 tggggacagt gcggtcaggt gggcgtgacg aacacctgca gctccagggg cgacggcagg
     1441 cccgcgagca gcgcgcggct ggcctccccg agcccggagt cggccttcga cacggtcagc
     1501 accgcgtcga gcagcgacat gaactcgtag ccggacggga tgccgagaaa gcggatgccg
     1561 taatcctgcg cgcccagcac ggcgatggcg ggcacgcgat cgatgccgag cttcgccgcc
     1621 tcgtcgcgct cgagcagcag gttgtgctcc tcgacggtga tgagatcgct cagctccgcc
     1681 agctccttca ggatccggcc cgtcggctcg caggtctcgc agttcagggt ttgcgtgaag
     1741 aaaaccaggc gcacggggtc gaccatggcg ccaagcatct cgcgcacgcg cgcggtgtcg
     1801 gccgtggaga ggatcgccat ccccccatta tggcaggttc gcgggcggcg cgcggccccg
     1861 ggccggcccg cgccagcccg tccggtggac atccccgcgc ccagccgcta cgatcggccc
     1921 gacgcggcgc cccggcgggc cgatcccggc cggcggcttc gcgcaatcga cgggaggtgg
     1981 caggtggcac agccggtgac gggcagggcc gcgtgggcgg cggcggcgct cctgctcgcg
     2041 caggcggcgc ccgggggcgc gcaggcgccg gccggggcgg ccaacccgat cggcatcttc
     2101 ttgcgctgca ccggccagga ggacccgcgg aaggccctcg aggcggtgaa gtcgctcggg
     2161 ctggacacga tccaggtgag caggctcccc gaccgctact acacgcccga gggcgcgcgc
     2221 gagttctcgg ccctgcttcg cgagaccggc atgcgcgccg actcggtcgt cgccgtgttc
     2281 gcgggcgaga gctacaagga ccgcgagacc gtcgtccgca cggtcggttt ccggccgctg
     2341 cacctgatcc ccgaacggct ggcctacgcc cgctcgtgcg tggatttcgc cgcggcgatc
     2401 ggcgcgcgcc tggtgacgtt ccacatgggc ttcctgccgg aggacccgga caacccggcc
     2461 taccgcgcga tgctggatgc cgtcaccg
//
LOCUS       DAOSIF010000455         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_36424_length_2488_cov_4.277435, whole genome shotgun sequence.
ACCESSION   DAOSIF010000455 DAOSIF010000000
VERSION     DAOSIF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36424_length_2488_cov_4.277435"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLT35_12460"
     CDS             <1..605
                     /locus_tag="PLT35_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30083.1"
                     /translation="VALLALAGATLGLGGAWPGGAFGRGCVTAVTAVGAAGLALAIAG
                     ACALLLRRIQDPALRAYSSPGDFFNLGFFIVALSALGLGWATRQDGTPGALAIAAGLL
                     RWDLAVPIPALLGAGIVLSALLLAYIPLTHMSHFVGKYFTYHAVRWDDAPLGEARRLR
                     TALAASLAHRPTWAASHMRGRGDGTWADVVATNPSREDGP"
     gene            602..2200
                     /locus_tag="PLT35_12465"
     CDS             602..2200
                     /locus_tag="PLT35_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG30084.1"
                     /translation="MRPRTKFSDLARADREPVARIDPADLPPLSSPGPPPAKLAAAWP
                     ARMDAAGYPLDGTRVLAFPDPATKAERDALVGAFLSGLEKLFSRENNWTFLQPLLLSM
                     EHCARCQTCSDACHVFESSGREELYRPTFRSEIFRRLYFEHVKGGGLVSAWQHGRVEV
                     TWPLLARLGELAYRCNLCRRCAQACPIGVDNGLIAHEIRKLFSQELGIAPTELHASGS
                     MLQMKVGSSTGMNAAAVRDNASFIDEEMTEKTGVAVETPWDVEGADVLLIHNAGEILS
                     WPENPGAFAVILNAAGVSWTMSSDIAGYDSVNYGVWYDDAQLARVARMHAEIAKALKV
                     KKIVVGECGHAHKALLVVADRLLDGDLRIPRESALTLLRDIVAGGRLRLDPSRNDFPV
                     TLHDPCNVVRLMGVVEPQREVLRLVCPRFREMTPHGVDNYCCGGGSGFAVMGGNGFQD
                     WRSQVAGRRKLQQVLDAFSDCLEPAVRKYVCAPCSNCKGQIRDLLAFHDVWRRHGIMY
                     GGLAELVVNAMKDVNPGFIEWEWR"
     gene            2235..>2488
                     /locus_tag="PLT35_12470"
     CDS             2235..>2488
                     /locus_tag="PLT35_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30085.1"
                     /translation="MDTRYADAFERFLSTFPGYAATSALDALRASDYARLDALGHVYL
                     DYTGGGLFSSSQIRRHQDLLLGSVLGNPHSGNPTSRASSGL"
BASE COUNT          334 a          890 c          878 g          386 t
ORIGIN      
        1 ccgtcgccct gctcgccctg gcgggtgcca cgctcggctt gggcggcgcg tggcccggcg
       61 gcgcgttcgg ccgcggctgc gtgacggcgg tcacggccgt cggcgccgca ggcctcgccc
      121 tggcgattgc cggcgcgtgc gccctgctcc tgcggcggat ccaggatccc gccctgcgcg
      181 cgtactcgag cccgggtgac ttcttcaacc ttggcttctt catcgtggcc ttgtcggccc
      241 tgggccttgg ctgggcgacg cgccaggacg gaacgcccgg ggcgctcgcg atcgcggcgg
      301 gcctgctgag gtgggacctg gccgttccca tcccggccct ccttggggcc ggcatcgtcc
      361 tgtcggccct gctgctggcg tacatccctc tgacgcacat gtcgcacttc gtcgggaagt
      421 acttcacgta tcacgccgtc cgatgggacg acgcgccgct tggcgaggcg cggcggctcc
      481 gcaccgcgct tgcggcgagc ctggcgcacc ggccgacgtg ggccgcctcc cacatgcgcg
      541 ggcgcggcga tggcacctgg gcggacgtgg tcgcgacgaa cccttcgcgc gaggacgggc
      601 catgaggccg cgcacgaagt tctcggacct ggcgcgggcc gatcgcgagc ccgtcgcacg
      661 gatcgacccg gccgacctcc ccccgctgtc gagcccgggg ccgccgccgg cgaagctcgc
      721 ggccgcgtgg cccgccagga tggacgccgc cgggtatccg ctcgacggga cgcgcgtcct
      781 ggcgtttccc gatcccgcga cgaaggccga aagggacgcc cttgtcggcg cgttcctgtc
      841 ggggctggag aagctcttct cgcgcgagaa caactggacg ttcctgcagc cgctcctgct
      901 cagcatggag cactgcgcgc gctgccagac ctgctcggac gcctgccacg tcttcgagtc
      961 gtccggacgg gaggagctgt accggccgac gttccgatca gagatcttcc gcaggctgta
     1021 cttcgagcac gtcaagggcg gcggcctcgt ctcggcctgg cagcacgggc gcgtcgaggt
     1081 gacctggccg ctcctggcgc gcctcggcga gttggcctac cgctgcaacc tctgccgccg
     1141 gtgcgcgcag gcgtgcccga tcggtgtgga caacgggctc atcgcccacg agatccgcaa
     1201 gctcttcagc caggagctgg gcatcgcgcc gacggagctc catgcctcgg gctcgatgct
     1261 ccagatgaaa gtggggtcgt ccaccggaat gaacgcggcg gccgtcaggg acaacgcctc
     1321 gttcatcgac gaggagatga cggagaagac cggcgtcgcg gtggagacgc cgtgggacgt
     1381 ggagggcgcg gacgtcctgc tgatccacaa cgcgggcgag atcctctcct ggcccgagaa
     1441 ccccggcgcg ttcgccgtga tcctgaacgc cgccggggtc tcctggacga tgtcgtcgga
     1501 catcgcgggg tacgactcgg tgaactacgg cgtctggtac gacgacgcgc agctggcccg
     1561 cgtggcgcgg atgcacgcgg agatcgcgaa ggcgctgaaa gtgaagaaga tcgtcgtcgg
     1621 cgagtgcggc cacgcccaca aggccctgct cgtcgtcgcc gaccggctgc tcgacggcga
     1681 tctccgcatt ccccgcgaga gcgcgctcac cctgctgcgg gacatcgtgg ccggcggccg
     1741 tttgcgcctc gacccgtccc gcaacgactt cccggtgacg ctgcacgacc cgtgcaacgt
     1801 cgtccgcctg atgggcgtcg tcgagccgca gcgcgaggtg ctgcggctcg tgtgcccgcg
     1861 gttccgcgag atgacgccgc acggcgtcga caactactgc tgcggcggcg gcagcggctt
     1921 cgcggtcatg ggcggaaacg gattccagga ctggcgatcg caggtcgccg ggcgcaggaa
     1981 gctgcagcag gtcctcgacg ccttctcgga ctgcctcgag cctgccgtcc ggaagtacgt
     2041 gtgcgcgccc tgcagcaact gcaagggcca gatccgggac ctgctggcct tccacgacgt
     2101 gtggcgccgc catggcatca tgtacggcgg gctggcggag ctcgtcgtca acgcgatgaa
     2161 ggacgtcaac cctggcttta tcgagtggga gtggcgctga cgcggtgacg cgggcgccgc
     2221 ggcgatcagc atccatggac accaggtacg cggacgcatt cgagcggttc ctgagcacgt
     2281 ttcccggcta cgcggccacc tccgcgctgg acgccctgcg cgcgtccgac tacgcgcgcc
     2341 tggacgcgct cgggcacgtg tacctcgact acaccggcgg cggcctcttc tcgtcatcgc
     2401 agatccggcg gcaccaggat ctcctcctcg gcagcgtgct cggcaacccg cactccggga
     2461 acccgacatc ccgcgcctcg tccgggct
//
LOCUS       DAOSIF010000456         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_36685_length_2475_cov_2.980579, whole genome shotgun sequence.
ACCESSION   DAOSIF010000456 DAOSIF010000000
VERSION     DAOSIF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36685_length_2475_cov_2.980579"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1329)
                     /locus_tag="PLT35_12475"
     CDS             complement(163..1329)
                     /locus_tag="PLT35_12475"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG30086.1"
                     /translation="MTTRTRVAIILITAPVLVFALVGGLMGKGRAAGETYPHLRVFDD
                     VFSLTTDNYVERVDVDRLMHGAMRGLAESLDPDSAYLTAADARLLESGADAGPAEVGL
                     DLTRQYYLRVISARDQSPAARAGLSPGDFIRAIDRQPTREMSVWEGMRRLRGAPGSTV
                     SLLVIRGNAAEPHEMTLTREALVPLAPASRIIDGAVGYVRVPDITAGTPASLREACRR
                     LQQAGAKALAIDLRGTSRGPLEAGLAMARLFVRAGTLAILDGRGTSRQVVAAGEADGE
                     VTLPIAVLTDFGTAGAAELFTAALSGNKRAETLGERTAGRAARQRLFPLPDGAALWMS
                     HAWYLTPAGAAIHERGLQPDVPVAQPDVEFGASAPAGDATLDEAVKRLRAKMAA"
     gene            complement(1326..>2475)
                     /locus_tag="PLT35_12480"
     CDS             complement(1326..>2475)
                     /locus_tag="PLT35_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG30087.1"
                     /translation="AGLLLCGALLAPGGAAAQAPGRAQADALDRRAAARIQSLRREAE
                     SLSSRGRTLVEELRKLEVERDLRAAEADLRTRELEAVQADLDRTTARALELEQSARSQ
                     LPDLSARLVELYKIGNGGYLRLLLNVDDLREMGRAYRFVSGLQGADRARVAEHRRTLA
                     ELRKAQSAIEARREEAARTQAEVARARDAAAAAVAAHEGLIRSIDARRDLAERFVREL
                     EEARQALQRTLDDAARGGRAVLPALPIGPFRGDLDWPVAGTIAGAFGRQVDRRFHTAT
                     VSNGVRIRAAPAAPVTAIHEGTVVFASSFAGFGKLVIVDHGEAAFSLYGYLADIDVTS
                     GERVARDQVLGSVGAGPNGEPGLYFELRIDGKPVDPLQWLKRKMPPAP"
BASE COUNT          314 a          927 c          906 g          328 t
ORIGIN      
        1 cgtcttggtt gcgcccgtgt ccttgacgat cttcgcgatg agctcctgct tgttcatgcg
       61 tcttcctccg gtgtgagcat gcggatggcg ggcaagtgcc cgcccttgcc ccgctcgcgt
      121 ttatacgcaa cgggatctgg cgtgtcaaga cgattcacgc gctcacgccg ccatcttcgc
      181 gcggagccgc ttgacggctt cgtcgagcgt cgcgtcgccg gcgggcgcgg acgccccgaa
      241 ctccacgtcc ggctgggcca ccggcacgtc cggctgcagg ccccgctcgt ggatggccgc
      301 gccggccggc gtgaggtacc acgcgtggga catccacagc gccgcgccgt ccgggagcgg
      361 gaacagccgc tggcgcgcgg cccggccggc cgtccgctcg ccgagcgtct cggcccgctt
      421 gttgccggag agcgccgcgg taaacagctc cgccgcgccc gccgtgccga agtcggtgag
      481 cacggcaatg ggcagcgtca cctcgccgtc ggcctcgcct gcggcgacga cctgccggct
      541 cgtgccgcgt ccgtccagga tcgccagcgt gccggcccgc acgaacagcc gggccatcgc
      601 caggccggcc tccagaggcc cgcgcgacgt gccgcgcagg tcgatcgcca gggccttggc
      661 gccggcctgc tgcagccgcc ggcacgcctc ccgcagcgac gccggcgttc ccgcggtgat
      721 gtccgggacg cgcacgtagc cgacggcgcc gtcgatgatc cgcgaggccg gcgccagcgg
      781 gaccagcgcc tcccgcgtca gcgtcatctc gtgcggctcc gcggcgttgc cgcggatgac
      841 gaggagcgag acggtgctgc ccggcgcgcc gcgcaggcgc cgcatgccct cccagacgga
      901 catctcgcgc gtcggctgcc gatcgatcgc gcggatgaag tcgccgggcg acagcccggc
      961 gcgggccgcc ggcgactggt cgcgcgccga gatgacccgg aggtagtact ggcgcgtcag
     1021 atcgaggcca acctcggccg gcccggcgtc ggcgcccgat tcgagcagcc gcgcatccgc
     1081 agccgtcaga tacgcgctgt cgggatcgag cgactccgca aggccgcgca tcgcgccgtg
     1141 catgagccga tcgacgtcga cccgctccac gtaattgtcc gtcgtcaggc tgaagacgtc
     1201 gtcgaagacg cgcaggtgcg ggtacgtctc gcccgccgcc cgccccttgc ccatgaggcc
     1261 gcccacgagg gcgaacacca gaacgggggc ggtgatgagg atgatggcga cgcgggtgcg
     1321 ggtggtcatg gagcgggggg catcttccgt ttcagccatt gtagtggatc gaccggcttg
     1381 ccgtcgatgc ggagctcgaa gtacagcccc ggctcgccat tcggtcccgc gccgacgctc
     1441 ccgagcacct ggtcgcgcgc gacacgctcg cccgacgtca cgtcgatgtc cgcgaggtag
     1501 ccgtagagcg agaacgcggc ctcgccgtgg tccacgatga cgagcttgcc gaacccggcg
     1561 aacgacgacg cgaagacgac cgtgccttcg tggatggcgg tcaccggcgc ggccggcgcg
     1621 gcgcggatgc gcacgccgtt cgacacggtc gccgtgtgga accggcgatc gacctgccgg
     1681 ccgaaggcgc ccgcgatcgt gccggccacg ggccagtcga ggtcgccccg gaacgggccg
     1741 atgggcagcg cgggcaggac ggcgcggccg ccccgcgcgg cgtcgtccag cgtccgctgc
     1801 agcgcctggc gcgcctcctc cagttcgcgc acgaaccgct cggccaggtc gcgccgggcg
     1861 tcgatgctcc ggatgaggcc ttcgtgggcg gcgacggcgg ccgccgccgc gtcccgggcg
     1921 cgggccacct cggcctgcgt ccgcgcggcc tcctcgcgcc tggcctcgat ggccgactgg
     1981 gccttccgga gctcggcaag cgtccgccgg tgttcggcga cgcgcgcgcg atcggcgccc
     2041 tggagccccg acacgaaacg gtaggcgcgc cccatctcgc gcaggtcgtc cacgttcagc
     2101 agcaggcgaa gatagccgcc gttgccgatc ttgtagagct ccaccagccg ggccgacagg
     2161 tcgggcagct gggaccgggc cgactgctcg agctcgaggg cacgggccgt cgtccgatcg
     2221 aggtcggcct ggacggcctc gagttcccgc gtgcgcaggt cggcctcagc cgcgcgaaga
     2281 tcgcgctcga cctcgagctt gcggagctcc tccacgagcg tccgcccgcg cgacgacagg
     2341 ctctcggctt cgcgccggag gctctggatg cgggctgccg cgcgccgatc gagggcgtcg
     2401 gcctgcgccc tgccgggcgc ctgggcggcg gcaccgcccg gcgcgagaag cgccccgcac
     2461 aggaggagcc cggcg
//
LOCUS       DAOSIF010000457         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_36765_length_2470_cov_2.838095, whole genome shotgun sequence.
ACCESSION   DAOSIF010000457 DAOSIF010000000
VERSION     DAOSIF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36765_length_2470_cov_2.838095"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLT35_12485"
     CDS             complement(<1..948)
                     /locus_tag="PLT35_12485"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOG30088.1"
                     /translation="MMNRAAVRKALLGVLAVLFVGASGKSFSDHRRLAEPVVLGPGVT
                     RVARLADYHATLRGTANDINLYVLEGREPGGTILVFGGTHAEEPAGRLAAWLIVEHAT
                     VDKGRLIVALSANRSASTVTRPGAAYPPTYTIPTAWGGQAFRMGDRWSSPLDQWPDPE
                     VFIHYPSGQYLAYSDIRNLNRTFPGRPNGTLTERTAFAFMELIRREGVDVAIDLHEAE
                     LQYPVINTIVTHQKGQEFATMVSMMLTDMEGFSIGTEFSPLNLHGLSHREIGDHSQAV
                     SLLFEAPEPFLDATRGRTDADVLLSGQDEFVVKAGAHGLL"
     gene            complement(959..1123)
                     /locus_tag="PLT35_12490"
     CDS             complement(959..1123)
                     /locus_tag="PLT35_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30089.1"
                     /translation="MITLQTVITFAYYLLTAGAAALLIGNFVRTKQWDRELLYLIVLV
                     PFLLRVLRLK"
     gene            complement(1241..>2470)
                     /locus_tag="PLT35_12495"
     CDS             complement(1241..>2470)
                     /locus_tag="PLT35_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30090.1"
                     /translation="RALRLSTELAMVAAALGGAVAGGHLFPARQIAEGAATYLDINLI
                     FITATLFMNLLKASGGVAFVVRGILRRFHRRRALLLAMLALVLLVPGALTGAGSVTVL
                     IAGGMVATVLGYMGISRVRVAAIVFVVASLSAAAPPVSLWAMMTAAGVNMPYVGFFWP
                     LLVPCLAAALFTVFALGWRGTAADLECALAELPEAPPGMSWWRVLAPFAVFLGLVWMG
                     RQWPHDTPVVGLPLMFAASALVSWGLSPVPVRVLRVASDTIEQLLPLLATLTCAGILV
                     QIMTLTGARGLLAVTAVTLPVAVVYATLFLVLPASEAVLMWGSAPVIGVPLVLLFNTI
                     GLNPIVALAGMSIIWPLGDALPPTAIIGRLTVDVVGYKGPYGGFLRACAIPALVMAVI
                     GTLMVVYSAKLAWLTGI"
BASE COUNT          420 a          848 c          892 g          310 t
ORIGIN      
        1 gagcagcccg tgcgcaccgg ctttgacgac gaactcgtcc tgcccgctga ggagcacgtc
       61 ggcgtcggtg cgcccccggg tggcgtcgag gaacggctcg ggcgcctcga agaggagcga
      121 gacggcctgc gagtggtcgc cgatttcgcg gtgcgagagg ccgtgcaggt tcagcggcga
      181 gaactccgtg ccgatagaga agccttccat gtccgtcagc atcatcgaga ccatcgtggc
      241 gaactcctgg cccttctggt gcgtgacgat cgtgttgatc acgggatact gcagctcggc
      301 ctcgtgcaga tcgatggcga cgtccacgcc ttcgcgccgg atgagctcca tgaacgcgaa
      361 ggcggtgcgc tcggtcaggg tgccgttggg tcggcccggg aaggtgcggt tcaggttgcg
      421 gatgtccgaa tacgcgaggt actgcccgct cgggtagtgg atgaacacct cggggtcggg
      481 ccactggtcc agcgggctgc tccagcggtc gcccattcgg aacgcctggc cgccccaggc
      541 cgtcgggatc gtgtacgtgg gcggataggc ggcgcctggc cgcgtcacgg tggacgcgct
      601 gcggttggcg ctcagggcga cgatcaggcg gcccttgtcg acggtggcgt gctcgacgat
      661 gagccacgcg gcgaggcggc cggccggctc ctccgcgtgg gtgccgccga agacgaggat
      721 cgtgcccccg ggctcccggc cctcgagcac gtagaggttg atgtcgttgg cggtgccgcg
      781 cagcgtcgcg tggtagtcgg cgagcctggc gacgcgggtg accccgggcc cgagcacgac
      841 gggctcggcg aggcgacggt gatcggagaa gctcttgccc gacgccccga cgaagaggac
      901 cgcgagcacg cccagcaggg ccttgcgcac agcggcgcgg ttcatcatgt cgtcgccctc
      961 acttcagccg cagcacgcgc agcaggaacg ggaccaggac gatcaggtag agcagctccc
     1021 ggtcccactg cttcgtcctc acgaagttgc cgatgagcag cgcggccgct ccggctgtca
     1081 gcaggtagta cgcgaaggtg atgacggtct ggagggtgat catgagcacc ttcgaaagac
     1141 aaaaagccgg gtttcagcac tcagggcccg atggggcctc ccgccgcacg tcgcgctctc
     1201 ccggctgaag agcgccgagc cccgtcgtcc cccgccccga tcagatgccc gtgagccacg
     1261 ccagcttcgc gctgtagacc accatcagcg tgccgatgac cgccatgacg agcgccggga
     1321 tcgcgcacgc tctcaggaac ccgccgtagg gccccttgta gcccacgacg tccacggtca
     1381 ggcggccgat gatggccgtc ggaggcagcg cgtcgcccag cggccagatg atgctcatgc
     1441 ccgccagggc cacgatcggg ttgaggccga tggtgttgaa gagcagcacc aggggcacgc
     1501 cgatcaccgg cgccgatccc cacatcagca cggcctcgga cgccggaaga accaggaaca
     1561 gcgtcgcgta caccacggcg acgggcagcg tgacggccgt caccgccagg aggccccgcg
     1621 cgccggtgag cgtcatgatc tgcacgagga tgcccgcgca ggtcagcgtc gcgagcagcg
     1681 gcagcagctg ctcgatggtg tcgctcgcga cgcgcaggac gcgcaccggc accggcgaca
     1741 ggccccacga cacgagggcc gaggccgcga acatgagcgg cagcccgacg accggcgtgt
     1801 cgtgcggcca ctggcggccc atccagacca gtccgaggaa caccgcgaaa ggcgcgagca
     1861 cccgccacca gctcatgccg ggcggcgcct cgggcaactc ggcgagcgcg cactcgaggt
     1921 cggcggccgt gcccctccat cccagcgcga acacggtgaa caacgcggcg gcgaggcacg
     1981 gcacgagcag gggccagaag aagccgacgt acggcatgtt cacgccggcc gccgtcatca
     2041 tggcccacag gctgacggga ggcgccgcgg cgctcaagct cgcgacgaca aagacgatcg
     2101 ccgcgacgcg cacgcgcgag atgcccatgt acccgaggac ggtggccacc atgccgccgg
     2161 cgatgagcac cgtcacgctg ccggcgcccg tcagcgcccc gggcacgagc aggacgaggg
     2221 ccagcatcgc aagcagcagg gcgcgccggc ggtggaagcg ccgcaggatg ccgcgcacca
     2281 cgaacgccac gcccccggac gccttgagca ggttcatgaa cagcgtcgcg gtgatgaaga
     2341 tcaggttgat gtcgaggtag gtcgccgcgc cctcggcaat ctgacgcgcg gggaagaggt
     2401 ggccgccggc caccgcgccg ccgagcgccg cggcgaccat cgccagctcg gtggacaggc
     2461 ggagcgcccg
//
LOCUS       DAOSIF010000458         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37002_length_2458_cov_4.971286, whole genome shotgun sequence.
ACCESSION   DAOSIF010000458 DAOSIF010000000
VERSION     DAOSIF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37002_length_2458_cov_4.971286"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1085
                     /gene="cpaB"
                     /locus_tag="PLT35_12500"
     CDS             228..1085
                     /gene="cpaB"
                     /locus_tag="PLT35_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOG30091.1"
                     /translation="MNRGVRVLLVLLVAIAMAGIASYAVYQGIQRIPAREVEVASQPV
                     VVAAKALPVGALIGPGDVKLAAWPSKSMVAGAFAAIDDVAGRGLLAAVLENEPITTAK
                     LAPREAGAGLAPTIPLGMRAVSVRVNDVIGVAGFVVPGSRVDVVATVNAGDSSITRTV
                     VSNLQVLAAGTRVDQEEAKDGKPIPTTVVTLLTTPEDAERVTLASGEGQILLVLRNPL
                     DIAPTTTAGVRLASLVGPPSPPPVEASFAGRRMVVAAPPAPPPPPPPPPYTVEAIRGA
                     KRTTEEVKK"
     gene            1082..2458
                     /locus_tag="PLT35_12505"
     CDS             1082..2458
                     /locus_tag="PLT35_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HOG30092.1"
                     /translation="MIAHRLFAPLAALCLAAGVAGAQAPTAPAPAADIERVVLAAGRS
                     TVLSTDFDITRIAVTNPAVADAVVVQPREVLIDGKSAGTVSLIIWGAARRAQYDVVVE
                     LGVSTLQQRLQALFPGEDINATLNEEALILSGRVSSTSVMLRAGEIAQASASKVKVIN
                     LLQLPGPPGSQQVMLQVRFAEVNRRAVQELGASWFMGANSQNDYAARIATQQFSAPVF
                     DGATTTFSDFLNLFVLNTKYNIGAVIKALQTHGYFQSLAEPNLIAYNGQEASFLAGGE
                     FPVPVVQGLTNAVTIEWKEFGVRLTFTPTIAGDMIRLKVKPEVSSLDFNNGITLQGFR
                     IPSLITRRAETDVELRDGQSFAIAGLLQNMTQDSASKIPILGSIPIIGNLFRSRADSK
                     EQTELMVLITPQLVRPLDPDEVPALPTMPARFLRSPGVGLPLEGGGGPADAPALPPAT
                     VIKKGPRE"
BASE COUNT          384 a          849 c          868 g          357 t
ORIGIN      
        1 gggaagatcc ttcgcgtgtg gagcatcgtc ggcgtgcagc cggtcgaggg gctgaccctc
       61 cgggacaccg cgagcccgag gctgccctac gcgctgccgc tcgcggcagg cgcgctggtg
      121 acgttgtgga tgagctagga gatcgcgcac catccggacc ggccgcccgc gaggcggcct
      181 ccgccatgta cgcggtcgcg ccggctgact gacgtgagga gtggcgtatg aaccggggtg
      241 tccgcgtcct gctcgtgctg ctcgtggcga tcgcgatggc gggaatcgcc agctacgcgg
      301 tgtaccaggg catccagcgc atccccgccc gcgaggtcga ggtggccagt cagcccgtgg
      361 tcgtggccgc gaaggcgctg ccggtcggag cgctgatcgg cccgggcgac gtgaagctgg
      421 cggcctggcc gtcgaagagc atggtggcgg gcgcatttgc cgccatcgac gatgtcgccg
      481 ggcgcggcct gctggcggcg gtcctcgaga acgaaccgat cacgaccgcg aagctggcgc
      541 cgcgggaagc gggcgccggc ctggcgccta cgattcctct gggcatgcgc gcggtctcgg
      601 tgagggtgaa cgatgtcatc ggcgtggcgg gcttcgtcgt ccccggatcg cgcgtggatg
      661 tggtggccac cgtcaacgcg ggcgactcgt cgatcacccg cacggtggtc agcaacctcc
      721 aggtgctggc ggccggcacg cgcgtcgacc aggaggaagc caaggacggc aagcccattc
      781 ccacgaccgt ggtgacgctg ctcaccacgc ccgaggacgc cgaacgcgtg acgcttgcgt
      841 caggtgaagg ccagattctc ctcgtgctcc ggaaccctct cgacatcgcg ccgacgacga
      901 ccgccggcgt gcgcctggcc tcgttggtcg ggccgccctc gcctccgccc gtcgaggcgt
      961 cgttcgcggg ccggaggatg gtcgtggcgg ccccgcccgc tccgcccccg ccgcctccgc
     1021 cgcctcccta tacggtggag gcgattcgag gcgcgaaacg cacgacggag gaggtgaaga
     1081 agtgatcgct cacaggctct tcgcgccgct cgccgcgttg tgcctcgccg ccggcgtcgc
     1141 cggcgcgcag gcgccgaccg cgccggctcc cgcggcggac atcgagcgcg tggtgctggc
     1201 cgccggccgc tcgacggtgc tctcgacgga cttcgacatc acccgcatcg cggtgaccaa
     1261 cccggccgtg gccgacgcgg tcgtcgtcca gccgcgtgag gtgctgattg acggcaagag
     1321 cgccggcacg gtgagcctga tcatctgggg cgccgcacgc cgcgcgcagt acgacgtcgt
     1381 cgtcgagctg ggcgtgagca cgctgcagca gagactgcag gcgctgttcc ccggagagga
     1441 catcaacgcg acgctcaacg aggaggcgct gattctctcg ggccgcgtct cgagcaccag
     1501 cgtgatgctc agggccggcg agatcgccca ggcgagcgca tcgaaggtga aggtgatcaa
     1561 cctgctgcag cttcccggcc cgccaggcag ccagcaggtg atgctgcagg tgcgcttcgc
     1621 ggaggtgaac cggcgggccg tccaggagct cggcgcgagc tggttcatgg gcgccaactc
     1681 gcagaacgac tatgccgcgc gcatcgcgac ccagcagttc agcgcgcccg tcttcgacgg
     1741 ggctaccacc accttcagcg acttcctgaa cctcttcgtc ctcaacacga agtacaacat
     1801 cggcgccgtg atcaaggccc tccagaccca cggctacttc cagagcctgg ccgagccgaa
     1861 cctcatcgcg tacaacgggc aggaggccag ctttctggcc ggcggcgagt tccccgttcc
     1921 cgtcgtgcag ggcctgacca acgcggtgac gatcgagtgg aaggagttcg gcgtgcggct
     1981 gaccttcacg cccacgatcg cgggcgacat gatccgcctc aaggtcaagc cggaagtgag
     2041 cagcctggac ttcaacaacg gcatcacgct gcagggattc cggatcccct cgctcatcac
     2101 gcgccgcgcg gaaaccgacg tcgagctgcg cgacgggcag tcgttcgcca tcgcgggcct
     2161 gctgcagaac atgacgcagg acagcgcgtc gaagatcccg attctcggca gcatcccgat
     2221 catcggcaac ctgttcagga gcagggcgga cagcaaggag cagaccgagc tgatggtgct
     2281 catcaccccg cagctggtcc ggccgctcga tcccgacgag gtgccggccc ttccgacgat
     2341 gccggcccgc ttcctgagga gtccgggtgt aggattgccg ctagagggcg gaggcgggcc
     2401 ggccgacgcg cccgccctcc cgccggcgac cgtgatcaag aagggaccga gggagtag
//
LOCUS       DAOSIF010000459         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37067_length_2455_cov_3.472917, whole genome shotgun sequence.
ACCESSION   DAOSIF010000459 DAOSIF010000000
VERSION     DAOSIF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37067_length_2455_cov_3.472917"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="gatB"
                     /locus_tag="PLT35_12510"
     CDS             complement(<1..858)
                     /gene="gatB"
                     /locus_tag="PLT35_12510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOG30093.1"
                     /translation="MHPAGTGGGRGYEPVIGLEVHAQLASSSKMFCGCAARGGAPPNS
                     RTCPTCLGLPGALPVVNARCVDLGVLVALALGCTVAERSVFARKHYFYPDLPKGYQIT
                     QFDAPLARDGAVSWVSDGRRRTVRVTRVHLEEDAGKSFHAGFPDSASSAYVDFNRSGV
                     PLVEVVTGPDLSDPADAAEFVRRLRAVLVAIGASEAIMEDGGLRCDANVSLRRAGGRA
                     PGARTEIKNLNSFRALQRALEYEIARQTAALDAGGAIQPDTLGWDEGSGRTVPMRAKE
                     QSDDYRYFPE"
     gene            949..2010
                     /locus_tag="PLT35_12515"
     CDS             949..2010
                     /locus_tag="PLT35_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOG30094.1"
                     /translation="MALSATSVGTAFVRRRSPAGRAALLLLPGLAAACAFGGAPGAGP
                     RVTVTPPRAVSPAPSADGVLTLRVGIVGERGVRILDLPLEDYVLAVVRAELPSGVLAD
                     PAAAGMLEVQAIVSRTFAIANLGRHRAEGFDVCGGTHCQVLRPPDAARAAPDGAARAV
                     AATRSLVIAHEGRAIQALFHAACGGHTASGASVWGGSEPYLAAVPDSFCSRLPAQAWT
                     FEADGASLARALASSPAMDVGTGLARIDITERDVSGRVTRVALTGSRTVSVRAEAFRG
                     ALRTAFGERSLRSTRFTVAQDGRRFIFQGTGLGHGVGLCQAGALERILAGVSPADVIA
                     HYFPGTRVMPADGVGRPAP"
     gene            2267..>2455
                     /locus_tag="PLT35_12520"
     CDS             2267..>2455
                     /locus_tag="PLT35_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOG30095.1"
                     /translation="MKAAVLAGQPEVAREAFGDLVTGCQRQATRLAFYLLRDASEADE
                     AVQDAFVKVYTHITSYRED"
BASE COUNT          310 a          914 c          887 g          344 t
ORIGIN      
        1 ctccgggaag tagcggtagt cgtcggactg ctctttcgcc cgcatcggga cggtgcgccc
       61 cgacccctcg tcccacccca gcgtgtccgg ctggatcgcg ccgcccgcgt cgagcgcggc
      121 cgtctgccgg gcgatttcgt actccagggc gcgctgcagg gcgcggaacg aattcaggtt
      181 cttgatctcg gtccgcgccc cgggcgcgcg gcctccggcc cggcgcagcg agacgttcgc
      241 gtcgcaccgc agcccgccgt cttccatgat cgcctccgag gcgccgatgg cgacgagcac
      301 ggcccgcagc cgccgcacga actcggccgc gtccgccggg tccgacaggt ccggccccgt
      361 gacgacctcg acaagcggca cgccgctgcg attgaagtcc acgtacgccg acgaggcgga
      421 atccgggaag ccggcgtgaa acgacttgcc cgcgtcctcc tccaggtgca cccgcgtcac
      481 gcgaaccgtc cgcctcctgc cgtcggaaac ccaggagacg gcgccgtcgc gcgcgagcgg
      541 cgcgtcgaac tgcgtgatct ggtagccctt cggcaggtcg ggatagaagt agtgcttcct
      601 ggcgaagacg gaccgctccg ccaccgtgca tccgagcgcg agcgccacga gcactcccag
      661 atcgacgcag cgggcgttca cgacgggcag cgcgccaggc agtcccaggc aggtcgggca
      721 ggtcctcgag ttcggcggcg cgccgccgcg cgctgcgcac ccgcagaaca tcttgctcga
      781 cgacgcgagc tgcgcgtgca cctcgaggcc gatgaccggc tcgtacccgc gccccccccc
      841 ggttccggct ggatgcatcg cgcgccacga aggagtatag ccgagcggcg acagcccggg
      901 cggcgcgggc ggtaatgggc ccggcggctg cgcccgatat gggcgagcgt ggccctgagt
      961 gcgacgtccg tcggcaccgc cttcgtccgg cgccgcagcc ccgccggcag ggccgccctg
     1021 ctgctcctcc cgggcctcgc cgccgcctgc gcgttcggcg gcgcgcctgg cgcgggcccc
     1081 cgcgtcaccg ttacgccgcc tcgcgccgtc tcgccggcgc cgtcggccga cggagtcctc
     1141 actctgcgcg ttggcatcgt cggcgagcgg ggcgtgcgca tcctcgacct ccccctggag
     1201 gactacgtgc tcgccgtcgt gcgcgccgaa ctcccgtcgg gcgtcctggc cgaccctgcg
     1261 gccgcgggca tgctggaggt ccaggcgatc gtgtcgcgga ccttcgcgat cgccaacctc
     1321 ggaaggcatc gcgccgaagg gttcgatgtg tgcggcggca cgcactgcca ggtccttcgc
     1381 ccgccggatg ccgcccgcgc ggcccccgac ggggcggccc gagccgtcgc ggcgacgcgc
     1441 agtctggtga tcgcgcacga gggccgcgcg atccaggcgc tgttccacgc cgcgtgcggc
     1501 ggccacaccg cgtctggcgc atcggtctgg ggcggcagcg aaccctacct ggccgcggtg
     1561 ccggattcgt tctgcagccg cttgcccgct caggcgtgga cgtttgaggc cgacggcgcc
     1621 agcctggccc gtgcgctggc ttccagccct gccatggacg tgggaacggg cctcgcccgg
     1681 atcgacatca cggaacgcga cgtgtccggc cgggtcacca gagtggccct gaccggctcg
     1741 cgcacggtga gcgtccgcgc cgaggcgttt cgcggggcgc tcaggacggc ctttggcgag
     1801 cgctcactgc gcagcacgcg cttcaccgtg gcccaagacg gccggcggtt catcttccag
     1861 ggcaccggcc tcggccacgg cgtggggctg tgccaggccg gggcgctcga acgcatcctt
     1921 gcaggcgttt caccggccga cgtcatcgcc cactacttcc cgggcacccg cgtgatgccc
     1981 gccgacggcg tcgggcgccc ggcgccgtga cccggcttcc gccggcgcgg accgacgagg
     2041 cgccctggat ctggtacgat acgctccgag cgccacccgc cgtccggccg gtaaacggaa
     2101 cccggcaggc tggcgtaaac aggggcaaga gcgagtgtcg cggccgtcgg attccggatt
     2161 cggcacggtg gcagggcccg agcgaggcag ccagcttgcg gagtacgttc gcccggcttt
     2221 accgggcgaa cggccggcgt cctcggtgtc cgaggtggcg cgacgcgtga aggccgcggt
     2281 gctcgccggg cagcccgagg tggcccgcga ggcgttcggg gatctggtca cggggtgcca
     2341 gcggcaggcc acgcgtctgg cgttttacct gttgcgggac gcgtccgagg ccgatgaggc
     2401 cgtgcaggat gcgttcgtga aggtgtatac gcacatcacg tcgtatcgcg aggat
//
LOCUS       DAOSIF010000460         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37130_length_2451_cov_4.591820, whole genome shotgun sequence.
ACCESSION   DAOSIF010000460 DAOSIF010000000
VERSION     DAOSIF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37130_length_2451_cov_4.591820"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PLT35_12525"
     CDS             complement(<1..1272)
                     /locus_tag="PLT35_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30096.1"
                     /translation="MSPIESLVAAVLKANGDGLVMHVGERPYVLAPTGSVNLSERPLT
                     VDAMKGALADLLAPEDQRTLNEVGAVEREIRPGDQTGDTFVLVAARGGDDIWIELRRQ
                     RAVTRQARPGLAGAAVSEEPLPAQAPAAKAEPRAVTDPSEAPPAAAQAAVAPSEAPPA
                     AAPAQPEPAALADRAAPAADTQAPQAPPARPAPPPTPESAPRPRMAPSHPPPSAPLPP
                     SYSVRHGLSGPPSARTDEPAVQPPSARRTGAVREPAPGVVLPLSRTPIRADAPRSSSS
                     PTPRYLGIGRLLRIASARGASSLFLTANARPSIRVDDEILPLETEPPLGKAEVEALIL
                     ELAPGASREAFAGGTAAEWVYELQDVGRVRCVSFRDQRGPGAVFRLIPTRLISADQLG
                     LSRDIQALCGEREGLVIVAGPWVFRLASELSD"
     gene            complement(1341..1766)
                     /locus_tag="PLT35_12530"
     CDS             complement(1341..1766)
                     /locus_tag="PLT35_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30097.1"
                     /translation="MRLDDRGWSGDGAFTRLVLEALKGDGGIEFIRVEDAPASRAESG
                     YAFISNEIYVKFARPPARRRWTRLLRPDPGPAGASGPALAALAARLSRLPDVGEPDYA
                     DDGLLQYLRTERITAPHQTRGIKLVELVRIYEAGAPARA"
     gene            complement(1878..>2451)
                     /locus_tag="PLT35_12535"
     CDS             complement(1878..>2451)
                     /locus_tag="PLT35_12535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG30098.1"
                     /translation="SARYGGPPIDDEAACWMVGEGAAVLVERVMAQAAQAAPPAVALQ
                     EFLALYDQRMFETTRPYPGTIETLQALAATHALALLTNKPEDSGRRILARLGLEPYFP
                     HAVFGDGRFAPKPDPAGLQWLMERCGATAGRTLLIGDSDVDVRTARNGGVRLCLARYG
                     FGFVRVDESMQRSDDWYLDQPSDLLTLLPQ"
BASE COUNT          338 a          886 c          891 g          336 t
ORIGIN      
        1 gtcgctgagc tcggaggcga gccggaagac ccacgggccg gccacgatga ccagcccctc
       61 ccgctcgccg cacagggcct ggatgtcccg ggacagcccg agctggtccg ccgagatgag
      121 ccgcgtcggg atcagccgga agacggcccc ggggccgcgc tggtcgcgga agctcacgca
      181 gcgcacgcgc ccgacgtcct gaagctcgta gacccactcc gccgccgtgc cgccggcgaa
      241 cgcctcgcgg ctcgcgccgg gcgccagttc gaggatcagc gcctccacct cggccttgcc
      301 gagcggcggc tcggtctcga gcggcaggat ctcgtcgtcc acgcggatgg acgggcgggc
      361 gttggcggtc agaaagagcg acgatgcgcc gcgcgctgac gcgatgcgga gcaggcggcc
      421 gatgccgaga taccgcggcg tcggcgagga cgaggagcgg ggcgcgtcgg cgcgaatcgg
      481 cgtgcgcgac agcggcagca cgacgccagg agccggctcg cgaaccgccc cggtccgccg
      541 ggcggacgga ggctgaacgg ctggctcgtc tgtccgggcc gacggcggac cggacaggcc
      601 gtgtctgacc gagtacgaag gcggcagagg cgcggacggc ggcgggtggc tgggtgccat
      661 gcgcggccgc ggcgcgctct cgggcgtcgg cggcggagcc ggtctggctg gaggggcctg
      721 gggcgcctgg gtgtcggcag ccggcgcggc tcgatcggcc agagcagccg gttccggctg
      781 ggcgggcgcg gctgcgggcg gcgcctccga cggcgccacg gccgcctgcg ccgccgcggg
      841 aggcgcctcc gacgggtccg tcaccgctcg cggctcggcc ttggctgccg gcgcctgggc
      901 cggcagcggc tcctccgaca ccgccgcgcc tgccagcccg ggacgtgcct ggcgagtcac
      961 cgcgcgctgg cgccgcagtt cgatccagat gtcgtcgccg ccccgcgcgg ctaccagcac
     1021 gaacgtgtcg cccgtctggt cccccggccg aatctcgcgc tcgaccgcgc caacctcgtt
     1081 cagcgtccgc tgatcctcgg gagcaagcag gtcggcgagc gcgcctttca tggcgtccac
     1141 cgtcagtggg cgctcagaaa ggttgacgga accggtcggc gccagcacgt agggacgctc
     1201 gcccacgtgc atgacgaggc cgtccccgtt ggccttcagg acggcggcga cgagcgattc
     1261 aatgggcgac acgtacctca tccggccctg gcggcctgct cccttcagat gtcggccgaa
     1321 aaggccggat tcattaggtt tcaggcccgt gccggcgcgc cggcctcgta gatgcgcacc
     1381 aactcgacga gcttgatgcc gcgcgtctga tggggcgcgg taatgcgctc ggttcgcagg
     1441 tactgcagga ggccgtcgtc ggcgtaatcc ggctcgccca cgtcggggag ccggctgagc
     1501 cgtgctgcca gagcagccag agcggggccg ctggcgccag cggggccggg gtcgggccgc
     1561 aagagccgcg tccaccgccg gcgagcgggc gggcgcgcga acttcacgta gatctcgttg
     1621 ctgatgaacg cgtagccgga ctcggcccgg ctggctggcg catcctcgac gcggatgaac
     1681 tcgatgccgc cgtcgccttt cagggcctcc aggacgagac gcgtgaacgc gccgtccccg
     1741 ctccagcctc tgtcgtccag ccgcacccct tcatgatatt ggggtcagat ccaggattac
     1801 ggggtcagat ccagaattcc tgtagtcctt caggaatttc cagacctggc cccgtaatcc
     1861 tggatctgac cccaatctca ctgcgggagg agggtgagga ggtccgatgg ttgatccagg
     1921 taccagtcgt cgctgcgctg catcgactcg tccacccgca cgaagccgaa cccgtaccgc
     1981 gccaggcaca ggcgcacgcc cccgttccgg gccgtccgga cgtccacgtc ggaatcgccg
     2041 atgagcagcg tcctgcctgc cgtggcgccg cagcgctcca tcagccactg cagccccgcc
     2101 ggatcgggct tcggcgcgaa ccgcccgtca ccgaacacgg cgtgcgggaa gtacggctcc
     2161 aggccgagcc gggccaggat cctccgcccc gagtcctcgg gcttgttcgt cagcagcgcc
     2221 agggcgtggg tggcggccag cgcctgcagc gtttcgatgg tgcccggata cggcctcgtc
     2281 gtctcgaaca tccgctggtc gtacagggcc aggaactcct ggagggccac ggccggcggc
     2341 gctgcctgcg cggcttgggc catcacgcgc tcgaccagca cggccgctcc ctccccgacc
     2401 atccagcacg cggcctcgtc gtcgattggc ggtccgccgt accgcgcgga c
//
LOCUS       DAOSIF010000461         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37192_length_2448_cov_3.229001, whole genome shotgun sequence.
ACCESSION   DAOSIF010000461 DAOSIF010000000
VERSION     DAOSIF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37192_length_2448_cov_3.229001"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLT35_12540"
     CDS             complement(<1..517)
                     /locus_tag="PLT35_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOG30099.1"
                     /translation="MAKQIQYGEQSRQAILRGVNKLADAVKITLGPKGRNVVLDKKFG
                     SPTITKDGVTVAKEIELKDPLENMGAQMVREVASKTSDVAGDGTTTATVLAQAIYREG
                     AKMVAAGSNPMDLKRGIEQAVEAIVADLQKRSKPVKGNMIAQVGTISANNDTTIGKII
                     AEAMDKVGKDGV"
     gene            complement(545..835)
                     /gene="groES"
                     /locus_tag="PLT35_12545"
     CDS             complement(545..835)
                     /gene="groES"
                     /locus_tag="PLT35_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOG30100.1"
                     /translation="MKVKPLHDRILVKRIEEGEQVVGGIIIPDTAKEKPQQGKVVAVG
                     AGKIDDKGKRVAMDVKADDTILFGKYSGQEIKLDGEEFLIMREDEVLGIIES"
     gene            complement(1060..1965)
                     /locus_tag="PLT35_12550"
     CDS             complement(1060..1965)
                     /locus_tag="PLT35_12550"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOG30101.1"
                     /translation="MDQALLHTRLDGLTPFRQGKVRDVYEVGDALLIVATDRISAFDY
                     VLASGIPDKGAVLNQLSAFWFDLMADIVPHHVLGTDPDAYPEAARRHAALLRGRSMLV
                     RPTRPVPIECVVRGYLSGSGWKEYQQTGSVCGVALPPGLRESDRLPEPIFTPSTKADS
                     GHDVNITEAQAAEIVGKNLLARLRDHSLAVFARGARHAEQLGIIVADTKLEFGMAREP
                     GGTERLLLIDEVLTPDSSRFWPADAYRPGGPQPSFDKQYVRDYLEAVGWNKQPPAPEL
                     PPDVVAKTREKYVEAFRRLTGRELS"
     gene            2102..>2448
                     /locus_tag="PLT35_12555"
     CDS             2102..>2448
                     /locus_tag="PLT35_12555"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG30102.1"
                     /translation="MHLRTAAVVLLAVVMVAWFLRGADMRAVWAAIQGGQLGLLALAV
                     GVTCLTYVFRAVRWQYLLRPLGRPPFSEVFKTTVIGFAVSTLVPARAGEVVRPYLLAR
                     RAGFSAASPFARI"
BASE COUNT          329 a          829 c          818 g          472 t
ORIGIN      
        1 tgacgccgtc cttgccgacc ttgtccatcg cctcggcgat gatcttgccg atggtcgtgt
       61 cgttgttggc cgaaatcgtg ccgacctggg cgatcatgtt gcccttgacc ggcttggacc
      121 gcttctgcag gtcggccacg atcgcctcga cggcctgctc gattccgcgc ttgaggtcca
      181 tcgggttgga tccggccgcg accatcttgg cgccttcgcg gtagatcgcc tgggccagca
      241 ccgtggcggt cgtcgtgccg tcgccggcga cgtcagaggt cttgctcgcc acctcgcgca
      301 ccatctgcgc gcccatgttc tcgagcgggt ccttcagctc gatctccttc gccacggtca
      361 cgccgtcctt ggtgatcgtg ggggagccga acttcttgtc gagcacgacg ttgcggccct
      421 tcgggccgag ggtgatcttg accgcatcgg ccagcttgtt cacgccgcgc agaatggcct
      481 ggcgcgactg ctctccgtac tgaatctgct tcgccatcgt taggactcct tcagcctcag
      541 cgcctcagct ctcgatgatt cccaggactt cgtcctcacg catgatgagg aactcctcgc
      601 cgtcgagctt gatctcctgc ccggagtact tgccgaacag gatcgtgtcg tcggccttga
      661 catccatggc gacgcgcttg cccttgtcat cgatcttgcc ggcgcccacc gcgacgactt
      721 tgccctgctg aggcttctcc ttggcggtgt ccggaatgat gatccctccg accacctgct
      781 cgccttcctc gatgcgcttc acgaggatcc ggtcatgaag tggcttgacc ttcatagtgt
      841 gccttcactc ctggtgaatt aggggaaccc gaccttgcca gcactcagcc gcactgtgtg
      901 ctagcactca ctacctgcga ctgctaatta tgaaagacgc acggcgaggc tgtcaagacg
      961 ggggctcgag ccgggggctc gggaaccgac agcgagacac cgcgctccat tccggattct
     1021 gagttccgga ttctgacttc tgacgtccga ctccagcccc taactcagtt cccgccccgt
     1081 gagccgccgg aacgcctcca cgtacttctc gcgcgttttc gccacgacgt cgggaggcag
     1141 ctccggggcg ggcggctgct tgttccagcc caccgcctcg aggtagtcgc gcacgtactg
     1201 cttgtcgaag ctcggctgcg ggccgccggg tcggtaggcg tccgccggcc agaacctcga
     1261 ggagtccggc gtcagcacct cgtcgatcag gaggagccgc tccgttccgc cgggttcgcg
     1321 ggccatcccg aactcgagct tggtgtcggc cacgataatg ccgagctgct cggcgtggcg
     1381 ggcgccccgg gcgaagacgg ccagcgagtg atcgcggagc cgggcgagca ggttcttccc
     1441 cacgatctcc gcggcctggg cctcggtgat gttcacgtca tggccgctgt cggccttcgt
     1501 cgacggcgtg aagatcggct ccggcaggcg gtccgactcc cgcaagccgg gcgggagggc
     1561 gacgccgcag acactgcccg tctgctggta ttccttccag cccgacccgg acaagtaccc
     1621 gcggaccacg cactcgatgg gcaccggcct cgttggccgg accagcatcg accggccgcg
     1681 gagcagcgcg gcgtggcgcc gcgccgcctc ggggtaggcg tccgggtccg tgcccaggac
     1741 gtggtgaggc acgatgtccg ccatgaggtc gaaccagaag gccgacagct ggttcaggac
     1801 tgcgcctttg tcggggatcc ccgaggcgag cacgtaatcg aaggccgaga tccggtccgt
     1861 ggcgacgatg agcagggcgt cgccgacctc gtagacgtcg cggaccttgc cctggcgaaa
     1921 cggcgtcagg ccgtcgagcc gcgtgtggag gagcgcctga tccatgggag acccccctgg
     1981 tggagggctg atcttaccgg gggcccgccg gaggttcaac tccgcgcggg gcggcgcggc
     2041 ggggccggtc cggtcgatag acgggccgtg cccgttgtag tagagtcgca tccggatgcg
     2101 tgtgcacctg cggaccgcgg cggtggtcct gctggccgtg gtcatggtgg cgtggtttct
     2161 ccgcggcgcc gacatgaggg cggtgtgggc ggcgatccag ggcgggcagc tcggcctgct
     2221 cgcgctggcc gtcggggtga cctgcctgac gtatgtcttc cgcgccgtgc gctggcagta
     2281 cctgttgcgc ccgctcgggc ggccgccctt ttccgaggtc ttcaagacga cggtgattgg
     2341 cttcgccgtc tcgacgctcg tccccgcccg ggccggtgaa gtggtgcggc cgtacctgct
     2401 ggcccggcga gccggcttca gtgccgcgtc cccctttgcg aggatcat
//
LOCUS       DAOSIF010000462         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37213_length_2447_cov_2.939381, whole genome shotgun sequence.
ACCESSION   DAOSIF010000462 DAOSIF010000000
VERSION     DAOSIF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37213_length_2447_cov_2.939381"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="PLT35_12560"
     CDS             <1..1727
                     /locus_tag="PLT35_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG30103.1"
                     /translation="SAQLCRYVKGPDFPTGGHVLNSPDELKDIYKTGSGTLRVRGTWE
                     PGPTTRGGRTIIITSIPYMVNKAQLVERIAEVVLGRKLPPLVDVKDISTDDVRIQLEL
                     KRDADEKMVMAYLCRHTPLQTTFPVNLTCLVPTENPEVGRPDRLDLKSILWHFLHFRL
                     DVVTRRLEHELAAVERRIHVLEGFERIFGVLDEIIRIIRKSDGKQDAAKKIMARFDLD
                     AGQTDAILELKLYRLARLEILAIQTELEDRRGRAKTIGLLLNNEHKRWGLVRDELEDL
                     QKRYSGPKADPRRTRIEAVEEVEYSASDFIVEEDGVVIVSRDGWVKRQKEVKDLAATR
                     LREGDAVLAVLPGSTRAAAVFFTNFGVAYTSAIADVPASTGYGEPIQRLFKFRDGERV
                     IAAVSLDPRVTRGIEPRKPGGVPPTHAVAVTSDGYSLRFGLEAFAEPSTRAGRRYARP
                     AGGAEVVGVAVIDGSETIIAATAQARAMLCKAEEINFLSGPGKGVILIKLDPKEDRVL
                     GFIASVGDRDLLTVETSRGAEQTISTAKYEVTGRGGRGRELLQRGQFTRVVPAPLASP
                     PPLAETPS"
BASE COUNT          402 a          807 c          904 g          334 t
ORIGIN      
        1 cgagcgccca gctctgccgc tacgtcaagg gcccggactt cccgaccggc ggccacgtgc
       61 tgaactcgcc cgacgagctg aaggacatct acaagacggg ttcaggcacg ctgcgcgtgc
      121 gcggcacgtg ggagcccggg ccgacgacac gcggcggccg gaccatcatc atcaccagca
      181 tcccgtacat ggtgaacaag gcccagctcg tcgagcgcat cgccgaagtg gtgctgggcc
      241 gcaagctgcc gccgcttgtg gacgtcaagg acatctcgac cgacgatgtg cggatccagc
      301 tggagctgaa gcgggacgct gacgagaaga tggtgatggc gtacctctgc aggcacacgc
      361 cgctgcagac aacgttcccg gtgaacctga cgtgcctggt gccgacggag aaccccgagg
      421 tcggcaggcc ggatcggctc gatctcaagt ccatcctgtg gcacttcctg cacttccgcc
      481 tcgacgtcgt cacgcgccgc ctggagcacg agctggcggc ggtcgaacgc cgcatccacg
      541 tgctcgaagg gttcgagagg atcttcggcg tcctcgatga gatcatccgg atcatccgga
      601 agtcggacgg caagcaggat gccgcgaaga agatcatggc ccggttcgac ctggacgccg
      661 ggcagacgga cgccatcctc gagctgaagc tctaccgact ggcgcgcctc gagatcctcg
      721 ccatccagac cgagctcgag gaccgtcgcg ggcgcgcgaa gacgatcgga ctgctgctca
      781 acaacgagca caagcggtgg gggctcgtgc gggacgagct ggaggacctg cagaagcgct
      841 actcggggcc gaaggcggac ccgcgccgca cgcgcatcga ggcggtcgaa gaggtcgagt
      901 actccgccag cgacttcatc gtcgaggagg acggcgtcgt catcgtgtcg cgcgacggat
      961 gggtgaagcg ccagaaggaa gtgaaggacc tcgcggcgac gcggctgcgc gagggcgacg
     1021 ccgtgctggc cgtgctgccc ggcagcacgc gtgcggccgc ggtgttcttc acgaacttcg
     1081 gcgtggccta cacatcggcc atcgctgacg tgccggcgtc caccgggtac ggcgagccga
     1141 tccagcggct gttcaagttc agggacggag agcgcgtgat tgccgccgtc agtctcgatc
     1201 cgagggtcac gcggggcatc gagccgcgga agccaggcgg cgtgccgccg acgcacgccg
     1261 tcgccgtcac gagcgacggc tacagcctgc ggttcggcct ggaggcgttc gcggagccca
     1321 gcacccgggc cgggcggcgc tacgcgcgcc ccgccggggg cgccgaggtg gtcggcgtcg
     1381 ccgtgatcga cggcagcgag accatcatcg cggcgacggc gcaggcgcgg gcgatgctct
     1441 gcaaagccga ggagatcaac ttcctgtccg gcccaggcaa gggcgtcatc ctcatcaagc
     1501 tggacccgaa ggaggaccgc gtgctcgggt tcatcgcgtc ggtgggggac cgcgacctgc
     1561 tgaccgtcga aaccagccgg ggggccgagc agaccatcag caccgccaag tacgaggtga
     1621 ccggccgcgg cggcagaggg cgcgagttgc tccagcgcgg ccagttcacg cgcgtcgtgc
     1681 cggccccgct cgcgtcgccg ccgccgctcg cggagacgcc gtcgtagctg gtgccgtgcg
     1741 ggccgccgct cgacgagccg aaaccgtagg gcggaggcgg gtgccacggt gacgaggtgc
     1801 ctccggagtc cgggtgccga atcgcatggg cgcctcggtt tgctgggatt cgaagatggg
     1861 cgcgcgtccg ttgttgcaat gaggtgcgag cagtcgctgc tcgggaccgg cagggccatg
     1921 gcctcccccg ggtgcaccgc ggcggaacca cggagcgcag ctggtcggcc accgcgcgct
     1981 aggccgcgtc tgcgggcctc ggacggcact gcctcatgac ggccgaggtc atcggatgta
     2041 ggatccgaag gggccgttct cggacgccga actcgccgtg cgcgggctgg caaggccccg
     2101 agggccgtcc ggtcactccg ggcattggag cggtgggatg agcgagagca ggcgctgacg
     2161 gcgcaggcga ttccggcaca tgcgtcgggc agggactgga ggcaagccag ccggcggcgc
     2221 gggtgggctg ccgccggccc gattgactgc gcgcagcact tccgaacccg tcaccgacaa
     2281 gccgcccgtc ttggcggccg catgcgtcag gctcggagta ggatagaacg tcgtgccggc
     2341 agggttcagc gataccgatc gggagagcct ccaggtgcag gtggacctgc tgcgccgggc
     2401 gacgccgagc cggcgtccct gtcctctccc gaaggtggtg ccgatgg
//
LOCUS       DAOSIF010000463         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37288_length_2443_cov_3.756281, whole genome shotgun sequence.
ACCESSION   DAOSIF010000463 DAOSIF010000000
VERSION     DAOSIF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37288_length_2443_cov_3.756281"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLT35_12565"
     CDS             complement(<1..299)
                     /locus_tag="PLT35_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30104.1"
                     /translation="MSYTVIARRWRPQRLEEVVGQRAVTRTLHNAIASGRIAQAFVFA
                     GPRGVGKTTTARILARMLNCEKGPTPDPCGACDAFREIADGRDMDVLEIDAASNTG"
     gene            complement(476..577)
                     /gene="ffs"
                     /locus_tag="PLT35_12570"
     ncRNA           complement(476..577)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLT35_12570"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            788..1069
                     /locus_tag="PLT35_12575"
     CDS             788..1069
                     /locus_tag="PLT35_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOG30105.1"
                     /translation="MSTGCVEDAELVERARLGDHAAFGTLVDRCQVPVFRAALAALGT
                     REDAEEASQEAFVAAFRHLQDYRGDATFKTWLLSIAWRKALSPCGSPGH"
     gene            1185..1412
                     /locus_tag="PLT35_12580"
     CDS             1185..1412
                     /locus_tag="PLT35_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30106.1"
                     /translation="MNVADGESGRIRLRASLDYQLLKDSPEPDMPAGKAAIARSVTAI
                     LSDGVSTVLSESADPLTDRKATLEVRATIVK"
     gene            1494..2078
                     /locus_tag="PLT35_12585"
     CDS             1494..2078
                     /locus_tag="PLT35_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30107.1"
                     /translation="MRRLLVLGLMALPLLVSGCSKGPAEAALKAADEAIAKAAPLAEK
                     FVPDQFKTLTDAAAAAKSSFDKGDYAAALAAAKDLPAKAGEILAAAGAKKDELTGLWN
                     GLAESLPAQLAGVTEKITAIAALKRLPKGIDAAGLETAKAELAEITGTWTRATEAFGT
                     GEIPSALQAANDVKARVEALVKTLEPVVLPPAKK"
     gene            complement(2152..>2443)
                     /locus_tag="PLT35_12590"
     CDS             complement(2152..>2443)
                     /locus_tag="PLT35_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30108.1"
                     /translation="ARLADDHRHARILAAGVATLEGVVLDPPEVDTNIVVFRMPSAAD
                     AEAFAAALEREGVLVSDFGGGRLRVVTHDGITEADCRAAIDAMRKVRAGMIA"
BASE COUNT          394 a          857 c          856 g          336 t
ORIGIN      
        1 cccgtgttcg aggcggcgtc gatctcgagg acgtccatgt cgcggccatc cgcgatctcg
       61 cggaaggcgt cgcacgcgcc gcacgggtcc ggcgtcggcc ccttctcgca gttgagcatg
      121 cgggcgagga tccgggcggt ggtcgtcttg ccgaccccgc gcgggccggc gaagacgaag
      181 gcctgcgcga tgcggccgct agcaatggcg ttgtggaggg tccgcgtgac ggcgcgctgg
      241 ccgacaacct cctcgagtct ctgcggccgc cagcggcggg cgatcacggt gtaggacatc
      301 ggcagaatac ggaattcaga atacagaact cagcatacag gagacagcag ccgggagtca
      361 gcggcgagtc ccgccgcggc ccgcttccag cggcgccagc cgcggcgctc tcagcggccg
      421 agccggcgcg gcccgacgcc ccgagccgcc gaagacgggc ggccgcgaac cccactgcgg
      481 cccgggttgt cctgcggcac atcacagggt ccgcttagtg ctgctgcctt ccggccctga
      541 cacggttcac aggctgcgat tgcacagggt ccgggccgca gcgcgattca cgaccgcccc
      601 taggagtgta ccctcatccg gggtcaggtc caagaaacag ggggtttacc gtgcaatagt
      661 gccacccctc gcgaaagacg ggccgcgcct ctggcccggg atcctgtgcc gtggcaccag
      721 aaaacggcaa ttcgcctgtt ttcgggacct gaccccactg gtggtcccaa ccggagcggg
      781 gaaacgggtg tctactggtt gtgtggagga cgccgaactg gtcgagcggg ccaggctggg
      841 cgaccacgcc gcgttcggga cgcttgttga ccgctgtcag gtcccggtct tccgggcggc
      901 actggccgcc ctcggcacgc gcgaagatgc ggaggaagcc tcccaggagg cgttcgtggc
      961 ggcgttccgc cacctccagg attaccgggg agacgccacg ttcaagacct ggcttctctc
     1021 catcgcctgg cgcaaggccc tcagcccttg tggaagtccc ggccattgac gtcccggctt
     1081 cggacgcggc gggacccgat cgctcggatg acgatcccag gaaggagtat cagccataac
     1141 gacccgttct ctcgctgccg ccgccggtca ggaagaccat cacaatgaac gtggcggacg
     1201 gcgagagcgg ccggatccgg ctgcgcgcct cgctcgacta ccagctcctg aaggactccc
     1261 ccgagcccga catgcccgcc ggcaaggcgg cgatcgcgcg aagcgtgacg gcgatcctga
     1321 gcgacggcgt gtccacggtg ctcagcgagt cggccgaccc gctcacggac cgcaaggcca
     1381 cgctcgaggt gagggcgacg atcgtcaagt agctccggct cctggcccgc gattgcgcta
     1441 tgatgggcag ttgtccctgc cgcccccgcg gatccttcac gtcgaggtgt gacatgcgca
     1501 gactgctggt gctcggattg atggcgttgc cgcttctcgt atcgggctgc agcaagggcc
     1561 cggccgaagc cgcgctgaag gcggccgacg aggccatcgc caaggcggcc ccgctcgccg
     1621 agaagttcgt gcccgaccag ttcaagacgc tgaccgacgc cgcggcggcg gccaagagca
     1681 gcttcgacaa gggcgactac gccgccgcgc tggcggccgc gaaagacctg ccggcgaagg
     1741 ccggcgagat cctggctgcc gcaggcgcca agaaggacga gctgaccggc ctctggaacg
     1801 gcctcgccga atcgcttccg gcgcagctcg cgggcgtgac cgagaagatc accgccatcg
     1861 ctgcgctgaa gcggctgccc aagggcatcg acgccgccgg cctcgagacc gcgaaagcgg
     1921 aactcgccga gatcaccggc acgtggacca gggccaccga ggcgttcggc accggcgaaa
     1981 tcccgagcgc gctgcaggcg gcgaacgacg tgaaggccag ggtcgaggcc ctcgtgaaga
     2041 cgctcgagcc ggtcgtcctg ccgccggcga agaagtaagt cggctcggga ttcgaggccg
     2101 ggggcccggg gccgatggcc cggatcgcgc gcgtggccgc ccgccgggcg gtcacgcgat
     2161 catgcccgcc cgaaccttcc gcatcgcgtc gatggcggcg cggcaatccg cctcggtgat
     2221 gccgtcgtgc gtcaccaccc tcaggcggcc gccgccgaaa tcggaaacca gcacgccttc
     2281 gcgctccagc gcggccgcaa aggcctcggc gtccgccgcg ctcggcatgc gaaagacgac
     2341 gatgttcgtg tccacctcgg gcgggtccag cacgactccc tcgagcgtcg cgacgcccgc
     2401 tgcgaggatg cgcgcgtggc ggtggtcgtc ggccagccgc gcc
//
LOCUS       DAOSIF010000464         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37333_length_2441_cov_3.179799, whole genome shotgun sequence.
ACCESSION   DAOSIF010000464 DAOSIF010000000
VERSION     DAOSIF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37333_length_2441_cov_3.179799"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="PLT35_12595"
     CDS             complement(<1..1670)
                     /locus_tag="PLT35_12595"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOG30109.1"
                     /translation="MARRIAFAASAVAAAIAAWAAATMPPRAVRLAGGEAAGGPFVVR
                     GAYHVHSRASDGTGSAMDIAAAAARAGLRFVILTDHGDGFRRTAPPRYIGAVLCVEGV
                     EISTTGGHYAAIGMAPAPYPLGGEPRDVVEDVARLGGFGVVAHPDSPKPALRWDEWDA
                     PVDAIEWLNADSEWRDEARWRLLPALLHYPWRPAETVVSLFDRPEGTIRRWDRLAQAR
                     PVVGLAGADAHARLGFGGKTAPYDEACYIKAPSYEAAFRAFSLGVELPAPLNGDAEAD
                     AAAVVAAMRAGHVYTAIDAVAGPAFLEFTAESGGRVARQGDRLAFAGEVTIRARINGP
                     PGSSLSLVGNGSLVTSAPGPALTWRGSRPGVYRVEAHAPGSPGRPALPWIVSNPIYVG
                     GPLGPPVRPERPPVRDRLVWPPGSWDVEKDPSSTGALASPAGELLRRTFRFRLGAGQA
                     SPFVAFVTRDVGPLRDAAQLAFRAAASRPLRMSVQVRTVERGVERRWQRSVYLDTDPR
                     DIVVPLESMRTAGAGERLAFDPRRIELLLLVFDTVNARAGDGGVAWIEG"
     gene            complement(1695..>2441)
                     /locus_tag="PLT35_12600"
     CDS             complement(1695..>2441)
                     /locus_tag="PLT35_12600"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HOG30110.1"
                     /translation="LTRPQLEAFVRGLLSGGLSPRSAARVVAAVRGFYRYLVREGSLP
                     ASPAEDLQGARPWPALPKHLSSDEVDRLIERPDISTPLGLRDRAMIEVLYATGLRVSE
                     LVGLRARDVSLSGGYLTCTGKGGRQRLVPLGDRAVSCLRQYQARARPELARPSSPPRL
                     FLNARGGALTRVGFWKLLKGYARQAGLTGKVSPHVLRHSFATHLLEHGADLRAIQLML
                     GHADVSTTQIYTHVHEARMRAVYDRFHPRR"
BASE COUNT          343 a          879 c          926 g          293 t
ORIGIN      
        1 ccctcgatcc aggcgactcc cccgtctccg gctctggcgt tcacggtgtc gaacaccagc
       61 agcagcaact cgatgcggcg cgggtcgaag gcgaggcgct cgccggcgcc tgccgtgcgc
      121 atgctctcga ggggcacaac gatgtcgcgc gggtcggtgt cgagatacac cgagcgctgc
      181 cagcgccgct cgacgccgcg ttcaacggtg cggacctgca cggacatccg cagcggccgg
      241 ctggcggcgg cccggaacgc gagctgcgcc gcgtcgcgaa gcgggccgac gtcgcgggtg
      301 acgaacgcga cgaacgggct cgcctgcccg gccccgagcc ggaagcggaa tgttcgcctg
      361 agcagctcgc ctgccggcga cgcgagcgcg cccgtcgatg acgggtcttt ctcgacgtcc
      421 cacgacccgg gaggccacac gagccggtcg cgcaccggcg ggcgttccgg gcggactggc
      481 ggacccaggg gcccgcccac gtagatcgga ttgctcacga tccacggcag cgcgggccgg
      541 ccggggctgc ccggggcgtg ggcctcgacg cggtagacgc cgggccggct ccctcgccac
      601 gtcaaggccg gccccggcgc cgacgtgacg agggagccgt tcccgacgag cgacagcgag
      661 ctgcccggcg gaccgttgat ccgggcgcgg atcgtgacct cccccgcgaa cgcgagccga
      721 tcgccctggc gcgcgacgcg cccgccggac tcggcggtga actcgaggaa cgcggggccg
      781 gcgacggcgt cgatcgccgt gtacacgtgt ccggcgcgca tcgccgctac caccgccgcc
      841 gcgtccgctt cggcgtcgcc gttgagcggc gccggcagct cgactcccag agagaacgcc
      901 cggaacgccg cctcgtacga cggcgccttg atgtagcacg cctcgtcgta cggggcggtc
      961 ttgccgccga agcccaaccg cgcgtgggcg tcagcgcccg ccagcccaac gacggggcga
     1021 gcctgcgcga ggcggtccca gcgccggatc gtgccctcgg ggcgatcgaa cagggagaca
     1081 accgtttcgg caggccgcca ggggtagtgc aggagcgcgg gcagcagcct ccagcgcgct
     1141 tcgtcgcgcc actcggagtc cgcgttgagc cactcgatcg cgtcgacggg cgcgtcccac
     1201 tcgtcccacc gcagcgcggg tttcggggag tccggatgcg ccaccacgcc gaatccgccg
     1261 aggcgcgcga cgtcctcgac gacatcgcgc ggttcgcccc cgagcgggta cggggcgggc
     1321 gccatgccga ttgccgcgta gtggccgccg gtggtgctga tctcgacgcc ctcgacgcac
     1381 agcaccgcgc cgatgtagcg cgggggcgct gtgcggcgga agccgtcgcc gtgatcggtc
     1441 aggatcacga accggaggcc cgcgcgcgcg gccgcggccg cgatgtccat cgccgagccg
     1501 gtgccgtccg acgcgcgtga gtgcacgtga taggcgccgc gcacgacgaa agggccgccg
     1561 gcggcttccc cgccggcgag gcggaccgcc cggggcggca tcgtggctgc ggcccaggcg
     1621 gcgatggccg cggcaacggc ggaggcggcg aatgcgatgc gacgagccat cgaggctgga
     1681 ctccccgctc gatcctaccg cctcgggtgg aatcggtcat acaccgcgcg catccgcgcc
     1741 tcgtggacgt gcgtgtagat ttgggtggtg gacacgtcgg cgtggccgag catcagctgg
     1801 atggcccgga gatcggcgcc gtgctccagc agatgggtgg cgaaggagtg ccgaagtacg
     1861 tgcgggctga ccttgcccgt caggccggcc tgccgggcgt agcccttcag cagcttccag
     1921 aacccgactc gtgtcagcgc ccctcccctc gcgttcagga acagccgcgg cggggacgaa
     1981 gggcgagcca gttccggcct ggcccgggcc tggtactggc gcaggcacga gacggcgcga
     2041 tctcccaggg ggacgaggcg ctgcctgccg cctttccccg tgcacgtcag gtagccgccc
     2101 gagaggctca cgtcacgcgc gcgcaggccg acgagttccg acacgcgcaa gccggtcgca
     2161 tacagcacct cgatcatggc ccggtcgcgg agccccagcg gcgtcgagat gtcgggccgc
     2221 tcgatcaggc ggtcgacctc gtccgaggag aggtgcttgg ggagcgcggg ccagggccgg
     2281 gcgccctgca ggtcttcggc cgggctcgcg ggcagggacc cttcgcggac gaggtagcgg
     2341 tagaacccgc ggacggcggc aacgacgcgg gcggcggacc gcggcgagag gccgccggac
     2401 aggaggccgc gcacgaaggc ttcgagctgg ggccgtgtga g
//
LOCUS       DAOSIF010000465         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37470_length_2434_cov_4.671290, whole genome shotgun sequence.
ACCESSION   DAOSIF010000465 DAOSIF010000000
VERSION     DAOSIF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37470_length_2434_cov_4.671290"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PLT35_12605"
     CDS             <1..954
                     /locus_tag="PLT35_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30111.1"
                     /translation="RSALAKLAQIDSRGSTDLHGGWAAGCSQLAAHLGAADVGRCLLL
                     TDGLANVGIDEPEQLVSLAGAARREGRIATSTFGVGADFDERLLRGMADAAGGRFYFI
                     EQPAQIPDLLTSELGELLQVVARDASLILQLPQGVGASPLNALETADITGGIQVRLGD
                     LVSGQQFDVVVALKFPAGHAGDTIAVQFTLADRDGALSAPPQVIGWSYGAASSLQGQP
                     RDRIVDRAVARLYTARARAEALELNREGRFREARARLEKTARRILGYAGDDEELRQMA
                     EELRAEAADYSRGMDEMARKQRYFRSYSSQKDRLPDGKARR"
     gene            complement(995..>2434)
                     /locus_tag="PLT35_12610"
     CDS             complement(995..>2434)
                     /locus_tag="PLT35_12610"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG30112.1"
                     /translation="GRDLVNYTLAVAENNRLPLVAPGAAGLLGAGQRGAWATASQPAW
                     HKPEPAAVPLAPGDPSLVDHVVYIIKENRTYDQLFGDLPQGNGEPSFVLFGEGVAPNH
                     RRLARQFVLLDNFYATGGNSGDGHQWVTQANEADYAMWPGYTGRSYPFDGTDALAYAP
                     GGFIWDGARSRGKSVRIYGEFMPAERGVASKERAALLREWERGADFSGRWKPTPRVQG
                     MAEFVAPDFPAYTTAIPDGIRANLFLRDLRGWEASGTMPNLVLLQLPSNHTYGTSPGL
                     STPQAMVADNDLALGRVVEALTRSRFWPRMAIFVVEDDAQDGVDHVDGHRTVALAVSP
                     YVRRRQVDSTFYSHPSILKTIELMLGLPPLSIFDRIANDMRASFTDEPDLTPYTAVVP
                     KQSLHEVNPPAGALKGAARAAALASARMRFDVPDAAPTDRLNRTLWHAVKGWDVPYPG
                     VRRAVFAPLSIDVDDDEREDAKAAGRGRR"
BASE COUNT          354 a          865 c          880 g          335 t
ORIGIN      
        1 cggagcgcgc tggcgaagct cgcgcagatc gactcgcgcg gctcaaccga cctccacggc
       61 ggctgggcgg cgggctgcag ccagctggcc gcgcacctgg gcgcggcgga cgtcggccgg
      121 tgcctcctct tgaccgacgg cctcgccaac gtcggcatcg acgagccgga gcagctcgtg
      181 agcctggctg gcgcggcgcg ccgcgagggg cgcatcgcca cgagcacgtt cggcgtcggc
      241 gccgacttcg acgagcgcct gctccgcggc atggccgacg cggcgggcgg gcgcttctac
      301 ttcatcgagc agccggccca gatcccggac ctgctcacga gcgaactagg cgaactgctc
      361 caggtggtcg cacgcgacgc cagcctcata ctgcagctgc ctcagggggt cggggcgtcg
      421 ccgctcaacg ccctcgaaac ggcggacatc accggaggca tccaggttcg cctgggcgac
      481 ctggtgtcgg gccagcagtt cgacgtggtg gtggccctga agttcccggc cggccacgcg
      541 ggcgacacga ttgccgtcca gttcaccctc gcggaccgcg acggcgcgct ctcggcgccc
      601 ccgcaggtca tcggctggag ctacggcgcc gccagctctc tccagggaca gccccgcgac
      661 cgaatcgtcg accgcgcggt ggcccgcctg tacaccgcgc gcgctcgcgc cgaggcgctg
      721 gaactgaacc gcgagggccg gttccgcgag gcccgggccc gcctcgagaa gacggcccgg
      781 cgcatcctcg gctacgccgg cgacgatgag gagttgaggc agatggcgga ggaactgcgc
      841 gccgaggcgg ccgactactc gcgggggatg gacgagatgg cgcggaagca gcgctatttc
      901 cggtcctaca gcagccagaa agaccgcctg ccggacggaa aggcgaggcg ttgagcgggc
      961 gccggccgga cggttcggga caccggcgcg cggctcagcg cctgccgcgg ccagccgcct
     1021 tcgcgtcctc ccgctcatca tcgtccacgt cgatcgagag cggcgcgaag accgcacgcc
     1081 tgacgccagg gtacggcacg tcccagccct tgacggcgtg ccacagggtc cggttgagcc
     1141 ggtcggtcgg cgctgcgtcc ggcacgtcga accgcatgcg cgccgacgcc agcgctgccg
     1201 cgcgcgccgc gcccttcaac gcgcccgccg gcgggttcac ctcgtgcagc gactgcttcg
     1261 gcacgacggc ggtatagggc gtcaggtcgg gctcgtcggt gaagctcgca cgcatgtcgt
     1321 tggcgatgcg gtcgaagatc gacagcggcg gcaggccgag catcagctcg atggtcttca
     1381 ggatgctcgg gtgcgagtag aacgtcgagt ccacctggcg gcgccgcacg tacggactca
     1441 ccgccagcgc cacggtccgg tgcccgtcca cgtggtccac gccgtcctgc gcatcgtcct
     1501 cgacgacgaa gatcgccatg cgcggccaga aacggcttcg cgtcagcgcc tcgacgaccc
     1561 ggcccagcgc aagatcgttg tcggcaacca tcgcctgagg cgtcgagagg cccggcgacg
     1621 ttccgtaggt gtggttgctc ggcaactgca gcagtaccag gttcggcatc gtgccggatg
     1681 cctcccaccc gcggagatcg cgcaggaaga ggttggcgcg gatgccgtcg ggaatggccg
     1741 tcgtataggc gggaaagtcg ggggccacga actcggccat gccctggacg cgcggtgtcg
     1801 gcttccagcg gccgctgaag tccgcgccgc gctcccactc gcggagcagc gccgcgcgct
     1861 ccttcgaggc cacgccgcgc tccgccggca tgaactcgcc gtagatgcgg accgactttc
     1921 cgcgcgatcg cgcgccgtcc cagatgaacc cgcccggcgc gtaggccagc gcgtccgtcc
     1981 cgtcgaacgg atagctccgg ccggtgtagc cgggccacat cgcgtagtcg gcctcgttgg
     2041 cctgcgtcac ccactgatgg ccgtcgccgc tgttgccccc ggttgcgtag aagttgtcga
     2101 gcagcacgaa ctggcgcgcc aggcggcggt gattcggcgc cacgccttcg ccgaagagca
     2161 cgaacgacgg ctcgccgttg ccctgcggca gatcgccgaa cagctgatcg taggtgcggt
     2221 tctccttgat gatgtagacg acgtgatcga cgagcgaagg atcgcccggg gcgagaggca
     2281 ccgccgctgg ctcgggtttg tgccaggccg gctgtgaggc ggtggcccag gcgccgcgct
     2341 ggccggcgcc gagcaggccg gccgcgccgg gcgccacgag cggcaggcgg ttgttctcgg
     2401 cgaccgccag ggtgtagttg acgagatcgc ggcc
//
LOCUS       DAOSIF010000466         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37558_length_2429_cov_4.136479, whole genome shotgun sequence.
ACCESSION   DAOSIF010000466 DAOSIF010000000
VERSION     DAOSIF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37558_length_2429_cov_4.136479"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PLT35_12615"
     CDS             complement(<1..822)
                     /locus_tag="PLT35_12615"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG30113.1"
                     /translation="MSPHSAARRAAAALAYRDFRVLWTGACISSIGTWMQKVAQSWLV
                     LTLTGSPFYLGLDAFLGEMPILLLVLVGGVVADRRDRRRILLVSQYVQMSTAFALAIL
                     VHTDVVRIWHVLALSCITGTAQAFGSPAYQSLMPMLVRRHDVPNAIALNSIQFNLARI
                     VGPLAAGVALAAFGSAACFAINGLSFLVVVASLLMLRVRIAPAAAVRSVGEELRGGLA
                     FVRRHAAIRAFMFLAFCTALLGSPVLTLLPVVVQRVFQQDAAGFSLHFRLGFSLFF"
     gene            complement(934..1335)
                     /locus_tag="PLT35_12620"
     CDS             complement(934..1335)
                     /locus_tag="PLT35_12620"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOG30114.1"
                     /translation="MESSIMAIRVVRLGTPRVPGEGLRLGTVRRPPRGVPKAEFASRN
                     FYDLWLPELAPSEALVKTAQSASTDAEWRRFAKRYRAEMSSPSAARLLDLLAAFSRRA
                     DFSVGCYCENEDRCHRSVLRALLIERGARLA"
     gene            complement(1547..2017)
                     /locus_tag="PLT35_12625"
     CDS             complement(1547..2017)
                     /locus_tag="PLT35_12625"
                     /inference="COORDINATES: protein motif:HMM:NF022968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3225 domain-containing protein"
                     /protein_id="HOG30115.1"
                     /translation="MTRAGMAPFVLAAALAAVFAGAAASEPPGDVEAGVRAVWDAQVA
                     AWNRGDLPGYMSAYWKSPDLAFFSNGTAFRGWQATLDRYRATYQSGDRRMGVLDFPEL
                     EVVVLGSDAALVRGRWRLKMSGGKEADGMTSVVFRRLPEGWRIVHDHSSSKTGG"
     gene            complement(2014..2301)
                     /locus_tag="PLT35_12630"
     CDS             complement(2014..2301)
                     /locus_tag="PLT35_12630"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG30116.1"
                     /translation="MKKRTDALTSIPGVGPAIAGNLRLIGIREVADLKRADPERLYRK
                     LERTLCEPIDRCALYVLRSAVYFASRTRHDPEKLKWWNWKDGAAKPQRRGR"
BASE COUNT          401 a          871 c          842 g          315 t
ORIGIN      
        1 aaaaaagagg gaaaatccga ggcggaaatg gaggctgaag cccgccgcat cctgctggaa
       61 cacgcgctgc accaccaccg ggaggagagt gagcaccggc gaaccgagga gcgccgtgca
      121 aaacgccagg aacatgaacg cgcggatggc ggcgtggcgc cgcacgaagg ccaggccgcc
      181 ccggagttcc tcgccgacgg atcgcacggc cgccgccggc gcgatccgca cgcggagcat
      241 gagcagcgac gcgacgacca cgaggaagga gaggccgttg atcgcgaagc acgcggcgga
      301 gccgaacgcc gccagggcga cgccggcggc cagcgggccg acgatacgcg ccaggttgaa
      361 ctggatcgag ttgagcgcga tggcgttggg gacgtcgtgg cggcgcacca gcatcggcat
      421 gagcgactgg tacgccgggc tcccgaaggc ctgggccgtg cccgtgatgc acgagagcgc
      481 cagcacgtgc cagatgcgga cgacgtccgt gtgcacgagg atcgccagag cgaacgccgt
      541 gctcatctgc acgtactggg acacgagcag gatccggcgc cggtcgcggc ggtcggcgac
      601 gacgccgccg acgaggacga gcagcaggat cggcatctcg cccaggaacg cgtcgagccc
      661 gaggtagaac ggcgacccgg tgagggtcag gaccagccag ctctgggcga ccttctgcat
      721 ccacgtgccg atgctcgaga tgcaggcgcc cgtccacagg acgcggaagt cgcgataagc
      781 gagggcggca gccgcgcgtc gggcggcgga gtggggagac acgctctgcc catcttagcc
      841 gggaccagcg gaccgtgcgc cgaccttcgc gcggaccggc acccgcagcc tcgtggctgc
      901 cgctcgcccc cgtccggcgc gaatcgtcgc gggctacgcg aggcgcgccc cgcgctcgat
      961 gagcagcgcc ctgaggacgg agcggtgaca gcggtcctcg ttctcgcagt agcagccgac
     1021 cgagaagtca gcgcgtctgg agaacgccgc cagcaggtcc agcaggcgag cggctgacgg
     1081 gctgctcatc tcggcccggt accgcttcgc gaagcgccgc cactcggcgt cggtcgaagc
     1141 agactgcgcc gttttcacga gcgcctcgga cggggccagt tcgggcagcc agaggtcgta
     1201 aaagttccgc gaggcgaact ccgccttcgg cacgccgcgc ggcggccgcc ggacggtgcc
     1261 caggcgcagc ccttctcccg gaactctcgg cgtgccaagt cgaacgacgc ggatcgccat
     1321 gatgctgctc tccattccgc ccgcttcccg tcagcgcgag gcgggcgcga cgcacccgcg
     1381 accgacggaa ggccggcttc gttcgtctcc cggaccacat tcctgccagc gcgctgtcgc
     1441 cagcccgcgt ggcgaccaag accccgcacc gcccgcaggc gagcccgcag ccgcgagtcg
     1501 cggagcctcc ctccgggcgg cttcccggcc tcgcggcatg cggcgatcag ccgcctgtct
     1561 tcgacgacga gtggtcgtgg acgatgcgcc agccttccgg cagcctccgg aacacaaccg
     1621 acgtcatgcc gtcggcttct ttcccgccgg acatcttcaa ccgccaccgg ccgcgaacca
     1681 gcgccgcgtc cgatccgagc acgacgactt ccagctccgg gaaatcgagg acgcccatcc
     1741 gccgatcgcc gctctggtag gtggcccggt agcgatcgag cgtggcctgc catccgcgga
     1801 aggcagtccc gttcgagaag aacgccaggt ccggcgactt ccagtaagcg ctcatgtaac
     1861 ccggcaggtc gccccggttc caggctgcga cctgcgcgtc ccacacggcg cgcacgccgg
     1921 cctccacatc acccggcggc tctgatgcgg ccgcgccggc gaacacggcg gcgagggcgg
     1981 ccgcgagcac gaatggcgcc atccccgcgc gcgtcaccgg cccctccgct gcggcttcgc
     2041 ggcgccgtcc ttccagttcc accacttgag tttctcagga tcgtgcctcg tccggctggc
     2101 gaagtacacc gccgagcgga ggacgtacag agcgcagcga tcgattggct cgcagagcgt
     2161 gcgctcgagc ttccggtaca ggcgttccgg gtccgcgcgc ttcagatcgg cgacctcgcg
     2221 gatgccgatc aggcgaaggt ttccggcaat ggccgggccg acgccgggga tcgaggtgag
     2281 ggcgtccgtc cgcttcttca tctcgggcct ctgcctccga tcacgtcagg gaatgcaggc
     2341 cggtgacgtt gctctcggtg tcgcccgcga gcgcgacgaa tccgtgctcg ccgatcgagc
     2401 actcctcctt gacgatcgcg cttccggcc
//
LOCUS       DAOSIF010000467         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37584_length_2428_cov_3.905605, whole genome shotgun sequence.
ACCESSION   DAOSIF010000467 DAOSIF010000000
VERSION     DAOSIF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37584_length_2428_cov_3.905605"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PLT35_12635"
     CDS             <1..1041
                     /locus_tag="PLT35_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOG30117.1"
                     /translation="RACFGWMPGGTAVVTAALCAFFTVFTGGSGVTILALGGLLLPAL
                     LADKYRESFSLGLLTASGSLGLLFPPALPLILYGIVAGVAIGDLFLGGLLPGLVMLTF
                     LALLGVREGIVSGSGRTPFHLRAAVSAVWRAKWELLMPIVILGSLFAGATTVQSAALV
                     ALYTLVVQRVVQRELTSFGDLRRVVSAAIAVIGGVLVILAVAVGFTTYLIDAQVPARM
                     VDWVQANIHSAALFLLALNVFLIIVGCLMDIFSAIVVVVPLIVPIARVYGIDPVHLGV
                     IFIANLELGYLTPPVGLNLFLSSYRFNKPVLQIARASLPMLGVLAAGVLLITYVPWLT
                     TGLLDLLGRTQP"
     gene            1141..>2428
                     /locus_tag="PLT35_12640"
     CDS             1141..>2428
                     /locus_tag="PLT35_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOG30118.1"
                     /translation="MRSRSFAAIFVVCALVVGAAVASVAQRGPAPPVWPGSLGKGVTL
                     LPNGWKIQPAGRHIGVGDLPLAMAESPDGRFLVITNNGYAKPTLTVVDLRTGEVSSRV
                     TLDHAWLGLAWHPDGRRLFSSGAGQTTVNEFYWASNRLTPGAVYALGRDTQRPMPGIN
                     RPEPVEQSFVGGVSLSPDGRYLYAVHVLGEALTMLDLKTGLVRQTVDLGAEPYTCLVS
                     PDGSLVYVSVWGGARVAAFDAATLDKRAEIAVGEHPNAMVWSKDAARLFVACANTNSV
                     WVIDAAAKAATEQISIALFPNAPPGATPNALGLSPDGARLLVANADNNAVAVVDVSNA
                     ARSIVEGFIPTGWYPTAAQFSKDGSQIYVLSGKGLTSLPNPRGPQPGVPSSDGQYSGS
                     MLQGSLSVLPVPDAAALAALTRTVYALTPMTSAGILA"
BASE COUNT          284 a          891 c          848 g          405 t
ORIGIN      
        1 cgcgcctgtt tcggctggat gcccggcggc acggccgtcg tgacggcggc gctgtgcgcc
       61 ttcttcaccg tcttcaccgg cggatcgggc gtgacgatcc tcgccctggg cggcctgctg
      121 ctgccggcgc tgctggcgga caagtaccgc gagtcgtttt ctctcggcct gctgacggcc
      181 tcgggctcgc tcggcctgct cttcccgccg gccctgccgc tgatcctgta cggcatcgtg
      241 gccggcgtgg ccatcggcga tctcttcctg ggcggcctgc tgcccgggct ggtcatgctg
      301 acgttcctcg cgctgctcgg cgtgcgcgag ggcatcgtct ccggctccgg acggacgccg
      361 ttccacctcc gtgccgcggt gagcgccgtc tggcgcgcga agtgggagct gctcatgccg
      421 atcgtgatcc tcggctcgct cttcgccggg gccacgacgg tgcagtcggc cgcgctcgtc
      481 gcgctctaca cgctcgtggt gcagcgcgtc gtgcagcggg agctgacgtc gttcggggac
      541 ctgcgccgcg tcgtcagcgc cgcgatcgcc gtcatcggcg gcgtcctggt gatcctggcg
      601 gtcgccgtcg gcttcaccac ctacctgatc gacgcgcagg tgccggcgcg gatggtggac
      661 tgggtgcagg ccaacatcca ctcggcggcg ctgttcctgc tcgcgctcaa tgtcttcctg
      721 atcatcgtcg gctgcctgat ggacatcttc tccgccatcg tggtggtcgt gccgctcatc
      781 gtgcccatcg cccgggtcta cgggatcgat cccgtccacc tcggcgtcat cttcatcgcg
      841 aacctcgagc tggggtacct cacgccgccg gtcgggctga acctgttcct gtcgtcctac
      901 cggttcaaca agcccgtgct gcagatcgcg cgcgcctcgc tgccgatgct cggcgtgctc
      961 gcggcgggcg tgctgctcat cacctacgtg ccctggctga cgaccgggct gctcgacctg
     1021 ctggggcgca cgcagcccta gcgcccgcct gcgccgacgg gcctgccggc cggcgtcggc
     1081 ggcggcgctg cgctcgatcg ccgctggccc cgggccccgg accccgggta agattcgccc
     1141 atgcgatccc gatccttcgc cgccatcttc gtcgtctgcg cgctcgtcgt cggcgccgcc
     1201 gtggcgtcgg tcgcccagcg gggccccgcc ccgccggtct ggccgggcag cctgggcaag
     1261 ggcgtcacgc tgctccccaa cggctggaag atccagccgg cgggccgcca catcggcgtc
     1321 ggggatcttc cgctggccat ggcggaatcg cccgacggcc gcttcctcgt catcacgaac
     1381 aacggctacg ccaagccgac gctgacggtg gtggacctgc ggaccgggga ggtgagctcc
     1441 cgggtcacgc tcgaccatgc gtggctgggt ctggcgtggc acccggacgg gcggcggctc
     1501 ttctcgtcgg gggccgggca gaccacggtg aacgagttct actgggcgtc gaaccgcttg
     1561 acgccgggcg ccgtgtacgc gctcggccgc gacacgcagc gcccgatgcc aggcatcaac
     1621 cgccccgagc ccgtcgagca gagcttcgtc ggcggcgtat cgctctcgcc cgacgggcgg
     1681 tacctctacg cggtgcacgt cctcggagag gccctgacga tgctcgacct gaagacgggg
     1741 ctggtccgcc agaccgtcga tctcggggcc gagccgtaca cctgcctcgt gtcgcccgac
     1801 ggatcgctgg tctacgtgtc ggtctggggc ggggccaggg tggcggcctt cgacgccgcc
     1861 acgctcgaca agcgggccga gatcgccgtg ggtgagcacc cgaacgccat ggtgtggtcg
     1921 aaggacgccg cgcgcctgtt cgtcgcgtgc gccaacacaa acagcgtctg ggtcatcgac
     1981 gcggcggcga aggcggcgac ggagcagatc tcgatcgcgc tcttcccgaa cgcgccgccg
     2041 ggggcgacgc cgaacgcgct ggggctgtcg cccgacggcg cccgcctgct ggtcgccaac
     2101 gcggacaaca acgccgtggc ggtggtcgac gtcagcaacg ccgcgcggag catcgtcgaa
     2161 gggttcatcc cgacgggctg gtatccgacg gccgcgcagt tcagcaagga cggatcccag
     2221 atctacgtgc tgagcggcaa aggcctcacg tcgctgccca atccccgcgg cccgcagccc
     2281 ggcgtgccga gttcggacgg ccagtacagc gggtcgatgc tgcagggctc gctctcggtg
     2341 ctgcccgtgc ccgacgccgc cgccctggcg gcgctgacca ggacggtcta cgcgctcacg
     2401 cccatgacga gcgcgggcat cctggctc
//
LOCUS       DAOSIF010000468         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37702_length_2423_cov_2.979307, whole genome shotgun sequence.
ACCESSION   DAOSIF010000468 DAOSIF010000000
VERSION     DAOSIF010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37702_length_2423_cov_2.979307"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1537
                     /locus_tag="PLT35_12645"
     CDS             164..1537
                     /locus_tag="PLT35_12645"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG30119.1"
                     /translation="MRVDRTRLPVPGDERPFAFPRAVKRRLDNGLRLWSIERRGLPLV
                     SLVLVVPAGSAADPPDAPGLAALTADMLDEGSGVRSAVEIHEALSRLGAELDTAIGPD
                     AVELSLTVLDRFVPDALELLSDIVARPRLAEADLERVRALRAHRLQQLRSVPAACAEA
                     VFLRAVFGDHAYGHWPSGSSAAVSAINRQDVAAFHAAHYDPARATLAAVGAAPADALA
                     LAVEQTLGSWRAGAVESRAPSAVPPPHPGTGAARVLVVDRPGSPQTELRVGHVGAARK
                     DAAYYALVMLNAVLGGHFMSRLNSNLRERRGFTYGVRSSFDFRVAPGPFAVQTGVQTA
                     ATGQAAAEILEEMDALRGARPADERELALAAATLTNGYPMSFETIGQVARGLGQLALF
                     DLPDDTFERFTPAIRAVGTAEVARAACAHFRPGAATVVAVGDCERIGAGLGRLGLGDA
                     LIATAEF"
     gene            1665..2012
                     /locus_tag="PLT35_12650"
     CDS             1665..2012
                     /locus_tag="PLT35_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30120.1"
                     /translation="MTEPAATPGPASSNRGVMIVLSYLWILALVPLLTEKEDKEVQWH
                     AKHGIVLMVAEMAFWILYTILVNIIPLGCLMGALSPLVGLVFFIVHIAAIIKGLNGQR
                     LMIPYLSEYASKF"
     gene            2019..2321
                     /gene="yidD"
                     /locus_tag="PLT35_12655"
     CDS             2019..2321
                     /gene="yidD"
                     /locus_tag="PLT35_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG30121.1"
                     /translation="MRWLARTVLLGAACAFALDASRPPARQVSAGAAVAGIRFYQTRL
                     APHAGPWTAVCRFTPTCSRYAERVIARDGILRGGWLAVKRIARCGPWTPAGTHDPP"
BASE COUNT          291 a          897 c          867 g          368 t
ORIGIN      
        1 tcttcgagcg ggacctcgat cgataccgtc acgcctgccg ctcggccatc cgccgcgtgg
       61 cggccgccgt cctcgatccc gcgtgccgcg tgaccctgag cgtcgtgcct gccggcgccc
      121 ggccgctcgc ggcgcgggac tcgcagccgg cggcggtgtc gtgatgcgcg tcgatcgcac
      181 ccggctgccc gtccccggcg acgagcggcc cttcgccttc ccccgcgccg tcaagcggcg
      241 cctggacaac ggcctgcggc tgtggtcgat cgagcggcgc ggcctgccgc tcgtgagcct
      301 ggtgctggtc gttccggccg gcagcgcggc cgacccgccg gacgccccgg ggctcgctgc
      361 gctcacggcc gacatgctcg acgaaggctc gggcgtgcgc tcggccgtcg agatccacga
      421 ggcgctgtcg cgcctcggcg ccgaactcga caccgccatc ggcccagacg ccgtcgagct
      481 gtcgctcacg gtcctcgatc gcttcgtgcc ggacgccctg gagctgctct cggacatcgt
      541 ggcgcggccg cggctggccg aggcggatct cgagcgcgtg cgcgcgctgc gcgcgcaccg
      601 cctccagcag ctgcggagcg tcccggcggc gtgcgcggag gcggtgttcc tgcgcgccgt
      661 cttcggcgat cacgcctacg gacactggcc gtcgggatcc agtgcggcgg tgtccgcgat
      721 caaccggcag gacgtcgccg cgttccacgc tgcgcactac gatcccgcac gcgcgacgct
      781 cgccgccgtc ggggccgcgc cggccgacgc gctggccctg gccgtcgagc agacgctcgg
      841 aagctggcgc gccggcgccg tcgagagccg cgccccgtcc gccgtcccgc ccccgcaccc
      901 cgggaccggc gcagccaggg tgctggtcgt tgatcggccc ggctcgccgc agaccgaact
      961 gcgcgtaggc cacgtcggcg ccgcgcgcaa ggacgccgcg tactacgcgc tggtgatgct
     1021 caatgccgtc ctcggcggcc atttcatgag ccggctcaac tcgaacctcc gggagcgccg
     1081 gggcttcacc tacggtgtcc gttcgagctt cgacttccgc gtggcgcccg gcccgttcgc
     1141 cgtgcagacc ggcgtgcaga ctgcggcgac cggccaggct gccgcagaga tcctggagga
     1201 gatggacgcc ctgagaggcg cgcgccctgc cgacgagcgc gagctggcgc tggcggcggc
     1261 gacgctgacg aatggttatc ccatgagctt cgagaccatc gggcaggtgg cgcgaggcct
     1321 cgggcagctg gcgctgttcg acctgccgga cgacaccttc gagcgcttca cacccgccat
     1381 cagggctgtc ggtactgccg aggtcgcccg cgcggcctgc gcgcatttcc ggccgggcgc
     1441 cgcgacggtc gtggccgtcg gcgactgcga gcgcatcgga gccgggctcg gccgcctcgg
     1501 gctcggcgac gcgctcatcg ccacggccga gttctagccc ggcgccatgc gcgcgccggc
     1561 cgcgctggtt ctcctggcgg cccgttcgct ggatcttccg gtgtcggttc ggatacactc
     1621 cagccaacgt tcgatcgggt actcgcatat aaggagtcac acccatgacc gaacccgccg
     1681 ccacacccgg acccgcctcg agcaatcgcg gcgtgatgat cgtgctgtcc tacctctgga
     1741 tcctcgcgct ggttcccctg ctgacggaga aggaggacaa ggaggtccag tggcacgcca
     1801 agcacggcat cgtgctgatg gtggccgaga tggcgttctg gatcctgtac acgatcctcg
     1861 tgaacatcat cccgctcggc tgcctgatgg gagcgctgag cccgctggtg ggactggtgt
     1921 ttttcatcgt gcacatcgcg gcgatcatca agggcctgaa cggccagcgg ctgatgattc
     1981 cgtacctgtc ggagtacgcc agcaagttct aggcgcccgt gcgctggctg gcgaggacgg
     2041 tcctgctcgg cgcggcgtgc gcgttcgcgc tcgacgcgtc gcgcccgccg gcccggcagg
     2101 tttctgccgg cgccgcggtc gcggggattc gtttctacca gacccggctc gcgccgcacg
     2161 cggggccatg gacggcggtg tgccgcttca cgcccacctg cagccggtat gcggagcgcg
     2221 tcatcgcgag agacggcatc ctccggggag gctggctggc cgtaaagcgc atcgcgcgct
     2281 gcgggccgtg gacgccagcg ggcacgcacg atcctccctg acgatgcgcg gcggcgtcag
     2341 ccgggcagtc ccgcgccgcc ctgcggggcg cccggcgtcg agagaggcgc gtcgggctcg
     2401 aacatccggc gggtctcgtt ggt
//
LOCUS       DAOSIF010000469         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37743_length_2421_cov_3.533390, whole genome shotgun sequence.
ACCESSION   DAOSIF010000469 DAOSIF010000000
VERSION     DAOSIF010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37743_length_2421_cov_3.533390"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLT35_12660"
     CDS             complement(<1..1318)
                     /locus_tag="PLT35_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOG30122.1"
                     /translation="MDDHPLQRRLVLLMIWRLVIITLLLGSAAAIELGGAAAARPGPL
                     YALIAWTYGLSACYAVGLRLRRGAGWLAETQLALDALTAGAFVWMTGGAASYFSFLFV
                     LPILAASVLRSKPGVLRVTALSMLLYAGTVAVQYAGGAVQPWSVWAPDGPVALPQGLV
                     AQYTVAVHLVGLLAVGLLGGSLAERIRRADERLAVASAAMADLKAYSDDIIESLAMGL
                     VTTDAEGRVLTFNRAAEAITGHARASAPGLAAAGVLALPPDFTASLAGGIAAGATRRA
                     DYKYRKADGTLIDIGLSATMLQTPRGPAGFLYTFQDVTTIRRLEREARVQQRLAAVGE
                     MAAGIAHEIRNPLASISGSMQVLRSELGLTGEQAQLMDIVLQESERLNQTITSFLAYA
                     RPQRFAITRVDLRSAVEDTALLLRNSVEAGAAHAVEVDVPSSPVWCE"
     gene            complement(1330..>2421)
                     /locus_tag="PLT35_12665"
     CDS             complement(1330..>2421)
                     /locus_tag="PLT35_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG30123.1"
                     /translation="IAPAKARAAAAVKAARANKKVPAKNLAIFTRQFSVMIDAGLPLV
                     QCLEILGNQEEHKDFAAKILQTRADVEGGAALAEAMRKHPGAFDALYTNMVAAGEAGG
                     ILDAILKRLAVYIEKNVKLIGQVKSAMTYPIAVITIAILVIMLILWKVIPTFAALFAG
                     LGAELPLPTRLVIAASNGVVRVGPFLVVGAIAAAQAFRQYYRTNNGRHVVDRALLRAP
                     VLGNILRKIAVARFTRTLSTLISSGVPILDGLEITAKTAGNAIVEDAILTTRKSIERG
                     ETIADPLRQTGVFPAMVSQMVGVGEATGALDTMLAKIADFYEEEVDTAVAGLLTLLEP
                     IMIAFLGVVVGGVVIAMYLPIFSLISKLA"
BASE COUNT          347 a          843 c          891 g          340 t
ORIGIN      
        1 cctcgcacca cactggcgag gacggcacgt ccacctcgac ggcgtgcgcc gcgcccgcct
       61 cgacgctgtt gcgcagcagg agcgccgtgt cctcgacggc cgagcgcagg tccacgcgcg
      121 tgatcgcgaa gcgctgggga cgtgcgtacg cgaggaagct ggtgatcgtc tggttgagcc
      181 gctccgactc ctgcaggacg atgtccatga gctgcgcctg ctctccggtg agccccagct
      241 cgctccggag gacctgcatc gagcccgaga tcgaggcgag cgggttccgg atctcgtggg
      301 cgatgcccgc ggccatctcg ccgacggccg ccagccgctg ctgcaccctc gcctcccgct
      361 cgaggcgccg gatcgtcgtc acgtcctgga aggtgtagag gaaccctgcc ggccccctcg
      421 gcgtctggag catcgtcgcg ctcaggccga tgtcgatgag cgtgccgtcc gccttccggt
      481 acttgtagtc cgcccggcgg gtggcgccgg cggcgatgcc gccggcgagc gaggccgtga
      541 agtccggcgg cagggcgagc acgccggcgg ccgccaggcc gggggccgag gccctggcgt
      601 ggccggtaat cgcctcggcc gcgcggttga aggtgaggac ccgcccctcg gcgtcggtcg
      661 tcacgaggcc catcgcgagg ctctcgatga tgtcgtcgct gtaggccttg aggtccgcca
      721 tggccgccga cgcgaccgcc agccgctcgt ctgcgcggcg gatccgctcg gccagcgatc
      781 cgccgagcag gcccacggcc aggagcccga ccaggtgcac cgcgaccgtg tactgggcga
      841 ccaggccctg gggcagcgcg acgggcccgt ccggcgccca cacgctccac ggctgcacgg
      901 cgccgccggc gtactggacg gcgacggtgc cggcgtagag cagcatgctg agggccgtca
      961 cgcgcaggac gccgggcttc gagcgcagca cgctcgccgc caggatcggc aggacgaaca
     1021 ggaacgagaa gtagctcgcg gcgccgccgg tcatccacac gaaggcgccg gccgtcagcg
     1081 cgtccagggc gagctgcgtc tcggcgagcc agcccgcgcc gcggcgcagg cgcaggccga
     1141 ctgcgtagca ggcggagagg ccgtaggtcc acgcgatgag cgcgtacagg ggccccgggc
     1201 gcgcggccgc cgccccgccg agttcgatgg ccgccgccga gcccagcagg agcgtgatga
     1261 tgaccagccg ccagatcatc aggagcacga ggcgccgctg gagcgggtga tcgtccacgt
     1321 cccggcccct caggcgagct tgctgatcag cgagaagatc ggcaggtaca tcgcgatgac
     1381 gacgccgccg acgaccacgc cgaggaaggc gatcatgatc ggctcgagga gcgtcaggag
     1441 gcccgccacg gccgtgtcca cctcctcctc gtagaagtcg gcgatcttgg cgagcatcgt
     1501 gtcgagggcg cccgtcgcct cgccgacgcc gaccatctgg ctgaccatcg ccgggaagac
     1561 gcccgtctgc ctgaggggat cggcgatcgt ctcgccccgc tcgatgctct tgcgcgtggt
     1621 caggatggcg tcctcgacaa tcgcgttgcc ggccgtcttg gcggtgatct cgaggccgtc
     1681 caggatcggc acgcccgagc tgatgagcgt ggagagcgtg cgcgtgaagc gggccacggc
     1741 gatcttgcgg aggatgttgc cgagcaccgg cgcgcggagc agcgcgcggt ccaccacgtg
     1801 ccgcccgttg ttggtccggt agtactggcg gaaggcctgc gccgccgcga tggcgccgac
     1861 gacgaggaac ggcccgacgc gaaccacgcc gttgctggcc gcgatcacga ggcgcgtcgg
     1921 gagcggcagc tcggccccga ggcccgcgaa cagggcggcg aaggtcggga tgaccttcca
     1981 gaggatcagc atgatgacga ggatcgcgat cgtgatgacc gcgatggggt acgtcatcgc
     2041 cgacttgacc tggccgatga gcttgacgtt cttctcgatg tagaccgcca ggcgcttcag
     2101 gatggcgtcg aggatgccgc cggcctcgcc ggccgccacc atgttggtgt agagggcgtc
     2161 gaaggcgccc gggtgcttgc gcatggcctc ggccagggcg gccccgccct cgacgtcggc
     2221 ccgggtctgc aggatcttcg ccgcgaagtc cttgtgctcc tcctggttcc cgaggatctc
     2281 gaggcactgc acgagcggca gcccggcgtc gatcatgacc gagaactgcc gggtgaagat
     2341 ggcgaggttt ttcgccggca ccttcttgtt ggcgcgggcg gccttcaccg cggctgcggc
     2401 cctggccttc gcgggcgcga t
//
LOCUS       DAOSIF010000470         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_37746_length_2421_cov_3.312764, whole genome shotgun sequence.
ACCESSION   DAOSIF010000470 DAOSIF010000000
VERSION     DAOSIF010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37746_length_2421_cov_3.312764"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PLT35_12670"
     CDS             <1..1312
                     /locus_tag="PLT35_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOG30124.1"
                     /translation="ENVQRTYALSVHGKAVARGVRDAPVCTDCHGEHSIASASAPSSR
                     VSAANLPGTCAQCHAATRIITQYGLPPDTMSSYTQSFHGLALQGGNPTAADCASCHGA
                     HDILPSSDPQSKVNPARLPQTCGRCHPGIGNRLGAEFFRVHAPAGRHAENPWLVNLVT
                     LLYIIVIVVTIGGMALFVTLDYVKKARDHTRAVNADPFAEARLTPWLRHQHTILMVLF
                     IGLVYTGFVHRYPEAFFSWPFKAFSGGSEARALLHRIFGWSFTVLFVVHVAALLGTPG
                     GRQHARALWFGRVDFKDAVGVVAYNLGLRQDRPPARRWNYAEKAEYWALVWGSAVMTI
                     TGIMLVFSEALVRMWPKIVQDLAKVFHFYEAVLATLAIAVWHAYWTVFDPHEYPMNPA
                     WLIGKKAGHAARHAAGPPEAPALPGPETETATEPDAEPVVSSPE"
     gene            1358..2023
                     /locus_tag="PLT35_12675"
     CDS             1358..2023
                     /locus_tag="PLT35_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOG30125.1"
                     /translation="MTSGANPAAAPTDDLARTLWDYMLLRHDLAPADVIIALGSNDVR
                     VGEHAARLYLRGLAPLLLCSGGLGRLTVREFERSEAETFADAALRLGVPRAAVLVEAA
                     SANTGENIDFSRALLAERGIVPSRVIAVQKPYMERRAWATFKRRWPEPDLQVASPPIP
                     YDEYPTARIPRDLVISILVGDVQRMWVYAERGFQVPQPVPDDVRAAWRELVARGYRSH
                     LVG"
     gene            complement(2043..>2421)
                     /locus_tag="PLT35_12680"
     CDS             complement(2043..>2421)
                     /locus_tag="PLT35_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30126.1"
                     /translation="PKCYGLLNPNVRIILKAECRGRAALVLFSPQPGNYNAIVPESLG
                     LKRHDEVKFTCPLCSRDLTSRRNKAMAEVAFSTPGGDSGRVVFSRTYGHHETYFVTKE
                     EVRSYGRHADRDGVSFWGEGPTG"
BASE COUNT          341 a          853 c          866 g          361 t
ORIGIN      
        1 cgagaacgtg cagcgcacct acgcgctcag cgtgcacggc aaggcggtgg cgagaggcgt
       61 gcgcgacgcg cccgtgtgca cggactgcca cggcgagcac agcatcgcgt cggcgtcggc
      121 gccctcgtcg cgcgtgtccg cggcgaacct gcccggcacg tgcgcgcagt gccacgcggc
      181 cacgcgcatc atcacgcagt acggcctgcc gcccgacacg atgagctcgt acacccagag
      241 cttccacggc ctggcgctgc agggcggcaa ccccacggcg gccgactgcg cgtcctgcca
      301 cggggcccac gacatccttc cctcgagcga tccccagtcg aaggtcaacc cggcgcggct
      361 gccccagacg tgcggccggt gccaccccgg gatcggcaac cgcctcggcg cggagttctt
      421 cagggtgcac gcgccggccg ggcgccacgc cgagaacccc tggctcgtga acctggtgac
      481 gctgctgtac atcatcgtca tcgtggtgac catcgggggc atggcgctct tcgtcacgct
      541 cgactacgtc aagaaggccc gcgatcacac gcgggccgtc aacgccgacc cgttcgcgga
      601 ggcgcgcctg acgccgtggc tgcgccacca gcacaccatc ctgatggtgc tgttcatcgg
      661 gctcgtgtac acgggcttcg tccaccgtta cccggaggcg ttcttcagct ggccgttcaa
      721 ggcgttctcg ggcgggagcg aggcgcgggc gctgctgcac cggatcttcg ggtggagctt
      781 cacggtgctg ttcgtggtcc acgtcgcggc gctcctcggc acgccggggg ggcggcagca
      841 cgcccgggcg ctttggttcg gccgggtgga cttcaaggac gccgtcggcg tcgtcgccta
      901 caacctgggc ctgcggcagg accgcccgcc ggcgcggcgc tggaactacg ccgagaaggc
      961 ggagtactgg gcgctcgtct ggggctcggc ggtgatgacg atcaccggca tcatgctcgt
     1021 cttcagcgaa gccctggtgc ggatgtggcc gaagatcgtg caggacctcg ccaaggtgtt
     1081 ccacttctac gaggccgttc tcgcgacgct cgcgattgcc gtgtggcacg cgtactggac
     1141 cgtcttcgat ccgcacgagt acccgatgaa ccccgcgtgg ctcatcggca agaaggccgg
     1201 gcacgccgcc cgtcacgccg ccggccctcc tgaggcgccc gccctgcccg ggccggaaac
     1261 ggaaacggcg acagagccgg atgcggagcc ggtggtctcg tcgccggagt gaggctcgcg
     1321 agcgcggagg cgcccgcgcg gccggcggag cctcgccgtg acgagcggcg cgaacccggc
     1381 cgccgccccg accgacgatc tggcccggac actgtgggac tacatgctcc tgcgccacga
     1441 cctggcgccg gccgacgtca tcatcgccct gggcagcaac gacgtccgcg tgggagagca
     1501 cgcggcgcgg ctctacctga gagggctggc gccgctcctc ctgtgttccg ggggcctggg
     1561 caggctgacc gtcagggagt tcgagagaag cgaggcggag acgttcgcgg acgccgcgtt
     1621 gcgcctgggc gtgccgcgag cggcggtgct cgtcgaggcc gcgtccgcga acacgggaga
     1681 gaacatcgac ttctcgcgag ccctgctcgc ggagcggggg atcgtcccgt cgcgcgtcat
     1741 cgcggtgcag aagccctaca tggagcggcg cgcgtgggcg acgttcaagc gtcggtggcc
     1801 cgagccggac ctgcaggtcg cctcgccgcc cattccgtac gacgagtatc ccaccgcgcg
     1861 gattccccgc gacctcgtca tcagcatcct ggtcggtgac gtacaacgga tgtgggtgta
     1921 cgccgagcgc ggcttccagg tgccgcagcc ggtgccggac gacgtgcggg ccgcctggcg
     1981 cgagctcgtc gcccgcggct acaggtcgca ccttgtcggc tgaccgcccg tgactggccg
     2041 gactagccgg tcgggccctc gccccagaag ctgacgccgt cccggtccgc gtggcggccg
     2101 tagctccgga cctcttcctt cgtcacgaag tacgtctcgt ggtgtccgta ggtacgcgag
     2161 aacaccacgc ggccgctgtc gccgccgggc gtcgagaagg cgacttcggc catcgccttg
     2221 ttgcgccgcg aggtgagatc ccgcgagcac agggggcacg tgaacttgac ctcgtcgtgc
     2281 cgcttcaggc cgagggactc cgggacgatg gcgttgtagt tgccaggctg ggggctgaag
     2341 aggacgaggg ccgcgcgccc gcggcactcc gccttcagga tgatcctgac gttcgggttg
     2401 aggagcccgt agcacttcgg g
//
LOCUS       DAOSIF010000471         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38007_length_2409_cov_3.703059, whole genome shotgun sequence.
ACCESSION   DAOSIF010000471 DAOSIF010000000
VERSION     DAOSIF010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38007_length_2409_cov_3.703059"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLT35_12685"
     CDS             <1..404
                     /locus_tag="PLT35_12685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG30127.1"
                     /translation="VVLLPLADDGRIVLIRQYRFALDQWIWELPAGSLDPGEDPVAAA
                     ARECEEEIGLVPSRVEHAGSWYPTPGFCTEVMHYYRLTGLRRPGPDRPQPHADPDEDI
                     RVHVFTLDEARDMVRRGDIVDLKTAWGLTLA"
     gene            571..2235
                     /locus_tag="PLT35_12690"
     CDS             571..2235
                     /locus_tag="PLT35_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOG30128.1"
                     /translation="MSDPTVPRHPKGTRARILLTSVFGPYAQDDEFGSRSINPMELYH
                     NQVTREQGPFSIRMFHRSWGILFIQHNISAPCALLDFPTRERFVAELQRGEYDVVGIS
                     SIIVNVGKVREMCRLVREHAPGAAIVVGGHVTAIPGLHRLIDADHIVRGEGIAWFRAF
                     LGEPAGAPIRHPAIPSAFGFHLMGLRAPQGGGSPAATIVPSVGCPMGCNFCTTSAFFG
                     GKGRVVNFFEKGQELFRVMCEAESRLKVKAFFIMDENFLLYKKRAMELLEQMRAKGKA
                     WQLYVFSSANAIAKYSMRELVELGISWVWMGLEAKQSRYSKLAGADTMALTKQLQAHG
                     IGVLGSTIVGLDHHTPDNIAGEIEHAVSHETDFHQFMLYTPVPGTPLYQEMADEGRLL
                     ADVDLADIHGQDRFNFRHAAIPREQSKRLLDWAFRLDYERNGPSLYRMARTMLEGWRR
                     YRDDPDPRVRQRARDCARDLRRGYGAALFAMEKYLRRSNAEVSARIGALRREMEREIG
                     GITRWVSRLAGPVLLWSARRDARRHPRGRRLEPATFFERRNWPTTA"
BASE COUNT          355 a          840 c          872 g          342 t
ORIGIN      
        1 ccgtcgtgct cctgcccctg gcagacgacg ggcgcatcgt cctgatccgc cagtaccggt
       61 tcgcgctcga ccagtggatc tgggagctgc ccgccggcag cctggatccg ggcgaggacc
      121 ccgtcgcggc ggccgcgcgc gagtgcgaag aagagatcgg cctcgtgccg tcgcgcgtcg
      181 agcacgccgg ctcctggtac ccgacgccgg gcttctgcac ggaggtcatg cactactacc
      241 ggctgaccgg ccttcgccgg cctggcccgg accgtccgca gccgcacgcg gaccccgacg
      301 aggacatccg cgtgcacgtc ttcacgctgg acgaggcgcg cgacatggtc cgccgtggcg
      361 acatcgtcga tctgaagacc gcctggggcc tgacgctggc ctgacgcccc tgccgaggcc
      421 ggcaggcgcg gcgggcgcgg cctgcaggac gcgacggctc cccgggctcg cggctccccg
      481 tgcgataatg cccgttcagg gacagcgagc cggccgcgat cgtcagtggc gctggcggcc
      541 cgcggcgatt cgagaaagga acgcgccccc atgtccgacc cgaccgtccc gcggcatccc
      601 aaaggcacgc gagcccggat cctccttacg tcggtgttcg gcccctacgc ccaggacgac
      661 gagttcggca gccggtcgat caacccgatg gagctgtacc acaaccaggt gacgcgggag
      721 caggggccgt tctcgatccg gatgttccac cggtcgtggg gcatcctgtt catccagcac
      781 aacatctcgg cgccgtgcgc gctgctcgac ttccccaccc gcgagcggtt cgtcgcagag
      841 ctgcagcggg gggagtacga cgtcgtcggc atttcgagca tcatcgtgaa cgtcggcaag
      901 gtgcgcgaga tgtgccggct cgtgcgcgag cacgcgccgg gcgccgcgat cgtcgtcggc
      961 gggcacgtga ccgccatccc cggcctgcat cgcctgatcg atgccgacca catcgtgagg
     1021 ggcgagggca tcgcctggtt ccgcgcgttc ctgggcgagc ccgcgggggc gccgatccgg
     1081 caccctgcca tcccgtcggc gttcggtttc cacctgatgg gcctgcgcgc gccgcagggc
     1141 ggcgggtcgc cggccgcgac gatcgtgccg tcagtgggct gccccatggg gtgcaacttc
     1201 tgcacgacct cggccttctt cggaggcaag ggccgcgtcg tcaacttctt cgagaagggg
     1261 caggagctct tccgggtgat gtgcgaggcc gagtcccggc tgaaggtgaa ggcgttcttc
     1321 atcatggacg agaacttcct gctctacaag aagcgggcga tggagctgct cgagcagatg
     1381 cgggcgaagg ggaaggcctg gcagctctac gtgttctcct cggcgaacgc aatcgcgaag
     1441 tactcgatgc gcgagctcgt cgagctgggc atcagctggg tgtggatggg ccttgaggcg
     1501 aagcagagcc ggtacagcaa gctggcgggc gccgacacga tggcgctcac gaagcagctg
     1561 caggcgcacg ggattggggt gctgggatcg acgattgtcg gcctcgacca ccacacgccg
     1621 gacaacatcg cgggcgagat cgagcacgcg gtgtcgcacg agacggactt ccaccagttc
     1681 atgctctaca cgccggtgcc gggcaccccg ctctaccagg agatggcgga cgagggccgg
     1741 ctcctcgcgg acgtggacct ggcggacatc cacggccagg accgcttcaa cttcaggcat
     1801 gcggcgatcc cgcgcgagca gtcgaagcgg ctcctcgact gggcgttccg gctcgactac
     1861 gagcgcaacg ggccgagcct ctaccgcatg gcgcggacga tgctggaggg ctggcggcgg
     1921 taccgggacg atcccgaccc gcgcgtgcgg cagcgggcgc gcgactgcgc ccgcgatctg
     1981 cggcgcgggt acggcgccgc gctgttcgcg atggagaagt acctgcgccg gtcgaacgcg
     2041 gaggtcagcg cccggatcgg cgcgctgcga agggagatgg agcgcgagat cggcggcatc
     2101 acgcggtggg tgtcgcgcct ggccggcccg gtgctgctgt ggtccgcgcg ccgcgacgcg
     2161 cgccggcacc cgaggggccg caggctcgag ccggcgacct tcttcgagcg ccgcaactgg
     2221 ccgacaaccg cctgaggcgg ccggcgctca cgcgccgagc ccgatggctc ctcaatcgtc
     2281 gcggcgtaga tggcgaccgt gtccgccggg tccctcgcgt agccgcccga cagcacgacc
     2341 gccaccggga tgccggcgtc gcgcgaggcg gcgaacgccg cggcgtcccg ccggcgcagg
     2401 ccgtccatc
//
LOCUS       DAOSIF010000472         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38055_length_2407_cov_3.473639, whole genome shotgun sequence.
ACCESSION   DAOSIF010000472 DAOSIF010000000
VERSION     DAOSIF010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38055_length_2407_cov_3.473639"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /gene="gndA"
                     /locus_tag="PLT35_12695"
     CDS             complement(<1..1239)
                     /gene="gndA"
                     /locus_tag="PLT35_12695"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR00873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOG30129.1"
                     /translation="MQCDIGVVGLGVMGANLARNMERNGFAVAGYDLEAAKGQAFLAG
                     PAGGTRAAVAPSPEALMAMLKRPRRVLLMVPAGAPVDSAIDHLKPHLEPGDILIDGGN
                     SWFLDTERRSKALASGGFHYIGAGVSGGEQGALWGPAIMPGGQREAWEALAPILRAIA
                     ARADDGEPCVDYMGPGGAGHYVKMVHNGIEYGDMQLIAEAYDLLSRGLGLPAAELAAV
                     FRAWNAGELRSYLVEITAEVLDAVDPETGAPLVDLILDEAQQKGTGKWTSQHAFDVGA
                     PVPTINAAVESRILSALKAERVRASRLLRGPSPRLRGSREALVDAARAALYASKITSY
                     AQGFGLLRMASAEYGYGLDLAGVARVWRAGCIIRASLLGDITAAFGRDAALQNLLLDE
                     AFADAVESRQEAWRLVVQTAV"
     gene            complement(1229..>2407)
                     /locus_tag="PLT35_12700"
     CDS             complement(1229..>2407)
                     /locus_tag="PLT35_12700"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOG30130.1"
                     /translation="VLALIPDGTRTMPMPLLFGILSASLGPRAAALDFLVALGTHQPM
                     SDAQLGRLLGRPVTAGSSHGHRIFNHRWDLPETFVTLGVIPAREIAELSGGRLERDVP
                     VTLNRLLLDYDHLLICGPVFPHEVVGFSGGNKYLVPGVAGREIIDVTHWLGALLTSSA
                     VIGAGYTPVRAVIDRAASFVKRPVTCISLVVTHAGVNGVFTGDPHESWEAASALSART
                     HITWVDRPFTRALSVMPEMYDDLWTAAKGMYKLEPAVADGGEVVIFAPHITEVSYTHG
                     RLIDEIGYHCLDYFLGQWDRFSRYPGGVIAHATHVKGLGRYDAATGIETPRIQVTLAT
                     GIPEERCRRINLGYLDPAAVDIGAWTGREAEGVLLVPRAGEMLYRLSEAAPGGATRAV
                     "
BASE COUNT          335 a          873 c          872 g          327 t
ORIGIN      
        1 gaccgccgtc tgcacgacga ggcgccaggc ttcctgcctg ctctccaccg cgtccgcgaa
       61 cgcctcgtcc aggaggaggt tctggagcgc cgcgtcgcgc ccgaacgccg cggtgatgtc
      121 gcccagcagc gacgcgcgga tgatgcagcc cgcccgccag acgcgcgcga cgcccgccag
      181 gtcgaggccg tagccgtact cggcggacgc catcctcagc aggccgaagc cctgcgcgta
      241 cgacgtgatc ttgctcgcgt acagggcggc gcgcgcggcg tcgacgaggg cctcgcggct
      301 gccgcggagg cggggggagg ggccgcgcag gaggcggctc gcccgcacgc gctcggcctt
      361 cagcgccgac aggatccggc tctcgacggc cgcgttgatc gtcgggacgg gcgcgccgac
      421 gtcgaacgcg tgctggctcg tccacttgcc ggtgcccttc tgctgggcct cgtcgaggat
      481 gaggtccacc aggggcgcgc ccgtctccgg gtcgacggcg tcgaggacct cggcggtgat
      541 ctccacgagg taggaccgca gctcgccggc gttccacgcc cggaacacgg ccgccagctc
      601 cgccgccggc aggccgaggc cgcgcgacag caggtcgtac gcctcggcga tgagctgcat
      661 gtcgccgtac tcgatcccgt tgtggaccat cttgacgtag tggccggcgc cgccgggccc
      721 catgtagtcc acgcacggct cgccgtcgtc ggccctggcg gcgatggcgc gcaggatggg
      781 cgcgagggct tcccaggcct cgcgctggcc gccgggcatg atggccgggc cccacagcgc
      841 gccctgctcg ccgccagaca cgccggctcc gatgtagtgg aacccgccgg aggcaagggc
      901 cttgctccgc cgttcggtgt cgaggaacca ggagttgccg ccgtcgatca ggatatcgcc
      961 cggctcgagg tgcggcttca ggtggtcgat ggcgctgtcg acgggcgcgc cggcgggcac
     1021 catcaggagc acgcggcgcg gcctcttcag catcgccatc agcgcctccg gcgagggcgc
     1081 gacggcggcg cgcgttccgc cggccgggcc tgccaggaac gcctggccct tggccgcctc
     1141 gagatcgtat cccgcgacgg cgaacccgtt gcgctccatg ttgcgcgcga ggttcgcgcc
     1201 catcacgccg agcccgacga cgccgatgtc acactgcacg ggtcgctcct cccggggccg
     1261 cctcggacag gcggtagagc atctcgccgg cgcgaggcac cagcagcacg ccttcggcct
     1321 cccgcccggt ccaggcgccg atgtccaccg ccgcggggtc caggtacccc aggttgatcc
     1381 gccggcaccg ttcttcaggg atcccggtgg ccagcgtcac ctggatccgc ggcgtctcga
     1441 tccccgtggc cgcgtcgtac cggccgaggc ccttcacgtg cgtcgcgtgc gcgatgacgc
     1501 cgccgggata gcgggagaag cggtcccact ggcccaggaa gtaatcgagg cagtggtagc
     1561 cgatctcgtc gatgaggcgg ccgtgcgtgt agctcacctc ggtgatgtgc ggcgcgaaga
     1621 tcaccacctc gccgccgtcc gccaccgccg gctcgagctt gtacatgccc ttggccgccg
     1681 tccacagatc gtcgtacatc tccggcatga ccgagagcgc gcgcgtgaac ggccggtcca
     1741 cccacgtgat gtgcgtgcgg gccgagagcg ccgacgcggc ttcccacgac tcgtgcggat
     1801 cgccggtgaa cacgccgttg acgcccgcgt gcgtcacgac gaggctgatg cacgtgacgg
     1861 gccgcttgac gaacgaggcg gcgcggtcga tgacggcccg gacgggcgtg tagcccgcgc
     1921 cgatgacggc gctgctcgtg agcagggcgc cgagccagtg cgtgacgtcg atgatctccc
     1981 gcccggcgac gcccggcacg aggtacttgt ttccgcccga gaaccccacg acttcgtgcg
     2041 ggaacaccgg gccgcagatg aggaggtgat cgtagtcgag cagcagacgg ttcagcgtga
     2101 ccgggacgtc ccgttcgagg cggccgcccg acagctccgc gatctcgcgg gccgggatca
     2161 cccccagcgt gacgaacgtc tccggcaggt cccagcggtg gttgaagatc cgatgcccgt
     2221 gcgaggaccc ggcggtcacc ggccgcccga ggagacgccc gagctgcgcg tcggacatcg
     2281 gctggtgcgt gccgagggcc accaggaagt ccagcgccgc cgcccgcggg ccgaggctcg
     2341 cggagaggat gccgaacagg agcggcatcg gcatcgtgcg cgtgccgtca ggaatgagcg
     2401 ccaggac
//
LOCUS       DAOSIF010000473         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38073_length_2406_cov_3.208422, whole genome shotgun sequence.
ACCESSION   DAOSIF010000473 DAOSIF010000000
VERSION     DAOSIF010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38073_length_2406_cov_3.208422"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLT35_12705"
     CDS             complement(<1..412)
                     /locus_tag="PLT35_12705"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOG30131.1"
                     /translation="MTLFFAAVALLVAGYFTYGAIVDRVFGPDLGRTAPAVAQADGID
                     FVAMSWPRVFLIQLLNIAGVGPIFGALAGALWGPVAFLWIVFGSILAGGVHDYLSGLL
                     SMRNRGGSISEIVGFYLGPAMKDFARVFSVVLLVL"
     gene            complement(452..1042)
                     /locus_tag="PLT35_12710"
     CDS             complement(452..1042)
                     /locus_tag="PLT35_12710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG30132.1"
                     /translation="MIPGAILLAAAGWAFAFGPAWGNFWIKIGLAVAAVCAYALAFRK
                     PDLRVTAVSVAEGVVLAAVLYAVFFVGNALAPFIVPGARGQVGGIYALGEGSPRAWIF
                     LLLCFVTGPGEEIFWRGFLQEELQKRFTKAPGFLAATALYGAVHVFSGNLMLILAALV
                     AGAFWGALYAWRRDLVALIVSHSLWSAFIFAVAPVR"
     gene            complement(1039..1932)
                     /locus_tag="PLT35_12715"
     CDS             complement(1039..1932)
                     /locus_tag="PLT35_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOG30133.1"
                     /translation="MPTLKDYLVAARPWSFSMTAISVTLGTLVAWRSGPVEWGWYLLA
                     VAGTICCHAGGNVLNDYFDARNQVDQPDSATARYRPHPIVGGLMQPRTVLTEALFLFA
                     LAGAAGLLLALGRTWLVFWIALAGLLLAVFYSGGPITLKYRALGEAAVFAIWGPLMFL
                     GAYAVQRRALAWEPIVASIPFGVLVALVLYANNIRDIAQDKRSGIATLGTLLGQERAL
                     AGFTALMLAAYAYVAAAAALGAITPWTLLVLLSAPTAIGLLRTFRAGIPDASDAMTAR
                     LDTVFGLLYMAALALDRVVRL"
     gene            complement(2147..>2406)
                     /locus_tag="PLT35_12720"
     CDS             complement(2147..>2406)
                     /locus_tag="PLT35_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30134.1"
                     /translation="QFGGRRDVQLFVEELMAWHDEMVQHRRVVAQLGPDLACSDTCPH
                     QTGRRLWREAREILGPKADTLIFLRACATQPVAEEREREIA"
BASE COUNT          419 a          825 c          912 g          250 t
ORIGIN      
        1 cgagcacgag cagcaccacc gagaacacgc gagcgaagtc cttcatcgcc ggcccaaggt
       61 agaagccgac gatctccgag atgctcccgc cccggttgcg catcgagagc aggcccgaca
      121 ggtagtcgtg cacgccgccg gccaggatgc tgccgaacac gatccacagg aaggcgaccg
      181 gcccccacag cgcgccggcc agggcgccga aaatggggcc gacgcccgcg atgttcagca
      241 gctggatcag gaagaccctg ggccagctca tcgcgacgaa gtcgatgccg tcggcctgcg
      301 ccacagcggg cgccgtcctg ccgagatcgg gcccgaacac gcgatcgacg atggcgccgt
      361 aggtgaagta cccggcgacg agcagcgcga cggcggcgaa gaagagcgtc atggcagtcc
      421 ccgggccgag gctccgcagg ggtccggacc gtcagcgaac cggcgcgacg gcgaagatga
      481 acgcgctcca gagcgagtgc gagacgatga gcgccaccag gtcccgccgc catgcgtaga
      541 gcgcgcccca gaaggcgccg gcgacgaggg cggccaggat cagcatgagg ttgcccgaga
      601 acacgtgcac ggcgccgtag agggccgtcg ccgcgaggaa gcccggggct ttcgtgaaac
      661 gcttctgcag ctcctcctgc aggaacccgc gccagaagat ctcctcgccc ggcccggtga
      721 cgaagcagag cagcaggaag atccaggcgc gcggcgagcc ttcgccgagg gcgtagatgc
      781 cgccaacctg tccgcgcgcc ccgggcacga tgaagggagc cagggcgttg ccgacgaaga
      841 agacggcgta cagcaccgcc gccaggacga cgccctccgc gaccgacacc gcggtcacgc
      901 gcaggtcggg cttgcggaac gcgagggcgt aggcgcagac cgccgcgacc gcgaggccga
      961 tcttgatcca gaagttcccc cacgccgggc cgaacgcgaa cgcccagccg gcggcggcga
     1021 ggaggatggc gcccgggatc acagccgcac cacccggtcg agggccagcg cggccatgta
     1081 gagcaggccg aagaccgtgt ccagccgggc ggtcatggcg tcggaggcgt cgggaatgcc
     1141 cgcgcggaac gtccgcagga ggccgatggc cgttggcgcc gagagcagca cgagcagcgt
     1201 ccacggggtg atcgcgccga gggcggcggc ggcggcgacg taggcgtagg cggcgagcat
     1261 gagcgccgtg aagccggcga gagcgcgctc ctggccgagc agcgtcccga gcgtcgcgat
     1321 gccggaccgc ttgtcctggg cgatgtcgcg gatgttgttg gcgtagagga cgagggccac
     1381 cagcacgccg aacggaatgg aggcgacgat cggctcccag gcgagcgccc gccgctgcac
     1441 ggcgtacgcg ccgaggaaca tcagcggacc ccagatcgcg aacacggccg cctcgccgag
     1501 cgcgcggtac ttcagcgtga tcgggccgcc cgagtagaag acggccagca gcagcccggc
     1561 cagcgcgatc cagaacacca gccacgtccg gccgagggcg aggaggaggc cggcggcccc
     1621 ggccagcgcg aagaggaaga gggcctccgt cagcaccgtg cgcggctgca tcaggccccc
     1681 gacgatcggg tgcggccggt accgcgccgt tgcggagtcg ggctgatcga cctggttgcg
     1741 cgcgtcgaaa tagtcgttca gcacgttgcc cccggcgtgg cagcagatcg tgccggcgac
     1801 ggccaggagg taccagcccc actcgacggg gcctgaccgc caggcgacga gcgttccgag
     1861 cgtcacggaa atcgcggtca tgctgaacga ccacgggcgg gcggcgacga ggtagtcctt
     1921 cagggtcggc atcgctccag tcggtcctct cgtgcgggga agccccatcg taccggacgg
     1981 cgcgcggagg gacaagggcg cggagggacc agggcgcgga gggctctggg ggagcagggg
     2041 gcgccgggct tcgggccgga ggatggccgc ggatgggcgc acgaacgccc cgggcgccgg
     2101 ggggccggcg gcgcgcgcat cctggccgag cccgggcggg agcccgtcag gcgatttccc
     2161 gctcgcgctc ctctgccacg ggctgcgtcg cgcaggcgcg caggaagatc agcgtgtcgg
     2221 ccttgggccc gaggatctcg cgggcctcgc gccagaggcg ccggccggtc tggtgcgggc
     2281 acgtgtccga gcacgccagg tccggcccga gctgggcgac gacgcggcgg tgctgcacca
     2341 tctcgtcgtg ccaggccatc aattcctcga cgaacagctg gacgtcccgt cgtccgccga
     2401 actgcc
//
LOCUS       DAOSIF010000474         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38249_length_2397_cov_3.384714, whole genome shotgun sequence.
ACCESSION   DAOSIF010000474 DAOSIF010000000
VERSION     DAOSIF010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38249_length_2397_cov_3.384714"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLT35_12725"
     CDS             <1..1018
                     /locus_tag="PLT35_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30135.1"
                     /translation="LPGPPLDEGLDSGRFAHILVTEIQPFVKRRAGEVRSRLTNTTHL
                     VNTDVRMRILDAPQLFYEGFFLYHGPDAFGQRTDTLSNGLSLHHAFSRALSAYGRVAR
                     EQGREPRGRRTATVSNATITAEPFPTLRSSLLYTGLDEEIAGLPNTRRAVFIQNAAQP
                     YRGVDILFGFGWNATEWETGEIARDRLLNLSGTVAPRQHVSLTFSYDATTTRRSGTFA
                     GAPRARIRRSYGAVVVDPVRTLHVVLGGEVIAVSGQRTRTTLDIGANWAPFPDGKLQI
                     IFASNDALRALEFGRERSTLGAVRWNVSRRSYIDVSYQRTRSEFVVQTTESRMCSVTV
                     RTFF"
     gene            1038..1634
                     /locus_tag="PLT35_12730"
     CDS             1038..1634
                     /locus_tag="PLT35_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOG30136.1"
                     /translation="MRRAPGLVAFVLLGIVASASIGAAQVTDVYRDPRMDFGSIRTIA
                     VAPFANLARDQVVAERVRDVFVNRLLATGAVYVLPVGEVARGLAKLEIQNPTTPTPDD
                     VVKLGALLEAQAVITGVVREYGEVRSGTTSSNVISMSIQLVETGTGKIVWSASSTKGG
                     ISFWDRLIGGGGQPLNRVTEEAIDALFDKLLGPPQKGR"
     gene            1644..>2397
                     /locus_tag="PLT35_12735"
     CDS             1644..>2397
                     /locus_tag="PLT35_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30137.1"
                     /translation="MPRAVVGLVYLLFFLSGAAALVYQVVWVRSLTLVFGGSHLAVTA
                     VLSIFMAGLAAGGFLVGRRVDGVERPLRLYGFLELGIAASALAAAGLMRAYPSVYAAL
                     AQGRDDAPLYLTLLRIPFAAGALIVPTTLMGGTLPVLSRFVARQPQDLRGYLSFLYGF
                     NTLGSVLGAFLAGFVFLRLWAVSTTLYVAVAASALIGVISLALSGRLAGSPVPPSPGG
                     DGPAAPEPPLGRLPFTLVLWGIGVSGFCALGYE"
BASE COUNT          327 a          846 c          834 g          390 t
ORIGIN      
        1 cctccccggc ccgccgcttg acgaagggct ggactcgggc cggttcgccc acatcctcgt
       61 caccgagatc cagcccttcg tcaagcggcg ggccggggag gtgcgcagca ggctcacgaa
      121 cacgacgcac ctcgtgaaca ccgacgtccg gatgcggatc ctcgacgccc cgcagctctt
      181 ctacgagggc ttcttcctct accacggacc ggacgcgttc ggccagcgca ccgacacgct
      241 gtcgaacggg ctgtcgctgc accacgcctt cagccgcgcc ctctccgcct acgggcgcgt
      301 ggcccgtgag caggggcgag agccgcgggg ccggcggacc gccacggtga gcaacgcgac
      361 gatcacggcc gagccgttcc cgacgctgcg gagctcgctg ctgtacaccg gcctggacga
      421 ggagatcgcc gggctcccga acacccggcg cgcggtgttc atccagaacg cggcgcagcc
      481 ctaccgcggc gttgacatcc tgttcggctt cggctggaat gccaccgaat gggaaacggg
      541 cgagatcgcg cgcgaccggc tcctgaacct gagcggaacc gtggcgccgc ggcagcacgt
      601 gagcctgacg ttcagctacg acgccacgac cacgcggcga tccgggacgt ttgccggcgc
      661 gcctcgggcc cgcatccgcc ggtcctacgg cgccgttgtc gtcgatcccg tccggacgct
      721 ccacgtcgtc ctgggcgggg aagtcatcgc cgtcagcggc cagcggacga ggacgacgtt
      781 ggacatcggc gcgaactggg cgccgttccc tgacggcaag ctgcagatca tcttcgcctc
      841 gaacgacgcc ctgagggccc tggagttcgg cagggagcgc agcaccctgg gcgccgtgcg
      901 atggaacgtc tcgcggcggt cgtacatcga cgtctcctac cagcggacgc ggagcgagtt
      961 cgtcgtgcag acgaccgaat cgaggatgtg cagtgtgacg gtgaggacgt ttttctgatg
     1021 gattgtgagg cgacggcatg agaagagcgc ctggtcttgt ggctttcgtc ctgctgggga
     1081 tcgtggcctc agcgtcgatc ggcgcggcac aggtcaccga tgtctaccgg gacccgagaa
     1141 tggacttcgg cagcatccgc acgatcgccg tcgcgccgtt cgccaacctc gccagggacc
     1201 aagtcgtggc cgagcgggtc cgcgacgtct tcgtcaaccg cctcctggcc accggcgcgg
     1261 tgtacgtgct cccggtgggc gaggtggcgc gaggcctggc gaagctggag atccagaacc
     1321 cgaccacccc gacccccgac gacgtcgtca agctcggcgc gctgctcgag gcgcaggcgg
     1381 tcatcacggg ggtcgtccgc gagtacggcg aggtgcggtc ggggaccacc tcctcgaacg
     1441 tcatctcgat gagcatccag ctcgtcgaga ccgggacggg gaagatcgtg tggagcgcct
     1501 cgtccaccaa gggcggcatc agcttctggg accgcctgat tggagggggc ggccagccgt
     1561 tgaaccgggt caccgaggag gctatcgacg cgctcttcga caagctgctc ggcccgccgc
     1621 aaaagggccg gtgatccgcc gcgatgcctc gcgccgtcgt cggcctcgtc tacctgctgt
     1681 tcttcctgtc cggggcggcc gccctcgtgt accaggtggt ctgggtccgc tccctcacct
     1741 tggtgttcgg cgggtcgcac ctggccgtga cggcagtcct gtcgatcttc atggcggggc
     1801 tggcggccgg cgggttcctc gtcggccggc gggtggacgg cgtggagagg ccgctgcgcc
     1861 tctacggctt cctggaactc ggcatcgcgg catccgccct tgcggccgcc gggctgatga
     1921 gagcctaccc ctcggtctac gccgcgctgg cccaggggcg cgacgacgcg ccgctctacc
     1981 tcacgctgct ccggatcccc ttcgccgccg gcgcgctgat cgtgccgacg acgctgatgg
     2041 gcgggacgct gccggtcctc tcgcggttcg tcgccaggca gccgcaggac ctgcgcgggt
     2101 acctctcgtt tctgtacggc ttcaataccc tcgggtccgt gctcggagcg ttcctggcgg
     2161 gtttcgtctt cctgcgcctc tgggcggtca gcacaacgct ctacgtcgcc gtggcggcca
     2221 gtgcgctcat cggcgtcatc agcctcgcgc tgtcgggcag gctggccgga tccccggtgc
     2281 cgcccagccc cggcggggac gggccggccg cacccgagcc gccactcggg cggctgccat
     2341 tcacgctggt gctctggggc attggcgtca gcggcttctg cgccctgggc tacgagg
//
LOCUS       DAOSIF010000475         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38496_length_2383_cov_3.707904, whole genome shotgun sequence.
ACCESSION   DAOSIF010000475 DAOSIF010000000
VERSION     DAOSIF010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38496_length_2383_cov_3.707904"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2383
                     /locus_tag="PLT35_12740"
     CDS             <1..>2383
                     /locus_tag="PLT35_12740"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG30138.1"
                     /translation="LRLSARIWDSEATARAEVQSGQSTRVNFALRPDPNPDAGVIQGR
                     VIDRESNLPLAGAFVFFARLPINTVEPPAALNEFGAMMSIEPPAGGDDYVRTNGRGRY
                     QTPRLRPGVYVVIAWAPGFASQHVIVPLPVGGAAEANFALQPLEPMHPGVIEGRVIDR
                     LTGDPVAGAWVFYHPAVDDKAIAPHDAPDAAHPRVRTNARGEFWISPLRPGRYHLAVR
                     ADGFEPAHKNVNVEAGEVTAVRFLLEPKRPPHPGAIVGQVVDGETGDPVPGARVFFGR
                     PLDDDDTVEWWRKHDAATDHLNFVETDHQGFYRIKPLRPGAWHLLVRAAGYQPAARKV
                     VVRPGQTVEANFKLRPNDPRDPGSIVGRVTDGTSQTPLEGARVYYRPIPRDTVELDER
                     SPDSRFHPSVIVDSERPPFVETDANGRFKIEHLRPGRYQLLAVAAGYLPASEIVAVEQ
                     GHTTEASFALRPRPAGQGDLAGIVVDAQTHDPLRGAHVWVIPAGNVVIAEAMIPAVIL
                     GEAITDNTGRYRIEDLPTGPVIVFAAREGYRTARKRVEIPAGGEAVARFALQPIPTGP
                     TGHLAGTVLDARTEQPLEGVLVALIPARPTDFSLSRTPDSPIFRVTDENGQFAFRNLP
                     VGHYRVRATREDYLPAVQLAEVLEGEVTRLEFLLELQPLQLHGALAGVVTDAATSAPI
                     EGAWVRLAGDEHAWLSNTERLVTRTNAIGEYQFEHVPVGVYKVLVVKRGYEPGEGEAA
                     ILADQTTELNFALTPVEHAGAVEGVVTDAVTGAPIAQAIVFIPIVAEPLSANED"
BASE COUNT          365 a          965 c          719 g          334 t
ORIGIN      
        1 actccgcctg tctgccagga tctgggatag cgaggcgacg gcgcgcgccg aggtccaatc
       61 cggccagagc acccgcgtca acttcgcgct gcgtcccgat cccaatcccg acgccggcgt
      121 gattcagggg cgcgtgatcg accgcgaatc caatctgccc ctcgccggcg ccttcgtctt
      181 cttcgcgcgc ctgccgatca acaccgtcga accccccgcc gcgctcaacg agttcggcgc
      241 gatgatgtcg attgaacccc ccgccggcgg cgacgactac gtccgcacca acggccgcgg
      301 acgctaccag accccgcgcc tgcgccccgg cgtctatgtt gtcatcgcgt gggcgcccgg
      361 cttcgcctcg cagcatgtca tcgtgccgct gcccgtgggc ggcgccgccg aggccaattt
      421 cgccctccaa ccgctcgaac cgatgcaccc cggcgtgatt gaaggccgcg tcatcgaccg
      481 gctcaccggc gaccccgtgg ccggcgcctg ggtcttctat catccggcgg tcgacgacaa
      541 ggcgatcgcg ccccacgacg cccccgacgc cgcccatccc cgcgtccgca ccaacgcccg
      601 cggcgaattc tggatctctc cgctccgccc cggacgctac cacctggcgg tccgcgccga
      661 cggcttcgag ccggcgcaca agaacgtgaa cgtcgaggcg ggcgaggtca ccgcggtccg
      721 tttcctgctt gaaccgaagc ggccgccgca ccccggcgcg atcgtcggcc aggtcgtcga
      781 tggcgaaacg ggcgacccgg tccccggcgc gcgcgtcttc tttggccgtc cgctcgacga
      841 cgacgacacc gtcgaatggt ggcgcaagca cgacgcggcg accgaccacc tcaacttcgt
      901 cgaaaccgac catcagggct tctaccgcat caagccgctc cggcccggcg cctggcatct
      961 gctcgtgcgc gccgcgggct atcagccggc cgcccgcaag gtcgtcgtgc gcccgggcca
     1021 gacggtcgag gcgaatttca agctccgccc gaacgatccg cgtgatcccg gttcgatcgt
     1081 cggacgcgtg accgatggaa ccagccagac gccgctcgag ggcgcccgcg tgtactaccg
     1141 ccccatcccg cgcgacaccg tggaactgga cgagcgctcg cccgactcca ggttccaccc
     1201 ctcggtgatc gtcgacagcg agcggccccc cttcgtcgag accgatgcca acggccgctt
     1261 caagatcgag cacctgcggc ccggccgcta ccagttgctc gccgtcgccg cgggctacct
     1321 gccggcgagc gagatcgtcg ccgtcgagca gggccacacg accgaagcca gcttcgccct
     1381 ccgcccccgc cccgccggtc agggcgatct cgctggcatc gtcgtcgatg cccagacgca
     1441 tgacccgctg cgcggcgccc acgtctgggt cattccggcc ggcaacgtcg tcatcgccga
     1501 agcaatgatc cccgccgtca tcctcggcga ggcgatcacc gacaacaccg gccgctaccg
     1561 gatcgaggac ctgcccaccg gtccggtgat cgtcttcgcg gccagggaag gctaccgcac
     1621 cgcgcgcaaa cgcgtcgaaa tccccgccgg cggcgaggcg gtcgcccgct ttgccctcca
     1681 gccgatcccg accggaccga ccggccacct ggcggggacc gtcctcgacg cccgcaccga
     1741 acagccgctg gagggcgtcc tggtggccct gatccccgcc cggccgaccg acttctccct
     1801 ctcgcgcacg ccggactcac ccatcttccg cgtcaccgat gagaacggcc agttcgcctt
     1861 ccgcaacctc cccgtcggcc attaccgcgt gcgggcaacc agggaggatt acctccccgc
     1921 cgtccaactg gccgaggtgc tcgagggcga agtgacgcgc ctggaattcc tgctcgagct
     1981 ccaaccgctc cagctccacg gcgcactcgc cggcgtcgtc accgacgcgg ccacctccgc
     2041 gccgattgaa ggcgcctggg tccgcctggc cggcgacgag cacgcctggc tgagcaacac
     2101 cgagcgcctc gtgacccgca ccaacgccat cggcgaatac cagttcgagc acgtccctgt
     2161 cggcgtctac aaggtcctcg tcgtcaagcg cggctacgag ccgggcgagg gcgaagccgc
     2221 aatcctcgcc gaccagacca ccgagctcaa cttcgccctg accccggtcg aacacgccgg
     2281 cgccgtcgag ggcgtcgtga ccgacgccgt cacgggcgcg cccatcgctc aggccatcgt
     2341 cttcatcccg atcgtcgccg agcccctctc cgccaatgag gac
//
LOCUS       DAOSIF010000476         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38553_length_2380_cov_3.157849, whole genome shotgun sequence.
ACCESSION   DAOSIF010000476 DAOSIF010000000
VERSION     DAOSIF010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38553_length_2380_cov_3.157849"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLT35_12745"
     CDS             complement(<1..986)
                     /locus_tag="PLT35_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG30139.1"
                     /translation="MDRRDIRNFSIIAHIDHGKSTLADRFLELTGALQPREMTEAQVL
                     DSMDLERERGITIKAHPVRLGYEALDGRRYVLNLIDTPGHVDFSYEVTRSLAACEGAL
                     LLVDASQGVEAQTLANAYLAVEHNLEIIPVINKIDLPSAQPEEARRQLADIVGLDPSH
                     AISASAKAGTGVREILEAIVERLPPPAGDAAAPLKALIFDSWYDAYRGVVIVVRVIDG
                     VLRGRMKIRLMALGQEYDVEQVGVFTPKPVFVDELGAGEVGFFTATIKKVSDAKLGDT
                     VTDAARPARDPFPGFRELKPMVFAGLYPVENHQYPELRDALEGDRVLVRVLV"
     gene            complement(993..1409)
                     /locus_tag="PLT35_12750"
     CDS             complement(993..1409)
                     /locus_tag="PLT35_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30140.1"
                     /translation="MNAGRLAASAIVGWMAYLATSALVADVLLADLHARHAELFRPAG
                     EAHTVIGLGAALAGFFVFAYGYAKGYEGGPGALEGLRYGMVVGLLLVCFSVVWAWAAL
                     SLSGAIAAAWIVDTLVEMSLYGAIVGLVYAPGGSRK"
     gene            complement(1419..>2380)
                     /locus_tag="PLT35_12755"
     CDS             complement(1419..>2380)
                     /locus_tag="PLT35_12755"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG30141.1"
                     /translation="DYSTLTRVQAVLLDAHFVLEHGSRNRSGHFFNFNGTAGIWRRSV
                     IPAAGGWQHDTLTEDLDLSYRAQLLGWKFLFLPDLVAPAEVPVEMNAFKSQQHRWAKG
                     SIQVCLKLLPRILQSDQPFGVKAEAFLHLTANFNYLLMTALSVLMFPSMIIRYNMGWY
                     EMLLIDVPLFLAATASVVNFYLVSQRELYPDWKARLRYVPALMAVGIGLAVNNTRAVV
                     EALARHQSAFARTPKYGVERGSDQWVGKKYMQATAWQPLVETGLGLYFTATVLYALAN
                     GIYGSLPFLMLFQGGFLYMGLMSFIQQFGGAQAVLNAQVARGK"
BASE COUNT          394 a          822 c          819 g          345 t
ORIGIN      
        1 tcgacgagga cacgtacgag gacgcggtcg ccttcgaggg cgtcgcgcag ttccgggtac
       61 tggtggttct ccaccggata gaggcccgcg aacaccatgg gcttcagctc gcggaagccc
      121 gggaagggat cgcgcgccgg gcgcgcggcg tcggtcacgg tgtcgccgag cttggcgtcg
      181 ctgaccttct tgatggtggc ggtgaagaag ccgacctcgc cggcgcccag ttcgtccacg
      241 aacaccggct tcggcgtgaa cacgccgacc tgctcgacgt cgtactcctg gccgagcgcc
      301 atcaggcgga tcttcatgcg cccgcgcagg acgccgtcga tcacgcggac gacgatgacg
      361 acgccgcggt aggcgtcgta ccaggagtcg aagatgaggg ccttgagcgg cgccgccgcg
      421 tcgccggctg gaggaggcag ccgctcgaca atcgcctcga ggatctcccg gactccggtg
      481 cccgccttcg cgctggcgga aatggcgtgc gagggatcga ggccgacaat gtcggccagc
      541 tgccgccgcg cctcctcggg ctgcgcgctc ggcaggtcga tcttgttgat gaccgggatg
      601 atctccaggt tgtgctcgac ggccaggtac gcgttggcga gggtctgcgc ctcgacgccc
      661 tgcgacgcgt cgacgagcag cagggcgccc tcgcaggctg ccagcgaccg cgtgacctcg
      721 tacgagaagt ccacgtggcc gggggtgtcg atgaggttga gcacgtagcg ccggccgtcc
      781 agggcctcgt agccgaggcg caccggatgg gccttgatcg tgatcccccg ctcccgctcg
      841 aggtccatcg agtcgaggac ctgcgcctcg gtcatctccc gcggctgcag cgcgcccgtc
      901 agctcgagga acctgtccgc gagcgtggac ttgccgtgat cgatgtgggc gatgatcgag
      961 aagttgcgga tgtcgcggcg gtccatcgcg ggctacttgc ggctcccgcc gggcgcgtag
     1021 acgaggccga cgatcgcgcc gtagagggac atctccacga gcgtgtcgac gatccacgcg
     1081 gcggcgatcg cgccggacag cgacagcgcc gcccaggccc acaccaccga gaagcacacc
     1141 agaaggagcc ccacgaccat cccgtagcgc agcccctcca gcgcgcccgg accgccctcg
     1201 taccctttgg cgtagccgta cgcgaacacg aaaaagcccg caagcgccgc gcccagcccg
     1261 atgacggtgt gggcctcgcc agccggccgg aacagctccg cgtgccgggc gtgcagatcg
     1321 gccagcagca cgtcggcgac gagcgcgctc gtcgccaggt aggccatcca ccccacgatc
     1381 gccgatgcgg cgagccggcc ggcgttcacg gcgaggacct acttgccgcg ggccacctgg
     1441 gcgttcagca ccgcctgcgc gccgccgaac tgctggatga acgacatcag ccccatgtag
     1501 aggaacccgc cctggaagag catcaggaac ggcagcgacc cgtagatgcc gttcgccagc
     1561 gcgtagagga ccgtggcggt gaagtacagg cccagtcccg tctcgaccag cggctgccac
     1621 gccgtggcct gcatgtactt cttgcccacc cactgatcag agccgcgctc gaccccgtac
     1681 ttcggcgtcc gcgcgaaggc gctctggtgc ctggcgagtg cctcgacgac ggcccgcgta
     1741 ttgttcacgg ccaggccgat gcccaccgcc atcagcgccg gcacgtagcg cagccgggcc
     1801 ttccagtcgg ggtacagctc ccgctgcgac acgaggtaga agttgaccac cgacgccgtc
     1861 gccgcgagga acagcggcac gtcgatgagc agcatctcgt accagcccat gttgtagcgg
     1921 atgatcatgg acgggaacat gagcaccgac agcgccgtca tgagcaggta gttgaagttc
     1981 gccgtcaggt gcaggaacgc ctcggccttg acgccgaacg gctggtccga ctggaggatc
     2041 cggggcagca gcttgaggca gacctggatg gagcccttgg cccagcggtg ctgctgcgac
     2101 ttgaacgcgt tcatctcgac gggaacttcg gccggcgcca ccaggtccgg caggaagagg
     2161 aacttccagc ccaggagctg cgcccggtag ctcaggtcca ggtcctccgt gagcgtgtcg
     2221 tgctgccagc cccccgccgc cgggatcacc gaccggcgcc agatcccggc cgtgccgttg
     2281 aagttgaaga agtgccccga gcggttgcgg ctgccgtgct ccaggacgaa gtgcgcgtcc
     2341 agcaggacgg cctggacccg cgtcagcgtc gagtagtccg
//
LOCUS       DAOSIF010000477         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38661_length_2375_cov_3.646552, whole genome shotgun sequence.
ACCESSION   DAOSIF010000477 DAOSIF010000000
VERSION     DAOSIF010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38661_length_2375_cov_3.646552"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PLT35_12760"
     CDS             complement(<1..1139)
                     /locus_tag="PLT35_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG30142.1"
                     /translation="MTVLAALDSIRASAGPAATAGLPGDVVDRFASRDPRLGRAIDAA
                     RWRRTAMEASCGSLFRLSEPDLCSRLQERILNFYGAAGVNPYVPLAAEGPWIVTSHGA
                     VLHDSGGYGMLGLGHAPAAVLEAVAEPHVMANVMTPSFSQHRLTERLVREIGRSRGAC
                     PFDRFVFLNSGSEAVTFACRVSDIRARALTDPGGRRAGRTVKRLAVVDGFHGRTEGPA
                     RLSESCRAGYAKHLASFRGPDSLVFVPVNDIAALQQAFSDAEREGAFFEAMFVEPVMG
                     EGAPGLALTSEYYDAARSLTADSGSFLVVDSIQAALRAQGCLSIVDYPGFEASRPPDI
                     ETYSKALNAGQYPLSVVALTSEAAATYVTGLYGNTMTANPRAMEAA"
     gene            1457..1948
                     /locus_tag="PLT35_12765"
     CDS             1457..1948
                     /locus_tag="PLT35_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG30143.1"
                     /translation="MTAVLLAALLASCSSSTGSPASPSAAAAPAQALSPSTLEQQMHA
                     RVNAHRAAIGLPALSWHGGIADIARGHSANMAGGAVPFGHDGFAARVLAVALILRVAD
                     ASENLAMVSGYADPVAAVFQGWLDSPTHRPNVEGTFGQTGIGVAISPSGTVYFTQIFV
                     RTW"
     gene            complement(1967..>2375)
                     /locus_tag="PLT35_12770"
     CDS             complement(1967..>2375)
                     /locus_tag="PLT35_12770"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG30144.1"
                     /translation="FEGPDRAGVALRRRRVRTPQTAPEPREAERPKERPAPALVDTLI
                     YSSDDAGVVPPRLDRARLPAAPRVGTAQHDLPQVELVIAASGEVESVKLVTGPADVRA
                     AMMLSAVKNWQFRPATRDGVPVRYRMRLRLTNQ"
BASE COUNT          329 a          860 c          855 g          331 t
ORIGIN      
        1 gcggcttcca tggcgcgggg attggcggtc atggtgttcc cgtagagccc ggtgacgtag
       61 gtggcggccg cctccgacgt cagggccacg acggacagcg ggtactgccc ggcgttgagc
      121 gccttggagt aggtttcgat gtccggcggc cggctggcct cgaatcccgg gtaatccacg
      181 atgctgaggc agccctgggc gcgcaaggcg gcctggatgg agtccacgac gaggaacgac
      241 cccgagtcgg cggtcagcga gcgcgcggca tcgtaatact cggacgtcag tgcgagcccg
      301 ggcgccccct cccccatcac cggttcgacg aacatcgctt cgaagaacgc gccttcccgc
      361 tcggcgtccg agaaggcctg ctggagagcc gcgatgtcgt tcacgggcac gaacaccagg
      421 ctgtcggggc cgcggaacga cgccagatgc ttcgcgtagc cggcgcggca cgactcggat
      481 agccgcgcgg ggccttccgt gcggccgtgg aagccgtcga cgacggccag ccgcttgacc
      541 gtgcggccgg cccgccgccc gccgggatcg gtcagcgccc gggcgcggat gtccgagacg
      601 cggcacgcga aggtgacggc ctccgacccg ctgttcagga aaacgaacct gtcgaagggg
      661 cacgcgccac gactgcggcc gatctcgcgc acgagccgct cggtgaggcg gtgctggctg
      721 aacgaaggcg tcatcacgtt ggccatgaca tggggttcgg ccaccgcttc gagcacggcg
      781 gcaggcgcgt ggccgagccc gagcatgccg tagccgcccg agtcgtgcag caccgcgccg
      841 tgcgaggtga cgatccaggg cccttcggcg gccagcggca cgtacgggtt gacgcctgcc
      901 gcgccgtaga agttgaggat gcgctcctgc agccggctgc acaagtcggg ctcggagagc
      961 cggaacagcg agccgcagga ggcttccatg gccgtgcgcc gccaccgcgc cgcgtcgatg
     1021 gcgcgtccca ggcgcgggtc gcgcgaggcg aaacggtcga ccacgtcccc cggcagcccg
     1081 gcggttgcgg ccgggcccgc ggacgcgcgg atcgagtcaa gggcggcgag gacggtcatc
     1141 cgatgcatat cacctccggg ctcccgggcc atggccaacc cctgccgggg cggcgctcgt
     1201 cgtcacggcc ggatatgcac gagcacgcat aattattcaa tcatgccaca aaacgccggc
     1261 gcgcgcacga tccgccgccc ggcgacccgc ccgcccccgg ggtccgcccg tgaatggccc
     1321 cgcgccatac tggcgccctc cgccgcggcc gatacctccg taagggccgc cggcccgcac
     1381 ggggcccggc cgacttcccc ttccgggggc actgcccatg cgccgcgttc caccctcgcc
     1441 gtcgaccgcg cccgtcgtga cggccgtcct cctcgcggcg ctgctcgcgt cctgctcgtc
     1501 gtccaccggg tctccagctt ccccgtcggc cgcggccgct cctgcccagg cgctttcgcc
     1561 ctcgaccctt gaacagcaga tgcacgccag ggtcaacgcc catcgcgcgg cgatcggcct
     1621 gccggccctc tcctggcacg gcgggatcgc ggacatcgcc cgaggccaca gtgcgaacat
     1681 ggccggcggc gccgtgccgt tcggccacga cggcttcgcg gcgcgggtct tggctgttgc
     1741 gctgatcctc cgcgttgcgg acgcctccga gaatctcgcg atggtgtcgg gctacgcgga
     1801 tccggtcgcc gctgtgttcc aggggtggct ggacagtccg acccacaggc ccaacgtcga
     1861 agggaccttc ggtcagaccg gcatcggcgt ggcgatctcc ccctccggga cggtctactt
     1921 cacgcagatc ttcgtccgca catggtgaag agcggggcca ggctggctac tggttggtca
     1981 gccgcaggcg catcctgtag cggacgggaa cgccgtcgcg ggtggccggg cggaactgcc
     2041 agttcttgac ggcactcagc atcatcgctg cgcggacgtc ggcgggcccg gtgacgagct
     2101 tgaccgattc cacctcgccg gaggcggcaa tgacgagttc cacctgaggc aggtcgtgct
     2161 gcgcggtgcc gacgcgaggg gccgcgggca ggcgcgcgcg gtccagccgc ggcggcacca
     2221 cgccggcgtc gtcgctggag tagatcaggg tgtcgacaag cgccggcgcc ggccgttcct
     2281 tgggacgctc cgcctcgcgg ggctcagggg ccgtctgggg agtccgcacc cgccgccggc
     2341 gcagcgccac gccggcgcgg tcggggcctt cgaag
//
LOCUS       DAOSIF010000478         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38691_length_2374_cov_3.492022, whole genome shotgun sequence.
ACCESSION   DAOSIF010000478 DAOSIF010000000
VERSION     DAOSIF010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38691_length_2374_cov_3.492022"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..675)
                     /locus_tag="PLT35_12775"
     CDS             complement(127..675)
                     /locus_tag="PLT35_12775"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG30145.1"
                     /translation="MLIALVVVIVLVVAIAGMYNALVRLRVLVDNAWADIDVQLKRRH
                     DLIPNLVETVKGYASHEKSTFEEVVAARAKAMSAQTPEARGQAELGLSAALGRLFALA
                     EAYPQLRAVESFTQLQGTLAQIEDAVQSARRYYNAVVRDFNTKTAQVPWNILASTFGF
                     ASREFFQLTDENERAVPSVTFS"
     gene            882..2051
                     /locus_tag="PLT35_12780"
     CDS             882..2051
                     /locus_tag="PLT35_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG30146.1"
                     /translation="MSGLRTRFGRPLRLGMIGGGPDSWIGRVHQGGATLDGWWNVVAG
                     VWSGDPERSRLAGPSLGVDASRSYGDVSEMLARERERPDGIEAVAIMTPNDTHYPYAA
                     AALDAGLDVLCDKPVTHNLAQARDLAAKVRRSGRLFAITHGYSAYPMIRYASRLVREG
                     ALGALRLVQVEYIQAGLAARLEDAPPSNRLRWLLDPRRSGLAQVMSAIGCHAQHLACF
                     VTGYAVARVSAEAAPTVPGRRLIDHVSALMEFDGGCYGTFTAFQAAAGSENDIRLRVY
                     GERGLIDWNHRECSYLRVAMQGEAARVVARGDADLPPEILALGRAPRGHPEGLLQAFA
                     NLYGEVAQERMAIALGDARPAFPYPRIEDGAHSMAFIEACLASKAAGTWVDVERP"
     gene            2151..>2374
                     /locus_tag="PLT35_12785"
     CDS             2151..>2374
                     /locus_tag="PLT35_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30147.1"
                     /translation="MATCDRREFLSCVAAGVAASAAPRTAAPGRARSAVCSLSPPAFA
                     PLPLGSIRPSGWLLRQLRIQADGLSGHLDQ"
BASE COUNT          345 a          830 c          841 g          358 t
ORIGIN      
        1 ccgacttcga tggcggcggc gaactcctga atcacgagcg tccgctgcgc ggcggcaggc
       61 gaagcgagcg tccagaggca cgccgcggca atcgcgagcc tcgagcccgt catgatggcc
      121 tccgacctag ctgaaggtga ccgaaggcac cgcccgctcg ttctcgtccg tcagctggaa
      181 gaactcgcgg gaggcgaacc cgaacgtcga cgccaggatg ttccacggca cttgggcggt
      241 cttcgtattg aaatcgcgca cgacggcgtt gtagtaccgg cgcgcggact gcacggcgtc
      301 ctcgatctgc gcgagcgtgc cctggagctg ggtgaagctc tcgacggcgc gcagctgcgg
      361 gtaggcctcc gccagggcga acagtctgcc cagtgcggcg ctcagcccga gctcggcctg
      421 gccgcgcgcc tcgggggtct gcgccgacat cgccttcgcc cgcgccgcga cgacctcctc
      481 gaacgtgctc ttctcgtgcg acgcgtaccc cttcacggtc tcgacgaggt tcggaatgag
      541 atcgtgccgc cgcttgagtt gcacgtcgat gtcggcccac gcgttgtcca ccagcaccct
      601 cagacggacc agggcgttgt acatcccggc aatcgcgacg acgaggacga tgacgacgac
      661 gagagcgatc agcatgtgga tgctcccggc ggctgcgccg gtgccgcctc atgaacccgt
      721 ggtcgccgcc gctgccgcgg gcgcagcggc aagcggtatt cacgtaacag aatacacccg
      781 cgggcgggtt gccgcgcgga acgagggagc ggcgcgcgcc gggtcgggta caatgcgcct
      841 gcgcggcgga gaggcgcgcg gcgcgggcgg aggcgcagag catgagcgga ctcagaacgc
      901 ggttcgggcg gcccctgcgg ctcggcatga tcgggggcgg ccccgactcc tggatcggcc
      961 gagtgcacca gggcggcgcc actctggacg gatggtggaa cgtggtggcg ggcgtatggt
     1021 cgggcgatcc cgagcggtcg cgcctcgcgg gcccctcgct cggcgtggac gcctcccgct
     1081 cgtacggcga tgtgtccgag atgctggccc gggaacgcga gcggcccgac ggcatcgagg
     1141 ccgtcgcgat catgacgccg aacgacacgc actatccata cgccgccgcg gccctcgacg
     1201 ccggcctcga cgtgctgtgc gacaagcccg tgacgcacaa cctcgcccag gcccgcgatc
     1261 tggcggcgaa ggtgcgcagg tcggggcggc tgttcgccat cacgcacggc tattcggcct
     1321 atccgatgat ccgctacgcc tcgcgcctag tgcgcgaggg cgcgctgggc gcactgcgcc
     1381 tcgtgcaggt ggagtacatc caggccgggc tcgccgcgcg cctcgaggac gcgccgccgt
     1441 ccaaccggct gcgatggctc ctcgatcccc ggcggagcgg actggcgcag gtcatgagcg
     1501 ccatcggctg ccacgcccag catctggcct gcttcgtgac cgggtatgcc gtcgcccggg
     1561 tctcggccga agccgcgccg acggtccccg gccgtcggct catcgaccac gtatcggccc
     1621 tcatggagtt cgacggtggc tgctacggca cgttcaccgc gttccaggcg gcggcaggga
     1681 gcgagaacga catccgcctt cgcgtctacg gcgagcgggg cttgatcgac tggaaccacc
     1741 gggagtgcag ctacctgcgc gtggcgatgc agggcgaggc cgcgcgggtc gtcgcccgcg
     1801 gcgatgccga cctcccgccc gagattctcg ctctgggccg ggcgccgcgc ggccacccag
     1861 agggcctgct ccaggcattc gccaatctgt acggcgaggt ggcgcaggag cgcatggcaa
     1921 ttgcccttgg cgacgcgagg cccgcgtttc cctacccgcg catcgaggac ggagcccact
     1981 cgatggcctt catcgaggcg tgcctcgcgt cgaaggctgc tggcacctgg gtcgacgtcg
     2041 agcgtccgtg aacgcgaaag gggtcgggac gattttcgca accgacaatt ccgtcataca
     2101 gaattgtcgg ttgcgaaaat cgtcccgacc cctttgtatg gagggacacg atggcaacgt
     2161 gtgaccggcg cgagttcctg tcgtgtgtcg cggccggagt agccgcgtcg gctgccccca
     2221 gaaccgccgc gcccggacgc gcgcgctcgg ccgtgtgctc gctgtcaccg ccggcgttcg
     2281 cgccgctgcc gctcgggtca atccgccctt cgggctggct cctgcgccag ctgcgcatcc
     2341 aggccgacgg gctctcgggc catctggacc agtt
//
LOCUS       DAOSIF010000479         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38843_length_2368_cov_3.293558, whole genome shotgun sequence.
ACCESSION   DAOSIF010000479 DAOSIF010000000
VERSION     DAOSIF010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38843_length_2368_cov_3.293558"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2332)
                     /locus_tag="PLT35_12790"
     CDS             complement(<1..2332)
                     /locus_tag="PLT35_12790"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30148.1"
                     /translation="MHAAVLQALEFTRIVDVLRGCAATPLGAERLANLKPATDPRKVA
                     QQLAATSEGVRFNELVGGLALSAPADLAAILGNLAVEGRPLEALRLLALADYLDSVDQ
                     ARGAIRRTEHPFPVLKALAEGVASFRSEIADTRHKIEPSGEVADGASPDLKAIRERLR
                     RQRTRLRGTLESFLRNRDTAKYLQDQVVTDRNGRFVLVVRAEHRTAIPGIVHGSSSSG
                     ASLYLEPLSTVEINNDIVALEQQEAEEVRRVLLGLTDQYRRRALDLQRTIESATDLDV
                     IQAKARLSAIVGGAEPALSTDGAFELLAARHPLLIPAVADRLAARPSAAGEGGAGSDA
                     APPAAPRVEGPVPVDIKLVPGTSVLVVTGPNTGGKTVALKTAGLLALMAQAGLHVPAH
                     TGSRVPVFRSVFADIGDEQSIAANLSTFSWHVANMVQTDRSLALPALVLLDEPGAGTD
                     PIEGGALGLAVVEHFRARGAHVVATTHHEPLKSYASTTPGVVCAAFGFDPESFAPTYR
                     LQYGTPGRSLALEIAGRLGLNPSILESARSNVSAREAQLAEHLAKIDADIRALDHERR
                     LVARERESMAEADMRLKAREQAVRDREERVRQRADEEIATRVRAAREDIDQVVEGLRR
                     EIDRLTAEAGRRALHGRPLSTGEAGTARSQARAALDQVEARLGDGREAAPPPAQPQGP
                     AARVGDRVALRGLGVEGRVLSIQAGDAEIDVRGKRLRARLAELRVIGGPAPSEPPHVR
                     VNVQVAPRDAPQTEINVIGCSVDEAIGRIERFLDDLL"
BASE COUNT          301 a          878 c          867 g          322 t
ORIGIN      
        1 ggagcaggtc gtcgaggaac cgctcgatcc ggccgatggc ctcgtccacc gagcagccaa
       61 tgacgttgat ctccgtctgg ggcgcgtccc gcggcgcgac ctggacgttg acgcgcacgt
      121 gcggcggctc ggacggggcg ggccctccga tgacgcgcag ctcggcgagc cgggcgcgga
      181 ggcgcttgcc ccgcacgtcg atctccgcgt ctcccgcctg gatcgacagc acgcggccct
      241 cgacgccgag cccccgcagc gccacccgat cgccgacccg cgccgccggg ccctgcggct
      301 gggcgggcgg cggcgcggcc tcgcggccgt cgccgagccg cgcctccacc tggtccagcg
      361 cggcccgcgc ctggctgcgc gccgtcccgg cctcgcccgt ggacaacggg cggccgtgca
      421 gggcgcggcg gcccgcctcg gccgtcagcc gatcgatctc gcggcggagc ccctccacga
      481 cttggtcgat gtcctcgcgg gccgcgcgaa cgcgcgtcgc gatctcctcg tccgcacgct
      541 ggcgcacccg ctcctcgcga tccctgaccg cctgctcccg cgccttcagg cgcatgtccg
      601 cctcggccat cgactcgcgt tcgcgggcga ccaggcgccg ctcgtgatcg agggcgcgga
      661 tgtcggcgtc gatcttcgcg agatgctcgg ccagctgggc ctcgcgcgcc gacacgttgc
      721 tgcgggcgct ctccagaatc gacggattca agccgagccg cccggcgatc tcgagggcga
      781 ggctgcggcc gggcgtgccg tactgcagcc ggtaggtcgg cgcgaacgac tcgggatcga
      841 agccgaacgc ggcgcacacg acgcccggcg tcgtcgaggc gtacgacttc agcggctcgt
      901 ggtgcgtcgt cgccacgacg tgcgcgccac gggccctgaa gtgctccacc accgccagac
      961 cgagcgcgcc gccctcgatc gggtcggtgc ccgcgccggg ctcgtcaagc agcaccaggg
     1021 cgggcagcgc cagcgaccgg tccgtctgga ccatgttggc gacgtgccac gagaacgtgc
     1081 tgaggttcgc ggcaatcgac tgctcgtcgc cgatgtcggc gaacaccgac cggaacacgg
     1141 gcactctgga ccccgtgtgg gccggcacgt gcagaccggc ctgcgccatc agcgccagga
     1201 gcccggccgt cttgagcgcg acggtcttgc cgccggtgtt cgggcccgtg acgaccagga
     1261 cgctcgtccc cgggacgagc ttgatgtcca ccggcaccgg gccctccaca cggggggcgg
     1321 ccggaggggc cgcgtcggag cccgcgccgc cctcccccgc ggcggagggc cgcgcggcga
     1381 gccggtcggc gaccgccggg atgagcagcg ggtgccgggc ggccagcagc tcgaaggcgc
     1441 cgtccgtcga cagggccggc tcggcgccgc cgacgatcgc cgacagccgc gccttggcct
     1501 ggatgacgtc caggtccgtc gccgactcga tcgtccgctg caagtcgagc gcgcgccgcc
     1561 ggtactggtc cgtcaggccc agcaggacgc gccggacttc ctccgcctcc tgctgttcga
     1621 gggcgacgat gtcgttgttg atctcgaccg tgctcagcgg ctccaggtac aggctcgcgc
     1681 cgctcgacga gctgccgtgc acgatcccgg gaatggccgt gcgatgctcg gcgcgaacca
     1741 ccagtacgaa tcggccgttg cggtcggtga cgacctggtc ctgcaggtac ttggcggtgt
     1801 cgcggttccg caggaaggac tcgagcgtgc ctcgcagccg cgtgcgctgg cgccgcagcc
     1861 gttcgcggat ggccttgagg tcggggctcg cgccgtccgc gacctcgccg gagggctcga
     1921 tcttgtggcg cgtgtcggca atttcgctcc tgaaggaggc aacgccctcg gccagcgcct
     1981 tcagcacggg aaacgggtgc tcggtccgcc ggatggcgcc ccgggcctgg tcgaccgagt
     2041 ccaggtagtc cgccagggcc agcagccgaa gggcctcgag cgggcggccc tcgacagcca
     2101 ggttgccgag gatggccgca aggtccgcag gcgccgacag ggcgagcccg cccaccaatt
     2161 cgttgaaccg gacgccctcg gacgtggcgg ccagctgctg ggcgaccttt ctcgggtcgg
     2221 tggccggctt gagattcgcc agccgctcgg caccgagcgg cgttgcggcg caccctcgca
     2281 ggacgtcaac gatgcgcgtg aattcgaggg cctgcaggac ggctgcgtgc atagaaatcg
     2341 cgaactgcgg tggagaggtc gcgggccc
//
LOCUS       DAOSIF010000480         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38937_length_2364_cov_3.847553, whole genome shotgun sequence.
ACCESSION   DAOSIF010000480 DAOSIF010000000
VERSION     DAOSIF010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38937_length_2364_cov_3.847553"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLT35_12795"
     CDS             complement(<1..1285)
                     /locus_tag="PLT35_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG30149.1"
                     /translation="MAARPGSAPVPRLSLHRLGVVCGLAAGAGLGAAEAPTKLVTTGL
                     SPFLISLGMVAGVFVARWTVPMVLKGGDYVWADLREKPHLVVWAILAGMIWAVANTLT
                     VFAIRDVGLSIAFPLWNTNSLVGLFWGWLLFRELRDAGAGQWGKVLGGATAIVVGACL
                     TAYATAHQAPGSRESAAMGIAAALMAGLLWGTMYIPYRKAYISGMNPLSFVTVFTFAE
                     LGTVLVLAAVFEGGLRPLAAALAQARPALFWLFLGGFCWVLGDLYQQYAAKYVGIGRG
                     IPLSNTNQLWGLAWGALVFGEFAGLGAAGRALIVSGSLIMIAGAIAISLAEAPTSERA
                     EWRAAMRRECDRYGLDPAALESALHGEDRLAAGVSRRRTWEYVVAALAVALFVWLASF
                     AERPQIPIHGAWTLLLGAITALVIVLIFGAVVIAWL"
     gene            complement(1624..2079)
                     /locus_tag="PLT35_12800"
     CDS             complement(1624..2079)
                     /locus_tag="PLT35_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30150.1"
                     /translation="MSGSRRRLLAAGLAALALAALAGGACSPSVPAPQAVRLGHATCA
                     KCRHVVSSLDAAAQAVFTDGSVRVYDDLGCMATDPEALRGRAQLYVQLAGGRGWVRVE
                     DICFASPPGTRTPRGYNYLAFPEEEARQLAPDRWARGWHDLVAELARAR"
     gene            complement(2076..>2364)
                     /locus_tag="PLT35_12805"
     CDS             complement(2076..>2364)
                     /locus_tag="PLT35_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30151.1"
                     /translation="ERSLCGAVEESPRGVPEWHWPPYWPGGTVQAKVMDSAMARRLEL
                     WAAAGRPCAGDFLAEPFLERRPQFAWMRGLLRDMTSGPWTRFAAGMSEAAR"
BASE COUNT          367 a          925 c          809 g          263 t
ORIGIN      
        1 gcagccacgc gatcacgacc gcgccgaaga tgagcacgat caccagcgcc gtgatcgcgc
       61 cgaggagcag cgtccaggct ccgtggatcg ggatctgcgg gcgctcggcg aacgacgcca
      121 gccagacgaa cagcgcgacg gctagggccg cgacaacgta ctcccacgtg cgccggcggg
      181 acacgcccgc cgccaggcga tcctctccgt gcagcgccga ctcgagcgcc gccggatcga
      241 ggccgtaccg gtcgcattcg cgcctcatcg ccgccctcca ttcggcgcgc tccgacgtcg
      301 gcgcctcggc caggctgatt gcgatcgcgc cggcgatcat gatgagcgag ccgctcacga
      361 taagggctcg cccggcggcg ccgagccccg cgaactcgcc gaagacgagc gcgccccagg
      421 ccagtcccca gagctggttg gtgttcgaga gcgggatgcc ccggccgata ccgacgtact
      481 tcgccgcata ctgctgatac aggtcaccga gcacccagca gaagccgccg agaaacagcc
      541 agaagagcgc cggcctggcc tgcgccagcg ccgccgcgag gggccgcagg ccgccctcga
      601 acaccgctgc cagcaccagc acggtgccga gctcggcgaa agtgaacacc gtcacgaacg
      661 acagcgggtt catgccgctg atgtacgcct tgcgatacgg gatgtacatc gtgccccaga
      721 gcaggccggc catgagcgcg gccgcaatgc ccatggccgc gctctcgcgg ctgcccgggg
      781 cctggtgcgc tgtcgcgtag gccgtcaggc aggccccgac cacgatggcg gtcgcgcctc
      841 ccagcacctt gccccactgc cccgcccccg cgtcccgcag ctcccggaac aggagccagc
      901 cccagaacaa gccgacgagg ctgttcgtgt tccagagcgg gaacgcgatc gacagcccga
      961 cgtcccgaat cgcgaagacc gtcagcgtgt tggcgaccgc ccagatcatg ccggcgagga
     1021 tcgcccagac gacgaggtgc ggcttctccc gcaggtcggc ccagacgtag tcaccgccct
     1081 tcagcaccat cgggaccgtc cagcgcgcga cgaagacgcc ggcgaccatg cccagcgaga
     1141 tcaggaacgg cgacagcccg gtggtcacca gtttcgtcgg ggcctcggcg gcgccgaggc
     1201 cggcgccggc ggccaggccg cagaccacgc caaggcggtg gagagagagg cggggcaccg
     1261 gcgcggagcc gggacgggcg gccacgagtc acctcaacga aacgagcagg atgacgccga
     1321 cgagcagcac ggcggcggga atccagaact gcacgtcggt gacaatcgca cggccgagcg
     1381 agcgtttcct cacgggcctt cctccccgcc caggacgggc gctctgcact gtatcaggag
     1441 atcgccggtc cccgcacgcg ccgatgcgca cgcgccccgc gccgcgtctg catcgcgccg
     1501 gcgctccgcc ggcgccggac ttccgcctcc ggcgtctccc gggccgtccc gggcctccgc
     1561 cgacgccgcg gggcagcgga ctgtcgtccg gccccagtgg gcgtttcgcc tcgggcgagc
     1621 ggctcacctc gcgcgcgcca gctccgccac caggtcgtgc cagcccctgg cccaccggtc
     1681 gggcgcgagc tgtcgcgcct cctcttcggg gaacgcgagg tagttgtacc ccctcggcgt
     1741 ccgggtgccg gggggcgagg cgaagcagat gtcctccacg cgaacccagc ccctgccgcc
     1801 ggccaactgc acgtacagct gcgcgcggcc gcggagcgcc tccgggtcgg tcgccatgca
     1861 tccgaggtcg tcgtacacgc gcacggagcc gtcggtgaag acggcctgtg cggccgcgtc
     1921 gaggctcgac acgacgtgcc ggcacttcgc gcaggtcgcg tgtcccaacc ggacggcctg
     1981 cggagcgggg acgctcggcg agcacgcccc gcccgcgagc gccgcaagcg ccagcgccgc
     2041 gaggcccgct gccagaagcc ggcgccgcga gccgctcacc gggccgcctc gctcatgccg
     2101 gccgcgaacc gcgtccacgg cccgctcgtc atgtcgcgga ggagcccgcg catccaggcg
     2161 aactggggcc gccgctccag gaacggctcg gccaggaagt cccccgcgca cggccgtcct
     2221 gccgccgccc acagctcgag gcgccgcgcc atggccgagt ccatcacctt cgcctgcacc
     2281 gtgccgccgg gccagtacgg cggccagtgc cattcgggca ccccgcgcgg cgactcctcg
     2341 accgcgccgc acagggaccg ctcg
//
LOCUS       DAOSIF010000481         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_38940_length_2364_cov_2.982243, whole genome shotgun sequence.
ACCESSION   DAOSIF010000481 DAOSIF010000000
VERSION     DAOSIF010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38940_length_2364_cov_2.982243"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLT35_12810"
     CDS             complement(<1..468)
                     /locus_tag="PLT35_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30152.1"
                     /translation="MVSLVLLVGLLLDPLAPPPPVQAPTPQASPSQAPPNQAAPGGIV
                     DGKGGPSFVVPRIDEVVSIDGRLDEPAWSRAVRLGGFSEYQPVDSRPATERTEVLVWY
                     SPKALHIGIVAFDSEPGSVRANVSDRDNIANDDWVRIFLDTFNDRRRAFLFGVN"
     gene            620..1003
                     /locus_tag="PLT35_12815"
     CDS             620..1003
                     /locus_tag="PLT35_12815"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOG30153.1"
                     /translation="MPYRVLAAAVVLLHLAFVVFVVAGGLLVLRRRWAAAVHLPAAAW
                     GVFVEFSGRICPLTPLENRLRALGGGAAYSGDFVDQYLMPVLYPPGLRRGVQVALGLF
                     ALAVNAAVYAYACRSARRRRMGGRR"
     gene            complement(1035..2240)
                     /locus_tag="PLT35_12820"
     CDS             complement(1035..2240)
                     /locus_tag="PLT35_12820"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOG30154.1"
                     /translation="MMGPFVPDPISNELNLVIAFLLGTGFGVTLEQAGFSSSRKLAGV
                     FYGYDFTVLRVFFTAAVTAMSGILLFGYLGLLDLDVIFINPTWLAPAIVGGAIMGAGF
                     ILGGYCPGTSLCAAVIGKVDAMFFVLGGAIGVFGFGELYPLYKGFYESTALGELRVFD
                     SIGVSQGLFAFLLIAAAVAAFAVTTIIEKRVAGAAAPSSEFKASRHAAAGAALLAVGL
                     VLLVLPDRKTSMIARASDPAILAAHPAATMEIDELAYRLIDRDPRVQVVDIRPPEAFA
                     RQSLPGAKNLPIRQFFGQDAMAVLSPRHVKKVIVGETEAEEHVASRLMIDLGYENAVV
                     LKGGFAAFQAAYLGPSPPAPVEGLSRWAADLTRFRTQARVDLLQLIASARAEAPKAPK
                     PQKKIAGGC"
BASE COUNT          351 a          844 c          842 g          327 t
ORIGIN      
        1 gttgacgccg aagaggaacg cccggcggcg gtcgttgaag gtgtcgagga agatccgcac
       61 ccagtcgtcg ttggcgatgt tgtcgcggtc cgagacgttg gcgcggacgg acccgggctc
      121 cgagtcgaag gcgacaatgc cgatgtggag cgccttcggc gagtaccaga cgaggacctc
      181 cgtccgctcc gtcgccggcc ggctgtccac cggctggtac tcggagaagc cccccagccg
      241 caccgcccgc gaccacgccg gctcgtcgag gcggccgtcg atcgacacga cctcgtcgat
      301 gcgcggcacg acgaacgacg gcccgccctt gccgtccacg atgccgcccg gggccgcctg
      361 gttcggcggc gcctgcgatg gcgaagcctg cggcgtgggc gcctggaccg gaggcggagg
      421 cgccagggga tcgaggagga ggccgacgag caggaccaga gacaccatcg gtttggaatc
      481 cggggaatgc cgatcgcgcc caggccgagc ggagcgggcc tcccagtcta cgcgattccc
      541 aggcccggtg tcagccgggc cgcggcgccg gtgcgcacct cgcaggcctg tgcgctagga
      601 tgaggcggag ttggcaacga tgccctaccg ggtcctggct gcggccgtcg tcctgctgca
      661 cctcgcgttc gtcgtgttcg tggtcgccgg cggtctgctc gtccttcggc ggcggtgggc
      721 ggcggccgtg caccttcccg ccgccgcgtg gggcgttttc gtcgagttca gcggccgcat
      781 ctgcccgctg acgcccctcg agaaccgcct gcgcgcgctc ggcggcggcg cggcctattc
      841 gggcgacttc gtcgaccagt acctgatgcc ggtcctctac ccgcccggcc tccgccgcgg
      901 cgtccaggtc gccctcggcc tcttcgccct ggccgtcaac gccgccgtgt acgcctacgc
      961 ctgtcggagc gcccgccggc gcaggatggg cgggcgccgg tgactgcggg aggacgcggg
     1021 cccgccgcgc cgcgctagca gcccccggcg atcttcttct gcggcttcgg cgccttcggc
     1081 gcctccgccc tggcgctcgc gatgagctgc agcaggtcga cgcgcgcctg cgtgcggaac
     1141 cgcgtgagat cggcggccca gcggctgagc ccttccaccg gagcgggcgg cgagggcccc
     1201 aggtacgccg cctggaaggc cgcgaagccg cctttcagca cgaccgcgtt ctcgtagccc
     1261 aggtcgatca tcagccggct tgcgacgtgc tcttcggcct ctgtctcccc cacgatgacc
     1321 ttcttgacgt gcctcggcga cagcaccgcc atcgcgtcct ggccgaagaa ctgccggatg
     1381 ggcaggttct tcgcgcccgg cagcgactgc cgcgcgaagg cctcgggcgg ccggatgtcc
     1441 accacctgca cgcgcggatc gcgatcgatc agccggtagg ccagctcgtc gatctccatc
     1501 gtcgcggccg ggtgggccgc caggatcgcc gggtccgacg cgcgcgcgat catgctcgtc
     1561 ttgcggtcgg gcaggaccag gagcacgagg ccgaccgcca gcagcgccgc gccggccgcc
     1621 gcgtgccggc tcgccttgaa ctcgctcgag ggcgccgccg cgcccgccac gcgcttctcg
     1681 atgatcgtgg tcacggcgaa cgcggccacc gccgcggcga tcagcaggaa ggcgaagagg
     1741 ccctgcgaga cgccgatcga gtcgaacacc ctgagttcgc cgagggcggt ggactcgtag
     1801 aaccccttgt agagcgggta cagctcgccg aagccgaaca cgccaatcgc gccgcctagc
     1861 acgaagaaca tcgcgtcgac cttgccgatg acggcggcgc acaggctcgt ccccgggcag
     1921 tagccgccga ggatgaagcc cgcgcccatg atggcgccgc cgacgatggc cggcgccagc
     1981 caggtgggat tgatgaagat gacgtcgagg tcgagcaggc cgaggtagcc gaagagcagg
     2041 atgccgctca tggccgtcac cgccgccgtg aagaacacgc gcagcacggt gaagtcgtag
     2101 ccgtagaaca ccccggcgag cttgcgcgac gaggagaagc ccgcctgctc gagggtgacg
     2161 ccgaagccgg tgccgagcag gaacgcgatg acgaggttca gctcgttcga gatcgggtcg
     2221 ggcacgaacg gtcccatcat gtgagcgccc tcccctgctc agatccagag cctgcgggcg
     2281 aagaacgcca gcaggtagcc ggtcccgaag atggccatca tcgtgacgaa cccggcagtg
     2341 gacaggacgg ccatgccgct caag
//
LOCUS       DAOSIF010000482         2360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_39019_length_2360_cov_3.247722, whole genome shotgun sequence.
ACCESSION   DAOSIF010000482 DAOSIF010000000
VERSION     DAOSIF010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39019_length_2360_cov_3.247722"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="PLT35_12825"
     CDS             <1..1599
                     /locus_tag="PLT35_12825"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOG30155.1"
                     /translation="QDDQPGRYHTIGDPTEGALVVAAAKFRLRKPDLDLRFPRVAEAP
                     FDSDRKRMTTVHEMPAAPPPAARSLDWLWGQEPPPAPSRYVAVTKGAVEPLVQISTRV
                     WADGRPQPMDDEWRARIRTAHDRLADDGARVLGLAFRLLGDLPAGGAPALERDLVFVG
                     LIGMSDPPRPEVKNAVATCRQAGIRPVMITGDHPLTAKYVARQLGIGDEDARVVLGQD
                     LQRLSDDELRRMVDGAAVYARVSPEHKLRVVQALQANGGVVAMTGDGVNDAPALRRAD
                     IGVAMGITGTEVSKEAADMVLTDDNFATIVAAVEEGRVIYDNIRKFIRYLLSSNSGEL
                     WVMLIAPFVGLPLPLLPLQILWINLVTDGLPALALSVEPPERHAMQRPPIPPTESILG
                     RGLLWQVIWGGLVLGLTPLLMGYYYHGIGHEGWRTMVFTTLTLSQLVLVLSLRSERDS
                     FFTIGAFSNAPLLAIVGLTFALQMMVTYAPFWQRVLKTHALTAEELGVSLAMATVPFA
                     ADEVRKWVRRARRASKGGGADASP"
     gene            1613..2341
                     /locus_tag="PLT35_12830"
     CDS             1613..2341
                     /locus_tag="PLT35_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOG30156.1"
                     /translation="MRTEYHRWYSGRVGREMGLVVYGHWGPPMLVFPTSGGDEWEMER
                     QGMVAALADFIDPGRVKVFTIDANSRDSFYNRGAHPFHRSYMQAQYDAYVREEVVPFI
                     RRSCQSDDIAITTAGASLGAYHAANTLFKHPDAVRRCYALSGVYDLKRFMDGMYDDNF
                     YFNNPVDYMSNLDDPRLREWLGGCDIHLATGHGPWEDKGPTYRMAEVLRTKGIPHSLD
                     DWGGMGGHDWPFWKHQMRTYLSAW"
BASE COUNT          356 a          818 c          860 g          326 t
ORIGIN      
        1 caggacgacc agccggggcg ctaccacacg atcggcgacc cgacggaagg cgcgctggtg
       61 gtggcggccg cgaagttccg cctgcgcaag cccgacctcg acttgcgctt cccgcgcgtg
      121 gcggaggcgc cgttcgactc ggaccgcaag cggatgacga cggtgcacga gatgccggcg
      181 gcgccgccgc ccgccgcccg cagcctcgac tggctgtggg gccaggagcc gccgcccgcg
      241 ccgagccgct acgtcgcggt gaccaagggc gccgtcgagc cgctggtgca gatctccacg
      301 cgggtctggg ccgacggccg gccccagccg atggacgacg agtggcgcgc gcgcatccgg
      361 accgcgcacg atcgcctcgc cgacgacggc gcgcgcgtgc tcgggctggc cttccgcctc
      421 ctcggcgacc tgccggcagg cggcgcgccc gccctcgagc gggacctcgt cttcgtcggc
      481 ctgatcggca tgagcgatcc gccgcgcccc gaggtgaaga acgcggtggc aacgtgccgg
      541 caggcgggaa tccggccggt gatgatcacc ggggaccacc cgctgacggc gaagtacgtg
      601 gcccggcagc tcggcatcgg cgacgaggac gcgcgggtcg tgctcggcca ggacctgcag
      661 cgcctctccg acgacgagct gcggcgcatg gtggacggcg ccgccgtcta cgcgcgcgtg
      721 tcgcccgagc acaagctgcg cgtcgtccag gcgctgcagg ccaacggcgg cgtcgtcgcg
      781 atgacgggcg acggcgtgaa cgacgcgccg gccctgcggc gcgccgacat cggcgtggcg
      841 atgggcatca cggggacgga ggtgtcgaag gaggcggccg acatggtcct caccgacgac
      901 aacttcgcca ccatcgtcgc ggcggtggag gaaggccggg tcatctacga caacatccgc
      961 aagttcatcc gctacctgct gagcagcaac tcgggcgagc tgtgggtgat gctgatcgcg
     1021 ccgttcgtcg gcctgccgct ccccctgctg ccgctgcaga tcctgtggat caacctcgtg
     1081 accgacggcc tgcccgcgct ggcgctgagc gtcgagccgc ccgagcgcca cgcgatgcag
     1141 cgcccgccga tcccgccgac ggagagcatc ctcggccggg gcctgctgtg gcaggtgatc
     1201 tggggcgggc tggtgctggg cctcacgccg ctcctcatgg gctactacta ccacggcatc
     1261 ggccacgagg gctggcggac gatggtgttc acgacgctga cgctgtcgca gctggtgctc
     1321 gtgctgtcgc tgcgatccga gcgcgactcg ttcttcacga tcggcgcctt ctcgaacgcg
     1381 ccgctgctcg ccatcgtcgg gctgaccttc gcgctccaga tgatggtgac ctacgcgccg
     1441 ttctggcagc gcgtgctgaa gacgcacgcg ctgacggccg aggagctggg cgtcagcctc
     1501 gcgatggcga cggtgccctt cgccgccgac gaggtgcgga agtgggtgcg gcgcgcgcgg
     1561 cgggccagca aaggcggcgg agccgacgcg tcgccatgag gcggtaacag ggatgcgcac
     1621 cgagtatcac cgctggtact ccggccgggt cggccgggag atggggctgg tcgtctacgg
     1681 ccactggggc ccgccgatgc tggtgtttcc gaccagcggc ggcgacgagt gggagatgga
     1741 gcgccagggc atggtggcgg cgctggccga cttcatcgat cccggccggg tgaaggtgtt
     1801 caccatcgac gccaacagcc gcgacagctt ctacaaccgc ggcgcgcacc cgttccaccg
     1861 gagctacatg caggcgcagt acgacgcgta cgtccgcgag gaggtcgtgc ccttcatccg
     1921 gcggagctgc cagtcggacg acatcgccat cacgacggcg ggagcgtcgc tcggcgccta
     1981 ccatgccgcc aacaccctgt tcaaacaccc ggacgccgtc aggcgctgct acgcgctgtc
     2041 gggcgtgtac gacctgaagc gcttcatgga cggcatgtac gacgacaact tctacttcaa
     2101 caaccccgtg gactacatgt cgaacctcga cgacccgcgc ctgcgggagt ggctgggcgg
     2161 atgcgacatc cacctcgcga cggggcacgg gccgtgggag gacaaggggc cgacctaccg
     2221 catggcggag gtgctgagga cgaagggcat cccgcattcg ctcgacgact ggggcggcat
     2281 gggcggccac gactggccgt tctggaagca ccagatgcgc acgtacctgt ccgcctggtg
     2341 aagcaggccg acagggaggg
//
LOCUS       DAOSIF010000483         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_39442_length_2341_cov_3.009624, whole genome shotgun sequence.
ACCESSION   DAOSIF010000483 DAOSIF010000000
VERSION     DAOSIF010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39442_length_2341_cov_3.009624"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLT35_12835"
     CDS             <1..622
                     /locus_tag="PLT35_12835"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30157.1"
                     /translation="ALVLSGARTLPGWQQGDRAAMDFFDLKGVIEELGEALAVAGVEF
                     EPGERDGLRPGRSAAVRAGGEAIGWAGELHPTVAGRFGLEGRAVLAAELELDPLLRRA
                     AERFTVRPVPVYPPVKEDLAFVLDRAIPVSRVQEVIRAAGGPMLAGVTLFDEYAGEQV
                     GAGKRSLAFSLTYQAPDRTLTDADARKIRERVARALKDELGAALRA"
     gene            complement(662..1561)
                     /locus_tag="PLT35_12840"
     CDS             complement(662..1561)
                     /locus_tag="PLT35_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOG30158.1"
                     /translation="MRVVVIANPAAGPAWRRRHTGELARRVQAIFDRRGVQGDLSFTT
                     GPGHATSLARQAIADGADLVVAWGGDGTINEVAAPLVGSRSALGIVPVGSGNGLAREL
                     GIPRRPEAALAVALDGVDRTIDMGELNGRLFCNCAGVGFDAHVAEVFAASAARVRGFA
                     SYAATTIRELGAYQPGLYRIETDEGEEPPLRAMLITVANTRQWGNGALIAPNAVMDDD
                     RLDVVVIPPRGPAAFIANLWRLFAGSLASLEGVVNRQVRCATITASPAAPVHVDGEPL
                     GRLSTIRIEVKRAALRVRAPARA"
     gene            1728..>2341
                     /locus_tag="PLT35_12845"
     CDS             1728..>2341
                     /locus_tag="PLT35_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30159.1"
                     /translation="MKRTGWAAGFAVVAVALVGGQEREDRTLLSHGEMRAIINEASGD
                     RAVHAVYEMVPYPRVRDRAEYSTRFRESAVIAKLAQEFGFSDVAVESFAQGTPTWHAN
                     RAELWVAGPKPEKLYDVHDILVSVASGSDSGDVTAALVDVGTGTRPEDYAGKDVSGKI
                     VLGSGPASVLQRLAVFERGAVGVLSYNALRGDDYPDQLLSQSVSS"
BASE COUNT          315 a          827 c          894 g          305 t
ORIGIN      
        1 cgccctcgtg ctgtcagggg cgcgcacgct gccggggtgg cagcagggcg accgcgcggc
       61 catggatttc ttcgacctca agggcgtcat cgaggagctg ggagaggcgc tggccgtcgc
      121 cggcgtcgag ttcgagcccg gcgagcgcga cggcctgcgg ccgggccgct cggcggccgt
      181 gcgcgcgggg ggcgaggcca tcggctgggc cggggagctc cacccgaccg tcgccgggcg
      241 cttcggactc gagggccggg ccgtcctggc cgccgaactc gaactcgacc cgctgctgcg
      301 ccgcgcggcc gagcggttca ccgtgcgtcc ggtgcccgtc tacccgccgg tcaaggagga
      361 tctcgcgttc gtgctggacc gcgcgatccc cgtgtcgcgc gtgcaggagg tcatccgggc
      421 tgccggcggc cccatgctgg cgggcgtgac gctcttcgac gagtacgccg gcgagcaggt
      481 cggagccggc aagcgcagcc tggcgttcag cctgacgtac caggcgccgg atcgcacgtt
      541 gaccgacgcg gacgcccgga agatccgcga gcgcgtggcg cgcgcgctga aggacgagct
      601 cggcgcggca cttcgggcct gagtgcgaac ggggcgtcgg gcgcccgcag gcccgcccca
      661 gtcaggccct ggccggcgcg cgcacgcgca gcgcggcccg cttcacctcg atgcggatcg
      721 tcgagagccg cccgagcggc tccccgtcca cgtgcaccgg agcggccggc gacgccgtga
      781 tggtggcgca ccgcacctgg cggttcacga cgccctcgag cgacgcgagc gacccggcga
      841 agagccgcca caggttcgcg atgaaggcgg ccggcccgcg cggcggaatg acgaccacgt
      901 cgagccggtc gtcgtccatg acggcgttcg gcgcgatgag cgcgccgttc ccccactgcc
      961 gggtgttcgc caccgtgatc agcatggcgc gcagcggagg ctcctcgccc tcatcggtct
     1021 cgatgcggta gaggccgggc tggtacgcgc ccagttcccg gatggtggtg gcggcatagg
     1081 aggcgaaccc tcggactctc gccgccgagg cggcgaagac ctccgccacg tgcgcgtcga
     1141 atccgacgcc ggcgcagttg cagaacagcc gcccgttgag ttcgcccatg tcgatcgtgc
     1201 gatcgacgcc gtcgagcgcc accgccagcg ccgcctccgg ccgccggggg atcccgagct
     1261 cgcgtgcgag cccgttgccc gatccgacgg ggacgatgcc gagcgccgac cggctgccga
     1321 cgagcggggc ggcgacctcg ttgatcgtgc cgtccccgcc ccacgccacg acgaggtcgg
     1381 ccccgtcggc gatggcctgc cgggcaagag aggttgcgtg tcccgggccc gtcgtgaacg
     1441 agagatcgcc ctgcacgccg cggcggtcga agatggcctg gacgcgccgc gccagctccc
     1501 cggtgtggcg gcggcgccac gccggcccgg cggcggggtt cgcgatgacg acgactctca
     1561 tgggcacaga cagcctacca ccagcggcca gcgggcgcgc gagcaggggc cgcgggaggc
     1621 gccgggccgc cggtgccgga gggcggcttg cgcgcaagcc gccctccggc ctacccttca
     1681 taggtttgac ccgcgtctcc gagcagggag cgtgagaagg agtgagcatg aagaggactg
     1741 gatgggcggc ggggttcgcc gtggtggcgg tggcgctcgt cggcgggcag gagcgcgagg
     1801 atcgcacgct gctctcgcac ggcgagatgc gggccatcat caacgaagcg tccggcgatc
     1861 gggccgttca cgccgtgtac gagatggtcc cctatccccg cgttcgcgac cgggccgagt
     1921 attcgacccg cttccgcgag tccgctgtga ttgcgaagct cgcgcaggag ttcggcttca
     1981 gcgacgttgc ggtggagtcg ttcgcgcaag gcacgccgac gtggcacgca aaccgcgccg
     2041 agctgtgggt ggcggggccc aagcccgaga agctctacga cgtgcacgac atcctcgtgt
     2101 ccgtcgcgtc gggcagcgac tcgggtgacg tgacggcggc cctcgtcgac gtgggcaccg
     2161 gcacccggcc cgaggactac gccgggaagg acgtgagcgg gaagatcgtg ctgggaagcg
     2221 gaccggccag cgtgctccag cgcctggccg tgttcgagcg cggggccgtc ggcgtgctga
     2281 gctacaacgc gctgcgcggc gacgactacc ccgatcagct gctgtcccag agcgtgtcct
     2341 c
//
LOCUS       DAOSIF010000484         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_39704_length_2329_cov_3.655673, whole genome shotgun sequence.
ACCESSION   DAOSIF010000484 DAOSIF010000000
VERSION     DAOSIF010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39704_length_2329_cov_3.655673"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLT35_12850"
     CDS             <1..680
                     /locus_tag="PLT35_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOG30160.1"
                     /translation="VVARDGAMAVPPRPPSRTLRSTMNVAWESVDFRIPAAGRRARVI
                     GVKAGRLDTDALEAEPTVRGGEALADPSRDLLKIAVLERHMASGAAGKGFVTGIGLRE
                     GAIASSVAHDHHNLIVVGADDASMMTAARRVGALHGGMAVARGERVLAEVPLPLGGLM
                     SAEPVERVSRQVETAVAAARALGSPLHDPFMAMGFLALEVIPSLKVTDKGIIDVARRA
                     VVPLWLD"
     gene            874..1917
                     /locus_tag="PLT35_12855"
     CDS             874..1917
                     /locus_tag="PLT35_12855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOG30161.1"
                     /translation="MRHDHRACASAMTPPRPRVSILLPARNAAATIPACLASIRRQTE
                     PRWECIIVDDGSTDGTRDIAARAAEADGRFRVVSIAHAGLVSALNEGLRHCHGDLVAR
                     MDADDLMHRHRLEAQAACLDRNRDLAAVGCHVRMFPRAALSPRMREYEAWLNSLESAD
                     EVARDALVECPVAHPSLMMRRSMAGLGYVDRGWPEDYDLVLRALGRGLRIGVVPRRLL
                     AWRRSRRSLSRLDPRYARMQFTRCKAHFLAGGFLAARSDYVLWGFGETGKALHKALAA
                     LGKHPSHIVEVHPRRLGRRIHGAPVLPPEGMAGLRGRPAVVSVAREGPRSECRGMLAG
                     MGFTEGLDYVCTA"
     gene            complement(1973..>2329)
                     /locus_tag="PLT35_12860"
     CDS             complement(1973..>2329)
                     /locus_tag="PLT35_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30162.1"
                     /translation="IDPLTTLVIAAVILGSTWGLLRQSVNLALHAVPEWIDPAAVEAY
                     LSALPGVTAVHDLHIWAMSTTETALTAHLVKRDARDDDAIMAEATRVLHDRFGIHHTT
                     LQWERGAGACCPRLPV"
BASE COUNT          294 a          845 c          871 g          319 t
ORIGIN      
        1 cggtggtcgc gcgggacggc gcaatggcgg tcccgcctcg gccgccgtcg cgcacgctgc
       61 gcagcaccat gaacgtggcg tgggagagcg tggacttccg catccccgcc gccggccgcc
      121 gcgcgcgggt catcggcgtg aaagccggcc ggctggacac ggacgcgctc gaggccgagc
      181 cgaccgtacg cggcggcgag gcgctggccg atccgtcgcg cgacctgctc aagatcgccg
      241 tcctcgagcg ccacatggcg tccggggcgg ccggcaaggg cttcgtcacg ggcatcggcc
      301 tgcgcgaggg agccattgct tcgtcggtcg cgcacgatca tcacaacctg atcgtcgtgg
      361 gggccgacga cgcgtcgatg atgaccgccg cgcgccgcgt cggcgcgctg cacggcggca
      421 tggccgtcgc gcggggcgag cgggtcctcg cggaggtgcc gctgcccctc ggcggcctga
      481 tgagcgccga gcccgtggag cgcgtcagcc gccaggtgga gacggccgtg gccgcggcgc
      541 gcgcgctggg ctctccgctc cacgatccgt tcatggcgat gggctttctg gccctcgagg
      601 tgatcccgag cctcaaggtg accgacaagg ggatcatcga cgtggcgcgg cgcgccgtcg
      661 tgccgctgtg gctggactag ccggcgcgga cggccggggc ggcgccgcgc cgccgccgcg
      721 ccgacgaccc gctcccgaca ccgcggcagc ggaagcgcgg ccgcgggcct agcctgcggt
      781 cttccgggct cggcgagccg acgctcccgc ccgtgggcgt gcggtggagg acacgcggcg
      841 gcgcgcagga ggcctcgggg cttacactga agcgtgcgcc acgatcatcg cgcgtgcgcg
      901 tccgccatga cgccgccccg tcctcgagtg tccatcctcc tccccgcccg gaacgcggcc
      961 gcgacgatcc ccgcctgcct cgcgagcatc cgccgccaga ccgagccgcg ctgggagtgc
     1021 atcatcgtgg acgatggctc gacggacggg acccgcgaca tcgcggcccg cgcggcggag
     1081 gccgacgggc ggttccgggt tgtctcgatc gcgcacgccg gtctcgtttc cgccctcaac
     1141 gaagggctcc gtcactgcca tggcgacctc gtcgccagaa tggacgccga cgatctgatg
     1201 caccgccacc gcctggaggc ccaggcggcc tgcctcgacc gcaaccggga tctcgcggcc
     1261 gtcggctgtc acgtccgcat gttcccgcgc gcggcgctct cgccccgcat gcgggagtac
     1321 gaagcgtggc tgaacagcct cgaaagcgcc gacgaagtcg cccgcgacgc gcttgtcgag
     1381 tgcccggtcg cccatccctc gctcatgatg cgccggagca tggccggcct gggctacgtg
     1441 gatcgaggct ggccggagga ctacgacctg gtcctgcggg cgctcggccg aggcctgcgg
     1501 attggcgtcg tgcccaggcg cctgctggcg tggcgccgca gccggaggag tctgtcgcgt
     1561 ctcgatccca ggtacgcccg gatgcagttc acccggtgca aggcgcactt cctcgccggc
     1621 ggcttcctcg cggcccggtc cgattacgtg ctctggggct tcggtgaaac gggcaaagcg
     1681 ctgcacaagg cgctcgcggc gctcggcaag cacccctctc acatcgtcga ggtgcatccc
     1741 cggcgcctcg gccggcgcat ccacggcgcg ccggtcctgc cgccggaggg catggccggc
     1801 ctgcgcgggc gcccggccgt cgtgtccgtc gcccgcgagg gaccgcgatc ggagtgcagg
     1861 ggcatgctgg ccggcatggg attcaccgaa ggcctggact acgtctgtac cgcgtgagtg
     1921 gcccttgccg gagcgcgcgc ggtgtgcggc ctgccacgag cgggcggcgg cgtcagacgg
     1981 gcaggcgcgg gcagcaagcg cccgcgccgc gctcccactg gagcgtcgtg tggtggatgc
     2041 cgaagcgatc gtgcaggacg cgcgtcgcct cggccatgat cgcgtcatcg tcgcgcgcat
     2101 cccgcttgac cagatgcgcc gtgagcgcgg tctcggtcgt gctcatggcc cagatgtgca
     2161 ggtcgtgaac ggccgtcacg ccgggcagcg ccgacaggta ggcctccacg gctgccgggt
     2221 cgatccactc ggggacagca tgcagcgcga ggttgaccga ctggcgcagg agcccccagg
     2281 tgctgccgag gatgacggcg gcgatgacga gcgtcgtcag agggtcgat
//
LOCUS       DAOSIF010000485         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_40179_length_2307_cov_3.450266, whole genome shotgun sequence.
ACCESSION   DAOSIF010000485 DAOSIF010000000
VERSION     DAOSIF010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40179_length_2307_cov_3.450266"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..986
                     /locus_tag="PLT35_12865"
     CDS             141..986
                     /locus_tag="PLT35_12865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG30163.1"
                     /translation="MTFTRAVGSIFNVPLRGIIRVCIRLRVSPDVLTFCGLVLNVVAA
                     WYLAFGRFVTAGAWMAVANIFDYIDGKVAREGGVVTAFGAFFDSVMDRFSDITLFIGL
                     ISLYNNLGTGHRDYVLVTSLAMMFSVMTSYTRARAESLIETCKVGFMERPERIVLFMI
                     GAFTNRMAAVLWVILVLSIVTVLDRMYMTWRELKAGRSLAAVRAGTAPPPIPATQPGI
                     VTRAITLPALVLWRGLFWTYERGTWQYDVMVIVTLGFVLLTPPGWLHDPTAQFGASPL
                     QWLVR"
     gene            complement(1028..1585)
                     /locus_tag="PLT35_12870"
     CDS             complement(1028..1585)
                     /locus_tag="PLT35_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30164.1"
                     /translation="MRDPFGTLLILFGIGFLGANLALLYEGAQDWRRRRQAVLVWRMA
                     PPRLAFVPVLIAVGLAAVIVYKLVALQWPVWRLFGEAMMLTYYGYLYPLSQRVKRGFY
                     EEGVRLDRGFLRWEDINGLTWRPGPPLALLVARGRRQWAGVLTVPPEQFGATRRLLRD
                     RIAAREIRFSHPPLDLGGHDEREDV"
     gene            complement(1604..2191)
                     /locus_tag="PLT35_12875"
     CDS             complement(1604..2191)
                     /locus_tag="PLT35_12875"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOG30165.1"
                     /translation="MDIERTRHAGWIEVVCGSMFSGKSEELIRRLRRAQIARQKVQIF
                     KPQFDTRFADDEIVSHSEMRIPSQTVASSAELLQAVDEGTEVVGIDEGQFFDADLPAV
                     CNALADRGKRVIVAGLDQDYLGKPFEPMPQLLAVAEYITKTLAICMVCGNPANHTQRL
                     VASSDRLLLGAQGTYEARCRRCFDPTLSSGAEPSS"
BASE COUNT          330 a          830 c          781 g          366 t
ORIGIN      
        1 cccgcccgag gaacaggtac acgccgggct gctcgggcgc gtgggcgatc tggcgcttga
       61 ggtcgtggat cggcatggac tagaatcacc tgtccccggc ggagctggcg ctccgacgat
      121 tatacggacg ccccgaggag atgaccttca cccgcgccgt cggatcgatc ttcaacgtgc
      181 ccctgcgcgg gatcatccgc gtctgcatcc gcttgcgcgt ttccccggac gtcctcacct
      241 tctgcgggct cgtcctcaac gtcgtcgccg cgtggtacct ggccttcggc cggttcgtga
      301 ccgccggcgc ctggatggcg gtcgcgaaca tcttcgacta catcgacggc aaggtcgccc
      361 gcgagggcgg cgtcgtcacg gcgttcggcg ccttcttcga ctcggtgatg gaccggttct
      421 cggacatcac gctcttcatc ggcttgatct cgctctacaa caacctcggg acgggccatc
      481 gggactacgt cctcgtcacc tcgctggcca tgatgttctc ggtgatgacc agctacacca
      541 gggcccgggc cgagtcgctg atcgagacgt gcaaggtcgg cttcatggag cggcccgagc
      601 ggatcgtcct gttcatgatc ggggcgttca ccaaccgcat ggcggcggtc ctctgggtga
      661 ttctggtgct gtccatcgtg acggtgctcg accggatgta catgacgtgg cgcgaactga
      721 aggccggccg cagcctcgcc gccgtgcggg ccggcacggc gcccccgccc atccccgcca
      781 cccagcccgg catcgtcacg cgcgccatca cgctgccggc gctcgtcctg tggcgcggcc
      841 tcttctggac ctacgagcgc ggcacgtggc agtacgacgt gatggtgatc gtgacgctcg
      901 gcttcgtgct gctgacgccg ccgggttggc tgcacgaccc gacggcccag ttcggcgcca
      961 gccctctcca gtggctcgtt cgctgacgcc ccttcatccg ggattctgaa ttccggattc
     1021 cggattccta aacgtcctcc ctctcgtcgt gcccgcccag gtcgagcggc gggtgcgaga
     1081 agcggatctc gcgcgcggcg atgcggtccc gcagcaggcg ccgggtcgcc ccgaactgct
     1141 cgggcggcac cgtcagcacg cccgcccact gccggcggcc gcgcgccacg aggagcgcga
     1201 gcggaggccc cggccgccag gtcaggccgt tgatgtcctc ccaccgcagg aacccccggt
     1261 ccaggcgcac gccctcctcg tagaagccgc gcttcacccg ctgcgacagc gggtagaggt
     1321 agccgtagta ggtcagcatc atcgcctcgc cgaagaggcg ccagaccggc cactgcaggg
     1381 ccaccagctt gtagacgatg accgcggcca ggccgacggc gatgaggacc gggacgaacg
     1441 ccaggcgcgg aggcgccatg cgccagacga gcaccgcctg ccgccgccgg cgccagtcct
     1501 gcgcgccctc gtacaggagc gccaggttcg cgccgaggaa gccgatgccg aagaggatga
     1561 gcagggtgcc gaacgggtcc cgcacgccac ctcctgcggg ggcctacgac gagggctcgg
     1621 cgcccgacga cagggtcgga tcgaagcagc ggcggcaccg ggcctcgtac gtgccttgcg
     1681 ccccgaggag gagccggtcg ctgctcgcga cgaggcgctg cgtgtgattg gccggattgc
     1741 cgcacaccat gcagatggcc agcgtcttgg tgatgtactc cgcgaccgcc aggagctgcg
     1801 gcatcggctc gaacggcttc ccgaggtagt cctgatcgag gccggccacg atgacgcgct
     1861 tgccccggtc ggccagcgcg ttgcagacgg ctggcagatc ggcgtcgaag aactgccctt
     1921 cgtcgatccc cacgacctcg gtgccctcgt cgacggcctg gagcagctcc gccgaggagg
     1981 cgacggtctg cgaggggatg cgcatttcgc tgtgcgacac gatctcgtcg tcggcgaagc
     2041 gcgtgtcgaa ctggggcttg aagatctgca ccttctggcg cgcgatctgg gcccggcgga
     2101 ggcggcggat cagctcctcg ctcttgccgc tgaacatgct gccgcacacg acctcgatcc
     2161 acccggcgtg ccgcgtccgc tcgatgtcca tgtcaatacg ccgaggctgc gatcccggcc
     2221 tccatcagcc acccccgagc ggcaggcccc cgtcctcgag ccccgctcgc ccgccgcggc
     2281 cggcgggcgg gggatccggc ggaggcg
//
LOCUS       DAOSIF010000486         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_40844_length_2279_cov_3.796763, whole genome shotgun sequence.
ACCESSION   DAOSIF010000486 DAOSIF010000000
VERSION     DAOSIF010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40844_length_2279_cov_3.796763"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /locus_tag="PLT35_12880"
     CDS             complement(<1..2104)
                     /locus_tag="PLT35_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG30166.1"
                     /translation="MAGRLQQNRTRLQFSYNLGGSPFNARPYAINGQSPNEPSNLQHR
                     FGVNIGGPLKVPGLFDAGPRANYVLNYSGSRGRTFRESYSRVPTSAQRSGDLSSSTGV
                     IHDPLTGAPFEGNRIPADRISPAAASLLALYPLPNQDTAGQNYHFATTVVSHSDDVNV
                     RLTRSFGTAQAGRGGGPGGGRGGMMGGPGGRGGGSVNVSLGVQFRRSSGDQATSFPAL
                     AGKNAQTGWNVPASVVFQRWGLFNSVNVQYNRSRATTTNRFANVRDVAGEAGIAGVST
                     DPFSWGAPSLSFTTFSGLRDITPSERTDQTITLSLSQSRMRGRHTLRWGADVRWMRND
                     SRSDSNPRGSFVFTGLYSQRTKIAGAGNDFADFLLGLPQQASLQYGPGTLRFRSSAWS
                     AFVQDDWRVRANLTINAGVRYEYLSPYREANNHLVNLDVAPDFTAAVPVLAGQAGPFT
                     GRYPATIVEPDWNNAAPRIGVAWRPRPRLVVRAGYGISYSSPVYQGMARQLAAQPPFA
                     VTDTRIGTLAAPLSMAAVFTWPGADQTTNNFGVDREYDLGHLQMWNAGVTRDLTRTLS
                     VGLTYVGTKGGSLDLLRAPNRGPEGLLIDGVQAFTWESSGGRSIMHSLSMQLRRRPAR
                     GVGGGLTYTWSKAMDNASSLGGGGGGSGVVAQNDKDLEAEWGLSGFDQRHRLQAEFSV
                     ELPFGPNRRWLNNDGWAAK"
BASE COUNT          313 a          797 c          828 g          341 t
ORIGIN      
        1 ccttcgccgc ccagccgtcg ttgttcagcc agcgccggtt cggcccgaag ggcagctcga
       61 cgctgaactc ggcctggagc cggtggcgct ggtcgaagcc ggacaggccc cattccgcct
      121 cgaggtcctt gtcgttctgc gcgactacgc ccgagccgcc tccgccgccc ccgagcgacg
      181 aggcgttgtc catcgccttc gaccacgtgt acgtgagccc gcccccgacg cccctcgcgg
      241 ggcgcctgcg cagctgcatc gacagggagt gcatgatcga gcggccgccc gaggattccc
      301 acgtgaacgc ctgcacgccg tcgatgagca ggccttccgg cccccggttc ggcgcgcgca
      361 gcaggtccag gctgccgccc ttcgtgccga cgtaggtgag gccaacgctg agcgtgcgcg
      421 tcagatcccg cgtgacgccg gcgttccaca tctgcaggtg cccgaggtcg tactcgcggt
      481 ccacgccgaa gttgttcgtg gtctggtcgg ccccgggcca ggtgaagacc gccgccatcg
      541 agagcggggc ggcgagggtg ccgatgcgcg tgtcggtcac ggcgaagggc ggctgggccg
      601 ccaactggcg ggccatgccc tggtacacgg gcgagctgta gctgatgccg tagcccgcgc
      661 gcacgacgag ccggggcctc ggccgccagg cgacgccgat ccggggcgcg gcgttgttcc
      721 agtcgggctc gacgatggtc gccgggtaac ggcccgtgaa ggggcctgcc tgtccggcga
      781 gcaccgggac cgcggccgtg aagtccggcg cgacgtcgag attgaccagg tggttgttcg
      841 cctcgcggta cggcgacagg tactcgtagc ggacgcccgc gttgatcgtc aggttcgcgc
      901 gaacccgcca gtcgtcctgc acgaacgcgc tccacgccga cgaccggaac cggagcgtcc
      961 ctgggccgta ctgcagcgac gcctgctggg gcaggccgag caggaaatcg gcgaaatcgt
     1021 tgcccgcccc cgcgatcttc gtccgctgcg agtagagccc ggtgaacacg aagctgccgc
     1081 gcgggttcga gtccgaccga ctgtcgttcc gcatccaccg gacgtcggcg ccccagcgca
     1141 gcgtgtggcg cccgcgcatc ctcgactgcg acaggctgag cgtgatcgtc tggtccgtcc
     1201 gctccgaggg cgtgatgtcg cgcaggccgc tgaacgtggt gaacgagagc gacggcgcgc
     1261 cccaggagaa cgggtcggtc gagacgcccg cgatgccggc ctcgcccgcc acgtcgcgca
     1321 cgttcgcgaa gcggttcgtc gtcgtggcgc ggctgcggtt gtactgcacg ttgaccgagt
     1381 tgaagagccc ccagcgctgg aacaccacgc tggccggcac gttccagccc gtttgcgcgt
     1441 tcttgccggc cagcgcgggg aagctcgtcg cctggtcgcc ggacgagcgg cggaactgca
     1501 cgccgagcga cacgttgacc gagcctccgc ccctgccgcc cggcccgccc atcatgccgc
     1561 cccggccgcc tcccggcccg cccccgcgcc cggcctgcgc cgtgccgaag gaccgcgtca
     1621 ggcggacgtt gacatcgtcg gaatggctca caaccgtcgt ggcgaagtgg tagttctggc
     1681 ccgccgtgtc ctggttgggc agcgggtaga gcgcgaggag cgacgcggct gccggactga
     1741 tgcgatcggc ggggatccgg ttcccctcga acggcgcgcc ggtcagcgga tcgtgtatga
     1801 cgcccgtgct cgatgagagg tctcccgagc gttgcgccga ggtcggcacc cgcgagtagg
     1861 actcgcggaa cgtccgcccg cggctgccgc tgtagttcag gacgtagttc gcccgcgggc
     1921 ctgcgtcgaa caggccgggg accttgagcg ggccgccgat gttcacgccg aaccggtgct
     1981 ggagattgga cggctcgttg ggagactgcc cgttgatcgc gtagggccgc gcgttgaagg
     2041 gagagccgcc gaggttgtac gagaactgga ggcgggtgcg gttctgctgg agccggccgg
     2101 ccatctgcag gcggccagcc gcgcctgcat cagcgcgcag tattgagggt tcaggtcgtt
     2161 caggatcgcg gagcgtcccg cgtctctggc cgccatgccg gtctgccctg tgccagccat
     2221 cggatccagg acgatgcagg gccgacgggg ccaggatgcc aggacgcgcg cgtcccacg
//
LOCUS       DAOSIF010000487         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_40875_length_2278_cov_2.435897, whole genome shotgun sequence.
ACCESSION   DAOSIF010000487 DAOSIF010000000
VERSION     DAOSIF010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40875_length_2278_cov_2.435897"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PLT35_12885"
     CDS             <1..1019
                     /locus_tag="PLT35_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG30167.1"
                     /translation="LLNYARVVGITRAADGFVDGVVAVDEESGREIRAGAKVVINATG
                     PFADGVRRLANPSAASLIAPSQGVHLVFDGSFLPGRSAIMVPHTRDGRVMFAIPWHGH
                     TLVGTTDTPIAEPVLEPRAFDQEVAFIIETASQYLHRAPTRADVLSVSVGIRPLVRAE
                     GASSTAALSRDHTIHIDQSGLLTVAGGKWTTYRHMAEDAVNQAAMLARLPEQACRTRT
                     LNIHGFHPHAEKFGALSVYGSDALAIEDMMRAHPPLAQSLHGALPYRRAEVVWAVRVE
                     MARSVEDVLARRTRALILNAAAAVEMAPEVARLMAAELRMDDAWQRGQVAAFAELAQS
                     YSLS"
     gene            complement(1066..>2278)
                     /gene="pgtP"
                     /locus_tag="PLT35_12890"
     CDS             complement(1066..>2278)
                     /gene="pgtP"
                     /locus_tag="PLT35_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate transporter protein PgtP"
                     /protein_id="HOG30168.1"
                     /translation="VRNTFALAIPDVLKEHPEYSKAALGTAMTGLSIAYGLSKFLMGS
                     VSDRSNPRYFLPLGLLLSAGIMFLSGAVQAIYASLVIVVLLQTLNGWVQGMGWPPCGK
                     TMVHWFSTNERGIVVSVWNTAHNVGGALVATFALGGLAIFGHWGAKLYFNAMIAAVVA
                     IVAFLLMRDTPQSCGLPPIEVHRNDYPPDYSEAHERNFTFREIFFGHVLTNKYLWAIA
                     VANAFVYFVRYGVVNWIPTYLETAKGFSFKDASWAWALYEWAAIPGTIACGWVSDKVF
                     KSRRAPATILFMALTLVAVIVYWLNMRGPLWIDYAALIAVGFLIYGPIMIIGLHALDL
                     VPKKAAGTAAGFTGFFGYVFGSAIAGTGVGWIADEWGWNGVFVTMVVCCVLTMAFSAL
                     TLAHSAVSEKR"
BASE COUNT          376 a          799 c          780 g          323 t
ORIGIN      
        1 cgctgctgaa ctacgcccgg gtcgtcggca tcacccgggc cgccgacggg ttcgtggacg
       61 gggtcgtggc cgtcgacgag gagtcgggcc gggagatccg ggcgggcgcc aaggtcgtca
      121 tcaacgccac cgggccgttc gccgacggcg tgcgccggct ggcgaacccg tcggccgcga
      181 gcctcatcgc cccgagccag ggcgtgcacc tcgtcttcga cgggtcgttc ctgcccgggc
      241 ggagcgctat catggtgccc cacacgcgcg acggccgcgt catgttcgcc atcccttggc
      301 acggccacac gctcgtcggc acgaccgaca cgccgattgc cgagcccgtg ctcgagccgc
      361 gggcgttcga tcaggaggtg gcgttcatca tcgagacagc gtcgcagtac ctgcaccgcg
      421 ccccgacgag ggccgacgtg ctgagcgtct cggtgggcat ccggccgctc gtgcgcgccg
      481 agggcgcctc gtcgacggcc gccctgtcgc gcgaccatac gattcacatc gaccagtcgg
      541 gcctgttgac ggtcgccggc ggcaagtgga ccacgtaccg gcacatggcc gaggatgccg
      601 tgaaccaggc ggcgatgctc gcccggcttc ccgagcaggc gtgcaggacg cgcacgctga
      661 acatccacgg gttccatccg cacgccgaga agttcggcgc gctgagcgtc tacgggtcgg
      721 acgccctcgc gatcgaggac atgatgcgcg cacacccgcc gctcgcccag tcgctgcacg
      781 gcgcgctccc ctaccggcga gccgaggtcg tctgggccgt gcgggtggag atggcgcgct
      841 ccgtcgagga cgtgctggcc aggcgcacgc gcgcgctcat cctcaacgcc gccgcggccg
      901 tcgagatggc gcccgaagtg gcgcgcctga tggccgcgga actccggatg gacgacgcgt
      961 ggcaacgcgg ccaggtggcc gccttcgcgg agctggcgca gagctacagc ctgtcgtagg
     1021 ggcgccggcc gcccccgctc ccggggccgc gccgcggcgc ggcccctacc gcttctcgct
     1081 gacggcgctg tgcgccagcg tcagcgcgct gaaggccatc gtcaagacgc agcagacgac
     1141 catcgtcacg aagacgccgt tccagcccca ttcgtccgca atccagccga cgcccgtgcc
     1201 ggcgatggcg gatccgaaca cgtagccgaa gaaccccgtg aacccggcgg ccgtgccggc
     1261 ggccttcttc ggcaccagat ccagcgcgtg cagaccgatg atcatgatcg gcccgtagat
     1321 caggaagccg accgcgatga gcgcggcgta gtcgatccac agcggaccgc gcatgttcag
     1381 ccagtacaca atcacggcaa ccagcgtcag agccatgaag aggatcgtgg ccggcgccct
     1441 ccggctcttg aagaccttgt ccgagaccca cccgcacgca atcgtccccg ggatggccgc
     1501 ccactcgtac agcgcccagg cccagctcgc gtccttgaac gagaagcctt tcgccgtctc
     1561 caggtaggtc gggatccagt tcacgacgcc gtaccggacg aagtagacga aggcgttcgc
     1621 gaccgcgatg gcccagaggt acttgttggt gagcacgtgc ccgaagaaga tctccctgaa
     1681 cgtgaagttg cgctcgtggg cctcgctgta gtccggcggg tagtcgttcc ggtggacctc
     1741 gatcggcggc agcccgcacg actgcggcgt gtcgcgcatg aggaggaacg ccacgatggc
     1801 caccaccgcg gcaatcatcg cgttgaagta gagcttcgcg ccccaatggc cgaagatcgc
     1861 gagccccccc agggcgaagg tggcgacgag tgcgcccccg acgttgtgcg cggtgttcca
     1921 gacggacacg acgatgccgc gctcgttcgt gctgaaccag tgcaccatcg tcttgccgca
     1981 cggcggccag cccatgccct ggacccagcc gttcagcgtc tgcagcagga cgacgatcac
     2041 gagagaggcg tagatcgcct gcacggcccc gctgaggaac atgatgcccg ccgagagcag
     2101 gaggccgagc ggcaggaagt aacgcgggtt gctgcgatcg gacaccgatc ccatcaggaa
     2161 cttggacagc ccgtaggcaa tcgagagccc cgtcatcgcg gtgccgagag cggccttgga
     2221 gtactccggg tgctccttga ggacgtcggg gattgccagc gcgaaggtgt tgcggacg
//
LOCUS       DAOSIF010000488         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41069_length_2270_cov_2.479007, whole genome shotgun sequence.
ACCESSION   DAOSIF010000488 DAOSIF010000000
VERSION     DAOSIF010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41069_length_2270_cov_2.479007"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLT35_12895"
     CDS             <1..852
                     /locus_tag="PLT35_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30169.1"
                     /translation="FSVGAAHAREFEAAMLRAAELLAESRVASQREQAAGWRLLRATE
                     VADGAPLSYVLWCDPAVKGIDYSMRAVAALAEAGEARRLEDVVQLSQAGPAVQAEYRV
                     VMAMRADASASPAAAAGAPGAADEPATQAPGSPAQRGRTFSGDVGVQFNFVKADHVEA
                     FEASMTTLRGALAASTNPVRRAQADSWRVLRQTAGEPAGQAVYLYLVDPIVTGADYAP
                     SAIVREALGPEEARAFAATYSTAFARGVSLLGFTLVKPGMVDSPDVRRAGTDTGSPVG
                     RLPRVLR"
     gene            935..1342
                     /gene="mce"
                     /locus_tag="PLT35_12900"
     CDS             935..1342
                     /gene="mce"
                     /locus_tag="PLT35_12900"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOG30170.1"
                     /translation="MKATLDHVGIAVADLGKALAFYRDALGLEIGAPEEVPSQRVRVH
                     FVPAGEAALELLEPTSPESPIAKFIEKRGPGVHHLTLRVDDIAAALARLKANGVRLID
                     ESPRPGAEGALIAFVHPASTHGVLVELKQRAEG"
     gene            1346..2206
                     /gene="mtnP"
                     /locus_tag="PLT35_12905"
     CDS             1346..2206
                     /gene="mtnP"
                     /locus_tag="PLT35_12905"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOG30171.1"
                     /translation="MTCEIGIIGGSGLYDMPELTDRAEARLDTPFGEPSGPYLTGTLR
                     GRRVAFLARHGAGHRLSPSELNSRANIFGFKLLGVERILSASAVGSLREDYRPQDIVI
                     PDQFVDRTKGRASSFFGGGLVAHIGFAHPICAEAAAVAAAAGRAVGARVHDRGTYVCM
                     EGPAFSTLAESRAYRAMGFDIIGMTNLPEAKLAREAELCYASIALVTDYDCWHPDHDS
                     VTVDMVVGNLRANADTARRLIAEAVGRMPSSRGCACGRALEHAILTQPAAVPAEVRRA
                     LAPLVAKYLP"
BASE COUNT          299 a          811 c          842 g          318 t
ORIGIN      
        1 ttctccgtcg gcgctgctca tgcccgggag ttcgaggcgg cgatgctgcg ggccgccgaa
       61 ctgctggcgg agagccgcgt ggcgagccag cgcgagcagg cggccggctg gcggctcctg
      121 cgggcgaccg aggttgccga cggcgcgccg ctgagctatg tgctctggtg cgatcccgcc
      181 gtgaagggca tcgactactc gatgcgagcc gtcgcggccc tcgccgaggc cggggaagcg
      241 cgccgcctcg aggatgtcgt ccagctctcg caggccggcc ccgcggtcca ggccgagtac
      301 cgcgtggtga tggcgatgcg cgccgacgcc agcgcctcgc ccgcagcggc agccggcgcg
      361 cctggtgcgg ccgacgagcc ggccacgcag gcgcccgggt cgcccgcgca gcgcggccgg
      421 accttcagcg gcgacgtcgg cgtgcagttc aacttcgtca aggcggacca cgtcgaggcg
      481 ttcgaggcgt cgatgacgac gctgcggggc gccttggcgg ccagcacgaa tccggttcgg
      541 cgtgcccagg ccgacagctg gcgcgtcctg cggcagacgg ccggcgagcc ggcaggccag
      601 gcggtctacc tctacctggt ggatccgatc gtcaccggag ccgattatgc gccctcggcc
      661 atcgtgcgcg aggcgctcgg gcccgaagag gcccgggcgt tcgcggcgac gtacagcacg
      721 gcgtttgcgc gcggcgtgtc gctgctcggc tttaccctcg tgaagcccgg catggtagat
      781 tcccctgacg tgcgccgcgc cgggacggac acggggagcc ccgtcggccg cctgccgcgc
      841 gtgctccgct gacgacccgg cgccctgcgg ggcgcgaccc cgggcggcag gtcgcgcggc
      901 cgccgagccc gggcatcctg gaggagtgac acccgtgaaa gccacgctcg atcacgtcgg
      961 aatcgccgtt gccgacctcg gcaaggcgct ggcgttctac cgcgacgcgc tcggactgga
     1021 gatcggcgcg ccggaagaag tgccgtcgca gcgcgtgcgc gtgcacttcg tgccggcggg
     1081 agaggcggca ctcgagctgc tcgaaccgac gtcgcccgag tccccgatcg cgaagttcat
     1141 cgagaagcgc ggcccgggcg tgcaccactt gacgctccgg gtggacgaca tcgccgcggc
     1201 gctggcgcgc ctcaaggcga acggcgtgcg gctgattgac gagagcccaa ggccgggggc
     1261 cgagggcgcg ctcatcgcct tcgtccaccc cgccagcacg catggcgtcc tggtcgaact
     1321 gaagcagcgc gcggagggct gagcggtgac gtgtgagatc ggcatcatcg gcgggtccgg
     1381 cctgtacgac atgccggagc tgacggaccg ggcggaggcg aggctcgaca ctccgttcgg
     1441 cgagccttcg ggcccctacc tgaccggcac gctccgcggg cggcgggtgg cgtttctcgc
     1501 gcggcacggc gcgggccacc gcctctcgcc atccgagctg aactcgcgcg cgaacatctt
     1561 cgggttcaag ctgctcggcg tcgagcggat tctctcggcg agcgccgtcg gcagcctccg
     1621 cgaggactac cggccgcagg acatcgtgat cccggaccag ttcgtcgacc gcacgaaggg
     1681 ccgggccagc tccttcttcg gcgggggcct cgtggcgcac atcggcttcg cgcacccaat
     1741 ctgcgccgag gccgcggccg tcgcggcagc ggcgggccgc gccgtcggcg cgcgcgtgca
     1801 cgatcgcggc acgtacgttt gcatggaggg cccggcgttc tcgacgctgg ccgagtccag
     1861 ggcctaccgc gccatgggct tcgacatcat cggcatgacg aacctgcccg aggcgaagct
     1921 cgcgcgcgag gccgagctgt gctacgcgag catcgccctt gtgacggact acgactgctg
     1981 gcacccggat cacgactcgg tcaccgtcga catggtcgtc ggcaatctcc gggcgaacgc
     2041 ggacactgcg cggcggctca tcgccgaggc ggtgggccgc atgccgtcct cgcgcgggtg
     2101 cgcgtgcgga cgggcgctcg agcatgcgat cctcacccag cccgcggccg ttccggccga
     2161 ggtcaggcgg gcgctggcgc cgctcgtcgc caagtacctc ccctgatgat gcgcatcatc
     2221 gtcaccggct cgatcgcgac cggtggcatg gtcggctttt tcatcagcgg
//
LOCUS       DAOSIF010000489         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41208_length_2264_cov_3.053418, whole genome shotgun sequence.
ACCESSION   DAOSIF010000489 DAOSIF010000000
VERSION     DAOSIF010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41208_length_2264_cov_3.053418"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLT35_12910"
     CDS             <1..275
                     /locus_tag="PLT35_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30172.1"
                     /translation="IEATFGRRSYSGLGKAEQFIGLVIFAAALASAPALSIRVGGARL
                     SAANVLLGLLGAGALAALAHRLGLGWSLARQGAGPAANRAPKVPPR"
     gene            272..1189
                     /locus_tag="PLT35_12915"
     CDS             272..1189
                     /locus_tag="PLT35_12915"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG30173.1"
                     /translation="MTLSEIGFQALLRWLLFPAAAIPVCVYARVHGHHGFLERLGPWF
                     LIIPVALGASYLGMVPFGLLLAMCGAVAAWELARLGQALPAPRSGAAAVLGARPSRFA
                     LALACVLPWILWPALGPSDSAIVAGAAVLLPVLLFFALPRAAAAVWTVPLLPLSLGAA
                     LSFWVRLQQMPAGFRWVLVAFSVVVVNDVMGFVTGRLLPGPRPFPSLSPGKTITGYAG
                     GLAGAVLTAFVLAFAEPEFGALTIAAAAVALACSGAAGDLLASAVKRRHQAKDFGRAL
                     GAQGGMLDRLDSLLGAGWIFYLFVTLIAR"
     gene            1186..>2264
                     /locus_tag="PLT35_12920"
     CDS             1186..>2264
                     /locus_tag="PLT35_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30174.1"
                     /translation="MRTRRMAAPAWRSETVSASLFALPRLATSFVATGIVTLAVPQMV
                     VLAVGEARKGLHLGSISAAASIASLAALYLSGMHSDRRRLDGGRRHLPVVWLAVMALP
                     LAALASGSSYPAAIAAIMALVVTRSLCDAAHLPIIADDFHGGLAGEPGVPRPGVLSAH
                     IAFQQFLGAALGALAFAWLPVTGGGPAGLRTASAAVAIALLVLAAAGFLLSFRRLPRL
                     ALGTRGAREAEPAPSSSGQRGVRTGSAHDGSADGAVITPNLRALLAARTLFMAGVFII
                     STFLVFLVRDVLAAPDVERVTAILVGGTLSGALLSALPAGRLASTFGDRRVLFAAGAA
                     VAAVAPAFLAFAPGRPAFALLCMLL"
BASE COUNT          203 a          827 c          826 g          408 t
ORIGIN      
        1 agatcgaagc cacgttcggg cgccgctcct acagcggcct gggcaaggcc gagcagttca
       61 tcggcctcgt catcttcgcc gcggcgctgg cctcggcgcc cgcgctgtcc atccgcgtgg
      121 gcggagcgcg gctgtcggct gcgaacgttc tgttgggcct gctgggagcc ggcgccctgg
      181 ccgcactcgc gcaccgcctg gggctcggat ggtcgctggc gcgccagggc gcagggccgg
      241 cggccaatcg cgcgcccaag gtcccgccac ggtgacgctc tccgagatcg gtttccaggc
      301 gctcctgcgg tggctgctgt tcccagccgc tgccattcca gtctgcgtgt acgcccgcgt
      361 ccacggccac catggattcc tggagcgcct gggaccctgg ttcctgatca tcccggtggc
      421 gctgggcgcg tcttacctcg gcatggttcc cttcggactg ctcctggcga tgtgcggcgc
      481 ggtcgccgcg tgggaactcg ccaggctggg gcaggcgctg ccggcgcccc gatccggcgc
      541 cgcagccgtg ctgggtgcca ggccgtcgcg cttcgccctt gcactggcct gcgtgctgcc
      601 gtggatcctc tggcccgcgc tcggcccgag cgattccgcg atcgtcgccg gcgccgccgt
      661 gctcctcccg gtgctgctgt tcttcgcgct gccgcgggca gcggcggcgg tctggacggt
      721 cccgctgctg ccgctctcgc tcggggcggc gctctcgttc tgggtgcggc tgcagcagat
      781 gccggcgggg ttccgctggg tgctcgtcgc cttcagcgtc gttgtcgtga acgatgtgat
      841 gggtttcgtc acgggccggc tcctgccggg accgcgtccg tttccgtcgc tcagcccggg
      901 aaagacgatt actggctacg ccggcggctt ggcgggcgcc gtgctgaccg ccttcgtcct
      961 cgccttcgcg gagccggagt tcggtgcgct gacgattgcc gccgcggcgg tcgcgctcgc
     1021 gtgcagcgga gcggccgggg atctgctcgc ctcggcggtg aagcgacgcc accaggccaa
     1081 ggacttcggc cgcgcgctcg gcgcgcaggg cggcatgctc gaccgcctgg actcgctgct
     1141 tggcgccggc tggatcttct acctgttcgt cacgctcatc gcgcgatgag gacacgcagg
     1201 atggccgcgc ccgcgtggcg ctccgagacg gtcagcgcgt ccctgttcgc tctgcctcgc
     1261 ctcgccacgt ccttcgttgc cacgggcatc gtcacgctcg cagtgcccca gatggtcgtg
     1321 ctggccgttg gggaggccag aaaggggctc catctcggga gcatcagcgc cgccgcgtcc
     1381 atcgccagcc tcgcggcgct gtacctgtcc ggcatgcact ccgaccgccg gcgcctggac
     1441 ggcgggcgcc ggcacctgcc agtggtctgg ctcgccgtga tggccctccc gctggcggcg
     1501 ctggcctcgg ggtcctccta tccggcggcc atcgctgcga tcatggccct ggtcgtgacg
     1561 cggtcgctgt gcgacgcagc gcacctgccc atcatcgccg atgacttcca cggcggactg
     1621 gccggcgagc caggggtgcc gcgtcccggc gtgctgtcgg cgcacatcgc gtttcagcag
     1681 ttcctcggcg cggcgcttgg agccctggcg tttgcgtggc tgccggtgac gggaggaggg
     1741 ccggcagggc ttcggacggc gtcggcggcc gtcgccatcg cgttgctggt cctcgccgca
     1801 gccgggttcc tgctctcgtt ccgccggttg cctcgtctcg cactagggac tcggggcgcg
     1861 agggaagcgg agcctgcgcc gtcctcgagc gggcagcggg gtgtgcggac gggctccgcg
     1921 cacgacggca gcgcagatgg cgccgtgatc acgccgaacc tgcgcgccct gctcgccgcg
     1981 cgcacgctct tcatggcggg cgtgttcatc atctcgactt tcctcgtctt cctggtccgc
     2041 gacgtgctgg cggctcccga cgtcgagcgg gtcacggcga tcctcgtcgg ggggacgctc
     2101 tcaggggcgc tgctgagcgc gctgcccgcc ggccgcctgg cctcgacgtt tggggaccgg
     2161 cgggtgctgt ttgcggcggg ggccgccgtg gcggccgtcg cgccggcgtt cctggccttc
     2221 gcgccgggcc gccccgcctt cgccctgctg tgcatgctcc tcta
//
LOCUS       DAOSIF010000490         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41226_length_2263_cov_3.711504, whole genome shotgun sequence.
ACCESSION   DAOSIF010000490 DAOSIF010000000
VERSION     DAOSIF010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41226_length_2263_cov_3.711504"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLT35_12925"
     CDS             <1..524
                     /locus_tag="PLT35_12925"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG30175.1"
                     /translation="WAWPGSSCTARFSRMMAFSGAGSVVGAIVVAWLGRFSAMARTAV
                     RLQAFLGVLLVAFAASRVLALSYGLLFLGSLAMMMVFSLLTSLVQLEAPDQMRGRVMS
                     IYTMAFRGGAPLGSIAAGALAARTSAPAALAATGALLTLIVGAFAWRSASHWEPAPPV
                     PDRDGQSGGSQTQ"
     gene            complement(497..2038)
                     /locus_tag="PLT35_12930"
     CDS             complement(497..2038)
                     /locus_tag="PLT35_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30176.1"
                     /translation="MPQHTAPAVVRREGGGSTREFAAAAVLVVSVAVLRGVAFLVWEH
                     ASFDSDQAVTGLMAKHLIEGRAFPLFYYGQNYLLAVQAWLAAPVFLATGPTVAALKLP
                     LVLLNATTALVLLRVLSHDLGLRPWLAVMASAFFVAAPPGTAAQLVSALGASVEPFLY
                     VLLIWLLRDRPLWQGAVLGVGFLQREFTAYGFIAFVAVLAFDGTLFSRARAAKIAAAL
                     GAAIAVWLAVAALRPFSSGAGPGTTPLDVAGAANNLRGLLDRVCTDPSRIASGFGTLL
                     GSYLGLPLGLTPARLSGFLVNSRLYQGLTWAWPVFAAGCLAGLVRAAVLARRRGVRAT
                     VRDAGAAIYLVVVGALAAAGYTVGRCGDLQIETVRYVLLALLMPVGLAGAWLRLEPRP
                     AIRAAIVGVIAAWSAVSVAGHARLVNEYVRDRPPAYRRALTDYLVQRRIRLVRTDYWT
                     GYQVAFLSQERVVADTDGVWRVLQYHRWAEQRPHAVWYVGRGPCANRGEEAVPGVYWV
                     CDPPD"
BASE COUNT          335 a          843 c          815 g          270 t
ORIGIN      
        1 catgggcgtg gccggggtcc agttgcacgg cacgcttcag ccggatgatg gccttctccg
       61 gtgcgggctc ggtggtgggg gcgatcgtcg tcgcgtggct gggacgcttc tccgccatgg
      121 ctcgcacggc ggtccggctg caggccttcc tcggcgtcct gctcgtggct ttcgccgcgt
      181 cgcgcgtcct ggcgctgagc tacgggctgc tcttcctggg cagcctcgcg atgatgatgg
      241 tcttctcgct gctcacctcg ctcgtgcagc tcgaggcccc tgaccagatg cgcggccgcg
      301 tcatgagcat ctacacgatg gccttccggg gcggcgcgcc cctcgggtcc atcgccgccg
      361 gcgccctcgc ggcccgcacc tccgcaccgg ccgccctcgc agcgaccggg gcgctgctga
      421 cgttgatcgt cggcgccttc gcgtggcgtt cggcgtcgca ctgggagccg gcgccgcccg
      481 ttcccgaccg cgacggtcag tcgggcggat cgcagaccca gtagacgccg ggcacggcct
      541 cctcgccgcg attggcgcac ggccctcgcc cgacgtacca gaccgcgtgc gggcgttgct
      601 cggcccacct gtggtactgc agcacgcgcc acacgccgtc cgtgtcggcc acgacccgct
      661 cctgcgacag gaacgccacc tggtatccgg tccagtagtc ggtccgaacg agccggatgc
      721 ggcgctgcac gaggtagtcg gtcagcgccc ggcgataggc gggcggcctg tcgcgcacgt
      781 actcgttcac cagccgcgcg tgcccggcga cgctgacggc cgaccaggcg gcgatgacgc
      841 cgacgatggc cgcgcggatg gccgggcggg gctcgagccg cagccacgcg ccggcgaggc
      901 cgacgggcat caggagggcg agcagcacgt acctcaccgt ctcgatctgg aggtccccgc
      961 agcgtccgac cgtgtacccg gccgcggcaa gcgctccgac gacgacgagg tagatggcgg
     1021 cgccagcgtc gcgcaccgtc gcgcggaccc ctcgacgccg cgcgagcacc gcggcgcgaa
     1081 cgaggcccgc caggcacccg gcggcgaaca cgggccatgc ccaggtcaag ccctggtaga
     1141 gccggctgtt gacgaggaac ccggacaagc gcgccggcgt caggcccagc ggcaggccga
     1201 gatacgagcc gaggagagtg ccgaagccgc tcgcgatccg cgacgggtcc gtgcacacac
     1261 gatcgagaag cccgcgcagg ttgttcgcgg cgcccgcgac gtcgagcggc gtcgtccccg
     1321 ggcctgcgcc gctcgagaag ggacgcagcg ccgcgacggc cagccagacc gcaatcgcgg
     1381 cgcccagcgc ggccgcgatc ttggccgcgc gcgcgcggct gaagagcgtt ccgtcgaagg
     1441 ccagcacggc gacgaacgcg atgaacccgt aggcggtgaa ctcgcgctgc aggaacccga
     1501 ctcccaacac ggccccctgc cacaggggcc tgtcgcgcag cagccagatc agcagcacgt
     1561 agaggaaggg ctccaccgac gccccgagcg cgctgacgag ttgggcggcc gttcccggcg
     1621 gcgccgcgac gaagaaggcc gaggccatca ccgcgagcca cggccgcaga cccagatcgt
     1681 ggctcagcac gcgcagcagc acgagcgccg tcgtggcgtt gagcagcacg agcggcagct
     1741 tgagggcggc cactgtgggc ccggtcgcca ggaacacggg ggcggccagc caggcctgca
     1801 ccgccagcag gtagttctgc ccgtagtaga agagcgggaa cgcccggcct tcgatcagat
     1861 gcttggccat cagccctgtc acggcctggt ccgagtcgaa cgaggcgtgt tcccacacca
     1921 ggaacgccac gccgcgcagc acggccaccg acaccacgag gactgcggca gccgcgaact
     1981 cccgcgtgga tccgccgcct tccctgcgga caaccgccgg cgcggtgtgc tgaggcatct
     2041 cggaatgacc agtccgaggg tatcacgcgg cagcggcccg ctcgtcgggt gacccgccgg
     2101 cgcggcggcc ggcggccaga gcccgcctcg agcgtgctac cggacgccga agaccccgag
     2161 ggcccgctgc gtggccatcc cgagcatggc ggccgcgggg atcgtgagga gccaggccca
     2221 gacgatccgg ccggccacgc cccagcgcac ggccgagagg cgc
//
LOCUS       DAOSIF010000491         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41244_length_2262_cov_3.883099, whole genome shotgun sequence.
ACCESSION   DAOSIF010000491 DAOSIF010000000
VERSION     DAOSIF010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41244_length_2262_cov_3.883099"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLT35_12935"
     CDS             complement(<1..711)
                     /locus_tag="PLT35_12935"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOG30177.1"
                     /translation="MSGEFERVQAAAAYVKTRISEAPDIAITLGSGLGGFASRVEQAA
                     VVPYGEIPGWPPAGVVGHAGRLVAGRVRGRRVVVLSGRSHFYEGHPMATVVFGTRVVA
                     SLGARTLILTNAAGGINTSFSQGGLMVIDDHLNLMGTNPLVGPNDDRFGPRFPDMSEV
                     YSRRLRIVADRAAERIGLALSHGVYVGLHGPSYETPAEIRYLRSIGGDAVGMSTVAEA
                     IAARHMGIEVLGISCITNM"
     gene            complement(708..2072)
                     /locus_tag="PLT35_12940"
     CDS             complement(708..2072)
                     /locus_tag="PLT35_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HOG30178.1"
                     /translation="MTHTVGTGGRRRARRLSATEWTIIGAGALVALAAVLLASVGGMP
                     KAQPFAGAVVILGIAYLMSADRRAIDLKTVLWGLGLQIVFALIVLKNPWGQQAFRWVG
                     DLITRLLAFANVGAAFVFGPLGDREAWPRIVRGALGDEGAQYASIFAFQVLPTIVFIA
                     ALFAILYYFGVMQVVVRGFAWAMRKVMKASGAETLNVAASIFMGQTEAPLTIRPFLPK
                     MTESELMTVMTSGMAHISGGIMAAYILFGIEARHLLTAVIMTAPGTLMMAKMFVPETG
                     EPETMGTVKLEVERTDVNVIDAAGRGTGEGLHLALNVAAMLISFLSLVALINAILGLA
                     GLSLQQILGWVFSPVAWAMGVPWKDAATIGNLLGTRMVLNEFVAYSQLGPLKASLDPN
                     SFTIATFALCGFANFSSIGIQIGGIGSLAPNRRHDLARLGMRAMLAGTLANFMTATIA
                     GFLL"
BASE COUNT          369 a          827 c          744 g          322 t
ORIGIN      
        1 catgttcgtg atgcacgaga tgcccagcac ctcgattccc atgtgccgcg cggcaatggc
       61 ctcggccacc gtcgacatgc ccaccgcgtc gccgccgatc gatcggaggt aacggatctc
      121 ggccggcgtc tcgtagctgg gcccgtgcag ccccacgtac acgccgtgag agagggccag
      181 gccaatcctc tccgccgccc ggtctgccac gatcctcagc cgcctggagt agacctcgct
      241 catgtccggg aagcgcggcc cgaaccgatc gtcgttcggt ccgaccagcg gattggtgcc
      301 catcaggttc aggtggtcgt cgatgaccat cagcccgccc tgcgagaacg acgtattgat
      361 gccgcccgcc gcgttggtca ggatcagggt tctggcgccc agcgatgcca cgaccctcgt
      421 gccgaacacg accgtcgcca tcggatggcc ttcgtagaag tgcgagcgcc ccgagagcac
      481 caccacccgc cggccgcgga cgcggcccgc caccagcctg ccggcgtgcc cgacgacgcc
      541 cgcgggcggc cagccgggga tctcgccgta cgggacgacg gcggcctgct cgacgcgcga
      601 ggcgaagccc cccaggccgg acccgagggt gatggcgatg tccggcgcct cggatatccg
      661 ggtcttcacg taggccgccg cggcctggac gcgctcgaat tcgccgctca taggaggaac
      721 cccgcgatgg tagccgtcat gaagttcgcc agcgtgcccg caagcatcgc ccgcatgccg
      781 aggcgggcca ggtcgtgccg ccggttgggc gcgagcgatc cgatgccgcc gatctggatg
      841 ccaatcgagc tgaagttcgc gaacccgcac agcgcgaagg tcgcaatcgt gaacgagttg
      901 gggtcgagcg acgccttcag gggcccgagc tgcgagtagg ccacgaactc gttgaggacc
      961 atgcgcgtcc cgagcaggtt gccgatggtg gcggcgtcct tccacggcac gcccatcgcc
     1021 caggccaccg gcgagaacac ccagccgagt atctgctgga ggctcaggcc ggccagcccg
     1081 aggatcgcgt tgatgagcgc cacgagcgac aggaacgaga tcagcatggc cgcgacgttc
     1141 agggcgaggt gcagcccttc gccggtgccc cgtcccgcgg cgtcgatcac gttgacgtcg
     1201 gtccgctcca cctcgagctt caccgtgccc atcgtctccg gctcccccgt ctcgggcacg
     1261 aacatcttgg ccatcatgag cgtgcctggg gccgtcatga tcactgccgt cagcaagtgg
     1321 cgggcctcga tgccgaagag gatgtaggcc gccatgatgc cgcctgagat gtgcgccatg
     1381 ccggacgtca tcaccgtcat cagctccgac tccgtcatct tcggcaggaa gggccggatc
     1441 gtgagcggcg cctcggtctg gcccatgaag atgctggccg cgacgttcag cgtctccgcc
     1501 ccgctcgcct tcatgacctt ccgcatggcc caggcgaagc ctcgcacgac aacctgcatc
     1561 acgccgaagt agtagaggat cgcgaagagc gccgcgatga acacgatcgt cggcaggacc
     1621 tggaacgcga agatcgacgc gtactgcgcg ccctcgtcgc cgagcgcccc gcgcacgatg
     1681 cgcggccagg cctcccggtc gccgagcggg ccgaacacga aggcggcgcc gacgttcgcg
     1741 aaggccagga gccgcgtgat cagatcgccg acccacctga acgcctgctg gccccacggg
     1801 ttcttcagca cgatgagcgc gaagacgatc tgcaggccca ggccccagag caccgtcttg
     1861 aggtcgatgg cccgcctgtc ggccgacatc aggtaggcga tgccgaggat gaccacggcg
     1921 cccgcgaagg gctgcgcctt gggcatgccc ccgacgctgg ccagcagcac cgccgccagc
     1981 gcgaccaggg cgccggcgcc gataatcgtc cattccgtcg ccgacaaccg ccgcgcccgg
     2041 cgcctgcctc ccgtcccgac tgtgtgcgtc atgccgcctc ccgtgggcgc cgcctcagac
     2101 gatgtcgccg acgaccagcg gacggggcgc cgggggcgct ccgccgagtt cgatggcctg
     2161 cctggccaga tccgccgcat cggccagccc gttgccatcc ctgtggatca gttccagcac
     2221 cggatcgccc gcccggagca gggcgccgag cgtcgcaatc ac
//
LOCUS       DAOSIF010000492         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41355_length_2257_cov_3.080381, whole genome shotgun sequence.
ACCESSION   DAOSIF010000492 DAOSIF010000000
VERSION     DAOSIF010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41355_length_2257_cov_3.080381"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..376)
                     /locus_tag="PLT35_12945"
     CDS             complement(131..376)
                     /locus_tag="PLT35_12945"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOG30179.1"
                     /translation="MADPEPHIAQKSPIKVRLEAGKTYYWCACGLSRNQPWCDGSHRG
                     GPFRPLAYVADADGEKWFCACKHTATKPLCDGTHRKL"
     gene            510..>2257
                     /locus_tag="PLT35_12950"
     CDS             510..>2257
                     /locus_tag="PLT35_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOG30180.1"
                     /translation="MKPRACLVIAALLLAAGGPLAQQPQENLSVFDGWMRFSDAPNAL
                     YHHLAGQAFTLLDARRDRVARLRTASEWRHRQEEIRATLDRIVGPFPERTPLNARVTG
                     IVRKDGYRFEKVVFESMPGFFVTGALFVPDGLTARAPAVLYLCGHSASGFRNAPYLTV
                     ILNLVKKGFVVFAMDPVGQGERHQYWDEAQKASRVGGPTLEHSYPGAQCFLTGSSLAR
                     YMIWDGIRAIDYLAGRPEVDASRLGVTGRSGGGTQSAYIGAMDPRIAAAAPENYITTF
                     RRLLESRGVQDAEQNFTSGMASGIDLPDLLVARAPRPTLLIATTRDMFSIQGAREARD
                     EVARAFEAHGAAESFQMVEDDAPHASTVKNREALYAFFRKALNAPGPSADEPVELMPF
                     DELNATPTGQLATSLKGGTVFTINRADAVKRLDALERSRTRPEAHRAAVLASARELSG
                     YRAPGPEAPQAVFTGRWQRDGYAVEMFFIPGEGDYVIPFLVFVPGGGGAHPAVIYLHP
                     EGKAADARPGGVIEGLVKRGYLVLAPDLLGAGEMGPGSFQGDAYAFTLGSANYNYWFA
                     GIQIGRSLTGIRAGDVV"
BASE COUNT          316 a          786 c          821 g          334 t
ORIGIN      
        1 cggcgcccgc cgcgagcgag ccaacgcccc ctcagccgtg cggacaagga gcgtgaaggg
       61 agccgcagtc gtgcggttcg acaaggccgg cgcctcacgc cgcccgcgga acgggcgcga
      121 gcctcccggg ctacagcttc cggtgcgtgc cgtcgcacag cggcttcgtg gccgtgtgct
      181 tgcacgcgca gaaccacttc tccccgtcgg cgtcggcgac gtaggccagc ggcctgaacg
      241 gcccgccgcg gtgcgacccg tcgcaccacg gctggtttct gctgaggccg caggcgcacc
      301 agtaataagt ctttcccgct tcgagccgga ccttgatcgg gctcttctgg gcgatatgag
      361 gctcggggtc ggccatcgtg ggctccttgg cagccgggtt gagtgcggcg cgggcgcctg
      421 gaccgcggaa cggccgcgcg gcgcgccggg agcaattatg gatccgccgg gggccgttcg
      481 ggccacgcag acgccataat gtcggtgcca tgaaaccccg agcctgtctc gtcatcgctg
      541 cgttgctcct cgccgccggc gggcctctcg cgcagcagcc gcaggagaac ctctcggtct
      601 tcgacggctg gatgcggttc tcggacgcgc ccaacgcgct ctaccaccat ctcgccgggc
      661 aggccttcac gctcctggac gcgcggcgcg atcgcgtcgc gcgcctgcgc acggcctccg
      721 agtggcggca ccgccaggag gagatcaggg cgacgctcga ccggatcgtc ggcccgttcc
      781 ccgagaggac gccgctcaac gcgcgagtga cgggcatcgt ccgcaaggac ggctaccgct
      841 tcgagaaggt cgtgttcgag tcgatgccgg gcttcttcgt gaccggcgcg ctgttcgtgc
      901 cggacgggct cacggcccgg gcgccggcgg tcctctacct gtgcgggcac tcggcgtcgg
      961 gctttcggaa cgcgccgtac ctgacggtca tcctgaacct cgtcaagaag gggttcgtcg
     1021 tgttcgcgat ggatcccgtc gggcagggcg agcgccacca gtactgggac gaggcgcaga
     1081 aggcgtcgcg cgtcgggggg cccacgctgg agcactcgta tcccggcgcg cagtgtttcc
     1141 tcaccgggtc gtcgctcgcg cgatacatga tctgggacgg catccgcgcg atcgactacc
     1201 tcgccggccg ccccgaggtg gacgcgtccc ggctcggcgt gaccggcagg tccggcggcg
     1261 ggacgcagtc ggcctacatc ggcgccatgg atcccaggat tgcggcggcg gcccccgaga
     1321 actacatcac gacgttccgg cggctcctcg agagccgcgg cgtgcaggac gccgagcaga
     1381 acttcacgtc cgggatggcc agcggcatcg acctgcccga tctcctggtt gcgagggcgc
     1441 cgcggccgac gctgctgatc gcgacgaccc gcgacatgtt cagcatccag ggggcccggg
     1501 aggcgcgcga cgaagtcgcg cgggccttcg aggcgcacgg ggccgcagag agcttccaga
     1561 tggtcgagga cgatgcgccg cacgcgtcga ccgtgaagaa ccgggaggcg ctgtacgcgt
     1621 tcttccggaa ggcgctgaac gcgcccgggc cgtcggcgga cgagcccgtc gagctgatgc
     1681 ccttcgacga gctgaacgcg acgcccaccg gacagctggc gacgtcgctg aaaggcggga
     1741 ccgtcttcac catcaaccgc gcggacgcgg tgaagcggct cgacgcgctc gaacggtcgc
     1801 ggacgcggcc cgaggcgcac cgcgccgcgg tgctcgcgtc ggcgagggag ctctccggct
     1861 accgcgcgcc gggtcccgag gcgccgcagg cggtcttcac cggccgctgg cagcgcgacg
     1921 gctacgcggt ggagatgttc ttcatccctg gcgagggtga ctacgtcatc ccgttcctgg
     1981 tgttcgtgcc ggggggcggc ggcgcgcatc cggccgtgat ctacctgcat cccgaaggaa
     2041 aggcggccga cgcccggccc gggggcgtca tcgaggggct cgtgaagcga ggctatctgg
     2101 tcctcgcgcc ggacctcctc ggggccggag agatggggcc cggttcgttc cagggcgatg
     2161 cctatgcgtt cacgctcggc agcgccaact acaactactg gttcgccggg attcagatcg
     2221 ggcggagcct gaccggcatc cgcgccggcg atgtcgt
//
LOCUS       DAOSIF010000493         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41384_length_2256_cov_3.746933, whole genome shotgun sequence.
ACCESSION   DAOSIF010000493 DAOSIF010000000
VERSION     DAOSIF010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41384_length_2256_cov_3.746933"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLT35_12955"
     CDS             complement(<1..655)
                     /locus_tag="PLT35_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG30181.1"
                     /translation="MPRPSRVQFFATCLVDVFSPSVGHAAVAVLERLGVGVDVVPNQT
                     CCGQPAFNGGFVEEAAAMARHTIDVLSRSPDPVVVPSGSCADMIVGRYPEIFDGDAVY
                     GPKARALAERTYEFCQFLADVLGAGLPAAASGPALAYHASCHGLRGLGLRAQPRAVLR
                     AVAGGRCRDLPDAEVCCGFGGVFAVKMGPLSGAMLESKLDAVEASGADFVVATDLSCL
                     "
     gene            complement(699..1766)
                     /locus_tag="PLT35_12960"
     CDS             complement(699..1766)
                     /locus_tag="PLT35_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOG30182.1"
                     /translation="MSNPHLFAPLTIRGVTLKNRIGVSPMCQYSATDGFVTDWHVVHY
                     GSRAVGGAGLVLTEAVAVHPRGRISPADLGIWEDQHIPGLRKITEFAKAQGAVPGIQL
                     AHAGRKASTAPPWLGGGPLPQESGGWIPVAPSALPFDAASPVPEALDRPAIEAVIQDF
                     RRAAARARAAGFQAAELHAAHGYLIHQFLSPLSNRRTDEFGGSYDNRVRLVKLVCRAV
                     REEWPADLPLLVRLSATDWVEGGWRPEDTVALAHHLSEYGVDVIDCSSGGNVAGAAMP
                     IGPGYQVPFSQRIKDMLPILTATVGLVTSPAQADQIVRNEQADIVLLARELLRDPYWP
                     LHAARALGQDVSWPNPYLRAR"
     gene            1972..>2256
                     /locus_tag="PLT35_12965"
     CDS             1972..>2256
                     /locus_tag="PLT35_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30183.1"
                     /translation="MSGIGVGARIGAYRLIGEIGNGGLGAVYRAVEEGSGREAAVRVF
                     AREVSGDAVLADRLRTMAPALRRLHHAHIGGVYELVTAGADLALLLEHVPG"
BASE COUNT          327 a          811 c          838 g          280 t
ORIGIN      
        1 tgaggcagct gaggtcggtc gcgacgacga agtccgcgcc gctggcctcg acggcgtcga
       61 gcttcgattc gagcatcgcg cccgacagcg gccccatctt caccgcgaac acgccgccga
      121 aaccgcagca cacctcggcg tccggcagat cgcggcaccg gccgccggcc accgcccgca
      181 gcactgcccg cggctgcgcg cgcagcccca ggccccgcag gccgtggcag gacgcgtggt
      241 acgccagcgc ggggccggag gcggcagcgg gaaggcccgc gccgaggacg tcggccagga
      301 actggcagaa ctcgtaggtc cgctccgcca gcgcccgcgc cttcgggccg tagaccgcgt
      361 cgccgtcgaa gatctccggg tagcgcccca cgatcatgtc cgcgcaggaa cccgacggca
      421 cgaccaccgg atcgggcgag cgcgagagca cgtcgatggt gtgccgggcc atggccgcgg
      481 cctcctcgac gaagcctccg ttgaaggcgg gctggccgca gcaggtctga ttcggcacga
      541 cgtcgacgcc gacgcccagg cgctcgagca ccgcgacggc cgcgtggccg accgacggcg
      601 agaagacatc gaccaggcag gtcgcgaaga actggaccct cgatgggcgc ggcatgtggg
      661 ccctccgctc gcgtgcgcgg acgctacctt accacgggct accgcgcccg caggtacggg
      721 ttcggccacg agacgtcctg gccgagcgcc cgcgccgcgt gcagcggcca gtacgggtcg
      781 cgcagcagct cgcgcgccag cagcacgatg tcggcttgct cgttccggac gatctgatcc
      841 gcctgcgcgg gcgaggtcac caggccgact gtcgccgtca ggatgggcag catgtccttg
      901 atgcgctgcg agaacggcac ctggtagccc gggccgatcg gcatcgcggc cccggcgacg
      961 ttcccgcccg acgagcagtc gatcacgtcg acgccgtact ccgacaggtg atgcgccagc
     1021 gccaccgtgt cctcgggccg ccacccgccc tccacccagt cggtggcgga gagccggacg
     1081 agcagcggca ggtcggccgg ccattcctcg cggacggccc ggcacaccag cttcaccagc
     1141 cgcacgcgat tgtcgtacga ccctccgaat tcgtcagtcc gccggttcga gagaggggag
     1201 aggaactggt ggatcagata gccgtgcgcg gcgtggagtt ccgccgcctg gaacccggcg
     1261 gctctcgccc gcgccgcggc gcggcggaag tcctggatga cggcctcgat ggccggccgg
     1321 tccagggcct ccggcacggg ggaggcggca tcgaacggca acgcgctcgg cgccacgggg
     1381 atccagccgc ccgactcctg cggaagaggg ccgcctccga gccacggcgg ggccgtgctg
     1441 gccttgcgcc cggcgtgggc cagctggatg cccggcaccg ccccctgcgc cttcgcgaac
     1501 tcggtgatct tgcgcaagcc ggggatgtgc tggtcctccc agatcccgag gtccgccggg
     1561 ctgattcggc ctctcggatg cacggccacc gcctcggtca gcaccaggcc ggcgcccccg
     1621 acggcccggc tgccgtaatg gacgacgtgc cagtcggtga caaatccgtc ggtggcactg
     1681 tactggcaca tgggagaaac gccgatacgg ttcttgaggg tcacgccccg gatggtcagg
     1741 ggggcgaaca agtgcgggtt ggacacggtg tcggctcctc ttcaggtgct agcatagctg
     1801 aacccgaagc agcagtggct ccgactccga tcgccgggac cctggccgaa cgctgcggag
     1861 cggtcacggg ggcgatggcc ccagcctgac ggcgccggcc ggcgaagcgg cgggcctcgc
     1921 gggtccgacc gccgcctgcc tggcgcgtgc cgggcctgga ggagcaacgc cgtgagcggc
     1981 atcggcgtcg gcgcgcgcat cggggcctac cggctgatcg gcgagatcgg caacggcggc
     2041 ctgggcgcgg tgtaccgcgc cgtcgaggag gggagcggca gggaggcggc ggtccgcgtc
     2101 ttcgcccggg aggtgtcggg cgacgcggtg ctggcggatc gcctgcggac gatggcgccg
     2161 gcgctgcgga ggctccatca cgcgcacatc gggggcgtct acgagctggt gacggccggc
     2221 gcggacctgg cgctgctgct cgaacacgtg ccgggc
//
LOCUS       DAOSIF010000494         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41495_length_2252_cov_3.266727, whole genome shotgun sequence.
ACCESSION   DAOSIF010000494 DAOSIF010000000
VERSION     DAOSIF010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41495_length_2252_cov_3.266727"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLT35_12970"
     CDS             complement(<1..488)
                     /locus_tag="PLT35_12970"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG30184.1"
                     /translation="MAAYVVTGGAGFIGSHLAEALVARGHRVRVVDSLVTGHRRNLAA
                     VAKAIEFIEGDLAEPDVARAAVSGMDFVLHQAAIPSVPRSVAEPAFCHRANVEATLQV
                     LIASRDAGVRRLVFAGSSSVYGNEPTQPKREEMPADPLTPYALQKLVGEQYARMFTSL
                     YGL"
     gene            complement(530..1041)
                     /gene="rnpB"
                     /locus_tag="PLT35_12975"
     ncRNA           complement(530..1041)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLT35_12975"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1059..>2252)
                     /locus_tag="PLT35_12980"
     CDS             complement(1059..>2252)
                     /locus_tag="PLT35_12980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG30185.1"
                     /translation="GLEALSKLKAAGDLPVVMISGHGTVATAVEATKLGAFDFIEKPL
                     ATERILLAIRNALETTRLRSENVSLRGAVELRHDIVGSSPALREVFEAVRRAAPTGAT
                     VLIRGESGVGKELVARMIHRHSLRARERFVQVNCAAIPDELIESELFGHEKGSFTGAT
                     DKQVGMFELADRGTIFLDEVGDMSLKTQAKVLRVLQEGEVERLGSSRTLKVDVRVIAA
                     TNKELEREIEKGQFREDLFFRLSVIPLSIPPLRDRAEDIPALVRHFADVFTRENNFRP
                     KRFTPAAVQALQRARWKGNVRELRNTIERVIIMTPGDTVDVADLPEPVRPDGGGPASL
                     PEARGERPGTLREFKESAERAFLVEKLRENDWNISRTAETIGTPRSNLYKKLEHYNIR
                     QETDA"
BASE COUNT          312 a          811 c          732 g          397 t
ORIGIN      
        1 agcccgtaga gcgatgtgaa catccgcgcg tactgctcgc cgacgagttt ctggagggcg
       61 tacggcgtca gggggtcggc cggcatctcc tcccgcttcg gctgcgtcgg ctcgttgccg
      121 taaacggacg aagaccccgc gaacacgagc cgcctgacgc ccgcgtcgcg ggaagcaatc
      181 aacacctgga gcgtggcctc cacgttcgcg cggtggcaga acgccggttc tgcgaccgat
      241 cgcgggaccg aggggatcgc cgcctggtgg aggacgaagt ccatgcccga cacggcggcc
      301 cgcgccacgt ccggctccgc gaggtcgcct tcgatgaatt cgatcgcctt cgcgaccgcc
      361 gccaggttcc tgcggtggcc cgtgacgagg ctgtccacta cccgcacccg gtggccccgc
      421 gcgacgagcg cctccgcgag atgcgatccg atgaaccccg cgccgccggt aaccacgtac
      481 gccgccattg acgcccttct cgaacgcacg accctggttc cgaacgctcc gccgcagtcg
      541 agccgtaagc cgaattctgt gtcccctcga ctcggccggg gcagccgcgt taccgcggcc
      601 gtccgaagcc tcgctcgggg cgacgaccat tcctctggtc ccgtcatcac tggcgggatc
      661 gagcgaccaa cccggaggca tggagcgggc cgctctcgtg gcgcctccct atttggtctt
      721 gctccgtgcg gggtttggcc tgccacccgc gttgccgcgg gcgcggtgcg ctcttaccgc
      781 accttttcac ccttacccgc ctccgttcgc cggccccttg cggttccgac gagctccgtc
      841 gggcgaggtc cgaagagcct gccccgacgg cgcgcgaggc acgaaggcgg gcggtatgtt
      901 ttctgtgcca ctttccttca ggtcaccctg accgggcgtt acccggcgca ctgccctgtg
      961 gagttcggac tttcctccct tcccgggcac cggcgccctc gcgggcccgg ccccaaaaac
     1021 agcggtcgtc tggctgactg cggcggaacc ctccccgcct aggcatcggt ctcctggcgg
     1081 atgttgtagt gctccagctt cttgtataga ttactcctcg gcgtgccgat tgtctcggct
     1141 gtcctggaga tattccagtc gttctcccga agcttctcga cgaggaacgc ccgttccgcc
     1201 gactccttga actcgcgcag cgtgcccggc cgctctcccc tggcctccgg gagggatgcg
     1261 ggcccgccgc catccggtct caccggttcg ggcaggtcgg ctacgtccac ggtgtcgccc
     1321 ggcgtcatga tgatgacccg ctcgatggta ttcctgagct cccgcacgtt gcccttccac
     1381 cgcgcccgct gcagggcctg cacggccgcc ggcgtgaagc gcttcggccg gaagttgttc
     1441 tcgcgggtga agacgtcggc gaagtgacgg acgagagcgg gaatgtcctc ggcccggtcg
     1501 cggagcggcg ggatcgacaa ggggatgacg ctcagcctga agaagaggtc ctcgcggaac
     1561 tgccccttct cgatctcgcg ttccagctcc ttgttcgtgg cggcgatgac gcggacatcc
     1621 accttgaggg tccgcgagga cccgagccgc tcgacttcgc cctcctgcag gacgcgcagg
     1681 accttggcct gggtcttcag gctcatgtcg ccgacctcgt ccaggaagat cgtgccgcgg
     1741 tcggcaagct cgaacattcc cacctgcttg tcggtcgcgc ccgtgaagga ccccttctcg
     1801 tgcccgaaga gctcggactc gatcagctcg tcgggaatcg cggcgcagtt cacctgcacg
     1861 aaccgctcgc gggcgcgcag gctgtgccgg tggatcatgc gggcgacgag ttccttgccg
     1921 acgccgctct cgccccgaat gagcacggtg gcgccggtcg gcgcggcgcg gcgcacggcc
     1981 tcgaacacct cgcgcagcgc cgggctcgat ccgacgatgt cgtgccgcag ctcgaccgcg
     2041 ccccgcagcg agacgttctc gctgcgcaga cgcgtggtct cgagggcgtt cctaatcgcg
     2101 agcaggatgc gctcagtcgc caggggcttc tcgatgaagt cgaaggcccc gagtttggtc
     2161 gcctcgacgg ccgtggcgac ggtcccgtgt ccggagatca tcacgacggg caggtcccct
     2221 gcggccttca gcttgctcag ggcctcaaga cc
//
LOCUS       DAOSIF010000495         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41566_length_2250_cov_3.171298, whole genome shotgun sequence.
ACCESSION   DAOSIF010000495 DAOSIF010000000
VERSION     DAOSIF010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41566_length_2250_cov_3.171298"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLT35_12985"
     CDS             <1..199
                     /locus_tag="PLT35_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30186.1"
                     /translation="TAAGRRLRERLDPGLDEVLGELRRLGEAAERARAAAADGFLTLR
                     SFRRGLGPRTAGGSRNEAEGY"
     gene            214..1161
                     /locus_tag="PLT35_12990"
     CDS             214..1161
                     /locus_tag="PLT35_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOG30187.1"
                     /translation="MTVSDEDLFTQETRPATGRLRRLARQGRDGLSLMGRAALRGIIE
                     LAWGSDDLTHAAAIAYYALLSFLPFLLLSMSVLGAVPAGRDLRVEVVEFVLRYFPAQP
                     DFLANNVNVFWGTRIKLGIGGALGLLWASQGVFGAISTAVNHAWAVERPRSFWQHRWF
                     SLLMMAAAGLMLFAALLLLSAAKVRGESWFTLLLARLPFLAGLSGFGSPLSAFLVFVL
                     IVGLIFYFVPNTKVRFRDVWPGAVVTAILWRGAYEAFSWYVRSFAQASVHGSIATVVW
                     FLIWVYVSAVILLFGVEFTAAIPRIRRELRGPALPLNAS"
     gene            1224..2250
                     /locus_tag="PLT35_12995"
                     /pseudo
     CDS             1224..2250
                     /locus_tag="PLT35_12995"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
BASE COUNT          281 a          768 c          818 g          383 t
ORIGIN      
        1 gacggcggcc gggcgtcggt tgcgcgagcg tctcgacccg gggctcgacg aggtcctcgg
       61 cgagttgcgg cggcttggag aggccgcgga gcgggccagg gcggcagcgg cggacgggtt
      121 tctgacgctg cgatcgttcc ggcgcggact gggccccagg accgcgggcg ggagccggaa
      181 cgaggccgag gggtactgaa acgcggatgg cgcgtgacgg tgtctgacga ggaccttttc
      241 actcaggaga cgaggccggc gaccggtcgg ctgcgccggc tcgcccgaca aggccgcgac
      301 ggcctgtcac tgatgggacg ggccgccttg cgcggtatca tcgaactcgc ctggggcagc
      361 gacgacctga cgcacgccgc cgccatcgcc tactacgcgc tcctgtcgtt cctgccgttc
      421 ctgctgctgt cgatgtccgt tctcggcgcc gtcccggcgg gccgcgacct gcgcgtcgag
      481 gtcgtggagt tcgtcctgcg ctacttcccg gcgcagcccg acttcctcgc gaacaacgtg
      541 aacgtgttct ggggaacccg gatcaagctc ggcatcggcg gcgcgctggg cctgctctgg
      601 gcctcgcagg gcgtctttgg cgcgatcagt acggccgtga accatgcctg ggccgtggag
      661 cgcccgcgga gcttctggca gcaccgctgg ttctcgctcc tcatgatggc ggccgccggg
      721 ctgatgctct tcgccgcgct gctcctgctc agtgcggcca aggtaagggg cgaatcgtgg
      781 ttcacgctcc ttctggccag gctcccgttc ctcgcgggcc tgtcgggctt cggaagtccc
      841 ctgagcgcgt tcctggtgtt cgtgctgatt gtcgggctga ttttctactt cgtgccgaac
      901 acgaaggtgc ggttccggga cgtgtggccg ggggccgtgg tgaccgccat tctctggcgg
      961 ggggcctacg aggcgttctc gtggtacgtg cgctcgtttg cgcaggccag cgtccacggc
     1021 tccatcgcca ccgtcgtctg gttcctcatc tgggtgtacg tctcggccgt catcctgctc
     1081 ttcggcgtcg agttcacggc cgcgatcccg cgcatccgcc gcgagctgcg cggacccgcc
     1141 ctgccgctga acgcgtcgta aggaccgtga accgcctcgc gtccgaactc agcccttacc
     1201 tcctgcagca cgcctcgaat cccgtggact ggcatccctg aggcgaggag gcgtttgcgc
     1261 gcgccaggct ggaggatcgc cccgtttttc tttcgatcgg ttacgccacc tgccattggt
     1321 gccacgtcat ggcgcacgaa tccttcgagg atcccgcggt ggcggcgcaa ctgaacgccc
     1381 ggttcgtgcc ggtcaaggtg gaccgggagg aacgccccga cgtggaccgc ctctacatgc
     1441 tgtacgtgca ggcgacgacg ggggccggcg ggtggccgat gagcgtctgg ctcacgcccg
     1501 acctggagcc gttcttcggc ggcacctact tcccgcccga caatcgcggc ggccggcccg
     1561 gattcgcggc gctcctcggc gagattgcgc gggcgtggcg ggacgaccga cgcggggtgg
     1621 ccgacgccgc ccggcgggcg gcggcgcacc tgcggcaact ggccgaatcg cctgcaccct
     1681 ccgccggggg cgtgcccggc acggacgtgc tcgatcgcgc ggtggcggaa ctcgcggcgt
     1741 cgttcgaccg tcagcgcggg ggattcggcc gagcgcccaa gttcccgcgc gcctcggagc
     1801 tgctgttcct gctccaggag taccggcgta cgggatcgga ggcgccgctc gagatggcgg
     1861 tcaggacgtt gcgggcgatc gcgtcgggcg gcatccgcga tcacatcggc ggagggttcc
     1921 atcgctacgc cgtggatccc gactggcgcc tgccgcactt cgagaagatg ctctacgacc
     1981 aggcccagct cgtgattgcg ctgctcgacg cggccgatgc gtcttcggac gaagcgctgg
     2041 ccgagatcgc cggcgagacg ctggactacg tcctgcgcga catgaccggg ccggacgggg
     2101 agttctactc ggccgaggac gccgacagcg tgccgccggc cggcgccatg gcggccgagc
     2161 ggcgggcgtc agaaggcgcg ttctacctgt ggagcgcgga ggaggtcgac acgctcctcg
     2221 gcgaccgcgc gccgctcgtg tccgagcact
//
LOCUS       DAOSIF010000496         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41858_length_2239_cov_3.205586, whole genome shotgun sequence.
ACCESSION   DAOSIF010000496 DAOSIF010000000
VERSION     DAOSIF010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41858_length_2239_cov_3.205586"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLT35_13000"
     CDS             complement(<1..805)
                     /locus_tag="PLT35_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOG30188.1"
                     /translation="MVDTLGDWARTHTCGGLRASDAGRDVLLLGWVHKVRDLGHLVFL
                     DVRDRYGLTQVIADDEALRTRARQLRAEFVIGVKGRVRPRAPEAINRRLPTGEVEVQA
                     AELRVLSEARVPPFVVADDVAASEELRLKYRYLDLRRPSLQANFGLRHRVTMAVRQYF
                     DGAGFWEVETPVLAKSTPEGARDYLVPSRVHPGEFYALPQSPQILKQLLMVSGFDRYF
                     QIVKCFRDEDLRADRQPEFTQIDLELSFPTEEEIFALIEGLFARLLPLVG"
     gene            complement(812..>1154)
                     /locus_tag="PLT35_13005"
     CDS             complement(812..>1154)
                     /locus_tag="PLT35_13005"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOG30189.1"
                     /translation="RLVLAMPEPRRSERRRPLFVAYMGEAARDAALALLRDLRRAELE
                     AHMEYEGRSLKAQMKRADRLRAAFTLILGEEELAAGEVSVKDMATGGQRRVRREAVVE
                     ECRALVNGGRE"
     assembly_gap    1155..1164
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1165..2087)
                     /gene="hisS"
                     /locus_tag="PLT35_13010"
     CDS             complement(<1165..2087)
                     /gene="hisS"
                     /locus_tag="PLT35_13010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOG30190.1"
                     /translation="MIPAIRGTHDILPGEVERWQRAEDISRRLFERYGYAEVRTPIIE
                     REELFAKGTGETTDIVQKEMYAFTDKGGERVTLRPEATPSLVRAFVEHALEQRMAAPK
                     VYLFGPMFRYERPQKGRYRQFHQLDVEVFGMADPAIDAEVVDLAWALVAALGIVGAEL
                     VVNSVGCPACRPAFAAALVAALGADVSRLCRDCQRRAQTNPLRIFDCKVPADQPVIDA
                     LPHSLDFLCDGCRTHFAAVERHLGMYGIPYRVSHRLVRGLDYYTRTTFEILAGGIGAQ
                     NALLGGGRYDGLFRQLGGPDRTGIGFAAGLER"
BASE COUNT          341 a          798 c          783 g          307 t
ORIGIN      
        1 ccccgacgag cggcaggagg cgggcgaaga gcccttcgat cagcgcgaag atctcctcct
       61 cggtcgggaa cgagagctcg aggtcgatct gggtgaactc cggctgccgg tcggcgcgca
      121 ggtcctcgtc ccggaagcac ttgacgatct ggaagtaccg gtcgaagccc gagaccatca
      181 ggagctgctt gaggatctgg ggagactgcg gcagcgcgta gaactcgccc ggatgcacgc
      241 ggctcggcac caggtaatcg cgcgctcctt ccggcgtgga cttggcgagc acgggcgtct
      301 cgacctccca gaagccggcg ccgtcgaagt actggcgcac cgccatggtc acgcggtgcc
      361 gcaggccgaa attcgcttgg agcgacgggc gcctgaggtc cagatagcga tacttcaggc
      421 gcagctcctc ggacgccgcc acgtcgtcgg ccacgacgaa cggcggcacg cgggcctcgc
      481 tgaggacccg cagctcggcg gcctggacct cgacttcgcc cgtcggcagg cggcggttga
      541 tcgcctcggg cgcccgaggc cgcacgcggc ccttcacgcc gatgacgaac tcggcccgca
      601 gctgcctggc ccgcgtgcgg agggcctcgt cgtccgcgat gacctgcgtc aggccatagc
      661 ggtcccgcac gtccaggaag acgaggtgcc cgaggtcgcg aaccttgtgg acccagccga
      721 gcagcaggac gtcgcgcccc gcgtcggagg cccggagtcc gccgcacgtg tgcgtgcggg
      781 cccagtcgcc gagtgtgtcc accacgagtg tctactcccg cccgccattc accaaggccc
      841 ggcactcctc gacgaccgcc tcgcggcgca cgcggcgctg gccgccggtg gccatgtcct
      901 tgacgctcac ctcgccagcg gcgagttcct cttcgccgag gatgagcgtg aaggcggcgc
      961 gcaggcggtc agcgcgcttc atctgcgcct tcaggctgcg gccctcgtat tccatgtggg
     1021 cttcgagctc ggcacgccgc aagtcgcgca gcagcgcaag ggcggcatcg cgggcggcct
     1081 cgcccatgta cgcgacaaac agggggcggc gccgctcgct gcgccgcggc tcgggcatgg
     1141 ccagcacgag ccgcnnnnnn nnnncgctcc aggccggcgg caaagccgat gcccgtacgg
     1201 tccgggccgc ccagctgacg gaacaacccg tcgtaccggc cgccgcccag cagcgcgttc
     1261 tgggcgccaa tgccgccagc caggatctcg aacgtcgttc gcgtgtagta atcgaggccg
     1321 cggaccagcc ggtgcgacac gcggtacggg atgccgtaca tgcccaggtg acgctcgacc
     1381 gccgcaaagt gcgtccggca tccgtcgcac aggaagtcca gcgagtgcgg gagcgcgtcg
     1441 atgacaggct gatccgccgg caccttgcaa tcgaagatgc gcagcgggtt cgtctgcgcg
     1501 cgccgctggc agtcccggca gagccgggag acgtcggcac cgagggcggc gacgagcgcc
     1561 gccgcgaagg cgggacggca ggccgggcac ccgaccgagt tgacgaccag ctccgcaccg
     1621 acgatcccga gggccgccac cagggcccag gccaggtcga cgacctcggc gtcgatcgcc
     1681 gggtcggcca tcccgaagac ctcgacgtcg agctgatgga actggcggta ccgccccttc
     1741 tgcggccgct cgtagcggaa catcggcccg aacaggtaga ccttcggggc cgccatgcgc
     1801 tgttccaggg cgtgctcgac gaacgcccgc acgaggctgg gagtggcctc cgggcgcagc
     1861 gtcacccgct ccccgccctt gtcggtgaag gcgtacatct ccttctggac gatgtctgtc
     1921 gtttcgccgg tgcccttcgc gaagagctcc tcgcgctcga tgatcggggt ccgcacctct
     1981 gcgtagccgt agcgttcgaa cagccgccgc gagatgtcct ccgcgcgctg ccagcgctcg
     2041 acctcgccgg gcaggatgtc gtgggttcct cggatcgctg ggatcatggc gtgagcaggt
     2101 cctcgccggg ccgcaccgcg cgcccccgca ggcggcgctc gcgcgtcatc gcctgaagac
     2161 ggccatcaca atcgtcagca ccaggctgac gaggacggcg gtggtgatcg gcaggcagaa
     2221 ggtgacggcg ccccggcgg
//
LOCUS       DAOSIF010000497         2236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_41925_length_2236_cov_4.804677, whole genome shotgun sequence.
ACCESSION   DAOSIF010000497 DAOSIF010000000
VERSION     DAOSIF010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41925_length_2236_cov_4.804677"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="PLT35_13015"
     CDS             complement(<1..1569)
                     /locus_tag="PLT35_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOG30191.1"
                     /translation="MPTSERRVTALACGALLVAGAALVASFGQDLPRRTLGGGTPGTK
                     QGLGSRDCLGCHKDFAAKYVGLPHVHAGVKDGGCETCHLRHGLVAKRVMKKDGNDLCY
                     TCHSQDALGLNKAHVHAAAKSGSCVGCHNPHASRHAHLLSAEPTRVCYQCHDRAAYEQ
                     RSVHAVLQAEGCSACHSAHGSDQPGLLVRDERTLCLGCHQAAEPSFAKAHGGYPVERA
                     SCSTCHSPHSSAQPKLLKTSVHAPVAEVQCETCHSAPDAAQPFGVNATGGALCAGCHD
                     AAAMKGTGPVQHVPFKDGECLLCHAPHASASASLLKANGSALCFTCHADVTARPAFAH
                     APVDSDRACLSCHGAHSAEHAQLLTAEPGALCLTCHASTVAAIEKLPAQHPPAAAGEC
                     TACHDAHGSQVAGILKDRMDRVCYACHSDAESKFLKPYTHTPVREGACSSCHRPHGSD
                     QAGLLRAAGAALCASCHGAFMKPLESGSRHEPFVDGQCLSCHDSHSGNRRGMTLDQTG
                     TLCAACHSDVTLQLD"
     gene            complement(1569..>2236)
                     /locus_tag="PLT35_13020"
     CDS             complement(1569..>2236)
                     /locus_tag="PLT35_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG30192.1"
                     /translation="NERKEEALQATIGRRLLNMEALRLGIDKTNAYRDRVRGYQDTLV
                     FERFVQRAIAPENKMREEEVRRYYDGHIKDYSSPGMLRLRGIAFARRTAAEDAMRKLQ
                     QGADFGWLAANAAGQADKGAAALLSFDGRPVTISSMPAGLQKALAGAKTGESRLYASP
                     EGHYYVVSVRQAIDSQPRPYADVREEIARKLYDEKITKGVEEYAARLRAHSKVEIYLK
                     RMR"
BASE COUNT          289 a          801 c          782 g          364 t
ORIGIN      
        1 atccagctgc agcgtcacgt ccgagtggca ggcggcgcac agcgttcccg tctggtccag
       61 cgtcatgccc cgccggttgc cggaatgaga gtcatggcag gagaggcact ggccgtccac
      121 gaagggttcg tgccgcgagc cgctctccag aggcttcatg aaggccccgt gacacgacgc
      181 gcagagggca gcgcctgcgg ccctgagcag ccccgcctgg tccgacccgt gcggccggtg
      241 gcacgacgag cacgcgccct ccctgaccgg cgtgtgcgtg tacggcttga ggaacttcga
      301 ctcggcgtcc gagtgacagg catagcagac gcggtccatc cggtccttca agatgccggc
      361 cacctgcgag ccgtgcgcgt cgtggcacgc cgtgcactcg ccggcggccg ccggcgggtg
      421 ctgggccggg agcttctcga tcgccgcgac ggtcgaggcg tggcacgtca agcacagcgc
      481 gcccggctcc gccgtcagca gctgcgcgtg ctccgccgag tgcgccccat ggcacgacag
      541 acacgcccgg tcgctgtcca ccggggcgtg cgcgaacgcg gggcgggctg tgacgtccgc
      601 atggcaggtg aagcagagcg cgctcccatt cgccttgagc agcgacgcgc tcgcggaagc
      661 gtgcggcgcg tggcacagca ggcactcgcc gtccttgaag gggacgtgct gcaccggccc
      721 ggtccccttc atggcggcgg catcgtggca cccggcgcac agcgcgccgc ccgtcgcgtt
      781 cacgccgaag ggctgcgcgg catcgggcgc ggaatggcac gtctcgcact ggacctcggc
      841 gacgggcgca tggacgctgg tcttcaggag cttgggctgg gcagagctgt gcgggctgtg
      901 gcaggtggag caggaggccc gctcgaccgg gtaccctccg tgggccttgg cgaacgatgg
      961 ctcggccgcc tggtggcacc cgaggcacag ggtccgctcg tcgcggacga ggaggcccgg
     1021 ctggtccgag ccgtgcgcgg aatgacaggc cgagcacccc tcggcctgca gcaccgcgtg
     1081 cacgctccgc tgctcgtacg ccgccctgtc gtggcactgg tagcagaccc gggtcggctc
     1141 tgccgacagg aggtgggcgt gccgcgatgc gtgcgggttg tggcagccca cgcacgaccc
     1201 gctcttcgcg gccgcgtgaa cgtgggcctt gttcagcccg agcgcgtcct gggaatggca
     1261 cgtgtagcag aggtcgttgc cgtccttctt catcacgcgc ttcgcgacca ggccgtggcg
     1321 gaggtggcac gtctcgcagc cgccgtcttt cacgccggcg tggacgtggg gcaggcccac
     1381 gtacttggcc gcgaaatcct tgtggcagcc caggcagtcc cgcgagccga ggccctgctt
     1441 cgtgccaggc gttcctccgc cgagcgtccg cctcgggagg tcctggccga aggaggcgac
     1501 gagcgccgcg cccgcgacga gcagggcgcc gcacgcgagc gctgtgaccc gcctttcgct
     1561 tgtcggcatc accgcatcct cttcaggtag atctcgacct tcgagtgcgc acgcagcctg
     1621 gcggcgtact cctccacgcc cttcgtgatc ttctcgtcgt acagcttccg cgcgatctcc
     1681 tccctgacgt ccgcgtacgg cctgggctgc gagtcgatgg cctgccggac cgagacgacg
     1741 tagtagtgtc cctccgggct cgcgtacagc cgcgactcgc cggtcttggc tcccgccagg
     1801 gccttctgca ggcccgcggg catgctgctg attgtcacgg gccgcccgtc gaagctcagc
     1861 agcgccgcag cccccttgtc cgcctggccc gcggcattcg cggcgagcca gccgaagtcc
     1921 gcgccctgct gcagcttccg catcgcgtcc tcggccgccg tccgccgcgc gaaggcgatg
     1981 ccgcgcagcc tcagcatgcc cgggctcgaa tagtccttga tgtgcccgtc gtagtagcgc
     2041 ctgacttctt cttcccgcat cttgttctcg ggagcgatgg cccgctggac gaagcgctcg
     2101 aagacgaggg tgtcctggta gcccctgacc cggtcgcggt aggcgttcgt cttgtcgatg
     2161 ccgagcctga gggcctccat gttcagcagc ctccggccga tggtcgcctg cagggcttcc
     2221 tccttccgct cgttca
//
LOCUS       DAOSIF010000498         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42027_length_2232_cov_4.759302, whole genome shotgun sequence.
ACCESSION   DAOSIF010000498 DAOSIF010000000
VERSION     DAOSIF010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42027_length_2232_cov_4.759302"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..2036
                     /locus_tag="PLT35_13025"
     CDS             84..2036
                     /locus_tag="PLT35_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG30193.1"
                     /translation="MRRLPTLVAAAGLVCLVSSSVPAGAQPSAAPSAPPPAAPSADAT
                     LTRAGVGAIRGLVLDTQGRPLGGAMVSALGSTVAFALTDRDGRFLLSALPPGAYVVRV
                     HLDGFAPSPRQMVEVGPAAAPSIVSVAMKTLARPPDTPDGRRVLAAGLVSFAAESPAA
                     PPEPSGADEGEADRSETAWRLRHLKRGVLKTIDIDVAAPARAEAPADDPAREPFTGRL
                     FVPSARYAASLFNDFPLTGQVNLVTTSSFGGPAFDPRTPSNSIESSTVAYLSIGSRAG
                     ALGSWSVSAATSPGNVGSWFVDGSLVGRTLGSHRYVAGLAYSSQRITTAEPFAFNAIR
                     SVSRSIGRVYAFDEWAVSKRVGFGYGLSYLWQDYLPSQRLLSPRVAVTVSPADRFRVK
                     AVLSRSALAPGSDEFLHALNSPAGLWLPAQRAFSPWSPRDGLHAQTTDHFELGVEHDI
                     AAYVVGVRAFRQSVRNQAGALFAAPSVAQPSASQSHYYLATLGDVTADGWGIRVSRPF
                     LGAVRGSVEYSEMRPEWIGGAANAIAQWFGAGGQPDGAAVRDLTASLETDVAPTATRI
                     FVLYKLNSGFARQAPDSQKAGPDRRFDVQVNQSLPFLRFTQADWEVLLAVCNLFSDAA
                     GERSMYDELLVIRPPTRVVGGLRVRF"
BASE COUNT          265 a          814 c          798 g          355 t
ORIGIN      
        1 caccgtcacg gcctgacgcc gaggggccgg agcctgccgg cggacgcgtc ggcgaggccg
       61 gtgacgaact ggcgagagat gggatgagac gcctccccac gctcgtggct gcggcgggcc
      121 tcgtgtgcct cgtctcgtcg tccgtgccgg cgggcgccca gccgtctgcc gccccgtctg
      181 cccccccgcc tgccgccccg tctgccgacg ccacgctcac ccgcgccggc gtcggagcca
      241 tccggggcct ggtcctcgac acccagggcc ggccgctcgg gggcgccatg gtgtcggcgc
      301 tcggctccac ggtcgcgttc gccctgaccg accgcgacgg caggttcctg ctgagcgcgc
      361 tgccgcccgg ggcctacgtc gtgcgggtgc atctcgacgg tttcgccccg tcgccgcgcc
      421 agatggtgga ggtggggccc gccgccgcgc cgtcgatcgt gtcggtggcc atgaagacgc
      481 tcgccaggcc gcccgacacg ccggacgggc gccgggtcct ggcagcgggc ctggtttcgt
      541 tcgccgcgga gtcgcccgcc gcgccgcccg aaccctcggg ggcggacgag ggggaggccg
      601 atcgcagcga gaccgcctgg cggctccgcc acctgaagcg cggtgtcctg aagaccatcg
      661 acatcgacgt cgcggcgccg gcccgggcag aggctccggc cgacgatccg gcgcgcgagc
      721 cgttcaccgg ccggctgttc gtgccgtcgg cacgatacgc ggcgtcgctg ttcaacgact
      781 tccccctgac cgggcaggtg aacctcgtca ccaccagctc gttcggcggc ccggccttcg
      841 atccgcgaac gccttcgaat tcgatcgagt cgtcgacggt ggcgtacctc tcgattgggt
      901 ctcgcgccgg cgccctcggt tcgtggtcgg tgagcgccgc gacgagcccc ggcaatgtcg
      961 ggtcctggtt cgtggacggc tccctggtcg gccggacgct cggcagccat cgctacgtgg
     1021 ccggcctggc ctacagcagc cagcggatca ccacggccga gccgttcgcc ttcaacgcga
     1081 tccggagcgt ctcgcggtcg attggccgcg tctacgcgtt cgacgagtgg gccgtctcga
     1141 agcgggtcgg gttcgggtac gggctgtcct acttgtggca ggactacctc ccgtcgcagc
     1201 ggctgttgag cccgcgggtg gccgtgacgg tcagtcccgc cgatcggttc cgggtcaagg
     1261 ccgtgctctc ccggtccgcc ttggcgccgg gctcggacga gttcctccac gcgctgaact
     1321 cgccggccgg tctgtggctg ccggcgcagc gcgcgttctc gccctggtcg ccccgcgacg
     1381 gcctccacgc gcagacgacg gaccatttcg agctcggcgt cgagcacgac atcgcggcct
     1441 acgtcgtcgg ggtgagggcg ttccggcaga gcgtgcggaa ccaggccggc gcgctgttcg
     1501 ccgctccctc cgtggcccag ccgtctgcca gccagagcca ctactacctt gccacgctcg
     1561 gcgacgtgac cgccgacggc tggggcatca gggtgagccg gccgttcctg ggggccgtgc
     1621 gcgggtcggt cgagtactcg gagatgcgtc ccgagtggat cggcggggcc gcgaacgcga
     1681 tcgcccagtg gttcggcgcg ggcggccagc cggacggcgc ggccgtccgc gatttgacgg
     1741 cgtcgcttga aacggacgtg gcgccgaccg ccacgcggat tttcgtgctg tacaagctga
     1801 actccgggtt cgcccgccag gcgcccgact cccagaaggc gggccccgac cggcgcttcg
     1861 acgtgcaggt caaccagtcg ctgccgttcc tccgcttcac gcaggccgac tgggaagtcc
     1921 tgctggccgt ctgcaacctg ttcagcgacg cagccggcga gcgctccatg tacgacgagc
     1981 ttctcgtgat ccgcccgccc acccgagtgg taggaggcct ccgggtccgt ttctagcccc
     2041 gttcagccca ggccggcccg cgagcgatcc actcggttga aggggtcatt cgcggcgttg
     2101 gattccgtct cggttcggcg gcgaagaaga tgcgcaaatg gctggtttca caggactcgc
     2161 atgacatgcc gggcggtgcg ccgctggtat gtcgattgct gcgcctgccg taggcaggac
     2221 tgcgcccgaa cg
//
LOCUS       DAOSIF010000499         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42056_length_2231_cov_3.927390, whole genome shotgun sequence.
ACCESSION   DAOSIF010000499 DAOSIF010000000
VERSION     DAOSIF010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42056_length_2231_cov_3.927390"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1370)
                     /gene="rsgA"
                     /locus_tag="PLT35_13030"
     CDS             complement(297..1370)
                     /gene="rsgA"
                     /locus_tag="PLT35_13030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOG30194.1"
                     /translation="MTLEALGWDGFFAGHFAEIGGKGLVPARVTLEHQHIYSVRTASA
                     ELLASVAGGMRHRASGPRDFPAVGDWVALRPGGHGHRAVIRAVLPRRSRFSRKVAGET
                     TVEQVVAANVDTVFIVMGLDGDFSVRRVERYLVTAWDGGVAPVVVLNKADVCEDVEGA
                     VREVEAVAPGVPVHAMSTKFDRDLAPIAPYLRSASTIALLGSSGVGKSTIVNRLAGAD
                     VQRTADVRASDQKGRHTTSRRQLIELPGGALLIDTPGMRELQLWEGASGITAAFEDIE
                     ALAAGCRFGDCRHDTEPGCAVKAAAGEGRLAAGRLASYRVLQREREALAARQDERGLI
                     EKKRQDKILGRAIKAYHSRSRTR"
     gene            1583..2200
                     /locus_tag="PLT35_13035"
     CDS             1583..2200
                     /locus_tag="PLT35_13035"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOG30195.1"
                     /translation="MKRLVLVALLMGGAGAGLAQAQTWTIDPSHSAASFAVRHMMVST
                     VRGDMGKVTGTAVYNPQQPTAASVEAAIDVAGINTREPSRDSHLRSPDFFDVARFPTI
                     VFTSKKIEAAANGHYRITGNLTMKGVTKEVVLDAEPMRPIIKDQQGVSRTGTTATTKI
                     NRQDFGLTWSRTLDGGGLVVGDEVSVTIDVELISPPPAGRCPDSC"
BASE COUNT          324 a          823 c          774 g          310 t
ORIGIN      
        1 cctcgatccg cttcgcctca cgctcgtcga cgggcacggc cgccgcggcg gcggcgaccc
       61 acaaggcgac gctgacgtgg gcgagcgaac ccatgcggcg cttcctccct ccgacgacgg
      121 cgcgggcgga gcaagccgcg gcgccgcgcc gtcggttcca ggcggcggcg gtgccccgac
      181 gcatccggcc gtatcggcct gcacccgccc gggcgaaggt tcagtttact gcaactgcgg
      241 cgcgcactcc agcgcggatg gcgcgcccgg gccgccgtcg cccgcccgcc cgccgctcac
      301 cgcgtcctgc tgcgcgagtg gtacgccttg atcgcccgcc cgaggatctt gtcctggcgc
      361 ttcttctcga tcaggccccg ctcgtcctgg cgggcggcca gcgcctcgcg ctcccgctgg
      421 agcacccgat agctcgccag ccggccggcg gcgaggcggc cctcgcctgc agcggccttc
      481 acggcgcagc ccggctcggt gtcgtggcgg cagtcgccaa accggcatcc agccgccagg
      541 gcttcgatgt cctcgaaggc cgccgtgatg cccgaggcgc cctcccacag ctggagctcg
      601 cgcatacccg gcgtgtcgat caggagggcg ccgccgggga gctcgatcag ctggcgccgc
      661 gacgtcgtgt gccgcccctt ctgatcgctc gcgcgcacat cggccgtgcg ctggacgtcg
      721 gcgcctgcga gccggttcac gatggtggac ttgccgacgc cggacgaccc gagtagcgca
      781 atcgtgctgg cggaacgcag atagggcgcg atgggggcga ggtcgcgatc gaacttcgtg
      841 ctcatcgcat gcaccggcac gcccggcgcc acggcttcca cctcgcggac ggctccctcc
      901 acgtcctcgc acacgtcggc cttgttcagg acgaccaccg gcgctacgcc gccgtcccag
      961 gccgtgacga ggtaccgctc gacacggcgg acgctgaaat cgccgtccag ccccatcacg
     1021 atgaacaccg tgtccacgtt ggcggcgacc acctgctcga cggtcgtctc gccggcgacc
     1081 ttgcgcgaga agcggctcct ccgcggcagg acggcccgga tgacggcgcg gtgaccgtgg
     1141 ccgcccggcc gcagcgcaac ccagtcgccc actgccggga agtcgcgcgg gccggacgct
     1201 cgatggcgca tgccgccggc gacgctggcg agcagctcgg cggacgcggt gcgaacgctg
     1261 tagatatgct ggtgttcgag cgtcacccgg gccggcacca ggcccttgcc gccgatctcg
     1321 gcgaagtggc ccgcgaagaa gccgtcccag cccagcgcct cgagtgtcat gggaggagcc
     1381 tagcacacgc ctgagcggga tggcggcgcg gcgacgcgcg tggcgcaggc ctgcgccgaa
     1441 tagcgaggcg cccgggttcg cctcggcgcc cgacctcgga tcgggtcgaa accaaccggc
     1501 ggcggcacga atcctatgat acggcgcggt gtgcgaatgc ccgctcgatg ctcggcagcc
     1561 ccgcggcctt ccggaggtgt gcatgaagcg ccttgttctc gttgcgctct tgatgggcgg
     1621 cgccggcgcc ggccttgccc aggcgcagac ctggacgatc gacccgtccc actcggcggc
     1681 gtccttcgcc gtgcgccaca tgatggtctc caccgttcgc ggcgacatgg gcaaggtgac
     1741 cggcacggcg gtctacaacc cgcagcagcc cacggccgcg tcggtcgagg ccgccatcga
     1801 cgtcgccggc atcaacaccc gtgaacccag ccgcgactcg cacctgagaa gcccggactt
     1861 cttcgacgtc gccagattcc cgacgatcgt cttcacgtcg aagaagatcg aggccgcggc
     1921 gaacggccac taccggatca cgggcaacct gacgatgaag ggcgtgacga aggaggttgt
     1981 gctggacgcc gaacccatgc ggccgatcat caaggaccag cagggcgtca gccgcaccgg
     2041 cacgaccgcc acgacgaaga tcaaccggca ggacttcggc ctcacgtgga gccgcacgct
     2101 cgacggcggc ggtctggtgg tgggcgacga ggtgtcggtg acgatcgacg tcgagttgat
     2161 ctcgccgccg ccggcggggc gatgtcccga tagctgttga aaattggagc ggcggctccg
     2221 tcgctgctcg t
//
LOCUS       DAOSIF010000500         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42062_length_2231_cov_3.181066, whole genome shotgun sequence.
ACCESSION   DAOSIF010000500 DAOSIF010000000
VERSION     DAOSIF010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42062_length_2231_cov_3.181066"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..591
                     /locus_tag="PLT35_13040"
     CDS             58..591
                     /locus_tag="PLT35_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30196.1"
                     /translation="MGSSTGPMTPQQVWREMSAEQRLAAARALWADEDSAPQQAEAIQ
                     ALARQLRFRPQSLLRLPPDRLARHLAGVRAISAPLASRALVVYHLAGQRPMLEAFLAH
                     LGVPHERGVISDGPTEPPGEDRLQAAAAALLERFPAADVRVYLLTLAAQDPGVWGRLA
                     PIADAIAAGTPGAADAP"
     gene            674..1252
                     /gene="aat"
                     /locus_tag="PLT35_13045"
     CDS             674..1252
                     /gene="aat"
                     /locus_tag="PLT35_13045"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOG30197.1"
                     /translation="MLAPSLLVAAYEAGYFPMAMDDGAIRWFSPDPRAVIPLARFRPS
                     RRLLRTMRSGRYAVTVNRAFRAVMEACASGRDEGTWISPEILESYCALHARGLAHSVE
                     AWSGANLAGGLYGVALNGAFFGESMFHRASDASKVALASLVARLRERGFRLLDVQWQT
                     PFMAACGAVEVPRDEYLADLQEALRAEARFDG"
     gene            1289..2062
                     /gene="udp"
                     /locus_tag="PLT35_13050"
     CDS             1289..2062
                     /gene="udp"
                     /locus_tag="PLT35_13050"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOG30198.1"
                     /translation="MAVRTVYHLDLDSAKTEGAALALLPGDPGRVEALARTAPFERGR
                     ELACKREYCTWIAYLGRTPVLVTSTGIGGPSASIAIDELAQLGVHTLLRVGTTGAIQA
                     DIDVGTVIVTTGAVRLDGASRHYAPIEYPAVAHPEVVQAAISAASALGIACRAGVSAS
                     CDTFYPGQERYDSYTGYVPRRFQGATEEWRRLHVLNYEMESSTVLTLTAAMGLRGGCV
                     AGVVVNRTRSEAITRADLEAGETHAVRVAVGAMARLAGR"
BASE COUNT          267 a          800 c          859 g          305 t
ORIGIN      
        1 tgctaagctc aacgcagtac cgggcggccg cggcggcggc tgggaggagc cgagggcatg
       61 gggtcgagta cggggccgat gactccgcag caggtgtggc gggagatgag cgccgagcag
      121 cgcctggccg ccgcccgtgc gctctgggcg gacgaggatt ccgcgcccca gcaggcagag
      181 gccatccagg cgctcgcgcg ccagctccgg ttcaggcccc agagcctgct caggctgccc
      241 cctgaccgac tcgcacggca cctcgccggc gtgcgcgcga tctcggcgcc gctcgcgagc
      301 cgcgcgctcg tggtgtacca tctcgccggg cagcggccga tgctggaggc gtttctcgcg
      361 caccttgggg tgccgcacga gcgcggggtc atctcggacg gcccgaccga gccgcctggc
      421 gaggaccggc tgcaagcagc ggccgcggcg ctcctcgagc gcttcccggc ggcggacgtc
      481 cgcgtctacc tgttgacgct ggcggcgcag gacccgggcg tctggggccg gctcgcgccg
      541 attgccgacg cgatcgcggc agggacgccg ggggcggctg acgcgccctg atctccggcc
      601 gcgcgccggc cgcgcccgtc tgccgcggcc gcgcgtcttg acgcgccggc gcggctgtca
      661 ggaagcacgt gccatgcttg caccgtcgct cctcgtcgcc gcctacgagg cgggctactt
      721 cccgatggcg atggacgacg gcgccatccg gtggttctcg cccgacccgc gcgccgtcat
      781 cccgctggcg cgcttccggc cgtcacgccg gctgctgcgg acgatgagga gcgggcggta
      841 cgcggtgacg gtgaaccgcg cgtttcgcgc cgtgatggag gcctgcgcgt ccggccggga
      901 cgagggcacg tggatcagcc ccgagatcct cgagagctac tgcgcgctgc acgcgcgcgg
      961 gctcgcgcac tcggtggagg cctggtccgg agcgaatctg gccggaggcc tgtacggggt
     1021 ggcgctgaac ggcgcgttct tcggcgagtc gatgttccac cgcgcgagcg acgcgtcgaa
     1081 ggtcgccctg gcgtcgctgg tcgcgcggct gcgggagcgg gggttccggc tgctcgacgt
     1141 ccagtggcag accccgttca tggccgcgtg cggcgccgtc gaggtcccgc gcgacgagta
     1201 cctggcggac ctgcaggagg cgctgcgggc cgaggcccgg ttcgacggct gacggcggtc
     1261 cggccgcgcg ccggggggag gaccgatcat ggcggtgcgg acggtgtatc acctggacct
     1321 cgactcggcg aagaccgagg gcgccgcgct cgcgctgctg ccgggagatc ccggccgcgt
     1381 ggaggcgttg gcgcgcaccg ctcccttcga gcgaggccgg gagctggcgt gcaagcggga
     1441 gtactgcacg tggatcgcgt acctgggccg gacgcccgtg ctcgtcacct caacgggcat
     1501 cggcggcccg tcggcgtcga tcgccatcga cgagctggcc caactcggcg tccacacgct
     1561 cctgcgggtg ggcacgacgg gcgcgatcca ggccgacatc gacgtcggca ccgtcatcgt
     1621 gacgacgggc gccgtgcgcc tcgacggggc ctcgcgccac tacgcgccca tcgagtaccc
     1681 cgccgtcgcc cacccggagg tcgtgcaggc ggccatctcg gccgcctcgg cgctgggcat
     1741 cgcgtgccgc gccggcgtca gcgcgtcgtg cgacacgttc tatcccggcc aggagcgcta
     1801 cgattcctac acggggtacg tgccgaggcg cttccagggc gccaccgaag agtggcggcg
     1861 gctccacgtg ctgaactacg aaatggaatc gtcaaccgtc ctcacgctga ccgccgccat
     1921 gggcctccgc ggcggatgcg tcgccggcgt ggtcgtcaac cgcacgcgca gcgaggccat
     1981 caccagggcc gacctcgagg ccggtgagac ccacgccgtg cgcgtggcgg tcggggcgat
     2041 ggcgcgtttg gcggggcgct gagccgctgg gccggcctgc gcgccgcctg cgccgggaaa
     2101 ggggcaggga tgagcgccgt tgacgtggtg ttcctcggga ccggcgacgc cttcggcagc
     2161 ggcggacgcc tgcacagctc ctcgctcctg cggtcggccg acgggagcgt tctcgtcgac
     2221 tgcggcgcct c
//
LOCUS       DAOSIF010000501         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42134_length_2228_cov_2.956742, whole genome shotgun sequence.
ACCESSION   DAOSIF010000501 DAOSIF010000000
VERSION     DAOSIF010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42134_length_2228_cov_2.956742"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLT35_13055"
     CDS             <1..468
                     /locus_tag="PLT35_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30199.1"
                     /translation="NMLLTRDHPPFRFHDPKRPIFTRPRFLTASRFIDSCVSHSMVAD
                     GCYINRCRIETSIVGIRTSIGEGALVRRSVLLGADFYGTDEEETGDPSVPLGIGADSV
                     LDRVIVDKNARIGRGVRLVNERGLVDADGDNYFIRGGIIIVPKGAVVPDGTVV"
     gene            complement(490..996)
                     /gene="sixA"
                     /locus_tag="PLT35_13060"
     CDS             complement(490..996)
                     /gene="sixA"
                     /locus_tag="PLT35_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HOG30200.1"
                     /translation="MTDCSLYLVRHGAAAEHGPSYPNDDLRPLTGEGIERLRLEAAGL
                     AALDVRLDRILTSPLVRAVQTAEVLAAGVRCAAPLVTAEALRPGGRFEALVAELAHTG
                     SARSVALVGHLPSIGQFAAALIGATRALSFDTGGVCRIDVASLPPAAGAGRLVWHAPP
                     RALRALGR"
     gene            1256..2185
                     /locus_tag="PLT35_13065"
     CDS             1256..2185
                     /locus_tag="PLT35_13065"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG30201.1"
                     /translation="MRTRSLTAAVRLSLLASALVVSSVCAAPALAPDPPAFDGGRAFE
                     HLRAIVGIGPRTPGSPAAAETRRYIEAQMAAIGVPVAAQAFEAKTPIGPVKMVNLRAT
                     VAGASADRIVIGGHYDTKLFKTFRFVGANDAGSSTAFLIEMARVLKARRNPLTIELVF
                     FDGEEAFVDWYLGDDHTYGSRHYVQEASRIGDLARIKAMILVDMIGDRDLLIKRESNS
                     TAWLTDIIWSSAKALGFGAHFAPDAISVEDDHIAFLQAGVPAVNLIDMDFPAWHTPDD
                     TLDKVSARSMEVVGRVLAAALPKIEARLLGRAR"
BASE COUNT          332 a          829 c          765 g          302 t
ORIGIN      
        1 aacatgctcc tcacgcggga ccacccgccc ttccggttcc acgacccgaa gcggccgatc
       61 ttcacgcggc cccgcttcct caccgcctcg cgcttcatcg acagctgcgt cagccactcg
      121 atggtcgccg acggctgcta catcaaccgc tgccggatcg agacgtcgat tgtcgggatc
      181 cggaccagca tcggcgaagg cgcgctcgtc cgccggtccg tgctgctggg cgccgatttc
      241 tacggcaccg acgaggagga gaccggtgac ccgtcggtgc ccctcggcat cggcgcggac
      301 tccgtgctgg atcgggtcat cgtggacaag aacgcccgga tcggccgcgg cgtgcggctg
      361 gtgaacgagc gggggctcgt cgacgccgac ggcgacaact acttcatccg cggcggcatc
      421 atcatcgtgc ccaagggcgc cgtggtgccc gacggcaccg tcgtctaggc ggagagcccg
      481 gcgccggcct cagcgcccaa gcgcgcgcag cgcccgcggc ggggcgtgcc agaccagccg
      541 ccccgcgccg gccgctggcg gcagcgaggc cacgtcaatc cggcagacgc cgcctgtgtc
      601 gaaggacagc gctcgggtcg cgccgatgag cgccgcagcg aactgcccga tcgacggcag
      661 gtggccgacc agcgccaccg agcgggcgct gcccgtgtgc gccagctccg cgacgagcgc
      721 ctcgaagcgg ccgccgggcc tgagcgcctc ggctgtcaca agcggcgccg cgcaccggac
      781 gccggccgcc aggacttcgg cggtctgcac ggcccgcacg agggggctcg tcaggatgcg
      841 gtcgaggcgg acgtcgagtg ccgccagccc ggcggcctcg agccgaagcc gctcgatgcc
      901 ttccccggtc aggggccgga gatcgtcgtt gggatacgac ggcccgtgtt ccgccgccgc
      961 gccgtggcgc accaggtaca acgagcaatc ggtcatgtca ctcctccagc gggcgtcgcg
     1021 gccgcgcgcg gcgcgcccgg gcccgcttgc gctcgacgct ggcctgcagc aatctcgcgg
     1081 ccgcggcggt ccccccacgg cgtccctggc gagcaactgc atcatgacat cgatcttccg
     1141 gctcggaccg acgaggcgcg tgagggccga gccgcgggga ttgccgcgca agcccgcgca
     1201 accgccagcc gagcggaagg aggactgcgc gtcggaggta tactgaagac acgctatgcg
     1261 tacgcgctcc ctcaccgccg ctgtacggct ctcgctgctt gcgtccgcgc tggtcgtctc
     1321 ctccgtgtgc gcggcgccgg cgctcgcgcc ggacccgcct gccttcgacg ggggcagggc
     1381 gttcgagcac ctccgcgcca tcgtcggcat cggccccagg acgccgggct cgcccgcggc
     1441 ggccgagacc aggcgctaca tcgaggccca gatggccgcc atcggcgtgc cggtcgcggc
     1501 gcaggcgttc gaagcgaaga cgcccatcgg gccggtgaaa atggtgaacc tccgcgccac
     1561 cgtcgccggc gcgagcgccg accgcatcgt catcggcggc cactacgaca ccaagctgtt
     1621 caagacgttc cggttcgtcg gcgcgaacga cgccggatcg agcacggcat tcctcatcga
     1681 gatggcccgc gtgctgaagg cccgcaggaa cccgctcacc atcgaactcg tgttcttcga
     1741 cggagaggag gccttcgtcg actggtacct cggcgacgac cacacctacg gcagccgcca
     1801 ctacgtgcag gaagcctcgc gcatcggcga cctcgcgcgc atcaaggcga tgatcctcgt
     1861 ggacatgatc ggcgaccgcg atctgctcat caagcgcgag tcgaactcga ccgcgtggct
     1921 caccgatatc atctggtcgt cggcgaaggc gctggggttc ggcgcgcact tcgcccccga
     1981 cgccatctcc gtcgaagacg atcacattgc tttccttcag gcgggcgtcc cggcggtcaa
     2041 cctgatcgac atggacttcc cggcctggca tacgcccgac gacacgctgg acaaggtcag
     2101 cgcgaggagc atggaggttg tgggccgggt gctcgcggcc gccctgccga agatcgaagc
     2161 gcggctactc ggccgcgccc gctgagcggg cccggcgccg tcggccggcc agccacacgc
     2221 cgagcgtc
//
LOCUS       DAOSIF010000502         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42315_length_2221_cov_3.896122, whole genome shotgun sequence.
ACCESSION   DAOSIF010000502 DAOSIF010000000
VERSION     DAOSIF010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42315_length_2221_cov_3.896122"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="PLT35_13070"
     CDS             complement(<1..1493)
                     /locus_tag="PLT35_13070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG30202.1"
                     /translation="MMKLVKVTIDGIRTEAEDRTPLVQVAQKIGVKIPTLCHHPLVEP
                     YGVCRICTVEVHMRGRVRLVTACNYPVTDGIEVFTQTDRVRRTRKLILEWLLARCGHV
                     PLLNTLAAEYGITEPRFGRGDDTCILCGLCVRVCSDVVGAHALGFFNRGTTRYVGTPY
                     DEPSDRCIACGACVEVCPTGCITMDDEVEQIRGELPLGPMTAIYIPTMQAVPHQPVID
                     YDSCIHFKTDACRVCAAVCEAKAIDHGQQDRREEIQVGSIIVATGFQPVDPQKVPQYG
                     FGRYPNVVTALQVEHMVNASGPTNGQVLLKDGSRPQSVGIIHCVGSRDKNTHEYCSRV
                     CCMYSLKLAHLVHERSGAKVYNFYIDMRSAGKGYEEFYNHLLEEGCEFIRGRVAEVSD
                     WAVTPAEQGKLVIRVEDTLAGLVRRIPVDMVVLSTGLEAREDADEVRRLFNMSCSSEG
                     WFLERHPKLAPVSTFTDGVFIAGCAQGPKDIPDTVAQAGAAAAEALAL"
     gene            complement(1519..>2221)
                     /locus_tag="PLT35_13075"
     CDS             complement(1519..>2221)
                     /locus_tag="PLT35_13075"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG30203.1"
                     /translation="LIEVPMGITLREIIFGIGGGIPRGRAFKAVQTGGPSGGCLPATH
                     LDMPVDFDSLTAAGSMMGSGGMIVMDDQTCMVDVARYFIAFLTEESCGKCIPCREGLR
                     CSLDVLTRITEGKGEEGDIALLEEMGAVMQDACLCALGSSAPNPVMSTLRYFRDEYEA
                     HVRERRCPAGVCKALITYTIDQALCDGCHACVTACSTGAITGQVKYPHQLNQSLCIKC
                     GACLDACKRNAVLRA"
BASE COUNT          323 a          780 c          774 g          344 t
ORIGIN      
        1 agggccaggg cctcggcggc ggcggcgccc gcctgcgcga cggtgtccgg gatgtccttc
       61 gggccctggg cgcagccggc gatgaagacg ccgtcggtga aggtcgagac gggcgccagc
      121 ttcgggtggc gctccaggaa ccagccctcg ctcgagcacg acatgttgaa gaggcgccgg
      181 acctcgtcgg cgtcctcccg cgcctcgagg cccgtcgaga gcacgaccat gtccaccggg
      241 atgcggcgca cgaggccggc cagcgtgtcc tcgacgcgga tcaccagctt gccctgctcg
      301 gcgggcgtca ccgcccagtc cgagacctcg gcgacgcggc cgcggatgaa ctcgcagccc
      361 tcctcgagca ggtggttgta gaactcctcg tagcccttcc cggcgctgcg catgtcgatg
      421 tagaagttgt agaccttcgc gccgctgcgc tcgtggacga ggtgcgccag cttcagcgag
      481 tacatgcagc agacgcgcga gcagtactcg tgcgtgttct tgtcgcgcga gccgacgcag
      541 tggatgatgc cgaccgactg cggccggctg ccgtccttca gcagcacctg gccgttcgtc
      601 ggcccggacg cgttcaccat gtgctccacc tggagcgccg tgacgacgtt cgggtagcgg
      661 ccgaagccgt actgcggcac cttctgcggg tccacgggct ggaacccggt ggccacgatg
      721 atcgagccga cctggatctc ctcgcgccgg tcctgctgcc cgtggtcgat ggccttggcc
      781 tcgcacaccg ccgcgcacac ccggcaggcg tcggtcttga agtggatgca ggagtcgtag
      841 tcgatcaccg gctggtgcgg cacggcctgc atcgtcggga tgtagatggc cgtcatgggc
      901 ccgaggggca gctcgccgcg gatctgctcg acctcgtcgt ccatcgtgat gcagccggtg
      961 gggcagacct cgacgcacgc gccgcaggcg atgcaccggt cggagggctc gtcgtagggc
     1021 gtccccacgt agcgcgtcgt cccgcggttg aagaacccca gcgcgtgcgc cccgaccacg
     1081 tccgagcaga cgcgcacgca caggccgcac aggatgcagg tgtcgtcgcc gcggccgaac
     1141 cgcggctcgg tgatgccgta ctccgccgcc agcgtgttga ggagcgggac gtggccgcag
     1201 cgcgcgagca gccactcgag gatgagcttg cgcgtccggc ggacccggtc cgtctgcgtg
     1261 aacacctcga tgccgtccgt gacggggtag ttgcaggcgg tcaccaggcg gacgcggccc
     1321 cgcatgtgca cctccacggt gcagatgcgg cagacgccgt agggctcgac gagcgggtga
     1381 tggcagaggg tcgggatctt gacgccgatc ttctgggcca cctgcacgag gggcgtgcgg
     1441 tcttcggcct cggtcctgat gccgtcgatc gtgaccttca cgagcttcat catggcctcc
     1501 agcagcgagg ggccgggcct aggcccgcag caccgcgttg cgcttgcacg cgtccaggca
     1561 ggcgccgcac ttgatgcaca gcgactggtt cagctggtgc gggtacttca cctggcccgt
     1621 gatggccccg gtcgagcagg ccgtcacgca ggcgtggcac ccgtcgcaga gcgcctggtc
     1681 gatggtgtac gtgatgagcg ccttgcacac cccggccggg cagcggcgct ccctgacgtg
     1741 cgcctcgtac tcgtcccgga agtaccgcag cgtggacatc accgggttcg gcgccgacga
     1801 gccgagggcg cacaggcagg cgtcctgcat gacagccccc atctcctcga gcagggcgat
     1861 gtcgccctcc tcgcccttgc cctcggtgat gcgcgtcagg acgtcgaggg agcagcgcag
     1921 cccctctcgg cacgggatgc acttgccgca cgactcctcg gtcaggaacg cgatgaagta
     1981 gcgggcgacg tccaccatgc aggtctggtc gtccatcacg atcatcccgc ccgaccccat
     2041 catcgagccg gccgccgtca ggctgtcgaa gtccacgggc atgtccaggt gcgtcgccgg
     2101 caggcagccg cccgacggcc cgcccgtctg cacggccttg aaggcccgcc cccgcgggat
     2161 cccgccgccg atgccgaaga tgatctcgcg cagcgtgatt cccatcggca cctcgatgag
     2221 g
//
LOCUS       DAOSIF010000503         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42444_length_2216_cov_4.132809, whole genome shotgun sequence.
ACCESSION   DAOSIF010000503 DAOSIF010000000
VERSION     DAOSIF010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42444_length_2216_cov_4.132809"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PLT35_13080"
     CDS             complement(<1..1045)
                     /locus_tag="PLT35_13080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOG30204.1"
                     /translation="MLPWTPARLPSSVGWYAPNRAGLADGATIANLAALSGGGPALAG
                     TAVTVDASAALRGMPAAKFLAASSSALSASTGGVSSGAHTIAVIARFDEVPTQPADAA
                     YLGRQYPAALSAVGVTGSANGGKWWAGHTGSLDPRFQPAQDTQPHLFVKRSTGSTVDV
                     WIDGQHVLRASATTPAIGPGITLGGPWAAGFDGDKHLWAAAFSGAAIDLATISQIEAW
                     AHRECGLFRLILDGDSIAYGYGAASHGAPRDLLAGWTGTNTAAAVGAAYLPSCGLSTT
                     VAASGTTSTQILARQAAQVLPLLPGSDTSWVRGAGIPVWAVLSSGTNDLSQATSGNHA
                     VVTAAAIANAQAYC"
     gene            complement(1090..1575)
                     /locus_tag="PLT35_13085"
     CDS             complement(1090..1575)
                     /locus_tag="PLT35_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30205.1"
                     /translation="MATPRIWQAAKAALRELWPHALTILATDGGTNDGNGVEVTGTSA
                     GAVNVSAAQLPAALGPTTPALSTSVTGSLAATQASIDDVTARGSAAAFGSLAAGPGGI
                     VVANTSAPGGPNVRIDGAPTATHGVQLAPGEKWIFAVTNANQLKHIAESGSPTVSAAQ
                     A"
     gene            complement(1587..>2216)
                     /locus_tag="PLT35_13090"
     CDS             complement(1587..>2216)
                     /locus_tag="PLT35_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30206.1"
                     /translation="VYNPATGDVDVDVIGGGGVGVSGTASTSTGVQVPTTVMQVPAPD
                     NAGTSMRLTLKGWSLTGSGAAYGAEYVIHVGVVSGVLAAQMITRLSLLDTGRLTAQGG
                     VANCANNGSGFIRVTTDATHNLTTGMAVVIQGVTGTTEANGTWTITKIDSVTFDLQGS
                     TFVHAFVALGAWARGGGFLGISWSAGQLSIAVQGVGEMPIDWSARAQVF"
BASE COUNT          314 a          796 c          776 g          330 t
ORIGIN      
        1 cgcagtaggc ctgagcgttg gcgatggccg ccgcggtgac gacagcgtga ttgccgctcg
       61 tggcctggct caaatcgttg gtgccgctgg acagcacggc ccacaccggg atccccgctc
      121 cgcgaaccca gctcgtgtcc gagccgggga gcagcgggag cacctgcgcc gcctgtcgcg
      181 caaggatctg cgtgctcgtg gtgccgctgg ctgcgaccgt cgtggagagg ccgcagctcg
      241 gcaggtaggc cgcaccgacc gccgccgcgg tgttggtccc ggtccatccg gcgaggagat
      301 cgcgtggcgc gccgtgcgac gcggcgccgt agccgtaggc gatggagtcg ccgtcgagga
      361 tgagcctgaa gaggccgcac tcgcggtgcg cccacgcctc gatctgcgag atcgtcgcaa
      421 ggtcgatcgc ggcgccgctg aacgccgccg cccacagatg cttatccccg tcgaatcccg
      481 cggcccacgg cccgccgagc gtgatcccgg gcccgatcgc gggcgtcgtc gccgaggcgc
      541 ggagcacgtg ctgcccgtcg atccagacgt ccaccgtgct cccggtggag cgcttcacga
      601 acaagtgcgg ctgcgtgtcc tgcgcgggct ggaaacgcgg gtcgaggctg cccgtatgtc
      661 cggcccacca cttgccaccg ttggcgcttc cggtaacgcc gaccgcggag agcgcggcgg
      721 ggtactgcct cccgaggtac gcggcgtccg cgggctgcgt cggcacttca tcgaagcgag
      781 cgatcacggc gatcgtgtgc gcgcccgacg acacgccgcc cgtgctcgcg gaaagcgccg
      841 aggatgacgc cgccaagaac ttcgcggccg gcatgccacg gagcgccgcc gatgcgtcga
      901 cggtcaccgc ggtgccggcg agcgcgggac cgccgccgga gagcgcggca aggttggcga
      961 tcgtggcgcc atccgcaagc ccggcgcggt tgggcgcgta ccacccgacc gacgacggaa
     1021 gacgagcagg ggtccatggc agcacgtgcc cacgctttcg gcgcagcacc cgatcgagcc
     1081 ctcccgcgct catgcctgcg ccgcgctcac ggtgggcgag ccactctcgg cgatgtgctt
     1141 gagctggttc gcgttcgtga cggcgaagat ccacttctcg ccgggcgcga gttgcacgcc
     1201 gtgcgtcgcc gtgggcgcgc cgtcgatccg cacgttgggc ccgcccggcg cgctggtgtt
     1261 cgcgacgacg atgccgccag ggcccgccgc gagcgagccg aacgccgccg cgctgcccct
     1321 ggccgtcacg tcgtcgatcg atgcctgcgt cgccgcgagc gagccggtga cgctggtcga
     1381 gagcgcgggc gtcgtgggcc cgagcgcggc ggggagctgc gccgccgaca cgttgaccgc
     1441 gcccgcgctc gtgcccgtga cctcgacgcc gttgccgtcg ttggtgccgc cgtcggtagc
     1501 taggatcgtc agcgcatgcg gccagagctc gcggagagct gcctttgctg cctgccagat
     1561 acgaggggtc gccatgtcgg atcctcctag aaaacctgcg ctctagcgct ccagtcgatc
     1621 ggcatctccc cgactccctg aacggcgatc gacagctgcc ccgcggacca cgagataccg
     1681 aggaaaccgc ctccgcgcgc ccatgcgccc agcgcgacga atgcgtgcac gaacgtcgat
     1741 ccttgaaggt cgaacgtcac ggagtcgatc ttcgtgatcg tccacgtgcc gttggcctcc
     1801 gtggtgccgg tgaccccctg gatcacgacg gccatccccg tggtcaggtt gtgcgtcgcg
     1861 tcggtcgtta cgcggatgaa gccgctcccg ttgttggcgc agttggctac gcctccctgc
     1921 gcggtaagcc gccccgtgtc aagcagcgag agcctcgtga tcatctgcgc cgcgagcacg
     1981 ccagaaacga cgcccacatg gatcacgtac tcggccccgt aggctgcgcc gctccccgtc
     2041 aatgaccacc ccttcagggt gagtcgcatc gacgtcccgg cgttgtccgg cgcgggcact
     2101 tgcatgacgg tcgtcggaac ctgcacgccg gtggaagtgg atgcggtacc agaaacgccg
     2161 acgccgccac cgccgatcac gtcgacgtcg acgtcgcccg tcgcggggtt gtagac
//
LOCUS       DAOSIF010000504         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42895_length_2199_cov_3.518657, whole genome shotgun sequence.
ACCESSION   DAOSIF010000504 DAOSIF010000000
VERSION     DAOSIF010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42895_length_2199_cov_3.518657"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>2199
                     /locus_tag="PLT35_13095"
     CDS             31..>2199
                     /locus_tag="PLT35_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG30207.1"
                     /translation="MKRQTIVQVAALCAVAGATSVGMWAQPNKYARDPKQAVDAEYTQ
                     KIREYTTEPYYLSPLVDYLPASKTVPTPKAALGDVAGAPGILPYSAEVHEYMRMLEKA
                     SPRRVKVFSIGKSEEGREMIAVAIADDAVMAKFEENRARLAKLADPRTIAMNDAEADR
                     MVTASVPVYYITASLHAGETGSPTALMELAYRLVVDDSPYIKAIRSNLITIMTPVLET
                     DGRDRHVDVYRWHLANPTLVYPSLPYWGKYVLHDNNRDAMAATLMLTRNVIDFVVQQK
                     AQVLHDLHESVPYLYDNTVGDGPYNAWVDPILTDEWQLLGWNNMSELTRFGMPGAFAH
                     GTFDTWSPGYLMFIGAMHNGTSRLYETFGNAGADTVERTLQPDEYARTWYRQNPPLPR
                     TKWSQRNNNNYTITALLTALSYYGENNKLFLKNFYLKSKRSVLKPKAEGPAAYVFPAD
                     EARPGAQAELLRTLMRQAIEISRATAPFTVRMPVKKARPQGRGGDEGAGEGPKQPAAK
                     AGGPVEQKTDAKTGEEPKAETRTFPAGSYVVRMDQPYSRIADALLDYQYWSPNDPQKR
                     PYDDTGWTFGELFNVQVVRVTDATVLDAAMERVRDVAAPSDIKGSGPVFVVANDADPS
                     LFAFRYRLKTAGFAAAEEPFDAAGRSFPRGSFIVTGAAPADVQAACRELGVQAHAVAE
                     APAVKTHAVKTPRIGYVHTWIGTQDEGWWRLALDRLGIPYD"
BASE COUNT          406 a          740 c          756 g          297 t
ORIGIN      
        1 gtcccggccg gtcgggagcg agggtgatcg atgaagcgtc agacaatcgt acaggtggcg
       61 gcgctctgcg cggtggcggg cgcgacgagc gtcggcatgt gggcccagcc gaacaagtat
      121 gcgcgggacc cgaaacaggc agtggatgcg gagtacacgc agaagatcag ggagtacacc
      181 accgaaccgt actacctctc gccgctggtg gactacctgc cggcgtcgaa gaccgtgccg
      241 acgccgaagg ccgccttggg cgacgtcgcg ggcgcgccgg gcatcctgcc gtactcggcc
      301 gaggtgcacg agtacatgcg gatgctcgag aaggcgagcc cccggcgcgt caaggtgttc
      361 tccatcggga agtccgagga agggcgggag atgattgccg tcgccatcgc ggacgatgcc
      421 gtgatggcga agttcgagga gaaccgcgcg cggctggcga agctcgccga cccgcggacg
      481 atcgcgatga acgacgccga ggccgaccgc atggtcaccg cgtcggtgcc ggtctactac
      541 atcaccgcgt cgctgcatgc gggcgagacc gggtcgccca ccgcgctcat ggagctggcg
      601 taccggctcg tcgtggacga cagcccctac atcaaggcca tccgctcgaa cctcatcacg
      661 atcatgacgc ccgtcctcga gacagacggg cgcgaccgcc acgtggacgt gtacaggtgg
      721 cacctcgcga acccgacgct ggtctacccg tcgctgccgt actggggcaa gtacgtcctg
      781 cacgacaaca accgcgatgc gatggcggcc acgctgatgc tcacgcgcaa cgtgatcgac
      841 ttcgtcgtcc agcagaaggc gcaggtgctc cacgatctcc acgagtcggt gccctacctg
      901 tacgacaaca ccgtcggcga cgggccgtac aacgcctggg tggatcccat cctgacggac
      961 gagtggcagc tgctcggctg gaacaacatg tccgagctca cgaggttcgg gatgcccggg
     1021 gccttcgccc acggcacctt cgatacgtgg tcgccgggct acctgatgtt catcggcgcc
     1081 atgcacaacg ggacgagccg cctgtacgag accttcggca acgcgggcgc ggacaccgtg
     1141 gagcggacgc tccagcccga cgagtacgcc aggacgtggt accggcagaa cccgccgctg
     1201 cccaggacga agtggtcgca gcggaacaac aacaactaca cgatcaccgc gctgctgacg
     1261 gcgctgtcct actacggcga gaacaacaag ctcttcctca agaacttcta tctgaagagc
     1321 aagcgctcgg tgctcaagcc gaaggctgag ggcccggctg cgtacgtctt cccggcagac
     1381 gaagcccgcc cgggcgcgca ggccgaactg ctccgcacgc tgatgcgcca ggcgatcgag
     1441 atctcccgcg ccaccgcgcc gttcacggtc aggatgccgg tcaagaaggc gcggccccag
     1501 ggacggggcg gcgacgaggg agcgggcgaa ggcccgaagc agccggcagc gaaggccgga
     1561 ggaccggtcg aacagaagac ggatgcgaag accggcgagg agccgaaggc cgagacgagg
     1621 actttcccgg ccggcagcta cgtcgtccgg atggatcagc cgtacagccg gatcgcggac
     1681 gccctgctcg attaccagta ctggagcccg aacgatccgc aaaagcgtcc gtacgacgac
     1741 accggctgga cgttcggcga actgttcaac gtccaggtcg tccgggtgac cgatgcgacg
     1801 gtcctcgacg ccgccatgga gcgcgtgagg gacgtcgcgg ccccgtccga catcaagggg
     1861 agcggcccgg tgttcgtcgt cgccaacgac gcggacccgt cgctcttcgc cttccgctac
     1921 cggctcaaga cggcaggctt cgctgcggcg gaggagccgt ttgacgccgc cggccgctcg
     1981 ttcccccgcg gctcgttcat cgtcacgggc gcggcgcccg cggacgtgca ggcggcctgc
     2041 cgcgagctgg gcgtccaggc ccacgccgtc gccgaggcgc cggcggtcaa gacccacgct
     2101 gtgaagactc cgcgcatcgg gtacgtgcac acctggatcg gcacgcagga cgagggctgg
     2161 tggcggctgg ccctggacag gctgggcatc ccctacgac
//
LOCUS       DAOSIF010000505         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42957_length_2197_cov_3.393557, whole genome shotgun sequence.
ACCESSION   DAOSIF010000505 DAOSIF010000000
VERSION     DAOSIF010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42957_length_2197_cov_3.393557"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLT35_13100"
     CDS             complement(<1..1318)
                     /locus_tag="PLT35_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG30208.1"
                     /translation="MSVNRKKLIIGAVIVALAGALVAANVFLKREKGVEVDLEKLAAR
                     DLEAVVSASGKIQARTTVNISSQSMGRVTRLAVKEGDRVQRGQFLVEIDPRSLRSQVE
                     RGDAALSAQRVAVAQARTQLESAKTQLALARETLKRQQGLWDEQLTTREALDRAENEV
                     QVREREVEAREAAITMEATRVRQTAADLANARYNLGLLSIESPIDGIVTRRSIEIGEN
                     VMIGTMNNPGTVLMTIADMSIIEAELEVDETDIPTVALGQPAKVTIDALPDREFTGTV
                     TEIGNSPIQAAAAAAARATNFKVVVTIDGEIPEVRPGFTCTADITTATRKGVVSVPIQ
                     ATTVRELVYDEKGAVVKPPETGRKTRRAAAAAAPPDELKPGQTRKETEGVFAVKDGKA
                     VFKPVKTGIAGEKYLEALSGVAAGEEVIVGPFNSVRDLKDGDPVKVK"
     gene            complement(1329..2054)
                     /locus_tag="PLT35_13105"
     CDS             complement(1329..2054)
                     /locus_tag="PLT35_13105"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOG30209.1"
                     /translation="MTSAASATPHSPAGNPGLSLAARVWGILTSPRTTFVDVVARPRW
                     FGMMALVLVVTAACTGGFLSTEVGQTAWMDQAVERAQQSGRHLSDAELAATEKMVPYM
                     GIITAVSTLIIGPVMWLAMAGILFAVFNAALGGTSTFRQLFAVLVHSSVPTLLQQLIV
                     TPLNYARESMSSATSLAVFLPMLEEGSFPAKLLRMADLFVIWWIALLAIGLGVLFRRR
                     TGPIATGLFAVYGVIAVVLAAAF"
BASE COUNT          303 a          806 c          744 g          344 t
ORIGIN      
        1 tcttcacctt cacggggtcg ccgtccttca ggtcgcgcac cgagttgaac gggccgacaa
       61 tcacctcctc gcccgcggcg acgcccgaca gcgcctcgag gtacttctcg ccggcgatgc
      121 cggtcttgac cggcttgaag acggccttgc cgtccttcac cgcgaagacg ccctcggtct
      181 ccttcctggt ctgcccgggc ttgagctcgt cgggcggcgc ggcggccgcc gccgcccggc
      241 gcgtcttccg gccggtctcg ggcggcttca ccacggcgcc cttctcgtcg tagacgagct
      301 cgcggaccgt cgtcgcctgg atcggcaccg acacgacgcc cttgcgcgtg gccgtcgtga
      361 tgtcggccgt gcacgtgaag cccggccgca cctccgggat ctcgccgtcg atcgtcacga
      421 cgaccttgaa gttcgtggcg cgcgccgcgg cggccgcggc ggcctggatg gggctgttgc
      481 cgatttcggt gaccgtgccc gtgaactccc ggtccggcag cgcgtcgatg gtgaccttcg
      541 ccggctgccc cagcgcgacg gtggggatgt cggtctcgtc cacctcgagt tcggcctcga
      601 tgatcgacat gtcggcgatg gtcatcagga cggtgccggg gttgttcatc gtgccgatca
      661 tcacgttctc gccgatctcg atgctgcggc gcgtcacgat gccgtcgatg ggcgactcga
      721 tggacagcag gccgaggttg tagcgggcgt tggccaggtc ggccgcggtc tgccggaccc
      781 gcgtcgcctc catcgtgatg gccgcctcgc gcgcctcgac ctcgcgctcc cgcacctgga
      841 cctcgttctc ggcgcggtcg agggcctcgc gggtggtgag ctgctcgtcc cacagcccct
      901 gctggcgctt cagcgtctcc cgggccaggg cgagctgcgt cttggccgac tcgagctggg
      961 tcctcgcctg cgccaccgcg acccgctgcg ccgacagggc cgcgtcgccg cgctccacct
     1021 gcgaccgcag cgaccgggga tcgatctcga cgaggaactg gccccgctgc acgcggtcgc
     1081 cttccttgac ggccagccgc gtcacgcggc ccatcgactg cgagctgatg ttcaccgtcg
     1141 tgcgggcctg gatcttgccc gacgccgaca cgacggcctc caggtcccgg gccgcgagct
     1201 tctcgagatc gacctcgacg cccttttccc gtttgaggaa cacgttggcg gccaccagcg
     1261 cgcccgccag cgccacgatg accgccccga tgatgagctt cttgcgattc acgctcacgc
     1321 ccccgcccct agaaggccgc cgccaagacg acggcgatga cgccgtagac cgcgaacagg
     1381 ccggtcgcga tgggaccggt ccggcgcctg aacagcacgc ccaggccgat ggccagcagg
     1441 gcgatccacc agatcacgaa gaggtcggcc atcctgagga gcttcgccgg gaagctcccc
     1501 tcctccagca tgggcaggaa caccgccagg ctcgtcgcgc tcgacatcga ctcccgggcg
     1561 tagttgagcg gcgtcacgat cagctgctgg agcagcgtcg ggacggacga atgcaccagc
     1621 acggcgaaga gctgccggaa ggtggatgtg ccgccgagcg ccgcgttgaa caccgcgaag
     1681 aggatgccgg ccatggccag ccacatcacc ggcccgatga tgagcgtcga cacggccgtg
     1741 atgatgccca tgtagggcac catcttctcc gtcgcggcca gctcggcgtc cgacaaatgg
     1801 cggccggact gctgcgcgcg ctcgacggcc tggtccatcc aggcggtctg gccgacctcg
     1861 gtcgagagga acccgcccgt gcaggcggcg gtgaccacga gcaccagggc catcatgccg
     1921 aaccagcgcg gccgcgccac gacgtcgacg aaggtcgtcc tgggcgacgt caggatgccc
     1981 cagacacgtg ccgcgaggct caggcccggg tttccggcgg gcgaatgagg ggtggcagac
     2041 gctgcactcg tcatgtggac ctcgcatccc gcaaatcgcg cccccggcgg cgcgccacgg
     2101 ctttccgcgg ccgggcgggc ggcgcataac tctccaagtg tagacgattt ccggccgcgg
     2161 cgtgttccgg cgccgccgcc cgcggctgct ggccgga
//
LOCUS       DAOSIF010000506         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_42975_length_2196_cov_3.231667, whole genome shotgun sequence.
ACCESSION   DAOSIF010000506 DAOSIF010000000
VERSION     DAOSIF010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42975_length_2196_cov_3.231667"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLT35_13110"
     CDS             <1..734
                     /locus_tag="PLT35_13110"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG30210.1"
                     /translation="LYKRMRVGQFLAYMARLKSADGPGLQARIRQWLERIDLGGVEHK
                     RCEELSKGMQQKVQFLAAIVHRPALLVLDEPFAGLDPVNQRLLRELVTEEHRRGVTVL
                     FSTHIMAHAEQLCDHVVMIHRGEKVLDDRLSDIRARHVPRTVVFEPLDPAAATEALAR
                     VPGVARVSVTGRTWELALAPDAAPASVIQRAAAAIPPARVELTRPSLEDVFFRIVSAR
                     PAEHGGAPGAGAAGGSGAGPSEARP"
     gene            731..2131
                     /locus_tag="PLT35_13115"
     CDS             731..2131
                     /locus_tag="PLT35_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG30211.1"
                     /translation="MKKVIRVAKREFVATAGTKAFILGILVLPLVIGALMLVIPRMAM
                     EDPPAIEGRVAVIDPTGRVAAGLSAYLEPARIAQRRRQAFERMQEAMPPALRTASGMG
                     GRGGSAGRKVAGSLAGAVPRLEIVAIGAGIGVEQAKQALTAAAPQERPARPDLALVVV
                     HQDAVQRQPGADRFGTYDLFVRAKLDDRVIEEIQDGLRDSIVGARLSQAGLDRTQIDA
                     LTTVGRVKSRTVTAQGEGRTNEVLNRLIPMALMALLLMSVLTSGQYLLTTTVEEKTNR
                     VVEVLLSAVSPMELMAGKILGQFAVGLLMLALYLGLGLIALLTFASLGLLDPALILFL
                     AVFYLLAYFSLGAFMAAIGSAVNEMREAQGLMMPVMFVVMIPWLLWLPISRDPNSLLA
                     VALTFVPPLGSFVIMLRLASSTPPPLWQTLLAIAAGAAGVWASLWFAARVFRIGLLMY
                     GKPPSLATLVRWARSG"
BASE COUNT          290 a          771 c          800 g          335 t
ORIGIN      
        1 gcctctacaa gcgcatgcgc gtaggccagt tcctcgccta catggcccgc ctgaagagcg
       61 ccgacggccc cggcctccag gcgcgcatcc gccagtggct cgagcgaata gaccttggcg
      121 gcgtcgagca caaacgctgc gaggagctct cgaagggcat gcagcagaag gtgcagttcc
      181 tggcggccat cgtgcaccgc cccgccctgc tcgtcctcga cgagccgttc gccggcctcg
      241 atccggtcaa tcagcggctg ctgcgggagc tcgtgacgga ggagcaccgg cggggcgtca
      301 cggtgctgtt ctcgacccac atcatggcgc acgccgagca gttgtgcgac cacgtggtga
      361 tgatccaccg cggggagaag gtgctcgacg atcggctgag cgacatccgc gccaggcacg
      421 tgccccgcac cgtggtgttc gagccgctgg atcctgccgc agccaccgag gcgctcgcgc
      481 gggtgcccgg cgtcgcgcgc gtcagcgtga cgggccggac gtgggaactc gccctcgcac
      541 cggacgcggc gccggcctcc gtcatccagc gggctgcggc ggccatcccg ccggcccggg
      601 tcgaactcac gcggcccagc ctcgaggacg tcttcttccg catcgtcagc gccaggccgg
      661 ccgagcacgg cggggcgccc ggcgcggggg cggcgggcgg cagcggcgcg ggtccgtcgg
      721 aggcgcggcc atgaagaaag tcatccgcgt cgccaagcgc gagttcgtcg ccaccgcggg
      781 cacgaaggcg ttcatcctcg gcatcctcgt gctgccgctg gtcatcggcg ccttgatgct
      841 ggtcatcccg aggatggcca tggaggatcc ccccgccatc gaggggcgcg tggccgtcat
      901 cgacccgacc ggccgcgtcg ccgccggcct ctcggcctac ctcgagccgg cgcgcatcgc
      961 gcagcgccgc cggcaggcct tcgagcggat gcaggaggcg atgccgcccg cgctccggac
     1021 cgccagcggc atgggcggcc gcggcgggtc ggcaggccgg aaggtggccg gctcgctggc
     1081 cggcgcagtg cctcgtctcg agatcgtggc catcggcgcc ggaattggcg tcgagcaggc
     1141 caagcaggcg ctcacagcgg ccgcgccgca ggagaggccg gcccgacccg acctggcgct
     1201 cgtcgtcgtg caccaggacg ccgtgcagag gcagcccggc gcggatcgct tcggcacgta
     1261 cgatctgttc gtccgcgcga agctcgacga ccgggtcatc gaggagatcc aggacggtct
     1321 gcgcgactcg atcgtcggcg cgcggctcag ccaggccggc ctggacagga cgcagatcga
     1381 cgccctgacg acggtcgggc gggtgaagtc gagaaccgtg actgcgcagg gagagggccg
     1441 cacgaacgaa gtgctgaacc gcctgatccc gatggcgctg atggcgctgc tcctgatgtc
     1501 ggtgctcacg agcggccagt acctgctgac gacaaccgtg gaagaaaaga cgaaccgcgt
     1561 cgtcgaggtg ctgctgtcgg ccgtgtcgcc catggaactg atggctggga agatcctcgg
     1621 ccagttcgcc gtcggcctgc tcatgctggc gctgtacctg ggcctcggcc tgatcgccct
     1681 gctgacgttc gcctcgctcg gcctgctgga tccggcgctg atcctcttcc tcgcggtgtt
     1741 ctacctgctc gcctacttct cgctgggggc cttcatggcg gccatcgggt cggcggtgaa
     1801 cgagatgcgc gaggcgcagg gcctgatgat gccggtgatg ttcgtcgtca tgatcccgtg
     1861 gctgctctgg ctgcccatca gccgggaccc gaactcgctg ctcgccgtcg cgctgacgtt
     1921 tgtgccgccg ctcggcagct tcgtcatcat gctgcggctg gcgtcgtcca cgccgccgcc
     1981 gctctggcag acgctcctgg cgatcgccgc cggcgcggcc ggcgtgtggg cgtcgctctg
     2041 gttcgcggcg cgcgtgttcc gcatcggact gctgatgtac ggcaagccgc cgagccttgc
     2101 gacgctggtg cgctgggcgc ggagcggcta gccgggcggg gacgcgccgc cgtcggatgc
     2161 gtccggcggg agatccgcgt agtcctcggg ggtgtc
//
LOCUS       DAOSIF010000507         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_43487_length_2177_cov_4.920829, whole genome shotgun sequence.
ACCESSION   DAOSIF010000507 DAOSIF010000000
VERSION     DAOSIF010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43487_length_2177_cov_4.920829"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLT35_13120"
     CDS             complement(<1..936)
                     /locus_tag="PLT35_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG30212.1"
                     /translation="MKALVTGARGFIGTNLGVALERAGVEVARVDVGSTPGEWADGAR
                     GADAVFHLAGVNRPGDPSEFEAGNLGPLHALFAALDEAAAEGAATNLPAIVLSSSVQA
                     AEDNPYGRSKLAAELALEAYARRTGAPAVIYRLPGVFGKWCRPDYNSVVATFCHRVAR
                     GLPIAISDPARILRLVHVDDVIAGFAKHLETRPQGVTRAAAEPVFEISLGDLAASIRA
                     FRDSRTTLEAADASDPLTRRLLGTYASYIPPEDLAYPLEQRIDARGTLAELLKSARFG
                     QIFVSRTKPGVTRGNHYHDVKVEKFCVLEGEAAIRF"
     gene            complement(992..2017)
                     /locus_tag="PLT35_13125"
     CDS             complement(992..2017)
                     /locus_tag="PLT35_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOG30213.1"
                     /translation="MSVERTLLVTGGTGTFGHAVTGRALAMDFGEVRIFSRDERKQDD
                     MRRRFADSRVKFYLGDVRDERALTDAMNGVNVVFHAAALKQVPSCEFFPIEAVRTNTI
                     GAENTLNAAIAAGVSRVIVLSTDKAVYPINAMGMSKALMEKLAVAKSRVAGVSTVICC
                     TRYGNVMASRGSVVPLFIRQILAGAPLTVTDPNMTRFMMSIDGAVDLVFWAFDHGNPG
                     DTFVQKAPAATIETLALAMKRAFGAANPVSVIGTRHGEKLYETLLTREEMAVAEDMGD
                     YYRVPADGRDLNYGLYFDTGRRDVSAQEDYNSHNTRRLSVDELAAMLESLDIVKRALR
                     GEQVEEL"
BASE COUNT          307 a          763 c          757 g          350 t
ORIGIN      
        1 gaacctgatc gcggcttccc cctcgagcac gcagaacttc tccactttca cgtcgtggta
       61 gtggttgccc cgcgtcacgc cgggcttcgt cctcgacacg aagatctggc cgaagcgcgc
      121 tgacttcagc agctccgcga gggtgccgcg cgcgtcgatc cgctgctcca gcggataggc
      181 gaggtcctcg ggcgggatgt acgacgcgta ggtgccgagg agccgccggg tgagcgggtc
      241 ggaggcgtcg gcggcctcga gcgtcgttct ggagtcgcgg aaggcgcgga tgctggcggc
      301 caggtcgccg agagagatct cgaagacagg ctcggccgcc gcgcgcgtca cgccctgcgg
      361 ccgggtctcc agatgcttcg cgaagccggc aatcacatcg tccacgtgaa cgagcctgag
      421 gatgcgggcc ggatcggaga tcgcgattgg gagccccctg gcgacgcggt ggcagaaggt
      481 ggccacgacc gagttgtagt ccgggcggca ccacttcccg aagacgcctg gcaggcggta
      541 gatgacggcc ggcgcgccgg tgcgccgggc gtacgcctcc agggcgagtt cggctgccag
      601 tttcgagcgg ccgtacgggt tgtcctcggc cgcctggacg gaggacgaga ggacgatcgc
      661 gggcagattg gttgccgcgc cctccgcggc ggcttcgtcg agcgcggcaa agagcgcgtg
      721 aagcggcccg aggttgcccg cttcgaactc gctcgggtcc ccgggccggt tgacgccggc
      781 gagatggaag accgcgtcgg cgcccctggc cccatcggcc cactcgccgg gtgtcgagcc
      841 gacgtcgacg cgcgcgacct ccacgcccgc gcgctccagc gcgaccccga ggttcgtgcc
      901 gatgaagccg cgggcgccgg tgacgagggc cttcactgcg gcacctccgt ccgcgccgcc
      961 gggggcttcg ctccgccgcg cggcggctcc gtcacaactc ctccacctgc tcgcctctca
     1021 gcgcgcgctt cacgatgtcg agcgactcca gcatggcggc gagctcgtcc accgacagcc
     1081 ggcgcgtatt gtggctgttg tagtcctcct gcgccgacac gtcgcgccgg ccggtgtcga
     1141 agtacaggcc gtagttcaag tcgcgcccgt cggcgggcac ccggtagtag tcgcccatgt
     1201 cctcggcaac ggccatttcc tcgcgcgtga gcagcgtttc gtacagcttc tcgccgtgcc
     1261 gcgtgccgat cacgctgacc gggttggccg ccccaaacgc ccgcttcatc gccagggcca
     1321 gcgtctcgat cgtcgcggcg ggggctttct ggacgaaggt gtcgccgggg ttgccgtggt
     1381 cgaacgccca gaagacgagg tccaccgcgc cgtcgatgga catcatgaag cgggtcatgt
     1441 tcggatccgt gacggtgagg ggcgcgccag cgaggatctg gcggatgaac aacggcacga
     1501 ccgagccgcg gctggccatc acgttgccgt acctggtgca gcagatgacc gttgaaacgc
     1561 cggccacgcg cgatttcgcg acggccagct tctccatgag cgccttggac atgcccatcg
     1621 cgttgatcgg gtacacggcc ttgtcggtcg agagcacgat cacgcgcgac acgccggccg
     1681 cgatggccgc gttcagcgtg ttctcggcgc cgatggtgtt cgtgcgcacc gcctcgatgg
     1741 gaaagaactc gcacgagggc acctgcttga gcgcggcggc gtggaagacg acgttcacgc
     1801 cgttcatggc gtctgtcagg gcgcgttcgt cgcggacgtc gccaaggtag aacttcacgc
     1861 gggagtccgc gaagcgccgt cgcatgtcgt cctgcttccg ttcgtcgcgg ctgaagatcc
     1921 ggacctcccc gaaatccatc gccagcgccc gtccggtcac cgcgtgcccg aacgtgccgg
     1981 tgccgcccgt gacgagcaac gtgcgttcaa cggacatcag ccgcctcccc gttcgccttt
     2041 gccgcagccg cggcgaggta cgccgcgatc cgtcgtccct gcgccgtcca cgtcttctcg
     2101 gcgcgtatgt actcccgcgc ccgctgcccg acggccctcc gctcgccggc cggcattcgg
     2161 gccgcccgct gcatgag
//
LOCUS       DAOSIF010000508         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_43551_length_2175_cov_2.389623, whole genome shotgun sequence.
ACCESSION   DAOSIF010000508 DAOSIF010000000
VERSION     DAOSIF010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43551_length_2175_cov_2.389623"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="PLT35_13130"
     CDS             <1..1417
                     /locus_tag="PLT35_13130"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOG30214.1"
                     /translation="VSYGAPTEFRYNGRINEDEIDWSPERVANALTQDDLYVGMTLAE
                     AMDRLGFEATTAQYGDAFKDSQYDLWHANAGARRLLNLGIEAPWSGHPARNIHANDID
                     FQIEADFIGLMCPGLPRQSNAFCERVGRVMSHGDGLYGGMFVSGMYAAAYFEPDVRRV
                     VEGGLACLPAASAYARVVRDVLEWSAQHPDDWRRVWRLLQDRWDRDDGCPDGALRPFN
                     IDAKINGAYVALGLLYGRGDFGRTIEIATRAGQDSDCNPSTSGGILGVMLGYSGIPDV
                     WKSGIPAVAGETFQFTRSSLDDVCRSTVTRALRLVRLAGGSVGRDAVRVPFQPPEPPA
                     LEQWEMGVPERRIGVEDAAWRFTGFTDPGHGGPGGPTSRMQADGPGAEALLTFTGAAV
                     AITGDIGVDGGRADVYLDGAKAEPIDAYAGPRTHDNDLWHAYGLAQAPHTLRIVPRGE
                     ADPRSTGTRIAVTGAVVYRAR"
     gene            complement(1500..>2175)
                     /locus_tag="PLT35_13135"
     CDS             complement(1500..>2175)
                     /locus_tag="PLT35_13135"
                     /inference="COORDINATES: protein motif:HMM:NF023211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30215.1"
                     /translation="LLRRDYSQAGADRPAGVDRYGMPQYDKRLGTLTWGKNIVPKYLW
                     SDGTRNASLVGGTIAPSAPVVLNAPVGEKRDPKARIFPFQLHEAVQPYDTVNNVLALP
                     KLLGGYWVDYDWSKAISEGMKLVNVPYSGSYGFVETRMYSSVHHQVVAAGKALGCADC
                     HEPEAITCTRCHRNAAGMALPEHRRAMYPEAKARMDFKSLGYTDDPARVGGRFYVTLG
                     RGVPPR"
BASE COUNT          315 a          756 c          775 g          329 t
ORIGIN      
        1 cgtctcgtac ggcgcgccga cggagttccg gtacaacggg cgcatcaacg aagacgagat
       61 cgactggtcc ccggagcgcg tggcgaacgc gctgacgcag gacgatctgt acgtgggcat
      121 gacgctcgcc gaggcgatgg atcgcctcgg cttcgaggcc acgacggcgc agtacggcga
      181 cgcgttcaag gacagccagt acgatctgtg gcacgcgaac gcgggcgcca ggcgccttct
      241 gaacctcggc atcgaagcgc cgtggtccgg ccatccggcg cgcaacatcc acgcaaacga
      301 catcgacttc cagatcgagg ccgacttcat cggcctgatg tgcccggggc tgccgcgcca
      361 gtccaacgcg ttctgcgagc gggtcggccg ggtcatgagc cacggcgacg ggctctacgg
      421 cggcatgttc gtcagcggca tgtacgcggc cgcctacttc gaaccggacg tccggcgcgt
      481 cgtggaaggg ggcctcgcgt gcctgccggc cgcgagcgcg tacgcgcgcg tggtgcggga
      541 cgtcctcgag tggtcggcgc agcatccgga cgactggagg cgggtctggc ggctgctgca
      601 ggaccgctgg gaccgcgacg acggctgtcc ggacggggcg cttcggccct tcaacatcga
      661 tgcgaagatc aacggcgcgt acgtcgcgct gggcctgctc tacggtcgcg gcgacttcgg
      721 gcgaaccatc gagatcgcga cccgggccgg ccaggactcc gactgcaacc cctcgacctc
      781 cggcggcatc ctgggcgtca tgctcggcta ctcgggaatt ccggatgtct ggaagagcgg
      841 catcccggct gtcgccggcg aaacgtttca attcacgcgg tcgtcgctcg acgacgtctg
      901 ccgttcgacg gtgacgcggg cattgagact ggtccgcctg gccggcggct cggtcgggcg
      961 ggacgccgtc cgggttccct tccagccgcc ggagccgccg gcgctcgaac agtgggaaat
     1021 gggcgttccc gagcggcgaa ttggcgtcga ggacgccgcc tggcggttca ccgggttcac
     1081 ggacccgggt cacggcggcc ctggcggccc gacgtcgcgc atgcaggcgg acggacccgg
     1141 cgcagaggcc ctgctcacct tcaccggcgc ggctgtcgcc atcaccggcg acatcggggt
     1201 ggacggcggg cgcgccgacg tgtatctcga cggcgcgaag gcggagccga ttgacgccta
     1261 tgccggcccc cggacgcacg acaacgacct gtggcacgcg tacggcctgg cgcaggcgcc
     1321 tcacacgctg aggattgtcc cgcggggcga ggccgatccg cgatcgaccg gcacgcgcat
     1381 tgcggtgacc ggggccgtgg tctaccgggc caggtagcgc cgactcgggc ccgccgcaag
     1441 cccggccgcc gccgggcgcg ccctgcgccg gccgggcctc gctcgagtgg aggcgtcttc
     1501 tatcgcggcg gcacaccgcg gccgagcgtc acgtagaacc tgccgcccac gcgggccgga
     1561 tcgtccgtgt agccgaggct cttgaagtcc atgcgcgcct tcgcctccgg gtacatggcg
     1621 cgccggtgct ccgggagggc catgccggct gcgttccggt ggcaccgcgt gcacgtgatg
     1681 gcctcgggct cgtggcagtc ggcgcagccg agcgcttttc ctgccgccac gacctgatgg
     1741 tgcaccgagg agtacatcct ggtctcgaca aagccgtacg acccggaata cggcacgttc
     1801 accagcttca tgccttccga gatcgccttc gaccagtcgt agtccaccca gtacccgccc
     1861 agcagcttcg gcagcgcgag cacgttgttg accgtgtcgt acggctgcac ggcttcgtgc
     1921 agctggaacg ggaagatccg cgcctttgga tcgcgcttct cgcccaccgg cgcattcagg
     1981 accacgggcg ccgacggcgc gatggtcccg ccgacgagcg aggcattccg cgtgccgtcg
     2041 gaccacaggt acttgggcac gatgttcttg ccccacgtca gggtccctaa ccgcttgtcg
     2101 tactggggca tgccgtagcg gtccacgccg gcgggccggt cggcgccggc ttgcgagtag
     2161 tcgcgcctca ggagg
//
LOCUS       DAOSIF010000509         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_43660_length_2170_cov_3.632151, whole genome shotgun sequence.
ACCESSION   DAOSIF010000509 DAOSIF010000000
VERSION     DAOSIF010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43660_length_2170_cov_3.632151"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLT35_13140"
     CDS             complement(<1..194)
                     /locus_tag="PLT35_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30216.1"
                     /translation="MGAPSPGAGDETQWRSRRRAAAAAWAAFAFVVWNVVFDAAVLES
                     GRDYLTRQARHQQGQGPAVT"
     gene            complement(181..507)
                     /locus_tag="PLT35_13145"
     CDS             complement(181..507)
                     /locus_tag="PLT35_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30217.1"
                     /translation="MTAVRLRLEARPGGAHRDRDSAEKARPKQERAIPAPIRQALMPG
                     EERAGAFFSAAGARIPRRAIGCGCFDVVDLGDGRFGRAPASGRDTADAARNGAMTGPV
                     LRHGRA"
     gene            complement(504..785)
                     /locus_tag="PLT35_13150"
     CDS             complement(504..785)
                     /locus_tag="PLT35_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30218.1"
                     /translation="MTCRQCGAEISDKAIVCYRCGQATADTAASRAPARRAARLGWAE
                     ALTGLLALIAAALLMSRAAAGQLPAPVSYAVAALAAIVLIWRIARRRRR"
     gene            824..1408
                     /locus_tag="PLT35_13155"
     CDS             824..1408
                     /locus_tag="PLT35_13155"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG30219.1"
                     /translation="MIPIRDVVPTRTRPAATRALAATMAAALAWPAVRRGWLPWCATV
                     LACWIFGRTVEDRFGHGRFALLAAAGAAAALAAAWRLGEAPAPGLAAAGAAAGVMAAY
                     FLLFPRSKILTLVPVLVGVELVDVPAWVIAGLWALIHAADLWARAAFAGRPQAAALAG
                     AIAAGAAAGVIGWILLRRPERLSVEWWDTPSKRP"
     gene            complement(1471..>2170)
                     /locus_tag="PLT35_13160"
     CDS             complement(1471..>2170)
                     /locus_tag="PLT35_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30220.1"
                     /translation="LGAAGAFYHVARRQYWRTAAFAFLCGLARPHGFLLALPLWLAVF
                     WPALVESRSAGVSVLGRRLVGALRASAPAALASGAAVAGVLLFSGYVAALTGRPFTWL
                     EAHQAWGRVFGAWLSEGPIQEISYRGVCGYVLAQPIDALNLSAAVGCLAAIWPVTKRL
                     GPACGALVAVLLLPPLAAGGVISIGRITSVGFPVFIWLAGAIPVRSRAPWIAVFAMLQ
                     GWGASLFFTLRGFI"
BASE COUNT          306 a          842 c          777 g          245 t
ORIGIN      
        1 atggtcaccg cgggcccctg cccctgctga tgccgcgcct gccgtgtcag gtagtctcgc
       61 ccggactcga ggaccgcggc gtcgaagacg acgttccaga cgacgaacgc gaacgccgcc
      121 cacgcggccg ccgccgcgcg gcggcgcgac cgccactgcg tctcgtcgcc cgcgccgggg
      181 ctaggcgcgc ccatgccgca ggacgggccc ggtcatggcg ccgttccgcg ccgcatccgc
      241 cgtgtcccgc cccgacgccg gcgcccggcc gaaacgcccg tccccgagat cgacaacatc
      301 gaagcagccg cacccgatgg cgcgacgcgg gatgcgtgca ccggccgcgc tgaagaaggc
      361 gcccgcgcgc tcctccccag gcattagcgc ctgccggatg ggcgcgggga tcgcgcgctc
      421 ctgcttcgga cgcgccttct cggccgaatc gcggtcgcga tgcgcgcctc cgggccgggc
      481 ctccagcctg aggcggaccg cggtcatctt cgccggcgcc tggcgatgcg ccagatgagc
      541 acgatggccg ccagcgcggc cactgcgtag gacacgggcg cgggcagctg gccggccgcc
      601 gcgcgtgaca tgagcagggc tgccgcgatg agcgccagca gccccgtcag cgcctcggcc
      661 catccgagcc gcgccgcacg gcgggccggc gcgcgggaag ccgcagtgtc ggccgtcgcc
      721 tgcccgcagc ggtagcagac gatggccttg tcggagatct cggcgccgca ctgccggcaa
      781 gtcacgccat ggccctcgga agcgacgatt atatactgcg cgcatgatcc cgatccgcga
      841 tgtcgtgccg acgcgcacgc ggccagccgc gacgcgcgcg ctcgcggcca ccatggcggc
      901 ggcgctggcg tggccggcgg tccgccgggg ctggctcccc tggtgcgcga cggtgctggc
      961 ctgctggatc ttcgggcgga ccgtcgagga tcgattcggc cacggccgct tcgcgctgct
     1021 cgcggccgcc ggcgcggcgg ccgccctcgc ggcggcgtgg cgcctcgggg aggcgcctgc
     1081 accgggcctc gcggccgccg gcgcggcagc cggcgtgatg gccgcctatt tcctgctgtt
     1141 cccgcgatcg aagatcctca cgctcgtccc ggtcctcgtc ggcgtggaac tcgtggacgt
     1201 cccggcgtgg gtcatcgcgg gcctgtgggc cctcatccac gccgcggacc tgtgggcccg
     1261 cgccgccttc gcaggccggc cgcaggcagc ggcgctggca ggcgcgatcg cagcaggcgc
     1321 cgccgcaggg gtgatcgggt ggatcctgct ccgccgtccc gaacgcctca gcgtcgaatg
     1381 gtgggacacc ccatcgaagc ggccctgact caccacgctc gttgcgtgcc tggcccctcg
     1441 cccccgagtc ccgagtccgg agtccctgcc ctagatgaac ccgcgcagcg tgaagaacag
     1501 cgacgcgccc cagccctgaa gcatcgcaaa gacggcgatc cacggcgccc ggctcctgac
     1561 cgggatcgcg ccagcgagcc agatgaagac cggaaagccc accgacgtga tgcggccgat
     1621 cgagatgact cctcccgccg cgagcggcgg gagcagcagg accgccacca gcgcgccgca
     1681 ggctgggccg aggcgcttcg tcaccggcca gatcgcggcc aggcagccca ccgccgccga
     1741 gagattcagc gcgtcgattg gctgcgcgag cacgtatccg cagacgccgc ggtacgaaat
     1801 ctcctggatc gggccctccg agagccaggc gccgaagacg cggccccagg cctggtgcgc
     1861 ctcgagccag gtgaacggac gtccggtgag cgccgcgacg taccccgaaa acagcagcac
     1921 ccctgccact gcggctcccg atgccagcgc ggccggcgcc gaggcccgca gcgcgccgac
     1981 gagccggcgg ccgaggacgg agacgcccgc cgaccggctc tcgaccagcg ccggccagaa
     2041 gaccgcgagc cacagaggaa gcgcgaggag gaacccgtga gggcgcgcaa gcccgcacag
     2101 gaaggcgaat gctgcggtcc gccagtactg ccgccgcgca acgtggtaga acgctccggc
     2161 cgcgcccagc
//
LOCUS       DAOSIF010000510         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_43692_length_2169_cov_3.426679, whole genome shotgun sequence.
ACCESSION   DAOSIF010000510 DAOSIF010000000
VERSION     DAOSIF010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43692_length_2169_cov_3.426679"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1007)
                     /locus_tag="PLT35_13165"
     CDS             complement(54..1007)
                     /locus_tag="PLT35_13165"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOG30221.1"
                     /translation="MSDTATRLRGKDLLGIAGLTAEEITLILDTAEAMKEVSARPIKK
                     VPALRGKTVINLFYEPSTRTRTSFEVAEKRLSADTLSIAIASSSVVKGETLIDTALNL
                     EAMNPDIVVMRHASSGACHLLARTCQAGIVNAGDGMHEHPTQALLDAFTIRERKGRLK
                     GLKVAITGDLLHSRVFRSNAYLLTTMGAEVWACGPPTLMPAGIARLGVRPTTRVEEAV
                     EGADAVMMLRIQLERMQGGFFPSLREYFQVFGMTPARVRLAKPDAIIMHPGPMNRGVE
                     IESEVADGPASVILDQVANGVAVRMAVLYLLAGGSDDETAA"
     gene            complement(1004..1552)
                     /gene="pyrR"
                     /locus_tag="PLT35_13170"
     CDS             complement(1004..1552)
                     /gene="pyrR"
                     /locus_tag="PLT35_13170"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOG30222.1"
                     /translation="MPILMDGERVGRTLTRIAHEIVERNRGVGDLALVGVRTRGVPIA
                     RRLAAILREITGVDVPTGALDITLYRDDLMKSAVGPQPIVRRTEIPFSIDGRHIVLVD
                     DVLYTGRTIRAALDALIDFGRPSTIQLVVLVDRGHRELPIKADYVGKNVPTSRREIVS
                     VRLAEVDGADEIVLGQVEGAGA"
     gene            complement(1907..>2169)
                     /locus_tag="PLT35_13175"
     CDS             complement(1907..>2169)
                     /locus_tag="PLT35_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30223.1"
                     /translation="RLLERAVGNARLSGTHEPAPDGFLFAFGTDVRPGRYARGSVADL
                     TPTVLYFFGLPVGRDMDGFARPDIFAPEFTGRRPITYIPSYE"
BASE COUNT          314 a          812 c          738 g          305 t
ORIGIN      
        1 ggacgtcgcg cgccccgtcg acgcccgacg cgggatcgac gacccgcgcg cccctaagca
       61 gcagtctcat cgtcgctccc tcccgcgagc agatacagca ccgccatgcg cacggccacg
      121 ccgttggcca cctggtcgag aatgacggac gccgggccgt cggccacttc ggactcgatc
      181 tcgacgcccc ggttcatcgg gcccgggtgc atgatgatgg cgtccggctt cgccaggcgc
      241 acgcgcgcgg gcgtcatgcc gaagacctgg aagtactcgc gcagcgacgg gaagaaccct
      301 ccctgcatcc gctcgagctg gatgcgcagc atcatcaccg cgtcggcgcc ctccaccgcc
      361 tcctccaccc gcgtcgtggg ccgcacgccc agccgggcga tgcccgccgg catgagcgtc
      421 gggggcccgc acgcccacac ctccgcgccc atcgtcgtca gcaggtacgc gttcgagcgg
      481 aacacgcggc tgtgcagcag gtcgccggtg atggcgacct tgagcccttt cagccgtccc
      541 ttgcgctcgc ggatcgtgaa cgcgtcgagc agggcctgcg tgggatgctc gtgcatgccg
      601 tcgccggcat tgacgatgcc cgcctggcac gtgcgcgcca gcaggtggca cgccccggac
      661 gaggcgtgcc gcatgacgac gatgtccggg ttcatcgcct ccaggttcag cgcggtgtcg
      721 atcagggtct cgcccttcac cacgctcgac gacgcgatcg cgatgctgag cgtgtccgcg
      781 ctcaggcgct tctcggccac ctcgaacgac gtgcgcgtcc gcgtggacgg ctcgtagaac
      841 aggttgatga cggtcttccc gcgcagcgcg ggcaccttct tgatcgggcg cgcggacacc
      901 tccttcatcg cctccgccgt gtcgaggatg agggtgatct cctccgccgt caggccggca
      961 atgccgagca ggtccttgcc gcgaagccgc gtggccgtgt cgctcatgcc cccgctcctt
     1021 cgacctggcc cagaacgatc tcatcggccc cgtcgacctc cgccaggcgc acgctcacga
     1081 tctcgcgccg cgaggtgggc acgttcttgc cgacgtagtc ggccttgatc ggcagctcgc
     1141 ggtgcccgcg atcgacgagc accacgagct ggatcgtgct cggccggccg aagtcgatca
     1201 gcgcatcgag ggccgcccgg atcgtccgcc cggtgtacag cacgtcgtcc accagcacga
     1261 tgtgccggcc gtcgatcgag aagggaatct ccgtccggcg gacaatcggc tgcggcccga
     1321 cggcggactt catcaagtcg tcgcggtaca gcgtgatgtc gagggcgccg gtcggcacgt
     1381 cgacgcccgt gatctcgcgc aggatcgccg ccagccgcct ggcgatgggc acgccgcggg
     1441 tccggacgcc gacaagcgcc aggtcgccga cgccgcggtt gcgctcgacg atttcgtggg
     1501 cgattcgcgt gagcgtccgc ccgacgcgct cgccgtccat caggatgggc acgcggacct
     1561 ccaggtgggg gccacgggct gggcgccagg cgcgagccgc ccgcgcttcg ccgccgcgac
     1621 acgtgaacag gaagaacagg aatcccgctg cggcacgcaa cgggcgcacg gaggcaggga
     1681 ccgcatggag cctctctttg cggtctctcg gaccgcgatt aaagagccgg ctcagtgtag
     1741 cagaagagca ggaagcccgg aagcggccgg ccggagccgg aatgcggatc ccggagccct
     1801 cggccccctc cgctgacggg cgcccggccc gcagccctcg caccccggcc cgcgggcccc
     1861 cgtccggccg gcgcccgggg ccccggcccc cgccgccccg cctcgctcat tcgtaggacg
     1921 gaatgtaggt gatcggcctt ctccccgtga actccggcgc gaagatgtcc ggccgggcga
     1981 agccgtccat gtcccgcccc accggcagcc cgaagaagta gagcaccgtg ggggtcaggt
     2041 ccgcgaccga tccgcgggcg tagcggccgg gacggacgtc cgtgccgaac gcgaacagga
     2101 agccgtcggg cgccggttcg tgcgtgccgc tcagccgcgc attgcccacc gcgcgctcga
     2161 gcagccgct
//
LOCUS       DAOSIF010000511         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_43694_length_2169_cov_3.301798, whole genome shotgun sequence.
ACCESSION   DAOSIF010000511 DAOSIF010000000
VERSION     DAOSIF010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43694_length_2169_cov_3.301798"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLT35_13180"
     CDS             complement(<1..315)
                     /locus_tag="PLT35_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30224.1"
                     /translation="MRKAPLVLLAITLASVASPAAAGTPGRFMQYPDISGNAIVFSWD
                     RDLWSVPASGGVATRLTGHPGVENVPKFSPDGTQIAFAGQYEGNNLYVMPATGGAPRR
                     ITF"
     gene            complement(403..>607)
                     /locus_tag="PLT35_13185"
     CDS             complement(403..>607)
                     /locus_tag="PLT35_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30225.1"
                     /translation="RPLLLGEFPTRGSSRRAAEILAAARLAGYAGAFAWSALAGDGAS
                     DPAAMASALASLRTADAGPAARA"
     assembly_gap    608..617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<618..1338)
                     /locus_tag="PLT35_13190"
     CDS             complement(<618..1338)
                     /locus_tag="PLT35_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOG30226.1"
                     /translation="MRWPRALSFVVGTNLPWLRYGGDFGANAWRPDGGVGRRDAVPEL
                     RRTLARLADDGFTHVRWFMLCDGRAGLRPAPGGAPAGLDEFVFRDADAAVDALERAGL
                     RAIIVLFDFHWCLPAAVAGGVQTGGRRESIADPHARARLLEGVVAPILDRYGRHPAVA
                     AWDVMNEPEWVTRGGGGRASAGRVPREAMRAFLGGVVALVHERTAHAATIGSASTRTL
                     PLVEGLGLDVYQAHWYDRLDRR"
     gene            complement(1362..1538)
                     /locus_tag="PLT35_13195"
     CDS             complement(1362..1538)
                     /locus_tag="PLT35_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30227.1"
                     /translation="MRNYCWALIGLAALAFVVGTILAFTKGDFILVPQGYWRGAVGFL
                     LFAIALRLMDRPKA"
     gene            complement(1700..>2169)
                     /locus_tag="PLT35_13200"
     CDS             complement(1700..>2169)
                     /locus_tag="PLT35_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30228.1"
                     /translation="SYEHPVLVTGGLPWERRLELAAALFRDVPGVNRCLWNLGPRAPE
                     RIVPVAATVTRARLDVLREADHLVMDGLRRHGVHDRIWQCPTVLLPLEVDGAGREFVV
                     LRPVHSERAMTATPAELPEALLRELRAQVLALPGVSGLGLDVTTKPPGTIEWE"
BASE COUNT          327 a          820 c          765 g          247 t
ORIGIN      
        1 gaaggtaatc cgccgcggcg cgccgcctgt ggccggcatc acgtagaggt tgttgccctc
       61 gtactggccc gcgaacgcga tctgcgtgcc gtcgggcgag aacttcggga cgttctccac
      121 tcccgggtgg cccgtcagcc gcgtcgcgac gccgccggag gccgggacgc tccagaggtc
      181 gcggtcccag ctgaacacaa tcgcgtttcc cgagatgtcg gggtactgca tgaagcggcc
      241 gggcgtgccc gcagcggcgg gcgaggcgac cgacgccagg gtgatcgcga ggagcacgag
      301 cggggctttg cgcatggagg gactccagga aggcagtacc aggccgcccg atcttacacc
      361 cgccgggccg gccgcgggcg atgcgcgccg ccggtgcagc cgtcaggcgc gcgcggccgg
      421 gcccgcgtca gcggtgcgga gcgaggcgag cgccgaggcc atcgccgccg gatcggacgc
      481 gccgtcaccg gccagggccg accacgcgaa cgcgccggcg tagccggcca ggcgcgccgc
      541 ggcgaggatc tcggccgccc gacgcgacga cccgcgcgtc gggaactcgc cgagcagaag
      601 cggacgannn nnnnnnncgc ggcgatcgag ccggtcgtac cagtgggcct ggtagacgtc
      661 gagtcccagg ccctcgacga gcgggagcgt gcgcgtcgac gccgagccaa tcgtcgccgc
      721 gtgcgcggtg cgctcgtgca cgagcgccac gacgccgccg agaaacgcgc gcatggcctc
      781 gcgcggcacg cggcccgcag atgctcggcc gccgccgcct cgcgtgaccc actccggctc
      841 gttcatcacg tcccacgccg ccacggccgg gtgccggccg taccggtcga ggatcggcgc
      901 caccaccccc tcgagcaggc gggcgcgggc gtgcggatcg gcgatcgact cacggcggcc
      961 gccggtctga acgccgcccg cgacggcggc cggcaggcac cagtggaaat cgaacaggac
     1021 aatgatggcg cggagaccgg cccgctcgag agcgtcgacg gcggcgtcgg cgtcgcggaa
     1081 cacgaactcg tccagccccg cgggagcgcc gccgggcgcg ggccgcaggc ccgcgcgccc
     1141 gtcgcacagc atgaaccagc ggacgtgcgt gaacccgtcg tcggccagcc gggcgagcgt
     1201 ccgcctcaac tccggcacgg cgtcgcggcg cccgacgccg ccgtcgggac gccaggcgtt
     1261 ggcgccgaag tcgccgccgt agcgcagcca cggcaggttc gtgccgacga cgaaggagag
     1321 ggcccgaggc cagcgcatgc ccgcgcgcaa cgaggcccgc gctatgcctt cggacggtcc
     1381 atcagcctca gggcgattgc aaacagcagg aaacccaccg ccccgcgcca gtagccttgc
     1441 ggcacgagga tgaagtcgcc cttcgtgaac gccaggatgg tgccgacgac gaacgccagc
     1501 gccgcgagtc caatcaacgc ccagcagtag tttctcatcg ccgctctctc ctgtgtctcc
     1561 gagttcctgc ctctacagaa cggcccgccg tctacgcgca gctaatccga atgccgattg
     1621 gcagcagggc tctcgtatcc tcctttcccc tcaccccgcg cccctggcgt ctggccccga
     1681 gccccgagca cagatccccc tactcccact caatcgtccc cggcggcttc gtcgtgacgt
     1741 cgaggccgag cccgctcacg ccaggcagcg cgagcacctg tgcccggagc tctcgaagga
     1801 gcgcctcagg cagctcggcg ggagtcgccg tcatggcccg ctccgaatgg accggccgca
     1861 gcaccacgaa ctcccgcccg gcgccgtcca cctcgagcgg aagcaacacc gtcgggcact
     1921 gccagatccg gtcgtggacg ccgtggcggc gaagcccgtc catcaccagg tggtcggctt
     1981 ctctcaggac gtccaggcgc gcgcgcgtga ccgtcgccgc caccgggacg atgcgctccg
     2041 gggcccgggg cccgaggttc cacaggcagc ggttcacgcc cggcacgtcc cggaacaggg
     2101 cggcggccag ctcgaggcgg cgctcccacg gcaggccgcc ggtgacgagg accgggtgct
     2161 cgtacgacc
//
LOCUS       DAOSIF010000512         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_43918_length_2161_cov_3.908357, whole genome shotgun sequence.
ACCESSION   DAOSIF010000512 DAOSIF010000000
VERSION     DAOSIF010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43918_length_2161_cov_3.908357"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="PLT35_13205"
     CDS             complement(<1..1210)
                     /locus_tag="PLT35_13205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG30229.1"
                     /translation="MRILFVAPYVPSRVRARSYEFVRALTGLGHRVHVVALRPPEDRW
                     ADDAPVREACERMDVFPLSRVRTLANAGLALAGPGPLQEAYSRHPAARAHVARLAASR
                     LYDIAHVEHLRGATLLRGVAGLPVVFDAVDSISYLFEQAARLAVRRLQRVLARLELAR
                     TARFERQAPHRFARTVVTSPVDRDAIIRLAGPDAAHRVAVVPSIVDGSYFTPGRSAAG
                     QATVLFTGKMSYHANEAAAQHLVLRVMPEVWRSRPDARVIVAGKDPSPRILALADDPR
                     VQVTGYVADLRPLFARATVAVSPLLYGAGLQFKILEAMASGVPVVTSLKACASLTARP
                     EHELLAGETPEEIASQALRVLRDRKLAGRLGEAGREYVVREHNATVIGRRLTEIYGAA
                     REDSVPRPVRR"
     gene            complement(1215..>2161)
                     /locus_tag="PLT35_13210"
     CDS             complement(1215..>2161)
                     /locus_tag="PLT35_13210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG30230.1"
                     /translation="SIAGAGDLSGSASASVAMVSALTGAGTIGADGVGAAILASALVG
                     AGSVSADAIGAFLAAAGLTGAGTVAVTLTAPASIAAPLTGAGALAVSLYAQGNMTAAL
                     GQDVTEVSPDAIRTLADYLDGHPDTGICGPRIVFPDGTFQSCGYRFPTVWSEIRQSKN
                     VNRLVKALVGPETFPADWPEARDVDWVDGACLMIRREVIDRIGPLDEQFFLYAEELDW
                     CFNARKAGWRVAAVPAAGIVHHQGKSSAQVSDRSLEYFMDTRLRYYRKNRGLAVALFV
                     SAVYGAGCLKQWRSEPRKSRVKLRAVSRWWAGLFGRAR"
BASE COUNT          328 a          781 c          766 g          286 t
ORIGIN      
        1 cgcgccgaac cggacgcgga acggagtcct cgcgcgcggc gccgtagatc tccgtcaggc
       61 gccggccgat gaccgtcgcg ttgtgctccc gcacgacgta ctcgcggccc gcctcgccca
      121 gccgcccggc caacttcctg tcgcgcaaga cacgaagggc ctggctggcg atctcctcgg
      181 gcgtctcgcc cgccagcagt tcgtgttcgg gccgggccgt caggctcgcg cacgccttca
      241 gggaggtcac gaccggcact cccgacgcca tggcctcgag gatcttgaac tgcagcccgg
      301 cgccgtacag cagcggcgag accgccacgg tggccctggc gaagagcggc cggaggtcgg
      361 cgacgtagcc cgtgacctgg accctcgggt cgtctgcgag cgcgaggatt cgcggggagg
      421 ggtctttgcc tgccacgatc accctggcgt cgggccggct gcgccacacc tccggcatga
      481 cgcgcaggac gaggtgctgt gccgccgcct cgttggcgtg atagctcatc ttgccggtga
      541 agagcacggt cgcctggccg gcggccgatc ggcctggcgt gaagtacgac ccgtccacga
      601 tgctcgggac gacggcgacg cggtgcgcgg cgtccggccc tgcgagccgg atgatcgcgt
      661 cacgatcgac cggcgacgtc acgacggtgc gcgcgaaccg atgaggggcc tgccgctcga
      721 agcgcgccgt gcgcgcgagt tcgagcctcg ccagaacccg ctgcaggcgc cggacggcga
      781 gcctggcggc ctgctcgaac aggtagctga tggaatcgac cgcgtcgaag accaccggaa
      841 ggccggccac gcctctcaac agcgtcgcgc cccggaggtg ctcgacgtgc gcgatgtcgt
      901 agaggcgcga ggcagccagc ctcgccacgt gggcgcgcgc ggcgggatgc cgggaatagg
      961 cttcctggag cgggccgggt cccgccagcg cgagcccggc attggcgagg gtccgaaccc
     1021 gcgacaaggg aaacacgtcc attcgctcgc acgcctccct cacgggcgcg tcgtcggccc
     1081 agcggtcctc gggcgggcgg agcgcgacga cgtgaacccg gtgtccgagc cccgtcagcg
     1141 cacggacgaa ctcgtacgat cgggcgcgga cccgcgacgg cacgtagggc gcgacgaaga
     1201 ggatccgcat ggcgtcaccg ggcgcggccg aagaggcccg cccaccacct cgagacggcc
     1261 cgcagtttca ccctcgactt gcggggctcg ctgcgccact gcttcaggca gccggcgccg
     1321 tagaccgcgc tcacgaagag cgccacggcc aggccacggt tcttgcggta gtacctgagc
     1381 cgcgtgtcca tgaagtactc gagcgaacgg tcgctcacct gggcgctgct cttcccctgg
     1441 tgatggacga tcccggcggc tggcaccgca gccacgcgcc agccggcctt ccgggcgttg
     1501 aaacaccagt cgagttcctc ggcgtacagg aagaactgct cgtcgagcgg cccaatccga
     1561 tcgatgacct cccgccggat catcaggcat gcgccgtcca cccagtccac gtcacgggcc
     1621 tcgggccagt cagcggggaa ggtctccggg ccgacgagcg ccttgacgag gcgattgacg
     1681 ttcttcgact ggcggatttc ggaccacacc gtcgggaacc ggtagccgca cgactggaag
     1741 gtcccgtcag ggaagacgat cctcgggccg cagatgcctg tgtcggggtg cccgtcgaga
     1801 tagtcggcca acgttcggat ggcgtcgggg ctcacctcgg tgacgtcctg cccgagcgcc
     1861 gccgtcatgt tgccctgcgc gtagagcgac accgccagcg cgcccgctcc ggtcagtggc
     1921 gccgcgatgc tggccggggc cgtgagcgtg accgcgacag tccccgcgcc cgtgagtccg
     1981 gctgcggcga ggaacgctcc gatcgcatcg gccgacacgg agcccgcgcc gacgagtgcg
     2041 cttgcgagga tcgcagcgcc gacgccatcg gccccgatcg tgcccgcgcc cgtcagagcc
     2101 gacaccatcg cgaccgacgc agacgccgag ccggagaggt cgcccgcgcc cgcgatcgag
     2161 g
//
LOCUS       DAOSIF010000513         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_43976_length_2159_cov_4.518536, whole genome shotgun sequence.
ACCESSION   DAOSIF010000513 DAOSIF010000000
VERSION     DAOSIF010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43976_length_2159_cov_4.518536"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /locus_tag="PLT35_13215"
     CDS             complement(<1..1917)
                     /locus_tag="PLT35_13215"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOG30231.1"
                     /translation="MSGEVKAGQDTSRAPSSQRIQQVAAPPDVLQMIGGQITVTIDGT
                     DVKVPMGTTVLEAARQIGIRIPTLCHHPDLCVAGVCRICVVEVEGQRTLQAACAYPIT
                     GSLKVKTHTRKVRQARRHILDLLLSTHYGECYSCKRNNNCELQSLAKEYGVDFYRFGH
                     VTESQYPKDESSYSVIRGMDKCILCRRCVRTCIDVQEVGTLEAVGRSNRTRISTFLDK
                     PLAGVVCINCGQCINRCPTGALHANDTSDEVWRAIDDPTKHVVIQTAPSPRAGIGECF
                     GCEPGTPLTFQLNTALRLCGFDKVFDTNFSADLTIIEEGTELLGRLYRALVKQEPAAF
                     PQFTSCSPGWVKYLEHFYPEYIPNLSSAKSPQQMFGAVIKTYYAKTHNIDPKDIVTVA
                     LMPCSAKKFECNRPEMAGSGFKDVDYGLTTRELAQMIMEAGIHLPDMPKSGFDDPFGD
                     RTGSGVIFGATGGVMEAALRTVLELVTGRKVEKLFEHANIVPVRGFDGVRYVELPITQ
                     VADEVPALIRHVIPTFEFLKGATLKLAVAHGTANAHKVLEDIKAGGKLSECHFIEFMA
                     CPGGCIGGGGQPIPTSPEIRAARARAIYAEDMESDVRKSHENPAVARLYSEFFTDGPC
                     GHLAHDLLHTHYTAR"
BASE COUNT          302 a          726 c          741 g          390 t
ORIGIN      
        1 cctcgccgtg tagtgcgtgt ggagcaggtc gtgcgcgagg tggccgcacg ggccgtcggt
       61 gaagaactcg ctgtagagcc tcgcgacggc cgggttctcg tgggacttcc gcacgtccga
      121 ctccatgtcc tcggcgtaga tcgcccgggc ccgcgccgcg cggatctcgg gcgaggtcgg
      181 gatcggctgt ccgccgccgc cgatgcagcc gcccgggcag gccatgaact cgatgaagtg
      241 gcactcgctg agcttcccgc cggccttgat gtcctccagg accttgtggg cgttcgccgt
      301 gccgtgggcg acggccagct tcagcgtcgc gcctttcagg aactcgaacg tcgggatgac
      361 gtgccggatg agggcgggca cctcgtcggc aacctgcgtg atcggcagct cgacgtaccg
      421 cacgccgtcg aacccgcgca cggggacgat gttggcgtgc tcgaagagct tttcgacctt
      481 ccgtccggtc accagctcga gcacggtccg gagggccgcc tccatgacgc ccccggtcgc
      541 gccgaagatc acgcccgacc cggtccggtc gccgaaggga tcgtcgaagc cgctcttcgg
      601 catgtccggg aggtggatgc cggcctccat gatcatctgg gccagctccc gggtcgtcag
      661 gccgtagtcg acgtccttga acccgctgcc ggccatctcc ggccggttgc actcgaattt
      721 cttcgctgag cagggcatca gggcgacggt gacgatgtcc ttcggatcga tgttgtgggt
      781 cttggcgtag taggtcttga tgaccgcgcc gaacatctgc tgcgggctct tggccgacga
      841 caggttgggg atgtactcgg gatagaagtg ctcgaggtac ttcacccatc ccggcgagca
      901 gctggtgaac tgcgggaacg ccgccggctc ctgcttcacg agcgcccggt acaggcggcc
      961 gagaagctcc gtgccctcct cgatgatggt caggtcggcg ctgaagttcg tgtcgaacac
     1021 cttgtcgaag ccgcagagcc tgagggcggt gttcagctgg aacgtcaggg gcgtgccggg
     1081 ctcgcacccg aagcactcgc cgatcccggc cctcgggctc ggcgccgtct ggatgacgac
     1141 gtgcttggtc gggtcgtcga tggcgcgcca gacctcgtcg gacgtgtcgt tggcgtgcag
     1201 cgcgcccgtc gggcaccggt tgatgcactg gccgcagttg atgcagacga cgccggcgag
     1261 gggcttgtcg aggaaggtcg agatgcgggt ccggttgctg cgccccaccg cctcgagcgt
     1321 gcccacctcc tgcacgtcga tgcaggtgcg gacgcaccgg cggcacagga tgcacttgtc
     1381 catgccgcgg atgacggagt agctcgactc gtccttcggg tactgcgact cggtgacgtg
     1441 gccgaagcgg tagaagtcga cgccgtactc cttggccagc gactgcagtt cgcagttgtt
     1501 gttgcgcttg cagctatagc actcgccgta gtgcgtcgac agcagcaggt cgaggatatg
     1561 gcgcctcgcc tgccgcacct tccgcgtgtg cgtcttcacc ttcagcgacc cggtgatcgg
     1621 gtaggcgcag gcggcctgga gcgtccgctg cccttcgacc tcgacgacac agatgcggca
     1681 gacccccgcg acgcacaggt cgggatggtg gcagagcgtc gggatgcgga tcccgatctg
     1741 ccgcgccgcc tccagcacgg tcgttcccat cgggaccttg acgtccgtcc cgtcgatggt
     1801 gaccgtgatc tggccgccga tcatctgcag cacgtccggc ggcgcggcca cctgctggat
     1861 gcgctggctg ctcggcgcgc gcgacgtgtc ctgtccggcc ttcacttctc cgctcatcgt
     1921 tcacctcggc accgggcgcc agtcccggtg gcatcgcttg cgtcaacggc tcccgtcccg
     1981 ccggcgcggc cgcctcagcg gtccgccggc gtccggccca ggatctcgtc ctggaaattc
     2041 gtcacgatcg acaggaaggc gttcgggctc gactggccca ggccgcactt cgacgcgacc
     2101 tgcatcgtct cgcagagcct gacgagctcg ttcaggtagg ccgacgagca cgcgcctcg
//
LOCUS       DAOSIF010000514         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44133_length_2153_cov_3.897521, whole genome shotgun sequence.
ACCESSION   DAOSIF010000514 DAOSIF010000000
VERSION     DAOSIF010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44133_length_2153_cov_3.897521"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLT35_13220"
     CDS             <1..1042
                     /locus_tag="PLT35_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30232.1"
                     /translation="RPVVYGMATICEAFTDGWGRVLRAPAILIGVFLVSLLLAAPAGS
                     AIRDSISGSLGSSLDGGAMADGVDADWWARFSDAATGLDRTFTRSVIGFAAVLDNLSR
                     FLDNEEADASLAGLTFAYLAIWLFLIGGILDRYARMRPTRSQAFFGACGTLFFRFLRL
                     GLIAAAGYWLLFGVVHAWLFEGLYGRAIRDLTVERTAFAIRLALYGVFLALLGFWNAV
                     MDYAKIRAVVEDRRSMLGAFLAGWRFVVGHPLKAGGLYLLNTLAFAAVVFVYFLTAPG
                     AAGSGAAAWAAFAIGQAYVLARLAVKLAFYASGTSLFQRSLAHLAYTAAPEPIWPDSP
                     AAEALANAAGRD"
     gene            1053..1817
                     /gene="nadE"
                     /locus_tag="PLT35_13225"
     CDS             1053..1817
                     /gene="nadE"
                     /locus_tag="PLT35_13225"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOG30233.1"
                     /translation="MHHLASEMAGWLRERVRAAGARGIVVGLSGGLDSAVVARVAQMA
                     VPGGVLTVFMPAGSLPQDAEDARLVEDTFGLPVMTVDLSDAVDALLDRLHHAAGAQPF
                     DTAPADEAGPRMAVANLKPRLRMAALYYVANRLNYLVAGTGNRCEIAIGYYTKHGDGG
                     VDLLPLGALVKSEVRQLARHLGVPARIIDKEPSAGLWPGQTDEGEMGFTYAELEDYLR
                     GRRASLAPATLARIERLVRASEHKRQMPPVFRPSGE"
     gene            complement(1830..>2153)
                     /locus_tag="PLT35_13230"
     CDS             complement(1830..>2153)
                     /locus_tag="PLT35_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30234.1"
                     /translation="ASDGAAAQGVPADVIVVNGFPLDDGFLGGLAARYERMVTVEDGL
                     IGDPSSGTRGFAGLVATSVAGAGVRLDHFGIGDPRIAPSDHFVRVWEHFGLTEDAMLA
                     ALVRA"
BASE COUNT          264 a          773 c          788 g          328 t
ORIGIN      
        1 ccggccggtg gtatacggca tggcgacgat ctgcgaggcg ttcacggacg ggtgggggcg
       61 ggtcctccgc gcgccggcca tcctgatcgg cgtgttcctc gtcagcctgc tcctggccgc
      121 gccggccggg agcgccatcc gggactcgat ctcaggcagc ctgggctcga gcctcgacgg
      181 cggcgccatg gccgacggcg tcgacgccga ctggtgggcc cggttcagcg acgcggccac
      241 gggcctggat cggacgttca cccggtccgt catcggcttc gccgccgtgc tcgacaacct
      301 cagccgcttc ctggacaacg aggaggcgga cgcgtcgctg gccgggctca cgttcgccta
      361 cctggcgatc tggctgttcc tcatcggcgg cattctcgac cggtacgcgc ggatgaggcc
      421 gacgcggtcc caggcgttct tcggcgcctg cgggacgctc ttcttccgct tcctccgcct
      481 cgggctgatc gcggccgccg gctactggct gctgttcggc gtcgtgcatg cgtggctgtt
      541 cgagggactc tacgggcggg ccatccgcga cctgacggtg gagcggacgg cattcgcgat
      601 ccgcctggcg ctctacggcg tgttcctggc gctgctgggg ttctggaacg ccgtcatgga
      661 ctacgcgaag atccgcgcgg tggtggagga ccgccggagc atgctcgggg cgttcctcgc
      721 gggctggcgc ttcgtcgtcg gccatccgct gaaggccggc ggcctgtacc tgctcaacac
      781 gctcgccttc gcggcggttg tgttcgtgta cttcctgacg gcgccgggcg cggccgggtc
      841 tggcgccgcc gcgtgggcgg cgttcgccat cgggcaggcg tacgtgctgg cgcggcttgc
      901 cgtgaagctc gccttctacg cctccgggac ttcgctcttc cagcggtccc tcgcccacct
      961 ggcgtatacc gcggcgccgg agccgatctg gcccgactcg cccgcggccg aggccctcgc
     1021 caacgccgcc ggcagggact aggagcggcc cgatgcacca cctggcgtcc gagatggccg
     1081 gctggctccg ggagcgcgtg cgcgcggcgg gcgcgcgggg gatcgtcgtg gggctcagcg
     1141 ggggactgga ctcagccgtc gtcgcccgcg tggcgcagat ggccgtgccc ggcggcgtgc
     1201 tgacggtgtt catgcccgca ggcagcctgc cgcaggacgc cgaggacgcg cggctcgtcg
     1261 aggacacgtt cggcctgccc gtcatgacgg tcgatctctc ggacgcggtt gacgcgctgc
     1321 tcgatcgcct gcaccacgcg gccggcgcgc agccgttcga cacggccccg gcagacgagg
     1381 cagggccgcg catggccgtg gccaatctga agccgcgcct gcgcatggcg gcgctctact
     1441 acgtcgcgaa ccggctgaac tacctcgtcg ccggcacggg caaccggtgc gagatcgcca
     1501 tcggctacta cacgaagcac ggcgatgggg gtgtggatct gctcccgctc ggggcgctcg
     1561 tgaagagcga ggtgcgccag ctcgcgcgcc acctcggcgt gccggcccgg atcatcgaca
     1621 aggagccgag cgcgggcctc tggccgggcc agaccgacga gggcgagatg gggttcacct
     1681 acgccgagct ggaagactac ctccgcggga ggcgggcttc gcttgctccg gccacgctgg
     1741 cgcgcatcga gcgcctcgtg cgcgccagcg agcacaagcg ccagatgccg ccggtgttcc
     1801 ggccctccgg ggagtaggca gcgggcccct caggcccgga cgagcgccgc gagcatggcg
     1861 tcttcggtca agccgaagtg ctcccacacc cggacgaagt ggtccgaggg tgcgatgcgc
     1921 ggatcgccga tcccgaagtg atccagccgg acgcccgcgc cggccacgct cgtggcgacg
     1981 agccccgcga agccgcgtgt ccccgacgag ggatcgccga tcagtccgtc ttcgaccgtc
     2041 accatgcgct cgtacctggc ggccagcccg ccgaggaacc cgtcgtccag cgggaatccg
     2101 ttgacgacga tgacgtccgc cggcacgccc tgcgcggccg cgccgtcgga cgc
//
LOCUS       DAOSIF010000515         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44162_length_2152_cov_5.870291, whole genome shotgun sequence.
ACCESSION   DAOSIF010000515 DAOSIF010000000
VERSION     DAOSIF010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44162_length_2152_cov_5.870291"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..637
                     /locus_tag="PLT35_13235"
     CDS             179..637
                     /locus_tag="PLT35_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30235.1"
                     /translation="MPRSDRDDRRPRIVTFLAAVNAAGFATTLLFGGLVSVRRLVPLP
                     GSLTAAADRANAAVTYGFMIGDVLYSAPLLLAAAVGLWRLRSWGWLAAQIVNALWIYS
                     MTVILLRDAYTRLSPGGVLFLPFAVVSIWAIPRLWQCRAMFGVDAGPVWT"
     gene            642..1127
                     /locus_tag="PLT35_13240"
     CDS             642..1127
                     /locus_tag="PLT35_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30236.1"
                     /translation="MRAFLVGMTCAAAMMAGCGSDSPAAPDTDDPETLKAAPLSVVFA
                     GKTLVLSASLWRDFQPISPPDGRALAGVLRVTATDGSGVPASVTADSAWLVLGSQVWQ
                     TTPAEIGSRDGSAPVYELAVRDGPKWGPGVSVDVVVRLRDGSGRAALLRAADQVIQRT
                     D"
     gene            complement(1167..>2152)
                     /locus_tag="PLT35_13245"
     CDS             complement(1167..>2152)
                     /locus_tag="PLT35_13245"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOG30237.1"
                     /translation="VFTVAVSSRTTTPVMLTEVEYDGVVGFGEASMPPYLGESHETAA
                     AFLSKVDLAPFANPLELETILAAVDALAPGNPAAKASVDIALHDLAGKLMGQPWYNIW
                     GYDPARAPLTTFTIGIDTPEVVRQKTREAAEFKVLKIKLGRDTDKAMVEAIRSVTDKP
                     LTGDANQGWTDRQQALDMIGWLKERGFEYVEQPLAKERVDDLAWITERSPIPIIGDEG
                     VQRLADVRKAHGVYSGLNIKLMKSTGMREAHRMLELARALGMKVMIGCMTETSCAISA
                     AAQLSPMADWADLDGALLISNDVFDGATVVDGKVRIPDRPGIGAIRKAVTG"
BASE COUNT          271 a          759 c          761 g          361 t
ORIGIN      
        1 ctggtgggtg cagatcagga acgaacaggg acaggccggc tggaccagcg agccggagaa
       61 gttcgacggg aagagcgcag tcggccgccg agcgcgtcga acgacgaatg ccggctgcct
      121 tcggcgccgg cgcgatgggc tcggcggttg ggccgcttgc accgggggag ggggctgcat
      181 gccgcgttcc gaccgggacg atcgccggcc gcgcatcgtg acgttcctgg ccgccgtcaa
      241 tgcggcaggc ttcgccacga cgctgctctt cggggggctc gtgtccgtca ggcggctcgt
      301 gccgctccca ggcagcctga ccgccgcggc cgaccgggcc aatgccgccg tgacgtacgg
      361 cttcatgatc ggcgatgtgc tgtactcggc gccgctgctc ctcgccgcgg ccgtcggcct
      421 ctggcggctc cggagctggg gctggctggc ggcgcagatc gtgaacgcgc tgtggatcta
      481 ctcgatgacg gtcatcctct tgcgggacgc gtacacccgg ttgtcgccgg gcggcgtgct
      541 cttcctcccg ttcgccgtcg tctccatctg ggcgatcccg cgcttgtggc agtgccgggc
      601 gatgttcggc gtcgacgccg ggccggtctg gacgtgattc gatgcgcgcc ttcctggtgg
      661 gcatgacgtg cgcggcggcc atgatggccg gttgcgggag cgattcgccc gccgcgcccg
      721 atacggacga tcccgagacg ctgaaggccg caccgctcag cgtcgtcttc gccggcaaga
      781 cgcttgtcct gtcggcctcg ctctggcgcg acttccagcc aatttcgccg cccgacggca
      841 gggccctcgc gggggtgctt cgcgtcacgg caacggacgg ctccggcgtg cccgccagcg
      901 tcacggccga cagcgcctgg ctggtcctgg gctcgcaagt gtggcagacg acgcccgctg
      961 agatcggcag ccgggacgga agtgcgcccg tctacgaact cgccgtgcgc gacgggccga
     1021 aatggggtcc cggcgtgtca gtcgacgtcg tcgtccggct gcgcgacggc agcgggcgcg
     1081 cggcgctgct gcgggcggcc gaccaggtga tccagcggac cgactgaggg cccggcgcct
     1141 gtccggggcg attcgcctgc gcccgttcag cccgtgacgg ccttcctgat cgcgccgatg
     1201 cccggccggt ccgggatgcg cacctttcca tccacgaccg ttgcgccatc gaacacgtcg
     1261 ttcgagatga gcagcgcgcc gtcgaggtcc gcccagtccg ccatcggcga gagttgcgcc
     1321 gcggccgaga tggcgcacga cgtctccgtc atgcagccga tcatcacctt catgccgagg
     1381 gcgcgggcga gctccagcat cctgtgcgcc tcgcgcatcc cggtggattt catcagcttg
     1441 atgttgaggc ccgagtagac gccgtgcgcc ttccgcacgt ccgcgagccg ctgcacgccc
     1501 tcgtctccga tgatggggat ggggctgcgc tcggtgatcc acgccaggtc gtccacgcgc
     1561 tccttcgcga gcggctgttc gacgtactcg aacccgcgct ccttcagcca gccgatcatg
     1621 tcgagggcct gctgccgatc cgtccagccc tggttggcgt cgcccgtcag cggcttgtcc
     1681 gtcaccgagc ggatcgcctc gaccatcgcc ttgtccgtgt cgcggcccag cttgatcttc
     1741 aggaccttga actcggccgc ctcgcgggtc ttctgccgca ccacctccgg cgtgtcgatg
     1801 ccgatcgtga acgtcgtcag gggcgccctg gccgggtcgt agccccagat gttgtaccag
     1861 ggctgtccca tgagcttgcc cgccaggtcg tggagggcga tgtccaccga ggccttcgcc
     1921 gccgggttgc cgggggcgag cgcgtcgacg gccgcgagga tcgtctcgag ctcgaggggg
     1981 ttcgcgaagg gggccagatc caccttcgac aggaacgccg cggccgtctc gtgcgactcg
     2041 ccgaggtagg ggggcatcga ggcctcgccg aagccgacca cgccgtcgta ctccacctcc
     2101 gtcagcatca ccggcgtcgt cgtccgggag ctcaccgcca ccgtgaagac gt
//
LOCUS       DAOSIF010000516         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44382_length_2144_cov_3.999043, whole genome shotgun sequence.
ACCESSION   DAOSIF010000516 DAOSIF010000000
VERSION     DAOSIF010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44382_length_2144_cov_3.999043"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PLT35_13250"
     CDS             <1..1037
                     /locus_tag="PLT35_13250"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG30238.1"
                     /translation="GKVATVVPFVDDSFVGLAGGGSVRDLETILQLVHLHFTRPRADA
                     TAFAVLKSQLRTIVANRRESPERLFEDAAATALSQDHFRRRPLTEAVVEEMSLERSMR
                     FFKERFADAGGFTFVFSGSFDVQAVKPLVARYLASLPASGRKETWKDPGIRPPRGVVQ
                     RVVRKGLEPKSRVRLVFTGAFPYEPAERVALRVLGLVLEGQLGSVLREDQSGTYGVKV
                     ETDRQKIPNPEYALSVDFACEPARADDLVARVFAEIGRLKTSDLSDYYVRTVREALLR
                     EYETDSKENRYMVEQIAEACDNGEDPAVVIKQPEIFRRVTATTIQVAARRYLDTRNYV
                     RITLVPEAPK"
     gene            complement(1048..>2144)
                     /locus_tag="PLT35_13255"
     CDS             complement(1048..>2144)
                     /locus_tag="PLT35_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG30239.1"
                     /translation="DAADPAVQAWEALMWKYQRALPGAPPGSKWQLLDRIFDLPAQAP
                     EPAPQPRPGGGSAMSRRIVVATEKPFAKVAVSGIAGIFRDAGYQCRWLERYTDKADYV
                     RAVADADGLIVRSDVVDAEVLDAAPALKIVVRAGAGYDNIDLKAASARGVVAMNTPGQ
                     NSNAVAELVFALMLTLARRGFTGKDGTELRGKTIGIHAYGHVGARVAAIARGFGMEAC
                     AYDPYVPAARIEADGVTPLSSADELYRLSDYISLHLPKTKDTVQSVGYALLAQAKPTA
                     TLVNTARAEVVDEAGLMQVMSERQGFLYGADVAPARAAEFTEKFPGRCVFTAKKMGAQ
                     TAEANINAGLAAARQIVGFFERGDATFRVN"
BASE COUNT          313 a          739 c          762 g          330 t
ORIGIN      
        1 cggggaaggt cgcgaccgtc gtccccttcg tcgacgactc gttcgtgggg ttggcgggag
       61 ggggctccgt gcgcgatctg gagacgatcc tccagctggt gcatctccac ttcaccaggc
      121 ccagggccga cgcgacggcg ttcgcggtgc tgaagagcca gctgcggacg atcgtggcga
      181 accggcgcga gtcgccggaa cggctgttcg aggacgcggc ggcaaccgcg ctgtcgcagg
      241 accacttccg acggcggccc ctgaccgagg cggtggtcga agagatgagc ctcgagcggt
      301 ccatgcggtt cttcaaggag cggttcgcgg acgcaggggg cttcaccttc gtcttctcgg
      361 gcagcttcga cgtccaggcc gtgaagccgc tcgtcgcgcg ctacctggcg tcgctgcccg
      421 cctcgggtcg gaaggaaacg tggaaggacc cgggcatccg ccccccgcgg ggcgtcgtgc
      481 agcgcgtcgt gcggaagggc ctcgagccca agagccgcgt ccgcctggtg ttcaccgggg
      541 cgttcccgta cgaaccggcc gagcgcgtcg ccttgcgggt gctggggctg gtgctggagg
      601 gacagctggg ctcggtgctc cgcgaagacc agtccgggac ctacggagtg aaggtggaga
      661 cggaccgcca gaagatcccg aacccggagt acgccttgtc cgtcgatttc gcgtgcgagc
      721 cggcccgggc cgacgatctc gtggcccggg tcttcgccga gatcggccgc ctgaagacca
      781 gcgacctgtc ggactactac gtcaggaccg tgcgcgaggc cctgctgcgg gagtacgaga
      841 ccgattcgaa ggagaaccgc tacatggtcg agcagatcgc agaggcctgc gacaacggcg
      901 aagacccggc cgtcgtcatc aagcagccgg agatcttccg ccgcgtgacg gccacgacga
      961 ttcaggtcgc ggcgcggcgg tatctcgaca cgcggaacta cgttcggatc accctcgtcc
     1021 cggaggcgcc gaagtagcgc cgcgcgctca gttcacgcgg aacgtcgcgt cgccgcgctc
     1081 gaagaagccg acgatctgcc gcgcggccgc caggccggcg ttgatgttgg cttcggccgt
     1141 ctgcgctccc atcttcttcg ccgtgaagac gcagcggccg gggaacttct cggtgaattc
     1201 ggccgcccgc gccggcgcga cgtcggcgcc gtacaggaag ccctggcgct cggacatcac
     1261 ctgcatcagg ccggcttcgt ccacgacctc ggcccgggcg gtgttgacca gcgtcgccgt
     1321 cggcttggcc tgcgccagca gggcgtagcc caccgactgc acggtgtcct tcgtcttcgg
     1381 caggtgcagg gagatgtagt ccgagaggcg gtagagctcg tcggccgacg agagcggcgt
     1441 cacgccgtcg gcttcgatcc tcgccgccgg gacgtaggga tcgtaggcgc acgcctccat
     1501 gccgaagccc ctggcaatcg ccgcgacacg ggcgccgacg tggccgtagg cgtggatgcc
     1561 gatcgtcttg ccgcgcagtt ccgtcccgtc cttgccggtg aagccgcggc gggccagcgt
     1621 gagcatcaag gcgaagacca gctcggcgac ggcattcgag ttctggcccg gcgtgttcat
     1681 ggccacgacg ccccgggcgc tcgccgcctt cagatcgatg ttgtcgtacc ccgcgccggc
     1741 ccgaaccacg atcttcagcg ccggcgcagc gtcgagcacc tcggcgtcca cgacgtcgct
     1801 gcgcacgatc aggccgtcgg cgtccgcgac ggcccgcacg tagtcggcct tgtccgtgta
     1861 gcgctccagc caccggcact ggtagcccgc gtcgcggaag atgccggcga tgccggacac
     1921 ggccaccttc gcgaacggtt tctctgtcgc cacgacgatc cttctggaca tcgcgctgcc
     1981 tcctccgggg cggggctggg gggccggctc gggagcctgc gccggcagat cgaagatcct
     2041 gtcgaggagc tgccacttgg agcccggcgg ggccccggga agcgcccgct ggtacttcca
     2101 catcagcgcc tcccaagcct ggacggccgg gtcggccgcg tcga
//
LOCUS       DAOSIF010000517         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44441_length_2142_cov_3.677048, whole genome shotgun sequence.
ACCESSION   DAOSIF010000517 DAOSIF010000000
VERSION     DAOSIF010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44441_length_2142_cov_3.677048"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLT35_13260"
     CDS             <1..458
                     /locus_tag="PLT35_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30240.1"
                     /translation="AALVDGSGPARVLLGWAAKLDLGNNESATIGVVPQRGEAAGNEE
                     KSMINQEPMVAAEAGERVVTNGEWTSAEKAYAEARRALLDARGDLRMGTEERRRIERT
                     CELARANVEGLCQFAPLEAVGCLSPGHDCAAARKATAARTAAKAKETSA"
     gene            455..868
                     /locus_tag="PLT35_13265"
     CDS             455..868
                     /locus_tag="PLT35_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30241.1"
                     /translation="MKNRIVHLPAIAGLWRQEVTMHPILVHVATLAHGSHLAALAALY
                     AGPYTQDAHDAVSDARNVRDALCDAAAIFEDPGYALKHLDGMAATYAAPVRGVAADIL
                     AARTRLHKAHVEAARMLREAMTAHVRGGGGPHSRF"
     gene            1038..1250
                     /locus_tag="PLT35_13270"
     CDS             1038..1250
                     /locus_tag="PLT35_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30242.1"
                     /translation="MSRYGQFDLAEHSSVTRADVKALRREARAAGDEAMVRTCDAALD
                     RTHDTSQMAAWARCEKAIAEAQAAAS"
     gene            1247..1456
                     /locus_tag="PLT35_13275"
     CDS             1247..1456
                     /locus_tag="PLT35_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30243.1"
                     /translation="MSTATRHVTLSLERALESLLREHKAPALSTLLDEGARAWLRARG
                     VEPGPRQVEPGHVVATRARREKSAK"
     gene            1507..1680
                     /locus_tag="PLT35_13280"
     CDS             1507..1680
                     /locus_tag="PLT35_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30244.1"
                     /translation="MKSDAIKVGQRVLVDGNIVAIVTAIRGSWIEVQEFGTRRRDEYQ
                     ASRVVRAPMSTAR"
     gene            1750..2067
                     /locus_tag="PLT35_13285"
     CDS             1750..2067
                     /locus_tag="PLT35_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30245.1"
                     /translation="MHASDLADLAATSLSRLASDLRAAADPACTSTREAIASTLAQDR
                     ADAVRDLRKLAMALRKGSGDRALATRLASGMVLGHPVSLDALVASNAAAYWRSVRDRL
                     DAA"
BASE COUNT          334 a          771 c          768 g          269 t
ORIGIN      
        1 cggcggcgct cgtggatggc agcggcccgg ctcgcgttct gctcggttgg gccgcgaagc
       61 tcgaccttgg caacaacgag tccgccacga tcggcgtggt cccgcagcgt ggcgaggctg
      121 ccggcaacga ggagaagagc atgatcaacc aggagccgat ggtcgccgct gaggcgggcg
      181 agcgcgtggt cacgaatggc gaatggacca gcgccgagaa ggcgtacgcc gaggcgcggc
      241 gcgcgctgct cgatgcccgc ggcgacctgc gcatggggac ggaggagcgc aggcggatcg
      301 agcgcacgtg cgagctggcg cgcgccaacg tcgaggggct gtgccagttc gcgcccctcg
      361 aagccgtggg ttgcctgagc cccggccacg actgcgccgc ggcgcgtaag gcgacggcgg
      421 cccgcaccgc cgcgaaggcg aaggagacca gcgcgtgaag aaccgcatcg ttcacctgcc
      481 cgcgatcgcg ggcctttggc gtcaggaggt caccatgcac cccattctcg ttcacgtcgc
      541 cacgctcgcc cacggctccc acctcgccgc gctcgcggcc ctctacgccg gcccgtacac
      601 gcaggatgcc cacgacgcgg tctcggacgc gcggaacgtg cgcgacgcgc tctgcgatgc
      661 cgcggccatc ttcgaggacc ccggatacgc cctgaagcac ctcgacggca tggcggcgac
      721 ctacgcggcg cccgtgcgcg gcgtggccgc cgacatcctc gcggcccgca cgcggctcca
      781 caaggcccac gttgaggcag cgcggatgct gcgggaggcg atgaccgccc atgtccgagg
      841 tgggggaggc ccgcattcgc gcttttgagg cgaaggaggt cgagcgcctg cgtgcgctag
      901 gctatcactg gcacctcgac gccaacggac gcgccgcgcg cggcgacgct cccgagaccg
      961 cctgacgcca ccccaccgca cccgccaagc tcgcccggtc cgccgcggcg ggcttgagcc
     1021 gtcaggagga cacgatcatg agcagatacg gccaattcga tctcgccgag cacagcagcg
     1081 tcacccgcgc cgacgtgaag gcgctccgac gtgaggcgcg tgcggcaggc gacgaggcga
     1141 tggtgcggac ctgcgacgcg gcgctcgacc gcacgcacga cacgtcgcag atggccgcgt
     1201 gggcccgttg cgagaaggcg atcgcagagg cgcaggcggc agcctcgtga gcaccgcgac
     1261 ccgtcacgtc accctgagcc tggagcgcgc cctcgaatcg ctcctccgcg agcacaaggc
     1321 gcccgcgctc tcgacgctgc tcgacgaggg cgcccgcgcg tggctccgcg cccgcggcgt
     1381 cgagccgggc ccgcggcagg tagagccggg gcacgtggtt gcaacgaggg cgcggagaga
     1441 gaaatcggca aagtgactgc acgtcacctg ttgactggat acgtatcctg aattagaaag
     1501 ggggtcatga agagcgacgc aatcaaggtc gggcagcggg ttctcgtgga cggcaacatt
     1561 gtggcgatcg tgacggcgat ccgcggctca tggatcgagg tgcaggagtt cgggacgcgc
     1621 cgacgcgacg agtaccaggc gagccgggtg gtccgggcgc cgatgagcac ggcacggtaa
     1681 ggggagccgg ggggctccgg cccgctctgg ggagacccgg ggcgggcctt tcgtgtcagg
     1741 agaaccgcca tgcacgcttc cgaccttgcc gacctcgccg ccacctcgct ctcgcgcctc
     1801 gcctccgacc tgcgcgccgc cgctgaccct gcctgcacct cgacccgcga ggcgatcgcc
     1861 tcgacgctcg cacaggaccg cgccgatgcg gtgcgcgacc tgcgcaagct cgcgatggcc
     1921 ctccgcaagg ggagcggcga tcgcgctctg gccacgcgcc tcgcctccgg tatggttctc
     1981 ggtcaccccg tctcgctcga cgcgctcgtc gcgagcaacg ccgccgccta ctggcgcagc
     2041 gtccgcgacc ggctcgacgc cgcctgaccc acgctgccgc agcgccagcc gaccatcctc
     2101 cggggtggcc ggctcgaccg cgtaggaggt actggatcat ga
//
LOCUS       DAOSIF010000518         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44468_length_2141_cov_4.005273, whole genome shotgun sequence.
ACCESSION   DAOSIF010000518 DAOSIF010000000
VERSION     DAOSIF010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44468_length_2141_cov_4.005273"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLT35_13290"
     CDS             <1..787
                     /locus_tag="PLT35_13290"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HOG30246.1"
                     /translation="AAAPGRTLALAVSDVVGDDLSVIASGPTVGDPTRYQDAVDVIMR
                     FGGPAAVPASVWRALASGAAGLRPDTPKPGSPALARSCTRVIGSQAEALDAACREGLA
                     RGYTVHRHGQPVVGEARDAASEWAARIAAVAAGGGGPAALLSSGETTVTVTGGGRGGR
                     NQELALALARPLAGLACPAVAVSLGTDGVDGPTDAAGAIVDTTTIARASAAGLAAPDA
                     YLNENDSYAFFDALGDLVRTGPTGTNVGDIQIALLLSGGPRCD"
     gene            778..1182
                     /locus_tag="PLT35_13295"
     CDS             778..1182
                     /locus_tag="PLT35_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30247.1"
                     /translation="MRLTLKAFAVLTAALLLALLGGWMWGSAGASRSREALANAGAGL
                     HLANARARLLEARVDLFEVNFGRASLNLEDARAELTAAAQALDRQGRRQDADAIRAAL
                     AVTEEAQRLARRLDQTANARAAEAIGLLPKAP"
     gene            1179..>2141
                     /locus_tag="PLT35_13300"
     CDS             1179..>2141
                     /locus_tag="PLT35_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOG30248.1"
                     /translation="MTRDELLESLHGVLVRPASAREVLQRLGVPRESRAAVRRLLRTL
                     ALDGTLALVRGRRYALPGASDVIVGVLGMHPDGHGFVAPEGGGPGDIYVSREHVKGAM
                     HGDRVVVRRVRASRGGSVEGRVLRIVGRGHTRVVGEFAASRGARAEVVPWDPRWRQPI
                     AVPPGESLGAAAGDMVEAEITAWPTATRGPIGRVVDVLGPLDAPGVDARVVLRAHGIP
                     DAHAPAAEDEARRMGASVRPEDLEGRTDFRADMVVTIDRDDARDFDDAVSVESAGPGV
                     FRLAVHIADVAHYVREGTALDEGARARGTSVYFPDRAIHMFPEAL"
BASE COUNT          240 a          799 c          832 g          270 t
ORIGIN      
        1 cgcggcggcg ccgggccgga cgctggcgct ggccgtgtcg gatgtcgtcg gcgacgacct
       61 ctccgtcatc gcctcggggc cgaccgtggg cgacccgacg cgctaccagg acgcggtcga
      121 cgtcatcatg cgcttcggcg gaccggccgc ggtgccggcc agcgtctggc gggcgctcgc
      181 ctccggcgcg gccggccttc ggcccgacac acccaagccc ggatcgccgg cgctcgctcg
      241 ctcgtgcacg cgcgtgattg gatcgcaggc cgaagccctc gacgcggcct gccgcgaagg
      301 cctggcgcgc ggctacacgg tgcaccggca cggccagccc gtcgtcggcg aggcgcggga
      361 cgccgcgtcc gaatgggcgg cgcgcatcgc tgccgtcgcg gccgggggcg gcgggcctgc
      421 cgccctgctc tcgagcggcg agaccacggt aaccgtaacg ggaggaggcc gcggggggcg
      481 gaaccaggaa ctcgcgctcg cgctggcgcg gccgcttgcc ggcctcgcct gtcctgccgt
      541 cgccgtcagt ctcggcaccg acggcgtgga cgggccaacc gacgcggccg gcgcaattgt
      601 cgacaccacg acaatcgccc gggcgtcggc cgccggtctc gcggcccccg acgcgtacct
      661 gaacgagaat gactcgtacg cgttcttcga tgcgctcggc gatctcgtcc ggaccggacc
      721 cacgggcacg aacgtcggcg acattcagat cgcgttgctg ctctccgggg gaccccgatg
      781 cgactgacgc tcaaggcctt cgccgtcctg actgcggcgc tgctgctcgc gctcctcggc
      841 ggctggatgt ggggatcggc gggcgcttcg cgctcgcgcg aggccctcgc gaatgccggg
      901 gccggcctgc acctggcgaa cgccagggcc cggctgctcg aggcgcgggt ggatctgttc
      961 gaggtgaact tcggccgcgc ctcgctgaac ctcgaggacg cgcgcgccga gttgacggcg
     1021 gccgcgcagg ccctcgatcg gcaggggcgc cgccaggacg ccgacgcgat ccgggccgcg
     1081 ctcgccgtga cggaggaggc gcagcgcctc gcgagacgcc tcgaccagac cgccaacgcg
     1141 cgcgccgcgg aggccatcgg cctgctgccc aaggcgccgt gacgcgcgac gagctcctcg
     1201 agtcgctcca cggcgtcctc gtgcggcccg cctcggcgcg ggaggtgctg cagaggctcg
     1261 gggtcccccg cgaatcccgg gccgccgtcc gccgcctgct ccgcacgctc gcgctcgacg
     1321 ggacgctcgc cctggtccgc gggcgccgtt acgcgctgcc cggcgcgtcg gacgtgatcg
     1381 tcggcgtgct cggcatgcac cccgacgggc acgggttcgt ggcgccggaa ggcggcgggc
     1441 ccggcgacat ctacgtttcg cgcgagcacg tgaagggggc gatgcacggc gaccgcgtcg
     1501 tggtgcgccg cgtccgcgcc agccgcggcg gcagcgtcga ggggcgcgtg ctgcgcatcg
     1561 tcgggcgcgg gcacacccgc gtggtggggg agttcgcggc gtcgcgcggc gcgcgcgccg
     1621 aggtggtgcc gtgggacccc cggtggaggc agcccatcgc cgtgccgccg ggcgagtcgc
     1681 tgggcgccgc cgccggcgac atggtggagg ccgagatcac ggcgtggccg accgccaccc
     1741 gtggtccgat cggccgcgtc gtcgatgtgc tcggcccgct ggacgcgcca ggggtggacg
     1801 cccgggtggt gctgcgggct catgggattc ccgacgcgca cgccccggcg gcggaggacg
     1861 aggcccggcg gatgggcgcg tcggtgcggc ccgaggacct cgagggccgc accgacttcc
     1921 gcgccgacat ggtcgtcacg atcgaccggg acgacgcgcg cgatttcgat gacgccgtgt
     1981 cggtggaatc cgccgggccc ggcgtcttcc gcctcgccgt gcacatcgcc gacgtcgcgc
     2041 actacgtgcg ggagggcacg gcgctggacg agggcgcgcg cgcgcgcggg acgtcggtct
     2101 acttcccgga ccgcgccatc cacatgttcc ccgaggcgct g
//
LOCUS       DAOSIF010000519         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44534_length_2139_cov_1.960173, whole genome shotgun sequence.
ACCESSION   DAOSIF010000519 DAOSIF010000000
VERSION     DAOSIF010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44534_length_2139_cov_1.960173"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="PLT35_13305"
     CDS             complement(<1..1348)
                     /locus_tag="PLT35_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30249.1"
                     /translation="MAGGADSEVKVKFTTEHDGRGAEEANKDLEKTKQKADEAAGGFS
                     RFGKAVSGFKSVMEKASSTLAGFGVMALVGQIMRLVQAFEDAQKAHEDMVAAIGADNA
                     AAGVNRLKEAYDELKESITRVSQETAAVRENADLLIDAERRLEDAALSRREEDEVAAL
                     DKDDPLRKEKEAEIRARYGQQRAETAGRRADDDMAIAEAALRADINQLHANIAERDAK
                     IADLEEAARQYSGKAAAAQEKSIEARNKRGILGRGRNWLGVPRREIEQYAGYANEYNK
                     QAGSALAGVMQGERDNDADRRAIAAAEERLKAMAAQRKAARAESDNAETAAGRTAAAA
                     SADLASAARQRERDRAGLERARREKADLDARKKQLEADKAAAAWRARETSRQAGQARA
                     ALDSHASSAPSGGNKGRWRGALDSLKKNRDDAQAEAAEAARAAVPAARRRDRAARHR"
     gene            complement(1350..1751)
                     /locus_tag="PLT35_13310"
     CDS             complement(1350..1751)
                     /locus_tag="PLT35_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30250.1"
                     /translation="MTDIELTITPAGGGATVTLARSPGRSGDGSPVGPDGLEFRTEKG
                     VLDREPIGADGIDSEHVGCDRRTLTFAVTRVYASEQQALAGLAALEAGCPAQGKVTLG
                     GEDLIGKATLRGLSARLKGCELSAVYTFEGY"
     gene            complement(1748..>2139)
                     /locus_tag="PLT35_13315"
     CDS             complement(1748..>2139)
                     /locus_tag="PLT35_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30251.1"
                     /translation="DEHAAWVIVRKEGVGLRLRGGTVKSSRPGGKLAIPLRAELAGVN
                     PREQFPERGGAFVLTSKGRGPAGRAYLAYREGRALRLAYVLLSSVTIRADRTVLPPSD
                     QLAAAARRACASYARAAVKQSSGGTAK"
BASE COUNT          206 a          690 c          843 g          400 t
ORIGIN      
        1 cacggtgacg ggcggcgcgg tcacggcgac gggcggctgg gacggcggcg cgggcggctt
       61 cggcggcttc ggcctgggcg tcgtcgcggt ttttcttcag cgagtcgagg gcgccgcgcc
      121 accggccttt gttgccgccg gagggcgcgg atgaggcgtg ggagtcgagc gcggcgcggg
      181 cctgccctgc ctgccgcgag gtctcccgcg cgcgccacgc ggcggcggct ttgtcggcct
      241 cgagctgttt cttgcgggcg tcgaggtcgg ctttctcgcg gcgggcgcgc tcgaggccgg
      301 cacggtcacg ctcgcgctgg cgggcggctg aggcgaggtc ggcggaggcg gcggcggcgg
      361 tgcggccggc ggcggtctcg gcgttgtcgc tctcggcgcg ggcggccttg cgctgcgcgg
      421 ccatggcctt gagccgctct tcggcggcgg cgatggcgcg gcggtcggca tcgttgtcgc
      481 gctcgccctg catgacgcct gcaagcgcgc tgccggcctg tttgttgtac tcattcgcat
      541 agcccgcgta ttgctcgatc tcccgcctcg gcacgccgag ccagttcctg ccgcggccga
      601 ggatgccgcg cttgttccgc gcctcgatgg atttctcctg ggcggcggcg gccttgccgg
      661 agtattggcg ggcggcctct tcgaggtcgg cgatcttggc gtcgcgctcg gcgatgttgg
      721 cgtggagctg gttgatgtcg gcgcgcaggg cggcctcggc gatagccata tcgtcgtcag
      781 cgcggcgtcc ggcggtctcg gcgcgctgct ggccgtagcg cgcgcggatc tcggcctctt
      841 tctctttgcg cagggggtcg tctttgtcga gggcggcgac ctcgtcttct tcgcggcgcg
      901 agagggcggc gtcttcgagg cggcgctcgg cgtcgatcag gaggtcggcg ttttcgcgga
      961 ctgcggcggt ttcttggctg acgcgggtga tgctttcttt gagttcgtca taggcttctt
     1021 tgagccggtt gacgccggcg gcggcgttgt ccgcgccgat ggcggcgacc atgtcttcgt
     1081 gcgctttctg cgcgtcttcg aacgcctgga cgaggcgcat gatctggccg acgagtgcca
     1141 tgacgccgaa gccggccagg gtgctgctgg ccttttccat gacgctcttg aagccggaca
     1201 cggccttgcc gaagcggctg aagccgcccg ccgcctcgtc ggccttctgc ttggtctttt
     1261 cgaggtcctt gttggcctct tcggcgccgc gcccgtcgtg ctcggtggtg aacttgactt
     1321 tgacttcgct gtctgctccg cctgccatgt caatagccct cgaaggtgta gacggctgac
     1381 agctcgcagc ctttgagccg cgcggagagg ccgcgcaggg tggctttgcc gatgaggtct
     1441 tcgccgccga gcgtgacctt gccctgcgcc gggcagcccg cctccagcgc ggcgagcccg
     1501 gcgagcgcct gctgctcgga ggcgtagacg cgcgtcacgg cgaaggtcag cgtgcgccgg
     1561 tcgcagccga cgtgttcgct atcgatgccg tccgcgccga taggctcgcg gtcgagcacg
     1621 cctttttctg tgcggaactc gaggccgtcg gggccgacgg gcgagccgtc gccggagcgg
     1681 ccgggggacc gcgcgagcgt taccgtcgcg ccgccgcctg cgggtgtgat ggtgagttcg
     1741 atgtctgtca tttcgcggtg cctccagatg attgcttgac tgcggcgcgg gcgtatgagg
     1801 cgcaggcgcg gcgggcggcc gcggccagtt ggtcggaggg cggcaggacg gtgcggtctg
     1861 cgcggatcgt gacgctggag agcagcacgt atgcgaggcg cagggcgcgg ccttcgcggt
     1921 acgcgaggta ggcgcggccg gccggcccgc gccctttaga ggtgagcacg aaggcgccgc
     1981 cgcgctcggg gaactgctcg cgcgggttga cgccggccag ctcggcgcgc agggggatgg
     2041 cgagcttgcc gccggggcgc gaggacttga cggtgccgcc gcgcaggcgc aggccgacgc
     2101 cctctttacg gacgatgacc cacgcggcgt gctcgtcgg
//
LOCUS       DAOSIF010000520         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44858_length_2128_cov_2.733237, whole genome shotgun sequence.
ACCESSION   DAOSIF010000520 DAOSIF010000000
VERSION     DAOSIF010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44858_length_2128_cov_2.733237"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PLT35_13320"
     CDS             <1..1096
                     /locus_tag="PLT35_13320"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOG30252.1"
                     /translation="RPMAGTERTFRCDTVLVAVGLDPVDEFVAKAKEFGLPVYAAGDS
                     EEIAEASAAMFTGRIRGLEIARALGRDVGEVPPEWHRTADVLKSRPGATITEDLSIPA
                     SGAFPLFHCAQEIPCNPCTSVCPHQSIRIEGGDIMGLPEFVGDQCDACEKCVAICPGL
                     AVTLVDFRRDGDYPTVTVPYEFTAKSIAKGDVVTVLDTEGAILGNVEVTRVREAKAND
                     RCLMVKVKAPRDIATRIAGIRVQEPWVSEPAGAVEPRLDDDTIVCRCERVSAGEIRAL
                     IRSGLRDLNELKALTRAGMGACGGKTCPSLIKRLFREEGVPESQIADFTKRPLFMEVP
                     LGAFAGMAPDGAPPDERPPRHATCAHEGGL"
     gene            1096..>2128
                     /locus_tag="PLT35_13325"
     CDS             1096..>2128
                     /locus_tag="PLT35_13325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG30253.1"
                     /translation="MSTDTFDVVIVGAGSVGLPTACFLAEQGIKPLVIDQFASAGQGS
                     NKAAIGGIRATHSSPAKIRLCLESLRVFSTWEQSRGDNIEWARGGYAFVAYREHERAA
                     LQNLLKAQQRAGLNIGWLERREMLELVPALCAEGLLGGTYSPEDGSASPMLAAHAFYR
                     RASELGVTFRFQERVTGVIRRRGMVVGVRTDKGGYATETIINAGGAWARAVANLAGLD
                     VPVVPDSHEAGITEPVAPFLKPMVVDIRPADGSANYYFYQHKPGGVVFCITPDPPIVG
                     TDRRETSSYLPMIARRMVGLMPCLANIRVRRTWRGLYPMTPDGSPVVGRSKEIEGYIE
                     AVGMCGQGFM"
BASE COUNT          321 a          739 c          775 g          293 t
ORIGIN      
        1 gaggcccatg gccggcacgg agcggacgtt ccggtgcgac acggtcctcg tggccgtcgg
       61 cctcgacccc gtggacgagt tcgtcgccaa ggcgaaggag ttcgggctgc ccgtctacgc
      121 cgccggcgac tcggaggaga tcgcggaggc ctcggcggcg atgttcaccg gccgcatacg
      181 gggcctcgag atcgcgaggg ccctgggccg cgacgtcggc gaggtgccgc cggagtggca
      241 ccgaacggcg gacgtcctca agtccaggcc gggcgcgacc atcacggagg acctgtcgat
      301 cccggcgagc ggggccttcc cgctcttcca ttgcgcgcag gagatcccgt gtaatccctg
      361 cacctccgtg tgcccgcacc agtcgatccg gatcgagggc ggcgacatca tgggccttcc
      421 ggagttcgtc ggcgaccagt gcgacgcctg cgagaagtgc gtggcgatct gcccggggct
      481 cgccgtcacc ctggtcgact tccggcgcga cggcgactac cccaccgtca ccgtgccgta
      541 cgagttcacg gcgaagtcca tcgccaaggg cgacgtcgtg accgtgctcg acacggaggg
      601 cgcgatcctc gggaacgtgg aggtcacccg cgtccgcgag gcgaaggcca acgaccgctg
      661 cctgatggtg aaggtcaagg ccccgcgcga catcgccacg cgcatcgcgg gcatccgcgt
      721 gcaggagcct tgggtgagcg agccggccgg ggctgtcgag ccgcgcctgg acgacgacac
      781 gatcgtgtgc cggtgcgagc gcgtgagtgc cggcgagatc cgggcgctca tcaggtcggg
      841 cctgcgcgac ctcaatgagc tgaaggccct gacccgcgcc gggatggggg catgcggggg
      901 caagacgtgc cccagcctga tcaagcggct cttccgggag gagggcgtgc cggagagcca
      961 gatcgcggac ttcaccaagc ggcccctgtt catggaggtc ccgctcggcg ccttcgcggg
     1021 catggcgccc gacggcgcgc cgccggacga acggcccccg cggcacgcga catgcgcgca
     1081 cgaagggggc ctgtgatgag cacggacacc ttcgacgtcg tcatcgtcgg cgccgggagc
     1141 gtgggcctgc ccacggcgtg cttcctcgcc gaacagggca tcaagccgct cgtcatcgac
     1201 cagttcgcga gcgccgggca gggcagcaac aaggccgcca tcggcggcat ccgggccacc
     1261 cactcgtcgc ccgccaagat ccgcctgtgc ctcgagtcgc tgcgcgtctt ctccacctgg
     1321 gagcagtccc gcggcgacaa catcgagtgg gcccgcggcg ggtacgcgtt cgtcgcctac
     1381 cgcgagcacg agcgcgcggc gctgcagaac ctgctcaagg cccagcagcg cgcgggcctc
     1441 aacatcggat ggctggaacg ccgggagatg ctcgaactgg tgccggccct ctgcgccgag
     1501 ggcctgctcg gcggcaccta ctcgcccgaa gacggatcgg cgtcacccat gctggcggcg
     1561 cacgcgttct accggcgggc gtccgagctg ggcgtcacgt tccggttcca ggagcgggtc
     1621 accggcgtga tccggagacg cggcatggtg gtcggcgtcc gcaccgacaa gggcggctat
     1681 gcgaccgaga ccatcatcaa cgccggcggc gcctgggcga gggccgtggc caacctggcc
     1741 ggcctcgacg tgccggtcgt gcccgacagc cacgaggccg gaatcaccga gcccgtggcg
     1801 ccgttcctca agcccatggt ggtggacatc cgccctgccg acggatcggc caactactac
     1861 ttctatcagc acaagccggg cggggtggtc ttctgcatca cgccggaccc gccaatcgtc
     1921 ggcacggacc gccgcgagac gtcgtcgtac ctgccgatga tcgcgcggcg gatggtgggg
     1981 ctgatgccgt gcctggcgaa catcagggtc cggcggacgt ggcgcggcct ctacccgatg
     2041 acgccggacg ggtcgccggt ggtcgggcgg tcgaaggaga tcgaggggta catcgaggcc
     2101 gtcggcatgt gcggccaggg cttcatgc
//
LOCUS       DAOSIF010000521         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44946_length_2125_cov_3.634300, whole genome shotgun sequence.
ACCESSION   DAOSIF010000521 DAOSIF010000000
VERSION     DAOSIF010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44946_length_2125_cov_3.634300"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLT35_13330"
     CDS             complement(<1..552)
                     /locus_tag="PLT35_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG30254.1"
                     /translation="MLIQAVFFDVDFTLIYPGPSLQAEGYGRFCARRGVAVDTSRFRE
                     AVRRAATILDDVRAHDDDARLFVDYARAIIGHMGGGGPGAELAARDIYDEWAVFRHFF
                     LYDDVQPALRALSAAGLRIGLISNTRRPLDEFAAHFDLRGLFAASVSSAQLGFLKPHP
                     AIFQAALAAVGVPAAAAVMVGDSV"
     gene            683..1504
                     /locus_tag="PLT35_13335"
     CDS             683..1504
                     /locus_tag="PLT35_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30255.1"
                     /translation="MTTRIAAVLVLAALAAPSTAVAGPRVTITFRSGDRIRGELLDFG
                     GVGFTIRVGNNEQRIPPGDVAVIDLTNGGVTAAEAAKIRDGRAFVVLHGGDTFYGSLY
                     DIGGTDPLQIIFRTPYGDRTVTSSQVARIYFARWIGMPTVEASGGTTQEGSVDQGGGV
                     VVPGNVCWTATTYTVRRGQHVAFDARGEIRLSSDPNDVAGVGGSKIGRMLSNAPLPNV
                     LAGALIGRVGYSRPFGIGDQQQGLQMPADGVLFLGINDADCHDNAGHFRVRITPM"
     gene            1616..>2125
                     /locus_tag="PLT35_13340"
     CDS             1616..>2125
                     /locus_tag="PLT35_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG30256.1"
                     /translation="MADWKSTLNLPTTSFPMKANLQVAEPQMLARWDAIGLYRKLRDR
                     RRGAPVFYLHDGPPYANGNVHLGTALNKILKDVVVRSRSMAGFDAPYVPGWDCHGLPI
                     ELKLLKELGPKAREMSVAGLRRACREYAGRYVDIQREQFKRLGVLGDWGDPYLTMDYR
                     YQAAIVRALG"
BASE COUNT          327 a          748 c          749 g          301 t
ORIGIN      
        1 cacgctgtcg cccaccatca cggcggcggc ggcgggcacg ccgacggcgg cgagggcggc
       61 ctggaagatc gccgggtgcg gtttgaggaa gccgagctgc gccgacgaca ccgacgccgc
      121 gaagaggccg cgcaggtcga agtgcgccgc gaactcgtcg agcggccgcc gggtgttcga
      181 gatgaggccg attctcaggc ccgcggccga aagcgcccgc agcgccggct gcacgtcgtc
      241 gtacagaaag aagtgccgga agaccgccca ttcgtcgtag atgtcccggg ccgccagctc
      301 cgcgccaggc ccgccgccgc ccatgtgccc gatgatggcg cgcgcgtagt ccacgaagag
      361 ccgcgcgtcg tcgtcgtggg ccctgacgtc gtcgaggatc gtcgccgccc gccgcacggc
      421 ctcgcggaac ctggaggtgt ccacggccac gccgcggcgg gcgcagaagc ggccgtaccc
      481 ttcggcctgg agcgacgggc ccgggtagat cagcgtgaag tccacgtcga agaacaccgc
      541 ttggatcagc accgccgagc ctcccgccgc cgtccgggcg ccgctcatta taccggcgcg
      601 cgcgccgggg cccggcggcc gttgcgtata atggcttgtg ccgacggcgc cgcgcgccgc
      661 gacggcacga aggagacgac cgatgaccac acgcatcgcg gcggttcttg tcctggcggc
      721 gcttgccgcg ccgtcaacgg cggtcgctgg cccgcgcgtc acgatcacgt tccgctcggg
      781 ggatcgcatc cgtggcgagc tcctcgactt cggcggcgtc gggttcacga ttcgggtagg
      841 caacaacgaa cagcggattc ctcccggcga cgtcgccgtc atcgacctga ccaacggcgg
      901 cgtgaccgcg gcggaagcgg cgaagatccg ggacgggcgc gccttcgtcg tcctccacgg
      961 gggcgacact ttctacggca gcttgtacga cattggcggg accgaccccc tccagatcat
     1021 ctttcggacg ccctacggcg accggacggt gacctcgtcc caggtggcgc gcatctactt
     1081 cgcgaggtgg atcgggatgc cgacggtcga agcttctggc ggcacgaccc aggaggggag
     1141 cgtcgaccag ggcggcggcg tcgtcgtccc cggaaacgtc tgttggaccg ccacgacgta
     1201 cacggtccgc cgcgggcagc acgtcgcctt cgatgcccgg ggcgagatcc ggttgagctc
     1261 ggatccgaac gacgtcgcgg gtgtcggcgg gtcgaagatc ggccggatgt tgtcgaacgc
     1321 gccgctcccg aacgtcctcg ccggcgcgtt gatcggacgc gtcggctact cgcgcccgtt
     1381 cggcatcggc gatcagcagc agggcctgca gatgccggcg gacggcgtgc tgttcctcgg
     1441 catcaacgac gccgactgcc acgacaacgc cggccatttc agggtcagga tcacgccgat
     1501 gtagcggcgg ctggaccgtg gcgtcgattg tcgcacccgg gcggcccggg gatccgccga
     1561 gttcgggcgg gtcccgggcc gctcgcgcgt ttggcgctaa aatgtaacga tcaccatggc
     1621 cgactggaag agcaccctca acctgcccac gacaagcttc ccgatgaagg ccaacctgca
     1681 ggtggccgag ccgcagatgc tggcccgctg ggacgccatc ggcctctacc gcaagctgcg
     1741 cgatcgccgc cggggcgcgc cggtcttcta cctccacgac ggcccgccct atgcgaacgg
     1801 caacgtgcac ctcggcaccg cgctgaacaa gatcctcaag gacgtcgtgg tgcggtcgcg
     1861 gtccatggcg ggattcgacg ccccgtacgt tcccggctgg gactgccacg gcctgcccat
     1921 cgagctcaaa ctgctcaagg agctcgggcc gaaggcacgc gagatgagcg tggccggcct
     1981 gcgccgggcc tgccgggagt acgcgggccg ctacgtggac atccagcgcg agcagttcaa
     2041 gcgcctcggt gtgctcggcg actggggcga tccctacctg acgatggact accgctacca
     2101 ggccgccatc gtgcgggcgc tcggc
//
LOCUS       DAOSIF010000522         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_44954_length_2125_cov_3.112560, whole genome shotgun sequence.
ACCESSION   DAOSIF010000522 DAOSIF010000000
VERSION     DAOSIF010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44954_length_2125_cov_3.112560"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLT35_13345"
     CDS             <1..787
                     /locus_tag="PLT35_13345"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOG30257.1"
                     /translation="TPLTFANLGVKPGTDFANIDLSGTTVSLGAHAGLLYKASERLTL
                     GARVMSSQTPKVDDGKIKTEQIATGYVLPIPLGPTLPAGVPIDAIVKPQFGPGGKLSN
                     QSASTSLPLPLQAVAGLAYRASPKVLLLADYQYVRWSAFDKLVIDGEYLDSTLEEAYR
                     DTHGIRLGTEIALDEQTVARAGFIVHGAAAPDQTVTPLLPEGKRWLLALGLGRQLTPH
                     LRLDAAYTYLAQPERAGRSSDGPNNGVYNFMAHLFGVTFSFGF"
     gene            1145..1981
                     /locus_tag="PLT35_13350"
     CDS             1145..1981
                     /locus_tag="PLT35_13350"
                     /inference="COORDINATES: protein motif:HMM:NF006132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG30258.1"
                     /translation="MNLETLTRMYDFTGRTVAVTGGTGVIGGALVRGLAGCGASVAVI
                     DRNTAPGPALLESLGPAAAARVEVIGADVLDPDSIGRALDAILARFGSVDALINGAGG
                     NHPKATSSADVPFFDLPPDAIRWVFDLNVLGTILPSQAFGRVMAKAGAGVILNVSSMN
                     ALRPLTRIAAYSAAKAGVSNFTQWLAVHLAQEYSPALRVNAIAPGFLLTDQNRFLLTD
                     QETGALTPRGQSILAHTPMGRFGVTDDLLGAALWLLSPASAFVTGIVLPVDGGFSAFS
                     GV"
BASE COUNT          290 a          789 c          754 g          292 t
ORIGIN      
        1 aacgccgctg accttcgcca acctcggcgt gaagcccggc accgatttcg cgaacatcga
       61 cctgtcggga acgacagtca gcctgggcgc ccacgccggc ctgctctaca aggccagcga
      121 gcgcctcacg ctcggcgcgc gcgtcatgag cagccagacg cccaaggtgg acgacgggaa
      181 gatcaagacc gagcagattg ccaccggcta cgtgttgccg atcccgctgg gcccgacgct
      241 gccggccggc gtgcccatcg acgcgatcgt gaagccgcag ttcggcccgg gcggcaagct
      301 gagcaaccag tcggccagca cgagcctgcc gctcccgctg caggccgtcg ccggcctggc
      361 ctaccgggcc agtccgaagg tcctgctcct cgccgactac cagtacgtgc ggtggagcgc
      421 gttcgacaag ctggtgatcg acggcgagta cctggattcc acgctggaag aggcgtacag
      481 ggacacgcac ggcatccgcc tgggcacgga gatcgcgctc gacgagcaga ccgtggcgcg
      541 cgccggcttc atcgtccatg gcgcggcggc tcctgaccag acggtgacgc cgctgctccc
      601 cgagggcaag cgatggctgc tggccctggg gctcggccgt cagctgaccc cgcacctgcg
      661 cctcgacgcg gcctacactt atctggcgca gccggaacga gccggccgca gcagcgacgg
      721 cccgaacaac ggcgtctaca acttcatggc gcacctgttc ggcgtcacct tctcgttcgg
      781 cttctaggcc gcaccgcccg ccgcgtgcgt ccctcggccg ggtgcgccgc gcgccgcccg
      841 gccgcgggac cggccgcggg gcgggccgcc tcgccgggcc ctccacggcc ggcccatgga
      901 gaggcggctg tccctcggcg gccgcgtcag ccgtgaccgc gcagcggccg ggtgcgccgc
      961 gcgaccggac tggagggtcc tgcgacggcc cgggccgcct tgacttccct gttcggggac
     1021 gcgtatcgtc tcgcaagtgg acgtgcctcc cgaggggcgc tcgcgtcccg tcggcaagac
     1081 caccgcgcgc cccgccctcg acggccaggc cccacgacgg tgcggacgct gccggagcac
     1141 ccccatgaac ctcgagacgc tcacgcggat gtacgacttc accggcagga ccgtggcggt
     1201 caccggcggc accggcgtca tcggcggggc gctggtccga gggctcgccg gctgcggggc
     1261 cagcgtcgca gtcatcgacc gcaacacggc gccgggcccg gcgctcctcg agtcgctggg
     1321 gccggccgcg gccgcccgcg tcgaggtgat cggcgccgac gtgctggacc ctgacagcat
     1381 cggccgggcg ctcgacgcga tcctggcccg cttcggatcg gtggacgccc tcatcaacgg
     1441 cgccggcggc aaccacccga aggcgaccag cagcgcggac gtgccctttt tcgatctccc
     1501 cccggacgcc atccgctggg tcttcgacct gaacgtcctc ggcaccatcc tgccgtcgca
     1561 ggcgttcggc cgggtgatgg cgaaggcggg cgcgggcgtc atcctgaacg tctcgtcgat
     1621 gaacgcgctc cgcccgctga cgaggatcgc cgcctactcg gccgcgaagg cgggggtcag
     1681 caacttcacg cagtggctcg ccgtgcacct cgcgcaggag tactcgccgg ccctgcgcgt
     1741 gaacgccatc gcgccgggct tcctgctcac ggaccagaac cgcttcctgc tgaccgacca
     1801 ggagaccggc gcgctgaccc cgcgcgggca gtcgatcctg gcgcatacgc ccatgggccg
     1861 gttcggtgtg accgacgatc tgctcggggc ggcgctgtgg ctgctctcgc cggcctcggc
     1921 gttcgtgacc ggtatcgtgc tgccggtcga cggcgggttc tcggcgttca gcggggtctg
     1981 acaagacggg cggcgaccgg gaaaggcggc ggcagacgtg gtcatcggag cggggtcgtg
     2041 cgatcggcat ctcccggacg aggaggtgcg ccgtctcgtg gtcgacggcc tggccgggct
     2101 gccgctcgac ggaaggcggg tcctg
//
LOCUS       DAOSIF010000523         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_45086_length_2120_cov_4.045521, whole genome shotgun sequence.
ACCESSION   DAOSIF010000523 DAOSIF010000000
VERSION     DAOSIF010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45086_length_2120_cov_4.045521"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLT35_13355"
     CDS             complement(<1..609)
                     /locus_tag="PLT35_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG30259.1"
                     /translation="MDLIWLIPLLPGLGALVNGLVGVRAFGKRTAGLVANAAMAAALA
                     LALYAFWQLLSLPADAREHTVRLAAWIPPIPLALGNGAIGGFTVPWGFRLDPLSGLMI
                     LVVTLVGFLIHVYSTGYMHGETRGAYARFFAYLNLFAFFMLVLVLGDNFLVMFVGWEG
                     VGLCSYLLIGYYYEKQSASDAGKKAFIVNRIGDWGFIVGIFMV"
     gene            complement(615..917)
                     /gene="nuoK"
                     /locus_tag="PLT35_13360"
     CDS             complement(615..917)
                     /gene="nuoK"
                     /locus_tag="PLT35_13360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOG30260.1"
                     /translation="MITATHYLLLSVGLFSLGVIGVLTRRNIIIILMSIELMLNAVNI
                     NLVAFSHELQNVVGQAFAVFVIVIAAAEAAVGLGIILAFYRNKETVNIDEMNLLRW"
     gene            complement(922..1422)
                     /locus_tag="PLT35_13365"
     CDS             complement(922..1422)
                     /locus_tag="PLT35_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOG30261.1"
                     /translation="MAEAAAFYILAALILGFGVLVVTAKSAVHSVLFLVADFLCVAAL
                     YVTLQAEFLAVIQVMVYAGGIVVLYLFVVMLVNLKRPPDAQRDPRRLGRLGAVLAVAV
                     MVELAAILAVGWSRPEGEAGALGPSNVAQVGRALYTDYLIPFELASILLLVAMIGAIL
                     LARREM"
     gene            complement(1449..>2120)
                     /locus_tag="PLT35_13370"
     CDS             complement(1449..>2120)
                     /locus_tag="PLT35_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOG30262.1"
                     /translation="VGLLYVVSVGTLGIYGIILGGWASNSKYPLLGSLRSSAQIISYE
                     IAVTMTLVTVIVLSGTLSMTGIVHAQLDKGWWFGFMQPVAFFIYFVGGLAETNRLPFD
                     LPEAEQELVAGFHTEYSGMRFAFFFLAEYTNMIVISAVATTLFLGGWLPPFPNVAWLG
                     FLGVVPGWAWFMLKTFGFLYVFLWVRATFPRYRYDQLMRLGWKWLIPLSIANLVVTGL
                     MKVLV"
BASE COUNT          431 a          712 c          701 g          276 t
ORIGIN      
        1 caccatgaag atcccgacga tgaagcccca gtcgccgatc cggttgacga tgaacgcctt
       61 cttgcccgcg tccgatgcgc tctgcttctc gtagtagtac ccgatgagga ggtacgaaca
      121 gagcccgacg ccctcccagc cgacgaacat cacgaggaag ttgtcgccca ggacgagcac
      181 gagcatgaag aaggcgaaca ggttcaggta ggcgaagaac cgcgcgtagg cgccccgcgt
      241 ctcgccgtgc atgtagccgg tcgagtagac gtggatcagg aacccgacca gcgtgacgac
      301 gagaatcatc agtcccgaca gcgggtcgag ccggaagccc cacggcaccg tgaagccgcc
      361 gatcgccccg tttcccaggg cgagggggat cggcgggatc cacgctgcca gccgcacggt
      421 gtgctcgcgg gcgtcggcgg gcagcgacag gagctgccag aaggcgtaca gcgccagggc
      481 cagcgccgcg gccatcgccg cgttggcgac gagccccgcc gtgcgtttgc cgaaagcgcg
      541 cacgccgacg aggccgttga cgagggcgcc caggccgggc agcagcggaa tcaaccagat
      601 caggtccata cggctcacca ccgcagcagg ttcatctcgt cgatgttgac ggtttccttg
      661 ttgcggtaga acgcgaggat gatgccgagg ccgacggcgg cctcggcggc ggcgatgacg
      721 atgacgaaca ccgcgaacgc ctggccgacg acgttctgga gctcgtgcga gaacgccacg
      781 aggttgatgt tcaccgcgtt gagcatcagc tcgatcgaca tcaggatgat gatgatgttc
      841 cggcgcgtca ggacgccgat gacgcccagc gagaacaggc cgaccgagag cagcaggtag
      901 tgggtggcgg taatcatcgg cctacatctc cctcctcgcc aggaggatcg cgccgatcat
      961 cgcgacgagc agcaggatcg acgccagctc gaaggggatc aggtagtcgg tgtagagcgc
     1021 gcggccgacc tgggccacgt tgctcgggcc cagcgcgccc gcctcgccct ccggccgcga
     1081 ccagccgacc gccaggatcg cggccagctc gaccatgacg gcgaccgcca gcacggcacc
     1141 cagccgtccc agccggcgcg gatcgcgctg cgcgtccggg gggcgcttca ggttcaccag
     1201 catgaccacg aagaggtaca gcacgacaat gccgccggcg tacaccatga cctggatgac
     1261 ggcgaggaac tccgcctgca gcgtcacgta gagcgcggcc acgcacagga agtcggcgac
     1321 caggaacagg acgctgtgca cggcgctctt cgccgtgacg accagcacgc cgaaccccag
     1381 gatgagcgcc gcgaggatgt agaaggcagc tgcttcagcc atggctctcc gctacgggcg
     1441 tcgccccctc acaccagcac cttcatcagc ccggtcacca ccaggttggc gatggacagc
     1501 gggatcagcc acttccagcc caggcgcatc agctggtcgt agcggtagcg cgggaaggtc
     1561 gcccggaccc acagaaagac gtacaggaac ccgaaggtct tgagcatgaa ccacgcccac
     1621 ccgggaacga cgccgaggaa ccccagccac gccacgttcg ggaacggcgg cagccagccc
     1681 ccgaggaaga gcgtcgtcgc caccgccgag atgacgatca tgttcgtgta ctcggcgagg
     1741 aagaagaacg cgaaccgcat ccccgagtac tcggtgtgga agcccgccac cagctcctgc
     1801 tcggcctccg ggaggtcgaa cggcaggcgg ttcgtctcgg ccagcccgcc cacgaagtag
     1861 atgaagaacg cgaccggctg catgaagccg aaccaccacc ccttgtcgag ctgggcgtgg
     1921 acgatgccgg tcatgctgag cgtgcccgac agcacgatga ccgtgaccag cgtcatcgtg
     1981 acggcgatct cgtagctgat gatctgcgcc gacgagcgca ggctgcccag gagcgggtac
     2041 ttgctgttcg aggcccagcc gccgaggatg atcccgtaga tgcccagcgt cccgaccgag
     2101 acgacgtaga gcaggcccac
//
LOCUS       DAOSIF010000524         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_45152_length_2118_cov_3.091614, whole genome shotgun sequence.
ACCESSION   DAOSIF010000524 DAOSIF010000000
VERSION     DAOSIF010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45152_length_2118_cov_3.091614"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLT35_13375"
     CDS             <1..997
                     /locus_tag="PLT35_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG30263.1"
                     /translation="ILQEVVNVLPSACQYPELACASITLKSRCYRTAGVVEPRRLQRA
                     DILVDRQPVGVLELGYAERLGGGPAPEFLEEEQTLLETVAGRLSEVIALKEAQSQLAT
                     YQEHLRSMAAELAMAEERERRSLAISLHDRIGQSLAVAKLKLETLKHVLPPQYHTRLD
                     DITALIKDIVNDTRSLTIDISPPILYELGLEPAVSWLGEHITRQSGLPVDVYGSGEGI
                     ELNEGVRVVVFRSIQELLINAVKHSGASRVSVRISRHGADDVRVVVEDNGAGFVLPER
                     GWHPSAASGFGLFSLRERIGHLGGTVEIESGPGRGTKVVLTVPGRPRSPGEEARG"
     gene            994..1701
                     /locus_tag="PLT35_13380"
     CDS             994..1701
                     /locus_tag="PLT35_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG30264.1"
                     /translation="MRILLADNHRLFCEGLRTLFATRPGMEIVGEANDGRLAVRLARE
                     LSPDVVIMDIGMPELNGIEATRQIRAELPHVKVLAVSMHSDRQYVAGMLAAGACGYVL
                     KDSAFSELADAIRAVARGGRYLSPEIVGVVLDDYVHRLSPSPDSAAARLSDREREVLQ
                     LMAEGHATADIAARLHISRKTVETHRKSIMLKLELHSVADLTKFAVREGITSLDVGRS
                     GRPPAVTAGPAAPRAKP"
BASE COUNT          334 a          725 c          757 g          302 t
ORIGIN      
        1 catcctccag gaagtggtga acgtcctgcc gtcggcgtgc cagtatccgg agctggcgtg
       61 cgcctcgatc acgctgaaga gccgctgcta ccgcacggcc ggcgtcgtgg agccccggcg
      121 cctccagcgg gccgacatcc tcgtggatcg ccagccggtc ggcgtgctcg aactgggata
      181 cgccgagcgg ctcggcggcg ggccggcccc ggagttcctc gaggaggagc agacgctgct
      241 ggagacggtc gccggacggc tctccgaggt catcgccctg aaggaggcgc agagccagct
      301 cgccacctat caggaacacc tgcgttcgat ggccgccgag ctggcgatgg cggaagaacg
      361 ggagcggcgg agcctggcca tcagcctgca cgatcggatc gggcagagcc tggcggtcgc
      421 caagctcaag ctcgagacgc tcaaacacgt gcttccgccg cagtaccata ccaggctgga
      481 cgacatcacg gcgctcatca aggacatcgt gaacgacacc cgctcgctga cgatcgacat
      541 cagccccccg atcctgtacg aacttggcct cgaaccggcc gtttcgtggc tgggggagca
      601 tatcacccgc cagtccggcc ttccggtgga cgtctacggc agcggcgagg gcatcgagct
      661 gaacgagggc gtcagggtgg tcgtgttccg gtcaatccag gaactgctca tcaacgccgt
      721 gaaacattcc ggcgcgtcgc gcgtctcggt ccggatctcg cggcacggcg ccgacgacgt
      781 gcgcgtggtc gtggaggaca acggcgcggg gttcgtgctg ccggagagag gctggcaccc
      841 ctcggccgca agcgggttcg gcctcttcag cctccgcgag cgcatcggcc acctcggcgg
      901 cacggtcgag atcgaatcag gcccggggcg gggaaccaag gtcgtgctga cggtccccgg
      961 ccggcccagg tcgcccggcg aggaggcgcg gggatgagga tcctgcttgc cgacaaccac
     1021 cggctgttct gcgaggggtt gcggacgctc ttcgcgaccc gccccggcat ggagatcgtc
     1081 ggggaggcca acgacggccg cctcgcggtg cgcctcgcgc gcgaactgtc gcccgatgtc
     1141 gtcatcatgg acatcgggat gccggagctg aacgggatcg aggccacgcg gcagatccgg
     1201 gccgaactcc cgcacgtgaa ggtgctcgcg gtgtcgatgc actcggaccg gcagtatgtg
     1261 gcgggcatgc tggccgccgg ggcgtgcggg tacgtcctca aggacagcgc gttcagcgag
     1321 ctcgccgacg cgatccgcgc cgtcgctcga ggcgggcgct acttgagccc ggagatcgtc
     1381 ggcgtggtgc tggacgacta cgtgcaccgc ctcagcccgt cgcccgactc ggccgccgcc
     1441 aggctgtcgg accgggagcg cgaggtgctg cagctgatgg ccgaggggca cgcgacggcg
     1501 gatatcgcgg cccggctgca catcagccgc aagacggtcg agacccatcg gaagagcatc
     1561 atgctcaaac tcgagctgca cagcgtcgcg gacctgacga agttcgccgt gcgcgaggga
     1621 atcacctccc tggatgtcgg gcgtagcggg cggccgccgg ccgtgacggc cggccccgcc
     1681 gcccctaggg cgaagcccta gcctcgtacg tcaccctttc ggcgtttgtg gcccggcgca
     1741 ggccgtccgg aggcgcgccg gccgccgctg gcgccggctt ccgcgggctg tcgccatggc
     1801 cggcgcgccg tggggctgac cggcgccgct cagccggcag ggagggccga aatcaggcga
     1861 ctggacgcgc ggaatccggc gccgcccgat gcccgcctgg cgccagccgc ctagaatcgc
     1921 aggacgggcg tgttcgacgt ccgcacccag aggatgcacg ccatgaacac gaatccagtt
     1981 gcagcgaagg ttcgctcgag cgactcgcag tcgacgacgc tgctcatccg gacgcatggc
     2041 ctggccatcg aagctgaatt cgccgacctc gtgccctgcg agttcccgat ctggacgagc
     2101 gcgtcagttc ccgtcgag
//
LOCUS       DAOSIF010000525         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_45333_length_2112_cov_2.609626, whole genome shotgun sequence.
ACCESSION   DAOSIF010000525 DAOSIF010000000
VERSION     DAOSIF010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45333_length_2112_cov_2.609626"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /gene="pgsA"
                     /locus_tag="PLT35_13385"
     CDS             complement(<1..481)
                     /gene="pgsA"
                     /locus_tag="PLT35_13385"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOG30265.1"
                     /translation="MNLPNALTLTRIFLVPLLVVVLLTKVEWPVMGVSSQVLGAAIFA
                     VAAVTDWLDGYLARRRRQVTTLGQLMDPIADKLLTAAAFISLVQMGAAPAWMVAIIIG
                     REFAVTGLRSVAYARGVVIPASPLGKAKMISQIAAILLLILGHEGFRPFFLLGKIALW
                     "
     gene            527..1597
                     /locus_tag="PLT35_13390"
     CDS             527..1597
                     /locus_tag="PLT35_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOG30266.1"
                     /translation="MKAILLAGGKGTRLRPLTLHTPKPIVPVFDRAFLNYQIDLVRTV
                     PEIDEIVLSLNYQPRRIQEVLGEGGDGGPRISYVVEPSPLGTGGAIKYATGADTGTVV
                     VFNGDVMTQVNLGDVVRLHRERRAKATIVLTPVDNPSEYGLVETDADGNVRRFLEKPG
                     PNDVTCDTINAGIYVLEPETFDRIPKDTASSIERAFFPSLVERQEAFVAFVYHGYWID
                     IGTPEKYLQVHRDIMDRRFLAEPFAAQPRGAQVLGRDVRIEDGASVEGPCYVGDRTVL
                     KAGARVLPYSVVGRDCILEEGAVIDASIVWPNGRVSRDAALRNAIVGRHCHIGRDVVV
                     EAGTILGDKSSVTDHSRLGAGL"
     gene            1611..>2112
                     /locus_tag="PLT35_13395"
     CDS             1611..>2112
                     /locus_tag="PLT35_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30267.1"
                     /translation="MRVNPGIFKAYDIRGIYPADVDEAAARQIGRAFVAYLGARRIAV
                     GRDMRISAPSIAAAFIEGAREQGADVTDYGMIATDMLYFAVARDHHDGGAMITASHNP
                     KDYIGMKLVRAEAFPLSGEAGISQIRDMITGGAIPAPAPAPGSLSVNAALLDDYVAHV
                     LGFIDAA"
BASE COUNT          351 a          719 c          731 g          311 t
ORIGIN      
        1 cccacagcgc gatcttgccc agcaggaaga acggcctgaa gccctcgtgg cccaggatga
       61 gcaggaggat cgcggcgatc tggctgatca tcttggcctt gcccagcggg gacgccggga
      121 tgacgacgcc gcgggcgtag gcgacgctgc gcaggcctgt gacggcgaac tcgcggccga
      181 tgatgatggc caccatccac gccggggcgg cgcccatctg gaccagagaa atgaacgcgg
      241 cggcggtgag cagcttgtcc gcgatcggat ccatgagctg tccgagggtg gtgacctgcc
      301 tgcggcgccg ggcgaggtag ccatccagcc agtccgtgac ggcggcgacg gcgaagatcg
      361 ccgcgccgag cacctggctc gacacgccca tgaccggcca ctcgaccttc gtcagcagga
      421 cgacgaccag cagcggcacc aggaagatcc gggtgagggt cagcgcgttc gggaggttca
      481 tccggggcct attttagcgt gcttgcgata agatggtgac gttgtcatga aagccattct
      541 cctcgccggg ggcaagggca cgcggctgcg tcccctcacg ctccacacgc cgaaaccgat
      601 cgtgcccgtc ttcgatcggg ccttcctcaa ctaccagatc gacctcgtgc ggacggtgcc
      661 cgagatcgac gagatcgtgc tgagcctcaa ctaccagccc cggcgcatcc aggaggtgct
      721 cggcgagggc ggcgacggcg ggccgcgcat ctcgtacgtc gtcgagccga gcccgctcgg
      781 cacgggcggc gcgatcaagt acgcgacggg cgccgacacc ggcacggtcg tggtgttcaa
      841 cggcgacgtg atgacgcagg tcaacctcgg cgacgtcgtg cgcctccacc gggagcggcg
      901 cgcgaaggcc accatcgtgc tcacgccggt ggacaatccg tccgagtacg gcctcgtcga
      961 gacggatgcg gacggaaacg tgcggcggtt tctcgagaag ccgggcccga acgacgtcac
     1021 ctgtgacacg atcaacgccg gcatctacgt cctggagccg gagacgttcg accgcattcc
     1081 gaaggacacc gcgtcgtcga tcgagcgggc cttcttcccg tcgctcgtgg agcggcagga
     1141 ggcgttcgtg gcgttcgtgt accacggcta ctggattgac atcggtacac ccgagaagta
     1201 cctgcaggtg caccgcgaca tcatggaccg ccgattcctg gccgagccgt tcgccgcgca
     1261 gccgcgcggc gcgcaggtcc tcggccgcga cgtccgcatc gaagacggcg catcggtgga
     1321 agggccgtgt tacgtgggcg acaggaccgt cctcaaggcc ggtgcgcgcg tgcttcccta
     1381 cagcgtggtc ggtcgggact gcatcctgga ggaaggggcc gtgatcgacg catcgatcgt
     1441 ctggcccaac gggcgggtct cccgcgacgc ggcgctccgg aacgcgatcg tgggccgcca
     1501 ctgccacatc ggtcgggacg tcgtcgtcga ggccggcacg atccttggcg acaagtcgtc
     1561 ggtgacggat cattcccgcc tgggcgcggg cctgtgaggg agaggcatcg atgcgcgtga
     1621 accccggcat tttcaaggcg tacgacatca gaggcattta ccccgccgac gtggacgagg
     1681 cggcagcccg gcagatcggc cgcgccttcg tggcctacct cggcgccagg cgcatcgccg
     1741 tcggccggga catgcgcatc tcggcgccct ccattgccgc cgccttcatc gagggcgccc
     1801 gcgagcaggg cgccgacgtg accgactacg ggatgatcgc caccgacatg ctgtacttcg
     1861 cggtggccag ggaccaccac gacggcggcg cgatgatcac ggcgtcgcac aacccgaagg
     1921 actacatcgg gatgaagctc gtccgcgccg aggcgttccc gctgagcggc gaggcgggca
     1981 tatcccagat ccgggacatg atcaccggcg gcgccatccc cgcgcccgcg ccggcgccgg
     2041 gctcgctctc ggtgaacgcc gccctcctcg acgactacgt ggcgcacgtc ctcggcttca
     2101 tcgacgccgc cg
//
LOCUS       DAOSIF010000526         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_45399_length_2110_cov_3.184915, whole genome shotgun sequence.
ACCESSION   DAOSIF010000526 DAOSIF010000000
VERSION     DAOSIF010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45399_length_2110_cov_3.184915"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLT35_13400"
     CDS             <1..854
                     /locus_tag="PLT35_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30268.1"
                     /translation="GLGEHFGIERHGNAPIDPHGEFAGRNLLHVAIPAEALAARHRLP
                     LGQVRRLLAGAKAAMLAARAARPRPLRDDKVLAAWNGLMIAAFARGARQLGRAPYLDA
                     ACRAARFAKARLWNPEARMLFRRWRAGAVSVEGFAEDYACLVWGLIELYEAGGGSEWL
                     DWALDLQHRQDDLFWDRSSGGWFATSGRDRSLIVRMKDGHDGAEPSATSVGVRNLLAL
                     DGAQGERRREADIALIFRGSAARLAEAGRSVPMLLAALSEWHGRRRRLVVRAGARRPQ
                     GGGRAGI"
     gene            865..2037
                     /locus_tag="PLT35_13405"
     CDS             865..2037
                     /locus_tag="PLT35_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30269.1"
                     /translation="MLAFRVRRFRVIRLAVLVGLAAALAAPAPAQSLAVVVPPTDQAR
                     AAWQALEEARYKDAEAAFTRALASRPDDAALLVGSAIVARRLGRAVDARDALTRALRI
                     DPALTPASQLLGTLLHEAGDVEGAIRVYEAALVRAPDHEQLLARLEDWRKESSLHDQF
                     QQTRGGHFAVLFEGRAEEQLASAAVDILEAAYARVGDYLLVYPPEPVTVVLYTQQQFR
                     DVTRTPGWSGGLFDGRIRLPVRGGLDNREELERVLTHEYVHALLFSAAPTGVPAWLNE
                     GLATALEPGGLERAEHELSRAQAAIPLKDLRRSFASLPADRVALAYGVSALAVQALLD
                     RAGPARVMGLLRDLSAGEPLEPAFESWIQISLGDFEREWAACAVASARAPRPRGPR"
BASE COUNT          245 a          752 c          796 g          317 t
ORIGIN      
        1 aaggcctcgg agagcacttc gggatcgagc gccatggcaa cgcgccgatc gatccgcacg
       61 gcgagttcgc cggccggaac ctgctgcatg tcgccattcc ggccgaggcg ctcgcggccc
      121 ggcaccggct cccgctcggc caggtcaggc ggctgctcgc cggcgcgaag gccgcgatgc
      181 tcgccgcgcg cgccgcgcgc ccgaggccgc ttcgcgacga caaggtgctt gcggcctgga
      241 acgggctgat gatcgcggcc ttcgcgcgcg gcgcgcgcca gctggggcgc gcgccttacc
      301 tggacgccgc gtgccgcgcg gcgcggttcg cgaaggcgcg cctctggaat ccggaggcgc
      361 gaatgctctt ccgtcgctgg cgcgcgggcg cggtctcggt cgagggcttc gccgaggact
      421 acgcctgtct ggtctggggg ctgatcgagc tgtacgaagc tggcggcggc tccgagtggc
      481 tcgactgggc gctcgacctc cagcaccgac aggacgacct cttctgggac cggtcgtcgg
      541 gcggctggtt cgcgacctcg ggccgggatc gctcgctcat cgtccgaatg aaggacgggc
      601 acgacggcgc ggagccctcg gccacctcgg tcggtgtgcg gaacctcctg gcgctcgacg
      661 gcgcgcaggg agagcggcgc cgcgaagccg acatcgcgct gatcttccgc ggctcggcag
      721 cgcgcctggc ggaggccggc cggagcgtgc cgatgctgct cgcggccttg tccgagtggc
      781 acggccggcg ccgccgcctc gtcgtgcggg ccggcgcgcg ccgcccgcag gggggaggcc
      841 gcgcggggat atgatggggg cggcatgctc gcgtttcgag tccggcgctt tcgcgtgatc
      901 cggctggccg tgctggtggg tctggcggcg gcgctcgccg cccccgcgcc ggcacagtcg
      961 ctggcggtcg tggttcctcc gaccgaccag gcccgcgcgg cctggcaggc tctcgaggag
     1021 gctcgctaca aggatgccga ggcggccttc acacgggccc tcgcgtcgag gccggacgat
     1081 gccgcgctgc tcgtcgggtc ggctattgtc gcccggcgcc tcggccgcgc cgtggacgcg
     1141 agagacgccc tcacgcgcgc cctgcggatc gatccggcgc tgacgccggc gtcgcagctc
     1201 ctgggcacgc tgctccacga ggccggcgac gtcgagggcg cgatccgggt ctacgaggcc
     1261 gccctcgtcc gcgcgccgga tcacgagcag ctgctcgcgc gtctcgagga ctggaggaag
     1321 gaatcctccc ttcatgacca gttccagcag acccggggcg ggcactttgc cgtgctcttc
     1381 gagggccggg cggaagagca gttggcctcg gcggccgtcg acatcctcga agccgcctat
     1441 gcgcgcgtcg gcgattacct gctggtctat ccgccagaac ccgtcacggt cgtcctctac
     1501 actcagcagc agtttcgcga cgtcacccgt acgcccggat ggagcggggg gcttttcgat
     1561 gggcggattc gtcttcccgt ccggggcggc ctggacaatc gcgaggaact cgagcgggtg
     1621 ctgacccacg agtacgtcca cgcgctgctg ttcagcgcgg cgccgaccgg cgtgccggcg
     1681 tggctcaacg aggggctggc cacggccctc gagccgggtg gcctcgagcg ggccgaacac
     1741 gaactgtcgc gggcgcaggc ggcaatcccg ctcaaggacc tgcggcgctc gttcgcgtcg
     1801 ctgccggccg atcgcgtggc ccttgcctac ggcgtgagcg cgctggccgt gcaggcgctg
     1861 ctggatcggg ccggtcctgc gcgggtcatg gggcttctga gagacctgtc ggcaggcgag
     1921 ccgctcgagc ccgccttcga gtcctggatc cagatttcgc tcggtgattt cgagcgggaa
     1981 tgggccgcat gcgccgtggc gtcggcccgg gcgcctcgcc cacgcggccc gcgctgagtc
     2041 cggctctcgg tccggcggcc gcacgccgcg tcagtacttc gagatcgtca cgccggcgtc
     2101 ggccagcagc
//
LOCUS       DAOSIF010000527         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_45640_length_2102_cov_3.626771, whole genome shotgun sequence.
ACCESSION   DAOSIF010000527 DAOSIF010000000
VERSION     DAOSIF010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45640_length_2102_cov_3.626771"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /gene="argS"
                     /locus_tag="PLT35_13410"
     CDS             <1..1805
                     /gene="argS"
                     /locus_tag="PLT35_13410"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG30270.1"
                     /translation="DHLRLRKRAHHASARLVTGVAVHDQLGDHRIVEGRNRVPCAHTA
                     INPNKAAHIGHLRNCALGDTLVRSLRFLGHAVEIQNYIDDTGVQVADVVVGFRHLEGR
                     SAADVEAIARDTRFDHYCWDLYARVTGWYEADKARLAVRAEALHEIERGEGESAAIGR
                     LVADRIVRCHLKTMARLNVRYDLLTWEGDILRLQFWSKAFERLKADGAVYFQGSGRLA
                     GCWVMRIEDGIAHAPRGAEAAEGALTREDAGEDAEQAEKVIVRSNGTVTYVGKDIAYQ
                     FWKFGLLGEDFHYRLFGETGHDGPLWATSSVATPEEAALRPSFGRARAVYNVIDVRQS
                     YLQRLLSQALAVAGHPDEAERSHHFAYEMVALSHQTARHLGYAPEAEEAGRPFVEVSG
                     RKGLGVKADDLVDTLIEKALAEVTARHPELPEPERRRTAETIAVAAVRYFMVRFSRGK
                     VIAFDIDEALSFEGESGPYLQYSVVRAANIFQKLRERDGLDEAAVLGGLDGLAIEELA
                     GNDDASGVWALVLEAARLDEIAQQAVRSLEFSGLAKWAFALAQLFNAFYHRYPVLNEE
                     RRDAKLWRAAGVAYFRMQMTRALDLMGIEVPARM"
     gene            1827..>2102
                     /locus_tag="PLT35_13415"
     CDS             1827..>2102
                     /locus_tag="PLT35_13415"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOG30271.1"
                     /translation="MPLSKPRPRIAIAPCRAMSDYEESVRRAGGSVWILDPKADSAAD
                     VVASCGGLLLAGGADVAPERFGQLRHPAVTDVDDARDEYEVALVRAAL"
BASE COUNT          330 a          718 c          755 g          299 t
ORIGIN      
        1 ccgaccacct gcgtctgcgc aagcgcgcgc accatgcgag cgcgcgcctg gtcacgggtg
       61 tcgccgtgca cgatcagctt ggcgatcatc ggatcgtaga agggcgaaat cgagtcccct
      121 gtgcgcacac cgccatcaac ccgaacaagg ccgcccacat cggccacctg cgcaactgcg
      181 cccttggcga cacgctggtg cgatcgctgc ggttcctcgg acacgcggtg gagatccaga
      241 actacatcga cgacaccggc gttcaagtgg ccgacgtggt cgtcgggttc cggcatctcg
      301 agggccggag cgcggccgat gtcgaggcga tcgcgaggga cacccggttc gaccactact
      361 gctgggacct ctacgcgcgc gtaaccggct ggtacgaagc cgacaaggcg cgcctggccg
      421 tgcgggccga ggcgctgcac gagatcgaac gcggcgaggg cgaatcggcc gcgatcggcc
      481 gcctcgtcgc ggatcgtatc gtccggtgcc acctgaaaac gatggcgcgg ctgaacgtgc
      541 gctacgatct gctgacgtgg gaaggcgaca tcctgcgcct ccagttctgg agcaaggcct
      601 tcgagcgtct caaggccgac ggcgccgtgt acttccaggg cagcggccgg ctggcgggat
      661 gctgggtcat gcggatcgag gacggcatcg cccacgcgcc gcgcggggcg gaggccgccg
      721 agggggcact gacgcgcgag gacgccggcg aggacgccga acaggcggag aaggtgatcg
      781 tccgttcgaa cggcacggtg acctacgtcg gcaaggacat cgcgtaccag ttctggaagt
      841 tcggcctcct cggcgaggac ttccactacc ggctcttcgg cgagaccggc cacgacgggc
      901 ccctctgggc cacctcgtcg gtggcgacgc ccgaggaagc cgcgctgcgc ccgagcttcg
      961 ggcgggcccg cgccgtctac aacgtcatcg acgtccgcca gtcctacctg cagcgactcc
     1021 tgtcgcaggc gctggccgtg gccggacacc ctgacgaggc cgagcgctcg catcacttcg
     1081 cgtacgagat ggtggcgctc tcgcaccaga ctgcgcggca cctcggctac gcgccggaag
     1141 ccgaggaggc gggccggccc ttcgtcgagg tgtcgggccg gaaagggctc ggcgtcaagg
     1201 ccgacgatct ggtcgacacg ctgatcgaga aggcgctggc ggaagtgacg gcgcgccatc
     1261 ccgaactgcc ggagccggag cggcgccgca ctgccgagac gatcgccgtg gccgcggtcc
     1321 ggtacttcat ggtgcgcttc tcgcggggaa aggtcatcgc gttcgacatc gacgaggcgc
     1381 tcagcttcga gggcgagagc gggccgtacc tgcagtattc agtcgtccgc gccgcgaaca
     1441 tcttccagaa gctccgggag cgcgacggcc tggacgaagc cgccgtgctc ggcgggctgg
     1501 acgggctggc aatcgaagag ctggccggca atgacgacgc cagcggcgtc tgggcgctcg
     1561 tcctcgaggc cgcgcggctc gacgagatcg cccagcaggc cgtgcgctcg ctcgagttct
     1621 ccgggctcgc caagtgggcg ttcgcgctcg cgcagctgtt caacgccttc taccaccggt
     1681 acccggtgct caacgaggag cggcgggacg cgaagttgtg gcgcgccgca ggcgtggcgt
     1741 acttccgcat gcagatgacg cgcgccctcg atctgatggg catcgaagtg ccggcgagga
     1801 tgtaggctgt tgctggagga ccgtgcatgc cgctctcgaa gccgaggccc cgaatcgcca
     1861 tcgccccctg ccgcgccatg tcggactacg aggagtccgt ccgccgggcg ggcggcagcg
     1921 tctggatact ggaccccaag gcggactccg ccgccgacgt cgtcgcctcc tgcggagggc
     1981 tgctcctggc cggcggcgcg gacgtcgccc ccgagcggtt cggccagctg cgccaccccg
     2041 ccgtcacgga cgtggacgac gcgcgcgacg aatacgaggt ggcgctcgtg cgagcggccc
     2101 tg
//
LOCUS       DAOSIF010000528         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_46019_length_2090_cov_3.913514, whole genome shotgun sequence.
ACCESSION   DAOSIF010000528 DAOSIF010000000
VERSION     DAOSIF010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46019_length_2090_cov_3.913514"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLT35_13420"
     CDS             <1..997
                     /locus_tag="PLT35_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30272.1"
                     /translation="RGAGGAYRIGVRAAAPRPSSPGSYWNRIRLQRLGAGRYEWTVSE
                     ELAVGPVAPSALALALDVVFRAAERMESRDSRSAVAAGFPRTASTLGLLLRLDTLALE
                     RDARGSTYVRLGVRVTPAGIKDFAPRGAAYIEKYAGPLTLQGALVDAQGATWWTIEAA
                     DRLWTLRLRIRDGSLVPLAGASDRRLPARLRAVGDISTRMGRFGVGAKGVTADVSLTR
                     TGDEKGISARLRREPDWKLPFLVETLLDSPLAYPFEDAGTQVDWAVRATDRGTILTGL
                     YRTRVRETWLLRWLGGLMGNAIDEFRAGAEQEVDRYLRDCLLALRDDVTALAAAP"
     gene            complement(1046..>2090)
                     /locus_tag="PLT35_13425"
     CDS             complement(1046..>2090)
                     /locus_tag="PLT35_13425"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HOG30273.1"
                     /translation="VVLLVLVGTVFVTGPAKLLAGLTPGAVSYNAWLGVVLVYYFLAT
                     MLPIDKLIGRLYPVFGLILLVMAVGIAGGLVVEGYALPAFTLDNLHPAGLPVWALLFV
                     SIACGAISGFHATQSPLMARCLTTERYGRRVFYGAMIGEGIIALIWAAAGMAFYGGTP
                     GLGDALRAGGPGGAVHTICMALLGPFGGVLAILGVVVCPITSGDTAFRSARLTLADWI
                     RLDQRPIGRRLLIAVPMFAAGAALSQMNFDVVWRYFAWSNQTLAMLVLWTGAVYLVRA
                     GKPHWFASVPAAFMTAVSATYLLQAPEGFNLPAAIAYPAGLAGAAAVSGAFVGIVFRR
                     NAGRAADLAASDA"
BASE COUNT          311 a          762 c          755 g          262 t
ORIGIN      
        1 ccgcggcgcc gggggcgcgt acaggattgg cgtgcgcgcc gccgccccgc gcccgtcgag
       61 cccgggttcg tactggaacc gcattcggct tcagcgcctg ggagccggcc gctacgaatg
      121 gactgtgagc gaggaactgg ccgtcggccc cgtggcgccg tcggccctgg cgttggcgct
      181 cgacgtcgtg ttccgcgccg ccgagcgcat ggagagccgc gactcccgct ccgccgtcgc
      241 cgccggcttt ccgcggacgg catcgacgct cggcttgctt ctgaggctcg acacgctggc
      301 gctggagcgg gacgcgcgcg gctcgaccta cgtgcgcctc ggcgtgcgcg tgacgccggc
      361 cggcatcaag gacttcgcgc cgcgcggcgc ggcgtacatc gaaaagtacg cggggccgct
      421 gacgctccag ggcgcgctcg tcgatgccca gggcgcaacg tggtggacca tcgaggcggc
      481 agatcgcctc tggacgctgc gcctccgcat cagggacggc agcctcgtgc cgctggccgg
      541 ggcgtccgac cgccgcctcc cggcccgcct gcgggcggtc ggcgacatct cgacgcgcat
      601 gggccggttc ggcgtcggcg cgaagggagt cacggccgat gtgtcgctga cgaggacggg
      661 cgacgagaag ggcatctccg cccggctccg tcgcgagccg gactggaagc tgccgtttct
      721 cgtcgagacc ctgctcgatt cgcccctcgc gtacccgttc gaggacgcgg gaacgcaggt
      781 ggactgggcc gtccgggcca ccgatcgcgg cacgatcctg accggcctct accggactcg
      841 cgtgcgcgag acgtggctgc tgcgctggct cgggggcctg atgggaaacg ccatcgacga
      901 attccgcgcc ggggccgagc aggaagtcga tcgctacctg cgcgattgcc tcctggcgct
      961 gcgagacgac gtcacggcgc tcgccgccgc gccgtgagcg gccgcccggc cctccggcgg
     1021 gcggggcccg cctgtcgtgc ggcgcctacg cgtccgatgc cgccaggtcc gccgcccggc
     1081 ccgcgttcct cctgaagacg atgcccacga aggccccgga aaccgccgcc gcgccggcga
     1141 gtcccgccgg ataggcgatt gccgccggca ggttgaagcc ttccggcgcc tgcagcaggt
     1201 aggtggccga gacggccgtc atgaacgccg ccggcacgct cgcgaaccag tgcggcttgc
     1261 cggcgcgcac gaggtacacc gcgccggtcc acagcaccag catggccagc gtctggttcg
     1321 accaggcgaa gtaccgccag acgacgtcga agttcatctg ggacagcgcc gcgcccgcgg
     1381 cgaacatcgg gaccgcgatc aacaaccgtc tcccgatggg ccgctgatcg aggcggatcc
     1441 aatcggccag cgtcaggcgc gcgctgcgga acgccgtgtc ccccgacgtg atggggcaga
     1501 cgacgacgcc gaggatggcc aggacgccgc cgaagggtcc cagcagggcc atgcagatcg
     1561 tgtggacggc gccgcccggc ccgccggccc tcagcgcgtc gccgaggccg ggcgtgccgc
     1621 cgtagaacgc catgccggcc gcggcccaga tgagcgcgat gatcccctcg ccgatcatgg
     1681 ccccgtagaa gacgcgccgc ccgtagcgct ccgtcgtcag gcacctggcc atgagcggcg
     1741 actgcgtggc gtggaagccg ctgatcgccc cgcaggcaat cgacacaaac agcagcgccc
     1801 acaccggcag ccccgccggg tgcaggttgt ccagggtgaa cgccggcaga gcgtagcctt
     1861 cgacgacgag ccctccggcg atgccgacgg ccatgacgag gaggatcagg ccgaacaccg
     1921 ggtacagccg cccgatcagc ttgtcgatcg gcagcatcgt cgcgaggaag tagtagacca
     1981 ggacgacacc gagccacgcg ttatagctga cggctcccgg ggtgaggccg gcgagcagct
     2041 tcgccggccc ggtgacgaag accgtgccga cgagcacgag cagcaccacg
//
LOCUS       DAOSIF010000529         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_46291_length_2081_cov_3.470385, whole genome shotgun sequence.
ACCESSION   DAOSIF010000529 DAOSIF010000000
VERSION     DAOSIF010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46291_length_2081_cov_3.470385"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLT35_13430"
     CDS             <1..988
                     /locus_tag="PLT35_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOG30274.1"
                     /translation="AGWTGEAEWRGYLDESEWPVSYNPARGYLASANNKVAPDSFPFL
                     LGTSWDAPYRAARIVELLEERPRLSAEDFARMQGDLRSAQVAAILPFLLTARPLGPPG
                     RDAMALLRKWNGTLDGASPEAALFEAWYDATVRALFADELGDDLAAEYVLRRSSAAKA
                     IDSLVQSGDSAWCDDVRTPEPETCGTLLGRTLQQALAEMGGRQGTSNVTKWRWDRVNA
                     ARFPHAPLDAVPVLRRYFSRTVPHGGDAFTLTPVSPIGGDIFVSSYRQIVDLAGPDAS
                     RFMIPMGQSGHVWSDRYANLLDPWRRVEYIPMRFTRAAVDDAARATLVLEQR"
     gene            1041..>2081
                     /locus_tag="PLT35_13435"
     CDS             1041..>2081
                     /locus_tag="PLT35_13435"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOG30275.1"
                     /translation="MDARIRPAGLHTTAVHAGEAPDPATGALATPIHLSTAFHLGTAE
                     RGAAVFSGREEAYVYSRWANPTVSAFERKAAALEGAEAAIATASGMAAIASAIFTSVQ
                     SGDHIVSATAIYPSTYHLMATQLASMGVRTTFVDATDPGNVARAIGPSTKLVYLETPG
                     NPTLALCDLEAIGRIARSAGLATICDNTFASPVNQRPLELGIDTVVHSATKYFCGHGD
                     AVGGLVLGRAAFIDRCLKEPLRYYGGIMAPFSAYLMLRGAATMPLRVMRQNSSALRIA
                     EWLERHRAVARVLYPGLPSHPQHALAKRQMPGGFGGIVCFDLAGGVEAGARLMNAVRV
                     CSLATSLGDARTL"
BASE COUNT          299 a          750 c          744 g          288 t
ORIGIN      
        1 tgcgggctgg acgggcgaag cggaatggcg aggctacctc gacgagagcg agtggcccgt
       61 gtcctataac ccggcgcgcg ggtacctggc gtcggccaac aacaaggtgg cgcccgactc
      121 gttcccgttc ctgctcggca cgagctggga cgcgccgtac cgcgcggcgc ggatcgtgga
      181 gcttctcgag gagcgcccgc gcctttccgc cgaggacttc gcgcgcatgc agggcgacct
      241 gcgatcggcg caggtcgcgg cgatcctgcc gttcctgctg accgcccggc cgctcggccc
      301 tcccggccgc gacgcgatgg cgctgctgcg caagtggaac ggcaccctcg acggcgcgag
      361 ccccgaggcc gcgctcttcg aggcctggta cgacgcgacc gttcgcgcct tgttcgcgga
      421 cgaactgggc gacgacctgg ctgccgagta cgtgctgcgc cgcagctccg ccgccaaggc
      481 catcgacagc ctcgtgcagt ccggcgattc cgcatggtgc gacgacgtgc gcacgccgga
      541 gccggaaacg tgcgggaccc tgctggggcg gacgctgcag caggccttgg cggagatggg
      601 cggccggcag ggcacgtcga acgtcacgaa gtggcggtgg gaccgcgtga acgccgcgcg
      661 cttcccccac gcgccccttg acgccgtgcc ggtcctcagg cggtacttca gccgcaccgt
      721 cccgcacggc ggcgacgcgt tcacgctgac gcccgtcagc cccatcggcg gcgacatctt
      781 cgtgtcctcg taccgccaaa tcgtcgacct cgccggaccg gacgcctcgc gcttcatgat
      841 ccccatgggg cagtccgggc acgtctggag cgaccggtat gcgaacctgc tcgatccatg
      901 gaggcgggtc gagtacatcc cgatgcgctt cacccgcgcc gcggtggacg atgcggcgag
      961 ggcgacgctg gtgctcgagc agcgctgagc ccctgcggag ggacgggacg acggcgcgag
     1021 gggatcgcag ggagacgcgc atggacgcac gcatccggcc agccggcctt cacaccaccg
     1081 cggtgcacgc gggcgaggcg ccagacccgg ccacgggcgc cctggccacg cccatccacc
     1141 tgtccacggc gttccacctg ggcaccgccg agcgcggcgc ggccgttttc tcgggccggg
     1201 aggaagccta cgtgtactcg cggtgggcca atccgacggt gagcgccttc gagcgcaagg
     1261 ccgccgccct cgagggcgcc gaggcggcaa tcgcgaccgc cagcggcatg gcggccatcg
     1321 cgtcggccat cttcaccagc gtgcagagcg gcgaccacat cgtgtcggcg acggcgatct
     1381 acccgagcac ctaccacctg atggccacgc agctggcgtc gatgggcgtc cgcacgacgt
     1441 tcgtggacgc cacggacccg ggcaacgtag cgcgagcgat cgggccatcc acgaaactcg
     1501 tgtatctgga gacacccggc aaccccacgc tcgcgctctg cgacctcgag gcaatcggga
     1561 ggatcgcccg ttcggccggt ctcgccacga tttgcgacaa caccttcgcg tcgccggtga
     1621 accagcgccc cctcgaactg gggatcgaca cggtcgtgca cagcgcaacg aagtacttct
     1681 gcggccacgg cgatgccgtg ggcgggctgg tcctggggcg tgcggcgttc atcgatcggt
     1741 gcctgaagga gccgctccgg tactacggcg gcatcatggc gccgttcagc gcgtacctca
     1801 tgctgcgggg cgcggcgacg atgccgctga gggtcatgcg ccagaactcg agcgccctgc
     1861 gcatcgcgga gtggctggag cgccaccggg ccgtcgcgcg ggtgctctac cccggcctgc
     1921 cgtcgcatcc gcagcacgcg ctggcgaagc ggcagatgcc gggcggcttc ggcggcatcg
     1981 tgtgcttcga tctggcgggc ggcgtggagg ccggcgcccg gctcatgaac gccgtgcgcg
     2041 tgtgctcgct cgcgacgagc ctcggcgacg cgcgcacgct c
//
LOCUS       DAOSIF010000530         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_46354_length_2079_cov_3.041008, whole genome shotgun sequence.
ACCESSION   DAOSIF010000530 DAOSIF010000000
VERSION     DAOSIF010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46354_length_2079_cov_3.041008"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLT35_13440"
     CDS             <1..901
                     /locus_tag="PLT35_13440"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOG30276.1"
                     /translation="SMAITPEVIAMVEECIPLAPLHNPPNLVGIRVAQELFPGLPQVG
                     VFDTAFHQTMPPVAYTYPVPHEWLEAHSVRRYGFHGTSHRFVAKQALHLTGLSPEKHR
                     LVTAHLGNGCSCAAVRDGKSVDTTMGLTPLEGVMMGTRSGSVDPAIIDHVAKALGKPE
                     REILDALNKKSGLLGVSGLSNDMRTIEDAAARGHERARLAFDIFCYVLAKSLAALVVP
                     LGHLDALVFTGGIGENSVAVRARTVEHLGFLGLTIDPHANATHGRSQNGRITEEIRPQ
                     ALVVPTNEELMIAQDTAEIARRR"
     gene            939..>2079
                     /locus_tag="PLT35_13445"
     CDS             939..>2079
                     /locus_tag="PLT35_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOG30277.1"
                     /translation="MATTILVAPAGRRVGLTTACLGLVRALDRQGVRVSFVKPIANRE
                     VSQSTELVALGAHILPVKSVPIHTAEDLLAAGDDQTLMEHVVELAGKASVGADVLIVE
                     GMNPEPGMVHASRVNGLMFKALDAELVLVAAPRSQNALEVADAIAIAAHGYGPQPEGR
                     PVRCILNRVCVGAAGQRAETETIGQAQGGCSDCEGVSTFAENEAAHREALAAAKLVPL
                     AVVPCSNDLAAPRVLDVAHALKARVLFAGEIRTRRVSDVGICAATVPNALRAMRPGAL
                     VITPGDRSDVIMAVALSVQTGTPIAGLLLTGGLDPDPRIIRLCRKALDTGLPVLAVDD
                     WSYRAANRVGGMNPEIPADDAERIERTMNYVADRIDVEWVKGFGHT"
BASE COUNT          314 a          723 c          755 g          287 t
ORIGIN      
        1 gtcgatggcg atcacgcccg aggtcattgc catggtcgag gagtgcattc ccctcgcgcc
       61 gctgcacaac ccgccgaacc tggtgggcat ccgcgtggcc caggagctgt tcccgggtct
      121 gccgcaggta ggcgtgttcg acacggcgtt ccaccagacg atgccgcccg tcgcctacac
      181 gtatcccgtg ccgcacgagt ggctcgaggc gcacagcgtg cggcggtacg ggttccacgg
      241 gacgagccac cggttcgtgg cgaagcaggc gctccacctg accggcctct cgcccgagaa
      301 gcaccggctc gtcaccgccc acctgggcaa cgggtgttcc tgcgcggcgg tgcgcgacgg
      361 caagagcgtg gacacgacca tggggctcac gccgctcgag ggcgtgatga tgggcacgcg
      421 cagcggctcg gtggacccgg cgatcatcga ccacgtggcg aaggccctcg gcaagccgga
      481 acgggagatc ctggacgcgc tcaacaagaa gtccggcctg ctcggcgtgt cgggcctctc
      541 gaacgacatg cggacgatcg aggacgcggc cgccaggggc cacgagcgcg cgcggctggc
      601 cttcgacatc ttctgctacg tgctggccaa gtcgctggcg gcgctggtgg tgccgctggg
      661 ccatctggac gcgctggtgt tcaccggcgg gatcggggag aactcggtgg cggtccgcgc
      721 gaggaccgtg gagcacctcg gcttcctcgg cctgaccatc gacccgcacg cgaacgcgac
      781 gcacggccgc agccagaacg ggcgcatcac cgaggagatc cggccccagg cgctcgtcgt
      841 gccgacgaat gaagaactga tgatcgcgca ggacacggcg gagatcgcgc gcaggagata
      901 atccggcggc gtcattcacg tcatttcggg aggttccgtt ggccaccacc atcctcgttg
      961 ccccggccgg gcggcgcgtc ggactgacga ctgcctgcct cggtcttgtc cgggcgctcg
     1021 accgtcaggg cgtccgcgtg tcgttcgtca agccgatcgc gaacagggaa gtcagccagt
     1081 ccacggagct cgtggcgctc ggcgcgcata ttctgccggt caagtccgtg ccgatccaca
     1141 ccgcggagga cctgttggcg gccggcgacg accagacgct catggagcac gtcgtcgagc
     1201 tggcggggaa ggcctcggtc ggggccgacg ttctgatcgt cgaggggatg aacccggagc
     1261 ccggcatggt gcacgcgtcc cgggtcaacg ggctcatgtt caaggccctc gacgcggagc
     1321 tcgtgctggt ggcggcgccc cgcagccaga acgcgctcga ggtcgccgac gcgatcgcca
     1381 tcgccgcgca cggctacggg ccccagcccg agggccggcc ggtgcgctgc atcctcaacc
     1441 gggtgtgcgt cggggccgcg ggtcagcggg ccgagaccga gacgatcggg caggcgcagg
     1501 gcggctgctc cgactgtgag ggcgtcagca cgttcgcgga gaacgaggcg gcgcaccgcg
     1561 aggcgctggc ggccgcgaag ctcgtgcccc tcgccgtcgt gccgtgcagc aacgacctcg
     1621 cggcgccgcg cgtcctcgac gtcgcccatg cgctcaaggc gcgggtcctc ttcgcagggg
     1681 agatccggac gcgccgcgtc agcgacgtcg gcatctgcgc ggcgaccgtg ccgaacgccc
     1741 tccgggcgat gcggcccggc gccctcgtca tcaccccggg ggaccggagc gacgtcatca
     1801 tggccgtcgc cctgagcgtc cagaccggca cgccgatcgc cggcctcctg ctgacggggg
     1861 gcctggaccc ggatccgcgg atcatccggc tgtgccgcaa ggcgctcgac accgggctgc
     1921 ccgtgctggc cgtggacgac tggagctacc gcgccgccaa ccgcgtgggc ggcatgaacc
     1981 ccgagatccc cgccgacgat gccgagcgga tcgagcggac gatgaactac gtcgccgatc
     2041 ggatcgacgt cgagtgggtg aagggattcg ggcacacga
//
LOCUS       DAOSIF010000531         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_46493_length_2074_cov_4.016345, whole genome shotgun sequence.
ACCESSION   DAOSIF010000531 DAOSIF010000000
VERSION     DAOSIF010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46493_length_2074_cov_4.016345"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLT35_13450"
     CDS             <1..339
                     /locus_tag="PLT35_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30278.1"
                     /translation="DLLLELLDLALVKFQFQMSAITAGFVGAEYFRKYPGRFNSMHLQ
                     DVDMNAPAPPPGPNGRPGRRPQVAVGRGSIDWVATFTAAKAAGVRSYYVEQNWELTEQ
                     SVAYLKTLAV"
     gene            complement(346..422)
                     /locus_tag="PLT35_13455"
     tRNA            complement(346..422)
                     /locus_tag="PLT35_13455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(386..388),aa:Arg,seq:ccg)
     gene            complement(436..509)
                     /locus_tag="PLT35_13460"
     tRNA            complement(436..509)
                     /locus_tag="PLT35_13460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(473..475),aa:Arg,seq:tcg)
     gene            659..1960
                     /locus_tag="PLT35_13465"
     CDS             659..1960
                     /locus_tag="PLT35_13465"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOG30279.1"
                     /translation="MGVTPAQKSLPESHGSVPIPANGGFFRKLLAFSGPGYLVAVGYM
                     DPGNWATDIAGGARFGYTLLSVILLSNLMAILLQALAVRLGVGTSYDLAQACRAYYSR
                     PVSFVLWILAEVAIAACDLAEVIGSAVALGLLFGIPLAAGVVITALDVFIVLFVQQHR
                     FRILEGIVLTLILGIAGGFAIELFLSKPDLGGVLRGFVPSAEILRDPDMLYIAAGILG
                     ATVMPHNLYLHSSVVQTRKYADTDEGRGEAIRFATIDSTVALMSALFINGAILVVSAA
                     TFHGTQYEWVADITDAHKLLAPILGTTLASTLFAVTLLFSGQNSTLTGTLAGQIVMEG
                     FLDLRLRPWLRRLVTRSIAIVPALVAVMLYGDAGIGRLLILSQVVLSLQLPFAVIPLV
                     AFTSDRRKMGPAVAPPWMRALAWAVAALIAGLSVWLLVQVL"
BASE COUNT          303 a          703 c          716 g          352 t
ORIGIN      
        1 gacctgctgc tggagctgct cgacctggcg ctggtgaagt tccagttcca gatgtccgcg
       61 atcacggccg ggttcgtcgg cgccgagtac ttccggaagt acccgggccg gttcaactcg
      121 atgcacctcc aggacgtgga catgaacgcc ccggcgccgc ctcccgggcc gaacgggcgg
      181 ccgggccggc gcccgcaagt ggcggtgggc aggggcagca tcgactgggt ggcgacgttc
      241 acggcggcga aggccgcggg cgtgcgcagc tactacgtcg agcagaactg ggagctgacc
      301 gagcagagcg tcgcctacct caagacgctc gccgtgtagg gagggtggtg cgcccggggc
      361 gattcgaacg cccggccttt ggctccggag gccaacgctc tatccagctg agctacgggc
      421 gcaaagagca ggaaacgcgc ccggcaggac tcgaacctgc gacctacgga ttcgaagtcc
      481 ggcgctctat ccagctgagc tacgggcgca cggaatgcga tgatagcaca ccgggggctg
      541 gggatcgggg gttgggaacc gggagaactc gctgagccag agcgcgcgca tcgaagcggc
      601 ggtccccaac ccccaatccc caaccccgag ccccgagtcc ctgttgtaga ctcctcccgt
      661 gggtgtcaca cctgcccaga agagcctgcc cgagtcgcac gggtccgtcc cgattccggc
      721 aaacggaggg ttcttccgca aactgctggc gttctcgggc ccgggatacc tcgtcgccgt
      781 cgggtacatg gacccgggca actgggccac ggacatcgcc ggaggggcgc ggttcggcta
      841 cacgctgctg agcgtgatcc tgctgtcgaa cctgatggcc atcctgctgc aggcgctcgc
      901 ggtgcgcctt ggagtgggca cgagctacga cctcgcacag gcgtgccgcg cctactactc
      961 gcgcccggtg tcgttcgtcc tgtggatcct ggccgaggtg gccatcgccg cctgcgatct
     1021 cgcggaggtc atcgggtcgg ccgtcgcact cggcctgctt ttcggcatcc cgctcgccgc
     1081 gggcgtggtc atcaccgccc tcgacgtgtt catcgtgctc ttcgtgcagc agcaccgctt
     1141 ccgcatcctc gagggcatcg tgctgacgct catcctcggg atcgcgggcg ggttcgcgat
     1201 cgagctgttc ctgtcgaagc cggacctggg cggcgtgctc cgcgggttcg tgccaagcgc
     1261 ggagatcctg cgggacccgg acatgctcta catcgcggcc gggatcctcg gggcgacggt
     1321 gatgccgcac aacctctacc tccattcgtc ggtggtgcag acgcgcaagt acgcagacac
     1381 cgacgagggg aggggcgaag cgatccggtt cgcgacgatc gactcgacgg tggccttgat
     1441 gtcggcgctc ttcatcaacg gcgccatcct ggtcgtgtcg gcggcgacgt tccacggcac
     1501 ccagtacgag tgggtcgcgg acatcacgga cgcccacaag ctgctggccc cgattctcgg
     1561 cacgacgctg gccagcacgc tgttcgccgt gacgctcctc ttctcgggcc agaactcgac
     1621 gctgacgggc acgctggcgg gccagatcgt gatggagggg ttcctcgacc tccggctgcg
     1681 cccctggctg cgccggctcg tcacccgctc catcgccatc gtgccggcgc tcgtcgccgt
     1741 catgctgtac ggcgacgcgg ggatcgggcg gctgctcatc ctgagccagg tagtcctcag
     1801 cctgcagctg cccttcgcgg tgatcccgct cgttgcgttc acgtcggacc gccgcaagat
     1861 ggggccggcc gtcgcgccgc catggatgcg agctctggcc tgggcggtcg ccgcgctcat
     1921 cgcggggctc agcgtctggc tgctcgtgca ggtgctctga ggagtgggcg atgtacaagc
     1981 gtattctcgt ggccgtcgag cgctcggatg ccgaccgcac gatcatcgac cacgtgaagc
     2041 cgctggcgaa gatgtgcggc gccacgctgg tgct
//
LOCUS       DAOSIF010000532         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_46654_length_2069_cov_2.667329, whole genome shotgun sequence.
ACCESSION   DAOSIF010000532 DAOSIF010000000
VERSION     DAOSIF010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46654_length_2069_cov_2.667329"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..278)
                     /locus_tag="PLT35_13470"
     CDS             complement(120..278)
                     /locus_tag="PLT35_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30280.1"
                     /translation="MARADGAVFAARGRLGRLPGLARALVDRLAFAVRRPAEARLSLD
                     GGDTFVLV"
     gene            484..1386
                     /locus_tag="PLT35_13475"
     CDS             484..1386
                     /locus_tag="PLT35_13475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG30281.1"
                     /translation="MADQPLVTVVIPSYRRPDQLASCLRSVVRSDFPPDRFEVLVVDD
                     GNEEPLDARVNWSAGAVGVRWVRLPENLGPAAARNAGARAARGARLAFIDDDCLADRT
                     WLARLYASSAANDGAAVGGRVVGGSGDNLFCAADQAILDVVFAYYNEDPLQARFLSAA
                     SLLVPADAFRESGGFDPAFCTAEDREFCARWLAGGRRLVYAADAVAVHAATSSFGRFW
                     RRHFAFGEGAYRFRSRHARAPDGRIALEPPGFYRRLVSAPKKRPMRAREAAIAALIVV
                     SQLASALGFLAARRAAHTSKGRTA"
     gene            complement(1359..>2069)
                     /locus_tag="PLT35_13480"
     CDS             complement(1359..>2069)
                     /locus_tag="PLT35_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOG30282.1"
                     /translation="YPMVTLLSTNGQSLSAPRVQEALRAHPPTYLIVAIDGLTDATNT
                     VYRKGARLAPALDGVRALADWKQQTGARFPVLHCRFIAMRQNEHELPRLRGFAEEAGF
                     DMVSLRSLSIIDAAEDGHRGMVPAAERLRAYRYEHGRRVRHRDFVCQYAFSFPTVLAD
                     GTVVACDQDFNGREPYGVLRKGGSFRSIWTSRRASEVRRTIRDRPGEFSFCRNCPYAD
                     RPISSCSFDGYAVRPFEV"
BASE COUNT          262 a          759 c          756 g          292 t
ORIGIN      
        1 gcgaatggcg tcgatgatgg cctgaaggtt cgaccctcgg cccgagatca gcacgccgat
       61 gttgcggttc acgcggcctc ctgccagctg cccccggctc agcgcggggg gcgtgggcat
      121 cataccagca cgaacgtgtc cccgccgtcg agcgacaatc gggcctccgc cggccggcgc
      181 acggcgaagg cgagccgatc gacgagcgcc ctcgccaggc ccgggagccg acccaagcgg
      241 ccccgggctg cgaagacggc gccgtcggcc cgtgccaccg cccccgcctc gtgtgtacac
      301 tggctggatg aggcggcgct cccggggctg cgcgggacga ggcccagcag gacggccggc
      361 agatcgtcgc cggcgcggcc cgcgccgcat ccccctcgag ggccgccccc actcggaagc
      421 acccggaagg cgcacccgcg gccgcgggcg cgaggcccgg gtctgcggtt cgccggtgtc
      481 acgatggccg atcagcctct cgtcacggtt gtcattccca gttacaggcg ccccgaccag
      541 ctcgcctcct gccttcggtc ggtcgtgcgg tcggacttcc cgccagatcg cttcgaggtg
      601 ctggtcgtcg acgacggcaa cgaggagccg ctcgacgcgc gcgtgaactg gtcagcgggc
      661 gctgtcggcg tccgctgggt gcgccttcct gagaacctgg ggcccgccgc ggcgcgcaac
      721 gcgggggcgc gcgcggcccg cggggcccgc ctcgcgttca tcgacgacga ctgcctcgcc
      781 gaccgcacct ggctcgcgcg cctctacgcg tcgtcggccg ccaacgacgg cgccgcggtc
      841 ggcgggcgcg tagtcggcgg gagcggcgac aatctgttct gcgccgccga ccaggcgatc
      901 ctcgacgtcg tgttcgcgta ctacaacgaa gaccccttgc aggcgcggtt cctctcggcg
      961 gcgagcctgt tggtcccggc cgacgccttc cgcgagagcg gcggcttcga tccggcgttc
     1021 tgcacggccg aggaccgcga gttctgcgcg cgctggctcg ccggcgggcg gcggctggtg
     1081 tacgcggccg acgccgtggc ggtccacgcc gccaccagca gcttcggccg cttctggcgc
     1141 cggcacttcg cgttcggcga gggcgcgtac cggtttcgca gccggcacgc cagggcgcca
     1201 gacggtcgga tcgcgctcga gccgccgggg ttctaccggc gcctcgtgtc cgcgccgaag
     1261 aagcgcccga tgcgcgcgag ggaggcggcc atcgcggcgc tgatcgtcgt gtcccagctc
     1321 gcgagcgcgc tggggttcct ggcggcgcgg cgcgccgctc acacctcgaa gggccgcacg
     1381 gcgtagccgt cgaagctgca gctgctgatg ggccggtcgg cgtacgggca gttccggcag
     1441 aagctgaact cgcccgggcg atcgcggatc gtgcgccgaa cctcgctcgc cctgcggctc
     1501 gtccagatgc tgcggaacga tccgcccttc ctcagcacgc cgtagggctc acggccgttg
     1561 aagtcctggt cgcacgcgac gacggtgccg tcggcaagca cggtcgggaa gctgaaggcg
     1621 tactggcaaa cgaaatcgcg gtgtcgcacg cgccgcccgt gctcgtagcg gtacgcgcgc
     1681 aaccgctcgg cggccggcac catgccgcgg tgcccgtcct cggcggcgtc gatgatcgac
     1741 aggctgcgca gcgacaccat gtcgaacccg gcctcctcgg cgaacccgcg caggcggggc
     1801 agttcgtgct cgttctgccg catggcgatg aaccggcagt gcagcacggg gaatcgcgcg
     1861 ccggtttgct gcttccagtc cgccagggcc ctgacgccgt cgagcgccgg cgcgaggcgg
     1921 gccccctttc ggtacacggt gttggtggcg tcggtcagcc cgtcaatggc gacaatcagg
     1981 tacgtcggcg gatgagcgcg cagcgcctcc tgcacgcgcg gcgcgctgag gctctgcccg
     2041 ttggtcgaca gcagggtgac catcgggta
//
LOCUS       DAOSIF010000533         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_46699_length_2067_cov_3.012922, whole genome shotgun sequence.
ACCESSION   DAOSIF010000533 DAOSIF010000000
VERSION     DAOSIF010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46699_length_2067_cov_3.012922"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLT35_13485"
     CDS             <1..393
                     /locus_tag="PLT35_13485"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG30283.1"
                     /translation="FINNAVYGMTGGQMAPTTLPGQIATTCPLGRDVTTAGYPIRIVE
                     LLSTLRTPAYLTRVSVHEPKAIMNAKKAIRKAFKFQIDRVCFSLVEVVSTCPTGWGQQ
                     PHEACEWLEEHMLPYYPLGDVKTPEAAH"
     gene            413..973
                     /locus_tag="PLT35_13490"
     CDS             413..973
                     /locus_tag="PLT35_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG30284.1"
                     /translation="MHHELILAGFGGQGVLLIGKLLAYAGMRAGLEVTWMPAYGPEMR
                     GGTCNCTVVLSDRQVGSPVTSRPHGAIVLNLQSIDKFEPMVRPGGILVMNTSLINRPA
                     SRADLTVVPVAANQIAQEEGSVKAANMVALGAFLGASGIVSKDLVEGVLAETFAGRQK
                     IVQLNLACLAKGVEIGRTCARPPSAA"
     gene            989..2050
                     /locus_tag="PLT35_13495"
     CDS             989..2050
                     /locus_tag="PLT35_13495"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOG30285.1"
                     /translation="MAKKLMKGCEAIGEAAIQAGCRLFFGYPITPQNEIPEYMSGRLP
                     QVGGTFVQAESEVAASNMLYGAAGAGCRCLTSSSSPGISLMMEGVSYLAGAELPVVIV
                     NIMRGGPGLGGILPSQSDYFQATKGGGHGDYRLLVLAPSTVQEAADLTMEAFDLADKY
                     RNPVMILGDGLIGQMMEPVEFKPGIDPAALPDKAWATTGAAGRPPNIVNSLFLKAEEL
                     EQHNLDLGAKYRAMQSEVRCELYRADDSLDVLIVAYGTVARIARTSVDALREKGLKAG
                     LFRPVTLYPYPDEALARAVERARQVLVTELSMGQMVEDVRAIVAGRAPVAFYGRVGGM
                     VMTAEELAEQAEKLAAVPA"
BASE COUNT          343 a          691 c          725 g          308 t
ORIGIN      
        1 ttcatcaaca acgccgtcta cggcatgacg ggcggccaga tggccccgac gacgctgccc
       61 gggcagattg cgaccacctg cccgctcggc cgcgacgtca cgacggccgg ctacccgatt
      121 cgcatcgtcg agctgctctc gacgctccga acgccggcgt acttgacgcg ggtgtcggtg
      181 cacgagccga aggccatcat gaacgcgaag aaggccatcc ggaaagcgtt caagttccag
      241 atcgacaggg tgtgcttctc gctcgtcgag gtcgtgtcca cgtgcccgac aggctggggc
      301 cagcagcccc acgaagcctg cgagtggctc gaagagcaca tgctgccgta ctaccccctg
      361 ggtgacgtaa aaacgccgga ggccgcgcac tgatgccgag cgggaggaga ccatgcacca
      421 cgaactgatc ctggcgggtt tcggcggcca gggcgtgctg ctcatcggca agctgctcgc
      481 gtacgcgggc atgagagcgg gcctggaggt gacctggatg ccggcctacg gcccggagat
      541 gcgcggcggc acctgcaact gcaccgtcgt gctctcggat cggcaggtcg gctcgcccgt
      601 gacgagccgg ccccacggcg ccatcgtgct gaacctgcag tcgatcgaca agttcgagcc
      661 gatggtccgc cccggcggca tcctcgtgat gaacacgagc ctcatcaacc gccccgcgtc
      721 ccgcgccgac ctcacggtgg tgccggtggc ggccaaccag atcgcccagg aggaaggcag
      781 cgtcaaggcc gccaacatgg tcgctctggg cgcctttctg ggggcgtccg gcatcgtcag
      841 caaggacctg gtcgagggcg tactggccga gaccttcgcg ggccggcaga agatcgtgca
      901 gctcaacctg gcgtgcctgg ccaagggcgt cgagatcggg cggacctgcg cccgtccgcc
      961 ctccgccgcc tagccgcgga gatcgcacat ggcgaagaaa ctgatgaagg gctgcgaggc
     1021 catcggcgag gcggcgatcc aggctgggtg ccggctcttt ttcggctatc ccatcacgcc
     1081 gcagaacgag atcccggagt acatgtccgg ccgcctgccg caggtcgggg gcacgttcgt
     1141 gcaggccgag agcgaagtcg cggcctccaa catgctctac ggcgccgccg gcgccggctg
     1201 ccgctgtctc acctcgtcgt cgagccccgg catctcgctg atgatggagg gcgtgtcgta
     1261 cctggccggc gcggagctgc cggtcgtcat cgtcaacatc atgcgcggcg gccccggcct
     1321 cggcggaatc ctgccgtcgc agtcggacta tttccaggcc acgaagggcg gcggccacgg
     1381 cgactaccgg ctcctggtcc tcgcgccgtc caccgtgcag gaggcggccg acctgacgat
     1441 ggaggcgttc gacctggcgg acaagtaccg caatccggtg atgatcctcg gcgacggcct
     1501 gatcgggcag atgatggagc ccgtggagtt caaaccgggg atcgacccgg ccgccctgcc
     1561 cgacaaggcc tgggcgacaa ccggcgcggc agggcgcccg ccgaacatcg tgaactcgct
     1621 gttcctcaag gccgaggaac tcgagcagca caatctcgat ctgggcgcga agtaccgcgc
     1681 gatgcagagc gaagtgcgat gcgagctgta ccgggccgac gacagcctgg acgtgctcat
     1741 cgtggcctac ggcaccgtgg cgcgcatcgc gaggacatcg gttgacgcgc tgcgggagaa
     1801 gggcctgaag gccgggctgt tccgtcccgt gacgctctac ccctatcccg acgaggcctt
     1861 ggcccgggcg gttgagcggg ccaggcaggt gctggtcacc gagctgtcga tggggcagat
     1921 ggtggaggat gtgcgcgcca tcgtcgctgg gcgcgcgccg gtggcgttct acggccgggt
     1981 ggggggaatg gtcatgaccg ccgaagagct ggccgagcag gccgagaagc tcgcggccgt
     2041 tcccgcctga atcgcaaacc gcttcgg
//
LOCUS       DAOSIF010000534         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_46816_length_2063_cov_3.912849, whole genome shotgun sequence.
ACCESSION   DAOSIF010000534 DAOSIF010000000
VERSION     DAOSIF010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46816_length_2063_cov_3.912849"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLT35_13500"
     CDS             complement(<1..623)
                     /locus_tag="PLT35_13500"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG30286.1"
                     /translation="MHQTIRTCLGIAVLCAVVGATPAMAQNITTGTLTGTVMDAQQGV
                     LPGATVVAVHQPTGTTYEAVTQADGRFTMLAVRVGGPYTVSAEMTGFRKQEQAGVEVG
                     LGESRAVAFTLQLETVQETVNVVAAAQVIDTTRAGAAANVGTQTLEALPTISRSLNDF
                     ARTSPYFNTNTDSATGGEMVSVAGRSNRYNNMQIDGAVNNDVFGLAAT"
     gene            complement(867..>2063)
                     /locus_tag="PLT35_13505"
     CDS             complement(867..>2063)
                     /locus_tag="PLT35_13505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG30287.1"
                     /translation="QGLPPGGADASAGQAPAAPEQGAQNPTFRVTVDLVTSDVIARDA
                     RGQFVADLKKDDFELYEDGVLQDIASLVLVHGGRVSNLALPPPAAPPEGIILPPARPM
                     NDASGRIFILFIDDLHLDFKNTGRVRELLKKIKNTLIHQGDMFGVVSSGTSSIEIDLT
                     YDLKRIDEAIKKTMGGALKPTDIIQGPEGAEGPTEVRYRAHVAFSAAADMLENLSKVH
                     NRRKALIYVSNGYDFNPFQKSRYGEAEFFENRFGAAQLRQTQGDDTYGGTAQNQGDRY
                     DGNTPRGNDRWADADLVRELAELTRAANRANTTIYTIDPRGLVGMPDLDDKVDPVEWQ
                     EFVTNSINSLRVLAELTGGIAVVNQNDFEKALKRIDAETSDYCMLGYYSNNPDPLNGP
                     GRSTSR"
BASE COUNT          271 a          725 c          691 g          376 t
ORIGIN      
        1 gtggcggcga ggccgaacac gtcgttgttc accgccccgt cgatctgcat gttgttgtag
       61 cggttgctcc ggccggccac gctgaccatt tcgccgccgg tcgcgctgtc cgtgttcgtg
      121 ttgaagtacg gcgacgtccg ggcgaagtcg ttcaggctcc gcgagatcgt cggcagcgcc
      181 tcgagcgtct gcgtcccgac gtttgcggcc gccccggccc gggtcgtgtc gatgacctgc
      241 gccgccgcca cgacgttcac cgtctcctgc accgtctcga gctggagcgt gaacgccacc
      301 gcccgcgact cgccgaggcc cacctcgacg ccggcctgct cctgcttgcg aaacccggtc
      361 atttcagcgc tcacggtgta cggcccgccg acgcggaccg ccagcatcgt gaaccggccg
      421 tcggcctgcg tcacggcctc gtaggtggtg cccgtcggct ggtgcaccgc cacgacggtg
      481 gcccccggca gcacgccctg ctgggcgtcc atcacggtgc cggtcagtgt cccggtcgtg
      541 atgttctggg ccatcgcggg cgtggcgccg acgacggcgc agagcacggc gatccccagg
      601 caagtcctga tggtctgatg catcctgtct tctccgatag agagtgtcac tgggaacggc
      661 actcgacgcg cccggtgcga ccccgtcgca ggtggcggca cgcgccgcct gacggacccg
      721 gcccgagccg cacgagcata gcacaagagg acggccggcc ggtacggccg cggcggcgcc
      781 gcggcccctc ggccccgccc gctacctccg cgggttcgtc cggagcgagt acgacttcct
      841 cgaccagacg tcgatgtcct tccgcttcac cttgacgtcg atctgccggg tccgttcaag
      901 ggatcggggt tgttggagta gtagccgagc atgcagtagt cgctggtctc cgcgtcgatg
      961 cgcttcagcg ccttctcgaa gtcgttctgg ttgaccacgg cgatgccgcc cgtcagttcc
     1021 gccaggacgc gcaggctgtt gatcgagttc gtcacgaact cctgccactc gaccgggtcc
     1081 accttgtcgt cgagatcggg catgccgacc agcccgcgcg ggtcgatggt gtagatcgtc
     1141 gtgttggcgc ggttcgccgc ccgggtcagc tccgccagct cccggacgag gtcggcgtcg
     1201 gcccatcggt cgttccctcg cggcgtgttg ccgtcgtagc ggtcgccctg gttctgggcc
     1261 gtcccgccgt aggtgtcgtc gccctgggtc tgccgcagct gggccgcccc gaaacggttc
     1321 tcgaagaact ccgcctcgcc gtagcgcgat ttctggaacg gattgaaatc gtacccgttg
     1381 ctgacgtaga tgagcgcctt ccgccggttg tgcaccttcg agaggttctc gagcatgtcc
     1441 gccgcagccg agaacgcgac gtgcgcccgg taccggacct cggtgggccc ttccgccccc
     1501 tccgggccct ggatgatgtc ggtgggcttc agcgcgcccc ccatcgtctt cttgatggcc
     1561 tcgtcgatcc gcttgagatc gtacgtgaga tcgatctcga tcgacgaggt gcccgacgag
     1621 accacgccga acatgtcgcc ctggtgaatc agcgtgttct tgatcttctt gagcagctcg
     1681 cgcacccggc ccgtgttctt gaaatccagg tgcagatcgt cgatgaagag gatgaagatg
     1741 cggcccgagg cgtcgttcat cggccgggcc ggcggcagga tgatgccctc gggcggagcg
     1801 gccggaggcg gcagggccag gttcgagacc cggcctccgt gcaccagaac cagggaggcg
     1861 atgtcctgca gcaccccgtc ctcgtacagc tcgaagtcgt ccttcttcag gtccgccacg
     1921 aactgccccc gcgcgtcgcg ggcgatgacg tcgctcgtga ccagatccac ggtcacccgg
     1981 aacgtggggt tctgcgcgcc ctgctccggc gcggcgggcg cctgcccggc ggacgcgtcg
     2041 gcgccgcctg gtgggaggcc ctg
//
LOCUS       DAOSIF010000535         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_47314_length_2048_cov_3.002007, whole genome shotgun sequence.
ACCESSION   DAOSIF010000535 DAOSIF010000000
VERSION     DAOSIF010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47314_length_2048_cov_3.002007"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2048
                     /gene="dnaE"
                     /locus_tag="PLT35_13510"
     CDS             <1..>2048
                     /gene="dnaE"
                     /locus_tag="PLT35_13510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG30288.1"
                     /translation="YQSLCRLLTRAKLRAPKGEGALALGELEGCTDGLIAVIGREALA
                     AGRHGVGGLVDRLVGVFGRANVRLELQRHFQRDEELAGGMLRDLAAAFHVPLIASNGV
                     RFAEPSDRPLFDVLTCIRHKTTIERAGRRLTPNAERYLKPPAEMASLFADHPGALAET
                     RALADRIQYSMADLGYRFPDYPVPAGETAGSFLRAITLVGARERYRPFHARARAQIER
                     ELAIIEKLDLPGYFLIVWDIVNFCREQGILVQGRGSAANSAVCYSLGITAVDPVGMEL
                     LFERFLSEERGEWPDIDLDLPSGDRRERVIQHVYGKYGAHGAAMTANVITYRGRSASR
                     EVGKVMGLEPAQIDRLAGLLGRYGRGHFDGRDASGGSGDGSGGEAEGGDAQAMARDLR
                     TAGLDPDEPRVRLFVDLWRRIQDVPRHLGQHSGGMVLCRGRLDEVVPLENASRPGRVV
                     VQWDKEDCADMGIVKIDLLGLGMLAALQDAFTTINARRLPGTAPIEMWSIPPDDPQVY
                     DLLQRADTVGVFQVESRAQMATLPRLRPARFYDLVVEVALIRPGPITGQMVHPYLDRR
                     AGRAEVVYPHPSLEPILKRTLGVPLFQEQLLRMAMTAAGFTGGQAEELRRAMGFKRSE
                     RRMRAIEEQLREGMARNGITGEAADSVVRAITSFALYGFPESHSASFALIVYASAYLK
                     "
BASE COUNT          278 a          714 c          768 g          288 t
ORIGIN      
        1 ctaccagagc ctctgccgcc tgctcacgcg cgcgaagctg cgcgcgccga aaggggaggg
       61 cgcgctggcg ctcggcgagc tcgagggctg caccgacggc ctcatcgccg tgatcggccg
      121 cgaagccctg gcggcgggcc gccacggcgt ggggggcctg gtcgatcggc tggtgggcgt
      181 gttcggccgc gcgaacgtgc gtctcgagct gcagcgccac tttcagcgcg acgaggagct
      241 ggcaggcggc atgctgcgcg atctcgcggc cgcgttccac gtgccgctca tcgcgtcgaa
      301 cggcgtccgt ttcgcggagc cgtcggatcg cccgctcttc gacgtgctca cctgcatccg
      361 ccacaagacg acgatcgagc gggccggccg gcggctgacg ccgaacgccg agcggtacct
      421 gaagccgccg gcggagatgg cgagcctctt cgcggatcac cccggcgcgc tggcggagac
      481 gcgcgcgctc gcggatcgca tccagtactc gatggcggac ctcggctacc gctttcccga
      541 ctaccccgtg ccggccggcg agaccgccgg ctcgttcctg cgcgcgatca cgctcgtcgg
      601 cgcgcgcgag cgctaccggc cgttccacgc ccgcgcgcgc gcccagatcg agcgcgagct
      661 ggccatcatc gagaagctcg atctgcccgg ctacttcctc atcgtctggg acatcgtgaa
      721 cttctgccgg gagcagggga ttctcgtgca gggccgcggg tcggcggcca acagcgccgt
      781 gtgctacagc ctcgggatca cggcggtgga cccggtcggc atggagctgc tcttcgagcg
      841 gttcctgtcg gaggagcgcg gcgagtggcc cgacatcgac ctggatctgc cgagcggaga
      901 ccgccgcgag cgcgtcatcc agcatgtcta cgggaagtac ggcgcgcacg gcgcggcgat
      961 gacggccaac gtgatcacgt accgcggccg cagcgcgtcg cgcgaggtgg ggaaggtgat
     1021 gggcctggag ccggcgcaga tcgaccggct cgcggggctc ctgggccgct atggccgcgg
     1081 ccacttcgat ggccgcgacg cgagcggcgg ctccggggac ggcagcgggg gggaggccga
     1141 gggcggggac gcgcaggcga tggcgcgcga tctgcgcacg gccggcctcg atcccgacga
     1201 gccgcgcgtc cgtctgttcg tggatctgtg gcggcgcatc caggacgtgc cgcgccacct
     1261 cgggcagcac tcgggcggca tggtgctgtg ccgcgggcgc ttggacgagg tggtgccgct
     1321 ggagaacgcg agcaggccgg gccgcgtggt ggtgcagtgg gacaaggagg actgcgccga
     1381 catggggatc gtgaagatcg acctgctcgg cctcggcatg ctggccgcgc tgcaggacgc
     1441 cttcacgacg atcaacgcgc ggcgcctccc cggcacggcg ccgatcgaga tgtggagcat
     1501 cccgccggac gacccgcagg tgtacgacct gctgcagcgc gccgacacgg tgggcgtgtt
     1561 ccaggtggag tcgcgcgccc agatggcgac actgccccgg ctccggcccg cccgcttcta
     1621 cgatctggtc gtggaagtgg cgctcatccg gcccggcccc atcaccggcc agatggtgca
     1681 cccgtacctc gaccggcggg ccggccgcgc ggaggtggtc tatccccacc cgtcgctcga
     1741 gccgatcctg aagcgcacgc tcggcgtgcc gctcttccag gagcagctgc tgcgcatggc
     1801 catgacggcg gcgggcttca ccggcggcca ggcggaggag ctgcgccgcg ccatgggctt
     1861 caagcgatcg gagcggcgca tgcgcgccat cgaggagcag ctgcgcgagg ggatggcgcg
     1921 caacgggatc accggcgaag cggccgactc ggtggtccgg gcgatcacct cgttcgcgct
     1981 ctacggcttt cccgagtcgc actcggcgag cttcgcgctc atcgtctatg ccagcgcgta
     2041 cctcaagg
//
LOCUS       DAOSIF010000536         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_47348_length_2047_cov_3.995482, whole genome shotgun sequence.
ACCESSION   DAOSIF010000536 DAOSIF010000000
VERSION     DAOSIF010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47348_length_2047_cov_3.995482"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PLT35_13515"
     CDS             <1..1282
                     /locus_tag="PLT35_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG30289.1"
                     /translation="IAAAAPDAHTVALTFPAPFGPGLRLLDSLPIVPRHKLEAALGAG
                     TLREQWGPAAPPAQIAGLGPFVLQEYRAGERLVLARNPRYWRADASGTRLPYLDRLTL
                     EITPDQNAELLRLQSGQADLTQSEVRPDDYAALRRAAAAGTLRLADAGLAFDADAFWI
                     NLRPGAKAHQGRPWLARVELRRAISHAVNRRAFADEVFLGAAEPLWGPVPPSNAAWYA
                     PEGAIERQAYDPARAQALLAGLGLRDANRDGMLEDASGAPARFALVTQKGNTALEKGA
                     AFLRDELRKVGLGVDVVPLEVGSLVDRMTKGDYDALYFRFLTSDTDPAMNLDFWLSSG
                     GAHVWNMEQPSPATPWEQEIDTLVLKMAAERAPAERRRLFIQAQKVLADNLPVIYFAV
                     PRVFVATSTRVAGAVPAPVRPPVLWNADAIGASR"
     gene            complement(1433..>2047)
                     /locus_tag="PLT35_13520"
     CDS             complement(1433..>2047)
                     /locus_tag="PLT35_13520"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HOG30290.1"
                     /translation="TKGAAGVGAASGTPVTARTKLGSGQVVGLGRFLAEALVSPGASN
                     VRVFDPEAPAKKAFTGLKWFPPDPALQLTAAYAPNASPDKVIVMTSRGLMKEYYRAGT
                     LSFTVAGEPMRLTALATTPAPKPGDELFVPFRDATTGAETYEVGRYLTVRVERAGEAH
                     TLDFNLATNPLCNYSPHYNCPIPPKENVLPVAIRAGEMTYPSHR"
BASE COUNT          266 a          778 c          734 g          269 t
ORIGIN      
        1 catcgcggcc gccgcgccgg acgcgcacac ggtggcgctg acgttccccg cgcccttcgg
       61 tcccgggctg cgcctgctgg actcgctgcc gatcgtgccg aggcacaagc tcgaggccgc
      121 gctcggggcg ggcacgctgc gcgagcagtg ggggccggcc gcgccgcccg cgcagattgc
      181 gggcctgggg ccgttcgtgc tgcaggagta ccgggccggt gagcggctgg tcctcgcgcg
      241 caacccgcgc tactggcgcg cggacgcgtc cggaacgcgg ctcccgtacc tggaccgcct
      301 gacgctcgag attacgccgg accagaacgc cgaactgctg cgcctgcagt cgggccaggc
      361 ggacctgacg cagagcgagg tgcggccgga cgactacgcg gcgctccggc gcgcggcggc
      421 ggccgggacg ctgcgcctcg ccgacgcggg cctggcgttc gacgccgacg ccttctggat
      481 caacctgcgg ccgggcgcga aggcgcacca gggccgcccg tggctcgcgc gcgtcgagct
      541 tcggcgcgcg atctcgcacg ccgtgaaccg ccgcgcgttc gccgacgagg tcttcctggg
      601 cgccgccgag ccgctctggg gcccggtgcc gccgtcgaac gcggcctggt acgcgcccga
      661 aggcgccatc gagcgccagg cctacgaccc ggcgcgcgcg caggcgctgc tcgccggcct
      721 cggcctgcgc gacgcgaacc gcgacggcat gctggaagac gcctcgggcg cgcccgcgcg
      781 cttcgccctc gtcacgcaga aagggaacac cgccctcgag aagggcgccg cgttcctccg
      841 cgatgaactc aggaaggtcg gcctcggcgt cgacgtcgtg ccgctggaag tcggctcgct
      901 cgtggaccgc atgacgaagg gcgactacga cgcgctctac ttccgcttcc tcacgagcga
      961 caccgacccc gcgatgaacc tcgacttctg gctcagctcc ggcggcgccc acgtctggaa
     1021 catggagcag ccgtcccccg ccacgccgtg ggagcaggaa atcgacacgc tcgtgctgaa
     1081 gatggccgcc gagcgcgcgc ccgccgagcg ccgccggctc ttcatccagg cgcagaaggt
     1141 cctcgccgac aacctgcccg tgatctactt cgccgtgccg cgcgtcttcg tcgcgacgag
     1201 cacgcgcgtc gccggggccg tgcccgcgcc ggtccgcccg cccgtcctct ggaacgcgga
     1261 cgcgatcggg gcgtcacgct agcggcccgg acccccgtgg ccggggcccg ggagccaatc
     1321 cgacacaacg ccacgcgcga caccgccgcg cagtccgggc gccggaaccg ggagcccgcg
     1381 cggggcctgc ccgcgggccg cccggcgacg gccggggcca ggaccggcgc cgctagcggt
     1441 gcgacgggta cgtcatctcg ccggcccgga tggcgacggg cagcacgttc tccttcggcg
     1501 gaatcggaca gttgtagtgc ggcgagtagt tgcagagcgg gttcgtcgcc aggttgaagt
     1561 ccagcgtgtg cgcctcgccg gcccgctcga cccgtactgt caggtaccgg ccgacttcgt
     1621 acgtttccgc ccccgttgtg gcatctcgaa acggcacgaa caactcgtcg ccgggcttcg
     1681 gcgccggagt cgtggccagc gcggtgagtc tcatcggctc gcccgccacg gtgaacgaca
     1741 gcgtccccgc gcggtagtac tccttcatca gtccgcggct cgtcatgaca atgaccttgt
     1801 ccgggctggc gtttggagcg tacgcggccg tcagctggag cgcggggtcg gggggaaacc
     1861 acttcagccc ggtgaacgcc ttcttcgccg gcgcctccgg atcgaagacg cgcacgttgc
     1921 tcgcgcccgg cgacacgagc gcctcggcga ggaaccggcc gaggccgaca acctggccgc
     1981 tgccgagctt cgtcctggct gtcacgggcg tgcccgaggc cgcgccgacg cccgcggcgc
     2041 ccttcgt
//
LOCUS       DAOSIF010000537         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_47399_length_2046_cov_2.791060, whole genome shotgun sequence.
ACCESSION   DAOSIF010000537 DAOSIF010000000
VERSION     DAOSIF010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47399_length_2046_cov_2.791060"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLT35_13525"
     CDS             <1..738
                     /locus_tag="PLT35_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG30291.1"
                     /translation="QVPFSRIRQIEFERWSGLDASRPDWVWYDVKVTGLNPGEGYTGR
                     LDVRVMRGVAAGVPWYSYPATDATRGRLMYRIVFGDVTAPPTIPWEAQEPAAVPPPPA
                     VVVPQPAAPPAPPAPSEDDLFARLSIDDLNRQKPLDDVFFDFDKSNIRPDAEITLVRN
                     AAWLKRWPSVTIRVDGCADPRGTAEYNLGLGMRRADTVRAFLAAQGVSVDRVEVLSVG
                     ESQLVCTEQTEECWARNRAGRFHIIAK"
     gene            1005..1081
                     /locus_tag="PLT35_13530"
     tRNA            1005..1081
                     /locus_tag="PLT35_13530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1039..1041,aa:Arg,seq:cct)
     gene            1096..1833
                     /locus_tag="PLT35_13535"
     CDS             1096..1833
                     /locus_tag="PLT35_13535"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG30292.1"
                     /translation="MKRTQRRQLKVNEFAEWLLKTKAWFEANQKLVSYAGVGVAVAAV
                     ALLAFLWYRQMSAGRAATALAEAMSVAEAPIVAPPAPEPGKFPVQQPGTFPTERARLE
                     AALPKLLAVADGYPGSNAGVMAAYRAASALVALGRLPEAIDRYKQVAERSTGVQRTMA
                     RMGLAEAHVVAGQFDQAIAAFTELSAANADDVPGDGILMQLARAYKLSGRAEDAKKTF
                     KRVVVEFPQSPYVSAARREAEGEGAGS"
     gene            complement(1843..>2046)
                     /locus_tag="PLT35_13540"
     CDS             complement(1843..>2046)
                     /locus_tag="PLT35_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOG30293.1"
                     /translation="MPATGTPEPGGLSWRELLQLLRLVTARKTVVAADVVELCPIPGF
                     VAPNFLAARLVYKLIGYRFFPAR"
BASE COUNT          327 a          713 c          733 g          273 t
ORIGIN      
        1 caggtgccgt tcagccgcat ccggcaaatc gaattcgagc gctggagcgg tctggacgcg
       61 tcacggcccg actgggtgtg gtacgacgtc aaggtcacgg ggctgaaccc gggagagggc
      121 tacacgggca ggctggacgt ccgcgtcatg cggggcgtcg ccgctggcgt gccctggtac
      181 tcctacccgg cgaccgacgc gacccgcggc cggctgatgt accgcattgt gttcggcgac
      241 gtcaccgcgc cgccgacgat accctgggag gcccaggaac ccgcagcggt tccaccgccg
      301 ccagcggtcg tggttccgca gccggcagct ccgcccgcgc cgccggcgcc gagcgaggat
      361 gacctcttcg ccaggctgtc gatcgacgac ctgaacaggc agaaacccct cgacgacgtg
      421 ttcttcgact tcgacaagtc gaacatccgg cctgacgccg agatcacgct cgtccggaac
      481 gcagcctggc tcaagcgctg gccctcggtg accatccgcg tcgacggctg cgccgatccg
      541 cgaggcaccg ccgagtacaa cctgggcctc ggcatgcggc gcgccgacac ggtccgggcg
      601 ttcctggcgg cacaaggcgt gagcgtcgat cgcgtcgagg tcctctcggt gggggagagc
      661 cagctcgtct gcaccgagca gaccgaggag tgctgggccc gcaaccgcgc cggccggttc
      721 catatcatcg cgaagtagag cgccacccga ccggcgggaa ggaggacggg cggcatcccg
      781 cttcctccgg cgcatcgcag ggagatcgca cggcgaccgt ggtccgccat cgacggaggc
      841 gcgaagcgcc ggaccgccgg agaggcataa agggcggagc tgttcgcgtc ttcggccgca
      901 cgcgtcgcca cgcgttcgag ggccgcgccc ggcggctggc ctcggagcgg acccgatagg
      961 gagagacggc cggctcccgg gccgtgatag actcaggcat tcgggcccgt gtagctcaga
     1021 aggatagagc aacggtttcc taaaccgtag gccgcccgtt cgagtcgggc cacgggcacc
     1081 acacagggat cgagcatgaa gcgcacgcaa cgccgtcagc tcaaggtcaa cgaattcgcc
     1141 gagtggctgc tcaagacgaa ggcctggttc gaggccaacc agaagctggt ctcttacgcc
     1201 ggcgtcggcg tggccgtggc ggccgtcgcc ctcctcgcgt tcctctggta ccgccagatg
     1261 agtgccggcc gcgcggcaac ggcgctcgcc gaggcgatga gcgtggccga ggcgcccatc
     1321 gtcgcgccgc ccgctccgga gccggggaag ttccccgtgc agcagccggg cacgttcccg
     1381 accgagcgcg cgcggctcga ggcggccctg cccaagctgc ttgccgtcgc tgacggctac
     1441 cctggcagca atgccggcgt gatggcggcc taccgcgcgg cgtccgcgct cgtcgcgctg
     1501 ggacgcctgc cggaggcgat cgaccgctac aagcaggtag ccgagcgcag cacgggcgtc
     1561 cagcggacaa tggcccgcat ggggctggcc gaggcccacg tggtcgcggg ccagttcgac
     1621 caggccattg ccgccttcac ggaactgtcc gcggcgaacg ccgacgacgt gccgggcgac
     1681 ggcatcctga tgcagctcgc ccgcgcctac aagctgtcgg gcagggcaga ggatgcgaag
     1741 aagacgttca agcgcgtcgt ggtcgagttc ccgcagtcgc cgtacgtgtc ggcggcccgc
     1801 cgcgaggccg agggtgaggg agcgggcagc taggctccgc cctcagcgcg ccgggaagaa
     1861 ccggtagccg atcagcttgt agaccagcct cgccgcgagg aaattcggcg cgacgaagcc
     1921 cgggatgggg cagagctcga cgacgtcggc cgcgaccacg gtcttccgtg ccgtgaccag
     1981 cctcaggagc tgcagcagtt cgcgccagct caggccgccc ggctcgggtg tgcccgtcgc
     2041 cggcat
//
LOCUS       DAOSIF010000538         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_47425_length_2045_cov_5.891960, whole genome shotgun sequence.
ACCESSION   DAOSIF010000538 DAOSIF010000000
VERSION     DAOSIF010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47425_length_2045_cov_5.891960"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>238)
                     /locus_tag="PLT35_13545"
     CDS             complement(<1..>238)
                     /locus_tag="PLT35_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30294.1"
                     /translation="HWHACTRSGCAEASGRAAHSGGTATCTEQAVCEVCGKPYGAALG
                     HDFEAVWSGDGSEHWHACTRSGCAEASGRAAHSGG"
     assembly_gap    239..338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<339..1803)
                     /locus_tag="PLT35_13550"
     CDS             complement(<339..1803)
                     /locus_tag="PLT35_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30295.1"
                     /translation="MKKLMVAALVFLSILTAATGLFALTSPVASVNGTAYDSLQEAVD
                     AAARGQTQDERAVLLLADVDLGGGSVVIDAGDVTLDLNGHTVTGTAQALIVLEDGARL
                     AVTDEGSGCGAILCALEGAGCAIRLEGGSSLVVEAGTIDARGEDDEAVRCGAGSTVVV
                     NGGSVDGIARAKGAAEVRNEGEQNVLQYDLTIGDPPMAGAEVAGLSTIPGLSYPYGPA
                     FTGGEGVVHVWLPEGQTWITVQIGCAVYAGEIHGENPYTAKLSLTGYLHDFPQGWLAD
                     AADHWKSCRNCGEEAYRAQHAGGEATCTEPAVCPVCGVACGAPLGHDFEAEFTIDIEP
                     TCTEPGSKSRHCSRCDEVTDVTPIDPTGHRFSKDWSANDNGHWRDAICGHDLIDEGGP
                     HNPEPDDGDCTTEIRCAECGFVTTPAKEHLWSAWYTSNTEHWHVCEHINCAREADRAQ
                     HGGGTATCTEQAVCEVCGKLYGAALGHDFEEIWSGDGS"
BASE COUNT          298 a          695 c          631 g          321 t
ORIGIN      
        1 ccgccggaat gcgcggcgcg cccgctcgcc tcggcgcagc cggagcgcgt gcaggcatgc
       61 caatgctcgc ttccgtcgcc cgaccagacc gcctcgaagt cgtgcccgag agccgcgccg
      121 taaggtttac cgcagacctc gcagaccgcc tgttcggtgc aggtggccgt gccgccggaa
      181 tgtgcggcgc gcccgctcgc ctcggcgcag ccggagcgcg tgcaggcatg ccaatgctnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cttccgtcgc ccgaccagat
      361 ctcctcgaag tcgtgcccga gagccgcgcc gtagagtttg ccgcagacct cgcagacagc
      421 ctgttcggtg caggtggccg tgccgccgcc gtgctgcgcg cggtcggctt cccgcgcgca
      481 gttgatgtgc tcgcaaacat gccagtgctc cgtgttgctc gtataccacg cgctccacag
      541 gtgttccttc gccggggtcg tgacaaagcc gcactccgcg cagcggatct ccgtcgtgca
      601 gtcgccgtcg tccggttcgg gattgtgcgg accgccctcg tcgatcagat catgcccgca
      661 gatcgcgtcg cgccagtggc cgttgtcgtt tgccgaccaa tccttcgaaa acctgtgccc
      721 ggtcgggtcg atgggcgtca cgtcggtcac ctcgtcgcag cgcgagcagt ggcgcgactt
      781 gctgccgggc tccgtgcagg tgggttctat gtctatggtg aattcggcct cgaagtcgtg
      841 tccgagcgga gcaccgcagg cgacgccgca aaccgggcac acggcaggtt cggtgcatgt
      901 ggcctcgccg ccggcatgct gcgcgcggta tgcctcctcc ccgcagttcc ggcacgattt
      961 ccaatgatcg gcggcgtccg ccagccatcc ctgcgggaag tcgtgcaggt atccggtcag
     1021 gctcagcttc gccgtatacg ggttttcgcc gtggatttca ccggcataaa ccgcgcagcc
     1081 gatctgcacg gtgatccagg tctgcccctc gggaagccag acgtggacca cgccctcgcc
     1141 gccggtgaac gccggaccgt acgggtacga cagccccggt atcgtcgaaa ggccggccac
     1201 ctcggcgccc gccatgggcg gatcgccgat cgtcaggtcg tactgcaaga cattctgttc
     1261 gccttcgttg cgcacctccg cagcgccttt ggcgcgcgcg atcccatcca cgctgccgcc
     1321 gttgaccacg accgtggaac cggcgccgca gcgaaccgcc tcgtcgtcct cgccgcgcgc
     1381 gtcgatcgtg ccggcttcca ccacgaggct gctcccgcct tcgagccgga tcgcgcagcc
     1441 cgcgccctca agcgcgcaga ggatcgcgcc gcagcccgat ccctcgtccg tgacggccag
     1501 ccgcgcgccg tcctcgagca cgatcagcgc ttgcgccgtg ccggtgacgg tatgtccgtt
     1561 caggtccagc gtcacgtctc cggcgtcgat gacgacggag ccgccgccga gatccacatc
     1621 cgcgagcagc agcacggcgc gctcgtcctg cgtttggccg cgcgccgcgg cgtccacggc
     1681 ttcctgaagg gaatcatagg ccgtcccgtt gaccgaggcg acgggcgacg tcagggcgaa
     1741 aaggcccgtc gcggccgtca ggatcgacag gaataccaac gcggcaacca tcagcttttt
     1801 catgacgctc ctccacatcc ggcggtatgg cgatatgcgc gcacaaaggc gcatacgccc
     1861 attttttcta ttataaggaa aagcgccgcc ccgcgcgggg cggctccctg aaacggccgg
     1921 gattcgccat gaagcgcacg ctctgccgca agacagccgc acgggataga ggaatggaac
     1981 gcgaacggac gggggaaggg cggcttaacg cgcgggaaaa cgcgaaggcg cgctcgttcc
     2041 cgcgg
//
LOCUS       DAOSIF010000539         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_47538_length_2041_cov_3.854985, whole genome shotgun sequence.
ACCESSION   DAOSIF010000539 DAOSIF010000000
VERSION     DAOSIF010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47538_length_2041_cov_3.854985"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="PLT35_13555"
     CDS             complement(<1..1468)
                     /locus_tag="PLT35_13555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HOG30296.1"
                     /translation="MPLVYGAFYTWANESPQTRLDQVTAAVVNLDEPVTLKTADGKDQ
                     IVPLGRSVVGKLTSSDSSSNFTWVLTDAPAAAAGLAEGRYAAVLTIPADFSRSATSLN
                     GPAADVRVARLRLETNDAVNYLDGALGRAIATATSAETSRTITEGYLTALYAGFGTLG
                     DKLGSAADGAGELASGATSLSDGASKAASGAGQLADGTAKVANGADALAAGAGKLATG
                     AGQAADGARQLQAGTAPLVAGARELAAGATKLSSGAATFAAGIDPLVAGSSQAAGGAT
                     SLQAGVTAYTQGIDGLAAGCLTSGAAPAYCAQLAQVAGGGAGLRSGAAGVAQGTSHVA
                     GGVAQLRTGATQLGTSAATFGAGVSSLGAGIEKLGAGVGQLAGNLPALARGASDLAAG
                     ARSLASGAEQAASGGSQLKNGVDALASGSAKLGTGATELSAGLGQAVANIPSYSQAER
                     AAVAAVAAAPVTAEASRINAVKNNGAGMAPYFMGLALWV"
     gene            complement(1558..>2041)
                     /locus_tag="PLT35_13560"
     CDS             complement(1558..>2041)
                     /locus_tag="PLT35_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30297.1"
                     /translation="LLSYAVSTEPEAHCASSLAADRAVRSLQPLERWVLGVCLALIGD
                     PELVVCDDVDGLRGEVDRRAAWAVVGRLTQLAAAPGRSRRGTGEHPLAVVASCRDPHL
                     AREALGGIPVVDVPMQRPAGRVGVAGDDLAGPRPESLLPPDADPLADPMTDLFTSKVR
                     "
BASE COUNT          278 a          763 c          739 g          261 t
ORIGIN      
        1 ccacccacaa ggccagaccc atgaagtagg gcgccatgcc ggcgccgttg ttcttcacgg
       61 cgttgatccg cgacgcctca gccgtcacgg gtgcggcggc caccgccgcc acggcggctc
      121 gctcggcctg cgaatagctc gggatattgg ccacggcttg gcccagcccg gcgctgagtt
      181 cggttgcgcc cgtgccgagt ttggcgctgc cggacgcgag cgcatcgacg ccgttcttga
      241 gctgcgatcc accgctcgcg gcttgctcgg cgccggacgc cagcgaccga gcgccggcgg
      301 caaggtccga ggcgccgcgg gcgagcgctg gcaagttgcc ggcaagctga cccacgcccg
      361 cgcccagctt ctcgatccca gcgccgagcg agctcacgcc cgcaccaaac gtcgccgcgc
      421 tggtcccgag ttgcgttgca cccgtgcgca attgcgcgac gcctcccgcg acgtgggacg
      481 tgccctgcgc gacccctgca gccccgctgc ggagacccgc cccacctcca gcgacttgcg
      541 cgagttgggc gcagtatgcc ggtgcggcgc cactcgtgag gcagcccgcg gccagcccgt
      601 cgatcccttg cgtgtatgcc gtgacgcccg cctgaaggct ggtcgcccca ccggcggcct
      661 gcgacgaccc ggccacgagc gggtcgatgc cggccgcgaa cgtcgcagca ccggatgaca
      721 acttcgtcgc gccagcagcc aactcgcgag cgccagccac gagcggcgcc gtcccggcct
      781 ggagctgccg agcaccgtcc gccgcctgtc cggcgccggt cgcgagcttg cccgccccag
      841 cggccagggc atcggccccg ttggccacct tcgccgtgcc gtcggcgagt tgaccggccc
      901 cgctcgccgc cttgctcgcg ccgtccgaca gcgatgtcgc tcccgaggcc agctccccgg
      961 cgccgtcggc cgcggagccg agcttgtccc cgagcgtccc gaacccggca tacagggccg
     1021 tgagataacc ctcggtgatc gtcctggacg tctccgcgct cgtcgcggtc gcgatggccc
     1081 gcccgagcgc cccgtcgagg tagttgaccg catcgtttgt ttcgagccgc agccgcgcca
     1141 ctcgcacgtc ggccgccggc ccgttgagcg aggtcgccga gcgcgagaag tcggcgggga
     1201 tcgtgaggac cgcggcatac cggccctcgg cgaggccggc agcggcggcg ggcgcgtcgg
     1261 tgagcaccca cgtgaagttc gacgacgagt ccgaactcgt gagcttgccg acgaccgacc
     1321 ggccgagcgg cacgatctgg tccttgccgt cggcggtctt gagcgtgacg ggttcgtcga
     1381 ggttgacgac cgcggccgtc acctggtcga gccgcgtctg ggggctctcg ttggcccagg
     1441 tgtagaacgc gccgtagacg agcggcacaa ggatcgcggc gatgagcgcg atgaacgccc
     1501 gcgggctccc cgtgaagcgg cggagttcgg cggcgggcag cgtgggtcgg cgagacatca
     1561 gcgcaccttc gaggtgaaga ggtcggtcat cggatcggcc agcgggtcgg catccggtgg
     1621 caagagtgac tcgggacgtg gcccggcaag gtcgtcgccc gccaccccca cgcgaccggc
     1681 aggccgctgc atggggacgt cgacgacggg tatgccgccg agcgcctccc gcgcgaggtg
     1741 cgggtcgcgg cacgaggcga cgacagccag cgggtgctcg ccggtgccgc gccgcgagcg
     1801 cccgggcgcc gccgcgagct gggtgagccg cccgacgacg gcccaggcgg cccgccgatc
     1861 gacctcgccg cgcaacccgt cgacgtcgtc gcacacgacg agctcggggt ctccgatcag
     1921 ggcgaggcaa accccgagga cccaccgctc gagcggctgg agcgaccgca cggcccggtc
     1981 ggcggcgagc gacgacgcgc agtgggcctc gggctcggtc gacacggcat acgagaggag
     2041 g
//
LOCUS       DAOSIF010000540         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_47615_length_2038_cov_3.167423, whole genome shotgun sequence.
ACCESSION   DAOSIF010000540 DAOSIF010000000
VERSION     DAOSIF010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47615_length_2038_cov_3.167423"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="PLT35_13565"
     CDS             complement(<1..1626)
                     /locus_tag="PLT35_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30298.1"
                     /translation="MPSPRVSEFASRLDGRLAVWPGTMAACVDERLIEMDRTRMVEGL
                     YAKRTALWSDDPAVQATIAGRLGWLDAPDWILPLVPRLERFAEDVRARGTTHVVLLGM
                     GGSSLAPEVIRAVMGVRPGWPAFRMLDSTDPASVAAVDAAIDLPRTLFLLASKSGGTI
                     EPNSMAAHFRGRLEALGLARWSDHFVAITDEGTALHRRAVAEGFRDLFLNPSDIGGRY
                     SALSFFGLVPAALMGHSPAAIVDWARAMLWLCGPHRPLTANPAARLAAVMAAGALSGR
                     DKLTLVAPDDLSAIGLWADQLVAESTGKQGRGLIPVVGERLGAPRAYGSDRVMVHLGH
                     ERRPDAAARDRLREIAADGTPLVQIELVEPAAVGAEFVRWELATAMAGALVGVNPFDE
                     PNVQQAKDATGRLLDAHRAGQPLTHPARAAVIAGVSARLSKAACDGLGNRPADAFLSL
                     LRTGDYFAILAYLGPDPQWLEPLERLREAVRAETRCATTCGYGPRYLHSTGQLHKGGP
                     NTGVYLVITAEPAADITVPGEAFSFGVLERAQAV"
     gene            1792..>2038
                     /locus_tag="PLT35_13570"
     CDS             1792..>2038
                     /locus_tag="PLT35_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30299.1"
                     /translation="MSESYRDALVRKKAEILAAGVRPLAAAMEGSSRQGDLADQADGN
                     NEVHIQLKLKQTDAKILQAIEEALVRLDKGTYGTCRDC"
BASE COUNT          311 a          715 c          750 g          262 t
ORIGIN      
        1 gaccgcctgg gctcgctcga ggacgccgaa actgaacgcc tcgccgggta ccgtgatgtc
       61 agcggcgggc tcggccgtga tgacgagata gactcccgtg ttgggcccgc ccttgtgcag
      121 ctgcccggtt gaatggaggt aacgcggtcc gtacccgcac gtcgtcgcgc atctggtctc
      181 ggcccgaacc gcctcccgga gccgctcgag cggctcgagc cactgcgggt cggggcccag
      241 gtaggccagg atggcgaagt agtcccccgt ccgcagcagc gacaggaagg cgtcggccgg
      301 ccggttcccg agcccgtcgc aggcggcttt cgacagccgg gcgctcaccc cggcgatcac
      361 cgccgcccgg gccgggtgcg tcaggggctg ccccgcccgg tgcgcgtcca gcaggcgtcc
      421 cgtggcgtcc ttcgcctgct gcacgttggg ctcgtcgaac ggattcacgc ccacaagcgc
      481 gcccgccatc gccgtggcga gctcccagcg cacgaattcc gcgccgacgg ccgccggctc
      541 gaccagctcg atttgcacga gcggcgtacc gtccgcggcg atctcgcgca accggtcgcg
      601 cgcggccgcg tccggccggc gctcgtggcc gaggtgaacc atcacgcggt cggacccgta
      661 ggcgcgtgga gctccgagcc gctccccgac aaccgggatg aggccgcgcc cctgcttccc
      721 tgtgctctcc gcgaccagtt ggtccgccca gaggccgatg gcggagaggt cgtcgggcgc
      781 caccagcgtc agcttgtccc ggccgctcag cgcgccggcg gccatcacgg cggcgagccg
      841 cgcggccggg ttggccgtca gcggccggtg cggcccgcac agccagagca ttgcgcgagc
      901 ccaatcgacg atggcggcag gggagtggcc catcagcgcg gccggcacga gcccgaagaa
      961 cgacagcgcg gaataccgcc cgccgatgtc cgaagggttc aggaagaggt cccggaaacc
     1021 ctcggcgacg gcgcggcggt gcagggccgt gccttcgtcg gtgatggcga cgaaatggtc
     1081 ggaccagcgc gcgaggccga gggcctcgag gcgtccgcgg aagtgcgccg ccatcgagtt
     1141 tggctcgatc gtgccgccgg acttgctcgc gagcaggaag agcgtgcgag gcagatcgat
     1201 ggcggcgtcg acggccgcga cgctcgccgg gtccgtcgaa tcgagcatcc gaaacgccgg
     1261 ccagccgggc cgcacaccca tcacggcgcg aatgacctcc ggcgcgaggc tggacccgcc
     1321 catgccgagc agcaccacgt gcgtcgttcc cctggcgcgg acatcctcgg cgaaccgctc
     1381 cagccgcggc acgaggggga ggatccagtc cggggcgtcg agccagccga ggcggcccgc
     1441 gatggtcgcc tgcacggccg gatcgtcgga ccacaaggcg gtccgcttgg cgtaaaggcc
     1501 ctcgaccatc cgggtgcggt ccatctcgat gaggcgctcg tccacgcagg cggccatcgt
     1561 gccgggccag acggcgagac ggccatcgag gcggctggcg aactcggaca cgcgcggtga
     1621 cggcatggag tcctgggaag tagccatgtc tcataggata gcggagccgg gccgcgcgat
     1681 cacctgctcg cccgcgcggc cgctgcgccg gcgtccgccc cgggcgcagt aagcgtggta
     1741 tcatggctgt accggttggt tcctgtggct gacggagacg gagtgggagt gatgtccgag
     1801 agctaccgcg acgcgctcgt gcgcaagaag gccgaaatcc tggcggccgg cgtccgtccg
     1861 ctggcggcgg ccatggaggg cagttcgcgc cagggcgatc tggccgacca ggcggacggc
     1921 aacaacgaag ttcacatcca gctcaagctg aagcagacgg acgcgaagat cctgcaggcg
     1981 atcgaagagg cgctcgtgcg gctcgacaag ggcacctacg ggacgtgccg ggactgcg
//
LOCUS       DAOSIF010000541         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_47774_length_2033_cov_3.960566, whole genome shotgun sequence.
ACCESSION   DAOSIF010000541 DAOSIF010000000
VERSION     DAOSIF010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47774_length_2033_cov_3.960566"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLT35_13575"
     CDS             complement(<1..672)
                     /locus_tag="PLT35_13575"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG30300.1"
                     /translation="MLSLAGFIIIVIVLVALLSGRVSPIVPLVIVPIAGALAAGFSPA
                     QVAGYTNEGLHAVMSVATMFIFAVLFFSLMNDAGLFDPLVKKLVGITKGNVVWVCVAT
                     VLLGAVGHLDGVGATTFLILIPPLLPVYKRLRMDPYLLLLLGSTAAAVMNMVPWGGPV
                     GRAAVVLTKDPTALWRPLIPLQLIGLALLCVMAVILGMRERRRIARRQAAEASGPDEA
                     VGMPPS"
     gene            1039..>2033
                     /locus_tag="PLT35_13580"
     CDS             1039..>2033
                     /locus_tag="PLT35_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG30301.1"
                     /translation="MKSLVTGALVAGLLLCPSTPGNAQTGRPAVEWVVPALEPNVPQT
                     PEEVAAGRQHGRTLPPPELLQPTIDPALPSYAPRPGRLNGTFRGGCSDVLTGLVRQWF
                     EKFRQYQPDVTLTISPPYAGSAGAVELAKENLDFVFVSRELRPDDIRDFRAHFGYDPL
                     SVPVSGGSYRHYGALDAIAVFVHPDNPITALSLTQLDAMFSSTRHRGGQAITTWGQLG
                     LTGDWADKPIRLYGVRPWNGIEEFVRQRVLSVGERRGEWREDINFEKLVFGLAARVAA
                     DRYAVGYGGVAFIDQAVRVLPLIVREGEAPRAPTYDNVARADYPLTRLVYFNTNKA"
BASE COUNT          346 a          700 c          676 g          311 t
ORIGIN      
        1 cgagggcggc atgccgaccg cctcgtctgg gcccgacgcc tcagccgcct gcctgcgggc
       61 gatccgccgc cgctcgcgca tgccgagtat gacggccatg acacacagca gcgccagccc
      121 aatcagctgc agcgggatca gcggtcgcca gagcgcggtc gggtccttcg tcaggaccac
      181 ggcggcccgg ccgacgggcc cgccccacgg caccatgttc atcacggccg cggcggtgct
      241 gccgagcaac agaagcaggt agggatccat gcgcagccgc ttgtacacgg ggagcagcgg
      301 cgggatcagg atcaggaacg tcgtcgcacc cacgccgtca aggtggccga cggccccgag
      361 gagcaccgtc gccacgcaca cccacacgac gttgcccttc gtgatcccca ccagcttctt
      421 gaccagcggg tcaaagagcc ccgcgtcgtt catcaggctg aagaacagga ccgcgaagat
      481 gaacatggtg gccacactca tgacggcatg gaggccctcg tttgtgtagc ctgcgacctg
      541 cgcggggctg aagccggcgg cgagcgcgcc ggcgatcggg acgatgacga gcggcactat
      601 cggagagacc cggccgctca gcagcgccac caggacgatc acgatgatga tgaatccagc
      661 gaggctaagc atatcgctcc agtgaggcgc gccggccttc tcgggctgcc tgctgcatgc
      721 ggcagccgcc ccggggacgg gactcaccag ggaatatgta gtccgccagc cgagcggcca
      781 caactgaact cttcctgaag tcgcgttaac tcgcgccgcg tcccgcggca gccggccgga
      841 ggctgcgggg gccccgagac aggggccgcc tgggagcacg cgcctcccgg gcctgtcggc
      901 ccgtcctcca ggcgtgatcc tcgccgccgc ctggacgccg gcccctgccg gaattcagaa
      961 taacttcagg ttgtgcgggc ggctgattgt gccataaagg cgtgcatgcg aagcctcacc
     1021 gaaaggagcc gcccgtccat gaaatccctg gttaccggcg cactcgtggc aggtctgctt
     1081 ctgtgccctt caacgcccgg caacgcccag accgggcggc ccgccgtcga gtgggtcgtc
     1141 cccgcactgg agcccaacgt cccccagaca ccggaggaag tcgcggcagg ccgacagcac
     1201 ggtcgcacgt tgccgccgcc cgagttgctt cagccgacca tcgaccccgc gctcccgtcg
     1261 tacgcaccgc gtccgggcag gctgaacggc acgttcaggg gcgggtgctc cgacgtattg
     1321 accgggctgg ttcgacagtg gttcgagaag ttccggcagt atcagcctga tgtcacgctc
     1381 acgatctcgc ctccgtacgc cggcagcgcc ggagccgtcg agctcgccaa agagaacctg
     1441 gatttcgtct tcgtgtcgag ggagctgagg cccgacgaca tcagggactt ccgggcgcac
     1501 ttcggctacg atccgctcag cgttccggtg tcgggtggtt cttaccggca ttacggagcc
     1561 ctcgacgcca tcgctgtctt cgtgcatccg gacaatccga tcactgcgct cagcctcacg
     1621 cagctggatg cgatgttctc ctccacccgg caccgaggcg gccaggcaat caccacctgg
     1681 gggcagctcg ggctgactgg ggactgggcg gacaagccaa tcaggctgta cggcgtcagg
     1741 ccctggaacg gcatcgaaga gttcgtgcgt cagcgcgtcc tgagcgtcgg agagcggcgg
     1801 ggtgaatggc gggaggacat caacttcgag aagctggtgt tcggcctggc cgcccgcgtg
     1861 gccgctgatc ggtacgccgt cggctacggc ggcgtcgcgt tcatcgatca ggccgtccgc
     1921 gtgctgcctt tgattgtgcg ggagggggag gccccgcgtg caccgacgta cgacaacgtc
     1981 gcgcgggccg actatcctct gacccgcctg gtgtacttca ataccaacaa ggc
//
LOCUS       DAOSIF010000542         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_48065_length_2024_cov_2.751651, whole genome shotgun sequence.
ACCESSION   DAOSIF010000542 DAOSIF010000000
VERSION     DAOSIF010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48065_length_2024_cov_2.751651"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLT35_13585"
     CDS             <1..186
                     /locus_tag="PLT35_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30302.1"
                     /translation="HTLEEIRKYIEADSLRFLSLEGLLDAVNSHRDDYCTSCYTGRYP
                     LPFPRHEAEQVRGEGRT"
     gene            432..1457
                     /gene="purM"
                     /locus_tag="PLT35_13590"
     CDS             432..1457
                     /gene="purM"
                     /locus_tag="PLT35_13590"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOG30303.1"
                     /translation="MDYKTSGVDIDAGNEAVRRIKGLAARTFTPNVLSSIGSFGGLFR
                     LGEGYRDPVLVASADGVGTKLKVAFMTGRHDTVGQCLVNHCVNDILVQGATPLFFLDY
                     LAAGTLSPDVAAQVVEGVSAACLENGCALIGGETAEMPGFYAPGEYDMAGFIVGVVER
                     DRLITGERLRPGDALLGLRSTGLHTNGYSLARAIVFEKLGLQADSPVEELGCTIGEEL
                     LRVHRSYLPAVRPLLEAGLVKGLAHITGGGITENLPRILPPGTAALVDRSAWTPNAVF
                     RYLRRVGGVPDDDMYRTFNMGIGMIVGCAQDDAERVTVMLQAAGETRVHRVGRIIEGE
                     PGVVYGS"
     gene            1602..>2024
                     /locus_tag="PLT35_13595"
     CDS             1602..>2024
                     /locus_tag="PLT35_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG30304.1"
                     /translation="MRRRERWEYLGSAPRVAWDAIVGGQYRFTFDTMPMAVRGMSWPA
                     RLNLLAAGLNLARRRLRPWSWPLHMQIELTSFCDLECPVCPTGLGALRRAASAIDPEL
                     LEQILREAGPYLLTLSLWAWGEPLLYKHLGRALEAAGRY"
BASE COUNT          298 a          711 c          730 g          285 t
ORIGIN      
        1 cacaccctcg aggagatccg gaagtacatc gaggccgaca gcctgcggtt cctcagcctc
       61 gaagggctgc tcgacgcggt gaactcgcac cgcgacgact actgcacgtc gtgctacacg
      121 ggacgatatc ccttgccatt cccgcgccac gaggcggaac aggtgcgggg ggaagggagg
      181 acgtaaagga ggcggaggag ctggaagtca gcctgcccgc cgaagctggc cggcaggcag
      241 tctggcgaag gcgagaatcc agagttcaga aggcggaagc cagaatgcag acccggcgta
      301 cgctgcgccc gcgaacgagc gcccggcgcc gagcccccag cgcctggcgt ccagccccga
      361 atcccgagtc ccgagtacag gcctagaatg gagcccgggg ccttcgggcc ccggggatca
      421 tgagtggagc gatggactac aagacatccg gcgtcgacat cgatgccggc aacgaggccg
      481 tgaggcggat caaggggctg gcggcccgca cgttcacgcc gaacgtgctc tcgtcgatcg
      541 ggtcgttcgg cgggctcttc cgcctcggcg agggctaccg cgaccccgtg ctcgtcgcga
      601 gcgccgacgg tgtcggcacg aagctcaagg tcgcgttcat gaccggccgc cacgacacgg
      661 tcgggcagtg cctggtcaac cactgcgtga acgacatcct ggtccagggg gcgacgccgc
      721 tcttcttcct cgactatctc gccgccggca cgctgtcccc cgacgtggcc gcgcaggtcg
      781 tcgagggggt ctccgccgcg tgcctcgaga acggctgcgc cctgatcggc ggcgagacag
      841 ccgagatgcc cggcttctac gcccccggcg agtacgacat ggccggcttc atcgtgggcg
      901 ttgtcgagcg cgaccggctg atcacgggcg agcgcctgcg cccgggcgac gccctgctgg
      961 gcctgcgctc gacggggctg cacacgaacg gctactcgct cgcgcgcgcc atcgtgttcg
     1021 agaagctcgg gctgcaggcc gactcgccgg tcgaggaact gggctgcacg atcggcgagg
     1081 aactgctgcg cgtgcaccgg tcatacctgc cggccgtgcg cccgctccta gaggccggcc
     1141 tcgtgaaggg cctggcgcac atcacgggag gcggcatcac cgagaacctg ccgcgcatcc
     1201 tgccgcctgg cacggccgcg ctcgtcgaca ggtccgcgtg gacgccgaac gccgtcttcc
     1261 gctacctgcg gcgcgtcggc ggcgtgcccg acgacgacat gtaccgtacg ttcaacatgg
     1321 gcatcgggat gatcgtcggc tgcgcccagg acgatgcgga gcgcgtgacc gtgatgctgc
     1381 aggcagcggg cgagacgcgc gtccaccgcg tcggccgcat catcgagggc gagccgggcg
     1441 tcgtgtacgg ctcctgacag gctgtcgccc ggcggccatc gctcccgcgc gctggcctgc
     1501 cgtccttgcc ggatgtctgg gcgcgcggcc cgtgccggcg gcgggcgcgc cgcccggccc
     1561 ggcgccggag cgtctcgtgc gggcggaggt caccgggtat cgtgagacgg cgagaacgtt
     1621 gggagtacct cggcagcgcg ccaagggtcg cgtgggacgc catcgtcggc gggcaatacc
     1681 gcttcacctt cgacacgatg cccatggccg tccgcgggat gtcgtggccg gcgcggctga
     1741 acctgctcgc ggcggggctc aatctggcca ggcggcgcct gcgcccgtgg agctggccgc
     1801 tgcacatgca gatcgagctg accagcttct gcgacctcga gtgccccgtc tgcccgacgg
     1861 gcctcggcgc gctcaggcgc gccgccagcg ccatcgaccc ggagctgctc gagcagatcc
     1921 tgcgcgaggc cgggccctac ctgctgacgc tctcgctctg ggcgtggggc gagccgctgc
     1981 tctacaagca tctcggccgg gcgctcgagg cggctggccg ctac
//
LOCUS       DAOSIF010000543         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_48420_length_2012_cov_3.542156, whole genome shotgun sequence.
ACCESSION   DAOSIF010000543 DAOSIF010000000
VERSION     DAOSIF010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48420_length_2012_cov_3.542156"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..893
                     /locus_tag="PLT35_13600"
     CDS             243..893
                     /locus_tag="PLT35_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG30305.1"
                     /translation="MTVPASRARVRRPHAVEPGARERLLCAAVELFERKGYAAASVRD
                     IVEQAGVAKPALYYYFRNKEGILLAALELVASELDRTLDRAASAPGSARARLAGLCTL
                     LLVESRSHASLVRVAHAVFFSPRESLPPFDFRVFDRIVARHLRRIVGSGVARGEIRAE
                     ASRLITQAVQGLVMLAVGQHVGRRRGRLSPKDMRDLVDLVFDGAGAGPRPSKGASS"
     gene            890..2002
                     /locus_tag="PLT35_13605"
     CDS             890..2002
                     /locus_tag="PLT35_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG30306.1"
                     /translation="MSVRFVSWLFLPAAALAASACSGSSAPAPARPAQEAGRPPVAVT
                     VAPAAASAFEESVEIIGTLQPKFSADVKSEITGTVKAVYVTEWVPVRKGAVLAQLDTS
                     EAEAGVAALKAVEAQARVAEARARREYDRALQLKQYGLITPQNLDDAKTAVEAAEAAT
                     AAAVAQTRTGETRLAKARIVAPIDGVVALRGINVGDRVENMGGSAPIFRIVDTRLFDL
                     TASVPSSQAASVRVGQPIRFTTDSAPGRTFEGRVKFINPEVDAASRSTRVGVEVPNPD
                     GQLKGGAFVEGRIVVASRTDVLQVPREALMNWNVEEQRAQVFVARGGLAEKRDVKTGV
                     SGGATVEILSGLQAGDPVVIRGAWAPVRGSHHGRDG"
BASE COUNT          245 a          773 c          722 g          272 t
ORIGIN      
        1 tgcactcggt ggccgaggtg gtcctctacg cggtccggcg cggcatcatt tcctagcgcg
       61 gagcagtcgg cgcgggccgg gcctgccggg gccgcggaac cgcgcggccc gggtgttcgt
      121 ctgtctgggg agagcctcgg gtggaggcgc cgcccggccg cggcggaggc gcgcttgcgc
      181 ccggcgggcc gcgtgcggta ttctaccgac cggtaggtag ggatctgccc ctcgtcgcac
      241 gcatgaccgt cccggcatcc cgcgcacgcg tacgccgccc ccacgccgtc gagcccggcg
      301 cccgcgagcg cctcctctgc gccgccgtcg agctcttcga gcgcaagggg tacgccgccg
      361 cgtcggtgcg cgacatcgtc gagcaggccg gcgtcgccaa accggcgctc tactactact
      421 tccgcaacaa ggaaggcatc ctcctcgccg ccctcgagct ggtggcctcc gagctcgacc
      481 gcacgctcga ccgggccgcg tccgccccgg gcagcgcgcg cgcccgcctc gccggcctct
      541 gcacgctcct gctcgtcgag tcgcgcagcc atgcctccct ggtgcgcgtc gcgcacgcgg
      601 tcttcttcag cccccgcgag tccctgcccc cgttcgactt cagggtgttc gaccggatcg
      661 tggcccgcca cctccgccgc atcgtcggca gcggcgtcgc ccggggcgag atccgggccg
      721 aggcgtcgcg cctgatcacc caggcggtgc agggcctggt gatgctcgcc gtcggccagc
      781 acgtcggccg ccgccgcggg cggctgagcc cgaaggacat gcgggacctc gtcgatctgg
      841 tgtttgacgg cgcgggcgcg ggcccgcgcc cttcgaaggg tgcttcatca tgagcgtgcg
      901 attcgtctcc tggctgttcc ttcccgccgc cgccctcgcc gcgtcggcct gctccggctc
      961 gtcggcgccc gcgccggcgc ggcccgcgca ggaagccggc cgcccgccgg tggcggtcac
     1021 cgtcgcgccg gccgccgcgt cggcgttcga ggagtcggtc gagatcatcg gcactctgca
     1081 gccgaagttc tccgccgacg tcaagtccga gatcaccggc acggtcaagg ccgtctacgt
     1141 gaccgagtgg gtgccggtcc gcaagggcgc cgtcctcgcg cagctggaca ccagcgaggc
     1201 ggaggccggc gtcgccgccc tcaaggccgt cgaggcgcag gcccgggtgg ccgaggcccg
     1261 cgcgcgccgc gagtacgatc gcgcgctcca actcaagcag tacggcctca tcacgcccca
     1321 gaacctcgac gacgcgaaga ccgccgtcga ggcggccgag gccgccacgg ccgccgccgt
     1381 cgcccaaacc cgcaccggcg aaacgcgcct cgccaaggcc cgcatcgtcg cccccatcga
     1441 cggcgtcgtc gccctgcgcg gcatcaacgt cggcgaccgg gtcgagaaca tgggcggcag
     1501 cgcccccatc ttccgcatcg tggacacgcg gctcttcgac ctgaccgcgt ccgtgccctc
     1561 ctcgcaggcc gcgtcggtgc gcgtcggcca gcccatccgc ttcacgaccg actcggcgcc
     1621 gggccgcacg ttcgagggca gggtcaagtt catcaacccc gaggtcgacg ccgcgagccg
     1681 gtcgacccgg gtgggagtcg aggtgccgaa tccggacggc cagctcaagg gcggcgcctt
     1741 cgtcgagggc cgcatcgtgg tggccagccg gaccgacgtg ctccaggtgc cgcgcgaggc
     1801 cctgatgaac tggaacgtcg aggagcagcg ggcccaggtc ttcgtcgccc gcggcggcct
     1861 ggccgagaag cgcgacgtga agaccggggt gtccggcggg gccaccgtcg agatcctgtc
     1921 ggggctccag gccggcgacc cggtggtgat ccgcggggcc tgggctccag tccggggatc
     1981 gcatcacggt cgcgacgggt gaaggggcgt ag
//
LOCUS       DAOSIF010000544         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_48421_length_2012_cov_3.515585, whole genome shotgun sequence.
ACCESSION   DAOSIF010000544 DAOSIF010000000
VERSION     DAOSIF010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48421_length_2012_cov_3.515585"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLT35_13610"
     CDS             <1..632
                     /locus_tag="PLT35_13610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG30307.1"
                     /translation="AAVVNNAAIFEQTREDSVTDPLTGLPNTRFLSSHAAREIARAAR
                     LRTSVAVLVVDLDNLKPINDSFGHQAGNRALCAVAAVLRSTIRPYDVCARYGGDEFIV
                     LLSDCGVDQAEAKRTELQKAVESMSFSVGDLLKVRLSISAGAAVFPVDGESYEALLQV
                     ADSRMYGDKAARKRAKLPKTGRAPEAGTASPSPPAGPAAAAAPPTQLAH"
     gene            complement(667..1440)
                     /locus_tag="PLT35_13615"
     CDS             complement(667..1440)
                     /locus_tag="PLT35_13615"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOG30308.1"
                     /translation="MGSLTGRFGLVTGVANRRSIAWAIAKALARDGARLALTYQGERL
                     LDNVRELAAELEGPLVQPCDVTMDEQIADLYQIVGREFGGLDFLLHGTAFAPRAELSR
                     PFLETSREGFRIALDVSVYSLIALARGAAPLMAARGGGSILTLTYLGSDRVFAGYNVM
                     GVAKAALESSVRYLADNLGPQHIRVNAISAGPIKTLAASGISGFGGILQAYRERAPLR
                     RNTELEEVGEAGRFLLGPESSNITGQVLMVDSGYHVMGI"
     gene            complement(1455..1787)
                     /locus_tag="PLT35_13620"
     CDS             complement(1455..1787)
                     /locus_tag="PLT35_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30309.1"
                     /translation="MPPRPIPEFTMHKAAPAGNRRGKPFRHGQAGRHKPGAKGGPRQN
                     QGEVDGNSIHYRGGQGHFGFFGPPGAPGGRRATRPPHGQRGGQGHPPGRGPRHGFGGK
                     PPRGPKKR"
     gene            1830..>2012
                     /locus_tag="PLT35_13625"
     CDS             1830..>2012
                     /locus_tag="PLT35_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30310.1"
                     /translation="MQADLGFGRARCRRGWLTTLRRRVVLGAGEDAVSRVFGSGGLRL
                     HGRGIPTGFSPACSRLL"
BASE COUNT          256 a          739 c          714 g          303 t
ORIGIN      
        1 tcgccgccgt cgtcaacaac gccgccatct tcgagcagac ccgcgaggac tcggtcaccg
       61 atcctttgac cggcctgccg aacacgcgct tcctgtcctc gcacgcagcg cgcgaaatcg
      121 cgcgcgccgc gcggctgcgc acgtcggtcg cggtgctggt ggtcgatctg gacaacctga
      181 agccgatcaa cgacagtttc ggccaccagg ccggcaaccg cgccctgtgc gccgtggcgg
      241 ccgtgctgcg cagcacgatc cggccctacg acgtttgcgc gcgctacgga ggcgacgagt
      301 tcatcgtcct gctgtcggac tgcggcgtcg atcaggcgga ggcgaagcgg acggagctgc
      361 agaaggccgt cgagtcgatg tcgttcagcg tgggggacct gctcaaggtc cggctgtcga
      421 tcagcgcggg cgctgccgtg tttcccgtgg acggcgagtc gtacgaggcc ctgctccagg
      481 tcgccgacag ccggatgtac ggggacaagg ccgcccgcaa gcgcgcgaag ctgccaaaga
      541 ccggacgcgc ccccgaagcc gggacggcat cgccctcgcc tccggccggc ccggctgctg
      601 ccgccgcgcc gccgacgcag ctcgcccact gagcggcccg gcgcgccccg gcccgccgct
      661 tccggttcag atgcccatga cgtgatagcc cgagtccacc atcaggacct ggcccgtgat
      721 gttcgagctt tccggcccca gcaggaacct cccggcttcg cccacctcct ccagctcggt
      781 gttgcggcgg agcggcgccc gttcccggta ggcctgcagg atgccgccga acccggagat
      841 gcccgacgcg gcgagcgttt tgatgggccc ggccgaaatg gcgttcaccc ggatgtgctg
      901 cgggccgagg ttgtctgcga ggtatcgaac cgacgactcg agcgcggcct tggcgacgcc
      961 catcacgttg tagccggcga agacccggtc ggagccgagg taggtcagcg tgaggatgct
     1021 cccgccccct cgcgccgcca tcaggggcgc cgcccctcgg gccagggcaa tgagggagta
     1081 gacgctgacg tccagcgcga ttctgaagcc ctcgcggctc gtctcgagga acggccgcga
     1141 cagctccgcg cgcggcgcga aggcggtgcc gtggagcagg aaatccaggc ccccgaactc
     1201 gcggccgacg atctggtaca agtcggcgat ctgctcgtcc atcgtcacgt cgcacggctg
     1261 gacgagcggc ccctccagct cagccgccag ttcccgcacg ttgtcgagca ggcgttcgcc
     1321 ttggtacgtc agtgcgagcc tggcgccgtc gcgcgccagg gccttggcga tggcccaggc
     1381 gattgaccgc cggttggcga cgcccgtgac gagaccgaac cggcccgtga gactgcccat
     1441 tgcgccctgg gtgttcagcg cttcttggga ccgcggggag gcttgcctcc gaacccgtgc
     1501 ctcgggcccc ggcccggcgg gtgcccctgt ccgccgcgtt gaccgtgcgg cgggcgcgtc
     1561 gcccgccttc cgccgggggc gccgggcggg ccgaagaaac cgaagtgccc ctgtcctccc
     1621 cggtagtgaa tgctgttgcc gtccacctcg ccctggttct ggcgcggtcc gcccttggcg
     1681 cccggcttgt ggcggccggc ctgcccgtgg cgaaacggct tgccgcggcg gttgccagcc
     1741 ggcgcggcct tgtgcatggt gaactcgggg atcggccgcg gcggcacgtc gccttcgacc
     1801 cgggggagcg tcgctcgaat caggcggcgg tgcaggctga cctcggattc ggccgcgcgc
     1861 gctgccggcg cggctggctg accaccctgc gccggcgcgt cgtgctgggc gcgggcgagg
     1921 acgctgtcag ccgtgtcttc gggtccggcg gcctccgcct gcacggccgg ggcatcccga
     1981 ccggcttctc ccccgcctgc tcgcggctcc tg
//
LOCUS       DAOSIF010000545         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_48653_length_2005_cov_3.355385, whole genome shotgun sequence.
ACCESSION   DAOSIF010000545 DAOSIF010000000
VERSION     DAOSIF010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48653_length_2005_cov_3.355385"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLT35_13630"
     CDS             complement(<1..638)
                     /locus_tag="PLT35_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG30311.1"
                     /translation="MPSLDQTAPAPFSAGPGTVRSLETRLRETVKGAVRFDAASRALY
                     STDSSNYRQVPIGIVEPMDADDVVAAVAACREAGAPVLTRGAGTSLAGQCCNVAVVLD
                     MSVHCHAILEIDAARRQARVQPGVVLDRLREEAERVGLTFGPDPATHRWCTLGGMIGN
                     NSCGVHSVMSGKTDANIDEMEVLTYDGVRMRVGPTPPEELERRIREGGRAGE"
     gene            754..1386
                     /locus_tag="PLT35_13635"
     CDS             754..1386
                     /locus_tag="PLT35_13635"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOG30312.1"
                     /translation="MPVEHIESFFRFFYNVPELIRIAGLYGLIAIIFSETGLMVGFFL
                     PGDSLLVTAGLFAARGDLDIETLLPALILAAIAGNTTGYWIGNRAGRALYSRPNSFFF
                     RREHLVRAHEFYERHGGKAVTLAQFMPFFRTFTPVVAGAAEMAYRRFITFNVIGAVAW
                     ITSMLLTGYYLGRVVPDLQHRIHLLIAGVIFVSLLPALIAWLKEKLRAAQ"
     gene            complement(1380..>2005)
                     /gene="msrP"
                     /locus_tag="PLT35_13640"
     CDS             complement(1380..>2005)
                     /gene="msrP"
                     /locus_tag="PLT35_13640"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HOG30313.1"
                     /translation="LVSAPRRYDLDDLLAPHPLEERIYRMRCVEGWSMVIPWTGFPLK
                     DLIQRAGPLGSARFVEFTALDDPRRMPGQRVNVLDWPYVEGLRLDEALHPLTILATGM
                     YGRALPNQNGAPLRLVVPWKYGFKSIKAVVRIRLVARQPATSWGKASPREYGFYANVN
                     PGVPHPRWTQAHERRLGESGRRPTLLFNGYGDQVASLYSGLDLKRHY"
BASE COUNT          286 a          721 c          683 g          315 t
ORIGIN      
        1 atctcgccgg cgcggccgcc ctcgcgaatc cgccgctcca gctcctccgg cggcgtcggc
       61 ccgacgcgca tccgcacgcc gtcgtacgtc agcacctcca tctcgtcgat gttcgcgtcc
      121 gtcttccccg acatcaccga atggacgccg caggagttgt tgccgatcat cccgcccaag
      181 gtgcaccagc ggtgcgtggc gggatcgggc ccgaacgtca gcccgacgcg ctcggcctcc
      241 tccctcaggc ggtccagcac cacgccgggc tgcacgcggg cctgccggcg ggcggcgtcg
      301 atctcgagga tggcgtggca atgcaccgac atgtcgagca cgacggcgac gttgcagcac
      361 tgccccgcga ggctcgtgcc cgcgccccgg gtcaagacgg gcgcgccagc ctcgcggcac
      421 gccgccacgg cggcgacgac atcgtcggcg tccatcggct cgacgatgcc gatggggacc
      481 tggcggtagt tcgacgagtc ggtcgagtac aaggcccggc tggcggcgtc gaagcgcacc
      541 gcgcccttca cggtctcgcg gaggcgcgtt tcgagcgatc ggacggtgcc gggaccggcg
      601 gagaacggcg cgggagcggt ctggtcgagg ctgggcatgg cgatggccgt ccgggggcac
      661 tgcgccggca cccgcgccgg cgcgccatca gcatagcaga gcgttgtata ctcgggggat
      721 tcgcgcctct cggaagacgg cggagaaagg ccagtgcccg tggagcacat cgagagcttt
      781 ttcaggttct tctacaacgt cccggagctc atccggatcg cgggtctgta cggcctcatc
      841 gccatcatct tctcggagac cgggctgatg gtgggcttct tcctgccggg cgactccctg
      901 ctcgtcacgg ccggcctgtt cgccgcgcgg ggcgatctcg acatcgagac gctcctgccg
      961 gcgcttatcc tggcggccat cgccggcaac acgacggggt actggatcgg caaccgcgcc
     1021 gggcgcgcgc tctacagccg gccgaactcg ttcttcttca ggcgggagca cctcgtgcgg
     1081 gcgcacgagt tctacgagcg ccacggcggc aaggcggtca cgctcgcgca gttcatgccg
     1141 ttcttccgca ccttcacgcc cgtcgtcgcg ggcgccgcgg agatggccta ccggaggttc
     1201 atcacgttca acgtcatcgg cgccgtcgcc tggatcacca gcatgctgct caccggctac
     1261 tacctgggcc gcgtcgtgcc ggacctccag caccggatcc acctgctcat cgcgggcgtg
     1321 atcttcgtgt cgctgctgcc cgccctcatc gcctggctca aggagaagct gagggcggct
     1381 cagtagtggc gtttcaggtc gaggcccgag tacagcgacg ccacctggtc gccgtacccg
     1441 ttgaagagca gcgtcggccg ccgtccggac tcgcccagcc ggcgctcgtg cgcctgggtc
     1501 caccgcggat gcggcacgcc gggattcacg ttcgcgtaga aaccgtactc gcgcgggctg
     1561 gccttccccc acgaggtcgc gggctgcctc gcgacgagcc ggatccgaac gacggccttg
     1621 atgctcttga agccgtactt ccagggcacc acgagccgca gcggcgcgcc gttctggttg
     1681 ggcagggccc gcccgtacat ccccgtcgcg aggatcgtca gcgggtggag cgcctcgtcg
     1741 aggcggagcc cctcgacgta cggccagtcc agcacgttca cccgctgccc cggcatccgg
     1801 cgcgggtcgt cgagcgccgt gaactcgacg aacctcgccg acccgagggg cccagcgcgc
     1861 tggatgagat ccttgagcgg aaatcctgtc caggggatca ccatcgacca gccttcgacg
     1921 cagcgcatcc ggtagatgcg ttcctccagc ggatgcggcg ccagcaggtc gtcgaggtcg
     1981 taccggcggg gcgcgctgac gaggc
//
LOCUS       DAOSIF010000546         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_48712_length_2003_cov_3.862936, whole genome shotgun sequence.
ACCESSION   DAOSIF010000546 DAOSIF010000000
VERSION     DAOSIF010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48712_length_2003_cov_3.862936"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLT35_13645"
     CDS             complement(<1..818)
                     /locus_tag="PLT35_13645"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOG30314.1"
                     /translation="MAYNPFRHFWPKAVSVGIATLLWIMVGGEKQVERSLRAPLELQN
                     VPPGLELVGDAPSAVDVRLRGSSSGLGRLVPGDVTAVVDVSGARVGRNLVNLTPGAVR
                     VPFGIEVTYTGPATIFLVFEPQGTKRVPVVPALEGQPAPGYAVTGVRVDPSEVEIVGP
                     TSALDEVRQATTETIEIDGLKGQVRHSVAIGVANGSARLREPRNAAVTVEIQPVLVER
                     VLAIPVRLEDAGAGLAARAAPALVNVTVRGREGLVAALDAGAVEAVASLAGLAAG"
     gene            complement(818..1630)
                     /gene="cdaA"
                     /locus_tag="PLT35_13650"
     CDS             complement(818..1630)
                     /gene="cdaA"
                     /locus_tag="PLT35_13650"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOG30315.1"
                     /translation="MDTLTSMLQRSPVGLTDVLDILIAAAAIYELLKLMRGTRAMQMG
                     VGIALIVGIFYLSQWWRLDTVNWLIRNVAGYVVFAAIVLFQSEIRRALAHLGRARFFR
                     FLSRTESNDEIVEELVAAATMLASTKTGAIIAVERDAGLRNYIEGGIPLDATLTSDLL
                     VCVFQPRSPLHDGAVIIQSDRVAAAACFLPLSVNPAISRDFGTRHRAAIGLTEDVDAV
                     AIVVSEETGRIALAIDGQVERGLDADRLRTRLKALVPLRKPASERGATGTFD"
     gene            complement(1706..>2003)
                     /locus_tag="PLT35_13655"
     CDS             complement(1706..>2003)
                     /locus_tag="PLT35_13655"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG30316.1"
                     /translation="FAKRAEHSWSILAGLSGLAALGRPVLVGPSRKSFLAGAAGGAPP
                     GARDWATAAAVTAAVLAGAHVVRVHNVAALVQVARAADAVRGAVAGADPPALRS"
BASE COUNT          274 a          762 c          713 g          254 t
ORIGIN      
        1 cccgccgcaa ggcccgccag gctggcgacg gcctcgacgg cgccggcgtc gagcgccgcc
       61 acgaggcctt ctcggccccg gaccgtcacg ttcaccaggg ctggcgcggc gcgcgccgcc
      121 aggcccgcgc cggcgtcctc cagccgcacc ggaatggcca ggacgcgctc gacgaggacc
      181 ggctggatct cgaccgtgac ggccgcgttc cggggctcgc gcaggcgggc cgagccgttg
      241 gccaccccga ttgcgaccga gtggcgcacc tgccccttca ggccgtcgat ctcgatcgtt
      301 tccgtcgtgg cctgccggac ttcgtcgagc gcgctcgtcg ggccgacgat ctcgacctcg
      361 gacgggtcga cgcgcacgcc ggtcaccgca taccccggcg ccggctgccc ttccagcgcg
      421 ggtacgaccg ggacgcgctt ggtgccctgc ggctcgaaga cgaggaagat cgtcgccggg
      481 cccgtgtagg tcacctcgat gccgaacggc acccgcacgg ccccgggcgt caggttgacg
      541 aggttgcgcc cgactctcgc gcccgacacg tccaccacgg ccgtcacgtc gcccggcacg
      601 agccgcccca gccccgacga ggatccgcgg agccggacgt ccacggccga cggcgcgtcg
      661 ccgacgagct ccaggcctgg tggcacgttc tgcagctcga gcggcgcccg caggctgcgc
      721 tccacctgct tctcgccgcc gaccatgatc cacagcagcg tggcgatgcc caccgagacg
      781 gccttgggcc agaagtggcg gaaggggttg taggccatca gtcgaacgtc cctgtcgcgc
      841 cgcgttcgga cgcgggtttc cgcaggggca ccagcgcctt cagcctcgtc cggagccggt
      901 cggcgtcgag gccccgctcg acctggccgt cgatcgccag ggcgatgcgg ccggtctcct
      961 cggagacgac gatcgccacg gcgtccacgt cctcggtgag gccgatcgcc gcgcggtggc
     1021 gcgtcccgaa gtcgcggctg atcgcggggt tgaccgacag cggcaggaag caggcggccg
     1081 ctgccacccg gtcgctctgg atgatgaccg cgccgtcgtg cagcggcgac cgcggctgga
     1141 acacgcacac gagcaggtcc gacgtgagcg tggcgtcgag cgggatcccg ccctcgatgt
     1201 agttgcgcag ccccgcgtca cgctcgacgg cgatgatcgc gccggtcttc gtgctcgcca
     1261 gcatcgtcgc cgccgcgacg agctcttcca cgatctcgtc gttggactcg gtccgcgaca
     1321 ggaagcggaa gaagcgggcc cgcccgaggt gcgccagcgc gcgccggatc tccgactgga
     1381 acaggacgat ggcggcgaag acgacgtagc cggcgacgtt gcggatcagc cagttgaccg
     1441 tgtcgaggcg ccaccactgt gagaggtaga agatgccgac gatcagcgcg atgccgacgc
     1501 ccatctgcat cgcgcgcgtg cctcgcatga gcttcagcag ctcgtagatc gcggcggcgg
     1561 cgatgaggat gtccagcacg tcggtcagtc cgaccggcga ccgctggagc atcgacgtca
     1621 gtgtgtccac gctgtgatcc cgacctctgg aagtccggtg atgccggccc gtccgcccgc
     1681 tcgccggcgc gcccgcgggc gcgggtcagc tccgcaaggc gggcgggtcc gcccctgcga
     1741 ccgcgccgcg aaccgcgtcg gccgccctcg cgacctggac cagcgcggcg acgttgtgca
     1801 cgcgcacgac gtgggccccc gccagcacgg ccgccgtcac cgcggccgcc gtggcccagt
     1861 cccgcgcgcc cggcggggcg cctcccgcgg cgcccgccag aaacgacttg cgcgagggcc
     1921 cgacgagcac cggccggccc agcgccgcca gccccgagag gcccgcgagg atcgaccagc
     1981 tgtgctcggc gcgcttggcg aag
//
LOCUS       DAOSIF010000547         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_48980_length_1995_cov_3.100000, whole genome shotgun sequence.
ACCESSION   DAOSIF010000547 DAOSIF010000000
VERSION     DAOSIF010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48980_length_1995_cov_3.100000"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLT35_13660"
     CDS             complement(<1..320)
                     /locus_tag="PLT35_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30317.1"
                     /translation="MKHALVLLSLYAAAGGAVASAQSAASPAVDRAAAIRAAQRTIDD
                     EYARETMSPFTAIAVRYLEPGQTCRLGADGTGAAFDPVKPVDAAAEIGFDGGAFWVAP
                     VQDSR"
     gene            494..1216
                     /locus_tag="PLT35_13665"
     CDS             494..1216
                     /locus_tag="PLT35_13665"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOG30318.1"
                     /translation="MHVSRVLAAFVILAAGAATLAAVGPATAAAQAGEEGFTTLFNGR
                     DFTGWVYGQRPNGTENKTGVGYQIKDGILFSTEKDGGNLYTEKEYSDFAFRFEFRLTP
                     NANNGIGIRAPLVGDAAYVGMEIQVLDDTGSQYTKLEPGQYHGSIYKVVPAKRGFLKP
                     VGEWNSEEIRAEGTRITVTLNGTVIVDADLADVKDPEVLKAHADMTKPEGSRGIRNTK
                     GHIGFLGHGAHVEFRNIRIKEL"
     gene            1305..>1995
                     /locus_tag="PLT35_13670"
     CDS             1305..>1995
                     /locus_tag="PLT35_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30319.1"
                     /translation="MLRWSQAGAVAILLFLLASTGGIGAGAGAAAGDDGPPYTLTISP
                     VPAGGRVQGAGISCGAGGAACSVTMPAAMTIGLSASAEAGYTFGGWTGDCAGTSPGLW
                     LALKGARTCGAAFTPAGGSVTPPAGGTITTYAGTGTPGYGGDDGPATAARLYLPMEIA
                     ADAAGNLYIADYGNHRIRKVAAATGIISTAAGTGEAGFSGDGGPAAEAQLDNPTGVAA
                     DAAGNLFIVDRE"
BASE COUNT          328 a          707 c          700 g          260 t
ORIGIN      
        1 cgcgagtcct gaaccggcgc aacccagaac gcccctccgt cgaagccgat ctccgcggcc
       61 gcgtccaccg gcttcacggg gtcgaacgcc gcgcccgtgc cgtccgcgcc caggcggcag
      121 gtctggccgg gttcgaggta tcgcacggcg atggccgtga acggcgacat cgtctcacgc
      181 gcgtactcat cgtcgatcgt ccgctgcgcg gcacggatgg ccgccgcccg atcgacggcc
      241 ggcgaggcag ccgactgcgc gctggcgacc gcgccgccag cggccgcgta cagcgagagc
      301 agaacgaggg catgcttcac gggtcgatcc tccagatggg tcgtgcgtac attctagccg
      361 ggcacagcca ggcgacacgc acgccgcagc ggccaggatc cggggtcaga tccagtattc
      421 ctggatctga ccccacttgg tggcgaaacg ggctatcatg cggcgacccc gtttcgtatt
      481 ggaggagtca accatgcacg tgtctcgcgt actcgccgct ttcgtcatcc tggcggccgg
      541 cgccgccacg ctcgccgccg tgggccccgc cacagccgca gcacaggccg gcgaggaagg
      601 cttcaccacg ctcttcaacg gcagggactt caccggctgg gtctacggcc agcggccgaa
      661 cggcaccgag aacaagacgg gcgtcggcta ccagatcaag gacggcatcc tcttctcgac
      721 ggagaaggac ggcggcaacc tctacaccga aaaggaatac tcggacttcg cgttccgctt
      781 cgagttcagg ctgacgccca acgccaacaa cggcatcggc atccgcgcgc ccctcgtcgg
      841 cgacgcggcg tacgtcggca tggagatcca ggtgctcgac gacacaggct cgcagtacac
      901 gaagctcgag cccggccagt accacggctc catctacaag gtcgtgccgg ccaaacgcgg
      961 cttcctcaag cccgtcggcg agtggaactc ggaggagatc cgggccgagg gcacccgcat
     1021 caccgtcacg ctcaacggca cggtcatcgt ggacgcggac ctggcggacg tgaaggaccc
     1081 ggaggtgctg aaggcgcatg cggacatgac gaagcccgaa ggctcgcggg ggatcaggaa
     1141 cacgaagggc cacatcggct tcctcggcca cggcgcccac gtcgagttcc gcaacatccg
     1201 catcaaggag ctgtagacgc ggccgcagcc ggcggcctct gcatcgccac gcgctccggc
     1261 agcgtcatct gcggctgcgc attcgtcgcc gatggaggtc gagcatgctt cgctggtctc
     1321 aggcaggcgc cgtcgcaatc ctgctcttcc tcctcgcgtc gacgggaggg attggagcgg
     1381 gcgcaggcgc ggcggcgggg gacgacggcc cgccgtacac gctcaccatc tcgcccgtcc
     1441 cggcgggcgg ccgggtccag ggcgcgggga tcagctgcgg cgccgggggc gcggcgtgct
     1501 cggtgacgat gccggcggcg atgacgatcg ggctgtcggc ctcggcggag gcgggataca
     1561 ccttcggcgg gtggaccggc gactgcgccg ggacgtcgcc gggcctgtgg ctggcgctga
     1621 agggcgcgcg cacgtgcggc gcggcgttca cgcccgccgg cggcagcgtc acgccgccgg
     1681 ccggagggac catcaccacg tatgccggca ccggaacgcc cggctacggc ggcgacgacg
     1741 gcccggcaac ggccgcacgg ctctacctgc cgatggagat tgccgccgac gccgcaggca
     1801 acctgtacat cgccgactac ggcaaccacc gcatccgcaa ggtggcggcg gccaccggca
     1861 tcatctcgac cgccgcgggc acgggcgagg ccgggttctc gggcgacggc ggtcctgcgg
     1921 ccgaagcgca gctcgacaat ccgaccggcg tggccgcgga cgccgcgggg aacctgttca
     1981 tcgtggatcg ggaga
//
LOCUS       DAOSIF010000548         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_49335_length_1985_cov_4.430570, whole genome shotgun sequence.
ACCESSION   DAOSIF010000548 DAOSIF010000000
VERSION     DAOSIF010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49335_length_1985_cov_4.430570"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="PLT35_13675"
     CDS             <1..1668
                     /locus_tag="PLT35_13675"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOG30320.1"
                     /translation="PAGSTVRLTPSEILFPDGTIDRRTMGSEARGVSWWQYTKATDGE
                     ETWVPSFYYVGSRYVRAESFGPGGAPVLATTEDEQARQRRVAPDSPLAPTIVSLDAVI
                     VHSSAQPAGRFEASDPLLNRIRDLVRWAQRSNMVSVLTDCPHREKLGWLEQYHLNGPA
                     IRYEFDTSRIFTKGMNDMADSQTADGLVPDIAPEYTVFEGAFRNAAEWGAAFILVPWQ
                     QYLFNGDVALMRTHYDAMKRYAAFLESRTRNGILSDGLGDWYDLDLDKPGGAANLTPA
                     PITATAYYWLDVVTLARIAGVLGKPDDERMFREKAAAIRARYLDEFHRPAEPGYATGS
                     QAALAIPLALGLADSSTEAGVLAALVRDVEARGYATAGDVGFRSLLQALAQRGRSDVV
                     YKLVTQDTNPGYAFQLRKGATALTEAWDADRESSHNHFMMGHVTEWLYQDLAGITADE
                     RAPGFKHIVVRPQPVPGLDWVEASYESIRGPIAVRWEQAPGAFTLRVAIPANTTATVH
                     LPARGPDGVTESGTLAAASRGVSFVSFAGGRAVYRIESGRYAFESRW"
     gene            1750..>1985
                     /locus_tag="PLT35_13680"
     CDS             1750..>1985
                     /locus_tag="PLT35_13680"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30321.1"
                     /translation="MDTAGGNSGSPALNAKGEPGGAALRRHLRAGVAELPARRRDDAV
                     DSRRSPHMPRNMADVDRASRLPGEMGASTARLETA"
BASE COUNT          288 a          716 c          710 g          271 t
ORIGIN      
        1 cccgcgggaa gcacggtgcg cctgacgccg tccgagatcc ttttcccaga cggcacgatc
       61 gaccgccgca cgatggggtc cgaggcccgc ggggtgtcat ggtggcagta cacgaaggcc
      121 accgacggcg aggagacctg ggtcccgagc ttctattacg tcggcagccg ctacgtgcgc
      181 gccgagtcct tcggccccgg cggcgcgccg gttctggcga cgaccgagga cgagcaggcg
      241 cggcagcggc gcgtggcccc ggattcgccg ctggcgccga cgatcgtgtc gctcgacgcg
      301 gtcattgtcc actcgtcggc tcagccagcc ggccgcttcg aggcgtcgga cccgctgctg
      361 aaccggattc gcgacctggt gcgctgggcg cagcggtcga acatggtgtc ggtgctcacc
      421 gactgcccgc accgcgagaa gctcgggtgg ctcgaacagt accacctgaa cgggccggcg
      481 atccgctacg agttcgacac gtcgcgcatc ttcaccaagg gcatgaacga catggccgat
      541 tcccagaccg ccgacggcct ggtgccggac atcgcccccg agtacacggt gttcgagggc
      601 gcgttccgga acgcggccga gtggggcgcg gcgttcatcc tggtgccctg gcagcagtat
      661 ctgttcaacg gcgacgtcgc gctgatgcgg acgcactacg acgccatgaa gcgctacgcg
      721 gccttcctcg agtcgcgcac gaggaacggc atcctctcgg acgggctcgg cgactggtac
      781 gacctcgatc tggacaagcc cggcggggcc gccaacctga ccccggcgcc gatcaccgcc
      841 acggcgtact actggctcga cgtcgtcacc ctggcccgca tcgcgggcgt gctcgggaag
      901 ccggatgacg agcggatgtt ccgggagaag gccgccgcaa tccgcgcacg ctacctcgac
      961 gagttccacc ggccggcgga gccgggctac gccaccgggt cgcaggcggc gctcgccatc
     1021 cccctcgcgc tgggcctggc cgactcgtcc accgaggccg gcgtgctcgc cgccctcgtc
     1081 cgcgacgtcg aggcccgcgg gtatgcgacg gccggcgacg tcggcttccg ctcgctgctg
     1141 caggcgctcg cgcagcgcgg ccgctcggac gtcgtctaca agctcgtcac ccaggacacg
     1201 aaccccggct acgcgttcca gctgaggaag ggcgcgacgg cgctcaccga ggcgtgggac
     1261 gcggaccgcg agtcgtcgca caaccacttc atgatgggcc acgtcaccga gtggctctac
     1321 caggacctgg ccggcatcac ggccgatgaa cgcgccccgg gattcaagca catcgtcgtc
     1381 cgaccgcagc cggtgccggg cctcgactgg gtcgaggcgt cgtacgagtc gatccgcggg
     1441 ccgatcgccg tccgctggga gcaggcgccg ggcgcgttca cgctgcgcgt ggccataccg
     1501 gccaacacga cggcgacggt tcatctgccc gcgcgaggcc ccgacggcgt gacggagagc
     1561 ggaacgctcg ccgctgccag ccggggcgtg tcgttcgtgt cgttcgccgg cggccgggcg
     1621 gtgtaccgaa tcgagtccgg ccgctacgcg ttcgagtcgc gctggtaggc cgcggcgctg
     1681 cggaccgccg cggcggcgcc gctcctcgac acccgagtcg gtgacgcgcc ggcggactgt
     1741 ctgtccgccg tggacacggc cggcggcaac tccggatcgc cggcgctgaa cgcgaaaggc
     1801 gaaccgggcg gggctgctct tcgacggcac ctgcgagccg gtgtcgctga actgcctgct
     1861 cgacggcgcg acgacgcggt cgattcacgc cggtcaccgc acatgccgcg gaacatggcg
     1921 gacgtggaca gggccagccg gctgccgggc gagatgggcg cctcgacggc ccggctcgag
     1981 acggc
//
LOCUS       DAOSIF010000549         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_49509_length_1980_cov_3.938182, whole genome shotgun sequence.
ACCESSION   DAOSIF010000549 DAOSIF010000000
VERSION     DAOSIF010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49509_length_1980_cov_3.938182"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..813
                     /gene="rplM"
                     /locus_tag="PLT35_13685"
     CDS             382..813
                     /gene="rplM"
                     /locus_tag="PLT35_13685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG30322.1"
                     /translation="MHTYTANLKTIERRWHVIDAEGVALGRLATRAARLLMGKHRAAY
                     TPFLDTGDHVVVVNADRVKLTGRKDEQKIYRRHSGYEGGLREERAKVVRTRTPIRLVE
                     EAVRGMLPKTTLGDAMYRKLKVYAGPDHPHRGQQPTKLEVA"
     gene            815..1207
                     /gene="rpsI"
                     /locus_tag="PLT35_13690"
     CDS             815..1207
                     /gene="rpsI"
                     /locus_tag="PLT35_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG30323.1"
                     /translation="MSAIQYYGTGRRKTSTARVFLRPGNGAITVNHRQFEDFFPTAVL
                     RAEIKQPLQLTETADKFDVLATVAGGGIAGQAGAVRLGISRALCEYNSELRPALKKEG
                     LLTRDARAKERKKYGLAGARKRFQFSKR"
     gene            1237..>1980
                     /gene="rpsB"
                     /locus_tag="PLT35_13695"
     CDS             1237..>1980
                     /gene="rpsB"
                     /locus_tag="PLT35_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG30324.1"
                     /translation="MSGIAIKELLEAGVHFGHQTKRWNPKMKPYIFGQRNGIYIIDLG
                     KTAQLFGDAEAFVSRLASEGGTVLFVGTKRQAQDAVAEEAQRCGMHFVNQRWLGGLLT
                     NFTTIQRSLTRLRELEAMQTDGRYETLSKKEVAAVEKEKKKIQKNLDGIRQMAKLPDA
                     VFVIDTKKEQIAVDEARKLKIPVIGIVDTNSDPEQVDYVIPGNDDALRSIRLFTSRIA
                     DAIVAGRSLRESARAEAAADAGAHGARPAP"
BASE COUNT          366 a          660 c          699 g          255 t
ORIGIN      
        1 aaatccgccg gttccctgcc aagcggccct cgcccggccc aaagccccgg cgcctggcgt
       61 cttgcccgcc gggagccccc ggcgccgata ggcgatttcc gaggggcgcg tgcagaattg
      121 tcatcgcccc tcggccccgc gggtggcagg ttctaccgcg ctgccgcccc gacggcggcc
      181 accagcccgc aggccgggcc gccgctccgg ccggccgtct tatcggcctg acccccggag
      241 ccgtgtagag acgcgagggc gcgaggttgc accgggcgcg gccggggccg cggagaagcg
      301 cgcggttgac aaggaccagc acgggctggt acgcttttgg attgccgtgg ctgaaattga
      361 ttcagggata gagacttgca gatgcacacg tatacggcga acctgaagac gatcgagcgc
      421 cggtggcacg tgatcgatgc cgagggtgtg gcgctcggac gccttgcgac ccgggcggcc
      481 cggctgctga tgggcaagca ccgcgcagcc tacacgccgt tcctcgacac gggcgatcac
      541 gtcgtcgtcg tgaacgcgga ccgcgtgaag ctcaccgggc gcaaggacga gcagaagatc
      601 taccgcaggc acagcggcta cgagggcggc ctgcgcgagg agcgcgcgaa ggtcgtccgg
      661 acccgcacgc caatccgcct cgtggaagaa gcggtgcgcg gcatgctgcc caagacgacg
      721 ctcggcgatg cgatgtaccg gaagctcaag gtctacgcgg ggccggacca cccgcaccgg
      781 ggccagcaac ccaccaagct cgaggtagcg taacgtgtct gcaatccagt attacggaac
      841 cgggcgccgg aagacgtcga cggcccgcgt cttcctgcgg ccgggcaacg gcgcaatcac
      901 ggtcaaccac cgccagttcg aggacttctt ccccaccgcc gtgctgcgcg ccgagatcaa
      961 gcagccgctc cagctgaccg agacggccga caagttcgac gtcctggcga cggtggcggg
     1021 gggcgggatc gccggtcagg ccggcgcggt ccgcctcggc atctcccgcg cgctgtgcga
     1081 atacaacagc gagctgcggc cggcgctgaa gaaggaaggc cttctcaccc gggacgcccg
     1141 ggcgaaggag cgaaagaagt acggactggc gggtgcgcgc aagcgcttcc agttcagcaa
     1201 gcggtaaccc ggccggcatc tgtcgaggga ggaccattga gcgggatcgc catcaaggaa
     1261 ctgttggaag ccggggtgca cttcggccat cagacgaagc ggtggaaccc gaagatgaag
     1321 ccgtacatct tcggccagcg gaacggcatc tacatcatcg acctgggcaa gacggcgcag
     1381 ctcttcgggg acgccgaggc gttcgtcagc cggctcgcct ccgagggcgg cacggtgctg
     1441 ttcgtgggca cgaagcggca ggcgcaggac gcggtggcgg aggaagcgca gcgctgcggc
     1501 atgcacttcg tcaaccagcg ctggctgggc gggctgctga ccaacttcac gacgatccag
     1561 cggagcctca cccgcctgcg cgagctcgag gccatgcaga ccgacggccg ctacgagacg
     1621 ctctcgaaga aagaggtcgc cgccgtcgag aaggaaaaga agaagatcca gaagaacctc
     1681 gacggcatcc gccagatggc gaagctcccg gacgcggtgt tcgtgatcga cacgaagaag
     1741 gagcagattg cggtcgacga ggcgcggaag ctgaagatcc cggtgatcgg catcgtcgac
     1801 acgaactccg atcccgaaca ggtcgactac gtcatcccgg gcaacgatga tgcgctgcgg
     1861 tcgatccgcc tcttcacgtc gcgcatcgcg gacgcgatcg tggcgggccg ctcgctgcgc
     1921 gagtcggcgc gagccgaggc ggccgccgac gccggcgccc acggcgcgcg cccggccccg
//
LOCUS       DAOSIF010000550         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_49510_length_1980_cov_3.739740, whole genome shotgun sequence.
ACCESSION   DAOSIF010000550 DAOSIF010000000
VERSION     DAOSIF010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49510_length_1980_cov_3.739740"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1841)
                     /locus_tag="PLT35_13700"
     CDS             complement(153..1841)
                     /locus_tag="PLT35_13700"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG30325.1"
                     /translation="MITRRQFLGAAYALGGLAWTGAALVACGGRTPAAPAFSAEALMG
                     HVKVLASDQFEGRAPGTRGEDLTVAYIIEQFAQAGLQPGNPDGTYAQNVPLVGITADP
                     GAALVFRKGAAALKLAFKDDVVAWTKRVRPRVQVDASDVVFVGYGIEAPEFQWDDYKG
                     AALSGKTLVMLINDPPIRSTDDPAGLDATLFGGRAMTYYGRWTYKYEMGAAKNAAAVL
                     IVHETGPAGYPFSVVQGKVTEQFDLAAPDDNMRRPAIEGWITLERARELFALAGQDFD
                     ALKARAATRAFAPVDLGVRASMTIDSRIRRIVSRNVVGLLPGSDLALRHEYVVITAHW
                     DHYGIGPEIDGDRIYNGALDNATGVAGMIEMARRFAARRPAPKRSLLFLSVTAEEQGL
                     LGSEYYAENPLYPLNKTLAVINMDSLNIHGRTKDFTVVGLGASDLDDYARDAAAAQGR
                     TVRPDARPEQGGYFRSDHFPFAKRGVPAINAGGGEDFVGRPDGWGQRISAEYIARHYH
                     KPSDEVRPEWDLAGALEDLQVYYAIAERAARTARWPEWKAGSEFKARRDAMLGR"
BASE COUNT          266 a          738 c          647 g          329 t
ORIGIN      
        1 cgcgtaaccg tcactcgagc cgccgctgaa tttcaacagc cgacgggaca tcgccgccgg
       61 cggcgaagta ggccctctgt cccgagctgg cggcccggcg gcggttcggc cgggccggct
      121 tccgcggcgg cagccggccc gcgagccgcc gctcagcgcc ccagcatcgc gtcgcgcctg
      181 gccttgaact cgctgcccgc cttccactcg ggccaccggg ccgttcgcgc ggcgcgttcc
      241 gcgatggcgt aatagacctg gagatcttcc agcgcgccgg cgaggtccca ttccggccgg
      301 acctcgtccg acggcttgtg gtagtgcctc gcgatgtact ccgccgagat ccgctgcccc
      361 cacccgtctg ggcggccgac gaagtcctcc ccgccgcccg cgttgattgc cggcaccccc
      421 cgcttggcga acgggaagtg atccgaccgg aagtagcccc cctgctccgg ccgcgcgtcc
      481 ggacggaccg tgcggccctg cgccgcggca gcgtcgcgcg cgtagtcgtc caagtcggag
      541 gcccccaggc cgaccacggt aaagtccttc gtccggccgt ggatgttgag gctgtccatg
      601 ttgatgacgg ccagcgtctt gttcagcggg tagagcgggt tctcggcgta gtactcggac
      661 ccgaggaggc cctgctcctc ggcggtgacc gacaggaaca gcagagaccg cttcggcgcg
      721 ggccggcggg cagcgaaccg ccgcgccatc tcaatcatcc cggccacgcc ggtggcgttg
      781 tcgagggcgc cgttgtagat ccggtccccg tcgatctccg ggccgatccc gtagtggtcc
      841 cagtgcgccg tgatgacgac gtactcgtgg cgaagggcca ggtcgctccc cggcagcagc
      901 ccgacaacgt ttctcgagac gatgcgcctg atgcggctgt cgatggtcat cgacgcccgc
      961 acgcccaggt cgaccggcgc gaacgcccgg gtggccgcac gcgccttcag ggcgtcgaaa
     1021 tcctggccgg ccagcgcgaa caactcccgc gcccgctcca gcgtgatcca gccctcgatg
     1081 gcggggcgtc gcatgttgtc gtccggcgcc gccaggtcga actgctccgt caccttgccc
     1141 tgcacgaccg agaagggata cccggccggc ccggtctcgt gcacgatcaa cacggcggcg
     1201 gcgttcttcg cggcgcccat ttcgtacttg taggtccagc gtccgtaata ggtcatcgcc
     1261 cgtccgccga agagcgtcgc gtcgagtccc gccgggtcgt ccgtgctccg gatcggcggg
     1321 tcgttgatca gcatcacgag cgtcttgccc gacagcgcgg cgcccttgta gtcgtcccat
     1381 tggaactcgg gcgcctcgat gccgtagccg acgaacacga cgtccgaggc gtcgacctgg
     1441 acgcgcggcc gcacccgctt cgtccacgcc acgacatcgt ctttgaacgc cagcttcagc
     1501 gcggcggcgc ccttgcggaa cacgagcgcg gcgcccgggt cggcggtgat gccgaccagc
     1561 ggcacgttct gcgcgtaggt gccgtcgggg ttgccgggct gaaggcccgc ctgcgcgaac
     1621 tgctcgatga tgtaggcgac ggtgaggtct tcgccgcgcg tgccgggcgc ccggccctcg
     1681 aactggtccg atgccagcac cttgacgtgc cccatgagcg cctcggcgga gaacgcgggc
     1741 gcggccggcg tccgcccgcc gcacgcgacg agcgccgcac ctgtccatgc caggccgccg
     1801 agcgcgtacg cggcacctag aaactgacgt cgtgtgatca tccgaagccc ttgccttccc
     1861 tgctccgcgc cgcgtactcc gtatcccgcg ctccacgttc tggattctga attctggatt
     1921 ccggattctg acgtctgtac ctcctttacc tcctttacct cctacttccc ctcgtcccct
//
LOCUS       DAOSIF010000551         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_49683_length_1975_cov_2.217188, whole genome shotgun sequence.
ACCESSION   DAOSIF010000551 DAOSIF010000000
VERSION     DAOSIF010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49683_length_1975_cov_2.217188"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..510
                     /locus_tag="PLT35_13705"
     CDS             109..510
                     /locus_tag="PLT35_13705"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOG30326.1"
                     /translation="MIERVFVYGTLMSGFDRQRRAGIDARMRFVSRGWMTAALYDLGL
                     FPAAIPAGEGRVCGELYEIDDDPSVVRTLDQIEGFRPDSPETSLYVRAEAGITTEDGT
                     QVPAWVYFYNAPLGRAERIDSGDYLEHLRPK"
     gene            complement(538..900)
                     /locus_tag="PLT35_13710"
     CDS             complement(538..900)
                     /locus_tag="PLT35_13710"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG30327.1"
                     /translation="MRADMDGEKREAPRLDTLGQLTGEVSVLAPIVVYDLSALGARLE
                     CAFPLVLGSAHEIRLHLGPDAVVVRARVVHCQIAEIGHELVRYVAGVEFLERPPHVDA
                     ALRAYAARLGRGRTTGAP"
     gene            938..1489
                     /gene="folE"
                     /locus_tag="PLT35_13715"
     CDS             938..1489
                     /gene="folE"
                     /locus_tag="PLT35_13715"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOG30328.1"
                     /translation="MEDLIRQLLVDVGEDPSREGLVDTPRRVSRSMRFLTGGYAVDVH
                     ALINSALFDVDYSEMVIVKDTDFFSLCEHHLLPFFGKCHVAYIPRTKVIGLSKIPRLV
                     DAFARRLQVQERLTNQIAETIQEAVDPLGVAVVMEATHLCMAMRGVEKQRSFAVTSAM
                     LGAFRDRDRTRMEFLELIGRRHA"
     gene            1619..>1975
                     /locus_tag="PLT35_13720"
     CDS             1619..>1975
                     /locus_tag="PLT35_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30329.1"
                     /translation="MVRACAIVVAAMLAAVPASGQTRSPSAKPSAKPAASSARRVEAA
                     AVTCPSPLGIGVATKRAFCDVPIGRTPADGITITIPPHRGPTTLTFDLHNRQIFSEDL
                     VRAKRSYARLTATIGVL"
BASE COUNT          289 a          695 c          687 g          304 t
ORIGIN      
        1 ccggcgttcc atagactgct tggctaatcc cagttcccgc ccgcggcacg ccggccgcgc
       61 cccttggcgc cgccggcagg gcgttgatcc ccacggggag gcctgtcggt gatcgagcga
      121 gtcttcgtgt acggaacgct catgagcggg ttcgaccggc agcgccgcgc aggaatcgac
      181 gcgcggatgc ggttcgtgtc gcgcggctgg atgacggcgg cgctctacga tctgggcctg
      241 ttcccggccg cgattcccgc tggcgaaggc cgggtctgcg gagagctgta cgagatcgac
      301 gacgacccct cggtcgtccg gacgctcgat cagatcgaag ggttccgccc ggacagcccc
      361 gagaccagcc tctacgtgcg agccgaagcg gggatcacga ccgaggacgg gacccaggtg
      421 ccggcgtggg tctacttcta caatgcgccg ctcggccgcg ccgaacggat tgactccggc
      481 gactacctcg agcatcttcg cccgaagtaa ggggtgcgcg gccggcggcc cggcccgtca
      541 gggggcgccg gtcgtccggc cccggccgag gcgggcggcg tacgcccgga gcgcagcatc
      601 gacgtgcggc ggccgctcga ggaactcgac cccggccacg taccgcacca gttcatggcc
      661 gatctcggca atctggcaat gcacgacgcg cgcccggacg acgacggcgt cgggtccgag
      721 gtgcagccgg atttcgtggg cgcttccgag gaccagcggg aaggcgcatt cgagccttgc
      781 gccgagcgcg ctgaggtcgt acaccacgat gggcgccagc acgctgacct cgcccgtcag
      841 ctgaccgagc gtgtccaggc gcggggcttc ccgcttctcg ccgtccatat cggcgcgcac
      901 tataccatgc cgccggcttg atatgatgga cctcgtcatg gaagacctga tccggcagct
      961 gctggtggac gtgggcgaag acccgtcgcg cgagggcctg gtggacacgc cgcgccgcgt
     1021 ctcgcgttcg atgaggttcc tgaccggcgg atacgcggtc gacgtccacg cgctgatcaa
     1081 cagcgcgctg ttcgacgtgg actacagcga gatggtgatc gtcaaggaca cggatttctt
     1141 cagcctctgc gagcaccacc tcctgccgtt cttcggcaag tgccacgtgg cgtacatccc
     1201 gcgcacgaaa gtgatcgggc tcagcaagat cccgaggctc gtggacgcct tcgcgcggcg
     1261 gctgcaggtg caggagcgtc tcacgaacca gatcgccgag accatccagg aggcggtcga
     1321 tccgctcggc gtggccgtcg tcatggaggc cacgcacctc tgcatggcca tgcggggcgt
     1381 ggagaagcag cggtcgttcg ccgtgacgag cgcgatgctc ggcgccttcc gcgatcgcga
     1441 ccgcacgcgc atggagttcc tcgaactcat cggccgccgc cacgcctagc ggcggccccc
     1501 gtccgatgcc cgggctgctc ccggcggtca tcctcctcgg gacgcatctc gtgccggcgg
     1561 tcggcgtgtg gcggctctcc tgatcgcctc cagagcgccc gctggtatac tgcgatcgat
     1621 ggtgcgtgcg tgcgcgatcg tggtagcggc catgctggcg gccgtcccgg ccagcggcca
     1681 gacccgctcg ccgtcggcca agccgtcggc caagccagcg gcctcgtcgg cccgccgcgt
     1741 cgaggcggcc gcggtcacgt gtccatcacc gctcggaatc ggcgtcgcga cgaagcgcgc
     1801 cttctgcgac gtgccgatcg ggcggacgcc ggccgacggc atcaccatca ccattccgcc
     1861 gcaccggggc ccgacgacgc tgacgttcga cctgcacaac cggcagatct tctcggagga
     1921 tctcgtgcgg gcgaagcggt cgtacgcgcg cctgacggcc acgatcggcg tgctc
//
LOCUS       DAOSIF010000552         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_50352_length_1956_cov_2.870594, whole genome shotgun sequence.
ACCESSION   DAOSIF010000552 DAOSIF010000000
VERSION     DAOSIF010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50352_length_1956_cov_2.870594"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLT35_13725"
     CDS             complement(<1..317)
                     /locus_tag="PLT35_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30330.1"
                     /translation="MSKSTAVLLAVCAGVAVPAMLAAQPPAAPPDGANPLLQPWSTPF
                     GVPPFADIKPAHFVPAIKAGIAAQRREIEAIAGNPAPPTFANTIEALEDAGELLGRVG
                     PVFS"
     gene            500..1210
                     /locus_tag="PLT35_13730"
     CDS             500..1210
                     /locus_tag="PLT35_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOG30331.1"
                     /translation="MAELYREGPHVPADAVYPVVRTSARPLTLLPGHDPDWASAAAIA
                     WGPQEYRTSFRALWNDAGRALRFDAGDTRPWHTMTQRDDPIWEEEAVEIFLDPARLGR
                     HYAEVEISPLNVVTDLHILEPWPRLRGDKSWDWSGLESTVVPGACGGLAPGSWVALAW
                     LPWPGLAGMCPEVAAAVPPAPGDRWRFNVFRIKRPNGPAEPERGAVYAAWSTPPGPSF
                     HDPSAFRTMAFAGPEHGR"
     gene            1467..1787
                     /locus_tag="PLT35_13735"
     CDS             1467..1787
                     /locus_tag="PLT35_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOG30332.1"
                     /translation="MGRKLYVGNLPYSTGEQDLQDLFSACGQVDSVSVMRDMATGRAR
                     GFAFVEMATDADAQKAIAELHDKPFGGRNLTVNEARPRTERPGGGFGGSDRGGRGGRR
                     ESRW"
BASE COUNT          300 a          692 c          717 g          247 t
ORIGIN      
        1 gagaacaccg ggcccacccg cccgagcagc tcgccggcgt cctcgagcgc ctcgatggtg
       61 tttgcgaagg tgggaggcgc cggattgccc gcgatggcct cgatctcccg gcgctgggcg
      121 gcgatgcccg ccttgatcgc gggcacgaag tgcgccggct tgatgtcggc aaacggcggc
      181 acgccgaacg gcgtggacca cggctgcagg agcgggttcg cgccgtcggg cggcgcagcg
      241 ggcggctggg cggcgagcat cgcgggaacc gcgacgccgg cgcagacggc gagtaagaca
      301 gcggtgctct tgctcatgga acggcctctc aaagaccccc gccgatgacg aggtgccgtg
      361 aatctaccgc ccggcccgga ggccggtcaa ggctcccccc agggggcggc gcgaggacgg
      421 cgatcgaggc cgcgcttgac cggccgggag cgaggccgga tactggggaa gctccccggc
      481 cctgacagga cccggcccga tggctgagtt gtatcgcgaa ggaccgcacg ttcccgcgga
      541 cgccgtgtac ccggtcgtcc ggacctcggc acgtccgctc accctgctgc ccggacacga
      601 ccccgactgg gcgtcggccg cggcgatcgc ctggggaccg caagagtacc gcacctcctt
      661 ccgcgcgctg tggaacgacg cgggccgcgc gctccggttc gacgccggcg acacccgccc
      721 gtggcacacg atgacccaac gcgacgatcc aatctgggag gaggaggcgg tcgagatctt
      781 cctcgacccg gcgcgtctcg gacgccatta cgccgaggtc gagatcagcc cgctcaacgt
      841 cgtgaccgac ctgcacatcc tcgagccgtg gcccaggctg cgcggcgaca agagctggga
      901 ctggagcggc ctcgagagca ccgtggtgcc gggggcctgc ggagggctgg cgcccggcag
      961 ctgggtggcc cttgcctggc tgccctggcc ggggctcgcg ggcatgtgtc ccgaggtggc
     1021 cgccgccgtg cccccggccc cgggcgaccg ctggcgcttc aacgtgttcc ggatcaagcg
     1081 gcccaacggg ccggccgagc ctgaacgcgg cgcggtgtac gcggcctggt ccaccccgcc
     1141 gggcccgagc ttccacgatc cgtcggcgtt caggacgatg gccttcgcgg gcccggaaca
     1201 cgggcggtga cggccgtggc ggcccgcctc ggcgcgccgc cacggcccaa tcgtgctatc
     1261 attccccggt ctggtggaag cccgccttct gtagcacccg cgaagagagc cgaatcgagt
     1321 cgccatcgac tcgctcctgc cgcgacgtac tcgctcagtc acgagctgcc atcgaagggc
     1381 ggtccacgcg gaatcgccga gtgtgaatga cggccggcac cgccggccgt ccagcgtctc
     1441 ctggagcgag acgaggagca agagcaatgg gcagaaagct gtatgtcggc aacctcccct
     1501 actcgacggg cgagcaggac ttgcaggacc tgttctccgc ctgcgggcag gtggactcgg
     1561 tgagcgtgat gcgcgacatg gccacgggcc gcgcgcgcgg gttcgcgttc gtcgaaatgg
     1621 cgactgatgc cgacgcgcag aaggccatcg ccgaactgca cgacaagccg ttcggcgggc
     1681 gcaacctgac ggtcaacgaa gcgcgcccgc ggaccgagcg tcccggcggc ggattcggcg
     1741 ggagtgaccg cgggggccgc ggcggacggc gcgagtcgcg ctggtagtcg cctcgcacga
     1801 catcgcgagt cgaacggggg gccggcgcga cgcgcccgcc ccccgttcgc cgtcagtccg
     1861 ggccgcgcca agcgcggtcc ccttaatctg gagccagcgt gaaccgaacc agaccttccc
     1921 tgcagaagcg ggaacgcgag aaggccaagc gggaga
//
LOCUS       DAOSIF010000553         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_50378_length_1955_cov_4.157895, whole genome shotgun sequence.
ACCESSION   DAOSIF010000553 DAOSIF010000000
VERSION     DAOSIF010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50378_length_1955_cov_4.157895"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLT35_13740"
     CDS             complement(<1..937)
                     /locus_tag="PLT35_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG30333.1"
                     /translation="MASLLVRGGTVLTMSDDLRVVRGEVSVRDGRIEAVGAGLPGSWD
                     TIIDATDALVLPGFVQSHVHLCQTLFRGLADDLPLLEWLRTRIWPLEAAHTPGTLRAS
                     ARLAALELLTGGTTTVLTMETVHDTEAVFEAVAEIGLRAVIGKCMMDADAAAPRRLLE
                     PTQASIDESLSLRKRWHGAAGGRIRAAFAPRFAVSCSQDLLEAVGALAAGHGALVHTH
                     ASEQQPEVELIRQRTGRRNVQYFEDIGLASERLCLAHCIWIDEAEQEILASRGVKVLH
                     CPSSNLKLGSGIAPVPELRARGVSVSLGADGAACSN"
     gene            complement(952..1767)
                     /locus_tag="PLT35_13745"
     CDS             complement(952..1767)
                     /locus_tag="PLT35_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOG30334.1"
                     /translation="MHEQVLSAASGAIQRGEAAALVTVVSAHGSTPQRPGAKMLVHAD
                     GRTVGTIGGGATEHDAVGKAQTAIRTGRAELLHYDLDTGRAGGEGGLCGGRMDVFVDP
                     LDTPPSLFVAGAGHVGQQLAALAATVGFAVHVVDDREAFANAGRFPSAASIAVADIAS
                     HLRSAPITSSAYVVVVTRGHADDLAAMRALAGREAKYIGMIGSKAKVAQIVDALALEG
                     VAPDWLRRVRAPIGLRIGAVSPAEIAVSIVAELVAVRRGEATDASAAMSMSGR"
BASE COUNT          276 a          714 c          697 g          268 t
ORIGIN      
        1 ggttgctgca cgccgcgccg tcggcgccca aggacaccga cacgccgcgg gccctcagtt
       61 ccggaacggg agcgatgccg ctgccgagct tcaggttgga gctgggacag tgcagcacct
      121 tcactccgcg cgacgccagg atctcctgct cggcctcgtc aatccagatg cagtgcgcta
      181 ggcacagccg ctccgaggcg aggccgatgt cctcgaagta ctgcacgttg cggcggcccg
      241 tccgctggcg gatgagttcg acctccggct gctgctccga tgcgtgcgtg tgcaccagcg
      301 cgccgtggcc cgccgccagc gcgcccacgg cctcgagcag gtcctgcgag cacgagacgg
      361 cgaagcgcgg cgcgaaggcg gcgcgaatcc tgccgcccgc cgcgccgtgc caccgtttcc
      421 gcagcgacag gctctcgtcg atcgaggcct gcgtgggctc cagaaggcgc cgcggcgccg
      481 ccgcgtcggc gtccatcatg cacttgccga tgacggcccg cagcccgatt tcggcgacgg
      541 cctcgaagac cgcctcggtg tcgtgcacgg tctccatcgt cagcactgtc gtcgtgccgc
      601 ccgtgagcag ctcgagcgcg gcgagccgag ccgacgcccg cagggtcccg ggcgtgtggg
      661 ccgcctcgag gggccagatg cgcgtgcgca gccactcgag gagggggagg tcgtcggcca
      721 gcccgcggaa cagcgtctgg cacaggtgaa cgtgcgactg cacgaacccg ggcagcacca
      781 gcgcgtccgt cgcgtcgatg atggtgtccc acgagcctgg cagccccgcc ccgacggcct
      841 cgatgcgccc gtccctgact gacacctctc cccgcacgac gcggaggtcg tcgctcatcg
      901 tcaggaccgt accgccccgg acgagcaggg aagccatgtc caccgctccg gttatcggcc
      961 gctcatggac atcgccgcag atgcgtccgt cgcctcgccg cgccggaccg ccacgagttc
     1021 cgcgacgatg ctcacggcga tttcggcggg cgacaccgcg ccgatcctca gtccgatcgg
     1081 cgcgcgcacg cggcgcagcc agtcgggggc gacgccctcg agcgccagcg cgtcgacgat
     1141 ctgagcgacc ttggccttgc tgccgatcat gccgatgtac ttggcctcgc gaccggcgag
     1201 ggcccgcatc gccgccaggt cgtccgcgtg gccgcgcgtc accaccacga cataggccga
     1261 gctggtgatg ggcgccgacc gcaggtgcga ggcgatgtcg gccacggcga tcgacgccgc
     1321 ggacgggaag cggccggcgt ttgcgaaggc ctcgcgatcg tcgacgacgt ggacggcgaa
     1381 gccgaccgtg gcagcgagcg cggcgagttg ctggcccacg tgcccggcgc cggcgacgaa
     1441 caggctcggc ggcgtgtcga gcggatcgac gaagacgtcc atccggcctc cgcagagccc
     1501 tccttccccg ccagcccggc cggtgtccag gtcgtagtgg aggagttccg cccggcccgt
     1561 ccgaatagcc gtctgggcct tgccgaccgc atcgtgctcg gtcgccccgc cgccgatcgt
     1621 tccgacagtc cgcccgtcgg cgtgaacgag catcttcgcc cctgggcgct gtggcgtcga
     1681 gccgtgcgcc gacaccaccg tgaccagcgc ggctgcttcg ccgcgctgga tggctccgga
     1741 cgcggcggat agaacctgtt cgtgcatagg tgcattatag ggccggggat tccggattcg
     1801 gggccccctc cggcctgcgt gagccgcagc gcgctcgaga cgggcggggc ggagccgggc
     1861 gagaagaaca gcccctagaa ccggtgcagg tcgcagggaa gggtcggctc ggtgccggcg
     1921 aggaatgctt cgcgcaggac cctcgggcac gcggg
//
LOCUS       DAOSIF010000554         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_50492_length_1952_cov_3.086979, whole genome shotgun sequence.
ACCESSION   DAOSIF010000554 DAOSIF010000000
VERSION     DAOSIF010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50492_length_1952_cov_3.086979"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..>1952
                     /gene="mrdA"
                     /locus_tag="PLT35_13750"
     CDS             146..>1952
                     /gene="mrdA"
                     /locus_tag="PLT35_13750"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG30335.1"
                     /translation="MTGPPALQRRRLASRLNALSVCVVATFAALAVAFWYFQVVMNAE
                     YVRLAENNHQRRLALRAPRGILYDREFRPLVENRDAYTISILREHSRDLDRVVRRLAE
                     ATGVAEQRIRDELDRHRSEPRYRPVVVIGDASLAQVSAVLARRLDFELPDVVVERVPT
                     RRYPALGVGAHLLGYVGEASETQVQAGGLRPGAVVGQSGVERAYNTELMGTDGERRVM
                     VNSVGREITTLQEIAASEGRRLQLTIDLDLQMAAEHAFHALGYQGAAAFLDPRSGEVL
                     ALVSLPAYDPNHFATGIDRATWIRLNTDVLKPLQNRAIQGRYSPGSTFKAVVAAAALE
                     EGIITPDFTVTCRGGATFYGRFFQCHSKSGHGPVDLRHAIEKSCNTYFYTVGNMVGIE
                     RLHKWAALLGLAGKTGIDLPNEVESLVPSPEWKKARTGEKWYAGETISVAIGQGQVSV
                     TPISLANLYAAVANGGPHCPPRLLKAINDGQGWRPPDEVAAPEGPRLKPETVAALHDG
                     FWLVVNGAGTGGRARIPGHDVAGKTGTAQVISLEGGRRAAGRTERDLRDHGWFAFFAP
                     RDNPEIAGVVFAEHGEHGSSAAPIARHVLETYFAKR"
BASE COUNT          278 a          680 c          736 g          258 t
ORIGIN      
        1 accatccgct tctcgaggcc gggtcccagg cgatcgcgaa cgcggcggcg ggcgtgctgc
       61 tgttggccgc cggggacttc gcgccgtcgg tgaagcgctg ggccgaggcc gcggcggagc
      121 gccggcggaa gcggaggtcg ttccggtgac gggccctccc gcgctccagc ggcggagact
      181 ggcgtcgcgg ctcaacgcgc tttccgtgtg cgtcgtggcg acgttcgccg cgctcgcggt
      241 cgcgttctgg tacttccagg tggtcatgaa cgcggagtac gtgaggctgg ccgagaacaa
      301 ccaccagcgc cggctcgccc tgcgcgcgcc ccgcggcatc ctctacgacc gcgagttccg
      361 cccgctcgtg gagaaccggg acgcgtacac gatctcgatt ttgcgcgagc acagccgcga
      421 cctcgaccgc gtcgtccgcc gcctcgcgga agccaccggc gtggccgagc agcgcatccg
      481 cgacgagctg gaccgtcacc gcagcgagcc gcgctaccgg cccgtcgtcg tgatcggcga
      541 cgcgagcctc gcgcaggtgt cggcggtgct ggcgcgccgg ctggacttcg agctgccgga
      601 cgtcgtcgtc gagcgcgtgc cgacgcgccg ctacccggcg ctcggcgtcg gggcgcacct
      661 gctgggctac gtgggcgagg ccagcgagac gcaggtccag gccggcggcc tgcgcccggg
      721 cgccgtcgtc ggccagtcgg gcgtggagcg ggcctacaac accgagctca tgggcaccga
      781 cggcgagcgg cgcgtgatgg tgaacagcgt cggccgcgag atcacgaccc tgcaggagat
      841 tgcggcgtcg gaggggcggc ggctccagtt gacaatcgac ctcgacctcc agatggcggc
      901 cgagcacgcg ttccacgccc tggggtacca gggggccgcg gcgttcctcg atcctaggtc
      961 gggggaggtg ctcgcgctgg tcagcctgcc ggcgtacgac ccgaaccact tcgccaccgg
     1021 catcgatcgg gccacctgga tccgcctcaa caccgacgtg ctgaagccgc tgcagaaccg
     1081 cgcgatccag ggccggtact cgccggggtc gaccttcaag gcggtggtgg cggccgccgc
     1141 gctcgaggaa gggatcatca cgccggactt cacggtgacc tgccgcggcg gcgcgacctt
     1201 ctacgggcgg ttcttccagt gccacagcaa gagcggccac ggccccgtcg acctgcgcca
     1261 cgccatcgag aagtcctgca acacgtactt ctacaccgtc ggcaacatgg tcgggatcga
     1321 acgcctgcac aagtgggccg cgctgctcgg gctcgccggg aagacgggga tcgatctgcc
     1381 gaacgaggtc gagagcctcg tgccctcgcc ggagtggaag aaggcccgca cgggggagaa
     1441 gtggtacgcc ggcgagacga tctcggtggc gatcggccag gggcaggttt cggtcacccc
     1501 gatttcgctg gcgaacctgt acgccgcggt ggcgaacggc ggaccgcact gcccgccgcg
     1561 cctgctcaag gcgatcaacg acgggcaggg ctggcgcccg cctgacgagg tggcggcgcc
     1621 ggaagggccg cggctgaagc cggagaccgt cgcggcgctc cacgacgggt tctggctcgt
     1681 cgtcaacggc gccgggaccg gcggccgggc gcgcattccc gggcacgacg tggcggggaa
     1741 gacgggcacg gcgcaggtga tctcgctcga gggcggccgg cgggcggccg ggcggacgga
     1801 gcgggacctg cgcgatcacg gctggttcgc cttcttcgcg ccgcgcgaca accccgagat
     1861 cgccggcgtc gtgttcgcgg agcacgggga gcacggatcg agcgcggcgc cgatcgcccg
     1921 ccacgtcctg gagacgtact tcgccaaacg gg
//
LOCUS       DAOSIF010000555         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_50519_length_1951_cov_3.280591, whole genome shotgun sequence.
ACCESSION   DAOSIF010000555 DAOSIF010000000
VERSION     DAOSIF010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50519_length_1951_cov_3.280591"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..803
                     /locus_tag="PLT35_13755"
     CDS             465..803
                     /locus_tag="PLT35_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30336.1"
                     /translation="MPLLIAIALVVVLALTLGIGVIGIVRLKQGAEPGSPVDEFCGGT
                     EALDEIHTLFDGIYARWNGLGPWEAPLDMPDEERAVWIRERRYARLGWIVTDIVIRGG
                     AWLNRRFGRG"
     gene            810..1277
                     /locus_tag="PLT35_13760"
     CDS             810..1277
                     /locus_tag="PLT35_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30337.1"
                     /translation="MAVPWRAGVVVGVDPGVTTGLCAYVDGEFAGGEEATTYGQVEEF
                     ISLWQPYTIVIEDFVLRRGRACEYHAPIRMIGVVEYICGLRGIPMVVQSPSILGMTRH
                     LVQGVHRSEHVRSACAHVVYHLSRRRRRDRGADKQPRADDPRVEESPGDAPGV"
     gene            1240..>1951
                     /locus_tag="PLT35_13765"
     CDS             1240..>1951
                     /locus_tag="PLT35_13765"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOG30338.1"
                     /translation="MSKRVPATLRAYEGQRRHHYSFPVCGDILRRLLANDPDLGISER
                     LWERLDAISRQQAEALEIAGMDDACGDQRLRPYQRVAVKWLSTVRRGILADEQGLGKT
                     VEACCAAAVLAPERALVICPTAKIGDWAEHVERWVPGARVARLEGDAQARADEVSRWR
                     QIGGYLVSNYARAQIEARGLQGADLVVIDEAHKVRNRAAGQTRAIRGIGRSAGALFLV
                     TATPNINGAEDIWTLLDMV"
BASE COUNT          304 a          600 c          707 g          340 t
ORIGIN      
        1 tcgaaggtct caccgagatg caatactccc gcccgtcctg gctgatgtcg ggcgtgttcg
       61 ctatcgtcgc ggcccttgta tcggggctgc tcgtctacgc gctgaagtaa ttcacctaat
      121 tttttatcac tcttcgtagt agtagatctc tttatcccgc aggtcgaccc atccgatcgc
      181 cctgccgccg cgaattatga cggggttccc cgtattcgcg cgggtccggc cggacagttg
      241 gtcagttggg tcgagtttgc cgagcaaccg ccgcatctcc tcgacggtga tcaatgtgtg
      301 ttcagtaggt tccatcgctc ttagggttta tacctcagaa gtataaattg catggtgatg
      361 gccaacgccg gcgcatcgcc aggtatcgcg catcggcctc gccccgtgcc tctgccgcat
      421 catttttcac ctggtactcc tcatagccct gtgagagaga catcatgccg ctgctgattg
      481 ctatcgcact ggtcgtggtc ctcgcgctta ccctgggcat cggggtgatc gggatcgtga
      541 ggctgaagca gggcgccgag cccgggagcc ccgtggacga gttctgcgga ggcaccgagg
      601 ccctggacga gatacacacg ctgttcgacg gtatctacgc tcggtggaac gggctcgggc
      661 cgtgggaggc gccgctggac atgccagacg aggagcgggc cgtgtggatc cgcgagcgcc
      721 ggtacgcccg gctcgggtgg atcgtgacag acatcgtcat ccgcggcggg gcctggctga
      781 accgccggtt cgggcggggc tgagcattgg tggccgtgcc gtggcgggcg ggcgtcgtgg
      841 tgggcgtgga cccgggggtg accaccgggc tctgcgccta cgtcgacggg gagttcgccg
      901 ggggcgagga ggccaccacc tacggccagg tcgaggaatt catatccctg tggcagccat
      961 acactatagt catcgaggac tttgtgctgc ggaggggcag ggcgtgcgag taccacgccc
     1021 cgatccgcat gatcggggtc gtggagtaca tctgtgggct gcggggcata cccatggtgg
     1081 tgcagtctcc gtcgatcctc ggcatgaccc ggcacctggt ccagggggtt cacaggagcg
     1141 agcacgtcag gtcggcgtgc gcgcacgttg tgtatcacct cagcaggagg cgacggcgtg
     1201 acagaggtgc agataaacag ccgagggcgg atgaccctcg tgtcgaagag agtcccggcg
     1261 acgctccggg cgtatgaggg ccagaggcgc caccactatt ctttccccgt gtgcggggac
     1321 atcctgaggc ggctcctggc caacgacccg gacctgggga tctccgagcg gctctgggag
     1381 cggctcgacg cgatctcccg gcagcaggcc gaggcgctcg agatcgccgg catggacgac
     1441 gcctgcggcg accagaggct ccggccgtac cagcgcgtgg ccgtgaagtg gctgtccacc
     1501 gtccgccgcg gcatcctggc cgacgagcag ggcctcggca agaccgtcga ggcgtgctgc
     1561 gccgcggcgg tgctggcgcc cgagagggcg ctggtcatct gcccgacggc caagatcggc
     1621 gactgggcgg agcacgtcga gaggtgggtg cccggcgccc gggtggcccg cctcgagggc
     1681 gacgcacagg ccagggccga cgaggtctcg cgctggcggc agatcggggg ctacctggtg
     1741 tcgaactatg cccgggcgca gatcgaggcc cgggggctgc agggcgcgga cctcgtggtc
     1801 atcgacgagg cgcacaaggt ccggaaccgg gcggcggggc agaccagggc gatccggggg
     1861 atcggccggt ccgccggggc gctgttcctg gtgacggcca cgccgaacat caacggggcc
     1921 gaggacatct ggacgctcct cgacatggtc g
//
LOCUS       DAOSIF010000556         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_50553_length_1950_cov_3.360950, whole genome shotgun sequence.
ACCESSION   DAOSIF010000556 DAOSIF010000000
VERSION     DAOSIF010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50553_length_1950_cov_3.360950"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLT35_13770"
     CDS             complement(<1..347)
                     /locus_tag="PLT35_13770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOG30339.1"
                     /translation="MDSGAGSRGPGPEHANPVREATTGFSVTPDLRDGVLVLCLGNDL
                     LRDDGVGWAVADALEARLAEPGVASPALPVCVRRSALSGFYLLDELTGWDRAVVVDAV
                     QTGRHPAGTVLSFP"
     gene            complement(347..1825)
                     /locus_tag="PLT35_13775"
     CDS             complement(347..1825)
                     /locus_tag="PLT35_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOG30340.1"
                     /translation="MSQRITIDPITRLEGHGKIEIFLDDRGDVANAYLQIPELRGFEH
                     FCVGRAAEEMPRITQRICGVCPSAHHMASARALDDLYHVEPPPAAHKLRELFYNLFIF
                     EDHTLHFYFLGGPDFIVGPAAPAGERNILGVIGKVGLEIGGKVIEARKIARGFMAEMA
                     GKVIHPVFGLPGGVSKPLKAETVDRLKAASQRLVDFAQFSLQAFHDIVLGNQAYVDTI
                     LADYYAHKTNYLGTVDANGRVNFYRGEFRVVDPSGARLAQFPPKQYLDYIGEHVEPWS
                     YMKFPFLKTPGWKGFVDGPDSGLYRVAPLARLNVADGMATPIAQAHYEEFFRVLGGKP
                     VHSTLATHWARLIEALQATETVQALANDPEILSPVVRTLPESAPTEGIAGVEAPRGTL
                     IHHYKTDPNGILTMVNLIVATVHNAGPIQMSVDKAAKGVIRGGKVDDALLNMVEMAFR
                     AYDPCFGCAAHSLPGEMPLRATIRDAQGAVVRVLERTSRLPG"
BASE COUNT          287 a          656 c          707 g          300 t
ORIGIN      
        1 gggaaggaga gcacggtgcc cgccgggtgc cgcccggttt gcaccgcgtc caccacgacg
       61 gcgcggtccc atccggtcag ctcgtcgagc aggtagaagc ccgacaacgc gctgcggcgc
      121 acgcagaccg gcaatgcggg gctcgcgacc ccgggctcgg ccagccgggc ttcgagggca
      181 tcggcgacgg cccagccgac gccgtcgtca cgcagcagat cgttccccag gcacaacacc
      241 agcaccccgt cccgcaggtc gggcgtcacg gagaacccgg tcgtggcctc gcgaaccggg
      301 ttcgcgtgtt caggccccgg cccccggctc ccagcccccg agtccatcac ccgggcaacc
      361 tcgacgtccg ctccagcacc ctcacgaccg ctccctgggc gtcgcggatc gtggcccgca
      421 gcggcatttc gcccggcagc gagtgggcgg cgcacccgaa gcagggatcg taggcccgga
      481 acgccatctc gaccatgttc aggagcgcgt cgtccacctt gccgccgcgg atgacgccct
      541 tggcggcctt gtccacggac atctggatgg gccccgcgtt gtgcaccgtc gcgacgatga
      601 ggttgaccat cgtcaggatg ccgttcgggt cggtcttgta gtggtggatg agcgtgccgc
      661 gcggcgcctc gacgccggcg atgccctcgg tcggcgccga ttcggggagc gtccgcacga
      721 ccgggctgag gatctccggg tcgttcgcca gggcctggac cgtctccgtc gcctgcagcg
      781 cctcgatgag gcgcgcccag tgggtggcca gcgtgctgtg cacgggcttg ccgccgagca
      841 cccggaagaa ctcctcgtag tgggcctgcg cgatgggcgt ggccatgccg tcggccacgt
      901 tgaggcgagc caggggcgcg acccggtaga ggccgctgtc ggggccgtcc acgaagccct
      961 tccaccccgg ggtcttgagg aacgggaact tcatgtacga ccacggctcg acgtgctcgc
     1021 cgatgtagtc gaggtactgc ttcggcggga actgcgcaag gcgcgcgccg gagggatcga
     1081 ccacgcggaa ctcgccccgg tagaagttga cccggccgtt ggcgtccacc gtgcccaggt
     1141 agttcgtctt gtgggcgtag tagtcggcca ggatcgtgtc cacgtaggcc tggttgccga
     1201 gcacgatgtc gtggaacgcc tgcagggaga actgcgcgaa atcgacgagg cgctgggagg
     1261 cggccttgag gcggtccacc gtctcggcct tgagcggctt gctgacgccg ccgggcaggc
     1321 cgaacaccgg gtggatcacc ttgccggcca tttcggccat gaacccgcgg gcgatcttgc
     1381 gcgcctcgat gaccttgccg ccgatctcga ggcccacctt gccgatgacg ccgaggatgt
     1441 tccgctctcc cgccggcgca gcgggcccga cgatgaagtc ggggccgccg agaaagtaga
     1501 agtgcagggt gtggtcctcg aagatgaaca ggttgtagaa gagctcgcga agcttgtggg
     1561 cggccggcgg cggctccacg tggtacaggt cgtcgagggc gcgggccgac gccatgtggt
     1621 gcgccgacgg gcagacgccg cagatgcgct gcgtgatgcg gggcatctcc tccgcggctc
     1681 ggccgacgca gaagtgctcg aagccgcgca gctccgggat ctgcaggtac gcgttcgcga
     1741 cgtcgccccg gtcgtcgagg aagatctcga tcttgccgtg gccctcgagg cgggtgatgg
     1801 ggtcgatcgt gatgcgctgg ctcatacggt cgctccaggg cgccgccggt gcagcaggga
     1861 cttcggcagc gagaaccggt agaacgtgcc cacgggatcg gggatctggt cgaggagcct
     1921 gtcgatctcc gcctcggtct tcggcgcgag
//
LOCUS       DAOSIF010000557         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_50666_length_1947_cov_3.529070, whole genome shotgun sequence.
ACCESSION   DAOSIF010000557 DAOSIF010000000
VERSION     DAOSIF010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50666_length_1947_cov_3.529070"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLT35_13780"
     CDS             complement(<1..697)
                     /locus_tag="PLT35_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG30341.1"
                     /translation="MKFALFSDILGMAFGTMRANKMRAALTILGVVIGITAIVGMTAL
                     IRGFDESLRDSIRQLGPNTIFVAKFSGLSVSSGVEFKDLVKRPVLTVADARAIEREAP
                     SAGMVDVWLGMWGRPVRERAYYKGEKTKELSVLGVSENYAAVNFLSLEGGRFFTESEV
                     QHRRPVVVLGDTPVKALFPHVDPVGKRVRVGAVQYEVVGYVGKRPRPFGNSGQDDFLV
                     VPYTTFQKQFGWSR"
     gene            complement(706..>1947)
                     /locus_tag="PLT35_13785"
     CDS             complement(706..>1947)
                     /locus_tag="PLT35_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG30342.1"
                     /translation="REEVDAVSRILEGILIALRAIWSSKLRSFMSVLGNIVAVTSIIA
                     VVSLIQGLNATVTDAIVSEVGADSFIVDRYGITRSEEDFEAVRSNPPVTLRDAEAIRR
                     YSPLVRAVMAESTRSGQVSYRDQVLETISIRGVSPEYVSFSSYNAERGRLLSATEVER
                     NRNVVLLGWGTADRLFGHANPLDRVIKIEGVHYRVIGVNEKKGAIFGQSQDEFAVIPL
                     GGFQKIFGARPYLQLNVKPVHPDLVSDAMQDATVALRIERRLKPKEKDNFGLFTSDSI
                     LDIYHQATSGIFAVLVGVVALSLLVGGIVIMNIMLMVVTERTFEIGLRKAIGAKRRDI
                     MLQVLAESVTLSVAGGVTGTALGFFLAWLISKASPLPAAVQPWSVALGIGITAIVGLI
                     FGVYPAMRAAALDPIEALRRE"
BASE COUNT          315 a          657 c          670 g          305 t
ORIGIN      
        1 agcggctcca cccgaactgc ttctggaagg tcgtgtaggg gacgacgagg aagtcgtcct
       61 ggccgctgtt gccgaacggc ctcgggcgct tgccgacgta gccgacgacc tcgtactgga
      121 cggcgcccac gcggacgcgc ttgccgacgg gatcgacgtg ggggaagagc gccttgacgg
      181 gcgtgtcgcc gaggacgacg acgggccggc ggtgctggac ctcggactcg gtgaagaagc
      241 ggccgccttc gagcgacagg aagttcaccg cggcgtagtt ctccgagacg ccgaggaccg
      301 acagctcctt ggtcttctct cccttgtagt aggcccgctc gcgcaccggc ctgccccaca
      361 tgccgagcca gacgtccacc attccggccg acggcgcctc gcgctcgatg gccctggcgt
      421 cggccacggt gagcacgggc cgcttcacca agtccttgaa ctcgacgccg gagctgacgc
      481 tgaggcccga gaacttcgcg acgaagatgg tgttcggccc gagctgccgg atcgagtcgc
      541 gcagcgactc gtcgaacccg cggatgagcg ccgtcatgcc gacaatcgcc gtgatgccga
      601 tgaccacccc gaggatggtc agcgcggcgc gcatcttgtt cgcccgcatg gtgccgaagg
      661 ccatgccgag gatgtccgag aagagcgcga acttcatcgc cacggctact ctctcctcag
      721 cgcctcgatg gggtcgaggg cggcggcgcg catggcgggg tagacgccga agatcaggcc
      781 gacgatggcc gtgatcccga tgccgagggc gaccgaccac ggctgcacgg cggccggcag
      841 cgggctggcc ttcgagatga gccaggccag gaagaacccg agggccgtgc cggtgacgcc
      901 gcccgccacc gacagggtga ccgactcggc cagcacctgg agcatgatgt cgcggcgctt
      961 ggcgccgatg gccttccgca gcccgatctc gaacgtccgc tcggtgacca ccatcagcat
     1021 gatgttcatg atgacgatgc cgccgacgag gagcgagagg gccaccaccc cgacgagcac
     1081 cgcgaagatg ccgctggtcg cctggtggta gatgtccagg atcgagtccg acgtgaacag
     1141 gccgaagttg tccttctcct tcggcttcag gcgccgctcg atcctgagcg ccaccgtcgc
     1201 gtcctgcatc gcgtccgaga cgaggtccgg gtgcacgggc ttgacgttca gctgcaggta
     1261 cggccgggcg ccgaagatct tctggaagcc gccgagcggg atcaccgcga actcgtcctg
     1321 cgactgcccg aagatcgccc ccttcttctc gttgacgccg atcacccggt agtggacgcc
     1381 ctcgatcttg atgacccggt cgagggggtt cgcgtggccg aacaggcggt cggccgtgcc
     1441 ccatccgagg agcacgacgt tccggttccg ctcgacctcg gtggcgctca gcaggcgccc
     1501 gcgctcggcg ttgtaggagg agaagctgac gtactcgggc gacacgccgc ggatgctgat
     1561 ggtctcgagg acctggtccc ggtagctcac ctggcccgac cgcgtgctct cggccatgac
     1621 ggcgcgcacc agcgggctgt accgccggat ggcctcggcg tcccgcaggg tcaccggggg
     1681 gttgctccgg accgcctcga agtcctcctc gcttcgcgtg atcccgtagc gatcgacgat
     1741 gaaggagtcg gcgcccacct ccgacacgat cgcgtcggtg acggtggcgt tgaggccctg
     1801 gatgagggac accaccgcga tgatggacgt caccgcgacg atgttgccga gcacgctcat
     1861 gaacgagcgc agcttgctgc tccagatggc ccggagcgcg atgaggatgc cctcgaggat
     1921 ccggctcacg gcgtctactt cttcgcg
//
LOCUS       DAOSIF010000558         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51007_length_1938_cov_4.100372, whole genome shotgun sequence.
ACCESSION   DAOSIF010000558 DAOSIF010000000
VERSION     DAOSIF010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51007_length_1938_cov_4.100372"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLT35_13790"
     CDS             <1..283
                     /locus_tag="PLT35_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30343.1"
                     /translation="GAFNADGGRVLLQRRRRRHSVARRSFTPTFKTYQVRAGDGEGRG
                     CGDTLSLALERRLNRFAFRAVSLFGVTGPARRVAGAEAASHGQWPSHRP"
     gene            complement(259..1080)
                     /locus_tag="PLT35_13795"
     CDS             complement(259..1080)
                     /locus_tag="PLT35_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOG30344.1"
                     /translation="MRIALVQQKAGADKADNVARGLSALGAAARHGAKLVCYAELSFE
                     PFHPQRPATPAALAAAEPIPGPTTELFLAKAREHGIVVVLNLFERDGDRTYDSSPVID
                     ADGRLLGVTRMIHITEYSCFHEQGYYAPGDTGAPVYDTAIGRVGVAICYDRHYPEYMR
                     AMAVRGAELVVVPQAGALGEWPEGLYEAEMRVAAFQNGYFTALCNRVGPEECLTFGGE
                     SFVCGPDGRVIARAPSLEETVLYADVDLAEVPRSHARQLFLRDRRPDLYGRWLGH"
     gene            complement(1411..>1938)
                     /locus_tag="PLT35_13800"
     CDS             complement(1411..>1938)
                     /locus_tag="PLT35_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOG30345.1"
                     /translation="DGAAPKPGGEVYVDRVDPGYVVKGVGDLNGDTKADIVWQHATLG
                     DVWTWTMNGATRLGEVYVGTVAPATGYHIQGVADFDGNGKADLVWWHETRGEVWLWEI
                     NDGALVAQAWVGTVPDTNYQIAATGDYNGDTKADLLWRNEVNGEVWVWLMAGSTKLSE
                     TWVATVPDRRYRIIR"
BASE COUNT          280 a          724 c          635 g          299 t
ORIGIN      
        1 cggggcgttc aatgccgacg gtgggcgggt tctgctgcag cgacgacggc ggcgccacag
       61 cgtcgcacgg cggtcgttca cgcccacctt caagacgtac caggtgcgcg ccggggacgg
      121 cgaggggagg ggttgcgggg acacgctcag cctcgcgctc gagaggcgcc tcaaccgctt
      181 cgccttccgc gcggtcagcc tcttcggcgt caccggcccc gcccgccgcg tggcgggggc
      241 ggaggcggcc agccatggtc agtggccgag ccatcgtccg tagagatcgg ggcgccgatc
      301 gcggaggaac agctgccgtg cgtgcgaccg ggggacctcc gcgagatcga cgtctgcgta
      361 gagaaccgtc tcctcgagcg aaggcgcacg cgcgatgacg cggccgtccg gcccgcagac
      421 gaacgactcg ccgccgaacg tcaggcattc ctccgggccc acccggttgc agagcgcagt
      481 gaagtagccg ttctggaagg ccgccacgcg catctcggcc tcgtagaggc cctcgggcca
      541 ctcgccgagc gcgccggcct ggggcacgac gaccagctct gcgccccgca ccgccatcgc
      601 ccgcatgtac tccgggtaat gccggtcgta gcagatggcc acgcccaccc tgccgatggc
      661 cgtgtcgtag accggggctc ccgtgtcgcc cggcgcgtag tagccctgct cgtgaaagca
      721 ggagtactcg gtgatgtgaa tcatgcgggt gacgccgagg agcctgccgt cggcgtcgat
      781 caccggggag ctgtcgtacg tccggtcgcc gtcccgctcg aagaggttca ggacaacgac
      841 gatgccgtgt tcccgcgctt tggcgagaaa aagctccgtt gtggggccgg gaatcggctc
      901 ggccgccgcc agggcggccg gcgtcgccgg cctctgcgga tggaagggct cgaaggacag
      961 ctccgcgtag cagacgagct tcgcgccgtg tctcgcggcc gcaccgagcg ccgacaggcc
     1021 gcgcgcgaca ttgtcggctt tgtcggcgcc ggccttctgc tgaaccaggg cgatacgcat
     1081 gtggcctccc ggcggccggc ccgccctgga ccggcgccgc acgaggattc taccgccccg
     1141 gacgcgccgg cccgggggga gcaacgcagg gatcccgtcg cagggccttc agcgcgcgac
     1201 actgaaccac gatggtgcgc gaggcgagag gacgcacgcg ggtggtcgcc gctcggcgac
     1261 cgcccgcgag ttgaggtcac acccgtcttc caacgtcacg ccggcgcgtc gcgtgtcgtg
     1321 ccgccgtcgc cgccactcag acacaccccg cgtgccggat cccgccttcc ccaggcgcag
     1381 tcgaggcgaa tcccgaatcc tgaacgggtc tcagcggatg atccgatatc tccgatccgg
     1441 caccgtcgcc acccatgtct ccgagagctt cgtcgagccg gccatcagcc acacccacac
     1501 ttcgccgttc acctcgtttc gccacaagag gtcggccttc gtgtcgccgt tgtagtcgcc
     1561 cgttgcggcg atctggtaat tcgtgtcagg caccgtcccc acccacgcct gcgccaccag
     1621 cgccccgtcg tttatctccc acagccacac ttcgcctcgg gtctcgtgcc accacaccag
     1681 gtccgccttc ccgttcccat cgaagtccgc cacgccctgg atgtggtaac cggtggctgg
     1741 tgctaccgtc cccacgtaca cctcgccgag ccgtgtcgcg ccgttcatgg tccaggtcca
     1801 cacatcgccc agcgtcgcgt gctgccatac gatgtcagcc ttcgtgtcgc cgttcaggtc
     1861 ccctacgccc ttcaccacgt agcccgggtc cacccggtcg acgtacacct ccccgcccgg
     1921 tttcggcgca gccccgtc
//
LOCUS       DAOSIF010000559         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51024_length_1938_cov_2.714817, whole genome shotgun sequence.
ACCESSION   DAOSIF010000559 DAOSIF010000000
VERSION     DAOSIF010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51024_length_1938_cov_2.714817"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLT35_13805"
     CDS             <1..845
                     /locus_tag="PLT35_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG30346.1"
                     /translation="RTAGQFRSSVELVEVYASVSDAAGTSVRGLQAADFEVLEDGRPQ
                     PIQAFAAGDFPLSLAIALDHSASMAGSRLVQATSAVRRFLGRLRPEDQVMLLGIAGRV
                     EVLAPLSTDRPQQHQALDLLRPWSTTSLYDALIAGVGLIQPAGGRRALVVLSDGEDRY
                     SEATPAEVLDRARHSDVLVYPLAIGGTAPRVFAEVAAITGGRSFAPKNPRAIDEALAS
                     LTAELRDQYLLGYAPPRADDGRPEDGWRSITVRVRGRQGLRIRARDGYFAKRAATGAG
                     GRPS"
     gene            842..1237
                     /locus_tag="PLT35_13810"
     CDS             842..1237
                     /locus_tag="PLT35_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30347.1"
                     /translation="MNKVVVRFADKHAAKGFTNDFFPGRESFHLSPASESGAEPVVIR
                     VSDLKAVFFVHDLAGNREKTDRKEFDPAHPAVGRRIKVVFKDGEVFVGTTHGYQPGRQ
                     GFFVVPADATSNIQRCYVVTAATESVSFL"
     gene            complement(1454..>1938)
                     /locus_tag="PLT35_13815"
     CDS             complement(1454..>1938)
                     /locus_tag="PLT35_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOG30348.1"
                     /translation="GRAKAGIIPGHICQPGRVGVVSRSGTLTYEAIHQLTRLGLGQTT
                     CIGIGGDPLIGTSFVDALGLFQADPETEGIVLIGEIGGTAEEEAAAFITAHVTKPVVC
                     FICGQTAPPGRRMGHAGAIIAGGKGTAAEKMAALSAAGAHVVRSPADIGQAMADALGA
                     "
BASE COUNT          264 a          702 c          681 g          291 t
ORIGIN      
        1 ccaggacggc cggccaattc cgctcctcgg tggagcttgt cgaggtgtac gccagcgtca
       61 gtgacgcggc gggcacgagc gtccgcggcc tccaggcggc ggatttcgaa gtgctcgagg
      121 acggacgccc gcagccgatt caggcgttcg cggcaggcga cttcccgctc tcgctggcga
      181 tcgccctcga ccacagcgcc agcatggccg ggtcgcgcct cgtgcaggcg acaagcgccg
      241 tgcgccggtt cctggggcgg ttgcggcccg aggaccaggt catgctcctt gggattgccg
      301 gccgcgtgga ggtgctggcc ccgctgtcga ccgaccgccc gcagcagcac caggccctcg
      361 acctcctgag gccctggagc acgaccagcc tgtacgacgc cctcatcgca ggcgtgggcc
      421 tcattcagcc ggccggcggg cgccgcgcgc tggtcgtcct gtcggacggc gaggaccgct
      481 acagcgaggc gacgccggcg gaggtgctcg atcgcgcccg gcactcggac gtgctggtgt
      541 acccactggc aatcggcggg accgctccgc gtgtgttcgc ggaagtggcc gcgatcaccg
      601 ggggccgatc gttcgcgccg aagaacccga gagccatcga cgaggcgctc gccagcctga
      661 ccgccgagct gcgcgaccag tacctgctgg gctacgcgcc gccgcgcgcg gacgacgggc
      721 gtccggaaga cggctggcgc agcatcacgg tgcgggtccg cgggcgccaa ggcctccgga
      781 tcagggcgcg agacggttac ttcgcgaagc gtgcagcaac gggagccggc gggaggccct
      841 catgaacaag gtcgtggtcc ggttcgccga caagcacgcc gcgaagggct tcacgaacga
      901 cttctttccg ggtcgggagt cgtttcacct gagcccggcc tcggaatccg gcgccgagcc
      961 cgtcgtcatc cgcgtcagcg acctcaaagc ggtcttcttc gtccacgacc tcgccggcaa
     1021 ccgcgagaag accgaccgga aggagttcga ccccgctcat ccggctgtcg gccgccggat
     1081 caaggtggtc ttcaaggacg gcgaggtctt cgtgggaacc acccacgggt accagccggg
     1141 ccgccagggg ttcttcgtcg tgcctgccga cgccacgtcg aacatccagc gctgctacgt
     1201 cgtaacggcg gcaaccgagt ccgtcagctt cctctgatcg ccctgtcgtc cggcgaagcg
     1261 gccgccacgc tggcagcgga ctcggcacga gcgccagaaa gcggcggcaa gccggccccg
     1321 ctcgtgcgtc ctgcctcccg cctccggcgt cccgcttcct gctgccagcg cctgcctgcc
     1381 gtctgccgtc gcctccgaca tgcgactgcc gtccggatgc cgccggcctt gtgaggccga
     1441 gcttgtcttc cgtctaggca ccgagtgcgt ccgccatcgc ctggccgatg tccgccgggc
     1501 tcctgacgac gtgcgcgcct gcggccgaga gcgcggccat cttctcggcg gccgtgccct
     1561 tgccgccggc gatgatggcg cccgcatggc ccatccgcct tccgggcggc gcggtctggc
     1621 cgcagatgaa gcagacgacg ggcttcgtga cgtgcgccgt gatgaaagcc gccgcctctt
     1681 cctcggccgt cccgccgatt tcgccgatga gcacgatgcc ctcggtttcg gggtcggcct
     1741 ggaagaggcc gagggcgtcc acgaagctcg tgccgatgag cggatccccc ccgatgccga
     1801 tgcacgtcgt ctggccgagg ccgagccgcg tgagctggtg gatcgcctcg taggtcagcg
     1861 tgccgctgcg cgagacgact ccgacccggc ccggctggca gatgtggccg gggatgatgc
     1921 cggccttggc gcggccgg
//
LOCUS       DAOSIF010000560         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51090_length_1936_cov_3.707071, whole genome shotgun sequence.
ACCESSION   DAOSIF010000560 DAOSIF010000000
VERSION     DAOSIF010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51090_length_1936_cov_3.707071"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /gene="coaD"
                     /locus_tag="PLT35_13820"
     CDS             complement(<1..334)
                     /gene="coaD"
                     /locus_tag="PLT35_13820"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOG30349.1"
                     /translation="MSAPRERVAVYPGSFDPLTNGHVDIILRGARLFDRIVVAILRHA
                     EKTPLFSAAERVAMATEVFQDHPNVEVESFDGLLVDYAERRRALAIVRGLRAVSDFEY
                     ELQMALMNR"
     gene            complement(331..906)
                     /gene="rsmD"
                     /locus_tag="PLT35_13825"
     CDS             complement(331..906)
                     /gene="rsmD"
                     /locus_tag="PLT35_13825"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOG30350.1"
                     /translation="MRVIAGRYKGRRLAQPAWPGLRPTSDRLKESVFAILEPVLEGAR
                     VLDGFAGSGALGIEALSRGASEVVFVDGDARAAALIARNLAHCGITQGYVMMRAEFAQ
                     ALGRLPAGTRFDLALLDPPYDHAGLDAALAAAAGRMAEGGVIVLEHATRRRPPGRAGR
                     FAAGRLVSAGDSSVTFYREAPAPAASGREGP"
     gene            complement(949..>1936)
                     /locus_tag="PLT35_13830"
     CDS             complement(949..>1936)
                     /locus_tag="PLT35_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG30351.1"
                     /translation="EGLASGRVPFVVGTHALVQQDVVFSQLGLAVVDEQHRFGVMQRA
                     GLREKGWRPDLLVMTATPIPRTLALTVYGDLDVSAIRDLPPGRQPVRTLVLPESRRDE
                     AHRAVREELAAGRQAYIVYPLVEVSAKIDVRAATEMADHLAAEVFPEYRLGLLHGRLR
                     PEEKDRVMRAFAAGDVHVLVATTVIEVGIDVPNATVMLVEHAERFGLAQLHQLRGRVG
                     RGPHPSACLLVHQEPLGEGGEARLRALAQTTDGFVIAERDLDLRGPGDVFGLRQSGMP
                     TLRSGDLLRDRDIMEQARRIARDLLERDPAASAIVAEARRSWSGRFGLASVG"
BASE COUNT          274 a          769 c          648 g          245 t
ORIGIN      
        1 ggcggttcat cagcgccatc tgcagctcgt actcgaagtc cgacaccgcg cgcagtcccc
       61 gcacaatcgc cagcgcccgc cgccgctcgg cgtagtcgac cagcagcccg tcgaaggact
      121 cgacttcgac gttcgggtga tcctggaaca cctcggtcgc catcgcgacg cgctccgccg
      181 ccgagaacag gggggtcttc tcggcgtgtc gcaggatcgc gacaacgatc cggtcgaaga
      241 ggcgcgcccc acgcaggatg atgtccacgt gcccgttcgt gagcgggtcg aacgacccgg
      301 gatagacggc cacccgttcg cgaggtgcgc tcatggaccc tccctcccgc tggcggccgg
      361 cgccggcgcc tcgcggtaga acgtcacgct gctgtcgccg gcggacacga gccgtcccgc
      421 cgcaaaccgg cccgcccgcc cgggcggccg ccgccgggtc gcgtgctcga gcacgatcac
      481 gccgccctcc gccatccgtc ccgcggcggc ggcaagcgcg gcgtccaggc ccgcatgatc
      541 atacggggga tccaacaggg cgagatcgaa ccgcgtgccg gcgggcagcc ggccgagcgc
      601 ctgcgcgaat tcggcgcgca tcataacata gccctgcgtg atgccgcagt gcgccaggtt
      661 gcgcgcgatg agcgccgccg cgcgtgcgtc cccgtccacg aagaccacct ccgacgcccc
      721 tcggctcaac gcctcgatgc ccagtgcccc ggacccggca aacccgtcca gcacccgcgc
      781 cccctcgagc acgggctcga ggatggcgaa caccgactcc ttgagccggt ccgacgtggg
      841 ccgaagcccc ggccaggccg gctgcgccag ccggcggccc ttgtaccggc ccgcgatgac
      901 gcgcatccgc ccctcccgtc ccgccccggc ggcccgcccg gttcgccctc agccgacgct
      961 cgccaggccg aaccggccgg accagctccg tcgggcctcc gccacgattg cgctcgccgc
     1021 cgggtcccgc tccagcaggt cccgcgcgat ccgcctcgcc tgctccatga tgtcccggtc
     1081 ccggaggagg tcgcccgagc gcagcgtcgg cattcccgat tgccggaggc cgaacacgtc
     1141 cccgggcccg cgcaggtcga ggtcccgctc ggcgatgacg aacccgtcgg tggtctgggc
     1201 gagggcgcgg agccgggcct cgccgccctc tccgaggggc tcctggtgca cgagaaggca
     1261 ggccgacggg tgggggccgc gccccacgcg cccgcgcaac tggtgcaact gcgccagccc
     1321 gaaccgctcc gcgtgttcga cgagcatcac cgtcgcgttc ggtacgtcga tcccgacttc
     1381 gatgaccgtc gtcgccacga gcacgtgcac gtcgccggcc gcgaaggccc tcatcacgcg
     1441 gtccttctct tccggccgca ggcgcccgtg cagcaggccc aggcggtact cgggaaagac
     1501 ctcggccgcc aggtgatcgg ccatctcggt cgcggcccgc acgtcgattt tcgccgagac
     1561 ctccaccagg ggatacacga tgtacgcctg ccgtccggcc gccagttcct cgcggacggc
     1621 ccgatgcgcc tcgtcgcgcc tcgactccgg gagcaccagt gtgcgcacgg gctgacggcc
     1681 cggcggcaga tcgcggatcg ccgacacgtc gaggtcgccg tagacggtca gcgccagcgt
     1741 gcgcgggatg ggcgtggctg tcatcacgag cagatcgggg cgccagccct tctcccggag
     1801 cccggcgcgc tgcatgacgc cgaaccggtg ctgctcgtcg accaccgcga gcccgagctg
     1861 cgagaagacg acatcctgct gcacgagcgc gtgggtcccg acgacgaagg gcacccgccc
     1921 ggacgccagg ccctcg
//
LOCUS       DAOSIF010000561         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51259_length_1931_cov_4.978678, whole genome shotgun sequence.
ACCESSION   DAOSIF010000561 DAOSIF010000000
VERSION     DAOSIF010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51259_length_1931_cov_4.978678"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLT35_13835"
     CDS             complement(<1..1064)
                     /locus_tag="PLT35_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG30352.1"
                     /translation="MTQSIDFDVMTPGDGERGGVSKSDRQLLTTLFALGREVTSVLDL
                     AELYRKLPGLIARVTDFHAFAVYVLDERQGRLRIAHAEGYPPGSRSLRLRPGQGLVGR
                     AAADGRPLLANDVSKEPDYLRIVPGTRAELAVPLRRKGRSIGVLNLLSRNLHEFTPAE
                     VETLRIFGAAVATAIENARLFESERRYAETISTLAEIGREFAGILELDHLLERIAHRV
                     RRLIEYRTFGILLVDEDTGEIRMRHAVRYNEGTAAKALKIGEGLVGYAVLHKTPVVVD
                     DVAADPRYIKVMDDVQSELVVPLLVKDRCVGVFDLESPERASFTQEHVEVLTSLAASA
                     AVAIDNARLYEELRRSDVSTS"
     gene            1185..1652
                     /gene="fabZ"
                     /locus_tag="PLT35_13840"
     CDS             1185..1652
                     /gene="fabZ"
                     /locus_tag="PLT35_13840"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOG30353.1"
                     /translation="MDQLPFDQTVIQRILPHRYPFLLVDRITEFAGGTHIVGTKTVSL
                     NERCLSREPGRTPTLPPTILTEAVAQVGAIMVLSKPENRDKLIFFMGIDRVKFHRPVY
                     AGDQVVVFARVKRLRSKMGVLEGVARVNGGIVAAGTMTFALGPAEGGQATLRD"
     gene            1662..1910
                     /locus_tag="PLT35_13845"
     CDS             1662..1910
                     /locus_tag="PLT35_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30354.1"
                     /translation="MEACPACGSRRVYRSRTRSVFERIRRQVTTKRPFRCHNCNWRGW
                     VAEAARPVTPRQPAKASPPPPDLSAVDSALDGPTKRDG"
BASE COUNT          291 a          679 c          687 g          274 t
ORIGIN      
        1 gaggtgctga cgtcgctgcg ccgcagctcc tcgtagagcc gagcgttgtc gatggccacg
       61 gccgcgctgg cggcgagcga cgtcagcacc tcgacgtgct cctgcgtgaa cgacgcgcgc
      121 tccgggctct ccaggtcgaa cacgcccacg caccggtcct tcacgagcag cggtactacg
      181 agttccgact gcacgtcgtc catgaccttg atgtagcggg gatccgccgc gacgtcgtcc
      241 acgacgacgg gcgtcttgtg cagcaccgcg tagccgacga ggccctcgcc gatcttcagc
      301 gccttggccg ccgtgccctc gttgtaccgg accgcgtggc gcatgcggat ttcgcccgtg
      361 tcctcgtcga cgaggaggat gccgaacgtg cggtactcga tcaggcgccg cacgcgatgg
      421 gcgatccgct cgagcaggtg gtcgagctcg aggatgcccg cgaactcgcg accgatctcc
      481 gccagcgtcg agatcgtctc ggcgtaccgg cgctcggact cgaacaggcg cgcgttctcg
      541 attgccgtcg caaccgccgc gccgaagatc cggagcgtct ccacctcggc cggcgtgaac
      601 tcgtggaggt tccggctcag gaggttcagc accccgatgg accggccctt gcggcgcagc
      661 ggcaccgcga gctcggcgcg cgtgcccggc acgatcctca agtagtccgg ctccttcgag
      721 acgtcgttcg ccagcagggg gcgcccatcc gccgccgcgc ggccgaccag cccctggccg
      781 ggtctcaggc gcagcgaccg gctgccggga gggtagcctt ccgcgtgggc gatgcggagg
      841 cggccctgcc gctcgtcgag gacgtacacg gcgaaggcgt ggaagtcggt gacgcgagcg
      901 atgagccccg gcagcttgcg gtagagctcc gcgagatcga gcaccgatgt cacttcgcgc
      961 ccgagcgcga agagcgtcgt caggagctgg cggtcgctct tcgacacgcc tccccgctcg
     1021 ccgtcgcccg gcgtcatgac atcgaaatcg atggactggg tcacgaggcg attatactta
     1081 cagggaccaa gggcccgggg ctcggggctg gcccgtcacg cgcgccccgg aggcagcgag
     1141 ggccgcgacc cgcgggcgcc gagcgcctgc gtcagagccg agccatggac cagctcccgt
     1201 tcgatcagac cgtcatccag cgcatcctgc cgcaccgcta cccgttcctg ctcgtcgacc
     1261 gcatcaccga gttcgccggc ggcacgcaca tcgtcgggac gaagaccgta tcgctcaacg
     1321 agcggtgcct gtcgcgcgag cccggcagga cgccgacgct gccgccgacg atcctcacgg
     1381 aggccgtcgc gcaggtcggg gccatcatgg tgctgtcgaa gcccgagaac cgcgacaaac
     1441 tcatcttctt catgggcatc gaccgcgtga agttccacag gcccgtgtac gcgggggatc
     1501 aggtggtggt cttcgcgcgg gtcaagcgcc tgcgatcgaa gatgggggtg ctcgagggcg
     1561 tcgcgcgcgt gaacggcggg attgtggccg ccggcacgat gacctttgcg ctcggcccgg
     1621 cggaaggcgg gcaggcgacg ctgagggact gaggaggacg catggaggcc tgcccagcct
     1681 gcggctcccg ccgggtgtac cgatcgcgga cgcggagcgt cttcgagcgg atccgccgcc
     1741 aggtcacgac gaagcggccc ttccggtgcc acaactgcaa ctggcgcgga tgggtcgccg
     1801 aggcagcccg gcccgtgacg ccccggcaac cggcgaaggc gagcccgccg ccgccggatc
     1861 tgagcgcggt cgactccgcg ctggacgggc ctacgaagcg cgacgggtag ccggcggccc
     1921 cgccgaggcg g
//
LOCUS       DAOSIF010000562         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51346_length_1929_cov_3.660619, whole genome shotgun sequence.
ACCESSION   DAOSIF010000562 DAOSIF010000000
VERSION     DAOSIF010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51346_length_1929_cov_3.660619"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1318
                     /locus_tag="PLT35_13850"
     CDS             209..1318
                     /locus_tag="PLT35_13850"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HOG30355.1"
                     /translation="MGGARWSSCIPLIGILLVLAGPRAESDAREAQPRIINVADDFIA
                     YYKACVNAGAETRAEQWDRMLESKHQAFFDHAIYRRKAGKDREEFKRELRRAFWSEVA
                     PRMQSIERLNRTAEADIRGVLAAFRKPFPGFDPARDYYITIAFSFRGKVVDVAGKDVF
                     AIGLESLSDDPLQFTITVAHELFHLYHFQFFSTSGAIYRKIWAEGMAVYASAVVVPGY
                     RDSRYLGFSVEKMNRCHELLPKMAAELRRHMGERDPALERVYLGAEENRTGIPPEAGY
                     YVGLRIVASLARDTPIDELARLKSDRVYALVEAELRKLEAGGAPTAGAAAERRTPAAG
                     RSLRRDLSNRRPRAPGVAREGADGPTNSPGGAAIS"
     gene            complement(1308..>1929)
                     /locus_tag="PLT35_13855"
     CDS             complement(1308..>1929)
                     /locus_tag="PLT35_13855"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOG30356.1"
                     /translation="DLEQLQRRILQHISEMGFFEKLWRQNKAVVVVAVILLIAILVVL
                     YMSFKPVSAAQGKPAASVPSAPVPPGSAGSAAPAGAMPAPAPPAVPSDKTSIEPSDGT
                     LFEGDIRGSVTVIVKGQEQGTYPITTMPMVLGRDAASSTVVIQNDLVSKNHLKVYPKD
                     DQFYIVDLGSTNGTYVNGERITEQLVKPEDSVQLGKKGEVKLVFKK"
BASE COUNT          314 a          624 c          675 g          316 t
ORIGIN      
        1 ttctgcggcg ccgcggcggg ttctcccgga ttcagccggg gcggcaggcg cggtccggga
       61 tggcagggaa cgcgtccggc cgcagccatt tcgcgtaaga tgcgtggtgc ccggcgccgt
      121 caaccgcggc ggcggcgcgg gcggatgcgc aggccggtgc cgtctgggcg cggacgcggc
      181 cgcaccacga gctgaggcag gaggcatcat gggaggcgcg agatggtcgt cttgcatccc
      241 gctcatcggg atcttgctcg tcttggccgg gccgcgcgcg gagtcagacg cccgcgaggc
      301 gcagccgcgc atcatcaacg tcgccgacga cttcatcgct tactacaagg cctgcgtcaa
      361 tgccggcgcc gaaacccggg cggaacagtg ggatcggatg ctcgaatcca agcatcaggc
      421 gttcttcgac catgccatct accgcaggaa ggcaggcaag gatcgcgagg agttcaagag
      481 ggagctcagg cgcgcgttct ggagcgaggt tgcgccgagg atgcagagca tcgagcggct
      541 gaacaggacc gctgaggccg acatcagagg cgtgctggcc gccttcagga agccgtttcc
      601 cggcttcgat cccgcgcgcg actactacat caccatcgcc ttcagcttca gaggcaaggt
      661 cgtcgatgtc gccggcaagg acgtcttcgc catcgggctt gagagcctca gcgacgaccc
      721 gctgcagttc acgattaccg tcgcccacga gctctttcat ctgtaccact tccagttctt
      781 ctcgacgagc ggcgccatct accggaagat ctgggctgag ggcatggctg tctacgcgtc
      841 ggccgtcgtg gtccccggct acagggacag ccggtacctg gggttttccg tcgagaagat
      901 gaaccggtgc cacgaactgc tgccgaagat ggcggctgag ctgcgcaggc acatgggcga
      961 gcgcgacccc gctctcgagc gggtctatct cggcgccgag gagaacagga ccgggattcc
     1021 gccggaagcg ggatactacg tcggactgcg gatcgtggcc tcgctcgccc gggacacgcc
     1081 gattgacgag ttggcgagac tcaagagcga cagggtctac gcccttgtgg aggccgagct
     1141 gaggaagctg gaggcggggg gcgcccccac ggcaggggcg gcggccgagc gccggacgcc
     1201 cgccgccggg cggagcctcc ggcgcgacct ctcgaaccgc cgcccccgcg cgccgggcgt
     1261 cgcgcgcgag ggggcggacg gtccgacgaa ctcgcccggc ggcgccgcta tttcttgaat
     1321 accagcttca cctcgccctt cttgcccagc tggaccgagt cctccggctt gaccagctgc
     1381 tccgtgatcc gctcgccgtt gacatacgtc ccgttcgtgg atcccaggtc gacgatgtag
     1441 aactggtcgt ctttcgggta caccttcagg tggttcttcg agaccaggtc gttctggatg
     1501 acgacggtgc tggaggcggc gtcgcggccg agcaccatgg gcatggtcgt aatcgggtag
     1561 gtcccctgct cctggccctt gacgatcacc gtcaccgacc cgcggatgtc cccctcgaag
     1621 agagtgccgt cactgggctc gatcgacgtc ttgtcgctgg gaaccgcggg cggtgccggc
     1681 gccggcatgg cgccggcggg tgccgcggat cccgcggatc ccggcggtac gggcgcggag
     1741 ggcaccgacg cggcaggctt gccttgagct gccgacacgg gcttgaacga catgtagagc
     1801 acgacgagaa tcgcaatcag caggatgacg gcgacaacga cgacggcctt gttctggcgc
     1861 cacagttttt cgaagaagcc catctcgctg atgtgctgca ggatccggcg ctgcagctgc
     1921 tcgagatcg
//
LOCUS       DAOSIF010000563         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51386_length_1928_cov_2.948211, whole genome shotgun sequence.
ACCESSION   DAOSIF010000563 DAOSIF010000000
VERSION     DAOSIF010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51386_length_1928_cov_2.948211"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..483)
                     /locus_tag="PLT35_13860"
     CDS             complement(241..483)
                     /locus_tag="PLT35_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30357.1"
                     /translation="MTTLPADALADLLHAALTYRPIYVAGLVADLLDAGVPEATAPAL
                     RELRAACLARFSGRTASRDRARLATAIEGLRKAVEA"
     gene            complement(530..742)
                     /locus_tag="PLT35_13865"
     CDS             complement(530..742)
                     /locus_tag="PLT35_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30358.1"
                     /translation="MNKLPGDWTTRGTVIESRRSVRIPPREVSLATLTAEVRAMRRDG
                     LVLTSECGPTADGWYLIVAVHPSLIA"
     gene            1235..1465
                     /locus_tag="PLT35_13870"
     CDS             1235..1465
                     /locus_tag="PLT35_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30359.1"
                     /translation="MTIREISATYSAKIDADDIAAIPNADDRDYVKRHASFKGIMPTI
                     TDFCARWHVHATVREGRKIIARVGRTGNITYR"
     gene            1473..1736
                     /locus_tag="PLT35_13875"
     CDS             1473..1736
                     /locus_tag="PLT35_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30360.1"
                     /translation="MSNAASLRSALLVAAAAMDATPTWAPDYAAKVATWREAVAALAA
                     ARTVATPERTDLYRGTYADACRHFGGPARFVCGDVAPKTWRRA"
BASE COUNT          281 a          707 c          681 g          259 t
ORIGIN      
        1 cttgagcttg atctccatgg agcctcctga cgccgaaggc cggccacccc ggagggcggt
       61 cggctggcgc tgcggcagcg tgggtcaggc gggcccgcgc ctcgccggag ttgcggcagg
      121 cgacgcgtcc gcggaagtct gcgaggcagc ccccgtgctc tcggtgccac gccgccgcat
      181 gctcggcggt ccagagcgag ccgcagatcg cctcttcgcc gcagacgcag cggagcacgc
      241 tcacgcctcc accgccttgc gcagcccctc gatcgccgtc gcgaggcgcg cacggtcccg
      301 gctcgccgtg cggcccgaga agcgcgcgag gcacgccgcg cgaagctcgc gcagggccgg
      361 cgccgtcgcc tcgggcacgc ccgcgtcaag caagtcggcg acgaggccgg cgacgtagat
      421 cgggcggtag gtgagcgccg cgtggagcag gtcggcgagg gcgtcggcgg ggagcgtcgt
      481 catgtccgat cgtagttgca cgcgtcgtgc cactgttgca gcgtaacggt cacgcgatca
      541 gcgacggatg cacggcaacg atcagatacc agccgtccgc cgtggggccg cactcggacg
      601 tgagcacgag gccgtcgcgt cgcatcgcgc gcacctcagc ggtaagcgtc gcaagcgaaa
      661 cctcgcgcgg cgggatacga accgagcggc gagactcgat cacggttccg cgggtcgtcc
      721 agtcgccggg gagcttgttc atgctctccc ctattgcacc cgtcgtgcca ctttgcgatc
      781 actaaaacaa ggccggacgc acccgcgctc gctaagttgt tacgctgtta cgcgcgtaac
      841 tgtgacgctg ttacggcgtc acacgcgaac gatgttacac gctcgactgg cgcgacgtgt
      901 gcgcagcacc gcgaagccga ttacgcgcct cgcgcatcct ttgcgcgttt ttggcccgcc
      961 gcgcctcgtc gcctgcccca tatcgggccg cgctcgcccg cgtggccgcc tccgtctgcg
     1021 agggccacct gtgcgctgcc gcaagctgcg cgagctcgtc accgtgcccc gcgaggcgcc
     1081 tgtagagggt gcgcaggggg atgctgagcg cagccgcggc gcggacgcgc gagccgttgc
     1141 aggtgatgag ggcggcgagg atgacggggt ccacggaagg accgggtgca cgcacggtgc
     1201 cgcgtgtggc acgacgggtg caacacaggg aggcatgacg attcgagaaa tcagcgccac
     1261 ctactccgcc aagatcgacg ccgacgacat cgccgccatc cccaacgccg acgatcgcga
     1321 ctacgtgaag cgtcacgcgt cgttcaaggg catcatgccc acgatcaccg acttctgcgc
     1381 ccgctggcac gtgcacgcga ccgttcgcga gggccgcaag atcatcgccc gcgtgggccg
     1441 gaccggcaac atcacgtacc gctgaggacg ccatgagcaa cgccgcctcc ctccgctccg
     1501 cgctcctcgt cgccgcagcc gccatggacg cgaccccgac gtgggcgccc gactacgccg
     1561 ccaaggtggc cacgtggcgc gaggcggtcg ccgcgcttgc tgcggcgcgg actgtcgcca
     1621 ccccggagcg caccgatctc taccgcggca cctacgcgga cgcgtgccgc cacttcggcg
     1681 gcccggctcg gttcgtgtgc ggggacgtgg cgcccaagac gtggcgccgc gcctgaccct
     1741 ctcacccgcc cgcgcccggc ccgccctcgt cctcacctct gggcgcgcag cagcctccgt
     1801 agcggcggcg gtgcgggacg gccagccaag ggccgcggca aggcgcgcga gctcgtcggc
     1861 gtgcccttcc aggcgccggg cgagcgtgcg cagggagagg ccgagcgcag ccgcggcgtg
     1921 ggcgcggt
//
LOCUS       DAOSIF010000564         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51531_length_1924_cov_2.632959, whole genome shotgun sequence.
ACCESSION   DAOSIF010000564 DAOSIF010000000
VERSION     DAOSIF010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51531_length_1924_cov_2.632959"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PLT35_13880"
     CDS             <1..1466
                     /locus_tag="PLT35_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30361.1"
                     /translation="ILARAQGHADTVKAQADAVLTAAQTRAQQLTEQAEALHREAAAL
                     HAAAVTDRDQAAALLATAHDDASALVRDAAAQASLVADAAARSSQAEIDEARREADEL
                     RRAAQAKADEIVAAAQADYDGAMARFAAHEAARTSLLDERHTAIATEESTRLAMAATS
                     ANALVTAATADAERMRAHAREEAELTQAAAEALRARTVAEAADVRRAAAAEVAALQAQ
                     ARAEADAMLSAAQTHLAWAERTMGEIIDGANAEAERTRVVTARTLADHARRRRQQIQG
                     VLARVSHTSRQRLDEASAESARQRAQAQAILEAAAADAKRTAADAGREAARLVADAEA
                     MATATVERAELRLAEADHGARLIREGTAAEVEKLHREAHADRRAAREELVRTLAQARE
                     EADRLRAQAREAVDAARAEVHTLAARRDDINTQLGHLSNVIEALAVPERLVGDAALPA
                     PLQAPLAEGEHRPVLDRVNTAASACQADDHSLVEPLP"
     gene            1500..>1924
                     /locus_tag="PLT35_13885"
     CDS             1500..>1924
                     /locus_tag="PLT35_13885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOG30362.1"
                     /translation="MTEHTQFRAVLRGYDPAQVDQVVAELTAAAEAARQEAAGLSVQA
                     SKLEAGHAELRSQLDARDAQLTKLREASRTAAAPSFAGLGDRVGQILTLADEEAAALR
                     AASAAEAAELKASTESAAAAIRSDADRYAEDVRTKADADA"
BASE COUNT          322 a          715 c          675 g          212 t
ORIGIN      
        1 agatcctcgc ccgcgcccag ggccacgccg acaccgtcaa ggcgcaggcc gacgctgtgc
       61 tgacggctgc ccagacgcgc gcacagcagc tgaccgagca ggcggaagcg ctccatcggg
      121 aggcggcggc actgcacgcg gccgccgtca cggaccgcga tcaggcggcc gccctgctgg
      181 caacggcgca cgatgacgcc tcggcactag tgcgcgacgc ggctgcccag gcgtcgctcg
      241 tcgccgacgc cgcagcacgc agtagccagg ccgaaatcga cgaggcccgc cgcgaagccg
      301 acgaactgcg ccgggccgca caggcgaagg ctgacgagat cgtggcggcc gcccaggccg
      361 actacgacgg cgcgatggcc cgcttcgccg cgcacgaggc tgcccggacg tcgctgctgg
      421 acgagcggca taccgctatc gcgacagagg agtcgacccg gctcgcgatg gcagcgacca
      481 gcgcgaacgc cctggtgacc gctgcgaccg ccgatgcaga gcgcatgcgg gcgcatgcgc
      541 gcgaagaggc cgagctcacg caggctgccg cagaggcgct gcgggcccgg accgttgccg
      601 aggccgctga cgtgcggcgt gctgcggccg ccgaagtcgc ggcgttgcag gcccaagcac
      661 gagccgaggc cgacgcgatg ctcagcgccg cgcagaccca cctggcgtgg gccgaacgca
      721 cgatgggcga gatcatcgac ggggccaacg cggaggccga gcgcacccgt gtcgtgaccg
      781 ccaggacgtt ggccgaccac gcgcgccggc gccggcagca gatccaggga gtgctggccc
      841 gggtctcgca cacctcgcga caacgtctcg acgaggcttc ggccgagtct gctcgccagc
      901 gggcccaagc tcaagcaatc ctcgaggccg cagcagccga cgccaagcgc accgccgccg
      961 acgccggtcg agaagcggcc cggctcgtcg ccgacgccga ggctatggcc acagcgacgg
     1021 ttgaacgtgc cgagctgcgc cttgccgagg ccgatcacgg tgcccggctc atccgcgaag
     1081 gcacggccgc agaggtggag aagctgcatc gcgaggccca cgccgaccgc cgggcggccc
     1141 gcgaggaact cgtccggacc ctcgcgcagg cacgcgagga ggccgaccga ttgcgcgctc
     1201 aagcacgcga ggccgtcgac gcggcccgtg ccgaggtgca cacgctggca gctcgccggg
     1261 acgacatcaa cactcagctc ggccatctct ccaacgtcat cgaagccctc gccgtgccgg
     1321 agcgactcgt cggcgatgca gcgctgcctg ccccgctcca ggcaccactg gccgagggcg
     1381 aacaccgacc cgtccttgat cgcgtcaaca cggccgcttc cgcgtgccag gctgacgacc
     1441 attcgctcgt tgaacctctc ccctgaccga cctacgccct ctgaatcgat gggatctcga
     1501 tgaccgagca cactcagttc cgcgccgtcc ttcgtggcta cgaccccgca caggtcgacc
     1561 aagtggttgc cgagctcacg gctgctgctg aagccgcgcg ccaggaggcg gccggactca
     1621 gcgtccaggc cagcaagctc gaggccggac acgccgagct gcgcagccag ctagacgcgc
     1681 gcgacgccca gctgacgaag cttcgggaag ccagccggac cgcggccgcg ccgagtttcg
     1741 ccggtctggg tgaccgggtg ggtcagatcc tcaccctcgc tgacgaggag gccgcggcgt
     1801 tgcgggcggc gtcagcggcc gaggctgccg agctcaaggc gtcgacggag agtgccgccg
     1861 ccgcgattcg cagcgacgcc gatcgttatg ccgaggatgt tcgcacgaag gccgacgccg
     1921 acgc
//
LOCUS       DAOSIF010000565         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51613_length_1922_cov_3.562935, whole genome shotgun sequence.
ACCESSION   DAOSIF010000565 DAOSIF010000000
VERSION     DAOSIF010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51613_length_1922_cov_3.562935"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1922
                     /locus_tag="PLT35_13890"
     CDS             <1..>1922
                     /locus_tag="PLT35_13890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOG30363.1"
                     /translation="TLVARGGDLPGRTLRFHLDAVSRGAPVDADGRPVPVSAIVRIES
                     GDGEDLDRVRLDAPLLTTLITTSRQKRRRVALSLIPPHMAHAILAIEDRRFYRHPGID
                     PIRIAGAIRTNLSGKHPYLEGASTITQQLVKNFFLTPEKSYRRKIQEQLLAMVLETRA
                     SKDEILELYLNEVYLGQRGSYAVHGVAQAARMFFGKDVTNLTLAEAATIAGVIQTPYR
                     HSPFSSPDRSRERRNVVLKAMADEGFVTREAAERASREPLQTVARALDTEAPYFVDLL
                     GQVLADDYPGMLEGPEGVDIHATLDVNLQRAAQDAVRAGMVQVDAQLARKGVKALAQV
                     ALIAVEPRTGEILAYVGGRSYNQSQFNRAVSARRQPGSVFKPFVYLAAFEAAAQEGRT
                     DLTPATVVADEQTTFAYDDQTWTPRNYEDEYDGVITLRRALANSRNVATVRVAESVGF
                     DTVAALWRRVGTSTAPRAYPSIALGVFEATPLEIVKAYSIFPNLGETRPPKAIIGLER
                     RGAAVPLREAQPVRVARADTTYLVTSLMRSVINEGTGASARAMGFTHDAAGKTGTTND
                     LRDAWFVGFTPDLLTAVWVGFDDNQPLGMSGSAAALPIWTRFMRAALGGRPNLAFQAP
                     AGIAFADIDRDTGALA"
BASE COUNT          303 a          696 c          649 g          274 t
ORIGIN      
        1 tcacactggt ggcacggggc ggcgacctgc ccgggcggac gctcaggttc caccttgatg
       61 ccgtcagccg cggcgccccg gtggacgcgg atggccggcc ggtccccgtc tctgccatcg
      121 tccgaatcga gtccggggac ggcgaggatc tcgatcgggt ccggctcgac gcgcccctcc
      181 tgacgacgct catcacgacc agccgccaga agcgcagacg ggtcgccctc tcgctcatcc
      241 cgccgcacat ggcgcacgcg atcctggcca tcgaggatcg gcgtttctac cgccatcccg
      301 gcatcgatcc gatccggatt gcgggcgcca tccgcacgaa cctcagcgga aagcacccgt
      361 acctggaagg cgcgagcacg attacgcagc agctcgtgaa gaatttcttc ctcaccccgg
      421 agaagtccta ccggcgcaag atccaggagc agctgctggc aatggtgctc gagacgcgcg
      481 cctcgaagga cgagatcctc gaactgtacc tgaacgaggt ctacctcggg cagcgcggat
      541 cgtacgcggt gcacggcgtc gcccaggcgg cgcggatgtt cttcggcaag gacgtcacga
      601 acctcacgct ggctgaagcc gcgaccatcg ccggcgtcat tcagacgccg taccgtcact
      661 cgccgttctc gtcgccggac cgctcccgcg agcggcgcaa cgtggtgctg aaggcgatgg
      721 ccgacgaggg cttcgtcacg cgggaggccg ccgagcgggc cagccgggag ccgctgcaga
      781 ccgtcgcgcg cgcgctcgac acggaggccc cctatttcgt cgatctcctc ggccaggtgc
      841 tggccgacga ctaccccggc atgctcgaag gccccgaggg cgtggacatc cacgccacgc
      901 tcgacgtgaa cctgcagcgc gccgcgcagg acgcggtgcg ggcgggcatg gtgcaggtcg
      961 acgcgcagct ggccaggaag ggcgtgaaag cccttgcgca ggtcgcgctg atcgccgtcg
     1021 agccccgcac cggcgagatc ctcgcctatg tcggagggcg ctcgtacaac cagtcgcagt
     1081 tcaaccgggc cgtcagcgca cgacggcagc cgggctcggt gttcaagcct ttcgtctacc
     1141 tcgccgcctt tgaagccgcc gcgcaggaag gccgcacgga tctcacgccg gccaccgtcg
     1201 tggccgacga gcagacgacg ttcgcctacg acgaccagac gtggacgccg aggaactacg
     1261 aggacgagta cgacggcgtc atcaccctgc gccgggccct ggcgaactcg cggaacgtgg
     1321 ccaccgtgcg cgtggccgag tcggtgggct tcgacaccgt ggcggcgctc tggcggcgcg
     1381 tcgggaccag cacggcgccg agggcgtacc cgtcgatcgc cctgggggtc ttcgaggcca
     1441 cgcctctgga gatcgtcaag gcctactcga ttttcccgaa tctcggcgag acccgcccgc
     1501 cgaaggccat catcggcctg gagcgccgcg gagcggccgt cccgcttcgc gaggcgcagc
     1561 cggtccgcgt ggcccgcgcc gacacgacct atctcgtgac cagcctgatg cggagcgtga
     1621 tcaacgaggg caccggcgcc agcgcgcgcg ccatgggctt cacccacgac gccgccggca
     1681 agaccggcac gacgaacgat ctgcgcgacg cctggttcgt cgggttcact cccgacctcc
     1741 tgacggcggt ctgggtgggc ttcgacgaca accagccgct gggaatgagc ggctcggctg
     1801 cggcccttcc catctggacc cggttcatgc gggcggccct cggcggccgt cccaacctgg
     1861 cgttccaggc tcccgcggga atcgcgtttg ccgacatcga tcgcgacacg ggcgccctgg
     1921 cg
//
LOCUS       DAOSIF010000566         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51656_length_1921_cov_3.182744, whole genome shotgun sequence.
ACCESSION   DAOSIF010000566 DAOSIF010000000
VERSION     DAOSIF010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51656_length_1921_cov_3.182744"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLT35_13895"
     CDS             <1..635
                     /locus_tag="PLT35_13895"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG30364.1"
                     /translation="VGEPLRRVDGRPFQQMLRDEICRPLGILNDLFVGIPDEVEPRVA
                     VLEEIFESGKEPVVDDSKPQSVPGFMQPLYRMMNHPIARRTCNPASNGIMTARAIARH
                     YAALLPGGVDGVELLPTERILAAVQRQLPKRLPENGELPNFALGYRLEGPQDPAGRRL
                     PTFGHGGYGGSTGTADLENRLAVGFTKNLFSKNDARESVLRELRSVLGIS"
     gene            complement(652..1656)
                     /gene="sppA"
                     /locus_tag="PLT35_13900"
     CDS             complement(652..1656)
                     /gene="sppA"
                     /locus_tag="PLT35_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOG30365.1"
                     /translation="MKTTRFLLAACGAAILCGCGQDVGWVIKPVPLDETLRETVIAAD
                     PGLFVSDKIAVLDVDGLLMNRRATGWMMPGENPVSLFVEKLDRAESDPNVRAVVVRIN
                     SPGGAVTASDVMYRRLVRFRAARPGVPVVAVIEDVGASGGYYVACGADTILAHPTSVT
                     GSIGVIVQTVSFAGTMRMIGIDAKAVTSGARKDMASPLKPLDEKDLAILQGIVDQYYR
                     RFLQVVESARPNLKPDQVRALADGRVYTGEQAKDNGLVDGLGYVDDAVLMAKKRSARA
                     RVKLVMYHRPLGYRANVYSAAPNLAPQVNLLNIDLPGVLSLAQPEFLYLWTGRATGSP
                     "
     gene            complement(1710..>1921)
                     /locus_tag="PLT35_13905"
     CDS             complement(1710..>1921)
                     /locus_tag="PLT35_13905"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOG30366.1"
                     /translation="SAIRYVAQTDRIAPARMIAVGDDVNDLPMIRGAGLGVAMPGAPE
                     TVRAEADRVAEGGLAKFLHDLLAGA"
BASE COUNT          297 a          652 c          685 g          287 t
ORIGIN      
        1 tggtcggaga accgctgcgg cgagtggatg gcaggccatt ccagcagatg ttgcgggacg
       61 aaatctgccg tccgctgggg atcctcaacg acctgttcgt gggcattccc gacgaggtcg
      121 agccgcgcgt cgccgtcctg gaggaaatct tcgaatcggg caaggagcca gtcgtggacg
      181 acagcaagcc gcagagcgtc cccggcttca tgcagccgct gtatcggatg atgaatcacc
      241 cgatcgctcg gcgcacgtgc aatccggcgt ccaacggaat catgaccgcc cgcgcgatcg
      301 cgcggcatta cgccgccctg ctgcccgggg gggtggacgg cgtggaactg ctgccgacgg
      361 agcgaatcct cgcggcggtg cagcggcaac tgcccaaacg cctgccggaa aacggcgagc
      421 tgccgaactt cgccctgggc taccgtctcg aaggaccgca ggacccggcc gggcggcggc
      481 taccgacctt cgggcacggc gggtacggcg gatccaccgg aaccgccgac ctggagaacc
      541 gcctggccgt cggattcacc aagaacctct tctccaaaaa cgacgcgagg gaatccgtcc
      601 tccgcgaact gcggagcgtc ctggggattt cgtgaacgag tctctccccg cttacggtga
      661 gccggtggcg cggccggtcc agaggtagag gaactccggc tgggccagcg agagcacgcc
      721 cggcaggtcg atgttcagca ggttgacctg gggcgcgagg ttcggcgcgg ccgagtagac
      781 gttggcgcga tagcccagcg ggcggtggta catgacgagc ttgacgcgcg cccgggcgct
      841 gcgcttcttg gccatcagga ccgcgtcgtc gacgtatccc aggccgtcca ccagcccgtt
      901 gtccttcgcc tgctcgccgg tgtagacgcg cccgtcggcc agcgcgcgga cctggtcggg
      961 cttgaggttc ggccgggccg actcgaccac ctggaggaac cgccggtagt actggtccac
     1021 gatgccctgg aggatggcga ggtctttctc gtcgaggggc ttgagcgggc tggccatgtc
     1081 cttgcgcgcg ccggaggtga cggccttggc gtcgatgccg atcatccgca tcgtcccggc
     1141 gaaactgacg gtctggacga tcaccccgat cgagccggtg acgctggtgg gatgggccag
     1201 gatcgtgtcg gccccgcagg cgacgtagta tccgccgctg gcgccgacgt cctcgatgac
     1261 ggcgacgacg ggtacgccgg gcctggccgc ccggaagcgc accaggcgcc ggtacatcac
     1321 gtcgctggcg gtgaccgccc cgccgggcga gttgatgcga accaccaccg cgcggacgtt
     1381 cgggtcgctc tccgcgcggt cgagcttctc cacgaacagc gagacggggt tctcgcccgg
     1441 catcatccag ccggtcgcgc ggcgattcat cagcagaccg tccacgtcga ggacggcgat
     1501 cttgtcggac acgaacagcc ccgggtcggc cgcgatgacc gtctcgcgga gcgtctcgtc
     1561 gaggggaacg ggcttgatga cccagccgac gtcctgcccg cacccgcaca ggatcgccgc
     1621 gccgcacgcc gccaggagga accgtgttgt tttcatggcg gggatggtag cgcgcggggg
     1681 cgcgggcgtc aaccacggca gtccgctcgt cacgcgccgg cgagcaggtc gtggaggaac
     1741 ttcgccagcc cgccctcggc gacgcggtcg gcctcggccc ggactgtctc cggcgcgccg
     1801 ggcatggcca cccccagccc cgcgccgcgg atcatcggaa ggtcgttgac gtcgtcgccg
     1861 acggcgatca tccgcgccgg ggcgatccgg tcggtctggg cgacgtagcg gatggccgac
     1921 c
//
LOCUS       DAOSIF010000567         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51831_length_1917_cov_2.765306, whole genome shotgun sequence.
ACCESSION   DAOSIF010000567 DAOSIF010000000
VERSION     DAOSIF010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51831_length_1917_cov_2.765306"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1455)
                     /locus_tag="PLT35_13910"
     CDS             complement(169..1455)
                     /locus_tag="PLT35_13910"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOG30367.1"
                     /translation="MTTPATITHGDKSLELPFVEATQGNNGYDIGKLMAATGDTTFDI
                     GFANTAVCKSAITFIDGDAGILRYRGYPIDQLAEKSSFLETAYLVLHGELPTAEQLTE
                     FTHKISHHMVIDERIREMFRIFPRRSHPMPVLSVGVTALGLFSPETIGFDDEHLDLAT
                     VKLLGKVPTLAAYGYKNSIGMPTLFPDYSLSYVENFLRMSFGAVTQPYEFDDEVTKAL
                     DVLLILHADHEQNCSTSTVRLVGSAQTNIYQSISSGINALNGPLHGGANQAVLEMLDD
                     IADRGISVKQYVDRVKDKNHNERLMGFGHRVYKNYDPRAAIIKKHADSILRKHGGNDK
                     LLDIALQLEEAALSDDYFISRKLYPNVDFYTGLIYRAMGFPTDMFTVLFAIGRLPGWI
                     AQWREMIKDPATKIGRPRQIYIGEPERAYVPMANRK"
     gene            1738..>1917
                     /locus_tag="PLT35_13915"
     CDS             1738..>1917
                     /locus_tag="PLT35_13915"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOG30368.1"
                     /translation="MSTIRDLQGTYALDASHSSLAFIARHAMVTKVRGSFSAFTGEAT
                     INGAEPAASSLKVVVD"
BASE COUNT          301 a          577 c          676 g          363 t
ORIGIN      
        1 tcccggacgc tgccgctcgc gtccctcgtt cgggacgccg cagcgcaggc gtcccgggtg
       61 ctgcgtccgg cggggcagcg cccccagccg gccggacgcg tccggcgaga acggtcgtga
      121 caaagcaaaa cgcccgccgg aggaccggcg ggcgatgctg acccgcggct acttgcggtt
      181 ggccatcggg acgtaggcgc gctcgggctc gccgatgtag atctgacgcg ggcgtccgat
      241 cttggtggcc gggtccttga tcatctcgcg ccactgggcg atccagccgg ggaggcgtcc
      301 gatggcgaac agcaccgtga acatgtcggt ggggaagccc atcgcgcgat agatgaggcc
      361 cgtgtagaag tcgacgttcg ggtacagctt gcgggagatg aagtagtcgt cggacagcgc
      421 cgcctcctcg agctggaggg cgatgtcgag gagcttgtcg ttgcccccgt gcttgcggag
      481 gatggagtcc gcgtgcttct tgatgatcgc cgcgcgcggg tcgtagttct tgtagacccg
      541 gtgcccgaag cccatgaggc gctcgttgtg gttcttgtcc ttgacgcggt cgacgtactg
      601 cttgacggag atgccgcggt cggcgatgtc gtccagcatc tcgaggacgg cctggttggc
      661 gccgccgtgg agcggtccgt tgagggcgtt gatgcccgac gagatcgact ggtagatgtt
      721 cgtctgtgcc gagccgacga ggcggacggt cgacgtggag cagttctgct cgtgatcggc
      781 gtgcaggatc agcaggacgt ccagcgcctt ggtgacctcg tcgtcgaact cgtacggctg
      841 cgtgaccgcg ccgaacgaca tgcgcaggaa gttctcgacg tagctcagcg agtagtcggg
      901 gaacagcgtc ggcatgccga tggagttctt gtagccgtac gccgcgagcg tcggtacctt
      961 gccgagcagc ttcaccgtgg cgaggtcgag gtgctcgtcg tcaaagccga tggtctcggg
     1021 ggagaacagg cccagcgccg tcacgccgac gctcaggacg ggcatcgggt gggagcggcg
     1081 cgggaagatg cggaacatct cgcggatgcg ctcgtcgatg accatgtggt ggctgatctt
     1141 gtgggtgaac tccgtcagct gctctgccgt cgggagttcg ccgtggagca ccaggtaagc
     1201 ggtctccagg aagctcgact tctcggcgag ttggtcgatc gggtagccgc ggtagcggag
     1261 gatgccggcg tccccgtcga tgaacgtgat cgccgacttg cacaccgccg tgttcgcgaa
     1321 cccgatgtcg aaggtcgtgt cgccggtcgc ggccatcagc ttgccgatgt cgtagccgtt
     1381 gttgccctgg gtcgcctcga cgaacggcag ctcgagggac ttgtcaccgt gcgtgatcgt
     1441 ggcgggggtt gtcatagctc tcctcctcat tgaagcagcc ctgcttacgc tggtcagact
     1501 acctgcggct gcggcgccgg agcacccccg gcgaagttct cccatcatgg catcgcgatg
     1561 tgcctacgtc gagatacttg acctcaagat ggaaaggctt ttggacgccg cgcgtcgcgt
     1621 cccctggatc ggcgatcggc ccggagtgaa gcctgagccc aggtagttga cacttcaatg
     1681 aactgggcct agagtcggag ttgttgagat ttcaaccatg ctttgaggag gcacttcatg
     1741 agcaccatcc gcgatctgca gggcacttac gccctggacg cctcgcactc gtccctggcc
     1801 ttcatcgcgc gtcacgcgat ggtgaccaag gtccgcggca gcttcagcgc gttcaccggc
     1861 gaggcgacca tcaacggggc cgagcccgcg gcgtccagcc tgaaggtcgt cgtcgac
//
LOCUS       DAOSIF010000568         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51905_length_1915_cov_3.142473, whole genome shotgun sequence.
ACCESSION   DAOSIF010000568 DAOSIF010000000
VERSION     DAOSIF010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51905_length_1915_cov_3.142473"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLT35_13920"
     CDS             complement(<1..618)
                     /locus_tag="PLT35_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30369.1"
                     /translation="MSRFWSRRITVVLVDRTSGVGRRLTIPLGWTLAAIGFLFTLPVL
                     VGLGIRLGTVAEIASLRADAAALMMENRSYKAATGELATQVVSLQGVIEELSSRANLD
                     PASARAMQQLPAVVRDRAMGGEASVAERSTARSAPLSSPEDTFGMIREVLGRLESGLR
                     VVRTDVERREALAASTPSIWPAVGWLSAGFGYRPDPFTGASSFHYG"
     gene            complement(748..>1915)
                     /locus_tag="PLT35_13925"
     CDS             complement(748..>1915)
                     /locus_tag="PLT35_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOG30370.1"
                     /translation="PTGGILPFAMAAARGGVHAVMVPAANAAEASAVSGLGVWPVASL
                     LDATSLVSDLREERRYRGAAAPAAAVQAAPHPDFAEVRGQPVARRALEVAAAGGHHVL
                     LTGPPGSGKTMMARSLAGILPPLSREESLEVTSIHSVAGLLPAGSGLIAARPFRAPHH
                     TISDVALVGGGAHPRPGELSLAHGGVLFLDELPEFERRALEALRQPLEEGRVRIARAA
                     ATVEFPALVTLVGAMNPCPCGYFGHPLRACRCAPVQRERYAARISGPLLDRIDLCVEV
                     PWVPPDVLVDERPQEGSSAMRERVTLARARQASRQPETGAATNARLTGRALRTLARPD
                     AEGRALLGAAMERLALSGRAHDRVLRVARTIADLAGDGTVGAGHVAEALQYRLG"
BASE COUNT          304 a          717 c          676 g          218 t
ORIGIN      
        1 gccgtagtga aagctggagg cgcccgtgaa gggatcaggc cgatagccga aacccgccga
       61 cagccagccc accgcgggcc agatggacgg cgtcgacgcc gccagggcct cgcgccgctc
      121 gacgtccgtc cggacaacgc gcaagccgct ctccagccgg ccgagcacct cgcggatcat
      181 gccgaaggtg tcctcgggag aagagagggg agccgagcgc gccgtcgacc gctcggccac
      241 cgaggcctcg ccgcccatcg cccggtcccg gacaacggcc ggcagctgct gcatggcccg
      301 ggccgaggcc ggatcgaggt tcgcgcggga cgacaactcc tcaatgacgc cctgcagcga
      361 cacgacctgc gtcgcaagct cgcccgtggc cgctttgtag ctccggttct ccatcatgag
      421 ggccgcggcg tccgcccgca gcgaggcaat ctccgccacg gtcccaaggc ggatacccag
      481 gccgaccagc acgggcagag tgaacagaaa gccgatggcc gcgagcgtcc atcccagcgg
      541 gattgtgagt cgccgcccga cgccgctggt gcggtcgacg agaacgaccg tgatgcgccg
      601 tgaccagaat ctggacatat tgcagaaaac cagtcggatc cgccgcatgg ggcgctggcc
      661 gactggttct gagtaggaga ctctagcaca gcgggtcgga cacccggaag cgaaaaagat
      721 ggatgagcga acatacggcg aatgcgctca cccaagccgg tactgcagcg cctccgcgac
      781 gtggccagcg ccgaccgtgc cgtcgccggc gaggtccgcg atcgttcgcg ccacgcgcag
      841 cacgcggtca tgcgcgcgtc cgctgagcgc gaggcgctcc atcgcggcgc cgagaagcgc
      901 ccgcccctcg gcatcgggcc tcgccagcgt ccggagcgcc cgccctgtca gccgcgcatt
      961 cgtggccgcg ccggtctccg gctgccgcga ggcctgccgg gcccgcgcga gggtcacgcg
     1021 ctcgcgcata gcggacgacc cctcctgcgg gcgttcgtcg accaggacgt cgggcggcac
     1081 ccacggcacc tccacgcaca ggtcgatgcg gtccaggaga ggccccgaga tccgggcggc
     1141 gtaccgctcg cgctggacgg gcgcgcatcg gcaggcgcgc aggggatgcc cgaagtagcc
     1201 gcacggacag gggttcatcg cgccaacgag cgtcaccagt gccgggaact cgacagtcgc
     1261 cgccgcccgg gcgatccgga cccgcccctc ctccagcggc tggcgcagcg cctccagggc
     1321 ccggcgctcg aactcgggca gttcgtcgag gaacaggacg ccgccgtgcg cgaggctgag
     1381 ctccccgggc cgcggatgag cgccgcctcc aaccagcgcg acgtccgaga ttgtgtggtg
     1441 gggcgcccgg aacggcctcg cggcgatcag gcccgatccc gccgggagca ggcccgcaac
     1501 cgagtggatc gaggtcacct cgagcgactc ctcgcgcgag agcggcggca ggatgcccgc
     1561 caggctgcgc gccatcatgg tcttgcccga tcccggaggc ccggtcagca ggacatggtg
     1621 gcccccggcc gcggccacct cgagcgcccg cctggcgacg ggctgtcccc tgacctccgc
     1681 gaagtccggg tgcggcgcgg cctgaaccgc tgccgccggc gcggcggccc cgcggtagcg
     1741 ccgttcttcc ctgagatccg acacgagcga cgtcgcgtcg agcagggagg ccacgggcca
     1801 cacgccaagc ccggagaccg cagacgcctc ggccgcgttt gctgctggaa ccatcaccgc
     1861 gtggacgccg ccccgtgccg cggccatcgc gaacggcagg atgccgcccg tcggg
//
LOCUS       DAOSIF010000569         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51932_length_1914_cov_4.261969, whole genome shotgun sequence.
ACCESSION   DAOSIF010000569 DAOSIF010000000
VERSION     DAOSIF010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51932_length_1914_cov_4.261969"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="PLT35_13930"
     CDS             <1..1311
                     /locus_tag="PLT35_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30371.1"
                     /translation="GECDGVQYHISWLGGAPLAPVVGREFTAPAPVVGSVVLGILLIG
                     AGGSVIKPCVSGTVQKTAGARATLGFAIFYMVINIGSLFGRGTGYVVRRGYGPATVLA
                     VVAACAAAAAALVFLVSKATEAKAQGRGARMTLPFVGVVGAAALAVAAIVGRSARAET
                     GDSLSYIFAVAASASAAAFFVVLLFYKEPPAQAGAPARPRRSVGRILLDMVLVLRNGR
                     FTLFLVLMSGFFFVYNQVYNVLPLYAKRVVEPSPAMDLYTAANPFVIVCLQLAITQYF
                     GKMRAIRSIVVGTVIIGVSMLINVYPIYTAGGVRAVAANWLPIGSVFVILTVALIAFG
                     ELFTSARMYEYIGSLAPKGQEGLFLGYANLPLALGSLAGGPAGAFIFNDVMARGATTR
                     PDGLLELVPAHNALGWIVLMAVGFLSALAMWLFNRWLERRPTSA"
     gene            1337..1876
                     /locus_tag="PLT35_13935"
     CDS             1337..1876
                     /locus_tag="PLT35_13935"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOG30372.1"
                     /translation="MEKRVGFSLRLAAALIDAAVILALAAGVGGTLGGLIGGAAGRAF
                     GGAGADAAATAAAGAFVGALVGALAATSGVTFLYSLIEAFTGASPGKMALRLKIGLED
                     GRRAPLPVCVRRWAVKYSGALFGLLGAVPLLSPLAMFATAAGLLVFLGCFLALGDRRQ
                     ALHDLAAGTAVFRTPDLRP"
BASE COUNT          215 a          686 c          691 g          322 t
ORIGIN      
        1 ggcgagtgcg acggcgtgca gtaccacatc tcgtggctcg gcggcgcccc gctggcaccg
       61 gtggtcggcc gcgagttcac cgcgccggcg cccgtcgtgg gatcggtcgt cctgggcatc
      121 ctgctcatcg gcgccggcgg ctcggtcatc aagccgtgcg tgtcggggac cgtccagaag
      181 accgccgggg cgcgcgcgac gctgggtttc gcgatcttct acatggtgat caacatcggg
      241 tcgctgttcg ggcgcgggac cggctacgtc gtgcggcgcg gctacggccc tgccaccgtc
      301 ctggccgttg tcgcggcgtg cgccgcggcg gccgcggcgc tggtgttcct cgtcagcaag
      361 gcgaccgagg ccaaggccca gggccgcggc gcccgcatga cgctgccgtt cgtcggcgtg
      421 gtcggcgccg ccgccctggc cgtcgccgcg atcgtgggcc ggagcgcgcg cgccgagacg
      481 ggcgacagcc tctcctacat cttcgccgtc gccgcgagcg cgtccgcggc ggccttcttc
      541 gtcgtgctcc tgttctacaa ggagccgccc gcgcaggctg gtgcgccagc caggccgcgg
      601 cggtccgtcg ggcgcatcct gctggacatg gtcctggtgc tcaggaacgg ccggttcacg
      661 ctgttcctgg tcctcatgag cgggttcttc ttcgtctaca accaggtcta caacgtgctg
      721 ccgctctacg ccaagcgggt ggtcgagccg agcccggcca tggacctcta caccgcggcc
      781 aaccccttcg tcatcgtctg cctgcagctg gccatcacgc agtacttcgg gaagatgagg
      841 gcgatccgct cgatcgtcgt gggcacggtg attatcggcg tctccatgct gatcaacgtg
      901 tacccgattt acaccgccgg cggcgtgagg gccgtggcgg cgaactggct gccgatcggg
      961 tcggtgttcg tcatcctcac ggtcgccctc attgcgttcg gcgagctctt cacctcggcg
     1021 cgcatgtacg agtacatcgg gtcgctggcg ccgaagggcc aggaaggcct cttcctcggc
     1081 tatgcgaacc tcccgctcgc gctcggctcg ctcgcgggcg gcccggccgg agcgttcatc
     1141 ttcaacgacg tcatggcccg cggcgccacg acccggcccg acgggctgct cgagctcgtg
     1201 cccgcccaca acgccctcgg ctggatcgtc ctgatggccg tcggattcct gtcggccctg
     1261 gcgatgtggc tgttcaacag gtggctcgag cggaggccga cgagcgcctg agcgccggct
     1321 gagagcagag gagatcatgg agaagcgcgt cgggttcagc ctgaggctcg ctgccgcgct
     1381 gatcgatgcc gccgtcatcc tcgcgctggc ggctggcgtc ggcggcaccc tcggcgggct
     1441 gattggaggc gccgccgggc gggccttcgg cggggccggc gcggacgccg cggcgacggc
     1501 cgcagccggc gccttcgtcg gcgctctcgt cggcgccctc gcggcgacca gcggcgtcac
     1561 gttcctctac agcctcatcg aggcgttcac gggcgcgtcg ccggggaaga tggcgctgcg
     1621 gctgaagatc ggactcgagg acggccggcg tgcgccgctg ccggtctgcg tgagacgctg
     1681 ggccgtcaag tactcgggcg cgctgttcgg gctgctcggc gccgtgccgc tcctcagccc
     1741 cctggcgatg tttgccacgg cggccgggct cctggtcttc ctcggctgct tcctcgcact
     1801 cggcgacagg cgccaggcgc tgcacgacct cgccgcgggg acggcggttt tccggacgcc
     1861 cgacctccgg ccctgacggc gcgacggtca gcgcgcagcc gggggccggc gacc
//
LOCUS       DAOSIF010000570         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_51948_length_1914_cov_2.874126, whole genome shotgun sequence.
ACCESSION   DAOSIF010000570 DAOSIF010000000
VERSION     DAOSIF010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51948_length_1914_cov_2.874126"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLT35_13940"
     CDS             complement(<1..219)
                     /locus_tag="PLT35_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30373.1"
                     /translation="MRRTARPGASAAVLAAGVAVWVSAAGVPAAPARPLSPHPSLGGG
                     HAGPGPRYDQGRIEFSADIGAIYFSVGAA"
     gene            complement(317..808)
                     /locus_tag="PLT35_13945"
     CDS             complement(317..808)
                     /locus_tag="PLT35_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30374.1"
                     /translation="MLSTRTRKAVPARRIGIAFLLAAAFAVVSAGAVFAQAAPAAPIR
                     TFTGDLGIYQYVVKVDRAADFEAMVAKLKDGLQKSEDPVRKAQAAGWRVFKQVESVGE
                     GRVAYMFLIDPVAKDADYTLSAILYEAFPEAQDRTSTYEIIKNGVLGSGMVNYQLIMD
                     ARK"
     gene            complement(859..>1914)
                     /gene="hemW"
                     /locus_tag="PLT35_13950"
     CDS             complement(859..>1914)
                     /gene="hemW"
                     /locus_tag="PLT35_13950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOG30375.1"
                     /translation="YLRAMVAEIEAAPGREALAPRPRADTVYLGGGTPSLLEPAEVAC
                     LLGACRDAFALLPDAEVTLEANPESAAPGRLEAFRGAGVNRLSLGVQSFQDDDLRRLG
                     RRHDAARARLAFRDARAAGFANVGLDLMLGLPSQWLDGWMASVDEAAALAPEHVSLYV
                     LELHAASPLQADIAAAGWPMPPDDDVAAMYEAAMARLERAGYRQYEISNLAKPGFESR
                     HNLKYWRDCEWLGFGPGAHSTLGGARWRNPAGIAEWASRAEAGEPPGVARHLLTPDER
                     WTEALITALRLADGVDLADFSRRYGVDLEARADSDLQALAREGLVAREGRRLRLTRKG
                     MLVANGILRVLIAPAVR"
BASE COUNT          287 a          706 c          673 g          248 t
ORIGIN      
        1 agcagcgccg acggagaagt agatggcgcc gatgtcggcg ctgaactcga tgcggccctg
       61 gtcgtaccgg ggccccggcc cagcgtggcc gccaccgagg gacgggtgcg gggacaacgg
      121 ccgggccggc gcggcgggca cgcccgccgc cgatacccag accgccacgc cggcggccag
      181 caccgcagcc gacgcgcccg gacgtgccgt tcgacgcatg gcggaaccgc cgcgctgaga
      241 cggggtgacg cgggatgcgg cgccgcgccg cccgcccacc acaggacgac agcggaccgc
      301 gcgccggctg gcgcgcctac ttcctagcgt ccatgatcaa ctggtagttg accatgccgc
      361 tgcccagaac gccgttcttg atgatctcgt acgtggatgt ccggtcctgg gcctccggga
      421 aggcctcgta caggatcgcg gacagcgtgt aatcggcgtc cttcgcgacg gggtcgatca
      481 ggaacatgta ggcgacccgg ccctctccga ccgattccac ctgcttgaag acccgccagc
      541 cggcggcctg cgccttccgc acgggatcct cgctcttctg caggccatcc ttgagcttcg
      601 cgaccatcgc ctcgaagtcg gcggcccggt cgaccttgac gacgtactga tagatgccaa
      661 ggtcccccgt gaaggtgcgt atcggggcgg ccggcgctgc ctgagcgaac accgcgcccg
      721 cggaaaccac tgcgaaggcg gcagcgagca agaaagcgat gccgatgcgc cgcgccggaa
      781 cggccttcct ggtcctggtc gagagcatgc ctgagcctcc ttgccgatgg gcgtgccagc
      841 cccaccggtg actcaactct agcgtaccgc gggcgcaatg agaacccgga gaatgccgtt
      901 ggcgaccagc atgcctttcc gcgtcagccg caggcggcgg ccctcccgcg cgacgagacc
      961 ctcgcgggcg agggcctgca ggtcggaatc cgcgcgcgcc tcgaggtcca cgccgtaccg
     1021 gcggctgaag tccgcaaggt ccacgccgtc ggcgaggcgg agcgcggtga tgagtgcctc
     1081 cgtccagcgc tcatccggcg tcagcaggtg ccgtgcgacg cccggcggct cgccggcttc
     1141 ggcccgcgac gcccactcgg cgatgccggc ggggttcctc cacctcgcgc cgccgagggt
     1201 cgagtgcgcg cccggcccga acccgagcca ttcacagtcg cgccagtact tcaggttgtg
     1261 gcgcgactcg aaccctggct tggcgaggtt cgagatctcg tactgccggt atccggcgcg
     1321 ctccaggcgg gccatcgccg cctcgtacat cgcagccacg tcgtcgtccg ggggcatcgg
     1381 ccagccggca gccgcgatgt cagcctgcag cggcgaggcg gcgtgaagtt cgaggacata
     1441 gagcgagacg tgctccggcg ccagtgctgc ggcctcgtcg accgacgcca tccagccgtc
     1501 gagccactgc gacggcagcc ccagcatgag gtcgaggccg acgttcgcaa atcctgctgc
     1561 gcgggcatcg cggaaggcca gccgcgcccg ggccgcgtcg tgccgccggc ccagccgccg
     1621 caggtcgtcg tcctggaacg actgcacgcc gaggctgaga cgattgacgc cggcgccccg
     1681 gaaggcctcg aggcggcccg gcgccgctga ctcggggttg gcctcgagcg tcacctcggc
     1741 gtccggcagg agcgcgaacg cgtcgcggca agcgcccagg agacaagcga cctcggcagg
     1801 ctcgagcagc gagggcgtgc ccccgcccag gtagaccgtg tccgcccgcg ggcgcggcgc
     1861 gagcgcctcc cgcccgggag ccgcctcgat ctcggcaacc atcgcccgca ggta
//
LOCUS       DAOSIF010000571         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_52040_length_1911_cov_4.777478, whole genome shotgun sequence.
ACCESSION   DAOSIF010000571 DAOSIF010000000
VERSION     DAOSIF010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52040_length_1911_cov_4.777478"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /gene="gpmA"
                     /locus_tag="PLT35_13955"
     CDS             <1..595
                     /gene="gpmA"
                     /locus_tag="PLT35_13955"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOG30376.1"
                     /translation="DILYTSVLTRAIQTANLALDAADRAWIPVERSWRLNERHYGALQ
                     GLNKAETAAKHGMEQTSLWRRSYDVPPPALTPDDPRHPSRDPRYAGLAPADLPLTECL
                     KDTVNRFLPVWHETIAPAVKSGQRVLIAAHGNSLRALVKYLDNIPDADIVGLNIPTGI
                     PLVYELTDELTPTRSYYLGDPEAVKKAAEAVAAQLRK"
     gene            636..1859
                     /locus_tag="PLT35_13960"
     CDS             636..1859
                     /locus_tag="PLT35_13960"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOG30377.1"
                     /translation="MRSTIVAAAVVSAVLAAGPSPSATRLEGRARGRGAAVPRTSAPQ
                     APAPLRGFTIPLVDLAAQPFRQVVVDREPGQYLGHPTTVLLEDGRTILAVYPKGHGRG
                     AIVLKRSGDGGRTWSPRLPTPKSWETSLETPTIHRVVDARGVKRLIVFSGLYPIRMAV
                     SSDDGATWSELAPIGAFGGIVAMASVVKLETGPGHYMALFHDDGRFFAAKAAEGPRKF
                     VVYKTVSVDGGLTWGAPEAIASHPAAHLCEPGAIRSPDGRQIAVLLRENSRAFNSFLI
                     VSNDEGRTWSEPREAAGALTGDRHVFRYAPDSRLVATFRDTALETPTRGDWVAWVGTY
                     DDVVNGREGQYRVRLMDNLDEWDCAYPGLELLPDGTFVATTYGHWEPAAPPYVMSVRF
                     TLAEIDALAKEAARR"
BASE COUNT          291 a          694 c          671 g          255 t
ORIGIN      
        1 cgacatcctc tacacgagcg ttctcacccg cgcgatccag accgcgaacc tcgctctcga
       61 cgccgctgac cgcgcgtgga tcccggtcga gcgcagttgg cgcctcaacg agcgccacta
      121 cggcgcgctg caggggctga acaaggccga gacggccgcc aagcacggca tggagcagac
      181 gagtctctgg cgccggagct acgacgtgcc tccacccgcg ctgacgcccg acgatccgcg
      241 ccatccgagc cgcgacccgc gctatgcggg cctggcgccc gcggacctgc cgctgaccga
      301 gtgcctcaag gacaccgtca accggttcct gccggtgtgg cacgaaacca tcgccccggc
      361 cgtgaagtcc gggcagcggg tgctcatcgc cgcgcacggc aacagcctgc gggcgctcgt
      421 gaagtacctg gacaacatcc ccgacgcgga catcgtcggc ttgaacatcc cgacgggcat
      481 accgctcgtg tacgagctga ccgacgagct gacgccgacc aggagctact atctcggcga
      541 tccggaggcc gtgaagaagg ccgcggaagc cgtcgcggcg cagctcagga agtaggcggg
      601 gccccggcgc gccagccggg gccgggaggc cacgcatgag aagcacgatc gtcgcggcag
      661 ccgtcgtgtc tgcggtgctt gcggccgggc cctctccctc ggcgacgcgg ctggagggcc
      721 gcgcccgagg ccggggggcc gcggttcccc ggacgtccgc tccgcaggcg cccgctccgc
      781 tgcgcggctt cacgatcccg ctcgtcgacc tggcggcgca gccgttccgc caggtcgtcg
      841 tcgatcgcga gcccggccag tatctcgggc atccgacgac cgtgctcctc gaagacggcc
      901 gcacgatcct ggccgtctat ccgaagggcc acgggcgcgg cgcgatcgtg ctgaagcgca
      961 gcggcgacgg cggccggacg tggtcgccgc gcctgcccac gccgaagagc tgggagacgt
     1021 cgctcgaaac gcccaccatc caccgcgtcg tcgacgccag gggcgtgaag cgcctgatcg
     1081 tgttctcggg cctctatccc atccgcatgg cggtgtcgtc cgacgatggg gcgacgtgga
     1141 gcgagctggc gcccatcggg gcgttcggcg gcatcgtggc catggcctcc gtcgtgaagc
     1201 ttgaaaccgg gcctgggcac tacatggccc tcttccacga cgacgggcgc ttcttcgccg
     1261 cgaaggcggc ggagggcccc aggaagttcg ttgtctacaa gaccgtgtcc gtcgacggcg
     1321 gcctgacatg gggcgcgccg gaagccattg cgtcgcaccc cgccgctcac ctctgcgaac
     1381 cgggcgcaat ccggtcgccc gacggccggc agatcgcggt gctgctgcgc gagaacagcc
     1441 gggcgttcaa ctcgttcctg atcgtgtcga acgacgaggg gcggacctgg tccgagccgc
     1501 gcgaggcggc cggcgcgctg acgggcgacc gccacgtgtt ccgctacgcg ccggacagcc
     1561 ggctggtcgc cacgttccgg gacaccgcgc tcgagacgcc gaccaggggc gactgggtgg
     1621 cgtgggtcgg cacgtacgac gacgtcgtga acggccgcga ggggcagtac cgcgtccggc
     1681 tcatggacaa cctcgacgag tgggactgcg cctatcccgg cctggagctg ctgcccgacg
     1741 gcacgttcgt cgcgacgacg tacggccact gggagcccgc cgcaccgccc tacgtcatga
     1801 gcgtccgctt cacgctggcg gagatcgacg cgctggcgaa ggaggccgcc cggcggtagg
     1861 cgcccgatga ccctgcccga ttgcaagacg aagatcgtcg ccaccatcgg c
//
LOCUS       DAOSIF010000572         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_52157_length_1908_cov_3.525634, whole genome shotgun sequence.
ACCESSION   DAOSIF010000572 DAOSIF010000000
VERSION     DAOSIF010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52157_length_1908_cov_3.525634"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1362)
                     /locus_tag="PLT35_13965"
     CDS             complement(61..1362)
                     /locus_tag="PLT35_13965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG30378.1"
                     /translation="MSNPMNHPAAYRRRRFANWFPLGITYATYYMGRYNLNVSSTTIM
                     GQFHLSKADFGLIATAGFWTYALSVALNGPLADRIGGKKSILIGAAGASLVNLVIGLL
                     FLNGWQTKIIVGMSLLYAVNQYFQSFGALSVVKVNAPWFHVRERGVFGGVFGIMISSG
                     YFLALTVGGWILAFFPWYWVFLTPSLLLAVMFVVDALLVKDRPSQAGHPDFDTGDATS
                     KDADKDTPVDVGYLVKRVFSNPVILTIIAAEFCTGFVRQGVMLWFVPFLKEVHGIEHG
                     SAWFTAASTGVTVGGILGGLLCGFLSDRVFGSRRPPVAFLFYLGQIVALVWLGRTASP
                     AMAALLIPFTCMWIFGVHGMLSATSAMDFGGTKAAATVTGICDGVQYLASGLTGFVLG
                     GALDRFGWTAWTWMIIPFSAAGALLMTRLWHETPLRARGAH"
     gene            complement(1366..>1908)
                     /locus_tag="PLT35_13970"
     CDS             complement(1366..>1908)
                     /locus_tag="PLT35_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30379.1"
                     /translation="EARGEARLVSARSPEGYAITAPGFRGSLVNRFNAEIRERYLSRP
                     SFYEEGTLLIEFARGLGDGFRASLERFDPDILARAAALYIAVSFEESFRRNAARYRPG
                     REASILFHKVPDRDMLGYFRHNDWDAITGGAPDGYLQLTGANVPFVSMPNEPESSDAA
                     VLRRRYAGALGRLLHLYRTR"
BASE COUNT          335 a          637 c          684 g          252 t
ORIGIN      
        1 agctcgacgg tcggcaccgc gggaccggct ccggcgcgcg ccgggcgttc ggccggccgc
       61 tcagtgcgcg ccacgggccc tcagcggcgt ctcgtgccag agccgcgtca tgaggagcgc
      121 ccccgccgcg gagaagggga tgatcatcca ggtccacgcc gtccagccga agcgatcgag
      181 cgcgccgccc aggacgaagc cggtcaggcc ggaagcgagg tactgcacgc cgtcgcaaat
      241 gccggtcacg gtggcggcgg ccttcgtgcc tccgaagtcc atggccgacg tcgccgacag
      301 catgccgtga acgccgaaga tccacatgca ggtgaaggga atcagcagcg ccgccatggc
      361 gggggaggcc gttcgcccca gccagacgag ggccacgatc tggccgaggt agaagaggaa
      421 cgccaccggc ggccggcgcg atccgaacac gcggtcggac aggaacccgc acagcaggcc
      481 tccgaggatg ccgccgacgg tgacgccggt cgaggccgcc gtgaaccacg ccgacccgtg
      541 ctcgatccca tgcacttcct tcaggaacgg cacgaaccag agcatcacgc cctggcgcac
      601 gaacccggtg cagaactcgg ccgcgatgat cgtcaggatg acggggttcg agaacacgcg
      661 cttcacgagg tagccgacgt ccaccggcgt gtccttgtcg gcgtccttcg aggtcgcgtc
      721 gcccgtgtcg aagtccgggt gtcccgcctg cgagggcctg tccttcacga gcagcgcgtc
      781 cacgacgaac atcaccgcga ggagcagcga cggcgtcagg aacacccagt accaggggaa
      841 gaatgccagg atccagccgc cgacggtcag ggccaggaag taccccgacg agatcatgat
      901 gccgaagacg ccgccgaaga cgccgcgctc cctgacgtgg aaccacggcg cgttgacctt
      961 gaccaccgac agcgcgccga acgactggaa gtactggttg acggcgtaga gcagcgacat
     1021 gccgacgatg atcttcgtct gccacccgtt caggaacagc agcccgatga cgaggttgac
     1081 gagcgaggcg ccggccgccc cgatgaggat cgacttcttg ccgccgatgc ggtcggcaag
     1141 cggcccgttg agggccaccg acagggcgta ggtccagaag cccgccgtcg cgatcaggcc
     1201 gaagtccgcc ttcgacaggt ggaactggcc catgattgtc gtggacgaga cgttcaggtt
     1261 gtaccgcccc atgtagtacg tcgcgtaggt gatgccgagg gggaaccagt tggcgaagcg
     1321 ccggcggcgg taggccgccg ggtggttcat cgggttgctc atgggctacc tcgtccggta
     1381 gaggtgcagc agccgcccga gggcgccggc gtaccgccgg cggagcacgg cggcgtccga
     1441 cgactcgggc tcgttgggca tggacacgaa gggcacgttc gcgccggtca gctgcaggta
     1501 gccgtccggc gcgccgcccg tgatcgcgtc ccagtcgttg tgccggaagt accccagcat
     1561 gtcgcggtcg ggcaccttgt ggaacaggat cgatgcctcg cggcctggcc ggtagcgggc
     1621 ggcgttgcgg cggaacgatt cctcgaagga gaccgcgatg tacagggcgg cggcgcgcgc
     1681 caggatgtcg gggtcgaacc gctcgaggct cgcgcggaag ccgtcgccga ggccccgcgc
     1741 gaactcgatg agcagcgtgc cttcctcgta gaacgacggc ctcgagaggt accgctcgcg
     1801 gatctcggcg ttgaaccggt tgacgagcga gccgcggaac cccggggcgg tgatggcgta
     1861 cccttcgggc gaccgtgccg agacgaggcg ggcctctccc cgggcctc
//
LOCUS       DAOSIF010000573         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_52445_length_1901_cov_2.796858, whole genome shotgun sequence.
ACCESSION   DAOSIF010000573 DAOSIF010000000
VERSION     DAOSIF010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52445_length_1901_cov_2.796858"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLT35_13975"
     CDS             complement(<1..483)
                     /locus_tag="PLT35_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOG30380.1"
                     /translation="MSRVDGPGARIGLTHGAGGRAMRALIEQVCLAGLGAGALDDGIG
                     LQALDDGAAVRAGSGWMILTTDSHVVKPIFFPGGDIGRLAVCGTVNDLAVMGATERPI
                     LTCAVIVEEGFPRDDLERVYLSIRTACEEAGAAVAAGDTKVMGRGELDGLVLNTAGVA
                     F"
     gene            complement(480..1625)
                     /gene="hypD"
                     /locus_tag="PLT35_13980"
     CDS             complement(480..1625)
                     /gene="hypD"
                     /locus_tag="PLT35_13980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOG30381.1"
                     /translation="MATPDREGPFREAAFRDPARASAMSGAIAKAVSALGRSPVTVMH
                     VCGSHEQAIAKYGLRAVLPRELHVIMGPGCPVCVTDAPEVDEGVALALQGVHVATYGD
                     MLRVPGSSLSLADAQAEGARVHLVYSVDRAVDLARRTRGEVVFFATGFETTAVATAAA
                     VSRGVPSNFSILCAHKYIPPAMDVVAGIPGSKVEGFLAAGHAATITGWSVFEPFVARH
                     RLPVVVAGFEPLDILAALLRAVERIRAGSPRVDNVYPRCVTKDGNRRAQEAMWRVFER
                     ADGRWRGIGEVPGGSLRLREAFGGLDARRRFAIDATGLPTRTPRPDAAQCLCGAIMSG
                     IASPGDCPLYADACTPEHPVGACMVSSEGTCRIWHEYGGHPDLGDIP"
     gene            complement(1612..>1901)
                     /locus_tag="PLT35_13985"
     CDS             complement(1612..>1901)
                     /locus_tag="PLT35_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOG30382.1"
                     /translation="GVPGRVVAIEGHTATVDFWGVRRQVLLDIVDEPVSVGDYVLNHV
                     GYAIRRIPDDEIAETLALFEAVLASQESGDLLADGVRSEIAGTGAGGDGDA"
BASE COUNT          283 a          719 c          649 g          250 t
ORIGIN      
        1 gaacgccaca ccggccgtgt tgagcacgag gccgtcgagt tccccgcgcc ccatcacctt
       61 cgtgtcgcct gccgccaccg ccgcgccggc ttcctcgcac gccgtccgga tcgacaggta
      121 cacccgctcg agatcgtccc gcggaaaacc ctcctcgacg atgacggcgc aggtcaggat
      181 cgggcgctcg gtcgcgccca tgacggcgag atcgttgacc gtgccgcaga cggccaggcg
      241 gccgatgtcg ccgccgggaa agaagatcgg cttcacgacg tgcgagtccg tcgtcaggat
      301 catccacccg ctgccggccc ggacggccgc gccgtcgtcc agcgcctgaa ggccgatgcc
      361 gtcgtcgagc gccccggcgc ccaggccggc caggcagacc tgctcgatga gcgcgcgcat
      421 ggcgcgcccc ccggcgccgt gcgtcaggcc gatcctcgcg cctggcccgt cgacgcgact
      481 cacgggatgt ctccgagatc gggatggccc ccgtactcgt gccagatccg gcaggtgccc
      541 tcgctgctga ccatgcacgc gccgacggga tgctcgggcg tgcacgcgtc tgcatagagc
      601 gggcagtcgc cgggcgaggc gatgcccgac atgatggcgc cgcacaggca ctgcgccgca
      661 tccgggcggg gcgtccgcgt cgggagtcct gtggcgtcga tagcgaatcg gcgccgcgcg
      721 tcgaggccgc cgaaggcctc tcggaggcgg aggctgccgc ccggtacctc gccaatcccc
      781 ctccagcgcc catccgcgcg ctcgaacacg cgccacatcg cctcctgcgc ccgccggttg
      841 ccgtctttcg tgacgcagcg cgggtagacg ttatcgacgc ggggcgaacc ggcgcgaata
      901 cgctccacgg ctctcaagag ggcggcgagg atgtccagcg gctcgaagcc ggcgacgacg
      961 accggcaggc gatggcgcgc cacgaacggc tcgaacacgc tccagccggt gatcgtggcg
     1021 gcatggcccg cggccaggaa cccctcgacc ttcgagccgg ggatgcccgc caccacgtcc
     1081 atcgcgggcg ggatgtactt gtgtgcgcac aggatcgaga agttggacgg cacgcccctc
     1141 gacacggcag cggctgtcgc gacggccgtt gtttcgaagc ccgtcgcgaa gaaaacgacc
     1201 tcgccgcggg tccggcgggc cagatcgacg gcccggtcca cgctgtagac gaggtggacg
     1261 cgcgccccct cggcctgggc atccgccagc gacagggacg agcccggcac gcgcagcatg
     1321 tcgccgtagg tggcgacgtg cacgccttga agcgccaggg cgacgccttc gtccacttcc
     1381 ggggcgtccg tgacgcagac cgggcagccg ggccccatga tgacgtgcag ttcgcgcggc
     1441 agcaccgctc gaagcccgta cttggcgatg gcctgctcgt ggctcccgca cacgtgcatc
     1501 acggtcacgg ggctccgccc gagggccgac accgccttcg cgatggcgcc gctcatggcc
     1561 gaggcgcggg ccggatccct gaacgccgcc tcccggaacg gcccttcgcg atcaggcgtc
     1621 gccatcgccc ccggccccgg tgccggcaat ctccgatcga acgccgtccg ccaggaggtc
     1681 cccggactcc tgactcgcca gcacggcctc gaacagcgcc agcgtctccg caatctcgtc
     1741 gtcgggaatg cggcggatcg cgtagccgac gtggttgagc acgtagtcgc ccaccgacac
     1801 cggttcgtcc acgatgtcga gcaggacctg gcgccggacg ccccagaagt ccaccgtcgc
     1861 ggtgtggccc tcgatggcca cgacgcggcc cggcacgcct a
//
LOCUS       DAOSIF010000574         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_53393_length_1876_cov_4.728171, whole genome shotgun sequence.
ACCESSION   DAOSIF010000574 DAOSIF010000000
VERSION     DAOSIF010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53393_length_1876_cov_4.728171"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLT35_13990"
     CDS             complement(<1..714)
                     /locus_tag="PLT35_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOG30383.1"
                     /translation="MRIGAYVSMGNILERLPVGERIGIAFSGGLDTCAALHWMRRRGA
                     VPYAYTAHLGQPDELDYEAIPRKALDYGAERARLIECRGQLVAEGLAALQCGAFHIST
                     AGQFYFNTTPIGRAVTGTMLVSAMQEDGVLIWGDGSTFKGNDIERFYRYGLLVNPKLR
                     IYKPWLDQQFIDDLGGRAEMSAYLDGAGLEYRMSADKAYSTDSNILGATHEAKDLEFL
                     DRGVSIVRPIMGVASWRDDV"
     gene            830..>1876
                     /locus_tag="PLT35_13995"
     CDS             830..>1876
                     /locus_tag="PLT35_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30384.1"
                     /translation="MTAARTGFRRVGRRAAALPLAILAVAGAARAREPAQDGRQPLVL
                     RPAPSPLAAEGATGPSVALDGLHFETAAGRVALPAAMSRPIAAGASASREFLLADDRV
                     VRLVASPGLRAYTIRLSAEPSEDILGWGLSIAAAPGERFTGLLERVADGPQEASRAPD
                     LAAGLDLCGQRVDTLVKPATSAYAPFYVSSGGYGVFVKGAWPGRFDVCAADPGRVAIE
                     FEGPSLELKVYLDGDPAAIVKAHAMEAGPPVLPPKWMLRPWRWRDEHAHRPAYADGTP
                     VTGPFNSQVMEDVLMMRAFGIPNGVYCIDRPHGPDRLGGGGDGEIDPARLPRFADMVG
                     WLNGQGMQALLRIAP"
BASE COUNT          229 a          657 c          721 g          269 t
ORIGIN      
        1 cacgtcgtcg cgccaggatg cgacgcccat gatcgggcgg acgatggaga cgccgcggtc
       61 gaggaactcg aggtccttgg cctcgtgggt ggcgccgagg atgttcgagt cggtggagta
      121 ggccttgtcg gcgctcatcc tgtactcgag gccagcgccg tcgaggtagg cggacatctc
      181 ggcccggccg ccgaggtcgt cgatgaactg ctggtcgagc cagggcttgt agatgcgcag
      241 cttcgggttc acgaggaggc cgtagcggta gaagcgctcg atgtcgttgc ccttgaacgt
      301 gctgccgtcg ccccagatga ggacgccgtc ctcctgcatg gccgacacga gcatcgtgcc
      361 cgtgaccgcg cggccgatgg gcgtggtgtt gaagtagaac tggccggcgg tcgagatgtg
      421 gaacgcgccg cactggaggg cggcgaggcc ctcggcgacg agctggccgc ggcactcgat
      481 gaggcgcgcg cgctcggcgc cgtagtccag cgccttgcgc gggatggcct cgtagtcgag
      541 ctcgtccggc tggccgaggt gggcggtgta ggcgtagggg accgcgccgc gccggcgcat
      601 ccagtgcagg gccgcgcagg tgtcgaggcc gcccgagaag gcgatgccga tccgctcgcc
      661 gaccggcagg cgttcgagga tgttgcccat cgatacgtat gctccaatcc gcataagtat
      721 acatccatcg gggatgcgcg cccggcgcgc cccggggccg gggcgaacgc cgccggccga
      781 atagggtaga ctgcggccgc acactggcgg gtacccccgg gggcggcgca tgacggcagc
      841 gcggacgggc tttcgcaggg tcggccggcg cgcggcggcc ctgccgctgg cgatcctggc
      901 ggtggcgggc gcggcccgcg cccgggaacc cgcgcaggac gggcggcagc cgctcgtgct
      961 ccgccccgcc ccctcgccgc tcgccgccga gggcgcgacc ggcccgtcgg tcgcgctcga
     1021 cgggctccat ttcgagacgg ctgccgggcg cgtcgcgctg ccggcggcca tgtcccgccc
     1081 gatcgcggcc ggcgcgtcgg cctcgcgcga gttcctgctg gccgacgacc gcgtcgtgcg
     1141 cctcgtcgcc tcacccggcc tccgcgccta caccatccgc ctgtccgccg agccgtcgga
     1201 agacatcctg ggctggggcc tctcgattgc cgccgcgcct ggcgagcgct tcacgggcct
     1261 cctggagcgc gtggcggacg ggccgcagga ggcctcccgg gcgccggacc tcgccgcggg
     1321 cctggacctg tgcggccagc gggtggacac gctcgtgaag ccggcgacgt cggcgtacgc
     1381 gccgttctac gtgtcgtccg gcggctacgg cgtgttcgtg aagggcgcgt ggcccggccg
     1441 cttcgatgtc tgcgcggccg atcccggccg cgtcgccatc gagttcgagg ggccgtcgct
     1501 ggaactgaag gtctacctgg acggcgatcc cgcggccatc gtgaaggccc acgccatgga
     1561 ggcgggcccg cccgtgctgc cgccgaagtg gatgctccgc ccgtggcggt ggcgcgacga
     1621 gcacgcgcac cgcccggcgt acgccgacgg cacgcccgtg accggcccgt ttaactcgca
     1681 ggtcatggag gacgtgctga tgatgcgggc gttcggcatc ccgaacggcg tctactgcat
     1741 cgatcgtccg cacggcccgg atcggctcgg cggcggcggc gacggcgaga tcgatccggc
     1801 ccgcctgccg cgcttcgcgg acatggtggg ctggctcaac ggccagggca tgcaggcgct
     1861 gctgcggatc gcgccg
//
LOCUS       DAOSIF010000575         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_53531_length_1873_cov_3.672717, whole genome shotgun sequence.
ACCESSION   DAOSIF010000575 DAOSIF010000000
VERSION     DAOSIF010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53531_length_1873_cov_3.672717"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="PLT35_14000"
     CDS             complement(<1..1341)
                     /locus_tag="PLT35_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30385.1"
                     /translation="MFACLYLPPSPMRRAVPAIGPARDRTSLAEIARKVSPRVDVRRE
                     NLVVLDVEGLERLVGDARAVGERLRREAADRGFAPVHVALAGTRTAAMLLAAARAGVT
                     VVAPGGEADALAPLPLRTLGELASSAQPLAPASRPPVHSLESRTTNPESRIPGPGCLS
                     TLSRWGLRTLGELAALPSVELSERLGQDGVRWQRLARGEDTEPLVPAREDEPFESSLD
                     LEWPIADLEPLSFVLGRLLDPLCARLESRDRGAAALHLRLRLVTRALHLRSLQLPAPM
                     RDPKVLRTLLLLDLEAHPAPAGIDAVAIVIDPTPGRIVQESLLTRALPAPELVSTLVA
                     RLQALMGEGRCGSPATVDSYRPGAFAMTPFAVEQKQESRSRHAEAGMQEPSLEPLAPN
                     PESRIPSAALRRFRIPIPARVLIDGHRPTSVRTDRQGLSGGQVRGWAGPWRTSGG"
     gene            complement(1356..>1873)
                     /locus_tag="PLT35_14005"
     CDS             complement(1356..>1873)
                     /locus_tag="PLT35_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30386.1"
                     /translation="GGLAALVDTFDMADVASMAAAGVRLDRLLWVRGESSTSAFPPLT
                     NGRRQPGADRALARALKAANLILQSGAMSLVALDVADAPLQALRRVPITTWLRLQKVV
                     EGQSTAALIVGSVPMSRSAGGVTLGLRQLGARGLAPGAGGIESEARIQRARLVPGMGA
                     PVRLTAAALVA"
BASE COUNT          282 a          693 c          676 g          222 t
ORIGIN      
        1 cccgcccgac gtgcgccacg gcccggccca accgcgcacc tgcccgccgg agagcccctg
       61 ccgatcggtc cgcacgctgg tcgggcgatg gccgtcgatg agcacacgcg cgggaatggg
      121 aatgcggaaa cggcgcaggg ccgcgctcgg gattcgggat tcgggattcg gggccagggg
      181 ctcgaggctc ggctcctgca tgccggcttc tgcatgccgg cttctggatt cctgcttctg
      241 ctccaccgcg aagggcgtca tcgcgaacgc cccgggcctg tacgaatcca ccgtcgccgg
      301 cgacccgcac cgcccctcgc ccatgagcgc ctgcaggcgc gcgacgagcg tcgagacgag
      361 ctcgggcgcc ggcagcgcgc gcgtcagcag cgactcctgg acgatgcggc ccggcgtggg
      421 gtcgatgacg atggcgaccg cgtcgatgcc cgccggcgcc ggatgcgctt cgagatccag
      481 cagcagcagc gtgcgcagca ctttcggatc gcgcatgggc gcgggcagct gcagactgcg
      541 caggtgcaag gcccgcgtga cgaggcgcag gcgcaggtgc agggccgcgg cgccgcgatc
      601 gcgggactcg aggcgcgcgc acaggggatc gagcagccgc ccgaggacga acgacagcgg
      661 ctcgagatcg gcaatgggcc attccagatc gagcgaggac tcgaagggct cgtcttccct
      721 ggcgggaacc agcggctccg tgtcttcgcc gcgcgccagg cgctgccagc ggacgccgtc
      781 ctgccccagc cgctccgaca gttcgacgga cggcaacgcg gccagttcgc ccagcgtgcg
      841 caggccccag cgggacaggg tggagagaca gccgggaccc gggattcggg attcgggatt
      901 cgtggttcgg gattcgaggc tgtggactgg gggccgggaa gcgggggcca ggggctgggc
      961 gctagacgcg agttcgccga gtgtccgaag aggcagcggc gcgagcgcgt ccgcctcccc
     1021 tcccggcgcc accaccgtca ctcccgctcg cgcggcggcg agcagcatgg ctgccgtccg
     1081 ggtgcccgca agcgccacgt gcaccggcgc aaagccgcga tccgccgctt cccggcgcag
     1141 gcgttcgccg acggcccgcg cgtcgccgac caggcgctcg aggccctcga cgtcgagcac
     1201 caccaggttc tcgcgccgca cgtccacgcg cggcgacacc ttcctggcaa tctcggcgag
     1261 gctcgtgcga tcgcgagccg ggccgatcgc cggcacggcc cggcgcatgg gtgaaggcgg
     1321 caggtagaga cacgcgaaca tggcgcgcct gcccgtcagg ccaccagcgc cgccgccgtc
     1381 aggcgcaccg gcgcgcccat gcccggcacc agtctcgcgc gctggatcct cgcctcgctc
     1441 tcaatcccgc cagcgcccgg cgccaagccc ctcgcgccca gctggcgcag ccccagcgtc
     1501 acgcccccag cgctccggct catgggtacg gatccgacga tgagcgcggc cgtggactgt
     1561 ccttcgacga ccttctgcaa ccgaagccag gtcgtgatgg gaacccgccg cagggcctgg
     1621 agcggcgcat cggccacgtc gagggccaca agcgacatgg cacccgactg caggatgaga
     1681 ttcgcggctt tcagcgcgcg cgcgagggcg cgatcggcgc cgggctggcg gcggccattc
     1741 gtcaggggcg ggaatgccga ggtcgaggat tcgccgcgca cccacagcag acgatccagc
     1801 cgcacgccgg cagccgccat cgacgccacg tcggccatat cgaaggtgtc gacaagagcc
     1861 gccaatccac ccc
//
LOCUS       DAOSIF010000576         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_54217_length_1856_cov_4.545808, whole genome shotgun sequence.
ACCESSION   DAOSIF010000576 DAOSIF010000000
VERSION     DAOSIF010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54217_length_1856_cov_4.545808"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PLT35_14010"
     CDS             complement(<1..1099)
                     /locus_tag="PLT35_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30387.1"
                     /translation="MRVFPGAIAALTVSLAAPALTAQQPPAAAPAAARPAAPPRLTLD
                     VDSIMRGPDLVGYPPSGLRWSGDSRDLFFEWRKPGEAEASTYVLSQHDAVPRRLTEEE
                     RKLAPPAAGGAWDEARRRVVFAERGDIVVIDTVARTRQQITRTTAVESSPRWARGGAA
                     VTFVSQNNLFIVPLASGTLRQLTDVKPRRRDARDSDSQRALKEQEAALIQFTKEAAAR
                     RKADEEARKPDALPEFELTERQAVQDLLLDPSETRVYLVVTERADAKASIVPNYVTES
                     GYTEDIASRPKVGDRQAARRLAVLDLRSRAVVWADAGFAGTIKAGGRDQPREVDWSAP
                     LFSPDGSLVIAQVRSADFKDRFIKEARSAAAL"
     gene            1287..>1856
                     /locus_tag="PLT35_14015"
     CDS             1287..>1856
                     /locus_tag="PLT35_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30388.1"
                     /translation="MCDCRASVIRAGAIAALVAAMASPSYAQAPQPAPAAPAALAAPA
                     RQLPLEEAIRLALQNNLNLRVERMNPELQDLAIDQARTAWTPNLIGSATATRRLSPIS
                     SFFAGATDVLRNNGFTSEVGVNQVLPWGANYTVTWDTSRTRTNSVFNSPNPSLGSNLN
                     FSFSQPLLKDFKVNAARHQLLVSKANREIS"
BASE COUNT          267 a          669 c          634 g          286 t
ORIGIN      
        1 tgagcgcggc ggcgctgcgg gcttccttga taaagcggtc cttgaagtcg gccgaccgca
       61 cctgcgcgat gacgaggctg ccgtccggcg agaacagggg cgcggaccag tccacttccc
      121 tcggctggtc cctgcctccg gccttgatcg tgccggcgaa acccgcatcg gcccagacaa
      181 cggcgcggct tcgaaggtcg agcacggcca ggcggcgcgc ggcctggcgg tcgccgacct
      241 tcggccgcga cgcgatgtct tccgtgtagc cggactcggt cacgtagttc ggcacgatgc
      301 tcgctttcgc gtccgcccgc tccgtgacga ccagatagac gcgcgtctcc gagggatcga
      361 gcagcaggtc ctgcacggcc tgccgctcgg tgagctcgaa ctcgggcagc gcgtccggct
      421 tgcgcgcctc ctcgtcggcc tttcgccgcg ccgccgcctc cttcgtgaac tggatcaggg
      481 cggcttcctg ttccttcagg gcgcgctggc tgtccgagtc gcgcgcatcg cgccgccgcg
      541 gcttcacgtc ggtgagctgc cggagcgtcc cggaggccag cggcacgatg aacaggttgt
      601 tctgcgagac gaacgtgacg gccgcgcctc cccgggccca ccgcgggctg gattccaccg
      661 ccgtcgtgcg cgtgatctgc tggcgcgtgc gcgccaccgt gtcgatgacg acgatgtccc
      721 cccgctcggc gaagacgacg cggcggcggg cctcatccca cgccccgccg gccgccgggg
      781 gggcgagctt ccgctcctcc tccgtcagcc gtctcggaac ggcgtcgtgc tgcgacagga
      841 cgtaggtcga cgcctcggcc tcgcccggct tgcgccactc gaagaagagg tcccgggagt
      901 cgccggacca gcggaggccc gacggggggt agccgaccag atccgggccg cgcatgatgc
      961 tgtccacgtc gagcgtcagc ctcggcggag cggccgggcg ggcggcggcg ggagcggcgg
     1021 cgggcggctg ctgggccgtc agcgccgggg ctgcgaggga cacggtgagg gcggcaatcg
     1081 ctcctgggaa cacgcgcatg ggtggcctgc cttcgctcgc ggattgcgaa acgcacgaga
     1141 tcggatcgaa aacggctggt ctggcgcggt gctagtatac ccggtgagga gtccctgaac
     1201 cggcttcggt cccgtctcgt ctatttcctc taaggactgg cgtgtcccgc cgccctcgtg
     1261 atccttcagg agtggagcag tccgtcatgt gtgattgccg tgcctcagtc atccgggctg
     1321 gcgcgatcgc agcgctggtc gccgccatgg cctcgccgtc gtacgctcag gccccgcagc
     1381 ccgcgcccgc ggcgccggcg gcacttgccg ccccggcccg tcagctgccg ctagaagaag
     1441 ccattcgcct cgccctccag aacaacctga atctcagggt cgagcggatg aatcccgagc
     1501 tccaggacct cgccatcgac caggccagga ccgcttggac gccgaacctg atcgggtcgg
     1561 ccacggccac ccgccggctg agccccatca gcagcttctt cgcaggcgcg acggacgtgc
     1621 tccggaacaa cgggttcacc tcggaagtcg gcgtgaacca ggtgctgccg tggggcgcca
     1681 actacacggt cacctgggac acatcgcgca cccggaccaa cagcgtgttc aacagcccga
     1741 acccctcgct cgggtcgaac ctgaacttca gcttcagcca gccgctcctg aaggacttca
     1801 aggtgaacgc ggcgcgccac cagctcctcg tgagcaaggc caaccgcgag atctcg
//
LOCUS       DAOSIF010000577         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_54219_length_1856_cov_3.584675, whole genome shotgun sequence.
ACCESSION   DAOSIF010000577 DAOSIF010000000
VERSION     DAOSIF010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54219_length_1856_cov_3.584675"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLT35_14020"
     CDS             <1..497
                     /locus_tag="PLT35_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30389.1"
                     /translation="DAAVAEADVVRLALNQSAVVTPEAVPGERFAGRVVEIGASALPV
                     AGSGAAAREFRVKVRLETADRRLRPGLTCDAEILTAERQHALVVPLQAVVIRPGRDGR
                     DEAGLFVAAGRSVRFQPVTAGIIGGLDIEVSGVEDGAVVVTGPYQALRDLQDGQAVRV
                     RSRQ"
     gene            494..>1856
                     /locus_tag="PLT35_14025"
     CDS             494..>1856
                     /locus_tag="PLT35_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG30390.1"
                     /translation="MTRLPGHVVETACPLDCPDSCSLSVSLAGGRLSAIDAGGANPVT
                     GGFICAKVRRFADRVYGPDRLLQPGFRLGPPGSGTFRWVSWDEALDRVVSAMREARDG
                     WGAASVLPYCYGGSNGLLSQDSADARLFRRFGASRLARTLCAAVTGAAYESMYGKMPS
                     VTYQDYRHAALIVVWGANPSASGPHLVPYIREARSAGAKLVVVDPRATPLARGADLHL
                     AVRPGTDVAVALALHRHLFEHALADQRFLDAHATGAERLRARALPWTVDRAAEVAGVR
                     ADDLARFADLYAQSAPALIRCGWGLERNRNGGSAAAAILALPAVAGKFGVRGGGFTLS
                     NSAAWGLAKPWIGAEEPPTRVINMAHLGRVLTEPLDPPVKVLFVYNCNPLATAPNQNL
                     VRRGLARADLTTIVFDQVLTDTARHADIVLPAPTFLEQYDLARSYGAIGLQLVRPVID
                     ACGS"
BASE COUNT          217 a          689 c          685 g          265 t
ORIGIN      
        1 tggacgcggc ggtggccgag gccgacgtgg ttcgcctcgc cctgaaccag tccgccgtgg
       61 tgacgccgga ggccgtgccg ggggagcgtt tcgccgggcg cgtcgtcgag attggcgcca
      121 gcgcgctgcc ggtcgccggc agcggagccg cggcccgcga gttccgcgtg aaggtgcgcc
      181 tcgagacggc cgatcgccgc ctgcggccgg gcctcacctg cgacgccgag attctgaccg
      241 ccgaacgcca gcacgccctc gtcgtgccgc tgcaggccgt cgtgatacgg cccggccgcg
      301 acgggcgcga cgaggccggc ctgttcgtcg ccgccggccg cagcgtgcgc ttccagcccg
      361 tgacggctgg catcatcggc ggcctcgaca tcgaggtgtc gggcgtcgaa gacggcgcgg
      421 tggtggtgac ggggccctat caggcgctgc gcgacctgca ggacggccag gccgtgcgcg
      481 tgcgatcccg ccaatgacca ggctgccggg acacgtcgtc gagaccgcct gcccgctgga
      541 ctgcccggat tcgtgcagcc tcagcgtgtc gctcgccggc ggccgcctgt ccgctatcga
      601 cgccggtggc gccaaccccg tgaccggcgg gttcatctgc gccaaggtca ggcggttcgc
      661 cgaccgcgtg tacggcccgg accggctgct tcagccgggg ttccgcctgg gtccgcccgg
      721 gtcgggcacg ttccggtggg tgagctggga cgaggcgctc gaccgcgtgg tgtcggccat
      781 gcgcgaggcc cgcgacggct ggggcgcggc ctcggtgctc ccctactgct acggaggctc
      841 gaacggcctg ctctcgcagg acagcgcgga tgcccggctg ttccgccggt tcggggcgtc
      901 gcgcctggcg cgcacgctct gcgccgcggt cactggcgcc gcctacgagt cgatgtacgg
      961 gaagatgccg tccgtcacgt accaggacta ccggcacgcg gcgctgattg tcgtctgggg
     1021 cgcgaacccc tcggcctcgg ggccgcatct ggtgccgtac atccgcgagg cccgcagcgc
     1081 cggggcgaaa ctggtggtcg tcgacccgcg cgccacgccg cttgcgcgcg gcgctgatct
     1141 ccacctggcg gtgcggccgg gcaccgacgt ggccgtggcc ctcgcgctcc accgccatct
     1201 gttcgagcac gcgctggcgg accagcgttt cctggacgcg cacgcgacgg gagccgaacg
     1261 gttgcgggcg cgcgcgctgc cctggaccgt tgatcgcgcg gctgaggtcg ccggcgtgcg
     1321 ggccgacgac ctcgctcgct tcgcggacct gtacgcgcag tccgcgccgg ccctcatccg
     1381 gtgcggctgg ggcctcgagc gcaaccgcaa cggcggcagc gccgcggcgg ccatcctggc
     1441 cctgccggcc gtggccggca agttcggcgt gaggggcggc ggcttcacgc tcagcaactc
     1501 ggcggcatgg ggtctcgcga agccctggat cggcgccgag gagcccccaa cccgggtgat
     1561 caacatggcc cacctgggcc gcgtcctcac ggagcccctg gatcctcccg tcaaagtgct
     1621 cttcgtctac aactgcaacc cgctggcgac ggcgccgaac cagaacctgg tgcgccgcgg
     1681 cctggcgcgc gccgatctga cgacgatcgt cttcgaccag gtgctgaccg acaccgcgcg
     1741 gcacgcggac atcgtcctgc cggcgccgac gttcctcgag cagtacgacc ttgcgcggag
     1801 ctacggcgcg atcggcctcc agctggtccg ccccgtcatc gacgcgtgcg gcagcg
//
LOCUS       DAOSIF010000578         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_54502_length_1849_cov_2.855072, whole genome shotgun sequence.
ACCESSION   DAOSIF010000578 DAOSIF010000000
VERSION     DAOSIF010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54502_length_1849_cov_2.855072"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PLT35_14030"
     CDS             complement(<1..1325)
                     /locus_tag="PLT35_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG30391.1"
                     /translation="MIALDLHVHTCLSPCGALDMHPGAIVREAVACGLDGVAICDHNA
                     ADNVAATGRAARAAGCLAVPGIEITTEEEVHVVALLPDEAAAGALHARVASALPGRNA
                     PGPFGEQVIANERAEVLGFNPSLLAGATSWSLERAVGEIHRAGGLAIAAHADRERFGI
                     VGQLGFVPPGLALDAVEVSAATGFGDGRARYADPLGLAAVTGSDAHRPGEIGRAVTFL
                     HLERPDAAEVGRAIKGVGGRSVLGGGRPMEDLAMHILDIAQNAVEAGATRVEVRVEED
                     EAADLLRIVVADNGRGMDQAAASRALDPFFTTRGTRAVGLGLPLLRHAAEAAGGSLRV
                     ASAPGAGTRVEAEFAWAHLDRAPMGDLETTVLVLLASHPDVDLDWTHRRGGGEYSVST
                     GDLRAALGGASLASPAGIALARAAVRQGEAGLRGPAGGASPSAATAATS"
     gene            complement(1328..1678)
                     /locus_tag="PLT35_14035"
     CDS             complement(1328..1678)
                     /locus_tag="PLT35_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30392.1"
                     /translation="MTITDLAALVEGTAIGTAEALAREAGGGYASDLLSDVMANARDG
                     DAWITLQRHVNTVAVAQLRNLAAIVIVNGRQPEADVLERAAAHDVAIVTTALTAFDAA
                     GRLYAAGLRGRGAS"
BASE COUNT          223 a          744 c          660 g          222 t
ORIGIN      
        1 caggacgttg ctgctgtggc ggccgatggt gatgcgccgc ccgccggccc gcgcagtccc
       61 gcctctccct ggcgcacggc ggcccgcgcc agcgcgatcc ccgccggcga cgcgagcgag
      121 gccccgccga gggccgcgcg cagatcgccg gtcgacacgc tgtactcacc cccgccgcgg
      181 cggtgcgtcc agtccagatc gacgtccggg tgcgaggcca gcagcaccag gacggtggtt
      241 tccaggtccc ccatcggcgc gcggtcgaga tgggcccagg cgaactccgc ctcgacgcgc
      301 gtgcccgccc ccggcgcgga ggcgacgcgc agcgaccccc cggccgcctc tgcggcgtgg
      361 cgcaggagcg ggagccccag cccgacggcg cgcgtcccgc gcgtcgtgaa gaacgggtcc
      421 agcgcgcggc tcgcggcggc ctggtccatg ccgcgcccgt tgtccgccac cacgatgcgg
      481 aggagatcgg ccgcctcgtc ttcctccacc cggacctcga cgcgcgtggc ccccgcctcg
      541 acggcgttct gcgcgatgtc gaggatgtgc atcgccaggt cttccatcgg ccgccctccg
      601 ccgagcacgc tccggccgcc cacgcccttg atcgcgcggc ccacctccgc cgcgtcgggc
      661 cgttcgagat gcagaaacgt gacggcccgt ccgatctcgc cggggcggtg ggcgtcggac
      721 ccggtgacgg ccgccaggcc cagcgggtcc gcgtagcggg cgcgcccgtc gccgaacccg
      781 gtggccgccg acacctccac ggcgtcgagg gcgaggccgg gcggcacgaa ccccagttgg
      841 cccacgatgc cgaagcgctc gcggtcggcg tgcgccgcga tggcaaggcc acccgcccgg
      901 tggatctcgc cgaccgcccg ctccagcgac cacgacgtcg cgccggcgag cagcgacgga
      961 ttgaagccga ggacctccgc gcgctcgttg gcgatgacct gctcgccgaa ggggcccggc
     1021 gcgttgcggc cgggcagcgc cgaggcgacg cgggcgtgca aagccccggc cgccgcttcg
     1081 tccggcagca gcgccacgac gtggacctcc tcttccgtcg tgatctcgat gcccggcacg
     1141 gcgaggcagc ccgccgcgcg cgctgcgcgc ccggtcgcgg cgacgttgtc cgcggcgttg
     1201 tggtcgcaga tcgccacgcc gtccaggccg caggcgacgg cctcgcggac gatcgccccc
     1261 gggtgcatgt cgagcgcacc gcagggcgag aggcacgtgt gcacgtgcag gtcgagcgcg
     1321 atcaccgtca ggacgcccct ctcccccgca ggccggcggc atagagccgg cccgccgcat
     1381 cgaacgccgt cagggcggtc gtcacgatgg ccacgtcgtg cgccgcggcc cgctcgagca
     1441 cgtccgcctc tggctggcgc ccgttcacga tgacgatggc ggccaggttc cgcagctgcg
     1501 ccaccgcgac ggtgttcacg tggcgctgca gcgtgatcca cgcgtcgccg tcccgggcgt
     1561 tggccatcac gtcggagagc aggtcagagg cgtagccgcc ccccgcttcc cgcgcgaggg
     1621 cctccgcggt gccgatggcg gtgccctcga cgagcgccgc cagatccgtg atcgtcatgc
     1681 ccgtccctcc gcgcgctccg acagctgcgc gatccgatcc aggagctgct gctgccgcac
     1741 gaacacgcag tcggcttcgt cggccgcccc gcgcaccacg tcctcggcga agctgaggca
     1801 ggtcggcgcg ccgcagggca ccctcgggca cctcgggccc ctccgccca
//
LOCUS       DAOSIF010000579         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_54601_length_1846_cov_2.683417, whole genome shotgun sequence.
ACCESSION   DAOSIF010000579 DAOSIF010000000
VERSION     DAOSIF010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54601_length_1846_cov_2.683417"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PLT35_14040"
     CDS             complement(<1..1006)
                     /locus_tag="PLT35_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30393.1"
                     /translation="MTEPSRYIFHVSTDEVARLAPAVLPATEGAPPVPPTAAHEGSPA
                     SLAAALRSLAAPSAAAVEAALILDERLVQSRVLAVPPLAPRHRQAAVELAFEPFMAEP
                     LEERRVVWTLLEEAADGAAPGADLTVAAWAAPRAWLEELLAAFAAARWQAEWIVPEAA
                     LWLPRLEELAAERGPTLLAIRRASSTLLLLGQAGRPPAWTTASDESDLRRAARQMTAL
                     LAGRPLSCLAWQGEDGGEPSEALRSLAPQAAAERLAPDVAAILEEAAAGSRPLPNFRS
                     GPLASGRARQRLVRHALAAGGLLLAGLVLWTAANFWQAHRLREASACSAAAQAALWQE
                     L"
     gene            complement(1003..1428)
                     /locus_tag="PLT35_14045"
     CDS             complement(1003..1428)
                     /locus_tag="PLT35_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30394.1"
                     /translation="MYAVAAAVLLAGAAQAVRAWRLAPGAPPAVDIKQLQQKAPPPAA
                     PRAETFADIVGRNFRQPHQEPAQPKAPPPPEKIKVKVVGLMIEPPNSCAVLTDDKGQR
                     AVRGVGDTFAEMKILAIDETGVTVERGGEKLRLEVEKPQ"
     gene            complement(1454..>1846)
                     /locus_tag="PLT35_14050"
     CDS             complement(1454..>1846)
                     /locus_tag="PLT35_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOG30395.1"
                     /translation="RTVGSILSGDARGPLNVNTASEAVLKFLGEAFDRGPLIDDIVRE
                     RQAAPFESLADLRRRLSMAADAETAMLSLFSYETMVYSAVIAVRGDERTQYFRAVLVR
                     RAPKDGDQENGVVEVSFIQPLARCQVAF"
BASE COUNT          260 a          678 c          679 g          229 t
ORIGIN      
        1 aaagctcctg ccagagcgcc gcctgggcgg cggctgagca ggccgaggcc tcgcggagcc
       61 ggtgggcctg ccagaagttg gccgcggtcc agaggaccag tcccgccagc agcagcccgc
      121 cggcggccag ggcgtggcgg acaagtctct gtcgcgcccg gcccgaggcc agcggccccg
      181 agcggaagtt gggcaatggc cggcttcccg cggcggcctc ttcgaggatg gcggccacgt
      241 ccggcgccag acgctcggcg gcggcctgcg gcgcgaggct acgcagggct tcgctcggct
      301 cgcccccgtc ctcgccctgc caggccaggc acgagagggg ccggccggcc aggagggccg
      361 tcatctgccg ggccgcccgt cgcaggtcgc tctcgtcgga ggccgtggtc cacgcaggcg
      421 ggcggcccgc ctgccccaga aggagcagcg tggacgacgc ccggcggatc gccaggagcg
      481 tcgggccgcg ctccgcggcc agttcctcca gccgcggcag ccagagcgcc gcctccggca
      541 cgatccactc cgcctgccag cgggcggccg cgaaggccgc caggagctcc tccagccagg
      601 cccgcggcgc ggcccaggcg gcgaccgtaa ggtccgcgcc gggggccgcc ccgtcggccg
      661 cctcttccag gagcgtccag acgacgcgcc gctcttccag cggctcggcc atgaacggct
      721 cgaaggccag ttccacggcc gcctggcggt gacgcggggc cagaggcggc acggccagga
      781 cgcgcgactg aacgagccgc tcgtcgagaa tgagggctgc ttctacggcc gcggcggaag
      841 gggccgcaag cgaccgcagg gccgcagcca gcgaggccgg cgagccctcg tgggcggcgg
      901 tcggcgggac gggcggtgcg ccctcggttg ccggcaggac ggctggggcc aaacgggcga
      961 cttcgtccgt cgagacgtgg aagatgtagc gcgagggttc ggtcactggg gcttctcgac
     1021 ctccagacgg agcttctcgc cgccgcgctc caccgtcacg cccgtctcgt cgatggccag
     1081 tatcttcatc tcggcgaaag tgtcgccgac gccccgcacg gcccgctggc ccttgtcgtc
     1141 ggtcaggacg gcgcagctgt tgggcggctc gatcatcagg cccacgacct tgaccttgat
     1201 cttctccggc ggcggagggg ccttcggctg ggccggttcc tggtgcggct ggcggaagtt
     1261 gcgcccgacg atgtcggcga aggtctcggc ccggggcgcc gccggcggcg gcgccttctg
     1321 ctgaagctgc ttgatgtcca cggccggcgg cgcgcccgga gcgagccgcc aggcgcggac
     1381 cgcctgcgcc gccccggcca ggagcacggc ggcggccacg gcgtacatgg gaacgagcgt
     1441 cagggccgtc cgcctagaag gccacctgac accgcgccag aggctggata aacgaaacct
     1501 cgacaacgcc attctcctgg tcgccgtcct tcggcgccct gcggaccagc acggcccgga
     1561 aatactgcgt ccgctcgtcg ccccggacgg cgatgacggc actataaacc atcgtctcat
     1621 aagaaaacag tgacaacatg gccgtctcgg cgtccgcggc catgctgagc cgccggcgca
     1681 ggtcggccag ggactcaaag ggcgccgcct gccgctcccg cacgatgtca tcgataagcg
     1741 gcccgcggtc gaaggcctcg ccgaggaact tcagaacggc ctcggaagcc gtattgacgt
     1801 tgagcggccc gcgggcgtcg ccgctcagga tcgaccccac ggttcg
//
LOCUS       DAOSIF010000580         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_54635_length_1845_cov_3.556983, whole genome shotgun sequence.
ACCESSION   DAOSIF010000580 DAOSIF010000000
VERSION     DAOSIF010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54635_length_1845_cov_3.556983"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLT35_14055"
     CDS             complement(<1..277)
                     /locus_tag="PLT35_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOG30396.1"
                     /translation="MDAIDLKALYADLKKRMEQVIDHLRRDLAGVRTGRATINILDPV
                     HVDAYGSRMPLNQVATLSVPDPMTILAQPFDPSLLGTIEKAIRASDLG"
     gene            complement(309..1037)
                     /gene="pyrH"
                     /locus_tag="PLT35_14060"
     CDS             complement(309..1037)
                     /gene="pyrH"
                     /locus_tag="PLT35_14060"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOG30397.1"
                     /translation="MDQPPPAPAYRRVLVKLSGEALMGEQGYGIDPALVGGIAREIGE
                     IRALGVDLAIVIGGGNIFRGVAAGAAGMDRAQADYMGMLATVINALALQDALENAGVS
                     TRVLTAIEMRAVAEPFIRRRAVRHLEKGRVVVFAAGTGNPYFTTDTAAALRAMEIKAD
                     VILKATKVDGIYSADPVSHPDATRFELLTYLDVLERRLKVMDATAISLCMDNKLPIIV
                     FNLRRPGNMRRAVLGERIGTVVRA"
     gene            complement(1056..1655)
                     /gene="tsf"
                     /locus_tag="PLT35_14065"
     CDS             complement(1056..1655)
                     /gene="tsf"
                     /locus_tag="PLT35_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOG30398.1"
                     /translation="MAITAAQVKALREKTGAGMMECKAALTETGGDMDEAVTVLRKRG
                     LAQAAKRAGRATSQGLVGSYIHMGGKIGVLVEVNCESDFVARTEGFQTLVKEVAMHIA
                     AADPKYLRREDVPAEVLDGERAIYRAQFEGSGKPAAVVDKIVEGKLGAFFSQAVLLDQ
                     PSVRDQAITVGEMVKQAIAKTGENIAIARFARFKLGEGA"
BASE COUNT          245 a          618 c          677 g          305 t
ORIGIN      
        1 ggccgaggtc cgacgcccgg atggccttct cgatcgtgcc gagcaggctc ggatcgaagg
       61 gctgggcgag aatcgtcatc ggatcgggca ccgacagcgt ggccacctgg ttcaggggca
      121 tccgcgatcc gtacgcgtcc acgtgcaccg gatcgaggat gttgatggtc gcccgcccgg
      181 tgcggacgcc ggcgaggtcg cgccgcaggt ggtcgatcac ctgctccatc cgtttcttga
      241 ggtctgcgta cagcgccttg agatcgatcg cgtccatgct ggtctgctcc ggatgctgtg
      301 gtctcgcgct acgcccggac gacggtcccg atccgctcgc ccaggacggc ccgcctcatg
      361 ttgccgggcc ggcgcaggtt gaagacgatg atggggagct tgttgtccat gcagagcgag
      421 atggccgtgg cgtccatcac cttcaggcgt cgctcgagca cgtcgaggta cgtgaggagc
      481 tcgaaccgcg tggcgtcggg gtgggagacc gggtcggcgg agtagatgcc gtcgaccttc
      541 gtggccttca ggatgacgtc ggccttgatc tccatcgccc gcagggccgc cgcggtgtcg
      601 gtcgtgaagt aggggttgcc cgtgccggcg gcgaacacca cgacgcgccc cttttcgagg
      661 tgccggacgg cgcggcgccg gatgaagggc tcggcgacgg ctcgcatctc gatggctgtc
      721 aggacgcggg tggaaacgcc ggcgttctcg agggcgtcct ggagggcgag ggcgttgatg
      781 accgtggcga gcatgcccat gtagtcggcc tgcgcccggt ccatgcccgc cgcgccggcg
      841 gcgacgccgc ggaagatgtt gccgccgccg atgacgatgg ccagatccac gccgagggcg
      901 cggatctccc cgatctccct ggcgatgccg ccgaccaggg caggatcgat gccgtagccc
      961 tgttcgccca tcagggcttc ccccgacagc ttgacgagga cgcggcggta ggctggcgca
     1021 ggcggcggct ggtccatacg ccgagggatt atagcctagg cgccctctcc cagcttgaac
     1081 cgcgcgaagc gagcgatggc gatgttctcg ccggtcttcg cgatggcctg cttgaccatc
     1141 tcgccgacgg tgatcgcctg atcgcggacc gagggctgat ccaggagcac cgcctggctg
     1201 aagaacgcgc cgagcttgcc ctcgacgatc ttgtcgacga ccgcggccgg cttgccggat
     1261 ccctcgaact gcgcgcggta gattgcgcgc tccccgtcga gcacctcggc gggcacgtcc
     1321 tcgcgacgca ggtacttcgg gtcggccgcg gcgatgtgca tcgcgacttc cttgacgagc
     1381 gtctggaacc cttcggtgcg ggcgacgaag tcggactcgc agttgacctc gacgaggacg
     1441 ccgatcttgc cgcccatgtg gatgtagctc cccacgaggc cctgcgaggt cgcgcggccc
     1501 gcgcgcttcg ccgcctgggc aagcccgcgc ttgcgcagga cggtgacggc ctcgtccatg
     1561 tcgccgcccg tctcggtcag ggcggccttg cattccatca tcccggcgcc ggtcttctcg
     1621 cgcagggcct tgacttgcgc tgccgtgatt gccatggctg tgcgatctcc gcggtacgaa
     1681 aaccgcgccg gcgcgggacg ggcccggccg gcgcgaaggg gtgctgccgg ggccgatcgg
     1741 ccccggtcgt caggtcttca ggccgacgcg ggcgccgctg ccggcggcgc gtccggccgg
     1801 gaccggcccg gggccgggcg cgcgccgtgg gcgaggagga gggtg
//
LOCUS       DAOSIF010000581         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_54758_length_1842_cov_3.494124, whole genome shotgun sequence.
ACCESSION   DAOSIF010000581 DAOSIF010000000
VERSION     DAOSIF010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54758_length_1842_cov_3.494124"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLT35_14070"
     CDS             <1..510
                     /locus_tag="PLT35_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30399.1"
                     /translation="GWLEPGAFAFTWVTDFPLLEWDSEERRWVAMHHPFTSPVEAHID
                     RLEEEPGKALAQAYDLVLNGSEIGGGSIRIHDTGLQRRMFSLLGISEEQARLRFGFFL
                     EALEYGTPPHGGIALGLDRIVAILAGEPSIRDVIAFPKTAQAVDLMSSAPAPVDDRQL
                     RELKLRIGD"
     gene            514..1521
                     /locus_tag="PLT35_14075"
     CDS             514..1521
                     /locus_tag="PLT35_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG30400.1"
                     /translation="MDAKAPGPATRTISLPDEGVEALFGAYDENLRFIESLANVRLST
                     HGPDLVVSGDPLAVARVEHLVGRLLALQKDGYRLTNGDVKTAADLLAQDPSADLRDFF
                     LKSALRPGGGRRTVNARSLNQRRYIEAIDRADIVFGIGPAGTGKTYLAMAQAVSYLLA
                     KRVSRIVLARPAVEAGEKLGFLPGDLQEKVNPYLRPLYDALYDMMDGERADRLIERGT
                     IEIAPLAFMRGRTLGDAFVILDEAQNTSSEQMKMFLTRLGLGSKAVVTGDITQIDLPA
                     GRTSGLIEAIGVVGGVEGVEFVSFDERDVVRHKVVQLIVRAYEAYSSRGPGARGQESP
                     A"
     gene            1518..>1842
                     /gene="ybeY"
                     /locus_tag="PLT35_14080"
     CDS             1518..>1842
                     /gene="ybeY"
                     /locus_tag="PLT35_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG30401.1"
                     /translation="MSARSGSAPGRRGVRVTVASRMRRPVRAPGLAAWLASAAPAFAR
                     GEVVVAFVSDAHIRDLNRRYRGKDAVTDVLAFPSDEPGLLGDIAIAAGAARRQAAAAG
                     HAFGVE"
BASE COUNT          269 a          625 c          680 g          268 t
ORIGIN      
        1 ggctggctcg agccgggcgc gttcgcgttc acgtgggtga ccgacttccc gctcctcgag
       61 tgggactccg aggagaggcg gtgggtggcc atgcaccacc cgttcacgtc gcccgtcgag
      121 gcccacatcg accgtctgga agaagagccc ggcaaggccc tggcgcaagc ctacgatctc
      181 gtgctgaacg gcagcgagat cggcggcggc agcatccgca tccacgacac gggcctccag
      241 cgccgcatgt tctccctgct cggcatcagc gaggagcagg ccaggctgcg cttcggcttc
      301 ttcctcgaag cgctcgagta cgggacgccg ccgcacgggg ggatcgcgct cggcttggat
      361 cggatcgtgg ccattctcgc cggcgagcct tcgattcggg acgtcatcgc gttcccgaag
      421 acggcgcagg cagtggactt gatgtcgtcg gcgccggcgc ccgtcgacga caggcagttg
      481 cgcgagctga agctccgcat cggggactga gggatggacg cgaaggcccc gggaccggcg
      541 acgcggacga tatcgctgcc cgacgaaggg gtggaggcgc tcttcggcgc gtacgacgag
      601 aacctgcgct tcatcgagtc gctggccaac gtccggctct cgacgcacgg gccggatctc
      661 gtcgtgtcgg gagacccgtt ggccgtcgcg cgcgtcgagc acctcgtcgg gcggctgctg
      721 gcgctgcaga aggacggcta ccggctgacg aacggggacg tcaagacggc ggccgacctg
      781 ctcgcgcagg acccgtccgc ggacctccgc gacttcttcc tgaagtccgc gctgcgcccg
      841 ggaggcggcc gacggacggt gaatgcccgc agcctcaacc agcggcgcta catcgaggcc
      901 atcgaccgtg cggacatcgt cttcggcatc ggcccggccg gcacgggcaa gacctacctg
      961 gcaatggccc aggcggtctc gtacctgctg gcgaagcgcg tgagccgcat cgtgctggcg
     1021 cggccggccg tcgaggcggg cgagaagctg ggcttcctgc cgggcgattt gcaggagaag
     1081 gtgaacccct atctcaggcc gctctacgac gcgctctacg acatgatgga cggcgaacgg
     1141 gcggatcgct tgatcgagcg cggcacaatc gagattgcgc cgctggcgtt catgcgcggg
     1201 cgcacgctcg gcgacgcctt cgtcatcctc gacgaggccc agaacacgag cagcgagcag
     1261 atgaagatgt tcctcacgcg cctgggcctg ggatccaagg ccgtggtcac cggcgacatc
     1321 acgcagatcg acctgccggc cggcaggacg tcgggcctca tcgaggccat tggcgtcgtc
     1381 ggcggcgtcg agggcgtcga attcgtctcc ttcgacgagc gcgacgtcgt gcggcacaag
     1441 gtcgtgcagc tgattgtccg ggcgtacgag gcgtactcct cgcgcgggcc cggcgcgagg
     1501 ggccaggaga gcccggcatg agcgcgcgca gcggctcggc gccgggccgg cgcggcgtgc
     1561 gtgtcaccgt ggccagccgg atgcgccgcc cggtgcgggc gccgggcctc gcggcctggc
     1621 tggcctcggc ggccccggcc ttcgcgcggg gcgaggtggt ggtggcgttt gtcagtgacg
     1681 cgcacatccg ggacctcaac cggcgctacc gggggaagga cgccgtgacc gacgtgctcg
     1741 cgttcccctc ggacgaaccc ggcctgctcg gcgacatcgc cattgccgcg ggggcggcca
     1801 ggcggcaggc cgccgcggcc ggccatgcct tcggcgttga ag
//
LOCUS       DAOSIF010000582         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_54842_length_1840_cov_3.295798, whole genome shotgun sequence.
ACCESSION   DAOSIF010000582 DAOSIF010000000
VERSION     DAOSIF010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54842_length_1840_cov_3.295798"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="pilM"
                     /locus_tag="PLT35_14085"
     CDS             <1..680
                     /gene="pilM"
                     /locus_tag="PLT35_14085"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOG30402.1"
                     /translation="GEAAAPNSMDVLLVAAKMEKIADYNGVIAQADRVPVVVDVDAFA
                     IQNAFEANYDADPGTVTALMNMGASAININIVSGPATIFTRDVPIGGNAYTEAIQREF
                     NLTFDRAEQVKRGAGAGDERADEVEAVLRAVSDNVLLEIQRTFDFYRSTAASDRLDRV
                     MLCGGGARVRGFADALADRFGLPVEPLDPFRRIAFDAPAFGVDAVETAPVAVVAVGLA
                     LRRAGDR"
     gene            677..1258
                     /locus_tag="PLT35_14090"
     CDS             677..1258
                     /locus_tag="PLT35_14090"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOG30403.1"
                     /translation="MIRINLLTQEREKPRRQAAGPLVGSGQKITVACTLILALAALGV
                     GWWYWSLRKEESKLKSDIATAQAETARLQALIVQVRQFEERKAQLQQRVALIEDLRRS
                     QSSPVHVLDAISRSVPDSLWLTALDQKGRNLTLEGRCLSMTSISDFVDNLGRTGWFEK
                     PVEIVDSQVENGQAAGADVIKFTVRAQVTTPAG"
     gene            1277..>1840
                     /gene="pilO"
                     /locus_tag="PLT35_14095"
     CDS             1277..>1840
                     /gene="pilO"
                     /locus_tag="PLT35_14095"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOG30404.1"
                     /translation="MDLNLHKLPWYAQVGLFVALAVAGVAAFYYLYAMPADKALDAQR
                     QRLATLRVDISRGMAIARQLPQFRAQVADLEGRLEGLKAILPDEKDFGELLRNLQTLA
                     VQSNLSIRVFKPAPVVTQPLHAEWPINIELDGTYHNLGLFFERISRFPRIINVGNISV
                     KGKDKPDQNTSISVACVATTFVLLDGAQ"
BASE COUNT          310 a          641 c          622 g          267 t
ORIGIN      
        1 gcggcgaggc cgcggcgccg aactcgatgg acgtcctgct ggtggcggcg aagatggaga
       61 agatcgccga ctacaacggc gtgatcgctc aggccgaccg ggtccccgtc gtcgtcgacg
      121 tggacgcctt cgcaatccag aacgccttcg aggcgaacta cgacgccgac cccggcaccg
      181 tcaccgcgct catgaacatg ggcgccagcg cgatcaacat caacatcgtc agcgggccgg
      241 cgacgatctt cacgcgcgac gtcccgatcg gcggcaacgc ctataccgag gccatccagc
      301 gcgagttcaa cctgaccttc gaccgcgccg agcaggtcaa gcgcggcgcg ggcgccggcg
      361 acgagcgcgc cgacgaggtc gaagcggtcc tgcgcgccgt cagcgacaac gtcctgcttg
      421 agattcagag gaccttcgac ttctaccggt ccacggccgc ctcggatcgg ctcgaccgcg
      481 tgatgctgtg cggcggcggc gcccgcgtgc gcgggttcgc cgacgcgctc gccgaccgct
      541 tcgggctccc tgtcgagcca ctcgacccct tccggcggat cgccttcgac gcgcccgcct
      601 tcggcgtcga cgccgtcgag accgcgcctg tcgccgtcgt ggcggtgggc ctcgcactgc
      661 gacgggcagg tgaccgatga tccgcatcaa cctcctcacc caggagcggg agaagccgcg
      721 gcgacaggcc gcgggcccgc tcgtcggctc gggccagaag atcaccgtcg cctgcaccct
      781 catcctcgct ctggccgccc tcggcgtggg ctggtggtac tggtcgctgc ggaaggaaga
      841 gtcgaagctc aagtccgaca tcgcgaccgc ccaggcggag accgccaggc tccaggcgct
      901 catcgtgcag gtgcggcagt tcgaggaacg caaggcccag ctgcagcagc gcgtggcgct
      961 catcgaggac ctccggcgca gccagagcag ccccgtgcac gtgctggatg cgatcagccg
     1021 cagcgtcccg gactccctgt ggctgacggc gctcgaccag aaaggccgga acctcacgct
     1081 ggagggccgc tgcctgtcga tgacgtcgat ttcggacttc gtggacaacc tcgggcggac
     1141 gggctggttc gagaagcccg tggaaatcgt ggacagccag gtcgaaaacg gccaggcggc
     1201 aggcgccgac gtcatcaagt tcactgtccg ggcccaggtg acgacgccgg caggctagcg
     1261 tggaagcggg atcgcgatgg atctgaacct gcacaaactc ccgtggtacg cccaggtggg
     1321 gctgtttgtc gcgctggcgg tcgccggcgt ggcggccttc tactacctgt acgccatgcc
     1381 ggccgacaag gccctcgacg cccagcgcca gaggctggcc accctgcggg tggacatcag
     1441 ccggggcatg gccatcgcgc ggcagctccc gcagttccgg gcgcaggtcg ccgatctcga
     1501 ggggcggctg gagggcctca aggccatcct gcccgacgag aaggacttcg gcgagctgct
     1561 gcggaacctg cagacgcttg ccgtgcagtc gaacctgagc atacgcgtgt tcaagccggc
     1621 gccggtggtg acccagccgc ttcacgcgga gtggcccatc aacatcgagc tcgacggcac
     1681 ctaccacaac ctcgggctct tcttcgaacg gatcagccgc tttccgcgga tcatcaacgt
     1741 cggcaacatc tcggtgaagg gcaaggacaa gcccgatcag aacacgagca tctcggtggc
     1801 ctgcgtggcg acgacgttcg tcctgctgga cggcgcacag
//
LOCUS       DAOSIF010000583         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_55122_length_1833_cov_3.695163, whole genome shotgun sequence.
ACCESSION   DAOSIF010000583 DAOSIF010000000
VERSION     DAOSIF010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55122_length_1833_cov_3.695163"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLT35_14100"
     CDS             <1..620
                     /locus_tag="PLT35_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOG30405.1"
                     /translation="PRHATVDVRSVSGDIKLTAVDGSSRAEAVSGDVVVAKAPQLEAA
                     KTVSGDVTIDEAGSGGDLSVGSVSGDVRVRAVRVRSLDANIVSGKLHLLNVACERLKA
                     NSISGSLVFDGPLAKGGRYRFRSHSGDVTIYVDGKSGFELNASTFSGDITTGVELTPT
                     AGAEGDRPGRTPTPGTGRRVRGTLGDGGALIEINAFSGGVKIVKR"
     gene            complement(628..>1833)
                     /locus_tag="PLT35_14105"
     CDS             complement(628..>1833)
                     /locus_tag="PLT35_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG30406.1"
                     /translation="TQWFRRRVRDSYREVRGWIARINAFLQENITGMATVQLFRRESV
                     NGARFGAINARHRDANIQSIFYYAVFYPAIEIVGAAATALLIWQGGVWVLGGSLTLGS
                     LVAFIQYSRRFFRPISDLSEKFNLLQSAMAASERIFALLDTPVRVAPPAAPRAAAPEG
                     RVAFDGVSFAYDGERDVLRDVSFEVQPGQRVGIVGATGAGKSTIINLLLRFYDVRRGR
                     ILVDGVDIRDMDLGELRRRFSLVLQDVHLFSGTIGGNVRMGDATIDNARVRRALEAVH
                     ADRFVDRLPQGIDTLVAERGATLSTGQKQLLSFARALAFDPRILVLDEATSSIDTETE
                     VLIRDALRVLMAGRTTIAIAHRLSTIQDMDRILVLHKGRLREAGSHQELLAQRGIYYR
                     LYQLQYAGG"
BASE COUNT          287 a          629 c          660 g          257 t
ORIGIN      
        1 tgccccggca tgcgacggtg gacgtgcggt cggtttccgg cgacatcaag ctgacggcgg
       61 tcgacgggtc gtcgagggcc gaggccgtca gcggggacgt cgtggtcgcg aaggcgcccc
      121 agctcgaggc ggcgaagacg gtgtccgggg acgtcacgat cgacgaggcg ggcagcggcg
      181 gcgatctctc ggtgggcagc gtgagcggcg acgtacgggt ccgggccgtc agggtccgga
      241 gcctcgacgc gaacatcgtg agcggcaagc ttcatctgtt gaacgtggcg tgcgagcggc
      301 tgaaagccaa ctcgatcagc ggcagtctgg tgttcgacgg gcccctcgcg aagggcggcc
      361 ggtaccgctt ccggtcgcac tcgggcgacg tgacgatcta cgtcgacggc aagtcgggct
      421 tcgagctgaa cgccagcacg ttcagcggcg acatcacgac cggcgtggag ctcacgccga
      481 cggccggggc cgaaggcgac cggcccggcc ggacgccgac acccggcacc ggccgccgcg
      541 tgcgcggcac gctcggcgac ggcggggcgt tgatcgagat caacgcgttc agcggcggcg
      601 tgaagatcgt caagcggtag cggcccgtca cccgccggcg tactgcagct ggtagagccg
      661 gtagtagatc ccgcgctgcg cgagcagctc ctggtgcgag cctgcctcgc gcagccggcc
      721 tttgtgcagg accaggatcc ggtccatgtc ctggatggtc gagagccggt gggcgatggc
      781 gatcgtggtc cggcccgcca tcaggacgcg cagcgcatcg cggatgagca cctcggtctc
      841 ggtgtcgatg ctggaggtcg cctcgtccag cacgaggatc cgcgggtcga acgccagggc
      901 ccgcgcgaac gacagcagct gcttctgccc cgtcgacagc gtcgcgcccc gctcggcgac
      961 cagggtgtcg atcccctgcg gcagccggtc cacgaaccgg tcggcgtgga cggcttcgag
     1021 ggcccttcgc acccgggcat tgtcgatcgt cgcgtcgccc atcctcacgt ttccgccgat
     1081 ggtccctgag aagagatgca cgtcctgcag gaccaggctg aaccgccggc ggagctcgcc
     1141 gaggtccatg tcgcggatgt ccactccgtc cacgaggatg cggccgcggc ggacgtcgta
     1201 gaaccgcagc agcaggttga tgatcgtgga cttgcccgcg cccgtcgcgc ccacgatgcc
     1261 gacgcgctgg cccggctgca cctcgaagga cacgtcccgg aggacgtcgc gctcgccgtc
     1321 gtaggcgaac gacacgccgt cgaacgccac tcgcccctcc ggcgccgcgg cgcgcggcgc
     1381 ggccggcggg gccacccgca cgggcgtgtc gagcagcgcg aagatccgct ccgaggcggc
     1441 catcgcggac tgcaggagat tgaacttctc cgacaggtcg ctgatcggcc tgaagaaccg
     1501 cctcgagtac tggatgaacg ccacgagcga cccgagcgtc aggctccccc caaggaccca
     1561 gactccgccc tgccagatca gcagggccgt ggcggccgcc ccgacgatct cgatcgccgg
     1621 atagaacacg gcgtagtaga agatcgattg gatgttcgcg tcgcggtggc gggcgttgat
     1681 ggcgccgaac cgcgcgccgt tcaccgactc gcgcctgaac agctgcaccg tcgccatgcc
     1741 cgtgatgttc tcctggagga acgcgttgat gcgcgcgatc cagccccgaa cctcccggta
     1801 ggagtcgcgc acgcgccggc ggaaccactg cgt
//
LOCUS       DAOSIF010000584         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_55243_length_1830_cov_3.292958, whole genome shotgun sequence.
ACCESSION   DAOSIF010000584 DAOSIF010000000
VERSION     DAOSIF010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55243_length_1830_cov_3.292958"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLT35_14110"
     CDS             <1..793
                     /locus_tag="PLT35_14110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG30407.1"
                     /translation="YGCTFCGAKTTSTRKFRARSPENVIAEMEEVYRRFGIPNFFISD
                     DLFTLKRQRVLDICALILEKKLPITWTCLSRVNTIDAEMLALMKRAGCYLISYGLESG
                     SQAVLDKLKKGTTVEQGIQAVEMTKAAGIKVFGSFMIGSPGDTPETVEETIALIRRLK
                     LDEVGLGVTTAYPGTDLFDAFGSDAKGLDWDKALAFNPSAADRSDVFLKCTDLDDEQI
                     RRLFHKAMRQSVLYNPRLLVRRLSHIASVRHLGQSARAAARIFWG"
     gene            complement(790..>1830)
                     /locus_tag="PLT35_14115"
     CDS             complement(790..>1830)
                     /locus_tag="PLT35_14115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG30408.1"
                     /translation="TWVCDARCEMCSNWKRGNRKLDMTLEQIDGVFRSDFWRNIENAA
                     ISGGEPTTRNDLVEICELMIDRFPRLRKFGLNTTGLTPHRAIPMIEKIAAACQRKGVI
                     FAMRVSIDGVGDMHNLVRNVKNGFDKAYETIRAVREMQRRYRFNYGVSTTIFSMNLND
                     AENILAWARREKLDIVFNMIRFTEPALGNADLANAHKPVGEAEERMRQFFLDRVRMDP
                     LMDGQNYVYMHYADMIANGYHRLAPCPFQSQGIMLNPDGALHFCENSRAVGNCLETDP
                     GELYFRAESQAHRDEIRDTVCPTCLSPCQMNVAAVKQVVPYVKFLARATAEKRRSRRL
                     QPPGSPGRRGPA"
BASE COUNT          285 a          598 c          617 g          330 t
ORIGIN      
        1 gtacgggtgc accttctgcg gcgcgaagac cacgtcgacg cggaagttcc gcgcccggag
       61 ccccgagaac gtcatcgccg agatggagga ggtctaccgg aggttcggca tcccgaattt
      121 cttcatctcg gacgacttgt tcacgctgaa gcggcagcgc gtgctcgaca tctgcgcgct
      181 gatcctggag aagaaactgc cgatcacctg gacgtgcctc tcgcgggtga ataccatcga
      241 cgccgagatg ctggccctga tgaagcgggc gggctgctac ctgatttcgt acggcctcga
      301 gtcgggttcg caggccgtgc tcgacaagct caagaaaggc acgacggtcg aacagggcat
      361 ccaggccgtc gagatgacga aggctgcggg catcaaggtg ttcggctcgt tcatgatcgg
      421 ttcgccgggg gacacgcccg aaacggtcga ggagacgatc gcgctgatcc gccgtctgaa
      481 gctggacgag gtgggcctcg gcgtgacgac cgcgtacccg ggtaccgact tgttcgacgc
      541 cttcggctcg gacgcgaagg ggctcgattg ggacaaggcg ctggcgttca atccgtcggc
      601 ggccgaccgc agcgacgtgt tcctgaagtg caccgacctg gacgacgagc agatccgccg
      661 gctcttccac aaggcgatgc ggcagtcggt gctctacaac ccgaggctgc tcgtccggcg
      721 gctgtcgcac atcgcgtcgg tgcgccacct cggccagtcg gccagggccg ccgcgcggat
      781 cttctggggc taggccgggc cccgccgtcc cggcgagcct ggcggctgga gccgccggct
      841 ccggcgcttc tcggcggtcg cgcgcgcgag gaacttcacg tacggcacca cctgcttcac
      901 cgcggcgacg ttcatctggc acgggctcag gcacgtcggg cacaccgtgt cccggatctc
      961 gtcgcggtgc gcctggctct cggcccggaa gtacagttcg cccggatccg tctcgaggca
     1021 gttgccgacg gcgcggctgt tctcgcagaa atggagcgcg ccgtccgggt tcagcatgat
     1081 cccctgggac tggaacgggc acggcgcgag ccggtggtac ccgttggcga tcatgtccgc
     1141 gtagtgcatg tagacgtagt tctgcccgtc catcagcgga tccatccgga cgcggtccag
     1201 gaagaactgc cgcatccgct cctcggcctc gccgacgggc ttgtgggcgt tcgccaggtc
     1261 cgcgttgccg agggccggct cggtgaagcg aatcatgttg aagacgatgt ccagcttctc
     1321 ccggcgcgcc cacgcgagga tgttctcggc gtcgttcagg ttcatcgaga agattgtcgt
     1381 cgacacgccg tagttgaacc ggtaccggcg ctgcatctcc cggacggccc ggatcgtctc
     1441 gtacgccttg tcgaagccgt tcttcacgtt gcggacgagg ttgtgcatgt cgccgacgcc
     1501 gtcgatcgac acgcgcatgg cgaagatgac gcccttgcgc tggcaggccg cggcgatctt
     1561 ctcgatcatc gggatcgccc ggtgcggcgt cagcccggtc gtgttgaggc cgaacttcct
     1621 caggcgcggg aaccggtcga tcatcagctc gcagatctcg accagatcgt tccgcgtcgt
     1681 cggctcgccg ccggagatgg cggcgttctc gatgttccgc cagaagtcgg accggaagac
     1741 gccgtcgatc tgctcgagcg tcatgtcgag cttccggttg ccccgcttcc agttgctgca
     1801 catctcgcac cgggcgtcgc acacccaggt
//
LOCUS       DAOSIF010000585         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_55874_length_1815_cov_3.617045, whole genome shotgun sequence.
ACCESSION   DAOSIF010000585 DAOSIF010000000
VERSION     DAOSIF010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55874_length_1815_cov_3.617045"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /gene="ppk1"
                     /locus_tag="PLT35_14120"
     CDS             <1..1321
                     /gene="ppk1"
                     /locus_tag="PLT35_14120"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOG30409.1"
                     /translation="GAPGESMYRVPGLLDLRALWSLVELPGFEELRDPGMRPVPVLED
                     GPAGRIFDALDGADILVHHPYDSFDAVLALVEQAADDPDVLAIKQTLYRPGGSDSPVI
                     AALMRAARHGKQVTAVVELMARFDEESNLRWARALEEAGAHVIYGVRGLKVHAKCCLV
                     VRRTDQGLRRYVHVATGNYNHRTARVYTDVGLLTSSPAFGQDASAFFNALTGYSDPPR
                     MKTLVLAPSQLRDRILRLIERETRRAGEGQPALIRAKMNALVDERVVEALYRASDAGV
                     RVQLNVRGICVLRPGVAGLSENIQVVSIVGRFLEHARVFHFHNGGEDEVYISSADWMP
                     RNLDRRVELMAPVEAKPCRRRLLHALDVLFQDNVKARRLFPDGTWRVPPRPASAQPFV
                     AQAALYEHARRAWARRQASPAESFAPIDRGPDGHHAQAPGRRAALNT"
     gene            complement(1311..>1815)
                     /locus_tag="PLT35_14125"
     CDS             complement(1311..>1815)
                     /locus_tag="PLT35_14125"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG30410.1"
                     /translation="PTLETAIVYPGAVLVEGTQVSEGRGTTRPFELLGAPWVDAERVS
                     ADLNGLGLGGVRFRPAVFEPTFHKFARESCGGCQIHVTDRRAFRPVLAAVAVLAAFRR
                     AGPDRFAWRQPPYEYEHEKMPIDILAGSPALREQIESGVPAREIAASWDGPVSAFESI
                     RRPRLMY"
BASE COUNT          250 a          677 c          650 g          238 t
ORIGIN      
        1 cggcgccccc ggggagtcga tgtaccgcgt gccggggctg ctcgacctgc gcgccctgtg
       61 gagcctggtc gagctgcccg ggttcgagga gctccgggac cccggcatga ggcccgtgcc
      121 cgtgctcgag gacggcccag ccgggcgcat cttcgacgcc ctggacggcg ccgacatcct
      181 cgtgcaccac ccgtacgact ccttcgacgc cgtcctcgcc ctcgtcgagc aggccgcgga
      241 cgatcccgac gtgcttgcca tcaagcagac cctctaccgg ccgggcggtt cggactcgcc
      301 cgtcattgcg gccctcatgc gagcggcccg ccacggcaag caggtgacgg ccgtcgtgga
      361 gctgatggcc cgcttcgacg aggagagcaa cctccggtgg gcgcgggcgc tcgaggaggc
      421 cggcgcgcac gtgatctacg gcgtccgcgg gctgaaggtc cacgccaagt gctgcctcgt
      481 cgtgcgccgt acggaccagg gcctccggcg ctacgtccac gtggcgaccg ggaactacaa
      541 ccaccgcacc gcgcgggtct acacggacgt cggcctgctc acgtcctcgc ccgcgttcgg
      601 gcaggacgcg tcggcgttct tcaacgccct gaccggctat tccgatccgc cgcggatgaa
      661 gacgctcgtg ctggcgccgt cccagctgcg cgaccggatc ctcaggctga tcgagcggga
      721 gacgcggcgg gcgggcgagg ggcagccggc gctcatccgg gcgaagatga acgcgctggt
      781 cgacgagcgc gtcgtcgagg cgctctaccg cgcgtcggac gccggcgtgc gcgtgcagct
      841 gaacgtgcgc ggcatctgcg tgctgcggcc cggcgtcgcc ggcttgagcg agaacatcca
      901 ggtcgtgtcg atcgtgggcc gtttcctcga gcacgcccgc gtcttccact tccacaacgg
      961 cggcgaggac gaggtctaca tctcgagcgc cgactggatg ccgaggaacc tggaccggcg
     1021 cgtcgagctg atggcgcccg tcgaggcgaa gccctgccgc aggcgcctgc tgcacgcgct
     1081 cgacgtgctg ttccaggaca acgtgaaggc gcggcgcctc tttcccgacg ggacgtggcg
     1141 cgtcccgccc cggcccgcgt ccgcgcagcc cttcgtggcc caggcggcgc tctacgagca
     1201 cgcgcgccgc gcgtgggccc ggcggcaggc gtcgcccgcc gagagcttcg cccccatcga
     1261 tcgcgggccg gacggccatc acgcgcaggc gccgggccga cgggcggcgc tcaatacatg
     1321 aggcgcgggc gcctgatcga ctcgaacgcc gacacggggc cgtcccacga cgcggcgatc
     1381 tcgcgcgcgg gaacgccgga ctcgatctgc tcccgcagcg ccggcgatcc ggccaggatg
     1441 tcgatgggca tcttctcgtg ctcgtactcg tacggcggct gccgccacgc gaacctgtcc
     1501 gggccggcgc ggcgaaacgc cgccagcacg gcgacggccg cgagcaccgg ccgaaaggcc
     1561 cggcgatcgg tcacgtggat ctggcacccg ccgcacgact cgcgggcgaa cttgtggaac
     1621 gtcggctcga acacggccgg ccggaagcgc acgccgccga ggccgaggcc gttcagatcg
     1681 gccgacacgc gctcggcatc cacccacggc gcgcccagca gctcgaacgg gcgcgtcgtg
     1741 ccgcgtccct ccgagacctg cgtgccctcg acaagcacgg cgcccgggta gacgatggcg
     1801 gtctcgagcg tgggc
//
LOCUS       DAOSIF010000586         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_55912_length_1814_cov_4.446845, whole genome shotgun sequence.
ACCESSION   DAOSIF010000586 DAOSIF010000000
VERSION     DAOSIF010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55912_length_1814_cov_4.446845"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLT35_14130"
     CDS             <1..760
                     /locus_tag="PLT35_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30411.1"
                     /translation="AGAEEPAETGPTAEEQEAAAHADAARAAQDRADADVASGDYAAA
                     AQERAIAEDEAWAAGDSSMLHGASSTNLDSAAAYHVEAERLEQEEARHARDGDYEAAR
                     DYAGRAADAQSWSDYRAGGADHTGQARAEYEKEDWAVWHQDQAAHAEYNAAWYAEHGN
                     PDAAASYAGLAADHQAQADQYGHEGEHGAVGAVHDASSEMAHDTPVGGDSSDGHSAGT
                     DDGGWADHAGDAGADYSADDSASYDSGSTDSGDA"
     gene            941..>1814
                     /locus_tag="PLT35_14135"
     CDS             941..>1814
                     /locus_tag="PLT35_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOG30412.1"
                     /translation="MRRLAVPCVLAALALAVPAAARAQAPSAAPSAAQLAAQLPAETP
                     QQKEARLQWWTDARFGMFIHWGLYALPARHEWVKKYERISDADYQKYFDQFDPDLYDP
                     REWARQAKQAGMKYAVITSKHHEGFCLFDSKYTDYKATRTPAKRDLIKAFVEAFRAEG
                     LKVGFYYSLLDWHHPSYTVDRNHPQSGSTDAEYAALNAGRDMDVYRQYVKDQVRELLT
                     NYGAIDIIWLDFSFAGGAHGKGRSDWDSVNLLKMVRELQPGIIVNDRLDLDDVPGGWD
                     FRTPEQFKPCGWVMV"
BASE COUNT          302 a          642 c          665 g          205 t
ORIGIN      
        1 ggccggagcc gaggagcccg ccgagaccgg gccgacggcg gaggagcagg aggccgcggc
       61 ccacgccgac gcggcgcggg ccgcccagga ccgggccgac gccgatgtgg cgagcggcga
      121 ctacgccgct gcggcccagg agcgggcgat cgccgaggac gaggcgtggg ccgccggcga
      181 cagcagcatg ctgcacggcg cgtcgtccac caacctggac tcggccgcgg cgtaccacgt
      241 cgaagccgag cgcctggagc aggaggaggc gcggcacgcc cgcgatggcg actacgaggc
      301 ggcgcgggac tacgcgggcc gcgcggccga cgcccagtcg tggtccgact accgcgcggg
      361 cggagcggac cataccgggc aggcgagggc cgagtacgag aaggaggact gggcggtctg
      421 gcaccaagac caggccgcgc acgccgaata caatgccgcc tggtacgccg agcacggcaa
      481 ccccgacgcg gcggcgagct acgccgggct cgctgcggat catcaggcgc aggcggacca
      541 gtacgggcac gagggcgagc acggcgccgt cggcgccgtc cacgacgcat cgtcggagat
      601 ggcccacgac acgccggtcg gcggcgactc gtccgacggc cactccgcgg ggacggacga
      661 cgggggctgg gcggatcatg ccggcgacgc gggagcggac tactcggccg acgacagcgc
      721 gagctacgac agcggaagca ccgattcggg cgacgcctga cgcagcggcg ggcgagcctc
      781 agggcggtgg tccggcgccg aaggcgcgcg tcgtgcgcgc accggcagcc gggccgcccg
      841 ccgtcctctc ggccgcgccg gtcatcgcct ggacggagcc gaaggcgtcg ccggctccga
      901 ggctccgcat ttgctatcat gcgcgccacg gaggatccct atgcgcagac tcgccgtgcc
      961 ctgtgtgctg gccgcgctcg cgctggccgt gcccgcggcc gcccgcgcgc aggcgccgtc
     1021 cgccgcgccg tcggcggcac aactggccgc gcagctgccg gccgagacgc cgcagcagaa
     1081 agaggcccgc ctgcagtggt ggaccgatgc gcgcttcggc atgttcatcc actggggcct
     1141 ctacgccctg ccggcgcgcc acgagtgggt gaagaagtac gagcggatca gcgacgccga
     1201 ctaccagaag tacttcgacc agttcgaccc ggacctctac gacccgcgcg aatgggctcg
     1261 ccaggcgaag caggccggca tgaagtacgc ggtgatcacg tcgaagcacc acgaggggtt
     1321 ctgcctgttc gactcgaagt acaccgacta caaggccacg cggacgcccg cgaagcgcga
     1381 cctcatcaag gccttcgtcg aggccttccg cgccgagggc ctgaaggtcg ggttctacta
     1441 ttcgctgctc gactggcacc atccgtccta caccgtcgac cgcaaccacc cgcagagcgg
     1501 atccaccgac gcggagtatg ccgcgctcaa cgcgggccgc gacatggacg tctaccgcca
     1561 gtacgtgaag gaccaggtgc gcgagctgct cacgaactac ggcgcgatcg acatcatctg
     1621 gctcgacttc tcgttcgccg gcggagcgca tggcaagggc cgctccgact gggactcggt
     1681 gaacctcctg aagatggtcc gcgagctgca gccgggcatc atcgtcaacg accggctcga
     1741 cctggatgac gtgccgggcg gctgggactt ccggacgccc gagcagttca agccctgcgg
     1801 ctgggtcatg gtga
//
LOCUS       DAOSIF010000587         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_56456_length_1801_cov_3.971363, whole genome shotgun sequence.
ACCESSION   DAOSIF010000587 DAOSIF010000000
VERSION     DAOSIF010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56456_length_1801_cov_3.971363"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1508)
                     /gene="mnmE"
                     /locus_tag="PLT35_14140"
     CDS             complement(72..1508)
                     /gene="mnmE"
                     /locus_tag="PLT35_14140"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG30413.1"
                     /translation="MYSVPVFSPADTIAALATAPVRAAVGVVRLSGPRARQCARAILT
                     LPRDLEPRRATVTRAARRREAPAGREGPGPAARAIDRVVATFFPAPQSYTGEDVVEIS
                     AHGSVALLREILQSAIDAGARLAEPGEFTLRAFLNGRMDLVQAEAVADLVDAVTPLQA
                     RVAFDQLEGTLTRAIAALDGELFDLTARLEASVDFPDEGFHFIEAGDAARVARRVIGG
                     IDALLATAARGRLVREGRQIAILGRPNVGKSSLFNWLVGADRAIVSSRPGTTRDMLTE
                     AIDLDGIRLGLVDTAGIRSSEDEVEREAVSRGRRTAAVADLVLLVLDRSRPLDETDRA
                     LLRDTAGAARIVVINKTDLAPAWSTESPDGLARIVGAGRAAAVSLKTGDGLEELRSAM
                     RAALEAGDIPRDGPVVANARHEALLRAAREPMARALANLEAGGEQASEELVLADLSAA
                     RRCLEEVTGRRTSEDVLKRIFERFCVGK"
BASE COUNT          237 a          698 c          623 g          243 t
ORIGIN      
        1 cgcccacgct gcctccacgc cggcgtggcc cgcgccgatc acgaggacat cgaaatcggg
       61 cacgcgcccc ctcacttccc cacgcagaac cgctcgaaga tccgcttcag cacatcctcc
      121 gacgtgcgcc tgcccgtgac ttcctcgagg caccgccgcg ccgccgacag gtccgccagc
      181 accagttcct cgctcgcctg ctcccctccc gcctccagat tcgccagcgc gcgggccatc
      241 ggctcccgcg ccgctctcag cagcgcctcg tgccgcgcat tggcgacgac gggtccgtcg
      301 cgcgggatgt cgccggcctc cagcgccgcg cgcatggccg atcgcagctc ttcgagcccg
      361 tccccggtct tcaacgagac cgccgcggcg cggcccgccc cgacgatccg cgcaaggccg
      421 tcggggctct ccgtgctcca cgccggcgcc agatcggtct tgttgatcac cacgatccgc
      481 gccgcgcccg ccgtgtcgcg cagcagcgcg cggtcggtct cgtcgagcgg ccgcgaccga
      541 tccagcacga gcagcacgag gtcggcaacc gccgcggtgc ggcgccccct cgacacggcc
      601 tcccgctcga cctcgtcttc gctcgatcga atgcccgcgg tgtccacgag cccgagccgg
      661 atgccgtcca ggtcgattgc ctcggtgagc atgtcgcgcg tggtcccggg acgcgaggag
      721 acaatggcgc ggtcggcgcc gacgagccag ttgaacagcg acgacttgcc gacgttgggc
      781 ctgccgagaa tggcgatctg ccggccttcg cggacgaggc ggccccgcgc ggccgtggcg
      841 agcagcgcgt cgatgccccc aatcacgcgc cgcgcgacgc gggccgcgtc tccggcttcg
      901 atgaagtgga acccttcgtc cgggaagtcc accgaggcct cgagccgcgc cgtcaggtcg
      961 aacagttcgc cgtcgagcgc cgcgatggcg cgcgtcaggg tgccctcgag ctggtcgaac
     1021 gcgacccgcg cctggagcgg cgtcacggcg tccaccagat cggcgaccgc ctccgcctgc
     1081 accaggtcca tccggccgtt caggaacgcc cgcagcgtga actcccccgg ctcggccagc
     1141 ctcgccccgg cgtcgatggc cgactgcagg atctcccgca gcagcgccac gcttccgtgc
     1201 gcgctgatct cgacgacgtc ctcgccggtg taggactgcg gggcgggaaa gaacgtggcg
     1261 acgacgcggt cgatcgcgcg cgcggccggg cccggcccct ctctgcccgc gggcgcctcc
     1321 cggcgccggg ccgcgcgcgt gacggtcgcg cgccgcggct cgagatcgcg cggcagcgtc
     1381 aggatcgccc gggcgcactg gcgcgcgcgc ggcccgctca gccgcacgac gccgacggcc
     1441 gcgcgcaccg gcgccgtggc aagcgccgcg atggtgtcgg cgggggagaa cacggggaca
     1501 gagtacaccg gagctcggga ttcggggctc gaggcgcggg gctcggggct cgggattcgg
     1561 gattcggact ccgcacaaca ccccgaggat ggcggagcga ggcttgatga ccggacgcgg
     1621 aggctcgaag ggctccggct gacgttgcct gcttgtctcc cccgaatccc ggatcccgga
     1681 ctgtctactt cgcgctgatg atgaccgtct tcatgaacgc gtcgccgatg ctctccgacg
     1741 tcgcgtcggc ccgttcggcg acggccaggt ggacgatccg gcgctcgtac gggttgaggg
     1801 g
//
LOCUS       DAOSIF010000588         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_56548_length_1799_cov_3.880161, whole genome shotgun sequence.
ACCESSION   DAOSIF010000588 DAOSIF010000000
VERSION     DAOSIF010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56548_length_1799_cov_3.880161"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1438
                     /locus_tag="PLT35_14145"
     CDS             164..1438
                     /locus_tag="PLT35_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG30414.1"
                     /translation="MSDVHASRWDRGSRLAAGVRRTRGGAIVLNVEAGELAALADQVS
                     LEHVLEAIAARAPDIAYVALEDGPDRLSFGPLASAAGAVDASARAPFTAVPLPSALQG
                     LAAARVSVDAAPVLEFSGPADFARPDSPVLRLGLSLDGLRRAERRTLARSMLSLAAAL
                     TLGVLAIAFVLLRRRYGALSVRHARAEEALRRRDRLAAMGELASTVAHEVRNPLNAIG
                     MTAQRLRHEFTGGGGAPAQDPNELEELLDVLSGETRRIDRIVQQFLDYARPPRLAPRR
                     ASLRDAVLEAAERQHARAATRGVTIVAEAESAGEAVFDADQLKQALDNLVRNAVDASP
                     DGGRVVLAARPHAGGHVIEVTDEGTGIPPDVLPRIFDLYFTTKADGTGVGLAVTHQIV
                     DAHRGRIDVESAPGRGTRMSVHLPADAELPNG"
     gene            1431..>1799
                     /locus_tag="PLT35_14150"
     CDS             1431..>1799
                     /locus_tag="PLT35_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG30415.1"
                     /translation="MAERVVLVVDDEAAQRTVLAGFLRKRGYEVVSAAGVDEAVQQAS
                     NRTIDLVLTDLNMPGKDGLVLIDRLRALNPELPVIVMTAYGTVASAVSAMKRGAADYL
                     TKPIDLEELDVLVARTLERRA"
BASE COUNT          238 a          665 c          662 g          234 t
ORIGIN      
        1 tggacagggc gctcgacagg gcctcggaca aggcctcggc gccggcaggc gcgggcgcgg
       61 ccccggggcc gaggccgccg ctggcggcgg gccgcggcaa gggcccggtt cagccatggc
      121 gggcctggca gagcgcctcc tccaaggggg cgagggcgag gccgtgagcg atgtccatgc
      181 gtcgcgctgg gaccgcggct cccgcctggc cgccggagtc cgccgcaccc ggggcggcgc
      241 gatcgtgctc aacgtcgaag cgggcgaact ggcggcgctg gccgatcagg tttccctcga
      301 gcacgtcctg gaggccattg cggcgcgcgc ccccgacatc gcctacgtgg ccctcgagga
      361 cggaccggat cggctgtcgt tcggcccgct ggccagcgcc gccggcgccg tcgacgcatc
      421 cgctcgagcg ccgttcacgg ccgtgcccct tccctccgcg ctgcagggac tggcggcggc
      481 cagggtgagc gtcgatgccg cgccggtcct ggagttctcg ggcccggcgg atttcgctcg
      541 ccccgacagc cccgtcctgc gactcggcct ctcgctggac ggcctccgcc gcgccgagcg
      601 gcggacgctc gcccgctcga tgctgtcgct ggcagcagcg ctgacgctcg gtgtgctggc
      661 aatcgccttc gtcctgctgc ggcggcgcta cggggcgctg agcgtcaggc acgcgcgcgc
      721 agaggaggcc ctgcggcggc gggaccgcct ggcggccatg ggggagctgg cgtccaccgt
      781 ggcgcacgag gtccgcaacc cgctcaacgc gattggcatg accgcccagc gcctccggca
      841 cgaattcacc ggagggggcg gcgcgcctgc ccaggatccg aatgagctgg aggaactgct
      901 cgacgttctt tccggcgaaa cgcggcgcat cgaccgcatc gtccagcagt tcctcgacta
      961 cgcccggccg ccgcgcctcg cgccgcggcg cgcgagcctg cgcgacgcgg tgttggaggc
     1021 cgccgagcgc cagcacgccc gcgccgccac gcgcggcgtc accatcgtcg ccgaggccga
     1081 gtcggcgggc gaggcggtgt tcgacgcgga ccagctgaag caggccctgg acaatctggt
     1141 tcgcaacgcc gtcgacgcct cgcccgacgg cgggcgcgtc gtgctcgctg cccggccgca
     1201 cgcaggcggc cacgtcatcg aggtcaccga cgaggggacg ggaattccgc ccgacgtcct
     1261 tcccaggatc ttcgacctct acttcacgac caaggccgac ggcacgggcg tcggcttggc
     1321 ggtcacccat cagatcgtgg acgcccacag ggggcgcatc gacgtggagt cggctccggg
     1381 ccggggcacg cggatgagcg tccacctgcc ggccgacgcg gagctgccga atggctgaac
     1441 gggtcgtgct ggtcgtcgac gacgaagccg cgcagcggac cgtcctcgcc ggcttcctgc
     1501 gcaagcgcgg ctacgaggtc gtcagcgccg cgggcgtgga cgaggccgtg cagcaggcct
     1561 cgaaccgcac gatcgatctc gtgctgaccg acctgaacat gcccgggaaa gacggcctgg
     1621 tcctcatcga ccgcctgcgc gccctcaacc ccgaactgcc ggtgattgtc atgacggcgt
     1681 acggcaccgt ggcgagcgcc gtgtccgcga tgaagcgtgg agccgccgac tacctcacga
     1741 agccgatcga cctcgaggag ctcgatgtcc tggtcgcgcg gacgctcgag cgccgcgcg
//
LOCUS       DAOSIF010000589         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_56645_length_1797_cov_4.770953, whole genome shotgun sequence.
ACCESSION   DAOSIF010000589 DAOSIF010000000
VERSION     DAOSIF010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56645_length_1797_cov_4.770953"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLT35_14155"
     CDS             complement(<1..255)
                     /locus_tag="PLT35_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor K domain-containing protein"
                     /protein_id="HOG30416.1"
                     /translation="MFVLAVLALAAAPAAADPLNISNISGIWINNVGGEHLKGLGTSA
                     VTWGDGVAPDSGYTFTAASDIYGAALATPLLLGTFTHHNEP"
     gene            708..>1797
                     /locus_tag="PLT35_14160"
     CDS             708..>1797
                     /locus_tag="PLT35_14160"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOG30417.1"
                     /translation="MTRHELLPTAAFFAWTLGALLPVPAAGQPPGEAQVITGVERLTW
                     TQQAFDDEELRRHAYVLYVDGAPVLLPAASCGAIPVDGQAASCESPLPELAPGFHDLE
                     LAARLASEGTVLESPRSAPLRVFVDRALPYLPSAEEEPEYQRPARRPVPAGAAVAGPR
                     RPAAATAELVVSGLDDPAALAFLPDGRLLIAERRGWLRLAAGGRLAEAPAAVLADADP
                     AGRDSWSLALAPDAARTREVFLSYAARDASGARVGRVIRFREAGGTLGEAAVVHDDLP
                     ADPVAPLARFGPDGALYVATSALDPRDAGDLGSYAGKLLRFNPDGTAAEGNPIRHSPV
                     LSSGYRARPDFDWNPADGLLWSVEPDPGG"
BASE COUNT          248 a          655 c          616 g          278 t
ORIGIN      
        1 aggctcattg tggtgcgtga aggtcccgag cagcagcggc gtggccagcg cggcgccata
       61 gatgtcgctc gccgcggtaa acgtgtatcc gctatcggga gcgaccccgt cgccccaggt
      121 cacggcggag gtgccgagcc ccttaagatg ctccccgccc acgttgttaa tccagatccc
      181 ggagatgttc gagatgttca gcggatccgc cgccgccggg gccgcagcca gcgccagcac
      241 ggcgagaacg aacattgctt ttctcattgt cctgtccttt cccaagaccg gcccttccag
      301 gctgacaccg gctgggtgta atctacgttc gcttcagcaa gggttgtgcc aatcaatgcg
      361 ccggcgaagc atgtccatgt aaagcacttg tcgataaagg cttatccgga gggtccacaa
      421 gacgaacttc ccacgatgaa ccctcagatg taaagcaggc cgacatctca ctcgatagtc
      481 cggcccatcg gccgatcggt ccctcggccg ttccctgtgg cgccgcccgg agagcctgcg
      541 gggccgaccg gatttcccgg ctgtcggaac acctggaacc gataccagga tgtggggcat
      601 cgtgaaacca tgcgaagccg taggccggcc agcctaccgc tcggtcgccc ggcccctccc
      661 atcctcttcg gagcaggtcg tgccgatatc ccatggcagg ggacgggatg acgagacacg
      721 agctgctgcc gaccgccgcc ttcttcgcct ggacgctggg ggccctgctg ccggttccgg
      781 ccgcgggcca gccgccgggc gaggcccagg tcatcaccgg cgtcgagcgg ctcacgtgga
      841 cccagcaggc gttcgacgac gaagaactcc gccggcacgc gtacgtgctc tacgttgacg
      901 gcgcgccggt tctcctgccg gcggcgtcct gcggcgccat ccctgtcgac ggccaggcgg
      961 cgtcctgcga atcgcccctg ccggagctgg cgccgggctt ccacgacctc gagcttgccg
     1021 ccaggctggc gagcgagggc accgtcctcg aaagccccag gtcggcgcct ctccgggtgt
     1081 tcgtggatcg ggcgctgcca tacctgccgt cggccgaaga ggaacccgag taccagcgcc
     1141 ctgccaggcg gcccgtgccg gccggcgctg ccgttgccgg gccgcgccgc cccgccgcgg
     1201 cgacggcgga actcgtcgtg tcggggctcg acgacccggc ggccctggcg ttcctgcccg
     1261 acggccggct tctcatcgcc gagcggcggg gctggcttcg gctggcggct ggaggccggc
     1321 ttgccgaggc tccggcggcg gtgctggccg atgccgatcc cgccggccgc gattcctgga
     1381 gcctggccct cgcccccgac gccgcgcgca cgcgggaggt gttcctcagc tacgccgccc
     1441 gagacgcctc gggcgcgcgc gtcggcaggg tgatccgctt ccgcgaggcg ggcgggacgc
     1501 tcggcgaggc cgcggtggtc cacgacgacc tgccggcgga tcctgtggct ccgctggcga
     1561 ggttcgggcc cgacggcgcg ctctacgtgg ccacgtcggc tctcgatccg cgcgacgccg
     1621 gcgacctcgg gtcgtacgcc ggcaagctcc tcaggttcaa ccccgacggc acggcggcgg
     1681 agggcaatcc aatccgccac tcgccggtgc tctcgtccgg ctaccgggcc cggccggact
     1741 tcgactggaa cccagccgac ggcctgctgt ggtccgtcga gccggaccct ggcggcg
//
LOCUS       DAOSIF010000590         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_56695_length_1796_cov_3.503733, whole genome shotgun sequence.
ACCESSION   DAOSIF010000590 DAOSIF010000000
VERSION     DAOSIF010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56695_length_1796_cov_3.503733"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLT35_14165"
     CDS             complement(<1..279)
                     /locus_tag="PLT35_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG30418.1"
                     /translation="MAPAPQFSIEDVVIGRSARMRAVFDFVRVVADSDSSVLVTGETG
                     TGKEMIANLIHESSPRRQKPFVPVSCAILTETLIESELFGHERGAFTGA"
     gene            361..585
                     /locus_tag="PLT35_14170"
     CDS             361..585
                     /locus_tag="PLT35_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOG30419.1"
                     /translation="MVTRERVQAVLDRIRPMLQSDGGDIELVDVVDNRAKVRLSGHCV
                     GCPSAQMTLYFGVESALKEEIPELEGIVVA"
     gene            complement(611..>1796)
                     /locus_tag="PLT35_14175"
     CDS             complement(611..>1796)
                     /locus_tag="PLT35_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30420.1"
                     /translation="RIFLMHNVHEDRPVVFESRWAMSYLRGPLTRDQIRALMAGRKAE
                     AAPAPPAAASAPPRPAAAAAAQPVQAAPADRPSSARPILPPDVRQFFLPVSVPPPAAA
                     SLAYAAMAVGVAAVGFGDAKLRVSHVRPLTVAARIDDRLGGVSWDEGEILALDARQLE
                     VEPRSDGVFLELPPAASRPKSYDGWAKDFRAWLAQTQTLDLLRSQAADMVSEPGETER
                     DFRVRLQTAARERRDLAVARLRKAYASKVASLDERIRRSREAVERESQQASGQRVQTA
                     VSMGATVLGALLGRKTFSTSTLGRATTAARGIGRSMKESQDVARAKEALGAAERQRAD
                     LEAQIQAEVGALGGAMDPLTEALERLVLRPKRTEVTVQLVALAWAPVWVDEAGGRTPA
                     YR"
BASE COUNT          252 a          653 c          617 g          274 t
ORIGIN      
        1 ggcgccggtg aacgcgccgc gctcgtggcc gaagagctcg gactcgatca gggtttcggt
       61 gagaatcgcg cagctcacgg gcacaaaggg cttctgccgc cgcgggctcg actcgtggat
      121 gagattggcg atcatctcct tgccggtgcc ggtctcgccg gtcacgagca cgctgctgtc
      181 gctgtcggcg acgacccgca cgaagtcgaa cacggcccgc attctcgcgc ttcggccgat
      241 gacgacgtcc tcgatgctga actgcggtgc aggagccatg gattaggggc tgccggctcg
      301 tcgcgggcgc tctaccagat gtatcgatag tatactagct tcacacgcag gagatccttg
      361 atggtgacgc gcgaacgtgt tcaggccgtg ctcgatcgca tccgccccat gctccagagc
      421 gacgggggcg atatcgaact ggtggacgtg gtggacaacc gggccaaagt ccggctctcc
      481 gggcactgcg tagggtgccc gagcgcccag atgactctct acttcggcgt ggagagcgcg
      541 ctcaaggagg agatcccgga gctcgagggc atcgtcgtcg cctgagcgag cggcggcgcc
      601 ggcgcggcgc tcaccgatag gccggcgtcc tccctccggc ttcatcgacc cagaccggcg
      661 cccaggccag cgccaccagc tgcaccgtca cctccgtccg cttcggccgc agcacgaggc
      721 gctcgagcgc ttctgtcagc gggtccatgg cgccgccgag cgcgccgact tcggcttgga
      781 tctgcgcctc gagatcggcc cgctggcgct cggcggctcc caacgcctcc ttggctctgg
      841 cgacgtcctg gctctccttc atgctccggc cgatgccccg cgcggcggtc gtcgcgcggc
      901 cgagcgtcga cgtgctgaac gtcttcctgc cgagcagggc gccgagcacg gtcgcgccca
      961 tcgagacggc ggtctggact ctctgcccgc tcgcctgctg ggactcgcgc tcgaccgcct
     1021 cccgacttcg ccggatgcgc tcgtcaagcg acgcgacctt cgacgcatag gccttccgca
     1081 gcctggccac cgccaggtcc cggcgttcgc gtgccgccgt ctggagccga acccgaaagt
     1141 cccgttcggt ttctccgggc tcggacacca tgtccgccgc ctggctgcgc agcagatcga
     1201 gcgtctgggt ctgcgccagc cacgccctga agtccttcgc ccagccgtcg tacgacttgg
     1261 gccgggacgc ggccggcgga agctcgagaa acaccccgtc gctccgcggc tccacctcca
     1321 gctgtctcgc gtcgagcgcg agaatctcgc cttcgtccca cgacacgccg ccgagcctgt
     1381 cgtcgatgcg ggccgccacg gtcagcggcc ggacgtggga caccctgagc ttcgcgtccc
     1441 cgaaaccaac ggccgcgacg ccgaccgcca tggctgcgta ggcgaggctc gccgcagccg
     1501 ggggtgggac ggacacgggc agaaagaact gcctcacgtc gggcgggaga atcggccggg
     1561 ccgagctggg cctgtccgcg ggcgcggcct gcaccggctg ggcggcagcg gcagccgccg
     1621 gacgcggcgg cgcggacgcg gcggcgggcg gtgcgggcgc ggcttccgcc ttccgccccg
     1681 ccatcagcgc gcggatctga tcgcgcgtca gcggcccgcg caggtacgac atcgcccagc
     1741 gcgactcgaa caccaccggg cggtcctcgt gcacgttgtg catgaggaag atgcgc
//
LOCUS       DAOSIF010000591         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_56793_length_1794_cov_3.264520, whole genome shotgun sequence.
ACCESSION   DAOSIF010000591 DAOSIF010000000
VERSION     DAOSIF010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56793_length_1794_cov_3.264520"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLT35_14180"
     CDS             <1..826
                     /locus_tag="PLT35_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG30421.1"
                     /translation="VGLLGPNGAGKSTLLKTLLGFLAPRSGAMTVLGFDVATSPAEIR
                     ARLGYMPEIDAHIPGMNAVSFVAYCGELAGLPAVDAVQRAHEVLYYVGLGEARYRNVE
                     TYSTGMKQRIKLAQALVHDPDLLFLDEPTNGMDPKGREEMLDLIRDLAHRKGMNLILS
                     SHLLPDVEYTCDHVVVLHQAKVAAQGPIAELKGPRGSVYEVRIKGDRDGFRSLLREAG
                     FTAHPGEEDVMRVFVPGEAGAHALFELAARRGVQVRHLRPSLPSLEDVFAQAVGEE"
     gene            830..1669
                     /locus_tag="PLT35_14185"
     CDS             830..1669
                     /locus_tag="PLT35_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG30422.1"
                     /translation="MPIHDQSYRRYAGVRTPPGRSWTVIASAGIKAMLAKRKFLGLLL
                     VAWLPFVVRAVQLYLAANFPQMSVISPSPGTFRQFLDQQGVFTFFITIFTGAGLISND
                     LRANALQIYLSKPVGRLEYVAGKFTVLAFFLLLVTWLPATLLLFVQVVFAGSVAFVAA
                     NLYLLPAILIATCVETTVVALVMLALSSLSKSARFVGVMYAGVFFFTAALHGVVQAAT
                     RESGFCWMSLRASLQNVGDLIFRVPPRSDTPAWGAALALAATVAASAWILERRVRGVE
                     VVT"
BASE COUNT          239 a          634 c          617 g          304 t
ORIGIN      
        1 cgtggggctg ctgggcccga acggagcggg caagagcacc ctcctcaaga cgctgctcgg
       61 attcctcgcg ccgcggtccg gcgcgatgac ggtgctgggc ttcgacgtgg cgacgtcgcc
      121 cgccgagatc cgcgcgcgcc tcggctacat gcccgagatc gacgcgcaca tcccggggat
      181 gaacgccgtg tcgttcgtcg cctactgcgg cgagctggcc ggcctcccgg cggtggacgc
      241 cgttcagcgc gcccacgagg tcctgtacta cgtcggcctc ggcgaggccc gctaccgcaa
      301 cgtcgagacg tactcgacgg gcatgaagca gcggatcaag ctcgcgcagg ccctggtgca
      361 cgatccggat ctcctcttcc tggacgagcc cacgaacggc atggatccga agggccgcga
      421 ggagatgctg gatctcatcc gggacctcgc gcaccgcaag ggcatgaacc tgatcctctc
      481 gtcgcacctg ctcccggacg tcgaatacac ctgcgaccac gtcgtcgtgc tgcatcaggc
      541 caaggtggcg gcgcaggggc ccatcgccga gctcaagggc ccgcgcggca gcgtctacga
      601 ggtccggatc aagggcgatc gcgacggctt caggagcctg ctccgggagg ccgggttcac
      661 ggcgcatccc ggcgaggaag acgtgatgcg ggtgttcgtg cccggagagg ccggcgcgca
      721 cgcgctcttc gagcttgccg ccaggcgcgg cgtccaggtg cggcatctgc ggccgagcct
      781 gccgagcctc gaggacgtgt tcgcccaggc cgtgggggag gagtaggccg tgcccatcca
      841 cgaccaaagc taccgccgct acgccggggt gcggacgccg ccgggccggt cgtggacggt
      901 catcgcgtcg gccggcatca aggcgatgct cgccaagcgc aagttcctcg ggctgctcct
      961 cgtggcctgg ctcccgttcg tcgtgcgcgc cgtgcagctg tacctcgcgg cgaacttccc
     1021 ccagatgtcg gtgatctcac cgtcgccggg aacgttccgc cagttcctcg atcagcaggg
     1081 cgtcttcact ttcttcatca ccatcttcac gggcgcgggc ctcatcagca acgacctgcg
     1141 ggcgaacgcg ctccagatct acttgtcgaa gccggtcggc cgccttgagt acgtggccgg
     1201 gaagttcacc gtgctggcgt tcttcctgct cctcgtcacc tggcttccgg cgaccttgct
     1261 gctgtttgtc caggtggtct tcgccggcag cgtggcgttc gtggcggcga acctctacct
     1321 gctgcctgcg atcctgatcg ccacctgcgt cgagacgacc gtcgtggcgc tcgtcatgct
     1381 cgcgctctcc tcgctgtcga agagcgcgag gttcgtgggc gtcatgtacg ccggcgtgtt
     1441 cttcttcacg gccgccctgc acggcgtcgt gcaggccgcg acccgggagt cgggcttctg
     1501 ctggatgtcg ctgcgggcga gcctccagaa cgtcggcgac ctcatcttcc gggtgccgcc
     1561 ccgctccgac acgccggcct ggggcgcggc cctcgccctg gccgccacgg tggcggcgtc
     1621 cgcctggatc ctcgagcggc gcgtgcgcgg cgtggaggtg gtcacatgac gcccctcgtc
     1681 gtggcggacc gcctgtccaa gtggtacggc caggtcatcg ggctgaacga cgtcagcgcg
     1741 tccatcccgg cggggatcac cggcctgctc gggccgaacg gcgcgggcaa gtcc
//
LOCUS       DAOSIF010000592         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_56834_length_1793_cov_3.679517, whole genome shotgun sequence.
ACCESSION   DAOSIF010000592 DAOSIF010000000
VERSION     DAOSIF010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56834_length_1793_cov_3.679517"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLT35_14190"
     CDS             complement(<1..212)
                     /locus_tag="PLT35_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30423.1"
                     /translation="MTRDYTDRPCSGRSALPALGRALAPALATEASPDDAVFVFARAE
                     QGLRMPLAVQRCRVGDLPAAFSLDDS"
     gene            291..1568
                     /locus_tag="PLT35_14195"
     CDS             291..1568
                     /locus_tag="PLT35_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30424.1"
                     /translation="MADGEVMAPGCSRPYAAAAALMALMGAFLWAIRGTAGYGGATGG
                     TLAGFGWALLWCACSRLLGLPDRRPYTGPWAMLAITAGIAFGGFTGYGVYTSWVRGAF
                     YLDYPNGLREVSAWPGYAMLFLCGFHWGGNTGCLMAWCAPETRLTRRDWMERIAWGTA
                     GALAVYGMVRLMPQAFLPFYGEGIYADQANRTCTRAVGSIRNIAPHLGLVLGFLGYEL
                     RRPDRRAAALILTMALGFAAPFAAGGWWHTRLGSSLQIDWWKNWEMTIGLVGGLAFGA
                     VFWRFNRPEPPRDEPPGSIVQALARSGLHLSLPTFVLLGGAYDGYCRLRAMAPHRAGR
                     LCLFVLTVCIPFLLRLRQRPRRGPAAACGPSLGTSLALVGLIVGAGFLVSVPPRWRLA
                     DTVLVSLYAFFVGASILLMALLRRLGRGRPASP"
     gene            1565..>1793
                     /locus_tag="PLT35_14200"
     CDS             1565..>1793
                     /locus_tag="PLT35_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOG30425.1"
                     /translation="MMTTPLTGIVPPLVTPLRDRDALDEPGLERLIEHVLGGGVSGLF
                     ILGTTGEGPSLSYRLRRELVARTCRQVNGRVP"
BASE COUNT          215 a          646 c          639 g          293 t
ORIGIN      
        1 atcgagtcgt ctagcgagaa cgccgcggga agatcaccca ccctgcagcg ctgcaccgcc
       61 agcggcatgc gcagcccctg ctcggcacgc gcgaagacga agaccgcatc gtcgggcgaa
      121 gcctcggtgg cgagcgccgg ggccagcgcg cgtcccagcg ccggaagcgc cgaccgtcct
      181 gagcacggcc tgtctgtata atcccgggtc atcaggcgcc gagcggcgcg atcggacgcc
      241 gacagacccg ggaggcatac aggaagccgc agcccgcgga gggaggcgtg atggcggacg
      301 gagaagtgat ggcgcctggc tgcagccggc cgtacgccgc tgcagccgcc ctgatggccc
      361 tgatgggcgc gttcctgtgg gccatccggg ggacggcggg ctacggcggc gcgacaggcg
      421 gcacgctggc cggctttgga tgggcgctgc tctggtgcgc gtgttcccgc ctgctcggcc
      481 tgcccgatcg ccggccctac accggcccct gggccatgct cgccatcacc gccggcatcg
      541 ccttcggcgg attcacgggt tacggcgtct atacgtcctg ggtgcgcggc gcgttctacc
      601 tggactatcc gaacggcttg cgcgaggtct ccgcctggcc gggctacgcg atgctcttcc
      661 tctgcggctt ccactggggc ggcaacaccg gctgcctgat ggcctggtgc gctcccgaaa
      721 cgcgcctgac gcgccgcgac tggatggagc ggatcgcctg gggaaccgcg ggtgccctgg
      781 ccgtgtacgg gatggtccgg ctgatgccgc aggcgttcct ccctttctac ggtgagggca
      841 tctacgccga tcaggcgaac cggacctgca cgcgtgccgt cggctcgata cggaacatcg
      901 ccccgcacct gggcctggtc ctgggcttcc tcggctacga gctccgccgc cccgaccggc
      961 gcgccgccgc gctcatcctg acgatggcac tcgggttcgc ggcgccgttt gccgcagggg
     1021 gatggtggca caccaggctg ggctcgagcc tgcagatcga ctggtggaag aactgggaga
     1081 tgacgatcgg gctcgtcgga ggcctcgcgt tcggtgccgt cttctggcgc ttcaatcgcc
     1141 cggagccgcc acgcgacgag ccgccaggct ccatcgtgca ggcgctcgcc cgctccggac
     1201 ttcacctctc gcttcccacg ttcgttctcc tcggcggcgc ctacgacggg tattgccgcc
     1261 tgagggcgat ggccccccac cgggcggggc ggctgtgcct cttcgtgctg accgtctgca
     1321 tccctttcct gctccggctg cgccagcgcc cgcggcgcgg gcccgccgcc gcgtgcgggc
     1381 cttcgctcgg gacgtcgctg gcgctcgtcg gactgattgt cggcgcgggg ttcctggtca
     1441 gcgtgccgcc gcgatggcgg ctggcggaca ccgtgctcgt ctcgctctat gccttcttcg
     1501 tgggcgcgag tatcctgctc atggccttgc tccggcgtct gggccgcggg aggccggctt
     1561 cgccatgatg acgacaccgc tcaccggcat cgtgcctccg ctcgttaccc cgctccgcga
     1621 tcgggacgcg ctcgacgagc cggggctcga gcgcctgatc gagcacgtcc tcggcggagg
     1681 cgtgagcggg ctgttcatcc tcggcacgac gggcgagggg cccagcctga gctatcggct
     1741 gcgccgcgag ctcgtcgcgc gcacgtgccg gcaggtcaac ggccgcgtgc cgg
//
LOCUS       DAOSIF010000593         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_57321_length_1782_cov_4.125651, whole genome shotgun sequence.
ACCESSION   DAOSIF010000593 DAOSIF010000000
VERSION     DAOSIF010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57321_length_1782_cov_4.125651"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLT35_14205"
     CDS             <1..637
                     /locus_tag="PLT35_14205"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30426.1"
                     /translation="APRIAASAAVMAAGRLAARPPCCKPAVQEPAMTPAPSRRRPVVG
                     VMGSSTSPYEDLAAPLGRWLASLGVHLLTGAGAGTMAAVSRAFVETQPRAGQAIGIVP
                     ADLVAGVAAPRPGYPNPFVEIAVFTHLPLSGIAGTDPRSRNHINILSSDVVIVLPGGE
                     GTRSEAELAVRYGKPAVAHFGDNPVDWSPPEGVPLARTLADVQAFVRAALS"
     gene            complement(782..>1782)
                     /locus_tag="PLT35_14210"
     CDS             complement(782..>1782)
                     /locus_tag="PLT35_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOG30427.1"
                     /translation="FGSKVSRYNVVDATPFRRDIVKELAEACAKRGMRLGFYYSQAQD
                     WHEPNGAGNDWDFKPNAEKDFDQYLRDKAYPQVRELLTGYGPVALVWFDTPQLMTSER
                     ARRFTELVRSLQPDTLIDGRLGAEGDYISTGDNIIPGEKQTEAWETPATINHTWGYRK
                     DDVDWKNPGEIVFKLVDIVSKGGNYLLNVGPMADGVIPQASQDVLRAAGQWLRINGEA
                     VYGAWPTPFGEEFGEASAKGAKDLRGSPLFLSWNDYRVTAKPGKLYFTIFIEPRVPVE
                     LPPMKNAVKRAYFLANGLPVAIAEKDGRQSFTVPRPILDPMGTVIVVEIEGERVAR"
BASE COUNT          231 a          662 c          583 g          306 t
ORIGIN      
        1 cgccccgcgc atcgcggctt cggccgccgt catggccgcg ggccgcctcg ccgcgcggcc
       61 gccctgctgc aaacccgccg ttcaggagcc agccatgacg cccgccccgt ccaggcgccg
      121 tcccgttgtc ggcgtgatgg gatccagcac cagcccgtac gaggatctcg cggcgccgct
      181 cggccgatgg ctggcctcgc tcggcgtgca cctgctcacg ggcgccggcg ccggcacgat
      241 ggcggcggtc agccgcgcct tcgtcgaaac gcagccccgc gcgggtcagg cgatcggaat
      301 cgtgccggcc gacctcgtcg cgggcgtcgc agcgccgcgg cccgggtacc cgaacccgtt
      361 cgtcgaaatc gcggtcttca cgcacctgcc gctcagcggc atcgccggca cagatccccg
      421 cagccgcaat cacatcaaca tcctgtcctc ggacgtggtg atcgtgctcc ctggaggcga
      481 gggcacgcgc tcggaagccg agctcgccgt ccgctacggc aagccggcgg tcgcgcactt
      541 cggcgacaac ccggtggact ggagcccgcc cgagggcgta ccgctcgcgc gcacgctggc
      601 cgacgtgcag gcgttcgtcc gcgccgccct ttcgtagaac ccgggaaacc ggggtcagat
      661 ccaggattcc ggtgccaggt cggggttcgg gccacccctc cccggtcggg gttcgggggt
      721 ctggggccgg ggattctgag ttccggattc cgacttcggc gtcccccgcc cctcctgtgc
      781 cctaccgcgc gacccgttcg ccttcaattt cgaccacgat gaccgtgccc atcgggtcca
      841 ggatcggcct cggcaccgtg aacgattggc ggccgtcctt ctccgcgatc gccaccggca
      901 gcccgttggc caggaagtag gcccgtttca cggcgttctt catgggcggc agctcgactg
      961 gcacgcgcgg ctcgatgaag atcgtgaagt acagcttccc tggcttggcc gtcacccggt
     1021 agtcgttcca ggacaggaac agggggctgc cgcggaggtc cttcgcaccc ttcgcgctgg
     1081 cctcgccgaa ctcctcgccg aacggcgtcg gccacgcgcc gtagacggcc tcgccgttga
     1141 tcctcagcca ctgtccggcc gcgcggagga cgtcctggct cgcctgcggg atgacgccgt
     1201 ccgccatggg cccgacgttc agcaggtagt tgccgccctt gctgacgatg tccaccagct
     1261 tgaagacgat ctctcccggg ttcttccagt ccacgtcgtc tttgcggtag ccccacgtgt
     1321 ggttgatcgt cgccggcgtc tcccacgcct ccgtctgctt ctcgcccggg atgatgttgt
     1381 cgccggtgct gatgtagtcg ccctccgccc cgagccgccc gtcgatgagc gtgtccggct
     1441 gcaacgatct cacgagctcc gtgaaccgcc gcgcgcgctc ggaggtcatc agctggggcg
     1501 tgtcgaacca gacgagggcc accggcccgt agccggtgag gagctcgcgc acctggggat
     1561 aggccttgtc gcgcaggtac tgatcgaagt ccttctccgc gttcggcttg aagtcccagt
     1621 cgttgccggc gccgttgggc tcgtgccagt cctgcgcctg cgagtagtag aacccgagcc
     1681 gcatgccgcg tttcgcgcac gcctcggcca gctctttcac gatgtcgcgc cggaacggcg
     1741 tcgcgtccac gacgttgtag cggctgacct tcgacccgaa ca
//
LOCUS       DAOSIF010000594         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_57491_length_1779_cov_2.788863, whole genome shotgun sequence.
ACCESSION   DAOSIF010000594 DAOSIF010000000
VERSION     DAOSIF010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57491_length_1779_cov_2.788863"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..>1779)
                     /locus_tag="PLT35_14215"
     CDS             complement(174..>1779)
                     /locus_tag="PLT35_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOG30428.1"
                     /translation="IVEVDIPYRDWINPETGKSEGFEDYSPYYTPQFAMFYGLVGSTM
                     ETPYKSEDGVDAHYYGILEGAKYTAEHRVGMLTNQIKRFQRADRGKSQPVTADVPEPM
                     EYGFGYIIPSGGPLQKNATAARKAVRHLLANGIEVYRTTEPVRPVLRKRISDFPVGSY
                     FVPLRQPLRGIANAMLWYGQDVSSQATQMYDACAWQLPESWGFSRYELPRPVSFGRKP
                     VTKVAEPAGQFVGAGPWYTVEADGDVDVKVVNQLVAHGIPVERVTWTAGGESDPPLGA
                     FLIFAASRTAKDFLAAKAAANHCRISTVQYDAGNFGTQHVYHEGYPPGSVAGPLKVAV
                     YSDGPTAFALRKLGFDAQVVSADVDLGRYDTLVCDDYTGLDVDGLKAWIYDGGAYVAN
                     GPYGILDDVLDVQPAGGYDWSFYWANNCLGETSYHETLATAGIGGDGYTFAFPITWFD
                     DPSGGVAVDATYRDPYFLAGFWQTDDPTGLVIGDAAGKAVVVHGPYGDGRVTFIGPMA
                     AFRAHTEGTYRLLANAIYTGNWRDVE"
BASE COUNT          263 a          604 c          611 g          301 t
ORIGIN      
        1 ggccgtcccg ccgcagccgc cggacctccc cgccgccgac gggctcgtgg actgacgtgg
       61 ccccggccgt cgcccgggga cgtggggcgt cccggccgcg tctctcacgc gccctctggc
      121 gcagcaccgg gacgggcggc gcgccgcccg tcccggtgcg ttgcgcgcct acctcactcg
      181 acgtcgcgcc agttgcccgt gtagatcgcg ttggcgagca gccggtacgt gccctctgtg
      241 tgcgcgcgga aagccgccat cgggccgatg aaggtcacgc gtccgtcgcc gtacggaccg
      301 tgcaccacca cggccttgcc ggcggcatca ccgatcacga ggccggtcgg gtcgtcggtc
      361 tgccagaagc ccgccaggaa gtaaggatcg cggtaagtgg cgtcgacggc cacgcccccg
      421 ctcgggtcgt cgaaccaggt gatggggaag gcgaaggtgt agccgtcgcc tccgatgccc
      481 gccgtcgcca gagtctcgtg atacgaggtc tctccgagac agttgttcgc ccagtagaac
      541 gaccagtcgt agccgcccgc gggctgcacg tcgagcacat cgtcgaggat gccgtagggg
      601 ccgttggcga cgtaggctcc gccgtcgtag atccacgcct tgaggccgtc gacgtccagt
      661 cccgtgtagt cgtcgcacac cagcgtgtcg tacctgccga ggtcgacgtc cgcgctgacg
      721 acttgggcgt cgaatccgag cttgcgcaga gcgaaggccg tggggccgtc ggaatacacg
      781 gccaccttga gcggaccggc gacgctcccc ggcgggtagc cctcatggta cacgtgctgg
      841 gtgccgaagt tcccggcgtc gtactgcaca gtgctgatgc ggcagtggtt cgccgccgcc
      901 ttcgcggcga ggaagtcctt cgccgtcctg ctcgcggcga agatgaggaa cgctccgagc
      961 ggcggatcgc tctcgccccc ggccgtccac gtgacgcgct ccacggggat gccgtgcgcc
     1021 accagctggt tgacgacctt gacgtcgacg tctccatcgg cctccacggt ataccatggt
     1081 ccggcgccga cgaactgacc ggccggctcg gcgaccttgg tcaccggctt cctgccgaac
     1141 gacaccggtc gaggcagctc atagcgcgag aatccccagc tctcggggag ctgccaggcg
     1201 cacgcgtcgt acatctgcgt ggcctgggaa gagacgtcct ggccgtacca cagcatggcg
     1261 ttcgcgatac cacgcagggg ttgccgcaac ggcacgaagt acgaaccgac ggggaagtcc
     1321 gagatccgct tgcggaggac cggacggacc ggctcggtgg tgcggtagac ctcgatgccg
     1381 ttggcgagca ggtgccgcac cgccttacgc gctgcggtcg cgttcttctg cagcgggccg
     1441 ccggacggga tgatgtagcc gaagccgtac tccatcggct cgggtacgtc cgccgtcacc
     1501 ggttgtgact tgcccctgtc ggcacgctga aagcgcttga tctggttggt cagcatgccg
     1561 acgcggtgct cagccgtata cttggcgcct tcgaggatgc cgtagtagtg ggcgtccacg
     1621 ccgtcttcgc tcttgtacgg ggtctccatg gtggagccga cgaggccgta gaacatggcg
     1681 aactgcggcg tgtaatacgg gctgtaatcc tcgaagcctt cgctcttgcc cgtctcgggg
     1741 ttgatccagt cgcggtacgg gatgtccacc tcgacgatg
//
LOCUS       DAOSIF010000595         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58092_length_1765_cov_3.617544, whole genome shotgun sequence.
ACCESSION   DAOSIF010000595 DAOSIF010000000
VERSION     DAOSIF010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58092_length_1765_cov_3.617544"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLT35_14220"
     CDS             <1..402
                     /locus_tag="PLT35_14220"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOG30429.1"
                     /translation="GGEMLGKPADGRDARRMLERLSGRTHDVLTGVAVVCGADAELAV
                     ESTAVSFRELSASEVDWYVATGEPADKAGAYAVQGLGSRFVARVTGSYSNVVGLPLET
                     VQRLLEAAGVVRAWRPEAAAGGGREAEGALD"
     gene            462..878
                     /locus_tag="PLT35_14225"
     CDS             462..878
                     /locus_tag="PLT35_14225"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOG30430.1"
                     /translation="MKRKALKVGLTVLMLVLALGGLLYSALSEGTEYYAHVDEVLADP
                     GRWHGKDLQLHGFVVKDSVLRKRDSLEYRFKVQSRGAVLEATYDGIVPDTFTDECEVV
                     LKGRLRDGEFHVAPNGVMAKCPSKYEAKRGLARPGA"
     gene            1003..>1765
                     /gene="ccsA"
                     /locus_tag="PLT35_14230"
     CDS             1003..>1765
                     /gene="ccsA"
                     /locus_tag="PLT35_14230"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOG30431.1"
                     /translation="MASLGSFLLMAAFVAAACAVAASLSGARRGSRRLVESAVGAFYL
                     TAALMTAASAVIVRAFLTDDYAIKYVHRYSDHLQPLFYKLTSYWGGLDGSIMFWVFLL
                     AVFGSLAVRVNRDRHRELVPYVVAIVSAVEMFFLLMMLAHSNPFDTYLVQSPDDGQGL
                     NPLLQNMYMVVHPPSLYIGFVGMTIPYAFGMAALITGRLDDAWLRAVRRWTMVSWLFL
                     TLGLVLGMIWAYEELGWGGYWAWDPVENAGLLPWFT"
BASE COUNT          238 a          601 c          621 g          305 t
ORIGIN      
        1 ggcggggaga tgctcggcaa gccagccgac gggcgtgacg cacggcggat gctcgagcgg
       61 ctctcgggcc ggacgcacga cgtgctgacg ggggtggcgg tcgtctgcgg ggcggatgcc
      121 gagctggcgg tggagtccac ggcggtcagc tttcgcgagc tgagcgcctc ggaagtagac
      181 tggtacgtcg ccactggaga accggccgac aaggccggcg cgtacgccgt ccagggcctg
      241 ggttcgcgct tcgtcgcgcg tgtgacaggg tcctacagca acgtcgtcgg tctgccgctc
      301 gaaaccgtcc agcggttgct cgaggcggct ggcgtcgtgc gcgcgtggcg gcccgaggcg
      361 gccgccggcg gggggcgcga agccgagggg gcactcgatt gaccgccccg gcccccgagg
      421 gtatcctgaa aaagcggttc tcttcagcca cttgacttgc catgaaacgc aaagccctca
      481 aggtcggcct caccgtcctg atgctcgttc tcgccctcgg gggactcctg tactcggccc
      541 tcagcgaggg caccgagtac tacgcccatg tcgacgaggt actggccgac cccggtcgct
      601 ggcacgggaa ggacctccag ctccacggct tcgtggtcaa ggactcggtg ctccggaagc
      661 gggactcgct cgagtaccgc ttcaaggttc agagccgggg agcggtcctc gaggcgacct
      721 acgacggcat cgtgccggac acgttcacag acgagtgcga ggtcgtcttg aagggccggc
      781 tccgcgacgg cgagttccac gtcgccccga acggcgtcat ggcgaagtgc ccctcgaagt
      841 acgaagccaa acgggggctc gccaggcctg gcgcctgacg gagggccggt gcgggcccgg
      901 gtgatccgcg ttccacgttc cgtccaccat ccatcctgcc tgccggcgac gcgccagcgc
      961 gcgggttcgc ccccggccgc tgccggcggg cgcgggtgac acatggcctc tctgggttcg
     1021 ttcctgttga tggcggcctt cgtcgcggcg gcctgcgcgg tcgccgcctc gctctcgggc
     1081 gcgcggcggg gctcgcgccg gctcgtcgaa agcgcggtcg gcgccttcta cctgacggcg
     1141 gccctcatga cggccgcctc ggcggtgatc gtccgggcgt tcctcaccga tgactacgcg
     1201 atcaagtacg tccaccgcta ctcggaccac ctccagccgc tcttctacaa gctgacctcg
     1261 tactggggcg gcctggacgg ctccatcatg ttctgggtgt tcctgctggc ggtgttcggc
     1321 tcgctggcgg tgcgcgtcaa ccgcgatcgg caccgcgaac tcgtcccgta cgtcgtcgcc
     1381 atcgtctcgg ccgtggagat gttcttcctc ctgatgatgc tggcccacag caacccgttc
     1441 gacacctacc tcgtccagtc gcccgatgac ggccagggcc tgaatccgct cctccagaac
     1501 atgtacatgg tggtgcatcc gccgagcctc tacatcggct tcgtcggcat gaccattccg
     1561 tacgcgttcg ggatggcggc cctcatcacg ggccgcctgg acgacgcgtg gctgcgcgcc
     1621 gtgcgccgct ggaccatggt gagctggctg ttcctgacgc tcggcctcgt gctcggcatg
     1681 atctgggcgt atgaggagct ggggtggggc ggctactggg cctgggaccc ggtcgagaac
     1741 gcgggcctgc tgccgtggtt cacgg
//
LOCUS       DAOSIF010000596         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58146_length_1764_cov_3.381510, whole genome shotgun sequence.
ACCESSION   DAOSIF010000596 DAOSIF010000000
VERSION     DAOSIF010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58146_length_1764_cov_3.381510"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..950)
                     /locus_tag="PLT35_14235"
     CDS             complement(216..950)
                     /locus_tag="PLT35_14235"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG30432.1"
                     /translation="MAPAHPIVAMFEARGGAGRAAGPSLSVGLLAADLMALGGGVARL
                     ERAGVRLAHFDVMDGCYVPTLTVGPPFVKAVRTSMLKDVHLMIREPLPSLREYVAAGA
                     DLVTIHPDACAHPHRALQWLGEARTARDPERAVARGVALNPGVPLGVLEPLIDEVELV
                     TLVAINPGWGGQACVPSIFRRIDAVRERIAASGRAILLAVDGGITRDNVGALAGRGVA
                     IVVTGSAVFDGDVEANLAAMTAALRG"
     gene            complement(954..>1764)
                     /gene="lsrF"
                     /locus_tag="PLT35_14240"
     CDS             complement(954..>1764)
                     /gene="lsrF"
                     /locus_tag="PLT35_14240"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HOG30433.1"
                     /translation="HQLDWGMRNRLSRVFRPSTGRTVMLAIDHGYFLGPTTGLERVDL
                     SIVPILPHADALMCTRGILRSSIPSTCGAGVVVRASGGPSTLKELSNERLALDIDDAL
                     RMNASAMAVQVYIGGEHETQSVKNMTTLVDLGLRYGVPTMGVTAVGKQLTRDARYLGL
                     ATRICAELGAQVVKTYYCDRGFEQVTAGCPVPIVMAGGKKLPELEALTMAHHAVSQGA
                     AGVDMGRNIFQSAAPAAMIQAVGKVVHELMPPKQAYELYRTLAHEHAAAAR"
BASE COUNT          237 a          668 c          621 g          238 t
ORIGIN      
        1 cggctgcctt cggggtgtat tccggagcaa acggaacatc gcttgtccag ggatcgttcc
       61 agcggttgat catcgcccgc gccccttcga acgcacccgc ctccggattg ggcgcgccgg
      121 gtccgacgcc cggcttccgc gcccgcatgg gacccggggc cgccgggccc cgggaacgca
      181 cgcgcggcgg cggccctctc ccgcttccag gcggatcacc cgcgcagggc ggccgtcatc
      241 gccgcgaggt tcgcctccac gtcgccgtcg aacaccgcgc tcccggtcac cacgatggcg
      301 acgccgcggc cggcgagcgc gcccacgttg tcccgggtga tgccgccgtc cacggccagg
      361 aggatcgcgc ggccgctggc cgcgatccgc tcgcgcacgg cgtcaatccg cctgaagatc
      421 gacgggacac acgcctgccc tccccatccc gggttgatgg ccacgagcgt cacgagctcg
      481 acctcgtcga tgagcggctc gagcacgccg agcgggacgc ctggattcag cgccacgcca
      541 cgggcgaccg cgcgctcggg gtcccgggcc gttcgggcct cgcccagcca ctgcagggcg
      601 cggtgggggt gcgcgcaggc gtccgggtgg atcgtgacca ggtcggcgcc cgccgcgacg
      661 tactcgcgga gcgagggcag cggctcgcgg atcatcaggt gcacgtcctt gagcatcgac
      721 gtccgcaccg ccttgacgaa gggcgggccg acggtcagcg tcggcacgta gcacccgtcc
      781 atgacgtcga aatgcgcgag gcgcacgccg gcccgctcga gtcgcgccac gccgccgccg
      841 agcgccatca ggtcggccgc cagcaggccg acgctcagcg acggcccggc cgcccgtccg
      901 gcgccgccgc gcgcctcgaa catcgcgaca atcgggtgcg cgggcgccat ggcctaccgc
      961 gccgccgcgg cgtgctcgtg cgccagcgtc cgatacagct cgtacgcctg cttgggcggc
     1021 atcagctcgt gcaccacctt gccaaccgcc tggatcatcg cggcgggggc ggcagactgg
     1081 aagatgttgc ggcccatgtc cacgccggcc gcgccctggc tgaccgcgtg gtgcgccatg
     1141 gtcagggctt cgagctcggg cagcttcttg ccgcccgcca tcacgatcgg caccgggcag
     1201 ccggccgtca cctgctcgaa cccgcggtcg cagtagtagg tcttcacgac ctgcgcgccc
     1261 agctcggcgc agatgcgcgt cgcgagcccg aggtagcgcg cgtcgcgcgt cagctgcttc
     1321 cccaccgccg tcacgcccat ggtcggcacg ccgtagcgca gcccgaggtc cacgagcgtc
     1381 gtcatgttct tcaccgactg cgtctcgtgc tcgccgccga tgtagacctg cacggccatc
     1441 gcggacgcgt tcatccgcag cgcgtcgtcg atgtcgaggg cgaggcgctc gttcgacagc
     1501 tccttgagcg tgctcgggcc gccgctcgcg cgcacgacca cgccggcgcc gcacgtcgag
     1561 gggatgctcg agcgcaggat gccgcgcgtg cacatgagcg cgtcggcgtg cggcaggatc
     1621 ggcacaatcg acaggtccac gcgctcgagc cccgtggtcg gcccgaggaa gtagccgtga
     1681 tcgatcgcca gcatgaccgt gcggcccgtc gaggggcgga agacgcggga caagcggttg
     1741 cgcatgcccc agtcgagctg gtgg
//
LOCUS       DAOSIF010000597         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58196_length_1763_cov_3.244731, whole genome shotgun sequence.
ACCESSION   DAOSIF010000597 DAOSIF010000000
VERSION     DAOSIF010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58196_length_1763_cov_3.244731"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1241
                     /locus_tag="PLT35_14245"
     CDS             114..1241
                     /locus_tag="PLT35_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG30434.1"
                     /translation="MRIAIDVRKLHDFGIGTYVRNLLKYLALLDQDTEYFPLCRPQDA
                     EALAALGPNFKPVPVASKPYSVSEQIVLPARLVPRRVDLLHEPHYVLPPLVPCRAVVT
                     VHDCIHLMFPQYLPNRMAYAYARGSLWAATRRAERIFTVSETSKADILRYCDVPADRI
                     VVVYNAIDDRFSVAPSDEAIRRARERYQLDGPFALYVGNIKPHKNIERLVDAFALVRR
                     DGFERIELLIIGDRISKFPRLRRAVDRHKLHKHVRFLGFVPDDTLAALYRLATLFVFP
                     SLYEGFGLPPLEAMASGTPVVTSNRSSLPEVVGDAAVLVDPYSAPSIAEGIRRVLADA
                     SLRRALSERGLARARQFSWEASVRRVHDVYMDVASGRPPAS"
     gene            1238..>1763
                     /locus_tag="PLT35_14250"
     CDS             1238..>1763
                     /locus_tag="PLT35_14250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG30435.1"
                     /translation="MRIAFVHDWLTGMRGGEKVLEALVSLWPDADVFTLVHARGSVSP
                     AIEARRIRTSAIQRLPAATRVYRHYLPLFPAAIEMLDLDAYDLVISSSHCAAKAAVVP
                     GRARHVCYCHSPMRYAWDQFAEYFGAERVGRAAEAAYRPVMAWLARWDAGTAPRVHRF
                     LANSAFVASRIRRFY"
BASE COUNT          241 a          643 c          585 g          294 t
ORIGIN      
        1 cacgtcgagc cgcgcggcga gcgtcctgcg cctgccgagc gcgcggcgcc gccggcgcgg
       61 cctcgtcgga ccgcgggcct gtggtatcgt gaacccgcgg caggggtggc tgggtgcgca
      121 tcgcgatcga cgtccgaaaa ctgcacgact tcgggatcgg gacctacgtc cggaacctgt
      181 tgaagtacct cgccctgctg gatcaggaca ccgagtactt cccgctctgc cggccgcagg
      241 acgcagaggc cctcgcggcc ctcggcccga acttcaagcc ggttcccgtg gcgtccaaac
      301 cctactcggt gtcggagcag atcgtgctgc ccgcgcgact cgtgccgcgg cgcgtcgatc
      361 tgctccacga gcctcactac gtgctgccgc ccctggtgcc ctgccgcgcg gtggtgacgg
      421 ttcatgactg cattcacctg atgttcccgc agtacctgcc caaccggatg gcctacgcgt
      481 acgcgcgggg gagtctctgg gccgcgacac gccgcgccga gcggatcttc accgtctcgg
      541 agacctcgaa ggccgacatc ctgcgctact gcgacgtgcc cgcggaccgc atcgttgtcg
      601 tgtacaacgc catcgacgac cggttctcgg tggcgccgtc cgacgaagcg atccggcggg
      661 cccgggaacg ctatcaactc gacggcccgt tcgcgctcta cgtgggcaac atcaagccgc
      721 acaagaacat cgagcgcctg gtcgacgcct tcgctctcgt gcgccgggac gggttcgagc
      781 ggatcgagct gctcatcatc ggggaccgga tttcgaagtt tcccaggctg cgccgcgccg
      841 tagacagaca caagctgcac aaacacgtcc gcttcctcgg cttcgtgccc gacgacacgc
      901 tggccgccct ctaccggctg gcgaccctgt tcgtcttccc gtcgctctac gaaggattcg
      961 ggctcccgcc gctcgaggcg atggccagcg gaacgccggt ggtcacctcg aaccggtcgt
     1021 ccctgcccga agtggtcggc gacgcggcgg tgctcgtcga tccgtacagc gcgccatcga
     1081 tcgcagaggg catccgccgg gtgctggccg acgcttcgct gcgccgggcg ctgtcggagc
     1141 ggggcctggc ccgggcccgc caattctcct gggaggcgtc ggtccggcgg gtccacgacg
     1201 tctacatgga cgtggcttcg ggccggcctc ctgcgtcatg agaatcgcct tcgtccacga
     1261 ctggctcacg ggcatgcgcg gcggcgagaa ggtcctcgaa gccctcgtgt cgctctggcc
     1321 cgatgccgac gtgttcaccc tcgtgcatgc gcgggggtcg gtgtcgccgg cgatcgaggc
     1381 ccgccgcatt cgcacgtcgg ccatccagcg cctgccggcc gccacgcggg tttaccgcca
     1441 ctacctgccg ctgtttcccg cggccatcga gatgctcgac ctcgatgcct acgatctggt
     1501 gatcagctcg agccactgcg cggcaaaggc ggcagtcgtc cccgggcggg cacgccacgt
     1561 ctgctactgc cactcgccca tgcggtacgc ctgggaccag tttgccgagt acttcggcgc
     1621 cgaacgggtc ggccgagccg ccgaggcggc gtaccggccc gtcatggcgt ggctggcgag
     1681 atgggacgcc ggcacggcgc cgcgcgtcca ccggttcctg gcgaactccg cgttcgtggc
     1741 ctcgcgaatc cgccgcttct acg
//
LOCUS       DAOSIF010000598         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58274_length_1761_cov_2.701641, whole genome shotgun sequence.
ACCESSION   DAOSIF010000598 DAOSIF010000000
VERSION     DAOSIF010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58274_length_1761_cov_2.701641"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLT35_14255"
     CDS             complement(<1..800)
                     /locus_tag="PLT35_14255"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOG30436.1"
                     /translation="MALDQAQVLRALASVKDPDLHKDIVSLGFVKDVAIDGGRVSCTI
                     ELTTPACPVKDQMKEQARAAVMALPGVSSVDVTMTARVRTAASPDADRTHVPGVKNVI
                     AVGAGKGGVGKTTVAVNLALALAAAGSRVGLMDADIYGPNVPIMLGLSGPLASDDGKI
                     VPPEKHGLRVVSIGFLTSDESPLIWRGPMLHSVIRQFLLDVHWGELDYLVVDLPPGTG
                     DVALSLSQTAHIAGAIAVTSPQRVAVADTRRAVVMYQNNLRQGSVKTKT"
     gene            complement(883..1149)
                     /locus_tag="PLT35_14260"
     CDS             complement(883..1149)
                     /locus_tag="PLT35_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30437.1"
                     /translation="MSDSGEGLVDASTRLAERMDEREEERRQARSGARRLDPARAEAI
                     ESLRLARADLRRQTESASHDARRRQLRLALDEIERRLAALDHPA"
     gene            1332..>1761
                     /locus_tag="PLT35_14265"
     CDS             1332..>1761
                     /locus_tag="PLT35_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30438.1"
                     /translation="MSDRRRTALNTAVASLTLAAGLGLVGWFDYSSTRREMLTLLVDQ
                     AASLRQAIAVAASAGEAASAQARTALTARLLDNARLLDRLDLRGGLRQSDLDQISESN
                     RLFRVTVFSSSGQPDLTSGRSGPLFGAGRGLGPGLGQGARQ"
BASE COUNT          239 a          610 c          635 g          277 t
ORIGIN      
        1 gtcttggtct tcaccgaccc ttgccggaga ttgttctggt acatgaccac cgcgcgcctc
       61 gtgtcggcaa ccgcgacacg ctggggcgac gtcacggcta tggcgccggc gatgtgcgcc
      121 gtctggctga ggctgagggc cacgtcgccg gtgccgggag ggagatccac cacgaggtag
      181 tcgagctcgc cccagtgcac gtccagcagg aactgccgga tgacgctgtg cagcatcggc
      241 ccgcgccaga tgagcggcga ctcgtccgac gtcaggaagc caatcgaaac gaccctcagg
      301 ccgtgcttct ccgggggcac gatcttgccg tcgtccgagg cgaggggtcc gctgagcccc
      361 agcatgatcg gcacgttcgg gccgtagatg tcggcgtcca tgaggcccac gcggctgccg
      421 gccgcggcga gggcgagcgc gaggttcacg gcgacggtcg tcttgccgac gccgcccttg
      481 ccggcgccga ccgcgatgac gttcttcacg cccggcacgt gcgtcctgtc cgcgtcgggt
      541 gaggcggccg tccggacccg cgcggtcatc gtgacgtcca cgctcgacac gccgggcagg
      601 gccatgacgg ccgcgcgcgc ctgctccttc atctggtcct tgacggggca ggcgggcgtc
      661 gtcagttcga tggtgcacga cacgcggccg ccgtcaatgg cgacgtcctt gacgaagccc
      721 agggagacga tgtccttgtg gaggtccggg tccttgaccg acgcgagcgc ccggagcacc
      781 tgtgcttgat cgagagccat gttcgtagct tatgtgaaaa ggagggccgc cacaaatgca
      841 gcccggggag cgccgcccgc gcgcgctgcg ccgcgccggc gatcaggccg gatgatcgag
      901 agcagccagc cggcgctcga tttcgtcgag ggcgaggcgg agttggcggc ggcgggcgtc
      961 gtgcgacgcc gactccgtct ggcggcggag atcggccctg gcgaggcgga gcgactcgat
     1021 ggcctcggcc ctggcgggat ccagccgcct cgcgccgctc ctcgcctgcc gccgctcttc
     1081 ttcgcgctcg tccatccgtt cggcgagccg cgtgctggcg tcgacgaggc cttcaccgct
     1141 gtccgacatg agtcttctgc cgccctccga ttcgtgtggg ggtgtccctc ttctgattat
     1201 atcggctcgg ctccctgccg cgacatcccg ccggccgcgg tccgctgcgc tatagtggcg
     1261 cggagggcga gtccatgcac ccccgggagg cggtaggccg tctctcgagg ggctcaggag
     1321 gaacgcaccg gatgtcagac cgacgccgca cggcgctgaa caccgcggtc gcgagcctga
     1381 cgctggcggc gggcctcggg ctggttggct ggttcgacta ctccagcacg cggcgcgaaa
     1441 tgctcacgct gctcgtcgat caggccgctt cgctccgcca ggcgatcgcg gtggcggcca
     1501 gcgccgggga agcggcgagc gcccaggccc gcacggcgct cacggcccgc ctgctcgaca
     1561 acgccaggct cctcgacagg ctcgatctgc gcggcgggct gaggcagtcg gacctcgacc
     1621 agatctcgga gagcaaccgc ctgttccgcg tcacggtgtt ttcctcctca gggcagcctg
     1681 acctgacgag cggcaggagc gggcctctgt tcggcgccgg gcgaggcctg ggaccagggc
     1741 ttggacaggg cgctcgacag g
//
LOCUS       DAOSIF010000599         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58502_length_1756_cov_2.388595, whole genome shotgun sequence.
ACCESSION   DAOSIF010000599 DAOSIF010000000
VERSION     DAOSIF010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58502_length_1756_cov_2.388595"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1633)
                     /gene="putP"
                     /locus_tag="PLT35_14270"
     CDS             complement(125..1633)
                     /gene="putP"
                     /locus_tag="PLT35_14270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HOG30439.1"
                     /translation="MNIHVVVTFALYLLLMLGIGIYFSRKTLDLGDYYLGGRRMNKWV
                     VALSAQASDMSGWLLMGLPGAIYLSGLSESWIGLGLAAGTYLNWRFVARRLRRFSHAC
                     GDAITIPDFLSNRFHDASGVVRMVSAFIILAFFLFYTASGFVACAKLFETTFGLRYDL
                     ALTLGAFVIVSYTLMGGFSAVCWTDVIQGSIMFFAIVAVPVACVFNAGGAGEALALIK
                     GVNPDLLSFTTSASTGAGVGALGLVSSLAWGLDYFGMPHILVRFMAIDDEKNIAASRR
                     IAMAWVLVSLAAAILVGLLGHAFVAKSGVVVDDPEKVYMLMIKGLFHPLLIGVLMSAI
                     LAAIMSTADSQLLVSASAFVNDFYKTTLRRSAGTRELMWASRLTVAAVAVLAAIMASR
                     PDSDFVKAVMKLVSFAWGGFGSAFGPVILLALFWRRTTLAGAVSGMVAGAATAFIWKF
                     KLSALAAQHPVFGLYELAPGFAICLFVAVVVSLLGKPPSAETVEESDRMAAG"
BASE COUNT          358 a          551 c          592 g          255 t
ORIGIN      
        1 gtgctcggag agcccgccct acctcgtaga ctcaccgttt ggcggtgatt tcggggtagg
       61 gcgcgatctc cgagcgcgcc gtttccctgg ttcccggcca tattgagaat tgctgtgggc
      121 ggattcagcc tgcggccatg cggtcggact cctcgacagt ttcggccgag ggcggtttgc
      181 cgagaaggct cacgacgacg gcgacgaaca ggcagatcgc aaaccccggg gccagttcgt
      241 aaaggccgaa gacggggtgt tgcgccgcca gggcgctgag cttgaacttc cagatgaagg
      301 cggtcgcggc gcccgccacc atgcccgaga ccgcgccggc cagcgtcgtg cgccgccaga
      361 agagcgccag caggatgacg gggccgaagg ccgagccgaa gccgccccag gcgaagctca
      421 ccagcttcat caccgctttg acgaaatcgc tgtcgggtcg ggaggccatg atcgccgcca
      481 gcacggcgac ggccgccacc gtcagccgac tcgcccacat cagctcgcgc gtgccggcgc
      541 tgcggcgcag cgtcgtcttg tagaagtcgt tcacgaacgc cgaagcggag acgaggagct
      601 gcgaatcggc cgtgctcatg atcgccgcga ggatggccga catcaggacg ccgatcagca
      661 gcgggtggaa cagccctttg atcatcagca tgtagacctt ctccggatcg tcgacgacca
      721 ccccggactt cgccacaaag gcatggccga gaagacccac cagaatggcg gccgcgaggg
      781 agacgagaac ccacgccatc gcgatccggc gcgaggcggc gatgttcttc tcgtcgtcga
      841 tcgccatgaa gcgaacgagg atgtggggca tgccgaagta gtccaggccc caggcgaggc
      901 tggacaccag cccgagggcg ccgacgcccg cccccgtgct ggccgacgtg gtgaagctca
      961 gcaaatcggg attgacgccc ttgatcagcg cgagagcctc tccggcccct cccgcgttga
     1021 acacgcaggc cacgggcacg gcgacgatgg cgaagaacat gatggagccc tggatgacgt
     1081 ccgtccagca gacggcggag aagccgccca tcagcgtgta ggagacgatg acgaaggcgc
     1141 ccagcgtcag cgccagatcg tagcgcaggc cgaaggtcgt ctcgaacaac ttcgcgcagg
     1201 ccacgaaccc ggaggccgtg tagaacagga agaacgcgag gatgatgaag gcggagacca
     1261 tgcgcacgac gcccgacgcg tcgtggaagc ggttcgacag gaagtcgggg atggtgatcg
     1321 cgtcgccgca ggcgtgggag aagcgccgca gccgccgggc cacgaagcgc cagttcaaat
     1381 acgtccccgc cgccaggccg aggccgatcc aggattcgct caggcccgag aggtagatgg
     1441 cgcccgggag gcccatcagc agccatccgc tcatgtcgga ggcctgcgcg ctgagcgcca
     1501 cgacccactt gttcatccga cgtccgccca gatagtagtc gcccagatcg agggtcttcc
     1561 tggagaagta gatgccgatc cccagcatca gcagcagata gagcgcgaac gtgacgacga
     1621 catggatgtt catggtgggg ttcctcgata accgggcggc tggtgatttg tctgaatatt
     1681 gccacaagat ccttcgcgtt gaaagtcaaa aggcaatcga caggagaacg tgggtgtcgt
     1741 tgagttgtcc accggt
//
LOCUS       DAOSIF010000600         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58630_length_1753_cov_3.981743, whole genome shotgun sequence.
ACCESSION   DAOSIF010000600 DAOSIF010000000
VERSION     DAOSIF010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58630_length_1753_cov_3.981743"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>495
                     /locus_tag="PLT35_14275"
     CDS             <1..>495
                     /locus_tag="PLT35_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG30440.1"
                     /translation="ERAYELNPSDDLARRLDDARARASARRLPPEYAAISQAPQITRG
                     DLAALLGVRVGWLMAAPRARAGAVAIDVRGHWASAWILGMIRAGVMDVLPNHAFQPRA
                     LVRRADLAVAVSRVLALAGPTASAPGRARPEITDVGPDHLRYADIEAAVSAGILTLDR
                     GLFRP"
     assembly_gap    496..505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            600..1166
                     /locus_tag="PLT35_14280"
     CDS             600..1166
                     /locus_tag="PLT35_14280"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG30441.1"
                     /translation="MGALTLANQMTLLRLGLVPAFVILLLYGRVGWAFAVFVTAGITD
                     ALDGLVARRRNQRTALGALLDPMADKLLLVSAFVVLTLPGLGLVNRLPVWLTILVISR
                     DVAIVVTVAIISLTVGHRSFPPSIYGKIATGVYIVTVAIVLLFNYLGRRSVLVDVGVW
                     TSLAITVVSGLHYTAHVRRVLNEPPASP"
     gene            complement(1141..>1753)
                     /locus_tag="PLT35_14285"
     CDS             complement(1141..>1753)
                     /locus_tag="PLT35_14285"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG30442.1"
                     /translation="ARGNSAYDRYYGDPLVHPQDEGYWGNVNPIGPRGVYDEAKRFAE
                     AMTVAYHRYHGLDAKIVRIFNTFGPRMRVRDGRAVPNFITQALRGQDLTVYGDGSQTR
                     SLCYISDLVDGVIRLMHSDANGPVNIGNPRELTVEALARLVVRLTGSSSRIVHQALPV
                     DDPRVRKPDITRAETILGWRPRVSLEDGLARTIEYFREKLAAR"
BASE COUNT          230 a          604 c          623 g          286 t
ORIGIN      
        1 cgagcgcgcc tatgaactga acccgtccga cgatctcgcg cggcgcctgg acgacgcgcg
       61 tgcgcgggcg tccgccaggc ggcttccgcc cgagtacgcc gcgatttcgc aggcgccgca
      121 gatcacgcgc ggcgacctgg ccgcccttct cggtgtgcgc gtcggctggc tcatggcggc
      181 cccccgcgcg agagcgggtg ccgtggcgat cgacgtccgc ggccactggg cgtcggcgtg
      241 gattctcggg atgattcggg ctggcgtgat ggacgtcttg ccgaaccacg cgttccagcc
      301 ccgggccctc gtccgccggg cggatctcgc cgtggcggtg agccgggtgc tggcgctggc
      361 cgggccgaca gcgtcagcac cgggacgcgc caggccggaa atcacggatg tggggccgga
      421 ccacctgcgg tacgcggaca tcgaggccgc ggtgtcggcc gggatcctga cgctcgatcg
      481 ggggctgttc aggccnnnnn nnnnncaggc cgtcgcgggc cgtcagcggc gaagaggcca
      541 tcgaggccat ccggcgcctc gagcgcgcgg caccgccgcc ggacagggga cgctgaccca
      601 tgggcgcgct gacgctcgcc aaccagatga ccttgctcag gctcggcctg gtgccggcct
      661 tcgtgatcct gctgctctac gggcgcgtcg gctgggcgtt cgccgtcttc gtgaccgcag
      721 ggatcaccga tgcgctcgac gggctggtgg cgaggcggcg aaaccagcga accgccctcg
      781 gcgcgctgct cgatccgatg gcggacaagc tgctgctggt gagcgccttc gtcgtgctga
      841 cgctgcccgg cctcggcctc gtcaaccgcc tcccggtctg gctgacgatc ctcgtcatca
      901 gtcgcgacgt cgccatcgtc gtgaccgtgg ccatcataag cctgacggtg ggccatcgct
      961 cgttcccgcc gagcatctac ggcaagatcg cgaccggcgt ctacatcgtg accgtcgcca
     1021 tcgtgctcct gttcaactac ctgggacgga gatccgtgct ggtggacgtc ggcgtctgga
     1081 cgagcctggc catcaccgtg gtttcggggc tgcactacac ggcgcacgtg cgccgggtcc
     1141 tcaacgagcc gccagcttct ccctgaagta ctcgatcgtg cgggcgagcc cgtcctccag
     1201 ggagacccgc ggccgccagc ccaggatcgt ctcggcccgg gtgatgtccg gcttgcggac
     1261 cctggggtcg tcgaccggca gcgcctggtg cacaatccgg ctggacgatc cggtgaggcg
     1321 gacgaccagc cgcgccagcg cctcgacggt gagttcgcgg ggattcccga tgttgacggg
     1381 cccgttcgca tcggagtgca tcaggcgaat gaccccgtcg acgaggtccg agatgtagca
     1441 caggctgcgc gtctggctgc cgtcgccgta gacggtgaga tcctggccgc gaagggcctg
     1501 cgtgatgaag ttcggcaccg cccgcccgtc gcggacccgc attctcggcc cgaaggtgtt
     1561 gaagatacgc acgattttcg cgtcgagccc gtggtaccgg tggtacgcga cggtcatcgc
     1621 ctcggcgaag cgcttggcct cgtcgtacac gcctcgcggc ccgatgggat tcacgttgcc
     1681 ccagtagccc tcgtcctggg ggtggaccag cgggtcgccg tagtagcggt cgtacgcgga
     1741 gttgccccgc gcg
//
LOCUS       DAOSIF010000601         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58880_length_1748_cov_3.415830, whole genome shotgun sequence.
ACCESSION   DAOSIF010000601 DAOSIF010000000
VERSION     DAOSIF010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58880_length_1748_cov_3.415830"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1189)
                     /locus_tag="PLT35_14290"
     CDS             complement(35..1189)
                     /locus_tag="PLT35_14290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG30443.1"
                     /translation="MNEDRAGRYHRLRRRAAAESAAWTASWLIALLLTGWSRSLSEAA
                     ERMTAGLPAALRLPGAVIVWGLAVAAVHEAGRLPFACYNGFILERRYGLSRQQAAAWL
                     ADHLKSGLVGLAFALAASVWVAACLQRWPDGWWLPAWAGAAAAAVAAAWAAPVLLLPL
                     FCALQPLRDEALRRRLLALAARAGVPAIDIVEWRLSGRSSRANAALVGLGRTRRIVVS
                     DTLASGYRSEEVEAVVAHELAHRAHRDIWFALGIDAAQTAAALLASDAALRAAAGPLS
                     LAGAPVAAGLPIVALVSLAVGLLSAPAAHALSRARERRADRYALDLTGNADAFARAVR
                     RLGAHNLAEEHPGPLARAFFHTHPPVEERLAAARAWAAARGAGPATGAGT"
     gene            complement(1293..>1748)
                     /locus_tag="PLT35_14295"
     CDS             complement(1293..>1748)
                     /locus_tag="PLT35_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOG30444.1"
                     /translation="PRRRPVEVTYTIRAPRRAKLEIETVNGSITVNGAAAALKAESTN
                     GSIDGTDLSGDVKAETTNGSIKIRMAALGDGGVALETTNGSVDLRLPGDAKATLSARS
                     VNGGISVADLPFERTGEAGRRKLDGAINGGGAPLRLETVNGRIRVGRAQ"
BASE COUNT          222 a          696 c          626 g          204 t
ORIGIN      
        1 gacggctgcc gcgacatgcg cggcgagctg gtggtcacgt cccggctccc gtagccggcc
       61 ccgcgccgcg cgcggccgcc cacgcccggg cggccgccag cctctcctcg accggcggat
      121 gggtgtggaa gaacgccctc gccagcggcc cgggatgttc ctcggccagg ttgtgcgcac
      181 cgagcctgcg cacggccctc gcgaacgcgt cggcattccc cgtgagatcg agggcgtacc
      241 gatccgcgcg ccgttcgcgc gcccgcgaca gggcgtgcgc cgccggcgcc gacaggaggc
      301 cgacggcgag cgagaccagg gcgacgatcg gaagcccggc ggcgacggga gcgcccgcca
      361 gcgacagcgg gcccgccgcc gcccgaaggg ccgcatccga cgcgagcagt gcggccgccg
      421 tctgggcggc atcgatgccg agcgcgaacc agatgtcccg atgggcccgg tgcgccagct
      481 catgcgccac cacggcctcg acttcctcgc tccgatagcc cgaggccagc gtgtcggaca
      541 cgacgatgcg gcgggtccgg ccgagcccca cgagcgccgc gttcgcgcgc gagctccggc
      601 cgctcagccg ccactcgaca atgtcgatcg ccggcacgcc ggcgcgcgcc gccagcgcga
      661 gcaggcgccg gcgcagcgct tcgtcgcgca gcggctggag cgcgcagaag agcggcagca
      721 gcagcacggg ggcggcccag gcggcggcca ctgccgcggc ggcggccccc gcccaggccg
      781 gcagccacca gccgtccggc cagcgctgca ggcacgcagc cacccagacg gaggcggcaa
      841 gcgcgaaggc cagcccgacc agtccggact tgagatgatc cgccagccac gctgccgcct
      901 gctgccgcga cagcccgtag cggcgctcga ggatgaaccc gttgtagcac gcgaacggca
      961 gtctgccggc ttcgtggact gccgccacgg ccagccccca gacgatgacg gctccgggca
     1021 ggcgcaaggc cgcgggcaga ccggccgtca tccgctcggc ggcctcgctc aggctcctgg
     1081 accacccggt gagcaggagc gcgatgagcc aggacgccgt ccacgccgcg ctctccgcgg
     1141 cggcgcggcg ccggagacgg tggtagcggc cggccctgtc ctcgttcatg cctgcccgtt
     1201 tcaggcgcct gccgcgccgc aggcgcgctc acggaagccg gctcgccggc cacgcacgcg
     1261 gggaccggtc ccgaccgggc ccgtccgcgc ccctactggg cgcgcccgac gcggatgcgc
     1321 ccgttcaccg tctcgagccg cagcggcgcg ccgcccccgt tgatggcgcc atccagcttc
     1381 cggcggccgg cttcccccgt ccgctcgaaa ggcaggtcgg ccacgctgat cccgccgttg
     1441 acgctgcgcg ccgagagggt cgccttcgca tcgcccggca ggcggagatc caccgacccg
     1501 ttggtcgtct cgagggccac gccgccgtcg ccgagcgccg ccatccggat cttgatcgac
     1561 ccgttggtcg tttccgcctt gacgtccccg ctcaggtccg tgccgtcgat actgccgttg
     1621 gtggattccg ccttcagcgc cgctgcggcc ccgttgacgg tgatgctccc attgacggtc
     1681 tcgatctcga gcttcgcgcg gcgcggcgcc cggatggtgt aggtcacctc gacgggccgc
     1741 ctccgcgg
//
LOCUS       DAOSIF010000602         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58882_length_1748_cov_3.233904, whole genome shotgun sequence.
ACCESSION   DAOSIF010000602 DAOSIF010000000
VERSION     DAOSIF010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58882_length_1748_cov_3.233904"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /gene="amrA"
                     /locus_tag="PLT35_14300"
     CDS             <1..1099
                     /gene="amrA"
                     /locus_tag="PLT35_14300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOG30445.1"
                     /translation="VFDSSLHRGEHSVEVQVPFVQHLFPKASIVPVVVGAPDPDSCRR
                     FGKALASLAGARRVLIVASSDLSHYPAKGIAAGVDRQTLEALANLRTDGFDSSPARAV
                     TLQMPGVSTCACGEGPIRVAMEAARALGALRGTVISYANSGDTIVGDPDRVVGYGAVV
                     FGRGEPGADITALARPPADPRGSLDAADKRVLLRLARESLTRLLETDTLPLPRGGSPK
                     LLREAGAFVTIRKRGELRGCIGRIQPEGPLIGLVARLGLESALKDTRFQPVQRSELKD
                     LEFEVSVLTLPVPVASPDDVVPGRDGVILRVGDRSAVFLPQVATEQGWGREELLDRLA
                     EKAGLRPRAWREKNASLLTFQADVFSESAFK"
     gene            1096..>1748
                     /locus_tag="PLT35_14305"
     CDS             1096..>1748
                     /locus_tag="PLT35_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30446.1"
                     /translation="MTLLVLAGVLSMLAQVVILRELVSALFGVELLYVLALGTWLVGT
                     AAGSWAAARAAARPRVLAAGFLALGVFVPAGLVLVRAAGRILGAMPGAYLPFPAQVLL
                     IGAATLPAAAACGALFPPLAAAGGRRSRSVGRAYAIESAGAAAGGALVTAAMWLGVPT
                     FAVAMVAAALAFAAAAIAVMRRPAAAAALAAIAAAGAVALPRAGPWDARLARWTHPAL
                     "
BASE COUNT          179 a          640 c          662 g          267 t
ORIGIN      
        1 cgtcttcgac tccagcctcc atcgcggcga gcactcggtg gaggtgcagg tgccgttcgt
       61 gcagcacctc ttcccgaagg cgtcgatcgt cccggtcgtg gtcggcgcgc ccgacccaga
      121 ctcgtgccgc cggttcggga aggcgctggc gtcgctcgcc ggagcccgca gggtgctcat
      181 cgtggccagc tcggacctgt cgcactatcc ggcgaaaggc atcgccgccg gcgtcgaccg
      241 ccagacgctg gaggccctcg ccaacctgag gacggatggc ttcgacagct cgcccgcgcg
      301 cgccgtgacg ctgcagatgc ccggcgtctc gacctgcgcc tgcggcgagg ggccgatcag
      361 ggtggcgatg gaagcggcgc gcgcgctcgg cgccctgcgc ggcacggtga tcagctacgc
      421 caactccggc gacacgatcg tcggcgaccc cgaccgcgtc gtcgggtacg gcgcggtggt
      481 gttcggccgg ggcgagccgg gtgcggacat caccgccctg gcgcgtccgc cggccgaccc
      541 gcgcggcagc ctcgacgcgg ccgacaagcg cgtgctcctc cgtctcgcgc gcgaatcgct
      601 cacgaggctg ctcgagaccg acacgctgcc gctgcccagg ggcggctcgc cgaagctgct
      661 gcgcgaggcc ggcgcgttcg tcacgatccg gaagcgcggc gaactgcgcg ggtgcatcgg
      721 ccgcattcag cccgaaggcc cgctgatcgg gctcgtcgcc aggctcggcc tcgaatcggc
      781 gttgaaggac acgcggttcc agccggtcca gcgcagcgag ctgaaggatc tcgagttcga
      841 ggtgtcggtg ctgacgctgc ccgtccccgt cgccagcccc gacgacgtcg tcccggggcg
      901 cgacggcgtc atcctgcggg tcggggaccg gtcggccgtg ttccttccgc aggtggcgac
      961 cgagcaggga tgggggcgcg aggaactgct cgatcgcctc gcggagaagg cggggcttcg
     1021 gccgcgcgcg tggcgggaga agaacgcgtc gctcctgacg ttccaggcgg acgtgttctc
     1081 tgagtccgcg ttcaagtgac cctgctcgtc ctcgccggcg tgctgtcgat gctggcgcag
     1141 gtcgtgatcc tgcgggagct cgtgtcggcg ctgttcgggg tcgaactcct ctacgtgctt
     1201 gcgctcggga cgtggctcgt cggcaccgcg gcaggctcgt gggccgcggc gcgcgccgcg
     1261 gcgcggcctc gggttctcgc cgccggcttc ctggccctgg gcgtgttcgt gccggcaggg
     1321 ctcgtcctcg tccgcgcggc cggccggatc ctcggcgcca tgcctggagc ctacctgccg
     1381 tttcccgcgc aggtcctgct gatcggggcg gccacgcttc ctgcggctgc cgcctgcggc
     1441 gcgctgtttc cgccgctcgc ggcggccgga ggccgccgga gccgcagcgt gggccgtgcc
     1501 tacgcgatcg agagcgccgg cgccgcggcg ggcggggcgc tcgtgacggc agcgatgtgg
     1561 ctcggcgttc cgacgttcgc cgtggcgatg gtcgctgcgg ctctcgcgtt cgccgccgcc
     1621 gcgatcgccg tgatgcggcg ccccgccgcc gccgccgcgc tggccgcgat cgcggcggct
     1681 ggggctgttg cgcttccgcg cgccggcccc tgggacgccc gcctcgccag gtggacgcat
     1741 ccggcgct
//
LOCUS       DAOSIF010000603         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_58917_length_1747_cov_3.779551, whole genome shotgun sequence.
ACCESSION   DAOSIF010000603 DAOSIF010000000
VERSION     DAOSIF010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58917_length_1747_cov_3.779551"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1504)
                     /locus_tag="PLT35_14310"
     CDS             complement(203..1504)
                     /locus_tag="PLT35_14310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG30447.1"
                     /translation="MTRRHARAGGWALAAAAVTAVAGTGLATRGASQPKPLVTTAAVT
                     RGEIVAEISASGTLAAVSTVSVGSQVSGRIQALHADFNDVVRRGQVLAELDPSLLRAE
                     VKQAGASLLGAEAAVERLAVERDAARLALDRTERLFERQLVAALDVEAAQVALRSLEA
                     DIRSAQASVARAKAALNQAQVNLAHTVIASPIDGTVISRAVEVGQTVAAGMSAPTLFT
                     LAADLTRMRVSANIDESDVGAVAEGQRATFRVDAYPGEAFTGTVEQVRLEASTSQNVV
                     TYEAIVAVDNRALKLKPGMTATVAIEADRRADALQVPNGALRVRPSAEALAALGVSGP
                     DAAGWQAVCRGVPGCVTVWRQRGLALEPVAVGAGITDGATTEVLGAALAEGDLVVTAI
                     GVPTAAARAATASGATSGTANPLLGSQPRGPFPGGGGPPPR"
BASE COUNT          238 a          672 c          608 g          229 t
ORIGIN      
        1 gcgaaggtgt tgcggacgag atagaaggcc gcgtagccga cgaagatgcc gatgaagacc
       61 tgccgccgca accgcctgta ggccggatcg accagatcgg gcggcagcgg atcggccggc
      121 gcggcgctct tgaggattcc aatcatgggg cgagagtcta gcacgaacgc gcgtgccgct
      181 ctcgcccccc cccggagccg agctagcgcg gcggaggccc gccgccgccg gggaacggtc
      241 cgcggggctg gctcccaagg agcggattgg ccgtgccgga cgtcgcgccg gacgccgttg
      301 ccgcccgtgc cgcagcggtc ggaacgccga tcgccgtgac gacgaggtcg ccctcggcga
      361 gcgccgcccc gaggacctcg gtggtcgcgc cgtccgtgat gcccgcgccg acggcgaccg
      421 gctcgagcgc cagcccccgc tgccgccaca cggtcacgca gcccggaacg ccccggcaga
      481 ccgcctgcca gccagctgca tccggcccgg acacgcccag ggcggccagg gcttccgccg
      541 acggcctgac gcgcagcgcg ccgttgggca cctgcagcgc gtccgcgcgc cggtccgcct
      601 cgatggcgac ggtggccgtc atgcccggct tcagcttcag cgcgcggttg tccacggcga
      661 cgatcgcctc gtaggtgacg acgttctgtg aggttgacgc ctccagacgc acctgctcga
      721 cggtgcccgt gaacgcctcg cccggatagg cgtccacgcg aaacgtggcg cgctgccctt
      781 ccgcgacggc gcccacgtcg gactcgtcga tgttcgcgct cacgcgcatg cgggtcaggt
      841 cagctgcgag cgtgaagagc gtcggcgccg acatgcctgc cgccaccgtc tggccgacct
      901 ccaccgcgcg cgagatcacc gtcccgtcaa tcggcgacgc gatgacggtg tgcgccaggt
      961 tgacctgggc ctgattcagc gcggccttcg cccgagcgac cgacgcctgc gccgaccgga
     1021 tgtcggcctc gagcgaccgg agcgccacct gggcggcttc gacatcgagg gccgccacca
     1081 gctggcgctc gaacagcctc tccgtcctgt ccagcgcaag gcgcgcagcg tcccgctcga
     1141 ccgccaggcg ctcgacggcg gcctcggcgc cgaggaggct cgcgccggcc tgcttgacct
     1201 cggcccgcag cagggacgga tcgagttctg ccagcacctg ccctcgccgc acgacgtcgt
     1261 tgaaatcggc atgcagcgcc tggatccgcc ctgacacctg gctgccgacg ctcaccgtgc
     1321 tcacggccgc tagggtgccc gaggcgctaa tctccgccac gatctcgccg cgtgtcacgg
     1381 ccgccgtcgt caccagcggc ttcggctggc tggcaccgcg ggtcgccagg cccgtccccg
     1441 caacggccgt caccgctgct gcagcgagcg cccatccccc cgcgcgtgcg tgtcgtcgag
     1501 tcatcgccgg acctccccga gcggttcgcg ctctactcgg atctacgctc gacccgaggc
     1561 acggcggacg caagagtgtg aaaggtgtgt ggcgggcggc tgacggccgc agaagggccg
     1621 ggccggcggg ccccggccct tccgctgtcc gccgcatggg gacgagaggc ctacttcccg
     1681 agcgggtacg cgaaccggta ctgccccgac ccgacctcga cgaggacggc cgtcccttcc
     1741 tggcgcg
//
LOCUS       DAOSIF010000604         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_59274_length_1740_cov_3.932344, whole genome shotgun sequence.
ACCESSION   DAOSIF010000604 DAOSIF010000000
VERSION     DAOSIF010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59274_length_1740_cov_3.932344"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLT35_14315"
     CDS             complement(<1..378)
                     /locus_tag="PLT35_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30448.1"
                     /translation="MSVKFTVARRTLTGLLSAALLVAFALPVSAQHRARLSQDLAEKL
                     AAQSGATLHVISNLEGERLQKAAARYSLRIHKRLDGAAVLQGTAAQIDRAADDPDFGH
                     LALDAPVTAQMAVTREAIGALLAR"
     gene            complement(395..658)
                     /locus_tag="PLT35_14320"
     CDS             complement(395..658)
                     /locus_tag="PLT35_14320"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG30449.1"
                     /translation="MGIDQRTFDALDRIEARSLSLAQACTALHVSRRTVYYLIKEGRL
                     ATVRTPLGSQRVLVESLRAVRMEREARLADRGSDGFRRVSCGN"
     gene            1267..>1740
                     /locus_tag="PLT35_14325"
     CDS             1267..>1740
                     /locus_tag="PLT35_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG30450.1"
                     /translation="MTRAGRLLISLALVGTAATTSVPRGRHFARLAGPVSVPAEAAAA
                     TPAATPAAPLAAPAQTAVRPRRGGSLVATLRSEPRTLNPLTGRDFASTLVASLTHARL
                     LRINRATQQLEPWLAESYACSADGLSCMLKLRRGVTFSDGAPFTSADVVFSFRAVY"
BASE COUNT          241 a          638 c          631 g          230 t
ORIGIN      
        1 gcgggcgagc agcgcgccaa tcgcctcgcg cgtcacggcc atctgggcgg tgaccggcgc
       61 gtcgagcgcc aggtgcccga agtcgggatc gtcggccgca cggtcgatct gcgcggcggt
      121 gccctggagc acggcggccc cgtcgagccg cttgtggatc ctgagcgagt aacgcgcggc
      181 agccttctga agccgctcgc cctcgaggtt cgagatgacg tggagggtgg cgccgctttg
      241 cgccgccagc ttctcggcca ggtcctgcga gagccgcgcc cggtgctgcg ccgacacggg
      301 cagggcaaac gcgacgagca gggccgcaga caagaggccg gtcagggtcc gacgcgcgac
      361 ggtgaacttg acgctcatga cgctcctccg cgggtcagtt cccgcaggac actcggcgaa
      421 atccatcaga ccctctatcg gccagccgcg cctcccgctc catgcgcacg gccctgagcg
      481 actcgaccag cacccgctgc gatccgagtg gcgtgcgcac ggtggcaagc cggccttcct
      541 tgatgaggta gtagacggtg cggcgcgaaa cgtgcagcgc cgtgcaggcc tgcgccagcg
      601 acaggctccg cgcctcgatc cggtcgagcg cgtcgaaggt ccgctggtcg atccccacgc
      661 tgcacctccc gccggcacaa gagaggagcg aaaagagtgc cagggcgtgg ccggaaacga
      721 ggctcgtgtc gatgcgcgca agtattgcaa aatgagacac ttgcgggcgg actggcggga
      781 acgggccttg cctggctaag gccctcacgg ccaacgggtt ggccggccgg ccagcctgct
      841 gtccgaggcg aaaggcgcgc ggattcacgg ggttgcacgg atttcgtgcc aggcccgcac
      901 gattcggtgc aagaacgcgc accggagccg ctcacggttc tgtgaggccg ctgacagaac
      961 cgtgccggca gcgcccgcgg cgcacggtcc tgcccctgca ggccgccatc gacgccggtc
     1021 ggcagccgga cggccccgcc ggacggaaac cctcgacgcc tgctcccggc gacgggccat
     1081 ccgccgctga cagaagcacg ccgtgcccgt gcggtcgact ggaggcgggc cggtctcgtc
     1141 gacggccacg cgggcggagc gcgcgagggc tttcagccgg gccgggggcc gcccggcggc
     1201 ccggccgagc gcgtccctca tcacacgggc cggtcgggcc gataaccttc acgtgggact
     1261 tgccaggtga cgcgcgcggg acgcctcctc atctccctgg ccctcgtcgg cacggccgcg
     1321 acgacgagcg tgccccgcgg gcggcacttc gctcgcctgg cggggccggt ctcggtcccg
     1381 gcggaggccg cggcggcaac gccggccgcg acgccggcgg cgcccctcgc cgcgcccgcg
     1441 cagacggccg tccggccccg gcgcggcggg tcgctcgtgg cgacgctgcg cagcgagccg
     1501 cgcacgctga acccgctcac cggccgcgac ttcgcctcga cgctcgtggc gtcgctcacg
     1561 cacgcccgcc tgctgcgaat caaccgggcg acacagcagc tggagccgtg gctggccgag
     1621 tcgtacgcct gctcggccga cggattgtcg tgcatgctca agctgcgccg cggcgtgacg
     1681 ttctcggatg gcgcgccgtt cacctcggcc gacgtcgtct tctcgtttcg ggcggtctac
//
LOCUS       DAOSIF010000605         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_59347_length_1739_cov_2.588480, whole genome shotgun sequence.
ACCESSION   DAOSIF010000605 DAOSIF010000000
VERSION     DAOSIF010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59347_length_1739_cov_2.588480"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..485)
                     /locus_tag="PLT35_14330"
     CDS             complement(120..485)
                     /locus_tag="PLT35_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30451.1"
                     /translation="MRYLWPWWRRLLVRALVLVLALGAAPLNCLAAQPADPPPPAPAL
                     AAAIQQAVQLEVGTMTKVSPNAAQQSGASAAEPARFFKTKAGVVALVLLAAGTGYALY
                     STSHDRIKSPKVPYGGTWK"
     gene            631..>1739
                     /locus_tag="PLT35_14335"
     CDS             631..>1739
                     /locus_tag="PLT35_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOG30452.1"
                     /translation="MRCGAQPGLAEAPEGGGGAACRAASWSKRRRAATAAGAAAAFLL
                     ISALGWLGRQALAPRGERAGSVDLGALPPGVSRDALNLVLITLDTARADHVSAYGSTT
                     VQTPALDRLASDGVLFEQAGTAAPLTLPAHSTILTGRFPPEHGVRDNGGFYLSPDQTT
                     LAEMLSAQGFATGGFVAAYVLDGKWGIGQGFGTYFDDFDLGGRRGRSLGDIQRPANEV
                     ADKALPWIDSVKDRRFFAWLHFYDPHTPYEPPEPYRTQYQGHPYRGEMAFTDSQVGRV
                     VDHLASNGLLDKTVVVAIGDHGESLGEHGEDAHGFFIYEAAARVPFIIRAPFSAARGR
                     RVADPVRTVDVVPTVLDLLGLPPAASVSGRSLVPL"
BASE COUNT          251 a          628 c          608 g          252 t
ORIGIN      
        1 cagcaccgcg ccctgctccc caatgatccg gccggacagc gtgccggtct gggtctgcgc
       61 catggcgccg gccgtgaaga ggagcacgaa gaccgccatc acaatcgagg cgcgcgtctt
      121 catttccagg tccctccata cggcaccttc ggcgacttga tgcgatcgtg ggacgtggaa
      181 tacagcgcgt atcccgtgcc tgctgccagc agcaccagcg cgacgacgcc ggccttcgtc
      241 ttgaagaaac gggcgggctc ggccgccgag gcgccagatt gctgcgcggc attgggcgac
      301 accttcgtca tcgtgcccac ctccagctgc acggcctgct gaatcgccgc cgcaagtgcc
      361 ggtgcgggtg gggggggatc ggcgggctgc gccgccaggc aattcagagg ggcggcgccc
      421 agcgccagca cgagcaccag ggctcgcacc agcaggcgtc gccaccatgg ccagaggtac
      481 ctcacgctcc ctcctttcgc ggatgaaccc gcgcacgcac gtacacagcc gccggagccg
      541 ccggagccgg ctgggcgcgg cacggcgacg gcagcatatc agaagagcgg cgttgacgca
      601 ttgccgattt tcaaccacga tctgctcagt atgagatgcg gcgctcaacc cgggcttgcc
      661 gaggcgcctg aggggggggg cggggcggcg tgtcgagcgg cgagctggtc gaagcggcgg
      721 cgggcagcca cggcagccgg cgccgccgcg gcgttcctgc tgatcagtgc gctcggctgg
      781 ctgggccggc aggcgcttgc gccgcgcggc gagcgggccg gttcggtcga cctcggcgcg
      841 ctgccgccag gcgtctcgcg cgacgccctg aacctggtgc tcatcacgct cgacacggct
      901 cgcgcggacc atgtgagcgc ttacggctcc acgacggtcc agacgccggc cctcgaccgc
      961 ctggccagcg acggcgtgct gttcgagcag gccgggacgg cggccccgct gaccctgccc
     1021 gcgcactcca cgatcctgac gggcaggttc ccgccggagc atggcgttcg ggacaacggc
     1081 gggttctacc tgtcgcccga ccagacgacg ctggccgaaa tgctgagcgc gcagggcttc
     1141 gcgacgggcg gcttcgtcgc cgcctacgtg ctcgacggca agtgggggat cggccagggc
     1201 ttcggcacct acttcgacga tttcgatctc ggcggccggc gggggcggtc gctcggcgac
     1261 atccagcgcc cggccaacga ggtggcggac aaggcgctgc cctggattga cagcgtgaaa
     1321 gaccgccggt tcttcgcctg gctgcacttc tacgatccgc acaccccgta cgagccgccc
     1381 gagccctacc gcacccagta ccagggccac ccgtatcgcg gcgagatggc cttcaccgac
     1441 tcgcaggtcg ggcgcgtcgt cgaccacctc gcctcgaatg gcctgctcga caagaccgtc
     1501 gtggtggcca ttggcgacca cggcgagagc ctcggcgaac acggcgagga cgcccacggc
     1561 ttcttcatct acgaagcggc cgcgcgcgtc cccttcatca tccgcgcgcc gttctcggcc
     1621 gcgcgcggca ggcgggtcgc cgatccggtc cggacggtgg acgtcgtgcc gacggtgctc
     1681 gacctgctgg gtttgccgcc ggccgcctcg gtctcgggcc gcagcctcgt gccgctcat
//
LOCUS       DAOSIF010000606         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_59557_length_1734_cov_4.106611, whole genome shotgun sequence.
ACCESSION   DAOSIF010000606 DAOSIF010000000
VERSION     DAOSIF010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59557_length_1734_cov_4.106611"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLT35_14340"
     CDS             complement(<1..353)
                     /locus_tag="PLT35_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30453.1"
                     /translation="MTVFVYAAIVFGVLAFAVGTAARAVQYARQPMHLRWELYPVPHE
                     RADRAAHGGSYFEQSEWWRQPRQRNLRGELAAMLPEMLLLKALREFNRGLWYRSFPFH
                     LGLYLLAAAAVALLAL"
     gene            complement(376..693)
                     /locus_tag="PLT35_14345"
     CDS             complement(376..693)
                     /locus_tag="PLT35_14345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOG30454.1"
                     /translation="MPIVELNGRPYHVDDNGFLQDPQIWNDDVARDFATTEGIAEMTD
                     EHWKLVRYLREYYLQFGIAPMVRKLCKETGLKLARVYELFPSGPANGACKVAGLPKPT
                     GCV"
     gene            complement(720..>1734)
                     /locus_tag="PLT35_14350"
     CDS             complement(720..>1734)
                     /locus_tag="PLT35_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG30455.1"
                     /translation="RRQLTRFFNGTDPDRLVFTYNSTDALNLALFGMLSPGDHAITTT
                     VEHNSVLRPLHHLSQYHGVEVDRVPFDGRGFVDPGDIAARFKPNTRVVAMNHGSNVIG
                     TVQPVAEVGRLCRERGIRFVVDSSQTAGKIPIDVQAMHVDVLAFTGHKSLMGPTGIGG
                     LYVQPHVDIRHTRAGGTGVRSAHPFHLDEYPWRLEYGTPNVLGIAGLHAGVSWLEQQG
                     LAAIEAHEMRLLRRLVEGLRGIPNVTMYCQDDLADHIAVMVFNVDGFEAGNTGTILDV
                     DHNIACRTGLHCAPLVHAQLGLDAIKGAVRFGIGPFNTDAHIEAAIGAVGEIAAARAA
                     KAR"
BASE COUNT          268 a          589 c          611 g          266 t
ORIGIN      
        1 agggcgagca gggcgacggc ggcggcggcc agaaggtaca gccccaggtg aaacgggaac
       61 gagcggtacc acagcccccg gttgaactcc cgcagcgcct tcaacaggag catctccggc
      121 agcatcgccg ccagctcgcc ccgcaggttc cgctgccggg gctggcgcca ccactcgctc
      181 tgctcgaaat acgagccgcc gtgcgccgcg cgatcggcgc gctcgtgggg cactggatac
      241 agctcccacc tgaggtgcat cggctgccgg gcgtactgca cggcgcgggc ggcggtcccc
      301 accgcgaacg ccagcacgcc gaagacgatc gccgcgtaga cgaagaccgt catgggtctc
      361 aggggctccg ggcggtcaga cgcagcccgt gggcttgggc aggcccgcca ccttgcacgc
      421 gccattggcc gggccggacg ggaacagctc gtagacgcgg gcgagcttga gcccggtttc
      481 cttgcacagc ttccggacca tcggggcgat cccgaactgc aggtagtact cgcgcaggta
      541 ccgcacgagc ttccagtgct cgtcggtcat ctccgcgatc ccctcggtcg tcgcgaagtc
      601 ccgcgcaacg tcgtcgttcc agatctgcgg gtcctggagg aagccgttgt catcgacgtg
      661 gtagggcctg ccgttgagct cgacgatggg catacgcgtg ccgtcctgac tggtcccggt
      721 caacgggctt tcgccgcgcg cgcggcggca atctcgccga cggcgccgat ggccgcctcg
      781 atgtgcgcgt ccgtgttgaa cggcccgatc ccgaaccgga ccgcgccctt gatcgcgtcg
      841 aggcccagct gcgcgtgaac gagcggcgcg cagtgcagcc ccgtgcggca tgcgatgttg
      901 tgatccacgt ccaggatggt tccggtgttc cccgcctcga acccgtccac gttgaacacc
      961 atcacggcaa tgtggtcggc caagtcgtcc tgacagtaca tcgtgacgtt ggggatgccc
     1021 cggagcccct caaccagccg ccgcaggagc cgcatctcgt gcgcctcgat ggcggccagg
     1081 ccctgctgct cgagccagct gacgccggcg tggaggccgg cgatgcccag gacattgggc
     1141 gtgccgtact cgaggcgcca ggggtattcg tcgaggtgga agggatgcgc cgagcgtacg
     1201 cccgtgccgc ccgcgcgggt gtgccggatg tccacgtggg gctggacgta caggccgccg
     1261 atgcccgtcg gccccatcag cgacttgtgg cccgtaaacg ccagcacgtc cacgtgcatg
     1321 gcctgcacgt cgatggggat cttgcccgcc gtctgcgacg agtccacgac gaagcggatg
     1381 ccccgctcgc ggcacagcct gccgacctcg gcgacgggct ggacggtgcc gatgacgttc
     1441 gagccgtgat tcatcgcgac gacgcgcgtg ttcggcttga agcgggccgc gatgtcgccc
     1501 ggatcgacga agccccggcc gtcgaacggc acccgatcga cctcgacgcc gtggtactgc
     1561 gagaggtggt gcagcgggcg cagcaccgag ttgtgctcca ccgtcgtcgt gatggcgtga
     1621 tcgcccgggg acagcatccc gaacagggcc agattcaggg cgtcggtgga gttgtaggtg
     1681 aagacgagcc ggtcgggatc cgtgccgttg aagaagcggg tgagctgcct gcgg
//
LOCUS       DAOSIF010000607         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_60399_length_1718_cov_3.381840, whole genome shotgun sequence.
ACCESSION   DAOSIF010000607 DAOSIF010000000
VERSION     DAOSIF010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60399_length_1718_cov_3.381840"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLT35_14355"
     CDS             complement(<1..656)
                     /locus_tag="PLT35_14355"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOG30456.1"
                     /translation="MKRRTLGLLAGTGLVLVATAAALERGGGVLVDTAPVARKADFRS
                     TVTSSGQIVAQRYADIGSSVMGRLVQLRVREGDAVEAGQVVARIDAVQAAASASAANA
                     RVKASEAEVRGAADTLRAAEADLDAARARAQEAQLTLRRTRDLRADGLVPAAELDRAT
                     AAAETADAQLRGAEAALRRLQQAREATERRLAEARAEAARAADVLLRDDFVAGWHRRF
                     A"
     gene            796..>1718
                     /locus_tag="PLT35_14360"
     CDS             796..>1718
                     /locus_tag="PLT35_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG30457.1"
                     /translation="MPIRFRALVEAVIQGHSAISGHPLRAGLGALAVAVAVAAIVVVV
                     TALDGLTRYAHATGARAFGSETFLIAQIASAGGSTRRELAAKQQRNPPVGRPDLRFLE
                     RHASGIVYAPTVQRAGDVTAGPRKYENAAICGTTPSLAEIRDLGVARGRFLLPHEAER
                     GSQAAVIGADIAGTLFPGADAVGRSIRIAGRRFDVVGVQARLGTSGGTSLDRNVWIPL
                     AAFERVYGAAPTLSVFARAADGSNPEDVEAVAVAAMRAKRQLRPGVPDNFDLLTPEAA
                     RTFVRRLSERVGIASIPISAMALLAAVVVIT"
BASE COUNT          204 a          645 c          638 g          231 t
ORIGIN      
        1 gcgaaacggc ggtgccaacc ggcgacgaag tcgtcgcgca gcagcacgtc ggcggcgcgg
       61 gccgcctctg cgcgcgcttc ggcgaggcgg cgctcggtgg cttcccgggc ctgctggagg
      121 cgccggaggg cggcctcggc gccccggagc tgcgcatcgg ccgtttcggc ggcggccgtg
      181 gctcggtcaa gctcggcggc cggcacgagg ccgtcggcgc gcaggtcgcg cgtccgccgc
      241 agtgtcagct gggcctcctg ggcgcgggcc ctcgccgcgt caaggtcggc ttcggccgcc
      301 cgcagggtgt cggccgcgcc ccgcacctcg gcttccgagg ctttgacgcg cgcgttggcc
      361 gccgaggcgc tcgccgctgc ctgaacggcg tcgatgcgcg ccaccacctg gccggcctcc
      421 acggcatcgc cctctctcac gcggagctgg acgaggcggc ccatgacact ggagccgatg
      481 tcggcgtagc gctgcgcgac gatttggccc gacgaggtga ccgtcgatcg gaagtccgcc
      541 ttgcgcgcga ccggtgccgt atccaccagc acgccgccgc cgcgttcgag cgcggctgcc
      601 gtcgcgacga ggaccaggcc ggtgcccgcg agcagcccca gcgttcgccg cttcatacga
      661 cccactttac catgcgcgtc ggtccccggc cgcagagcca gcaccctggc gcggctgcgc
      721 cggacgccgg cgccttcgcc gcggcaaggg ctcctggcgc cggccgactt gcgctacagt
      781 acacggctcg gcgacatgcc catccggttt cgcgcgctcg tcgaggccgt cattcagggc
      841 cactccgcga tcagcgggca cccgctgcgc gcggggctcg gggccctcgc cgtggcggtg
      901 gccgtcgcgg ccatcgtcgt cgtggtcacg gccctcgacg ggttgacccg gtacgcccac
      961 gccaccggcg cgcgggcatt cggcagcgag acgttcctca tcgcgcagat cgccagcgcc
     1021 ggcgggagca cgcgccgcga actcgcggcc aagcagcagc gcaacccgcc cgtggggcgg
     1081 ccggacctca ggttcctcga gcgccacgca agcggcatcg tctatgcgcc gactgtgcag
     1141 cgcgcgggcg atgtcaccgc cgggccgcgg aagtacgaga acgcggcgat ctgcggcacg
     1201 acgccgtcgc tggccgagat ccgtgacctg ggcgtcgcgc gcggacggtt cctgctgccg
     1261 cacgaggccg agcggggcag ccaggccgcg gtgatcggcg ccgacatcgc cggcacgctc
     1321 tttccggggg cggatgccgt gggacggagc atccggattg ccgggcggcg gttcgacgtc
     1381 gtgggcgtgc aggcgcggct cggcacgtca ggcggcacgt cgctcgatcg gaacgtctgg
     1441 atcccgcttg ccgccttcga acgcgtgtac ggcgcggcgc cgacgctcag cgtcttcgcg
     1501 cgcgccgccg acggatcgaa tcccgaggac gtagaggccg tggcggtcgc ggcgatgcgc
     1561 gccaagcgcc agctcaggcc cggcgtgccg gacaatttcg acctgctgac gccggaagcg
     1621 gcccgcacct tcgtccggcg cctgtccgag cgcgtcggga tcgcctcgat ccccatctcc
     1681 gcgatggcgc tgctggcggc cgtcgtggtg atcacgaa
//
LOCUS       DAOSIF010000608         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_60453_length_1717_cov_4.277377, whole genome shotgun sequence.
ACCESSION   DAOSIF010000608 DAOSIF010000000
VERSION     DAOSIF010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60453_length_1717_cov_4.277377"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLT35_14365"
     CDS             complement(<1..217)
                     /locus_tag="PLT35_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOG30458.1"
                     /translation="MTKERIRAARDELRRHLTEELLPFWLARCKDDVNGGFVTHFDQH
                     GKDTGEDEKSLIAQSRCVYTFAAAHRAG"
     gene            complement(210..>1717)
                     /locus_tag="PLT35_14370"
     CDS             complement(210..>1717)
                     /locus_tag="PLT35_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG30459.1"
                     /translation="FNRRSPETDAAILVGLGRALDECDAVIVNQQVPGGLSDAFLDGL
                     NAALDAHPDRVVVFDSRHYGSRVRNIHRKTNEIEAARLSGVVLDPGVHPGLPDVEAFA
                     ARLFAESGKPVFITRGARGSVTVDGVGTHVAPGIQILGRVDPVGAGDTVTSALALCLG
                     AGVPPADAAAFANLAAAVTVRKLFQTGTASPEEVLAVCEDADYVYQPELAHDVRQAVF
                     VGGTELEACADPLPRLSSVRHAVFDHDGTISTLRQGWEQVMEPVMIRAILGERYPSAD
                     ETLYNRVRHRVLDYIDKSTGIETLVQMRALVGMVREFAVVPASEILDEAGYKAVYNDA
                     LMAVVGGRLAKYRRGELGLDDLTIKGAVEFLRALRDLGITLYLASGTDDADVKLEAEA
                     LGYADLFDGGIYGALGGGQTHSKRRVIQRILADHGLDGSCLAVFGDGPVELRESRRRQ
                     GAAIGIASDEVRRYGLDARKRARLIEAGAHIVMPDFSQASRLLALMRPDHD"
BASE COUNT          234 a          600 c          626 g          257 t
ORIGIN      
        1 agcccgcgcg gtgggctgcg gcgaaggtgt acacgcaccg gctctgcgcg atgagcgact
       61 tctcgtcttc tccggtgtcc ttcccgtgct ggtcgaagtg cgtgacgaag ccgccgttca
      121 cgtcgtcctt gcagcgcgcg agccagaagg ggagcagctc ctcggtgaga tggcggcgca
      181 gctcgtcccg ggccgcccgg attcgctcct tagtcatggt cgggcctcat gagcgccagc
      241 agccggctgg cctgcgagaa gtcgggcatg acgatgtggg cgcccgcctc gatgaggcgg
      301 gcgcgcttgc gcgcgtccag gccgtagcgg cgcacttcgt cgctggcgat gccgatggcg
      361 gcgccctggc gcctgcggga ctcccgcaac tcgaccggcc cgtcgccgaa cacggcgagg
      421 caggagccgt cgaggccgtg atccgccagg atgcgctgga tcacgcggcg cttcgagtgc
      481 gtctgcccgc cgccgagcgc cccgtagatg cctccgtcga acaggtccgc atagcccagg
      541 gcttccgctt cgagcttcac gtccgcgtcg tcggtgccgc tcgccaggta gagcgtgatc
      601 cccaggtccc ggagcgcacg caggaactcc acggcgccct tgatggtcag gtcgtccagg
      661 ccgagctcgc cgcgccggta cttcgcgagg cgcccgccga cgacggccat gagcgcgtcg
      721 ttgtagacag ccttgtagcc cgcctcgtcg aggatctccg acgccggcac gacggcgaac
      781 tcccgcacca tgccgaccag cgcccgcatc tggaccagcg tctcgatgcc cgtggacttg
      841 tcgatgtagt cgagcacgcg gtgccggacc cggttgtaga gggtctcgtc ggctgacggg
      901 tagcgctccc cgaggatcgc gcggatcatc acgggctcca tgacctgttc ccagccctgc
      961 cggagcgtgc tgatcgtgcc gtcgtggtcg aacaccgcgt ggcggacgga cgacaggcgc
     1021 ggcagcggat cggcgcacgc ctcgagctcc gtgccgccga cgaagacggc ctgcctgacg
     1081 tcgtgggcca gctcgggctg gtagacgtag tcggcgtcct cgcagacggc cagcacctcc
     1141 tcgggggagg cggtgccggt ctggaagagc ttgcgcacgg tgaccgccgc ggcgaggttg
     1201 gcgaaggcgg cggcgtcggc cggcgggacg cccgcgccca ggcagagcgc cagcgcgctc
     1261 gtgaccgtgt ctcccgcgcc gacggggtcg accctgccga ggatctggat gccgggcgcg
     1321 acgtgcgtgc ctacgccgtc caccgtcacc gagccgcgcg cgccgcgcgt gatgaagacc
     1381 ggcttgccag actcggcgaa cagcctggcg gcgaacgcct cgacatcggg gagaccgggg
     1441 tggacccccg gatcgaggac gacaccgctc agccgcgcgg cctcgatctc gttggtcttg
     1501 cggtggatgt tccggacgcg gcttccgtag tgccgggaat cgaacacgac gacccggtcc
     1561 gggtgcgcgt ccagcgccgc gttgaggccg tccaggaagg cgtcgctgag gcctccgggc
     1621 acctgctggt tgacgatgac cgcgtcgcac tcgtcgagcg cccgcccgag gccgacgagg
     1681 atggccgcgt cggtctcggg gctgcgccgg ttgaaga
//
LOCUS       DAOSIF010000609         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_60679_length_1713_cov_3.125452, whole genome shotgun sequence.
ACCESSION   DAOSIF010000609 DAOSIF010000000
VERSION     DAOSIF010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60679_length_1713_cov_3.125452"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLT35_14375"
     CDS             <1..635
                     /locus_tag="PLT35_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30460.1"
                     /translation="FGSADILLISYAYFCLQGEEGLTEATKHAILSANYVKSRLEAHY
                     PVLYTNARGRCAHELIFDLRAFKAAGIEEGDVAKRLMDYGFHAPTVSFPVPGTLMVEP
                     TESEPLDELDRFCDAMIAIRREIQDVLDGTSDRTDNVLKHAPHTMAVAAADVWTHPYS
                     REQAVFPLPFVKAGKVWPSVGRIDNPYGDRNLVCACPPPEAYSGSGGSTR"
     gene            complement(605..1540)
                     /locus_tag="PLT35_14380"
     CDS             complement(605..1540)
                     /locus_tag="PLT35_14380"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOG30461.1"
                     /translation="MLAVAYVNGRIVAPQDAMISVFDHGFLFGEGVYEVMRTYDGELF
                     LADRHFRRLRDSAAMISLEIGMPDQALARACRDTMDAFRTGPGRGAFDEELYVRLLVT
                     RGIGEMTYDPAKAYRPSVVVIVKPLGQPASWVYEQGVAVALVSVVRNHPRSISPRIKS
                     NNLLNNILAMQEALRRGPFAEAVMRNHRGELAECSQSNLFVVTGGAARTPALDAGLLA
                     GITREFVEEVASAAGIPVHDTRLTDEDLFGADEAFLTSTTKEIVPIVRVDDRPIGSGS
                     PGPVTRRLLEAYRVRARDVARAAASRYRVEPPLPE"
BASE COUNT          264 a          607 c          591 g          251 t
ORIGIN      
        1 ccttcggctc ggcggacatc ctgctcatct cgtatgccta cttctgcttg cagggagagg
       61 agggcctgac cgaagccacc aagcacgcca tcctgagcgc caactacgtc aagtcgcgcc
      121 tggaggcgca ctacccggtg ctctacacga acgcgcgcgg gcggtgcgcg cacgagctga
      181 tcttcgacct gcgcgcgttc aaggccgctg gcatcgagga aggcgacgtg gcgaagcggc
      241 tcatggacta cggtttccat gcgccgaccg tgtcgttccc ggtgccaggc accctgatgg
      301 tcgagccgac cgagagcgag cccctcgacg agctggaccg cttctgcgac gcgatgatcg
      361 ccattcgccg ggagatccag gacgtcctcg acggcacgag cgaccggacg gacaacgtcc
      421 tgaaacacgc gccgcacacg atggcggtgg cggccgccga cgtctggacc cacccgtact
      481 cgcgggagca ggcggtcttc ccgctgccgt tcgtgaaggc cggcaaggtc tggccgagcg
      541 tgggccggat cgacaacccc tacggcgatc gcaacctcgt ctgcgcctgc ccgccgcccg
      601 aggcctactc gggcagcggc ggctcgacgc ggtagcggct cgctgccgcc cgtgcgacgt
      661 cccgggccct gacgcggtac gcctcgagca ggcgccgcgt caccggcccg ggcgagccgc
      721 tgccaatcgg gcggtcgtca acgcgcacga tcgggacgat ctccttcgtc gtgctcgtca
      781 ggaacgcctc gtcggccccg aacaggtcct catccgtgag tctcgtgtcg tgtacgggga
      841 tgccggccgc cgaggcgacc tcttcgacga actcccgggt gatgcccgca agcaggccgg
      901 cgtccagcgc cggcgtacgc gccgcgccgc ccgtcaccac gaacaggttc gactgcgagc
      961 actcggccag ctcgccccgg tggttgcgca tgacggcctc ggcgaagggg ccgcgccgca
     1021 gggcctcctg catggcgagg atgttgttca gcaggttgtt cgacttgatc cgcgggctga
     1081 tcgagcgggg gtggttcctg acgaccgaca ccagcgcgac ggcgacgccc tgctcgtaga
     1141 cccaggaggc cggctggccc aggggcttga cgatgacaac gaccgacggg cggtaggcct
     1201 tcgccggatc gtaggtcatc tcgccgatcc cgcgcgtgac cagcagccgg acgtaaagct
     1261 cctcgtcgaa ggcgccccgc ccggggccgg ttcggaacgc gtccatggtg tcgcggcagg
     1321 cccgggccag cgcctggtcc ggcatcccga tctcgagcga gatcatcgcc gccgagtcgc
     1381 ggagcctccg gaagtgccga tcggcgagga agagctcgcc gtcgtacgtg cgcatcacct
     1441 cgtagacgcc ttcaccgaac aggaacccgt gatcgaacac cgagatcatc gcgtcctgcg
     1501 gtgcgacgat ccggccgttg acgtaggcca ccgcgagcat gcgctccgtc ctccttggcg
     1561 gccgtccccg gtgcgcgcgg caccggtctc cagacggccc cgcataaggt agctcctatg
     1621 gtagagtgcg cgcaagctgc gcgggcccgc gcgccggccg gcgaacgggc aggagggttg
     1681 cggtgcgggt gctgaacgcc gatcagatgc gcg
//
LOCUS       DAOSIF010000610         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_60748_length_1712_cov_2.432710, whole genome shotgun sequence.
ACCESSION   DAOSIF010000610 DAOSIF010000000
VERSION     DAOSIF010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60748_length_1712_cov_2.432710"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /gene="thpR"
                     /locus_tag="PLT35_14385"
     CDS             <1..419
                     /gene="thpR"
                     /locus_tag="PLT35_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOG30462.1"
                     /translation="TVLAPPLAAGPLRVELGDWGVFPTAGPARVIWIGLSAGAAALAR
                     LHAELAGRLRQAGIALDDRPFSPHLTIGRVKQPGRADWAALVRSVAPPACGFDVVACT
                     LYRSHLAGAGPSYEPLLRISLEARPAGVPGAGDRLA"
     gene            419..1369
                     /gene="ftsY"
                     /locus_tag="PLT35_14390"
     CDS             419..1369
                     /gene="ftsY"
                     /locus_tag="PLT35_14390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOG30463.1"
                     /translation="MGLFGSVREGLRRTARQLLGRFDEISRRADAPERRSAPVDAETA
                     EALEELLIGADMGVAAAGRTVAAVTGRARRGEPLRDLVKAELLSILQGASRTAPPVGD
                     PTVIMLVGVNGTGKTTSVAKLAYLHRAQGARPLVCAADTFRAAAVEQLEIWARRAEVE
                     IIRGREGSDPASVVFDALAAGKARGWSPVIVDTAGRLHTRANLMQELAKIRRIAEREV
                     PGAPHEVLLVLDATVGQNALVQAREFMAAAGVTGIVLAKLDGTAKGGIALAIAHDLGL
                     PIRYVGVGEGIGDLVPFSAPEYVDALFENSWEGGRPASQR"
     gene            1495..>1712
                     /locus_tag="PLT35_14395"
     CDS             1495..>1712
                     /locus_tag="PLT35_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOG30464.1"
                     /translation="MFESLSERLQGIFRTLRGEARLSESAVESALREIRMALLEADVN
                     FRVTKAFVDRVRERAVGQDVLRSLTPGQ"
BASE COUNT          218 a          615 c          633 g          246 t
ORIGIN      
        1 agaccgtgct cgcgccgcct cttgcggcgg ggcctttgcg cgtcgagctg ggcgactggg
       61 gcgtctttcc gacggccggc ccggcccggg tcatctggat tggcctctcg gcgggcgcgg
      121 cggcgctcgc gcgcctccac gccgagctcg cggggcgcct tcgccaggcc ggcatcgccc
      181 tcgacgacag gcccttttcg ccccatctga ccatcggccg cgtgaagcag cccggccgag
      241 ccgactgggc cgctctggtc cggagcgtgg cgccgccggc gtgcgggttc gacgtcgtgg
      301 cctgcacgct ctaccggagc cacctcgccg gcgcggggcc gtcctacgag ccgctgctgc
      361 gcatttccct ggaggcgcgg cccgcaggcg ttcccggagc gggtgataga ctggcgtgat
      421 gggactgttc ggcagcgtgc gtgaagggct gcgccgcacg gcgcggcagc tgctggggcg
      481 tttcgacgag atctcgcggc gcgcggacgc ccccgagcgc cgctcggcgc ccgtcgatgc
      541 cgagaccgcg gaggccctgg aagagctgct catcggcgcc gacatgggcg tggctgccgc
      601 cggccgcacc gtcgcggcgg tgaccggccg cgcccgccgc ggcgagccgc tgcgggatct
      661 ggtcaaggcc gaactcctgt ccattctcca aggcgcctcc cggaccgcgc cgcccgttgg
      721 cgatccgacg gtcatcatgc tcgtcggcgt caacgggacg ggcaagacga ccagcgtcgc
      781 gaagctcgcg tacctgcacc gcgcgcaggg cgcccgcccg cttgtgtgtg ccgccgacac
      841 cttccgcgcg gcggcggtcg agcagctcga gatctgggcc aggcgggccg aggtggagat
      901 catccgcggg cgcgagggat cggacccggc ctccgtcgtc ttcgacgcgc tggctgccgg
      961 caaggcgcgc ggctggagcc cggtcatcgt ggacaccgcc ggccggctgc acacccgcgc
     1021 gaatctgatg caggagctgg cgaagatccg ccgcatcgcc gagagggaag tgcccggcgc
     1081 cccgcacgag gtgttgctcg tgctcgacgc gacggtcggg cagaacgcac tcgtccaggc
     1141 ccgcgaattc atggcggcgg ccggcgtgac cggcatcgtc ctcgccaagc tggacggcac
     1201 ggcgaaaggc ggcatcgcgc ttgccatcgc gcacgatctc ggcctgccca ttcgctacgt
     1261 cggggtcggc gagggcatcg gcgatctcgt gcccttctcg gcgcccgagt acgtggacgc
     1321 gctgtttgag aacagctggg aaggcggccg gcccgcttcc cagcgttgac agtcccgctc
     1381 aaccgacgct acgataaggg tttgcgccat ctcgcgaggc gcttgtctga ccgcgtcagc
     1441 cgcctcgagc gggacggctg gcagcaggcg cccggcgcga gcgcgactgc accgatgttc
     1501 gagtcactga gcgaacggct ccaggggatc ttccgcacgc tgcgcggcga ggcgcggctg
     1561 tcggaatcag ccgtggagtc ggcgctgcgc gagatccgca tggcgctgct cgaggccgac
     1621 gtcaacttcc gggtgaccaa ggccttcgtc gatcgcgtgc gcgagcgggc cgtaggccag
     1681 gacgtcctgc gaagcctcac cccggggcag ca
//
LOCUS       DAOSIF010000611         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_61152_length_1704_cov_2.314736, whole genome shotgun sequence.
ACCESSION   DAOSIF010000611 DAOSIF010000000
VERSION     DAOSIF010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61152_length_1704_cov_2.314736"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLT35_14400"
     CDS             <1..223
                     /locus_tag="PLT35_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG30465.1"
                     /translation="VPVLAAIGLLLAAANFLFIIATGLGYLSVISILGSLSPVVVTAY
                     AQLLLHERLAPRQWAAFAAVFVGIALLSL"
     assembly_gap    343..352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <353..1527
                     /locus_tag="PLT35_14405"
     CDS             <353..1527
                     /locus_tag="PLT35_14405"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF005492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOG30466.1"
                     /translation="NPHQTPAGVTVPEEAGLRVLSGRPGYINFLDALGAWQLVRELKE
                     ATGKPAAASFKHVSPAGAAVAGELSAPYRASQFLGDEEYSPLATAYVRARGGDRMCSF
                     GDVAAVSDVVDLSLARMLFDEVSDLIIAPGFEPAALELLTAKRDGAYMILAVDPDYEP
                     PDVESRQVFGFTMTQKRNTARITPGLFRAAGSAPDAAARGAAAGTAPLPDDVLETLIV
                     ATVALKYTQSNSVGVACNGQVIGMGAGQQSRVHCTRLACDKADKWLLQQHPKTLALPI
                     RQELPKPEKANLVDYYLLWDGLSDVERRKLREGLTADPDPLTREEREEWVKRFDGICL
                     SSDAFIPFPDNLDRAARSNVQYVAQTGGSARDAVVTEAAAEYGMTMVHTGLRLFLH"
BASE COUNT          243 a          620 c          585 g          246 t
ORIGIN      
        1 cgtgcccgtg ctcgccgcga tcggccttct gctcgcggcc gccaacttcc tgttcatcat
       61 cgccaccggg ctgggctacc tgagcgtgat cagcatcctc ggctcgctct cgccggtcgt
      121 ggtgacagcc tacgcccagc tgctgctgca cgaacggctc gccccgcgcc agtgggccgc
      181 cttcgcggcg gtgttcgtcg ggatcgcgtt gctctcactc tgagccgcgt cccccggcga
      241 agtggcgtcc gccggacagg tccttctcct gctggacggg ccgggcggac ggcgtagact
      301 gatccggcgc ctgtgacacc cgccggaaag gaagcccgat ggnnnnnnnn nngcaacccg
      361 catcagacgc ccgcgggggt cacggtcccg gaggaggccg gtctccgcgt gctgagcgga
      421 cggccgggct acatcaactt cctcgacgcg ctcggcgcct ggcagctggt gcgcgagctg
      481 aaggaggcga ccgggaagcc ggcggccgcg tccttcaagc acgtgagccc ggccggcgcc
      541 gccgtcgccg gcgagctgtc cgcgccgtat cgcgcctccc agttcctcgg cgacgaggag
      601 tactcgccgc tggcgacggc ctacgtgcgc gcccgcggcg gcgaccgcat gtgctcgttc
      661 ggtgacgtcg ccgccgtgag cgacgtggtc gacctctcgc tggcgcggat gctcttcgac
      721 gaggtgtccg acctcatcat cgcccccggg ttcgaaccgg cggcgctcga gctcctgacg
      781 gccaaacgcg acggcgccta catgatcctc gccgtcgacc cggactacga gccgcccgac
      841 gtggagtcgc gccaggtctt cggcttcacc atgacccaga agcgcaacac cgcccggatc
      901 accccgggac tgttccgggc cgccggcagc gcgcccgacg cggccgcccg cggcgcggcc
      961 gctggaaccg cgcccctgcc ggacgacgtg ctcgagaccc tgatcgtggc gacggtggcc
     1021 ctcaagtaca cgcagtccaa ctcggtcggc gtcgcctgca acgggcaggt gatcggtatg
     1081 ggcgcaggcc agcagtcccg cgtgcactgc acgcgtctgg cctgcgacaa ggccgacaag
     1141 tggctgctgc agcagcatcc caagacgctc gcgctgccga tccggcagga gctccccaag
     1201 cccgagaagg ccaacctcgt cgactactac ctgctctggg acggactctc cgacgtggag
     1261 aggcgcaagc tgcgcgaggg actgacggcg gacccggacc cgctcacgcg cgaggagcgc
     1321 gaggaatggg tcaagcgctt cgacggcatc tgcctgagct ccgacgcctt catcccgttc
     1381 cccgacaacc tcgaccgcgc cgccaggagc aacgtgcagt acgtcgccca gactgggggt
     1441 tcggcgcgcg acgccgtggt cacggaggcg gcagcggagt acgggatgac gatggtgcac
     1501 accgggctgc gcctgttcct gcactgaggg acgtcctcgc gcgagcggaa cgtcccggca
     1561 gccggggcgc ctcgatcgtg aactcgagcg cggtcgtcaa cgacgtcgag ccgttcttcg
     1621 cccggcatgc cggagtgcgc gccgtcttct tcaacggccg cacggcgcgc gggctccggg
     1681 atcgtcgcgt ggaagggagt caag
//
LOCUS       DAOSIF010000612         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_61401_length_1699_cov_3.994526, whole genome shotgun sequence.
ACCESSION   DAOSIF010000612 DAOSIF010000000
VERSION     DAOSIF010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61401_length_1699_cov_3.994526"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..99)
                     /locus_tag="PLT35_14410"
     tRNA            complement(25..99)
                     /locus_tag="PLT35_14410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65..67),aa:Val,seq:tac)
     gene            complement(144..704)
                     /locus_tag="PLT35_14415"
     CDS             complement(144..704)
                     /locus_tag="PLT35_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30467.1"
                     /translation="MAVVRSLLLRAVRVLLSVMYLLGSRTVVAAGLTLAVWTGAGAAR
                     NLWFAFSGTAVEGIVVRQIEEYSADWNETQRVPADARAAGIDLAAAQRRCRAVVQFRQ
                     SGRIFEVVSSLADASPRYPLHSTVVAVYPPGRPERARLRPELPDGWVQAGLLFAATML
                     GAGSVRLWWALARRRAGRRRVVTPVG"
     gene            923..>1699
                     /locus_tag="PLT35_14420"
     CDS             923..>1699
                     /locus_tag="PLT35_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG30468.1"
                     /translation="MRQGFAQDVPQNVPPPAGVHRQARLRLGLALGGGGARGFAHLGI
                     LIWLDEHRIPVDVVGGTSMGALIGGAFAAGMTPGEIREIVASIDWAAVLAPETPYASM
                     TFRRREDRRDYPSQLRFGLKAGLKLPTGVSPAQPIELLLARLAAPFAGDVDFDALPTP
                     FRCVSADIMNAEAVVFSGGSLATALRATMAMPGLFAPVETDGRRLVDGGLVNNLPADV
                     VRRTGLADRVVAVDVGADLAIARRGDTMFDVLGQALDSIMR"
BASE COUNT          238 a          633 c          599 g          229 t
ORIGIN      
        1 cggccgaacc ccgtggcagc ccggtggtgg gcggtacagg attcgaacct gtgacagccg
       61 gcgtgtaaag ccggtgctct accgctgagc taaccgcccc gacacgacga tccggccgcc
      121 tcgcaaactc cgcattctac catctacccg accggcgtga caacgcgcct ccgcccggcg
      181 cgccttcgcg ccagagccca ccacagccgg acgctcccgg ccccgagcat cgtcgccgca
      241 aacagcaggc cggcctgcac ccagccgtcc ggcagctcgg gcctgagcct ggcccgttcg
      301 gggcggcccg gcgggtagac ggcgacgacg gtcgagtgca acgggtagcg cggcgacgcg
      361 tcggccagac tggacacgac ctcgaagatc ctgccgctct ggcggaattg caccacggcg
      421 cggcagcggc gctgagcggc ggcgaggtcg atccccgcgg cgcgggcgtc cgccggcacc
      481 cgctgcgtct cgttccagtc cgccgagtac tcctcgatct gccggacgac gatgccttcg
      541 acggcggtgc cgctgaaggc gaaccacagg ttccgcgccg cgccagcgcc ggtccacacc
      601 gccagcgtca ggccggcggc gacaaccgtg cgcgacccca gcaggtacat caccgacagg
      661 agcacgcgca ccgcacgcaa gagaaggctc cggaccacgg ccacgattgt atccgcttca
      721 cagtacacta gtcgcgggac tctcactggg ccggcgcggc cgtccccggc gcgcgggcgc
      781 gcgcctgcgt gcccgagatc cggtgcatga ccaacccagg cggacgccgg ctcctggatg
      841 gaggcgtcgg cgcacgttgc ggcggccatc gacacgggcg gtcggggcgg gggttctcgc
      901 tgtcatcctg ctcgcaggag cgttgaggca ggggttcgcc caggacgtcc cccagaacgt
      961 gccgccccct gccggcgtac atcggcaggc gcggctccgc ctgggactcg ccctcggcgg
     1021 cggcggcgcc cggggcttcg cgcacctcgg cattctgatc tggctcgacg agcaccgcat
     1081 cccggtcgac gtggtcggcg ggaccagcat gggcgcgctc atcggcggcg cgttcgccgc
     1141 gggcatgacg cctggcgaga tccgcgagat cgtcgcgtcg atcgactggg cagcggtgct
     1201 ggcgccggag acaccctacg cgtcgatgac gttccgccgc cgggaagaca ggcgcgacta
     1261 cccgtcgcag ctgcgcttcg gcctgaaggc cggcctcaag ctgccgacgg gcgtcagccc
     1321 cgcgcagccg atcgagctcc tcctcgcccg cctcgcggcc ccgtttgcgg gagacgttga
     1381 tttcgacgcg ctcccgactc cgttccgctg cgtctcggcc gacatcatga acgccgaggc
     1441 ggtcgtcttc agcggcggct cgctggccac cgccctgcgc gcgacgatgg cgatgcccgg
     1501 cttgttcgcg ccggtggaga ccgacggccg ccggctcgtc gacggcggac tggtgaacaa
     1561 ccttccggcg gacgtcgtcc gccggaccgg gctggccgat cgcgtcgtgg cggtggacgt
     1621 gggagccgat ctggccatcg cgcggcgcgg ggacacgatg ttcgacgtgc tcggtcaggc
     1681 gctggacagc atcatgcgg
//
LOCUS       DAOSIF010000613         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_61642_length_1695_cov_5.510366, whole genome shotgun sequence.
ACCESSION   DAOSIF010000613 DAOSIF010000000
VERSION     DAOSIF010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61642_length_1695_cov_5.510366"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="PLT35_14425"
     CDS             complement(<1..1645)
                     /locus_tag="PLT35_14425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG30469.1"
                     /translation="MAALLLLITGFVVCFYHLGKADFYRDAPARVAANVRQMNLEGVW
                     IPPRAGDPGRVELPPAYPWAVRLASGLRGSVSAFQTRLPSAISGLLLLFLAALWFYGH
                     AVRYTRDDGADAPAEGFSLLAGLMLAASPIFFICARAGTPEALFTLLWAAAAFCWTES
                     LEARRSFYAGRPWRVWIFWGYGLAGLAILVHGLLILPMLWLPYLLAARSYRLRGPDWI
                     HLAGLLLALCIGGAWYVFLARLDPHRAGDLARELLTLRLGREAMPRLGIGHYGLALLV
                     GTFPWLLLALVMAVRVQRKLSRSPTLVMWLWSLVVNLVLLSVLAPHAGAFRLAPALLI
                     VLLAASGLQRWNFENRWAVALRAGLRILIILLIAVGTFLALLLNSTEGLLFFIVIALG
                     WLGWTIYSRRQGIIYSPWNATVRLAAMAVLILLAGETLHLTNWEPRKRYHVDTIAYFN
                     RVQDALRGLRPKAFAVELGAAERKESKTRLFFWGDRTAGLHDFYLDGRATPAHDLAAL
                     YARAGESTYLFAEHDLARLLSEPRLVPTVTQPGRRPELPA"
BASE COUNT          318 a          528 c          667 g          182 t
ORIGIN      
        1 gggctggcag ctcgggacgg cggccgggct gggtgacggt gggcaccagc cgcggttcgc
       61 tgagcaggcg cgccagatca tgctcggcga agaggtaggt gctttcgccg gcccgcgcgt
      121 aaagcgccgc caggtcgtgg gccggggtcg cccggccgtc gaggtaaaaa tcatgcaggc
      181 ccgcggtgcg gtcgccccag aagaagaggc gggttttcga ttccttgcgc tcggcggcgc
      241 ccagctcgac ggcgaacgcc ttcggccgga gtccgcgcag cgcatcctgc acccgattga
      301 agtaggcaat cgtatccaca tggtagcgct tgcgcggctc ccagttggtc aggtgcagcg
      361 tctcgccggc gagcaggatc aggacggcca tcgcggcgag gcgaaccgtc gcgttccagg
      421 gcgaatagat gatcccctgg cggcgggaat agatcgtcca gccgagccag cccagggcga
      481 tgacgatgaa gaagagcaag ccctcggtgg aattgaggag cagggcgagg aatgtgccga
      541 cggcgatcag caggatgatg aggatgcgca gccccgcgcg cagcgcgacg gcccagcggt
      601 tctcgaaatt ccagcgctgc aggccgctgg ccgcgagcaa cacgatcagc agcgcggggg
      661 cgagccggaa cgcgccggca tgcggcgcga ggaccgacag gagcaccagg ttgacgacga
      721 gcgaccagag ccacatcacg agcgtcggcg agcggctcag cttgcgctgg acgcggaccg
      781 ccatgacgag cgccagcaga agccagggga acgtcccgac cagcagcgcg agcccgtaat
      841 gcccgatgcc gaggcggggc atggcctcgc gcccgaggcg gagcgtcaac agctcgcgcg
      901 ccagatcgcc cgcccggtgg gggtccaggc gcgcgaggaa tacataccat gcgccgccga
      961 tgcagagcgc gaggaggagg ccggccagat ggatccagtc cgggccgcgc aggcgatagg
     1021 agcgcgccgc aagcaggtag ggaagccaca gcatcggcag gatgagcagg ccgtggacga
     1081 ggatcgcgag gccggccagg ccgtagcccc agaagatcca gacgcgccag ggccggccgg
     1141 catagaacga gcggcgggcc tcgagcgatt cggtccagca gaaggcggcg gcggcccaga
     1201 gcagcgtgaa cagggcctcg ggggtgccgg cgcgggcgca gatgaagaag atcggcgagg
     1261 cggcgagcat caggccggcc agcagcgaaa agccctcggc gggggcgtcg gcgccgtcgt
     1321 cgcgcgtgta gcgcacggca tggccgtaga accagagcgc ggccaggaag agcaggagca
     1381 ggccgctgat cgccgacggc aggcgggtct ggaacgccga gacactcccc cgcaggccgg
     1441 aggcaaggcg cacggcccag ggataggccg gggggagttc gacgcggccg gggtcgccgg
     1501 cgcgcggcgg gatccagacg ccctcgaggt tcatctggcg gacgttcgcc gcgacgcggg
     1561 cgggggcgtc ccggtagaag tcggctttgc cgaggtggta gaagcagacg acgaagccgg
     1621 tgatcagcag cagcagggcc gccatcagtg ttccgagcga aaacggccct tcctgcgtgc
     1681 gcattgggga gcggc
//
LOCUS       DAOSIF010000614         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_62033_length_1688_cov_2.633190, whole genome shotgun sequence.
ACCESSION   DAOSIF010000614 DAOSIF010000000
VERSION     DAOSIF010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62033_length_1688_cov_2.633190"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1064
                     /gene="kdsB"
                     /locus_tag="PLT35_14430"
     CDS             279..1064
                     /gene="kdsB"
                     /locus_tag="PLT35_14430"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOG30470.1"
                     /translation="MHPPAGVEAGRSASAPRVAVIIPSRYAATRLPGKPLCDIGGRPM
                     IEHVYRRAAAARGVEAVLVATDDERIAAAVDAFGGRSVMTDPGHASGTDRIAEVARGL
                     SCDLVVNVQGDEPALAPSAIEDAVAPLASDASIVMGTLGGPLDPDADAGNPNVVKVLV
                     DRQGFALYFSRAPIPYPRNPEAPTGAALRHVGLYVYRRDFLLTLASLPRTPLERIESL
                     EQLRALEHGYRIRVVASAYRPSSVDTPEDLERVRRMAADGLLP"
     gene            1061..>1688
                     /locus_tag="PLT35_14435"
     CDS             1061..>1688
                     /locus_tag="PLT35_14435"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOG30471.1"
                     /translation="MNARMDRPGQNRVKYIFVTGGVASSLGKGLAAASIGSLLEGHGY
                     RVTLQKLDPYINVDPGTLSPYQHGEVYVTDDGAETDLDLGHYERFTNARMTRNHNWTA
                     GRVYLSVIQKERRGDYLGRTVQVIPHITDEIKQCIRSVAVGVHVVIVEIGGTVGDIES
                     QPFLEAVRQLRQDLGRESTLFVHLTLVPFIGTAGELKTKPTRHSVRDLR"
BASE COUNT          254 a          634 c          571 g          229 t
ORIGIN      
        1 gccttcaggc gcgcccgccc gcccgcccgc cgccgcggcc gcccacacag ccacgaggtt
       61 cacaccgacc acgacgaccg ctgcacgcga aggagtcatg ccgtcacctg cgacagtgac
      121 ggtattctac cccacgcgcg cgagcccgct gcaccgggcc tcgcgccgcg cgccgagcgc
      181 gcgatcgaat catggccggc ccgctcgagg catccccggc tggcaggtgc gccgcgcacg
      241 gcagccaggg caccccggcg cgtgtgctat gattcgccgt gcaccctcca gcaggcgtcg
      301 aggccggccg ctcggcatcc gcccctcgcg tggccgtcat catcccttcc cgctacgcag
      361 cgaccaggct gccgggcaag ccgctgtgcg atatcggcgg acggccgatg atcgagcacg
      421 tgtaccgccg cgccgccgcc gcgcgcggcg tggaggccgt tctcgtcgcg accgacgacg
      481 agcggatcgc ggcggcggtg gacgccttcg gcggccgctc ggtgatgacg gatccgggcc
      541 acgccagcgg caccgaccgg atcgccgaag tggccagggg cctctcctgc gacctcgtcg
      601 tgaacgtcca gggcgacgag ccggccctcg cgccgtcggc catcgaggat gcggtggcgc
      661 ccctcgcctc cgacgcctcg atcgtcatgg gcacgctcgg cggcccgctc gatcccgacg
      721 ccgacgccgg gaatcccaac gtcgtgaagg tgctggtcga ccgtcagggg ttcgccctgt
      781 acttctcccg cgctcccatt ccctacccgc ggaacccgga agccccgacc ggcgcggcgc
      841 tcaggcacgt ggggctgtac gtctaccggc gcgacttcct cctgacgctc gcgtcgctcc
      901 cgcgcacgcc gctcgagcgc atcgagtcgc tggaacagct gcgcgcgctg gagcacggat
      961 accggatccg ggtggtggca tcggcctacc ggccgtcgtc ggtcgacacg ccggaggacc
     1021 tcgagcgcgt ccgccggatg gcggcggatg gtctgctccc gtgaacgcgc gcatggatcg
     1081 gcccggccag aaccgcgtca agtacatctt cgtcacgggc ggcgtggcct cgtcgctggg
     1141 caaggggctg gccgccgcct cgattggaag ccttctcgag gggcacggct accgcgtgac
     1201 gctgcagaag ctggatccct acatcaacgt ggacccgggc acgctgagcc cgtaccagca
     1261 cggcgaggtc tacgtgaccg acgacggcgc ggaaacggac ctcgacctgg gccactacga
     1321 gcggttcacg aacgcgcgga tgacgcgcaa ccacaactgg accgccggcc gcgtctacct
     1381 ctcggtgatc cagaaggagc ggcgcggaga ctacctgggg cgcacggtcc aggtgatccc
     1441 gcacatcacc gacgagatca agcagtgcat ccgatccgtc gccgtcggcg tccacgtggt
     1501 aatcgtcgag attggcggga cggtcggcga catcgagagc cagccgttcc tcgaggcggt
     1561 gcgccagctt cgccaggacc tagggcgcga gagcacgctc ttcgtccacc tgacgctggt
     1621 cccgttcatc ggcaccgcag gcgagctgaa gacgaagccg acgcggcaca gcgtccgcga
     1681 ccttcgct
//
LOCUS       DAOSIF010000615         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_62339_length_1682_cov_3.253227, whole genome shotgun sequence.
ACCESSION   DAOSIF010000615 DAOSIF010000000
VERSION     DAOSIF010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62339_length_1682_cov_3.253227"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /gene="rpoN"
                     /locus_tag="PLT35_14440"
     CDS             <1..1385
                     /gene="rpoN"
                     /locus_tag="PLT35_14440"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOG30472.1"
                     /translation="LILTPSLQQAIKLLPMSTLELADMLNQEVVENPMLEEVQPEDAQ
                     AADAAAAEKTEADREAAKADGWDEGDYAYFFGDYLDEGYRPRAPQEVRELPPIENTLS
                     TTSTLTDHLEWQLSLQAADDEVRAIGEAIIGNLDDDGYLVASVDEIAAMGDWSADRVE
                     STLQMIQGFDPIGVAARDLQECLWLQLRHIGFEGSPAEKIVTEHLRMLQNHQVPELAK
                     KLGLSVEDVKEHIETIRHLDPKPGSRFNRRESQYVIPDVYVVKVEDQYVAVLNEDGLP
                     QLRISPVYRRLLEKNAEHPEETRAYVKDKFRSALWLIKSVEQRQRTIHKVATSIIHFQ
                     KDFLDHGIEFLRPLVLRDVASDISMHESTVSRVVTNKYMHTPQGVFEMKYFFHSGISS
                     SYGDQVSSVAIKQRIKKIIDGEDRRKPLSDSKIVSILQKEGLVLARRTIAKYREELKI
                     PTSNQRKTFY"
     gene            1393..>1682
                     /gene="raiA"
                     /locus_tag="PLT35_14445"
     CDS             1393..>1682
                     /gene="raiA"
                     /locus_tag="PLT35_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG30473.1"
                     /translation="MRLVLTGRQVEVTPDIRRMVEKKAARLARVLGARAVSGQVELRL
                     EKYRHVVELHVHVRGGHILKGRAVATSWDEAAALAVDRLVQQGQRLKGKWQER"
BASE COUNT          328 a          521 c          597 g          236 t
ORIGIN      
        1 agctgatcct gacgccttcg ctccagcagg cgatcaagct cctgccgatg tcgacgctgg
       61 agttggcgga catgctcaac caggaggtcg tcgagaaccc gatgctggag gaggtgcagc
      121 cggaggatgc gcaggcggcc gacgcggcgg cggccgagaa gaccgaggcc gatcgggaag
      181 cggccaaggc ggacggctgg gacgagggcg actacgccta cttcttcggc gactacctcg
      241 acgagggcta ccggccgagg gcgccgcagg aggtccggga gctgccgccc atcgagaaca
      301 cgctgtcgac gacgtccacg ctcaccgatc accttgagtg gcagctgtcg ctgcaggccg
      361 ccgacgacga ggttcgcgcg atcggcgagg ccatcatcgg caacctggac gacgacggct
      421 acctcgtggc gtcagtcgac gagatcgcgg cgatggggga ctggagcgcg gaccgcgtcg
      481 agtccacgct gcagatgatc cagggcttcg acccgatcgg cgtggcggcg cgcgacctgc
      541 aggagtgcct ctggctgcag ctgcggcaca tcggcttcga aggctcgccg gccgagaaga
      601 tcgtgaccga gcacctgcgc atgctccaga accaccaggt gccggagctg gcgaagaagc
      661 ttggcctgtc ggtcgaggac gtgaaggagc acatcgagac gatccggcac ctggatccga
      721 agcccggcag ccggttcaac cggcgggagt cccagtacgt cattcccgac gtgtacgtgg
      781 tcaaggtcga ggaccagtac gtggccgtgc tgaacgagga cgggctgccg cagctgcgga
      841 tcagcccggt ctaccgccgg ttgctggaga agaacgccga gcacccggag gagacgcggg
      901 cctacgtcaa ggacaagttc cgctcggcgc tctggctgat caagtccgtg gagcagcgcc
      961 agcggaccat tcacaaggtc gcgacgagca tcatccactt ccagaaggac ttcctcgacc
     1021 acggcatcga gttcctgagg ccgctggtcc tgcgcgacgt ggccagcgac atcagcatgc
     1081 acgagtccac ggtgagccgc gtggtgacga acaagtacat gcacaccccg cagggcgtgt
     1141 tcgagatgaa gtacttcttc cacagcggca tcagcagctc gtacggcgat caggtgtcgt
     1201 cggtggcaat caagcagcgc atcaagaaga tcatcgacgg cgaggaccgg cggaagccgc
     1261 ttagcgactc gaagatcgtc agcatcctgc agaaggaggg cctggtgctg gcgcggcgga
     1321 cgatcgcgaa gtaccgcgaa gagctgaaga ttccgacgtc caaccagcgg aagacgttct
     1381 actgaggcca ccatgcgact cgtgctcacg ggcaggcagg tcgaagtcac gccggacatc
     1441 cgccggatgg tggagaagaa ggcggcgcgt ctggcgcgcg tgctgggcgc gcgcgccgta
     1501 tcgggccagg tcgagttgcg cctcgagaag taccgccacg tcgtcgagct gcacgtgcac
     1561 gtgcgcggcg gccacatcct caaagggcgc gccgtggcga cctcctggga cgaggcggcc
     1621 gcactggccg tcgatcgcct cgtgcagcag ggccagcggc tgaaggggaa gtggcaggag
     1681 cg
//
LOCUS       DAOSIF010000616         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_62542_length_1678_cov_3.452249, whole genome shotgun sequence.
ACCESSION   DAOSIF010000616 DAOSIF010000000
VERSION     DAOSIF010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62542_length_1678_cov_3.452249"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLT35_14450"
     CDS             complement(<1..548)
                     /locus_tag="PLT35_14450"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG30474.1"
                     /translation="MFDFANSSYTTVIVTVVFSVVFPRLVVGDGPEYRYGNLLWSVAL
                     SVSYALVVLTAPLLGAIMDFRAAKKRFLFGSYALTVVATAMLYFVTPGRAPLGVALVV
                     LSNYGFSVGESFTSSFLPELGPVDELGKISGYAWGLGYIGGVVCTALVIGIVGDQTAA
                     NFERVRLVGPVTALFFLVAAIPT"
     gene            1210..>1678
                     /locus_tag="PLT35_14455"
     CDS             1210..>1678
                     /locus_tag="PLT35_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOG30475.1"
                     /translation="MSDHPEFTKATLDQWLKAAAKVAPGGKVEALNWVTPDGLSVKPL
                     YTAADTNGLPYTDTLPGFEPFVRGPQPTMYAGRPWTIRQYAGFSTAEESNAFYRQSLA
                     GGQQGISVAFDLATHRGYDSDHPRVTGDVGKAGVAVDSVEDMKILFDGIPLDKV"
BASE COUNT          274 a          606 c          602 g          196 t
ORIGIN      
        1 gtggggatgg ccgccaccag gaagaacagc gccgtcaccg gcccgacgag gcgcacacgc
       61 tcgaagttcg ctgccgtctg atcgcccacg atcccgatga cgagagccgt gcagacgacg
      121 ccgccgatgt agcccagtcc ccaggcgtac cccgagatct tgcccagctc gtcgaccggc
      181 ccgagttcag gcaggaagct cgacgtgaag gactcgccga ccgagaagcc gtagttcgac
      241 agcacgacga gcgccacgcc cagcggcgcg cggccgggcg tcacgaagta cagcatcgcc
      301 gtcgcgacca cggtgagcgc atagctgccg aacaggaacc gtttcttcgc cgcgcggaag
      361 tccatgatcg cgccgagaag cggcgccgtt agcacgacca acgcgtagct caccgacagc
      421 gccacgctcc acagcaggtt cccgtagcgg tactccgggc cgtcgcccac caccagccgc
      481 gggaagacca cgctgaagac gacggtgacg atcaccgtcg tgtagctgga attggcgaag
      541 tcgaacatcg cccagccgaa gatctcacgg gccggcgcgc gcggcgtcgt cggcgggggc
      601 gggggtgcgg ccgccggcgc cgctggaggg ccgggcgagg cagggaccgt ggaggatgcg
      661 gccatggcgt gagcgcgagg gtaacacgga gggggcggga ggagggaacg caggcggagc
      721 ggccagaagt cggaactccg cagccggggc agccctggcc tcaggagcgt cgatggcggc
      781 tccggcgcgc tgattcttct tcgcgggctc gccggtccgg ccgcccagtc gctgccgccg
      841 cgaccgcacc ggccgcgcgg ttcccgcgcg cgtgcgcgtc cacgaggtga ccggctcacc
      901 ggggccgaga atcgtctgcc gccgcccggg tgagcgacaa cggcgcaagg ccggcagacg
      961 ccgacgcgcg cgacgccggg cagcgccgca ggaccgaggg cgagcgctgg ccgcccgccg
     1021 tcagccgctt caccggcagc gcccgcgttt tcttcgtctg gcgcctcgac gtgggtcagg
     1081 cctcggggtc cccggctacc ggcggaacga cggcccctga ccccggctgc gctatgatcg
     1141 gatggccgcc gattctcggc ggctgagccc ccccagagcg ggccgccggc ccgcgcgcag
     1201 gagactccgg tgtccgatca tcccgagttc accaaggcga ccctcgacca gtggctgaag
     1261 gcggcggcga aagtcgcgcc gggaggcaag gtcgaggcgc tcaactgggt cacgcccgac
     1321 ggcctgtccg tcaagccgct ctacaccgcc gccgacacga acgggctgcc gtacaccgac
     1381 acgctgccgg gcttcgagcc cttcgtccgc ggcccgcagc cgacgatgta cgcgggccgg
     1441 ccgtggacca tccgccagta cgccggcttc tcgaccgccg aggagtcgaa cgcgttctac
     1501 cgccagtccc tcgcgggggg ccagcagggc atcagcgtgg cgttcgacct ggcgacgcac
     1561 cgcggctacg acagcgacca cccgcgcgtc acgggcgacg tcggcaaggc gggcgtggcg
     1621 gtggactcgg tcgaggacat gaagatcctc ttcgacggca tcccgctcga caaggtca
//
LOCUS       DAOSIF010000617         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_62782_length_1674_cov_2.954910, whole genome shotgun sequence.
ACCESSION   DAOSIF010000617 DAOSIF010000000
VERSION     DAOSIF010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62782_length_1674_cov_2.954910"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLT35_14460"
     CDS             <1..888
                     /locus_tag="PLT35_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOG30476.1"
                     /translation="PGSFSEHLLPDHLEHVSLSFLVDTMEKRRGGCGPNIAYTLALLG
                     ERPVLMGTAGRDFADYRQWLDRNGVDTSLVKEIDGKFTASFFCNTDRGGNQIASFYTG
                     AMAHAAELSFRDAGGCDLAIISPNDPAAMTGYARECRALGVPYIYDPSQQVARLSGGD
                     LRDGVAGSRAVICNEYEFQIVREKTGLDEAAMLSAAEAVVVTKGDQGAAIHLRERTIR
                     VDAVPPCEVIDPTGVGDAFRGGMLKGLALGADWATCGRLGSVAAAFALEHLGGSSHAY
                     TWPEFKARYERHFGPLTAG"
     gene            885..1391
                     /locus_tag="PLT35_14465"
     CDS             885..1391
                     /locus_tag="PLT35_14465"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOG30477.1"
                     /translation="MKRLPLLLVWALAVCWAAWLAAVPALAASSPRGAILWAVAATYQ
                     AGGIICHQRDTRSFHVAGVRMPVCSRCAGLYVGAAAGASAVIAWAGTVGGRPRRRVPL
                     RALRAAVAASAAPTLAAWLAEHAAGVWVPDLARALAALPLGAAVAALIALWAGGASFD
                     DAAGSEVD"
     gene            1396..>1674
                     /locus_tag="PLT35_14470"
     CDS             1396..>1674
                     /locus_tag="PLT35_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30478.1"
                     /translation="MAARQAQKKRPAPPPAPVKPRWLVYLLGWLVPGGAHLWLGRPRT
                     GVVFLVLLPFMFALGLAFEGRLFPFDVAEPLTALEARIGELLGAVTELQ"
BASE COUNT          200 a          595 c          616 g          263 t
ORIGIN      
        1 ccgggcagct tctcggagca cctgctgccc gaccacctgg agcatgtgag tctgagcttt
       61 ctcgtggaca cgatggagaa acggcgcggc gggtgcgggc ccaacatcgc gtacaccctg
      121 gcgctgctcg gcgagcgccc ggtgctcatg ggcaccgccg gccgggactt cgccgactac
      181 cgccagtggc tcgacagaaa cggggtggac acctccctgg tgaaggagat cgacgggaag
      241 ttcaccgcgt cgttcttctg caacacggat cgcggcggca accagatcgc ctccttctac
      301 accggcgcga tggcccacgc ggccgagctc tcgtttcgcg acgccggggg ctgcgatctg
      361 gccatcatct cgccgaacga tccggccgcg atgaccggct acgcgcggga gtgccgcgcc
      421 ctcggggttc cgtacatcta cgacccgagc cagcaggtgg cgcgcctgtc ggggggcgac
      481 ctgcgggacg gcgtggccgg ctcgcgcgcc gtgatctgca acgagtacga gttccagatc
      541 gtccgcgaaa agaccgggct cgacgaggcc gccatgctga gcgccgctga ggcggtggtc
      601 gtgaccaagg gcgaccaggg cgccgccatc cacctccggg agcggaccat ccgcgtggac
      661 gcggtgcctc cctgcgaggt tatcgatccg accggcgtcg gcgacgcgtt ccggggcggc
      721 atgctgaagg gactggccct cggggccgac tgggcgacct gcggccgcct cgggagcgtg
      781 gcggcagcct tcgcgctcga gcacctgggc ggttcgagcc atgcctacac atggcccgaa
      841 ttcaaggcgc gctacgaacg gcacttcggg ccgctcacgg ccggatgaag cggctgcctc
      901 tccttcttgt ctgggctctg gcggtctgct gggccgcctg gctcgccgcc gtcccggcgc
      961 tggccgcctc gtcgccgcgg ggcgcgatct tgtgggcggt ggccgcgacg taccaggcgg
     1021 gcggcatcat ctgccaccag cgcgacacgc gctccttcca cgtggccggc gtccggatgc
     1081 cggtgtgttc gagatgcgcc ggcctgtacg tgggggccgc cgctggcgca tcggccgtca
     1141 tcgcgtgggc gggaaccgtc ggcgggcggc ctcggcggcg cgtgcctctg agggcgctcc
     1201 gggccgccgt ggcggcgagc gccgcgccga cgctcgcggc gtggctggcc gagcacgccg
     1261 cgggtgtctg ggtgcccgat ctcgcgcggg cgcttgccgc gcttccgctt ggcgcggccg
     1321 tcgccgcgct gatcgcgctc tgggccggcg gcgcgtcttt cgacgacgcg gcggggagtg
     1381 aggtagactg aggccatggc cgcacgacag gcccagaaga agcgacccgc tccgcctccc
     1441 gcaccggtca agccgcgctg gctcgtctac ctcctcggct ggctggttcc gggcggcgcc
     1501 cacctctggc tcgggcgccc gcggacgggc gtcgtcttcc tggtgcttct gcctttcatg
     1561 ttcgcgctcg ggctggcgtt cgaaggccgg ctgttcccgt tcgacgtcgc cgagccgctg
     1621 accgcgctgg aggcccgcat cggtgagcta ctcggtgccg tcaccgaact gcag
//
LOCUS       DAOSIF010000618         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_63078_length_1669_cov_2.915118, whole genome shotgun sequence.
ACCESSION   DAOSIF010000618 DAOSIF010000000
VERSION     DAOSIF010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63078_length_1669_cov_2.915118"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLT35_14475"
     CDS             <1..838
                     /locus_tag="PLT35_14475"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOG30479.1"
                     /translation="PDTEVSFQIEENIRGTDVFVVQPTCPPVDQHIMELLVMIDAFRR
                     SSAARITAVIPYYGYARQDRKDKPRVPISAKLVANILSASGANRVLTMDLHKAQIQGF
                     FDIPVDHLFAAPVIIDHLARQQFRKLTIVSPDAGGAERARAYAKRLDAELAIIDKRRA
                     PEDGHAEVMNVIGEVRGRTCIIQDDILDTGGTMQNAAQALVANGAERVWACAVHGLFS
                     GPAIQRIEEAPIERFIVTNTIPLSPEKASCRKLVPLSVARLLGQAIRSIHEETSVSSL
                     FV"
     gene            902..1579
                     /locus_tag="PLT35_14480"
     CDS             902..1579
                     /locus_tag="PLT35_14480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOG30480.1"
                     /translation="MEARLDAVKRVTFGKNEARRLRAKGQIPAVLYGGPATGGAPEAT
                     PIAVDPKRLMAILRGESGVNTLIGLTVDGEDVRVLMREYQVDPVTRRLLHADFYRIAM
                     DRMITVTVPVTVTGEARGVKQQGGLLDIVHREVEVQCLPANIPEHIEIDVAELMLGQA
                     IRLRDVATSPTWTPVTDPDVMLVHVIAPRAAAEPEPAEAAAAEAPAAAEPEVIKKGKP
                     EKADEEQ"
BASE COUNT          280 a          551 c          624 g          214 t
ORIGIN      
        1 cccggacacc gaggtctcgt tccagatcga ggagaacatc cgcggcacgg acgtgttcgt
       61 cgtgcaaccg acctgcccgc cggtggacca gcacatcatg gagctgctgg tgatgatcga
      121 cgcgttccgg cgctcgtcgg cggcgcggat cacggcggtc atcccctact acggctacgc
      181 gcggcaggat cgcaaggaca agccccgcgt gccgatctcg gcgaagctcg tggcgaacat
      241 cctcagcgcg tcgggggcga accgcgtgct gaccatggac ctgcacaagg cgcagatcca
      301 gggctttttc gacatcccgg tggaccacct gtttgcggcg ccggtgatca tcgaccacct
      361 ggcgcggcag cagttcagga agctgaccat cgtgtcgccc gacgcgggcg gcgcggagcg
      421 ggcgcgggcg tacgcgaagc ggctggacgc ggagctggcc atcatcgaca agcggcgtgc
      481 gccggaggac gggcacgcgg aggtgatgaa cgtcatcggc gaggtgcggg ggcggacctg
      541 catcatccag gacgacatcc tcgacaccgg gggcacgatg caaaacgcgg cgcaggcgct
      601 cgtcgcaaac ggcgccgagc gcgtctgggc gtgcgcggtc cacggcctgt tctcgggccc
      661 ggcgatccag cgcatcgagg aggcgccgat cgagcggttc atcgtgacca acacgattcc
      721 cctctcgccc gagaaggcct cgtgccggaa gctcgtgccg ctgtcggtgg cccggctgct
      781 cggccaggcg atcaggagca tccacgagga gacctcggtg tcgtcgttgt tcgtgtagac
      841 gaggccctgc ggcggcgggc gggcgttccc cgccgccggc gcaggcagca aggacggaag
      901 catggaagcc aggctcgacg cggtgaagcg ggtaacattc ggcaagaacg aggcgcggcg
      961 gctgcgcgcg aagggccaga tccccgcggt cctctacggc ggccccgcga cggggggcgc
     1021 gccggaggcg acgccgatcg ccgtcgaccc caagcggctg atggccatcc tccgcggcga
     1081 gtcgggcgtc aacacgctca tcggcctgac ggtggacggc gaggacgtca gggtcctgat
     1141 gcgcgagtac caggtcgacc cggtcacgcg ccgcctgctg cacgccgact tctaccgcat
     1201 cgcgatggac aggatgatca ccgtgaccgt gcccgtcacc gtcaccggcg aagccagggg
     1261 cgtgaagcag cagggcggcc tgctggacat cgtgcaccgc gaggtggagg tccagtgcct
     1321 gccggcgaac atcccggagc acatcgagat cgacgtcgcc gaactgatgc tcggccaggc
     1381 catccgcctg cgggacgtgg cgacgagccc gacgtggacg ccggtgacgg accccgacgt
     1441 catgctggtg cacgtgatcg cgccgcgcgc ggcggcggag ccggagccgg ccgaggcggc
     1501 ggcggccgag gcgccggcgg cggccgagcc cgaggtcatc aagaagggca agcctgagaa
     1561 ggctgacgaa gagcagtagt gcggatcgtc gtcgggctcg gcaatccggg gcgccgctac
     1621 cagggaacgc ggcacaacgt cgggttcgcc gtggccgacg aagtggcgg
//
LOCUS       DAOSIF010000619         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_63442_length_1662_cov_3.254512, whole genome shotgun sequence.
ACCESSION   DAOSIF010000619 DAOSIF010000000
VERSION     DAOSIF010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63442_length_1662_cov_3.254512"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLT35_14485"
     CDS             <1..214
                     /locus_tag="PLT35_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30481.1"
                     /translation="TPMYNPVSHLVYVTRGDDVRTTVVNGRILMRDRRMLTLNAPSVL
                     ADARRLAARVREAVEAAKPPQSSTAK"
     gene            complement(193..720)
                     /gene="hpt"
                     /locus_tag="PLT35_14490"
     CDS             complement(193..720)
                     /gene="hpt"
                     /locus_tag="PLT35_14490"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOG30482.1"
                     /translation="MAYEAPKNILIPAEDIQRRVRELAFEIGRDYADDAQLHFVSVLK
                     GAFVFLGDLVRSMDRGVSLDFMAVSSYGKSVNSSGEVRLLKDLDSGLEGRNVIIVEDI
                     VDTGLTLAYLQEILRARGPKSLRTACLLSKPSRRKVDVKVDYIGFTIEDRFVVGYGLD
                     YAEQYRHLPYLAVLD"
     gene            complement(802..875)
                     /locus_tag="PLT35_14495"
     tRNA            complement(802..875)
                     /locus_tag="PLT35_14495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(841..843),aa:Gly,seq:ccc)
     gene            complement(944..>1662)
                     /locus_tag="PLT35_14500"
     CDS             complement(944..>1662)
                     /locus_tag="PLT35_14500"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HOG30483.1"
                     /translation="IEENRRDLVERVAAERADLGIAWDGDADRCFFVDRDGEFVPGDF
                     VTALLAEAFLRKEAGGTIVYDLRASYAVRDTVARFGGRALMNRVGHAFFKKRMREENA
                     VFGGEVTGHYYFRDNFYADNGFIPAMLILELMSVKGQSMHQLLEPLRAKYFISGEINT
                     KVKSMELVEEKLEGLAAMGAAIPGAQVYRLDGVSVEAPGWHFNVRPSNTEPLLRLNLE
                     ADTPELMAQKRDEVLGFIRS"
BASE COUNT          275 a          565 c          550 g          272 t
ORIGIN      
        1 gacgccgatg tacaacccgg tgtcccacct cgtctacgtc acaaggggcg atgacgtccg
       61 cacgaccgtc gtgaacggcc ggatcctgat gcgcgatcgg cggatgctga ccctgaacgc
      121 gccctcggtc ctcgccgatg cccggcgcct ggccgcgagg gtccgcgagg ccgtcgaggc
      181 cgcgaagccg cctcagtcga gcacggccaa gtagggcagg tggcggtact gctcggcgta
      241 gtcgagaccg tagccgacga cgaagcggtc ctcgatcgtg aagccgatgt agtcgacctt
      301 gacgtccacc ttccgcctcg agggcttgct gagcaggcag gcggttctca gcgacttcgg
      361 gccccgggcg cgcaggatct cctgcaggta ggccagggtc agcccggtgt ccacgatgtc
      421 ctcgacgatg atcacgttcc ggccctcgag gcccgagtcg aggtctttca ggaggcgcac
      481 ctctccggac gaattgacgc tcttcccgta gctcgacacc gccatgaagt cgaggctcac
      541 gccgcggtcc atggatcgga cgagatcgcc caggaagacg aaggctccct tcagcacgct
      601 cacgaagtgc agctgcgcat catcggcgta gtcgcgtccg atttcgaagg ccagttcccg
      661 gacgcgccgc tgaatgtcct cggctggaat caggatgttc ttgggtgcct cgtacgccat
      721 cgggtgacag cctagcacag gccggggcgc cgggaaccga aacacggcct cgccgggcgg
      781 ctgacgggct ctgcaggggg atggagcggg ctacgggaat cgaacccgtc tagctggctt
      841 gggaagccag agcattacca ctatgctaag cccgcccgcc ggacgcgcga gttcccattc
      901 tagcagactc cggccgccgc gactcgcccc tggcgcctgg cgcctagctc ctgatgaacc
      961 ccaacacctc gtcccgcttc tgggccatca actcgggagt gtccgcctcg aggttcaggc
     1021 gcagcagggg ctcggtgttc gacgggcgca cgttgaagtg ccagcccggc gcctcgaccg
     1081 acacgccgtc caggcggtag acctgcgcgc cgggaatggc ggctcccatg gcggcgaggc
     1141 cctcgagctt ctcctcgacg agctccatcg acttcacctt ggtgttgatc tcgcccgaga
     1201 tgaagtactt cgcccgcagg ggctccagga gctggtgcat ggactggccc ttcacggaca
     1261 tcagttcgag aatgagcatc gcgggaatga agccgttgtc ggcgtagaag ttgtcgcgga
     1321 agtagtagtg gcccgtgacc tcgccgccga acacggcgtt ctcctcgcgc atgcgcttct
     1381 tgaagaacgc gtggcccacg cggttcatca gcgcccggcc gccgaagcgc gccacggtgt
     1441 cccgcacggc gtagctcgcc cgcaggtcgt agacgatcgt cccgccggcc tctttgcgga
     1501 ggaaggcttc cgccagcagg gccgtgacga agtcgcccgg cacgaactcg ccatcgcggt
     1561 ccacgaagaa gcagcggtcg gcgtcgccgt cccaggcgat gccgagatcc gcgcgctcgg
     1621 cggcgacgcg ctcgacgagg tcccggcggt tctcctcgat ga
//
LOCUS       DAOSIF010000620         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_63535_length_1660_cov_3.737072, whole genome shotgun sequence.
ACCESSION   DAOSIF010000620 DAOSIF010000000
VERSION     DAOSIF010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63535_length_1660_cov_3.737072"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLT35_14505"
     CDS             complement(<1..443)
                     /locus_tag="PLT35_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30484.1"
                     /translation="MTIDRAARLAVYTDIVQRLSGALRAASLYSIAHPTVAEHVRGLL
                     EALERIHRAEATVLIGFIGGEVIADDTPLLAVTAYRTELVRYMQALGINRVLVERGVT
                     LDELTAFVQAVSHPSPAALRRLEEGGQEAEDTDFLRLDHLRAGRIP"
     gene            complement(440..>1660)
                     /locus_tag="PLT35_14510"
     CDS             complement(440..>1660)
                     /locus_tag="PLT35_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30485.1"
                     /translation="VPDAQRRRLIVSEAQDVALGAEALEQWAALERNLDKQSDARFVP
                     ETYVEEIHAVQDRAGRRVRRDGIPPARLAAWLRSIGEDAVRDADLRMLADLAQVESDP
                     IRARRVLELLQAHVLEAADLGDWDGAARTAEVVRGLASTAIDETRRRGALEVLERLSQ
                     SAAVAKAYDSLADADEQASARLVRLLSAAGPAMVPVLVPRWAAESRKAVRSRFEQAVV
                     GCGKPGRDALRRLLATEGADAAIRIAAVRLLAMTPGDEHLPLLESALSNRHQGMRDVA
                     FDVLAHAPLERARDILARGIARADADVQAALLERLRGLGPDLAVPVFRRLFGCVDPRT
                     ADLRPLAAMIASLGRVGGGSARPLLADVARRVSWRTPLRAWRVRSAVASALRETESRA
                     APVGRPTAAGGGSA"
BASE COUNT          238 a          619 c          594 g          209 t
ORIGIN      
        1 gggatgcggc ccgcccgcag gtgatcgagg cgcaggaaat cagtgtcttc cgcctcctgg
       61 cccccttcct ccagcctgcg cagcgccgcg ggagacggat gcgagacggc ctggacgaag
      121 gcggtgagtt cgtcgagcgt cacgccgcgc tcgacgagaa cccggttgat gccgagcgcc
      181 tgcatgtagc ggaccagttc ggtgcggtac gccgtgacgg cgagcagcgg cgtgtcgtcg
      241 gcaatcacct cgccgccgat gaagccgatg agcaccgtag cctcggctcg gtggatgcgc
      301 tccagggctt ccaggagccc ccggacgtgc tcggccacgg tgggatgggc gatcgaatag
      361 aggctcgcgg cccggagcgc gccgctcagc cgttggacga tgtccgtgta cacggcgagc
      421 cgcgccgcgc gatcgatggt catgcggaac ccccgccagc cgcggtcggg cgcccgacag
      481 gcgccgcgcg cgattccgtt tcgcgaagcg ccgaggcgac cgccgacctg acccgccatg
      541 cccgcaacgg cgtccgccac gacacccggc gggcgacatc cgcgaggagc ggccgggcgc
      601 tcccgccgcc cacgcggccg agcgacgcga tcatcgcggc gagcggccga aggtcggccg
      661 tccgcggatc gacacagccg aacaggcggc ggaacacggg cacggccagg tcgggcccga
      721 gccctctcag gcgctcgagc agcgccgcct gcacgtccgc gtccgccctg gcgatgccgc
      781 gggccaggat gtccctggcc cgctcgagcg gagcgtgcgc gagcacgtcg aacgccacgt
      841 cacgcatgcc ctggtgccgg ttcgacagcg cggactccag gagcggcagg tgctcgtcgc
      901 cgggcgtcat ggcgagcagc ctgacggcgg caatgcggat ggcggcgtcc gcgccctcgg
      961 ttgccagaag gcggcggagg gcatcgcgcc ccggcttccc gcagccaacg accgcctgct
     1021 cgaagcgcga gcgaaccgcc ttccggctct ccgcggccca ccgcggcacg agtacgggca
     1081 ccatcgccgg ccccgctgcc gacaacaggc ggaccagccg ggcagacgcc tgctcatcgg
     1141 catcggccag gctgtcgtag gccttggcga ctgctgcgga ctgggacagc cgctcgagta
     1201 cttccagagc tcccctcctg cgggtctcgt cgatcgcggt cgaggccagg ccccggacca
     1261 cttcggcggt ccgggcagcg ccgtcccagt cgccgagatc cgccgcctcg agcacgtgag
     1321 cctggagcag ctccaggacc cgccgggccc ggatcggatc agactcgact tgcgccagat
     1381 ccgcgagcat gcgcaagtcc gcgtcccgga ccgcgtcctc tccgatcgag cgaagccatg
     1441 cggccaggcg cgctggcggg atgccgtcac gccggacacg gcggcccgcc cggtcctgga
     1501 ccgcgtggat ctcctcgacg taggtttcgg gcacgaaccg cgcgtccgac tgcttgtcga
     1561 gattgcgctc gagcgctgcc cactgctcca gggcctcggc gccgagcgcg acatcctggg
     1621 cctcgctgac gatcaggcgc cggcgttggg cgtcgggcac
//
LOCUS       DAOSIF010000621         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_63637_length_1658_cov_3.160324, whole genome shotgun sequence.
ACCESSION   DAOSIF010000621 DAOSIF010000000
VERSION     DAOSIF010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63637_length_1658_cov_3.160324"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1544)
                     /gene="glmM"
                     /locus_tag="PLT35_14515"
     CDS             complement(201..1544)
                     /gene="glmM"
                     /locus_tag="PLT35_14515"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG30486.1"
                     /translation="MNTRLFGTDGVRGRAGAFPLDARTVRRLGAALVRALPDRGGGPA
                     LLIGRDTRESGARIERDLAFGASYAGATVVSAGVMPTPAVAHLTRSEGYAAGLVVSAS
                     HNPYYDNGIKVFLGGGEKVTEALEARIEAIVADASWDAPGGDAPAVPLVDEAGAYLTH
                     AKQALGGQVPAHGRRIAVDCANGATHEVAPALLHGLGFEVTLAGCSPDGRNINLDCGS
                     THPDALARLVVEEGCDLGVAFDGDGDRAIFVDHAGRIVDGDAVMFLCAKQMLATGRLR
                     GPAVVATVMSNIGLELALGDLGIGLVRCPVGDRHVMEELLRAGLSLGGEQSGHVVFPD
                     LLFTGDGLITALSVLRVMAETGRTLAELAGELTRFPQVLLNVRVREQPDWSAIPAVAA
                     AVADVERRLGARGRLLVRYSGTEPLLRIMIEGERQDDIGAWAERIAAAARDAIGA"
BASE COUNT          204 a          661 c          587 g          206 t
ORIGIN      
        1 tgccccagtc gagctggtgg gcgcccttga gcgggaagcc ttcgtgggcc gccggaacgt
       61 ccggatggaa ctgcttggtc tcgctcgccc cgtcgatgtc agccatggcc attctccgtc
      121 ttgaccgcgc gccgcggccg ggcccgccgc ccgtccgcca ctcgcgctgc tcccgcggcc
      181 gcaccggcgc ggccgcggcc tcacgccccg atggcgtccc gagccgccgc ggcgatccgc
      241 tcggcccacg ccccgatgtc gtcctggcgc tcgccctcga tcatgatgcg caggagcggc
      301 tcggtccccg agtagcggac cagcaggcgg ccccgcgcgc ccagccgccg ctcgacgtcg
      361 gcgacggccg ccgcgacggc cgggatcgcc gaccagtccg gctgctcgcg cacgcgcacg
      421 ttcaggagca cctgcgggaa ccgcgtcagc tcgccggcca gctcggccag ggtccggccg
      481 gtctcggcca tcacgcgcag gacgctcagc gcggtgatca ggccgtcgcc ggtgaagagc
      541 aggtccggga agaccacgtg ccccgactgc tcgccgccga gcgacagccc ggcgcgcagg
      601 agctcctcca tcacgtgcct gtcgcctacc gggcagcgca cgagcccgat gccgagatcg
      661 ccgagcgcca gctccaggcc gatgttgctc atcaccgtgg ccacgaccgc cgggccgcgg
      721 agccgcccgg tcgcgagcat ctgcttcgcg cacaggaaca tcaccgcgtc gccgtcgacg
      781 atgcggccgg cgtgatccac gaagatcgcg cggtcgccgt cgccgtcgaa cgccacgccg
      841 agatcgcagc cctcctcgac gacgagccgc gccagggcgt ccgggtgcgt ggacccgcag
      901 tcgaggttga tgttgcggcc gtcggggctg cagccggcca gcgtgacctc gaacccgagg
      961 ccgtgcagga gcgccggcgc cacctcgtgc gtcgccccgt tggcgcagtc cacggcaatg
     1021 cgcctgccgt gcgccggcac ctgtccgccg agcgcctgct tcgcgtgcgt gaggtaggcg
     1081 cctgcctcgt cgacgagcgg caccgctggc gcgtcgccgc cgggcgcgtc ccacgaggcg
     1141 tcggccacga tcgcctcgat ccgggcctcg agcgcctcgg tgaccttctc gccgccgccc
     1201 aggaagacct tgatgccgtt gtcgtagtag ggattgtgcg acgccgagac gaccaggccg
     1261 gcggcgtacc cttcgctgcg cgtcaggtgg gcgacggctg gcgtcggcat gacgcccgcg
     1321 ctgacgaccg tggcccccgc ataggatgcg ccgaacgcca ggtcgcgctc gatgcgggcg
     1381 cccgactccc gcgtatcgcg cccgatgagc agcgcggggc cgccgcctcg gtccggcagc
     1441 gccctcacga gcgccgcgcc cagccgcctg acggtgcgcg cgtcgagggg aaacgccccg
     1501 gcccggccgc ggacgccgtc cgtgccgaac agccgcgtgt tcatcggatg gccacccgga
     1561 cctcgtgcgg cgcgacggcc accacctcga tccccggcgg cgcgctgacg cggaccgcga
     1621 cggtgtggcg ccccgccgca aggcccgcca ggctggcg
//
LOCUS       DAOSIF010000622         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_63733_length_1656_cov_3.811993, whole genome shotgun sequence.
ACCESSION   DAOSIF010000622 DAOSIF010000000
VERSION     DAOSIF010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63733_length_1656_cov_3.811993"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1656)
                     /locus_tag="PLT35_14520"
     CDS             complement(<1..>1656)
                     /locus_tag="PLT35_14520"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG30487.1"
                     /translation="LKEVADADGLVVLFIDELHTVVGAGAAEGAIDASNMLKPMLARG
                     ELHTIGATTLDEYRKHIEKDAALERRFQPVLVDQPSVEDTISILRGLRERYEIHHGVR
                     LKDTALVAAAVLSQRYITDRFLPDKAIDLVDEAAARLRMEIDSMPAELDEVERRVMQR
                     EIEAQALRKEPDPASAERLERLERELADLKEAGHRLRAHWQQEKDAIQDARARKQELE
                     QLRHEIDRAQRAGDFATASELQYGRVPDTERRIREAEERLADLQRDTKMLKEEVDEED
                     IAEVVSRWTGVPVSRLMEGEIQKLVRMEERLHQRVVGQDDAIGSVANAIRRARAGLQD
                     PHRPLGSFLFLGPTGVGKTELARALAEFLFDDEQAMIRIDMSEYQEKHTVSRMVGAPP
                     GYIGHDEAGQLTEAVRRRPYAVVLFDEVEKAHADVLNVLLQLLDDGRLTDSKGRTVDF
                     KNAVVIMTSNLGSQYLAERAGQAADAVDDGTRRRVWDALRAHFKPEFLNRIDEIIFFH
                     SLDGALAQARHPANAAGSAGPADPARRVRRRRHHPRRGGGRRPG"
BASE COUNT          215 a          600 c          552 g          289 t
ORIGIN      
        1 ccaggtcgtc gcccgccgcc tcgacgcgga tggtgtcgcc gtcggcgaac tcgccttgca
       61 ggatccgcag ggccagcgga tccagcagca ttcgctggat ggcgcgcttg agcgagcgcg
      121 ccatccagcg aatggaagaa gatgatctcg tcgatccggt tgaggaactc cggcttgaag
      181 tgcgcgcgca gcgcgtccca gacacgccgc cgcgtcccgt cgtccaccgc gtcggctgcc
      241 tgcccggcgc gctcggccag gtactggctg cccaggttgg acgtcatgat cacgacggcg
      301 ttcttgaagt ccaccgtgcg tcccttcgag tcggtgagcc ggccgtcgtc caggagctgg
      361 agcagaacgt tcagcacgtc ggcgtgcgcc ttctcgacct cgtcgaagag cacgacggcg
      421 tagggccggc ggcggacggc ctccgtgagc tggcccgcct cgtcatggcc gatgtagccc
      481 ggaggcgctc cgaccatccg cgagacagtg tgcttctcct ggtactcgga catgtcgatc
      541 cggatcatgg cctgctcgtc gtcgaacagg aactccgcca gcgcgcgcgc cagctccgtc
      601 ttgccgacgc ccgtcgggcc gaggaacaga aagctgccaa gcgggcggtg cgggtcctgc
      661 aacccggctc gcgcgcgccg gatcgcgttg gccaccgatc cgatggcgtc gtcctggccc
      721 accacccgct ggtgcaggcg ctcctccatc cgcacgagct tctggatttc accctccatg
      781 agacggctga ccggcacgcc cgtccagcgg ctgacgactt cggcgatgtc ctcttcgtcc
      841 acctcctcct tcagcatctt cgtgtcgcgc tggagatcgg ccagtcgctc ctcggcctcg
      901 cggatgcgcc gctccgtgtc cggcacgcgg ccgtactgca gctccgacgc cgtggcgaag
      961 tccccggcgc gctgcgcgcg gtcgatctcg tgccgcagct gctcgagttc ctgcttgcgg
     1021 gcgcgcgcgt cctggatggc gtccttctcc tgctgccagt gggcgcggag ccgatgtccc
     1081 gcctccttga gatccgccag ctcgcgctcg aggcgctcca gacgctcggc cgaggcggga
     1141 tcgggctcct tgcggagcgc ctgcgcctcg atctcgcgct gcatcacccg gcgttcgacc
     1201 tcgtccagct ccgctggcat cgagtcgatc tccatgcgga gccgggccgc cgcttcgtcg
     1261 acgagatcga tggccttgtc gggcaggaag cggtccgtga tgtaacgctg cgacagcacc
     1321 gcggccgcca ccagggccgt gtccttcagc cggacgccgt ggtggatctc gtaccgctcg
     1381 cgcaggccgc gcaggatgct gatggtgtcc tcgacgctcg gctggtccac cagcaccggc
     1441 tggaagcgcc gctcaagtgc cgcgtccttc tcgatgtgct tgcggtactc gtcgagggtc
     1501 gtcgcgccga tggtgtgcag ctcgccccgc gcgagcatcg gcttgagcat gttggacgcg
     1561 tcgatggcgc cctcggcggc gccggcgccg accaccgtgt gcagctcgtc gatgaagagc
     1621 accaccaggc cgtcggcgtc ggcgacctcc ttcagc
//
LOCUS       DAOSIF010000623         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_64010_length_1651_cov_2.867794, whole genome shotgun sequence.
ACCESSION   DAOSIF010000623 DAOSIF010000000
VERSION     DAOSIF010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64010_length_1651_cov_2.867794"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PLT35_14525"
     CDS             <1..1584
                     /locus_tag="PLT35_14525"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOG30488.1"
                     /translation="RHGTVERLNAAWYRTFTAWDQVEPPRFGTILTYADFMDWRVFIG
                     DKIAADLRSRADAVRAVDASHPVTSHAPNPSPVFRTLADSMDASDDYLMKSSVDYFGT
                     SFYPKLTSPDRDFPLERRALVFDMARAVTGGRGFYIGELQAGYGVHGIVTGSPITPRD
                     LEIYAWSAVARGAKSISFYAYYPMSTGYEAGGYGLVNLDGSLTERSRRAGAAARAIAA
                     NADLILAASPVQPRVAVVFNPLVPLLGGEQAYGDRRSMHRAVAGYHRMFFERNIPVDF
                     PSARELSPAGLASYALVIVPCPILMTAEMAGALEAYARNGGRLFIEARPGWQDERGRA
                     APVLPAFGWDRMLGVRESEVLPVKQVQVRWGSRAFPGTAVAERFAVQDASASAVAAFD
                     DGAPAAFERAVGKGRAIVLGTFAGESNALDPVAMNPLGDILAEWAGLERPALEASSFV
                     EIRRMAAPDGELVFLFNHGPQAARVALELPLARAAALVREITAGEPAPGRLAGPSDAL
                     RLKGEIPAQSARVYRVDYR"
BASE COUNT          229 a          590 c          604 g          228 t
ORIGIN      
        1 aggcacggca ccgtcgagcg gctgaacgcg gcctggtacc ggaccttcac cgcctgggac
       61 caggtggagc cgccgcgctt cgggacgatc ctgacgtacg cggacttcat ggactggcgc
      121 gtgttcatcg gcgacaagat cgccgccgat ctgcggtcgc gggccgacgc cgtgcgggcc
      181 gtcgacgcgt cgcaccccgt gacgagccac gctcccaacc cctcgcccgt gttccggacg
      241 ctcgccgaca gcatggatgc ctccgacgac tacctgatga agtcgtcggt cgactacttc
      301 gggacgagct tctacccgaa gctcacgtcg ccggaccggg acttcccgct ggagcggcgg
      361 gcgctggtgt tcgacatggc gcgggccgtc accggcggcc gcggcttcta catcggcgag
      421 ctgcaggccg gctacggcgt ccacggcatc gtcaccggca gccccatcac cccgagagac
      481 ctggagatct acgcgtggag tgccgtcgcg cgcggggcga agtcgatcag cttctacgcg
      541 tactatccga tgagcacggg atacgaagcg ggcggctacg gcctggtcaa cctggacgga
      601 tcgctgaccg agcgcagccg ccgggcaggc gcggcggcgc gcgcgatcgc ggcgaacgcc
      661 gatttgattc tggcggccag tcccgtgcag ccgcgcgtcg ccgtcgtctt caacccgctc
      721 gtgccgctcc tcggcggcga acaggcgtac ggcgaccggc gctcgatgca ccgcgccgtg
      781 gccggctacc accgcatgtt cttcgagcgg aacatcccgg tcgacttccc cagcgcgcgc
      841 gagctgagcc cggccgggct ggcgagctac gccctcgtga tcgtgccctg cccgatcctc
      901 atgaccgcgg agatggccgg cgcgctggaa gcgtacgccc ggaacggcgg gcgcctcttc
      961 atcgaggcga ggcccggctg gcaggacgag cgcgggcgcg ccgcacccgt gctgcccgcg
     1021 ttcggctggg accggatgct cggcgtgcgc gagtcggaag tgctgcccgt gaagcaggtg
     1081 caggtccgat ggggatcgcg cgccttcccc ggcacggccg tcgccgagcg gttcgccgtg
     1141 caggacgcgt cggcatcggc cgtggcggcg ttcgacgacg gtgcgccggc ggccttcgaa
     1201 cgcgccgtcg ggaagggccg cgcgatcgtc ctcgggacct ttgccggcga gagcaacgcc
     1261 ctcgacccgg tggcgatgaa ccccctcggc gacatcctgg ctgagtgggc gggcctcgag
     1321 cggcccgcgc tcgaggcgtc gagcttcgtc gagatccgcc gcatggcggc gcccgacggc
     1381 gaactggtgt tcctgttcaa ccatgggccg caggcggccc gcgtggcgct cgaactgccg
     1441 ctggcccgcg ccgcggcgct ggtccgcgag atcacagccg gcgaaccggc gccaggccgg
     1501 ctcgcaggcc cgtccgacgc actcaggctc aaaggagaga tccccgcgca gagcgcgagg
     1561 gtttacaggg tggactaccg gtaggtgcgt acgcacgccg acacgcccgc cggaggcggg
     1621 cgcgtcgacg ggcaggatcg cgggcggggt g
//
LOCUS       DAOSIF010000624         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_64751_length_1637_cov_2.717446, whole genome shotgun sequence.
ACCESSION   DAOSIF010000624 DAOSIF010000000
VERSION     DAOSIF010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64751_length_1637_cov_2.717446"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="hydA"
                     /locus_tag="PLT35_14530"
     CDS             <1..1248
                     /gene="hydA"
                     /locus_tag="PLT35_14530"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOG30489.1"
                     /translation="PGGIDAHTHLDMPAGAIRSSDDFETGTMAAAYGGTTTIIDFATP
                     ERGQELGRALDAWMGLAGGRALIDYAFHMVVPEWRDGTAAEMDRLVQRGVTSFKLFMA
                     YPGRLMLDDGAIFRALRCTRGNGGLVALHAENGAVIDVLVQEALALGRTAPKFHARTR
                     PAGAEGEAIRRAIALAEMAGAPVYIVHVSSAEGVAAIARARERGVPVHAETCPQYLCL
                     SDAEYDRPGFDAAKFVISPPLRPPEDQDALWEGLAEGVLDVVATDHCPFGMDDPPHKR
                     LGLSDFSKIPNGAPGIETRLLLLWDQGVRTGRISPSRFVEVTAAAPAKIFGLWPRKGA
                     IAAGSDADLVLWDPSARTTISASTHHMRVDYSLYEGRTVTGAPVTVVSRGEVIIDRGA
                     VTGRKGRGVYLSRTPRARPAGGP"
     gene            1245..>1637
                     /locus_tag="PLT35_14535"
     CDS             1245..>1637
                     /locus_tag="PLT35_14535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG30490.1"
                     /translation="MSGPPDTLGHDKAPQRIAAMFDAIAPRYDLLNHVLSAGLDLRWR
                     AKAVRSLGLTGGEKIVDLCTGTGDLALALARARPRAGRVVGVDFSGGMLRVGRAKACA
                     RGLADRIRLVRGDVTRLPLADGSADAATV"
BASE COUNT          216 a          588 c          611 g          222 t
ORIGIN      
        1 cccggcggca tcgacgccca cacgcatctc gacatgccgg cgggcgccat ccggtccagc
       61 gatgacttcg agacgggcac gatggccgcc gcctacggcg gcaccacgac catcatcgac
      121 ttcgccacgc cggagcgggg gcaggagttg ggccgggcgc tcgacgcgtg gatgggcctc
      181 gccggcgggc gcgcgctcat cgactacgcc tttcacatgg tggtgccgga atggcgcgac
      241 ggcaccgcgg ccgagatgga ccgcctcgtg cagcgcggcg tcacctcgtt caagctgttc
      301 atggcctacc ccggacgcct gatgctcgac gatggcgcca tcttccgcgc gctgcggtgc
      361 acgcgcggga acggcggctt ggtggcgctg cacgccgaga acggggcggt catcgacgtg
      421 ctcgtccagg aggccctggc gctcggccga accgccccga agttccacgc gcgcacgcgc
      481 ccggccggcg ccgaaggaga agccatccgg cgggccatcg cgctggcgga gatggccgga
      541 gcgcccgtgt acatcgtgca cgtgtcgtcg gcggagggcg tggcggccat cgcccgcgcg
      601 cgggagcgag gcgtgccggt tcatgcggag acctgcccgc agtacctctg cctctcggac
      661 gccgagtacg accggccggg gttcgatgcc gcgaagttcg tcatctcgcc gccgctccgc
      721 ccgcctgaag accaggacgc gctctgggag ggcctcgccg agggcgtcct cgacgtcgtg
      781 gcgaccgacc actgtccctt cggcatggac gacccgccgc acaagcggct cggactgagc
      841 gacttctcga agatcccgaa cggcgcgccg ggcatcgaga cgcgcctgct gctgctgtgg
      901 gatcagggcg tccggaccgg gcggatcagc ccgagccggt tcgtcgaggt gacggcggcc
      961 gccccggcga agatcttcgg tctctggccg cgaaaaggcg ccatcgccgc gggttcggat
     1021 gcggacctcg tgctgtggga tccgtcggcg cgcacgacga tctcggcctc cacccaccac
     1081 atgcgcgtgg actactctct ctacgagggc cgcaccgtca cgggcgcgcc cgtcaccgtc
     1141 gtgtcgcgcg gcgaggtgat catcgaccgc ggcgcggtca cgggccggaa gggccgcggc
     1201 gtgtacctgt cgcgcacgcc tcgcgcacgg ccggcgggcg gaccgtgagc gggcccccgg
     1261 acacgctcgg gcacgacaag gcgccgcagc ggatcgcggc gatgttcgac gcgatcgcgc
     1321 cgcgctacga cctgctcaat cacgtcctca gcgccggctt ggacctgcgc tggcgggcga
     1381 aggccgttcg gtcgctgggg ctgaccggcg gcgagaagat cgtcgatctt tgcaccggaa
     1441 ccggggacct cgccctcgcg ctggcgcgcg cccggccgcg ggccggccgg gtggtcggcg
     1501 tcgacttctc aggcggaatg ctccgggtgg gccgggccaa ggcgtgcgca cgcggcctgg
     1561 cggatcggat ccggctggtg cgcggcgacg tcacgcggct gccgctggcg gacggatccg
     1621 ccgatgcggc cacggtg
//
LOCUS       DAOSIF010000625         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_64790_length_1636_cov_3.765971, whole genome shotgun sequence.
ACCESSION   DAOSIF010000625 DAOSIF010000000
VERSION     DAOSIF010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64790_length_1636_cov_3.765971"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLT35_14540"
     CDS             <1..595
                     /locus_tag="PLT35_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30491.1"
                     /translation="ALLLICSAAVRAQPPVARPQPPPAAPAAKAPAPAPPEAGGAAPP
                     RATVPAARPQDVAAELEPPSDAAAGQDVAPPPESLNAPVYPSAAYLGSYDAGRGQRFF
                     LYGTTQAFEDIVLYYRGVLKGRGDRVFDEPATHMFDVGKYREADVAFPPSVTIKNYAF
                     GGSPGLPNPVPGAQPPYFPTVIQIVTPPPAAAGRRVP"
     gene            complement(622..>1636)
                     /locus_tag="PLT35_14545"
     CDS             complement(622..>1636)
                     /locus_tag="PLT35_14545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOG30492.1"
                     /translation="ALYSDRSQIALRMLTAGEAPAGLDLWRARLASAIALRRALDIDA
                     TACRLVHGEGDLLPSCIVDQYGDYLVVQALSQGADRMLPALVGLLEEQLHPAGILARH
                     DVKARELEGLDSAVQALAGSVPPLVTVREGRIEHEVDLWHGQKTGLFLDQRENRAAAR
                     AYARGRLLDCFSYHGAFALHLADRCDRVVALDVSEDAVARIAINAARNGIATIEARAA
                     NAFDALRDMERARERFDTIVLDPPAFAKNKAAVERARAGYKEINLRAMKLLAPGGTLV
                     TCSCSYHVDEAAFGQIVYEASVDARARMAVMEKRMQARDHPVVLGVPETYYLKCFIMR
                     KLA"
BASE COUNT          214 a          593 c          596 g          233 t
ORIGIN      
        1 cgcgctgctt ctcatctgct cggccgccgt acgcgcgcag ccgccggtcg cccgtccgca
       61 gccgccgccc gccgctccgg ctgcgaaggc gccggctccc gcgccgccgg aggccggcgg
      121 ggccgcgccc ccgcgcgcga cggttcccgc ggcgcgcccg caggacgtgg ctgcggagct
      181 ggagccgccg tccgatgcgg cagccgggca ggacgtggcg ccgccgcccg agagcctgaa
      241 cgcgcccgtc tatccgtcgg ccgcgtacct gggttcctac gacgccggcc ggggccagcg
      301 tttctttctg tacggcacga cccaggcgtt cgaggacatc gtgctgtact atcggggcgt
      361 gctgaagggc aggggcgatc gcgtgttcga cgagcccgcc acgcatatgt tcgacgtcgg
      421 gaagtaccgc gaggccgacg tggcgtttcc gccgagcgtg acgatcaaga actatgcgtt
      481 cggcggatcg cccggcctgc ccaatcccgt tcctggcgcg cagccgccct acttcccgac
      541 ggtgatccag atcgtcacgc cgccgccggc ggctgccggc cgccgggtcc cgtagggggg
      601 cctgtcgccc cgggccgggg gtcaggccag ctttctcatg atgaagcact tgaggtagta
      661 ggtttccggc acgcccagaa ccacggggtg atcgcgggcc tgcatccgct tctccatgac
      721 ggccatgcgc gcgcgggcgt ccacggaagc ctcatagacg atctggccga aggcggcctc
      781 gtccacgtgg taggagcagc tgcacgtcac cagggtcccg cccggcgcga gcagcttcat
      841 ggcgcgcagg ttgatctcct tgtagccggc gcgggcgcgc tccacggccg ccttgttctt
      901 ggcgaaggcc ggcgggtcca gcacgatcgt gtcgaaccgc tcccgcgccc gctccatgtc
      961 gcgcagcgcg tcgaaggcgt tggccgcgcg cgcctcgatc gtggcgatgc cgttgcgcgc
     1021 ggcgttgatc gcgatgcggg cgacagcgtc ctcggagacg tcgagggcga cgacgcggtc
     1081 gcagcggtcg gccagatgca acgcgaaggc gccgtggtag ctgaagcagt cgagcagccg
     1141 gccgcgggcg tatgcgcggg cggcggcgcg gttctcgcgc tggtcgagga acaggccggt
     1201 cttctggccg tgccacaggt ccacctcgtg ctcgatacgg ccttcgcgca ccgtgacaag
     1261 cgggggcacg ctcccggcga gcgcctgcac ggccgagtcc aggccctcca gctcgcgggc
     1321 cttcacgtcg tggcgcgcga ggatgcccgc cgggtgcagc tgctcctcca gcaggccgac
     1381 cagcgcgggc agcatccgat ccgcgccctg cgacagcgcc tgcacgacca ggtagtcgcc
     1441 gtactgatcc acgatgcacg acggcagcag gtcgccttcc ccatggacga ggcgacaggc
     1501 cgtcgcgtcg atgtcgagcg cccgcctgag cgcgatggcg gacgccaggc gagcccgcca
     1561 cagatcgagg ccggccgggg cctcgcccgc ggtgagcatc cgcagcgcga tctgggagcg
     1621 gtcgctgtag agggcg
//
LOCUS       DAOSIF010000626         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_64804_length_1636_cov_2.955724, whole genome shotgun sequence.
ACCESSION   DAOSIF010000626 DAOSIF010000000
VERSION     DAOSIF010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64804_length_1636_cov_2.955724"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLT35_14550"
     CDS             <1..368
                     /locus_tag="PLT35_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30493.1"
                     /translation="ELRFAHFQAREHLGPRRPFPATSLLEGGQRLASALFGMPFGTLE
                     PGSAADLIVADYVSPTPVTAGNLAAHLVAGLQPAMFRRVMAAGRWVCLDGVPANVNVE
                     AVYERASRLAASLWRRMRG"
     gene            361..>1636
                     /locus_tag="PLT35_14555"
     CDS             361..>1636
                     /locus_tag="PLT35_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30494.1"
                     /translation="MAEFVPVPIGRLLRRAHREYSRQGAVFGLPEQKFYRPPALRDAD
                     TAPGPSTLDLSVRVSGQRAGNPMGLAAGPHTQLAQNIALGWLAGARVFELKTVQVNDR
                     ITVPRPCIDAATIGYNTEWSQELRLDESLREYVKASMLIEALEAMGILGRGIGLEGRE
                     SPGGPPPRATPVDGGVVFDASVGYDLEGIRSPAMASWLESIRSAGAIVDDLRREIAAA
                     FPGLRDFPFKTRIAAGVTVSTFHHTPPGEIERIGEFLIGDLGFHTMIKVSPPMLGRER
                     LEAILHGALGYEEVAVDPSVYERCLSFEEAAGIMRRLQPLAARRGVTAGVKCGNTLEV
                     VGAATSLTARVQYLSGQPLHALHAALAAEWREAFGPDLPLAFAGGVDAGNAADCVAAG
                     LAPVTVCTDLLRAGGYGRLPRYLVRLDTRMRAV"
BASE COUNT          215 a          591 c          596 g          234 t
ORIGIN      
        1 cggagctccg gttcgcgcac ttccaggcgc gcgaacacct gggtccccgc cgcccgtttc
       61 cggccacgtc cctgctcgag ggaggccaac gcctggcctc ggcgctcttc ggcatgccct
      121 tcggcacgct ggagccgggc agcgccgcgg acctgatcgt cgccgactac gtgtctccga
      181 cacccgtgac cgccggcaac ctggccgccc acctcgtagc cgggctccag cccgcgatgt
      241 tccgccgggt gatggccgcg ggccgctggg tctgcctcga cggcgtgccc gcgaatgtca
      301 acgtcgaggc ggtctacgag cgcgccagcc gcctggcggc gtcgctgtgg agacggatgc
      361 gtggctgagt tcgtgccggt gccgatcggg cgcctgctgc gccgggcgca ccgggagtac
      421 agccggcagg gggcggtttt cggcctgccg gagcagaagt tctaccgccc gccggccctg
      481 cgcgacgcgg acacggcacc aggcccgagc accctggatc tttccgtccg cgtatcaggc
      541 cagcgggccg gcaacccgat gggcctcgcc gcgggaccgc acacgcagct cgcccagaac
      601 atcgctctcg gatggctcgc gggcgcgcgc gtgttcgaac tcaagaccgt ccaggtgaac
      661 gatcggatca cggtgccgcg cccgtgcatc gacgccgcga caatcggcta caacaccgag
      721 tggtcgcagg agctccggct ggacgagtcg ctgcgcgagt acgtcaaggc gtcgatgctc
      781 atcgaggcgc tggaggcgat gggtatcctc gggcggggca tcggactcga gggccgggag
      841 tcgccgggcg gaccgccccc gcgcgcgacg cccgtcgacg gcggcgtcgt cttcgatgcg
      901 agcgtcgggt atgacctcga gggcatccgg agccccgcga tggcgtcgtg gctggaatcg
      961 atccggagcg ccggcgcgat cgtcgatgac ctcaggcggg agatcgcggc cgcgttcccg
     1021 gggcttcgcg acttcccctt caagacgcgc atcgcggccg gcgtcaccgt gtccaccttc
     1081 caccacacgc cgccgggcga gatcgagcgg atcggcgagt tcctcatcgg cgatctgggc
     1141 ttccatacga tgatcaaggt gagcccgccg atgctgggac gcgagcgcct cgaggccatc
     1201 ctgcacggcg ccctcggata cgaagaagtg gcggtcgatc cctccgtcta cgagcgatgc
     1261 ctgagcttcg aggaggccgc cgggatcatg cgccggctgc agccgctcgc ggcccggcgc
     1321 ggcgtcaccg ccggcgtgaa gtgcggcaac acgctcgaag tcgttggcgc ggccacgtcg
     1381 ctgacggcgc gggtgcagta cctgtccggg cagccgctcc acgcgctgca cgccgcgctg
     1441 gcagccgagt ggcgcgaggc gttcggaccg gacctgccgc tggcgttcgc cggcggcgtg
     1501 gacgccggca acgccgccga ctgcgtggcg gcgggcctcg cgccggtgac cgtctgcacg
     1561 gacctgctgc gcgccggcgg gtacgggcgg ctgccgcgct atctcgtccg cctcgacaca
     1621 cgcatgcggg cggtgg
//
LOCUS       DAOSIF010000627         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_64972_length_1633_cov_3.441065, whole genome shotgun sequence.
ACCESSION   DAOSIF010000627 DAOSIF010000000
VERSION     DAOSIF010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64972_length_1633_cov_3.441065"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLT35_14560"
     CDS             <1..537
                     /locus_tag="PLT35_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG30495.1"
                     /translation="IQARPLGTADAVASASDVVGERPCLVLNSDNYYPVEAYRALVAM
                     DGPGLVAFERDAMARLGNVPAERAGQLAVVRIGSDGHLLDVIEKPDEATLASLGSEVY
                     LSMNCWRFDRTVLDACGRVAPSARGERELPDAVRLAQREGGTTFRVVRLKAGVLDLSS
                     RSDVAGVARHLAGVEVRL"
     gene            537..>1633
                     /locus_tag="PLT35_14565"
     CDS             537..>1633
                     /locus_tag="PLT35_14565"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOG30496.1"
                     /translation="MVPAAATLASSGLSPACASAKETLFARAAGALPPRPARSRRAAF
                     FVPGRIEVLGKHTDYAGGRSLLCAASHGFCLVGQPRADSRVRVISAATGSEAEWVIGP
                     DLQPVLGDWSNYPATVVRRLARNFPEMTAGADLAFDSDLPPASGMSSSSAFMIAVFLA
                     LADLNGLGQSPAYREAISSREALAAYLATIENGRSFGALAGDRGVGTFGGSEDHTAIL
                     CCKAGTLSQYRFGPVTFERDLAVAPGLAFLVAFSGVLAEKTAAAMATYNRASLAAARI
                     LAIWQQASGCSAPTLESAAAASAGAPDDIRRAIVEAGDDEFGTGCLLERFEQFLLESR
                     AIVPDAGDALIRGDLARFGALVDRSQEAAER"
BASE COUNT          206 a          587 c          601 g          239 t
ORIGIN      
        1 atccaggcgc ggccgctcgg cacggccgac gccgtagcgt cggcgtcgga cgtcgtcggc
       61 gagcggccgt gcctcgtgct caactcggac aattactacc ccgtcgaggc ctaccgcgcg
      121 ctggtcgcga tggatggccc cggcctggtg gccttcgagc gggacgccat ggcccgcctc
      181 ggcaacgttc ccgcggagcg ggccggccag ctcgcggtgg tccgcatcgg gagcgacggc
      241 cacctcctcg acgtcatcga gaagccggac gaggcgacgc tggcgtcgct cggcagcgag
      301 gtgtacttga gcatgaactg ctggcgcttc gaccgcaccg tccttgacgc ctgcggccgc
      361 gtggcgccgt cggccagggg cgagcgggaa ctgccggacg cagtgcggct ggcccagcgg
      421 gaggggggca cgacctttcg cgtcgtccgt ttgaaggccg gggtcctgga cctgtcgagc
      481 cggagcgacg tcgccggcgt agcccggcac ctggccggtg tcgaggtgcg cctgtgatgg
      541 tgccggccgc ggcaacactc gcttcgtcgg gcctgtctcc ggcatgcgcc tcggccaagg
      601 agacgctgtt cgcccgcgcc gccggcgcgc tgccgcctcg gcccgcccgg tcccgccgcg
      661 ccgcgttctt cgtacccgga cggatcgaag tcctgggaaa gcataccgac tacgccggcg
      721 ggcgcagcct gctgtgcgcc gcgagccacg gcttctgcct cgtggggcag cccagggcgg
      781 actcccgcgt gcgggtgatc agcgcggcga ccgggtctga ggcggagtgg gtgatcgggc
      841 ccgacctcca gcccgtgctg ggggactgga gcaactaccc ggccaccgtg gtccgccggc
      901 tcgcgcggaa cttcccggag atgaccgccg gggccgatct cgccttcgac agcgacctgc
      961 cgcccgcatc gggcatgtcg agctcgagcg cgttcatgat cgccgtgttc ctcgcgttgg
     1021 cggacctgaa cggcctcgga cagtcgcccg cgtatcgcga ggccatctcg tcgcgcgagg
     1081 ccctggccgc ctatctcgcg acgatcgaga acggccgcag cttcggcgcc ctggcgggcg
     1141 accgcggggt cggaaccttc ggcggcagcg aggatcacac ggcgatcttg tgctgcaagg
     1201 ccggcacgct cagccagtac cgcttcggcc cggtgacctt cgagcgcgac ctagccgtcg
     1261 cacccgggct ggccttcctc gtggcattca gcggcgtgct ggcggagaag accgcggccg
     1321 cgatggccac ctacaaccgc gcgtcgctcg cggcagcccg gatcctggcg atctggcagc
     1381 aggcgagcgg atgctcggcg ccgaccctcg agtccgccgc ggcggcctca gccggcgcgc
     1441 cggatgacat ccggcgggcg atcgtcgaag ccggtgacga cgagttcggc acgggctgcc
     1501 tgctggagcg cttcgagcag ttcctgctcg agagccgtgc gatcgtcccc gacgcgggcg
     1561 acgccttgat ccgaggcgac ctcgcgcgct tcggcgcgct cgtggatcgt tcgcaggaag
     1621 cggcggaacg ctg
//
LOCUS       DAOSIF010000628         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_65273_length_1628_cov_3.083916, whole genome shotgun sequence.
ACCESSION   DAOSIF010000628 DAOSIF010000000
VERSION     DAOSIF010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65273_length_1628_cov_3.083916"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLT35_14570"
     CDS             <1..527
                     /locus_tag="PLT35_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30497.1"
                     /translation="WVGVVVEGSFWAGVVATGDVPAARRRPSVQTTLTETEFNAYLRF
                     TAKDAIPAGIVNPYVFALGNGRLAGEATVDLDAVRLSRPRGVFDFAQLLRGQVAVTVV
                     CVLRSERGAARLDLESATAGGFALPKPLLQELVSFYTRSASNPAGVSLDAPFALPSGI
                     REIAVQVRKAVITQ"
     gene            524..>1628
                     /locus_tag="PLT35_14575"
     CDS             524..>1628
                     /locus_tag="PLT35_14575"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG30498.1"
                     /translation="MTLDTPLASLKGVGPRRAADLAAAGLHAVGDLLARFPLRYDDRA
                     RFRDAASVRDGQWVTVAGSVASCGLRLTRRRGFKLFEAVVRDQSGPLRAVWLNQPFMA
                     SVISRGAEVILYGRAERRADGSLQLTNPQYELPARGERGSLHTGRIVPIYEKAGCVTC
                     RMQRALVRDALDRLPADAPDLLPEWLRGELGLPGWREAVEAAHFPPEGAPLEALNAFR
                     TPAQIRLIFEECLLFQVGLALRRRRADAERKPVVPAVNDRVRAAARAVLPFRLTAGQR
                     QALKEIVADMAQPRPMQRLLQGDVGAGKTIVALLAAVVAMENGLQVAFMAPTEVLAEQ
                     HFLTIRRHLEPSRFTVELLTGSTPAVERRRIVEG"
BASE COUNT          206 a          574 c          601 g          247 t
ORIGIN      
        1 gatgggtcgg cgtggtcgtc gagggttcgt tctgggccgg cgtcgtcgcc acgggcgacg
       61 tgcctgcggc gcgccgccgg ccgagcgtgc agacgacgct caccgagacc gaattcaacg
      121 cgtacctccg cttcacggcg aaggatgcga ttcccgccgg catcgtcaat ccgtacgtgt
      181 tcgccctggg caatggccgg ctcgcgggcg aggcgacggt ggatctcgac gccgtccgtc
      241 tgtcgcggcc gcgcggcgtc ttcgatttcg cgcagctgct tcgcggacag gtcgcggtga
      301 cggttgtctg cgtgctccgg tcggagagag gcgcggcccg gctcgatctg gaatcggcca
      361 ctgccggcgg cttcgcgctc cccaagccgc tgctgcagga actcgtgtcg ttctacacgc
      421 gcagcgcgtc gaaccccgct ggggtgagcc tcgacgcgcc attcgccctt ccctccggca
      481 ttcgcgagat cgccgtgcag gtgcggaagg ccgtcattac ccagtgacgc tggacacgcc
      541 gcttgcctcg ctcaagggcg tcggcccgag gcgggcagcc gacttggccg cggcaggcct
      601 gcacgcggtg ggcgacctgc tggcgcggtt tccgttgcgg tacgacgacc gcgcgcggtt
      661 ccgggacgcc gcgtcggtgc gcgacgggca gtgggtcacc gtcgccgggt ccgtggcgtc
      721 gtgcgggctc aggctgacgc ggaggcgcgg cttcaagctg ttcgaggctg tcgtgcgcga
      781 ccagagcggc ccgctgcggg ccgtctggct gaaccagccg ttcatggcca gcgtgatcag
      841 ccgcggcgcg gaggtcatcc tctacgggag ggccgagcgc cgcgcggacg gaagcctgca
      901 actcaccaac ccgcagtacg aactgcctgc ccgcggcgag cggggctcgc tccatacggg
      961 gcggatcgtg ccgatctacg agaaggccgg ctgcgtcacc tgccggatgc agcgggcgct
     1021 cgtgcgcgac gcgctggatc gccttccggc cgatgcgccc gacctgctgc ccgagtggct
     1081 gcgcggcgaa ctcggactgc ccggctggcg ggaggccgtg gaggctgcgc acttcccgcc
     1141 ggaaggggcg ccgctcgagg cgctcaacgc gtttcgcact cccgcgcaga tccgactgat
     1201 cttcgaggag tgcctcctgt tccaggtcgg gctcgccctg cgcaggcgcc gggccgacgc
     1261 cgaacggaag ccggtcgtgc cggccgtcaa cgaccgcgtg cgggcggcgg cgcgggcggt
     1321 gctgccgttc aggctgaccg caggccagcg ccaggcgctg aaggagatcg tcgcggacat
     1381 ggcgcagccc cggcccatgc agcgcctcct gcagggcgat gttggcgccg gcaagaccat
     1441 cgtcgcgctc ctcgccgccg tcgtggccat ggagaacggc ctccaggtgg ccttcatggc
     1501 gcccaccgag gtgctcgccg agcagcactt cctcacaatc cggcggcacc tcgagccgtc
     1561 gaggttcacc gtggagctgc tgacgggctc gaccccggcc gtcgagcgca ggcgcatcgt
     1621 cgagggcc
//
LOCUS       DAOSIF010000629         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_65850_length_1618_cov_4.191299, whole genome shotgun sequence.
ACCESSION   DAOSIF010000629 DAOSIF010000000
VERSION     DAOSIF010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65850_length_1618_cov_4.191299"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1618
                     /locus_tag="PLT35_14580"
     CDS             <1..>1618
                     /locus_tag="PLT35_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG30499.1"
                     /translation="TFVERGMVYKGKKPVHWCIHCRTALAEAEVEYEDHASPSIYVEF
                     PLGAEGSAALASRFPGLEGRRVSALIWTTTPWTIPANLALAFHPDAVYAVCPVDGRAV
                     IVAEALVEGLSAALGRPFEEPVARVPGTAFEGLKFRHPLYERDSVGVLADYVTLDTGT
                     GIVHTAPGHGSEDFQTGVKYGLDIYAPVAADGRFTSDVERVAGLTVFEANPVVEAALG
                     EAGALWKTESFSHAYPHCWRCRNPVIFLATSQWFIAMDDTGLRRQAIAETRRVRWVPE
                     WGGDRMRSMVETRPDWCISRQRTWGVPIPALTCSSCGEAVLTAGLVERAAAVFERDGA
                     EAWYERPVADFVPEGLACPSCGGTAFERERDILDVWFDSGSSHEAILVVHPELHWPPD
                     LYLEGTDQYRGWFQSSMLVALGTRGRAPYDQVLTHGMVVSEDGTKMSKSLGNDVPPDE
                     VIRKTGAEILRLWVASVDFREEVRFGSEILARVVEAYRKLRNTLRILAANLSDFDPAA
                     HMVPPQAMDEVDRYVMARYGEFASRVLTAYD"
BASE COUNT          222 a          560 c          591 g          245 t
ORIGIN      
        1 acgttcgtcg agcgcggcat ggtgtacaag ggcaagaagc ccgtgcactg gtgcatccac
       61 tgccgcacgg cgctcgccga ggcggaagtc gagtacgagg accacgcctc cccatcgatc
      121 tacgtcgagt tcccgctggg ggccgagggg tcggccgcgc tcgcctcgcg gttccccgga
      181 ctcgaagggc gtcgggtgtc ggcgctcatc tggacaacga cgccctggac cattccggcc
      241 aacctggcgc tcgcgttcca cccggacgcc gtctacgccg tctgtcccgt cgacggcagg
      301 gccgtgatcg tggccgaagc cctcgttgag ggcctgtccg cggcgctcgg ccggccgttc
      361 gaggagccgg tggcgcgcgt gccgggcacg gcgttcgagg ggctgaagtt ccgccatccg
      421 ctctatgagc gggactcggt gggcgtgctg gccgactacg tcacgctcga cacgggcacc
      481 gggatcgtgc acaccgcccc gggacacggc agcgaggact tccagaccgg cgtgaagtac
      541 gggctcgaca tctacgctcc ggtggccgcc gacgggcggt tcaccagcga cgtggagcgc
      601 gtcgccggcc tcaccgtctt cgaagcgaac ccggtcgtgg aggccgccct cggcgaggcc
      661 ggcgcgctct ggaagacgga gtcgttctcg catgcgtacc cgcactgctg gcgctgccgc
      721 aaccccgtga tcttcctcgc cacgtcgcag tggttcatcg ccatggacga caccgggctc
      781 cggcgccagg ccattgccga gacgcgccgc gtccgatggg tgcccgaatg gggcggcgat
      841 cggatgcgga gcatggtgga aacccgcccg gactggtgca tctcccgcca gcgtacgtgg
      901 ggcgtgccca tcccggcgct cacgtgctcc tcgtgcggcg aggccgtgct gaccgccggg
      961 ctcgtggagc gggcggcggc cgtcttcgag cgggacggcg cggaggcctg gtacgagcgc
     1021 cccgtcgccg acttcgtgcc cgaggggctc gcctgcccgt cgtgcggcgg caccgcgttc
     1081 gagcgcgagc gggacatcct ggacgtgtgg ttcgactccg gctcgagcca cgaggcgatc
     1141 ctcgtggttc acccggagct gcactggccg ccggacctgt atctcgaggg caccgaccag
     1201 taccgcggct ggttccagag ctcgatgctg gtcgccctcg ggacgcgcgg gcgggcgccg
     1261 tacgatcagg tgctgacgca cgggatggtg gtctccgagg acggcacgaa gatgtccaag
     1321 tccctcggca acgacgtgcc gcccgacgag gtgatccgga agaccggggc ggagatcctg
     1381 cgcctctggg tcgccagcgt ggacttccgc gaggaggtgc gcttcgggag cgagatcctg
     1441 gcgcgggtcg tggaggccta ccggaagctg cggaatacgc tccggatcct ggcggccaac
     1501 ctgtccgact tcgaccccgc cgcgcacatg gtgccgcccc aggcgatgga cgaggtggac
     1561 cgctacgtga tggcccgcta cggggagttc gcctcccgcg tcctgacggc gtacgacg
//
LOCUS       DAOSIF010000630         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_66022_length_1615_cov_2.972436, whole genome shotgun sequence.
ACCESSION   DAOSIF010000630 DAOSIF010000000
VERSION     DAOSIF010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66022_length_1615_cov_2.972436"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1615
                     /locus_tag="PLT35_14585"
     CDS             <1..>1615
                     /locus_tag="PLT35_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30500.1"
                     /translation="YTHFKYPTQGRPDFVANVTPSLVAGTKLDLNNLDKVGQLSVPTP
                     VAGQDAYNAFVAPIIERYGYLSNGARTGGGTVGYYTQFDKDDFFRDAAQVAYNLTVGS
                     SVRHDIHAGFQWYIDSEDLKRSSNGWGSISVPGGRLASAGVGGVNAYYQAIYLQQTTG
                     QVPKIHSEYVSYNIELNDTIAWKNLSLNLGVLASKDVLYGQGLKNDASTLSGFSLAPG
                     NKYKMYTIPFKKMIQPRVGLTWAYNGTDIVYASFATYNPAASSLPRAAAWDRNIAGAQ
                     VNAYFDQAGNYYGNTMTLSSSGKLFADGLTPRTITEYLAGTSKQFTQALTGRAYFRYR
                     KGAHFWEDTNNNGRILFNPPAGIPRELYVPDLSAKLAQIGSGSTYVIAELDGAYTKYY
                     EATVESEWRPKNAYLRGSFTWSRYVGNFDQDNSTTDNDQNIFIGSSNIGDGAGRQLWD
                     FRDGTLRGDRPASLKLYGYYQLPWHASLGAYGILQSGQPWEKWSYEPYILLTTSTSDT
                     SRYAEKAGSRRTPTHYQVDLNYTQNLRIYR"
BASE COUNT          325 a          556 c          496 g          238 t
ORIGIN      
        1 tacacccact tcaagtaccc gacgcagggc cggccggact tcgtggccaa cgtcacgccg
       61 agcctggtcg cgggcaccaa gctcgacctc aacaacctgg acaaggtcgg ccagctctcg
      121 gtgccgaccc cggtcgcagg ccaggatgcc tacaacgcgt tcgtcgcgcc gatcatcgag
      181 cgctacgggt acctctccaa cggcgcgcgg accggcggcg ggacggtcgg ctactacacc
      241 caattcgaca aggacgactt cttccgagac gcggcccagg tcgcctacaa ccttacggtg
      301 ggcagcagcg tgcgccacga catccatgcc ggattccagt ggtacatcga ctcggaggac
      361 ctgaagcgca gctcgaacgg atgggggtca atctcggtcc caggcgggcg cctcgcgagc
      421 gcgggggtcg gcggtgtgaa cgcctactac caggcgatct acctccagca gaccacgggc
      481 caggtcccca agattcactc ggagtacgtg tcctacaaca tcgagctgaa cgacacgatc
      541 gcgtggaaga acctgtcgct caacctcggg gtgctggcca gcaaggatgt gctgtacggc
      601 caggggttga agaacgacgc gtccacgctg tccggcttca gcctcgcgcc gggcaacaag
      661 tacaagatgt acaccatccc cttcaagaag atgatccagc cgcgcgtggg cctcacgtgg
      721 gcctacaacg ggacggacat cgtctatgcg agcttcgcga cgtacaaccc cgcggccagc
      781 tcgctgcccc gtgcggcggc atgggaccgc aacatcgccg gcgcgcaggt caacgcctac
      841 ttcgatcagg ccggcaatta ctacggcaac acgatgacgc tgtcgtcctc cggcaagctg
      901 ttcgcggacg gcctgacgcc acgcacgatc acggagtacc tggcgggcac gtccaagcag
      961 ttcacccagg cgttgaccgg tcgcgcgtac ttccgctacc gcaagggagc ccacttctgg
     1021 gaggacacca acaacaacgg gcggatcctg ttcaacccgc ccgcggggat cccgcgcgag
     1081 ctgtacgtcc cggatctctc ggcgaagctg gcccagatcg gcagcggctc gacctacgtc
     1141 atcgccgagc tcgacggcgc ctatacgaag tactacgaag cgacggtgga gtcggagtgg
     1201 cggccgaaga acgcgtacct gcgcgggtcg ttcacgtgga gccggtacgt gggcaacttc
     1261 gaccaggaca attcgacgac cgacaacgac cagaacatct tcatcggatc gtcgaacatc
     1321 ggcgacggtg cgggccgcca gttgtgggac ttccgcgacg gcacgctgcg cggcgaccgc
     1381 ccggcgtcgc tcaagctgta cggctactac cagctcccgt ggcacgcaag cctcggcgcc
     1441 tacggcatcc tgcagtccgg ccagccgtgg gagaagtgga gctacgaacc gtacattctg
     1501 ctgacgacga gcaccagcga cacgtcgcgc tacgccgaga aagccggctc gcggcgcacc
     1561 ccgacgcact accaggtgga cctcaattac acgcagaacc tgcgcatcta ccgcc
//
LOCUS       DAOSIF010000631         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_66619_length_1604_cov_5.238218, whole genome shotgun sequence.
ACCESSION   DAOSIF010000631 DAOSIF010000000
VERSION     DAOSIF010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66619_length_1604_cov_5.238218"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..>1604)
                     /locus_tag="PLT35_14590"
     CDS             complement(337..>1604)
                     /locus_tag="PLT35_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG30501.1"
                     /translation="VLSRQSVLHLKGSERRIGEIARDLGVDGVVEGAVLHDGDRARVT
                     AKLVLAEPERHAWAHTYDCDLSAVLTTQRDLARAIAGCVATALRPADAAAPRQAAAQP
                     VARDIAELFLNARVEFEKMSAEGIGKALAGFREIALRAPDFAPGLVWYSACLYMLGYW
                     GHAPAGEVYPSAKRLALQALAIDDSLARAHLVLAWMNLLLDWDLSAAMLEVRRAIELG
                     PSESDAHAFHSTLLCFAGSKSESAEAVEYMLKLNPAPLMPNQYAAWMFLHLGQHARAE
                     AQARRTIGRFPDSLQPYFVLGWAAWRQGRAQEAVASLETALAHSREALSLVYLGHMYA
                     QLGRTDEARKLLRELEQRRARGQAPPTAFAAIHAGLGEADAAFEWLETAYRLRDGHLF
                     WLAGDPGLDPLHADARFADLLRRIGVAPA"
BASE COUNT          232 a          564 c          583 g          225 t
ORIGIN      
        1 atgccgtcgg tccattccag ctcgccgccg gacatctcgc ggacccgctt caggatcgcg
       61 tagccgtagc tgtctccgtc ggccaggatg gcgaggacga gggccgtgga cgaggccgcg
      121 acgaggtcct tgttgatgct catcggaccg gcgccctccg ctgccggccg tgcgcgccac
      181 cggagcgggg cggcccggct cggctccgaa gggggctgga gggcgaatac atagaagcag
      241 tatacataga atcgctaggt atgtgagggc ggcggcacac gccagccagc gtcggcagcc
      301 tcaagcgcct ctcgtgctcc tgccgatgcc gcggcgtcag gccggcgcga cgccgatgcg
      361 gcggaggaga tcggcgaagc gggcgtcggc atgcagggga tcgaggcccg ggtctcccgc
      421 gagccagaac aggtggccgt ccctcaggcg gtacgccgtt tcgagccact cgaacgccgc
      481 atcggcctcg ccgagtcccg cgtggatggc cgcgaaggcg gtcgggggcg cctggccccg
      541 cgcgcggcgc tgctcgagtt ctcgcaggag cttcctggct tcgtctgtcc ggccgagctg
      601 cgcgtacatg tgccccagat agaccagcga cagggcttcg cgcgagtggg cgagggccgt
      661 ttccagcgac gcgacggcct cttgcgcgcg gccctggcgc caggcggccc acccgaggac
      721 gaagtacggc tggagggagt ccgggaagcg tccgatcgtc cgcctggcct gcgcctcggc
      781 ccgcgcgtgc tggccgagat gcaggaacat ccacgccgcg tactggttcg gcatgagcgg
      841 cgcgggattc agcttcagca tgtactcgac ggcctcggcc gactcggatt tcgagccggc
      901 gaagcacaac agcgtcgaat ggaacgcgtg cgcgtccgac tcgctggggc ccagttcgat
      961 ggcccgccgc acctcaagca tcgccgcgga gaggtcccag tcgagaagca ggttcatcca
     1021 cgccaggacc aggtgggcgc gggccaggct gtcgtcgatg gccagggcct gcagcgccag
     1081 gcgtttggcg ctggggtaca cctcgcctgc cggagcatgc ccccagtagc ccagcatgta
     1141 caggcaggcg gagtaccaga cgagcccggg cgcaaagtcc ggcgccctga gcgcgatctc
     1201 gcggaagccc gcgagcgcct tcccgatccc ctcggcgctc atcttctcga actcgacgcg
     1261 cgcgttcagg aacagctcgg cgatgtcccg cgccaccggt tgcgccgctg cctggcgcgg
     1321 agcggcggcg tcggcgggcc gcagcgccgt cgcgacgcag ccggcgatcg cccgcgccag
     1381 gtcgcgctgg gtggtcagca cggcggacag gtcgcagtcg taggtgtgcg cccaggcgtg
     1441 gcgttccggc tctgcgagca ccagcttcgc cgtgacgcgg gcgcgatcgc catcgtgcag
     1501 cacggccccc tccacgacgc cgtcgacgcc gaggtcgcgg gcgatctcgc cgatcctgcg
     1561 ctcgctcccc ttcaggtgga gcacggactg gcgcgacagc accc
//
LOCUS       DAOSIF010000632         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_66922_length_1599_cov_3.514896, whole genome shotgun sequence.
ACCESSION   DAOSIF010000632 DAOSIF010000000
VERSION     DAOSIF010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66922_length_1599_cov_3.514896"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLT35_14595"
     CDS             complement(<1..214)
                     /locus_tag="PLT35_14595"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOG30502.1"
                     /translation="MATSDAGSSRKTYVVDTSILVSAPDALQNLTSHNTVLVPFPVLQ
                     ELDRKRTSANGVGYTARNTIKFLDEIQ"
     gene            478..>1599
                     /locus_tag="PLT35_14600"
     CDS             478..>1599
                     /locus_tag="PLT35_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOG30503.1"
                     /translation="MDTVLLARWQFAITSVYHFFFVPLTLGLVWLVAGMHTAWVVTGR
                     DVWKRLTKFWGRLFLINFAMGVATGIVMEFQFGMNWAEYSRFVGDVFGAPLAIEALLA
                     FFLESTFLGLWVFGWDRLPKKVHLACIWVVAVGSNLSALWILIANSFMQQPVGYVINN
                     GRAEMRDFAALVFNPHVLVQFPHVITGSMALAGFFVLGISAWQIRRSPDEDNRDAFRR
                     SFRIGAVYALVASLAVAVVGHSQAQHMVRVQPMKMAAAEALWESADPAPLSLFTWGNE
                     PERRDVWAIKAPSLLSFLAYNRFHGEVKGIKNVQADYERRYGPGDYAPPIMWTYWTFR
                     AMVGAGTLMVLLAAWAVLAARRNRIDASPRLLALLVPAIA"
BASE COUNT          216 a          538 c          568 g          277 t
ORIGIN      
        1 tctggatctc gtccaggaac ttgatcgtgt tgcgcgcggt gtagcccacg ccgttcgcgg
       61 aggtccgctt gcgatccagc tcctggagga ccgggaacgg cacgaggacg gtgttgtgcg
      121 acgtgaggtt ctggagcgcg tccggagcag aaacgaggat gctggtgtcg acgacgtagg
      181 tcttccggga tgatcctgcg tccgatgtcg ccatccgggc ctcctggggc cgggacgcgg
      241 cgccgcggcg cggacgcgcc ttcgccgccg gggcgaggac gaccggccgc cgagcccgtt
      301 ccggctctgg acctggaggc tcccgcgctg ggatgcggac gccgcctgga ctgccgtgcg
      361 gacatcataa cgccgaacgc cagcggccgc gcaacaagta tcgcggcgaa cgaggtagac
      421 tacgcgccag cgggccgggc cgccgcccgg ccgcgtgagc ctcgggagga ggttcgcatg
      481 gacaccgtcc tcttggcccg atggcagttc gccatcacgt cggtctatca cttcttcttc
      541 gtcccgctga ccctcgggct cgtctggctg gtcgcgggca tgcacaccgc ctgggtcgtc
      601 acgggccgcg acgtctggaa gcggctgacg aagttctggg gacggctctt cctcatcaac
      661 ttcgccatgg gcgtggcgac cggcatcgtc atggagttcc agttcgggat gaactgggcc
      721 gagtactcgc gcttcgtcgg cgacgtcttc ggcgcccccc ttgccatcga ggcgctgctg
      781 gcgttcttcc tcgagtcgac cttcctcggg ctgtgggtct tcggatggga caggctgccg
      841 aagaaggtcc acctggcgtg catctgggtg gtcgccgtcg gatcgaacct gtcggccctg
      901 tggatcctca tcgccaattc gttcatgcag cagccggtcg ggtacgtcat caacaacggc
      961 cgcgccgaga tgcgggactt cgcggcgctg gtcttcaacc cgcacgtgct ggtgcagttc
     1021 ccgcacgtca tcaccggctc gatggcgctg gccgggttct tcgtgctggg gatcagcgcc
     1081 tggcagatcc gccggtcgcc cgacgaggac aaccgcgacg ccttccggcg gtcgttccgg
     1141 atcggcgccg tctacgccct cgtcgcgtcg ctggcggtgg ctgtcgtcgg ccacagccag
     1201 gcccagcaca tggtccgcgt ccagccgatg aagatggcgg cggccgaagc gctctgggag
     1261 agcgcggacc cggctcccct gtcgctgttc acgtggggca acgagcccga gcggcgcgac
     1321 gtgtgggcga tcaaggcgcc cagcctgctg agcttcctcg cgtacaaccg cttccacggc
     1381 gaggtgaagg gcatcaagaa cgtgcaggcg gactacgagc gccggtacgg gcccggcgac
     1441 tacgcgccgc cgatcatgtg gacgtactgg accttccgcg ccatggtggg cgccgggacg
     1501 ctgatggtgc tgctggcggc ctgggccgtg ctcgcggcgc ggcggaaccg catcgacgcc
     1561 agcccgcggc tgctcgcgct gctcgtgccg gccatcgcg
//
LOCUS       DAOSIF010000633         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_67317_length_1593_cov_4.046164, whole genome shotgun sequence.
ACCESSION   DAOSIF010000633 DAOSIF010000000
VERSION     DAOSIF010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67317_length_1593_cov_4.046164"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLT35_14605"
     CDS             <1..775
                     /locus_tag="PLT35_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30504.1"
                     /translation="INFVTCHDGFTLRDLVSYSRRHNEENGEGNRDGEPNNLSWNCGV
                     EGDTDDAATLALRARQVRNLMATLLLSQGVPMITAGDEIGRTQLGNNNAYCQDNEISW
                     LDWSLTPAQRDLLAFTTRLVRFRSAHPVLRRRTFFQGRGIRGQDVTDILWFDPDGKEM
                     TDAGWNAPHARALGVMLVGDAIPEVDERGEPVRDDTLLILLNAGEHPLAFTLPAVPAR
                     TSWEVEFDTGGGRLAPRSGSAPSAFTLGGASLAVLRLVQ"
     gene            869..>1593
                     /locus_tag="PLT35_14610"
     CDS             869..>1593
                     /locus_tag="PLT35_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30505.1"
                     /translation="MSFRTLGAWLTAGAAFWASLGALDVTSGGGGIVRVAMLPALGQL
                     VACLLLAILLGGVLRFRMAGPAGSVPGQTGPRRASGRGDSFEPLYALGVLVLPYLPWL
                     PDRLPVLRVFAGPGRLLVWLVVAAQVAWSVLGTAGRGRRFVVRMRAWTPARGTLAAGA
                     AAALLFSGTALALAPSGFFPGGDEPHYLVMMQSLLKDGDLRIENNHAQRDYEAFFDGD
                     LPPHAIAPGRNGAMYSVHPVGLPL"
BASE COUNT          209 a          584 c          556 g          244 t
ORIGIN      
        1 catcaacttc gtcacctgcc acgacggctt cacgctgcgc gatctggtga gctactcgcg
       61 ccggcacaac gaggagaacg gcgaaggcaa ccgggacggc gagcccaaca acctgagctg
      121 gaactgcggc gtcgaaggcg acacggacga cgccgccacg ctcgccctgc gcgcgcgcca
      181 ggtccggaat ctcatggcca cgctgctgct ctcccagggc gtgccgatga tcacggccgg
      241 cgacgagatc ggacggacgc agctcggcaa caacaacgct tattgccagg acaacgaaat
      301 cagctggctc gactggtcgc tcacgccggc gcagcgggac ctgctcgcgt tcaccacgcg
      361 gctcgtccgg ttccgctcgg cgcacccggt cctgcgccgg cggacgttct tccagggccg
      421 cggcatccgc ggccaggacg tcaccgacat cctctggttc gatccggacg ggaaggagat
      481 gaccgatgcc ggctggaacg cgccgcacgc ccgcgcgctg ggcgtcatgc tggtcggcga
      541 cgccatcccg gaggtggacg agcgcggcga gccggttcgg gacgacacgc tgctgatcct
      601 gttgaacgcc ggcgaacacc cgctggcctt cacgctgccg gcagtgccgg cgcgaacgtc
      661 ctgggaggtg gagttcgaca ccggcggagg caggctggct ccccggtcgg gatcggcgcc
      721 gtcggccttc acgctcggcg gcgcgtcgct tgccgttctt cgcctggtgc aatgaccgca
      781 aggccgctca ggcggccccg gcccgggaga tctcgagacg atgccgcggc ggcaagatag
      841 aattgacggt cctcagcgga gactcgacgt gagctttcgg acactgggcg cgtggctgac
      901 cgccggcgcc gcgttctggg cctcgctcgg ggcgctcgac gtcacctccg gcggcggcgg
      961 catcgtccgc gtcgccatgc tgcccgccct cggccagctc gtggcgtgcc tcctgctcgc
     1021 gatcctgctc ggcggcgtgc tccgtttcag gatggcggga ccggccgggt ccgtacccgg
     1081 gcagaccggc ccgcggcgtg cgtccgggcg gggcgactcg ttcgaacccc tctacgccct
     1141 cggcgtcctc gtcctgccgt atttgccgtg gcttccagac cggctgcccg tgctgcgcgt
     1201 cttcgcgggc ccgggccgcc ttctcgtctg gctggtcgtc gcggcccagg tcgcctggtc
     1261 cgtcctggga acggcgggcc ggggccgccg cttcgtcgtc cgcatgcgcg cgtggacgcc
     1321 cgccaggggg acgctggcag ccggggcggc ggccgccctg ctcttcagcg gcaccgccct
     1381 cgcactcgcg ccgtccggct tcttccccgg gggcgacgag ccccactacc tcgtcatgat
     1441 gcagagcctg ctgaaggatg gggatctgcg gattgagaac aaccacgcgc agcgggacta
     1501 cgaggcgttc ttcgacgggg acctcccgcc tcacgccatc gcgcccgggc ggaatggcgc
     1561 catgtactcc gtccatcccg tcgggctgcc gct
//
LOCUS       DAOSIF010000634         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_67321_length_1593_cov_3.641092, whole genome shotgun sequence.
ACCESSION   DAOSIF010000634 DAOSIF010000000
VERSION     DAOSIF010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67321_length_1593_cov_3.641092"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1344)
                     /locus_tag="PLT35_14615"
     CDS             complement(160..1344)
                     /locus_tag="PLT35_14615"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG30506.1"
                     /translation="MRRIPLERAALWLLYGTVAALQVSIAAAQTLLALMLVAWIAMLT
                     QGRARPGAPPFFRALAVYAVFTLAATVFSVEPLTSLVDDKQLLLLLTVPAVYEIARGG
                     RARTVLAVIITAGAASAVAGIVQYGMLHYNNLGQRPQGSLGHYMTYSGLLMLVAMAAA
                     ARLLFEARNRTWPALVMPALLVALALTFTRSAWVGASAGIAALLLLKDFRLLGALPVV
                     LAVGIAIAPPQVTHRFYSMFDLNDPTSRDRVAMLREGVRMIAADPLTGMGPNMVERQY
                     ARFRDAEAVERVNPHLHNVPMQIAAERGLPALFAWLWFVGSASAGLWALLRRGRHRVL
                     AAAGLGAVAAMLGAGMFEHNFGDSEFLLLLLVLVTLPFASERPEPADPGAPGARRTEG
                     LS"
     gene            complement(1341..>1593)
                     /locus_tag="PLT35_14620"
     CDS             complement(1341..>1593)
                     /locus_tag="PLT35_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30507.1"
                     /translation="ATTATPMVVIYGPTTPGVWGPWRPRACATEVVELEGLPCRPCDQ
                     RVCEPGDFRCLRRVTPAEVASAAERAIERGRLSRRRGVS"
BASE COUNT          258 a          597 c          563 g          175 t
ORIGIN      
        1 cgtgctcgac caccggcacc ggggcctcgg gcgagatccg cctgacgcgg ccgatgacga
       61 actggtccag catgaggtcg ccgacgacca ggatctcgac cgacgcgaag cgcccgacaa
      121 tctcgcacgc gcgcgcgccc tgcaaggcgg acctcgtcat cacgagagtc cctccgtccg
      181 ccgcgcaccc ggggctccgg gatcggcggg ctccgggcgc tcggacgcga agggcagcgt
      241 cacgagcacg agcagcagca ggaggaactc cgagtcgccg aagttgtgct cgaacatccc
      301 ggcgccgagc atggcggcca ccgcgccgag gccggcggcc gcgagcacgc ggtggcggcc
      361 gcggcgcagg agcgcccaca gccccgcaga ggcgctcccg acgaaccaca gccaggcgaa
      421 gagcgcgggc aggccgcgct cggccgcgat ctgcatcggc acgttgtgca ggtgcggatt
      481 gacgcgctcg acggcctcgg cgtcccggaa ccgcgcgtac tggcgctcca ccatgttcgg
      541 gcccatgccc gtgagcgggt cggcggcgat catccgcacg ccctcgcgca gcatcgccac
      601 ccggtcccgg ctcgtcgggt cgttcaaatc gaacatggag tagaaacgat gggtcacctg
      661 cgggggcgcg atggcgatgc ccacggccag cacgacgggc agcgcgccga gcagacggaa
      721 gtccttcagg agcagcagcg cggcgatgcc cgcgctggcg cccacccacg cgctccgcgt
      781 gaaggtcagc gcgagcgcca ccagcagcgc cggcatcacg agcgcgggcc acgtgcggtt
      841 gcgcgcttca aacagcagcc gcgccgccgc cgccatggcg acgagcatca gcagccccga
      901 atacgtcatg tagtggccga gcgacccctg cggccgctgg ccgaggttgt tgtagtgcag
      961 catcccgtac tggacgatgc cggcgacggc gctggcggcg cccgccgtga tgatcacggc
     1021 cagcaccgtg cgggcccggc cgccgcgggc gatctcgtag accgctggca cggtcagcaa
     1081 caggagcagc tgcttgtcgt cgacgaggct cgtcagcggc tcgaccgaga agaccgtggc
     1141 cgccagcgtg aagacggcgt agaccgccag cgccctgaag aacggcgggg cgccaggccg
     1201 tgcgcggccc tgtgtcagca tcgcgatcca ggcgacgagc atcagcgcga gcagcgtctg
     1261 cgcggcggca atcgacacct gcagcgccgc tacggtcccg tagagcagcc acagcgccgc
     1321 gcgttccagg ggtattcgcc tcatgacaca ccccgcctcc gcgaaagccg cccccgctcg
     1381 atggcgcgct cggccgccga ggcgacctcc gccggcgtga cgcgccgcag gcagcggaag
     1441 tcgcccggct cgcacacccg ctggtcgcac ggccggcagg gaagcccttc cagctcgaca
     1501 acctcggtcg cgcacgcacg cgggcgccag ggcccccaca cgcccggcgt cgtcgggccg
     1561 tagatgacca ccatgggcgt cgcggtcgtc gcc
//
LOCUS       DAOSIF010000635         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_67737_length_1586_cov_3.057479, whole genome shotgun sequence.
ACCESSION   DAOSIF010000635 DAOSIF010000000
VERSION     DAOSIF010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67737_length_1586_cov_3.057479"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLT35_14625"
     CDS             complement(<1..223)
                     /locus_tag="PLT35_14625"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG30508.1"
                     /translation="MLDLTAVDTLDILMVVSEAVPYSKTGGLGDVGGALPGALARLGH
                     RVTVVTPRYRGVESGDEVAAFPRGAWLGSP"
     gene            complement(257..>1586)
                     /locus_tag="PLT35_14630"
     CDS             complement(257..>1586)
                     /locus_tag="PLT35_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG30509.1"
                     /translation="SAVELDLEGYAEAMYPLHHYGWSDLRSLRSGRFKVIDAPRPELY
                     DLESDPNELRDIFQQRRVVGEQMITRLREMEQGFANAPAAPPASDVDPEVRARLAALG
                     YVGSFVASANDPRTGRADPKDKFELFNLLGQARDLAPTKDSFPQVSAILRKVLAADPE
                     VIDAWFSLGNAYFKERRYREAIEQFKRALALKPDYDLAVVNIAAAYRRLGDDDAALAG
                     FEHYLTIDPKDAYVRYQMGEIWLDRGDTARAERYFSEALEMDDHVAPAKNALGAIAFM
                     RGDLDGAAKLAREALAIRRDVRLAHYNLALIAEAKGDMAAAEREYLEELDLHPDAYKA
                     AFNLSRLYARMGESDLEMDALQQALDGNPDFADGHLFLARARLRRGGNLDEAERLARK
                     GLRLKPTAELAPLGHYVLADIYNRRGRPAEAAREVAIARGLEAERGRGRR"
BASE COUNT          227 a          572 c          537 g          250 t
ORIGIN      
        1 cgggcgagcc gagccaggcg ccccgaggga acgccgccac ctcgtcgccg ctctccacgc
       61 cgcgataccg gggcgtcacc accgtgacgc ggtgcccgag gcgtgcgagc gcgcccggga
      121 gggctccgcc cacgtcgccg aggccgccgg tcttggagta cgggacggcc tctgagacca
      181 ccataaggat gtcgagggtg tccacggccg tcaagtctag cattggagcg gccagagcgc
      241 gcggcccggc ccccgctcag cgccggcctc ggccgcgctc ggcttcgagg ccgcgcgcaa
      301 tcgccacctc gcgggcggcc tcggcaggcc tgccccggcg attgtagata tcggccagca
      361 cgtagtgacc gagcggcgcc agctccgccg tcggcttcag gcgcaggcct ttgcgggcga
      421 gccgttcggc ctcgtccagg ttcccgccgc gcctcaggcg cgcccgcgcc agaaacaagt
      481 gcccgtcggc gaagtcagga ttcccgtcga gcgcctgctg cagcgcgtcc atctccagat
      541 cgctctcccc catcctggcg tagagcctcg acaaattgaa cgccgccttg taggcgtcgg
      601 ggtggagatc cagctcctcg aggtactcgc gctcggccgc ggccatgtcc cccttcgctt
      661 ccgcaatgag cgcgaggttg tagtgggcca gcctgacgtc tcgccggatg gccagcgcct
      721 cccgcgcgag cttcgccgcg ccgtccagat cgccgcgcat gaacgcgata gcgccgaggg
      781 cgttcttggc gggcgccacg tggtcgtcca tctccagcgc ctcgctgaag tagcgctcgg
      841 ctcgggccgt gtctccccga tccagccaga tctcgcccat ctggtaccgg acgtacgcgt
      901 ccttcgggtc gatcgtcaga tagtgctcga acccggccag cgcagcgtca tcgtcgccga
      961 gccgccggta ggcggccgcg atgttgacca cggcgaggtc gtaatccggc ttcagggcca
     1021 gcgcgcgctt gaactgctcg atggcctcgc ggtagcggcg ctccttgaaa taggcgttgc
     1081 cgagggaaaa ccaggcatcg atgacttcag gatccgcggc cagcaccttc cggaggatgg
     1141 cgctgacttg ggggaatgag tccttcgtcg gcgccaggtc ccgcgcctgg cccagcaggt
     1201 tgaacagctc gaacttgtcc ttcggatcgg cccggccggt tcgcgggtcg ttcgccgacg
     1261 ccacaaacga gcctacgtac ccgagggcgg ccaggcgcgc gcgcacctcg ggatcgacgt
     1321 ccgaggccgg cggcgccgcc ggcgcattgg cgaaaccctg ttccatctcg cgcaggcggg
     1381 tgatcatctg ctcgccgacc acgcgccgct gctggaagat gtccctcagc tcgttcgggt
     1441 ccgactcgag gtcgtacagt tcggggcgcg gggcgtcgat gaccttgaag cggcccgatc
     1501 gcagcgaccg caggtcgctc cagccgtagt ggtgcagcgg gtacatcgct tcggcgtacc
     1561 cctcgaggtc caactcgacg gccgat
//
LOCUS       DAOSIF010000636         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_67785_length_1585_cov_3.285621, whole genome shotgun sequence.
ACCESSION   DAOSIF010000636 DAOSIF010000000
VERSION     DAOSIF010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67785_length_1585_cov_3.285621"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLT35_14635"
     CDS             complement(<1..205)
                     /locus_tag="PLT35_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30510.1"
                     /translation="MFTRHRSTTPVVLGAVLVVALSASAFAQEPKTAALAQALTQAMD
                     AGKLTAIAAKDPVGQDMYVAAMYF"
     gene            complement(335..769)
                     /locus_tag="PLT35_14640"
     CDS             complement(335..769)
                     /locus_tag="PLT35_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30511.1"
                     /translation="MSGTSTAFLGLIAAAAIVMAVVQVGIAIVVVRLAKRVDAASIRV
                     ERELGPLVERLTQVADNLQRAASLASTQIERVDHALAGAGRRANATLSLVQAAVVGPL
                     REGLAVVAAVRAVLAGVRALRRNGRSAHRATRVDDEHPLFIG"
     gene            complement(766..1014)
                     /locus_tag="PLT35_14645"
     CDS             complement(766..1014)
                     /locus_tag="PLT35_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOG30512.1"
                     /translation="MSDERGGGGFVVAFTLGALVGAVIALLFAPATGEEAREALGEKA
                     KEGAQKTREFLKQQRETLASAVERGREAYHAARDKEPV"
     gene            complement(1140..>1585)
                     /locus_tag="PLT35_14650"
     CDS             complement(1140..>1585)
                     /locus_tag="PLT35_14650"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOG30513.1"
                     /translation="SAGALIQTAGRAARNVHGTVIMYADVVTGSIQAAIAEMARRRAR
                     QLAYNDEHGITPASIVKAIDDVLNSVYERDYVAVPSAREEPPPFRSLAELEARIQQAT
                     RDMKAAAANLEFEKAAGLRDHIKRLRALGLGMGEAPVRSTRARTA"
BASE COUNT          204 a          611 c          539 g          231 t
ORIGIN      
        1 agaagtacat ggcggccacg tacatgtcct gccccaccgg gtccttcgcg gcaatcgcgg
       61 tgagcttgcc ggcatccatc gcctgcgtga gcgcctgcgc cagcgccgcc gtcttcggct
      121 cctgcgcgaa agcggacgcg gacaacgcga cgacgagcac cgcgccgaga acaacggggg
      181 tcgtgctgcg atgacgtgtg aacatggatg tcaccttcgg aagaggtccc cggggactgg
      241 cagttttcta tcatcccggc gcacccgccg tcaatgctcc ggggcgccgg cgcgcgcggc
      301 atgcggcccg ccgatcctgc cggccccgcc tcggctaccc gatgaagagc gggtgctcgt
      361 cgtcaacccg cgtcgcccgg tgcgcggacc gcccgttgcg ccgcagcgcg cggaccccgg
      421 cgagcacggc ccgcacggcc gcgacgacgg ccaggccttc ccgaaggggc cccacgacgg
      481 cggcctgaac gagcgagagc gtcgcgttgg cccttcgccc cgcgccagcc agggcgtgat
      541 ccacgcgctc gatctgggtc gaagcgaggc tggccgcgcg ctggaggttg tcggccacct
      601 gcgtcagccg ttcgaccagc gggccgagct cgcgctccac cctgatcgag gccgcgtcca
      661 cgcgcttcgc caggcgcacg accacgatcg cgatgcccac ctgcacgacg gccatgacga
      721 tcgccgcggc ggcaatcagg ccgagaaacg ccgtcgaggt cccgctcaca ccggctcctt
      781 gtccctggcc gcgtggtacg cctcccgccc gcgctcgacg gcggaggcca gggtctcccg
      841 ctgctgcttc aggaactcgc gggttttctg cgcgccctcc ttggcctttt cgccgagcgc
      901 ctcgcgggcc tcctcgcccg tggccggggc gaagagcagg gcgatgaccg cgcccaccag
      961 cgctccgagc gtgaaggcca ctacgaaacc gccaccgccg cgctcatcag acatgaccgt
     1021 cgctccttcg gcggccccgc gccccgccag gccctcctgg cctggcggcc cgtggccgcc
     1081 cttccatcat acaaccgtcg ggccccggcg gccgttgcgc cgatggaacc ggcgcccggc
     1141 tatgcggtcc gcgcccgcgt cgagcgcacc ggcgcttcgc ccattccgag gcccagggcg
     1201 cgcaagcgct tgatgtgatc gcgcagcccg gcagccttct cgaactcgag gttcgccgcc
     1261 gccgccttca tgtccctcgt ggcctgctgg atgcgggcct cgagctccgc gaggctgcgg
     1321 aacgggggcg gctcctcgcg cgcggacggc accgcgacgt agtcgcgctc gtatacgctg
     1381 ttcagcacgt cgtcgatcgc cttcacgatc gacgcggggg tgatgccgtg ctcgtcgttg
     1441 taggcgagct ggcgtgcgcg ccgtcgggcc atctcggcga tggcggcctg gatgctcccg
     1501 gtcacgacgt cggcgtacat gatcacggtg ccgtgcacgt tccgcgcggc ccggcccgcc
     1561 gtctggatga gcgcgcccgc ggatc
//
LOCUS       DAOSIF010000637         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_68424_length_1575_cov_2.790789, whole genome shotgun sequence.
ACCESSION   DAOSIF010000637 DAOSIF010000000
VERSION     DAOSIF010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68424_length_1575_cov_2.790789"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..>1575)
                     /gene="glgP"
                     /locus_tag="PLT35_14655"
     CDS             complement(216..>1575)
                     /gene="glgP"
                     /locus_tag="PLT35_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOG30514.1"
                     /translation="MFAGLCPRWREAEVPVDHVTNGVHVPSWDSPWTDEVWTRAAGKE
                     RWRGDVSALTADILRLTDEDLWRLRAREREDLVRYARSRLLRKLARHGDLEAARRTVA
                     GVLDPDILTLGFARRFAAYKRPNLLLADPDRLLRLLTNPDRPVQIIVAGKAHPRDEEG
                     KRLIQQWARLAAAPAARARVVFLDDYDMRLATELVQGVDVWINTPRRPWEASGTSGMK
                     VLVNGGLNLSTLDGWWAEAFDAGCGWALGGRSDRPGDDEAEAKQLYALLEDEVVPAFY
                     ERDAEGIPRRWMARIRASMAGLAPQFSSVRMLQEYVQRAYVPAAASYRRRAAGRAGLA
                     RGLDRWSRELQRRWPSIRLGEAHVEEAGGRLVFSIAVELGDVDAGSVRVQLYADAGSG
                     GRPEVHEMNLAERVPGASNAWICRAELETARSPGDFTARVVPLHAEARVPLELPLIAW
                     PR"
BASE COUNT          214 a          608 c          549 g          204 t
ORIGIN      
        1 ggcatggcgc acatggtcga catggcggtg cacttgaaga ccggcggcgc cccgctggat
       61 gtgaaggtcg gcggtctgca ggtcggcgcc ggcctcaggc ttcgcttcta ggacgcccgc
      121 ggccgcgcac caagacaagg tcccccccgc gcgccgcgca acgcacgcct tccctcgcaa
      181 gcggcaacgg cggcccgctc gggcgggcgt tgccgtcagc gcggccaggc gatgagaggc
      241 agttcgagcg gcacgcgcgc ctccgcgtgg aggggcacga cgcgggccgt gaagtctccc
      301 ggcgagcgtg ccgtctcaag ctcggcgcgg cagatccagg cgttcgacgc ccctggcacg
      361 cgttccgcca ggttcatctc gtgcacctcg ggccgcccgc cgctcccggc gtcggcgtac
      421 aactggacgc gcaccgagcc ggcatcgacg tcacccagct cgaccgcaat cgagaacacg
      481 agccgcccgc cggcctcctc cacatgcgcc tcgcccagcc tgatgctcgg ccagcggcgc
      541 tggagctcgc gcgaccaccg gtcgaggccg cgggcgaggc cggcgcggcc ggcggcgcgg
      601 cgccggtacg aggccgccgc cggaacgtag gcgcgctgca cgtactcctg cagcatgcgg
      661 acgctgctga actggggggc cagcccggcc atgctggcgc ggatccgcgc catccaccgc
      721 ctcgggatgc cctcggcgtc gcgctcgtag aacgccggca cgacttcgtc ctccagcagc
      781 gcgtagagct gcttcgcctc ggcctcatcg tcgccgggcc ggtccgaccg gccgccgagc
      841 gcccagccgc agccggcgtc gaacgcctcg gcccaccagc cgtcgagcgt cgagaggttc
      901 aggccgccgt tcacgagcac cttcatgccg ctggtgccgc tcgcctccca cggccgccgc
      961 ggggtattga tccacacgtc gacgccctgc accagttccg tggccagccg catatcgtag
     1021 tcgtcgagga acacgacgcg cgcgcgggct gccggcgccg cggcgagcct cgcccattgc
     1081 tggatcagcc gtttcccctc ctcgtcgcgc ggatgcgcct tgccggcgac gatgatctgg
     1141 accggccggt cggggttcgt gagcagccgc agcagccgat ccggatcggc gagcaggagg
     1201 ttcggccgct tgtaggccgc gaaccgccgg gcgaacccga gcgtcaggat gtccggatcc
     1261 agcacgccgg cgacggtccg ccgcgcggcc tcgagatccc cgtggcgcgc gagcttccgg
     1321 aggagcctcg accgggcata gcgcaccaga tcctcgcgct cgcgggcgcg cagccgccag
     1381 agatcctcgt cggtcagacg gaggatgtcc gccgtgaggg ccgacacgtc cccgcgccac
     1441 cgttccttgc cggcggcgcg cgtccatacc tcgtcggtcc agggcgagtc ccacgacggc
     1501 acgtgcacgc cgttggtgac gtgatcgaca gggacctccg cctcccgcca tcgggggcac
     1561 agcccggcga acatc
//
LOCUS       DAOSIF010000638         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_68486_length_1574_cov_3.518762, whole genome shotgun sequence.
ACCESSION   DAOSIF010000638 DAOSIF010000000
VERSION     DAOSIF010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68486_length_1574_cov_3.518762"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLT35_14660"
     CDS             <1..1033
                     /locus_tag="PLT35_14660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG30515.1"
                     /translation="KDVILRMASMSKAVTTVAAAILLEEGKLLLTDPVSKFLPAYAET
                     TVAATPAPGASGADRYGVVKARRQITIRDLLTHTAGVSYGQGSLAEAQYRDAGIIGWY
                     LSDKKEPIVPLMERLASLPFDAQPGEKYVYGYSTDILGAVVEKASGMPLDRFFEARIF
                     APLKMADSGFYLPPEKRGRLATVYSVTPAGTLERAPEGGVGQGDFVEGPRTCFSGGAG
                     LLSTPMDYARFLQMLLNGGELDGVRVLGPKTIELMTSNHVGSLYANGSMGFGFGFEIV
                     EDVGTAGRYGSPGAYGWGSAYFQRFIVDPKEQMVAVFYAQLIPAGGLDLQERWRDLVY
                     QAIVGPVPR"
     gene            complement(1144..>1574)
                     /locus_tag="PLT35_14665"
     CDS             complement(1144..>1574)
                     /locus_tag="PLT35_14665"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HOG30516.1"
                     /translation="RPNIFGAGADYGKAVNGGAPVWRAGANQTTRLKTEVPLVFGTTT
                     VPAGEYNVLVDLKPGAWTLILSTQPYQQKYAPTNKTDLWGSYNYDPKFDLVRVPMQTV
                     DMNLSAEQFDIGFFDMTQEGGWLGMWWEKTGAKVAFAVAK"
BASE COUNT          237 a          520 c          544 g          273 t
ORIGIN      
        1 gaaggacgtc atcctccgga tggcctccat gtccaaggcc gtcaccaccg tggccgccgc
       61 catcctgctc gaagagggca agctgctgct gaccgacccg gtgtcgaagt tcctgccggc
      121 gtatgccgag actacggtcg cggccacccc tgcgcccggg gcgtccggcg ccgaccgcta
      181 cggcgtcgtg aaggccaggc gccagattac gatccgcgat ctgctcacgc acacggccgg
      241 cgtctcgtac ggtcagggga gcctcgccga ggcgcagtac cgggatgcgg gcatcatcgg
      301 ctggtatctc tcggacaaga aggagccgat cgttcccctc atggagcggc tcgcgtcgct
      361 gccgttcgac gcccagccag gagagaagta cgtctacggc tacagcaccg acattctcgg
      421 cgccgtcgtc gagaaggcgt cgggcatgcc gctcgatcgg ttcttcgagg cgcggatctt
      481 cgcgccgctg aagatggcgg actcgggctt ctatctcccg cccgagaagc gcggacggct
      541 ggccacggtg tactcggtga cgccggcggg cacgctcgag cgcgcgcccg agggcggcgt
      601 cggccagggc gacttcgtgg aggggccgcg gacgtgcttc agcggcgggg cgggcctgct
      661 ttcgacgccg atggactacg cccgcttcct gcagatgctg ctgaacggcg gcgagctcga
      721 cggcgtgcgg gttctcggcc cgaagacgat cgagctgatg acctcgaatc acgtcggctc
      781 gctctatgca aacggctcga tggggttcgg cttcgggttc gaaatcgtcg aggacgtcgg
      841 cacggcgggg cgctacggca gccccggcgc ctacgggtgg ggcagcgcgt acttccagcg
      901 gttcattgtg gacccgaagg agcagatggt cgccgtgttc tacgcgcagc tcattccagc
      961 cggcggcctc gacctgcagg agcgctggcg cgacctggtc taccaggcga ttgtaggccc
     1021 cgtgccgcgg tgaagacccg gaccgagggg ggtcagatct cgatttctgc acctgggacc
     1081 agactcgtcc caggtgcaga aatcgagatc tgaccccgcc gccgtctccg gctcacccgg
     1141 tcgctacttg gcgacggcga acgcgacctt cgcgcccgtc ttctcccacc acatgcccag
     1201 ccagccgcct tcctgcgtca tgtcgaagaa gccgatgtcg aactgctcgg ctgacaggtt
     1261 catgtcaacg gtctgcatgg gcacgcggac gaggtcgaac ttcggatcgt agttgtacga
     1321 tccccacagg tcggttttgt tcgtcggcgc gtatttctgc tggtacggct gcgtcgagag
     1381 gatgagcgtc caggcaccgg gcttgaggtc gacgagcacg ttgtactcgc cggcgggcac
     1441 ggtggtcgtg ccgaacacga ggggcacctc ggtcttgagg cgcgtggtct ggttggcgcc
     1501 ggcccgccag acgggcgcgc ctccgttcac ggcctttccg tagtccgcgc ccgcgccgaa
     1561 gatgttcggg cgcc
//
LOCUS       DAOSIF010000639         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_69139_length_1563_cov_3.788462, whole genome shotgun sequence.
ACCESSION   DAOSIF010000639 DAOSIF010000000
VERSION     DAOSIF010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69139_length_1563_cov_3.788462"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PLT35_14670"
     CDS             complement(<1..1138)
                     /locus_tag="PLT35_14670"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOG30517.1"
                     /translation="MKTPHMRLRRPSAAAGTLAVVCAVAALAGAQAPRPRARDLGVAP
                     GVFPPGPLNAITDVPGVLVGHATVVEGRSVRTGATAILPHPGNLFQEKVPGAVFVGNA
                     FGKLAGSTQVDELGTIETPIVLTNTLSVGAAMQAVVAWTLAQPGNADVRSVNAIVGET
                     NDGGLNDIRNGRVTESQVLAAISGARSGPVEEGSVGAGTGTRCFGWKGGIGTSSRAVP
                     VGGATHALGVLAQSNFGGVLTVDGVPVGRLLGRYAFGPARPPDGAQDWPDGSCMLVVA
                     TDAPLDARDLRRLAARAVFGLARTGSSFSHGSGDFAIAFSTAPGLRVRHGALSLEARA
                     ALPSEAASPLFQAALEATEEAVYNSLFRATTVEGNGLTVDAIPVD"
     gene            complement(1186..>1563)
                     /locus_tag="PLT35_14675"
     CDS             complement(1186..>1563)
                     /locus_tag="PLT35_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30518.1"
                     /translation="LARIGKDYEAKFKTLQTGNDFDASMSAYLRECRLPDLRLETLRS
                     RRLQGRADAAGIELPREWWEHDEEHDLWYLTPDGRRQLKRRLTQERIWAVRQWLQVLT
                     PAVALAAGLVGVIIGLLAMWRWP"
BASE COUNT          213 a          587 c          555 g          208 t
ORIGIN      
        1 ggtccaccgg gatcgcgtcc acggtcaggc cgtttccctc cacggtcgtc gcccggaaga
       61 gcgagttgta gacggcctcc tcggttgcct cgagcgccgc ctgaaagaga ggggacgccg
      121 cctcggacgg gagtgcggcg cgcgcctcaa gcgagagcgc gccgtggcgg acgcgcaggc
      181 ccggagccgt cgagaacgcg atcgcgaaat cgccgctccc gtgggaaaag gacgatcccg
      241 tgcgcgccag gccgaacacc gcccgcgccg cgaggcgcct gagatcccgc gcgtcgagcg
      301 gggcgtccgt ggccacgacc agcatgcacg agccgtcggg ccagtcctgc gccccgtccg
      361 gcgggcgcgc gggcccaaac gcgtaccggc cgaggagcct tcccaccggc acgccgtcga
      421 cggtcagcac gccgccgaaa ttgctctgcg cgagcacgcc cagcgcgtgc gtcgcgccgc
      481 cgaccggcac ggcccgcgac gaggtgccga tgccgccctt ccagccgaag catcgcgtgc
      541 cggtgccggc gccgaccgac ccctcctcga cggggccgct gcgcgcgccg ctgatggcgg
      601 cgagaacctg gctttccgtc acgcgcccgt tgcggatgtc gttcaagccg ccgtcattgg
      661 tttcgccgac gatggcgttg accgaccgga cgtcggcgtt cccgggctgg gcgagcgtcc
      721 aggccacgac ggcctgcatg gccgcgccga cgctcagcgt gttcgtcagc acgatgggcg
      781 tttcgatcgt gccgagctcg tccacctggg tcgagccggc gagcttgccg aaggcgttgc
      841 cgacgaagac cgcgccggga accttctcct ggaacaggtt gcccgggtga ggcaggatcg
      901 ccgtggcgcc cgtgcggacg ctccgcccct cgacgacggt ggcgtggccc acgaggacgc
      961 ccgggacgtc ggtgatcgcg ttgagcgggc ctggagggaa gacgcccggg gcgacgccga
     1021 gatcgcgcgc tctcggcctt ggcgcctgag cgcctgccag cgccgccacc gcgcagacga
     1081 ccgccagcgt tccggccgcc gcgctcggcc gacgcagccg catgtgcggg gtcttcatgt
     1141 cggagctgcg agccccgagc cccgggcccc gggtcctcat cccgctcacg gccaccgcca
     1201 catggcgagc aggccgatga tcacgcccac caggccggcc gccagggcca cggccggcgt
     1261 gaggacctgg agccactgcc tgacggccca gatccgctcc tgcgtgaggc gccgcttcag
     1321 ctggcggcga ccgtcgggcg tcaggtacca caggtcgtgc tcctcgtcgt gctcccacca
     1381 ctcgcgaggc aactcgatgc ccgcggcatc ggcccggccc tgcagcctgc ggctgcgcag
     1441 cgtctcgagg cgcaggtccg gcagccggca ttcccgcaag taggccgaca tggacgcgtc
     1501 gaagtcgttg ccggtctgaa gcgtcttgaa cttcgcttcg tagtcctttc cgatgcgcgc
     1561 cag
//
LOCUS       DAOSIF010000640         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_69253_length_1561_cov_3.490704, whole genome shotgun sequence.
ACCESSION   DAOSIF010000640 DAOSIF010000000
VERSION     DAOSIF010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69253_length_1561_cov_3.490704"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLT35_14680"
     CDS             <1..297
                     /locus_tag="PLT35_14680"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOG30519.1"
                     /translation="SPRGRFEVRSMHKDPVWAKPDWAFVEESQPIPRNPKDRLEYGSL
                     GEYALRFGDGFMIHGTLYERLLGRAVSHGCIRVGREDLRVVWANARVGMPIFIY"
     gene            302..1057
                     /locus_tag="PLT35_14685"
     CDS             302..1057
                     /locus_tag="PLT35_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30520.1"
                     /translation="MSRLLTMTSGAQRAVVLAVTAAGLACAAAACGPKPASRDGDAHA
                     LAELARLQRDNALLARQVELAAGKDFYLLLDPSAARLTLMLKGAILQHFAVRAMRVGH
                     PRVAWVGSRDPRHVQSTVWSAGELDPPRLIDRLVIDAAPPGAASEEEAAPAIPPTPEE
                     LYPVPSRYHIRFAAGLSVEVRPREGDAAVGWWPRLRAAVSEKARDVSSAVRGAERDAI
                     RLRLVLEPKDAQSLYRSLPPSVRLLVLDGRGTP"
     gene            1249..>1561
                     /locus_tag="PLT35_14690"
     CDS             1249..>1561
                     /locus_tag="PLT35_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30521.1"
                     /translation="MAPSDRGVERPASPPRLLDFARAFRGAARAVAFYPPSHQKVAEA
                     VHQLMAAARAATSDGAVCLTVLPHGLMAGGAALDPRESAFSDLAAICHRHGIGAVILD
                     GR"
BASE COUNT          201 a          561 c          573 g          226 t
ORIGIN      
        1 tcgccgcgag ggcgcttcga ggtccgatcg atgcacaaag accccgtgtg ggccaagccg
       61 gactgggcgt tcgtcgagga gtcgcagccg attccgcgga acccgaagga ccggctggag
      121 tacgggtcgc tcggcgagta cgcgctccgg ttcggcgacg gctttatgat tcacggaacc
      181 ctgtacgagc ggctgctggg acgggcggtc tcgcatgggt gcatccgcgt cggccgagag
      241 gacctgcgcg tggtctgggc gaacgcccgt gtgggcatgc cgatcttcat atactgaacc
      301 gatgagcagg ctcctcacca tgaccagcgg cgcgcagcga gcagtcgttc tggcagtcac
      361 ggcggccggc ctcgcgtgcg cggccgcggc gtgcgggccg aagcccgcct cccgagacgg
      421 cgacgcgcac gccctggcgg aactggcccg gctgcagcgg gacaacgcgc tcctcgcgcg
      481 ccaagttgaa ctggccgccg ggaaggactt ctacctgctg ctcgacccgt cagccgcgcg
      541 gctgacgctc atgctcaagg gcgccatcct gcagcacttc gccgtcaggg cgatgcgcgt
      601 cggccatccc agggtggcct gggtgggatc gagagacccc cgtcacgtgc agagcaccgt
      661 ctggtcggct ggggaactcg atccgcctcg gctcatcgac cgtctcgtga tcgacgccgc
      721 gcctccgggc gcggcctcgg aggaagaggc ggccccggcc atcccgccga cgccggagga
      781 gctgtatccc gtgccctccc gctaccacat caggttcgcc gcgggcctct cggtggaggt
      841 ccggccacgc gagggcgacg cggccgtcgg ctggtggccc cgcctgcgcg cggcggtgtc
      901 cgagaaggcc cgcgacgtat cgtcggcggt caggggcgcc gagcgcgacg cgatccggct
      961 gcgcctcgtg ctcgaaccca aggacgcgca gtcgctgtac cggtcgctgc cgccatcggt
     1021 gcggcttctc gtgctggacg gccgcggcac gccctgaggg gcctggtcgc cgggcatccg
     1081 cggctggggg ccatgtcccg ccgccgggcg tccgcgcagg gccgcggcgc acctctcttc
     1141 agtccgcagg acttcggacc gatagatcgg cgaacggtgt gatcggcgcc gggcacggcc
     1201 cgtgccgggc ccgggacgcc ggactgcaac ctgggatccg tgttggcgat ggcaccatcc
     1261 gatcggggcg tcgagcggcc cgccagcccg ccgcgcctcc tggacttcgc gcgggcgttt
     1321 cgcggcgccg ctcgggccgt ggccttttac ccgccgtccc accagaaggt ggcggaagcg
     1381 gtacaccagc tgatggcggc cgcgcgggca gccacaagcg acggtgcggt ctgcctcacg
     1441 gttctgcccc acggcctcat ggccggcggt gcggcgctcg acccgcgtga gagcgcgttc
     1501 tccgatctcg cggccatctg ccaccggcac gggatcggcg cggtgatcct cgacggccgc
     1561 g
//
LOCUS       DAOSIF010000641         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_69379_length_1559_cov_3.083777, whole genome shotgun sequence.
ACCESSION   DAOSIF010000641 DAOSIF010000000
VERSION     DAOSIF010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69379_length_1559_cov_3.083777"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PLT35_14695"
     CDS             <1..1154
                     /locus_tag="PLT35_14695"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30522.1"
                     /translation="YYEQLQYAGDTRIQALVSLYASGDARLMKNAIAHLDDSRTAEGA
                     TMSRAPTRQQQYIPPFSLWWIGMLHDYYMYVDDEAFVRERLGGVRAVLQFFGRHQKPN
                     GSLGRLPWWNYVDWTPEWPGGVPPSQGNGSIAVLDLQLLLAYDWAAHLEDALGSKAVA
                     SEYRASAERLRQAIRPLYWDEGRRLYADTEAHDRYSQHANVLAVLAGVSAGAEARDLV
                     ARVIEDATITRCSYYFRHYLHSAVNLVGEGDRYLSLLADWEGMLGRGLTTFAERYDAP
                     GDPSRSDCHAWSASPNFEIFRTVLGIDTAAAGFAAVRVRPFLGALGRASGSIPHPKGE
                     VSVALVREGRGLRAEIVLPEGVGGEFSWQGVHRPLAPGANRFTAQPAAK"
     gene            complement(1355..>1559)
                     /locus_tag="PLT35_14700"
     CDS             complement(1355..>1559)
                     /locus_tag="PLT35_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30523.1"
                     /translation="ASMAVTIIGGQLLCLLLTLLVTPVVYSYFDDLREMKITDLLRAL
                     ANRVRGRKEVEAAVPGAARTPVR"
BASE COUNT          216 a          544 c          589 g          210 t
ORIGIN      
        1 cctactacga gcagctgcag tacgccggcg acacccggat ccaggcgctc gtgtcgctct
       61 acgccagcgg cgacgcgcgg ctcatgaaga acgccatcgc gcacctcgac gactcgcgca
      121 ccgcggaggg cgcgacgatg agccgggcgc cgacgcggca gcagcagtac atcccgccgt
      181 tctcgctgtg gtggatcggg atgctgcacg actactacat gtacgtggac gacgaggcgt
      241 tcgtccgcga gcggctcggc ggcgtgcggg ccgtcctgca gttcttcggc cgccaccaga
      301 agccgaacgg ctcgctgggc cgcctgccct ggtggaacta cgtggactgg acgcccgagt
      361 ggccgggcgg cgtacctccg agccagggga acggctccat cgccgtcctc gacctgcagc
      421 tgctgctcgc ctacgactgg gccgcgcacc tcgaggacgc gctcgggtcg aaggccgttg
      481 cgtcggagta ccgggcgtcg gcggagcggc tcaggcaggc catccggccg ctctactggg
      541 acgagggacg gcggttgtac gccgacaccg aggcgcacga ccgctactcg cagcacgcga
      601 acgtcctggc cgtgctcgcc ggcgtgtccg ccggagcgga agcccgcgac ctcgtggccc
      661 gggtcatcga ggacgcgacg atcacgcggt gctcgtacta cttccgccat tacctgcact
      721 cggcggtgaa cctcgtcggc gagggcgacc gctatctctc gctgctggcc gactgggaag
      781 gcatgctcgg ccgcggtctc acgacgttcg ccgagcggta cgacgcgccg ggcgatccgt
      841 cgcgatcgga ctgccacgcg tggagcgcga gcccgaattt cgagatcttc cgcacggtgc
      901 tgggcatcga cacggccgcc gcggggttcg cggcagtgag ggtgcgcccg ttcctcgggg
      961 cgctcggccg tgcttccggc tcgatccccc atccgaaggg cgaggtctcg gtggcgctcg
     1021 tgcgagaggg gcgcgggttg cgggccgaga tcgtactgcc ggaaggcgtc ggcggcgagt
     1081 tctcgtggca gggggtccat cggccccttg cgccgggcgc gaaccggttc acggcgcagc
     1141 ccgccgcgaa gtgatggtgg cgagcgcagc ggcgcaggcg aaagccggcg cctcggcgcc
     1201 ggccggcgcg gacgcggggc cagggacggc gggcgctagg ccagcggggc tccgccgccg
     1261 ggtccggcgc acacgccgcg ctgacacgaa cggccctggc gcggcgggga ggagtggccg
     1321 gcgcgccagg gccgtccggg ggggccgggc cggcctaccg cacgggagtc cgcgcggcgc
     1381 ctggcacggc cgcctcgacc tcttttctgc cgcggacccg gttcgccagc gcccgcagca
     1441 ggtcggtgat cttcatctcg cgcagatcgt cgaagtacga gtagacgacc ggcgtgacga
     1501 gcagggtcag cagcaggcac aggagctggc cgccgataat ggtgaccgcc atcgaggcc
//
LOCUS       DAOSIF010000642         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_69930_length_1551_cov_3.099599, whole genome shotgun sequence.
ACCESSION   DAOSIF010000642 DAOSIF010000000
VERSION     DAOSIF010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69930_length_1551_cov_3.099599"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLT35_14705"
     CDS             complement(<1..1067)
                     /locus_tag="PLT35_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG30524.1"
                     /translation="MTMRTSSSSAVRRAAGLAAVVAAASAAALAAPPQPRRASLVVTG
                     GTVITMDAERRILSPGAVAICGDSIAAVDTPAAIARRFVAADIVRADGAVVLPGLVNT
                     HGHAPMVMYRGLADDLALMDWLQKYIFPAESKTVTPELVRVGTRLAALEMIQSGTTLH
                     TDMYYFEEEVARATKAAGMRGVLAQTVIGFPVPDAKTPADGLARAKRFMEEFRGDPLI
                     VATVAPHSMYTVDAESLKASRDLARTFGVPLVTHLAETDDEVRRSLEAHRLTPAAYLD
                     SLGFWDTPALAAHGVHLTDDDIALLRRRGVALSHNPESNMKLASGAAPIVKCLRAGLA
                     VGLGTDGAASNNDLDMFEAMR"
     gene            1132..>1551
                     /gene="upp"
                     /locus_tag="PLT35_14710"
     CDS             1132..>1551
                     /gene="upp"
                     /locus_tag="PLT35_14710"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOG30525.1"
                     /translation="MAVILVRHPLVQDILLSLRDAQTEPPAFRRMAERISVLLAAEAF
                     RALPVETATVMSPIGPATGQRIASDVIVVPVLRAGLGMLNAVLDLVPNARVGHIGLQR
                     DEMTAIASRYYTKLPPGLARSHVLMIDPMLATGGSAVG"
BASE COUNT          215 a          559 c          540 g          237 t
ORIGIN      
        1 tgacgcatgg cctcgaacat gtcgagatcg ttgttgctgg cggcgccgtc cgtgccgagg
       61 ccgacggcga ggcccgctcg gaggcacttc acaatcggcg cggcgccgct cgccagcttc
      121 atgttgctct cggggttgtg cgacagcgcg acgccccggc gccggaggag cgcgatgtca
      181 tcgtcggtga ggtgaacgcc gtgcgcggcg agtgccggag tgtcccagaa cccgagcgag
      241 tcgaggtagg cggccggcgt gagccggtgt gcctcaagcg accgccggac ctcgtcgtcg
      301 gtttccgcca ggtgggtgac gagcggcacc ccgaacgtcc tggccagatc ccgcgacgcc
      361 ttcagcgact cggcatccac cgtgtacatc gagtgcgggg cgaccgtggc cacgatgagg
      421 gggtcgcccc tgaactcctc catgaagcgc ttcgcgcgcg ccaggccgtc ggccggcgtc
      481 ttcgcgtccg gcaccgggaa gccgatgacg gtctgcgcta ggacgccgcg catgcccgcg
      541 gccttggtgg ccctggccac ctcctcctcg aagtagtaca tgtccgtgtg gagcgtcgtc
      601 ccggactgga tcatctccag ggccgcgagc cgcgtgccca cgcgaacgag ctcgggcgtg
      661 accgtcttgc tctcggccgg gaagatgtac ttctggagcc agtccatgag ggccaggtcg
      721 tcggccaggc cacggtacat caccatcggc gcgtggccgt gggtgttcac gaggcccggc
      781 aagacgacgg cgccatcggc gcggacgatg tccgccgcga cgaaacgcct ggcgatcgcc
      841 gcaggtgtgt ccacggcggc aatcgagtcg ccgcagatcg cgaccgcgcc cggcgagagg
      901 atccggcgtt cggcgtccat cgtgatgacc gtcccgccgg tgacgacgag cgacgcgcgc
      961 ctgggctgtg gcggcgccgc gagcgccgcc gcggaggcgg ccgccaccac ggccgccagt
     1021 ccagccgcgc gccgcacggc tgacgacgag gaggttcgca tggtcatgcc tgcgattcta
     1081 ccgtagggcg ccgcgtagcc gcctcctcgg gcgttcgtgt atagtctccg cgtggccgtc
     1141 atcctcgtcc gtcatccgct ggtccaggac atcctgctgt cgcttcgcga cgctcagacc
     1201 gagcccccgg cattccgccg catggccgag cgcatcagcg tgctgctcgc ggccgaggcc
     1261 ttccgcgccc ttcccgtgga aaccgccacc gtgatgtcgc caatcggccc ggccaccgga
     1321 cagcgcatcg cgtcggacgt catcgtcgtg cccgtcctgc gggccgggct cggcatgctg
     1381 aatgcggtgc tcgacctcgt ccccaacgcc cgcgtcggcc acatcgggct ccagcgagac
     1441 gaaatgaccg cgatcgcctc ccgctactac acgaagctgc cgccggggct cgcccggagc
     1501 cacgtgctga tgatcgaccc catgctcgcc actgggggca gcgcggtcgg g
//
LOCUS       DAOSIF010000643         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_70187_length_1547_cov_4.103217, whole genome shotgun sequence.
ACCESSION   DAOSIF010000643 DAOSIF010000000
VERSION     DAOSIF010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70187_length_1547_cov_4.103217"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLT35_14715"
     CDS             complement(<1..471)
                     /locus_tag="PLT35_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30526.1"
                     /translation="MTDYYASTNGQRIVSCSFSEPWYGAPTCEVILATDAPLVDPVTV
                     TIGNRVARMAVLQQAPFAGSQRALLVGGAARWGKDVDLVPYENPAGIMLSTVLGDVAK
                     ATRSDPANASTGETVVLASDRSLGTLYVPERGPAARLLRELAGEPLVLFPSGSRP"
     gene            complement(468..1274)
                     /locus_tag="PLT35_14720"
     CDS             complement(468..1274)
                     /locus_tag="PLT35_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30527.1"
                     /translation="MSAVADLSPWEYPEEWDPLTLAGVLVPGLVKVTGWKRATNYDVK
                     VGKGTAGATITLAGKPPAKGQIEFRVWTTAQLREWDANVRPLLTLVPHPQATATAPTT
                     AAAPTNQVTSGGSADATTGGGSINNPAKSATPAKGSTEDAYAAPAQPKSLTKADALDI
                     YHPILAELEVTAVLPPEELGQWENSESDGGVGYLRRVVEFLEFKQPGGNITTTPQGST
                     SDGATARGDKYSVGGADANAGTSPTSPSNASGSANDARGATVPPNFGGKP"
     gene            complement(1271..>1547)
                     /locus_tag="PLT35_14725"
     CDS             complement(1271..>1547)
                     /locus_tag="PLT35_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30528.1"
                     /translation="GYASEGAAVGVEKGADLVEGAGAKLGSAMAGGAAGGANAGNARG
                     GKGGLSVTFADGAIRIDGAGKSGLQITEEMLTIVLEKLAASQGLARA"
BASE COUNT          176 a          573 c          549 g          249 t
ORIGIN      
        1 cggccggctg ccgctcggga acagcacgag cggctcgccc gcgagctcgc gcaggagacg
       61 cgcggcgggg cccctctcgg gcacgtacag cgtcccgagg ctgcggtccg atgcgaggac
      121 caccgtctcc cccgtcgacg cgttggcggg gtcgctgcgc gtcgccttgg ccacgtcgcc
      181 gaggacggta gagagcatga taccggcggg gttctcgtag gggacgaggt cgacatcctt
      241 cccccaccgc gccgcgccgc cgacgaggag cgcccgctgc gagcccgcga acggcgcctg
      301 ttgcagcacc gccatgcgcg ccacgcggtt gccgatcgtg acggtgacgg ggtcgacgag
      361 cggcgcgtcc gtcgcgagga tgacctcgca cgtgggcgcg ccgtaccacg gctccgagaa
      421 cgagcacgag acgatgcgct ggccgttggt gctcgcgtag tagtcggtca cggcttgccc
      481 ccgaagttgg gcggcacggt cgcgccgcgc gcgtcgttgg cgctgccgga ggcgttactc
      541 ggagaggtcg gggacgtgcc cgcgttcgcg tcggcgcccc cgacgctgta cttgtcgccg
      601 cgcgccgttg cgccgtcgga cgtcgagccc tggggcgtgg tcgtgatgtt gccgccgggc
      661 tgcttgaatt caagaaactc gaccacgcgc cgcagatagc ccacgccgcc gtcgctctcg
      721 ctgttctccc actgccccaa ctcctcgggc ggaagcacgg cggtcacctc gagctccgcg
      781 aggatcgggt ggtagatgtc gagcgcgtcg gccttcgtga ggctcttggg ctgcgcgggc
      841 gccgcgtacg cgtcctccgt gctgcccttc gcgggcgtcg cgctcttggc tgggttgttg
      901 atgctcccgc cgcccgtggt cgcgtccgca gagccgcccg acgtgacctg attcgtcggc
      961 gccgctgccg tcgtcggcgc ggtcgcggta gcctgcggat gcggcacgag cgtgaggagc
     1021 gggcgcacgt tcgcatccca ctcgcggagc tgcgccgtgg tccacacgcg aaactcgatc
     1081 tgccccttcg cgggcggctt gccggcgagc gtgatcgttg cgcccgcggt gcccttgccg
     1141 accttcacgt cgtagttcgt tgcgcgcttc cagcccgtga ccttgacgag ccccggcacg
     1201 agaacgcccg cgagcgtgag cgggtcccac tcctcggggt actcccacgg cgaaaggtcg
     1261 gcgacggcgc tcatgctcgc gcgagcccct gggaagcggc gagtttttcg agcacgatcg
     1321 tcagcatctc ctcggtgatt tgaagcccgc tcttgcccgc gccgtcgatg cggatcgcgc
     1381 cgtccgcgaa cgtcacgctg aggccgccct tgccgccgcg tgcgttgccc gcgttcgctc
     1441 cgcccgccgc gccgccggcc atcgcgctgc cgagcttcgc gcccgcgccc tcgacgaggt
     1501 ccgcgccctt ctcgacgccc acggccgcgc cctcgctcgc gtagccg
//
LOCUS       DAOSIF010000644         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_70394_length_1544_cov_2.774345, whole genome shotgun sequence.
ACCESSION   DAOSIF010000644 DAOSIF010000000
VERSION     DAOSIF010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70394_length_1544_cov_2.774345"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="fmt"
                     /locus_tag="PLT35_14730"
     CDS             <1..1041
                     /gene="fmt"
                     /locus_tag="PLT35_14730"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOG30529.1"
                     /translation="PDRPPHPEVEPVGEVVVASRSLRAVFFGTPEFAVPSLEALLASR
                     HAVVGAVTQPDRPRGRGQRVSDSPVKRVAAAAGVPVLQPERLKDPAFLEHLRGLQADV
                     GVVAAYGKILPAAVLDLPPLGLVNVHASLLPRYRGAAPVHRAVMAGDAETGVSIMRVV
                     EELDAGPVFSAAAIPIGPDETSEELERSLAGLGARLLLGVIEALASTGAVATPQDESA
                     ATCAARLRKEEGVIDWTAGAGRIHDQVRGLHPWPLAWTWLRGKRVIVIRTRRVQDAPS
                     STLSPGHVVSMAGDALRVQCGSGQLDILLVRPEGRRAMAARDFASGYAVTPGSRFDTP
                     SPPGSSGLDGGC"
     gene            1047..>1544
                     /locus_tag="PLT35_14735"
     CDS             1047..>1544
                     /locus_tag="PLT35_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG30530.1"
                     /translation="MPAPARRAALRALARVREGRSDLAAAIDAERKPLRDPRDRALAS
                     EIALGTTRWRAALDHAIAHAGGRSVDRFDAAVLDVLRSGAFQLLHLDRIPASAAVDDA
                     VDLCREAGHPRAAAAVNAILRRLSRERSRLPMPGPSDPLRHLSVTWSHPRWLAARWMA
                     RYGFGT"
BASE COUNT          176 a          573 c          566 g          229 t
ORIGIN      
        1 cctgatcgtc cgccgcatcc ggaagttgag ccggtcgggg aagtggtagt ggcgtcccgc
       61 tcccttcgcg ccgtgttctt cggcacaccg gaattcgccg tgccgtcgct cgaggcgctg
      121 ctcgcctcgc gccacgccgt ggttggcgcc gtcacgcagc cggatcgccc tcgaggccgg
      181 ggccagcgcg tcagcgacag tccagtcaag cgtgtggctg ctgcggccgg cgtgccggtg
      241 ctccagcccg agcggctgaa ggacccggcg ttcctggagc acctgcgcgg gctgcaggcc
      301 gacgtgggcg tggtggcggc gtacggaaag atcctgccgg ccgcagtgct cgacctgccg
      361 ccgctcgggc tggtcaacgt ccacgcatcc ctgctgcccc ggtaccgcgg cgccgcgccg
      421 gtccatcggg ccgtcatggc gggcgacgcg gagaccggcg tcagcatcat gcgcgtcgtg
      481 gaggagctgg acgcgggccc ggtcttttcg gccgcggcca tccccatcgg gccggacgag
      541 acgagcgagg agctggagcg aagcctggct gggcttgggg ctaggctcct gctcggcgtg
      601 atcgaggccc tggcgtcaac cggcgccgtc gcgacgcctc aggacgaatc ggccgccacc
      661 tgcgcagcgc gcctgcgcaa ggaagagggc gtcatcgatt ggacggccgg cgccggccgg
      721 atacacgacc aggtccgggg ccttcacccc tggccgctgg cgtggacctg gctgaggggc
      781 aagcgcgtga tcgtcatccg aacgcggcgg gtccaggatg cgccctcctc cacgctgtct
      841 cccgggcacg tcgtctcgat ggcaggcgac gcgttgcgcg tgcagtgcgg gtcgggccag
      901 cttgacatcc tgctggtgcg gccggaaggg cgccgggcca tggccgcgcg cgactttgcg
      961 tccggctacg ccgtgacgcc gggctcgcgc ttcgacacgc cgtccccgcc cggctcgtcc
     1021 ggcctggacg gaggctgctg acgcccgtgc cggctcctgc gcgccgcgcg gcgcttcgcg
     1081 ccctcgcgcg tgtgcgcgag gggcgctctg atcttgccgc ggccatcgac gccgagcgga
     1141 agcccctgag agacccccgt gatcgcgcgc tggcgtccga gatcgcgctc ggcacgaccc
     1201 ggtggagggc cgcgctcgac cacgcgatcg cgcacgccgg cggccgcagc gtcgatcggt
     1261 tcgatgccgc tgtcctcgac gtccttcgct cgggcgcgtt ccagctcctc cacctcgacc
     1321 gcatccctgc ctcggccgcc gtcgacgacg cggtcgatct gtgccgggag gccgggcacc
     1381 ctcgcgcggc agccgccgtc aacgcgatcc tgaggcgtct cagccgtgag cgctcgcgac
     1441 tcccgatgcc cgggccctcg gacccgctcc gccacctgag cgtgacctgg tcccatcccc
     1501 gatggctggc ggcgcgatgg atggcgcgct acgggttcgg gacg
//
LOCUS       DAOSIF010000645         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_70441_length_1543_cov_3.805780, whole genome shotgun sequence.
ACCESSION   DAOSIF010000645 DAOSIF010000000
VERSION     DAOSIF010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70441_length_1543_cov_3.805780"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1179)
                     /locus_tag="PLT35_14740"
     CDS             complement(127..1179)
                     /locus_tag="PLT35_14740"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOG30531.1"
                     /translation="MRRVLIPALVLWPALAPAQVAVLSVSPLHLAFDAARPAVSDGQL
                     VRIRNLGSGVLRWRAIPRDPWVRVSPAEGTGPALLEVRIDADALGAGRHESRVTIDAG
                     DADDSPVSVTVTAEVAPRAAAASGPPAAAEAGASRAAAPPGAEPSSPAAAVAAGAAPA
                     APLRFTAPSLPTAVRNLPYSHAVPVAGGTPPYSIRLVQGRLPRGLALKDGSISGAARL
                     QGAYPVVLAVTDSAQPPATVAAALSLRVIVLFADTRLTVSPPALTLSAPRGASAARAR
                     LGISSGRQSLGWTASADAPWLRLFPASGTAPGAVDLEITPRSLARGAHTATVTVVMDG
                     VPGSPARIPVTVIVQG"
     gene            1241..>1543
                     /locus_tag="PLT35_14745"
     CDS             1241..>1543
                     /locus_tag="PLT35_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30532.1"
                     /translation="MKRHVDLLALFHLVWGALSLAAGAATLALALGALAIVASAERTA
                     LGPNVGATFTAGVFFAFAIAAVAWGGLHLWVGRALGRRLAWARVLGLGIAVLNLFFL"
BASE COUNT          200 a          539 c          601 g          203 t
ORIGIN      
        1 gatccattcc ttgatctcgc gccggacggt ctgcgaatgg cccgggatgt agtccacctg
       61 ctcccggatc ttgaggcgca tcatttctcc gcagagcggg cattccttcc tgagttccat
      121 gacggcctac ccctgaacga tgacggtgac ggggatgcgt gccgggctgc cggggacgcc
      181 gtccatgacc accgtgaccg tggcggtgtg ggccccgcgc gccagcgacc tcggcgtgat
      241 ctcgagatcg acggcgccgg gcgccgttcc gctcgccggg aagaggcgga gccagggcgc
      301 gtcggccgac gcggtccagc cgagcgactg gcggccgctc gagatgccga gccgcgcgcg
      361 ggccgcgctc gcaccccgcg gcgcagacag cgtcagggcc ggcggactga ccgtgagcct
      421 ggtgtcggcg aagagcacaa tcacccggag ggagagcgcg gccgccacgg tggccggcgg
      481 ctgggccgag tcggtgaccg cgaggaccac gggatacgcg ccctgaagac gtgccgcgcc
      541 ggaaatcgag ccgtccttca gcgccaggcc gcgcggcagg cgcccctgca ccaggcgaat
      601 cgagtagggg ggcgtcccgc ccgcgacagg gacggcgtgc gagtagggca ggttccggac
      661 cgcggttggc agcgagggcg cggtgaagcg aagcggcgcg gctggggcgg ctccggctgc
      721 gacggccgcc gcgggagagg agggttcagc gcccggcggc gccgcggccc ggctggcgcc
      781 ggcttccgcg gccgccggcg gccccgatgc cgccgctgcc cgcggcgcca cctccgctgt
      841 gacggtgacg gagaccggcg agtcgtcggc atcgcccgcg tcgatcgtca cgcggctctc
      901 gtggcggccg gcgcccaggg cgtcggcgtc gatgcgcacc tcgaggagcg cagggcccgt
      961 gccctcggcc ggagagacgc gcacccatgg atctcgcggg atcgccctcc atcgcaggac
     1021 gccgcttccc aggttgcgaa tccggacaag ctggccgtcc gacaccgcgg gacgcgcggc
     1081 gtcgaaggcc agatgcagcg gcgacaccga caagacggcg acctgcgccg gggcgagcgc
     1141 gggccagagc acgagcgcgg gaatcagcac gcggcgcacg ggtcgtggca gaatactagc
     1201 acgcgcatcg gtcggaggcc cgcgccggtg cgcggcatgg atgaagcggc acgtcgatct
     1261 gctcgcgttg ttccacctcg tctggggtgc gctgtcgctg gcggctggcg cggcgacact
     1321 cgcgctggcg ctcggcgcgc tggccatcgt ggcgtcggcc gaacgaacgg ccctcgggcc
     1381 gaacgttgga gcgaccttca cggcgggcgt gttcttcgcg ttcgccatcg cggccgtcgc
     1441 gtggggaggc ctgcacctct gggtcggccg cgctttgggc aggcgcctcg cctgggcccg
     1501 ggtcctcggg cttgggatcg ccgtgctgaa cctgttcttt ctg
//
LOCUS       DAOSIF010000646         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_70658_length_1540_cov_3.519192, whole genome shotgun sequence.
ACCESSION   DAOSIF010000646 DAOSIF010000000
VERSION     DAOSIF010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70658_length_1540_cov_3.519192"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1540)
                     /locus_tag="PLT35_14750"
     CDS             complement(<1..>1540)
                     /locus_tag="PLT35_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30533.1"
                     /translation="EAFRRSLDVAGHVSFVPGGTELSSGRLGAIVPVAVGQALGLRAK
                     RGPEAAVICHCGDAGWISGQSLNGYVAASQHRAPLLFVMHRNGIQLSGTTARVMDRDP
                     RPVIASLGIQIVEIASLHDRPALFGAYRDGLALAREGQPALIYPTGYRSGNGRAVTVA
                     AFGEQHGIGAEVARFAAEHGVPLDTEIWIPGSLMSFRDARAMCQCLFYVNGLPGGEGH
                     HDGGMKGRDEAATLANPLLRLTPAEAEALSRLRAKPRRKVVTTARPPKGSPNLVLEAA
                     DLDPVSLPAPGTSVSPRAGSEAAYDAIARRHPGRCFFVSCDLDPSTKLGKAASRVPRD
                     HRFEMSIQEQAAALMANGLAFSSREPQLNVFATFAAFFEGIAREGFEFWRYQRNLTGA
                     NEGLNVVMHLSHVGACTGRDHFSGWSLDWINLGLGYLPYLRRFYAPADARAAFVAVRD
                     AAAAMGGHIVAVPRDNLPVLAKADGSPLWNAGDAWSPATVCRQRAGARTVVLAVGAPA
                     YLAVA"
BASE COUNT          218 a          548 c          577 g          197 t
ORIGIN      
        1 ggcgacagcc aggtaggcgg gcgcgcccac ggccaggacg acggtccggg cgcccgccct
       61 ctggcggcac acggtggccg gcgaccacgc gtcgccggca ttccacagcg gcgagccatc
      121 ggccttcgcg agcaccggca ggttgtctct cggaacggcg acgatgtggc cgcccatggc
      181 ggcggcggcg tctctgactg ccacgaacgc cgcgcgggcg tcggccggcg cgtagaagcg
      241 ccgcaggtac ggcaggtagc cgagtcccag gttgatccag tcgaggctcc agccggagaa
      301 gtggtcgcgg cccgtgcagg ccccgacgtg cgagaggtgc atgacgacgt tgaggccctc
      361 gttcgcgccg gtcaggttgc gctggtagcg ccagaactcg aagccctcgc gcgcgatgcc
      421 ttcgaagaac gcggcgaagg tcgcaaagac gttgagctgc ggctcccggc tgctgaaggc
      481 gaggccgttc gccatcaggg cggcggcctg ctcctggatg ctcatctcga accggtgatc
      541 gcgcggcacg cgcgacgcgg ccttgcccag cttcgtcgag gggtcgagat cgcacgacac
      601 gaagaagcag cgtccggggt gcctccgcgc gatcgcgtcg tacgccgcct cggagcccgc
      661 gcgcggcgac acggatgtcc cgggcgccgg caggctgacg gggtcgaggt cggccgcctc
      721 aagcacgagg ttcggcgagc ccttcggcgg gcgcgcggtc gtgacgacct tccggcgcgg
      781 cttcgcgcgc aggcgcgaca gcgcctcggc ctcggccggc gtgagccgca gaagcgggtt
      841 cgccagcgtg gcggcctcgt cgcgcccctt catgcctccg tcgtggtggc cctcgccgcc
      901 cggcaggccg ttgacgtaga acaggcactg gcacatggct cgcgcgtccc ggaagctcat
      961 cagcgacccg ggaatccaga tctcggtgtc gagcggcacg ccgtgctcgg cggcaaaccg
     1021 cgcgacctcc gcgccgatgc cgtgctgctc gccgaacgcc gcgacggtga cggcgcggcc
     1081 gttgccggac cggtagcccg tcggatagat cagcgccggc tggccttccc gggccagcgc
     1141 cagcccgtcg cggtaggcgc cgaagagcgc cgggcggtcg tgcagcgacg cgatctcgac
     1201 gatctggatg ccgagcgacg cgatgaccgg ccgcggatcg cggtccatca cgcgcgcggt
     1261 cgtgccggag agctggatgc cgttgcggtg catcacgaag agcagggggg cgcggtgctg
     1321 gctcgcggcc acgtaaccgt tcagcgactg ccccgagatc cagccggcgt cgccgcagtg
     1381 gcagatgacg gccgcctcgg gccctctctt cgccctgagg ccgagcgcct gaccgacggc
     1441 gacgggaacg atcgcgccga ggcgcccgga actcagctcg gtgccgccgg gcacgaacga
     1501 cacgtgcccg gcgacgtcga ggctgcgccg gaacgcctca
//
LOCUS       DAOSIF010000647         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_70662_length_1540_cov_3.276768, whole genome shotgun sequence.
ACCESSION   DAOSIF010000647 DAOSIF010000000
VERSION     DAOSIF010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70662_length_1540_cov_3.276768"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="PLT35_14755"
     CDS             complement(<1..1305)
                     /locus_tag="PLT35_14755"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG30534.1"
                     /translation="MTEPGRNEAETIRLEKPGWRTRARMWLAGTTIQSRLARTFTLAS
                     LAPALVTAVVGVGMIRHGVYAQAQAQVNANLESAKDIYQSALDRLQDAIRIHATRMVI
                     YGALSRGDPSGLAEEMDRIRRAERLDALTIVDADGRVFYRSLNPSGPRGEVTEDRVIR
                     QALERLQPVAATMIVPPDVLLRNSPALAARASIKIEPTPRARPGSQTEITAGLMLRAS
                     APVTTPAGRMLGVLDGAILLNRNPDIVDAIRRTVFKEEEYEGRPTGTATIFENDVRVS
                     TNVRGADGTRAIGTRASAEVADAVLSRGETWRGRAFVVQDWYLAAYAPIRDLGGATIG
                     MLYVGVLEKPFMDSLWRSLLVFLGIAAGGVALVNLIAVRVAKRISAPIHELTVAARRI
                     AEGEYARVAERNTDDEIGDLSRSFNTMTTELQAAHQALRESAD"
     gene            complement(1319..>1540)
                     /locus_tag="PLT35_14760"
     CDS             complement(1319..>1540)
                     /locus_tag="PLT35_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30535.1"
                     /translation="AGPSPHAMGLPSVVRLGCRSGVPLPARIHVVAVEAEDTASCAEG
                     LTPAVARAVPEAVEEVWSVLARLGTGAPA"
BASE COUNT          199 a          567 c          556 g          218 t
ORIGIN      
        1 gtccgcgctc tcccgcagcg cctggtgcgc ggcctggagc tcggtcgtca tcgtgttgaa
       61 gctgcgggag aggtccccga tctcgtcgtc cgtgttgcgc tcggcgacgc gcgcgtactc
      121 gccttcggcg atccgccggg ccgccacggt cagctcgtga atcggggcgg agatgcgctt
      181 cgccacgcgc acggcgatga ggttgaccag cgcgacgcct ccggcggcga tgccgaggaa
      241 caccagcagg ctgcgccaga ggctgtccat gaacggcttc tcgaggacgc cgacgtagag
      301 catgccgatc gtggcgccgc ccagatcgcg gatgggcgcg taggcggcca ggtaccagtc
      361 ctggacgacg aacgcgcgcc cgcgccaggt ctctccgcgg gacagcacgg cgtcggcgac
      421 ttcggcggac gcccgggtcc cgatggcgcg cgtgccgtcc gccccccgca cgttcgtcga
      481 gacgcgcacg tcgttctcga agatcgtggc ggtgccggtc ggacggccct cgtactcctc
      541 ctccttgaac accgtccggc ggatcgcgtc gacaatgtcg gggttgcggt tgagcaggat
      601 ggcgccgtcg agcacgccga gcatgcggcc cgccggcgtc gtgaccggcg ccgaggcccg
      661 aagcatcagg ccggcggtga tctccgtctg ggacccgggc cgggcgcgcg gcgtcggctc
      721 gatcttgatc gaggcccgcg ccgccagcgc cggcgaattc cgcaggagca cgtcgggcgg
      781 cacgatcatc gtcgcggcca ccggctgcag ccgctcgagg gcctggcgga tgacgcggtc
      841 ctcggtcacc tcgcccctcg gcccggacgg gttgagcgag cggtagaaca cgcggccgtc
      901 ggcgtccacg atcgtcaggg cgtcgagccg ctcggcccgc ctgatgcgat ccatctcctc
      961 ggcaaggccg gagggatcgc ccctggacag cgccccgtag atgaccatcc gcgtcgcgtg
     1021 gatccggatg gcgtcctgca gccggtcgag ggcggactgg tagatgtcct tcgccgactc
     1081 gaggttggca ttcacctgcg cctgcgcctg ggcgtagacg ccgtggcgga tcatgccgac
     1141 gcccacgacc gccgtcacca gcgccggggc caggctcgcg agggtgaagg tccgggcgag
     1201 gcggctctgg atcgtggtgc cggcgagcca catcctggcc cgcgtgcgcc agccgggctt
     1261 ctcgagccgg atcgtctcgg cctcattccg gccgggttcc gtcatggctc cgccgtcctc
     1321 acgccggcgc gcccgttccc aggcgcgcca gcaccgacca gacctcctcg accgcttccg
     1381 gcaccgcgcg ggcgaccgcc ggagtcaacc cttccgcgca cgacgcggtg tcctcggcct
     1441 cgaccgccac gacgtggatc cgcgccggca gcggaacgcc gctccggcag cccagcctga
     1501 cgaccgacgg aaggcccatc gcgtggggcg aggggccggc
//
LOCUS       DAOSIF010000648         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_72116_length_1519_cov_3.938525, whole genome shotgun sequence.
ACCESSION   DAOSIF010000648 DAOSIF010000000
VERSION     DAOSIF010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72116_length_1519_cov_3.938525"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLT35_14765"
     CDS             complement(<1..956)
                     /locus_tag="PLT35_14765"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOG30536.1"
                     /translation="MRLYTSGTTDAGCVRTNNEDYIYGGRLTTGDYLFVVADGMGGHH
                     AGEVASRRAVTRLVDLVEGGVPEPIRDSLGAMFAQINSELFHDNQKAEPEQRMGTTLT
                     ALLARGNLAYVAHVGDSRLYRYRNGSAADGQPAPLTRITDDDSLVGALVRDGAISEAE
                     ARVHPRRSVLNQAIGMREHINPQIRGPLEIQPGDKYVLCSDGLSSVVSDSAIQEFLDL
                     PSSEHITKQLVSRARSAGGPDNISVIVVSTEPDAAPSSEGDTSPEIGPAARPGRQAQP
                     SRRALRWFLLGLLALLLAAVVYLLVGGTAGERERSAGGTAQGP"
     gene            complement(992..>1519)
                     /locus_tag="PLT35_14770"
     CDS             complement(992..>1519)
                     /locus_tag="PLT35_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30537.1"
                     /translation="RDLADILARMQRLITQKEYARAISLGRSVSGPGAYSRELLQMVA
                     LAYFCDGRQTQSSAYVSRALDQTGSIQYPMLFETRQKRYVRGTLEITRRGLSFRTGLT
                     TAPLLNIPLAGIKSVSDDVRGDIADLFKKKQNRKNPILIIKSRQDAQYRLQLDRDDGD
                     LKKFIKHVITTLRGS"
BASE COUNT          226 a          508 c          505 g          280 t
ORIGIN      
        1 gggccctgcg cggtcccccc ggcagatcgc tcccgctcgc cggcggtgcc gccgacgagc
       61 aggtacacga ccgcggccag gagcagggcc agaaggccca ggaggaacca cctgagcgct
      121 cgccggctcg gctgggcctg ccggccgggc cgggcggcag gcccgatctc cggcgaggtg
      181 tcgccctcgg acgacggcgc ggcgtccggt tcagtgctca cgacaatgac gctgatgttg
      241 tcaggccctc cggcggacct ggctcggctt acgagctgct tcgtgatgtg ttcgctcgag
      301 gggagatcca ggaattcctg gatggccgaa tcgctcacga ccgacgacaa cccgtcggaa
      361 cacaacacgt acttgtcgcc cggctgaatc tccaacgggc cgcggatctg gggattgatg
      421 tgctcgcgca tgccgatggc ctggttgagc acgctgcgcc tgggatggac ccgggcctcc
      481 gcttcgctga tggcgccgtc gcggaccagc gcgccaacca gcgagtcgtc gtcggtgatg
      541 cgggtcaagg gcgcgggctg gccgtcggcg gcgctgccat tccggtaacg gtagagacgg
      601 ctgtccccga cgtgggccac atacgccagg ttgcctctcg ccagcaatgc ggtcagggtc
      661 gtgcccatgc gctgctcggg ctcggccttc tggttgtcgt ggaagagctc gctgttgatc
      721 tgcgcgaaca tggcgcccag cgagtcccgg atcggttccg gcaccccgcc ctcgaccagg
      781 tccaccagcc gcgtcaccgc ccgccggctg gccacttcgc cggcatgatg ccctcccatc
      841 ccatcggcaa ccacgaacag gtagtccccg gtcgtcaggc ggccgccgta gatgtagtcc
      901 tcgttgttgg tccggacgca gccggcgtcc gtggtcccgc tggtgtagag cctcatggcc
      961 gatgacagat cgcctgacaa cccatctgtg ctcaggatcc cctgagcgtg gtgatgacgt
     1021 gcttgatgaa cttcttcagg tcgccgtcgt cccgatccag ctggagacga tactgggcat
     1081 cctgtcggct cttgatgatc agaatcgggt tcttgcgatt ctgcttcttc ttgaacaggt
     1141 ccgcgatgtc gccgcgcacg tcgtcggaga cgctcttgat gcctgccagc gggatgttga
     1201 gcagcggggc ggtcgtcagg ccggtgcgaa aggagagccc gcgccgcgta atctcgagcg
     1261 tgcccctgac gtaacgtttc tggcgggttt caaacagcat cgggtattgg atcgagccgg
     1321 tctgatccag ggcccggctg acgtaggcgc tgctctgcgt ttgccgcccg tcgcagaagt
     1381 acgccagcgc aaccatctgc agcagctccc tggagtatgc gccgggcccc gagacgctcc
     1441 ttcccagact gatggcccgt gcgtattcct tctgggtgat cagccgctgc atcctcgcca
     1501 ggatgtcggc cagatcccg
//
LOCUS       DAOSIF010000649         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_72547_length_1513_cov_3.075446, whole genome shotgun sequence.
ACCESSION   DAOSIF010000649 DAOSIF010000000
VERSION     DAOSIF010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72547_length_1513_cov_3.075446"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1513
                     /locus_tag="PLT35_14775"
     CDS             <1..>1513
                     /locus_tag="PLT35_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG30538.1"
                     /translation="SEPATFAIRLRQHLRQHLDELLPDGEGVARARWEGSPFLGLLAF
                     QFKDAEIFHGREREVDEVLLALHRRDREGCAFLLILGASGSGKSSLVRAGVIPQLVLH
                     PLSSEAGPWRWAALTPGVHAGDLIGGLTRALAAETALPALRGDAAALASLAGALVRDP
                     RGAWELRIREALDQLNVGGKPARLVLAIDQLEECFTHPAITAPECESLFAALDVLARS
                     GRVWVLATLRSDAYQACQRYPSLMALKGESGQFDLLAPTPADLRRIITEPARLAGLRF
                     ERDPRTHHALDERILTDAAGHADALPLLEFALDRLYERRTAEGELTFEAYERIGRVEG
                     ALREHAEELYRSLPERARRTFDTVMSALVTVQGSDQAVTRRRARMDELALNDDQRALV
                     EQFTQGRILVADRDETGRATLTVAHEALLAAWPRLADWIARNQDLLRVRARVDSEQAA
                     WEEHGRDERYLLAAGIRLSEGEDLLAQWGDRLSPGAVQFISQSASAARAAELRG"
BASE COUNT          198 a          513 c          596 g          206 t
ORIGIN      
        1 cagcgagccg gcgacattcg ccatccggct gcgccagcac ctgcgccagc acctggatga
       61 gctgctgccc gacggcgagg gcgtcgcacg cgcccgctgg gagggctcgc cgttcctggg
      121 cctgctcgcg ttccagttta aggatgccga gatcttccac gggcgcgagc gcgaagtgga
      181 cgaggtgctg ttggcgctgc accggcgcga ccgggaaggc tgcgcgtttc tgttgatcct
      241 cggggcgagc ggcagcggca agtcctcgct cgtgcgcgcg ggcgtcatcc cgcagctggt
      301 cctccatccg ctgagttcgg aggccgggcc gtggcgctgg gcggcgctga cgccgggggt
      361 gcatgcgggg gacttgatcg gcgggctgac gcgggcgctg gccgccgaga cggccctgcc
      421 ggcgctgcgc ggcgatgccg ctgcgctcgc cagcctggcc ggcgcgctcg ttcgcgaccc
      481 acggggggcc tgggagcttc ggatccggga ggcgctcgat caactgaacg tcggcggcaa
      541 gccggcccgg ctcgtactgg ccatcgacca gctcgaggag tgcttcactc atcccgccat
      601 taccgcgccg gagtgcgaga gcctgttcgc cgcgctggac gtgctggcgc gcagcggccg
      661 cgtctgggtg ctggcgaccc tgcgcagcga cgcctaccag gcgtgccagc gctatccctc
      721 gctgatggcg cttaaaggcg agtcgggcca gttcgacctg ctggcgccga cgccggccga
      781 cctgcggcgc atcatcacag aaccggcgcg cctggccggc ctccgcttcg aacgcgaccc
      841 gcgcacgcat catgcgctgg acgagcggat cctgaccgat gcggcgggcc acgccgacgc
      901 gctgccgctg ctcgagttcg cgctggaccg gctatacgag cggcgcacgg ccgagggcga
      961 gctgaccttt gaggcctatg agcggatcgg ccgggtcgag ggggcgctgc gcgagcatgc
     1021 cgaggagctc taccggtcgc tgcccgaacg cgcgcggcgg acgttcgaca cggtcatgag
     1081 cgcgctggtg acggtgcagg ggagcgacca ggcggtgacg cggcggcggg cgcgcatgga
     1141 tgagctcgcg ctgaacgacg atcagcgggc gctggtcgag caattcacgc aggggcgaat
     1201 cctggtcgcc gaccgcgacg aaacggggcg ggcgacattg accgtcgcgc acgaggcgct
     1261 gctggcggcc tggccgcggc tcgccgactg gatcgcgcgg aaccaggacc tgctgcgcgt
     1321 gcgcgcgcgg gtggacagcg agcaggcggc ctgggaggag cacggccgcg acgagcgcta
     1381 tctgctggcg gccggcatcc gcctgagcga gggggaggat ctgctggcgc agtggggcga
     1441 ccggctctcg ccgggggcgg tgcagttcat cagccagtcg gccagcgccg cgcgcgcggc
     1501 ggagctgcgc ggc
//
LOCUS       DAOSIF010000650         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_72966_length_1507_cov_4.346419, whole genome shotgun sequence.
ACCESSION   DAOSIF010000650 DAOSIF010000000
VERSION     DAOSIF010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72966_length_1507_cov_4.346419"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLT35_14780"
     CDS             complement(<1..290)
                     /locus_tag="PLT35_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30539.1"
                     /translation="MNDSRLCGNTDVLMSYLYGDGDEQERLDFGAHLERCERCAAEIA
                     GLQAVRRDMAAWSPPETVLGFRVVRAPDPARSRWAWLRPAAMPAWAQLAAASL"
     gene            complement(287..883)
                     /locus_tag="PLT35_14785"
     CDS             complement(287..883)
                     /locus_tag="PLT35_14785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG30540.1"
                     /translation="MSDTDEELVARSIAGDTDSFNQLILRWERPIYALAYRQTGREED
                     ARDIVQDAFLRAFRALPGFKGEAKFSSWLYRITLNLCRDFLRRTRRAPFLPAPEGMDV
                     TELAPAGAAREETVEDVVSRKDLSRHVARAMGRLPEEQRSAILLKEYHGLTFQEIAEL
                     QGVPLSTVKTRLYQGLTVLRRELDRRGSGPARAEGRRP"
     gene            1102..>1507
                     /locus_tag="PLT35_14790"
     CDS             1102..>1507
                     /locus_tag="PLT35_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG30541.1"
                     /translation="MIQLTAVTKSFGARVLFEDVTWQVSDRECVGLCGPNGAGKTTLL
                     RIFAGLDEADAGAVVKPAGLRVGYLPQDGLTHEGKTLHEEASLAFEWLLEVRRELEDI
                     ETRLADPGVPTGEHELMLHRYADLQDRFRLHDG"
BASE COUNT          219 a          551 c          504 g          233 t
ORIGIN      
        1 agcgacgccg cggcaagctg cgcccacgcc ggcatggcag ccggcctgag ccaggcccac
       61 cggctcctcg ccgggtcggg cgcgcgcacg acacggaatc caagcaccgt ctccggcggc
      121 gaccacgccg ccatgtcgcg ccgcacggcc tgcagcccgg cgatctccgc cgcgcaccgc
      181 tcgcagcgct ccaggtgcgc gccgaagtcg agccgttcct gctcgtcccc gtctccgtac
      241 aggtaggaca tcaggacgtc cgtgttgccg cacagtcgtg agtcgttcat ggccttctgc
      301 cctcggccct cgccgggccg ctgccccgcc gatcgagttc ccgccgcagg acggtcagtc
      361 cctggtatag gcgcgtcttg accgtgctga gcggcacgcc ctgcaactcg gcgatctcct
      421 ggaacgtcag cccgtggtac tccttcagca ggattgccga ccgctgctcc tcgggcagcc
      481 gtcccatcgc ccgcgccaca tgccggctga ggtccttccg cgagacgaca tcttcgaccg
      541 tctcctcccg tgcggcgccc gccggcgcga gctcggtgac gtccatgccc tccggtgccg
      601 gcaggaacgg cgcacgccgc gtccgccgca ggaagtcgcg gcagagattg agcgtgatcc
      661 ggtagagcca ggacgagaac ttcgcttcgc ccttgaagcc gggcagcgcc cgaaacgcgc
      721 gcaggaacgc gtcctgcacg atgtcgcgag cgtcctcctc gcggccggtc tggcggtacg
      781 ccagggcgta gatcggccgc tcccatcgca ggatgagctg gttgaagctg tccgtgtcgc
      841 ccgcgatgga acgcgcgacg agttcctcgt cggtgtcgct catgtcgtcg cgatcgccgc
      901 tgatctcctg gcgctcccgg cgcgccggct catccggccc cgcattcaac aacttagacg
      961 gatgggaaac ggcggcagtc gggatccgat tataaaccgc ctaacccctt ggcgaaacgg
     1021 gacttgccag tatggtacac tagagcgttt gcgcgggccg gagccgagcg tccgtggccg
     1081 cgcccccggg cccgggtttc gatgatccag ctgaccgccg tcaccaagtc gtttggcgca
     1141 cgcgtcctgt tcgaggacgt gacgtggcag gtgtcggacc gcgagtgcgt gggcctgtgc
     1201 gggccgaacg gcgccggcaa gacgacgctg ctccgcattt tcgcgggcct cgacgaagcc
     1261 gacgcaggcg ccgtcgtcaa gcccgccggc ctgcgcgtgg ggtacctgcc gcaggacggc
     1321 ctcacgcacg agggcaagac gctccacgag gaggcctctc tcgccttcga gtggctgctc
     1381 gaggtcaggc gggagctcga ggacatcgag acgcgcctgg ccgatcccgg ggtcccgacc
     1441 ggcgagcacg agctgatgct ccaccgctac gcggacctgc aggatcggtt ccggctgcac
     1501 gacggct
//
LOCUS       DAOSIF010000651         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_73128_length_1505_cov_2.901379, whole genome shotgun sequence.
ACCESSION   DAOSIF010000651 DAOSIF010000000
VERSION     DAOSIF010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73128_length_1505_cov_2.901379"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /gene="guaB"
                     /locus_tag="PLT35_14795"
     CDS             <1..1383
                     /gene="guaB"
                     /locus_tag="PLT35_14795"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG30542.1"
                     /translation="RIRVQVPVVSAAMDTVTESRLAIAMAQQGGLGVIHKNLTIEEQA
                     SEVDRVKRSESGMIVNPITLSPSHRIHEALALMNHYRISGVPITEDGSKEGRLVGILT
                     NRDLRFEVNIDRPIHEVMTRENLITVPVGTTLDEAREILHRHKVEKLLVVDRHFMLKG
                     LITVKDIQKVIKYPLASKDALGRLRVGAAVGVGRDTLDRAAALVAAHVDVLVVDTAHG
                     HAHKVMETVAGLRAHFPDVDLVAGNVATGQATTDLIRLGVDAVKVGIGAGSICTTRVV
                     AGIGVPMVSAVLDCARAAAGTDVPIIADGGIRYSGDITKAMAVGADAVMVGSLFAGTD
                     ESPGEVVLYQGRSFKEYRGMGSIGAMRKGSRDRYFQDDFDLDAPGSGSEKLVPEGIEG
                     RVPYRGSVAGVIHQLVGGLRAGMGYVGCATIAQLQQEARFIRVTPAGVREGHVHDVVI
                     TKEAPNYRSE"
BASE COUNT          255 a          515 c          531 g          204 t
ORIGIN      
        1 cgaatccgcg tgcaggtgcc agtcgtcagc gccgccatgg acacggtcac cgagtcccgc
       61 ctcgccatcg ccatggccca gcagggcggg ctcggcgtga tccacaagaa cctcaccatc
      121 gaggagcagg catccgaggt ggaccgcgtg aagcggtcgg agagcgggat gatcgtcaac
      181 ccgatcaccc tctccccgag ccatcgcatc cacgaggcgc tcgcgctcat gaaccactac
      241 cggatctccg gcgtgccgat cacggaggac ggctcgaagg agggccggct cgtcggcatc
      301 ctcacgaacc gcgacctgcg gttcgaggtc aacatcgacc gcccgatcca cgaggtgatg
      361 acgcgggaga acctgatcac cgtccctgtc ggcaccaccc tggacgaggc gcgggagatc
      421 ctccacaggc acaaggtcga gaagctgctc gtcgtcgatc gccacttcat gctcaagggc
      481 ctgatcaccg tcaaggacat ccagaaggtg atcaagtatc cgcttgccag caaggacgcg
      541 ctcggccgtc tgcgcgtcgg cgccgcggtc ggggtcggca gggacacgct ggaccgggcg
      601 gccgcgctcg tggcggccca cgtggacgtc ctcgtcgtgg acacggcgca cggccacgcg
      661 cacaaggtga tggagacggt tgccggactg cgagcccact tccccgacgt ggatctcgtc
      721 gcgggcaacg tggccaccgg gcaggcgacc acggacctca tccgcctggg cgtcgacgcg
      781 gtcaaagtgg gcattggcgc cggctcgatc tgcacgacgc gcgttgtcgc ggggatcggc
      841 gtgccgatgg tgagcgccgt gctcgactgc gcgcgggccg cggcgggcac ggacgtgccg
      901 atcatcgccg acggcggcat tcggtattcc ggggacatca ccaaggcaat ggccgtcggc
      961 gccgacgcgg tgatggtcgg cagcctgttc gcgggcacgg acgagagccc gggcgaagtg
     1021 gtgctctacc agggccggag tttcaaggaa taccgcggta tgggctcgat cggcgcgatg
     1081 cggaaaggat cccgggaccg gtacttccag gacgacttcg acctcgacgc cccggggagc
     1141 ggatcggaga aactcgtgcc tgagggcatc gagggccggg tgccctaccg cggcagcgtc
     1201 gccggggtca ttcaccagct cgtcggcggg ctccgcgccg gcatggggta cgtcgggtgc
     1261 gccacgatcg cccagctcca gcaggaggcg cgcttcatcc gcgtcacccc ggccggcgtg
     1321 cgcgaggggc acgtgcacga cgtcgtcatc acgaaggaag cgccgaacta ccgctctgaa
     1381 tgacgacggc gccaggcggt cagagcgcga acggcgggcc ggccgccgcc tccacgacca
     1441 gcgcgtccgg tccttcgagc gcgacgcgcc ggctgtcgcc gccgagatcc acgatgagca
     1501 cccgg
//
LOCUS       DAOSIF010000652         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_73201_length_1504_cov_3.661836, whole genome shotgun sequence.
ACCESSION   DAOSIF010000652 DAOSIF010000000
VERSION     DAOSIF010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73201_length_1504_cov_3.661836"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PLT35_14800"
     CDS             <1..926
                     /locus_tag="PLT35_14800"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HOG30543.1"
                     /translation="NKLFSWGADEDPDVPVTDYADGVEQTDFVSEQPDRLYGMTQEMR
                     EGYAVGRQAEAESTFTKHNDPVVQKKKALKRLYRQLEVASCSAQEQRDATSSLGSRMR
                     GAFAALAAYGSGTQVFLPFAESMACTAAQLYTGATGDFDDDALEDMLVEIASRQKEST
                     ELVLLCGIQLKRAMSNWTKRDASASGAYNVSIRKPNEQFIVDNVVDVFKMDGGTVRSM
                     VSYNLMLNTATGAATAQTPWAGLLIQPNYWKFRELFGLELKEIEDKGQGKSGFYRIMK
                     GLQCSVPRRNGYILPTVDDSSGASSGASSAA"
     gene            1064..>1504
                     /locus_tag="PLT35_14805"
     CDS             1064..>1504
                     /locus_tag="PLT35_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30544.1"
                     /translation="MINFVSVSALNVFEGVLRAQGLDPATANLSTAEKARVATLVSRA
                     LAKAWRMTMWPQLLVVKRIEFRQTWDADDSYETDDEVYYAEHYWRSLVDGNSGTTPGT
                     DAAKWAAAEGDMVYSVNYAEHGIDEMDLEAGCFSADPERVRDPKK"
BASE COUNT          279 a          442 c          586 g          197 t
ORIGIN      
        1 ccaacaagct cttctcgtgg ggcgccgacg aggatccgga cgtgccggtg acggactacg
       61 ccgacggcgt ggagcaaacc gacttcgtct ccgaacagcc cgaccggctg tacggcatga
      121 cgcaggagat gcgcgaaggc tacgccgtgg ggcgccaggc cgaggccgag agcacgttca
      181 cgaagcacaa cgacccggtg gtgcagaaga agaaggcgct caagcggctg taccgccagc
      241 tcgaagtggc ttcctgctcc gcgcaggagc agcgcgacgc gacttcctcg ctgggaagcc
      301 ggatgcgcgg cgcgttcgcg gccctggccg catacggaag cggcacgcag gtgttcctgc
      361 cgttcgccga gagcatggcg tgcacggcgg cgcagctcta cacgggcgcg accggggact
      421 tcgacgacga cgcgctggag gacatgctcg tggagatcgc gtcgcgccag aaggagtcca
      481 cggagctcgt gctgctctgc ggcatccagc tcaagcgcgc gatgtccaac tggacgaagc
      541 gcgacgcgtc ggcgagcggc gcctacaacg tgagcatccg caagccgaac gaacagttca
      601 tcgtggacaa cgtggtggac gtgttcaaga tggacggcgg cacggtgcgc tccatggtga
      661 gctacaatct gatgctcaac acggccacgg gcgcggctac ggcgcagacg ccgtgggccg
      721 ggctgctgat ccagccgaac tactggaagt tccgggagtt gttcggcctg gagctgaagg
      781 agatcgagga caagggccag ggcaagagcg gtttctaccg catcatgaag gggctccagt
      841 gctcggtgcc gcgcaggaac ggctacatcc tgccgacggt ggatgacagc tccggcgcca
      901 gctccggcgc ttcgagcgcg gcgtagcggg gaccaggccc ccggcgcggt cgaggcccgc
      961 gccgggggat ttgcgccgcc gccggcggcg cccggcgcac gcgccgggac acaggacgcc
     1021 cggcgcacgc gccgggacac aggacggaac ggggggaggc ggcatgatca acttcgtgag
     1081 cgtgagcgcg ctgaacgtgt tcgagggggt gctgcgggcg caggggctgg acccggcgac
     1141 ggccaacttg agcacggctg agaaggcgcg ggtggcgacg ctggtgagcc gggccctggc
     1201 gaaggcgtgg cggatgacga tgtggccgca gttgctggtg gtgaagcgga tcgagttccg
     1261 gcagacgtgg gacgcggacg attcgtacga gacggacgac gaggtgtatt acgccgagca
     1321 ctactggcgg agcctggtgg acggcaactc ggggacgacg ccggggacgg acgcggcgaa
     1381 gtgggcggcg gcggaaggcg acatggtgta ctcggtgaac tatgccgagc acgggataga
     1441 cgagatggat ctggaggctg ggtgcttcag cgcggatccg gagcgggtga gagacccgaa
     1501 gaag
//
LOCUS       DAOSIF010000653         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_73208_length_1504_cov_3.269151, whole genome shotgun sequence.
ACCESSION   DAOSIF010000653 DAOSIF010000000
VERSION     DAOSIF010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73208_length_1504_cov_3.269151"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1504)
                     /locus_tag="PLT35_14810"
     CDS             complement(<1..>1504)
                     /locus_tag="PLT35_14810"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG30545.1"
                     /translation="ALEKYAKDFTALAEAGKLDPVIGRDEEVRRVIQVLSRRTKNNPV
                     LIGEPGVGKTAIVEGLAQRIVAGQVPRDLADKRILALDISLVVAGTKYRGQFEERLKA
                     IMKELAEHTNVIVFIDELHTLVGAGSAEGSLDAANILKPALSRGEIRCIGSTTPAEYR
                     KYIERDRSLERRFQAIKVEAPDEAHTIDILLGIKDRYEAFHDVEYTRDAIEAAVYQSS
                     RYITDRFLPDKAIDLVDEAGARAKLRHARPAEARVTAGGERAAVAVGTADGPGKHWRQ
                     EAGGWEGAGRREAGAPSSAGSRISVGKPEIDEIVAKWTGIPLSSVTLDEGARLMRMEA
                     ALHERVVSQDRAVSALARAIRRSRAGLKNPNRPVGSFVFLGPTGVGKTELARALAAYL
                     FGSDAALVRFDMSEYMEKHAVAKLIGSPPGYVGHDEGGQLTERVKRHPYSVVLLDEIE
                     KAHPDLFNILLQVLEDGHLTDGLGTRVSFKHAVIVMTSNIGARLIQKRASM"
BASE COUNT          200 a          536 c          510 g          258 t
ORIGIN      
        1 catggaggcc cgtttctgga tgagccgggc cccgatgttg gaggtcatga cgatgacggc
       61 gtgcttgaag ctcacgcgcg tgcccagccc gtcggtcagg tggccgtcct cgagaacctg
      121 gagcaggata ttgaacaggt ccgggtgggc tttctcaatc tcgtcgagca gtacgacgga
      181 gtacgggtgc cgcttgaccc gctcggtaag ctgtcctccc tcgtcgtggc cgacgtagcc
      241 gggcggcgac ccgatgagct tggccacggc gtgcttctcc atgtattccg acatgtcgaa
      301 gcgcaccagc gcggcatcgc tgccgaacaa gtacgccgcc agcgcgcgcg cgagctcggt
      361 cttgccgacg ccggtcgggc cgaggaagac gaagctgccg accggccggt tcgggttctt
      421 caagccggct cgcgaccggc ggatggcccg tgccagggcc gaaacggcgc gatcctggct
      481 gacgacgcgc tcgtgcagcg cggcttccat ccgcatcaac cgcgcgccct cgtccagcgt
      541 caccgacgac agggggatgc ccgtccactt cgcgacgatc tcgtcgatct ccggcttccc
      601 gacggagatc cggctgccgg ccgacgaggg ggccccggcc tcgcgcctgc cggcgccttc
      661 ccagcccccg gcctcctgcc gccagtgctt ccccggcccg tccgcggtgc ccacggcgac
      721 cgccgcccgt tccccgcccg ccgtcacgcg cgcctccgcc ggcctggcgt gacggagctt
      781 ggcccgggcg cccgcctcgt cgacgagatc gatcgctttg tccggcagga accggtccgt
      841 gatgtaccgg ctcgattggt acacggcggc ctcgatggcg tcccgcgtgt actcgacgtc
      901 gtgaaacgcc tcgtagcggt ctttgatgcc cagcaggatg tcgatcgtgt gcgcctcgtc
      961 gggcgcctcc accttgatgg cctggaaccg gcgctcgagc gagcggtccc gctcgatgta
     1021 cttccggtac tccgccggcg tggtcgagcc gatgcagcgg atctcgccgc gcgacagggc
     1081 gggcttcagg atgttggccg catcgagcga cccttcggcg gagccggcgc cgaccagggt
     1141 gtggagttcg tcgatgaaca cgatgacgtt cgtgtgctcg gcgagctcct tcatgatcgc
     1201 cttcaggcgc tcctcgaact gaccgcggta cttcgtgccg gcgacgacga gcgagatgtc
     1261 gagggccagg atgcgcttgt ccgccaggtc gcgcggcacc tgccccgcca cgatccgctg
     1321 ggcgagcccc tccacgatgg cggtcttgcc gacgcccggc tcgccgatga gcaccgggtt
     1381 gttcttggtg cggcggctga gcacctgaat cacgcggcgg acctcctcgt cgcggccgat
     1441 gacgggatcc agcttcccgg cctccgccag ggccgtgaag tccttggcgt acttctccag
     1501 ggcg
//
LOCUS       DAOSIF010000654         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_73282_length_1503_cov_3.897790, whole genome shotgun sequence.
ACCESSION   DAOSIF010000654 DAOSIF010000000
VERSION     DAOSIF010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73282_length_1503_cov_3.897790"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PLT35_14815"
     CDS             complement(<1..1261)
                     /locus_tag="PLT35_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30546.1"
                     /translation="MRLRLLKPATMVLLLAAGAAATHASTARFWRVSTRADLLKGEAT
                     GLSIDYDGRLVLGPASRTLLEPAAPFIWCLAPAPGGALYAGGGNDGLVWRVDRDGSSR
                     VVFDAAELEVHALAASPDGSLFVGTSPDGRVYRIGADGAATPFFDPDDTYIWALALDP
                     LGRLYVATGSKGAIYRVGRDGRSEMVFRAQAANVTSLAVDAAGRVFAGTESPGRIVRI
                     SADGRAFVLLDSPYRELRGLRVDTEGTLYAVAVDGKPETREAAQAASPPEPPRALPAA
                     AVSTEITAVTVVDASAPASGAPPAHAAAGLSPAAKGAVYRIDASGASEIVWSFPDDQP
                     FDLVLEDGGSLLVATGGAGRIYRLAGDPWKAMLVTRVDSRQATSLLRLEAATYVAASN
                     PARIVRIERASAAEGSYVSEAKDAGMAA"
     gene            complement(1291..>1503)
                     /locus_tag="PLT35_14820"
     CDS             complement(1291..>1503)
                     /locus_tag="PLT35_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30547.1"
                     /translation="AGRDAGAIVNGEILPSLPGSVLAVMQSDRGADVASALQTSVLGT
                     WEISTDEAVRGIRTITIPLAPPVPVP"
BASE COUNT          233 a          521 c          556 g          193 t
ORIGIN      
        1 aggccgccat acccgcgtcc ttggcctccg agacgtaaga gccttctgca gccgaggcgc
       61 gctcgatcct cacgatgcga gccgggttgg aggccgcgac ataggtcgcg gcctccagcc
      121 gaaggagcga cgtcgcctgc cgcgaatcca cgcgcgtgac gagcatcgct ttccacgggt
      181 caccggccaa tcgatagatc ctgcctgcgc cgcccgtggc gacgagaagg ctgcccccgt
      241 cttcgaggac gagatcgaac ggctgatcgt cggggaacga ccagacgatc tcgctggcgc
      301 ccgacgcatc gatgcggtag acggcccctt tggcggcggg ggagagaccg gcggctgcgt
      361 gggcgggcgg ggcgcctgag gcgggcgcgg acgcgtcgac aacagtgacg gcggtgatct
      421 cggtggaaac ggccgcggcc ggaagcgccc gaggaggctc gggcggcgac gccgcttggg
      481 ccgcttcacg ggtctcgggc ttgccgtcga ccgcgacggc gtaaagcgtt ccctcggtgt
      541 ccacccgcag tccgcgcagc tcgcggtacg gcgaatcgag aagcacgaac gccctgccgt
      601 cggccgaaat ccggacgatg cgccccggcg actcggtccc ggcgaagacc cggcccgcgg
      661 cgtcgacggc cagcgaggtc acgttggccg cctgcgcgcg gaagaccatt tcgctgcgcc
      721 cgtcgcgccc gacccggtag atcgcgccct tgctgccggt ggcgacatac aggcgtccca
      781 gcgggtcaag tgccagagcc caaatgtacg tgtcgtccgg atcgaagaag ggcgtggccg
      841 cgccgtccgc gccgatccgg tagactctgc cgtccggcga cgtcccgaca aacagcgagc
      901 cgtccggcga ggcggccagc gcatgcacct ccagttcggc cgcgtcgaac acgacgcggc
      961 tggacccgtc gcgatcgacc cgccagacga ggccgtcgtt gccgccgccg gcgtagagcg
     1021 cgccgcctgg ggccggcgcc aggcaccaga tgaacggggc ggccggctcg aggagcgtcc
     1081 gcgaggcggg gcccagcacg agcctgccgt cgtagtcgat tgacagcccg gttgcctcgc
     1141 ccttgagcag gtcggcccgc gtggacacgc gccagaaccg cgcggtcgac gcgtgggtcg
     1201 ctgcggcgcc ggcagcgagg agcagcacca tggtggccgg tttgagcagg cggagtctca
     1261 tgcgacagtg gcctcgcgcg accatcgtgg tcacggaacg ggtacgggag gggcgagcgg
     1321 aatggtgatc gtgcggatcc cgcggacagc ctcgtcggtc gagatctccc acgtgccgag
     1381 caccgacgtc tgaagagccg aggcgacgtc ggcgccgcgg tcgctctgca tgacggccag
     1441 cacggacccc ggcagcgagg ggaggatttc tccgttgaca atggcgccgg cgtcccggcc
     1501 cgc
//
LOCUS       DAOSIF010000655         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_73284_length_1503_cov_3.702348, whole genome shotgun sequence.
ACCESSION   DAOSIF010000655 DAOSIF010000000
VERSION     DAOSIF010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73284_length_1503_cov_3.702348"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLT35_14825"
     CDS             <1..552
                     /locus_tag="PLT35_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG30548.1"
                     /translation="HGDHYGGVPFFLMDAHYASGRTRPLVVAGPAGVEGAVARLAEAL
                     FPGVGMLPFRFPLTYLEWGERQEVPAGPAVVTPYEVRHSPALACFGLRVGMGGRVVAF
                     SGDTEWTDALAPLSGDADLFVCECFGLDAAPPHHLDFRTLQRHRHALTCRRLVLTHMG
                     EDMLERAPTLGVETARDGLCLTL"
     gene            complement(676..>1503)
                     /locus_tag="PLT35_14830"
     CDS             complement(676..>1503)
                     /locus_tag="PLT35_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG30549.1"
                     /translation="ELMDYLEVVGRGQPITAPPLPLVAVPTTAGTGSEVTRNAVVTVP
                     GRRVKASLRSPLMLPRAAVVDPRLTYGLPPALTASTGLDALTQLIEPFVSLRANPVAD
                     ALCREGLARASRSLCRACEDGRDAAARTDMSLASLLGGMALASAGLGAVHGLAAPIGG
                     MFRAPHGAVCAALLPHVMAANLRALGEGRGRPDALGRMAEIGRLVTRSPEATAEDAVA
                     WIRGTVARLEIPGLSRYGMAADALPEVAAQGLQASSMKGNPVALSAAELVGILEAAL"
BASE COUNT          188 a          567 c          546 g          202 t
ORIGIN      
        1 cacggagacc actacggcgg cgtgcctttc ttcctgatgg acgcccacta cgccagcggg
       61 cgcacgcgcc cgctcgtcgt cgccggaccg gccggcgtcg agggcgccgt ggcccggctg
      121 gcggaggccc tgttcccggg cgtcggcatg ctgccgttca ggttccccct gacctacctg
      181 gaatggggcg aacggcagga ggttccggcg ggcccggcgg tggtcacgcc gtacgaggtc
      241 cgtcactcgc ccgcgctcgc ctgcttcggc ctgcgcgtgg gcatgggggg acgcgtcgtc
      301 gcgttctcgg gcgacaccga atggaccgac gccctggcgc cgctctcggg cgatgccgac
      361 ctcttcgtct gcgagtgctt cggactcgat gccgcgccgc cgcaccatct ggatttccgg
      421 acgctccagc ggcaccgcca cgcactgacc tgccgccggc tcgtcctcac gcacatgggc
      481 gaagacatgc tcgaacgcgc gcccacgctc ggcgtcgaga ccgcccgcga cggcctctgc
      541 ctcacgctgt gagggggccg ggactcgggg ctcggggact gaggactcgg gattcggggc
      601 tcgggcctcg acagcgagac acggcgctcc attccgggtt ctgaacaccg ggttctgtac
      661 cgcacctccc gccgattaga gagcggcctc cagaatcccg acgagttcgg ctgccgacaa
      721 cgcgaccggg ttgcccttca tgctgctcgc ctgcagtccc tgcgcggcca cctccggcag
      781 cgcgtccgcg gccattccgt agcgggacag ccccgggatc tcgagccgcg ccaccgtccc
      841 gcgaatccag gccaccgcgt cttccgcggt cgcctcgggg gatcgcgtca cgaggcgccc
      901 gatctcggcc atgcggccga gcgcgtccgg ccggcctcgg ccttcgccga gggcgcggag
      961 gttggcggcc atgacgtgcg gcagcagcgc cgcgcacacg gcgccgtgcg gcgcgcggaa
     1021 catgccgccg atgggggccg ccagcccgtg caccgccccg agcccggcgc tggccagcgc
     1081 catcccgccg agcaggctgg cgagcgacat gtcggttcgc gcggcggcgt cgcggccgtc
     1141 ctcgcaggcg cggcagagcg aacgcgacgc gcgcgccagg ccttcgcggc acagcgcgtc
     1201 ggcgaccggg ttggcgcgca gggacacgaa cggctcgatg agctgcgtca gcgcgtccag
     1261 tcccgtcgag gccgtcagcg ccgggggcag gccgtaggtc aggcgcggat cgaccaccgc
     1321 ggcgcggggc agcatgagcg ggctgcgcag gctggccttc acgcggcggc ccggaaccgt
     1381 cacgacggcg ttccgcgtga cctccgagcc ggttcccgcc gttgtgggca cggccacgag
     1441 cggaaggggc ggcgccgtga tcggctggcc gcgcccgacc acctcgaggt aatccatcag
     1501 ctc
//
LOCUS       DAOSIF010000656         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1708
            NODE_73373_length_1502_cov_3.115411, whole genome shotgun sequence.
ACCESSION   DAOSIF010000656 DAOSIF010000000
VERSION     DAOSIF010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863237
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:13:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 6 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 5 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73373_length_1502_cov_3.115411"
                     /isolate="AMR_MDS_1708"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLT35_14835"
     CDS             complement(<1..798)
                     /locus_tag="PLT35_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG30550.1"
                     /translation="MVNVLGPHDEFLRILERDLDAEIHVRGNEITLSGTPTGVAQASD
                     VMTELVTIVRTGQGLTTDAVERLIAMASEHDHPAEVLTQNILSTRGRTIRPKTLNQKR
                     YVDAIDAHTIVFGIGPAGTGKTYLAMAKAVQALQSRQVNRIIMTRPAIEAGERLGFLP
                     GTLSDKIDPYLRPLYDALHDMVDPDSVPKLLAANTIEVAPLAYMRGRTLNDAFIILDE
                     AQNTSMEQMKMFLTRLGFNSRIVVTGDVTQVDLPNGMKSGLREVQGIL"
     gene            complement(916..>1502)
                     /locus_tag="PLT35_14840"
     CDS             complement(916..>1502)
                     /locus_tag="PLT35_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG30551.1"
                     /translation="RVLLYGIVGALVLRGIFTLRRFELDWYYNGGAGHLAPQWRESFL
                     APLGGTSFAGDAMARYHELLFDPDPAVHGPAGVAWTTWEAATSYLHFRPDAVEEFADP
                     AFALAFARIENHFFTHGGWLDEGQLIAEASKLADIPGVIVQGAYDLCCPPVTAWDLHR
                     AWPQARFELVHAGHASFEPAITDALVRATDAFAA"
BASE COUNT          215 a          516 c          528 g          243 t
ORIGIN      
        1 gaggatgccc tgcacctccc gcaggccgga cttcatgccg ttcggcaggt cgacctgggt
       61 gacgtcaccc gtgacgacga tgcggctgtt gaaccccagc cgcgtcagga acatcttcat
      121 ctgctccatc gacgtgttct gcgcctcgtc gaggatgatg aacgcgtcgt tcagcgtccg
      181 cccgcgcatg taggcgagcg gcgcgacctc gatcgtgttc gcggccagca gcttcgggac
      241 gctgtcgggg tcgaccatgt cgtgcaaggc gtcgtagagc ggccgcaggt acgggtcgat
      301 cttgtccgac agcgtgcccg gcaggaaccc cagccgctcg cccgcctcga tcgccgggcg
      361 cgtcatgatg atccggttca cctgccgcga ctgcaacgcc tggacggcct tcgccatcgc
      421 gaggtacgtc ttgcccgtcc cggccggccc gatgccgaac acgatggtgt gggcgtcgat
      481 ggcgtccacg taccgcttct ggttgagggt cttcgggcgg atggtcctcc cgcgcgtcga
      541 caggatgttc tgcgtcagca cctcggccgg gtgatcatgc tcggacgcca tcgcgatcag
      601 ccgctcgacg gcgtccgtcg tgaggccctg ccctgtgcgg acgatcgtca ccagttccgt
      661 catcacgtcg gacgcctgcg cgacgcccgt cggcgtcccc gacagggtga tctcgttgcc
      721 acggacgtgg atctcggcgt ccagatcgcg ctcaaggatg cgaaggaact cgtcgtgcgg
      781 gcccagcacg ttgaccatgc cgatgttcgc gcctaccgcg atgcgcctcg tcgcgaccgc
      841 ttcgcctgcc gtattcgtca gtgggaaacc ccttgtctgt ggacgcctcg cggcgtcggc
      901 tccgtcggcg gcagtctacg cggcgaacgc gtccgtggcc cggacgagcg cgtccgtgat
      961 cgcgggctcg aaagacgcgt gcccggcgtg caccagttcg aaccgggcct gcggccaggc
     1021 gcgatgcagg tcccaggcgg tgaccggcgg gcagcacagg tcgtaggcgc cctggacgat
     1081 cacgccgggg atgtcggcga gcttggacgc ctccgcgatc agctggccct cgtccagcca
     1141 gccgccgtgg gtgaagaagt ggttctcgat ccgcgcgaag gccagcgcga aggccgggtc
     1201 ggcgaactcc tcgactgcgt ccgggcggaa gtgcaggtag gaggtcgcgg cctcccaggt
     1261 ggtccaggcg accccggccg ggccgtgcac ggccgggtcg ggatcgaaca gcagctcgtg
     1321 gtagcgggcc atggcgtcgc cggcgaacga cgtgcccccc agcggggcca ggaagctctc
     1381 gcgccactgc ggcgccaggt gcccggcgcc gccgttgtag taccagtcga gctcgaagcg
     1441 gcgcagggtg aagatgccgc gcaggaccag ggcgccgacg atcccgtaga gcagcacccg
     1501 ct
//
